Gastrin releasing protein receptor specific gold nanorods: breast and prostate tumor avid nanovectors for molecular imaging.
Gastrin releasing protein receptor specific bombesin (BBN) peptide-gold nanoconjugates were successfully synthesized using gold nanorods and dithiolated peptide. The gold nanorod-bombesin (GNR-BBN) conjugates showed extraordinary in vitro stabilities against various biomolecules including NaCl, cysteine, histidine, bovine serum albumin, human serum albumin, and dithiothreitol. Quantitative measurements on the binding affinity (IC(50)) of GNR-BBN conjugates toward prostate and breast tumor cells were evaluated. The IC(50) values establish that GNR-BBN conjugates have strong affinity toward the gastrin releasing peptide receptors on both the tumors. Detailed cellular interaction studies of GNR-BBN conjugates revealed that nanorods internalize via a receptor-mediated endocytosis pathway. The receptor specific interactions of GNR-BBN conjugates provide realistic opportunities in the design and development of in vivo molecular imaging and therapy agents for cancer.
Department of Radiology, University of Missouri-Columbia, Columbia, Missouri 65212, USA.
Nripen
N
Chanda
Ravi
R
Shukla
Kattesh V
KV
Katti
Raghuraman
R
Kannan
D000818:Animals; D001839:Bombesin / Q000737:chemistry; D001943:Breast Neoplasms / Q000737:chemistry*; D002417:Cattle; D045744:Cell Line, Tumor; D004705:Endocytosis; D005260:Female; D006046:Gold / Q000737:chemistry*; D006801:Humans; D008297:Male; D053768:Metal Nanoparticles / Q000737:chemistry*; D051379:Mice; D046529:Microscopy, Electron, Transmission; D041681:NIH 3T3 Cells; D043942:Nanotubes / Q000737:chemistry*; D011471:Prostatic Neoplasms / Q000737:chemistry*; D018004:Receptors, Bombesin / Q000737:chemistry*
D018004:Receptors, Bombesin; D006046:Gold; D001839:Bombesin