Mouse x pig chimeric antibodies expressed in Baculovirus retain the same properties of their parent antibodies.
The development of hybridoma and recombinant DNA technologies has made it possible to use antibodies against cancer, autoimmune disorders, and infectious diseases in humans. These advances in therapy, as well as immunoprophylaxis, could also make it possible to use these technologies in agricultural species of economic importance such as pigs. Porcine reproductive and respiratory syndrome virus (PRRSV) is an arterivirus causing very important economic losses to the industry. Passive transfer of antibodies obtained by biotechnology could be used in the future to complement or replace vaccination against this and other pig pathogens. To this end, we constructed and studied the properties of chimeric mouse x pig anti-PRRSV antibodies. We cloned the constant regions of gamma-1 and gamma-2 heavy chains and the lambda light chain of pig antibodies in frame with the variable regions of heavy and light chains of mouse monoclonal antibody ISU25C1, which has neutralizing activity against PRRSV. The coding regions for chimeric IgG1 and IgG2 were expressed in a baculovirus expression system. Both chimeric antibodies recognized PRRSV in ELISA as well as in a Western-blot format and, more importantly, were able to neutralize PRRSV in the same fashion as the parent mouse monoclonal antibody ISU25C1. In addition, we show that both pig IgG1 and IgG2 antibodies could bind complement component C1q, with IgG2 being more efficient than IgG1 in binding C1q. Expressing chimeric pig antibodies with protective capabilities offers a new alternative strategy for infectious disease control in domestic pigs.
Jar
Ana M
AM
Veterinary and Biomedical Sciences Dept., University of Nebraska-Lincoln, Lincoln, NE 68583, USA.
Osorio
Fernando A
FA
López
Osvaldo J
OJ
D000818:Animals; D000906:Antibodies / Q000235:genetics* / Q000276:immunology* / Q000627:therapeutic use; D016367:Baculoviridae / Q000235:genetics* / Q000378:metabolism; D015870:Gene Expression; D007074:Immunoglobulin G / Q000235:genetics / Q000276:immunology / Q000627:therapeutic use; D051379:Mice; D019318:Porcine Reproductive and Respiratory Syndrome / Q000188:drug therapy / Q000276:immunology; D019316:Porcine respiratory and reproductive syndrome virus / Q000235:genetics / Q000276:immunology*; D015202:Protein Engineering / Q000379:methods*; D013552:Swine
D000906:Antibodies; D007074:Immunoglobulin G