Application of real time PCR for diagnosis of Swine Dysentery.
Evaluation of a genetic diagnostic technique using real time PCR of Swine Dysentery (SD) was performed using nox primers. Culture, ordinary PCR and real time PCR were compared in this experiment. Sixty-seven specimens from pigs with clinical signs of SD brought to a slaughterhouse in Shibaura, Tokyo, were used. B. hyodysenteriae was isolated from 49 of the pigs, was detected by ordinary PCR in 49 of the pigs and was detected by real time PCR in 54 of the pigs. Furthermore, we were able to determine the numbers of B. hyodysenteriae cells in all positive specimens by real time PCR. The rapid diagnostic technique established in this experiment was useful for detection of B. hyodysenteriae because it was more effective than ordinary PCR and culture.
Health and Safety Division, Bureau of Social Welfare and Public Health, Tokyo Metropolitan Government, Tokyo, Japan. Satoru_Akase@member.metro.tokyo.jp
Satoru
S
Akase
Yumi
Y
Uchitani
Yoshiko
Y
Sohmura
Keikichi
K
Tatsuta
Kenji
K
Sadamasu
Yoshikazu
Y
Adachi
D000818:Animals; D017401:Brachyspira hyodysenteriae / Q000235:genetics; D004269:DNA, Bacterial / Q000302:isolation & purification; D004403:Dysentery / Q000662:veterinary*; D016905:Gram-Negative Bacterial Infections / Q000175:diagnosis / Q000382:microbiology / Q000662:veterinary*; D016133:Polymerase Chain Reaction / Q000662:veterinary*; D013552:Swine; D013553:Swine Diseases / Q000175:diagnosis*
D004269:DNA, Bacterial