Elevated plasma stromal cell-derived factor 1 protein and its gene polymorphism in patients with pelvic inflammatory disease.
The objective was to compare the expression of plasma stromal cell-derived factor 1 and the gene polymorphism in patients with pelvic inflammatory disease and healthy controls. The enzyme-linked immunosorbent assay and polymerase chain reaction-restriction fragment length polymorphism were, respectively, used to measure the plasma stromal cell-derived factor 1alpha level and stromal cell-derived factor 1 polymorphism in 50 healthy controls and in 44 patients with pelvic inflammatory disease before and after they received routine treatment protocols. The level of plasma stromal cell-derived factor 1alpha was elevated in patients with pelvic inflammatory disease compared to normal controls and decreased significantly after treatment. There were significant correlations between plasma stromal cell-derived factor 1alpha level and neutrophil count as well as between stromal cell-derived factor 1alpha level and white blood cell count in patients with pelvic inflammatory disease. There was no significantly different distribution of stromal cell-derived factor 1 genotypes between patients with pelvic inflammatory disease and normal controls. Patients with pelvic inflammatory disease having stromal cell-derived factor 1-3'A allele were associated with significantly elevated plasma stromal cell-derived factor 1alpha concentration compared to patients with pelvic inflammatory disease having G/G homozygous alleles (P < .02). In normal controls, there was no significant difference in the plasma stromal cell-derived factor 1 level between individuals with and without stromal cell-derived factor 1-3'A allele. When the cutoff level of plasma stromal cell-derived factor 1alpha level was determined to be 2192 pg/mL based on receiver-operating characteristic curve, the sensitivity, specificity, positive predictive value, and negative predictive value as well as accuracy were 77.3%, 88.0%, 85.0%, 81.5%, and 83.0%. In conclusion, when the cutoff level was determined to be 2192 pg/mL, plasma stromal cell-derived factor 1alpha level can be used to predict pelvic inflammatory disease.
Tsai
Hsiu-Ting
HT
School of Nursing, College of Nursing, Chung Shan Medical University, Taichung, Taiwan.
Tee
Yi-Torng
YT
Hsieh
Yi-Hsien
YH
Chiou
Hui-Ling
HL
Lin
Chiao-Wen
CW
Tsai
Hsiu-Chen
HC
Wang
Po-Hui
PH
Yang
Shun-Fa
SF
D000328:Adult; D015415:Biomarkers / Q000097:blood; D016022:Case-Control Studies; D054377:Chemokine CXCL12 / Q000096:biosynthesis / Q000097:blood* / Q000235:genetics*; D005260:Female; D006801:Humans; D000292:Pelvic Inflammatory Disease / Q000097:blood* / Q000175:diagnosis / Q000235:genetics*; D011110:Polymorphism, Genetic; D011237:Predictive Value of Tests
D015415:Biomarkers; C515255:CXCL12 protein, human; D054377:Chemokine CXCL12