Efficient enzymatic synthesis of LNA-modified DNA duplexes using KOD DNA polymerase.
Three different LNA-nucleoside triphosphates, LNA-TTP, LNA-ATP and LNA-5-methyl-CTP, were investigated as substrates for KOD DNA polymerase. The results reveal that KOD DNA polymerase is an efficient catalyst for template directed synthesis of DNA oligonucleotide duplexes containing a large number of LNA nucleotides by primer extension reactions. Furthermore, KOD DNA polymerase is shown to be suitable for the PCR amplification of an LNA-modified DNA duplex.
Veedu
Rakesh N
RN
Nucleic Acid Center, Department of Physics and Chemistry, University of Southern Denmark, Campusvej 55, Odense M, Denmark.
Vester
Birte
B
Wengel
Jesper
J
D001483:Base Sequence; D002384:Catalysis; D004247:DNA / Q000737:chemistry* / Q000378:metabolism*; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism*; D008969:Molecular Sequence Data; D009841:Oligonucleotides / Q000737:chemistry* / Q000378:metabolism*
D009841:Oligonucleotides; C477371:locked nucleic acid; D004247:DNA; D004259:DNA-Directed DNA Polymerase