Control of collagen molecular assembly with anionic polysaccharides.
The effect of adding a polyanionic saccharide (alginic acid, pectic acid, agarose, kappa-carragenan or lambda-carragenan) to a collagen solution on the reconstruction rate of collagen molecular assembly was evaluated. The rate of collagen molecular assembly was reduced by the addition of alginic acid and of pectic acid that was containing carboxy groups. Dextran, agarose, and soluble starch did not affect the rate, while dextran sulfate and lambda-carragenan accelerated the rate of collagen molecular assembly. The denaturation curves of collagen reconstructed with lambda-carragenan showed two peaks at about 45 degrees C and 55 degrees C. The use of a polyanionic saccharide made it possible to endow the reconstructed collagen fibrils with flexibility to enhance the application of collagen as a scaffold material for tissue engineering.
Scleroprotein and Leather Research Institute, Faculty of Agriculture, Tokyo University of Agriculture and Technology, Saiwai-cho, Fuchu, Tokyo 183-8509, Japan. ny318@cc.tuat.ac.jp
Yoshihiro
Y
Nomura
Yasuhiro
Y
Ishii
Koji
K
Takahashi
D003094:Collagen / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D011134:Polysaccharides / Q000737:chemistry* / Q000494:pharmacology*; D011485:Protein Binding / Q000187:drug effects; D011489:Protein Denaturation / Q000187:drug effects; D055550:Protein Stability / Q000187:drug effects; D013696:Temperature
D011134:Polysaccharides; D003094:Collagen