Light at the end of the tunnel.
Turn the light on: The synthesis, folding and maturation kinetics of individual green fluorescent protein (GFP) molecules have been studied using a cell-free transcription-translation system and single-molecule fluorescence. Ribosomes, which were labeled with a red fluorophore and tethered to a glass surface, were used to synthesize GFP Emerald molecules that become fluorescent after maturation.
Applied Laser Physics and Laser Spectroscopy and Bielefeld Institute for Biophysics and Nanoscience, Bielefeld University, Germany. heileman@physik.uni-bielefeld.de
Mike
M
Heilemann
D005453:Fluorescence; D049452:Green Fluorescent Proteins / Q000096:biosynthesis* / Q000737:chemistry*; D017510:Protein Folding; D012270:Ribosomes / Q000378:metabolism*
D049452:Green Fluorescent Proteins