Investigation on the protective effect of α-mannan against the DNA damage induced by aflatoxin B₁in mouse hepatocytes.
Aflatoxin B(1) is a contaminant of agricultural and dairy products that can be related to mutagenic and carcinogenic effects. In this report we explore the capacity of alpha-mannan (Man) to reduce the DNA damage induced by AFB(1) in mouse hepatocytes. For this purpose we applied the comet assay to groups of animals which were first administered Man (100, 400 and 700 mg/kg, respectively) and 20 min later 1.0 mg/kg of AFB(1). Liver cells were obtained at 4, 10, and 16 h after the chemical administration and examined. The results showed no protection of the damage induced by AFB(1) with the low dose of the polysaccharide, but they did reveal antigenotoxic activity exerted by the two high doses. In addition, we induced a co-crystallization between both compounds, determined their fusion points and analyzed the molecules by UV spectroscopy. The obtained data suggested the formation of a supramolecular complex between AFB(1) and Man.
Madrigal-Santillán
Eduardo
E
Laboratorio de Genética, Escuela Nacional de Ciencias Biológicas, I.P.N., Carpio y Plan de Ayala. Sto. Tomas, México D.F. Cp 11340, México.|Laboratorio de Farmacología, Instituto de Ciencias de la Salud, UAEH., Ex-Hacienda de la Concepción. Tilcuautla. Pachuca de Soto, Hgo. Cp 42080, México.
Morales-González
José Antonio
JA
0000-0002-5980-0980
Laboratorio de Farmacología, Instituto de Ciencias de la Salud, UAEH., Ex-Hacienda de la Concepción. Tilcuautla. Pachuca de Soto, Hgo. Cp 42080, México.
Sánchez-Gutiérrez
Manuel
M
0000-0003-0342-8080
Laboratorio de Farmacología, Instituto de Ciencias de la Salud, UAEH., Ex-Hacienda de la Concepción. Tilcuautla. Pachuca de Soto, Hgo. Cp 42080, México.
Reyes-Arellano
Alicia
A
Departamento de Química Orgánica, Escuela Nacional de Ciencias Biológicas, I.P.N., Carpio y Plan de Ayala. Sto. Tomas, México D.F. Cp 11340, México.
Madrigal-Bujaidar
Eduardo
E
Laboratorio de Genética, Escuela Nacional de Ciencias Biológicas, I.P.N., Carpio y Plan de Ayala. Sto. Tomas, México D.F. Cp 11340, México.