title: Using PATIMDB to create bacterial transposon insertion mutant libraries.
abstract: PATIMDB is a software package for facilitating the generation of transposon mutant insertion libraries. The software has two main functions: process tracking and automated sequence analysis. The process tracking function specifically includes recording the status and fates of multiwell plates and samples in various stages of library construction. Automated sequence analysis refers specifically to the pipeline of sequence analysis starting with ABI files from a sequencing facility and ending with insertion location identifications. The protocols in this unit describe installation and use of PATIMDB software.
authors.affiliation: Massachusetts General Hospital, Boston, Massachusetts, USA.
authors.forename: Jonathan M
authors.initials: JM
authors.lastname: Urbach
authors.forename: Tao
authors.initials: T
authors.lastname: Wei
authors.forename: Nicole
authors.initials: N
authors.lastname: Liberati
authors.forename: Daniel
authors.initials: D
authors.lastname: Grenfell-Lee
authors.forename: Jacinto
authors.initials: J
authors.lastname: Villanueva
authors.forename: Gang
authors.initials: G
authors.lastname: Wu
authors.forename: Frederick M
authors.initials: FM
authors.lastname: Ausubel