Protein-free ligand screening: simplification of chiral chromatographic development via novel adaptation of NMR screening methodologies.
We demonstrate here a promising NMR method that provides evidence for chiral compound selector interaction as a first-pass screening method. A novel adaptation of commonly used protein-based screening technologies, this approach relies upon ligand-to-stationary phase interaction wherein the stationary phase is tethered to sepharose beads. At only minutes per experiment, this methodology significantly reduces the time required for chiral separation methodology development and complements currently available chromatographic purity technologies.
Thompson
Melissa R
MR
Pfizer Global Research and Development, St. Louis Laboratories, Saint Louis, MO, USA. Melissa.Thompson@Pfizer.com
McKenzie
David R
DR
Likos
John J
JJ
Gard
Janice K
JK
D002845:Chromatography; D007536:Isomerism; D008024:Ligands; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods*; D011506:Proteins / Q000737:chemistry*
D008024:Ligands; D011506:Proteins