Biofouling of dextran-derivative layers investigated by quartz crystal microbalance.
This study reports the fouling of carboxymethyl dextran (CMD) layers in cell culture medium, fibronectin, and serum solutions. CMD layers were covalently immobilized onto amine groups available either on an n-heptylamine plasma polymer (HApp) layer or onto a polyethylenimine (PEI) coating grafted to an acetaldehyde plasma polymer (AApp) layer. The successful immobilization of the graft layers was demonstrated by X-ray photoelectron spectroscopy (XPS). Primary amines on HApp and AApp+PEI surfaces were quantified using a colorimetric assay. Quartz crystal microbalance (QCM) was used to investigate in real-time the fouling of the graft layers upon incubation in cell culture medium (RPMI), fibronectin, and foetal bovine serum (FBS) solutions. HApp, AApp and AApp+PEI layers exhibited large fouling in fibronectin and FBS solutions, while fouling in RPMI cell culture medium was not significant. Protein repellent properties of CMD layers in FBS and fibronectin have been demonstrated compared to the other tested surfaces. QCM has shown that both CMD layers were fouled to a small extent in RPMI medium.
Laboratoire de Bioingénierie et de Biophysique de l'Université de Sherbrooke, Department of Chemical and Biotechnological Engineering, Université de Sherbrooke, 2500, blvd de l'Université, Sherbrooke, Québec, Canada J1K 2R1.
Justin
J
Dubois
Charles
C
Gaudreault
Patrick
P
Vermette
D000327:Adsorption; D000704:Analysis of Variance; D000818:Animals; D002417:Cattle; D003460:Crystallization; D003470:Culture Media / Q000494:pharmacology*; D003911:Dextrans / Q000737:chemistry* / Q000378:metabolism*; D005353:Fibronectins / Q000378:metabolism; D011094:Polyethyleneimine / Q000737:chemistry; D011791:Quartz / Q000737:chemistry*; D013057:Spectrum Analysis; D013499:Surface Properties
D003470:Culture Media; D003911:Dextrans; D005353:Fibronectins; D011791:Quartz; D011094:Polyethyleneimine