title: Genotyping of IRGM tetranucleotide promoter oligorepeats by fluorescence resonance energy transfer.
abstract: Fluorescence resonance energy transfer (FRET) genotyping has been well established for the rapid assessment of single nucleotide polymorphisms (SNPs) and deletions. A design is presented that allows the typing of short tandem oligo repeat sequences using the LightTyper/LightCycler system. The protocol was evaluated and applied to the typing of a tetranucleotide promoter repeat of the human gene encoding the immunity-related GTPase family, M (IRGM) molecule in >2000 individuals from Ghana, West Africa.
authors.affiliation: Bernhard Nocht Institute for Tropical Medicine, Department of Molecular Medicine, Hamburg, Germany.
authors.forename: Christopher D
authors.initials: CD
authors.lastname: Intemann
authors.forename: Thorsten
authors.initials: T
authors.lastname: Thye
authors.forename: Jürgen
authors.initials: J
authors.lastname: Sievertsen
authors.forename: Ellis
authors.initials: E
authors.lastname: Owusu-Dabo
authors.forename: Rolf D
authors.initials: RD
authors.lastname: Horstmann
authors.forename: Christian G
authors.initials: CG
authors.lastname: Meyer
mesh_terms: D001483:Base Sequence; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods*; D019204:GTP-Binding Proteins / Q000235:genetics*; D005838:Genotype; D005869:Ghana; D006801:Humans; D018895:Microsatellite Repeats; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic
chemical_list: D019204:GTP-Binding Proteins; C513539:IRGM protein, human