Direct electrochemistry of catalase at multiwalled carbon nanotubes-nafion in presence of needle shaped DDAB for H2O2 sensor.
The direct electrochemistry of catalase (CAT) at didodecyldimethylammonium bromide (DDAB) present on nafion dispersed multiwalled carbon nanotubes (MWCNTs-NF) modified glassy carbon electrode (GCE) has been reported. The presence of DDAB in MWCNTs-NF-CAT film enhances the surface coverage concentration of CAT (Fe(III/II)) to 48%. Similarly, in presence of DDAB, there is a 57% enhancement in electron transfer rate (ks) with 66% increase in CAT stability. (Fe(III/II)) redox couple exhibits linear dependence with the pH variation (-51 mV pH(-1)). The UV-vis absorption spectroscopy study reveals the entrapped CAT in DDAB film retains its native structure at MWCNTs-NF modified electrodes. Similarly, electrochemical impedance spectroscopy results confirm the co-existence of CAT and DDAB in the modified film. Further, scanning electron microscopy results reveal the structural morphological difference between various components in MWCNTs-NF-(DDAB/CAT) film. The cyclic voltammetry (CV) and amperometry (i-t curve) have been used for the measurement of electroanalytical properties of H2O2 by means of various film modified GCEs. The sensitivity values of MWCNTs-NF-(DDAB/CAT) film for H2O2 using CV (35.62 microA mM(-1)cm2) are higher than the values which are obtained for MWCNTs-NF-CAT film (2.74 mmicroA mM(-1)cm2). Similarly, the sensitivity values using i-t curve are 101.74 microA mM(-1)cm2 for MWCNTs-NF-(DDAB/CAT) and 74.69 microA mM(-1)cm2 for MWCNTs-NF-CAT film. Finally, the diffusion coefficient of H2O2 at MWCNTs-NF-(DDAB/CAT) film (3.4 x 10(-10) cm2 s(-1)) has been calculated using rotating disc electrode studies.
Department of Chemical Engineering and Biotechnology, National Taipei University of Technology, No. 1, Section 3, Chung-Hsiao East Road, Taipei 106, Taiwan, ROC.
Periasamy
P
Arun Prakash
Umasankar
U
Yogeswaran
Shen-Ming
SM
Chen
D002374:Catalase / Q000378:metabolism*; D004058:Diffusion; D055664:Electrochemical Techniques / Q000295:instrumentation / Q000379:methods / Q000592:standards; D004566:Electrodes / Q000592:standards; D005465:Fluorocarbon Polymers; D006861:Hydrogen Peroxide / Q000032:analysis*; D037742:Nanotubes, Carbon / Q000737:chemistry; D010084:Oxidation-Reduction; D000644:Quaternary Ammonium Compounds
D005465:Fluorocarbon Polymers; D037742:Nanotubes, Carbon; D000644:Quaternary Ammonium Compounds; C046112:didodecyldimethylammonium; C040402:perfluorosulfonic acid; D006861:Hydrogen Peroxide; D002374:Catalase