Target-based drug discovery: the emerging success of frontal affinity chromatography coupled to mass spectrometry.
Frontal affinity chromatography coupled to mass spectrometry (FAC-MS) has been reported as a potential method for screening compound mixtures against immobilized target proteins. The potentiality of this analytical approach is described and illustrated with a number of examples based on targets of pharmaceutical interest.
Dipartimento di Chimica Farmaceutica, Università degli Studi di Pavia, Viale Taramelli 12, 27100 Pavia, Italy. enrica.calleri@unipv.it
Enrica
E
Calleri
Caterina
C
Temporini
Gabriele
G
Caccialanza
Gabriella
G
Massolini
D002846:Chromatography, Affinity / Q000379:methods*; D055808:Drug Discovery / Q000379:methods*; D055767:Immobilized Proteins / Q000737:chemistry / Q000378:metabolism*; D013058:Mass Spectrometry / Q000379:methods*; D054852:Small Molecule Libraries
D055767:Immobilized Proteins; D054852:Small Molecule Libraries