title: Discovery and characterization of novel vascular and hematopoietic genes downstream of etsrp in zebrafish.
abstract: The transcription factor Etsrp is required for vasculogenesis and primitive myelopoiesis in zebrafish. When ectopically expressed, etsrp is sufficient to induce the expression of many vascular and myeloid genes in zebrafish. The mammalian homolog of etsrp, ER71/Etv2, is also essential for vascular and hematopoietic development. To identify genes downstream of etsrp, gain-of-function experiments were performed for etsrp in zebrafish embryos followed by transcription profile analysis by microarray. Subsequent in vivo expression studies resulted in the identification of fourteen genes with blood and/or vascular expression, six of these being completely novel. Regulation of these genes by etsrp was confirmed by ectopic induction in etsrp overexpressing embryos and decreased expression in etsrp deficient embryos. Additional functional analysis of two newly discovered genes, hapln1b and sh3gl3, demonstrates their importance in embryonic vascular development. The results described here identify a group of genes downstream of etsrp likely to be critical for vascular and/or myeloid development.
authors.affiliation: Department of Molecular, Cell and Developmental Biology, University of California Los Angeles, Los Angeles, California, United States of America.
authors.forename: Gustavo A
authors.initials: GA
authors.lastname: Gomez
authors.forename: Matthew B
authors.initials: MB
authors.lastname: Veldman
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhao
authors.forename: Shawn
authors.initials: S
authors.lastname: Burgess
authors.forename: Shuo
authors.initials: S
authors.lastname: Lin
mesh_terms: D000818:Animals; D001808:Blood Vessels / Q000254:growth & development; D004625:Embryo, Nonmammalian; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D006410:Hematopoiesis / Q000235:genetics*; D018919:Neovascularization, Physiologic / Q000235:genetics*; D009419:Nerve Tissue Proteins / Q000235:genetics* / Q000502:physiology; D011509:Proteoglycans / Q000235:genetics* / Q000502:physiology; D014157:Transcription Factors / Q000235:genetics* / Q000502:physiology; D015027:Zebrafish; D029961:Zebrafish Proteins / Q000235:genetics* / Q000502:physiology; D000099249:Proteoglycan Link Protein
chemical_list: D009419:Nerve Tissue Proteins; D011509:Proteoglycans; D014157:Transcription Factors; D029961:Zebrafish Proteins; C509408:Etv2 protein, zebrafish; C541687:Hapln1b protein, zebrafish; D000099249:Proteoglycan Link Protein; C541686:sh3gl3b protein, zebrafish