title: Immunohistochemical detection of the Raf kinase inhibitor protein in nonneoplastic gastric tissue and gastric cancer tissue.
abstract: Expression of the Raf kinase inhibitor protein (RKIP), a metastasis suppressor, is high in normal tissues, low in primary cancers, and lowest or absent in metastatic cancers. Here, we studied the expression of RKIP in nonneoplastic gastric tissue and gastric cancer tissue by immunohistochemistry (IHC) and evaluated its role in the genesis and metastasis of gastric cancer. RKIP immunoreactivity was evaluated in 40 samples of nonneoplastic gastric tissues and 75 samples of gastric cancer tissues. Among the 40 samples of nonneoplastic gastric tissue, 35 (87.5%) were positive for RKIP expression and 5 (12.5%) were negative; in the 75 samples of primary gastric cancer tissue, 39 (52%) were positive for RKIP expression and 36 (48%) were negative. Among 26 samples of metastatic lymph node tissues, 5 (19.2%) were positive for RKIP expression and 21 (80.8%) were negative. RKIP expression level was highest in nonneoplastic gastric tissue, low in primary gastric cancer tissue, and lowest or undetectable in metastatic gastric cancer tissue. Our data suggest that RKIP may play a role in the genesis and metastasis of gastric cancer.
authors.affiliation: Department of Surgery, LuZhou Medical College, LuZhou, Sichuan, People's Republic of China.
authors.forename: Jin
authors.initials: J
authors.lastname: Wang
authors.forename: Yong-Hong
authors.initials: YH
authors.lastname: Yang
authors.forename: An-Qun
authors.initials: AQ
authors.lastname: Wang
authors.forename: Bing
authors.initials: B
authors.lastname: Yao
authors.forename: Gang
authors.initials: G
authors.lastname: Xie
authors.forename: Gang
authors.initials: G
authors.lastname: Feng
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhang
authors.forename: Zong-Shu
authors.initials: ZS
authors.lastname: Cheng
authors.forename: Li
authors.initials: L
authors.lastname: Hui
authors.forename: Tang-Zhi
authors.initials: TZ
authors.lastname: Dai
authors.forename: Xiao-Bo
authors.initials: XB
authors.lastname: Du
authors.forename: Dong
authors.initials: D
authors.lastname: Wang
mesh_terms: D014408:Biomarkers, Tumor / Q000096:biosynthesis*; D005753:Gastric Mucosa / Q000201:enzymology / Q000378:metabolism*; D005759:Gastroenteritis / Q000175:diagnosis / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D049488:Phosphatidylethanolamine Binding Protein / Q000032:analysis / Q000096:biosynthesis*; D013274:Stomach Neoplasms / Q000175:diagnosis / Q000201:enzymology / Q000378:metabolism*
chemical_list: D014408:Biomarkers, Tumor; D049488:Phosphatidylethanolamine Binding Protein