Surface plasmon resonance-based detection of ladder-shaped polyethers by inhibition detection method.
Ladder-shaped polyether (LSP) compounds represented by brevetoxins and ciguatoxins were largely discovered in association with seafood poisoning. Thus, a quick quantification method for LSPs is potentially important. We examined a surface plasmon resonance method using desulfated-yessotoxin (dsYTX) immobilized on a sensor chip and phosphodiesterase PDEII in a inhibition detection mode. Yessotoxin, brevetoxin B and synthetic LSP derivatives showed clear inhibition against PDEII binding to the immobilized dsYTX, by which their half inhibitory concentrations were successfully estimated. This inhibition method appeared to be superior in specificity to direct binding assays where binding proteins to LSP was immobilized on a sensor chip.
Mouri
Ryota
R
Department of Chemistry, Osaka University, Machikaneyama, Toyonaka, Japan.
Oishi
Tohru
T
Torikai
Kohei
K
Ujihara
Satoru
S
Matsumori
Nobuaki
N
Murata
Michio
M
Oshima
Yasukatsu
Y
D019921:Biotinylation; D002922:Ciguatoxins / Q000032:analysis / Q000737:chemistry; D054678:Cyclic Nucleotide Phosphodiesterases, Type 2 / Q000037:antagonists & inhibitors / Q000378:metabolism*; D004987:Ethers / Q000032:analysis / Q000737:chemistry*; D008387:Marine Toxins / Q000032:analysis / Q000737:chemistry; D008978:Mollusk Venoms; D035964:Oxocins / Q000032:analysis / Q000737:chemistry; D020349:Surface Plasmon Resonance / Q000379:methods*; D000095702:Polyether Toxins
D002922:Ciguatoxins; D054678:Cyclic Nucleotide Phosphodiesterases, Type 2; D004987:Ethers; D008387:Marine Toxins; D008978:Mollusk Venoms; D035964:Oxocins; C053342:brevetoxin; D000095702:Polyether Toxins; C066632:yessotoxin