title: Heterogeneous nuclear ribonucleoprotein L Is a subunit of human KMT3a/Set2 complex required for H3 Lys-36 trimethylation activity in vivo.
abstract: The presence of histone H3 lysine 36 methylation (H3K36me) correlates with actively transcribed genes. In yeast, histone H3K36me mediated by KMT3 (also known as Set2) recruits a histone deacetylase complex, Rpd3s, to ensure the fidelity of transcription initiation. We report the purification of human KMT3a (also known as HYPB or hSet2) complex and the identification of a novel, higher eukaryotic specific subunit, heterogeneous nuclear ribonucleoprotein L (HnRNP-L). Interestingly, although KMT3a has intrinsic activity in vitro, HnRNP-L is essential in vivo. Moreover, KMT3a generates mono-, di-, and trimethylated products in vitro, but RNA interference against KMT3a or HnRNP-L down-regulates exclusively the H3K36me3 mark in vivo.
authors.affiliation: State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultural University, Beijing 100094, China.
authors.forename: Wen
authors.initials: W
authors.lastname: Yuan
authors.forename: Jingwei
authors.initials: J
authors.lastname: Xie
authors.forename: Chengzu
authors.initials: C
authors.lastname: Long
authors.forename: Hediye
authors.initials: H
authors.lastname: Erdjument-Bromage
authors.forename: Xiaojun
authors.initials: X
authors.lastname: Ding
authors.forename: Yong
authors.initials: Y
authors.lastname: Zheng
authors.forename: Paul
authors.initials: P
authors.lastname: Tempst
authors.forename: She
authors.initials: S
authors.lastname: Chen
authors.forename: Bing
authors.initials: B
authors.lastname: Zhu
authors.forename: Danny
authors.initials: D
authors.lastname: Reinberg