Involvement of PLC-beta3 in the effect of morphine on memory retrieval in passive avoidance task.
Phospholipase C (PLC) is one signalling effector enzyme whose activity is directly modulated by opioids. Several physiological studies have implicated PLC-linked pathways in in-vivo pain regulation and opioid tolerance. Co-administration of PLC-beta(2/3) activity blocker M119 with morphine resulted in a dramatic increase in morphine-induced amnesic effect in mice, proving a role for beta subunit of PLC enzyme in these processes. Administration of morphine to mice at amnesic dose increased PLC-beta(3) activity, with respect to basal value, in the membrane-soluble material from anterior cortex and hippocampal formation in brain areas. PLC-beta(3) appears to be simultaneously implicated in both analgesic and amnesic effects induced by administration of morphine to mice suggesting a commonality in the molecular mechanisms of morphine-induced analgesia and memory impairment.
Bianchi
E
E
Department of Neuroscience, University of Siena, Siena, Italy. bianchi16@unisi.it
Lehmann
D
D
Vivoli
E
E
Norcini
M
M
Ghelardini
C
C
D000704:Analysis of Variance; D000818:Animals; D001362:Avoidance Learning / Q000187:drug effects* / Q000502:physiology; D002540:Cerebral Cortex / Q000187:drug effects* / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D006624:Hippocampus / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D011939:Mental Recall / Q000187:drug effects* / Q000502:physiology; D051379:Mice; D009020:Morphine / Q000494:pharmacology*; D009294:Narcotics / Q000494:pharmacology; D054799:Phospholipase C beta / Q000378:metabolism*; D017450:Receptors, Opioid, mu / Q000378:metabolism
D009294:Narcotics; D017450:Receptors, Opioid, mu; D009020:Morphine; D054799:Phospholipase C beta