Nanometer distance measurements between multicolor quantum dots.
Quantum dot dimers made of short double-stranded DNA molecules labeled with different color quantum dots at each end were imaged using multicolor stage-scanning confocal microscopy. This approach eliminates chromatic aberration and color registration issues usually encountered in other multicolor imaging techniques. We demonstrate nanometer accuracy in individual distance measurement by suppression of quantum dot blinking and thoroughly characterize the contribution of different effects to the variability observed between measurements. Our analysis opens the way to accurate structural studies of biomolecules and biomolecular complexes using multicolor quantum labeling.
Antelman
Josh
J
Department of Chemistry & Biochemistry, University of California at Los Angeles, Los Angles, California 90095, USA.
Wilking-Chang
Connie
C
Weiss
Shimon
S
Michalet
Xavier
X
D004247:DNA / Q000737:chemistry*; D019281:Dimerization; D004548:Elasticity; D018613:Microscopy, Confocal; D046529:Microscopy, Electron, Transmission; D045663:Quantum Dots
D004247:DNA