Degradation of endocrine disrupting chemicals by genetic transformants in Irpex lacteus with an inducible laccase gene of Phlebia tremellosa.
Irpex lacteus was genetically transformed using an laccase expression vector to get increased laccase producing strains. Stable integration of the vector was confirmed by PCR using the vector-specific primers, and the transformants showed increased laccase activities. When the transformants were grown with several endocrine disrupting chemicals, laccase activity of each transformant was induced up to six times higher than that of the wild type. They showed increased degrading activities against EDCs as well as increased removal rates of estrogenic activities generated by the EDCs than the wild type, and the laccase expression was increased during the degradations of the EDCs.
Kum
Hyunwoo
H
Department of Biochemistry, Molecular Microbiology Lab, Kangwon National University, Chunchon, 200-701, Korea.
Kim
Myung K
MK
Choi
Hyoung T
HT
D001487:Basidiomycota / Q000235:genetics*; D001673:Biodegradation, Environmental; D052244:Endocrine Disruptors / Q000378:metabolism*; D004790:Enzyme Induction; D004967:Estrogens / Q000378:metabolism; D042845:Laccase / Q000235:genetics*; D020072:Polyporales / Q000235:genetics* / Q000378:metabolism; D014170:Transformation, Genetic
D052244:Endocrine Disruptors; D004967:Estrogens; D042845:Laccase