Identification of acute phase proteins and assays applicable in nondomesticated mammals.
The serum concentration of acute phase proteins (APPs) increases dramatically in response to inflammation and tissue injury. APPs are clinically useful in a range of domesticated mammals; however, knowledge is limited in nondomesticated mammals. The detective ability of two assays for each of three potential APPs--serum amyloid A (SAA), C-reactive protein (CRP), and haptoglobin (Hp)--was evaluated in eight species. For SAA, a turbidimetric immunoassay (TIA) demonstrated significant detective abilities in the Asian elephant (Elaphas maximus), impala (Aepyceros melampus), musk ox (Ovibos moschatus), and chimpanzee (Pan troglodytes), as did an SAA enzyme-linked immunosorbent assay (ELISA) in the impala. For CRP, both TIA and ELISA had significant detective abilities in the chimpanzee. For Hp, a colorimetric assay demonstrated significant detective abilities in impala, musk ox, sitatunga (Tragelaphus spekeii), and chimpanzee, as did the Hp ELISA in the impala, musk ox, and sitatunga. In conclusion, these results suggest that assays for detection of relevant APPs in several nondomesticated animals are available.
Bertelsen
Mads F
MF
Center for Zoo and Wild Animal Health, Copenhagen Zoo, Roskildevej 38, DK-2000 Frederiksberg, Denmark. mfb@zoo.dk
Kjelgaard-Hansen
Mads
M
Grøndahl
Carsten
C
Heegaard
Peter M H
PM
Jacobsen
Stine
S
D000209:Acute-Phase Proteins / Q000032:analysis*; D000818:Animals; D000835:Animals, Wild / Q000097:blood*; D000836:Animals, Zoo / Q000097:blood*; D015415:Biomarkers / Q000097:blood; D002097:C-Reactive Protein / Q000032:analysis; D003124:Colorimetry / Q000379:methods / Q000662:veterinary; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000662:veterinary; D006242:Haptoglobins / Q000032:analysis; D007249:Inflammation / Q000097:blood / Q000175:diagnosis / Q000662:veterinary*; D009391:Nephelometry and Turbidimetry; D012016:Reference Values; D012680:Sensitivity and Specificity; D000685:Serum Amyloid A Protein / Q000032:analysis; D013045:Species Specificity
D000209:Acute-Phase Proteins; D015415:Biomarkers; D006242:Haptoglobins; D000685:Serum Amyloid A Protein; D002097:C-Reactive Protein