title: Stimulation of guanylyl cyclase-D by bicarbonate.
abstract: Guanylyl cyclases (GCs) catalyze the conversion of GTP to the second messenger cGMP. While some transmembrane GCs are receptors for extracellular ligands, other transmembrane GCs such as retinal-specific GC-E and GC-F are stimulated by cellular proteins. GC-D is expressed in a special group of olfactory sensory neurons. However, the direct regulatory mechanism of GC-D activity is not completely understood. Here we have demonstrated that bicarbonate directly increases the activity of purified GC-D. Bicarbonate also increases the cGMP levels in cells expressing GC-D. These results identify bicarbonate as a small molecule that regulates GC-D.
authors.affiliation: Department of Physiology, Cornell University Weill Medical College, New York, New York 10065, USA.
authors.forename: Dagang
authors.initials: D
authors.lastname: Guo
authors.forename: J Jillian
authors.initials: JJ
authors.lastname: Zhang
authors.forename: Xin-Yun
authors.initials: XY
authors.lastname: Huang