[Detecting int I 1 gene expression of Pseudomonas aeruginosa with fluorescent quantitative PCR assay].
OBJECTIVE
In order to understand the role of integron, fluorescent quantitative polymerase chain reaction (FQ-PCR)was developed to measure the changes in int I 1 gene expression of Pseudomonas Aeruginosa in biofilm and planktonic cells.


METHODS
Three clinical strains of P. aeruginosa with int I 1 gene (SW07, R07 and TH12) were cultured in planktonic cells and biofilm cells. The total RNA of these cultured bacteria were extracted by the conventional method. The FQ-PCR was developed to measure the changes in int I 1 mRNA expression of the P. aeruginosa with bacterial 16s rRNA as an internal control.


RESULTS
The three clinical strains of P. aeruginosa expressed int I 1 mRNA in both biofilm and planktonic cells, but with different levels. The int 1 mRNA expressed by the RO7, SW07 and TH12 strains in the biofilm cells were 1.4, 5.7 and 128 times higher than in the planktonic cells, respectively.


CONCLUSIONS
The int I 1 gene expression of P. aeruginosa in the biofilm is up-regulated at mRNA level. The integron may capture and accumulate drug resistance gene cassettes more effectively in the biofilm condition.
Liu
Rong
R
Department of Microbiology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China.
Yang
Li-Li
LL
Luo
Bi-Rong
BR
Tang
Ling
L
Jiang
Hong
H
Liu
Ji-Fen
JF
Li
Ming-Yuan
MY
D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D018441:Biofilms; D015964:Gene Expression Regulation, Bacterial; D019426:Integrases / Q000235:genetics / Q000378:metabolism*; D016133:Polymerase Chain Reaction / Q000379:methods; D011550:Pseudomonas aeruginosa / Q000201:enzymology / Q000235:genetics*; D012329:RNA, Bacterial / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism
D001426:Bacterial Proteins; D012329:RNA, Bacterial; D012333:RNA, Messenger; D019426:Integrases; C115006:integron integrase IntI1