title: Developmental validation of the PrepFiler Forensic DNA Extraction Kit for extraction of genomic DNA from biological samples.
abstract: The PrepFiler Forensic DNA Extraction Kit enables isolation of genomic DNA from a variety of biological samples. The kit facilitates reversible binding of DNA with magnetic particles resulting in high DNA recovery from samples with very low and high quantities of biological materials: 0.1 and 40 microL of human blood (donor 2) provided 14 and 2883 ng of DNA, respectively. Following the revised SWGDAM guidelines, performance of the developed method was investigated using different sample types including saliva on swabs, semen stains on cotton fabric, samples exposed to environment, samples with polymerase chain reaction (PCR) inhibitors, blood stains (on denim, cotton cloth, and FTA paper), and touch evidence-type samples. DNA yields for all samples tested were equal or better than those obtained by both phenol-chloroform extraction and commercial kits tested. DNA obtained from these samples was free of detectable PCR inhibitors. Short tandem repeat profiles were complete, conclusive, and devoid of PCR artifacts.
authors.affiliation: Applied Biosystems Inc., 850 Lincoln Centre Drive, Foster City, CA 94404, USA.
authors.forename: Maxim G
authors.initials: MG
authors.lastname: Brevnov
authors.forename: Hemant S
authors.initials: HS
authors.lastname: Pawar
authors.forename: Janna
authors.initials: J
authors.lastname: Mundt
authors.forename: Lisa M
authors.initials: LM
authors.lastname: Calandro
authors.forename: Manohar R
authors.initials: MR
authors.lastname: Furtado
authors.forename: Jaiprakash G
authors.initials: JG
authors.lastname: Shewale
mesh_terms: D001769:Blood; D004247:DNA / Q000032:analysis*; D016172:DNA Fingerprinting / Q000295:instrumentation* / Q000379:methods; D005260:Female; D006801:Humans; D008280:Magnetics; D008297:Male; D016133:Polymerase Chain Reaction; D015203:Reproducibility of Results; D012463:Saliva; D012661:Semen; D020080:Tandem Repeat Sequences
chemical_list: D004247:DNA