title: p53-cofactor JMY is a multifunctional actin nucleation factor.
abstract: Many cellular structures are assembled from networks of actin filaments, and the architecture of these networks depends on the mechanism by which the filaments are formed. Several classes of proteins are known to assemble new filaments, including the Arp2/3 complex, which creates branched filament networks, and Spire, which creates unbranched filaments. We find that JMY, a vertebrate protein first identified as a transcriptional co-activator of p53, combines these two nucleating activities by both activating Arp2/3 and assembling filaments directly using a Spire-like mechanism. Increased levels of JMY expression enhance motility, whereas loss of JMY slows cell migration. When slowly migrating HL-60 cells are differentiated into highly motile neutrophil-like cells, JMY moves from the nucleus to the cytoplasm and is concentrated at the leading edge. Thus, JMY represents a new class of multifunctional actin assembly factor whose activity is regulated, at least in part, by sequestration in the nucleus.
authors.forename: J Bradley
authors.initials: JB
authors.lastname: Zuchero
authors.forename: Amanda S
authors.initials: AS
authors.lastname: Coutts
authors.forename: Margot E
authors.initials: ME
authors.lastname: Quinlan
authors.forename: Nicholas B La
authors.initials: NB
authors.lastname: Thangue
authors.forename: R Dyche
authors.initials: RD
authors.lastname: Mullins
mesh_terms: D051376:Actin-Related Protein 2-3 Complex / Q000378:metabolism; D000199:Actins / Q000378:metabolism*; D000595:Amino Acid Sequence; D000818:Animals; D002465:Cell Movement; D018922:HL-60 Cells; D006801:Humans; D008840:Microfilament Proteins / Q000737:chemistry / Q000378:metabolism; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000737:chemistry / Q000378:metabolism*; D021381:Protein Transport; D011554:Pseudopodia / Q000378:metabolism; D015534:Trans-Activators / Q000737:chemistry / Q000378:metabolism*; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism*
chemical_list: D051376:Actin-Related Protein 2-3 Complex; D000199:Actins; C121977:JMY protein, human; D008840:Microfilament Proteins; D009687:Nuclear Proteins; C000592292:SPIRE1 protein, human; D015534:Trans-Activators; D016159:Tumor Suppressor Protein p53