[Protective effect of glial cell line-derived neurotrophic factor infused into the tube setted into cavitas subarachnoidealis on spinal front corner motor neurons].
OBJECTIVE
To investigate the effect of exogenous glial cell line-derived neurotrophic factor (GDNF) infused into the cavitas subarachnoidealis on cornu anterius medullae spinalis motor neurons after sciatic nerve axotomy.


METHODS
Forty-eight healthy SD rats were divided into 2 groups randomly: GDNF group and NS group. The left sciatic nerve in rats were cut off. And then 0.9% saline (6 microl) and GDNF solution (6 microl) were injected into cavitas subarachnoidealis at L4-L6 in NS group and GDNF group,respectively. The rats were sacrificed on postoperative 1, 2, 4 and 8 weeks respectively. Their specimen of L4-L6 spinal cord were taken at different time and sectioned. The HE staining, Nissl staining and cholinesterase (ChE) staining in motor neurons were used for counting of motor neurons.


RESULTS
In GDNF group the number of motor neurons in cornu anterius medullae spinalis and the ChE activity were higher than that of NS group.


CONCLUSIONS
The exogenous GDNF infused into the cavitas subarachnoidealis are supposed to protect the degenerated spinal motor neuron from death after sciatic nerve injury.
Pan
Shi-peng
SP
Department of Orthopaedics, the Center Hospital of Zhongtie 17 Ju, Taiyuan 030032, Shanxi, China. canmobu@163.com
Liu
Qiang
Q
Wu
Dou
D
D000818:Animals; D019771:Axotomy; D002802:Cholinesterases / Q000378:metabolism; D051100:Glial Cell Line-Derived Neurotrophic Factor / Q000494:pharmacology*; D008297:Male; D009046:Motor Neurons / Q000187:drug effects* / Q000378:metabolism; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D012584:Sciatic Nerve / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D013116:Spinal Cord / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism / Q000601:surgery
D051100:Glial Cell Line-Derived Neurotrophic Factor; D002802:Cholinesterases