High hydrostatic pressure tolerance of four different anhydrobiotic animal species.
High hydrostatic pressure (HHP) can induce physical changes in DNA, proteins, and lipids, causing lethal or sublethal damage to organisms. However, HHP tolerance of animals has not been studied sufficiently. In this study, HHP tolerance of four species of invertebrate anhydrobiotes (the tardigrade Milnesium tardigradum, a nematode species in the family Plectidae, larvae of Polypedilum vanderplanki, and cysts of Artemia franciscana), which have the potential to enter anhydrobiosis upon desiccation, were investigated by exposing them to 1.2 GPa for 20 minutes. This exposure killed the anhydrobiotes in their ordinary hydrated state, but did not affect their survival in the anhydrobiotic state. The results indicated that the hydrated anhydrobiotes were vulnerable to HHP, but that HHP of 1.2 GPa was not sufficient to kill them in anhyrdobiosis.
Horikawa
Daiki D
DD
Graduate School of Science, The University of Tokyo, Hongo 7-3-1, Bunkyo-ku, Tokyo 113-0033, Japan. daiki.horikawa-1@nasa.gov
Iwata
Ken-Ichi
K
Kawai
Kiyoshi
K
Koseki
Shigenobu
S
Okuda
Takashi
T
Yamamoto
Kazutaka
K