Potential hepatoprotective activity of ononitol monohydrate isolated from Cassia tora L. on carbon tetrachloride induced hepatotoxicity in wistar rats.
Ononitol monohydrate, structurally similar to glycoside was isolated from Cassia tora L. leaves. Fifty Male rats were divided into five groups. Group I served as normal control. Group II, III and IV rats were induced hepatotoxicity by CCl(4) administering single dose of CCl(4) on 8th day only. Group III was treated with ononitol monohydrate (20mg/kg body weight) and group IV was treated with reference drug silymarin (20mg/kg body weight) both dissolved in corn oil and administering for 8 days. Ononitol monohydrate with corn oil alone was given for 8 days (group V). At the end of the experimental period all the animals were sacrificed and analyzed for biochemical parameters to assess the effect of ononitol monohydrate treatment in CCl(4) induced hepatotoxicity. In in vivo study, ononitol monohydrate decreased the levels of serum transaminase, lipid peroxidation and TNF-alpha but increased the levels of antioxidant and hepatic glutathione enzyme activities. Compared with reference drug silymarin ononitol monohydrate possessed high hepatoprotective activity. Histopathological results also suggested the hepatoprotective activity of ononitol monohydrate with no adverse effect. Hence we conclude that ononitol monohydrate is a potent hepatoprotective agent.
Dhanasekaran
Muniyappan
M
Division of Ethnopharmacology, Entomology Research Institute, Loyola College, Tamil Nadu, India.
Ignacimuthu
Savarimuthu
S
Agastian
Paul
P
D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D002251:Carbon Tetrachloride; D002366:Cassia / Q000737:chemistry*; D056486:Chemical and Drug Induced Liver Injury / Q000517:prevention & control*; D005978:Glutathione / Q000378:metabolism; D006027:Glycosides / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use*; D015227:Lipid Peroxidation / Q000187:drug effects; D008099:Liver / Q000187:drug effects* / Q000473:pathology; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D008517:Phytotherapy; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use*; D018515:Plant Leaves; D020011:Protective Agents / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use*; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D012838:Silymarin / Q000494:pharmacology / Q000627:therapeutic use; D000637:Transaminases / Q000097:blood; D014409:Tumor Necrosis Factor-alpha / Q000097:blood
D000975:Antioxidants; D006027:Glycosides; D010936:Plant Extracts; D020011:Protective Agents; D012838:Silymarin; D014409:Tumor Necrosis Factor-alpha; C544115:ononitol monohydrate; D008315:Malondialdehyde; D002251:Carbon Tetrachloride; D000637:Transaminases; D005978:Glutathione