title: Conditioning vaccination site with irradiated MIP-3alpha-transfected tumor cells enhances efficacy of dendritic cell-based cancer vaccine.
abstract: Macrophage inflammation protein-3alpha (MIP-3alpha) is a chemokine expressed in inflamed tissue and capable of inducing migration of immature dendritic cells (DCs) or Langerhans cells. We postulated that conditioning vaccination sites with MIP-3alpha might enhance the efficacy of subsequently administered DC-based cancer vaccines. Our results demonstrate that subcutaneously injection of irradiated tumor cells expressing MIP-3alpha induces substantial cell infiltration to the injection site. Vaccination of irradiated tumor cells expressing MIP-3alpha followed by DCs pulsed with irradiated tumor cells can effectively suppress tumor growth in animals, which is significantly better than vaccination with irradiated MIP-3alpha-producing tumor cells or DCs pulsed with tumor cells alone. The protective effect was most evident when the MIP-3alpha-producing tumor cells and DC-based vaccines were injected at the same site. These results support the notion that this combination vaccination strategy might generate a more effective immune response to suppress the growth of tumor cells in animals.
authors.affiliation: National Institute of Cancer Research, National Health Research Institutes, 367 Sheng Li Road, Tainan 704, Taiwan.
authors.forename: Neng-Yao
authors.initials: NY
authors.lastname: Shih
authors.forename: Hui-Yu
authors.initials: HY
authors.lastname: Yang
authors.forename: Hui-Ting
authors.initials: HT
authors.lastname: Cheng
authors.forename: Yi-Mei
authors.initials: YM
authors.lastname: Hung
authors.forename: Yi-Chuan
authors.initials: YC
authors.lastname: Yao
authors.forename: Yun-Han
authors.initials: YH
authors.lastname: Zhu
authors.forename: Yu-Chen
authors.initials: YC
authors.lastname: Wu
authors.forename: Ko-Jiunn
authors.initials: KJ
authors.lastname: Liu