title: Anti-CD25 antibodies decrease the ability of human dendritic cells to prime allogeneic CD4 T cells.
abstract: Anti-CD25 monoclonal antibodies are largely used in clinical transplantation to prevent acute allograft rejection episodes. Although their effects on T lymphocytes have been extensively studied, their impact on human dendritic cells (DCs) has been less reported. Furthermore, the role of the interleukin-2 in DC functions has not yet been fully elucidated. In this study, we observed that stimulation of human monocyte-derived DCs with lipopolysa ccharide or CD40L strongly induced the expression of CD25. We showed that pretreatment of DC with anti-CD25 diminished their ability to prime T-helper cells. In contrast, humanized anti-CD25 monoclonal antibodies did not affect the up-regulation of CD86, CD80, CD83, HLA-DR, or CD40 induced by lipopolysaccharide stimulation. This study supported previously unrecognized effects of anti-CD25 monoclonal antibodies on DCs that may contribute to their clinical efficacy.
authors.affiliation: JE 2448, Faculté de Médecine, Université François Rabelais, Tours, France.
authors.forename: K
authors.initials: K
authors.lastname: Mnasria
authors.forename: C
authors.initials: C
authors.lastname: Lagaraine
authors.forename: F
authors.initials: F
authors.lastname: Velge-Roussel
authors.forename: Y
authors.initials: Y
authors.lastname: Lebranchu
authors.forename: C
authors.initials: C
authors.lastname: Baron