[The application and significance in prenatal diagnosis using G-banding, fluorescence in situ hybridization and comparative genomic hybridization].
OBJECTIVE
To investigate the procedure and the value of G-banding, fluorescence in sit hybridization (FISH) and comparative genomic hybridization (CGH) techniques in prenatal diagnosis.


METHODS
Karyotype analyses with three diagnostic procedures, G-banding, G-banding and FISH, G-banding, FISH and CGH, were performed in the amniotic fluid samples taken from 102 fetuses at gestational ages 16-24 weeks. And the significance was valued in prenatal diagnosis.


RESULTS
In the first procedure of karyotype analysis, 98 cases were diagnosed, 2 cases were not conformed while 2 cases were failed in all 102 cases. In the second procedure, 2 cases were determined, 1 case was not conformed and 1 case was still failed. In the third step, 2 cases were diagnosed. The diagnostic rate of the karyotype reached to 100% (102/102 cases) using all the three procedures. In total, seven cases with chromosomal abnormality were diagnosed. Four cases, 1 case and 2 cases were identified in the first step (4/7, 57.1%), the second (1/7, 14.3%) and the third (2/7, 28.5%), respectively.


CONCLUSIONS
It can help improve the diagnostic rate of chromosomal aberrations and standardize diagnostic procedure to perform the three detecting steps in prenatal diagnosis.
Central South University, Changsha, Hunan, 410008 People's Republic of China.
Wei-she
WS
Zhang
Qi-neng
QN
Chen
Xin-hua
XH
Wu
Qing-hua
QH
Liang
D002869:Chromosome Aberrations / Q000706:statistics & numerical data*; D002871:Chromosome Banding / Q000379:methods; D025063:Chromosome Disorders / Q000175:diagnosis* / Q000235:genetics; D002887:Chromosomes, Human, Pair 18; D055028:Comparative Genomic Hybridization / Q000379:methods; D005260:Female; D005333:Fetus; D005865:Gestational Age; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008607:Intellectual Disability / Q000235:genetics*; D007621:Karyotyping / Q000379:methods; D008297:Male; D009693:Nucleic Acid Hybridization / Q000379:methods; D011247:Pregnancy; D011296:Prenatal Diagnosis / Q000706:statistics & numerical data*; D012307:Risk Factors; D016216:Ultrasonography, Prenatal / Q000379:methods