title: [Screening of serum protein biomarkers by surface-enhanced laser desorption and ionization with time-of-flight detection mass spectrometry in patients with mycosis fungoides].
abstract: OBJECTIVE
  To screen serum biomarkers in patients with mycosis fungoides (MF) using surface-enhanced laser desorption and ionization with time-of-flight detection mass spectrometry (SELDI-TOF-MS) technique.
  
  
  METHODS
  Serum was analyzed from 14 patients with MF and 17 controls using CM10 Protein-chip to capture serum proteins, followed by Biomarker Wizard software analysis.
  
  
  RESULTS
  In all specimens, about 131 protein peaks could be detected when the relative molecular weight ranged from 0 to 50 000. When comparing the protein fingerprint between these two groups, 14 differentially expressed protein peaks were found. By searching SWISS-PRO database, we found 7 670Da peaks accord with C-C motif chemokine 22.
  
  
  CONCLUSIONS
  SELDI-TOF-MS technique can be used for screening serum protein biomarkers in patients with MF.
authors.affiliation: Department of Dermatology, PUMC Hospital, CAMS and PUMC, Beijing 100730, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Liu
authors.forename: Yan-Hua
authors.initials: YH
authors.lastname: Zheng
authors.forename: Jian-Zhong
authors.initials: JZ
authors.lastname: Zhang
authors.forename: Bao-Xi
authors.initials: BX
authors.lastname: Wang