title: Synthesis of conjugated spermine derivatives with 7-nitrobenzoxadiazole (NBD), rhodamine and bodipy as new fluorescent probes for the polyamine transport system.
abstract: The synthesis of a series of conjugated spermine derivatives with benzoxadiazole, phenylxanthene or bodipy fluorophores is described. These fluorescent probes were used to identify the activity of the polyamine transport system (PTS). N(1)-Methylspermine NBD conjugate 5 proved to have the optimal fluorescence characteristics and was used to show a selectivity for PTS-proficient CHO versus PTS-deficient CHO-MG cells. It can therefore be used as a tool for the selection of cells sensitive to cytotoxic compounds vectored through the PTS.
authors.affiliation: Division de Chimie Médicinale, Centre de Recherche Pierre Fabre, 17 rue Jean Moulin, 81106 Castres cedex, France.
authors.forename: Yves
authors.initials: Y
authors.lastname: Guminski
authors.forename: Martial
authors.initials: M
authors.lastname: Grousseaud
authors.forename: Sandrine
authors.initials: S
authors.lastname: Cugnasse
authors.forename: Viviane
authors.initials: V
authors.lastname: Brel
authors.forename: Jean-Philippe
authors.initials: JP
authors.lastname: Annereau
authors.forename: Stéphane
authors.initials: S
authors.lastname: Vispé
authors.forename: Nicolas
authors.initials: N
authors.lastname: Guilbaud
authors.forename: Jean-Marc
authors.initials: JM
authors.lastname: Barret
authors.forename: Christian
authors.initials: C
authors.lastname: Bailly
authors.forename: Thierry
authors.initials: T
authors.lastname: Imbert