title: Mouse androgenetic embryonic stem cells differentiated to multiple cell lineages in three embryonic germ layers in vitro.
abstract: The embryos of some rodents and primates can precede early development without the process of fertilization; however, they cease to develop after implantation because of restricted expressions of imprinting genes. Asexually developed embryos are classified into parthenote/gynogenote and androgenote by their genomic origins. Embryonic stem cells (ESCs) derived from asexual origins have also been reported. To date, ESCs derived from parthenogenetic embryos (PgESCs) have been established in some species, including humans, and the possibility to be alternative sources for autologous cell transplantation in regenerative medicine has been proposed. However, some developmental characteristics, which might be important for therapeutic applications, such as multiple differentiation capacity and transplantability of the ESCs of androgenetic origin (AgESCs) are uncertain. Here, we induced differentiation of mouse AgESCs and observed derivation of neural cells, cardiomyocytes and hepatocytes in vitro. Following differentiated embryoid body (EB) transplantation in various mouse strains including the strain of origin, we found that the EBs could engraft in theoretically MHC-matched strains. Our results indicate that AgESCs possess at least two important characteristics, multiple differentiation properties in vitro and transplantability after differentiation, and suggest that they can also serve as a source of histocompatible tissues for transplantation.
authors.affiliation: Department of Obstetrics and Gynecology, Graduate School of Medicine, Mie University. teramura@med.kindai.ac.jp
authors.forename: Takeshi
authors.initials: T
authors.lastname: Teramura
authors.forename: Yuta
authors.initials: Y
authors.lastname: Onodera
authors.forename: Hideki
authors.initials: H
authors.lastname: Murakami
authors.forename: Syunsuke
authors.initials: S
authors.lastname: Ito
authors.forename: Toshihiro
authors.initials: T
authors.lastname: Mihara
authors.forename: Toshiyuki
authors.initials: T
authors.lastname: Takehara
authors.forename: Hiromi
authors.initials: H
authors.lastname: Kato
authors.forename: Tasuku
authors.initials: T
authors.lastname: Mitani
authors.forename: Masayuki
authors.initials: M
authors.lastname: Anzai
authors.forename: Kazuya
authors.initials: K
authors.lastname: Matsumoto
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Saeki
authors.forename: Kanji
authors.initials: K
authors.lastname: Fukuda
authors.forename: Norimasa
authors.initials: N
authors.lastname: Sagawa
authors.forename: Yoshihiko
authors.initials: Y
authors.lastname: Osoi
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000502:physiology*; D019070:Cell Lineage / Q000502:physiology*; D002478:Cells, Cultured; D004622:Embryo, Mammalian; D053595:Embryonic Stem Cells / Q000502:physiology*; D005260:Female; D005855:Germ Layers / Q000502:physiology*; D022781:Hepatocytes / Q000276:immunology / Q000502:physiology / Q000637:transplantation; D007108:Immune Tolerance / Q000502:physiology; D051379:Mice; D008810:Mice, Inbred C57BL; D032383:Myocytes, Cardiac / Q000276:immunology / Q000502:physiology / Q000637:transplantation; D009474:Neurons / Q000276:immunology / Q000502:physiology / Q000637:transplantation; D010312:Parthenogenesis / Q000502:physiology; D011247:Pregnancy