Lingual antimicrobial peptide expresses in buffalo mammary gland.
Mammalian beta-defensins are innate host defense peptides with potential as a therapeutic agent. Present study reports expression of beta-defensin mRNA transcript in teat mucosal layer of Bubalus bubalis, which is homologous to lingual antimicrobial peptide (LAP) of cattle. The PCR-amplified and cloned full-length cDNA encodes for a putative peptide of 64 residues ( approximately 7 kDa) with pI of 11.324 and possess characteristic features of beta-defensin family including presence of six evolutionary conserved cysteine residues. The first 21 amino acids constitute a putative signal peptide, suggesting that it is a secretory protein. The peptide shares 85%-100% sequence identity with beta-defensins of cattle (Bos taurus). Nonetheless, the maximum identity at nucleotide (100%) and amino acid (98.5%) level was with cattle LAP, which is in consistent with phylogeny analysis report. Hence, the cloned beta-defensin has been designated as buffalo LAP. This study envisages role of beta-defensin peptides in innate immunity of mammary gland.
Das
Hemen
H
College of Veterinary Science and A. H., SDAU, Gujarat, India. hemenvet@rediffmail.com
Ahmed
Shahaj Uddin
SU
Thakuria
Dimpal
D
Pathan
M M
MM
Latif
A
A
Roy
S K
SK
More
Tukaram
T
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002020:Buffaloes / Q000235:genetics* / Q000276:immunology*; D003001:Cloning, Molecular; D017931:DNA Primers / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics; D005260:Female; D015870:Gene Expression; D007113:Immunity, Innate / Q000235:genetics; D008321:Mammary Glands, Animal / Q000276:immunology; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D023083:beta-Defensins / Q000235:genetics* / Q000276:immunology*
D017931:DNA Primers; D018076:DNA, Complementary; D012333:RNA, Messenger; D023083:beta-Defensins; C092480:lingual antimicrobial peptide