Development of medium for enhanced production of glutaminase-free L-asparaginase from Pectobacterium carotovorum MTCC 1428.
Glutaminase-free L-asparaginase is known to be an excellent anticancer agent. In the present study, statistically based experimental designs were applied to maximize the production of glutaminase-free L-asparaginase from Pectobacterium carotovorum MTCC 1428. Nine components of the medium were examined for their significance on the production of L-asparaginase using the Plackett-Burman experimental design. The medium components, viz., glucose, L-asparagine, KH2PO4, and MgSO(4).7H2O, were screened based on their high confidence levels (P<0.04). The optimum levels of glucose, L-asparagine, KH2PO4, and MgSO(4).7H2O were found to be 2.076, 5.202, 1.773, and 0.373 g L(-1), respectively, using the central composite experimental design. The maximum specific activity of L-asparaginase in the optimized medium was 27.88 U mg(-1) of protein, resulting in an overall 8.3-fold increase in the production compared to the unoptimized medium.
Biochemical Engineering Laboratory, Department of Biotechnology, Indian Institute of Technology Guwahati, Guwahati, Assam, 781039, India.
Sanjay
S
Kumar
K
K
Pakshirajan
V
V
Venkata Dasu
D000970:Antineoplastic Agents / Q000378:metabolism; D001215:Asparaginase / Q000096:biosynthesis* / Q000737:chemistry; D001216:Asparagine / Q000378:metabolism; D001709:Biotechnology / Q000379:methods*; D003470:Culture Media / Q000737:chemistry*; D005947:Glucose / Q000378:metabolism; D005972:Glutaminase / Q000032:analysis; D015233:Models, Statistical; D016973:Pectobacterium carotovorum / Q000201:enzymology* / Q000254:growth & development
D000970:Antineoplastic Agents; D003470:Culture Media; D001216:Asparagine; D001215:Asparaginase; D005972:Glutaminase; D005947:Glucose