title: Antiproliferation effect and mechanism of prostate cancer cell lines as affected by isoflavones from soybean cake.
abstract: The objectives of this study were to determine the antiproliferation effect of prostate cancer cell lines LNCaP and PC-3 as affected by 4 isoflavone fractions prepared from soybean cake and isoflavone standards genistein and daidzein. With the MTT test, most treatments were effective in inhibiting prostate cancer cell growth at a low dose of 5 and 10 mug/mL. In cell cycle analysis, the fractions of aglycon, a mixture of acetylglucoside and aglycon, as well as genistein and a combination of genistein and daidzein standards exhibited a high G2/M ratio for LNCaP, as did the acetylglucoside, genistein and a combination of genistein and daidzein standards for PC-3. Results of Western blotting revealed an increase in p53 protein expression of LNCaP following treatments of the aglycon fraction, genistein and a combination of genistein and daidzein standards. However, all the treatments did not affect Bcl-2 protein expression significantly in both LNCaP and PC-3 cells. A decline in cyclin B1 expression of LNCaP was observed for all the treatments, with the mixture of acetylglucoside and aglycon possessing the most pronounced effect. But for PC-3, a decrease in cyclin B1 expression was shown for all the isoflavones, with the exception of malonylglucoside, glucoside and acetylglucoside fractions. The outcome of this study may provide a basis for possible production of functional food in the future with soybean cake as raw material.
authors.affiliation: Department of Urology, Changhua Christian Hospital, Changhua, Taiwan.
authors.forename: Bai Fu
authors.initials: BF
authors.lastname: Wang
authors.forename: Jinn Shyan
authors.initials: JS
authors.lastname: Wang
authors.forename: Jyh Feng
authors.initials: JF
authors.lastname: Lu
authors.forename: Tsai Hua
authors.initials: TH
authors.lastname: Kao
authors.forename: Bing Huei
authors.initials: BH
authors.lastname: Chen
mesh_terms: D000975:Antioxidants / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects / Q000235:genetics; D002453:Cell Cycle / Q000187:drug effects / Q000235:genetics; D002455:Cell Division / Q000187:drug effects*; D045744:Cell Line, Tumor; D015870:Gene Expression / Q000187:drug effects; D016158:Genes, p53 / Q000235:genetics; D006801:Humans; D007529:Isoflavones / Q000032:analysis / Q000494:pharmacology*; D008297:Male; D011471:Prostatic Neoplasms / Q000473:pathology*; D013025:Glycine max / Q000737:chemistry*
chemical_list: D000975:Antioxidants; D007529:Isoflavones