Overexpression of Cks1 is associated with poor survival by inhibiting apoptosis in breast cancer.
OBJECTIVE
The present study was undertaken to examine the amplification and expression status of Cks1 in breast cancer and its significance.


METHODS
The amplification and expression status of Cks1 gene was examined by FISH, real-time PCR and immunohistochemistry, respectively. RNA interference was used to detect the effects of Cks1 on migration, invasion, cell cycle progress and apoptosis of breast cancer cells.


RESULTS
Cks1 gene amplification was highly correlated with protein overexpression. Overexpression of Cks1 was strongly associated with lymph node metastasis and poor prognosis (P = 0.000, 95% CI (0.00-0.02); P = 0.008, 95% CI (0.001-0.05), respectively). Knockdown of Cks1 expression by RNA interference inhibited the cell cycle progress, migration and invasion ability of breast cancer cells. Moreover, overexpression of Cks1 inhibited apoptosis of breast cancer cells through MEK-Erk pathway.


CONCLUSIONS
Cks1 may be considered as potential novel prognostic markers and targets for the future development of specific therapeutic interventions in breast cancer.
Wang
Xiao-Chun
XC
Institute of Radiation Medicine, Chinese Academy of Medical Science, 300192 Tianjin, China. wxc3188@126.com
Tian
Li-Li
LL
Tian
Jing
J
Wu
Hai-Liang
HL
Meng
Ai-Min
AM
D017209:Apoptosis; D015153:Blotting, Western; D001943:Breast Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000401:mortality*; D042846:CDC2-CDC28 Kinases; D002352:Carrier Proteins / Q000235:genetics* / Q000378:metabolism*; D002465:Cell Movement; D049109:Cell Proliferation; D018844:Cyclin-Dependent Kinases / Q000235:genetics* / Q000378:metabolism*; D005260:Female; D005434:Flow Cytometry; D005784:Gene Amplification; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007124:Immunoenzyme Techniques; D017404:In Situ Hybridization, Fluorescence; D008207:Lymphatic Metastasis; D048369:MAP Kinase Kinase 1 / Q000378:metabolism; D008875:Middle Aged; D019950:Mitogen-Activated Protein Kinase 1 / Q000378:metabolism; D048052:Mitogen-Activated Protein Kinase 3 / Q000378:metabolism; D009361:Neoplasm Invasiveness; D009367:Neoplasm Staging; D011379:Prognosis; D034741:RNA, Small Interfering / Q000494:pharmacology; D015996:Survival Rate
C093090:CKS1B protein, human; D002352:Carrier Proteins; D034741:RNA, Small Interfering; D042846:CDC2-CDC28 Kinases; D018844:Cyclin-Dependent Kinases; D019950:Mitogen-Activated Protein Kinase 1; D048052:Mitogen-Activated Protein Kinase 3; D048369:MAP Kinase Kinase 1; C482125:MAP2K1 protein, human