In vivo lamellar keratoplasty using platelet-rich plasma as a bioadhesive.
OBJECTIVE
The use of standard sutures has been replaced by platelet-rich plasma (PRP), a bioadhesive agent, in several surgical procedures. This prompted us to test PRP efficacy in experimental lamellar keratoplasty.


METHODS
After lamellar anterior keratoplasty, PRP with a mean concentration of 807,564 platelets/mm(3) was used to attach the corneal flap to the stromal surface in 12 New Zealand white rabbits. 10-0 nylon sutures were used in one control group of 12 animals and no suture was used in a second control group of six rabbits. Animals were killed at days 2, 7, 30, and 90 for histological and smooth muscle actin (SMA) immunohistochemical analysis.


RESULTS
The PRP group showed a tight corneal graft from the first postoperative hours until they were killed. A transparent cornea was seen at 30 days and remained clear until the end of the protocol. Histological specimens showed no signs of ocular inflammation in any animal within the PRP group. Electron microscopy showed normal morphological features on the flap and stromal bed, and a clear interface zone without cells or debris. The number of stromal myofibroblasts was lower than that seen in the suture group at 3 months postsurgery. The sutured group showed an attached cornea with signs of inflammation around the knots. All flaps without PRP or sutures were completely detached in the immediate postoperative period.


CONCLUSIONS
PRP was useful for attaching the corneal flap and it was well tolerated by the rabbit corneal tissue. Corneal healing was satisfactory. Further studies on PRP adhesiveness in grafts with donor corneas should be performed before considering its use in patients.
Luengo Gimeno
F
F
Ophthalmology Department, Facultad de Ciencias Biomédicas, Universidad Austral, Pilar, Argentina.
Gatto
S C
SC
Croxatto
J O
JO
Ferro
J I
JI
Gallo
J E
JE
D000818:Animals; D001672:Biocompatible Materials / Q000627:therapeutic use*; D003315:Cornea / Q000276:immunology / Q000473:pathology; D016039:Corneal Transplantation / Q000379:methods*; D007150:Immunohistochemistry; D053657:Platelet-Rich Plasma; D011184:Postoperative Period; D011817:Rabbits; D017154:Stromal Cells / Q000473:pathology; D014014:Tissue Adhesives / Q000627:therapeutic use*
D001672:Biocompatible Materials; D014014:Tissue Adhesives