Array-based genomics in glioma research.
Over the years, several relevant biomarkers with a potential clinical interest have been identified in gliomas using various techniques, such as karyotype, microsatellite analysis, fluorescent in situ hybridization and chromosome comparative genomic hybridization. Despite their pivotal contribution to our understanding of gliomas biology, clinical application of these approaches has been limited by technological and clinical complexities. In contrast, genomic arrays (array-based comparative genomic hybridization and single nucleotide polymorphisms array) have emerged as promising technologies for clinical use in the setting of gliomas. Indeed, their feasibility and reliability have been rigorously assessed in gliomas and are discussed in this review. The well-known genomic biomarkers in gliomas are in fact readily and reliably identified using genomic arrays. Moreover, it detects a multitude of new cryptic genomic markers, with potential biological and/or clinical significances. The main studies dedicated to genomic characterization of gliomas using genomic arrays are reviewed here. Interestingly, several recurrent genomic signatures have been reported by different teams, suggesting the validity of these genomic patterns. In light of this, genomic arrays are relatively simple and cost-effective techniques whose implementation in molecular diagnostic laboratories should be encouraged as a valuable clinical tool for management of glioma patients.
Idbaih
Ahmed
A
INSERM, Unité 711, Paris, France. ahmed.idbaih@gmail.com
Crinière
Emmanuelle
E
Ligon
Keith L
KL
Delattre
Olivier
O
Delattre
Jean-Yves
JY
D000818:Animals; D001253:Astrocytes / Q000473:pathology; D001254:Astrocytoma / Q000473:pathology; D001932:Brain Neoplasms / Q000235:genetics* / Q000473:pathology*; D023281:Genomics; D005909:Glioblastoma / Q000473:pathology; D005910:Glioma / Q000235:genetics* / Q000473:pathology*; D006801:Humans; D009837:Oligodendroglioma / Q000473:pathology; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*