Congenital candidiasis: confirmation of mother-neonate transmission using molecular analysis techniques.
We describe a case of congenital acquired candidiasis in a preterm female delivered through Caesarean section due to the premature rupture of the amniotic membrane. The neonate presented with suspected chorioamnionitis and erythematous desquamative skin. Candida albicans was isolated from the placenta, mouth, groin, and periumbilical lesions. The infant developed candidemia due to Candida albicans and the same yeast was also isolated from a catheter. Culture inoculated with swabs from the mouth and vagina of the mother yielded C. albicans and C. krusei. All C. albicans isolates from the mother and the neonate were visually indistinguishable by molecular typing techniques which included chromosomal karyotyping and restriction endonuclease analysis followed by pulsed-field gel electrophoresis. These findings allowed the clinical condition to be confirmed as congenital acquisition of candidiasis in this case.
Tiraboschi
Iris Carla Niveyro
IC
Infectious Diseases Division, Hospital de Clínicas José de San Martín University of Buenos Aires, Buenos Aires, Argentina. micologia@hospitaldeclinicas.uba.ar
Niveyro
Carla
C
Mandarano
Ana Maria
AM
Messer
Shawn A
SA
Bogdanowicz
Elizabeth
E
Kurlat
Isabel
I
Lasala
María Beatriz
MB
D002176:Candida albicans / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D002177:Candidiasis / Q000175:diagnosis* / Q000635:transmission*; D002404:Catheterization; D016172:DNA Fingerprinting; D004783:Environmental Microbiology; D005260:Female; D016469:Fungemia / Q000382:microbiology; D005838:Genotype; D006119:Groin / Q000382:microbiology; D006801:Humans; D007231:Infant, Newborn; D018445:Infectious Disease Transmission, Vertical; D007621:Karyotyping; D009035:Mothers; D009055:Mouth / Q000382:microbiology; D016533:Mycological Typing Techniques; D010920:Placenta / Q000382:microbiology; D012150:Polymorphism, Restriction Fragment Length; D011247:Pregnancy; D011251:Pregnancy Complications, Infectious / Q000382:microbiology*; D047928:Premature Birth; D014472:Umbilicus / Q000382:microbiology