[Construction and evaluation of a genetic engineered strain for biodesulfurization].
We first cloned the dsz operon of Pseudomonas delafieldii R-8 into the expressing plasmid (pPR9TT) to construct the recombinant plasmid pPR-dsz, and then reintroduced it into strain R-8 to obtain a muti-copy dsz operon engineering strain R-8-1. Compared with the wild-type, strain R-8-1 showed a higher desulfurization activity for dibenzothiophene (DBT). Initial rates of DBT removal by strain R-8-1 were 6.25 micromol/g dry cell/h, about 2-fold higher than that for wild-type strain. The recombinant cells were also applied in the desulfurization of diesel. It resulted in a 68% reduction of total sulfur from 310.8 mg/L to 100.1 mg/L, whereas only 53% of sulfur was removed by strain R-8. The stability of pPR-dsz in strain R-8-1 was studied. The results revealed the first obtain a muti-copy dsz operon engineering strain are helpful for further development in biodesulfurization.
Li
Huanjie
H
Laboratory of Biochemical and Molecular Biology, Graduate School of Chinese Academy of Agricultural Sciences, Beijing 100081, China.
Yu
Zhijian
Z
Xiong
Xiaochao
X
Li
Yuguang
Y
Li
Xin
X
D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D001673:Biodegradation, Environmental; D005818:Genetic Engineering; D024861:Genetic Enhancement / Q000379:methods*; D009876:Operon; D010105:Oxygenases / Q000235:genetics; D015202:Protein Engineering / Q000379:methods*; D011549:Pseudomonas / Q000235:genetics* / Q000378:metabolism; D013455:Sulfur / Q000378:metabolism*; D013876:Thiophenes / Q000378:metabolism*
D001426:Bacterial Proteins; D013876:Thiophenes; D013455:Sulfur; D010105:Oxygenases; C421210:dibenzothiophene sulfone monooxygenase; C016366:dibenzothiophene