2-DE and LC-MS/MS for a comparative proteomic analysis of BALf from subjects with different subsets of inflammatory myopathies.
The protein profiles of bronchoalveolar lavage fluid (BALf) of patients belonging to three selected subsets of Polymyositis/Dermatomyositis (PM/DM) have been compared by using a combination of 2-DE and MALDI-TOF/MS or LC-MS/MS. Our study examined the hypothesis that there were distinct differences in protein expression profiles that were related to the phenotype. From among the 323+/-51 protein spots that may represent the most highly expressed proteins in BALf of these patients, 24 unique spots were isolated and proteins identified. In particular, 9 spots were present in BALf of PM/DM patients only; 12 spots were exclusive of Overlap patients and 3 spots of AS patients. From among the proteins identified, a few were classified as cytoskeletal proteins, others were involved in oxidative stress and a number of proteins were associated with general metabolic activity or immunological response and inflammation. This is the first study in which evidence is provided that a number of different proteins are expressed in different subsets of PM/DM and supports our contention that the proteomic approach would be beneficial in discovering molecules which could represent possible prognostic factors of these rare pathologies.
Passadore
Ileana
I
Department of Biochemistry A. Castellani, University of Pavia, Via Taramelli 3/B, 27100 Pavia, Italy.
Iadarola
Paolo
P
Di Poto
Cristina
C
Giuliano
Serena
S
Montecucco
Carlomaurizio
C
Cavagna
Lorenzo
L
Bonino
Claudia
C
Meloni
Federica
F
Fietta
Anna Maria
AM
Lisa
Antonella
A
Salvini
Roberta
R
Bardoni
Anna Maria
AM
D000368:Aged; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry*; D002853:Chromatography, Liquid; D051338:Cofilin 1 / Q000097:blood; D003882:Dermatomyositis / Q000097:blood / Q000378:metabolism* / Q000473:pathology; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods*; D018260:Gelsolin / Q000097:blood; D006801:Humans; D015151:Immunoblotting; D008875:Middle Aged; D011506:Proteins / Q000032:analysis / Q000145:classification / Q000378:metabolism; D040901:Proteomics / Q000379:methods*; D015203:Reproducibility of Results; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D053719:Tandem Mass Spectrometry; D014746:Vimentin / Q000097:blood
D051338:Cofilin 1; D018260:Gelsolin; D011506:Proteins; D014746:Vimentin