Rapid identification of polyphenol C-glycosides from Swertia franchetiana by HPLC-ESI-MS-MS.
High-performance liquid chromatography coupled to positive ion electrospray ionization tandem mass spectrometry (MS) and diode array detection was employed to identify the polyphenol C-glycosides in the extract of Swertia franchetiana, a traditional Chinese/Tibetan herb. The neutral loss scan of the extract of S. franchetiana using the characteristic losses of 120 and 150 u provided a detailed profile of the polyphenol C-glycosides in the complex mixture. On-line UV spectroscopy along with MS-MS and MS-MS-MS mass spectra analysis produced with and without in-source collision induced dissociation was contributed to discriminate and identify the polyphenol C-glycosides. Three xanthone C-glycosides (i.e., mangiferin, isomangiferin, and 1,6,7-trihydroxyl-2-C-glucosexanthone) and three flavone C-glycosides (i.e., isoorientin, isovitexin, and swertisin) were tentatively identified. Isomangiferin and 1,6,7-trihydroxyl-2-C-glucosexanthone were for the first time found in this plant.
Sun
Yanguo
Y
Dalian Institute of Chemical Physics, Chinese Academy of Sciences, 457 Zhongshan Road, Dalian 116023, PR China.
Zhang
Xi
X
Xue
Xingya
X
Zhang
Yan
Y
Xiao
Hongbin
H
Liang
Xinmiao
X
D004156:Catechol Oxidase / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D047309:Flavones / Q000032:analysis; D006027:Glycosides; D009005:Monosaccharides / Q000032:analysis*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D029967:Swertia / Q000737:chemistry*; D053719:Tandem Mass Spectrometry / Q000379:methods*; D044004:Xanthones / Q000032:analysis
C540391:1,6,7-trihydroxyl-2-C-glucosexanthone; C438934:C-glycoside; D047309:Flavones; D006027:Glycosides; D009005:Monosaccharides; D044004:Xanthones; C013592:mangiferin; C061492:isomangiferin; D004156:Catechol Oxidase