Tryptophan 13C nuclear-spin polarization generated by intraprotein electron transfer in a LOV2 domain of the blue-light receptor phototropin.
(13)C-NMR experiments were performed on photo-excited fully and partially (13)C-labelled LOV2 domains of the blue-light receptor phototropin. In the present paper, we report on nuclear-spin polarized tryptophan resonances that are generated by light-induced intraprotein electron transfer to the FMN cofactor. The spectra are discussed with respect to earlier data obtained from (13)C-NMR experiments on unlabelled LOV2 domains that have been reconstituted with FMN (13)C isotopologues.
Eisenreich
Wolfgang
W
Department Chemie, Technische Universität München, Garching, Germany.
Fischer
Markus
M
Römisch-Margl
Werner
W
Joshi
Monika
M
Richter
Gerald
G
Bacher
Adelbert
A
Weber
Stefan
S
D002247:Carbon Isotopes; D056931:Cryptochromes; D004579:Electron Transport; D005486:Flavin Mononucleotide / Q000737:chemistry / Q000528:radiation effects; D005420:Flavoproteins / Q000737:chemistry* / Q000528:radiation effects; D008027:Light; D009682:Magnetic Resonance Spectroscopy; D017434:Protein Structure, Tertiary; D014364:Tryptophan / Q000737:chemistry*
D002247:Carbon Isotopes; D056931:Cryptochromes; D005420:Flavoproteins; D005486:Flavin Mononucleotide; D014364:Tryptophan