Poly-silicon nanowire field-effect transistor for ultrasensitive and label-free detection of pathogenic avian influenza DNA.
Enhanced surveillance of influenza requires rapid, robust, and inexpensive analytical techniques capable of providing a detailed analysis of influenza virus strains. Functionalized poly-crystalline silicon nanowire field-effect transistor (poly-SiNW FET) was demonstrated to achieve specific and ultrasensitive (at fM level) detection of high pathogenic strain virus (H5 and H7) DNA of avian influenza (AI) which is an important infectious disease and has an immediate need for surveillance. The poly-SiNW FET was prepared by a simple and low-cost method that is compatible with current commercial semiconductor process without expensive E-beam lithography tools for large-scale production. Specific electric changes were observed for AI virus DNA sensing when nanowire surface of poly-SiNW FET was modified with complementary captured DNA probe and target DNA (H5) at fM to pM range could be distinguished. With its excellent electric properties and potential for mass commercial production, poly-SiNW FET can be developed to become a portable biosensor for field use and point-of-care diagnoses.
Lin
Chih-Heng
CH
Institute of Biological Science and Technology, National Chiao Tung University, Hsinchu 300, Taiwan.
Hung
Cheng-Hsiung
CH
Hsiao
Cheng-Yun
CY
Lin
Horng-Chih
HC
Ko
Fu-Hsiang
FH
Yang
Yuh-Shyong
YS
D000818:Animals; D001483:Base Sequence; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods / Q000706:statistics & numerical data; D001717:Birds; D015342:DNA Probes / Q000235:genetics; D004279:DNA, Viral / Q000032:analysis* / Q000235:genetics; D055664:Electrochemical Techniques; D006801:Humans; D005585:Influenza in Birds / Q000175:diagnosis; D029521:Alphainfluenzavirus / Q000235:genetics / Q000302:isolation & purification* / Q000472:pathogenicity; D053770:Nanowires; D011159:Population Surveillance / Q000379:methods; D012680:Sensitivity and Specificity; D012825:Silicon; D014173:Transistors, Electronic
D015342:DNA Probes; D004279:DNA, Viral; D012825:Silicon