Influence of two Pt(IV) complexes on viability, apoptosis and cell cycle of B16 mouse melanoma tumors.
Several platinum(IV) complexes are showing considerable promise in initial trials, producing reactive intermediates that then interact with DNA.


OBJECTIVE
To perform in vitro study of two new platinum(IV) complexes cytotoxic effect on B16 mouse melanoma cells.


METHODS
PtCl(4)(dbtp)(2) and PtCl(2)(6mp)(2) complexes were prepared. PtCl(4)(dbtp)(2) was created as modification of PtCl(4)(dmtp) test previously. Apoptosis and necrosis were examined using flow cytometry, upon Annexin V/PI staining.


RESULTS
LC(10), LC(50) and LC(90) parameters established for PtCl(4)(dbtp)(2) were as following: 2.6, 17.0, 58.0 mumol/L. However LC(10) and LC(50) established for PtCl(2)(6mp)(2) were 1.2 and 14.0 micromol/l respectively. The both complexes induced apoptosis. PtCl(2)(6mp)(2) induced cell cycle arrest in G0/G1, while PtCl(4)(dbtp)(2) - in S-phase.


CONCLUSIONS
PtCl(4)(dbtp)(2) appeared to be more cytotoxic against B16 cells than PtCl(2)(6mp)(2). Apoptosis was the main mechanism of cell loss in cultures incubated with both tested complexes.
Kaznica
A
A
Chair of Medical Biology, Nicolaus Copernicus University, Bydgoszcz 85-092, Poland. a.kaznica@wp.pl
Drewa
T
T
Lakomska
I
I
Ryta-Stamirowska
P
P
Debski
R
R
Styczynski
J
J
Drewa
G
G
Szlyk
E
E
D000818:Animals; D000970:Antineoplastic Agents / Q000737:chemistry / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D002453:Cell Cycle / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D008546:Melanoma, Experimental / Q000188:drug therapy*; D051379:Mice; D009944:Organoplatinum Compounds / Q000737:chemistry / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
D000970:Antineoplastic Agents; D009944:Organoplatinum Compounds