title: Transcriptomic microarray analysis of corrinoid responsive genes in Dehalococcoides ethenogenes strain 195.
abstract: Dehalococcoides ethenogenes strain 195 utilizes corrinoid-containing reductive dehalogenases to reduce the environmental pollutants tetrachloroethene and trichloroethene to ethene. Although corrinoids are essential for dehalogenation activity, strain 195 cannot biosynthesize corrinoids de novo. To improve our understanding of corrinoid physiology in this bacterium, whole-genome microarrays were applied to characterize the transcriptome during growth with excess and limiting concentrations of the corrinoid cyanocobalamin. Additional studies examined the effects of exposure to spent medium from a methanogenic chloroethene-dehalogenating enrichment culture (designated ANAS). Both excess cyanocobalamin and ANAS spent medium resulted in the downregulation of two genes (DET0125-0126) that are encoded downstream of a putative cobalamin riboswitch. In contrast, only ANAS spent medium resulted in the downregulation of three duplicated genes (DET0657-0659/DET0691-0693) encoded downstream of a second putative cobalamin riboswitch. These latter genes are predicted to be involved in synthesizing the lower ligand base that is attached to cobyric acid. It is also notable that only excess cyanocobalamin resulted in the downregulation of a predicted cobalamin transport system. Together, these results imply that ANAS spent medium contains corrinoid forms different from cyanocobalamin and that strain 195 adjusts its metabolism according to the corrinoid forms available for uptake.
authors.forename: David R
authors.initials: DR
authors.lastname: Johnson
authors.forename: Audra
authors.initials: A
authors.lastname: Nemir
authors.forename: Gary L
authors.initials: GL
authors.lastname: Andersen
authors.forename: Stephen H
authors.initials: SH
authors.lastname: Zinder
authors.forename: Lisa
authors.initials: L
authors.lastname: Alvarez-Cohen