Effect of intranasal administration of basic fibroblast growth factor on olfactory epithelium.
To further study the effects of basic fibroblast growth factor (bFGF) on the olfactory epithelium, bFGF was intranasally administered twice a day for 6 weeks to 2.5-month-old and 7-month-old mice. The effects were immunohistochemically examined by using antibodies against proliferating cell nuclear antigen, olfactory marker protein, and GAP43. The number of cells positive for proliferating cell nuclear antigen in the supporting cell layer increased dramatically, and that of GAP43-positive cells, or globose basal cells, increased significantly, especially in aging mice. However, no significant changes were observed in the number of olfactory marker protein-positive cells or mature olfactory receptor neurons. These results suggest that topical application of bFGF promotes proliferation of globose basal cells and supporting cells.
Department of Otolaryngology-Head and Neck Surgery, Kobe University Graduate School of Medicine, Kobe, Japan.
Tasuku
T
Nishikawa
Kiyoshi
K
Doi
Naoki
N
Ochi
Sayaka
S
Katsunuma
Ken-ichi
K
Nibu
D000281:Administration, Intranasal; D000818:Animals; D015415:Biomarkers / Q000032:analysis / Q000378:metabolism; D002454:Cell Differentiation / Q000187:drug effects* / Q000502:physiology; D049109:Cell Proliferation / Q000187:drug effects*; D004847:Epithelial Cells / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D016222:Fibroblast Growth Factor 2 / Q000378:metabolism / Q000494:pharmacology*; D019922:GAP-43 Protein / Q000032:analysis / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D055495:Neurogenesis / Q000187:drug effects / Q000502:physiology; D009457:Neuroglia / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D051842:Olfactory Marker Protein / Q000032:analysis / Q000378:metabolism; D009831:Olfactory Mucosa / Q000187:drug effects* / Q000254:growth & development* / Q000378:metabolism; D018809:Proliferating Cell Nuclear Antigen / Q000032:analysis / Q000378:metabolism
D015415:Biomarkers; D019922:GAP-43 Protein; D051842:Olfactory Marker Protein; C497598:Omp protein, mouse; D018809:Proliferating Cell Nuclear Antigen; D016222:Fibroblast Growth Factor 2