Improved detection of incipient vascular changes by a biotechnological platform combining post mortem MRI in situ with neuropathology.
The histopathological counterpart of white matter hyperintensities is a matter of debate. Methodological and ethical limitations have prevented this question to be elucidated. We want to introduce a protocol applying state-of-the-art methods in order to solve fundamental questions regarding the neuroimaging-neuropathological uncertainties comprising the most common white matter hyperintensities [WMHs] seen in aging. By this protocol, the correlation between signal features in in situ, post mortem MRI-derived methods, including DTI and MTR and quantitative and qualitative histopathology can be investigated. We are mainly interested in determining the precise neuroanatomical substrate of incipient WMHs. A major issue in this protocol is the exact co-registration of small lesion in a tridimensional coordinate system that compensates tissue deformations after histological processing. The protocol is based on four principles: post mortem MRI in situ performed in a short post mortem interval, minimal brain deformation during processing, thick serial histological sections and computer-assisted 3D reconstruction of the histological sections. This protocol will greatly facilitate a systematic study of the location, pathogenesis, clinical impact, prognosis and prevention of WMHs.
Grinberg
Lea Tenenholz
LT
Aging Brain Project, Department of Pathology, University of Sao Paulo Medical School, Av. Dr. Arnaldo 455, sala 1353-01246903, Sao Paulo, SP, Brazil. leagrinberg@usp.br
Amaro Junior
Edson
E
da Silva
Alexandre Valotta
AV
da Silva
Rafael Emidio
RE
Sato
João Ricardo
JR
dos Santos
Denis Dionizio
DD
de Paula Pacheco
Silmara
S
de Lucena Ferretti
Renata Eloah
RE
Paraizo Leite
Renata Elaine
RE
Pasqualucci
Carlos Augusto
CA
Teipel
Stefan J
SJ
Flatz
Wilhelm H
WH
Heinsen
Helmut
H
D000328:Adult; D000368:Aged; D000375:Aging / Q000473:pathology; D016880:Anisotropy; D001921:Brain / Q000473:pathology*; D002561:Cerebrovascular Disorders / Q000473:pathology*; D038524:Diffusion Magnetic Resonance Imaging; D005260:Female; D006801:Humans; D021621:Imaging, Three-Dimensional; D007150:Immunohistochemistry; D008279:Magnetic Resonance Imaging; D008297:Male; D008875:Middle Aged; D009413:Nerve Fibers, Myelinated / Q000473:pathology*