title: Local upregulation of stromal cell-derived factor-1 after ligament injuries enhances homing rate of bone marrow stromal cells in rats.
abstract: In the present study the local expression of stromal cell-derived factor-1 (SDF-1) after ligament injury and correlated change in the homing rate of systemically induced bone marrow stromal cells (BMSCs) to the injured site were clarified using a rat medial collateral ligament (MCL) injury model. SDF-1 was temporarily upregulated peaking at 2 weeks after injury. Correlated with the alteration in SDF-1 expression, the homing rate of systemically induced BMSCs was temporarily upregulated peaking at 2-4 weeks after injury. The SDF-1 expression in the MCL seems to play a crucial role in cell homing. In addition, SDF-1 did not influence the BMSCs behavior in vitro in terms of the proliferation, adhesivity, and expression of ligament fibroblast markers. The cell-based therapy for ligament and tendon injury with reference to local expression of SDF-1 may be one of the available applications.
authors.affiliation: Department of Orthopaedic Surgery, Graduate School of Medicine, Hokkaido University , Sapporo, Japan.
authors.forename: Kazumi
authors.initials: K
authors.lastname: Shimode
authors.forename: Norimasa
authors.initials: N
authors.lastname: Iwasaki
authors.forename: Tokifumi
authors.initials: T
authors.lastname: Majima
authors.forename: Tadanao
authors.initials: T
authors.lastname: Funakoshi
authors.forename: Naohiro
authors.initials: N
authors.lastname: Sawaguchi
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Onodera
authors.forename: Akio
authors.initials: A
authors.lastname: Minami