DoG Picker and TiltPicker: software tools to facilitate particle selection in single particle electron microscopy.
Solving the structure of macromolecular complexes using transmission electron microscopy can be an arduous task. Many of the steps in this process rely strongly on the aid of pre-existing structural knowledge, and are greatly complicated when this information is unavailable. Here, we present two software tools meant to facilitate particle picking, an early stage in the single-particle processing of unknown macromolecules. The first tool, DoG Picker, is an efficient and reasonably general, particle picker based on the Difference of Gaussians (DoG) image transform. It can function alone, as a reference-free particle picker with the unique ability to sort particles based on size, or it can also be used as a way to bootstrap the creation of templates or training datasets for other particle pickers. The second tool is TiltPicker, an interactive graphical interface application designed to streamline the selection of particle pairs from tilted-pair datasets. In many respects, TiltPicker is a re-implementation of the SPIDER WEB tilted-particle picker, but built on modern computer frameworks making it easier to deploy and maintain. The TiltPicker program also includes several useful new features beyond those of its predecessor.
Voss
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National Resource for Automated Molecular Microscopy and Department of Cell Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, CB-129, La Jolla, CA 92037, USA.
Yoshioka
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Radermacher
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Potter
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Carragher
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D046529:Microscopy, Electron, Transmission / Q000379:methods*; D012984:Software