title: [Molecular pathways of germ cell apoptosis following testicular torsion in rats].
abstract: OBJECTIVE
  To investigate the molecular mechanism of germ cell apoptosis following testicular torsion in rats.
  
  
  METHODS
  Healthy male Sprague-Dawley rats (n = 16) were equally randomized into a control and a torsion group and the models of testicular torsion (720 degrees 2 h) were established. Twenty-four hours later, the apoptosis and count of germ cells were determined by flow cytometry, the expressions of Bax, Fas and Fas ligand (FasL) mRNA semiquantitatively analyzed by RT-PCR and the cytochrome C release detected by Western blot.
  
  
  RESULTS
  Compared with the control group, there was an obvious increase in the number of apoptotic germ cells, a marked decrease in that of haploid and tetraploid cells and significantly up-regulated expressions of Bax and Fas/FasL mRNA in the torsion group (P <0.01). The Western blot analysis showed that the cytochrome C release was remarkably increased 24 hours after the detorsion. There were significant differences between the two groups (P <0.01).
  
  
  CONCLUSIONS
  There are two major signaling pathways of cell apoptosis following testicular torsion, intercellular and intracellular. Up-regulated expressions of the apoptosis-related molecules Bax and Fas/FasL and increased cytochrome C release may play an important role in germ cell apoptosis following testicular torsion in rats.
authors.affiliation: Department of Urology, Zhongshan Hospital, Xiamen University, Xiamen, Fujian 361004, China. lzmwhdx@126.com
authors.forename: Zi-Ming
authors.initials: ZM
authors.lastname: Liu
authors.forename: Xin-Min
authors.initials: XM
authors.lastname: Zheng