[Pancreatic islets isolation from the brain death donors].
The paper analyzes a number of 5 cases of pancreatic islet cells isolation, purification and culture extracted from the pancreas of brain-dead donors. For the isolation stage the authors used the "two-step" digestion method. The isolated pancreatic islet cells were morphologically and functionally analyzed by assessing their viability and purity. For the in-vivo experiments the authors used animals with Streptozotocin induced diabetes. The data clearly indicated a high efficiency of collection providing an optimal number of transplantable pancreatic islet cells.
Dima
Simona
S
Universitatea "Ovidius" Constanţa, Facultatea de Medicină, Clinica a II-a Chirurgicală.
Sârbu
V
V
Balaban
Doina
D
Botea
F
F
Pasăre
Raluca
R
D000818:Animals; D001926:Brain Death; D002102:Cadaver; D018929:Cell Culture Techniques / Q000379:methods*; D002469:Cell Separation / Q000379:methods; D003921:Diabetes Mellitus, Experimental / Q000601:surgery; D003922:Diabetes Mellitus, Type 1 / Q000601:surgery; D004195:Disease Models, Animal; D006801:Humans; D007515:Islets of Langerhans; D016381:Islets of Langerhans Transplantation / Q000379:methods; D051379:Mice; D014019:Tissue Donors; D014183:Transplantation, Heterologous; D016896:Treatment Outcome