Quantification of aromatic oxygenase genes to evaluate enhanced bioremediation by oxygen releasing materials at a gasoline-contaminated site.
Subsurface injection of oxygen-releasing materials (ORMs) is frequently performed at petroleum-contaminated sites to stimulate aerobic bioremediation of benzene, toluene, ethylbenzene, and xylenes (BTEX). In this study, qPCR enumeration of aromatic oxygenase genes and PCR-DGGE profiles of bacterial 16S rRNA genes were combined with groundwater monitoring to determine the impact of ORM injection on BTEX bioremediation at a gasoline-contaminated site. Prior to injection, BTEX concentrations were greater than 3 mg/L and DO levels were typically lessthan 2 mg/L, butphenol hydroxylase (PHE) and ring-hydroxylating toluene monooxygenase (RMO) genes were detected in impacted wells indicating the potential for aerobic BTEX biodegradation. Following injection, DO increased, BTEX concentrations decreased substantially, and PHE and RMO genes copies increased by 1-3 orders of magnitude. In addition, naphthalene dioxygenase (NAH) and xylene monooxygenase (TOL) genes were intermittently detected during periods of increased DO. Following depletion of the ORM, DO decreased, BTEX concentrations rebounded, and oxygenase genes were no longer detected. Temporal changes in PCR-DGGE microbial community profiles reflected the dynamic changes in subsurface conditions. Overall, the combination of chemical and geochemical analyses with quantification of aromatic oxygenase genes demonstrated that injection stimulated BTEX biodegradation until the ORM was depleted.
Nebe
Jennifer
J
Department of Agronomy, Purdue University, West Lafayette, Indiana 47907-2054, USA.
Baldwin
Brett R
BR
Kassab
Raymond L
RL
Nies
Loring
L
Nakatsu
Cindy H
CH
D000332:Aerobiosis; D001554:Benzene / Q000737:chemistry; D001555:Benzene Derivatives / Q000737:chemistry; D001673:Biodegradation, Environmental; D000077463:Carbamide Peroxide; D004338:Drug Combinations; D004784:Environmental Monitoring; D005742:Gasoline / Q000032:analysis*; D010105:Oxygenases / Q000378:metabolism*; D010545:Peroxides / Q000378:metabolism*; D012987:Soil / Q000032:analysis; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000737:chemistry* / Q000378:metabolism; D013997:Time Factors; D014050:Toluene / Q000737:chemistry; D014508:Urea / Q000031:analogs & derivatives* / Q000378:metabolism; D014992:Xylenes / Q000737:chemistry
D001555:Benzene Derivatives; D004338:Drug Combinations; D005742:Gasoline; D010545:Peroxides; D012987:Soil; D012989:Soil Pollutants; D014992:Xylenes; D000077463:Carbamide Peroxide; D014050:Toluene; D014508:Urea; D010105:Oxygenases; D001554:Benzene; C004912:ethylbenzene