Actinomycetes for marine drug discovery isolated from mangrove soils and plants in China.
The mangrove ecosystem is a largely unexplored source for actinomycetes with the potential to produce biologically active secondary metabolites. Consequently, we set out to isolate, characterize and screen actinomycetes from soil and plant material collected from eight mangrove sites in China. Over 2,000 actinomycetes were isolated and of these approximately 20%, 5%, and 10% inhibited the growth of Human Colon Tumor 116 cells, Candida albicans and Staphylococcus aureus, respectively, while 3% inhibited protein tyrosine phosphatase 1B (PTP1B), a protein related to diabetes. In addition, nine isolates inhibited aurora kinase A, an anti-cancer related protein, and three inhibited caspase 3, a protein related to neurodegenerative diseases. Representative bioactive isolates were characterized using genotypic and phenotypic procedures and classified to thirteen genera, notably to the genera Micromonospora and Streptomyces. Actinomycetes showing cytotoxic activity were assigned to seven genera whereas only Micromonospora and Streptomyces strains showed anti-PTP1B activity. We conclude that actinomycetes isolated from mangrove habitats are a potentially rich source for the discovery of anti-infection and anti-tumor compounds, and of agents for treating neurodegenerative diseases and diabetes.
Hong
Kui
K
Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agriculture Sciences, Haikou 571101, PR China. k1022@163.net
Gao
An-Hui
AH
Xie
Qing-Yi
QY
Gao
Hao
H
Zhuang
Ling
L
Lin
Hai-Peng
HP
Yu
Hai-Ping
HP
Li
Jia
J
Yao
Xin-Sheng
XS
Goodfellow
Michael
M
Ruan
Ji-Sheng
JS
D039903:Actinobacteria / Q000737:chemistry* / Q000145:classification / Q000302:isolation & purification*; D000818:Animals; D000890:Anti-Infective Agents / Q000302:isolation & purification / Q000494:pharmacology; D000970:Antineoplastic Agents / Q000302:isolation & purification / Q000494:pharmacology; D064096:Aurora Kinase A; D064127:Aurora Kinases; D002176:Candida albicans / Q000187:drug effects; D061945:Caspase Inhibitors; D002681:China; D055808:Drug Discovery; D004354:Drug Screening Assays, Antitumor; D017753:Ecosystem; D007004:Hypoglycemic Agents / Q000302:isolation & purification / Q000494:pharmacology; D019684:Magnoliopsida / Q000382:microbiology*; D008828:Microbiological Techniques; D010802:Phylogeny; D017346:Protein Serine-Threonine Kinases / Q000037:antagonists & inhibitors; D054562:Protein Tyrosine Phosphatase, Non-Receptor Type 1 / Q000037:antagonists & inhibitors; D012988:Soil Microbiology; D013211:Staphylococcus aureus / Q000187:drug effects; D014407:Tumor Cells, Cultured
D000890:Anti-Infective Agents; D000970:Antineoplastic Agents; D061945:Caspase Inhibitors; D007004:Hypoglycemic Agents; C576917:AURKA protein, human; D064096:Aurora Kinase A; D064127:Aurora Kinases; D017346:Protein Serine-Threonine Kinases; D054562:Protein Tyrosine Phosphatase, Non-Receptor Type 1