title: Important role of heparan sulfate in postnatal islet growth and insulin secretion.
abstract: Heparan sulfate (HS) binds with several signaling molecules and regulates ligand-receptor interactions, playing an essential role in embryonic development. Here we showed that HS was intensively expressed in pancreatic islet beta-cells after 1 week of age in mice. The enzymatic removal of HS in isolated islets resulted in attenuated glucose-induced insulin secretion with a concomitant reduction in gene expression of several key components in the insulin secretion machinery. We further depleted islet HS by inactivating the exostosin tumor-like 3 gene specifically in beta-cells. These mice exhibited abnormal islet morphology with reduced beta-cell proliferation after 1 week of age and glucose intolerance due to defective insulin secretion. These results demonstrate that islet HS is involved in the regulation of postnatal islet maturation and required to ensure normal insulin secretion.
authors.affiliation: Department of Advanced Biological Sciences for Regeneration, Tohoku University Graduate School of Medicine, Sendai, Japan.
authors.forename: Iwao
authors.initials: I
authors.lastname: Takahashi
authors.forename: Naoya
authors.initials: N
authors.lastname: Noguchi
authors.forename: Koji
authors.initials: K
authors.lastname: Nata
authors.forename: Shuhei
authors.initials: S
authors.lastname: Yamada
authors.forename: Tomoyuki
authors.initials: T
authors.lastname: Kaneiwa
authors.forename: Shuji
authors.initials: S
authors.lastname: Mizumoto
authors.forename: Takayuki
authors.initials: T
authors.lastname: Ikeda
authors.forename: Kazushi
authors.initials: K
authors.lastname: Sugihara
authors.forename: Masahide
authors.initials: M
authors.lastname: Asano
authors.forename: Takeo
authors.initials: T
authors.lastname: Yoshikawa
authors.forename: Akiyo
authors.initials: A
authors.lastname: Yamauchi
authors.forename: Nausheen Jamal
authors.initials: NJ
authors.lastname: Shervani
authors.forename: Akira
authors.initials: A
authors.lastname: Uruno
authors.forename: Ichiro
authors.initials: I
authors.lastname: Kato
authors.forename: Michiaki
authors.initials: M
authors.lastname: Unno
authors.forename: Kazuyuki
authors.initials: K
authors.lastname: Sugahara
authors.forename: Shin
authors.initials: S
authors.lastname: Takasawa
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Okamoto
authors.forename: Akira
authors.initials: A
authors.lastname: Sugawara