Populations of heavy fuel oil-degrading marine microbial community in presence of oil sorbent materials.
OBJECTIVE
To investigate the feasibility of applying sorbent material X-Oil in marine oil spill mitigation and to survey the interactions of oil, bacteria and sorbent.


RESULTS
In a series of microcosms, 25 different treatments including nutrient amendment, bioaugmentation with Alcanivorax borkumensis and application of sorbent were tested. Microbial community dynamics were analysed by DNA fingerprinting methods, RISA and DGGE. Results of this study showed that the microbial communities in microcosms with highly active biodegradation were strongly selected in favour of A. borkumensis. Oxygen consumption measurements in microcosms and gas chromatography of oil samples indicated the fast and intense depletion of linear alkanes as well as high oxygen consumption within 1 week followed by consequent slower degradation of branched and polyaromatic hydrocarbons.


CONCLUSIONS
Under given conditions, A. borkumensis was an essential organism for biodegradation, dominating the biofilm microbial community formation and was the reason of emulsification.


CONCLUSIONS
This study strongly emphasizes the pivotal importance of A. borkumensis as an essential organism in the initial steps of marine hydrocarbon degradation. Interaction with the sorbent material X-Oil proved to be neutral to beneficial for biodegradation and also promoted the growth of yet unknown micro-organisms.
Gertler
C
C
Department of Environmental Microbiology, HZI-Helmholtz Centre for Infection Research, Braunschweig, Germany. c.gertler@bangor.ac.uk
Gerdts
G
G
Timmis
K N
KN
Yakimov
M M
MM
Golyshin
P N
PN
D053521:Alcanivoraceae / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D001419:Bacteria / Q000235:genetics / Q000302:isolation & purification*; D001673:Biodegradation, Environmental; D002849:Chromatography, Gas; D016172:DNA Fingerprinting; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D005648:Fuel Oils / Q000382:microbiology; D006838:Hydrocarbons / Q000378:metabolism*; D010100:Oxygen / Q000378:metabolism; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012623:Seawater / Q000382:microbiology; D017422:Sequence Analysis, DNA; D014871:Water Microbiology; D014874:Water Pollutants, Chemical / Q000378:metabolism*
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005648:Fuel Oils; D006838:Hydrocarbons; D012336:RNA, Ribosomal, 16S; D014874:Water Pollutants, Chemical; D010100:Oxygen