Optimizing the performance of tin dioxide microspheres for phosphopeptide enrichment.
Phosphopeptide enrichment based on metal oxide affinity chromatography is one of the most powerful tools for studying protein phosphorylation on a large scale. To complement existing metal oxide sorbents, we have recently introduced tin dioxide as a promising alternative. The preparation of SnO(2) microspheres by the nanocasting technique, using silica of different morphology as a template, offers a strategy to prepare materials that vary in their particle size and their porosity. Here, we demonstrate how such stannia materials can be successfully generated and their properties fine-tuned in order to obtain an optimized phosphopeptide enrichment material. We combined data from liquid chromatography-mass spectrometry experiments and physicochemical characterization, including nitrogen physisorption and energy-dispersive X-ray spectroscopy (EDX), to explain the influence of the various experimental parameters.
Leitner
Alexander
A
Department of Analytical Chemistry and Food Chemistry, University of Vienna, Waehringer Strasse 38, 1090 Vienna, Austria. alexander.leitner@univie.ac.at
Sturm
Martin
M
Smått
Jan-Henrik
JH
Järn
Mikael
M
Lindén
Mika
M
Mechtler
Karl
K
Lindner
Wolfgang
W
D000595:Amino Acid Sequence; D002846:Chromatography, Affinity; D002853:Chromatography, Liquid; D013058:Mass Spectrometry; D008863:Microspheres; D036103:Nanotechnology; D010316:Particle Size; D010748:Phosphopeptides / Q000032:analysis* / Q000302:isolation & purification; D010766:Phosphorylation; D016062:Porosity; D017971:Tin Compounds / Q000737:chemistry*
D010748:Phosphopeptides; D017971:Tin Compounds; C045358:stannic oxide