The efficacy of alpha-tocopherol in functional recovery of spinal cord injured rats: an experimental study.
OBJECTIVE
The objective of this study is to examine the effects of the alpha-tocopherol on rats with spinal cord injury (SCI).


METHODS
Research Center, Sultan Bin Abdulaziz Humanitarian City, Riyadh, Kingdom of Saudi Arabia.


METHODS
Female Sprague-Dawley rats weighing 180-220 g were anesthetized with chloral hydrate (450 mg kg(-1) body weight) by intraperitoneal injection and laminectomy was performed at the T 7-8 level leaving the dura intact. A compression plate (2.2 x 5.0 mm) was loaded with a weight of 35 g placed on the exposed cord for 5 min to create SCI. The subjects were divided into three groups of eight rats each. Group 1 served as control (SCI+saline); whereas groups 2 and 3 served as test groups, alpha-tocopherol was given orally in doses of 1000 mg kg(-1) body weight for group 2 and 2000 mg kg(-1) body weight for group 3, respectively. Daily activities were recorded in the activity cage for 14 days post-operatively.


RESULTS
At day 1 (baseline, 24 h after the surgery), there was no significant difference between mean motor scores of all groups. After day 1, the three groups showed continuous improvement in motor score; such improvement was maintained throughout the duration of the study with different levels for each group. By the end of the study (day 14), groups 2 and 3 showed statistically significant improvement in the mean motor score compared with group 1 (P<0.05). However, no significant difference was observed between test groups 2 and 3 by the end of the study.


CONCLUSIONS
The results suggest that the administration of alpha-tocopherol may have reparative effects for SCI because of its antioxidant effect.
Al Jadid
M S I
MS
Medical Affairs, Sultan Bin Abdulaziz Humanitarian City, Riyadh, Saudi Arabia.
Robert
A
A
Al-Mubarak
S
S
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology* / Q000627:therapeutic use*; D001522:Behavior, Animal / Q000187:drug effects; D001835:Body Weight / Q000187:drug effects; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D005260:Female; D009068:Movement / Q000187:drug effects; D051381:Rats; D017207:Rats, Sprague-Dawley; D020127:Recovery of Function / Q000187:drug effects*; D013119:Spinal Cord Injuries / Q000188:drug therapy* / Q000503:physiopathology*; D013997:Time Factors; D024502:alpha-Tocopherol / Q000494:pharmacology* / Q000627:therapeutic use*
D000975:Antioxidants; D024502:alpha-Tocopherol