Parallel RNA-strand recognition by 2'-amino-beta-L-LNA.
A short synthetic route to the first beta-l-ribo configured locked nucleic acid (LNA), that is, 2'-amino-beta-l-LNA thymine phosphoramidite 6, has been developed from bicyclic nucleoside 1. Incorporation of 2'-amino-beta-l-LNA thymine monomers into alpha-DNA strands results in probes forming stable duplexes with complementary RNA in parallel orientation.
Nucleic Acid Center, Department of Physics and Chemistry, University of Southern Denmark, DK-5230 Odense M, Denmark.
T Santhosh
TS
Kumar
Michael E
ME
Ostergaard
Pawan K
PK
Sharma
Poul
P
Nielsen
Jesper
J
Wengel
Patrick J
PJ
Hrdlicka
D004247:DNA / Q000737:chemistry; D006358:Hot Temperature; D008956:Models, Chemical; D015394:Molecular Structure; D009690:Nucleic Acid Conformation; D009691:Nucleic Acid Denaturation; D009841:Oligonucleotides / Q000737:chemistry*; D012313:RNA / Q000737:chemistry*; D013696:Temperature; D013941:Thymine / Q000737:chemistry
D009841:Oligonucleotides; C477371:locked nucleic acid; D012313:RNA; D004247:DNA; D013941:Thymine