Use of enzyme phenotypes for identification of meloidogyne species.
Enzyme phenotypes were obtained for 291 populations from 16 species of Meloidogyne originating from 65 countries. Soluble proteins from macerates of individual egg-laying females were separated by electrophoresis in 0.7-mm-thick polyacrylamide gels. Enzymes investigated were nonspecific esterases, malate dehydrogenase, superoxide dismutase, and glutamate-oxaloacetate transaminase. Esterases were polymorphic and most useful in identification of major species. About 94% of the populations of M. hapla, 98% of M. incognita, and 100% of M. javanica could be identified to species on the basis of esterase phenotypes alone. About 84% of the populations of M. arenaria exhibited three distinct phenotypes. Two of them were highly species specific (accuracy of identification 98-100%). The third, and least prevalent, phenotype occurred also in two other species. Another 12 less common Meloidogyne species, of which only one or a few populations of each were studied, exhibited a variety of esterase phenotypes, some of which may prove to be species specific. Superoxide dismutase phenotypes similarly were helpful in the characterization of certain species; however, the same phenotype was often observed in more than one species. The remaining two enzymes, with few exceptions, proved to be less useful for identification of Meloidogyne species. Multienzyme phenotypes represented by two or more enzymes often offered biochemical profiles more valuable for definitive characterization of Meloidogyne species than single enzymes.
Esbenshade
P R
PR
Triantaphyllou
A C
AC