Involvement of branched units at position 6 in the reactivity of a unique variety of beta-D-glucan from Aureobasidium pullulans to antibodies in human sera.
We recently determined the structure of a unique type of 1,3-beta-D-glucan obtained from Aureobasidium pullulans (AP-FBG) and found that it reacted with the antibodies in human sera. The reactivity of AP-FBG to the antibodies was stronger than that of 1,3-beta-D-glucan obtained Grifola frondosa (GRN) but weaker than that of 1,3-beta-D-glucan from Candida albicans (CSBG). Here, we demonstrated that AP-FBG reacted to IgG antibodies, especially those of the subclasses IgG2, IgG1, and IgG3, in human sera. Moreover, the results of competitive enzyme-linked immunosorbent assays (ELISAs) using various glucan competitors showed that these IgGs recognized branched chains at position 6. This is the first study to report that the branched chains at position 6 of beta-D-glucan strongly contribute to its recognition by antibodies in human sera. This high reactivity of AP-FBG to human IgG could be advantageous for the use of this glucan in medicine, e.g., as an immunostimulatory agent.
Laboratory for Immunopharmacology of Microbial Products, School of Pharmacy, Tokyo University of Pharmacy and Life Sciences, Tokyo 192-0392, Japan.
Rui
R
Tada
Asuka
A
Tanioka
Ken-ichi
K
Ishibashi
Yoshiyuki
Y
Adachi
Kazufumi
K
Tsubaki
Naohito
N
Ohno
D001203:Ascomycota / Q000737:chemistry*; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood* / Q000276:immunology*; D047071:beta-Glucans / Q000737:chemistry* / Q000276:immunology*; D005719:gamma-Globulins / Q000276:immunology
D007074:Immunoglobulin G; D047071:beta-Glucans; D005719:gamma-Globulins