Bioactivity of POPs and their effects in mosquitofish in Sydney Olympic Park, Australia.
The site of the 2000 Olympic Games (Sydney Olympic Park (SOP), Sydney, Australia) was contaminated by persistent organic pollutants (POPs) prior to remediation in the 1990s. This study investigates the bioactivity of POPs in the sediment and water of wetlands across SOP by in vitro 2,3,7,8-TCDD equivalence (TCDDeq) measurement (H4IIE cell line bioassay). Further, it examines whether disturbance of these sediments is likely to mobilise ligands for this receptor into the water column. Exposure to aryl hydrocarbon receptor (AhR) ligands was measured in vivo using hepatic cytochrome P4501A (CYP1A) induction (EROD) in the mosquitofish (Gambusia holbrooki). Aqueous TCDDeq ranged from 0.013 to 0.057 pM in SOP wetlands which was significantly (p<0.05) less that in urban reference sites. These concentrations were not correlated to physical or chemical characteristics of the wetlands. In the sediments, TCDDeq ranged from 0.0016 to 7.06 microg/kg and these were not significantly (p>or=0.05) different to that measured in urban reference sites. Simulated disturbance of small quantities of sediment in water samples significantly (p<0.05) increased the levels of TCDDeq measured in the water. Sediment TCDDeq was correlated to sediment SigmaPAH concentration in 2006 and sediment SigmaPCB, SigmaDDT concentrations and fine sediment grain size in 2005. While fish at one SOP wetland had hepatic EROD activity elevated above the estimated basal level for this species, these were at the lower end of the range measured in urban impacted, non-remediated wetlands. EROD activity was positively correlated with both the sediment SigmaPCB load and aqueous TCDDeq. Increased catchment size was correlated with increased EROD activity suggesting an even spread of POPs throughout the residential areas of the Sydney metropolitan area. The concentration of bioactive POPs in the wetlands of SOP is therefore low relative to urban reference sites demonstrating the ongoing success of the remediation program.
Rawson
Christopher A
CA
Institute of Water and Environmental Resource Management (IWERM) and Department of Environmental Sciences, University of Technology, Sydney (UTS), PO Box 123 Broadway, NSW 2001, Australia.
Tremblay
Louis A
LA
Warne
Michael St J
MS
Ying
Guang-Guo
GG
Kookana
Rai
R
Laginestra
Edwina
E
Chapman
John C
JC
Lim
Richard P
RP
D000818:Animals; D001673:Biodegradation, Environmental / Q000187:drug effects; D003532:Cyprinodontiformes / Q000378:metabolism*; D019363:Cytochrome P-450 CYP1A1 / Q000032:analysis; D004781:Environmental Exposure / Q000009:adverse effects / Q000032:analysis; D004784:Environmental Monitoring; D052918:Environmental Restoration and Remediation; D019015:Geologic Sediments / Q000737:chemistry*; D009517:New South Wales; D000072317:Polychlorinated Dibenzodioxins / Q000032:analysis; D018336:Receptors, Aryl Hydrocarbon / Q000032:analysis / Q000378:metabolism; D045483:Rivers / Q000737:chemistry*; D014874:Water Pollutants, Chemical / Q000032:analysis* / Q000378:metabolism; D053833:Wetlands
D000072317:Polychlorinated Dibenzodioxins; D018336:Receptors, Aryl Hydrocarbon; D014874:Water Pollutants, Chemical; D019363:Cytochrome P-450 CYP1A1