Microfluidic cell counter with embedded optical fibers fabricated by femtosecond laser ablation and anodic bonding.
A simple fabrication technique to create all silicon/glass microfluidic devices is demonstrated using femtosecond laser ablation and anodic bonding. In a first application, we constructed a cell counting device based on small angle light scattering. The counter featured embedded optical fibers for multiangle excitation and detection of scattered light and/or fluorescence. The performance of the microfluidic cell counter was benchmarked against a commercial fluorescence-activated cell sorter.
Schafer
Dawn
D
Department of Physics, Colorado School of Mines, Golden, Colorado 80401, USA. dschafer@mines.edu
Gibson
Emily A
EA
Salim
Evan A
EA
Palmer
Amy E
AE
Jimenez
Ralph
R
Squier
Jeff
J
D002452:Cell Count / Q000295:instrumentation*; D002469:Cell Separation / Q000295:instrumentation*; D004566:Electrodes; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005336:Fiber Optic Technology / Q000295:instrumentation*; D007834:Lasers; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D012031:Refractometry / Q000295:instrumentation*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity