Determination of the Alternaria mycotoxin tenuazonic acid in cereals by high-performance liquid chromatography-electrospray ionization ion-trap multistage mass spectrometry after derivatization with 2,4-dinitrophenylhydrazine.
Tenuazonic acid (TA) is a major Alternaria mycotoxin. In the present work a novel approach for the detection of TA in cereals by liquid chromatography-ion-trap multistage mass spectrometry after derivatization with 2,4-dinitrophenylhydrazine is described. The product of the derivatization reaction and its major MS(2) fragments were characterised by Fourier transform-ion cyclotron resonance tandem mass spectrometry. Without preconcentration, the established method features a limit of detection of 10 microg/kg using 2g of sample in a rapid workup procedure. Accuracy, precision and linearity were evaluated in the working range of 50-5000 microg/kg. TA was detected in 13 and quantified in 3 out of 27 cereal samples obtained from a local supermarket, the average content being 49 microg/kg (highest incidence: 851+/-41 microg/kg).
Federal Institute for Materials Research and Testing, Division I.2, Berlin, Germany. david.siegel@bam.de
David
D
Siegel
Tatjana
T
Rasenko
Matthias
M
Koch
Irene
I
Nehls
D000528:Alternaria / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002523:Edible Grain / Q000737:chemistry*; D006835:Hydrazones / Q000737:chemistry; D010659:Phenylhydrazines / Q000737:chemistry*; D012015:Reference Standards; D015203:Reproducibility of Results; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D013720:Tenuazonic Acid / Q000032:analysis* / Q000737:chemistry
D006835:Hydrazones; D010659:Phenylhydrazines; C004787:2,4-dinitrophenylhydrazine; D013720:Tenuazonic Acid