Immunocapture-PCR for plant virus detection.
Immunocapture followed by the detection of viruses using polymerase chain reaction is a versatile, sensitive and robust diagnostic technique. The application of this hybrid method of virus detection in plants is particularly useful in species or tissues containing inhibitory substances. In addition, antibody-mediated virus purification is usually simpler than other methods of isolation.
Mulholland
Vincent
V
DMB, Scottish Agricultural Science Agency, Edinburgh, UK.
D019411:Clinical Laboratory Techniques; D015342:DNA Probes; D004279:DNA, Viral / Q000032:analysis; D047468:Immunoprecipitation / Q000379:methods*; D008969:Molecular Sequence Data; D010935:Plant Diseases / Q000235:genetics / Q000821:virology*; D010942:Plant Viruses / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D010944:Plants / Q000821:virology; D016133:Polymerase Chain Reaction / Q000379:methods*; D012328:RNA Viruses / Q000235:genetics / Q000302:isolation & purification; D012367:RNA, Viral / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity
D015342:DNA Probes; D004279:DNA, Viral; D012367:RNA, Viral