title: Nanocomposite based on modified TiO2-BSA for functional applications.
abstract: A novel nanocomposite based on TiO(2)-protein for functional applications was prepared and characterized. The composite was made by covalent immobilisation of bovine serum albumin (BSA) on to the TiO(2) particles. BSA was attached to the TiO(2) powder through a three-step process. This involves the functionalization of the TiO(2) particles with silane and the subsequent coupling by glutaraldehyde to free NH(2) groups of the protein. Formation of Schiff's base was confirmed by IR spectroscopy and the optimum loading of the BSA was found to be 73.61%. Surface morphology of the composite was studied by SEM and TEM. Thermal analysis of TiO(2)-BSA composite was carried out by TGA and DSC. Structural variation of the BSA after immobilisation was studied by CD spectra.
authors.affiliation: Chemical Sciences and Technology Division, National Institute for Interdisciplinary Science and Technology (CSIR), Thiruvananthapuram, India.
authors.forename: C K
authors.initials: CK
authors.lastname: Simi
authors.forename: T Emilia
authors.initials: TE
authors.lastname: Abraham
mesh_terms: D000818:Animals; D002152:Calorimetry, Differential Scanning; D002417:Cattle; D002942:Circular Dichroism; D055767:Immobilized Proteins; D053761:Nanocomposites / Q000737:chemistry* / Q000648:ultrastructure; D012545:Schiff Bases / Q000737:chemistry; D012710:Serum Albumin, Bovine / Q000378:metabolism*; D012821:Silanes / Q000737:chemistry; D013818:Thermogravimetry; D014025:Titanium / Q000737:chemistry*; D014961:X-Ray Diffraction
chemical_list: D055767:Immobilized Proteins; D012545:Schiff Bases; D012821:Silanes; C009495:titanium dioxide; D012710:Serum Albumin, Bovine; D014025:Titanium