A solution for stripping antibodies from polyvinylidene fluoride immunoblots for multiple reprobing.
Available protocols for stripping antibodies from immunoblots involve the use of sodium dodecyl sulfate (SDS) or low-pH buffers. SDS was shown to remove transferred proteins from membranes, and low-pH buffer was shown to inefficiently strip off antibodies. A solution containing 6M guanidine hydrochloride, 0.2% nondenaturing detergent, and a reducing agent can rapidly strip off tightly bound antibodies from aged polyvinylidene fluoride (PVDF) immunoblots at room temperature without removing significant amounts of transferred protein.
Yeung
Yee-Guide
YG
Department of Developmental and Molecular Biology, Albert Einstein College of Medicine of Yeshiva University, Bronx, NY 10461, USA.
Stanley
E Richard
ER
D000906:Antibodies / Q000737:chemistry*; D003902:Detergents / Q000737:chemistry; D019791:Guanidine / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D015151:Immunoblotting; D011145:Polyvinyls / Q000737:chemistry*; D013696:Temperature; D005465:Fluorocarbon Polymers
D000906:Antibodies; D003902:Detergents; D019791:Guanidine; D011145:Polyvinyls; C024865:polyvinylidene fluoride; D005465:Fluorocarbon Polymers