title: Evidence Against Amplification of Four Genes in Giant Cells Induced by Meloidogyne incognita.
abstract: Giant-cell DNA was isolated from pea (Pisum sativum) inoculated with Meloidogyne incognita and used in slot blots to test for selective sequence amplification. Four sequences representing low (ribulose 1,5-bisphosphate carboxylase and actin), mid-level (histone 3), and highly repetitive (large ribosomal repeat) sequence DNA were used as probes. Known amounts of root-tip DNA and giant-cell DNA were blotted onto hybridization membranes and probed. The signal strength on autoradiographs containing equal amounts of root-tip DNA and giant-cell DNA were compared with a scanning densitometer. No difference in signal strength between equal amounts of root-tip DNA and giant-cell DNA was found. Thus, for the probes tested, there is no difference in copy number and, hence, no selective DNA sequence amplification has occurred.
authors.forename: R J
authors.initials: RJ
authors.lastname: Wiggers
authors.forename: C W
authors.initials: CW
authors.lastname: Magill
authors.forename: J L
authors.initials: JL
authors.lastname: Starr
authors.forename: H J
authors.initials: HJ
authors.lastname: Price