Immobilization of bioluminescent Escherichia coli cells using natural and artificial fibers treated with polyethyleneimine.
Biosensors based on whole-cell bioluminescence have the potential to become a cost-effective alternative to conventional detection methods upon validation of target selectivity and sensitivity. However, quantitative analysis of bioluminescence is greatly hindered due to lack of control over the total number of cells in a suspending culture. In this study, the effect of surface properties of genetically engineered luminous E. coli cells and fibrous matrices on the immobilization capacity and effectiveness under various environmental conditions were characterized. Four different fibers, including cotton, polyester, viscose rayon, and silk, were investigated. Although cell adhesion was observed on untreated viscose and cotton fibers, viscose fiber pretreated with 0.667% polyethyleneimine (PEI) was found capable of immobilizing the most viable E. coli DPD2234 cells, followed by viscose treated with 0.33% and 1% PEI. The cells immobilized on PEI-treated viscose remained viable and yielded 20% or more bioluminescence signals immediately upon contact with the inducer up to 72 h without feeding nutrients to the cells, suggesting that viscose treated with 0.667% PEI could provide a stable immobilization mechanism for bioluminescent E. coli cells with long sensing period, quick response time, and good signal reproducibility.
Chu
Yi-Fang
YF
Department of Animal and Food Sciences, University of Delaware, Newark, DE 19717, USA.
Hsu
Chia-Hua
CH
Soma
Pavan K
PK
Lo
Y Martin
YM
D001422:Bacterial Adhesion; D015374:Biosensing Techniques; D001709:Biotechnology / Q000379:methods*; D003432:Cross-Linking Reagents / Q000737:chemistry; D004269:DNA, Bacterial / Q000235:genetics / Q000494:pharmacology; D004867:Equipment Design; D004926:Escherichia coli / Q000378:metabolism*; D049449:Luminescence; D008163:Luminescent Measurements / Q000379:methods; D008855:Microscopy, Electron, Scanning / Q000379:methods; D015233:Models, Statistical; D011094:Polyethyleneimine / Q000737:chemistry*; D012680:Sensitivity and Specificity; D013499:Surface Properties; D013997:Time Factors
D003432:Cross-Linking Reagents; D004269:DNA, Bacterial; D011094:Polyethyleneimine