Strategy for adapting wine yeasts for bioethanol production.
The Saccharomyces cerevisiae wine yeast strains 71B-1122 and K1-V1116 were used to derive strains that could tolerate and produce higher ethanol yields. Respiratory-deficient mutants resistant to 500 microg/mL lycorine were isolated. Two mutants, 71B-1122 YEBr L3 and K1-V1116 YEBr L4, were shown to achieve about 10% and 18% improvement in their glucose-to-ethanol conversion efficiency compared to their respective parent strains. The K1-V1116 YEBr L4 in particular can tolerate an ethanol yield of 18.8 +/- 0.8% at 3.5 weeks of fermentation and continued to consume most of the sugar until less than 1% glucose was left.
Ooi
Beng Guat
BG
Department of Chemistry, Middle Tennessee State University, P.O. Box 68, Murfreesboro, Tennessee, 37132, USA.
Lankford
Kevin R
KR
Department of Chemistry, Middle Tennessee State University, P.O. Box 68, Murfreesboro, Tennessee, 37132, USA.