title: Expanding the scope of protein trans-splicing to fragment ligation of an integral membrane protein: towards modulation of porin-based ion channels by chemical modification.
abstract: It's raining, it's porin: Fragment ligation of OmpF ion channels was achieved by using the split Psp-GBD Pol intein; this allowed reconstitution of active trimeric porin. In combination with cysteine modification at an internal position, the porin's conductance properties were altered.
authors.affiliation: Technische Universität Dortmund, Fakultät Chemie-Chemische Biologie, Otto-Hahn-Strasse, Dortmund, Germany.
authors.forename: Steffen
authors.initials: S
authors.lastname: Brenzel
authors.forename: Menekse
authors.initials: M
authors.lastname: Cebi
authors.forename: Philipp
authors.initials: P
authors.lastname: Reiss
authors.forename: Ulrich
authors.initials: U
authors.lastname: Koert
authors.forename: Henning D
authors.initials: HD
authors.lastname: Mootz