Benzoate-mediated changes on expression profile of soluble proteins in Serratia sp. DS001.
OBJECTIVE
To assess differences in protein expression profile associated with shift in carbon source from succinate to benzoate in Serratia sp. DS001 using a proteomics approach.


RESULTS
A basic proteome map was generated for the soluble proteins extracted from Serratia sp. DS001 grown in succinate and benzoate. The differently and differentially expressed proteins were identified using ImageMaster 2D Platinum software (GE Healthcare). The identity of the proteins was determined by employing MS or MS/MS. Important enzymes such as Catechol 1,2 dioxygenase and transcriptional regulators that belong to the LysR superfamily were identified.


CONCLUSIONS
Nearly 70 proteins were found to be differentially expressed when benzoate was used as carbon source. Based on the protein identity and degradation products generated from benzoate it is found that ortho pathway is operational in Serratia sp. DS001.


CONCLUSIONS
Expression profile of the soluble proteins associated with shift in carbon source was mapped. The study also elucidates degradation pathway of benzoate in Serratia sp. DS001 by correlating the proteomics data with the catabolites of benzoate.
Pandeeti
E V P
EV
Department of Animal Sciences, School of Life Sciences, University of Hyderabad, Hyderabad, India.
Chinnaboina
M R
MR
Siddavattam
D
D
D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D001565:Benzoates / Q000378:metabolism*; D020869:Gene Expression Profiling; D015964:Gene Expression Regulation, Bacterial; D008969:Molecular Sequence Data; D040901:Proteomics; D012705:Serratia / Q000235:genetics* / Q000378:metabolism*; D019802:Succinic Acid / Q000378:metabolism
D001426:Bacterial Proteins; D001565:Benzoates; D019802:Succinic Acid