Duck (Anas platyrhynchos) linkage mapping by AFLP fingerprinting.
Amplified fragment length polymorphism (AFLP) with multicolored fluorescent molecular markers was used to analyze duck (Anas platyrhynchos) genomic DNA and to construct the first AFLP genetic linkage map. These markers were developed and genotyped in 766 F2 individuals from six families from a cross between two different selected duck lines, brown Tsaiya and Pekin. Two hundred and ninety-six polymorphic bands (64% of all bands) were detected using 18 pairs of fluorescent TaqI/EcoRI primer combinations. Each primer set produced a range of 7 to 29 fragments in the reactions, and generated on average 16.4 polymorphic bands. The AFLP linkage map included 260 co-dominant markers distributed in 32 linkage groups. Twenty-one co-dominant markers were not linked with any other marker. Each linkage group contained three to 63 molecular markers and their size ranged between 19.0 cM and 171.9 cM. This AFLP linkage map provides important information for establishing a duck chromosome map, for mapping quantitative trait loci (QTL mapping) and for breeding applications.
Huang
Chang-Wen
CW
Department of Animal Science, National Chung Hsing University, 250 Kuo-Kung Road, Taichung, Taiwan. amino0116@yahoo.com.tw
Cheng
Yu-Shin
YS
Rouvier
Roger
R
Yang
Kuo-Tai
KT
Wu
Chean-Ping
CP
Huang
Hsiu-Lin
HL
Huang
Mu-Chiou
MC
D054458:Amplified Fragment Length Polymorphism Analysis; D000818:Animals; D001947:Breeding; D002874:Chromosome Mapping; D004372:Ducks / Q000235:genetics*; D005260:Female; D008040:Genetic Linkage; D008297:Male; D011110:Polymorphism, Genetic