Velocity modulation of microtubules in electric fields.
We show that the speed of microtubules gliding over a kinesin-coated surface can be controlled over a wide range of values by the application of an electric field. The speed can be increased by up to a factor of 5 compared to the speed at zero field when assisting forces are applied and slowed down to zero velocity for opposing fields. Sideways applied fields also induce significant motion. The kinesin surface density impacts the rate of velocity change, whereas the ATP concentration does not seem to play a major role, provided that it is nonzero. A simple grab-and-release model is presented that explains the velocity change with applied electric fields.
Dujovne
Irene
I
Delft University of Technology, Kauli Institute of Nanoscience, Section Molecular Biophysics, Lorentzweg 1, 2628 CJ, The Netherlands.
van den Heuvel
Martin
M
Shen
Yi
Y
de Graaff
Martijn
M
Dekker
Cees
C
D004560:Electricity; D016547:Kinesins / Q000378:metabolism; D008870:Microtubules / Q000378:metabolism / Q000502:physiology*
D016547:Kinesins