title: High-resolution X-ray tomography of the human inner ear: synchrotron radiation-based study of nerve fibre bundles, membranes and ganglion cells.
abstract: The combination of osmium tetroxide staining and high-resolution tomographic imaging using monochromatic X rays allows visualizing cellular structures of the human inner ear, that is, the organ of Corti, the stria vascularis and further soft tissues of the membranous labyrinth, in three-dimensional space with isotropic micrometre resolution. This approach permits to follow the course of nerve fibre bundles in a major part of the specimen and reveals the detailed three-dimensional arrangement of individual ganglion cells with distinct nuclei by means of X-ray tomography for the first time. The non-destructive neuron cell counting in a selected volume of 125 microm x 800 microm x 600 microm = 0.06 mm(3) gives rise to the estimate that 2000 ganglion cells are present along 1 mm organ of Corti.
authors.affiliation: Biomaterials Science Center, University of Basel, c/o University Hospital, Basel, Switzerland.
authors.forename: A
authors.initials: A
authors.lastname: Lareida
authors.forename: F
authors.initials: F
authors.lastname: Beckmann
authors.forename: A
authors.initials: A
authors.lastname: Schrott-Fischer
authors.forename: R
authors.initials: R
authors.lastname: Glueckert
authors.forename: W
authors.initials: W
authors.lastname: Freysinger
authors.forename: B
authors.initials: B
authors.lastname: Müller