Study of the local immune response to Fasciola hepatica in the liver and hepatic lymph nodes of goats immunised with a peptide of the Sm14 antigen.
The nature of the local immune response was assessed studying the distribution of CD2(+), CD4(+), CD8(+), gammadelta(+) T lymphocytes, IgM(+) B cells, IL-4(+) and IFN-gamma(+) cells in the liver and hepatic lymph nodes (HLN) of goats immunised with a synthetic peptide of the Sm14 antigen from Schistosoma mansoni and challenged with Fasciola hepatica. A morphometric study of HLN was also carried out in order to evaluate the hyperplasia of lymphoid follicles. Despite the decrease in fluke burdens found in the immunised group (45.9%) respect to the infected control group, this difference was not statistically significant due to the high individual variability. In liver, a significant increase of CD2(+), CD4(+), CD8(+), gammadelta(+) T lymphocytes was found in the infected groups respect to the uninfected control and in the infected control respect to the immunised group. HLN showed a significant enlargement due to the hyperplasia of lymphoid follicles and infiltration of CD2(+), CD4(+), CD8(+), gammadelta(+) T lymphocytes in both infected groups respect to the uninfected control, with no significant differences between the infected control and immunised group. IFN-gamma(+) lymphoid cells was absent or very occasional in HLN where the number of IL-4(+) cells was higher than that of IFN-gamma, suggesting a polarized Th2 response in immunised and in infected control group.
Zafra
R
R
Department of Anatomy and Comparative Pathology, Faculty of Veterinary Medicine, University of Córdoba, 14014 Cordoba, Spain.
Buffoni
L
L
Pérez-Ecija
R A
RA
Mendes
R E
RE
Martínez-Moreno
A
A
Martínez-Moreno
F J
FJ
Pérez
J
J
D000818:Animals; D000947:Antigens, Helminth / Q000032:analysis*; D001402:B-Lymphocytes / Q000276:immunology / Q000469:parasitology; D005210:Fasciola hepatica / Q000276:immunology / Q000302:isolation & purification*; D015511:Goat Diseases / Q000469:parasitology*; D006041:Goats; D007075:Immunoglobulin M / Q000032:analysis; D007371:Interferon-gamma / Q000032:analysis; D015847:Interleukin-4 / Q000032:analysis; D008099:Liver / Q000276:immunology / Q000469:parasitology*; D008198:Lymph Nodes / Q000276:immunology / Q000469:parasitology*; D013601:T-Lymphocytes / Q000276:immunology / Q000469:parasitology
D000947:Antigens, Helminth; D007075:Immunoglobulin M; D015847:Interleukin-4; D007371:Interferon-gamma