Retention data from reverse-phase high-performance thin-layer chromatography in characterization of some bis-salicylic acid derivatives.
The chromatographic behaviour of salicylic acid derivatives was investigated using reversed-phase high performance thin-layer chromatography (RP HPTLC) with methanol-water and dioxane-water binary mixtures as mobile phase in order to establish relationships between chromatographic data and selected physico-chemical parameters that are related to ADME (absorption, distribution, metabolism and elimination). Some of the investigated compounds were screened for antioxidant activity. Examination of chromatographic behaviour revealed a linear correlation between R(M) values and the volume fraction of mobile phase modifier. Obtained R(M)(0) values were correlated with lipophilicity, solubility, human intestinal absorption, plasma-protein binding, and blood-brain barrier data. The comparison among chromatographic data obtained by two mobile phase was performed with a statistical technique, principle component analysis.
Department of Chemistry, Faculty of Sciences, University of Novi Sad, Novi Sad, Serbia.
Tatjana
T
Djaković-Sekulić
Nada
N
Perisić-Janjić
Evgenija
E
Djurendi
D000975:Antioxidants / Q000032:analysis* / Q000737:chemistry / Q000493:pharmacokinetics; D002855:Chromatography, Thin Layer / Q000379:methods*; D004146:Dioxanes; D006801:Humans; D007408:Intestinal Absorption; D008055:Lipids / Q000737:chemistry; D000432:Methanol; D025341:Principal Component Analysis; D020156:Salicylic Acid / Q000032:analysis* / Q000737:chemistry / Q000493:pharmacokinetics; D012995:Solubility; D014867:Water
D000975:Antioxidants; D004146:Dioxanes; D008055:Lipids; D014867:Water; C025223:1,4-dioxane; D020156:Salicylic Acid; D000432:Methanol