title: Naïve human umbilical cord matrix derived stem cells significantly attenuate growth of human breast cancer cells in vitro and in vivo.
abstract: The effect of un-engineered (naïve) human umbilical cord matrix stem cells (hUCMSC) on the metastatic growth of MDA 231 xenografts in SCID mouse lung was examined. Three weekly IV injections of 5x10(5) hUCMSC significantly attenuated MDA 231 tumor growth as compared to the saline-injected control. IV injected hUCMSC were detected only within tumors or in close proximity to the tumors. This in vivo result was corroborated by multiple in vitro studies such as colony assay in soft agar and [(3)H]-thymidine uptake. These results suggest that naïve hUCMSC may be a useful tool for cancer cytotherapy.
authors.affiliation: Department of Anatomy and Physiology, Kansas State University College of Veterinary Medicine, Manhattan, KS 66506, United States.
authors.forename: Rie
authors.initials: R
authors.lastname: Ayuzawa
authors.forename: Chiyo
authors.initials: C
authors.lastname: Doi
authors.forename: Raja Shekar
authors.initials: RS
authors.lastname: Rachakatla
authors.forename: Marla M
authors.initials: MM
authors.lastname: Pyle
authors.forename: Dharmendra Kumar
authors.initials: DK
authors.lastname: Maurya
authors.forename: Deryl
authors.initials: D
authors.lastname: Troyer
authors.forename: Masaaki
authors.initials: M
authors.lastname: Tamura
mesh_terms: D000818:Animals; D001943:Breast Neoplasms / Q000378:metabolism* / Q000628:therapy*; D002453:Cell Cycle; D045744:Cell Line, Tumor; D018920:Coculture Techniques; D005260:Female; D006801:Humans; D020935:MAP Kinase Signaling System; D051379:Mice; D016513:Mice, SCID; D009362:Neoplasm Metastasis; D009368:Neoplasm Transplantation; D013234:Stem Cells / Q000166:cytology* / Q000378:metabolism; D014470:Umbilical Cord / Q000166:cytology* / Q000378:metabolism; D014471:Umbilical Veins