title: Cultivation of fastidious bacteria by viability staining and micromanipulation in a soil substrate membrane system.
abstract: Soil substrate membrane systems allow for microcultivation of fastidious soil bacteria as mixed microbial communities. We isolated established microcolonies from these membranes by using fluorescence viability staining and micromanipulation. This approach facilitated the recovery of diverse, novel isolates, including the recalcitrant bacterium Leifsonia xyli, a plant pathogen that has never been isolated outside the host.
authors.affiliation: School of Biotechnology and Biomolecular Sciences, University of New South Wales, Randwick, NSW 2052, Australia. b.ferrari@unsw.edu.au
authors.forename: B C
authors.initials: BC
authors.lastname: Ferrari
authors.forename: M R
authors.initials: MR
authors.lastname: Gillings
mesh_terms: D001419:Bacteria / Q000145:classification* / Q000235:genetics / Q000254:growth & development* / Q000302:isolation & purification; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005456:Fluorescent Dyes / Q000494:pharmacology; D050296:Microbial Viability; D008846:Micromanipulation / Q000379:methods; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D012988:Soil Microbiology; D013194:Staining and Labeling / Q000379:methods
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005456:Fluorescent Dyes; D012336:RNA, Ribosomal, 16S