title: Upregulation of proinflammatory cytokines and NO production in BV-activated avian macrophage-like cell line (HD11) requires MAPK and NF-kappaB pathways.
abstract: The budded virus (BV) of Antheraea pernyi is a nuclear polyhedrosis virus (ApNPV), that belongs to the baculovirus family. It is capable of stimulating innate immune response in avian macrophage-like cells (HD11). In this study, we used the expression of proinflammatory cytokines (IFN-gamma and IL-12p40) and production of nitric oxide (NO) as indicators to evaluate the involvement of the signaling cascades in the activation of HD11 cells by BV. Western blot analysis revealed that ERK1/2, p38 MAPK and JNK were phosphorylated followed by activation of p65-NF-kappaB after HD11 cells were challenged with BV. Inhibition of p38 MAPK and NF-kappaB by their respective inhibitors abolished BV-induced expression of IL-12p40, IFN-gamma and production of NO, whereas inhibition of JNK abolished only the induction of cytokines. Additionally, inhibition of ERK1/2 resulted in unexpected increases in both cytokine expression and NO production. Taken together, these findings indicated that in BV-induced HD11 cells, the induction of cytokines was mediated by the p38 MAPK, JNK and NF-kappaB pathways, and NO production was mediated through the p38 MAPK/NF-kappaB pathways.
authors.affiliation: School of Environmental & Biological Science & Technology, Dalian University of Technology, RP116023 Dalian, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Han
authors.forename: Mingshan
authors.initials: M
authors.lastname: Niu
authors.forename: Lijia
authors.initials: L
authors.lastname: An
authors.forename: Wenli
authors.initials: W
authors.lastname: Li
mesh_terms: D000818:Animals; D000873:Anthracenes / Q000494:pharmacology; D001717:Birds / Q000276:immunology* / Q000821:virology; D002460:Cell Line; D004266:DNA Virus Infections / Q000276:immunology*; D007093:Imidazoles / Q000494:pharmacology; D007113:Immunity, Innate / Q000187:drug effects; D007371:Interferon-gamma / Q000235:genetics / Q000378:metabolism; D053762:Interleukin-12 Subunit p40 / Q000235:genetics / Q000378:metabolism; D048670:MAP Kinase Kinase 4 / Q000037:antagonists & inhibitors; D020935:MAP Kinase Signaling System / Q000187:drug effects / Q000276:immunology; D008262:Macrophage Activation / Q000187:drug effects; D008264:Macrophages / Q000187:drug effects / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D009036:Moths / Q000821:virology*; D009569:Nitric Oxide / Q000378:metabolism; D009570:Nitriles / Q000494:pharmacology; D017924:Nucleopolyhedroviruses / Q000276:immunology* / Q000472:pathogenicity; D010766:Phosphorylation; D011725:Pyridines / Q000494:pharmacology; D013450:Sulfones / Q000494:pharmacology; D014774:Virulence; D048051:p38 Mitogen-Activated Protein Kinases / Q000037:antagonists & inhibitors
chemical_list: C434003:3-(4-methylphenylsulfonyl)-2-propenenitrile; D000873:Anthracenes; D007093:Imidazoles; D053762:Interleukin-12 Subunit p40; D009570:Nitriles; D011725:Pyridines; D013450:Sulfones; C432165:pyrazolanthrone; D009569:Nitric Oxide; D007371:Interferon-gamma; D048051:p38 Mitogen-Activated Protein Kinases; D048670:MAP Kinase Kinase 4; C093642:SB 203580