Relative orientation of rigid nitroxides by PELDOR: beyond distance measurements in nucleic acids.
Show me your angle: Incorporation of the rigid spin label C allows determination of both distance and orientation of two nitroxide spin labels in DNA by PELDOR experiments at common X-band frequencies. The orientational information is obtained by varying the position of the detection pulses over the nitroxide spectrum. Simulation of the set of time traces yields very precise distances and angles.
Schiemann
Olav
O
Centre for Biomolecular Sciences, University of St. Andrews, North Haugh, St Andrews KY 1619ST, UK. os11@st-andrews.ac.uk
Cekan
Pavol
P
Margraf
Dominik
D
Prisner
Thomas F
TF
Sigurdsson
Snorri Th
ST