VirB alleviates H-NS repression of the icsP promoter in Shigella flexneri from sites more than one kilobase upstream of the transcription start site.
The icsP promoter of Shigella spp. is repressed by H-NS and derepressed by VirB. Here, we show that an inverted repeat located between positions -1144 and -1130 relative to the icsP transcription start site is necessary for VirB-dependent derepression. The atypical location of this cis-acting site is discussed.
School of Life Sciences, University of Nevada, Las Vegas, NV 89154-4004, USA.
Maria I
MI
Castellanos
Dustin J
DJ
Harrison
Jennifer M
JM
Smith
Stephanie K
SK
Labahn
Karen M
KM
Levy
Helen J
HJ
Wing
D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D015536:Down-Regulation; D015964:Gene Expression Regulation, Bacterial; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D012763:Shigella flexneri / Q000201:enzymology / Q000235:genetics* / Q000378:metabolism; D024363:Transcription Initiation Site; D037521:Virulence Factors / Q000235:genetics / Q000378:metabolism*
D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; C031908:H-NS protein, bacteria; D037521:Virulence Factors; C060971:SopA protein, Bacteria