[Effects of the micro-arc oxidized film on pure titanium surface on attachment and growth of BMSCs].
OBJECTIVE
To evaluate the biocompatibility of oxide film formed by micro-arc oxidation(MAO) on titanium surface by culturing bone marrow stromal cells(BMSCs) on its surface and observing the BMSCs's biological behavior of early adhesion, growth and alkaline phosphatase activity.


METHODS
According to the surface treatments, the samples were classified into 4 groups: MAO group, abrator group, abrator and NaOH-treated group, and untreated titanium group. Attachment, growth and alkaline phosphatase (ALP) activity of BMSCs cultured on the titanium surface of each group were assessed at different time. All data were statistically analyzed with one-way ANOVA using SPSS11.5 software package.


RESULTS
From a morphological point of view, cell adherence in the MAO group was the best. Cell count and ALP activity in the MAO group were highest(P<0.01).


CONCLUSIONS
MAO film on pure titanium surface may increase BMSCs adhesion, growth and ALP activity and titanium surface treated with MAO exhibits better biocompatibility.
Chen
Jian-zhi
JZ
Department of Stomatology, Zhejiang Traditional Medical University. Hangzhou 310005, China. chenj_z@163.com
Peng
Hong-yan
HY
Zhang
Xiu-li
XL
D002448:Cell Adhesion; D010084:Oxidation-Reduction; D014025:Titanium
D014025:Titanium