title: The effect of the co-immobilization of human osteoprogenitors and endothelial cells within alginate microspheres on mineralization in a bone defect.
abstract: Bone regeneration seems to be dependant on cell communication between osteogenic and endothelial cells arising from surrounding blood vessels. This study aims to determine whether endothelial cells can regulate the osteogenic potential of osteoprogenitor cells in vitro and in vivo, in a long bone defect, when co-immobilized in alginate microspheres. Alginate is a natural polymer widely used as a biomaterial for cell encapsulation. Human osteoprogenitors (HOP) from bone marrow mesenchymal stem cells were immobilized alone or together with human umbilical vein endothelial cells (HUVEC) inside irradiated, oxidized and RGD-grafted alginate microspheres. Immobilized cells were cultured in dynamic conditions and cell metabolic activity increased during three weeks. The gene expression of alkaline phosphatase and osteocalcin, both specific markers of the osteoblastic phenotype, and mineralization deposits were upregulated in co-immobilized HOPs and HUVECs, comparing to the immobilization of monocultures. VEGF secretion was also increased when HOPs were co-immobilized with HUVECs. Microspheres containing co-cultures were further implanted in a bone defect and bone formation was analysed by muCT and histology at 3 and 6 weeks post-implantation. Mineralization was observed inside and around the implanted microspheres containing the immobilized cells. However, when HOPs were co-immobilized with HUVECs, mineralization significantly increased. These findings demonstrate that co-immobilization of osteogenic and endothelial cells within RGD-grafted alginate microspheres provides a promising strategy for bone tissue engineering.
authors.affiliation: INSERM, U577, Bordeaux and Université Victor Segalen Bordeaux 2, UMR-S577, F-33076 Bordeaux Cedex, France. maritie.grellier@inserm.fr
authors.forename: Maritie
authors.initials: M
authors.lastname: Grellier
authors.forename: Pedro L
authors.initials: PL
authors.lastname: Granja
authors.forename: Jean-Christophe
authors.initials: JC
authors.lastname: Fricain
authors.forename: Sílvia J
authors.initials: SJ
authors.lastname: Bidarra
authors.forename: Martine
authors.initials: M
authors.lastname: Renard
authors.forename: Reine
authors.initials: R
authors.lastname: Bareille
authors.forename: Chantal
authors.initials: C
authors.lastname: Bourget
authors.forename: Joelle
authors.initials: J
authors.lastname: Amédée
authors.forename: Mário A
authors.initials: MA
authors.lastname: Barbosa
mesh_terms: D000328:Adult; D000368:Aged; D000464:Alginates / Q000737:chemistry / Q000378:metabolism; D000818:Animals; D001861:Bone Regeneration / Q000502:physiology*; D001842:Bone and Bones / Q000378:metabolism / Q000473:pathology*; D002113:Calcification, Physiologic; D002478:Cells, Cultured; D042783:Endothelial Cells / Q000166:cytology / Q000502:physiology*; D020723:Glucuronic Acid / Q000737:chemistry / Q000378:metabolism; D006603:Hexuronic Acids / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D008863:Microspheres; D008875:Middle Aged; D010641:Phenotype; D033581:Stem Cell Transplantation; D013234:Stem Cells / Q000166:cytology / Q000502:physiology*; D055815:Young Adult
chemical_list: D000464:Alginates; D006603:Hexuronic Acids; D020723:Glucuronic Acid