Adapted liquid crystal display backlighting unit for densitometry of stained polyacrylamide electrophoresis gels.
Appropriate monochromatic illumination that is spatially uniform will ensure densitometry of stained polyacrylamide electrophoresis gels with high sensitivity and repeatability. The approach of using an array of LEDs as an illuminator has practical limitations. Here we describe an alternative method based on adapted liquid crystal display backlighting. By changing the fluorescent light source and using a region where light intensity is uniform, we demonstrate the ability to achieve highly sensitive and repeatable densitometry measurements of polyacrylamide electrophoresis gels with Coomassie-stained proteins.
Tan
Han Yen
HY
Department of Mechanical & Aerospace Engineering, Monash University, VIC, Australia.
Ng
Tuck Wah
TW
Liew
Oi Wah
OW
D003720:Densitometry / Q000295:instrumentation*; D004591:Electrophoresis, Polyacrylamide Gel; D008029:Lighting; D016014:Linear Models; D050866:Liquid Crystals; D015203:Reproducibility of Results; D012394:Rosaniline Dyes / Q000737:chemistry; D012680:Sensitivity and Specificity
D012394:Rosaniline Dyes; C048139:Coomassie blue