Biflavonoids from Araucaria angustifolia protect against DNA UV-induced damage.
Ultraviolet radiation is one of the most deleterious forms of radiation to terrestrial organisms and is involved in formation of mutagenic pyrimidine dimers and oxidized nucleotides. The biflavonoid fraction (BFF), extracted from needles of Araucaria angustifolia was capable of protecting calf thymus DNA from damage induced by UV radiation. This occurred through prevention of cyclobutane thymine dimer and 8-oxo-7,8-dihydro-2'-deoxyguanosine formation, this being quantified by high-performance liquid chromatography coupled to tandem mass spectrometry (HPLC-MS/MS) in a multiple reaction monitoring mode (MRM) and by HPLC-coulometric detection, respectively.
Departamento de Química Fundamental, Instituto de Química, Universidade de São Paulo, São Paulo, SP, Brazil, Cx Postal 26077, CEP 05599-970, Brazil. lydyama@iq.usp.br
Lydia F
LF
Yamaguchi
Massuo J
MJ
Kato
Paolo
P
Di Mascio
D000818:Animals; D044946:Biflavonoids / Q000302:isolation & purification* / Q000494:pharmacology; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D004247:DNA / Q000528:radiation effects*; D004249:DNA Damage / Q000187:drug effects*; D004563:Electrochemistry; D011740:Pyrimidine Dimers; D053719:Tandem Mass Spectrometry; D064028:Tracheophyta / Q000737:chemistry*; D014466:Ultraviolet Rays
D044946:Biflavonoids; D011740:Pyrimidine Dimers; D004247:DNA