A novel chemiluminescent flow injection analysis of trace amounts of rutin by its inhibition of the luminol-hydrogen peroxide reaction catalyzed by tetrasulfonated colbalt phthalocyanine.
Based on the inhibition effect of rutin on the luminol-hydrogen peroxide chemiluminescence (CL) system catalyzed by tetrasulfonated colbalt phthalocyanine (CoTSPc), a sensitive flow-injection CL method has been developed for the determination of rutin. The CL reaction mechanism was carefully investigated by examining CL emission spectra, UV-visible spectra and variation of reaction conditions. It was found that there existed a linear relationship between CL intensity and the concentration of rutin in the range of 8.0 x 10(-9) to 1.0 x 10(-6) mol L(-1), and the detection limit is 3.8 x 10(-9) mol L(-1). This proposed method is sensitive, convenient and simple, and has been applied to the determination of rutin in commercial rutin tablets with satisfactory results.
Zhu
Qiyong
Q
Department of Chemistry, Huainan Normal University, Huainan 232001, People's Republic of China. zhuqiyong6062@yahoo.com.cn
D002384:Catalysis; D003035:Cobalt / Q000737:chemistry*; D006861:Hydrogen Peroxide / Q000737:chemistry*; D007211:Indoles / Q000737:chemistry*; D054833:Isoindoles; D049408:Luminescent Agents / Q000737:chemistry; D008163:Luminescent Measurements / Q000379:methods*; D008165:Luminol / Q000737:chemistry*; D015394:Molecular Structure; D012431:Rutin / Q000032:analysis* / Q000737:chemistry; D013455:Sulfur / Q000737:chemistry*; D013607:Tablets / Q000032:analysis / Q000737:chemistry
D007211:Indoles; D054833:Isoindoles; D049408:Luminescent Agents; D013607:Tablets; D003035:Cobalt; D008165:Luminol; D012431:Rutin; D013455:Sulfur; D006861:Hydrogen Peroxide; C013647:phthalocyanine