High peroxidase activity and stable changes in the cell wall are related to dichlobenil tolerance.
Suspension-cultured bean cells habituated to growth in a lethal concentration of dichlobenil were cultured for 3-5 years in a medium lacking the inhibitor in order to obtain long-term dehabituated cell lines. The growth parameters, cell morphology and ultrastructure of cells in the absence of dichlobenil reverted to that of non-habituated cells. The cellulose content and Fourier transform infrared (FTIR) spectra of crude cell walls from long-term dehabituated cells were also similar to those of non-habituated cells. However, long-term dehabituated cells showed three times more tolerance to dichlobenil than non-habituated cells. The incorporation of [(14)C]Glc into cellulose was reduced by 40% in dehabituated cells when compared with non-habituated cells. However, the addition of dichlobenil to dehabituated cells increased the incorporation of [(14)C]Glc into cellulose 3.3-fold with respect to that of non-habituated cells. Dehabituated cells showed a constitutively increased peroxidase activity when compared with non-habituated cells. Results reported here indicate that the habituation of bean cultured cells to dichlobenil relied partially on a stable change in the cellulose biosynthesis complex and is associated with high guaiacol peroxidase activity.
García-Angulo
Penélope
P
Área de Fisiología Vegetal, Facultad de CC. Biológicas y Ambientales, Universidad de León, E-24071 León, Spain.
Alonso-Simón
Ana
A
Área de Fisiología Vegetal, Facultad de CC. Biológicas y Ambientales, Universidad de León, E-24071 León, Spain.
Mélida
Hugo
H
Área de Fisiología Vegetal, Facultad de CC. Biológicas y Ambientales, Universidad de León, E-24071 León, Spain.
Encina
Antonio
A
Área de Fisiología Vegetal, Facultad de CC. Biológicas y Ambientales, Universidad de León, E-24071 León, Spain.
Acebes
José L
JL
Área de Fisiología Vegetal, Facultad de CC. Biológicas y Ambientales, Universidad de León, E-24071 León, Spain. Electronic address: jl.acebes@unileon.es.
Álvarez
Jesús M
JM
Área de Fisiología Vegetal, Facultad de CC. Biológicas y Ambientales, Universidad de León, E-24071 León, Spain.
D000222:Adaptation, Physiological / Q000187:drug effects*; D000975:Antioxidants / Q000378:metabolism; D060387:Ascorbate Peroxidases; D018533:Biomass; D002449:Cell Aggregation / Q000187:drug effects; D002473:Cell Wall / Q000187:drug effects* / Q000201:enzymology* / Q000648:ultrastructure; D002478:Cells, Cultured; D002482:Cellulose / Q000378:metabolism; D007887:Fabaceae / Q000187:drug effects / Q000201:enzymology / Q000254:growth & development / Q000648:ultrastructure; D005947:Glucose / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000378:metabolism; D009570:Nitriles / Q000494:pharmacology*; D010544:Peroxidases / Q000378:metabolism*; D017550:Spectroscopy, Fourier Transform Infrared
D000975:Antioxidants; D009570:Nitriles; D002482:Cellulose; D010544:Peroxidases; D060387:Ascorbate Peroxidases; D005979:Glutathione Peroxidase; D005947:Glucose; C055869:dichlobanil