title: Indolylarylsulfones bearing natural and unnatural amino acids. Discovery of potent inhibitors of HIV-1 non-nucleoside wild type and resistant mutant strains reverse transcriptase and coxsackie B4 virus.
abstract: New potent indolylarylsulfone (IAS) HIV-1 NNRTIs were obtained by coupling natural and unnatural amino acids to the 2-carboxamide and introducing different electron-withdrawing substituents at position 4 and 5 of the indole nucleus. The new IASs inhibited the HIV-1 replication in human T-lymphocyte (CEM) cells at low/subnanomolar concentration and were weakly cytostatic. Against the mutant L100I, K103N, and Y181C RT HIV-1 strains in CEM cells, sulfones 3, 4, 19, 27, and 31 were comparable to EFV. The new IASs were inhibitors to Coxsackie B4 virus at low micromolar (2-9 microM) concentrations. Superimposition of PLANTS docked conformations of IASs 19 and 9 revealed different hydrophobic interactions of the 3,5-dimethylphenyl group, for which a staking interaction with Tyr181 aromatic side chain was observed. The binding mode of 19 was not affected by the L100I mutation and was consistent with the interactions reported for the WT strain.
authors.affiliation: Dipartimento di Chimica e Tecnologie del Farmaco, Istituto Pasteur-Fondazione Cenci Bolognetti, Sapienza Universita di Roma, Roma, Italy.
authors.forename: Francesco
authors.initials: F
authors.lastname: Piscitelli
authors.forename: Antonio
authors.initials: A
authors.lastname: Coluccia
authors.forename: Andrea
authors.initials: A
authors.lastname: Brancale
authors.forename: Giuseppe
authors.initials: G
authors.lastname: La Regina
authors.forename: Anna
authors.initials: A
authors.lastname: Sansone
authors.forename: Cesare
authors.initials: C
authors.lastname: Giordano
authors.forename: Jan
authors.initials: J
authors.lastname: Balzarini
authors.forename: Giovanni
authors.initials: G
authors.lastname: Maga
authors.forename: Samantha
authors.initials: S
authors.lastname: Zanoli
authors.forename: Alberta
authors.initials: A
authors.lastname: Samuele
authors.forename: Roberto
authors.initials: R
authors.lastname: Cirilli
authors.forename: Francesco
authors.initials: F
authors.lastname: La Torre
authors.forename: Antonio
authors.initials: A
authors.lastname: Lavecchia
authors.forename: Ettore
authors.initials: E
authors.lastname: Novellino
authors.forename: Romano
authors.initials: R
authors.lastname: Silvestri
mesh_terms: D000596:Amino Acids / Q000737:chemistry*; D000818:Animals; D019380:Anti-HIV Agents / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology; D000970:Antineoplastic Agents / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology; D000998:Antiviral Agents / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D054697:Cytostatic Agents / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology; D024882:Drug Resistance, Viral; D029822:Enterovirus B, Human / Q000187:drug effects*; D054303:HIV Reverse Transcriptase / Q000378:metabolism; D015497:HIV-1 / Q000187:drug effects* / Q000201:enzymology / Q000235:genetics; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D007211:Indoles / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology; D008214:Lymphocytes / Q000187:drug effects / Q000821:virology; D051379:Mice; D008958:Models, Molecular; D008968:Molecular Conformation; D009154:Mutation; D011485:Protein Binding; D018894:Reverse Transcriptase Inhibitors / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology; D013237:Stereoisomerism; D013329:Structure-Activity Relationship; D013450:Sulfones / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology; D014779:Virus Replication
chemical_list: D000596:Amino Acids; D019380:Anti-HIV Agents; D000970:Antineoplastic Agents; D000998:Antiviral Agents; D054697:Cytostatic Agents; D007211:Indoles; D018894:Reverse Transcriptase Inhibitors; D013450:Sulfones; D054303:HIV Reverse Transcriptase