Transgenic RNAi-mediated reduction of LZP3 in Lagurus lagurus oocytes results in decreased fertilization ability in IVF.
Structural differences in oligosaccharides on mammalian zona pellucida 3(ZP3) from different species may determine whether or not spermatozoa being able to bind to ZP. We reported here that by microinjecting the siRNA interference recombinant construct pGenesil-ZP31 encoding a Lagurus zp3 (lzp3) hairpin dsRNA of 21 bp into the inmatured oocytes of Lagurus lagurus, distributed in northern region of Xingjiang, to disturb its fertility. Results of in vitro fertilization after in vitro maturation of the immature oocytes of Lagurus lagurus showed that the fertilization rate of the transgenic oocytes carried pGenesil-ZP31 was decreased greatly (2.82%) compared to the oocytes carried pGenesil-HK (15.71%), suggesting that the transgenic RNAi-mediated silencing of lzp3 in Lagurus lagurus oocytes results in decreased fertilization ability. These results proved that LZP3 of Lagurus lagurus, like other mammalians, is essential for the recognition between oocyte and spermatozoa.
Zhuang
Shu Zhen
SZ
Key Laboratory of Molecular Biology, College of Life Science and Technology, Xinjiang Key Laboratory of Biological Resources and Genetic Engineering, Xinjiang University, Urumqi, People's Republic of China. zhuangshuzhen@126.com
Li
Xin Jian
XJ
Zhang
Ai Lian
AL
Ma
Ji
J
Zheng
Yao Hu
YH
Zhang
Fu Chun
FC
D000818:Animals; D003411:Arvicolinae / Q000378:metabolism*; D002681:China; D017931:DNA Primers / Q000235:genetics; D004527:Egg Proteins / Q000235:genetics / Q000378:metabolism; D005306:Fertilization / Q000235:genetics*; D005307:Fertilization in Vitro; D007150:Immunohistochemistry; D055029:Inverted Repeat Sequences / Q000235:genetics; D008562:Membrane Glycoproteins / Q000172:deficiency* / Q000235:genetics / Q000378:metabolism; D008845:Microinjections; D009865:Oocytes / Q000378:metabolism*; D034622:RNA Interference; D011956:Receptors, Cell Surface / Q000172:deficiency* / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D000072216:Zona Pellucida Glycoproteins
D017931:DNA Primers; D004527:Egg Proteins; D008562:Membrane Glycoproteins; D011956:Receptors, Cell Surface; D000072216:Zona Pellucida Glycoproteins