Macrophage-mediated erosion of gamma irradiated poly(trimethylene carbonate) films.
A macrophage culture model was used to investigate the erosion of gamma irradiated poly(trimethylene carbonate) (PTMC) films. When the PTMC films were incubated in the culture medium, but physically separated from the cells by a membrane, no erosion occurred. In contrast, when the J774A macrophages were directly cultured on PTMC films, they adhered to the films and were found to have eroded the polymer surface. Macrophages adhered to gamma irradiated poly(epsilon-caprolactone) (PCL) controls as well, but to a lesser extent than to the PTMC films. In this case, no signs of erosion were observed. Human skin fibroblasts cultured on PTMC and PCL films as controls also adhered to the films but did not erode the surfaces. The effect of enzymes and reactive oxygen species that can be secreted by macrophages on the erosion process was assessed using aqueous solutions of cholesterol esterase, lipoprotein lipase, esterase, potassium superoxide, and hydrogen peroxide. The PTMC films eroded in aqueous enzyme solutions as well as in aqueous superoxide solutions. Cholesterol esterase and superoxide anion radicals seem to be most involved in the macrophage-mediated erosion of PTMC. This macrophage culture model is useful in assessing the influence of macrophages on the in vivo biodegradability of polymers and in elucidating the biodegradation mechanisms involved.
Institute for Biomedical Technology (BMTI), Department of Polymer Chemistry and Biomaterials, Faculty of Science and Technology, University of Twente, P.O. Box 217, 7500 AE Enschede, The Netherlands.
Erhan
E
Bat
Theo G
TG
van Kooten
Jan
J
Feijen
Dirk W
DW
Grijpma
D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry / Q000378:metabolism / Q000528:radiation effects; D002448:Cell Adhesion / Q000502:physiology; D018929:Cell Culture Techniques; D002478:Cells, Cultured; D004146:Dioxanes / Q000737:chemistry / Q000378:metabolism / Q000528:radiation effects; D005720:Gamma Rays; D006801:Humans; D008264:Macrophages / Q000378:metabolism* / Q000648:ultrastructure; D008422:Materials Testing; D011091:Polyesters / Q000737:chemistry / Q000378:metabolism; D011108:Polymers / Q000737:chemistry / Q000378:metabolism / Q000528:radiation effects; D017382:Reactive Oxygen Species / Q000378:metabolism; D013499:Surface Properties
D001672:Biocompatible Materials; D004146:Dioxanes; D011091:Polyesters; D011108:Polymers; D017382:Reactive Oxygen Species; C016240:polycaprolactone; C443643:trimethylene carbonate