NMDA receptors interact with flotillin-1 and -2, lipid raft-associated proteins.
N-methyl-D-aspartate receptors (NMDARs) mediate excitatory synaptic transmission in the brain. Here we demonstrate interactions between the NR2A and NR2B subunits of NMDARs with flotillin-1 (flot-1), a lipid raft-associated protein. When mapped, analogous regions in the far distal C-termini of NR2A and NR2B mediate binding to flot-1, and the prohibitin homology domain of flot-1 contains binding sites for NR2A and NR2B. Although NR2B can also directly bind to flot-2 via a separate region of its distal C-terminus, NMDARs were significantly more colocalized with flot-1 than flot-2 in cultured hippocampal neurons. Overall, this study defines a novel interaction between NMDARs and flotillins.
Swanwick
Catherine Croft
CC
Laboratory of Neurochemistry, National Institute on Deafness and Other Communication Disorders, National Institutes of Health, Bethesda, MD 20892, USA. swanwickc@mail.nih.gov
Shapiro
Marietta E
ME
Yi
Zhaohong
Z
Chang
Kai
K
Wenthold
Robert J
RJ
D000818:Animals; D007150:Immunohistochemistry; D008565:Membrane Proteins / Q000378:metabolism*; D011485:Protein Binding; D051381:Rats; D017207:Rats, Sprague-Dawley; D016194:Receptors, N-Methyl-D-Aspartate / Q000378:metabolism*; D013347:Subcellular Fractions / Q000378:metabolism
D008565:Membrane Proteins; D016194:Receptors, N-Methyl-D-Aspartate; C106354:flotillins