title: [In vitro observation on albendazole sulfoxide and its enantiomers against Echinococcus granulosus protoscolex].
abstract: OBJECTIVE
  To investigate in vitro anti-hydatid efficacy on Echinococcus granulosus protoscolex (EgPSC) by using albendazole sulfoxide (ASOX) and its two enantiomeric antipodes, L-ASOX and D-ASOX.
  
  
  METHODS
  Eg protoscoleces were divided into eight groups and cultured in the DMEM culture media under two concentrations(50 microg/ml and 100 microg/ml) of ASOX, L-ASOX and D-ASOX respectively. The appropriate controls included (i) a culture containing an equal amount of DMSO and (ii) a culture medium alone. The mortality of EgPSC in each group was daily counted until 100% EgPSC death in some groups.
  
  
  RESULTS
  Significant difference of EgPSC mortality was found among the three drugs with various concentrations compared to control group (P < 0.01), and a significant difference between L-ASOX group and D-ASOX group (P < 0.05). There were no statistical difference between ASOX group and D-ASOX group (P > 0.05), but between ASOX group and L-ASOX group (P < 0.05). On the 9th day of culture, the mortality of protoscoleces with the concentration of 50 microg/ml was 93.6%, 56.2% and 99.0% in ASOX, L-ASOX and D-ASOX groups respectively, and those under the concentration of 100 microg/ml were 100%, 74.5% and 100% respectively. The mortality was 19.1% and 22.5% respectively in the control and solvent groups.
  
  
  CONCLUSIONS
  ASOX, L-ASOX and D-ASOX demonstrate significant effect of anti- Eg protoscolex in vitro. D-ASOX shows stronger effect than L-ASOX.
authors.affiliation: Research Institute of Pathogenic Biology, School of Basic Medicine, Lanzhou University, Lanzhou 730000, China. baogsh@lzu.edu.cn
authors.forename: Gen-shu
authors.initials: GS
authors.lastname: Bao
authors.forename: Hong
authors.initials: H
authors.lastname: Zhang
authors.forename: Tao
authors.initials: T
authors.lastname: Jing
authors.forename: Hua
authors.initials: H
authors.lastname: Qiao
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
mesh_terms: D015766:Albendazole / Q000031:analogs & derivatives* / Q000737:chemistry / Q000494:pharmacology; D000818:Animals; D000871:Anthelmintics / Q000737:chemistry / Q000494:pharmacology*; D048209:Echinococcus granulosus / Q000187:drug effects*
chemical_list: D000871:Anthelmintics; D015766:Albendazole; C027186:albendazole sulfoxide