title: Organization of extracellular matrix fibers within polyglycolic acid-polylactic acid scaffolds analyzed using X-ray synchrotron-radiation phase-contrast micro computed tomography.
abstract: Spatiotemporal organized patterns of cell surface-associated and extracellular matrix (ECM)-embedded molecules play important roles in the development and functioning of tissues. ECM proteins interact with the surface of bioscaffold polymers and influence material-driven control of cell differentiation., Using X-ray phase-contrast micro computed tomography (microCT), we visualized the three-dimensional (3D) image of ECM organization after in vitro seeding of bone marrow-derived human and murine mesenchymal stem cells (MSCs) induced to myogenic differentiation, labelled with iron oxide nanoparticles, and seeded onto polyglycolic acid-polylactic acid scaffolds. X-ray microCT enabled us to detect with high spatial resolution the 3D structural organization of ECM within the bioscaffold and how the presence of cells modified the construct arrangement. Species-specific differences between the matrix produced by human and murine cells were observed. In conclusion, X-ray synchrotron radiation microCT analysis appeared to be a useful tool to identify the spatiotemporal pattern of organization of ECM fibers within a bioscaffold.
authors.affiliation: Dipartimento di Fisica e Ingegneria dei Materiali e del Territorio, Università Politecnica delle Marche, 60131 Ancona, Italy. gialbe@univpm.it
authors.forename: Gianni
authors.initials: G
authors.lastname: Albertini
authors.forename: Alessandra
authors.initials: A
authors.lastname: Giuliani
authors.forename: Vladimir
authors.initials: V
authors.lastname: Komlev
authors.forename: Francesca
authors.initials: F
authors.lastname: Moroncini
authors.forename: Armanda
authors.initials: A
authors.lastname: Pugnaloni
authors.forename: Giuseppina
authors.initials: G
authors.lastname: Pennesi
authors.forename: Marzia
authors.initials: M
authors.lastname: Belicchi
authors.forename: Corrado
authors.initials: C
authors.lastname: Rubini
authors.forename: Franco
authors.initials: F
authors.lastname: Rustichelli
authors.forename: Roberta
authors.initials: R
authors.lastname: Tasso
authors.forename: Yvan
authors.initials: Y
authors.lastname: Torrente