title: Innovative electrochemical approach for an early detection of microRNAs.
abstract: The recent findings of circulating cell-free tissue specific microRNAs in the systemic circulation and the potential of their use as specific markers of disease highlight the need to make microRNAs testing a routine part of medical care. At the present time, microRNAs are detected by long and laborious techniques such as Northern blot, RT-PCR, and microarrays. The originality of our work consists in performing microRNAs detection through an electrochemical genosensor using a label-free method. We were able to directly detect microRNAs without the need of PCR and a labeling reaction. The test is simple, very fast and ultrasensitive, with a detection limit of 0.1 pmol. Particularly feasible for a routine microRNAs detection in serum and other biological samples, our technical approach would be of great scientific value and become a common method for simple miRNAs routine detection in both clinical and research settings.
authors.forename: E A
authors.initials: EA
authors.lastname: Lusi
authors.forename: M
authors.initials: M
authors.lastname: Passamano
authors.forename: P
authors.initials: P
authors.lastname: Guarascio
authors.forename: A
authors.initials: A
authors.lastname: Scarpa
authors.forename: L
authors.initials: L
authors.lastname: Schiavo