Construction of EGFP-tagged rBCG of E.tenella and distribution in chickens.
Chicken coccidiosis is a major parasitic disease with substantial economic burden to the poultry industry. Enhanced green fluorescent protein (EGFP) tagged recombinant Bacille Calmette-Guerin (rBCG), as a fusion protein with coccidian rhomboid antigen was constructed to track rBCG in vivo in chickens in this study. Immunization of chickens with one dose of rBCG pMV361-Rho/EGFP induced humoral immune response. The colonization of rBCG in liver, spleen, lung, kidney and caecum was observed by laser confocal microscopy. Real-time quantitative RT-PCR showed a rise expression level of rhomboid protein on the 7th day and a peak on the 14th day and disappearance on the 28th day after immunization. These results have significant implications for the development of rBCG vaccines against avian coccidiosis.
Wang
QiuYue
Q
College of Animal Science and Veterinary Medicine, Jilin University, Changchun, 130062, China.
Li
JianHua
J
Zhang
XiChen
X
Liu
ChengWu
C
Cao
LiLi
L
Ren
KeYan
K
Gong
PengTao
P
Cai
YaNan
Y
D000818:Animals; D002645:Chickens / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D003048:Coccidiosis / Q000276:immunology / Q000517:prevention & control / Q000662:veterinary*; D016786:Eimeria tenella / Q000235:genetics / Q000276:immunology*; D004926:Escherichia coli / Q000235:genetics; D015870:Gene Expression / Q000235:genetics / Q000276:immunology; D005822:Genetic Vectors / Q000235:genetics; D049452:Green Fluorescent Proteins / Q000276:immunology / Q000378:metabolism; D007114:Immunization / Q000379:methods; D008297:Male; D009163:Mycobacterium bovis / Q000235:genetics* / Q000276:immunology* / Q000378:metabolism; D011201:Poultry Diseases / Q000235:genetics / Q000276:immunology / Q000517:prevention & control*; D011897:Random Allocation; D011993:Recombinant Fusion Proteins / Q000276:immunology / Q000378:metabolism; D013047:Specific Pathogen-Free Organisms; D014018:Tissue Distribution
D011993:Recombinant Fusion Proteins; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins