title: Cloning and characterization of the Rubisco activase gene from Ipomoea batatas (L.) Lam.
abstract: A full-length cDNA of Rubisco activase (IBrcaI) was cloned from sweet potato (Ipomoea batatas (L.) Lam) using Rapid-Amplification of cDNA Ends (RACE). IBrcaI contains a 1,347 bp open reading frame encoding a protein of 439 amino acids. The sequence alignment of multiple Rubisco activase genes from sweet potato and other plants showed high homology at two previously described ATP-binding sites. Western blot analysis indicated that there are two Rubisco activase proteins in sweet potato. Expression of IBrcaI was only detected in leaves. In the 14 h light and 10 h dark photoperiods, maximal and minimal IBrcaI mRNA expression levels were detected at 8:00 in the morning and at midnight, respectively.
authors.affiliation: Sichuan Key Laboratory of Molecular Biology and Biotechnology, College of Life Science, Sichuan University, 610064, Chengdu, People's Republic of China. xkkglj37@yahoo.com.cn
authors.forename: Ke
authors.initials: K
authors.lastname: Xu
authors.forename: Bowen
authors.initials: B
authors.lastname: He
authors.forename: Shuang
authors.initials: S
authors.lastname: Zhou
authors.forename: Yi
authors.initials: Y
authors.lastname: Li
authors.forename: Yizheng
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000302:isolation & purification; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D027723:Ipomoea batatas / Q000235:genetics*; D008969:Molecular Sequence Data; D010802:Phylogeny; D010940:Plant Proteins / Q000235:genetics* / Q000302:isolation & purification; D017386:Sequence Homology, Amino Acid
chemical_list: D018076:DNA, Complementary; D010940:Plant Proteins; C054765:rca protein, plant