title: In situ molecular level studies on membrane related peptides and proteins in real time using sum frequency generation vibrational spectroscopy.
abstract: Sum frequency generation (SFG) vibrational spectroscopy has been demonstrated to be a powerful technique to study the molecular structures of surfaces and interfaces in different chemical environments. This review summarizes recent SFG studies on hybrid bilayer membranes and substrate-supported lipid monolayers and bilayers, the interaction between peptides/proteins and lipid monolayers/bilayers, and bilayer perturbation induced by peptides/proteins. To demonstrate the ability of SFG to determine the orientations of various secondary structures, studies on the interactions between different peptides/proteins (melittin, G proteins, alamethicin, and tachyplesin I) and lipid bilayers are discussed. Molecular level details revealed by SFG in these studies show that SFG can provide a unique understanding on the interactions between a lipid monolayer/bilayer and peptides/proteins in real time, in situ and without any exogenous labeling.
authors.affiliation: Department of Chemistry, University of Michigan, Ann Arbor, 48109, USA.
authors.forename: Shuji
authors.initials: S
authors.lastname: Ye
authors.forename: Khoi Tan
authors.initials: KT
authors.lastname: Nguyen
authors.forename: Stéphanie V
authors.initials: SV
authors.lastname: Le Clair
authors.forename: Zhan
authors.initials: Z
authors.lastname: Chen
mesh_terms: D000818:Animals; D006801:Humans; D008051:Lipid Bilayers / Q000737:chemistry; D008565:Membrane Proteins / Q000737:chemistry; D010455:Peptides / Q000737:chemistry; D011506:Proteins / Q000737:chemistry*; D013057:Spectrum Analysis / Q000379:methods*; D014732:Vibration
chemical_list: D008051:Lipid Bilayers; D008565:Membrane Proteins; D010455:Peptides; D011506:Proteins