Ethylglucuronide determination in urine and hair from alcohol withdrawal patients.
Two methods for the determination of ethylglucuronide (EtG) in urine and in hair have been developed by liquid chromatography- tandem mass spectrometry. These two methods were fully validated, including linearity (0.25-100 microg/mL in urine; 0.05-5 ng/mg in hair; r(2) > 0.99, n = 5), limits of detection (0.1 microg/mL in urine, 0.025 ng/mg in hair) and quantitation (lowest level of the calibration curve), extraction efficiency (> 55%), within-day and between-day imprecision and bias (CV and mean relative error < 15%), matrix effect, and relative ion intensity. These methods have been applied to 541 urine samples and 17 hair specimens collected from 156 alcohol withdrawal patients. The determination of ethanol versus EtG in urine was compared, and also the convenience of EtG determination in hair. EtG in urine and in hair proved to be a powerful tool for monitoring abstinence in these patients.
Forensic Toxicology Service, Institute of Legal Medicine, University of Santiago de Compostela, C/ San Francisco s/n, 15782 Santiago de Compostela, Spain.
Marta
M
Concheiro
Angelines
A
Cruz
Marisol
M
Mon
Ana
A
de Castro
Oscar
O
Quintela
Angeles
A
Lorenzo
Manuel
M
López-Rivadulla
D000428:Alcohol Drinking / Q000652:urine*; D000437:Alcoholism / Q000175:diagnosis / Q000378:metabolism; D015415:Biomarkers / Q000032:analysis; D002853:Chromatography, Liquid; D000431:Ethanol / Q000032:analysis / Q000652:urine; D005554:Forensic Medicine; D005965:Glucuronates / Q000032:analysis / Q000652:urine*; D006197:Hair / Q000737:chemistry*; D006801:Humans; D015813:Substance Abuse Detection / Q000379:methods*; D013375:Substance Withdrawal Syndrome / Q000378:metabolism / Q000652:urine*; D053719:Tandem Mass Spectrometry
D015415:Biomarkers; D005965:Glucuronates; C093924:ethyl glucuronide; D000431:Ethanol