Histopathological changes and tumour necrosis factor-alpha, transforming growth factor-beta and tenascin expression in patients with primary type I membranoproliferative glomerulonephritis in remission.
OBJECTIVE
Primary type I membranoproliferative glomerulonephritis (MPGN) is a rare cause of glomerular disease with a high relapse rate and poor prognosis. The aim of this study was: (i) to evaluate the histopathological findings associated with remission; and (ii) to document the possible clinical and histopathological factors predicting relapses.


METHODS
Eleven type I MPGN patients (five men, six women; mean age, 38.8+/-13.5 years) who were in remission for at least 1 year after the cessation of immunosuppressive drugs were re-biopsied. The intensity of immunostaining for tumor necrosis factor (TNF)-alpha, transforming growth factor (TGF)-beta1, and tenascin was graded from 0 (no staining) to 3+ (maximum staining).


RESULTS
Mean baseline mesangial cellularity score and tubulointerstitial infiltration score were reduced and mesangial matrix expansion score was increased at protocol re-biopsies compared to baseline. The glomerular and tubulointerstitial staining scores for TGF-beta1 and tenascin were higher than that of baseline. Reduced tubulointerstitial TNF-alpha expression was found in re-biopsy specimens compared to baseline. Patients have been followed for a mean time of 51.5+/-22.2 months after the protocol biopsy. Eight patients had a relapse. Mesangial cellularity score and glomerular tenascin expression at re-biopsy specimens were higher in relapsed patients compared to those without a relapse.


CONCLUSIONS
Our study shows that mesangial cellularity and tubulointerstitial cell infiltration are reducing whereas mesangial matrix expansion, glomerular and tubulointerstitial TGF-beta1 and tenascin expression are increasing with remission. The higher mesangial cell proliferation and glomerular tenascin scores in remission are associated with the development of relapse.
Arikan
Hakki
H
Division of Nephrology, Department of Medicine, Marmara Medical School, Istanbul, Turkey. ihakkiarikan@yahoo.com
Koc
Mehmet
M
Cakalagaoglu
Fulya
F
Tuglular
Serhan
S
Ozener
Cetin
C
Akoglu
Emel
E
D000328:Adult; D001706:Biopsy; D005260:Female; D015432:Glomerulonephritis, Membranoproliferative / Q000378:metabolism / Q000473:pathology*; D006801:Humans; D007668:Kidney / Q000737:chemistry / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D019063:Tenascin / Q000032:analysis*; D053773:Transforming Growth Factor beta1 / Q000032:analysis*; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis*
D019063:Tenascin; D053773:Transforming Growth Factor beta1; D014409:Tumor Necrosis Factor-alpha