title: Expressed sequence tags from the zhikong scallop (Chlamys farreri): discovery and annotation of host-defense genes.
abstract: A high-quality cDNA library was constructed from whole body tissues of the zhikong scallop, Chlamys farreri, challenged by Listonella anguillarum. A total of 5720 clones were sequenced, yielding 5123 expressed sequence tags (ESTs). Among the 3326 unique genes identified, 2289 (69%) genes had no significant (E-value < 1e-5) matches to known sequences in public databases and 194 (6%) matched proteins of unknown functions. The remaining 843 (25%) genes that exhibited homology with genes of known functions, showed broad involvement in metabolic processes (31%), cell structure and motility (20%), gene and protein expression (12%), cell signaling and cell communication (8%), cell division (4%), and notably, 25% of those genes were related to immune function. They included stress response genes, complement-like genes, proteinase and proteinase inhibitors, immune recognition receptors and immune effectors. The EST collection obtained in this study provides a useful resource for gene discovery and especially for the identification of host-defense genes and systems in scallops and other molluscs.
authors.affiliation: The Key laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, Shandong, PR China.
authors.forename: Lingling
authors.initials: L
authors.lastname: Wang
authors.forename: Linsheng
authors.initials: L
authors.lastname: Song
authors.forename: Jianmin
authors.initials: J
authors.lastname: Zhao
authors.forename: Limei
authors.initials: L
authors.lastname: Qiu
authors.forename: Huan
authors.initials: H
authors.lastname: Zhang
authors.forename: Wei
authors.initials: W
authors.lastname: Xu
authors.forename: Honglei
authors.initials: H
authors.lastname: Li
authors.forename: Chenhua
authors.initials: C
authors.lastname: Li
authors.forename: Longtao
authors.initials: L
authors.lastname: Wu
authors.forename: Ximing
authors.initials: X
authors.lastname: Guo