title: The effect of trans-10, cis-12 conjugated linoleic acid on gene expression profiles related to lipid metabolism in human intestinal-like Caco-2 cells.
abstract: We conducted an in-depth investigation of the effects of conjugated linoleic acid (CLA) on the expression of key metabolic genes and genes of known importance in intestinal lipid metabolism using the Caco-2 cell model. Cells were treated with 80 mumol/L of linoleic acid (control), trans-10, cis-12 CLA or cis-9, trans-11 CLA. RNA was isolated from the cells, labelled and hybridized to the Affymetrix U133 2.0 Plus arrays (n = 3). Data and functional analysis were preformed using Bioconductor. Gene ontology analysis (GO) revealed a significant enrichment (P < 0.0001) for the GO term lipid metabolism with genes up-regulated by trans-10, cis-12 CLA. Trans-10, cis-12 CLA, but not cis-9, trans-11 CLA, altered the expression of a number of genes involved in lipid transport, fatty acid metabolism, lipolysis, beta-oxidation, steroid metabolism, cholesterol biosynthesis, membrane lipid metabolism, gluconeogenesis and the citrate cycle. These observations warrant further investigation to understand their potential role in the metabolic syndrome.
authors.affiliation: Department of Food and Nutritional Sciences, University College Cork, Cork, Ireland, Eileen.Murphy@ucc.ie.
authors.forename: Eileen F
authors.initials: EF
authors.lastname: Murphy
authors.forename: Guido J
authors.initials: GJ
authors.lastname: Hooiveld
authors.forename: Michael
authors.initials: M
authors.lastname: Müller
authors.forename: Raffaelle A
authors.initials: RA
authors.lastname: Calogero
authors.forename: Kevin D
authors.initials: KD
authors.lastname: Cashman