Development of loop-mediated isothermal amplification assay for detection of Entamoeba histolytica.
A novel one-step, closed-tube, loop-mediated isothermal amplification (LAMP) assay for detecting Entamoeba histolytica, one of the leading causes of morbidity in developing countries, was developed. The sensitivity of the LAMP assay is 1 parasite per reaction. A total of 130 clinical samples were analyzed, and the results compared with those of conventional nested PCR to validate the practicability of this assay. No DNA was amplified from other diarrheal pathogens, such as other Entamoeba species, bacteria, and viruses. These results indicate that LAMP is a rapid, simple, and valuable diagnostic tool for epidemiological studies of amebiasis.
Liang
Shih-Yu
SY
Institute of Oral Biology, School of Dentistry, National Yang-Ming University, Taipei, Taiwan.
Chan
Yun-Hsien
YH
Hsia
Kan-Tai
KT
Lee
Jing-Lun
JL
Kuo
Ming-Chu
MC
Hwa
Kuo-Yuan
KY
Chan
Chi-Wen
CW
Chiang
Ting-Yi
TY
Chen
Jung-Sheng
JS
Wu
Fang-Tzy
FT
Ji
Dar-Der
DD