title: Expandase-like activity mediated cell-free conversion of ampicillin to cephalexin by Streptomyces sp. DRS I.
abstract: Cell-free extracts of Streptomyces sp. DRS I converted ampicillin to cephalexin, presumably due to the activity of the enzyme, expandase. The extract was fractionated and characterized by colorimetric and chromatographic measurements coupled with disc-agar diffusion bioassay against an ampicillin-resistant, cephalexin-sensitive E. coli strain. Though expandase could not be identified, the presence of a hitherto unreported expandase in Streptomyces sp. DRS I is suggested.
authors.affiliation: Biotechnology Division, North East Institute of Science and Technology (CSIR), Jorhat, 785006, Assam, India. debajitthakur@fastmail.fm
authors.forename: D
authors.initials: D
authors.lastname: Thakur
authors.forename: M K
authors.initials: MK
authors.lastname: Roy
authors.forename: T C
authors.initials: TC
authors.lastname: Bora