Characterization of function and regulation of miR-24-1 and miR-31.
To date, numerous microRNAs (miRNAs) have been discovered. However, the function of these miRNAs is largely unknown. While our knowledge of miRNA post-transcriptional processing has greatly expanded in recent years, we have a limited understanding of the regulation and transcription of miRNA genes. In this study, we characterized two BMP-2 upregulated miRNAs, miR-24-1 and miR-31, in mesenchymal stem cells and showed their opposing function in controlling cellular proliferation, and adipogenesis. Furthermore, we are the first to identify and characterize mouse intronic miR-23b~27b~24-1 and intergenic miR-31 genes. Moreover, we found that pri-miR-23b, pri-miR-27b, and pri-miR-24-1 are transcribed independently and their expression profiles are unique when cells are treated with BMP-2, even though they are located closely together.
Sun
Fenyong
F
Institute of Bioengineering, College of Life Science and Technology, Jinan University, Guangzhou City, 510632 Guangdong Province, PR China.
Wang
Jiayi
J
Pan
Qiuhui
Q
Yu
Yongchun
Y
Zhang
Yue
Y
Wan
Yang
Y
Wang
Ju
J
Li
Xiaoyan
X
Hong
An
A
D050156:Adipogenesis / Q000235:genetics; D000818:Animals; D055396:Bone Morphogenetic Protein 2 / Q000378:metabolism* / Q000494:pharmacology; D002460:Cell Line; D049109:Cell Proliferation; D005786:Gene Expression Regulation / Q000187:drug effects; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D051379:Mice; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D011401:Promoter Regions, Genetic / Q000187:drug effects; D014158:Transcription, Genetic
C525719:Bmp2 protein, mouse; D055396:Bone Morphogenetic Protein 2; D035683:MicroRNAs; C529080:Mirn24 microRNA, mouse; C539917:Mirn31 microRNA, mouse