title: Expression, purification and preliminary X-ray crystallographic analysis of the chicken MHC class I molecule YF1*7.1.
abstract: YF1*7.1 is an allele of a polymorphic major histocompatibility complex (MHC) class I-like locus within the chicken Y gene complex. With the aim of understanding the possible role of the YF1*7.1 molecule in antigen presentation, the complex of YF1*7.1 heavy chain and beta(2)-microglobulin was reconstituted and purified without a peptide. Crystals diffracted synchrotron radiation to 1.32 A resolution and belonged to the monoclinic space group P2(1). The phase problem was solved by molecular replacement. A detailed examination of the structure may provide insight into the type of ligand that could be bound by the YF1*7.1 molecule.
authors.affiliation: Institut für Immungenetik, Charité-Universitätsmedizin Berlin, Freie Universität Berlin, Berlin, Germany.
authors.forename: Chee Seng
authors.initials: CS
authors.lastname: Hee
authors.forename: Song
authors.initials: S
authors.lastname: Gao
authors.forename: Marcia M
authors.initials: MM
authors.lastname: Miller
authors.forename: Ronald M
authors.initials: RM
authors.lastname: Goto
authors.forename: Andreas
authors.initials: A
authors.lastname: Ziegler
authors.forename: Oliver
authors.initials: O
authors.lastname: Daumke
authors.forename: Barbara
authors.initials: B
authors.lastname: Uchanska-Ziegler