title: Elimination kinetic of recombinant somatotropin in bovine.
abstract: Bovine somatotropin (bST), also called growth hormone is a protein hormone produced by the pituitary gland and responsible directly or indirectly for various effects on growth, development and reproductive functions. Its recombinant bovine somatotropin form (rbST) is used in dairy cattle to enhance milk production. Even if the effects of treatment with rbST have been largely studied, until now analytical methods able to detect rbST were limited to immunoassays, which suffer from the impossibility to distinguish between the endogenous and the recombinant form. In this study, a sample preparation procedure based on different precipitation steps, extraction on solid phase and enzymatic digestion was used to purify rbST from serum. The detection was performed by liquid chromatography coupled to tandem mass spectrometry in positive electrospray ionization mode (LC-ESI(+)-MS/MS) allowing the unambiguous identification and quantification of rbST in serum. Samples collected from a cow treated with recombinant bovine somatotropin were analysed and for the first time, the elimination kinetic specific to recombinant somatotropin has been characterized in serum. Detection of rbST was possible from 4h 30min to 4 days after administration and concentration was found up to 10ngmL(-1) during the kinetic.
authors.affiliation: Laboratoire d'Etude des Résidus et Contaminants dans les Aliments (LABERCA), Ecole Nationale Vétérinaire de Nantes (ENVN), BP 50707, 44307 Nantes, France.
authors.forename: Marie-Hélène
authors.initials: MH
authors.lastname: Le Breton
authors.forename: Sandrine
authors.initials: S
authors.lastname: Rochereau-Roulet
authors.forename: Gaud
authors.initials: G
authors.lastname: Pinel
authors.forename: Nora
authors.initials: N
authors.lastname: Cesbron
authors.forename: Bruno
authors.initials: B
authors.lastname: Le Bizec
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D013006:Growth Hormone / Q000097:blood* / Q000302:isolation & purification / Q000493:pharmacokinetics; D008969:Molecular Sequence Data; D011994:Recombinant Proteins / Q000097:blood* / Q000302:isolation & purification / Q000493:pharmacokinetics; D052616:Solid Phase Extraction / Q000379:methods; D015813:Substance Abuse Detection / Q000379:methods*; D053719:Tandem Mass Spectrometry / Q000379:methods*; D013997:Time Factors
chemical_list: D011994:Recombinant Proteins; C073294:growth hormone, bovine; D013006:Growth Hormone