title: Molecular markers for Phlebotomus papatasi (Diptera: Psychodidae) and their usefulness for population genetic analysis.
abstract: Three molecular typing tools: multilocus microsatellite typing, cytochrome b sequence analysis and internal transcribed spacer 2 (ITS2) sequence analysis, were evaluated for their usefulness in inferring the population structure of Phlebotomus papatasi sand flies. ITS2 sequence analysis did not prove suitable for inferring phylogenetic and population genetic relationships across P. papatasi sand flies. Microsatellite markers showed high resolution in differentiating globally distributed P. papatasi populations, whereas cytochrome b sequence analysis provided insight into the relationships between closely related populations from the Mediterranean. Population structure, differentiation and demographic history among P. papatasi are important for understanding patterns of dispersal in this species and for planning appropriate control measures.
authors.affiliation: Department of Biological Sciences, Al-Quds University, P.O. Box 51000, Jerusalem, Palestine. ohamarsheh@science.alquds.edu
authors.forename: Omar
authors.initials: O
authors.lastname: Hamarsheh
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Presber
authors.forename: Amer
authors.initials: A
authors.lastname: Al-Jawabreh
authors.forename: Ziad
authors.initials: Z
authors.lastname: Abdeen
authors.forename: Ahmad
authors.initials: A
authors.lastname: Amro
authors.forename: Gabriele
authors.initials: G
authors.lastname: Schönian
mesh_terms: D000818:Animals; D004272:DNA, Mitochondrial / Q000235:genetics*; D004275:DNA, Ribosomal / Q000235:genetics*; D005260:Female; D005819:Genetic Markers; D014644:Genetic Variation; D016773:Leishmaniasis, Cutaneous / Q000469:parasitology; D018895:Microsatellite Repeats / Q000235:genetics; D010691:Phlebotomus / Q000145:classification / Q000235:genetics*; D017422:Sequence Analysis, DNA
chemical_list: D004272:DNA, Mitochondrial; D004275:DNA, Ribosomal; D005819:Genetic Markers