title: Chaperone-like effect of polyzwitterions on the interaction of C1q with IgG.
abstract: The amphiphilic polyzwitterion (PZ) poly(ethylene oxide-b-N,N-dimethyl(methacryloyloxyethyl)ammonium propanesulfonate), zwitterionic surfactant (ZS) n-dodecyl-N,N-dimethyl-3-ammonium-1-propanesulfonate, and zwitterionic monomer (ZM) N,N-dimethyl(methacryloyloxyethyl)ammonium propanesulfonate were analyzed for their suggested chaperone-like effect on the interaction of C1q and IgG. Our results proved that the PZ retarded the C1q interaction with IgG, demonstrating a specific protein-folding helper effect. The ZS enhanced this interaction, when the ZS concentration was lower than the critical micelle concentration (CMC), and retarded it, when the ZS concentration was above the CMC. The ZM, with no self-assembling ability, did not influence this interaction. These results support the hypothesis of a hydrophobic interaction between Pts and hydrophobic domains of partly denatured protein molecules. The amphiphilic self-assemblies, formed by polyzwitterionic macromolecules or zwitterionic surfactants, have the ability to transform the hydrophobic domains of the protein molecules into hydrophilic ones, covering them with their hydrophilic parts.
authors.affiliation: Sofia University, Faculty of Chemistry, 1 James Bouchier Aven., 1164 Sofia, Bulgaria.
authors.forename: Ivo
authors.initials: I
authors.lastname: Ivanov
authors.forename: Ivanka
authors.initials: I
authors.lastname: Tsacheva
authors.forename: Vishnia
authors.initials: V
authors.lastname: Stoyanova
authors.forename: Miroslav
authors.initials: M
authors.lastname: Nikolov
authors.forename: Magdalena I
authors.initials: MI
authors.lastname: Tchorbadjieva
authors.forename: Svetla
authors.initials: S
authors.lastname: Petrova
authors.forename: Latchezar
authors.initials: L
authors.lastname: Christov
authors.forename: Ventsislava
authors.initials: V
authors.lastname: Georgieva
authors.forename: George
authors.initials: G
authors.lastname: Georgiev