Hydrogen-rich saline protects against intestinal ischemia/reperfusion injury in rats.
Hydrogen gas was reported to reduce reactive oxygen species and alleviate cerebral, myocardial and hepatic ischemia/reperfusion (I/R) injuries. This paper studied the effect of hydrogen-rich saline, which was easier for clinical application, on the intestinal I/R injury. Model of intestinal I/R injury was induced in male Sprague-Dawley rats. Physiological saline, hydrogen-rich saline or nitrogen-rich saline (5 ml/kg) was administered via intravenous infusion at 10 min before reperfusion, respectively. The intestine damage was detected microscopically and was assessed by Chiu score system after I/R injury. In addition, serum DAO activity, TNF-alpha, IL-1beta and IL-6 levels, tissue MDA, protein carbonyl and MPO activity were all increased significantly by I/R injury. Hydrogen-rich saline reduced these markers and relieved morphological intestinal injury, while no significant reduction was observed in the nitrogen-rich saline-treated animals. In conclusion, hydrogen-rich saline protected the small intestine against I/R injury, possibly by reduction of inflammation and oxidative stress.
Chinese PLA Institute of Burn Surgery & Department of Burn Surgery, Changhai Hospital, Second Military Medical University, Shanghai, PR China.
Xingfeng
X
Zheng
Yanfei
Y
Mao
Jianmei
J
Cai
Yonghua
Y
Li
Wenwu
W
Liu
Pengling
P
Sun
John H
JH
Zhang
Xuejun
X
Sun
Hongbin
H
Yuan
D000818:Animals; D016207:Cytokines / Q000097:blood; D006859:Hydrogen / Q000008:administration & dosage*; D007249:Inflammation / Q000517:prevention & control; D018836:Inflammation Mediators / Q000097:blood; D007262:Infusions, Intravenous; D007413:Intestinal Mucosa / Q000378:metabolism; D007422:Intestines / Q000098:blood supply* / Q000293:injuries* / Q000473:pathology; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D009504:Neutrophils / Q000187:drug effects / Q000473:pathology; D009584:Nitrogen / Q000008:administration & dosage; D018384:Oxidative Stress / Q000187:drug effects; D009195:Peroxidase / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*; D012965:Sodium Chloride / Q000008:administration & dosage
D016207:Cytokines; D018836:Inflammation Mediators; D012965:Sodium Chloride; D008315:Malondialdehyde; D006859:Hydrogen; D009195:Peroxidase; D009584:Nitrogen