Effect of gelatin hydrogel incorporating fibroblast growth factor 2 on human meniscal cells in an organ culture model.
Efforts to use growth factors to enhance the healing potential of the meniscus have been impeded because their half-lives are too short to maintain the biological activity. The thread was coated with gelatin hydrogel and fibroblast growth factor 2 (FGF 2) was biologically stabilized by incorporating in a gelatin hydrogel-coated thread. The purpose of this study is to investigate the effect of gelatin hydrogel-coated thread incorporating FGF 2 on human meniscal cells in an organ culture. Twenty-five menisci were cut into small pieces, and selected pieces were sutured with gelatin hydrogel-coated thread incorporating FGF 2 (FGF(+) group) or physiologic saline (FGF(-) group), followed by organ culture. The meniscal samples histologically evaluated 4, 7, and 14 days later. The cell density and the number of PCNA-positive cells for the FGF(+) group were higher than those of the FGF(-) group, while the number of TUNEL-positive cells was lower. These results suggest that FGF 2 stimulates the proliferation of meniscal cells and inhibits meniscal cell death. Gelatin hydrogel-coated threads releasable FGF 2 may be useful to promote repairing of human meniscus.
Narita
Atsushi
A
Department of Orthopaedic Surgery, Yamagata University School of Medicine, Iida-Nishi, Yamagata, Japan. anarita@med.id.yamagata-u.ac.jp
Takahara
Masatoshi
M
Ogino
Toshihiko
T
Fukushima
Shigenobu
S
Kimura
Yu
Y
Tabata
Yasuhiko
Y
D000368:Aged; D000369:Aged, 80 and over; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D019902:Chondrocytes / Q000187:drug effects*; D004343:Drug Implants; D005260:Female; D016222:Fibroblast Growth Factor 2 / Q000008:administration & dosage*; D006801:Humans; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D020287:In Situ Nick-End Labeling; D036341:Intercellular Signaling Peptides and Proteins / Q000008:administration & dosage*; D008297:Male; D008592:Menisci, Tibial / Q000166:cytology / Q000187:drug effects*; D009924:Organ Culture Techniques; D013537:Sutures
D004343:Drug Implants; D036341:Intercellular Signaling Peptides and Proteins; D016222:Fibroblast Growth Factor 2; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate