Endothelial nitric oxide synthetase, methylenetetrahydrofolate reductase polymorphisms, and cardiovascular complications in Tunisian patients with nondiabetic renal disease.
OBJECTIVE
The relationship between endothelial nitric oxide synthase (eNOS), methylenetetrahydrofolate reductase (MTHFR) gene polymorphisms and cardiovascular disease (CVD) in Tunisian patients with chronic renal disease (CRD) has not been examined. We investigated (a) the relationship of these gene polymorphisms with the presence and the severity of renal disease, and (b) their relationships with CVD in these patients.


METHODS
We used PCR-RFLP analysis to detect the eNOS G894T, MTHFR C677T and A1298C variants in 100 patients with CRD and in 120 healthy controls.


RESULTS
MTHFR C677T and A1298C polymorphisms were not associated with the presence of renal disease. However, we found that eNOS G894T polymorphism was associated with the presence and severity of renal disease and with CVD in CRD patients (P=0.028, P=0.018, P=0.016 respectively). We showed that 894T allele was an independent risk factor of severity of renal disease and the incidence of CVD (P=0.01 and P<0.01 respectively).


CONCLUSIONS
The G894T polymorphism of the eNOS gene is associated with severity of renal disease. Presence of the 894T allele aggravated renal damage and increased the incidence of CVD in Tunisian CRD patients.
Research Unit 03/UR/08-14, Faculty of Pharmacy, 5000-Monastir, Tunisia. Mohsen.kerkeni@yahoo.fr
Mohsen
M
Kerkeni
Ahmed
A
Letaief
Abdellatif
A
Achour
Abdelhedi
A
Miled
Francois
F
Trivin
Khira
K
Maaroufi
D044383:Black People / Q000235:genetics; D002318:Cardiovascular Diseases / Q000150:complications* / Q000201:enzymology* / Q000235:genetics; D016022:Case-Control Studies; D003920:Diabetes Mellitus / Q000473:pathology; D005260:Female; D005787:Gene Frequency; D005838:Genotype; D006801:Humans; D007674:Kidney Diseases / Q000150:complications* / Q000201:enzymology* / Q000235:genetics; D007676:Kidney Failure, Chronic / Q000150:complications / Q000201:enzymology / Q000235:genetics; D008297:Male; D042965:Methylenetetrahydrofolate Reductase (NADPH2) / Q000235:genetics*; D008875:Middle Aged; D015999:Multivariate Analysis; D052250:Nitric Oxide Synthase Type III / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D014416:Tunisia
C496314:NOS3 protein, human; D052250:Nitric Oxide Synthase Type III; D042965:Methylenetetrahydrofolate Reductase (NADPH2)