Solid phase subtractive cloning in differentially expressed genes identification.
We developed an array-based subtractive hybridization system for one-step high-throughput subtraction. We printed subtractor RNA up to 10.000 times obtaining an excellent contact surface using a little amount of RNA. During hybridization cDNA, common to subtractor and target samples, remains attached to slide immobilized RNA, leaving free in solution target specific cDNA which after retrieval is cloned.
D'Urso
Oscar F
OF
Promeridio Association, Non-Profit Research Association, San Michele Salentino, BR, Italy. doscarferna@gmail.com
D'Urso
Pietro I
PI
Storelli
Carlo
C
Marsigliante
Santo
S