title: Protective effect of the ethanol extract of Magnolia officinalis and 4-O-methylhonokiol on scopolamine-induced memory impairment and the inhibition of acetylcholinesterase activity.
abstract: Magnolol, honokiol, and obovatol are well-known bioactive constituents of the bark of Magnolia officinalis and have been used as traditional Chinese medicines for the treatment of neurosis, anxiety, and stroke. We recently isolated novel active compound (named 4-O-methylhonokiol) from the ethanol extract of Magnolia officinalis. The present study aimed to test two different doses of ethanol extracts of Magnolia officinalis (5 and 10 mg/kg/mouse, p.o., 1 week) and 4-O-methylhonokiol (0.75 and 1.5 mg/kg/mouse, p.o., 1 week) administered for 7 days on memory impairment induced by scopolamine (1 mg/kg body weight i.p.) in mice. Memory and learning were evaluated using the Morris water maze and the step-down avoidance test. Both the ethanol extract of Magnolia officinalis and 4-O-methylhonokiol prevented memory impairment induced by scopolamine in a dose-dependent manner. The ethanol extract of Magnolia officinalis and 4-O-methylhonokiol also dose-dependently attenuated the scopolamine-induced increase of acetylcholinesterase (AChE) activity in the cortex and hippocampus of mice, and inhibited AChE activity in vitro with IC(50) (12 nM). This study, therefore, suggests that the ethanol extract of Magnolia officinalis and its major ingredient, 4-O-methylhonokiol, may be useful for the prevention of the development or progression of AD.
authors.affiliation: College of Pharmacy, Chungbuk National University, 12, Gaesin-dong, Heungduk-gu, Cheongju, Chungbuk, 361-763, Korea.
authors.forename: Yong Kyung
authors.initials: YK
authors.lastname: Lee
authors.forename: Dong Yeon
authors.initials: DY
authors.lastname: Yuk
authors.forename: Tae Il
authors.initials: TI
authors.lastname: Kim
authors.forename: Young Heui
authors.initials: YH
authors.lastname: Kim
authors.forename: Kyoung Tae
authors.initials: KT
authors.lastname: Kim
authors.forename: Ki Ho
authors.initials: KH
authors.lastname: Kim
authors.forename: Beom Jun
authors.initials: BJ
authors.lastname: Lee
authors.forename: Sang-Yoon
authors.initials: SY
authors.lastname: Nam
authors.forename: Jin Tae
authors.initials: JT
authors.lastname: Hong