Histoplasma capsulatum var. duboisii infection in a patient with AIDS: rapid diagnosis using polymerase chain reaction-sequencing.
We describe an original case of disseminated infection with Histoplasma capsulatum (Hc) var. duboisii in an African patient with AIDS who migrated to Switzerland. The diagnosis of histoplasmosis was suggested using direct examination of tissues and confirmed in 24 h with a panfungal polymerase chain reaction assay. The variety duboisii of Hc was established using DNA sequencing of the polymorphic genomic region OLE. Molecular tools allow diagnosis of histoplasmosis in 24 h, which is drastically shorter than culture procedures.
Pellaton
Cyril
C
Infectious Diseases Service, Centre Hospitalier Universitaire Vaudois, University of Lausanne, 1011 Lausanne, Switzerland.
Cavassini
Matthias
M
Jaton-Ogay
Katia
K
Carron
Pierre-Nicolas
PN
Christen-Zaech
Stéphanie
S
Calandra
Thierry
T
Bille
Jacques
J
Hauser
Philippe M
PM
D000163:Acquired Immunodeficiency Syndrome / Q000150:complications*; D000328:Adult; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D054242:Emigrants and Immigrants; D006658:Histoplasma / Q000235:genetics / Q000302:isolation & purification*; D006660:Histoplasmosis / Q000175:diagnosis*; D006801:Humans; D008853:Microscopy / Q000379:methods; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods; D017422:Sequence Analysis, DNA; D013557:Switzerland
D004271:DNA, Fungal