Autoinduction of protein expression.
This unit contains protocols for the use of lactose-derived autoinduction in Escherichia coli. The protocols allow for reproducible expression trials to be undertaken with minimal user intervention. A basic protocol covers production of unlabeled proteins for functional studies. Alternate protocols for selenomethionine labeling for X-ray structural studies, and multi-well plate growth for screening and optimization are also included.
Fox
Brian G
BG
Department of Biochemistry, Biophysics Degree Program, and Center for Eukaryotic Structural Genomics, University of Wisconsin-Madison, Madison, Wisconsin.
Blommel
Paul G
PG
Department of Biochemistry, Biophysics Degree Program, and Center for Eukaryotic Structural Genomics, University of Wisconsin-Madison, Madison, Wisconsin.
D001426:Bacterial Proteins / Q000096:biosynthesis*; D003001:Cloning, Molecular; D018360:Crystallography, X-Ray; D003470:Culture Media; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D007785:Lactose / Q000378:metabolism; D011994:Recombinant Proteins / Q000096:biosynthesis*; D012645:Selenomethionine / Q000378:metabolism
D001426:Bacterial Proteins; D003470:Culture Media; D011994:Recombinant Proteins; D012645:Selenomethionine; D007785:Lactose