Proliferation of fibroblasts cultured on a hemi-cellulose dressing.
In recent times, hemi-cellulose dressing (HD) has been used clinically with satisfactory rates of success [Melandri D, De Angelis A, Orioli R, et-al. Use of a new hemicellulose dressing (Veloderm) for the treatment of split-thickness skin graft donor sites A within-patient controlled study. Burns 2006 Dec;32:964-72.]; however, the effect of cellulose dressings on the wound-healing process is unclear due to paucity of experimental data. This study aimed to determine the adhesion and proliferation of human skin fibroblasts, which were cultured in vitro using the explant technique, on HD. Cells were seeded onto HD discs and evaluated for cell adhesion and cell proliferation after 7, 14 and 21 days. Fibroblasts displayed 70% adhesion to HD after 24h. The HD discs seeded with a density of 5x10(4) cells per well showed a proliferation rate of 12% on day 7, 30% on day 14 and 75% on day 21. The results demonstrated that HD can sustain fibroblast proliferation--a highly desirable characteristic for an ideal skin substitute.
Ferreira
Lydia M
LM
Universidade Federal de São Paulo, São Paulo, Brazil. lydia.dcir@epm.br
Sobral
Christiane S
CS
Blanes
Leila
L
Ipolito
Michelle Z
MZ
Horibe
Elaine K
EK
D001458:Bandages; D002448:Cell Adhesion / Q000187:drug effects; D049109:Cell Proliferation; D002478:Cells, Cultured; D002648:Child; D002675:Child, Preschool; D005347:Fibroblasts / Q000166:cytology*; D006801:Humans; D011134:Polysaccharides / Q000494:pharmacology*; D012867:Skin / Q000166:cytology* / Q000254:growth & development; D014945:Wound Healing / Q000187:drug effects*; D014947:Wounds and Injuries / Q000628:therapy
D011134:Polysaccharides; C007916:hemicellulose