Rapid screening for anthocyanins and anthocyanin dimers in crude grape extracts by high performance liquid chromatography coupled with diode array detection and tandem mass spectrometry.
A rapid and efficient method using high performance liquid chromatography coupled with diode array detection and tandem mass spectrometry (HPLC-DAD-MS/MS) for fast screening large numbers of anthocyanins and anthocyanin dimers in different grape skin extracts, without further sample clean-up procedures, was developed. A good separation of most detected anthocyanins was achieved in a run time of 15 min. Identification of anthocyanin pigments required a combination of several information: UV-vis spectra, MS and MS/MS spectra, and elution pattern. Many compounds have been here detected for the first time and their structures tentatively elucidated.
Pati
Sandra
S
Department of Food Science, University of Foggia-Via Napoli 25, Foggia, Italy. s.pati@unifg.it
Liberatore
Maria Teresa
MT
Gambacorta
Giuseppe
G
Antonacci
Donato
D
La Notte
Ennio
E
D000872:Anthocyanins / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D019281:Dimerization; D010936:Plant Extracts / Q000737:chemistry; D053719:Tandem Mass Spectrometry / Q000295:instrumentation / Q000379:methods*; D027843:Vitis / Q000737:chemistry*
D000872:Anthocyanins; D010936:Plant Extracts