Progesterone-induced blocking factor and cytokine profile in women with threatened pre-term delivery.
OBJECTIVE
The objective of this study was to compare serum concentrations of progesterone-induced blocking factor (PIBF), anti-inflammatory (IL-10),and pro-inflammatory (IL-6, TNFa, and IFNc) cytokines of women with threatened pre-term delivery, with those of women with normal pregnancy and to evaluate the impact of PIBF on the outcome of pregnancy.


METHODS
A prospective study was conducted on a sample of 30 women with threatened pre-term delivery (study group) and 20 healthy pregnant women (control group) between the 24th and 37th gestational weeks. Serum PIBF, anti-inflammatory (IL-10), and pro-inflammatory (IL-6, TNFa, and IFNc) cytokine concentrations were measured by enzyme-linked immunosorbent assay (ELISA).


RESULTS
Thirteen of 30 patients (43.3%) with symptoms of threatened pre-term delivery, and one of 20 patients (5%) in the control group delivered before the 37th week of gestation. Mean PIBF concentrations in serum samples of patients with threatened pre-term delivery were significantly lower than in those of healthy pregnant women (171.12 +/-162.06 ng/mL versus 272.85 +/- 114.87 ng/mL; P < 0.05).Women with symptoms of threatened pre-term delivery had significantly lower serum levels of IL-10, and higher levels of IL-6 as well as IFNc compared with healthy controls.


CONCLUSIONS
Our results indicate that measuring PIBF and cytokine concentrations in serum during pregnancy is feasible and may be important for understanding immunological causes of pre-term delivery.
Hudić
Igor
I
Clinic for Gynecology and Obstetrics, University Clinical Center, Trnovac bb, Tuzla, Bosnia and Herzegovina. i.hudic@bih.net.ba
Fatusić
Zlatan
Z
Szekeres-Bartho
Julia
J
Balić
Devleta
D
Polgar
Beáta
B
Ljuca
Dzenita
D
Dizdarević-Hudić
Larisa
L