title: Disturbance of cell proliferation in response to mobile phone frequency radiation.
abstract: The aim of study was to determine the influence of mobile phone frequency radiation on the proliferation, cytoskeleton structure, and mitotic index of V79 cells after 1 h, 2 h, and 3 h of exposure. V79 cells were cultured in standard laboratory conditions and exposed to continuous-wave (CW) RF/MW radiation of 935 MHz, electric field strength of (8.2+/-0.3) V m(-1), and specific absorption rate (SAR) of 0.12 W kg(-1). To identify proliferation kinetics, the cells were counted for each hour of exposure 24 h, 48 h, 72 h, and 96 h after respective exposures. Microtubule proteins were determined using specific immunocytochemical methods. Cell smears were analysed under a fluorescent microscope. The study included negative and positive controls. Mitotic index was determined by estimating the number of dividing cells 24 h after exposure and dividing it with the total number of cells. In comparison to the controls, cell proliferation declined in cells exposed for three hours 72 h after irradiation (p < 0.05). Microtubule structure was clearly altered immediately after three hours of irradiation (p < 0.05). The mitotic index in RF/MW-exposed cells did not differ from negative controls. However, even if exposure did not affect the number of dividing cells, it may have slowed down cell division kinetics as a consequence of microtubule impairment immediately after exposure.
authors.affiliation: Institute for Medical Research and Occupational Health, Zagreb, Croatia. itrosic@imi.hr
authors.forename: Ivancica
authors.initials: I
authors.lastname: Trosić
authors.forename: Ivan
authors.initials: I
authors.lastname: Pavicić