title: Combinations of DEAD box proteins distinguish distinct types of RNA:  protein complexes in neurons.
abstract: Transport of mRNAs to axons and dendrites in neurons is important for growth, polarization and plasticity. Recent proteomic studies in neurons have identified a number of DEAD box proteins as components of RNA granules. Using DEAD box proteins as markers, we have defined classes of RNA:protein structures present in neurons. In particular, we demonstrate that the conjunction of DEAD box 1 and DEAD box 3 identifies a motile ribosome-containing RNA granule present in both axons and dendrites that is similar to the biochemically isolated RNA granule. Conjunction of DEAD box 1 and the novel protein CGI-99 defines a distinct complex in neurons. Attempts to define a P-body like structure with expression of DEAD box 6 and decapping enzymes suggest that this structure may be more complex in neuronal processes than in other compartments. These studies hint at a great complexity in RNA transport and storage in neuronal processes.
authors.affiliation: Department of Neurology and Neurosurgery, McGill University, Montreal Neurological Institute, BT 110, 3801 University Street, Montreal, Quebec H3A2B4, Canada.
authors.forename: Linda C
authors.initials: LC
authors.lastname: Miller
authors.forename: Vanessa
authors.initials: V
authors.lastname: Blandford
authors.forename: Robyn
authors.initials: R
authors.lastname: McAdam
authors.forename: Maria R
authors.initials: MR
authors.lastname: Sanchez-Carbente
authors.forename: Frederique
authors.initials: F
authors.lastname: Badeaux
authors.forename: Luc
authors.initials: L
authors.lastname: DesGroseillers
authors.forename: Wayne S
authors.initials: WS
authors.lastname: Sossin