In vivo simultaneous monitoring of two fluorophores with lifetime contrast using a full-field time domain system.
Optical molecular imaging of small animals in vivo has witnessed dramatic growth during the past decade. Most commercial systems are based on continuous wave technology and measure solely bioluminescence or fluorescence intensity. Time domain (TD) technology enables the measurement of both intensity and fluorescence lifetime as an additional imaging metric. We have developed a novel, in-house, full-field TD system with dramatically faster acquisition times than available from a commercial TD system. Recent in vivo data from a mouse imaged with the full-field TD system has demonstrated the potential to monitor and discriminate two fluorophores injected simultaneously based on their fluorescence lifetime contrast.
Hall
David J
DJ
Department of Radiology, University of California, San Diego, 3855 Health Sciences Drive, La Jolla, California 92093, USA. djhall@ucsd.edu
Sunar
Ulas
U
Farshchi-Heydari
Salman
S
Han
Sung-Ho
SH