title: Determination of malathion levels and the effect of malathion on the growth of Chrysomya megacephala (Fibricius) in malathion-exposed rat carcass.
abstract: This study was conducted to examine the effect of malathion on the development of Chrysomya megacephala. A total of 12 adult Sprague-Dawley rats was divided into 4 groups. Each animal in the 4 groups was given orally 0 (control), 10, 25 and 50ml/kg body weight of malathion, respectively. Chrysomya megacephala larvae were then allowed to grow on the liver of carcass. Larvae development was estimated by means of weight and length, time of adult emergence and survival rate. Results indicated that for the first 6 to 30 hours, larvae from control group developed more rapidly than larvae feeding on tissue containing malathion. However, the 3 doses of malathion did not exhibit significant impact on larvae length and weight. The time required for adult emergence was significantly greater for malathion-treated colony which was 10 days compared to 7 days in control colony. Control larvae of C. megacephala had higher survival rate compared to larvae exposed to the three different doses of malathion. Analysis of the tissues indicated that all rats and fly samples were positive for malathion. Malathion concentration was highest in liver. It was concluded that the presence of malathion altered the development rate of C. megacephala and thus disrupted normal postmortem interval estimation.
authors.affiliation: Forensic Science Programme, Faculty of Allied Health Science, Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz, 50300 Kuala Lumpur.
authors.forename: Rashid
authors.initials: R
authors.lastname: Rumiza Abd
authors.forename: Khairul
authors.initials: K
authors.lastname: Osman
authors.forename: Ismail
authors.initials: I
authors.lastname: Mohd Iswadi
authors.forename: Zuha
authors.initials: Z
authors.lastname: Raja Muhammad
authors.forename: Hassan
authors.initials: H
authors.lastname: Rogaya Abu
mesh_terms: D000818:Animals; D004175:Diptera / Q000187:drug effects* / Q000254:growth & development; D004772:Entomology; D044707:Forensic Sciences / Q000379:methods*; D007306:Insecticides / Q000493:pharmacokinetics*; D007814:Larva / Q000187:drug effects / Q000254:growth & development; D008294:Malathion / Q000493:pharmacokinetics* / Q000494:pharmacology; D011180:Postmortem Changes; D051381:Rats; D017207:Rats, Sprague-Dawley; D014018:Tissue Distribution
chemical_list: D007306:Insecticides; D008294:Malathion