Structure revision of the lantibiotic 97518.
The lantibiotic 97518, produced by a Planomonospora sp., was reported as a 2194 Da polypeptide comprising 24 amino acid residues with five thioether bridges. It was assigned to the mersacidin subgroup of type B lantibiotics by Castiglione et al. (Biochemistry 2007, 46, 5884-5897) and named planosporicin. New analytical, chemical, and genetic data and reinterpretation of the published NMR chemical shifts enable structure revision of 97518. The resulting revision of the 97518 structure involves both a shift of two amino acids and a reorganization of two thioether bridges. With this revision, the lantibiotic 97518 becomes a clear member of the nisin subgroup of compounds.
Maffioli
Sonia I
SI
NAICONS, New Anti-Infective CONSortium, Milan, Italy. SMaffioli@naicons.com
Potenza
Donatella
D
Vasile
Francesca
F
De Matteo
Marilenia
M
Sosio
Margherita
M
Marsiglia
Barbara
B
Rizzo
Vincenzo
V
Scolastico
Carlo
C
Donadio
Stefano
S
D000192:Actinomycetales / Q000737:chemistry*; D000595:Amino Acid Sequence; D001430:Bacteriocins / Q000737:chemistry*; D008969:Molecular Sequence Data; D015394:Molecular Structure; D019906:Nuclear Magnetic Resonance, Biomolecular; D010455:Peptides / Q000737:chemistry
D001430:Bacteriocins; D010455:Peptides; C520832:planosporicin