title: Chemical Composition and Antimicrobial Properties of Piper ovatum Vahl.
abstract: The chemical composition of the essential oil obtained from the leaves of Piperovatum Vahl by hydrodistillation was analyzed by GC-MS. The main constituents found were delta-amorphene (16.5 %), cis-muurola-4(14),5-diene (14.29 %) and gamma-muurolene(13.26%). The crude extracts and isolated compounds were screened for their antimicrobial activity. Hydroalcoholic extracts of different parts of Piper ovatum Vahl, essential oil andamides isolated from leaves were tested against Gram-positive and Gram-negative bacteria and Candida species. All extracts and amides were active against Bacillus subtilis andCandida tropicalis, including clinical strains. Essential oil was active against C. tropicalis.These amides showed an inhibitory effect on the adherence of C. tropicalis ATCC 28707 on cover glasses at 10 microg/mL, but did not show morphological alterations at the tested concentrations. Amides were identified as piperovatine and piperlonguminine, and showed MIC values of 15.6 and 31.2 microg/mL to B. subtilis and 3.9 microg/mL to C. tropicalis, and low toxic effects to Vero cells and macrophages.
authors.affiliation: Programa de Pós-graduação em Ciências Farmacêuticas, Universidade Estadual de Maringá, Maringá, Paraná, Brazil.
authors.forename: Daniel Rodrigues
authors.initials: DR
authors.lastname: Silva
authors.forename: Eliana Harue
authors.initials: EH
authors.lastname: Endo
authors.forename: Benedito Prado Dias
authors.initials: BP
authors.lastname: Filho
authors.forename: Celso Vataru
authors.initials: CV
authors.lastname: Nakamura
authors.forename: Terezinha Inez Estivaleti
authors.initials: TI
authors.lastname: Svidzinski
authors.forename: Amanda
authors.initials: A
authors.lastname: de Souza
authors.forename: Maria Claudia M
authors.initials: MC
authors.lastname: Young
authors.forename: Tânia
authors.initials: T
authors.lastname: Ueda-Nakamura
authors.forename: Diógenes Aparício Garcia
authors.initials: DA
authors.lastname: Cortez