title: Flexible low-cost system for small animal aerosol inhalation exposure to drugs, proteins, inflammatory agents, and infectious agents.
abstract: The design for a simple, low-cost aerosol generation system for rodent inhalation studies is described here. This system is appropriate for low biohazard-level agents. In this study, two biosafety level 2 agents, Pasturella pneumotropica and Pseudomonas aeruginosa, were tested successfully. This system was also used to immunize mice and guinea pigs in ovalbumin-based models of pulmonary inflammation. This design is appropriate for studies with limited budgets and lower-level biosafety containment.
authors.affiliation: Department of Microbiology, Immunology & Pathology, Colorado State University, Fort Collins, CO 80523, USA.
authors.forename: William G
authors.initials: WG
authors.lastname: Schroeder
authors.forename: Laura M
authors.initials: LM
authors.lastname: Mitrescu
authors.forename: Marcia L
authors.initials: ML
authors.lastname: Hart
authors.forename: Ranjana
authors.initials: R
authors.lastname: Unnithan
authors.forename: John M
authors.initials: JM
authors.lastname: Gilchrist
authors.forename: Erin E
authors.initials: EE
authors.lastname: Smith
authors.forename: Crystal
authors.initials: C
authors.lastname: Shanley
authors.forename: Katharine M
authors.initials: KM
authors.lastname: Benedict
authors.forename: Lauren
authors.initials: L
authors.lastname: Taraba
authors.forename: John
authors.initials: J
authors.lastname: Volckens
authors.forename: Randall J
authors.initials: RJ
authors.lastname: Basaraba
authors.forename: Alan R
authors.initials: AR
authors.lastname: Schenkel