title: Docking simulations and in vitro assay unveil potent inhibitory action of papaverine against protein tyrosine phosphatase 1B.
abstract: The structural similarity between papaverine and berberine, a known inhibitor of human protein tyrosine phosphatase 1B (h-PTP 1B), prompted us to investigate the potential of papaverine as h-PTP 1B inhibitor. The investigation included simulated docking experiments to fit papaverine into the binding pocket of h-PTP 1B. Papaverine was found to readily dock within the binding pocket of h-PTP 1B in a low energy orientation via an optimal set of attractive interactions. Experimentally, papaverine illustrated potent in vitro inhibitory effect against recombinant h-PTP 1B (IC(50)=1.20 microM). In vivo, papaverine significantly decreased fasting blood glucose level of Balb/c mice. Our findings should encourage screening of other natural alkaloids for possible anti-h-PTP 1B activities.
authors.affiliation: Faculty of Pharmacy, University of Jordan.
authors.forename: Yasser
authors.initials: Y
authors.lastname: Bustanji
authors.forename: Mutasem Omar
authors.initials: MO
authors.lastname: Taha
authors.forename: Ihab Mustafa
authors.initials: IM
authors.lastname: Al-Masri
authors.forename: Mohammad Khalil
authors.initials: MK
authors.lastname: Mohammad