The PI(3,5)P2 and PI(4,5)P2 interactomes.
A comprehensive analysis of the phosphoinositide interactome has been performed using analogues of PI(3,5)P2 and PI(4,5)P2 phosphatidyl phospholipids which were immobilized onto Affi-10 beads or incorporated into liposomes for use as affinity absorbents with cytosolic extracts from colonic carcinoma cell lines. Affinity/LC/MS/MS experiments allowed identification of 388 proteins/protein complexes that appeared to interact specifically with the phosphoinositide targets: a number of novel potential phosphoinositide interacting proteins have been identified.
Ludwig Institute for Cancer Research, Melbourne Tumour Biology Branch, Royal Melbourne Hospital, Parkville Victoria 3052, Australia. Bruno.catimel@ludwig.edu.au
Bruno
B
Catimel
Christine
C
Schieber
Melanie
M
Condron
Heather
H
Patsiouras
Lisa
L
Connolly
Jenny
J
Catimel
Edouard C
EC
Nice
Antony W
AW
Burgess
Andrew B
AB
Holmes
D045744:Cell Line, Tumor; D002852:Chromatography, Ion Exchange / Q000379:methods; D003600:Cytosol / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D015971:Gene Expression Regulation, Enzymologic; D006801:Humans; D008081:Liposomes / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy; D010455:Peptides / Q000737:chemistry; D019269:Phosphatidylinositol 4,5-Diphosphate / Q000737:chemistry* / Q000378:metabolism; D018129:Phosphatidylinositol Phosphates / Q000737:chemistry* / Q000378:metabolism; D010743:Phospholipids / Q000737:chemistry; D010766:Phosphorylation; D020543:Proteome; D040901:Proteomics / Q000379:methods*
D008081:Liposomes; D010455:Peptides; D019269:Phosphatidylinositol 4,5-Diphosphate; D018129:Phosphatidylinositol Phosphates; D010743:Phospholipids; D020543:Proteome; C106336:phosphatidylinositol 3,5-diphosphate