Modulation of porcine (Sus scrofa domestica) and pheasant (Phasianus colchicus) carbonyl reducing enzymes by anthelmintic therapy with flubendazole.
Flubendazole (FLU) is a widely administered benzimidazole anthelmintic indicated for the control of parasitic diseases in farm animals including pigs and pheasants. This study was designed to test the biotransformation of FLU in control animals and animals treated with FLU in recommended therapeutic doses. The activities of several pheasant and porcine hepatic and intestinal carbonyl reducing enzymes and their modulation by FLU were also studied. Twelve adult pheasant hens, approximately 1 year old, were divided into two groups and treated for 7 days with placebo or 6 mg of FLU/kg of body weight. Eight male hog weaners, approximately 3 month old, were divided into two groups and treated for 5 days with placebo or 1.57 mg of FLU/kg of body weight. Subcellular fractions, prepared from livers and small intestines of control and FLU treated animals, were incubated with FLU. In vitro formation of two main FLU metabolites, reduced FLU, and hydrolyzed FLU were analyzed using HPLC. While FLU was reduced significantly more intensively in FLU-treated pheasants than in control animals, no differences were observed in pigs. These results were confirmed by measuring the enzyme activities: carbonyl reducing enzyme activities were increased in pheasants treated by FLU, whereas FLU did not affect these enzymes in pigs.
Department of Biochemical Sciences, Faculty of Pharmacy, Charles University, Heyrovského 1203, CZ-50005 Hradec Králové, Czech Republic. Barbora.Szotakova@faf.cuni.cz
Barbora
B
Szotáková
Milan
M
Nobilis
Jirí
J
Lamka
Veronika
V
Krízová
Michal
M
Savlík
Lenka
L
Skálová
D000429:Alcohol Oxidoreductases / Q000187:drug effects* / Q000378:metabolism; D000818:Animals; D000969:Antinematodal Agents / Q000378:metabolism / Q000494:pharmacology*; D002851:Chromatography, High Pressure Liquid; D005260:Female; D046094:Galliformes; D007422:Intestines / Q000187:drug effects / Q000201:enzymology; D008099:Liver / Q000187:drug effects / Q000201:enzymology; D008297:Male; D008463:Mebendazole / Q000031:analogs & derivatives* / Q000378:metabolism / Q000494:pharmacology; D010273:Parasitic Diseases, Animal / Q000188:drug therapy; D013045:Species Specificity; D034421:Sus scrofa
D000969:Antinematodal Agents; D008463:Mebendazole; D000429:Alcohol Oxidoreductases; C018945:flubendazole