title: Automation of the BD GeneOhm methicillin-resistant Staphylococcus aureus assay for high-throughput screening of nasal swab specimens.
abstract: This study demonstrated that an automated version of the BD-GeneOhm methicillin-resistant Staphylococcus aureus (MRSA) assay (BD-MRSA), using achromopeptidase sample lysis and PCR setup performed on the Hamilton MICROLAB STAR(let) (Auto-MRSA), gave results comparable to those obtained with BD-MRSA. The positive- and negative-result concordance rates and overall concordance of BD-MRSA and Auto-MRSA were 98.2, 97.7, and 97.6%, respectively. Auto-MRSA required 60% less technical time than BD-MRSA, and the time required to obtain results was 50% of that required for BD-MRSA. Auto-MRSA provides a reliable, robust system for high-volume clinical use.
authors.affiliation: Department of Laboratory Medicine, Molecular Diagnostics, North Shore, LIJ Health System Laboratories, Lake Success, New York 11042, USA.
authors.forename: Xue-Ping
authors.initials: XP
authors.lastname: Wang
authors.forename: Christine C
authors.initials: CC
authors.lastname: Ginocchio
mesh_terms: D001331:Automation; D002353:Carrier State / Q000175:diagnosis* / Q000382:microbiology; D006801:Humans; D008403:Mass Screening / Q000379:methods*; D055624:Methicillin-Resistant Staphylococcus aureus / Q000302:isolation & purification*; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D012680:Sensitivity and Specificity; D013203:Staphylococcal Infections / Q000175:diagnosis* / Q000382:microbiology