Local and systemic delivery of siRNAs for oligonucleotide therapy.
RNA interference (RNAi) is a relatively new found phenomenon of posttranscriptional gene silencing to regulate the expression of multiple genes involved in a wide range of biological processes. The gene-silencing technology via RNAi has also been developed into a commonly anti-gene method. Furthermore, in vivo data indicate that small interfering RNAs (siRNAs) may be used to treat human diseases. However, the most challenging issue to a successful in vivo application is the development of a delivery system that can transport siRNA molecules into the tissues and/or the cells of interest. Also, the evaluation of siRNA potency in vivo is central for the selection of therapeutic siRNAs. In this chapter, the effects of atelocollagen-delivered siRNAs in live animals were monitored using bioluminescence imaging.
Takeshita
Fumitaka
F
Section for Studies on Metastasis, National Cancer Center Research Institute, Tokyo, Japan.
Hokaiwado
Naomi
N
Honma
Kimi
K
Banas
Agnieszka
A
Ochiya
Takahiro
T
D000818:Animals; D003094:Collagen / Q000008:administration & dosage*; D003952:Diagnostic Imaging; D016503:Drug Delivery Systems; D015316:Genetic Therapy / Q000379:methods*; D006801:Humans; D008297:Male; D051379:Mice; D008819:Mice, Nude; D009369:Neoplasms / Q000628:therapy; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000008:administration & dosage*
D034741:RNA, Small Interfering; C059821:atelocollagen; D003094:Collagen