Analytical quantification of low-density lipoprotein using europium tetracycline indicator.
Low-density lipoprotein (LDL) is known as 'bad' cholesterol. If too much LDL circulates in the blood it can be retained in the walls of the arteries, causing atherosclerosis. In this paper we showed an alternative method to quantify LDL using the europium tetracycline (EuTc) indicator. The optical properties of the EuTc complex were investigated in aqueous solutions containing LDL. An enhancement was observed of the europium luminescence in the solutions with LDL compared those without the lipoprotein. A method to quantify the amount of LDL in a sample, based on EuTc enhanced luminescence, is proposed. The enhancement mechanism is also discussed.
De Oliveira Silva
Flávia Rodrigues
FR
Universidade Federal de São Paulo UNIFESP, Departamento de Medicina, Disciplina de Nefrologia, São Paulo, Brazil.
Monteiro
Andrea Moreira
AM
Neto
Antônio M Figueiredo
AM
Gidlund
Magnus
M
Junior
Nilson Dias Vieira
ND
Courrol
Lilia Coronato
LC
D006801:Humans; D007202:Indicators and Reagents / Q000032:analysis* / Q000737:chemistry*; D008077:Lipoproteins, LDL / Q000097:blood*; D049449:Luminescence; D009942:Organometallic Compounds / Q000032:analysis / Q000737:chemistry*; D012016:Reference Values; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D013754:Tetracyclines / Q000032:analysis / Q000737:chemistry*
D007202:Indicators and Reagents; D008077:Lipoproteins, LDL; D009942:Organometallic Compounds; D013754:Tetracyclines; C488317:europium(III) tetracycline