title: Specific induction of a 72-kDa heat shock protein protects esophageal mucosa from reflux esophagitis.
abstract: OBJECTIVE
  The aim of this study is to investigate the expression and cytoprotective function of a 72-kDa heat shock protein (HSP72) using a reflux esophagitis model in rats.
  
  
  METHODS
  Expression of HSP60, HSP72, and HSP90 in rat esophageal mucosa was evaluated by Western blot analysis before and after hyperthermia (42.5 degrees C, 20 min). Rats received the operation to produce reflux esophagitis with or without pretreatment with hyperthermia to induce HSPs. The esophageal mucosal damage was evaluated 12 h after the operation.
  
  
  RESULTS
  Expression of HSP72 was significantly increased by hyperthermia in rat esophageal mucosa. Reflux esophagitis was dramatically prevented when HSP72 was preinduced by hyperthermia. Furthermore, activation of TNF-alpha and IL-1beta in esophageal mucosa was also suppressed.
  
  
  CONCLUSIONS
  These results suggested that hyperthermia protects the esophageal mucosa in reflux esophagitis model by inducing HSP72 and suppressing proinflammatory cytokine activation. These findings might suggest that HSP-inducing therapy could be a novel and unique therapy for reflux esophagitis.
authors.affiliation: Department of Gastroenterology, Juntendo University School of Medicine, 2-1-1 Hongo, Bunkyo-Ku, Tokyo, Japan.
authors.forename: Yuko
authors.initials: Y
authors.lastname: Izumi
authors.forename: Michiro
authors.initials: M
authors.lastname: Otaka
authors.forename: Taiji
authors.initials: T
authors.lastname: Takahashi
authors.forename: Makiko
authors.initials: M
authors.lastname: Takada
authors.forename: Yuji
authors.initials: Y
authors.lastname: Shimada
authors.forename: Daisuke
authors.initials: D
authors.lastname: Asaoka
authors.forename: Akihito
authors.initials: A
authors.lastname: Nagahara
authors.forename: Hideaki
authors.initials: H
authors.lastname: Itoh
authors.forename: Sumio
authors.initials: S
authors.lastname: Watanabe
mesh_terms: D000818:Animals; D015153:Blotting, Western; D004195:Disease Models, Animal; D004942:Esophagitis, Peptic / Q000378:metabolism* / Q000517:prevention & control*; D050884:HSP72 Heat-Shock Proteins / Q000096:biosynthesis*; D006979:Hyperthermia, Induced; D008297:Male; D009092:Mucous Membrane / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D050884:HSP72 Heat-Shock Proteins