title: Peptide-imprinted polymer microspheres prepared by precipitation polymerization using a single bi-functional monomer.
abstract: A single bi-functional monomer, N,O-bismethacryloyl ethanolamine (NOBE), was used in precipitation polymerization system to synthesize molecularly imprinted polymer (MIP) microspheres. Highly specific binding sites were obtained for N-terminal protected neuropeptides, Boc-Leu-enkephalin and Pyr-Leu-enkephalin. The use of NOBE allowed binding sites to be formed in polymer microspheres that are able to recognize target peptides through the consensus C-terminal sequence. The interesting molecular binding results suggest a new approach for peptide analysis combining in situ chemical modification with MIP recognition under non-aqueous conditions.
authors.affiliation: Pure and Applied Biochemistry, Chemical Center, Lund University, Box 124, S-221 00 Lund, Sweden.
authors.forename: Keiichi
authors.initials: K
authors.lastname: Yoshimatsu
authors.forename: Jason
authors.initials: J
authors.lastname: LeJeune
authors.forename: David A
authors.initials: DA
authors.lastname: Spivak
authors.forename: Lei
authors.initials: L
authors.lastname: Ye
mesh_terms: D000818:Animals; D040761:Biomimetic Materials; D011232:Chemical Precipitation; D008863:Microspheres; D054802:Molecular Imprinting; D010455:Peptides / Q000032:analysis* / Q000737:chemistry; D016062:Porosity; D013499:Surface Properties
chemical_list: D010455:Peptides