Fusion protein encoded by a CTLA-4 targeted DNA construct binds to human dendritic cells.
Fusing antigens to cytotoxic T-lymphocyte antigen 4 (CTLA-4) represents an effective approach to enhance DNA vaccine efficacy. It has been speculated that the direct targeting of CTLA-4 fusion antigens to antigen-presenting cells (APCs) causes antigens to be processed and presented to T cells more efficiently, leading to a stronger immune response. In the present study, dendritic cells (DCs), the most potent APCs, were generated from human monocytes. The specific binding of CTLA-4 fusion protein to DCs was investigated by flow cytometry. The results showed that the CTLA-4 fusion protein was capable of binding to the B7 molecules on human DCs with specificity.
Xu
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Key Laboratory for Oral Biomedical Engineering of Ministry of Education, School and Hospital of Stomatology, Wuhan University, Wuhan, Hubei, China.
Zhang
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Fan
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Liu
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Li
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Yu
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Tian
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