Flow cytometric immunophenotyping of peripheral T cell neoplasms using CD3 gating.
Peripheral T cell neoplasms (PTCNs) such as peripheral T cell lymphoma, adult T cell leukemia/lymphoma, and mycosis fungoides are associated with poorer prognoses compared to B cell neoplasms. Hence, an accurate and early diagnosis is necessary for the successful treatment of PTCNs; however, this can be difficult to achieve. In this study, flow cytometric immunophenotyping using CD3 gating was performed retrospectively on 56 samples from 52 patients diagnosed with PTCNs, and the analytical data were compared with the immunohistochemical findings. Abnormal CD3 T cell populations were distinguishable from the normal T cell population, using this CD3 gating strategy.
Hara
Satoshi
S
Department of Internal Medicine (I), Osaka Medical College, Osaka, Japan. in1211@poh.osaka-med.ac.jp
Yokote
Taiji
T
Akioka
Toshikazu
T
Miyoshi
Takuji
T
Ikemoto
Toshiyuki
T
Tanaka
Hidema
H
Takubo
Takayuki
T
Tsuji
Motomu
M
Hanafusa
Toshiaki
T
D017252:CD3 Complex; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods*; D016399:Lymphoma, T-Cell / Q000276:immunology / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D012189:Retrospective Studies; D013601:T-Lymphocytes / Q000276:immunology / Q000473:pathology*
D017252:CD3 Complex