Self-assembled nanogel of hydrophobized dendritic dextrin for protein delivery.
Highly branched cyclic dextrin derivatives (CH-CDex) that are partly substituted with cholesterol groups have been synthesized. The CH-CDex forms monodisperse and stable nanogels with a hydrodynamic radii of approximately 10 nm by the self-assembly of 4-6 CH-CDex macromolecules in water. The CH-CDex nanogels spontaneously trap 10-16 molecules of fluorescein isothiocyanate-labeled insulin (FITC-Ins). The complex shows high colloidal stability: no dissociation of trapped insulin is observed after at least 1 month in phosphate buffer (0.1 M, pH 8.0). In the presence of bovine serum albumin (BSA, 50 mg . mL(-1)), which is a model blood system, the FITC-Ins trapped in the nanogels is continuously released ( approximately 20% at 12 h) without burst release. The high-density nanogel structure derived from the highly branched CDex significantly affects the stability of the nanogel-protein complex.
Ozawa
Yayoi
Y
Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, 2-3-10, Kanda-surugadai, Chiyoda-ku, Tokyo 101-0062, Japan.
Sawada
Shin-Ichi
S
Morimoto
Nobuyuki
N
Akiyoshi
Kazunari
K
D002850:Chromatography, Gel; D002942:Circular Dichroism; D003692:Delayed-Action Preparations; D003912:Dextrins / Q000138:chemical synthesis* / Q000737:chemistry; D016503:Drug Delivery Systems / Q000379:methods*; D020100:Hydrogels / Q000138:chemical synthesis* / Q000737:chemistry; D007328:Insulin / Q000737:chemistry; D015394:Molecular Structure; D049329:Nanostructures / Q000737:chemistry*; D011506:Proteins / Q000008:administration & dosage*; D012710:Serum Albumin, Bovine
D003692:Delayed-Action Preparations; D003912:Dextrins; D020100:Hydrogels; D007328:Insulin; D011506:Proteins; D012710:Serum Albumin, Bovine