Nonenzymatic, self-elimination degradation mechanism of glutathione.
In the present in vitro studies, evidence is provided showing that glutathione (GSH) can undergo spontaneous, nonenzymatic auto-degradation. The initial cleavage of the Glu-Cys bond involves nucleophilic attack of the N-terminal amino group of GSH at the gamma-carbonyl side chain, followed by an unusual cleavage of the Cys-Gly amide bond that is complete within 3-5 weeks in the physiological pH range. These observations may be useful for the development of novel biodegradable polymers and releasable prodrugs for sustained and targeted drug delivery applications.
Deshmukh
Manjeet
M
Department of Pharmaceutics, Rutgers University, Piscataway, NJ 08854, USA.
Kutscher
Hilliard
H
Stein
Stanley
S
Sinko
Patrick
P
D002853:Chromatography, Liquid; D005978:Glutathione / Q000737:chemistry* / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry
D005978:Glutathione