Isolation of dense core secretory vesicles from pancreatic endocrine cells by differential and density gradient centrifugation.
Methods are presented for the separation of dense core secretory vesicles from insulin-secreting tissues (insulin granules) based on a combination of differential and density gradient centrifugation on various media. Emphasis is given to the use of transplantable tumors, tissue culture cell lines, and pancreatic islets as a tissue source.
Hutton
John C
JC
Barbara Davis Center for Childhood Diabetes, University of Colorado at Denver and Health Sciences Center, Aurora, Colorado, USA.
Wong
Randall
R
Davidson
Howard W
HW
D000818:Animals; D002458:Cell Fractionation / Q000379:methods*; D002460:Cell Line; D002499:Centrifugation, Density Gradient; D050417:Insulin-Secreting Cells / Q000648:ultrastructure*; D007340:Insulinoma / Q000648:ultrastructure; D007472:Iohexol; D007515:Islets of Langerhans / Q000648:ultrastructure; D009374:Neoplasms, Experimental / Q000473:pathology; D010190:Pancreatic Neoplasms / Q000648:ultrastructure; D011205:Povidone; D051381:Rats; D022142:Secretory Vesicles; D012822:Silicon Dioxide
D007472:Iohexol; C016039:Percoll; D012822:Silicon Dioxide; D011205:Povidone