Rapid and high-throughput purification of salvianolic acid B from Salvia miltiorrhiza Bunge by high-performance counter-current chromatography.
A large-scale purification of salvianolic acid B from Salvia miltiorrhiza Bunge is presented. The method development began with selection of the solvent system, then optimization of the operating parameters and ended up with linear scale-up from an analytical to a preparative instrument. Three factors were used for method optimization and scale-up estimation: purity, process throughput and process efficiency. Preparation was achieved using a two-phase solvent system comprising hexane-ethyl acetate-methanol-acetic acid-water (1:5:1.5:0.00596:5, v/v). This preparation yielded 475 mg of salvianolic acid B with a purity of 96.1% from 1.5 g of crude extract. The process throughput of crude was 2.23 g/h while process efficiency per gram of target compound was 0.769 g/h. Two factors-process environmental risk factor and process evaluation factor were used for evaluation of the separation process.
Zhang
Min
M
Modern Engineering Center for Traditional Chinese Medicine, School of Pharmacy, East China University of Science and Technology, Shanghai 200237, China.
Ignatova
Svetlana
S
Liang
Qionglin
Q
Wu Jun
Frank
F
Sutherland
Ian
I
Wang
Yiming
Y
Luo
Guoan
G
D001572:Benzofurans / Q000032:analysis / Q000302:isolation & purification*; D003377:Countercurrent Distribution / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000032:analysis / Q000302:isolation & purification*; D027561:Salvia miltiorrhiza / Q000737:chemistry*; D053630:Depsides
D001572:Benzofurans; D004365:Drugs, Chinese Herbal; C076944:salvianolic acid B; D053630:Depsides