title: Expression and localization of regenerating gene I in a rat liver regeneration model.
abstract: Regenerating gene (Reg) I has been identified as a regenerative/proliferative factor for pancreatic islet cells. We examined Reg I expression in the regenerating liver of a rat model that had been administered 2-acetylaminofluorene and treated with 70% partial hepatectomy (2-AAF/PH model), where hepatocyte and cholangiocyte proliferation was suppressed and the hepatic stem cells and/or hepatic progenitor cells were activated. In a detailed time course study of activation of hepatic stem cells in the 2-AAF/PH model, utilizing immunofluorescence staining with antibodies of Reg I and other cell-type-specific markers, we found that Reg I-expressing cells are present in the bile ductules and increased during regeneration. Reg I-expressing cells were colocalized with CK19, OV6, and AFP. These results demonstrate that Reg I is significantly upregulated in the liver of the 2-AAF/PH rat model, accompanied by the formation of bile ductules during liver regeneration.
authors.affiliation: Department of Biochemistry, Akita University School of Medicine, 1-1-1 Hondo, Akita 010-8543, Japan.
authors.forename: Jingshu
authors.initials: J
authors.lastname: Wang
authors.forename: Souichi
authors.initials: S
authors.lastname: Koyota
authors.forename: Xiaoping
authors.initials: X
authors.lastname: Zhou
authors.forename: Yasuharu
authors.initials: Y
authors.lastname: Ueno
authors.forename: Li
authors.initials: L
authors.lastname: Ma
authors.forename: Masami
authors.initials: M
authors.lastname: Kawagoe
authors.forename: Yukio
authors.initials: Y
authors.lastname: Koizumi
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Okamoto
authors.forename: Toshihiro
authors.initials: T
authors.lastname: Sugiyama
mesh_terms: D000818:Animals; D001652:Bile Ducts / Q000378:metabolism; D015870:Gene Expression; D051847:Lithostathine / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D008099:Liver / Q000378:metabolism*; D008115:Liver Regeneration; D008297:Male; D023421:Models, Animal; D051381:Rats; D011916:Rats, Inbred F344; D015854:Up-Regulation
chemical_list: D051847:Lithostathine; C497616:Reg1a protein, rat