Electrical impedance measurements predict cellular transformation.
Cellular transformation is the first step in cancer development. Two features of cellular transformation are proliferation in reduced serum and loss of contact inhibition. Electronic Cell-Substrate Impedance Sensing (ECIS) measurements have been used to measure cellular proliferation, cytotoxicity, apoptosis, and attachment. We have used impedance measurements to distinguish normal cells from cells transformed with a constitutively active chemokine receptor, CXCR2. CXCR2, a member of the G-protein coupled receptor (GPCR) family, is normally involved in cellular activation and migration, but a single amino acid substitution leads to constitutive activity. NIH3T3 cells were transformed with a constitutively active CXCR2 (D143V_CXCR2) and growth in reduced serum and foci formation were measured using established biological assays and compared to data from ECIS. The results of this study show that impedance measurements provide a quick and reliable way of measuring cellular transformation and provide real time assessment of transformed cellular parameters. Use of the ECIS system could allow a rapid screening of anti-cancer drugs that alter cellular transformation.
The University of Tennessee, Department of Microbiology, Knoxville, 37996, USA.
Giljun
G
Park
Chang K
CK
Choi
Anthony E
AE
English
Tim E
TE
Sparer
D019943:Amino Acid Substitution; D000818:Animals; D015374:Biosensing Techniques; D002460:Cell Line; D002471:Cell Transformation, Neoplastic / Q000378:metabolism*; D017097:Electric Impedance; D055664:Electrochemical Techniques; D051379:Mice; D041681:NIH 3T3 Cells; D011237:Predictive Value of Tests; D023063:Receptors, Interleukin-8B / Q000096:biosynthesis / Q000378:metabolism; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000378:metabolism; D014162:Transfection
D023063:Receptors, Interleukin-8B; D011994:Recombinant Proteins