Towards tailor-made oligosaccharides-chemo-enzymatic approaches by enzyme and substrate engineering.
Carbohydrate structures have been identified in eukaryotic and prokaryotic cells as glycoconjugates with communication skills. Their recently discussed role in various diseases has attracted high attention in the development of simple and convenient methods for oligosaccharide synthesis. In this review, recent approaches combining nature's power for the design of tailor made biocatalysts by enzyme engineering and substrate engineering will be presented. These strategies lead to highly efficient and selective glycosylation reactions. The introduced concept shall be a first step in the direction to a glycosylation toolbox which paves the way for the tailor-made synthesis of designed carbohydrate structures.
Homann
Arne
A
Institute of Organic Chemistry, University of Würzburg, Am Hubland, D-97074, Würzburg, Germany.
Seibel
Jürgen
J
D001426:Bacterial Proteins / Q000737:chemistry / Q000378:metabolism*; D002236:Carbohydrate Conformation; D006031:Glycosylation; D016695:Glycosyltransferases / Q000737:chemistry / Q000378:metabolism*; D009844:Oligosaccharides / Q000138:chemical synthesis / Q000737:chemistry*; D015202:Protein Engineering; D013379:Substrate Specificity
D001426:Bacterial Proteins; D009844:Oligosaccharides; D016695:Glycosyltransferases