Endothelial nitric oxide synthase activation contributes to post-exercise hypotension in spontaneously hypertensive rats.
We investigated the role that endothelial nitric oxide synthase plays in post-exercise hypotension in spontaneously hypertensive rats. To accomplish this, rats were subjected to a single bout of dynamic exercise on a treadmill at 15 m/min for 20 min. L-nitroarginine methyl ester (L-NAME, 40 mg/kg, i.p.) significantly inhibited post-exercise hypotension (25+/-11 and 5+/-3 mm Hg, respectively; P<0.05). In addition, the superoxide anion generation was decreased, while the plasma nitrite production and serine phosphorylation of endothelial nitric oxide synthase were significantly elevated in spontaneously hypertensive rats at 30 min after the termination of exercise. Taken together, these data demonstrate that the increased phosphorylation of endothelial nitric oxide synthase plays a crucial role in the reduction of arterial pressure following a single bout of dynamic exercise in spontaneously hypertensive rats.
Lee
Sang Ki
SK
Infection Signaling Network Research Center, Research Institutes of Biomedical Science, Department of Physiology, College of Medicine, Chungnam National University, Jung-Gu, Daejeon, Republic of Korea.
Kim
Cuk Seong
CS
Kim
Hyo Shin
HS
Cho
Eun Jung
EJ
Joo
Hee Kyoung
HK
Lee
Ji Young
JY
Lee
Eun Ji
EJ
Park
Jin Bong
JB
Jeon
Byeong Hwa
BH
D000818:Animals; D004789:Enzyme Activation; D004791:Enzyme Inhibitors / Q000494:pharmacology; D007022:Hypotension / Q000201:enzymology*; D008297:Male; D019331:NG-Nitroarginine Methyl Ester / Q000494:pharmacology; D052250:Nitric Oxide Synthase Type III / Q000037:antagonists & inhibitors / Q000378:metabolism*; D010766:Phosphorylation; D010805:Physical Conditioning, Animal; D051381:Rats; D011918:Rats, Inbred SHR
D004791:Enzyme Inhibitors; D052250:Nitric Oxide Synthase Type III; D019331:NG-Nitroarginine Methyl Ester