Study on the physical properties of tissue-engineered blood vessels made by chemical cross-linking and polymer-tissue cross-linking.
In this study, we attempted to chemically cross-link decellularized blood vessel tissue and to perform cross-linking with a polymer in order to control its stability and functionalization. For this purpose, we cross-linked tissue by intrahelical, interhelical, and intermolecular cross-linking between the polymer and the collagen helix, which is a component of the native tissue. The intrahelically cross-linked tissue showed weaker stability against heat and degradation caused by collagenase compared to the interhelically cross-linked tissue. The tissue intermolecularly cross-linked with polymer showed the highest stability against heat and degradation caused by collagenase. The mechanical strength test showed that the Young's moduli were different for the intra/interhelically and intermolecularly cross-linked tissues, with the latter being stiffer. This is thought to be because the cross-linked polymer functions in the same way as elastin, whereas simple collagen cross-linking provides a supportive matrix that holds the collagen and elastin together.
Nam
Kwangwoo
K
Division of Biofunctional Molecules, Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, 2-3-10 Kanda-Surugadai, Chiyoda-ku, Tokyo, Japan.
Murakoshi
Ayako
A
Kimura
Tsuyoshi
T
Fujisato
Toshiya
T
Kitamura
Soichiro
S
Kishida
Akio
A