title: Production, partial purification and characterization of organic solvent tolerant lipase from Burkholderia multivorans V2 and its application for ester synthesis.
abstract: Burkholderia multivorans V2 (BMV2) isolated from soil was found to produce an extracellular solvent tolerant lipase (6.477 U/mL). This lipase exhibited maximum stability in n-hexane retaining about 97.8% activity for 24h. After performing statistical optimization of medium components for lipase production, a 2.2-fold (14 U/mL) enhancement in the lipase production was observed. The crude lipase from BMV2 was partially purified by ultrafiltration and gel permeation chromatography with 24.64-fold purification. The K(m) and V(max) values for partially purified BMV2 lipase were found to be 1.56 mM and 5.62 micromoles/mg min. The metal ions Ca(2+), Mg(2+) and Mn(2+) had stimulatory effect on lipase activity, whereas Cu(2+), Fe(2+) and Zn(2+) strongly inhibited the lipase activity. EDTA and PMSF at 10mM concentration strongly inhibited the lipase activity. Non-ionic and anionic surfactants stimulated the lipase activity. BMV2 lipase was proved to be efficient in synthesis of ethyl butyrate ester under non-aqueous environment.
authors.affiliation: BRD School of Biosciences, Sardar Patel Maidan, Sardar Patel University, Vallabh Vidyanagar, Gujarat, India. vdandavate@gmail.com
authors.forename: Vrushali
authors.initials: V
authors.lastname: Dandavate
authors.forename: Jayesh
authors.initials: J
authors.lastname: Jinjala
authors.forename: Haresh
authors.initials: H
authors.lastname: Keharia
authors.forename: Datta
authors.initials: D
authors.lastname: Madamwar
mesh_terms: D001709:Biotechnology / Q000379:methods*; D019117:Burkholderia / Q000201:enzymology*; D002087:Butyrates / Q000737:chemistry; D002244:Carbon / Q000737:chemistry; D002625:Chemistry, Organic / Q000379:methods; D002845:Chromatography / Q000379:methods; D004952:Esters / Q000138:chemical synthesis* / Q000737:chemistry; D005374:Filtration; D007218:Industrial Microbiology / Q000379:methods; D007477:Ions; D007700:Kinetics; D008049:Lipase / Q000737:chemistry* / Q000302:isolation & purification*; D008670:Metals / Q000737:chemistry; D010802:Phylogeny; D012997:Solvents / Q000737:chemistry
chemical_list: D002087:Butyrates; D004952:Esters; D007477:Ions; D008670:Metals; D012997:Solvents; D002244:Carbon; D008049:Lipase; C045572:ethyl butyrate