Surface modifications of photocrosslinked biodegradable elastomers and their influence on smooth muscle cell adhesion and proliferation.
Photocrosslinked, biodegradable elastomers based on aliphatic polyesters have many desirable features as scaffolds for smooth muscle tissue engineering. However, they lack cell adhesion motifs. To address this shortcoming, two different modification procedures were studied utilizing a high and a low crosslink density elastomer: base etching and the incorporation of acryloyl-poly(ethylene glycol) (PEG)-Gly-Arg-Gly-Asp-Ser (GRGDS) into the elastomer network during photocrosslinking. Base etching improved surface hydrophilicity without altering surface topography, but did not improve bovine aortic smooth muscle cell adhesion. Incorporation of PEG-GRGDS into the elastomer network significantly improved cell adhesion for both high and low crosslink density elastomers, with a greater effect with the higher crosslink density elastomer. Incorporation of GRGDS into the high crosslink density elastomer also enhanced smooth muscle cell proliferation, while proliferation on the low crosslink density unmodified, base etched, and PEG-GRGDS incorporated elastomers was significantly greater than on the high crosslink density unmodified and base etched elastomer.
Ilagan
Bernadette G
BG
Department of Chemical Engineering and Human Mobility Research Centre, Queen's University, Kingston, Ont., Canada K7L 3N6.
Amsden
Brian G
BG
D020341:Absorbable Implants; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry* / Q000528:radiation effects; D040761:Biomimetic Materials / Q000737:chemistry; D002417:Cattle; D002448:Cell Adhesion; D018929:Cell Culture Techniques / Q000379:methods; D049109:Cell Proliferation; D003432:Cross-Linking Reagents / Q000737:chemistry / Q000528:radiation effects; D020724:Elastomers / Q000737:chemistry* / Q000528:radiation effects; D005109:Extracellular Matrix / Q000737:chemistry; D008422:Materials Testing; D032389:Myocytes, Smooth Muscle / Q000166:cytology* / Q000502:physiology*; D013499:Surface Properties / Q000528:radiation effects; D023822:Tissue Engineering / Q000379:methods*
D001672:Biocompatible Materials; D003432:Cross-Linking Reagents; D020724:Elastomers