title: The effect of photodynamic treatment combined with antibiotic action or host defence mechanisms on Staphylococcus aureus biofilms.
abstract: Staphylococcus aureus is one of the most important etiological agents of infections associated with medical devices. This is in part due to the ability of the organism to form biofilm, which provides a microenvironment that protects from attack by the host's immune system and by antibiotics. In this study we examined the structure of polysaccharide intercellular adhesin (PIA)-dependent or protein-based S. aureus biofilms. We defined new strategies aimed at treatment of mature established biofilms using photodynamic treatment (PDT) combined with chemotherapy or phagocytosis. Significant inactivation of bacteria was observed when structurally distinct biofilms were exposed to the cationic porphyrin, tetra-substituted N-methyl-pyridyl-porphine (TMP), and simultaneously to visible light. Moreover, PDT-treated biofilms exposed to vancomycin or subjected to the phagocytic action of whole blood resulted in their almost complete eradication. The drastic reduction in staphylococcal survival and the disruption of biofilms were confirmed by confocal laser scanning microscopy and scanning electron microscopy. The results suggest that PDT combined with vancomycin and the host defences may be a useful approach for the inactivation of staphylococcal biofilms adhering to medical implant surfaces.
authors.affiliation: University of Pavia, Department of Biochemistry, Viale Taramelli 3/b, Pavia 27100, Italy.
authors.forename: Antonella
authors.initials: A
authors.lastname: Di Poto
authors.forename: Maria S
authors.initials: MS
authors.lastname: Sbarra
authors.forename: Giulio
authors.initials: G
authors.lastname: Provenza
authors.forename: Livia
authors.initials: L
authors.lastname: Visai
authors.forename: Pietro
authors.initials: P
authors.lastname: Speziale