title: Solid substrate-room temperature phosphorimetry for the determination of residual clenbuterol hydrochloride based on the catalysis of sodium periodate oxidizing eosine Y.
abstract: Clenbuterol hydrochloride (CLB) could catalyze NaIO(4) oxidation of eosine Y (R), which caused the room temperature phosphorescence (RTP) signal of R to quench sharply. The DeltaI(P) (= I(P2)-I(P1), I(P2) was RTP intensities of reagent blank and I(P1) was RTP intensities of test solution) of the system was directly proportional to the content of CLB. According to that academic thought, a new solid substrate-room temperature phosphorimetry (SS-RTP) for the determination of trace CLB has been established. This method has high sensitivity (detection limit (LD): 0.021 zg spot(-1), corresponding concentration: 5.2x10(-20) g mL(-1)) and good selectivity (Er = +/-5%, interfering species were of no interference). It has been applied to the determination of residual CLB in the practical samples. The results were verified using HPLC and GC/MS methods. The reaction mechanism of catalytic SS-RTP for the determination of residual CLB was also discussed.
authors.affiliation: Department of Chemistry and Environmental Science, Zhangzhou Normal College, Zhangzhou, PR China. zzsyliujiaming@163.com
authors.forename: Jiaming
authors.initials: J
authors.lastname: Liu
authors.forename: Liqing
authors.initials: L
authors.lastname: Zeng
authors.forename: Zhiming
authors.initials: Z
authors.lastname: Li
authors.forename: Fei
authors.initials: F
authors.lastname: Gao
authors.forename: Xiaomei
authors.initials: X
authors.lastname: Huang
authors.forename: Feiming
authors.initials: F
authors.lastname: Li
authors.forename: Huiqing
authors.initials: H
authors.lastname: Lin
mesh_terms: D000818:Animals; D002384:Catalysis; D002645:Chickens; D002851:Chromatography, High Pressure Liquid; D002976:Clenbuterol / Q000032:analysis* / Q000737:chemistry; D004372:Ducks; D004801:Eosine Yellowish-(YS) / Q000737:chemistry*; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D008163:Luminescent Measurements / Q000379:methods*; D010084:Oxidation-Reduction; D010504:Periodic Acid / Q000737:chemistry*; D012680:Sensitivity and Specificity; D013552:Swine; D013696:Temperature
chemical_list: D010504:Periodic Acid; C009288:metaperiodate; D004801:Eosine Yellowish-(YS); D002976:Clenbuterol