The transcriptional coactivator MAML1 regulates p300 autoacetylation and HAT activity.
MAML1 is a transcriptional coregulator originally identified as a Notch coactivator. MAML1 is also reported to interact with other coregulator proteins, such as CDK8 and p300, to modulate the activity of Notch. We, and others, previously showed that MAML1 recruits p300 to Notch-regulated genes through direct interactions with the DNA-CSL-Notch complex and p300. MAML1 interacts with the C/H3 domain of p300, and the p300-MAML1 complex specifically acetylates lysines of histone H3 and H4 tails in chromatin in vitro. In this report, we show that MAML1 potentiates p300 autoacetylation and p300 transcriptional activation. MAML1 directly enhances p300 HAT activity, and this coincides with the translocation of MAML1, p300 and acetylated histones to nuclear bodies.
Hansson
Magnus L
ML
Department of Biosciences and Nutrition, Karolinska Institutet, Stockholm, Sweden.
Popko-Scibor
Anita E
AE
Saint Just Ribeiro
Mariana
M
Dancy
Beverley M
BM
Lindberg
Mikael J
MJ
Cole
Philip A
PA
Wallberg
Annika E
AE
D000107:Acetylation; D002460:Cell Line; D006657:Histones / Q000378:metabolism; D017434:Protein Structure, Tertiary; D017384:Sequence Deletion; D015534:Trans-Activators / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D050880:p300-CBP Transcription Factors / Q000737:chemistry / Q000378:metabolism*; D000099224:p300-CBP-Associated Factor
D006657:Histones; D015534:Trans-Activators; D050880:p300-CBP Transcription Factors; D000099224:p300-CBP-Associated Factor