title: [Mass chromatographic analysis on different protein expression of human periodontal ligament cell under static pressure].
abstract: OBJECTIVE
  To analyze the protein expression of human periodontal ligament cells (HPDLCs) under static pressure.
  
  
  METHODS
  The proteins of two groups of human periodontal ligament cell (one group was interfered by static pressure and one group was not) were analyzed by two-dimensional electrophoresis and mass chromatographic analysis. The silver stained proteins spots were analyzed by Image Master 2D Platinum Software 5.0. There were about 720 and 730 detectable spots on the two 2D-gels separately and nearly 30 spots which were differentially expressed.
  
  
  RESULTS
  With direct MALDI-TOF mass spectrometry and protein database searching, 2 protein spots out of 5 new appeared spots were identified. Presenilin 2 and catechol O-methyltransferase were expressed only in the group which was interfered by static pressure.
  
  
  CONCLUSIONS
  The results of mass chromatographic analysis demonstrate that Notch pathway and calcitonin gene-related peptide(CGRP) may be the partners of the channel of biomechanical signal conduction of the periodontal ligament cell.
authors.affiliation: Department of Orthodontics, Medical School of Xi'an Jiaotong University, Xi'an 710004, China. ann@stu.xjtu.edu.cn
authors.forename: Yuan-Yuan
authors.initials: YY
authors.lastname: An
authors.forename: Hong
authors.initials: H
authors.lastname: Zhou
authors.forename: Yu-Song
authors.initials: YS
authors.lastname: Ruan
authors.forename: Guo-Zhou
authors.initials: GZ
authors.lastname: Rao
mesh_terms: D015180:Electrophoresis, Gel, Two-Dimensional; D006801:Humans; D010513:Periodontal Ligament; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization