title: Centrosome abnormalities during a Chlamydia trachomatis infection are caused by dysregulation of the normal duplication pathway.
abstract: The presence of supernumerary centrosomes in cells infected with Chlamydia trachomatis may provide a mechanism to explain the association of C. trachomatis genital infection with cervical cancer. We show that the amplified centrosomal foci induced during a chlamydial infection contain both centriolar and pericentriolar matrix markers, demonstrating that they are bona fide centrosomes. As there were multiple immature centrioles but approximately one mature centriole per cell, aborted cytokinesis alone cannot account for centrosome amplification during a chlamydial infection. Production of supernumerary centrosomes required the kinase activities of Cdk2 and Plk4, which are known regulators of centrosome duplication, and progression through S-phase, which is the stage in the cell cycle when duplication of the centrosome occurs. These requirements indicate that centrosome amplification during a chlamydial infection depends on the host centrosome duplication pathway, which normally produces a single procentriole from each template centriole. However, C. trachomatis induces a loss of numerical control so that multiple procentrioles are formed per template.
authors.affiliation: Departments of Developmental and Cell Biology, University of California Irvine, 92697-2300, USA.
authors.forename: Kirsten A
authors.initials: KA
authors.lastname: Johnson
authors.forename: Ming
authors.initials: M
authors.lastname: Tan
authors.forename: Christine
authors.initials: C
authors.lastname: Sütterlin
mesh_terms: D002453:Cell Cycle; D002502:Centrioles / Q000378:metabolism; D018385:Centrosome / Q000378:metabolism*; D002692:Chlamydia trachomatis / Q000502:physiology*; D051357:Cyclin-Dependent Kinase 2 / Q000378:metabolism; D004261:DNA Replication; D006367:HeLa Cells; D006801:Humans; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism
chemical_list: C490219:PLK4 protein, human; D017346:Protein Serine-Threonine Kinases; C495894:CDK2 protein, human; D051357:Cyclin-Dependent Kinase 2