title: [Quantitative evaluation of residual endodontic microorganisms after mechanical root canal preparation different chemical preparations by real-time PCR].
abstract: OBJECTIVE
  To establish a quick, sensitive method for quantifying root canal flora and investigate the effects of different root canal preparations on the pathogenic bacteria at RNA level.
  
  
  METHODS
  A total of 24 single-rooted teeth with chronic apical periodontitis were selected and prepared using 3% H2O2 combined with 1% NaClO, EDTA combined with 3% H(2)O(2),1% NaClO, respectively,the samples were taken before and after root canal preparation. After isolation of total RNA from the root canal samples, cDNA was synthesized by reverse transcription, and detected by real-time PCR. The data were analyzed with SAS 6.12 software package.
  
  
  RESULTS
  The number of bacteria in the root canal reduced dramatically after mechanical preparation and irrigated using 3% H(2)O(2) and 1% NaClO(P<0.01). Further combined with EDTA, its effect was better than that of simply irrigated using 3% H(2)O(2) and 1% NaClO(P<0.05).
  
  
  CONCLUSIONS
  Real-time PCR can be employed in the identification of bacteria flora in the root canal, both methods of root canal preparation can effectively reduce the number of bacteria flora.
authors.affiliation: Department of Endodontics and Operative Dentistry, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, China. jiangyuntao1974@163.com
authors.forename: Yun-tao
authors.initials: YT
authors.lastname: Jiang
authors.forename: Wen-wei
authors.initials: WW
authors.lastname: Xia
authors.forename: Jing-ping
authors.initials: JP
authors.lastname: Liang
authors.forename: Shan-fen
authors.initials: SF
authors.lastname: Ma
authors.forename: Pei-fang
authors.initials: PF
authors.lastname: Yan
mesh_terms: D003786:Dental Pulp Cavity; D006801:Humans; D006861:Hydrogen Peroxide; D010485:Periapical Periodontitis; D060888:Real-Time Polymerase Chain Reaction; D012388:Root Canal Irrigants; D018915:Root Canal Preparation; D012390:Root Canal Therapy
chemical_list: D012388:Root Canal Irrigants; D006861:Hydrogen Peroxide