[Visualization of viruses in living cells].
Live-cell imaging of cells infected with recombinant viruses expressing fluorescently tagged structural viral proteins enables to visualize virion maturation pathways at a virion particle level in the same cells as infection progresses. This technology have gradually unveiled previously unreported aspects of the pathways. This article focuses on live-cell imaging technology of herpes simplex virus 1 (HSV-1) and reviews the up-to-date topics of HSV-1 maturation pathway including our recent progress.
Division of Viral Infection, Department of Infectious Disease Control, International Research Center for Infectious Diseases, Institute of Medical Science, The University of Tokyo, Tokyo, Japan. ykawagu@ims.u-tokyo.ac.jp
Yasushi
Y
Kawaguchi
D000818:Animals; D002522:Chlorocebus aethiops; D005456:Fluorescent Dyes; D018259:Herpesvirus 1, Human / Q000502:physiology* / Q000648:ultrastructure*; D006801:Humans; D018613:Microscopy, Confocal; D014709:Vero Cells / Q000821:virology*; D014771:Virion / Q000502:physiology / Q000648:ultrastructure; D014779:Virus Replication
D005456:Fluorescent Dyes