title: Transduction of nonhuman primate brain with adeno-associated virus serotype 1: vector trafficking and immune response.
abstract: We used convection-enhanced delivery (CED) to characterize gene delivery mediated by adeno-associated virus type 1 (AAV1) by tracking expression of hrGFP (humanized green fluorescent protein from Renilla reniformis) into the striatum, basal forebrain, and corona radiata of monkey brain. Four cynomolgus monkeys received single infusions into corona radiata, putamen, and caudate. The other group (n = 4) received infusions into basal forebrain. Thirty days after infusion animals were killed and their brains were processed for immunohistochemical evaluation. Volumetric analysis of GFP-positive brain areas was performed. AAV1-hrGFP infusions resulted in approximately 550, 700, and 73 mm(3) coverage after infusion into corona radiata, striatum, and basal forebrain, respectively. Aside from targeted regions, other brain structures also showed GFP signal (internal and external globus pallidus, subthalamic nucleus), supporting the idea that AAV1 is actively trafficked to regions distal from the infusion site. In addition to neuronal transduction, a significant nonneuronal cell population was transduced by AAV1 vector; for example, oligodendrocytes in corona radiata and astrocytes in the striatum. We observed a strong humoral and cell-mediated response against AAV1-hrGFP in transduced monkeys irrespective of the anatomic location of the infusion, as evidenced by induction of circulating anti-AAV1 and anti-hrGFP antibodies, as well as infiltration of CD4(+) lymphocytes and upregulation of MHC-II in regions infused with vector. We conclude that transduction of antigen-presenting cells within the CNS is a likely cause of this response and that caution is warranted when foreign transgenes are used as reporters in gene therapy studies with vectors with broader tropism than AAV2.
authors.affiliation: Laboratory of Molecular Therapeutics, Department of Neurosurgery, University of California-San Francisco, San Francisco, CA 94103, USA. piotr.hadaczek@ucsf.edu
authors.forename: Piotr
authors.initials: P
authors.lastname: Hadaczek
authors.forename: John
authors.initials: J
authors.lastname: Forsayeth
authors.forename: Hanna
authors.initials: H
authors.lastname: Mirek
authors.forename: Keith
authors.initials: K
authors.lastname: Munson
authors.forename: John
authors.initials: J
authors.lastname: Bringas
authors.forename: Phil
authors.initials: P
authors.lastname: Pivirotto
authors.forename: Jodi L
authors.initials: JL
authors.lastname: McBride
authors.forename: Beverly L
authors.initials: BL
authors.lastname: Davidson
authors.forename: Krystof S
authors.initials: KS
authors.lastname: Bankiewicz
mesh_terms: D000818:Animals; D000917:Antibody Formation; D001253:Astrocytes / Q000378:metabolism; D000229:Dependovirus / Q000235:genetics / Q000276:immunology*; D015316:Genetic Therapy / Q000009:adverse effects / Q000379:methods; D005822:Genetic Vectors / Q000008:administration & dosage / Q000276:immunology* / Q000378:metabolism*; D049452:Green Fluorescent Proteins; D006801:Humans; D007263:Infusions, Parenteral; D008252:Macaca fascicularis; D009422:Nervous System Diseases / Q000276:immunology / Q000378:metabolism / Q000628:therapy; D009474:Neurons / Q000378:metabolism; D009836:Oligodendroglia / Q000378:metabolism; D016548:Prosencephalon / Q000166:cytology / Q000276:immunology* / Q000378:metabolism*; D011994:Recombinant Proteins / Q000096:biosynthesis; D014161:Transduction, Genetic / Q000379:methods*
chemical_list: D011994:Recombinant Proteins; D049452:Green Fluorescent Proteins