title: High-throughput shotgun lipidomics by quadrupole time-of-flight mass spectrometry.
abstract: Technological advances in mass spectrometry and meticulous method development have produced several shotgun lipidomic approaches capable of characterizing lipid species by direct analysis of total lipid extracts. Shotgun lipidomics by hybrid quadrupole time-of-flight mass spectrometry allows the absolute quantification of hundreds of molecular glycerophospholipid species, glycerolipid species, sphingolipid species and sterol lipids. Future applications in clinical cohort studies demand detailed lipid molecule information and the application of high-throughput lipidomics platforms. In this review we describe a novel high-throughput shotgun lipidomic platform based on 96-well robot-assisted lipid extraction, automated sample infusion by mircofluidic-based nanoelectrospray ionization, and quantitative multiple precursor ion scanning analysis on a quadrupole time-of-flight mass spectrometer. Using this platform to compile comprehensive lipid arrays associated with metabolic dysfunctions is a powerful strategy for pinpointing the mechanistic details by which alterations in tissue-specific lipid metabolism are directly linked to the etiology of many lipid-mediated disorders.
authors.affiliation: Sahlgrenska Center for Cardiovascular and Metabolic Research/Wallenberg Laboratory, Göteborg University, Göteborg, Sweden.
authors.forename: Marcus
authors.initials: M
authors.lastname: Ståhlman
authors.forename: Christer S
authors.initials: CS
authors.lastname: Ejsing
authors.forename: Kirill
authors.initials: K
authors.lastname: Tarasov
authors.forename: Jeanna
authors.initials: J
authors.lastname: Perman
authors.forename: Jan
authors.initials: J
authors.lastname: Borén
authors.forename: Kim
authors.initials: K
authors.lastname: Ekroos
mesh_terms: D000818:Animals; D019295:Computational Biology / Q000379:methods*; D006801:Humans; D008052:Lipid Metabolism, Inborn Errors / Q000378:metabolism; D008055:Lipids / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
chemical_list: D008055:Lipids