Profiling of infection specific mRNA transcripts of the European seabass Dicentrarchus labrax.
BACKGROUND
The European seabass (Dicentrarchus labrax), one of the most extensively cultured species in European aquaculture productions, is, along with the gilthead sea bream (Sparus aurata), a prospective model species for the Perciformes which includes several other commercially important species. Massive mortalities may be caused by bacterial or viral infections in intensive aquaculture production. Revealing transcripts involved in immune response and studying their relative expression enhances the understanding of the immune response mechanism and consequently also the creation of vaccines. The analysis of expressed sequence tags (EST) is an efficient and easy approach for gene discovery, comparative genomics and for examining gene expression in specific tissues in a qualitative and quantitative way.


RESULTS
Here we describe the construction, analysis and comparison of a total of ten cDNA libraries, six from different tissues infected with V. anguillarum (liver, spleen, head kidney, gill, peritoneal exudates and intestine) and four cDNA libraries from different tissues infected with Nodavirus (liver, spleen, head kidney and brain). In total 9605 sequences representing 3075 (32%) unique sequences (set of sequences obtained after clustering) were obtained and analysed. Among the sequences several immune-related proteins were identified for the first time in the order of Perciformes as well as in Teleostei.


CONCLUSIONS
The present study provides new information to the Gene Index of seabass. It gives a unigene set that will make a significant contribution to functional genomic studies and to studies of differential gene expression in relation to the immune system. In addition some of the potentially interesting genes identified by in silico analysis and confirmed by real-time PCR are putative biomarkers for bacterial and viral infections in fish.
Institute of Marine Biology and Genetics, Hellenic Center of Marine Research, Iraklio, Crete, Greece. sarris@her.hcmr.gr
Elena
E
Sarropoulou
Pilar
P
Sepulcre
Laura
L
Poisa-Beiro
Victoriano
V
Mulero
José
J
Meseguer
Antonio
A
Figueras
Beatriz
B
Novoa
Vasso
V
Terzoglou
Richard
R
Reinhardt
Antonios
A
Magoulas
Georgios
G
Kotoulas
D000818:Animals; D001492:Bass / Q000235:genetics* / Q000276:immunology*; D020224:Expressed Sequence Tags; D005393:Fish Diseases / Q000235:genetics / Q000276:immunology; D020869:Gene Expression Profiling; D015723:Gene Library; D018895:Microsatellite Repeats; D030381:Nodaviridae; D020411:Oligonucleotide Array Sequence Analysis; D012333:RNA, Messenger / Q000235:genetics* / Q000276:immunology; D017422:Sequence Analysis, DNA; D014733:Vibrio
D012333:RNA, Messenger