Examination of seminal stain by HPLC assay of phenolphthalein.
Quantitative high performance liquid chromatography (HPLC) to detect semen was investigated in this study. Briefly, 1cm of a gauze thread with a seminal stain was soaked in the reaction mixture (phenolphthalein diphosphate tetrasodium dissolved in acetate buffer) for 5-10 min, and the supernatant was analyzed by HPLC with a spectrophotometric detector. Phenolphthalein was liberated from the reagent in the presence of acid phosphatase, and the liberated phenolphthalein was detected objectively and was unaffected by blood contamination. Since liberation of phenolphthalein from the reagent occurred slightly in control negative samples, the cut-off value of the examination should be set at 1.0 microg/ml.
Yoshida
Manabu
M
Department of Legal Medicine, Kansai Medical University, Moriguchi 570-8506, Japan. yoshidm@takii.kmu.ac.jp
Akane
Atsushi
A
Mitani
Tomoaki
T
Kobayashi
Tetsuya
T
Okii
Yutaka
Y