Decreased proliferation of mesenchymal stem cells in corticosteroid-induced osteonecrosis of femoral head.
The ability of self-repair in patients with corticosteroid-induced osteonecrosis of the femoral head is limited, and it has been suggested the cause is likely relevant to the poor proliferation activity of mesenchymal stem cells in the femoral head region. This study measured the number and proliferation activity of human mesenchymal stem cells in patients both with and without corticosteroid-induced osteonecrosis of the femoral head. Bone marrow was collected from the proximal femur in patients with steroid-induced osteonecrosis of the femoral head (osteonecrosis group, n=18) and patients with new femoral neck fractures without osteonecrosis (control group, n=11). Mesenchymal stem cells were isolated by density gradient centrifugation, and then selected by the adhesive method. The MTT reduction assay method was used to evaluate the level of proliferation. Cells from osteonecrosis patients showed reduced proliferation ability compared with the control patients. The percentage of cells in the S+G2/M phase was decreased significantly (P<.01) in the osteonecrosis group. The decreased proliferation ability of mesenchymal stem cells may play a role in the low repair capacity of steroid-induced osteonecrosis of femoral head. The altered function of mesenchymal stem cells may be responsible for the pathogenesis and progression of osteonecrosis.
Wang
Bai-Liang
BL
Department of Orthopedic Surgery, Osteonecrosis and Joint-Preserving and Reconstruction Center, China-Japan Friendship Hospital, Beijing 100029, China.
Sun
Wei
W
Shi
Zhen-Cai
ZC
Lou
Jin-Ning
JN
Zhang
Nian-Fei
NF
Shi
Shao-Hui
SH
Guo
Wan-Shou
WS
Cheng
Li-Ming
LM
Ye
Li-Ya
LY
Zhang
Wen-Jian
WJ
Li
Zi-Rong
ZR
D000305:Adrenal Cortex Hormones / Q000009:adverse effects*; D000368:Aged; D000369:Aged, 80 and over; D049109:Cell Proliferation; D005271:Femur Head Necrosis / Q000139:chemically induced* / Q000473:pathology*; D006801:Humans; D008297:Male; D059630:Mesenchymal Stem Cells / Q000473:pathology*; D008875:Middle Aged
D000305:Adrenal Cortex Hormones