Preventive effect of pentoxifylline on acute radiation damage via antioxidant and anti-inflammatory pathways.
OBJECTIVE
The aim of the present study was to investigate whether pentoxifylline (PTX) treatment could protect against induced acute radiation enteritis.


METHODS
Rats received 100 mg/kg/day PTX for 7 days before irradiation and continued on treatment for 3 days after irradiation. The intestinal myeloperoxidase (MPO) activities and malondialdehyde (MDA), glutathione (GSH), prostaglandin E2, and thromboxane B2 levels were determined. Terminal ileum tissue was evaluated for morphological changes. Also, nuclear factor kappa (NF-kappa), tumor necrosis factor-alpha (TNF-alpha), and intercellular adhesion molecule 1 (ICAM-1) expressions were analyzed with immunohistochemisty methods.


RESULTS
PTX treatment was associated with increased GSH levels and decreased MPO activity and MDA, prostaglandin E2, and thromboxane B2 levels. Histopathologic examination showed that intestinal mucosal structure was preserved in the PTX-treated group while having significant decreases in NF-kappaB, TNF-a, and ICAM-1 expression.


CONCLUSIONS
PTX appears to have a protective effect against radiation damage. This protective effect is mediated in part by decreasing both inflammatory reactions and oxidative stress.
Hepgül
Gülçin
G
Istanbul Medical Faculty, Department of General Surgery, Istanbul University, Istanbul, Turkey.
Tanrikulu
Sevda
S
Unalp
Haluk Recai
HR
Akguner
Taner
T
Erbil
Yeşim
Y
Olgaç
Vakur
V
Ademoğlu
Evin
E
D000208:Acute Disease; D000818:Animals; D015232:Dinoprostone / Q000032:analysis; D004751:Enteritis / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*; D005978:Glutathione / Q000032:analysis; D007082:Ileum / Q000473:pathology; D007150:Immunohistochemistry; D018799:Intercellular Adhesion Molecule-1 / Q000032:analysis; D008297:Male; D008315:Malondialdehyde / Q000032:analysis; D016328:NF-kappa B / Q000032:analysis; D010431:Pentoxifylline / Q000627:therapeutic use*; D009195:Peroxidase / Q000378:metabolism; D011833:Radiation Injuries, Experimental / Q000517:prevention & control*; D011837:Radiation-Protective Agents / Q000627:therapeutic use*; D051381:Rats; D017208:Rats, Wistar; D013929:Thromboxane B2 / Q000032:analysis; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis
D016328:NF-kappa B; D011837:Radiation-Protective Agents; D014409:Tumor Necrosis Factor-alpha; D018799:Intercellular Adhesion Molecule-1; D008315:Malondialdehyde; D013929:Thromboxane B2; D009195:Peroxidase; D005978:Glutathione; D015232:Dinoprostone; D010431:Pentoxifylline