Infectious RNA transcripts derived from cloned cDNA of a pepino mosaic virus isolate.
For the first time, a full-length cDNA clone of the RNA genome of pepino mosaic virus (PepMV) was constructed. RNA was extracted from purified virions of isolate PepMV-Pa and used for cDNA synthesis. The full-length cDNA was produced as one 6.4-kb fragment representing the entire PepMV genome. This fragment was ligated into the pCR-XL-TOPO vector downstream of T7 RNA polymerase promoter, which was included in the 5' primer sequence used for RT-PCR. The PepMV-Pa RNA transcripts obtained were infectious in different host plants, causing symptoms indistinguishable from those of the wild-type isolate. The presence and authenticity of the progeny virus were verified by ELISA, RT-PCR and nucleotide sequencing.
Hasiów-Jaroszewska
Beata
B
Department of Virology and Bacteriology, Institute of Plant Protection, National Research Institute, ul. Wegorka 20, 60-318 Poznan, Poland. B.Hasiow@ior.poznan.pl
Borodynko
Natasza
N
Pospieszny
Henryk
H