The expression and prognostic significance of Cks1 in salivary cancer.
Cks1 is an essential factor in facilitating Skp2-dependent degradation of p27, but its role in salivary malignancies is unknown. Expression of cyclin-dependent kinase subunit 1 (Cks1) was examined in 64 salivary malignancies, compared with p27, S-phase kinase protein 2 (Skp2), Ki-67, p53, and TDT-mediated dutp-biotin nick end labeling (TUNEL) expression, and with THE patient's clinical and pathological parameters. Cks1 expression was markedly increased in 30 patients (47%) and strongly correlated with increased expression of Skp2, Ki-67, p53, and TUNEL, but inversely with p27 levels. High expression of Cks1 WAS strongly associated with lymph node metastases and poor prognosis and survival. Cks1 alterations may have a significant biological role in the pathogenesis of salivary cancer.
Nagler
Rafael M
RM
Department of Oral and Maxillofacial Surgery and Oral Biochemistry Laboratory, Rambam Medical Center and Bruce Rappaport Faculty of Medicine, Technion-Israel Institute of Technology, Haifa, Israel. nagler@tx.technion.ac.il
Ben-Izhak
Ofer
O
Ostrovsky
Dimitri
D
Golz
Avishai
A
Hershko
Dan D
DD
D000230:Adenocarcinoma / Q000201:enzymology / Q000556:secondary; D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000378:metabolism; D042846:CDC2-CDC28 Kinases; D018267:Carcinoma, Acinar Cell / Q000201:enzymology / Q000556:secondary; D018277:Carcinoma, Mucoepidermoid / Q000201:enzymology / Q000556:secondary; D002294:Carcinoma, Squamous Cell / Q000201:enzymology / Q000556:secondary; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D018844:Cyclin-Dependent Kinases / Q000235:genetics / Q000378:metabolism*; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D020287:In Situ Nick-End Labeling; D019394:Ki-67 Antigen / Q000378:metabolism; D008207:Lymphatic Metastasis; D008297:Male; D008875:Middle Aged; D011379:Prognosis; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism; D044786:S-Phase Kinase-Associated Proteins / Q000378:metabolism; D012468:Salivary Gland Neoplasms / Q000201:enzymology* / Q000473:pathology; D015996:Survival Rate; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism; D055815:Young Adult
D014408:Biomarkers, Tumor; C093090:CKS1B protein, human; D002352:Carrier Proteins; D019394:Ki-67 Antigen; D018809:Proliferating Cell Nuclear Antigen; D044786:S-Phase Kinase-Associated Proteins; D016159:Tumor Suppressor Protein p53; C413374:p27 antigen; D042846:CDC2-CDC28 Kinases; D018844:Cyclin-Dependent Kinases