High throughput substrate phage display for protease profiling.
The interplay between a protease and its substrates is controlled at many different levels, including coexpression, colocalization, binding driven by ancillary contacts, and the presence of natural inhibitors. Here we focus on the most basic parameter that guides substrate recognition by a protease, the recognition specificity at the catalytic cleft. An understanding of this substrate specificity can be used to predict the putative substrates of a protease, to design protease activated imaging agents, and to initiate the design of active site inhibitors. Our group has characterized protease specificities of several matrix metalloproteinases using substrate phage display. Recently, we have adapted this method to a semiautomated platform that includes several high-throughput steps. The semiautomated platform allows one to obtain an order of magnitude more data, thus permitting precise comparisons among related proteases to define their functional distinctions.
Ratnikov
Boris
B
Center on Proteolytic Pathways, Burnham Institute for Medical Research, La Jolla, CA, USA.
Cieplak
Piotr
P
Smith
Jeffrey W
JW
D020134:Catalytic Domain; D010447:Peptide Hydrolases / Q000737:chemistry / Q000378:metabolism*; D019151:Peptide Library; D013379:Substrate Specificity
D019151:Peptide Library; D010447:Peptide Hydrolases