Assessing the risk of skin damage due to femtosecond laser irradiation.
We irradiated freshly excised skin biopsies with four irradiation regimes usually taken for multiphoton imaging. If there is any skin damaging, it is mainly an effect similar to the damaging effects of UV-irradiation. Using fluorescent antibodies against cyclobutane-pyrimidin-dimers (CPDs) in combination with immuno-fluorescence image analysis we quantitatively compared fs-irradiation effects with UV-irradiation (solar simulator). Based on these results we are giving a risk assessment. The results show that multi photon imaging using the parameters described here is in the ballpark of damaging occurring from every day sun exposure.
Fischer
Frank
F
Beiersdorf AG, Unnastrasse 48, 20245 Hamburg, Germany. frank.fischer@beiersdorf.com
Volkmer
Beate
B
Puschmann
Stefan
S
Greinert
Rüdiger
R
Breitbart
Eckhard
E
Kiefer
Jürgen
J
Wepf
Roger
R