[Characteristics of human lipoaspirate-isolated mesenchymal stromal cells cultivated under a lower oxygen tension].
In the present study we investigated the effect of low oxygen concentration in the cultivation medium on proliferation, viability and immunophenotype of human mesenchymal stromal cells isolated from lipoaspirate (lMSC). It was shown that proliferation activity of the cells under hypoxic conditions was, on average, 2.9 times higher compared to that under commonly accepted normoxic conditions. Redused oxygen level in the culture medium did not cause any change in IMSC viability and immunophenotype. Thus, permanent culture of lMSC in the medium with a lower oxygen tension can prove an efficient approach to obtain a higher mass of cells which maintain their characteristics in a shorter period of time, which can be of demand for regenerative medicine.
Buravkova
L B
LB
Grinakovskaia
O S
OS
Andreeva
E P
EP
Zhambalova
A P
AP
Kozionova
M P
MP
D000273:Adipose Tissue / Q000166:cytology* / Q000502:physiology; D015703:Antigens, CD / Q000276:immunology; D018929:Cell Culture Techniques; D015687:Cell Hypoxia / Q000187:drug effects / Q000502:physiology; D049109:Cell Proliferation / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D006801:Humans; D016130:Immunophenotyping; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000276:immunology / Q000502:physiology; D010100:Oxygen / Q000494:pharmacology*; D017154:Stromal Cells / Q000166:cytology* / Q000276:immunology / Q000502:physiology
D015703:Antigens, CD; D010100:Oxygen