Detection of Bacillus sphaericus mosquitocidal toxin genes by multiplex colony PCR.
A multiplex colony PCR assay was developed for the detection of 5 genes encoding Bacillus sphaericus mosquito larvicidal toxins, namely binA, binB, mtx1, mtx2, and mtx3. Primers designed for these 5 genes yielded specific PCR amplicons of the expected size from type cultures of B. sphaericus. This method of detecting multiple toxin genes by colony PCR in a single tube reaction is a simple, rapid, and economical technique for identification of highly toxic environmental B. sphaericus isolates.
Jagtap
Santosh C
SC
Department of Marine Biotechnology, Ministry of Defence, Anandnagar, Ambernath, Mumbai-421506, India.
Jagtap
Chandrakant B
CB
Kumar
Pradeep
P
Srivastava
R B
RB
D001407:Bacillus / Q000235:genetics*; D001427:Bacterial Toxins / Q000235:genetics*; D017931:DNA Primers / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods*
D001427:Bacterial Toxins; D017931:DNA Primers; C046134:binB protein, Bacillus sphaericus