Expression, purification, crystallization and preliminary X-ray crystallographic analysis of the SH3 domain of human AHI1.
The SH3 domain of human AHI1 was cloned and expressed in Escherichia coli. The protein was purified by affinity and size-exclusion chromatography and was crystallized using the sitting-drop vapour-diffusion method at 293 K. A complete data set was collected to 2.5 A resolution at 110 K. The crystal belonged to space group P4(1)2(1)2, with unit-cell parameters a = 67.377, b = 67.377, c = 98.549 A.
Shi
Zhuliang
Z
Guangzhou Institute of Biomedicine and Health, Chinese Academy of Sciences, People's Republic of China.
Liang
Ning
N
Xu
Wei
W
Li
Kuai
K
Sheng
Guoqing
G
Liu
Jinsong
J
Xu
Aimin
A
Li
Xiao Jiang
XJ
Wu
Donghai
D