Development and validation of an HPLC method to quantify 3,4-dideoxyglucosone-3-ene in peritoneal dialysis fluids.
A method was developed and validated to quantify 3,4-dideoxyglucosone-3-ene in peritoneal dialysis fluids by high-performance liquid chromatography with UV detection after derivatization with o-phenylenediamine. The advantages of this method compared with direct HPLC analysis are (i) the possibility of quantifying 3,4-dideoxyglucosone-3-ene simultaneously together with other glucose degradation products, (ii) the compatibility of the method with MS detection for unequivocal identification of the analyte and (iii) a bathochromic shift of the UV absorbance maximum which leads to higher selectivity. The validated method was used to measure 3,4-dideoxyglucosone-3-ene concentrations additionally to the glucose degradation products 3-deoxyglucosone, methylglyoxal, glyoxal, 5-hydroxymethylfurfural, 2-furaldehyde, formaldehyde and acetaldehyde in 19 commercial products for peritoneal dialysis.
Department of Chemistry and Pharmacy, Food Chemistry, University of Erlangen-Nuremberg, Erlangen, Germany.
Matthias
M
Frischmann
Johanna
J
Spitzer
Michael
M
Fünfrocken
Stefan
S
Mittelmaier
Melanie
M
Deckert
Thomas
T
Fichert
Monika
M
Pischetsrieder
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D015314:Dialysis Solutions / Q000032:analysis*; D005947:Glucose / Q000032:analysis* / Q000378:metabolism*; D006801:Humans; D016014:Linear Models; D010530:Peritoneal Dialysis; D010655:Phenylenediamines; D011753:Pyrones / Q000032:analysis*; D012680:Sensitivity and Specificity
C504711:3,4-dideoxyglucosone-3-ene; D015314:Dialysis Solutions; D010655:Phenylenediamines; D011753:Pyrones; C034193:1,2-diaminobenzene; D005947:Glucose