Rat marrow-derived mesenchymal stem cells developed in a medium supplemented with the autologous versus bovine serum.
The controversial effect of autologous serum (AS) on human mesenchymal stem cells (MSC) was studied in rat MSC culture. Rat bone marrow cells were plated in a medium containing either FBS (fetal bovine serum) or AS were cultured to passage 3, during which the population doubling number (PDN) of both cultures were measured and compared statistically. The number of viable cells, the cell colonogic activity, and cell growth rate were also compared. In addition, mineralization in the osteogenic cultures from each system was measured. Our data indicated that AS enriched medium provided a microenvironment in which growth rate as well as bone differentiation of the isolated MSCs were significantly higher than in FBS enriched medium.
Eslaminejad
Mohamadreza Baghaban
MB
Stem Cell Department, Cell Sciences Research Center, Royan Institute, ACECR, Tehran, Iran. eslami@royaninstitute.org
Rouhi
Leila
L
Arabnajafi
Mahmood
M
Baharvand
Hossein
H
D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology* / Q000378:metabolism; D002113:Calcification, Physiologic / Q000502:physiology; D002417:Cattle; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D049109:Cell Proliferation; D003470:Culture Media / Q000737:chemistry*; D005260:Female; D006801:Humans; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D044967:Serum / Q000737:chemistry / Q000378:metabolism*; D013234:Stem Cells
D003470:Culture Media