title: Characterization of Carbohydrates on the Surface of Second-stage Juveniles of Meloidogyne spp.
abstract: Fluorescent conjugates of the lectins soybean agglutinin (SBA), Concanavalin A (Con A), wheat germ agglutinin (WGA), Lotus tetragonolobus agglutinin (LOT), and Limulus polyphemus agglutinin (LPA) bound primarily to amphidial openings and amphidial secretions of viable, preinfective second-stage juveniles (J2) of Meloidogyne incognita races 1 and 3 (Mil, Mi3) and M. javanica (Mj). No substantial difference in fluorescent lectin binding was observed among the populations examined. Binding of only LOT and LPA were inhibited in the presence of 0.1 M competitive sugar. Structural differences in amphidial carbohydrate complexes among populations of Mi 1, Mi3, and Mj were revealed by glycohydrolase treatment of preinfective J2 and subsequent labeling with fluorescent lectins. A quantitative microfiltration enzyme-linked lectin assay revealed previously undetected differences in lectin binding to nonglycohydrolase-treated J2. Freinfective J2 of Mj bound the greatest amount of SBA, LOT, and WGA, whereas J2 of Mil bound the most LPA.
authors.forename: E L
authors.initials: EL
authors.lastname: Davis
authors.forename: D T
authors.initials: DT
authors.lastname: Kaplan
authors.forename: T A
authors.initials: TA
authors.lastname: Permar
authors.forename: D W
authors.initials: DW
authors.lastname: Dickson
authors.forename: D J
authors.initials: DJ
authors.lastname: Mitchell