[Protein restriction in pregnancy: effects related to dam metabolism].
Metabolism alterations were evaluated in female Wistar rats (dams) during pregnancy. Pregnant and non-pregnant dams submitted to protein restriction, were fed isocaloric (15.74 kJ/g), control or hypoproteic (17% vs. 6%) diets, and distributed in four Groups (n=7) as follows: non-pregnant control (NPC), pregnant control (PC), non-pregnant hypoproteic (NPH), and pregnant hypoproteic (PH); from Day 1 to Day 18 of pregnancy. Biochemical, hormonal and metabolic parameters related to lipid synthesis were assessed. The two-way ANOVA, followed by Tukey-HSD and Student-t tests were used, with a significance of p< 0.05. Protein restriction elevated lipid synthesis and malic enzyme (ME) activity in the liver, and reduced mass and the lipid/glycogen ratio in this tissue; it also lowered protein ingestion (total and %), lipid content (%) in the mammary gland (MAG), serum proteins and albumin, with consequent reduction of placenta and fetal masses. Pregnancy reduced serum protein and albumin concentrations, lipid synthesis, ME activity, hepatic lipid and glycogen content. However, it increased final body mass; increased relative masses of gonad (GON), liver and MAG; but reduced lipid synthesis and content of GON, lipid content of MAG and the relative mass of carcass. Pregnancy Insulinemia increased during pregnancy with reduced glycemia, characterizing hormonal resistance. Leptin and prolactin were also increased during pregnancy, being the highest increase in observed in HP rats. Protein restriction in pregnancy modified maternal metabolism, altering lipid synthesis in the liver and hormonal profile and decreasing the placenta and fetus masses.
Ballen
Marcia L O
ML
Curso de Ciências da Saúde, Faculdade de Ciências Médicas, Universidade Federal de Mato Grosso, Av. Fernando Correa da Costa s/n, Coxipó, Cuiabá, MT.
Moretto
Vera L
VL
Santos
Maisa P dos
MP
Gonçalves
Talita S S
TS
Kawashita
Nair H
NH
Stoppiglia
Luis F
LF
Martins
Maria Salete F
MS
Gomes-da-Silva
Maria Helena G
MH
D000273:Adipose Tissue / Q000378:metabolism; D000704:Analysis of Variance; D000818:Animals; D018753:Diet, Protein-Restricted / Q000009:adverse effects*; D005260:Female; D005333:Fetus / Q000378:metabolism; D006066:Gonads / Q000378:metabolism; D006728:Hormones / Q000096:biosynthesis; D008055:Lipids / Q000096:biosynthesis; D008099:Liver / Q000737:chemistry / Q000201:enzymology; D008112:Liver Glycogen / Q000096:biosynthesis; D008291:Malate Dehydrogenase / Q000096:biosynthesis; D008321:Mammary Glands, Animal / Q000378:metabolism; D039382:Maternal Nutritional Physiological Phenomena / Q000502:physiology*; D023421:Models, Animal; D010920:Placenta / Q000378:metabolism; D011247:Pregnancy; D051381:Rats; D017208:Rats, Wistar
D006728:Hormones; D008055:Lipids; D008112:Liver Glycogen; D008291:Malate Dehydrogenase