DIXDC1 co-localizes and interacts with gamma-tubulin in HEK293 cells.
DIXDC1 is a Dishevelled-Axin (DIX) domain-containing protein involved in neural development and Wnt signaling pathway. Besides the DIX domain, DIXDC1 also contains a coiled-coil domain (MTH domain), which is a common feature of centrosomal proteins. We have demonstrated that exogenously expressed GFP-tag fused DIXDC1 co-localize with gamma-tubulin both at interphase and mitotic phase in HEK293 cells. By immunostaining with anti-DIXDC1 and anti-gamma-tubulin antibody, endogenous DIXDC1 was also co-localized with gamma-tubulin at the centrosomes in HEK293 cells. We confirmed this interaction of DIXDC1 with gamma-tubulin by co-immunoprecipitation. The findings suggest that DIXDC1 might play an important role in chromosome segregation and cell cycle regulation.
Department of Brain Protection and Plasticity Research, Beijing Institute of Basic Medical Sciences, Beijing, PR China.
Yanrui
Y
Wu
Xiaotang
X
Jing
Xin
X
Ma
Yan
Y
Wu
Xuefeng
X
Ding
Wenhong
W
Fan
Ming
M
Fan
D002460:Cell Line; D018385:Centrosome / Q000378:metabolism; D020090:Chromosome Segregation; D006801:Humans; D047468:Immunoprecipitation; D007399:Interphase; D047908:Intracellular Signaling Peptides and Proteins / Q000378:metabolism*; D008840:Microfilament Proteins / Q000378:metabolism*; D008938:Mitosis; D015398:Signal Transduction; D014404:Tubulin / Q000378:metabolism*
C513335:DIXDC1 protein, human; D047908:Intracellular Signaling Peptides and Proteins; D008840:Microfilament Proteins; D014404:Tubulin