PhiC31 integrase and liver-specific regulatory elements confer high-level, long-term expression of firefly luciferase in mouse liver.
To improve the efficiency of expression of reporter transgenes delivered by hydrodynamic injection, we generated expression cassettes carrying different liver-specific regulatory elements using the firefly luciferase gene as a reporter. From our studies the human alpha-antitrypsin promoter together with the apolipoprotein E/C-I and albumin enhancer was the combination of choice for prolonged transgene expression, but reporter gene expression in vivo lasted for no more than 7 weeks. Subsequently, phage phiC31 integrase was introduced as a potential tool to improve further the efficiency of expression of the delivered transgene. Long-term transgene expression in vivo was achieved by specific integration of the target gene into mouse livers. This study demonstrates the use of a combination of phage phiC31 integrase and liver-specific regulatory elements for generation of transgenic mice.
Fu
Qiuxia
Q
Laboratory of Blood-Borne Virus, Beijing Institute of Transfusion Medicine, 27(9) Tai Ping Road, 100850, Beijing, China.
Jia
Shuaizheng
S
Sun
Zhidong
Z
Tian
Fenmei
F
Du
Juan
J
Zhou
Yong
Y
Wang
Yingli
Y
Wang
Xiaohui
X
Zhan
Linsheng
L
D000818:Animals; D001435:Bacteriophages / Q000201:enzymology* / Q000235:genetics; D005822:Genetic Vectors; D006801:Humans; D019426:Integrases / Q000235:genetics*; D008099:Liver / Q000201:enzymology*; D049409:Luciferases, Firefly / Q000096:biosynthesis* / Q000235:genetics; D051379:Mice; D008822:Mice, Transgenic / Q000235:genetics; D050436:Regulatory Elements, Transcriptional; D014764:Viral Proteins / Q000235:genetics*
D014764:Viral Proteins; D049409:Luciferases, Firefly; D019426:Integrases