title: Engraftment of human blood malignancies to the turkey embryo: a robust new in vivo model.
abstract: Xenografting of human blood malignancies to immunodeficient SCID mice is a powerful research tool. We evaluate here whether the immunodeficient turkey embryo can also serve as a xenograft host for human blood malignancies. Human leukemia, lymphoma and myeloma lines engrafted robustly into medullary and extramedullary tissues of turkey embryos as detected by PCR, FACS and histology in 8-10 days. Four of eleven patient AML samples also engrafted the bone marrow. Grafts of two lines responded to chemotherapy with doxorubicin. The turkey embryo therefore has the potential to be a complementary xenograft model for the study of human blood malignancies.
authors.affiliation: Mina and Everard Goodman Faculty of Life Sciences, Bar-Ilan University, Ramat-Gan, Israel.
authors.forename: Igor
authors.initials: I
authors.lastname: Grinberg
authors.forename: Arbel
authors.initials: A
authors.lastname: Reis
authors.forename: Avivit
authors.initials: A
authors.lastname: Ohana
authors.forename: Moran
authors.initials: M
authors.lastname: Taizi
authors.forename: Michal
authors.initials: M
authors.lastname: Cipok
authors.forename: Sigal
authors.initials: S
authors.lastname: Tavor
authors.forename: Deborah
authors.initials: D
authors.lastname: Rund
authors.forename: Varda R
authors.initials: VR
authors.lastname: Deutsch
authors.forename: Ronald S
authors.initials: RS
authors.lastname: Goldstein
mesh_terms: D000818:Animals; D001803:Blood Transfusion / Q000379:methods; D045744:Cell Line, Tumor / Q000637:transplantation*; D002642:Chick Embryo; D002645:Chickens; D017931:DNA Primers; D006801:Humans; D007231:Infant, Newborn; D007938:Leukemia; D008223:Lymphoma; D009101:Multiple Myeloma; D009368:Neoplasm Transplantation / Q000276:immunology / Q000379:methods*; D016133:Polymerase Chain Reaction; D013045:Species Specificity; D014183:Transplantation, Heterologous / Q000276:immunology / Q000379:methods; D014422:Turkeys / Q000196:embryology*
chemical_list: D017931:DNA Primers