Detection of myeloperoxidase activity in primary leukemic cells by an enhanced chemiluminescent assay for differentiation between acute lymphoblastic and non-lymphoblastic leukemia.
BACKGROUND
Myeloperoxidase (MPO) plays a crucial role in the differentiation of acute lymphoblastic leukemia (ALL) and acute non-lymphoblastic leukemia (ANLL). In this report, we proposed the application of the enhanced chemiluminescent (ECL) technique to the determination of MPO activity in blasts of acute leukemia (AL).


METHODS
Bone-marrow samples were obtained from 23 patients with AL (ALL, 5 cases; ANLL, 13 cases; AUL, 1 cases; mixed-lineage AL, 4 cases). Cells were incubated with a standard reaction mixture and chemiluminescence was measured. The mean peak light emission (PLE) was assessed.


RESULTS
When the cut-off point of PLE was settled at 2483, which was set for the discrimination between ANLL and ALL (mean + 3 x SD of ALL samples, n=5), all cases of ALL were MPO-negative, and ten of the thirteen ANLL patients were MPO-positive, which was concordant with cytochemical staining. In addition, this technique was able to demonstrate MPO activity in 4 mixed-lineage AL cases which did not stain for MPO in cytochemistry preparations.


CONCLUSIONS
Our ECL technique is simple, inexpensive, and easier to perform compared to other procedures used to measure MPO activity in AL.
Tan
Sanqin
S
Department of Hematology, Xiangya Hospital of Central South University, Changsha, Hunan 410008, China.
Wang
Guangping
G
Peng
Minyuan
M
Zhang
Xinhua
X
Shen
Guoli
G
Jiang
Jianhui
J
Chen
Fangping
F
D000293:Adolescent; D000328:Adult; D000368:Aged; D002648:Child; D003937:Diagnosis, Differential; D005260:Female; D018922:HL-60 Cells; D006801:Humans; D007223:Infant; D015470:Leukemia, Myeloid, Acute / Q000175:diagnosis / Q000201:enzymology* / Q000473:pathology*; D008163:Luminescent Measurements / Q000379:methods*; D008297:Male; D008875:Middle Aged; D009195:Peroxidase / Q000032:analysis / Q000378:metabolism*; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000175:diagnosis / Q000201:enzymology* / Q000473:pathology*
D009195:Peroxidase