Glucose-sensitive microcapsules from glutaraldehyde cross-linked hemoglobin and glucose oxidase.
We have fabricated the glucose-sensitive protein hollow microcapsules from hemoglobin (Hb) and glucose oxidase (GOx) via layer-by-layer technique with glutaraldehyde (GA) as a cross-linking agent. We demonstrated the coupled enzymatic reactions between Hb and GOx within capsules by means of CLSM. The real-time monitoring of the consumption of glucose may be useful for the design of a glucose sensor. Furthermore, to further study the effect of glucose on the capsules, we investigated the wall permeability of (Hb/GOx)(5) capsules. It was found that the glucose consumption could enhance the permeability of (Hb/GOx)(5) capsules. This is significant for the development of advanced drug delivery devices. Therefore, the fabricated Hb/GOx capsules may be a potential candidate for the creation of multifunctional devices.
Qi
Wei
W
Beijing National Laboratory for Molecular Science, International Joint Laboratory, CAS Key Laboratory of Colloid and Interface Science, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, China.
Yan
Xuehai
X
Duan
Li
L
Cui
Yue
Y
Yang
Yang
Y
Li
Junbai
J