Preclinical models of HPV+ and HPV- HNSCC in mice: an immune clearance of HPV+ HNSCC.
BACKGROUND
To investigate whether human papilloma virus (HPV)-specific immune mechanisms can result in tumor clearance, we have created HPV+ and HPV- tonsil cells that form squamous cancers in immune-competent mice. Here, we determine that an immune-specific response can clear HPV+ tumor cells and the cellular requirements to mediate this tumor clearance.


METHODS
Through the benefit of this model, we use in vitro and in vivo methods to better understand how HPV+ cells are rejected. RESULT.: The data show that an in vivo antigen-specific antitumor response is generated to HPV+ transformed cells and that this response requires CD4+ and CD8+ cells to mount this antitumor response.


CONCLUSIONS
The findings from this preclinical model will have implications not only in understanding human disease but also as a valuable model for testing immunotherapeutic strategies for HPV+ head and neck cancer.
Williams
Robin
R
Department of Otolaryngology, University of Iowa, Iowa City Veterans Administration Medical Center, Iowa City, Iowa, USA.
Lee
Dong Wook
DW
Elzey
Bennett D
BD
Anderson
Mary E
ME
Hostager
Bruce S
BS
Lee
John H
JH
D019264:Adoptive Transfer; D000818:Animals; D000956:Antigens, Viral / Q000502:physiology*; D015496:CD4-Positive T-Lymphocytes; D018414:CD8-Positive T-Lymphocytes; D002294:Carcinoma, Squamous Cell / Q000276:immunology* / Q000473:pathology* / Q000821:virology; D004195:Disease Models, Animal; D006258:Head and Neck Neoplasms / Q000276:immunology* / Q000473:pathology* / Q000821:virology; D052162:Human papillomavirus 16 / Q000276:immunology*; D051379:Mice; D008810:Mice, Inbred C57BL; D047368:Tumor Burden
D000956:Antigens, Viral