Synthesis of conjugated spermine derivatives with 7-nitrobenzoxadiazole (NBD), rhodamine and bodipy as new fluorescent probes for the polyamine transport system.
The synthesis of a series of conjugated spermine derivatives with benzoxadiazole, phenylxanthene or bodipy fluorophores is described. These fluorescent probes were used to identify the activity of the polyamine transport system (PTS). N(1)-Methylspermine NBD conjugate 5 proved to have the optimal fluorescence characteristics and was used to show a selectivity for PTS-proficient CHO versus PTS-deficient CHO-MG cells. It can therefore be used as a tool for the selection of cells sensitive to cytotoxic compounds vectored through the PTS.
Guminski
Yves
Y
Division de Chimie Médicinale, Centre de Recherche Pierre Fabre, 17 rue Jean Moulin, 81106 Castres cedex, France.
Grousseaud
Martial
M
Cugnasse
Sandrine
S
Brel
Viviane
V
Annereau
Jean-Philippe
JP
Vispé
Stéphane
S
Guilbaud
Nicolas
N
Barret
Jean-Marc
JM
Bailly
Christian
C
Imbert
Thierry
T