Evaluation of potential for inhibition of growth of Escherichia coli O157:H7 and multidrug-resistant Salmonella serovars in raw beef by addition of a presumptive Lactobacillus sakei ground beef isolate.
The efficacy of adding presumptive Lactobacillus sakei (LS) strain 10-EGR-a, the most inhibitory from among 12 ground beef Lactobacillus isolates, to inhibit growth by Escherichia coli O157:H7 and multidrug-resistant (MDR) Salmonella (serovars Newport and Typhimurium) was evaluated in a beef-derived broth medium at 10 degrees C and in fresh raw ground beef at 10 and 5 degrees C. Pathogen inhibition was observed in the broth medium at both high (10(8):10(5) to 10(7):10(5)) and low (10(6):10(5) to 10(5):10(5)) LS:pathogen ratios. After 9 days at 10 degrees C, in broth medium with high LS:pathogen ratios, growth of E. coli O157:H7 and MDR Salmonella was inhibited by an average of 2.6 and 3.2 log CFU/ml, respectively, whereas in broth medium with low LS:pathogen ratios, E. coli O157:H7 and MDR Salmonella growth was inhibited by an average of 2.8 and 1.8 log CFU/ml, respectively. However, in raw ground beef no significant inhibition was seen with LS:pathogen ratios of 10(5):10(2) to 10(5):10(3). Significant inhibition was seen at very high LS:pathogen ratios (10(6) to 10(7):10(2) to 10(3)), but gross spoilage of the product occurred by day 6. Although presumptive LS 10-EGR-a can inhibit growth of E. coli O157:H7 and MDR Salmonella in a beef-derived broth medium, the inability to produce similar results in ground beef without deleteriously affecting the quality of the product is a limitation that needs further investigation.
Department of Food Science, University of Wisconsin, Madison, Wisconsin 53706, USA.
John R
JR
Ruby
Steven C
SC
Ingham
D000818:Animals; D000898:Antibiosis; D002417:Cattle; D015169:Colony Count, Microbial; D024901:Drug Resistance, Multiple, Bacterial; D019453:Escherichia coli O157 / Q000254:growth & development*; D005511:Food Handling / Q000379:methods; D005516:Food Microbiology; D005519:Food Preservation / Q000379:methods*; D006801:Humans; D007778:Lactobacillus / Q000502:physiology*; D008461:Meat Products / Q000382:microbiology*; D012475:Salmonella / Q000187:drug effects / Q000254:growth & development*; D013696:Temperature; D013997:Time Factors