Antimicrobial action of a filling paste used in pulp therapy in primary teeth under different storage conditions.
OBJECTIVE
Compare the antimicrobial effectiveness of common antimicrobial paste immediately after pulp therapy and in different storage conditions.


METHODS
Staphylococcus aureus, Staphylococcus epidermis, Streptococcus mutans, Streptococcus oralis, Enterococcus faecalis; Escherichia coli and Bacillus subtilis sample were used. The storage conditions utilized were: at room temperature--the paste was subjected to a natural aging process at room temperature for a 24-hour and 7, 14, 28, 60 and 90 day periods (GPRT). Refrigerator storage--the paste was placed to a natural aging process in a refrigerator at 4 degrees C for a 24 hour and 7.14, 28, 60 and 90 day periods (GPR). To perform the immediate effect analysis (GPi or zero time), of each mixture either at room or refrigerator temperature, the preparation procedure was similar, although GPR, iodoform, Rifocort and camphorated paramonochlorophenol were stored in the refrigerator and then taken out, dosed and manipulated, and had their immediate effect tested after a 1-week period at storage of 4 degrees C average temperature. The testing methodology was Dilution in Solid Medium-Agar. The Wilcoxon Test and Friedman variance analyses were used.


RESULTS
The GP paste showed antimicrobial activity at all experimental times.


CONCLUSIONS
The pastes presented antimicrobial effectiveness at all experimental times.
Praetzel
Juliana Rodrigues
JR
School of Dentistry, Department of Stomatology, Federal University of Santa Maria, Rio Grande do Sul, Brazil.
Ferreira
Fabiana Vargas
FV
Weiss
Rita Niederauer
RN
Friedrich
Rosane Salete
RS
Guedes-Pinto
Antonio Carlos
AC
D000891:Anti-Infective Agents, Local / Q000494:pharmacology*; D001412:Bacillus subtilis / Q000187:drug effects; D002164:Camphor / Q000494:pharmacology; D002648:Child; D002733:Chlorophenols / Q000494:pharmacology; D015169:Colony Count, Microbial; D019140:Dental Care for Children / Q000379:methods; D003786:Dental Pulp Cavity / Q000187:drug effects / Q000382:microbiology*; D004338:Drug Combinations; D013293:Enterococcus faecalis / Q000187:drug effects; D004926:Escherichia coli / Q000187:drug effects; D006801:Humans; D006847:Hydrocarbons, Iodinated / Q000494:pharmacology; D011092:Polyethylene Glycols / Q000494:pharmacology; D011239:Prednisolone / Q000031:analogs & derivatives / Q000494:pharmacology / Q000627:therapeutic use; D011670:Pulpectomy / Q000379:methods; D012294:Rifamycins / Q000494:pharmacology / Q000627:therapeutic use; D012387:Root Canal Filling Materials / Q000494:pharmacology*; D012389:Root Canal Obturation / Q000379:methods*; D013211:Staphylococcus aureus / Q000187:drug effects; D013212:Staphylococcus epidermidis / Q000187:drug effects; D018709:Statistics, Nonparametric; D013295:Streptococcus mutans / Q000187:drug effects; D019533:Streptococcus oralis / Q000187:drug effects; D013997:Time Factors; D014094:Tooth, Deciduous
D000891:Anti-Infective Agents, Local; D002733:Chlorophenols; D004338:Drug Combinations; D006847:Hydrocarbons, Iodinated; D012294:Rifamycins; D012387:Root Canal Filling Materials; C541391:rifocort; D011092:Polyethylene Glycols; D002164:Camphor; C029804:camphorated parachlorophenol; D011239:Prednisolone; C010473:iodoform