[Expression of Smad4 in prostate cancer LNCaP and ARCaP cell lines with different metastatic potentials and its significance].
OBJECTIVE
To observe the expression of Smad4, the core of TGF-beta/Smads signal transduction pathway in different prostate cancer cell lines, and explore their molecular mechanism of bone metastatic potential.


METHODS
The Millicell polycarbonate filter coated with matrigel was used to confirm the invasive potency of LNCaP and ARCaP cell lines (IF11 and IA8). The expressions of the Smad4 protein and mRNA in these prostate cancer cells with different metastatic potentials were detected by Western blotting and RT-PCR, respectively.


RESULTS
ARCaP cell lines (IF11 and IA8) exhibited a stronger potency of invasion than LNCaP (P < 0.01). The Smad4 protein and mRNA highly expressed in the LNCaP cell line that was well-known with a low metastatic potential, but not in the ARCaP (IF11 or IA8) cells with high metastatic potentials (P < 0.01).


CONCLUSIONS
Smad4 expresses differently in LNCaP and ARCaP cell lines with different metastatic potentials and, as a tumor suppressive gene, its deficient expression may play an important role in the invasion and metastasis of advanced prostate cancer.
Wu
Kai-Jie
KJ
Department of Urology, The First Affiliated Hospital, Xi'an Jiaotong University School of Medicine, Xi'an, Shaanxi 710061, China.
Zhang
Dong
D
Zhu
Guang-Feng
GF
Cheng
He-Peng
HP
Zeng
Jin
J
Zhang
Lin-Lin
LL
Wang
Xin-Yang
XY
He
Da-Lin
DL
D045744:Cell Line, Tumor; D006801:Humans; D008297:Male; D009362:Neoplasm Metastasis; D011471:Prostatic Neoplasms / Q000378:metabolism* / Q000473:pathology*; D012333:RNA, Messenger / Q000235:genetics; D051901:Smad4 Protein / Q000096:biosynthesis*; D016212:Transforming Growth Factor beta / Q000378:metabolism
D012333:RNA, Messenger; C497796:SMAD4 protein, human; D051901:Smad4 Protein; D016212:Transforming Growth Factor beta