title: Dissecting the bacterial type VI secretion system by a genome wide in silico analysis: what can be learned from available microbial genomic resources?
abstract: BACKGROUND
  The availability of hundreds of bacterial genomes allowed a comparative genomic study of the Type VI Secretion System (T6SS), recently discovered as being involved in pathogenesis. By combining comparative and phylogenetic approaches using more than 500 prokaryotic genomes, we characterized the global T6SS genetic structure in terms of conservation, evolution and genomic organization.
  
  
  RESULTS
  This genome wide analysis allowed the identification of a set of 13 proteins constituting the T6SS protein core and a set of conserved accessory proteins. 176 T6SS loci (encompassing 92 different bacteria) were identified and their comparison revealed that T6SS-encoded genes have a specific conserved genetic organization. Phylogenetic reconstruction based on the core genes showed that lateral transfer of the T6SS is probably its major way of dissemination among pathogenic and non-pathogenic bacteria. Furthermore, the sequence analysis of the VgrG proteins, proposed to be exported in a T6SS-dependent way, confirmed that some C-terminal regions possess domains showing similarities with adhesins or proteins with enzymatic functions.
  
  
  CONCLUSIONS
  The core of T6SS is composed of 13 proteins, conserved in both pathogenic and non-pathogenic bacteria. Subclasses of T6SS differ in regulatory and accessory protein content suggesting that T6SS has evolved to adapt to various microenvironments and specialized functions. Based on these results, new functional hypotheses concerning the assembly and function of T6SS proteins are proposed.
authors.affiliation: CEA, iRTSV, Laboratoire Biologie, Informatique et Mathématiques, Grenoble, France. frederic.boyer@cea.fr
authors.forename: Frédéric
authors.initials: F
authors.lastname: Boyer
authors.forename: Gwennaële
authors.initials: G
authors.lastname: Fichant
authors.forename: Jérémie
authors.initials: J
authors.lastname: Berthod
authors.forename: Yves
authors.initials: Y
authors.lastname: Vandenbrouck
authors.forename: Ina
authors.initials: I
authors.lastname: Attree
mesh_terms: D001419:Bacteria / Q000145:classification / Q000235:genetics* / Q000378:metabolism; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D002874:Chromosome Mapping; D019143:Evolution, Molecular; D005798:Genes, Bacterial; D016680:Genome, Bacterial; D023281:Genomics; D005810:Multigene Family; D010802:Phylogeny; D021381:Protein Transport
chemical_list: D001426:Bacterial Proteins