title: On the relationship between jetted inks and printed biopatterns: molecular-thin functional microarrays of glucose oxidase.
abstract: Arrays of circular spots of glucose oxidase have been obtained on functionalized silicon oxide by piezoelectric inkjet printing and the enzymatic activity toward glucose recognition has been monitored. The addition of glycerol to the molecular ink allows to obtain high spot definition and resolution (tens of micrometers wide; one molecule tall), but in spite of its well-known structural stabilizing properties, in dynamic conditions it may lead to increased protein stresses. The jetting voltage and pulse length have been found to be critical factors for both activity retention and pattern definition. High voltages and pulse lengths results in stress effects along with the loss of activity, which, at least in our experimental conditions, has been found to be recovered in time.
authors.affiliation: PLAST_ICs, Stradale Primosole 50, 95121, Catania, Italy.
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Arrabito
authors.forename: Chiara
authors.initials: C
authors.lastname: Musumeci
authors.forename: Venera
authors.initials: V
authors.lastname: Aiello
authors.forename: Sebania
authors.initials: S
authors.lastname: Libertino
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Compagnini
authors.forename: Bruno
authors.initials: B
authors.lastname: Pignataro