Comparison of methods to assess the enzyme accessibility and hydrolysis of pretreated lignocellulosic substrates.
Fiber size analysis, water retention value, and Simons' stain measurements were assessed for their potential to predict the susceptibility of a given substrate to enzymatic hydrolysis. Slight modifications were made to the fiber size analysis and water retention protocols to adapt these measurements to evaluate substrates for cellulolytic hydrolysis rather than pulps for papermaking. Lodgepole pine was pretreated by the steam and ethanol-organosolv processes under varying conditions. The Simons' stain procedure proved to be an effective method for indicating the potential ease of enzymatic hydrolysis of substrates pretreated by either process or when the pretreatment conditions were altered.
Department of Wood Science, Faculty of Forestry, University of British Columbia, 2424 Main Mall, Vancouver, BC, V6T, Canada.
Richard P
RP
Chandra
Shannon M
SM
Ewanick
Pablo A
PA
Chung
Kathy
K
Au-Yeung
Luis
L
Del Rio
Warren
W
Mabee
Jack N
JN
Saddler
D044602:Cellulases / Q000378:metabolism*; D006868:Hydrolysis; D008031:Lignin / Q000378:metabolism*; D028223:Pinus / Q000378:metabolism*
C036909:lignocellulose; D008031:Lignin; D044602:Cellulases