Parallel downregulation of retinol-binding protein-4 and adiponectin expression in subcutaneous adipose tissue of non-morbidly obese subjects.
OBJECTIVE
Adipokines are involved in the etiopathology of obesity-related disorders. Since the role of adipokine retinol-binding protein-4 (RBP4) in obesity remains uncertain and its relationship with other adipokines and inflammatory markers has not been examined in detail, we investigated the relationships of RBP4 mRNA expression and circulating protein levels with obesity, anthropometric and metabolic variables, as well as with obesity-related inflammatory markers adiponectin and C-reactive protein.


METHODS
One-hundred and twenty-five subjects participated, 36 lean (body mass index (BMI): <25 kg/m(2)) and 89 obese (overweight/obese; BMI: > or =25<40) whose anthropometric and metabolic variables were assessed. mRNA expression was quantified by real-time PCR in subcutaneous adipose tissue (s.c.-AT) of 46 subjects.


RESULTS
There was a tendency for circulating RBP4 levels to positively correlate with waist circumference (beta=0.29, P=0.08; R(2)=0.08), but there was no significant association with the obesity-related parameters analysed. RBP4 and adiponectin mRNA expression levels were similarly downregulated in the s.c.-AT of obese subjects (0.5-fold); however, RBP4 downregulation did not affect its circulating protein levels. The expression of RBP4 and adiponectin was positively correlated even after controlling for confounding factors (beta=0.59, P<0.0001; R(2)=0.40).


CONCLUSIONS
In our population, RBP4 circulating levels were not significantly correlated with obesity-related parameters, although a tendency to correlate with waist circumference suggests a relationship with insulin resistance and other metabolic disorders. In addition, our results suggest that the production of RBP4 by other tissues such as liver, rather than s.c.-AT, may be involved in regulating RBP4 circulating levels.
CIBER (CB06/03) Fisiopatologia de la Obesidad y Nutrición, Instituto de Salud Carlos III, Avda Monforte de Lemos, Madrid, Spain. mbroch.hj23.ics@gencat.cat
Montserrat
M
Broch
Maria Teresa
MT
Auguet
Rafael
R
Ramírez
Montserrat
M
Olona
Carmen
C
Aguilar
Ana
A
Megia
Maria José
MJ
Alcaide
Rosa
R
Pastor
Salomé
S
Martínez
Enric
E
Caubet
Antonio
A
Garcia-España
Cristóbal
C
Richart
D017667:Adipocytes / Q000502:physiology; D052242:Adiponectin / Q000097:blood / Q000235:genetics; D000328:Adult; D000368:Aged; D001827:Body Height; D001835:Body Weight; D002097:C-Reactive Protein / Q000378:metabolism; D015536:Down-Regulation / Q000502:physiology; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009765:Obesity / Q000378:metabolism / Q000503:physiopathology*; D012333:RNA, Messenger / Q000378:metabolism; D054839:Retinol-Binding Proteins, Plasma / Q000235:genetics* / Q000378:metabolism; D012720:Severity of Illness Index; D017154:Stromal Cells / Q000502:physiology; D050151:Subcutaneous Fat / Q000166:cytology / Q000502:physiology*
C499918:ADIPOQ protein, human; D052242:Adiponectin; C101326:RBP4 protein, human; D012333:RNA, Messenger; D054839:Retinol-Binding Proteins, Plasma; D002097:C-Reactive Protein