[Sensitivity of Jatropha curcas seeds to (60)Co-gamma radiation and their medial lethal doses in radiation breeding].
OBJECTIVE
To study the sensitivity of Jatropha curcas seeds from three different locations to (60)Co-gamma radiation and to determine the medial lethal doses (LD50) of (60)Co-gamma radiation for these seeds.


METHODS
Six different radiation doses (0, 100, 150, 200, 250 and 300 Gy) were used. Based on the germination rate 50%, LD50 doses of (60)Co-gamma radiation for the seeds were calculated using linear regression equation.


RESULTS
LD50 doses of (60)Co-gamma radiation for these seeds were 178 Gy (seeds from Guangdong), 132 Gy (seeds from Hainan) and 198 Gy (seeds from India) respectively. Increasing radiation doses caused more significant changes in leaf shape of the M1 seedlings.


CONCLUSIONS
The results provides an important experimental basis for the radiation breeding of the important herbal and energy plant J. curcas.
Wang
Zhao-Yu
ZY
Key Laboratory of Gene Engineering of the Ministry of Education, State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-Sen University, Guangzhou, China. wangzhaoyu77@yahoo.com.cn
Lin
Jing-Ming
JM
Luo
Li
L
Xu
Zeng-Fu
ZF
D003037:Cobalt Radioisotopes / Q000633:toxicity*; D005720:Gamma Rays; D018525:Germination / Q000528:radiation effects; D031286:Jatropha / Q000528:radiation effects*; D007928:Lethal Dose 50; D012639:Seeds / Q000528:radiation effects*
D003037:Cobalt Radioisotopes