Fabrication of self-assembled oligophenylethynylenethiol monolayer for electrochemical glucose biosensor.
An electrochemical glucose biosensor was developed using a gold (Au) electrode, which was composed of self-assembled oligophenylethynylenethiol monolayer and glucose oxidase (GOx) structure. Oligophenylethynylenethiol was used as a chemical linker for the immobilization of GOx on Au electrode, which facilitates the transfer of electron produced by enzyme reaction to the Au electrode. The electrical property of self-assembled oligophenylethynylenethiol monolayer was investigated by using cyclic voltammetry (CV). The formation of self-assembled oligophenylethynylenethiol monolayer and GOx layer on Au surface was verified by using atomic force microscopy (AFM) and surface plasmon resonance (SPR). The electrochemical glucose biosensor exhibited a linear relationship between target concentration and oxidation current in the range of 2-30mM and its detection limit was 2mM.
Department of Chemical & Biomolecular Engineering, Sogang University, Seoul 121-742, Korea.
Seul-Ki
SK
Jung
Mi-Ok
MO
Namgung
Se-Young
SY
Oh
Byung-Keun
BK
Oh
D000480:Alkynes / Q000378:metabolism; D015374:Biosensing Techniques / Q000379:methods*; D004560:Electricity; D004987:Ethers / Q000378:metabolism; D005947:Glucose / Q000032:analysis*; D005949:Glucose Oxidase / Q000378:metabolism; D006046:Gold / Q000737:chemistry*; D018625:Microscopy, Atomic Force; D011485:Protein Binding; D020349:Surface Plasmon Resonance
D000480:Alkynes; D004987:Ethers; C436384:phenyleneethynylene; D006046:Gold; D005949:Glucose Oxidase; D005947:Glucose