Intermittent microwave irradiation facilitates antigen-antibody reaction in Western blot analysis.
We established a shortened protocol for western blot analysis using intermittent microwave irradiation. With this method, the procedure is completed within 1 h after applying the primary antibody, and thus greatly saves time. This procedure appears to be applicable to any antibody based on our experience of several years.
Liu
Yu-Ting
YT
Department of Pathology and Biology of Diseases, Graduate School of Medicine, Kyoto University, Yoshida-Konoe-cho, Sakyo-ku, Kyoto, 606-8501, Japan.
Toyokuni
Shinya
S
D000818:Animals; D000937:Antigen-Antibody Reactions / Q000528:radiation effects*; D015153:Blotting, Western / Q000379:methods*; D008297:Male; D008872:Microwaves; D051381:Rats; D017208:Rats, Wistar