Expression of growth differentiation factor-9 and bone morphogenetic protein-15 in oocytes and cumulus granulosa cells of patients with polycystic ovary syndrome.
OBJECTIVE
To evaluate the effect of growth differentiation factor-9 (GDF-9) and bone morphogenetic protein-15 (BMP-15) on the development of follicles among patients with polycystic ovary syndrome (PCOS).


METHODS
Case-control study.


METHODS
University Hospital.


METHODS
Twenty-two oocytes were obtained from 15 patients with PCOS and 67 oocytes from 58 controls. Cumulus granulosa cells (GC) were obtained from 16 patients with PCOS and controls treated with intracytoplasmic sperm injection.


METHODS
Immunofluorescence combined with laser scanning confocal microscopy and immunocytochemistry were used to analyze the expression of GDF-9 and BMP-15 in oocytes and cumulus GCs.


METHODS
To detect the protein expression levels.


RESULTS
No significant difference was found in the expression of GDF-9 and BMP-15 in the oocytes and BMP-15 in the cumulus GCs of patients with PCOS and controls. However, the expression of GDF-9 in cumulus GCs of patients with PCOS was decreased significantly compared with controls (8.88 +/- 1.52 vs. 5.01 +/- 0.83).


CONCLUSIONS
The expression of GDF-9 and BMP-15 in the oocytes of patients with PCOS who received ovulation induction treatment was in the normal range, but the GDF-9 expression in cumulus GCs from patients with PCOS was significantly lower than the normal. Reduced GDF-9 expression in cumulus GCs of patients with PCOS appears to be associated with decreased long-term developmental potential of the oocytes of patients with PCOS.
Department of Obstetrics and Gynecology, Peking University Third Hospital, Beijing, People's Republic of China.
Shu-Yun
SY
Zhao
Jie
J
Qiao
Yong-Jian
YJ
Chen
Ping
P
Liu
Jian
J
Li
Jie
J
Yan
D000328:Adult; D055430:Bone Morphogenetic Protein 15 / Q000096:biosynthesis* / Q000235:genetics; D016022:Case-Control Studies; D054885:Cumulus Cells / Q000378:metabolism* / Q000473:pathology; D005260:Female; D005786:Gene Expression Regulation; D055429:Growth Differentiation Factor 9 / Q000037:antagonists & inhibitors / Q000096:biosynthesis* / Q000235:genetics; D006801:Humans; D009865:Oocytes / Q000378:metabolism*; D011085:Polycystic Ovary Syndrome / Q000378:metabolism* / Q000473:pathology; D020554:Sperm Injections, Intracytoplasmic / Q000379:methods
D055430:Bone Morphogenetic Protein 15; D055429:Growth Differentiation Factor 9