Fixed-time artificial insemination with estradiol and progesterone for Bos indicus cows I: basis for development of protocols.
Five experiments were conducted on commercial farms in Brazil aiming to develop a fixed-time artificial insemination (TAI) protocol that achieved pregnancy rates between 40% and 55% in Bos indicus cows. These studies resulted in the development of the following protocol: insertion of an intravaginal device containing 1.9 g of progesterone (CIDR) plus 2.0mg im estradiol benzoate on Day 0; 12.5mg im dinoprost tromethamine on Day 7 in cycling cows or on Day 9 in anestrous cows; CIDR withdrawal plus 0.5mg im estradiol cypionate plus temporary calf removal on Day 9; TAI (48h after CIDR withdrawal) plus reuniting of calves with their dams on Day 11. Reduced dose of prostaglandin F(2alpha) (PGF(2alpha); 12.5mg im dinoprost tromethamine) effectively caused luteolysis. In cycling cows, fertility was greater when the treatment with PGF(2alpha) was administered on Day 7 than on Day 9, but in anestrous cows, no effects of time of the PGF(2alpha) treatment were found. Estradiol cypionate effectively replaced estradiol benzoate or gonadotropin-releasing hormone as the ovulatory stimulus, reducing labor and cost. In this protocol, CIDR inserts were successfully used four times (9 d each use) with no detrimental effects on fertility.
Meneghetti
M
M
Departamento de Produção Animal, Faculdade de Medicina Veterinária e Zootecnia-UNESP, Botucatu-SP, Brazil.
Sá Filho
O G
OG
Peres
R F G
RF
Lamb
G C
GC
Vasconcelos
J L M
JL
D000282:Administration, Intravaginal; D000818:Animals; D002417:Cattle / Q000502:physiology*; D015237:Dinoprost / Q000008:administration & dosage; D004958:Estradiol / Q000008:administration & dosage* / Q000031:analogs & derivatives; D005260:Female; D007987:Gonadotropin-Releasing Hormone / Q000008:administration & dosage; D007315:Insemination, Artificial / Q000379:methods / Q000662:veterinary*; D003341:Luteolysis; D006080:Ovarian Follicle / Q000033:anatomy & histology; D010062:Ovulation Induction / Q000379:methods / Q000662:veterinary; D011247:Pregnancy; D011374:Progesterone / Q000008:administration & dosage*
C074283:estradiol 3-benzoate; D007987:Gonadotropin-Releasing Hormone; D011374:Progesterone; D004958:Estradiol; C007630:estradiol 17 beta-cypionate; D015237:Dinoprost