title: Dual production of poly(3-hydroxybutyrate) and glutamate using variable biotin concentrations in Corynebacterium glutamicum.
abstract: We previously synthesized poly(3-hydroxybutyrate) [P(3HB)] in recombinant Corynebacterium glutamicum, a prominent producer of amino acids. In this study, a two-step cultivation was established for the dual production of glutamate and P(3HB) due to the differences in the optimal concentration of biotin. Glutamate was extracellularly produced first under the biotin-limited condition of 0.3 microg/L. Production was then shifted to P(3HB) by addition of biotin to a total concentration of 9 microg/L. The final products obtained were 18 g/L glutamate and 36 wt% of P(3HB).
authors.affiliation: Division of Biotechnology and Macromolecular Chemistry, Graduate School of Engineering, Hokkaido University, N13 W8, Kita-ku, Sapporo-shi, Hokkaido, Japan.
authors.forename: Sung-Jin
authors.initials: SJ
authors.lastname: Jo
authors.forename: Chean Ring
authors.initials: CR
authors.lastname: Leong
authors.forename: Ken'ichiro
authors.initials: K
authors.lastname: Matsumoto
authors.forename: Seiichi
authors.initials: S
authors.lastname: Taguchi