title: Distinct neutrophil subpopulations phenotype by flow cytometry in myelodysplastic syndromes.
abstract: The cardinal feature of myelodysplastic syndromes (MDS) is dysplasia involving one or more myeloid cell lineages. In the present study, we used 4-color flow cytometric analysis to investigate dysgranulopoiesis in bone marrow specimens from 65 patients with MDS. The antigen expression patterns of total neutrophil granulocytes (TNG) and of the two distinct neutrophil granulocytic subpopulations (NGSs), NGS-1 (dimmer CD45 expression) and NGS-2 (stronger CD45 expression) identified on the side scatter (SS) vs. CD45-intensity plot, were studied. The neutrophil granulocytes from patients with MDS showed characteristic antigen expression aberrancies which were more pronounced in NGS-2 subpopulation. Studying separately the NGS-2 subpopulation with the CD16/MPO/LF combination, the low CD16(+)/MPO(+) and low CD16(+)/LF(+) percentages seemed to discriminate between lower-risk and higher-risk patients with MDS in most occasions. Furthermore, a detailed assessment of the NGS-1 and NGS-2 immunophenotypic patterns revealed early dysplastic changes, not otherwise observed by standard TNG analysis, especially in cases of lower-risk MDS.
authors.affiliation: Department of Chemistry, National and Kapodistrian University of Athens, Athens, Greece. mvikentiou@biol.uoa.gr
authors.forename: Myrofora
authors.initials: M
authors.lastname: Vikentiou
authors.forename: Katerina
authors.initials: K
authors.lastname: Psarra
authors.forename: Violetta
authors.initials: V
authors.lastname: Kapsimali
authors.forename: Konstantinos
authors.initials: K
authors.lastname: Liapis
authors.forename: Michalis
authors.initials: M
authors.lastname: Michael
authors.forename: Konstantinos
authors.initials: K
authors.lastname: Tsionos
authors.forename: Evi
authors.initials: E
authors.lastname: Lianidou
authors.forename: Chryssa
authors.initials: C
authors.lastname: Papasteriades
mesh_terms: D000954:Antigens, Surface / Q000032:analysis; D001853:Bone Marrow / Q000473:pathology; D005434:Flow Cytometry / Q000379:methods*; D006801:Humans; D016130:Immunophenotyping; D017493:Leukocyte Common Antigens / Q000032:analysis; D009190:Myelodysplastic Syndromes / Q000175:diagnosis / Q000473:pathology*; D009504:Neutrophils / Q000473:pathology*; D017452:Receptors, IgG; D018570:Risk Assessment
chemical_list: D000954:Antigens, Surface; D017452:Receptors, IgG; D017493:Leukocyte Common Antigens