Chitosan based oligoamine polymers: synthesis, characterization, and gene delivery.
A series of chitosan-based oligoamine polymers was synthesized from N-maleated chitosan (NMC) via Michael addition with diethylenetriamine (DETA), triethylenetetramine (TETA), tetraethylenepentamine (TEPA) and linear polyethylenimine (M(n) 423), respectively. The resulted polymers exhibited well binding ability to condense plasmid DNA to form complexes with size ranging from 200 to 600 nm when the polymer/DNA weight ratio was above 7. The polymer/DNA complexes observed by scanning electron microscopy (SEM) exhibited a compact and spherical morphology. The cytotoxicity assay showed that the synthesized polymers were less toxic than that of PEI(25 K). The gene transfection effect of resulted polymers was evaluated in 293T and HeLa cells, and the results showed that the gene transfection efficiency of these polymers was better than that of chitosan. Moreover, the transfection efficiency was dependent on the length of the oligoamine side chains and the molecular weight of the chitosan derivatives.
Lu
Bo
B
Key Laboratory of Biomedical Polymers of Ministry of Education, Department of Chemistry, Wuhan University, Wuhan 430072, PR China.
Wang
Chang-Fang
CF
Wu
De-Qun
DQ
Li
Cao
C
Zhang
Xian-Zheng
XZ
Zhuo
Ren-Xi
RX
D002460:Cell Line; D048271:Chitosan / Q000737:chemistry*; D004247:DNA / Q000378:metabolism; D005029:Ethylenediamines / Q000138:chemical synthesis / Q000737:chemistry; D018014:Gene Transfer Techniques; D005822:Genetic Vectors / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D007668:Kidney / Q000166:cytology; D008970:Molecular Weight; D010957:Plasmids / Q000235:genetics; D011073:Polyamines / Q000138:chemical synthesis / Q000737:chemistry; D011094:Polyethyleneimine / Q000737:chemistry; D011108:Polymers / Q000138:chemical synthesis* / Q000737:chemistry*; D017433:Protein Structure, Secondary; D014162:Transfection; D014266:Trientine / Q000138:chemical synthesis / Q000737:chemistry
D005029:Ethylenediamines; D011073:Polyamines; D011108:Polymers; C005391:diethylenetriamine; D011094:Polyethyleneimine; D004247:DNA; D048271:Chitosan; D014266:Trientine; C034269:tetraethylenepentamine