In vitro effect of vitamin E on lectin-stimulated porcine peripheral blood mononuclear cells.
In order to analyze the effect of vitamin E on Th1 and Th2 cytokine production, porcine peripheral blood mononuclear cells (PBMC) were isolated from healthy pigs (n=8) and cultured with either 0, 10, 50, or 100muM of vitamin E (alpha-tocopherol). PBMC were stimulated with PHA for either, 24h to determine: (a) the concentration of tocopherol incorporated into the cell membrane, (b) cytokine production and (c) Th1 and Th2 regulators gene expression; or 72h to determine the proliferation of PBMC. Vitamin E was incorporated into the PBMC in a dose dependent manner, giving as a result a high proliferation of cells irrespective of the dose of vitamin E used. Regarding cytokine production, vitamin E consistently decreases the mRNA expression and the percentage of cells producing IL-10. Vitamin E did not influence the production of IFN-gamma but the lowest level of vitamin E (10muM) was sufficient to maximally increase the proportion of cells producing IL-2, to diminish IL-4, and discreetly increase the mRNA expression of TBX21 vs. GATA3. In conclusion, our results revealed that vitamin E is able to suppress IL-10 production and to influence the production of IL-2, IL-4, and maybe TBX21. Vitamin E clearly has immunomodulatory effects, though further work in vivo to determine the physiological nature of these effects is warranted.
Centro de Investigación en Alimentación y Desarrollo, A.C., Apdo. Postal No. 1735, Hermosillo 83000 Sonora, Mexico. jhdez@ciad.mx
Jesús
J
Hernández
Eneida
E
Soto-Canevett
Araceli
A
Pinelli-Saavedra
Mónica
M
Resendiz
Silvia Y
SY
Moya-Camarena
Kirk C
KC
Klasing
D000818:Animals; D016207:Cytokines / Q000096:biosynthesis; D050990:GATA3 Transcription Factor / Q000235:genetics; D008213:Lymphocyte Activation / Q000187:drug effects*; D010835:Phytohemagglutinins / Q000494:pharmacology; D012333:RNA, Messenger / Q000032:analysis; D013552:Swine; D020825:T-Box Domain Proteins / Q000235:genetics; D018417:Th1 Cells / Q000276:immunology; D018418:Th2 Cells / Q000276:immunology; D014810:Vitamin E / Q000493:pharmacokinetics / Q000494:pharmacology*; D000098815:T-bet Transcription Factor
D016207:Cytokines; D050990:GATA3 Transcription Factor; D010835:Phytohemagglutinins; D012333:RNA, Messenger; D020825:T-Box Domain Proteins; D000098815:T-bet Transcription Factor; D014810:Vitamin E