title: Collagen antibody-induced arthritis in mice: development of a new arthritogenic 5-clone cocktail of monoclonal anti-type II collagen antibodies.
abstract: A cocktail of 4 monoclonal anti-type II collagen antibodies recognizing conserved epitopes located within the CB11 fragment (CII 124-402) of type II collagen is currently used as an arthritogenic antibody preparation for inducing collagen antibody induced arthritis (CAIA). In order to increase the arthritogenicity of this cocktail, we have developed 7 new monoclonal antibodies to anti-type II collagen from spleen cells of DBA/1J mice immunized with bovine type II collagen, and tested for their additional effect on the arthritogenicity over that of the current 4-clone cocktail. Three of the clones (CII-3, -5 and -6) bind to the LyC1 (CII 124-290) peptide of CB11 and 1 (CII-7) of the clones binds to CB9.7 (CII 898-1020), and highly cross-reacted with other species of type II collagen. This indicates that these clones recognize conserved epitopes within type II collagen, including mouse type II collagen. On the other hand, 2 other clones (CII-1 and -4) directed against CB9.7 and 1 clone (CII-2) against CB8 (CII 403-551) were less reactive with other species of type II collagen. The arthritogenicity of the current 4-clone cocktail was significantly increased by addition of a fifth clone, CII-3. No effects were observed with other clones. The arthritogenicity of this new 5-clone cocktail was 2-fold greater than the current 4-clone cocktail in all strains of mice tested: the CIA-responder strain DBA/1J, the CIA-resistant BALB/c (H-2(d)), the T-cell deficient C.B-17/l scid/scid and the CAIA-low responder C57BL/6 (H-2(b)) strain. These results clearly indicated the importance of epitope specificity of arthritogenicity of autoantibodies to type II collagen. Due to its enhanced arthritogenicity, this 5-clone cocktail is capable of inducing a more consistent and severe arthritis with lower doses compared to the current 4-clone cocktail, and will provide an effective new reagent for inducing arthritis in various strains of CAIA low responder mice.
authors.affiliation: Department of Pharmacology, Kobe Pharmaceutical University, Kobe-shi, Hyogo-ken, Japan.
authors.forename: Pilaiwanwadee
authors.initials: P
authors.lastname: Hutamekalin
authors.forename: Takayuki
authors.initials: T
authors.lastname: Saito
authors.forename: Kouya
authors.initials: K
authors.lastname: Yamaki
authors.forename: Nobuaki
authors.initials: N
authors.lastname: Mizutani
authors.forename: David D
authors.initials: DD
authors.lastname: Brand
authors.forename: Takaki
authors.initials: T
authors.lastname: Waritani
authors.forename: Kuniaki
authors.initials: K
authors.lastname: Terato
authors.forename: Shin
authors.initials: S
authors.lastname: Yoshino