Mapping organelle proteins and protein complexes in Drosophila melanogaster.
Many proteins within eukaryotic cells are organized spatially and functionally into membrane bound organelles and complexes. A protein's location thus provides information about its function. Here, we apply LOPIT, a mass-spectrometry based technique that simultaneously maps proteins to specific subcellular compartments, to Drosophila embryos. We determine the subcellular distribution of hundreds of proteins, and protein complexes. Our results reveal the potential of LOPIT to provide average snapshots of cells.
Tan
Denise J L
DJ
Cambridge Systems Biology Centre, Department of Biochemistry, Tennis Court Road, Cambridge CB2 1QR, United Kingdom.
Dvinge
Heidi
H
Christoforou
Andrew
A
Bertone
Paul
P
Martinez Arias
Alfonso
A
Lilley
Kathryn S
KS