title: Prevalence of Chlamydia trachomatis and herpes simplex virus 2 in cervical carcinoma associated with human papillomavirus detected in paraffin-sectioned samples.
abstract: OBJECTIVE
  The aim of the study was to evaluate the frequency of occurrence of HPV and co-infection: Chlamydia (C.) trachomatis and HSV-2 in cervical cancer.
  
  
  METHODS
  The study group consisted of 570 paraffin-sectioned samples of patients with cervical cancer. In order to identify viral and bacterial DNA in DNA isolated from archival, postoperative material, PCR analysis was performed using starters complementary to various types of HPV, HSV-2 and C. trachomatis.
  
  
  RESULTS
  In patients with squamous cell cervical cancer the presence of 33 types of HPV was found in 90% (468/520). HPV 16 infections occurred in 69.4% (325/468), while HPV 18 infections were present in 30.5% (143/468) of cases. In the control group C. trachomatis and HSV-2 were observed in four cases (4/50), which constitute 8.0%. In the tissue sections from patients with squamous cell cervical carcinoma, C. trachomatis was identified in 26% (135/520) and HSV-2 in 28% (145/520). In the group of patients with adenocarcinoma C. trachomatis infections were found in 24% (12/50) and herpes virus was identified in 30% (15/50). Statistically significantly higher frequency of occurrence of HSV-2 and C. trachomatis was observed in paraffin-sectioned samples for patients with invasive cervical cancer compared to the control group, without neoplastic lesions (p < 0.05). No correlation was found between frequency of occurrence of HPV and C. trachomatis and of HPV and HSV-2 detected in paraffin-sectioned samples for cervical carcinoma.
authors.affiliation: Department of Obstetrics and Gynecology, Medical University, Lublin, Poland. anna.kwasniewska@am.lublin.pl
authors.forename: A
authors.initials: A
authors.lastname: Kwaśniewska
authors.forename: E
authors.initials: E
authors.lastname: Korobowicz
authors.forename: M
authors.initials: M
authors.lastname: Zdunek
authors.forename: M
authors.initials: M
authors.lastname: Skoczyński
authors.forename: W
authors.initials: W
authors.lastname: Kwaśniewski
authors.forename: J
authors.initials: J
authors.lastname: Daniłoś
authors.forename: A
authors.initials: A
authors.lastname: Goździcka-Józefiak