Analysis of non-covalent bioconjugation of colloidal nanoparticles by means of atomic force microscopy and data clustering.
Non-covalent modification of nanoparticles with biomolecules is frequently applied in the generation of hybrid nanomaterials. Herein, we report on the analysis of non-covalently assembled hybrid nanoparticles on surfaces, using AFM measurements and examination of the resulting topography data by means of statistical cluster analysis. Two different model systems are investigated. On the one hand, we apply the combined AFM-cluster analysis to CdSe/ZnS core/shell quantum dots, bioconjugated with varying amounts of the hexahistidine-tagged enhanced yellow fluorescent protein (EYFP). On the other hand, gold nanoparticles modified with single-stranded DNA oligonucleotides were further functionalized by Watson-Crick base pairing, using complementary DNA oligonucleotides or covalent conjugates of DNA and streptavidin or EYFP. The results clearly indicate that cluster analysis of AFM data allows for the identification of sub-populations in complex reaction mixtures. Therefore, this analytical approach is useful to elucidate bioconjugate species, formed in the course of non-covalent synthesis of nanoparticle-biomolecule hybrid systems.
Irrgang
Jessica
J
Biologisch-Chemische Mikrostrukturtechnik, Fakultät Chemie, Technische Universität Dortmund, Otto-Hahn-Str. 6, 44227 Dortmund, Germany.
Ksienczyk
Jacqueline
J
Lapiene
Vidmantas
V
Niemeyer
Christof M
CM