A gene encoding SMALL ACIDIC PROTEIN 2 potentially mediates the response to synthetic auxin, 2,4-dichlorophenoxyacetic acid, in Arabidopsis thaliana.
The SMALL ACIDIC PROTEIN 2 (SMAP2) gene is a paralogue of the SMAP1 gene that mediates the response to the synthetic auxin 2,4-dichlorophenoxyacetic acid (2,4-D) in the root of Arabidopsis thaliana. Their encoded proteins, SMAP1 and SMAP2, are similar in calculated molecular weight and isoelectric point, and in having a highly conserved phenylalanine and aspartic acid-rich domain. RNA expression analysis showed that SMAP1 mRNA is present throughout the plant body while SMAP2 mRNA is restricted to siliques and anthers. Over-expression of the SMAP2 gene, as well as SMAP1, by 35S cauliflower mosaic virus promoter restored sensitivity to 2,4-D in the 2,4-D-resistant mutant, aar1, which is defective in SMAP1 function. The results suggest that SMAP2 has an ability to mediate the 2,4-D response and is expressed only in restricted tissues.
Nakasone
Akari
A
Radiation-Applied Biology Division, Japan Atomic Energy Agency, Takasaki 370-1292, Japan; Institute of Molecular and Cellular Biosciences, The University of Tokyo, Tokyo 113-0032, Japan. Electronic address: nakasone.akari@jaea.go.jp.
Kawai-Yamada
Maki
M
Institute of Molecular and Cellular Biosciences, The University of Tokyo, Tokyo 113-0032, Japan; Department of Environmental Science & Technology, Saitama University, Saitama 338-0825, Japan; Japan Science and Technology Agency, Core Research for Environmental Science and Technology, Kawaguchi 332-0012, Japan.
Kiyosue
Tomohiro
T
Divison of Genome Analysis and Genetic Research, Life Science Research Center, Institute of Research Promotion, Kagawa University, Kagawa 761-0795, Japan.
Narumi
Issay
I
Radiation-Applied Biology Division, Japan Atomic Energy Agency, Takasaki 370-1292, Japan.
Uchimiya
Hirofumi
H
Institute of Molecular and Cellular Biosciences, The University of Tokyo, Tokyo 113-0032, Japan; Iwate Biotechnology Research Center, Kitakami 024-0003, Japan.
Oono
Yutaka
Y
Radiation-Applied Biology Division, Japan Atomic Energy Agency, Takasaki 370-1292, Japan.
D015084:2,4-Dichlorophenoxyacetic Acid / Q000494:pharmacology*; D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000187:drug effects* / Q000235:genetics*; D029681:Arabidopsis Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D032461:Chromosomes, Plant / Q000235:genetics; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D017343:Genes, Plant; D007210:Indoleacetic Acids / Q000494:pharmacology*; D008969:Molecular Sequence Data; D017386:Sequence Homology, Amino Acid
D029681:Arabidopsis Proteins; D007210:Indoleacetic Acids; C514677:SMAP1 protein, Arabidopsis; D015084:2,4-Dichlorophenoxyacetic Acid