Assays for transcription factor activity.
Transcription factors interact at promoters to modulate the transcription of genes. This chapter describes three in vitro methods that can be used to monitor their activity: transcript assays, abortive initiation assays, and potassium permanganate footprinting. These techniques have been developed using bacterial systems, and can be used to study the kinetics of transcription initiation, and hence to unravel regulatory mechanisms.
Browning
Douglas
D
School of Biosciences, The University of Birmingham, Edgbaston, Birmingham, B15 2TT, UK.
Savery
Nigel
N
Kolb
Annie
A
Busby
Stephen
S
D001681:Biological Assay / Q000379:methods*; D002373:Cyclic AMP Receptor Protein / Q000378:metabolism; D018983:DNA Footprinting; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D010957:Plasmids / Q000235:genetics; D011196:Potassium Permanganate; D011401:Promoter Regions, Genetic / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D014157:Transcription Factors / Q000378:metabolism*; D014158:Transcription, Genetic; D015533:Transcriptional Activation / Q000235:genetics
D002373:Cyclic AMP Receptor Protein; D012333:RNA, Messenger; D014157:Transcription Factors; D011196:Potassium Permanganate; D012321:DNA-Directed RNA Polymerases