Comparing corn types for differences in cell wall characteristics and p-coumaroylation of lignin.
This study was undertaken to compare cell wall characteristics including levels of p-coumarate (pCA) and lignin in corn (Zea mays L.) types. Five different types of corn, four commercial and Teosinte, were grown in the greenhouse in individual pots. For each corn type replicate stems were harvested at tassel emergence. Tissues for cell wall analysis were harvested from stems (separated into rind and pith tissues) and roots. Stem cell wall characteristics across the different corn types were similar for total neutral sugars, total uronosyls, lignin, and phenolic acids. However, the neutral sugar composition of root cell walls was markedly different, with high levels of galactose and arabinose. Levels of pCA in the different tissues ranged from 13.8 to 33.1 mg g(-1) of CW depending upon the type of tissue. There was no evidence that pCA was incorporated into cell walls attached to arabinoxylans. Lignin levels were similar within a given tissue, with pith ranging from 86.1 to 132.0 mg g(-1) of CW, rind from 178.4 to 236.6 mg g(-1) of CW, and roots from 216.5 to 242.6 mg g(-1) of CW. The higher values for lignins in root tissue may be due to suberin remaining in the acid-insoluble residue, forming Klason lignins. With the exception of root tissues, higher pCA levels accompanied higher lignin levels. This may indicate a potential role of pCA aiding lignin formation in corn cell walls during the lignification process.
Hatfield
Ronald D
RD
Agricultural Research Service, US Department of Agriculture, US Dairy Forage Research Center, 1925 Linden Drive, Madison, Wisconsin 53706, USA. rdhatfie@wisc.edu
Chaptman
Ann K
AK
D002241:Carbohydrates / Q000032:analysis; D002473:Cell Wall / Q000737:chemistry*; D003373:Coumaric Acids / Q000032:analysis* / Q000378:metabolism; D008031:Lignin / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D018517:Plant Roots / Q000648:ultrastructure; D018547:Plant Stems / Q000648:ultrastructure; D011422:Propionates; D014990:Xylans / Q000032:analysis / Q000737:chemistry; D003313:Zea mays / Q000648:ultrastructure*
D002241:Carbohydrates; D003373:Coumaric Acids; D011422:Propionates; D014990:Xylans; D008031:Lignin; C085118:arabinoxylan; C495469:p-coumaric acid