title: Effective lavage volume of diluted surfactant improves the outcome of meconium aspiration syndrome in newborn piglets.
abstract: Meconium aspiration syndrome (MAS) is one of the top causes of severe respiratory failure in neonates. This study was designed to investigate the effective volume of therapeutic bronchoalveolar lavage (BAL) with diluted surfactant in the treatment of MAS in newborn piglets. Human meconium was instilled in 24 piglets to induce MAS, and the piglets were randomly divided into four groups: 1) control, no lavage; 2) lavage-10, BAL with diluted surfactant (5 mg/mL, Survanta) 10 mL/kg in two aliquots; 3) lavage-20, 20 mL/kg in two aliquots; 4) lavage-30, 30 mL/kg in two aliquots. Cardiopulmonary parameters were monitored, and the lung tissue was histologically examined after experiments. The changes in oxygenation and lung compliance of lavage-20 and lavage-30 groups were significantly better than control and lavage-10 groups (p < 0.05), but there was no significant difference between lavage-20 and lavage-30 groups. The lung injury scores were significantly lower in the dependent site of lavage-20 and lavage-30 groups than the other two groups. In conclusion, using 20 mL/kg diluted surfactant in two aliquots to perform therapeutic BAL was as effective as 30 mL/kg in improving the pathophysiological outcomes in MAS and may warrant consideration clinically in treating MAS.
authors.affiliation: Institute of Emergency and Critical Care Medicine, Department of Pediatrics, National Yang-Ming University, Taipei, Taiwan, R.O.C. mjjeng@vghtpe.gov.tw
authors.forename: Mei-Jy
authors.initials: MJ
authors.lastname: Jeng
authors.forename: Wen-Jue
authors.initials: WJ
authors.lastname: Soong
authors.forename: Yu-Sheng
authors.initials: YS
authors.lastname: Lee
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D001688:Biological Products / Q000494:pharmacology / Q000627:therapeutic use*; D001794:Blood Pressure; D001831:Body Temperature; D018893:Bronchoalveolar Lavage / Q000379:methods*; D004562:Electrocardiography; D006801:Humans; D007231:Infant, Newborn; D008170:Lung Compliance / Q000187:drug effects; D008471:Meconium Aspiration Syndrome / Q000628:therapy*; D011663:Pulmonary Surfactants / Q000494:pharmacology / Q000627:therapeutic use*; D034421:Sus scrofa
chemical_list: D001688:Biological Products; D011663:Pulmonary Surfactants; C072197:beractant