The antibacterial activity of Magainin I immobilized onto mixed thiols Self-Assembled Monolayers.
An antibacterial peptide, Magainin I, was covalently bound to a mixed 11-mercaptoundecanoïc acid (MUA) and 6-mercaptohexanol (C6OH) (ratio 1:3) Self-Assembled Monolayer (SAM) on gold surfaces. Each step of the surface functionalization was characterized by Polarization Modulation Reflection Absorption InfraRed Spectroscopy (PM-RAIRS) and X-ray Photoelectron Spectroscopy (XPS). The antibacterial activity of the anchored Magainin was tested against three Gram-positive bacteria (Listeria ivanovii, Enterococcus faecalis and Staphylococcus aureus), and the results revealed that the adsorbed Magainin I reduced by more than 50% the adhesion of bacteria at the surface, together with the killing of the bacteria that nonetheless adhered to the surface. No release of the peptide was observed upon contact with the bacterial suspension; the activity has proven to be persistent overtime, up to six months after the first use.
CNRS, UMR CNRS 7197, Laboratoire de Réactivité de Surface, 4 Place Jussieu, 75252 Paris Cedex 05, France. vincent.humblot@upmc.fr
Vincent
V
Humblot
Jean-Fabrice
JF
Yala
Pascal
P
Thebault
Kada
K
Boukerma
Arnaud
A
Héquet
Jean-Marc
JM
Berjeaud
Claire-Marie
CM
Pradier
D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000494:pharmacology; D023181:Antimicrobial Cationic Peptides / Q000737:chemistry* / Q000494:pharmacology; D005227:Fatty Acids / Q000737:chemistry*; D006046:Gold / Q000737:chemistry; D006094:Gram-Positive Bacteria / Q000187:drug effects; D008087:Listeria / Q000187:drug effects; D008422:Materials Testing; D008962:Models, Theoretical; D013055:Spectrophotometry, Infrared; D013211:Staphylococcus aureus / Q000187:drug effects; D013438:Sulfhydryl Compounds / Q000737:chemistry*; D029867:Xenopus Proteins / Q000737:chemistry* / Q000494:pharmacology
C505222:11-mercaptoundecanoic acid; D000900:Anti-Bacterial Agents; D023181:Antimicrobial Cationic Peptides; D005227:Fatty Acids; D013438:Sulfhydryl Compounds; D029867:Xenopus Proteins; C053300:magainin 1 peptide, Xenopus; D006046:Gold