Systemic analysis of a novel coxsackievirus gene delivery system in a mouse model.
In order to systemically investigate the possibility of using coxsackievirus B3 (CVB3) to deliver foreign genes in vivo, a recombinant strain of CVB3 encoding the renilla gene (CVB3- renilla) was constructed. The recombinant CVB3 resulted in extensive and transient expression of the renilla protein within mouse organs, especially the pancreas. The level of expression was generally dependent upon the viral titer present. Moreover, the CVB3-renilla strain was completely attenuated. Interestingly, the recombinant CVB3 vector was expressed much more strongly in mouse organs than was a comparable adenoviral vector. The CVB3-renilla strain did not express the renilla gene in mice with pre-existing coxsackievirus-specific neutralizing antibodies, but direct organ-specific administration of the virus during openperitoneum surgery was able to circumvent this immunity. This coxsackievirus vector may represent a useful means for delivering and expressing foreign genes in mouse models in an acute and extensive fashion.
Kim
Yeon-Jung
YJ
Department of Biotechnology, The Catholic University of Korea, Bucheon 420-743, Korea.
Yun
Soo-Hyeon
SH
Lim
Byung-Kwan
BK
Park
Ki-Bum
KB
Na
Ha-Na
HN
Jeong
Soo-Young
SY
Kim
Dae-Sun
DS
Cho
Young-Joo
YJ
Jeon
Eun-Seok
ES
Nam
Jae-Hwan
JH
D000256:Adenoviridae / Q000235:genetics; D000818:Animals; D000914:Antibodies, Viral / Q000378:metabolism; D029822:Enterovirus B, Human / Q000502:physiology*; D018014:Gene Transfer Techniques; D017930:Genes, Reporter; D005822:Genetic Vectors / Q000008:administration & dosage / Q000096:biosynthesis; D006367:HeLa Cells; D006801:Humans; D007274:Injections, Intraperitoneal; D049410:Luciferases, Renilla / Q000008:administration & dosage / Q000096:biosynthesis; D049408:Luminescent Agents / Q000008:administration & dosage / Q000378:metabolism; D051379:Mice; D010179:Pancreas / Q000187:drug effects; D014779:Virus Replication
D000914:Antibodies, Viral; D049408:Luminescent Agents; D049410:Luciferases, Renilla