title: An in vitro study of electrically active hydroxyapatite-barium titanate ceramics using Saos-2 cells.
abstract: Electrically active ceramics are of interest as bone graft substitute materials. This study investigated the ferroelectric properties of hydroxyapatite-barium titanate (HABT) composites and the behaviour of osteoblast-like cells seeded on their surfaces. A piezoelectric coefficient (d(33)) of 57.8 pCN(-1) was observed in HABT discs prepared for cell culture. The attachment, proliferation, viability, morphology and metabolic activity of cells cultured on unpoled HABT were comparable to those observed on commercially available hydroxyapatite at all time points. No indication of the cytotoxicity of HABT was detected. At one day after seeding, cell attachment was modified on both the positive and negative surfaces of poled HABT. After longer incubations, all parameters observed were comparable on poled and unpoled ceramics. The results indicate that HABT ceramics are biocompatible in the short term in vitro and that further investigation of cell responses to these materials under mechanical load and at longer incubation times is warranted.
authors.affiliation: Department of Mechanical Engineering, Centre for Orthopaedic Biomechanics, University of Bath, Bath, UK.
authors.forename: Frances R
authors.initials: FR
authors.lastname: Baxter
authors.forename: Irene G
authors.initials: IG
authors.lastname: Turner
authors.forename: Christopher R
authors.initials: CR
authors.lastname: Bowen
authors.forename: Jonathan P
authors.initials: JP
authors.lastname: Gittings
authors.forename: Julian B
authors.initials: JB
authors.lastname: Chaudhuri
mesh_terms: D017609:Barium Compounds / Q000737:chemistry*; D002448:Cell Adhesion; D018929:Cell Culture Techniques; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D002516:Ceramics / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology; D017886:Durapatite / Q000737:chemistry*; D004553:Electric Conductivity; D006801:Humans; D006882:Hydroxyapatites / Q000737:chemistry; D008422:Materials Testing; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects / Q000378:metabolism / Q000502:physiology; D013499:Surface Properties; D014025:Titanium / Q000737:chemistry*
chemical_list: D017609:Barium Compounds; D006882:Hydroxyapatites; C024547:barium titanate(IV); C041297:barium hydroxyapatite; D017886:Durapatite; D014025:Titanium