title: Hydrogenated amorphous carbon nanopatterned film designs drive human bone marrow mesenchymal stem cell cytoskeleton architecture.
abstract: The interaction between stem cells and biomaterials with nanoscale topography represents a main route in the roadmap for tissue engineering-based strategies. In this study, we explored the interface between human bone marrow-derived mesenchymal stem cells (hBM-MSCs) and hydrogenated amorphous carbon (a-C:H) film designed with uniform, groove, or grid nanopatterns. In either case, hBM-MSCs preserved growth rate and multi-differentiation properties, suggesting that the films were biocompatible and suitable for stem cell culture. hBM-MSCs responded to different nanopattern designs with specific changes of microtubule organization. In particular, the grid pattern induced a square-localized distribution of alpha-tubulin/actin fibers, whereas the groove pattern exerted a more dynamic effect, associated with microtubule alignment and elongation.
authors.affiliation: Department of Experimental Medicine and Biochemical Science, Section of Biochemistry and Molecular Biology, University of Perugia, Perugia, Italy.
authors.forename: Sabata
authors.initials: S
authors.lastname: Martino
authors.forename: Francesco
authors.initials: F
authors.lastname: D'Angelo
authors.forename: Ilaria
authors.initials: I
authors.lastname: Armentano
authors.forename: Roberto
authors.initials: R
authors.lastname: Tiribuzi
authors.forename: Manuela
authors.initials: M
authors.lastname: Pennacchi
authors.forename: Mariaserena
authors.initials: M
authors.lastname: Dottori
authors.forename: Samantha
authors.initials: S
authors.lastname: Mattioli
authors.forename: Auro
authors.initials: A
authors.lastname: Caraffa
authors.forename: Giuliano Giorgio
authors.initials: GG
authors.lastname: Cerulli
authors.forename: Josè Maria
authors.initials: JM
authors.lastname: Kenny
authors.forename: Aldo
authors.initials: A
authors.lastname: Orlacchio