Ethical and technical considerations for the creation of cell lines in the head & neck and tissue harvesting for research and drug development (Part I): techniques of tissue harvesting and propagation.
BACKGROUND
Although much has been published for the development of cell lines, these were lab based and developed for scientific technical staff.


OBJECTIVE
We present a simple and successful protocol for the development of cell lines and tissue harvesting for the clinical scientist. We also discuss the ethical implications of tissue retention and present a generic consent form.


CONCLUSIONS
The advantages of hospital-based cell line creation are numerous. We can be more certain that cell lines are developed from the particular tissues of interest and accurate anatomical and appropriate clinico-pathological control tissues are also harvested. We can also be certain of less cell line cross contamination.
Upile
Tahwinder
T
Head & Neck Centre, University College London Hospital, London, UK. mrtupile@yahoo.com
Jerjes
Waseem
W
Kafas
Panagiotis
P
Singh
Sandeep U
SU
Sudhoff
Holger
H
Mahil
Jaspal
J
Sandison
Ann
A
Hopper
Colin
C