Effect of amino acid availability on vitamin B12 production in Lactobacillus reuteri.
Recent functional genomics and genome-scale modeling approaches indicated that B(12) production in Lactobacillus reuteri could be improved by optimization of the medium. Here we show that a series of systematic single-amino-acid omissions could significantly modulate the production of B(12) from nearly undetectable levels (with omission of isoleucine) to levels 20-fold higher than the levels previously reported (with omission of cysteine). Using cDNA microarray experiments, we analyzed the transcriptional response of L. reuteri to medium lacking cysteine. The results supported the observed high level of B(12) production and provided new avenues for future improvement of production of vitamin B(12).
Santos
Filipe
F
Kluyver Centre for Genomics of Industrial Fermentation, TI Food and Nutrition, and NIZO food research, BA Ede, The Netherlands.
Teusink
Bas
B
Molenaar
Douwe
D
van Heck
Maurice
M
Wels
Michiel
M
Sieuwerts
Sander
S
de Vos
Willem M
WM
Hugenholtz
Jeroen
J
D000596:Amino Acids / Q000378:metabolism*; D003470:Culture Media / Q000737:chemistry; D020869:Gene Expression Profiling; D052200:Limosilactobacillus reuteri / Q000378:metabolism*; D020411:Oligonucleotide Array Sequence Analysis; D014805:Vitamin B 12 / Q000096:biosynthesis*
D000596:Amino Acids; D003470:Culture Media; D014805:Vitamin B 12