title: Characterization of a MAGE-1-derived HLA-A24 epitope-specific CTL line from a Japanese metastatic melanoma patient.
abstract: A MAGE-1 HLA-A24 peptide-specific CTL line was characterized using a novel staining approach in the case of a metastatic melanoma patient who exhibited a remarkable clinical response in HLA-A24 peptide cocktail-pulsed dendritic cell (DCs) vaccine therapy. Briefly, pre- or post-vaccine peripheral blood mononuclear cells (PBMCs) from the vaccinated patient were stimulated several times with MAGE-1 A24 peptide-pulsed DCs and T2-A24 cells in vitro. Expanded MAGE-1 A24-specific CTL line was investigated in terms of immunological functions. The proportion of MAGE-1 A24 tetramer+ CTLs increased from 0.04% to 18.6%, and the absolute numbers of MAGE-1 tetramer+ CTLs increased up to 5,068-fold after stimulations. Expanded CTL line exhibited a strong cytotoxic activity against MAGE-1+ cancer cell line in the restriction of HLA. Finally, successful identification of MAGE-1 A24 peptide-specific T-cell receptor (TCR) cDNA from anti-TCR MoAbsorted CTL was obtained for the first time and the specific cytotoxicity in TCR gene-transduced naive T-cells was confirmed.
authors.affiliation: Immunotherapy Division, Shizuoka Cancer Center Research Institute, 1007 Shimonagakubo, Nagaizumi-cho, Sunto-gun, Shizuoka 411-8777, Japan. y.akiyama@scchr.jp
authors.forename: Yasuto
authors.initials: Y
authors.lastname: Akiyama
authors.forename: Kouji
authors.initials: K
authors.lastname: Maruyama
authors.forename: Sachiko
authors.initials: S
authors.lastname: Tai
authors.forename: Masaru
authors.initials: M
authors.lastname: Komiyama
authors.forename: Akira
authors.initials: A
authors.lastname: Iizuka
authors.forename: Masako
authors.initials: M
authors.lastname: Takikawa
authors.forename: Chie
authors.initials: C
authors.lastname: Ohshita
authors.forename: Akifumi
authors.initials: A
authors.lastname: Yamamoto
authors.forename: Naoya
authors.initials: N
authors.lastname: Yamazaki
authors.forename: Yoshio
authors.initials: Y
authors.lastname: Kiyohara
authors.forename: Ken
authors.initials: K
authors.lastname: Yamaguchi