Distinct spatiotemporal pattern of CNS lesions revealed by USPIO-enhanced MRI in MOG-induced EAE rats implicates the involvement of spino-olivocerebellar pathways.
USPIO-enhanced MRI allows non-invasive visualization of mononuclear cell infiltration into CNS lesions in MS and EAE. Herein, we show a distinct spatiotemporal pattern of CNS lesions that reveals the involvement of spino-olivocerebellar pathways in MOG-induced EAE rats using USPIO-enhanced MRI. Specifically, lesions of the inferior olives were observed primarily in the acute phase whereas lesions of cerebellum or spinal cord/brainstem were observed during the relapse phase. Further, behavioral deficits observed from these animals are consistent with the functional role of spino-olivocerebellar pathways in coordination and movement. Collectively, our results provide new insights into the pathophysiology of this animal model of MS.
Advanced Technology, Global Pharmaceutical Research and Development, Abbott Laboratories, Abbott Park, IL 60064, USA. chih-liang.chin@abbott.com
Chih-Liang
CL
Chin
Madhavi
M
Pai
Peter F
PF
Bousquet
Annette J
AJ
Schwartz
Elizabeth M
EM
O'Connor
Christine M
CM
Nelson
Vincent P
VP
Hradil
Bryan F
BF
Cox
Bradford L
BL
McRae
Gerard B
GB
Fox
D000818:Animals; D001921:Brain / Q000473:pathology*; D002531:Cerebellum / Q000473:pathology; D003911:Dextrans; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000473:pathology*; D005260:Female; D052203:Ferrosoferric Oxide; D007150:Immunohistochemistry; D007501:Iron; D008279:Magnetic Resonance Imaging / Q000379:methods; D058185:Magnetite Nanoparticles; D009043:Motor Activity / Q000502:physiology; D009185:Myelin Proteins; D018992:Myelin-Associated Glycoprotein / Q000276:immunology / Q000633:toxicity; D063308:Myelin-Oligodendrocyte Glycoprotein; D009434:Neural Pathways / Q000473:pathology*; D009847:Olivary Nucleus / Q000473:pathology; D010087:Oxides; D019275:Radiopharmaceuticals; D051381:Rats; D013116:Spinal Cord / Q000473:pathology*
D003911:Dextrans; D058185:Magnetite Nanoparticles; C571328:Mog protein, rat; D009185:Myelin Proteins; D018992:Myelin-Associated Glycoprotein; D063308:Myelin-Oligodendrocyte Glycoprotein; D010087:Oxides; D019275:Radiopharmaceuticals; C097921:ferumoxtran-10; D007501:Iron; D052203:Ferrosoferric Oxide