Baculovirus-mediated gene transfer into mesenchymal stem cells.
Mesenchymal stem cells (MSCs) have drawn considerable attention as vehicles for cell- and gene-based therapies. Additionally, baculovirus has emerged as a novel gene therapy vector because of its large cloning capacity for insertion of multiple genes, its minimal cytotoxic effects, and its inability to replicate in mammalian cells. These features have prompted efforts to employ baculovirus vectors carrying mammalian expression cassettes for gene delivery into MSCs. This chapter demonstrates the use of GFP expression to monitor baculovirus-mediated gene transfer into MSCs.
Tsai
Chien-Tai
CT
Department of Chemical Engineering, National Tsing Hua University, 101, Sec.2, Kuang Fu Rd, Hsinchu, 300, Taiwan.
Chuang
Ching-Kuang
CK
Hu
Yu-Chen
YC
D000818:Animals; D016367:Baculoviridae / Q000235:genetics*; D002478:Cells, Cultured; D018014:Gene Transfer Techniques; D005822:Genetic Vectors / Q000235:genetics; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000378:metabolism*; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism
D011994:Recombinant Proteins