Locked nucleic acid based beacons for surface interaction studies and biosensor development.
DNA sensors and microarrays permit fast, simple, and real-time detection of nucleic acids through the design and use of increasingly sensitive, selective, and robust molecular probes. Specifically, molecular beacons (MBs) have been employed for this purpose; however, their potential in the development of solid-surface-based biosensors has not been fully realized. This is mainly a consequence of the beacon's poor stability because of the hairpin structure once immobilized onto a solid surface, commonly resulting in a low signal enhancement. Here, we report the design of a new MB that overcomes some of the limitations of MBs for surface immobilization. Essentially, this new design adds locked nucleic acid bases (LNAs) to the beacon structure, resulting in a LNA molecular beacon (LMB) with robust stability after surface immobilization. To test the efficacy of LMBs against that of regular molecular beacons (RMBs), the properties of selectivity, sensitivity, thermal stability, hybridization kinetics, and robustness for the detection of target sequences were compared and evaluated. A 25-fold enhancement was achieved for the LMB on surface with detection limits reaching the low nanomolar range. In addition, the LMB-based biosensor was shown to possess better stability, reproducibility, selectivity, and robustness when compared to the RMB. Therefore, as an alternative to conventional DNA and as a prospective tool for use in both DNA microarrays and biosensors, these results demonstrate the potential of the locked nucleic acid bases for nucleic acid design for surface immobilization.
Center for Research at the Bio/Nano Interface, Department of Chemistry, University of Florida, Gainesville, Florida 32611, USA.
Karen
K
Martinez
M-Carmen
MC
Estevez
Yanrong
Y
Wu
Joseph A
JA
Phillips
Colin D
CD
Medley
Weihong
W
Tan
D000818:Animals; D001483:Base Sequence; D015374:Biosensing Techniques / Q000379:methods*; D001709:Biotechnology; D002417:Cattle; D002457:Cell Extracts; D007700:Kinetics; D009693:Nucleic Acid Hybridization; D015341:Nucleic Acid Probes / Q000138:chemical synthesis* / Q000737:chemistry / Q000235:genetics; D020411:Oligonucleotide Array Sequence Analysis; D009841:Oligonucleotides / Q000737:chemistry*; D012680:Sensitivity and Specificity; D013499:Surface Properties; D013696:Temperature
D002457:Cell Extracts; D015341:Nucleic Acid Probes; D009841:Oligonucleotides; C477371:locked nucleic acid