epg-1 functions in autophagy-regulated processes and may encode a highly divergent Atg13 homolog in C. elegans.
Autophagy is an evolutionarily conserved intracellular catabolic system for degradation of long-lived proteins or damaged organelles. In this study, we have identified and characterized a new gene, epg-1, that plays a role in the autophagy pathway in C. elegans. Loss of function of epg-1 causes defects in various autophagy-regulated processes, including degradation of aggregate-prone proteins and optimal survival of animals during starvation. epg-1 encodes a novel protein that shows limited sequence similarity to the yeast autophagy protein Atg13. epg-1 displays a similar expression pattern to, and directly interacts with, the C. elegans Atg1 homolog UNC-51, suggesting that epg-1 encodes a divergent functional homolog of Atg13 in C. elegans.
Tian
E
E
College of Life Sciences, China Agricultural University, Beijing, China.
Wang
Fuxin
F
Han
Jinghua
J
Zhang
Hong
H
D048868:Adaptor Proteins, Signal Transducing / Q000737:chemistry* / Q000378:metabolism*; D000595:Amino Acid Sequence; D000818:Animals; D001343:Autophagy / Q000502:physiology*; D000071183:Autophagy-Related Proteins; D017173:Caenorhabditis elegans / Q000166:cytology* / Q000378:metabolism*; D029742:Caenorhabditis elegans Proteins / Q000737:chemistry / Q000378:metabolism*; D002352:Carrier Proteins / Q000737:chemistry / Q000378:metabolism; D003594:Cytoplasmic Granules / Q000378:metabolism; D019143:Evolution, Molecular; D049452:Green Fluorescent Proteins / Q000378:metabolism; D002479:Inclusion Bodies / Q000378:metabolism; D008969:Molecular Sequence Data; D009154:Mutation / Q000235:genetics; D009410:Nerve Degeneration / Q000473:pathology; D010455:Peptides / Q000378:metabolism; D011485:Protein Binding; D011499:Protein Processing, Post-Translational; D020836:Protein Structure, Quaternary; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry*; D017386:Sequence Homology, Amino Acid; D016019:Survival Analysis; D014110:Touch
C413216:ATG13 protein, S cerevisiae; D048868:Adaptor Proteins, Signal Transducing; D000071183:Autophagy-Related Proteins; D029742:Caenorhabditis elegans Proteins; D002352:Carrier Proteins; D010455:Peptides; D011993:Recombinant Fusion Proteins; C534958:SEPA-1 protein, C elegans; D029701:Saccharomyces cerevisiae Proteins; C543517:atg-13 protein, C elegans; D049452:Green Fluorescent Proteins; C097188:polyglutamine