Metabolic changes in flatfish hepatic tumours revealed by NMR-based metabolomics and metabolic correlation networks.
Histopathologically well-characterized fish liver was analyzed by 800 MHz 1H NMR metabolomics to identify metabolic changes between healthy and tumor tissue. Data were analyzed by multivariate statistics and metabolic correlation networks, and results revealed elevated anaerobic metabolism and reduced choline metabolism in tumor tissue. Significant negative correlations were observed between alanine-acetate (p = 3.0 x 10(-5)) and between proline-acetate (p = 0.003) in tumors only, suggesting alanine and proline are utilized as alternative energy sources in flatfish liver tumors.
School of Biosciences, University of Birmingham, Edgbaston, Birmingham B15 2TT, United Kingdom.
Andrew D
AD
Southam
John M
JM
Easton
Grant D
GD
Stentiford
Christian
C
Ludwig
Theodoros N
TN
Arvanitis
Mark R
MR
Viant
D000236:Adenoma / Q000378:metabolism*; D000818:Animals; D002794:Choline / Q000378:metabolism; D019295:Computational Biology / Q000379:methods*; D004777:Environment; D005189:False Positive Reactions; D005412:Flatfishes; D015972:Gene Expression Regulation, Neoplastic; D008099:Liver / Q000378:metabolism; D008113:Liver Neoplasms / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D055432:Metabolomics; D020543:Proteome; D040901:Proteomics / Q000379:methods*
D020543:Proteome; D002794:Choline