Isolation and identification of phlorotannins from Ecklonia stolonifera with antioxidant and anti-inflammatory properties.
Bioactivity-guided fractionation of Ecklonia stolonifera was used to determine the chemical identity of bioactive constituents, with potent antioxidant activities. The structures of the phlorotannins were determined on the basis of spectroscopic analysis, including NMR and mass spectrometry analysis. The antioxidant activities of the isolated compounds were evaluated by free radical scavenging activities in both in vitro and cellular systems. The anti-inflammatory effects of the isolated compounds were evaluated by determining their inhibitory effects on the production of nitric oxide (NO) and prostaglandin E(2) (PGE(2)) in lipopolysaccharide (LPS)-induced RAW 264.7 murine macrophage cells. The results indicated that phlorofucofuroeckol A, dieckol, and dioxinodehydroeckol showed potential radical scavenging activities against 2,2-diphenyl-1-picrylhydrazyl. Among them, phlorofucofuroeckol A and dieckol significantly suppressed the intracellular reactive oxygen species level assayed by 2',7'-dichlorofluorescein diacetate assay in LPS-induced RAW 264.7 cells. Phlorofucofuroeckol A significantly inhibited the LPS-induced production of NO and PGE(2) through the down-regulation of inducible nitric oxide synthase and cyclooxygenase 2 protein expressions. In conclusion, these results suggest that phlorofucofuroeckol A has a potential for functional foods with antioxidant and anti-inflammatory activities.
Kim
A-Reum
AR
Division of Food Science and Biotechnology, Pukyong National University, Busan 608-737.
Shin
Tai-Sun
TS
Lee
Min-Sup
MS
Park
Ji-Young
JY
Park
Kyoung-Eun
KE
Yoon
Na-Young
NY
Kim
Jong-Soon
JS
Choi
Jae-Sue
JS
Jang
Byeong-Churl
BC
Byun
Dae-Seok
DS
Park
Nam-Kyu
NK
Kim
Hyeung-Rak
HR