Preparation of immunogens and production of antibodies.
The quality of reagents greatly affects the interpretation of serological tests. Methods used in conventional viral purification and molecular cloning and expression of target viral proteins to obtain antigens for immunization are presented. Immunization of rabbits, mice and chickens and isolation of immunoglobulin from immunized animals also are described.
Hsu
Hei-Ti
HT
Floral and Nursery Plants Research Unit, U.S. National Arboretum, USDA-ARS, Beltsville, MD 20705, USA.
Chen
Tsung-Chi
TC
Chang
Chin-An
CA
Yeh
Shyi-Dong
SD
D000818:Animals; D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D000917:Antibody Formation / Q000276:immunology* / Q000502:physiology; D000918:Antibody Specificity / Q000502:physiology*; D000941:Antigens / Q000276:immunology / Q000302:isolation & purification*; D019411:Clinical Laboratory Techniques; D007114:Immunization / Q000379:methods*; D051379:Mice; D011817:Rabbits; D012680:Sensitivity and Specificity; D014614:Vaccines, Synthetic / Q000276:immunology
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D000941:Antigens; D014614:Vaccines, Synthetic