Rapid sequencing gel electrophoresis using glycerol-tolerant sodium taurine medium.
We describe a new glycerol-tolerant sodium taurine (ST) medium for rapid sequencing gel electrophoresis by substituting the standard conductive media of Tris-boric acid-ethylenediaminetetraacetic acid (EDTA) (TBE) and Tris-taurine-EDTA (TTE) and other low-ionic-strength media of sodium boric acid (SB). Low-ionic-strength and cost-effective ST media gave glycerol tolerance up to 50% (v/v) glycerol-containing DNA sample solution, shorter running time, and better resolution to separate small DNA oligonucleotides (20-45 mer) in 12% denaturing sequencing gel electrophoresis.
Health Technology Research Center, National Institute of Advanced Industrial Science and Technology, Takamatsu, Kagawa 761-0395, Japan. hirano-ken@aist.go.jp
Ken
K
Hirano
Tomomi
T
Ishido
Mitsuru
M
Ishikawa
D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D005990:Glycerol / Q000737:chemistry*; D009841:Oligonucleotides / Q000032:analysis; D017422:Sequence Analysis, DNA / Q000379:methods*; D013654:Taurine / Q000737:chemistry*
D009841:Oligonucleotides; D013654:Taurine; D005990:Glycerol