Simultaneous quantitation of dicaffeoylquinic acids in rat plasma after an intravenous administration of mailuoning injection using liquid chromatography-mass spectrometry.
A high-performance liquid chromatography electrospray ionization mass spectrometry method is developed and validated for the simultaneous quantitation of three major phenolic acids including 1,5-dicaffeoylquinic acid (1,5-DCQA), 3,4-dicaffeoylquinic acid (3,4-DCQA), and 3,5-dicaffeoylquinic acid (3,5-DCQA) in rat plasma. All analytes and internal standard (bergeninum) are extracted from plasma samples by liquid-liquid extraction with isopropanol. The chromatographic separation is accomplished on a stainless-steel column with a gradient 0.1% formic acid-acetonitrile solution as mobile phase at a flow rate of 0.2 mL/min with an operating temperature of 40 degrees C. The selected ion monitoring is performed at m/z 515.2 for 1,5-DCQA, 3,4-DCQA, and 3,5-DCQA, and m/z 327 for the internal standard bergeninum. Linear detection responses are obtained at a concentration range from 0.020 to 5.0 microg/mL for 1,5-DCQA, and 0.039 to 10.0 microg/mL for 3,4-DCQA and 3,5-DCQA. The lower limits of quantitation for 1,5-DCQA, 3,4-DCQA, and 3,5-DCQA are 20, 39, and 39 ng/mL, respectively. The intra- and inter-day precisions (RSD%) are within 11.0%, and the deviations of the assay accuracies are within +/- 12.0% for all analytes. The recoveries are greater than 84.0%. All analytes are proved to be stable during the sample preparation and analytic procedures. The method is successfully applied to the pharmacokinetic study of 1,5-DCQA, 3,4-DCQA, and 3,5-DCQA following an intravenous dose of 10 mL/kg mailuoning injection to rats.
Wang
Wei
W
Key Laboratory of Pharmacokinetics, China Pharmaceutical University, 1 Shennong Road, Nanjing 210038, PR China.
Hao
Hai Ping
HP
Wang
Guang Ji
GJ
Cui
Nan
N
Zheng
Chao Nan
CN
Wang
Yu Xing
YX
D000818:Animals; D002853:Chromatography, Liquid / Q000379:methods*; D004355:Drug Stability; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage* / Q000493:pharmacokinetics*; D011786:Quality Control; D011801:Quinic Acid / Q000031:analogs & derivatives* / Q000097:blood; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
D004365:Drugs, Chinese Herbal; C472707:caffeoylquinic acid; C534919:mailuoning; D011801:Quinic Acid