2-(1-Hydroxethyl)-4,8-dihydrobenzo[1,2-b:5,4-b']dithiophene-4,8-dione (BTP-11) enhances the ATRA-induced differentiation in human leukemia HL-60 cells.
2-(1-Hydroxethyl)-4,8-dihydrobenzo[1,2-b:5,4-b']dithiophene-4,8-dione (BTP-11) is a potent enhancer for all-trans retinoic acid (ATRA)-induced differentiation in HL-60 cells. Combination of BTP-11 and ATRA cut down the concentration of ATRA significantly, and that BTP-11 promoted the progression of ATRA-induced into the terminal granulocytic differentiation. Further, Western blot analysis revealed that combination of BTP-11 and ATRA decreased cyclin D/CDK4 and increased C/EBPvarepsilon protein expression to arrest the cells into G0/G1 phase leading to granulocytic maturation. These results confirmed that BTP-11 is a potent enhancer for ATRA-induced differentiation of HL-60 cells, and the great developmental potential of BTP-11 will be expected.
Chen
Chun-Jen
CJ
Graduate Institute of Pharmaceutical Chemistry, China Medical University, Taichung, Taiwan.
Wen
Yen-Fang
YF
Huang
Pi-Tsan
PT
Hsu
Mei-Hua
MH
Lee
Kuo-Hsiung
KH
Chen
Mei-Hwai
MH
Shin
Wuu-Chian
WC
Huang
Li-Jiau
LJ
Kuo
Sheng-Chu
SC
D015703:Antigens, CD / Q000276:immunology; D015153:Blotting, Western; D002454:Cell Differentiation / Q000187:drug effects*; D004357:Drug Synergism; D005434:Flow Cytometry; D016193:G1 Phase; D018922:HL-60 Cells; D006801:Humans; D016192:Resting Phase, Cell Cycle; D013876:Thiophenes / Q000494:pharmacology*; D014212:Tretinoin / Q000494:pharmacology*
C544856:2-(1-hydroxethyl)-4,8-dihydrobenzo(1,2-b:5,4-b')dithiophene-4,8-dione; D015703:Antigens, CD; D013876:Thiophenes; D014212:Tretinoin