title: The influence of reactive oxygen species on the adhesion of pancreatic carcinoma cells to the peritoneum.
abstract: Postoperative peritoneal carcinomatosis is a significant clinical problem after "curative" resection of pancreatic carcinoma. Preoperative surgical trauma activates a cascade of peritoneal defense mechanisms responsible for postoperative intra-abdominal tumor recurrence. Reactive oxygen species (ROS) play a pivotal role in this postoperative inflammatory reaction. This study explores the influence of ROS on adhesion of human pancreatic carcinoma cells to human mesothelial cells. Furthermore this study explores the influence of ROS on the presentation of adhesion molecules on Panc-1 and mesothelial cells. ROS were produced using the enzymatic reaction of xanthine with xanthine oxidase (X/XO). A reproducible in vitro assay to study adhesion of human Panc-1 carcinoma tumor cells to a mesothelial cell monolayer of primary human mesothelial cells was used. Mesothelial monolayers were incubated with ROS produced prior to adhesion of the tumor cells. Incubation of the mesothelial cells with X/XO resulted in a significant increase (69.5%) in adhesion of Panc-1 in all patients. SOD/catalase, anti-oxidants, could reduce this increase by 56.7%. ROS significantly influenced the expression of the adhesion molecules ICAM-1, VCAM-1 and CD44h on mesothelial cells, but did not influence adhesion molecule expression on Panc-1. The ROS released during the post-operative inflammatory reaction may play an important role in the adhesion of pancreatic tumor cells to the mesothelium-possibly by influencing adhesion molecule expression on mesothelial cells. Therefore ROS can partly be responsible for the enhanced post-operative intra-abdominal tumor recurrence.
authors.affiliation: Department of Surgery, Erasmus MC, Rotterdam, The Netherlands. sandertenraa@ hotmail.com
authors.forename: Sander
authors.initials: S
authors.lastname: ten Raa
authors.forename: Helma M U
authors.initials: HM
authors.lastname: van Grevenstein
authors.forename: Miranda
authors.initials: M
authors.lastname: ten Kate
authors.forename: Kristin M
authors.initials: KM
authors.lastname: Mangundap
authors.forename: Leo J
authors.initials: LJ
authors.lastname: Hofland
authors.forename: Hans
authors.initials: H
authors.lastname: Jeekel
authors.forename: Wim
authors.initials: W
authors.lastname: Sluiter
authors.forename: Casper H J
authors.initials: CH
authors.lastname: van Eijck