[Effect of Myostatin gene vaccine on immunized mice].
OBJECTIVE
To characterize the immunological function of Myostatin gene vaccine and observe the effect of Myostatin gene vaccine on the immunized animal.


METHODS
The mice were immunized with Myostatin gene vaccine. We characterized the antibody titer in the immunized mice serum by the ELISA. The anti-serum was detected by auto-biochemistry analysis software. Meanwhile, the effect anti-serum was detected by auto-biochemistry analysis software. Meanwhile, the effect of Myostatin gene vaccine on skeletal-muscle of the immunized mice was analyzed by HE stain. The cross section area of muscle fiber in immunized mice was analyzed by the Scion Image 4.02 software.


RESULTS
Myostatin gene vaccine could induce the anti-serum against Myostatin in immunized mice. Compared with that in the control group, the mean weight in the pVAC-TT-Ms immunized mice group increased with 9.8%. The quadriceps muscle, gastrocnemius muscle and pectoralis magor in immunized mice increased with 24.1%, 10.9% and 20.3%, respectively.


CONCLUSIONS
Myostatin gene vaccine induced the specific antibody against Myostatin, and made the muscle strong.
Biotechnology Center of the Fourth Military Medical University, Xi'an 710032, China. wyjz2008@live.cn
Shou-zhen
SZ
Wu
Su-lan
SL
Chen
Li-qing
LQ
He
Kuo
K
Zhang
Liang
L
Tang
Xin
X
Qin
Ying-qi
YQ
Zhang
D000818:Animals; D000906:Antibodies / Q000097:blood; D001835:Body Weight / Q000187:drug effects / Q000276:immunology; D007106:Immune Sera / Q000097:blood / Q000276:immunology; D007114:Immunization; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D018482:Muscle, Skeletal / Q000187:drug effects / Q000276:immunology; D055435:Myostatin / Q000235:genetics* / Q000276:immunology*; D010957:Plasmids / Q000008:administration & dosage / Q000235:genetics; D052097:Quadriceps Muscle / Q000187:drug effects / Q000276:immunology; D019444:Vaccines, DNA / Q000008:administration & dosage / Q000276:immunology*
D000906:Antibodies; D007106:Immune Sera; D055435:Myostatin; D019444:Vaccines, DNA