Comparing coherent and spontaneous Raman scattering under biological imaging conditions.
We compare imaging based on coherent and spontaneous Raman scattering (SpRS) under conditions relevant for biological imaging. Using a broadband laser source, we perform spectral domain imaging of polystyrene beads using coherent Stokes Raman scattering and SpRS and find comparable signal levels. Short interaction lengths, low molecule number, and low incident power all reduce the advantages available with coherent Raman methods. We present calculations to support our measurements.
Cui
Meng
M
Department of Physics and Biophysics, University of Michigan, Ann Arbor, MI 48109, USA.
Bachler
Brandon R
BR
Ogilvie
Jennifer P
JP
D003198:Computer Simulation; D007090:Image Interpretation, Computer-Assisted / Q000379:methods*; D008954:Models, Biological; D015336:Molecular Probe Techniques / Q000295:instrumentation; D019047:Phantoms, Imaging; D013059:Spectrum Analysis, Raman / Q000295:instrumentation / Q000379:methods*; D041623:Tomography, Optical Coherence / Q000295:instrumentation / Q000379:methods*