Antibody recognition force microscopy shows that outer membrane cytochromes OmcA and MtrC are expressed on the exterior surface of Shewanella oneidensis MR-1.
Antibody recognition force microscopy showed that OmcA and MtrC are expressed on the exterior surface of living Shewanella oneidensis MR-1 cells when Fe(III), including solid-phase hematite (Fe(2)O(3)), was the terminal electron acceptor. OmcA was localized to the interface between the cell and mineral. MtrC displayed a more uniform distribution across the cell surface. Both cytochromes were associated with an extracellular polymeric substance.
Lower
Brian H
BH
School of Environment and Natural Resources, The Ohio State University, 210 Kottman Hall, 2021 Coffey Rd., Columbus, OH 43210, USA. Lower.30@osu.edu
Yongsunthon
Ruchirej
R
Shi
Liang
L
Wildling
Linda
L
Gruber
Hermann J
HJ
Wigginton
Nicholas S
NS
Reardon
Catherine L
CL
Pinchuk
Grigoriy E
GE
Droubay
Timothy C
TC
Boily
Jean-François
JF
Lower
Steven K
SK
D000906:Antibodies / Q000378:metabolism; D001426:Bacterial Proteins / Q000032:analysis*; D003574:Cytochrome c Group / Q000032:analysis*; D003580:Cytochromes / Q000032:analysis*; D005290:Ferric Compounds / Q000378:metabolism; D008565:Membrane Proteins / Q000032:analysis*; D018625:Microscopy, Atomic Force; D020592:Shewanella / Q000737:chemistry*
D000906:Antibodies; D001426:Bacterial Proteins; D003574:Cytochrome c Group; D003580:Cytochromes; D005290:Ferric Compounds; D008565:Membrane Proteins; C000499:ferric oxide; C433527:MtrC protein, Shewanella