CK2alpha phosphorylates BMAL1 to regulate the mammalian clock.
Clock proteins govern circadian physiology and their function is regulated by various mechanisms. Here we demonstrate that Casein kinase (CK)-2alpha phosphorylates the core circadian regulator BMAL1. Gene silencing of CK2alpha or mutation of the highly conserved CK2-phosphorylation site in BMAL1, Ser90, result in impaired nuclear BMAL1 accumulation and disruption of clock function. Notably, phosphorylation at Ser90 follows a rhythmic pattern. These findings reveal that CK2 is an essential regulator of the mammalian circadian system.
Tamaru
Teruya
T
Department of Physiology, Toho University School of Medicine, Tokyo, Japan.
Hirayama
Jun
J
Isojima
Yasushi
Y
Nagai
Katsuya
K
Norioka
Shigemi
S
Takamatsu
Ken
K
Sassone-Corsi
Paolo
P