title: Cloning and in situ expression studies of the Hydrogenobaculum arsenite oxidase genes.
abstract: Novel arsenite [As(III)] oxidase structural genes (aoxAB) were cloned from Hydrogenobaculum bacteria isolated from an acidic geothermal spring. Reverse transcriptase PCR demonstrated expression throughout the outflow channel, and the aoxB cDNA clones exhibited distribution patterns relative to the physicochemical gradients in the spring. Microelectrode analyses provided evidence of quantitative As(III) transformation within the microbial mat.
authors.affiliation: Thermal Biology Institute and Department of Land Resources & Environmental Sciences, Montana State University, Bozeman, MT 59717, USA.
authors.forename: Scott R
authors.initials: SR
authors.lastname: Clingenpeel
authors.forename: Seth
authors.initials: S
authors.lastname: D'Imperio
authors.forename: Harry
authors.initials: H
authors.lastname: Oduro
authors.forename: Greg K
authors.initials: GK
authors.lastname: Druschel
authors.forename: Timothy R
authors.initials: TR
authors.lastname: McDermott
mesh_terms: D001419:Bacteria / Q000235:genetics* / Q000302:isolation & purification; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism*; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D020869:Gene Expression Profiling; D045482:Hot Springs / Q000382:microbiology*; D008839:Microelectrodes; D008969:Molecular Sequence Data; D010088:Oxidoreductases / Q000235:genetics* / Q000378:metabolism*; D010802:Phylogeny; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D017385:Sequence Homology
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D010088:Oxidoreductases; C078179:arsenite oxidase