Dibenzocyclooctadiene lignans and lanostane derivatives from the roots of Kadsura coccinea and their protective effects on primary rat hepatocyte injury induced by t-butyl hydroperoxide.
A new dibenzocyclooctadiene lignan, acetylepigomisin R ( 1), and a new 3,4-seco-lanostane-type triterpene, seco-coccinic acid F ( 2), along with three known dibenzocyclooctadiene lignans, isovaleroylbinankadsurin A ( 3), kadsuralignan J ( 4), and binankadsurin A ( 5), and one lanostane-type triterpene, 20( R),24( E)-3-oxo-9 beta-lanosta-7,24-dien-26-oic acid ( 6), were isolated from the methanol extract of the Kadsura coccinea roots. Their structures were elucidated on the basis of spectroscopic evidence including ESI-MS, HR-EI-MS, 1D and 2D NMR. The protective effects of these compounds were evaluated in primary cultured rat hepatocytes intoxicated with 1.2 mM T-butyl hydroperoxide. Compounds 1, 3, and 5 showed protective effects with ED (50) values of 135.7, 26.1, and 79.3 microM, respectively.
Ban
Ninh Khac
NK
Institute of Ecology and Biological Resources, Vietnam Academy of Science and Technology (VAST), Hanoi, Vietnam.
Thanh
Bui Van
BV
Kiem
Phan Van
PV
Minh
Chau Van
CV
Cuong
Nguyen Xuan
NX
Nhiem
Nguyen Xuan
NX
Huong
Hoang Thanh
HT
Anh
Ha Tuan
HT
Park
Eun-Jeon
EJ
Sohn
Dong Hwan
DH
Kim
Young Ho
YH
D000818:Animals; D002478:Cells, Cultured; D034242:Cyclooctanes / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D022781:Hepatocytes / Q000187:drug effects*; D006861:Hydrogen Peroxide; D032135:Kadsura / Q000737:chemistry*; D017705:Lignans / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D019906:Nuclear Magnetic Resonance, Biomolecular; D018517:Plant Roots / Q000737:chemistry; D020011:Protective Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D051381:Rats; D021241:Spectrometry, Mass, Electrospray Ionization; D014315:Triterpenes / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*
D034242:Cyclooctanes; D017705:Lignans; D020011:Protective Agents; D014315:Triterpenes; C510994:dibenzocyclooctadiene lignan; D006861:Hydrogen Peroxide