Vitrification of immature mouse oocyte using stepwise equilibration before or after in vitro maturation.
Immature mouse oocytes before or after in vitro maturation (IVM) were vitrified by the use of either conventional one-step or three-step equilibration. Although vitrification after IVM using the stepwise method does not affect the cleavage rate, the early embryonic development was profoundly impaired in both protocols.
Jee
Byung Chul
BC
Department of Obstetrics and Gynecology, Seoul National University Bundang Hospital, Seongnam, South Korea.
Chen
Hai Ying
HY
Department of Obstetrics and Gynecology, McGill University, Montreal, Quebec, Canada.
Chian
Ri-Cheng
RC
Department of Obstetrics and Gynecology, McGill University, Montreal, Quebec, Canada. Electronic address: richeng.chian@muhc.mcgill.ca.
Suh
Chang Suk
CS
Department of Obstetrics and Gynecology, Seoul National University Bundang Hospital, Seongnam, South Korea; Department of Obstetrics and Gynecology, College of Medicine, Seoul National University, Seoul, South Korea; Institute of Reproductive Medicine and Population, Seoul National University, Seoul, South Korea.
Kim
Seok Hyun
SH
Department of Obstetrics and Gynecology, College of Medicine, Seoul National University, Seoul, South Korea; Institute of Reproductive Medicine and Population, Seoul National University, Seoul, South Korea.
Moon
Shin Yong
SY
Department of Obstetrics and Gynecology, College of Medicine, Seoul National University, Seoul, South Korea; Institute of Reproductive Medicine and Population, Seoul National University, Seoul, South Korea.