Western blotting : remembrance of past things.
Western blotting sprung from the need to develop a sensitive visual assay for the antigen specificity of monoclonal antibodies. The technique employed SDS-PAGE of protein antigens, electrophoretic replica transfer of gel-resolved proteins to unmodified nitrocellulose sheets, probing the immobilized antigens with hybridomas, and detection of antibody-antigen complexes with radiolabeled staphylococcal protein A and autoradiography. The simplicity and relevance of the method has led to its expansive application as an immunodiagnostic and a ubiquitous research tool in biology and medicine.
Burnette
W Neal
WN
Molecular Pharmaceutics Corporation, 85423 Dudley Drive, Chapel Hill, NC, 27517, USA. burnetten@iname.com
D000911:Antibodies, Monoclonal / Q000276:immunology; D000918:Antibody Specificity / Q000276:immunology; D015153:Blotting, Western / Q000266:history / Q000379:methods; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D049673:History, 20th Century; D017712:Peer Review, Research; D010506:Periodicals as Topic
D000911:Antibodies, Monoclonal