title: Hippocampal long-term potentiation is enhanced in urethane-anesthetized RGS2 knockout mice.
abstract: RGS2 is a member of the regulator of G-protein signaling (RGS) family and has been implicated in cellular mechanisms associated with neuronal plasticity. Long-term potentiation (LTP) of RGS2 knockout and wild-type mice was examined at the Schaffer collaterals to CA1 pathway in urethane-anesthetized mice in vivo to examine RGS2's possible role in the regulation of potentiation. As compared to wild-type mice, RGS2 knockouts demonstrated much stronger LTP of the extracellular population spikes at the somatic and dendritic layers in CA1 region and more pronounced LTP of the population excitatory postsynaptic current sink. Under baseline conditions, RGS2 knockouts showed lower paired-pulse facilitation of the excitatory postsynaptic potentials and associated current sinks in vivo as compared with wild-type mice. The data show for the first time that RGS2 deficient mice in vivo differ from wild-type mice in both short-term and long-term synaptic plasticity suggesting that RGS2 serves as a negative regulator of long-term synaptic plasticity.
authors.affiliation: Graduate Program in Neuroscience, Department of Physiology and Pharmacology, University of Western Ontario, London, Ontario, Canada.
authors.forename: R Matthew
authors.initials: RM
authors.lastname: Hutchison
authors.forename: Peter
authors.initials: P
authors.lastname: Chidiac
authors.forename: L Stan
authors.initials: LS
authors.lastname: Leung
mesh_terms: D000200:Action Potentials; D000704:Analysis of Variance; D018686:Anesthetics, Intravenous; D000818:Animals; D004558:Electric Stimulation; D019706:Excitatory Postsynaptic Potentials; D006624:Hippocampus / Q000187:drug effects / Q000502:physiology*; D017774:Long-Term Potentiation / Q000187:drug effects / Q000502:physiology*; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D008839:Microelectrodes; D009474:Neurons / Q000187:drug effects / Q000502:physiology*; D020710:RGS Proteins / Q000235:genetics / Q000378:metabolism*; D055366:Synaptic Potentials; D013997:Time Factors; D014520:Urethane
chemical_list: D018686:Anesthetics, Intravenous; D020710:RGS Proteins; C105345:Rgs2 protein, mouse; D014520:Urethane