Oxidation of melatonin by AAPH-derived peroxyl radicals: evidence of a pro-oxidant effect of melatonin.
BACKGROUND
Melatonin is well-established as a powerful reducing agent of oxidant generated in the cell medium. We aimed to investigate how readily melatonin is oxidized by peroxyl radicals ROO generated by the thermolysis of 2,2'-azobis(2-amidinopropane) hydrochloride (AAPH) and the role of glutathione (GSH) during the reaction course.


METHODS
Chromatographic, mass spectroscopy, and UV-visible spectrometric techniques were used to study the oxidation of melatonin by ROO or horseradish peroxidase (HRP)/H2O2. Our focus was the characterization of products and the study of features of the reaction.


RESULTS
We found that N(1)-acetyl-N(2)-formyl-5-methoxykynuramine (AFMK) and a monohydroxylated derivative of melatonin were the main products of the reaction between melatonin and ROO. Higher pH or saturation of the medium with molecular oxygen increased the yield of AFMK but did not affect the reaction rate. Melatonin increased the depletion of intracellular GSH mediated by AAPH. Using the HRP/H2O2 as the oxidant system, the addition of melatonin promoted the oxidation of GSH to GSSG.


CONCLUSIONS
These results show, for the first time, that melatonin radical is able to oxidize GSH.


CONCLUSIONS
We propose that this new property of melatonin could explain or be related to the recently reported pro-oxidant activities of melatonin.
Ximenes
Valdecir F
VF
Departamento de Química, Faculdade de Ciências, Universidade Estadual Paulista "Júlio de Mesquita Filho", Bauru, SP, Brazil. vfximenes@fc.unesp.br
Pessoa
Adriano S
AS
Padovan
Camila Z
CZ
Abrantes
Daniele C
DC
Gomes
Fabiana Helena F
FH
Maticoli
Michele A
MA
de Menezes
Manoel L
ML