Efficient bioethanol production by a recombinant flocculent Saccharomyces cerevisiae strain with a genome-integrated NADP+-dependent xylitol dehydrogenase gene.
The recombinant industrial Saccharomyces cerevisiae strain MA-R5 was engineered to express NADP(+)-dependent xylitol dehydrogenase using the flocculent yeast strain IR-2, which has high xylulose-fermenting ability, and both xylose consumption and ethanol production remarkably increased. Furthermore, the MA-R5 strain produced the highest ethanol yield (0.48 g/g) from nonsulfuric acid hydrolysate of wood chips.
Matsushika
Akinori
A
Biomass Technology Research Center, National Institute of Advanced Industrial Science and Technology, Kure, Hiroshima, Japan. a-matsushika@aist.go.jp
Inoue
Hiroyuki
H
Watanabe
Seiya
S
Kodaki
Tsutomu
T
Makino
Keisuke
K
Sawayama
Shigeki
S