title: High-sensitivity detection of fruit tree viruses using bacterial magnetic particles.
abstract: Prunus necrotic ring spot virus (PNRSV) and grapevine fanleaf virus (GFLV) were detected by fluoroimmunoassay using bacterial magnetic particles (BMPs), and a double antibody sandwich enzyme linked immunosorbent assay (DAS-ELISA). For the fluoroimmunoassay, fluorescein isothiocyanate labeled anti-PNRSV antibody or anti-GFLV antibody was conjugated onto BMPs of Magnetospirillum gryphiswaldense MSR-1. With this method, a very low minimum antigen concentration (1 x 10(6) dilution of the original sample concentration) could be detected. Using DAS-ELISA, the minimum antigen detection concentration was the original sample concentration. Thus, comparing these two methods, a BMP-based method could increase the sensitivity up to six orders of magnitude (10(6)) higher than an ELISA-based method of detection PNRSV and GFLV.
authors.affiliation: Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China.
authors.forename: Ji-Feng
authors.initials: JF
authors.lastname: Chen
authors.forename: Ying
authors.initials: Y
authors.lastname: Li
authors.forename: Zhen-Fang
authors.initials: ZF
authors.lastname: Wang
authors.forename: Ji-Lun
authors.initials: JL
authors.lastname: Li
authors.forename: Wei
authors.initials: W
authors.lastname: Jiang
authors.forename: Shao-Hua
authors.initials: SH
authors.lastname: Li