A streamlined method for rapid and sensitive chromatin immunoprecipitation.
We report a streamlined procedure to efficiently carry samples from chromatin to qPCR-compatible DNA in as little as 4 h. We use this streamlined ChIP to quantify histone H3 modifications at active (cad) and repressed (T early alpha) promoters in a Rag1-deficient pro-T cell line after 1-2 h IP. We further show that the protocol readily quantified histone modifications in chromatin from 10(4) Rag-deficient DN thymocytes. Taken together, these data outline a simple, cost-effective procedure for efficient ChIP analysis.
Sikes
Michael L
ML
Department of Microbiology, North Carolina State University, Raleigh, NC 27695, USA. mlsikes@ncsu.edu
Bradshaw
Justin M
JM
Ivory
Wendell T
WT
Lunsford
Jessica L
JL
McMillan
Ruth E
RE
Morrison
Clayton R
CR