[Determination of zizybeoside II of Ziziphus jujuba by HPLC].
OBJECTIVE
To develop a quantitative method for determination of zizybeoside II in Ziziphus jujuba.


METHODS
The samples were separated at 30 degrees C on a Zorbax SB-C18 column eluted with methanol-water (20 : 80) as the mobile phase. Flow rate was set at 1.0 mL x min(-1) and the detection wavelength was set at 210 nm.


RESULTS
The calibration curve was linear within the range from 0.046 to 0.582 microg (r = 0.999 9) and the average recovery was 97.2%. 12 batches of the crude drugs purchased from different areas were determined and the contents of zizybeoside II in Fructus Jujubae were fluctuated from 0.013% to 0.041%.


CONCLUSIONS
The method is simple, repeatable and could be used for the quality control of Z. jujuba.
Niu
Ji-Wei
JW
College of Veterinary Medicine, Northwest Agricultural and Forest University, Yangling 712100, China.
Zhang
Qi-Wei
QW
Gong
Mu-Xin
MX
Yan
Li-Hua
LH
Zhu
Jing-Jing
JJ
Wang
Zhi-Min
ZM
Li
Qin-Fan
QF
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000302:isolation & purification; D012503:Saponins / Q000032:analysis*; D031957:Ziziphus / Q000737:chemistry*
D004365:Drugs, Chinese Herbal; D012503:Saponins