title: Glucose-sensitive microcapsules from glutaraldehyde cross-linked hemoglobin and glucose oxidase.
abstract: We have fabricated the glucose-sensitive protein hollow microcapsules from hemoglobin (Hb) and glucose oxidase (GOx) via layer-by-layer technique with glutaraldehyde (GA) as a cross-linking agent. We demonstrated the coupled enzymatic reactions between Hb and GOx within capsules by means of CLSM. The real-time monitoring of the consumption of glucose may be useful for the design of a glucose sensor. Furthermore, to further study the effect of glucose on the capsules, we investigated the wall permeability of (Hb/GOx)(5) capsules. It was found that the glucose consumption could enhance the permeability of (Hb/GOx)(5) capsules. This is significant for the development of advanced drug delivery devices. Therefore, the fabricated Hb/GOx capsules may be a potential candidate for the creation of multifunctional devices.
authors.affiliation: Beijing National Laboratory for Molecular Science, International Joint Laboratory, CAS Key Laboratory of Colloid and Interface Science, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Qi
authors.forename: Xuehai
authors.initials: X
authors.lastname: Yan
authors.forename: Li
authors.initials: L
authors.lastname: Duan
authors.forename: Yue
authors.initials: Y
authors.lastname: Cui
authors.forename: Yang
authors.initials: Y
authors.lastname: Yang
authors.forename: Junbai
authors.initials: J
authors.lastname: Li