title: Characterization of enzymatic hydrolyzed snow crab (Chionoecetes opilio) by-product fractions: a source of high-valued biomolecules.
abstract: Snow crab (Chionoecetes opilio) constitutes valuable and nutritional sources of components, such as proteins, lipids and chitin. The present investigation was undertaken to evaluate the feasibility of applying a pilot scale enzymatic hydrolysis process of snow crab by-products, followed by fractionation, in order to recover enriched high-valued compounds. The yield of snow crab by-products recovered after manual processing; on a dry weight was 87.4%. The by-products (raw materials) were mainly moist (approximately 78%), and contained 42.9% proteins, 14.8% lipids, 25.7% minerals, 16.2% chitin, all expressed on a dry weight. The fatty acid profile of snow crab by-products and all fractions obtained following processing showed a higher content in mono-unsaturated fatty acids (MUFAs; approximately 50%), followed by polyunsaturated fatty acids (PUFAs; approximately 20%) and saturated fatty acids (SFAs; approximately 15%). The n-3/n-6 ratio was approximately 10 and represents a good index of nutritional value for snow crab oil by-products. Most protein enriched fractions demonstrate a well-balanced amino acid composition, notably the most essential amino acids. These protein fractions are characterized by biomolecules having a relatively low molecular weight (35 kDa and less) range. The enzymatic hydrolysis process developed in this study shows that snow crab by-products should to be viewed as having the potential of being identified as high-valued products. Even though the process could be optimized, it is controllable, and depending on hydrolyses conditions, the products obtained are reproducible and well defined. Results presented in this study indicate that snow crab by-products may serve as excellent nutritional components for future applications in the health and food sectors.
authors.affiliation: Department of Biology, Chemistry and Geography, Université du Québec à Rimouski, Rimouski, Québec, Canada. lucie_beaulieu@uqar.qc.ca
authors.forename: Lucie
authors.initials: L
authors.lastname: Beaulieu
authors.forename: Jacinthe
authors.initials: J
authors.lastname: Thibodeau
authors.forename: Piotr
authors.initials: P
authors.lastname: Bryl
authors.forename: Marie-Elise
authors.initials: ME
authors.lastname: Carbonneau
mesh_terms: D000596:Amino Acids / Q000032:analysis / Q000737:chemistry; D000818:Animals; D001709:Biotechnology / Q000379:methods; D002138:Calibration; D002686:Chitin / Q000032:analysis*; D002845:Chromatography / Q000379:methods; D003445:Crustacea / Q000737:chemistry*; D004798:Enzymes / Q000737:chemistry*; D005227:Fatty Acids / Q000032:analysis; D006868:Hydrolysis; D008055:Lipids / Q000032:analysis* / Q000737:chemistry; D008970:Molecular Weight
chemical_list: D000596:Amino Acids; D004798:Enzymes; D005227:Fatty Acids; D008055:Lipids; D002686:Chitin