title: Siglec-1 on monocytes is a potential risk marker for monitoring disease severity in coronary artery disease.
abstract: OBJECTIVE
  Siglec-1 has long been considered as an important biomarker of the activation of monocyte/macrophage and a type I interferon-specific imprint, but its role in atherosclerosis has not been elucidated.
  
  
  METHODS
  We examined the expression of Siglec-1 by flow cytometry and RT-PCR in 83 CAD patients and 38 healthy controls. In addition, the levels of serum lipids, Gensini score, hs-CRP and homocysteine were determined.
  
  
  RESULTS
  The transcriptional and protein levels of Siglec-1 on monocytes in CAD patients were significantly increased compared with healthy controls [3.17 versus 1.0, P<0.01; (11.5+/-3.9)% versus (1.8+/-2.0)%, P<0.01], but the increased Siglec-1 had no correlation with the level of native serum lipids. Interestingly, the expression of Siglec-1 was positively correlated with Gensini score (r=0.338, P=0.015), hs-CRP (r=0.316, P=0.016) and homocysteine level (r=0.224, P=0.042).
  
  
  CONCLUSIONS
  Siglec-1 may be considered as a potential non-invasive indicator for monitoring disease severity and a biomarker for predicting the relative risk of cardiovascular events.
authors.affiliation: Department of Laboratory Medicine, Changzheng Hospital, Second Military Medical University and Clinical Immunology Center of PLA, 415 Feng Yang Road, Huang Pu District, Shanghai 200003, People's Republic of China.
authors.forename: Yi-song
authors.initials: YS
authors.lastname: Xiong
authors.forename: Yun-heng
authors.initials: YH
authors.lastname: Zhou
authors.forename: Guang-hua
authors.initials: GH
authors.lastname: Rong
authors.forename: Wei-lin
authors.initials: WL
authors.lastname: Wu
authors.forename: Yan
authors.initials: Y
authors.lastname: Liang
authors.forename: Zai-xing
authors.initials: ZX
authors.lastname: Yang
authors.forename: Hong-lian
authors.initials: HL
authors.lastname: Geng
authors.forename: Ren-qian
authors.initials: RQ
authors.lastname: Zhong
mesh_terms: D015415:Biomarkers / Q000378:metabolism; D002097:C-Reactive Protein / Q000032:analysis; D003324:Coronary Artery Disease / Q000097:blood / Q000235:genetics / Q000378:metabolism* / Q000473:pathology*; D004587:Electrophoresis, Agar Gel; D005434:Flow Cytometry; D005786:Gene Expression Regulation; D006710:Homocysteine / Q000097:blood; D006801:Humans; D008562:Membrane Glycoproteins / Q000378:metabolism*; D009000:Monocytes / Q000378:metabolism*; D012313:RNA / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011971:Receptors, Immunologic / Q000378:metabolism*; D012307:Risk Factors; D063267:Sialic Acid Binding Ig-like Lectin 1
chemical_list: D015415:Biomarkers; D008562:Membrane Glycoproteins; D012333:RNA, Messenger; D011971:Receptors, Immunologic; C571166:SIGLEC1 protein, human; D063267:Sialic Acid Binding Ig-like Lectin 1; D006710:Homocysteine; D012313:RNA; D002097:C-Reactive Protein