title: Development of a sensitive and selective LC/MS/MS method for the simultaneous determination of intracellular 1-beta-D-arabinofuranosylcytosine triphosphate (araCTP), cytidine triphosphate (CTP) and deoxycytidine triphosphate (dCTP) in a human follicular lymphoma cell line.
abstract: A method was developed for the quantification of araCTP, CTP and dCTP in a human follicular lymphoma cell line. This method involves solid phase extraction (SPE) using a weak anion-exchanger (WAX) cartridge, a porous graphitic carbon high-performance liquid chromatography (HPLC) column separation, and tandem mass spectrometry (MS/MS) detection. By using a triple quadrupole mass spectrometer operating in negative ion multiple reaction monitoring (MRM) mode, the method was able to achieve a lower limit of quantification (LLOQ) of 0.1 microg mL(-1) for araCTP and of 0.01 microg mL(-1) for both CTP and dCTP. The method was validated and used to determine the amount of araCTP, CTP and dCTP formed after incubation of araC and an araCMP prodrug in the human follicular lymphoma cell line RL.
authors.affiliation: Institut des Biomolécules Max Mousseron, UMR 5247 CNRS-UM1-UM2, Université Montpellier 2, Montpellier Cedex 05, France.
authors.forename: Céline
authors.initials: C
authors.lastname: Crauste
authors.forename: Isabelle
authors.initials: I
authors.lastname: Lefebvre
authors.forename: Michael
authors.initials: M
authors.lastname: Hovaneissian
authors.forename: Jean Yves
authors.initials: JY
authors.lastname: Puy
authors.forename: Béatrice
authors.initials: B
authors.lastname: Roy
authors.forename: Suzanne
authors.initials: S
authors.lastname: Peyrottes
authors.forename: Sabine
authors.initials: S
authors.lastname: Cohen
authors.forename: Jérôme
authors.initials: J
authors.lastname: Guitton
authors.forename: Charles
authors.initials: C
authors.lastname: Dumontet
authors.forename: Christian
authors.initials: C
authors.lastname: Perigaud