title: [Expression of special AT-rich sequence-binding protein mRNA and its clinicopathological significance in non-small cell lung cancer].
abstract: OBJECTIVE
  To detect the expression of AT-rich sequence-binding protein (SATB1) mRNA in non-small cell lung cancer (NSCLC) and explore the role of SATB1 in the development of NSCLC.
  
  
  METHODS
  The total RNA was extracted from NSCLC tissues and normal lung tissues and reverse transcribed into cDNA. Real-time fluorescence quantitative RT-PCR was performed for detecting the expression of SATB1 mRNA these tissues.
  
  
  RESULTS
  The expression of SATB1 mRNA was 13-fold higher in NSCLC tissues than in normal lung tissues (P<0.001), and in metastatic and nonmetastatic NSCLC, the expression was 23.63 and 5.57 folds that in normal lung tissues, respectively.
  
  
  CONCLUSIONS
  SATB1 mRNA expression might be associated with the development and lymph node metastasis of NSCLC and may potentially used as an indicator for predicting the prognosis of NSCLC.
authors.affiliation: Department of Cardiothoracic Surgery, Zhujiang Hospital, Southern Medical University, Guangzhou, China. zhoulaiyong@yahoo.com.cn
authors.forename: Lai-Yong
authors.initials: LY
authors.lastname: Zhou
authors.forename: Fang
authors.initials: F
authors.lastname: Liu
authors.forename: Jian
authors.initials: J
authors.lastname: Tong
authors.forename: Qun-Qing
authors.initials: QQ
authors.lastname: Chen
authors.forename: Fu-Wei
authors.initials: FW
authors.lastname: Zhang
mesh_terms: D000328:Adult; D000368:Aged; D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D005260:Female; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D008207:Lymphatic Metastasis; D008297:Male; D036961:Matrix Attachment Region Binding Proteins / Q000235:genetics / Q000378:metabolism*; D008875:Middle Aged; D011379:Prognosis; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods
chemical_list: D036961:Matrix Attachment Region Binding Proteins; D012333:RNA, Messenger; C076189:SATB1 protein, human