Vaccine production in Neurospora crassa.
We have chosen to use the filamentous fungus Neurospora crassa to produce subunit vaccines. Here we describe the production and purification of Influenza hemagglutinin and neuraminidase antigens in N. crassa. The N. crassa system used by Neugenesis offers many advantages over other systems for production of recombinant protein. In contrast to mammalian cell culture, N. crassa can be grown in a rapid and economic manner, generating large amounts of recombinant protein in simple, defined medium. Vaccines, therefore, can be produced more rapidly and at lower cost than conventional cell culture or egg-based systems. This has important applications to tailoring the seasonal vaccine supply and responding to new pandemics.
Allgaier
Silke
S
Neugenesis Corporation, Burlingame, CA, USA.
Taylor
Rebecca D
RD
Brudnaya
Yuliya
Y
Jacobson
David J
DJ
Cambareri
Edward
E
Stuart
W Dorsey
WD
D000595:Amino Acid Sequence; D004591:Electrophoresis, Polyacrylamide Gel; D006386:Hemagglutination Tests; D008969:Molecular Sequence Data; D009492:Neurospora crassa / Q000235:genetics*; D014614:Vaccines, Synthetic
D014614:Vaccines, Synthetic