title: Rapid screening for anthocyanins and anthocyanin dimers in crude grape extracts by high performance liquid chromatography coupled with diode array detection and tandem mass spectrometry.
abstract: A rapid and efficient method using high performance liquid chromatography coupled with diode array detection and tandem mass spectrometry (HPLC-DAD-MS/MS) for fast screening large numbers of anthocyanins and anthocyanin dimers in different grape skin extracts, without further sample clean-up procedures, was developed. A good separation of most detected anthocyanins was achieved in a run time of 15 min. Identification of anthocyanin pigments required a combination of several information: UV-vis spectra, MS and MS/MS spectra, and elution pattern. Many compounds have been here detected for the first time and their structures tentatively elucidated.
authors.affiliation: Department of Food Science, University of Foggia-Via Napoli 25, Foggia, Italy. s.pati@unifg.it
authors.forename: Sandra
authors.initials: S
authors.lastname: Pati
authors.forename: Maria Teresa
authors.initials: MT
authors.lastname: Liberatore
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Gambacorta
authors.forename: Donato
authors.initials: D
authors.lastname: Antonacci
authors.forename: Ennio
authors.initials: E
authors.lastname: La Notte
mesh_terms: D000872:Anthocyanins / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D019281:Dimerization; D010936:Plant Extracts / Q000737:chemistry; D053719:Tandem Mass Spectrometry / Q000295:instrumentation / Q000379:methods*; D027843:Vitis / Q000737:chemistry*
chemical_list: D000872:Anthocyanins; D010936:Plant Extracts