Ptpcd-1 is a novel cell cycle related phosphatase that regulates centriole duplication and cytokinesis.
Proper progression of mitosis requires spatio-temporal regulation of protein phosphorylation by orchestrated activities of kinases and phosphatases. Although many kinases, such as Aurora kinases, polo-like kinases (Plks), and cyclin B-Cdk1 are relatively well characterized in the context of their physiological functions at mitosis and regulation of their enzymatic activities during mitotic progression, phosphatases involved are largely unknown. Here we identified a novel protein tyrosine phosphatase containing domain 1 (Ptpcd 1) as a mitotic phosphatase, which shares sequence homology to Cdc14. Immunofluorescence studies revealed that Ptpcd1 partially colocalized with gamma-tubulin, an archetypical centrosomal marker. Overexpression of this phosphatase prevented unscheduled centrosomal amplification in hydroxyurea arrested U2OS cells. Intriguingly, Ptpcd 1-associated and colocalized with polo-like kinase 1(Plk1). Hence, overexpression of Ptpcd1 rescued prometaphase arrest of Plk-1 depleted cells, but resulted in aberrant cytokinesis as did as Plk1 overexpression. These results suggested that Ptpcd1 is involved in centrosomal duplication and cytokinesis.
Zineldeen
Doaa H
DH
Department of Cell Biology and Biochemistry, Graduate School of Medical Sciences, Nagoya City University, 1 Kawasumi, Mizuho-cho, Mizuho-ku, Nagoya 467-8601, Japan.
Shimada
Midori
M
Niida
Hiroyuki
H
Katsuno
Yuko
Y
Nakanishi
Makoto
M
D000818:Animals; D002453:Cell Cycle / Q000235:genetics; D018797:Cell Cycle Proteins / Q000235:genetics / Q000378:metabolism*; D002502:Centrioles / Q000201:enzymology*; D003001:Cloning, Molecular; D048749:Cytokinesis / Q000235:genetics; D005816:Genetic Complementation Test; D006367:HeLa Cells; D006801:Humans; D051379:Mice; D008938:Mitosis / Q000235:genetics; D010744:Phosphoric Monoester Hydrolases / Q000235:genetics / Q000378:metabolism*; D017346:Protein Serine-Threonine Kinases / Q000235:genetics / Q000378:metabolism; D017027:Protein Tyrosine Phosphatases / Q000235:genetics / Q000378:metabolism*; D011518:Proto-Oncogene Proteins / Q000235:genetics / Q000378:metabolism; D000097869:Polo-Like Kinase 1
D018797:Cell Cycle Proteins; D010744:Phosphoric Monoester Hydrolases; D017346:Protein Serine-Threonine Kinases; D017027:Protein Tyrosine Phosphatases; D011518:Proto-Oncogene Proteins; D000097869:Polo-Like Kinase 1; C539912:Ptpcd-1 protein, mouse