title: Ligand-directed tosyl chemistry for protein labeling in vivo.
abstract: Here we describe a method for the site-selective attachment of synthetic molecules into specific 'endogenous' proteins in vivo using ligand-directed tosyl (LDT) chemistry. This approach was applied not only for chemically labeling proteins in living cells, tissues and mice but also for constructing a biosensor directly inside cells without genetic engineering. These data establish LDT chemistry as a new tool for the study and manipulation of biological systems.
authors.affiliation: Department of Synthetic Chemistry and Biological Chemistry, Graduate School of Engineering, Kyoto University, Katsura, Nishikyo-ku, Kyoto, Japan.
authors.forename: Shinya
authors.initials: S
authors.lastname: Tsukiji
authors.forename: Masayoshi
authors.initials: M
authors.lastname: Miyagawa
authors.forename: Yousuke
authors.initials: Y
authors.lastname: Takaoka
authors.forename: Tomonori
authors.initials: T
authors.lastname: Tamura
authors.forename: Itaru
authors.initials: I
authors.lastname: Hamachi