title: Effects of dietary restriction on mortality and age-related phenotypes in the short-lived fish Nothobranchius furzeri.
abstract: The short-lived annual fish Nothobranchius furzeri shows extremely short captive life span and accelerated expression of age markers, making it an interesting model system to investigate the effects of experimental manipulations on longevity and age-related pathologies. Here, we tested the effects of dietary restriction (DR) on mortality and age-related markers in N. furzeri. DR was induced by every other day feeding and the treatment was performed both in an inbred laboratory line and a longer-lived wild-derived line. In the inbred laboratory line, DR reduced age-related risk and prolonged maximum life span. In the wild-derived line, DR induced early mortality, did not reduce general age-related risk and caused a small but significant extension of maximum life span. Analysis of age-dependent mortality revealed that DR reduced demographic rate of aging, but increased baseline mortality in the wild-derived strain. In both inbred- and wild-derived lines, DR prevented the expression of the age markers lipofuscin in the liver and Fluoro-Jade B (neurodegeneration) in the brain. DR also improved performance in a learning test based on conditioning (active avoidance in a shuttle box). Finally, DR induced a paradoxical up-regulation of glial fibrillary acidic protein in the brain.
authors.affiliation: Biology of Aging, Fritz Lipmann Institute for Age Research, Leibniz Institute, Jena 07745 Germany.
authors.forename: Eva
authors.initials: E
authors.lastname: Terzibasi
authors.forename: Christel
authors.initials: C
authors.lastname: Lefrançois
authors.forename: Paolo
authors.initials: P
authors.lastname: Domenici
authors.forename: Nils
authors.initials: N
authors.lastname: Hartmann
authors.forename: Michael
authors.initials: M
authors.lastname: Graf
authors.forename: Alessandro
authors.initials: A
authors.lastname: Cellerino
mesh_terms: D000375:Aging / Q000502:physiology*; D000818:Animals; D015415:Biomarkers / Q000032:analysis / Q000378:metabolism; D001921:Brain / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology; D031204:Caloric Restriction / Q000379:methods*; D004396:Coloring Agents; D003213:Conditioning, Psychological / Q000502:physiology; D005399:Fishes / Q000502:physiology*; D005452:Fluoresceins; D005456:Fluorescent Dyes; D005508:Food Deprivation / Q000502:physiology*; D005904:Glial Fibrillary Acidic Protein / Q000032:analysis / Q000378:metabolism; D007178:Inbreeding; D007858:Learning / Q000502:physiology; D008062:Lipofuscin / Q000032:analysis / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D008136:Longevity / Q000235:genetics*; D009410:Nerve Degeneration / Q000209:etiology / Q000378:metabolism / Q000473:pathology; D009457:Neuroglia / Q000378:metabolism; D009930:Organic Chemicals; D010641:Phenotype
chemical_list: D015415:Biomarkers; D004396:Coloring Agents; D005452:Fluoresceins; D005456:Fluorescent Dyes; D005904:Glial Fibrillary Acidic Protein; D008062:Lipofuscin; D009930:Organic Chemicals; C435731:fluoro jade