Tissue-print immunodetection of transgene products in endosperm for high-throughput screening of seeds.
This method allows high-throughput qualitative screening to identify seeds containing a transgene product in endosperm tissue. It is particularly useful for determining genetic segregation ratios or identifying seeds to be advanced in a breeding program. Tissue printing is used to avoid time-consuming extraction steps. Antibody-based detection of the transgene product makes this method suitable to any transgene product for which a specific antibody is available. It is possible to screen thousands of seeds per week using this method.
Scott
M Paul
MP
Corn Insects and Crop Genetics Research Unit, USDA-ARS, Ames, IA, USA. Paul.scott@ars.usda.gov
D001947:Breeding; D015870:Gene Expression; D005821:Genetic Techniques; D015151:Immunoblotting / Q000379:methods*; D030821:Plants, Genetically Modified; D012639:Seeds / Q000235:genetics; D003313:Zea mays / Q000196:embryology / Q000235:genetics*