title: Sphingomonas sanxanigenens sp. nov., isolated from soil.
abstract: Strain NX02(T), a Gram-negative, non-spore-forming, rod-shaped bacterium, was isolated from soil, and its taxonomic position was investigated using a polyphasic approach. Chemotaxonomic analysis revealed that strain NX02(T) possessed Q-10 as the predominant ubiquinone, sym-homospermidine as the major polyamine and C(18 : 1)omega7c, C(16 : 0) and C(14 : 0) 2-OH as the major fatty acids. The main polar lipids were sphingoglycolipid, phosphatidylcholine, phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylmonomethylethanolamine, phosphatidyldimethylethanolamine and an unidentified glycolipid. The DNA G+C content was 66.4 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain NX02(T) belongs to the alpha-4 subgroup of the Proteobacteria, exhibiting the highest sequence similarity with respect to Sphingomonas azotifigens NBRC 15497(T) (95.9 %), Sphingomonas pituitosa DSM 13101(T) (95.8 %) and Sphingomonas dokdonensis KCTC 12541(T) (95.8 %). On the basis of these results, strain NX02(T) represents a novel species of the genus Sphingomonas sensu stricto, for which the name Sphingomonas sanxanigenens sp. nov. is proposed. The type strain is NX02(T) (=DSM 19645(T) =CGMCC 1.6417(T)).
authors.affiliation: Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, College of Life Sciences, Nankai University, Tianjin 300071, PR China.
authors.forename: Hai-Dong
authors.initials: HD
authors.lastname: Huang
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.forename: Ting
authors.initials: T
authors.lastname: Ma
authors.forename: Guo-Qiang
authors.initials: GQ
authors.lastname: Li
authors.forename: Feng-Lai
authors.initials: FL
authors.lastname: Liang
authors.forename: Ru-Lin
authors.initials: RL
authors.lastname: Liu