title: Linear and accurate quantitation of proenkephalin-derived peptides by isotopic labeling with internal standards and mass spectrometry.
abstract: Proenkephalin (PE) represents the precursor protein of the active peptide neurotransmitter enkephalin. Quantitative analysis of peptides and proteins is an objective of mass spectrometry-based studies of biological systems and will be important for studying the proteolytic conversion of proproteins to active enkephalin and neuropeptides. The goal of this study was to define and optimize quantitation of different amounts of tryptic peptides derived from PE using light (H4, 4 hydrogens) and heavy (D4, 4 deuteriums) succinic anhydride for isotopic labeling of peptides analyzed by liquid chromatography-tandem mass spectrometry (LC-MS/MS). Comparisons were made between PE-derived peptides with and without internal standards. Importantly, incorporation of internal standards of known amounts of heavy isotope-labeled tryptic peptides of PE provided linear calibration plots with accurate quantitation. In contrast, comparison of light and heavy isotope-labeled peptides without internal standards produced variable and inaccurate nonlinear isotopic ratio comparisons of PE-derived peptides. These results demonstrate that use of internal standards composed of a defined amount(s) of standard peptides (PE-derived tryptic peptides) is necessary for high-quality linear quantitation of peptides by isotopic labeling and MS/MS.
authors.affiliation: Skaggs School of Pharmacy and Pharmaceutical Sciences, University of California, 9500 Gilman Drive, MC-744, San Diego, La Jolla, CA 92093, USA. sbark@ucsd.edu
authors.forename: Steven J
authors.initials: SJ
authors.lastname: Bark
authors.forename: Weiya D
authors.initials: WD
authors.lastname: Lu
authors.forename: Vivian
authors.initials: V
authors.lastname: Hook
mesh_terms: D002853:Chromatography, Liquid; D004745:Enkephalins / Q000032:analysis / Q000737:chemistry*; D006801:Humans; D007553:Isotope Labeling; D010455:Peptides / Q000032:analysis / Q000737:chemistry*; D011498:Protein Precursors / Q000032:analysis / Q000737:chemistry*; D012015:Reference Standards; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D004745:Enkephalins; D010455:Peptides; D011498:Protein Precursors; C029992:proenkephalin