Characterization of sheep (Ovis aries) palatine tonsil innervation.
Palatine tonsils (PTs), together with ileal Peyer's patches, rank among the first colonization sites for infectious prions. After replicating in these lymphoid tissues, prions undertake the process of "neuroinvasion," which is likely mediated by the peripheral nerves connecting lymphoid tissues to the central nervous system (CNS). To study the connections between the tonsils and the CNS, we injected fluorescent tracers into the PTs of lambs; the highest number of Fast Blue (FB)-labeled neurons was found in cranial cervical ganglia (CCG), whereas a progressively decreasing number of cells were detected in proximal glossopharyngeal, proximal vagal, trigeminal, pterygopalatine, and cervicothoracic ganglia. Immunohistochemistry was carried out on tonsil and ganglia cryosections. Immunoreactivity (IR) for tyrosine hydroxylase (TH), dopamine beta-hydroxylase (DBH), neuronal nitric oxide synthase (nNOS), calcitonin gene-related peptide (CGRP), substance P (SP), and calcium-binding protein S100 (S100), was observed in the fibers around and within PT lymphoid nodules. In the trigeminal, proximal glossopharyngeal and vagal ganglia the retrogradely-labeled neurons showed nNOS-, SP- and CGRP-IR. In all ganglia some retrogradely-labeled neurons showed nNOS-, SP- and CGRP-IR co-localization. It is worth noting that only 66+/-19% and 75+/-13% of retrogradely-labeled neurons in CCG showed TH- and DBH-IR, respectively. The present results allow us to attribute PT innervation mainly to the sympathetic component and to the glossopharyngeal, vagal and trigeminal cranial nerves. Furthermore, these data also provide a plausible anatomic route through which infectious agents, such as prions, may access the CNS, i.e. by traveling along several cranial and sympathetic nerves, as well as by migration via glial cells.
Russo
D
D
Department of Veterinary Morphophysiology and Animal Productions, Faculty of Veterinary Medicine, University of Bologna, Ozzano dell'Emilia (BO), Italy. domrusso@unina.it
Fantaguzzi
C Mongardi
CM
Di Guardo
G
G
Clavenzani
P
P
Costerbosa
G Lalatta
GL
Ligios
C
C
Chiocchetti
R
R
D000818:Animals; D001341:Autonomic Nervous System / Q000033:anatomy & histology / Q000378:metabolism; D015740:Calcitonin Gene-Related Peptide / Q000378:metabolism; D048429:Cell Size; D004299:Dopamine beta-Hydroxylase / Q000378:metabolism; D005260:Female; D017950:Ganglia, Sensory / Q000033:anatomy & histology* / Q000378:metabolism; D005728:Ganglia, Sympathetic / Q000033:anatomy & histology* / Q000378:metabolism; D008198:Lymph Nodes / Q000033:anatomy & histology / Q000294:innervation / Q000378:metabolism; D009434:Neural Pathways / Q000033:anatomy & histology / Q000378:metabolism; D009474:Neurons / Q000378:metabolism; D052248:Nitric Oxide Synthase Type I / Q000378:metabolism; D014066:Palatine Tonsil / Q000033:anatomy & histology / Q000294:innervation* / Q000378:metabolism; D009418:S100 Proteins / Q000378:metabolism; D012756:Sheep / Q000033:anatomy & histology*; D013116:Spinal Cord / Q000033:anatomy & histology / Q000378:metabolism; D013373:Substance P / Q000378:metabolism; D014446:Tyrosine 3-Monooxygenase / Q000378:metabolism
D009418:S100 Proteins; D013373:Substance P; D052248:Nitric Oxide Synthase Type I; D014446:Tyrosine 3-Monooxygenase; D004299:Dopamine beta-Hydroxylase; D015740:Calcitonin Gene-Related Peptide