Differential proteomic analysis of subfractioned human hepatocellular carcinoma tissues.
To discover new potential biomarkers of HCC, we used 2-DE gel separation and MALDI-TOF-MS analysis of partially enriched nuclear fractions from liver biopsies of 20 different patients. We obtained a proteomic map of subfractioned liver samples including about 200 common protein spots, among which identified components corresponded to expression products of 52 different genes. A differential analysis of proteins from tumoral and control tissues revealed a significant change in the expression level of 16 proteins associated to cytoskeletal, stress response and metabolic functions. These data may provide novel candidate biomarkers for HCC and useful insights for understanding the mechanisms of HCC pathogenesis and progression.
Codarin
Erika
E
Department of Biomedical Sciences and Technologies, University of Udine, 33100 Udine, Italy.
Renzone
Giovanni
G
Poz
Alessandra
A
Avellini
Claudio
C
Baccarani
Umberto
U
Lupo
Francesco
F
di Maso
Vittorio
V
Crocè
Saveria Lory
SL
Tiribelli
Claudio
C
Arena
Simona
S
Quadrifoglio
Franco
F
Scaloni
Andrea
A
Tell
Gianluca
G