TLC blot (far-eastern blot) and its applications.
A simple method for transfer of lipids including phospholipids, glycolipids, and neutral lipids from a high-performance thin-layer chromatography (HPTLC) plate to a polyvinylidene difluoride (PVDF) membrane, called TLC blot (far-eastern blot), is presented. Lipids separated on a HPTLC plate are blotted quantitatively. This procedure made it possible to purify individual lipids from a blotted membrane in a short time. Binding study, immunodetection, and mass spectrometric analysis are available for PVDF membrane. Furthermore, the world of molecular species imaging is opened by a scanning analysis with a combination of TLC blot and matrix-assisted laser desorption/ionization quadrupole ion trap time-of-flight mass spectrometer (TLC-Blot/MALDI-TOF MS).
Taki
Takao
T
Institute of Biomedical Innovation, Otsuka Pharmaceutical Co. Ltd., 463-10 Kagasuno, Kawauchi-cho Tokushima, 771-0192, Japan. t_taki@research.otsuka.co.jp
Gonzalez
Tania Valdes
TV
Goto-Inoue
Naoko
N
Hayasaka
Takahiro
T
Setou
Mitsutoshi
M
D000818:Animals; D001923:Brain Chemistry; D002478:Cells, Cultured; D002855:Chromatography, Thin Layer / Q000295:instrumentation / Q000379:methods*; D006017:Glycolipids / Q000032:analysis*; D006028:Glycosphingolipids / Q000032:analysis*; D008055:Lipids / Q000032:analysis*; D010743:Phospholipids / Q000032:analysis*; D011145:Polyvinyls / Q000737:chemistry; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods; D005465:Fluorocarbon Polymers
D006017:Glycolipids; D006028:Glycosphingolipids; D008055:Lipids; D010743:Phospholipids; D011145:Polyvinyls; C024865:polyvinylidene fluoride; D005465:Fluorocarbon Polymers