title: Adaptive immune response in JAM-C-deficient mice: normal initiation but reduced IgG memory.
abstract: We have recently shown that junctional adhesion molecule (JAM)-C-deficient mice have leukocytic pulmonary infiltrates, disturbed neutrophil homeostasis, and increased postnatal mortality. This phenotype was partially rescued when mice were housed in ventilated isolators, suggesting an inability to cope with opportunistic infections. In the present study, we further examined the adaptive immune responses in JAM-C(-/-) mice. We found that murine conventional dendritic cells express in addition to Mac-1 and CD11c also JAM-B as ligand for JAM-C. By in vitro adhesion assay, we show that murine DCs can interact with recombinant JAM-C via Mac-1. However, this interaction does not seem to be necessary for dendritic cell migration and function in vivo, even though JAM-C is highly expressed by lymphatic sinuses of lymph nodes. Nevertheless, upon immunization and boosting with a protein Ag, JAM-C-deficient mice showed decreased persistence of specific circulating Abs although the initial response was normal. Such a phenotype has also been observed in a model of Ag-induced arthritis, showing that specific IgG2a Ab titers are reduced in the serum of JAM-C(-/-) compared with wild-type mice. Taken together, these data suggest that JAM-C deficiency affects the adaptive humoral immune response against pathogens, in addition to the innate immune system.
authors.affiliation: Department of Pathology and Immunology, University of Geneva School of Medicine, Geneva, Switzerland.
authors.forename: Claudia
authors.initials: C
authors.lastname: Zimmerli
authors.forename: Boris P L
authors.initials: BP
authors.lastname: Lee
authors.forename: Gaby
authors.initials: G
authors.lastname: Palmer
authors.forename: Cem
authors.initials: C
authors.lastname: Gabay
authors.forename: Ralf
authors.initials: R
authors.lastname: Adams
authors.forename: Michel
authors.initials: M
authors.lastname: Aurrand-Lions
authors.forename: Beat A
authors.initials: BA
authors.lastname: Imhof