[Study on virulence factors associated with biofilm formation and phylogenetic groupings in Escherichia coli strains isolated from patients with cystitis].
Escherichia coli samples isolated from female patients with cystitis were characterized with regard to the presence of virulence factors associated with biofilm formation and phylogenetic groupings. Polymerase chain reaction results demonstrated that all the samples were positive for the gene fimH (type 1 fimbriae), 91 for fliC (flagellins), 50 for papC (P fimbriae), 44 for kpsMTII (capsules) and 36 for flu (antigen 43). The results from assays to quantify the biofilm formation demonstrated that 44 samples produced biofilm on polystyrene microplates and 56 samples produced weak or no biofilm. We also confirmed that Escherichia coli samples were present in phylogenetic groups B2 and D.
Tiba
Monique Ribeiro
MR
Laboratório de Antígenos Bacterianos II, Departamento de Microbiologia e Imunologia, Instituto de Biologia, Universidade Estadual de Campinas, SP.
Nogueira
Gustavo Prado
GP
Leite
Domingos da Silva
Dda S
D018441:Biofilms / Q000254:growth & development*; D003556:Cystitis / Q000382:microbiology*; D004926:Escherichia coli / Q000235:genetics / Q000472:pathogenicity*; D004927:Escherichia coli Infections / Q000382:microbiology*; D005260:Female; D005838:Genotype; D006801:Humans; D010802:Phylogeny; D037521:Virulence Factors
D037521:Virulence Factors