Cloning and in situ expression studies of the Hydrogenobaculum arsenite oxidase genes.
Novel arsenite [As(III)] oxidase structural genes (aoxAB) were cloned from Hydrogenobaculum bacteria isolated from an acidic geothermal spring. Reverse transcriptase PCR demonstrated expression throughout the outflow channel, and the aoxB cDNA clones exhibited distribution patterns relative to the physicochemical gradients in the spring. Microelectrode analyses provided evidence of quantitative As(III) transformation within the microbial mat.
Clingenpeel
Scott R
SR
Thermal Biology Institute and Department of Land Resources & Environmental Sciences, Montana State University, Bozeman, MT 59717, USA.
D'Imperio
Seth
S
Oduro
Harry
H
Druschel
Greg K
GK
McDermott
Timothy R
TR
D001419:Bacteria / Q000235:genetics* / Q000302:isolation & purification; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism*; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D020869:Gene Expression Profiling; D045482:Hot Springs / Q000382:microbiology*; D008839:Microelectrodes; D008969:Molecular Sequence Data; D010088:Oxidoreductases / Q000235:genetics* / Q000378:metabolism*; D010802:Phylogeny; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D017385:Sequence Homology
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D010088:Oxidoreductases; C078179:arsenite oxidase