A cotton gene encodes a tonoplast aquaporin that is involved in cell tolerance to cold stress.
To enhance the survival probability in cold stress, plant cells often increase their cold- and freezing-tolerance in response to low, nonfreezing temperatures by expressing some cold-related genes. In present study, a cotton gene encoding tonoplast intrinsic protein (TIP) was isolated from a cotton seedling cDNA library, and designated as GhTIP1;1. GFP fluorescent microscopy indicated that GhTIP1;1 protein was localized to the vacuolar membrane. Assay on GhTIP1;1 expression in Xenopus laevis oocytes demonstrated that GhTIP1;1 protein displayed water channel activity and facilitated water transport to the cells. At normal conditions, GhTIP1;1 transcripts were predominantly accumulated in roots and hypocotyls, but less abundance in other tissues of cotton. The GhTIP1;1 expression was dramatically up-regulated in cotyledons, but down-regulated in roots within a few hours after cotton seedlings were cold-treated. Overexpression of GhTIP1;1 in yeast (Schizosaccharomyces pombe) significantly enhanced the cell survival probability, suggesting that the GhTIP1;1 protein is involved in cell freezing-tolerance.
Li
Deng-Di
DD
Hubei Key Laboratory of Genetic Regulation and Integrative Biology, College of Life Sciences, HuaZhong Normal University, Wuhan 430079, China.
Tai
Fu-Ju
FJ
Zhang
Ze-Ting
ZT
Li
Yang
Y
Zheng
Yong
Y
Wu
Yan-Feng
YF
Li
Xue-Bao
XB
D000595:Amino Acid Sequence; D000818:Animals; D020346:Aquaporins / Q000235:genetics* / Q000502:physiology*; D003080:Cold Temperature; D017124:Conserved Sequence; D005260:Female; D018506:Gene Expression Regulation, Plant; D003368:Gossypium / Q000235:genetics*; D049452:Green Fluorescent Proteins / Q000378:metabolism; D008565:Membrane Proteins / Q000235:genetics* / Q000302:isolation & purification; D008845:Microinjections; D008969:Molecular Sequence Data; D009865:Oocytes / Q000378:metabolism; D010802:Phylogeny; D010940:Plant Proteins / Q000235:genetics* / Q000302:isolation & purification; D018517:Plant Roots / Q000378:metabolism; D012568:Schizosaccharomyces / Q000235:genetics / Q000502:physiology; D017386:Sequence Homology, Amino Acid; D013347:Subcellular Fractions / Q000378:metabolism; D014617:Vacuoles / Q000235:genetics / Q000378:metabolism; D014982:Xenopus laevis / Q000378:metabolism
D020346:Aquaporins; D008565:Membrane Proteins; D010940:Plant Proteins; C077491:tonoplast intrinsic protein, plant; D049452:Green Fluorescent Proteins