Blotting from PhastGel to membranes by ultrasound.
Ultrasound-based approach for enhanced protein blotting is proposed. Three minutes of ultrasound exposure (1 MHz, 2.5 W/cm(2)) was sufficient for a clear transfer of proteins from a polyacrylamide gel (PhastGel) to nitrocellulose or Nylon 66 Biotrans membrane. The proteins evaluated were prestained sodium dodecyl sulfate-polyacrylamide standards (18,500-106,000 Da) and (14)C-labeled Rainbow protein molecular weight markers (14,300-200,000 Da).
Department of Chemical Engineering, Ben-Gurion University, Beer-Sheva, 84105, Israel. kost@bgu.ac.il
Joseph
J
Kost
D015153:Blotting, Western / Q000295:instrumentation / Q000379:methods; D004591:Electrophoresis, Polyacrylamide Gel / Q000295:instrumentation / Q000379:methods; D006801:Humans; D011506:Proteins / Q000032:analysis; D014465:Ultrasonics
D011506:Proteins