title: Conjugation with alpha-linolenic acid improves cancer cell uptake and cytotoxicity of doxorubicin.
abstract: The synthetic DOX-LNA conjugate was characterized by proton nuclear magnetic resonance and mass spectrometry. In addition, the purity of the conjugate was analyzed by reverse-phase high-performance liquid chromatography. The cellular uptake, intracellular distribution, and cytotoxicity of DOX-LNA were assessed by flow cytometry, fluorescence microscopy, liquid chromatography/electrospray ionization tandem mass spectrometry, and the tetrazolium dye assay using the in vitro cell models. The DOX-LNA conjugate showed substantially higher tumor-specific cytotoxicity compared with DOX.
authors.affiliation: Department of Pharmacology, School of Pharmacy, Fourth Military Medical University, 17, Changle West Road, Xi'an, Shaan'xi 710032, China.
authors.forename: Meng-Lei
authors.initials: ML
authors.lastname: Huan
authors.forename: Si-Yuan
authors.initials: SY
authors.lastname: Zhou
authors.forename: Zeng-Hui
authors.initials: ZH
authors.lastname: Teng
authors.forename: Bang-le
authors.initials: BL
authors.lastname: Zhang
authors.forename: Xin-You
authors.initials: XY
authors.lastname: Liu
authors.forename: Jie-Pin
authors.initials: JP
authors.lastname: Wang
authors.forename: Qi-Bing
authors.initials: QB
authors.lastname: Mei