Reduced levels of miR-34a in neuroblastoma are not caused by mutations in the TP53 binding site.
Neuroblastoma (NB) is the most common extracranial solid tumor in children below the age of 5 years. miR-34a, located in chromosome band 1p36, has been recently implicated as a tumor suppressor gene in NB. In addition, it has been shown that miR-34a is activated by TP53 by binding to a TP53 binding site upstream to the mature miR-34a. We studied NB tumors from 57 patients for miR-34a expression levels, 1p status, mutations in the TP53 coding region and mutations of the TP53 binding site. Reduced expression levels of miR-34a were identified in tumors harboring 1p36.3 Loss (P = 0.028). No mutations were identified in the coding region of TP53, or in the TP53 binding site. Thus, mutations in the binding site are not an additional mechanism for the inactivation of miR-34a in NB. Other regulatory mechanisms controlling miR-34a expression and its relationship to TP53 should be further explored.
Feinberg-Gorenshtein
Galina
G
Molecular Oncology, Felsenstein Medical Research Center, Petah Tikva, Israel.
Avigad
Smadar
S
Jeison
Marta
M
Halevy-Berco
Gili
G
Mardoukh
Jacques
J
Luria
Drorit
D
Ash
Shifra
S
Steinberg
Ran
R
Weizman
Avraham
A
Yaniv
Isaac
I
D001665:Binding Sites; D002648:Child; D002675:Child, Preschool; D003627:Data Interpretation, Statistical; D020869:Gene Expression Profiling; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D009154:Mutation; D009447:Neuroblastoma / Q000235:genetics / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D016159:Tumor Suppressor Protein p53 / Q000235:genetics* / Q000378:metabolism
C522425:MIRN34 microRNA, human; D035683:MicroRNAs; C495901:TP53 protein, human; D016159:Tumor Suppressor Protein p53