title: [Influence of electromagnetic radiation on raf kinase inhibitor protein and its related proteins of hippocampus].
abstract: OBJECTIVE
  To study the development of changes for Raf kinase inhibitor protein (RKIP) and its mRNA in rats hippocampus after electromagnetic radiation.
  
  
  METHODS
  Rats were exposed to X-band high power microwave (X-HPM), S-band high power microwave (S-HPM) and electromagnetic pulse (EMP) radiation source respectively. The animal model of electromagnetic radiation was established. Western blot was used to detect the expression of RKIP, and RT-PCR was applied to detect the expression of RKIP mRNA. The interaction of RKIP and Raf-1 was measured with co-immunoprecipitation method, and the expression of cerebral choline acetyltransferase (CHAT) was measured by immunohistochemistry.
  
  
  RESULTS
  The expression of RKIP significantly down-regulated at 6 h after radiation, and recovered at 1 d in group EMP, but the down-regulation continued during 1 approximately 7 d after radiation in the two microwave groups. The expression of RKIP mRNA changed wavily during 6 h approximately 7 d after radiation, which showed down-regulation at 6 h, and up-regulation at 3 d. The interaction of RKIP and Raf-1 decreased during 6 h approximately 7 d after radiation, most significantly at 7 d, and the two microwave groups were more significant. The expression of CHAT decreased continuously during 6 h approximately 7 d after radiation, and generally recovered on 14 d.
  
  
  CONCLUSIONS
  The down-regulation of RKIP and its related proteins of hippocampus is induced by electromagnetic radiation.
authors.affiliation: Institute of Radiation Medicine, Academy of Military Medical Science, Beijing 100850, China.
authors.forename: Hong-yan
authors.initials: HY
authors.lastname: Zuo
authors.forename: De-wen
authors.initials: DW
authors.lastname: Wang
authors.forename: Rui-yun
authors.initials: RY
authors.lastname: Peng
authors.forename: Shui-ming
authors.initials: SM
authors.lastname: Wang
authors.forename: Ya-bing
authors.initials: YB
authors.lastname: Gao
authors.forename: Xin-ping
authors.initials: XP
authors.lastname: Xu
authors.forename: Jun-jie
authors.initials: JJ
authors.lastname: Ma
mesh_terms: D000818:Animals; D060733:Electromagnetic Radiation; D006624:Hippocampus / Q000378:metabolism / Q000528:radiation effects; D020930:MAP Kinase Kinase Kinases / Q000378:metabolism; D008297:Male; D049488:Phosphatidylethanolamine Binding Protein / Q000235:genetics / Q000378:metabolism*; D019908:Proto-Oncogene Proteins c-raf; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D017208:Rats, Wistar
chemical_list: C550693:PEBP1 protein, rat; D049488:Phosphatidylethanolamine Binding Protein; D012333:RNA, Messenger; D019908:Proto-Oncogene Proteins c-raf; C546782:Raf1 protein, rat; D020930:MAP Kinase Kinase Kinases