title: Transcriptional diversity and allelic variation in nicotinic acetylcholine receptor subunits of the red flour beetle, Tribolium castaneum.
abstract: Sequence analysis of 168 cDNA clones encoding 12 nicotinic acetylcholine receptor subunits, Tcasalpha1-Tcasalpha11 and Tcasbeta1, from the red flour beetle, Tribolium castaneum, revealed extensive post-transcriptional modification and multiple alleles. The greatest diversity was found for Tcasa6, where 18 unique transcripts, as a result of alternative and optional exon usage, were seen. A novel alternative exon 8d was found in one Tcasalpha6 transcript. Tcasalpha5 transcripts did not contain previously reported exons 8-10. Six subunits had transcripts that contained unspliced introns, which introduced premature stop codons. Intron 3' splice site variants were seen at six intron boundaries across five subunits. A-to-I RNA editing was seen only in Tcasalpha6. Alleles were found for all subunit genes, except Tcasalpha1 and Tcasalpha10. Transcriptional and allelic diversity are discussed with respect to receptor function and potential interactions with insecticides.
authors.affiliation: Department of Entomology, Cornell University, Ithaca, NY 14853-0901, USA.
authors.forename: F D
authors.initials: FD
authors.lastname: Rinkevich
authors.forename: J G
authors.initials: JG
authors.lastname: Scott