Characterization of oligomeric species on the aggregation pathway of human lysozyme.
The aggregation process of wild-type human lysozyme at pH3.0 and 60 degrees C has been analyzed by characterizing a series of distinct species formed on the aggregation pathway, specifically the amyloidogenic monomeric precursor protein, the oligomeric soluble prefibrillar aggregates, and the mature fibrils. Particular attention has been focused on the analysis of the structural properties of the oligomeric species, since recent studies have shown that the oligomers formed by lysozyme prior to the appearance of mature amyloid fibrils are toxic to cells. Here, soluble oligomers of human lysozyme have been analyzed by a range of techniques including binding to fluorescent probes such as thioflavin T and 1-anilino-naphthalene-8-sulfonate, Fourier transform infrared spectroscopy, and controlled proteolysis. Oligomers were isolated after 5 days of incubation of the protein and appear as spherical particles with a diameter of 8-17 nm when observed by transmission electron microscopy. Unlike the monomeric protein, oligomers have solvent-exposed hydrophobic patches able to bind the fluorescent probe 1-anilino-naphthalene-8-sulfonate. Fourier transform infrared spectroscopy spectra of oligomers are indicative of misfolded species when compared to monomeric lysozyme, with a prevalence of random structure but with significant elements of the beta-sheet structure that is characteristic of the mature fibrils. Moreover, the oligomeric lysozyme aggregates were found to be more susceptible to proteolysis with pepsin than both the monomeric protein and the mature fibrils, indicating further their less organized structure. In summary, this study shows that the soluble lysozyme oligomers are locally unfolded species that are present at low concentration during the initial phases of aggregation. The nonnative conformational features of the lysozyme molecules of which they are composed are likely to be the factors that confer on them the ability to interact inappropriately with a variety of cellular components including membranes.
Frare
Erica
E
CRIBI Biotechnology Centre, University of Padua, Padua, Italy.
Mossuto
Maria F
MF
de Laureto
Patrizia Polverino
PP
Tolin
Serena
S
Menzer
Linda
L
Dumoulin
Mireille
M
Dobson
Christopher M
CM
Fontana
Angelo
A
D000817:Anilino Naphthalenesulfonates / Q000737:chemistry; D001704:Biopolymers / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D005456:Fluorescent Dyes / Q000737:chemistry; D006801:Humans; D046529:Microscopy, Electron, Transmission; D009113:Muramidase / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D017550:Spectroscopy, Fourier Transform Infrared
D000817:Anilino Naphthalenesulfonates; D001704:Biopolymers; D005456:Fluorescent Dyes; C027132:1-anilino-8-naphthalenesulfonate; D009113:Muramidase