Iterative in situ click chemistry creates antibody-like protein-capture agents.
Special agents for protein capture: Iterative in situ click chemistry (see scheme for the tertiary ligand screen) and the one-bead-one-compound method for the creation of a peptide library enable the fragment-based assembly of selective high-affinity protein-capture agents. The resulting ligands are water-soluble and stable chemically, biochemically, and thermally. They can be produced in gram quantities through copper(I)-catalyzed cycloaddition.
Agnew
Heather D
HD
Division of Chemistry and Chemical Engineering, California Institute of Technology, 1200 East California Boulevard, Pasadena, CA 91125, USA.
Rohde
Rosemary D
RD
Millward
Steven W
SW
Nag
Arundhati
A
Yeo
Woon-Seok
WS
Hein
Jason E
JE
Pitram
Suresh M
SM
Tariq
Abdul Ahad
AA
Burns
Vanessa M
VM
Krom
Russell J
RJ
Fokin
Valery V
VV
Sharpless
K Barry
KB
Heath
James R
JR
D000906:Antibodies / Q000737:chemistry; D002384:Catalysis; D003300:Copper / Q000737:chemistry; D008024:Ligands; D019151:Peptide Library; D010455:Peptides / Q000737:chemistry; D011485:Protein Binding; D011506:Proteins / Q000737:chemistry*; D014230:Triazoles / Q000737:chemistry*
D000906:Antibodies; D008024:Ligands; D019151:Peptide Library; D010455:Peptides; D011506:Proteins; D014230:Triazoles; D003300:Copper