Ganoderma tsugae extract inhibits expression of epidermal growth factor receptor and angiogenesis in human epidermoid carcinoma cells: In vitro and in vivo.
We examined the anti-angiogenic effects of Ganoderma tsugae methanol extract (GTME) on human epidermoid carcinoma A-431 cells. Our data indicate that GTME inhibits the expression of epidermal growth factor receptor (EGFR) and vascular endothelial growth factor (VEGF) in vitro and in vivo, and also inhibits the capillary tube formation of human umbilical vein endothelial cells (HUVECs). We also show that the suppression of VEGF expression by GTME can be restored by treatment with EGF. These results suggest that GTME inhibits VEGF expression via the suppression of EGFR expression, resulting in the downregulation of VEGF secretion from epidermoid carcinoma A-431 cells. These findings reveal a novel role for G. tsugae in inhibiting EGFR and VEGF expression, which are important for tumor angiogenesis and growth. Thus, GTME may provide a potential therapeutic approach for anti-tumor treatment.
Hsu
Shih-Chung
SC
Graduate Institute of Medical Sciences, Taipei Medical University, Taiwan, ROC.
Ou
Chien-Chih
CC
Chuang
Tzu-Chao
TC
Li
Jhy-Wei
JW
Lee
Yi-Jen
YJ
Wang
Vinchi
V
Liu
Jah-Yao
JY
Chen
Chin-Shiang
CS
Lin
Song-Chow
SC
Kao
Ming-Ching
MC