Application of in-line liquid chromatography-accurate radioisotope counting-mass spectrometry (LC-ARC-MS) to evaluate metabolic profile of [3H]-mefenamic acid in rat plasma.
Profiling of rat plasma using a highly sensitive LC-ARC-MS technique showed that [(3)H] mefenamic acid was metabolized to several products, including a sulfate conjugate and a hydroxylated analogue as major metabolites. This technique of detecting low levels of radioactivity in plasma was superior to previously used methods, such as beta-RAM detectors.
Department of Pharmacokinetics, Dynamics, and Metabolism, Pfizer Global Research and Development, 2800 Plymouth Road, Ann Arbor, MI 48105, USA. wing.lam@pfizer.com
Wing
W
Lam
Cho-Ming
CM
Loi
James
J
Atherton
Wayne
W
Stolle
John
J
Easter
Abdul
A
Mutlib
D000818:Animals; D002853:Chromatography, Liquid / Q000379:methods*; D006900:Hydroxylation; D008297:Male; D013058:Mass Spectrometry / Q000379:methods*; D008528:Mefenamic Acid / Q000032:analysis* / Q000493:pharmacokinetics; D011868:Radioisotopes; D051381:Rats; D017207:Rats, Sprague-Dawley; D012588:Scintillation Counting / Q000379:methods; D013431:Sulfates / Q000378:metabolism
D011868:Radioisotopes; D013431:Sulfates; D008528:Mefenamic Acid