Annular pupil filter under shot-noise condition for linear and non linear microscopy.
The imaging performances of multiphoton excitation and confocal laser scanning microscopy are herby considered: in typical experimental imaging conditions, a small finite amount of photon reaches the detector giving shot-noise fluctuations which affects the signal acquired. A significant detriment in the high frequencies transmission capability is obtained. In order to partially recover the high frequencies information lost, the insertion of a pupil plane filter in the microscope illumination light pathway on the objective lens is proposed. We demonstrate high-frequency and resolution enhancement in the case of linear and non linear fluorescence microscope approach under shot-noise condition.
Ronzitti
Emiliano
E
LAMBS-IFOM, MicroScoBio, Department of Physics, University of Genoa, Via Dodecaneso, 16145 Genoa, Italy. ronzitti@fisica.unige.it
Vicidomini
Giuseppe
G
Caorsi
Valentina
V
Diaspro
Alberto
A
D003198:Computer Simulation; D017076:Computer-Aided Design; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005374:Filtration / Q000295:instrumentation* / Q000379:methods; D007089:Image Enhancement / Q000295:instrumentation*; D007909:Lenses; D016014:Linear Models; D018613:Microscopy, Confocal / Q000295:instrumentation* / Q000379:methods; D017711:Nonlinear Dynamics; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity