Exploring the ameliorative potential of Punica granatum in dextran sulfate sodium induced ulcerative colitis in mice.
The present study was designed to investigate the ameliorative potential of Punica granatum in dextran sulfate sodium (DSS) induced ulcerative colitis. DSS (2%) was administered orally in drinking water for 7 days to induce ulcerative colitis. The extent and severity of ulceration was analysed macroscopically, histopathologically and using a disease activity index. Myeloperoxidase (MPO), a specific marker of inflammation; histamine, a marker of mast cell degranulation; superoxide anion generation and, lipid peroxides were analysed. Administration of DSS resulted in a significant development of ulceration in the colon along with a rise in histamine, MPO activity and oxidative stress. Treatment with Punica granatum extract and its ellagic acid rich fraction (100 mg/kg and 200 mg/kg p.o.) significantly attenuated DSS-induced colonic inflammation along with attenuation of histamine, MPO and oxidative stress. The antiulcerative effect of Punica granatum extract and its ellagic acid rich fraction were comparable to sulphasalazine (100 mg/kg, p.o.) and sodium cromoglycate (40 mg/kg i.p). It is concluded that Punica granatum has a potential for ameliorating DSS-induced colitis and its ellagic acid rich fraction may be responsible for this effect. Further, the antiulcerative effects may be attributed to mast cell stabilizing, antiinflammatory and antioxidant actions.
Department of Pharmaceutical Sciences and Drug Research, Punjabi University, Patiala 147002, India. nirmal_puru@rediffmail.com
Kavinder
K
Singh
Amteshwar Singh
AS
Jaggi
Nirmal
N
Singh
D000818:Animals; D000897:Anti-Ulcer Agents / Q000627:therapeutic use*; D003093:Colitis, Ulcerative / Q000139:chemically induced / Q000188:drug therapy*; D003106:Colon / Q000187:drug effects / Q000473:pathology; D016264:Dextran Sulfate; D004610:Ellagic Acid / Q000627:therapeutic use*; D006632:Histamine / Q000378:metabolism; D008054:Lipid Peroxides / Q000378:metabolism; D029561:Lythraceae / Q000737:chemistry*; D051379:Mice; D018384:Oxidative Stress / Q000187:drug effects; D009195:Peroxidase / Q000378:metabolism; D008517:Phytotherapy; D010936:Plant Extracts / Q000627:therapeutic use*; D013481:Superoxides / Q000378:metabolism
D000897:Anti-Ulcer Agents; D008054:Lipid Peroxides; D010936:Plant Extracts; D013481:Superoxides; D004610:Ellagic Acid; D006632:Histamine; D016264:Dextran Sulfate; D009195:Peroxidase