Molecular plant breeding: methodology and achievements.
The progress made in DNA marker technology has been remarkable and exciting in recent years. DNA markers have proved valuable tools in various analyses in plant breeding, for example, early generation selection, enrichment of complex F(1)s, choice of donor parent in backcrossing, recovery of recurrent parent genotype in backcrossing, linkage block analysis and selection. Other main areas of applications of molecular markers in plant breeding include germplasm characterization/fingerprinting, determining seed purity, systematic sampling of germplasm, and phylogenetic analysis. Molecular markers, thus, have proved powerful tools in replacing the bioassays and there are now many examples available to show the efficacy of such markers. We have illustrated some basic concepts and methodology of applying molecular markers for enhancing the selection efficiency in plant breeding. Some successful examples of product developments of molecular breeding have also been presented.
Varshney
Rajeev K
RK
International Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Patancheru 502 324, India.
Hoisington
Dave A
DA
Nayak
Spurthi N
SN
Graner
Andreas
A
D001947:Breeding / Q000379:methods*; D002874:Chromosome Mapping; D032621:DNA Shuffling / Q000379:methods; D018744:DNA, Plant / Q000235:genetics; D002523:Edible Grain / Q000235:genetics; D005819:Genetic Markers; D005838:Genotype; D015810:Linkage Disequilibrium; D010944:Plants / Q000235:genetics*; D011110:Polymorphism, Genetic; D040641:Quantitative Trait Loci
D018744:DNA, Plant; D005819:Genetic Markers