[Effects on activity of mouse NK cells by inhibitory Ly49 receptors blockade].
To observe the effects on antitumor activity of mouse NK cells and the mixed lymphocyte reaction with blocking the interaction between the inhibitory Ly49 receptor on mouse NK cell and MHC-I molecules on target cells with monoclonal antibody.



NK cells were isolated from splenocytes of C57BL/6J mouse with immunomagnetic method. YAC-1 and M1 cells were used as target cells, the cytotoxicity of NK cells was examined with mixed lymphocyte reaction before and after treatment of anti-Ly49 monoclonal antibody.



The cytotoxicity of NK cells to M1 cell was significantly augmented (10.7% +/- 0.5% compared with 84.4 % +/- 2.9%), and the clone-forming capacity of M1 cell was inhibited remarkably (8.2 % +/- 2.2% compared with 94.0% +/- 3.3%) with the increase of concentration of the antibody (0 approximate, equals 60 microg/ml) (P <0.05). The difference of cytotoxicity to YAC-1 cells was only found between 0 microg/ml and 60 microg/ml groups (P <0.05). In mixed lymphocyte reaction, after pretreated with antibody, the proliferation of splenocytes of BALB/C mouse was inhibited (0.398 +/-0.025 compared with 0.128 +/- 0.014), and statistical difference was observed only between antibody groups of 0 microg/ml and 60 microg/ml (P<0.05).



Blocking the inhibitory Ly49 receptor on NK cells with monoclonal antibody augments the cytotoxicity to target cells.
Xu
Yun
Y
College of Medicine, Zhejiang University, Hangzhou 310003, China.
Huang
He
H
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology / Q000494:pharmacology*; D049109:Cell Proliferation; D002478:Cells, Cultured; D005260:Female; D005805:Genes, MHC Class I; D007694:Killer Cells, Natural / Q000276:immunology*; D007959:Lymphocyte Culture Test, Mixed; D008223:Lymphoma / Q000276:immunology* / Q000473:pathology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D055650:NK Cell Lectin-Like Receptor Subfamily A / Q000276:immunology*; D013154:Spleen / Q000166:cytology / Q000276:immunology
D000911:Antibodies, Monoclonal; C527088:Klra1 protein, mouse; D055650:NK Cell Lectin-Like Receptor Subfamily A