Effect of L-arginine on metabolism of polyamines in rat's brain with extrahepatic cholestasis.
Cholestatic encephalopathy results from accumulation of unconjugated bilirubin and hydrophobic bile acids in the brain. The aim of this study was to determine disturbances of polyamine metabolism in the brains of rats with experimental extrahepatic cholestasis and the effects of L-arginine administration. Wister rats were divided into groups: I: sham-operated, II: rats treated with L-arginine, III: animals with bile-duct ligation (BDL), and IV: cholestatic-BDL rats treated with L-arginine. Increased plasma gamma-glutamyltransferase and alkaline phosphatase activity and increased bile-acids and bilirubin levels in BDL rats were reduced by administration of L-arginine (P < 0.001). Cholestasis increased the brain's putrescine (P < 0.001) and decreased spermidine and spermine concentration (P < 0.05). The activity of polyamine oxidase was increased (P < 0.001) and diamine oxidase was decreased (P < 0.001) in the brains of BDL rats. Cholestasis increased the activity of arginase (P < 0.05) and decreased the level of citrulline (P < 0.001). Administration of L-arginine in BDL rats prevents metabolic disorders of polyamines and establishes a neuroprotective role in the brain during cholestasis.
Sokolovic
Dusan
D
Institute of Biochemistry, Medical Faculty in Nis, , Nis, Serbia. soko@medfak.ni.ac.yu
Bjelakovic
Gordana
G
Nikolic
Jelenka
J
Djindjic
Boris
B
Pavlovic
Dusica
D
Kocic
Gordana
G
Stojanovic
Ivana
I
Pavlovic
Voja
V
D000469:Alkaline Phosphatase / Q000097:blood; D000818:Animals; D001120:Arginine / Q000008:administration & dosage* / Q000378:metabolism; D001663:Bilirubin / Q000097:blood; D001921:Brain / Q000187:drug effects / Q000378:metabolism*; D001651:Cholestasis, Extrahepatic / Q000188:drug therapy* / Q000201:enzymology / Q000378:metabolism; D004195:Disease Models, Animal; D008297:Male; D000587:Oxidoreductases Acting on CH-NH Group Donors / Q000378:metabolism; D011073:Polyamines / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D005723:gamma-Glutamyltransferase / Q000097:blood; D000097667:Polyamine Oxidase
D000469:Alkaline Phosphatase; D001120:Arginine; D001663:Bilirubin; D000587:Oxidoreductases Acting on CH-NH Group Donors; D011073:Polyamines; D005723:gamma-Glutamyltransferase; D000097667:Polyamine Oxidase