title: Blocking the secretion of saliva by silencing the HlYkt6 gene in the tick Haemaphysalis longicornis.
abstract: Soluble N-ethylmaleimide-sensitive fusion protein attachment protein receptors (SNAREs) have been identified as the key components of the protein complexes that facilitate vesicle traffic, of which Ykt6 (from Saccharomyces cerevisiae, v-SNARE) is proved to be a multifunctional protein in the membrane fusion. In the present study, a tick homologue of Ykt6 (HlYkt6, predicted 22.6 kDa), was isolated from the ixodid tick Haemaphysalis longicornis. RT-PCR and Western blot analysis indicated that the gene and the encoded protein were expressed ubiquitously in different tissues of the partially fed adult tick. Silencing of the HlYkt6 gene resulted in a significant decrease of the engorged body weight (82.9 +/- 26.8 mg vs. 232.17 +/- 59.1 mg in the PBS-injected control group and 178.7 +/- 57.0 mg in the GFP dsRNA-injected control group) and high mortality of replete ticks (100% in tested group vs. 4.8% in the PBS and 20.4% in GFP dsRNA-injected control groups). Disruption of HlYkt6 mRNA led to the suppression of saliva secretion, and a lower anticoagulant activity of the released liquid from the glands (APTT time: 25.25 +/- 1.50 s) than that of the control groups (39.25 +/- 0.50 s in the PBS-treated group and 40.0 +/- 1.41 s in the GFP dsRNA-treated group). These results suggest the vital role of the HlYkt6 protein in the exocytosis of saliva proteins, the feeding and survival of ticks.
authors.affiliation: National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, Hokkaido, Japan.
authors.forename: Haiyan
authors.initials: H
authors.lastname: Gong
authors.forename: Rika
authors.initials: R
authors.lastname: Umemiya
authors.forename: Jinlin
authors.initials: J
authors.lastname: Zhou
authors.forename: Min
authors.initials: M
authors.lastname: Liao
authors.forename: Houshuang
authors.initials: H
authors.lastname: Zhang
authors.forename: Honglin
authors.initials: H
authors.lastname: Jia
authors.forename: Yoshifumi
authors.initials: Y
authors.lastname: Nishikawa
authors.forename: Xuenan
authors.initials: X
authors.lastname: Xuan
authors.forename: Kozo
authors.initials: K
authors.lastname: Fujisaki