Cadherin-13 in primary and blast crisis chronic myeloid leukaemia: declining expression and negative correlation with the BCR/ABL fusion gene.
Expression of the CDH13 gene in chronic myeloid leukaemia (CML) patients at different clinical stages, and its relationship with the BCR/ABL fusion gene, is investigated. Expression of the CDH13 gene and the BCR/ABL fuse gene is investigated in peripheral blood from 30 healthy adults, 25 primary CML patients and 25 CML patients in blast crisis using the EvaGreen real-time reverse transcriptase polymerase chain reaction (RT-PCR). Results showed that BCR/ABL fusion gene expression in the blast crisis CML patients was 4.72-fold higher than that in patients with primary CML. Expression of CDH13 mRNA in primary and blast crisis CML patients was lower than in the healthy adults, reduced 64% and 75%, respectively. Expression of CDH13 showed a negative correlation with the BCR/ABL fusion gene. The data indicate that the decline of CDH13 expression accompanied the different clinical stages of CML and probably was involved in over-expression of the BCR/ABL fusion gene.
Central Laboratory, Wuxi People's Hospital, Affiliated Nanjing Medical University, PR China. wxmuhj@yahoo.com.cn
H J
HJ
Mu
R
R
Xie
Y F
YF
Shen
Y Q
YQ
Jiang
Y J
YJ
Zeng
D000328:Adult; D001752:Blast Crisis / Q000097:blood / Q000235:genetics* / Q000473:pathology; D015820:Cadherins / Q000097:blood / Q000235:genetics*; D018450:Disease Progression; D004587:Electrophoresis, Agar Gel; D016044:Fusion Proteins, bcr-abl / Q000097:blood / Q000235:genetics*; D015973:Gene Expression Regulation, Leukemic; D016147:Genes, Tumor Suppressor; D006801:Humans; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000097:blood / Q000235:genetics* / Q000473:pathology; D008297:Male; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods
D015820:Cadherins; C099972:H-cadherin; C406060:abl-bcr fusion protein, human; D016044:Fusion Proteins, bcr-abl