title: Regulation of fibronectin matrix assembly and capillary morphogenesis in endothelial cells by Rho family GTPases.
abstract: Fibronectin (FN) fibrillogenesis is an essential biological process mediated by alpha5beta1 integrin and cellular contractile forces. Assembly of a FN matrix by activated endothelial cells occurs during angiogenic blood vessel remodeling and signaling components that control this event represent attractive therapeutic targets. Here we examined the role of individual Rho GTPases in FN matrix remodeling by selectively attenuating their expression in cultured endothelial cells. Whereas pharmacological ablation of myosin-regulated contractility abrogated matrix assembly, no significant decrease was detected in the amount of FN deposited by RhoA, RhoB-, RhoC-, Rac1-, or Cdc42-depleted cells. Rather, distinct differences in fiber arrangement were observed. Most strikingly, RhoA silenced cells assembled a fine FN meshwork beneath alpha5beta1 integrin-based fibrillar adhesions, in the absence of classical focal adhesions and actin stress fibers, indicating that alpha5beta1 integrin translocation and FN fibril elongation can occur in low tension states such as those encountered by newly-forming vessels in tissue. In contrast, highly contractile Cdc42-deficient cells deposited FN globules and Rac-deficient cells assembled long arrays, reflecting their increased motility. We propose that regulation of FN scaffolds by Rho GTPase signaling impacts bidirectional communications and mechanical interactions between endothelial cells and their extracellular matrix during vascular morphogenesis.
authors.affiliation: Institute of Developmental Biology and Cancer Research, University of Nice Sophia-Antipolis, CNRS-UMR6543, Centre Antoine Lacassagne, 33 Avenue de Valombrose, 06189 Nice, France. samantha.fernandez@univmed.fr
authors.forename: Samantha
authors.initials: S
authors.lastname: Fernandez-Sauze
authors.forename: Dominique
authors.initials: D
authors.lastname: Grall
authors.forename: Botond
authors.initials: B
authors.lastname: Cseh
authors.forename: Ellen
authors.initials: E
authors.lastname: Van Obberghen-Schilling