[Oxidative damage of gasoline engine exhausts to rat lung tissues].
OBJECTIVE
To study the effects of extracts of condensate, particulates and semivolatile organic compounds from gasoline engine exhaust on DNA damage, 8-oxoguanine DNA glycosylase-1 (OGG1) expression, and changes of ultra-structures in lungs of rats.


METHODS
Organic extracts of gasoline engine exhaust (GEE) was intratrachealy instilled into rat lungs at 0, 5.6, 16.7, and 50.0 L/kg body weight, respectively, once a week for a month. The single DNA strand break was measured by comet assay. The OGG1 was determined using immunohistochemistry method. The ultrastructure of lung cells was observed with electronic microscope.


RESULTS
The rates of tailed cells detected by the comet assay increased significantly when the rats were exposed to 16.7 and 50.0 L/kg of GEE compared with those exposed to solvent only (P < 0.05). However, the tail length did not differ significantly between the groups. Similarly, exposure to 16.7 and 50.0 L/kg of GEE led to increased OGG1 significantly. Significant changes of mitochondria in type I and II alveolar cells as well as respiratory bronchiole epithelial cells were observed, which included decrease of numbers, pyknosis and swelling.


CONCLUSIONS
Gasoline engine exhausts induce single DNA strand break, increase OGG1 expression, decrease numbers of mitochondria, and destroy ultrastructures of mitochondria in various lung cells of rats.
Che
Wang-Jun
WJ
Department of Environmental Health, West China School of Public Health, Sichuan University, Chengdu 610041, China.
Wang
Ling
L
Luo
Qing-Ying
QY
Wu
Mei
M
Zhang
Zun-Zhen
ZZ
D056809:Alveolar Epithelial Cells / Q000648:ultrastructure; D000818:Animals; D004249:DNA Damage / Q000187:drug effects; D045647:DNA Glycosylases / Q000235:genetics / Q000378:metabolism; D005260:Female; D005742:Gasoline / Q000633:toxicity; D008168:Lung / Q000166:cytology / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology*; D008297:Male; D008928:Mitochondria / Q000648:ultrastructure; D018384:Oxidative Stress; D052638:Particulate Matter / Q000633:toxicity*; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D001335:Vehicle Emissions / Q000633:toxicity*
D005742:Gasoline; D052638:Particulate Matter; D001335:Vehicle Emissions; D045647:DNA Glycosylases; C483741:OGG1 protein, rat