Identification and characterization of a novel gene involved in the trans-specific nematicidal activity of Photorhabdus luminescens LN2.
Photorhabdus luminescens subsp. akhurstii LN2 from Heterorhabditis indica LN2 showed nematicidal activity against axenic Heterorhabditis bacteriophora H06 infective juveniles (IJs). Transposon mutagenesis identified an LN2 mutant that supports the growth of H06 nematodes. Tn5 disrupted the namA gene, encoding a novel 364-residue protein and involving the nematicidal activity. The green fluorescent protein-labeled namA mutant was unable to colonize the intestines of H06 IJs.
Sun Yat-sen University, Guangzhou, China.
Xuehong
X
Qiu
Richou
R
Han
Xun
X
Yan
Mingxing
M
Liu
Li
L
Cao
Toyoshi
T
Yoshiga
Eizo
E
Kondo
D000595:Amino Acid Sequence; D000818:Animals; D000969:Antinematodal Agents / Q000633:toxicity*; D001426:Bacterial Proteins / Q000235:genetics* / Q000633:toxicity; D001483:Base Sequence; D004251:DNA Transposable Elements; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D055786:Gene Knockout Techniques; D023061:Gene Order; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D020637:Photorhabdus / Q000235:genetics* / Q000472:pathogenicity; D017172:Rhabditoidea / Q000187:drug effects*; D017422:Sequence Analysis, DNA; D037521:Virulence Factors / Q000235:genetics* / Q000633:toxicity
D000969:Antinematodal Agents; D001426:Bacterial Proteins; D004251:DNA Transposable Elements; D004269:DNA, Bacterial; D037521:Virulence Factors