title: Endothelial nitric oxide synthase (eNOS) Glu298-->Asp polymorphism (G894T) among south Indians.
abstract: OBJECTIVE
  Nitric oxide (NO) synthesized by endothelial cells is known to be a potent vasodilator. It has been suggested that the single nucleotide polymorphism (SNP) in endothelial nitric oxide synthase (eNOS) (G894T) may affect the response of vascular endothelium to increased oxidative stress. The Glu298Asp (GT) polymorphism located in exon 7, may be associated with several diseases. The objective of the present study was to determine the presence of genotype frequencies of Glu298-->Asp (G894T) single nucleotide polymorphism in the eNOS gene among south Indian male Tamil speaking population.
  
  
  METHODS
  Polymerase chain reaction and restriction fragmant analysis was done to detect the presence of Glu298-->Asp (G894T) variant of the eNOS gene in 105 healthy male volunteers.
  
  
  RESULTS
  The frequency of the eNOS GG, GT, and TT genotypes was found to be 74.3, 25.7, and 0 per cent respectively. The 'T' allele frequency among this population was found to be 0.13. Chi square analysis showed that the study population lies in accordance with Hardy-Weinberg equilibrium.
  
  
  CONCLUSIONS
  The occurrence of the homozygous mutant (TT) was completely absent among the study population. Moreover, our result indicated that the presence of the homozygous mutant genotype for eNOS polymorphism was absent/less common among south Indian male Tamil population of Asian race.
authors.affiliation: PG & Research Department of Zoology & Biotechnology, Lady Doak College, Madurai, India.
authors.forename: N S
authors.initials: NS
authors.lastname: Nishevitha
authors.forename: T
authors.initials: T
authors.lastname: Angeline
authors.forename: Nirmala
authors.initials: N
authors.lastname: Jeyaraj
mesh_terms: D001483:Base Sequence; D019295:Computational Biology; D005006:Ethnicity / Q000235:genetics*; D005787:Gene Frequency; D005828:Genetics, Population; D005838:Genotype; D006801:Humans; D007194:India; D008297:Male; D008969:Molecular Sequence Data; D052250:Nitric Oxide Synthase Type III / Q000235:genetics*; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D017422:Sequence Analysis, DNA
chemical_list: D052250:Nitric Oxide Synthase Type III