[Determination of ferulic acid in Fuan oral liquid by high-performance capillary electrophoresis].
OBJECTIVE
To establish a quality control method of Fuan oral liquid.


METHODS
High-performance capillary electrophoresis (HPCE) was used to determine the content of feruild acid in Herba taraxaci, and thin-layer chromatography (TLC) was performed to identify Herba taraxaci and Bupleurum chinense. The condition of HPCE was optimized with a fuse silica capillary tube (70 microm x 60 cm) and 20 mmol/L sodium tetraborate buffer (pH9.18) at a constant voltage of 12 kV and temperature at 25 degrees celsius;, with the detection wavelength at 313 nm.


RESULTS
Clear spots were displayed on TLC. The calibration curve was linear in the range of 8-40 microg/ml for ferulic acid (Y=360.5-207.4, r=0.9997, n=5). The average recovery rate exceeded 95% with a RSD<3% (n=3).


CONCLUSIONS
This method is simple and specific with a good reproducibility for quality control of Fuan oral liquid.
Li
Yi-Lei
YL
Department of Pharmacy, Nanfang Hospital, Southern Medical University, Guangzhou, China. lei@fimmu.com
Zheng
Ping
P
Yan
Yuan
Y
Yang
Fang
F
D003373:Coumaric Acids / Q000032:analysis*; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D019075:Electrophoresis, Capillary / Q000379:methods*; D011786:Quality Control
D003373:Coumaric Acids; D004365:Drugs, Chinese Herbal; C004999:ferulic acid