MEK1-independent activation of MAPK and MEK1-dependent activation of p70 S6 kinase by stem cell factor (SCF) in ovarian cancer cells.
We discovered a stem cell factor (SCF)-triggered, MEK1-independent, and PI3K-dependent MAPK activation pathway in the Kit-expressing ovarian cancer cell line HEY. When we knocked down MEK1 with RNA interference (RNAi) to study the function of MEK1 on the proliferation and survival of ovarian cancer cells, we found that impaired cell growth still occurred after MEK1 expression had been suppressed, although MAPK activation remained intact. This suggests that there is MEK1-independent activation of MAPK in the SCF-induced ovarian cancer cell growth process, and that MEK1 still plays a crucial role in maintaining the malignant properties of ovarian cancer cells even when it fails to activate MAPK as expected.
Liu
Lian
L
Department of Chemotherapy, Cancer Center, Qilu Hospital, Shandong University, Jinan, China. tounao@126.com
Zhang
Xin
X
Du
Chao
C
Zhang
Xiaoning
X
Hou
Nan
N
Zhao
Di
D
Sun
Jianzhi
J
Li
Li
L
Wang
Xiuwen
X
Ma
Chunhong
C
D045744:Cell Line, Tumor; D049109:Cell Proliferation; D004789:Enzyme Activation; D005260:Female; D055785:Gene Knockdown Techniques; D006801:Humans; D048369:MAP Kinase Kinase 1 / Q000235:genetics / Q000378:metabolism*; D020929:Mitogen-Activated Protein Kinase Kinases / Q000378:metabolism*; D010051:Ovarian Neoplasms / Q000201:enzymology / Q000473:pathology*; D034622:RNA Interference; D038762:Ribosomal Protein S6 Kinases, 70-kDa / Q000378:metabolism*; D019089:Stem Cell Factor / Q000378:metabolism* / Q000494:pharmacology
D019089:Stem Cell Factor; D038762:Ribosomal Protein S6 Kinases, 70-kDa; D048369:MAP Kinase Kinase 1; C482125:MAP2K1 protein, human; D020929:Mitogen-Activated Protein Kinase Kinases