Hibiscus sabdariffa inhibits vascular smooth muscle cell proliferation and migration induced by high glucose--a mechanism involves connective tissue growth factor signals.
Recently, the herbal extract of Hibiscus sabdariffa was shown to have multiple bioactive effects, including anti-atherosclerosis. On the basis of this, we aimed to examine whether the polyphenolic isolate of H. sabdariffa (HPI) could protect high-glucose-treated vascular smooth muscle cell (VSMC) and its putative transduction signals. Results showed that HPI dose- and time-dependently reduced the high-glucose-stimulated cell proliferation and migration. HPI suppressed the proliferating cell nuclear antigen (PCNA) level and matrix metalloproteinase (MMP)-2 activation. In addition, the expressions of connective tissue growth factor (CTGF) and receptor of advanced glycation end product (RAGE) enhanced by high glucose were prominently suppressed by HPI. The proliferation signal mediated by high glucose was demonstrated via CTGF/RAGE, while MMP-2 was regulated by CTGF but not RAGE. Conclusively, the results suggest that HPI potentially can be a promising adjuvant herbal therapy for diabetic patients.
Huang
Chien-Ning
CN
Department of Internal Medicine, Chung-Shan Medical University Hospital, School of Medicine, Chung-Shan Medical University, Taichung 402, Taiwan.
Chan
Kuei-Chuan
KC
Lin
Wei-Ting
WT
Su
Shi-Li
SL
Wang
Chau-Jong
CJ
Peng
Chiung-Huei
CH
D000818:Animals; D001011:Aorta; D002455:Cell Division / Q000187:drug effects*; D002465:Cell Movement / Q000187:drug effects*; D002478:Cells, Cultured; D055513:Connective Tissue Growth Factor / Q000032:analysis / Q000235:genetics / Q000502:physiology*; D004789:Enzyme Activation / Q000187:drug effects; D005419:Flavonoids / Q000302:isolation & purification / Q000494:pharmacology*; D005947:Glucose / Q000494:pharmacology; D031584:Hibiscus / Q000737:chemistry*; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism; D009131:Muscle, Smooth, Vascular / Q000737:chemistry / Q000166:cytology*; D010636:Phenols / Q000302:isolation & purification / Q000494:pharmacology*; D059808:Polyphenols; D018809:Proliferating Cell Nuclear Antigen / Q000032:analysis; D034741:RNA, Small Interfering / Q000235:genetics; D051381:Rats; D000067759:Receptor for Advanced Glycation End Products; D011971:Receptors, Immunologic / Q000032:analysis / Q000502:physiology; D015398:Signal Transduction / Q000187:drug effects; D014162:Transfection
D005419:Flavonoids; D010636:Phenols; D059808:Polyphenols; D018809:Proliferating Cell Nuclear Antigen; D034741:RNA, Small Interfering; D000067759:Receptor for Advanced Glycation End Products; D011971:Receptors, Immunologic; D055513:Connective Tissue Growth Factor; D020778:Matrix Metalloproteinase 2; D005947:Glucose