title: Skin atrophy in cytoplasmic SOD-deficient mice and its complete recovery using a vitamin C derivative.
abstract: Intrinsic skin ageing is characterized by atrophy and loss of elasticity. Although the skin hypertrophy induced by photoageing has been studied, the molecular mechanisms of skin atrophy during ageing remain unclear. Here, we report that copper/zinc superoxide dismutase (CuZn-SOD)-deficient mice show atrophic morphology in their skin. This atrophy is accompanied by the degeneration of collagen and elastic fibers, and skin hydroxyproline is also significantly reduced in deficient mice. These imply that the dysfunction of collagen and elastin biosynthesis are involved in the progression of skin thinning. Furthermore, transdermal administration of a vitamin C derivative which can permeate through the membrane, completely reversed the skin thinning and deterioration of collagen and elastin in the mutant mice. These indicate that the vitamin C derivative is a powerful agent for alleviating skin ageing through regeneration of collagen and elastin. The CuZn-SOD-deficient mice might be applicable to evaluation of therapeutic medicines against skin ageing.
authors.affiliation: Research Team for Molecular Biomarkers, Tokyo Metropolitan Institute of Gerontology, Tokyo, Japan.
authors.forename: Kazuma
authors.initials: K
authors.lastname: Murakami
authors.forename: Jun
authors.initials: J
authors.lastname: Inagaki
authors.forename: Mitsuru
authors.initials: M
authors.lastname: Saito
authors.forename: Yasutaka
authors.initials: Y
authors.lastname: Ikeda
authors.forename: Chizuru
authors.initials: C
authors.lastname: Tsuda
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Noda
authors.forename: Satoru
authors.initials: S
authors.lastname: Kawakami
authors.forename: Takuji
authors.initials: T
authors.lastname: Shirasawa
authors.forename: Takahiko
authors.initials: T
authors.lastname: Shimizu
mesh_terms: D000818:Animals; D001205:Ascorbic Acid / Q000031:analogs & derivatives* / Q000494:pharmacology / Q000627:therapeutic use; D001284:Atrophy / Q000188:drug therapy / Q000235:genetics / Q000473:pathology; D003094:Collagen / Q000378:metabolism; D003593:Cytoplasm; D004549:Elastin / Q000378:metabolism; D051379:Mice; D018345:Mice, Knockout; D012867:Skin / Q000187:drug effects* / Q000201:enzymology / Q000473:pathology; D015595:Skin Aging / Q000187:drug effects* / Q000235:genetics; D013482:Superoxide Dismutase / Q000235:genetics*; D000072105:Superoxide Dismutase-1
chemical_list: C403966:6-O-palmitol-ascorbate-2-O-phosphate; D003094:Collagen; D004549:Elastin; C000606291:Sod1 protein, mouse; D013482:Superoxide Dismutase; D000072105:Superoxide Dismutase-1; D001205:Ascorbic Acid