Cationic electrophoresis and eastern blotting.
Denaturing, discontinuous electrophoresis in the presence of SDS has become a standard method for the protein scientist. However, there are situations where this method produces suboptimal results. In these cases, electrophoresis in the presence of positively charged detergents like cetyltrimethylammonium bromide may work considerably better. Methods for electrophoresis, staining, and blotting of such gels are presented.
Department of Biochemistry, Ross University School of Medicine, Box 266, Portsmouth Campus, Roseau, Commonwealth of Dominica, West Indies. engelbert_buxbaum@web.de
Engelbert
E
Buxbaum
D002412:Cations / Q000737:chemistry*; D000077286:Cetrimonium; D002593:Cetrimonium Compounds / Q000737:chemistry; D003902:Detergents / Q000737:chemistry; D004591:Electrophoresis, Polyacrylamide Gel / Q000295:instrumentation / Q000379:methods*; D015151:Immunoblotting / Q000295:instrumentation / Q000379:methods*; D011506:Proteins / Q000032:analysis*
D002412:Cations; D002593:Cetrimonium Compounds; D003902:Detergents; D011506:Proteins; D000077286:Cetrimonium