title: 2-DE and LC-MS/MS for a comparative proteomic analysis of BALf from subjects with different subsets of inflammatory myopathies.
abstract: The protein profiles of bronchoalveolar lavage fluid (BALf) of patients belonging to three selected subsets of Polymyositis/Dermatomyositis (PM/DM) have been compared by using a combination of 2-DE and MALDI-TOF/MS or LC-MS/MS. Our study examined the hypothesis that there were distinct differences in protein expression profiles that were related to the phenotype. From among the 323+/-51 protein spots that may represent the most highly expressed proteins in BALf of these patients, 24 unique spots were isolated and proteins identified. In particular, 9 spots were present in BALf of PM/DM patients only; 12 spots were exclusive of Overlap patients and 3 spots of AS patients. From among the proteins identified, a few were classified as cytoskeletal proteins, others were involved in oxidative stress and a number of proteins were associated with general metabolic activity or immunological response and inflammation. This is the first study in which evidence is provided that a number of different proteins are expressed in different subsets of PM/DM and supports our contention that the proteomic approach would be beneficial in discovering molecules which could represent possible prognostic factors of these rare pathologies.
authors.affiliation: Department of Biochemistry A. Castellani, University of Pavia, Via Taramelli 3/B, 27100 Pavia, Italy.
authors.forename: Ileana
authors.initials: I
authors.lastname: Passadore
authors.forename: Paolo
authors.initials: P
authors.lastname: Iadarola
authors.forename: Cristina
authors.initials: C
authors.lastname: Di Poto
authors.forename: Serena
authors.initials: S
authors.lastname: Giuliano
authors.forename: Carlomaurizio
authors.initials: C
authors.lastname: Montecucco
authors.forename: Lorenzo
authors.initials: L
authors.lastname: Cavagna
authors.forename: Claudia
authors.initials: C
authors.lastname: Bonino
authors.forename: Federica
authors.initials: F
authors.lastname: Meloni
authors.forename: Anna Maria
authors.initials: AM
authors.lastname: Fietta
authors.forename: Antonella
authors.initials: A
authors.lastname: Lisa
authors.forename: Roberta
authors.initials: R
authors.lastname: Salvini
authors.forename: Anna Maria
authors.initials: AM
authors.lastname: Bardoni
mesh_terms: D000368:Aged; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry*; D002853:Chromatography, Liquid; D051338:Cofilin 1 / Q000097:blood; D003882:Dermatomyositis / Q000097:blood / Q000378:metabolism* / Q000473:pathology; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods*; D018260:Gelsolin / Q000097:blood; D006801:Humans; D015151:Immunoblotting; D008875:Middle Aged; D011506:Proteins / Q000032:analysis / Q000145:classification / Q000378:metabolism; D040901:Proteomics / Q000379:methods*; D015203:Reproducibility of Results; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D053719:Tandem Mass Spectrometry; D014746:Vimentin / Q000097:blood
chemical_list: D051338:Cofilin 1; D018260:Gelsolin; D011506:Proteins; D014746:Vimentin