title: Cyclin D1 negatively regulates the expression of differentiation genes in HT-29 M6 mucus-secreting colon cancer cells.
abstract: HT-29 M6 colon cancer cells differentiate to a mucus-secreting phenotype in culture. We found that the pattern of cyclin D1 expression in HT-29 M6 cells did not correlate with instances of cell proliferation but was specifically induced during a dedifferentiation process following disaggregation of epithelial cell layers, even under conditions that did not allow cell cycle reentrance. Interestingly, ectopic expression of cyclin D1 in differentiated cells led to the inhibition of the transcriptional activity of differentiation gene promoters, such as the mucin MUC1. We thus propose that the overexpression of cyclin D1 found in colon cancer favours tumour dedifferentiation as one mechanism of tumour progression.
authors.affiliation: IMIM - Hospital del Mar, Barcelona, Spain.
authors.forename: Clara
authors.initials: C
authors.lastname: Mayo
authors.forename: Xavier
authors.initials: X
authors.lastname: Mayol
mesh_terms: D015153:Blotting, Western; D002453:Cell Cycle / Q000502:physiology; D054337:Cell Dedifferentiation / Q000235:genetics*; D002454:Cell Differentiation / Q000235:genetics; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D003110:Colonic Neoplasms / Q000235:genetics* / Q000378:metabolism; D019938:Cyclin D1 / Q000235:genetics* / Q000378:metabolism; D018450:Disease Progression; D005434:Flow Cytometry; D015870:Gene Expression; D015972:Gene Expression Regulation, Neoplastic / Q000502:physiology*; D006801:Humans; D018396:Mucin-1 / Q000235:genetics; D009093:Mucus / Q000378:metabolism*; D011401:Promoter Regions, Genetic; D014162:Transfection
chemical_list: D018396:Mucin-1; D019938:Cyclin D1