Ameliorative effect of 1,2-benzenedicarboxylic acid dinonyl ester against amyloid beta peptide-induced neurotoxicity.
Amyloid beta peptide (Abeta)-induced oxidative stress may be linked to neurodegenerative disease. Ethanol extracts of Rosa laevigata protected PC12 cells from hydrogen peroxide-induced oxidative stress. (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) reduction assays revealed a significant increase in cell viability when oxidatively stressed PC12 cells were treated with R. laevigata extract. The effect of R. laevigata on oxidative stress-induced cell death was further investigated by lactate dehydrogenase release assays and trypan blue exclusion assays. Administration of 1,2-benzenedicarboxylic acid dinonyl ester from R. laevigata extract to mice infused with Abeta significantly reversed learning and memory impairment in behavioural tests. After behavioural testing, the mice were sacrificed and brains were collected for the examination of lipid peroxidation, catalase activity and acetylcholinesterase (AchE) activity. These results suggest that 1,2-benzenedicarboxylic acid dinonyl ester from R. laevigata extract may be able to reduce Abeta-induced neurotoxicity, possibly by reducing oxidative stress. Therefore, R. laevigata extract may be useful for the prevention of oxidative stress-induced neurodegenerative disorders.
Jung Choi
Soo
S
Department of Food Technology, Korea University, Seoul, Korea.
Kim
Mi Jeong
MJ
Jin Heo
Ho
H
Kim
Jae Kyeum
JK
Jin Jun
Woo
W
Kim
Hye Kyung
HK
Kim
Eun-Ki
EK
Ok Kim
Myeong
M
Yon Cho
Hong
H
Hwang
Han-Joon
HJ
Jun Kim
Young
Y
Shin
Dong-Hoon
DH
D016564:Amyloid beta-Protein Precursor / Q000633:toxicity*; D000818:Animals; D001362:Avoidance Learning / Q000187:drug effects; D016923:Cell Death / Q000187:drug effects; D002462:Cell Membrane / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002800:Cholinesterase Inhibitors / Q000494:pharmacology; D006861:Hydrogen Peroxide / Q000633:toxicity; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D018782:Maze Learning / Q000187:drug effects; D051379:Mice; D008813:Mice, Inbred ICR; D020258:Neurotoxicity Syndromes / Q000378:metabolism / Q000517:prevention & control*; D018384:Oxidative Stress / Q000187:drug effects; D016716:PC12 Cells; D010795:Phthalic Acids / Q000494:pharmacology / Q000627:therapeutic use*; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology; D051381:Rats; D027826:Rosa / Q000737:chemistry
D016564:Amyloid beta-Protein Precursor; D002800:Cholinesterase Inhibitors; D010795:Phthalic Acids; D010936:Plant Extracts; C019174:dinonylphthalate; D006861:Hydrogen Peroxide