The NER proteins are differentially expressed in ever smokers and in never smokers with lung adenocarcinoma.
BACKGROUND
The expression levels of excision repair cross-complementation group 1 (ERCC1), replication protein A (RPA) and xeroderma pigmentosum group F (XPF) nucleotide excision repair proteins may be important in the response to platin-based therapy in lung cancer patients. It is not known whether ERCC1, RPA and XPF expression levels differ between ever smokers (ES) and never smokers (NS).


METHODS
ERCC1, RPA and XPF expression levels were immunohistochemically evaluated in 125 patients with resected lung adenocarcinoma (AC) and carefully reviewed smoking status.


RESULTS
ERCC1 was correlated with XPF (P = 0.001), but not with RPA (P = 0.11). In the univariate analysis, ERCC1 and XPF levels were higher in NS compared with ES (P = 0.004 and P = 0.003, respectively). In the multivariate analysis, the smoking status was predictive of the ERCC1 level [odds ratio (OR) 2.5, 95% confidence interval (CI) 1.03-6.2] after adjustment for variables linked to the smoking status, including age and the presence of bronchioloalveolar (BAC) features. The smoking status was also predictive of both RPA (OR 6.7, 95% CI 1.5-33.3) and XPF levels (OR 12.5, 95% CI 2.9-50) after adjusting for age, sex and BAC features.


CONCLUSIONS
In patients with resected lung AC, ERCC1, RPA and XPF expression levels are higher in NS compared with ES.
Planchard
D
D
Medical Oncology and Translational Research, Institut de Cancérologie Gustave Roussy,Villejuif, France.
Domont
J
J
Taranchon
E
E
Monnet
I
I
Tredaniel
J
J
Caliandro
R
R
Validire
P
P
Besse
B
B
Soria
J-C
JC
Fouret
P
P
D000230:Adenocarcinoma / Q000378:metabolism* / Q000473:pathology; D000368:Aged; D015331:Cohort Studies; D004268:DNA-Binding Proteins / Q000378:metabolism; D004720:Endonucleases / Q000378:metabolism; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D016015:Logistic Models; D008175:Lung Neoplasms / Q000378:metabolism* / Q000473:pathology; D008297:Male; D046228:Microarray Analysis; D008875:Middle Aged; D015999:Multivariate Analysis; D016016:Proportional Hazards Models; D051716:Replication Protein A / Q000378:metabolism; D017678:Sex Distribution; D012907:Smoking / Q000009:adverse effects* / Q000378:metabolism
D004268:DNA-Binding Proteins; D051716:Replication Protein A; C095888:xeroderma pigmentosum group F protein; C071447:ERCC1 protein, human; D004720:Endonucleases