Purification of cytoplasmic actin by affinity chromatography using the C-terminal half of gelsolin.
A new rapid method of the cytoplasmic actin purification, not requiring the use of denaturants or high concentrations of salt, was developed, based on the affinity chromatography using the C-terminal half of gelsolin (G4-6), an actin filament severing and capping protein. When G4-6 expressed in Escherichia coli was added to the lysate of HeLa cells or insect cells infected with a baculovirus encoding the beta-actin gene, in the presence of Ca(2+) and incubated overnight at 4 degrees C, actin and G4-6 were both detected in the supernatant. Following the addition of Ni-Sepharose beads to the mixture, only actin was eluted from the Ni-NTA column by a Ca(2+)-chelating solution. The functionality of the cytoplasmic actins thus purified was confirmed by measuring the rate of actin polymerization, the gliding velocity of actin filaments in an in vitro motility assay on myosin V-HMM, and the ability to activate the ATPase activity of myosin V-S1.
Ohki
Takashi
T
Department of Physics, Waseda University, Okubo, Shinjuku-ku, Tokyo, Japan.
Ohno
Chikanori
C
Oyama
Kotaro
K
Mikhailenko
Sergey V
SV
Ishiwata
Shin'ichi
S