Immobilization of Trichoderma viride for enhanced methylene blue biosorption: batch and column studies.
An efficient dye biosorbent was developed by entrapping a fungus mold, Trichoderma viride, within loofa sponge (LS) matirx. Immobilization enhanced the sorption of dye by 30% at equilibrium as compared with T. viride free biomass (TVFB). The maximum dye biosorption capacity of T. viride immobilized onto loofa sponge (TVILS) and TVFB was found to be 201.52 and 155.06 mg g(-1) biomass, respectively. The kinetics of dye removal by TVILS was rapid, with 84.3% sorption within the first 30 min and equilibrium after 90 min, whereas sorption by TVFB was slower as 61.4% dye was removed in first 30 min and equilibrium was achieved in 120 min. Biosorption kinetics and equilibria followed the pseudo-second-order and Langmuir adsorption models. FTIR spectroscopy of T. viride biomass showed that amine, hydroxyl, carbonyl and amide bonds were involved in the sorption of dye. Dye desorption from dye-laden TVILS with 0.1M HCl was 99%. Regenerated TVILS was reusable without any appreciable decrease in its biosorption capacity during five repeated cycles. The dye removing capacity of TVILS in a continuous-flow column bioreactor was better than in batch-scale procedures. The study shows that TVILS has the potential of application as an efficient biosorbent for the removal of methylene blue from aqueous solutions.
Saeed
Asma
A
Biotechnology Group, Centre for Environment Protection Studies, PCSIR Laboratories Complex, Lahore, Pakistan.
Iqbal
Muhammad
M
Zafar
Saeed Iqbal
SI
D000327:Adsorption; D001673:Biodegradation, Environmental; D018533:Biomass; D007220:Industrial Waste; D007700:Kinetics; D008751:Methylene Blue / Q000378:metabolism*; D014242:Trichoderma / Q000378:metabolism*; D014874:Water Pollutants, Chemical / Q000378:metabolism*
D007220:Industrial Waste; D014874:Water Pollutants, Chemical; D008751:Methylene Blue