title: Antinociceptive properties of the hydroalcoholic extract, fractions and compounds obtained from the aerial parts of Baccharis illinita DC in mice.
abstract: The present study assessed the possible antinociceptive action of the hydroalcoholic extract, fractions and pure compounds obtained from the aerial parts of Baccharis illinita DC (Asteraceae) in behavioural models of chemical nociception in mice. The hydroalcoholic extract and fractions (hexane and aqueous but not EtOAc fraction) obtained from B. illinita (30-1000 mg/kg orally) produced a dose-related inhibition of the acetic acid-induced nociceptive response. However, the hexane fraction was more potent than the hydroalcoholic extract and the aqueous fraction. The hexane fraction derivatives baurenol, alpha-spinasterol and oleanolic acid (0.00001-10 mg/kg intraperitoneally) also caused potent inhibition of acetic acid-induced pain. The hexane fraction (300-1000 mg/kg orally) produced inhibition of both phases of formalin-induced pain. Moreover, the hexane fraction (30-600 mg/kg orally) also caused a dose-dependent inhibition of glutamate-induced pain. Nevertheless, the hexane fraction only at the dose of 300 mg/kg orally, produced partial inhibition of the paw oedema caused by carrageenan. Furthermore, the hexane fraction (300 mg/kg orally) caused inhibition of the nociceptive response induced by intrathecal injection of N-methyl-d-aspartic acid, alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid, tumour necrosis factor-alpha and interleukin-1beta. In contrast, the hexane fraction did not affect the biting response induced by the metabotropic or ionotropic glutamatergic receptor agonist (+/-)-1-aminocyclopentane-trans-1,3-dicarboxylic acid and kainate, respectively. In addition, the antinociception caused by the hexane fraction (300 mg/kg orally) in the acetic acid test was not affected by intraperitoneal treatment of mice with naloxone (a non-selective opioid receptor antagonist). The precise mechanism responsible for the antinociceptive effect of the hexane fraction remains unclear, but appears to be partly associated with an inhibition of glutamatergic transmission and an inhibition of pathways dependent on pro-inflammatory cytokines. Finally, baurenol, alpha-spinasterol and oleanolic acid have an important role in the antinociceptive effects of the hexane fraction. Moreover, the antinociceptive action demonstrated in the present study supports the ethnomedical uses of this plant.
authors.affiliation: Department of Pharmacology, Sector of Biological Sciences, Federal University of Paraná, Curitiba, PR, Brazil.
authors.forename: Cristina Setim
authors.initials: CS
authors.lastname: Freitas
authors.forename: Cristiane Hatsuko
authors.initials: CH
authors.lastname: Baggio
authors.forename: Ana Cristina
authors.initials: AC
authors.lastname: Dos Santos
authors.forename: Bárbara
authors.initials: B
authors.lastname: Mayer
authors.forename: André
authors.initials: A
authors.lastname: Twardowschy
authors.forename: Ana Paula
authors.initials: AP
authors.lastname: Luiz
authors.forename: Rodrigo
authors.initials: R
authors.lastname: Marcon
authors.forename: Cristian
authors.initials: C
authors.lastname: Soldi
authors.forename: Moacir G
authors.initials: MG
authors.lastname: Pizzolatti
authors.forename: Elide Pereira
authors.initials: EP
authors.lastname: Dos Santos
authors.forename: Maria Consuelo Andrade
authors.initials: MC
authors.lastname: Marques
authors.forename: Adair R S
authors.initials: AR
authors.lastname: Santos
mesh_terms: D000700:Analgesics / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology*; D000818:Animals; D031175:Baccharis / Q000737:chemistry*; D016207:Cytokines / Q000187:drug effects / Q000378:metabolism; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D005260:Female; D051379:Mice; D009828:Oleanolic Acid / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology; D010146:Pain / Q000188:drug therapy*; D010147:Pain Measurement; D035261:Plant Components, Aerial; D010936:Plant Extracts / Q000008:administration & dosage / Q000494:pharmacology*; D017470:Receptors, Glutamate; D012997:Solvents / Q000737:chemistry; D013265:Stigmasterol / Q000008:administration & dosage / Q000031:analogs & derivatives / Q000302:isolation & purification / Q000494:pharmacology; D014315:Triterpenes / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology
chemical_list: D000700:Analgesics; D016207:Cytokines; D010936:Plant Extracts; D017470:Receptors, Glutamate; D012997:Solvents; D014315:Triterpenes; C541218:baurenol; C031312:spinasterol; D009828:Oleanolic Acid; D013265:Stigmasterol