Expanding the repertoire of fluorescent calcium sensors.
Fluorescent indicators for calcium are incredibly powerful because they enable researchers to watch the movement of calcium ions in real time in living cells. The popular small molecule indicator Indo-1 has now been targeted to a defined location, namely, the nucleus of muscle cells, using SNAP-tag technology. This combination of a chemical probe with genetic targeting expands the available options for measuring local calcium events in cells.
Palmer
Amy E
AE
Department of Chemistry and Biochemistry, University of Colorado, UCB 215, Boulder, Colorado 80309, USA. amy.palmer@colorado.edu
D000818:Animals; D002118:Calcium / Q000032:analysis* / Q000378:metabolism; D002477:Cells / Q000378:metabolism; D004721:Endoplasmic Reticulum / Q000378:metabolism; D005456:Fluorescent Dyes / Q000737:chemistry*; D006801:Humans; D007211:Indoles / Q000737:chemistry; D008856:Microscopy, Fluorescence; D008928:Mitochondria / Q000378:metabolism
D005456:Fluorescent Dyes; D007211:Indoles; C048960:indo-1; D002118:Calcium