title: HPLC analysis of lipoproteins in culture medium of hepatoma cells: an in vitro system for screening antihyperlipidemic drugs.
abstract: We isolated a HepG2-derived sub-clone (HepG2-Lipo), which possessed an increased lipoprotein synthesizing ability. HepG2-Lipo cells could secrete triglycerides (TG) and cholesterol at rates 9.4- and 6-fold higher, respectively, when compared to HepG2 cells. Real-time RT-PCR analysis revealed that the expression levels of sterol regulatory element-binding protein-1c and -2 were 2.9- and 1.5-fold higher than in HepG2 cells. Furthermore, two apolipoprotein (apo) genes (apoA-1 and apoB-100) in HepG2-Lipo cells were expressed at 2.8- and 1.9-fold higher levels when compared to those in parental cells. We examined the effects of three antihyperlipidemic agents on the lipoprotein profiles of HepG2-Lipo cells. Simvastatin at 5 microM selectively suppressed cholesterol secretion from HepG2-Lipo cells, and 500 microM fenofibrate inhibited both TG and cholesterol secretion from the cells.
authors.affiliation: Skylight Biotech Inc., 100-4 Sunada, Iijima, Akita, 010-0911, Japan.
authors.forename: Mizuho
authors.initials: M
authors.lastname: Itoh
authors.forename: Yukie
authors.initials: Y
authors.lastname: Abe
authors.forename: Yuka
authors.initials: Y
authors.lastname: Iwama
authors.forename: Fumiko
authors.initials: F
authors.lastname: Kimura
authors.forename: Mayumi
authors.initials: M
authors.lastname: Satoh
authors.forename: Mayumi
authors.initials: M
authors.lastname: Shoji
authors.forename: Junichiro
authors.initials: J
authors.lastname: Takahashi
authors.forename: Gen
authors.initials: G
authors.lastname: Toshima
authors.forename: Hiroki
authors.initials: H
authors.lastname: Sasaki
authors.forename: Kazuyuki
authors.initials: K
authors.lastname: Hiwatashi
authors.forename: Keishi
authors.initials: K
authors.lastname: Hata