Substantially reduced expression of PIAS1 is associated with colon cancer development.
OBJECTIVE
Protein inhibitors of activated STATs (PIAS) regulate the interferon-gamma (IFN-gamma) signaling pathway, which has numerous effects on tumor development and tumor cell biology. PIAS's also regulate STAT family members not directly involved in IFN-gamma signaling. This project was designed to assess PIAS1 expression in colon cancer.


METHODS
To determine whether PIAS1, one of the PIAS family members, or IFN-gamma signaling pathway components could be used to stratify colon tumors, we stained tissue microarrays for PIAS1, interferon regulatory factor-1 (IRF-1) and STAT1alpha.


RESULTS
PIAS1 staining of the colon cancer tissue microarrays indicated a strong correlation of normal colon cells, and adenomas, with high expression of both PIAS1 and IRF-1.


CONCLUSIONS
The PIAS1 results in particular may represent a basis for new approaches for efficiently distinguishing adenomas from colon cancer.
Coppola
Domenico
D
Department of Anatomic Pathology, H Lee Moffitt Cancer Center and Research Institute, Tampa, USA. Domenico.Coppola@moffitt.org
Parikh
Vevek
V
Boulware
David
D
Blanck
George
G
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D003110:Colonic Neoplasms / Q000175:diagnosis / Q000378:metabolism* / Q000473:pathology*; D018450:Disease Progression; D015536:Down-Regulation; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D050856:Interferon Regulatory Factor-1 / Q000096:biosynthesis / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D050828:Protein Inhibitors of Activated STAT / Q000096:biosynthesis* / Q000172:deficiency* / Q000378:metabolism; D025841:Small Ubiquitin-Related Modifier Proteins / Q000096:biosynthesis* / Q000172:deficiency* / Q000378:metabolism; D046888:Tissue Array Analysis
D050856:Interferon Regulatory Factor-1; C106529:PIAS1 protein, human; D050828:Protein Inhibitors of Activated STAT; D025841:Small Ubiquitin-Related Modifier Proteins