Extraction of glycyrrhizic acid and glabridin from licorice.
The extraction and separation conditions of glycyrrhizic acid and glabridin from licorice were investigated. By changing the different extraction solvents, procedures, times and temperature, the optimum extraction condition was established: the used of ethanol/water (30:70, v/v) as an extraction solvent, and 60 min dipping time under 50 degrees C. The extracts of licorice were separated and determined by reversed-phase high performance liquid chromatography with a methanol/water (70:30, v/v, containing 1% acetic acid) as the mobile phase. Under the optimum extraction condition, 2.39 mg/g of glycyrrhizic acid and 0.92 mg/g of glabridin were extracted from Chinese licorice and the recoveries were 89.7% and 72.5% respectively.
Tian
Minglei
M
Center for Advanced Bioseperation Technology, Department of Chemical Engineering, Inha University, Incheon 402-751, Korea.
Yan
Hongyuan
H
Center for Advanced Bioseperation Technology, Department of Chemical Engineering, Inha University, Incheon 402-751, Korea.
Row
Kyung Ho
KH
0000-0002-2736-1338
Center for Advanced Bioseperation Technology, Department of Chemical Engineering, Inha University, Incheon 402-751, Korea.