title: Up-regulated expression of peroxisome proliferator-activated receptor gamma in the hypothalamic-pituitary-adrenal axis of weaned pigs after Escherichia coli lipopolysaccharide challenge.
abstract: The expression of peroxisome proliferator-activated receptor gamma (PPARgamma) was investigated in the hypothalamic-pituitary-adrenal (HPA) axis of weaned pigs after injection with 100 microg/kg bodyweight Escherichia coli lipopolysaccharide (LPS) (n=6) and control pigs injected with sterile saline (n=6). LPS increased PPARgamma mRNA and protein expression in the hypothalamus (23.8 and 3.1-fold relative to controls, respectively), pituitary gland (9.2 and 2.0-fold, respectively) and adrenal gland (3.5 and 2.3-fold, respectively) (P<0.05). LPS also induced an increase in PPARgamma immunohistochemical staining in the hypothalamus (1.3-fold), adenohypophysis (1.3-fold), adrenal cortex (1.4-fold) and adrenal medulla (1.6-fold) (P<0.05). Concurrent with up-regulated expression of PPARgamma, LPS increased the concentrations of plasma corticotrophin-releasing hormone (2.1-fold) and adrenocorticotrophin (1.4-fold) (P<0.05). LPS also induced elevations of interleukin 6 and tumour necrosis factor alpha mRNA levels in the hypothalamus (4.0 and 3.2-fold, respectively), pituitary gland (20.7 and 5.1-fold, respectively) and adrenal gland (3.9 and 3.3-fold, respectively) (P<0.05). PPARgamma may play a role in the regulation of neuroendocrine responses associated with immunological stress in pigs.
authors.affiliation: Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan 430023, China. yulanflower@126.com
authors.forename: Yu Lan
authors.initials: YL
authors.lastname: Liu
authors.forename: Jun Xia
authors.initials: JX
authors.lastname: Shi
authors.forename: Jing
authors.initials: J
authors.lastname: Lu
authors.forename: Zheng Quan
authors.initials: ZQ
authors.lastname: Che
authors.forename: Hui Ling
authors.initials: HL
authors.lastname: Zhu
authors.forename: Yong Qing
authors.initials: YQ
authors.lastname: Hou
authors.forename: Yu Long
authors.initials: YL
authors.lastname: Yin
authors.forename: Sheng Jun
authors.initials: SJ
authors.lastname: Zhao
authors.forename: Bin Ying
authors.initials: BY
authors.lastname: Ding
authors.forename: Hong Ming
authors.initials: HM
authors.lastname: Liu
mesh_terms: D000818:Animals; D015153:Blotting, Western / Q000662:veterinary; D004926:Escherichia coli; D007030:Hypothalamo-Hypophyseal System / Q000187:drug effects* / Q000502:physiology; D007150:Immunohistochemistry / Q000662:veterinary; D008070:Lipopolysaccharides / Q000494:pharmacology*; D047495:PPAR gamma / Q000187:drug effects* / Q000378:metabolism*; D010913:Pituitary-Adrenal System / Q000187:drug effects* / Q000502:physiology; D011897:Random Allocation; D013552:Swine; D015854:Up-Regulation; D014886:Weaning
chemical_list: D008070:Lipopolysaccharides; D047495:PPAR gamma