title: Adapted liquid crystal display backlighting unit for densitometry of stained polyacrylamide electrophoresis gels.
abstract: Appropriate monochromatic illumination that is spatially uniform will ensure densitometry of stained polyacrylamide electrophoresis gels with high sensitivity and repeatability. The approach of using an array of LEDs as an illuminator has practical limitations. Here we describe an alternative method based on adapted liquid crystal display backlighting. By changing the fluorescent light source and using a region where light intensity is uniform, we demonstrate the ability to achieve highly sensitive and repeatable densitometry measurements of polyacrylamide electrophoresis gels with Coomassie-stained proteins.
authors.affiliation: Department of Mechanical & Aerospace Engineering, Monash University, VIC, Australia.
authors.forename: Han Yen
authors.initials: HY
authors.lastname: Tan
authors.forename: Tuck Wah
authors.initials: TW
authors.lastname: Ng
authors.forename: Oi Wah
authors.initials: OW
authors.lastname: Liew
mesh_terms: D003720:Densitometry / Q000295:instrumentation*; D004591:Electrophoresis, Polyacrylamide Gel; D008029:Lighting; D016014:Linear Models; D050866:Liquid Crystals; D015203:Reproducibility of Results; D012394:Rosaniline Dyes / Q000737:chemistry; D012680:Sensitivity and Specificity
chemical_list: D012394:Rosaniline Dyes; C048139:Coomassie blue