Characterization of P450 FcpC, the enzyme responsible for bioconversion of diosgenone to isonuatigenone in Streptomyces virginiae IBL-14.
A new cytochrome P450 monooxygenase, FcpC, from Streptomyces virginiae IBL-14 has been identified. This enzyme is found to be responsible for the bioconversion of a pyrano-spiro steroid (diosgenone) to a rare nuatigenin-type spiro steroid (isonuatigenone), which is a novel C-25-hydroxylated diosgenone derivative. A whole-cell P450 system was developed for the production of isonuatigenone via the expression of the complete three-component electron transfer chain in an Escherichia coli strain.
Wang
Wei
W
New World Institute of Biotechnology, East China University of Science and Technology, Shanghai, China.
Wang
Feng-Qing
FQ
Wei
Dong-Zhi
DZ
D001711:Biotransformation; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics; D015870:Gene Expression; D023061:Gene Order; D008969:Molecular Sequence Data; D009251:NADPH-Ferrihemoprotein Reductase / Q000235:genetics / Q000378:metabolism*; D017422:Sequence Analysis, DNA; D013141:Spiro Compounds / Q000378:metabolism*; D013302:Streptomyces / Q000201:enzymology* / Q000235:genetics; D014315:Triterpenes / Q000378:metabolism*
D013141:Spiro Compounds; D014315:Triterpenes; C541553:isonuatigenone; C469690:diosgenone; D009251:NADPH-Ferrihemoprotein Reductase