title: Human hnRNP Q re-localizes to cytoplasmic granules upon PMA, thapsigargin, arsenite and heat-shock treatments.
abstract: Eukaryotic gene expression is regulated on different levels ranging from pre-mRNA processing to translation. One of the most characterized families of RNA-binding proteins is the group of hnRNPs: heterogenous nuclear ribonucleoproteins. Members of this protein family play important roles in gene expression control and mRNAs metabolism. In the cytoplasm, several hnRNPs proteins are involved in RNA-related processes and they can be frequently found in two specialized structures, known as GW-bodies (GWbs), previously known as processing bodies: PBs, and stress granules, which may be formed in response to specific stimuli. GWbs have been early reported to be involved in the mRNA decay process, acting as a site of mRNA degradation. In a similar way, stress granules (SGs) have been described as cytoplasmic aggregates, which contain accumulated mRNAs in cells under stress conditions and present reduced or inhibited translation. Here, we characterized the hnRNP Q localization after different stress conditions. hnRNP Q is a predominantly nuclear protein that exhibits a modular organization and several RNA-related functions. Our data suggest that the nuclear localization of hnRNP Q might be modified after different treatments, such as: PMA, thapsigargin, arsenite and heat shock. Under different stress conditions, hnRNP Q can fully co-localize with the endoplasmatic reticulum specific chaperone, BiP. However, under stress, this protein only co-localizes partially with the proteins: GW182-GWbs marker protein and TIA-1 stress granule component.
authors.affiliation: Brazilian Synchrotron Light Laboratory-LNLS, 13083-970 Campinas, SP, Brazil.
authors.forename: Alexandre J C
authors.initials: AJ
authors.lastname: Quaresma
authors.forename: G C
authors.initials: GC
authors.lastname: Bressan
authors.forename: L M
authors.initials: LM
authors.lastname: Gava
authors.forename: D C F
authors.initials: DC
authors.lastname: Lanza
authors.forename: C H I
authors.initials: CH
authors.lastname: Ramos
authors.forename: Jörg
authors.initials: J
authors.lastname: Kobarg