Immune responses of Asian sea bass, Lates calcarifer  Bloch, against an inactivated betanodavirus vaccine.
Asian sea bass, Lates calcarifer (Bloch), exhibited strong immune responses against a single injection of the formalin-inactivated red-spotted grouper nervous necrosis virus (RGNNV), a betanodavirus originally isolated in Japan. Fish produced neutralizing antibodies at high titre levels from days 10 (mean titre 1:480) to 116 (1:1280), with the highest titre at day 60 post-vaccination (1:4480). When fish were challenged with the homologous RGNNV at day 54 post-vaccination, there were no mortalities in both the vaccinated and unvaccinated control fish. However, a rapid clearance of the virus was observed in the brains and kidneys of vaccinated fish, followed by a significant increase in neutralizing-antibody titres. Furthermore, the vaccine-induced antibodies potently neutralized Philippine betanodavirus isolates (RGNNV) in a cross-neutralization assay. The present results indicate the potential of the formalin-inactivated RGNNV vaccine against viral nervous necrosis (VNN) of Asian seabass.
Pakingking
R
R
Southeast Asian Fisheries Development Center, Iloilo, Philippines. rpakingking@seafdec.org.ph
Seron
R
R
dela Peña
L
L
Mori
K
K
Yamashita
H
H
Nakai
T
T
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D001492:Bass / Q000276:immunology*; D009500:Neutralization Tests; D030381:Nodaviridae / Q000276:immunology*; D015164:Vaccines, Inactivated / Q000276:immunology; D014765:Viral Vaccines / Q000276:immunology*
D000914:Antibodies, Viral; D015164:Vaccines, Inactivated; D014765:Viral Vaccines