Differential thiol status in blood of different mouse strains exposed to cigarette smoke.
C57Bl/6J, DBA/2 and ICR mouse strains are known to possess different susceptibilities to developing emphysema after exposure to cigarette smoke with DBA/2 and C57Bl/6J strains being significantly more susceptible to pulmonary damage than the ICR strain. This study was aimed at analysing the occurrence of systemic oxidative stress in the blood of these different mouse strains after exposure to cigarette smoke. This study did not observe a significant decrease in glutathione in erythrocytes or in plasma cysteine, cysteinylglycine, homocysteine and glutathione in C57Bl/6J or DBA/2 mice, whereas a significant increase in the corresponding oxidized forms was observed in plasma. However, the ICR strain showed a significant increase in glutathione in erythrocytes and a significant decrease in most of the oxidized forms of cysteine, cysteinylglycine, homocysteine and glutathione in plasma after the same exposition. These experiments demonstrate that exposure to cigarette smoking induces systemic oxidative stress only in some mouse strains which are susceptible to developing emphysema.
Department of Evolutionary Biology, Laboratory of Pharmacology and Toxicology, Experimental Medicine & Public Health, University of Siena, Siena, Italy.
Ranieri
R
Rossi
Daniela
D
Giustarini
Silvia
S
Fineschi
Giovanna
G
De Cunto
Giuseppe
G
Lungarella
Eleonora
E
Cavarra
D000818:Animals; D003545:Cysteine / Q000097:blood; D004151:Dipeptides / Q000097:blood; D004646:Emphysema / Q000235:genetics; D020022:Genetic Predisposition to Disease; D005978:Glutathione / Q000097:blood; D019803:Glutathione Disulfide / Q000097:blood; D006710:Homocysteine / Q000097:blood; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D008813:Mice, Inbred ICR; D018384:Oxidative Stress / Q000502:physiology*; D013438:Sulfhydryl Compounds / Q000097:blood*; D014028:Tobacco Smoke Pollution / Q000009:adverse effects*
D004151:Dipeptides; D013438:Sulfhydryl Compounds; D014028:Tobacco Smoke Pollution; D006710:Homocysteine; C028505:cysteinylglycine; D005978:Glutathione; D003545:Cysteine; D019803:Glutathione Disulfide