Mass spectrometry fingerprinting of media used for in vitro production of bovine embryos.
Using the bovine species as a biological model, direct infusion chip-based nano-electrospray ionization mass spectrometry (nano-ESI-MS) fingerprinting in the positive ion mode is used to obtain fast chemical profiles of media used for in vitro production of bovine embryos. Nano-ESI-MS fingerprinting is useful for characterization and routine quality control requiring no sample pre-separation, being able to differentiate four different media (IVM, IVF, SOF and HSOF) via principal component analysis (PCA). For media stored at +4 degrees C for up to 45 days, no significant (p>0.05) variation was observed in cleavage and blastocyst rate development, as well as in the nano-ESI-MS chemical profiles. For media exposed to a heat shock (60 degrees C for 3 h), no significant decrease (p>0.05) in embryo development rates was observed, but nano-ESI-MS profiles were quite distant from fresh control media in the PCA. For frozen media (-70 degrees C for 2 months), again no significant variation (p>0.05) in embryo development was noticed, but nano-ESI-MS profiles from all media were significantly affected. These results indicate that nano-ESI(+)-MS fingerprinting was able to characterize different media based on their specific chemical profile. The technique seems therefore applicable as a routine quality control assay, detecting, for example, compositional changes after temperature variations that may affect post-transfer embryo viability.
Ferreira
Christina Ramires
CR
In Vitro Brasil Ltda, Mogi Mirim-SP, 13800-970, Brazil. christina@iqm.unicamp.br
Souza
Gustavo Henrique Martins Ferreira
GH
Riccio
Maria Francesca
MF
Catharino
Rodrigo Ramos
RR
Pontes
José Henrique Fortes
JH
Basso
Andréa Cristina
AC
Júnior
José Carlos Ereno
JC
Perecin
Felipe
F
Eberlin
Marcos Nogueira
MN