title: Increased expression of heat shock protein 105 in rat uterus of early pregnancy and its significance in embryo implantation.
abstract: BACKGROUND
  Heat shock proteins (Hsps) are a set of highly conserved proteins, Hsp105, has been suggested to play a role in reproduction.
  
  
  METHODS
  Spatio-temporal expression of Hsp105 in rat uterus during peri-implantation period was examined by immunohistochemistry and Western blot, pseudopregnant uterus was used as control. Injection of antisense oligodeoxynucleotides to Hsp105 into pregnant rat uteri was carried out to look at effect of Hsp105 on embryo implantation.
  
  
  RESULTS
  Expression of Hsp105 was mainly in the luminal epithelium on day 1 of pregnancy, and reached a peak level on day 5, whereas in stroma cells, adjacent to the implanting embryo, the strongest expression of Hsp105 was observed on day 6. The immunostaining profile in the uterus was consistent with that obtained by Western blot in the early pregnancy. In contrast, no obvious peak level of Hsp105 was observed in the uterus of pseudopregnant rat on day 5 or day 6. Furthermore, injection of antisense oligodeoxynucleotides to Hsp105 into the rat uterine horn on day 3 of pregnancy obviously suppressed the protein expression as expected and reduced number of the implanted embryos as compared with the control.
  
  
  CONCLUSIONS
  Temporal and spatial changes in Hsp105 expression in pregnant rat uterus may play a physiological role in regulating embryo implantation.
authors.affiliation: State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, PR China. yuanjx@ioz.ac.cn
authors.forename: Jin-Xiang
authors.initials: JX
authors.lastname: Yuan
authors.forename: Li-Juan
authors.initials: LJ
authors.lastname: Xiao
authors.forename: Cui-Ling
authors.initials: CL
authors.lastname: Lu
authors.forename: Xue-Sen
authors.initials: XS
authors.lastname: Zhang
authors.forename: Tao
authors.initials: T
authors.lastname: Liu
authors.forename: Min
authors.initials: M
authors.lastname: Chen
authors.forename: Zhao-Yuan
authors.initials: ZY
authors.lastname: Hu
authors.forename: Fei
authors.initials: F
authors.lastname: Gao
authors.forename: Yi-Xun
authors.initials: YX
authors.lastname: Liu
mesh_terms: D000818:Animals; D015153:Blotting, Western; D010064:Embryo Implantation / Q000187:drug effects / Q000235:genetics*; D005260:Female; D050957:HSP110 Heat-Shock Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D007150:Immunohistochemistry; D020319:Oligodeoxyribonucleotides, Antisense / Q000494:pharmacology; D011247:Pregnancy / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D014599:Uterus / Q000187:drug effects / Q000378:metabolism*
chemical_list: D050957:HSP110 Heat-Shock Proteins; C542230:Hsph1 protein, rat; D020319:Oligodeoxyribonucleotides, Antisense