Resistance of polysaccharide coatings to proteins, hematopoietic cells, and marine organisms.
The interaction of covalently coupled hyaluronic acid, alginic acid, and pectic acid with proteins, cells (hematopoietic KG1a and Jurkat cells), and marine organisms (algal zoospores and barnacle cypris larvae) is compared. In contrast to cells and proteins for which such polysaccharide coatings are known for their antiadhesive properties, marine algal spores and barnacle cyprids were able to colonize the surfaces. Of the three polysaccharides, hyaluronic acid showed the lowest settlement of both Ulva zoopores and barnacles. Photoelectron spectroscopy reveals that the polysaccharide coatings tend to bind bivalent ions, such as calcium, from salt water. Such pretreatment with a high salinity medium significantly changes the protein and hematopoietic cell resistance of the surfaces. Complexation of bivalent ions is therefore considered as one reason for the decreased resistance of polysaccharide coatings when applied in the marine environment.
Cao
Xinyu
X
Applied Physical Chemistry, University of Heidelberg, 69120 Heidelberg, Germany.
Pettit
Michala E
ME
Conlan
Sheelagh L
SL
Wagner
Wolfgang
W
Ho
Anthony D
AD
Clare
Anthony S
AS
Callow
James A
JA
Callow
Maureen E
ME
Grunze
Michael
M
Rosenhahn
Axel
A