Whole and strip nitrocellulose membrane as well as a new-line-immunoblotting of antigen using the chemiluminescence technique.
There are a number of techniques in the scientific world that researchers use to detect specific antigens. One such technique that has provided many advantages over typical immunochemical staining is chemiluminescence. The emission of visible radiation by compounds once exposed to sunlight has been known for centuries and currently is the main principle for chemiluminescence. Here, we introduce three different chemiluminescence techniques that are widely used in immunodetection of antigens: (a) whole membrane chemiluminescence detection, (b) strip membrane chemiluminescence detection, and (c) new line blotting chemiluminescence.
Dorri
Yaser
Y
Oklahoma Medical Research Foundation, 825 NE 13th St, Arthritis and Immunology Department, MS #24, Oklahoma City, OK, 73104, USA. Yaser.dorri@gmail.com
Khalili
Ali W
AW
Scofield
R Hal
RH
D000941:Antigens / Q000032:analysis*; D003101:Collodion / Q000737:chemistry*; D006801:Humans; D015151:Immunoblotting / Q000295:instrumentation / Q000379:methods; D008163:Luminescent Measurements / Q000295:instrumentation / Q000379:methods*; D012261:Ribonucleoproteins / Q000032:analysis; D000099207:SS-A Antigen
D000941:Antigens; D003101:Collodion; D012261:Ribonucleoproteins; D000099207:SS-A Antigen