Genetic and epidemiological characterization of Stretch Lagoon orbivirus, a novel orbivirus isolated from Culex and Aedes mosquitoes in northern Australia.
Stretch Lagoon orbivirus (SLOV) was isolated in 2002 from pooled Culex annulirostris mosquitoes collected at Stretch Lagoon, near the Wolfe Creek national park in the Kimberley region of Western Australia. Conventional serological tests were unable to identify the isolate, and electron microscopy indicated a virus of the genus Orbivirus, family Reoviridae. Here, a cDNA subtraction method was used to obtain approximately one-third of the viral genome, and further sequencing was performed to complete the sequences of segment 1 (viral polymerase) and segment 2 (conserved inner-core protein). Phylogenetic analysis showed that SLOV should be considered a new species within the genus Orbivirus. A real-time RT-PCR test was designed to study the epidemiology of SLOV in the field. Six additional isolates of SLOV were identified, including isolates from four additional locations and two additional mosquito species. Horses, donkeys and goats were implicated as potential vertebrate hosts in a serological survey.
Cowled
Chris
C
CSIRO Livestock Industries, Australian Animal Health Laboratory, East Geelong, VIC 3220, Australia.
Palacios
Gustavo
G
Center for Infection and Immunity, Mailman School of Public Health, Columbia University, New York, NY, USA.
Melville
Lorna
L
Northern Territory Department of Primary Industries, Fisheries and Mines, Berrimah Veterinary Laboratories, Berrimah, Northern Territory 0801, Australia.
Weir
Richard
R
Northern Territory Department of Primary Industries, Fisheries and Mines, Berrimah Veterinary Laboratories, Berrimah, Northern Territory 0801, Australia.
Walsh
Susan
S
Northern Territory Department of Primary Industries, Fisheries and Mines, Berrimah Veterinary Laboratories, Berrimah, Northern Territory 0801, Australia.
Davis
Steven
S
Northern Territory Department of Primary Industries, Fisheries and Mines, Berrimah Veterinary Laboratories, Berrimah, Northern Territory 0801, Australia.
Gubala
Aneta
A
CSIRO Livestock Industries, Australian Animal Health Laboratory, East Geelong, VIC 3220, Australia.
Lipkin
W Ian
WI
Center for Infection and Immunity, Mailman School of Public Health, Columbia University, New York, NY, USA.
Briese
Thomas
T
Center for Infection and Immunity, Mailman School of Public Health, Columbia University, New York, NY, USA.
Boyle
David
D
CSIRO Livestock Industries, Australian Animal Health Laboratory, East Geelong, VIC 3220, Australia.
D000330:Aedes / Q000821:virology*; D000818:Animals; D001315:Australia; D001483:Base Sequence; D002460:Cell Line; D016000:Cluster Analysis; D006224:Cricetinae; D003465:Culex / Q000821:virology*; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D008969:Molecular Sequence Data; D018163:Orbivirus / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D010802:Phylogeny; D012367:RNA, Viral / Q000235:genetics*; D012088:Reoviridae Infections / Q000453:epidemiology / Q000662:veterinary*; D017422:Sequence Analysis, DNA; D017385:Sequence Homology
D018076:DNA, Complementary; D012367:RNA, Viral