Bcl-10 protein highly correlates with the expression of phosphorylated p65 NF-kappaB in peripheral T-cell lymphomas and is associated with clinical outcome.
OBJECTIVE
In T cells, protein kinase C (PKC) theta plays a major role in T-cell receptor (TCR)-mediated activation of a novel nuclear factor (NF)-kappaB pathway that involves phosphorylation of p65 at serine 536 (Pp65(Ser536)). Bcl-10 acts along the same pathway downstream of PKC theta to activate NF-kappaB. The aim was to investigate the relationship between the expression of PKC theta, Bcl-10 and P-p65(Ser536) proteins and their prognostic significance in peripheral T-cell lymphomas (PTCLs).


RESULTS
Paraffin-embedded tissues from 30 patients with PTCLs treated with curative intention were evaluated retrospectively. Expression of PKC theta, Bcl-10 and P-p65(Ser536) proteins was assessed using immunohistochemistry. Expression of PKC theta was detected in 22 of 30 cases (73%), Bcl-10 in 20 of 30 (67%) and P-p65(Ser536) in 21 of 30 (70%). Bcl-10+ tumours were associated with PKC theta (18 of 22) (P < 0.0001) and Pp65Ser536 (19 of 21) expression (P < 0.0001). Patients with Bcl-10+ or P-p65(Ser536+) tumours fared better, with a 5-year overall survival of 48 and 45%, respectively, versus 0% for negative tumours (P = 0.029 and P = 0.04, respectively).


CONCLUSIONS
Bcl-10 is expressed in PTCLs, correlates with PKC theta and Pp65(Ser536) expression and seems to be associated with better survival.
Briones
Javier
J
Departments of Clinical Haematology and Pathology, Hospital de la Santa Creu i Sant Pau, Barcelona, Spain. jbriones@santpau.cat
Moga
Esther
E
Espinosa
Iñigo
I
Vergara
Carmen
C
Alvarez
Eva
E
Villa
Juliana
J
Bordes
Ramón
R
Delgado
Julio
J
Prat
Jaime
J
Sierra
Jorge
J
D048868:Adaptor Proteins, Signal Transducing / Q000378:metabolism*; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000074018:B-Cell CLL-Lymphoma 10 Protein; D014408:Biomarkers, Tumor / Q000378:metabolism*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D007527:Isoenzymes / Q000378:metabolism; D016411:Lymphoma, T-Cell, Peripheral / Q000378:metabolism* / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D010766:Phosphorylation; D011379:Prognosis; D011493:Protein Kinase C / Q000378:metabolism; D000074121:Protein Kinase C-theta; D012189:Retrospective Studies; D012694:Serine / Q000737:chemistry; D015996:Survival Rate; D046888:Tissue Array Analysis; D051996:Transcription Factor RelA / Q000737:chemistry / Q000378:metabolism*; D055815:Young Adult
D048868:Adaptor Proteins, Signal Transducing; D000074018:B-Cell CLL-Lymphoma 10 Protein; C118591:BCL10 protein, human; D014408:Biomarkers, Tumor; D007527:Isoenzymes; C498143:RELA protein, human; D051996:Transcription Factor RelA; D012694:Serine; C076233:PRKCQ protein, human; D011493:Protein Kinase C; D000074121:Protein Kinase C-theta