[Cloning and prokaryotic expression of lipid storage droplet protein 5 gene].
OBJECTIVE
To clone the Lipid storage droplet protein 5 (LSDP5) and express it in E.coli.


METHODS
LSDP5 gene was amplified by PCR from the mouse liver cDNA library, and inserted into pMD18-T vector and sequenced. After analysis of hydrophobicity and secondary structure using software, a fragment of LSDP5 was subcloned into GST fusion expression vector, and the recombinant expression plasmid pEGX-LSDP5 was constructed and expressed in E.coli BL21.


RESULTS
LSDP5 gene was obtained and its sequence was identical with that reported in GenBank. The E.coli containing pEGX-LSDP5 could express a 40 kD protein after induction of IPTG.


CONCLUSIONS
A confirmed LSDP5 gene is cloned and expressed in E.coli successfully, which lay the foundation for the related research of this gene.
Liu
Fang
F
Department of Pathology, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China. liqing@fmmu.edu.cn
Li
Qing
Q
Ye
Jing
J
Zhang
Jing
J
Zhang
Li-ying
LY
Li
Fan-fan
FF
Li
Hang
H
Gu
Yu
Y
D000818:Animals; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics*; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000235:genetics; D015870:Gene Expression; D057927:Hydrophobic and Hydrophilic Interactions; D008099:Liver / Q000378:metabolism; D051379:Mice; D000070797:Perilipin-5; D011506:Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism*; D017422:Sequence Analysis, DNA
D018076:DNA, Complementary; D000070797:Perilipin-5; C511805:Plin5 protein, mouse; D011506:Proteins; D011994:Recombinant Proteins