[Effect of resveratrol-induced FasL up-regulation on the apoptosis of pancreatic acinar cells in rats with severe acute pancreatitis].
OBJECTIVE
To investigate the effect of resveratrol on the apoptosis of pancreatic acinar cells in rats with severe acute pancreatitis (SAP) and explore the mechanism of such effect.


METHODS
SD rats with 3.5% sodium taurocholate-induced SAP were treated with resveratrol, and the serum amylase was detected with automatic biochemistry analyzer. The apoptosis of the pancreatic acinar cells in the rats was detected by TUNEL assay, and the expression of Fas and FasL genes was determined by RT-PCR and Western blotting. The pathological changes of the pancreas were observed under optical microscope.


RESULTS
Compared with SAP group, the resveratrol-treated rats showed obviously decreased serum amylase and scores for pancreatic histopathological lesions. Resveratrol treatment significantly increased the apoptotic indices of pancreatic acinar cells and the levels of FasL mRNA and protein in rats with SAP.


CONCLUSIONS
Resveratrol produces important therapeutic effect on SAP in rats by inducing pancreatic acinar cell apoptosis possibly as a result of up-regulated FasL gene expression.
Li
Zhen-Dong
ZD
Department of Hepatobiliary Surgery, First Affiliated Hospital of Ji'nan University, Guangzhou, China. victor7922@163.com
Ma
Qing-Yong
QY
Luo
Yu-Hong
YH
D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D053222:Fas Ligand Protein / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D008297:Male; D046790:Pancreas, Exocrine / Q000473:pathology*; D019283:Pancreatitis, Acute Necrotizing / Q000139:chemically induced / Q000188:drug therapy* / Q000473:pathology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D000077185:Resveratrol; D013267:Stilbenes / Q000627:therapeutic use*; D013656:Taurocholic Acid; D015854:Up-Regulation
D053222:Fas Ligand Protein; C505662:Faslg protein, rat; D012333:RNA, Messenger; D013267:Stilbenes; D013656:Taurocholic Acid; D000077185:Resveratrol