title: Simultaneous saccharification and fermentation of lignocellulosic residues pretreated with phosphoric acid-acetone for bioethanol production.
abstract: Bermudagrass, reed and rapeseed were pretreated with phosphoric acid-acetone and used for ethanol production by means of simultaneous saccharification and fermentation (SSF) with a batch and fed-batch mode. When the batch SSF experiments were conducted in a 3% low effective cellulose, about 16 g/L of ethanol were obtained after 96 h of fermentation. When batch SSF experiments were conducted with a higher cellulose content (10% effective cellulose for reed and bermudagrass and 5% for rapeseed), higher ethanol concentrations and yields (of more than 93%) were obtained. The fed-batch SSF strategy was adopted to increase the ethanol concentration further. When a higher water-insoluble solid (up to 36%) was applied, the ethanol concentration reached 56 g/L of an inhibitory concentration of the yeast strain used in this study at 38 degrees C. The results show that the pretreated materials can be used as good feedstocks for bioethanol production, and that the phosphoric acid-acetone pretreatment can effectively yield a higher ethanol concentration.
authors.affiliation: Biochemical Engineering Lab, Department of Chemical and Biomolecular Engineering, KAIST, Yuseong-gu, Daejeon, Republic of Korea.
authors.forename: Hui
authors.initials: H
authors.lastname: Li
authors.forename: Nag-Jong
authors.initials: NJ
authors.lastname: Kim
authors.forename: Min
authors.initials: M
authors.lastname: Jiang
authors.forename: Jong Won
authors.initials: JW
authors.lastname: Kang
authors.forename: Ho Nam
authors.initials: HN
authors.lastname: Chang
mesh_terms: D000096:Acetone / Q000737:chemistry; D018533:Biomass; D019149:Bioreactors; D001709:Biotechnology / Q000379:methods*; D002241:Carbohydrates / Q000737:chemistry; D002482:Cellulose / Q000737:chemistry / Q000378:metabolism*; D004736:Energy-Generating Resources; D000431:Ethanol / Q000737:chemistry / Q000378:metabolism*; D005285:Fermentation; D006868:Hydrolysis; D008031:Lignin / Q000737:chemistry; D010756:Phosphoric Acids / Q000737:chemistry; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D013696:Temperature; D013997:Time Factors
chemical_list: D002241:Carbohydrates; D010756:Phosphoric Acids; C036909:lignocellulose; D000096:Acetone; D000431:Ethanol; D002482:Cellulose; D008031:Lignin; C030242:phosphoric acid