Polymorphisms of interleukin-6 and tumor necrosis factor gene promoters and cardiorespiratory function following liver transplantation: a preliminary study.
BACKGROUND
The interindividual variability in cardiorespiratory function during liver transplantation (OLT) has been attributed to various factors, including polymorphisms in immunity genes known to affect the circulation levels of cytokines.


OBJECTIVE
To evaluate polymorphisms of genes encoding for interleukin-6 (IL6) and tumor necrosis factor (TNF) in association with cardiorespiratory function in OLT.


METHODS
Prospective observational study.


METHODS
We studied 62 consecutive patients who had OLT performed in our hospital between 2004 and 2005. Polymorphisms at positions -308 and -409 of TNF gene, as well as those at -174 and -574 of IL6 gene were determined in all patients by means of PCR-RFLPs. Associations were carried out using chi-square tests and analysis of variance. A bilateral P < .05 was accepted as significant.


RESULTS
No statistically significant associations were observed.


CONCLUSIONS
A relationship between the polymorphisms studied and respiratory function in OLT was lacking. These results must be interpreted with caution due to the limited sample size.
Arranz Duran
J
J
Liver Transplant Unit, Hospital Universitario NS de Candelaria, Tenerife, Spain. javierarranz@telefonica.net
Arteaga González
A
A
Domínguez García
D
D
Calvo Falcón
R
R
Flores
C
C
González-Paredes
F J
FJ
Sanz Pereda
P
P
Méndez Alvárez
S
S
García Cobaleda
I
I
Aguirre Jaime
A
A
Arteaga González
I
I
Varona Bosque
A
A
Barrera Gómez
M
M
Soriano Benítez de Lugo
A
A
D000704:Analysis of Variance; D016009:Chi-Square Distribution; D006334:Heart Function Tests; D006801:Humans; D015850:Interleukin-6 / Q000235:genetics*; D007902:Length of Stay; D016031:Liver Transplantation / Q000502:physiology*; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D011401:Promoter Regions, Genetic; D012129:Respiratory Function Tests; D012189:Retrospective Studies; D016896:Treatment Outcome; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics*
D015850:Interleukin-6; D014409:Tumor Necrosis Factor-alpha