Epitope mapping using phage display peptide libraries.
Phage libraries displaying millions of peptides with randomized sequences are extremely useful tools for mapping antibody epitopes. In many cases, antibodies are able to select peptides with reasonable affinity for their combining sites (paratopes) from these libraries. Ideally, consensus motives can be deduced from multiple peptide sequences and matched to areas of the antigen against which the antibody was raised. That way, critical components of the antibody epitope can be defined. This chapter focuses on technical details of epitope mapping employing pre-made filamentous phage peptide display libraries. Examples are given for illustration.
Böttger
Volker
V
Wilex AG, Grillparzerstr. 10, 81675 Munich, Germany.
Böttger
Angelika
A
D000595:Amino Acid Sequence; D000915:Antibody Affinity; D001666:Binding Sites, Antibody; D004797:Enzyme-Linked Immunosorbent Assay; D018604:Epitope Mapping / Q000379:methods*; D006801:Humans; D008969:Molecular Sequence Data; D019151:Peptide Library
D019151:Peptide Library