title: Detection of survivin and p53 in human oral cancer: correlation with clinicopathologic findings.
abstract: BACKGROUND
  Survivin, an inhibitor of apoptosis, is overexpressed in cancer. It has been implicated in both prevention of apoptosis and cell cycle regulation. We investigated the distribution of antiapoptotic protein survivin in 29 oral squamous cell carcinoma (OSCC) and 16 oral premalignant lesions. It has been suggested that wild-type p53 represses survivin expression. Therefore, we investigated the status of p53 in relation to survivin to determine the potential involvement in oral tumorigenesis.
  
  
  METHODS
  Oral cancer tissues were freshly obtained at the time of surgery and classified as per general rules of head and neck cancer (TNM classification). Immunohistochemistry and reverse transcriptase-polymerase chain reaction were conducted to study the expression of survivin and p53. The Fisher's exact test was employed to determine the association of survivin and p53 with clinicopathologic parameters of the subjects being studied.
  
  
  RESULTS
  Positive staining for survivin was found in 72% OSCC and 44% oral premalignant lesions with no immunoreactions in the corresponding normal tissues. For p53, 59% OSCC, 38% premalignant lesions, and 14% normal tissues were positive. Importantly, about half of the p53-positive OSCC and premalignant tissues also showed survivin positivity (28% OSCC and 18% premalignant lesions). Further, it is observed that the number of survivin positive cells was significantly higher in the p53-positive group. Survivin is expressed in a varying proportion of cells, and in majority of patients it was localized in cytoplasm, whereas p53 is strictly restricted to the nucleus. The survivin expression levels in both primary OSCC and premalignant lesions were significantly higher than in normal oral tissues (OSCC, p < .0008; premalignant lesions, p < .04). No significant correlations between survivin and p53 expression with clinicopathologic parameters were found.
  
  
  CONCLUSIONS
  Frequent overexpression of apoptosis regulators, survivin and p53, in OSCC as well as in oral premalignant lesions were found. Overexpression of these 2 markers in premalignant lesions suggest a role in early stages of oral carcinogenesis.
authors.affiliation: Department of Biotechnology, Madhav Institute of Technology and Science, Race Course Road, Gwalior 474005, India.
authors.forename: Zakir
authors.initials: Z
authors.lastname: Khan
authors.forename: Ram P
authors.initials: RP
authors.lastname: Tiwari
authors.forename: Rita
authors.initials: R
authors.lastname: Mulherkar
authors.forename: Nand K
authors.initials: NK
authors.lastname: Sah
authors.forename: Godavarthi B K S
authors.initials: GB
authors.lastname: Prasad
authors.forename: Braj R
authors.initials: BR
authors.lastname: Shrivastava
authors.forename: Prakash S
authors.initials: PS
authors.lastname: Bisen