Analysis of cyclosporin A in dried blood spots using liquid chromatography tandem mass spectrometry.
Dried blood spot sampling is a promising and patient friendly alternative for venous sampling. A liquid chromatography tandem mass spectrometer assay was developed for analyzing cyclosporin A in dried blood spots. Linearity ranged from 25 to 1440 microg/L. Within and between run accuracy and precision were within limits. The developed assay has a negligible matrix-effect and a recovery of 97%. The dried blood spots were stable during a period of at least 17 days in the refrigerator. The developed assay is suitable for analyzing cyclosporin A in dried blood spots.
Wilhelm
A J
AJ
VU University Medical Center, Laboratory of Pharmacology and Pharmacy, HV, Amsterdam, Netherlands.
den Burger
J C G
JC
Vos
R M
RM
Chahbouni
A
A
Sinjewel
A
A