A strategy to verify the absence of the pgm locus in Yersinia pestis strain candidates for select agent exemption.
Yersinia pestis is a Department of Health and Human Services select agent as defined in federal regulations. Certain attenuated strains of Y. pestis, such as the pgm(-) strain, are exempt from these regulations. Herein we describe a strategy to verify the absence of the pgm locus in Y. pestis strains being considered as candidates for select agent exemption by PCR analysis of virulence-associated genes.
Jenkins
Amy L
AL
Bacteriology Division, U. S. Army Medical Research Institute of Infectious Diseases, Fort Detrick, Frederick, MD 21702, USA.
Worsham
Patricia L
PL
Welkos
Susan L
SL
D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D017931:DNA Primers / Q000235:genetics; D006801:Humans; D009154:Mutation; D010860:Pigments, Biological / Q000235:genetics* / Q000378:metabolism; D010930:Plague / Q000382:microbiology*; D016133:Polymerase Chain Reaction / Q000379:methods*; D014481:United States; D014483:United States Dept. of Health and Human Services / Q000331:legislation & jurisprudence*; D037521:Virulence Factors / Q000235:genetics / Q000378:metabolism; D015010:Yersinia pestis / Q000235:genetics* / Q000378:metabolism
D001426:Bacterial Proteins; D017931:DNA Primers; D010860:Pigments, Biological; D037521:Virulence Factors