title: Analysis of cyclosporin A in dried blood spots using liquid chromatography tandem mass spectrometry.
abstract: Dried blood spot sampling is a promising and patient friendly alternative for venous sampling. A liquid chromatography tandem mass spectrometer assay was developed for analyzing cyclosporin A in dried blood spots. Linearity ranged from 25 to 1440 microg/L. Within and between run accuracy and precision were within limits. The developed assay has a negligible matrix-effect and a recovery of 97%. The dried blood spots were stable during a period of at least 17 days in the refrigerator. The developed assay is suitable for analyzing cyclosporin A in dried blood spots.
authors.affiliation: VU University Medical Center, Laboratory of Pharmacology and Pharmacy, HV, Amsterdam, Netherlands.
authors.forename: A J
authors.initials: AJ
authors.lastname: Wilhelm
authors.forename: J C G
authors.initials: JC
authors.lastname: den Burger
authors.forename: R M
authors.initials: RM
authors.lastname: Vos
authors.forename: A
authors.initials: A
authors.lastname: Chahbouni
authors.forename: A
authors.initials: A
authors.lastname: Sinjewel