Thin-layer chromatography for the identification and semi-quantification of biogenic amines produced by bacteria.
The screening TLC method described enables the simultaneous identification and semi-quantification of eight biogenic amines with a large number of samples handled in a short period of time. The dansylation process time was drastically reduced from 80 to 18 min by increasing the reaction temperature to 100 degrees C. Bacteria could be classified as no, low, moderate or powerful amine producers when no spots were noticeable in TLC plates, less than 50 mg/L, from 50 to 500 mg/L, or more than 500 mg/L of amines were present in the decarboxylase broth medium, respectively. This TLC method is a simpler, faster and less expensive alternative to other methods, such as differential culture media, HPLC and even more sensitive than other TLC procedures.
Latorre-Moratalla
M L
ML
Department of Nutrition and Food Science, Faculty of Pharmacy, University of Barcelona, Avinguda Joan XXIII s/n, E-08028 Barcelona, Spain.
Bover-Cid
S
S
Veciana-Nogués
T
T
Vidal-Carou
M C
MC
D001419:Bacteria / Q000737:chemistry / Q000378:metabolism*; D001679:Biogenic Amines / Q000737:chemistry* / Q000378:metabolism*; D002855:Chromatography, Thin Layer / Q000379:methods*
D001679:Biogenic Amines