title: KCNQ1 loss-of-function mutation impairs gastric acid secretion in mice.
abstract: The KCNQ1 channel is abundantly expressed in the gastric parietal cells. Although the functional coupling of KCNQ1 with the H(+)/K(+)-ATPase has already been confirmed on the basis of pharmacological kinetics, the effect of a KCNQ1 loss-of-function mutation on gastric acidification remains unclear. In this study, parietal cells and gastric glands from both C57BL/6 J mice (normal control) and J343 mice (mice with a KCNQ1 loss-of-function mutation) were isolated to study the effects of KCNQ1 on gastric acidification. We found that the mutation limited intracellular acidification of parietal cells and H(+) secretion of the stomach in response to histamine. Thus, a KCNQ1 loss-of-function mutation may impair gastric acid secretion.
authors.affiliation: Department of Cardiology, Tongji Hospital, Tongji University School of Medicine, Shanghai, China.
authors.forename: Qin
authors.initials: Q
authors.lastname: Pan
authors.forename: Jun
authors.initials: J
authors.lastname: Ma
authors.forename: Qinshu
authors.initials: Q
authors.lastname: Zhou
authors.forename: Jun
authors.initials: J
authors.lastname: Li
authors.forename: Yongqing
authors.initials: Y
authors.lastname: Tang
authors.forename: Yi
authors.initials: Y
authors.lastname: Liu
authors.forename: Yiqing
authors.initials: Y
authors.lastname: Yang
authors.forename: Junjie
authors.initials: J
authors.lastname: Xiao
authors.forename: Luying
authors.initials: L
authors.lastname: Peng
authors.forename: Pengjuan
authors.initials: P
authors.lastname: Li
authors.forename: Dandan
authors.initials: D
authors.lastname: Liang
authors.forename: Hong
authors.initials: H
authors.lastname: Zhang
authors.forename: Yi-Han
authors.initials: YH
authors.lastname: Chen
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D005744:Gastric Acid / Q000378:metabolism*; D017506:H(+)-K(+)-Exchanging ATPase / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D051657:KCNQ1 Potassium Channel / Q000235:genetics* / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D009154:Mutation / Q000235:genetics; D010295:Parietal Cells, Gastric / Q000378:metabolism*
chemical_list: D051657:KCNQ1 Potassium Channel; C496910:Kcnq1 protein, mouse; D017506:H(+)-K(+)-Exchanging ATPase