IGF-1 and G-CSF complement each other in BMSC migration towards infarcted myocardium in a novel in vitro model.
Stem cell capability enhanced with cytokine administration is a promising treatment for myocardial infarction. Bone marrow stem cells (BMSCs) were isolated from C57BL/6 mice (8-12 weeks old) expressing GFP and characterized with c-kit and CD34. Infarcted heart tissue fragments were placed into dishes with BMSCs and medium supplemented with G-CSF, SCF, IGF-1 or combinations thereof were given to the BMSC-infarcted myocardium in vitro model. The IGF-1-G-CSF group showed significantly higher migration (67.7% +/- 2.6) of c-kit(+) BMSCs towards the ischemic tissue and expressed MEF-2 (43.7% +/- 1.7). Of the single treatment groups, the G-CSF group demonstrated significantly higher migration of c-kit(+) BMSCs (60.5 +/- 2.7) with MEF-2 expression (38.7 +/- 1.4). IGF-1 complements G-CSF and was relatively more significant in its effects on BMSC migration and cardiac lineage commitment towards ischemic heart tissue.
Khan
Mohsin
M
National Center of Excellence in Molecular Biology (CEMB), University of the Punjab, Lahore, Pakistan.
Manzoor
Sobia
S
Mohsin
Sadia
S
Khan
Shaheen N
SN
Ahmad
Fridoon Jawad
FJ
D000818:Animals; D018952:Antigens, CD34 / Q000378:metabolism; D001854:Bone Marrow Cells / Q000502:physiology*; D002465:Cell Movement / Q000187:drug effects*; D016179:Granulocyte Colony-Stimulating Factor / Q000494:pharmacology*; D007334:Insulin-Like Growth Factor I / Q000494:pharmacology*; D051379:Mice; D008810:Mice, Inbred C57BL; D009203:Myocardial Infarction / Q000473:pathology / Q000503:physiopathology*; D009206:Myocardium / Q000473:pathology; D018006:Myogenic Regulatory Factors / Q000378:metabolism; D019009:Proto-Oncogene Proteins c-kit / Q000378:metabolism; D013234:Stem Cells / Q000378:metabolism / Q000502:physiology*
D018952:Antigens, CD34; D018006:Myogenic Regulatory Factors; D016179:Granulocyte Colony-Stimulating Factor; D007334:Insulin-Like Growth Factor I; D019009:Proto-Oncogene Proteins c-kit