Effect of ovariectomy on the gene expression of detrusor muscarinic receptors in female rats.
Eight rats served as control subjects with sham surgeries and eight rats underwent ovariectomy. Two months later, the expression of muscarinic receptors, neuronal nitric oxide synthase (nNOS), and estrogen receptor (R) at the mRNA level were assessed by reverse-transcription polymerase chain reaction. The results showed that performing an ovariectomy in a virgin rat appears to have little effect on the gene expression of detrusor muscarinic receptors and urethral nNOS despite the menopausal events that occurred.
Long
Cheng-Yu
CY
Graduate Institute of Medicine, Center of Excellence for Environmental Medicine, Kaohsiung, Taiwan; Department of Obstetrics and Gynecology, Kaohsiung Municipal Hsiao-Kang Hospital, Kaohsiung, Taiwan.
Hsu
Chun-Shuo
CS
Department of Obstetrics and Gynecology, Buddhist Dalin Tzu Chi General Hospital, Chiayi, Taiwan.
Shao
Pei-Lin
PL
Graduate Institute of Medicine, Center of Excellence for Environmental Medicine, Kaohsiung, Taiwan.
Liu
Cheng-Min
CM
Graduate Institute of Medicine, Center of Excellence for Environmental Medicine, Kaohsiung, Taiwan.
Su
Juin-Huang
JH
Graduate Institute of Medicine, Center of Excellence for Environmental Medicine, Kaohsiung, Taiwan; Department of Obstetrics and Gynecology, Kaohsiung Medical University, Kaohsiung, Taiwan.
Tsai
Eing-Mei
EM
Graduate Institute of Medicine, Center of Excellence for Environmental Medicine, Kaohsiung, Taiwan; Department of Obstetrics and Gynecology, Kaohsiung Medical University, Kaohsiung, Taiwan. Electronic address: K83263@kmhk.kmu.edu.tw.