title: Novel CTX-M {beta}-lactamase genotype distribution and spread into multiple species of Enterobacteriaceae in Changsha, Southern China.
abstract: OBJECTIVE
  The aim of this study was to undertake a survey of the occurrence of CTX-M and SHV extended-spectrum beta-lactamase (ESBL) genotypes in Enterobacteriaceae from Hunan Province, China.
  
  
  METHODS
  Clinical isolates (425) from three major hospitals in Changsha, Hunan Province, were collected between October 2004 and July 2005, and their antimicrobial susceptibilities of the genotype of bla(CTX-M) and bla(SHV) were determined. Random amplified polymorphic DNA was used to characterize the clonality of all of the isolates.
  
  
  RESULTS
  The overall rate of ESBL-positive isolates was 33.4% (142/425). The dominant ESBLs were CTX-M types, and were found in 109/142 (76.8%) isolates comprising seven different genera/species, namely Escherichia coli, Klebsiella pneumoniae, Enterobacter cloacae, Enterobacter aerogenes, Citrobacter freundii, Proteus vulgaris and Providencia stuartii. The most common bla(CTX-M) genotypes were bla(CTX-M-14) (47.7%), bla(CTX-M-3) (29.4%) and bla(CTX-M-15) (17.4%). A novel gene derived from bla(CTX-M-15), bla(CTX-M-82) (Ala-40-->Pro), was identified.
  
  
  CONCLUSIONS
  The dominant ESBL genotype in Hunan Province was bla(CTX-M). The high prevalence (17.4%) of bla(CTX-M-15) has not previously been reported from China. Our results identify that an epidemic of bla(CTX-M) in Changsha, Hunan Province, has evolved with the appearance and spread of bla(CTX-M-15) against the dominant genotypes bla(CTX-M-14) and bla(CTX-M-3.) The worldwide dominance of bla(CTX-M-15) could be poised to spread to China, displacing the current prevailing genotypes.
authors.affiliation: Xiangya Hospital, Central South University, Changsha, Hunan, P. R. China. liuwenen@hotmail.com
authors.forename: Wen'en
authors.initials: W
authors.lastname: Liu
authors.forename: Lamei
authors.initials: L
authors.lastname: Chen
authors.forename: Hongling
authors.initials: H
authors.lastname: Li
authors.forename: Huili
authors.initials: H
authors.lastname: Duan
authors.forename: Yunli
authors.initials: Y
authors.lastname: Zhang
authors.forename: Xianghui
authors.initials: X
authors.lastname: Liang
authors.forename: Xian
authors.initials: X
authors.lastname: Li
authors.forename: Mingxiang
authors.initials: M
authors.lastname: Zou
authors.forename: Li
authors.initials: L
authors.lastname: Xu
authors.forename: Peter M
authors.initials: PM
authors.lastname: Hawkey
mesh_terms: D015373:Bacterial Typing Techniques; D002681:China / Q000453:epidemiology; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004755:Enterobacteriaceae / Q000201:enzymology* / Q000302:isolation & purification; D004756:Enterobacteriaceae Infections / Q000453:epidemiology / Q000382:microbiology; D005838:Genotype; D006761:Hospitals; D006801:Humans; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D019105:Random Amplified Polymorphic DNA Technique; D017422:Sequence Analysis, DNA; D001618:beta-Lactamases / Q000096:biosynthesis / Q000235:genetics*
chemical_list: D004269:DNA, Bacterial; D001618:beta-Lactamases