Does mobilization for autologous stem cell transplantation damage stromal layer formation?
Autologous hematopoietic stem cell transplantation (HSCT) has proved efficient to treat hematological malignancies. However, some patients fail to mobilize HSCs. It is known that the microenvironment may undergo damage after allogeneic HSCT. However little is known about how chemotherapy and growth factors contribute to this damage. We studied the stromal layer formation (SLF) and velocity before and after HSC mobilization, through long-term bone marrow culture from 22 patients and 10 healthy donors. Patients' SLF was similar at pre- (12/22) and post-mobilization (9/20), however for controls this occurred more at pre-mobilization (9/10; p=0.03). SLF velocity was higher at pre than post-mobilization in both groups. Leukemias and multiple myeloma showed faster growth of SLF than lymphomas at post-mobilization, the latter being similar to controls. These findings could be explained by less uncommitted HSC in controls than patients at post-mobilization. Control HSCs may migrate more in response to mobilization, resulting in a reduced population by those cells.
Schimieguel
Dulce Marta
DM
Discipline of Hematology and Hemotherapy, Medicine Department, Federal University of São Paulo, Brazil. dulcemarta@hotmail.com
Dominato
Juliana Augusta Albieri
JA
Zattar
Karin Cecyn
KC
Silva
Maria Regina Régis
MR
de Souza
Marlon Knabben
MK
Nader
Helena Bonciani
HB
Borelli
Primavera
P
de Oliveira
José Salvador Rodrigues
JS
D000293:Adolescent; D000328:Adult; D018952:Antigens, CD34 / Q000378:metabolism; D001854:Bone Marrow Cells / Q000378:metabolism; D002470:Cell Survival; D002478:Cells, Cultured; D005260:Female; D016179:Granulocyte Colony-Stimulating Factor / Q000008:administration & dosage; D019650:Hematopoietic Stem Cell Mobilization / Q000379:methods*; D018380:Hematopoietic Stem Cell Transplantation / Q000379:methods*; D006801:Humans; D008297:Male; D008858:Microscopy, Phase-Contrast; D008875:Middle Aged; D017154:Stromal Cells / Q000166:cytology* / Q000378:metabolism; D014182:Transplantation, Autologous; D055815:Young Adult
D018952:Antigens, CD34; D016179:Granulocyte Colony-Stimulating Factor