title: Generation of transgenic mice.
abstract: This unit describes detailed step-by-step protocols, reagents, and equipment required for successful generation of transgenic mice using pronuclear injection. The experimental methods and practical tips given here will help guide beginners in understanding what is required and what to avoid in these standard protocols for efficiently generating transgenic mice.
authors.affiliation: National Institute of Dental and Craniofacial Research, National Institutes of Health, Department of Health and Human Services, Bethesda, Maryland, USA.
authors.forename: Andrew
authors.initials: A
authors.lastname: Cho
authors.forename: Naoto
authors.initials: N
authors.lastname: Haruyama
authors.forename: Ashok B
authors.initials: AB
authors.lastname: Kulkarni
mesh_terms: D000818:Animals; D010064:Embryo Implantation; D005818:Genetic Engineering / Q000379:methods*; D005838:Genotype; D051379:Mice; D008822:Mice, Transgenic; D053652:Nuclear Transfer Techniques; D019076:Transgenes