Simultaneous HPLC-UV determination of rhein and aceclofenac in human plasma.
Diacerein and aceclofenac are prescribed for reducing the symptoms associated with osteoarthritis. We present a simple HPLC method with UV detection for simultaneous determination of rhein (the immediate metabolite of diacerein) and aceclofenac from human plasma samples. Sample preparation was accomplished through liquid-liquid extraction with ethyl acetate and chromatographic separation was performed on a reversed-phase ODS column. Mobile phase consisted of a mixture of acetate buffer and acetonitrile run under gradient at flow rate of 1.0 ml/min. Wavelength was set at 258 nm. The method was validated for linearity, accuracy, precision and stability. The calibration was linear over the range of 0.1-7.0 microg/ml for rhein and 0.5-20 microg/ml for aceclofenac using 500 microl plasma samples. Extraction recoveries were 85% for rhein and 70% for aceclofenac. The method can easily be adopted for high-throughput clinical and pharmacokinetic studies of above two-drug fixed dose combination formulations.
Ojha
Ashwini
A
B.V. Patel Pharmaceutical Education Research and Development Centre (PERD), Thaltej, Ahmedabad, Gujarat, India.
Rathod
Rajeshwari
R
Padh
Harish
H
D000880:Anthraquinones / Q000097:blood*; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000097:blood*; D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D004008:Diclofenac / Q000031:analogs & derivatives* / Q000097:blood; D004791:Enzyme Inhibitors / Q000097:blood*; D006801:Humans; D007202:Indicators and Reagents; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet
D000880:Anthraquinones; D000894:Anti-Inflammatory Agents, Non-Steroidal; D004791:Enzyme Inhibitors; D007202:Indicators and Reagents; D004008:Diclofenac; C056498:aceclofenac; C020491:rhein