Mass spectrometry assisted lithography for the patterning of cell adhesion ligands on self-assembled monolayers.
Pattern of events: A simple and flexible method has been developed for patterning cell adhesion ligands. Locally erasing self-assembled monolayers with tri(ethyleneglycol) groups on a gold substrate by using a MALDI-TOF MS nitrogen laser and filling the exposed gold surface with an alkanethiol presenting carboxylic acid groups enables subsequent immobilization of maleimide and a cell adhesion peptide, which can then recognize cells (see scheme).
Department of Chemistry and School of Molecular Science (BK21), Institute for the BioCentury, Korea Advanced Institute of Science and Technology, 373-1 Guseong-dong, Yuseong-gu, Daejeon 305-701, Korea.
Young-Kwan
YK
Kim
Soo-Ryoon
SR
Ryoo
Sul-Jin
SJ
Kwack
Dal-Hee
DH
Min
D002448:Cell Adhesion; D015815:Cell Adhesion Molecules / Q000737:chemistry*; D005026:Ethylene Glycols / Q000737:chemistry; D006046:Gold / Q000737:chemistry; D055767:Immobilized Proteins / Q000737:chemistry*; D008024:Ligands; D008301:Maleimides / Q000737:chemistry; D009842:Oligopeptides / Q000737:chemistry*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D013499:Surface Properties
D015815:Cell Adhesion Molecules; D005026:Ethylene Glycols; D055767:Immobilized Proteins; D008024:Ligands; D008301:Maleimides; D009842:Oligopeptides; C043592:maleimide; D006046:Gold