Shank 2 expression coincides with neuronal differentiation in the developing retina.
The retinal activity for vision requires a precise synaptic connectivity. Shank proteins at postsynaptic sites of excitatory synapses play roles in signal transmission into the postsynaptic neuron. However, the correlation of Shank 2 expression with neuronal differentiation in the developing retina remains to be elucidated regardless of previous evidences of Shank 2 expression in retina. Herein, we demonstrated that with progression of development, Shank 2 is initially detected in the inner plexiform layer at P2, and then intensively detected in inner plexiform layer, outer plexiform layer, and ganglion cell layer at P14, which was closely colocalized to the neurofilament expression. Shank 2 was, however, not colocalized with glial fibrillary acidic protein. Shank 2 expression was increased in the differentiated retinoblastoma cells, which was mediated by ERK 1/2 activation. Moreover, Shank 2 expression was colocalized with neurofilament at the dendritic region of cells. In conclusion, our data suggests that Shank 2 is expressed in the neurons of the developing retina and could play a critical role in the neuronal differentiation of the developing retina.
Kim
Jeong Hun
JH
Department of Ophthalmology, Seoul National University College of Medicine, Seoul National University Hospital, Seoul 110-744, Korea.
Kim
Jin Hyoung
JH
Yang
Esther
E
Park
Jae Hwan
JH
Yu
Young Suk
YS
Kim
Kyu Won
KW
D048868:Adaptor Proteins, Signal Transducing / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D001253:Astrocytes / Q000166:cytology / Q000378:metabolism; D002454:Cell Differentiation / Q000502:physiology*; D004789:Enzyme Activation; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D051379:Mice; D008810:Mice, Inbred C57BL; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism*; D016900:Neurofilament Proteins / Q000378:metabolism; D009474:Neurons / Q000166:cytology / Q000502:physiology*; D012160:Retina / Q000166:cytology / Q000254:growth & development / Q000502:physiology; D012175:Retinoblastoma / Q000378:metabolism / Q000473:pathology
D048868:Adaptor Proteins, Signal Transducing; D009419:Nerve Tissue Proteins; D016900:Neurofilament Proteins; C488222:Shank2 protein, mouse; D048049:Extracellular Signal-Regulated MAP Kinases