The drs tumor suppressor is involved in the maturation process of autophagy induced by low serum.
The drs gene is an apoptosis-inducing tumor suppressor. Previously, we showed that drs contributes to the suppression of tumor formation by generating drs-knockout mice. In this study, by using drs KO mouse embryonic fibroblasts, we found that drs is involved in the autophagy regulation under low serum culture conditions. Both electron microscopy and GFP-LC3 analyses demonstrated that drs is involved in the maturation process of autophagy from autophagosomes to autolysosomes. In addition, drs could associate with Rab24 and the association between drs and Rab24 was enhanced during autophagy. Drs was also co-localized with Rab24 on punctuated structures during autophagy.
Tambe
Yukihiro
Y
Division of Microbiology and Infectious Diseases, Department of Pathology, Shiga University of Medical Science, Setatsukinowa-cho, Otsu, Shiga 520-2192, Japan.
Yamamoto
Akitsugu
A
Isono
Takahiro
T
Chano
Tokuhiro
T
Fukuda
Mitsunori
M
Inoue
Hirokazu
H
D000818:Animals; D001343:Autophagy / Q000502:physiology*; D018929:Cell Culture Techniques; D002478:Cells, Cultured; D016172:DNA Fingerprinting; D005347:Fibroblasts / Q000378:metabolism*; D005455:Fluorescent Antibody Technique; D016147:Genes, Tumor Suppressor / Q000502:physiology*; D006801:Humans; D015151:Immunoblotting; D047468:Immunoprecipitation; D008297:Male; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D018345:Mice, Knockout; D046529:Microscopy, Electron, Transmission; D010588:Phagosomes / Q000235:genetics / Q000378:metabolism*; D044967:Serum; D020691:rab GTP-Binding Proteins / Q000378:metabolism
D008565:Membrane Proteins; C486298:Srpx protein, mouse; C086224:Rab24 protein, mouse; D020691:rab GTP-Binding Proteins