Immobilization of lactobionic acid on the surface of cadmium sulfide nanoparticles and their interaction with hepatocytes.
In the current study, beta-galactose-carrying lactobionic acid (LA) was conjugated on the surface of mercaptoacetic acid-coated cadmium sulfide nanoparticles (CSNPs) to ensure specific recognition of liver cells (hepatocytes) and to enhance biocompatibility. Maltotrionic acid-coated CSNPs (MCSNPs) were also prepared for use as a control. The results showed that LA-immobilized CSNPs (LCSNPs) were selectively and rapidly internalized into hepatocytes and emitted more intense fluorescence images as well as demonstrated increased biocompatible behavior in vitro than those of CSNPs and MCSNPs. Furthermore, the uptake amount of LCSNPs into hepatocytes was higher than that of CSNPs and MCSNPs. All these results indicate that LCSNPs may find ever-growing applications in biological labels and detection or contrast agents in life science and medical diagnostics.
Department of Polymer Science, Kyungpook National University, Daegu, 702-701, South Korea.
K M
KM
Kamruzzaman Selim
Zhi-Cai
ZC
Xing
Haiqing
H
Guo
Inn-Kyu
IK
Kang
D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry; D019187:Cadmium Compounds / Q000737:chemistry*; D018929:Cell Culture Techniques / Q000295:instrumentation; D002470:Cell Survival; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D004187:Disaccharides / Q000737:chemistry*; D022781:Hepatocytes / Q000166:cytology / Q000378:metabolism; D053758:Nanoparticles / Q000737:chemistry*; D036103:Nanotechnology / Q000379:methods*; D051381:Rats; D017207:Rats, Sprague-Dawley; D017550:Spectroscopy, Fourier Transform Infrared; D013440:Sulfides / Q000737:chemistry*; D013499:Surface Properties; D001616:beta-Galactosidase / Q000737:chemistry
D001672:Biocompatible Materials; D019187:Cadmium Compounds; D020099:Coated Materials, Biocompatible; D004187:Disaccharides; D013440:Sulfides; C034939:cadmium sulfide; C005608:lactobionic acid; D001616:beta-Galactosidase