title: Study of apoptosis induced by cytostatics and vegetal extracts on human endothelial cell line.
abstract: Angiogenesis, the biological process by which new capillaries are formed from pre-existing vessels, is a tightly controlled and complex process involving several factors with both stimulating and inhibiting steps. In solid tumor growth, a specific clinical turning point is the transition to the vascular phase. Once it develops an intrinsic vascular network, a tumor grows indefinitely. Tumor angiogenesis depends mainly on the release by neoplasic cells of growth factors specific for endothelial cells (ECs), able to stimulate growth of the host blood vessels. The aim of this study was to analyze the apoptotic effect of some cytostatics, Vinblastine, Rapamycin and Doxorubicin, and vegetal extracts (called VOB) isolated and purified from Vitis sp., on human EA.hy926 endothelial cell line. In a proliferation assay using Crystal Violet, we demonstrated that Vinblastine and Rapamycin cytostatics have synergistic effect on endothelial cell line EA.hy926 growth inhibition. The inhibitory effects of Vinblastine and Doxorubicin were enhanced by VOB vegetal extracts. A combined treatment of cytostatics and VOB vegetal extracts resulted in a stronger antiproliferative effect of EA.hy926 endothelial cells. Results obtained regarding the apoptosis induced on EA.hy926 endothelial cells showed that each compound alone was able to induce a significant percent of apoptotic cells in a dose-dependent manner.
authors.affiliation: NIRDMI Cantacuzino, Immunomodulation Laboratory, Bucharest, Romania. sni@cantacuzino.ro
authors.forename: Simona Natalia
authors.initials: SN
authors.lastname: Bârzu
authors.forename: Maria Mihaela
authors.initials: MM
authors.lastname: Bădulescu
authors.forename: Andreea Roxana
authors.initials: AR
authors.lastname: Lupu
authors.forename: Lidia
authors.initials: L
authors.lastname: Cremer
authors.forename: G
authors.initials: G
authors.lastname: Szegli
authors.forename: F
authors.initials: F
authors.lastname: Kerek
authors.forename: Ana
authors.initials: A
authors.lastname: Călugăru
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D054697:Cytostatic Agents / Q000494:pharmacology*; D004357:Drug Synergism; D042783:Endothelial Cells / Q000187:drug effects*; D005840:Gentian Violet / Q000378:metabolism; D006801:Humans; D010936:Plant Extracts / Q000494:pharmacology*; D027843:Vitis / Q000737:chemistry*
chemical_list: D000970:Antineoplastic Agents; D054697:Cytostatic Agents; D010936:Plant Extracts; D005840:Gentian Violet