title: A novel co-polymer based on hydroxypropyl alpha-cyclodextrin conjugated to low molecular weight polyethylenimine as an in vitro gene delivery vector.
abstract: A novel co-polymer based on 2-hydroxypropyl-alpha-cyclodextrin cross-linked by low molecular weight polyethylenimine was synthesized as a gene delivery vector. The copolymer could bind and condense DNA tightly. It showed lower cytotoxicity than PEI 25kDa in SK-BR-3 cells. Transfection efficiency was increased over 5.5-fold higher than PEI 25 kDa in SK-BR-3 cells in complete serum medium. It is a potential candidate vector for gene therapy.
authors.affiliation: School of Life Science, Guangdong Pharmaceutical University, Guangzhou Higher Education Mega Center, Guangzhou, P. R. China.
authors.forename: Hongliang
authors.initials: H
authors.lastname: Huang
authors.affiliation: Institute of Immunology, Zhejiang University, Yuhangtang Road 388, Hangzhou, P. R. China.
authors.forename: Hai
authors.initials: H
authors.lastname: Yu
authors.affiliation: Institute of Immunology, Zhejiang University, Yuhangtang Road 388, Hangzhou, P. R. China.
authors.forename: Da
authors.initials: D
authors.lastname: Li
authors.affiliation: Institute of Immunology, Zhejiang University, Yuhangtang Road 388, Hangzhou, P. R. China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Institute of Immunology, Zhejiang University, Yuhangtang Road 388, Hangzhou, P. R. China.
authors.forename: Fenping
authors.initials: F
authors.lastname: Shen
authors.affiliation: Institute of Chemical Biology and Pharmaceutical Chemistry, Zhejiang University, Tianmushan Road 148, Hangzhou, P. R. China.
authors.forename: Jun
authors.initials: J
authors.lastname: Zhou
authors.affiliation: Institute of Immunology, Zhejiang University, Yuhangtang Road 388, Hangzhou, P. R. China.
authors.forename: Qingqing
authors.initials: Q
authors.lastname: Wang
authors.affiliation: Institute of Chemical Biology and Pharmaceutical Chemistry, Zhejiang University, Tianmushan Road 148, Hangzhou, P. R. China.
authors.forename: Guping
authors.initials: G
authors.lastname: Tang