High speed unsupervised fluorescence lifetime imaging confocal multiwell plate reader for high content analysis.
We report an automated optically sectioning fluorescence lifetime imaging (FLIM) multiwell plate reader for high content analysis (HCA) in drug discovery and accelerated research in cell biology. The system utilizes a Nipkow disc confocal microscope and performs unsupervised FLIM with autofocus, automatic setting of acquisition parameters and automated localisation of cells in the field of view. We demonstrate its applications to test dye solutions, fixed and live cells and FLIM-FRET.
Talbot
Clifford B
CB
Photonics Group, Imperial College London, London SW72AZ, UK. clifford.talbot@imperial.ac.uk
McGinty
James
J
Grant
David M
DM
McGhee
Ewan J
EJ
Owen
Dylan M
DM
Zhang
Wei
W
Bunney
Tom D
TD
Munro
Ian
I
Isherwood
Beverly
B
Eagle
Rob
R
Hargreaves
Andre
A
Dunsby
Chris
C
Neil
Mark A A
MA
French
Paul M W
PM