Variation in the levels of inflammatory cytokines depending on ischemic time: effects on respiratory variables.
OBJECTIVE
We sought to evaluate the association between ischemic times, cytokines-interleukin (IL)-6, IL-1b, tumor necrosis factor-alpha, sIL-2r, IL-8, and IL-10-and alterations in gaseous exchange.


METHODS
This prospective study of 42 orthotopic liver transplantation (OLT) recipients examined ischemic times and respiratory variables measured as alterations in intrapulmonary shunt and in the Po(2)/Fio(2) ratio. Centrifuged blood samples were frozen at -80 degrees C for storage. The Inmulite-One system (Euro/Dpc, Gwynedd, UK) was used to determine the concentration of cytokines. For statistical analysis, we used the Pearson correlation coefficient.


RESULTS
The average cold ischemic time was 478 minutes (range, 35-929) and warm ischemic time was 69.58 minutes (range, 20-180). The warm ischemic time affected the degree of shunt at the end of the operation (P < .027) and the levels of IL-10 (P < .018) and IL-6 (P < .000). The final degree of shunting and IL-10 (P < .044) showed a correlation. The cold ischemic time affected IL-1 (P < .046) and IL-8 levels (P < .023). The reperfusion syndrome was correlated with the final levels of IL-10 (P < .064) and of IL-8 (P < .066).


CONCLUSIONS
Warm and cold ischemic times affect the final cytokine levels and the degree of intrapulmonary shunt.
Liver Transplant Unit, Hospital Universitario NS de Candelaria, Tenerife, Spain. javierarranz@telefonica.net
J Arranz
JA
Duran
A Arteaga
AA
González
D Domínguez
DD
García
R Calvo
RC
Falcón
P Sanz
PS
Pereda
S Méndez
SM
Alvárez
I García
IG
Cobaleda
A Aguirre
AA
Jaime
I Arteaga
IA
González
A Varona
AV
Bosque
M Barrera
MB
Gómez
A Soriano
AS
Benítez de Lugo
D016207:Cytokines / Q000097:blood*; D006801:Humans; D007249:Inflammation / Q000097:blood; D007375:Interleukin-1 / Q000097:blood; D016753:Interleukin-10 / Q000097:blood; D015850:Interleukin-6 / Q000097:blood; D016209:Interleukin-8 / Q000097:blood; D007511:Ischemia / Q000097:blood / Q000276:immunology*; D008102:Liver Circulation / Q000276:immunology; D016031:Liver Transplantation / Q000276:immunology*; D010100:Oxygen / Q000097:blood; D010101:Oxygen Consumption; D010313:Partial Pressure; D011169:Portal Vein / Q000502:physiology; D015424:Reperfusion; D015427:Reperfusion Injury / Q000097:blood / Q000276:immunology*
D016207:Cytokines; D007375:Interleukin-1; D015850:Interleukin-6; D016209:Interleukin-8; D016753:Interleukin-10; D010100:Oxygen