Sauvatide--a novel amidated myotropic decapeptide from the skin secretion of the waxy monkey frog, Phyllomedusa sauvagei.
Here we describe the structural and functional characterization of a novel myotropic peptide, sauvatide, from the skin secretion of the waxy monkey frog, Phyllomedusa sauvagei. Sauvatide is a C-terminally amidated decapeptide with the following primary structure--LRPAILVRTKamide--monoisotopic mass 1164.77Da, which was found to contract the smooth muscle of rat urinary bladder with an EC(50) of 2.2 nM. The sauvatide precursor, deduced from cloned skin cDNA, consists of 62 amino acid residues with a single copy of sauvatide located near the C-terminus. The mature peptide is generated from the precursor by cleavage at a classical -KR-cleavage site located proximal to the N-terminus and by removal of a -GKGK sequence at the C-terminus, the first glycyl residue acting as amide donor. Amphibian skin secretions thus continue to be a source of novel and potent biologically active peptides acting through functional targets in mammalian tissues.
Wang
Lei
L
Molecular Therapeutics Research, School of Pharmacy, Queen's University, Medical Biology Centre, Belfast, Northern Ireland, UK.
Zhou
Mei
M
Zhou
Zhihao
Z
Chen
Tianbao
T
Walker
Brian
B
Shaw
Chris
C
D000595:Amino Acid Sequence; D000818:Animals; D001001:Anura / Q000378:metabolism*; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D008969:Molecular Sequence Data; D009119:Muscle Contraction; D009130:Muscle, Smooth / Q000187:drug effects* / Q000502:physiology; D009842:Oligopeptides / Q000737:chemistry / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology*; D051381:Rats; D012867:Skin / Q000378:metabolism*; D001743:Urinary Bladder / Q000187:drug effects / Q000502:physiology
D018076:DNA, Complementary; D009842:Oligopeptides; C540676:leucyl-arginyl-prolyl-alanyl-isoleucyl-leucyl-valyl-arginyl-threonyl-lysinamide