title: Involvement of matrix metalloproteinases on the inhibition of cells invasion and migration by emodin in human neuroblastoma SH-SY5Y cells.
abstract: Emodin (1,3,8-trihydroxy-6-methylanthaquinone), an active component present in the root and rhizome of Rheum palmatum L. (Polygonaceae) has anti-bacterial, anti-tumor, diuretic and vasorelaxant effects. However, its mechanism of action on the cell migration and invasion of human neuroblastoma cancer SH-SY5Y cells is not fully understood. In this study, firstly, the effects of emodin on the percentage of viable cells were examined by using MTT assay and it was found that emodin induced dose-and time-dependent inhibition in human neuroblastoma SH-SY5Y cells. Second, the effects of emodin on the migration and invasion of SH-SY5Y cells were examined by using wound assay and matrigel counting and the results showed that emodin suppressed the migration and invasion of SH-SY5Y cells. Third, we examined the effect of emodin on the levels of associated proteins by using Western blotting and the results indicated that emodin inhibited the levels of GRB2, RhoA, HIF-1alpha, VEGF, FAK, iNOS, COX2, p-p38, p-c-jun, MMP2, MMP9 and MMP7 but promoted the levels of PKC, PI3K, MEKK3 and NF-kappaB p65 that led to the inhibition of migration and invasion of SH-SY5Y cells in vitro.
authors.affiliation: Department of Clinical Pathology, Cheng Hsin Rehabilitation Medical Center, Taipei, Taiwan, ROC.
authors.forename: Hsu-Feng
authors.initials: HF
authors.lastname: Lu
authors.forename: Kuang-Chi
authors.initials: KC
authors.lastname: Lai
authors.forename: Shu-Chun
authors.initials: SC
authors.lastname: Hsu
authors.forename: Hui-Ju
authors.initials: HJ
authors.lastname: Lin
authors.forename: Chao-Lin
authors.initials: CL
authors.lastname: Kuo
authors.forename: Ching-Lung
authors.initials: CL
authors.lastname: Liao
authors.forename: Jai-Sing
authors.initials: JS
authors.lastname: Yang
authors.forename: Jing-Gung
authors.initials: JG
authors.lastname: Chung
mesh_terms: D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects; D004642:Emodin / Q000494:pharmacology*; D051417:Focal Adhesion Kinase 1 / Q000378:metabolism; D006801:Humans; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism; D020783:Matrix Metalloproteinase 7 / Q000378:metabolism; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism; D020782:Matrix Metalloproteinases / Q000502:physiology*; D009361:Neoplasm Invasiveness / Q000517:prevention & control; D009447:Neuroblastoma / Q000473:pathology*; D014945:Wound Healing / Q000187:drug effects
chemical_list: D051417:Focal Adhesion Kinase 1; C496050:PTK2 protein, human; D020782:Matrix Metalloproteinases; D020783:Matrix Metalloproteinase 7; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9; D004642:Emodin