title: Eosinophils involved in fulminant hepatic failure are associated with high interleukin-6 expression and absence of interleukin-5 in liver and peripheral blood.
abstract: OBJECTIVE
  Although eosinophils are considered to play an important role in the pathogenesis of various parasitic, allergic and autoimmune digestive diseases, their role in fulminant hepatic failure (FHF) is unknown. Our contribution was to identify and quantify eosinophils and cytokine levels [interleukin (IL)-6, IL-5 and macrophage inflammatory protein (MIP)-1alpha] in liver parenchyma and peripheral blood from FHF patients at pre- and post-transplantation steps.
  
  
  METHODS
  Histochemical methods were used to identify/quantify eosinophils in liver samples. Liver and plasma cytokine levels were quantified using immunofluorescence methods.
  
  
  RESULTS
  Fulminant hepatic failure patients showed a high number of intrahepatic eosinophils concomitant with an increased expression of IL-6, besides the IL-6-positive eosinophils associated with the lack of IL-5. Also, an increased number of eosinophils and soluble IL-6 and MIP-1alpha with a low expression of IL-5 in peripheral blood at the pretransplantation step was observed.
  
  
  CONCLUSIONS
  The increased number of intrahepatic eosinophils, besides the high production of IL-6, may be involved in liver dysfunction. In addition, the low presence of IL-5 in liver and peripheral blood may represent a particular pattern of eosinophil behaviour in human liver failure, which may also involve MIP-1alpha. Further ex vivo studies are necessary to evaluate the specific role of eosinophils in FHF.
authors.affiliation: Laboratório de Desenvolvimento Tecnológico em Virologia - Instituto Oswaldo Cruz-Fiocruz, Rio de Janeiro, Brazil. dcsantos@ioc.fiocruz.br
authors.forename: Damião Carlos Moraes
authors.initials: DC
authors.lastname: dos Santos
authors.forename: José Manoel
authors.initials: JM
authors.lastname: da Silva Gomes Martinho
authors.forename: Lúcio Filgueiras
authors.initials: LF
authors.lastname: Pacheco-Moreira
authors.forename: Cristina
authors.initials: C
authors.lastname: Carvalho Viana de Araújo
authors.forename: Adriana
authors.initials: A
authors.lastname: Caroli-Bottino
authors.forename: Vera Lúcia
authors.initials: VL
authors.lastname: Pannain
authors.forename: Karen
authors.initials: K
authors.lastname: Soares Trinta
authors.forename: Mariana
authors.initials: M
authors.lastname: Gandini
authors.forename: Patrícia Cristina
authors.initials: PC
authors.lastname: da Costa Neves
authors.forename: Denise Cristina
authors.initials: DC
authors.lastname: de Souza Matos
authors.forename: Luzia Fátima
authors.initials: LF
authors.lastname: Gonçalves Caputo
authors.forename: Marcelo
authors.initials: M
authors.lastname: Pelajo-Machado
authors.forename: Marcelo
authors.initials: M
authors.lastname: Alves Pinto