title: PELDOR spectroscopy distance fingerprinting of the octameric outer-membrane protein Wza from Escherichia coli.
abstract: Distance fingerprinting: Pulsed electron-electron double resonance spectroscopy (PELDOR) is applied to the octameric membrane protein complex Wza of E. coli. The data yielded a detailed distance fingerprint of its periplasmic region that compares favorably to the crystal structure. These results provide the foundation to study conformation changes from interaction with partner proteins.
authors.affiliation: Centre for Biomolecular Sciences, The University of St. Andrews, Fife KY16 9RH, UK.
authors.forename: Gregor
authors.initials: G
authors.lastname: Hagelueken
authors.forename: W John
authors.initials: WJ
authors.lastname: Ingledew
authors.forename: Hexian
authors.initials: H
authors.lastname: Huang
authors.forename: Biljana
authors.initials: B
authors.lastname: Petrovic-Stojanovska
authors.forename: Chris
authors.initials: C
authors.lastname: Whitfield
authors.forename: Hassane
authors.initials: H
authors.lastname: ElMkami
authors.forename: Olav
authors.initials: O
authors.lastname: Schiemann
authors.forename: James H
authors.initials: JH
authors.lastname: Naismith
mesh_terms: D019943:Amino Acid Substitution; D001425:Bacterial Outer Membrane Proteins / Q000737:chemistry*; D003198:Computer Simulation; D018360:Crystallography, X-Ray; D004578:Electron Spin Resonance Spectroscopy; D004926:Escherichia coli / Q000737:chemistry*; D029968:Escherichia coli Proteins / Q000737:chemistry*; D050505:Mutant Proteins / Q000737:chemistry; D019897:Periplasm / Q000378:metabolism; D017434:Protein Structure, Tertiary
chemical_list: D001425:Bacterial Outer Membrane Proteins; D029968:Escherichia coli Proteins; D050505:Mutant Proteins; C404158:Wza protein, E coli