title: Anhydrobiosis quotient: a novel approach to evaluate stability in desiccated bacterial cells.
abstract: OBJECTIVE
  The major objective of this study was the development of a methodology to quantify the anhydrobiotic ability of bacteria and its application to evaluate the stability of desiccated bacterial cells using the biocontrol agent Tsukamurella paurometabola C-924 as a model of anhydrobiote.
  
  
  RESULTS
  Tsukamurella paurometabola C-924 was desiccated by spray-drying. Samples of desiccated cells were stored at several temperatures and viability and residual moisture were measured at different intervals of time. The term anhydrobiosis quotient (epsilon) was defined, and a scale of anhydrobiotic ability for classifying micro-organisms in terms of tolerance to desiccation was established (1 < or = epsilon < or = 15). The anhydrobiosis quotient was used to evaluate the stability of the anhydrobiotic cells. As a main result, changes in the anhydrobiosis quotient at several temperatures were fitted using a reparameterized Weibull model, which was found to be robust for the prediction of the stability at 4 degrees C.
  
  
  CONCLUSIONS
  A novel methodology was developed to evaluate the desiccated state in bacteria. The anhydrobiosis quotient allows the quantitative estimation of the anhydrobiotic ability, and the mathematical model developed allows the prediction of the desiccated state of bacterial populations.
  
  
  CONCLUSIONS
  The new methodology could be applied in studying the anhydrobiosis state of bacterial populations as a predictive tool for industrial and environmental microbiology.
authors.affiliation: Center for Genetic Engineering and Biotechnology, Camagüey, Cuba. armando.hernandez@cigb.edu.cu
authors.forename: A
authors.initials: A
authors.lastname: Hernández
authors.forename: J
authors.initials: J
authors.lastname: Zamora
authors.forename: N
authors.initials: N
authors.lastname: González
authors.forename: E
authors.initials: E
authors.lastname: Salazar
authors.forename: M D C
authors.initials: MD
authors.lastname: Sánchez
mesh_terms: D000192:Actinomycetales / Q000254:growth & development* / Q000502:physiology; D015169:Colony Count, Microbial; D003890:Desiccation / Q000379:methods*; D050296:Microbial Viability; D010572:Pest Control, Biological / Q000379:methods*; D013696:Temperature