Isolation of xanthyletin, an inhibitor of ants' symbiotic fungus, by high-speed counter-current chromatography.
Xanthyletin, an inhibitor of symbiotic fungus (Leucoagaricus gongylophorus) of leaf-cutting ant (Atta sexdens rubropilosa), as well as suberosin, seselin and xanthoxyletin were isolated from Citrus sinensis grafted on Citrus limonia. A two-phase solvent system composed of hexane/ethanol/acetonitrile/water (10:8:1:1, v/v) was used for the high-speed counter-current chromatographic isolation of xanthyletin with high yield and over 99% purity as determined by liquid and gas chromatography with mass spectrometry detection. Identifications were performed by UV spectra, IR spectra, (1)H NMR and (13)C NMR.
Cazal
Cristiane de Melo
Cde M
Universidade Federal de São Carlos, Departamento de Química, SP, Brazil.
Domingues
Vanessa de Cássia
Vde C
Batalhão
Jaqueline Raquel
JR
Bueno
Odair Corrêa
OC
Filho
Edson Rodrigues
ER
da Silva
Maria Fátima G Fernandes
MF
Vieira
Paulo Cezar
PC
Fernandes
João Batista
JB
D000818:Animals; D000935:Antifungal Agents / Q000737:chemistry / Q000302:isolation & purification*; D001000:Ants / Q000382:microbiology; D001487:Basidiomycota; D002957:Citrus / Q000737:chemistry; D032084:Citrus sinensis / Q000737:chemistry; D003374:Coumarins / Q000737:chemistry / Q000302:isolation & purification*; D003377:Countercurrent Distribution / Q000379:methods*; D010936:Plant Extracts / Q000737:chemistry*; D018517:Plant Roots / Q000737:chemistry; D012997:Solvents / Q000737:chemistry; D013559:Symbiosis
D000935:Antifungal Agents; D003374:Coumarins; D010936:Plant Extracts; D012997:Solvents; C020814:xanthyletine