title: Purification, crystallization and preliminary X-ray analysis of NtdA, a putative pyridoxal phosphate-dependent aminotransferase from Bacillus subtilis.
abstract: NtdA is a putative sugar aminotransferase that is required for the synthesis of 3,3'-neotrehalosadiamine. The enzyme was purified to homogeneity by means of Ni(2+)-affinity chromatography and was crystallized using the microbatch method. X-ray diffraction data were collected from a single crystal to 2.3 A resolution at the Canadian Light Source (CLS). The crystals belonged to space group P2(1), with unit-cell parameters a = 50.3, b = 106.7, c = 96.7 A, beta = 96.2 degrees, and contained two molecules per asymmetric unit.
authors.affiliation: Department of Chemistry, University of Saskatchewan, Saskatoon, Saskatchewan, Canada.
authors.forename: K E
authors.initials: KE
authors.lastname: van Straaten
authors.forename: D M
authors.initials: DM
authors.lastname: Langill
authors.forename: D R J
authors.initials: DR
authors.lastname: Palmer
authors.forename: D A R
authors.initials: DA
authors.lastname: Sanders