Generation of transgenic mice expressing Cre recombinase under the control of the Dll1 mesoderm enhancer element.
To study paraxial mesoderm formation in the mouse, transgenic lines that can be used to either selectively delete or express genes of interest in the paraxial mesoderm are required. We have generated a transgenic mouse line that expresses Cre recombinase in the paraxial mesoderm (PAM) beginning at e7.5. A lacZ Cre recombinase reporter line showed that in addition to PAM and its derivatives, lateral plate and intermediate mesoderm derivatives were also exposed to Cre activity, while the node, notochord, and cardiac mesoderm were not. We further demonstrate that 70-75% of the fibroblasts generated from Dll1-msd Cre, ROSA26-rtTA embryos possess Cre recombinase activity. These mice can therefore be used in combination with tet-responsive transgenic lines to generate mesoderm-derived embryonic fibroblasts that inducibly express a gene of interest.
Wehn
Amy K
AK
Department of Biological Sciences, University of Pittsburgh, Pennsylvania, USA.
Gallo
Phillip H
PH
Chapman
Deborah L
DL
D000818:Animals; D002135:Calcium-Binding Proteins; D004622:Embryo, Mammalian / Q000196:embryology / Q000378:metabolism; D004742:Enhancer Elements, Genetic / Q000235:genetics*; D005347:Fibroblasts / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D019426:Integrases / Q000235:genetics* / Q000378:metabolism; D036341:Intercellular Signaling Peptides and Proteins / Q000235:genetics*; D008648:Mesoderm / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D018613:Microscopy, Confocal; D011506:Proteins / Q000235:genetics / Q000378:metabolism; D022661:RNA, Untranslated; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism
D002135:Calcium-Binding Proteins; C512925:Dlk1 protein, mouse; C110853:Gt(ROSA)26Sor non-coding RNA, mouse; D036341:Intercellular Signaling Peptides and Proteins; D011506:Proteins; D022661:RNA, Untranslated; D011993:Recombinant Fusion Proteins; D049452:Green Fluorescent Proteins; C045073:Cre recombinase; D019426:Integrases