Formation of embryoid bodies from mouse embryonic stem cells cultured on silicon-coated surfaces.
Embryoid bodies (EBs) are primitive embryonic structures derived from differentiating embryonic stem cells (ESCs). Many techniques have been used to obtain EBs. Improving the technique of EB formation can help in achieving better results in ESCs differentiation into neurons, myocardiocytes, haemopoeitic cells, and others. We evaluated the use of Sigmacote as a hydrophobic substrate to improve EB formation. CCE and P19 cell lines were used to obtain EBs and retinoic acid was used to induce neural differentiation. The results revealed that Sigmacote, as a hydrophobic substrate, can improve EB formation from ESCs. Our results demonstrate that the silicon-coating of glass petri dishes by Sigmacote is an easy and reproducible technique to enhance EB formation from murine ESCs and EC cells.
Fathi
Fardin
F
Department of Anatomy, KDRC, Faculty of Medicine, Kurdistan University of Medical Sciences, Sanandaj, Iran, farfath@yahoo.com.
Altiraihi
Taki
T
Mowla
Seyed Javad
SJ
Movahedin
Mansoreh
M