[Synthesis of N-acyl-homoserine lactones, phenazines, some enzymatic activities and antifungal activity of Pseudomonas chlororaphis 449 cells carrying an inactivated rpoS gene].
Inactivation of the rpoS gene encoding for sigma S subunit of RNA polymerase of the rhizospheric strain Pseudomonas chlororaphis 449 results in a sharp decrease (5-8 fold) of phenazine antibiotics synthesis, decline of acid and alkaline phosphatases (pH 2.5 and 8.8, respectively) activities and antagonistic activity of this strain against phytopathogenic fungus Rhizoctonia solani. A mutation in the rpoS gene causes a small decrease of lipase and proteolytic activities in supernatants of Pseudomonas chlororaphis 449 cultures, as well as does not substantially affect the synthesis of three types of N-acyl-homoserinelactones that are signal molecules of Quorum Sensing regulation, and the capacity of bacteria to motility on the surface of the medium (swarming).
Lipasova
V A
VA
Atamova
E E
EE
Khmel'
I A
IA
D054742:Acyl-Butyrolactones / Q000378:metabolism*; D000935:Antifungal Agents / Q000378:metabolism*; D001426:Bacterial Proteins / Q000378:metabolism*; D010619:Phenazines / Q000378:metabolism*; D011549:Pseudomonas / Q000378:metabolism*; D053038:Quorum Sensing / Q000502:physiology*; D012232:Rhizoctonia / Q000254:growth & development; D012808:Sigma Factor / Q000378:metabolism*
D054742:Acyl-Butyrolactones; D000935:Antifungal Agents; D001426:Bacterial Proteins; D010619:Phenazines; D012808:Sigma Factor; C069567:sigma factor KatF protein, Bacteria