Loss of vancomycin bactericidal activity against accessory gene regulator (agr) dysfunctional Staphylococcus aureus under conditions of high bacterial density.
The impact of accessory gene regulator (agr) dysfunction and high bacterial density on vancomycin killing and resistance was evaluated among 10 clinical methicillin-resistant Staphylococcus aureus bloodstream isolates using time kill experiments. Under conditions of high inocula and agr dysfunction, vancomycin activity was markedly attenuated, amplifying resistant mutants by 72 h.
Tsuji
Brian T
BT
The New York State Center of Excellence in Bioinformatics & Life Sciences, University at Buffalo, New York 14205, USA. btsuji@buffalo.edu
Harigaya
Yoriko
Y
Lesse
Alan J
AJ
Sakoulas
George
G
Mylotte
Joseph M
JM
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D016470:Bacteremia / Q000382:microbiology; D001426:Bacterial Proteins / Q000378:metabolism*; D015169:Colony Count, Microbial; D006801:Humans; D055624:Methicillin-Resistant Staphylococcus aureus / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification; D050296:Microbial Viability / Q000187:drug effects*; D009154:Mutation; D013203:Staphylococcal Infections / Q000382:microbiology; D013997:Time Factors; D015534:Trans-Activators / Q000378:metabolism*; D014640:Vancomycin / Q000494:pharmacology*; D020713:Vancomycin Resistance
C095698:Agr protein, Staphylococcus aureus; D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D015534:Trans-Activators; D014640:Vancomycin