title: Selective antegrade cerebral perfusion at two different temperatures compared to hypothermic circulatory arrest--an experimental study in the pig with microdialysis.
abstract: Hypothermic arrest and selective antegrade cerebral perfusion (SACP) is widely used during aortic arch surgery. The microdialysis technique monitors biomarkers of cellular metabolism and cellular integrity over time. In this study, the cerebral changes during hypothermic circulatory arrest (HCA) at 20 degrees C and HCA with SACP at two different temperatures, 20 and 28 degrees C, were monitored. Twenty-three pigs were divided into three groups. A microdialysis probe was fixated into the forebrain. Circulatory arrest started at a brain and body temperature of 20 degrees C or 28 degrees C. Arrest with/without cerebral perfusion (flow 10 ml/kg, max carotid artery pressure 70 mmHg) lasted for 80 min followed by reperfusion and rewarming during 40 min and an observation period of 120 min. The microdialysis markers were registered at six time-points. The lactate/pyruvate ratio (L/P ratio) and the lactate/glucose ratio (L/G ratio) increased significantly (P<0.05), during arrest, in the HCA group. The largest increase of glycerol was found in the group with tepid cerebral perfusion (28 degrees C) and the HCA group (P<0.05). This study supports the use of SACP over arrest. It also suggests that cerebral metabolism and cellular membrane integrity may be better preserved with SACP at 20 degrees C compared to 28 degrees C.
authors.affiliation: Department of Surgical Sciences, Thoracic Surgery, Uppsala University Hospital, Uppsala University, Uppsala, Sweden. ove_jonsson@spray.se
authors.forename: Ove
authors.initials: O
authors.lastname: Jonsson
authors.forename: Gunnar
authors.initials: G
authors.lastname: Myrdal
authors.forename: Vitas
authors.initials: V
authors.lastname: Zemgulis
authors.forename: Johann
authors.initials: J
authors.lastname: Valtysson
authors.forename: Lars
authors.initials: L
authors.lastname: Hillered
authors.forename: Stefan
authors.initials: S
authors.lastname: Thelin