[Influence of electromagnetic radiation on raf kinase inhibitor protein and its related proteins of hippocampus].
OBJECTIVE
To study the development of changes for Raf kinase inhibitor protein (RKIP) and its mRNA in rats hippocampus after electromagnetic radiation.


METHODS
Rats were exposed to X-band high power microwave (X-HPM), S-band high power microwave (S-HPM) and electromagnetic pulse (EMP) radiation source respectively. The animal model of electromagnetic radiation was established. Western blot was used to detect the expression of RKIP, and RT-PCR was applied to detect the expression of RKIP mRNA. The interaction of RKIP and Raf-1 was measured with co-immunoprecipitation method, and the expression of cerebral choline acetyltransferase (CHAT) was measured by immunohistochemistry.


RESULTS
The expression of RKIP significantly down-regulated at 6 h after radiation, and recovered at 1 d in group EMP, but the down-regulation continued during 1 approximately 7 d after radiation in the two microwave groups. The expression of RKIP mRNA changed wavily during 6 h approximately 7 d after radiation, which showed down-regulation at 6 h, and up-regulation at 3 d. The interaction of RKIP and Raf-1 decreased during 6 h approximately 7 d after radiation, most significantly at 7 d, and the two microwave groups were more significant. The expression of CHAT decreased continuously during 6 h approximately 7 d after radiation, and generally recovered on 14 d.


CONCLUSIONS
The down-regulation of RKIP and its related proteins of hippocampus is induced by electromagnetic radiation.
Zuo
Hong-yan
HY
Institute of Radiation Medicine, Academy of Military Medical Science, Beijing 100850, China.
Wang
De-wen
DW
Peng
Rui-yun
RY
Wang
Shui-ming
SM
Gao
Ya-bing
YB
Xu
Xin-ping
XP
Ma
Jun-jie
JJ
D000818:Animals; D060733:Electromagnetic Radiation; D006624:Hippocampus / Q000378:metabolism / Q000528:radiation effects; D020930:MAP Kinase Kinase Kinases / Q000378:metabolism; D008297:Male; D049488:Phosphatidylethanolamine Binding Protein / Q000235:genetics / Q000378:metabolism*; D019908:Proto-Oncogene Proteins c-raf; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D017208:Rats, Wistar
C550693:PEBP1 protein, rat; D049488:Phosphatidylethanolamine Binding Protein; D012333:RNA, Messenger; D019908:Proto-Oncogene Proteins c-raf; C546782:Raf1 protein, rat; D020930:MAP Kinase Kinase Kinases