EQCM biosensors based on DNA aptamers and antibodies for rapid detection of prions.
Novel affinity biosensors for detecting cellular prions, PrP(C), based on DNA aptamers and antibodies immobilized onto the carbon nanotubes have been designed and compared in accordance with their binding ability and analytical performance. The biosensors allowed us to detect PrP(C) with the limits of detection of 20 to 50 pM.
Department of Nuclear Physics and Biophysics, Comenius University, Mlynska dolina F1, 842 48 Bratislava, Slovakia. tibor.hianik@fmph.uniba.sk
T
T
Hianik
A
A
Porfireva
I
I
Grman
G
G
Evtugyn
D000906:Antibodies / Q000032:analysis*; D052157:Aptamers, Nucleotide; D015374:Biosensing Techniques / Q000379:methods*; D055767:Immobilized Proteins; D018621:PrPC Proteins / Q000032:analysis* / Q000276:immunology; D011328:Prions / Q000032:analysis* / Q000276:immunology
D000906:Antibodies; D052157:Aptamers, Nucleotide; D055767:Immobilized Proteins; D018621:PrPC Proteins; D011328:Prions