UVB radiation induced effects on cells studied by FTIR spectroscopy.
We have made a preliminary analysis of the results about the effects on tumoral cell line (lymphoid T cell line Jurkat) induced by UVB radiation (dose of 310 mJ/cm(2)) with and without a vegetable mixture. In the present study, we have used two techniques: Fourier transform infrared spectroscopy (FTIR) and flow cytometry. FTIR spectroscopy has the potential to provide the identification of the vibrational modes of some of the major compounds (lipid, proteins and nucleic acids) without being invasive in the biomaterials. The second technique has allowed us to perform measurements of cytotoxicity and to assess the percentage of apoptosis. We already studied the induction of apoptotic process in the same cell line by UVB radiation; in particular, we looked for correspondences and correlations between FTIR spectroscopy and flow cytometry data finding three highly probable spectroscopic markers of apoptosis (Pozzi et al. in Radiat Res 168:698-705, 2007). In the present work, the results have shown significant changes in the absorbance and spectral pattern in the wavenumber protein and nucleic acids regions after the treatments.
Di Giambattista
Lucia
L
Dipartimento di Fisica, Università di Roma Sapienza, Piazzale A. Moro 2, Rome, Italy. l.digiambattista@caspur.it
Grimaldi
P
P
Gaudenzi
S
S
Pozzi
D
D
Grandi
M
M
Morrone
S
S
Silvestri
I
I
Congiu Castellano
A
A