Resistin up-regulates fractalkine expression in human endothelial cells: lack of additive effect with TNF-alpha.
Resistin is a cytokine and fractalkine (Fk) a cell adhesion molecule and chemokine that contribute to human vascular inflammation by mechanisms not clearly defined. We questioned whether resistin induces Fk expression in human endothelial cells (HEC), compared the effect with that of the pro-inflammatory cytokine, TNF-alpha, and evaluated the consequences of co-stimulating HEC with both activators on Fk induction and on the signalling molecules involved. We found that resistin up-regulated Fk expression at comparable level to that of TNF-alpha by a mechanism involving P38 and JNK MAPK and NF-kappaB. Co-stimulation of cells with resistin and TNF-alpha did not increase Fk expression induced by every single inducer. Moreover resistin reduced the expression induced by TNF-alpha in HEC. The new data uncover Fk as a novel molecular link between resistin and inflammation and show that resistin and TNF-alpha have no additive effect in Fk up-regulation or on the signalling molecules implicated.
Institute of Cellular Biology and Pathology Nicolae Simionescu, 8 B.P. Hasdeu Street, P.O. Box 35-14, 050568 Bucharest, Romania. ileana.manduteanu@icbp.ro
Ileana
I
Manduteanu
Elena
E
Dragomir
Manuela
M
Calin
Monica
M
Pirvulescu
Ana Maria
AM
Gan
Daniela
D
Stan
Maya
M
Simionescu
D002460:Cell Line; D054428:Chemokine CX3CL1 / Q000096:biosynthesis*; D042783:Endothelial Cells / Q000187:drug effects / Q000378:metabolism*; D006801:Humans; D007249:Inflammation / Q000378:metabolism*; D048031:JNK Mitogen-Activated Protein Kinases / Q000378:metabolism; D016328:NF-kappa B / Q000378:metabolism; D047888:Receptors, Tumor Necrosis Factor, Type I / Q000037:antagonists & inhibitors; D052243:Resistin / Q000378:metabolism* / Q000494:pharmacology; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism* / Q000494:pharmacology; D015854:Up-Regulation; D048051:p38 Mitogen-Activated Protein Kinases / Q000378:metabolism
D054428:Chemokine CX3CL1; D016328:NF-kappa B; D047888:Receptors, Tumor Necrosis Factor, Type I; D052243:Resistin; D014409:Tumor Necrosis Factor-alpha; D048031:JNK Mitogen-Activated Protein Kinases; D048051:p38 Mitogen-Activated Protein Kinases