Simultaneous quantification of seven major bufadienolides in three traditional Chinese medicinal preparations of chansu by HPLC-DAD.
A high-performance liquid chromatographic method was applied to the determination of gamabufotalin, telocinobufagin, bufotalin, cinobufotalin, bufalin, cinobufagin and resibufogenin in three traditional Chinese medicinal preparations containing ChanSu. The compounds were separated on a YMC-C18 column (250 x 4.6 mm, 5 um) with a gradient of acetonitrile and 0.3% aqueous acetic acid (v/v) at a flow rate of 0.8 mL min(-1) and detected at 296 nm. Complete separation was obtained within 35 min for the seven bufadienolides. The calibration curves showed good linearity (r2 > 0.999) within the test range. The recovery was 95.5% - 105.9%. The assay could simultaneously determine seven major bufadienolides of the three Chinese medicinal preparations of ChanSu in 35 min. The results obtained suggested that the developed HPLC assay could be comprehensively utilized for the quality control of the three traditional Chinese medicinal preparations of ChanSu used in the clinic.
School of Pharmaceutical Sciences, Dalian Medical University, Dalian 116044, PR China. maxc1978@sohu.com
Xiao Chi
XC
Ma
Bao Jing
BJ
Zhang
Xin Lan
XL
Xin
Shan Shan
SS
Huang
Sha
S
Deng
Hou Li
HL
Zhang
Xiao Hong
XH
Shu
Yun Peng
YP
Diao
Jian
J
Cui
D002018:Bufanolides / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D008516:Medicine, Chinese Traditional; D015394:Molecular Structure
D002018:Bufanolides; D004365:Drugs, Chinese Herbal; C095591:chan su