Effect of citral, eugenol, nerolidol and alpha-terpineol on the ultrastructural changes of Trichophyton mentagrophytes.
The antifungal effects of citral, eugenol, nerolidol and alpha-terpineol on Trichophyton mentagrophytes were investigated. Citral over 0.1 mg/ml strongly inhibited the hyphal growth of T. mentagrophytes, and the antifungal activity of alpha-terpineol was less effective. The morphological changes of the fungus exposed to the terpenes were observed by electron microscopy. The hyphae were distorted and collapsed at 0.2, 0.4 and 1 mg/ml of eugenol, nerolidol and alpha-terpineol respectively, and cell membrane and organelles were irreversibly damaged at 0.2 mg/ml citral. These suggested that four terpenes possess antifungal activity against T. mentagrophytes, and the activity might lead to irreversible cellular disruption.
Park
M J
MJ
Dept of Forest Sciences, Seoul National University, Gwanakgu, Seoul, Republic of Korea.
Gwak
K S
KS
Yang
I
I
Kim
K W
KW
Jeung
E B
EB
Chang
J W
JW
Choi
I G
IG
D000080462:Acyclic Monoterpenes; D000935:Antifungal Agents / Q000494:pharmacology*; D000081005:Cyclohexane Monoterpenes; D053138:Cyclohexenes / Q000494:pharmacology; D005054:Eugenol / Q000494:pharmacology*; D025301:Hyphae / Q000187:drug effects; D008854:Microscopy, Electron; D039821:Monoterpenes / Q000494:pharmacology; D010936:Plant Extracts / Q000494:pharmacology*; D010938:Plant Oils / Q000737:chemistry; D012717:Sesquiterpenes / Q000494:pharmacology; D013729:Terpenes / Q000494:pharmacology*; D014249:Trichophyton / Q000187:drug effects* / Q000254:growth & development / Q000648:ultrastructure
D000080462:Acyclic Monoterpenes; D000935:Antifungal Agents; D000081005:Cyclohexane Monoterpenes; D053138:Cyclohexenes; D039821:Monoterpenes; D010936:Plant Extracts; D010938:Plant Oils; D012717:Sesquiterpenes; D013729:Terpenes; C016775:alpha-terpineol; D005054:Eugenol; C037055:nerolidol; C007076:citral