title: Local ATP generation by brain-type creatine kinase (CK-B) facilitates cell motility.
abstract: BACKGROUND
  Creatine Kinases (CK) catalyze the reversible transfer of high-energy phosphate groups between ATP and phosphocreatine, thereby playing a storage and distribution role in cellular energetics. Brain-type CK (CK-B) deficiency is coupled to loss of function in neural cell circuits, altered bone-remodeling by osteoclasts and complement-mediated phagocytotic activity of macrophages, processes sharing dependency on actomyosin dynamics.
  
  
  RESULTS
  Here, we provide evidence for direct coupling between CK-B and actomyosin activities in cortical microdomains of astrocytes and fibroblasts during spreading and migration. CK-B transiently accumulates in membrane ruffles and ablation of CK-B activity affects spreading and migration performance. Complementation experiments in CK-B-deficient fibroblasts, using new strategies to force protein relocalization from cytosol to cortical sites at membranes, confirmed the contribution of compartmentalized CK-B to cell morphogenetic dynamics.
  
  
  CONCLUSIONS
  Our results provide evidence that local cytoskeletal dynamics during cell motility is coupled to on-site availability of ATP generated by CK-B.
authors.affiliation: Department of Cell Biology, Nijmegen Centre for Molecular Life Sciences (NCMLS), Radboud University Nijmegen Medical Centre, Nijmegen, The Netherlands.
authors.forename: Jan W P
authors.initials: JW
authors.lastname: Kuiper
authors.forename: Remco
authors.initials: R
authors.lastname: van Horssen
authors.forename: Frank
authors.initials: F
authors.lastname: Oerlemans
authors.forename: Wilma
authors.initials: W
authors.lastname: Peters
authors.forename: Michiel M T
authors.initials: MM
authors.lastname: van Dommelen
authors.forename: Mariska M
authors.initials: MM
authors.lastname: te Lindert
authors.forename: Timo L M
authors.initials: TL
authors.lastname: ten Hagen
authors.forename: Edwin
authors.initials: E
authors.lastname: Janssen
authors.forename: Jack A M
authors.initials: JA
authors.lastname: Fransen
authors.forename: Bé
authors.initials: B
authors.lastname: Wieringa