An acid-activated nucleobase transporter from Leishmania major.
Parasitic protozoa are unable to synthesize purines de novo and must import preformed purine nucleobases or nucleosides from their hosts. Leishmania major expresses two purine nucleobase transporters, LmaNT3 and LmaNT4. Previous studies revealed that at neutral pH, LmaNT3 is a broad specificity, high affinity nucleobase transporter, whereas LmaNT4 mediates the uptake of only adenine. Because LmaNT4 is required for optimal viability of the amastigote stage of the parasite that lives within acidified phagolysomal vesicles of mammalian macrophages, the function of this permease was examined under acidic pH conditions. At acidic pH, LmaNT4 acquires the ability to transport adenine, hypoxanthine, guanine, and xanthine with Km values in the micromolar range, indicating that this transporter is activated at low pH. Thus, LmaNT4 is an acid-activated purine nucleobase transporter that functions optimally under the physiological conditions the parasite is exposed to in the macrophage phagolysosome. In contrast, LmaNT3 functions optimally at neutral pH. Two-electrode voltage clamp experiments performed on LmaNT3 and LmaNT4 expressed in Xenopus oocytes revealed substrate-induced inward directed currents at acidic pH, and application of substrates induced acidification of the oocyte cytosol. These observations imply that LmaNT3 and LmaNT4 are nucleobase/proton symporters.
From the Department of Molecular Microbiology and Immunology, Portland, Oregon 97239.
Diana
D
Ortiz
From the Department of Molecular Microbiology and Immunology, Portland, Oregon 97239.
Marco A
MA
Sanchez
Neuroscience Institute, Oregon Health & Science University, Portland, Oregon 97239.
Hans P
HP
Koch
Neuroscience Institute, Oregon Health & Science University, Portland, Oregon 97239.
H Peter
HP
Larsson
From the Department of Molecular Microbiology and Immunology, Portland, Oregon 97239. Electronic address: landfear@ohsu.edu.
Scott M
SM
Landfear
D000143:Acids / Q000378:metabolism*; D000225:Adenine / Q000493:pharmacokinetics; D000493:Allopurinol / Q000493:pharmacokinetics; D000818:Animals; D000963:Antimetabolites / Q000493:pharmacokinetics; D006863:Hydrogen-Ion Concentration; D019271:Hypoxanthine / Q000493:pharmacokinetics; D018320:Leishmania major / Q000502:physiology*; D026901:Membrane Transport Proteins / Q000378:metabolism*; D033702:Nucleobase Transport Proteins / Q000378:metabolism*; D009865:Oocytes / Q000502:physiology; D018408:Patch-Clamp Techniques; D010588:Phagosomes / Q000502:physiology; D014316:Tritium; D014981:Xenopus
D000143:Acids; D000963:Antimetabolites; D026901:Membrane Transport Proteins; D033702:Nucleobase Transport Proteins; D014316:Tritium; D019271:Hypoxanthine; D000493:Allopurinol; D000225:Adenine