title: Identification of Phytophthora fragariae var. rubi by PCR.
abstract: The following chapter describes a PCR method for the identification of the raspberry root rot pathogen Phytophthora fragariae var. rubi. Furthermore, a nested PCR suitable for the detection of the pathogen in infected raspberry roots and validated against the "Duncan bait test" (EPPO Bull 35:87-91, 2005) is explained. Protocols for different DNA extraction methods are given which can be transferred to other fungal pathogens.
authors.affiliation: Plant Health, Scottish Agricultural Science Agency, Edinburgh, UK.
authors.forename: Alexandra
authors.initials: A
authors.lastname: Schlenzig
mesh_terms: D004247:DNA / Q000032:analysis; D004271:DNA, Fungal / Q000235:genetics; D008969:Molecular Sequence Data; D010802:Phylogeny; D010838:Phytophthora / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D010935:Plant Diseases / Q000235:genetics / Q000469:parasitology*; D016133:Polymerase Chain Reaction / Q000379:methods*; D017422:Sequence Analysis, DNA
chemical_list: D004271:DNA, Fungal; D004247:DNA