Interactive effects of sulfur and nitrogen supply on the concentration of sinigrin and allyl isothiocyanate in Indian mustard (Brassica juncea L.).
Food derived from Brassica species is rich in glucosinolates. Hydrolysis of these compounds by myrosinase yields isothiocyanates and other breakdown products, which due to their pungency represent the primary purpose of Indian mustard cultivation. Strong interactive effects of S (0.0, 0.2, and 0.6 g pot(-1)) and N (1, 2, and 4 g pot(-1)) supply on growth, seed yield, and the concentrations of glucosinolates and isothiocyanates in seeds were observed in growth experiments, reflecting the involvement of S-containing amino acids in both protein and glucosinolate synthesis. At intermediate S supply, a strong N-induced S limitation was apparent, resulting in high concentrations of sinigrin (12 micromol g(-1) of DM) and allyl isothiocyanate (213 micromol kg(-1) of DM) at low N supply only. Myrosinase activity in seeds increased under low N and low S supply, but the results do not suggest that sinigrin functions as a transient reservoir for S.
Gerendás
Jóska
J
Institute for Plant Nutrition and Soil Science, Hermann-Rodewald Strasse 2, University of Kiel, D-24098 Kiel, Germany.
Podestát
Jana
J
Stahl
Thorsten
T
Kübler
Kerstin
K
Brückner
Hans
H
Mersch-Sundermann
Volker
V
Mühling
Karl H
KH
D004347:Drug Interactions; D005308:Fertilizers; D005961:Glucosinolates / Q000032:analysis*; D006026:Glycoside Hydrolases / Q000378:metabolism; D017879:Isothiocyanates / Q000032:analysis*; D009149:Mustard Plant / Q000737:chemistry / Q000187:drug effects / Q000254:growth & development*; D009584:Nitrogen / Q000008:administration & dosage*; D012639:Seeds / Q000737:chemistry* / Q000201:enzymology; D013455:Sulfur / Q000008:administration & dosage*
D005308:Fertilizers; D005961:Glucosinolates; D017879:Isothiocyanates; C010330:sinigrin; D013455:Sulfur; C004471:allyl isothiocyanate; D006026:Glycoside Hydrolases; C022739:thioglucosidase; D009584:Nitrogen