The role of Id-1 in chemosensitivity and epirubicin-induced apoptosis in bladder cancer cells.
Recurrence and progression are the major problems in the treatment of bladder cancer. Increased expression of Id-1, a basic helix-loop-helix transcription factor, has recently been shown in several types of advanced cancer. Some studies have provided evidence to suggest that Id-1 can be considered a potential therapeutic target. The objective of this study was to investigate the role of Id-1 in the chemosensitivity of bladder cancer cells, and the effect of Id-1 on chemotherapeutic drug-induced apoptosis in bladder cancer cells. We compared the different sensitivity to epirubicin in RT112 and MGH-U1 cell lines with different Id-1 expression. Then, we transfected different vectors into RT112 and MGH-U1 respectively, and generated the stable Id-1 up-regulation and down-regulation transfectants. The results of cell viability assay showed up-regulation of Id-1 in RT112 leading to increased sensitivity in response to epirubicin, and down-regulation of Id-1 increased cellular sensitivity to epirubicin. Furthermore, the analysis of apoptosis related protein revealed that up-regulation of Id-1 suppressed epirubicin-induced apoptosis and down-regulation of Id-1 leading to increased epirubicin-induced apoptosis. Wound closure assay showed up-regulation of Id-1 leading to improved migration abilities of bladder cancer cells under chemotherapy. Our results suggest that up-regulation of Id-1 in bladder cancer cells lead to increased cell viability in response to epirubicin by its improved anti-apoptotic role, and down-regulation of Id-1 increases cellular sensitivity to epirubicin by increased anticancer drug-induced apoptosis.
Hu
Hao
H
Department of Urology, Peking University People's Hospital, Peking University Health Science Center, Beijing 100044, China.
Han
Hui Ying
HY
Wang
Yu Liang
YL
Zhang
Xiao Peng
XP
Chua
Chee Wai
CW
Wong
Y C
YC
Wang
Xiao Feng
XF
Ling
Ming Tat
MT
Xu
Ke Xin
KX
D000903:Antibiotics, Antineoplastic / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects; D015251:Epirubicin / Q000494:pharmacology*; D006801:Humans; D051798:Inhibitor of Differentiation Protein 1 / Q000032:analysis / Q000502:physiology*; D014162:Transfection; D001749:Urinary Bladder Neoplasms / Q000188:drug therapy* / Q000473:pathology
D000903:Antibiotics, Antineoplastic; C497453:ID1 protein, human; D051798:Inhibitor of Differentiation Protein 1; D015251:Epirubicin