title: Replication-defective adenovirus vectors with multiple deletions do not induce measurable vector-specific T cells in human trials.
abstract: The magnitude and character of adenovirus serotype 5 (Ad5)-specific T cells were determined in volunteers with and without preexisting neutralizing antibodies (NAs) to Ad5 who received replication-defective Ad5 (rAd5)-based human immunodeficiency virus vaccines. There was no correlation between T-cell responses and NAs to Ad5. There was no increase in magnitude or activation state of Ad5-specific CD4(+) T cells at time points where antibodies to Ad5 and T-cell responses to the recombinant gene products could be measured. These data indicate that rAd5-based vaccines containing deletions in the E1, E3, and E4 regions do not induce appreciable expansion of vector-specific CD4(+) T cells.
authors.affiliation: Immunology Laboratory, Immunology Core Section, Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA. rkoup@mail.nih.gov
authors.forename: Richard A
authors.initials: RA
authors.lastname: Koup
authors.forename: Laurie
authors.initials: L
authors.lastname: Lamoreaux
authors.forename: David
authors.initials: D
authors.lastname: Zarkowsky
authors.forename: Robert T
authors.initials: RT
authors.lastname: Bailer
authors.forename: C Richter
authors.initials: CR
authors.lastname: King
authors.forename: Jason G D
authors.initials: JG
authors.lastname: Gall
authors.forename: Douglas E
authors.initials: DE
authors.lastname: Brough
authors.forename: Barney S
authors.initials: BS
authors.lastname: Graham
authors.forename: Mario
authors.initials: M
authors.lastname: Roederer