The radial glia antibody RC2 recognizes a protein encoded by Nestin.
The RC2 antibody is widely used to label mouse radial glial cells in the developing central nervous system. While the antibody is known to recognize a 295-kDa intermediate filament proximal protein, the gene encoding the RC2 antigen remains to be identified. Here, we present evidences clearly demonstrating that Nestin encodes the RC2 antigen. First, the RC2 antigen and nestin have the same molecular weight and very similar tissue distribution. Second, genetic manipulations altering nestin expression also exert the same effect on the expression of the RC2 antigen. In particular, Nestin null mutation completely abolishes the RC2 immunoreactivity. Third, the expression of a truncated mouse nestin in Nestin-/- cells produces a small RC2 antigen whose size is the same to that of the truncated nestin. Furthermore, our data suggest that the RC2 antibody recognizes the C-terminal domain of nestin with unidentified posttranslational modification(s).
Park
Donghyun
D
Howard Hughes Medical Institute, Department of Human Genetics, University of Chicago, 929E, 57th, W503B, Chicago, IL 60637, USA. donghyun@uchicago.edu
Xiang
Andy Peng
AP
Zhang
Li
L
Mao
Frank Fuxiang
FF
Walton
Noah M
NM
Choi
Sun Shim
SS
Lahn
Bruce T
BT
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000941:Antigens / Q000235:genetics* / Q000276:immunology* / Q000378:metabolism; D018076:DNA, Complementary / Q000235:genetics; D007381:Intermediate Filament Proteins / Q000235:genetics* / Q000276:immunology* / Q000378:metabolism; D051379:Mice; D018345:Mice, Knockout; D009419:Nerve Tissue Proteins / Q000235:genetics* / Q000276:immunology* / Q000378:metabolism; D064231:Nestin; D009457:Neuroglia / Q000276:immunology*; D011499:Protein Processing, Post-Translational
D000911:Antibodies, Monoclonal; D000941:Antigens; D018076:DNA, Complementary; D007381:Intermediate Filament Proteins; C577484:NES protein, human; D009419:Nerve Tissue Proteins; C577485:Nes protein, mouse; D064231:Nestin