Click chemistry for the identification of G-quadruplex structures: discovery of a DNA-RNA G-quadruplex.
A trap that closes with a "click": The copper-catalyzed azide-alkyne cycloaddition can occur in different G-quadruplex structures (see scheme). The species trapped by the click reaction can then be separated and analyzed. By using this approach, a DNA-RNA hybrid-type G-quadruplex structure formed by human telomeric DNA and RNA sequences was detected.
Xu
Yan
Y
Research Center for Advanced Science and Technology, The University of Tokyo, Komaba, Meguro-ku, Tokyo 153-8904, Japan. xuyan@mkomi.rcast.u-tokyo.ac.jp
Suzuki
Yuta
Y
Komiyama
Makoto
M