title: Heterotypic interactions enabled by polarized neutrophil microdomains mediate thromboinflammatory injury.
abstract: Selectins and their ligands mediate leukocyte rolling, allowing interactions with chemokines that lead to integrin activation and arrest. Here we show that E-selectin is crucial for generating a secondary wave of activating signals, transduced specifically by E-selectin ligand-1, that induces polarized, activated alpha(M)beta(2) integrin clusters at the leading edge of crawling neutrophils, allowing capture of circulating erythrocytes or platelets. In a humanized mouse model of sickle cell disease, the capture of erythrocytes by alpha(M)beta(2) microdomains leads to acute lethal vascular occlusions. In a model of transfusion-related acute lung injury, polarized neutrophils capture circulating platelets, resulting in the generation of oxidative species that produce vascular damage and lung injury. Inactivation of E-selectin or alpha(M)beta(2) prevents tissue injury in both inflammatory models, suggesting broad implications of this paradigm in thromboinflammatory diseases. These results indicate that endothelial selectins can influence neutrophil behavior beyond its canonical rolling step through delayed, organ-damaging, polarized activation.
authors.affiliation: Department of Medicine, Mount Sinai School of Medicine, New York, New York, USA.
authors.forename: Andrés
authors.initials: A
authors.lastname: Hidalgo
authors.forename: Jungshan
authors.initials: J
authors.lastname: Chang
authors.forename: Jung-Eun
authors.initials: JE
authors.lastname: Jang
authors.forename: Anna J
authors.initials: AJ
authors.lastname: Peired
authors.forename: Elaine Y
authors.initials: EY
authors.lastname: Chiang
authors.forename: Paul S
authors.initials: PS
authors.lastname: Frenette
mesh_terms: D000818:Animals; D001792:Blood Platelets / Q000502:physiology; D019040:E-Selectin / Q000502:physiology*; D004912:Erythrocytes / Q000502:physiology; D007249:Inflammation / Q000503:physiopathology* / Q000517:prevention & control*; D019041:L-Selectin / Q000235:genetics / Q000502:physiology; D007962:Leukocytes / Q000187:drug effects / Q000502:physiology*; D016177:Macrophage-1 Antigen / Q000502:physiology; D021962:Membrane Microdomains / Q000502:physiology*; D051379:Mice; D008856:Microscopy, Fluorescence; D009504:Neutrophils / Q000187:drug effects / Q000502:physiology*; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology; D014947:Wounds and Injuries / Q000503:physiopathology
chemical_list: D019040:E-Selectin; D016177:Macrophage-1 Antigen; D014409:Tumor Necrosis Factor-alpha; D019041:L-Selectin