Trade-off between immune stimulation and expression of storage protein genes.
Proteins stored in insect hemolymph may serve as a source of amino acids and energy for metabolism and development. The expression of the main storage proteins was assessed in bacterial-challenged honey bees using real-time (RT)-PCR and Western blot. After ensuring that the immune system had been activated by measuring the ensuing expression of the innate immune response genes, defensin-1 (def-1) and prophenoloxidase (proPO), we verified the expression of four genes encoding storage proteins. The levels of vitellogenin (vg) mRNA and of the respective protein were significantly lowered in bees injected with bacteria or water only (injury). An equivalent response was observed in orally-infected bees. The levels of apolipophorin II/I (apoLp-II/I) and hexamerin (hex 70a) mRNAs did not significantly change, but levels of Hex 70a protein subunit showed a substantial decay after bacterial challenge or injury. Infection also caused a strong reduction in the levels of apoLp-III transcripts. Our findings are consistent with a down-regulation of the expression and accumulation of storage proteins as a consequence of activation of the immune system, suggesting that this phenomenon represents a strategy to redirect resources to combat injury or infection.
Lourenço
Anete P
AP
Departamento de Genética, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, São Paulo, Brazil. anetelourenco@gmail.com
Martins
Juliana R
JR
Bitondi
Márcia M G
MM
Simões
Zilá L P
ZL
D000818:Animals; D001053:Apolipoproteins / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D001424:Bacterial Infections / Q000276:immunology* / Q000378:metabolism; D001516:Bees / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D004156:Catechol Oxidase / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D023082:Defensins / Q000276:immunology / Q000378:metabolism; D004792:Enzyme Precursors / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D005260:Female; D005786:Gene Expression Regulation; D006458:Hemolymph / Q000276:immunology* / Q000378:metabolism; D019476:Insect Proteins / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D012313:RNA / Q000032:analysis; D012333:RNA, Messenger / Q000032:analysis; D013045:Species Specificity; D013312:Stress, Physiological / Q000235:genetics / Q000276:immunology; D014819:Vitellogenins / Q000235:genetics / Q000276:immunology / Q000378:metabolism
D001053:Apolipoproteins; D023082:Defensins; D004792:Enzyme Precursors; D019476:Insect Proteins; D012333:RNA, Messenger; D014819:Vitellogenins; C042470:apolipophorin III; C041137:storage proteins, Insecta; D012313:RNA; C038451:pro-phenoloxidase; D004156:Catechol Oxidase