In vitro kinetic study of growth and mineralization of osteoblast-like cells (Saos-2) on titanium surface coated with a RGD functionalized bisphosphonate.
Osteoconduction and osseointegration are the critical stages for implantation success. Peptides containing RGD (Arg-Gly-Asp) adhesive sequence are known to promote cell adhesion and consequently to favor osseointegration of medical devices. In this study, RGD peptides were coupled to a bisphosphonate used as an anchor system and chemically adsorbed on polished titanium discs. Two different concentrations, 10(-10) mol/L (RGD 10(-10)) and 10(-4) mol/L (RGD 10(-4)) were compared to non coated discs (RGD 0). Adhesion, spreading, and mineralization of osteoblast-like cells (Saos-2) were assessed. Mineralization kinetic was done at 3, 6, 10, 14, and 18 days of culture; the extent of mineral deposits was quantified by image analysis. Histogram repartitions of nuclear area, characterizing cell spreading, showed a shift to higher values in cells cultured on RGD coated titanium disks. Mineralization started at day 3 in the three groups, but had a faster development in the RGD 10(-10) group from day 6 to day 18 compared to RGD 0 and RGD 10(-4). At day 18, the percentage of mineralized area was significantly higher for RGD 10(-10) compared to RGD 0 (p < 0.05). In the present study, this new method was found suitable to anchor RGD containing species on titanium: this favored adhesion and spreading of osteoblast-like cells and mineralization compared to noncoated titanium.
INSERM, U922, LHEA, Faculté de Médecine, Angers, Cedex 49045, France.
Johanne
J
Beuvelot
David
D
Portet
Grégory
G
Lecollinet
Marie-Françoise
MF
Moreau
Michel Félix
MF
Baslé
Daniel
D
Chappard
Hélène
H
Libouban
D000327:Adsorption; D001672:Biocompatible Materials / Q000737:chemistry*; D002448:Cell Adhesion; D002460:Cell Line; D004164:Diphosphonates / Q000737:chemistry*; D006801:Humans; D007091:Image Processing, Computer-Assisted; D066298:In Vitro Techniques; D007700:Kinetics; D009842:Oligopeptides / Q000737:chemistry*; D010006:Osteoblasts / Q000166:cytology*; D010455:Peptides / Q000737:chemistry; D017550:Spectroscopy, Fourier Transform Infrared; D013499:Surface Properties; D013997:Time Factors; D014025:Titanium / Q000737:chemistry
D001672:Biocompatible Materials; D004164:Diphosphonates; D009842:Oligopeptides; D010455:Peptides; C047981:arginyl-glycyl-aspartic acid; D014025:Titanium