Chromato-panning: an efficient new mode of identifying suitable ligands from phage display libraries.
BACKGROUND
Phage Display technology is a well established technique for high throughput screening of affinity ligands. Here we describe a new compact chromato-panning procedure for selection of suitable binders from a phage peptide display library.


RESULTS
Both phages and E. coli cells pass non-hindered through the interconnected pores of macroporous gel, so called cryogel. After coupling a ligand to a monolithic cryogel column, the phage library was applied on the column and non-bound phages were washed out. The selection of strong phage-binders was achieved already after the first panning cycle due to the efficient separation of phage-binders from phage-non-binders in chromatographic mode rather than in batch mode as in traditional biopanning procedures. E. coli cells were applied on the column for infection with the specifically bound phages.


CONCLUSIONS
Chromato-panning allows combining several steps of the panning procedure resulting in 4-8 fold decrease of total time needed for phage selection.
Noppe
Wim
W
Department of Biotechnology, Lund University, Lund, Sweden. Wim.Noppe@kuleuven-kortrijk.be
Plieva
Fatima
F
Galaev
Igor Yu
IY
Pottel
Hans
H
Deckmyn
Hans
H
Mattiasson
Bo
B
D003080:Cold Temperature; D004797:Enzyme-Linked Immunosorbent Assay; D004926:Escherichia coli / Q000378:metabolism; D020100:Hydrogels / Q000378:metabolism*; D007781:Lactoferrin / Q000378:metabolism*; D008024:Ligands; D019151:Peptide Library; D010455:Peptides / Q000302:isolation & purification*; D013379:Substrate Specificity
D020100:Hydrogels; D008024:Ligands; D019151:Peptide Library; D010455:Peptides; D007781:Lactoferrin