Local and systemic cytokine responses during larval penetration in cattle experimentally infested with Hypoderma lineatum (Diptera: Oestridae).
Local and systemic cytokine responses were studied in 3 groups of cattle, with 4 animals each, experimentally infested with Hypoderma lineatum (De Villers) first instars (L1). The first group was undergoing a primary infestation (G-1), the second group was undergoing a secondary infestation (G-2) and the third group was infested for their third consecutive year (G-3). Cattle were infested with 25 L1 deposited on the skin. Blood and skin samples were taken at 0, 6, 12, 48, 96 and 144h post-infestation (h.p.i.). Interleukin 10 (IL-10), IL-4 and interferon gamma (IFN-gamma) production was studied by immunohistochemistry and sandwich ELISAs. IL-4(+) cells showed a significant increase at 6h.p.i. in both reinfested groups (G-2 and G-3) when compared with G-1. In all groups the number of IL-4(+) cells decreased significantly at 48h.p.i. IL-10(+) cells increased in G-1 at 6 and 48h.p.i., whereas in both reinfested groups increased at 12h.p.i. with a peak at 48h.p.i. IFN-gamma(+) cells showed a significant increment at 6h.p.i. in all groups, followed by a rapid descent at 12 (G-1 and G-2) and 48h.p.i. (G-3). Penetration of the skin by H. lineatum did not have any significant effect on IFN-gamma serum concentrations and, except for IL-10 there were no correlation between local production and serum concentrations of cytokines. The increase of both Th1 (IFN-gamma) and Th2-type cytokines (IL-4 and IL-10) indicates that bovine T-cell response during the first phases of the infestation by H. lineatum is apparently a Th0 response.
Dacal
V
V
Departamento de Patología Animal: Sanidad Animal, Facultad de Veterinaria, Universidad de Santiago de Compostela, 27071 Lugo, Spain.
Colwell
D D
DD
López
C
C
Pérez
V
V
Vázquez
L
L
Cienfuegos
S
S
Díaz
P
P
Morrondo
P
P
Díez-Baños
P
P
Panadero
R
R
D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000276:immunology*; D016207:Cytokines / Q000032:analysis / Q000096:biosynthesis*; D004175:Diptera / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D007150:Immunohistochemistry; D007371:Interferon-gamma / Q000096:biosynthesis; D016753:Interleukin-10 / Q000096:biosynthesis; D015847:Interleukin-4 / Q000096:biosynthesis; D007814:Larva; D009198:Myiasis / Q000276:immunology / Q000662:veterinary*
D016207:Cytokines; D016753:Interleukin-10; D015847:Interleukin-4; D007371:Interferon-gamma