Using morpholinos for gene knockdown in Giardia intestinalis.
We used translation-blocking morpholinos to reduce protein levels in Giardia intestinalis. Twenty-four hours after electroporation with morpholinos targeting either green fluorescent protein or kinesin-2b, levels of these proteins were reduced by 60%. An epitope-tagged transgene can also be used as a reporter for morpholino efficacy with targets lacking specific antibodies.
Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720, USA.
Meredith L
ML
Carpenter
W Zacheus
WZ
Cande
D000818:Animals; D055785:Gene Knockdown Techniques; D020868:Gene Silencing; D016829:Giardia lamblia / Q000235:genetics* / Q000378:metabolism; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D016376:Oligonucleotides, Antisense / Q000235:genetics*
D016376:Oligonucleotides, Antisense; D049452:Green Fluorescent Proteins