Highly efficient peptide separations in proteomics. Part 2: bi- and multidimensional liquid-based separation techniques.
Multidimensional liquid-based separation techniques are described for maximizing the resolution of the enormous number of peptides generated upon tryptic digestion of proteomes, and hence, reduce the spatial and temporal complexity of the sample to a level that allows successful mass spectrometric analysis. This review complements the previous contribution on unidimensional high performance liquid chromatography (HPLC). Both chromatography and electrophoresis will be discussed albeit with reversed-phase HPLC (RPLC) as the final separation dimension prior to MS analysis.
Sandra
Koen
K
Pronota nv, Technologiepark 4, VIB Bio-incubator, B-9052 Zwijnaarde/Ghent, Belgium. koen.sandra@richrom.com
Moshir
Mahan
M
D'hondt
Filip
F
Tuytten
Robin
R
Verleysen
Katleen
K
Kas
Koen
K
François
Isabelle
I
Sandra
Pat
P
D000818:Animals; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D019075:Electrophoresis, Capillary; D006801:Humans; D007525:Isoelectric Focusing; D010455:Peptides / Q000302:isolation & purification*; D040901:Proteomics / Q000379:methods*
D010455:Peptides