Assessment of zona pellucida glycoprotein and integrin transcript contents in porcine oocytes.
Using reverse transcription and real-time quantitative PCR analysis we evaluated the transcript levels of integrins (alphaL, alphaM, beta1, and beta6), CD9 and CD18 antigens as well as zona pellucida glycoproteins (pZP1, pZP2, pZP3 and pZP3alpha) in oocytes isolated from puberal gilts (n=20) and multiparous sows (n=20). We found significantly (p<0.05) higher transcript contents of alphaL, alphaM, beta1, and beta integrins, CD9 antigen, and pZP2 and pZP3 in puberal gilt oocytes compared to multiparous sow oocytes. Our results suggest that a decrease in the level of oocyte transcripts encoding essential proteins involved in oocyte fertilization may be associated with increased porcine female age.
Kempisty
Bartosz
B
Department of Biochemistry, University of Medical Sciences, 6 Swiecickiego St., 60-781 Poznań, Poland.
Antosik
Paweł
P
Bukowska
Dorota
D
Jackowska
Marta
M
Lianeri
Margarita
M
Jaśkowski
Jedrzej M
JM
Jagodziński
Paweł P
PP
D000375:Aging; D000818:Animals; D015703:Antigens, CD / Q000032:analysis; D018821:CD18 Antigens / Q000032:analysis; D004527:Egg Proteins / Q000032:analysis; D005260:Female; D005306:Fertilization; D039001:Integrin alpha Chains / Q000235:genetics; D039641:Integrin beta Chains / Q000235:genetics; D016023:Integrins / Q000235:genetics*; D008562:Membrane Glycoproteins / Q000032:analysis*; D009865:Oocytes / Q000737:chemistry*; D012333:RNA, Messenger / Q000032:analysis*; D011956:Receptors, Cell Surface; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013552:Swine; D060245:Tetraspanin 29; D015044:Zona Pellucida / Q000737:chemistry*; D000072216:Zona Pellucida Glycoproteins
D015703:Antigens, CD; D018821:CD18 Antigens; D004527:Egg Proteins; D039001:Integrin alpha Chains; D039641:Integrin beta Chains; D016023:Integrins; D008562:Membrane Glycoproteins; D012333:RNA, Messenger; D011956:Receptors, Cell Surface; D060245:Tetraspanin 29; D000072216:Zona Pellucida Glycoproteins