title: Neuroactive flavonoid glycosides from Tilia petiolaris DC. extracts.
abstract: Pharmacological assay guided purification of an ethanol extract of Tilia petiolaris DC. inflorescences resulted in the isolation and identification of isoquercitrin (ISO), quercetin 3-O-glucoside-7-O-rhamnoside (QUE) and kaempferol 3-O-glucoside-7-O-rhamnoside (KAE). The behavioral actions of these glycosylated flavonoids were examined in the hole board, locomotor activity and thiopental-induced loss of righting reflex tests in mice. QUE (10 and 30 mg/kg) and KAE (30 mg/kg), intraperitoneally (i.p.) administered to mice, reduced all the parameters measured in the hole board test, but ISO (30 mg/kg) only reduced the number of rearings. Meanwhile QUE at 30 mg/kg i.p. also decreased the ambulatory locomotor activity and increased the sodium thiopental-induced time of loss of the righting reflex suggesting a clear depressant action. The above results demonstrate the occurrence of neuroactive flavonoid glycosides in Tilia.
authors.affiliation: Instituto de Química y Fisicoquímica Biológicas, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Buenos Aires, Argentina.
authors.forename: Leonardo M
authors.initials: LM
authors.lastname: Loscalzo
authors.forename: Cristina
authors.initials: C
authors.lastname: Wasowski
authors.forename: Mariel
authors.initials: M
authors.lastname: Marder
mesh_terms: D000818:Animals; D001522:Behavior, Animal / Q000187:drug effects*; D005419:Flavonoids / Q000302:isolation & purification / Q000494:pharmacology; D005960:Glucosides / Q000302:isolation & purification / Q000494:pharmacology*; D006031:Glycosylation; D006993:Hypnotics and Sedatives / Q000302:isolation & purification / Q000494:pharmacology*; D056626:Inflorescence; D044949:Kaempferols / Q000302:isolation & purification / Q000494:pharmacology*; D008297:Male; D051379:Mice; D009043:Motor Activity / Q000187:drug effects*; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D011597:Psychomotor Performance / Q000187:drug effects*; D011794:Quercetin / Q000031:analogs & derivatives* / Q000302:isolation & purification / Q000494:pharmacology; D032384:Tilia / Q000737:chemistry*
chemical_list: D005419:Flavonoids; D005960:Glucosides; D006993:Hypnotics and Sedatives; D044949:Kaempferols; D010936:Plant Extracts; C546610:kaempferol 3-O-glucoside-7-O-rhamnoside; C546609:quercetin 3-O-glucoside-7-O-rhamnoside; C016527:isoquercitrin; D011794:Quercetin