Three-dimensional localization of implanted biomaterials in anatomical and histological specimens using combined X-ray computed tomography and three-dimensional surface reconstruction: a technical note.
For adequate histological processing of implanted biomaterials or tissue-engineered constructs, it is sometimes essential to obtain insight into the localization of structures inside the tissue samples. Observation of three-dimensional (3D) surface reconstruction, including basic photorealistic texture characteristics as surface pattern and color combined with X-ray computed tomography 3D reconstruction at different levels, is a useful approach to localize anatomical or implanted structures within experimental tissue samples. Because of the possible observation of structures of interest in a 3D environment, fusion of these techniques can greatly facilitate histological processing.
Department of Periodontology and Biomaterials, Radboud University Nijmegen Medical Centre, Nijmegen, The Netherlands .
Vincent M J I
VM
Cuijpers
X Frank
XF
Walboomers
John A
JA
Jansen
D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D002138:Calibration; D006801:Humans; D007091:Image Processing, Computer-Assisted; D021621:Imaging, Three-Dimensional / Q000379:methods*; D010363:Pattern Recognition, Automated; D019736:Prostheses and Implants; D012984:Software; D013499:Surface Properties; D025321:Surgery, Computer-Assisted; D013552:Swine; D023822:Tissue Engineering / Q000379:methods; D014025:Titanium / Q000737:chemistry; D014057:Tomography, X-Ray Computed / Q000379:methods*
D001672:Biocompatible Materials; D014025:Titanium