title: Regional variation in adipogenesis and IGF regulatory proteins in the fetal baboon.
abstract: Intrauterine growth rate is associated with body distribution in adulthood suggesting differential response of fetal fat depots to nutritional modifications. We hypothesize that there is regional differences in fetal adipogenesis, in part, due to depot-specific regulation of the availability of insulin growth factors. In near-term baboon fetuses (n=3-5), the subcutaneous abdominal vs. omental preadipocytes had (1) more extensive lipid accumulation as assessed by BODIPY (lipid staining) to DAPI (nuclei) absorbance ratios (mean+/-SEM; 0.51+/-0.21, 0.35+/-0.09, p<0.05), (2) lower (p<0.05) secretion of IGF-binding protein 4 (9.6+/-1.2 vs. 17.4+/-2.8 ng/ml) and its protease pregnancy associated plasma protein A (24.6+/-1.9 vs. 39.1+/-6.3 microIU/ml), (3) lower protein expression of IGF2 "clearance" receptor in cell lysate (0.28+/-0.03 vs. 0.53+/-0.02 OD U/mm(2), p<0.05); all variables were intermediate in femoral preadipocytes. The regional variation of the adipogenesis and the IGF regulatory pathway set the stage for differential responsiveness of fat depots to external signals.
authors.affiliation: Pennington Biomedical Research Center, Department of Human Physiology, 6400 Perkins Road, Baton Rouge, LA 70808, USA. yourka.tchoukalova@pbrc.edu
authors.forename: Yourka D
authors.initials: YD
authors.lastname: Tchoukalova
authors.forename: Peter W
authors.initials: PW
authors.lastname: Nathanielsz
authors.forename: Cheryl A
authors.initials: CA
authors.lastname: Conover
authors.forename: Steven R
authors.initials: SR
authors.lastname: Smith
authors.forename: Eric
authors.initials: E
authors.lastname: Ravussin
mesh_terms: D017667:Adipocytes / Q000166:cytology / Q000502:physiology*; D050156:Adipogenesis; D000818:Animals; D002478:Cells, Cultured; D005260:Female; D005333:Fetus / Q000166:cytology / Q000502:physiology*; D018974:Insulin-Like Growth Factor Binding Protein 4 / Q000378:metabolism; D010215:Papio; D011247:Pregnancy; D011266:Pregnancy-Associated Plasma Protein-A / Q000378:metabolism; D017451:Receptors, Somatomedin / Q000378:metabolism
chemical_list: D018974:Insulin-Like Growth Factor Binding Protein 4; D017451:Receptors, Somatomedin; D011266:Pregnancy-Associated Plasma Protein-A