title: Outcome of intracytoplasmic sperm injection with and without polar body diagnosis of oocytes.
abstract: OBJECTIVE
  To compare the reproductive outcome of women undergoing intracytoplasmic sperm injection (ICSI) with or without polar body diagnosis of oocytes.
  
  
  METHODS
  Nonrandomized retrospective study.
  
  
  METHODS
  University-based human genetic institute in collaboration with a private fertility center.
  
  
  METHODS
  Six hundred seven women undergoing ICSI with polar body diagnosis and 591 women undergoing ICSI without polar body diagnosis at the same time in the same fertility center.
  
  
  METHODS
  Polar body testing of ICSI oocytes by five-color fluorescence in situ hybridization.
  
  
  METHODS
  Pregnancy rate (positive fetal heartbeats) and live-birth rate (of at least one child).
  
  
  RESULTS
  The pregnancy and live-birth rates were significantly lower in women undergoing ICSI with polar body diagnosis than in women without polar body diagnosis. The negative effects of polar body diagnosis were evident in all analyzed subgroups, that is, women of different age groups, with one ICSI cycle, with transfer of a high-quality embryo, and with male factor infertility as indication for ICSI.
  
  
  CONCLUSIONS
  Within the legal restrictions of the German embryo protection law aneuploidy testing of oocytes may not improve reproductive outcome.
authors.affiliation: Institute for Human Genetics, Johannes Gutenberg University, Langenbeckstrasse 1, Bldg. 601, 55131 Mainz, Germany. haaf@humgen.klinik.uni-mainz.de
authors.forename: Thomas
authors.initials: T
authors.lastname: Haaf
authors.forename: Achim
authors.initials: A
authors.lastname: Tresch
authors.forename: Anne
authors.initials: A
authors.lastname: Lambrecht
authors.forename: Bärbel
authors.initials: B
authors.lastname: Grossmann
authors.forename: Eva
authors.initials: E
authors.lastname: Schwaab
authors.forename: Omar
authors.initials: O
authors.lastname: Khanaga
authors.forename: Thomas
authors.initials: T
authors.lastname: Hahn
authors.forename: Martin
authors.initials: M
authors.lastname: Schorsch