Comparison of three microbial screening methods for antibiotics using routine monitoring samples.
Monitoring large numbers of slaughter animals for the presence of antimicrobial residues is preferably carried out using microbiological screening methods, because of their high cost-effectiveness. An evaluation of the Nouws antibiotic test (NAT) was performed on routine monitoring samples and the performance of the method was compared with two other microbial screening methods: Screening test for antibiotic residues (STAR) and Premi Test. Analysis of 591 samples yielded four MRL violations. Three of them concerned tetracyclines that were only detected with the NAT and the STAR method. The fourth, 172 microgkg(-1) Sulfadiazine, was detected by all three methods. Additionally, 156 microgkg(-1) Tulathromycin was found in porcine meat, while for this residue no MRL in muscle has been established.
Pikkemaat
Mariël G
MG
RIKILT, Institute of Food Safety, Wageningen University and Research Centre, P.O. Box 230, 6700 AE Wageningen, The Netherlands.
Rapallini
Michel L B A
ML
Dijk
Sabrina Oostra-van
SO
Elferink
J W Alexander
JW
D000818:Animals; D000900:Anti-Bacterial Agents / Q000032:analysis*; D004350:Drug Residues / Q000032:analysis*; D005513:Food Inspection / Q000379:methods*; D008460:Meat / Q000032:analysis*; D008826:Microbial Sensitivity Tests / Q000379:methods*; D011933:Reagent Kits, Diagnostic; D013411:Sulfadiazine / Q000032:analysis; D013552:Swine; D013754:Tetracyclines / Q000032:analysis
D000900:Anti-Bacterial Agents; D011933:Reagent Kits, Diagnostic; D013754:Tetracyclines; D013411:Sulfadiazine