High-throughput screening of catalytically inactive mutants of protein tyrosine phosphatases (PTPs) in a phosphopeptide microarray.
We report herein a novel phosphopeptide microarray capable of noncovalently "trapping" catalytically inactive mutants of protein tyrosine phosphatases (PTPs), and its application in high-throughput determination of PTP substrate specificity.
Sun
Hongyan
H
Department of Chemistry, National University of Singapore, 3 Science Drive, Singapore 11754.
Tan
Lay Pheng
LP
Gao
Liqian
L
Yao
Shao Q
SQ