Label free optical sensor for Avidin based on single gold nanoparticles functionalized with aptamers.
Optical spectroscopy of a single gold nanoparticle, functionalized with an aptamer, is used to sense the specific binding of avidin. Herewith, the field of single noble metal nanoparticle biosensors is extended to the important field of aptamer based assays. The sensitivity of this initial, but not yet optimized apta-nano-sensor is in the range of 20 nM. Due to its nanoscopic size, this single nanoparticle based apta-sensor may be used in nanoscopic volumes such as in array type assays or even inside cells.
Department of Chemical Engineering, Universitat Rovira i Virgili, Tarragona, Spain.
Frank Jeyson
FJ
Hernandez
Srujan Kumar
SK
Dondapati
V Cengiz
VC
Ozalp
Alessandro
A
Pinto
Ciara K
CK
O'Sullivan
Thomas A
TA
Klar
Ioannis
I
Katakis
D052157:Aptamers, Nucleotide / Q000378:metabolism*; D001360:Avidin / Q000032:analysis* / Q000378:metabolism; D015374:Biosensing Techniques / Q000379:methods*; D006046:Gold / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry*; D055620:Optical Phenomena; D012680:Sensitivity and Specificity; D013057:Spectrum Analysis; D013194:Staining and Labeling
D052157:Aptamers, Nucleotide; D001360:Avidin; D006046:Gold