An accelerated soil bait assay for the detection of potato mop top virus in agricultural soil.
An accelerated soil bait test can be used to determine whether a field harbours virus-carrying Spongospora subterranea. S. subterranea is the causal agent of powdery scab and also the only vector of potato mop top virus (PMTV). Real-time RT-PCR can detect PMTV RNA in the roots of bait plants after 2 weeks of growth in viruliferous soil. This test may be used to assess the risk of planting potato crops in a particular field.
Davey
Triona
T
DMB, Scottish Agricultural Science Agency, Edinburgh, UK.
D019411:Clinical Laboratory Techniques; D016679:Genome, Viral / Q000235:genetics; D010935:Plant Diseases / Q000821:virology*; D018517:Plant Roots / Q000821:virology; D012367:RNA, Viral / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D012987:Soil; D012988:Soil Microbiology; D011198:Solanum tuberosum / Q000821:virology*
D012367:RNA, Viral; D012987:Soil