title: Oxidative stress in non-small cell lung cancer: role of nicotinamide adenine dinucleotide phosphate oxidase and glutathione.
abstract: BACKGROUND
  Cigarette smoke is strongly associated with NSCLC, but the carcinogenesis of NSCLC is poorly understood.
  
  
  METHODS
  To discover the role of oxidative stress and anti-oxidative defense in NSCLC, we measured NADPH oxidase (NOX) activity, myeloperoxidase activity, 8-OHdG, and glutathione content from lung specimens. These came from 32 patients: 22 NSCLC patients and ten controls without cancer.
  
  
  RESULTS
  In NSCLC patients, NOX activity was significantly higher both in the malignant (p = 0.001) and non-malignant (p = 0.044) samples from NSCLC patients, than in the control specimens. Myeloperoxidase activity was lower (p = 0.001) and glutathione content (p = 0.009) higher in malignant tissue. No significant difference was observable in 8-OHdG content between patient groups.
  
  
  CONCLUSIONS
  Increase in NOX activity in the malignant tissues was independent of smoking history and myeloperoxidase activity, suggesting its independent role in NSCLC pathogenesis.
authors.affiliation: Division of General Thoracic and Esophageal Surgery, Department of Cardiothoracic Surgery, Helsinki University Central Hospital, Helsinki, Finland.
authors.forename: Ilkka K
authors.initials: IK
authors.lastname: Ilonen
authors.forename: Jari V
authors.initials: JV
authors.lastname: Räsänen
authors.forename: Eero I
authors.initials: EI
authors.lastname: Sihvo
authors.forename: Aija
authors.initials: A
authors.lastname: Knuuttila
authors.forename: Kaisa M
authors.initials: KM
authors.lastname: Salmenkivi
authors.forename: Markku O
authors.initials: MO
authors.lastname: Ahotupa
authors.forename: Vuokko L
authors.initials: VL
authors.lastname: Kinnula
authors.forename: Jarmo A
authors.initials: JA
authors.lastname: Salo
mesh_terms: D000080242:8-Hydroxy-2'-Deoxyguanosine; D000230:Adenocarcinoma / Q000201:enzymology*; D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000975:Antioxidants / Q000378:metabolism; D002289:Carcinoma, Non-Small-Cell Lung / Q000201:enzymology*; D002294:Carcinoma, Squamous Cell / Q000201:enzymology*; D016022:Case-Control Studies; D003849:Deoxyguanosine / Q000031:analogs & derivatives / Q000378:metabolism; D005260:Female; D005978:Glutathione / Q000378:metabolism*; D006801:Humans; D008175:Lung Neoplasms / Q000201:enzymology*; D008297:Male; D008875:Middle Aged; D019255:NADPH Oxidases / Q000378:metabolism*; D018384:Oxidative Stress; D009195:Peroxidase / Q000378:metabolism*; D012907:Smoking / Q000009:adverse effects; D055815:Young Adult
chemical_list: D000975:Antioxidants; D000080242:8-Hydroxy-2'-Deoxyguanosine; D009195:Peroxidase; D019255:NADPH Oxidases; D003849:Deoxyguanosine; D005978:Glutathione