Preclinical studies of [61Cu]ATSM as a PET radiopharmaceutical for fibrosarcoma imaging.
[61Cu]diacetyl-bis(N4-methylthiosemicarbazone) ([61Cu] ATSM) was prepared using in house-made diacetyl-bis(N4-methylthiosemicarbazone) (ATSM) ligand and [61Cu]CuCl2 produced via the natZn(p, x)61Cu (180 muA proton irradiation, 22 MeV, 3.2 h) and purified by a ion chromatography method. [61Cu]ATSM radiochemical purity was >98 %, as shown by HPLC and RTLC methods. [61Cu]ATSM was administered into normal and tumor bearing rodents for up to 210 minutes, followed by biodistribution and co-incidence imaging studies. Significant tumor/non-tumor accumulation was observed either by animal sacrification or imaging. [61Cu]ATSM is a positron emission tomography (PET) radiotracer for tumor hypoxia imaging.
Jalilian
Amir R
AR
Nuclear Medicine Research Group Agricultural, Medical and Industrial Research School (AMIRS), Karaj, Iran. ajalilian@nrcam.org
Rostampour
Nima
N
Rowshanfarzad
Pejman
P
Shafaii
Kamaleddin
K
Kamali-Dehghan
Mohsen
M
Akhlaghi
Mehdi
M
D000818:Animals; D015687:Cell Hypoxia; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D056831:Coordination Complexes; D003301:Copper Radioisotopes / Q000493:pharmacokinetics; D004353:Drug Evaluation, Preclinical; D005354:Fibrosarcoma / Q000175:diagnosis*; D008297:Male; D009374:Neoplasms, Experimental / Q000175:diagnosis; D009942:Organometallic Compounds / Q000493:pharmacokinetics; D049268:Positron-Emission Tomography / Q000379:methods*; D019275:Radiopharmaceuticals / Q000493:pharmacokinetics; D051381:Rats; D013882:Thiosemicarbazones / Q000493:pharmacokinetics; D014018:Tissue Distribution
D056831:Coordination Complexes; D003301:Copper Radioisotopes; D009942:Organometallic Compounds; D019275:Radiopharmaceuticals; D013882:Thiosemicarbazones; C089548:copper (II) diacetyl-di(N(4)-methylthiosemicarbazone)