Effects of hCG and ovarian steroid hormones on vasotocin levels in the female catfish Heteropneustes fossilis.
Effects of hCG, ovariectomy and estradiol replacement on brain, plasma and/or ovarian vasotocin in vivo, and estradiol, progesterone, 17alpha, 20beta-hydroxy-4-pregnen-3-one and hCG on ovarian vasotocin in vitro were investigated in the catfish. A 100IU/fish of hCG induced ovulation and elicited both periovulatory and post-ovulatory changes in vasotocin concentrations with a significant increase up to 8h in the brain and up to 16h in both plasma and ovary. After stripping the fish at 16h, the peptide concentration decreased significantly with time, up to 4 days. Ovariectomy in early pre-spawning phase resulted in a duration-dependent significant reduction of both brain and plasma vasotocin. Estradiol replacement in 3-week ovariectomized fish produced dosage-dependent biphasic effects: the lower dosage (0.1microg/g) restored the vasotocin level while the higher dosage (0.5microg/g) decreased it significantly below the control level. In vitro incubation of ovarian tissues with estradiol produced season-dependent effects on vasotocin. The incubation of pre-vitellogenic ovarian pieces with estradiol (1, 10, and 100ng/ml) elevated vasotocin level in a dose- and duration-dependent manner while that of post-vitellogenic follicles resulted in a significant decrease. The incubation of intact post-vitellogenic follicles or follicular envelope with progesterone and 17alpha, 20beta-hydroxy-4-pregnen-3-one (1microg/ml) or hCG (20IU/ml) for 8 and 16h significantly increased vasotocin in a duration-dependent manner. The results show that both gonadotropin and ovarian steroids modulate vasotocin titer, which may influence follicular growth, ovulation and spawning in the catfish.
Singh
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Center of Advanced Study, Department of Zoology, Banaras Hindu University, Uttar Pradesh, India.
Joy
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KP