Up-regulated expression of peroxisome proliferator-activated receptor gamma in the hypothalamic-pituitary-adrenal axis of weaned pigs after Escherichia coli lipopolysaccharide challenge.
The expression of peroxisome proliferator-activated receptor gamma (PPARgamma) was investigated in the hypothalamic-pituitary-adrenal (HPA) axis of weaned pigs after injection with 100 microg/kg bodyweight Escherichia coli lipopolysaccharide (LPS) (n=6) and control pigs injected with sterile saline (n=6). LPS increased PPARgamma mRNA and protein expression in the hypothalamus (23.8 and 3.1-fold relative to controls, respectively), pituitary gland (9.2 and 2.0-fold, respectively) and adrenal gland (3.5 and 2.3-fold, respectively) (P<0.05). LPS also induced an increase in PPARgamma immunohistochemical staining in the hypothalamus (1.3-fold), adenohypophysis (1.3-fold), adrenal cortex (1.4-fold) and adrenal medulla (1.6-fold) (P<0.05). Concurrent with up-regulated expression of PPARgamma, LPS increased the concentrations of plasma corticotrophin-releasing hormone (2.1-fold) and adrenocorticotrophin (1.4-fold) (P<0.05). LPS also induced elevations of interleukin 6 and tumour necrosis factor alpha mRNA levels in the hypothalamus (4.0 and 3.2-fold, respectively), pituitary gland (20.7 and 5.1-fold, respectively) and adrenal gland (3.9 and 3.3-fold, respectively) (P<0.05). PPARgamma may play a role in the regulation of neuroendocrine responses associated with immunological stress in pigs.
Liu
Yu Lan
YL
Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan 430023, China. yulanflower@126.com
Shi
Jun Xia
JX
Lu
Jing
J
Che
Zheng Quan
ZQ
Zhu
Hui Ling
HL
Hou
Yong Qing
YQ
Yin
Yu Long
YL
Zhao
Sheng Jun
SJ
Ding
Bin Ying
BY
Liu
Hong Ming
HM
D000818:Animals; D015153:Blotting, Western / Q000662:veterinary; D004926:Escherichia coli; D007030:Hypothalamo-Hypophyseal System / Q000187:drug effects* / Q000502:physiology; D007150:Immunohistochemistry / Q000662:veterinary; D008070:Lipopolysaccharides / Q000494:pharmacology*; D047495:PPAR gamma / Q000187:drug effects* / Q000378:metabolism*; D010913:Pituitary-Adrenal System / Q000187:drug effects* / Q000502:physiology; D011897:Random Allocation; D013552:Swine; D015854:Up-Regulation; D014886:Weaning
D008070:Lipopolysaccharides; D047495:PPAR gamma