Lymphoid-tissue-specific homing of bone-marrow-derived dendritic cells.
Because of their potent immunoregulatory capacity, dendritic cells (DCs) have been exploited as therapeutic tools to boost immune responses against tumors or pathogens, or dampen autoimmune or allergic responses. Murine bone marrow-derived DCs (BM-DCs) are the closest known equivalent of the blood monocyte-derived DCs that have been used for human therapy. Current imaging methods have proven unable to properly address the migration of injected DCs to small and deep tissues in mice and humans. This study presents the first extensive analysis of BM-DC homing to lymph nodes (and other selected tissues) after intravenous and intraperitoneal inoculation. After intravenous delivery, DCs accumulated in the spleen, and preferentially in the pancreatic and lung-draining lymph nodes. In contrast, DCs injected intraperitoneally were found predominantly in peritoneal lymph nodes (pancreatic in particular), and in omentum-associated lymphoid tissue. This uneven distribution of BM-DCs, independent of the mouse strain and also observed within pancreatic lymph nodes, resulted in the uneven induction of immune response in different lymphoid tissues. These data have important implications for the design of systemic cellular therapy with DCs, and in particular underlie a previously unsuspected potential for specific treatment of diseases such as autoimmune diabetes and pancreatic cancer.
Department of Medicine, Division of Immunology and Rheumatology, Stanford University School of Medicine, 300 Pasteur Drive, Stanford, CA 94305, USA.
Rémi J
RJ
Creusot
Shahriar S
SS
Yaghoubi
Pearl
P
Chang
Justine
J
Chia
Christopher H
CH
Contag
Sanjiv S
SS
Gambhir
C Garrison
CG
Fathman
D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology; D002465:Cell Movement / Q000502:physiology; D003713:Dendritic Cells / Q000166:cytology* / Q000637:transplantation; D005260:Female; D017930:Genes, Reporter; D016219:Immunotherapy, Adoptive; D007274:Injections, Intraperitoneal; D007275:Injections, Intravenous; D049409:Luciferases, Firefly / Q000032:analysis / Q000235:genetics; D008168:Lung; D008198:Lymph Nodes / Q000166:cytology; D008221:Lymphoid Tissue / Q000166:cytology*; D051379:Mice; D008807:Mice, Inbred BALB C; D016688:Mice, Inbred NOD; D008822:Mice, Transgenic; D009852:Omentum; D009928:Organ Specificity; D010179:Pancreas; D013154:Spleen
D049409:Luciferases, Firefly