title: Reprobing multicolor FISH preparations in lepidopteran chromosome.
abstract: Multicolor fluorescence in-situ hybridization (FISH) and subsequent reprobing of chromosome preparations increase the number of chromosomes and/or anchor loci on the chromosomes simultaneously identified. Reprobing techniques have been widely applied to chromosomes of vertebrates and plants. We have developed a novel reprobing protocol that utilizes multicolor FISH and bacterial artificial chromosome (BAC) probes to examine chromosome preparations in a model lepidopteran species, the silkworm, Bombyx mori. With standard two-color BAC-FISH, routinely used to map genes on B. mori chromosomes, we could localize only two probes on one preparation, whereas our new protocol combining five-color BAC-FISH and preparation reprobing enabled us to simultaneously map 10 probes, as demonstrated with the Bombyx Z chromosome. The improved BAC-FISH technique will facilitate karyotyping and synteny analysis in Lepidoptera.
authors.affiliation: Laboratory of Applied Molecular Entomology, Research Institute of Agriculture, Hokkaido University, Sapporo 060-8589, Japan.
authors.forename: Fukashi
authors.initials: F
authors.lastname: Shibata
authors.forename: Ken
authors.initials: K
authors.lastname: Sahara
authors.forename: Yota
authors.initials: Y
authors.lastname: Naito
authors.forename: Yuji
authors.initials: Y
authors.lastname: Yasukochi