title: Shotgun mass spectrometry analysis of the human thalamus proteome.
abstract: The thalamus plays pivotal roles in the central nervous system as relay center for organizing information, such as auditory and visual senses from diverse brain regions and their re-distribution to the cerebral cortex. Brain diseases including schizophrenia, Parkinson's disease, epilepsy, and bipolar disorder have been associated with the thalamus. We performed a shotgun proteome analysis of iTRAQ-labeled tryptic peptides of human mediodorsal thalamus protein extracts coming from two healthy male and two healthy female subjects. The shotgun workflow consisted of IEF fractionation, RP LC and MALDI-TOF/TOF mass spectrometric analysis. We were able to identify 542 proteins that are involved in different biological processes and from diverse cellular localizations. A considerable fraction of these proteins had not been identified by traditional proteomics methods such as 2-DE. The thalamus proteome contributes to the knowledge of the human brain proteome and future applications in basic and clinical research.
authors.affiliation: Max Planck Institute of Psychiatry, Munich, Germany. martins@mpipsykl.mpg.de
authors.forename: Daniel
authors.initials: D
authors.lastname: Martins-de-Souza
authors.forename: Giuseppina
authors.initials: G
authors.lastname: Maccarrone
authors.forename: Stefan
authors.initials: S
authors.lastname: Reckow
authors.forename: Peter
authors.initials: P
authors.lastname: Falkai
authors.forename: Andrea
authors.initials: A
authors.lastname: Schmitt
authors.forename: Christoph W
authors.initials: CW
authors.lastname: Turck
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D005260:Female; D006801:Humans; D008297:Male; D013058:Mass Spectrometry / Q000379:methods*; D008875:Middle Aged; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000737:chemistry* / Q000378:metabolism; D010455:Peptides / Q000737:chemistry* / Q000378:metabolism; D020543:Proteome / Q000032:analysis*; D040901:Proteomics / Q000379:methods; D013788:Thalamus / Q000737:chemistry*; D014357:Trypsin / Q000378:metabolism
chemical_list: D009419:Nerve Tissue Proteins; D010455:Peptides; D020543:Proteome; D014357:Trypsin