One-step PCR for the identification of multiple antimicrobial resistance in Neisseria gonorrhoeae.
One-step multiplex PCR was developed for the identification of gonococci and antimicrobial-resistant profiles. From forty Neisseria gonorrhoeae isolates, the penicillinase-producing N. gonorrhoeae (PPNG), the high-level tetracycline-resistant N. gonorrhoeae (TRNG), and the ciprofloxacin-resistant N. gonorrhoeae (CRNG) were successfully classified. Our method provides expediency and benefit to epidemiology and antimicrobial-resistance mobility with 100% sensitivity and specificity for gonococcal-detection. The detection limit was 500 CFU/reaction.
Lawung
Ratana
R
Department of Clinical Microbiology, Faculty of Medical Technology, Mahidol University, Bangkok 10700, Thailand.
Cherdtrakulkiat
Rungrot
R
Charoenwatanachokchai
Angkana
A
Nabu
Sunanta
S
Suksaluk
Wanvisa
W
Prachayasittikul
Virapong
V