title: Formulation and immunological evaluation of novel vaccine delivery systems based on bioresorbable poly(ethylene glycol)-block-poly(lactide-co-epsilon-caprolactone).
abstract: Novel emulsion-type vaccine delivery systems based on the amphiphilic bioresorbable polymer poly(ethylene glycol)-block-poly(lactide-co-epsilon-caprolactone) (PEG-b-PLACL) and selected oils were developed here. Physicochemical characterizations such as stability, a droplet test, microscopic aspects, and in vitro release showed that PEG-b-PLACL-emulsified formulations have several advantages over traditional vaccine adjuvants in that they are stable, reproducible, and homogeneous fine particles with an appropriate size to facilitate the induction of potent immune responses. Different dispersion-type emulsions have provided different release profiles using ovalbumin in model studies. Immunogenicity studies in mice have shown that antigen-specific antibody titers and T-cell proliferative responses, as well as the secretion of IFN-gamma, were significantly enhanced for ovalbumin after formulation with PEG-b-PLACL-based emulsions. These features are of great interest for applications in delivery systems of prophylactic and therapeutic vaccine candidates.
authors.affiliation: Vaccine Research and Development Center, National Health Research Institutes, Zhunan, Miaoli 35053, Taiwan.
authors.forename: Ming-Hsi
authors.initials: MH
authors.lastname: Huang
authors.forename: Ai-Hsiang
authors.initials: AH
authors.lastname: Chou
authors.forename: Shu-Pei
authors.initials: SP
authors.lastname: Lien
authors.forename: Hsin-Wei
authors.initials: HW
authors.lastname: Chen
authors.forename: Chiung-Yi
authors.initials: CY
authors.lastname: Huang
authors.forename: Wei-Wen
authors.initials: WW
authors.lastname: Chen
authors.forename: Pele
authors.initials: P
authors.lastname: Chong
authors.forename: Shih-Jen
authors.initials: SJ
authors.lastname: Liu
authors.forename: Chih-Hsiang
authors.initials: CH
authors.lastname: Leng
mesh_terms: D000818:Animals; D016503:Drug Delivery Systems; D004655:Emulsions; D005260:Female; D006801:Humans; D007107:Immune System; D007074:Immunoglobulin G / Q000737:chemistry; D051379:Mice; D008807:Mice, Inbred BALB C; D009821:Oils / Q000737:chemistry; D010316:Particle Size; D011091:Polyesters / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry; D014612:Vaccines / Q000008:administration & dosage*
chemical_list: D004655:Emulsions; D007074:Immunoglobulin G; D009821:Oils; D011091:Polyesters; D011108:Polymers; D014612:Vaccines; D011092:Polyethylene Glycols; C062968:lactide-caprolactone copolymer