Proteases from salmon stimulate IL-8 in airway epithelial cells.
In this study the ability of salmon tissue extracts to stimulate interleukin 8 (IL-8) production in airway epithelial cells (A549) was investigated; in particular, the role of serine protease enzymes and endotoxin was examined with respect to IL-8-stimulating ability. A549 cells were stimulated by various concentrations of fish tissue extracts for 6 h. Parallel samples were incubated with a protease inhibitor cocktail, a serine protease inhibitor, or an endotoxin inhibitor. The amount of secreted IL-8 in the supernatant was determined by an enzyme-linked immunosorbent assay. A549 cells showed a concentration-dependent increase in IL-8 secretion after stimulation with extracts of salmon tissues. The IL-8-stimulating effect was inhibited by serine protease inhibitors but not by endotoxin inhibitors.
Bang
Berit
B
Department of Occupational and Environmental Medicine, University Hospital of North Norway, Tromsø, Norway. berit.bang@unn.no
Larsen
Merethe
M
Larsen
Anett Kristin
AK
Aasmoe
Lisbeth
L
D000818:Animals; D045744:Cell Line, Tumor; D004731:Endotoxins / Q000037:antagonists & inhibitors; D004847:Epithelial Cells / Q000187:drug effects* / Q000378:metabolism; D006801:Humans; D016209:Interleukin-8 / Q000378:metabolism*; D011112:Polymyxin B / Q000494:pharmacology; D011480:Protease Inhibitors / Q000494:pharmacology; D020545:Respiratory Mucosa / Q000187:drug effects* / Q000378:metabolism; D012474:Salmon / Q000378:metabolism*; D012697:Serine Endopeptidases / Q000494:pharmacology*; D015842:Serine Proteinase Inhibitors / Q000494:pharmacology; D014020:Tissue Extracts / Q000494:pharmacology
D004731:Endotoxins; D016209:Interleukin-8; D011480:Protease Inhibitors; D015842:Serine Proteinase Inhibitors; D014020:Tissue Extracts; D012697:Serine Endopeptidases; D011112:Polymyxin B