Mass spectrometry assisted lithography for the patterning of cell adhesion ligands on self-assembled monolayers.
Pattern of events: A simple and flexible method has been developed for patterning cell adhesion ligands. Locally erasing self-assembled monolayers with tri(ethyleneglycol) groups on a gold substrate by using a MALDI-TOF MS nitrogen laser and filling the exposed gold surface with an alkanethiol presenting carboxylic acid groups enables subsequent immobilization of maleimide and a cell adhesion peptide, which can then recognize cells (see scheme).
Kim
Young-Kwan
YK
Department of Chemistry and School of Molecular Science (BK21), Institute for the BioCentury, Korea Advanced Institute of Science and Technology, 373-1 Guseong-dong, Yuseong-gu, Daejeon 305-701, Korea.
Ryoo
Soo-Ryoon
SR
Kwack
Sul-Jin
SJ
Min
Dal-Hee
DH
D002448:Cell Adhesion; D015815:Cell Adhesion Molecules / Q000737:chemistry*; D005026:Ethylene Glycols / Q000737:chemistry; D006046:Gold / Q000737:chemistry; D055767:Immobilized Proteins / Q000737:chemistry*; D008024:Ligands; D008301:Maleimides / Q000737:chemistry; D009842:Oligopeptides / Q000737:chemistry*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D013499:Surface Properties
D015815:Cell Adhesion Molecules; D005026:Ethylene Glycols; D055767:Immobilized Proteins; D008024:Ligands; D008301:Maleimides; D009842:Oligopeptides; C043592:maleimide; D006046:Gold