[Interleukin 2 promotes the expression of immunoglobulin G in a cervical cancer cell line HeLaS3].
OBJECTIVE
To analyze the effect of interleukin 2 (IL-2) on immunoglobulin G(IgG) expression in cervical cancer cell line HeLaS3.


METHODS
By immunofluorescence-flow cytometry(FCM), we detected membrane and cytoplasmic IgG expression variation of HeLaS3 after IL-2 stimulation. By Western blot, we detected IgG expression variation of HeLaS3 after IL-2 stimulation. Meanwhile, by RT-PCR with primers designed to amplify four subclasses of IgG, we detected Gamma transcript variation in HeLaS3 after IL-2 stimulation at mRNA level.


RESULTS
The result of immunofluorescence-FACS showed that after IL-2 stimulation for 48 hours, membrane and cytoplasmic IgG expression elevated in HeLaS3. The result of Western blot showed after IL-2 stimulation for 48 hours, IgG expression elevated in HeLaS3. Meanwhile, the result of RT-PCR showed that Gamma1 transcript mildly elevated after IL-2 stimulation for 48 hours.


CONCLUSIONS
IL-2 can promote IgG expression in cervical cancer cell line HeLaS3.
Department of Immunology, Peking University School of Basic Medical Sciences, Beijing 100191, China.
Tian
T
Lu
Xiao-hui
XH
Zhu
Shi-qing
SQ
Liu
Jie
J
Zheng
Xiao-yan
XY
Qiu
D005260:Female; D006367:HeLa Cells; D006801:Humans; D007074:Immunoglobulin G / Q000096:biosynthesis*; D007376:Interleukin-2 / Q000494:pharmacology*; D002583:Uterine Cervical Neoplasms / Q000276:immunology*
D007074:Immunoglobulin G; D007376:Interleukin-2