Soluble HLA-G in IVF/ICSI embryo culture supernatants does not always predict implantation success: a multicentre study.
Several reports have described an association between the presence of soluble human leukocyte antigen G (sHLA-G) in human embryo culture supernatants (ES) and implantation success. However, not all studies agree with these findings. To further document this debate, a multicentre blinded study was performed to investigate, on a large number of IVF ES and ICSI ES, whether sHLA-G is a useful criterion for embryo selection before transfer. A total of 1405 ES from 355 patients were collected from three assisted reproductive technique (ART) centres and evaluated for their sHLA-G content in a single laboratory, using a chemiluminescence enzyme-linked immunosorbent assay. In only one centre was a significant association between sHLA-G-positive ES and successful implantation established (P = 0.0379), whereas no such association was observed in the other centres. It was found that the percentages and concentrations of sHLA-G-positive ES varied between centres, depending on culture media and ART conditions. The percentage of sHLA-G-positive ES was significantly higher in IVF ES than ICSI ES (P < 0.001 and P < 0.01 for two centres). These data demonstrate that substantial variations of sHLA-G content in ES occur between different ART centres, highlighting the influence of several technical parameters that differ from one centre to another.
Tabiasco
Julie
J
INSERM, U563, Centre de Physiopathologie de Toulouse Purpan, Toulouse, F-31300 France.
Perrier d'Hauterive
Sophie
S
Thonon
Fabienne
F
Parinaud
Jean
J
Léandri
Roger
R
Foidart
Jean-Michel
JM
Chaouat
Gérard
G
Munaut
Carine
C
Lombroso
Raoul
R
Selva
Jacqueline
J
Bergère
Marianne
M
Hammoud
Ibrahim
I
Kozma
Noemi
N
Aguerre-Girr
Maryse
M
Swales
Anna K E
AK
Sargent
Ian L
IL
Le Bouteiller
Philippe
P
Lédée
Nathalie
N
D000328:Adult; D003470:Culture Media; D004797:Enzyme-Linked Immunosorbent Assay; D005307:Fertilization in Vitro; D006680:HLA Antigens / Q000032:analysis*; D059951:HLA-G Antigens; D015395:Histocompatibility Antigens Class I / Q000032:analysis*; D006801:Humans; D049449:Luminescence; D020554:Sperm Injections, Intracytoplasmic
D003470:Culture Media; D006680:HLA Antigens; D059951:HLA-G Antigens; D015395:Histocompatibility Antigens Class I