title: [Establishment and assessment of Caco-2 cell in vitro absorption model].
abstract: OBJECTIVE
  To establish and assess the Caco-2 cell in vitro absorption model.
  
  
  METHODS
  Caco-2 cells were cultured on the millipore filters fixed in Snapwell transport chamber. The cell morphology, transepithelial electrical resistance, mannitol efflux rate and alkaline phosphatase activities were monitored during culture.
  
  
  RESULTS
  After 21 days of in vitro culture, formation of tight junction was observed between the cells. The transepithelial electrical resistance reach a relatively stable value of 620-/+47 Omega.cm(2), the mannitol efflux rate was lower than 0.3%.h(-1).cm(-2), and the alkaline phosphatase activity in the apical side was significantly higher than that in the basolateral side.
  
  
  CONCLUSIONS
  The established Caco-2 cell model shows similar morphology to intestinal epithelial cells with formation of polarity, and can be used as an in vitro model for absorption studies.
authors.affiliation: Department of Nutrition and Food Hygiene, School of Public Health and Tropical Medicine, Southern Medical University, Guangzhou, China. lyzha@fimmu.com
authors.forename: Long-Ying
authors.initials: LY
authors.lastname: Zha
authors.forename: Hai-Ji
authors.initials: HJ
authors.lastname: Luo
authors.forename: Hong
authors.initials: H
authors.lastname: Deng
authors.forename: Xin-Wei
authors.initials: XW
authors.lastname: Chu
mesh_terms: D018938:Caco-2 Cells; D018929:Cell Culture Techniques / Q000379:methods*; D004847:Epithelial Cells / Q000166:cytology / Q000378:metabolism; D006801:Humans; D007408:Intestinal Absorption; D007422:Intestines / Q000166:cytology*