[Changes in the expression of salivary gland genes in Ixodes persulcatus (Ixodidae) depending on the stage of tick feeding].
By using the guanidine-isothiocyanate test, the authors isolated a summary RNA preparation from Ixodes persulcatus salivary gland extracts. Activity products of the genes responsible for the expression of some salivary proteins were first identified using the RT-PCR. It has been shown that, firstly, I. persulcatus synthesizes at least 3 transcripts homologous to the respective salivary components of the related species I. scapularis, the translation product of which is likely to be immunodominant antigens; secondly, the number of each of these transcripts, as in I. scapularis, depends on the stage of tick feeding. The changes in the expression of each transcript are specific: monotonously increasing changes in Salp 17 and cyclic ones in Salp 16, and synthesis, only when the ticks are fully ingested, in Salp 25.
Shtannikov
A V
AV
Perovskaia
O N
ON
Reshetniak
T V
TV
Repolovskaia
T V
TV
Panfertsev
E A
EA
Sergeeva
E E
EE
Gutova
V P
VP
Vasil'eva
I S
IS
Ershova
A S
AS
Prilipov
A G
AG
Biketov
S F
SF
Zeidner
N
N
D000818:Animals; D005247:Feeding Behavior; D005260:Female; D005786:Gene Expression Regulation; D018884:Ixodes / Q000235:genetics* / Q000502:physiology; D012333:RNA, Messenger / Q000032:analysis / Q000096:biosynthesis; D011817:Rabbits; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012469:Salivary Glands / Q000378:metabolism*; D012471:Salivary Proteins and Peptides / Q000235:genetics*
D012333:RNA, Messenger; D012471:Salivary Proteins and Peptides