Effect of splenectomy and autologous spleen transplantation on the serum platelet-activating factor acetylhydrolase (PAF-AH) activity and acute phase response (APR) in a porcine model.
The aim of this study was to evaluate the inflammatory response after total splenectomy and spleen autotransplantation in a porcine model by measuring serum platelet-activating factor acetylhydrolase activity, C-reactive protein and albumin concentrations. Nineteen piglets were used in the experiment. After induction of anesthesia, animals were randomly divided into three groups: sham-operation with spleens intact (n = 6), total splenectomy (n = 6), and splenic autotransplantation (n = 7) with small fragments of the spleen autotransplanted into the greater omentum. The blood samples were taken just before surgery and on day 1st, 5th, 12th, 26th and 40th postoperatively. PAF-AH activity, CRP and albumin concentrations were assayed in the sera. After total splenectomy, PAF-AH activity was significantly increased on day 5th, while there was no significant increase after spleen autotransplantation or the sham-operation. CRP was significantly increased after surgery in all experimental groups. Albumin was significantly decreased after surgery from day 5th until day 40th in splenectomized and autotransplanted pigs. Increased PAF-AH activity after splenectomy and spleen autotransplantation might be attributed to inflammatory conditions due to the loss of splenic tissue and trauma. Time-course increase of CRP, in all groups after surgery suggests post-injury inflammatory response due to tissue lesion during operation.
Turk
Romana
R
Department of Pathophysiology, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia. rturk@vef.hr
Vnuk
Drazen
D
Svetina
Ante
A
Flegar-Mestrić
Zlata
Z
Flegar-Mestri
Zlata
Z
Robić
Mirna
M
Robi
Mirna
M
Turk
Nenad
N
Staresina
Vilim
V
Rumenjak
Vlatko
V
Juretić
Dubravka
D
Jureti
Dubravka
D
D043203:1-Alkyl-2-acetylglycerophosphocholine Esterase / Q000097:blood*; D000210:Acute-Phase Reaction / Q000201:enzymology*; D000818:Animals; D002097:C-Reactive Protein / Q000032:analysis; D005260:Female; D007700:Kinetics; D008297:Male; D023421:Models, Animal; D016377:Organ Transplantation / Q000379:methods; D012709:Serum Albumin / Q000032:analysis; D013154:Spleen / Q000502:physiology* / Q000637:transplantation*; D013156:Splenectomy / Q000009:adverse effects / Q000379:methods; D013552:Swine; D014182:Transplantation, Autologous; D016896:Treatment Outcome
D012709:Serum Albumin; D002097:C-Reactive Protein; D043203:1-Alkyl-2-acetylglycerophosphocholine Esterase