A library of mutated Maackia amurensis hemagglutinin distinguishes putative glycoforms of immunoglobulin A1 from IgA nephropathy patients.
Ten genetically modified Maackia amurensis hemagglutinin (MAH) clones at the carbohydrate-recognition loop were found to bind glycophorin A and a mucin mimetic with NeuAcalpha2-3Galbeta1-3GalNAcalpha (monosialyl-T antigen) in different relative intensity. Binding profiles of these lectins to human serum IgA1 from healthy individuals and from IgA nephropathy patients were subjected to the cluster analysis. Two large groups, one with only healthy individuals and another with all IgA nephropathy patients, were generated. The results strongly suggest that the library of genetically modified MAH is a useful tool for serum diagnosis of IgA nephropathy.
Laboratory of Cancer Biology and Molecular Immunology, Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.
Keisuke
K
Maenuma
Mijung
M
Yim
Kunimitsu
K
Komatsu
Mayumi
M
Hoshino
Ayumi
A
Tachiki-Fujioka
Kazuo
K
Takahashi
Yoshiyuki
Y
Hiki
Nicolai
N
Bovin
Tatsuro
T
Irimura
D000595:Amino Acid Sequence; D002241:Carbohydrates / Q000737:chemistry; D016000:Cluster Analysis; D004912:Erythrocytes / Q000378:metabolism*; D005922:Glomerulonephritis, IGA / Q000378:metabolism / Q000473:pathology*; D006023:Glycoproteins / Q000737:chemistry*; D006801:Humans; D007070:Immunoglobulin A / Q000097:blood / Q000235:genetics*; D037102:Lectins / Q000737:chemistry; D008969:Molecular Sequence Data; D009077:Mucins / Q000737:chemistry; D009154:Mutation; D010835:Phytohemagglutinins / Q000737:chemistry*; D011485:Protein Binding; D040901:Proteomics / Q000379:methods*
D002241:Carbohydrates; D006023:Glycoproteins; D007070:Immunoglobulin A; D037102:Lectins; D009077:Mucins; D010835:Phytohemagglutinins; C100156:leukoagglutinins, plants