Detection of polyoma and corona viruses in bats of Canada.
Several instances of emerging diseases in humans appear to be caused by the spillover of viruses endemic to bats, either directly or through other animal intermediaries. The objective of this study was to detect, identify and characterize viruses in bats in the province of Manitoba and other regions of Canada. Bats were sampled from three sources: live-trapped Myotis lucifugus from Manitoba, rabies-negative Eptesicus fuscus, M. lucifugus, M. yumanensis, M. septentrionalis, M. californicus, M. evotis, Lasionycteris (L.) noctivagans and Lasiurus (Las.) cinereus, provided by the Centre of Expertise for Rabies of the Canadian Food Inspection Agency (CFIA), and L. noctivagans, Las. cinereus and Las. borealis collected from a wind farm in Manitoba. We attempted to isolate viruses from fresh tissue samples taken from trapped bats in cultured cells of bat, primate, rodent, porcine, ovine and avian origin. We also screened bat tissues by PCR using primers designed to amplify nucleic acids from members of certain families of viruses. We detected RNA of a group 1 coronavirus from M. lucifugus (3 of 31 animals) and DNA from an as-yet undescribed polyomavirus from female M. lucifugus (4 of 31 animals) and M. californicus (pooled tissues from two females).
Misra
Vikram
V
Department of Veterinary Microbiology, Western College of Veterinary Medicine, 52 Campus Road, University of Saskatchewan, Saskatoon, SK S7N 5B4, Canada.
Dumonceaux
Timothy
T
National Microbiology Laboratory, Canadian Science Centre for Human and Animal Disease, 1015 Arlington Street, Winnipeg, MB R3E 3R2, Canada.
Dubois
Jack
J
Manitoba Conservation, Wildlife and Ecosystem Protection Branch, Box 24, 200 Saulteaux Crescent, Winnipeg, MB R3J 3W3, Canada.
Willis
Craig
C
Department of Biology and Centre for Forest Interdisciplinary Research, University of Winnipeg, 515 Portage Avenue, Winnipeg, MB R3B 2E9, Canada.
Nadin-Davis
Susan
S
Centre of Expertise for Rabies, Ottawa Laboratory-Fallowfield, Canadian Food Inspection Agency, 3851 Fallowfield Road, Ottawa, ON K2H 8P9, Canada.
Severini
Alberto
A
National Microbiology Laboratory, Canadian Science Centre for Human and Animal Disease, 1015 Arlington Street, Winnipeg, MB R3E 3R2, Canada.
Wandeler
Alex
A
Centre of Expertise for Rabies, Ottawa Laboratory-Fallowfield, Canadian Food Inspection Agency, 3851 Fallowfield Road, Ottawa, ON K2H 8P9, Canada.
Lindsay
Robbin
R
National Microbiology Laboratory, Canadian Science Centre for Human and Animal Disease, 1015 Arlington Street, Winnipeg, MB R3E 3R2, Canada.
Artsob
Harvey
H
National Microbiology Laboratory, Canadian Science Centre for Human and Animal Disease, 1015 Arlington Street, Winnipeg, MB R3E 3R2, Canada.
D000818:Animals; D002170:Canada; D002478:Cells, Cultured; D002685:Chiroptera / Q000821:virology*; D016000:Cluster Analysis; D017934:Coronavirus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D018352:Coronavirus Infections / Q000662:veterinary* / Q000821:virology; D017931:DNA Primers / Q000235:genetics; D005838:Genotype; D006801:Humans; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods; D011120:Polyomavirus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D027601:Polyomavirus Infections / Q000662:veterinary* / Q000821:virology; D017422:Sequence Analysis, DNA; D014412:Tumor Virus Infections / Q000662:veterinary* / Q000821:virology; D014776:Virus Cultivation / Q000379:methods
D017931:DNA Primers