title: Nesterenkonia alba sp. nov., an alkaliphilic actinobacterium isolated from the black liquor treatment system of a cotton pulp mill.
abstract: An alkaliphilic actinobacterium, designated strain CAAS 252(T), was isolated from the black liquor treatment system of a cotton pulp mill in Wuhan, China. Cells of strain CAAS 252(T) were Gram-positive, non-motile, non-endospore-forming, short rod-shaped, and grew optimally at 42 degrees C and pH 9-10 in the presence of 3 % (w/v) NaCl. Strain CAAS 252(T) contained MK-7, MK-8 and MK-9 as the major menaquinones and anteiso-C(17 : 0), anteiso-C(15 : 0) and C(16 : 0) as the predominant cellular fatty acids and had a peptidoglycan type of A4alpha, Lys-Gly-d-Asp. The DNA G+C content was 60.2 mol%. Based on analysis of 16S rRNA gene sequences (94.7-96.8 % similarity), DNA-DNA hybridization (<70 % relatedness) and chemotaxonomic characteristics, strain CAAS 252(T) belonged to the genus Nesterenkonia, but differed from all recognized species. Therefore, it is proposed that strain CAAS 252(T) represents a novel species of the genus Nesterenkonia, for which the name Nesterenkonia alba sp. nov. is proposed. The type strain is CAAS 252(T) (=CCTCC AB 207011(T)=DSM 19423(T)).
authors.affiliation: Key Laboratory for Feed Biotechnology of the Ministry of Agriculture, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, PR China.
authors.forename: Hui-Ying
authors.initials: HY
authors.lastname: Luo
authors.forename: Ya-Ru
authors.initials: YR
authors.lastname: Wang
authors.forename: Li-Hong
authors.initials: LH
authors.lastname: Miao
authors.forename: Pei-Long
authors.initials: PL
authors.lastname: Yang
authors.forename: Peng-Jun
authors.initials: PJ
authors.lastname: Shi
authors.forename: Cheng-Xiang
authors.initials: CX
authors.lastname: Fang
authors.forename: Bin
authors.initials: B
authors.lastname: Yao
authors.forename: Yun-Liu
authors.initials: YL
authors.lastname: Fan