title: Profiling CD antigens on leukaemias with an antibody microarray.
abstract: Cluster of differentiation (CD) antigens are defined when a surface molecule found on some members of a standard panel of human cells reacts with at least one novel antibody, and there is good accompanying molecular data. Monoclonal antibodies to surface CD antigens on leukocytes have been used for flow cytometry, and more recently to construct microarrays that capture live cells. These DotScan microarrays enable the rapid and highly parallel characterization of repertoires of CD antigens whose expression patterns may be correlated with discrete leukaemia subtypes, or used to define biomarker 'signatures' for non-hematological diseases. DotScan with fluorescence multiplexing enables profiling of CD antigens for minor subsets of cells, such as colorectal cancer cells and tumour-infiltrating lymphocytes from a surgical sample.
authors.affiliation: School of Molecular and Microbial Biosciences, University of Sydney, Sydney, NSW 2006, Australia.
authors.forename: Nicole
authors.initials: N
authors.lastname: Barber
authors.forename: Swetlana
authors.initials: S
authors.lastname: Gez
authors.forename: Larissa
authors.initials: L
authors.lastname: Belov
authors.forename: Stephen P
authors.initials: SP
authors.lastname: Mulligan
authors.forename: Adrian
authors.initials: A
authors.lastname: Woolfson
authors.forename: Richard I
authors.initials: RI
authors.lastname: Christopherson
mesh_terms: D000911:Antibodies, Monoclonal / Q000276:immunology*; D015703:Antigens, CD / Q000276:immunology*; D006801:Humans; D007938:Leukemia / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D015703:Antigens, CD