Layer-by-layer assembled gold nanoparticles for siRNA delivery.
Although uptake into cells is highly complex and regulated, heterogeneous particle collectives are usually employed to deliver small interfering RNA (siRNA) to cells. Within these collectives, it is difficult to accurately identify the active species, and a decrease in efficacy is inherent to such preparations. Here, we demonstrate the manufacture of uniform nanoparticles with the deposition of siRNA on gold in a layer-by-layer approach, and we further report on the cellular delivery and siRNA activity as functions of surface properties.
Elbakry
Asmaa
A
Department of Pharmaceutical Technology, University of Regensburg, 93040 Regensburg, Germany.
Zaky
Alaa
A
Liebl
Renate
R
Rachel
Reinhard
R
Goepferich
Achim
A
Breunig
Miriam
M