Protective role of myricetin on markers of oxidative stress in human erythrocytes subjected to oxidative stress.
The protective effect of myricetin against tert-butylhydroperoxide (t-BHP) induced oxidative stress in human erythrocytes was investigated. Incubating erythrocytes with t-BHP (10(-5) M) caused development of oxidative stress, as evidenced by significant (p < 0.05) increase in erythrocyte malondialdedyde (MDA) and protein carbonyl content, and decrease in intracellular reduced glutathione (GSH), membrane sulphydryl (-SH) groups. Incubation of erythrocytes with myricetin, simultaneously with t-BHP, protected the erythrocytes from oxidative stress, an effect which was dose-dependent. The results demonstrate that myricetin attenuates t-BHP induced oxidative damage, suggesting that supplementation of diet with myricetin/myricetin rich food may be beneficial in all pathological conditions where the antioxidant system of the body is overwhelmed.
Department of Biochemistry, University of Allahabad, Allahabad-211002, India.
Kanti Bhooshan
KB
Pandey
Neetu
N
Mishra
Syed Ibrahim
SI
Rizvi
D015415:Biomarkers / Q000378:metabolism*; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D004912:Erythrocytes / Q000187:drug effects* / Q000378:metabolism*; D005419:Flavonoids / Q000737:chemistry*; D006801:Humans; D015394:Molecular Structure; D018384:Oxidative Stress; D020122:tert-Butylhydroperoxide / Q000633:toxicity
D015415:Biomarkers; D005419:Flavonoids; C040015:myricetin; D020122:tert-Butylhydroperoxide