Incorporation of an inducible nucleotide analog into DNA by DNA polymerases.
Non-natural nucleotides with diverse functionalities are highly useful in many areas of basic research and practical applications. We have previously developed an efficient method for post-synthetic modifications of 2-amino-6-vinylpurine (AVP)-containing oligonucleotides, which permits conjugations of a variety of useful functional appendages to the AVP moiety in DNA. Here we report an investigation on the ability of various DNA polymerases to use 5'-triphosphate of 2'-deoxyribosyl-2-amino-6-(2-methylthioethyl)purine (a stable precursor of AVP) as the substrate for templated DNA synthesis.
Ali
Md Monsur
MM
Department of Biochemistry and Biomedical Sciences, McMaster University, 1200 Main Street West, Hamilton, Canada ON L8N 3Z5.
Imoto
Shuhei
S
Li
Yingfu
Y
Sasaki
Shigeki
S
Nagatsugi
Fumi
F
D001483:Base Sequence; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism*; D009838:Oligodeoxyribonucleotides / Q000096:biosynthesis* / Q000737:chemistry; D011685:Purine Nucleotides / Q000138:chemical synthesis* / Q000737:chemistry; D013698:Templates, Genetic
C528377:2'-deoxyribosyl-2-amino-6-(2-methylthioethyl)purine nucleoside triphosphate; D009838:Oligodeoxyribonucleotides; D011685:Purine Nucleotides; D004259:DNA-Directed DNA Polymerase