Adipose stromal cells-conditional medium protected glutamate-induced CGNs neuronal death by BDNF.
The delivery of factors secreted by adipose stromal cells (ASC) to the brain may be a viable neuroprotective therapeutic option. In this study, we investigated the bioactivity of ASC-conditioned medium (ASC-CM) in glutamate-induced neurotoxicity and found that the ASC-CM significantly blocked glutamate neurotoxicity. We identified the brain-derived neurotrophic factor (BDNF) in the ASC-CM by using Western blot and demonstrated that this activity was critical for the neuroprotective effect of ASC-CM in excytotoxicity models. Furthermore, inactivating BDNF also attenuated the suppression by ASC-CM of glutamate-induced caspase-3 activity, but not p38 phosphorylation. These findings suggest that among ASC secrete a potent combination of factors, BDNF play a major role in neuroprotection against excytotoxicity.
Department of Neurology, School of Medicine, Indiana University, Indianapolis, IN 46202, USA.
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L
Zhao
X
X
Wei
Z
Z
Ma
D
D
Feng
P
P
Tu
B H
BH
Johnstone
K L
KL
March
Y
Y
Du
D000273:Adipose Tissue / Q000378:metabolism*; D000818:Animals; D015153:Blotting, Western; D019208:Brain-Derived Neurotrophic Factor / Q000378:metabolism*; D053148:Caspase 3 / Q000378:metabolism; D016923:Cell Death / Q000187:drug effects / Q000502:physiology; D002470:Cell Survival / Q000187:drug effects / Q000502:physiology; D002478:Cells, Cultured; D002531:Cerebellum / Q000166:cytology* / Q000187:drug effects / Q000502:physiology; D017077:Culture Media, Conditioned; D018683:Excitatory Amino Acid Agents / Q000633:toxicity*; D018698:Glutamic Acid / Q000633:toxicity*; D015151:Immunoblotting; D009474:Neurons / Q000187:drug effects / Q000502:physiology*; D010766:Phosphorylation; D051381:Rats; D017207:Rats, Sprague-Dawley; D017154:Stromal Cells / Q000378:metabolism*; D048051:p38 Mitogen-Activated Protein Kinases / Q000378:metabolism
D019208:Brain-Derived Neurotrophic Factor; D017077:Culture Media, Conditioned; D018683:Excitatory Amino Acid Agents; D018698:Glutamic Acid; D048051:p38 Mitogen-Activated Protein Kinases; D053148:Caspase 3