A proposal for a coordinated effort for the determination of brainwide neuroanatomical connectivity in model organisms at a mesoscopic scale.
In this era of complete genomes, our knowledge of neuroanatomical circuitry remains surprisingly sparse. Such knowledge is critical, however, for both basic and clinical research into brain function. Here we advocate for a concerted effort to fill this gap, through systematic, experimental mapping of neural circuits at a mesoscopic scale of resolution suitable for comprehensive, brainwide coverage, using injections of tracers or viral vectors. We detail the scientific and medical rationale and briefly review existing knowledge and experimental techniques. We define a set of desiderata, including brainwide coverage; validated and extensible experimental techniques suitable for standardization and automation; centralized, open-access data repository; compatibility with existing resources; and tractability with current informatics technology. We discuss a hypothetical but tractable plan for mouse, additional efforts for the macaque, and technique development for human. We estimate that the mouse connectivity project could be completed within five years with a comparatively modest budget.
Bohland
Jason W
JW
Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, USA. bohland@cshl.edu
Wu
Caizhi
C
Barbas
Helen
H
Bokil
Hemant
H
Bota
Mihail
M
Breiter
Hans C
HC
Cline
Hollis T
HT
Doyle
John C
JC
Freed
Peter J
PJ
Greenspan
Ralph J
RJ
Haber
Suzanne N
SN
Hawrylycz
Michael
M
Herrera
Daniel G
DG
Hilgetag
Claus C
CC
Huang
Z Josh
ZJ
Jones
Allan
A
Jones
Edward G
EG
Karten
Harvey J
HJ
Kleinfeld
David
D
Kötter
Rolf
R
Lester
Henry A
HA
Lin
John M
JM
Mensh
Brett D
BD
Mikula
Shawn
S
Panksepp
Jaak
J
Price
Joseph L
JL
Safdieh
Joseph
J
Saper
Clifford B
CB
Schiff
Nicholas D
ND
Schmahmann
Jeremy D
JD
Stillman
Bruce W
BW
Svoboda
Karel
K
Swanson
Larry W
LW
Toga
Arthur W
AW
Van Essen
David C
DC
Watson
James D
JD
Mitra
Partha P
PP