Cultivation of the Pine Wilt Nematode, Bursaphelenchus xylophilus, in Axenic Culture Media.
The pine wilt nematode, Bursaphelenchus xylophilus, has been cultured axenically in vitro on soy peptone/yeast extract or modified Caenorhabditis medium supplemented with cholesterol and hemoglobin. Although growth, development and reproduction were best in soy peptone/yeast extract medium, satisfactory population size increases were observed in the chemically defined Caenorhaditis medium.
Bolla
R I
RI
Jordan
W
W