Analysis of the bactericidal effect of ozone pneumoperitoneum.
OBJECTIVE
To assess the bactericidal action of ozone pneumoperitonium, and to compare the results with CO2.


METHODS
It was used 36 Wistar rats. The animals, under anesthesia, were inoculated with 2ml of E. coli ATCC at a concentration of 10(10)UFC, and 1ml of BaSO4, into the peritoneal cavity. They were divided into three groups: Group 1, CO2 pneumoperitoneum was performed for 15 minutes; Group 2, ozone pneumoperitoneum was performed for 5 minutes at a concentration of 42microg/ml, and Group 3, ozone pneumoperitoneum was performed for 5 minutes at a concentration of 62microg/ml. Six animals from each group were sacrificed after the experiment, and the remaining 6 observed for 24 hours. Material was collected from the cavity of all animals for microbiological study.


RESULTS
Ozone presented a greater bactericidal effect than CO2 in those animals sacrificed immediately after pneumoperitoneum. In the animals studied 24 hours after pneumoperitoneum evidenced no difference in bactericidal effect between the two gases. Moreover, no difference in mortality was observed.


CONCLUSIONS
Ozone has a more potent bactericidal effect than carbon dioxide gas, although this did not influence survival of the animals.
Santa Casa de Sao Paulo, Faculty of Medical Sciences, Sao Paulo, Brazil. roaltenfelder@terra.com.br
Rodrigo Altenfelder
RA
Silva
José Eduardo Rosseto
JE
Garotti
Renata Santos Bittencourt
RS
Silva
Alessandra
A
Navarini
Adhemar Monteiro
AM
Pacheco
D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002245:Carbon Dioxide / Q000494:pharmacology; D004926:Escherichia coli / Q000187:drug effects*; D010126:Ozone / Q000494:pharmacology*; D010538:Peritonitis / Q000188:drug therapy / Q000382:microbiology*; D011027:Pneumoperitoneum / Q000188:drug therapy / Q000382:microbiology*; D051381:Rats; D017208:Rats, Wistar; D018709:Statistics, Nonparametric
D000900:Anti-Bacterial Agents; D002245:Carbon Dioxide; D010126:Ozone