A rapid and reliable direct method for quantifying meat acylglycerides with monomode microwave irradiation.
A rapid methodology for direct analysis of meat acylglycerides is proposed. A transesterification is carried out in a microwave reactor consisting of a monomode oven using chlorotrimethylsilane (CTMS) and methanol. High-temperature gas chromatography was used to check the absence of underivatized acylglycerides. Whereas transesterification is complete after 30s at 90 degrees C in the microwave method, the reference method needs 2h to complete this process. Moreover, the CTMS-microwave method shows higher recoveries of individual saturated, monounsaturated and polyunsaturated fatty acids. No influence of microwave irradiation on the composition of the fatty acids was observed.
Tomàs
Albert
A
Chemistry Department, Lleida University, Lleida, Catalonia, Spain.
Tor
Marc
M
Villorbina
Gemma
G
Canela
Ramon
R
Balcells
Mercè
M
Eras
Jordi
J
D000818:Animals; D002849:Chromatography, Gas / Q000379:methods; D003627:Data Interpretation, Statistical; D004951:Esterification; D005989:Glycerides / Q000032:analysis*; D008460:Meat / Q000032:analysis*; D000432:Methanol / Q000737:chemistry; D008872:Microwaves; D013696:Temperature; D014297:Trimethylsilyl Compounds / Q000737:chemistry*
D005989:Glycerides; D014297:Trimethylsilyl Compounds; C039293:trimethylchlorosilane; D000432:Methanol