Utilization of an enantioselective surface plasmon resonance electrode for the selection of the best C(70) fullerene as chiral selector for the enantioanalysis of L-cysteine.
Two novel enantioselective surface plasmon resonance (SPR) sensors based on self-assembled monolayer of (5-6)-fullerene-C(70) and diethyl (1,2-methanofullerene C(70))-71-71-dicarboxylate as chiral selectors are proposed. Binding assay, apparent affinity constant, and apparent dissociation binding constant have been used to analyze and study the enantioselectivity of C(70) fullerene-octadecanethiol film for L-cysteine which was chosen as model analyte. The apparent affinity constant for the complex formed by L-cysteine with (5-6)-fullerene-C(70) and diethyl (1,2-methanofullerene C(70))-71-71-dicarboxylate films were 1 x 10(10) L/mol, and 6.7 x 10(8) L/mol, respectively. Accordingly, the chiral selector of choice will be (5-6)-fullerene-C(70). No binding was recorded between any of the tested C(70) fullerenes and D-cysteine, this proving the enantioselectivity of the proposed SPR sensors.
van Staden
Raluca-Ioana Stefan
RI
Laboratory of Electrochemistry Bucharest, National Institute of Research for Electrochemistry and Condensed Matter, Bucharest, Romania. iustinavanstaden@yahoo.com
Holo
Luxolo
L
D003545:Cysteine / Q000032:analysis* / Q000737:chemistry*; D004566:Electrodes; D037741:Fullerenes / Q000737:chemistry*; D012680:Sensitivity and Specificity; D013237:Stereoisomerism; D020349:Surface Plasmon Resonance / Q000295:instrumentation* / Q000379:methods*; D013499:Surface Properties
D037741:Fullerenes; C069836:fullerene C70; D003545:Cysteine