Water saturation shift referencing (WASSR) for chemical exchange saturation transfer (CEST) experiments.
Chemical exchange saturation transfer (CEST) is a contrast mechanism that exploits exchange-based magnetization transfer (MT) between solute and water protons. CEST effects compete with direct water saturation and conventional MT processes, and generally can only be quantified through an asymmetry analysis of the water saturation spectrum (Z-spectrum) with respect to the water frequency, a process that is exquisitely sensitive to magnetic field inhomogeneities. Here it is shown that direct water saturation imaging allows measurement of the absolute water frequency in each voxel, allowing proper centering of Z-spectra on a voxel-by-voxel basis independently of spatial B(0) field variations. Optimal acquisition parameters for this "water saturation shift referencing" (WASSR) approach were estimated using Monte Carlo simulations and later confirmed experimentally. The optimal ratio of the WASSR sweep width to the linewidth of the direct saturation curve was found to be 3.3-4.0, requiring a sampling of 16-32 points. The frequency error was smaller than 1 Hz at signal-to-noise ratios of 40 or higher. The WASSR method was applied to study glycogen, where the chemical shift difference between the hydroxyl (OH) protons and bulk water protons at 3T is so small (0.75-1.25 ppm) that the CEST spectrum is inconclusive without proper referencing.
Kim
Mina
M
Russell H. Morgan Department of Radiology and Radiological Sciences, Neurology Section, Division of MR Research, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA.
Gillen
Joseph
J
Landman
Bennett A
BA
Zhou
Jinyuan
J
van Zijl
Peter C M
PC
D000328:Adult; D000465:Algorithms; D001834:Body Water / Q000378:metabolism*; D005260:Female; D006801:Humans; D007089:Image Enhancement / Q000379:methods; D007090:Image Interpretation, Computer-Assisted / Q000379:methods*; D008279:Magnetic Resonance Imaging / Q000379:methods*; D008297:Male; D018482:Muscle, Skeletal / Q000033:anatomy & histology* / Q000378:metabolism*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D014867:Water / Q000032:analysis*
D014867:Water