Cross-presentation of viral and self antigens by skin-derived CD103+ dendritic cells.
Skin-derived dendritic cells (DCs) include Langerhans cells, classical dermal DCs and a langerin-positive CD103(+) dermal subset. We examined their involvement in the presentation of skin-associated viral and self antigens. Only the CD103(+) subset efficiently presented antigens of herpes simplex virus type 1 to naive CD8(+) T cells, although all subsets presented these antigens to CD4(+) T cells. This showed that CD103(+) DCs were the migratory subset most efficient at processing viral antigens into the major histocompatibility complex class I pathway, potentially through cross-presentation. This was supported by data showing only CD103(+) DCs efficiently cross-presented skin-derived self antigens. This indicates CD103(+) DCs are the main migratory subtype able to cross-present viral and self antigens, which identifies another level of specialization for skin DCs.
The Department of Microbiology and Immunology, The University of Melbourne, Parkville, Victoria, Australia.
Sammy
S
Bedoui
Paul G
PG
Whitney
Jason
J
Waithman
Liv
L
Eidsmo
Linda
L
Wakim
Irina
I
Caminschi
Rhys S
RS
Allan
Magdalena
M
Wojtasiak
Ken
K
Shortman
Francis R
FR
Carbone
Andrew G
AG
Brooks
William R
WR
Heath
D000818:Animals; D017951:Antigen Presentation / Q000276:immunology; D015703:Antigens, CD / Q000276:immunology; D000956:Antigens, Viral / Q000276:immunology*; D001324:Autoantigens / Q000276:immunology*; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D002465:Cell Movement / Q000276:immunology; D045142:Cross-Priming / Q000276:immunology*; D003713:Dendritic Cells / Q000276:immunology*; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D006561:Herpes Simplex / Q000276:immunology; D018259:Herpesvirus 1, Human / Q000276:immunology; D015395:Histocompatibility Antigens Class I / Q000276:immunology; D039001:Integrin alpha Chains / Q000276:immunology; D008213:Lymphocyte Activation / Q000276:immunology*; D051379:Mice; D008822:Mice, Transgenic; D012867:Skin / Q000166:cytology / Q000276:immunology* / Q000821:virology
D015703:Antigens, CD; D000956:Antigens, Viral; D001324:Autoantigens; D015395:Histocompatibility Antigens Class I; D039001:Integrin alpha Chains; C091367:alpha E integrins