Involvement of DNA fragmentation of enterocytes in mucosal injury to a mouse jejunum incubated in ussing chambers.
A mouse jejunum, when incubated in vitro in Ussing chambers, was found to exhibit morphological deterioration of the villi with denudation of the epithelia (J Nutr Sci Vitaminol, 51: 406, 2005). Our study examined the involvement of apoptosis in an intestinal injury model by a DNA ladder assay and terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling (TUNEL) assay. Electrophoresis of mucosal DNA revealed ladders, indicating the occurrence of DNA fragmentation. Cells with TUNEL-positive nuclei were detected among the villus epithelial cells (enterocytes), whereas they are rarely seen among crypt epithelial cells. These features were evident within 1 h after the start of incubation. Apoptotic death of the enterocytes was thus involved in the destruction of villi when incubated in Ussing chambers.
Department of Nutritional Sciences, School of Nutritional Sciences, Nagoya University of Arts and Sciences, 57 Takenoyama, Iwasaki-cho, Nisshin, Aichi 470-0196, Japan. e-ina@nuas.ac.jp
Eiko
E
Inagaki-Tachibana
Takamitsu
T
Tsukahara
Kazuhiko
K
Kaji
Ryoji
R
Eguchi
Hiroaki
H
Kanazawa
Hisayoshi
H
Hayashi
Yuichi
Y
Suzuki
D000818:Animals; D021461:Animals, Outbred Strains; D017209:Apoptosis; D053938:DNA Fragmentation; D015194:Diffusion Chambers, Culture; D020895:Enterocytes / Q000473:pathology*; D020287:In Situ Nick-End Labeling; D066298:In Vitro Techniques; D007413:Intestinal Mucosa / Q000473:pathology*; D007583:Jejunum / Q000473:pathology*; D008297:Male; D051379:Mice