title: Synthesis of N-linked glycopeptides on solid support and their evaluation as protease substrates.
abstract: A range of glycopeptides containing protease cleavage sites were synthesized on solid support using Fmoc-based solid phase glycopeptide synthesis. The immobilized peptides were studied as substrates for the proteases chymotrypsin and thermolysin. For chymotrypsin, N-glycosylation of an Asn residue at the P(2) site appears to reduce hydrolysis whereas glycosylation of the P(1) site does not appear to affect peptide hydrolysis by thermolysin.
authors.affiliation: School of Chemistry, The University of Edinburgh, Kings Buildings, West Mains Road, Edinburgh EH9 3JJ, UK.
authors.forename: Mallesham
authors.initials: M
authors.lastname: Bejugam
authors.forename: Beatrice A
authors.initials: BA
authors.lastname: Maltman
authors.forename: Sabine L
authors.initials: SL
authors.lastname: Flitsch