title: [Expression of multiepitope antigenic gene of Toxoplasma gondii in transgenic tomatoes].
abstract: OBJECTIVE
  To obtain the transgenic tomato plant expressed multiepitope antigenic gene of Toxoplasma gondii (Tg-MAG).
  
  
  METHODS
  Tg-MAG under the control of E35S promoter, E35S-E81.1 chimeric promoter and E8 2.2 promoter in the plant expression vectors was transferred into the tomato cotyledons and hypocotyls via Agrobacterium-mediated T-DNA transformation. The candidate buds were obtained and elongated by kanamycin resistant screening. After further rooting selection, the selected tomato lines were trained and transplanted to soil and grown in a greenhouse till blossoming and bearing fruit. The transgenic tomato plants were identified using PCR, RT-PCR and Western blotting.
  
  
  RESULTS
  Tg-MAG was introduced into the transgenic plant genomic DNA and transcribed correctly at expected size 360 bp identified by PCR and RT-PCR. Western blotting results indicated that the recombinant Tg-MAG from 8 transgenic lines, expressed in tomato fruits at expected size 11900, 2 of them showed strong reaction band with HRP labeled McAb K7H3 against the major surface antigen 1( SAG1) of T. gondii tachyzoites.
  
  
  CONCLUSIONS
  Tg-MAG transgenic tomato lines were obtained. Tg-MAG recombinant protein with good immune activity was expressed successfully in transgenic tomato fruits.
authors.affiliation: Department of Parasitology, School of Public Health and Tropical Medicine, Southern Medical University, Guangzhou 510515, China.
authors.forename: Xiao-hong
authors.initials: XH
authors.lastname: Zhou
authors.forename: Xiao-qin
authors.initials: XQ
authors.lastname: Huang
authors.forename: Ren-jia
authors.initials: RJ
authors.lastname: Zhong
authors.forename: Qiao-zhen
authors.initials: QZ
authors.lastname: Zhu
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhao
authors.forename: Yu-qi
authors.initials: YQ
authors.lastname: Guo
authors.forename: Xiao-guang
authors.initials: XG
authors.lastname: Chen
mesh_terms: D000818:Animals; D000953:Antigens, Protozoan / Q000235:genetics*; D003001:Cloning, Molecular; D015870:Gene Expression; D005822:Genetic Vectors; D018551:Solanum lycopersicum / Q000235:genetics / Q000378:metabolism; D030821:Plants, Genetically Modified / Q000235:genetics / Q000378:metabolism*; D014122:Toxoplasma / Q000235:genetics* / Q000276:immunology
chemical_list: D000953:Antigens, Protozoan