title: SATB1 regulates beta-like globin genes through matrix related nuclear relocation of the cluster.
abstract: The nuclear location and relocation of genes play crucial regulatory roles in gene expression. SATB1, a MAR-binding protein, has been found to regulate beta-like globin genes through chromatin remodeling. In this study, we generated K562 cells over-expressing wild-type or nuclear matrix targeting sequences (NMTS)-deficient SATB1 and found that like wild-type SATB1, NMTS-deficient SATB1 induces out loop of beta-globin cluster from its chromosome territory (CT), while it is unable to associate the cluster with the nuclear matrix as wild-type SATB1 does and had no regulatory functions to the beta-globin cluster. Besides, our data showed that the transacting factor occupancies and chromatin modifications at beta-globin cluster were differentially affected by wild-type and NMTS-deficient SATB1. These results indicate that SATB1 regulates beta-like globin genes at the nuclear level interlaced with chromatin and DNA level, and emphasize the nuclear matrix binding activity of SATB1 to its regulatory function.
authors.affiliation: Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, Peking Union Medical College, Dong Dan San Tiao, Beijing, PR China.
authors.forename: Huan
authors.initials: H
authors.lastname: Gong
authors.forename: Zhao
authors.initials: Z
authors.lastname: Wang
authors.forename: Guo-wei
authors.initials: GW
authors.lastname: Zhao
authors.forename: Xiang
authors.initials: X
authors.lastname: Lv
authors.forename: Gong-hong
authors.initials: GH
authors.lastname: Wei
authors.forename: Li
authors.initials: L
authors.lastname: Wang
authors.forename: De-pei
authors.initials: DP
authors.lastname: Liu
authors.forename: Chih-chuan
authors.initials: CC
authors.lastname: Liang