Expanding the scope of protein trans-splicing to fragment ligation of an integral membrane protein: towards modulation of porin-based ion channels by chemical modification.
It's raining, it's porin: Fragment ligation of OmpF ion channels was achieved by using the split Psp-GBD Pol intein; this allowed reconstitution of active trimeric porin. In combination with cysteine modification at an internal position, the porin's conductance properties were altered.
Brenzel
Steffen
S
Technische Universität Dortmund, Fakultät Chemie-Chemische Biologie, Otto-Hahn-Strasse, Dortmund, Germany.
Cebi
Menekse
M
Reiss
Philipp
P
Koert
Ulrich
U
Mootz
Henning D
HD