Screening Soybean for Resistance to Heterodera glycines Ichinohe Using Monoxenic Cultures.
A simple, rapid, and inexpensive method for evaluation of host-parasite interactions, based on monoxenic cultures, is described. Axenic root explants of Glycine max (L.) Merr., cultured on a holidic agar medium, were inoculated with axenic second-stage larvae of Heterodera glycines Ichinohe, Race 3. A clear separation of susceptible and resistant cultivars, based on numbers of mature female nematodes present after 3 wk at 25 C, was observed. The method described should aid researchers in the evaluation of the host response to infection by H. glycines.
Lauritis
J A
JA
Rebois
R V
RV
Graney
L S
LS