Oligonucleotide array-based detection and genotyping of mollicutes (Acholeplasma, Mycoplasma, and Ureaplasma).
An oligonucleotide array was developed to detect and genotype mollicutes based on the internal transcribed spacer (ITS) sequence. The results of the assay were compared with those of a PCR-RFLP assay. The proposed oligonucleotide array containing 5 genus- and 23 species-specific probes was able to detect Mycoplasma species, including M. penetrans and M. spermatophilum, that were not detected by the PCRRFLP assay. Therefore, the results demonstrated that the proposed oligonucleotide array was effective for the detection and discrimination of 23 species, including an acholeplasma, 21 mycoplasmas, and a ureaplasma, and showed promise as a countermeasure to ensure that biological products are safe and of good quality.
Jang
Hyunjung
H
Institute for Genomic Medicine, GeneIn Co., Ltd, Busan 617-030, Korea.
Kim
Hyomyeung
H
Kang
Byeongchul
B
Kim
Cheolmin
C
Park
Heekyung
H
D000127:Acholeplasma / Q000235:genetics; D054458:Amplified Fragment Length Polymorphism Analysis; D004269:DNA, Bacterial / Q000302:isolation & purification; D021903:DNA, Ribosomal Spacer / Q000302:isolation & purification; D020459:Genes, rRNA; D009174:Mycoplasma / Q000235:genetics; D020411:Oligonucleotide Array Sequence Analysis; D010802:Phylogeny; D011786:Quality Control; D021521:Ribotyping / Q000379:methods*; D012680:Sensitivity and Specificity; D017422:Sequence Analysis, DNA; D013045:Species Specificity; D008973:Tenericutes / Q000235:genetics*; D014509:Ureaplasma / Q000235:genetics
D004269:DNA, Bacterial; D021903:DNA, Ribosomal Spacer