Decoding the key aroma compounds of a Hungarian-type salami by molecular sensory science approaches.
Application of aroma extract dilution analysis on an extract/distillate prepared from a Hungarian-type salami and subsequent identification experiments led to the identification of 51 of 55 odor-active compounds detected in the flavor dilution (FD) factor range of 16-4096. Nineteen of these compounds are reported for the first time as aroma components of dry-fermented sausages, among them 3-hydroxy-4,5-dimethyl-2(5H)-furanone (sotolon), trans-4,5-epoxy-(E)-2-decenal, and bis(2-methyl-3-furyl) disulfide. The highest FD factors were found for 2-methoxyphenol (smoky, sweet), 2-methoxy-4-(2-propenyl)phenol (clove-like), 2-methoxy-4-(E)-(1-propenyl)phenol (clove-like), and acetic acid (pungent, sour). Forty-five aroma compounds were subsequently quantified using stable isotope dilution assays, and their odor activity values (OAVs; ratio of concentration to odor threshold) were calculated on the basis of odor thresholds in oil. The highest OAVs were calculated for acetic acid, acetaldehyde, 3-(methylthio)propanal, phenylacetaldehyde, 2-methoxyphenol, and 2-acetyl-1-pyrroline. A model mixture containing 33 odorants in the same concentrations as they occurred in the sausage was prepared in a matrix consisting of 44% sunflower oil and 56% aqueous phosphate buffer. A comparison of the overall aroma of this model mixture with the original showed a very high similarity, suggesting that the key aroma compounds were successfully identified.
Söllner
Kerstin
K
Lehrstuhl für Lebensmittelchemie, Technische Universitaet Muenchen, Lichtenbergstrasse 4, D-85748 Garching, Germany.
Schieberle
Peter
P
D000818:Animals; D002849:Chromatography, Gas; D003903:Deuterium; D005285:Fermentation; D006801:Humans; D006814:Hungary; D007201:Indicator Dilution Techniques; D013058:Mass Spectrometry; D008461:Meat Products / Q000032:analysis*; D009812:Odorants / Q000032:analysis*; D012903:Smell; D013649:Taste; D055549:Volatile Organic Compounds / Q000032:analysis / Q000737:chemistry
D055549:Volatile Organic Compounds; D003903:Deuterium