title: Induction of natural killer T cell-dependent alloreactivity by administration of granulocyte colony-stimulating factor after bone marrow transplantation.
abstract: Granulocyte colony-stimulating factor (G-CSF) is often used to hasten neutrophil recovery after allogeneic bone marrow transplantation (BMT), but the clinical and immunological consequences evoked remain unclear. We examined the effect of G-CSF administration after transplantation in mouse models and found that exposure to either standard G-CSF or pegylated-G-CSF soon after BMT substantially increased graft-versus-host disease (GVHD). This effect was dependent on total body irradiation (TBI) rendering host dendritic cells (DCs) responsive to G-CSF by upregulating their expression of the G-CSF receptor. Stimulation of host DCs by G-CSF subsequently unleashed a cascade of events characterized by donor natural killer T cell (NKT cell) activation, interferon-gamma secretion and CD40-dependent amplification of donor cytotoxic T lymphocyte function during the effector phase of GVHD. Crucially, the detrimental effects of G-CSF were only present when it was administered after TBI conditioning and at a time when residual host antigen presenting cells were still present, perhaps explaining the conflicting and somewhat controversial clinical studies from the large European and North American BMT registries. These data have major implications for the use of G-CSF in disease states where NKT cell activation may have effects on outcome.
authors.affiliation: The Queensland Institute of Medical Research, Brisbane, Australia.
authors.forename: Edward S
authors.initials: ES
authors.lastname: Morris
authors.forename: Kelli P A
authors.initials: KP
authors.lastname: MacDonald
authors.forename: Rachel D
authors.initials: RD
authors.lastname: Kuns
authors.forename: Helen M
authors.initials: HM
authors.lastname: Morris
authors.forename: Tatjana
authors.initials: T
authors.lastname: Banovic
authors.forename: Alistair L J
authors.initials: AL
authors.lastname: Don
authors.forename: Vanessa
authors.initials: V
authors.lastname: Rowe
authors.forename: Yana A
authors.initials: YA
authors.lastname: Wilson
authors.forename: Neil C
authors.initials: NC
authors.lastname: Raffelt
authors.forename: Christian R
authors.initials: CR
authors.lastname: Engwerda
authors.forename: Angela C
authors.initials: AC
authors.lastname: Burman
authors.forename: Kate A
authors.initials: KA
authors.lastname: Markey
authors.forename: Dale I
authors.initials: DI
authors.lastname: Godfrey
authors.forename: Mark J
authors.initials: MJ
authors.lastname: Smyth
authors.forename: Geoffrey R
authors.initials: GR
authors.lastname: Hill