The role of vascular adhesion molecules PECAM-1 (CD 31), VCAM-1 (CD 106), E-selectin (CD62E) and P-selectin (CD62P) in severe porcine pancreatitis.
Inflammatory cytokines have been shown to mediate organ damage by their action on vascular endothelia and leukocytes, in part by upregulating the expression of adhesion molecules, which in turn convey transmigration of leukocytes into tissue. The upregulation and activation of vascular cell adhesion molecules on the endothelial cells avail firm leukocyte adhesion to the vascular endothelium and enhance their transmigration and consecutive tissue injury. The aim of this study was to evaluate the expression of vascular adhesion molecules CD 31 (PECAM-1), CD 106 (VCAM-1), CD 62E (E-Selectin) and CD 62P (P-Selectin) in the pancreas and distant organs of pigs suffering from acute necrotizing pancreatitis (AP). AP was induced in 13 pigs by a combination of intravenous cerulein and intraductal glycodeoxycholic acid. For immunostaining of vascular adhesion molecules slides of porcine pancreas, lung, kidney and liver tissue were stained with monoclonal antibodies (Ab) against PECAM-1-1, VCAM-1 E- and P- SELECTIN. The endothelial cell expression of CD 31 (PECAM-1), CD 106 (VCAM), CD 62E (E-Selectin) and CD 62P (P-SELECTIN) in severe porcine pancreatitis is detectable and upregulation is partly significantly.
Kleinhans
Helge
H
Department of General-, Visceral- and Thoracic-Surgery, University Medical Center Hamburg-Eppendorf, Hamburg, Germany. dr.kleinhans@gmx.de
Kaifi
Jussuf T
JT
Mann
Oliver
O
Reinknecht
Felix
F
Freitag
Marc
M
Hansen
Bente
B
Schurr
Paulus G
PG
Izbicki
Jakob R
JR
Strate
Tim G
TG
D000818:Animals; D002448:Cell Adhesion / Q000502:physiology; D002465:Cell Movement / Q000502:physiology; D019040:E-Selectin / Q000502:physiology*; D042783:Endothelial Cells / Q000502:physiology; D007150:Immunohistochemistry; D018836:Inflammation Mediators / Q000502:physiology; D007668:Kidney / Q000503:physiopathology; D007962:Leukocytes / Q000502:physiology; D008099:Liver / Q000502:physiology; D008168:Lung / Q000503:physiopathology; D019007:P-Selectin / Q000502:physiology*; D010179:Pancreas / Q000503:physiopathology; D019283:Pancreatitis, Acute Necrotizing / Q000209:etiology* / Q000503:physiopathology*; D019408:Platelet Endothelial Cell Adhesion Molecule-1 / Q000502:physiology*; D013552:Swine; D015854:Up-Regulation; D019010:Vascular Cell Adhesion Molecule-1 / Q000502:physiology*
D019040:E-Selectin; D018836:Inflammation Mediators; D019007:P-Selectin; D019408:Platelet Endothelial Cell Adhesion Molecule-1; D019010:Vascular Cell Adhesion Molecule-1