title: Chemical and biomolecular characterization of Artemisia umbelliformis Lam., an important ingredient of the alpine liqueur "Genepi".
abstract: Artemisia umbelliformis Lam., an important alpine plant used for the preparation of flavored beverages, showed a remarkable intraspecific variability, at both genomic and gene product (secondary metabolites) levels. The variability of A. umbelliformis Lam. currently cultivated in Piedmont (Italy, Au1) and in Switzerland (Au2) was investigated by combining the chemical analysis of essential oil and sesquiterpene lactones and the molecular characterization of the 5S-rRNA-NTS gene by PCR and PCR-RFLP. Marked differences were observed between the two plants. Au1 essential oil contained alpha- and beta-thujones as the main components, whereas Au2 contained 1,8-cineole, borneol, and beta-pinene. Au1 sesquiterpene lactone fractions contained cis-8-eudesmanolide derivatives and Au2 the trans-6-germacranolide costunolide. Specific A. umbelliformis Au1 and Au2 primers were designed on the sequence of the 5S-rRNA gene spacer region. Furthermore, a PCR-restriction fragment length polymorphism (PCR-RFLP) method was applied using RsaI and TaqI restriction enzymes. Chemical and biomolecular data contributed to the characterization of A. umbeliformis chemotypes.
authors.affiliation: Dipartimento di Scienza e Tecnologia del Farmaco, Università di Torino, Via P. Giuria 9, I-10125 Torino, Italy. patrizia.rubiolo@unito.it
authors.forename: Patrizia
authors.initials: P
authors.lastname: Rubiolo
authors.forename: Maura
authors.initials: M
authors.lastname: Matteodo
authors.forename: Carlo
authors.initials: C
authors.lastname: Bicchi
authors.forename: Giovanni
authors.initials: G
authors.lastname: Appendino
authors.forename: Giorgio
authors.initials: G
authors.lastname: Gnavi
authors.forename: Cinzia
authors.initials: C
authors.lastname: Bertea
authors.forename: Massimo
authors.initials: M
authors.lastname: Maffei