title: Molecular characterizations of chloramphenicol- and oxytetracycline-resistant bacteria and resistance genes in mariculture waters of China.
abstract: In order to gain an understanding of the diversity and distribution of antimicrobial-resistant bacteria and their resistance genes in maricultural environments, multidrug-resistant bacteria were screened for the rearing waters from a mariculture farm of China. Both abalone Haliotis discushannai and turbot Scophthalmus maximus rearing waters were populated with abundant chloramphenicol-resistant bacteria. These bacteria were also multidrug resistant, with Vibriosplendidus and Vibriotasmaniensis being the most predominant species. The chloramphenicol-resistance gene cat II, cat IV or floR could be detected in most of the multidrug-resistant isolates, and the oxytetracycline-resistance gene tet(B), tet(D), tet(E) or tet(M) could also be detected for most of the isolates. Coexistence of chloramphenicol- and oxytetracycline-resistance genes partially explains the molecular mechanism of multidrug resistance in the studied maricultural environments. Comparative studies with different antimicrobial agents as the starting isolation reagents may help detect a wider diversity of the antimicrobial-resistant bacteria and their resistance genes.
authors.affiliation: Centre for Bioengineering and Biotechnology, China University of Petroleum (East China), 66 Changjiang West Road, Qingdao 266555, China. danghy@upc.edu.cn
authors.forename: Hongyue
authors.initials: H
authors.lastname: Dang
authors.forename: Jingyi
authors.initials: J
authors.lastname: Zhao
authors.forename: Linsheng
authors.initials: L
authors.lastname: Song
authors.forename: Mingna
authors.initials: M
authors.lastname: Chen
authors.forename: Yaqing
authors.initials: Y
authors.lastname: Chang
mesh_terms: D000818:Animals; D017756:Aquaculture; D001419:Bacteria / Q000145:classification / Q000187:drug effects* / Q000235:genetics*; D002681:China; D002702:Chloramphenicol Resistance / Q000235:genetics*; D015169:Colony Count, Microbial; D024881:Drug Resistance, Bacterial / Q000235:genetics*; D005798:Genes, Bacterial / Q000235:genetics*; D010118:Oxytetracycline / Q000494:pharmacology; D010802:Phylogeny; D012623:Seawater / Q000382:microbiology*
chemical_list: D010118:Oxytetracycline