title: Association of sugar content QTL and PQL with physiological traits relevant to frost damage resistance in pea under field and controlled conditions.
abstract: To increase yield in pea (Pisum sativum L.), autumn sowing would be preferable. Hence, frost tolerance of pea became a major trait of interest for breeders. In order to better understand the cold acclimation in pea, Champagne a frost tolerant line and Terese, a frost sensitive line, and their recombinant inbred lines (RIL) were studied. RIL frost tolerance was evaluated by a frost damage scale under field as well as controlled conditions. A quantitative trait loci (QTL) approach was used to identify chromosomal regions linked to frost tolerance. The detected QTL explained from 6.5 to 46.5% of the phenotypic variance. Amongst them, those located on linkage groups 5 and 6 were consistent with over all experiments, in field as well as in controlled environments. In order to improve the understanding of the frost tolerance mechanisms, several cold acclimation key characters such as concentration of sugars, electrolyte leakage, osmotic pressure, and activity of RuBisCO were assessed. Some of these physiological QTL colocalised with QTL for frost damage, in particular two raffinose QTL on LG5 and LG6 and one RuBisCO activity QTL on LG6, explaining 8.8 to 27.0% of the phenotypic variance. In addition, protein quantitative loci were mapped; some of them colocalised with frost damage and physiological QTL on LG5 and LG6, explaining 16.0-43.6% of the phenotypic variance. Raffinose metabolism and RuBisCO activity and its effect on photosynthesis might play a major role in cold acclimation of pea.
authors.affiliation: SADV Laboratoire des Stress Abiotiques et Différenciation des Végétaux cultivés bât SN2, 3ème étage, UMR USTL-INRA 1281, Université des Sciences et Technologies de Lille1, 59655, Villeneuve d'Ascq, France.
authors.forename: Estelle
authors.initials: E
authors.lastname: Dumont
authors.forename: Véronique
authors.initials: V
authors.lastname: Fontaine
authors.forename: Christophe
authors.initials: C
authors.lastname: Vuylsteker
authors.forename: Hélène
authors.initials: H
authors.lastname: Sellier
authors.forename: Sylvie
authors.initials: S
authors.lastname: Bodèle
authors.forename: Najia
authors.initials: N
authors.lastname: Voedts
authors.forename: Rosemonde
authors.initials: R
authors.lastname: Devaux
authors.forename: Marlène
authors.initials: M
authors.lastname: Frise
authors.forename: Komlan
authors.initials: K
authors.lastname: Avia
authors.forename: Jean-Louis
authors.initials: JL
authors.lastname: Hilbert
authors.forename: Nasser
authors.initials: N
authors.lastname: Bahrman
authors.forename: Eric
authors.initials: E
authors.lastname: Hanocq
authors.forename: Isabelle
authors.initials: I
authors.lastname: Lejeune-Hénaut
authors.forename: Bruno
authors.initials: B
authors.lastname: Delbreil