title: Potent antitumor effect of interleukin-24 gene in the survivin promoter and retinoblastoma double-regulated oncolytic adenovirus.
abstract: Interleukin (IL)-24 is an excellent therapeutic gene for cancer therapy. In this work, IL-24 was inserted into Ad.sp-E1A(Delta24), an oncolytic adenovirus with a 24-bp deletion in the E1A gene, which was driven by the survivin promoter to form Ad.sp-E1A(Delta24)-IL-24. Ad.sp-E1A(Delta24)-IL-24 has an excellent antitumor effect in vitro for human nasopharyngeal, liver, lung, and cervical carcinoma cell lines but does no or little damage to normal cell lines L-02 and WI38. Furthermore, it achieved nearly complete inhibition (although not elimination) of NCI-H460 lung carcinoma growth in nude mice. The antitumor efficacy of Ad.sp-E1A(Delta24)-IL-24 on NCI-H460 cells was clearly mediated by apoptosis, because it induced caspase-3 and poly(ADP-ribose) polymerase cleavage. This is the first report of Ad.sp-E1A(Delta24)-IL-24 with such an excellent, broad, and specific antitumor effect in vitro and nearly complete inhibition of lung tumor growth in vivo.
authors.affiliation: School of Life Sciences, Xinyuan Institute of Medicine and Biotechnology, Zhejiang Sci-Tech University, Hangzhou 310018, China.
authors.forename: Kang Jian
authors.initials: KJ
authors.lastname: Zhang
authors.forename: Yi Gang
authors.initials: YG
authors.lastname: Wang
authors.forename: Xin
authors.initials: X
authors.lastname: Cao
authors.forename: Su Yang
authors.initials: SY
authors.lastname: Zhong
authors.forename: Rui Cheng
authors.initials: RC
authors.lastname: Wei
authors.forename: Yu Mei
authors.initials: YM
authors.lastname: Wu
authors.forename: Xue Tian
authors.initials: XT
authors.lastname: Yue
authors.forename: Gong Chu
authors.initials: GC
authors.lastname: Li
authors.forename: Xin Yuan
authors.initials: XY
authors.lastname: Liu