The variation in cytoplasmic distribution of mouse peritoneal macrophage during phagocytosis modulated by mangiferin, an immunomodulator.
The peritoneal MPhi in the immunological defense removes foreign particles, pathogenic or otherwise, by phagocytosis, and shows movement in search of the target. The macrophage (MPhi) appears in various shapes and sizes, spherical, flattened spindle-shaped, amoeboid, polygonal and with very long extension. On activation, the MPhi shows changes in cell shape, cytokinesis and development of intercellular contacts. A dynamic redistribution of cytoskeleton with cytoplasmic spread and/or extensions occurs with immunomodulators, like Mangiferin (1,3,6,7-tetrahydroxyxanthone-C(2)-beta-D-glucoside). The MPhi isolated from the peritoneal fluid of BALB/c mice pretreated with mangiferin and saline control, on challenge, shows redistribution of cytoplasm with variable morphology. Using the image analyses technique for pattern recognition of individual shapes of MPhi leads to the observation of clustering of cells in the coverslip culture. The stimulation in phagocytosis due to mangiferin, shows cytoplasmic spread, long extensions and intercellular contacts. The individual variations in the cytoplasmic redistribution are due to changes in the balance between the cellular surface area and the long extensions where the shape and nature of the phagocytic particle and the type of MPhi are determinants.
Department of Life Science and Biotechnology, Jadavpur University, Kolkata 700032, India.
Atasi
A
De
Sukumar
S
Chattopadhyay
D000818:Animals; D002286:Carcinoma, Ehrlich Tumor / Q000276:immunology; D002457:Cell Extracts; D022081:Cell Surface Extensions / Q000187:drug effects / Q000276:immunology / Q000648:ultrastructure; D002478:Cells, Cultured; D002645:Chickens; D029802:Croton / Q000276:immunology; D003593:Cytoplasm / Q000276:immunology* / Q000378:metabolism / Q000648:ultrastructure*; D004912:Erythrocytes / Q000276:immunology / Q000378:metabolism; D006801:Humans; D007155:Immunologic Factors / Q000494:pharmacology*; D007840:Latex / Q000276:immunology; D008262:Macrophage Activation / Q000187:drug effects / Q000276:immunology; D017737:Macrophages, Peritoneal / Q000187:drug effects / Q000276:immunology* / Q000648:ultrastructure*; D051379:Mice; D008807:Mice, Inbred BALB C; D010587:Phagocytosis / Q000187:drug effects* / Q000276:immunology; D021381:Protein Transport / Q000187:drug effects / Q000276:immunology; D044004:Xanthones / Q000494:pharmacology*; D015054:Zymosan / Q000276:immunology
D002457:Cell Extracts; D007155:Immunologic Factors; D007840:Latex; D044004:Xanthones; C013592:mangiferin; D015054:Zymosan