title: Effects of a chemical chaperone on genetic mutations in alpha-galactosidase A in Korean patients with Fabry disease.
abstract: Fabry disease is an X-linked inborn error of glycosphingolipid catabolism that results from mutations in the gene encoding the alpha-galactosidase A (GLA) enzyme. We have identified 15 distinct mutations in the GLA gene in 13 unrelated patients with classic Fabry disease and 2 unrelated patients with atypical Fabry disease. Two of the identified mutations were novel (i.e., the D231G missense mutation and the L268delfsX1 deletion mutation). This study evaluated the effects of the chemical chaperones 1-deoxygalactonojirimycin (DGJ) on the function of GLA in vitro, in cells containing missense mutations in the GLA gene. Nine missense and a nonsense mutations, including one novel mutation were cloned into mammalian expression vectors. After transient expression in COS-7 cells, GLA enzyme activity and protein expression were analyzed using fluorescence spectrophotometry and Western blot analysis, respectively. DGJ enhanced GLA enzyme activity in the M42V, I91T, R112C and F113L mutants. Interestingly, the I91T and F113L mutations are associated with the atypical form of Fabry disease. However, DGJ treatment did not have any significant effect on the GLA enzyme activity and protein expression of other mutants, including C142W, D231G, D266N, and S297F. Of note, GLA enzyme activity was not detected in the novel mutant (i.e., D231G), although protein expression was similar to the wild type. In the absence of DGJ, the E66Q mutant had wild-type levels of GLA protein expression and approximately 40% GLA activity, indicating that E66Q is either a mild mutation or a functional single nucleotide polymorphism (SNP). Thus, the results of this study suggest that the chemical chaperone DGJ enhances GLA enzyme activity and protein expression in milder mutations associated with the atypical form of Fabry disease.
authors.affiliation: Genome Research Center for Birth Defects and Genetic Disorders, University of Ulsan College of Medicine, Seoul 138-736, Korea.
authors.forename: Jung Young
authors.initials: JY
authors.lastname: Park
authors.forename: Gu Hwan
authors.initials: GH
authors.lastname: Kim
authors.forename: Sung Su
authors.initials: SS
authors.lastname: Kim
authors.forename: Jung Min
authors.initials: JM
authors.lastname: Ko
authors.forename: Jin Joo
authors.initials: JJ
authors.lastname: Lee
authors.forename: Han Wook
authors.initials: HW
authors.lastname: Yoo
mesh_terms: D017485:1-Deoxynojirimycin / Q000031:analogs & derivatives* / Q000378:metabolism; D000293:Adolescent; D000328:Adult; D000818:Animals; D044466:Asian People / Q000235:genetics*; D019556:COS Cells; D002522:Chlorocebus aethiops; D000795:Fabry Disease / Q000201:enzymology* / Q000235:genetics; D015870:Gene Expression; D006801:Humans; D008297:Male; D008875:Middle Aged; D009154:Mutation; D055815:Young Adult; D000519:alpha-Galactosidase / Q000235:genetics* / Q000378:metabolism*
chemical_list: D017485:1-Deoxynojirimycin; C090092:migalastat; D000519:alpha-Galactosidase