Development of an enzyme-linked immunosorbent assay for the alpha-cyano pyrethroids multiresidue in Tai lake water.
A general and broad class selective competitive enzyme-linked immunosorbent assay (ELISA) for the detection of the alpha-cyano pyrethroids was developed. One class specific hapten, the 5-(3-benzylphenyl)-5-cyanopentanoic acid (CPBA-BE), was conjugated with BSA as an immunogen. Polyclonal antibodies, PY-antibodies, were generated. The assay with the most selectivity for the family alpha-cyano pyrethroids such as cyphenothrin was further optimized and tested for tolerance to cosolvent, pH, and ionic strength changes. The IC(50) values of the optimized immunoassay were 4.58 microg L(-1) for cyphenothrin, 5.62 microg L(-1) for fenpropathrin, 7.08 microg L(-1) for deltamethrin, 10.72 microg L(-1) for cypermethrin, 19.95 microg L(-1) for flucythrinate, and 28.18 microg L(-1) for esfenvalerate. The cross-reactivities of cyphenothrin, fenpropathrin, deltamethrin, cypermethrin, flucythrinate, and esfenvalerate were 100%, 81.49%, 64.68%, 42.72%, 22.96%, and 16.25%, respectively. The low detection limit (LDL) of this assay was 0.107 microg L(-1) for cyphenothrin. This method was used for the detection of the alpha-cyano pyrethroids in Tai lake water.
Hao
X L
XL
School of Food Science and Technology, Jiangnan University, Wuxi, JiangSu, 214000, China.
Kuang
H
H
Li
Y L
YL
Yuan
Y
Y
Peng
C F
CF
Chen
W
W
Wang
L B
LB
Xu
C L
CL
D002681:China; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005618:Fresh Water / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D007306:Insecticides / Q000032:analysis*; D009994:Osmolar Concentration; D010573:Pesticide Residues / Q000032:analysis*; D011722:Pyrethrins / Q000032:analysis*; D014874:Water Pollutants, Chemical / Q000032:analysis*
D007306:Insecticides; D010573:Pesticide Residues; D011722:Pyrethrins; D014874:Water Pollutants, Chemical; C051001:cyphenothrin