Zn stress-induced inhibition of bean PvSR2-GUS fusion gene splicing is gene-specific in transgenic tobacco.
The stress-related gene no. 2 of Phaseolus vulgaris (PvSR2) is metal inducible and contains a single intron. Here, we report that Zn stress inhibited the splicing of the PvSR2-beta-glucuronidase (GUS) fusion gene in a concentration- and time-dependent manner in tobacco seedlings. The inhibition appears to be specific for the PvSR2-GUS transgene: splicing of four endogenous tobacco genes was unaffected by Zn stress. Our results provide in vivo evidence that Zn stress-dependent intron retention is transgene specific in plants.
Qi
Xiaoting
X
College of Life Science, Capital Normal University, Beijing 100048, China. qixiaoting@mail.cnu.edu.cn
Cui
Qiuhong
Q
Luo
Ying
Y
Guo
Chunfen
C
Chai
Tuanyao
T
D018506:Gene Expression Regulation, Plant / Q000187:drug effects / Q000235:genetics; D050939:Gene Fusion / Q000235:genetics*; D007438:Introns / Q000235:genetics; D027805:Phaseolus / Q000235:genetics*; D030821:Plants, Genetically Modified / Q000187:drug effects* / Q000235:genetics*; D012326:RNA Splicing / Q000187:drug effects*; D014026:Nicotiana / Q000187:drug effects* / Q000235:genetics*; D015032:Zinc / Q000494:pharmacology*
D015032:Zinc