title: Resistance of common carp (Cyprinus carpio L.) to Cyprinid herpesvirus-3 is influenced by major histocompatibility (MH) class II B gene polymorphism.
abstract: The role of MH class II B (Cyca-DAB1-like) genes in resistance of common carp (Cyprinus carpio L.) to Cyprinid herpesvirus-3 (CyHV-3), also known as koi herpesvirus (KHV) was analysed. The material consisted of 934 fish from six carp crosses. Fish were challenged with CyHV-3 at an age of 7 and 10 months. During challenge experiments the peak of mortality caused by CyHV-3 was observed at days 8-12 p.i. and the overall cumulative mortality reached 79.9%. Among six Cyca-DAB1-like genotypes, revealed by PCR-RF-SSCP analysis, one genotype (E) was found associated with higher resistance to CyHV-3. Three other genotypes (B, H and J) could be linked to higher susceptibility to CyHV-3. Analysis of the alleles that compose the Cyca-DAB1-like genotypes linked one particular allele (Cyca-DAB1*05) to significantly increased, and two alleles (Cyca-DAB1*02 and Cyca-DAB1*06) to significantly decreased resistance to CyHV-3. Our data indicate that MH class II B genes could be used as potential genetic markers in breeding of common carp for resistance to this virus.
authors.affiliation: Polish Academy of Sciences, Institute of Ichthyobiology & Aquaculture in Gołysz, Zaborze, Chybie, Poland. krzysztof.rakus@fish.edu.pl
authors.forename: Krzysztof Ł
authors.initials: KŁ
authors.lastname: Rakus
authors.forename: Geert F
authors.initials: GF
authors.lastname: Wiegertjes
authors.forename: Mikołaj
authors.initials: M
authors.lastname: Adamek
authors.forename: Andrzej K
authors.initials: AK
authors.lastname: Siwicki
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Lepa
authors.forename: Ilgiz
authors.initials: I
authors.lastname: Irnazarow