Measurement of phosphorylated extracellular signal-regulated kinase 1 and 2 in an undergraduate teaching laboratory with ALPHAscreen technology.
Teaching the practical aspects of signal transduction to large undergraduate classes can be challenging when there is only a finite time frame in which to engage in laboratory activities. This teaching resource describes the use of bead-based ALPHAscreen technology for a class of 300 second-year biochemistry students, exposing the next generation of researchers to cutting-edge technology. Although in this case phosphorylated extracellular signal-regulated kinase 1 and 2 were measured, this technology is applicable to the measurement of many different signaling components. This resource provides a practical guide for instructors and exemplifies how such traditionally high-throughput research technologies can be used as teaching tools.
Hay
Debbie L
DL
University of Auckland, New Zealand. dl.hay@auckland.ac.nz
D001671:Biochemistry / Q000193:education*; D002116:Calcitonin; D019411:Clinical Laboratory Techniques; D004504:Education, Medical, Undergraduate / Q000379:methods*; D008863:Microspheres; D019950:Mitogen-Activated Protein Kinase 1 / Q000032:analysis* / Q000378:metabolism; D048052:Mitogen-Activated Protein Kinase 3 / Q000032:analysis* / Q000378:metabolism; D010766:Phosphorylation; D015398:Signal Transduction / Q000502:physiology*; D013664:Teaching Materials
D002116:Calcitonin; D019950:Mitogen-Activated Protein Kinase 1; D048052:Mitogen-Activated Protein Kinase 3