title: Cadmium biosorption by Streptomyces sp. F4 isolated from former uranium mine.
abstract: 46 actinomycetes were isolated from two polluted sites and one unpolluted site. One strain, F4, was selected through primary qualitative screening assays because of its cadmium resistance, and physiologically and taxonomically characterized. F4 was able to grow at 7.5% NaCl and 100 microg/ml lysozyme and at a pH between 6 and 10. 16S rDNA sequence analysis showed that F4 was closely related to Streptomyces tendae. Growth of Streptomyces sp. F4 on culture medium with 8 mg/l Cd(2+) for 8 days showed 80% inhibition. Maximum specific biosorption was 41.7 mg Cd(2+)/g dry weight after 7 days of growth and highest Cd(2+ )concentration was found in the cell wall (41.2%). The exopolysaccharide layer only contained 7.4%, whereas 39.4% of Cd(2+) was found in the cytosolic fraction. Twelve % was found in the ribosomes and membrane fraction. This was verified with TEM, showing Streptomyces sp. F4 cytoplasm with dark granulate appearance. This study could present the potential capacity of Streptomyces sp. F4 for Cd(2+) bioremediation.
authors.affiliation: Planta Piloto de Procesos Industriales y Microbiológicos, CONICET. Av Belgrano y Pasaje Caseros, Tucumán, Argentina.
authors.forename: Manuel Louis
authors.initials: ML
authors.lastname: Siñeriz
authors.forename: Erika
authors.initials: E
authors.lastname: Kothe
authors.forename: Carlos Mauricio
authors.initials: CM
authors.lastname: Abate