title: A longitudinal cohort study in calves evaluated for rotavirus infections from 1 to 12 months of age by sequential serological assays.
abstract: Using an immunocytochemical staining assay involving six different recombinant baculoviruses with each expressing one of the major bovine rotavirus VP7 (G6, G8 and G10) and VP4 (P6[1], P7[5] and P8[11]) serotypes, we analyzed IgG antibody responses to individual proteins in archival serum samples collected from 31 calves monthly from 1 to 12 months of age during 1974-1975 in Higley, Arizona. Seroresponses to VP7 and VP4, as determined by a fourfold or greater antibody response, were not always elicited concurrently following infection: in some calves, (1) seroresponses to VP7 were detected earlier than to VP4 or vice versa; and (2) a subsequent second seroresponse was detected for VP7 or VP4 only. In addition, a second infection was more likely to be caused by different G and/or P types. Analyses of serum samples showed that the most frequent G-P combination was G8P6[1], followed by G8P7[5], G8P8[11] and G6P6[1].
authors.affiliation: Epidemiology Section, Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Building 50, Room 6308, Bethesda, MD 20892, USA.
authors.forename: Dianjun
authors.initials: D
authors.lastname: Cao
authors.forename: Blessing
authors.initials: B
authors.lastname: Igboeli
authors.forename: Lijuan
authors.initials: L
authors.lastname: Yuan
authors.forename: Albert Z
authors.initials: AZ
authors.lastname: Kapikian
authors.forename: Jess L
authors.initials: JL
authors.lastname: Ayers
authors.forename: Francis R
authors.initials: FR
authors.lastname: Abinanti
authors.forename: Yasutaka
authors.initials: Y
authors.lastname: Hoshino