Possibilities and limits of X-ray microtomography for in vivo and ex vivo detection of vascular calcifications.
In the present paper, vascular calcifications due to chronic renal failure in rats are studied by X-ray microtomography (micro-CT). Although micro-CT is traditionally used as an imaging technique, a quantitative analysis of data obtained by in vivo and ex vivo micro-CT is described and discussed. By comparison with traditional destructive methods, such as histomorphometry and atomic absorption, the detection limits for calcium were determined in living rats and in extracted aortas. micro-CT proved to be an effective non-invasive imaging technique allowing non-destructive quantification of ectopic calcifications.
Postnov
A A
AA
Laboratory of Microtomography, University of Antwerp, Universiteitsplein 1, Campus Drie Eiken, Antwerp, Belgium. andrei.postnov@ua.ac.be
D'Haese
P C
PC
Neven
E
E
De Clerck
N M
NM
Persy
V P
VP
D000225:Adenine; D000818:Animals; D001011:Aorta / Q000378:metabolism / Q000473:pathology; D001018:Aortic Diseases / Q000000981:diagnostic imaging* / Q000209:etiology / Q000378:metabolism / Q000473:pathology; D001027:Aortography / Q000379:methods*; D002114:Calcinosis / Q000000981:diagnostic imaging* / Q000209:etiology / Q000378:metabolism / Q000473:pathology; D002118:Calcium / Q000378:metabolism*; D004195:Disease Models, Animal; D007676:Kidney Failure, Chronic / Q000139:chemically induced / Q000150:complications* / Q000000981:diagnostic imaging; D008297:Male; D011237:Predictive Value of Tests; D051381:Rats; D017208:Rats, Wistar; D015203:Reproducibility of Results; D013054:Spectrophotometry, Atomic; D013194:Staining and Labeling / Q000379:methods; D013997:Time Factors; D055114:X-Ray Microtomography
D000225:Adenine; D002118:Calcium