Transient expression of GFP in immature seed tissues.
Transient expression is the nonstable expression of a transgene whereby the transgene does not integrate into the host's genomic DNA. Transient expression assays have 20 years of history in plant molecular biology research, being used to answer a variety of questions. The method described here allows the ability to test promoter activity for seed-specific expression by quantifying reporter protein production in immature seed tissues. This method is especially suited to test vector activity for stable expression, test promoter activity, and discern regions of a promoter that is necessary for transcription in seed tissues. The transient expression assay is a tool that has aided a great deal of molecular biology research.
Shepherd
Colin T
CT
AimsBio, Inc., Ames, IA, USA. coshep@hotmail.com
D019470:Biolistics; D015870:Gene Expression; D005821:Genetic Techniques; D049452:Green Fluorescent Proteins / Q000096:biosynthesis* / Q000235:genetics*; D030821:Plants, Genetically Modified; D011401:Promoter Regions, Genetic; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics; D012639:Seeds / Q000235:genetics / Q000378:metabolism; D003313:Zea mays / Q000196:embryology / Q000235:genetics* / Q000378:metabolism*
D011994:Recombinant Proteins; D049452:Green Fluorescent Proteins