The decontaminative effects of acidic electrolyzed water for Escherichia coli O157:H7, Salmonella typhimurium, and Listeria monocytogenes on green onions and tomatoes with differing organic demands.
Acidic electrolyzed water (AC-EW) has strong bactericidal activity against foodborne pathogens on fresh vegetables. However, the efficacy of AC-EW is influenced by soil or other organic materials present. This study examined the bactericidal activity of AC-EW in the presence of organic matter, in the form of bovine serum against foodborne pathogens on the surfaces of green onions and tomatoes. Green onions and tomatoes were inoculated with a culture cocktail of Escherichia coli O157:H7, Salmonella typhimurium, and Listeria monocytogenes. Treatment of these organisms with AC-EW containing bovine serum concentrations of 5, 10, 15, and 20 ml/l was performed for 15s, 30s, 1 min, 3 min and 5 min. The total residual chlorine concentrations of AC-EW decreased proportional to the addition of serum. The bactericidal activity of AC-EW also decreased with increasing bovine serum concentration, whereas unamended AC-EW treatment reduced levels of cells to below the detection limit (0.7 logCFU/g) within 3 min.
Park
Eun-Jin
EJ
School of Food Science, Washington State University, Pullman, WA 99164-6376, USA.
Alexander
Edward
E
Taylor
Gary A
GA
Costa
Roy
R
Kang
Dong-Hyun
DH
D002713:Chlorine / Q000032:analysis / Q000494:pharmacology; D015169:Colony Count, Microbial; D003257:Consumer Product Safety; D004202:Disinfectants / Q000032:analysis / Q000494:pharmacology; D004203:Disinfection / Q000379:methods*; D004305:Dose-Response Relationship, Drug; D004350:Drug Residues / Q000032:analysis; D004572:Electrolysis; D019453:Escherichia coli O157 / Q000187:drug effects / Q000254:growth & development*; D005506:Food Contamination / Q000517:prevention & control; D005516:Food Microbiology; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008089:Listeria monocytogenes / Q000187:drug effects / Q000254:growth & development*; D018551:Solanum lycopersicum / Q000382:microbiology*; D019697:Onions / Q000382:microbiology*; D012486:Salmonella typhimurium / Q000187:drug effects / Q000254:growth & development*; D012710:Serum Albumin, Bovine / Q000378:metabolism; D013997:Time Factors; D014867:Water
D004202:Disinfectants; D014867:Water; D012710:Serum Albumin, Bovine; D002713:Chlorine