title: The mode of the antifungal activity of gemini-pyridinium salt against yeast.
abstract: The gemini quaternary salt (gemini-QUAT) containing two pyridinium residues per molecule, 3,3'-(2,7-dioxaoctane) bis (1-decylpyridinium bromide) (3DOBP-4,10), exerted fungicidal activity against Saccharomyces cerevisiae accompanied by respiration inhibition and the cytoplasmic material leakage of ATP, magnesium, and potassium ions. We previously found that gemini-QUAT exerted bacterioclastic action against Escherichia coli by causing the rapid and abundant leakage of turbid materials from the cells. In addition, the first stage of the bacterioclastic action was the leakage of magnesium ions, outer membrane protein E, ATP, and lipopolysaccharides. Here, we investigated how the gemini-QUAT 3DOBP-4,10 exerts fungicidal action against S. cerevislae. The results showed that that > or = 0.4 microM 3DOBP-4,10 stopped respiration and that > or = 3.0, 1.0 and 1.0 microM caused the leakage of cytoplasmic components ATP, magnesium and potassium ions, respectively. Scanning and transmission electron micrographs showed a preserved cell wall structure, whereas intracellular organelles were destroyed in cells incubated with 3DOBP-4,10. We postulated that 3DOBP-4,10 exerts its fungicidal action against S. cerevisiae not through cell wall destruction and protein leakage, but rather by penetrating the cell wall and disrupting the membranes of organelles.
authors.affiliation: Department of Biological Science and Technology, Biosystems Engineering, Institute of Technology and Science, The University of Tokushima Graduate School, 2-1, Minamijosanjima-cho, Tokushima 770-8506, Japan.
authors.forename: Akihiro
authors.initials: A
authors.lastname: Shirai
authors.forename: Tomoko
authors.initials: T
authors.lastname: Sumitomo
authors.forename: Mayuko
authors.initials: M
authors.lastname: Kurimoto
authors.forename: Hideaki
authors.initials: H
authors.lastname: Maseda
authors.forename: Hiroki
authors.initials: H
authors.lastname: Kourai