Fed-batch fermentation of Tuber melanosporum for the hyperproduction of mycelia and bioactive Tuber polysaccharides.
For the first time, a fed-batch fermentation process of Tuber melanosporum was developed for the efficient production of bioactive mycelia and Tuber polysaccharides. Each 1.67 g/L of peptone and 8.33 g/L of yeast extract were added on day 3, 6, and 9, respectively, and sucrose was fed to maintain its concentration around 35-5 g/L when its residual level decreased to 10-5 g/L. Then, the maximal biomass, the production of extracellular polysaccharides (EPS) and intracellular polysaccharides (IPS) reached 53.72+/-2.57 g DW/L, 7.09+/-0.62 and 4.43+/-0.21 g/L, respectively. Compared with the batch culture conducted in the enriched medium, the biomass, the production of EPS and IPS were enhanced by 55.8%, 222.3% and 103.2%, respectively. Not only the cell density but also the production of EPS and IPS were the highest ever reported in truffle fermentation, and the biomass was also the highest as ever reported in mushroom fermentation.
Liu
Qiao-Ning
QN
Key Laboratory of Fermentation Engineering (Minister of Education), Hubei Provincial Key Laboratory of Industrial Microbiology, College of Bioengineering, Hubei University of Technology, Wuhan 430068, China.
Liu
Rui-Sang
RS
Wang
Yi-Hua
YH
Mi
Zhi-Yuan
ZY
Li
Dong-Sheng
DS
Zhong
Jian-Jiang
JJ
Tang
Ya-Jie
YJ
D000363:Agaricales / Q000378:metabolism*; D018533:Biomass; D019149:Bioreactors / Q000382:microbiology*; D001709:Biotechnology / Q000379:methods*; D002241:Carbohydrates / Q000737:chemistry; D002244:Carbon / Q000737:chemistry; D018929:Cell Culture Techniques / Q000379:methods*; D003470:Culture Media / Q000378:metabolism; D005285:Fermentation; D007700:Kinetics; D009584:Nitrogen / Q000737:chemistry; D010461:Peptones / Q000737:chemistry; D011134:Polysaccharides / Q000737:chemistry* / Q000378:metabolism*; D013395:Sucrose / Q000737:chemistry
D002241:Carbohydrates; D003470:Culture Media; D010461:Peptones; D011134:Polysaccharides; D013395:Sucrose; D002244:Carbon; D009584:Nitrogen