FATP1 localizes to mitochondria and enhances pyruvate dehydrogenase activity in skeletal myotubes.
Fatty acid transport protein 1 (FATP1) has been previously immunolocalized in intracellular compartments. Here we show that FATP1 localizes to the mitochondria in cultured myotubes, by immunoblots of subcellular fractions and immunocytology of the fusion protein FATP1-GFP. FATP1 strongly stimulates CO(2) production from glucose whereas nonmitochondrial metabolism of glucose is only slightly enhanced. FATP1 raises the activity and activates the pyruvate dehydrogenase (PDH) complex and the pyruvate decarboxylase PDH-E1 catalytic subunit, without changing E2, E3BP or E1alpha and increasing E1beta protein content. These data reveals the localization and points to a regulatory function of FATP1 in myotube mitochondria.
Guitart
Maria
M
Departament de Bioquímica i Biologia Molecular, Facultat de Biologia, Universitat de Barcelona, CIBER de Diabetes y Enfermedades Metabólicas Asociadas, IBUB, Diagonal, 645, E-08028 Barcelona, Spain. maguita022002@yahoo.es
Andreu
Antonio L
AL
García-Arumi
Elena
E
Briones
Paz
P
Quintana
Ester
E
Gómez-Foix
Anna M
AM
García-Martínez
Celia
C
D002458:Cell Fractionation; D002478:Cells, Cultured; D050612:Fatty Acid Transport Proteins / Q000032:analysis*; D017930:Genes, Reporter; D049452:Green Fluorescent Proteins / Q000032:analysis; D006801:Humans; D015151:Immunoblotting; D007658:Ketone Oxidoreductases / Q000378:metabolism*; D008856:Microscopy, Fluorescence; D008928:Mitochondria / Q000737:chemistry* / Q000378:metabolism; D018485:Muscle Fibers, Skeletal / Q000737:chemistry* / Q000378:metabolism; D018482:Muscle, Skeletal / Q000737:chemistry* / Q000378:metabolism
D050612:Fatty Acid Transport Proteins; C493568:SLC27A1 protein, human; D049452:Green Fluorescent Proteins; D007658:Ketone Oxidoreductases; C053164:pyruvate dehydrogenase (NADP+)