[Cloning and prokaryotic expression of lipid storage droplet protein 5 gene].
OBJECTIVE
To clone the Lipid storage droplet protein 5 (LSDP5) and express it in E.coli.


METHODS
LSDP5 gene was amplified by PCR from the mouse liver cDNA library, and inserted into pMD18-T vector and sequenced. After analysis of hydrophobicity and secondary structure using software, a fragment of LSDP5 was subcloned into GST fusion expression vector, and the recombinant expression plasmid pEGX-LSDP5 was constructed and expressed in E.coli BL21.


RESULTS
LSDP5 gene was obtained and its sequence was identical with that reported in GenBank. The E.coli containing pEGX-LSDP5 could express a 40 kD protein after induction of IPTG.


CONCLUSIONS
A confirmed LSDP5 gene is cloned and expressed in E.coli successfully, which lay the foundation for the related research of this gene.
Department of Pathology, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China. liqing@fmmu.edu.cn
Fang
F
Liu
Qing
Q
Li
Jing
J
Ye
Jing
J
Zhang
Li-ying
LY
Zhang
Fan-fan
FF
Li
Hang
H
Li
Yu
Y
Gu
D000818:Animals; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics*; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000235:genetics; D015870:Gene Expression; D057927:Hydrophobic and Hydrophilic Interactions; D008099:Liver / Q000378:metabolism; D051379:Mice; D000070797:Perilipin-5; D011506:Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism*; D017422:Sequence Analysis, DNA
D018076:DNA, Complementary; D000070797:Perilipin-5; C511805:Plin5 protein, mouse; D011506:Proteins; D011994:Recombinant Proteins