Engineered SERS substrates with multiscale signal enhancement: nanoparticle cluster arrays.
Defined nanoparticle cluster arrays (NCAs) with total lateral dimensions of up to 25.4 microm x 25.4 microm have been fabricated on top of a 10 nm thin gold film using template-guided self-assembly. This approach provides precise control of the structural parameters in the arrays, allowing a systematic variation of the average number of nanoparticles in the clusters (n) and the edge-to-edge separation (Lambda) between 1 < n < 20 and 50 nm < or = Lambda < or = 1000 nm, respectively. Investigations of the Rayleigh scattering spectra and surface-enhanced Raman scattering (SERS) signal intensities as a function of n and Lambda reveal direct near-field coupling between the particles within individual clusters, whose strength increases with the cluster size (n) until it saturates at around n = 4. Our analysis shows that strong near-field interactions between individual clusters significantly affect the SERS signal enhancement for edge-to-edge separations Lambda < 200 nm. The observed dependencies of the Raman signals on n and Lambda indicate that NCAs support a multiscale signal enhancement which originates from simultaneous inter- and intracluster coupling and |E|-field enhancement. The NCAs provide strong and reproducible SERS signals not only from small molecules but also from whole bacterial cells, which enabled a rapid spectral discrimination between three tested bacteria species: Escherichia coli, Bacillus cereus, and Staphylococcus aureus.
Department of Chemistry, Boston University, Boston, Massachusetts 02215, USA.
Bo
B
Yan
Anupama
A
Thubagere
W Ranjith
WR
Premasiri
Lawrence D
LD
Ziegler
Luca
L
Dal Negro
Björn M
BM
Reinhard
D003460:Crystallization / Q000379:methods*; D006046:Gold / Q000737:chemistry*; D046911:Macromolecular Substances / Q000737:chemistry; D008968:Molecular Conformation; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure*; D036103:Nanotechnology / Q000379:methods*; D010316:Particle Size; D013059:Spectrum Analysis, Raman / Q000379:methods*; D013499:Surface Properties
D046911:Macromolecular Substances; D006046:Gold