Differential expression of H19 and vitamin D3 upregulated protein 1 as a mechanism of the modulatory effects of high virgin olive oil and high corn oil diets on experimental mammary tumours.
Dietary lipids have a role in the aetiology of breast cancer. We have reported earlier that a high corn oil diet downregulates H19 and vitamin D3 upregulated protein 1 (VDUP1) messenger RNA (mRNA) expression in rat dimethylbenz (alpha) anthracene-induced mammary adenocarcinomas in comparison with the control low-fat diet, this effect being associated with a higher degree of tumour malignancy. This result was compatible with the stimulating effect of this diet. In this study we have investigated the influence of a high extra virgin olive diet on H19 and VDUP1 mRNA and/or protein expression. We have shown earlier that this high-fat diet confers to the tumours a more benign phenotype in accordance with its potential protective effect on mammary cancer. We have also analysed the effects on the mRNA and protein expression of insulin-like growth factor-2 , in close relation with H19, and the expression and activity of the thioredoxin protein, negatively regulated by VDUP1. mRNA and protein expression were analysed by chemiluminescent northern blot and western blot, respectively. Thioredoxin activity was determined by the insulin-reducing assay. The results showed that the high olive oil diet does not change the tumour expression of H19 and VDUP1. Moreover, tumours from the animals fed this diet displayed higher levels of the insulin-like growth factor-2 mRNAs, which are related to a higher rate of degradation or a lower traducibility. Finally, tumour expression and activity levels of thioredoxin-1 protein did not change irrespective of the diet. These results suggest that the differential effects of high olive oil and high corn oil diets on mammary cancer are exerted by means of a different, specific influence on gene expression.
Solanas
Montserrat
M
Department of Cell Biology, Physiology and Immunology, Medical Physiology Unit, Medicine School, Universitat Autònoma de Barcelona, Bellaterra, Barcelona, Spain.
Moral
Raquel
R
Garcia
Gemma
G
Grau
Laura
L
Vela
Elena
E
Escrich
Raquel
R
Costa
Irmgard
I
Escrich
Eduard
E