title: Assessment study about using underground water for tilapia culture for the first time in El-Bahria Oasis Desert, Egypt.
abstract: The present study was carried out in El-Bahria Oasis desert (Giza-Egypt) for 8 months (March-October) as a new study to assess the culture of tilapia spp. in underground well water. The obtained results showed a significant increase (P<0.01) in the values of plasma total protein, glucose, AST (aspartate aminotransferase), ALT (alanine aminotransferase), creatinine, uric acid, sodium, potassium and magnesium as well as a significant decrease (P<0.01) in plasma total lipids and calcium concentration. Furthermore, iron content in some selected vital organs was increased gradually with time, the studied organs are arranged according to their iron content in the following order: spleen > liver > kidney > gills > muscle. Remarkable changes were observed in the chemical muscle composition where the results showed a significant increase (P<0.01) in muscle water content, total lipids and ash. However, a significant decrease (P<0.01) in muscle total protein at the end of the study was observed. The growth of all male farmed tilapia in well water with a 3.2 mg/l iron concentration was unexpected; despite the presence of this high concentration of iron, the weight gain of cultured fish was 250 +/- 14.5 g. Molecular techniques are used nowadays as a good indicator for assessing the alteration in the genomes. RAPD-PCR technique indicated appearance of some changes in polymorphism band patterns. There also exists a distinct distance between the band patterns of cultured fish (T) and control fish (C). Histopathlogical sections showed pathological alterations in liver, kidney gills and spleen and the obtained results were discussed.
authors.affiliation: Hydrobiology Department, National Research Centre, Dokki, Gizza, Egypt. h3abbas@yahoo.com
authors.forename: Hossam H
authors.initials: HH
authors.lastname: Abbas
authors.forename: Fagr Kh
authors.initials: FKh
authors.lastname: Ali
authors.forename: Amany M
authors.initials: AM
authors.lastname: Kenawy