[Construction of eukaryotic expression vectors of basic fibroblast growth factor and transfection in rabbit bone marrow stromal cells].
OBJECTIVE
To investigate approach and possibility of transferring basic fibroblast growth factor (bFGF) gene into rabbit bone marrow stromal cells (BMSCs).


METHODS
The eukaryotic expression vectors harboring bFGF cDNA were constructed and transfected into rabbit BMSCs mediated by liposome. The transcription and expression of bFGF gene in the transfected BMSCs were detected by means of morphological observation, immunohistochemistry, enzyme-linked immunosorbent assay (ELISA) and RT-PCR. The changes in the biological characteristics of the transfected MSCs were also observed.


RESULTS
Stable overexpression of bFGF protein was detected in the transfected BMSCs, which showed differentiation towards chondrocyte lineage.


CONCLUSIONS
Stable expression of bFGF gene in transfected BMSCs can induce cell differentiation into chondrocyte lineage.
Xu
Zhong-Shi
ZS
Department of Orthopedics, Shenzhen People's Hospital, Shenzhen, China.
Xiao
De-Ming
DM
Lin
Bo-Wen
BW
Lu
Xiao-Hu
XH
Li
Ran
R
D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology / Q000378:metabolism*; D005260:Female; D016222:Fibroblast Growth Factor 2 / Q000096:biosynthesis / Q000235:genetics*; D015870:Gene Expression; D005822:Genetic Vectors / Q000235:genetics; D008297:Male; D011817:Rabbits; D017154:Stromal Cells / Q000166:cytology / Q000378:metabolism*; D014162:Transfection
D016222:Fibroblast Growth Factor 2