title: Comparison of screening methods for antibiotics in beef kidney juice and serum.
abstract: Rapid screening tests can be used as part of an efficient program designed to monitor veterinary drug residues in cattle. In this work, three rapid tests designed to screen samples for the presence of antibiotic residues, the Fast Antimicrobial Screen Test (FAST), Premi and Kidney Inhibition Swab (KIS) tests, were compared using beef kidney juice and serum samples. In order to provide a realistic assessment, potentially incurred samples of beef kidney juice and serum were obtained from 235 carcasses which had been retained by inspectors in a processing plant for further testing. In addition, liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis was conducted on these samples to identify what antibiotics were present, if any, and their levels. The comparison of the three rapid screening test results with those from LC-MS/MS analysis allowed for a more complete comparison of the relative sensitivity of these analytical methods, as well as valuable information on false positive and negative response rates.
authors.affiliation: USDA-ARS, Eastern Regional Research Center, 600 E. Mermaid Lane, Wyndmoor, PA 19038 USA.
authors.forename: Marilyn J
authors.initials: MJ
authors.lastname: Schneider
authors.forename: Katerina
authors.initials: K
authors.lastname: Mastovska
authors.forename: Steven J
authors.initials: SJ
authors.lastname: Lehotay
authors.forename: Alan R
authors.initials: AR
authors.lastname: Lightfield
authors.forename: Brian
authors.initials: B
authors.lastname: Kinsella
authors.forename: Craig E
authors.initials: CE
authors.lastname: Shultz
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000032:analysis* / Q000097:blood; D002417:Cattle; D002853:Chromatography, Liquid / Q000379:methods*; D004350:Drug Residues / Q000032:analysis*; D005188:False Negative Reactions; D005189:False Positive Reactions; D007668:Kidney / Q000737:chemistry*; D008826:Microbial Sensitivity Tests / Q000379:methods*; D011933:Reagent Kits, Diagnostic; D012680:Sensitivity and Specificity; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D000900:Anti-Bacterial Agents; D011933:Reagent Kits, Diagnostic