Effect of osteopontin on diarrhea duration and innate immunity in suckling mice infected with a murine rotavirus.
We studied the role of osteopontin (OPN) in host responses against rotavirus (RV) infection. OPN knockout (OPN-KO) suckling mice were more susceptible to RV (strain EW) infection and showed prolonged diarrhea duration compared to wild-type (WT) suckling mice. OPN in the small intestine of WT mice was expressed after 48 h post-infection. On day 2 postinfection, mRNA levels of interleukin-1beta, tumor necrosis factor-alpha, and interleukin-15 in OPN-KO mice were lower than in WT mice, although mRNA expression of Th-1- and Th-2-related cytokines in the small intestine were nearly the same between OPN-KO and WT mice. These results suggested that OPN is involved in innate responses against RV infection.
Maeno
Yoshimasa
Y
Department of Virology and Parasitology, Fujita Health University School of Medicine, Toyoake, Aichi, Japan. ymaeno@fujita-hu.ac.jp
Shinzato
Masanori
M
Nagashima
Shigeo
S
Rittling
Susan R
SR
Denhardt
David T
DT
Uede
Toshimitsu
T
Taniguchi
Koki
K