Siglec-1 on monocytes is a potential risk marker for monitoring disease severity in coronary artery disease.
OBJECTIVE
Siglec-1 has long been considered as an important biomarker of the activation of monocyte/macrophage and a type I interferon-specific imprint, but its role in atherosclerosis has not been elucidated.


METHODS
We examined the expression of Siglec-1 by flow cytometry and RT-PCR in 83 CAD patients and 38 healthy controls. In addition, the levels of serum lipids, Gensini score, hs-CRP and homocysteine were determined.


RESULTS
The transcriptional and protein levels of Siglec-1 on monocytes in CAD patients were significantly increased compared with healthy controls [3.17 versus 1.0, P<0.01; (11.5+/-3.9)% versus (1.8+/-2.0)%, P<0.01], but the increased Siglec-1 had no correlation with the level of native serum lipids. Interestingly, the expression of Siglec-1 was positively correlated with Gensini score (r=0.338, P=0.015), hs-CRP (r=0.316, P=0.016) and homocysteine level (r=0.224, P=0.042).


CONCLUSIONS
Siglec-1 may be considered as a potential non-invasive indicator for monitoring disease severity and a biomarker for predicting the relative risk of cardiovascular events.
Xiong
Yi-song
YS
Department of Laboratory Medicine, Changzheng Hospital, Second Military Medical University and Clinical Immunology Center of PLA, 415 Feng Yang Road, Huang Pu District, Shanghai 200003, People's Republic of China.
Zhou
Yun-heng
YH
Rong
Guang-hua
GH
Wu
Wei-lin
WL
Liang
Yan
Y
Yang
Zai-xing
ZX
Geng
Hong-lian
HL
Zhong
Ren-qian
RQ
D015415:Biomarkers / Q000378:metabolism; D002097:C-Reactive Protein / Q000032:analysis; D003324:Coronary Artery Disease / Q000097:blood / Q000235:genetics / Q000378:metabolism* / Q000473:pathology*; D004587:Electrophoresis, Agar Gel; D005434:Flow Cytometry; D005786:Gene Expression Regulation; D006710:Homocysteine / Q000097:blood; D006801:Humans; D008562:Membrane Glycoproteins / Q000378:metabolism*; D009000:Monocytes / Q000378:metabolism*; D012313:RNA / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011971:Receptors, Immunologic / Q000378:metabolism*; D012307:Risk Factors; D063267:Sialic Acid Binding Ig-like Lectin 1
D015415:Biomarkers; D008562:Membrane Glycoproteins; D012333:RNA, Messenger; D011971:Receptors, Immunologic; C571166:SIGLEC1 protein, human; D063267:Sialic Acid Binding Ig-like Lectin 1; D006710:Homocysteine; D012313:RNA; D002097:C-Reactive Protein