title: [Sperm acrosin activity helps predict IVF-ET outcome].
abstract: OBJECTIVE
  To investigate the effect of sperm acrosin activity on the IVF-ET outcome.
  
  
  METHODS
  We analyzed sperm parameters, morphology and acrosin activity for 909 infertile husbands by computer-assisted self-assessment (CASA), modified Papanicolaou staining and N-alpha-benzoyl-DL-arginine-p-nitroanilide (BAPNA), respectively, and detected the rates of fertilization, cleavage, quality embryos, embryo cryopreservation, implantation, clinical pregnancy and abortion. The wives were identified as normal or with mere oviduct problems.
  
  
  RESULTS
  The rate of normal sperm morphology and sperm motility, vitality, rapid progressive velocity and concentration were significantly lower in the abnormal acrosin activity group than in the normal one (P < 0.01). Significant positive correlations were observed between acrosin activity and the above-mentioned semen parameters (P < 0.01). There were no significant differences in the number of retrieved eggs, the rates of cleavage, quality embryos, embryo cryopreservation, non-embryo transfer cycles and miscarriages, and the number of transferred embryos between the two groups (P > 0.05). The fertilization rate, the percentage of transfer cycles with only 1 embryo and the rate of implantation and clinical pregnancy were notably higher in the normal acrosin activity group than in the abnormal one (P < 0.01).
  
  
  CONCLUSIONS
  Sperm acrosin activity is closely related with semen parameters, and it helps to predict the sperm fertilizing capacity and IVF-ET outcome.
authors.affiliation: Guangzhou Key Laboratory of Reproduction and Genetics, The Third Affiliated Hospital of Guangzhou Medical College, Guangzhou, Guangdong 510150, China.
authors.forename: Xin-Jie
authors.initials: XJ
authors.lastname: Chen
authors.forename: Xiao-Lin
authors.initials: XL
authors.lastname: Long
authors.forename: Xiao-Fang
authors.initials: XF
authors.lastname: Sun
authors.forename: Wei-Liang
authors.initials: WL
authors.lastname: Zhang
authors.forename: Xue-Shi
authors.initials: XS
authors.lastname: Wu
authors.forename: Yu-Mei
authors.initials: YM
authors.lastname: Luo
authors.forename: Hong-Zi
authors.initials: HZ
authors.lastname: Du
authors.forename: Bao-Ping
authors.initials: BP
authors.lastname: Liao
authors.forename: Hua
authors.initials: H
authors.lastname: Zhou
mesh_terms: D000176:Acrosin / Q000378:metabolism*; D000328:Adult; D004624:Embryo Transfer; D005260:Female; D005307:Fertilization in Vitro; D006801:Humans; D007248:Infertility, Male; D008297:Male; D011247:Pregnancy; D018873:Pregnancy Rate; D055101:Semen Analysis; D013094:Spermatozoa / Q000201:enzymology*
chemical_list: D000176:Acrosin