Three-dimensional gastric cancer cell culture using nanofiber scaffold for chemosensitivity test.
A three-dimensional (3D) culture of cancer cells has long been advocated as a better model of the malignant phenotype that is most closely related to tumorigenicity in vivo. To investigate the sensitivity of cancer cells to anticancer drugs, nanofiber scaffolds composed of PHBV and collagen peptide were fabricated by electrospinning. A 3D culture of cancer cells was successfully achieved by the use of nanofiber scaffolds. From the result of a chemosensitivity test, it was found that higher concentrations of anticancer drugs were required to achieve a comparable cytotoxic effect in 3D culture due to their structural architecture. These data demonstrate that the electrospun nanofiber scaffolds can provide a 3D model particularly appropriate for investigating mechanisms involved in cancer cell sensitivity to anticancer drugs.
Kim
Young-Jin
YJ
Department of Biomedical Engineering, Catholic University of Daegu, 330 Geumnak 1-ri, Hayang-eup, Gyeongsan, South Korea. yjkim@cu.ac.kr
Bae
Han-Ik
HI
Kwon
Oh Kyoung
OK
Choi
Myung-Seok
MS
D000970:Antineoplastic Agents / Q000494:pharmacology*; D018929:Cell Culture Techniques / Q000379:methods*; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D006801:Humans; D020128:Inhibitory Concentration 50; D008855:Microscopy, Electron, Scanning; D049329:Nanostructures / Q000627:therapeutic use* / Q000648:ultrastructure; D011091:Polyesters; D013274:Stomach Neoplasms; D013778:Tetrazolium Salts; D013844:Thiazoles; D000094122:Polyhydroxybutyrates
D000970:Antineoplastic Agents; D011091:Polyesters; D013778:Tetrazolium Salts; D013844:Thiazoles; C052620:poly(3-hydroxybutyrate)-co-(3-hydroxyvalerate); D000094122:Polyhydroxybutyrates; C022616:thiazolyl blue