Experimental autoimmune encephalomyelitis in the rat.
There are several diverse rat models of experimental autoimmune encephalomyelitis (EAE) that can be used to investigate the pathogenesis and regulation of autoimmunity against CNS myelin. The disease course of these models ranges from an acute monophasic disease with limited demyelination to a chronic relapsing or chronic progressive course marked by severe demyelination. These models enable the study of encephalitogenic T cells and demyelinating antibody specific for major neuroantigens such as myelin basic protein (MBP), myelin oligodendrocyte glycoprotein (MOG), or proteolipid protein (PLP), among other important CNS autoantigens. Overall, this unit provides an overview of common methods for induction of active and passive EAE, assessment and analysis of clinical disease, preparation and purification of myelin basic protein, and derivation of neuroantigen-specific rat T cell lines. This unit also provides a brief discussion of the basic characteristics of these models.
Department of Microbiology and Immunology, East Carolina University, Brody School of Medicine, Greenville, North Carolina.
Mark
M
Mannie
Wayne State University, Detroit, Michigan.
Robert H
RH
Swanborg
Henry Ford Community College, Dearborn, Michigan.
Jolie A
JA
Stepaniak
D019264:Adoptive Transfer; D000818:Animals; D001324:Autoantigens / Q000276:immunology; D015551:Autoimmunity; D035843:Biomedical Research / Q000379:methods*; D002457:Cell Extracts / Q000008:administration & dosage / Q000276:immunology; D003711:Demyelinating Diseases; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000209:etiology / Q000276:immunology / Q000473:pathology; D006168:Guinea Pigs; D007114:Immunization; D004676:Myelin Basic Protein / Q000276:immunology* / Q000302:isolation & purification; D009185:Myelin Proteins; D018991:Myelin Proteolipid Protein / Q000276:immunology / Q000302:isolation & purification; D018992:Myelin-Associated Glycoprotein / Q000276:immunology / Q000302:isolation & purification; D063308:Myelin-Oligodendrocyte Glycoprotein; D051381:Rats; D011919:Rats, Inbred Strains; D013116:Spinal Cord / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D037182:T-Cell Antigen Receptor Specificity / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology* / Q000473:pathology
D001324:Autoantigens; D002457:Cell Extracts; C571328:Mog protein, rat; D004676:Myelin Basic Protein; D009185:Myelin Proteins; D018991:Myelin Proteolipid Protein; D018992:Myelin-Associated Glycoprotein; D063308:Myelin-Oligodendrocyte Glycoprotein