title: Phase II studies of gemcitabine and cisplatin in heavily and minimally pretreated metastatic breast cancer.
abstract: OBJECTIVE
  Cisplatin and gemcitabine have single-agent activity in metastatic breast cancer, and preclinical data support synergy of the combination. Two parallel, phase II trials were conducted to evaluate the response rate, response duration, and toxicities of the combination. Genetic polymorphisms were analyzed for correlation with outcomes.
  
  
  METHODS
  Eligible women had measurable disease and heavily or minimally pretreated metastatic breast cancer. The heavily pretreated protocol required prior anthracycline and taxane therapy; cisplatin as part of high-dose therapy was allowed. All patients received cisplatin 25 mg/m(2) on days 1 through 4 and gemcitabine 1,000 mg/m(2) on days 2 and 8 of a 21-day cycle with prophylactic granulocyte colony-stimulating factor in the heavily pretreated group. Sera from a subset of patients were evaluated by polymerase chain reaction restriction fragment length polymorphism for polymorphisms in 10 genes of interest.
  
  
  RESULTS
  Of 136 women enrolled, 74 were heavily pretreated. Both protocols accrued to their two-stage design. The response rate for both the heavily and minimally pretreated cohorts was 26%, and the median durations of response were 5.3 and 5.9 months, respectively. In a multivariate analysis, hormone receptor-negative disease was associated with a higher response rate. The most common grades 3 or 4 toxicities were thrombocytopenia (71%), neutropenia (66%), and anemia (38%). In a subset of 55 patients, the xeroderma pigmentosum group D (XPD)-751, x-ray cross-complementing group 3 (XRCC3) and cytidine deaminase polymorphisms were significantly associated with clinical outcomes.
  
  
  CONCLUSIONS
  Combination cisplatin and gemcitabine is active in metastatic breast cancer regardless of prior therapy. Genetic polymorphisms may tailor which patients benefit from this regimen.
authors.affiliation: Department of Internal Medicine, Division of Hematology/Oncology, University of California Davis, Sacramento, CA 95817, USA. helen.chew@ucdmc.ucdavis.edu
authors.forename: Helen K
authors.initials: HK
authors.lastname: Chew
authors.forename: James H
authors.initials: JH
authors.lastname: Doroshow
authors.forename: Paul
authors.initials: P
authors.lastname: Frankel
authors.forename: Kim A
authors.initials: KA
authors.lastname: Margolin
authors.forename: George
authors.initials: G
authors.lastname: Somlo
authors.forename: Heinz-Josef
authors.initials: HJ
authors.lastname: Lenz
authors.forename: Michael
authors.initials: M
authors.lastname: Gordon
authors.forename: Wu
authors.initials: W
authors.lastname: Zhang
authors.forename: Dongyun
authors.initials: D
authors.lastname: Yang
authors.forename: Christy
authors.initials: C
authors.lastname: Russell
authors.forename: Darcy
authors.initials: D
authors.lastname: Spicer
authors.forename: Tim
authors.initials: T
authors.lastname: Synold
authors.forename: Robert
authors.initials: R
authors.lastname: Bayer
authors.forename: Alexander
authors.initials: A
authors.lastname: Hantel
authors.forename: Patrick J
authors.initials: PJ
authors.lastname: Stiff
authors.forename: Merry L
authors.initials: ML
authors.lastname: Tetef
authors.forename: David R
authors.initials: DR
authors.lastname: Gandara
authors.forename: Kathy S
authors.initials: KS
authors.lastname: Albain
mesh_terms: D000328:Adult; D000368:Aged; D000971:Antineoplastic Combined Chemotherapy Protocols / Q000627:therapeutic use*; D001943:Breast Neoplasms / Q000188:drug therapy* / Q000235:genetics / Q000473:pathology; D002945:Cisplatin / Q000008:administration & dosage / Q000009:adverse effects; D003841:Deoxycytidine / Q000008:administration & dosage / Q000009:adverse effects / Q000031:analogs & derivatives; D005260:Female; D005838:Genotype; D006801:Humans; D008875:Middle Aged; D009362:Neoplasm Metastasis; D011110:Polymorphism, Genetic; D000093542:Gemcitabine
chemical_list: D003841:Deoxycytidine; D002945:Cisplatin; D000093542:Gemcitabine