title: Synergistic interactions between cefalexin and kanamycin in Mueller-Hinton broth medium and in milk.
abstract: OBJECTIVE
  This study investigated the in vitro bactericidal activity of an intramammary drug product by comparing the kill kinetics of cefalexin and kanamycin, alone and in fixed ratio combination, against Streptococcus uberis, Staphylococcus aureus and Escherichia coli strains isolated from field cases of bovine mastitis. The effect of milk as a diluent on the rate of bacterial killing was also assessed.
  
  
  RESULTS
  Antibacterial kill kinetics was determined against each bacterial strain in Mueller-Hinton broth (MHB) and in milk. In MHB, the fixed cefalexin : kanamycin combination (1.5 : 1 w/w) exhibited a clear synergistic bactericidal activity against the strains tested. The combination also showed an enhanced killing activity in milk, as compared to either agent alone.
  
  
  CONCLUSIONS
  The data show the occurrence of synergistic interactions between cefalexin and kanamycin, resulting in a faster and enhanced bactericidal activity against major mastitis pathogens.
  
  
  CONCLUSIONS
  The study demonstrated that the combination exhibited a larger and faster rate of kill of S. aureus, S. uberis and E. coli compared to either cefalexin or kanamycin alone, while using a lower total amount of antibiotic. Synergistic and additive effects were also observed when milk was used as a medium. The results support the use of this combination of narrow spectrum antibiotics to treat clinical mastitis via the intramammary route and provide data on its killing kinetics.
authors.affiliation: Unité de Pathologie Infectieuse, Ecole Nationale Vétérinaire, Nantes Cedex, France. ganiere@vet-nantes.fr
authors.forename: J P
authors.initials: JP
authors.lastname: Ganière
authors.forename: L
authors.initials: L
authors.lastname: Denuault
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001419:Bacteria / Q000187:drug effects*; D002417:Cattle; D002506:Cephalexin / Q000494:pharmacology*; D015169:Colony Count, Microbial; D004357:Drug Synergism; D004926:Escherichia coli / Q000187:drug effects; D005260:Female; D007612:Kanamycin / Q000494:pharmacology*; D008414:Mastitis, Bovine / Q000382:microbiology*; D008826:Microbial Sensitivity Tests; D008892:Milk / Q000737:chemistry; D013211:Staphylococcus aureus / Q000187:drug effects; D013291:Streptococcus / Q000187:drug effects; D013997:Time Factors
chemical_list: D000900:Anti-Bacterial Agents; D007612:Kanamycin; D002506:Cephalexin