title: Development of multiplex polymerase chain reaction for detection of Ehrlichia canis, Babesia spp and Hepatozoon canis in canine blood.
abstract: A multiplex polymerase chain reaction (PCR) has been developed for simultaneous detection of canine blood parasites, Ehrlichia canis, Babesia spp and Hepatozoon canis, from blood samples in a single reaction. The multiplex PCR primers were specific to E. canis VirB9, Babesia spp 16S rRNA and H. canis 16S rRNA genes. Specificity of the amplicons was confirmed by DNA sequencing. The assay was evaluated using normal canine and infected blood samples, which were detected by microscopic examination. This multiplex PCR offers scope for simultaneous detection of three important canine blood parasites and should be valuable in monitoring parasite infections in dogs and ticks.
authors.affiliation: Faculty of Veterinary Science, Mahidol University, Salaya, Nakhon Pathom, Thailand.
authors.forename: Kan
authors.initials: K
authors.lastname: Kledmanee
authors.forename: Sarin
authors.initials: S
authors.lastname: Suwanpakdee
authors.forename: Sakranmanee
authors.initials: S
authors.lastname: Krajangwong
authors.forename: Jarin
authors.initials: J
authors.lastname: Chatsiriwech
authors.forename: Parut
authors.initials: P
authors.lastname: Suksai
authors.forename: Pongpun
authors.initials: P
authors.lastname: Suwannachat
authors.forename: Ladawan
authors.initials: L
authors.lastname: Sariya
authors.forename: Ruangrat
authors.initials: R
authors.lastname: Buddhirongawatr
authors.forename: Phingphol
authors.initials: P
authors.lastname: Charoonrut
authors.forename: Kridsada
authors.initials: K
authors.lastname: Chaichoun