Role of purines on hepatic ischemia-reperfusion lesions in rabbit.
In this work, we evaluate the effects of adenosine 5' triphosphate (ATP) on hepatic lesions caused by ischemia/reperfusion (I/R) in liver rabbit. Rabbits were pretreated with ATP (15 mg/kg IV) or saline solution 0.9% (SS), before the hepatic I/R procedure. We evaluated the effects of ATP on hepatic injury before and after I/R. The warm hepatic I/R procedure caused profound acute liver injury, as indicated by elevated serum aspartate aminotransferase, alanine aminotransferase, and lactic dehydrogenase levels, as well as a high apoptotic cell count. All these changes were attenuate by ATP treatment before the hepatic I/R procedure. These results suggested that ATP exerted protective effects on hepatic I/R lesions in the rabbit. This ATP effect may be related to improved energy metabolism during reperfusion in ischemic livers protecting against functional damage of cellular and subcellular membranes during lipid peroxidation.
Taha
M O
MO
Department of Surgery, Federal University of São Paulo, Escola Paulista de Medicina, São Paulo, Brazil. taha@uol.com.br
Simões
M J
MJ
Mendonça
M V
MV
Lugo
F R C
FR
Carmo-Filho
R
R
Lazarini
J L
JL
Bessa
M Z
MZ
Marconi
T D
TD
Magalhães
K G
KG
Soares
J H
JH
Tersariol
I L S
IL
Monteiro
H P
HP
Oliveira-Júnior
I S
IS
Oliveira
I
I
Jurkiewicz
A
A
Caricati-Neto
A
A
D000255:Adenosine Triphosphate / Q000627:therapeutic use; D000410:Alanine Transaminase / Q000187:drug effects / Q000378:metabolism; D000818:Animals; D001219:Aspartate Aminotransferases / Q000187:drug effects / Q000378:metabolism; D007511:Ischemia / Q000503:physiopathology; D007770:L-Lactate Dehydrogenase / Q000187:drug effects / Q000378:metabolism; D008099:Liver / Q000187:drug effects / Q000503:physiopathology; D008107:Liver Diseases / Q000503:physiopathology* / Q000517:prevention & control; D008297:Male; D008930:Mitochondria, Liver / Q000187:drug effects / Q000378:metabolism; D011687:Purines / Q000378:metabolism*; D011817:Rabbits; D015427:Reperfusion Injury / Q000503:physiopathology* / Q000517:prevention & control
D011687:Purines; D000255:Adenosine Triphosphate; D007770:L-Lactate Dehydrogenase; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase