Mouse androgenetic embryonic stem cells differentiated to multiple cell lineages in three embryonic germ layers in vitro.
The embryos of some rodents and primates can precede early development without the process of fertilization; however, they cease to develop after implantation because of restricted expressions of imprinting genes. Asexually developed embryos are classified into parthenote/gynogenote and androgenote by their genomic origins. Embryonic stem cells (ESCs) derived from asexual origins have also been reported. To date, ESCs derived from parthenogenetic embryos (PgESCs) have been established in some species, including humans, and the possibility to be alternative sources for autologous cell transplantation in regenerative medicine has been proposed. However, some developmental characteristics, which might be important for therapeutic applications, such as multiple differentiation capacity and transplantability of the ESCs of androgenetic origin (AgESCs) are uncertain. Here, we induced differentiation of mouse AgESCs and observed derivation of neural cells, cardiomyocytes and hepatocytes in vitro. Following differentiated embryoid body (EB) transplantation in various mouse strains including the strain of origin, we found that the EBs could engraft in theoretically MHC-matched strains. Our results indicate that AgESCs possess at least two important characteristics, multiple differentiation properties in vitro and transplantability after differentiation, and suggest that they can also serve as a source of histocompatible tissues for transplantation.
Teramura
Takeshi
T
Department of Obstetrics and Gynecology, Graduate School of Medicine, Mie University. teramura@med.kindai.ac.jp
Onodera
Yuta
Y
Murakami
Hideki
H
Ito
Syunsuke
S
Mihara
Toshihiro
T
Takehara
Toshiyuki
T
Kato
Hiromi
H
Mitani
Tasuku
T
Anzai
Masayuki
M
Matsumoto
Kazuya
K
Saeki
Kazuhiro
K
Fukuda
Kanji
K
Sagawa
Norimasa
N
Osoi
Yoshihiko
Y
D000818:Animals; D002454:Cell Differentiation / Q000502:physiology*; D019070:Cell Lineage / Q000502:physiology*; D002478:Cells, Cultured; D004622:Embryo, Mammalian; D053595:Embryonic Stem Cells / Q000502:physiology*; D005260:Female; D005855:Germ Layers / Q000502:physiology*; D022781:Hepatocytes / Q000276:immunology / Q000502:physiology / Q000637:transplantation; D007108:Immune Tolerance / Q000502:physiology; D051379:Mice; D008810:Mice, Inbred C57BL; D032383:Myocytes, Cardiac / Q000276:immunology / Q000502:physiology / Q000637:transplantation; D009474:Neurons / Q000276:immunology / Q000502:physiology / Q000637:transplantation; D010312:Parthenogenesis / Q000502:physiology; D011247:Pregnancy