title: One-step PCR for the identification of multiple antimicrobial resistance in Neisseria gonorrhoeae.
abstract: One-step multiplex PCR was developed for the identification of gonococci and antimicrobial-resistant profiles. From forty Neisseria gonorrhoeae isolates, the penicillinase-producing N. gonorrhoeae (PPNG), the high-level tetracycline-resistant N. gonorrhoeae (TRNG), and the ciprofloxacin-resistant N. gonorrhoeae (CRNG) were successfully classified. Our method provides expediency and benefit to epidemiology and antimicrobial-resistance mobility with 100% sensitivity and specificity for gonococcal-detection. The detection limit was 500 CFU/reaction.
authors.affiliation: Department of Clinical Microbiology, Faculty of Medical Technology, Mahidol University, Bangkok 10700, Thailand.
authors.forename: Ratana
authors.initials: R
authors.lastname: Lawung
authors.forename: Rungrot
authors.initials: R
authors.lastname: Cherdtrakulkiat
authors.forename: Angkana
authors.initials: A
authors.lastname: Charoenwatanachokchai
authors.forename: Sunanta
authors.initials: S
authors.lastname: Nabu
authors.forename: Wanvisa
authors.initials: W
authors.lastname: Suksaluk
authors.forename: Virapong
authors.initials: V
authors.lastname: Prachayasittikul