A novel co-polymer based on hydroxypropyl alpha-cyclodextrin conjugated to low molecular weight polyethylenimine as an in vitro gene delivery vector.
A novel co-polymer based on 2-hydroxypropyl-alpha-cyclodextrin cross-linked by low molecular weight polyethylenimine was synthesized as a gene delivery vector. The copolymer could bind and condense DNA tightly. It showed lower cytotoxicity than PEI 25kDa in SK-BR-3 cells. Transfection efficiency was increased over 5.5-fold higher than PEI 25 kDa in SK-BR-3 cells in complete serum medium. It is a potential candidate vector for gene therapy.
Huang
Hongliang
H
School of Life Science, Guangdong Pharmaceutical University, Guangzhou Higher Education Mega Center, Guangzhou, P. R. China.
Yu
Hai
H
Institute of Immunology, Zhejiang University, Yuhangtang Road 388, Hangzhou, P. R. China.
Li
Da
D
Institute of Immunology, Zhejiang University, Yuhangtang Road 388, Hangzhou, P. R. China.
Liu
Yang
Y
Institute of Immunology, Zhejiang University, Yuhangtang Road 388, Hangzhou, P. R. China.
Shen
Fenping
F
Institute of Immunology, Zhejiang University, Yuhangtang Road 388, Hangzhou, P. R. China.
Zhou
Jun
J
Institute of Chemical Biology and Pharmaceutical Chemistry, Zhejiang University, Tianmushan Road 148, Hangzhou, P. R. China.
Wang
Qingqing
Q
Institute of Immunology, Zhejiang University, Yuhangtang Road 388, Hangzhou, P. R. China.
Tang
Guping
G
Institute of Chemical Biology and Pharmaceutical Chemistry, Zhejiang University, Tianmushan Road 148, Hangzhou, P. R. China.