Genotyping of Brucella melitensis by rpoB gene analysis and re-evaluation of conventional serotyping method.
In Turkey, where brucellosis is endemic, a comparison of conventional and molecular genotyping methods has not been published to date. In this study, we investigated the efficacy of single nucleotide polymorphism (SNP) analysis of rpoB gene in the genotyping of Brucella melitensis strains by sequencing. In light of the molecular genotyping method available now in Turkey, the adequacy of serological typing alone should be re-evaluated as a tool for epidemiologic studies of B. melitensis.
Sayan
Murat
M
Clinical Laboratory, PCR Unit, Faculty of Medicine, University of Kocaeli, Kocaeli, Turkey. sayanmurat@hotmail.com
Yumuk
Zeki
Z
Bilenoglu
Onur
O
Erdenlig
Sevil
S
Willke
Ayse
A
D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics*; D017347:Brucella melitensis / Q000145:classification* / Q000235:genetics*; D002006:Brucellosis / Q000382:microbiology* / Q000662:veterinary*; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D012321:DNA-Directed RNA Polymerases / Q000235:genetics*; D005838:Genotype; D006041:Goats / Q000382:microbiology; D006801:Humans; D017720:Molecular Epidemiology / Q000379:methods; D020641:Polymorphism, Single Nucleotide; D017422:Sequence Analysis, DNA; D012703:Serotyping; D012756:Sheep / Q000382:microbiology; D014421:Turkey
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D012321:DNA-Directed RNA Polymerases; C105078:RNA polymerase beta subunit