A new developmental toxicity test for pelagic fish using anchoveta (Engraulis ringens J.).
A series of six 96-h static bioassays were performed to validate the use of anchoveta (Engraulis ringens) embryos as test organisms for ecotoxicological studies. The standardization protocol utilized potassium dichromate (K(2)Cr(2)O(7)) as a reference toxicant and egg mortality as the endpoint. The results indicated that the mean sensitivity of anchoveta embryos to potassium dichromate was 156.1 mg L(-1) (range: 131-185 mg L(-1)). The statistical data analysis showed high homogeneity in LC50 values among bioassays (variation coefficient = 11.02%). These results demonstrated that the protocol and handling procedures implemented for the anchoveta embryo bioassays comply with international standards for intra-laboratory precision. After secondary treatment, an effluent from a modern Kraft pulp mill was tested for E. ringens embryo toxicity, finding no significant differences from the controls.
Llanos-Rivera
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Programa de Investigación Marina de Excelencia (PIMEX-Nueva Aldea), Facultad de Ciencias Naturales y Oceanográficas, Universidad de Concepción, Casilla160-C, Concepción, Chile. alllanos@udec.cl
Castro
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LR
Silva
J
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Bay-Schmith
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