title: Differential participation of angiotensin II type 1 and 2 receptors in the regulation of cardiac cell death triggered by angiotensin II.
abstract: BACKGROUND
  The Angiotensin II (Ang II) type 1 (AT(1)R) and type 2 (AT(2)R) receptors are increased in the heart following myocardial infarction and dilated cardiomyopathy, yet their contribution at a cellular level to compensation and/or failure remains controversial.
  
  
  METHODS
  We ectopically expressed AT(1)R and AT(2)R in cultured adult rat cardiomyocytes and cardiac fibroblasts to investigate Ang II-mediated cardiomyocyte hypertrophy and cardiac cell viability.
  
  
  RESULTS
  In adult rat cardiomyocytes, Ang II did not induce hypertrophy via the AT(1)R, and no effect of Ang II on cell viability was observed following AT(1)R or AT(2)R expression. In adult rat cardiac fibroblasts, Ang II stimulated cell death by apoptosis via the AT(1)R (but not the AT(2)R), which required the presence of extracellular calcium, and induced a rapid dissipation of mitochondrial membrane potential, which was significant from 8 h.
  
  
  CONCLUSIONS
  We conclude that Ang II/AT(1)R triggers apoptosis in adult rat cardiac fibroblasts, which is dependent on Ca2+ influx.
authors.affiliation: Centro FONDAP Estudios Moleculares de la Célula, Facultad Ciencias Químicas y Farmacéuticas, Universidad de Chile, Santiago, Chile.
authors.forename: Pablo
authors.initials: P
authors.lastname: Aránguiz-Urroz
authors.forename: Dagoberto
authors.initials: D
authors.lastname: Soto
authors.forename: Ariel
authors.initials: A
authors.lastname: Contreras
authors.forename: Rodrigo
authors.initials: R
authors.lastname: Troncoso
authors.forename: Mario
authors.initials: M
authors.lastname: Chiong
authors.forename: José
authors.initials: J
authors.lastname: Montenegro
authors.forename: Daniel
authors.initials: D
authors.lastname: Venegas
authors.forename: Christian
authors.initials: C
authors.lastname: Smolic
authors.forename: Pedro
authors.initials: P
authors.lastname: Ayala
authors.forename: Walter G
authors.initials: WG
authors.lastname: Thomas
authors.forename: Sergio
authors.initials: S
authors.lastname: Lavandero
authors.forename: Guillermo
authors.initials: G
authors.lastname: Díaz-Araya
mesh_terms: D015059:1-Sarcosine-8-Isoleucine Angiotensin II / Q000378:metabolism; D000256:Adenoviridae / Q000235:genetics; D000804:Angiotensin II / Q000494:pharmacology*; D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D002118:Calcium / Q000502:physiology; D016923:Cell Death / Q000187:drug effects; D002478:Cells, Cultured; D008297:Male; D032383:Myocytes, Cardiac / Q000187:drug effects* / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D044140:Receptor, Angiotensin, Type 1 / Q000502:physiology*; D044139:Receptor, Angiotensin, Type 2 / Q000502:physiology*; D014161:Transduction, Genetic
chemical_list: D044140:Receptor, Angiotensin, Type 1; D044139:Receptor, Angiotensin, Type 2; D000804:Angiotensin II; D015059:1-Sarcosine-8-Isoleucine Angiotensin II; D002118:Calcium