Comprehensive proteome analysis of lettuce latex using multidimensional protein-identification technology.
Commercially, lettuce (Lactuca sativa) is one of the most important leafy vegetables. Lettuce produces a milky latex of variable chemical compositions within its laticifers. As a step toward understanding the main physiological roles of this latex in higher plants, we embarked on its proteomic analysis. We investigated 587 latex proteins that were identified from the lettuce latex using multidimensional protein-identification technology. A bioinformatics analysis showed that the most frequently encountered proteins in the latex were organellar proteins from plastids and mitochondria, followed by nucleic and cytoplasmic proteins. Functional classification of the identified proteins showed that proteins related to metabolism, cell rescue, defense, and virulence were the most abundant in lettuce latex. Furthermore, numerous resistance proteins of lettuce and viral proteins were present in the latex suggesting for the first time a possible function of the lettuce latex in defense or pathogenesis. To the knowledge of the authors, this is the first large-scale proteome analysis of lettuce latex.
Cho
Won Kyong
WK
Division of Applied Life Science (BK21 program), Environmental Biotechnology, National Core Research Center, PMBBRC, Gyeongsang National University, Jinju 660-701, Republic of Korea.
Chen
Xiong-Yan
XY
Uddin
Nazim Mohamad
NM
Rim
Yeonggil
Y
Moon
Juyeon
J
Jung
Jin-Hee
JH
Shi
Chunlin
C
Chu
Hyosub
H
Kim
Suwha
S
Kim
Seon-Won
SW
Park
Zee-Yong
ZY
Kim
Jae-Yean
JY
D002853:Chromatography, Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D007840:Latex / Q000737:chemistry*; D018545:Lactuca / Q000737:chemistry*; D010940:Plant Proteins / Q000737:chemistry* / Q000502:physiology; D020543:Proteome; D013347:Subcellular Fractions / Q000378:metabolism; D053719:Tandem Mass Spectrometry
D007840:Latex; D010940:Plant Proteins; D020543:Proteome