title: Genetic and epidemiological characterization of Stretch Lagoon orbivirus, a novel orbivirus isolated from Culex and Aedes mosquitoes in northern Australia.
abstract: Stretch Lagoon orbivirus (SLOV) was isolated in 2002 from pooled Culex annulirostris mosquitoes collected at Stretch Lagoon, near the Wolfe Creek national park in the Kimberley region of Western Australia. Conventional serological tests were unable to identify the isolate, and electron microscopy indicated a virus of the genus Orbivirus, family Reoviridae. Here, a cDNA subtraction method was used to obtain approximately one-third of the viral genome, and further sequencing was performed to complete the sequences of segment 1 (viral polymerase) and segment 2 (conserved inner-core protein). Phylogenetic analysis showed that SLOV should be considered a new species within the genus Orbivirus. A real-time RT-PCR test was designed to study the epidemiology of SLOV in the field. Six additional isolates of SLOV were identified, including isolates from four additional locations and two additional mosquito species. Horses, donkeys and goats were implicated as potential vertebrate hosts in a serological survey.
authors.affiliation: CSIRO Livestock Industries, Australian Animal Health Laboratory, East Geelong, VIC 3220, Australia.
authors.forename: Chris
authors.initials: C
authors.lastname: Cowled
authors.affiliation: Center for Infection and Immunity, Mailman School of Public Health, Columbia University, New York, NY, USA.
authors.forename: Gustavo
authors.initials: G
authors.lastname: Palacios
authors.affiliation: Northern Territory Department of Primary Industries, Fisheries and Mines, Berrimah Veterinary Laboratories, Berrimah, Northern Territory 0801, Australia.
authors.forename: Lorna
authors.initials: L
authors.lastname: Melville
authors.affiliation: Northern Territory Department of Primary Industries, Fisheries and Mines, Berrimah Veterinary Laboratories, Berrimah, Northern Territory 0801, Australia.
authors.forename: Richard
authors.initials: R
authors.lastname: Weir
authors.affiliation: Northern Territory Department of Primary Industries, Fisheries and Mines, Berrimah Veterinary Laboratories, Berrimah, Northern Territory 0801, Australia.
authors.forename: Susan
authors.initials: S
authors.lastname: Walsh
authors.affiliation: Northern Territory Department of Primary Industries, Fisheries and Mines, Berrimah Veterinary Laboratories, Berrimah, Northern Territory 0801, Australia.
authors.forename: Steven
authors.initials: S
authors.lastname: Davis
authors.affiliation: CSIRO Livestock Industries, Australian Animal Health Laboratory, East Geelong, VIC 3220, Australia.
authors.forename: Aneta
authors.initials: A
authors.lastname: Gubala
authors.affiliation: Center for Infection and Immunity, Mailman School of Public Health, Columbia University, New York, NY, USA.
authors.forename: W Ian
authors.initials: WI
authors.lastname: Lipkin
authors.affiliation: Center for Infection and Immunity, Mailman School of Public Health, Columbia University, New York, NY, USA.
authors.forename: Thomas
authors.initials: T
authors.lastname: Briese
authors.affiliation: CSIRO Livestock Industries, Australian Animal Health Laboratory, East Geelong, VIC 3220, Australia.
authors.forename: David
authors.initials: D
authors.lastname: Boyle
mesh_terms: D000330:Aedes / Q000821:virology*; D000818:Animals; D001315:Australia; D001483:Base Sequence; D002460:Cell Line; D016000:Cluster Analysis; D006224:Cricetinae; D003465:Culex / Q000821:virology*; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D008969:Molecular Sequence Data; D018163:Orbivirus / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D010802:Phylogeny; D012367:RNA, Viral / Q000235:genetics*; D012088:Reoviridae Infections / Q000453:epidemiology / Q000662:veterinary*; D017422:Sequence Analysis, DNA; D017385:Sequence Homology
chemical_list: D018076:DNA, Complementary; D012367:RNA, Viral