Ingestion of bacteria overproducing DnaK attenuates Vibrio infection of Artemia franciscana larvae.
Feeding of bacterially encapsulated heat shock proteins (Hsps) to invertebrates is a novel way to limit Vibrio infection. As an example, ingestion of Escherichia coli overproducing prokaryotic Hsps significantly improves survival of gnotobiotically cultured Artemia larvae upon challenge with pathogenic Vibrio campbellii. The relationship between Hsp accumulation and enhanced resistance to infection may involve DnaK, the prokaryotic equivalent to Hsp70, a major molecular chaperone in eukaryotic cells. In support of this proposal, heat-stressed bacterial strains LVS 2 (Bacillus sp.), LVS 3 (Aeromonas hydrophila), LVS 8 (Vibrio sp.), GR 8 (Cytophaga sp.), and GR 10 (Roseobacter sp.) were shown in this work to be more effective than nonheated bacteria in protecting gnotobiotic Artemia larvae against V. campbellii challenge. Immunoprobing of Western blots and quantification by enzyme-linked immunosorbent assay revealed that the amount of DnaK in bacteria and their ability to enhance larval resistance to infection by V. campbellii are correlated. Although the function of DnaK is uncertain, it may improve tolerance to V. campbellii via immune stimulation, a possibility of significance from a fundamental perspective and also because it could be applied in aquaculture, a major method of food production.
Sung
Yeong Yik
YY
Department of Fisheries and Aquaculture, Faculty of Agrotechnology and Food Science, University Malaysia Terengganu, 21030 Kuala Terengganu, Malaysia. yeong@umt.edu.my
Dhaene
Till
T
Defoirdt
Tom
T
Boon
Nico
N
MacRae
Thomas H
TH
Sorgeloos
Patrick
P
Bossier
Peter
P
D000251:Adenosine Triphosphatases / Q000096:biosynthesis* / Q000276:immunology; D000821:Animal Feed; D000818:Animals; D001156:Artemia / Q000382:microbiology* / Q000502:physiology; D001426:Bacterial Proteins / Q000096:biosynthesis* / Q000276:immunology; D006090:Gram-Negative Bacteria / Q000378:metabolism; D018840:HSP70 Heat-Shock Proteins / Q000096:biosynthesis / Q000276:immunology; D018869:Heat-Shock Response / Q000502:physiology; D007113:Immunity, Innate; D007814:Larva / Q000382:microbiology / Q000502:physiology; D018832:Molecular Chaperones / Q000096:biosynthesis* / Q000276:immunology; D015854:Up-Regulation; D014735:Vibrio Infections / Q000276:immunology* / Q000378:metabolism
D001426:Bacterial Proteins; D018840:HSP70 Heat-Shock Proteins; D018832:Molecular Chaperones; D000251:Adenosine Triphosphatases; C115251:DnaK protein, Brevibacillus choshinensis