title: Circular dichroism spectroscopy has intrinsic limitations for protein secondary structure analysis.
abstract: Secondary structure content (SSC) cannot be calculated accurately from circular dichroism (CD) spectra for the majority of proteins whose three-dimensional structures have been solved. "Reliable" SSC that is significantly different from random SSC can be calculated from CD spectra only for all-alpha proteins and all-beta proteins with canonical beta-strand geometry.
authors.affiliation: Department of Biochemistry, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY 10461, USA. khraps@medusa.bioc.aecom.yu.edu
authors.forename: Sergei
authors.initials: S
authors.lastname: Khrapunov