Development of loop-mediated isothermal amplification (LAMP) as a diagnostic tool of toxoplasmosis.
Infection with Toxoplasma gondii is one of the most common parasitic infections in humans and other warm-blooded animals. This paper describes the development of loop-mediated isothermal amplification (LAMP) specific to the single-copy gene SAG1 as a diagnostic tool of toxoplasmosis. A set of primers, composed of outer primers, inner primers and loop primers was designed from a published sequence data (GeneBank Acc. no. AY651825). Experiments showed that when LAMP was applied to sample organs, amplification absolutely required the loop primers to complete. SAG1-based LAMP turned out to be very sensitive, exhibiting a degree of sensitivity higher than the conventional PCR. LAMP is a convenient and sensitive diagnostic tool for routine health control of toxoplasmosis.
Krasteva
D
D
UMR 5119, CNRS-UM2, Université Montpellier II, 34095 Montpellier Cedex 05, France.
Toubiana
M
M
Hartati
Sri
S
Kusumawati
Asmarani
A
Dubremetz
J F
JF
Widada
J Sri
JS
D000818:Animals; D000953:Antigens, Protozoan; D051379:Mice; D021141:Nucleic Acid Amplification Techniques / Q000379:methods / Q000662:veterinary*; D016133:Polymerase Chain Reaction / Q000379:methods; D015800:Protozoan Proteins; D012680:Sensitivity and Specificity; D013997:Time Factors; D014122:Toxoplasma / Q000302:isolation & purification; D014123:Toxoplasmosis / Q000175:diagnosis* / Q000469:parasitology
D000953:Antigens, Protozoan; D015800:Protozoan Proteins; C047168:SAG1 antigen, Toxoplasma