Luteolin inhibits adipogenic differentiation by regulating PPARgamma activation.
Luteolin (3',4',5,7-tetrahydroxyflavone), a flavonoid, has been known to possess antimutagenic, antitumorigenic, antioxidant, and anti-inflammatory properties. In this study, we investigated the role of luteolin in the regulation of adipogenic differentiation in 3T3-L1 preadipocytes. Luteolin inhibited intracellular triglyceride accumulation in a dose-dependent manner without cytotoxicity. Western blot and reverse transcription-polymerase chain reaction analyses showed that this inhibition was accompanied by attenuated expression of the adipogenic transcription factors: peroxisome proliferator-activated receptor gamma (PPARgamma) and CCAAT/enhancer-binding protein alpha. Luteolin inhibited the PPARgamma transactivation stimulated by rosiglitazone, a synthetic agonist, in COS-7 cells and inhibited rosiglitazone-induced adipogenic differentiation in 3T3-L1 cells. These data suggest that luteolin exerts antiadipogenic effects by suppressing adipogenic transcription factors and by inhibiting the transactivation of PPARgamma.
Department of Biogeron Food Technology, Korea Food Research Institute, Pundang-ku, Songnam, Kyongki-do, Republic of Korea.
Hee-Sook
HS
Park
Soon-Hee
SH
Kim
Young Sup
YS
Kim
Shi Yong
SY
Ryu
Jin-Taek
JT
Hwang
Hye Jeong
HJ
Yang
Gun-Hee
GH
Kim
Dae Young
DY
Kwon
Myung-Sunny
MS
Kim
D041721:3T3-L1 Cells; D017667:Adipocytes / Q000187:drug effects; D000818:Animals; D022763:CCAAT-Enhancer-Binding Protein-alpha / Q000096:biosynthesis; D019556:COS Cells; D002454:Cell Differentiation / Q000187:drug effects*; D002522:Chlorocebus aethiops; D047311:Luteolin / Q000494:pharmacology*; D051379:Mice; D047495:PPAR gamma / Q000187:drug effects / Q000378:metabolism*; D000077154:Rosiglitazone; D045162:Thiazolidinediones / Q000037:antagonists & inhibitors
D022763:CCAAT-Enhancer-Binding Protein-alpha; D047495:PPAR gamma; D045162:Thiazolidinediones; D000077154:Rosiglitazone; D047311:Luteolin