Identification and isolation of pharmacologically active triterpenes in Betuale cortex, Betula pendula Roth., Betulaceae.
Betulae cortex, Betula pendula Roth., Betulaceae, comprise triterpene substances which are confirmed to posses very important pharmacological activities such as anti-inflammatory, anticancer and antiviral. In this study, extraction of triterpene substances from both, inner and external birch bark was carried out and after that qualitative analysis on betulin, betulinic acid, oleanolic acid and lupeol was performed by method of thin layer chromatography. By this separation method, applying system for development benzene-ethyl acetate-formic acid (36:12:5), is gained a good separation of examined triterpene substances from methanol extracts of inner and external birch bark as well as used standards. From obtained row triterpene mixtures, certain triterpene substances are isolated using method of dry column chromatography. To those substances infrared (IR) spectra were recorded and compared with IR spectra of adequate standards. The study encloses all obtained IR spectra and interpretations on the basis of which can be concluded that triterpene substances, betulin, betulin acid and lupeol isolated from external birch bark give identical characteristic signals and absorbance as referent standards. Method of dry column chromatography has resulted as simple, efficient, repeatable and economical for laboratory conditions. Beside this, a sufficient quantity of examined triterpene substances is also obtained for continuation of their further analytical analysis.
E Kovac-Besović
Elvira
E
Department of Pharmacognosy, Faculty of Pharmacy, University of Sarajevo, Cekalusa 90, 71000 Sarajevo, Bosnia and Herzegovina.
Durić
Kemal
K
Kalodera
Zdenka
Z
Sofić
Emin
E
D000893:Anti-Inflammatory Agents / Q000032:analysis; D000972:Antineoplastic Agents, Phytogenic / Q000032:analysis; D000998:Antiviral Agents / Q000032:analysis; D029662:Betula; D002855:Chromatography, Thin Layer / Q000379:methods; D009828:Oleanolic Acid / Q000032:analysis; D053978:Pentacyclic Triterpenes; D024301:Plant Bark / Q000737:chemistry*; D013055:Spectrophotometry, Infrared / Q000379:methods; D014315:Triterpenes / Q000032:analysis* / Q000302:isolation & purification*; D000094062:Betulinic Acid; D000094042:Lupanes
D000893:Anti-Inflammatory Agents; D000972:Antineoplastic Agents, Phytogenic; D000998:Antiviral Agents; D009828:Oleanolic Acid; D053978:Pentacyclic Triterpenes; D014315:Triterpenes; D000094062:Betulinic Acid; C002503:betulin; C010480:lupeol; D000094042:Lupanes