l-Arginine supplementation protects against hepatic ischemia-reperfusion lesions in rabbits.
We evaluated the effects of a substrate in the biosynthesis of nitric oxide (NO)-l-arginine (LARG)-on hepatic lesions caused by ischemia/reperfusion (I/R) injury in rabbit livers. Rabbits were pretreated with LARG (150 mg/kg IV) or saline solution 0.9% (SS) before the hepatic I/R procedure. The effects of LARG on hepatic injury were evaluated before and after I/R. The warm hepatic I/R procedure produced profound acute liver injury, as indicated by elevated values of serum aspartate aminotransferase (AST), alanine aminotransferase (ALT), and lactic dehydrogenase (LDH), as well as a high apoptotic cell count. All changes were attenuated by treatment with LARG before the hepatic I/R procedure. These results suggested that LARG produced protective effects on hepatic I/R lesions. This protective effect of LARG was probably associated with blocking generation of superoxide anions during the hepatic I/R procedure.
Taha
M O
MO
Department of Surgery, Federal University of São Paulo, Escola Paulista de Medicina, São Paulo, Brazil. taha@uol.com.br
Simões
M J
MJ
Haddad
M A
MA
Capelato
R C
RC
Budny
N
N
Matsumoto
A H
AH
Soares
P C M
PC
Santos
W M
WM
Armeato
G D
GD
Araki
C M
CM
Gomes
J S M
JS
Magalhães
K G
KG
Tersariol
I L S
IL
Monteiro
H P
HP
Oliveira-Júnior
I S
IS
Oliveira
I
I
Jurkiewicz
A
A
Caricati-Neto
A
A
D000410:Alanine Transaminase / Q000097:blood / Q000187:drug effects; D000818:Animals; D001120:Arginine / Q000627:therapeutic use*; D001219:Aspartate Aminotransferases / Q000097:blood / Q000187:drug effects; D007770:L-Lactate Dehydrogenase / Q000097:blood / Q000187:drug effects; D008102:Liver Circulation / Q000187:drug effects; D008107:Liver Diseases / Q000517:prevention & control*; D008297:Male; D009569:Nitric Oxide / Q000378:metabolism; D011817:Rabbits; D015427:Reperfusion Injury / Q000201:enzymology / Q000473:pathology / Q000517:prevention & control*; D014661:Vasoconstriction / Q000187:drug effects
D009569:Nitric Oxide; D001120:Arginine; D007770:L-Lactate Dehydrogenase; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase