Collagen scaffolds loaded with collagen-binding NGF-beta accelerate ulcer healing.
Studies have shown that exogenous nerve growth factor (NGF) accelerates ulcer healing, but the inefficient growth factor delivery system limits its clinical application. In this report, we found that the native human NGF-beta fused with a collagen-binding domain (CBD) could form a collagen-based NGF targeting delivery system, and the CBD-fused NGF-beta could bind to collagen membranes efficiently. Using the rabbit dermal ischemic ulcer model, we have found that this targeting delivery system maintains a higher concentration and stronger bioactivity of NGF-beta on the collagen membranes by promoting peripheral nerve growth. Furthermore, it enhances the rate of ulcer healing through accelerating the re-epithelialization of dermal ulcer wounds and the formation of capillary lumens within the newly formed tissue area. Thus, collagen membranes loaded with collagen-targeting human NGF-beta accelerate ulcer healing efficiently.
Sun
Wenjie
W
Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China.
Lin
Hang
H
Chen
Bing
B
Zhao
Wenxue
W
Zhao
Yannan
Y
Xiao
Zhifeng
Z
Dai
Jianwu
J
D000818:Animals; D003094:Collagen / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D007150:Immunohistochemistry; D008855:Microscopy, Electron, Scanning; D020932:Nerve Growth Factor / Q000378:metabolism*; D011485:Protein Binding; D011817:Rabbits; D012883:Skin Ulcer / Q000378:metabolism*; D014945:Wound Healing
D003094:Collagen; D020932:Nerve Growth Factor