title: Yeast vitality determination based on intracellular NAD(P)H fluorescence measurement during aerobic-anaerobic transition.
abstract: This work presents a yeast-cell vitality-assessment method based on on-line intracellular fluorescence measurement. The intracellular NAD(P)H fluorescence of a cell suspension is recorded during transition from aerobic to anaerobic conditions and the output signal is evaluated as a measure of yeast vitality (quality). This fluorescence method showed a highly satisfactory correlation with even low dead cell numbers where the acidification power test could not be applied.
authors.affiliation: Department of Fermentation Chemistry and Bioengineering, Institute of Chemical Technology, Prague, Czech Republic.
authors.forename: M
authors.initials: M
authors.lastname: Kurec
authors.forename: G
authors.initials: G
authors.lastname: Kuncová
authors.forename: T
authors.initials: T
authors.lastname: Brányik