[CpG-DNA inhibits cell reactions accompanied with the development of the adaptive response in human lymphocytes after low-dose X-ray exposure].
The lymphocytes of peripheral blood of healthy donors were influenced by X-ray radiation (10 cGy) or a fragments of the transcribed region of rDNA (TRrDNA) transmitted to the incubation medium of non-irradiated cells. Both factors induced transposition of the loci 1q12 of homologous chromosomes from the membrane to the centre of the nucleus in lymphocytes; produced the activation of the genes TLR9 and MyD88 expression, the chromosomal nucleolus-forming regions, TNF-alpha and caspase-3; and also increased nuclease activity and synthesis RNA of the cells. However all the investigated reaction in the cells did not developed during the synergetic radiation and TRrDNA but the activity level of the cytokine TNF-alpha was increasing. The reactions of human lymphocytes on the induced influence are discussed herein.
Ermakov
A V
AV
Kon'kova
M S
MS
Kostiuk
S V
SV
Kalashnikova
E A
EA
Kokarovtseva
S N
SN
Egolina
N A
NA
Veĭko
N N
NN
D000222:Adaptation, Physiological; D053148:Caspase 3 / Q000378:metabolism; D002877:Chromosomes, Human / Q000235:genetics / Q000528:radiation effects*; D018899:CpG Islands / Q000502:physiology*; D004247:DNA / Q000737:chemistry* / Q000494:pharmacology; D004275:DNA, Ribosomal / Q000378:metabolism; D004307:Dose-Response Relationship, Radiation; D015536:Down-Regulation; D006801:Humans; D007962:Leukocytes / Q000187:drug effects / Q000502:physiology* / Q000528:radiation effects; D053594:Myeloid Differentiation Factor 88 / Q000235:genetics; D012313:RNA / Q000096:biosynthesis; D051217:Toll-Like Receptor 9 / Q000235:genetics; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism; D014965:X-Rays
D004275:DNA, Ribosomal; C507779:MYD88 protein, human; D053594:Myeloid Differentiation Factor 88; C495371:TLR9 protein, human; D051217:Toll-Like Receptor 9; D014409:Tumor Necrosis Factor-alpha; D012313:RNA; D004247:DNA; D053148:Caspase 3