Identification of tumor-associated antigens by using SEREX in hepatocellular carcinoma.
OBJECTIVE
To identify biomarkers for diagnosis and prognosis of hepatocellular carcinoma (HCC).


METHODS
Screening the HCC cDNA library with HCC patients sera. Isolated proteins were used as antigens to detect antibodies from patients with HCC and control sera.


RESULTS
Eighty-one positive clones were identified. The frequencies of autoantibody against five HCC-associated antigens were higher in HCC than that in chronic hepatitis and normal human sera. The sensitivity and specificity of KRT23, AHSG and FTL antigens combination tests up to 98.2% in joint test and 90.0% in series test separately.


CONCLUSIONS
HCC associate antigens identified from this study supply candidate markers of diagnosis, combined detection and immunotherapy of HCC.
Wang
Kaijuan
K
Department of Epidemiology, Zhengzhou University, China.
Xu
Xueqin
X
Nie
Yifei
Y
Dai
Liping
L
Wang
Peng
P
Zhang
Jianying
J
D000595:Amino Acid Sequence; D000951:Antigens, Neoplasm / Q000235:genetics*; D001323:Autoantibodies / Q000097:blood; D014408:Biomarkers, Tumor / Q000032:analysis*; D006528:Carcinoma, Hepatocellular / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D015723:Gene Library; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D008969:Molecular Sequence Data; D012680:Sensitivity and Specificity
D000951:Antigens, Neoplasm; D001323:Autoantibodies; D014408:Biomarkers, Tumor