Rapid detection of the poly-gamma-D-glutamic acid capsular antigen of Bacillus anthracis by latex agglutination.
Latex agglutination has been used to detect capsular polysaccharides from a variety of bacteria in body fluids. A latex agglutination assay was constructed for detection of the poly-gamma-D-glutamic acid (gammaDPGA) capsular polypeptide of Bacillus anthracis in serum from animal models of pulmonary anthrax. The assay was able to detect gammaDPGA in serum from infected animals at concentrations of 100 to 200 ng/mL.
AuCoin
David P
DP
Department of Microbiology and Immunology, University of Nevada School of Medicine, Reno, 89557, USA. daucoin@medicine.nevada.edu
Sutherland
Marjorie D
MD
Percival
Ann L
AL
Lyons
C Rick
CR
Lovchik
Julie A
JA
Kozel
Thomas R
TR
D000818:Animals; D000881:Anthrax / Q000175:diagnosis*; D000942:Antigens, Bacterial / Q000032:analysis* / Q000276:immunology; D001408:Bacillus anthracis / Q000737:chemistry / Q000276:immunology / Q000302:isolation & purification*; D016667:Bacterial Capsules / Q000737:chemistry* / Q000276:immunology; D007841:Latex Fixation Tests / Q000379:methods; D051379:Mice; D008807:Mice, Inbred BALB C; D011099:Polyglutamic Acid / Q000031:analogs & derivatives* / Q000032:analysis / Q000276:immunology; D044967:Serum / Q000737:chemistry
D000942:Antigens, Bacterial; C511775:poly(gamma-glutamic acid); D011099:Polyglutamic Acid