Characterization and genetic diversity of the sturgeon Acipenser schrenskii Ig heavy chain.
It is still unknown about the VH gene organization and diversity of the immunoglobulin (Ig) heavy chain locus in Amur sturgeon. In this study, Ig heavy chain alleles were cloned by RT-PCR using the specific primers. Sequence analysis showed that Amur sturgeon's VH regions belonged to the same family with higher than 90% identities of their leader peptide (LP). Moreover, a number of conserved motifs in the D segment were identified, and the variability of the CDR3 region was substantial. Further, we speculated that there were at least 12 different JH segments in the locus, contributing to the antibody repertoire of the sturgeon. The genetic diversity of the sturgeon Ig should be associated with the random rearrangement of VH, D and JH segments, action of exonuclease and insertion of N and/or probably P nucleotides at the site of rearrangement.
Wang
Di
D
Heilongjiang River Fishery Research Institute of Chinese Academy of Fishery Science, Harbin 150070, China.
Lu
Tong-yan
TY
Liu
Hong-bai
HB
D000483:Alleles; D000818:Animals; D003001:Cloning, Molecular; D019143:Evolution, Molecular; D005399:Fishes / Q000235:genetics* / Q000276:immunology*; D015322:Gene Rearrangement, B-Lymphocyte / Q000235:genetics*; D005803:Genes, Immunoglobulin; D007143:Immunoglobulin Heavy Chains / Q000235:genetics* / Q000276:immunology; D011110:Polymorphism, Genetic; D017434:Protein Structure, Tertiary; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment; D017421:Sequence Analysis
D007143:Immunoglobulin Heavy Chains