[Effects of yougui drink on the osteogenic differentiation of mesenchymal stem cells in the proximal femur in steroid-induced avascular necrosis of femoral head].
OBJECTIVE
To investigate the effects of Yougui drink on osteogenic differentiation of bone mesenchymal stem cells (BMSCs) in the proximal femur in steroid-induced avascular necrosis of femoral head in vitro.


METHODS
The BMSCs were induced to osteoblasts in vitro with a conditional medium. The cells cultured with Yougui drink serum were compared with the ordinary one, which both were examined by microscopy, MTU method, ALP and calcium node.


RESULTS
Microscopy and MTU studies showed that Yougui drink could improve proliferation of the osteoblasts. ALP and calcium node studies showed that Yougui drink could improve the activity of the osteoblasts (P < 0.01).


CONCLUSIONS
Yougui drink has positive effects on osteogenic differentiation of mesenchymal stem cells in the proximal femur in steroid-induced avascular necrosis of femoral head in vitro.
Wu
Yun-gang
YG
The First Hospital Affiliated to Wenzhou Medical College, Wenzhou 325000, Zhejiang, China. zjwyg@126.com
Xiao
Lu-wei
LW
Tong
Pei-jian
PJ
D002454:Cell Differentiation; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000494:pharmacology*; D005260:Female; D005271:Femur Head Necrosis / Q000188:drug therapy* / Q000503:physiopathology; D006801:Humans; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000187:drug effects; D008875:Middle Aged; D010006:Osteoblasts / Q000166:cytology* / Q000187:drug effects; D010020:Osteonecrosis; D013256:Steroids / Q000494:pharmacology*
D004365:Drugs, Chinese Herbal; D013256:Steroids