Scavenging of superoxide anion radical and hydroxyl radical by novel thiazolyl-thiazolidine-2,4-dione compounds.
The antioxidant behavior of a series of new synthesized substituted thiazolyl-thiazolidine-2,4-dione compounds (TZDs) was examined using chemiluminescence and electron paramagnetic resonance spin trapping techniques. 5,5-Dimethyl-1-pyrroline-N-oxide (DMPO) was used as the spin trap. The reactivity of TZDs with superoxide anion radical (O2(*-)) and hydroxyl radical (HO(*)) was evaluated using potassium superoxide/18-crown-6 ether dissolved in dimethylsulfoxide, and the Fenton-like reaction (Fe(2+) + H2O2), respectively. The results showed that TZDs efficiently inhibited light emission from the O2(*-) generating system at a concentration of 0.05-1 mmol L(-1) (5-94% reductions were found at 1 mmol L(-1) concentration). The TZD compounds showed inhibition of HO(*)-dependent DMPO-OH spin adduct formation from DMPO (the amplitude decrease ranged from 8 to 82% at 1 mmol L(-1) concentration). The findings showed that examined TZDs had effective activities as radical scavengers.
Ankara University, Faculty of Pharmacy, Department of Pharmaceutical Chemistry, 06100 Tandoğan, Ankara, Turkey.
Oya
O
Bozdağ-Dündar
Selen
S
Gürkan
Hassan Y
HY
Aboul-Enein
Irena
I
Kruk
Aleksandra
A
Kładna
D000975:Antioxidants / Q000737:chemistry*; D004578:Electron Spin Resonance Spectroscopy; D016166:Free Radical Scavengers / Q000737:chemistry*; D017665:Hydroxyl Radical / Q000737:chemistry*; D008163:Luminescent Measurements; D015394:Molecular Structure; D012680:Sensitivity and Specificity; D013237:Stereoisomerism; D013481:Superoxides / Q000737:chemistry*; D013844:Thiazoles / Q000737:chemistry*; D045162:Thiazolidinediones / Q000737:chemistry*
D000975:Antioxidants; D016166:Free Radical Scavengers; D013844:Thiazoles; D045162:Thiazolidinediones; D013481:Superoxides; D017665:Hydroxyl Radical