title: The proteome of mesenteric lymph during acute pancreatitis and implications for treatment.
abstract: BACKGROUND
  The protein fraction of mesenteric lymph during acute pancreatitis and other critical illness is thought to contain toxic factors. However, we do not have a complete description of the mesenteric lymph proteome during acute pancreatitis.
  
  
  OBJECTIVE
  The aim of this study was to define the proteomic changes in mesenteric lymph during acute pancreatitis.
  
  
  METHODS
  Animal Laboratory, University of Auckland, New Zealand.
  
  
  METHODS
  Mesenteric lymph was collected from sixteen male Wistar rats randomised to Group 1 (n=8) with taurocholate induced acute pancreatitis and Group 2 (n=8) sham control. The lymph was subjected to proteomic analysis using iTRAQ (Applied Biosystems, Foster City, CA, USA) and liquid chromatography-tandem mass spectrometry.
  
  
  RESULTS
  Two hundred and forty-five proteins including 35 hypothetical proteins were identified in mesenteric lymph. Eight of the 245 proteins had a significant increase in their relative abundance in acute pancreatitis conditioned mesenteric lymph, and 7 of these were pancreatic catabolic enzymes (pancreatic amylase 2, pancreatic lipase, carboxypeptidase A2, chymotrypsinogen B, carboxypeptidase B1, cationic trypsinogen, ribonuclease 1).
  
  
  CONCLUSIONS
  This is the first comprehensive description of the proteome of mesenteric lymph during acute pancreatitis and has demonstrated a significantly increased relative abundance of 7 secreted pancreatic catabolic enzymes in acute pancreatitis conditioned mesenteric lymph. This study provides a clear rationale for further research to investigate the efficacy of enteral protease inhibitors in the treatment of acute pancreatitis.
authors.affiliation: Department of Surgery, Faculty of Medicine and Health Sciences, University of Auckland, Auckland, New Zealand.
authors.forename: Anubhav
authors.initials: A
authors.lastname: Mittal
authors.forename: Anthony R J
authors.initials: AR
authors.lastname: Phillips
authors.forename: Martin
authors.initials: M
authors.lastname: Middleditch
authors.forename: Katya
authors.initials: K
authors.lastname: Ruggiero
authors.forename: Benjamin
authors.initials: B
authors.lastname: Loveday
authors.forename: Brett
authors.initials: B
authors.lastname: Delahunt
authors.forename: Garth J S
authors.initials: GJ
authors.lastname: Cooper
authors.forename: John A
authors.initials: JA
authors.lastname: Windsor
mesh_terms: D000208:Acute Disease; D000818:Animals; D002853:Chromatography, Liquid / Q000379:methods; D030562:Databases, Protein; D008196:Lymph / Q000378:metabolism*; D008297:Male; D008643:Mesentery; D010195:Pancreatitis / Q000139:chemically induced / Q000378:metabolism* / Q000628:therapy; D020543:Proteome / Q000032:analysis*; D040901:Proteomics / Q000379:methods*; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D015203:Reproducibility of Results; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D013656:Taurocholic Acid
chemical_list: D020543:Proteome; D013656:Taurocholic Acid