Cell immobilization in gelatin-hydroxyphenylpropionic acid hydrogel fibers.
Gelatin-hydroxyphenylpropionic acid (Gtn-HPA) hydrogels are highly porous and biodegradable materials. Herein we report a fiber spinning method that can produce cell-seeded solid and hollow hydrogel fibers by enzymatically cross-linking Gtn-HPA in solutions flowing within a capillary tube. The cell-immobilized hydrogel fibers, with feature sizes down to 20 microm, are formed as a result of continuous cross-linking of cell-mixed hydrogel precursors in a multiphase laminar flow. This fiber formation process is mild enough to retain the cell viability. The continuous fiber formation, simultaneous cell encapsulation, as well as versatile combination of fiber structures provided by this approach make it a promising and effective technique for the preparation of cell-seeded hydrogel scaffolds and carriers for tissue engineering.
Hu
Min
M
Institute of Bioengineering and Nanotechnology, 31 Biopolis Way, The Nanos, Singapore 138669, Singapore.
Kurisawa
Motoichi
M
Deng
Rensheng
R
Teo
Choon-Meng
CM
Schumacher
Annegret
A
Thong
Ya-Xuan
YX
Wang
Lishan
L
Schumacher
Karl M
KM
Ying
Jackie Y
JY