Introducing capillary electrophoresis with laser-induced fluorescence detection (CE-LIF) for the characterization of konjac glucomannan oligosaccharides and their in vitro fermentation behavior.
The application of capillary electrophoresis with laser-induced fluorescence detection (CE-LIF) as a tool for the characterization of complex carbohydrate structures was investigated for konjac glucomannan (KGM) oligosaccharide mixtures and the monitoring of their structural changes during 72 h of in vitro fermentation with human gut flora. Different types of KGM oligosaccharide mixtures were produced from a KGM polysaccharide using endo-beta-(1,4)-mannanase and endo-beta-(1,4)-glucanase. Distinction of structures emerging from different enzymatic KGM digests and detection of acetylated oligosaccharides were possible by both CE-LIF and matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry (MALDI-TOF MS). Using CE-LIF it could be shown that the endo-beta-(1,4)-glucanase digest exhibited a large degradability of the DP2, DP3, DP5, and DP6 components during in vitro fermentation, whereas the endo-beta-(1,4)-mannanase digest was digested only slightly, thereby highlighting the influence of structural characteristics on the fermentability by human gut flora.
Albrecht
Simone
S
Department of Agrotechnology and Food Sciences, Laboratory of Food Chemistry, Wageningen University, Bomenweg 2, 6703 HD Wageningen, The Netherlands.
van Muiswinkel
Gonny C J
GC
Schols
Henk A
HA
Voragen
Alphons G J
AG
Gruppen
Harry
H
D002480:Cellulase / Q000378:metabolism; D019075:Electrophoresis, Capillary / Q000379:methods*; D005285:Fermentation; D006801:Humans; D007422:Intestines / Q000382:microbiology; D008351:Mannans / Q000737:chemistry*; D008361:Mannosidases / Q000378:metabolism; D009844:Oligosaccharides / Q000737:chemistry* / Q000378:metabolism*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D008351:Mannans; D009844:Oligosaccharides; C022901:(1-6)-alpha-glucomannan; D008361:Mannosidases; D002480:Cellulase; C041867:endo-1,4-beta-D-mannanase