title: HPLC enantioseparation of beta2-homoamino acids using crown ether-based chiral stationary phase.
abstract: RP high-performance liquid chromatographic methods were developed for the enantioseparation of eleven unusual beta(2)-homoamino acids. The underivatized analytes were separated on a chiral stationary phase containing (+)-(18-crown-6)-2,3,11,12-tetracarboxylic acid as chiral selector. The effects of organic (alcoholic) and acidic modifiers, the mobile phase composition and temperature on the separation were investigated. The structures of the substituents in the alpha-position of the analytes substantially influenced the retention and resolution. The elution sequence was determined in some cases: the S enantiomers eluted before the R enantiomers.
authors.affiliation: Department of Inorganic and Analytical Chemistry, University of Szeged, Szeged, Hungary.
authors.forename: Róbert
authors.initials: R
authors.lastname: Berkecz
authors.forename: István
authors.initials: I
authors.lastname: Ilisz
authors.forename: Aleksandra
authors.initials: A
authors.lastname: Misicka
authors.forename: Dagmara
authors.initials: D
authors.lastname: Tymecka
authors.forename: Ferenc
authors.initials: F
authors.lastname: Fülöp
authors.forename: Hee Jung
authors.initials: HJ
authors.lastname: Choi
authors.forename: Myung Ho
authors.initials: MH
authors.lastname: Hyun
authors.forename: Antal
authors.initials: A
authors.lastname: Péter
mesh_terms: D000596:Amino Acids / Q000032:analysis* / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D043844:Crown Ethers / Q000737:chemistry*; D015394:Molecular Structure; D013237:Stereoisomerism
chemical_list: D000596:Amino Acids; D043844:Crown Ethers