[The comparative determination mutations in mtDNA of irradiated mice using mismatch-specific endonuclease and TTGE-technique].
We defined the mutations in mtDNA of X-irradiated mice brair using mismatch-specific endonuclease (CEL I-nuclease method) and by temporal temperature gradient gel electrophoresis (TTGE-technique). The comparison of the received by both methods, allows to conclude, that CEL I-nuclease method gives more qualitative results, than TTGE-technique. Moreover, CEL I-nuclease method is more sensitive, in contrast with TTGE-technique. The CEL I-nuclease method allows simultaneously to conduct the analysis of big amount of sample DNA, to get the reproducible results. It does not require complex equipment and economical. The analysis of mutations in mtDNA of brain of X-irradiated mice by CEL I-nuclease method has shown, that the amount of mutant copies mtDNA is essentially reduced (in 2-3 times) with 8 up to 28 days of the post-radiation period. However the amount mtDNA copies in brain tissue of the irradiated animals is remains during all post radiation time without change though lower, concerning given control group. The results permit the suggestion that mutant mtDNA copies are eliminated from the tissues of irradiated animals in the post-radiation period.
S A
SA
Abdullaev
N A
NA
Guliaeva
V G
VG
Bezlepkin
A I
AI
Gaziev
D000818:Animals; D020137:Base Pair Mismatch; D001921:Brain / Q000378:metabolism* / Q000528:radiation effects; D004252:DNA Mutational Analysis / Q000379:methods*; D004272:DNA, Mitochondrial / Q000235:genetics* / Q000528:radiation effects*; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D004720:Endonucleases / Q000737:chemistry*; D016158:Genes, p53 / Q000235:genetics / Q000528:radiation effects; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009692:Nucleic Acid Heteroduplexes / Q000032:analysis; D016133:Polymerase Chain Reaction / Q000379:methods; D013696:Temperature
D004272:DNA, Mitochondrial; D009692:Nucleic Acid Heteroduplexes; D004720:Endonucleases; C406922:CEL I nuclease