Ligand-directed tosyl chemistry for protein labeling in vivo.
Here we describe a method for the site-selective attachment of synthetic molecules into specific 'endogenous' proteins in vivo using ligand-directed tosyl (LDT) chemistry. This approach was applied not only for chemically labeling proteins in living cells, tissues and mice but also for constructing a biosensor directly inside cells without genetic engineering. These data establish LDT chemistry as a new tool for the study and manipulation of biological systems.
Tsukiji
Shinya
S
Department of Synthetic Chemistry and Biological Chemistry, Graduate School of Engineering, Kyoto University, Katsura, Nishikyo-ku, Kyoto, Japan.
Miyagawa
Masayoshi
M
Takaoka
Yousuke
Y
Tamura
Tomonori
T
Hamachi
Itaru
I