Novel U-bent fiber optic probe for localized surface plasmon resonance based biosensor.
The aim of this study is to develop an optical absorbance based biosensor suitable for wide scale use in resource-poor locales. A sensor for sensitive measurement of refractive index (RI) with the help of optical absorbance properties of gold nanoparticles (GNP) coupled to an efficient optical transducer in the form of a U-bent fiber optic probe is described. A U-bent probe was fabricated by a simple procedure. The absorbance due to the localized surface plasmon resonance (LSPR) of fiber-bound GNP was found to be linear to refractive index changes between 1.33 and 1.35. A U-bent probe of 200 microm diameter with a bend radius of 0.75 mm gave rise to a sensitivity of 35 DeltaA/RIU at 540 nm. The resolution of the sensor probe was 3.8x10(-5) RIU. Label-free biosensing was demonstrated using these probes with the help of IgG-anti IgG as bioreceptor-analyte pair.
Sai
V V R
VV
School of Biosciences and Bioengineering, IIT Bombay, Mumbai 400076, India.
Kundu
Tapanendu
T
Mukherji
Soumyo
S
D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D006046:Gold / Q000737:chemistry*; D006801:Humans; D055767:Immobilized Proteins / Q000032:analysis / Q000276:immunology; D007118:Immunoassay / Q000295:instrumentation / Q000379:methods; D007074:Immunoglobulin G / Q000032:analysis / Q000276:immunology; D053768:Metal Nanoparticles / Q000737:chemistry*; D055100:Optical Fibers; D012031:Refractometry / Q000295:instrumentation / Q000379:methods*; D012680:Sensitivity and Specificity; D013053:Spectrophotometry; D020349:Surface Plasmon Resonance / Q000379:methods*
D055767:Immobilized Proteins; D007074:Immunoglobulin G; D006046:Gold