title: Stimulation of cell motility and expression of late markers of differentiation in human oral keratinocytes by Candida albicans.
abstract: A hallmark of the mucosa of immunocompromized hosts in oral candidiasis is a hyperkeratinized region heavily colonized with fungi at the surface of the terminally differentiated epithelium. To gain insight into the processes important for promoting mucosal invasion by fungi, we characterized the response of keratinocytes to the presence of Candida albicans. Indirect immunofluorescence and kymographic analyses revealed a multifaceted keratinocyte response of OKF6/TERT-2 cells to C. albicans that consisted of: cytoskeletal reorganization within 3 h, motility and cell expansion with formation of E-cadherin-mediated cell-cell adhesions within 6 h, increased expression of late differentiation markers and decreased expression of calprotectin. The initial expansive phase was followed by dissolution of cell-cell adhesions and a decrease in cell size accompanied by loss of E-cadherin. The keratinocyte response depended on soluble factors associated with hyphal growth as demonstrated using the efg1Delta/efg1Delta, cap1Delta/cap1Delta, als3Delta/als3Delta, hwp1Delta/hwp1Deltaand sap4-6Delta/sap4-6Delta mutants and was not observed in the presence of the non-pathogenic yeast, Saccharomyces cerevisiae. These studies show the potential for C. albicans to manipulate the stratified epithelial cells to a state of differentiation that is more permissive of fungal colonization of oral tissue, which is likely to play an important role in the pathogenesis of candidiasis.
authors.affiliation: Microbiology and Molecular Pathogenesis Program, Dartmouth Medical School, Hanover, NH 03755, USA.
authors.forename: Christiane
authors.initials: C
authors.lastname: Rollenhagen
authors.forename: Torsten
authors.initials: T
authors.lastname: Wöllert
authors.forename: George M
authors.initials: GM
authors.lastname: Langford
authors.forename: Paula
authors.initials: P
authors.lastname: Sundstrom
mesh_terms: D015820:Cadherins / Q000378:metabolism; D002176:Candida albicans / Q000502:physiology*; D002448:Cell Adhesion; D002465:Cell Movement; D002478:Cells, Cultured; D018920:Coculture Techniques; D003599:Cytoskeleton / Q000378:metabolism; D005656:Fungal Proteins / Q000235:genetics / Q000502:physiology; D017353:Gene Deletion; D005786:Gene Expression Regulation; D054884:Host-Pathogen Interactions; D006801:Humans; D015603:Keratinocytes / Q000382:microbiology* / Q000502:physiology*; D037521:Virulence Factors / Q000235:genetics / Q000502:physiology
chemical_list: D015820:Cadherins; D005656:Fungal Proteins; D037521:Virulence Factors