Light at the end of the tunnel.
Turn the light on: The synthesis, folding and maturation kinetics of individual green fluorescent protein (GFP) molecules have been studied using a cell-free transcription-translation system and single-molecule fluorescence. Ribosomes, which were labeled with a red fluorophore and tethered to a glass surface, were used to synthesize GFP Emerald molecules that become fluorescent after maturation.
Heilemann
Mike
M
Applied Laser Physics and Laser Spectroscopy and Bielefeld Institute for Biophysics and Nanoscience, Bielefeld University, Germany. heileman@physik.uni-bielefeld.de
D005453:Fluorescence; D049452:Green Fluorescent Proteins / Q000096:biosynthesis* / Q000737:chemistry*; D017510:Protein Folding; D012270:Ribosomes / Q000378:metabolism*
D049452:Green Fluorescent Proteins