title: Neuroplasticity and neuroprotection in enteric neurons: role of epithelial cells.
abstract: Neurons of enteric nervous system (ENS) regulate intestinal epithelial cells (IEC) functions but whether IEC can impact upon the neurochemical coding and survival of enteric neurons remain unknown. Neuro-epithelial interactions were studied using a coculture model composed of IEC lines and primary culture of rat ENS or human neuroblastoma cells (SH-SY5Y). Neurochemical coding of enteric neurons was analysed by immunohistochemistry and quantitative PCR. Neuroprotective effects of IEC were tested by measuring neuron specific enolase (NSE) release or cell permeability to 7-amino-actinomycin D (7-AAD). Following coculture with IEC, the percentage of VIP-immunoreactive (IR) neurons but not NOS-IR and VIP mRNA expression were significantly increased. IEC significantly reduced dopamine-induced NSE release and 7-AAD permeability in culture of ENS and SH-SY5Y, respectively. Finally, we showed that NGF had neuroprotective effects but reduced VIP expression in enteric neurons. In conclusion, our study identified a novel role for IEC in the regulation of enteric neuronal properties.
authors.affiliation: INSERM, U913, 1, Place Alexis Ricordeau - 3HNB, Nantes F-44093, France.
authors.forename: Raphaël
authors.initials: R
authors.lastname: Moriez
authors.forename: Hind
authors.initials: H
authors.lastname: Abdo
authors.forename: Tanguy
authors.initials: T
authors.lastname: Chaumette
authors.forename: Magali
authors.initials: M
authors.lastname: Faure
authors.forename: Bernard
authors.initials: B
authors.lastname: Lardeux
authors.forename: Michel
authors.initials: M
authors.lastname: Neunlist
mesh_terms: D000818:Animals; D003609:Dactinomycin / Q000031:analogs & derivatives / Q000378:metabolism; D005456:Fluorescent Dyes / Q000378:metabolism; D006801:Humans; D007413:Intestinal Mucosa / Q000502:physiology*; D007422:Intestines / Q000166:cytology / Q000294:innervation* / Q000502:physiology; D009473:Neuronal Plasticity; D009474:Neurons / Q000201:enzymology / Q000502:physiology*; D010751:Phosphopyruvate Hydratase / Q000378:metabolism; D051381:Rats
chemical_list: D005456:Fluorescent Dyes; D003609:Dactinomycin; C025942:7-aminoactinomycin D; D010751:Phosphopyruvate Hydratase