Dehalogenation of gaseous 1-chlorobutane by dehydrated whole cells: Influence of the microenvironment of the halidohydrolase on the stability of the biocatalyst.
It was observed that a biocatalyst prepared from dehydrated whole cells of a recombinant Escherichia coli (initially suspended in borate buffer) was able to hydrolyze gaseous 1-chlorobutane in a solid/gas reactor. Nevertheless, at 40 degrees C and for a 0.7 water activity, it rapidly lost its activity. The explanation of this phenomenon was first investigated by observing the biocatalyst structure at the microscopic level and by studying the localization of the dehalogenase involved in catalysis (intracellular/extracellular). The behavior of this biocatalyst was then compared with that of a preparation made from cells extracts. The reasons of the inactivation are discussed in terms of thermal denaturation and protective effect of buffer salts.
Marchand
P
P
LIENSs-LIttoral Environnement et Sociétés, Université de La Rochelle-UMR 6250-CNRS, Avenue Michel Crépeau, 17042 La Rochelle Cedex 1, France.
Lamare
S
S
Legoy
M-D
MD
Goubet
I
I
D002073:Butanes / Q000378:metabolism*; D004795:Enzyme Stability; D004798:Enzymes / Q000378:metabolism*; D004926:Escherichia coli / Q000201:enzymology* / Q000378:metabolism*; D006219:Halogens / Q000378:metabolism*
D002073:Butanes; D004798:Enzymes; D006219:Halogens; C006854:butyl chloride