Hematologic, serum biochemical, and cortisol changes associated with anticipation of exercise and short duration high-intensity exercise in sled dogs.
BACKGROUND
Many studies have demonstrated changes in clinicopathologic variables in response to exercise in athletic dogs. These changes have been attributed to the duration and intensity of exercise. However, inconsistencies in the timing of collection of resting blood samples can be a confounding variable when interpreting results.


OBJECTIVE
The objectives of this study were to determine whether hematologic and biochemical data in sled dogs are influenced by (1) anticipation of an exercise event or (2) short-duration high-intensity exercise.


METHODS
Blood samples were collected into EDTA tubes and tubes without anticoagulant from sled dogs at rest, immediately before a 3-mile run (preexercise), and immediately after the run (postexercise). A CBC, biochemical profile (including electrolytes, glucose, proteins, creatinine, urea, enzymes, cholesterol, and total bilirubin), and serum cortisol concentration were measured using standard methods on automated analyzers.


RESULTS
Significant increases in sodium, chloride, albumin, calcium, and cortisol concentration were observed in preexercise samples when compared with resting samples. Hyperglycemia and a further rise in serum cortisol concentration occurred immediately postexercise.


CONCLUSIONS
The results of this study indicate that anticipation of exercise affects several biochemical analytes, emphasizing the importance of appropriate timing of baseline blood samples. The pronounced hyperglycemia immediately after short-duration exercise may be a breed- or exercise-dependent phenomenon.
Angle
Craig T
CT
College of Veterinary Medicine, Auburn University, Auburn, AL, USA.
Wakshlag
Joseph J
JJ
Gillette
Robert L
RL
Stokol
Tracy
T
Geske
Sue
S
Adkins
Terry O
TO
Gregor
Cara
C
D000418:Albumins; D000818:Animals; D001786:Blood Glucose / Q000502:physiology*; D004285:Dogs / Q000097:blood* / Q000502:physiology*; D004573:Electrolytes / Q000097:blood; D006854:Hydrocortisone / Q000097:blood*; D009994:Osmolar Concentration; D010805:Physical Conditioning, Animal / Q000502:physiology*; D011446:Prospective Studies
D000418:Albumins; D001786:Blood Glucose; D004573:Electrolytes; D006854:Hydrocortisone