title: Autoreactivity of serum immunoglobulin to periodontal tissue components: a pilot study.
abstract: BACKGROUND
  Periodontal diseases are inflammatory diseases resulting in the destruction of tissues of the periodontium. Although bacteria must be present for periodontal disease to occur, a susceptible host is also required, which is determined by genetic, environmental, and acquired factors. One such factor, autoimmunity, may play a role in the tissue destruction. Data indicate that some antibodies that occur in the gingival lesion are directed to host tissue components, such as type I collagen, although investigations of other periodontal autoimmune targets are limited.
  
  
  METHODS
  Histologic sections and extracts from periodontally healthy teeth and the associated soft tissues were probed with serum from localized aggressive periodontitis (LAgP), chronic periodontitis (CP), and periodontally healthy subjects to determine autoreactivity to components of the periodontium. Any autoreactivity observed was characterized further by mass spectrometry and enzyme-linked immunosorbent assay.
  
  
  RESULTS
  Autoreactivity to components of the periodontium was observed in CP and LAgP. Known autoimmune targets, such as collagen and heat shock protein, were identified along with multiple potential autoimmune targets, including members of the extracellular matrix, such as vimentin, spectrin, filamin, actin, lamin, keratin, and tubulin. Finally, it was determined that the autoreactivity observed in LAgP was more severe and diverse than that observed in CP.
  
  
  CONCLUSIONS
  These data demonstrated that autoimmune reactivity can play a role in the tissue destruction of periodontal disease but that the nature of the autoreactivity may differ based on the type and/or stage of periodontal disease.
authors.affiliation: Department of Periodontology, College of Dentistry, University of Florida, Gainesville, FL 32608, USA.
authors.forename: Theofilos
authors.initials: T
authors.lastname: Koutouzis
authors.forename: Derek
authors.initials: D
authors.lastname: Haber
authors.forename: Luciana
authors.initials: L
authors.lastname: Shaddox
authors.forename: Ikrammudin
authors.initials: I
authors.lastname: Aukhil
authors.forename: Shannon M
authors.initials: SM
authors.lastname: Wallet