Protein-coding gene promoters in Methanocaldococcus (Methanococcus) jannaschii.
Although Methanocaldococcus (Methanococcus) jannaschii was the first archaeon to have its genome sequenced, little is known about the promoters of its protein-coding genes. To expand our knowledge, we have experimentally identified 131 promoters for 107 protein-coding genes in this genome by mapping their transcription start sites. Compared to previously identified promoters, more than half of which are from genes for stable RNAs, the protein-coding gene promoters are qualitatively similar in overall sequence pattern, but statistically different at several positions due to greater variation among their sequences. Relative binding affinity for general transcription factors was measured for 12 of these promoters by competition electrophoretic mobility shift assays. These promoters bind the factors less tightly than do most tRNA gene promoters. When a position weight matrix (PWM) was constructed from the protein gene promoters, factor binding affinities correlated with corresponding promoter PWM scores. We show that the PWM based on our data more accurately predicts promoters in the genome and transcription start sites than could be done with the previously available data. We also introduce a PWM logo, which visually displays the implications of observing a given base at a position in a sequence.
Department of Microbiology, University of Illinois at Urbana-Champaign, 601 South Goodwin Avenue, Urbana, IL 61801, USA.
Jian
J
Zhang
Enhu
E
Li
Gary J
GJ
Olsen
D019843:Archaeal Proteins / Q000235:genetics*; D001665:Binding Sites; D015870:Gene Expression; D017015:Methanococcales / Q000235:genetics*; D011401:Promoter Regions, Genetic; D012343:RNA, Transfer / Q000235:genetics; D017422:Sequence Analysis, DNA; D035165:Transcription Factors, General / Q000378:metabolism; D024363:Transcription Initiation Site
D019843:Archaeal Proteins; D035165:Transcription Factors, General; D012343:RNA, Transfer