title: Emergence of cutaneous leishmaniasis in a border area at south-east of Iran: an epidemiological survey.
abstract: OBJECTIVE
  Cutaneous leishmaniasis (CL) has been recently emerged in new foci, posing a public health problem. Increasing cases of CL have been reported during recent years from a border area between Iran and Pakistan, a previously non-endemic area. The present study was designed for epidemiological and parasitological characterization of the disease for the first time in this area.
  
  
  METHODS
  A total of 3100 individuals from the city of Mirjaveh and its four rural districts were randomly selected and surveyed from March 2005 to February 2006. Microscopic examination, in vitro culture, mouse inoculations and species-specific kDNA-PCR assay were carried out for Leishmania detection and species identification.
  
  
  RESULTS
  CL was endemic in an important rural district of Mirjaveh, presenting active lesions and scars in 6.6 and 9.5%, respectively. The highest rates of both active lesions and scars were found in the age group of 10 years or under with significant differences (p < 0.05) comparing to the older age groups. No association between genders and the rate of leishmaniasis was observed (p > 0.05). The most affected location was upper limb, 39.2% of ulcers and 41.7% of scars. Inoculation of the clinical isolates on Balb/c mice, led to the development of ulcers in the animals, implying that the causative parasite is Leishmania major. The PCR amplification also generated amplicons specific to L. major.
  
  
  CONCLUSIONS
  It can be concluded that Mirjaveh is an endemic region of cutaneous leishmaniasis as a new focus due to the recent emergence in this border area of south-east of Iran with a major contribution of L. major, as the causative parasite species.
authors.affiliation: Zahedan Research Centre for Infectious Diseases and Tropical Medicine, Zahedan University of Medical Sciences, Iran. afparm1@yahoo.co.uk
authors.forename: A
authors.initials: A
authors.lastname: Fazaeli
authors.forename: B
authors.initials: B
authors.lastname: Fouladi
authors.forename: I
authors.initials: I
authors.lastname: Sharifi