DNA content of hepatocyte and erythrocyte nuclei of the spined loach (Cobitis taenia L.) and its polyploid forms.
We analyzed the DNA content of hepatocyte and erythrocyte nuclei of the spined loach Cobitis taenia (diploid) and its allopolyploid forms. Twenty triploid females and one tetraploid were used. At least 20,000 hepatocyte and erythrocyte nuclei were acquired and analyzed by flow cytometry. C. taenia erythrocyte nuclei contain 3.15 +/- 0.21 pg of DNA and the hepatocyte nuclei 4.45 +/- 0.46 pg of DNA. Triploid Cobitis have 5.08 +/- 0.41 pg of DNA in erythrocyte nuclei and 6.11 +/- 0.40 pg of DNA in hepatocyte nuclei, whereas the tetraploid erythrocyte and hepatocyte nuclei contained 6.60 and 7.40 pg of DNA, respectively. In general, the DNA contents correlate positively with the ploidy level of the fish investigated. The DNA content variation in the hepatocyte and erythrocyte nuclei may be due to differences in extent of chromatin condensation, which is more pronounced in the erythrocyte than hepatocyte nuclei, or to the several orders of ploidy that occur in the parenchymal liver cells.
Juchno
Dorota
D
Department of Zoology, University of Warmia and Mazury, 5 Oczapowskiego st, 10-718, Olsztyn, Poland.
Lackowska
Bozena
B
Department of Cancer Pathology, Oncology Center, 11 Garncarska st, 31-115, Krakow, Poland.
Boron
Alicja
A
Department of Zoology, University of Warmia and Mazury, 5 Oczapowskiego st, 10-718, Olsztyn, Poland.
Kilarski
Wincenty
W
Department of Cytology and Histology, Institute of Zoology, Jagiellonian University, 6 Ingardena st, 30-060, Krakow, Poland. wincenty.kilarski@uj.edu.pl.
D000818:Animals; D002467:Cell Nucleus / Q000235:genetics*; D003531:Cypriniformes / Q000235:genetics*; D004247:DNA / Q000032:analysis*; D004912:Erythrocytes / Q000737:chemistry*; D005260:Female; D005434:Flow Cytometry; D022781:Hepatocytes / Q000737:chemistry*; D011044:Poland; D011123:Polyploidy; D045483:Rivers
D004247:DNA