A facile system for encoding unnatural amino acids in mammalian cells.
A shuttle system has been developed to genetically encode unnatural amino acids in mammalian cells using aminoacyl-tRNA synthetases (aaRSs) evolved in E. coli. A pyrrolysyl-tRNA synthetase (PylRS) mutant was evolved in E. coli that selectively aminoacylates a cognate nonsense suppressor tRNA with a photocaged lysine derivative. Transfer of this orthogonal tRNA-aaRS pair into mammalian cells made possible the selective incorporation of this unnatural amino acid into proteins.
Department of Chemistry, The Scripps Research Institute, 10550 Torry Pines Road, La Jolla, CA 92037, USA.
Peng R
PR
Chen
Dan
D
Groff
Jiantao
J
Guo
Weijia
W
Ou
Susan
S
Cellitti
Bernhard H
BH
Geierstanger
Peter G
PG
Schultz
D000596:Amino Acids / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics*; D000604:Amino Acyl-tRNA Synthetases / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D001105:Archaea / Q000235:genetics; D016466:CHO Cells; D002460:Cell Line; D006224:Cricetinae; D003412:Cricetulus; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D005815:Genetic Code; D006801:Humans; D008239:Lysine / Q000031:analogs & derivatives* / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D014176:Protein Biosynthesis; D011506:Proteins / Q000737:chemistry* / Q000235:genetics
D000596:Amino Acids; D011506:Proteins; D000604:Amino Acyl-tRNA Synthetases; C456839:pyrrolysine; D008239:Lysine