title: ST2 deficient mice display a normal host defense against pulmonary infection with Mycobacterium tuberculosis.
abstract: Tuberculosis, caused by Mycobacterium (M.) tuberculosis, is a devastating infectious disease causing many deaths world-wide every year. Successful host defense mainly depends on a strong Th type 1 response. We investigated the role of T1/ST2 (recently identified as the receptor for IL-33), a typical Th2 marker in the assumption that a shift towards a beneficial Th1 response would occur in the absence of ST2. For this, ST2 KO and WT mice were intranasally infected with a virulent strain of M. tuberculosis (150 CFU). In line with our hypothesis, ST2 KO animals displayed increased numbers of lymphocytes infiltrating the lung after 2 weeks of infection, increased IFNgamma production by splenocytes in ST2 KO mice early in infection and enhanced lung IFNgamma levels at the chronic phase of the disease. However, we did not detect any differences between ST2 KO and WT mice in mycobacterial loads in lungs or liver after M. tuberculosis infection. The pulmonary inflammatory response, as measured by relative lung weights, cytokine and chemokine levels as well as histopathological analysis, was similar in ST2 KO and WT mice. These data suggest that apart from inducing a modest shift towards the Th1 response, the role of ST2 during murine M. tuberculosis infection is limited.
authors.affiliation: Center of Infection and Immunity Amsterdam (CINIMA), Meibergdreef 9, Amsterdam, The Netherlands. c.wieland@amc.uva.nl
authors.forename: Catharina W
authors.initials: CW
authors.lastname: Wieland
authors.forename: Gerritje J W
authors.initials: GJ
authors.lastname: van der Windt
authors.forename: Sandrine
authors.initials: S
authors.lastname: Florquin
authors.forename: Andrew N J
authors.initials: AN
authors.lastname: McKenzie
authors.forename: Tom
authors.initials: T
authors.lastname: van der Poll
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D015169:Colony Count, Microbial; D005260:Female; D007371:Interferon-gamma / Q000032:analysis; D000072179:Interleukin-1 Receptor-Like 1 Protein; D007963:Leukocytes, Mononuclear / Q000276:immunology; D008099:Liver / Q000382:microbiology; D008168:Lung / Q000737:chemistry / Q000382:microbiology / Q000473:pathology; D008297:Male; D008565:Membrane Proteins / Q000172:deficiency / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D018345:Mice, Knockout; D009169:Mycobacterium tuberculosis / Q000276:immunology*; D018123:Receptors, Interleukin; D013154:Spleen / Q000276:immunology; D018417:Th1 Cells / Q000276:immunology*; D014397:Tuberculosis, Pulmonary / Q000276:immunology*
chemical_list: C486189:Il1rl1 protein, mouse; D000072179:Interleukin-1 Receptor-Like 1 Protein; D008565:Membrane Proteins; D018123:Receptors, Interleukin; D007371:Interferon-gamma