title: Microbial conversion of glucose to a novel chemical building block, 2-pyrone-4,6-dicarboxylic acid.
abstract: 2-Pyrone-4,6-dicarboxylic acid (PDC) is a catabolic intermediate in Sphingobium sp. SYK-6 (previously characterized as Sphingomonas paucimobilis SYK-6), which is a degrader of lignin-derived aromatic compounds. Recently, PDC has been also characterized as a novel starting material for several potentially useful synthetic polymers. In a previous study, we constructed a biosynthetic system in which PDC was generated efficiently from a chemically synthesized compound, protocatechuate. In order to develop an alternative system for production of PDC, we tried to generate it from glucose, which is a low-cost sugar that can be obtained from abundant cellulosic wastes and biomass crops. We designed a metabolic bypass to PDC from the shikimate pathway in recombinant Escherichia coli cells. PDC accumulated in the medium of recombinant E. coli cells that had been transformed with genes isolated from Emericella niger, E. coli, Pseudomonas putida, and Sphingobium sp. SYK-6. The yield of PDC depended on the combination of genes that we introduced into the cells and on the specific of host strain. Under optimal conditions, the yield and titer of PDC were, respectively, 17.3% and 0.35 mg/l when the concentration of glucose was 2 g/l and the culture volume was 50 ml. Our results open up the possibility of novel utilization of biomass as the source of a useful chemical building block.
authors.affiliation: Graduate School of Bio-Applications and Systems Engineering, Tokyo University of Agriculture and Technology, Koganei, Tokyo 184-8588, Japan.
authors.forename: Masahiro
authors.initials: M
authors.lastname: Nakajima
authors.forename: Yukari
authors.initials: Y
authors.lastname: Nishino
authors.forename: Masatsugu
authors.initials: M
authors.lastname: Tamura
authors.forename: Kohei
authors.initials: K
authors.lastname: Mase
authors.forename: Eiji
authors.initials: E
authors.lastname: Masai
authors.forename: Yuichiro
authors.initials: Y
authors.lastname: Otsuka
authors.forename: Masaya
authors.initials: M
authors.lastname: Nakamura
authors.forename: Kanna
authors.initials: K
authors.lastname: Sato
authors.forename: Masao
authors.initials: M
authors.lastname: Fukuda
authors.forename: Kiyotaka
authors.initials: K
authors.lastname: Shigehara
authors.forename: Seiji
authors.initials: S
authors.lastname: Ohara
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Katayama
authors.forename: Shinya
authors.initials: S
authors.lastname: Kajita
mesh_terms: D001709:Biotechnology / Q000379:methods; D003470:Culture Media / Q000032:analysis; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D005947:Glucose / Q000235:genetics / Q000378:metabolism*; D009154:Mutation; D011753:Pyrones / Q000378:metabolism*; D012765:Shikimic Acid / Q000378:metabolism*; D020578:Sphingomonas / Q000235:genetics / Q000378:metabolism*
chemical_list: D003470:Culture Media; D011753:Pyrones; D012765:Shikimic Acid; C024153:alpha-pyrone-4,6-dicarboxylic acid; D005947:Glucose