Application of retrovirus-mediated expression cloning for receptor screening of a parasite.
Retrovirus-mediated expression cloning has been applied in both virology and cell biology. Although there is some difficulty in applying this technique to screening for a receptor recognized by an intracellular parasite, we modified the conventional method to identify a putative receptor for the Plasmodium falciparum BAEBL protein. We show that this method is effective in screening for a parasite receptor.
Kobayashi
Kyousuke
K
Department of Veterinary Microbiology, Graduate School of Agricultural and Life Sciences, University of Tokyo, Bunkyo-ku, Tokyo 113-8657, Japan.
Kato
Kentaro
K
Sugi
Tatsuki
T
Yamane
Daisuke
D
Shimojima
Masayuki
M
Tohya
Yukinobu
Y
Akashi
Hiroomi
H
D000818:Animals; D002352:Carrier Proteins / Q000378:metabolism*; D002460:Cell Line; D003001:Cloning, Molecular / Q000379:methods*; D005434:Flow Cytometry; D053673:Glypicans / Q000302:isolation & purification / Q000378:metabolism*; D006801:Humans; D008565:Membrane Proteins; D008954:Models, Biological; D010963:Plasmodium falciparum / Q000378:metabolism*; D011485:Protein Binding; D015800:Protozoan Proteins / Q000378:metabolism*; D012190:Retroviridae / Q000235:genetics*
D002352:Carrier Proteins; D053673:Glypicans; D008565:Membrane Proteins; D015800:Protozoan Proteins; C451423:erythrocyte binding protein-2, Plasmodium falciparum