Replication-defective adenovirus vectors with multiple deletions do not induce measurable vector-specific T cells in human trials.
The magnitude and character of adenovirus serotype 5 (Ad5)-specific T cells were determined in volunteers with and without preexisting neutralizing antibodies (NAs) to Ad5 who received replication-defective Ad5 (rAd5)-based human immunodeficiency virus vaccines. There was no correlation between T-cell responses and NAs to Ad5. There was no increase in magnitude or activation state of Ad5-specific CD4(+) T cells at time points where antibodies to Ad5 and T-cell responses to the recombinant gene products could be measured. These data indicate that rAd5-based vaccines containing deletions in the E1, E3, and E4 regions do not induce appreciable expansion of vector-specific CD4(+) T cells.
Koup
Richard A
RA
Immunology Laboratory, Immunology Core Section, Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA. rkoup@mail.nih.gov
Lamoreaux
Laurie
L
Zarkowsky
David
D
Bailer
Robert T
RT
King
C Richter
CR
Gall
Jason G D
JG
Brough
Douglas E
DE
Graham
Barney S
BS
Roederer
Mario
M