Imaging neural stem cell fate in mouse model of glioma.
This unit describes a protocol for following the fate of stem cells in real time in a mouse model of glioma. Stem cells and tumor cells can be transduced with lentiviral vectors bearing two different luciferases, firefly luciferase (Fluc) and Renilla (Rluc) luciferase, respectively. With the cells labeled in this manner, bioluminescence imaging can be used to study the fate of stem cells in glioma-bearing brains in vivo.
Shah
Khalid
K
Massachusetts General Hospital, Harvard Medical School, Charlestown, Massachusetts, USA.
D000818:Animals; D003952:Diagnostic Imaging / Q000379:methods*; D004195:Disease Models, Animal; D005822:Genetic Vectors; D005910:Glioma / Q000473:pathology*; D016086:Lentivirus / Q000235:genetics; D049409:Luciferases, Firefly; D049410:Luciferases, Renilla; D008163:Luminescent Measurements; D008722:Methods; D051379:Mice; D009474:Neurons / Q000473:pathology*; D013234:Stem Cells / Q000473:pathology*; D014161:Transduction, Genetic
D049410:Luciferases, Renilla; D049409:Luciferases, Firefly