ADAMTS-9 expression is up-regulated following transient middle cerebral artery occlusion (tMCAo) in the rat.
The ADAMTS enzymes (a disintegrin and metalloproteinase with thrombospondin type 1-like motifs) have important roles in central nervous system (CNS) physiology and pathology. This current study aimed to analyse the expression of ADAMTS-9 following transient middle cerebral artery occlusion (tMCAo) in the rat, a model of focal cerebral ischaemia. Using real-time RT-PCR, ADAMTS-9 mRNA was demonstrated to be significantly up-regulated in tMCAo brain tissue compared to sham-operated at 24h post-ischaemia. The mature form of the ADAMTS-9 protein was only detected by Western blotting in brains subjected to tMCAo at 24h. In situ hybridisation demonstrated that ADAMTS-9 mRNA was expressed by neurones in tMCAo tissue. This study indicates that ADAMTS-9 expression is modulated in response to cerebral ischaemia in vivo and further research will resolve whether it plays a role in the subsequent degenerative or repair processes.
Reid
Martin J
MJ
Biomedical Research Centre, Sheffield Hallam University, Sheffield, UK. MR469@medschl.cam.ac.uk
Cross
Alison K
AK
Haddock
Gail
G
Allan
Stuart M
SM
Stock
Chris J
CJ
Woodroofe
M Nicola
MN
Buttle
David J
DJ
Bunning
Rowena A D
RA
D051722:ADAM Proteins / Q000378:metabolism*; D000818:Animals; D015153:Blotting, Western; D001921:Brain / Q000201:enzymology*; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D020244:Infarction, Middle Cerebral Artery / Q000201:enzymology*; D002546:Ischemic Attack, Transient / Q000201:enzymology*; D008297:Male; D009474:Neurons / Q000201:enzymology; D017966:Pyramidal Cells / Q000201:enzymology; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015854:Up-Regulation; D014841:von Willebrand Factor / Q000276:immunology / Q000378:metabolism
D005904:Glial Fibrillary Acidic Protein; D012333:RNA, Messenger; C029417:Von Willebrand antigen; D014841:von Willebrand Factor; D051722:ADAM Proteins