Curcumin suppresses PPARdelta expression and related genes in HT-29 cells.
OBJECTIVE
To investigate the effects of curcumin on the expression of peroxisome proliferator-activated receptordelta (PPARdelta) and related genes in HT-29 cells.


METHODS
HT-29 cells were treated with curcumin (0-80 micromol/L) for 24 h. The effects of curcumin on the morphology of HT-29 cells were studied by Hoechst 33342 staining. The activity of caspase-3 was determined using DEVD-pNA as substrate. The levels of peroxisome PPARdelta, 14-3-3epsilon and vascular endothelial growth factor (VEGF) in HT-29 cells were determined by Western blotting analysis and their mRNA expression was determined by real-time quantitative RT-PCR.


RESULTS
Treatment with 10-80 micromol/L curcumin induced typical features of apoptosis and activated the caspase-3 in HT-29 cells. The expression of PPARdelta, 14-3-3epsilon and VEGF was reduced and the activity of beta-catenin/Tcf-4 signaling was inhibited by curcumin treatment.


CONCLUSIONS
Curcumin can induce apoptosis of HT-29 cells and down-regulate the expression of PPARdelta, 14-3-3epsilon and VEGF in HT-29.
Wang
Jin-Bo
JB
Department of Chemistry, College of Science, Zhejiang University, Hanzhou 310027, Zhejiang Province, China.
Qi
Li-Li
LL
Zheng
Shui-Di
SD
Wang
Heng-Zheng
HZ
Wu
Tian-Xing
TX
D048948:14-3-3 Proteins / Q000187:drug effects / Q000235:genetics; D017209:Apoptosis / Q000187:drug effects; D053148:Caspase 3 / Q000187:drug effects / Q000378:metabolism; D045744:Cell Line, Tumor; D003110:Colonic Neoplasms / Q000235:genetics* / Q000473:pathology; D003474:Curcumin / Q000494:pharmacology*; D015536:Down-Regulation / Q000187:drug effects; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D006801:Humans; D047494:PPAR delta / Q000187:drug effects / Q000235:genetics*; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics
D048948:14-3-3 Proteins; D047494:PPAR delta; D042461:Vascular Endothelial Growth Factor A; D053148:Caspase 3; D003474:Curcumin