Signaling pathways associated with the expression of inflammatory mediators activated during the course of two models of experimental periodontitis.
OBJECTIVE
Evaluate the signaling pathways associated with inflammatory mediators activated in two models of experimental periodontitis.


METHODS
Two models were used: lipopolysaccharide (LPS) injections and ligature placement. Wistar rats were used and 30 microg LPS from Escherichia coli was injected twice a week into the palatal aspect of the upper molars. Ligatures were placed around lower first molars. A control group received injections of PBS on the palatal gingivae whereas no ligatures were placed on the lower molars. Samples were collected 5, 15 and 30 days and processed for analysis by Western blotting and stereometry.


RESULTS
The ligature model was associated with rapid and transient activation of extracellular-regulated kinases (ERK) and p38 mitogen-activated protein kinase (MAPK) as well as of nuclear factor kappa B (NF-kappaB). Activation of these signaling pathways on the LPS model was delayed but sustained throughout the 30-day experimental period. Inflammatory changes induced by both models were similar; however there was a significant reduction on inflammation degree on the ligature model, which paralleled the decrease observed on the activation of the signaling pathways. Activation of signal transducer and activator of transcription (STAT)-3 by phosphorylation of Tyrosine residues and of STAT-5 was observed only on the ligature model.


CONCLUSIONS
Regulation of gene expression results from the activation of signaling pathways initiated by receptor-ligand binding of external antigens and also of cytokines produced by the host immune system. Understanding the signaling pathways relevant for a given condition may provide information useful for novel therapeutic approaches.
Garcia de Aquino
Sabrina
S
Department of Diagnosis and Surgery, School of Dentistry at Araraquara, State University of Sao Paulo, Rua Humaita, 1680-Centro, Araraquara, SP 14801-903, Brazil.
Manzolli Leite
Fabio Renato
FR
Stach-Machado
Dagmar Ruth
DR
Francisco da Silva
Juliete Aparecida
JA
Spolidorio
Luís Carlos
LC
Rossa
Carlos
C
D000818:Animals; D015153:Blotting, Western; D048049:Extracellular Signal-Regulated MAP Kinases / Q000502:physiology; D005881:Gingiva / Q000378:metabolism / Q000473:pathology; D007150:Immunohistochemistry; D018836:Inflammation Mediators / Q000378:metabolism*; D008026:Ligation; D008070:Lipopolysaccharides / Q000494:pharmacology; D008297:Male; D016328:NF-kappa B / Q000096:biosynthesis / Q000235:genetics; D010518:Periodontitis / Q000378:metabolism* / Q000503:physiopathology*; D010766:Phosphorylation; D051381:Rats; D017208:Rats, Wistar; D050799:STAT5 Transcription Factor / Q000096:biosynthesis / Q000235:genetics; D015398:Signal Transduction / Q000502:physiology*; D014443:Tyrosine / Q000378:metabolism; D048051:p38 Mitogen-Activated Protein Kinases / Q000502:physiology
D018836:Inflammation Mediators; D008070:Lipopolysaccharides; D016328:NF-kappa B; D050799:STAT5 Transcription Factor; D014443:Tyrosine; D048049:Extracellular Signal-Regulated MAP Kinases; D048051:p38 Mitogen-Activated Protein Kinases