Effect of microphase separation on the protein resistance of a polymeric surface.
Segmented polyurethanes (PUs) containing poly(ethylene glycol) (PEG), poly(propylene glycol), or poly(dimethylsiloxane) soft segments have been prepared by two-step condensation polymerization. Atom force microscopy observation in air and solution indicates that the segmented PU forms a microphase separation on the surface. By use of quartz crystal microbalance with dissipation and surface plasmon resonance, we have investigated the adsorption of fibrinogen, bovine serum albumin, and lysozyme on a surface constructed by such a PU in aqueous solution in real time. Our results reveal that the protein resistance of the PUs arises from the hydrated PEG segments instead of microphase separation.
Hefei National Laboratory for Physical Sciences at Microscale, Department of Chemical Physics, University of Science and Technology of China, Hefei 230026, China.
Chunfeng
C
Ma
Yi
Y
Hou
Shi
S
Liu
Guangzhao
G
Zhang
D000042:Absorption; D000818:Animals; D002417:Cattle; D004129:Dimethylpolysiloxanes / Q000737:chemistry; D008832:Microchemistry; D018625:Microscopy, Atomic Force; D015394:Molecular Structure; D044367:Phase Transition; D011092:Polyethylene Glycols / Q000737:chemistry; D011108:Polymers / Q000737:chemistry; D011140:Polyurethanes / Q000737:chemistry*; D011409:Propylene Glycols / Q000737:chemistry; D011506:Proteins / Q000032:analysis / Q000737:chemistry*; D012710:Serum Albumin, Bovine; D013499:Surface Properties
D004129:Dimethylpolysiloxanes; D011108:Polymers; D011140:Polyurethanes; D011409:Propylene Glycols; D011506:Proteins; C012504:polypropylene glycol; D012710:Serum Albumin, Bovine; D011092:Polyethylene Glycols; C013830:baysilon