Structural understanding of quorum-sensing inhibitors by molecular modeling study in Pseudomonas aeruginosa.
Inhibitors of 3OC12, an initial signal molecule of the quorum sensing (QS) signaling cascade in Pseudomonas aeruginosa have been developed. Eight inhibitor candidates were synthesized by substituting the head part of 3-oxododecanoyl-homoserine lactone (3OC12) with different aromatic rings, and their docking poses and scores (binding energies) were predicted by in silico modeling study. All compounds gave better docking scores than 3OC12 and good inhibition effects on LasR activity in the in vivo bioassay. Like the modifications in the tail part of 3OC12 in our previous study Kim et al. (2008), the head-part modifications also showed inhibition activity in a fairly good proportion to the docking scores from the modeling analysis. This implies that the head part of 3OC12 also contributes significantly to forming the active conformation of the LasR-3OC12 complex, and its modification could effectively induce the inactive conformation of the complex. We suggest that the head part of 3OC12 is also a good target moiety to develop the structure-based Pseudomonas QS inhibitors.
Kim
Cheoljin
C
Departments of Chemistry, Princeton University, NJ 08544, USA.
Kim
Jaeeun
J
Park
Hyung-Yeon
HY
Lee
Joon-Hee
JH
Park
Hee-Jin
HJ
Kim
Chan Kyung
CK
Yoon
Jeyong
J