Dielectric dispersion of suspended cells using 3D reconstructed morphology model.
In the framework of this study, novel method for dispersion analysis of cellular suspensions is presented. The method is fundamentally based on the ability to reconstruct the exact 3D morphology of a given cell with resolution accuracy of few nanometers using AFM imaging. By applying a reverse engineering approach, the morphology of the cell is constructed based on a set of measured spatial points that describes its geometry. The permittivity spectrum of the reconstructed cell is then directly derived based on computational solution of complex potential problem using 3D Boundary Element Method. The applicability of the method is demonstrated both theoretically and experimentally with tight comparison to the well known shell models. This comparison reveals significant deviations between the models, and hence, emphasises the vast effect of morphology in dispersion analysis of cellular suspensions.
Ron
Amit
A
Department of Physical Electronics, Faculty of Engineering, Tel-Aviv University, Israel. amitron@eng.tau.ac.il
Singh
Ragini Raj
RR
Fishelson
Nick
N
Shur
Irena
I
Socher
Rina
R
Croitoriu
Nathan
N
Benayahu
Dafna
D
Shacham-Diamand
Yosi
Y
D000465:Algorithms; D000818:Animals; D002460:Cell Line; D045744:Cell Line, Tumor; D048430:Cell Shape; D003198:Computer Simulation; D004553:Electric Conductivity; D006801:Humans; D018625:Microscopy, Atomic Force / Q000379:methods*; D008954:Models, Biological