title: TFIIH and P-TEFb coordinate transcription with capping enzyme recruitment at specific genes in fission yeast.
abstract: Cyclin-dependent kinases (CDKs) are subunits of transcription factor (TF) IIH and positive transcription elongation factor b (P-TEFb). To define their functions, we mutated the TFIIH-associated kinase Mcs6 and P-TEFb homologs Cdk9 and Lsk1 of fission yeast, making them sensitive to inhibition by bulky purine analogs. Selective inhibition of Mcs6 or Cdk9 blocks cell division, alters RNA polymerase (Pol) II carboxyl-terminal domain (CTD) phosphorylation, and represses specific, overlapping subsets of transcripts. At a common target gene, both CDKs must be active for normal Pol II occupancy, and Spt5-a CDK substrate and regulator of elongation-accumulates disproportionately to Pol II when either kinase is inhibited. In contrast, Mcs6 activity is sufficient-and necessary-to recruit the Cdk9/Pcm1 (mRNA cap methyltransferase) complex. In vitro, phosphorylation of the CTD by Mcs6 stimulates subsequent phosphorylation by Cdk9. We propose that TFIIH primes the CTD and promotes recruitment of P-TEFb/Pcm1, serving to couple elongation and capping of select pre-mRNAs.
authors.affiliation: Molecular Biology Program, Memorial Sloan-Kettering Cancer Center, New York, NY 10065, USA.
authors.forename: Laia
authors.initials: L
authors.lastname: Viladevall
authors.forename: Courtney V
authors.initials: CV
authors.lastname: St Amour
authors.forename: Adam
authors.initials: A
authors.lastname: Rosebrock
authors.forename: Susanne
authors.initials: S
authors.lastname: Schneider
authors.forename: Chao
authors.initials: C
authors.lastname: Zhang
authors.forename: Jasmina J
authors.initials: JJ
authors.lastname: Allen
authors.forename: Kevan M
authors.initials: KM
authors.lastname: Shokat
authors.forename: Beate
authors.initials: B
authors.lastname: Schwer
authors.forename: Janet K
authors.initials: JK
authors.lastname: Leatherwood
authors.forename: Robert P
authors.initials: RP
authors.lastname: Fisher