Gold immunochromatographic assay for simultaneous detection of carbofuran and triazophos in water samples.
Using a simple test for rapid identification and quantification of pesticide multiresidues in food and environmental samples is a long-cherished approach for practical monitoring purposes. Here two gold-based lateral-flow strips (strip A and strip B) were investigated for simultaneous detection of carbofuran and triazophos. For the strip A format, a bispecific monoclonal antibody (BsMcAb) against both carbofuran and triazophos was employed to prepare the immunogold probe. For the strip B format, anti-carbofuran monoclonal antibody (McAb) and anti-triazophos McAb separately labeled with colloidal gold were combined as detector reagents. By comparison of visual results from pesticide standard tests between the two formats, the strip B assay manifested higher sensitivities for both pesticides. Analysis of spiked water samples by the preferable strip indicated that the detection limits for carbofuran and triazophos were 32 and 4 microg/L, respectively. The strength of the portable one-step strip assay was in the simultaneous screening for two pesticides within a short time (8-10 min) without any equipment.
Guo
Yi-Rong
YR
Institute of Pesticide and Environmental Toxicology, Zhejiang University, Hangzhou 310029, China.
Liu
Shao-Ying
SY
Gui
Wen-Jun
WJ
Zhu
Guo-Nian
GN
D000911:Antibodies, Monoclonal / Q000276:immunology*; D002235:Carbofuran / Q000032:analysis* / Q000276:immunology; D007150:Immunohistochemistry / Q000379:methods*; D063086:Organothiophosphates / Q000032:analysis* / Q000276:immunology; D010575:Pesticides / Q000032:analysis* / Q000276:immunology; D014230:Triazoles / Q000032:analysis* / Q000276:immunology; D014867:Water / Q000032:analysis*; D014874:Water Pollutants, Chemical / Q000032:analysis* / Q000276:immunology
D000911:Antibodies, Monoclonal; D063086:Organothiophosphates; D010575:Pesticides; D014230:Triazoles; D014874:Water Pollutants, Chemical; D014867:Water; C012584:triazophos; D002235:Carbofuran