Characterization of a novel ghrelin cell reporter mouse.
Ghrelin is a hormone that influences many physiological processes and behaviors, such as food intake, insulin and growth hormone release, and a coordinated response to chronic stress. However, little is known about the molecular pathways governing ghrelin release and ghrelin cell function. To better study ghrelin cell physiology, we have generated several transgenic mouse lines expressing humanized Renilla reniformis green fluorescent protein (hrGFP) under the control of the mouse ghrelin promoter. hrGFP expression was especially abundant in the gastric oxyntic mucosa, in a pattern mirroring that of ghrelin immunoreactivity and ghrelin mRNA. hrGFP expression also was observed in the duodenum, but not in the brain, pancreatic islet, or testis. In addition, we used fluorescent activated cell sorting (FACS) to collect and partially characterize highly enriched populations of gastric ghrelin cells. We suggest that these novel ghrelin-hrGFP transgenic mice will serve as useful tools to better understand ghrelin cell physiology.
Sakata
Ichiro
I
Department of Internal Medicine, The University of Texas Southwestern Medical Center, 5323 Harry Hines Blvd, Dallas, TX 75390-9077, USA.
Nakano
Yoshihide
Y
Osborne-Lawrence
Sherri
S
Rovinsky
Sherry A
SA
Lee
Charlotte E
CE
Perello
Mario
M
Anderson
Jason G
JG
Coppari
Roberto
R
Xiao
Guanghua
G
Lowell
Bradford B
BB
Elmquist
Joel K
JK
Zigman
Jeffrey M
JM
D000818:Animals; D001921:Brain / Q000378:metabolism; D004386:Duodenum / Q000378:metabolism; D005434:Flow Cytometry; D005753:Gastric Mucosa / Q000378:metabolism; D054439:Ghrelin / Q000097:blood / Q000378:metabolism*; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D007515:Islets of Langerhans / Q000378:metabolism; D008297:Male; D051379:Mice; D008822:Mice, Transgenic; D008856:Microscopy, Fluorescence; D011401:Promoter Regions, Genetic; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013737:Testis / Q000378:metabolism
D054439:Ghrelin; D049452:Green Fluorescent Proteins