Real-time monitoring of peptide cleavage using a nanopore probe.
Here we report a rapid, label-free method for monitoring peptide cleavage. Monitoring peptide translocation through an engineered ion channel in the absence and the presence of an enzyme allowed quantitative chemical kinetics information on enzymatic processes to be obtained. In addition to its potential application in disease diagnostics and drug discovery, this peptide/protein cleavage approach is envisioned for further development as a novel rapid, label-free protein sequencing technique.
Zhao
Qitao
Q
Department of Chemistry and Biochemistry, The University of Texas at Arlington, Arlington, Texas 76019-0065, USA.
de Zoysa
Ranulu Samanthi S
RS
Wang
Deqiang
D
Jayawardhana
Dilani A
DA
Guan
Xiyun
X
D004798:Enzymes / Q000378:metabolism*; D006868:Hydrolysis; D007473:Ion Channels / Q000138:chemical synthesis; D007700:Kinetics; D010455:Peptides / Q000378:metabolism*; D020539:Sequence Analysis, Protein / Q000379:methods
D004798:Enzymes; D007473:Ion Channels; D010455:Peptides