title: Purification, crystallization and preliminary X-ray crystallographic analysis of xylose reductase from Candida tropicalis.
abstract: Xylose reductase (XR), which requires NADPH as a co-substrate, catalyzes the reduction of D-xylose to xylitol, which is the first step in the metabolism of D-xylose. The detailed three-dimensional structure of XR will provide a better understanding of the biological significance of XR in the efficient production of xylitol from biomass. XR of molecular mass 36.6 kDa from Candida tropicalis was crystallized using the hanging-drop vapour-diffusion method. According to X-ray diffraction data from C. tropicalis XR crystals at 2.91 A resolution, the unit cell belongs to space group P3(1) or P3(2). Preliminary analysis indicated the presence of four XR molecules in the asymmetric unit, with 68.0% solvent content.
authors.affiliation: Scientific Research Division, National Synchrotron Radiation Research Center, Hsinchu 30076, Taiwan.
authors.forename: Li Chun
authors.initials: LC
authors.lastname: Chen
authors.forename: Sheng Cih
authors.initials: SC
authors.lastname: Huang
authors.forename: Phimonphan
authors.initials: P
authors.lastname: Chuankhayan
authors.forename: Chung Der
authors.initials: CD
authors.lastname: Chen
authors.forename: Yen Chieh
authors.initials: YC
authors.lastname: Huang
authors.forename: Jeyaraman
authors.initials: J
authors.lastname: Jeyakanthan
authors.forename: Hsiao Fang
authors.initials: HF
authors.lastname: Pang
authors.forename: Lee Chung
authors.initials: LC
authors.lastname: Men
authors.forename: Yu Ching
authors.initials: YC
authors.lastname: Chen
authors.forename: Yu Kuo
authors.initials: YK
authors.lastname: Wang
authors.forename: Ming Yih
authors.initials: MY
authors.lastname: Liu
authors.forename: Tung Kung
authors.initials: TK
authors.lastname: Wu
authors.forename: Chun Jung
authors.initials: CJ
authors.lastname: Chen