title: Congenital candidiasis: confirmation of mother-neonate transmission using molecular analysis techniques.
abstract: We describe a case of congenital acquired candidiasis in a preterm female delivered through Caesarean section due to the premature rupture of the amniotic membrane. The neonate presented with suspected chorioamnionitis and erythematous desquamative skin. Candida albicans was isolated from the placenta, mouth, groin, and periumbilical lesions. The infant developed candidemia due to Candida albicans and the same yeast was also isolated from a catheter. Culture inoculated with swabs from the mouth and vagina of the mother yielded C. albicans and C. krusei. All C. albicans isolates from the mother and the neonate were visually indistinguishable by molecular typing techniques which included chromosomal karyotyping and restriction endonuclease analysis followed by pulsed-field gel electrophoresis. These findings allowed the clinical condition to be confirmed as congenital acquisition of candidiasis in this case.
authors.affiliation: Infectious Diseases Division, Hospital de Clínicas José de San Martín University of Buenos Aires, Buenos Aires, Argentina. micologia@hospitaldeclinicas.uba.ar
authors.forename: Iris Carla Niveyro
authors.initials: IC
authors.lastname: Tiraboschi
authors.forename: Carla
authors.initials: C
authors.lastname: Niveyro
authors.forename: Ana Maria
authors.initials: AM
authors.lastname: Mandarano
authors.forename: Shawn A
authors.initials: SA
authors.lastname: Messer
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Bogdanowicz
authors.forename: Isabel
authors.initials: I
authors.lastname: Kurlat
authors.forename: María Beatriz
authors.initials: MB
authors.lastname: Lasala
mesh_terms: D002176:Candida albicans / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D002177:Candidiasis / Q000175:diagnosis* / Q000635:transmission*; D002404:Catheterization; D016172:DNA Fingerprinting; D004783:Environmental Microbiology; D005260:Female; D016469:Fungemia / Q000382:microbiology; D005838:Genotype; D006119:Groin / Q000382:microbiology; D006801:Humans; D007231:Infant, Newborn; D018445:Infectious Disease Transmission, Vertical; D007621:Karyotyping; D009035:Mothers; D009055:Mouth / Q000382:microbiology; D016533:Mycological Typing Techniques; D010920:Placenta / Q000382:microbiology; D012150:Polymorphism, Restriction Fragment Length; D011247:Pregnancy; D011251:Pregnancy Complications, Infectious / Q000382:microbiology*; D047928:Premature Birth; D014472:Umbilicus / Q000382:microbiology