Immunoblotting using radiolabeled reagents for detection.
Development of immunoblots is commonly performed using enzyme-labeled antibodies, which convert soluble substrates into insoluble colored products. A simple, rapid, and sensitive alternative method that produces low background and allows a rapid quantitative evaluation is the use of radiolabeled antibodies or protein A conjugates. Here, we describe the use of iodinated secondary antibodies for immunodetection of an autoantigen during HPLC purification.
Institute Immunology, MTZ, Carl Gustav Carus University TU Dresden, Medical Faculty, Fiedlerstrasse 42, D-01307, Dresden, Germany.
Holger
H
Bartsch
Claudia
C
Franke
Michael
M
Bachmann
D000906:Antibodies / Q000737:chemistry* / Q000276:immunology; D001324:Autoantigens / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D006367:HeLa Cells; D006801:Humans; D015151:Immunoblotting / Q000379:methods*; D018796:Immunoconjugates / Q000737:chemistry* / Q000276:immunology; D007202:Indicators and Reagents / Q000737:chemistry*; D012320:RNA Polymerase III / Q000737:chemistry; D012261:Ribonucleoproteins / Q000737:chemistry; D013205:Staphylococcal Protein A / Q000737:chemistry* / Q000276:immunology; D000097585:SS-B Antigen
D000906:Antibodies; D001324:Autoantigens; D018796:Immunoconjugates; D007202:Indicators and Reagents; D012320:RNA Polymerase III; D012261:Ribonucleoproteins; D013205:Staphylococcal Protein A; D000097585:SS-B Antigen