Down-regulation of miR-141 in gastric cancer and its involvement in cell growth.
OBJECTIVE
Human microRNA-141 (miR-141), a member of the miR-200 family, has been reported to be associated with various human malignancies. However, it remains unknown whether miR-141 is involved in the pathogenesis of gastric cancer. Therefore, we examined the expression of miR-141 in gastric cancer tissues and the effect of miR-141 overexpression on cancer cell proliferation.


METHODS
The expression level of miR-141 in 35 pair-matched gastric neoplastic and adjacent non-neoplastic tissues, and in 5 gastric cancer cell lines were examined by quantitative real-time PCR. The growth of MGC-803 cells transfected with miRNA precursor was examined by MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl-tetrazoliumbromide) assay.


RESULTS
MiR-141 was significantly down-regulated in 80% (28/35) of primary gastric cancer tissues compared with pair-matched adjacent non-tumor tissues (P < 0.01). The expression of miR-141 was also found to be substantially reduced in several human gastric cancer cell lines such as MGC-803, HGC-27, SGC-7901 and BGC-823 cells. Overexpression of miR-141 with its precursors significantly inhibited the proliferation of gastric cancer cells.


CONCLUSIONS
These results suggest that miR-141 may be involved in the development of gastric cancer through its inhibitory effect on cell proliferation.
Gastroenterology Laboratory, Clinical Research Institute, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, 310016 Hangzhou, People's Republic of China.
Ying
Y
Du
Yanjun
Y
Xu
Ling
L
Ding
Haomi
H
Yao
Hong
H
Yu
Tianhua
T
Zhou
Jianmin
J
Si
D000368:Aged; D000369:Aged, 80 and over; D049109:Cell Proliferation; D015536:Down-Regulation / Q000502:physiology; D005260:Female; D015972:Gene Expression Regulation, Neoplastic / Q000502:physiology; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000378:metabolism / Q000502:physiology*; D008875:Middle Aged; D013274:Stomach Neoplasms / Q000235:genetics / Q000473:pathology / Q000503:physiopathology*; D014407:Tumor Cells, Cultured
C533895:MIRN141 microRNA, human; D035683:MicroRNAs