Changes in Borna disease virus genome with adaptation to host.
The CRNP5 variant of Borna disease virus (BDV) has stronger pathogenesis than the CRP3 variant in which only 4 nucleotides in the whole genome are different. The CRP3 is produced by 3 passages in rat brains of BDV, whereas the CRNP5 is produced by 5 passages in mouse brains after 2 passages in rat brains of the BDV. Thymidylic acids at nt 3608 and 3673 were replaced by cytidylic acids during 3 passages in mice. Three passages in mice caused replacement of adenylic acid at nt 7936 by guanylic acid. No replacement at nt 8742 occurred during passages in mice.
Okayama
Satoshi
S
Laboratory of Veterinary Immunology, Azabu University School of Veterinary Medicine, Fuchinobe, Sagamihara, Japan.
Miura
Nanako
N
Murakami
Masaru
M
Funaba
Masayuki
M
Nishino
Yoshii
Y
D000220:Adaptation, Biological; D000818:Animals; D000831:Animals, Newborn; D001891:Borna disease virus / Q000235:genetics* / Q000254:growth & development*; D001921:Brain / Q000821:virology; D016679:Genome, Viral; D051379:Mice; D017354:Point Mutation; D051381:Rats; D012692:Serial Passage