Enzyme-enhanced fluorescence detection of DNA on etched optical fibers.
A novel DNA biosensor based on enzyme-enhanced fluorescence detection on etched optical fibers was developed. The hybridization complex of DNA probe and biotinylated target was formed on the etched optical fiber, and was then bound with streptavidin labeled horseradish peroxidase (streptavidin-HRP). The target DNA was quantified through the fluorescent detection of bi-p,p'-4-hydroxyphenylacetic acid (DBDA) generated from the substrate 4-hydroxyphenylacetic acid (p-HPA) under the catalysis of HRP, with a detection limit of 1 pM and a linear range from 1.69 pM to 169 pM. It is facile to regenerate this sensor through surface treatment with concentrated urea solution. It was discovered that the sensor can retain 70% of its original activity after three detection-regeneration cycles.
Niu
Shu-yan
SY
Key Lab of Eco-chemical Engineering, Ministry of Education, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, PR China.
Li
Quan-yi
QY
Ren
Rui
R
Zhang
Shu-sheng
SS
D019840:2-Propanol / Q000737:chemistry; D001426:Bacterial Proteins / Q000378:metabolism*; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D019921:Biotinylation; D004247:DNA / Q000032:analysis*; D015342:DNA Probes / Q000737:chemistry*; D005453:Fluorescence; D006735:Horseradish Peroxidase / Q000378:metabolism*; D009693:Nucleic Acid Hybridization; D055100:Optical Fibers; D010648:Phenylacetates / Q000032:analysis / Q000378:metabolism*; D012680:Sensitivity and Specificity; D013997:Time Factors
D001426:Bacterial Proteins; D015342:DNA Probes; D010648:Phenylacetates; C058865:Strep-avidin conjugated horseradish peroxidase; C008070:4-hydroxyphenylacetic acid; D004247:DNA; D006735:Horseradish Peroxidase; D019840:2-Propanol