Potential use of quantum dots in flow cytometry.
QDs may offer significant advantages in environmental and bead-based applications where the target cells need to be discriminated above background fluorescence. We have examined the possible applications of QDs for flow cytometric measurements (FCM) by studying their excitation - emission spectra and their binding to paramagnetic beads. We labelled beads with either QDs or a commonly-used fluorochrome (FITC) and studied their fluorescence intensity by FCM. Flow cytometric comparisons indicated that the minimum fluorophore concentration required for detection of QDs above autofluorescent background was 100-fold less than for FITC.
Ibáñez-Peral
Raquel
R
Department of Chemistry and Biomolecular Sciences, Macquarie University, Sydney, Australia.
Bergquist
Peter L
PL
Department of Chemistry and Biomolecular Sciences, Macquarie University, Sydney, Australia.|Department of Molecular Medicine & Pathology, University of Auckland Medical School, Auckland, New Zealand.
Walter
Malcolm R
MR
Department of Earth Sciences, Macquarie University, Sydney, Australia.
Gibbs
Moreland
M
Department of Chemistry and Biomolecular Sciences, Macquarie University, Sydney, Australia.
Goldys
Ewa M
EM
Department of Physics, Macquarie University, Sydney, Australia.
Ferrari
Belinda
B
Department of Chemistry and Biomolecular Sciences, Macquarie University, Sydney, Australia.|School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, Australia.