Cytogenetic investigation of chromosomal aberrations in cells treated with plantamajoside from Plantago asiatica.
Plantago asiatica is a member of the Plantaginaceae family, and is widely distributed in East Asia. In our previous work, a single active compound, plantamajoside was isolated and confirmed to have glycation inhibitory activity, and did not possess toxicity during a 90 day repeated oral toxicity test in rats. In the present study, a chromosomal aberration test was performed to investigate the genotoxicity of plantamajoside. From the results of the cytotoxicity test, plantamajoside proved to be less toxic when it was treated combined with S9 cell fractions. However, there was a significant increase in structural aberrations during the short-term treatment of plantamajoside at its highest dose (5000 microg/mL) even when combined with S9. This seems to have been a natural phenomenon due to the very high dose of plantamajoside that was used. However, to confirm the safety of plantamajoside for its potential use as a phytochemical agent in health products, additional mutagenicity tests are necessary.
Koo
Yun-Chang
YC
Division of Food Bioscience and Technology, College of Life Sciences and Biotechnology, Korea University, Seoul, Republic of Korea.
Jung
Sung-Hoon
SH
Yang
Ji-Hee
JH
Ryu
Yung-Sun
YS
Kim
Eun-Jin
EJ
Lee
Kwang-Won
KW
D000818:Animals; D001711:Biotransformation / Q000235:genetics; D002396:Catechols / Q000302:isolation & purification / Q000633:toxicity*; D002460:Cell Line; D002869:Chromosome Aberrations; D002875:Chromosomes / Q000187:drug effects*; D006224:Cricetinae; D003412:Cricetulus; D004305:Dose-Response Relationship, Drug; D005347:Fibroblasts / Q000187:drug effects; D005960:Glucosides / Q000302:isolation & purification / Q000633:toxicity*; D010936:Plant Extracts / Q000737:chemistry / Q000633:toxicity*; D010943:Plantago / Q000737:chemistry*
D002396:Catechols; D005960:Glucosides; D010936:Plant Extracts; C421173:plantamajoside