title: Highly efficient peptide separations in proteomics. Part 2: bi- and multidimensional liquid-based separation techniques.
abstract: Multidimensional liquid-based separation techniques are described for maximizing the resolution of the enormous number of peptides generated upon tryptic digestion of proteomes, and hence, reduce the spatial and temporal complexity of the sample to a level that allows successful mass spectrometric analysis. This review complements the previous contribution on unidimensional high performance liquid chromatography (HPLC). Both chromatography and electrophoresis will be discussed albeit with reversed-phase HPLC (RPLC) as the final separation dimension prior to MS analysis.
authors.affiliation: Pronota nv, Technologiepark 4, VIB Bio-incubator, B-9052 Zwijnaarde/Ghent, Belgium. koen.sandra@richrom.com
authors.forename: Koen
authors.initials: K
authors.lastname: Sandra
authors.forename: Mahan
authors.initials: M
authors.lastname: Moshir
authors.forename: Filip
authors.initials: F
authors.lastname: D'hondt
authors.forename: Robin
authors.initials: R
authors.lastname: Tuytten
authors.forename: Katleen
authors.initials: K
authors.lastname: Verleysen
authors.forename: Koen
authors.initials: K
authors.lastname: Kas
authors.forename: Isabelle
authors.initials: I
authors.lastname: François
authors.forename: Pat
authors.initials: P
authors.lastname: Sandra
mesh_terms: D000818:Animals; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D019075:Electrophoresis, Capillary; D006801:Humans; D007525:Isoelectric Focusing; D010455:Peptides / Q000302:isolation & purification*; D040901:Proteomics / Q000379:methods*
chemical_list: D010455:Peptides