A harmless method for determining trenbolone acetate together with 17beta-trenbolone in beef.
An inexpensive, harmless, and quick technique of sample preparation followed by a reversed-phase high-performance liquid chromatography (HPLC) for the simultaneous quantification of trenbolone acetate and 17beta-trenbolone in cattle muscle is presented. Analysis by HPLC with photo-diode array detector was performed using a short C1 column with an isocratic mobile phase. The method was validated by the analyses of spiked samples, resulting recoveries (> or = 87.9%; relative standard deviations < or = 3.4%), analytical total time (< 20 min/sample, where, a batch of 12 samples in 2 h), and quantitation limits (< or = 1.8 ng/g). No harmful organic solvents and reagents were used at all.
Furusawa
Naoto
N
Graduate School of Human Life Science, Osaka City University, Osaka 558-8585, Japan. furusawa@life.osaka-cu.ac.jp
D045930:Anabolic Agents / Q000032:analysis*; D000818:Animals; D002417:Cattle; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005506:Food Contamination / Q000032:analysis; D008460:Meat / Q000032:analysis*; D018482:Muscle, Skeletal / Q000737:chemistry; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D014204:Trenbolone Acetate / Q000031:analogs & derivatives* / Q000032:analysis
D045930:Anabolic Agents; D014204:Trenbolone Acetate