Peptide microarrays for determination of cross-reactivity.
Polyclonal antibodies raised against full-length antigens are often used for localization experiments. Exact knowledge of epitopes in the antigen recognized by the antiserum is important if the target antigen belongs to a large family of proteins which are highly conserved. We have shown that epitope mapping using peptide microarrays represents a powerful tool for determination of immunodominat regions in a proteome-wide manner. As examples we show results of epitope mapping using peptide microarrays displaying overlapping peptide scans through either all human cyclophilins or all human FK506-binding proteins.
Thiele
Alexandra
A
Max Planck Research Unit for Enzymology of Protein Folding, Weinbergweg 22, 06120 Halle, Germany.
D000595:Amino Acid Sequence; D000906:Antibodies / Q000737:chemistry / Q000276:immunology*; D003429:Cross Reactions; D021983:Cyclophilins / Q000737:chemistry / Q000276:immunology; D018604:Epitope Mapping / Q000379:methods*; D006801:Humans; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry / Q000276:immunology*; D019696:Peptidylprolyl Isomerase / Q000737:chemistry / Q000276:immunology; D040081:Protein Array Analysis / Q000379:methods*; D017434:Protein Structure, Tertiary; D016415:Sequence Alignment; D022021:Tacrolimus Binding Proteins / Q000737:chemistry / Q000276:immunology
D000906:Antibodies; D010455:Peptides; D021983:Cyclophilins; D022021:Tacrolimus Binding Proteins; D019696:Peptidylprolyl Isomerase