Characterization of Powassan viruses from Far Eastern Russia.
We report the isolation and detailed characterization of the novel strain, Partizansk/2006, of Powassan virus (POWV) from a human case of infection, which occurred in Primorsky krai, Russia, in 2006. Comparative complete genome sequence analysis of the Far Eastern strains Spassk-9 (1975), Nadezdinsk-1991 and Partizansk/2006 of POWV revealed that these strains are 99.8% similar to the LB strain, which was isolated in Canada in 1958. Phylogenetic analysis of 5' UTR sequences of five other strains of POWV isolated from 1972 to 1986 in Primorsky krai produced similar results. Presumably, Far Eastern POWV has common putative ancestor with LB strain POWV from North America, and the time of divergence of these POWVs is relatively short. We conclude that POWV has become endemic in Far Eastern Russia.
Leonova
Galina N
GN
Institute of Epidemiology and Microbiology, Siberian Branch of the Russian Academy of Medical Sciences, Vladivostok, Russia.
Kondratov
Ilia G
IG
Ternovoi
Vladimir A
VA
Romanova
Elena V
EV
Protopopova
Elena V
EV
Chausov
Eugene V
EV
Pavlenko
Elena V
EV
Ryabchikova
Elena I
EI
Belikov
Sergey I
SI
Loktev
Valery B
VB
D000818:Animals; D001483:Base Sequence; D002478:Cells, Cultured; D055028:Comparative Genomic Hybridization; D004669:Encephalitis Viruses, Tick-Borne / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D004675:Encephalitis, Tick-Borne / Q000821:virology; D019143:Evolution, Molecular; D016679:Genome, Viral; D006801:Humans; D046529:Microscopy, Electron, Transmission; D008969:Molecular Sequence Data; D009500:Neutralization Tests; D009690:Nucleic Acid Conformation; D010802:Phylogeny; D012367:RNA, Viral / Q000235:genetics; D012426:Russia; D017423:Sequence Analysis, RNA; D013552:Swine
D012367:RNA, Viral