Stimulation of guanylyl cyclase-D by bicarbonate.
Guanylyl cyclases (GCs) catalyze the conversion of GTP to the second messenger cGMP. While some transmembrane GCs are receptors for extracellular ligands, other transmembrane GCs such as retinal-specific GC-E and GC-F are stimulated by cellular proteins. GC-D is expressed in a special group of olfactory sensory neurons. However, the direct regulatory mechanism of GC-D activity is not completely understood. Here we have demonstrated that bicarbonate directly increases the activity of purified GC-D. Bicarbonate also increases the cGMP levels in cells expressing GC-D. These results identify bicarbonate as a small molecule that regulates GC-D.
Guo
Dagang
D
Department of Physiology, Cornell University Weill Medical College, New York, New York 10065, USA.
Zhang
J Jillian
JJ
Huang
Xin-Yun
XY