DNA typing using AmpFlSTR Y-filer for very long-term stored specimens.
Aware that long-term storage bloodstain generally reduces numbers of detectable loci, we performed DNA typing with bloodstains stored at room temperature for 22-30years, then extracted DNA from the bloodstains using the AmpFlSTR Y-filer PCR Amplification Kit. We performed electrophoresis with an ABI 310 Genetic Analyzer and determined alleles using GeneMapper ID v3.2 software. Our results suggest that Y-filer finds certain loci more difficult to anneal than others.
Tsukada
Kazuhiko
K
Department of Forensic Medicine, Criminal Investigation Laboratory, Nagano Pref. Police H.Q., 3916 Nishijo, Matsushiro-Machi, Nagano-shi, Nagano 381-1232, Japan. tuk-lab@mx1.avis.ne.jp
Shimizu
Maiko
M
Kurasawa
Yoshinobu
Y
Kasahara
Koichi
K
D001801:Blood Stains; D041322:Chromosomes, Human, Y; D004247:DNA / Q000302:isolation & purification*; D053836:DNA Degradation, Necrotic; D016172:DNA Fingerprinting; D004587:Electrophoresis, Agar Gel; D006801:Humans; D008297:Male; D016133:Polymerase Chain Reaction; D013048:Specimen Handling; D013997:Time Factors
D004247:DNA