Metabolite interference in pharmacokinetic studies.
Our policy of conducting biotransformation studies with extended chromatography prior to pharmacokinetic bioanalyses allowed us to quickly detect an unusual, cis/trans metabolite in rat plasma that was inseparable using a short chromatographic method. We caution investigators that short methods invite unknown isobaric metabolites to cause inaccuracies in plasma concentration measurements.
Moghaddam
Mehran F
MF
Celgene, 4550 Towne Centre Ct, San Diego, CA 92121, USA.
Fan
Jui-Hsiang
JH
Tang
Yang
Y
Khatsenko
Oleg
O
Katz
Jason C
JC
Shirley
Michael A
MA
D000818:Animals; D001711:Biotransformation; D002853:Chromatography, Liquid / Q000379:methods*; D003511:Cyclohexanols / Q000378:metabolism*; D055808:Drug Discovery / Q000379:methods; D005260:Female; D022781:Hepatocytes / Q000378:metabolism; D008297:Male; D004364:Pharmaceutical Preparations / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013237:Stereoisomerism
D003511:Cyclohexanols; D004364:Pharmaceutical Preparations