Fluorescence and electron microscopic localization of F-actin in the ependymocytes.
The organization of F-actin in the ventricular system has been reported to display pronounced regional differences with respect to shape, size, and development. However, the real roles played by F-actin in these cells cannot be understood unless the precise localization of F-actin is defined. In the present study, we used double-fluorescence labeling to further examine the localization of F-actin in the ependymocytes and its spatial relation to the other two cytoskeletal components, microtubules and intermediate filaments. Then we converted fluorescence signals for F-actin to peroxidase/DAB reaction products by use of a phalloidin-based FITC-anti-FITC system. This detection technique provided an overview of the distribution of F-actin in the ependymocytes at the ultrastructural level, and has been proven to be helpful in correlating light and electron microscopic investigations.
Neural Architecture, Advanced Technology Development Group, RIKEN Brain Science Institute, Hirosawa 2-1, Wako, Saitama 351-0198, Japan. ldlyc@brain.riken.jp
Yan-Chao
YC
Li
Wan-Zhu
WZ
Bai
Kazuhisa
K
Sakai
Tsutomu
T
Hashikawa
D015100:3,3'-Diaminobenzidine; D000199:Actins / Q000378:metabolism*; D000818:Animals; D002831:Choroid Plexus / Q000166:cytology / Q000378:metabolism / Q000648:ultrastructure; D004805:Ependyma / Q000166:cytology / Q000378:metabolism* / Q000648:ultrastructure; D005260:Female; D016650:Fluorescein-5-isothiocyanate; D005456:Fluorescent Dyes; D007382:Intermediate Filaments / Q000378:metabolism; D008854:Microscopy, Electron; D008856:Microscopy, Fluorescence; D008870:Microtubules / Q000378:metabolism; D010544:Peroxidases; D051381:Rats; D017207:Rats, Sprague-Dawley; D013116:Spinal Cord / Q000166:cytology / Q000378:metabolism / Q000648:ultrastructure
D000199:Actins; D005456:Fluorescent Dyes; D015100:3,3'-Diaminobenzidine; D010544:Peroxidases; D016650:Fluorescein-5-isothiocyanate