title: Endothelial nitric oxide synthase activation contributes to post-exercise hypotension in spontaneously hypertensive rats.
abstract: We investigated the role that endothelial nitric oxide synthase plays in post-exercise hypotension in spontaneously hypertensive rats. To accomplish this, rats were subjected to a single bout of dynamic exercise on a treadmill at 15 m/min for 20 min. L-nitroarginine methyl ester (L-NAME, 40 mg/kg, i.p.) significantly inhibited post-exercise hypotension (25+/-11 and 5+/-3 mm Hg, respectively; P<0.05). In addition, the superoxide anion generation was decreased, while the plasma nitrite production and serine phosphorylation of endothelial nitric oxide synthase were significantly elevated in spontaneously hypertensive rats at 30 min after the termination of exercise. Taken together, these data demonstrate that the increased phosphorylation of endothelial nitric oxide synthase plays a crucial role in the reduction of arterial pressure following a single bout of dynamic exercise in spontaneously hypertensive rats.
authors.affiliation: Infection Signaling Network Research Center, Research Institutes of Biomedical Science, Department of Physiology, College of Medicine, Chungnam National University, Jung-Gu, Daejeon, Republic of Korea.
authors.forename: Sang Ki
authors.initials: SK
authors.lastname: Lee
authors.forename: Cuk Seong
authors.initials: CS
authors.lastname: Kim
authors.forename: Hyo Shin
authors.initials: HS
authors.lastname: Kim
authors.forename: Eun Jung
authors.initials: EJ
authors.lastname: Cho
authors.forename: Hee Kyoung
authors.initials: HK
authors.lastname: Joo
authors.forename: Ji Young
authors.initials: JY
authors.lastname: Lee
authors.forename: Eun Ji
authors.initials: EJ
authors.lastname: Lee
authors.forename: Jin Bong
authors.initials: JB
authors.lastname: Park
authors.forename: Byeong Hwa
authors.initials: BH
authors.lastname: Jeon
mesh_terms: D000818:Animals; D004789:Enzyme Activation; D004791:Enzyme Inhibitors / Q000494:pharmacology; D007022:Hypotension / Q000201:enzymology*; D008297:Male; D019331:NG-Nitroarginine Methyl Ester / Q000494:pharmacology; D052250:Nitric Oxide Synthase Type III / Q000037:antagonists & inhibitors / Q000378:metabolism*; D010766:Phosphorylation; D010805:Physical Conditioning, Animal; D051381:Rats; D011918:Rats, Inbred SHR
chemical_list: D004791:Enzyme Inhibitors; D052250:Nitric Oxide Synthase Type III; D019331:NG-Nitroarginine Methyl Ester