title: Ethical and technical considerations for the creation of cell lines in the head & neck and tissue harvesting for research and drug development (Part I): techniques of tissue harvesting and propagation.
abstract: BACKGROUND
  Although much has been published for the development of cell lines, these were lab based and developed for scientific technical staff.
  
  
  OBJECTIVE
  We present a simple and successful protocol for the development of cell lines and tissue harvesting for the clinical scientist. We also discuss the ethical implications of tissue retention and present a generic consent form.
  
  
  CONCLUSIONS
  The advantages of hospital-based cell line creation are numerous. We can be more certain that cell lines are developed from the particular tissues of interest and accurate anatomical and appropriate clinico-pathological control tissues are also harvested. We can also be certain of less cell line cross contamination.
authors.affiliation: Head & Neck Centre, University College London Hospital, London, UK. mrtupile@yahoo.com
authors.forename: Tahwinder
authors.initials: T
authors.lastname: Upile
authors.forename: Waseem
authors.initials: W
authors.lastname: Jerjes
authors.forename: Panagiotis
authors.initials: P
authors.lastname: Kafas
authors.forename: Sandeep U
authors.initials: SU
authors.lastname: Singh
authors.forename: Holger
authors.initials: H
authors.lastname: Sudhoff
authors.forename: Jaspal
authors.initials: J
authors.lastname: Mahil
authors.forename: Ann
authors.initials: A
authors.lastname: Sandison
authors.forename: Colin
authors.initials: C
authors.lastname: Hopper