title: Folding, self-assembly, and bulk material properties of a de novo designed three-stranded beta-sheet hydrogel.
abstract: A de novo designed three-stranded beta-sheet (TSS1) has been prepared that undergoes temperature-induced folding and self-assembly to afford a network of beta-sheet rich fibrils that constitutes a mechanically rigid hydrogel. Circular dichroism and infrared spectroscopies show that TSS1 folds and self-assembles into a beta-sheet secondary structure in response to temperature. Rheological measurements show that the resulting hydrogels are mechanically rigid [at pH 9, G' = 1750-9000 Pa, and at pH 7.4, G' = 8500 Pa] and that the storage modulus can be modulated by temperature and peptide concentration. Nanoscale structure analysis by transmission electron microscopy and small angle neutron scattering indicate that the hydrogel network is comprised of fibrils that are about 3 nm in width, consistent with the width of TSS1 in the folded state. A unique property of the TSS1 hydrogel is its ability to shear-thin into a low viscosity gel upon application of shear stress and immediately recover its mechanical rigidity upon termination of stress. This attribute allows the hydrogel to be delivered via syringe to a target site with spatial and temporal resolution. Finally, experiments employing C3H10t1/2 mesenchymal stem cells seeded onto the hydrogel and incubated for 24 h indicate that the TSS1 hydrogel surface is noncytotoxic, supports cell adhesion, and allows cell migration.
authors.affiliation: Departments of Chemistry and Biochemistry, University of Delaware, Newark, Delaware, 19716, USA.
authors.forename: Ronak V
authors.initials: RV
authors.lastname: Rughani
authors.forename: Daphne A
authors.initials: DA
authors.lastname: Salick
authors.forename: Matthew S
authors.initials: MS
authors.lastname: Lamm
authors.forename: Tuna
authors.initials: T
authors.lastname: Yucel
authors.forename: Darrin J
authors.initials: DJ
authors.lastname: Pochan
authors.forename: Joel P
authors.initials: JP
authors.lastname: Schneider