Prevalence of serum antibodies to TORCH among women before pregnancy or in the early period of pregnancy in Beijing.
BACKGROUND
The primary infection of Toxoplasma gondii (TOX), rubella virus (RV), cytomegalovirus (CMV), or herpes simplex virus (HSV), abbreviated as TORCH, remains a major problem in the pregnant women in China today.


METHODS
We analyzed the performance of a novel chemiluminesent immunoassay (CLIA) for anti-TORCH IgM and IgG detection then analyzed the prevalence of TORCH infection among 4692 women at childbearing age in Beijing, China.


RESULTS
The functional sensitivity of detecting anti-TOX IgM, anti-RV IgM, anti-CMV IgM and anti-HSV (types 1 and 2) IgM were 1.4, 3.2, 1.0 AU/ml and 0.5 index, respectively. The within-assay and the total CVs were both <10%, and recoveries of these assays ranged from 90-110%. High concentration of hemoglobin, lipids and bilirubin in sample did not affect the results. The infective rate of TORCH was 17.2%, with the highest positive rate of anti-HSV IgM. Within anti-TORCH IgG, anti-CMV IgG had the highest infective rate, 92.5%.


CONCLUSIONS
The infective rate of TORCH was fairly high among women before pregnancy or in the early period of pregnancy in Beijing. Entirely new approaches to prevention and treatment of congenital TORCH infection are necessary.
Li
Zhiyan
Z
Department of Clinical Laboratory, Peking University First Hospital, Beijing 100034, China.
Yan
Cuiling
C
Liu
Ping
P
Yan
Rong
R
Feng
Zhenru
Z
D000328:Adult; D044466:Asian People; D002681:China; D005260:Female; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D007132:Immunoglobulin Isotypes / Q000097:blood* / Q000276:immunology; D016014:Linear Models; D008163:Luminescent Measurements; D008875:Middle Aged; D011247:Pregnancy; D011248:Pregnancy Complications / Q000097:blood / Q000276:immunology / Q000382:microbiology* / Q000821:virology*; D011264:Pregnancy Trimesters; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013997:Time Factors; D014123:Toxoplasmosis / Q000276:immunology*; D014777:Virus Diseases / Q000276:immunology*
D007132:Immunoglobulin Isotypes