Transience of MHC Class I-restricted antigen presentation after influenza A virus infection.
Antigen expressed as MHC Class I glycoprotein (pMHCI) complexes on dendritic cells is the primary driver of CD8(+) T cell clonal expansion and differentiation. As we seek to define the molecular differences between acutely stimulated cytotoxic T lymphocyte (CTL) effectors and long-lived memory T cells, it is essential that we understand the duration of in vivo pMHCI persistence. Although infectious influenza A virus is readily cleared by mammalian hosts, that does not necessarily mean that all influenza antigen is totally eliminated. An exhaustive series of carefully controlled adoptive transfer experiments using 3 different carboxy fluorescein diacetate succinimidyl ester-labeled T cell receptor-transgenic CTL populations and a spectrum of genetically engineered and wild-type influenza A viruses provided no evidence for pMHCI persistence over the 30-60-d interval after virus challenge. Molecular profiles identified in antigen-specific T cells at this time may thus be considered to reflect established immunologic memory and not recent CTL activation from a persistent pMHCI pool.
Department of Microbiology and Immunology, University of Melbourne, Parkville 3010, Australia.
Justine D
JD
Mintern
Sammy
S
Bedoui
Gayle M
GM
Davey
Jessica M
JM
Moffat
Peter C
PC
Doherty
Stephen J
SJ
Turner
D000818:Animals; D017951:Antigen Presentation / Q000276:immunology*; D039521:CD11c Antigen / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D002465:Cell Movement; D003713:Dendritic Cells / Q000276:immunology; D000939:Epitopes / Q000276:immunology; D005260:Female; D005452:Fluoresceins; D015395:Histocompatibility Antigens Class I / Q000276:immunology*; D007249:Inflammation / Q000150:complications / Q000276:immunology; D009980:Influenza A virus / Q000276:immunology*; D007700:Kinetics; D008168:Lung / Q000276:immunology / Q000821:virology; D008213:Lymphocyte Activation / Q000276:immunology; D018655:Lymphocyte Count; D051379:Mice; D008810:Mice, Inbred C57BL; D009976:Orthomyxoviridae Infections / Q000150:complications / Q000276:immunology*; D010047:Ovalbumin / Q000276:immunology; D010641:Phenotype; D013388:Succinimides; D013602:T-Lymphocytes, Cytotoxic / Q000166:cytology / Q000276:immunology; D013997:Time Factors
C087165:5-(6)-carboxyfluorescein diacetate succinimidyl ester; D039521:CD11c Antigen; D000939:Epitopes; D005452:Fluoresceins; D015395:Histocompatibility Antigens Class I; D013388:Succinimides; D010047:Ovalbumin