Nuclease resistant methylphosphonate-DNA/LNA chimeric oligonucleotides.
Synthesis of chimeric 9-mer oligonucleotides containing methylphosphonate-linkages and locked nucleic acid (LNA) monomers, their binding affinity towards complementary DNA and RNA, and their 3'-exonucleolytic stability are described. The obtained methylphosphonate-DNA/LNA chimeric oligonucleotides display similarly high RNA affinity and RNA selectivity as a corresponding 9-mer DNA/LNA chimeric oligonucleotide, but much higher resistance towards 3'-exonucleolytic degradation.
Nucleic Acid Center, Department of Physics and Chemistry, University of Southern Denmark, Odense M, Denmark.
Koji
K
Nagahama
Rakesh N
RN
Veedu
Jesper
J
Wengel
D000818:Animals; D001483:Base Sequence; D004247:DNA / Q000737:chemistry*; D005092:Exonucleases / Q000378:metabolism; D009841:Oligonucleotides / Q000138:chemical synthesis / Q000737:chemistry* / Q000378:metabolism; D016376:Oligonucleotides, Antisense / Q000138:chemical synthesis / Q000737:chemistry* / Q000378:metabolism; D010755:Organophosphates / Q000737:chemistry*; D010727:Phosphoric Diester Hydrolases / Q000378:metabolism*; D012313:RNA / Q000737:chemistry; D012911:Snakes
D009841:Oligonucleotides; D016376:Oligonucleotides, Antisense; D010755:Organophosphates; C477371:locked nucleic acid; D012313:RNA; C027265:methylphosphate; D004247:DNA; D005092:Exonucleases; D010727:Phosphoric Diester Hydrolases