Kinematic viscosity of therapeutic pulmonary surfactants with added polymers.
The addition of various polymers to pulmonary surfactants improves surface activity in experiments both in vitro and in vivo. Although the viscosity of surfactants has been investigated, the viscosity of surfactant polymer mixtures has not. In this study, we have measured the viscosities of Survanta and Infasurf with and without the addition of polyethylene glycol, dextran or hyaluronan. The measurements were carried out over a range of surfactant concentrations using two concentrations of polymers at two temperatures. Our results indicate that at lower surfactant concentrations, the addition of any polymers increased the viscosity. However, the addition of polyethylene glycol and dextran to surfactants at clinically used concentrations can substantially lower viscosity. Addition of hyaluronan at clinical surfactant concentrations slightly increased Infasurf viscosity and produced little change in Survanta viscosity. Effects of polymers on viscosity correlate with changes in size and distribution of surfactant aggregates and the apparent free volume of liquid as estimated by light microscopy. Aggregation of surfactant vesicles caused by polymers may therefore not only improve surface activity as previously shown, but may also affect viscosity in ways that could improve surfactant distribution in vivo.
Department of Pediatrics, University of California, San Francisco, USA. klu@sfghpeds.ucsf.edu
Karen W
KW
Lu
Jesús
J
Pérez-Gil
H  William
H
Taeusch
D001688:Biological Products / Q000737:chemistry*; D003911:Dextrans / Q000494:pharmacology*; D006820:Hyaluronic Acid / Q000494:pharmacology*; D008854:Microscopy, Electron; D011092:Polyethylene Glycols / Q000494:pharmacology*; D011663:Pulmonary Surfactants / Q000737:chemistry* / Q000494:pharmacology; D014783:Viscosity
D001688:Biological Products; D003911:Dextrans; D011663:Pulmonary Surfactants; D011092:Polyethylene Glycols; D006820:Hyaluronic Acid; C117342:calfactant; C072197:beractant