title: Concentrations of danofloxacin 18% solution in plasma, milk and tissues after subcutaneous injection in dairy cows.
abstract: Danofloxacin is a fluoroquinolone developed for use in veterinary medicine. Its concentrations and pharmacokinetic profile in plasma, milk and tissues of lactating dairy cows were determined, and its milk withdrawal time (WT) calculated. Twenty-one dairy cows received a single subcutaneous administration of 18% mesylate danofloxacin salt (6 mgkg(-1)). Plasma and milk samples were obtained at different times until 48 h. Groups of three animals were sacrificed at different post-administration times and tissue samples (mammary gland, uterus, duodenum, jejunum, ileum, colon and mesenteric lymph nodes) obtained. Danofloxacin concentrations were determined by liquid chromatography with fluorescence detection. The milk WT was calculated by the Time to Safe Concentration method (Software WTM 1.4, EMEA). Danofloxacin was rapidly absorbed and its distribution from plasma to all sampled tissues and milk was extensive. Milk and tissues concentrations were several times above those found in plasma. Plasma area under the curve (AUCp) was 9.69 microghmL(-1) and its elimination half life (T(beta)(1/2)) was 12.53 h. AUC values for the various tissues and milk greatly exceeded AUCp. T(beta)(1/2) from milk and tissues ranged between 4.57 and 21.91 h and the milk withdrawal time was 73.48 h. The reported results support the potential use of danofloxacin in the treatment of mastitis and other infections in milk cows with 3 days of withdrawal.
authors.affiliation: Pharmacology Department, Faculty of Veterinary Science, Universidad Nacional de La Plata, CC 296, 1900 La Plata, Buenos Aires, Argentina.
authors.forename: N
authors.initials: N
authors.lastname: Mestorino
authors.forename: M L
authors.initials: ML
authors.lastname: Marchetti
authors.forename: E
authors.initials: E
authors.lastname: Turic
authors.forename: J
authors.initials: J
authors.lastname: Pesoa
authors.forename: J
authors.initials: J
authors.lastname: Errecalde
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000008:administration & dosage / Q000097:blood / Q000493:pharmacokinetics*; D019540:Area Under Curve; D002417:Cattle / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003612:Dairying; D004350:Drug Residues / Q000032:analysis / Q000302:isolation & purification; D005260:Female; D005456:Fluorescent Dyes / Q000032:analysis; D024841:Fluoroquinolones / Q000008:administration & dosage / Q000097:blood / Q000493:pharmacokinetics*; D006207:Half-Life; D007279:Injections, Subcutaneous; D007774:Lactation; D008892:Milk / Q000737:chemistry*; D011247:Pregnancy; D014020:Tissue Extracts / Q000737:chemistry
chemical_list: D000900:Anti-Bacterial Agents; D005456:Fluorescent Dyes; D024841:Fluoroquinolones; D014020:Tissue Extracts; C068581:danofloxacin