title: Differential cofactor requirements for histone eviction from two nucleosomes at the yeast PHO84 promoter are determined by intrinsic nucleosome stability.
abstract: We showed previously that the strong PHO5 promoter is less dependent on chromatin cofactors than the weaker coregulated PHO8 promoter. In this study we asked if chromatin remodeling at the even stronger PHO84 promoter was correspondingly less cofactor dependent. The repressed PHO84 promoter showed a short hypersensitive region that was flanked upstream and downstream by a positioned nucleosome and contained two transactivator Pho4 sites. Promoter induction generated an extensive hypersensitive and histone-depleted region, yielding two more Pho4 sites accessible. This remodeling was strictly Pho4 dependent, strongly dependent on the remodelers Snf2 and Ino80 and on the histone acetyltransferase Gcn5, and more weakly on the acetyltransferase Rtt109. Importantly, remodeling of each of the two positioned nucleosomes required Snf2 and Ino80 to different degrees. Only remodeling of the upstream nucleosome was strictly dependent on Snf2. Further, remodeling of the upstream nucleosome was more dependent on Ino80 than remodeling of the downstream nucleosome. Both nucleosomes differed in their intrinsic stabilities as predicted in silico and measured in vitro. The causal relationship between the different nucleosome stabilities and the different cofactor requirements was shown by introducing destabilizing mutations in vivo. Therefore, chromatin cofactor requirements were determined by intrinsic nucleosome stabilities rather than correlated to promoter strength.
authors.affiliation: Adolf-Butenandt-Institut, Universität München, Munich, Germany.
authors.forename: Christian J
authors.initials: CJ
authors.lastname: Wippo
authors.forename: Bojana Silic
authors.initials: BS
authors.lastname: Krstulovic
authors.forename: Franziska
authors.initials: F
authors.lastname: Ertel
authors.forename: Sanja
authors.initials: S
authors.lastname: Musladin
authors.forename: Dorothea
authors.initials: D
authors.lastname: Blaschke
authors.forename: Sabrina
authors.initials: S
authors.lastname: Stürzl
authors.forename: Guo-Cheng
authors.initials: GC
authors.lastname: Yuan
authors.forename: Wolfram
authors.initials: W
authors.lastname: Hörz
authors.forename: Philipp
authors.initials: P
authors.lastname: Korber
authors.forename: Slobodan
authors.initials: S
authors.lastname: Barbaric
mesh_terms: D001483:Base Sequence; D001665:Binding Sites; D042002:Chromatin Assembly and Disassembly; D015966:Gene Expression Regulation, Fungal; D006657:Histones / Q000378:metabolism*; D007700:Kinetics; D009690:Nucleic Acid Conformation; D009707:Nucleosomes / Q000737:chemistry* / Q000378:metabolism*; D011401:Promoter Regions, Genetic; D028081:Proton-Phosphate Symporters / Q000235:genetics*; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*; D017384:Sequence Deletion; D016385:TATA Box / Q000235:genetics; D014158:Transcription, Genetic
chemical_list: D006657:Histones; D009707:Nucleosomes; C105495:PHO84 protein, S cerevisiae; D028081:Proton-Phosphate Symporters; D029701:Saccharomyces cerevisiae Proteins