Discovering protein kinase C active plants growing in Finland utilizing automated bioassay combined to LC/MS.
Protein kinase C (PKC) modulating activity of 81 plant extracts of Finnish origin was investigated with an automated bioassay method combined to LC/MS. Twenty-one extracts from different parts of the plants inhibited PKC significantly. Fractionation of the active extract of Filipendula ulmaria showed that this method was able to identify a PKC inhibiting compound from the extract as quercetin. Our results indicate that this method is suitable for PKC screening of complex matrices and provides a quick and low volume, non-radioactive, alternative method for PKC experiments.
Galkin
Anna
A
Center for Drug Research and Division of Pharmaceutical Biology, Faculty of Pharmacy, FIN-00014 University of Helsinki, Finland.
Jokela
Jouni
J
Wahlsten
Matti
M
Tammela
Päivi
P
Sivonen
Kaarina
K
Vuorela
Pia
P
D001331:Automation; D001681:Biological Assay; D002853:Chromatography, Liquid; D005387:Finland; D013058:Mass Spectrometry; D010936:Plant Extracts / Q000737:chemistry* / Q000494:pharmacology*; D010944:Plants / Q000737:chemistry*; D011493:Protein Kinase C / Q000037:antagonists & inhibitors* / Q000737:chemistry*
D010936:Plant Extracts; D011493:Protein Kinase C