Deferoxamine reduces intracerebral hematoma-induced iron accumulation and neuronal death in piglets.
OBJECTIVE
Our previous studies found that deferoxamine reduces intracerebral hemorrhage (ICH)-induced brain injury in rats. The current study examined whether deferoxamine reduces brain injury in a piglet ICH model.


METHODS
Pigs received an injection of autologous blood into the right frontal lobe. Deferoxamine (50 mg/kg, IM) or vehicle was administered 2 hours after ICH and then every 12 hours up to 7 days. Animals were killed 3 or 7 days later to examine iron accumulation, white matter injury, and neuronal death.


RESULTS
ICH resulted in development of a reddish perihematomal zone, and iron accumulation, ferritin upregulation, and neuronal death within that zone. Deferoxamine reduced the perihematomal reddish zone, white matter injury, and the number of Perls', ferritin, and Fluoro-Jade C-positive cells.


CONCLUSIONS
Iron accumulation occurs in the piglet brain after ICH. Deferoxamine reduces ICH-induced iron buildup and brain injury in piglets.
Yuxiang
Y
Gu
Ya
Y
Hua
Richard F
RF
Keep
Lewis B
LB
Morgenstern
Guohua
G
Xi
D000818:Animals; D000931:Antidotes / Q000627:therapeutic use*; D001921:Brain / Q000473:pathology; D016923:Cell Death; D020202:Cerebral Hemorrhage, Traumatic / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D003676:Deferoxamine / Q000627:therapeutic use*; D005293:Ferritins / Q000378:metabolism; D005452:Fluoresceins; D005456:Fluorescent Dyes; D007501:Iron / Q000378:metabolism*; D009474:Neurons / Q000473:pathology*; D009930:Organic Chemicals; D013552:Swine
D000931:Antidotes; D005452:Fluoresceins; D005456:Fluorescent Dyes; D009930:Organic Chemicals; C435731:fluoro jade; D005293:Ferritins; D007501:Iron; D003676:Deferoxamine