A conformational transition in the structure of a 2'-thiomethyl-modified DNA visualized at high resolution.
Crystal structures of A-form and B-form DNA duplexes containing 2'-S-methyl-uridines reveal that the modified residues adopt a RNA-like C3'-endo pucker, illustrating that the replacement of electronegative oxygen at the 2'-carbon of RNA by sulfur does not appear to fundamentally alter the conformational preference of the sugar in the oligonucleotide context and sterics trump stereoelectronics.
Pallan
Pradeep S
PS
Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, TN 37232, USA.
Prakash
Thazha P
TP
Li
Feng
F
Eoff
Robert L
RL
Manoharan
Muthiah
M
Egli
Martin
M