In situ molecular level studies on membrane related peptides and proteins in real time using sum frequency generation vibrational spectroscopy.
Sum frequency generation (SFG) vibrational spectroscopy has been demonstrated to be a powerful technique to study the molecular structures of surfaces and interfaces in different chemical environments. This review summarizes recent SFG studies on hybrid bilayer membranes and substrate-supported lipid monolayers and bilayers, the interaction between peptides/proteins and lipid monolayers/bilayers, and bilayer perturbation induced by peptides/proteins. To demonstrate the ability of SFG to determine the orientations of various secondary structures, studies on the interactions between different peptides/proteins (melittin, G proteins, alamethicin, and tachyplesin I) and lipid bilayers are discussed. Molecular level details revealed by SFG in these studies show that SFG can provide a unique understanding on the interactions between a lipid monolayer/bilayer and peptides/proteins in real time, in situ and without any exogenous labeling.
Ye
Shuji
S
Department of Chemistry, University of Michigan, Ann Arbor, 48109, USA.
Nguyen
Khoi Tan
KT
Le Clair
Stéphanie V
SV
Chen
Zhan
Z
D000818:Animals; D006801:Humans; D008051:Lipid Bilayers / Q000737:chemistry; D008565:Membrane Proteins / Q000737:chemistry; D010455:Peptides / Q000737:chemistry; D011506:Proteins / Q000737:chemistry*; D013057:Spectrum Analysis / Q000379:methods*; D014732:Vibration
D008051:Lipid Bilayers; D008565:Membrane Proteins; D010455:Peptides; D011506:Proteins