A plant 5S ribosomal RNA mimic regulates alternative splicing of transcription factor IIIA pre-mRNAs.
Transcription factor IIIA (TFIIIA) is required for eukaryotic synthesis of 5S ribosomal RNA by RNA polymerase III. Here we report the discovery of a structured RNA element with clear resemblance to 5S rRNA that is conserved within TFIIIA precursor mRNAs from diverse plant lineages. TFIIIA protein expression is controlled by alternative splicing of the exon containing the plant 5S rRNA mimic (P5SM). P5SM triggers exon skipping upon binding of ribosomal protein L5, a natural partner of 5S rRNA, which demonstrates the functional adaptation of its structural mimicry. As the exon-skipped splice product encodes full-length TFIIIA protein, these results reveal a ribosomal protein-mRNA interaction that is involved in 5S rRNA synthesis and has implications for cross-coordination of ribosomal components. This study also provides insight into the origin and function of a newfound class of structured RNA that regulates alternative splicing.
Hammond
Ming C
MC
Department of Molecular, Cellular and Developmental Biology, Yale University, New Haven, Connecticut, USA.
Wachter
Andreas
A
Breaker
Ronald R
RR
D017398:Alternative Splicing / Q000235:genetics*; D001483:Base Sequence; D017124:Conserved Sequence; D005091:Exons / Q000235:genetics; D018506:Gene Expression Regulation, Plant; D008957:Models, Genetic; D018716:Molecular Mimicry; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D009154:Mutation / Q000235:genetics; D009690:Nucleic Acid Conformation; D010944:Plants / Q000235:genetics* / Q000378:metabolism*; D011485:Protein Binding; D012322:RNA Precursors / Q000235:genetics*; D018749:RNA, Plant / Q000737:chemistry / Q000235:genetics; D012341:RNA, Ribosomal, 5S / Q000737:chemistry / Q000235:genetics*; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D012269:Ribosomal Proteins / Q000378:metabolism; D035582:Transcription Factor TFIIIA / Q000235:genetics* / Q000378:metabolism
D012322:RNA Precursors; D018749:RNA, Plant; D012341:RNA, Ribosomal, 5S; D011993:Recombinant Fusion Proteins; D012269:Ribosomal Proteins; D035582:Transcription Factor TFIIIA; C029742:ribosomal protein L5