title: Preparation of immunogens and production of antibodies.
abstract: The quality of reagents greatly affects the interpretation of serological tests. Methods used in conventional viral purification and molecular cloning and expression of target viral proteins to obtain antigens for immunization are presented. Immunization of rabbits, mice and chickens and isolation of immunoglobulin from immunized animals also are described.
authors.affiliation: Floral and Nursery Plants Research Unit, U.S. National Arboretum, USDA-ARS, Beltsville, MD 20705, USA.
authors.forename: Hei-Ti
authors.initials: HT
authors.lastname: Hsu
authors.forename: Tsung-Chi
authors.initials: TC
authors.lastname: Chen
authors.forename: Chin-An
authors.initials: CA
authors.lastname: Chang
authors.forename: Shyi-Dong
authors.initials: SD
authors.lastname: Yeh
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D000917:Antibody Formation / Q000276:immunology* / Q000502:physiology; D000918:Antibody Specificity / Q000502:physiology*; D000941:Antigens / Q000276:immunology / Q000302:isolation & purification*; D019411:Clinical Laboratory Techniques; D007114:Immunization / Q000379:methods*; D051379:Mice; D011817:Rabbits; D012680:Sensitivity and Specificity; D014614:Vaccines, Synthetic / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D000941:Antigens; D014614:Vaccines, Synthetic