Flavin mononucleotide-based fluorescent protein as an oxygen-independent reporter in Candida albicans and Saccharomyces cerevisiae.
Hypoxia is encountered frequently by pathogenic and apathogenic fungi. A codon-adapted gene encoding flavin mononucleotide-based fluorescent protein (CaFbFP) was expressed in Candida albicans and Saccharomyces cerevisiae. Both species produced CaFbFP and fluoresced even during hypoxia, suggesting that oxygen-independent CaFbFP is a useful, novel tool for monitoring hypoxic gene expression in fungi.
Institut für Mikrobiologie, Forschungszentrum Jülich, Heinrich-Heine-Universität Düsseldorf, Düsseldorf, Germany.
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D
Tielker
I
I
Eichhof
K-E
KE
Jaeger
J F
JF
Ernst
D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D002176:Candida albicans / Q000235:genetics / Q000378:metabolism*; D005486:Flavin Mononucleotide / Q000378:metabolism*; D015870:Gene Expression; D017930:Genes, Reporter; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D010100:Oxygen / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*
D001426:Bacterial Proteins; D008164:Luminescent Proteins; D005486:Flavin Mononucleotide; D010100:Oxygen