title: Hyperbranched PEI with various oligosaccharide architectures: synthesis, characterization, ATP complexation, and cellular uptake properties.
abstract: We present a rapid synthetic method for the development of hyperbranched PEIs decorated with different oligosaccharide architectures as carrier systems (CS) for drugs and bioactive molecules for in vitro and in vivo experiments. Reductive amination of hyperbranched PEI with readily available oligosaccharides results in sugar functionalized PEI cores with oligosaccharide shells of different densities. These core-shell architectures were characterized by NMR spectroscopy, elemental analysis, SLS, DLS, IR, and polyelectrolyte titration experiments. ATP complexation of theses polycations was examined by isothermal titration calorimetry to evaluate the binding energy and ATP/CS complexation ratios under physiological conditions. In vitro experiments showed an enhanced cellular uptake of ATP/CS complexes compared to those of the free ATP molecules. The results arise to initiate further noncovalent complexation studies of pharmacologically relevant molecules that may lead to the development of therapeutics based on this polymeric delivery platform.
authors.affiliation: Leibniz Institute of Polymer Research Dresden, Hohe Strasse 6, D-01069 Dresden, Germany. applhans@ipfdd.de
authors.forename: Dietmar
authors.initials: D
authors.lastname: Appelhans
authors.forename: Hartmut
authors.initials: H
authors.lastname: Komber
authors.forename: Mohiuddin Abdul
authors.initials: MA
authors.lastname: Quadir
authors.forename: Sven
authors.initials: S
authors.lastname: Richter
authors.forename: Simona
authors.initials: S
authors.lastname: Schwarz
authors.forename: Jereon
authors.initials: J
authors.lastname: van der Vlist
authors.forename: Achim
authors.initials: A
authors.lastname: Aigner
authors.forename: Martin
authors.initials: M
authors.lastname: Müller
authors.forename: Katja
authors.initials: K
authors.lastname: Loos
authors.forename: Jürgen
authors.initials: J
authors.lastname: Seidel
authors.forename: Karl-Friedrich
authors.initials: KF
authors.lastname: Arndt
authors.forename: Rainer
authors.initials: R
authors.lastname: Haag
authors.forename: Brigitte
authors.initials: B
authors.lastname: Voit