title: Optimization of culture conditions for production of yeast biomass using bamboo wastewater by response surface methodology.
abstract: Response surface methodology (RSM) was applied to optimize culture conditions for the growth of Candida utilis with bamboo wastewater. A significant influence of initial pH, fermentation time and yeast extract on biomass of C. utilis was evaluated by Plackett-Burman design (PBD). These factors were further optimized using a central composite design (CCD) and RSM. A combination of initial pH 6.1, fermentation time 69 h and yeast extract 1.17 g/L was optimum for maximum biomass of C. utilis. A 1.7-fold enhancement of biomass of C. utilis was gained after optimization in shake-flask cultivation. The biomass of C. utilis reached 19.17 g/L in 3 L fermentor.
authors.affiliation: Key Laboratory of Forest Genetics and Biotechnology, Ministry of Education, Nanjing Forestry University, Nanjing, Jiangsu Province 210037, China. xin.lee@163.com
authors.forename: Xin
authors.initials: X
authors.lastname: Li
authors.forename: Jia
authors.initials: J
authors.lastname: Ouyang
authors.forename: Yong
authors.initials: Y
authors.lastname: Xu
authors.forename: Mu
authors.initials: M
authors.lastname: Chen
authors.forename: Xiangyang
authors.initials: X
authors.lastname: Song
authors.forename: Qiang
authors.initials: Q
authors.lastname: Yong
authors.forename: Shiyuan
authors.initials: S
authors.lastname: Yu