Generation of red fluorescent protein transgenic dogs.
Dogs (Canis familiaris) share many common genetic diseases with humans and development of disease models using a transgenic approach has long been awaited. However, due to the technical difficulty in obtaining fertilizable eggs and the unavailability of embryonic stem cells, no transgenic dog has been generated. Canine fetal fibroblasts were stably transfected with a red fluorescent protein (RFP) gene-expressing construct using retrovirus gene delivery method. Somatic cell nuclear transfer was then employed to replace the nucleus of an oocyte with the nucleus of the RFP-fibroblasts. Using this approach, we produced the first generation of transgenic dogs with four female and two male expressing RFP.
Hong
So Gun
SG
Seoul National University, Korea.
Kim
Min Kyu
MK
Jang
Goo
G
Oh
Hyun Ju
HJ
Park
Jung Eun
JE
Kang
Jung Taek
JT
Koo
Ok Jae
OJ
Kim
Teoan
T
Kwon
Mo Sun
MS
Koo
Bon Chul
BC
Ra
Jeong Chan
JC
Kim
Dae Yong
DY
Ko
CheMyong
C
Lee
Byeong Chun
BC
D000818:Animals; D030801:Animals, Genetically Modified; D000831:Animals, Newborn; D015139:Blotting, Southern; D004285:Dogs / Q000235:genetics*; D004624:Embryo Transfer; D004622:Embryo, Mammalian / Q000378:metabolism; D005260:Female; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism; D005822:Genetic Vectors / Q000235:genetics; D005838:Genotype; D017404:In Situ Hybridization, Fluorescence; D007621:Karyotyping; D008164:Luminescent Proteins / Q000235:genetics* / Q000378:metabolism; D008297:Male; D008856:Microscopy, Fluorescence; D053652:Nuclear Transfer Techniques; D009865:Oocytes / Q000166:cytology / Q000378:metabolism; D011247:Pregnancy; D012190:Retroviridae / Q000235:genetics; D014162:Transfection; D019076:Transgenes / Q000235:genetics*; D000097573:Red Fluorescent Protein
D008164:Luminescent Proteins; D000097573:Red Fluorescent Protein