title: Effects of soy phytoestrogens on reference memory and neuronal cholinergic enzymes in ovariectomized rats.
abstract: The effects of soy phytoestrogens on Morris water maze (MWM) performance and neuronal cholinergic enzyme activities and immunoreactivity were studied in ovariectomized (OVX) rats. The rats were assigned to four groups fed control diet (CD), 3.9 mg/kg 17beta-estradiol diet (E2), 263.4 mg/kg soy phytoestrogens diet (SP1), and 526.9 mg/kg soy phytoestrogens diet (SP2). In the MWM task, escape latency and path length were significantly less in the E2 and SP2 groups than in the CD group on the second day. Choline acetyltransferase (ChAT) activity in the cerebral cortex and ChAT immunoreactivity in the diagonal band of Broca were significantly greater in the E2, SP1, and SP2 groups than in the CD group. Acetylcholinesterase activity in the hippocampus in the E2, SP1, and SP2 groups was significantly lower than in the CD group. This study suggests that soy phytoestrogens affect the reference memory and neuronal cholinergic system in OVX rats.
authors.affiliation: Central Research Institute, Dr. Chung's Food Co. Ltd., Cheongju, Republic of Korea.
authors.forename: Yoon-Bok
authors.initials: YB
authors.lastname: Lee
authors.forename: Keun-Ha
authors.initials: KH
authors.lastname: Lee
authors.forename: Heon-Soo
authors.initials: HS
authors.lastname: Sohn
authors.forename: Sung-Joon
authors.initials: SJ
authors.lastname: Lee
authors.forename: Kyung-Hwan
authors.initials: KH
authors.lastname: Cho
authors.forename: Il-Jun
authors.initials: IJ
authors.lastname: Kang
authors.forename: Dong-Woo
authors.initials: DW
authors.lastname: Kim
authors.forename: Yong Kook
authors.initials: YK
authors.lastname: Shin
authors.forename: Tongkun
authors.initials: T
authors.lastname: Pai
authors.forename: In Koo
authors.initials: IK
authors.lastname: Hwang
authors.forename: Moo-Ho
authors.initials: MH
authors.lastname: Won
mesh_terms: D000110:Acetylcholinesterase / Q000378:metabolism*; D000818:Animals; D001835:Body Weight; D001921:Brain / Q000201:enzymology*; D002795:Choline O-Acetyltransferase / Q000378:metabolism*; D004958:Estradiol / Q000494:pharmacology; D005260:Female; D018546:Hypocotyl; D018782:Maze Learning / Q000187:drug effects; D008568:Memory / Q000187:drug effects*; D010052:Ovariectomy; D048789:Phytoestrogens / Q000494:pharmacology*; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013025:Glycine max
chemical_list: D048789:Phytoestrogens; D010936:Plant Extracts; D004958:Estradiol; D002795:Choline O-Acetyltransferase; D000110:Acetylcholinesterase