Conjugation with alpha-linolenic acid improves cancer cell uptake and cytotoxicity of doxorubicin.
The synthetic DOX-LNA conjugate was characterized by proton nuclear magnetic resonance and mass spectrometry. In addition, the purity of the conjugate was analyzed by reverse-phase high-performance liquid chromatography. The cellular uptake, intracellular distribution, and cytotoxicity of DOX-LNA were assessed by flow cytometry, fluorescence microscopy, liquid chromatography/electrospray ionization tandem mass spectrometry, and the tetrazolium dye assay using the in vitro cell models. The DOX-LNA conjugate showed substantially higher tumor-specific cytotoxicity compared with DOX.
Huan
Meng-Lei
ML
Department of Pharmacology, School of Pharmacy, Fourth Military Medical University, 17, Changle West Road, Xi'an, Shaan'xi 710032, China.
Zhou
Si-Yuan
SY
Teng
Zeng-Hui
ZH
Zhang
Bang-le
BL
Liu
Xin-You
XY
Wang
Jie-Pin
JP
Mei
Qi-Bing
QB