[Expression, characterization of recombinant human soluble BAFF secreted from CHO cell].
B cell-activating factor of the TNF family (BAFF) is critical for B cell maturation and survival. Here, we constructed a stable CHO cell line, in which the expression level of soluble form of BAFF (sBAFF) was raised from 0.13 microg/ml to 0.55 microg/ml. Purified recombinant sBAFF from these CHO cells not only bound to its receptors but also co-stimulated the proliferation of human peripheral blood B lymphocyte in vitro. These results provided us with a useful basis for further studies about sBAFF-related research.
Wu
H T
HT
Yan
X M
XM
Hu
Y L
YL
Diao
Z Y
ZY
Wu
J
J
Zhang
S Q
SQ