The effects of Scutellaria baicalensis extract on embryonic development in mice.
BACKGROUND
Scutellaria baicalensis is a perennial herbaceous plant widely distributed in Oriental areas. Its roots, a commonly used medicinal source, reputedly calm fetuses in pregnant women; however, there is no sufficient evidence to date to assess its safety during pregnancy. This study aims to evaluate the effects of S. baicalensis aqueous extract on embryonic development in ICR mice.


METHODS
Aqueous extract of S. baicalensis roots was prepared in accordance with clinical application. Pregnant mice were randomly divided into four groups, i.e., mice treated by gavage with water as negative control, with aqueous extract of 2 (1.8 times of human daily dose), 8 or 32 g/kg/day from gestation day (Gd) 6 to 15 as low-, middle-, and high-dose groups, respectively. The parameters of live and dead fetuses, resorptions, external and skeletal malformed fetuses, maternal body weight, maternal liver, kidneys, and heart weights were evaluated on Gd 18.


RESULTS
There was no significant difference in fetal parameters among four groups. Maternal absolute liver and kidneys weights in the high-dose group were significantly higher than those in negative control (p<0.05). Relative liver and kidneys weights in this group were significantly higher than those in any other group (p<0.05).


CONCLUSIONS
Oral administration of aqueous extract of S. baicalensis roots at or below 32 g/kg/day to ICR mice during organogenesis did not cause significant fetal external or skeletal malformations. However, 32 g/kg/day presented potential maternal toxicity.
Tian
Xiao Ying
XY
School of Chinese Medicine, Hong Kong Baptist University, Kowloon Tong, Hong Kong.
Cheung
Lok Man
LM
Leung
Kevin
K
Qi
Chen
C
Deng
Bin
B
Deng
Ping Xiang
PX
Xu
Min
M
D000818:Animals; D001835:Body Weight / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004365:Drugs, Chinese Herbal / Q000494:pharmacology / Q000633:toxicity*; D047108:Embryonic Development / Q000187:drug effects*; D005260:Female; D005333:Fetus / Q000187:drug effects; D006529:Hepatomegaly / Q000139:chemically induced; D007668:Kidney / Q000187:drug effects; D008099:Liver / Q000187:drug effects; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D009929:Organ Size / Q000187:drug effects; D038081:Organogenesis / Q000187:drug effects*; D008517:Phytotherapy / Q000009:adverse effects; D010936:Plant Extracts / Q000494:pharmacology / Q000633:toxicity*; D011247:Pregnancy; D011248:Pregnancy Complications / Q000139:chemically induced; D011897:Random Allocation; D031364:Scutellaria baicalensis / Q000633:toxicity*; D013045:Species Specificity
D004365:Drugs, Chinese Herbal; D010936:Plant Extracts; C499814:Scutellaria baicalensis extract