A general microchip surface modification approach using a spin-coated polymer resist film doped with hydroxypropyl cellulose.
We have developed a simple and effective method for surface modification of polymer microchips by entrapping hydroxypropyl cellulose (HPC) in a spin-coated thin film on the surface. Poly(methyl methacrylate-8.5-methacrylic acid), a widely available commercial resist formulation, was utilized as a matrix for dissolving HPC and providing adherence to native polymer surfaces. Various amounts of HPC (0.1-2.0%) dissolved in the copolymer and spun on polymer surfaces were evaluated. The modified surfaces were characterized by contact angle measurement, X-ray photoelectron spectroscopy and atomic force microscopy. The developed method was applied on both poly(methyl methacrylate) and cyclic olefin copolymer microchips. A fluorescently labeled myoglobin digest, binary protein mixture, and human serum sample were all separated in these surface-modified polymer microdevices. Our work exhibits an easy and reliable way to achieve favorable biomolecular separation performance in polymer microchips.
Sun
Xiuhua
X
Department of Chemistry and Biochemistry, Brigham Young University, Provo, Utah 84602-5700, USA.
Yang
Weichun
W
Geng
Yanli
Y
Woolley
Adam T
AT
D000327:Adsorption; D000818:Animals; D001639:Bicarbonates / Q000737:chemistry; D002021:Buffers; D002482:Cellulose / Q000031:analogs & derivatives* / Q000737:chemistry / Q000648:ultrastructure; D020099:Coated Materials, Biocompatible / Q000737:chemistry; D003188:Composite Resins; D003516:Cycloparaffins / Q000737:chemistry; D016650:Fluorescein-5-isothiocyanate / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry; D006736:Horses; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008689:Methacrylates / Q000737:chemistry; D018625:Microscopy, Atomic Force; D009211:Myoglobin / Q000378:metabolism; D011108:Polymers / Q000737:chemistry*; D019904:Polymethyl Methacrylate / Q000737:chemistry; D044967:Serum / Q000737:chemistry; D013499:Surface Properties; D013997:Time Factors
D001639:Bicarbonates; D002021:Buffers; D020099:Coated Materials, Biocompatible; D003188:Composite Resins; D003516:Cycloparaffins; D005456:Fluorescent Dyes; D008689:Methacrylates; D009211:Myoglobin; D011108:Polymers; C420128:Spectrum composite resin; C008384:methacrylic acid; D002482:Cellulose; D019904:Polymethyl Methacrylate; C008079:hydroxypropylcellulose; D016650:Fluorescein-5-isothiocyanate