[Establishment and assessment of Caco-2 cell in vitro absorption model].
OBJECTIVE
To establish and assess the Caco-2 cell in vitro absorption model.


METHODS
Caco-2 cells were cultured on the millipore filters fixed in Snapwell transport chamber. The cell morphology, transepithelial electrical resistance, mannitol efflux rate and alkaline phosphatase activities were monitored during culture.


RESULTS
After 21 days of in vitro culture, formation of tight junction was observed between the cells. The transepithelial electrical resistance reach a relatively stable value of 620-/+47 Omega.cm(2), the mannitol efflux rate was lower than 0.3%.h(-1).cm(-2), and the alkaline phosphatase activity in the apical side was significantly higher than that in the basolateral side.


CONCLUSIONS
The established Caco-2 cell model shows similar morphology to intestinal epithelial cells with formation of polarity, and can be used as an in vitro model for absorption studies.
Zha
Long-Ying
LY
Department of Nutrition and Food Hygiene, School of Public Health and Tropical Medicine, Southern Medical University, Guangzhou, China. lyzha@fimmu.com
Luo
Hai-Ji
HJ
Deng
Hong
H
Chu
Xin-Wei
XW
D018938:Caco-2 Cells; D018929:Cell Culture Techniques / Q000379:methods*; D004847:Epithelial Cells / Q000166:cytology / Q000378:metabolism; D006801:Humans; D007408:Intestinal Absorption; D007422:Intestines / Q000166:cytology*