A primer for morpholino use in zebrafish.
Morpholino oligonucleotides are the most common anti-sense "knockdown" technique used in zebrafish (Danio rerio). This review discusses common practices for the design, preparation, and deployment of morpholinos in this vertebrate model system. Off-targeting effects of morpholinos are discussed as well as method to minimize this potentially confounding variable via co-injection of a tP53-targeting morpholino. Finally, new uses of morpholinos are summarized and contextualized with respect to the complementary, DNA-based knockout technologies recently developed for zebrafish.
Bill
Brent R
BR
Department of Genetics, Cell Biology, and Development, University of Minnesota, Minneapolis, Minnesota, USA.
Petzold
Andrew M
AM
Clark
Karl J
KJ
Schimmenti
Lisa A
LA
Ekker
Stephen C
SC
D000818:Animals; D055785:Gene Knockdown Techniques; D009841:Oligonucleotides / Q000235:genetics*; D015027:Zebrafish / Q000235:genetics*
D009841:Oligonucleotides