[Lifetime diagnosis of trichinosis in experimentally infected laboratory animals].
The authors propose a new method for lifetime diagnosis (LTD) of experimental invasion of Trichinella spiralis in migratory and muscular phases, which is based on thin-layer microscopy of minor samples of skeletal muscles (weighing 1-1.5 mg) on gradually increased impulse manual compression. The LTD method permits the monitoring of the most important follow-up qualitative and quantitative invasion parameters in each infected animal in normalcy and under the action of specific therapeutic and preventive agents (the onset and termination of a migratory phrase, the elimination time of intestinal Trichinella after infection, the number, sizes, morphological features, viability, and motor activity of nonincapsulated and incapsulated muscular Trichinella larvae of different age). The most important feature of LTD is its possibility of detecting and determining the viability of neonatal T. spiralis larvae. LTD enhances the informative value, reduces the costs of and makes experimental studies of the basic and applied problems of trichinosis shorter.
Repina
E A
EA
Kovalenko
F P
FP
Kukhaleva
I V
IV
D000818:Animals; D004195:Disease Models, Animal; D051379:Mice; D008853:Microscopy / Q000379:methods*; D018482:Muscle, Skeletal / Q000469:parasitology* / Q000648:ultrastructure*; D017160:Trichinella spiralis / Q000302:isolation & purification / Q000648:ultrastructure*; D014235:Trichinellosis / Q000175:diagnosis* / Q000473:pathology