Comparison of culture techniques at different stages of brucellosis.
The lysis centrifugation technique is preferred for culturing Brucella spp. at all stages of brucellosis because it yields 25% more positive results and on average provides results 10 days earlier than the Ruiz-Castaneda method. This lysis method is inexpensive and easier to use and may be used in laboratories with limited expertise or equipment if all safety precautions are taken.
Espinosa
Benjamin J
BJ
Naval Medical Research Center Detachment, Lima, Peru. benjamin.espinosa@med.navy.mil
Chacaltana
Jesús
J
Mulder
Maximilian
M
Franco
María Pía
MP
Blazes
David L
DL
Gilman
Robert H
RH
Smits
Henk L
HL
Hall
Eric R
ER
D001431:Bacteriological Techniques / Q000379:methods*; D002002:Brucella / Q000302:isolation & purification*; D002006:Brucellosis / Q000175:diagnosis / Q000382:microbiology*; D006801:Humans; D011237:Predictive Value of Tests; D012680:Sensitivity and Specificity; D012698:Serologic Tests