title: [Isolation of tick-borne borreliosis agent from blood of patients].
abstract: During spring-autumn period of 2006 Borrelia were isolated for the first time in Russia from blood of 79 patients treated in Perm City Clinical Hospital for Infectious Diseases No. 1 with diagnosis "tickborne borreliosis, manifestive form with migrating erythema, localized stage". Ten primary isolates (12.7% of total seeded samples) were obtained by seeding plasma samples on the BSK medium. Their subsequent identification by polymerase chain reaction-restriction fragments length polymorphism revealed presence of Borrelia garinii NT29 in all patients. Length of sequenced fragment of rrfA-rrlB region was 253 b.p. Seven isolates had 100% and 3 - 99.6% similarity with typical strain NT29 (L30130). Nucleotide sequences of 4 obtained isolates were deposited in GenBank database (No. AM932199 - AM932202). It was proposed that B. garinii NT29 more frequently than other Borrelia species can be an etiologic agent of tick-borne borreliosis not only in Perm region but also in whole Russia.
authors.forename: V V
authors.initials: VV
authors.lastname: Nefedova
authors.forename: V Iu
authors.initials: VIu
authors.lastname: Teterin
authors.forename: E I
authors.initials: EI
authors.lastname: Korenberg
authors.forename: N B
authors.initials: NB
authors.lastname: Gorelova
authors.forename: N N
authors.initials: NN
authors.lastname: Vorob'eva
authors.forename: V I
authors.initials: VI
authors.lastname: Frizen