A two-step method to covalently bind biomolecules to group-IV semiconductors: Si(111)/1,2-epoxy-9-decene/esterase.
A versatile two-step method has been developed that allows linking of biomolecules covalently to hydrogen-terminated group-IV semiconductors by means of epoxy-alkenes. First, the terminal C==C double bond of the alkene forms a covalent bond with the silicon, germanium, or diamond surface by UV-mediated hydrosilylation. The terminal oxirane moiety then reacts with the biomolecule. As a model system, we investigated the attachment of an esterase B to a Si(111) surface by means of the linker molecule 1,2-epoxy-9-decene. Samples were characterized by X-ray photoelectron spectroscopy (XPS) and Fourier transform infrared (FTIR) spectroscopy. The immobilized enzyme retained its activity and exhibited good long-term stability.
Institute for Process Engineering, TU Graz, Inffeldgasse 21A/II, A-8010 Graz, Austria.
Claire
C
Jeanquartier
Gerburg
G
Schider
Sabine
S
Feichtenhofer
Helmut
H
Schwab
Robert
R
Schennach
Johanna
J
Stettner
Adolf
A
Winkler
Heidrun
H
Gruber-Woelfler
Georg
G
Schitter
Rafael J P
RJ
Eder
Johannes G
JG
Khinast
D004852:Epoxy Compounds / Q000737:chemistry*; D004950:Esterases / Q000737:chemistry* / Q000378:metabolism*; D008958:Models, Molecular; D012666:Semiconductors; D012825:Silicon / Q000737:chemistry*; D013057:Spectrum Analysis
D004852:Epoxy Compounds; D004950:Esterases; D012825:Silicon