title: SUnSET, a nonradioactive method to monitor protein synthesis.
abstract: We developed a nonradioactive fluorescence-activated cell sorting-based assay, called surface sensing of translation (SUnSET), which allows the monitoring and quantification of global protein synthesis in individual mammalian cells and in heterogeneous cell populations. We demonstrate here, using mouse dendritic and T cells as a model, that SUnSET offers a technical alternative to classical radioactive labeling methods for the study of mRNA translation and cellular activation.
authors.affiliation: Centre d'Immunologie de Marseille-Luminy, Université de la Méditerranée, France.
authors.forename: Enrico K
authors.initials: EK
authors.lastname: Schmidt
authors.forename: Giovanna
authors.initials: G
authors.lastname: Clavarino
authors.forename: Maurizio
authors.initials: M
authors.lastname: Ceppi
authors.forename: Philippe
authors.initials: P
authors.lastname: Pierre
mesh_terms: D020869:Gene Expression Profiling / Q000379:methods*; D015151:Immunoblotting / Q000379:methods*; D007553:Isotope Labeling; D014176:Protein Biosynthesis / Q000502:physiology*; D011506:Proteins / Q000032:analysis* / Q000378:metabolism*; D011691:Puromycin / Q000032:analysis*; D013194:Staining and Labeling / Q000379:methods
chemical_list: D011506:Proteins; D011691:Puromycin