Microfluidic stickers for cell- and tissue-based assays in microchannels.
Difficulties in culturing cells inside microchannels is a major obstacle for the wide use of microfluidic technology in cell biology. Here, we present a simple and versatile method to interface closed microchannels with cellular and multicellular systems. Our approach, based on microfluidic stickers which can adhere to wet glass coverslips, eliminates the need to adapt cell culture conditions to microchannels and greatly facilitates the methods required to position cells into microcircuits. We demonstrate the simplicity and efficiency of the method with HeLa cells, primary cultured neurons and Drosophila tissues.
Morel
Mathieu
M
Laboratoire Kastler Brossel, CNRS UMR8552, Physics and Biology Department, Ecole Normale Supérieure and Université Pierre et Marie Curie-Paris 6, France.
Bartolo
Denis
D
Galas
Jean-Christophe
JC
Dahan
Maxime
M
Studer
Vincent
V
D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry; D002448:Cell Adhesion; D018929:Cell Culture Techniques / Q000295:instrumentation / Q000379:methods; D002470:Cell Survival; D002478:Cells, Cultured; D004330:Drosophila / Q000166:cytology; D004867:Equipment Design; D005456:Fluorescent Dyes / Q000378:metabolism; D005727:Ganglia, Spinal / Q000166:cytology; D006367:HeLa Cells; D006801:Humans; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation* / Q000379:methods; D044085:Microfluidics / Q000295:instrumentation* / Q000379:methods; D008846:Micromanipulation / Q000295:instrumentation / Q000379:methods; D009474:Neurons / Q000166:cytology*; D012235:Rhodamines / Q000378:metabolism; D013379:Substrate Specificity
D001672:Biocompatible Materials; D005456:Fluorescent Dyes; D012235:Rhodamines; C029773:rhodamine B