Inflammatory and oxidative parameters in cord blood as diagnostic of early-onset neonatal sepsis: a case-control study.
OBJECTIVE
To determine whether levels of interleukin (IL)-6, IL-10, and oxidative parameters in umbilical cord blood could contribute as an indicator of neonatal sepsis in recognized high-risk neonates.


METHODS
Prospective, case-control study.


METHODS
Neonatal intensive care unit.


METHODS
One hundred twenty consecutive preterm neonates who had at least one other risk factor for early-onset neonatal sepsis.


METHODS
None.


RESULTS
Umbilical cord blood samples were obtained for the determination of IL-6, IL-10, thiobarbituric acid reactive substances (TBARS), and protein carbonyls levels. Neonates were divided prospectively in two groups: control and septic. All parameters were higher in septic patients compared with control (IL-6 184.6 +/- 72.7 vs. 58.9 +/- 19.1, p < 0.01; IL-10 171.4 +/- 59.2 vs. 79.9 +/- 17.9, p < 0.01; TBARS 10.1 +/- 2.8 vs. 4.2 +/- 2.5, p < 0.01; protein carbonyls 2.4 +/- 1.2 vs. 1.15 +/- 0.5, p < 0.01, respectively, septic vs. control). In addition, these parameters were higher in the subgroup of culture-positive septic patients compared with control. IL-6 and TBARS had equivalent areas under the receiver operator characteristic (ROC) curve (0.88); IL-10 (0.80) and protein carbonyls (0.73) had lower areas. Multivariate logistic regression comparing IL-6 and TBARS in terms of the relative risk for neonatal sepsis demonstrated that TBARS was a better predictor, being independently associated with neonatal sepsis.


CONCLUSIONS
Our findings demonstrated that cord blood IL-6, IL-10, and oxidative stress markers were significantly higher in infants with neonatal sepsis, and only TBARS levels were independently related to the development of neonatal sepsis in our sample.
Cancelier
Ana Carolina
AC
Laboratório de Fisiopatologia Experimental, Programa de Pós-Graduação em Ciências da Saúde, Unidade Acadêmica de Ciências da Saúde, Universidade do Extremo Sul Catarinense, Criciuma, SC, Brazil.
Petronilho
Fabricia
F
Reinke
Adalisa
A
Constantino
Larissa
L
Machado
Roberta
R
Ritter
Cristiane
C
Dal-Pizzol
Felipe
F
D015415:Biomarkers / Q000097:blood; D016022:Case-Control Studies; D005260:Female; D005312:Fetal Blood / Q000737:chemistry*; D006801:Humans; D007231:Infant, Newborn; D007234:Infant, Premature; D007249:Inflammation / Q000097:blood; D007363:Intensive Care Units, Neonatal; D016753:Interleukin-10 / Q000097:blood*; D015850:Interleukin-6 / Q000097:blood*; D008297:Male; D018384:Oxidative Stress; D011446:Prospective Studies; D050050:Protein Carbonylation; D012372:ROC Curve; D012307:Risk Factors; D018805:Sepsis / Q000097:blood* / Q000175:diagnosis* / Q000382:microbiology; D013292:Streptococcus agalactiae / Q000302:isolation & purification; D017392:Thiobarbituric Acid Reactive Substances / Q000032:analysis
D015415:Biomarkers; D015850:Interleukin-6; D017392:Thiobarbituric Acid Reactive Substances; D016753:Interleukin-10