Cuticular lipids of female solitary bees, Osmia lignaria Say and Megachile rotundata (F.) (Hymenoptera: Megachilidae).
The cuticular lipids of the cavity-nesting adult female solitary bees, Osmia lignaria Say and Megachile rotundata (F.) (Hymenoptera: Megachilidae), were analyzed by gas chromatography (GC) and combined GC-mass spectrometry. The cuticular lipids of these female bees are mainly consisted of hydrocarbons. For O. lignaria, nearly 64% of the cuticular lipids were C(25)-C(31) mono-alkenes. For M. rotundata, 48% of the cuticular lipids were C(23)-C(33) alkanes with nearly the same quantities of the same chain-length mono-alkenes (45%). For the mono-alkenes of O. lignaria, 14 mono-alkene constituents were identified, with two of these, 9-heptacosene and 7-nonacosene, comprising 67% of the total alkene distribution. For M. rotundata females, the mixtures of mono-alkenes were more complex with 26 constituents identified and quantified. For the M. rotundata mono-alkenes, 57% of the total composition consisted of the three alkenes, 7-pentacosene, 9-pentacosene and 7-heptacosene. For both bee species, small quantities of C(40)-C(48) wax esters were also characterized with the major components possessing a C(18) mono-unsaturated fatty acid (9-octadecenoate) moiety esterified to even-carbon number (C(22-30)) fatty alcohols. The possible role of these cuticular lipids as nest recognition chemicals is discussed in light of nesting behavior of managed crop pollinators.
Buckner
James S
JS
Red River Valley Agricultural Research Center, USDA-ARS, Fargo, ND 58105, USA. james.buckner@ars.usda.gov
Pitts-Singer
Theresa L
TL
Guédot
Christelle
C
Hagen
Marcia M
MM
Fatland
Charlotte L
CL
Kemp
William P
WP
D000818:Animals; D001516:Bees / Q000378:metabolism*; D004952:Esters / Q000032:analysis; D005227:Fatty Acids / Q000378:metabolism*; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry; D006838:Hydrocarbons / Q000032:analysis; D014885:Waxes / Q000032:analysis
D004952:Esters; D005227:Fatty Acids; D006838:Hydrocarbons; D014885:Waxes