Outcome of intracytoplasmic sperm injection with and without polar body diagnosis of oocytes.
OBJECTIVE
To compare the reproductive outcome of women undergoing intracytoplasmic sperm injection (ICSI) with or without polar body diagnosis of oocytes.


METHODS
Nonrandomized retrospective study.


METHODS
University-based human genetic institute in collaboration with a private fertility center.


METHODS
Six hundred seven women undergoing ICSI with polar body diagnosis and 591 women undergoing ICSI without polar body diagnosis at the same time in the same fertility center.


METHODS
Polar body testing of ICSI oocytes by five-color fluorescence in situ hybridization.


METHODS
Pregnancy rate (positive fetal heartbeats) and live-birth rate (of at least one child).


RESULTS
The pregnancy and live-birth rates were significantly lower in women undergoing ICSI with polar body diagnosis than in women without polar body diagnosis. The negative effects of polar body diagnosis were evident in all analyzed subgroups, that is, women of different age groups, with one ICSI cycle, with transfer of a high-quality embryo, and with male factor infertility as indication for ICSI.


CONCLUSIONS
Within the legal restrictions of the German embryo protection law aneuploidy testing of oocytes may not improve reproductive outcome.
Haaf
Thomas
T
Institute for Human Genetics, Johannes Gutenberg University, Langenbeckstrasse 1, Bldg. 601, 55131 Mainz, Germany. haaf@humgen.klinik.uni-mainz.de
Tresch
Achim
A
Lambrecht
Anne
A
Grossmann
Bärbel
B
Schwaab
Eva
E
Khanaga
Omar
O
Hahn
Thomas
T
Schorsch
Martin
M