Involvement of DNA fragmentation of enterocytes in mucosal injury to a mouse jejunum incubated in ussing chambers.
A mouse jejunum, when incubated in vitro in Ussing chambers, was found to exhibit morphological deterioration of the villi with denudation of the epithelia (J Nutr Sci Vitaminol, 51: 406, 2005). Our study examined the involvement of apoptosis in an intestinal injury model by a DNA ladder assay and terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling (TUNEL) assay. Electrophoresis of mucosal DNA revealed ladders, indicating the occurrence of DNA fragmentation. Cells with TUNEL-positive nuclei were detected among the villus epithelial cells (enterocytes), whereas they are rarely seen among crypt epithelial cells. These features were evident within 1 h after the start of incubation. Apoptotic death of the enterocytes was thus involved in the destruction of villi when incubated in Ussing chambers.
Inagaki-Tachibana
Eiko
E
Department of Nutritional Sciences, School of Nutritional Sciences, Nagoya University of Arts and Sciences, 57 Takenoyama, Iwasaki-cho, Nisshin, Aichi 470-0196, Japan. e-ina@nuas.ac.jp
Tsukahara
Takamitsu
T
Kaji
Kazuhiko
K
Eguchi
Ryoji
R
Kanazawa
Hiroaki
H
Hayashi
Hisayoshi
H
Suzuki
Yuichi
Y
D000818:Animals; D021461:Animals, Outbred Strains; D017209:Apoptosis; D053938:DNA Fragmentation; D015194:Diffusion Chambers, Culture; D020895:Enterocytes / Q000473:pathology*; D020287:In Situ Nick-End Labeling; D066298:In Vitro Techniques; D007413:Intestinal Mucosa / Q000473:pathology*; D007583:Jejunum / Q000473:pathology*; D008297:Male; D051379:Mice