Pentoxifylline modifies three-dimensional collagen lattice model contraction and expression of collagen types I and III by human fibroblasts derived from post-burn hypertrophic scars and from normal skin.
Fibroblasts are thought to be partially responsible for the persisting contractile forces that result in burn contractures. Using a monolayer cell culture and fibroblast populated collagen lattice (FPCL) three-dimensional model we subjected hypertrophic scar and non-cicatricial fibroblasts to the antifibrogenic agent pentoxifylline (PTF - 1mg/mL) in order to reduce proliferation, collagen types I and III synthesis and model contraction. Fibroblasts were isolated from post-burn hypertrophic scars (HSHF) and non-scarred skin (NHF). Cells were grown in monolayers or incorporated into FPCL's and exposed to PTF. In monolayer, cell number proliferation was reduced (46.35% in HSHF group and 37.73% in NHF group, p<0.0001). PTF selectively inhibited collagen III synthesis in the HSHF group while inhibition was more evident to type I collagen synthesis in the NHF group. PTF also reduced contraction in both (HSHF and NHF) FPCL.
Isaac
Cesar
C
Faculty of Medicine, Cell Culture and Wound Healing Research Laboratory, Division of Plastic Surgery, University of São Paulo, Avenida Doutor Arnaldo 455 sala 1360, São Paulo, São Paulo, Brazil. cesaris@plastica.fm.usp.br
Mathor
Mônica Beatriz
MB
Bariani
Giovani
G
Paggiaro
André Oliveira
AO
Herson
Marisa Roma
MR
Goldenstein-Schainberg
Claudia
C
Carrasco
Solange
S
Teodoro
Walcy Rosolia
WR
Yoshinari
Natalino Hajime
NH
Ferreira
Marcus Castro
MC
D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D017439:Cicatrix, Hypertrophic / Q000473:pathology*; D024042:Collagen Type I / Q000096:biosynthesis; D024061:Collagen Type III / Q000096:biosynthesis; D003286:Contracture / Q000473:pathology*; D004353:Drug Evaluation, Preclinical; D005347:Fibroblasts / Q000187:drug effects* / Q000378:metabolism; D006801:Humans; D010431:Pentoxifylline / Q000494:pharmacology*; D010726:Phosphodiesterase Inhibitors / Q000494:pharmacology*; D012867:Skin / Q000166:cytology / Q000187:drug effects / Q000378:metabolism
D024042:Collagen Type I; D024061:Collagen Type III; D010726:Phosphodiesterase Inhibitors; D010431:Pentoxifylline