Molecularly imprinted polymer for analysis of zidovudine and stavudine in human serum by liquid chromatography-mass spectrometry.
A molecularly imprinted polymer (MIP) using zidovudine (AZT) as template and methacrylic acid as monomer was prepared. The synthesis of the MIP was performed in acetonitrile. The synthesized material was then tested for the solid-phase extraction of AZT from different media (pure organic solvents and hydro-organic mixtures). An optimised procedure was developed for the selective extraction of AZT with a recovery of 96% using the MIP and only 3% on a non-imprinted polymer used as control polymer. A specific capacity of 0.2 micromol g(-1) was determined. The specificity of the MIP was evaluated by studying the retention behaviour of two others nucleoside analogues. The feasibility of the MIP to selectively extract AZT and stavudine (d4T) from human serum was also demonstrated with recoveries of 80 and 85% respectively. The lower limit of quantification (LLOQ) and the lower limits of detection (LLOD) for AZT were 5.10(-7) and 10(-7) M respectively.
Duy
S Vo
SV
Institut des Biomolécules Max Mousseron (IBMM), UMR 5247 CNRS-UM1-UM2, Université Montpellier 2, Montpellier Cedex 05, France.
Lefebvre-Tournier
I
I
Pichon
V
V
Hugon-Chapuis
F
F
Puy
J-Y
JY
Périgaud
C
C
D019380:Anti-HIV Agents / Q000097:blood*; D002851:Chromatography, High Pressure Liquid; D003429:Cross Reactions; D006801:Humans; D007202:Indicators and Reagents; D013058:Mass Spectrometry; D011108:Polymers / Q000737:chemistry; D012997:Solvents; D018119:Stavudine / Q000097:blood*; D015215:Zidovudine / Q000097:blood*
D019380:Anti-HIV Agents; D007202:Indicators and Reagents; D011108:Polymers; D012997:Solvents; D015215:Zidovudine; D018119:Stavudine