The development and evaluation of monoclonal antibodies for the detection of polycyclic aromatic hydrocarbons.
A highly sensitive enzyme-linked immunosorbent assay (ELISA) for the detection of 3- to 5-ring polycyclic aromatic hydrocarbons (PAHs) has been developed. A functional derivative of dibenzothiophene was synthesized and covalently linked to carrier proteins that were used to produce monoclonal antibodies (mAbs). During the conjugation step, the conjugation efficiency was improved by the presence of 25% N,N-dimethylformamide (DMF). Antibodies were selected based on a competitive inhibition assay to isolate those with the highest sensitivity for free PAHs. When using the mAb in an ELISA format, free PAHs were detected at a concentration as low as 0.1 microg/L (0.1 ppb) in aqueous samples.
Spier
Candace R
CR
Department of Environmental and Aquatic Animal Health, Virginia Institute of Marine Science, College of William & Mary, Gloucester Point, VA 23062, USA. cspier@vims.edu
Bromage
Erin S
ES
Harris
Thomas M
TM
Unger
Michael A
MA
Kaattari
Stephen L
SL