Isolation, structure elucidation and antibacterial activities of streptothricin acids.
Five streptothricin acids (compounds 1-5) were isolated by ion-exchange resin chromatography and preparative RP-HPLC from the fermentation broth of Streptomyces qinlingensis. Their structures were elucidated mainly by analyses of the IR, HR-EIS-MS and NMR spectral data. They were deduced as two known compounds, streptothricin F acid (1) and streptothricin D acid (2), and three new compounds, 12-carbamoylstreptothricin E acid (3), 12-carbamoylstreptothricin D acid (4) and N-acetyl-streptothricin D acid (5), respectively. The antibacterial activities of 1-5 against Escherichia coli, Bacillus subtilis, Staphylococcus aureus, Bacillus cereus and Pseudomonas aeruginosa were assayed by micro-broth dilution. Comparison of the MICs with streptothricin F and D showed that the antimicrobial activities of 1-5 were decreased significantly but varied with the structures.
Ji
Zhiqin
Z
Institute of Pesticide Science, Northwest Agricultural and Forestry University, Shaanxi, China.
Wang
Mingan
M
Wei
Shaopeng
S
Zhang
Jiwen
J
Wu
Wenjun
W
D000143:Acids / Q000737:chemistry; D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000494:pharmacology*; D001419:Bacteria / Q000187:drug effects*; D002851:Chromatography, High Pressure Liquid; D005285:Fermentation; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D008826:Microbial Sensitivity Tests; D021241:Spectrometry, Mass, Electrospray Ionization; D013055:Spectrophotometry, Infrared; D013309:Streptothricins / Q000737:chemistry* / Q000494:pharmacology*
D000143:Acids; D000900:Anti-Bacterial Agents; D013309:Streptothricins