Effect of cell density on adipogenic differentiation of mesenchymal stem cells.
The effect of cell density on the adipogenic differentiation of human bone marrow-derived mesenchymal stem cells (MSCs) was investigated by using a patterning technique to induce the formation of a cell density gradient on a micropatterned surface. The adipogenic differentiation of MSCs at a density gradient from 5 x 10(3) to 3 x 10(4) cells/cm2 was examined. Lipid vacuoles were observed at all cell densities after 1-3 weeks of culture in adipogenic differentiation medium although the lipid vacuoles were scarce at the low cell density and abundant at the high cell density. Real-time RT-PCR analysis showed that adipogenesis marker genes encoding peroxisome proliferator-activated receptor gamma2 (PPARgamma2), lipoprotein lipase (LPL), and fatty acid binding protein-4 (FABP4) were detected in the MSCs cultured at all cell densities. The results suggest that there was no apparent effect of cell density on the adipogenic differentiation of human MSCs.
Lu
Hongxu
H
Graduate School of Pure and Applied Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8577, Japan.
Guo
Likun
L
Wozniak
Michal J
MJ
Kawazoe
Naoki
N
Tateishi
Tetsuya
T
Zhang
Xingdong
X
Chen
Guoping
G
D050156:Adipogenesis / Q000235:genetics; D001854:Bone Marrow Cells / Q000502:physiology* / Q000648:ultrastructure; D002452:Cell Count; D005819:Genetic Markers; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000502:physiology* / Q000648:ultrastructure; D023822:Tissue Engineering
D005819:Genetic Markers