Interaction between Sox proteins of two physiologically distinct bacteria and a new protein involved in thiosulfate oxidation.
Organisms using the thiosulfate-oxidizing Sox enzyme system fall into two groups: group 1 forms sulfur globules as intermediates (Allochromatium vinosum), group 2 does not (Paracoccus pantotrophus). While several components of their Sox systems are quite similar, i.e. the proteins SoxXA, SoxYZ and SoxB, they differ by Sox(CD)(2) which is absent in sulfur globule-forming organisms. Still, the respective enzymes are partly exchangeable in vitro: P. pantotrophus Sox enzymes work productively with A. vinosum SoxYZ whereas A. vinosum SoxB does not cooperate with the P. pantotrophus enzymes. Furthermore, A. vinosum SoxL, a rhodanese-like protein encoded immediately downstream of soxXAK, appears to play an important role in recycling SoxYZ as it increases thiosulfate depletion velocity in vitro without increasing the electron yield.
Welte
Cornelia
C
Institut für Mikrobiologie & Biotechnologie, Rheinische Friedrich-Wilhelms-Universität Bonn, Meckenheimer Allee 168, D-53115 Bonn, Germany.
Hafner
Swetlana
S
Krätzer
Christian
C
Quentmeier
Armin
A
Friedrich
Cornelius G
CG
Dahl
Christiane
C
D002841:Chromatiaceae / Q000235:genetics / Q000378:metabolism*; D007700:Kinetics; D010084:Oxidation-Reduction; D042084:Paracoccus pantotrophus / Q000235:genetics / Q000378:metabolism*; D011485:Protein Binding; D055747:SOX Transcription Factors / Q000235:genetics / Q000378:metabolism*; D013885:Thiosulfates / Q000378:metabolism*
D055747:SOX Transcription Factors; D013885:Thiosulfates