[Role of exogenic precursors in formation of surface-active substances during cultivation Rhodoococcus erythiropolis EK-1 on ethanol].
A possibility to intensify synthesis of surfactants of Rhodococcus erythropolis EK-1 under the presence of citrate (lipid synthesis regulator) and fumarate (gluconeogenesis precursor) has been shown. A 40-100% increase of indices of surfactants synthesis with introduction of cytrate (0.1%) and fumerate (0.2%) in the beginning of the stationary producer growth phase is determined by activation of gluconeogenetic branch of metabolism and by intensification of lipids synthesis that was evidenced by the 1.4-1.5-fold and 3.4-3.6-fold increase of isocitrate liase and phosphoenolpyruvate synthetase activities, respectively, as well as by a 1.5-1.6-fold decrease of activity of isocitrate dehydrogenase. The paper is presented in Russian.
T P
TP
Pyroh
Iu V
IuV
Korzh
T A
TA
Shevchuk
D O
DO
Tarasenko
D019343:Citric Acid / Q000737:chemistry / Q000494:pharmacology*; D003470:Culture Media / Q000737:chemistry*; D000431:Ethanol / Q000737:chemistry*; D005650:Fumarates / Q000737:chemistry / Q000494:pharmacology*; D005943:Gluconeogenesis / Q000187:drug effects; D007522:Isocitrate Lyase / Q000378:metabolism; D050356:Lipid Metabolism / Q000187:drug effects; D008055:Lipids / Q000096:biosynthesis; D017866:Phosphotransferases (Paired Acceptors) / Q000378:metabolism; D012240:Rhodococcus / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D013501:Surface-Active Agents / Q000378:metabolism*
D003470:Culture Media; D005650:Fumarates; D008055:Lipids; D013501:Surface-Active Agents; D019343:Citric Acid; D000431:Ethanol; D017866:Phosphotransferases (Paired Acceptors); C022509:pyruvate, water dikinase; D007522:Isocitrate Lyase