Glycosylation engineering of spinosyn analogues containing an L-olivose moiety.
Biosynthetic genes encoding proteins involved in the first steps of deoxyhexose biosynthesis from D-glucose-1-phosphate were expressed in Saccharopolyspora erythraea. The resulting mutant was able to accumulate and utilise TDP-L-olivose. Co-expression of the spinosyn glycosyl transferase SpnP in the resulting mutant endowed upon it the ability to biotransform exogenously added spinosyn aglycones to yield novel spinosyn analogues.
Gaisser
Sabine
S
Biotica Technology Ltd, Chesterford Research Park, Cambridge, UK CB10 1XL.
Carletti
Isabelle
I
Schell
Ursula
U
Graupner
Paul R
PR
Sparks
Thomas C
TC
Martin
Christine J
CJ
Wilkinson
Barrie
B