Quantitative analysis of fecal sapovirus shedding: identification of nucleotide substitutions in the capsid protein during prolonged excretion.
Sapovirus (SaV) is an important pathogen causing gastroenteritis in humans. Quantitative analysis of the viral loads in feces collected from two SaV outbreaks was performed. Our results showed that SaV excretion generally decreased to an undetectable level in 2 weeks; however, some individuals excrete SaV in feces at high concentrations for 2-4 weeks after the onset of illness. In addition, we identified for the first time nucleotide changes in the capsid region during prolonged excretion.
Iwakiri
Akira
A
Miyazaki Prefectural Institute for Public Health and Environment, Japan.
Ganmyo
Hidenari
H
Yamamoto
Seigo
S
Otao
Kayoko
K
Mikasa
Mieko
M
Kizoe
Sigeko
S
Katayama
Kazuhiko
K
Wakita
Takaji
T
Takeda
Naokazu
N
Oka
Tomoichiro
T
D000293:Adolescent; D000328:Adult; D017250:Caliciviridae Infections / Q000821:virology*; D036022:Capsid Proteins / Q000235:genetics*; D002648:Child; D005243:Feces / Q000821:virology*; D005260:Female; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D010802:Phylogeny; D017354:Point Mutation; D029341:Sapovirus / Q000235:genetics* / Q000302:isolation & purification*; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D017201:Virus Shedding; D055815:Young Adult
D036022:Capsid Proteins