Overexpression of E2F-1 inhibits progression of gastric cancer in vitro.
E2F-1 plays a critical role in cell cycle regulation and other biological processes in cells. E2F-1 mediates apoptosis and suppresses tumorigenesis in many tissue types, but there are few data available on E2F-1 expression and its relationship to tumor kinetics in gastric cancer. To gain better insight into the involvement of E2F-1 in the biological characteristics of gastric tumors, we investigated the effect of E2F-1 overexpression on the progression of gastric carcinoma cells. A gastric cancer cell line stably overexpressing E2F-1 (MGC-803/E2F-1) was established. The influence of E2F-1 overexpression on in vitro cell growth was assessed by measuring cell survival, colony formation, and cell cycle progression. The results clearly show that overexpression of E2F-1 significantly inhibits cell growth and proliferation, blocking entry into the S-phase of the cell cycle. MGC-803/E2F-1 cells also had a higher apoptotic rate than control cells. In addition, E2F-1 reduced the motility and invasion of gastric cancer cells.
Xie
Yubo
Y
Department of Surgery, The First Affiliated Hospital, Guangxi Medical University, Guangxi, PR China.
Wang
Changqing
C
Li
Lei
L
Ma
Yulin
Y
Yin
Yongshuo
Y
Xiao
Qiang
Q
D017209:Apoptosis; D002453:Cell Cycle; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D050687:E2F1 Transcription Factor / Q000378:metabolism*; D006801:Humans; D013274:Stomach Neoplasms / Q000378:metabolism* / Q000473:pathology; D014162:Transfection
D050687:E2F1 Transcription Factor; C493753:E2F1 protein, human