Rolling-circle amplification: unshared advantages in miRNA detection.
Roll with it: The quantitative analysis of specific miRNAs from biological samples is very likely to revolutionize diagnostics of human disease. A novel method for miRNA analysis employing rolling-circle amplification (RCA) can homogeneously detect miRNA, even at concentrations as low as 10 fM. The use of T4 RNA ligase 2 (T4 RnL2) at elevated temperatures enables very good discrimination of miRNAs differing by a single nucleotide.
Neubacher
Saskia
S
Humboldt-Universität zu Berlin, Institut für Chemie, Brook-Taylor-Strasse 2, 12489 Berlin, Germany. christoph.arenz@chemie.hu-berlin.de
Arenz
Christoph
C
D035683:MicroRNAs / Q000032:analysis* / Q000235:genetics / Q000378:metabolism; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D011130:RNA Ligase (ATP) / Q000378:metabolism; D044366:Transition Temperature; D014764:Viral Proteins / Q000378:metabolism
D035683:MicroRNAs; D014764:Viral Proteins; D011130:RNA Ligase (ATP); C467724:bacteriophage T4 RNA ligase 2