Surface plasmon resonance assay for chloramphenicol without surface regeneration.
A surface plasmon resonance (SPR) assay without surface regeneration was developed for rapid and sensitive detection of chloramphenicol (CAP). A CAP-amine derivative was synthesized using a polyethylene glycol chain attached to the CAP through a carbamate linkage and immobilized onto a Biacore dextran surface. This chemically modified surface significantly changed the binding behavior between antibody and CAP, shown by both fast association and fast dissociation rates, and created a rapid and sensitive SPR immunoassay of the CAP without any regeneration. The limits of detection achieved for CAP were 32.2 pg/ml in aqueous buffer and 42.4 pg/ml in honey-spiked samples.
Biosensors and Biomeasurement, New Zealand Institute for Plant and Food Research, Hamilton, Waikato 2001, New Zealand.
Jing
J
Yuan
James
J
Addo
Marie-Isabel
MI
Aguilar
Yinqiu
Y
Wu
D000906:Antibodies / Q000737:chemistry / Q000276:immunology; D002701:Chloramphenicol / Q000032:analysis* / Q000737:chemistry; D016264:Dextran Sulfate / Q000737:chemistry; D007118:Immunoassay / Q000379:methods*; D011092:Polyethylene Glycols / Q000737:chemistry; D020349:Surface Plasmon Resonance / Q000379:methods*; D013499:Surface Properties
D000906:Antibodies; D011092:Polyethylene Glycols; D002701:Chloramphenicol; D016264:Dextran Sulfate