Genotyping of IRGM tetranucleotide promoter oligorepeats by fluorescence resonance energy transfer.
Fluorescence resonance energy transfer (FRET) genotyping has been well established for the rapid assessment of single nucleotide polymorphisms (SNPs) and deletions. A design is presented that allows the typing of short tandem oligo repeat sequences using the LightTyper/LightCycler system. The protocol was evaluated and applied to the typing of a tetranucleotide promoter repeat of the human gene encoding the immunity-related GTPase family, M (IRGM) molecule in >2000 individuals from Ghana, West Africa.
Intemann
Christopher D
CD
Bernhard Nocht Institute for Tropical Medicine, Department of Molecular Medicine, Hamburg, Germany.
Thye
Thorsten
T
Sievertsen
Jürgen
J
Owusu-Dabo
Ellis
E
Horstmann
Rolf D
RD
Meyer
Christian G
CG
D001483:Base Sequence; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods*; D019204:GTP-Binding Proteins / Q000235:genetics*; D005838:Genotype; D005869:Ghana; D006801:Humans; D018895:Microsatellite Repeats; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic
D019204:GTP-Binding Proteins; C513539:IRGM protein, human