title: Isolation and characterization of Rhodococcus sp. Y22 and its potential application to tobacco processing.
abstract: A novel nicotine-degrading bacterium, strain Y22, was isolated and identified as Rhodococcus sp. Y22 based on its 16S rDNA sequence and morphological and biochemical features. The isolate could utilize nicotine as the sole source of carbon and nitrogen. Nicotine (1.0g/L) was degraded by Rhodococcus sp. Y22 within 52h at 28 degrees C and pH 7.0. Preparation of resting cells from nicotine-induced cultures was found to rapidly and efficiently degrade nicotine from solutions as well as from tobacco leaves. Therefore, Rhodococcus sp. Y22 has the potential to degrade nicotine during tobacco leave processing.
authors.affiliation: Laboratory for Conservation and Utilization of Bio-resources, and Key Laboratory for Microbial Resources of the Ministry of Education, Yunnan University, Kunming, People's Republic of China.
authors.forename: Xiao-Wei
authors.initials: XW
authors.lastname: Gong
authors.forename: Jin-Kui
authors.initials: JK
authors.lastname: Yang
authors.forename: Yan-Qin
authors.initials: YQ
authors.lastname: Duan
authors.forename: Jin-Yan
authors.initials: JY
authors.lastname: Dong
authors.forename: Wei
authors.initials: W
authors.lastname: Zhe
authors.forename: Le
authors.initials: L
authors.lastname: Wang
authors.forename: Qing-Hua
authors.initials: QH
authors.lastname: Li
authors.forename: Ke-Qin
authors.initials: KQ
authors.lastname: Zhang
mesh_terms: D015373:Bacterial Typing Techniques; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D006863:Hydrogen-Ion Concentration; D007218:Industrial Microbiology; D008969:Molecular Sequence Data; D009538:Nicotine / Q000378:metabolism*; D010802:Phylogeny; D018515:Plant Leaves / Q000378:metabolism; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012240:Rhodococcus / Q000145:classification* / Q000166:cytology / Q000302:isolation & purification / Q000378:metabolism*; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D013696:Temperature; D013997:Time Factors; D014026:Nicotiana / Q000378:metabolism
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D009538:Nicotine