Development of CRTEIL and CETRIZ, Cre-loxP-based systems, which allow change of expression of red to green or green to red fluorescence upon transfection with a cre-expression vector.
We developed Cre-loxP-based systems, termed CRTEIL and CETRIZ, which allow gene switching in a noninvasive manner. Single transfection with pCRTEIL resulted in predominant expression of red fluorescence. Cotransfection with pCRTEIL and Cre-expression plasmid (pCAG/NCre) caused switching from red to green fluorescence. Similarly, cotransfection with pCETRIZ and pCAG/NCre resulted in change of green to red fluorescence. These noninvasive systems will be useful in cell lineage analysis, since descendants of cells exhibiting newly activated gene expression can be continuously monitored in noninvasive fashion.
Ohtsuka
Masato
M
Division of Basic Molecular Science and Molecular Medicine, School of Medicine, Tokai University, Bohseidai, Isehara, Kanagawa 259-1193, Japan. masasato@ms.kagoshima-u.ac.jp
Warita
Takayuki
T
Sakurai
Takayuki
T
Watanabe
Satoshi
S
Inoko
Hidetoshi
H
Sato
Masahiro
M
D000818:Animals; D017101:Bacteriophage P1 / Q000235:genetics; D003001:Cloning, Molecular; D005456:Fluorescent Dyes / Q000378:metabolism; D015870:Gene Expression; D005822:Genetic Vectors / Q000378:metabolism; D049452:Green Fluorescent Proteins / Q000235:genetics* / Q000378:metabolism; D019426:Integrases / Q000235:genetics* / Q000378:metabolism; D049408:Luminescent Agents / Q000378:metabolism; D008163:Luminescent Measurements; D008164:Luminescent Proteins / Q000235:genetics* / Q000378:metabolism; D051379:Mice; D041681:NIH 3T3 Cells; D011995:Recombination, Genetic; D014162:Transfection; D019076:Transgenes
D005456:Fluorescent Dyes; D049408:Luminescent Agents; D008164:Luminescent Proteins; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins; C045073:Cre recombinase; D019426:Integrases