title: Tryptophan 13C nuclear-spin polarization generated by intraprotein electron transfer in a LOV2 domain of the blue-light receptor phototropin.
abstract: (13)C-NMR experiments were performed on photo-excited fully and partially (13)C-labelled LOV2 domains of the blue-light receptor phototropin. In the present paper, we report on nuclear-spin polarized tryptophan resonances that are generated by light-induced intraprotein electron transfer to the FMN cofactor. The spectra are discussed with respect to earlier data obtained from (13)C-NMR experiments on unlabelled LOV2 domains that have been reconstituted with FMN (13)C isotopologues.
authors.affiliation: Department Chemie, Technische Universität München, Garching, Germany.
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Eisenreich
authors.forename: Markus
authors.initials: M
authors.lastname: Fischer
authors.forename: Werner
authors.initials: W
authors.lastname: Römisch-Margl
authors.forename: Monika
authors.initials: M
authors.lastname: Joshi
authors.forename: Gerald
authors.initials: G
authors.lastname: Richter
authors.forename: Adelbert
authors.initials: A
authors.lastname: Bacher
authors.forename: Stefan
authors.initials: S
authors.lastname: Weber
mesh_terms: D002247:Carbon Isotopes; D056931:Cryptochromes; D004579:Electron Transport; D005486:Flavin Mononucleotide / Q000737:chemistry / Q000528:radiation effects; D005420:Flavoproteins / Q000737:chemistry* / Q000528:radiation effects; D008027:Light; D009682:Magnetic Resonance Spectroscopy; D017434:Protein Structure, Tertiary; D014364:Tryptophan / Q000737:chemistry*
chemical_list: D002247:Carbon Isotopes; D056931:Cryptochromes; D005420:Flavoproteins; D005486:Flavin Mononucleotide; D014364:Tryptophan