Different embryonic development after blastomere biopsy for preimplantation genetic diagnosis, observed by time-lapse imaging.
Using time-lapse imaging, we found different behavior of mouse embryonal development after blastomere biopsy for preimplantation genetic diagnosis. Blastomere removal has effects on the developmental behavior of the mouse embryo, including speed of growth, contraction and expansion movements, and hatching.
Department of Obstetrics and Gynecology, Tohoku University School of Medicine, Sendai, Japan. Electronic address: terada@mail.tains.tohoku.ac.jp.
Yukihiro
Y
Terada
Department of Obstetrics and Gynecology, Tohoku University School of Medicine, Sendai, Japan.
Tomohisa
T
Ugajin
Department of Obstetrics and Gynecology, Tohoku University School of Medicine, Sendai, Japan.
Hisataka
H
Hasegawa
Department of Obstetrics and Gynecology, Tohoku University School of Medicine, Sendai, Japan.
Hiroshi
H
Nabeshima
Department of Obstetrics and Gynecology, Tohoku University School of Medicine, Sendai, Japan.
Nobuo
N
Yaegashi
D000818:Animals; D001706:Biopsy; D001757:Blastomeres / Q000473:pathology*; D002970:Cleavage Stage, Ovum / Q000473:pathology / Q000502:physiology; D003952:Diagnostic Imaging / Q000379:methods*; D004622:Embryo, Mammalian / Q000166:cytology / Q000473:pathology; D047108:Embryonic Development / Q000502:physiology*; D005260:Female; D006801:Humans; D051379:Mice; D018715:Microscopy, Video / Q000379:methods; D019836:Preimplantation Diagnosis / Q000379:methods*; D014741:Video Recording / Q000379:methods