title: Mg2+ ions bind at the C-terminal region of skeletal muscle alpha-tropomyosin.
abstract: Tropomyosin (Tm) is a dimeric coiled-coil protein that polymerizes through head-to-tail interactions. These polymers bind along actin filaments and play an important role in the regulation of muscle contraction. Analysis of its primary structure shows that Tm is rich in acidic residues, which are clustered along the molecule and may form sites for divalent cation binding. In a previous study, we showed that the Mg(2+)-induced increase in stability of the C-terminal half of Tm is sensitive to mutations near the C-terminus. In the present report, we study the interaction between Mg(2+) and full-length Tm and smaller fragments corresponding to the last 65 and 26 Tm residues. Although the smaller Tm peptide (Tm(259-284(W269))) is flexible and to large extent unstructured, the larger Tm(220-284(W269)) fragment forms a coiled coil in solution whose stability increases significantly in the presence of Mg(2+). NMR analysis shows that Mg(2+) induces chemical shift perturbations in both Tm(220-284(W269)) and Tm(259-284(W269)) in the vicinity of His276, in which are located several negatively charged residues.
authors.affiliation: Departamento de Bioquímica, Instituto de Química, Universidade de São Paulo, São Paulo, SP, Brazil.
authors.forename: Fernando
authors.initials: F
authors.lastname: Corrêa
authors.forename: Chuck S
authors.initials: CS
authors.lastname: Farah
authors.forename: Roberto K
authors.initials: RK
authors.lastname: Salinas
mesh_terms: D000595:Amino Acid Sequence; D000596:Amino Acids; D000818:Animals; D001665:Binding Sites; D002645:Chickens; D008274:Magnesium / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D018482:Muscle, Skeletal / Q000378:metabolism*; D010455:Peptides / Q000737:chemistry / Q000378:metabolism; D018583:Pliability; D011489:Protein Denaturation; D055550:Protein Stability; D013696:Temperature; D014335:Tropomyosin / Q000737:chemistry* / Q000378:metabolism*
chemical_list: D000596:Amino Acids; D010455:Peptides; D014335:Tropomyosin; D008274:Magnesium