Ovarian response to gonadotropin stimulation in juvenile rhesus monkeys.
The objective of this study was to investigate juvenile rhesus monkeys responding to various gonadotropin regimen stimulations. Thirty-two prepubertal rhesus monkeys were randomly allocated into five groups for ovarian stimulation as follows: Groups I, II, and III were given 35, 18, and 9 IU recombinant human follicle-stimulating hormone (rhFSH), respectively, twice daily for 8 d; Group IV was given 18 IU rhFSH twice daily until the appearance of maximal increase in sex skin during the breeding season; and Group V was treated identically to Group II but during the nonbreeding season. In addition, nine menarchial monkeys (Group VI) were treated identically to Group II. Menarchial monkeys yielded two- to fivefold the numbers of MII oocytes (24.1) and almost twice the development potential of in vitro-fertilized oocytes (blastocyst rate: 50.0%) compared with those of the other groups. Moreover, prepubertal monkeys in Group V had approximately double the numbers of MII oocytes and in Groups IV and V twice the development potential compared with those of Groups I and II, whereas Group III did not respond to stimulation. The most prominent sex skin swelling was in association with peak serum estradiol concentrations, and good responses to stimulation were associated with reduced body temperatures. All stimulated monkeys had normal reproductive performance at adulthood, except those in Group I. In conclusion, gonadotropin stimulation of menarchial monkeys could be appropriate for addressing the high cost and limited availability of rhesus monkeys in studying reproductive biology in primates.
Yang
S
S
Biotechnology Research Center, Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, People's Republic of China.
He
X
X
Niu
Y
Y
Hildebrandt
T B
TB
Jewgenow
K
K
Goeritz
F
F
Tang
X
X
Chang
Y
Y
Zhou
Q
Q
Ji
W
W
D000818:Animals; D001831:Body Temperature; D001947:Breeding; D006063:Chorionic Gonadotropin / Q000008:administration & dosage; D046149:Embryo Culture Techniques / Q000662:veterinary; D004958:Estradiol / Q000097:blood; D005260:Female; D005307:Fertilization in Vitro / Q000662:veterinary; D043373:Follicle Stimulating Hormone, Human / Q000008:administration & dosage*; D008253:Macaca mulatta / Q000502:physiology*; D009865:Oocytes / Q000254:growth & development; D010062:Ovulation Induction / Q000379:methods / Q000662:veterinary*; D010502:Perineum; D011247:Pregnancy; D011994:Recombinant Proteins / Q000008:administration & dosage; D012621:Seasons; D012741:Sexual Maturation; D012879:Skin Physiological Phenomena
D006063:Chorionic Gonadotropin; D043373:Follicle Stimulating Hormone, Human; D011994:Recombinant Proteins; D004958:Estradiol