A method for fabricating protein patterns on the octadecyltrichlorosialne(OTS) surface through paper swabbing.
A simple protein patterning procedure was developed for fabricating clean protein patterns ranging from nanometer to sub-millimeter scale. A carboxylic acid-terminated chemical pattern was fabricated first through the local probe oxidation on the octadecyltrichlorosilane (OTS) film under 100% relative humidity at the first. After incubating the chemical pattern in a protein solution, the sample was swabbed with a piece of ChemWipe paper to selectively remove the non-specifically adsorbed protein. After the swabbing, only the specifically immobilized protein remained on the surface, forming a protein pattern on the chemical template with a very clean background. In our approach, an anti-fouling surface or vigorous rinsing is not required, which simplifies the protein patterning process. As a demonstration of the capability of this protein patterning approach, we fabricated the lysozyme and catalase patterns on the OTS surface. Both patterns were clean and bioactive.
Department of Chemistry, University of Kentucky, Rose Street, Lexington, KY 40506, USA.
Pei
P
Gao
Yuguang
Y
Cai