title: Duck (Anas platyrhynchos) linkage mapping by AFLP fingerprinting.
abstract: Amplified fragment length polymorphism (AFLP) with multicolored fluorescent molecular markers was used to analyze duck (Anas platyrhynchos) genomic DNA and to construct the first AFLP genetic linkage map. These markers were developed and genotyped in 766 F2 individuals from six families from a cross between two different selected duck lines, brown Tsaiya and Pekin. Two hundred and ninety-six polymorphic bands (64% of all bands) were detected using 18 pairs of fluorescent TaqI/EcoRI primer combinations. Each primer set produced a range of 7 to 29 fragments in the reactions, and generated on average 16.4 polymorphic bands. The AFLP linkage map included 260 co-dominant markers distributed in 32 linkage groups. Twenty-one co-dominant markers were not linked with any other marker. Each linkage group contained three to 63 molecular markers and their size ranged between 19.0 cM and 171.9 cM. This AFLP linkage map provides important information for establishing a duck chromosome map, for mapping quantitative trait loci (QTL mapping) and for breeding applications.
authors.affiliation: Department of Animal Science, National Chung Hsing University, 250 Kuo-Kung Road, Taichung, Taiwan. amino0116@yahoo.com.tw
authors.forename: Chang-Wen
authors.initials: CW
authors.lastname: Huang
authors.forename: Yu-Shin
authors.initials: YS
authors.lastname: Cheng
authors.forename: Roger
authors.initials: R
authors.lastname: Rouvier
authors.forename: Kuo-Tai
authors.initials: KT
authors.lastname: Yang
authors.forename: Chean-Ping
authors.initials: CP
authors.lastname: Wu
authors.forename: Hsiu-Lin
authors.initials: HL
authors.lastname: Huang
authors.forename: Mu-Chiou
authors.initials: MC
authors.lastname: Huang
mesh_terms: D054458:Amplified Fragment Length Polymorphism Analysis; D000818:Animals; D001947:Breeding; D002874:Chromosome Mapping; D004372:Ducks / Q000235:genetics*; D005260:Female; D008040:Genetic Linkage; D008297:Male; D011110:Polymorphism, Genetic