Interactions between chronic stress and chronic consumption of caffeine on the enzymatic antioxidant system.
We studied the effect of chronic caffeine on parameters related to oxidative stress in different brain regions of stressed and non-stressed rats. Wistar rats were divided into three groups: control (receiving water), caffeine 0.3 g/L and caffeine 1.0 g/L (in the drinking water). These groups were subdivided into non-stressed and stressed (repeated restraint stress during 40 days). Lipid peroxide levels and the total radical-trapping potential were assessed, as well as antioxidant enzyme activities superoxide dismutase, gluthatione peroxidase, and catalase in hippocampus, striatum and cerebral cortex. Results showed interactions between stress and caffeine, especially in the cerebral cortex, since caffeine increased the activity of some antioxidant enzymes, but not in stressed animals. We concluded that chronic administration of caffeine led, in some cases, to increased activity of antioxidant enzymes. However, these effects were not observed in the stressed animals.
Noschang
Cristie Grazziotin
CG
Departamento de Bioquímica, Instituto de Ciências Básica da Saúde, ICBS, UFRGS, Ramiro Barcellos, Porto Alegre, RS, Brazil. cristienoschang@hotmail.com
Krolow
Rachel
R
Pettenuzzo
Leticia Ferreira
LF
Avila
Mônica Colpini
MC
Fachin
Andrelisa
A
Arcego
Danusa
D
von Pozzer Toigo
Eduardo
E
Crema
Leonardo Machado
LM
Diehl
Luísa Amália
LA
Vendite
Deusa
D
Dalmaz
Carla
C
D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D002110:Caffeine / Q000494:pharmacology*; D002374:Catalase / Q000378:metabolism; D002540:Cerebral Cortex / Q000378:metabolism; D003342:Corpus Striatum / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000378:metabolism; D006624:Hippocampus / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar; D012149:Restraint, Physical; D013315:Stress, Psychological / Q000378:metabolism*; D013482:Superoxide Dismutase / Q000378:metabolism; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
D000975:Antioxidants; D017392:Thiobarbituric Acid Reactive Substances; D002110:Caffeine; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase