Angiotensin-(1-7) stimulates hematopoietic progenitor cells in vitro and in vivo.
Effects of angiotensin (Ang)-(1-7), an AngII metabolite, on bone marrow-derived hematopoietic cells were studied. We identified Ang-(1-7) to stimulate proliferation of human CD34(+) and mononuclear cells in vitro. Under in vivo conditions, we monitored proliferation and differentiation of human cord blood mononuclear cells in NOD/SCID mice. Ang-(1-7) stimulated differentially human cells in bone marrow and accumulated them in the spleen. The number of HLA-I(+) and CD34(+) cells in the bone marrow was increased 42-fold and 600-fold, respectively. These results indicate a decisive impact of Ang-(1-7) on hematopoiesis and its promising therapeutic potential in diseases requiring progenitor stimulation.
1Department of Gynaecology, University of Leipzig, Germany.
Silvia
S
Heringer-Walther
Klaus
K
Eckert
Sarah-Mai
SM
Schumacher
Lutz
L
Uharek
Annika
A
Wulf-Goldenberg
Florian
F
Gembardt
Iduna
I
Fichtner
Heinz-Peter
HP
Schultheiss
Kathy
K
Rodgers
Thomas
T
Walther
D000803:Angiotensin I / Q000008:administration & dosage / Q000494:pharmacology*; D000818:Animals; D018941:Antigens, CD19 / Q000032:analysis; D018952:Antigens, CD34 / Q000032:analysis; D001854:Bone Marrow Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D040881:CD11a Antigen / Q000032:analysis; D002478:Cells, Cultured; D036101:Cord Blood Stem Cell Transplantation / Q000379:methods; D004305:Dose-Response Relationship, Drug; D005312:Fetal Blood / Q000166:cytology / Q000378:metabolism; D005434:Flow Cytometry; D006684:HLA-DR Antigens / Q000032:analysis; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D006801:Humans; D007279:Injections, Subcutaneous; D007963:Leukocytes, Mononuclear / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D016256:Lewis X Antigen / Q000032:analysis; D051379:Mice; D016688:Mice, Inbred NOD; D016513:Mice, SCID; D010446:Peptide Fragments / Q000008:administration & dosage / Q000494:pharmacology*; D013154:Spleen / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D013997:Time Factors; D014183:Transplantation, Heterologous
D018941:Antigens, CD19; D018952:Antigens, CD34; D040881:CD11a Antigen; D006684:HLA-DR Antigens; D016256:Lewis X Antigen; D010446:Peptide Fragments; D000803:Angiotensin I; C118790:angiotensin I (1-7)