HIV type 1 group M subtype G in Cameroon: five genome sequences.
Near full-length viral genome sequences were obtained for five putative subtype G candidates identified in HIV-infected Cameroonian blood donors, based on partial genome sequences for the gag, pol, and env regions. Phylogenetic analysis of the genome sequences shows that all five strains are pure subtype G with no indication of intersubtype recombination. The Cameroon subtype G sequences did not form a geographically based subcluster and were intermixed within the subtype G branch with isolates from several different countries. HIV-1 group M subtype G accounts for only 4.5% of HIV infections in Cameroon. However, genome segments of subtype G are present in 67% of all infections and 80% of infections due to intersubtype recombinant strains in Cameroon. The addition of five subtype G genome sequences to the HIV database may contribute to a better understanding of the origins and classification of HIV-1 subtypes and CRFs.
Abbott Diagnostics, Abbott Park, Illinois 60064, USA.
Julie
J
Yamaguchi
Nicaise
N
Ndembi
Charlotte
C
Ngansop
Dora
D
Mbanya
Lazare
L
Kaptué
Lutz G
LG
Gürtler
Sushil G
SG
Devare
Catherine A
CA
Brennan
D001782:Blood Donors; D002163:Cameroon / Q000453:epidemiology; D004279:DNA, Viral / Q000032:analysis; D016679:Genome, Viral; D015658:HIV Infections / Q000453:epidemiology / Q000821:virology*; D015497:HIV-1 / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D006801:Humans; D008969:Molecular Sequence Data; D010802:Phylogeny; D011995:Recombination, Genetic; D017422:Sequence Analysis, DNA
D004279:DNA, Viral