In vivo measurement of local aortic pulse-wave velocity in mice with MR microscopy at 17.6 Tesla.
Transgenic mouse models of human diseases have gained increasing importance in the pathophysiology of cardiovascular diseases (CVD). As an indirect measure of vascular stiffness, aortic pulse-wave velocity (PWV) is an important predictor of cardiovascular risk. This study presents an MRI approach that uses a flow area method to estimate local aortic pulse-wave velocity at different sites in the murine aorta. By simultaneously measuring the cross-sectional area and the through-plane velocity with a phase-contrast CINE method, it was possible to measure average values for the PWV in the ascending and descending aorta within the range of 2.4-4.3 m/s for C57BL/6J mice (ages 2 and 8 months) and apoE-knockout mice (age 8 months). Statistically significant differences of the mean values of the PWV of both groups could be determined. By repeating CINE measurements with a time delay of 1 ms between two subsequent data sets, an effective temporal resolution of 1000 frames/s (fps) could be achieved.
Herold
Volker
V
Julius-Maximilians-Universität Würzburg, Lehrstuhl für Experimentelle Physik 5, Würzburg, Germany. vrherold@physik.uni-wuerzburg.de
Parczyk
Marco
M
Mörchel
Philipp
P
Ziener
Christian H
CH
Klug
Gert
G
Bauer
Wolfgang R
WR
Rommel
Eberhard
E
Jakob
Peter M
PM
D000818:Animals; D001011:Aorta / Q000033:anatomy & histology / Q000502:physiology*; D001057:Apolipoproteins E / Q000235:genetics*; D001783:Blood Flow Velocity / Q000502:physiology*; D018810:Magnetic Resonance Angiography / Q000379:methods* / Q000662:veterinary; D019028:Magnetic Resonance Imaging, Cine / Q000379:methods*; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D008822:Mice, Transgenic; D008853:Microscopy / Q000379:methods* / Q000662:veterinary; D011673:Pulsatile Flow / Q000502:physiology*
D001057:Apolipoproteins E