[Role of oubain in regulation of growth of different tissues].
Using the method of organotypical cell culture, experimental data was obtained supporting the hypothesis that chelate complex ouabain-Ca2+ modulates the transducer function of Na+, K+- ATPase. Quantum-chemical calculations helped to elucidate two principally distinct modes for chelation of Ca2+ ions by ouabain molecule. It is predicted that ligang-receptor complex of ouabain-Ca2+--Na+,K+-ATPase is formed due to ion-ionic bonds. The forming of this complex switches on the transducer function of the sodium pump. Our data also show that removal of free Ca2+ ions from the cell culture media using EGTA has no effect on binding of ouabain with pumping control site of Na+K+-ATPase.
Kipenko
A V
AV
Penniiaĭnen
V A
VA
Lopatina
E V
EV
Rogachevskiĭ
I V
IV
Tsyrlin
V A
VA
Krylov
B V
BV
D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002316:Cardiotonic Agents / Q000494:pharmacology*; D002614:Chelating Agents / Q000494:pharmacology; D002642:Chick Embryo; D009924:Organ Culture Techniques; D009928:Organ Specificity / Q000187:drug effects; D010042:Ouabain / Q000494:pharmacology*; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism*
D002316:Cardiotonic Agents; D002614:Chelating Agents; D010042:Ouabain; D000254:Sodium-Potassium-Exchanging ATPase; D002118:Calcium