Effects of intermittent negative pressure on osteogenesis in human bone marrow-derived stroma cells.
OBJECTIVE
We investigated the effects of intermittent negative pressure on osteogenesis in human bone marrow-derived stroma cells (BMSCs) in vitro.


METHODS
BMSCs were isolated from adult marrow donated by a hip osteoarthritis patient with prosthetic replacement and cultured in vitro. The third passage cells were divided into negative pressure treatment group and control group. The treatment group was induced by negative pressure intermittently (pressure: 50 kPa, 30 min/times, and twice daily). The control was cultured in conventional condition. The osteogenesis of BMSCs was examined by phase-contrast microscopy, the determination of alkaline phosphatase (ALP) activities, and the immunohistochemistry of collagen type I. The mRNA expressions of osteoprotegerin (OPG) and osteoprotegerin ligand (OPGL) in BMSCs were analyzed by real-time polymerase chain reaction (PCR).


RESULTS
BMSCs showed a typical appearance of osteoblast after 2 weeks of induction by intermittent negative pressure, the activity of ALP increased significantly, and the expression of collagen type I was positive. In the treatment group, the mRNA expression of OPG increased significantly (P<0.05) and the mRNA expression of OPGL decreased significantly (P<0.05) after 2 weeks, compared with the control.


CONCLUSIONS
Intermittent negative pressure could promote osteogenesis in human BMSCs in vitro.
Yang
Zhi
Z
Department of Orthopaedics, the First Affiliated Hospital, School of Medicine, Xi'an Jiaotong University, Xi'an 710061, China.
Liu
Miao
M
Zhang
Yin-gang
YG
Guo
Xiong
X
Xu
Peng
P
D001854:Bone Marrow Cells / Q000166:cytology*; D002454:Cell Differentiation; D049109:Cell Proliferation; D002478:Cells, Cultured; D006801:Humans; D007150:Immunohistochemistry; D010012:Osteogenesis; D053244:Osteoprotegerin / Q000235:genetics; D011312:Pressure; D053245:RANK Ligand / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D017154:Stromal Cells / Q000166:cytology*
D053244:Osteoprotegerin; D053245:RANK Ligand; D012333:RNA, Messenger