Integrated Raman spectroscopy and trimodal wide-field imaging techniques for real-time in vivo tissue Raman measurements at endoscopy.
We report an integrated Raman spectroscopy and trimodal (white-light reflectance, autofluorescence, and narrow-band) imaging techniques for real-time in vivo tissue Raman measurements at endoscopy. A special 1.8 mm endoscopic Raman probe with filtering modules is developed, permitting effective elimination of interference of fluorescence background and silica Raman in fibers while maximizing tissue Raman collections. We demonstrate that high-quality in vivo Raman spectra of upper gastrointestinal tract can be acquired within 1 s or subseconds under the guidance of wide-field endoscopic imaging modalities, greatly facilitating the adoption of Raman spectroscopy into clinical research and practice during routine endoscopic inspections.
Huang
Zhiwei
Z
Bioimaging Laboratory, Department of Bioengineering, Faculty of Engineering, National University of Singapore, and National University Hospital, Singapore. biehzw@nus.edu.sg
Teh
Seng Khoon
SK
Zheng
Wei
W
Mo
Jianhua
J
Lin
Kan
K
Shao
Xiaozhuo
X
Ho
Khek Yu
KY
Teh
Ming
M
Yeoh
Khay Guan
KG
D003199:Computer Systems; D019723:Endoscopes; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005336:Fiber Optic Technology / Q000295:instrumentation*; D006801:Humans; D007089:Image Enhancement / Q000379:methods*; D009061:Mouth Mucosa / Q000166:cytology*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013059:Spectrum Analysis, Raman / Q000295:instrumentation*; D018511:Systems Integration