Change of serum vascular adhesion protein-1 after glucose loading correlates to carotid intima-medial thickness in non-diabetic subjects.
BACKGROUND
We investigated if serum vascular adhesion protein-1 (SSAO/VAP-1) changed acutely following oral glucose loading and whether such changes are correlated with surrogate markers of atherosclerosis.


METHODS
A total of 115 non-diabetics subjects were enrolled for an oral glucose tolerance test (OGTT). Carotid intima-medial thickness (IMT) was measured by ultrasonography. Serum SSAO/VAP-1 was analyzed by time-resolved immunofluorometric assay. Serum thiobarbituric acid reactive substances (TBARS) and advanced glycated end products (AGEs) were measured by fluorometric assays.


RESULTS
Serum SSAO/VAP-1 increased significantly at 30 min after oral glucose loading and lasted to 2 h (p=0.0005 and p<0.0001, for 30 min and 2 h respectively). The area under curve of serum SSAO/VAP-1 during OGTT (AUC-VAP-1) correlated significantly with carotid IMT, independent of age, gender, low-density lipoprotein cholesterol, systolic blood pressure, hemoglobin A1c, serum TBARS, AGEs, and high-sensitivity C-reactive protein. Subjects with a positive AUC-VAP-1 had significantly higher serum TBARS and AGEs than subjects with a negative AUC-VAP-1 adjusted for age and gender.


CONCLUSIONS
Serum SSAO/VAP-1 changed acutely following oral glucose loading in non-diabetic subjects. Change of serum SSAO/VAP-1 correlated independently to serum TBARS, AGEs, and carotid IMT. Our findings suggest that acute change of serum SSAO/VAP-1 is a novel marker for hyperglycemia-induced atherosclerosis.
Department of Internal Medicine, National Taiwan University Hospital, Taipei, Taiwan.
Hung-Yuan
HY
Li
Mao-Shin
MS
Lin
Jung-Nan
JN
Wei
Chi Sheng
CS
Hung
Fu-Tien
FT
Chiang
Cheng-Hsin
CH
Lin
Hsiu-Ching
HC
Hsu
Chien-Yin
CY
Su
Mei-Yu
MY
Wu
David J
DJ
Smith
Jani
J
Vainio
Ming-Fong
MF
Chen
Lee-Ming
LM
Chuang
D006631:Amine Oxidase (Copper-Containing) / Q000097:blood* / Q000378:metabolism; D050197:Atherosclerosis / Q000097:blood / Q000473:pathology; D002339:Carotid Arteries / Q000473:pathology*; D015815:Cell Adhesion Molecules / Q000097:blood* / Q000378:metabolism; D003920:Diabetes Mellitus; D005215:Fasting; D005260:Female; D005947:Glucose / Q000494:pharmacology*; D017127:Glycation End Products, Advanced; D006801:Humans; D008297:Male; D008875:Middle Aged; D017539:Tunica Intima / Q000473:pathology*
D015815:Cell Adhesion Molecules; D017127:Glycation End Products, Advanced; C076668:AOC3 protein, human; D006631:Amine Oxidase (Copper-Containing); D005947:Glucose