Distribution and rate of microbial processes in an ammonia-loaded air filter biofilm.
The in situ activity and distribution of heterotrophic and nitrifying bacteria and their potential interactions were investigated in a full-scale, two-section, trickling filter designed for biological degradation of volatile organics and NH(3) in ventilation air from pig farms. The filter biofilm was investigated by microsensor analysis, fluorescence in situ hybridization, quantitative PCR, and batch incubation activity measurements. In situ aerobic activity showed a significant decrease through the filter, while the distribution of ammonia-oxidizing bacteria (AOB) was highly skewed toward the filter outlet. Nitrite oxidation was not detected during most of the experimental period, and the AOB activity therefore resulted in NO(2)(-), accumulation, with concentrations often exceeding 100 mM at the filter inlet. The restriction of AOB to the outlet section of the filter was explained by both competition with heterotrophic bacteria for O(2) and inhibition by the protonated form of NO(2)(-), HNO(2). Product inhibition of AOB growth could explain why this type of filter tends to emit air with a rather constant NH(3) concentration irrespective of variations in inlet concentration and airflow.
Juhler
Susanne
S
Department of Biological Sciences, Aarhus University, Denmark. susanne.juhler@biology.au.dk
Revsbech
Niels Peter
NP
Schramm
Andreas
A
Herrmann
Martina
M
Ottosen
Lars D M
LD
Nielsen
Lars Peter
LP
D000388:Air; D000641:Ammonia / Q000378:metabolism*; D000818:Animals; D001419:Bacteria / Q000302:isolation & purification* / Q000378:metabolism*; D018441:Biofilms / Q000254:growth & development*; D005374:Filtration; D017403:In Situ Hybridization; D009573:Nitrites / Q000378:metabolism; D010084:Oxidation-Reduction; D016133:Polymerase Chain Reaction; D026361:Reactive Nitrogen Species / Q000378:metabolism; D013552:Swine; D055549:Volatile Organic Compounds / Q000378:metabolism
D009573:Nitrites; D026361:Reactive Nitrogen Species; D055549:Volatile Organic Compounds; D000641:Ammonia