NF-kappaB and Snail1a coordinate the cell cycle with gastrulation.
The cell cycle needs to strictly coordinate with developmental processes to ensure correct generation of the body plan and different tissues. However, the molecular mechanism underlying the coordination remains largely unknown. In this study, we investigate how the cell cycle coordinates gastrulation cell movements in zebrafish. We present a system to modulate the cell cycle in early zebrafish embryos by manipulating the geminin-Cdt1 balance. Alterations of the cell cycle change the apoptotic level during gastrulation, which correlates with the nuclear level of antiapoptotic nuclear factor kappaB (NF-kappaB). NF-kappaB associates with the Snail1a promoter region on the chromatin and directly activates Snail1a, an important factor controlling cell delamination, which is the initial step of mesendodermal cell movements during gastrulation. In effect, the cell cycle coordinates the delamination of mesendodermal cells through the transcription of Snail1a. Our results suggest a molecular mechanism by which NF-kappaB and Snail1a coordinate the cell cycle through gastrulation.
Liu
Xiaolin
X
Key Laboratory of Aquatic Organism Reproduction and Development, Ministry of Education, Key Laboratory of Aquatic Science of Chongqing, School of Life Science, Southwest University, Beibei, Chongqing, China.
Huang
Sizhou
S
Ma
Jun
J
Li
Chun
C
Zhang
Yaoguang
Y
Luo
Lingfei
L
D000818:Animals; D017209:Apoptosis; D036703:Blastula / Q000378:metabolism*; D002453:Cell Cycle / Q000235:genetics; D018797:Cell Cycle Proteins / Q000378:metabolism; D002465:Cell Movement; D002467:Cell Nucleus / Q000378:metabolism; D003593:Cytoplasm / Q000378:metabolism; D005775:Gastrula / Q000378:metabolism*; D054262:Gastrulation / Q000235:genetics; D018507:Gene Expression Regulation, Developmental; D009420:Nervous System / Q000196:embryology / Q000378:metabolism; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000378:metabolism; D015398:Signal Transduction / Q000235:genetics; D000071250:Snail Family Transcription Factors; D051996:Transcription Factor RelA / Q000235:genetics / Q000378:metabolism*; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D015533:Transcriptional Activation; D015027:Zebrafish; D029961:Zebrafish Proteins / Q000235:genetics / Q000378:metabolism*
D018797:Cell Cycle Proteins; D012333:RNA, Messenger; D000071250:Snail Family Transcription Factors; D051996:Transcription Factor RelA; D014157:Transcription Factors; D029961:Zebrafish Proteins; C514487:snai1a protein, zebrafish