title: Electrochemically deposited nanocomposite film of CS-Fc/Au NPs/GOx for glucose biosensor application.
abstract: A simple and controllable electrodeposition method is described to fabricate a homogeneous chitosan-ferrocene/Au nanoparticles/glucose oxidase (CS-Fc/Au NPs/GOx) nanocomposite film. The morphologies and electrochemistry of the nanocomposite film were investigated by using scanning electron microscopy (SEM) and electrochemical techniques including electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV), respectively. The ferrocene group (Fc) covalently bounded chitosan hybrid (CS-Fc) can effectively avoid the leakage of Fc and retain its electrochemical activity. Further immobilization of Au NPs into the CS-Fc matrix enhances both the charge-transport properties of the composite and bioaffinity to enzyme. Biosensor based on this CS-Fc/Au NPs/GOx film has advantages of fast response, excellent reproducibility and high stability. This biosensor shows a linear response to glucose in the concentration range from 0.02 to 8.66 mM with a detection limit of 5.6 microM at a signal-to-noise ratio of 3. The present method offers a facile way to fabricate biosensors and bioelectronic devices.
authors.affiliation: Department of Chemistry, Nanchang University, Nanchang 330031, China. jdqiu@ncu.edu.cn
authors.forename: Jian-Ding
authors.initials: JD
authors.lastname: Qiu
authors.forename: Rui
authors.initials: R
authors.lastname: Wang
authors.forename: Ru-Ping
authors.initials: RP
authors.lastname: Liang
authors.forename: Xing-Hua
authors.initials: XH
authors.lastname: Xia