title: Potential use of quantum dots in flow cytometry.
abstract: QDs may offer significant advantages in environmental and bead-based applications where the target cells need to be discriminated above background fluorescence. We have examined the possible applications of QDs for flow cytometric measurements (FCM) by studying their excitation - emission spectra and their binding to paramagnetic beads. We labelled beads with either QDs or a commonly-used fluorochrome (FITC) and studied their fluorescence intensity by FCM. Flow cytometric comparisons indicated that the minimum fluorophore concentration required for detection of QDs above autofluorescent background was 100-fold less than for FITC.
authors.affiliation: Department of Chemistry and Biomolecular Sciences, Macquarie University, Sydney, Australia.
authors.forename: Raquel
authors.initials: R
authors.lastname: Ibáñez-Peral
authors.affiliation: Department of Chemistry and Biomolecular Sciences, Macquarie University, Sydney, Australia.|Department of Molecular Medicine & Pathology, University of Auckland Medical School, Auckland, New Zealand.
authors.forename: Peter L
authors.initials: PL
authors.lastname: Bergquist
authors.affiliation: Department of Earth Sciences, Macquarie University, Sydney, Australia.
authors.forename: Malcolm R
authors.initials: MR
authors.lastname: Walter
authors.affiliation: Department of Chemistry and Biomolecular Sciences, Macquarie University, Sydney, Australia.
authors.forename: Moreland
authors.initials: M
authors.lastname: Gibbs
authors.affiliation: Department of Physics, Macquarie University, Sydney, Australia.
authors.forename: Ewa M
authors.initials: EM
authors.lastname: Goldys
authors.affiliation: Department of Chemistry and Biomolecular Sciences, Macquarie University, Sydney, Australia.|School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, Australia.
authors.forename: Belinda
authors.initials: B
authors.lastname: Ferrari