Comprehensive proteome analysis of lettuce latex using multidimensional protein-identification technology.
Commercially, lettuce (Lactuca sativa) is one of the most important leafy vegetables. Lettuce produces a milky latex of variable chemical compositions within its laticifers. As a step toward understanding the main physiological roles of this latex in higher plants, we embarked on its proteomic analysis. We investigated 587 latex proteins that were identified from the lettuce latex using multidimensional protein-identification technology. A bioinformatics analysis showed that the most frequently encountered proteins in the latex were organellar proteins from plastids and mitochondria, followed by nucleic and cytoplasmic proteins. Functional classification of the identified proteins showed that proteins related to metabolism, cell rescue, defense, and virulence were the most abundant in lettuce latex. Furthermore, numerous resistance proteins of lettuce and viral proteins were present in the latex suggesting for the first time a possible function of the lettuce latex in defense or pathogenesis. To the knowledge of the authors, this is the first large-scale proteome analysis of lettuce latex.
Division of Applied Life Science (BK21 program), Environmental Biotechnology, National Core Research Center, PMBBRC, Gyeongsang National University, Jinju 660-701, Republic of Korea.
Won Kyong
WK
Cho
Xiong-Yan
XY
Chen
Nazim Mohamad
NM
Uddin
Yeonggil
Y
Rim
Juyeon
J
Moon
Jin-Hee
JH
Jung
Chunlin
C
Shi
Hyosub
H
Chu
Suwha
S
Kim
Seon-Won
SW
Kim
Zee-Yong
ZY
Park
Jae-Yean
JY
Kim
D002853:Chromatography, Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D007840:Latex / Q000737:chemistry*; D018545:Lactuca / Q000737:chemistry*; D010940:Plant Proteins / Q000737:chemistry* / Q000502:physiology; D020543:Proteome; D013347:Subcellular Fractions / Q000378:metabolism; D053719:Tandem Mass Spectrometry
D007840:Latex; D010940:Plant Proteins; D020543:Proteome