Quantification of water buffalo (Bubalus bubalis) cytokine expression in response to inactivated foot-and-mouth disease (FMD) vaccine.
This study describes the quantification of cytokine expression of vaccinated water buffaloes with FMD inactivated vaccine. Using real-time PCR quantification assay, expression of Th1 (IL-2, IL-12p40, IFNgamma); Th2 (IL-4, IL-10) and inflammatory (IL-6, TNFalpha) cytokines were quantified weekly for the entire three-week duration of the experiment. It was noted that IFNgamma, IL-10 and TNFalpha had peaked on week three post-vaccination while the remaining cytokines peaked on the second week and decreased by the third week. The counteraction between IFNgamma and IL-4 was noted as well as the possible suppressive action of IL-10 to that of IL-2 and IL-12, which is a common phenomenon between Th1 and Th2 cytokines. Synergy between TNFa and IL-6 was also observed. These findings suggest that within the immune system of water buffalo there is a dynamic cell-mediated and humoral interaction in response to immunogen. This assessment of the cytokine expressions is vital for the study of water buffalo disease progression and concurring protective immune responses.
Mingala
Claro N
CN
Laboratory of Infectious Diseases, Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University, Hokkaido 060-0818, Japan.
Konnai
Satoru
S
Venturina
Fe A
FA
Onuma
Misao
M
Ohashi
Kazuhiko
K
D000818:Animals; D000917:Antibody Formation; D002020:Buffaloes / Q000276:immunology*; D016207:Cytokines / Q000235:genetics*; D004357:Drug Synergism; D005536:Foot-and-Mouth Disease / Q000235:genetics / Q000276:immunology*; D007111:Immunity, Cellular; D007165:Immunosuppression Therapy; D016753:Interleukin-10 / Q000276:immunology; D018664:Interleukin-12 / Q000276:immunology; D007376:Interleukin-2 / Q000276:immunology; D014409:Tumor Necrosis Factor-alpha / Q000276:immunology / Q000627:therapeutic use; D015164:Vaccines, Inactivated / Q000627:therapeutic use*
D016207:Cytokines; D007376:Interleukin-2; D014409:Tumor Necrosis Factor-alpha; D015164:Vaccines, Inactivated; D016753:Interleukin-10; D018664:Interleukin-12