title: Immunophenotyping and gene rearrangement analysis in dogs with lymphoproliferative disorders characterized by small-cell lymphocytosis.
abstract: Lymphocytosis caused by neoplastic proliferation of small lymphocytes is occasionally difficult to distinguish by morphological examination from nonneoplastic lymphocytosis. To examine the clinical utility of gene rearrangement analysis for demonstrating neoplastic proliferation of small lymphocytes, gene rearrangement analysis was performed in comparison with immunophenotyping using peripheral lymphocytes in dogs with small lymphocytosis. Thirty-one dogs with small-cell lymphocytosis (8,100-884,300/microl) were enrolled. By immunophenotyping, lymphocytosis of all dogs was suggested to be neoplastic in nature based on the detection of marked expansion of phenotypically homogeneous lymphocytes or the presence of an aberrant antigen-expressing population of lymphocytes. In contrast, gene rearrangement analysis represented clonality in 27 dogs (detection rate of 87%). From the present study, gene rearrangement analysis was considered to be worthwhile to strengthen the evidence of neoplastic proliferation of small lymphocytes when coupled with immunophenotyping and to be a suitable diagnostic substitute if immunophenotyping is not available in clinical practice.
authors.affiliation: Department of Veterinary Clinical Pathology, Nippon Veterinary and Life Science University, Tokyo, Japan.
authors.forename: Hiroko
authors.initials: H
authors.lastname: Yagihara
authors.forename: Yosuke
authors.initials: Y
authors.lastname: Uematsu
authors.forename: Ayumi
authors.initials: A
authors.lastname: Koike
authors.forename: Kyoichi
authors.initials: K
authors.lastname: Tamura
authors.forename: Mayu
authors.initials: M
authors.lastname: Isotani
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Yamaguchi
authors.forename: Kenichiro
authors.initials: K
authors.lastname: Ono
authors.forename: Tsukimi
authors.initials: T
authors.lastname: Washizu
authors.forename: Makoto
authors.initials: M
authors.lastname: Bonkobara
mesh_terms: D000818:Animals; D004247:DNA / Q000737:chemistry / Q000235:genetics; D004283:Dog Diseases / Q000097:blood* / Q000235:genetics / Q000276:immunology; D004285:Dogs; D005260:Female; D005434:Flow Cytometry / Q000662:veterinary; D015334:Gene Rearrangement, gamma-Chain T-Cell Antigen Receptor; D016130:Immunophenotyping / Q000662:veterinary; D008218:Lymphocytosis / Q000097:blood / Q000235:genetics / Q000276:immunology / Q000662:veterinary*; D008232:Lymphoproliferative Disorders / Q000097:blood / Q000235:genetics / Q000276:immunology / Q000662:veterinary*; D008297:Male; D016133:Polymerase Chain Reaction / Q000662:veterinary
chemical_list: D004247:DNA