title: Preliminary study of sperm chromatin characteristics of the brachyuran crab Maja brachydactyla. Histones and nucleosome-like structures in decapod crustacean sperm nuclei previously described without SNBPs.
abstract: An interesting characteristic of decapod crustacean sperm nuclei is that they do not contain highly packaged chromatin. In the present study we re-examine the presence of DNA-interacting proteins in sperm nuclei of the brachyuran Maja brachydactyla. Although previous reports have indicated that, unlike the majority of sperm cells, DNA of decapod sperm is not organized by basic proteins, in this work we show that: (1) histones are present in sperm of M. brachydactyla; (2) histones are associated with sperm DNA; (3) histone H3 appears in lower proportions than the other core histones, while histone H2B appears in higher proportions; and (4) histone H3 in sperm nuclei is acetylated. This work complements a previous study of sperm histones of Cancer pagurus and supports the suggestion that decapod crustacean sperm chromatin deserves further attention.
authors.affiliation: Department of Physiological Sciences II, University of Barcelona, Campus Belvitge, Pavelló de Govern, 4a-Planta, Hospitalet de Llobregat 08907, Barcelona, Spain. klkurt00@smumn.edu
authors.forename: K
authors.initials: K
authors.lastname: Kurtz
authors.forename: J
authors.initials: J
authors.lastname: Ausió
authors.forename: M
authors.initials: M
authors.lastname: Chiva