Effect of L-Arginine and L-NAME treatments on polymorphonuclear leukocytes and mononuclear cells influx during tumor growth.
OBJECTIVE
Evaluate polymorphonuclear leukocytes (PMN's) and mononuclear cells (MN's) involvement in the Ehrlich s solid tumor (ET) growth.


METHODS
90 Swiss mice were inoculated with 10(7) tumor cells (sc), distributed in three groups and treated once a day, via intraperitoneal (ip), with 0.1ml of diluent, L-Arginine (20mg/Kg) or L-NAME (20mg/Kg). After 7, 15 and 30 days of treatment, ten animals of each group were euthanized, the tumor mass was removed, processed and fixed for HE. Later, a morphometric analysis of the total area, parenchyma, necrosis, tumor stroma and PMN's leukocytes and MN's cells influx was performed.


RESULTS
The L-Arginine treatment increased PMN's influx in the initial stage, whereas L-NAME reduced it. Our data suggests that NO effect on PMN's migration is dose-dependent. On the other hand, the MN s cells influx was reduced by L-NAME treatment at all evaluated periods and at the same periods an increase in tumor growth was observed.


CONCLUSIONS
At initial stages of tumor implantation, both PMN's leukocytes and MN's cells act together to control ET development.
Reis
Olívia Teixeira Gomes
OT
Center on Health Sciences, Biology Department, USC, Bauru - SP, Brazil. olivia_tgreis@terra.com.br
Raini
Juliana Crepaldi
JC
Coradi
Silvana Torossian
ST
Constantino
Dulce Helena Jardim
DH
D000818:Animals; D001120:Arginine / Q000494:pharmacology*; D002286:Carcinoma, Ehrlich Tumor / Q000473:pathology*; D004195:Disease Models, Animal; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D007963:Leukocytes, Mononuclear / Q000473:pathology* / Q000502:physiology; D008297:Male; D051379:Mice; D019331:NG-Nitroarginine Methyl Ester / Q000494:pharmacology*; D009504:Neutrophils / Q000187:drug effects* / Q000502:physiology; D019001:Nitric Oxide Synthase / Q000378:metabolism
D004791:Enzyme Inhibitors; D001120:Arginine; D019001:Nitric Oxide Synthase; D019331:NG-Nitroarginine Methyl Ester