title: Soluble HLA-G in IVF/ICSI embryo culture supernatants does not always predict implantation success: a multicentre study.
abstract: Several reports have described an association between the presence of soluble human leukocyte antigen G (sHLA-G) in human embryo culture supernatants (ES) and implantation success. However, not all studies agree with these findings. To further document this debate, a multicentre blinded study was performed to investigate, on a large number of IVF ES and ICSI ES, whether sHLA-G is a useful criterion for embryo selection before transfer. A total of 1405 ES from 355 patients were collected from three assisted reproductive technique (ART) centres and evaluated for their sHLA-G content in a single laboratory, using a chemiluminescence enzyme-linked immunosorbent assay. In only one centre was a significant association between sHLA-G-positive ES and successful implantation established (P = 0.0379), whereas no such association was observed in the other centres. It was found that the percentages and concentrations of sHLA-G-positive ES varied between centres, depending on culture media and ART conditions. The percentage of sHLA-G-positive ES was significantly higher in IVF ES than ICSI ES (P < 0.001 and P < 0.01 for two centres). These data demonstrate that substantial variations of sHLA-G content in ES occur between different ART centres, highlighting the influence of several technical parameters that differ from one centre to another.
authors.affiliation: INSERM, U563, Centre de Physiopathologie de Toulouse Purpan, Toulouse, F-31300 France.
authors.forename: Julie
authors.initials: J
authors.lastname: Tabiasco
authors.forename: Sophie
authors.initials: S
authors.lastname: Perrier d'Hauterive
authors.forename: Fabienne
authors.initials: F
authors.lastname: Thonon
authors.forename: Jean
authors.initials: J
authors.lastname: Parinaud
authors.forename: Roger
authors.initials: R
authors.lastname: Léandri
authors.forename: Jean-Michel
authors.initials: JM
authors.lastname: Foidart
authors.forename: Gérard
authors.initials: G
authors.lastname: Chaouat
authors.forename: Carine
authors.initials: C
authors.lastname: Munaut
authors.forename: Raoul
authors.initials: R
authors.lastname: Lombroso
authors.forename: Jacqueline
authors.initials: J
authors.lastname: Selva
authors.forename: Marianne
authors.initials: M
authors.lastname: Bergère
authors.forename: Ibrahim
authors.initials: I
authors.lastname: Hammoud
authors.forename: Noemi
authors.initials: N
authors.lastname: Kozma
authors.forename: Maryse
authors.initials: M
authors.lastname: Aguerre-Girr
authors.forename: Anna K E
authors.initials: AK
authors.lastname: Swales
authors.forename: Ian L
authors.initials: IL
authors.lastname: Sargent
authors.forename: Philippe
authors.initials: P
authors.lastname: Le Bouteiller
authors.forename: Nathalie
authors.initials: N
authors.lastname: Lédée
mesh_terms: D000328:Adult; D003470:Culture Media; D004797:Enzyme-Linked Immunosorbent Assay; D005307:Fertilization in Vitro; D006680:HLA Antigens / Q000032:analysis*; D059951:HLA-G Antigens; D015395:Histocompatibility Antigens Class I / Q000032:analysis*; D006801:Humans; D049449:Luminescence; D020554:Sperm Injections, Intracytoplasmic
chemical_list: D003470:Culture Media; D006680:HLA Antigens; D059951:HLA-G Antigens; D015395:Histocompatibility Antigens Class I