title: Optically modulated fluorophores for selective fluorescence signal recovery.
abstract: Fluorescence imaging in biological sciences is hindered by significant depth-dependent signal attenuation and highly fluorescent backgrounds. We have developed optically modulated near-IR-emitting few-atom Ag nanodots that are selectively and dynamically photobrightened upon simultaneous excitation with a secondary laser, enabling high-sensitivity image extraction to reveal only the demodulated fluorophores. Image demodulation is demonstrated in high-background environments to extract weak signals from completely obscuring background emission.
authors.affiliation: School of Chemistry and Biochemistry and Petit Institute for Biosciences and Bioengineering, Georgia Institute of Technology, Atlanta, Georgia 30332-0400, USA.
authors.forename: Chris I
authors.initials: CI
authors.lastname: Richards
authors.forename: Jung-Cheng
authors.initials: JC
authors.lastname: Hsiang
authors.forename: Dulal
authors.initials: D
authors.lastname: Senapati
authors.forename: Sandeep
authors.initials: S
authors.lastname: Patel
authors.forename: Junhua
authors.initials: J
authors.lastname: Yu
authors.forename: Tom
authors.initials: T
authors.lastname: Vosch
authors.forename: Robert M
authors.initials: RM
authors.lastname: Dickson
mesh_terms: D000818:Animals; D002232:Carbocyanines / Q000737:chemistry*; D003584:Cytological Techniques; D003952:Diagnostic Imaging; D005453:Fluorescence; D006046:Gold / Q000737:chemistry*; D051379:Mice; D041681:NIH 3T3 Cells; D043942:Nanotubes / Q000737:chemistry*; D055095:Optics and Photonics; D010777:Photochemistry
chemical_list: D002232:Carbocyanines; C085321:cyanine dye 5; D006046:Gold