Structure of a polysaccharide from the lipopolysaccharide of Vibrio vulnificus clinical isolate YJ016 containing 2-acetimidoylamino-2-deoxy-L-galacturonic acid.
A polysaccharide isolated after mild acid degradation of the lipopolysaccharide of Vibrio vulnificus clinical isolate YJ016 was found to contain L-Fuc, D-GlcpNAc, 2,4-diacetamido-2,4,6-trideoxy-D-glucose (di-N-acetylbacillosamine, D-QuiNAc4NAc), and 2-acetimidoylamino-2-deoxy-L-galacturonic acid (L-GalNAmA). The last sugar derivative was confirmed by correlations for nitrogen-linked protons in 2D TOCSY and ROESY spectra measured in a H(2)O-D(2)O mixture. The following structure of the polysaccharide was established by (1)H and (13)C NMR spectroscopy, including 2D ROESY and (1)H,(13)C HMBC experiments: [structure: see text], where the degree of 6-O-acetylation of the lateral beta-GlcNAc residue is approximately 70%.
N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia.
Sof'ya N
SN
Senchenkova
Alexander S
AS
Shashkov
Yuriy A
YA
Knirel
Consuelo
C
Esteve
Elena
E
Alcaide
Susana
S
Merino
Juan M
JM
Tomás
D002240:Carbohydrate Sequence; D008070:Lipopolysaccharides / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D011135:Polysaccharides, Bacterial / Q000737:chemistry*; D041261:Vibrio vulnificus / Q000737:chemistry*
D008070:Lipopolysaccharides; D011135:Polysaccharides, Bacterial