title: 4-Methoxycinnamaldehyde inhibited human respiratory syncytial virus in a human larynx carcinoma cell line.
abstract: 4-Methoxycinnamaldehyde, an active constituent of Agastache rugosa, was examined for its cytoprotective activity against RSV by XTT method in human larynx carcinoma cell line. 4-Methoxycinnamaldehyde could effectively inhibit cytopathic effect of RSV (p<0.0001) with an estimated IC(50) of 0.055microg/ml and a selectivity index (SI) of 898.2. 4-Methoxycinnamaldehyde (0.03microg/ml) could inhibit viral entrance by interfering viral attachment (IC(50) of 0.06microg/ml; p<0.0001) and internalization (IC(50) of 0.01microg/ml; p<0.0001). 4-Methoxycinnamaldehyde significantly increased the basal production of IFN (p=0.0015), but not the virus-induced IFN production. Therefore, its cytoprotective activity against RSV was not mediated by interferon. In conclusion, 4-methoxycinnamaldehyde might be helpful to manage the disease induced by RSV infection.
authors.affiliation: Graduate Institute of Natural Products, College of Pharmacy, Kaohsiung Medical University, Kaohsiung, Taiwan.
authors.forename: Kuo Chih
authors.initials: KC
authors.lastname: Wang
authors.forename: Jung San
authors.initials: JS
authors.lastname: Chang
authors.forename: Lien Chai
authors.initials: LC
authors.lastname: Chiang
authors.forename: Chun Ching
authors.initials: CC
authors.lastname: Lin
mesh_terms: D000171:Acrolein / Q000031:analogs & derivatives* / Q000494:pharmacology / Q000627:therapeutic use; D031327:Agastache / Q000737:chemistry*; D045744:Cell Line, Tumor; D006801:Humans; D007372:Interferons / Q000378:metabolism; D007822:Laryngeal Neoplasms; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology / Q000627:therapeutic use*; D018357:Respiratory Syncytial Virus Infections / Q000150:complications / Q000188:drug therapy* / Q000821:virology; D018113:Respiratory Syncytial Virus, Human / Q000187:drug effects*; D053585:Virus Attachment / Q000187:drug effects; D053586:Virus Internalization / Q000187:drug effects
chemical_list: C488838:4-methoxycinnamaldehyde; D010936:Plant Extracts; D000171:Acrolein; D007372:Interferons