title: Impact of a single amino acid in the variable region 2 of the Old World monkey TRIM5alpha SPRY (B30.2) domain on anti-human immunodeficiency virus type 2 activity.
abstract: Variable region 1 (V1) of the SPRY domain of TRIM5alpha is a major determinant for species-specific virus restriction in primates. We previously reported that a chimeric TRIM5alpha containing baboon V1 in the background of cynomolgus monkey TRIM5alpha showed potent anti-human immunodeficiency virus type 2 (HIV-2) activity. Since baboons are reportedly sensitive to HIV-2 infection, there was a discrepancy between the ability of baboon TRIM5alpha V1 to restrict HIV-2 and baboon sensitivity to HIV-2. In the study presented here, we examined the roles of V2 and V3 of the baboon TRIM5alpha SPRY domain in its anti-HIV-2 activity. A chimeric TRIM5alpha containing the entire baboon SPRY domain showed weak anti-HIV-2 activity. This attenuation of activity was caused by a single serine-to-proline substitution in baboon TRIM5alpha V2. These findings indicate that the combination of V1 with other variable regions of SPRY is important in anti-HIV-2 activity of primate TRIM5alpha.
authors.affiliation: Department of Viral Infections, Research Institute for Microbial Diseases, Osaka University, Suita-shi, Osaka, Japan.
authors.forename: Ken
authors.initials: K
authors.lastname: Kono
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Bozek
authors.forename: Francisco S
authors.initials: FS
authors.lastname: Domingues
authors.forename: Tatsuo
authors.initials: T
authors.lastname: Shioda
authors.forename: Emi E
authors.initials: EE
authors.lastname: Nakayama