Association of TRB3 gene Q84R polymorphism with type 2 diabetes mellitus in Chinese population.
BACKGROUND
TRB3, a human homolog of Drosophila Tribbles, has been shown as a critical negative regulator of Akt (also known as protein kinase B), which is a key component in insulin signaling. In addition, TRB3 is another PPAR-target gene and functions as an important link between glucose and lipid metabolism. The Q84R polymorphic variant of TRB3 has been linked to insulin resistance and related clinical outcomes. However, it is unclear whether this polymorphism is associated with type 2 diabetes mellitus (T2DM) in the Chinese population.


METHODS
In this study, we genotyped Q84R polymorphism in 177 patients with T2DM and 245 control subjects in Chinese population by using the polymerase chain reaction/ligase detection reaction (PCR/LDR) assay.


RESULTS
No significant difference in the Q84R genotype frequency was observed between T2DM patients and controls (P = 0.642). In T2DM group, the Q84R variant in cases was associated with higher FINS, higher HOMA-IR, and lower LnISI (P = 0.003, 0.001, and 0.001, respectively). However, the changes in HOMA-IR and LnISI were not significant in controls (the P value is 0.098 and 0.203, respectively). In addition, FINS levels were also significantly increased from Q84Q to R84 in controls (P = 0.036).


CONCLUSIONS
Our data indicate that the TRB3 Q84R polymorphism is not associated with T2DM in Chinese population. However, the Q84R variant is associated with insulin resistance among T2DM patients in Chinese population.
Shi
Zhiyong
Z
Lanzhou University, Lanzhou City 730000, Gansu Province, People's Republic of China.
Liu
Jing
J
Guo
Qian
Q
Ma
Xiaoqin
X
Shen
Linna
L
Xu
Sanni
S
Gao
Hongxia
H
Yuan
Xinjian
X
Zhang
Junling
J
D000328:Adult; D000368:Aged; D019943:Amino Acid Substitution / Q000235:genetics; D001120:Arginine / Q000235:genetics; D016022:Case-Control Studies; D018797:Cell Cycle Proteins / Q000235:genetics*; D002681:China; D003924:Diabetes Mellitus, Type 2 / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D008040:Genetic Linkage; D005828:Genetics, Population; D018698:Glutamic Acid / Q000235:genetics; D006801:Humans; D007333:Insulin Resistance / Q000235:genetics; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D017346:Protein Serine-Threonine Kinases / Q000235:genetics* / Q000037:antagonists & inhibitors; D012097:Repressor Proteins / Q000235:genetics*
D018797:Cell Cycle Proteins; D012097:Repressor Proteins; C474831:TRIB3 protein, human; D018698:Glutamic Acid; D001120:Arginine; D017346:Protein Serine-Threonine Kinases