title: Direct detection of plant viruses in potato tubers using real-time PCR.
abstract: Virus indexing of seed potatoes can be carried out by growing eye plugs to produce small plants and then testing them by ELISA, but this method is time consuming. Direct testing of the eye plugs by ELISA is not reliable, and so a method has been developed for the routine testing of seed potatoes for virus by PCR.
authors.affiliation: Central Science Laboratories, Sand Hutton, York, UK.
authors.forename: Neil
authors.initials: N
authors.lastname: Boonham
authors.forename: Lynn
authors.initials: L
authors.lastname: Laurenson
authors.forename: Rebecca
authors.initials: R
authors.lastname: Weekes
authors.forename: Rick
authors.initials: R
authors.lastname: Mumford
mesh_terms: D019411:Clinical Laboratory Techniques; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D021141:Nucleic Acid Amplification Techniques; D010942:Plant Viruses / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012367:RNA, Viral / Q000032:analysis / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012680:Sensitivity and Specificity; D011198:Solanum tuberosum / Q000821:virology*
chemical_list: D012367:RNA, Viral