title: Development of a method for the determination of vardenafil in human plasma by high performance liquid chromatography with UV detection.
abstract: A simple high-performance liquid chromatographic (HPLC) method with photometric detection is described for the determination of vardenafil hydrochloride, a phosphodiesterase V inhibitor, in human plasma. Chromatographic separation of the analyte and internal standard was achieved on an analytical 250 x 4.6 mm i.d. reversed-phase Kromasil KR 100 C(18) (5 microm particle size) column using a mobile phase of acetonitrile-potassium dihydrogen phosphate (30:70 v/v). The run time was less than 15 min. Column eluate was monitored at 230 nm. The linearity over the concentration range of 10-1500 ng/mL for vardenafil was obtained and the limit of quantification (LOQ) was 10 ng/mL. The method has been applied to analysis of the vardenafil concentrations for application in pharmacokinetic studies.
authors.affiliation: Dipartimento di Scienze del Farmaco, Facoltà di Farmacia, Università degli Studi G. D'Annunzio, Chieti-Pescara, Chieti, Italy. g.carlucci@unich.it
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Carlucci
authors.forename: Paola
authors.initials: P
authors.lastname: Palumbo
authors.forename: Piera
authors.initials: P
authors.lastname: Iuliani
authors.forename: Giancarlo
authors.initials: G
authors.lastname: Palumbo
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D007093:Imidazoles / Q000097:blood*; D016018:Least-Squares Analysis; D010726:Phosphodiesterase Inhibitors / Q000097:blood*; D010879:Piperazines / Q000097:blood*; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet / Q000379:methods*; D013450:Sulfones / Q000097:blood; D014227:Triazines / Q000097:blood; D000069058:Vardenafil Dihydrochloride
chemical_list: D007093:Imidazoles; D010726:Phosphodiesterase Inhibitors; D010879:Piperazines; D013450:Sulfones; D014227:Triazines; D000069058:Vardenafil Dihydrochloride