title: Disruption of glycosylphosphatidylinositol-anchored lipid transfer protein gene altered cuticular lipid composition, increased plastoglobules, and enhanced susceptibility to infection by the fungal pathogen Alternaria brassicicola.
abstract: All aerial parts of vascular plants are covered with cuticular waxes, which are synthesized by extensive export of intracellular lipids from epidermal cells to the surface. Although it has been suggested that plant lipid transfer proteins (LTPs) are involved in cuticular lipid transport, the in planta evidence is still not clear. In this study, a glycosylphosphatidylinositol-anchored LTP (LTPG1) showing higher expression in epidermal peels of stems than in stems was identified from an Arabidopsis (Arabidopsis thaliana) genome-wide microarray analysis. The expression of LTPG1 was observed in various tissues, including the epidermis, stem cortex, vascular bundles, mesophyll cells, root tips, pollen, and early-developing seeds. LTPG1 was found to be localized in the plasma membrane. Disruption of the LTPG1 gene caused alterations of cuticular lipid composition, but no significant changes on total wax and cutin monomer loads were seen. The largest reduction (10 mass %) in the ltpg1 mutant was observed in the C29 alkane, which is the major component of cuticular waxes in the stems and siliques. The reduced content was overcome by increases of the C29 secondary alcohols and C29 ketone wax loads. The ultrastructure analysis of ltpg1 showed a more diffuse cuticular layer structure, protrusions of the cytoplasm into the vacuole in the epidermis, and an increase of plastoglobules in the stem cortex and leaf mesophyll cells. Furthermore, the ltpg1 mutant was more susceptible to infection by the fungus Alternaria brassicicola than the wild type. Taken together, these results indicated that LTPG1 contributed either directly or indirectly to cuticular lipid accumulation.
authors.affiliation: Department of Plant Biotechnology and Agricultural Plant Stress Research Center, College of Agriculture and Life Sciences, Chonnam National University, Gwangju 500-757, Republic of Korea.
authors.forename: Saet Buyl
authors.initials: SB
authors.lastname: Lee
authors.forename: Young Sam
authors.initials: YS
authors.lastname: Go
authors.forename: Hyun-Jong
authors.initials: HJ
authors.lastname: Bae
authors.forename: Jong Ho
authors.initials: JH
authors.lastname: Park
authors.forename: Sung Ho
authors.initials: SH
authors.lastname: Cho
authors.forename: Hong Joo
authors.initials: HJ
authors.lastname: Cho
authors.forename: Dong Sook
authors.initials: DS
authors.lastname: Lee
authors.forename: Ohkmae K
authors.initials: OK
authors.lastname: Park
authors.forename: Inhwan
authors.initials: I
authors.lastname: Hwang
authors.forename: Mi Chung
authors.initials: MC
authors.lastname: Suh