[Visualization of viruses in living cells].
Live-cell imaging of cells infected with recombinant viruses expressing fluorescently tagged structural viral proteins enables to visualize virion maturation pathways at a virion particle level in the same cells as infection progresses. This technology have gradually unveiled previously unreported aspects of the pathways. This article focuses on live-cell imaging technology of herpes simplex virus 1 (HSV-1) and reviews the up-to-date topics of HSV-1 maturation pathway including our recent progress.
Kawaguchi
Yasushi
Y
Division of Viral Infection, Department of Infectious Disease Control, International Research Center for Infectious Diseases, Institute of Medical Science, The University of Tokyo, Tokyo, Japan. ykawagu@ims.u-tokyo.ac.jp
D000818:Animals; D002522:Chlorocebus aethiops; D005456:Fluorescent Dyes; D018259:Herpesvirus 1, Human / Q000502:physiology* / Q000648:ultrastructure*; D006801:Humans; D018613:Microscopy, Confocal; D014709:Vero Cells / Q000821:virology*; D014771:Virion / Q000502:physiology / Q000648:ultrastructure; D014779:Virus Replication
D005456:Fluorescent Dyes