Cytokine multiplex analysis.
The ability to monitor gene expression in experimental and clinical samples is an essential element of modern molecular biology and cell biology research. However with the advent of a systems biology approach toward understanding cell and cancer biology, analysis of expression of a single gene is no longer desirable. Today, multiplex analysis, where the expression of 8-100 genes can be monitored in one sample, has become a routine aspect of gene expression analysis. In this chapter the various assays systems commercially available for multiplex analysis of both RNA and protein will be discussed.
Laboratory of Experimental Immunology, Cancer and Inflammation Program, Center for Cancer Research, National Cancer Institute-Frederick, Frederick, MD, USA.
Howard A
HA
Young
D001681:Biological Assay / Q000295:instrumentation / Q000379:methods; D016207:Cytokines / Q000032:analysis*; D020869:Gene Expression Profiling / Q000295:instrumentation / Q000379:methods; D023281:Genomics / Q000295:instrumentation / Q000379:methods; D006801:Humans; D008863:Microspheres; D020411:Oligonucleotide Array Sequence Analysis / Q000295:instrumentation / Q000379:methods; D016133:Polymerase Chain Reaction / Q000295:instrumentation / Q000379:methods; D012313:RNA / Q000032:analysis
D016207:Cytokines; D012313:RNA