Cervical heterotopic kidney transplantation in rats using non-suturing and preserving-bag techniques.
BACKGROUND
This study describes a simple and stable cervical heterotopic kidney transplantation method in rats that uses artery sleeve anastomosis, vein cuff anastomosis and preserving-bag techniques.


METHODS
The donor graft, consisting of kidney, renal vein (RV), renal artery (RA) and a ureterocystic flap, was removed en bloc and perfused in situ. The donor RA was end-to-end anastomosed to the recipient left common carotid artery (CCA) using a sleeve anastomosis, and the donor RV was connected to the recipient right external jugular vein (EJV) using a cuff technique. During the vascular anastomosis, the kidney graft was placed in a lactated Ringer's solution ice-water preserving bag. The donor bladder patch was exteriorized to form cervical cutaneous stoma.


RESULTS
A total of 104 heterotopic renal transplantations were performed, which included pre-experimental (62 operations) and experimental stages (42 operations). The success rates of the two stages were 80.6% and 95.2%, respectively. The time for surgery was 40 +/- 6 min, the average time for donor surgery was 20 +/- 5 min, the preparation time for the graft was 8 +/- 2 min, the operative time for the recipient was 18 +/- 3 min that included the time for the arterial anastomosis (5 +/- 2 min) and venous anastomosis (2 +/- 1 min), the cold ischaemic time of the graft was 15 +/- 3 min and the warm ischaemic time of the graft was 2 +/- 1 min.


CONCLUSIONS
We developed an easy and reliable model of cervical heterotopic kidney transplantation that may provide a useful tool for investigating kidney transplantation rejection and retransplantation pathology.
Department of Physiology, Dalian Medical University, Liaoning Province, China.
Liang
L
Zhu
DeZheng
D
Gong
Yuan
Y
Zou
YouSheng
Y
Li
YunHong
Y
Wu
LiLi
L
Guan
Qiong
Q
Wu
Bo
B
Yuan
LiMing
L
Wang
FuWen
F
Luo
D000714:Anastomosis, Surgical / Q000379:methods; D000818:Animals; D016030:Kidney Transplantation / Q000379:methods* / Q000473:pathology; D008297:Male; D009333:Neck; D009926:Organ Preservation / Q000295:instrumentation / Q000379:methods; D019188:Organ Preservation Solutions; D051381:Rats; D017208:Rats, Wistar; D013536:Suture Techniques; D013997:Time Factors; D016042:Transplantation, Heterotopic; D014185:Transplantation, Isogeneic
D019188:Organ Preservation Solutions