title: Friends and foes in synaptic transmission: the role of tomosyn in vesicle priming.
abstract: Priming is the process by which vesicles become available for fusion at nerve terminals and is modulated by numerous proteins and second messengers. One of the prominent members of this diverse family is tomosyn. Tomosyn has been identified as a syntaxin-binding protein; it inhibits vesicle priming, but its mode of action is not fully understood. The inhibitory activity of tomosyn depends on its N-terminal WD40-repeat domain and is regulated by the binding of its SNARE motif to syntaxin. Here, we describe new physiological information on the function of tomosyn and address possible interpretations of these results in the framework of the recently described crystal structure of the yeast tomosyn homolog Sro7. We also present possible molecular scenarios for vesicle priming and the involvement of tomosyn in these processes.
authors.affiliation: Department of Neurobiology, Faculty of Life Sciences, Tel Aviv University, Tel Aviv 69978, Israel. uria@post.tau.ac.il
authors.forename: Uri
authors.initials: U
authors.lastname: Ashery
authors.forename: Noa
authors.initials: N
authors.lastname: Bielopolski
authors.forename: Boaz
authors.initials: B
authors.lastname: Barak
authors.forename: Ofer
authors.initials: O
authors.lastname: Yizhar
mesh_terms: D000818:Animals; D006801:Humans; D013569:Synapses / Q000378:metabolism* / Q000648:ultrastructure; D009435:Synaptic Transmission / Q000502:physiology*; D013572:Synaptic Vesicles / Q000378:metabolism*; D033921:Vesicular Transport Proteins / Q000378:metabolism*
chemical_list: D033921:Vesicular Transport Proteins