Molecular recognition by contact angle: proof of concept with DNA hybridization.
A molecular recognition reaction supported by a solid-phase assay drives a specific change in the solid-solution interfacial tension. This prompts contact angle (CA) analysis, being a straightforward route to evaluate this property, to play the unedited role of label-free probe of the reaction. The concept is proven by the successful recognition of DNA hybridization and is further supported by the agreement between the results and the underpinning thermodynamics.
Bergese
Paolo
P
Chemistry for Technologies Laboratory and INSTM, University of Brescia, Via Branze 38, 25123 Brescia, Italy. paolo.bergese@ing.unibs.it
Oliviero
Giulio
G
Colombo
Italo
I
Depero
Laura E
LE
D055592:Biophysical Phenomena; D001703:Biophysics; D015374:Biosensing Techniques; D002021:Buffers; D004247:DNA / Q000737:chemistry*; D015342:DNA Probes / Q000737:chemistry*; D004277:DNA, Single-Stranded / Q000737:chemistry; D007700:Kinetics; D008024:Ligands; D009693:Nucleic Acid Hybridization; D020349:Surface Plasmon Resonance; D013499:Surface Properties; D013816:Thermodynamics; D013997:Time Factors
D002021:Buffers; D015342:DNA Probes; D004277:DNA, Single-Stranded; D008024:Ligands; D004247:DNA