title: Marinamoeba thermophila, a new marine heterolobosean amoeba growing at 50 degrees C.
abstract: Two amoeba strains were isolated from marine sediment taken at the same place with 18 months interval from a region of the sea floor heated by extended submarine hot springs and fumaroles. These thermophilic amoebae grow at temperatures up to 50 degrees C. Sequences of the internal transcribed spacer demonstrated that the two strains belong to the same species and are different from any genus for which sequences are known. Phylogeny using small subunit ribosomal RNA places the amoeba in the Heterolobosea. Their closest relatives are the hypersaline flagellate Pleurostomum flabellatum and the hypersaline amoeba Tulamoeba peronaphora. The freshwater amoeboflagellate genera Naegleria and Willaertia belong to the same phylogenetic clade in the Vahlkampfiidae. The new marine species does not transform into flagellates. It forms cysts, which are round to ellipsoidal with few pores. Because of their unique place in the molecular phylogenetic tree, and because there is no morphologically identical species found in the literature, these isolates are considered to be a new species and a new genus, Marinamoeba thermophila.
authors.affiliation: Research Unit for Tropical Diseases, de Duve Institute, Brussels, Belgium. johan.dejonckheere@uclouvain.be
authors.forename: Johan F
authors.initials: JF
authors.lastname: De Jonckheere
authors.forename: Manuela
authors.initials: M
authors.lastname: Baumgartner
authors.forename: Fred R
authors.initials: FR
authors.lastname: Opperdoes
authors.forename: Karl O
authors.initials: KO
authors.lastname: Stetter