Inhibition of LPS-induced pulmonary inflammation by specific flavonoids.
In the present study, the anti-inflammatory effects of the flavonoids flavone, fisetin and tricetin were evaluated in a mouse model of LPS-induced acute pulmonary inflammation. The flavonoid fisetin significantly reduced lung myeloperoxidase-levels and gene-expression of inflammatory mediators such as IL-6, TNF-alpha, IL-1beta, MIP-1alpha and MIP-2. The LPS-induced gene transcription of HO-1 and SOD2 was also significantly reduced by fisetin. Overall, the anti-inflammatory effects of fisetin in this in vivo model were much more pronounced as compared to the observed effects of flavone or tricetin and the anti-inflammatory glucocorticoid dexamethasone. The results of this study indicate that flavonoids such as fisetin might be potential candidates as pharmaceuticals or nutraceuticals in the treatment of pulmonary inflammatory diseases.
Geraets
Liesbeth
L
Department of Pharmacology and Toxicology, Maastricht University Maastricht, The Netherlands. liesbeth.geraets@rivm.nl
Haegens
Astrid
A
Brauers
Karen
K
Haydock
Jane A
JA
Vernooy
Juanita H J
JH
Wouters
Emiel F M
EF
Bast
Aalt
A
Hageman
Geja J
GJ
D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000627:therapeutic use*; D002867:Chromones / Q000627:therapeutic use*; D047309:Flavones; D005419:Flavonoids / Q000627:therapeutic use*; D044948:Flavonols; D015870:Gene Expression; D051547:Heme Oxygenase-1 / Q000235:genetics; D018836:Inflammation Mediators / Q000037:antagonists & inhibitors / Q000378:metabolism; D015850:Interleukin-6 / Q000097:blood; D008070:Lipopolysaccharides / Q000276:immunology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D020556:Neutrophil Infiltration; D009195:Peroxidase / Q000037:antagonists & inhibitors; D011014:Pneumonia / Q000188:drug therapy* / Q000276:immunology; D000071137:Poly (ADP-Ribose) Polymerase-1; D011065:Poly(ADP-ribose) Polymerases / Q000378:metabolism; D013482:Superoxide Dismutase / Q000235:genetics; D000099156:Superoxide Dismutase 2
D000894:Anti-Inflammatory Agents, Non-Steroidal; D002867:Chromones; D047309:Flavones; D005419:Flavonoids; D044948:Flavonols; D051547:Heme Oxygenase-1; D018836:Inflammation Mediators; D015850:Interleukin-6; D008070:Lipopolysaccharides; D009195:Peroxidase; D000071137:Poly (ADP-Ribose) Polymerase-1; D011065:Poly(ADP-ribose) Polymerases; D013482:Superoxide Dismutase; D000099156:Superoxide Dismutase 2; C469689:tricetin; C499321:Parp1 protein, mouse; C017875:fisetin; C043562:flavone