Correlation of inhibitor of differentiation 1 expression to tumor progression, poor differentiation and aggressive behaviors in cervical carcinoma.
OBJECTIVE
To study the correlation of inhibitor of differentiation 1 (Id-1) to tumor invasion and metastasis by examining Id-1 expression levels in different stages of cervical carcinogenesis.


METHODS
Id-1 mRNA and protein expression was detected in total of 171 cervical samples including precancerous and cancerous tissues by quantitative RT-PCR (qRT-PCR) and immunohistochemistry (IHC), respectively. Twenty-five normal cervical tissues were used as a normal control. Correlation between Id-1 positive rates and expression levels to cancer progression and clinicopathologic features was statistically analyzed.


RESULTS
A gradual increase of Id-1 protein expression associated with cervical cancer progression was detected (4%, 16%, 50% and 75.9% in normal, low squamous intraepithelial lesion (LSIL), high squamous intraepithelial lesion (HSIL) and cancer tissue, respectively, p<0.001). A similar trend of Id-1 mRNA expression was also observed (1.3, 3.4 and 10.4 fold higher than normal tissues in LSIL, HSIL and cancer tissue, respectively, p<0.001). Furthermore, the Id-1 expression level was correlated to tumor grade (p=0.005), lymph node metastasis (p=0.001), interstitial invasive (p<0.001) and tumor size (p<0.001). These results suggest that high Id-1 expression is associated with tumor growth, invasion and metastasis.


CONCLUSIONS
Id-1 expression is correlated to progression and aggressive behaviors in cervical cancer, suggesting a tumor-promoting role for Id-1 in progression of this malignancy.
Li
Jinke
J
Department of Gynecology and Obstetrics, West China Second Hospital, Sichuan University, 20 Renmingnan Road, Chengdu, Sichuan 610041, China.
Jia
Haijun
H
Xie
Lingxia
L
Wang
Xia
X
He
Haining
H
Lin
Yong
Y
Hu
Lina
L
D000374:Aggression; D002294:Carcinoma, Squamous Cell / Q000235:genetics / Q000473:pathology / Q000523:psychology / Q000601:surgery; D002454:Cell Differentiation; D017931:DNA Primers; D018450:Disease Progression; D005260:Female; D055452:Growth Differentiation Factor 1 / Q000235:genetics*; D006801:Humans; D007044:Hysterectomy; D007150:Immunohistochemistry; D008197:Lymph Node Excision; D009361:Neoplasm Invasiveness; D009362:Neoplasm Metastasis; D012333:RNA, Messenger / Q000235:genetics; D012334:RNA, Neoplasm / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D002583:Uterine Cervical Neoplasms / Q000235:genetics / Q000473:pathology* / Q000523:psychology* / Q000601:surgery
D017931:DNA Primers; D055452:Growth Differentiation Factor 1; D012333:RNA, Messenger; D012334:RNA, Neoplasm