Acute pancreatitis: hypertonic saline increases heat shock proteins 70 and 90 and reduces neutrophil infiltration in lung injury.
OBJECTIVE
Acute pancreatitis (AP) protease release induces lung parenchymal destruction via matrix metalloproteinases (MMPs), a neutrophil (polymorphonuclear leukocyte)-dependent process. Recent studies in hemorrhagic shock revealed that hypertonic saline (HTS) has an anti-inflammatory effect and can inhibit a variety of neutrophil functions. The aim of this study was to determine whether HTS and its actions in the pathway of neutrophil migration, MMPs, and heat shock proteins (HSPs) are effective in protecting the lung from injury associated with AP.


METHODS
We determined neutrophil infiltration and expressions of MMPs and HSPs in the lung tissue after AP induced by retrograde infusion of 2.5% of sodium taurocholate.


RESULTS
Animals submitted to AP that received HTS compared with those who received normal saline presented with increased HSP70 and HSP90 expressions and reduced myeloperoxidase levels and MMP-9 expression and activity.


CONCLUSIONS
Our data raised the hypothesis that a sequence of HTS lung protection events increases HSP70 and HSP90, inhibiting infiltration of neutrophils and their protease actions in the lung.
Moretti
Ana Iochabel Soares
AI
Emergency Medicine Department, Medical School, University of São Paulo, São Paulo, Brazil. aismoretti@yahoo.com.br
Rios
Ester Correia Sarmento
EC
Soriano
Francisco Garcia
FG
de Souza
Heraldo Possolo
HP
Abatepaulo
Fátima
F
Barbeiro
Denise Frediani
DF
Velasco
Irineu Tadeu
IT
D000208:Acute Disease; D000818:Animals; D015870:Gene Expression / Q000187:drug effects; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism*; D018841:HSP90 Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism*; D015151:Immunoblotting; D055370:Lung Injury / Q000209:etiology / Q000235:genetics / Q000378:metabolism*; D008297:Male; D020778:Matrix Metalloproteinase 2 / Q000235:genetics / Q000378:metabolism; D020780:Matrix Metalloproteinase 9 / Q000235:genetics / Q000378:metabolism; D020556:Neutrophil Infiltration / Q000187:drug effects*; D010195:Pancreatitis / Q000139:chemically induced / Q000150:complications*; D051381:Rats; D017208:Rats, Wistar; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012462:Saline Solution, Hypertonic / Q000494:pharmacology*; D013656:Taurocholic Acid
D018840:HSP70 Heat-Shock Proteins; D018841:HSP90 Heat-Shock Proteins; D012462:Saline Solution, Hypertonic; D013656:Taurocholic Acid; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9