Selective protein expression within the CNS using hybrid lentivirus.
Lentiviral vectors offer many advantages within the central nervous system. They are capable of transfecting neurons and can be readily manipulated for selective expression. This chapter describes the production and use of lentiviruses to deliver GFP to the central nervous system.
Gussing
Fredrik
F
Department of Experimental Medical Science, Lund University, Lund, Sweden.
van Marion
Ingrid
I
Ralets
Igor
I
Lundberg
Cecilia
C
D002460:Cell Line; D002490:Central Nervous System / Q000378:metabolism*; D003001:Cloning, Molecular; D015870:Gene Expression; D005822:Genetic Vectors / Q000235:genetics*; D006801:Humans; D016086:Lentivirus / Q000235:genetics / Q000378:metabolism*; D011401:Promoter Regions, Genetic / Q000235:genetics; D011506:Proteins / Q000235:genetics / Q000378:metabolism*; D014162:Transfection / Q000379:methods*
D011506:Proteins