Purification, crystallization and preliminary X-ray crystallographic analysis of xylose reductase from Candida tropicalis.
Xylose reductase (XR), which requires NADPH as a co-substrate, catalyzes the reduction of D-xylose to xylitol, which is the first step in the metabolism of D-xylose. The detailed three-dimensional structure of XR will provide a better understanding of the biological significance of XR in the efficient production of xylitol from biomass. XR of molecular mass 36.6 kDa from Candida tropicalis was crystallized using the hanging-drop vapour-diffusion method. According to X-ray diffraction data from C. tropicalis XR crystals at 2.91 A resolution, the unit cell belongs to space group P3(1) or P3(2). Preliminary analysis indicated the presence of four XR molecules in the asymmetric unit, with 68.0% solvent content.
Chen
Li Chun
LC
Scientific Research Division, National Synchrotron Radiation Research Center, Hsinchu 30076, Taiwan.
Huang
Sheng Cih
SC
Chuankhayan
Phimonphan
P
Chen
Chung Der
CD
Huang
Yen Chieh
YC
Jeyakanthan
Jeyaraman
J
Pang
Hsiao Fang
HF
Men
Lee Chung
LC
Chen
Yu Ching
YC
Wang
Yu Kuo
YK
Liu
Ming Yih
MY
Wu
Tung Kung
TK
Chen
Chun Jung
CJ