High-resolution mosaic imaging with multifocal, multiphoton photon-counting microscopy.
High-resolution mosaic imaging is performed for the first time to our knowledge with a multifocal, multiphoton, photon-counting imaging system. We present a novel design consisting of a home-built femtosecond Yb-doped KGdWO(4) laser with an optical multiplexer, which is coupled with a commercial Olympus IX-71 microscope frame. Photon counting is performed using single-element detectors and an inexpensive electronic demultiplexer and counters.
Center for Microintegrated Optics for Advanced Biological Control, Department of Physics, Colorado School of Mines, 1500 Illinois Street, Golden, Colorado 80401, USA. echandle@mines.edu
Eric
E
Chandler
Erich
E
Hoover
Jeff
J
Field
Kraig
K
Sheetz
Wafa
W
Amir
Ramon
R
Carriles
Shi-You
SY
Ding
Jeff
J
Squier
D007834:Lasers; D008853:Microscopy / Q000379:methods*; D017785:Photons