Chemiluminescence determination of tetracyclines using Fenton system in the presence europium(III) ions.
A new simple chemiluminescent method for the determination of chlortetracycline (Chlor-TC), oxytetracycline (Oxy-TC) and doxycycline (Doxy-TC) is described. This method is based on the europium(III) emission as a result of the energy transfer process from the excited product of the tetracyclines oxidation to the uncomplexed Eu(III). Under the optimum conditions, calibration graphs were obtained for 4x10(-7) to 2x10(-5) mol L(-1) of Chlor-TC; 2x10(-7) to 2x10(-5) mol L(-1) of Oxy-TC and 1x10(-7) to 3x10(-5) mol L(-1) of Doxy-TC. The method was successfully applied to the determination of these drugs in pharmaceutical and veterinary formulation and honey.
Department of Rare Earths, Faculty of Chemistry, Adam Mickiewicz University, Grunwaldzka 6, 60 - 780 Poznań, Poland.
Malgorzata
M
Kaczmarek
Stefan
S
Lis
D005063:Europium / Q000737:chemistry*; D006722:Honey / Q000032:analysis; D006861:Hydrogen Peroxide / Q000737:chemistry*; D007501:Iron / Q000737:chemistry*; D007700:Kinetics; D049449:Luminescence; D008163:Luminescent Measurements / Q000379:methods*; D004364:Pharmaceutical Preparations / Q000737:chemistry; D013754:Tetracyclines / Q000032:analysis* / Q000737:chemistry; D014867:Water / Q000737:chemistry
C045076:Fenton's reagent; D004364:Pharmaceutical Preparations; D013754:Tetracyclines; D014867:Water; D005063:Europium; D006861:Hydrogen Peroxide; D007501:Iron