The rabies virus phosphoprotein synthesis and subcellular localization are modified by nerve growth factor.
Rabies virus P protein participates as a regulating factor in viral transcription and replication; recent studies found an antitranscriptional and antireplicative effect of nerve growth factor (NGF) and Neurotrophin-3 (NT-3) in infected neuron cultures. We investigated here the specific effect of the neurotrophins on P protein, evaluating its synthesis and subcellular distribution in adult mouse dorsal root ganglia neuron cultures infected and treated with NGF or NT-3. The results showed that NGF, but not NT-3, caused an increase in the quantity of P protein and an accumulation of protein in neuronal bodies, revealing changes in transport to the neuritic processes.
Castaneda
Nadia Y
NY
Instituto de Virologia, Universidad El Bosque, Bogota, Colombia.
Chaparro-Olaya
Jacqueline
J
Acosta
Orlando
O
Castellanos
Jaime E
JE
D000818:Animals; D002478:Cells, Cultured; D004797:Enzyme-Linked Immunosorbent Assay; D005455:Fluorescent Antibody Technique; D005470:Fluorometry; D005727:Ganglia, Spinal / Q000378:metabolism / Q000821:virology*; D051379:Mice; D008813:Mice, Inbred ICR; D018832:Molecular Chaperones; D020932:Nerve Growth Factor / Q000378:metabolism*; D020933:Neurotrophin 3 / Q000378:metabolism*; D010750:Phosphoproteins / Q000378:metabolism*; D011820:Rabies virus / Q000502:physiology*; D011984:Sensory Receptor Cells / Q000378:metabolism* / Q000821:virology*; D015678:Viral Structural Proteins / Q000378:metabolism*
D018832:Molecular Chaperones; D020933:Neurotrophin 3; C103961:P phosphoprotein, Rabies virus; D010750:Phosphoproteins; D015678:Viral Structural Proteins; D020932:Nerve Growth Factor