Biotransformation of the neonicotinoid insecticides imidacloprid and thiamethoxam by Pseudomonas sp. 1G.
We report the isolation of a Pseudomonas sp. which is able to transform imidacloprid and thiamethoxam under microaerophilic conditions in the presence of an alternate carbon source. This bacterium, Pseudomonas sp. 1G, was isolated from soil with a history of repeated exposure to imidacloprid. Both insecticides were transformed to nitrosoguanidine (NNO), desnitro (NH), and urea (O) metabolites and a transformation pathway is proposed. This is the first conclusive report of bacterial transformation of the 'magic nitro' group which is responsible for the insect selectivity of neonicotinoid insecticides.
Pandey
Gunjan
G
CSIRO Entomology, GPO Box 1700, Canberra, ACT 2601, Australia. Gunjan.Pandey@csiro.au
Dorrian
Susan J
SJ
Russell
Robyn J
RJ
Oakeshott
John G
JG
D001711:Biotransformation; D007093:Imidazoles / Q000378:metabolism*; D007306:Insecticides / Q000378:metabolism*; D000073943:Neonicotinoids; D009574:Nitro Compounds / Q000378:metabolism*; D010078:Oxazines / Q000378:metabolism*; D011549:Pseudomonas / Q000302:isolation & purification / Q000378:metabolism*; D012988:Soil Microbiology; D000077922:Thiamethoxam; D013844:Thiazoles / Q000378:metabolism*
D007093:Imidazoles; D007306:Insecticides; D000073943:Neonicotinoids; D009574:Nitro Compounds; D010078:Oxazines; D013844:Thiazoles; C082359:imidacloprid; D000077922:Thiamethoxam