title: Characterization and application of a native lactic acid bacterium isolated from tannery fleshings for fermentative bioconversion of tannery fleshings.
abstract: Lactic acid bacteria (LAB) species isolated from limed and delimed tannery fleshings (TF) were evaluated for their fermentation efficiency and antibacterial property. The native LAB isolates efficiently fermented TF and resulted in a fermented mass with antioxidant properties, indicating their potential for effective eco-friendly bioconversion of TF. From among the LAB isolated, a proteolytic isolate showing better antimicrobial spectrum and reasonably good fermentation efficiency was identified as Enterococcus faecium HAB01 based on various biochemical and molecular tests. This isolate afforded a better degree of hydrolysis (81.36%) of TF than Pediococcus acidilactici (54.64%) that was previously reported by us. The bacteriocin produced by E. faecium was found to be antagonistic to several human pathogens including Listeria, Aeromonas, Staphylococcus and Salmonella. Further, E. faecium HAB01 bacteriocin was thermostable and had a molecular weight of around 5 kDa, apart from being stable at both acidic and alkaline conditions. The bacteriocin was unstable against proteases.
authors.affiliation: Department of Meat, Fish & Poultry Technology, Central Food Technological Research Institute, Karnataka, India.
authors.forename: Amit Kumar
authors.initials: AK
authors.lastname: Rai
authors.forename: N
authors.initials: N
authors.lastname: Bhaskar
authors.forename: Prakash M
authors.initials: PM
authors.lastname: Halami
authors.forename: K
authors.initials: K
authors.lastname: Indirani
authors.forename: P V
authors.initials: PV
authors.lastname: Suresh
authors.forename: N S
authors.initials: NS
authors.lastname: Mahendrakar