title: A concanavalin A-like lectin domain in the CHS1/LYST protein, shared by members of the BEACH family.
abstract: CHS1/LYST, the causative protein of the Chediak-Higashi syndrome (CHS), belongs to the BEACH (named after BEige And Chediak-Higashi) family, which includes various large proteins sharing the same C-terminal domain architecture [a PH (Pleckstrin homology)-BEACH domain followed by WD repeats). Members of the BEACH family are generally defined as vesicle-trafficking regulatory proteins, but their functions remain to be determined at the molecular level. Here, using a panel of sensitive methods of sequence analysis, we show that the N-terminal regions of BEACH proteins contain an as yet not described domain, which shares striking similarities with clostridial neurotoxins and defines a novel family within the concanavalin A (ConA)-like lectin superfamily. These results suggest that the BEACH ConA-like lectin domain could be involved in oligosaccharide binding associated with protein traffic and sorting along the secretory pathway, especially in relation with components of the vesicle fusion machinery.
authors.affiliation: INSERM, U768, Paris, F-75015, France.
authors.forename: Agathe
authors.initials: A
authors.lastname: Burgess
authors.forename: Jean-Paul
authors.initials: JP
authors.lastname: Mornon
authors.forename: Geneviève
authors.initials: G
authors.lastname: de Saint-Basile
authors.forename: Isabelle
authors.initials: I
authors.lastname: Callebaut
mesh_terms: D000595:Amino Acid Sequence; D001665:Binding Sites; D019295:Computational Biology / Q000379:methods*; D003208:Concanavalin A / Q000737:chemistry*; D030562:Databases, Protein; D008969:Molecular Sequence Data; D017510:Protein Folding; D017434:Protein Structure, Tertiary; D016415:Sequence Alignment; D033921:Vesicular Transport Proteins / Q000737:chemistry*
chemical_list: C102161:LYST protein, human; D033921:Vesicular Transport Proteins; D003208:Concanavalin A