Expression and localization of endothelin-1 and its receptors in the spiral ganglion neurons of mouse.
Endothelin-1 (ET-1) is a peptide with various biological functions, such as vasoconstriction and cell proliferation. ET-1 was reported to be widely distributed throughout the animal body, including nervous system. The expression and localization of ET-1 and its receptors [endothelin type-A receptor (ETAR) and endothelin type-B receptor (ETBR)] in the spiral ganglion neurons have not been reported before. In this study, their presence in the mouse spiral ganglion neurons was detected at mRNA and protein levels by reverse transcription-polymerase chain reaction (RT-PCR) technique and immunohistochemistry, respectively. RT-PCR analysis indicated that ET-1, ETAR, and ETBR genes were expressed in the mouse spiral ganglion tissues. Immunohistochemical experiments demonstrated that ET-1 and ETAR were predominantly immunoreactive in the cytoplasm, while ETBR was mainly immunostained in the nucleus of the neuron bodies. The present results suggest that ET-1 may play physiological roles in the spiral ganglion cells via ETAR and ETBR.
Department of Otorhinolaryngology, West China Hospital of Sichuan University, No. 37 Guo-Xue-Xiang, 610041, Chengdu, Sichuan, People's Republic of China.
Tao
T
Liu
Lili
L
Long
Tian
T
Tang
Qingjie
Q
Xia
Jin
J
Liu
Gang
G
He
Xiaoming
X
Qiao
D000818:Animals; D019332:Endothelin-1 / Q000378:metabolism*; D007150:Immunohistochemistry; D051379:Mice; D009474:Neurons / Q000166:cytology / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D021381:Protein Transport; D044022:Receptor, Endothelin A / Q000378:metabolism*; D044023:Receptor, Endothelin B / Q000378:metabolism*; D013136:Spiral Ganglion / Q000166:cytology / Q000378:metabolism*
D019332:Endothelin-1; D044022:Receptor, Endothelin A; D044023:Receptor, Endothelin B