Functional characterization of mesenchymal stem cells labeled with a novel PVP-coated superparamagnetic iron oxide.
Magnetic resonance imaging of cells labeled with superparamagnetic iron oxide (SPIO) could be a valuable tool for tracking transplanted cells in living organisms. Human bone marrow-derived mesenchymal stem cells (hBMMSC) were labeled with a novel polyvinyl pyrrolidone (PVP)-coated SPIO. Prussian blue staining and electron microscopy revealed that almost all of the cells were efficiently labeled with PVP-SPIO nanoparticles. There were no signs of cytotoxicity, even at concentrations of up to 1600 microg Fe/ml of the nanoparticles, and the labeled cells were successfully visualized by in vitro cellular MRI. In addition, there was no significant alteration of the phenotype or the adipo/osteo/chondrogenic differentiation potential of the cells. This was in contrast to Feridex IV labeling that led to the inhibition of hBMMSC chondrogenesis. Following intramuscular injection in a rabbit hind limb ischemia model, the intercellular migration of the labeled cells toward the ablated site was clearly tracked through in vivo MRI. The localization of the transplanted cells observed by MRI correlated well with postmortem histological studies. These results demonstrate that the novel PVP-SPIO nanoparticles appear to be efficient MR contrast agents and may enable non-invasive in vivo tracking of stem cells in experimental and clinical settings during cell therapy.
Reddy
Alavala Matta
AM
Department of Radiology, Chung-Ang University College of Medicine, Seoul, Republic of Korea.
Kwak
Byung Kook
BK
Shim
Hyung Jin
HJ
Ahn
Chiyoung
C
Cho
Sun Hang
SH
Kim
Byung Jin
BJ
Jeong
Sang Young
SY
Hwang
Sung-Joo
SJ
Yuk
Soon Hong
SH
D000818:Animals; D015415:Biomarkers; D016923:Cell Death; D002454:Cell Differentiation; D002470:Cell Survival; D005290:Ferric Compounds / Q000378:metabolism*; D006801:Humans; D008279:Magnetic Resonance Imaging; D008280:Magnetics; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism* / Q000648:ultrastructure; D019047:Phantoms, Imaging; D010641:Phenotype; D011205:Povidone / Q000378:metabolism*; D011817:Rabbits; D013194:Staining and Labeling
D015415:Biomarkers; D005290:Ferric Compounds; C000499:ferric oxide; D011205:Povidone