[Optimization on the production of analgesic peptide from Buthus martensii Karsch in Pichia pastoris].
The technology of liquid fermentation for producing the recombinant analgesic peptide BmK AngM1 from Buthus martensii Karsch in Pichia pastoris was studied by single-factor and orthogonal test. The results showed that the optimal culture conditions were as follows: 1.2% methanol, 0.6% casamino acids, initial pH 6.0, and three times of basal inoculation volume. Under the above culture conditions, the expression level of recombinant BmK AngM1 in Pichia pastoris was above 500 mg x L(-1), which was more than three times of the control. The study has laid a foundation for the large-scale preparation of BmK AngM1 to meet the needs of theoretical research of BmK AngM1 and development of new medicines.
Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Key Laboratory of Biosynthesis of Natural Products, Ministry of Health of PRC, Beijing 100050, China.
Jin-ling
JL
Yang
Hui-xia
HX
He
Hui-xin
HX
Zhu
Ke-di
KD
Cheng
Ping
P
Zhu
D000596:Amino Acids / Q000494:pharmacology; D000700:Analgesics / Q000302:isolation & purification / Q000378:metabolism; D000818:Animals; D005285:Fermentation; D015870:Gene Expression; D006863:Hydrogen-Ion Concentration; D000432:Methanol / Q000494:pharmacology; D010455:Peptides / Q000302:isolation & purification / Q000378:metabolism*; D010843:Pichia / Q000235:genetics / Q000378:metabolism; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D012604:Scorpion Venoms / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D012605:Scorpions / Q000737:chemistry*
D000596:Amino Acids; D000700:Analgesics; D010455:Peptides; D011994:Recombinant Proteins; D012604:Scorpion Venoms; C017721:casamino acids; D000432:Methanol