title: The effect of surface microtopography of poly(dimethylsiloxane) on protein adsorption, platelet and cell adhesion.
abstract: Chemical homogeneous poly(dimethylsiloxane) (PDMS) surface with dot-like protrusion pattern was used to investigate the individual effect of surface microtopography on protein adsorption and subsequent biological responses. Fibrinogen (Fg) and fibronectin (Fn) were chosen as model proteins due to their effect on platelet and cell adhesion, respectively. Fg labeled with (125)I and fluorescein isothiocyanate (FITC) was used to study its adsorption on flat and patterned surfaces. Patterned surface has a 46% increase in the adsorption of Fg when compared with flat surface. However, the surface area of the patterned surface was only 8% larger than that of the flat surface. Therefore, the increase in the surface area was not the only factor responsible for the increase in protein adsorption. Clear fluorescent pattern was visualized on patterned surface, indicating that adsorbed Fg regularly distributed and adsorbed most on the flanks and valleys of the protrusions. Such distribution and local enrichment of Fg presumably caused the specific location of platelets adhered from platelet-rich plasma (PRP) and flowing whole blood (FWB) on patterned surface. Furthermore, the different combination of surface topography and pre-adsorbed Fn could influence the adhesion of L929 cells. The flat surface with pre-adsorbed Fn was the optimum substrate while the virgin patterned surface was the poor substrate in terms of L929 cells spread.
authors.affiliation: State Key Laboratory of Advanced Technology for Materials Synthesis and Processing, Wuhan University of Technology, Wuhan 430070, PR China. hongchen@whut.edu.cn
authors.forename: Hong
authors.initials: H
authors.lastname: Chen
authors.forename: Wei
authors.initials: W
authors.lastname: Song
authors.forename: Feng
authors.initials: F
authors.lastname: Zhou
authors.forename: Zhongkui
authors.initials: Z
authors.lastname: Wu
authors.forename: He
authors.initials: H
authors.lastname: Huang
authors.forename: Junhu
authors.initials: J
authors.lastname: Zhang
authors.forename: Quan
authors.initials: Q
authors.lastname: Lin
authors.forename: Bai
authors.initials: B
authors.lastname: Yang
mesh_terms: D000327:Adsorption / Q000187:drug effects; D000818:Animals; D001792:Blood Platelets / Q000166:cytology / Q000648:ultrastructure; D002448:Cell Adhesion / Q000187:drug effects; D002452:Cell Count; D048430:Cell Shape / Q000187:drug effects; D004129:Dimethylpolysiloxanes / Q000737:chemistry* / Q000494:pharmacology*; D005340:Fibrinogen / Q000378:metabolism*; D006801:Humans; D051379:Mice; D018625:Microscopy, Atomic Force; D018613:Microscopy, Confocal; D010973:Platelet Adhesiveness / Q000187:drug effects*; D053657:Platelet-Rich Plasma; D013499:Surface Properties / Q000187:drug effects
chemical_list: D004129:Dimethylpolysiloxanes; D005340:Fibrinogen