title: 2-(1-Hydroxethyl)-4,8-dihydrobenzo[1,2-b:5,4-b']dithiophene-4,8-dione (BTP-11) enhances the ATRA-induced differentiation in human leukemia HL-60 cells.
abstract: 2-(1-Hydroxethyl)-4,8-dihydrobenzo[1,2-b:5,4-b']dithiophene-4,8-dione (BTP-11) is a potent enhancer for all-trans retinoic acid (ATRA)-induced differentiation in HL-60 cells. Combination of BTP-11 and ATRA cut down the concentration of ATRA significantly, and that BTP-11 promoted the progression of ATRA-induced into the terminal granulocytic differentiation. Further, Western blot analysis revealed that combination of BTP-11 and ATRA decreased cyclin D/CDK4 and increased C/EBPvarepsilon protein expression to arrest the cells into G0/G1 phase leading to granulocytic maturation. These results confirmed that BTP-11 is a potent enhancer for ATRA-induced differentiation of HL-60 cells, and the great developmental potential of BTP-11 will be expected.
authors.affiliation: Graduate Institute of Pharmaceutical Chemistry, China Medical University, Taichung, Taiwan.
authors.forename: Chun-Jen
authors.initials: CJ
authors.lastname: Chen
authors.forename: Yen-Fang
authors.initials: YF
authors.lastname: Wen
authors.forename: Pi-Tsan
authors.initials: PT
authors.lastname: Huang
authors.forename: Mei-Hua
authors.initials: MH
authors.lastname: Hsu
authors.forename: Kuo-Hsiung
authors.initials: KH
authors.lastname: Lee
authors.forename: Mei-Hwai
authors.initials: MH
authors.lastname: Chen
authors.forename: Wuu-Chian
authors.initials: WC
authors.lastname: Shin
authors.forename: Li-Jiau
authors.initials: LJ
authors.lastname: Huang
authors.forename: Sheng-Chu
authors.initials: SC
authors.lastname: Kuo
mesh_terms: D015703:Antigens, CD / Q000276:immunology; D015153:Blotting, Western; D002454:Cell Differentiation / Q000187:drug effects*; D004357:Drug Synergism; D005434:Flow Cytometry; D016193:G1 Phase; D018922:HL-60 Cells; D006801:Humans; D016192:Resting Phase, Cell Cycle; D013876:Thiophenes / Q000494:pharmacology*; D014212:Tretinoin / Q000494:pharmacology*
chemical_list: C544856:2-(1-hydroxethyl)-4,8-dihydrobenzo(1,2-b:5,4-b')dithiophene-4,8-dione; D015703:Antigens, CD; D013876:Thiophenes; D014212:Tretinoin