Isolation and characterization of Rhodococcus sp. Y22 and its potential application to tobacco processing.
A novel nicotine-degrading bacterium, strain Y22, was isolated and identified as Rhodococcus sp. Y22 based on its 16S rDNA sequence and morphological and biochemical features. The isolate could utilize nicotine as the sole source of carbon and nitrogen. Nicotine (1.0g/L) was degraded by Rhodococcus sp. Y22 within 52h at 28 degrees C and pH 7.0. Preparation of resting cells from nicotine-induced cultures was found to rapidly and efficiently degrade nicotine from solutions as well as from tobacco leaves. Therefore, Rhodococcus sp. Y22 has the potential to degrade nicotine during tobacco leave processing.
Gong
Xiao-Wei
XW
Laboratory for Conservation and Utilization of Bio-resources, and Key Laboratory for Microbial Resources of the Ministry of Education, Yunnan University, Kunming, People's Republic of China.
Yang
Jin-Kui
JK
Duan
Yan-Qin
YQ
Dong
Jin-Yan
JY
Zhe
Wei
W
Wang
Le
L
Li
Qing-Hua
QH
Zhang
Ke-Qin
KQ
D015373:Bacterial Typing Techniques; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D006863:Hydrogen-Ion Concentration; D007218:Industrial Microbiology; D008969:Molecular Sequence Data; D009538:Nicotine / Q000378:metabolism*; D010802:Phylogeny; D018515:Plant Leaves / Q000378:metabolism; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012240:Rhodococcus / Q000145:classification* / Q000166:cytology / Q000302:isolation & purification / Q000378:metabolism*; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D013696:Temperature; D013997:Time Factors; D014026:Nicotiana / Q000378:metabolism
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D009538:Nicotine