title: Development of an immunoassay to measure progesterone using printed biosensors, and its application to the assessment of ovarian function in the numbat (Myrmecobius fasciatus).
abstract: A biosensor system was developed to measure progesterone levels in the urine of female numbats (Myrmecobius fasciata) as an index of ovarian function. Screen printed sensors were coated with a monoclonal progesterone antibody, and incubated in a mixture of sample/standard and progesterone-3-CMO-horseradish peroxidase (HRP). The difference in potential between the working and reference electrode was measured, after exposure to an HRP substrate. EIA and biosensor standard curves showed parallelism, and the biosensor gave values similar (r = 0.83) to the conventional EIA. Progesterone concentrations at different stages of the oestrus cycle were not significantly different to those obtained by EIA.
authors.affiliation: School of Veterinary and Biomedical Sciences, Murdoch University, Western Australia, Australia. w.ditcham@murdoch.edu.au
authors.forename: Will
authors.initials: W
authors.lastname: Ditcham
authors.forename: Carly
authors.initials: C
authors.lastname: Palmer
authors.forename: Kristin
authors.initials: K
authors.lastname: Warren
authors.forename: Cree
authors.initials: C
authors.lastname: Monaghan
authors.forename: Wendy
authors.initials: W
authors.lastname: Kappelle
authors.forename: Phillip
authors.initials: P
authors.lastname: Matson