Surface modification using a novel type I hydrophobin HGFI.
Surface wettability conversion with hydrophobins is important for its applications in biodevices. In this work, the application of a type I hydrophobin HGFI in surface wettability conversion on mica, glass, and poly(dimethylsiloxane) (PDMS) was investigated. X-ray photoelectron spectroscopy (XPS) and water-contact-angle (WCA) measurements indicated that HGFI modification could efficiently change the surface wettability. Data also showed that self-assembled HGFI had better stability than type II hydrophobin HFBI. Protein patterning and the following immunoassay illustrated that surface modification with HGFI should be a feasible strategy for biosensor device fabrication.
Hou
Sen
S
The Key Laboratory of Bioactive Materials, Ministry of Education, College of Life Science, Nankai University, Tianjin, 300071, China.
Li
Xinxin
X
Li
Xiaoyu
X
Feng
Xi-Zeng
XZ
Wang
Rui
R
Wang
Chen
C
Yu
Lei
L
Qiao
Ming-Qiang
MQ
D000538:Aluminum Silicates / Q000737:chemistry; D004129:Dimethylpolysiloxanes / Q000737:chemistry; D005656:Fungal Proteins / Q000737:chemistry*; D005898:Glass / Q000737:chemistry; D057927:Hydrophobic and Hydrophilic Interactions; D007118:Immunoassay; D013053:Spectrophotometry; D013499:Surface Properties; D014867:Water / Q000737:chemistry; D017155:Wettability; D014965:X-Rays
D000538:Aluminum Silicates; D004129:Dimethylpolysiloxanes; D005656:Fungal Proteins; D014867:Water; C011934:mica