Polysaccharide-based polyelectrolyte complex nanoparticles from chitosan, heparin, and hyaluronan.
The formation of polyelectrolyte complex nanoparticles (PCN) was investigated at different charge mixing ratios for the chitosan-heparin (chi-hep) and chitosan-hyaluronan (chi-ha) polycation-polyanion pairs. The range of 0.08-19.2 for charge mixing ratio (n(+)/n(-)) was examined. The one-shot addition of polycation and polyanion solutions used for the formation of the PCN permitted formation of both cationic and anionic particles from both polysaccharide pairs. The influence of the charge mixing ratio on the size and zeta potential of the particles was investigated. The morphology and stability of the particles when adsorbed to surfaces was studied by scanning electron microscopy (SEM). For most conditions studied, colloidally stable, nonstoichiometric PCN were formed in solution. However, PCN formation was inhibited by flocculation at charge mixing ratios near 1. When adsorbed to surfaces and dried, some formulations resulted in discrete nanoparticles, while others partially or completely aggregated or coalesced, leading to different surface morphologies.
Department of Chemical and Biological Engineering and School of Biomedical Engineering, Colorado State University, Fort Collins, Colorado 80523, USA.
Soheil
S
Boddohi
Nicholas
N
Moore
Patrick A
PA
Johnson
Matt J
MJ
Kipper
D002240:Carbohydrate Sequence; D048271:Chitosan / Q000737:chemistry*; D004573:Electrolytes / Q000737:chemistry*; D006493:Heparin / Q000737:chemistry*; D006820:Hyaluronic Acid / Q000737:chemistry*; D008855:Microscopy, Electron, Scanning; D053758:Nanoparticles; D011134:Polysaccharides / Q000737:chemistry*
D004573:Electrolytes; D011134:Polysaccharides; D006820:Hyaluronic Acid; D006493:Heparin; D048271:Chitosan