title: [Effects of yougui drink on the osteogenic differentiation of mesenchymal stem cells in the proximal femur in steroid-induced avascular necrosis of femoral head].
abstract: OBJECTIVE
  To investigate the effects of Yougui drink on osteogenic differentiation of bone mesenchymal stem cells (BMSCs) in the proximal femur in steroid-induced avascular necrosis of femoral head in vitro.
  
  
  METHODS
  The BMSCs were induced to osteoblasts in vitro with a conditional medium. The cells cultured with Yougui drink serum were compared with the ordinary one, which both were examined by microscopy, MTU method, ALP and calcium node.
  
  
  RESULTS
  Microscopy and MTU studies showed that Yougui drink could improve proliferation of the osteoblasts. ALP and calcium node studies showed that Yougui drink could improve the activity of the osteoblasts (P < 0.01).
  
  
  CONCLUSIONS
  Yougui drink has positive effects on osteogenic differentiation of mesenchymal stem cells in the proximal femur in steroid-induced avascular necrosis of femoral head in vitro.
authors.affiliation: The First Hospital Affiliated to Wenzhou Medical College, Wenzhou 325000, Zhejiang, China. zjwyg@126.com
authors.forename: Yun-gang
authors.initials: YG
authors.lastname: Wu
authors.forename: Lu-wei
authors.initials: LW
authors.lastname: Xiao
authors.forename: Pei-jian
authors.initials: PJ
authors.lastname: Tong
mesh_terms: D002454:Cell Differentiation; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000494:pharmacology*; D005260:Female; D005271:Femur Head Necrosis / Q000188:drug therapy* / Q000503:physiopathology; D006801:Humans; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000187:drug effects; D008875:Middle Aged; D010006:Osteoblasts / Q000166:cytology* / Q000187:drug effects; D010020:Osteonecrosis; D013256:Steroids / Q000494:pharmacology*
chemical_list: D004365:Drugs, Chinese Herbal; D013256:Steroids