Quantitative analysis of tumor size in a murine model of retinoblastoma.
Murine models can provide valuable insight into mechanisms of tumorigenesis. Tumor size is often used to assess the impact of genetic insult or therapeutic treatment, usually using in vivo imaging of advanced tumors. We now describe a highly sensitive method to quantify tumor volume in a mouse model of retinoblastoma, from the earliest stages of tumor initiation to large, advanced tumors. This methodology combines immunohistochemistry, digital slide scanning and computer image analysis, and can be applied to quantitatively assess and characterize early tumor development in other models.
Department of Applied Molecular Oncology, Princess Margaret Hospital/Ontario Cancer Institute, Toronto, Ontario, Canada.
Helen
H
Dimaras
Mellone N
MN
Marchong
Brenda L
BL
Gallie
D000818:Animals; D000957:Antigens, Viral, Tumor; D003941:Diagnostic Techniques, Ophthalmological; D004195:Disease Models, Animal; D007091:Image Processing, Computer-Assisted / Q000379:methods; D007150:Immunohistochemistry / Q000379:methods; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D019572:Retinal Neoplasms / Q000473:pathology*; D012175:Retinoblastoma / Q000473:pathology*; D016160:Retinoblastoma Protein
D000957:Antigens, Viral, Tumor; D016160:Retinoblastoma Protein