Icariin enhances neuronal survival after oxygen and glucose deprivation by increasing SIRT1.
It has been reported that icariin protects neurons against ischemia/reperfusion injury. In this study, we found that icariin could enhance neuronal viability and suppress neuronal death after oxygen and glucose deprivation (OGD). Further study showed that neuroprotection by icariin was through the induction of Sirtuin type 1 (SIRT1), an effect that was reversed by SIRT1 inhibitor III and P38 inhibitor SB203580. SIRT1 is an endogenous gene of longevity, which increased neuronal viability and could be activated by stimulating the mitogen-activated protein kinase (MAPK) pathway. However, this study found that icariin activated the MAPK/P38 pathway, not the extracellular signal-regulated kinase (MAPK/ERK) or c-Jun N-terminal protein kinase (MAPK/JNK) to regulate SIRT1 expression. The results suggest that icariin may be developed into a neuroprotectant for ischemia-related brain injury.
Wang
Lin
L
Department of Neurology, Affiliated Drum Tower Hospital of Nanjing University Medical School, PR China.
Zhang
Ling
L
Chen
Zhi-Bin
ZB
Wu
Jia-Yong
JY
Zhang
Xin
X
Xu
Yun
Y
D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000166:cytology; D004622:Embryo, Mammalian; D005419:Flavonoids / Q000494:pharmacology*; D005562:Formazans / Q000032:analysis / Q000378:metabolism; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D005947:Glucose / Q000494:pharmacology*; D007770:L-Lactate Dehydrogenase / Q000032:analysis / Q000378:metabolism; D051379:Mice; D009474:Neurons; D018696:Neuroprotective Agents / Q000494:pharmacology*; D010100:Oxygen / Q000494:pharmacology*; D056564:Sirtuin 1; D037761:Sirtuins / Q000235:genetics / Q000378:metabolism*; D013696:Temperature; D013778:Tetrazolium Salts / Q000032:analysis / Q000378:metabolism; D013997:Time Factors
D005419:Flavonoids; D005562:Formazans; D018696:Neuroprotective Agents; D013778:Tetrazolium Salts; C079782:MTT formazan; D007770:L-Lactate Dehydrogenase; C470545:Sirt1 protein, mouse; D056564:Sirtuin 1; D037761:Sirtuins; D005947:Glucose; D010100:Oxygen; C056599:icariin