title: Visualization of plastids in pollen grains: involvement of FtsZ1 in pollen plastid division.
abstract: Visualizing organelles in living cells is a powerful method to analyze their intrinsic mechanisms. Easy observation of chlorophyll facilitates the study of the underlying mechanisms in chloroplasts, but not in other plastid types. Here, we constructed a transgenic plant enabling visualization of plastids in pollen grains. Combination of a plastid-targeted fluorescent protein with a pollen-specific promoter allowed us to observe the precise number, size and morphology of plastids in pollen grains of the wild type and the ftsZ1 mutant, whose responsible gene plays a central role in chloroplast division. The transgenic material presented in this work is useful for studying the division mechanism of pollen plastids.
authors.affiliation: Research Institute for Bioresources, Okayama University, Kurashiki, Okayama 710-0046, Japan.
authors.forename: Lay Yin
authors.initials: LY
authors.lastname: Tang
authors.forename: Noriko
authors.initials: N
authors.lastname: Nagata
authors.forename: Ryo
authors.initials: R
authors.lastname: Matsushima
authors.forename: Yuling
authors.initials: Y
authors.lastname: Chen
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Yoshioka
authors.forename: Wataru
authors.initials: W
authors.lastname: Sakamoto
mesh_terms: D017360:Arabidopsis / Q000166:cytology / Q000235:genetics* / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D008854:Microscopy, Electron; D008856:Microscopy, Fluorescence; D016254:Mutagenesis, Insertional; D030821:Plants, Genetically Modified / Q000235:genetics / Q000378:metabolism; D018087:Plastids / Q000235:genetics / Q000378:metabolism / Q000648:ultrastructure*; D011058:Pollen / Q000166:cytology* / Q000378:metabolism
chemical_list: D029681:Arabidopsis Proteins; C523306:FtsZ1 protein, Arabidopsis; D049452:Green Fluorescent Proteins