title: The up-regulation of large yellow croaker secretory IgM heavy chain at early phase of immune response.
abstract: An immunoglobulin M (IgM) heavy-chain gene homologue was isolated from the spleen cDNA library of the large yellow croaker Pseudosciaena crocea (LycIgH). The complete cDNA of LycIgH is 1,987 nucleotides long, encoding a protein of 585 amino acids with a putative molecular weight of 64.5 kDa. The deduced LycIgH possesses a typical secretory IgM heavy chain organization with a variable region (V(H)) connected to four constant regions (C(H1-4)) by a diversity segment (D(H)) and a joining segment (J(H)). Tissue expression profile analysis showed that LycIgH was constitutively expressed in gills, intestine, liver, kidney, heart, spleen, muscle, and blood, while at a higher level in spleen, kidney and intestine. Upon stimulation with poly (I: C), the LycIgH transcripts were quickly increased in spleen and kidney at 12 h post induction (with 5.87- and 5.48-fold mRNA increases, respectively), followed by a recovery to normal level at 24 h. The LycIgH transcripts in spleen and kidney induced by inactivated bacterial vaccine reached their peak levels at 48 h (14.53-fold) and 12 h (3.70-fold), respectively. These results indicated the up-regulation of LycIgH expression in spleen and kidney by poly (I: C) or bacterial vaccine occurred at the early phase of induction and was differentially modulated in the two tissues by different stimulations.
authors.affiliation: Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography, State Oceanic Administration, Xiamen, 361005, People's Republic of China.
authors.forename: Chen
authors.initials: C
authors.lastname: Tian
authors.affiliation: Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography, State Oceanic Administration, Xiamen, 361005, People's Republic of China. Chenxinh@tom.com.
authors.forename: Xinhua
authors.initials: X
authors.lastname: Chen
authors.affiliation: Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography, State Oceanic Administration, Xiamen, 361005, People's Republic of China. ajingqun2003@yahoo.com.cn.
authors.forename: Jingqun
authors.initials: J
authors.lastname: Ao