Anti-hemorrhagic activity of four Brazilian vegetable species against Bothrops jararaca venom.
Around 20,000 snakebites are reported annually in Brazil and 90% of them are inflicted by species of the genus Bothrops. Intravenous administration of antibothropic antivenom neutralizes the systemic actions, but it is of little effect on the reversal of local symptoms and often induces adverse reactions, a context that drives the search for complementary treatments for snakebite accidents. Vegetable extracts with a range of antiophidian activities constitute an excellent alternative. In this study, we investigated the anti-hemorrhagic effects of Mouriri pusa Gardn. (Melastomataceae), Byrsonima crassa Niedenzu (Malpighiaceae), Davilla elliptica St. Hill. (Dilleniaceae) and Strychnos pseudoquina St. Hil. (Loganiaceae) against Bothrops jararaca venom. The methanolic extracts from M. pusa (leaves), B. crassa (leaves) and D. elliptica (leaves) showed total neutralization capacity against local hemorrhages. The amenthoflavone and quercetin fractions from B. crassa and the flavonoids fractions (quercetin and myricetin) from M. pusa and D. elliptica also showed total neutralization capacity. We conclude that flavonoids derived from myricetin, quercetin and amenthoflavone play an important role in the anti-hemorrhagic potential of these Brazilian vegetables species against B. jararaca venom.
Nishijima
Catarine Massucato Nishijima
CM
Department of Physiology, São Paulo State University, UNESP/Botucatu, SP, Brazil.
Rodrigues
Clenilson Martins
CM
Silva
Marcelo Aparecido
MA
Lopes-Ferreira
Mônica
M
Vilegas
Wagner
W
Hiruma-Lima
Clélia Akiko
CA
D000818:Animals; D000992:Antitoxins / Q000494:pharmacology; D017837:Bothrops; D005419:Flavonoids / Q000494:pharmacology* / Q000627:therapeutic use; D006470:Hemorrhage / Q000139:chemically induced / Q000188:drug therapy*; D006801:Humans; D010936:Plant Extracts / Q000494:pharmacology / Q000627:therapeutic use*; D011794:Quercetin; D012909:Snake Bites / Q000188:drug therapy*; D014675:Vegetables / Q000737:chemistry; D014757:Viper Venoms / Q000009:adverse effects*
D000992:Antitoxins; D005419:Flavonoids; D010936:Plant Extracts; D014757:Viper Venoms; C040015:myricetin; D011794:Quercetin