Use of non-radioactive detection method for north- and southwestern blot.
Many proteins bind to nucleic acids. For the first characterization of novel proteins, a fast and simple technique for testing their nucleic acid binding capabilities is desirable. Here, we describe the use of a northwestern and southwestern blot protocol for the evaluation of the DNA and RNA binding abilities of a novel putative methyl transferase HSPC133 (METTL5).
Franke
Claudia
C
Institute Immunology, MTZ, Carl Gustav Carus University TU Dresden, Medical Faculty, Fiedlerstrasse 42, D-01307, Dresden, Germany.
Gräfe
Daniel
D
Bartsch
Holger
H
Bachmann
Michael
M
D001324:Autoantigens / Q000737:chemistry / Q000378:metabolism; D026501:Blotting, Southwestern / Q000295:instrumentation / Q000379:methods*; D004247:DNA / Q000737:chemistry / Q000378:metabolism*; D015342:DNA Probes / Q000737:chemistry / Q000378:metabolism; D004076:Digoxigenin / Q000737:chemistry; D006801:Humans; D011485:Protein Binding; D011506:Proteins / Q000737:chemistry / Q000378:metabolism*; D012313:RNA / Q000737:chemistry / Q000378:metabolism*; D015347:RNA Probes / Q000737:chemistry / Q000378:metabolism; D012261:Ribonucleoproteins / Q000737:chemistry / Q000378:metabolism; D000097585:SS-B Antigen
D001324:Autoantigens; D004247:DNA; D015342:DNA Probes; D004076:Digoxigenin; D011506:Proteins; D012313:RNA; D015347:RNA Probes; D012261:Ribonucleoproteins; D000097585:SS-B Antigen