Determination of short-chain fatty acids in dietary fiber extracts using ion-exclusion chromatography with suppressed conductivity detection.
A new chromatographic method for the sequential determination of short-chain fatty acids is described. Acetic, propionic and butyric acids were determined in dietary fiber extracts using ion-exclusion chromatography equipped with inverse chemical suppression and conductivity detection. The best optimization of the chromatographic conditions were achieved when a 100 mm x 7.8 mm ion-exclusion column with a solution of 0.5 mmol L(-1) sulfuric acid as eluent in a flow rate of 0.6 mL min(-1) were employed. The organic acids were sequentially separated in less than 10 min with limits of detection ranging from 1 up to 7.5 micromol L(-1) and limits of quantification from 5 up to 25 micromol L(-1). The linearity of the analytical response was studied in the range of 0.005-10 mmol L(-1) for acetic acid and 0.025-10 mmol L(-1) for propionic and butyric acids with coefficients of determination (R(2)) ranging between 0.9985 and 0.9999. The method was tested and proved to be selective, precise, accurate, reproducible and highly sensitive. Finally, the method was applied in the analysis of biological samples.
Dias
Jailson C
JC
Department of Analytical Chemistry, Institute of Chemistry, University of Campinas UNICAMP, Campinas, SP, Brazil.
Suzuki
Erika
E
de Albuquerque
Cibele L
CL
Ferreira
Anderson L
AL
Brito
Alba R M S
AR
Kubota
Lauro T
LT
D002412:Cations / Q000032:analysis; D002852:Chromatography, Ion Exchange; D004043:Dietary Fiber / Q000032:analysis*; D004553:Electric Conductivity; D005227:Fatty Acids / Q000032:analysis*; D005243:Feces / Q000737:chemistry; D005285:Fermentation; D006801:Humans; D007202:Indicators and Reagents; D015203:Reproducibility of Results
D002412:Cations; D004043:Dietary Fiber; D005227:Fatty Acids; D007202:Indicators and Reagents