Overexpression, purification and crystallization of a thermostable DNA ligase from the archaeon Thermococcus sp. 1519.
DNA ligases catalyze the sealing of 5'-phosphate and 3'-hydroxyl termini at single-strand breaks in double-stranded DNA and their function is essential to maintain the integrity of the genome in DNA metabolism. An ATP-dependent DNA ligase from the archaeon Thermococcus sp. 1519 was overexpressed, purified and crystallized. Crystals were obtained using the hanging-drop vapour-diffusion method employing 35%(v/v) Tacsimate pH 7.0 as a precipitant and diffracted X-rays to 3.09 A resolution. They belonged to space group P4(1)2(1)2, with unit-cell parameters a = b = 79.7, c = 182.6 A.
Bezsudnova
E Y
EY
Bach Institute of Biochemistry RAS, Leninsky Prospect 33, 119071 Moscow, Russia. eubez@yandex.ru
Kovalchuk
M V
MV
Mardanov
A V
AV
Poliakov
K M
KM
Popov
V O
VO
Ravin
N V
NV
Skryabin
K G
KG
Smagin
V A
VA
Stekhanova
T N
TN
Tikhonova
T V
TV