title: Development of an in-capillary approach to nanoscale automated in vitro cytochromes p450 assays.
abstract: A method to perform nanoscale automated CYP450-based drug metabolism studies using a capillary as a reaction vessel is described. In-capillary assays consumed only approximately 30 nL of recombinant human CYP450 solution. Ultrafast analysis of substrates and metabolites was achieved off-line by ultrahigh-pressure liquid chromatography coupled to mass spectrometry. This approach was successfully applied to qualitative metabolism studies of six major CYP450 isozymes and CYP2D6 inhibition experiments.
authors.affiliation: School of Pharmaceutical Sciences, University of Geneva and University of Lausanne, Boulevard d'Yvoy 20, 1211 Geneva 4, Switzerland.
authors.forename: Raul
authors.initials: R
authors.lastname: Nicoli
authors.forename: Raffaele
authors.initials: R
authors.lastname: Curcio
authors.forename: Serge
authors.initials: S
authors.lastname: Rudaz
authors.forename: Jean-Luc
authors.initials: JL
authors.lastname: Veuthey