title: Determination of streptomycin residues in honey by liquid chromatography-tandem mass spectrometry.
abstract: Streptomycin is an aminoglycoside antibiotic used in apiculture to protect bees against a variety of brood diseases. Brazilian authorities have included it in the National Regulatory Monitoring Program for honey production. A simple and reliable method using liquid chromatography-tandem mass spectrometry has been developed and validated for the determination of streptomycin in honey. The chromatography separation was performed on a Gemini 5 microm C18 (50 mm x 2 mm) column using 5mM heptafluorbutiric acid/acetonitrile (85:15) as the mobile phase at a flow rate of 0.2 mLmin(-1). The detection of the analyte was achieved by positive ionization electrospray in multiple reaction-monitoring modes. Two characteristic transitions were monitored for streptomycin. Some analytical parameters were validated according to the guidelines laid down by European Commission Decision 2002/657/EC: decision limit, detection capability, recovery, precision and ruggedness. The recoveries of streptomycin from honey fortified at 2.5, 10, 15 and 20 microgkg(-1) levels are around 100%. The decision limit and detection capability of streptomycin was 3 microgkg(-1) and 4.7 microgkg(-1) respectively.
authors.affiliation: Microbioticos Laboratories, Av. Santa Isabel 2120, Campinas, SP, 13084-471, Brazil.
authors.forename: Rodrigo H M M
authors.initials: RH
authors.lastname: Granja
authors.forename: Alfredo M Montes
authors.initials: AM
authors.lastname: Niño
authors.forename: Roberto A M
authors.initials: RA
authors.lastname: Zucchetti
authors.forename: Rosario E Montes
authors.initials: RE
authors.lastname: Niño
authors.forename: Raj
authors.initials: R
authors.lastname: Patel
authors.forename: Alessandro G
authors.initials: AG
authors.lastname: Salerno
mesh_terms: D000900:Anti-Bacterial Agents / Q000032:analysis* / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004350:Drug Residues / Q000032:analysis* / Q000302:isolation & purification; D006722:Honey / Q000032:analysis*; D015203:Reproducibility of Results; D013307:Streptomycin / Q000032:analysis* / Q000302:isolation & purification; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D000900:Anti-Bacterial Agents; D013307:Streptomycin