title: Investigation of bacteria with polyketide synthase genes and antimicrobial activity isolated from South China Sea sponges.
abstract: OBJECTIVE
  To obtain bacteria with PKS (polyketide synthase) genes and antimicrobial activity from sponges.
  
  
  RESULTS
  Eighteen bacteria with KS (ketosynthase) genes were identified by polymerase chain reaction (PCR) screening of 98 isolates from South China Sea sponges, Stelletta tenuis, Halichondria rugosa, Dysidea avara and Craniella australiensis. 16S rRNA gene-based Blast analysis indicated that 15 isolates belonged to the phylum Firmicutes, among which 14 isolates were closely related to genus Bacillus, and 1 to Staphylococcus lentus. Two isolates were identified as actinomycetes, and one as Alcaligenes sp. in the phylum Proteobacteria. The 18 KS domains belong to trans-AT type I PKS and match PKS of marine bacterial symbionts. The 18 bacteria exhibited broad-spectrum antimicrobial activities against fungi, gram-positive and gram-negative bacteria. A 21.8-kb PKS gene cluster fragment containing five modules was isolated from the Staphylococcus lentus isolate A75 by screening of a fosmid library.
  
  
  CONCLUSIONS
  The PKS gene diversity and different antimicrobial spectra indicate the potential of bacteria associated with South China Sea sponges for diverse polyketide production.
  
  
  CONCLUSIONS
  Combined with bioactivity assay the PKS gene-based approach can be applied to efficient screening of strains of pharmaceutical value and the prediction of related compounds.
authors.affiliation: Laboratory of Marine Biotechnology, School of Life Science, Ministry of Education, Shanghai Jiao Tong University, Shanghai, PR China.
authors.forename: W
authors.initials: W
authors.lastname: Zhang
authors.forename: F
authors.initials: F
authors.lastname: Zhang
authors.forename: Z
authors.initials: Z
authors.lastname: Li
authors.forename: X
authors.initials: X
authors.lastname: Miao
authors.forename: Q
authors.initials: Q
authors.lastname: Meng
authors.forename: X
authors.initials: X
authors.lastname: Zhang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000890:Anti-Infective Agents / Q000494:pharmacology*; D001419:Bacteria / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics / Q000302:isolation & purification*; D002681:China; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics; D005658:Fungi / Q000187:drug effects; D015723:Gene Library; D008969:Molecular Sequence Data; D009792:Oceans and Seas; D010802:Phylogeny; D048630:Polyketide Synthases / Q000096:biosynthesis* / Q000494:pharmacology; D016133:Polymerase Chain Reaction; D011161:Porifera / Q000382:microbiology*; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D000890:Anti-Infective Agents; D004269:DNA, Bacterial; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D048630:Polyketide Synthases