CELL-SELEX: Novel perspectives of aptamer-based therapeutics.
Aptamers, single stranded DNA or RNA molecules, generated by a method called SELEX (systematic evolution of ligands by exponential enrichment) have been widely used in various biomedical applications. The newly developed Cell-SELEX (cell based-SELEX) targeting whole living cells has raised great expectations for cancer biology, -therapy and regenerative medicine. Combining nanobiotechnology with aptamers, this technology opens the way to more sophisticated applications in molecular diagnosis. This paper gives a review of recent developments in SELEX technologies and new applications of aptamers.
Guo
Ke-Tai
KT
Department of Thoracic, Cardiac and Vascular surgery, University Hospital Tuebingen, Tuebingen, Germany, Calwerstr.7/1, D72076 Tuebingen, Germany.
Ziemer
Gerhard
G
Department of Thoracic, Cardiac and Vascular surgery, University Hospital Tuebingen, Tuebingen, Germany, Calwerstr.7/1, D72076 Tuebingen, Germany.
Paul
Angela
A
Department of Thoracic, Cardiac and Vascular surgery, University Hospital Tuebingen, Tuebingen, Germany, Calwerstr.7/1, D72076 Tuebingen, Germany.
Wendel
Hans P
HP
Department of Thoracic, Cardiac and Vascular surgery, University Hospital Tuebingen, Tuebingen, Germany, Calwerstr.7/1, D72076 Tuebingen, Germany.