Characterizing transgene inheritance.
The analysis of transgene inheritance is an important step in the molecular and genetic characterization of transgenes. In this manuscript, two approaches to characterize the inheritance of transgenes are described. The first approach is based on the expression of the transgene phenotype and the second is based on the analysis of transgene DNA. Instructions on how to make crosses and develop breeding populations are outlined and the importance of these breeding populations in the analysis of transgene inheritance is explained. The number of individuals needed to determine segregation ratios and the statistic used to test these ratios are described. Examples of inheritance patterns that deviate from known expectations are provided and the possible causes of these deviations are discussed.
Bicar
Earl H
EH
Monsanto, Kihei, HI 96753, USA. earl.h.bicar@monsanto.com
D000818:Animals; D015153:Blotting, Western; D001947:Breeding; D003433:Crosses, Genetic; D004274:DNA, Recombinant / Q000235:genetics; D015870:Gene Expression; D005821:Genetic Techniques; D007768:Lactalbumin / Q000096:biosynthesis / Q000235:genetics / Q000302:isolation & purification; D010641:Phenotype; D030821:Plants, Genetically Modified; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics / Q000302:isolation & purification; D013552:Swine; D003313:Zea mays / Q000235:genetics* / Q000378:metabolism
D004274:DNA, Recombinant; D011994:Recombinant Proteins; D007768:Lactalbumin