title: Ganoderma tsugae extract inhibits expression of epidermal growth factor receptor and angiogenesis in human epidermoid carcinoma cells: In vitro and in vivo.
abstract: We examined the anti-angiogenic effects of Ganoderma tsugae methanol extract (GTME) on human epidermoid carcinoma A-431 cells. Our data indicate that GTME inhibits the expression of epidermal growth factor receptor (EGFR) and vascular endothelial growth factor (VEGF) in vitro and in vivo, and also inhibits the capillary tube formation of human umbilical vein endothelial cells (HUVECs). We also show that the suppression of VEGF expression by GTME can be restored by treatment with EGF. These results suggest that GTME inhibits VEGF expression via the suppression of EGFR expression, resulting in the downregulation of VEGF secretion from epidermoid carcinoma A-431 cells. These findings reveal a novel role for G. tsugae in inhibiting EGFR and VEGF expression, which are important for tumor angiogenesis and growth. Thus, GTME may provide a potential therapeutic approach for anti-tumor treatment.
authors.affiliation: Graduate Institute of Medical Sciences, Taipei Medical University, Taiwan, ROC.
authors.forename: Shih-Chung
authors.initials: SC
authors.lastname: Hsu
authors.forename: Chien-Chih
authors.initials: CC
authors.lastname: Ou
authors.forename: Tzu-Chao
authors.initials: TC
authors.lastname: Chuang
authors.forename: Jhy-Wei
authors.initials: JW
authors.lastname: Li
authors.forename: Yi-Jen
authors.initials: YJ
authors.lastname: Lee
authors.forename: Vinchi
authors.initials: V
authors.lastname: Wang
authors.forename: Jah-Yao
authors.initials: JY
authors.lastname: Liu
authors.forename: Chin-Shiang
authors.initials: CS
authors.lastname: Chen
authors.forename: Song-Chow
authors.initials: SC
authors.lastname: Lin
authors.forename: Ming-Ching
authors.initials: MC
authors.lastname: Kao