Novel surface modified molecularly imprinted polymer using acryloyl-beta-cyclodextrin and acrylamide as monomers for selective recognition of lysozyme in aqueous solution.
A novel protein imprinted polymer for selective recognition of lysozyme was obtained. Acryloyl-beta-cyclodextrin, which offered hydrophilic exterior and hydrophobic cavity that were allowed to self-assemble with the template protein through hydrogen interaction and hydrophobic interaction, was synthesized and used as the functional monomer. Polymerization was carried out in the presence of acrylamide as an assistant monomer, which resulted in a new type of protein imprinted polymer. Langmuir adsorption model was employed to describe the isotherms, and maximum adsorption capacity was evaluated. The performance of such imprinted polymer was further demonstrated by high-performance liquid chromatography, and the results showed that the column packed with the lysozyme imprinted silica beads could effectively separate lysozyme from the mixture of lysozyme-cytochrome c, lysozyme-bovine serum albumin, lysozyme-avidin or lysozyme-methylated bovine serum albumin, which showed its high selectivity.
Zhang
Wei
W
Department of Chemistry, Nankai University, Tianjin, China.
Qin
Lei
L
He
Xi-Wen
XW
Li
Wen-You
WY
Zhang
Yu-Kui
YK
D020106:Acrylamide / Q000737:chemistry*; D000180:Acrylic Resins / Q000737:chemistry*; D000327:Adsorption; D000818:Animals; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D004602:Elements; D008855:Microscopy, Electron, Scanning; D008863:Microspheres; D054802:Molecular Imprinting / Q000379:methods*; D009113:Muramidase / Q000032:analysis*; D009584:Nitrogen / Q000737:chemistry; D011108:Polymers / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000737:chemistry; D012822:Silicon Dioxide / Q000737:chemistry; D012996:Solutions; D013499:Surface Properties; D013696:Temperature; D047392:beta-Cyclodextrins / Q000737:chemistry*
D000180:Acrylic Resins; D004602:Elements; D011108:Polymers; D012996:Solutions; D047392:beta-Cyclodextrins; D020106:Acrylamide; D012710:Serum Albumin, Bovine; D012822:Silicon Dioxide; D009113:Muramidase; C031215:betadex; D009584:Nitrogen