title: Mass Culturing of Ditylenchus dipsaci to Yield Large Quantities of Inoculum.
abstract: Methods are described for rearing large quantities of Ditylenchus dipsaci on alfalfa tissues. Nematodes and alfalfa seed were disinfected and nematodes were reared in quantities sufficient to provide a continuous supply of inoculum for our alfalfa-breeding program. Nematodes reproduced best in darkness at 20-25 C. Cultures reached maximum numbers in 3-6 wk.
authors.forename: L R
authors.initials: LR
authors.lastname: Faulkner
authors.forename: D B
authors.initials: DB
authors.lastname: Bower
authors.forename: D W
authors.initials: DW
authors.lastname: Evans
authors.forename: J H
authors.initials: JH
authors.lastname: Elgin