title: Diallyl sulfides in garlic activate both TRPA1 and TRPV1.
abstract: We searched for novel agonists of TRP receptors especially for TRPA1 and TRPV1 in foods. We focused attention on garlic compounds, diallyl sulfide (DAS), diallyl disulfide (DADS), and diallyl trisulfide (DATS). In TRPA1 or TRPV1 heterogeneously expressed CHO cells, all of those compounds increased [Ca(2+)](i) in concentration-dependent manner. The EC(50) values of DADS and DATS were similar to that of allyl isothiocyanate (AITC) and that of DAS was 170-fold larger than that of AITC. Maximum responses of these sulfides were equal to that of AITC. The EC(50) values of these compounds for TRPV1 were around 100 microM against that of capsaicin (CAP), 25.6 nM and maximum responses of garlic compounds were half to that of CAP. The Ca(2+) responses were significantly suppressed by co-application of antagonist. We conclude that DAS, DADS, and DATS are agonist of both TRPA1 and TRPV1 but with high affinity for TRPA1.
authors.affiliation: Graduate School of Nutritional and Environmental Sciences, University of Shizuoka, 52-1 Yada, Suruga-ku, Shizuoka 422-8526, Japan.
authors.forename: Kanako
authors.initials: K
authors.lastname: Koizumi
authors.forename: Yusaku
authors.initials: Y
authors.lastname: Iwasaki
authors.forename: Masataka
authors.initials: M
authors.lastname: Narukawa
authors.forename: Yuji
authors.initials: Y
authors.lastname: Iitsuka
authors.forename: Tomomi
authors.initials: T
authors.lastname: Fukao
authors.forename: Taiichiro
authors.initials: T
authors.lastname: Seki
authors.forename: Toyohiko
authors.initials: T
authors.lastname: Ariga
authors.forename: Tatsuo
authors.initials: T
authors.lastname: Watanabe
mesh_terms: D000498:Allyl Compounds / Q000494:pharmacology*; D000818:Animals; D016466:CHO Cells; D015220:Calcium Channels / Q000235:genetics; D006224:Cricetinae; D003412:Cricetulus; D004220:Disulfides / Q000494:pharmacology*; D005737:Garlic / Q000737:chemistry*; D006801:Humans; D017953:Isocyanates / Q000494:pharmacology; D009419:Nerve Tissue Proteins / Q000819:agonists* / Q000235:genetics; D051381:Rats; D013440:Sulfides / Q000494:pharmacology*; D000074025:TRPA1 Cation Channel; D050916:TRPV Cation Channels / Q000819:agonists* / Q000235:genetics; D014162:Transfection; D050051:Transient Receptor Potential Channels / Q000819:agonists* / Q000235:genetics
chemical_list: D000498:Allyl Compounds; D015220:Calcium Channels; D004220:Disulfides; D017953:Isocyanates; D009419:Nerve Tissue Proteins; D013440:Sulfides; D000074025:TRPA1 Cation Channel; C473350:TRPA1 protein, human; D050916:TRPV Cation Channels; D050051:Transient Receptor Potential Channels; C493922:Trpv1 protein, rat; C491149:allyl isocyanate; C042577:diallyl trisulfide; C028009:diallyl disulfide; C038491:allyl sulfide