title: Monocyte attachment to laminin in diabetes mellitus: The role of ATP.
abstract: Monocyte-extracellular matrix interactions have been implicated in atherosclerosis pathophysiology. Laminin, the main basement membrane protein contains cell binding domains that can be cryptic, presented only after protein modification. In the present study we evaluated monocyte attachment to laminin-1 in the presence of ATP. Monocytes were derived from either healthy volunteers or patients with diabetes mellitus type II. For the estimation of monocyte attachment to laminin the myeloperoxidase assay was used. Monocytes derived from diabetic patients, showed an increased ability to attach to laminin (p = 0.0055). The presence of ATP increased the attachment of control monocytes to laminin (p = 0.0022). On the contrary, the presence of ATP did not affect the attachment of monocytes derived from diabetic patients to laminin. Our results indicate a modified interaction between monocytes and laminin-1 in diabetes mellitus.
authors.affiliation: Department of Zoology, Aristotle University of Thessaloniki, Greece.
authors.forename: Elena
authors.initials: E
authors.lastname: Kostidou
authors.forename: Varvara
authors.initials: V
authors.lastname: Trachana
authors.forename: Konstantina
authors.initials: K
authors.lastname: Topouridou
authors.forename: Konstantinos
authors.initials: K
authors.lastname: Paletas
authors.forename: Apostolos
authors.initials: A
authors.lastname: Tsapas
authors.forename: Martha
authors.initials: M
authors.lastname: Kaloyianni
authors.forename: George
authors.initials: G
authors.lastname: Koliakos
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism*; D000328:Adult; D000368:Aged; D016022:Case-Control Studies; D002448:Cell Adhesion; D003920:Diabetes Mellitus / Q000378:metabolism*; D006801:Humans; D007797:Laminin / Q000378:metabolism*; D008875:Middle Aged; D009000:Monocytes / Q000166:cytology*
chemical_list: D007797:Laminin; D000255:Adenosine Triphosphate