Formulation and immunological evaluation of novel vaccine delivery systems based on bioresorbable poly(ethylene glycol)-block-poly(lactide-co-epsilon-caprolactone).
Novel emulsion-type vaccine delivery systems based on the amphiphilic bioresorbable polymer poly(ethylene glycol)-block-poly(lactide-co-epsilon-caprolactone) (PEG-b-PLACL) and selected oils were developed here. Physicochemical characterizations such as stability, a droplet test, microscopic aspects, and in vitro release showed that PEG-b-PLACL-emulsified formulations have several advantages over traditional vaccine adjuvants in that they are stable, reproducible, and homogeneous fine particles with an appropriate size to facilitate the induction of potent immune responses. Different dispersion-type emulsions have provided different release profiles using ovalbumin in model studies. Immunogenicity studies in mice have shown that antigen-specific antibody titers and T-cell proliferative responses, as well as the secretion of IFN-gamma, were significantly enhanced for ovalbumin after formulation with PEG-b-PLACL-based emulsions. These features are of great interest for applications in delivery systems of prophylactic and therapeutic vaccine candidates.
Huang
Ming-Hsi
MH
Vaccine Research and Development Center, National Health Research Institutes, Zhunan, Miaoli 35053, Taiwan.
Chou
Ai-Hsiang
AH
Lien
Shu-Pei
SP
Chen
Hsin-Wei
HW
Huang
Chiung-Yi
CY
Chen
Wei-Wen
WW
Chong
Pele
P
Liu
Shih-Jen
SJ
Leng
Chih-Hsiang
CH
D000818:Animals; D016503:Drug Delivery Systems; D004655:Emulsions; D005260:Female; D006801:Humans; D007107:Immune System; D007074:Immunoglobulin G / Q000737:chemistry; D051379:Mice; D008807:Mice, Inbred BALB C; D009821:Oils / Q000737:chemistry; D010316:Particle Size; D011091:Polyesters / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry; D014612:Vaccines / Q000008:administration & dosage*
D004655:Emulsions; D007074:Immunoglobulin G; D009821:Oils; D011091:Polyesters; D011108:Polymers; D014612:Vaccines; D011092:Polyethylene Glycols; C062968:lactide-caprolactone copolymer