title: Overexpression, purification and crystallization of a thermostable DNA ligase from the archaeon Thermococcus sp. 1519.
abstract: DNA ligases catalyze the sealing of 5'-phosphate and 3'-hydroxyl termini at single-strand breaks in double-stranded DNA and their function is essential to maintain the integrity of the genome in DNA metabolism. An ATP-dependent DNA ligase from the archaeon Thermococcus sp. 1519 was overexpressed, purified and crystallized. Crystals were obtained using the hanging-drop vapour-diffusion method employing 35%(v/v) Tacsimate pH 7.0 as a precipitant and diffracted X-rays to 3.09 A resolution. They belonged to space group P4(1)2(1)2, with unit-cell parameters a = b = 79.7, c = 182.6 A.
authors.affiliation: Bach Institute of Biochemistry RAS, Leninsky Prospect 33, 119071 Moscow, Russia. eubez@yandex.ru
authors.forename: E Y
authors.initials: EY
authors.lastname: Bezsudnova
authors.forename: M V
authors.initials: MV
authors.lastname: Kovalchuk
authors.forename: A V
authors.initials: AV
authors.lastname: Mardanov
authors.forename: K M
authors.initials: KM
authors.lastname: Poliakov
authors.forename: V O
authors.initials: VO
authors.lastname: Popov
authors.forename: N V
authors.initials: NV
authors.lastname: Ravin
authors.forename: K G
authors.initials: KG
authors.lastname: Skryabin
authors.forename: V A
authors.initials: VA
authors.lastname: Smagin
authors.forename: T N
authors.initials: TN
authors.lastname: Stekhanova
authors.forename: T V
authors.initials: TV
authors.lastname: Tikhonova