Alterations to the protein profile of bladder carcinoma cell lines induced by plant extract MINA-05 in vitro.
Bladder cancer (BLCa) is a severe urological cancer of both men and women that commonly recurs and once invasive, is difficult to treat. MINA-05 (CK Life Sciences Int'l, Hong Kong) is a derivative of complex botanical extracts, shown to reduce cellular proliferation of bladder and prostate carcinomas. We tested the effects of MINA-05 against human BLCa cell sublines, B8, B8-RSP-GCK, B8-RSP-LN and C3, from a transitional cell carcinoma, grade IV, to determine the molecular targets of treatment by observing the cellular protein profile. Cells were acclimatised for 48 h then treated for 72 h with concentrations of MINA-05 reflecting 1/2 IC(50), IC(50) and 2 x IC(50) (n = 3) or with vehicle, (0.5% DMSO). Dose-dependant changes in protein abundance were detected and characterised using 2-dimensional electrophoresis and MS. We identified 10 proteins that underwent changes in abundance, pI and/or molecular mass in response to treatment. MINA-05 was shown to influence proteins across numerous functional classes including cytoskeletal proteins, energy metabolism proteins, protein degradation proteins and tumour suppressors, suggesting a global impact on these cell lines. This study implies that the ability of MINA-05 to retard cellular proliferation is attributed to its ability to alter cell cycling, metabolism, protein degradation and the cancer cell environment.
Nguyen-Khuong
Terry
T
Minomic Pty Ltd, Frenchs Forest, New South Wales, Australia.
White
Melanie Y
MY
Hung
Tzong-Tyng
TT
Seeto
Shona
S
Thomas
Melissa L
ML
Fitzgerald
Anna M
AM
Martucci
Carlos E
CE
Luk
Sharon
S
Pang
Shiu-Fu
SF
Russell
Pamela J
PJ
Walsh
Bradley J
BJ
D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D015180:Electrophoresis, Gel, Two-Dimensional; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects*; D006801:Humans; D009363:Neoplasm Proteins / Q000378:metabolism; D010751:Phosphopyruvate Hydratase / Q000378:metabolism; D032141:Schisandra; D013025:Glycine max; D001749:Urinary Bladder Neoplasms / Q000378:metabolism*; D027623:Yucca
D004365:Drugs, Chinese Herbal; D009363:Neoplasm Proteins; D010751:Phosphopyruvate Hydratase