Mannose-exposing myeloid leukemia cells detected by the sCAR-PPA fusion protein.
Altered glycosylation may be a hallmark of malignant transformation and cancer progression. In the work described, a specific mannose-binding lectin, Pinellia pedatisecta agglutinin (PPA), was genetically fused with the extracellular domain of coxsackie-adenovirus receptor (CAR) to generate the soluble CAR (sCAR)-PPA fusion protein. The adenoviral transduction of acute myeloid leukemia (AML) cell lines Kasumi-1 and HL-60 was increased by sCAR-PPA, indicating that a fraction of AML cells exposing mannose residues was detected by PPA. However, sCAR-PPA did not increase the adenoviral infection of KG-1 cells, suggesting the mannose exposure of AML cells may be cell type specific. Furthermore, the infectious efficiency of Ad-EGFP in chronic myeloid leukemia cell line K562 was significantly increased by sCAR-PPA as well. We, herein, report that PPA recognized a fraction of myeloid leukemia cells showing mannose-exposing phenotype. The sCAR-PPA fusion protein combined with the adenoviral vector system may provide a useful tool for investigating myeloid leukemia cells exposing mannose residues and further elucidating the role of these cells in the leukemia development.
Xin Yuan Institute of Medicine and Biotechnology, College of Life Sciences, Zhejiang Sci-Tech University, Hangzhou, People's Republic of China.
Gong Chu
GC
Li
Na
N
Li
Yan Hong
YH
Zhang
Xin
X
Li
Yi Gang
YG
Wang
Xin Yuan
XY
Liu
Wen Bin
WB
Qian
Xiao Chuan
XC
Liu
D000373:Agglutinins / Q000235:genetics; D045744:Cell Line, Tumor; D062727:Coxsackie and Adenovirus Receptor-Like Membrane Protein; D006801:Humans; D007951:Leukemia, Myeloid / Q000175:diagnosis / Q000473:pathology*; D008358:Mannose / Q000378:metabolism*; D037601:Mannose-Binding Lectin; D030021:Pinellia / Q000235:genetics; D011991:Receptors, Virus / Q000235:genetics; D011993:Recombinant Fusion Proteins
D000373:Agglutinins; C568771:CLMP protein, human; D062727:Coxsackie and Adenovirus Receptor-Like Membrane Protein; D037601:Mannose-Binding Lectin; D011991:Receptors, Virus; D011993:Recombinant Fusion Proteins; D008358:Mannose