Cognitive enhancement by SGS742 in OF1 mice is linked to specific hippocampal protein expression.
Cognitive enhancement by the GABA (B) antagonist SGS742 has been reported and we decided to search for proteins involved. Hippocampi from OF1 mice were from SGS742- treated animals and three control groups. Proteins were extracted and run on 2DE, and spots were quantified. Significantly different protein spots were identified by two mass spectrometry principles, nano-LC-ESI-MS/MS and by nano-LC-ESI(CID/ETD)-MS/MS. Signaling, chaperone and metabolic enzyme proteins were linked to memory enhancement.
Sunyer
Berta
B
Department of Pediatrics, Medical University of Vienna, Vienna, Austria.
Diao
Wei-Fei
WF
Kang
Sung Ung
SU
An
Gunyong
G
Boddul
Sanjay
S
Lubec
Gert
G
D000818:Animals; D003071:Cognition; D015180:Electrophoresis, Gel, Two-Dimensional; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D006624:Hippocampus / Q000378:metabolism*; D051379:Mice; D018832:Molecular Chaperones / Q000378:metabolism; D018696:Neuroprotective Agents / Q000494:pharmacology; D009943:Organophosphorus Compounds / Q000494:pharmacology; D011506:Proteins / Q000737:chemistry*; D040901:Proteomics / Q000379:methods*; D015398:Signal Transduction; D021241:Spectrometry, Mass, Electrospray Ionization; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D018832:Molecular Chaperones; D018696:Neuroprotective Agents; D009943:Organophosphorus Compounds; D011506:Proteins; C079148:(3-aminopropyl)(n-butyl)phosphinic acid