Rapid and label-free bacteria detection by surface plasmon resonance (SPR) biosensors.
Surface Plasmon Resonance (SPR) biosensor technology has been successfully used for the detection of various analytes such as proteins, drugs, DNA, and microorganisms. SPR-based immunosensors that coupled with a specific antigen-antibody reaction, have become a promising tool for the quantification of bacteria as it offers sensitive, specific, rapid, and label-free detection. In this paper, we review the important issues in the development of SPR-based immunoassays for bacteria detection, concentrating on instrumentation, surface functionalization, liquid handling, and surface regeneration. In addition, this review touches on the recent advances in SPR biosensing for sensitivity enhancement.
Dudak
Fahriye Ceyda
FC
Department of Food Engineering, Hacettepe University, Beytepe, Ankara, Turkey.
Boyaci
Ismail Hakki
IH
D055910:Antibodies, Immobilized; D001419:Bacteria / Q000302:isolation & purification; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D017743:Equipment Reuse; D020349:Surface Plasmon Resonance / Q000295:instrumentation / Q000379:methods*
D055910:Antibodies, Immobilized