Inhibitory effects of constituents of Morinda citrifolia seeds on elastase and tyrosinase.
A 50% ethanolic extract (MCS-ext) from seeds of Morinda citrifolia ("noni" seeds) showed more potent in vitro inhibition of elastase and tyrosinase, and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity than extracts of M. citrifolia leaves or flesh. Activity-guided fractionation of MCS-ext using in vitro assays led to the isolation of ursolic acid as an active constituent of elastase inhibitory activity. 3,3'-Bisdemethylpinoresinol, americanin A, and quercetin were isolated as active constituents having both tyrosinase inhibitory and radical scavenging activities. Americanin A and quercetin also showed superoxide dismutase (SOD)-like activity. These active compounds were isolated from noni seeds for the first time.
Masuda
Megumi
M
School of Pharmacy, Kinki University, 3-4-1 Kowakae, Higashiosaka, Osaka, 577-8502, Japan.
Murata
Kazuya
K
Fukuhama
Akiko
A
Naruto
Shunsuke
S
Fujita
Tadashi
T
Uwaya
Akemi
A
Isami
Fumiyuki
F
Matsuda
Hideaki
H
D004789:Enzyme Activation / Q000187:drug effects; D016166:Free Radical Scavengers / Q000302:isolation & purification / Q000494:pharmacology; D014442:Monophenol Monooxygenase / Q000378:metabolism*; D032066:Morinda / Q000737:chemistry*; D010196:Pancreatic Elastase / Q000378:metabolism*; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry; D012639:Seeds / Q000737:chemistry*
D016166:Free Radical Scavengers; D010936:Plant Extracts; D014442:Monophenol Monooxygenase; D010196:Pancreatic Elastase