Aquaporin 1 expression in retinal neovascularization in a mouse model of retinopathy of prematurity.
Recently, it was found that Aquaporin 1 (AQP1) is expressed strongly in proliferating microvessels, but the role of AQP1 in retinal neovascularization remains unknown. Here, we report the distribution of AQP1 expression during neovascularization of the retina in a mouse model of retinopathy of prematurity. AQP1 was expressed in all of the samples examined in P15 mouse and P17 mouse, including experimental and control groups. Immunostaining results showed that AQP1 is located in microvessel endothelia in retinas with proliferative retinopathy and prominently in the outer retina. Expression of AQP1 was significantly increased in experimental animals at P17, compared with control mice. No significant difference was seen in the levels of AQP1 on P12 or P15, compared with control mice. These results suggest that AQP1 may play an important role in retinal neovascularization.
Xun
Wang
W
Department of Ophthalmology, Shanghai Jiaotong University Affiliated First People's Hospital, Shanghai, P. R. China.
Liu
Yanbo
Y
Qing
Gu
G
Xun
Xu
X
Dongqing
Zhu
Z
Haixiang
Wu
W
D000818:Animals; D000831:Animals, Newborn; D051398:Aquaporin 1 / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism; D015153:Blotting, Western; D004195:Disease Models, Animal; D020869:Gene Expression Profiling; D006801:Humans; D007231:Infant, Newborn; D051379:Mice; D008810:Mice, Inbred C57BL; D012333:RNA, Messenger / Q000235:genetics; D015861:Retinal Neovascularization / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D012178:Retinopathy of Prematurity / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction
C496021:Aqp1 protein, mouse; D012333:RNA, Messenger; D051398:Aquaporin 1