Hemolymph amino acid variations following behavioral and genetic changes in individual Drosophila larvae.
This study investigated the effect of different sampling environments on hemolymph amino acid content of individual Drosophila melanogaster larvae. Hemolymph was collected from individual third instar larvae under cold-anesthetized, awake, and stress conditions. Qualitative and quantitative hemolymph amino acid analyses were performed via capillary electrophoresis with laser-induced fluorescence detection. The hemolymph amino acid concentrations, particularly arginine, glutamate, and taurine, changed significantly depending on the prior-to-sample-collection environments. Hemolymph amino acid analyses of six different Drosophila genotypes including two control genotypes and four mutant alleles were also carried out. Two mutant genotypes with over and under expression of a putative cystine-glutamate exchanger subunit were significantly different from each other with respect to their hemolymph glutamate, glycine, lysine, and taurine levels. Hemolymph amino acid analyses of stressed larvae of two control and two mutant genotypes indicated that behavior-related hemolymph chemical changes are also genotype dependent.
Piyankarage
Sujeewa C
SC
Department of Chemistry MC 111, University of Illinois at Chicago, 845 West Taylor Street, Chicago, IL 60607, USA.
Augustin
Hrvoje
H
Featherstone
David E
DE
Shippy
Scott A
SA
D000483:Alleles; D026905:Amino Acid Transport Systems / Q000235:genetics; D000596:Amino Acids / Q000097:blood* / Q000235:genetics; D000758:Anesthesia; D000818:Animals; D001522:Behavior, Animal / Q000502:physiology*; D029721:Drosophila Proteins / Q000235:genetics; D004331:Drosophila melanogaster / Q000235:genetics* / Q000502:physiology*; D019075:Electrophoresis, Capillary; D014644:Genetic Variation; D005838:Genotype; D006458:Hemolymph / Q000737:chemistry*; D007814:Larva; D009154:Mutation; D013312:Stress, Physiological / Q000235:genetics
D026905:Amino Acid Transport Systems; D000596:Amino Acids; D029721:Drosophila Proteins; C542118:JhI-21 protein, Drosophila