Four novel resistance integron gene-cassette occurrences in bacterial isolates from zhenjiang, china.
Integrons, which are widely distributed among bacteria and are strongly associated with resistance, are specialized genetic elements that are capable of capturing, integrating, and mobilizing gene cassette. In this work, we investigated classes 1, 2, and 3 integrons associated integrases genes in 365 bacteria isolates, amplified and analyzed the structure of class 1 integron, detected 8 resistant gene cassettes [dfr17, aadA5, aadA1, aadA2, dhfrI, aadB, aac(6')-II, and pse-I], and found four novel gene-cassette arrays. We also found that commensal bacteria in the common microenvironment had the same integron gene cassette, which provided direct evidence that integron was an important horizontal transmission element.
Xu
Huaxi
H
Department of Microbiology and Immunology, Institute of Clinical Laboratory Medicine, Jiangsu University, Zhenjiang, 212013, China. xuhx@ujs.edu.cn
Su
Zhaoliang
Z
Wang
Shengjun
S
Dai
Xiaoli
X
Chen
Jianguo
J
Kong
Fanzhi
F
Li
Yazhen
Y
Peng
Sufang
S
Shao
Qixiang
Q
Lu
Liwei
L
Ezaki
T
T
D001419:Bacteria / Q000187:drug effects* / Q000235:genetics* / Q000302:isolation & purification; D001424:Bacterial Infections / Q000382:microbiology; D002681:China; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D024881:Drug Resistance, Bacterial; D004783:Environmental Microbiology; D023061:Gene Order; D005798:Genes, Bacterial; D006801:Humans; D032023:Integrons; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA
D004269:DNA, Bacterial