title: Cytopathology and exocrine dysfunction induced in ex vivo rabbit lacrimal gland acinar cell models by chronic exposure to histamine or serotonin.
abstract: OBJECTIVE
  Lacrimal immunohistopathology has diverse clinical presentations, suggesting that inflammatory mediators exert diverse influences. Chronic exposure to agonistic acetylcholine receptor autoantibodies has been studied previously; the present work addressed mediators that signal through other G protein-coupled receptors.
  
  
  METHODS
  Acinus-like structures and reconstituted acinar epithelial monolayers from rabbit lacrimal glands were exposed to varying concentrations of histamine or 5-hydroxytryptamine (5-HT) for 20 hours. Net and vectorial beta-hexosaminidase secretion, cytosolic Ca(2+) (Ca(i)) elevation, apical recruitment of p150(Glued), actin microfilament meshwork organization, and ultrastructure were assessed.
  
  
  RESULTS
  Histamine and 5-HT acutely stimulated beta-hexosaminidase secretion at lower, but not higher, concentrations. Neither of them acutely elevated Ca(i) levels. Both recruited p150(Glued) at concentrations that failed to induce secretion. Chronic exposure to 10 mM histamine inhibited carbachol (CCh)-induced beta-hexosaminidase secretion and prevented the formation of continuous monolayers; 1 mM 5-HT partially inhibited secretion at the apical medium. Neither altered secretion to the basal medium. Chronic exposure to histamine or 5-HT partially decreased CCh induced Ca(i) elevations and p150(Glued) recruitment, even at concentrations that did not inhibit secretion. Both expanded acinar lumina and thickened microfilament meshworks, and both caused homotypic fusion of secretory vesicles and formation of aqueous vacuoles in the apical and basal cytoplasm. Chronic exposure to forskolin, which activates adenylyl cyclase, induced similar cytopathologic changes but impaired secretion modestly and only at the highest concentration tested.
  
  
  CONCLUSIONS
  Inflammatory mediators that signal through G protein-coupled receptors cause acinar cell cytopathology and dose-dependent reductions of CCh-induced beta-hexosaminidase secretion. Although agonistic acetylcholine receptor autoantibodies may cause pervasive functional quiescence, inflammatory mediators may cause varying degrees of exocrine dysfunction.
authors.affiliation: Department of Physiology and Biophysics, Keck School of Medicine, Los Angeles, California 90089-9142, USA.
authors.forename: Michelle L
authors.initials: ML
authors.lastname: McDonald
authors.forename: Yanru
authors.initials: Y
authors.lastname: Wang
authors.forename: Shivaram
authors.initials: S
authors.lastname: Selvam
authors.forename: Tamako
authors.initials: T
authors.lastname: Nakamura
authors.forename: Robert H
authors.initials: RH
authors.lastname: Chow
authors.forename: Joel E
authors.initials: JE
authors.lastname: Schechter
authors.forename: Samuel C
authors.initials: SC
authors.lastname: Yiu
authors.forename: Austin K
authors.initials: AK
authors.lastname: Mircheff