title: Patterned growth and differentiation of human cord blood-derived neural stem cells on bio-functionalized surfaces.
abstract: Bio-functionalized surfaces were prepared to study the adherence and differentiation capacity of neural stem cells derived from human umbilical cord blood (HUCB-NSC). Cell growth platforms containing arranged arrays of adhesive molecules were created by microcontact printing on a biologically inert surface. Biomolecules used to prepare microarray platforms included the extracellular matrix protein fibronectin and the polyaminoacid poly-L-lysine. HUCB-NSC plated on microplatforms at various serum conditions showed serum and molecule type dependent capacity for adhesion and differentiation. Poly-L-lysine allowed the maintenance of stem-like non differentiated cells attached to the surface, whereas fibronectin promoted spreading and neural commitment. Serum deprivation did not influence the attachment of HUCB-NSC to fibronectin, but significantly enhanced the attachment to poly-L-lysine and promoted dBcAMP induced neuronal differentiation. A bio-pattern of squares with interconnecting lines was used to guide neuronal differentiation by directing cell protrusion outgrowth. Tailoring the geometry of the bio-pattern enabled directing and monitoring of the neural stem cells. development in the large scale multiparameter biotests.
authors.affiliation: In Vitro Toxicology/ECVAM, Institute for Health and Consumer Protection, European Commission, Joint Research Centre, Ispra, Italy. buzanska@cmdik.pan.pl.
authors.forename: Leonora
authors.initials: L
authors.lastname: Buzanska
authors.forename: Ana
authors.initials: A
authors.lastname: Ruiz
authors.forename: Marzena
authors.initials: M
authors.lastname: Zychowicz
authors.forename: Hubert
authors.initials: H
authors.lastname: Rauscher
authors.forename: Laura
authors.initials: L
authors.lastname: Ceriotti
authors.forename: Francois
authors.initials: F
authors.lastname: Rossi
authors.forename: Pascal
authors.initials: P
authors.lastname: Colpo
authors.forename: Krystyna
authors.initials: K
authors.lastname: Domańska-Janik
authors.forename: Sandra
authors.initials: S
authors.lastname: Coecke