title: [Effect of regenerated silk fibroin film on transcription and expression of vascular endothelial growth factor gene].
abstract: As a biomaterial to be used for reparation in the case of trauma, the silk fibroin, particularly its effect on the transcription and expression of VEGF gene, is a concern. In this study, the ECV304 cell's growth shape and growth curve on the regenerated silk fibroin film were observed, and its VEGF secretion level was measured by ELISA test. It was found that the regenerated silk fibroin film did not interfere with ECV304 cell's growth and function. The L929 cell transfected with human VEGF gene grew on the regenerated silk fibroin film; the real-time quantitative RT-PCR method and ELISA test were used for detecting the transcription and expression of VEGF gene. The results showed the regenerated silk fibroin film did not interfere with the transcription and expression of VEGF gene. Therefore, the regenerated silk fibroin film is a safe biomaterial for inducing vascularization with no untoward effect on the reparation of trauma.
authors.affiliation: Medical School, Soochow University, Suzhou 215123, China.
authors.forename: Quan
authors.initials: Q
authors.lastname: Huang
authors.forename: Jicheng
authors.initials: J
authors.lastname: Yang
authors.forename: Jingcheng
authors.initials: J
authors.lastname: Miao
authors.forename: Chunyu
authors.initials: C
authors.lastname: Liu
authors.forename: Yufeng
authors.initials: Y
authors.lastname: Xie
authors.forename: Weihua
authors.initials: W
authors.lastname: Sheng
authors.forename: Mingzhong
authors.initials: M
authors.lastname: Li