title: Calli cultures from Abies equi-trojani (Aschers et Sinten) and changes in antioxidant defense system enzymes.
abstract: This study was conducted to explain difficulties of indirect regeneration of forest trees in tissue culture conditions. For this purpose, changes of antioxidant defense system enzymes; superoxide dismutase (SOD), peroxidase (POD) activities were determined during calli formation on young apical shoots of Abies equi-trojani (Aschers et Sinten). Young apical shoots were collected from naturally growing trees and cultured on two different media; Murashige and Skoog (MS) and McCown Woody plant medium (WPM) supplemented with growth regulators benzyl amino purine (BAP), 2,4-dichloro phenoxy acetic acid (2.4-D), kinetin (Kn) in various concentrations for callus induction. WPM media containing 1 mg ml(-1) BAP and 1 mg ml(-1) 2,4-D gave the best calli induction ratio (74%) between tested combinations. POD and SOD enzyme activities were measured both on young shoot explants and 10 day-old calli derived from these explants. POD and SOD enzyme activities were higher being 81.02% and 74.82%, respectively on calli when compared to shoots. The results showed that culture stress tolerated with increased antioxidant enzyme activities could be considered as protective physiological responses in calli cells.
authors.affiliation: Department of Molecular Biology and Genetics, Faculty of Science, Istanbul University, 34134 Vezneciler-Istanbul, Turkey. earican@istanbul.edu.tr
authors.forename: Ercan
authors.initials: E
authors.lastname: Arican
authors.forename: Gulruh
authors.initials: G
authors.lastname: Albayrak
authors.forename: Nermin
authors.initials: N
authors.lastname: Gozukirmizi
mesh_terms: D028202:Abies / Q000201:enzymology* / Q000254:growth & development / Q000502:physiology; D018384:Oxidative Stress; D009195:Peroxidase / Q000378:metabolism*; D010940:Plant Proteins / Q000378:metabolism*; D018520:Plant Shoots / Q000201:enzymology / Q000254:growth & development / Q000502:physiology; D012038:Regeneration; D013482:Superoxide Dismutase / Q000378:metabolism*; D046509:Tissue Culture Techniques
chemical_list: D010940:Plant Proteins; D009195:Peroxidase; D013482:Superoxide Dismutase