Intracellular delivery of nanogel-quantum dot hybrid nanoparticles into human periodontal ligament cells.
Nanogels of cholesterol-bearing pullulan modified with amino-groups (CHPNH(2)) were utilized as a career to introduce quantum dots (QDs) into periodontal ligament (PDL) cells. Nanogel-QD effectively labeled PDL cells over 3 weeks. These results indicate that CHPNH(2)nanogels have a potential as a research tool in the studies of intracellular delivery system.
Section of Periodontology, Department of Hard Tissue Engineering, Graduate School, Tokyo Medical and Dental University 1-5-45 Yushima, Bunkyo-ku, Tokyo, 113-8549, Japan. fukui.peri@tmd.ac.jp
Tomoe
T
Fukui
Hiroaki
H
Kobayashi
Urara
U
Hasegawa
Toshiyuki
T
Nagasawa
Kazunari
K
Akiyoshi
Isao
I
Ishikawa
D002478:Cells, Cultured; D002784:Cholesterol / Q000008:administration & dosage*; D016503:Drug Delivery Systems; D005936:Glucans / Q000008:administration & dosage*; D006801:Humans; D020100:Hydrogels / Q000737:chemistry*; D036103:Nanotechnology / Q000379:methods; D010513:Periodontal Ligament / Q000166:cytology / Q000378:metabolism; D045663:Quantum Dots; D013997:Time Factors
D005936:Glucans; D020100:Hydrogels; C009109:pullulan; D002784:Cholesterol