[Screening of serum protein biomarkers by surface-enhanced laser desorption and ionization with time-of-flight detection mass spectrometry in patients with mycosis fungoides].
OBJECTIVE
To screen serum biomarkers in patients with mycosis fungoides (MF) using surface-enhanced laser desorption and ionization with time-of-flight detection mass spectrometry (SELDI-TOF-MS) technique.


METHODS
Serum was analyzed from 14 patients with MF and 17 controls using CM10 Protein-chip to capture serum proteins, followed by Biomarker Wizard software analysis.


RESULTS
In all specimens, about 131 protein peaks could be detected when the relative molecular weight ranged from 0 to 50 000. When comparing the protein fingerprint between these two groups, 14 differentially expressed protein peaks were found. By searching SWISS-PRO database, we found 7 670Da peaks accord with C-C motif chemokine 22.


CONCLUSIONS
SELDI-TOF-MS technique can be used for screening serum protein biomarkers in patients with MF.
Liu
Jie
J
Department of Dermatology, PUMC Hospital, CAMS and PUMC, Beijing 100730, China.
Zheng
Yan-Hua
YH
Zhang
Jian-Zhong
JZ
Wang
Bao-Xi
BX