title: Proteomic analysis of a filamentous fungal endophyte using EST datasets.
abstract: Proteomic analysis of many species of fungi, particularly filamentous fungi, is difficult due to the lack of publicly available genome sequence data and the problems associated with cross-species comparisons. Furthermore, the detection of fungal proteins in biological systems where there are a greater number of proteins present from other eukaryote species provides additional challenges. We present an EST-based approach for identifying proteins from a fungal endophyte of temperate grasses and demonstrate that this method is well suited for fungi with minimal sequence data.
authors.affiliation: AgResearch Ltd., Grasslands Research Centre, Palmerston North, New Zealand.
authors.forename: Shalome A
authors.initials: SA
authors.lastname: Bassett
authors.forename: Judy J
authors.initials: JJ
authors.lastname: Bond
authors.forename: Fiona Y S
authors.initials: FY
authors.lastname: Kwan
authors.forename: Alan F
authors.initials: AF
authors.lastname: McCulloch
authors.forename: Paul A
authors.initials: PA
authors.lastname: Haynes
authors.forename: Richard D
authors.initials: RD
authors.lastname: Johnson
authors.forename: Gregory T
authors.initials: GT
authors.lastname: Bryan
authors.forename: T William
authors.initials: TW
authors.lastname: Jordan