Development and media regulate alternative splicing of a methyltransferase pre-mRNA in Monascus pilosus.
Two alternatively spliced mRNAs (d- and l-MpLaeA) of a methyltransferase gene (MpLaeA) were identified from Monascus pilosus IFO4520 and its mutant MK-1. Alternative splicing of the MpLaeA pre-mRNA occurred in the 5'-untranslated region (5'-UTR). The alternative splicing patterns of MpLaeA were regulated by the fungal growth stage and the principal nutrients: that is, the short l-MpLaeA mRNA was a constitutive transcript at all growth stages and different carbon or nitrogen sources, but the glutamate and NaNO(3) as main nitrogen source could up-regulate the long d-MpLaeA mRNA form. The long spliced 5'-UTR of d-MpLaeA blocked GFP expression in Escherichia coli , suggesting that d-MpLaeA mRNA was an ineffective spliced mRNA. Down-regulation of MpLaeA by transgenic antisense d-MpLaeA cDNA resulted in decreasing synthesis of monacolin K in M. pilosus. This suggested that the alternative splicing of MpLaeA mRNA might regulate the synthesis of monacolin K.
South China Botanical Garden, Chinese Academy of Sciences, 723 Xingkelu, Guangzhou 510650, China. zhangmy@scbg.ac.cn
Ming-Yong
MY
Zhang
Tsuyoshi
T
Miyake
D020121:5' Untranslated Regions / Q000235:genetics; D017398:Alternative Splicing / Q000235:genetics*; D000595:Amino Acid Sequence; D002244:Carbon; D003470:Culture Media; D018076:DNA, Complementary / Q000302:isolation & purification; D004926:Escherichia coli / Q000235:genetics; D015870:Gene Expression / Q000187:drug effects; D018698:Glutamic Acid; D049452:Green Fluorescent Proteins / Q000235:genetics; D008780:Methyltransferases / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D032741:Monascus / Q000235:genetics* / Q000254:growth & development*; D009566:Nitrates; D009584:Nitrogen; D012322:RNA Precursors / Q000235:genetics*
D020121:5' Untranslated Regions; D003470:Culture Media; D018076:DNA, Complementary; D009566:Nitrates; D012322:RNA Precursors; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins; D018698:Glutamic Acid; D002244:Carbon; C031618:sodium nitrate; D008780:Methyltransferases; D009584:Nitrogen