Influence of dietary vitamin E supplementation on meat quality traits and gene expression related to lipid metabolism in the Beijing-you chicken.
1. The effects of dietary vitamin E (DL-alpha-tocopheryl acetate) on carcase and meat quality, oxidative stability, fatty acid composition of muscle lipids, and gene expression related to lipid metabolism were studied in Beijing-you chickens. 2. A total of 360 female birds were distributed among 6 treatments, containing 6 replicates, each of 10 birds. The feed for each treatment was supplemented with vitamin E (0, 10, 50, 100, 150, or 200 mg/kg feed). At 120 d, 30 birds from each treatment were slaughtered to examine the effect of dietary vitamin E supplementation on evaluated traits. 3. The results showed that supplemental vitamin E in diet significantly increased alpha-tocopherol contents of breast and thigh muscles, reduced the drip loss and improved tenderness but did not influence carcase yield, meat colour or pH value 24 h after slaughter. 4. Thiobarbituric acid reactive substance (TBARS) values decreased with increase in dietary vitamin E, and the addition of 100 mg/kg or more vitamin E had a beneficial effect on oxidative stability as indicated by TBARS values during storage up to 7 d. 5. Dietary vitamin E supplementation significantly altered fatty acid composition of breast muscle. Supplementing with 200 mg/kg vitamin E led to lower saturated fatty acids and greater polyunsaturated fatty acids proportions in breast muscle than control and 10 mg/kg vitamin E treatments. 6. Vitamin E supplementation significantly inhibited expression of the cytosolic phospholipase A(2) gene (cPLA(2)) in breast muscle, while enhancing that of the peroxisome proliterator-activated receptor beta (PPAP-beta) and heart fatty acid binding protein genes (H-FABP). The results indicate that dietary supplementation with vitamin E increased lipid stability in muscle and improved meat quality and fatty acid composition, probably by its influence on the expression of genes related to lipid metabolism.
Li
W J
WJ
Chinese Academy of Agricultural Sciences, State Key Laboratory of Animal Nutrition, Institute of Animal Science, Beijing, China.
Zhao
G P
GP
Chen
J L
JL
Zheng
M Q
MQ
Wen
J
J
D000818:Animals; D002645:Chickens / Q000378:metabolism*; D004032:Diet; D005227:Fatty Acids / Q000032:analysis; D005260:Female; D015870:Gene Expression / Q000187:drug effects*; D050356:Lipid Metabolism / Q000235:genetics*; D015227:Lipid Peroxidation / Q000187:drug effects; D008055:Lipids / Q000032:analysis; D008460:Meat; D018482:Muscle, Skeletal / Q000737:chemistry; D011786:Quality Control; D012333:RNA, Messenger / Q000032:analysis; D017392:Thiobarbituric Acid Reactive Substances / Q000032:analysis; D014810:Vitamin E / Q000008:administration & dosage*; D024502:alpha-Tocopherol / Q000032:analysis
D005227:Fatty Acids; D008055:Lipids; D012333:RNA, Messenger; D017392:Thiobarbituric Acid Reactive Substances; D014810:Vitamin E; D024502:alpha-Tocopherol