title: Cell outer membrane mimetic modification of a cross-linked chitosan surface to improve its hemocompatibility.
abstract: A novel strategy has been developed to improve the hemocompatibility of chitosan surface by cell outer membrane mimetic structure able to reduce protein adsorption and cell adhesion. Phosphorylcholine dichloride was synthesized and grafted onto a glutaraldehyde-cross-linked chitosan (CS-GA) film surface to prepare phosphorylcholine-coated CS-GA film (CS-GA-PC) through a heterogeneous reaction process. The spectroscopic and contact angle characterization show that a cell outer membrane mimetic structure was formed on the cross-linked chitosan surface, and the significantly improved hemocompatibility of the modified surface was shown by a suppression of 94% on platelet adhesion, a suppression of 60-70% for bovine plasma fibrinogen and bovine serum albumin adsorptions. These results demonstrated that this cell outer membrane mimetic surface modification with phosphorylcholine dichloride is a promising strategy to improve the hemocompatibility of chitosan.
authors.affiliation: Key Laboratory of Synthetic and Natural Functional Molecule Chemistry (Ministry of Education), College of Chemistry and Materials Science, Northwest University, 229 Taibai Beilu, Xi'an 710069, PR China.
authors.forename: Peng-bo
authors.initials: PB
authors.lastname: Huangfu
authors.forename: Ming
authors.initials: M
authors.lastname: Gong
authors.forename: Cunfu
authors.initials: C
authors.lastname: Zhang
authors.forename: Shan
authors.initials: S
authors.lastname: Yang
authors.forename: Jiang
authors.initials: J
authors.lastname: Zhao
authors.forename: Yong-kuan
authors.initials: YK
authors.lastname: Gong
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000494:pharmacology*; D040761:Biomimetic Materials / Q000494:pharmacology*; D002417:Cattle; D002462:Cell Membrane / Q000187:drug effects*; D048271:Chitosan / Q000138:chemical synthesis / Q000737:chemistry*; D003432:Cross-Linking Reagents / Q000494:pharmacology*; D005340:Fibrinogen / Q000378:metabolism; D006801:Humans; D010767:Phosphorylcholine / Q000138:chemical synthesis / Q000737:chemistry; D010973:Platelet Adhesiveness / Q000187:drug effects; D012710:Serum Albumin, Bovine / Q000378:metabolism; D013057:Spectrum Analysis; D013499:Surface Properties / Q000187:drug effects
chemical_list: D001672:Biocompatible Materials; D003432:Cross-Linking Reagents; D010767:Phosphorylcholine; D012710:Serum Albumin, Bovine; D005340:Fibrinogen; D048271:Chitosan