title: Expression and purification of 30 kilodalton protein antigen of Ara- Burkholderia pseudomallei.
abstract: The 30 kDa protein of B. pseudomallei is found in virulent Ara- but not avirulent Ara+ strain. The gene was cloned in Escherichia coli JM105 employing pInIII-C2 vector. The open reading frame was 870 nucleotides with a guanine plus cytosine content of 69.9%. Arginine was the most abundant amino acid in the protein, having a PI of 12.65. Nucleotide sequence of the gene was 96% identical to B. pseudomallei 1710b chromosome II sequence CP000125.1, encoding an oxidoreductase of the short chain dehydrogenase/reductase family. The 30 kDa antigen was expressed as a maltose-fusion protein with a yield of 5.25 mg/l of bacterial culture.
authors.affiliation: Department of Immunology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand.
authors.forename: Utane
authors.initials: U
authors.lastname: Rungpanich
authors.forename: Supinya
authors.initials: S
authors.lastname: Pongsunk
authors.forename: Takayuki
authors.initials: T
authors.lastname: Ezaki
authors.forename: Pattama
authors.initials: P
authors.lastname: Ekpo