title: Rapid and accurate typing of Bordetella pertussis targeting genes encoding acellular vaccine antigens using real time PCR and High Resolution Melt analysis.
abstract: Real Time-PCR (RT-PCR) and high resolution melt (HRM) analyses were used for rapid typing of genes encoding components of the pertussis acellular vaccine, namely prn, ptxA, fhaB, fim2 and fim3. The length polymorphisms in prn were detected by RT-PCR followed by HRM; single nucleotide polymorphisms in prn and other genes were detected by hairpin primer RT-PCR. These rapid methods are suitable for large-scale studies of vaccine-driven evolution of Bordetella pertussis.
authors.affiliation: School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, NSW 2052, Australia.
authors.forename: Wai-Fong
authors.initials: WF
authors.lastname: Chan
authors.forename: Ram P
authors.initials: RP
authors.lastname: Maharjan
authors.forename: Peter R
authors.initials: PR
authors.lastname: Reeves
authors.forename: Vitali
authors.initials: V
authors.lastname: Sintchenko
authors.forename: Gwendolyn L
authors.initials: GL
authors.lastname: Gilbert
authors.forename: Ruiting
authors.initials: R
authors.lastname: Lan