Gonadal sex differentiation and expression of Sox9a2, Dmrt1, and Foxl2 in Oryzias luzonensis.
Oryzias luzonensis is closely related to the medaka, O. latipes. The sex of both species is determined by an XX-XY system. However, the testis determining gene (DMY/Dmrt1bY) found in O. latipes does not exist in O. luzonensis. Instead, a different gene is thought to act as a testis determining gene. In this study, we focused the gonadal sex differentiation process in O. luzonensis under different testis determining gene. First, we observed the gonadal development of O. luzonensis histologically. We then analyzed the expression of Sox9a2/Sox9b, Dmrt1, and Foxl2 during early development. Our results suggest that the sexual differentiation of germ cells in O. luzonensis is initiated later than in O. latipes. However, the timing of the sexual differentiation of the supporting cell linage is similar between the species.
Nakamoto
Masatoshi
M
Department of Biology, Shinshu University, Matsumoto, Nagano, Japan.
Muramatsu
Shinsuke
S
Yoshida
Shoko
S
Matsuda
Masaru
M
Nagahama
Yoshitaka
Y
Shibata
Naoki
N
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D005260:Female; D029941:Fish Proteins / Q000145:classification / Q000235:genetics*; D051858:Forkhead Transcription Factors / Q000145:classification / Q000235:genetics; D018507:Gene Expression Regulation, Developmental; D006066:Gonads / Q000033:anatomy & histology / Q000254:growth & development / Q000378:metabolism*; D017403:In Situ Hybridization; D008297:Male; D008969:Molecular Sequence Data; D009990:Oryzias / Q000235:genetics* / Q000254:growth & development; D010053:Ovary / Q000033:anatomy & histology / Q000254:growth & development / Q000378:metabolism; D010802:Phylogeny; D020133:Reverse Transcriptase Polymerase Chain Reaction; D055758:SOX9 Transcription Factor / Q000145:classification / Q000235:genetics; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D012733:Sex Differentiation / Q000235:genetics*; D013737:Testis / Q000033:anatomy & histology / Q000254:growth & development / Q000378:metabolism; D014157:Transcription Factors / Q000145:classification / Q000235:genetics
C110547:DMRT1 protein; D018076:DNA, Complementary; D029941:Fish Proteins; D051858:Forkhead Transcription Factors; D055758:SOX9 Transcription Factor; D014157:Transcription Factors