Photoconversion in orange and red fluorescent proteins.
We found that photoconversion is fairly common among orange and red fluorescent proteins, as in a screen of 12 proteins, 8 exhibited photoconversion. Specifically, three red fluorescent proteins could be switched to a green state, and two orange variants could be photoconverted to a far-red state. The orange proteins are ideal for dual-probe highlighter applications, and they exhibited the most red-shifted excitation of all fluorescent proteins described to date.
Department of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, Tennessee, USA.
Gert-Jan
GJ
Kremers
Kristin L
KL
Hazelwood
Christopher S
CS
Murphy
Michael W
MW
Davidson
David W
DW
Piston
D002470:Cell Survival / Q000528:radiation effects; D006367:HeLa Cells; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008164:Luminescent Proteins / Q000737:chemistry* / Q000235:genetics; D008856:Microscopy, Fluorescence; D055668:Photochemical Processes; D013050:Spectrometry, Fluorescence; D014162:Transfection; D000097573:Red Fluorescent Protein
D008164:Luminescent Proteins; D000097573:Red Fluorescent Protein; C513582:Kaede protein, Trachyphyllia geoffroyi; C413663:fluorescent protein 583