title: Regulation of tyrosine phosphatases in the adventitia during vascular remodelling.
abstract: Protein tyrosine phosphatases (PTPs) are regulators of growth factor signalling in vascular remodelling. The aim of this study was to evaluate PTP expression in the context of PDGF-signalling in the adventitia after angioplasty. Utilising a rat carotid artery model, the adventitial layers of injured and non-injured vessels were laser microdissected. The mRNA expression of the PDGF beta-receptor, the ligands PDGF-A/B/C/D and the receptor-antagonising PTPs (DEP-1, TC-PTP, SHP-2, PTP1B) were determined and correlated to vascular morphometrics, proliferation markers and PDGF beta-receptor phosphorylation. The levels of the PDGF beta-receptor, PDGF-C and PDGF-D were upregulated concurrently with the antagonising PTPs DEP-1 and TC-PTP at day 8, and normalised at day 14 after vessel injury. Although the proliferation parameters were time-dependently altered in the adventitial layer, the phosphorylation of the PDGF beta-receptor remained unchanged. The expression dynamics of specific PTPs indicate a regulatory role of PDGF-signalling also in the adventitia during vascular remodelling.
authors.affiliation: Cancer Centrum Karolinska, Department of Oncology-Pathology, Karolinska Institute, Stockholm, Sweden.
authors.forename: Patrick
authors.initials: P
authors.lastname: Micke
authors.forename: Daniel
authors.initials: D
authors.lastname: Hackbusch
authors.forename: Sibel
authors.initials: S
authors.lastname: Mercan
authors.forename: Philipp
authors.initials: P
authors.lastname: Stawowy
authors.forename: Oleg
authors.initials: O
authors.lastname: Tsuprykov
authors.forename: Thomas
authors.initials: T
authors.lastname: Unger
authors.forename: Arne
authors.initials: A
authors.lastname: Ostman
authors.forename: Kai
authors.initials: K
authors.lastname: Kappert
mesh_terms: D000818:Animals; D002339:Carotid Arteries / Q000378:metabolism / Q000503:physiopathology*; D020212:Carotid Artery Injuries / Q000378:metabolism / Q000503:physiopathology*; D049109:Cell Proliferation; D003238:Connective Tissue / Q000201:enzymology*; D004730:Endothelium, Vascular / Q000378:metabolism / Q000503:physiopathology; D020869:Gene Expression Profiling; D023421:Models, Animal; D010766:Phosphorylation; D010982:Platelet-Derived Growth Factor / Q000096:biosynthesis; D017027:Protein Tyrosine Phosphatases / Q000096:biosynthesis*; D051381:Rats; D017207:Rats, Sprague-Dawley; D020797:Receptor, Platelet-Derived Growth Factor beta / Q000096:biosynthesis / Q000378:metabolism*; D012038:Regeneration; D015398:Signal Transduction
chemical_list: D010982:Platelet-Derived Growth Factor; D020797:Receptor, Platelet-Derived Growth Factor beta; D017027:Protein Tyrosine Phosphatases