title: Ameliorative effect of 1,2-benzenedicarboxylic acid dinonyl ester against amyloid beta peptide-induced neurotoxicity.
abstract: Amyloid beta peptide (Abeta)-induced oxidative stress may be linked to neurodegenerative disease. Ethanol extracts of Rosa laevigata protected PC12 cells from hydrogen peroxide-induced oxidative stress. (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) reduction assays revealed a significant increase in cell viability when oxidatively stressed PC12 cells were treated with R. laevigata extract. The effect of R. laevigata on oxidative stress-induced cell death was further investigated by lactate dehydrogenase release assays and trypan blue exclusion assays. Administration of 1,2-benzenedicarboxylic acid dinonyl ester from R. laevigata extract to mice infused with Abeta significantly reversed learning and memory impairment in behavioural tests. After behavioural testing, the mice were sacrificed and brains were collected for the examination of lipid peroxidation, catalase activity and acetylcholinesterase (AchE) activity. These results suggest that 1,2-benzenedicarboxylic acid dinonyl ester from R. laevigata extract may be able to reduce Abeta-induced neurotoxicity, possibly by reducing oxidative stress. Therefore, R. laevigata extract may be useful for the prevention of oxidative stress-induced neurodegenerative disorders.
authors.affiliation: Department of Food Technology, Korea University, Seoul, Korea.
authors.forename: Soo
authors.initials: S
authors.lastname: Jung Choi
authors.forename: Mi Jeong
authors.initials: MJ
authors.lastname: Kim
authors.forename: Ho
authors.initials: H
authors.lastname: Jin Heo
authors.forename: Jae Kyeum
authors.initials: JK
authors.lastname: Kim
authors.forename: Woo
authors.initials: W
authors.lastname: Jin Jun
authors.forename: Hye Kyung
authors.initials: HK
authors.lastname: Kim
authors.forename: Eun-Ki
authors.initials: EK
authors.lastname: Kim
authors.forename: Myeong
authors.initials: M
authors.lastname: Ok Kim
authors.forename: Hong
authors.initials: H
authors.lastname: Yon Cho
authors.forename: Han-Joon
authors.initials: HJ
authors.lastname: Hwang
authors.forename: Young
authors.initials: Y
authors.lastname: Jun Kim
authors.forename: Dong-Hoon
authors.initials: DH
authors.lastname: Shin
mesh_terms: D016564:Amyloid beta-Protein Precursor / Q000633:toxicity*; D000818:Animals; D001362:Avoidance Learning / Q000187:drug effects; D016923:Cell Death / Q000187:drug effects; D002462:Cell Membrane / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002800:Cholinesterase Inhibitors / Q000494:pharmacology; D006861:Hydrogen Peroxide / Q000633:toxicity; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D018782:Maze Learning / Q000187:drug effects; D051379:Mice; D008813:Mice, Inbred ICR; D020258:Neurotoxicity Syndromes / Q000378:metabolism / Q000517:prevention & control*; D018384:Oxidative Stress / Q000187:drug effects; D016716:PC12 Cells; D010795:Phthalic Acids / Q000494:pharmacology / Q000627:therapeutic use*; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology; D051381:Rats; D027826:Rosa / Q000737:chemistry
chemical_list: D016564:Amyloid beta-Protein Precursor; D002800:Cholinesterase Inhibitors; D010795:Phthalic Acids; D010936:Plant Extracts; C019174:dinonylphthalate; D006861:Hydrogen Peroxide