Label-free detection of B. anthracis spores using a surface plasmon resonance biosensor.
This study demonstrates the first use of surface plasmon resonance (SPR) technology for the rapid, sensitive and label-free detection of whole B. anthracis spores. The approach involves the use of an SPR biosensor (Biacore 3000), and a monoclonal antibody which was raised against the B. anthracis spore (mAb 8G3). By means of subtractive inhibition assays, whole B. anthracis spores with concentrations as low as 10(4) colony-forming units (CFU) ml(-1) can be detected within 40 min, and other related Bacillus spores, even in high concentrations, can be differentiated from B. anthracis spores.
Wang
Dian-Bing
DB
State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, 430071, China.
Bi
Li-Jun
LJ
Zhang
Zhi-Ping
ZP
Chen
Yuan-Yuan
YY
Yang
Rui-Fu
RF
Wei
Hong-Ping
HP
Zhou
Ya-Feng
YF
Zhang
Xian-En
XE
D000911:Antibodies, Monoclonal / Q000737:chemistry; D000942:Antigens, Bacterial / Q000737:chemistry; D001408:Bacillus anthracis / Q000502:physiology*; D013171:Spores, Bacterial; D020349:Surface Plasmon Resonance / Q000379:methods*
D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial