title: [Expression, characterization of recombinant human soluble BAFF secreted from CHO cell].
abstract: B cell-activating factor of the TNF family (BAFF) is critical for B cell maturation and survival. Here, we constructed a stable CHO cell line, in which the expression level of soluble form of BAFF (sBAFF) was raised from 0.13 microg/ml to 0.55 microg/ml. Purified recombinant sBAFF from these CHO cells not only bound to its receptors but also co-stimulated the proliferation of human peripheral blood B lymphocyte in vitro. These results provided us with a useful basis for further studies about sBAFF-related research.
authors.forename: H T
authors.initials: HT
authors.lastname: Wu
authors.forename: X M
authors.initials: XM
authors.lastname: Yan
authors.forename: Y L
authors.initials: YL
authors.lastname: Hu
authors.forename: Z Y
authors.initials: ZY
authors.lastname: Diao
authors.forename: J
authors.initials: J
authors.lastname: Wu
authors.forename: S Q
authors.initials: SQ
authors.lastname: Zhang