The proteome of mesenteric lymph during acute pancreatitis and implications for treatment.
BACKGROUND
The protein fraction of mesenteric lymph during acute pancreatitis and other critical illness is thought to contain toxic factors. However, we do not have a complete description of the mesenteric lymph proteome during acute pancreatitis.


OBJECTIVE
The aim of this study was to define the proteomic changes in mesenteric lymph during acute pancreatitis.


METHODS
Animal Laboratory, University of Auckland, New Zealand.


METHODS
Mesenteric lymph was collected from sixteen male Wistar rats randomised to Group 1 (n=8) with taurocholate induced acute pancreatitis and Group 2 (n=8) sham control. The lymph was subjected to proteomic analysis using iTRAQ (Applied Biosystems, Foster City, CA, USA) and liquid chromatography-tandem mass spectrometry.


RESULTS
Two hundred and forty-five proteins including 35 hypothetical proteins were identified in mesenteric lymph. Eight of the 245 proteins had a significant increase in their relative abundance in acute pancreatitis conditioned mesenteric lymph, and 7 of these were pancreatic catabolic enzymes (pancreatic amylase 2, pancreatic lipase, carboxypeptidase A2, chymotrypsinogen B, carboxypeptidase B1, cationic trypsinogen, ribonuclease 1).


CONCLUSIONS
This is the first comprehensive description of the proteome of mesenteric lymph during acute pancreatitis and has demonstrated a significantly increased relative abundance of 7 secreted pancreatic catabolic enzymes in acute pancreatitis conditioned mesenteric lymph. This study provides a clear rationale for further research to investigate the efficacy of enteral protease inhibitors in the treatment of acute pancreatitis.
Mittal
Anubhav
A
Department of Surgery, Faculty of Medicine and Health Sciences, University of Auckland, Auckland, New Zealand.
Phillips
Anthony R J
AR
Middleditch
Martin
M
Ruggiero
Katya
K
Loveday
Benjamin
B
Delahunt
Brett
B
Cooper
Garth J S
GJ
Windsor
John A
JA
D000208:Acute Disease; D000818:Animals; D002853:Chromatography, Liquid / Q000379:methods; D030562:Databases, Protein; D008196:Lymph / Q000378:metabolism*; D008297:Male; D008643:Mesentery; D010195:Pancreatitis / Q000139:chemically induced / Q000378:metabolism* / Q000628:therapy; D020543:Proteome / Q000032:analysis*; D040901:Proteomics / Q000379:methods*; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D015203:Reproducibility of Results; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D013656:Taurocholic Acid
D020543:Proteome; D013656:Taurocholic Acid