title: p110gamma and p110delta isoforms of phosphoinositide 3-kinase differentially regulate natural killer cell migration in health and disease.
abstract: The mechanisms that regulate NK cell trafficking are unclear. Phosphoinositide-3 kinases (PI3K) control cell motility and the p110gamma and p110delta isoforms are mostly expressed in leukocytes, where they transduce signals downstream of G protein coupled receptors (GPCR) or tyrosine kinase receptors, respectively. Here, we set out to determine the relative contribution of p110gamma and p110delta to NK cell migration in mice. Using a combination of single-cell imaging analysis of transgenic cells reporting on PI3K activity in real time and small molecule inhibitors of p110gamma and p110delta, we show here that the tyrosine-kinase coupled p110delta is linked to GPCR signaling and, depending on the GPCR, may even be preferentially activated over p110gamma. Using gene-targeted mice, we showed that both isoforms were essential for NK cell chemotaxis to CXCL12 and to CCL3 and, in vivo, for normal NK cell migration during pregnancy and to the inflamed peritoneum. By contrast, only p110delta was indispensable for chemotaxis to S1P and CXCL10 and for NK cell distribution throughout lymphoid and nonlymphoid tissues and for extravasation to tumors. These results implicate p110delta downstream of GPCRs in NK cells and highlight its nonredundant role as a key regulator of NK cell trafficking in health and disease.
authors.affiliation: Laboratory of Lymphocyte Signalling, The Babraham Institute, CB22 3AT Cambridge, United Kingdom.
authors.forename: Aurore
authors.initials: A
authors.lastname: Saudemont
authors.forename: Fabien
authors.initials: F
authors.lastname: Garçon
authors.forename: Hakim
authors.initials: H
authors.lastname: Yadi
authors.forename: Marta
authors.initials: M
authors.lastname: Roche-Molina
authors.forename: Nayoung
authors.initials: N
authors.lastname: Kim
authors.forename: Anne
authors.initials: A
authors.lastname: Segonds-Pichon
authors.forename: Alfonso
authors.initials: A
authors.lastname: Martín-Fontecha
authors.forename: Klaus
authors.initials: K
authors.lastname: Okkenhaug
authors.forename: Francesco
authors.initials: F
authors.lastname: Colucci
mesh_terms: D000818:Animals; D054405:Chemokine CCL3; D054357:Chemokine CXCL10; D054377:Chemokine CXCL12; D002634:Chemotaxis, Leukocyte / Q000276:immunology*; D058534:Class I Phosphatidylinositol 3-Kinases; D004195:Disease Models, Animal; D005260:Female; D007249:Inflammation; D007694:Killer Cells, Natural / Q000276:immunology*; D008113:Liver Neoplasms; D051379:Mice; D018345:Mice, Knockout; D010537:Peritoneum / Q000473:pathology; D019869:Phosphatidylinositol 3-Kinases / Q000276:immunology*; D011247:Pregnancy; D043562:Receptors, G-Protein-Coupled / Q000378:metabolism
chemical_list: D054405:Chemokine CCL3; D054357:Chemokine CXCL10; D054377:Chemokine CXCL12; D058534:Class I Phosphatidylinositol 3-Kinases; D019869:Phosphatidylinositol 3-Kinases; D043562:Receptors, G-Protein-Coupled; C496534:Ccl3 protein, mouse; C485433:Cxcl10 protein, mouse; C421683:Pik3cd protein, mouse