Two cases of failed ceftriaxone treatment in pharyngeal gonorrhoea verified by molecular microbiological methods.
Diagnostic, genotypic and antibiotic-resistance determinants of Neisseria gonorrhoeae were analysed by molecular methods to verify the failure of ceftriaxone treatment in two cases of pharyngeal gonorrhoea. Monoplex assays were needed to define competitive inhibition of a positive Chlamydia PCR in a duplex assay. Different penA changes were detected in the N. gonorrhoeae isolated from the two cases. These were associated with raised ceftriaxone MICs of 0.03 and 0.016 mg l(-1), which may have contributed to the treatment failures in these cases.
World Health Organization Collaborating Centre for STD, Prince of Wales Hospital, Sydney, Australia.
John
J
Tapsall
Sydney Sexual Health Centre, Sydney Hospital, Sydney, Australia.
Phillip
P
Read
Short Street Sexual Health Clinic, St George Hospital, Sydney, Australia.
Christopher
C
Carmody
Sydney Sexual Health Centre, Sydney Hospital, Sydney, Australia.
Christopher
C
Bourne
World Health Organization Collaborating Centre for STD, Prince of Wales Hospital, Sydney, Australia.
Sanghamitra
S
Ray
World Health Organization Collaborating Centre for STD, Prince of Wales Hospital, Sydney, Australia.
Athena
A
Limnios
Queensland Paediatric Infectious Diseases Laboratory, Sir Albert Sakzewski Virus Research Centre, Royal Children's Hospital and Health Service District, Queensland, Australia.
Theo
T
Sloots
Queensland Paediatric Infectious Diseases Laboratory, Sir Albert Sakzewski Virus Research Centre, Royal Children's Hospital and Health Service District, Queensland, Australia.
David
D
Whiley
D000328:Adult; D000900:Anti-Bacterial Agents / Q000627:therapeutic use; D002443:Ceftriaxone / Q000627:therapeutic use*; D002692:Chlamydia trachomatis / Q000235:genetics / Q000302:isolation & purification*; D005260:Female; D006069:Gonorrhea / Q000188:drug therapy*; D006716:Homosexuality; D006801:Humans; D008297:Male; D009344:Neisseria gonorrhoeae / Q000302:isolation & purification*; D010608:Pharyngeal Diseases / Q000188:drug therapy* / Q000382:microbiology*; D016133:Polymerase Chain Reaction; D017211:Treatment Failure
D000900:Anti-Bacterial Agents; D002443:Ceftriaxone