[Analysis of differential protein expression of REMI mutant of Trichoderma koningii].
On purpose of expounding the phenotype change of Trichoderma mutant which produces cellulase, protein profiles of T. koningii CICC and its mutant TK-2R-1 were detected by 2-DE. Eight unique spots were found and four of them were identified and their functions by MS-TOF-TOF were connected with growth and metabolization of Trichoderma spp. The research provides a new theoretical basis and methods for the construction of Trichoderma mutant which is used to produce efficient cellulase.
Hu
Shi Feng
SF
Hunan Agriculture University, Changsha 410128, China.
Zhu
Mu Lan
ML
Liu
Li Xing
LX
Tang
Jun
J
Gao
Bi Da
BD
Chen
Jie
J
D002480:Cellulase / Q000378:metabolism; D015180:Electrophoresis, Gel, Two-Dimensional; D005656:Fungal Proteins / Q000032:analysis* / Q000378:metabolism; D005786:Gene Expression Regulation; D013058:Mass Spectrometry; D009154:Mutation / Q000235:genetics; D010641:Phenotype; D014242:Trichoderma / Q000235:genetics* / Q000378:metabolism*
D005656:Fungal Proteins; D002480:Cellulase