Microsphere resin chromatography combined with microbial biotransformation for the separation and purification of salvianolic acid B in aqueous extract of roots of Salvia multiorrihza Bunge.
Salvianolic acid B was separated and purified from Salvia miltiorrhiza Bunge (danshen) by microbial transformation together with chromatography of microsphere resin. The aqueous extract of danshen was transformed by Fusarium graminearum in a bioreactor containing phosphate buffer (PBS), in which rosmarinic acid was transformed into danshensu and caffeic acid and the yield of salvianolic acid B was higher than 85%. After biotransformation, salvianolic acid B was purified by microsphere resin. A parallel test for making a comparison of microsphere resin chromatography between elution by methanol water solution and water was done. The purity of salvianolic acid B was up to 95% at the yield of 62% when impurities and salvianolic acid B were eluted by 45% and 55% methanol solution respectively. The purity of salvianolic acid B was up to 99% at the yield of 90% when distilled water was used to elute the impurities and salvianolic acid B. The total yield of salvianolic acid B was up to 75% at the purity over 99% while biotransformation combined with microsphere resin chromatography by water elution. Microbial biotransformation together with water elution of microsphere resin supplied an efficient method to eliminate the micromolecular impurities and a possible method to purify water-soluble compounds in traditional Chinese medicine.
Kan
Shidong
S
Department of Microbiology, Life Science College, Nanjing Agricultural University, Nanjing 210095, China.
Li
Ji'an
J
Huang
Weiyi
W
Shao
Lei
L
Chen
Daijie
D
D000327:Adsorption; D001572:Benzofurans / Q000032:analysis / Q000302:isolation & purification* / Q000378:metabolism; D001711:Biotransformation; D002853:Chromatography, Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000032:analysis / Q000302:isolation & purification* / Q000378:metabolism; D005670:Fusarium / Q000378:metabolism*; D010618:Phenanthrolines / Q000032:analysis / Q000302:isolation & purification* / Q000378:metabolism; D018517:Plant Roots / Q000737:chemistry / Q000378:metabolism; D012117:Resins, Synthetic / Q000737:chemistry; D027561:Salvia miltiorrhiza / Q000737:chemistry* / Q000378:metabolism; D053630:Depsides
D001572:Benzofurans; D004365:Drugs, Chinese Herbal; D010618:Phenanthrolines; D012117:Resins, Synthetic; C028972:dan-shen root extract; C076944:salvianolic acid B; D053630:Depsides