A mitogen-activated protein kinase gene, AhMPK3 of peanut: molecular cloning, genomic organization, and heterologous expression conferring resistance against Spodoptera litura in tobacco.
Mitogen-activated protein kinase cascade plays a very important role in plant signal transduction mechanism. A full length cDNA of 1,514 bp length, corresponding to a mitogen-activated protein kinase gene was cloned from peanut (Arachis hypogaea). Based on its high homology with Arabidopsis AtMPK3, the cDNA was designated as AhMPK3. It carried an open reading frame of 1,113 bp encoding a 371 amino acid polypeptide. AhMPK3 bears TEY motif in its activation loop and belongs to the A1 subgroup of MAPK family. Southern blot analysis revealed that AhMPK3 exists in two copies in peanut genome and its structural organization revealed well-conserved nature of these signaling components across different species. AhMPK3 when transiently expressed in tobacco leaves was found to localize in both nucleus and cytoplasm. Transgenic tobacco plants ectopically expressing AhMPK3 exhibited enhanced resistance to first and second instar larvae of Spodoptera litura and constitutively higher transcript levels of defense response genes like PR1a, PR1b, LOX1, PI-II etc. Apart from this when wounded, transgenic plants accumulated high levels of PI-II and PR1b transcripts rapidly compared to wild type indicating the occurrence of a priming phenomenon.
Department of Plant Sciences, University of Hyderabad, Hyderabad 500046, India.
Koppolu Raja Rajesh
KR
Kumar
Tantravahi
T
Srinivasan
Pulugurtha Bharadwaja
PB
Kirti
D000595:Amino Acid Sequence; D000818:Animals; D010367:Arachis / Q000201:enzymology* / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D017931:DNA Primers / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics; D018744:DNA, Plant / Q000235:genetics; D018628:Gene Dosage; D015870:Gene Expression; D017343:Genes, Plant; D018745:Genome, Plant; D020928:Mitogen-Activated Protein Kinases / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D010802:Phylogeny; D010935:Plant Diseases / Q000235:genetics / Q000469:parasitology; D030821:Plants, Genetically Modified; D017386:Sequence Homology, Amino Acid; D018411:Spodoptera / Q000472:pathogenicity; D014026:Nicotiana / Q000201:enzymology / Q000235:genetics / Q000469:parasitology
D017931:DNA Primers; D018076:DNA, Complementary; D018744:DNA, Plant; D020928:Mitogen-Activated Protein Kinases