title: Functional characterization of mesenchymal stem cells labeled with a novel PVP-coated superparamagnetic iron oxide.
abstract: Magnetic resonance imaging of cells labeled with superparamagnetic iron oxide (SPIO) could be a valuable tool for tracking transplanted cells in living organisms. Human bone marrow-derived mesenchymal stem cells (hBMMSC) were labeled with a novel polyvinyl pyrrolidone (PVP)-coated SPIO. Prussian blue staining and electron microscopy revealed that almost all of the cells were efficiently labeled with PVP-SPIO nanoparticles. There were no signs of cytotoxicity, even at concentrations of up to 1600 microg Fe/ml of the nanoparticles, and the labeled cells were successfully visualized by in vitro cellular MRI. In addition, there was no significant alteration of the phenotype or the adipo/osteo/chondrogenic differentiation potential of the cells. This was in contrast to Feridex IV labeling that led to the inhibition of hBMMSC chondrogenesis. Following intramuscular injection in a rabbit hind limb ischemia model, the intercellular migration of the labeled cells toward the ablated site was clearly tracked through in vivo MRI. The localization of the transplanted cells observed by MRI correlated well with postmortem histological studies. These results demonstrate that the novel PVP-SPIO nanoparticles appear to be efficient MR contrast agents and may enable non-invasive in vivo tracking of stem cells in experimental and clinical settings during cell therapy.
authors.affiliation: Department of Radiology, Chung-Ang University College of Medicine, Seoul, Republic of Korea.
authors.forename: Alavala Matta
authors.initials: AM
authors.lastname: Reddy
authors.forename: Byung Kook
authors.initials: BK
authors.lastname: Kwak
authors.forename: Hyung Jin
authors.initials: HJ
authors.lastname: Shim
authors.forename: Chiyoung
authors.initials: C
authors.lastname: Ahn
authors.forename: Sun Hang
authors.initials: SH
authors.lastname: Cho
authors.forename: Byung Jin
authors.initials: BJ
authors.lastname: Kim
authors.forename: Sang Young
authors.initials: SY
authors.lastname: Jeong
authors.forename: Sung-Joo
authors.initials: SJ
authors.lastname: Hwang
authors.forename: Soon Hong
authors.initials: SH
authors.lastname: Yuk
mesh_terms: D000818:Animals; D015415:Biomarkers; D016923:Cell Death; D002454:Cell Differentiation; D002470:Cell Survival; D005290:Ferric Compounds / Q000378:metabolism*; D006801:Humans; D008279:Magnetic Resonance Imaging; D008280:Magnetics; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism* / Q000648:ultrastructure; D019047:Phantoms, Imaging; D010641:Phenotype; D011205:Povidone / Q000378:metabolism*; D011817:Rabbits; D013194:Staining and Labeling
chemical_list: D015415:Biomarkers; D005290:Ferric Compounds; C000499:ferric oxide; D011205:Povidone