Utilization of a right-handed coiled-coil protein from archaebacterium Staphylothermus marinus as a carrier for cisplatin.
BACKGROUND
The nano-sized right-handed coiled-coil (RHCC) protein, originating from the archaebacterium Staphylothermus marinus, is stable at high salt concentrations, high temperatures, high pressures and extremes of pH. Its crystal structure reveals four hydrophobic cavities which can incorporate heavy metals. Nano-sized compounds have been used to carry cytotoxic drugs to tumours, avoiding delivery to healthy tissue, in part due to enhanced permeability in tumour blood vessels (enhanced permeability and retention effect).


METHODS
The ability of RHCC to carry the platinum-containing chemotherapeutic drug cisplatin to cells, while retaining the cytotoxic potential was tested both in vitro and in vivo.


RESULTS
RHCC was able to bind and enter cells in vitro and was not severely toxic or immunogenic in mice. Moreover, RHCC incorporated cisplatin, without inhibiting the cytotoxic potential of the drug against tumour cell lines in vitro or in vivo.


CONCLUSIONS
RHCC can be used as a carrier of cisplatin without abrogating the effect of the drug.
Eriksson
Mathilda
M
Karolinska Institutet, Department of Oncology-Pathology, Cancer Center Karolinska R8:01, Karolinska University Hospital, 171 76 Stockholm.
Hassan
Saadia
S
Larsson
Rolf
R
Linder
Stig
S
Ramqvist
Torbjörn
T
Lövborg
Henrik
H
Vikinge
Trine
T
Figgemeier
Egbert
E
Müller
Jürgen
J
Stetefeld
Jörg
J
Dalianis
Tina
T
Ozbek
Suat
S