title: Optical knock out of stem cells with extremely ultrashort femtosecond laser pulses.
abstract: Novel ultracompact multiphoton sub-20 femtosecond near infrared 85 MHz laser scanning microscopes and conventional 250 fs laser microscopes have been used to perform high spatial resolution two-photon imaging of stem cell clusters as well as selective intracellular nanoprocessing and knock out of living single stem cells within an 3D microenvironment without any collateral damage. Also lethal cell exposure of large parts of cell clusters was successfully probed while maintaining single cells of interest alive. The mean power could be kept in the milliwatt range for 3D nanoprocessing and even in the microwatt range for two-photon imaging. Ultracompact low power sub-20 fs laser systems may become interesting tools for optical nanobiotechnology such as optical cleaning of stem cell clusters as well as optical transfection.
authors.affiliation: Fraunhofer Institute for Biomedical Technology IBMT, Ensheimer Strasse 48, 66386 St. Ingbert, Germany.
authors.forename: Aisada
authors.initials: A
authors.lastname: Uchugonova
authors.forename: Andreas
authors.initials: A
authors.lastname: Isemann
authors.forename: Erwin
authors.initials: E
authors.lastname: Gorjup
authors.forename: Gabriel
authors.initials: G
authors.lastname: Tempea
authors.forename: Rainer
authors.initials: R
authors.lastname: Bückle
authors.forename: Wataru
authors.initials: W
authors.lastname: Watanabe
authors.forename: Karsten
authors.initials: K
authors.lastname: König