title: Enhanced cellular uptake of chlorine e6 mediated by  stearic acid-grafted chitosan oligosaccharide micelles.
abstract: Chlorines are attractive compounds for photodynamic therapy because of their high absorption in the red wavelength region. The stearic acid-grafted chitosan oligosaccharide (CSO-SA) micelles have been presented as potential candidates for intracellular drug delivery carrier because of their special structure. In this study, CSO-SA micelles were prepared to encapsulate chlorine e6 (Ce6). The physicochemical properties of synthesized CSO-SA micelles were characterized. The critical micelle concentration (CMC) of CSO-SA with 4.96% amino substituted degree (SD %) was about 36.27 +/- 1.51 microg/mL. The Ce6-loaded CSO-SA micelles were then prepared by a dialysis method, and the properties and drug release profiles of Ce6- loaded CSO-SA micelles (CSO-SA/Ce6) were investigated. The loading of Ce6 in the CSO-SA micelles could reach higher drug encapsulation efficiency (%), which was approximately 100%. The size of CSO-SA/Ce6 decreased after the loading of Ce6. The zeta potential of CSO-SA/Ce6 and the drug release rate decreased with the loading content of drug. After the Ce6 molecules were encapsulated into the micelles of CSO-SA, the cellular uptake percentage of Ce6 was much more than that of the free drug. And the cellular uptake percentage of CSO-SA/Ce6 micelles was increased with the incubation time in a short period.
authors.affiliation: College of Pharmaceutical Science, Zhejiang University, Hangzhou, People's Republic of China. hufq@zju.edu.cn
authors.forename: Fu-Qiang
authors.initials: FQ
authors.lastname: Hu
authors.forename: Xiao-Hong
authors.initials: XH
authors.lastname: Jiang
authors.forename: Xuan
authors.initials: X
authors.lastname: Huang
authors.forename: Xiu-Ling
authors.initials: XL
authors.lastname: Wu
authors.forename: Hong
authors.initials: H
authors.lastname: Yuan
authors.forename: Xiao-Hong
authors.initials: XH
authors.lastname: Wei
authors.forename: Yong-Zhong
authors.initials: YZ
authors.lastname: Du