Simultaneous transmission mode collision-induced dissociation and ion/ion reactions for top-down protein identification/characterization using a quadrupole/time-of-flight tandem mass spectrometer.
Simultaneous transmission mode collision-induced dissociation (CID) and ion/ion proton transfer reactions have been implemented on a quadrupole/time-of-flight (TOF) tandem mass spectrometer. Reagent anions were trapped in a pressurized quadrupole collision cell by applying appropriate dc voltages while multiply protonated protein precursor ions were injected into the collision cell at energies sufficient to give rise to CID. Intact precursor ions as well as fragment ions underwent ion/ion proton transfer reactions during their passage through the collision cell and on to an orthogonal acceleration TOF mass analyzer. The resulting product ion spectrum was then submitted to deconvolution to yield a "zero-charge" spectrum, which was then matched against in silico produced spectra derived from a protein database. Dramatic improvements in the scores associated with correct matches were obtained relative to CID data without the benefit of ion/ion reactions for proteins as large as carbonic anhydrase (29 kDa). The parameters that most affect the extent of ion/ion proton transfer during transmission through the instrument include the number of anions stored in the collision cell, the amplitude of the radio frequency trapping voltage, the voltage of the LINAC potential associated with the collision cell, and the collision gas pressure. This work demonstrates that it is possible to effect whole protein tandem mass spectrometry with simultaneous CID, ion/ion reactions, and mass analysis for high duty cycle top-down protein characterization.
Liu
Jian
J
Department of Chemistry, Purdue University, West Lafayette, Indiana 47907-2084, USA.
Huang
Teng-Yi
TY
McLuckey
Scott A
SA
D000595:Amino Acid Sequence; D000818:Animals; D002256:Carbonic Anhydrases / Q000737:chemistry; D002417:Cattle; D007477:Ions / Q000737:chemistry*; D008969:Molecular Sequence Data; D009211:Myoglobin / Q000737:chemistry; D011506:Proteins / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000295:instrumentation / Q000379:methods*; D053719:Tandem Mass Spectrometry; D025801:Ubiquitin / Q000737:chemistry
D007477:Ions; D009211:Myoglobin; D011506:Proteins; D025801:Ubiquitin; D002256:Carbonic Anhydrases