Separation and characterization of lipopolysaccharide related compounds by HPLC/post-column fluorescence derivatization (HPLC/FLD) and capillary zone electrophoresis/mass spectrometry (CZE/MS).
The O,N-deacylated derivative (deON) and polysaccharide part (PS) from the lipopolysaccharide (LPS) of Escherichia coli C strain were separated by strongly basic anion-exchange chromatography (SAX) based on the differences in the number of charged phosphate and ethanolamine substituents. They were also successfully separated and characterized by capillary zone electrophoresis and subsequent ESI-ion trap-MS (CZE/ESI-IT-MS). The O-deacylated LPS (deO) presented as a broad peak in CZE/ESI-IT-MS. However, more than twelve species could be discriminated by an extracted ion electropherogram (EIE) and monitoring the species which have different numbers of phosphate and ethanolamine substituents on polysaccharide backbone.
Analytical Science, PreClinical Development, Banyu Pharmaceutical Co Ltd, Tsukuba, Ibaraki, Japan.
Hisaki
H
Kojima
Minoru
M
Inagaki
Tsuyoshi
T
Tomita
Teruko
T
Watanabe
Satoko
S
Uchida
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D019075:Electrophoresis, Capillary / Q000379:methods*; D004926:Escherichia coli / Q000737:chemistry*; D008070:Lipopolysaccharides / Q000737:chemistry*; D013058:Mass Spectrometry / Q000379:methods*
D008070:Lipopolysaccharides