Optically modulated fluorophores for selective fluorescence signal recovery.
Fluorescence imaging in biological sciences is hindered by significant depth-dependent signal attenuation and highly fluorescent backgrounds. We have developed optically modulated near-IR-emitting few-atom Ag nanodots that are selectively and dynamically photobrightened upon simultaneous excitation with a secondary laser, enabling high-sensitivity image extraction to reveal only the demodulated fluorophores. Image demodulation is demonstrated in high-background environments to extract weak signals from completely obscuring background emission.
Richards
Chris I
CI
School of Chemistry and Biochemistry and Petit Institute for Biosciences and Bioengineering, Georgia Institute of Technology, Atlanta, Georgia 30332-0400, USA.
Hsiang
Jung-Cheng
JC
Senapati
Dulal
D
Patel
Sandeep
S
Yu
Junhua
J
Vosch
Tom
T
Dickson
Robert M
RM
D000818:Animals; D002232:Carbocyanines / Q000737:chemistry*; D003584:Cytological Techniques; D003952:Diagnostic Imaging; D005453:Fluorescence; D006046:Gold / Q000737:chemistry*; D051379:Mice; D041681:NIH 3T3 Cells; D043942:Nanotubes / Q000737:chemistry*; D055095:Optics and Photonics; D010777:Photochemistry
D002232:Carbocyanines; C085321:cyanine dye 5; D006046:Gold