pTOM, a vector for expressing two proteins in mammalian cells.
We developed a pTOM construct that can express two proteins in a cell. Using pTOM ensures that two polypeptide-encoding nucleotide sequences are simultaneously transfected into the same cell. The ability to simultaneously express two separate polypeptide-encoding nucleotide sequences from the same vector in the same cell allows the user to determine the relationship between the two proteins. Another advantage is that one of the proteins can be used as a transfection marker/selector. The vector contains multiple cloning sites for insertions of polypeptide-encoding nucleotide sequences. Positive clones can be easily selected when performing cloning using ampicillin. Overall, this vector provides a convenient way to express dual proteins in a single mammalian cell.
Lin
Yenshou
Y
Department of Life Science, National Taiwan Normal University, 88 Ting-Chou Road, Taipei 116, Taiwan. yenshoulin@ntnu.edu.tw
Avruch
Joseph
J
D002460:Cell Line; D003587:Cytomegalovirus / Q000235:genetics; D015870:Gene Expression / Q000235:genetics*; D005822:Genetic Vectors / Q000235:genetics*; D005982:Glutathione Transferase / Q000235:genetics; D049452:Green Fluorescent Proteins / Q000235:genetics; D006801:Humans; D008957:Models, Genetic; D020648:Peptide Elongation Factor 1 / Q000235:genetics; D010455:Peptides; D011401:Promoter Regions, Genetic
D020648:Peptide Elongation Factor 1; D010455:Peptides; D049452:Green Fluorescent Proteins; D005982:Glutathione Transferase