title: Biomolecular oxidative damage activated by enzymatic logic systems: biologically inspired approach.
abstract: Systems that perform oxidative damage to biomolecules through catalytic cascades in the presence of iron-redox labile species were activated by enzymatic logic gates that process chemical input signals according to built-in logic operations. AND/OR enzymatic logic gates were composed of glucose oxidase (GOx) and GOx/esterase, respectively. The AND/OR logic functions of the enzyme gates were activated by application of glucose-oxygen and glucose-ethyl acetate input signals, respectively. The enzymatic logic gates, upon activation by specific patterns of the chemical input signals, produced acidic solutions and triggered release of redox labile iron species from a complex that is unstable under acidic conditions. This resulted in the activation of a catalytic cascade, which produced reactive oxygen species (ROS) and subsequently yielded oxidative damage in biomolecules. Functional integration of the enzyme-based logic systems with the catalytic redox cascade that performs damage in biomolecules on demand is a first step towards "smart" systems capable of programmed detection, identification, and neutralization of potential biohazards.
authors.affiliation: Department of Chemistry and Biomolecular Science and NanoBio Laboratory, Clarkson University, Potsdam, NY 13699-5810, USA.
authors.forename: Jian
authors.initials: J
authors.lastname: Zhou
authors.forename: Galina
authors.initials: G
authors.lastname: Melman
authors.forename: Marcos
authors.initials: M
authors.lastname: Pita
authors.forename: Maryna
authors.initials: M
authors.lastname: Ornatska
authors.forename: Xuemei
authors.initials: X
authors.lastname: Wang
authors.forename: Artem
authors.initials: A
authors.lastname: Melman
authors.forename: Evgeny
authors.initials: E
authors.lastname: Katz
mesh_terms: D000042:Absorption; D055162:Biocatalysis; D032701:Biomimetics; D004798:Enzymes / Q000378:metabolism*; D005949:Glucose Oxidase / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D008128:Logic; D008954:Models, Biological; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D017382:Reactive Oxygen Species / Q000378:metabolism
chemical_list: D004798:Enzymes; D017382:Reactive Oxygen Species; D005949:Glucose Oxidase