title: Development and validation of a reversed-phase HPLC method for the simultaneous analysis of simvastatin and tocotrienols in combined dosage forms.
abstract: A RP-HPLC method for the simultaneous analysis of tocotrienol isoforms (TRF) and simvastatin (SIM) in SIM-TRF nanoparticles (NPs) was developed. Analytes were monitored by UV detection at 238 and 295 nm for SIM and TRF, respectively, using a gradient methanol/water elution. Calibration curves for TRF and SIM were linear over concentration range of 20-80 microg/mL and 1-10 microg/mL with correlation coefficients 0.9990 and 0.9991, respectively. The recovery of TRF and SIM from the NPs was in the range from 97.35 to 102.19% and from 92.71 to 104.35%, respectively. This developed method was successfully employed in quantifying both drugs in NPs for future use in cancer therapy.
authors.affiliation: Department of Basic Pharmaceutical Sciences, College of Pharmacy, University of Louisiana at Monroe, Monroe, LA 71201, USA.
authors.forename: Hazem
authors.initials: H
authors.lastname: Ali
authors.forename: Sami
authors.initials: S
authors.lastname: Nazzal
mesh_terms: D000465:Algorithms; D000975:Antioxidants / Q000032:analysis*; D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D004304:Dosage Forms; D004338:Drug Combinations; D019161:Hydroxymethylglutaryl-CoA Reductase Inhibitors / Q000032:analysis*; D007536:Isomerism; D053758:Nanoparticles / Q000032:analysis; D011786:Quality Control; D012015:Reference Standards; D015203:Reproducibility of Results; D019821:Simvastatin / Q000032:analysis*; D012996:Solutions; D013056:Spectrophotometry, Ultraviolet; D024508:Tocotrienols / Q000032:analysis*
chemical_list: D000975:Antioxidants; D004304:Dosage Forms; D004338:Drug Combinations; D019161:Hydroxymethylglutaryl-CoA Reductase Inhibitors; D012996:Solutions; D024508:Tocotrienols; D019821:Simvastatin