title: Characterization and evaluation of a macroporous adsorbent for possible use in the expanded bed adsorption of flavonoids from Ginkgo biloba L.
abstract: The suitability of the use of macroporous adsorbent Amberlite XAD7HP in expanded bed adsorption processes for the isolation of flavonoids from crude extracts of Ginkgo biloba L. has been assessed. The expansion and hydrodynamic properties of expanded beds were investigated and analyzed. The bed expansion as a function of operational fluid velocity was measured and correlated with the Richardson-Zaki equation. Theoretical predictions of the correlation parameters (the terminal settling velocity u(t) and exponent n) were improved by modifying equations in the literature. Residence time distributions (RTDs) were studied using acetone as a tracer. Three measures of liquid phase dispersion (the height equivalent of theoretical plate, Bodenstein number and axial distribution coefficient) were investigated and compared to values previously obtained with commercial EBA adsorbents developed for protein purification. A suitable bed expansion ratio was found to be 1.25 times the settled bed height, which occurred at a corresponding flow velocity of 183 cm/h. For an initial settled bed height of 42 cm, the mean residence time of liquid in the expanded bed was around 28 min. Under these flow conditions, the axial mixing coefficient D(ax) was 7.54 x 10(-6) m(2)/s and the Bodenstein number was 28; the number of theoretical plates (N) was 19 and the height equivalent of a theoretical plate (HETP) was 2.77 cm. Rutin trihydrate was used as a model flavonoid for the characterization of the adsorption properties of Amberlite XAD7HP. Adsorption was observed to reach equilibrium within 3 h with 70% of the adsorption capacity being achieved within 30 min. The estimated maximum equilibrium adsorption capacity for rutin was estimated to be 43.0 mg/(gresin) when the results were fitted to Langmuir isotherms. The adsorption performance was not seriously impaired by the physical presence of G. biloba leaf powders. Assessment of the kinetics of the adsorption of rutin revealed that the rate constant for adsorption was only reduced by 15% in the presence of leaf powders at a concentration of 50 mg/mL. The results demonstrated that Amberlite XAD7HP should be suitable for expanded bed adsorption of flavonoids from crude extracts of G. biloba L.
authors.affiliation: Department of Chemical Engineering and Biotechnology, University of Cambridge, Pembroke Street, Cambridge CB23RA, UK. jl426@cam.ac.uk
authors.forename: Jing
authors.initials: J
authors.lastname: Li
authors.forename: Howard A
authors.initials: HA
authors.lastname: Chase
mesh_terms: D000180:Acrylic Resins / Q000737:chemistry*; D000327:Adsorption; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005419:Flavonoids / Q000737:chemistry / Q000302:isolation & purification*; D020441:Ginkgo biloba / Q000737:chemistry*; D007700:Kinetics; D018515:Plant Leaves / Q000737:chemistry; D011137:Polystyrenes / Q000737:chemistry*; D016062:Porosity; D011208:Powders; D012212:Rheology; D012431:Rutin / Q000302:isolation & purification; D013696:Temperature; D013997:Time Factors
chemical_list: D000180:Acrylic Resins; D005419:Flavonoids; D011137:Polystyrenes; D011208:Powders; C015661:amberlite XAD 7; D012431:Rutin