title: Pathogenesis of steroid-resistant airway hyperresponsiveness: interaction between IFN-gamma and TLR4/MyD88 pathways.
abstract: Chronic inflammation and airway hyperresponsiveness (AHR) in asthmatics are usually managed effectively by treatment with glucocorticoids. However, a subset of patients remains refractory to therapy. The underlying mechanisms are largely unknown, although recruitment of neutrophils (rather than eosinophils) is strongly correlated, suggesting a role for nonallergic host defense responses. Increased levels of IFN-gamma and endotoxins are also related to severe asthma and because these activate host defense pathways, we investigated a possible common etiologic link to steroid-resistant disease. To be able to unravel the complexity of asthmatic inflammation, we used two model systems which permitted dissection of the relevant molecular pathways. In the first of these, we transferred transgenic OVA(323-339) peptide-specific IFN-gamma-producing Th1 cells into mice. These animals were subsequently challenge via the airways with OVA(323-339) peptide and/or LPS. Challenge with both components, but not with either one individually, induced AHR. Importantly, AHR was resistant to treatment with dexamethasone. Development of AHR was dependent on IFN-gamma, inhibited by depletion of pulmonary macrophages (but not neutrophils) and abrogated in TLR4- or MyD88-deficient mice. In contrast, in the second model in which we transferred OVA(323-339) peptide-activated Th2 cells, eosinophilic inflammation and AHR were induced, and both were suppressed by steroid treatment. We conclude that cooperative signaling between IFN-gamma and TLR4/MyD88 constitutes a previously unrecognized pathway that regulates macrophage-dependent steroid-resistant AHR.
authors.affiliation: Centre for Asthma and Respiratory Disease, School of Biomedical Sciences, University of Newcastle and Hunter Medical Research Institute, Callaghan, Australia.
authors.forename: Ming
authors.initials: M
authors.lastname: Yang
authors.forename: Rakesh K
authors.initials: RK
authors.lastname: Kumar
authors.forename: Paul S
authors.initials: PS
authors.lastname: Foster