High Definition Oscillometry: a novel technique for non-invasive blood pressure monitoring in the cynomolgus monkey (Macaca fascicularis).
BACKGROUND
Current approaches for accurate blood pressure determination rely predominantly on invasive techniques. High Definition Oscillometry (HDO) was evaluated as a potential non-invasive approach for accurate blood pressure recordings in cynomolgus monkeys.


METHODS
In conscious animals, systolic, diastolic, mean arterial blood pressure (MAP) and pulse/minute were determined 15 times within approx. 9 minutes per individual. This session was performed during 3 consecutive days. Anaesthesia induced hypotension was controlled simultaneously with HDO and telemetry as reference.


RESULTS
Repeated measurements were highly reproducible. After procedural habituation, mean MAP was 96.2 +/- 13.7 mmHg in males and 86.9 +/- 4.3 mmHg in females. Mean intraindividual coefficients of variation ranged between 10.8% and 2.4% depending on the session and parameter. Values determined by HDO corresponded to those reported for invasive techniques.


CONCLUSIONS
Our results demonstrate, using telemetry as reference, the accuracy of HDO-based non-invasive blood pressure measurements in macaques to detect drug-related cardiovascular changes.
Schmelting
B
B
Covance Laboratories GmbH, Muenster, Germany. barthel.schmelting@covance.com
Niehoff
M
M
Egner
B
B
Korte
S H
SH
Weinbauer
G F
GF
D000778:Anesthetics, Dissociative; D000818:Animals; D001794:Blood Pressure / Q000187:drug effects; D001795:Blood Pressure Determination; D005260:Female; D007649:Ketamine; D008252:Macaca fascicularis; D008297:Male; D009991:Oscillometry / Q000379:methods*; D013686:Telemetry / Q000379:methods*
D000778:Anesthetics, Dissociative; D007649:Ketamine