title: Development of a sensitive enzyme-linked immunosorbent assay for measurement of DNase I in human serum.
abstract: BACKGROUND
  Serum deoxyribonuclease I (DNase I) activity was reported to increase in the early phase after onset of acute myocardial infarction (AMI). Up to now, DNase I activity has been quantified by the single radial enzyme diffusion (SRED) method, which unfortunately requires a long incubation time. Therefore it is necessary to develop another assay suitable for measurement of serum DNase I concentrations in a clinical setting.
  
  
  METHODS
  A sandwich ELISA was established for measurement of DNase I protein using a polyclonal antibody directed against DNase I protein and a biotinylated monoclonal for subsequent detection. Concentrations of serum DNase I protein were measured in healthy individuals and patients with AMI.
  
  
  RESULTS
  This method was as precise as SRED, and took less time than SRED. A significant correlation was observed between DNase I concentration and enzyme activity (r=0.839; P<0.001). The average of serum DNase I in AMI patients within 0-12 h of chest pain was significantly higher than that in healthy individuals (P<0.001), and decreased with time.
  
  
  CONCLUSIONS
  We have developed a sensitive ELISA capable of measuring DNase I protein concentrations. This method may be a useful alternative to SRED as an aid to diagnosis of AMI based on the serum DNase I level.
authors.affiliation: Department of Legal Medicine, Gunma University, Graduate School of Medicine, Gunma, Japan.
authors.forename: Tamiko
authors.initials: T
authors.lastname: Nakajima
authors.forename: Rie
authors.initials: R
authors.lastname: Takagi
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Tajima
authors.forename: Chikako
authors.initials: C
authors.lastname: Makita
authors.forename: Yoshihiko
authors.initials: Y
authors.lastname: Kominato
authors.forename: Jun
authors.initials: J
authors.lastname: Kuribara
authors.forename: Shigeru
authors.initials: S
authors.lastname: Ohshima
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Tada
authors.forename: Hideki
authors.initials: H
authors.lastname: Tsurugaya
authors.forename: Yasuyuki
authors.initials: Y
authors.lastname: Kobayashi
authors.forename: Haruo
authors.initials: H
authors.lastname: Takeshita
authors.forename: Yasuyuki
authors.initials: Y
authors.lastname: Kawai
authors.forename: Toshihiro
authors.initials: T
authors.lastname: Yasuda
mesh_terms: D000328:Adult; D000906:Antibodies / Q000276:immunology; D000918:Antibody Specificity; D003850:Deoxyribonuclease I / Q000097:blood* / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009203:Myocardial Infarction / Q000097:blood / Q000201:enzymology; D012680:Sensitivity and Specificity; D013997:Time Factors
chemical_list: D000906:Antibodies; D003850:Deoxyribonuclease I