title: High speed unsupervised fluorescence lifetime imaging confocal multiwell plate reader for high content analysis.
abstract: We report an automated optically sectioning fluorescence lifetime imaging (FLIM) multiwell plate reader for high content analysis (HCA) in drug discovery and accelerated research in cell biology. The system utilizes a Nipkow disc confocal microscope and performs unsupervised FLIM with autofocus, automatic setting of acquisition parameters and automated localisation of cells in the field of view. We demonstrate its applications to test dye solutions, fixed and live cells and FLIM-FRET.
authors.affiliation: Photonics Group, Imperial College London, London SW72AZ, UK. clifford.talbot@imperial.ac.uk
authors.forename: Clifford B
authors.initials: CB
authors.lastname: Talbot
authors.forename: James
authors.initials: J
authors.lastname: McGinty
authors.forename: David M
authors.initials: DM
authors.lastname: Grant
authors.forename: Ewan J
authors.initials: EJ
authors.lastname: McGhee
authors.forename: Dylan M
authors.initials: DM
authors.lastname: Owen
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.forename: Tom D
authors.initials: TD
authors.lastname: Bunney
authors.forename: Ian
authors.initials: I
authors.lastname: Munro
authors.forename: Beverly
authors.initials: B
authors.lastname: Isherwood
authors.forename: Rob
authors.initials: R
authors.lastname: Eagle
authors.forename: Andre
authors.initials: A
authors.lastname: Hargreaves
authors.forename: Chris
authors.initials: C
authors.lastname: Dunsby
authors.forename: Mark A A
authors.initials: MA
authors.lastname: Neil
authors.forename: Paul M W
authors.initials: PM
authors.lastname: French