title: Application of retrovirus-mediated expression cloning for receptor screening of a parasite.
abstract: Retrovirus-mediated expression cloning has been applied in both virology and cell biology. Although there is some difficulty in applying this technique to screening for a receptor recognized by an intracellular parasite, we modified the conventional method to identify a putative receptor for the Plasmodium falciparum BAEBL protein. We show that this method is effective in screening for a parasite receptor.
authors.affiliation: Department of Veterinary Microbiology, Graduate School of Agricultural and Life Sciences, University of Tokyo, Bunkyo-ku, Tokyo 113-8657, Japan.
authors.forename: Kyousuke
authors.initials: K
authors.lastname: Kobayashi
authors.forename: Kentaro
authors.initials: K
authors.lastname: Kato
authors.forename: Tatsuki
authors.initials: T
authors.lastname: Sugi
authors.forename: Daisuke
authors.initials: D
authors.lastname: Yamane
authors.forename: Masayuki
authors.initials: M
authors.lastname: Shimojima
authors.forename: Yukinobu
authors.initials: Y
authors.lastname: Tohya
authors.forename: Hiroomi
authors.initials: H
authors.lastname: Akashi
mesh_terms: D000818:Animals; D002352:Carrier Proteins / Q000378:metabolism*; D002460:Cell Line; D003001:Cloning, Molecular / Q000379:methods*; D005434:Flow Cytometry; D053673:Glypicans / Q000302:isolation & purification / Q000378:metabolism*; D006801:Humans; D008565:Membrane Proteins; D008954:Models, Biological; D010963:Plasmodium falciparum / Q000378:metabolism*; D011485:Protein Binding; D015800:Protozoan Proteins / Q000378:metabolism*; D012190:Retroviridae / Q000235:genetics*
chemical_list: D002352:Carrier Proteins; D053673:Glypicans; D008565:Membrane Proteins; D015800:Protozoan Proteins; C451423:erythrocyte binding protein-2, Plasmodium falciparum