Maté attenuates DNA damage and carcinogenesis induced by diethylnitrosamine and thermal injury in rat esophagus.
Drinking hot maté has been associated with risk for esophageal cancer in South America. Thus, the aims of this study were to evaluate the modifying effects of maté intake on DNA damage and esophageal carcinogenesis induced by diethylnitrosamine (DEN) and thermal injury (TI) in male Wistar rats. At the initiation phase of carcinogenesis, rats were treated with DEN (8 x 80 mg/kg) and submitted to TI (water at 65 degrees C, 1 ml/rat, instilled into the esophagus). Concomitantly, the animals received maté (2.0%w/v) for 8 weeks. Samples of peripheral blood were collected 4h after the last DEN application for DNA damage analysis. At weeks 8 and 20, samples from esophagus and liver were also collected for histological and immunohistochemical analysis. Maté significantly decreased DNA damage in leukocytes, cell proliferation rates in both esophagus and liver and the number of preneoplastic liver lesions from DEN/TI-treated animals at week 8. A significant lower incidence of esophageal papillomas and liver adenomas and tumor multiplicity was observed in the animals previously treated with maté at week 20. Thus, maté presented protective effects against DNA damage and esophageal and liver carcinogenesis induced by DEN.
UNESP São Paulo State University, Institute of Biosciences, Department of Morphology, 18618-000 Botucatu, SP, Brazil.
Juliana Ferreira da
JF
Silva
Lucas Tadeu
LT
Bidinotto
Kelly Silva
KS
Furtado
Daisy Maria Fávero
DM
Salvadori
Diogo Pineda
DP
Rivelli
Silvia Berlanga de Moraes
SB
Barros
Maria Aparecida Marchesan
MA
Rodrigues
Luis Fernando
LF
Barbisan
D000818:Animals; D016588:Anticarcinogenic Agents; D029063:Aquifoliaceae / Q000737:chemistry*; D001219:Aspartate Aminotransferases / Q000097:blood; D001628:Beverages; D001835:Body Weight / Q000187:drug effects; D002056:Burns / Q000150:complications* / Q000473:pathology; D002110:Caffeine / Q000494:pharmacology; D020552:Comet Assay; D004249:DNA Damage; D004052:Diethylnitrosamine / Q000037:antagonists & inhibitors* / Q000633:toxicity; D004938:Esophageal Neoplasms / Q000473:pathology / Q000517:prevention & control*; D004947:Esophagus / Q000293:injuries*; D005979:Glutathione Peroxidase / Q000378:metabolism; D007150:Immunohistochemistry; D007962:Leukocytes / Q000187:drug effects / Q000473:pathology / Q000648:ultrastructure; D008297:Male; D009929:Organ Size / Q000187:drug effects; D018809:Proliferating Cell Nuclear Antigen / Q000032:analysis; D020011:Protective Agents; D051381:Rats; D017208:Rats, Wistar; D013662:Tea
D016588:Anticarcinogenic Agents; D018809:Proliferating Cell Nuclear Antigen; D020011:Protective Agents; D013662:Tea; D002110:Caffeine; D004052:Diethylnitrosamine; D005979:Glutathione Peroxidase; D001219:Aspartate Aminotransferases