[Preliminary study of p53 gene promoter binding site of activator protein-1 in A549 cell lines by chromatin immunoprecipitation].
OBJECTIVE
To detect and verify activator protein-1 (AP-1) binding to p53 gene promoter region in A549 cell lines in vitro.


METHODS
AP-1 binding to p53 gene promoter region was detected by chromatin immunoprecipitation (ChIP) assay using c-jun specific antibody, and PCR amplified its gene specific DNA fragment.


RESULTS
The p53 gene specific fragment was found in the DNA fragment immunoprecipitated by c-jun antibody.


CONCLUSIONS
AP-1 binds to p53 gene promoter region in A549 cells, and regulates its expression.
Department of Occupational Health and Environmental Health, Public Health College, Zhengzhou University, Zhengzhou, Henan Province 450001, China.
Fei
F
Shen
Zhi-tao
ZT
Li
Yi-ming
YM
Wu
Wei-dong
WD
Wu
D045744:Cell Line, Tumor; D047369:Chromatin Immunoprecipitation; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic / Q000235:genetics*; D011485:Protein Binding; D018808:Transcription Factor AP-1 / Q000235:genetics*; D016159:Tumor Suppressor Protein p53 / Q000235:genetics*
C495901:TP53 protein, human; D018808:Transcription Factor AP-1; D016159:Tumor Suppressor Protein p53