title: [Cloning and eukaryotic expression of the gene encoding myosin from Brugia malayi].
abstract: Total RNA was extracted from periodic microfilariae of Brugia malayi and its myosin partial gene (Bm-M55) was amplified by RT-PCR. The PCR product was cloned and then subcloned into pcDNA3.1 (+)vector. The recombinant eukaryotic plasmids were screened and identified by digestion with restriction enzyme and PCR amplification, and was transfected into COS-7 cells subsequently. The expressed protein was identified by SDS-PAGE. Bm-M55 mRNA was highly expressed in transfected COS-7 cells. The deduced amino acid sequence showed to be identical with that of Bm-M55, and the recombinant protein was about Mr 55000.
authors.affiliation: Department of Parasitology, School of Basic Medical Sciences, Nantong University, Nantong 226001, China.
authors.forename: Dong-fang
authors.initials: DF
authors.lastname: Xie
authors.forename: Zheng
authors.initials: Z
authors.lastname: Fang
authors.forename: Wei-qun
authors.initials: WQ
authors.lastname: Huang
authors.forename: Qin
authors.initials: Q
authors.lastname: Shen
authors.forename: Hai-yan
authors.initials: HY
authors.lastname: Tong
authors.forename: Bang-sheng
authors.initials: BS
authors.lastname: Xu
mesh_terms: D000818:Animals; D017178:Brugia malayi / Q000235:genetics* / Q000378:metabolism; D019556:COS Cells; D002522:Chlorocebus aethiops; D003001:Cloning, Molecular; D015870:Gene Expression; D017238:Genes, Helminth; D005822:Genetic Vectors; D009218:Myosins / Q000235:genetics* / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014162:Transfection
chemical_list: D009218:Myosins