Treatment with green tea extract attenuates secondary inflammatory response in an experimental model of spinal cord trauma.
In this study, we evaluated the effect of green tea extract (that was administered 25 mg/kg intraperitoneal at 1 and 6 h after injury) in experimental animal model of spinal cord injury. The spinal cord trauma was induced by the application of vascular clips to the dura via a four-level T5-T8 laminectomy. Spinal cord injury in mice resulted in severe trauma characterised by oedema, neutrophilic infiltration and apoptosis. Also, immunohistochemical examination demonstrated a marked increase in immune reactivity for nitrotyrosine. All parameters of inflammation were attenuated by green tea extract. The degree of spinal cord inflammation, nitrotyrosine, poli (ADP-ribosio) synthetase (PARS) and neutrophilic infiltration was markedly reduced. Green tea extract significantly ameliorated the recovery of limb function. Values shown are mean +/- SE mean of ten mice for each group. *p < 0.01 versus sham, degrees p < 0.01 versus spinal cord injury. Taken together, our results clearly demonstrate that green tea extract treatment ameliorates spinal cord injury oxidative stress.
Paterniti
Irene
I
Department of Clinical and Experimental Medicine and Pharmacology, School of Medicine, University of Messina, Torre Biologica, Gazzi, Messina, Italy.
Genovese
Tiziana
T
Crisafulli
Concetta
C
Mazzon
Emanuela
E
Di Paola
Rosanna
R
Galuppo
Maria
M
Bramanti
Placido
P
Cuzzocrea
Salvatore
S