Molecular-scale force measurement in a coiled-coil peptide dimer by electron spin resonance.
A new method for measuring forces between small protein domains based on double electron-electron resonance (DEER) spectroscopy is demonstrated using a model peptide derived from the alpha-helical coiled-coil leucine zipper of yeast transcriptional activator GCN4. The equilibrium distribution of distances between two nitroxide spin labels rigidly attached to the helices of the dimer was determined by DEER and yielded a closing force of 100 +/- 10 pN between monomers, in excellent agreement with theoretical predictions.
Gullà
Stefano V
SV
Department of Chemistry and Chemical Biology, Northeastern University, Boston, Massachusetts 02115, USA.
Sharma
Gaurav
G
Borbat
Peter
P
Freed
Jack H
JH
Ghimire
Harishchandra
H
Benedikt
Monica R
MR
Holt
Natasha L
NL
Lorigan
Gary A
GA
Rege
Kaushal
K
Mavroidis
Constantinos
C
Budil
David E
DE