Reduction of vitellogenin synthesis by an aryl hydrocarbon receptor agonist in the white sturgeon (Acipenser transmontamus).
Migrating white sturgeon (Acipenser transmontamus) may be subject to agricultural, municipal, and industrial wastewater effluents that likely contain different classes of endocrine-disrupting contaminants. Concern is mounting about the negative effects of environmental estrogens on fish reproduction; however, in environmental mixtures, the affects from estrogenic compounds may be suppressed by aryl hydrocarbon receptor (AhR) ligands. Indeed, reductions in 17beta-estradiol-induced (0.01 and 1 mg/kg) vitellogenin (VTG) levels were observed in white sturgeon coinjected with beta-naphthoflavone (BNF; 50 mg/kg), a model for contaminants that activate the AhR. Variation in the time of injection was used to attempt to correlate VTG inhibition to ethoxyresorufin-O-deethylase activity. No evidence was found to suggest that the inhibition of VTG is a direct result of enhanced estrogen metabolism by BNF-induced enzymes. Results of the present study are relevant for monitoring programs that measure VTG, because these results show that AhR-active environmental contaminants can repress VTG synthesis, which commonly is used as an indicator of estrogen-mimicking contaminants. Furthermore, suppression of natural estrogen signaling by AhR agonists may have significant effects on fish reproduction.
Palumbo
Amanda J
AJ
Department of Environmental Toxicology, University of California, One Shields Avenue, Davis, California 95616, USA. ajpalumbo@ucdavis.edu
Denison
Michael S
MS
Doroshov
Serge I
SI
Tjeerdema
Ronald S
RS
D000818:Animals; D019363:Cytochrome P-450 CYP1A1 / Q000378:metabolism; D015536:Down-Regulation; D004958:Estradiol / Q000494:pharmacology*; D005399:Fishes / Q000378:metabolism*; D008862:Microsomes, Liver / Q000378:metabolism; D010636:Phenols / Q000494:pharmacology*; D018336:Receptors, Aryl Hydrocarbon / Q000819:agonists*; D014819:Vitellogenins / Q000096:biosynthesis*
D010636:Phenols; D018336:Receptors, Aryl Hydrocarbon; D014819:Vitellogenins; D004958:Estradiol; C025256:nonylphenol; D019363:Cytochrome P-450 CYP1A1