Conjunctival epithelial cells with donor-derived genome after human haematopoietic stem-cell transplantation.
To detect conjunctival epithelial cells with donor derived genome following human haematopoietic stem cell transplantation. Conjunctival biopsies from allotransplanted females were stained for the Y-chromosome, cytokeratin, and CD45 followed by a DAPI counterstain and CD68 staining and were then evaluated by laser-scanning confocal microscopy and 3D analysis. In 5 patients donor derived epithelial cells identified as Y+/DAPI+/CK+/CD45-/CD68-/epithelial morphology could be found (57%) with a mean incidence of 5.4% (range 2.42% - 7.94%). Our study demonstrates that besides the already published results in liver, colon, lung, skin and oral mucosa, epithelial cells with donor-derived genome also emerge in the conjunctiva following human HSCT. The biological significance and the underlying mechanism of these findings need further clarification.
Eberwein
Philipp
P
University Eye Hospital Freiburg, Freiburg, Germany. philipp.eberwein@uniklinik-freiburg.de
Faber
Philipp
P
Reinhard
Thomas
T
Finke
Juergen
J
Spyridonidis
Alexandros
A
D000328:Adult; D000368:Aged; D015703:Antigens, CD / Q000235:genetics; D001706:Biopsy; D041322:Chromosomes, Human, Y; D003228:Conjunctiva / Q000502:physiology*; D004847:Epithelial Cells / Q000502:physiology*; D016678:Genome; D006086:Graft vs Host Disease / Q000235:genetics; D018380:Hematopoietic Stem Cell Transplantation; D006801:Humans; D007166:Immunosuppressive Agents / Q000627:therapeutic use; D007938:Leukemia / Q000601:surgery; D017493:Leukocyte Common Antigens / Q000235:genetics; D008875:Middle Aged; D009092:Mucous Membrane / Q000502:physiology; D014019:Tissue Donors
D015703:Antigens, CD; D007166:Immunosuppressive Agents; D017493:Leukocyte Common Antigens