[Chemoattractive effects of chemokine-like factor 1 on human arterial smooth muscle cells].
OBJECTIVE
To investigate the chemotactic effect of chemokine-like factor 1 (CKLF1) on human arterial smooth muscle cells (ASMCs).


METHODS
The recombinant eukaryotic expression vectors pEGFP-N1-CKLF1 (test group) and pEGFP-N1 (control group) were transiently transfected into 293T cells. The supernatants were harvested 72 h after transfection for bioactivity study. The chemotactic effect of CKLF1 on ASMCs were assayed by cellular chemotactic experiments.


RESULTS
ASMCs number migrated from the test and control groups diluted by none and 10-fold supernatants had statistical significance (114+/-4 vs 41+/-4, P<0.05; 74+/-4 vs 34+/-3, P<0.01), but have no statistical significance (28+/-4 vs 25+/-5, P>0.05; 26+/-5 vs 23+/-5, P>0.05) between the two groups diluted by 100-fold and 1 000-fold supernatants. When ASMCs were treated at different concentrations of 0 and 2 microg/L of pertussis toxin (PTX), the cell number migrated from the test and control groups diluted by 10-fold supernatants, they had statistical significance (74+/-4 vs 34+/-3, P<0.01; 45+/-3 vs 34+/-3, P<0.01). And when ASMCs were treated at 10 microg/L of PTX, the cell number migrated from both groups diluted by 10-fold supernatants, they had no statistical significance (37+/-4 vs 34+/-3, P>0.05).


CONCLUSIONS
CKLF1 has significant chemotactic effects on ASMCs and such a CKLF1-induced chemotaxis could be inhibited by PTX at concentration of 10 microg/L.
Xuan
Cheng-rui
CR
Department of Vascular Surgery, Peking University People's Hospital, Beijing 100044, China.
Chen
Yao
Y
He
Pei-ying
PY
Shen
Chen-yang
CY
D002478:Cells, Cultured; D018925:Chemokines / Q000096:biosynthesis* / Q000235:genetics; D002633:Chemotaxis / Q000187:drug effects*; D005822:Genetic Vectors; D006801:Humans; D007083:Iliac Artery / Q000166:cytology; D062792:MARVEL Domain-Containing Proteins; D009131:Muscle, Smooth, Vascular / Q000166:cytology* / Q000378:metabolism*; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics; D014162:Transfection
C432676:CKLF protein, human; D018925:Chemokines; D062792:MARVEL Domain-Containing Proteins; D011994:Recombinant Proteins