Comparison of culture techniques at different stages of brucellosis.
The lysis centrifugation technique is preferred for culturing Brucella spp. at all stages of brucellosis because it yields 25% more positive results and on average provides results 10 days earlier than the Ruiz-Castaneda method. This lysis method is inexpensive and easier to use and may be used in laboratories with limited expertise or equipment if all safety precautions are taken.
Naval Medical Research Center Detachment, Lima, Peru. benjamin.espinosa@med.navy.mil
Benjamin J
BJ
Espinosa
Jesús
J
Chacaltana
Maximilian
M
Mulder
María Pía
MP
Franco
David L
DL
Blazes
Robert H
RH
Gilman
Henk L
HL
Smits
Eric R
ER
Hall
D001431:Bacteriological Techniques / Q000379:methods*; D002002:Brucella / Q000302:isolation & purification*; D002006:Brucellosis / Q000175:diagnosis / Q000382:microbiology*; D006801:Humans; D011237:Predictive Value of Tests; D012680:Sensitivity and Specificity; D012698:Serologic Tests