title: Optimizing the performance of tin dioxide microspheres for phosphopeptide enrichment.
abstract: Phosphopeptide enrichment based on metal oxide affinity chromatography is one of the most powerful tools for studying protein phosphorylation on a large scale. To complement existing metal oxide sorbents, we have recently introduced tin dioxide as a promising alternative. The preparation of SnO(2) microspheres by the nanocasting technique, using silica of different morphology as a template, offers a strategy to prepare materials that vary in their particle size and their porosity. Here, we demonstrate how such stannia materials can be successfully generated and their properties fine-tuned in order to obtain an optimized phosphopeptide enrichment material. We combined data from liquid chromatography-mass spectrometry experiments and physicochemical characterization, including nitrogen physisorption and energy-dispersive X-ray spectroscopy (EDX), to explain the influence of the various experimental parameters.
authors.affiliation: Department of Analytical Chemistry and Food Chemistry, University of Vienna, Waehringer Strasse 38, 1090 Vienna, Austria. alexander.leitner@univie.ac.at
authors.forename: Alexander
authors.initials: A
authors.lastname: Leitner
authors.forename: Martin
authors.initials: M
authors.lastname: Sturm
authors.forename: Jan-Henrik
authors.initials: JH
authors.lastname: Smått
authors.forename: Mikael
authors.initials: M
authors.lastname: Järn
authors.forename: Mika
authors.initials: M
authors.lastname: Lindén
authors.forename: Karl
authors.initials: K
authors.lastname: Mechtler
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Lindner
mesh_terms: D000595:Amino Acid Sequence; D002846:Chromatography, Affinity; D002853:Chromatography, Liquid; D013058:Mass Spectrometry; D008863:Microspheres; D036103:Nanotechnology; D010316:Particle Size; D010748:Phosphopeptides / Q000032:analysis* / Q000302:isolation & purification; D010766:Phosphorylation; D016062:Porosity; D017971:Tin Compounds / Q000737:chemistry*
chemical_list: D010748:Phosphopeptides; D017971:Tin Compounds; C045358:stannic oxide