title: False-negative Histoplasma antigen in acute pulmonary histoplasmosis: the value of urinary concentration by ultrafiltration and heat denaturation of serum proteins in detection of Histoplasma antigen.
abstract: We report an infant with localized pulmonary histoplasmosis in whom Histoplasma antibody assays, quantitative Histoplasma urine and serum antigen concentrations, and histopathologic findings of a mediastinal mass were nondiagnostic. A provisional diagnosis of histoplasmosis was established by using laboratory methods that increase the sensitivity of the antigen assay using ultrafiltration of urine and ethylenediaminetetraacetic acid/heat denaturation of serum proteins.
authors.affiliation: Department of Oncology, St. Jude Children's Research Hospital, Memphis, TN 38105, USA. ashok.srinivasan@stjude.org
authors.forename: Ashok
authors.initials: A
authors.lastname: Srinivasan
authors.forename: Martin B
authors.initials: MB
authors.lastname: Kleiman
authors.forename: Larisa
authors.initials: L
authors.lastname: Debelenko
authors.forename: Dennis C
authors.initials: DC
authors.lastname: Stokes
authors.forename: John
authors.initials: J
authors.lastname: De Vincenzo
authors.forename: Joseph L
authors.initials: JL
authors.lastname: Wheat
mesh_terms: D000893:Anti-Inflammatory Agents / Q000627:therapeutic use; D000935:Antifungal Agents / Q000627:therapeutic use; D000946:Antigens, Fungal / Q000302:isolation & purification* / Q000652:urine; D006658:Histoplasma / Q000302:isolation & purification*; D006660:Histoplasmosis / Q000175:diagnosis / Q000188:drug therapy / Q000382:microbiology*; D006358:Hot Temperature; D006801:Humans; D007223:Infant; D017964:Itraconazole / Q000627:therapeutic use; D008172:Lung Diseases, Fungal / Q000188:drug therapy / Q000382:microbiology*; D008297:Male; D011241:Prednisone / Q000627:therapeutic use; D011489:Protein Denaturation; D012680:Sensitivity and Specificity; D014462:Ultrafiltration / Q000379:methods
chemical_list: D000893:Anti-Inflammatory Agents; D000935:Antifungal Agents; D000946:Antigens, Fungal; D017964:Itraconazole; D011241:Prednisone