Effect of unoprostone on topographic and blood flow changes in the ischemic optic nerve head of rabbits.
OBJECTIVE
To determine whether subconjunctival injection of unoprostone isopropyl alters changes in the topography and blood flow of the optic disc induced by endothelin 1 (ET-1) in rabbits.


METHODS
From April 1, 2005, to April 28, 2006, we injected ET-1 (20 pmol) intravitreally into rabbits twice per week for 4 weeks. The observation period was 8 weeks. The first group received an intravitreal injection of ET-1 followed by a subconjunctival injection of unoprostone (0.12%, 50 microL). The second group received the same amount of ET-1 followed by a subconjunctival injection of the vehicle of unoprostone. The third group received the intravitreal vehicle of ET-1. The blood flow and topography of the optic nerve head (ONH) were measured by laser speckle flowgraphy and confocal scanning ophthalmoscopy, respectively. The number of cells in the retinal ganglion cell layer and inner nuclear layer was determined histologically.


RESULTS
We found that ET-1 decreased the ONH blood flow, decreased the cells in the ganglion cell layer and inner nuclear layer, enlarged the cup area of the ONH, and reduced the rim area of the ONH. When unoprostone was given with ET-1, no such changes occurred.


CONCLUSIONS
Unoprostone can suppress the effects of ET-1 on the circulation and topography of the ONH.


CONCLUSIONS
Unoprostone could be a candidate for treating eyes with ischemic ONH.
Department of Ophthalmology, Osaka Medical College, 2-7 Daigaku-machi, Takatsuki City 569-8686, Japan. tsugiyama@poh.osaka-med.ac.jp
Tetsuya
T
Sugiyama
Yukihiko
Y
Mashima
Yuriko
Y
Yoshioka
Hidehiro
H
Oku
Tsunehiko
T
Ikeda
D000818:Animals; D000959:Antihypertensive Agents / Q000008:administration & dosage*; D001783:Blood Flow Velocity; D002452:Cell Count; D002851:Chromatography, High Pressure Liquid; D015237:Dinoprost / Q000008:administration & dosage / Q000031:analogs & derivatives*; D019332:Endothelin-1 / Q000633:toxicity; D007267:Injections; D007429:Intraocular Pressure / Q000187:drug effects / Q000502:physiology; D017078:Laser-Doppler Flowmetry; D007834:Lasers; D008297:Male; D009887:Ophthalmoscopy; D009898:Optic Disk / Q000098:blood supply* / Q000187:drug effects; D018917:Optic Neuropathy, Ischemic / Q000503:physiopathology* / Q000517:prevention & control; D011817:Rabbits; D012039:Regional Blood Flow / Q000187:drug effects / Q000502:physiology; D012165:Retinal Ganglion Cells / Q000187:drug effects / Q000473:pathology; D012171:Retinal Vessels / Q000187:drug effects / Q000503:physiopathology*; D053719:Tandem Mass Spectrometry; D014065:Tonometry, Ocular; D014822:Vitreous Body
D000959:Antihypertensive Agents; D019332:Endothelin-1; C072630:isopropyl unoprostone; D015237:Dinoprost