title: Application of SNPs in forensic casework: identification of pathological and autoptical specimens due to sample mix-up.
abstract: The analysis of single nucleotide polymorphisms (SNPs) together with conventional short tandem repeat (STR) and mitochondrial DNA (mtDNA) typing provide a forensic genetic approach for the identification of pathological and autoptical specimens in cases where the average length of DNA fragments is shorter than 150 bp in highly degraded samples. We applied a forensic genetic approach to digesta accidentally left after a training autopsy. PCR products were not amplified from samples containing the STR loci or common sequences used for mtDNA typing. The application of SNPs and deletion polymorphisms provides an alternative approach for DNA typing analysis.
authors.affiliation: Department of Forensic Medicine, National Defense Medical College, 3-2 Namiki, Tokorozawa, Saitama 359-8513, Japan.
authors.forename: Yuzo
authors.initials: Y
authors.lastname: Takada
authors.forename: Tomoharu
authors.initials: T
authors.lastname: Tokutomi
authors.forename: Jun
authors.initials: J
authors.lastname: Kanetake
authors.forename: Masahiro
authors.initials: M
authors.lastname: Mukaida
mesh_terms: D053836:DNA Degradation, Necrotic; D016172:DNA Fingerprinting; D053938:DNA Fragmentation; D004272:DNA, Mitochondrial / Q000235:genetics*; D005819:Genetic Markers; D005838:Genotype; D006801:Humans; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide; D020080:Tandem Repeat Sequences
chemical_list: D004272:DNA, Mitochondrial; D005819:Genetic Markers