Effect of Bu-Zhong-Yi-Qi-Tang on deficiency of N-glycan/nitric oxide and islet damage induced by streptozotocin in diabetic rats.
OBJECTIVE
To investigate the effect of Bu-Zhong-Yi-Qi-Tang (Decoction for Reinforcing Middle Jiao and Replenishing Qi) on deficiency of N-glycan/nitric oxide (NO) and islet damage induced by injecting two medium doses of streptozotocin (STZ).


METHODS
Diabetes was induced by intraperitoneal injection of STZ at 55 mg/kg on day 1 and day 8. Islet damage was evaluated using a scoring system. Nitrite, nitrate, alpha-mannosidase and amylase activities were measured by colorimetry. N-glycan patterns of amylase were determined with lectin [ConA, pisum sativum agglutinin (PSA), peanut agglutinin (PNA), and lens culinaris agglutinin (LCA)] affinity precipitation method.


RESULTS
Severe islet necrosis and mild islet atrophy were observed in diabetic rats. The number and size of islets, the activities of alpha-mannosidase, amylase and nitrite were decreased, while the binding of PNA and LCA to amylase was increased. All of which were improved after treatment with Bu-Zhong-Yi-Qi-Tang. Islet damage was significantly correlated with nitrite, nitrate, alpha-mannosidase, amylase and the binding of LCA, PNA, and PSA to amylase.


CONCLUSIONS
STZ-induced islet damage is related to N-glycan deficiency in proteins by blocking alpha-mannosidase activity and no deficiency, accumulation of unfolded proteins, and endoplasmic reticulum stress and activation of cellular signals, all of which are improved after treatment with Bu-Zhong-Yi-Qi-Tang.
Pi-Wei Institute, Guangzhou University of Traditional Chinese Medicine, No. 12 Jichang Road, Guangzhou, Guangdong Province, China. lxq6298@yahoo.com.cn
Xiao-Qiu
XQ
Liu
Ling
L
Wu
Xue-Jun
XJ
Guo
D000681:Amylases / Q000378:metabolism; D000818:Animals; D003921:Diabetes Mellitus, Experimental / Q000188:drug therapy / Q000378:metabolism; D004365:Drugs, Chinese Herbal / Q000627:therapeutic use*; D006801:Humans; D007515:Islets of Langerhans / Q000166:cytology / Q000378:metabolism / Q000473:pathology; D037102:Lectins / Q000378:metabolism; D009566:Nitrates / Q000378:metabolism; D009569:Nitric Oxide / Q000378:metabolism*; D009573:Nitrites / Q000378:metabolism; D011134:Polysaccharides / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D043323:alpha-Mannosidase / Q000378:metabolism
D004365:Drugs, Chinese Herbal; D037102:Lectins; D009566:Nitrates; D009573:Nitrites; D011134:Polysaccharides; C402943:bu-zhong-yi-qi-tang; D009569:Nitric Oxide; D000681:Amylases; D043323:alpha-Mannosidase