Separation of phenolic glycolipids in mycobacterium bovis BCG by reversed-phase high performance liquid chromatography.
A crude phenolic glycolipid extract from Mycobacterium bovis BCG was fractionated by column chromatography. A reversed-phase high performance liquid chromatography (HPLC) method with UV detection at 275 nm was developed for simultaneous detection and separation of phenolic glycolipids (PGLs) in Mycobacterium bovis BCG. This analysis provides a good resolution. Different solvent systems and columns for HPLC were compared. A system composed of acetonitrile-water in the ratio of 0-->80% at a flow rate of 0.8 mL/min and C8 analytical column were found to be optimum for HPLC of the phenolic glycolipids. This simple method is therefore appropriate to purify these compounds present in M. bovis extract.
Salimi
Mona
M
Research & Development Department, Pasteur Institute of Iran, Karaj, Iran.
Pourabdi
Ladan
L
Zakeri
Maryam
M
Abdipour
Nazanin
N