title: Study of the depletion of lincomycin residues in honey extracted from treated honeybee (Apis mellifera L.) colonies and the effect of the shook swarm procedure.
abstract: Bee colonies were treated with 1.2g lincomycin hydrochloride per hive (single treatment in sucrose solution) and samples of honey were then collected at intervals over a 41-week period. The samples were analysed for lincomycin using Liquid Chromatography-Mass Spectrometry/Mass Spectrometry (LC-MS/MS). The highest mean concentration of lincomycin (pooled analytical results for brood and super honey) was 24 microugg(-1) 3 days after treatment, a mean of 3.5 microgg(-1) after 129 days. The shook swarm procedure was investigated and resulted in a lincomycin concentration of 34 microgg(-1) in honey (pooled results for brood and super honey) 3 days after treatment, declining to 0.38 microgg(-1) 129 days after treatment. Lincomycin was persistent in the hive and detected in all over winter (290 days after dosing) samples of honey collected from both non-shook swarmed and shook swarmed colonies. The results overall indicate that lincomycin parent is a suitable marker compound to detect lincomycin misuse in apiculture.
authors.affiliation: Food Science Group, Central Science Laboratory, Sand Hutton, York YO41 1LZ, United Kingdom.
authors.forename: Stuart J
authors.initials: SJ
authors.lastname: Adams
authors.forename: Richard J
authors.initials: RJ
authors.lastname: Fussell
authors.forename: Mike
authors.initials: M
authors.lastname: Dickinson
authors.forename: Selwyn
authors.initials: S
authors.lastname: Wilkins
authors.forename: Matthew
authors.initials: M
authors.lastname: Sharman
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000008:administration & dosage / Q000032:analysis*; D001516:Bees / Q000737:chemistry* / Q000187:drug effects; D002851:Chromatography, High Pressure Liquid; D004350:Drug Residues / Q000032:analysis*; D005506:Food Contamination / Q000032:analysis*; D006722:Honey / Q000032:analysis*; D008034:Lincomycin / Q000008:administration & dosage / Q000032:analysis*; D053719:Tandem Mass Spectrometry; D013997:Time Factors
chemical_list: D000900:Anti-Bacterial Agents; D008034:Lincomycin