Multiple embryo time-lapse imaging of zebrafish development.
Understanding the dynamics of developmental and cellular processes requires documentation of their changes with appropriate temporal and spatial resolution. Furthermore, simultaneous recording from a population of embryos under identical conditions allows statistical estimates of precision and variability to be made. This chapter describes a protocol for time-lapse microscopy of multiple embryos in parallel developing under tightly controlled conditions. This method is currently best suited to follow tissue-scale morphogenetic movements with temporal resolution in the minute range, for hours or even days. Applications of the method include the comparison of the dynamics of a process of interest between groups of wild-type embryos and their mutant siblings or between embryos treated with different chemical compounds. Temperature control allows for the investigation of the temperature dependence of a process of interest.
Max Planck Institute of Molecular Cell Biology and Genetics, Pfotenhauerstrasse 108, D*resden, 01307, Germany.
Leah
L
Herrgen
Christian
C
Schröter
Lola
L
Bajard
Andrew C
AC
Oates
D000818:Animals; D015509:Developmental Biology / Q000379:methods; D004625:Embryo, Nonmammalian / Q000033:anatomy & histology; D047108:Embryonic Development; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D013696:Temperature; D013997:Time Factors; D015027:Zebrafish / Q000033:anatomy & histology / Q000254:growth & development*