[Isolation and characterization of human rheumatoid arthritis fibroblast-like synoviocytes].
OBJECTIVE
To isolate and characterize human rheumatoid arthritis (RA) fibroblast-like synoviocytes (FLSs).


METHODS
The synovial membrane tissues were obtained from 4 RA patients, 1 chondroma patient and 1 healthy subject and FLS were isolated by means of tissue culture. The cell morphology was observed by phase-contrast microscope and the cell surface markers were detected by flow cytometry.


RESULTS
The FLSs were successfully cultured from the synovial membrane tissues with good cell homogeneity after the third passage. The FLSs of the 3rd to 7th passages were stable and proliferated actively, followed by slow proliferation and aging since the 8th passage. Flow cytometry showed that the 4th-passage FLSs from the RA patients contained 99.04% CD90(+) cells, 2.73% CD3(+) cells, 0.29% CD3(-)CD19(+) cells, 2.81% CD3(-)CD16(+)CD56(+) cells, 5.89% CD14(+) cells, and 54.17% CD55(+) cells. The presence of interleukin-1 receptor type I (IL-1RI, 158.63-/+20.32 pg/ml) and IL-1beta (4.67-/+0.82 pg/ml) were detected in the cell culture supernatant of the 4th-passage FLSs from the RA patients by enzyme-linked immunosorbent assay ELISA.


CONCLUSIONS
FLSs from RA patients can be effectively culture by means of tissue culture, and the cultured FLSs show high expressions of CD90, IL-1RI and IL-1beta.
Huang
Xian-Zhang
XZ
Laboratory Medicine Center, Nangfang Hospital, Southern Medical University, Guangzhou, China. xianzhanghuang@yahoo.com.cn
Wang
Qian
Q
Zheng
Lei
L
Chen
Xiao
X
Xiao
Ping
P
Xiong
Shi-Long
SL
Bao
Jie
J
Ding
Hai-Ming
HM
Huang
Wu-Jiao
WJ
Zhuang
Jun-Hua
JH
D000328:Adult; D000368:Aged; D001172:Arthritis, Rheumatoid / Q000473:pathology*; D049109:Cell Proliferation; D002469:Cell Separation; D002478:Cells, Cultured; D005260:Female; D005347:Fibroblasts / Q000473:pathology*; D006801:Humans; D053583:Interleukin-1beta / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D053573:Receptors, Interleukin-1 Type I / Q000378:metabolism; D013583:Synovial Membrane / Q000166:cytology* / Q000473:pathology; D018800:Thy-1 Antigens / Q000378:metabolism
D053583:Interleukin-1beta; D053573:Receptors, Interleukin-1 Type I; D018800:Thy-1 Antigens