Optical microassembly platform for constructing reconfigurable microenvironments for biomedical studies.
Cellular development is highly influenced by the surrounding microenvironment. We propose user-reconfigurable microenvironments and bio-compatible scaffolds as an approach for understanding cellular development processes. We demonstrate a model platform for constructing versatile microenvironments by fabricating morphologically complex microstructures by two-photon polymerization (2PP) and then assembling these archetypal building blocks into various configurations using multiple, real-time configurable counterpropagating-beam (CB) traps. The demonstrated capacity for handling feature-rich microcomponents may be further developed into a generalized microassembly platform.
Rodrigo
Peter John
PJ
DTU Fotonik, Technical University of Denmark, DK-4000 Roskilde, Denmark.
Kelemen
Lóránd
L
Palima
Darwin
D
Alonzo
Carlo Amadeo
CA
Ormos
Pál
P
Glückstad
Jesper
J
D001698:Biomedical Engineering / Q000295:instrumentation*; D018929:Cell Culture Techniques / Q000295:instrumentation*; D002469:Cell Separation / Q000295:instrumentation*; D017076:Computer-Aided Design; D004867:Equipment Design; D019544:Equipment Failure Analysis; D055096:Optical Devices; D052898:Optical Tweezers; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity