Loss of vancomycin bactericidal activity against accessory gene regulator (agr) dysfunctional Staphylococcus aureus under conditions of high bacterial density.
The impact of accessory gene regulator (agr) dysfunction and high bacterial density on vancomycin killing and resistance was evaluated among 10 clinical methicillin-resistant Staphylococcus aureus bloodstream isolates using time kill experiments. Under conditions of high inocula and agr dysfunction, vancomycin activity was markedly attenuated, amplifying resistant mutants by 72 h.
The New York State Center of Excellence in Bioinformatics & Life Sciences, University at Buffalo, New York 14205, USA. btsuji@buffalo.edu
Brian T
BT
Tsuji
Yoriko
Y
Harigaya
Alan J
AJ
Lesse
George
G
Sakoulas
Joseph M
JM
Mylotte
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D016470:Bacteremia / Q000382:microbiology; D001426:Bacterial Proteins / Q000378:metabolism*; D015169:Colony Count, Microbial; D006801:Humans; D055624:Methicillin-Resistant Staphylococcus aureus / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification; D050296:Microbial Viability / Q000187:drug effects*; D009154:Mutation; D013203:Staphylococcal Infections / Q000382:microbiology; D013997:Time Factors; D015534:Trans-Activators / Q000378:metabolism*; D014640:Vancomycin / Q000494:pharmacology*; D020713:Vancomycin Resistance
C095698:Agr protein, Staphylococcus aureus; D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D015534:Trans-Activators; D014640:Vancomycin