An ultramarine fluorescent protein with increased photostability and pH insensitivity.
We report a pH-insensitive and photostable ultramarine fluorescent protein, Sirius, with an emission peak at 424 nm, the shortest emission wavelength among fluorescent proteins reported to date. The pH-insensitivity of Sirius allowed prolonged visualization of biological events in an acidic environment. Two fluorescence resonance energy transfer (FRET) pairs, Sirius-mseCFP and Sapphire-DsRed, allowed dual-FRET imaging with single-wavelength excitation, enabling detection of Ca(2+) concentration and caspase-3 activation in the same apoptotic cells.
Laboratory for Nanosystems Physiology, Research Institute for Electronic Science, Hokkaido University, Sapporo, Hokkaido, Japan.
Wataru
W
Tomosugi
Tomoki
T
Matsuda
Tomomi
T
Tani
Tomomi
T
Nemoto
Ippei
I
Kotera
Kenta
K
Saito
Kazuki
K
Horikawa
Takeharu
T
Nagai
D019943:Amino Acid Substitution; D000818:Animals; D017209:Apoptosis; D020013:Calcium Signaling; D053148:Caspase 3 / Q000378:metabolism; D003116:Color; D004023:Dictyostelium / Q000382:microbiology; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D031541:Fluorescence Resonance Energy Transfer; D049452:Green Fluorescent Proteins / Q000737:chemistry / Q000235:genetics; D006367:HeLa Cells; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008164:Luminescent Proteins / Q000737:chemistry* / Q000235:genetics; D008969:Molecular Sequence Data; D016296:Mutagenesis; D010587:Phagocytosis; D038761:Photobleaching; D055550:Protein Stability; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D014162:Transfection
C485182:Cyan Fluorescent Protein; D008164:Luminescent Proteins; D011993:Recombinant Fusion Proteins; C409504:blue fluorescent protein, Aequorea victoria; C413663:fluorescent protein 583; C485192:green fluorescent protein, Aequorea victoria; D049452:Green Fluorescent Proteins; D053148:Caspase 3