[Lipid transfer to HDl in women with type 1 diabetes].
BACKGROUND
People with type 1 diabetes mellitus (T1DM) have an increased risk of cardiovascular disease and may still have a normal lipid profile. In order to clarify whether normal HDL cholesterol levels may conceal defects in HDL function, we have studied the transfer of lipids to HDL in T1DM.


METHODS
Twenty-one young women with T1DM were compared with 21 non-diabetic women. Nanoemulsion preparations were used as lipid donor to HDL: one labeled with (3)H-triglycerides and 14C-free cholesterol and the other with (3)H-cholesteryl esters and 14C-phospholipids. These preparations were incubated with plasma samples for 1h. After chemical precipitation, the supernatant containing HDL was counted for radioactivity.


RESULTS
No difference in transfer was observed to nanoemulsion HDL from cholesteryl esters, triglycerides, free cholesterol and phospholipids.


CONCLUSIONS
Simultaneous lipid transfer to HDL was not affected in T1DM patients. This suggests that the disease does not alter lipoprotein composition and transfer protein action in such way as to disturb HDL metabolism.
Feitosa
Alina Coutinho Rodrigues
AC
Laboratório de Metabolismo de Lípides do instituto do Coração, Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo. alinafeitosa@yahoo.com.br
Maranhão
Raul Cavalcante
RC
Feitosa Filho
Gilson Soares
GS
Wajchenberg
Bernardo Léo
BL
D000328:Adult; D001692:Biological Transport / Q000502:physiology; D002352:Carrier Proteins / Q000378:metabolism*; D016022:Case-Control Studies; D002788:Cholesterol Esters / Q000008:administration & dosage / Q000097:blood / Q000493:pharmacokinetics; D003922:Diabetes Mellitus, Type 1 / Q000378:metabolism*; D005260:Female; D006801:Humans; D008055:Lipids / Q000008:administration & dosage* / Q000097:blood / Q000493:pharmacokinetics; D008075:Lipoproteins, HDL / Q000737:chemistry / Q000378:metabolism / Q000648:ultrastructure*; D053758:Nanoparticles / Q000008:administration & dosage*; D010743:Phospholipids / Q000008:administration & dosage / Q000097:blood / Q000493:pharmacokinetics; D018709:Statistics, Nonparametric; D014280:Triglycerides / Q000008:administration & dosage / Q000097:blood / Q000493:pharmacokinetics; D055815:Young Adult
D002352:Carrier Proteins; D002788:Cholesterol Esters; D008055:Lipids; D008075:Lipoproteins, HDL; D010743:Phospholipids; D014280:Triglycerides; C030363:lipid transfer protein