Determination of tectoridin in rat plasma by high-performance liquid chromatography and its application to pharmacokinetic studies.
A simple, specific and sensitive HPLC method with UV detection was developed and validated for the determination of tectoridin in rat plasma for the first time. Chromatographic separation was performed on a Welchrom C(18) column (150 x 4.6 mm, i.d., 5 microm) at a flow rate of 1.0 mL min(-1), using a mixture of methanol-2% HAc aqueous solution (31:69, v/v) as the mobile phase with UV detection at 266 nm. The calibration curves for tectoridin were linear over the concentration range of 1.10-274.40 microg mL(-1) in rat plasma. The intra- and inter-day accuracies (RE) were within -3.23% and 4.11%. The intra- and inter-day precisions (RSD) were not more than 2.74 and 4.72%, respectively. The present method was successfully applied to the pharmacokinetic studies of tectoridin in rats after intravenous administration of three different doses.
Liu
Taiming
T
Key Laboratory of Drug Targeting, West China School of Pharmacy, Sichuan University, Chengdu, People's Republic of China.
Chen
Zhuo
Z
Guo
Yuyi
Y
Jiang
Xuehua
X
Ma
Guo
G
D000818:Animals; D019540:Area Under Curve; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004355:Drug Stability; D007529:Isoflavones / Q000097:blood* / Q000493:pharmacokinetics; D016018:Least-Squares Analysis; D051381:Rats; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D007529:Isoflavones; C120040:tectoridin