title: A robust linkage map of the porcine autosomes based on gene-associated SNPs.
abstract: BACKGROUND
  Genetic linkage maps are necessary for mapping of mendelian traits and quantitative trait loci (QTLs). To identify the actual genes, which control these traits, a map based on gene-associated single nucleotide polymorphism (SNP) markers is highly valuable. In this study, the SNPs were genotyped in a large family material comprising more than 5,000 piglets derived from 12 Duroc boars crossed with 236 Danish Landrace/Danish Large White sows. The SNPs were identified in sequence alignments of 4,600 different amplicons obtained from the 12 boars and containing coding regions of genes derived from expressed sequence tags (ESTs) and genomic shotgun sequences.
  
  
  RESULTS
  Linkage maps of all 18 porcine autosomes were constructed based on 456 gene-associated and six porcine EST-based SNPs. The total length of the averaged-sex whole porcine autosome was estimated to 1,711.8 cM resulting in an average SNP spacing of 3.94 cM. The female and male maps were estimated to 2,336.1 and 1,441.5 cM, respectively. The gene order was validated through comparisons to the cytogenetic and/or physical location of 203 genes, linkage to evenly spaced microsatellite markers as well as previously reported conserved synteny. A total of 330 previously unmapped genes and ESTs were mapped to the porcine autosome while ten genes were mapped to unexpected locations.
  
  
  CONCLUSIONS
  The linkage map presented here shows high accuracy in gene order. The pedigree family network as well as the large amount of meiotic events provide good reliability and make this map suitable for QTL and association studies. In addition, the linkage to the RH-map of microsatellites makes it suitable for comparison to other QTL studies.
authors.affiliation: Department of Genetics and Biotechnology, Faculty of Agricultural Sciences, Aarhus University, Tjele, Denmark. rikkek.vingborg@agrsci.dk
authors.forename: Rikke K K
authors.initials: RK
authors.lastname: Vingborg
authors.forename: Vivi R
authors.initials: VR
authors.lastname: Gregersen
authors.forename: Bujie
authors.initials: B
authors.lastname: Zhan
authors.forename: Frank
authors.initials: F
authors.lastname: Panitz
authors.forename: Anette
authors.initials: A
authors.lastname: Høj
authors.forename: Kirsten K
authors.initials: KK
authors.lastname: Sørensen
authors.forename: Lone B
authors.initials: LB
authors.lastname: Madsen
authors.forename: Knud
authors.initials: K
authors.lastname: Larsen
authors.forename: Henrik
authors.initials: H
authors.lastname: Hornshøj
authors.forename: Xuefei
authors.initials: X
authors.lastname: Wang
authors.forename: Christian
authors.initials: C
authors.lastname: Bendixen