Isolation of Pseudomonas stutzeri in wastewater of catfish fish-ponds in the Mekong Delta and its application for wastewater treatment.
The aim of this study was to explore the potential for reducing soluble N load in fishpond wastewater using naturally occurring denitrifying bacteria. Twenty-seven isolates were selected from in wastewater (liquid/solid) of catfish-ponds located along the Tien river, in the Mekong Delta, Vietnam in SW-LB medium (artificial seawater Luria-Britani medium) supplemented with 10 mM NH4 and NO3 and twenty-five isolates were identified as Pseudomonas stutzeri based on similarity of PCR-16S rRNA using universal primers and specific primers. Four isolates were effective in lowering soluble N (NH4, NO2 and NO3) levels in fishpond water from 10 mg/L to negligible amounts after four days. Further experiments are underway to determine the fate of N lost from solution and the relative activity of ammonia oxidation, and nitrite and nitrate reduction by P. stutzeri isolates.
Diep
Cao Ngoc
CN
Microbiology Department, Biotechnology R&D Institute, Can Tho University, Can Tho City, Vietnam. cndiep@ctu.edu.vn
Cam
Pham My
PM
Vung
Nguyen Hoai
NH
Lai
To Thi
TT
My
Nguyen Thi Xuan
NT
D017756:Aquaculture; D017931:DNA Primers / Q000235:genetics; D009584:Nitrogen / Q000378:metabolism; D016133:Polymerase Chain Reaction; D044223:Pseudomonas stutzeri / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D045483:Rivers; D014744:Vietnam; D014865:Waste Disposal, Fluid / Q000379:methods*; D014874:Water Pollutants, Chemical / Q000378:metabolism*; D018508:Water Purification / Q000379:methods*
D017931:DNA Primers; D012336:RNA, Ribosomal, 16S; D014874:Water Pollutants, Chemical; D009584:Nitrogen