Secreted TARSH regulates olfactory mitral cell dendritic complexity.
Olfactory sensory neurons synapse with mitral cells to form stereotyped connections in the olfactory bulb (OB). Mitral cell apical dendrites receive input from olfactory sensory neurons expressing the same odorant receptor. During development, this restricted dendritic targeting of mitral cells is achieved through eliminating elaborated dendritic trees to a single apical dendrite. Through a genome-wide microarray screen, we identified TARSH (Target of NESH SH3) as a transiently expressed molecule in mitral cells during the dendritic refinement period. TARSH expression is restricted to pyramidal neurons along the main olfactory pathway, including the anterior olfactory nucleus and piriform cortex. The dynamic TARSH expression is not altered when odor-evoked activity is blocked by naris closure or in AC3 knockout mice. We also demonstrate that TARSH is a secreted protein. In dissociated OB cultures, secreted TARSH promotes the reduction of mitral cell dendritic complexity and restricts dendritic branching and outgrowth of interneurons. Dendritic morphological changes were also observed in mitral cells overexpressing TARSH themselves. We propose that TARSH is part of the genetic program that regulates mitral cell dendritic refinement.
Cheng
Ting-Wen
TW
Department of Cell Biology and Human Anatomy, School of Medicine, University of California, Davis, CA 95616, USA.
Gong
Qizhi
Q
D000367:Age Factors; D000818:Animals; D000831:Animals, Newborn; D019556:COS Cells; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism* / Q000494:pharmacology; D002478:Cells, Cultured; D002522:Chlorocebus aethiops; D003712:Dendrites / Q000187:drug effects / Q000502:physiology*; D004622:Embryo, Mammalian; D018507:Gene Expression Regulation, Developmental / Q000502:physiology*; D007211:Indoles / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D046228:Microarray Analysis / Q000379:methods; D009415:Nerve Net / Q000166:cytology / Q000502:physiology; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism; D017711:Nonlinear Dynamics; D009812:Odorants; D009830:Olfactory Bulb / Q000166:cytology* / Q000254:growth & development / Q000378:metabolism; D009833:Olfactory Pathways / Q000378:metabolism; D011984:Sensory Receptor Cells / Q000145:classification / Q000166:cytology* / Q000378:metabolism; D013997:Time Factors; D014162:Transfection
D002352:Carrier Proteins; D007211:Indoles; D009419:Nerve Tissue Proteins; C527603:TARSH protein, mouse; C007293:DAPI