Interleukin-6 induces proinflammatory signaling in Schwann cells: a high-throughput analysis.
Interleukin-6 plays an important role in peripheral nerve regeneration. We recently reported that IL-6 targets Schwann cells in the peripheral nerve for its function. In this study, we analyzed genes whose expression is regulated by IL-6 in a cell line derived from Schwann cells, the peripheral glia, using the Illumina gene microarray. At measurements 3 and 12h after IL-6 treatment, 35 genes were found to be upregulated by IL-6. Most upregulated genes were proinflammatory genes that are known to be induced in inflammatory conditions. Interestingly, the expression of immunoproteasome subunits was upregulated by IL-6 in Schwann cells. Treatment with forskolin, an agent that mimics axonal signaling, suppressed the expression of IL-6-inducible genes. Finally, we found for the first time that sciatic nerve injury induced immunoproteasome expression in vivo. These findings indicate that IL-6 is involved in peripheral nerve regeneration by regulating proinflammatory signaling in Schwann cells.
Lee
Hyun Kyoung
HK
Department of Physiology, College of Medicine, Dong-A University, Seo-Gu, Busan, South Korea.
Wang
Lih
L
Shin
Yoon Kyung
YK
Lee
Kyu Yeol
KY
Suh
Duk Joon
DJ
Park
Hwan Tae
HT
D000818:Animals; D045744:Cell Line, Tumor; D005576:Colforsin / Q000378:metabolism; D020869:Gene Expression Profiling; D007249:Inflammation / Q000235:genetics / Q000276:immunology*; D015850:Interleukin-6 / Q000494:pharmacology / Q000502:physiology*; D051379:Mice; D020411:Oligonucleotide Array Sequence Analysis; D051381:Rats; D012583:Schwann Cells / Q000187:drug effects / Q000276:immunology*; D012584:Sciatic Nerve / Q000276:immunology / Q000293:injuries; D015398:Signal Transduction / Q000235:genetics / Q000276:immunology; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism; D015854:Up-Regulation
D015850:Interleukin-6; D014409:Tumor Necrosis Factor-alpha; D005576:Colforsin