title: A novel cardiotropic murine adenovirus representing a distinct species of mastadenoviruses.
abstract: During cell culture isolation experiments to recover Dobrava hantavirus from a suspension of liver from a striped field mouse (Apodemus agrarius), an unknown virus was coisolated. Atypically for hantaviruses, it had extensive cytopathic effects. Using a random PCR approach, it was identified as a novel murine adenovirus, MAdV-3 (for MAdV type 3). A plaque-purified virus clone was prepared and further characterized. The complete genome sequence of MAdV-3 was determined to be 30,570 bp in length. Sequence comparisons to other adenovirus species revealed highest similarity to MAdV-1, the representative of the murine adenovirus A species. However, substantial differences were found in the E1, E3, and E4 genomic regions. The phylogenetic distance of MAdV-3 amino acid sequences for pVIII, protease, polymerase, and hexon from MAdV-1 is markedly higher than 0.1 exchange per position, and, based on our cross-neutralization experiments, MAdV-3 and MAdV-1 can be regarded as different serotypes. Therefore, we propose to classify MAdV-3 as the first isolate of a novel adenovirus species, designated murine adenovirus C (MAdV-C). The novel MAdV-3 virus is not only genetically and serologically distinct from MAdV-1 but also shows a unique organ tropism in infected mice. In contrast to MAdV-1, the virus was not detectable in brain but predominantly infected heart tissue. Thus, infection of mice with cardiotropic MAdV-3 might be an interesting animal model of adenovirus-induced myocarditis.
authors.affiliation: Institute of Virology, Helmut-Ruska-Haus, Charité University Hospital, Berlin, Germany.
authors.forename: Boris
authors.initials: B
authors.lastname: Klempa
authors.forename: Detlev H
authors.initials: DH
authors.lastname: Krüger
authors.forename: Brita
authors.initials: B
authors.lastname: Auste
authors.forename: Michal
authors.initials: M
authors.lastname: Stanko
authors.forename: Adalbert
authors.initials: A
authors.lastname: Krawczyk
authors.forename: Katrin F
authors.initials: KF
authors.lastname: Nickel
authors.forename: Klaus
authors.initials: K
authors.lastname: Uberla
authors.forename: Alexander
authors.initials: A
authors.lastname: Stang
mesh_terms: D000256:Adenoviridae / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D017398:Alternative Splicing / Q000235:genetics; D000818:Animals; D001483:Base Sequence; D002522:Chlorocebus aethiops; D004279:DNA, Viral / Q000235:genetics; D005260:Female; D016679:Genome, Viral / Q000235:genetics; D017921:Mastadenovirus / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D016366:Open Reading Frames / Q000235:genetics; D010802:Phylogeny; D016415:Sequence Alignment; D014709:Vero Cells
chemical_list: D004279:DNA, Viral