Proteomic identification of extracellular proteins regulated by the Gna1 Galpha subunit in Stagonospora nodorum.
The fungus Stagonospora nodorum is the causal agent of stagonospora nodorum blotch (syn. leaf and glume blotch) disease of wheat. The Gna1-encoded Galpha protein is an important signal transduction component in the fungus, which is required for full pathogenicity, sporulation and extracellular depolymerase production. In this study, we sought to gain a better understanding of defects associated with the gna1 mutant by using two-dimensional gel electrophoresis to analyse the extracellular proteome for differences to the wildtype. Mass spectrometry analysis of altered abundant protein spots and peptide matching to the Stagonospora nodorum genome database have led to the identification of genes implicated in cell wall degradation, proteolysis, RNA hydrolysis and aromatic compound metabolism. In addition, quantitative RT-PCR has demonstrated that some of the encoding genes showed differential expression throughout host infection. Implications of these proteins and their corresponding genes in fungal virulence are discussed.
Tan
Kar-Chun
KC
Australian Centre for Necrotrophic Fungal Pathogens, SABC, Faculty of Health Sciences, Murdoch University, Murdoch 6150, Australia.
Heazlewood
Joshua L
JL
Millar
A Harvey
AH
Oliver
Richard P
RP
Solomon
Peter S
PS
D001203:Ascomycota / Q000235:genetics* / Q000472:pathogenicity; D015180:Electrophoresis, Gel, Two-Dimensional; D005656:Fungal Proteins / Q000235:genetics; D044385:GTP-Binding Protein alpha Subunits / Q000235:genetics*; D015966:Gene Expression Regulation, Fungal / Q000235:genetics; D013058:Mass Spectrometry; D010935:Plant Diseases / Q000235:genetics* / Q000382:microbiology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction / Q000235:genetics; D014908:Triticum / Q000382:microbiology*
D005656:Fungal Proteins; D044385:GTP-Binding Protein alpha Subunits