title: Increased ribosomal biogenesis induces pancreatic beta cell failure in mice model of type 2 diabetes.
abstract: OBJECTIVE
  To study the changes in gene expression by pancreatic beta cells under insulin resistance conditions.
  
  
  METHODS
  An exhaustive gene expression analysis was performed, using isolated pancreatic islets of obese diabetic model Lepr(-/-) mice. Overexpression of cyclin D2 was induced in cells from the pancreatic beta cell line, namely, INS-1.
  
  
  RESULTS
  Through a gene expression analysis using islets isolated from db/db mice, we found a significant increase in the expression of ribosome-related molecules. In addition, increased expression of cyclin D2 was found at certain protein levels. As INS-1 cells were induced to overexpress cyclin D2, we found an increase in the expression of ribosome-related molecules. Concurrently, an increase in the expression of endoplasmic reticulum stress (ER stress)-related molecules was also found.
  
  
  CONCLUSIONS
  In cases of pancreatic beta cell hyperplasia associated with insulin resistance, ribosomal biogenesis is increased, and ER stress is induced.
authors.affiliation: Department of Internal Medicine, Division of Diabetes, Metabolism, and Endocrinology, Kobe University Graduate School of Medicine, 7-5-1 Kusunoki-cho, Chuo-ku, Kobe 650-0017, Japan.
authors.forename: Shun-ichiro
authors.initials: S
authors.lastname: Asahara
authors.forename: Tomokazu
authors.initials: T
authors.lastname: Matsuda
authors.forename: Yoshiaki
authors.initials: Y
authors.lastname: Kido
authors.forename: Masato
authors.initials: M
authors.lastname: Kasuga
mesh_terms: D000818:Animals; D003924:Diabetes Mellitus, Type 2 / Q000378:metabolism*; D015870:Gene Expression; D050417:Insulin-Secreting Cells / Q000378:metabolism*; D051379:Mice; D008815:Mice, Inbred Strains; D012270:Ribosomes / Q000235:genetics / Q000378:metabolism*