Reduced expression of angiopoietin-1 in Hantaan virus-infected human umbilical vein endothelial cell increases their permeability.
Hemorrhagic fever with renal syndrome (HFRS) caused by Hantaan virus (HTNV) is characterized by vascular hemorrhage and acute renal failure. Angiopoietin-1 (Ang-1) is a glycoprotein that maintains vessel integrity and reduces endothelial permeability. We found that in HTNV-infected human umbilical vein endothelial cells (HUVECs) the levels of Ang-1 mRNA and protein were reduced on days 2 and 3 post-infection (p.i.), when endothelial permeability was increased. The HTNV-stimulated permeability was reduced by treatment of HUVECs with Ang-1. The plasma Ang-1 level was lower in HFRS patients than in healthy persons. Paired plasma samples of HFRS patients revealed markedly lower Ang-1 levels during the acute phase of HFRS as compared to the convalescent phase. These findings suggested that HTNV reduced the Ang-1 expression in endothelial cells that might play an important role in the increase of vascular permeability in HFRS.
Hwang
J Y
JY
Department of Microbiology, Yuengnam University, Daegu, Korea.
Park
J W
JW
Hong
S Y
SY
Park
H S
HS
D042683:Angiopoietin-1 / Q000037:antagonists & inhibitors* / Q000096:biosynthesis* / Q000097:blood; D002199:Capillary Permeability; D002478:Cells, Cultured; D042783:Endothelial Cells / Q000821:virology*; D020869:Gene Expression Profiling; D018063:Hantaan virus / Q000502:physiology*; D006801:Humans; D010949:Plasma / Q000737:chemistry; D014471:Umbilical Veins / Q000166:cytology
D042683:Angiopoietin-1