Regulation of mprF in daptomycin-nonsusceptible Staphylococcus aureus strains.
We used a well-characterized isogenic set of clinical bloodstream Staphylococcus aureus strains to study (i) regulation of mprF-mediated phosphatidylglycerol lysinylation in the contexts of in vitro daptomycin (DAP) nonsuceptibility and (ii) the role of mprF mutation in endovascular virulence. We observed a correlation between increased expression of a mutant mprF gene and reduced in vitro DAP susceptibility. There were no detectable fitness differences between strains in experimental infective endocarditis.
Yang
Soo-Jin
SJ
LA Biomedical Research Institute at Harbor-UCLA, 1124 West Carson Street, RB-2, Rm 230, Torrance, CA 90502, USA. sjyang@labiomed.org
Xiong
Yan Q
YQ
Dunman
Paul M
PM
Schrenzel
Jacques
J
François
Patrice
P
Peschel
Andreas
A
Bayer
Arnold S
AS
D019881:Aminoacyltransferases / Q000235:genetics* / Q000502:physiology; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000235:genetics* / Q000502:physiology; D017576:Daptomycin / Q000494:pharmacology*; D024881:Drug Resistance, Bacterial; D006801:Humans; D008826:Microbial Sensitivity Tests; D009154:Mutation; D013211:Staphylococcus aureus / Q000187:drug effects* / Q000235:genetics
D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D019881:Aminoacyltransferases; C429146:mprF protein, Staphylococcus aureus; D017576:Daptomycin