Macrophage inhibitory cytokine MIC-1 is upregulated by short-wavelength light in cultured normal human dermal fibroblasts.
To better understand dermal response to visible light, we used DNA microarray analysis to search genes induced by blue or near-UV light in normal human dermal fibroblasts. Of about 12800 transcripts analyzed, near-UV light most prominently upregulated the transcript level of Mic-1, a gene encoding a TGF-beta superfamily protein. Quantitative RT-PCR and immunoblot analyses revealed that mRNA and protein levels of Mic-1 were upregulated by both short-wavelength light but not by green or red light. These results suggest that the human dermis is a site for macrophage inhibitory cytokine-1 (MIC-1) production and that visible light activates a dermal transcription cascade. Considering the role of MIC-1 in immune regulation and appetite control, photic MIC-1 regulation is of physiological importance.
Akiyama
Masashi
M
Department of Engineering and Bioscience, Graduate School of Advanced Science and Engineering, Waseda University, Tokyo, Japan.
Okano
Keiko
K
Fukada
Yoshitaka
Y
Okano
Toshiyuki
T
D002460:Cell Line; D003513:Cycloheximide / Q000494:pharmacology; D018076:DNA, Complementary / Q000235:genetics; D005347:Fibroblasts; D055436:Growth Differentiation Factor 15 / Q000235:genetics / Q000378:metabolism*; D006262:Health; D006801:Humans; D008027:Light; D020411:Oligonucleotide Array Sequence Analysis; D012333:RNA, Messenger / Q000235:genetics; D012867:Skin / Q000187:drug effects / Q000378:metabolism* / Q000528:radiation effects*; D015854:Up-Regulation / Q000187:drug effects / Q000528:radiation effects*
D018076:DNA, Complementary; D055436:Growth Differentiation Factor 15; D012333:RNA, Messenger; D003513:Cycloheximide