O-GlcNAc inhibits interaction between Sp1 and Elf-1 transcription factors.
The novel protein modification, O-linked N-acetylglucosamine (O-GlcNAc), plays an important role in various aspects of cell regulation. Although most of nuclear transcription regulatory factors are modified by O-GlcNAc, O-GlcNAc effects on transcription remain largely undefined yet. In this study, we show that O-GlcNAc inhibits a physical interaction between Sp1 and Elf-1 transcription factors, and negatively regulates transcription of placenta and embryonic expression oncofetal protein gene (Pem). These findings suggest that O-GlcNAc inhibits Sp1-mediated gene transcription possibly by interrupting Sp1 interaction with its cooperative factor.
Lim
Kihong
K
School of Life Sciences and Biotechnology, Korea University, 5-1 Anam-dong, Seongbuk-gu, Seoul 136-701, Republic of Korea.
Chang
Hyo-Ihl
HI
D000117:Acetylglucosamine; D000818:Animals; D045744:Cell Line, Tumor; D036383:Ephrin-A2 / Q000037:antagonists & inhibitors* / Q000378:metabolism; D005786:Gene Expression Regulation; D018398:Homeodomain Proteins / Q000235:genetics*; D006801:Humans; D051379:Mice; D051381:Rats; D016329:Sp1 Transcription Factor / Q000037:antagonists & inhibitors* / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics*; D014158:Transcription, Genetic
D000117:Acetylglucosamine; D036383:Ephrin-A2; D018398:Homeodomain Proteins; D016329:Sp1 Transcription Factor; D014157:Transcription Factors; C498048:Rhox5 protein, mouse