Validation of five-colony pool analysis using multiplex real-time PCR for detection of diarrheagenic Escherichia coli.
Five Escherichia coli colonies/patient were studied to evaluate the reliability of a multiplex real-time PCR assay for detection of diarrheagenic Escherichia coli groups, using a pool of five colonies rather than individual colonies. Sensitivity and specificity were 98% and 100%, respectively, at a fifth of the cost of the individual colony analysis.
Instituto de Medicina Tropical, Universidad Peruana Cayetano Heredia, Av. Honorio Delgado 340, San Martin de Porras, Lima 33, Perú. francescabarletta@yahoo.es
F
F
Barletta
T J
TJ
Ochoa
L
L
Ecker
A I
AI
Gil
C F
CF
Lanata
T G
TG
Cleary
D004926:Escherichia coli / Q000235:genetics / Q000302:isolation & purification*; D004927:Escherichia coli Infections / Q000175:diagnosis*; D006801:Humans; D007223:Infant; D016133:Polymerase Chain Reaction / Q000191:economics / Q000379:methods*; D012680:Sensitivity and Specificity