Involvement of hypothalamic glutamate in cisplatin-induced emesis in Suncus murinus (house musk shrew).
We investigated the effect of cisplatin on glutamate release in the hypothalamus of Suncus murinus measured by brain microdialysis. Dialysis samples were collected every 20 min for 1 h before and 3 h after the cisplatin (30 mg/kg, i.p.) administration with the animals also being observed for the development of emesis. Cisplatin increased glutamate levels within 1 h and this was closely associated with the occurrence of emesis. Pretreatment with the 5-HT(3)-receptor antagonist ondansetron (2 mg/kg, i.p.) inhibited both the emesis and the increased glutamate levels. These results suggest that hypothalamic glutamate is involved in cisplatin-induced emesis in Suncus murinus.
Department of Medical Physics and Engineering, Division of Health Sciences, Graduate School of Medicine, Osaka University, Suita, Osaka, Japan. kouichi@sahs.med.osaka-u.ac.jp
Kouichi
K
Yamamoto
Sze Wa
SW
Chan
John A
JA
Rudd
Ge
G
Lin
Keiko
K
Asano
Atsushi
A
Yamatodani
D000818:Animals; D000932:Antiemetics / Q000627:therapeutic use; D000970:Antineoplastic Agents; D001921:Brain / Q000473:pathology; D002945:Cisplatin; D005260:Female; D018698:Glutamic Acid / Q000502:physiology*; D007031:Hypothalamus / Q000187:drug effects / Q000378:metabolism*; D007150:Immunohistochemistry; D017551:Microdialysis; D017294:Ondansetron / Q000627:therapeutic use; D012788:Shrews / Q000502:physiology*; D014839:Vomiting / Q000139:chemically induced* / Q000188:drug therapy / Q000503:physiopathology*
D000932:Antiemetics; D000970:Antineoplastic Agents; D018698:Glutamic Acid; D017294:Ondansetron; D002945:Cisplatin