Responses of plasma acetate metabolism to hop (Humulus lupulus L.) in sheep.
An isotope dilution method using [1-(13)C]sodium (Na) acetate was conducted to determine the effect of feeding hop (Humulus lupulus L.) residues on plasma acetate metabolism in six adult crossbred sheep. The sheep were fed 63 g/kg BW(0.75)/d of either mixed hay (MH-diet) of orchardgrass (Dactylis glomerata L.) and reed canarygrass (Phalaris arundinacea L.) at a 60:40 ratio or MH-diet and hop-residues (Hop-diet) at 85:15 ratio with a crossover design for each of 3 week period. The isotope dilution method using single injection of [1-(13)C]Na acetate was performed thrice; before feeding (BF), 2 h after feeding (2F) and 4 h after feeding (4F), on the 21(st) day of each dietary treatment. Plasma acetate concentration tended to increase (P=0.06) and turnover rate was numerically higher (P=0.16) for MH-diet than Hop-diet. Plasma glucose, NEFA, VFA and lactic acid concentrations were similar between dietary treatments. In both the diets, although plasma concentration of acetate did not change, turnover rate increased significantly (P=0.02) 2F than BF. Hop-residues did not show any negative impacts on acetate metabolism as well as physiology of animals in the present experimental conditions, hence thereby it could be used as an alternative to MH-diet for rearing sheep.
Department of Animal Science, Faculty of Agriculture, Iwate University, Morioka 020-8550, Japan.
Mohammad
M
Al-Mamun
Kunio
K
Goto
Sota
S
Chiba
Hiroaki
H
Sano
D000085:Acetates / Q000097:blood*; D000821:Animal Feed; D000818:Animals; D001786:Blood Glucose / Q000032:analysis; D002247:Carbon Isotopes; D004032:Diet; D005230:Fatty Acids, Nonesterified / Q000097:blood; D005232:Fatty Acids, Volatile / Q000097:blood / Q000378:metabolism; D005260:Female; D027582:Humulus; D007201:Indicator Dilution Techniques; D019344:Lactic Acid / Q000097:blood; D008297:Male; D017672:Nitrogen Compounds / Q000378:metabolism; D012417:Rumen / Q000502:physiology; D012756:Sheep / Q000378:metabolism*; D019346:Sodium Acetate
D000085:Acetates; D001786:Blood Glucose; D002247:Carbon Isotopes; D005230:Fatty Acids, Nonesterified; D005232:Fatty Acids, Volatile; D017672:Nitrogen Compounds; D019344:Lactic Acid; D019346:Sodium Acetate