[Effect of electroacupuncture on inositol triphosphate and diacylglycerol levels in cerebral arteries of cerebral ischemia rats].
OBJECTIVE
To observe the effect of electroacupuncture (EA) on inositol triphosphate (IP3) and diacylglycerol (DAG) contents in cerebral arteries of the cerebral cortex in rats with cerebral ischemia (CI), so as to explore its effect on intracellular signal transduction pathway.


METHODS
Fifty-six Wistar rats wererandomly divided into control (n=8), CI (n=24) and EA (n=24) groups, and the later 2 groups were further separately divided into 1 h, 3 h and 6 h subgroups (CI-1 h, CI-3 h, CI-6 h, EA-CI-1 h, EA-CI-3 h and EA-CI-6 h), with 8 cases in each. CI model was made by occlusion of the middle cerebral artery. EA (15 Hz, 0.1 mA) was applied to "Shuigou" (GV 26) for 20 min. The cerebral arteries in the cerebral shallow layer were collected for assaying intracellular DAG content by thin-layer chromatography (TLC) and IP3 content by competitive protein binding assay (CPBA).


RESULTS
Compared with control group,the levels of IP3 and DAG in cerebral vascular smooth muscle were significantly increased in model 1 h, 3 h and 6 h subgroups (P<0.01). Compared with the corresponding model subgroups,the levels of IP3 and DAG were significantly decreased in EA-CI-1 h, EA-CI-3 h and EA-CI-6 h subgroups (P<0.01).


CONCLUSIONS
EA of GV 26 can effectively inhibit CI-induced increase of intracellular IP3 and DAG levels in rat cerebral arteries, which may contribute to its effect in modulating vascular motor to ameliorate cerebral microcirculation.
Sun
Dong-wei
DW
Tianjin University of Chinese Medicine,Tianjin 300193, China. dong2276@sina.com.cn
Du
Yuan-hao
YH
Shi
Lei
L
D015669:Acupuncture Points; D000818:Animals; D002545:Brain Ischemia / Q000378:metabolism* / Q000628:therapy*; D002536:Cerebral Arteries / Q000378:metabolism*; D004075:Diglycerides / Q000378:metabolism*; D015671:Electroacupuncture; D006801:Humans; D015544:Inositol 1,4,5-Trisphosphate / Q000378:metabolism*; D008297:Male; D009130:Muscle, Smooth / Q000378:metabolism; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar
D004075:Diglycerides; D015544:Inositol 1,4,5-Trisphosphate