Localization of reversion-induced LIM protein (RIL) in the rat central nervous system.
Reversion-induced LIM protein (RIL) is a member of the ALP (actinin-associated LIM protein) subfamily of the PDZ/LIM protein family. RIL serves as an adaptor protein and seems to regulate cytoskeletons. Immunoblotting suggested that RIL is concentrated in the astrocytes in the central nervous system. We then examined the expression and localization of RIL in the rat central nervous system and compared it with that of water channel aquaporin 4 (AQP4). RIL was concentrated in the cells of ependyma lining the ventricles in the brain and the central canal in the spinal cord. In most parts of the central nervous system, RIL was expressed in the astrocytes that expressed AQP4. Double-labeling studies showed that RIL was concentrated in the cytoplasm of astrocytes where glial fibrillary acidic protein was enriched as well as in the AQP4-enriched regions such as the endfeet or glia limitans. RIL was also present in some neurons such as Purkinje cells in the cerebellum and some neurons in the brain stem. Differential expression of RIL suggests that it may be involved in the regulation of the central nervous system.
Iida
Yuko
Y
Department of Anesthesiology and Medical Crisis Management, Nagoya City University Graduate School of Medical Sciences, Mizuho, Nagoya, Nagoya 467-8601, Japan.
Matsuzaki
Toshiyuki
T
Morishima
Tetsuro
T
Sasano
Hiroshi
H
Asai
Kiyofumi
K
Sobue
Kazuya
K
Takata
Kuniaki
K