title: Identification of low abundance polyA-binding proteins in Arabidopsis chloroplast using polyA-affinity column.
abstract: Proteins could be well separated and further identified by the use of 2-DE and related techniques. Yet, there are many proteins could not be detected even by more effective dyes because of their inherent low abundance or their low resolution. As a result, polyA-affinity column was used as a method to enrich polyA-binding proteins and then identified by MALDI-TOF-MS. In this study, 23 Arabidopsis chloroplast protein spots coded by 18 genes were identified, and majority of these proteins were classified into three related categories according to their annotations in the Swiss-Prot database, including NAD-, RNA-, and ATP-binding motifs, respectively. The major goal of the present Arabidopsis chloroplast proteomics project was to identify novel polyA-binding proteins or protein isoforms located in Arabidopsis chloroplasts and the specific research of cellular proteins with extremely low transcription levels could be fulfilled.
authors.affiliation: Key Laboratory of Forest Tree Genetic Improvement and Biotechnology, Ministry of Education, Northeast Forestry University, 150040, Harbin, China.
authors.forename: Rui-Juan
authors.initials: RJ
authors.lastname: Ni
authors.forename: Zhuo
authors.initials: Z
authors.lastname: Shen
authors.forename: Chuan-Ping
authors.initials: CP
authors.lastname: Yang
authors.forename: Ya-Dan
authors.initials: YD
authors.lastname: Wu
authors.forename: Ying-Dong
authors.initials: YD
authors.lastname: Bi
authors.forename: Bai-Chen
authors.initials: BC
authors.lastname: Wang
mesh_terms: D017360:Arabidopsis / Q000737:chemistry / Q000378:metabolism*; D002736:Chloroplasts / Q000737:chemistry / Q000378:metabolism*; D002846:Chromatography, Affinity / Q000379:methods*; D015180:Electrophoresis, Gel, Two-Dimensional; D009994:Osmolar Concentration; D010940:Plant Proteins / Q000032:analysis / Q000302:isolation & purification* / Q000378:metabolism; D011061:Poly A / Q000378:metabolism; D039102:Poly(A)-Binding Proteins / Q000032:analysis / Q000302:isolation & purification* / Q000378:metabolism; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D010940:Plant Proteins; D039102:Poly(A)-Binding Proteins; D011061:Poly A