title: Identification of the asparagine synthase responsible for D-Asp amidation in the Lactococcus lactis peptidoglycan interpeptide crossbridge.
abstract: We show that in Lactococcus lactis, the gene asnH encodes the asparagine synthase involved in amidation of D-Asp present in peptidoglycan side chains and crossbridges. The level of D-Asp amidation in peptidoglycan has a strong effect on the sensitivity of bacteria to endogenous autolysins and to the cationic antimicrobials nisin and lysozyme.
authors.affiliation: INRA, UR888 Bactéries Lactiques et Pathogènes Opportunistes, F-78350 Jouy-en-Josas, France.
authors.forename: Patrick
authors.initials: P
authors.lastname: Veiga
authors.forename: Michael
authors.initials: M
authors.lastname: Erkelenz
authors.forename: Elvis
authors.initials: E
authors.lastname: Bernard
authors.forename: Pascal
authors.initials: P
authors.lastname: Courtin
authors.forename: Saulius
authors.initials: S
authors.lastname: Kulakauskas
authors.forename: Marie-Pierre
authors.initials: MP
authors.lastname: Chapot-Chartier