title: Improvement of arachidonic acid and eicosapentaenoic acid production by increasing the copy number of the genes encoding fatty acid desaturase and elongase into Pichia pastoris.
abstract: Genes encoding Delta6 desaturase, Delta6 fatty acid elongase, and Delta5 desaturase from the alga, Phaeodactylum tricornutum, were co-expressed in Pichia pastoris to produce arachidonic acid (ARA; 20:4 Delta(5, 8, 11, 14)) and eicosapentaenoic acid (EPA; 20:5 Delta(5, 8, 11, 14, 17)). A panel of Pichia clones carrying progressively increasing copies of the heterologous gene expression cassette was created using an in vitro multimerization approach. ARA and EPA accumulated up to 0.3 and 0.1% of total fatty acids, respectively, in the recombinant P. pastoris carrying with double copies of these three heterologous genes, as compared to 0.1 and 0.05%, respectively, in the recombinant P. pastoris with single copy.
authors.affiliation: Institute of Resource Biology and Biotechnology, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, 430074, China.
authors.forename: Yun-Tao
authors.initials: YT
authors.lastname: Li
authors.forename: Mao-Teng
authors.initials: MT
authors.lastname: Li
authors.forename: Chu-Hua
authors.initials: CH
authors.lastname: Fu
authors.forename: Peng-Peng
authors.initials: PP
authors.lastname: Zhou
authors.forename: Jian-Min
authors.initials: JM
authors.lastname: Liu
authors.forename: Long-Jiang
authors.initials: LJ
authors.lastname: Yu
mesh_terms: D000123:Acetyltransferases / Q000235:genetics* / Q000378:metabolism; D016718:Arachidonic Acid / Q000096:biosynthesis*; D017377:Diatoms / Q000201:enzymology / Q000235:genetics; D015118:Eicosapentaenoic Acid / Q000096:biosynthesis*; D044943:Fatty Acid Desaturases / Q000235:genetics* / Q000378:metabolism; D000081031:Fatty Acid Elongases; D018628:Gene Dosage; D005800:Genes, Fungal; D010843:Pichia / Q000201:enzymology* / Q000378:metabolism*; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D011994:Recombinant Proteins; D016718:Arachidonic Acid; D015118:Eicosapentaenoic Acid; D044943:Fatty Acid Desaturases; D000123:Acetyltransferases; D000081031:Fatty Acid Elongases