Analysis of chlorophylls and their derivatives by matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry.
The analysis of chlorophylls and their derivatives by matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry is described. Four matrices-sinapinic acid, a-cyano-4-hydroxycinnnamic acid, terthiophene, and 3-aminoquinoline-were examined to determine optimal conditions for analysis of the molecular mass and structure of chlorophyll a as a representative chlorophyll. Among them, terthiophene was the most efficient without releasing metal ions, although it caused fragmentation of the phytol-ester linkage. Terthiophene was useful for the analyses of chlorophyll derivatives as well as porphyrin products such as 8-deethyl-8-vinyl-chlorophyll a, pheophorbide a, pyropheophorbide a, bacteriochlorophyll a esterified phytol, and protoporphyrin IX. The current method is suitable for rapid and accurate determination of the molecular mass and structure of chlorophylls and porphyrins.
Suzuki
Toshiyuki
T
Department of Biological Science, Faculty of Science, Shizuoka University, Shizuoka 422-8529, Japan.
Midonoya
Hitoshi
H
Shioi
Yuzo
Y
D002734:Chlorophyll / Q000032:analysis* / Q000737:chemistry; D003373:Coumaric Acids / Q000032:analysis / Q000737:chemistry; D011166:Porphyrins / Q000032:analysis / Q000737:chemistry; D011524:Protoporphyrins / Q000032:analysis / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D013876:Thiophenes / Q000032:analysis / Q000737:chemistry
D003373:Coumaric Acids; D011166:Porphyrins; D011524:Protoporphyrins; D013876:Thiophenes; C019101:alpha-terthienyl; D002734:Chlorophyll; C073734:sinapinic acid; C028025:protoporphyrin IX