title: Differential expression of aldolase, alpha tubulin and thioredoxin peroxidase in peripheral blood mononuclear cells from dengue fever and dengue hemorrhagic fever patients.
abstract: We determined the differential expression levels of proteins in peripheral blood mononuclear cells of patients with dengue fever (DF) and dengue hemorrhagic fever (DHF). Proteins were subjected to two-dimensional electrophoresis, mass spectrometry and Western blot analysis. We identified 8 proteins that were 2-fold or more up-regulated in patients compared to healthy control, three of which, aldolase, thioredoxin peroxidase and alpha tubulin, were related to dengue infection. Both thioredoxin peroxidase and alpha tubulin were over-expressed 4.9 and 3.3 times respectively in DHF compared to DF patients while aldolase was up-regulated 2.2 times in DF compared to DHF patients. Alpha tubulin and thioredoxin peroxidase have the potential to be utilized as biomarkers for DHF.
authors.affiliation: Department of Medical Microbiology, Faculty of Medicine, University Malaya, Kuala Lumpur, Malaysia.
authors.forename: Ravindran
authors.initials: R
authors.lastname: Thayan
authors.forename: Tan Lian
authors.initials: TL
authors.lastname: Huat
authors.forename: Lucy Lum Chai
authors.initials: LL
authors.lastname: See
authors.forename: Nor Shahidah
authors.initials: NS
authors.lastname: Khairullah
authors.forename: Rohana
authors.initials: R
authors.lastname: Yusof
authors.forename: Shamala
authors.initials: S
authors.lastname: Devi