Methicillin-resistant Staphylococcus aureus fomite survival.
OBJECTIVE
To assess survival of methicillin-resistant Staphylococcus aureus (MRSA) on fomites encountered by health students.


METHODS
Three suspensions of MRSA were made to mimic lab splashes: a 0.5 McFarland trypticase soy broth, whole blood with 50 colony forming units/mL and body fluid/serum with 2000 colony forming units/mL. These were seeded onto three environmental surfaces (glass, vinyl floor tile, and countertop) and wet swabbed for 60 days. High touch areas of student stethoscopes were also wet swabbed. MRSA selective CHROMagar was used to identify organism survival.


METHODS
Salisbury University, Salisbury MD PARTICIPANTS: Salisbury University nursing and respiratory therapy students who volunteered to have their stethoscopes swabbed anonymously.


METHODS
Detection of pink colonies on MRSA-selective CHROMagar.


RESULTS
MRSA in 0.5 McFarland broth lived for > or =60 days on all surfaces. MRSA in blood was undetectable on any surface, and MRSA in serum survived 41 days on glass, 45 days on tile, and > or =60 days on countertop. Five of thirty-three stethoscopes (15%) tested were positive for MRSA.


CONCLUSIONS
Previous studies showed fomite survival of MRSA for about two weeks using contact plate sampling and MRSA on 7.4% of stethoscopes. We showed longer MRSA survival times by wet swab sampling and a higher stethoscope contamination rate. As expected, higher organism loads survived longer.
Clinical Laboratory Science Program, Health Sciences Department, Salisbury University, Salisbury, MD 21801, USA.
Christa
C
Williams
Diane L
DL
Davis
D003428:Cross Infection / Q000517:prevention & control; D050456:Fomites / Q000382:microbiology*; D006801:Humans; D055624:Methicillin-Resistant Staphylococcus aureus / Q000254:growth & development* / Q000302:isolation & purification; D013203:Staphylococcal Infections / Q000517:prevention & control; D013211:Staphylococcus aureus / Q000254:growth & development / Q000302:isolation & purification; D019023:Stethoscopes / Q000382:microbiology