title: Allelic variability in the third intron of the fibroin light chain gene in Bombyx mori (Lepidoptera: Bombycidae).
abstract: Conformation-sensitive gel electrophoresis is a useful method for identifying allele polymorphism; it provides co-dominant molecular markers. Using this method, we identified genetic variability in the third intron of the fibroin light chain gene, fib-L, in six Bombyx mori strains. Only Chinese C21A strain did not demonstrate allelic alterations, showing only homoduplex DNA molecules. We found distinct heteroduplex profiles in the Japanese HAA, M12B and M19-2 and the Chinese C25B and C24-2 strains. Analysis with restriction endonuclease fingerprinting conformation-sensitive gel electrophoresis demonstrated the potential of this method for the identification of allelic variability in B. mori; this was confirmed by cloning and sequencing the different alleles. The main alteration was a 12-bp deletion in two alleles of the C24-2 strain and one allele of the HAA strain; this deletion results in specific heteroduplex DNA molecule profiles.
authors.affiliation: Departamento de Biologia Celular e Genética, Universidade Estadual de Maringá, Maringá, PR, Brasil.
authors.forename: J F
authors.initials: JF
authors.lastname: Barbosa
authors.forename: J P
authors.initials: JP
authors.lastname: Bravo
authors.forename: D B
authors.initials: DB
authors.lastname: Zanatta
authors.forename: J L C
authors.initials: JL
authors.lastname: Silva
authors.forename: M A
authors.initials: MA
authors.lastname: Fernandez
mesh_terms: D000483:Alleles; D000818:Animals; D012831:Bombyx / Q000235:genetics*; D004247:DNA / Q000235:genetics; D005349:Fibroins / Q000235:genetics*; D007438:Introns / Q000235:genetics*; D011110:Polymorphism, Genetic
chemical_list: D004247:DNA; D005349:Fibroins