Molecular and functional characterization of adipokinetic hormone receptor and its peptide ligands in Bombyx mori.
Neuropeptides of the adipokinetic hormone (AKH) family are among the best studied hormone peptides, but its signaling pathways remain to be elucidated. In this study, we molecularly characterized the signaling of Bombyx AKH receptor (AKHR) and its peptide ligands in HEK293 cells. In HEK293 cells stably expressing AKHR, AKH1 stimulation not only led to a ligand concentration dependent mobilization of intracellular Ca(2+) and cAMP accumulation, but also elicited transient activation of extracellular signal-regulated kinase 1/2 (ERK1/2) pathway. We observed that AKH receptor was rapidly internalized after AKH1 stimulation. We further demonstrated that AKH2 exhibited high activities in cAMP accumulation and ERK1/2 activation on AKHR comparable to AKH1, whereas AKH3 was much less effective.
Institute of Biochemistry, College of Life Sciences, Zijingang Campus, Zhejiang University, Hangzhou, Zhejiang 310058, China.
Chenggang
C
Zhu
Haishan
H
Huang
Rongsheng
R
Hua
Guo
G
Li
Dong
D
Yang
Jiansong
J
Luo
Cunxin
C
Zhang
Liangen
L
Shi
Jeffrey L
JL
Benovic
Naiming
N
Zhou
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D012831:Bombyx / Q000378:metabolism*; D002118:Calcium / Q000378:metabolism; D002460:Cell Line; D000242:Cyclic AMP / Q000378:metabolism; D017931:DNA Primers; D005434:Flow Cytometry; D006801:Humans; D007301:Insect Hormones / Q000737:chemistry / Q000378:metabolism*; D008024:Ligands; D020935:MAP Kinase Signaling System; D008856:Microscopy, Fluorescence; D008969:Molecular Sequence Data; D009842:Oligopeptides / Q000737:chemistry / Q000378:metabolism*; D010446:Peptide Fragments / Q000378:metabolism*; D011761:Pyrrolidonecarboxylic Acid / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism; D018027:Receptors, Glucagon / Q000737:chemistry / Q000378:metabolism*
D017931:DNA Primers; D007301:Insect Hormones; D008024:Ligands; D009842:Oligopeptides; D010446:Peptide Fragments; D018027:Receptors, Glucagon; C044833:adipokinetic hormone; D000242:Cyclic AMP; D002118:Calcium; D011761:Pyrrolidonecarboxylic Acid