Aroclor 1254 impairs spermatogenesis and induces oxidative stress in rat testicular mitochondria.
Aroclor 1254 (A1254) has been shown to have potential testicular toxicity. The mechanism of action of A1254 on male reproduction is not clear. The present study was designed to investigate the potential toxicity of A1254 on rat spermatogenesis. Oxidative stress was also assessed in testicular mitochondria as an underlying mechanism. Adult male Wistar rats were injected with A1254 (0, 0.75, 1.5 or 3mg/kg/day i.p.) or with vehicle (corn oil) for 20 consecutive days. A1254 at doses of 1.5 and 3mg/kg/day resulted in a significant decrease in body weight, testes weight, epididymal and relative epididymal weight. Similarly, the relative testis weight was significantly decreased at 3mg/kg/day. Sperm count, motility and daily sperm production were significantly decreased at 1.5 and 3mg/kg/day. The same two doses significantly inhibited the activities of testicular mitochondrial CAT, GPx and GR while the activity of SOD was significantly decreased by 0.75, 1.5 and 3mg/kg/day. The levels of H(2)O(2) generation and LPO were significantly increased in mitochondria in a dose-related pattern. GSH and Vit C were significantly decreased at 0.75, 1.5 and 3mg/kg/day. In conclusion, A1254 impairs spermatogenesis as evidenced, at least partly, by induction of oxidative stress in testicular mitochondria.
Aly
Hamdy A A
HA
Department of Pharmacology and Toxicology, Faculty of Pharmacy, Al-Azhar University, Nasr City, Cairo, Egypt. hamdyaali@yahoo.com
Domènech
Oscar
O
Abdel-Naim
Ashraf B
AB
D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D001205:Ascorbic Acid / Q000378:metabolism; D001835:Body Weight / Q000187:drug effects; D002374:Catalase / Q000378:metabolism; D020111:Chlorodiphenyl (54% Chlorine) / Q000633:toxicity*; D005978:Glutathione / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000378:metabolism; D005980:Glutathione Reductase / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism*; D009929:Organ Size / Q000187:drug effects; D018384:Oxidative Stress / Q000187:drug effects*; D051381:Rats; D017208:Rats, Wistar; D013076:Sperm Count; D013081:Sperm Motility / Q000187:drug effects; D013091:Spermatogenesis / Q000187:drug effects*; D013482:Superoxide Dismutase / Q000378:metabolism; D013737:Testis / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology
D000975:Antioxidants; D020111:Chlorodiphenyl (54% Chlorine); D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D005980:Glutathione Reductase; D005978:Glutathione; D001205:Ascorbic Acid