title: Plasma biomarkers in a mouse model of preterm labor.
abstract: Preterm labor (PTL) is frequently associated with inflammation. We hypothesized that biomarkers during pregnancy can identify pregnancies most at risk for development of PTL. An inflammation-induced mouse model of PTL was used. Surface-enhanced laser desorption/ionization time-of-flight mass spectrometry was used to analyze and compare the plasma protein (PP) profile between CD-1 mice injected intrauterine with either lipopolysaccharide (LPS) or PBS on d 14.5 of gestation. The median differences of normalized PP peaks between the two groups were determined using the Mann-Whitney U test and the false discovery rate. In a second series of experiments, both groups of mice were injected with a lower dose of LPS. A total of 1665 peaks were detected. Thirty peaks were highly differentially expressed (p < 0.0001) between the groups. Two 11 kDa protein peaks were identified by MALDI-TOF/TOF-MS and confirmed to be mouse serum amyloid A (SAA) 1 and 2. Plasma SAA2 levels were increased in LPS-treated animals compared with controls and in LPS-treated animals that delivered preterm vs. those that delivered at term. SAA2 has the potential to be a plasma biomarker that can identify pregnancies at risk for development of PTL.
authors.affiliation: Department of Pediatrics, Stanford University, Stanford, CA 94305, USA.
authors.forename: Qing
authors.initials: Q
authors.lastname: Yang
authors.forename: John C
authors.initials: JC
authors.lastname: Whitin
authors.forename: Xuefeng Bruce
authors.initials: XB
authors.lastname: Ling
authors.forename: Nihar R
authors.initials: NR
authors.lastname: Nayak
authors.forename: Harvey J
authors.initials: HJ
authors.lastname: Cohen
authors.forename: Joseph
authors.initials: J
authors.lastname: Jin
authors.forename: James
authors.initials: J
authors.lastname: Schilling
authors.forename: Tom To-Sang
authors.initials: TT
authors.lastname: Yu
authors.forename: Ashima
authors.initials: A
authors.lastname: Madan
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000097:blood; D001798:Blood Proteins / Q000032:analysis*; D004195:Disease Models, Animal; D005260:Female; D007249:Inflammation / Q000150:complications*; D008070:Lipopolysaccharides; D051379:Mice; D008817:Mice, Mutant Strains; D007752:Obstetric Labor, Premature / Q000097:blood* / Q000209:etiology; D011247:Pregnancy; D000685:Serum Amyloid A Protein / Q000032:analysis; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D018709:Statistics, Nonparametric
chemical_list: D015415:Biomarkers; D001798:Blood Proteins; D008070:Lipopolysaccharides; D000685:Serum Amyloid A Protein