Molecular mechanism of transcriptional cascade initiated by the EvgS/EvgA system in Escherichia coli K-12.
Using an EvgS-active mutant (evgS1) in combination with gene deletions, we clarified the molecular mechanism of the transcriptional cascade of acid resistance and multidrug resistance genes initiated by the EvgS/EvgA two-component system in Escherichia coli, followed by sequential induction of the transcriptional regulators, YdeO and GadE. Overexpression of EvgA, the response regulator of the EvgS/EvgA system, is known to induce the expression of a number of acid resistance and multidrug resistance genes, in which the EvgA-YdeO-GadE circuit is involved, but the role of the sensor EvgS in this circuit has remained unsolved. Our results suggest that the transcriptional cascade initiated by the EvgS/EvgA system in fact functions for acid and drug resistance in E. coli.
Department of Bioscience, Graduate School of Agriculture, Kinki University, 3327-204 Nakamachi, Nara 631-8505, Japan.
Junji
J
Itou
Yoko
Y
Eguchi
Ryutaro
R
Utsumi
D000143:Acids / Q000494:pharmacology; D001483:Base Sequence; D018432:Drug Resistance, Multiple / Q000235:genetics; D048168:Escherichia coli K12 / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism* / Q000502:physiology; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism*; D005798:Genes, Bacterial / Q000235:genetics; D008969:Molecular Sequence Data; D009154:Mutation; D011494:Protein Kinases / Q000378:metabolism*; D015398:Signal Transduction; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D014158:Transcription, Genetic; D015533:Transcriptional Activation; D015854:Up-Regulation
D000143:Acids; D029968:Escherichia coli Proteins; C489314:EvgA protein, E coli; C465223:EvgS protein, E coli; C479562:GadE protein, E coli; D014157:Transcription Factors; C513431:YdeO protein, E coli; D011494:Protein Kinases