Molecular profiles of Venezuelan isolates of Trypanosoma sp. by random amplified polymorphic DNA method.
Nine Trypanosoma sp. Venezuelan isolates, initially presumed to be T. evansi, were collected from three different hosts, capybara (Apure state), horse (Apure state) and donkey (Guarico state) and compared by the random amplification polymorphic DNA technique (RAPD). Thirty-one to 46 reproducible fragments were obtained with 12 of the 40 primers that were used. Most of the primers detected molecular profiles with few polymorphisms between the seven horse, capybara and donkey isolates. Quantitative analyses of the RAPD profiles of these isolates revealed a high degree of genetic conservation with similarity coefficients between 85.7% and 98.5%. Ten of the primers generated polymorphic RAPD profiles with two of the three Trypanosoma sp. horse isolates, namely TeAp-N/D1 and TeGu-N/D1. The similarity coefficient between these two isolates and the rest, ranged from 57.9% to 68.4% and the corresponding dendrogram clustered TeAp-N/D1 and Te Gu-N/D1 in a genetically distinct group.
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Instituto Venezolano de Investigaciones Científicas (IVIC), Centro de Biofísica y Bioquímica, Laboratorio de Fisiología de Parásitos, Km 11 Carretera Panamericana, Estado Miranda, Caracas 1020A, Venezuela.
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D000818:Animals; D001483:Base Sequence; D016054:DNA, Protozoan / Q000145:classification / Q000235:genetics*; D014644:Genetic Variation; D010802:Phylogeny; D019105:Random Amplified Polymorphic DNA Technique; D014345:Trypanosoma / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D014687:Venezuela
D016054:DNA, Protozoan