title: Structure of Debaryomyces castellii CBS 2923 phytase.
abstract: Phytate (myo-inositol hexakisphosphate) is the primary storage form of phosphate in seeds and legumes (Reddy et al., 1982). Phytases are phosphatases that hydrolyze phytate to less phosphorylated myo-inositol derivatives and inorganic phosphate. The crystal structure of phytase from Debaryomyces castellii has been determined at 2.3 A resolution. The crystals belonged to space group P6(5)22, with unit-cell parameters a = 121.65, c = 332.24 A. The structure was solved by molecular replacement and refined to a final R factor of 15.7% (R(free) = 20.9%). The final model consists of a dimer (with two monomers of 458 residues), five NAG molecules and 628 water molecules.
authors.affiliation: UMR IR2B, Equipe Génie Microbiologique et Enzymatique, ENSAM-INRA, Montpellier, France.
authors.forename: M
authors.initials: M
authors.lastname: Ragon
authors.forename: F
authors.initials: F
authors.lastname: Hoh
authors.forename: A
authors.initials: A
authors.lastname: Aumelas
authors.forename: L
authors.initials: L
authors.lastname: Chiche
authors.forename: G
authors.initials: G
authors.lastname: Moulin
authors.forename: H
authors.initials: H
authors.lastname: Boze