Cytotoxic activity of cycloartane triterpenoids from Sphaerophysa salsula.
The cytotoxicity of three cycloartane triterpenoids, 9,19-cycloart-7beta, 24R, 25- triol-1-en-3-one (1), 9,19-cycloart-7beta, 24R, 25-triol-1-en-3-one 25-O-beta-D-glucopynanoside (2), and 25-O-beta-D-arabinopyranosyl-(1-->4)- beta-D-glucopyranosyl-9,19-cycloart-7beta, 24R, 25-triol-1-en-3-one (3), isolated from Sphaerophysa salsula was investigated on SF188, U87wt, MCF-7, and H460 cancer cell lines. Compound 1 showed the strongest activity. Cell cycle analysis was employed to elucidate the cytotoxicity on the tested U87wt cells, which led to G2/M arrest. In addition, from the Western blotting experiments, the expression of P21 is increased.
Wang
Dan
D
Zhejiang Institute for Drug Control, Hangzhou 310004, China.
Ma
Zhongjun
Z
D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry* / Q000494:pharmacology*; D002453:Cell Cycle / Q000187:drug effects; D045744:Cell Line, Tumor; D004305:Dose-Response Relationship, Drug; D007887:Fabaceae / Q000737:chemistry*; D006801:Humans; D015394:Molecular Structure; D014315:Triterpenes / Q000737:chemistry* / Q000494:pharmacology*
C541207:25-O-beta-D-arabinopyranosyl-(1-4)- beta-D-glucopyranosyl-9,19-cycloart-7beta, 24R, 25-triol-1-en-3-one; C541209:9,19-cycloart-7beta, 24R, 25-triol-1-en-3-one 25-O-beta-D-glucopynanoside; D000972:Antineoplastic Agents, Phytogenic; D014315:Triterpenes