The study of phosphate transporter NAPI2B expression in different histological types of epithelial ovarian cancer.
The identification of markers that are specifically expressed by different histological types of epithelial ovarian cancer (EOC) may lead to the development of novel and more specific diagnostic and therapeutic strategies. Sodium-dependent phosphate transporter NaPi2b (or MX35 ovarian cancer antigen) is a novel perspective marker of EOC. To date, the studies on NaPi2b/MX35 expression in different histological types of EOC are limited.


OBJECTIVE
To examine NaPi2b/MX35 expression in different histological types of epithelial ovarian tumors.


METHODS
Here, we describe the analysis of NaPi2b expression in serous (n = 17), endometrioid (n = 8), and mucinous ovarian tumors (n = 3) by Western-blotting (WB), immunohistochemistry and RT-PCR.


RESULTS
The results of immunohistochemical and WB analysis showed that benign and well-differentiated malignant papillary serous tumors as well as well-differentiated malignant endometriod tumors overexpress NaPi2b protein. However, no overexpression of NaPi2b was detected in benign and malignant mucinous tumors as well as in poorly differentiated endometriod tumors. Notably, the expression NaPi2b mRNA was detected in all investigated histological types of EOC.


CONCLUSIONS
We have shown the differential expression profile of NaPi2b phosphate transporter at protein level in various histological types of epithelial ovarian cancer. This finding might facilitate the development of more effective approaches for diagnosis and treatment of this disease.
Gryshkova
V
V
Department of Cell Signaling, Institute of Molecular Biology and Genetics NAS of Ukraine, Kyiv, 03680, Ukraine.
Goncharuk
I
I
Gurtovyy
V
V
Khozhayenko
Yu
Y
Nespryadko
S
S
Vorobjova
L
L
Usenko
V
V
Gout
I
I
Filonenko
V
V
Kiyamova
R
R
D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000032:analysis / Q000235:genetics*; D018269:Carcinoma, Endometrioid / Q000175:diagnosis / Q000235:genetics*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008875:Middle Aged; D018297:Neoplasms, Cystic, Mucinous, and Serous / Q000175:diagnosis / Q000235:genetics*; D010051:Ovarian Neoplasms / Q000175:diagnosis / Q000235:genetics*; D028061:Phosphate Transport Proteins / Q000032:analysis / Q000235:genetics*; D055815:Young Adult
D014408:Biomarkers, Tumor; D028061:Phosphate Transport Proteins