title: [Establishment of fibroblast cell line and its biological characteristics in Matou goat].
abstract: Taking Matou goat ear margin as the study material, we succeeded in established a fibroblast cell line by the method of explant culture directly. Observations on morphology, dynamic growth, determination of viability, analysis of karyotype, test of microorganism and other characteristics were detected. Results showed: Population Doubling Time (PDT) of cells was approximately 36 h; Cell viability was 96.7% after thawing; The status of cell After passage was constant; Analysis of chromosomal karyotyps indicated that diploid (2n=60) account for 98% in the cell line. Every index in the cell line met all the standard quality controls of ATCC in USA. The established of Matou goat ear fibroblast cell line has not only important genetic resources preserved at the cell level, but also valuable material for genome, postgenome and somatic cell nuclear transfer research.
authors.affiliation: College of Animal Science & Technology, Gansu Agricultural University, Lanzhou 730050, China.
authors.forename: Tianda
authors.initials: T
authors.lastname: Li
authors.forename: Chousheng
authors.initials: C
authors.lastname: Liu
authors.forename: Zhigang
authors.initials: Z
authors.lastname: Wang
authors.forename: Liping
authors.initials: L
authors.lastname: Zhang
authors.forename: Xiuzhu
authors.initials: X
authors.lastname: Sun
authors.forename: Junjin
authors.initials: J
authors.lastname: Zhao
authors.forename: Fei
authors.initials: F
authors.lastname: Meng
authors.forename: Guihe
authors.initials: G
authors.lastname: Luo
authors.forename: Jinqing
authors.initials: J
authors.lastname: Zhu
mesh_terms: D000818:Animals; D002460:Cell Line; D002465:Cell Movement; D002470:Cell Survival; D002681:China; D004171:Diploidy; D004431:Ear, External / Q000166:cytology; D005347:Fibroblasts / Q000166:cytology*; D006041:Goats; D007621:Karyotyping