title: Testing the feasibility of intra-operative sentinel lymph node touch imprint cytology.
abstract: BACKGROUND
  Sentinel lymph node biopsy is emerging as the new standard for axillary staging in breast cancer. Intra-operative assessment of the sentinel lymph nodes allows immediate completion of axillary dissection during the same anaesthetic. This project was a quality assurance practice to establish feasibility, time-to-report, as well as accuracy of performing intra-operative assessment of sentinel lymph nodes using touch imprint cytology in our centre.
  
  
  METHODS
  This prospective audit included 146 sentinel lymph nodes from 74 consecutive patients with invasive breast cancer. All patients underwent axillary sentinel lymph node biopsy using combined blue dye and radiocolloid technique. Results of intra-operative touch imprint cytology using haematoxylin and eosin staining were compared with the definitive histopathology results.
  
  
  RESULTS
  Mean time to report touch imprint cytology was 25.7 +/- 6.4 min (range, 15-40 min). Histopathology demonstrated metastasis in 25 sentinel nodes from 17 (23%) patients. Intra-operative touch imprint cytology detected 15 nodes in 11 patients, giving a sensitivity of 60% (nodes) and 66.7% (patients) and specificity of 99.2% (nodes) and 98.2% (patients) based on the number of nodes and patients involved, respectively. Touch imprint cytology failed to show metastatic involvement in 10 nodes from 6 patients; of these, five nodes had micrometastasis (< 2 mm) and the other five had macrometastasis. One touch imprint cytology positive node contained isolated tumour cells only. Using intra-operative touch imprint cytology made a change in treatment of 11(14.9%) patients, and spared second axillary procedure in 7 (9.4%) patients.
  
  
  CONCLUSIONS
  Intra-operative sentinel lymph node assessment using touch imprint cytology is feasible within a busy NHS practice. We now offer touch imprint cytology to patients following appropriate counselling.
authors.affiliation: Renal Transplant Department, St George's Hospital, London, UK. alirezahamidian@yahoo.com
authors.forename: Alireza
authors.initials: A
authors.lastname: Hamidian Jahromi
authors.forename: Sankaran
authors.initials: S
authors.lastname: Narayanan
authors.forename: Fiona
authors.initials: F
authors.lastname: MacNeill
authors.forename: Peter
authors.initials: P
authors.lastname: Osin
authors.forename: Ashutash
authors.initials: A
authors.lastname: Nerurkar
authors.forename: Gerald
authors.initials: G
authors.lastname: Gui