[Potential application of human amniotic mesenchymal cells for seeding cells in bone tissue engineering].
OBJECTIVE
To investigate the potential of human amniotic mesenchymal cells (hAMC) serving as seeding cells in bone tissue engineering.


METHODS
hAMC were isolated and cultured. The third passage of hAMC was cultured in osteogenic induced media [DMEM supplemented with 10% (v/v) FBS, 0.1 mumol/L dexamethasone, 50 mg/L ascorbic acid and 10 mmol/L beta-glycerophosphate] for one week. Calcified nodules were shown by alizarin red staining and counted under light microscope. Immunofluorescence cytochemical staining was used to detect collagen I (COL I) and alkaline phosphatase (ALP). Expression of FasL was examined in the amnion and hAMC by immunohistochemistry or immunocytochemistry.


RESULTS
After osteoblast differentiation, calcified nodules were formed, on the average 18 per well. hAMC in calcified nodules showed positive expression of COL I and ALP. FasL was detected positive both in cells contained in amnion and in hAMC.


CONCLUSIONS
hAMC are potential ideal candidates for seeding cells in bone tissue engineering.
Department of Anatomy and Histoembryology, Peking University School of Basic Medical Sciences, Beijing 100191, China.
Fang
F
Mei
Jun-min
JM
Tang
Jin-yan
JY
Zhong
Wei-hong
WH
Qi
Yan
Y
Tang
Feng
F
Li
D000469:Alkaline Phosphatase / Q000378:metabolism; D000650:Amnion / Q000166:cytology* / Q000378:metabolism; D002454:Cell Differentiation / Q000502:physiology*; D002478:Cells, Cultured; D024042:Collagen Type I / Q000378:metabolism; D053222:Fas Ligand Protein / Q000378:metabolism; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism; D010006:Osteoblasts / Q000166:cytology*; D010012:Osteogenesis / Q000502:physiology; D023822:Tissue Engineering / Q000379:methods*
D024042:Collagen Type I; C505661:FASLG protein, human; D053222:Fas Ligand Protein; D000469:Alkaline Phosphatase