Gold-silver nanoclusters having dipicolinic acid imprinted nanoshell for Bacillus cereus spores recognition.
Molecular imprinted polymers (MIPs) as a recognition element for sensors are increasingly of interest and MIP nanoclusters have started to appear in the literature. In this study, we have proposed a novel thiol ligand-capping method with polymerizable methacryloylamidocysteine (MAC) attached to gold-silver nanoclusters, reminiscent of a self-assembled monolayer and have reconstructed surface shell by synthetic host polymers based on molecular imprinting method for recognition. In this method, methacryloyl iminodiacetic acid-chrome (MAIDA-Cr(III)) has been used as a new metal-chelating monomer via metal coordination-chelation interactions and dipicolinic acid (DPA) which is a main participant of Bacillus spores has been used as a template. Nanoshell sensors with templates give a cavity that is selective for DPA. The DPA can simultaneously chelate to Cr(III) metal ion and fit into the shape-selective cavity. Thus, the interaction between Cr(III) ion and free coordination spheres has an effect on the binding ability of the gold-silver nanoclusters nanosensor. The binding affinity of the DPA imprinted nanoclusters has been investigated by using the Langmuir and Scatchard methods and determined affinity constants (K(affinity)) were found as 18 x 10(6) mol L(-1) and 9 x 10(6) mol L(-1), respectively.
Gültekin
Aytaç
A
Department of Chemistry, Trakya University, Edirne, Turkey.
Diltemiz
S Emir
SE
Ersöz
Arzu
A
Sariözlü
N Yilmaz
NY
Denizli
Adil
A
Say
Ridvan
R
D001409:Bacillus cereus / Q000166:cytology*; D001431:Bacteriological Techniques; D002614:Chelating Agents; D006046:Gold; D053768:Metal Nanoparticles / Q000737:chemistry*; D054802:Molecular Imprinting / Q000379:methods*; D010848:Picolinic Acids / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry; D012834:Silver; D013171:Spores, Bacterial / Q000302:isolation & purification*
D002614:Chelating Agents; D010848:Picolinic Acids; D011108:Polymers; D012834:Silver; D006046:Gold; C004860:dipicolinic acid