Activation of mitogen-activated protein kinases is involved in the induction of interferon beta gene in macrophages infected with Orientia tsutsugamushi.
We investigated the role of MAPK in IFN-beta gene expression in macrophages after infection with Orientia tsutsugamushi. ERK1/2 became phosphorylated in Orientia-stimulated macrophages. Selective inhibition of ERK1/2 and p38 MAPK could all significantly reduce Orientia-stimulated IFN-beta mRNA expression. Orientia inactivation by heat abolished IFN-beta mRNA induction only, whereas cytochalasin D treatment completely blocked both IFN-beta and chemokine expression, suggesting requirement of cellular internalization by viable bacteria for IFN-beta gene induction. In conclusion, our data indicate that MAPK pathways are required to induce maximal IFN-beta gene expression in macrophages during Orientia infection.
Koo
Jung-Eun
JE
Department of Microbiology, School of Medicine and Institute of Medical Science, Cheju National University, Jeju, Republic of Korea.
Yun
Ji-Hyun
JH
Lee
Keun-Hwa
KH
Hyun
Jin-Won
JW
Kang
Hee-Kyoung
HK
Jang
Won-Jong
WJ
Park
Kyung-Hee
KH
Koh
Young-Sang
YS
D000818:Animals; D002460:Cell Line; D015638:Cytochalasin D / Q000494:pharmacology; D005786:Gene Expression Regulation; D006358:Hot Temperature; D016899:Interferon-beta / Q000235:genetics / Q000378:metabolism*; D008264:Macrophages / Q000378:metabolism* / Q000382:microbiology; D051379:Mice; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism*; D012285:Orientia tsutsugamushi / Q000187:drug effects / Q000472:pathogenicity*; D010766:Phosphorylation; D012333:RNA, Messenger / Q000378:metabolism; D015398:Signal Transduction; D013997:Time Factors
D012333:RNA, Messenger; D015638:Cytochalasin D; D016899:Interferon-beta; D020928:Mitogen-Activated Protein Kinases