The ribozyme core of group II introns: a structure in want of partners.
Group II introns contain a large ribozyme, which catalyzes self-splicing, and the coding sequence of a reverse transcriptase, the function of which is to cooperate with the ribozyme to achieve genomic mobility. Despite its lack of substrates for both steps of the splicing process, the crystal structure of a group II ribozyme reveals the location of two metal ions most likely to be involved in catalysis; the RNA structure that binds to these ions results from the bending of a local motif by the folding of the rest of the ribozyme. The stage is now set to determine where the intron-encoded protein binds to its partner and whether the spliceosome uses a counterpart of the group II catalytic center to excise nuclear pre-messenger introns.
Michel
François
F
Centre de Génétique Moléculaire du CNRS, 1 Avenue de la Terrasse, 91190 Gif-sur-Yvette, France. francois.michel@cgm.cnrs-gif.fr
Costa
Maria
M
Westhof
Eric
E
D017398:Alternative Splicing / Q000235:genetics; D007438:Introns / Q000235:genetics*; D016337:RNA, Catalytic / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D017381:Spliceosomes / Q000378:metabolism
D016337:RNA, Catalytic