A membrane interferometer.
Freestanding phospholipid bilayers have been assembled spanning shallow, micrometer-sized wells etched into a Si wafer substrate so that the bilayers are near (within hundreds of nanometers) but not in contact with the wafer surface. The proximity of the bilayers to the highly reflective Si/SiO(2) interface allows them to be probed by using fluorescence-interference techniques. These interferometry measurements show that the bilayers are curved and that the curvature can be varied by changes in osmotic pressure. Furthermore, the ionophore gramicidin can be incorporated into the bilayers, making them selectively permeable to monovalent cations. This freestanding architecture may overcome surface-interaction problems that occur when cell membrane proteins are introduced into solid supported bilayers, while also allowing for high-precision measurements of changes in fluorophore position by interferometry.
Ganesan
Prasad V
PV
Department of Chemistry, Stanford University, Stanford, CA 94305-5080, USA.
Boxer
Steven G
SG
D002462:Cell Membrane / Q000648:ultrastructure*; D005453:Fluorescence; D006096:Gramicidin; D007368:Interferometry / Q000295:instrumentation / Q000379:methods*; D008051:Lipid Bilayers; D008722:Methods; D009997:Osmotic Pressure; D012825:Silicon; D012822:Silicon Dioxide
D008051:Lipid Bilayers; D006096:Gramicidin; D012822:Silicon Dioxide; D012825:Silicon