Functional role of GABAergic and glycinergic inhibition in the intermediate nucleus of the lateral lemniscus of the big brown bat.
The intermediate nucleus of the lateral lemniscus (INLL) is a major input to the inferior colliculus (IC), the auditory midbrain center where multiple pathways converge to create neurons selective for specific temporal features of sound. However, little is known about how INLL processes auditory information or how it contributes to integrative processes at the IC. INLL receives excitatory projections from the cochlear nucleus and inhibitory projections from the medial nucleus of the trapezoid body (MNTB), so it must perform some form of integration. To address the question of what role inhibitory synaptic inputs play in the INLL of the big brown bat (Eptesicus fuscus), we recorded sound-evoked responses of single neurons and iontophoretically applied bicuculline to block GABA(A) receptors or strychnine to block glycine receptors. Neither bicuculline nor strychnine had a consistent effect on response latency or frequency response areas. Bicuculline increased spike counts and response durations in most units, suggesting that GABAergic input suppressed the late part of the response and provided some gain control. Strychnine reduced the responses of some units with sustained discharge patterns to one or a few spikes at stimulus onset, but increased others. INLL is the only part of the auditory system where reduced responsiveness has been seen in vivo while blocking glycine. However, in vitro studies in the MNTB suggest that glycine can be facilitatory, possibly through presynaptic action. These results show that GABA consistently reduces spike counts and response durations, whereas glycine is suppressive in some INLL neurons but facilitatory in others.
Department of Psychology, University of Washington, Seattle, Washington 98195-1525, USA.
Andrew
A
Kutscher
Ellen
E
Covey
D000161:Acoustic Stimulation / Q000379:methods; D000818:Animals; D001306:Auditory Pathways / Q000502:physiology; D001640:Bicuculline / Q000494:pharmacology; D001931:Brain Mapping; D002685:Chiroptera / Q000502:physiology*; D018756:GABA Antagonists / Q000494:pharmacology; D005998:Glycine / Q000378:metabolism*; D018684:Glycine Agents / Q000494:pharmacology; D007245:Inferior Colliculi / Q000502:physiology*; D007478:Iontophoresis / Q000379:methods; D009433:Neural Inhibition / Q000502:physiology*; D011571:Psychoacoustics; D011930:Reaction Time / Q000502:physiology; D011984:Sensory Receptor Cells / Q000502:physiology*; D013331:Strychnine / Q000494:pharmacology; D005680:gamma-Aminobutyric Acid / Q000378:metabolism*
D018756:GABA Antagonists; D018684:Glycine Agents; D005680:gamma-Aminobutyric Acid; D013331:Strychnine; D005998:Glycine; D001640:Bicuculline