Effects of high fat diet on Morris maze performance, oxidative stress, and inflammation in rats: contributions of maternal diet.
This study was undertaken to investigate the effects of prenatal and postnatal exposure to high fat diet on the brain. Female rats were divided into high fat diet (HFD) and control diet (CD) groups 4 weeks prior to breeding and throughout gestation and lactation. After weaning, male progeny were placed on a chow diet until 8 weeks old, and then segregated into HFD or CD groups. At 20 weeks old, rats were evaluated in the Morris water maze, and markers of oxidative stress and inflammation were documented in the brain. In comparison to rats fed CD, cognitive decline in HFD progeny from HFD dams manifested as a decline in retention, but not acquisition, in the water maze. HFD was also associated with significant increases in 3-nitrotyrosine, inducible nitric oxide synthase, IL-6, and glial markers Iba-1 and GFAP, with the largest increases frequently observed in HFD animals born to HFD dams. Thus, these data collectively suggest that HFD increases oxidative and inflammatory signaling in the brain, and further indicate that maternal HFD consumption might sensitize offspring to the detrimental effects of HFD.
White
Christy L
CL
Pennington Biomedical Research Center, Louisiana State University System, Baton Rouge, LA 70808, USA.
Pistell
Paul J
PJ
Purpera
Megan N
MN
Gupta
Sunita
S
Fernandez-Kim
Sun-Ok
SO
Hise
Taylor L
TL
Keller
Jeffrey N
JN
Ingram
Donald K
DK
Morrison
Christopher D
CD
Bruce-Keller
Annadora J
AJ
D000704:Analysis of Variance; D000818:Animals; D000831:Animals, Newborn; D001823:Body Composition; D001835:Body Weight; D001921:Brain / Q000378:metabolism / Q000503:physiopathology; D002135:Calcium-Binding Proteins / Q000378:metabolism; D016207:Cytokines / Q000378:metabolism; D004041:Dietary Fats / Q000494:pharmacology*; D004195:Disease Models, Animal; D004622:Embryo, Mammalian; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005260:Female; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects / Q000502:physiology; D005904:Glial Fibrillary Acidic Protein; D007249:Inflammation / Q000209:etiology / Q000378:metabolism* / Q000473:pathology; D007774:Lactation / Q000187:drug effects / Q000502:physiology; D008297:Male; D018782:Maze Learning / Q000187:drug effects / Q000502:physiology*; D008840:Microfilament Proteins; D009419:Nerve Tissue Proteins / Q000378:metabolism; D052247:Nitric Oxide Synthase Type II / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects / Q000502:physiology*; D011247:Pregnancy; D011297:Prenatal Exposure Delayed Effects / Q000503:physiopathology*; D039401:Prenatal Nutritional Physiological Phenomena; D051381:Rats; D020318:Rats, Long-Evans
C491049:Aif1 protein, mouse; D002135:Calcium-Binding Proteins; D016207:Cytokines; D004041:Dietary Fats; D005904:Glial Fibrillary Acidic Protein; D008840:Microfilament Proteins; D009419:Nerve Tissue Proteins; C528900:glial fibrillary astrocytic protein, mouse; D052247:Nitric Oxide Synthase Type II