Controlling the selection stringency of phage display using a microfluidic device.
We report the utilization of microfluidic technology to phage selection and demonstrate that accurate control of washing stringency in our microfluidic magnetic separator (MMS) directly impacts the diversity of isolated peptide sequences. Reproducible generation of magnetic and fluidic forces allows controlled washing conditions that enable rapid convergence of selected peptide sequences. These findings may provide a foundation for the development of automated microsystems for rapid in vitro directed evolution of affinity reagents.
Liu
Yanli
Y
Neuroscience Research Institute, University of California, Santa Barbara, CA93106, USA.
Adams
Jonathan D
JD
Turner
Kelisha
K
Cochran
Frank V
FV
Gambhir
Sanjiv Sam
SS
Soh
H Tom
HT
D000595:Amino Acid Sequence; D008280:Magnetics; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation* / Q000379:methods* / Q000592:standards; D008969:Molecular Sequence Data; D019151:Peptide Library; D010455:Peptides / Q000032:analysis; D011786:Quality Control; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D019151:Peptide Library; D010455:Peptides