Spatial regulation of specific gene expression through photoactivation of RNAi.
In this study we describe the spatial regulation of RNA interference (RNAi) using an RNA-carrier protein labeled with a fluorescent dye and a light source to trigger the RNAi. We demonstrate photo-dependent gene silencing using several dyes with different excitation wavelengths. Additionally, we use light from a halogen lamp and a photomask to produce photopatterned RNAi, and laser light to trigger single-cell RNAi on cell culture plates.
Department of Bioscience and Biotechnology, Okayama University, Okayama 700-8530, Japan.
Tamaki
T
Endoh
Masahiko
M
Sisido
Takashi
T
Ohtsuki
D000818:Animals; D016466:CHO Cells; D006224:Cricetinae; D003412:Cricetulus; D004337:Drug Carriers / Q000008:administration & dosage / Q000737:chemistry; D005456:Fluorescent Dyes / Q000008:administration & dosage / Q000737:chemistry*; D015696:Gene Products, tat / Q000008:administration & dosage / Q000737:chemistry*; D007834:Lasers; D010777:Photochemistry; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000008:administration & dosage*; D011993:Recombinant Fusion Proteins / Q000008:administration & dosage / Q000737:chemistry; D017412:Ribonucleoprotein, U1 Small Nuclear / Q000008:administration & dosage / Q000737:chemistry*
D004337:Drug Carriers; D005456:Fluorescent Dyes; D015696:Gene Products, tat; D034741:RNA, Small Interfering; D011993:Recombinant Fusion Proteins; D017412:Ribonucleoprotein, U1 Small Nuclear; C075339:U1A protein