Separation and determination of cefotaxime enantiomers in injections by capillary zone electrophoresis.
Cefotaxime enantiomers have specific effects on Gram-negative bacteria. For quality control of cefotaxime it was necessary to establish a method for enantioseparation by capillary zone electrophoresis (CZE) using cyclodextrin (CD) as a chiral selector. The effects of various parameters on enantioseparation were studied. A fused silica capillary (40 cm effective length x 75 microm ID) was used. The cefotaxime enantiomers were separated on the baseline under conditions of 0.5 mmol/L CM-beta-CD, 75 mmol/L NaH2PO4 buffer at pH 7.0 using UV detection at 280 nm. Applied voltage and capillary temperature were 20 kV and 25 degrees C, respectively. Under these conditions for enantioseparation, linear calibration curves were obtained in the range 2 approximately 160 microg/mL. The limit of detection for both isomers was less than 0.5 microg/mL. The method was used for analysis of pharmaceutical preparations (dosage forms) of cefotaxime from various factories. A simple and specific CZE method was successfully demonstrated for the separation of cefotaxime enantiomers. The enantioseparation method should be established and this method should be used to control the quality of cefotaxime.
Department of Pharmacy, Lanzhou General Hospital, Lanzhou Command of PLA, Lanzhou 730050, China. Wangrong-69@163.com
Rong
R
Wang
Zheng-Ping
ZP
Jia
Jun-Jie
JJ
Fan
Jun
J
Ma
Xie
X
Hua
Qiang
Q
Zhang
Juan
J
Wang
D000900:Anti-Bacterial Agents / Q000032:analysis* / Q000302:isolation & purification; D002021:Buffers; D053801:Capillary Electrochromatography; D002439:Cefotaxime / Q000032:analysis* / Q000302:isolation & purification; D003505:Cyclodextrins / Q000737:chemistry; D005079:Excipients; D007267:Injections; D019999:Pharmaceutical Solutions / Q000032:analysis; D011786:Quality Control; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet; D013237:Stereoisomerism
D000900:Anti-Bacterial Agents; D002021:Buffers; D003505:Cyclodextrins; D005079:Excipients; D019999:Pharmaceutical Solutions; D002439:Cefotaxime