title: Taurine supplementation prevents ethanol-induced decrease in serum adiponectin and reduces hepatic steatosis in rats.
abstract: Chronic ethanol feeding decreases expression of adiponectin by adipocytes and circulating adiponectin. Adiponectin treatment during chronic ethanol feeding prevents liver injury in mice. Chronic ethanol feeding also increases oxidative and endoplasmic reticulum (ER) stress in adipose tissue. Here we tested the hypothesis that supplemental taurine, an amino acid that functions as a chemical chaperone/osmolyte and enhances cellular antioxidant activity, would prevent ethanol-induced decreases in adiponectin expression and attenuate liver injury. Serum adiponectin concentrations decreased as early as 4 to 7 days after feeding rats a 36% ethanol diet. This rapid decrease was associated with increased oxidative, but not ER, stress in subcutaneous adipose tissue. Taurine prevented ethanol-induced oxidative stress and increased inflammatory cytokine expression in adipose tissue. Ethanol feeding also rapidly decreased expression of transcription factors regulating adiponectin expression (CCAAT/enhancer binding protein alpha; peroxisome proliferator-activated receptor alpha/gamma) in subcutaneous adipose tissue. Taurine prevented the ethanol-induced decrease in CCAAT/enhancer binding protein alpha and peroxisome proliferator-activated receptor alpha, normalizing adiponectin messenger (m)RNA and serum adiponectin concentrations. In the liver, taurine prevented ethanol-induced oxidative stress and attenuated tumor necrosis factor alpha expression and steatosis, at least in part, by increasing expression of genes involved in fatty acid oxidation.
  
  
  CONCLUSIONS
  In subcutaneous adipose tissue, taurine decreased ethanol-induced oxidative stress and cytokine expression, as well as normalized expression of adiponectin mRNA. Taurine prevented ethanol-induced decreases in serum adiponectin; normalized adiponectin was associated with a reduction in hepatic oxidative stress, tumor necrosis factor alpha expression, and steatosis. Taken together, these data demonstrate that taurine has important protective effects against ethanol-induced tissue injury in both adipose and liver tissue.
authors.affiliation: Department of Nutrition, Case Western Reserve University, Cleveland, OH, USA.
authors.forename: Xiaocong
authors.initials: X
authors.lastname: Chen
authors.forename: Becky M
authors.initials: BM
authors.lastname: Sebastian
authors.forename: Hui
authors.initials: H
authors.lastname: Tang
authors.forename: Megan M
authors.initials: MM
authors.lastname: McMullen
authors.forename: Armend
authors.initials: A
authors.lastname: Axhemi
authors.forename: Donald W
authors.initials: DW
authors.lastname: Jacobsen
authors.forename: Laura E
authors.initials: LE
authors.lastname: Nagy
mesh_terms: D052242:Adiponectin / Q000097:blood*; D000273:Adipose Tissue / Q000187:drug effects / Q000378:metabolism*; D000818:Animals; D016207:Cytokines / Q000378:metabolism*; D019587:Dietary Supplements; D000431:Ethanol / Q000378:metabolism* / Q000633:toxicity; D005234:Fatty Liver / Q000517:prevention & control*; D008099:Liver / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects; D051381:Rats; D013654:Taurine / Q000378:metabolism* / Q000627:therapeutic use; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D052242:Adiponectin; D016207:Cytokines; D014409:Tumor Necrosis Factor-alpha; D013654:Taurine; D000431:Ethanol