Regulation of tyrosine phosphatases in the adventitia during vascular remodelling.
Protein tyrosine phosphatases (PTPs) are regulators of growth factor signalling in vascular remodelling. The aim of this study was to evaluate PTP expression in the context of PDGF-signalling in the adventitia after angioplasty. Utilising a rat carotid artery model, the adventitial layers of injured and non-injured vessels were laser microdissected. The mRNA expression of the PDGF beta-receptor, the ligands PDGF-A/B/C/D and the receptor-antagonising PTPs (DEP-1, TC-PTP, SHP-2, PTP1B) were determined and correlated to vascular morphometrics, proliferation markers and PDGF beta-receptor phosphorylation. The levels of the PDGF beta-receptor, PDGF-C and PDGF-D were upregulated concurrently with the antagonising PTPs DEP-1 and TC-PTP at day 8, and normalised at day 14 after vessel injury. Although the proliferation parameters were time-dependently altered in the adventitial layer, the phosphorylation of the PDGF beta-receptor remained unchanged. The expression dynamics of specific PTPs indicate a regulatory role of PDGF-signalling also in the adventitia during vascular remodelling.
Micke
Patrick
P
Cancer Centrum Karolinska, Department of Oncology-Pathology, Karolinska Institute, Stockholm, Sweden.
Hackbusch
Daniel
D
Mercan
Sibel
S
Stawowy
Philipp
P
Tsuprykov
Oleg
O
Unger
Thomas
T
Ostman
Arne
A
Kappert
Kai
K
D000818:Animals; D002339:Carotid Arteries / Q000378:metabolism / Q000503:physiopathology*; D020212:Carotid Artery Injuries / Q000378:metabolism / Q000503:physiopathology*; D049109:Cell Proliferation; D003238:Connective Tissue / Q000201:enzymology*; D004730:Endothelium, Vascular / Q000378:metabolism / Q000503:physiopathology; D020869:Gene Expression Profiling; D023421:Models, Animal; D010766:Phosphorylation; D010982:Platelet-Derived Growth Factor / Q000096:biosynthesis; D017027:Protein Tyrosine Phosphatases / Q000096:biosynthesis*; D051381:Rats; D017207:Rats, Sprague-Dawley; D020797:Receptor, Platelet-Derived Growth Factor beta / Q000096:biosynthesis / Q000378:metabolism*; D012038:Regeneration; D015398:Signal Transduction
D010982:Platelet-Derived Growth Factor; D020797:Receptor, Platelet-Derived Growth Factor beta; D017027:Protein Tyrosine Phosphatases