title: Separation of viable histamine-producing bacteria from yellowtail meat components by density gradient centrifugation.
abstract: Quantitative separation of live cells from food samples is essential for non-culture methods to be validated. In this viewpoint, the feasibility of density gradient centrifugation (DGC) was demonstrated for the first time using samples of yellowtail meat to which Morganella morganii, a histamine producing bacterium had been added. Using a Ficoll density gradient from 50 to 10 w/v % with 10 w/v % steps, meat-free fractions of M. morganii cells were collected in 20-50 w/v % layers. The total cell collection rate ranged from 73-86 % irrespective of the cell density in the range 10(2)-10(6) cells/200 microl.
authors.affiliation: Japan Food Research Laboratories, 52-1, Motoyoyogi-cho, Shibuya-ku, Tokyo 151-0062, Japan.
authors.forename: Emiko
authors.initials: E
authors.lastname: Araki
authors.forename: Kodai
authors.initials: K
authors.lastname: Takayama
authors.forename: Mikako
authors.initials: M
authors.lastname: Saito
authors.forename: Hideaki
authors.initials: H
authors.lastname: Matsuoka