In vivo analysis of dendritic cell development and homeostasis.
Dendritic cells (DCs) in lymphoid tissue arise from precursors that also produce monocytes and plasmacytoid DCs (pDCs). Where DC and monocyte lineage commitment occurs and the nature of the DC precursor that migrates from the bone marrow to peripheral lymphoid organs are unknown. We show that DC development progresses from the macrophage and DC precursor to common DC precursors that give rise to pDCs and classical spleen DCs (cDCs), but not monocytes, and finally to committed precursors of cDCs (pre-cDCs). Pre-cDCs enter lymph nodes through and migrate along high endothelial venules and later disperse and integrate into the DC network. Further cDC development involves cell division, which is controlled in part by regulatory T cells and fms-like tyrosine kinase receptor-3.
Liu
Kang
K
Laboratory of Molecular Immunology, Rockefeller University, New York, NY 10065, USA. liuk@rockefeller.edu
Victora
Gabriel D
GD
Schwickert
Tanja A
TA
Guermonprez
Pierre
P
Meredith
Matthew M
MM
Yao
Kaihui
K
Chu
Fei-Fan
FF
Randolph
Gwendalyn J
GJ
Rudensky
Alexander Y
AY
Nussenzweig
Michel
M
D019264:Adoptive Transfer; D000818:Animals; D001808:Blood Vessels / Q000166:cytology; D001854:Bone Marrow Cells / Q000166:cytology; D002454:Cell Differentiation; D002455:Cell Division; D019070:Cell Lineage; D002465:Cell Movement; D048430:Cell Shape; D003713:Dendritic Cells / Q000166:cytology* / Q000276:immunology / Q000502:physiology; D006706:Homeostasis; D008198:Lymph Nodes / Q000098:blood supply / Q000166:cytology / Q000276:immunology; D008221:Lymphoid Tissue / Q000098:blood supply / Q000166:cytology* / Q000276:immunology; D008264:Macrophages / Q000166:cytology; D051379:Mice; D009000:Monocytes / Q000166:cytology*; D023461:Myeloid Progenitor Cells / Q000166:cytology* / Q000502:physiology; D010227:Parabiosis; D015398:Signal Transduction; D013154:Spleen / Q000166:cytology / Q000276:immunology; D050378:T-Lymphocytes, Regulatory / Q000502:physiology; D014699:Venules / Q000166:cytology; D051941:fms-Like Tyrosine Kinase 3 / Q000235:genetics / Q000378:metabolism
C497971:Flt3 protein, mouse; D051941:fms-Like Tyrosine Kinase 3