The effect of particle size on the bioavailability of estrogenic chemicals from sediments.
The effects of particle size on the bioavailability of estrogenic chemicals in the sediments from the Yeongsan River and its tributaries in South Korea were evaluated for 2006 and 2007. Samples for chemical analysis and bioassays were collected from 6 sampling sites during both dry and rainy seasons. The pore water of the sediment samples was extracted, and estrogenic chemicals were eluted using a liquid-liquid extraction (LLE) method. Concentrations of 4-nonylphenol (NP), 4-tert-octylphenol (OP), bisphenol A (BPA), estrone (E1), 17beta-estradiol (E2), 17 alpha-ethynylestradiol (EE2) and genistein (Gen) were analyzed by gas chromatography-mass spectrometry (GC-MS). To evaluate bioavailability, hepatic vitellogenin (Vtg) concentrations of male Japanese medaka were measured after exposure to the sediment or its fractions for 7d. NP, BPA and E2 were detected in all the sediment sample extracts from the Yeongsan River and its tributaries. The concentrations of NP in the sedimentary samples ranged from 60 to 400 ngg(-1) on a dry weight basis. Similarly, OP and E2 were detected in nearly all the sediment extracts, with concentrations of 13 and 26 ngg(-1), respectively. According to the bioassay test results, all the sediment samples significantly induced Vtg in male fish after 7d of exposure. Fractionation of sediments into different size-classes (i.e., particle size >1 microm, particle size <1 microm) eliminated bioavailable estrogenic activity, but fine particles of less than 1microm in size increased the absorption of E2 from E2-amended sediment particle fractions. Consequently, the study suggested that the presence of particles and its interaction in the water environment might change the bioavailability of estrogenic chemicals.
Department of Environmental Science and Engineering, Gwangju Institute of Science and Technology, 1-Oryongdong, Bukgu, Gwangju 500-712, South Korea.
Cuong Ngoc
CN
Duong
Daniel
D
Schlenk
Nam Ik
NI
Chang
Sang Don
SD
Kim
D000818:Animals; D001682:Biological Availability; D004784:Environmental Monitoring; D004967:Estrogens / Q000032:analysis / Q000737:chemistry*; D019015:Geologic Sediments / Q000737:chemistry*; D008297:Male; D009990:Oryzias / Q000378:metabolism; D010316:Particle Size; D045483:Rivers; D012989:Soil Pollutants / Q000032:analysis / Q000737:chemistry*; D014819:Vitellogenins / Q000032:analysis
D004967:Estrogens; D012989:Soil Pollutants; D014819:Vitellogenins