Variation in the levels of inflammatory cytokines depending on ischemic time: effects on respiratory variables.
OBJECTIVE
We sought to evaluate the association between ischemic times, cytokines-interleukin (IL)-6, IL-1b, tumor necrosis factor-alpha, sIL-2r, IL-8, and IL-10-and alterations in gaseous exchange.


METHODS
This prospective study of 42 orthotopic liver transplantation (OLT) recipients examined ischemic times and respiratory variables measured as alterations in intrapulmonary shunt and in the Po(2)/Fio(2) ratio. Centrifuged blood samples were frozen at -80 degrees C for storage. The Inmulite-One system (Euro/Dpc, Gwynedd, UK) was used to determine the concentration of cytokines. For statistical analysis, we used the Pearson correlation coefficient.


RESULTS
The average cold ischemic time was 478 minutes (range, 35-929) and warm ischemic time was 69.58 minutes (range, 20-180). The warm ischemic time affected the degree of shunt at the end of the operation (P < .027) and the levels of IL-10 (P < .018) and IL-6 (P < .000). The final degree of shunting and IL-10 (P < .044) showed a correlation. The cold ischemic time affected IL-1 (P < .046) and IL-8 levels (P < .023). The reperfusion syndrome was correlated with the final levels of IL-10 (P < .064) and of IL-8 (P < .066).


CONCLUSIONS
Warm and cold ischemic times affect the final cytokine levels and the degree of intrapulmonary shunt.
Duran
J Arranz
JA
Liver Transplant Unit, Hospital Universitario NS de Candelaria, Tenerife, Spain. javierarranz@telefonica.net
González
A Arteaga
AA
García
D Domínguez
DD
Falcón
R Calvo
RC
Pereda
P Sanz
PS
Alvárez
S Méndez
SM
Cobaleda
I García
IG
Jaime
A Aguirre
AA
González
I Arteaga
IA
Bosque
A Varona
AV
Gómez
M Barrera
MB
Benítez de Lugo
A Soriano
AS
D016207:Cytokines / Q000097:blood*; D006801:Humans; D007249:Inflammation / Q000097:blood; D007375:Interleukin-1 / Q000097:blood; D016753:Interleukin-10 / Q000097:blood; D015850:Interleukin-6 / Q000097:blood; D016209:Interleukin-8 / Q000097:blood; D007511:Ischemia / Q000097:blood / Q000276:immunology*; D008102:Liver Circulation / Q000276:immunology; D016031:Liver Transplantation / Q000276:immunology*; D010100:Oxygen / Q000097:blood; D010101:Oxygen Consumption; D010313:Partial Pressure; D011169:Portal Vein / Q000502:physiology; D015424:Reperfusion; D015427:Reperfusion Injury / Q000097:blood / Q000276:immunology*
D016207:Cytokines; D007375:Interleukin-1; D015850:Interleukin-6; D016209:Interleukin-8; D016753:Interleukin-10; D010100:Oxygen