Localizing proteins by tissue printing.
The simple technique of making tissue prints on appropriate substrate material has made possible the easy localization of proteins, nucleic acids, carbohydrates, and small molecules in a tissue-specific mode. Plant tissues can be used to produce prints revealing a remarkable amount of anatomical detail, even without staining, which might be used to record developmental changes over time. In this chapter we will focus on the protocols for the localization of proteins and glycans using antibodies or lectins, probably the most frequently used application, but the localization of other molecules is reported and the sources indicated.
Pont-Lezica
Rafael F
RF
Surfaces Cellulaires et Signalisation chez les Végétaux, UMR 5546 CNRS-Université Paul Sabatier-Toulouse III, Pôle de Biotechnologie végétale, 24 Chemin de Borde Rouge BP, 42617 Auzeville, 31326, Castanet-Tolosan, France. lezica@scsv.ups-tlse.fr
D000818:Animals; D015151:Immunoblotting / Q000295:instrumentation / Q000379:methods*; D037121:Plant Lectins / Q000032:analysis*; D010940:Plant Proteins / Q000032:analysis*; D010944:Plants / Q000033:anatomy & histology / Q000737:chemistry
D037121:Plant Lectins; D010940:Plant Proteins