title: Longitudinal profiling of articular cartilage degradation in osteoarthritis by high-resolution magic angle spinning 1H NMR spectroscopy: experimental study in the meniscectomized guinea pig model.
abstract: This study assessed the 1H HRMAS NMR spectroscopic profile of articular cartilage in both physiological and osteoarthitic situations. One-dimensional and two-dimensional 1H HRMAS NMR spectra were obtained from the tibial plateau cartilage of healthy and operated (unilateral medial meniscectomy and sham surgery) guinea pigs at different stages of disease, over a 6-month period. The major osteoarthritis-induced 1H HRMAS NMR changes were an increase of the N-acetyl peak of proteoglycans (at day 20 after meniscectomy) and a decrease after day 60 as the pathology evolved. These proteoglycan changes revealed by 1H HRMAS NMR analysis were validated by proteoglycan biochemistry assays. 1H HRMAS NMR analysis also evidenced a sharp increase in methylene resonances of chondrocyte membrane lipids from day 90 as a marker of apoptosis. There was an increase of the mobile methyl group of collagen at day 120, which was associated with collagen breakdown. 1H HRMAS NMR analysis provided a multifactorial and sequential picture of cartilage degradation at the extracellular matrix and chondrocyte levels.
authors.affiliation: EA 4231, University d'Auvergne, INSERM UMR 484, Clermont-Ferrand, F-63005 France. michele.borel@inserm.fr
authors.forename: Michele
authors.initials: M
authors.lastname: Borel
authors.forename: Philippe
authors.initials: P
authors.lastname: Pastoureau
authors.forename: Janine
authors.initials: J
authors.lastname: Papon
authors.forename: Jean Claude
authors.initials: JC
authors.lastname: Madelmont
authors.forename: Nicole
authors.initials: N
authors.lastname: Moins
authors.forename: Jean
authors.initials: J
authors.lastname: Maublant
authors.forename: Elisabeth
authors.initials: E
authors.lastname: Miot-Noirault