title: A streamlined method for rapid and sensitive chromatin immunoprecipitation.
abstract: We report a streamlined procedure to efficiently carry samples from chromatin to qPCR-compatible DNA in as little as 4 h. We use this streamlined ChIP to quantify histone H3 modifications at active (cad) and repressed (T early alpha) promoters in a Rag1-deficient pro-T cell line after 1-2 h IP. We further show that the protocol readily quantified histone modifications in chromatin from 10(4) Rag-deficient DN thymocytes. Taken together, these data outline a simple, cost-effective procedure for efficient ChIP analysis.
authors.affiliation: Department of Microbiology, North Carolina State University, Raleigh, NC 27695, USA. mlsikes@ncsu.edu
authors.forename: Michael L
authors.initials: ML
authors.lastname: Sikes
authors.forename: Justin M
authors.initials: JM
authors.lastname: Bradshaw
authors.forename: Wendell T
authors.initials: WT
authors.lastname: Ivory
authors.forename: Jessica L
authors.initials: JL
authors.lastname: Lunsford
authors.forename: Ruth E
authors.initials: RE
authors.lastname: McMillan
authors.forename: Clayton R
authors.initials: CR
authors.lastname: Morrison