Untargeted LC-Q-TOF mass spectrometry method for the detection of adulterations in skimmed-milk powder.
A nontargeted protein identification method was developed to screen for adulterations in skimmed-milk powder (SMP). There are indications of falsified SMP content due to the addition of plant proteins. To demonstrate the reliability and accuracy of the developed comparative LC-MS method using a quadrupole TOF MS instrument, adulterated SMP samples were prepared by the addition of protein isolates of soy and pea to skimmed-milk before pasteurisation and evaporation. The comparative LC-MS approach enabled unequivocal discrimination of those SMP samples containing soy and pea protein from nonadulterated SMP. To identify the source of (plant) proteins present in the adulterated SMP, data-dependent LC-MS/MS was used in combination with an include list of differential peptides. Numerous peptides originating from the major seed proteins of soy (glycinin, beta-conglycin) and pea (legumin, vicilin) could be identified in this way.
Cordewener
Jan H G
JH
PRI - Plant ResearchInternational, Wageningen UR, The Netherlands. jan.cordewener@wur.nl
Luykx
Dion M A M
DM
Frankhuizen
Rob
R
Bremer
Maria G E G
MG
Hooijerink
Hendricus
H
America
Antoine H P
AH
D000818:Animals; D002853:Chromatography, Liquid / Q000295:instrumentation / Q000379:methods*; D005504:Food Analysis / Q000295:instrumentation / Q000379:methods; D005506:Food Contamination / Q000032:analysis*; D013058:Mass Spectrometry / Q000295:instrumentation / Q000379:methods*; D008892:Milk / Q000737:chemistry*; D008969:Molecular Sequence Data; D018532:Pisum sativum / Q000737:chemistry; D010455:Peptides / Q000032:analysis* / Q000235:genetics; D010940:Plant Proteins / Q000032:analysis / Q000235:genetics; D011208:Powders / Q000737:chemistry*; D013025:Glycine max / Q000737:chemistry
D010455:Peptides; D010940:Plant Proteins; D011208:Powders