title: SIRT1 regulates tyrosine hydroxylase expression and differentiation of neuroblastoma cells via FOXO3a.
abstract: To examine the function of SIRT1 in neuronal differentiation, we employed all-trans retinoic acid (ATRA)-induced differentiation of neuroblastoma cells. Nicotinamide inhibited neurite outgrowth and tyrosine hydroxylase (TH) expression. Inhibition of PARP or histone deacetylase did not inhibit TH expression, showing the effect to be SIRT1 specific. Expression of FOXO3a and its target proteins were increased during the differentiation and reduced by nicotinamide. FOXO3a deacetylation was increased by ATRA and blocked by nicotinamide. SIRT1 and FOXO3a siRNA inhibited ATRA-induced up-regulation of TH and differentiation. Taken together, these results indicate that SIRT1 is involved in ATRA-induced differentiation of neuroblastoma cells via FOXO3a.
authors.affiliation: Department of Anatomy and Neurobiology, College of Medicine, Hallym University, Chuncheon, Gangwon-do 200-702, South Korea.
authors.forename: Min-Ju
authors.initials: MJ
authors.lastname: Kim
authors.forename: Kyungsook
authors.initials: K
authors.lastname: Ahn
authors.forename: Seong-Hoon
authors.initials: SH
authors.lastname: Park
authors.forename: Hong-Jun
authors.initials: HJ
authors.lastname: Kang
authors.forename: Bong Geom
authors.initials: BG
authors.lastname: Jang
authors.forename: Soo-Jin
authors.initials: SJ
authors.lastname: Oh
authors.forename: Sun-Mee
authors.initials: SM
authors.lastname: Oh
authors.forename: Yu-Jin
authors.initials: YJ
authors.lastname: Jeong
authors.forename: Jee-In
authors.initials: JI
authors.lastname: Heo
authors.forename: Jun-Gyo
authors.initials: JG
authors.lastname: Suh
authors.forename: Soon Sung
authors.initials: SS
authors.lastname: Lim
authors.forename: Yoon-Jung
authors.initials: YJ
authors.lastname: Ko
authors.forename: Sung-Oh
authors.initials: SO
authors.lastname: Huh
authors.forename: Sung Chan
authors.initials: SC
authors.lastname: Kim
authors.forename: Jae-Bong
authors.initials: JB
authors.lastname: Park
authors.forename: Jaebong
authors.initials: J
authors.lastname: Kim
authors.forename: Jong-Il
authors.initials: JI
authors.lastname: Kim
authors.forename: Sangmee Ahn
authors.initials: SA
authors.lastname: Jo
authors.forename: Jae-Yong
authors.initials: JY
authors.lastname: Lee
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology; D002454:Cell Differentiation; D045744:Cell Line, Tumor; D024161:Collagen Type XI / Q000378:metabolism; D000071316:Forkhead Box Protein O3; D051858:Forkhead Transcription Factors / Q000235:genetics / Q000378:metabolism*; D015971:Gene Expression Regulation, Enzymologic; D015972:Gene Expression Regulation, Neoplastic; D006655:Histone Deacetylases / Q000378:metabolism; D006801:Humans; D009363:Neoplasm Proteins / Q000235:genetics / Q000378:metabolism*; D009447:Neuroblastoma / Q000378:metabolism* / Q000473:pathology; D009474:Neurons / Q000378:metabolism / Q000473:pathology; D009536:Niacinamide / Q000494:pharmacology; D056564:Sirtuin 1; D037761:Sirtuins / Q000235:genetics / Q000378:metabolism*; D014212:Tretinoin / Q000494:pharmacology; D014446:Tyrosine 3-Monooxygenase / Q000096:biosynthesis* / Q000235:genetics; D014803:Vitamin B Complex / Q000494:pharmacology
chemical_list: D000970:Antineoplastic Agents; C499319:COL11A2 protein, human; D024161:Collagen Type XI; C499517:FOXO3 protein, human; D000071316:Forkhead Box Protein O3; D051858:Forkhead Transcription Factors; D009363:Neoplasm Proteins; D014803:Vitamin B Complex; D009536:Niacinamide; D014212:Tretinoin; D014446:Tyrosine 3-Monooxygenase; C447939:SIRT1 protein, human; D056564:Sirtuin 1; D037761:Sirtuins; D006655:Histone Deacetylases