Transgenic lettuce seedlings carrying hepatitis B virus antigen HBsAg.
The obtainment of transgenic edible plants carrying recombinant antigens is a desired issue in search for economic alternatives viewing vaccine production. Here we report a strategy for genetic transformation of lettuce plants (Lactuca sativa L.) using the surface antigen HBsAg of hepatitis B virus. Transgenic lettuce seedlings were obtained through the application of a regulated balance of plant growth regulators. Genetic transformation process was acquired by cocultivation of cotyledons with Agrobacterium tumefaciens harboring the recombinant plasmid. It is the first description of a lettuce Brazilian variety 'Vitória de Verão' genetically modified.
Marcondes
Jackson
J
Biochemical Laboratory of Plants and Microorganisms, School of Agricultural Sciences and Veterinary, UNESP, Jaboticabal, SP, Brazil. jackson@fcav.unesp.br
Hansen
Ekkehard
E
D004274:DNA, Recombinant; D006514:Hepatitis B Surface Antigens / Q000235:genetics*; D017325:Hepatitis B Vaccines / Q000235:genetics*; D018545:Lactuca / Q000235:genetics* / Q000276:immunology; D010937:Plant Growth Regulators; D030821:Plants, Genetically Modified / Q000235:genetics* / Q000276:immunology; D036226:Seedlings / Q000235:genetics / Q000276:immunology; D022462:Vaccines, Edible
D004274:DNA, Recombinant; D006514:Hepatitis B Surface Antigens; D017325:Hepatitis B Vaccines; D010937:Plant Growth Regulators; D022462:Vaccines, Edible