[Quantitative evaluation of residual endodontic microorganisms after mechanical root canal preparation different chemical preparations by real-time PCR].
OBJECTIVE
To establish a quick, sensitive method for quantifying root canal flora and investigate the effects of different root canal preparations on the pathogenic bacteria at RNA level.


METHODS
A total of 24 single-rooted teeth with chronic apical periodontitis were selected and prepared using 3% H2O2 combined with 1% NaClO, EDTA combined with 3% H(2)O(2),1% NaClO, respectively,the samples were taken before and after root canal preparation. After isolation of total RNA from the root canal samples, cDNA was synthesized by reverse transcription, and detected by real-time PCR. The data were analyzed with SAS 6.12 software package.


RESULTS
The number of bacteria in the root canal reduced dramatically after mechanical preparation and irrigated using 3% H(2)O(2) and 1% NaClO(P<0.01). Further combined with EDTA, its effect was better than that of simply irrigated using 3% H(2)O(2) and 1% NaClO(P<0.05).


CONCLUSIONS
Real-time PCR can be employed in the identification of bacteria flora in the root canal, both methods of root canal preparation can effectively reduce the number of bacteria flora.
Jiang
Yun-tao
YT
Department of Endodontics and Operative Dentistry, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, China. jiangyuntao1974@163.com
Xia
Wen-wei
WW
Liang
Jing-ping
JP
Ma
Shan-fen
SF
Yan
Pei-fang
PF
D003786:Dental Pulp Cavity; D006801:Humans; D006861:Hydrogen Peroxide; D010485:Periapical Periodontitis; D060888:Real-Time Polymerase Chain Reaction; D012388:Root Canal Irrigants; D018915:Root Canal Preparation; D012390:Root Canal Therapy
D012388:Root Canal Irrigants; D006861:Hydrogen Peroxide