title: Expression of nucleosomal protein HMGN1 in the cycling mouse hair follicle.
abstract: Here we examine the expression pattern of HMGN1, a nucleosome binding protein that affects chromatin structure and activity, in the hair follicle and test whether loss of HMGN1 affects the development or cycling of the follicle. We find that at the onset of hair follicle development, HMGN1 protein is expressed in the epidermal placode and in aggregated dermal fibroblasts. In the adult hair follicle, HMGN1 is specifically expressed in the basal layer of epidermis, in the outer root sheath, in the hair bulb, but not in the inner root sheath and hair shaft. The expression pattern of HMGN1 is very similar to p63, suggesting a role for HMGN1 in the transiently amplifying cells. We also find HMGN1 expression in some, but not all hair follicle stem cells as detected by its colocalization with Nestin and with BrdU label-retaining cells. The appearance of the skin and hair follicle of Hmgn1(-/-) mice was indistinguishable from that of their Hmgn1(+/+) littermates. We found that in the hair follicle the expression of HMGN2 is very similar to HMGN1 suggesting functional redundancy between these closely related HMGN variants.
authors.affiliation: Protein Section, Laboratory of Metabolism, National Cancer Institute, National Institutes of Health, Building 37, Room 3122, 9000 Rockville Pike, Bethesda, MD 20892, USA.
authors.forename: Takashi
authors.initials: T
authors.lastname: Furusawa
authors.forename: Jung-Ho
authors.initials: JH
authors.lastname: Ko
authors.forename: Yehudit
authors.initials: Y
authors.lastname: Birger
authors.forename: Michael
authors.initials: M
authors.lastname: Bustin
mesh_terms: D000818:Animals; D005455:Fluorescent Antibody Technique; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D024241:HMGN1 Protein / Q000235:genetics*; D018859:Hair Follicle / Q000196:embryology / Q000254:growth & development / Q000378:metabolism*; D017403:In Situ Hybridization; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D012867:Skin / Q000196:embryology / Q000254:growth & development / Q000378:metabolism
chemical_list: D024241:HMGN1 Protein