title: Engineered luciferases for molecular sensing in living cells.
abstract: As a means for visualizing molecular physiology within living cells, new strategies are emerging for engineering luciferases into intracellular biosensors. These biosensors can be classified as bimolecular, relying on complementation of luciferase fragments, or intramolecular, relying on domain insertion within the luciferase structure. Multiple design strategies have recently surfaced for the development of intramolecular sensors, allowing dynamic detection of small molecules or post-translational modifications within cells. Building on successes achieved in cell culture, these sensors are now beginning to reveal molecular processes within living organisms.
authors.affiliation: Promega Corporation, Madison, WI 53711, USA.
authors.forename: Brock
authors.initials: B
authors.lastname: Binkowski
authors.forename: Frank
authors.initials: F
authors.lastname: Fan
authors.forename: Keith
authors.initials: K
authors.lastname: Wood
mesh_terms: D000818:Animals; D015374:Biosensing Techniques / Q000379:methods*; D045744:Cell Line, Tumor; D001517:Coleoptera; D000242:Cyclic AMP / Q000378:metabolism; D006801:Humans; D008156:Luciferases / Q000235:genetics / Q000378:metabolism*; D008163:Luminescent Measurements / Q000379:methods; D015202:Protein Engineering; D011499:Protein Processing, Post-Translational
chemical_list: D000242:Cyclic AMP; D008156:Luciferases