Molecular and functional characterization of adipokinetic hormone receptor and its peptide ligands in Bombyx mori.
Neuropeptides of the adipokinetic hormone (AKH) family are among the best studied hormone peptides, but its signaling pathways remain to be elucidated. In this study, we molecularly characterized the signaling of Bombyx AKH receptor (AKHR) and its peptide ligands in HEK293 cells. In HEK293 cells stably expressing AKHR, AKH1 stimulation not only led to a ligand concentration dependent mobilization of intracellular Ca(2+) and cAMP accumulation, but also elicited transient activation of extracellular signal-regulated kinase 1/2 (ERK1/2) pathway. We observed that AKH receptor was rapidly internalized after AKH1 stimulation. We further demonstrated that AKH2 exhibited high activities in cAMP accumulation and ERK1/2 activation on AKHR comparable to AKH1, whereas AKH3 was much less effective.
Zhu
Chenggang
C
Institute of Biochemistry, College of Life Sciences, Zijingang Campus, Zhejiang University, Hangzhou, Zhejiang 310058, China.
Huang
Haishan
H
Hua
Rongsheng
R
Li
Guo
G
Yang
Dong
D
Luo
Jiansong
J
Zhang
Cunxin
C
Shi
Liangen
L
Benovic
Jeffrey L
JL
Zhou
Naiming
N
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D012831:Bombyx / Q000378:metabolism*; D002118:Calcium / Q000378:metabolism; D002460:Cell Line; D000242:Cyclic AMP / Q000378:metabolism; D017931:DNA Primers; D005434:Flow Cytometry; D006801:Humans; D007301:Insect Hormones / Q000737:chemistry / Q000378:metabolism*; D008024:Ligands; D020935:MAP Kinase Signaling System; D008856:Microscopy, Fluorescence; D008969:Molecular Sequence Data; D009842:Oligopeptides / Q000737:chemistry / Q000378:metabolism*; D010446:Peptide Fragments / Q000378:metabolism*; D011761:Pyrrolidonecarboxylic Acid / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism; D018027:Receptors, Glucagon / Q000737:chemistry / Q000378:metabolism*
D017931:DNA Primers; D007301:Insect Hormones; D008024:Ligands; D009842:Oligopeptides; D010446:Peptide Fragments; D018027:Receptors, Glucagon; C044833:adipokinetic hormone; D000242:Cyclic AMP; D002118:Calcium; D011761:Pyrrolidonecarboxylic Acid