title: HBx protein induces EMT through c-Src activation in SMMC-7721 hepatoma cell line.
abstract: The relationships between epithelial-to-mesenchymal transition (EMT), hepatitis B virus X protein (HBx), and the non-receptor tyrosine kinase c-Src were investigated. The HBx gene transfected SMMC-7721 cells underwent morphological changes from a classic epithelial morphology to a spindle-like shape. The HBx transfection increased the invasive potential of these cells. When the transfected cells were exposed to the c-Src kinase inhibitor PP2, the cells recovered their original epithelial morphology and the mRNA and protein expression of epithelial and mesenchymal markers returned to the parental cell levels. Our data suggest that activated c-Src played a critical role in the HBx-induced EMT of SMMC-7721 cells.
authors.affiliation: Department of Hepatobiliary Surgery, Southwest Hospital, Third Military Medical University, ChongQing 400038, China.
authors.forename: Shi Zhong
authors.initials: SZ
authors.lastname: Yang
authors.forename: Lei Da
authors.initials: LD
authors.lastname: Zhang
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhang
authors.forename: Yan
authors.initials: Y
authors.lastname: Xiong
authors.forename: Yu Jun
authors.initials: YJ
authors.lastname: Zhang
authors.forename: Hao Lin
authors.initials: HL
authors.lastname: Li
authors.forename: Xiao Wu
authors.initials: XW
authors.lastname: Li
authors.forename: Jia Hong
authors.initials: JH
authors.lastname: Dong
mesh_terms: D006528:Carcinoma, Hepatocellular / Q000201:enzymology / Q000473:pathology* / Q000821:virology; D045744:Cell Line, Tumor; D004848:Epithelium / Q000201:enzymology / Q000473:pathology / Q000821:virology; D006515:Hepatitis B virus / Q000378:metabolism*; D006801:Humans; D008113:Liver Neoplasms / Q000201:enzymology / Q000473:pathology* / Q000821:virology; D008648:Mesoderm / Q000201:enzymology / Q000473:pathology / Q000821:virology; D009361:Neoplasm Invasiveness; D047428:Protein Kinase Inhibitors / Q000494:pharmacology; D011743:Pyrimidines / Q000494:pharmacology; D015534:Trans-Activators / Q000235:genetics / Q000378:metabolism*; D054334:Viral Regulatory and Accessory Proteins / Q000235:genetics / Q000378:metabolism*; D019061:src-Family Kinases / Q000378:metabolism*
chemical_list: C412373:AG 1879; D047428:Protein Kinase Inhibitors; D011743:Pyrimidines; D015534:Trans-Activators; D054334:Viral Regulatory and Accessory Proteins; C057628:hepatitis B virus X protein; D019061:src-Family Kinases