The effects of nicotinamide adenine dinucleotide on intracerebral hemorrhage-induced brain injury in mice.
In the present study, we investigated whether the administration of nicotinamide adenine dinucleotide (NAD+) provides brain protection in a mouse model of intracerebral hemorrhage (ICH). Male CD-1 mice were divided into sham, ICH treated with vehicle and ICH treated with NAD+ (10 or 20 mg/kg, intranasal) groups. Intranasal delivery of NAD+ resulted in an increase in NAD+ contents in the brains. ICH was induced by collagenase injection. Neurological function, hemorrhage volume and brain edema were measured 24 hours after injection. ICH caused significant neurological deficit with associated brain edema. NAD+ (10 and 20 mg/kg) failed to reduce brain injury after ICH. These results suggest that NAD+ has no neuroprotective effect at 24 hours after ICH.
Tsuchiyama
Reiko
R
Department of Physiology and Pharmacology, Loma Linda University, Loma Linda, CA 92354, USA.
Sozen
Takumi
T
Manaenko
Anatol
A
Zhang
John H
JH
Tang
Jiping
J
D000281:Administration, Intranasal; D000704:Analysis of Variance; D000818:Animals; D001929:Brain Edema / Q000188:drug therapy / Q000209:etiology; D001930:Brain Injuries / Q000188:drug therapy* / Q000209:etiology* / Q000378:metabolism; D002543:Cerebral Hemorrhage / Q000139:chemically induced / Q000150:complications*; D017364:Collagenases; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D008297:Male; D051379:Mice; D009243:NAD / Q000008:administration & dosage* / Q000378:metabolism; D009460:Neurologic Examination / Q000379:methods; D018696:Neuroprotective Agents / Q000008:administration & dosage*
D018696:Neuroprotective Agents; D009243:NAD; D017364:Collagenases