Anti-human parvovirus B19 nonstructural protein antibodies in patients with rheumatoid arthritis.
BACKGROUND
Previous studies have reported the association between the development of NS1-specific IgG and arthropathy after the infection of human parvovirus B19 (B19). However, the role of anti-B19-NS1 IgG in RA is still unclear. This study investigated the role of anti-B19-NS1 antibody in patients with rheumatoid arthritis (RA).


METHODS
B19-VP IgM and IgG antibodies, nested PCR, B19-NS1 IgM and IgG antibodies, and anti-cyclic citrullinated peptide (CCP) antibodies were assessed by ELISA and Western blot in this study.


RESULTS
Significantly higher prevalence of B19-NS1 IgM and IgG antibodies in patients with recent B19 infection was observed as well as the higher prevalence of B19-NS1 IgM and IgG antibodies in RA patients with seronegative diagnostic patterns. However, no significant variation of both B19-NS1 IgM and IgG was detected in RA patients with different B19 diagnostic patterns. Additionally, significantly higher presence of anti-CCP IgG was observed in RA patients with B19-NS1 IgM.


CONCLUSIONS
This study suggests the possibility of anti-B19-NS1 IgM as an indicator for RA diagnosis and indicates the suspense of the higher prevalence of anti-B19-NS1 antibody in RA patients with seronegative B19 diagnostic patterns. However, these results provide clues in understanding the association of anti-B19-NS1 antibody in RA patients.
Tzang
Bor-Show
BS
Institute of Biochemistry and Biotechnology, Chung Shan Medical University, Taichung, Taiwan.
Tsai
Chun-Chou
CC
Tsay
Gregory J
GJ
Wang
Meilin
M
Sun
Yu-Shu
YS
Hsu
Tsai-Ching
TC
D000293:Adolescent; D000328:Adult; D000368:Aged; D000914:Antibodies, Viral / Q000276:immunology*; D001172:Arthritis, Rheumatoid / Q000097:blood / Q000150:complications / Q000276:immunology*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009154:Mutation / Q000235:genetics; D010322:Parvoviridae Infections / Q000097:blood / Q000150:complications / Q000175:diagnosis / Q000276:immunology*; D016732:Parvovirus B19, Human / Q000276:immunology*; D017361:Viral Nonstructural Proteins / Q000235:genetics / Q000276:immunology* / Q000378:metabolism
D000914:Antibodies, Viral; D017361:Viral Nonstructural Proteins