title: Identification and characterization of differentially expressed genes from Fagus sylvatica roots after infection with Phytophthora citricola.
abstract: Phytophthora species are major plant pathogens infecting herbaceous and woody plants including European beech, the dominant or co-dominant tree in temperate Europe and an economically important species. For the analysis of the interaction of Phytophthora citricola with Fagus sylvatica suppression subtractive hybridization was used to isolate transcripts induced during infection and 1,149 sequences were generated. Hybridizations with driver and tester populations demonstrated differential expression in infected roots as compared to controls and verify efficient enrichment of these cDNAs during subtraction. Up regulation of selected genes during pathogenesis demonstrated using RT-PCR is consistent with these results. Pathogenesis-related proteins formed the largest group among functionally categorized transcripts. Cell wall proteins and protein kinases were also frequently found. Several transcription factors were isolated that are reactive to pathogens or wounding in other plants. The library contained a number of jasmonic acid, salicylic acid and ethylene responsive genes as well as genes directly involved in signaling pathways. Besides a mechanistic interconnection among signaling pathways another factor explaining the activation of different pathways could be the hemibiotrophic life style of Phytophthora triggering different signals in both stages.
authors.affiliation: Forest Genetics, Center of Life and Food Sciences Weihenstephan, Technische Universität München, Freising, Germany. schlink@tum.de
authors.forename: Katja
authors.initials: K
authors.lastname: Schlink
mesh_terms: D020224:Expressed Sequence Tags; D029964:Fagus / Q000235:genetics / Q000378:metabolism* / Q000382:microbiology; D018506:Gene Expression Regulation, Plant; D015723:Gene Library; D018895:Microsatellite Repeats; D010838:Phytophthora / Q000472:pathogenicity*; D010935:Plant Diseases / Q000235:genetics*; D018517:Plant Roots / Q000235:genetics / Q000378:metabolism* / Q000382:microbiology; D018749:RNA, Plant / Q000378:metabolism; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D015854:Up-Regulation
chemical_list: D018749:RNA, Plant