title: The comparison of the effects of different doses of levobupivacaine infiltration on wound healing.
abstract: INTRODUCTION-AIM: The easiest method in postoperative analgesia is the infiltration of the wound with local anesthetic drugs. Although many local anesthetic drugs have been used for this type of infiltration, studies on levobupivacaine are rare. The aim of this study was to investigate the effects of different concentrations of levobupivacaine infiltration on wound healing.
  
  
  METHODS
  Forty female Wistar-Albino rats (280-300 g) were included in the study, which were randomly separated into four groups. Rats were infiltrated with 1.25 mg/mL levobupivacaine in group L(1.25) (n = 10), with 2.50 mg/mL levobupivacaine in group L(2.5) (n = 10), with 3.75 mg/mL levobupivacaine in group L(3.75) (n = 10), and with normal saline in control group (n = 10). Breaking-strength measurements, levels of hydroxyproline, and fibrotic index were evaluated in the tissue samples taken from the rats.
  
  
  RESULTS
  When the breaking-strength measurements were evaluated, we have found a significant difference between the control and the study groups (p < 0.05). In the intergroup comparison the difference between groups L(1.25) and L(3.75) was statistically significant (p < 0.05). In all of the levobupivacaine groups the levels of hydroxyproline were higher compared to the control group. Also significant differences were observed between groups L(1.25) and L(2.5) and groups L(1.25) and L(3.75) (p < 0.05). The levels of tissue fibrotic index were higher in all of the levobupivacaine groups compared to the control group (p < 0.05) and also a difference was observed between groups L(1.25) and L(3.75) in terms of tissue fibrotic index (p < 0.05).
  
  
  CONCLUSIONS
  We have concluded that levobupivacaine used in clinical doses have a significant effect on the fastening of wound healing and this effect increases with an increase in the concentration of the levobupivacaine. We believe that levobupivacaine will be more widely preferred in the near future in the postoperative analgesia.
authors.affiliation: Gulhane Military Medical Academy, Department of Anesthesiology, Haydarpasa Training Hospital, Istanbul, Turkey.
authors.forename: Kamer
authors.initials: K
authors.lastname: Dere
authors.forename: Huseyin
authors.initials: H
authors.lastname: Sen
authors.forename: Ertan
authors.initials: E
authors.lastname: Teksoz
authors.forename: Sezai
authors.initials: S
authors.lastname: Ozkan
authors.forename: Guner
authors.initials: G
authors.lastname: Dagli
authors.forename: Ilker
authors.initials: I
authors.lastname: Sucullu
authors.forename: Ali Ilker
authors.initials: AI
authors.lastname: Filiz
authors.forename: Osman Metin
authors.initials: OM
authors.lastname: Ipcioglu
authors.forename: Zafer
authors.initials: Z
authors.lastname: Kucukodaci
mesh_terms: D000779:Anesthetics, Local / Q000008:administration & dosage*; D000818:Animals; D002045:Bupivacaine / Q000008:administration & dosage / Q000031:analogs & derivatives; D005260:Female; D005355:Fibrosis; D006909:Hydroxyproline / Q000378:metabolism; D000077554:Levobupivacaine; D009132:Muscles / Q000378:metabolism / Q000473:pathology; D051381:Rats; D017208:Rats, Wistar; D013718:Tensile Strength; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D000779:Anesthetics, Local; D000077554:Levobupivacaine; D006909:Hydroxyproline; D002045:Bupivacaine