Epitope mapping by surface plasmon resonance.
Biacore systems (Biacore AB) enable label-free detection of molecular interactions in real time using surface plasmon resonance detection. Epitope mapping of antibodies is usually performed in a pairwise fashion where one antibody is used to capture the antigen from solution and the binding of a secondary antibody is monitored. In contrast to alternative approaches, the method allows for mapping of large matrices of antibody pairs without the need for cumbersome labeling steps, and the real-time analysis enables a better ranking of complex stability when compared with end-point assays.
Department of Systems and Applications, Biacore AB (a GE Healthcare Company), Rapsgatan 7, 754 50 Uppsala, Sweden.
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D000818:Animals; D000906:Antibodies / Q000032:analysis* / Q000737:chemistry / Q000276:immunology*; D055910:Antibodies, Immobilized / Q000737:chemistry / Q000276:immunology; D001666:Binding Sites, Antibody; D018604:Epitope Mapping / Q000191:economics / Q000379:methods*; D006801:Humans; D020349:Surface Plasmon Resonance / Q000379:methods*; D013997:Time Factors
D000906:Antibodies; D055910:Antibodies, Immobilized