title: Effect of cell density on adipogenic differentiation of mesenchymal stem cells.
abstract: The effect of cell density on the adipogenic differentiation of human bone marrow-derived mesenchymal stem cells (MSCs) was investigated by using a patterning technique to induce the formation of a cell density gradient on a micropatterned surface. The adipogenic differentiation of MSCs at a density gradient from 5 x 10(3) to 3 x 10(4) cells/cm2 was examined. Lipid vacuoles were observed at all cell densities after 1-3 weeks of culture in adipogenic differentiation medium although the lipid vacuoles were scarce at the low cell density and abundant at the high cell density. Real-time RT-PCR analysis showed that adipogenesis marker genes encoding peroxisome proliferator-activated receptor gamma2 (PPARgamma2), lipoprotein lipase (LPL), and fatty acid binding protein-4 (FABP4) were detected in the MSCs cultured at all cell densities. The results suggest that there was no apparent effect of cell density on the adipogenic differentiation of human MSCs.
authors.affiliation: Graduate School of Pure and Applied Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8577, Japan.
authors.forename: Hongxu
authors.initials: H
authors.lastname: Lu
authors.forename: Likun
authors.initials: L
authors.lastname: Guo
authors.forename: Michal J
authors.initials: MJ
authors.lastname: Wozniak
authors.forename: Naoki
authors.initials: N
authors.lastname: Kawazoe
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Tateishi
authors.forename: Xingdong
authors.initials: X
authors.lastname: Zhang
authors.forename: Guoping
authors.initials: G
authors.lastname: Chen
mesh_terms: D050156:Adipogenesis / Q000235:genetics; D001854:Bone Marrow Cells / Q000502:physiology* / Q000648:ultrastructure; D002452:Cell Count; D005819:Genetic Markers; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000502:physiology* / Q000648:ultrastructure; D023822:Tissue Engineering
chemical_list: D005819:Genetic Markers