Biomolecular motor-driven molecular sorter.
We have developed a novel, microfabricated, stand-alone microfluidic device that can efficiently sort and concentrate (bio-)analyte molecules by using kinesin motors and microtubules as a chemo-mechanical transduction machine. The device removes hundreds of targeted molecules per second from an analyte stream by translocating functionalized microtubules with kinesin across the stream and concentrating them at a horseshoe-shaped collector. Target biomolecule concentrations increase up to three orders of magnitude within one hour of operation.
Kim
Taesung
T
Mechanical Engineering, University of Michigan, Ann Arbor, MI, USA.
Cheng
Li-Jing
LJ
Kao
Ming-Tse
MT
Hasselbrink
Ernest F
EF
Guo
Lingjie
L
Meyhöfer
Edgar
E
D001704:Biopolymers / Q000737:chemistry* / Q000302:isolation & purification*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation* / Q000379:methods*; D020409:Molecular Motor Proteins / Q000737:chemistry* / Q000302:isolation & purification*; D025941:Protein Interaction Mapping / Q000295:instrumentation* / Q000379:methods
D001704:Biopolymers; D020409:Molecular Motor Proteins