Development and validation of a gold nanoparticle immunochromatographic assay (ICG) for the detection of zearalenone.
A monoclonal antibody (mAb)-based gold nanoparticle immunochromatographic assay (ICG) for zearalenone detection was developed, optimized, and validated. The detection limits of ICG optimized with appropriate amounts of zearalenone-bovine serum albumin and gold nanoparticle-mAb to zearalenone were 2.5 ng/mL and 30 μg/kg for the standard solution and spike sample, respectively, and a weak cross-reaction for α-zearalenol and β-zearalenol was observed. The assay required only 15 min to obtain results and one step to perform the assay. In validation, the results obtained from spiked corn (10, 20, 30, 50, and 100 μg/kg) and naturally contaminated corn samples by the ICG were in good agreement with those obtained by direct competitive enzyme-linked immunosorbent assay (DC-ELISA) and high-performance liquid chromatography (HPLC). Therefore, the results obtained in this study could be used as basic research for the development of zearalenone-ICG, and the ICG developed could be a useful on-site screening tool for the rapid detection of zearalenone in corn without special instrumentation.
Shim
Won-Bo
WB
Division of Applied Life Science (BK 21 program), Graduate School, Institute of Agriculture and Life Science, Gyeongsang National University, Chinju, Gyeongnam 660-701, Korea.
Kim
Kyeong-Yeol
KY
Chung
Duck-Hwa
DH
D000911:Antibodies, Monoclonal; D002845:Chromatography / Q000379:methods*; D004968:Estrogens, Non-Steroidal / Q000032:analysis*; D005506:Food Contamination / Q000032:analysis; D006046:Gold; D007118:Immunoassay / Q000379:methods*; D053758:Nanoparticles; D015203:Reproducibility of Results; D003313:Zea mays / Q000737:chemistry; D015025:Zearalenone / Q000032:analysis*
D000911:Antibodies, Monoclonal; D004968:Estrogens, Non-Steroidal; D015025:Zearalenone; D006046:Gold