Role of the parabrachial complex in the cardiorespiratory response evoked from hypothalamic defense area stimulation in the anesthetized rat.
To analyze the role of parabrachial complex (PBc) in the modulation of cardiorespiratory response evoked from the hypothalamic defense area (HDA), cardiorespiratory changes were analyzed in spontaneously breathing anesthetised rats in response to electrical stimulation of the HDA (1 ms pulses, 30-50 microA, 100 Hz for 5 s) before and after the microinjection of muscimol (50 nl, 0.25 nmol, 5 s) within the PBc. HDA stimulation evoked an inspiratory facilitatory response, consisting of an increase in respiratory rate (p<0.001) due to a decrease in expiratory time (p<0.01). The respiratory response was accompanied by a pressor (p<0.001) and a tachycardic (p<0.001) response. Muscimol microinjection within the lateral parabrachial region (lPB) abolished the respiratory response to HDA stimulation (p<0.01) and decreased the pressor response (p<0.05). Muscimol within the medial parabrachial region and Kölliker-Fuse (mPB-KF) decreased the magnitude of the pressor (p<0.01) and tachycardic (p<0.05) responses to HDA stimulation. The respiratory response persisted unchanged. Finally, extracellular recording of putative neurons from these regions were obtained during HDA stimulation to confirm functional interaction between HDA and parabrachial regions. 105 pontine cells were recorded during HDA stimulation, 57 from the lPB and 48 from the mPB-KF. In mPB-KF 34/48 (71%) and in lPB 38/57 (67%) cells were influenced from HDA. The results indicate that neurons from different regions of the PBc have an important function in mediating the cardiorespiratory response evoked from the HDA. The possible mechanisms involved in these interactions are discussed.
Departamento de Fisiología, Facultad de Medicina, Universidad de Málaga, Málaga, Spain.
Amelia
A
Díaz-Casares
Manuel Víctor
MV
López-González
Carlos Antonio
CA
Peinado-Aragonés
José Pablo
JP
Lara
Salvador
S
González-Barón
Marc Stefan
MS
Dawid-Milner
D000200:Action Potentials; D000758:Anesthesia; D000818:Animals; D001794:Blood Pressure / Q000502:physiology*; D001933:Brain Stem / Q000187:drug effects / Q000502:physiology*; D004558:Electric Stimulation; D018755:GABA Agonists / Q000494:pharmacology; D006339:Heart Rate / Q000502:physiology*; D007031:Hypothalamus / Q000502:physiology*; D008297:Male; D008839:Microelectrodes; D008845:Microinjections; D009118:Muscimol / Q000494:pharmacology; D009434:Neural Pathways / Q000187:drug effects / Q000502:physiology; D009474:Neurons / Q000502:physiology; D051381:Rats; D017207:Rats, Sprague-Dawley; D012119:Respiration
D018755:GABA Agonists; D009118:Muscimol