Development of enzyme linked, tissue blot and dot blot immunoassays for plant virus detection.
Immunoassays are among the most powerful and useful techniques for analysis of biological materials. There are numerous variations in which immunoassays can be performed. Coupled with enzyme, using chromogenic substrates, the enzyme immunoassay technique is used to trace the target antigen in tissues. The technique also is used to measure the concentration of antigen in tissue extracts. This chapter provides fundamental information that is needed to carry out the routinely used procedures in plant virus research.
Hsu
Hei-Ti
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Floral and Nursery Plants Research Unit, U.S. National Arboretum, USDA-ARS, Beltsville, MD 20705, USA.
D019411:Clinical Laboratory Techniques; D007118:Immunoassay; D015151:Immunoblotting / Q000379:methods; D007124:Immunoenzyme Techniques / Q000379:methods*; D010942:Plant Viruses / Q000276:immunology / Q000302:isolation & purification*; D012680:Sensitivity and Specificity