title: Initial hepatosplanchnic blood flow distribution and oxygen metabolism in experimental model of hypotensive brain death.
abstract: BACKGROUND
  Organs from the so-called marginal donors have been used with a significant higher risk of primary non function than organs retrieved from the optimal donors. We investigated the early metabolic changes and blood flow redistribution in splanchnic territory in an experimental model that mimics marginal brain-dead (BD) donor.
  
  
  METHODS
  Ten dogs (21.3+/-0.9 kg), were subjected to a brain death protocol induced by subdural balloon inflation and observed for 30 min thereafter without any additional interventions. Mean arterial and intracranial pressures, heart rate, cardiac output (CO), portal vein and hepatic artery blood flows (PVBF and HABF, ultrasonic flowprobe), and O(2)-derived variables were evaluated.
  
  
  RESULTS
  An increase in arterial pressure, CO, PVBF and HABF was observed after BD induction. At the end, an intense hypotension with normalization in CO (3.0+/-0.2 vs. 2.8+/-2.8 L/min) and PVBF (687+/-114 vs. 623+/-130 ml/min) was observed, whereas HABF (277+/-33 vs. 134+/-28 ml/min, p<0.005) remained lower than baseline values.
  
  
  CONCLUSIONS
  Despite severe hypotension induced by sudden increase of intracranial pressure, the systemic and splanchnic blood flows were partially preserved without signs of severe hypoperfusion (i.e. hyperlactatemia). Additionally, the HABF was mostly negatively affected in this model of marginal BD donor. Our data suggest that not only the cardiac output, but the intrinsic hepatic microcirculatory mechanism plays a role in the hepatic blood flow control after BD.
authors.affiliation: Division of Applied Physiology, Heart Institute (InCor), University of Sao Paulo School of Medicine, Sao Paulo, Brazil.
authors.forename: Fabio A
authors.initials: FA
authors.lastname: De Luca
authors.forename: Ruy J
authors.initials: RJ
authors.lastname: Cruz
authors.forename: Alejandra Del Pilar Gallardo
authors.initials: Adel P
authors.lastname: Garrido
authors.forename: Ricardo
authors.initials: R
authors.lastname: Prist
authors.forename: Mauricio
authors.initials: M
authors.lastname: Rocha-E-Silva
mesh_terms: D000818:Animals; D001926:Brain Death / Q000378:metabolism / Q000503:physiopathology*; D002302:Cardiac Output / Q000502:physiology; D004195:Disease Models, Animal; D004285:Dogs; D006439:Hemodynamics; D008102:Liver Circulation / Q000502:physiology; D008833:Microcirculation; D010100:Oxygen / Q000097:blood*; D011169:Portal Vein / Q000503:physiopathology; D012039:Regional Blood Flow; D013152:Splanchnic Circulation / Q000502:physiology*
chemical_list: D010100:Oxygen