Turn-on fluorescent probe with visible light excitation for labeling of hexahistidine tagged protein.
We report here the development of a novel fluorescein-based probe which shows selective fluorescence enhancement on binding to a hexahistidine-tagged protein. No fluorescence change was observed with untagged protein. This probe is excitable with visible light and is considered to be suitable for use in biological applications.
Kamoto
Mie
M
Graduate School of Pharmaceutical Sciences, Nagoya City University, Nagoya, Aichi, Japan.
Umezawa
Naoki
N
Kato
Nobuki
N
Higuchi
Tsunehiko
T
D019793:Fluorescein / Q000138:chemical synthesis / Q000378:metabolism; D005456:Fluorescent Dyes / Q000138:chemical synthesis* / Q000378:metabolism*; D006639:Histidine / Q000737:chemistry / Q000378:metabolism*; D008027:Light; D009842:Oligopeptides / Q000737:chemistry / Q000378:metabolism*; D011485:Protein Binding; D013194:Staining and Labeling / Q000379:methods*
D005456:Fluorescent Dyes; C471213:His-His-His-His-His-His; D009842:Oligopeptides; D006639:Histidine; D019793:Fluorescein