Preparation and time-gated luminescence bioimaging application of ruthenium complex covalently bound silica nanoparticles.
Luminescent ruthenium(II) complex covalently bound silica nanoparticles have been prepared and used as a probe for time-gated luminescence bioimaging. The new nanoparticles were prepared by copolymerization of a luminescent Ru(II) complex tris(5-amino-1,10-phenanthroline)ruthenium(II) conjugated with 3-aminopropyl(triethoxy)silane (APS-Ru conjugate), free (3-aminopropyl)triethoxysilane (APS) and tetraethyl orthosilicate (TEOS) in a water-in-oil reverse microemulsion consisting of Triton X-100, n-octanol, cyclohexane and water in the presence of aqueous ammonia. Characterization by transmission electron microscopy indicates that the nanoparticles are monodisperse, spherical and uniform in size, 64+/-4 nm in diameter. Compared with the dye-doping nanoparticles, dye leakage of the new nanoparticles was remarkably decreased. In addition, it was found that the Ru(II) complex luminescence could be effectively enhanced with a longer luminescence lifetime (approximately 2.3 micros) after forming the nanoparticles, which enables the nanoparticles to be suitable as a bioprobe for time-gated luminescence bioimaging applications. The nanoparticle-labeled streptavidin was prepared and successfully used for time-gated luminescence imaging detection of an environmental pathogen, Giardia lamblia, with high specificity and sensitivity.
Song
Cuihong
C
Department of Instrumentation & Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, PR China.
Ye
Zhiqiang
Z
Wang
Guilan
G
Jin
Dayong
D
Yuan
Jingli
J
Guan
Yafeng
Y
Piper
James
J
D000818:Animals; D003952:Diagnostic Imaging / Q000379:methods* / Q000592:standards; D016829:Giardia lamblia / Q000302:isolation & purification; D005873:Giardiasis / Q000175:diagnosis; D008163:Luminescent Measurements / Q000379:methods* / Q000592:standards; D053758:Nanoparticles / Q000737:chemistry; D012428:Ruthenium / Q000737:chemistry; D012822:Silicon Dioxide / Q000737:chemistry
D012822:Silicon Dioxide; D012428:Ruthenium