title: The in vitro nuclear aggregates of polyamines.
abstract: Natural polyamines (putrescine, spermidine, and spermine) self-assemble in a simulated physiological environment (50 mm sodium phosphate buffer, pH 7.2), generating in vitro nuclear aggregates of polyamines (ivNAPs). These supramolecular compounds are similar in structure and molecular mass to naturally occurring cellular nuclear aggregates of polyamines, and they share the ability of NAPs to interact with and protect the genomic DNA against nuclease degradation. Three main ivNAP compounds were separated by gel permeation chromatography. Their elution was carried out with 50 mm sodium phosphate buffer supplemented with 150 mm NaCl. Freezing and thawing of selected chromatographic fractions obtained by GPC runs in which the mobile phase was sodium phosphate buffer not supplemented with NaCl yielded three different microcrystallites, specifically corresponding to the ivNAPs, all of which were able to bind DNA. In this study, we demonstrated that in vitro self-assembly of polyamines and phosphates is a spontaneous, reproducible and inexpensive event, and provided the indications for the production of the ivNAPs as a new tool for manipulating the genomic DNA machinery.
authors.affiliation: Institute of Food Sciences, National Research Council (CNR), Avellino, Italy.
authors.forename: Aldo
authors.initials: A
authors.lastname: Di Luccia
authors.forename: Gianluca
authors.initials: G
authors.lastname: Picariello
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Iacomino
authors.forename: Annarita
authors.initials: A
authors.lastname: Formisano
authors.forename: Luigi
authors.initials: L
authors.lastname: Paduano
authors.forename: Luciano
authors.initials: L
authors.lastname: D'Agostino
mesh_terms: D002021:Buffers; D002850:Chromatography, Gel; D004247:DNA / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D011073:Polyamines / Q000737:chemistry* / Q000378:metabolism; D011700:Putrescine / Q000737:chemistry / Q000378:metabolism; D013095:Spermidine / Q000737:chemistry / Q000378:metabolism; D013096:Spermine / Q000737:chemistry / Q000378:metabolism
chemical_list: D002021:Buffers; D011073:Polyamines; D013096:Spermine; D004247:DNA; D013095:Spermidine; D011700:Putrescine