AMT: preclinical pharmacology studies.
Auron-Misheil-Therapy (AMT) consisting of aqueous camomile extract supplemented with calcium, vitamins, the antihistamine chlorpheniramine and human insulin is under development as anti-cancer treatment. AMT was preclinically investigated in tumour cell lines and tumour xenografts to guide clinical phase I/II studies. AMT was tested against 56 human tumour cell lines, in a clonogenic assay in 98 patient-derived xenografts and in in vivo studies. AMT showed in vitro cytotoxic activity with highest susceptibility in cervical cancer, glioblastoma and colon cancers. In the clonogenic assay, anti- cancer activity of AMT was most active in cervical and uterine tumours, in colon cancer, glioblastoma, leukaemia, melanoma and pancreatic cancer. In vivo, AMT showed slight activity in tumour xenograft models of colon and mammary cancer. It also showed immune stimulatory effects by induction of IL-6- and TNF-alpha secretion in human PBMCs. The immune stimulatory potential of AMT, together with slight anti-tumour efficacy observed in the present study, indicates a role of AMT in tumour therapy.
Auron Healthcare GmbH, 79100 Freiburg, Germany.
Faiz
F
Niazi
Joachim
J
Drevs
Klaus
K
Diergarten
Annette
A
Dorn
Armin
A
Maier
Heinz Herbert
HH
Fiebig
Eddy
E
Bruyns
Jürgen
J
Scheele
D000818:Animals; D000971:Antineoplastic Combined Chemotherapy Protocols / Q000494:pharmacology* / Q000627:therapeutic use; D002118:Calcium / Q000494:pharmacology / Q000627:therapeutic use; D002478:Cells, Cultured; D020843:Chamomile / Q000737:chemistry*; D002744:Chlorpheniramine / Q000494:pharmacology / Q000627:therapeutic use; D004353:Drug Evaluation, Preclinical; D006801:Humans; D007328:Insulin / Q000494:pharmacology / Q000627:therapeutic use; D051379:Mice; D009369:Neoplasms / Q000188:drug therapy / Q000473:pathology*; D009928:Organ Specificity / Q000187:drug effects; D008517:Phytotherapy / Q000379:methods; D010936:Plant Extracts / Q000494:pharmacology* / Q000627:therapeutic use; D016896:Treatment Outcome
C542427:Auron-Misheil-Therapy; D007328:Insulin; D010936:Plant Extracts; D002744:Chlorpheniramine; D002118:Calcium