title: Diagnosis of acute malaria by laser based cell counter with comparison of conventional and recent techniques in Indian scenario.
abstract: Malaria is one of the most important parasitic diseases in humans affecting 103 countries worldwide.
  
  
  OBJECTIVE
  The present study aims to determine the diagnostic utility of cell counter data--hemoglobin, total leukocyte count, platelet count and depolarized laser light (DLL)-based purple-coded events (PCEs) in detection of acute malaria. This is a retrospective study of 523 patient data that came for complete blood count for the first time.
  
  
  RESULTS
  One hundred thirty-five of the 523 patients showed microscopic evidence of malaria. Platelet count showed the highest sensitivity of 77.77% (105/135). PCEs (> or = 1) showed 43.7% (59/135) sensitivity.
  
  
  CONCLUSIONS
  It is concluded that a low platelet count (< 150 x 109/L) is a good hematological parameter for presumptive diagnosis of malaria. If we change the cut-off for PCEs from > or = 1 to > or = 2, the sensitivity would be 56.29% (76/135) and the specificity would be 94.58% (367/388), respectively. The sensitivity of DLL was low, particularly with a low parasitic index (PI). The number of PCEs does not correlate with the PI. The cut-off number of PCEs in DLL-based malaria detection should be modified in highly endemic areas.
authors.affiliation: Department of Pathology, Smt NHL Municipal Medical College, Ellisbridge, Ahmedabad-380 006, Gujarat, India. rathod_pathology@rediffmail.com
authors.forename: Dinesh A
authors.initials: DA
authors.lastname: Rathod
authors.forename: Viral
authors.initials: V
authors.lastname: Patel
authors.forename: Amarjeet A
authors.initials: AA
authors.lastname: Kaur
authors.forename: Vinod D
authors.initials: VD
authors.lastname: Patel
authors.forename: Devangi D
authors.initials: DD
authors.lastname: Patel