A novel nitroreductase of Staphylococcus aureus with S-nitrosoglutathione reductase activity.
In this report we show that inactivation of the putative nitroreductase SA0UHSC_00833 (ntrA) increases the sensitivity of Staphylococcus aureus to S-nitrosoglutathione (GSNO) and augments its resistance to nitrofurans. S. aureus NtrA is a bifunctional enzyme that exhibits nitroreductase and GSNO reductase activity. A phylogenetic analysis suggests that NtrA is a member of a novel family of nitroreductases that seems to play a dual role in vivo, promoting nitrofuran activation and protecting the cell against transnitrosylation.
Tavares
Ana Filipa N
AF
Instituto de Tecnologia Química e Biológica, UNL, Av. da República (EAN), 2780-157 Oeiras, Portugal.
Nobre
Lígia S
LS
Melo
Ana M P
AM
Saraiva
Lígia M
LM
D000445:Aldehyde Oxidoreductases / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D009154:Mutation; D009581:Nitrofurans / Q000494:pharmacology; D009601:Nitroreductases / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D020411:Oligonucleotide Array Sequence Analysis; D010802:Phylogeny; D013211:Staphylococcus aureus / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics
D009581:Nitrofurans; D000445:Aldehyde Oxidoreductases; C087006:formaldehyde dehydrogenase, glutathione-independent; D009601:Nitroreductases