Blood outgrowth endothelial cell migration and trapping in vivo: a window into gene therapy.
Human blood outgrowth endothelial cells (hBOECs) may be useful delivery cells for gene therapy. hBOECs have high expansion capacity and a stable phenotype. If incorporated into blood vessels, hBOECs could release therapeutic agents directly into the bloodstream. However, little is known about the lodging and homing of hBOECs in vivo. We examined the homing patterns of hBOECs in mice and explored extending cell-based factor VIII (FVIII) gene therapy from mice to larger animals. hBOECs were injected into NOD/SCID mice to determine where they localize, how localization changes over time, and if there were toxic effects on host organs. The presence of hBOECs in mouse organs was determined by quantitative polymerase chain reaction (qPCR) and immunofluorescence microscopy. hBOECs lodged most notably in mouse lungs at 3 h, but by 24 h, no differences were observed among 9 organs. The longevity of hBOECs was assessed up to 7 months in vivo. hBOECs expanded well and then reached a plateau in vivo. hBOECs from older cultures expanded equally well in vivo as younger hBOECs. hBOECs caused no noticeable organ toxicity up to 3 days after injection. When mice were pretreated with antibodies to E-selectin, P-selectin, or anti-alpha4 integrin prior to hBOEC injection, the number of hBOECs in lungs at 3 h was decreased. Preliminary studies that infused hemophilic dogs with autologous canine BOECs that overexpressed FVIII (B-domain deleted) showed improvement in whole blood clotting times (WBCTs). In conclusion, the survivability, expandability, and lack of toxicity of BOECs in vivo indicate that they may be valuable host cells for gene therapy.
Milbauer
Liming C
LC
Department of Medicine, University of Minnesota Medical School, Minneapolis, Minn 55455, USA.
Enenstein
Judy A
JA
Roney
Mark
M
Solovey
Anna
A
Bodempudi
Vidya
V
Nichols
Timothy C
TC
Hebbel
Robert P
RP
D000818:Animals; D001483:Base Sequence; D017931:DNA Primers; D004285:Dogs; D004730:Endothelium, Vascular / Q000166:cytology* / Q000378:metabolism; D005169:Factor VIII / Q000235:genetics*; D015316:Genetic Therapy; D051379:Mice; D016688:Mice, Inbred NOD; D016513:Mice, SCID; D008856:Microscopy, Fluorescence; D016133:Polymerase Chain Reaction
D017931:DNA Primers; D005169:Factor VIII