title: Label-free detection of B. anthracis spores using a surface plasmon resonance biosensor.
abstract: This study demonstrates the first use of surface plasmon resonance (SPR) technology for the rapid, sensitive and label-free detection of whole B. anthracis spores. The approach involves the use of an SPR biosensor (Biacore 3000), and a monoclonal antibody which was raised against the B. anthracis spore (mAb 8G3). By means of subtractive inhibition assays, whole B. anthracis spores with concentrations as low as 10(4) colony-forming units (CFU) ml(-1) can be detected within 40 min, and other related Bacillus spores, even in high concentrations, can be differentiated from B. anthracis spores.
authors.affiliation: State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, 430071, China.
authors.forename: Dian-Bing
authors.initials: DB
authors.lastname: Wang
authors.forename: Li-Jun
authors.initials: LJ
authors.lastname: Bi
authors.forename: Zhi-Ping
authors.initials: ZP
authors.lastname: Zhang
authors.forename: Yuan-Yuan
authors.initials: YY
authors.lastname: Chen
authors.forename: Rui-Fu
authors.initials: RF
authors.lastname: Yang
authors.forename: Hong-Ping
authors.initials: HP
authors.lastname: Wei
authors.forename: Ya-Feng
authors.initials: YF
authors.lastname: Zhou
authors.forename: Xian-En
authors.initials: XE
authors.lastname: Zhang
mesh_terms: D000911:Antibodies, Monoclonal / Q000737:chemistry; D000942:Antigens, Bacterial / Q000737:chemistry; D001408:Bacillus anthracis / Q000502:physiology*; D013171:Spores, Bacterial; D020349:Surface Plasmon Resonance / Q000379:methods*
chemical_list: D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial