Oxidative enzymatic alkene cleavage: indications for a nonclassical enzyme mechanism.
An enzyme preparation of Trametes hirsuta cleaves alkenes following neither the classical dioxygenase mechanism nor via a monooxygenase mechanism. A catalytic cycle for an alternative enzymatic alkene cleavage was proposed, whereby two oxygen atoms derived from two different oxygen molecules are incorporated into the product(s).
Lara
Miguel
M
Department of Chemistry, Organic and Bioorganic Chemistry, University of Graz, Heinrichstrasse 28, 8010 Graz, Austria.
Mutti
Francesco G
FG
Glueck
Silvia M
SM
Kroutil
Wolfgang
W