Fluorescence and electron microscopic localization of F-actin in the ependymocytes.
The organization of F-actin in the ventricular system has been reported to display pronounced regional differences with respect to shape, size, and development. However, the real roles played by F-actin in these cells cannot be understood unless the precise localization of F-actin is defined. In the present study, we used double-fluorescence labeling to further examine the localization of F-actin in the ependymocytes and its spatial relation to the other two cytoskeletal components, microtubules and intermediate filaments. Then we converted fluorescence signals for F-actin to peroxidase/DAB reaction products by use of a phalloidin-based FITC-anti-FITC system. This detection technique provided an overview of the distribution of F-actin in the ependymocytes at the ultrastructural level, and has been proven to be helpful in correlating light and electron microscopic investigations.
Li
Yan-Chao
YC
Neural Architecture, Advanced Technology Development Group, RIKEN Brain Science Institute, Hirosawa 2-1, Wako, Saitama 351-0198, Japan. ldlyc@brain.riken.jp
Bai
Wan-Zhu
WZ
Sakai
Kazuhisa
K
Hashikawa
Tsutomu
T
D015100:3,3'-Diaminobenzidine; D000199:Actins / Q000378:metabolism*; D000818:Animals; D002831:Choroid Plexus / Q000166:cytology / Q000378:metabolism / Q000648:ultrastructure; D004805:Ependyma / Q000166:cytology / Q000378:metabolism* / Q000648:ultrastructure; D005260:Female; D016650:Fluorescein-5-isothiocyanate; D005456:Fluorescent Dyes; D007382:Intermediate Filaments / Q000378:metabolism; D008854:Microscopy, Electron; D008856:Microscopy, Fluorescence; D008870:Microtubules / Q000378:metabolism; D010544:Peroxidases; D051381:Rats; D017207:Rats, Sprague-Dawley; D013116:Spinal Cord / Q000166:cytology / Q000378:metabolism / Q000648:ultrastructure
D000199:Actins; D005456:Fluorescent Dyes; D015100:3,3'-Diaminobenzidine; D010544:Peroxidases; D016650:Fluorescein-5-isothiocyanate