Rapid multi-residue analysis of antibiotics in muscle by liquid chromatography-tandem mass spectrometry.
A way of carrying out simple and rapid multi-residue analysis of antibiotics in porcine and bovine muscle by liquid chromatography-tandem mass spectrometry (LC-MS/MS) is described. The method has previously been published as a screening method, but the scope has now been extended to quantification and confirmation. Nineteen compounds from five different classes of antibiotics, i.e. tetracyclines, sulfonamides, quinolones, (-lactams and macrolides, are included in the method. The samples are extracted by a single extraction using 70% methanol, diluted with water and injected in the LC-MS/MS. The run time is 7min per injection. About 60 samples can be analysed in 24h. By using this method the need for a separate screening step prior to confirmation is eliminated and consequently the total time from sampling to a confirmed result will be considerably reduced. Validation was performed according to Commission Decision 2002/657/EC.
Granelli
K
K
Chemistry Division 1, National Food Administration, Box 622, SE-751 26 Uppsala, Sweden.
Elgerud
Christina
C
Lundström
Asa
A
Ohlsson
Anita
A
Sjöberg
Pernilla
P
D000818:Animals; D000900:Anti-Bacterial Agents / Q000032:analysis* / Q000302:isolation & purification; D002417:Cattle; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004350:Drug Residues / Q000032:analysis* / Q000302:isolation & purification; D009132:Muscles / Q000737:chemistry*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D013552:Swine; D053719:Tandem Mass Spectrometry
D000900:Anti-Bacterial Agents