Gene Context and DNA rearrangements in the carbapenemase locus of division II strains of Bacteroides fragilis.
The cfiA gene is clustered in a bicistronic operon encoding an N-acetyltransferase and an O-acetyltransferase related to resistance markers. This genetic context, exclusively found in strains of Bacteroides fragilis division II, has been highly rearranged by the successive integration of two new mobile sequences, a miniature element and ISBf9. Besides that, among the DNA polymorphisms detected in the cfiA locus, only the integration of IS942 at its promoter was a determinant for expression of carbapenemase activity.
Departamento de Bioquímica, Facultad de Veterinaria, Universidad de Extremadura, Avenida de la Universidad s/n, 10071-Cáceres, Spain.
Nuria
N
García
Gloria
G
Gutiérrez
María
M
Lorenzo
Santiago
S
Vadillo
Segundo
S
Píriz
Alberto
A
Quesada
D000483:Alleles; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D001441:Bacteroides fragilis / Q000235:genetics*; D001483:Base Sequence; D015321:Gene Rearrangement; D008969:Molecular Sequence Data; D011110:Polymorphism, Genetic; D001618:beta-Lactamases / Q000235:genetics* / Q000378:metabolism
D001426:Bacterial Proteins; D001618:beta-Lactamases; C063912:carbapenemase