Development and activity of Glomus intraradices as affected by co-existence with Glomus claroideum in one root system.
The co-existence of two arbuscular mycorrhizal fungal (AMF) species, Glomus intraradices and Glomus claroideum, in the root systems of plants was investigated in a greenhouse experiment aimed at reconstructing interactions during an early stage of primary succession on a coal-mine spoil bank in Central Europe. Two plant species, Tripleurospermum inodorum and Calamagrostis epigejos, were inoculated either with one or both AMF species. Fungal development, determined by trypan blue and alkaline phosphatase staining as well as by PCR amplification of rRNA genes with species-specific primers, and the expression of five genes with different metabolic functions in the intraradical structures of G. intraradices were followed after 6 and 9 weeks of cultivation. The two AMF closely co-existed in the root systems of both plants possibly through similar colonisation rates and competitivity. Inoculation with the two fungi, however, did not bring any additional benefit to the host plants in comparison with single inoculation; moreover, plant growth depression observed after inoculation with G. claroideum persisted also in mixed inoculation. The expression of all the assayed G. intraradices genes was affected either by host plant or by co-inoculation with G. claroideum. The effects of both factors depended on the time of sampling, which underlines the importance of addressing this topic in time-course studies.
Janoušková
Martina
M
Institute of Botany, Academy of Sciences of the Czech Republic, 252 43, Průhonice, Czech Republic. janouskova@ibot.cas.cz.
Seddas
Pascale
P
UMR 1088 INRA/5184 CNRS/Université de Bourgogne, Plante-Microbe-Environnement, BP 86510, 21065, Dijon Cedex, France.
Mrnka
Libor
L
Institute of Botany, Academy of Sciences of the Czech Republic, 252 43, Průhonice, Czech Republic.
van Tuinen
Diederik
D
UMR 1088 INRA/5184 CNRS/Université de Bourgogne, Plante-Microbe-Environnement, BP 86510, 21065, Dijon Cedex, France.
Dvořáčková
Anna
A
Institute of Botany, Academy of Sciences of the Czech Republic, 252 43, Průhonice, Czech Republic.
Tollot
Marie
M
UMR 1088 INRA/5184 CNRS/Université de Bourgogne, Plante-Microbe-Environnement, BP 86510, 21065, Dijon Cedex, France.
Gianinazzi-Pearson
Vivienne
V
UMR 1088 INRA/5184 CNRS/Université de Bourgogne, Plante-Microbe-Environnement, BP 86510, 21065, Dijon Cedex, France.
Vosátka
Miroslav
M
Institute of Botany, Academy of Sciences of the Czech Republic, 252 43, Průhonice, Czech Republic.
Gollotte
Armelle
A
UMR 1088 INRA/5184 CNRS/Université de Bourgogne, Plante-Microbe-Environnement, BP 86510, 21065, Dijon Cedex, France.
D000469:Alkaline Phosphatase / Q000378:metabolism; D017931:DNA Primers / Q000235:genetics; D005060:Europe; D020869:Gene Expression Profiling; D005800:Genes, Fungal; D020459:Genes, rRNA; D055137:Glomeromycota / Q000254:growth & development*; D018517:Plant Roots / Q000382:microbiology*; D006109:Poaceae / Q000254:growth & development / Q000382:microbiology*; D016133:Polymerase Chain Reaction / Q000379:methods; D031169:Tripleurospermum / Q000254:growth & development / Q000382:microbiology*; D014343:Trypan Blue / Q000378:metabolism
D017931:DNA Primers; D000469:Alkaline Phosphatase; D014343:Trypan Blue