Construction of cell-type specific promoter lentiviruses for optically guiding electrophysiological recordings and for targeted gene delivery.
It is often advantageous to identify and alter gene expression of specific cell populations within the brain. Currently, it is not possible to a priori identify specific cell types within the brain of rats for electrophysiological recordings, nor is it possible to routinely alter gene expression in specific cell types within the CNS of a variety of species. Here, we describe a general method for the relatively rapid screening of specific promoter activity in cell culture, in acute brain slice preparations, and in vivo. As an example, we describe the examination of an approximately 3 kb promoter region of the neuroactive peptide cholecystokinin (CCK) compared to the ubiquitous cytomegalovirus (CMV) promoter. We find a high degree of cell-type specificity in vivo using lentiviral approaches in rats and mice.
Jasnow
Aaron M
AM
Yerkes Primate Research Center, Emory University, Atlanta, GA 30329, USA. ajasnow@emory.edu
Rainnie
Donald G
DG
Maguschak
Kimberly A
KA
Chhatwal
Jasmeer P
JP
Ressler
Kerry J
KJ
D002460:Cell Line; D055724:Electrophysiological Phenomena; D006801:Humans; D016086:Lentivirus / Q000235:genetics*; D010957:Plasmids / Q000235:genetics; D011401:Promoter Regions, Genetic / Q000235:genetics*; D017186:Titrimetry; D014162:Transfection / Q000379:methods*