title: Regulation of mprF in daptomycin-nonsusceptible Staphylococcus aureus strains.
abstract: We used a well-characterized isogenic set of clinical bloodstream Staphylococcus aureus strains to study (i) regulation of mprF-mediated phosphatidylglycerol lysinylation in the contexts of in vitro daptomycin (DAP) nonsuceptibility and (ii) the role of mprF mutation in endovascular virulence. We observed a correlation between increased expression of a mutant mprF gene and reduced in vitro DAP susceptibility. There were no detectable fitness differences between strains in experimental infective endocarditis.
authors.affiliation: LA Biomedical Research Institute at Harbor-UCLA, 1124 West Carson Street, RB-2, Rm 230, Torrance, CA 90502, USA. sjyang@labiomed.org
authors.forename: Soo-Jin
authors.initials: SJ
authors.lastname: Yang
authors.forename: Yan Q
authors.initials: YQ
authors.lastname: Xiong
authors.forename: Paul M
authors.initials: PM
authors.lastname: Dunman
authors.forename: Jacques
authors.initials: J
authors.lastname: Schrenzel
authors.forename: Patrice
authors.initials: P
authors.lastname: François
authors.forename: Andreas
authors.initials: A
authors.lastname: Peschel
authors.forename: Arnold S
authors.initials: AS
authors.lastname: Bayer
mesh_terms: D019881:Aminoacyltransferases / Q000235:genetics* / Q000502:physiology; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000235:genetics* / Q000502:physiology; D017576:Daptomycin / Q000494:pharmacology*; D024881:Drug Resistance, Bacterial; D006801:Humans; D008826:Microbial Sensitivity Tests; D009154:Mutation; D013211:Staphylococcus aureus / Q000187:drug effects* / Q000235:genetics
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D019881:Aminoacyltransferases; C429146:mprF protein, Staphylococcus aureus; D017576:Daptomycin