Intermittent microwave irradiation facilitates antigen-antibody reaction in Western blot analysis.
We established a shortened protocol for western blot analysis using intermittent microwave irradiation. With this method, the procedure is completed within 1 h after applying the primary antibody, and thus greatly saves time. This procedure appears to be applicable to any antibody based on our experience of several years.
Department of Pathology and Biology of Diseases, Graduate School of Medicine, Kyoto University, Yoshida-Konoe-cho, Sakyo-ku, Kyoto, 606-8501, Japan.
Yu-Ting
YT
Liu
Shinya
S
Toyokuni
D000818:Animals; D000937:Antigen-Antibody Reactions / Q000528:radiation effects*; D015153:Blotting, Western / Q000379:methods*; D008297:Male; D008872:Microwaves; D051381:Rats; D017208:Rats, Wistar