Label-free DNA sensor based on surface charge modulated ionic conductance.
The surface charge effect in controlling ionic conductance through a nanoporous alumina membrane is investigated for its application in a convenient detection method of unlabeled DNA. To this goal, surface modification with mixtures of neutral silanes and morpholinos (neutral analogues of DNA) was optimized to yield a strong effect on ionic conductance change upon DNA binding, which can exceed an order of magnitude. The effect can be employed in fabrication of inexpensive DNA sensors.
Wang
Xian
X
Department of Chemistry and Biochemistry, New Mexico State University, Las Cruces, New Mexico 88003, USA.
Smirnov
Sergei
S
D000537:Aluminum Oxide / Q000737:chemistry; D015374:Biosensing Techniques; D004247:DNA / Q000032:analysis*; D004553:Electric Conductivity; D008855:Microscopy, Electron, Scanning; D049329:Nanostructures / Q000737:chemistry / Q000648:ultrastructure; D009994:Osmolar Concentration; D013499:Surface Properties
D004247:DNA; D000537:Aluminum Oxide