[Research on the role of metabolism of fatty substance and osteoclast activity during the development of steroid-induced necrosis of femoral head].
OBJECTIVE
To explore the role of metabolism of fatty substance and osteoclast activity during the process of steroid-induced necrosis of femoral head through mice model inducing and model index measurement.


METHODS
Forty SD male mice were divided into 2 groups randomly, the control group and the experiment group. After the gluteal injection of colibacillus endotoxin,the experiment group was given gluteal injection of prednisolone acetate 35.5 mg/kg per week, and 2 ml of normal saline to the control group. The mice were killed 12 weeks later and tested the content of Trap-5b, TC and TG of the blood serum. Vitodynamics, bone density were measured and sections of HE staining, Ca2+ and TRACP staining was made then statistic analysis was performanced.


RESULTS
The content of TC, TG and Trap-5b increased apparently (P < 0.01). Large amount of osteoclasts were found in local medullary cavity. There was severe bone loss and decrease of vitodynamics in subchondral bone (P < 0.01) in experiment group.


CONCLUSIONS
Metabolic disorder of fatty substance is the key pathogenesis of steroid-induced avascular necrosis of femoral head. Decrease of vitodynamics in subchondral bone due to hyperactivity and increase of osteoclast lead to collapse of femoral head directly.
Tong
Pei-jian
PJ
Department of Orthopaedic, the First Affiliated Hospital of Zhejiang University of Traditional Chinese Medicine, Hangzhou 310006, Zhejiang, China.
Xiao
Lu-wei
LW
Ji
Wei-feng
WF
Tian
Kun
K
D000818:Animals; D015519:Bone Density; D004195:Disease Models, Animal; D005227:Fatty Acids / Q000378:metabolism*; D005271:Femur Head Necrosis / Q000378:metabolism* / Q000503:physiopathology; D006801:Humans; D008297:Male; D010010:Osteoclasts / Q000187:drug effects* / Q000378:metabolism*; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D013256:Steroids / Q000494:pharmacology*
D005227:Fatty Acids; D013256:Steroids