title: [Use of multiplex PCR method with real-time detection for differential diagnosis of respiratory viral infections].
abstract: Multiplex real-time polymerase chain reaction test-system with fluorescent detection (RT-PCR) for simultaneous identification of main agents of acute respiratory viral infections: influenza A (IAV) and B viruses (IBV), parainfluenza viruses types 1, 2, 3, 4 (PIV 1 - 4), adenoviruses (ADV), respiratory syncitial virus (RSV), rhinoviruses (RV) and enteroviruses (EV), in presence internal positive control (IPC) represented by vaccine strain of rubella virus RA 27/3. Using multiplex RT-PCR method, respiratory viruses were detected in 116 out of 226 clinical samples (nasal swabs) obtained from patients with symptoms of acute respiratory infection: in 68 (58.6%) samples--IBV; in 21 (18.1%)--IAV; in 12 (10.3%) --RV; in 6 (5.2%)--PIV 2; in 4--(3.4%) ADV; in 3 (2.6%)--RSV; in 2 (1.7%)--EV; in 2 (1.7%)--PIV 4; in 1 (0.9%)--PIV 3; in 1 (0.9%)--PIV 1. Mixed infection was observed in 4 (3.4%) patients. PCR assay allowed to reveal various respiratory viruses in 51.3% of samples. At the same time samples were tested for the presence of 12 respiratory viruses--IAV, IBV, PIV 1 - 4, RSV, RV, metapneumoviruses, and coronaviruses NL63, 229E and OC43--in the presence of IPC represented by equine arteritis virus using analogous PCR test-system provided by medical center of Leiden university. Results of tests for detection of IAV, IBV, RSV, PIV 1 - 4, and RV, analyzed by both systems, agreed in 94%. Multiplex format of RT-PCR performing significantly reduces time and cost of the test, which make it suitable and effective instrument of epidemiological studies.
authors.forename: A A
authors.initials: AA
authors.lastname: Nikonova
authors.forename: E S
authors.initials: ES
authors.lastname: Uspenskaia
authors.forename: S A
authors.initials: SA
authors.lastname: Lobodanov
authors.forename: A S
authors.initials: AS
authors.lastname: Oksanich
authors.forename: A E
authors.initials: AE
authors.lastname: Gorbalenia
authors.forename: Eric C J
authors.initials: EC
authors.lastname: Claas
authors.forename: E P
authors.initials: EP
authors.lastname: Foshina
authors.forename: A N
authors.initials: AN
authors.lastname: Kaira
authors.forename: V V
authors.initials: VV
authors.lastname: Zverev
authors.forename: E B
authors.initials: EB
authors.lastname: Faĭzuloev