Nucleic acid aptamer molecular recognition principles and application in liquid chromatography and capillary electrophoresis.
Nucleic acid aptamers isolated from the systematic evolution of ligands by exponential enrichment (SELEX) have the capacity to recognize various classes of target molecules with high affinity and specificity. In this context, the development of aptamer-based molecular recognition tools has become a very interesting and promising analytical strategy during the last few years. In this review, the molecular recognition features of aptamers as well as the key factors for their practical applicability to the chromatographic and capillary electrophoretic fields are summarized.
Peyrin
Eric
E
Département de Pharmacochimie Moléculaire UMR 5063 CNRS, Institut de Chimie Moléculaire de Grenoble FR 2607, Université Grenoble I (Joseph Fourier), Grenoble, France. eric.peyrin@ujf-grenoble.fr
D052157:Aptamers, Nucleotide / Q000737:chemistry*; D002853:Chromatography, Liquid / Q000379:methods*; D019075:Electrophoresis, Capillary / Q000379:methods*; D007700:Kinetics; D012680:Sensitivity and Specificity
D052157:Aptamers, Nucleotide