title: Glycosylation engineering of spinosyn analogues containing an L-olivose moiety.
abstract: Biosynthetic genes encoding proteins involved in the first steps of deoxyhexose biosynthesis from D-glucose-1-phosphate were expressed in Saccharopolyspora erythraea. The resulting mutant was able to accumulate and utilise TDP-L-olivose. Co-expression of the spinosyn glycosyl transferase SpnP in the resulting mutant endowed upon it the ability to biotransform exogenously added spinosyn aglycones to yield novel spinosyn analogues.
authors.affiliation: Biotica Technology Ltd, Chesterford Research Park, Cambridge, UK CB10 1XL.
authors.forename: Sabine
authors.initials: S
authors.lastname: Gaisser
authors.forename: Isabelle
authors.initials: I
authors.lastname: Carletti
authors.forename: Ursula
authors.initials: U
authors.lastname: Schell
authors.forename: Paul R
authors.initials: PR
authors.lastname: Graupner
authors.forename: Thomas C
authors.initials: TC
authors.lastname: Sparks
authors.forename: Christine J
authors.initials: CJ
authors.lastname: Martin
authors.forename: Barrie
authors.initials: B
authors.lastname: Wilkinson