[Construction and expression of spap/A eukaryotic expression plasmid of Streptococcus mutans in mammalian cells].
OBJECTIVE
The study is aimed to evaluate the expression of recombinant plasmid pVAX1-spap/A of surface protein antigen A of Streptococcus mutans in mammalian cells COS-7.


METHODS
The eukaryotic plasmid carrying encoding gene of spap/A of Streptococcus mutans was constructed and the plasmid introduced into COS-7 cells by lipofectamine reagent. The transient expressed protein was detected by immunochemistry technique in COS-7 cells.


RESULTS
Positive expression was detected in plasma of the cells which were transfected with recombinant plasmid pVAX1-spap/A. The cells which were transfected with pVAX1 were negative.


CONCLUSIONS
Spap/A can translate and express in COS-7 cells after transfected with recombinant plasmid pVAX1-spap/A. The expressed protein locates in the plasma and the protein is able to combine with anti-spap/A antibody. The expressed protein has the antigenicity and recombinant plasmid pVAX1-spap/A is a candidate vaccine.
Qu
Yun-peng
YP
The Affiliated Stomatological Hospital, Wenzhou Medical College, Wenzhou 325027, China. quyunpeng618@163.com
Liu
Jian-guo
JG
Yang
Ping
P
Yang
De-qin
DQ
D000818:Animals; D019556:COS Cells; D002522:Chlorocebus aethiops; D010957:Plasmids; D013295:Streptococcus mutans; D014162:Transfection