Direct electrochemistry of hemoglobin entrapped in cyanoethyl cellulose film and its electrocatalysis to nitric oxide.
A water-soluble cyanoethyl cellulose (CEC), homogeneously synthesized in NaOH/urea aqueous solution, was used as an immobilization matrix to entrap proteins and enzymes. Then hemoglobin (Hb) was used as a template to fabricate CEC-Hb biomimetic membranes in which the Hb showed direct electrochemistry on a glass carbon electrode (GCE). The characterizations of CEC-Hb film were demonstrated by ultraviolet-visible (UV-vis) spectra, scanning electron microscopy (SEM), and electrochemical impedance spectroscopy (EIS). The electrochemical behaviors of Hb in CEC film have been investigated and a pair of well-defined and quasi-reversible cyclic voltammetric peaks for the protein heme Fe(III)/Fe(II) redox couples was observed at about -0.369 V (vs. SCE). The CEC-Hb film exhibited a good electrocatalytic activity for the reduction of nitric oxide (NO). The amperometric response of the biosensor varied linearly with the NO concentration ranging from 1.1x10(-6) to 1.3x10(-4) mol L(-1). Moreover, the studied biosensor exhibited high sensibility, good reproducibility, and long-term stability. Finally, this method has applied to monitoring the NO release from biologic samples.
Jia
Shanshan
S
Key Laboratory of Environmentally Friendly Chemistry and Applications of Ministry of Education, Xiangtan University, Xiangtan 411105, China.
Fei
Junjie
J
Zhou
Jinping
J
Chen
Xiaoming
X
Meng
Jianqiang
J
D000818:Animals; D015374:Biosensing Techniques / Q000379:methods* / Q000706:statistics & numerical data; D002384:Catalysis; D002482:Cellulose / Q000031:analogs & derivatives; D055664:Electrochemical Techniques; D006454:Hemoglobins / Q000737:chemistry*; D009569:Nitric Oxide / Q000032:analysis*; D010084:Oxidation-Reduction; D051381:Rats; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D006454:Hemoglobins; D009569:Nitric Oxide; D002482:Cellulose