title: Iterative in situ click chemistry creates antibody-like protein-capture agents.
abstract: Special agents for protein capture: Iterative in situ click chemistry (see scheme for the tertiary ligand screen) and the one-bead-one-compound method for the creation of a peptide library enable the fragment-based assembly of selective high-affinity protein-capture agents. The resulting ligands are water-soluble and stable chemically, biochemically, and thermally. They can be produced in gram quantities through copper(I)-catalyzed cycloaddition.
authors.affiliation: Division of Chemistry and Chemical Engineering, California Institute of Technology, 1200 East California Boulevard, Pasadena, CA 91125, USA.
authors.forename: Heather D
authors.initials: HD
authors.lastname: Agnew
authors.forename: Rosemary D
authors.initials: RD
authors.lastname: Rohde
authors.forename: Steven W
authors.initials: SW
authors.lastname: Millward
authors.forename: Arundhati
authors.initials: A
authors.lastname: Nag
authors.forename: Woon-Seok
authors.initials: WS
authors.lastname: Yeo
authors.forename: Jason E
authors.initials: JE
authors.lastname: Hein
authors.forename: Suresh M
authors.initials: SM
authors.lastname: Pitram
authors.forename: Abdul Ahad
authors.initials: AA
authors.lastname: Tariq
authors.forename: Vanessa M
authors.initials: VM
authors.lastname: Burns
authors.forename: Russell J
authors.initials: RJ
authors.lastname: Krom
authors.forename: Valery V
authors.initials: VV
authors.lastname: Fokin
authors.forename: K Barry
authors.initials: KB
authors.lastname: Sharpless
authors.forename: James R
authors.initials: JR
authors.lastname: Heath
mesh_terms: D000906:Antibodies / Q000737:chemistry; D002384:Catalysis; D003300:Copper / Q000737:chemistry; D008024:Ligands; D019151:Peptide Library; D010455:Peptides / Q000737:chemistry; D011485:Protein Binding; D011506:Proteins / Q000737:chemistry*; D014230:Triazoles / Q000737:chemistry*
chemical_list: D000906:Antibodies; D008024:Ligands; D019151:Peptide Library; D010455:Peptides; D011506:Proteins; D014230:Triazoles; D003300:Copper