title: Lysophospholipids stimulate prostate cancer cell migration via TRPV2 channel activation.
abstract: The physiological role, the mechanisms of activation, as well as the endogenous regulators for the non-selective cationic channel TRPV2 are not known so far. In the present work we report that endogenous lysophospholipids such as lysophosphatidylcholine (LPC) and lysophosphatidylinositol (LPI) induce a calcium influx via TRPV2 channel. This activation is dependent on the length of the side-chain and the nature of the lysophospholipid head-group. TRPV2-mediated calcium uptake stimulated by LPC and LPI occurred via Gq/Go-protein and phosphatidylinositol-3,4 kinase (PI3,4K) signalling. We have shown that the mechanism of TRPV2 activation induced by LPC and LPI is due to the TRPV2 channel translocation to the plasma membrane. The activation of TRPV2 channel by LPC and LPI leads to an increase in the cell migration of the prostate cancer cell line PC3. We have demonstrated that TRPV2 is directly involved in both steady-state and lysophospholipid-stimulated cancer cell migration. Thus, for the first time, we have identified one of the natural regulators of TRPV2 channel, one of the mechanisms of TRPV2 activation and regulation, as well as its pathophysiological role in cancer.
authors.affiliation: Inserm, U-800, Equipe labellisée par la Ligue Nationale contre le cancer, Villeneuve d'Ascq, F-59655 France; Université des Sciences et Technologies de Lille (USTL), Villeneuve d'Ascq, F-59655, France.
authors.forename: Michaël
authors.initials: M
authors.lastname: Monet
authors.forename: Dimitra
authors.initials: D
authors.lastname: Gkika
authors.forename: V'yacheslav
authors.initials: V
authors.lastname: Lehen'kyi
authors.forename: Albin
authors.initials: A
authors.lastname: Pourtier
authors.forename: Fabien
authors.initials: F
authors.lastname: Vanden Abeele
authors.forename: Gabriel
authors.initials: G
authors.lastname: Bidaux
authors.forename: Véronique
authors.initials: V
authors.lastname: Juvin
authors.forename: François
authors.initials: F
authors.lastname: Rassendren
authors.forename: Sandrine
authors.initials: S
authors.lastname: Humez
authors.forename: Natalia
authors.initials: N
authors.lastname: Prevarsakaya