title: Differential proteomic analysis of subfractioned human hepatocellular carcinoma tissues.
abstract: To discover new potential biomarkers of HCC, we used 2-DE gel separation and MALDI-TOF-MS analysis of partially enriched nuclear fractions from liver biopsies of 20 different patients. We obtained a proteomic map of subfractioned liver samples including about 200 common protein spots, among which identified components corresponded to expression products of 52 different genes. A differential analysis of proteins from tumoral and control tissues revealed a significant change in the expression level of 16 proteins associated to cytoskeletal, stress response and metabolic functions. These data may provide novel candidate biomarkers for HCC and useful insights for understanding the mechanisms of HCC pathogenesis and progression.
authors.affiliation: Department of Biomedical Sciences and Technologies, University of Udine, 33100 Udine, Italy.
authors.forename: Erika
authors.initials: E
authors.lastname: Codarin
authors.forename: Giovanni
authors.initials: G
authors.lastname: Renzone
authors.forename: Alessandra
authors.initials: A
authors.lastname: Poz
authors.forename: Claudio
authors.initials: C
authors.lastname: Avellini
authors.forename: Umberto
authors.initials: U
authors.lastname: Baccarani
authors.forename: Francesco
authors.initials: F
authors.lastname: Lupo
authors.forename: Vittorio
authors.initials: V
authors.lastname: di Maso
authors.forename: Saveria Lory
authors.initials: SL
authors.lastname: Crocè
authors.forename: Claudio
authors.initials: C
authors.lastname: Tiribelli
authors.forename: Simona
authors.initials: S
authors.lastname: Arena
authors.forename: Franco
authors.initials: F
authors.lastname: Quadrifoglio
authors.forename: Andrea
authors.initials: A
authors.lastname: Scaloni
authors.forename: Gianluca
authors.initials: G
authors.lastname: Tell