Genetic analysis of nystatin and amphotericin biosynthesis.
The polyene macrolides nystatin A1 and amphotericin B are effective but toxic antifungal antibiotics that are also active against enveloped viruses, protozoan parasites and pathogenic prion proteins. This chapter describes methods for genetic manipulation of the amphotericin and nystatin producers, Streptomyces nodosus and Streptomyces noursei. These techniques have been used to engineer the biosynthesis of several analogues of both polyenes. Methods for production, identification, purification and characterization of new analogues are also discussed.
Department of Biotechnology, Norwegian University of Science and Technology, Trondheim, Norway.
Sergey
S
Zotchev
Patrick
P
Caffrey
D000666:Amphotericin B / Q000096:biosynthesis*; D002853:Chromatography, Liquid; D013058:Mass Spectrometry; D015394:Molecular Structure; D009761:Nystatin / Q000096:biosynthesis*; D013302:Streptomyces / Q000235:genetics* / Q000378:metabolism
D009761:Nystatin; D000666:Amphotericin B