title: Incorporation of an inducible nucleotide analog into DNA by DNA polymerases.
abstract: Non-natural nucleotides with diverse functionalities are highly useful in many areas of basic research and practical applications. We have previously developed an efficient method for post-synthetic modifications of 2-amino-6-vinylpurine (AVP)-containing oligonucleotides, which permits conjugations of a variety of useful functional appendages to the AVP moiety in DNA. Here we report an investigation on the ability of various DNA polymerases to use 5'-triphosphate of 2'-deoxyribosyl-2-amino-6-(2-methylthioethyl)purine (a stable precursor of AVP) as the substrate for templated DNA synthesis.
authors.affiliation: Department of Biochemistry and Biomedical Sciences, McMaster University, 1200 Main Street West, Hamilton, Canada ON L8N 3Z5.
authors.forename: Md Monsur
authors.initials: MM
authors.lastname: Ali
authors.forename: Shuhei
authors.initials: S
authors.lastname: Imoto
authors.forename: Yingfu
authors.initials: Y
authors.lastname: Li
authors.forename: Shigeki
authors.initials: S
authors.lastname: Sasaki
authors.forename: Fumi
authors.initials: F
authors.lastname: Nagatsugi
mesh_terms: D001483:Base Sequence; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism*; D009838:Oligodeoxyribonucleotides / Q000096:biosynthesis* / Q000737:chemistry; D011685:Purine Nucleotides / Q000138:chemical synthesis* / Q000737:chemistry; D013698:Templates, Genetic
chemical_list: C528377:2'-deoxyribosyl-2-amino-6-(2-methylthioethyl)purine nucleoside triphosphate; D009838:Oligodeoxyribonucleotides; D011685:Purine Nucleotides; D004259:DNA-Directed DNA Polymerase