title: The hepatic circadian clock is preserved in a lipid-induced mouse model of non-alcoholic steatohepatitis.
abstract: Recent studies have correlated metabolic diseases, such as metabolic syndrome and non-alcoholic fatty liver disease, with the circadian clock. However, whether such metabolic changes per se affect the circadian clock remains controversial. To address this, we investigated the daily mRNA expression profiles of clock genes in the liver of a dietary mouse model of non-alcoholic steatohepatitis (NASH) using a custom-made, high-precision DNA chip. C57BL/6J mice fed an atherogenic diet for 5 weeks developed hypercholesterolemia, oxidative stress, and NASH. DNA chip analyses revealed that the atherogenic diet had a great influence on the mRNA expression of a wide range of genes linked to mitochondrial energy production, redox regulation, and carbohydrate and lipid metabolism. However, the rhythmic mRNA expression of the clock genes in the liver remained intact. Most of the circadianly expressed genes also showed 24-h rhythmicity. These findings suggest that the biological clock is protected against such a metabolic derangement as NASH.
authors.affiliation: Department of Disease Control and Homeostasis, Kanazawa University Graduate School of Medical Science, 13-1 Takara-machi, Kanazawa, Ishikawa 920-8641, Japan.
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Ando
authors.forename: Toshinari
authors.initials: T
authors.lastname: Takamura
authors.forename: Naoto
authors.initials: N
authors.lastname: Matsuzawa-Nagata
authors.forename: Kosuke R
authors.initials: KR
authors.lastname: Shima
authors.forename: Seiji
authors.initials: S
authors.lastname: Nakamura
authors.forename: Masafumi
authors.initials: M
authors.lastname: Kumazaki
authors.forename: Seiichiro
authors.initials: S
authors.lastname: Kurita
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Misu
authors.forename: Naoyuki
authors.initials: N
authors.lastname: Togawa
authors.forename: Tatsunobu
authors.initials: T
authors.lastname: Fukushima
authors.forename: Akio
authors.initials: A
authors.lastname: Fujimura
authors.forename: Shuichi
authors.initials: S
authors.lastname: Kaneko
mesh_terms: D000818:Animals; D001683:Biological Clocks / Q000235:genetics; D002940:Circadian Rhythm / Q000235:genetics; D004036:Diet, Atherogenic; D004195:Disease Models, Animal; D005234:Fatty Liver / Q000235:genetics / Q000503:physiopathology*; D020869:Gene Expression Profiling; D008099:Liver / Q000378:metabolism / Q000503:physiopathology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D020411:Oligonucleotide Array Sequence Analysis