Generation of induced pluripotent stem cells from human blood.
Human dermal fibroblasts obtained by skin biopsy can be reprogrammed directly to pluripotency by the ectopic expression of defined transcription factors. Here, we describe the derivation of induced pluripotent stem cells from CD34+ mobilized human peripheral blood cells using retroviral transduction of OCT4/SOX2/KLF4/MYC. Blood-derived human induced pluripotent stem cells are indistinguishable from human embryonic stem cells with respect to morphology, expression of surface antigens, and pluripotency-associated transcription factors, DNA methylation status at pluripotent cell-specific genes, and the capacity to differentiate in vitro and in teratomas. The ability to reprogram cells from human blood will allow the generation of patient-specific stem cells for diseases in which the disease-causing somatic mutations are restricted to cells of the hematopoietic lineage.
Loh
Yuin-Han
YH
Department of Medicine, Division of Pediatric Hematology Oncology, Children's Hospital Boston, Dana-Farber Cancer Institute, Boston, MA 02115, USA.
Agarwal
Suneet
S
Park
In-Hyun
IH
Urbach
Achia
A
Huo
Hongguang
H
Heffner
Garrett C
GC
Kim
Kitai
K
Miller
Justine D
JD
Ng
Kitwa
K
Daley
George Q
GQ
D000328:Adult; D018952:Antigens, CD34 / Q000378:metabolism; D001773:Blood Cells / Q000166:cytology* / Q000378:metabolism; D018929:Cell Culture Techniques; D054337:Cell Dedifferentiation / Q000502:physiology; D049109:Cell Proliferation; D002478:Cells, Cultured; D006801:Humans; D000090062:Kruppel-Like Factor 4; D008297:Male; D008954:Models, Biological; D039904:Pluripotent Stem Cells / Q000166:cytology* / Q000378:metabolism
D018952:Antigens, CD34; C000715467:KLF4 protein, human; D000090062:Kruppel-Like Factor 4