Cerebrospinal fluid from sporadic amyotrophic lateral sclerosis patients induces degeneration of a cultured motor neuron cell line.
We investigated the effect of Cerebrospinal Fluid (CSF) from sporadic Amyotrophic Lateral Sclerosis patients (SALS-CSF) on motor neuron-like cells to delineate the pathomechanism of SALS. Exposure of NSC-34 cells to SALS-CSF caused lower viability, reduction in differentiation and enhanced lactate dehydrogenase activity. Additionally, reduced choline acetyl transferase expression alongside intracellular aggregation of phosphorylated neurofilaments was prominently seen. The aggregates were immunopositive for ubiquitin. These findings are comparable to the pathological changes seen in the postmortem tissue of ALS patients. Unlimited supply of NSC-34 cells and their vulnerability to SALS-CSF render them to be a good bioassay system to identify new therapeutic agents conferring protection to motor neurons.
Department of Neurophysiology, National Institute of Mental Health and Neuro Sciences, Bangalore, India.
K
K
Vijayalakshmi
Phalguni Anand
PA
Alladi
T N
TN
Sathyaprabha
Jamuna R
JR
Subramaniam
A
A
Nalini
T R
TR
Raju
D000690:Amyotrophic Lateral Sclerosis / Q000134:cerebrospinal fluid*; D000704:Analysis of Variance; D000818:Animals; D015153:Blotting, Western; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000502:physiology; D002795:Choline O-Acetyltransferase / Q000378:metabolism; D003599:Cytoskeleton / Q000378:metabolism; D015870:Gene Expression; D006019:Glycolysis / Q000502:physiology; D006801:Humans; D051379:Mice; D018613:Microscopy, Confocal; D009046:Motor Neurons / Q000473:pathology / Q000502:physiology*; D009410:Nerve Degeneration / Q000503:physiopathology*; D055495:Neurogenesis / Q000502:physiology; D010766:Phosphorylation; D010784:Photomicrography; D025801:Ubiquitin / Q000378:metabolism
D025801:Ubiquitin; D002795:Choline O-Acetyltransferase