Inhibitory effect of leptin on growth hormone secretion of GH3 cells: involvement of cell proliferation, apoptosis and intracellular free Ca2+.
Recent studies have identified leptin and leptin receptors in the pituitary of different species, which suggest that there may be endocrine and paracrine regulatory roles between leptin-producing cells and cells with leptin receptor in pituitary, including growth and secretion of GH cells. The aim of this study was to investigate the effects of leptin on growth hormone (GH) secretion of GH3 cell. GH3 cells were cultured and treated with leptin. Cell proliferation was evaluated by MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide) assay, distribution of cell cycle and rate of apoptosis determined by flow cytometry and fluorescence microscopy, and intracellular free Ca(2+) levels ([Ca(2+)](i)) of single GH3 cells measured by a laser scanning confocal microscope. Leptin (10(-9)-10(-7)mol/L) at 1day or longer of treatment inhibited the basal growth hormone secretion of GH3 cells (P<0.05), but had no significant effect on short-term treatment. Leptin inhibited cell proliferation, reduced the proportion of cells in DNA synthesis period (S phase) to inhibit DNA synthesis of GH3 cells, and accelerated cell apoptosis of GH3 cells. Furthermore, the level of [Ca(2+)](i) of single GH3 cell was found to decrease immediately upon the addition of leptin (10(-8)mol/L). Leptin inhibits the basal GH secretion of GH3 cells, which may be due to the inhibition of proliferation, DNA synthesis and advanced apoptosis of GH3 cell. The inhibition of leptin on GH synthesis and secretion may be related to intracellular free Ca(2+) level.
Liu
Yali
Y
Department of Physiology, State Key Discipline of Cell Biology, The Fourth Military Medical University, No. 17 West Changle Road, Shaanxi, Xi'an 710032, China.
Zhong
Yanqing
Y
Pei
Jianming
J
Zhu
Yunlong
Y
Hu
Yuzhen
Y
Chi
Sumin
S
Kaye
Alan D
AD
D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D002118:Calcium / Q000378:metabolism*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D013006:Growth Hormone / Q000378:metabolism*; D020738:Leptin / Q000378:metabolism / Q000494:pharmacology; D010902:Pituitary Gland / Q000166:cytology*; D051381:Rats
D020738:Leptin; D013006:Growth Hormone; D002118:Calcium