title: Prognostic significance of p53 codon 72 polymorphism differs with race in colorectal adenocarcinoma.
abstract: OBJECTIVE
  Several studies have examined the prognostic value of the codon 72 polymorphism of the p53 gene in colorectal adenocarcinoma, but none have addressed patient race/ethnicity. Therefore, this study assessed the prognostic value of this polymorphism in African American and Caucasian colorectal adenocarcinoma patients separately.
  
  
  METHODS
  Colorectal adenocarcinomas from 137 African Americans and 236 non-Hispanic Caucasians were assessed for p53 mutations and genotyped for the codon 72 polymorphism. The phenotypes were correlated with p53 mutational status, clinicopathologic features, and patient survival using the chi(2) test and Kaplan-Meier and Cox regression models.
  
  
  RESULTS
  The incidence of p53 mutations was similar in African American and Caucasian patients (50% versus 54%, respectively); however, the homozygous Pro72 allele frequency was higher in African Americans (17%) as compared with Caucasians (7%). In contrast, the homozygous Arg72 allele frequency was higher in Caucasians (36%) than in African Americans (19%). In African Americans but not Caucasians, the Pro/Pro phenotype significantly correlated with a higher incidence of missense p53 mutations and with nodal metastasis. African Americans, but not Caucasians, with the Pro/Pro phenotype had significantly higher mortality (log-rank P = 0.005 versus. P = 0.886) and risk of death due to colorectal adenocarcinoma (hazard ratio, 2.15; 95% confidence interval, 1.02-4.53 versus hazard ratio, 1.60; 95% confidence interval, 0.69-3.18) than those with the phenotype Arg/Arg or Arg/Pro.
  
  
  CONCLUSIONS
  The higher frequency of the Pro/Pro phenotype of p53 in African American patients with colorectal adenocarcinoma is associated with an increased incidence of p53 mutations, with advanced tumor stage, and with short survival.
authors.affiliation: Department of Pathology, University of Alabama at Birmingham, Birmingham, Alabama 35294-7331, USA.
authors.forename: Venkat R
authors.initials: VR
authors.lastname: Katkoori
authors.forename: Xu
authors.initials: X
authors.lastname: Jia
authors.forename: Chandrakumar
authors.initials: C
authors.lastname: Shanmugam
authors.forename: Wen
authors.initials: W
authors.lastname: Wan
authors.forename: Sreelatha
authors.initials: S
authors.lastname: Meleth
authors.forename: Harvey
authors.initials: H
authors.lastname: Bumpers
authors.forename: William E
authors.initials: WE
authors.lastname: Grizzle
authors.forename: Upender
authors.initials: U
authors.lastname: Manne