Sequential assembly of centromeric proteins in male mouse meiosis.
The assembly of the mitotic centromere has been extensively studied in recent years, revealing the sequence and regulation of protein loading to this chromosome domain. However, few studies have analyzed centromere assembly during mammalian meiosis. This study specifically targets this approach on mouse spermatocytes. We have found that during prophase I, the proteins of the chromosomal passenger complex Borealin, INCENP, and Aurora-B load sequentially to the inner centromere before Shugoshin 2 and MCAK. The last proteins to be assembled are the outer kinetochore proteins BubR1 and CENP-E. All these proteins are not detected at the centromere during anaphase/telophase I and are then reloaded during interkinesis. The loading sequence of the analyzed proteins is similar during prophase I and interkinesis. These findings demonstrate that the interkinesis stage, regularly overlooked, is essential for centromere and kinetochore maturation and reorganization previous to the second meiotic division. We also demonstrate that Shugoshin 2 is necessary for the loading of MCAK at the inner centromere, but is dispensable for the loading of the outer kinetochore proteins BubR1 and CENP-E.
Parra
María Teresa
MT
Departamento de Biología, Unidad de Biología Celular, Facultad de Ciencias, Universidad Autónoma de Madrid, Madrid, Spain. mayte.parra@uam.es
Gómez
Rocío
R
Viera
Alberto
A
Llano
Elena
E
Pendás
Alberto M
AM
Rufas
Julio S
JS
Suja
José A
JA
D000818:Animals; D018797:Cell Cycle Proteins / Q000378:metabolism*; D002503:Centromere / Q000737:chemistry* / Q000378:metabolism; D002868:Chromosomal Proteins, Non-Histone / Q000378:metabolism*; D016547:Kinesins / Q000378:metabolism*; D018386:Kinetochores / Q000737:chemistry / Q000378:metabolism; D008297:Male; D008540:Meiosis; D049469:Meiotic Prophase I; D051379:Mice; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism*; D013090:Spermatocytes / Q000648:ultrastructure
C584278:Bub1b protein, mouse; D018797:Cell Cycle Proteins; D002868:Chromosomal Proteins, Non-Histone; C532340:SGOL2 protein, mouse; C068688:centromere protein E; C090464:Bub1 spindle checkpoint protein; D017346:Protein Serine-Threonine Kinases; D016547:Kinesins