title: Plasmonic nanoholes in a multichannel microarray format for parallel kinetic assays and differential sensing.
abstract: We present nanohole arrays in a gold film integrated with a six-channel microfluidic chip for parallel measurements of molecular binding kinetics. Surface plasmon resonance effects in the nanohole arrays enable real-time, label-free measurements of molecular binding events in each channel, while adjacent negative reference channels can record measurement artifacts such as bulk solution index changes, temperature variations, or changing light absorption in the liquid. With the use of this platform, streptavidin-biotin specific binding kinetics are measured at various concentrations with negative controls. A high-density microarray of 252 biosensing pixels is also demonstrated with a packing density of 10(6) sensing elements/cm(2), which can potentially be coupled with a massively parallel array of microfluidic channels for protein microarray applications.
authors.affiliation: Laboratory of Nanostructures and Biosensing, Department of Electrical and Computer Engineering, University of Minnesota, Twin Cities, 200 Union Street South East, Minneapolis, Minnesota 55455, USA.
authors.forename: Hyungsoon
authors.initials: H
authors.lastname: Im
authors.forename: Antoine
authors.initials: A
authors.lastname: Lesuffleur
authors.forename: Nathan C
authors.initials: NC
authors.lastname: Lindquist
authors.forename: Sang-Hyun
authors.initials: SH
authors.lastname: Oh
mesh_terms: D001710:Biotin / Q000378:metabolism; D003198:Computer Simulation; D007267:Injections; D007700:Kinetics; D007834:Lasers; D046228:Microarray Analysis / Q000295:instrumentation*; D046210:Microfluidic Analytical Techniques; D008958:Models, Molecular; D036103:Nanotechnology / Q000295:instrumentation*; D019809:Streptavidin / Q000378:metabolism; D013382:Subtraction Technique; D020349:Surface Plasmon Resonance / Q000295:instrumentation*; D013696:Temperature
chemical_list: D001710:Biotin; D019809:Streptavidin