title: Inflammatory and oxidative parameters in cord blood as diagnostic of early-onset neonatal sepsis: a case-control study.
abstract: OBJECTIVE
  To determine whether levels of interleukin (IL)-6, IL-10, and oxidative parameters in umbilical cord blood could contribute as an indicator of neonatal sepsis in recognized high-risk neonates.
  
  
  METHODS
  Prospective, case-control study.
  
  
  METHODS
  Neonatal intensive care unit.
  
  
  METHODS
  One hundred twenty consecutive preterm neonates who had at least one other risk factor for early-onset neonatal sepsis.
  
  
  METHODS
  None.
  
  
  RESULTS
  Umbilical cord blood samples were obtained for the determination of IL-6, IL-10, thiobarbituric acid reactive substances (TBARS), and protein carbonyls levels. Neonates were divided prospectively in two groups: control and septic. All parameters were higher in septic patients compared with control (IL-6 184.6 +/- 72.7 vs. 58.9 +/- 19.1, p < 0.01; IL-10 171.4 +/- 59.2 vs. 79.9 +/- 17.9, p < 0.01; TBARS 10.1 +/- 2.8 vs. 4.2 +/- 2.5, p < 0.01; protein carbonyls 2.4 +/- 1.2 vs. 1.15 +/- 0.5, p < 0.01, respectively, septic vs. control). In addition, these parameters were higher in the subgroup of culture-positive septic patients compared with control. IL-6 and TBARS had equivalent areas under the receiver operator characteristic (ROC) curve (0.88); IL-10 (0.80) and protein carbonyls (0.73) had lower areas. Multivariate logistic regression comparing IL-6 and TBARS in terms of the relative risk for neonatal sepsis demonstrated that TBARS was a better predictor, being independently associated with neonatal sepsis.
  
  
  CONCLUSIONS
  Our findings demonstrated that cord blood IL-6, IL-10, and oxidative stress markers were significantly higher in infants with neonatal sepsis, and only TBARS levels were independently related to the development of neonatal sepsis in our sample.
authors.affiliation: Laboratório de Fisiopatologia Experimental, Programa de Pós-Graduação em Ciências da Saúde, Unidade Acadêmica de Ciências da Saúde, Universidade do Extremo Sul Catarinense, Criciuma, SC, Brazil.
authors.forename: Ana Carolina
authors.initials: AC
authors.lastname: Cancelier
authors.forename: Fabricia
authors.initials: F
authors.lastname: Petronilho
authors.forename: Adalisa
authors.initials: A
authors.lastname: Reinke
authors.forename: Larissa
authors.initials: L
authors.lastname: Constantino
authors.forename: Roberta
authors.initials: R
authors.lastname: Machado
authors.forename: Cristiane
authors.initials: C
authors.lastname: Ritter
authors.forename: Felipe
authors.initials: F
authors.lastname: Dal-Pizzol
mesh_terms: D015415:Biomarkers / Q000097:blood; D016022:Case-Control Studies; D005260:Female; D005312:Fetal Blood / Q000737:chemistry*; D006801:Humans; D007231:Infant, Newborn; D007234:Infant, Premature; D007249:Inflammation / Q000097:blood; D007363:Intensive Care Units, Neonatal; D016753:Interleukin-10 / Q000097:blood*; D015850:Interleukin-6 / Q000097:blood*; D008297:Male; D018384:Oxidative Stress; D011446:Prospective Studies; D050050:Protein Carbonylation; D012372:ROC Curve; D012307:Risk Factors; D018805:Sepsis / Q000097:blood* / Q000175:diagnosis* / Q000382:microbiology; D013292:Streptococcus agalactiae / Q000302:isolation & purification; D017392:Thiobarbituric Acid Reactive Substances / Q000032:analysis
chemical_list: D015415:Biomarkers; D015850:Interleukin-6; D017392:Thiobarbituric Acid Reactive Substances; D016753:Interleukin-10