Involvement of PTP-RQ in differentiation during adipogenesis of human mesenchymal stem cells.
Mesenchymal stem cells (MSCs) are self-renewable multipotent progenitor cells with the capacity to differentiate into several distinct mesenchymal lineages. While MSCs display significant potential in tissue engineering and therapeutic applications, the regulatory mechanisms underlying the differentiation of these cells are yet to be established. Phosphorylation is a post-translational modification that plays a significant role in diverse biological phenomena. In this study, to mine the protein tyrosine phosphatases (PTPs) involved in adipogenesis of human MSCs, differential expression of human PTPs was examined using RT-PCR analysis. Among the 107 human PTPs, PTP-RQ was dramatically downregulated during the early phase of adipogenesis. PTP-RQ is classified as a receptor-type III PTP with phosphatidylinositol phosphatase (PIPase) activity. Overexpression of PTP-RQ consistently led to reduced differentiation of MSCs into adipocytes via decreasing the phosphatidyl inositol phosphate level in cells, and consequently downregulating Akt/PKB phosphorylation. Our results collectively suggest that PTP-RQ is a useful target protein for regulating the differentiation of MSCs into adipocytes, and may be used to develop novel drugs for the treatment of obesity.
Jung
Hyeyun
H
Medical Proteomics Research Center, KRIBB, Daejeon, Republic of Korea.
Kim
Won Kon
WK
Kim
Do Hyung
DH
Cho
Yee Sook
YS
Kim
Seung Jun
SJ
Park
Sung Goo
SG
Park
Byoung Chul
BC
Lim
Heon Man
HM
Bae
Kwang-Hee
KH
Lee
Sang Chul
SC
D050156:Adipogenesis / Q000235:genetics; D002478:Cells, Cultured; D015536:Down-Regulation; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000201:enzymology; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D054631:Receptor-Like Protein Tyrosine Phosphatases, Class 3 / Q000235:genetics / Q000378:metabolism*
D051057:Proto-Oncogene Proteins c-akt; C516917:PTPRQ protein, human; D054631:Receptor-Like Protein Tyrosine Phosphatases, Class 3