Expression and localization of regenerating gene I in a rat liver regeneration model.
Regenerating gene (Reg) I has been identified as a regenerative/proliferative factor for pancreatic islet cells. We examined Reg I expression in the regenerating liver of a rat model that had been administered 2-acetylaminofluorene and treated with 70% partial hepatectomy (2-AAF/PH model), where hepatocyte and cholangiocyte proliferation was suppressed and the hepatic stem cells and/or hepatic progenitor cells were activated. In a detailed time course study of activation of hepatic stem cells in the 2-AAF/PH model, utilizing immunofluorescence staining with antibodies of Reg I and other cell-type-specific markers, we found that Reg I-expressing cells are present in the bile ductules and increased during regeneration. Reg I-expressing cells were colocalized with CK19, OV6, and AFP. These results demonstrate that Reg I is significantly upregulated in the liver of the 2-AAF/PH rat model, accompanied by the formation of bile ductules during liver regeneration.
Wang
Jingshu
J
Department of Biochemistry, Akita University School of Medicine, 1-1-1 Hondo, Akita 010-8543, Japan.
Koyota
Souichi
S
Zhou
Xiaoping
X
Ueno
Yasuharu
Y
Ma
Li
L
Kawagoe
Masami
M
Koizumi
Yukio
Y
Okamoto
Hiroshi
H
Sugiyama
Toshihiro
T
D000818:Animals; D001652:Bile Ducts / Q000378:metabolism; D015870:Gene Expression; D051847:Lithostathine / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D008099:Liver / Q000378:metabolism*; D008115:Liver Regeneration; D008297:Male; D023421:Models, Animal; D051381:Rats; D011916:Rats, Inbred F344; D015854:Up-Regulation
D051847:Lithostathine; C497616:Reg1a protein, rat