Biobased epicatechin conjugates protect erythrocytes and nontumoral cell lines from H2O2-induced oxidative stress.
This paper reports the study of the protective action of epicatechin and epicatechin derivatives, obtained by depolymerizing polymeric flavanols in the presence of cysteine or cysteamine, on red blood cells (RBC) and nontumoral cell lines challenged by exogenous H(2)O(2). The epicatechin derivatives showed more effective antioxidant properties than epicatechin. Among them, 4β-(2-aminoethylthio)epicatechin 3-O-gallate showed the highest antioxidant activity against three markers of oxidative stress: hemolysis, lipid peroxidation, and cytotoxicity. Furthermore, as this compound lacks the pyrogallol group on the condensed flavanic structure, it might be safer than other potent gallocatechin-type polyphenols. These findings indicate that these epicatechin derivatives, which are byproducts of the agro-food industry show potential for application in the food and drug industries.
Departament de Fisiologia, Facultat de Farmàcia, Universitat de Barcelona, 08028 Barcelona, Spain.
Vanessa
V
Ugartondo
Montserrat
M
Mitjans
Josep Lluís
JL
Torres
María Pilar
MP
Vinardell
D016475:3T3 Cells; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D002392:Catechin / Q000031:analogs & derivatives / Q000494:pharmacology*; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D004912:Erythrocytes / Q000187:drug effects* / Q000378:metabolism*; D006461:Hemolysis / Q000187:drug effects; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D015227:Lipid Peroxidation / Q000187:drug effects; D051379:Mice; D018384:Oxidative Stress / Q000187:drug effects*
D000975:Antioxidants; D002392:Catechin; D006861:Hydrogen Peroxide