Transformation of Arabidopsis with plant-derived DNA sequences necessary for selecting transformants and driving an objective gene.
Most genetically modified plants are made with antibiotic-resistant genes and promoters derived from plant pathogens. To promote public acceptance, we have adapted a selectable marker and promoters all of which were derived from Arabidopsis thaliana to transformation. We have employed the gene for acetolactate synthase with its own promoter as a selectable marker, and the actin 2 gene promoter to strongly express an objective gene.
Ahmad
Aftab
A
Laboratory of Plant Molecular Improvement and Global COE Program, Graduate School of Nutritional and Environmental Science, University of Shizuoka, 52-1 Yada, Suruga, Shizuoka, Shizuoka 422-8526, Japan.
Kaji
Izumi
I
Murakami
Yukiko
Y
Funato
Nana
N
Ogawa
Takeshi
T
Shimizu
Masanori
M
Niwa
Yasuo
Y
Kobayashi
Hirokazu
H
D017360:Arabidopsis / Q000235:genetics*; D001483:Base Sequence; D015415:Biomarkers; D018744:DNA, Plant / Q000235:genetics*; D017343:Genes, Plant; D030821:Plants, Genetically Modified; D014170:Transformation, Genetic
D015415:Biomarkers; D018744:DNA, Plant