title: Acetylcholinesterase inhibitory and antioxidant properties of Cyclotrichium niveum, Thymus praecox subsp. caucasicus var. caucasicus, Echinacea purpurea and E. pallida.
abstract: The dichloromethane, ethyl acetate, ethanol, and aqueous extracts of Cyclotrichium niveum (CN) and Thymus praecox subsp. caucasicus var. caucasicus (TP), Echinacea purpurea (EPU), and E. pallida (EPA) along with the essential oils of CN and TP were assessed for their anti-acetylcholinesterase (AChE) and antioxidant activities. AChE inhibition was estimated using spectrophotometric method of Ellman. Antioxidant activity was evaluated by 2,2-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging and ferrous ion-chelating power tests. Ferric-reducing antioxidant power (FRAP) of CN and TP were also tested. CN essential oil was found to contain isomenthone (56.21%) and pulegone (19.76%). The ethyl acetate (83.11-87.98%) and dichloromethane (73.45-84.02%) extracts of CN showed the highest AChE inhibition. The ethyl acetate and ethanol extracts of TP exerted significant DPPH scavenger effect. The water extracts of CN and TP and the chloroform extract of the aerial parts of EPU displayed the highest ferrous ion-chelating effect. The leaf and flower essential oils of TP had the best FRAP.
authors.affiliation: Department of Pharmacognosy, Faculty of Pharmacy, Gazi University, 06330 Ankara, Turkey. iorhan@gazi.edu.tr
authors.forename: I
authors.initials: I
authors.lastname: Orhan
authors.forename: F S
authors.initials: FS
authors.lastname: Senol
authors.forename: A R
authors.initials: AR
authors.lastname: Gülpinar
authors.forename: M
authors.initials: M
authors.lastname: Kartal
authors.forename: N
authors.initials: N
authors.lastname: Sekeroglu
authors.forename: M
authors.initials: M
authors.lastname: Deveci
authors.forename: Y
authors.initials: Y
authors.lastname: Kan
authors.forename: B
authors.initials: B
authors.lastname: Sener
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001713:Biphenyl Compounds / Q000737:chemistry; D002800:Cholinesterase Inhibitors / Q000494:pharmacology*; D020900:Echinacea / Q000737:chemistry*; D004593:Electrophorus; D016166:Free Radical Scavengers / Q000737:chemistry; D008401:Gas Chromatography-Mass Spectrometry; D007502:Iron Chelating Agents / Q000737:chemistry / Q000494:pharmacology; D019686:Lamiaceae / Q000737:chemistry*; D009822:Oils, Volatile / Q000737:chemistry / Q000494:pharmacology; D010084:Oxidation-Reduction; D010636:Phenols / Q000737:chemistry / Q000494:pharmacology; D010851:Picrates / Q000737:chemistry; D010936:Plant Extracts / Q000494:pharmacology; D012997:Solvents
chemical_list: D000975:Antioxidants; D001713:Biphenyl Compounds; D002800:Cholinesterase Inhibitors; D016166:Free Radical Scavengers; D007502:Iron Chelating Agents; D009822:Oils, Volatile; D010636:Phenols; D010851:Picrates; D010936:Plant Extracts; D012997:Solvents; C004931:1,1-diphenyl-2-picrylhydrazyl