Methyl jasmonate induced accumulation of kalopanaxsaponin I in Nigella sativa.
Hydroponically cultivated Nigella sativa L. plants treated with methyl jasmonate (MeJA) showed a twelve-fold increase in levels of the monodesmosidic triterpene saponins alpha-hederin and kalopanaxsaponin I (KsI) in the leaves. We will demonstrate that these two saponins accounted for approximately 10% of the dry plant matter, of which 93% was KsI and 7% alpha-hederin. To address the molecular basis of saponin induction by MeJA, we cloned and characterized the beta-amyrin synthase gene (NsbetaAS1) encoding one of the key enzymes in triterpene saponin biosynthesis. As expected, NsbetaAS1 transcription was induced by MeJA and led to the production of beta-amyrin when over-expressed in yeast.
Scholz
Martin
M
Westphalian Wilhelms-University Münster, Institute of Biochemistry and Biotechnology of Plants, Hindenburgplatz 55, 48143 Münster, Germany.
Lipinski
Marta
M
Leupold
Marco
M
Luftmann
Heinrich
H
Harig
Lena
L
Ofir
Rivka
R
Fischer
Rainer
R
Prüfer
Dirk
D
Müller
Kai J
KJ
D000085:Acetates / Q000494:pharmacology*; D002851:Chromatography, High Pressure Liquid; D003517:Cyclopentanes / Q000494:pharmacology*; D019751:Intramolecular Transferases / Q000737:chemistry / Q000235:genetics* / Q000502:physiology; D031881:Nigella sativa / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D009828:Oleanolic Acid / Q000031:analogs & derivatives* / Q000096:biosynthesis / Q000737:chemistry / Q000302:isolation & purification; D054883:Oxylipins / Q000494:pharmacology*; D010937:Plant Growth Regulators / Q000494:pharmacology*; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics* / Q000502:physiology; D012503:Saponins / Q000096:biosynthesis* / Q000737:chemistry / Q000302:isolation & purification; D014158:Transcription, Genetic / Q000187:drug effects; D015003:Yeasts / Q000235:genetics
D000085:Acetates; D003517:Cyclopentanes; D054883:Oxylipins; D010937:Plant Growth Regulators; D010940:Plant Proteins; D012503:Saponins; C010177:beta-hederin; C117027:kalopanax saponin I; D009828:Oleanolic Acid; C072239:methyl jasmonate; D019751:Intramolecular Transferases; C039924:2,3-oxidosqualene-beta-amyrin-cyclase