title: Application of loop-mediated isothermal amplification for detection of Yersinia enterocolitica in pork meat.
abstract: A loop-mediated isothermal amplification (LAMP) method was developed for the detection of Yersinia enterocolitica isolates in both pure bacterial cultures and pork meat. The LAMP primers, which corresponded to the gyrB gene, accurately identified 4 different bioserotypes of Y. enterocolitica. These primers failed to detect Y. pseudotuberculosis, Y. frederiksenii, and 17 non-Yersinia strains. The sensitivity of the LAMP assay for the detection of Y. enterocolitica in pure culture was 65 CFU/mL (31.6 fg of genomic DNA). The LAMP assay was conducted for the detection of Y. enterocolitica strains in 21 pig tonsil samples and 73 pork meat samples obtained from 94 slaughtered pigs belonging to 4 different herds. Y. enterocolitica was found to be present in 4 tonsil samples and none in meat samples. This is the first report in which the LAMP assay was employed for the detection of Y. enterocolitica in food samples.
authors.affiliation: Shandong Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China, Qingdao, China. ghw75@126.com
authors.forename: Hongwei
authors.initials: H
authors.lastname: Gao
authors.forename: Zhiwen
authors.initials: Z
authors.lastname: Lei
authors.forename: Juntao
authors.initials: J
authors.lastname: Jia
authors.forename: Shufeng
authors.initials: S
authors.lastname: Wang
authors.forename: Ying
authors.initials: Y
authors.lastname: Chen
authors.forename: Min
authors.initials: M
authors.lastname: Sun
authors.forename: Chengzhu
authors.initials: C
authors.lastname: Liang