title: Effects of the nanostructure and nanoporosity on bioactive nanohydroxyapatite/reconstituted collagen by electrodeposition.
abstract: Hydroxyapatite (HA)/collagen composites were reported to induce bony growth. Various methods for preparing HA-based composites have been investigated as potential biomaterials for bone substitutes. However, no method can generate a thick nanoporous HA. A novel bone regenerative nanocomposite consisting of nano-hydroxyapatite (HA), nano-amorphous calcium phosphate (ACP) and reconstituted collagen by electrodeposition was designed in this research. Specimens with and without nanoporosity were evaluated using electrochemical measurements, material analyses, and cell-material interactions. The results showed that reconstituted collagen/nano-(HA and ACP) illustrated a multinanoporous structure and enhanced biocompatibility. Nanocomposite was comprised to nano-(HA and ACP) and reconstituted collagen. The core cell structure was formed during electrodeposition. Nanoporosity and nanostructure were observed as formation of nanocomposite. The nano-(HA and ACP) phases were essentially composed of a nanoporous and nanostructural biocomposite. Reconstituted collagen incorporation with the nanoporous and nanostructural biocomposite significantly facilitated the osteogenic differentiation of mesenchymal stem cells. Reconstituted collagen was covered with nano-(HA and ACP), profoundly impacting the enhancement of biocompatibility on application of implant and tissue engineering. The bioactive nano-HA/reconstituted collagen-induced osteogenic differentiation of mesenchymal stem cells enables to enhance bone growth/repair and osseointegration.
authors.affiliation: Graduate Institute of Biomedical Materials and Engineering, College of Oral Medicine, Taipei Medical University, Taipei, Taiwan.
authors.forename: Keng-Liang
authors.initials: KL
authors.lastname: Ou
authors.forename: Jeffery
authors.initials: J
authors.lastname: Wu
authors.forename: Wen-Fu T
authors.initials: WF
authors.lastname: Lai
authors.forename: Charng-Bin
authors.initials: CB
authors.lastname: Yang
authors.forename: Wen-Chang
authors.initials: WC
authors.lastname: Lo
authors.forename: Li-Hsuan
authors.initials: LH
authors.lastname: Chiu
authors.forename: John
authors.initials: J
authors.lastname: Bowley
mesh_terms: D003094:Collagen; D017886:Durapatite; D004563:Electrochemistry; D006801:Humans; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D049329:Nanostructures; D023822:Tissue Engineering; D014961:X-Ray Diffraction
chemical_list: D003094:Collagen; D017886:Durapatite