High-resolution mosaic imaging with multifocal, multiphoton photon-counting microscopy.
High-resolution mosaic imaging is performed for the first time to our knowledge with a multifocal, multiphoton, photon-counting imaging system. We present a novel design consisting of a home-built femtosecond Yb-doped KGdWO(4) laser with an optical multiplexer, which is coupled with a commercial Olympus IX-71 microscope frame. Photon counting is performed using single-element detectors and an inexpensive electronic demultiplexer and counters.
Chandler
Eric
E
Center for Microintegrated Optics for Advanced Biological Control, Department of Physics, Colorado School of Mines, 1500 Illinois Street, Golden, Colorado 80401, USA. echandle@mines.edu
Hoover
Erich
E
Field
Jeff
J
Sheetz
Kraig
K
Amir
Wafa
W
Carriles
Ramon
R
Ding
Shi-You
SY
Squier
Jeff
J
D007834:Lasers; D008853:Microscopy / Q000379:methods*; D017785:Photons