Diagnostic and analytical mutation scanning of Cryptosporidium: utility and advantages.
Cryptosporidiosis is predominantly a disease of the alimentary tract of humans and other vertebrates, caused by parasitic protists of the genus Cryptosporidium. This disease, transmitted mainly via the fecal-oral route (in water or food), is of major socioeconomic importance globally. The diagnosis of cryptosporidiosis, including the genetic characterization of the different species, genotypes and subgenotypes (population variants) of Cryptosporidium, is crucial to prevention and control, particularly as there is no cost-effective treatment against this disease. Although traditional phenetic techniques have had major deficiencies for the specific diagnosis of cryptosporidiosis, there has been substantial progress in the establishment of molecular tools. In this article, we review key genetic markers used for the specific identification of Cryptosporidium, diagnosis of cryptosporidiosis and analysis of genetic variation in Cryptosporidium populations. We also discuss the advantages and disadvantages of selected techniques, and emphasize the benefits of utilizing rapid mutation scanning in achieving improved insights into the population genetics and epidemiology of Cryptosporidium.
Jex
Aaron R
AR
Department of Veterinary Science, The University of Melbourne, Werribee, Victoria 3030, Australia. ajex@unimelb.edu.au
Gasser
Robin B
RB
D000818:Animals; D003457:Cryptosporidiosis / Q000175:diagnosis* / Q000453:epidemiology / Q000235:genetics* / Q000517:prevention & control; D003458:Cryptosporidium / Q000235:genetics*; D004252:DNA Mutational Analysis / Q000379:methods; D005819:Genetic Markers; D006801:Humans; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D009154:Mutation; D013045:Species Specificity
D005819:Genetic Markers