title: Cloning and characterization of 60S ribosomal protein L22 (RPL22) from Culex pipiens pallens.
abstract: The 60S ribosomal protein L22 (GenBank accession no. EF990190) was cloned from Culex pipiens pallens. An open reading frame (ORF) of 447 bps was found to encode a putative 148 amino acids protein which shares 90% and 80% identity with RPL22 genes from Aedes aegypti and Anopheles gambiae respectively. Real-time quantitative PCR analysis demonstrated that the transcription level of RPL22 in deltamethrin-resistant strain was 2.57 folds higher than in deltamethrin-susceptible strain of Cx. pipiens pallens. Overexpression of RPL22 in C6/36 cells showed that the deltamethrin-resistance was decreased in C6/36-RPL22 cell compared to the control. The mRNA level of cytochrome P450 6A1 (CYP6A1, GenBank accession no. FJ423553) showed that CYP6A1 was down-regulated in the C6/36 transfected with RPL22 (C6/36-RPL22) cells, suggesting that CYP6A1 was repressed by RPL22. Our study provides the first evidence that RPL22 may play some role in the regulation of deltamethrin-resistance in Cx. pipiens pallens.
authors.affiliation: Department of Pathogen Biology, Nanjing Medical University, Nanjing, Jiangsu 210029, PR China.
authors.forename: Ji
authors.initials: J
authors.lastname: He
authors.forename: Haibo
authors.initials: H
authors.lastname: Sun
authors.forename: Donghui
authors.initials: D
authors.lastname: Zhang
authors.forename: Yan
authors.initials: Y
authors.lastname: Sun
authors.forename: Lei
authors.initials: L
authors.lastname: Ma
authors.forename: Lin
authors.initials: L
authors.lastname: Chen
authors.forename: Zhengxia
authors.initials: Z
authors.lastname: Liu
authors.forename: Chunrong
authors.initials: C
authors.lastname: Xiong
authors.forename: Guiyun
authors.initials: G
authors.lastname: Yan
authors.forename: Changliang
authors.initials: C
authors.lastname: Zhu
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002478:Cells, Cultured; D003001:Cloning, Molecular; D003465:Culex / Q000378:metabolism*; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics / Q000378:metabolism*; D005786:Gene Expression Regulation; D019476:Insect Proteins / Q000235:genetics / Q000378:metabolism*; D007306:Insecticides / Q000494:pharmacology; D008969:Molecular Sequence Data; D009570:Nitriles / Q000494:pharmacology; D016366:Open Reading Frames / Q000235:genetics; D010802:Phylogeny; D011722:Pyrethrins / Q000494:pharmacology; D012269:Ribosomal Proteins / Q000235:genetics / Q000378:metabolism*; D017386:Sequence Homology, Amino Acid
chemical_list: D019476:Insect Proteins; D007306:Insecticides; D009570:Nitriles; D011722:Pyrethrins; D012269:Ribosomal Proteins; C017180:decamethrin; D003577:Cytochrome P-450 Enzyme System