Immunohistochemical detection of the Raf kinase inhibitor protein in nonneoplastic gastric tissue and gastric cancer tissue.
Expression of the Raf kinase inhibitor protein (RKIP), a metastasis suppressor, is high in normal tissues, low in primary cancers, and lowest or absent in metastatic cancers. Here, we studied the expression of RKIP in nonneoplastic gastric tissue and gastric cancer tissue by immunohistochemistry (IHC) and evaluated its role in the genesis and metastasis of gastric cancer. RKIP immunoreactivity was evaluated in 40 samples of nonneoplastic gastric tissues and 75 samples of gastric cancer tissues. Among the 40 samples of nonneoplastic gastric tissue, 35 (87.5%) were positive for RKIP expression and 5 (12.5%) were negative; in the 75 samples of primary gastric cancer tissue, 39 (52%) were positive for RKIP expression and 36 (48%) were negative. Among 26 samples of metastatic lymph node tissues, 5 (19.2%) were positive for RKIP expression and 21 (80.8%) were negative. RKIP expression level was highest in nonneoplastic gastric tissue, low in primary gastric cancer tissue, and lowest or undetectable in metastatic gastric cancer tissue. Our data suggest that RKIP may play a role in the genesis and metastasis of gastric cancer.
Wang
Jin
J
Department of Surgery, LuZhou Medical College, LuZhou, Sichuan, People's Republic of China.
Yang
Yong-Hong
YH
Wang
An-Qun
AQ
Yao
Bing
B
Xie
Gang
G
Feng
Gang
G
Zhang
Yu
Y
Cheng
Zong-Shu
ZS
Hui
Li
L
Dai
Tang-Zhi
TZ
Du
Xiao-Bo
XB
Wang
Dong
D
D014408:Biomarkers, Tumor / Q000096:biosynthesis*; D005753:Gastric Mucosa / Q000201:enzymology / Q000378:metabolism*; D005759:Gastroenteritis / Q000175:diagnosis / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D049488:Phosphatidylethanolamine Binding Protein / Q000032:analysis / Q000096:biosynthesis*; D013274:Stomach Neoplasms / Q000175:diagnosis / Q000201:enzymology / Q000378:metabolism*
D014408:Biomarkers, Tumor; D049488:Phosphatidylethanolamine Binding Protein