Self-assembling peptide amphiphile-based nanofiber gel for bioresponsive cisplatin delivery.
The aim of this study is to develop a bioresponsive cisplatin (CDDP) delivery system with a self-assembling peptide amphiphile (PA) comprising a cell-adhesive matrix metalloproteinase-2 (MMP-2)-sensitive GTAGLIGQRGDS and a fatty acid. A biomimetic CDDP-PA gel was spontaneously formed upon incubating a mixture of CDDP and the PA for 5 h at 37 degrees C. CDDP-PA gel formation was confirmed by rheological analysis. The structure of self-assembled CDDP-PA nanofibers inside the gel was determined by transmission electron microscopy (TEM). Bioresponsive drug release from the biomimetic gel was demonstrated by in vitro MMP-2-triggered CDDP release. The MMP-2-sensitive CDDP release was dependent on the enzyme concentration in the medium. Enzymatic degradation of the CDDP-PA gel was confirmed by TEM images of the gel degraded in an MMP-2 containing medium. The MMP-2-triggered CDDP release as well as the presentation of RGDS in the gel would potentially provide a spatially and temporally controlled delivery system for targeted anticancer drug delivery.
Kim
Jin-Ki
JK
Department of Pharmaceutics, The University of Mississippi, MS 38677, USA.
Anderson
Joel
J
Jun
Ho-Wook
HW
Repka
Michael A
MA
Jo
Seongbong
S
D002945:Cisplatin / Q000737:chemistry*; D016503:Drug Delivery Systems / Q000379:methods*; D020778:Matrix Metalloproteinase 2 / Q000737:chemistry; D046529:Microscopy, Electron, Transmission; D015394:Molecular Structure; D036103:Nanotechnology / Q000379:methods; D010455:Peptides / Q000737:chemistry*
D010455:Peptides; D020778:Matrix Metalloproteinase 2; D002945:Cisplatin