Identification of proteins of Neisseria meningitidis induced under iron-limiting conditions using the isobaric tandem mass tag (TMT) labeling approach.
Isobaric labeling reagents such as Tandem Mass Tags (TMT(R)) enable the genome-wide quantification of protein expression levels under different conditions using a gel-free MS/MS-based approach. Here, we applied a TMTduplex approach with two isobaric tags to study the response of the human pathogen Neisseria meningitidis to deprivation of iron, a condition met in the human body. In total, 609 proteins were identified in samples of three independent growth experiments, in which we compared cultures grown in the presence and absence of iron. Expression of 35 proteins was found to be induced or repressed under iron-limiting conditions, including 11 proteins whose ORFs were not previously identified in DNA array studies as being regulated by iron availability at the transcriptional level. These 11 proteins include proteins likely involved in iron metabolism.
van Ulsen
Peter
P
Department of Molecular Microbiology, Utrecht University, Utrecht, The Netherlands.
Kuhn
Karsten
K
Prinz
Thorsten
T
Legner
Harald
H
Schmid
Peter
P
Baumann
Christian
C
Tommassen
Jan
J
D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D020869:Gene Expression Profiling / Q000379:methods*; D015964:Gene Expression Regulation, Bacterial; D023281:Genomics; D007501:Iron / Q000378:metabolism*; D009345:Neisseria meningitidis / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D020411:Oligonucleotide Array Sequence Analysis; D015203:Reproducibility of Results; D053719:Tandem Mass Spectrometry / Q000379:methods*
D001426:Bacterial Proteins; D007501:Iron