Controlled photopolymerization of hydrogel microstructures inside microchannels for bioassays.
This paper describes a photolithography approach to generate hydrogel microstructures inside microchannels on the basis of controlled photopolymerization of poly(ethylene glycol) diacrylate precursor by using a fluorescence microscope. The controlled photopolymerization offers: (1) morphology control on hydrogel microstructures with different illumination conditions; (2) position control on hydrogel microstructures to make it possible for the generation of arrayed hydrogel microstructures and encapsulation of single cells; (3) a convenient approach for immobilization of a variety of materials onto the solid substrates for bioassays. For demonstration, we performed a microfluidic assay for DNA oligomer detection on the arrayed hydrogel microstructures.
Liu
Jiangjiang
J
The Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology, Department of Chemistry, Tsinghua University, Beijing, 100084, China. jmlin@mail.tsinghua.edu.cn
Gao
Dan
D
Li
Hai-Fang
HF
Lin
Jin-Ming
JM
D001681:Biological Assay / Q000295:instrumentation*; D015374:Biosensing Techniques / Q000295:instrumentation*; D018929:Cell Culture Techniques / Q000295:instrumentation*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D020100:Hydrogels / Q000737:chemistry* / Q000528:radiation effects; D008027:Light; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D008904:Miniaturization; D010777:Photochemistry / Q000379:methods*
D020100:Hydrogels