Sonodynamic effects of lomefloxacin derivatives conjugated with methoxy polyethylene glycol on sarcoma 180 cells.
BACKGROUND
Sonodynamic therapy (SDT) is a promising methodology for cancer treatment. Lomefloxacin hydrochloride (LFLX) has been reported to have sonodymamic antitumor effects.


METHODS
We synthesized LFLX derivatives conjugated with methoxy polyethylene glycol (PEGylated LFLXs) and investigated their ultrasonically induced antitumor effects.


RESULTS
After ultrasound exposure at 2.0 MHz for 30 s, the survival rates of Sarcoma 180 cells in the presence of lower molecular weight PEGylated LFLXs (200 microM) were significantly lower than those of the control and the LFLX at 1.5 and 2.0 W/cm2. This enhancement was significantly inhibited by the addition of L-histidine, but not by D-mannitol or superoxide dismutase. There was no apparent cell damage in the presence of high molecular weight PEGylated LFLX even at 3.0 W/cm2.


CONCLUSIONS
These findings indicate that the sonodynamic antitumor effects of lower molecular weight PEGylated LFLXs are better than those of LFLX.
Komori
Chiyo
C
Pharmaceutical Science, Akita University Hospital, Akita 010-8543, Japan.
Okada
Kyoji
K
Kawamura
Koichi
K
Suzuki
Norio
N
Chida
Shuichi
S
Suzuki
Toshio
T