Profiling genome-wide histone modifications and variants by ChIP-chip on tiling microarrays in S. cerevisiae.
Chromatin immunoprecipitation coupled to DNA microarray has become a widely used method to study transcription factors and chromatin structure. Here, we provide a detailed protocol for the localization of the variant histone Htz1 in the S. cerevisiae genome. This protocol can easily be adapted to fit other purposes such as profiling histone modifications.
Bataille
Alain R
AR
Institut de Recherches Cliniques de Montréal, 110 Avenue des Pins Ouest, Montréal, QC, Canada, H2W 1R7.
Robert
François
F
D002843:Chromatin / Q000378:metabolism; D047369:Chromatin Immunoprecipitation / Q000379:methods*; D003432:Cross-Linking Reagents / Q000494:pharmacology; D004271:DNA, Fungal / Q000302:isolation & purification; D016681:Genome, Fungal / Q000235:genetics*; D006657:Histones / Q000378:metabolism*; D009154:Mutation / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis; D016133:Polymerase Chain Reaction; D011499:Protein Processing, Post-Translational / Q000187:drug effects; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development; D012995:Solubility / Q000187:drug effects
D002843:Chromatin; D003432:Cross-Linking Reagents; D004271:DNA, Fungal; D006657:Histones