A role of miR-27 in the regulation of adipogenesis.
MicroRNAs (miRNAs) are involved in a plethora of important biological processes, from embryonic development to homeostasis in adult tissues. Recently, miRNAs have emerged as a class of epigenetic regulators of metabolism and energy homeostasis. We have investigated the role of miRNAs in the regulation of adipogenic differentiation. In this article, we demonstrate that the miR-27 gene family is downregulated during adipogenic differentiation. Overexpression of miR-27 specifically inhibited adipocyte formation, without affecting myogenic differentiation. We also found that expression of miR-27 resulted in blockade of expression of PPARgamma and C/EBPalpha, the two master regulators of adipogenesis. Importantly, expression of miR-27 was increased in fat tissue of obese mice and was regulated by hypoxia, an important extracellular stress associated with obesity. Our data strongly suggest that miR-27 represents a new class of adipogenic inhibitors and may play a role in the pathological development of obesity.
Lin
Qun
Q
Department of Therapeutic Radiology, Yale University School of Medicine, New Haven, CT 06510, USA.
Gao
Zhanguo
Z
Alarcon
Rodolfo M
RM
Ye
Jianping
J
Yun
Zhong
Z
D041721:3T3-L1 Cells; D050156:Adipogenesis / Q000235:genetics*; D000818:Animals; D002454:Cell Differentiation; D002478:Cells, Cultured; D051379:Mice; D008820:Mice, Obese; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D047495:PPAR gamma / Q000235:genetics / Q000378:metabolism; D014162:Transfection
D035683:MicroRNAs; C540674:Mirn27 microRNA, mouse; D047495:PPAR gamma