Cytoprotective effect of lignans from Forsythia suspensa against peroxynitrite-induced LLC-PK1 cell damage.
There is mounting evidence that peroxynitrite (ONOO(-)) is closely related to the pathogenesis of various diseases. As a pharmacological strategy aimed at preventing ONOO(-)-mediated toxicity, the protective activity of Forsythia suspensa (Thunb.) Vahl (Oleaceae) against ONOO(-)-induced cellular damage was investigated and its active components identified. After bioactivity-guided fractionation of its methylene chloride fraction, two tetrahydrofurofuran lignans were isolated, namely phillygenin and 8-hydroxypinoresinol. The protective effects of these lignans against ONOO(-)-induced cell death were evaluated using renal epithelial cell LLC-PK1. Phillygenin and 8-hydroxypinoresinol significantly reduced the cell injury by 3-morpholinosydnonimine (SIN-1), a ONOO(-) generator. The hydroxy substituents on the phenyl moieties may contribute to the antioxidant activities of these lignans. These results suggest that phillygenin and 8-hydroxypinoresinol may be useful for the therapeutic or preventive applications in treating ONOO(-)-related diseases.
Institute of Chinese Minority Traditional Medicine, Minzu University of China, Beijing 100081, China.
Xiang Lan
XL
Piao
Eun Jung
EJ
Cho
Eun June
EJ
Cho
Moon Hee
MH
Jang
Jian
J
Cui
D000818:Animals; D019610:Cytoprotection; D031657:Forsythia / Q000737:chemistry; D018374:LLC-PK1 Cells; D017705:Lignans / Q000494:pharmacology*; D015394:Molecular Structure; D008981:Molsidomine / Q000031:analogs & derivatives / Q000494:pharmacology; D030421:Peroxynitrous Acid / Q000494:pharmacology*; D010936:Plant Extracts / Q000494:pharmacology*; D013552:Swine
C542293:8-hydroxypinoresinol; D017705:Lignans; D010936:Plant Extracts; D030421:Peroxynitrous Acid; C002385:linsidomine; C542294:phillygenin; D008981:Molsidomine