In vitro selection of protein and peptide libraries using mRNA display.
In vitro genetic approaches are powerful solutions to the protein design problem. mRNA display is an in vitro selection technique enabling the design of peptide and protein ligands ranging from one to over 100 amino acids. Libraries containing more than 10 trillion unique sequences can be synthesized and sieved with exquisite control over binding stringency and specificity.
Takahashi
Terry T
TT
Department of Chemistry, University of Southern California, Los Angeles, CA, USA.
Roberts
Richard W
RW
D020650:Combinatorial Chemistry Techniques; D008024:Ligands; D019151:Peptide Library; D010455:Peptides / Q000737:chemistry* / Q000235:genetics* / Q000378:metabolism; D011485:Protein Binding; D015202:Protein Engineering; D012333:RNA, Messenger / Q000737:chemistry* / Q000235:genetics* / Q000378:metabolism; D052156:SELEX Aptamer Technique / Q000379:methods*
D008024:Ligands; D019151:Peptide Library; D010455:Peptides; D012333:RNA, Messenger