Visualization of plastids in pollen grains: involvement of FtsZ1 in pollen plastid division.
Visualizing organelles in living cells is a powerful method to analyze their intrinsic mechanisms. Easy observation of chlorophyll facilitates the study of the underlying mechanisms in chloroplasts, but not in other plastid types. Here, we constructed a transgenic plant enabling visualization of plastids in pollen grains. Combination of a plastid-targeted fluorescent protein with a pollen-specific promoter allowed us to observe the precise number, size and morphology of plastids in pollen grains of the wild type and the ftsZ1 mutant, whose responsible gene plays a central role in chloroplast division. The transgenic material presented in this work is useful for studying the division mechanism of pollen plastids.
Tang
Lay Yin
LY
Research Institute for Bioresources, Okayama University, Kurashiki, Okayama 710-0046, Japan.
Nagata
Noriko
N
Matsushima
Ryo
R
Chen
Yuling
Y
Yoshioka
Yasushi
Y
Sakamoto
Wataru
W
D017360:Arabidopsis / Q000166:cytology / Q000235:genetics* / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D008854:Microscopy, Electron; D008856:Microscopy, Fluorescence; D016254:Mutagenesis, Insertional; D030821:Plants, Genetically Modified / Q000235:genetics / Q000378:metabolism; D018087:Plastids / Q000235:genetics / Q000378:metabolism / Q000648:ultrastructure*; D011058:Pollen / Q000166:cytology* / Q000378:metabolism
D029681:Arabidopsis Proteins; C523306:FtsZ1 protein, Arabidopsis; D049452:Green Fluorescent Proteins