title: Addition of zinc and manganese to a biological dressing.
abstract: BACKGROUND
  Zinc and manganese, which are used in vivo because of their healing properties, have been shown to modulate in vitro integrin expression and to enhance keratinocyte migration. In addition, at the clinical level, a dressing of keratinocytes suspended in a fibrin glue has been proposed for the treatment of chronic wounds.
  
  
  OBJECTIVE
  To investigate whether the addition of trace elements to this dressing could modulate the migratory phenotype of keratinocytes via the modulation of integrin expression in a manner similar to an in vitro model and thus increase the healing properties of this dressing.
  
  
  METHODS
  Keratinocytes were mixed with Tissucol and maintained in culture for 12 days in a medium either supplemented or not with zinc or manganese. Then, integrin expression was studied by immunohistochemistry on fibrin clot cryosections.
  
  
  RESULTS
  We observed a significant increase of alpha5beta1 with zinc compared to the control medium. Zinc also enhanced alphaVbeta6 expression and manganese alpha5beta1, alphaVbeta5 and alphaVbeta6 expression, however without reaching a significant level.
  
  
  CONCLUSIONS
  By modulating integrin expression, trace elements can improve the efficiency of a biological dressing made of keratinocytes in a fibrin glue matrix and, thus, it appears beneficial to add them to this biological dressing for the treatment of skin defects.
authors.affiliation: UTCG, CHU Nantes, Institut de Biologie, Nantes Cedex, France.
authors.forename: I
authors.initials: I
authors.lastname: Tenaud
authors.forename: I
authors.initials: I
authors.lastname: Saiagh
authors.forename: B
authors.initials: B
authors.lastname: Dreno
mesh_terms: D001684:Biological Dressings; D002465:Cell Movement / Q000187:drug effects / Q000502:physiology; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D006801:Humans; D007150:Immunohistochemistry; D016023:Integrins / Q000378:metabolism*; D015603:Keratinocytes / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D016014:Linear Models; D008345:Manganese / Q000378:metabolism / Q000494:pharmacology*; D011336:Probability; D012016:Reference Values; D012680:Sensitivity and Specificity; D012867:Skin / Q000187:drug effects / Q000378:metabolism; D014131:Trace Elements / Q000378:metabolism / Q000494:pharmacology; D014945:Wound Healing / Q000187:drug effects / Q000502:physiology*; D015032:Zinc / Q000378:metabolism / Q000494:pharmacology*
chemical_list: D016023:Integrins; D014131:Trace Elements; D008345:Manganese; D015032:Zinc