title: Survivin is not required for the endomitotic cell cycle of megakaryocytes.
abstract: Survivin is a member of the chromosome passenger complex, which plays an important role in chromosome alignment, separation, and cytokinesis. Although survivin is required for the proliferation and survival of hematopoietic stem and progenitor cells, the extent to which it is necessary for endomitosis of megakaryocytes remains controversial. To determine whether survivin is required for polyploidization, we analyzed mice with a megakaryocyte-specific deletion. PF4-Cre/survivin(fl/fl) mice harbored normal platelet counts with megakaryocytes that reached ploidy states comparable with those of control littermates. The CD41(+) cells within these animals showed little excision but increased annexin V staining, implying that survivin is required for survival of megakaryocyte progenitors in vivo. In contrast, megakaryocytes in which survivin was excised ex vivo showed robust excision and an increased degree of polyploidization. These results demonstrate that survivin is necessary for survival of megakaryocyte progenitors, but is not required for polyploidization of committed megakaryocytes.
authors.affiliation: Division of Hematology/Oncology, Northwestern University, Chicago, IL 60611, USA.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Wen
authors.forename: Cindy
authors.initials: C
authors.lastname: Leung
authors.forename: Zan
authors.initials: Z
authors.lastname: Huang
authors.forename: Sara
authors.initials: S
authors.lastname: Small
authors.forename: Alagarsamy Lakku
authors.initials: AL
authors.lastname: Reddi
authors.forename: Jonathan D
authors.initials: JD
authors.lastname: Licht
authors.forename: John D
authors.initials: JD
authors.lastname: Crispino