Mesenchymal stem cells derived from canine umbilical cord vein--a novel source for cell therapy studies.
The canine model provides a large animal system to evaluate many treatment modalities using stem cells (SCs). However, only bone marrow (BM) protocols have been widely used in dogs for preclinical approaches. BM donation consists of an invasive procedure and the number and differentiation potential of its mesenchymal stem cells (MSCs) decline with age. More recently, umbilical cord was introduced as an alternative source to BM since it is obtained from a sample that is routinely discarded. Here, we describe the isolation of MSCs from canine umbilical cord vein (cUCV). These cells can be obtained from every cord received and grow successfully in culture. Their multipotent plasticity was demonstrated by their capacity to differentiate in adipocytic, chondrocytic, and osteocytic lineages. Furthermore, our results open possibilities to use cUCV cells in preclinical trials for many well-characterized canine model conditions homologs to human diseases.
Zucconi
Eder
E
Human Genome Research Center, Department of Genetic and Evolutive Biology, University of São Paulo, São Paulo, Brazil.
Vieira
Natassia M
NM
Bueno
Daniela F
DF
Secco
Mariane
M
Jazedje
Tatiana
T
Ambrosio
Carlos E
CE
Passos-Bueno
Maria Rita
MR
Miglino
Maria Angelica
MA
Zatz
Mayana
M
D017667:Adipocytes / Q000166:cytology / Q000378:metabolism; D000818:Animals; D002454:Cell Differentiation; D019070:Cell Lineage; D049109:Cell Proliferation; D002469:Cell Separation; D002478:Cells, Cultured; D019902:Chondrocytes / Q000166:cytology / Q000378:metabolism; D004285:Dogs; D005434:Flow Cytometry; D020869:Gene Expression Profiling; D007150:Immunohistochemistry; D016130:Immunophenotyping; D045164:Mesenchymal Stem Cell Transplantation / Q000379:methods*; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism; D039902:Multipotent Stem Cells / Q000166:cytology / Q000378:metabolism; D010011:Osteocytes / Q000166:cytology / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013997:Time Factors; D014471:Umbilical Veins / Q000166:cytology*