The promotion of human mesenchymal stem cell proliferation by superparamagnetic iron oxide nanoparticles.
Superparamagnetic iron oxide (SPIO) nanoparticles are very useful in cell imaging; meanwhile, however, biosafety concerns associated with their use, especially on therapeutic stem cells, have arisen. Most studies of biosafety issues focus on whether the nanoparticles have deleterious effects. Here, we report that Ferucarbotran, an ionic SPIO, is not toxic to human mesenchymal stem cells (hMSCs) under the conditions of these experiments but instead increases cell growth. Ferucarbotran-promoted cell growth is due to its ability to diminish intracellular H2O2 through intrinsic peroxidase-like activity. Also, Ferucarbotran can accelerate cell cycle progression, which may be mediated by the free iron (Fe) released from lysosomal degradation and involves the alteration of Fe on the expression of the protein regulators of the cell cycle.
Center for Nanomedicine Research, National Health Research Institutes, 35 Keyan Road, Zhunan, Miaoli 350, Taiwan. dmhuang@nhri.org.tw
Dong-Ming
DM
Huang
Jong-Kai
JK
Hsiao
Ying-Chun
YC
Chen
Li-Ying
LY
Chien
Ming
M
Yao
Yin-Kai
YK
Chen
Bor-Sheng
BS
Ko
Szu-Chun
SC
Hsu
Lin-Ai
LA
Tai
Hui-Ying
HY
Cheng
Shih-Wei
SW
Wang
Chung-Shi
CS
Yang
Yao-Chang
YC
Chen
D000818:Animals; D002453:Cell Cycle / Q000502:physiology; D049109:Cell Proliferation / Q000187:drug effects*; D003287:Contrast Media / Q000737:chemistry / Q000494:pharmacology; D003911:Dextrans; D005290:Ferric Compounds / Q000737:chemistry / Q000494:pharmacology*; D052203:Ferrosoferric Oxide; D006801:Humans; D006861:Hydrogen Peroxide / Q000378:metabolism; D007501:Iron / Q000737:chemistry / Q000494:pharmacology*; D008279:Magnetic Resonance Imaging; D008280:Magnetics; D058185:Magnetite Nanoparticles; D008422:Materials Testing; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000187:drug effects* / Q000502:physiology*; D053758:Nanoparticles / Q000737:chemistry*; D016877:Oxidants / Q000378:metabolism; D010084:Oxidation-Reduction; D010087:Oxides / Q000737:chemistry / Q000494:pharmacology*; D010544:Peroxidases / Q000378:metabolism
D003287:Contrast Media; D003911:Dextrans; D005290:Ferric Compounds; D058185:Magnetite Nanoparticles; D016877:Oxidants; D010087:Oxides; C000499:ferric oxide; D006861:Hydrogen Peroxide; D007501:Iron; D010544:Peroxidases; C065507:ferumoxides; D052203:Ferrosoferric Oxide