title: Distribution of MICA diversity in the Chinese Han population by polymerase chain reaction sequence-based typing for exons 2-6.
abstract: Major histocompatibility complex class I chain-related gene A (MICA) is located 46 kb centromeric to HLA-B locus and encodes a stress-inducible protein. MICA allelic variation is thought to be associated with disease susceptibility and immune response to transplants. In this study, polymerase chain reaction sequence-based typing (PCR-SBT) method for MICA alleles has been established. Genomic DNAs from 100 healthy Chinese Han individuals were typed for MICA alleles by this method. The microsatellite polymorphism in the exon 5 of MICA gene, MICA*Del allele, and human leukocyte antigen-B alleles was also detected by the polymerase chain reaction-GeneScan, polymerase chain reaction sequence-specific primer, and PCR-SBT methods, respectively. Fourteen MICA alleles were found in the population, with MICA*00801/04 having the highest frequency of 27.0%. MICA*A4, *A5, *A5.1, *A6, and *A9 microsatellites were identified. Two samples with HLA-B*4801 were MICA*Del positive, with the frequency of 1.0%. The data showed that the new PCR-SBT method for MICA alleles was reliable and Chinese Han population was distinct in distribution of MICA alleles.
authors.affiliation: HLA Typing Laboratory, Blood Center of Zhejiang Province, Hangzhou, Zhejiang Province, China.
authors.forename: F
authors.initials: F
authors.lastname: Zhu
authors.forename: H
authors.initials: H
authors.lastname: Zhao
authors.forename: Y
authors.initials: Y
authors.lastname: He
authors.forename: W
authors.initials: W
authors.lastname: Zhang
authors.forename: J
authors.initials: J
authors.lastname: He
authors.forename: X
authors.initials: X
authors.lastname: Xu
authors.forename: L
authors.initials: L
authors.lastname: Yan