title: Multiplex polymerase chain reaction (PCR) and real-time multiplex PCR for the simultaneous detection of plant viruses.
abstract: Multiplex Polymerase Chain Reaction (PCR) can be used for the simultaneous detection of plant viruses. Multiple primer pairs or polyvalent primer pairs can be used to detect and identify several viruses in a single PCR.
authors.affiliation: IBMCP, Universidad Politecnica de Valencia-Consejo Superior de Investigaciones Cientificas, CPI, Valencia, Spain.
authors.forename: V
authors.initials: V
authors.lastname: Pallas
authors.forename: J
authors.initials: J
authors.lastname: Sanchez-Navarro
authors.forename: A
authors.initials: A
authors.lastname: Varga
authors.forename: F
authors.initials: F
authors.lastname: Aparicio
authors.forename: D
authors.initials: D
authors.lastname: James
mesh_terms: D019411:Clinical Laboratory Techniques; D017931:DNA Primers; D004279:DNA, Viral / Q000032:analysis*; D005453:Fluorescence; D010942:Plant Viruses / Q000235:genetics / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000379:methods*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*
chemical_list: D017931:DNA Primers; D004279:DNA, Viral