Brefeldin A inhibits expression of DNA packaging proteins and nucleocapsid formation of human cytomegalovirus.
In this study we used the fungal antibiotic brefeldin A (BFA) to analyze its effect on viral replication. Analysis by electron microscopy demonstrated that no viral particles were observed in cells treated before the onset of viral replication. In the presence of BFA expression of IE2, MCP, pUL104, pUL56 and pUL89 were reduced, while no or slight effect was observed on expression of pp65, pUL44 and pUL57. Strikingly, real time PCR revealed that de novo viral DNA synthesis is reduced but not completely abolished in the presence of BFA. These results indicated that BFA represents a multi-functional compound leading to inhibition of several steps of viral maturation such as expression of viral DNA packaging proteins and capsid formation.
Kregler
Oliver
O
Institute of Virology, Helmut-Ruska Haus, Charité Campus Mitte, Charité-Universitätsmedizin Berlin, Berlin, Germany.
Schilf
Rita
R
Lander
Angelika
A
Bannert
Norbert
N
Bogner
Elke
E
D000935:Antifungal Agents / Q000494:pharmacology*; D020126:Brefeldin A / Q000494:pharmacology*; D003587:Cytomegalovirus / Q000378:metabolism*; D005347:Fibroblasts; D005786:Gene Expression Regulation / Q000187:drug effects*; D006801:Humans; D008297:Male; D019251:Nucleocapsid / Q000378:metabolism*; D014764:Viral Proteins / Q000096:biosynthesis*; D014779:Virus Replication / Q000187:drug effects*
D000935:Antifungal Agents; D014764:Viral Proteins; D020126:Brefeldin A