Patterned growth and differentiation of human cord blood-derived neural stem cells on bio-functionalized surfaces.
Bio-functionalized surfaces were prepared to study the adherence and differentiation capacity of neural stem cells derived from human umbilical cord blood (HUCB-NSC). Cell growth platforms containing arranged arrays of adhesive molecules were created by microcontact printing on a biologically inert surface. Biomolecules used to prepare microarray platforms included the extracellular matrix protein fibronectin and the polyaminoacid poly-L-lysine. HUCB-NSC plated on microplatforms at various serum conditions showed serum and molecule type dependent capacity for adhesion and differentiation. Poly-L-lysine allowed the maintenance of stem-like non differentiated cells attached to the surface, whereas fibronectin promoted spreading and neural commitment. Serum deprivation did not influence the attachment of HUCB-NSC to fibronectin, but significantly enhanced the attachment to poly-L-lysine and promoted dBcAMP induced neuronal differentiation. A bio-pattern of squares with interconnecting lines was used to guide neuronal differentiation by directing cell protrusion outgrowth. Tailoring the geometry of the bio-pattern enabled directing and monitoring of the neural stem cells. development in the large scale multiparameter biotests.
Buzanska
Leonora
L
In Vitro Toxicology/ECVAM, Institute for Health and Consumer Protection, European Commission, Joint Research Centre, Ispra, Italy. buzanska@cmdik.pan.pl.
Ruiz
Ana
A
Zychowicz
Marzena
M
Rauscher
Hubert
H
Ceriotti
Laura
L
Rossi
Francois
F
Colpo
Pascal
P
Domańska-Janik
Krystyna
K
Coecke
Sandra
S