Confinement spectroscopy: probing single DNA molecules with tapered nanochannels.
We demonstrate a confinement spectroscopy technique capable of probing small conformational changes of unanchored single DNA molecules in a manner analogous to force spectroscopy, in the regime corresponding to femtonewton forces. In contrast to force spectroscopy, various structural forms of DNA can easily be probed, as indicated by experiments on linear and circular DNA. The extension of circular DNA is found to scale according to the de Gennes exponent, unlike for linear DNA.
Persson
Fredrik
F
Department of Micro- and Nanotechnology, Technical University of Denmark, DTU Nanotech, Kongens Lyngby, Denmark.
Utko
Pawel
P
Reisner
Walter
W
Larsen
Niels B
NB
Kristensen
Anders
A
D004247:DNA / Q000737:chemistry*; D015342:DNA Probes; D036103:Nanotechnology; D013057:Spectrum Analysis / Q000379:methods*
D015342:DNA Probes; D004247:DNA