The WAVE regulatory complex is inhibited.
The WAVE regulatory complex (WRC) transmits information from the Rac GTPase to the actin nucleator Arp2/3 complex. We have reconstituted recombinant human and Drosophila WRC in several forms and shown that they are inactive toward Arp2/3 complex but can be activated by Rac in a nucleotide-dependent fashion. Our observations identify core components needed for WAVE inhibition, reconcile contradictory existing mechanisms and reveal common regulatory principles for the WAVE/WASP family of proteins.
Ismail
Ayman M
AM
Howard Hughes Medical Institute and Department of Biochemistry, University of Texas Southwestern Medical Center, Dallas, USA.
Padrick
Shae B
SB
Chen
Baoyu
B
Umetani
Junko
J
Rosen
Michael K
MK
D000818:Animals; D004331:Drosophila melanogaster / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D011994:Recombinant Proteins / Q000378:metabolism; D051300:Wiskott-Aldrich Syndrome Protein Family / Q000037:antagonists & inhibitors*
D011994:Recombinant Proteins; D051300:Wiskott-Aldrich Syndrome Protein Family