Rapid and sensitive LC-MS method for pharmacokinetic study of vinorelbine in rats.
A rapid and sensitive LC-electrospray ionization-MS method was developed for determining vinorelbine in rat plasma. A 100 microL plasma sample was treated using a protein precipitation procedure and was chromatographed within 4 min using an Inertsil ODS-3 C(18 )(2.1 x 50 mm, 5 microm) column. The selected ion monitoring ions [M + H](+) were m/z 779 and m/z 811 for vinorelbine and vinblastine (internal standard), respectively. The method validation showed that the calibration curve for vinorelbine was linear over a concentration range of 1-1000 ng/mL with lower limit of quantification at 1 ng/mL. The method has been successfully applied to pharmacokinetics in rat plasma.
Key Laboratory for Special Functional Materials, Henan University, Kaifeng, People's Republic of China.
Tiefeng
T
Cheng
Duanyun
D
Si
Changxiao
C
Liu
D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000097:blood*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D051381:Rats; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D014747:Vinblastine / Q000031:analogs & derivatives* / Q000097:blood; D000077235:Vinorelbine
D000972:Antineoplastic Agents, Phytogenic; D014747:Vinblastine; D000077235:Vinorelbine