Calcium-induced formation of subdomains in phosphatidylethanolamine-phosphatidylglycerol bilayers: a combined DSC, 31P NMR, and AFM study.
We study the effect of Ca(2+) on the lateral segregation of 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoethanolamine (POPE) and 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoglycerol (POPG) (3:1, mol/mol). Supported lipid bilayers (SLBs) were observed by atomic force microscopy (AFM). Since SLBs are formed from liposomes of POPE:POPG, we examined the effect of calcium on these suspensions by differential scanning calorimetry (DSC) and (31)P nuclear magnetic resonance spectroscopy ((31)P NMR). AFM images revealed the existence of two separated phases, the higher showing a region with protruding subdomains. Force spectroscopy (FS) was applied to clarify the nature of each phase. The values of breakthrough force (F(y)), adhesion force (F(adh)), and height extracted from the force curves were assigned to the corresponding gel (L(beta)) and fluid (L(alpha)) phase. The endotherms obtained by DSC suggest that, in the presence of Ca(2+), phase separation already exists in the suspensions of POPE:POPG used to form SLBs. Due to the temperature changes applied during preparation of SLBs a (31)P NMR study was performed to assess the lamellar nature of the samples before spreading them onto mica. With in situ AFM experiments we showed that the binding of Ca(2+) to POPG-enriched domains only induces the formation of subdomains in the L(beta) phase.
Picas
Laura
L
Departament de Fisicoquimica, Facultat de Farmacia, Universitat de Barcelona (UB), and Institut de Nanociencia i Nanotecnologia de la Universitat de Barcelona (IN2UB), E-08028 Barcelona, Spain.
Montero
M Teresa
MT
Morros
Antoni
A
Cabañas
Miquel E
ME
Seantier
Bastien
B
Milhiet
Pierre-Emmanuel
PE
Hernández-Borrell
Jordi
J