High-dimensional data analysis: selection of variables, data compression and graphics--application to gene expression.
The paper presents effective and mathematically exact procedures for selection of variables which are applicable in cases with a very high dimension as, for example, in gene expression analysis. Choosing sets of variables is an important method to increase the power of the statistical conclusions and to facilitate the biological interpretation. For the construction of sets, each single variable is considered as the centre of potential sets of variables. Testing for significance is carried out by means of the Westfall-Young principle based on resampling or by the parametric method of spherical tests. The particular requirements for statistical stability are taken into account; each kind of overfitting is avoided. Thus, high power is attained and the familywise type I error can be kept in spite of the large dimension. To obtain graphical representations by heat maps and curves, a specific data compression technique is applied. Gene expression data from B-cell lymphoma patients serve for the demonstration of the procedures.
Interdisciplinary Centre for Bioinformatics (IZBI), University of Leipzig, Härtelstr. 16-18, 04107 Leipzig, Germany. juergen.laeuter@med.ovgu.de
Jürgen
J
Läuter
Friedemann
F
Horn
Maciej
M
Rosołowski
Ekkehard
E
Glimm
D000465:Algorithms; D020869:Gene Expression Profiling / Q000379:methods; D006801:Humans; D016393:Lymphoma, B-Cell / Q000378:metabolism; D015233:Models, Statistical; D015999:Multivariate Analysis; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D012984:Software