Comparative study of the constitution and chiroptical properties of emissive terbium and europium complexes with a common tetraazatriphenylene sensitiser; the nature of the sensitiser determines quenching sensitivity and cellular uptake.
Six pairs of Eu(III) and Tb(III) complexes of macrocyclic ligands, incorporating a common tetraazatriphenylene sensitiser, have been examined in terms of their solution structure, sensitivity to excited state quenching, protein affinity, cell toxicity and preliminary cell localisation profiles. A complex with three (S)-phenylalanine-derived ligating groups possesses distinctive 1H NMR, Eu emission and circularly polarised emission properties, consistent with a unique A-configuration in the 9-coordinate complex, where an amide carbonyl group occupies the capping position of the coordination polyhedron. Each complex possesses similar sensitivity to quenching by ascorbate, urate and iodide, has similar toxicity behaviour and shows a common intracellular localisation profile that is consistent with compartmentalisation in lysosomes or late endosomes. Such behaviour accords with the hypothesis that it is the nature of the sensitising moiety that determines each of these properties.
Department of Chemistry, Durham University, South Road, Durham, DH1 3LE, UK.
Elizabeth J
EJ
New
David
D
Parker
Robert D
RD
Peacock
D000818:Animals; D001372:Aza Compounds / Q000737:chemistry*; D016466:CHO Cells; D002911:Chrysenes / Q000737:chemistry*; D006224:Cricetinae; D003412:Cricetulus; D005063:Europium / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D051379:Mice; D041681:NIH 3T3 Cells; D013725:Terbium / Q000737:chemistry*
D001372:Aza Compounds; D002911:Chrysenes; C533582:azatriphenylene; D013725:Terbium; D005063:Europium