Ion mobility spectrometry as a detector for molecular imprinted polymer separation and metronidazole determination in pharmaceutical and human serum samples.
Application of ion mobility spectrometry (IMS) as the detection technique for a separation method based on molecular imprinted polymer (MIP) was investigated and evaluated for the first time. On the basis of the results obtained in this work, the MIP-IMS system can be used as a powerful technique for separation, preconcentration, and detection of the metronidazole drug in pharmaceutical and human serum samples. The method is exhaustively validated in terms of sensitivity, selectivity, recovery, reproducibility, and column capacity. The linear dynamic range of 0.05-70.00 microg/mL was obtained for the determination of metronidazole with IMS. The recovery of analyzed drug was calculated to be above 89%, and the relative standard deviation (RSD) was lower than 6% for all experiments. Various real samples were analyzed with the coupled techniques, and the results obtained revealed the efficient cleanup of the samples using MIP separation before the analysis by IMS as a detection technique.
Department of Chemistry, Isfahan University of Technology, Isfahan 84156-83111, Iran. jafari@cc.iut.ac.ir
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Jafari
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Rezaei
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Zaker
D002138:Calibration; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008795:Metronidazole / Q000032:analysis* / Q000097:blood* / Q000302:isolation & purification; D054802:Molecular Imprinting; D004364:Pharmaceutical Preparations / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D052616:Solid Phase Extraction; D012997:Solvents / Q000737:chemistry; D013057:Spectrum Analysis / Q000379:methods*; D013607:Tablets; D013997:Time Factors
D004364:Pharmaceutical Preparations; D011108:Polymers; D012997:Solvents; D013607:Tablets; D008795:Metronidazole