CXCR4 receptor positive spheroid forming cells are responsible for tumor invasion in vitro.
Stem cells have been found to be involved in breast cancer growth, but the specific contribution of cancer stem cells in tumor biology, including metastasis, is still uncertain. We found that murine breast cancer cell lines 4T1, 4TO7, 167Farn and 67NR contains cancer stem cells defined by CXCR4 expression and their capability of forming spheroids in suspension culture. Importantly, we showed that CXCR4 expression is essential for tumor invasiveness because both CXCR4 neutralizing antibody and shRNA knockdown of the CXCR4 receptor significantly reduced tumor cell invasion.
Krohn
Alexander
A
Department of Molecular Pathology, University of Texas, M.D. Anderson Cancer Center, Houston, TX 77030, USA.
Song
Yao-Hua
YH
Muehlberg
Fabian
F
Droll
Lilly
L
Beckmann
Christoph
C
Alt
Eckhard
E
D000818:Animals; D045744:Cell Line, Tumor; D002465:Cell Movement; D006801:Humans; D066298:In Vitro Techniques; D015674:Mammary Neoplasms, Animal / Q000378:metabolism; D051379:Mice; D008954:Models, Biological; D009361:Neoplasm Invasiveness; D009362:Neoplasm Metastasis; D014411:Neoplastic Stem Cells; D034741:RNA, Small Interfering / Q000378:metabolism; D019718:Receptors, CXCR4 / Q000096:biosynthesis* / Q000378:metabolism; D018874:Spheroids, Cellular / Q000378:metabolism*; D014407:Tumor Cells, Cultured
D034741:RNA, Small Interfering; D019718:Receptors, CXCR4