Electrophoretic mobility shift assays for the analysis of DNA-protein interactions.
Electromobility shift assay is a simple, efficient, and rapid method for the study of specific DNA-protein interactions. It relies on the reduction in the electrophoretic mobility conferred to a DNA fragment by an interacting protein. The technique is suitable to qualitative, quantitative, and kinetic analyses. It can also be used to analyze conformational changes.
Gaudreault
Manon
M
Oncology and Molecular Endocrinology Research Center, CHUL, Centre Hospitalier Universitaire de Québec and Laval University, 2705 Laurier Blvd, Québec, QC, Canada, G1V 4G2.
Gingras
Marie-Eve
ME
Lessard
Maryse
M
Leclerc
Steeve
S
Guérin
Sylvain L
SL
D004247:DNA / Q000378:metabolism*; D015342:DNA Probes / Q000302:isolation & purification; D024202:Electrophoretic Mobility Shift Assay / Q000379:methods*; D007553:Isotope Labeling; D009687:Nuclear Proteins / Q000378:metabolism; D011485:Protein Binding / Q000187:drug effects; D011506:Proteins / Q000378:metabolism*; D012965:Sodium Chloride / Q000494:pharmacology
D015342:DNA Probes; D009687:Nuclear Proteins; D011506:Proteins; D012965:Sodium Chloride; D004247:DNA