Cytotoxic aaptamines from Malaysian Aaptos aaptos.
In a preliminary screen, Aaptos aaptos showed significant cytotoxic activity towards a panel of cell lines and was thus subjected to bioassay-guided isolation of the bioactive constituents. In addition to the known aaptamine, two new derivatives of the alkaloid were isolated from the bioactive chloroform fraction of the crude methanolic extract. Detailed analysis by NMR and mass spectroscopy enabled their identification to be 3-(phenethylamino)demethyl(oxy)aaptamine and 3-(isopentylamino)demethyl(oxy) aaptamine. The cytotoxic activities of the three alkaloids were further evaluated against CEM-SS cells.
Laboratory of Natural Products, Institute of Bioscience, University Putra Malaysia, 43400 Serdang, Selangor, Malaysia. khozirah@yahoo.com.my
Khozirah
K
Shaari
Kee Cheng
KC
Ling
Zalilawati Mat
ZM
Rashid
Tan Pei
TP
Jean
Faridah
F
Abas
Salahudin Mohd
SM
Raof
Zurina
Z
Zainal
Nordin Hj
NH
Lajis
Habsah
H
Mohamad
Abdul Manaf
AM
Ali
D000470:Alkaloids / Q000302:isolation & purification* / Q000494:pharmacology; D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000302:isolation & purification* / Q000494:pharmacology; D045744:Cell Line, Tumor; D004354:Drug Screening Assays, Antitumor; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D008296:Malaysia; D013058:Mass Spectrometry; D009287:Naphthyridines / Q000302:isolation & purification* / Q000494:pharmacology; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology; D011161:Porifera / Q000737:chemistry*
C541247:3-(isopentylamino)demethyl(oxy)aaptamine; C541246:3-(phenethylamino)demethyl(oxy)aaptamine; D000470:Alkaloids; D000972:Antineoplastic Agents, Phytogenic; D009287:Naphthyridines; D010936:Plant Extracts; C043986:aaptamine