Isolation and mapping of a C3'H gene (CYP98A49) from globe artichoke, and its expression upon UV-C stress.
Globe artichoke represents a natural source of phenolic compounds with dicaffeoylquinic acids along with their biosynthetic precursor chlorogenic acid (5-caffeoylquinic acid) as the predominant molecules. We report the isolation and characterization of a full-length cDNA and promoter of a globe artichoke p-coumaroyl ester 3'-hydroxylase (CYP98A49), which is involved in both chlorogenic acid and lignin biosynthesis. Phylogenetic analyses demonstrated that this gene belongs to the CYP98 family. CYP98A49 was also heterologously expressed in yeast, in order to perform an enzymatic assay with p-coumaroylshikimate and p-coumaroylquinate as substrates. Real Time quantitative PCR analysis revealed that CYP98A49 expression is induced upon exposure to UV-C radiation. A single nucleotide polymorphism in the CYP98A49 gene sequence of two globe artichoke varieties used for genetic mapping allowed the localization of this gene to linkage group 10 within the previously developed maps.
Moglia
Andrea
A
DiVaPRA, Plant Genetics and Breeding, University of Torino, via L. da Vinci 44, 10095, Grugliasco (TO), Italy.
Comino
Cinzia
C
Portis
Ezio
E
Acquadro
Alberto
A
De Vos
Ric C H
RC
Beekwilder
Jules
J
Lanteri
Sergio
S
D000595:Amino Acid Sequence; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D031943:Cynara scolymus / Q000235:genetics* / Q000528:radiation effects; D018744:DNA, Plant / Q000235:genetics; D018506:Gene Expression Regulation, Plant / Q000528:radiation effects*; D017343:Genes, Plant; D006899:Mixed Function Oxygenases / Q000235:genetics*; D008969:Molecular Sequence Data; D015394:Molecular Structure; D010940:Plant Proteins / Q000235:genetics; D020641:Polymorphism, Single Nucleotide; D011401:Promoter Regions, Genetic; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D014466:Ultraviolet Rays
D018744:DNA, Plant; D010940:Plant Proteins; D006899:Mixed Function Oxygenases