Non-invasive monitoring of redox status in mice with dextran sodium sulphate-induced colitis.
Change of redox status is associated with colitis induced by dextran sodium sulphate (DSS). This study monitored redox status in DSS-induced colitis in mice using in vivo electron spin resonance (ESR) spectroscopy with nitroxyl probes. Colitis was induced in male ICR mice by supplementing their drinking water with 3% DSS for 3, 5 or 7 days. The ESR signal decay rate of carbamoyl-PROXYL administered into the rectum was enhanced by DSS treatment and the enhancement on day 7 was suppressed by membrane-permeable antioxidants, tiron and dimethylsulphoxide and a membrane-impermeable antioxidant, mannitol. The enhancement on day 5 was suppressed by tiron and dimethylsulphoxide, while that on day 3 was inhibited only by tiron. These results suggest that redox change occurs in or around of epithelial cells on day 7, but only intracellularly on day 5, and that redox change such as generation of less reactive radicals occurs only intracellularly on day 3.
Department of Bio-functional Science, Faculty of Pharmaceutical Sciences, Kyushu University, Fukuoka, Japan.
Keiji
K
Yasukawa
Reiko
R
Miyakawa
Takashi
T
Yao
Masazumi
M
Tsuneyoshi
Hideo
H
Utsumi
D014013:1,2-Dihydroxybenzene-3,5-Disulfonic Acid Disodium Salt / Q000494:pharmacology; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D003093:Colitis, Ulcerative / Q000139:chemically induced* / Q000378:metabolism* / Q000473:pathology; D016264:Dextran Sulfate / Q000633:toxicity*; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D004195:Disease Models, Animal; D004578:Electron Spin Resonance Spectroscopy; D007413:Intestinal Mucosa / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D008297:Male; D008353:Mannitol / Q000494:pharmacology; D051379:Mice; D008813:Mice, Inbred ICR; D010084:Oxidation-Reduction; D011759:Pyrrolidines; D013113:Spin Labels; D013997:Time Factors
C538936:2,2,5,5,-tetramethylpyrrolidine-1-oxyl; D000975:Antioxidants; D011759:Pyrrolidines; D013113:Spin Labels; D008353:Mannitol; D014013:1,2-Dihydroxybenzene-3,5-Disulfonic Acid Disodium Salt; D016264:Dextran Sulfate; D004121:Dimethyl Sulfoxide