Neuroactive flavonoid glycosides from Tilia petiolaris DC. extracts.
Pharmacological assay guided purification of an ethanol extract of Tilia petiolaris DC. inflorescences resulted in the isolation and identification of isoquercitrin (ISO), quercetin 3-O-glucoside-7-O-rhamnoside (QUE) and kaempferol 3-O-glucoside-7-O-rhamnoside (KAE). The behavioral actions of these glycosylated flavonoids were examined in the hole board, locomotor activity and thiopental-induced loss of righting reflex tests in mice. QUE (10 and 30 mg/kg) and KAE (30 mg/kg), intraperitoneally (i.p.) administered to mice, reduced all the parameters measured in the hole board test, but ISO (30 mg/kg) only reduced the number of rearings. Meanwhile QUE at 30 mg/kg i.p. also decreased the ambulatory locomotor activity and increased the sodium thiopental-induced time of loss of the righting reflex suggesting a clear depressant action. The above results demonstrate the occurrence of neuroactive flavonoid glycosides in Tilia.
Loscalzo
Leonardo M
LM
Instituto de Química y Fisicoquímica Biológicas, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Buenos Aires, Argentina.
Wasowski
Cristina
C
Marder
Mariel
M
D000818:Animals; D001522:Behavior, Animal / Q000187:drug effects*; D005419:Flavonoids / Q000302:isolation & purification / Q000494:pharmacology; D005960:Glucosides / Q000302:isolation & purification / Q000494:pharmacology*; D006031:Glycosylation; D006993:Hypnotics and Sedatives / Q000302:isolation & purification / Q000494:pharmacology*; D056626:Inflorescence; D044949:Kaempferols / Q000302:isolation & purification / Q000494:pharmacology*; D008297:Male; D051379:Mice; D009043:Motor Activity / Q000187:drug effects*; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D011597:Psychomotor Performance / Q000187:drug effects*; D011794:Quercetin / Q000031:analogs & derivatives* / Q000302:isolation & purification / Q000494:pharmacology; D032384:Tilia / Q000737:chemistry*
D005419:Flavonoids; D005960:Glucosides; D006993:Hypnotics and Sedatives; D044949:Kaempferols; D010936:Plant Extracts; C546610:kaempferol 3-O-glucoside-7-O-rhamnoside; C546609:quercetin 3-O-glucoside-7-O-rhamnoside; C016527:isoquercitrin; D011794:Quercetin