HIV-1-inhibiting activity of the essential oil of Ridolfia segetum and Oenanthe crocata.
The essential oils of Ridolfia segetum (L.) Moris and Oenanthe crocata L. (Apiaceae), collected in Sardinia (Italy), have been assayed for two enzyme-associated activities of the HIV-1 reverse transcriptase (RT): RNA-dependent DNA polymerase (RDDP) activity and ribonuclease H (RNase H) activity. In biochemical assays, the essential oils inhibited HIV-1 RT RDDP activity in a dose-dependent manner, while they were inactive towards RNase H activity. Furthermore, the oils were cytotoxic towards K (562) cell replication. GC-MS analysis of the essential oils obtained by steam distillation of the aerial parts showed that the main components of R. segetum were alpha-phellandrene, alpha-terpinolene, beta-phellandrene, and dillapiol and those of O. crocata were sabinene, TRANS-beta-ocimene, CIS-beta-ocimene, and beta-pinene.
Bicchi
Carlo
C
Dipartimento di Scienza e Tecnologia del Farmaco University of Torino, Torino, Italy.
Rubiolo
Patrizia
P
Ballero
Mauro
M
Sanna
Cinzia
C
Matteodo
Maura
M
Esposito
Francesca
F
Zinzula
Luca
L
Tramontano
Enzo
E
D019380:Anti-HIV Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D019661:Apiaceae / Q000737:chemistry*; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D005591:Chemical Fractionation; D008401:Gas Chromatography-Mass Spectrometry; D015497:HIV-1 / Q000187:drug effects* / Q000201:enzymology; D006801:Humans; D031050:Oenanthe / Q000737:chemistry*; D009822:Oils, Volatile / Q000737:chemistry / Q000494:pharmacology*; D018894:Reverse Transcriptase Inhibitors / Q000494:pharmacology*; D016914:Ribonuclease H / Q000378:metabolism
D019380:Anti-HIV Agents; D009822:Oils, Volatile; D018894:Reverse Transcriptase Inhibitors; D016914:Ribonuclease H