Associations of a HinfI PCR-RFLP of POU1F1 gene with growth traits in Qinchuan cattle.
The objectives of the present study were to estimate the allele and genotype frequencies of the POU1F1/HinfI polymorphisms in beef cattle belonging to four different genetic groups and to determine the effects of these polymorphisms on growth traits in cattle. The 451-bp PCR products of POU1F1 gene digested with HinfI exhibited three genotypes and two alleles, which were at Hardy-Weinberg equilibrium (P > 0.05). Genotype BB was the predominant genotype and B the predominant allele in the studied populations. There was significant difference between Limousin x Qinchuan and Qinchuan in the distribution of genotypes (P < 0.0001). The association of the polymorphism of the POU1F1 gene with growth traits among Qinchuan, Limousin x Qinchuan, Angus x Qinchuan and Germany Yellow x Qinchuan crosses was analyzed. Body weight and wither height of individuals with genotypes AB were higher (P < 0.05)than that of individuals with genotype BB in the Germany Yellow x Qinchua cross, but were not in the other three populations (P > 0.05).
Zhang
Chunlei
C
Northwest A&F University, Yangling, Shaaxi, P.R. China.
Liu
Bo
B
Chen
Hong
H
Lan
Xianyong
X
Lei
Chuzhao
C
Zhang
Zhiqing
Z
Zhang
Runfeng
R
D000483:Alleles; D000818:Animals; D001483:Base Sequence; D049628:Body Size / Q000235:genetics; D001835:Body Weight / Q000235:genetics; D001947:Breeding; D002417:Cattle / Q000145:classification / Q000235:genetics* / Q000254:growth & development*; D017931:DNA Primers / Q000235:genetics; D015252:Deoxyribonucleases, Type II Site-Specific; D005260:Female; D005787:Gene Frequency; D006824:Hybridization, Genetic; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D013045:Species Specificity; D050817:Transcription Factor Pit-1 / Q000235:genetics*
D017931:DNA Primers; D050817:Transcription Factor Pit-1; D015252:Deoxyribonucleases, Type II Site-Specific; C032229:GANTC-specific type II deoxyribonucleases