title: FSH receptor, KL1/2, P450, and PAPP genes in granulosa-lutein cells from in vitro fertilization patients show a different expression pattern depending on the infertility diagnosis.
abstract: OBJECTIVE
  To study the expression of mRNA for Kit ligand 1 (KL1), KL2, FSH receptor (FSHR), pregnancy-associated plasma protein (PAPP) and P450 in granulosa-lutein cells from IVF patients and its relationship with the infertility diagnosis and IVF outcome.
  
  
  METHODS
  In vitro assays.
  
  
  METHODS
  University laboratory and private IVF center.
  
  
  METHODS
  A total of 113 women undergoing IVF.
  
  
  METHODS
  Quantitative reverse-transcription polymerase chain reaction in granulosa-lutein cells from pooled follicles.
  
  
  METHODS
  Expression of mRNA for KL1, KL2, FSHR, PAPP and P450 in infertility patients with different infertility diagnoses.
  
  
  RESULTS
  Infertile patients with healthy ovaries show positive correlations among KL1, KL2, FSHR, PAPP, and P450 gene expression levels. In patients with ovarian disease, the correlation between KL1 and KL2 gene expression is maintained, but correlations between KL1/KL2 and FSHR, PAPP and P450 are not present except for KL1/FSHR in endometriosis and KL2/P450 in polycystic ovary syndrome. FSHR/KL1 and FSHR/KL2 expression correlates positively only in women who became pregnant.
  
  
  CONCLUSIONS
  Findings in healthy human ovaries agree with the feedback model of bone morphogenetic protein 15-KL1/KL2 cross-talk between oocyte and granulosa cells described in other species. The complex relationship between the expression of these genes seems to be disrupted in patients with infertility of ovarian origin. A normal pattern of gene expression and feedback seems to be associated with pregnancy.
authors.affiliation: Laboratorio Biología del Desarrollo, Departamento de Bioquímica y Biología Molecular, Universidad de la Laguna, La Laguna, Tenerife, Spain.
authors.forename: Rebeca
authors.initials: R
authors.lastname: González-Fernández
authors.forename: Oscar
authors.initials: O
authors.lastname: Peña
authors.forename: Jairo
authors.initials: J
authors.lastname: Hernández
authors.forename: Pablo
authors.initials: P
authors.lastname: Martín-Vasallo
authors.forename: Angela
authors.initials: A
authors.lastname: Palumbo
authors.forename: Julio
authors.initials: J
authors.lastname: Avila