A new methodology for testing chemicals and drugs on cell activity.
Synopsis A new method for testing the effects of cosmetics and various chemicals on cell activity is described. It is based on the study of mitochondria compartments and combines the use of fluorescent probes which are accumulated in a potential-dependent (Rhodamine 123) or potential-independent (Nonyl Acridine Orange) manner with flow cytometric analysis. Measured fluorescences allow the determination of a new parameter indicating mitochondria efficiency. This new methodology for the evaluation of cell activity has been applied in three different situations: during cell ageing, during cell cycle and when cells are in the presence of drugs.
Maftah
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Genius-Biotechnologie, UFR des Sciences, 123, Avenue Albert Thomas, 87060 Limoges Cedex, France.
Petit
J M
JM
Leprat
P
P
Ratinaud
M H
MH
Julien
R
R