Tyrosine kinase B receptor and BDNF expression in ovarian cancers - Effect on cell migration, angiogenesis and clinical outcome.
In this report, we demonstrated that overexpression of tropomyosin-related kinase B (TrkB) was associated with shorter survival in ovarian cancer patients. Brain-derived neurotrophic factor (BDNF), the TrkB ligand, induced activation (phosphorylation) of TrkB in a dose dependent manner. Besides demonstrating the effect of BDNF/TrkB pathway in enhancing cancer cell migration and invasion but inhibiting apoptosis, we also report for the first time that exogenous hepatocyte growth factor induced TrkB expression at both mRNA and protein levels as well as phosphorylation. Our findings suggest that BDNF/TrkB pathway is important in ovarian carcinogenesis and TrkB may be a potential therapeutic target for ovarian cancer.
Au
Christy W H
CW
Department of Pathology, The University of Hong Kong, Queen Mary Hospital, Hong Kong.
Siu
Michelle K Y
MK
Liao
Xiaoyun
X
Wong
Esther S Y
ES
Ngan
Hextan Y S
HY
Tam
Kar Fai
KF
Chan
Dominic C W
DC
Chan
Queeny K Y
QK
Cheung
Annie N Y
AN
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015153:Blotting, Western; D019208:Brain-Derived Neurotrophic Factor / Q000096:biosynthesis*; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000502:physiology*; D005260:Female; D020868:Gene Silencing; D017228:Hepatocyte Growth Factor / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D047468:Immunoprecipitation; D020287:In Situ Nick-End Labeling; D053208:Kaplan-Meier Estimate; D008875:Middle Aged; D009361:Neoplasm Invasiveness / Q000235:genetics / Q000473:pathology; D009389:Neovascularization, Pathologic / Q000235:genetics / Q000378:metabolism*; D010051:Ovarian Neoplasms / Q000378:metabolism* / Q000401:mortality / Q000473:pathology*; D011379:Prognosis; D012333:RNA, Messenger / Q000032:analysis; D034741:RNA, Small Interfering; D020813:Receptor, trkB / Q000096:biosynthesis*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D040301:Vascular Endothelial Growth Factor Receptor-2 / Q000378:metabolism; D055815:Young Adult
D019208:Brain-Derived Neurotrophic Factor; D012333:RNA, Messenger; D034741:RNA, Small Interfering; D017228:Hepatocyte Growth Factor; D020813:Receptor, trkB; D040301:Vascular Endothelial Growth Factor Receptor-2