Proteomic study via a non-gel based approach of meningococcal outer membrane vesicle vaccine obtained from strain CU385: a road map for discovering new antigens.
This work presents the results from a study of the protein composition of outer membrane vesicles from VA-MENGOC-BC (Finlay Institute, Cuba), an available vaccine against serogroup B Neisseria meningitidis. Proteins were identified by means of SCAPE, a 2DE-free method for proteome studies. More than one hundred proteins were detected by tandem liquid chromatographymass spectrometry analysis of fractions enriched in peptides devoid of histidine or arginine residues, providing a detailed description of the vaccine. A bioinformatic analysis of the identified components resulted in the identification of 31 outer membrane proteins and three conserved hypothetical proteins, allowing the cloning, expression, purification and immunological study of two of them (NMB0088 and NMB1796) as new antigens.
Center for Genetic Engineering and Biotechnology, Havana, Cuba.
Jeovanis
J
Gil
L Zaro H
LZ
Betancourt
Gretel
G
Sardiñas
Daniel
D
Yero
Olivia
O
Niebla
Maité
M
Delgado
Darien
D
García
Rolando
R
Pajón
Aniel
A
Sánchez
Luis J
LJ
González
Gabriel
G
Padrón
Concepción
C
Campa
Franklin
F
Sotolongo
Ramón
R
Barberó
Gerardo
G
Guillén
Luis
L
Herrera
Vladimir
V
Besada
D000942:Antigens, Bacterial / Q000032:analysis*; D001426:Bacterial Proteins / Q000032:analysis*; D002853:Chromatography, Liquid / Q000379:methods; D003462:Cuba; D006801:Humans; D022401:Meningococcal Vaccines / Q000737:chemistry*; D038541:Neisseria meningitidis, Serogroup B / Q000737:chemistry*; D020543:Proteome / Q000032:analysis*; D022142:Secretory Vesicles / Q000737:chemistry*; D053719:Tandem Mass Spectrometry / Q000379:methods
D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D022401:Meningococcal Vaccines; D020543:Proteome