Comprehensive identification of cytochrome p450 isoforms from solubility-based fractionated rat liver microsomes.
Cytochrome P450 isoforms from male rat liver microsomes were comprehensively identified using nano liquid chromatography tandem mass spectrometry (nanoLC-MS/MS). The enrichment of P450, an endomembrane-anchored heme protein, was achieved by solubility-based protein fractionation, and greatly improved the total number of identified P450 isoforms. LC-MS/MS analysis of fractions resulted in the identification of total 36 P450 isoforms. The combination of proteomic analysis and the solubility-based fractionation would provide powerful tool for the expression analysis of the superfamily proteins having great similarities between the amino acids sequences.
Yamanaka
Hidenori
H
Chemicals Assessment Center, Chemicals Evaluation and Research Institute, 1600, Shimo-Takano, Sugito-machi, Kitakatsushika-gun, Saitama 345-0043, Japan. yamanaka-hidenori@ceri.jp
Takeyoshi
Masahiro
M
Minobe
Yasushi
Y
Yakabe
Yoshikuni
Y
Takatsuki
Mineo
M
Sato
Hisaya
H
D000818:Animals; D005591:Chemical Fractionation; D002853:Chromatography, Liquid / Q000379:methods*; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D007527:Isoenzymes / Q000378:metabolism; D008297:Male; D008862:Microsomes, Liver / Q000201:enzymology*; D036103:Nanotechnology / Q000379:methods; D051381:Rats; D017207:Rats, Sprague-Dawley; D012995:Solubility; D053719:Tandem Mass Spectrometry / Q000379:methods
D007527:Isoenzymes; D003577:Cytochrome P-450 Enzyme System