Glycerol utilization gene cluster in Streptomyces clavuligerus.
The Streptomyces clavuligerus ATCC 27064 glycerol cluster gylR-glpF1K1D1 is induced by glycerol but is not affected by glucose. S. clavuligerus growth and clavulanic acid production are stimulated by glycerol, but this does not occur in a glpK1-deleted mutant. Amplification of glpK1D1 results in transformants yielding larger amounts of clavulanic acid in the wild-type strain and in overproducer S. clavuligerus Gap15-7-30 or S. clavuligerus Delta relA strains.
Baños
Sonia
S
Area de Microbiología, Facultad de Ciencias Biológicas y Ambientales, Universidad de León, 24071 León, Spain.
Pérez-Redondo
Rosario
R
Koekman
Bert
B
Liras
Paloma
P
D001483:Base Sequence; D019818:Clavulanic Acid / Q000096:biosynthesis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D017353:Gene Deletion; D018628:Gene Dosage; D020869:Gene Expression Profiling; D023061:Gene Order; D005798:Genes, Bacterial; D005947:Glucose / Q000378:metabolism; D005990:Glycerol / Q000378:metabolism*; D053858:Metabolic Networks and Pathways / Q000235:genetics*; D008969:Molecular Sequence Data; D005810:Multigene Family; D017422:Sequence Analysis, DNA; D013302:Streptomyces / Q000235:genetics* / Q000254:growth & development
D004269:DNA, Bacterial; D019818:Clavulanic Acid; D005947:Glucose; D005990:Glycerol