[The regularities of the induction of DNA double strand breaks and DNA repair in human lymphocytes after irradiation by accelerated heavy ions with different energy].
The regularities of the induction of DNA double strand breaks (DSB) in human lymphocytes after irradiation by different doses of accelerated lithium and carbon ions (33 and 480 MeV/nucleon, LET = 20 and 10.6 keV/microm, respectively) and gamma-rays 60Co by using of comet assay were investigated. It was shown that the dependence of DSB formation increases linearly with growing of the dose of lithium and carbon ions and gamma-rays. The biological effectiveness of carbon ions with high energy was similar with gamma-rays, lithium ions possess greater biological effectiveness in comparison with gamma-rays and value of RBE of lithium ions amount 1.6 +/- 0.1. The kinetic of DNA repair from DSB in human lymphocytes after irradiation by lithium and carbon ions and gamma-rays was studied. It is revealed that the reparation proceeds effectively with heavy ion and gamma-ray irradiation by exponential kinetics.
V N
VN
Chausov
A V
AV
Boreĭko
E A
EA
Krasavin
A V
AV
Mozhaeva
I I
II
Ravnachka
S I
SI
Tiunchik
V A
VA
Tronov
D002250:Carbon Radioisotopes / Q000009:adverse effects; D020552:Comet Assay; D053903:DNA Breaks, Double-Stranded; D004260:DNA Repair; D004307:Dose-Response Relationship, Radiation; D005720:Gamma Rays / Q000009:adverse effects*; D020450:Heavy Ions / Q000009:adverse effects*; D006801:Humans; D008094:Lithium / Q000009:adverse effects; D008214:Lymphocytes / Q000528:radiation effects*
D002250:Carbon Radioisotopes; D008094:Lithium