title: B cells contribute to ischemia/reperfusion-mediated tissue injury.
abstract: Multiple elements are known to participate in ischemia/reperfusion (I/R)-mediated tissue injury. Amongst them, B cells have been shown to contribute by the production of antibodies that bind to ischemic cells and fix complement. It is currently unknown whether B cells participate through antibody-independent mechanisms in the pathogenesis of I/R. In a mesenteric I/R model we found that B cells infiltrate the injured intestine of normal and autoimmune mice 2h after reperfusion is established. B cell depletion protected mice from the development of I/R-mediated intestinal damage. The protection conferred by B cell depletion was significantly greater in MRL/lpr mice. Finally, we show that ischemic tissue expressed the B cell-attractant CXCL13 and infiltrating B cells expressed the corresponding receptor CXCR5. Our data grant B cells an antibody-independent role in the pathogenesis of intestinal I/R and suggest that B cells accumulate in the injured tissue in response to the chemokine CXCL13.
authors.affiliation: Division of Rheumatology, Beth Israel Deaconess Medical Center, Harvard Medical School, 330 Brookline Avenue, CLS-937, Boston, MA 02115, USA.
authors.forename: Jie
authors.initials: J
authors.lastname: Chen
authors.forename: José C
authors.initials: JC
authors.lastname: Crispín
authors.forename: Thomas F
authors.initials: TF
authors.lastname: Tedder
authors.forename: Jurandir
authors.initials: J
authors.lastname: Dalle Lucca
authors.forename: George C
authors.initials: GC
authors.lastname: Tsokos