title: Particle tracking microrheology of gel-forming amyloid fibril networks.
abstract: Microrheology is a technique that is increasingly used to investigate the local viscoelastic properties of complex fluids non-invasively, by tracking the motion of micron-sized probe spheres. In this work, passive Particle Tracking Microrheology (PTM) is used to study network formation in the milk protein beta-lactoglobulin at 80 degrees C and pH 2. In these conditions the protein aggregates to form thread-like structures known as amyloid fibrils, which can further aggregate into elastic networks. Using PTM, gels were observed to form at significantly lower concentrations than determined by bulk rheometry, where the oscillatory shear forces may disrupt either fibril or network formation. During incubation, the Mean Square Displacement (MSD) of the probe particles exhibited time-cure superposition, allowing the critical relaxation exponent to be calculated as approximately 0.63, consistent with other biopolymer gels. Combined with the gel-like appearance of the complex modulus at long incubation times, this confirms that a true gel is forming, with physical or chemical crosslinks forming between the fibrils, refining the conclusions of other workers in the field.
authors.affiliation: Sector of Biological & Soft Systems, Department of Physics, University of Cambridge, Cavendish Laboratory, J. J. Thomson Avenue, Cambridge CB3 0HE, UK.
authors.forename: A M
authors.initials: AM
authors.lastname: Corrigan
authors.forename: A M
authors.initials: AM
authors.lastname: Donald