Refinement of canine pancreatitis model: inducing pancreatitis by using endoscopic retrograde cholangiopancreatography.
The causes and treatments of pancreatitis have been studied in diverse species, but the canine pancreatitis model has been used most often due to its similarities to the condition in humans. Although pancreatitis in dogs can be induced readily by numerous methods, managing these dogs can be difficult because they often develop severe abdominal pain, vomiting, inappetance, and lethargy. In an effort to study pancreatitis, we performed a pilot study to determine whether an endoscopic pancreatic procedure would be possible in a dog and whether, through various manipulations, a new method of inducing pancreatitis could be developed. The model uses endoscopic retrograde cholangiopancreatography (ERCP), a common procedure in human gastroenterology that has been associated with postprocedural pancreatitis. Although all 8 dogs used in developing the ERCP model had both biochemical and histologic changes consistent with pancreatitis, 7 of the 8 dogs remained free of classic clinical signs of the disease. This method is presented as a refinement of a canine model and presents an alternative method of inducing pancreatitis, with decreased risk of developing associated clinical signs.
Ruben
Dawn S
DS
Johns Hopkins University School of Medicine, Baltimore, Maryland, USA. druben1@jhmi.edu
Scorpio
Diana G
DG
Gabrielson
K L
KL
Simon
B W
BW
Buscaglia
Jonathan M
JM
D000208:Acute Disease; D000681:Amylases / Q000097:blood; D000818:Animals; D002760:Cholangiopancreatography, Endoscopic Retrograde / Q000009:adverse effects* / Q000379:methods*; D004195:Disease Models, Animal; D004285:Dogs; D005471:Fluoroscopy; D008049:Lipase / Q000097:blood; D008297:Male; D010179:Pancreas / Q000473:pathology*; D010195:Pancreatitis / Q000097:blood / Q000209:etiology* / Q000473:pathology*; D010865:Pilot Projects; D011183:Postoperative Complications
D008049:Lipase; D000681:Amylases