Effects of in vitro lactoferricin and lactoferrin on the head kidney cells of European sea bass (Dicentrarchus labrax, L.).
Antimicrobial, anti-inflammatory and immunomodulating properties of lactoferrin have been demonstrated in mammals and in fish. However, in vivo, lactoferrin is digested by gastric pepsin treatment into the N-terminal derived peptide named lactoferricin. This has been so far overlooked in fish in vitro studies. The aim of the present study was to assess in vitro the effects of both lactoferricin and lactoferrin on the head kidney cells of European sea bass (Dicentrarchus labrax, L.) in order to determine their potential as dietary additives and to get some insight into their mode of action. In vitro lactoferricin decreased significantly the chemiluminescent response of head kidney cells but did not affect the zymosan-triggered chemiluminescence activity. On the other hand, a high concentration of lactoferrin directly stimulated chemiluminescence but reduced the zymosan-triggered chemiluminescence. The bactericidal activity of head kidney cells was also significantly diminished by pre-incubation with lactoferrin in a dose-dependent manner. Although no significant effect of lactoferricin or lactoferrin was evidenced on head kidney cellular viability, absent or negative effect on the priming of respiratory burst activity suggested that care should be taken when using lactoferrin in the diet of sea bass and high doses should be avoided. Hypotheses about the mechanisms of action of lactoferricin and lactoferrin are presented.
Henry
Morgane A
MA
Department of Fish Nutrition and Pathology, Institute of Aquaculture, Hellenic Centre for Marine Research, Aghios Kosmas, Elliniko 16604, Greece. morgane@ath.hcmr.gr
Alexis
Maria N
MN
D000821:Animal Feed / Q000009:adverse effects; D000818:Animals; D001492:Bass / Q000276:immunology / Q000378:metabolism*; D002470:Cell Survival / Q000187:drug effects; D004926:Escherichia coli / Q000276:immunology / Q000472:pathogenicity; D005503:Food Additives / Q000494:pharmacology; D066298:In Vitro Techniques; D007668:Kidney / Q000166:cytology / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism*; D007781:Lactoferrin / Q000008:administration & dosage / Q000009:adverse effects / Q000494:pharmacology*; D049449:Luminescence; D016897:Respiratory Burst / Q000187:drug effects
D005503:Food Additives; C082171:lactoferricin B; D007781:Lactoferrin