Regulation of adiponectin receptor 2 expression via PPAR-alpha in NIT-1 cells.
Adiponectin receptors mediate the antidiabetic effects of adiponectin. Although suggested to be mainly expressed in muscle, liver, and adipocyte cells, the expression of adiponectin receptors in beta cells is unclear. Given the primary involvement of this cell type in diabetes mellitus, we presently examined the expression level of adiponectin receptor 2 (AdiR2) in beta cells. Expression was significantly increased under acute hyperlipidemic conditions but impaired under chronic conditions. The impaired AdiR2 expression may play a role in worsened beta cell function. Clofibrate, an agonist of peroxisome proliferator-activated receptor-alpha (PPAR-alpha) delayed the palmitate-induced impairment of AdiR2 expression and PPAR-alpha; this delay was abolished by PPAR-alpha targeted small interfering RNA. The results suggest that AdiR2 expression is regulated by palmitate via PPAR-alpha.
Jung
Tae Woo
TW
Samsung Biomedical Institute, Seoul, Korea.
Lee
Myung Won
MW
Lee
Yong Jik
YJ
Kim
Seon Mee
SM
Lee
Kyoung Tae
KT
Whang
Wan Kyunn
WK
Cheon
Hwan Ju
HJ
Jeong
Yeon Taek
YT
Chung
Kun Wook
KW
Cho
Jae Min
JM
Kim
Do Hoon
DH
Jung
Tae Woo
TW