Efficient and systematic synthesis of a small glycoconjugate library having human complex type oligosaccharides.
To prepare a small library of homogeneous glycoconjugates with varying oligosaccharide structures, a combinatorial strategy was employed. The target glycopeptide was divided into two peptide segments (A and B) and both were prepared by solid phase peptide synthesis. These peptides, which can be coupled by native chemical ligation through an amide bond, were subsequently coupled to two kinds of human complex type oligosaccharides. This process systematically afforded the desired glycoconjugate library.
Murase
Takefumi
T
International Graduate School of Arts and Sciences, Yokohama City University, Yokohama, Kanagawa, Japan.
Tsuji
Takashi
T
Kajihara
Yasuhiro
Y
D002240:Carbohydrate Sequence; D006020:Glycopeptides / Q000138:chemical synthesis* / Q000737:chemistry*; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D015394:Molecular Structure; D009844:Oligosaccharides / Q000737:chemistry*
D006020:Glycopeptides; D009844:Oligosaccharides