title: Genetic and phenotypic diversity of fenitrothion-degrading bacteria isolated from soils.
abstract: Twenty-seven fenitrothion-degrading bacteria were isolated from different soils, and their genetic and phenotypic characteristics were investigated. Analysis of the 16S rDNA sequence showed that the isolates were related to members of the genera Burkholderia, Pseudomonas, Sphingomonas, Cupriavidus, Corynebacterium, and Arthrobacter. Among the 27 isolates, 12 different chromosomal DNA fingerprinting patterns were obtained by polymerase chain reaction (PCR) amplification of repetitive extragenic palindromic (REP) sequences. The isolates were able to utilize fenitrothion as a sole source of carbon and energy, producing 3-methyl-4- nitrophenol as the intermediate metabolite during the complete degradation of fenitrothion. Twenty-two of 27 isolates were able to degrade parathion, methyl-parathion, and p-nitrophenol, but only strain BS2 could degrade EPN (O-ethyl-O-p-nitrophenyl phenylphosphorothioate) as a sole source of carbon and energy for growth. Eighteen of the 27 isolates had plasmids. When analyzed with PCR amplification and dot-blotting hybridization using various specific primers targeted to the organophosphorus pesticide hydrolase genes of the previously reported isolates, none of the isolates showed positive signals, suggesting that the corresponding genes of our isolates had no significant sequence homology with those of the previously isolated organophosphate pesticide-degrading bacteria.
authors.affiliation: Department of Agricultural Biotechnology, Seoul National University, Seoul 151-742, Korea.
authors.forename: Kyung-Duk
authors.initials: KD
authors.lastname: Kim
authors.forename: Jae-Hyung
authors.initials: JH
authors.lastname: Ahn
authors.forename: Taesung
authors.initials: T
authors.lastname: Kim
authors.forename: Seong Chan
authors.initials: SC
authors.lastname: Park
authors.forename: Chi Nam
authors.initials: CN
authors.lastname: Seong
authors.forename: Hong-Gyu
authors.initials: HG
authors.lastname: Song
authors.forename: Jong-Ok
authors.initials: JO
authors.lastname: Ka
mesh_terms: D001419:Bacteria / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D016172:DNA Fingerprinting; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D005278:Fenitrothion / Q000378:metabolism*; D014644:Genetic Variation; D005838:Genotype; D007306:Insecticides / Q000378:metabolism*; D055029:Inverted Repeat Sequences; D010641:Phenotype; D010957:Plasmids / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012988:Soil Microbiology
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D007306:Insecticides; D012336:RNA, Ribosomal, 16S; D005278:Fenitrothion