title: Difference in killing activity of caspofungin and paradoxical growth between Candida albicans and C. krusei clinical isolates in different media.
abstract: Minimum fungicidal concentration (mFC) of caspofungin was determined against 16 Candida albicans and 16 C. krusei in Rpmi-1640 and antibiotic medium 3 (Am3). time-kill tests were performed on six C. albicans and four C. krusei strains at 0.06-16 mg/l caspofungin. mFC ranges after 48 h were 0.5-1 and 1-2 mg/l for C. albicans and C. krusei, respectively; one C. albicans and the C. krusei reference strain showed paradoxical growth (pG) in Rpmi-1640, respectively. in killing experiments, after 48 h caspofugin was fungicidal against two and four C. albicans in Rpmi-1640 (at 16 mg/l) and in Am3 (at >0.5 mg/l), respectively; pG was noted in three and two cases, respectively. Caspofungin at >2 and 0.5 mg/l was fungicidal against all tested C. krusei strains even after 24 h in Rpmi-1640 and Am3, respectively. Killing activity of caspofungin against C. albicans and C. krusei could be exactly measured only by killing curves.
authors.affiliation: Medical and Health Science Center, University of Debrecen, Hungary.
authors.forename: I
authors.initials: I
authors.lastname: Varga
authors.forename: G
authors.initials: G
authors.lastname: Sóczó
authors.forename: G
authors.initials: G
authors.lastname: Kardos
authors.forename: A
authors.initials: A
authors.lastname: Kemény-Beke
authors.forename: B
authors.initials: B
authors.lastname: Kelentey
authors.forename: I
authors.initials: I
authors.lastname: Márton
authors.forename: L
authors.initials: L
authors.lastname: Majoros
mesh_terms: D000935:Antifungal Agents / Q000494:pharmacology*; D002175:Candida / Q000187:drug effects / Q000254:growth & development; D002176:Candida albicans / Q000187:drug effects* / Q000254:growth & development*; D000077336:Caspofungin; D018929:Cell Culture Techniques / Q000379:methods*; D003470:Culture Media; D054714:Echinocandins / Q000494:pharmacology*; D055666:Lipopeptides; D008826:Microbial Sensitivity Tests
chemical_list: D000935:Antifungal Agents; D003470:Culture Media; D054714:Echinocandins; D055666:Lipopeptides; D000077336:Caspofungin