Engraftment of human blood malignancies to the turkey embryo: a robust new in vivo model.
Xenografting of human blood malignancies to immunodeficient SCID mice is a powerful research tool. We evaluate here whether the immunodeficient turkey embryo can also serve as a xenograft host for human blood malignancies. Human leukemia, lymphoma and myeloma lines engrafted robustly into medullary and extramedullary tissues of turkey embryos as detected by PCR, FACS and histology in 8-10 days. Four of eleven patient AML samples also engrafted the bone marrow. Grafts of two lines responded to chemotherapy with doxorubicin. The turkey embryo therefore has the potential to be a complementary xenograft model for the study of human blood malignancies.
Grinberg
Igor
I
Mina and Everard Goodman Faculty of Life Sciences, Bar-Ilan University, Ramat-Gan, Israel.
Reis
Arbel
A
Ohana
Avivit
A
Taizi
Moran
M
Cipok
Michal
M
Tavor
Sigal
S
Rund
Deborah
D
Deutsch
Varda R
VR
Goldstein
Ronald S
RS
D000818:Animals; D001803:Blood Transfusion / Q000379:methods; D045744:Cell Line, Tumor / Q000637:transplantation*; D002642:Chick Embryo; D002645:Chickens; D017931:DNA Primers; D006801:Humans; D007231:Infant, Newborn; D007938:Leukemia; D008223:Lymphoma; D009101:Multiple Myeloma; D009368:Neoplasm Transplantation / Q000276:immunology / Q000379:methods*; D016133:Polymerase Chain Reaction; D013045:Species Specificity; D014183:Transplantation, Heterologous / Q000276:immunology / Q000379:methods; D014422:Turkeys / Q000196:embryology*
D017931:DNA Primers