Downregulation of PLK-1 expression in kaempferol-induced apoptosis of MCF-7 cells.
The molecular mechanisms underlying the kaempferol-induced cell death have not yet been fully explained. To investigate the role of kaempferol, widely distributed in foods, in tumor progression, human breast cancer cell line, MCF-7, was treated with kaempferol. Apoptosis was indicated by the accumulation of a sub-G1 population, as well as the appearance of 4'-6-diamidino-2-phenylindole (DAPI)-stained apoptotic nuclei in the MCF-7 cells after the administration of kaempferol. Western blot analysis showed cleavage of Poly (ADP-ribose) polymerase (PARP), caspase-7, Bax, and caspase-9 indicating that the intracellular pathway of apoptosis was involved. Kaempferol also downregulated the expression of polo-like kinase 1 (PLK-1), which has been reported to regulate mitotic progression and to be upregulated in several human tumors. Taken together, these findings indicate that kaempferol-induced apoptosis by initiation of intrinsic caspase cascade and downregulation of PLK-1 expression.
Kang
Gum-Yong
GY
Institute of Biomedical Science and Technology, Department of Molecular Biotechnology, Konkuk University, Seoul, Republic of Korea.
Lee
Eung-Ryoung
ER
Kim
Jung-Hyun
JH
Jung
Jin Woo
JW
Lim
Jun
J
Kim
Sang Kyung
SK
Cho
Ssang-Goo
SG
Kim
Kwang Pyo
KP
D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D053453:Caspase 9 / Q000378:metabolism; D018797:Cell Cycle Proteins / Q000235:genetics*; D045744:Cell Line, Tumor; D015536:Down-Regulation / Q000187:drug effects*; D004789:Enzyme Activation / Q000187:drug effects; D006801:Humans; D042541:Intracellular Space / Q000187:drug effects / Q000378:metabolism; D044949:Kaempferols / Q000494:pharmacology*; D017346:Protein Serine-Threonine Kinases / Q000235:genetics*; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D015398:Signal Transduction / Q000187:drug effects; D000097869:Polo-Like Kinase 1
D053453:Caspase 9; D018797:Cell Cycle Proteins; D044949:Kaempferols; D017346:Protein Serine-Threonine Kinases; D011518:Proto-Oncogene Proteins; D000097869:Polo-Like Kinase 1; C006552:kaempferol