Study on the antiendotoxin action of Pulsatillae Decoction using an Affymetrix rat genome array.
A high-throughput and efficient Affymetrix rat genome array was used to investigate the pharmacological mechanism of the traditional Chinese medicine, Pulsatillae Decoction (PD), used for the treatment of diseases induced by lipopolysaccharide (LPS). Rat intestinal microvascular endothelial cells (RIMECs) were challenged with 1mug/ml LPS for 3h, and then treated with PD at a concentration of 1mg/ml for 24h. Total RNA from each treatment group was extracted from cultured RIMECs for detection by the Affymetrix Rat Genome 230 2.0 Array. The results showed that 36 genes were upregulated and 33 genes were downregulated in the LPS group vs. the blank control group; 566 genes were upregulated and 12 genes were downregulated in the PD-treated group vs. the LPS group; and 93 genes were upregulated and 29 genes were downregulated in the PD-treated group vs. the blank control group. The analysis of these data suggested that PD specifically and effectively reduce damage induced by LPS, and improved physiological and biochemical responses to counteract the effects of LPS.
Hu
Yiyi
Y
Institue of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, PR China. ylhu@njau.edu.cn
Chen
Xi
X
Lin
Hong
H
Hu
Yuanliang
Y
Mu
Xiang
X
D000818:Animals; D002478:Cells, Cultured; D015536:Down-Regulation; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D042783:Endothelial Cells / Q000187:drug effects* / Q000378:metabolism; D004731:Endotoxins / Q000037:antagonists & inhibitors* / Q000276:immunology; D020869:Gene Expression Profiling; D008070:Lipopolysaccharides / Q000037:antagonists & inhibitors / Q000276:immunology; D020411:Oligonucleotide Array Sequence Analysis; D051381:Rats; D015854:Up-Regulation / Q000187:drug effects*
D004365:Drugs, Chinese Herbal; D004731:Endotoxins; D008070:Lipopolysaccharides; C540801:pulsatillae