Fluorescence probe sensitive to detect G-rich target strands through quenching.
Modified fluorescent probe U(F)AA AAT CTC CGC CGC has been synthesized using the nucleoside analogue 3'-O-(N,N'-diisopropylamino-2-cyanoethoxyphosphinyl)-5'-O-(4,4'-dimethoxytrityl)-2'-O-(dansyl-1-sulfonamidohexylaminocarbonyl)uridine for hybridization studies with perfectly matched (U/A) complementary and with DNA strand having similar G-rich telomeric units at the 3'-end. The thermal stability data and decrease in fluorescence intensity due to the presence of dG units clearly demonstrated the potential application of this approach in DNA diagnostics in homogeneous hybridization assays.
Nucleic Acids Research Laboratory, Department of Chemistry, Faculty of Science, Banaras Hindu University, 221005 Varanasi, India. arvindmisra2003@yahoo.com
Arvind
A
Misra
Pratibha
P
Dwivedi
Mohammad
M
Shahid
D015342:DNA Probes / Q000737:chemistry*; D003849:Deoxyguanosine / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry*; D009693:Nucleic Acid Hybridization
D015342:DNA Probes; D005456:Fluorescent Dyes; D003849:Deoxyguanosine