Label free optical sensor for Avidin based on single gold nanoparticles functionalized with aptamers.
Optical spectroscopy of a single gold nanoparticle, functionalized with an aptamer, is used to sense the specific binding of avidin. Herewith, the field of single noble metal nanoparticle biosensors is extended to the important field of aptamer based assays. The sensitivity of this initial, but not yet optimized apta-nano-sensor is in the range of 20 nM. Due to its nanoscopic size, this single nanoparticle based apta-sensor may be used in nanoscopic volumes such as in array type assays or even inside cells.
Hernandez
Frank Jeyson
FJ
Department of Chemical Engineering, Universitat Rovira i Virgili, Tarragona, Spain.
Dondapati
Srujan Kumar
SK
Ozalp
V Cengiz
VC
Pinto
Alessandro
A
O'Sullivan
Ciara K
CK
Klar
Thomas A
TA
Katakis
Ioannis
I
D052157:Aptamers, Nucleotide / Q000378:metabolism*; D001360:Avidin / Q000032:analysis* / Q000378:metabolism; D015374:Biosensing Techniques / Q000379:methods*; D006046:Gold / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry*; D055620:Optical Phenomena; D012680:Sensitivity and Specificity; D013057:Spectrum Analysis; D013194:Staining and Labeling
D052157:Aptamers, Nucleotide; D001360:Avidin; D006046:Gold