Scale-up of counter-current chromatography: demonstration of predictable isocratic and quasi-continuous operating modes from the test tube to pilot/process scale.
Predictable scale-up from test tube derived distribution ratios and analytical-scale sample loading optimisation is demonstrated using a model sample system of benzyl alcohol and p-cresol in a heptane:ethyl acetate:methanol:water phase system with the new 18 L Maxi counter-current chromatography centrifuge. The versatility of having a liquid stationary phase with its high loading capacity and flexible operating modes is demonstrated at two different scales by separating and concentrating target compounds using a mixture of caffeine, vanillin, naringenin and carvone using a quasi-continuous technique called intermittent counter-current extraction.
Sutherland
Ian
I
Brunel Institute for Bioengineering, Brunel University, Uxbridge, Middlesex UB83PH, UK. ian.sutherland@brunel.ac.uk
Hewitson
Peter
P
Ignatova
Svetlana
S
D019905:Benzyl Alcohol / Q000302:isolation & purification; D002498:Centrifugation; D003377:Countercurrent Distribution / Q000295:instrumentation*; D003408:Cresols / Q000302:isolation & purification; D010865:Pilot Projects
D003408:Cresols; C032538:4-cresol; D019905:Benzyl Alcohol