title: Phase assemblage study and cytocompatibility property of heat treated potassium magnesium phosphate-silicate ceramics.
abstract: This article reports the study on a new generation bioactive ceramic, based on MgKPO(4) (Magnesium Potassium Phosphate, abbreviated as MKP) for biomedical applications. A series of heat treatment experiments on the slip cast silica (SiO(2)) containing MKP ceramics were carried out at 900, 1,000 and 1,100 degrees C for 4 h in air. The density of the slip cast ceramic increases to 2.5 gm/cm(3) upon heat treatment at 900 degrees C. However, no significant change in density is measured upon heat treatment to higher temperature of 1,000 and 1,100 degrees C. On the basis of XRD results, the presence of K(2)MgSi(5)O(12) and dehydrated MgKPO(4) were confirmed and complementary information has also been obtained using FT-IR and Raman spectroscopy. In order to confirm the in vitro cytocompatibility property, the cell culture tests were carried out on selected samples and the results reveal good cell adhesion and spreading of L929 mouse fibroblast cells. MTT assay analysis with L929 cells confirmed non-cytotoxic behavior of MKP containing ceramics and the results are comparable with sintered HAp ceramics. It is expected that the newly developed MKP based materials could be a good substitute for hydroxyapatite (HAp or HA) based bioceramics.
authors.affiliation: Laboratory for Advanced Ceramics, Department Materials and Metallurgical Engineering, Indian Institute of Technology Kanpur, Kanpur, UP, India.
authors.forename: Ravi
authors.initials: R
authors.lastname: Kumar
authors.forename: Sushma
authors.initials: S
authors.lastname: Kalmodia
authors.forename: Shekhar
authors.initials: S
authors.lastname: Nath
authors.forename: Dileep
authors.initials: D
authors.lastname: Singh
authors.forename: Bikramjit
authors.initials: B
authors.lastname: Basu
mesh_terms: D000818:Animals; D002448:Cell Adhesion / Q000502:physiology; D002470:Cell Survival / Q000502:physiology; D002478:Cells, Cultured; D002516:Ceramics / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology; D005347:Fibroblasts / Q000502:physiology; D006358:Hot Temperature; D017616:Magnesium Compounds / Q000737:chemistry*; D008422:Materials Testing; D051379:Mice; D044367:Phase Transition; D010710:Phosphates / Q000737:chemistry*; D017680:Potassium Compounds / Q000737:chemistry*; D017640:Silicates / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology; D017550:Spectroscopy, Fourier Transform Infrared; D013059:Spectrum Analysis, Raman; D014961:X-Ray Diffraction
chemical_list: D017616:Magnesium Compounds; D010710:Phosphates; D017680:Potassium Compounds; D017640:Silicates; C030781:magnesium phosphate; C013216:potassium phosphate