title: Subproteome analysis of the neutrophil cytoskeleton.
abstract: Neutrophils play a key role in the early host-defense mechanisms due to their capacity to migrate into inflamed tissues and phagocytose microorganisms. The cytoskeleton has an essential role in these neutrophil functions, however, its composition is still poorly understood. We separately analyzed different cytoskeletal compartments: cytosolic skeleton, phagosome membrane skeleton, and plasma membrane skeleton. Using a proteomic approach, 138 nonredundant proteins were identified. Proteins not previously known to associate with the skeleton were: n-acetylglucosamine kinase, phosphoglycerate mutase 1, prohibitin, ficolin-1, phosphogluconate dehydrogenase, glucosidase, transketolase, major vault protein, valosin-containing protein, aldehyde dehydrogenase, and lung cancer-related protein-8 (LCRP8). The majority of these proteins can be classified as energy metabolism enzymes. Such a finding was interesting because neutrophil energy metabolism is unusual, mainly relying on glycolysis. The enrichment of phosphoglycerate mutase in cytosolic skeleton was additionally indicated by the use of Western blotting. This is the broadest subcellular investigation to date of the neutrophil cytoskeletal proteome and the first proteomic analysis in any cell type of the phagosome skeleton. The association of metabolic enzymes with cytoskeleton is suggestive of the importance of their localized enrichment and macromolecular organization in neutrophils.
authors.affiliation: Department of Medicine, Centre for Molecular Medicine, The Rayne Institute, University College London, London, UK.
authors.forename: Ping
authors.initials: P
authors.lastname: Xu
authors.forename: Mark
authors.initials: M
authors.lastname: Crawford
authors.forename: Michael
authors.initials: M
authors.lastname: Way
authors.forename: Jasminka
authors.initials: J
authors.lastname: Godovac-Zimmermann
authors.forename: Anthony W
authors.initials: AW
authors.lastname: Segal
authors.forename: Marko
authors.initials: M
authors.lastname: Radulovic
mesh_terms: D015153:Blotting, Western; D002462:Cell Membrane / Q000378:metabolism*; D003599:Cytoskeleton / Q000378:metabolism*; D003902:Detergents / Q000737:chemistry; D015180:Electrophoresis, Gel, Two-Dimensional; D020869:Gene Expression Profiling; D006801:Humans; D013058:Mass Spectrometry; D009504:Neutrophils / Q000378:metabolism*; D010588:Phagosomes / Q000378:metabolism*; D011506:Proteins / Q000032:analysis*; D040901:Proteomics / Q000379:methods*
chemical_list: D003902:Detergents; D011506:Proteins