Fully automated, sequential tilt-series acquisition with Leginon.
Electron tomography has become a uniquely powerful tool for investigating the structures of individual cells, viruses, and macromolecules. Data collection is, however, time consuming and requires expensive instruments. To optimize productivity, we have incorporated one of the existing tilt-series acquisition programs, UCSF Tomo, into the well-developed automatic electron microscopy data collection package Leginon to enable fully automatic, sequential tilt-series acquisition. Here we describe how UCSF Tomo was integrated into Leginon, what users must do to set up a data collection session, how the automatic collection proceeds, how archived data about the process can be accessed and used, and how the software has been tested.
Division of Biology, California Institute of Technology, 1200 E. California Blvd., Pasadena, CA 91125, USA.
Christian
C
Suloway
Jian
J
Shi
Anchi
A
Cheng
James
J
Pulokas
Bridget
B
Carragher
Clinton S
CS
Potter
Shawn Q
SQ
Zheng
David A
DA
Agard
Grant J
GJ
Jensen
D020285:Cryoelectron Microscopy; D055032:Electron Microscope Tomography / Q000379:methods*; D046529:Microscopy, Electron, Transmission; D012984:Software