title: Extraction of glycyrrhizic acid and glabridin from licorice.
abstract: The extraction and separation conditions of glycyrrhizic acid and glabridin from licorice were investigated. By changing the different extraction solvents, procedures, times and temperature, the optimum extraction condition was established: the used of ethanol/water (30:70, v/v) as an extraction solvent, and 60 min dipping time under 50 degrees C. The extracts of licorice were separated and determined by reversed-phase high performance liquid chromatography with a methanol/water (70:30, v/v, containing 1% acetic acid) as the mobile phase. Under the optimum extraction condition, 2.39 mg/g of glycyrrhizic acid and 0.92 mg/g of glabridin were extracted from Chinese licorice and the recoveries were 89.7% and 72.5% respectively.
authors.affiliation: Center for Advanced Bioseperation Technology, Department of Chemical Engineering, Inha University, Incheon 402-751, Korea.
authors.forename: Minglei
authors.initials: M
authors.lastname: Tian
authors.affiliation: Center for Advanced Bioseperation Technology, Department of Chemical Engineering, Inha University, Incheon 402-751, Korea.
authors.forename: Hongyuan
authors.initials: H
authors.lastname: Yan
authors.affiliation: Center for Advanced Bioseperation Technology, Department of Chemical Engineering, Inha University, Incheon 402-751, Korea.
authors.forename: Kyung Ho
authors.identifier: 0000-0002-2736-1338
authors.initials: KH
authors.lastname: Row