Accumulation of stress-related proteins within the glomeruli of the rat olfactory bulb following damage to olfactory receptor neurons.
The expression of stress-responsive proteins, such as nestin and a 27-kDa heat-shock protein (HSP27), was immunohistochemically examined in order to demonstrate glial responses in the rat olfactory bulb following sensory deprivation. At 3 days to 1 week after sensory deprivation, numerous nestin-expressing cells appeared within the glomerulus of the olfactory bulb. These cells were regarded as reactive astrocytes since they were immunoreactive for glial fibrillary acidic protein and showed hypertrophic features. The glomeruli, in which nestin-immunoreactive astrocytes were localized, were filled with degenerating terminals of olfactory receptor neurons and migrated microglia. A small population of nestin-immunoreactive cells was positive for a proliferating cell marker, Ki67 (8.0-9.7% at 3 days; 3.1 - 5.0% at 1 week). At 3 weeks, nestin-immunoreactive astrocytes were occasionally detected. At 6 weeks, when the olfactory receptor neurons had completely recovered, no nestin-immunoreactive astrocytes were detected. HSP 27 was also expressed within the glomerular astrocytes and showed a similar spatiotemporal expression pattern to nestin. The present study suggests that reactive astrocytes may be involved in axonal regeneration and synaptic remodeling in the olfactory system, through the recapitulation of developmentally regulated proteins, such as nestin and HSP27.
Department of Anatomy and Cell Biology, Graduate School of Medical Sciences, Kyushu University, Maidashi, Fukuoka, Japan. hirata@anat1.med.kyushu-u.ac.jp
Kazuho
K
Hirata
Takaaki
T
Kanemaru
Motozumi
M
Minohara
Akinobu
A
Togo
Jun-ichi
J
Kira
D000818:Animals; D001253:Astrocytes / Q000737:chemistry / Q000648:ultrastructure; D015415:Biomarkers / Q000032:analysis*; D055551:HSP27 Heat-Shock Proteins / Q000032:analysis; D007150:Immunohistochemistry; D007381:Intermediate Filament Proteins / Q000032:analysis; D019394:Ki-67 Antigen / Q000032:analysis*; D008297:Male; D008855:Microscopy, Electron, Scanning; D009419:Nerve Tissue Proteins / Q000032:analysis; D064231:Nestin; D009830:Olfactory Bulb / Q000737:chemistry*; D018034:Olfactory Receptor Neurons / Q000737:chemistry* / Q000473:pathology; D051381:Rats; D017208:Rats, Wistar
D015415:Biomarkers; D055551:HSP27 Heat-Shock Proteins; D007381:Intermediate Filament Proteins; D019394:Ki-67 Antigen; D009419:Nerve Tissue Proteins; C577486:Nes protein, rat; D064231:Nestin