title: Genetic analysis of coronary artery disease single-nucleotide polymorphisms in diabetic nephropathy.
abstract: BACKGROUND
  Diabetic nephropathy is a leading cause of end-stage renal disease. Premature mortality is common in patients with nephropathy, largely due to cardiovascular disease. Genetic variants implicated in macrovascular disease are therefore excellent candidates to assess for association with diabetic nephropathy. Recent genome-wide association studies have identified a total of 15 single-nucleotide polymorphisms (SNPs) that are reproducibly associated with cardiovascular disease.
  
  
  METHODS
  We initially assessed these SNPs for association in UK type 1 diabetic patients with (cases; n = 597) and without (controls; n = 502) nephropathy using iPLEX(TM) and TaqMan(R) assays. Replication studies were performed with DNA genotyped in a total of 2668 individuals from the British Isles.
  
  
  RESULTS
  One SNP (rs4420638) on chromosome 19q13 was found to be significantly associated with diabetic nephropathy before (P = 0.0002) and after correction for multiple testing (P(corrected) = 0.002). We replicated this finding in a phenotypically similar case-control collection comprising 709 individuals with type 1 diabetes (P = 0.002; combined P < 0.00001; OR = 1.54, 95% CI: 1.29-1.84).
  
  
  CONCLUSIONS
  Our case-control data suggest that rs4420638, or a functional SNP in linkage disequilibrium with this SNP, may be associated with diabetic nephropathy.
authors.affiliation: Nephrology Research Group, Queen's University of Belfast, Belfast, Northern Ireland. a.j.mcknight@qub.ac.uk
authors.forename: Amy Jayne
authors.initials: AJ
authors.lastname: McKnight
authors.forename: Alexander P
authors.initials: AP
authors.lastname: Maxwell
authors.forename: Damian G
authors.initials: DG
authors.lastname: Fogarty
authors.forename: Denise
authors.initials: D
authors.lastname: Sadlier
authors.forename: David A
authors.initials: DA
authors.lastname: Savage