title: Bone regeneration using beta-tricalcium phosphate in a calcium sulfate matrix.
abstract: The aim of the study was the histomorphometric comparison of the osteogenic potential of beta-tricalcium phosphate (beta-TCP) alone or in a calcium sulfate matrix. Three round defects, 10 mm (diameter) x 5 mm (depth), were created on each iliac crest of 4 dogs. The defects were divided into 3 groups. Ten defects were filled with beta-TCP in a calcium sulfate (CS) matrix (Fortoss Vital; group A), 10 defects were filled with beta-TCP alone (Fortoss Resorb; group B), and 4 defects were left ungrafted to heal spontaneously (group C). All defects were left to heal for 4 months without the use of a barrier membrane. Histologic evaluation and morphometric analysis of undecalcified slides was performed using the areas of regenerated bone and graft remnants. All sites exhibited uneventful healing. In group A sites (beta-TCP/CS), complete bone formation was observed in all specimens, graft granules dominated the area, and a thin bridge of cortical bone was covering the defect. Group B (beta-TCP) defects were partially filled with new bone, the graft particles still dominated the area, while the outer cortex was not restored. In the ungrafted sites (group C), incomplete new bone formation was observed. The outer dense cortical layer was restored in a lower level, near the base of the defect. The statistical analysis revealed that the mean percentage of new bone regeneration in group A was higher than in group B (49.38% and 40.31%, respectively). A statistically significant difference existed between the 2 groups. The beta-TCP/CS group exhibited significantly higher new bone regeneration according to a marginal probability value (P = .004 < .05). The use of beta-TCP in a CS matrix produced significantly more vital new bone fill and preserved bone dimensions compared with the use of beta-TCP alone.
authors.affiliation: Department of Oral and Maxillofacial Surgery, Dental School, University of Athens (EKPA), Greece. lpodar@otenet.gr
authors.forename: Leonidas
authors.initials: L
authors.lastname: Podaropoulos
authors.forename: Alexander A
authors.initials: AA
authors.lastname: Veis
authors.forename: Serafim
authors.initials: S
authors.lastname: Papadimitriou
authors.forename: Constantinos
authors.initials: C
authors.lastname: Alexandridis
authors.forename: Demos
authors.initials: D
authors.lastname: Kalyvas
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000627:therapeutic use*; D001861:Bone Regeneration / Q000502:physiology*; D018786:Bone Substitutes / Q000627:therapeutic use*; D002130:Calcium Phosphates / Q000627:therapeutic use*; D002133:Calcium Sulfate / Q000737:chemistry*; D003238:Connective Tissue / Q000473:pathology; D004285:Dogs; D004337:Drug Carriers; D004848:Epithelium / Q000473:pathology; D006253:Haversian System / Q000473:pathology; D007085:Ilium / Q000473:pathology / Q000601:surgery; D007091:Image Processing, Computer-Assisted; D023421:Models, Animal; D010012:Osteogenesis / Q000502:physiology; D014741:Video Recording; D014945:Wound Healing / Q000502:physiology
chemical_list: D001672:Biocompatible Materials; D018786:Bone Substitutes; D002130:Calcium Phosphates; D004337:Drug Carriers; C485817:beta-tricalcium phosphate; D002133:Calcium Sulfate