Kaempferol isolated from Nelumbo nucifera stamens negatively regulates FcepsilonRI expression in human basophilic KU812F cells.
Mast cells and basophils perform important functions as pivotal effector cells in IgE-mediated allergic reactions. KU812F cells, a human basophilic cell line isolated originally from chronic myelocytic leukemia, express a high affinity receptor of IgE, FcepsilonRI. Kaempferol was extracted and isolated from a methanolic extract of flavonoid-rich Nelumbo nucifera stamens. In the present study, the inhibitory effects of kaempferol on FcepsilonRI expression in human basophilic KU812F cells was examined. Flow cytometric analysis revealed that FcepsilonRI expression on the cell surface was suppressed in a concentration-dependent manner when the cells were cultured with kaempferol. Moreover, RTPCR analysis showed that the mRNA levels for FcepsilonRI alpha- and gamma-chains were reduced as the result of kaempferol treatment in a concentration-dependent manner. Kaempferol showed its suppressive effects on intracellular Ca2+ concentration and histamine release from anti-FcepsilonRI alpha- chain antibody-stimulated cells in a concentration-dependent manner. These observations indicate that kaempferol may exert antiallergic effect via downregulation of FcepsilonRI expression and degranulation.
Shim
Sun-Yup
SY
Institute of Marine Life Science, Pukyong National University, Busan 608-737, Korea.
Choi
Jae-Sue
JS
Byun
Dae-Seok
DS
D001491:Basophils / Q000187:drug effects*; D002118:Calcium / Q000378:metabolism; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D015870:Gene Expression; D006636:Histamine Release / Q000187:drug effects; D006801:Humans; D044949:Kaempferols / Q000494:pharmacology*; D015394:Molecular Structure; D031653:Nelumbo / Q000737:chemistry*; D010936:Plant Extracts / Q000494:pharmacology; D017455:Receptors, IgE / Q000378:metabolism*
D044949:Kaempferols; D010936:Plant Extracts; D017455:Receptors, IgE; C006552:kaempferol; D002118:Calcium