[Effect of angiotensin II type 1 receptor and angiotensin-converting enzyme gene silencing on nuclear factor-kappaB activity in hepatic stellate cells].
OBJECTIVE
To investigate the effect of angiotensin II (AngII) type 1 (AT-1) receptor and angiotensin-converting enzyme (ACE) gene silencing on nuclear factor-kappaB (NF-kappaB) activity in hepatic stellate cells (HSCs).


METHODS
pSilencer/AT-1 alpha receptor siRNA and pSilencer/ACE siRNA plasmids were transfected into cultured HSC-T6 cells, which were subsequently stimulated by 10(-6) mol/L AngII or ACE inhibitor (ACEI). The DNA binding activity of NF-kappaB in the transfected cells was analyzed using electrophoretic gel mobility shift assay (EMSA).


RESULTS
s Gel shift studies showed that stimulation of the HSCs by AngII markedly increased the DNA-binding activity of NF-kappaB, which was inhibited by the transfection with pSilencer/ AT-1 alpha receptor siRNA plasmid or pSilencer/ACE siRNA plasmid.


CONCLUSIONS
AT-1 alpha receptor and ACE gene silencing result in inhibition of NF-kappaB activity in HSCs in vitro.
Li
Xu
X
Department of Emergency Medicine, Nanfang Hospital, Southern Medical University, Guangzhou, China. mylx99@163.com
Zhang
Yi-Jun
YJ
Meng
Ying
Y
Zhou
Gao-Su
GS
Zhang
Zhen-Shu
ZS
D002460:Cell Line; D055166:Hepatic Stellate Cells / Q000166:cytology / Q000378:metabolism*; D006801:Humans; D016328:NF-kappa B / Q000235:genetics / Q000378:metabolism*; D007703:Peptidyl-Dipeptidase A / Q000235:genetics*; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics*; D044140:Receptor, Angiotensin, Type 1 / Q000235:genetics*; D014162:Transfection
C544065:AGTR1 protein, human; D016328:NF-kappa B; D034741:RNA, Small Interfering; D044140:Receptor, Angiotensin, Type 1; D007703:Peptidyl-Dipeptidase A