Surface plasmon resonance immunoassay analysis of pituitary hormones in urine and serum samples.
BACKGROUND
Direct determination of four pituitary peptide hormones: human thyroid stimulating hormone (hTSH), growth hormone (hGH), follicle stimulating hormone (hFSH), and luteinizing hormone (hLH) has been carried out using a portable surface plasmon resonance (SPR) immunosensor.


METHODS
A commercial SPR biosensor was employed. The immobilization of the hormones was optimized and monoclonal antibodies were selected in order to obtain the best sensor performance. Assay parameters as running buffer and regeneration solution composition or antibody concentration were adjusted to achieve a sensitive analyte detection.


RESULTS
The performance of the assays was assessed in buffer solution, serum and urine, showing sensitivity in the range from 1 to 6 ng/mL. The covalent attachment of the hormones ensured the stability of the SPR signal through repeated use in up to 100 consecutive assay cycles. Mean intra- and inter-day coefficients of variation were all <7%, while batch-assay variability using different sensor surfaces was <5%.


CONCLUSIONS
Taking account both the excellent reutilization performance and the outstanding reproducibility, this SPR immunoassay method turns on a highly reliable tool for endocrine monitoring in laboratory and point-of-care (POC) settings.
Grupo de Nanobiosensores y Biofísica Molecular, Centro de Investigación en Nanociencia y Nanotecnología (CSIC-ICN), ETSE, Campus UAB, Bellaterra, Barcelona, Spain. juan.trevino@cin2.es
Juan
J
Treviño
Ana
A
Calle
José Miguel
JM
Rodríguez-Frade
Mario
M
Mellado
Laura M
LM
Lechuga
D053000:Analytic Sample Preparation Methods; D005640:Follicle Stimulating Hormone / Q000097:blood / Q000652:urine; D013006:Growth Hormone / Q000097:blood / Q000652:urine; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D007753:Laboratories; D007986:Luteinizing Hormone / Q000097:blood / Q000652:urine; D010907:Pituitary Hormones / Q000097:blood* / Q000652:urine*; D019095:Point-of-Care Systems; D012680:Sensitivity and Specificity; D013048:Specimen Handling; D020349:Surface Plasmon Resonance; D013997:Time Factors
D010907:Pituitary Hormones; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone; D013006:Growth Hormone