title: Predicting virulence of Aeromonas isolates based on changes in transcription of c-jun and c-fos in human tissue culture cells.
abstract: OBJECTIVE
  To screen for the virulence potential of Aeromonas isolates based on the change in regulation of c-jun and c-fos in the human intestinal tissue culture cell line Caco-2.
  
  
  RESULTS
  Aeromonas cells were added to Caco-2 cells at a ratio of approx. 1 : 1. After 1-, 2- and 3-h incubation at 37 degrees C, mRNA was extracted from the cells and gene expression of two host genes, c-jun and c-fos, quantified. Aeromonas isolates which were pathogenic in the neonatal mouse model demonstrated up-regulation of c-jun and c-fos compared to avirulent isolates.
  
  
  CONCLUSIONS
  Human cell culture results showed that c-jun and c-fos were predictive of Aeromonas virulence.
  
  
  CONCLUSIONS
  An Aeromonas relative virulence scale is proposed for use in the testing of Aeromonas drinking water isolates.
authors.affiliation: USEPA, National Risk Management Research Laboratory, Water Supply/Water Resources Division, Cincinnati, OH 45268, USA.
authors.forename: S L
authors.initials: SL
authors.lastname: Hayes
authors.forename: M
authors.initials: M
authors.lastname: Waltmann
authors.forename: M
authors.initials: M
authors.lastname: Donohue
authors.forename: D J
authors.initials: DJ
authors.lastname: Lye
authors.forename: S J
authors.initials: SJ
authors.lastname: Vesper