Neudesin, an extracellular heme-binding protein, suppresses adipogenesis in 3T3-L1 cells via the MAPK cascade.
Adult mice abundantly express neudesin, an extracellular heme-binding protein with neurotrophic activity, in white adipose tissues. At the early stage of adipocyte differentiation during adipogenesis, however, the expression of neudesin decreased transiently. Neudesin-hemin significantly suppressed adipogenesis in 3T3-L1 cells. The knockdown of neudesin by RNA interference markedly promoted adipogenesis in 3T3-L1 cells and decreased MAPK activation during adipocyte differentiation. The addition or knockdown of neudesin affected the expression of C/EBPalpha and PPARgamma but not of C/EBPbeta. These findings suggest that neudesin plays a critical role in the early stage of adipocyte differentiation in which C/EBPbeta induces PPARgamma and C/EBPalpha expressions, by controlling the MAPK pathway.
Laboratory of Applied Pharmacology, Faculty of Pharmacy, Chiba Institute of Science, 15-8 Shiomi-cho, Choshi, Chiba 288-0025, Japan. ikimura@pharm.kyoto-u.ac.jp
Ikuo
I
Kimura
Morichika
M
Konishi
Toshiyuki
T
Asaki
Naoko
N
Furukawa
Kiyotaka
K
Ukai
Masahiro
M
Mori
Akira
A
Hirasawa
Gozoh
G
Tsujimoto
Mitsuhiro
M
Ohta
Nobuyuki
N
Itoh
Masafumi
M
Fujimoto
D041721:3T3-L1 Cells; D017667:Adipocytes / Q000201:enzymology / Q000502:physiology*; D050156:Adipogenesis; D000818:Animals; D049109:Cell Proliferation; D055785:Gene Knockdown Techniques; D020935:MAP Kinase Signaling System; D051379:Mice; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000502:physiology*; D034622:RNA Interference
D009419:Nerve Tissue Proteins; C500817:neudesin protein, mouse