Purification and characterization of an extracellular laccase from Phlebia radiata strain BP-11-2 that decolorizes fungal melanin.
Phlebia radiata strain BP-12-2, isolated from forest soil, decolorized fungal melanin and produced an extracellular laccase in culture. The enzyme was purified to homogeneity and characterized. It showed no absorption bands in the ultraviolet above 300 nm or visible regions. It differed from the reported laccases in substrate specificity, kinetic parameters, and NH2-terminal amino acid sequences.
Department of Applied Biological Chemistry, Graduate School of Agricultural Science, Kobe University, Rokko, Kobe 657-8501, Japan.
Shuji
S
Kaneko
Minyi
M
Cheng
Hiroshi
H
Murai
Shinji
S
Takenaka
Shuichiro
S
Murakami
Kenji
K
Aoki
D000595:Amino Acid Sequence; D001487:Basidiomycota / Q000145:classification / Q000166:cytology / Q000201:enzymology*; D002963:Cladosporium / Q000378:metabolism*; D005110:Extracellular Space / Q000201:enzymology*; D007700:Kinetics; D042845:Laccase / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism*; D008543:Melanins / Q000378:metabolism*; D010858:Pigmentation; D013379:Substrate Specificity
D008543:Melanins; D042845:Laccase