Dual production of poly(3-hydroxybutyrate) and glutamate using variable biotin concentrations in Corynebacterium glutamicum.
We previously synthesized poly(3-hydroxybutyrate) [P(3HB)] in recombinant Corynebacterium glutamicum, a prominent producer of amino acids. In this study, a two-step cultivation was established for the dual production of glutamate and P(3HB) due to the differences in the optimal concentration of biotin. Glutamate was extracellularly produced first under the biotin-limited condition of 0.3 microg/L. Production was then shifted to P(3HB) by addition of biotin to a total concentration of 9 microg/L. The final products obtained were 18 g/L glutamate and 36 wt% of P(3HB).
Jo
Sung-Jin
SJ
Division of Biotechnology and Macromolecular Chemistry, Graduate School of Engineering, Hokkaido University, N13 W8, Kita-ku, Sapporo-shi, Hokkaido, Japan.
Leong
Chean Ring
CR
Matsumoto
Ken'ichiro
K
Taguchi
Seiichi
S