title: Isolation, characterization and molecular cloning of new antimicrobial peptides  belonging to the brevinin-1 and temporin families from the skin of Hylarana  latouchii (Anura: Ranidae).
abstract: Around 40 species of Hylarana amphibians are distributed in tropical and subtropical Asia, and Chinese broad-folded frog, Hylarana latouchii (Boulenger, 1899) is one of them. In this study, six different cDNAs encoding four novel antimicrobial peptide precursors were cloned by screening the cDNA library of the Chinese broad-folded frog skin. The protein sequence analysis demonstrated that two deduced peptides belong to the brevinin-1 family, and the other two belong to temporin family of amphibian antimicrobial peptides. Thus, they were named as brevinin-1LT1 (FMGSALRIAAKVLPAALCQIFKKC), brevinin-1LT2 (FFGSVLKVAAKVLPAALCQIFKKC), temporin-LT1 (FLPGLIAGIAKML-NH2) and temporin-LT2 (FLPIALKALGSIFPKIL-NH2), respectively. Furthermore, brevinin-1LT1 and temporin-LT1 were purified by HPLC from the skin secretion of H. latouchii. In this work, all the peptides kill microbes by membrane-disturbing mechanisms, and this procedure was visualized via scanning electron microscopy (SEM).
authors.affiliation: College of Life Sciences, Hebei Normal University, Yuhua Dong Road 113#, Shijiazhuang, Hebei 050016, PR China.
authors.forename: Hui
authors.initials: H
authors.lastname: Wang
authors.forename: Xiuwen
authors.initials: X
authors.lastname: Yan
authors.forename: Haining
authors.initials: H
authors.lastname: Yu
authors.forename: Yuhong
authors.initials: Y
authors.lastname: Hu
authors.forename: Zhijun
authors.initials: Z
authors.lastname: Yu
authors.forename: Hongyuan
authors.initials: H
authors.lastname: Zheng
authors.forename: Ze
authors.initials: Z
authors.lastname: Chen
authors.forename: Zhiping
authors.initials: Z
authors.lastname: Zhang
authors.forename: Jingze
authors.initials: J
authors.lastname: Liu