In vitro synergistic effect of gentamicin with the anti-inflammatory agent diclofenac against Listeria monocytogenes.
OBJECTIVE
A total of nine Listeria monocytogenes strains (seven serotypes) were studied to ascertain whether the non-steroidal anti-inflammatory drug diclofenac (Dc) used in combination with the conventional antilisterial antibiotic gentamicin (Gm) or ampicillin (Am) synergistically augments the efficacy of the antibiotic in vitro.


RESULTS
The effect of combination was evaluated by the checkerboard method to obtain a fractional inhibitory concentration (FIC) index followed by kill curves. Dc was synergistic with Gm (FIC 0.37) and there was indifference with Am (FIC 1) against L. monocytogenes ATCC 51774. The magnitude of the differences between killing by a single agent and the combination observed at 24 h was significant (P < 0.05) for Dc plus Gm but not Dc plus Am.


CONCLUSIONS
Thus, the ability of extended antibiotic therapy may be improved with the help of this synergistic drug pair in listeriosis.


CONCLUSIONS
Such findings may indicate parallel administration of anti-inflammatory and anti listeriosis drugs.
Dutta
N K
NK
College of Veterinary Medicine and KRF Zoonotic Disease Priority Research Institute, Seoul National University, Korea.
Mazumdar
K
K
Park
J-H
JH
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D004008:Diclofenac / Q000494:pharmacology*; D004357:Drug Synergism; D004359:Drug Therapy, Combination; D005839:Gentamicins / Q000494:pharmacology*; D006801:Humans; D008089:Listeria monocytogenes / Q000187:drug effects*; D008088:Listeriosis / Q000188:drug therapy / Q000382:microbiology
D000900:Anti-Bacterial Agents; D000893:Anti-Inflammatory Agents; D005839:Gentamicins; D004008:Diclofenac