Sendai viral vector mediated angiopoietin-1 gene transfer for experimental ischemic limb disease.
Sendai virus vector is emerging as a promising vector for gene therapy, and angiopoietin-1 (Ang-1) has been reported to improve the blood flow recovery in the ischemic limb or heart. In this study, we constructed a human Ang-1-expressing Sendai viral vector (SeVhAng-1) and injected it into the ischemic limb of rats. We found that SeVhAng-1 improved the blood flow recovery and increased the capillary density of the ischemic limb, compared with the controls. We also found that SeVhAng-1 increased p-Akt during the early period of limb ischemia, and decreased apoptosis in ischemic limb. It suggests that SeVhAng-1 may serve as a potential therapeutic tool in ischemic limb disease.
Huang
Jianhua
J
Department of Molecular Medicine, Sapporo Medical University, Chuo-ku, Sapporo, Japan. hjhdr@hotmail.com
Inoue
Makoto
M
Hasegawa
Mamoru
M
Tomihara
Kei
K
Tanaka
Toshihiro
T
Chen
Jiahua
J
Hamada
Hirofumi
H