Multiplexed sandwich immunoassays using electrochemiluminescence imaging resolved at the single bead level.
A new class of bead-based microarray that uses electrogenerated chemiluminescence (ECL) as a readout mechanism to detect multiple antigens simultaneously is presented. This platform demonstrates the possibility of performing highly multiplexed assays using ECL because all the individual sensing beads in the array are simultaneously imaged and individually resolved by ECL. Duplex and triplex assay results are demonstrated as well as a cross reactivity study.
Universite Bordeaux, Institut des Sciences Moleculaires, ENSCPB, 16 Avenue Pey-Berland, 33607 Pessac, France.
Frédérique
F
Deiss
Christopher N
CN
LaFratta
Matthew
M
Symer
Timothy M
TM
Blicharz
Neso
N
Sojic
David R
DR
Walt
D000941:Antigens / Q000032:analysis; D003429:Cross Reactions; D004563:Electrochemistry / Q000379:methods*; D007118:Immunoassay / Q000379:methods*; D008163:Luminescent Measurements / Q000379:methods*; D008863:Microspheres; D040081:Protein Array Analysis / Q000379:methods*
D000941:Antigens