title: Photosynthesis in cells around veins of the C(3) plant Arabidopsis thaliana is important for both the shikimate pathway and leaf senescence as well as contributing to plant fitness.
abstract: Cells associated with veins of C(3) species often contain significant amounts of chlorophyll, and radiotracer analysis shows that carbon present in the transpiration stream may be used for photosynthesis in these cells. It is not clear whether CO2 is also supplied to these cells close to veins via stomata, nor whether this veinal photosynthesis supplies carbon skeletons to particular metabolic pathways. In addition, it has not been possible to determine whether photosynthesis in cells close to veins of C(3) plants is quantitatively important for growth or fitness. To investigate the role of photosynthesis in cells in and around the veins of C(3) plants, we have trans-activated a hairpin construct to the chlorophyll synthase gene (CS) using an Arabidopsis thaliana enhancer trap line specific to veins. CS is responsible for addition of the phytol chain to the tetrapyrolle head group of chlorophyll, and, as a result of cell-specific trans-activation of the hairpin to CS, chlorophyll accumulation is reduced around veins. We use these plants to show that, under steady-state conditions, the extent to which CO2 is supplied to cells close to veins via stomata is limited. Fixation by minor veins of CO2 supplied to the xylem stream and the amount of specific metabolites associated with carbohydrate metabolism and the shikimate pathway were all reduced. In addition, an abundance of transcripts encoding components of pathways that generate phosphoenolpyruvate were altered. Leaf senescence, growth rate and seed size were all reduced in the lines with lower photosynthetic ability in veins and in cells close to veins.
authors.affiliation: Department of Plant Sciences, Downing Street, University of Cambridge, Cambridge CB23EA, UK.
authors.forename: Sophie H
authors.initials: SH
authors.lastname: Janacek
authors.forename: Sandra
authors.initials: S
authors.lastname: Trenkamp
authors.forename: Ben
authors.initials: B
authors.lastname: Palmer
authors.forename: Naomi J
authors.initials: NJ
authors.lastname: Brown
authors.forename: Kate
authors.initials: K
authors.lastname: Parsley
authors.forename: Susan
authors.initials: S
authors.lastname: Stanley
authors.forename: Holly M
authors.initials: HM
authors.lastname: Astley
authors.forename: Stephen A
authors.initials: SA
authors.lastname: Rolfe
authors.forename: W
authors.initials: W
authors.lastname: Paul Quick
authors.forename: Alisdair R
authors.initials: AR
authors.lastname: Fernie
authors.forename: Julian M
authors.initials: JM
authors.lastname: Hibberd
mesh_terms: D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D050260:Carbohydrate Metabolism; D002245:Carbon Dioxide / Q000378:metabolism; D019729:Carbon-Oxygen Ligases / Q000235:genetics / Q000378:metabolism; D002734:Chlorophyll / Q000096:biosynthesis*; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D010788:Photosynthesis; D030821:Plants, Genetically Modified / Q000235:genetics / Q000378:metabolism / Q000502:physiology; D034622:RNA Interference; D018749:RNA, Plant / Q000378:metabolism; D012765:Shikimic Acid / Q000378:metabolism*
chemical_list: D018749:RNA, Plant; D002734:Chlorophyll; D002245:Carbon Dioxide; D012765:Shikimic Acid; D019729:Carbon-Oxygen Ligases; C026901:chlorophyll synthetase