title: Pentoxifylline modifies three-dimensional collagen lattice model contraction and expression of collagen types I and III by human fibroblasts derived from post-burn hypertrophic scars and from normal skin.
abstract: Fibroblasts are thought to be partially responsible for the persisting contractile forces that result in burn contractures. Using a monolayer cell culture and fibroblast populated collagen lattice (FPCL) three-dimensional model we subjected hypertrophic scar and non-cicatricial fibroblasts to the antifibrogenic agent pentoxifylline (PTF - 1mg/mL) in order to reduce proliferation, collagen types I and III synthesis and model contraction. Fibroblasts were isolated from post-burn hypertrophic scars (HSHF) and non-scarred skin (NHF). Cells were grown in monolayers or incorporated into FPCL's and exposed to PTF. In monolayer, cell number proliferation was reduced (46.35% in HSHF group and 37.73% in NHF group, p<0.0001). PTF selectively inhibited collagen III synthesis in the HSHF group while inhibition was more evident to type I collagen synthesis in the NHF group. PTF also reduced contraction in both (HSHF and NHF) FPCL.
authors.affiliation: Faculty of Medicine, Cell Culture and Wound Healing Research Laboratory, Division of Plastic Surgery, University of São Paulo, Avenida Doutor Arnaldo 455 sala 1360, São Paulo, São Paulo, Brazil. cesaris@plastica.fm.usp.br
authors.forename: Cesar
authors.initials: C
authors.lastname: Isaac
authors.forename: Mônica Beatriz
authors.initials: MB
authors.lastname: Mathor
authors.forename: Giovani
authors.initials: G
authors.lastname: Bariani
authors.forename: André Oliveira
authors.initials: AO
authors.lastname: Paggiaro
authors.forename: Marisa Roma
authors.initials: MR
authors.lastname: Herson
authors.forename: Claudia
authors.initials: C
authors.lastname: Goldenstein-Schainberg
authors.forename: Solange
authors.initials: S
authors.lastname: Carrasco
authors.forename: Walcy Rosolia
authors.initials: WR
authors.lastname: Teodoro
authors.forename: Natalino Hajime
authors.initials: NH
authors.lastname: Yoshinari
authors.forename: Marcus Castro
authors.initials: MC
authors.lastname: Ferreira
mesh_terms: D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D017439:Cicatrix, Hypertrophic / Q000473:pathology*; D024042:Collagen Type I / Q000096:biosynthesis; D024061:Collagen Type III / Q000096:biosynthesis; D003286:Contracture / Q000473:pathology*; D004353:Drug Evaluation, Preclinical; D005347:Fibroblasts / Q000187:drug effects* / Q000378:metabolism; D006801:Humans; D010431:Pentoxifylline / Q000494:pharmacology*; D010726:Phosphodiesterase Inhibitors / Q000494:pharmacology*; D012867:Skin / Q000166:cytology / Q000187:drug effects / Q000378:metabolism
chemical_list: D024042:Collagen Type I; D024061:Collagen Type III; D010726:Phosphodiesterase Inhibitors; D010431:Pentoxifylline