Mechanical strain using 2D and 3D bioreactors induces osteogenesis: implications for bone tissue engineering.
Fracture healing is a complicated process involving many growth factors, cells, and physical forces. In cases, where natural healing is not able, efforts have to be undertaken to improve healing. For this purpose, tissue engineering may be an option. In order to stimulate cells to form a bone tissue several factors are needed: cells, scaffold, and growth factors. Stem cells derived from bone marrow or adipose tissues are the most useful in this regard. The differentiation of the cells can be accelerated using mechanical stimulation. The first part of this chapter describes the influence of longitudinal strain application. The second part uses a sophisticated approach with stem cells on a newly developed biomaterial (Sponceram) in a rotating bed bioreactor with the administration of bone morphogenetic protein-2. It is shown that such an approach is able to produce bone tissue constructs. This may lead to production of larger constructs that can be used in clinical applications.
van Griensven
M
M
Ludwig Boltzmann Institute for Experimental and Clinical Traumatology, Donaueschingenstrabetae 13, Vienna, A-1200, Austria, Martijn.van.Griensven@lbitrauma.org.
Diederichs
S
S
Roeker
S
S
Boehm
S
S
Peterbauer
A
A
Wolbank
S
S
Riechers
D
D
Stahl
F
F
Kasper
C
C
D001696:Biomechanical Phenomena; D019149:Bioreactors; D001854:Bone Marrow Cells / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D055396:Bone Morphogenetic Protein 2 / Q000378:metabolism / Q000494:pharmacology*; D001842:Bone and Bones / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D002454:Cell Differentiation; D004867:Equipment Design; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins / Q000378:metabolism / Q000494:pharmacology*; D040542:Mechanotransduction, Cellular; D010012:Osteogenesis; D013234:Stem Cells / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D046509:Tissue Culture Techniques / Q000295:instrumentation; D023822:Tissue Engineering / Q000295:instrumentation* / Q000379:methods; D054457:Tissue Scaffolds
C525718:BMP2 protein, human; D055396:Bone Morphogenetic Protein 2; D036341:Intercellular Signaling Peptides and Proteins