Genomic sequencing reveals regulatory mutations and recombinational events in the widely used MC4100 lineage of Escherichia coli K-12.
The genome of an Escherichia coli MC4100 strain with a lambda placMu50 fusion revealed numerous regulatory differences from MG1655, including one that arose during laboratory storage. The 194 mutational differences between MC4100(MuLac) and other K-12 sequences were mostly allocated to specific lineages, indicating the considerable mutational divergence between K-12 strains.
School of Molecular and Microbial Biosciences, University of Sydney, Sydney, New South Wales, Australia.
Thomas
T
Ferenci
Zhemin
Z
Zhou
Thu
T
Betteridge
Yan
Y
Ren
Yu
Y
Liu
Lu
L
Feng
Peter R
PR
Reeves
Lei
L
Wang
D001483:Base Sequence; D004926:Escherichia coli / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial; D016680:Genome, Bacterial; D008969:Molecular Sequence Data; D009154:Mutation; D011995:Recombination, Genetic; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA