title: Prevention of peritoneal carcinomatosis in mice with combination hyperthermal intraperitoneal chemotherapy and IL-2.
abstract: OBJECTIVE
  The purpose of this study was to investigate the effect of local chemoimmunotherapy and hyperthermal intraperitoneal chemotherapy (HIPEC) in a mouse model of induced peritoneal carcinomatosis.
  
  
  METHODS
  Peritoneal carcinomatosis in mice was produced by intraperitoneal implantation of MCa cells (5 x 10(3)). Interleukin-2 (4.1 x 10(4) IU/mouse) was injected into the abdominal cavity of mice at day 7 and 3 before implantation of tumour cells. Immediately after implantation of MCa cells mice were treated twice with 2 ml of saline that was heated either at 37 degrees C or 43 degrees C and cytostatics (doxorubicin 20 mg kg(-1), cisplatin 10 mg kg(-1), mitomycin 5 mg kg(-1), or 5-FU 150 mg kg(-1)). We followed the survival of animals and side effects appearing with different forms of treatment.
  
  
  RESULTS
  Combined treatment with Interleukin-2 (IL-2) and cytostatics (5-FU, CIS or MIT) significantly affected the development of peritoneal carcinomatosis and increased the survival of mice (ILS% - 37 degrees C = 29.88, 199.32, and 108.52, ILS% - 43 degrees C = 62.69, 260.50, and 178.05, respectively). However, intraperitoneal chemotherapy on survival time of mice with DOX + IL-2 was ineffective as compared with DOX alone.
  
  
  CONCLUSIONS
  We would like to stress that treatment with IL-2 prior to tumour diagnosis is not clinically practical, rather, the manuscript attempts to describe an experimental proof of principle. Results suggest the synergistic effect of hyperthermia, chemotherapy and immunotherapy; IL-2 significantly increases antitumor activity of hyperthermic chemotherapy and survival rate of mice with peritoneal carcinomatosis.
authors.affiliation: Department of Internal Medicine, Clinical Hospital Mostar, Mostar, Bosnia and Herzegovina, Croatia.
authors.forename: Milenko
authors.initials: M
authors.lastname: Bevanda
authors.forename: Nada
authors.initials: N
authors.lastname: Orsolic
authors.forename: Ivan
authors.initials: I
authors.lastname: Basic
authors.forename: Katarina
authors.initials: K
authors.lastname: Vukojevic
authors.forename: Vesna
authors.initials: V
authors.lastname: Benkovic
authors.forename: Anica
authors.initials: A
authors.lastname: Horvat Knezevic
authors.forename: Duje
authors.initials: D
authors.lastname: Lisicic
authors.forename: Domagoj
authors.initials: D
authors.lastname: Dikic
authors.forename: Milan
authors.initials: M
authors.lastname: Kujundzic