SUnSET, a nonradioactive method to monitor protein synthesis.
We developed a nonradioactive fluorescence-activated cell sorting-based assay, called surface sensing of translation (SUnSET), which allows the monitoring and quantification of global protein synthesis in individual mammalian cells and in heterogeneous cell populations. We demonstrate here, using mouse dendritic and T cells as a model, that SUnSET offers a technical alternative to classical radioactive labeling methods for the study of mRNA translation and cellular activation.
Schmidt
Enrico K
EK
Centre d'Immunologie de Marseille-Luminy, Université de la Méditerranée, France.
Clavarino
Giovanna
G
Ceppi
Maurizio
M
Pierre
Philippe
P
D020869:Gene Expression Profiling / Q000379:methods*; D015151:Immunoblotting / Q000379:methods*; D007553:Isotope Labeling; D014176:Protein Biosynthesis / Q000502:physiology*; D011506:Proteins / Q000032:analysis* / Q000378:metabolism*; D011691:Puromycin / Q000032:analysis*; D013194:Staining and Labeling / Q000379:methods
D011506:Proteins; D011691:Puromycin