Biolistic gun-mediated maize genetic transformation.
Biolistic gun-mediated transformation is one of the two most effective and popular methods for introducing genes into maize. In this chapter, we describe a detailed protocol for genetic transformation of the maize genotype, Hi II. The protocol uses 0.6-microm gold particles as microcarriers and the herbicide resistance bar gene as a selective marker. Both immature zygotic embryos and immature embryo-derived callus cultures can be transformed using this protocol. To ensure successful reproduction of this protocol, we provide step-by-step laboratory transformation procedures as well as details on growing and caring for transgenic plants in the greenhouse.
Wang
Kan
K
Center for Plant Transformation, Iowa State University, Ames, IA, USA. kanwang@iastate.edu
Frame
Bronwyn
B
D019470:Biolistics / Q000379:methods*; D004274:DNA, Recombinant / Q000008:administration & dosage / Q000235:genetics; D006046:Gold; D030821:Plants, Genetically Modified; D014170:Transformation, Genetic; D003313:Zea mays / Q000196:embryology / Q000235:genetics* / Q000254:growth & development
D004274:DNA, Recombinant; D006046:Gold