Flexible low-cost system for small animal aerosol inhalation exposure to drugs, proteins, inflammatory agents, and infectious agents.
The design for a simple, low-cost aerosol generation system for rodent inhalation studies is described here. This system is appropriate for low biohazard-level agents. In this study, two biosafety level 2 agents, Pasturella pneumotropica and Pseudomonas aeruginosa, were tested successfully. This system was also used to immunize mice and guinea pigs in ovalbumin-based models of pulmonary inflammation. This design is appropriate for studies with limited budgets and lower-level biosafety containment.
Schroeder
William G
WG
Department of Microbiology, Immunology & Pathology, Colorado State University, Fort Collins, CO 80523, USA.
Mitrescu
Laura M
LM
Hart
Marcia L
ML
Unnithan
Ranjana
R
Gilchrist
John M
JM
Smith
Erin E
EE
Shanley
Crystal
C
Benedict
Katharine M
KM
Taraba
Lauren
L
Volckens
John
J
Basaraba
Randall J
RJ
Schenkel
Alan R
AR