title: Metabonomics approach to understanding acute and chronic stress in rat models.
abstract: The effects of acute and chronic stress on the production of systemic metabolites were investigated in male Sprague-Dawley (SD) rats. Metabolites excreted in urine were analyzed using GC/MS in conjunction with multivariate and univariate statistical techniques. SD rats were subjected to two kinds of acute stress and chronic unpredictable mild stress, respectively. Metabolic analysis demonstrated that urinary expression of a number of metabolites including glutamate, glutamine, homovanillate, proline, succinate, citrate, and tyrosine altered in the acute stress model in the same way as in the chronic model, while pimelate and hippurate changed in the opposite trend. The results suggested that the stress induced metabolic perturbations were reversible and nonspecific. Metabolic response to chronic combined stress revealed biochemical clues to depression-like symptoms validated by behavior and physiologic results. This study provides a noninvasive and dynamic analytical strategy for the characterization of endogenous metabolic perturbations induced by external stress.
authors.affiliation: Shanghai Center for Systems Biomedicine, and School of Pharmacy, Shanghai Jiao Tong University, Shanghai, 200240, People's Republic of China.
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Wang
authors.forename: Tie
authors.initials: T
authors.lastname: Zhao
authors.forename: Yunping
authors.initials: Y
authors.lastname: Qiu
authors.forename: Mingming
authors.initials: M
authors.lastname: Su
authors.forename: Tao
authors.initials: T
authors.lastname: Jiang
authors.forename: Mingmei
authors.initials: M
authors.lastname: Zhou
authors.forename: Aihua
authors.initials: A
authors.lastname: Zhao
authors.forename: Wei
authors.initials: W
authors.lastname: Jia