[Effect of neuronal differentiation induced by nerve growth factor on the tolerance-dosage of ultraviolet radiation of PC12 cells].
OBJECTIVE
To evaluate the effect of neuronal differentiation induced by nerve growth factor (NGF) on the tolerance-dosage of ultraviolet radiation of PC12 Cells.


METHODS
Neuron-differentiated PC12 cells and untreated PC12 cells were exposed to different ultraviolet radiation dosage of 10, 30, 60, 80, 100, and 200 mJ/cm2. Cell survival rates were determined by MTT assay.


RESULTS
Neuron-differentiated PC12 cells had increased tolerance dose to ultraviolet radiation with noticeable apoptosis at the radiation dose of 100 mJ/cm2 in contrast to 30 mJ/cm2 for normal PC12 cells.


CONCLUSIONS
Neuronal differentiation exerts the effect of increasing the tolerance dose of PC12 cells to ultraviolet radiation.
Li
Dan-Hui
DH
Department of Dermatology, PUMC Hospital, CAMS and PUMC, Beijing 100730, China.
Xu
Hao-Xiang
HX
Song
Ping
P
Zuo
Ya-Gang
YG
Yan
Xiang-Hong
XH