title: Development of loop-mediated isothermal amplification assay for detection of Entamoeba histolytica.
abstract: A novel one-step, closed-tube, loop-mediated isothermal amplification (LAMP) assay for detecting Entamoeba histolytica, one of the leading causes of morbidity in developing countries, was developed. The sensitivity of the LAMP assay is 1 parasite per reaction. A total of 130 clinical samples were analyzed, and the results compared with those of conventional nested PCR to validate the practicability of this assay. No DNA was amplified from other diarrheal pathogens, such as other Entamoeba species, bacteria, and viruses. These results indicate that LAMP is a rapid, simple, and valuable diagnostic tool for epidemiological studies of amebiasis.
authors.affiliation: Institute of Oral Biology, School of Dentistry, National Yang-Ming University, Taipei, Taiwan.
authors.forename: Shih-Yu
authors.initials: SY
authors.lastname: Liang
authors.forename: Yun-Hsien
authors.initials: YH
authors.lastname: Chan
authors.forename: Kan-Tai
authors.initials: KT
authors.lastname: Hsia
authors.forename: Jing-Lun
authors.initials: JL
authors.lastname: Lee
authors.forename: Ming-Chu
authors.initials: MC
authors.lastname: Kuo
authors.forename: Kuo-Yuan
authors.initials: KY
authors.lastname: Hwa
authors.forename: Chi-Wen
authors.initials: CW
authors.lastname: Chan
authors.forename: Ting-Yi
authors.initials: TY
authors.lastname: Chiang
authors.forename: Jung-Sheng
authors.initials: JS
authors.lastname: Chen
authors.forename: Fang-Tzy
authors.initials: FT
authors.lastname: Wu
authors.forename: Dar-Der
authors.initials: DD
authors.lastname: Ji