Central metabolism in Mycobacterium smegmatis during the transition from O2-rich to O2-poor conditions as studied by isotopomer-assisted metabolite analysis.
Isotopomer-assisted metabolite analysis was used to investigate the central metabolism of Mycobacterium smegmatis and its transition from normal growth to a non-replicating state under a hypoxic environment. Tween 80 significantly promoted aerobic growth by improving O(2) transfer, while only small amount was degraded and metabolized via the TCA cycle for biomass synthesis. As the bacillus encountered hypoxic stress, isotopomer analysis suggested: (1) isocitrate lyase activity increased, which further induced glyoxylate pathway and glycine dehydrogenase for replenishing NAD(+); (2) the relative amount of acetyl-CoA entering the TCA cycle was doubled, whereas little entered the glycolytic and pentose phosphate pathways.
Department of Energy, Environmental and Chemical Engineering, Washington University, St. Louis, MO 63130, USA. yinjie.tang@wustl.edu
Yinjie J
YJ
Tang
Wenqing
W
Shui
Samuel
S
Myers
Xueyang
X
Feng
Carolyn
C
Bertozzi
Jay D
JD
Keasling
D000105:Acetyl Coenzyme A / Q000378:metabolism; D000222:Adaptation, Physiological; D000332:Aerobiosis; D000693:Anaerobiosis; D002952:Citric Acid Cycle; D050789:Glycine Dehydrogenase / Q000378:metabolism; D007522:Isocitrate Lyase / Q000378:metabolism; D020102:Mycobacterium smegmatis / Q000378:metabolism / Q000502:physiology*; D009243:NAD / Q000378:metabolism; D010427:Pentose Phosphate Pathway; D013312:Stress, Physiological
D009243:NAD; D000105:Acetyl Coenzyme A; D050789:Glycine Dehydrogenase; D007522:Isocitrate Lyase