Real-time PCR method for identification of Asian populations in forensic casework.
The priorities for mass disaster victim identification are rapid investigation turn-around time and low cost. We describe a DNA typing procedure to selectively identify members of Asian populations by a real-time PCR method using polymorphisms of the alpha2 chain of the type I collagen gene (COL1A2) and mitochondrial DNA (mtDNA). Among the 50 members of the Asian population included in the present study, 37 harbored a deleted allele in intron 33 of COL1A2 (26822-26823del) or the 10400C>T substitution mutation in mtDNA to give a probability of 0.740 for these SNPs in the Asian population.
Tokutomi
Tomoharu
T
Department of Forensic Medicine, National Defense Medical College, 3-2 Namiki, Tokorozawa, Saitama 359-8513, Japan. dr20002@ndmc.ac.jp
Takada
Yuzo
Y
Murayama
Takako
T
Mukaida
Masahiro
M
Kanetake
Jun
J
D044466:Asian People / Q000235:genetics*; D003094:Collagen / Q000235:genetics*; D024042:Collagen Type I; D016172:DNA Fingerprinting / Q000379:methods*; D017931:DNA Primers; D004272:DNA, Mitochondrial / Q000235:genetics*; D005828:Genetics, Population; D006801:Humans; D007438:Introns; D007564:Japan; D009154:Mutation; D016133:Polymerase Chain Reaction / Q000379:methods; D020641:Polymorphism, Single Nucleotide
D024042:Collagen Type I; D017931:DNA Primers; D004272:DNA, Mitochondrial; D003094:Collagen