New ITS genotype of Cryptococcus gattii isolated from an AIDS patient in Brazil.
Based on combinations of nine variable nucleotides at nine different base positions in the ITS1-5.8S-ITS2 region, Cryptococcus gattii strains were classified into six genotypes. A new genotype of C. gattii, designated as ITS type 8, was isolated from an AIDS patient in Brazil. The ITS type 8 strain is closely related to the ITS type 4 strain, which has been frequently isolated in Brazil and the USA, but which shows ITS-signatured nucleotide difference at each nucleotide position. The ITS type 8 strain is also differentiated from all heretofore reported ITS types of C. gattii strains in the RAPD band patterns and IGS sequence information.
Kang
Yingqian
Y
Medical Mycology Research Center, Chiba University, 1-8-1, Inohana, Chuo-ku, Chiba-city, Chiba 260-8673, Japan.
Tanaka
Hiroko
H
Moretti
Maria Luiza
ML
Mikami
Yuzuru
Y
D017088:AIDS-Related Opportunistic Infections / Q000382:microbiology*; D000163:Acquired Immunodeficiency Syndrome / Q000382:microbiology*; D001938:Brazil; D003454:Cryptococcus / Q000145:classification / Q000235:genetics*; D016172:DNA Fingerprinting; D004271:DNA, Fungal / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000235:genetics; D005838:Genotype; D006801:Humans; D008297:Male; D010802:Phylogeny; D019105:Random Amplified Polymorphic DNA Technique; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D055815:Young Adult
D004271:DNA, Fungal; D021903:DNA, Ribosomal Spacer