title: Determination of lumefantrine in rat plasma by liquid-liquid extraction using LC-MS/MS with electrospray ionization: assay development, validation and application to a pharmacokinetic study.
abstract: A simple, sensitive and rapid method for the analysis of lumefantrine in rat plasma using liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) was developed. Detection was performed by positive ion electrospray ionization (ESI) in multiple reaction monitoring (MRM) mode. The method included a chromatographic run of 5 min using a C(18) analytical column and the calibration curve was linear over the concentration range of 2-500 ng/mL with a correlation coefficient (r) of 0.996 or better. The intra- and inter-day assay precision ranged from 1.5 to 7.5% and 5.5 to 7.7%, respectively, and intra- and inter-day assay accuracy was between 91.3-109.7% and 97.0-104.7%, respectively. The method was successfully applied for the pharmacokinetic study in rats.
authors.affiliation: Pharmacokinetics and Metabolism Division, Central Drug Research Institute, Lucknow 226001, Uttar Pradesh, India.
authors.lastname: Wahajuddin
authors.forename: Sheelendra Pratap
authors.initials: SP
authors.lastname: Singh
authors.forename: Girish Kumar
authors.initials: GK
authors.lastname: Jain
mesh_terms: D000818:Animals; D000962:Antimalarials / Q000097:blood* / Q000493:pharmacokinetics; D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D004983:Ethanolamines / Q000097:blood* / Q000493:pharmacokinetics; D005449:Fluorenes / Q000097:blood* / Q000493:pharmacokinetics; D007202:Indicators and Reagents; D000078102:Lumefantrine; D008297:Male; D010616:Phenanthrenes / Q000097:blood / Q000493:pharmacokinetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D012015:Reference Standards; D015203:Reproducibility of Results; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D000962:Antimalarials; D004983:Ethanolamines; D005449:Fluorenes; D007202:Indicators and Reagents; D010616:Phenanthrenes; D000078102:Lumefantrine; C023768:halofantrine