title: Integrated Raman spectroscopy and trimodal wide-field imaging techniques for real-time in vivo tissue Raman measurements at endoscopy.
abstract: We report an integrated Raman spectroscopy and trimodal (white-light reflectance, autofluorescence, and narrow-band) imaging techniques for real-time in vivo tissue Raman measurements at endoscopy. A special 1.8 mm endoscopic Raman probe with filtering modules is developed, permitting effective elimination of interference of fluorescence background and silica Raman in fibers while maximizing tissue Raman collections. We demonstrate that high-quality in vivo Raman spectra of upper gastrointestinal tract can be acquired within 1 s or subseconds under the guidance of wide-field endoscopic imaging modalities, greatly facilitating the adoption of Raman spectroscopy into clinical research and practice during routine endoscopic inspections.
authors.affiliation: Bioimaging Laboratory, Department of Bioengineering, Faculty of Engineering, National University of Singapore, and National University Hospital, Singapore. biehzw@nus.edu.sg
authors.forename: Zhiwei
authors.initials: Z
authors.lastname: Huang
authors.forename: Seng Khoon
authors.initials: SK
authors.lastname: Teh
authors.forename: Wei
authors.initials: W
authors.lastname: Zheng
authors.forename: Jianhua
authors.initials: J
authors.lastname: Mo
authors.forename: Kan
authors.initials: K
authors.lastname: Lin
authors.forename: Xiaozhuo
authors.initials: X
authors.lastname: Shao
authors.forename: Khek Yu
authors.initials: KY
authors.lastname: Ho
authors.forename: Ming
authors.initials: M
authors.lastname: Teh
authors.forename: Khay Guan
authors.initials: KG
authors.lastname: Yeoh
mesh_terms: D003199:Computer Systems; D019723:Endoscopes; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005336:Fiber Optic Technology / Q000295:instrumentation*; D006801:Humans; D007089:Image Enhancement / Q000379:methods*; D009061:Mouth Mucosa / Q000166:cytology*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013059:Spectrum Analysis, Raman / Q000295:instrumentation*; D018511:Systems Integration