Design and synthesis of substrates for model ribosomal reactions.
2'/3'-O-[Bz(NO(2))-Orn(Boc)]-5'-O-Piv-Ado (1) and its deoxy analog: 3'-O-[Bz(NO(2))-Orn(Boc)]-5'-O-Piv-2'-dAdo (2) were designed and synthesized as substrates for the model ribosome reaction we used to demonstrate the crucial role of A76 2'-OH of peptidyl-tRNA in the rate acceleration of peptide bond formation during protein biosynthesis.
Laboratory of BioCatalysis, Institute of Organic Chemistry, Bulgarian Academy of Sciences, Sofia 1113, Bulgaria. stanly_chem2002@yahoo.com
Stanislav G
SG
Bayryamov
Nikolay G
NG
Vassilev
Miroslav A
MA
Rangelov
Aneta P
AP
Mladjova
Dimiter D
DD
Petkov
D000241:Adenosine / Q000031:analogs & derivatives* / Q000138:chemical synthesis; D008954:Models, Biological; D014176:Protein Biosynthesis; D012346:RNA, Transfer, Amino Acyl / Q000378:metabolism*; D012270:Ribosomes / Q000378:metabolism*
D012346:RNA, Transfer, Amino Acyl; C019037:tRNA, peptidyl-; D000241:Adenosine