title: Splicing of the large intron present in the nonstructural gene of minute virus of mice is governed by TIA-1/TIAR binding downstream of the nonconsensus donor.
abstract: The essential proteins NS1 and NS2 of minute virus of mice are encoded by mRNAs R1 and R2, respectively. R2 is derived from R1 by excision of a large intron and thus splicing governs the relative ratios of NS1 and NS2. Excision of the large intron utilizes a nonconsensus 5' donor site. We identified a U-rich and A-rich intronic sequence immediately downstream of the nonconsensus 5' donor site that functions as an intronic splicing enhancer (ISE) required for efficient large-intron excision. The ISE binds the cellular RNA-processing proteins TIA-1 and TIAR, which enhance usage of the nonconsensus donor.
authors.affiliation: Department of Molecular Microbiology and Immunology, University of Missouri-Columbia School of Medicine, Life Sciences Center, Columbia, Missouri 65211, USA.
authors.forename: Eun-Young
authors.initials: EY
authors.lastname: Choi
authors.forename: David
authors.initials: D
authors.lastname: Pintel