Human parainfluenza virus type 2 (HPIV2) induced host ADAM8 expression in human salivary adenocarcinoma cell line (HSY) during cell fusion.
BACKGROUND
The aim of the study was to investigate expression of ADAMs (A Disintegrin and A Metalloproteinase) of host cell origin during cell-cell fusion induced by human parainfluenza virus type 2 (HPIV2).


RESULTS
Induction of host cell ADAM9 was observed in GMK cells, but the applicability of this model was restricted by lack of cross-reactivity of the anti-human ADAM8 antibodies with the corresponding green monkey antigens. HSG cells were not susceptible to HPIV2 virus infection. In contrast, in human parotid gland HSY cells, a natural host cell for paramyxoviruses, HPIV2 induced ADAM8 expression. ADAM8 staining increased dramatically over time from 7.9 +/- 3% at zero hours to 99.2 +/- 0.8% at 72 hours (p = 0.0001). Without HPIV2 the corresponding percentages were only 7.7% and 8.8%. Moreover, ADAM8 positive cells formed bi- (16.2%) and multinuclear cells (3.5%) on day one and the corresponding percentages on day three were 15.6% for binuclear and 57.2% for multinuclear cells.


CONCLUSIONS
ADAM8, well recognized for participation in cell-to-cell fusion especially in osteoclast formation, is up-regulated upon formation of multinuclear giant cells after HPIV2 induction in HSY cells. The virus-HSY cell system provides a novel experimental model for study of the molecular mechanism of cell fusion events.
Ma
Guo-Feng
GF
Department of Medicine/invärtes medicin, Helsinki University Central Hospital, PO Box 700, Helsinki, Finland. guofeng.ma@helsinki.fi
Miettinen
Simo
S
Porola
Pauliina
P
Hedman
Klaus
K
Salo
Jari
J
Konttinen
Yrjö T
YT
D051722:ADAM Proteins / Q000378:metabolism*; D000818:Animals; D002459:Cell Fusion; D045744:Cell Line, Tumor; D002522:Chlorocebus aethiops; D015870:Gene Expression; D006801:Humans; D008565:Membrane Proteins / Q000378:metabolism*; D010223:Parainfluenza Virus 2, Human / Q000472:pathogenicity*; D019351:Rubulavirus Infections / Q000378:metabolism*
D008565:Membrane Proteins; D051722:ADAM Proteins; C068490:ADAM8 protein, human