title: Cellulophaga tyrosinoxydans sp. nov., a tyrosinase-producing bacterium isolated from seawater.
abstract: An aerobic, gliding, yellow-pigmented bacterium lacking flagella and showing strong tyrosinase activity, designated strain EM41(T), was isolated from seawater on the eastern coast of Jeju Island in Korea. Growth was observed at 15-35 degrees C (optimum, 25-30 degrees C) and at pH 6.5-9.0 (optimum, pH 7.0-8.5). Cells were Gram-negative, negative for flexirubin pigments and catalase- and oxidase-positive. The G+C content of the genomic DNA was 33.5 mol% and the major respiratory quinone was menaquinone-6 (MK-6). Comparative 16S rRNA gene sequence analysis showed that strain EM41(T) formed a distinct phyletic line within the genus Cellulophaga with a 100 % bootstrap value and was most closely related to Cellulophaga pacifica KMM 3664(T) (97.0 % sequence similarity). The level of DNA-DNA relatedness between strain EM41(T) and C. pacifica KMM 3664(T) was about 17.8 %. On the basis of phenotypic and genotypic data, strain EM41(T) is considered to represent a novel species of the genus Cellulophaga, for which the name Cellulophaga tyrosinoxydans sp. nov. is proposed. The type strain is EM41(T) (=KCTC 22297(T)=DSM 21164(T)).
authors.affiliation: Department of Environmental Education, Sunchon National University, Sunchon 540-742, Republic of Korea.
authors.forename: Hyung-Yeel
authors.initials: HY
authors.lastname: Kahng
authors.forename: Bok Sil
authors.initials: BS
authors.lastname: Chung
authors.forename: Dong-Heon
authors.initials: DH
authors.lastname: Lee
authors.forename: Jae Sung
authors.initials: JS
authors.lastname: Jung
authors.forename: Jin Ho
authors.initials: JH
authors.lastname: Park
authors.forename: Che Ok
authors.initials: CO
authors.lastname: Jeon