[Mass chromatographic analysis on different protein expression of human periodontal ligament cell under static pressure].
OBJECTIVE
To analyze the protein expression of human periodontal ligament cells (HPDLCs) under static pressure.


METHODS
The proteins of two groups of human periodontal ligament cell (one group was interfered by static pressure and one group was not) were analyzed by two-dimensional electrophoresis and mass chromatographic analysis. The silver stained proteins spots were analyzed by Image Master 2D Platinum Software 5.0. There were about 720 and 730 detectable spots on the two 2D-gels separately and nearly 30 spots which were differentially expressed.


RESULTS
With direct MALDI-TOF mass spectrometry and protein database searching, 2 protein spots out of 5 new appeared spots were identified. Presenilin 2 and catechol O-methyltransferase were expressed only in the group which was interfered by static pressure.


CONCLUSIONS
The results of mass chromatographic analysis demonstrate that Notch pathway and calcitonin gene-related peptide(CGRP) may be the partners of the channel of biomechanical signal conduction of the periodontal ligament cell.
An
Yuan-Yuan
YY
Department of Orthodontics, Medical School of Xi'an Jiaotong University, Xi'an 710004, China. ann@stu.xjtu.edu.cn
Zhou
Hong
H
Ruan
Yu-Song
YS
Rao
Guo-Zhou
GZ
D015180:Electrophoresis, Gel, Two-Dimensional; D006801:Humans; D010513:Periodontal Ligament; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization