A computational approach for genome-wide mapping of splicing factor binding sites.
Alternative splicing is regulated by splicing factors that serve as positive or negative effectors, interacting with regulatory elements along exons and introns. Here we present a novel computational method for genome-wide mapping of splicing factor binding sites that considers both the genomic environment and the evolutionary conservation of the regulatory elements. The method was applied to study the regulation of different alternative splicing events, uncovering an interesting network of interactions among splicing factors.
Akerman
Martin
M
Department of Biology, The Technion, Israel Institute of Technology, Haifa, Israel. makerman@tx.technion.ac.il
David-Eden
Hilda
H
Pinter
Ron Y
RY
Mandel-Gutfreund
Yael
Y
D000465:Algorithms; D017398:Alternative Splicing / Q000235:genetics*; D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D002874:Chromosome Mapping; D019295:Computational Biology / Q000379:methods*; D005091:Exons / Q000235:genetics; D016678:Genome / Q000235:genetics*; D015894:Genome, Human / Q000235:genetics; D006801:Humans; D051379:Mice; D009928:Organ Specificity; D016601:RNA-Binding Proteins / Q000378:metabolism*; D015203:Reproducibility of Results
D016601:RNA-Binding Proteins