Ultrahigh spacing tissue arrays for screening hybridoma by immunohistochemistry.
We present a technology to make ultrahigh spacing tissue arrays (USTA) which facilitates screening hybridoma by immunohistochemistry, results in easier performance of staining and image analysis, minimal sample mixed-up and less bookkeeping efforts. 32 array cores are designated a 4 x 8 microarray, leaving 4 mm spacing between two adjacent array elements. After separated each array spot with PAP pen, one USTA is sufficient for testing of supernatants from 32 different hybridomas.
Department of Immunology, Fourth Military Medical University, 17 Changle West Road, Xi'an 710032, PR China.
Chaojun
C
Song
Fuli
F
Wang
Liang
L
Jin
Lihua
L
Chen
Weijun
W
Qin
Angang
A
Yang
Kun
K
Yang
Boquan
B
Jin
D000818:Animals; D006801:Humans; D006825:Hybridomas / Q000166:cytology*; D007150:Immunohistochemistry / Q000379:methods*; D051379:Mice; D046228:Microarray Analysis; D016610:Tissue Embedding / Q000379:methods*