Effect of protocatechuic acid from Alpinia oxyphylla on proliferation of human adipose tissue-derived stromal cells in vitro.
The effect of protocatechuic acid (PCA) from Alpinia oxyphylla and catapol from Rehmannia on the proliferation capacity of human adipose tissue-derived stromal cells (hADSCs) was investigated in vitro. Cell counts showed that treatment of hADSCs with PCA for 48 h increased the cell number in a dose-dependent manner, while no obvious effect of catapol on the proliferation of hADSCs was observed. In addition, the cell number of hADSCs treated by 1.5 mM PCA increased in a time-dependent manner. The flow cytometric analysis of DNA content demonstrated the cell cycle progress from the G0/G1 phase to the S phase. Western blot analysis revealed the elevated expression of cyclin D1 in hADSCs induced by PCA treatment. Cyclin D1-siRNA transfection significantly inhibit the promotion of cell proliferation by PCA. Furthermore, the flow cytometric analysis of the cell surface antigens and the multidifferential potential tests of PCA-treated hADSCs showed that the cells retained their functional characteristics of multipotential mesenchymal progenitors. It is concluded that PCA can effectively up-regulate the proliferation of hADSCs.
Dalian R&D Center for Stem Cell and Tissue Engineering, Dalian University of Technology, Environmental and Life Science Building 3-403, No. 2 Linggong Road, Ganjingzi District, Dalian, 116024, China.
Han
H
Wang
Tian-Qing
TQ
Liu
Yan-Xia
YX
Zhu
Shui
S
Guan
Xue-Hu
XH
Ma
Zhan-Feng
ZF
Cui
D000273:Adipose Tissue / Q000166:cytology; D032429:Alpinia; D002453:Cell Cycle; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D019938:Cyclin D1 / Q000502:physiology; D004305:Dose-Response Relationship, Drug; D006801:Humans; D062385:Hydroxybenzoates / Q000494:pharmacology*; D007700:Kinetics; D059630:Mesenchymal Stem Cells; D039902:Multipotent Stem Cells; D000644:Quaternary Ammonium Compounds / Q000494:pharmacology; D032267:Rehmannia; D017154:Stromal Cells / Q000166:cytology / Q000187:drug effects*
D062385:Hydroxybenzoates; D000644:Quaternary Ammonium Compounds; D019938:Cyclin D1; C009091:protocatechuic acid; C031490:catamine AB