Imaging of laser-excited tissue autofluorescence bleaching rates.
Experimental methodology for imaging of laser-excited tissue autofluorescence bleaching rates has been developed and clinically tested. The fluorescence images were periodically captured from the same tissue area over a certain time, with subsequent detection of the fluorescence intensity decrease rate at each image pixel and further imaging the planar distribution of those values. Spectral features at each image pixel were analyzed with a hyperspectral imaging camera. Details of the equipment and image processing are described as well as some measurement results that confirm the feasibility of the proposed technology.
Spigulis
Janis
J
Bio-optics and Fiber Optics Laboratory, Institute of Atomic Physics and Spectroscopy, University of Latvia, Raina Boulevard 19, Riga LV-1586, Latvia. janispi@latnet.lv
Lihachev
Alexey
A
Erts
Renars
R