title: Detection of hydrogen peroxide by lactoperoxidase-mediated dityrosine formation.
abstract: The aim of this work was to study the dityrosine-forming activity of lactoperoxidase (LPO) and its potential application for measuring hydrogen peroxide (H2O2). It was observed that LPO was able to form dityrosine at low H2O2 concentrations. Since dityrosine concentration could be measured in a simple fluorimetric reaction, this activity of the enzyme was utilized for the measurement of H2O2 production in different systems. These experiments successfully measured the activity of NADPH oxidase 4 (Nox4) by this method. It was concluded that LPO-mediated dityrosine formation offers a simple way for H2O2 measurement.
authors.affiliation: Department of Physiology, Semmelweis University, Faculty of Medicine, Budapest, Hungary.
authors.forename: Agnes
authors.initials: A
authors.lastname: Donkó
authors.forename: Anna
authors.initials: A
authors.lastname: Orient
authors.forename: Pál T
authors.initials: PT
authors.lastname: Szabó
authors.forename: Gábor
authors.initials: G
authors.lastname: Németh
authors.forename: Tibor
authors.initials: T
authors.lastname: Vántus
authors.forename: György
authors.initials: G
authors.lastname: Kéri
authors.forename: László
authors.initials: L
authors.lastname: Orfi
authors.forename: László
authors.initials: L
authors.lastname: Hunyady
authors.forename: László
authors.initials: L
authors.lastname: Buday
authors.forename: Miklós
authors.initials: M
authors.lastname: Geiszt