Ethanol metabolism in ALDH2 knockout mice--blood acetate levels.
We described here blood acetate levels in aldehyde dehydrogenase 2 knockout (ALDH2 KO) male mice based on C57BL/6J strain after ethanol (EtOH) dosing (2 g/kg). Blood samples were collected at 30, 60, 90, 120 180, and 240 min after decapitation, and then EtOH, acetaldehyde (AcH) and acetate were determined by head-space gas chromatography. We found that blood acetate levels in ALDH2 KO mice were slightly lower than those in wild type (WT), whereas EtOH and AcH levels in ALDH2 KO were significantly higher than those in WT. These observations indicate that high EtOH, AcH and low acetate in the blood of ALDH2 KO are due to the deficient effect of ALDH2 enzyme activity.
Department of Forensic Medicine, Faculty of Medicine, Kagawa University, 1750-1, Ikenobe, Miki, Kita, Kagawa 761-0793, Japan. amenok@kms.ac.jp
Ameno
A
Kiyoshi
Wang
W
Weihuan
Jamal
J
Mostofa
Kumihashi
K
Mitsuru
Isse
I
Toyoshi
Kawamoto
K
Toshihiro
Kitagawa
K
Kyoko
Nakayama
N
Keiichi
Ijiri
I
Iwao
Kinoshita
K
Hiroshi
D000079:Acetaldehyde / Q000097:blood; D000085:Acetates / Q000097:blood*; D000818:Animals; D002492:Central Nervous System Depressants / Q000097:blood / Q000493:pharmacokinetics*; D002849:Chromatography, Gas; D000431:Ethanol / Q000097:blood / Q000493:pharmacokinetics*; D053593:Forensic Toxicology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout
D000085:Acetates; D002492:Central Nervous System Depressants; D000431:Ethanol; D000079:Acetaldehyde