title: Comparison of antibody monitoring system with flow cytometric crossmatch test in renal transplant recipients with high panel-reactive antibody.
abstract: OBJECTIVE
  The antibody monitoring system (AMS) is a recently developed enzyme-linked immunosorbent assay (ELISA) crossmatch assay to detect donor-specific anti-HLA immunoglobulin G antibodies (DS-HLA Abs). This study was conducted to compare the AMS with the flow cytometric crossmatch (FCXM) test in renal transplant recipients with high panel-reactive antibody (PRA).
  
  
  METHODS
  Thirty-two sera were obtained from 10 patients with panel reactivity above 50%. When anti-HLA Ab was detected by ELISA PRA and the matched donor had the corresponding HLA antigen, it was considered to indicate DS-HLA Ab. The results of the AMS assay and FCXM were compared with the DS-HLA Abs.
  
  
  RESULTS
  Twenty-three (71.9%) sera were positive for DS-HLA Abs by ELISA PRA. The AMS assay showed that the number of compatible sera with DS-HLA Abs was 27 (84.4%), and it was significantly concordant (kappa = 0.649, p < 0.0001). For FCXM, the number of compatible sera with DS-HLA Abs was 26 (81.3%), and it was also significantly concordant (kappa = 0.614, p < 0.0001). There was a significant degree of concordance between the AMS assay and FCXM in detection of DS-HLA Abs (kappa = 0.452, p = 0.010).
  
  
  CONCLUSIONS
  The AMS assay is comparable to FCXM in detecting DS-HLA Abs in high PRA recipients.
authors.affiliation: Departments of Internal Medicine, Kangnam St. Mary's Hospital, The Catholic University of Korea, 505 Banpo-Dong, Seocho-Ku, Seoul, South Korea.
authors.forename: Hyeon Seok
authors.initials: HS
authors.lastname: Hwang
authors.forename: Bok Jin
authors.initials: BJ
authors.lastname: Hyoung
authors.forename: So Young
authors.initials: SY
authors.lastname: Lee
authors.forename: Youn Joo
authors.initials: YJ
authors.lastname: Jeon
authors.forename: Hye Eun
authors.initials: HE
authors.lastname: Yoon
authors.forename: Jin Young
authors.initials: JY
authors.lastname: Kim
authors.forename: Bum Soon
authors.initials: BS
authors.lastname: Choi
authors.forename: Eun Jee
authors.initials: EJ
authors.lastname: Oh
authors.forename: Yong Soo
authors.initials: YS
authors.lastname: Kim
authors.forename: Byung Kee
authors.initials: BK
authors.lastname: Bang
authors.forename: Chul Woo
authors.initials: CW
authors.lastname: Yang
mesh_terms: D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005434:Flow Cytometry / Q000379:methods*; D006681:HLA-D Antigens / Q000276:immunology*; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood*; D016030:Kidney Transplantation / Q000276:immunology*; D015166:Monitoring, Immunologic / Q000379:methods*
chemical_list: D006681:HLA-D Antigens; D007074:Immunoglobulin G