Detection of hydrogen peroxide by lactoperoxidase-mediated dityrosine formation.
The aim of this work was to study the dityrosine-forming activity of lactoperoxidase (LPO) and its potential application for measuring hydrogen peroxide (H2O2). It was observed that LPO was able to form dityrosine at low H2O2 concentrations. Since dityrosine concentration could be measured in a simple fluorimetric reaction, this activity of the enzyme was utilized for the measurement of H2O2 production in different systems. These experiments successfully measured the activity of NADPH oxidase 4 (Nox4) by this method. It was concluded that LPO-mediated dityrosine formation offers a simple way for H2O2 measurement.
Donkó
Agnes
A
Department of Physiology, Semmelweis University, Faculty of Medicine, Budapest, Hungary.
Orient
Anna
A
Szabó
Pál T
PT
Németh
Gábor
G
Vántus
Tibor
T
Kéri
György
G
Orfi
László
L
Hunyady
László
L
Buday
László
L
Geiszt
Miklós
M