title: Expression of estrogenicity genes in a lineage cell culture model of human breast cancer progression.
abstract: TaqMan Gene Expression assays were used to profile the mRNA expression of estrogen receptor (ERalpha and ERbeta) and estrogen metabolism enzymes including cytosolic sulfotransferases (SULT1E1, SULT1A1, SULT2A1, and SULT2B1), steroid sulfatase (STS), aromatase (CYP19), 17beta-hydroxysteroid dehydrogenases (17betaHSD1 and 2), CYP1B1, and catechol-O-methyltransferase (COMT) in an MCF10A-derived lineage cell culture model for basal-like human breast cancer progression and in ERalpha-positive luminal MCF7 breast cancer cells. Low levels of ERalpha and ERbeta mRNA were present in MCF10A-derived cell lines. SULT1E1 mRNA was more abundant in confluent relative to subconfluent MCF10A cells, a non-tumorigenic proliferative breast disease cell line. SULT1E1 was also expressed in preneoplastic MCF10AT1 and MCF10AT1K.cl2 cells, but was markedly repressed in neoplastic MCF10A-derived cell lines as well as in MCF7 cells. Steroid-metabolizing enzymes SULT1A1 and SULT2B1 were only expressed in MCF7 cells. STS and COMT were widely detected across cell lines. Pro-estrogenic 17betaHSD1 mRNA was most abundant in neoplastic MCF10CA1a and MCF10DCIS.com cells, while 17betaHSD2 mRNA was more prominent in parental MCF10A cells. CYP1B1 mRNA was most abundant in MCF7 cells. Treatment with the histone deacetylase inhibitor trichostatin A (TSA) induced SULT1E1 and CYP19 mRNA but suppressed CYP1B1, STS, COMT, 17betaHSD1, and 17betaHSD2 mRNA in MCF10A lineage cell lines. In MCF7 cells, TSA treatment suppressed ERalpha, CYP1B1, STS, COMT, SULT1A1, and SULT2B1 but induced ERbeta, CYP19 and SULT2A1 mRNA expression. The results indicate that relative to the MCF7 breast cancer cell line, key determinants of breast estrogen metabolism are differentially regulated in the MCF10A-derived lineage model for breast cancer progression.
authors.affiliation: Institute of Environmental Health Sciences, Wayne State University, Detroit, MI, 48201, USA.
authors.forename: Jiaqi
authors.initials: J
authors.lastname: Fu
authors.forename: Amy M
authors.initials: AM
authors.lastname: Weise
authors.forename: Josie L
authors.initials: JL
authors.lastname: Falany
authors.forename: Charles N
authors.initials: CN
authors.lastname: Falany
authors.forename: Bryan J
authors.initials: BJ
authors.lastname: Thibodeau
authors.forename: Fred R
authors.initials: FR
authors.lastname: Miller
authors.forename: Thomas A
authors.initials: TA
authors.lastname: Kocarek
authors.forename: Melissa
authors.initials: M
authors.lastname: Runge-Morris
mesh_terms: D015067:17-Hydroxysteroid Dehydrogenases / Q000096:biosynthesis / Q000378:metabolism; D001141:Aromatase / Q000096:biosynthesis / Q000378:metabolism; D001189:Aryl Hydrocarbon Hydroxylases / Q000096:biosynthesis / Q000378:metabolism; D015153:Blotting, Western; D001943:Breast Neoplasms / Q000235:genetics* / Q000378:metabolism*; D002394:Catechol O-Methyltransferase / Q000096:biosynthesis / Q000378:metabolism; D045744:Cell Line, Tumor; D065633:Cytochrome P-450 CYP1B1; D018450:Disease Progression; D004791:Enzyme Inhibitors / Q000494:pharmacology; D047628:Estrogen Receptor alpha / Q000235:genetics* / Q000378:metabolism; D047629:Estrogen Receptor beta / Q000235:genetics* / Q000378:metabolism; D004967:Estrogens / Q000378:metabolism*; D005260:Female; D015870:Gene Expression; D006801:Humans; D012333:RNA, Messenger / Q000032:analysis; D043266:Steryl-Sulfatase / Q000096:biosynthesis / Q000378:metabolism; D015238:Sulfotransferases / Q000096:biosynthesis / Q000378:metabolism; D014162:Transfection
chemical_list: D004791:Enzyme Inhibitors; D047628:Estrogen Receptor alpha; D047629:Estrogen Receptor beta; D004967:Estrogens; D012333:RNA, Messenger; D015067:17-Hydroxysteroid Dehydrogenases; C044256:3 (or 17)-beta-hydroxysteroid dehydrogenase; D001141:Aromatase; D001189:Aryl Hydrocarbon Hydroxylases; C585496:CYP1B1 protein, human; D065633:Cytochrome P-450 CYP1B1; D002394:Catechol O-Methyltransferase; D015238:Sulfotransferases; D043266:Steryl-Sulfatase