Fabrication of orderly nanostructured PLGA scaffolds using anodic aluminum oxide templates.
In this research, two simple fabrication methods to fabricate orderly nanostructured PLGA scaffolds using anodic aluminum oxide (AAO) template were conducted. In the vacuum air-extraction approach, the PLGA solution was cast on an AAO template first. The vacuum air-extraction process was then applied to suck the semi-congealed PLGA into the nanopores of the AAO template to form a bamboo sprouts array of PLGA. The surface roughness of the nanostructured scaffolds, ranging from 20 nm to 76 nm, can be controlled by the sucking time of the vacuum air-extraction process. In the replica molding approach, the PLGA solution was cast on the orderly scraggy barrier-layer surface of an AAO membrane to fabricate a PLGA scaffold of concave nanostructure. Cell culture experiments using the bovine endothelial cells (BEC) demonstrated that the nanostructured PLGA membrane can increase the cell growing rate, especially for the bamboo sprouts array scaffolds with smaller surface roughness.
Department of Mechanical Engineering, National Chung-Hsing University, Taichung 40227, Taiwan. gjwang@dragon.nchu.edu.tw
Gou-Jen
GJ
Wang
Yan-Cheng
YC
Lin
Ching-Wen
CW
Li
Cheng-Chih
CC
Hsueh
Shan-Hui
SH
Hsu
Huey-Shan
HS
Hung
D000537:Aluminum Oxide / Q000737:chemistry*; D000818:Animals; D002417:Cattle; D018929:Cell Culture Techniques; D002478:Cells, Cultured; D042783:Endothelial Cells / Q000166:cytology*; D019344:Lactic Acid / Q000737:chemistry*; D049329:Nanostructures / Q000737:chemistry*; D011100:Polyglycolic Acid / Q000737:chemistry*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D016062:Porosity
D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; D000537:Aluminum Oxide