Vaccination of mice with baculovirus-infected insect cells expressing antigenic proteins.
Methods to induce antigen-specific immune responses in mice using insect cells infected with recombinant baculoviruses are described in this unit. Although this vaccine strategy has been used to generate both antibody and T cell responses, it has been more thoroughly characterized for the peptide-specific cytotoxic T cell responses. Nonspecific responses to the vaccine vehicle are controlled for by vaccinating with insect cells infected with baculoviruses encoding irrelevant antigens or no antigen. The baculovirus-infected insect cells alone are an effective immune adjuvant to elicit antigen-specific T cells. Overall, immune responses generated using this approach are similar to those generated by more conventional vaccine strategies.
University of Colorado, Denver, Colorado.
Kimberly R
KR
Jordan
National Jewish Health, Denver, Colorado.|Howard Hughes Medical Institute, Denver, Colorado.
Fran
F
Crawford
University of Colorado, Denver, Colorado.|National Jewish Health, Denver, Colorado.|Howard Hughes Medical Institute, Denver, Colorado.
John W
JW
Kappler
University of Colorado, Denver, Colorado.
Jill E
JE
Slansky
D000276:Adjuvants, Immunologic; D000818:Animals; D000941:Antigens / Q000276:immunology*; D016367:Baculoviridae / Q000235:genetics / Q000276:immunology*; D018929:Cell Culture Techniques; D020869:Gene Expression Profiling; D005805:Genes, MHC Class I / Q000276:immunology; D005818:Genetic Engineering; D007313:Insecta / Q000276:immunology* / Q000378:metabolism; D051379:Mice; D010455:Peptides / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology; D014611:Vaccination / Q000295:instrumentation / Q000379:methods*; D014612:Vaccines
D000276:Adjuvants, Immunologic; D000941:Antigens; D010455:Peptides; D014612:Vaccines