title: Periodate-resistant carbohydrate epitopes recognized by IgG and IgE antibodies from some of the immunized mice and patients with allergy.
abstract: The beta(1,2)-xylose- and/or alpha(1,3)-fucose-containing cross-reactive carbohydrate determinants (CCDs) are present in various plant and insect N-glycans, and have been attracted as potential antigens in IgE-mediated allergies and immunologically undesired post-translational products on some recombinant therapeutic proteins. By using ELISA and immunoblotting, CCDs-specific IgG and IgE antibodies from some, but not all, of mice and humans were found to fully retain their binding activity after a typical periodate-treatment to CCDs, which did cause the CCDs' antigenic activity to those from the other mice and rabbits to disappear almost completely. Furthermore, the mouse IgE recognizing the periodate-resistant CCDs induced the CCDs/IgE-dependent degranulation of rat basophilic RBL-2H3 cells. These findings indicate that in some cases CCDs include those dependent of the core trisaccharide more strongly than the terminal xylose and fucose, which might have been screened out in the CCDs analyses based on the loss of antibody-binding by the periodate-treatment.
authors.affiliation: Department of Applied Molecular Biosciences, Graduate School of Bio-agricultural Sciences, Nagoya University, Chikusa-ku, Nagoya 464-8601, Japan.
authors.forename: Shingo
authors.initials: S
authors.lastname: Hino
authors.forename: Takeshi
authors.initials: T
authors.lastname: Matsubara
authors.forename: Atsuo
authors.initials: A
authors.lastname: Urisu
authors.forename: Naohito
authors.initials: N
authors.lastname: Aoki
authors.forename: Chihiro
authors.initials: C
authors.lastname: Sato
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Okajima
authors.forename: Daita
authors.initials: D
authors.lastname: Nadano
authors.forename: Tsukasa
authors.initials: T
authors.lastname: Matsuda
mesh_terms: D000818:Animals; D000906:Antibodies / Q000276:immunology*; D000937:Antigen-Antibody Reactions; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay; D005643:Fucose / Q000276:immunology; D006735:Horseradish Peroxidase / Q000276:immunology; D006801:Humans; D006967:Hypersensitivity / Q000276:immunology; D015151:Immunoblotting; D016056:Immunodominant Epitopes / Q000737:chemistry / Q000276:immunology*; D007073:Immunoglobulin E / Q000276:immunology*; D007074:Immunoglobulin G / Q000276:immunology*; D051379:Mice; D010504:Periodic Acid / Q000737:chemistry; D011134:Polysaccharides / Q000276:immunology*; D011817:Rabbits; D014994:Xylose / Q000276:immunology
chemical_list: D000906:Antibodies; D016056:Immunodominant Epitopes; D007074:Immunoglobulin G; D011134:Polysaccharides; D010504:Periodic Acid; D005643:Fucose; D007073:Immunoglobulin E; D014994:Xylose; C009288:metaperiodate; D006735:Horseradish Peroxidase