Phosphate accumulation of Acetobacter xylinum.
The cells of Acetobacter xylinum decreased phosphate concentration in the medium from 5 to 2.5 or 0.3 mM during incubation in the presence of Mg(2+) and glucose, or Mg(2+) and casamino acids, respectively. The prevalence of orthophosphate or polyphosphate in the biomass of A. xylinum depends on the medium composition. Under phosphate uptake in the presence of glucose, the content of orthophosphate in the biomass changed little, while that of polyphosphate increased fourfold. At incubation with casamino acids, the content of orthophosphate increased 15 times, while that of polyphosphate increased only 2.5 times. Some part of orthophosphate in this case seems to be bound with the cell surface. The polyphosphate chain length in the cells of A. xylinim increases under phosphate uptake. This increase is more noticeable in the presence of glucose. Casamino acids can be replaced by alpha-ketoglutaric acid in combination with (NH(4))(2)SO(4), or arginine, or glutamine, the catabolism of which results in formation of NH(4) (+) and alpha-ketoglutarate.
Ryazanova
Lubov P
LP
Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Pushchino, Moscow, Russia.
Suzina
Natalia E
NE
Kulakovskaya
Tatiana V
TV
Kulaev
Igor S
IS
D000596:Amino Acids / Q000378:metabolism; D000645:Ammonium Sulfate / Q000378:metabolism; D001120:Arginine / Q000378:metabolism; D041822:Gluconacetobacter xylinus / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism; D005973:Glutamine / Q000378:metabolism; D007656:Ketoglutaric Acids / Q000378:metabolism; D008274:Magnesium / Q000378:metabolism; D010710:Phosphates / Q000378:metabolism*; D011122:Polyphosphates / Q000378:metabolism*; D000644:Quaternary Ammonium Compounds / Q000378:metabolism
D000596:Amino Acids; D007656:Ketoglutaric Acids; D010710:Phosphates; D011122:Polyphosphates; D000644:Quaternary Ammonium Compounds; C017721:casamino acids; D005973:Glutamine; D001120:Arginine; D008274:Magnesium; D005947:Glucose; D000645:Ammonium Sulfate