Molecular cloning of a dominant roller mutant and establishment of DNA-mediated transformation in the nematode Pristionchus pacificus.
We report the molecular cloning of a dominant Roller mutant of Pristionchus pacificus, which encodes a cuticle collagen. We use the mutant locus as a marker to develop transgenic technique by generating complex arrays and present flourescent-protein based transcriptional reporter constructs for P. pacificus.
Schlager
Benjamin
B
Department for Evolutionary Biology, Max-Planck-Institute for Developmental Biology, Tübingen, Germany.
Wang
Xiaoyue
X
Braach
Georg
G
Sommer
Ralf J
RJ
D000595:Amino Acid Sequence; D000818:Animals; D003001:Cloning, Molecular; D003094:Collagen / Q000235:genetics; D018509:DNA, Helminth / Q000235:genetics*; D005799:Genes, Dominant; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D015801:Helminth Proteins / Q000235:genetics; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D009154:Mutation; D009348:Nematoda / Q000235:genetics*; D010957:Plasmids / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D014170:Transformation, Genetic; D019076:Transgenes / Q000235:genetics; D000097573:Red Fluorescent Protein
D003094:Collagen; D018509:DNA, Helminth; D049452:Green Fluorescent Proteins; D015801:Helminth Proteins; D008164:Luminescent Proteins; D011993:Recombinant Fusion Proteins; D000097573:Red Fluorescent Protein