Similar proteomic profiles of human mesenchymal stromal cells from different donors.
OBJECTIVE
Bone marrow (BM) stromal cells, also referred to as mesenchymal stromal cells (MSC), can be expanded ex vivo and are able to differentiate along multiple lineages, including chondrocytes, osteoblasts and adipocytes. MSC are known to secrete a number of cytokines and regulatory molecules implicated in different aspects of hematopoiesis, and seem to modulate the immune system. MSC appear to be promising candidates for cellular therapy associated with BM transplantation (BMT).


METHODS
We compared protein expression profiles of MSC cultures derived from different BM donors using two-dimensional (2-D) gel electrophoresis and matrix-assisted laser desorption/ionization time of flight (MALDI-TOF) tandem mass spectrometry (MS/MS), and compared mixed lymphocyte reaction (MLR) assays in the absence and presence of third-party human (h) MSC derived from different donors during the same culture passage.


RESULTS
In a window of observation (pH 4-7, molecular weight 10-220 kDa), about 172 protein spots were obtained in each 2-D gel, corresponding to 84 distinct proteins. A comparative analysis demonstrated a very similar proteomic profile of cells of the first passage derived from different donors, suggesting that these cells have the same expression pattern. Additionally, cells derived from different donors were equally able to inhibit lymphocyte proliferation.


CONCLUSIONS
These results encourage the use of third-party MSC in cellular therapies, as cells derived from different individuals seem to have the same proteomic pattern and exhibit functionally similar properties.
Lazzarotto-Silva
Carolina
C
Bone Marrow Transplantation Unit, National Cancer Institute, Rio de Janeiro, Brazil. clazza13@hotmail.com
Binato
Renata
R
Rocher
Bárbara Du
BD
Costa
Júlia Assunção Costa E
JA
Pizzatti
Luciana
L
Bouzas
Luis Fernando
LF
Abdelhay
Eliana
E