Antihyperglycemic effects of fruits of privet (Ligustrum obtusifolium) in streptozotocin-induced diabetic rats fed a high fat diet.
The protective effects of freeze-dried privet (Ligustrum obtusifolium) fruits (PFs) were observed in streptozotocin (STZ)-induced diabetic rats on a high fat diet by measuring levels of blood glucose, serum insulin, fructosamine, and hepatic reactive oxygen species generating and scavenging enzyme activities. A PF-supplemented diet was prepared by mixing an AIN-76 diet with powdered PF (final concentration, 1% or 2%). It was fed to STZ-induced diabetic rats on a high fat diet for 6 weeks. Diabetic animals receiving the PF-supplemented diet showed a significant increase in body weight, feed efficiency ratio, liver, kidney, and heart weight, and serum glucose, insulin, and fructosamine levels compared with high fat diet-fed diabetic animals. The treatment with PF showed improved hepatic glutathione S-transferase, superoxide dismutase, and xanthine oxidase activities as well as glutathione and lipid peroxide levels in the diabetic animals. Intracellular swelling and vacuole formation in diabetic pancreatic beta- and delta-cells were ameliorated by the PF-supplemented diet. Furthermore, necrosis of tubular epithelial cells and dilatation of luminal space in diabetic kidneys exhibited near-noninjured condition. This is the first time an antihyperglycemic effect of L. obtusifolium fruit in STZ-induced diabetic rats has been identified.
Lee
Sang-Il
SI
Department of Food, Nutrition and Cookery, Keimyung College, Daegu, Republic of Korea.
Oh
Sung-Hee
SH
Park
Kun-Young
KY
Park
Bum-Ho
BH
Kim
Jeong-Sook
JS
Kim
Soon-Dong
SD
D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D015415:Biomarkers / Q000097:blood; D001786:Blood Glucose; D003921:Diabetes Mellitus, Experimental / Q000097:blood / Q000178:diet therapy*; D004041:Dietary Fats; D019270:Fructosamine / Q000097:blood; D005638:Fruit; D005978:Glutathione / Q000097:blood; D005982:Glutathione Transferase / Q000097:blood; D007004:Hypoglycemic Agents / Q000494:pharmacology / Q000627:therapeutic use*; D007328:Insulin / Q000097:blood; D007668:Kidney / Q000187:drug effects; D031659:Ligustrum; D008297:Male; D009929:Organ Size / Q000187:drug effects; D010179:Pancreas / Q000187:drug effects; D008517:Phytotherapy; D028321:Plant Preparations / Q000494:pharmacology / Q000627:therapeutic use*; D051381:Rats; D017207:Rats, Sprague-Dawley; D017382:Reactive Oxygen Species / Q000378:metabolism; D013482:Superoxide Dismutase / Q000097:blood; D015430:Weight Gain / Q000187:drug effects; D014969:Xanthine Oxidase / Q000097:blood
D015415:Biomarkers; D001786:Blood Glucose; D004041:Dietary Fats; D007004:Hypoglycemic Agents; D007328:Insulin; D028321:Plant Preparations; D017382:Reactive Oxygen Species; D019270:Fructosamine; D013482:Superoxide Dismutase; D014969:Xanthine Oxidase; D005982:Glutathione Transferase; D000410:Alanine Transaminase; D005978:Glutathione