Analysis of bla(SHV) codon 238 and 240 allele mixtures using Sybr green high-resolution melting analysis.
Klebsiella pneumoniae isolates frequently contain complex mixtures of bla(SHV) alleles. A high-resolution melting-based method for interrogating the extended-spectrum activity conferring codon 238 and 240 polymorphisms was developed. This detects minority extended-spectrum beta-lactamase-encoding alleles, allows estimation of allele ratios, and discriminates between single and double mutants.
Andersson
Patiyan
P
Menzies School of Health Research, Institute of Advanced Studies, Charles Darwin University, Northern Territory 0810, Australia.
Harris
Tegan
T
Tong
Steven Y C
SY
Giffard
Philip M
PM
D000483:Alleles; D052160:Benzothiazoles; D003062:Codon; D003959:Diamines; D007711:Klebsiella pneumoniae / Q000235:genetics; D009930:Organic Chemicals; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide; D011804:Quinolines; D001618:beta-Lactamases / Q000235:genetics*
D052160:Benzothiazoles; D003062:Codon; D003959:Diamines; D009930:Organic Chemicals; D011804:Quinolines; C098022:SYBR Green I; C054882:beta-lactamase PIT-2; C107197:beta-lactamase SHV-11; C107198:beta-lactamase SHV-12; C062760:beta-lactamase SHV-2; C117193:beta-lactamase SHV-5; D001618:beta-Lactamases