Plant Rho-type (Rop) GTPase-dependent activation of receptor-like cytoplasmic kinases in vitro.
Plants have evolved distinct mechanisms to link Rho-type (Rop) GTPases to downstream signaling pathways as compared to other eukaryotes. Here, experimental data are provided that members of the Medicago, as well as Arabidopsis, receptor-like cytoplasmic kinase family (RLCK Class VI) were strongly and specifically activated by GTP-bound Rop GTPases in vitro. Deletion analysis indicated that the residues implicated in the interaction might be distributed on various parts of the kinases. Using a chimaeric Rop GTPase protein, the importance of the Rho-insert region in kinase activation could also be verified. These data strengthen the possibility that RLCKs may serve as Rop GTPase effectors in planta.
Dorjgotov
Dulguun
D
Laboratory of Functional Cell Biology, Institute of Plant Biology, Biological Research Centre, Hungarian Academy of Sciences, Szeged, Hungary.
Jurca
Manuela E
ME
Fodor-Dunai
Csilla
C
Szucs
Attila
A
Otvös
Krisztina
K
Klement
Eva
E
Bíró
Judit
J
Fehér
Attila
A
D017360:Arabidopsis / Q000201:enzymology* / Q000235:genetics; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D004789:Enzyme Activation / Q000502:physiology; D046913:Medicago truncatula / Q000201:enzymology* / Q000235:genetics; D011494:Protein Kinases / Q000235:genetics / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D020741:rho GTP-Binding Proteins / Q000235:genetics / Q000378:metabolism*
D029681:Arabidopsis Proteins; D011993:Recombinant Fusion Proteins; D011494:Protein Kinases; D020741:rho GTP-Binding Proteins