title: Prognostic significance of immunohistochemical classification of diffuse large B-cell lymphoma.
abstract: OBJECTIVE
  To evaluate the clinical significance of immunoperoxidase staining for CD10, bcl-6, mum-1 and bcl-2 to subdivide DLBCL into prognostic subgroups, we analysed 50 DLBCL cases using immunohistochemical methods.
  
  
  RESULTS
  Fifty DLBCL patients were evaluated retrospectively. The expression of CD10 was associated with better OS (p=0.04) whereas expression of mum-1 was associated with worse OS (p=0.009). There were no significance of OS in case of expression of bcl-6 (p=0.05) and bcl-2 (p=0.3). They were subclassified using CD10, mum-1, bcl-6 as germinal center B-cell like (GCB) lymphoma (30%) and non-GCB lymphoma (70%). The OS and EFS (event free survival) were longer in GCB group (p=0.002) and 5-year OS for GCB group was 92% compared with only 44% for the non-GCB group (p=0.02). The OS of the GCB group also was longer compared to that of the non-GCB group in low IPI subgroup (p=0.01).
  
  
  CONCLUSIONS
  The existence of survival differences between GCB a non-GCB group also in the patients with low IPI score, showed the importance of prognostic classification in the risk-adaptive treatment approaches. The classification as GCB and non-GCB based immunostains may enable to define more accurate prognostic groups in DLBCL.
authors.affiliation: Division of Hematology, Dokuz Eylul University Faculty of Medicine, Balcova, Izmir, Turkey.
authors.forename: Inci
authors.initials: I
authors.lastname: Alacacioglu
authors.forename: Mehmet Ali
authors.initials: MA
authors.lastname: Ozcan
authors.forename: Sermin
authors.initials: S
authors.lastname: Ozkal
authors.forename: Ozden
authors.initials: O
authors.lastname: Piskin
authors.forename: Nurhilal
authors.initials: N
authors.lastname: Turgut
authors.forename: Fatih
authors.initials: F
authors.lastname: Demirkan
authors.forename: Guner Hayri
authors.initials: GH
authors.lastname: Ozsan
authors.forename: Aydanur
authors.initials: A
authors.lastname: Kargi
authors.forename: Bulent
authors.initials: B
authors.lastname: Undar
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D018572:Disease-Free Survival; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D007150:Immunohistochemistry / Q000379:methods; D016403:Lymphoma, Large B-Cell, Diffuse / Q000145:classification* / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D008297:Male; D008875:Middle Aged; D011379:Prognosis; D040081:Protein Array Analysis; D012189:Retrospective Studies; D015996:Survival Rate; D055815:Young Adult