Nucleic acid pool preparation and characterization.
Random sequence nucleic acid pools can be used in a variety of applications, including the selection of functional nucleic acids such as protein binding sites, aptamers, and ribozymes. while the design, synthesis, and purification of pools is relatively straightforward, keeping track of the size and complexity of a nucleic acid pool can sometimes task even an experienced researcher. The following protocol takes the reader through the steps necessary for the preparation of a pool of known complexity.
Piasecki
Shawn K
SK
Department of Chemistry and Biochemistry, University of Texas at Austin, Austin, TX, USA.
Hall
Bradley
B
Ellington
Andrew D
AD
D052157:Aptamers, Nucleotide / Q000138:chemical synthesis / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D017931:DNA Primers / Q000138:chemical synthesis / Q000235:genetics / Q000302:isolation & purification; D009696:Nucleic Acids / Q000138:chemical synthesis* / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D016133:Polymerase Chain Reaction; D052156:SELEX Aptamer Technique / Q000379:methods*
D052157:Aptamers, Nucleotide; D017931:DNA Primers; D009696:Nucleic Acids