Revised structure of the repeating unit of the O-specific polysaccharide from Azospirillum lipoferum strain SpBr17.
A neutral O-polysaccharide liberated by mild acid hydrolysis of a lipopolysaccharide isolated from Azospirillum lipoferum SpBr17 was investigated using 1D and 2D (1)H and (13)C NMR spectroscopy, including HSQC, HMBC, and NOESY as well as SDS-PAGE electrophoresis along with sugar and methylation analyses. The structure of the O-specific polysaccharide repeating unit was established as follows: [Formula: see text]. The presented structure is a revised version of the formula that was published earlier in the Abstracts of the 9th International Congress on Nitrogen Fixation in Cancun (Mexico, 1992).
Choma
Adam
A
Department of Genetics and Microbiology, Maria Curie-Sklodowska University, 19 Akademicka St., 20-033 Lublin, Poland. achoma@hektor.umcs.lublin.pl
Komaniecka
Iwona
I
Sowinski
Pawel
P