Effects of metoclopramide-induced hyperprolactinemia on the prolactin receptor of murine endometrium.
OBJECTIVE
To evaluate the effects of metoclopramide-induced hyperprolactinemia on the prolactin receptor of murine endometrium.


METHODS
Experimental study using the RNA extraction to detect tissue prolactin receptor isoforms by reverse-transcriptase polymerase chain reaction (RT-PCR).


METHODS
University-based laboratory.


METHODS
Seventy-two female swiss albino mice (Mus musculus), approximately 100 days old, were divided into six 12-animal groups: (GI) nonoophorectomized mice given vehicle; (GII) nonoophorectomized mice treated with metoclopramide; (GIII) oophorectomized mice treated with metoclopramide; (GIV) oophorectomized mice treated with metoclopramide and 17beta-estradiol; (GV) oophorectomized mice treated with metoclopramide and micronized progesterone; (GVI) oophorectomized mice treated with metoclopramide and a solution of 17beta-estradiol and micronized progesterone.


METHODS
Drugs were administered for 50 days. Following euthanasia, the middle portions of the uterine horns were removed, sectioned, and immediately frozen for RT-PCR procedures. Blood was collected for the dosage of prolactin and serum estrogen and progesterone using radioimmune assay.


METHODS
Identification of uterine prolactin receptor isoforms.


RESULTS
The PRL receptor and its isoform L were identified only in GI (control group) and GII (metoclopramide), the two groups with nonoophorectomized animals. The amount of PRL receptor mRNA and that of its isoform L from GII were the largest. No other isoforms of the prolactin receptor were identified in any of the groups.


CONCLUSIONS
Our results suggest that replacement of estrogen and progestin may not increase the mRNA of endometrial PRL receptor in metoclopromide-induced hyperprolactinemia in rats after castration.
Rossi
Alexandre Guilherme Zabeu
AG
Gynecology Department, Federal University of São Paulo, Brazil.
Teixeira Gomes
Regina Célia
RC
de Jesus Simões
Manuel
M
Dos Santos Simões
Ricardo
R
Oliveira
Patrícia B
PB
Soares
José Maria
JM
Baracat
Edmund C
EC
D000818:Animals; D004195:Disease Models, Animal; D004717:Endometrium / Q000187:drug effects / Q000378:metabolism*; D004958:Estradiol / Q000008:administration & dosage / Q000097:blood; D005260:Female; D005786:Gene Expression Regulation; D020249:Hormone Replacement Therapy; D006966:Hyperprolactinemia / Q000139:chemically induced / Q000235:genetics* / Q000378:metabolism; D008787:Metoclopramide; D051379:Mice; D010052:Ovariectomy; D011374:Progesterone / Q000008:administration & dosage / Q000097:blood; D020033:Protein Isoforms; D012333:RNA, Messenger / Q000378:metabolism; D011981:Receptors, Prolactin / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction
D020033:Protein Isoforms; D012333:RNA, Messenger; D011981:Receptors, Prolactin; D011374:Progesterone; D004958:Estradiol; D008787:Metoclopramide