Determination of lumefantrine in rat plasma by liquid-liquid extraction using LC-MS/MS with electrospray ionization: assay development, validation and application to a pharmacokinetic study.
A simple, sensitive and rapid method for the analysis of lumefantrine in rat plasma using liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) was developed. Detection was performed by positive ion electrospray ionization (ESI) in multiple reaction monitoring (MRM) mode. The method included a chromatographic run of 5 min using a C(18) analytical column and the calibration curve was linear over the concentration range of 2-500 ng/mL with a correlation coefficient (r) of 0.996 or better. The intra- and inter-day assay precision ranged from 1.5 to 7.5% and 5.5 to 7.7%, respectively, and intra- and inter-day assay accuracy was between 91.3-109.7% and 97.0-104.7%, respectively. The method was successfully applied for the pharmacokinetic study in rats.
Wahajuddin
Pharmacokinetics and Metabolism Division, Central Drug Research Institute, Lucknow 226001, Uttar Pradesh, India.
Singh
Sheelendra Pratap
SP
Jain
Girish Kumar
GK
D000818:Animals; D000962:Antimalarials / Q000097:blood* / Q000493:pharmacokinetics; D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D004983:Ethanolamines / Q000097:blood* / Q000493:pharmacokinetics; D005449:Fluorenes / Q000097:blood* / Q000493:pharmacokinetics; D007202:Indicators and Reagents; D000078102:Lumefantrine; D008297:Male; D010616:Phenanthrenes / Q000097:blood / Q000493:pharmacokinetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D012015:Reference Standards; D015203:Reproducibility of Results; D021241:Spectrometry, Mass, Electrospray Ionization
D000962:Antimalarials; D004983:Ethanolamines; D005449:Fluorenes; D007202:Indicators and Reagents; D010616:Phenanthrenes; D000078102:Lumefantrine; C023768:halofantrine