PCR detection of potato cyst nematode.
Potato cyst nematode (PCN) is responsible for losses in potato production totalling millions of euros every year in the EC. It is important for growers to know which species is present in their land as this determines its subsequent use. The two species Globodera pallida and Globodera rostochiensis can be differentiated using an allele-specific PCR.
Reid
Alex
A
DMB, Scottish Agricultural Science Agency, Roddinglaw Road, Edinburgh, UK.
D000818:Animals; D019411:Clinical Laboratory Techniques; D021903:DNA, Ribosomal Spacer / Q000032:analysis / Q000235:genetics; D009348:Nematoda / Q000235:genetics / Q000302:isolation & purification*; D010935:Plant Diseases / Q000469:parasitology*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012336:RNA, Ribosomal, 16S / Q000032:analysis / Q000235:genetics; D012680:Sensitivity and Specificity; D011198:Solanum tuberosum / Q000469:parasitology*
D021903:DNA, Ribosomal Spacer; D012336:RNA, Ribosomal, 16S