Three-dimensional bioprinting of rat embryonic neural cells.
We present a direct cell printing technique to pattern neural cells in a three-dimensional (3D) multilayered collagen gel. A layer of collagen precursor was printed to provide a scaffold for the cells, and the rat embryonic neurons and astrocytes were subsequently printed on the layer. A solution of sodium bicarbonate was applied to the cell containing collagen layer as nebulized aerosols, which allowed the gelation of the collagen. This process was repeated layer-by-layer to construct the 3D cell-hydrogel composites. Upon characterizing the relationship between printing resolutions and the growth of printed neural cells, single/multiple layers of neural cell-hydrogel composites were constructed and cultured. The on-demand capability to print neural cells in a multilayered hydrogel scaffold offers flexibility in generating artificial 3D neural tissue composites.
Department of Radiology, Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts 02115, USA.
Wonhye
W
Lee
Jason
J
Pinckney
Vivian
V
Lee
Jong-Hwan
JH
Lee
Krisztina
K
Fischer
Samuel
S
Polio
Je-Kyun
JK
Park
Seung-Schik
SS
Yoo
D000818:Animals; D001187:Artificial Organs; D001672:Biocompatible Materials; D002449:Cell Aggregation / Q000187:drug effects / Q000502:physiology; D018929:Cell Culture Techniques; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000502:physiology; D002478:Cells, Cultured; D003094:Collagen / Q000494:pharmacology; D003470:Culture Media / Q000737:chemistry / Q000494:pharmacology; D005109:Extracellular Matrix / Q000737:chemistry; D020100:Hydrogels / Q000494:pharmacology; D009474:Neurons / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D009924:Organ Culture Techniques; D011327:Printing / Q000379:methods*; D051381:Rats; D018874:Spheroids, Cellular / Q000166:cytology / Q000502:physiology; D013234:Stem Cells / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000639:trends*
D001672:Biocompatible Materials; D003470:Culture Media; D020100:Hydrogels; D003094:Collagen