Generation of retroviral macrophage cDNA expression libraries and functional screening for surface receptors.
Macrophages, tissue-based phagocytic cells derived from blood monocytes, play important roles in immunity and homeostasis. As professional scavengers, macrophages phagocytose microbes, apoptotic and necrotic cells and take up modified lipoprotein particles. However, many of their complex interactions with other immune cells and/or various ligands are not yet clearly understood. To identify and isolate macrophage cell surface molecules, particularly macrophage receptors, for which ligands are known, a powerful generalized screening method has been established. As discussed in this chapter, this technique based on function has been successfully applied for the identification of dectin-1, the major macrophage receptor involved in the binding and recognition of beta-glucans (Nature 413:36-37, 2001).
Institute for Infectious Diseases and Molecular Medicine, Faculty of Health Sciences, University of Cape Town, Cape Town, South Africa.
Georgia
G
Schäfer
Gordon D
GD
Brown
D000818:Animals; D016650:Fluorescein-5-isothiocyanate / Q000378:metabolism; D015723:Gene Library; D008264:Macrophages / Q000378:metabolism*; D051379:Mice; D008967:Molecular Biology / Q000379:methods*; D041681:NIH 3T3 Cells; D011956:Receptors, Cell Surface / Q000032:analysis* / Q000378:metabolism; D012190:Retroviridae / Q000235:genetics*
D011956:Receptors, Cell Surface; D016650:Fluorescein-5-isothiocyanate