Insights into the enzymatic mechanism of 6-phosphogluconolactonase from Trypanosoma brucei using structural data and molecular dynamics simulation.
Trypanosoma brucei is the causative agent of African sleeping sickness. Current work for the development of new drugs against this pathology includes evaluation of enzymes of the pentose phosphate pathway (PPP), which first requires a clear understanding of their function and mechanism of action. In this context, we focused on T. brucei 6-phosphogluconolactonase (Tb6PGL), which converts delta-6-phosphogluconolactone into 6-phosphogluconic acid in the second step of the PPP. We have determined the crystal structure of Tb6PGL in complex with two ligands, 6-phosphogluconic acid and citrate, at 2.2 A and 2.0 A resolution, respectively. We have performed molecular dynamics (MD) simulations on Tb6PGL in its empty form and in complex with delta-6-phosphogluconolactone, its natural ligand. Analysis of the structural data and MD simulations allowed us to propose a detailed enzymatic mechanism for 6PGL enzymes.
Unité de Bioinformatique Structurale, Institut Pasteur, URA 2185 du CNRS, Paris, France.
Nathalie
N
Duclert-Savatier
Luisa
L
Poggi
Emeric
E
Miclet
Philippe
P
Lopes
Jamal
J
Ouazzani
Nathalie
N
Chevalier
Michael
M
Nilges
Marc
M
Delarue
Véronique
V
Stoven
D000818:Animals; D002265:Carboxylic Ester Hydrolases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D019343:Citric Acid / Q000737:chemistry / Q000378:metabolism; D003198:Computer Simulation; D018360:Crystallography, X-Ray; D005942:Gluconates / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D008958:Models, Molecular; D008969:Molecular Sequence Data; D015394:Molecular Structure; D014346:Trypanosoma brucei brucei / Q000201:enzymology*; D014353:Trypanosomiasis, African; D014961:X-Ray Diffraction
C114004:6-phosphogluconolactone; D005942:Gluconates; D019343:Citric Acid; D002265:Carboxylic Ester Hydrolases; C022363:6-phosphogluconolactonase