title: A sensitive and selective fluorescent thiol probe in water based on the conjugate 1,4-addition of thiols to alpha,beta-unsaturated ketones.
abstract: Compound 1 was designed and synthesized as a new fluorescent thiol probe. Probe 1 was constructed on the basis of the conjugate 1,4-addition of thiols to alpha,beta-unsaturated ketones. Notably, probe 1 has suitable water solubility, which allows the sensing assay to be performed in water. Probe 1 is highly sensitive for thiols with a 211-fold fluorescence dynamic range and a low detection limit of 9.25x10(-7) M. The major features of probe 1 also include a high selectivity for thiols over other relevant biological species, excitation and emission in the visible region, rapid functioning at pH 7.4, and a good linear relationship between the fluorescence signal and the thiol concentration. Accordingly, these desirable characteristics may render probe 1 as potentially useful for biological applications.
authors.affiliation: State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, Hunan 410082, PR China. Weiyinglin@hnu.cn
authors.forename: Weiying
authors.initials: W
authors.lastname: Lin
authors.forename: Lin
authors.initials: L
authors.lastname: Yuan
authors.forename: Zengmei
authors.initials: Z
authors.lastname: Cao
authors.forename: Yanming
authors.initials: Y
authors.lastname: Feng
authors.forename: Lingliang
authors.initials: L
authors.lastname: Long