Mass spectrometry-compatible silver staining of histones resolved on acetic acid-urea-Triton PAGE.
Acetic acid-Urea-Triton (AUT) PAGE is commonly used method to separate histone variants and their post-translationally modified forms. Coomassie staining is the preferred method for protein visualization; however, its sensitivity is less than that of silver staining. Though silver staining of histones in AUT-PAGE has been reported, the method is time-consuming, dependent on prior staining by Amido black and has not been reported suitable for mass spectrometry. Here, we propose 'SDS-Silver' method for rapid, sensitive and mass spectrometry-compatible staining of histones resolved on AUT-PAGE.
Pramod
Khare Satyajeet
KS
Cancer Research Institute, Advanced Center for Treatment Research and Education in Cancer, Tata Memorial Centre, Kharghar, Navi Mumbai, India.
Bharat
Khade
K
Sanjay
Gupta
G