Generation of murine growth factor-dependent long-term dendritic cell lines to investigate host-parasite interactions.
Substantial progress has been made over the last several years in the development of protocols for the isolation of large numbers of dendritic cells (DCs) from different tissues and their short-term culture. Indeed, several stable DC lines and clones have been established from various tissues of mice and humans, providing useful experimental tools for studying the biology of DCs at both molecular and biochemical levels and for the establishment of new DC-based immunotherapies. In this chapter, we will describe the development of long-term DC lines that maintain the growth factor dependence and their immature functional state, thus providing a unique opportunity to study the mechanisms of the initiation of the immune response to infectious agents.
Singapore Immunology Network (SIgN), Agency for Science, Technology and Research (A*STAR), Singapore.
Alessandra
A
Mortellaro
Matteo
M
Urbano
Stefania
S
Citterio
Maria
M
Foti
Francesca
F
Granucci
Paola
P
Ricciardi-Castagnoli
D000818:Animals; D001419:Bacteria / Q000254:growth & development; D001424:Bacterial Infections; D001854:Bone Marrow Cells / Q000166:cytology / Q000187:drug effects; D018929:Cell Culture Techniques / Q000379:methods*; D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D017077:Culture Media, Conditioned; D003713:Dendritic Cells / Q000166:cytology* / Q000187:drug effects / Q000382:microbiology*; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000494:pharmacology*; D006790:Host-Parasite Interactions / Q000187:drug effects*; D051379:Mice; D041681:NIH 3T3 Cells; D010641:Phenotype; D011956:Receptors, Cell Surface / Q000378:metabolism; D013154:Spleen / Q000166:cytology / Q000187:drug effects; D013997:Time Factors
D017077:Culture Media, Conditioned; D011956:Receptors, Cell Surface; D016178:Granulocyte-Macrophage Colony-Stimulating Factor