[Somatic mosaicism in mice detected using the RAPD-PCR method].
99 DNA samples of organs and tissues of 18 mice were examined using the method of PCR amplification with random primers. Among 27 oligonucleotide primers tested, 4 producing stable, well-reproducible profiles of amplification products were chosen for further analysis. Using 2 of these primers we detected differences in RAPD-profiles in some tissues in several individuals. These differences were associated with the modification of mobility, or with the fragment gain/loss in the RAPD profile, and could be caused either by genomic rearrangements, or mutations involving the regions of the DNA-primer pairing. Different epigenetic factors may also contribute to this process.
Butovskaia
P R
PR
Pavlova
G V
GV
Martirosian
I A
IA
Sukhikh
G T
GT
Ryskov
A P
AP
D000818:Animals; D017931:DNA Primers / Q000235:genetics; D015321:Gene Rearrangement / Q000502:physiology*; D016678:Genome / Q000502:physiology*; D051379:Mice; D008808:Mice, Inbred CBA; D009030:Mosaicism; D009928:Organ Specificity / Q000502:physiology; D019105:Random Amplified Polymorphic DNA Technique; D013045:Species Specificity
D017931:DNA Primers