title: Preparation of lactose-modified cellulose films by a nonaqueous enzymatic reaction and their biofunctional characteristics as a scaffold for cell culture.
abstract: Enzymatic glyco-modification of transparent cellulose films with lactose was achieved by nonaqueous biocatalysis, and rat hepatocyte attachment behavior to the lactose-modified cellulose films was investigated. Regenerated cellulose films were incubated with lactose using a surfactant-enveloped cellulase in lithium chloride/dimethylacetamide solvent at 37 degrees C for 24 h, and lactose molecules were successfully introduced to the cellulose films. The initial cell adhesion on the lactose-modified cellulose films was superior to the original cellulose film. In the absence of serum, hepatocytes were significantly attached only on the lactose-modified cellulose films. This process was markedly suppressed by the addition of free lactose as an inhibitor. These results suggest that such cell adhesion proceeded through a direct interaction between galactose residues on the cellulose films and asialoglycoprotein receptors on the rat liver cell surface. This novel approach for surface glyco-modification of a cellulose matrix and its biofunctional properties are expected to provide potential application as a bioactive scaffold for cell culture engineering.
authors.affiliation: Department of Forest and Forest Products Sciences, Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University, 6-10-1 Hakozaki, Higashi-ku, Fukuoka 812-8581, Japan.
authors.forename: Kei
authors.initials: K
authors.lastname: Esaki
authors.forename: Shingo
authors.initials: S
authors.lastname: Yokota
authors.forename: Shizuka
authors.initials: S
authors.lastname: Egusa
authors.forename: Yuri
authors.initials: Y
authors.lastname: Okutani
authors.forename: Yukiko
authors.initials: Y
authors.lastname: Ogawa
authors.forename: Takuya
authors.initials: T
authors.lastname: Kitaoka
authors.forename: Masahiro
authors.initials: M
authors.lastname: Goto
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Wariishi