title: Identification of tumor-associated antigens by using SEREX in hepatocellular carcinoma.
abstract: OBJECTIVE
  To identify biomarkers for diagnosis and prognosis of hepatocellular carcinoma (HCC).
  
  
  METHODS
  Screening the HCC cDNA library with HCC patients sera. Isolated proteins were used as antigens to detect antibodies from patients with HCC and control sera.
  
  
  RESULTS
  Eighty-one positive clones were identified. The frequencies of autoantibody against five HCC-associated antigens were higher in HCC than that in chronic hepatitis and normal human sera. The sensitivity and specificity of KRT23, AHSG and FTL antigens combination tests up to 98.2% in joint test and 90.0% in series test separately.
  
  
  CONCLUSIONS
  HCC associate antigens identified from this study supply candidate markers of diagnosis, combined detection and immunotherapy of HCC.
authors.affiliation: Department of Epidemiology, Zhengzhou University, China.
authors.forename: Kaijuan
authors.initials: K
authors.lastname: Wang
authors.forename: Xueqin
authors.initials: X
authors.lastname: Xu
authors.forename: Yifei
authors.initials: Y
authors.lastname: Nie
authors.forename: Liping
authors.initials: L
authors.lastname: Dai
authors.forename: Peng
authors.initials: P
authors.lastname: Wang
authors.forename: Jianying
authors.initials: J
authors.lastname: Zhang
mesh_terms: D000595:Amino Acid Sequence; D000951:Antigens, Neoplasm / Q000235:genetics*; D001323:Autoantibodies / Q000097:blood; D014408:Biomarkers, Tumor / Q000032:analysis*; D006528:Carcinoma, Hepatocellular / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D015723:Gene Library; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D008969:Molecular Sequence Data; D012680:Sensitivity and Specificity
chemical_list: D000951:Antigens, Neoplasm; D001323:Autoantibodies; D014408:Biomarkers, Tumor