Conserved and novel miRNAs in the legume Phaseolus vulgaris in response to stress.
MicroRNAs (miRNAs) are small RNA molecules recognized as important regulators of gene expression. Although plant miRNAs have been extensively studied in model systems, less is known in other plants with limited genome sequence data. We are interested in the identification of miRNAs in Phaseolus vulgaris (common bean) to uncover different plant strategies to cope with adverse conditions and because of its relevance as a crop in developing countries. Here we present the identification of conserved and candidate novel miRNAs in P. vulgaris present in different organs and growth conditions, including drought, abscisic acid treatment, and Rhizobium infection. We also identified cDNA sequences in public databases that represent the corresponding miRNA precursors. In addition, we predicted and validated target mRNAs amongst reported EST and cDNAs for P. vulgaris. We propose that the novel miRNAs present in common bean and other legumes, are involved in regulation of legume-specific processes including adaptation to diverse external cues.
Arenas-Huertero
Catalina
C
Departamento de Biología Molecular de Plantas, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Av. Universidad 2001, Cuernavaca, Morelos, Mexico.
Pérez
Beatriz
B
Rabanal
Fernando
F
Blanco-Melo
Daniel
D
De la Rosa
Carlos
C
Estrada-Navarrete
Georgina
G
Sanchez
Federico
F
Covarrubias
Alejandra Alicia
AA
Reyes
José Luis
JL
D000040:Abscisic Acid / Q000494:pharmacology; D001483:Base Sequence; D015152:Blotting, Northern; D003080:Cold Temperature; D017124:Conserved Sequence; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D015723:Gene Library; D035683:MicroRNAs / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D027805:Phaseolus / Q000187:drug effects / Q000235:genetics*; D010937:Plant Growth Regulators / Q000494:pharmacology; D018515:Plant Leaves / Q000187:drug effects / Q000235:genetics; D018517:Plant Roots / Q000187:drug effects / Q000235:genetics; D018749:RNA, Plant / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D012639:Seeds / Q000187:drug effects / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid; D012965:Sodium Chloride / Q000494:pharmacology; D013025:Glycine max / Q000235:genetics; D014867:Water / Q000494:pharmacology
D035683:MicroRNAs; D010937:Plant Growth Regulators; D018749:RNA, Plant; D014867:Water; D012965:Sodium Chloride; D000040:Abscisic Acid