Chapter 6. Regulation of antibiotic production by bacterial hormones.
Antibiotic production is regulated by numerous signals, including the so-called bacterial hormones found in antibiotic producing organisms such as Streptomyces. These signals, the gamma-butyrolactones, are produced in very small quantities, which has hindered their structural elucidation and made it difficult to assess whether they are being produced. In this chapter, we describe a rapid small-scale extraction method from either solid or liquid cultures in scales of one plate or 50 ml of medium. Also described is a bioassay to detect the gamma-butyrolactones by determining either the production of pigmented antibiotic of Streptomyces coelicolor or kanamycin resistant growth on addition of the gamma-butyrolactones. We also describe some insights into the identification of the gamma-butyrolactone receptor and its targets and also the gel retardation conditions with three differently labeled probes.
Department of Microbial Physiology, GBB, University of Groningen, Haren, The Netherlands.
Nai-Hua
NH
Hsiao
Marco
M
Gottelt
Eriko
E
Takano
D015107:4-Butyrolactone / Q000096:biosynthesis* / Q000494:pharmacology; D024202:Electrophoretic Mobility Shift Assay; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects / Q000235:genetics; D007612:Kanamycin / Q000494:pharmacology; D011963:Receptors, GABA-A / Q000235:genetics / Q000378:metabolism; D013302:Streptomyces / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*
C037481:4-butyrolactone receptor; D011963:Receptors, GABA-A; D007612:Kanamycin; D015107:4-Butyrolactone