Introduction to protein blotting.
Protein blotting is a powerful and important procedure for the immunodetection of proteins following electrophoresis, particularly proteins that are of low abundance. Since the inception of the protocol for protein transfer from an electrophoresed gel to a membrane in 1979, protein blotting has evolved greatly. The scientific community is now confronted with a variety of ways and means to carry out this transfer.
Oklahoma Medical Research Foundation, 825 NE 13th Street, OK 73104, Oklahoma City, USA. biji-kurien@omrf.org
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D015153:Blotting, Western / Q000295:instrumentation / Q000379:methods; D002021:Buffers; D003101:Collodion / Q000737:chemistry; D011145:Polyvinyls / Q000737:chemistry; D011506:Proteins / Q000032:analysis*; D005465:Fluorocarbon Polymers
D002021:Buffers; D003101:Collodion; D011145:Polyvinyls; D011506:Proteins; C024865:polyvinylidene fluoride; D005465:Fluorocarbon Polymers