[Toxicity of nano silica particles on rat type I-like alveolar epithelial cells].
OBJECTIVE
To access rat lung toxicity of nano silica particles.


METHODS
Transmission electron microscope was used to observe size, shape and dispersibility of two silica particles; Size of two particles in water and RPMI 1640 containing 1% FBS were measured using Zeta Potential Analyzer. LDH activities of rat type I-like alveolar epithelial cell line R3/1 cells after 6, 24 and 48 h incubation with 2.5, 5.0, 10.0, 20.0 microg/ml of two silica nano particles were detected by spectrophotometric method; protein carbonylation and MIP-2 release of R3/1 cells after 24 h incubation with 2.5, 5.0, 10.0, 20.0 microg/ml of two silica nano particles were measured using ELISA kits.


RESULTS
TEM image showed nano silica particles were round and dispersed evenly; the average sizes of the two silica particles were (43+/-4.2) and (68+/-5.7) nm. Two silica particles had similar size in water and RPMI 1640 containing 1% FBS, respectively. Both nano silica particles in 2.5 approximately 20.0 microg/ml dose range did not cause significant increase of LDH activities (P>0.05), did not elevate protein carbonylation and MIP-2 levels in R3/1 cells (P>0.05).


CONCLUSIONS
Two nano silica particles do not have lung toxicity in 2.5 approximately 20.0 microg/ml dose range.
Department of Hygienic Toxicology, School of Public Health, Jilin University, Changchun 130021, China.
Wen
W
Wang
Günter
G
Oberdörster
Xiao-mei
XM
Liu
Lei
L
Sun
Hai-ying
HY
Du
Long
L
Zhang
Wen-sheng
WS
Yang
Zhi-wei
ZW
Sun
D056809:Alveolar Epithelial Cells / Q000187:drug effects* / Q000378:metabolism; D000818:Animals; D002478:Cells, Cultured; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D053758:Nanoparticles / Q000633:toxicity*; D051381:Rats; D012822:Silicon Dioxide / Q000633:toxicity*; D018675:Toxicity Tests
D012822:Silicon Dioxide; D007770:L-Lactate Dehydrogenase