Differential expression profile of microRNAs in human placentas from preeclamptic pregnancies vs normal pregnancies.
OBJECTIVE
The purpose of this study was to perform a comprehensive analysis of the microRNA expression profile in placentas from preeclamptic pregnancies vs normal placentas.


METHODS
Placentas were obtained from patients with (1) mild preeclampsia (n = 8) and (2) severe preeclampsia (n = 15) and (3) in a normal control group (n = 11) with elective cesarean delivery. The microRNA expression profile was assessed by microRNA microarray and real-time reverse transcriptase-polymerase chain reaction analysis.


RESULTS
Thirty-four microRNAs were expressed differentially in preeclamptic placentas, compared with normal placentas. Of these, 11 microRNAs were overexpressed, and 23 microRNAs were underexpressed in preeclamptic pregnancies. Notably, several microRNA clusters on human chromosome 19q13.42, 13q31.3, Xq26.2, Xq26.3, and 14q32.31 (a human imprinted region) were expressed differentially in preeclamptic placentas. These results were confirmed with the use of real-time polymerase chain reaction for selected microRNAs (miR-210, -152, -411, and so on).


CONCLUSIONS
The results show that 34 microRNAs are deregulated in preeclamptic pregnancies, which suggests the involvement of these microRNAs in the pathogenesis of preeclampsia.
Zhu
Xiao-ming
XM
Department of Obstetrics and Gynecology, Tangdu Hospital, The Fourth Military Medical University, Xi'an, China.
Han
Tao
T
Sargent
Ian L
IL
Yin
Guo-wu
GW
Yao
Yuan-qing
YQ
D000328:Adult; D005260:Female; D015870:Gene Expression; D006801:Humans; D035683:MicroRNAs / Q000235:genetics*; D010920:Placenta; D011225:Pre-Eclampsia / Q000235:genetics*; D011247:Pregnancy; D012720:Severity of Illness Index
D035683:MicroRNAs