Location of antigenic sites recognized by monoclonal antibodies in the influenza A virus nucleoprotein molecule.
The locations of amino acid positions relevant to antigenic variation in the nucleoprotein (NP) of influenza virus are not conclusively known. We analysed the antigenic structure of influenza A virus NP by introducing site-specific mutations at amino acid positions presumed to be relevant for the differentiation of strain differences by anti-NP monoclonal antibodies. Mutant proteins were expressed in a prokaryotic system and analysed by performing ELISA with monoclonal antibodies. Four amino acid residues were found to determine four different antibody-binding sites. When mapped in a 3D X-ray model of NP, the four antigenically relevant amino acid positions were found to be located in separate physical sites of the NP molecule.
Varich
Natalia L
NL
D. I. Ivanovsky Institute of Virology, Gamaleya Str. 16, 123098 Moscow, Russia.
Kochergin-Nikitsky
Konstantin S
KS
D. I. Ivanovsky Institute of Virology, Gamaleya Str. 16, 123098 Moscow, Russia.
Usachev
Evgeny V
EV
D. I. Ivanovsky Institute of Virology, Gamaleya Str. 16, 123098 Moscow, Russia.
Usacheva
Olga V
OV
D. I. Ivanovsky Institute of Virology, Gamaleya Str. 16, 123098 Moscow, Russia.
Prilipov
Alexei G
AG
D. I. Ivanovsky Institute of Virology, Gamaleya Str. 16, 123098 Moscow, Russia.
Webster
Robert G
RG
Department of Pathology, University of Tennessee, Memphis, TN 38105, USA.|Division of Virology, Department of Infectious Diseases, St Jude Children's Research Hospital, 262 Danny Thomas Place, Memphis, TN 38105 3678, USA.
Kaverin
Nikolai V
NV
D. I. Ivanovsky Institute of Virology, Gamaleya Str. 16, 123098 Moscow, Russia.
D019943:Amino Acid Substitution / Q000235:genetics; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000914:Antibodies, Viral / Q000276:immunology*; D001666:Binding Sites, Antibody; D004797:Enzyme-Linked Immunosorbent Assay; D018985:Epitopes, B-Lymphocyte / Q000276:immunology*; D008958:Models, Molecular; D016297:Mutagenesis, Site-Directed; D019590:Nucleocapsid Proteins; D017434:Protein Structure, Tertiary; D016601:RNA-Binding Proteins / Q000276:immunology*; D014758:Viral Core Proteins / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D018985:Epitopes, B-Lymphocyte; C048842:NP protein, Influenza A virus; D019590:Nucleocapsid Proteins; D016601:RNA-Binding Proteins; D014758:Viral Core Proteins