title: Micellar lipid composition profoundly affects LXR-dependent cholesterol transport across CaCo2 cells.
abstract: Intraluminal phospholipids affect micellar solubilization and absorption of cholesterol. We here study cholesterol transport from taurocholate-phospholipid-cholesterol micelles to CaCo2 cells, and associated effects on ABC-A1 mediated cholesterol efflux. Micellar incorporation of egg-yolk-phosphatidylcholine markedly increased apical retention of the sterol with decreased expression of ABC-A1, an effect that is prevented by synthetic liver X receptor (LXR) or retinoid X receptor (RXR) agonists. On the other hand, incorporation of lyso-phosphatidylcholine (LysoPC) increased ABC-A1-HDL-dependent basolateral cholesterol efflux, an effect that is abated when LXR is silenced. Thus, the modulation of cholesterol metabolism via intraluminal phospholipids is related to the activity of the oxysterol nuclear receptor LXR.
authors.affiliation: Department of Experimental Hepatology, Academic Medical Center, Amsterdam, The Netherlands.
authors.forename: Michele
authors.initials: M
authors.lastname: Petruzzelli
authors.forename: Albert K
authors.initials: AK
authors.lastname: Groen
authors.forename: Karel J
authors.initials: KJ
authors.lastname: van Erpecum
authors.forename: Carlos
authors.initials: C
authors.lastname: Vrins
authors.forename: Astrid E
authors.initials: AE
authors.lastname: van der Velde
authors.forename: Piero
authors.initials: P
authors.lastname: Portincasa
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Palasciano
authors.forename: Gerard P
authors.initials: GP
authors.lastname: van Berge Henegouwen
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Lo Sasso
authors.forename: Annalisa
authors.initials: A
authors.lastname: Morgano
authors.forename: Antonio
authors.initials: A
authors.lastname: Moschetta
mesh_terms: D001692:Biological Transport; D018938:Caco-2 Cells; D002784:Cholesterol / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D050356:Lipid Metabolism; D000071518:Liver X Receptors; D008823:Micelles; D057093:Orphan Nuclear Receptors; D012333:RNA, Messenger / Q000235:genetics; D018160:Receptors, Cytoplasmic and Nuclear / Q000235:genetics / Q000378:metabolism*
chemical_list: D004268:DNA-Binding Proteins; D000071518:Liver X Receptors; D008823:Micelles; D057093:Orphan Nuclear Receptors; D012333:RNA, Messenger; D018160:Receptors, Cytoplasmic and Nuclear; D002784:Cholesterol