title: Full genomic analysis of a human group A rotavirus G9P[6] strain from Eastern India provides evidence for porcine-to-human interspecies transmission.
abstract: Deduced amino acid sequence and phylogenetic analyses of a group A rotavirus G9P[6] strain (designated as mcs/13-07), detected from a 3-year-old child in Eastern India, revealed a VP8* closely related to porcine P[6] strains (P[6] sublineage 1D), and the VP7 clustered with G9 lineage-III strains. To our knowledge, this is the first report of human P[6] strain clustering in sublineage Id. Thus, to further characterize the evolutionary diversity of strain mcs/13-07, all gene segments were analyzed. VP6 and NSP4 exhibited genetic relatedness to Wa-like human subgroup II strains, while VP1-3, NSP1-3 and NSP5 were closely related to porcine strains. Based on the new classification system of rotaviruses, mcs/13-07 revealed a G9-P[6]-I1-R1-C1-M1-A8-N1-T1-E1-H1 genotype with close similarity to human Wa-like and porcine Gottfried strains. Therefore, considering the porcine-like or porcine origin of multiple gene segments, it might be tempting to assume that strain mcs/13-07 represents a rare instance of whole-virus transmission from pig to human, after which the virus evolved with time. Alternatively, it is possible that strain mcs/13-07 resulted from multiple reassortment events involving human subgroup II and porcine P[6] strains. Nevertheless, detection of strain mcs/13-07 provides further evidence for complex interspecies transmission events, which are frequent in developing countries.
authors.affiliation: Division of Virology, National Institute of Cholera and Enteric Diseases, P-33 C.I.T. Road Scheme XM, Beliaghata, Kolkata 700010, West Bengal, India.
authors.forename: Anupam
authors.initials: A
authors.lastname: Mukherjee
authors.forename: Dipanjan
authors.initials: D
authors.lastname: Dutta
authors.forename: Souvik
authors.initials: S
authors.lastname: Ghosh
authors.forename: Parikshit
authors.initials: P
authors.lastname: Bagchi
authors.forename: Shiladitya
authors.initials: S
authors.lastname: Chattopadhyay
authors.forename: Shigeo
authors.initials: S
authors.lastname: Nagashima
authors.forename: Nobumichi
authors.initials: N
authors.lastname: Kobayashi
authors.forename: Phalguni
authors.initials: P
authors.lastname: Dutta
authors.forename: Triveni
authors.initials: T
authors.lastname: Krishnan
authors.forename: Trailokya Nath
authors.initials: TN
authors.lastname: Naik
authors.forename: Mamta
authors.initials: M
authors.lastname: Chawla-Sarkar