A bioluminescent enzyme immunoassay for prostaglandin E(2) using Cypridina luciferase.
Prostaglandin E(2) is one of the major cyclooxygenase metabolites of arachidonic acid. We developed a competitive immunosorbent assay for prostaglandin E(2) utilizing a bioluminescent enzyme Cypridina luciferase. The prostaglandin E(2) amount could be quantified over the concentration ranging from 7.8 to 500 pg/mL. The amount of unlabeled prostaglandin E(2) required to displace 50% of the maximal binding of Cypridina luciferase-labeled prostaglandin E(2) (B/B(0)) was approximately 35 pg/mL. The results show a great potential of Cypridina luciferase as a new labeling enzyme for enzyme-linked immunosorbent assay.
Wu
Chun
C
Research Institute for Cell Engineering, National Institute of Advanced Industrial Science and Technology, Osaka 563-8577, Japan. chun-wu@aist.go.jp
Irie
Saki
S
Yamamoto
Shozo
S
Ohmiya
Yoshihiro
Y
D000818:Animals; D003530:Cyprinidae; D015232:Dinoprostone / Q000032:analysis*; D006801:Humans; D007124:Immunoenzyme Techniques / Q000379:methods*; D008156:Luciferases; D049408:Luminescent Agents; D008163:Luminescent Measurements
D049408:Luminescent Agents; D008156:Luciferases; D015232:Dinoprostone