Direct detection of plant viruses in potato tubers using real-time PCR.
Virus indexing of seed potatoes can be carried out by growing eye plugs to produce small plants and then testing them by ELISA, but this method is time consuming. Direct testing of the eye plugs by ELISA is not reliable, and so a method has been developed for the routine testing of seed potatoes for virus by PCR.
Boonham
Neil
N
Central Science Laboratories, Sand Hutton, York, UK.
Laurenson
Lynn
L
Weekes
Rebecca
R
Mumford
Rick
R
D019411:Clinical Laboratory Techniques; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D021141:Nucleic Acid Amplification Techniques; D010942:Plant Viruses / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012367:RNA, Viral / Q000032:analysis / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012680:Sensitivity and Specificity; D011198:Solanum tuberosum / Q000821:virology*
D012367:RNA, Viral