Discovery of novel benzene 1,3-dicarboxylic acid inhibitors of bacterial MurD and MurE ligases by structure-based virtual screening approach.
The peptidoglycan biosynthetic pathway provides an array of potential targets for antibacterial drug design, attractive especially with respect to selective toxicity. Within this pathway, the members of the Mur ligase family are considered as promising emerging targets for novel antibacterial drug design. Based on the available MurD crystal structures co-crystallised with N-sulfonyl glutamic acid inhibitors, a virtual screening campaign was performed, combining three-dimensional structure-based pharmacophores and molecular docking calculations. A novel class of glutamic acid surrogates-benzene 1,3-dicarboxylic acid derivatives-were identified and compounds 14 and 16 found to possess dual MurD and MurE inhibitory activity.
National Institute of Chemistry, Hajdrihova, Ljubljana, Slovenia.
Andrej
A
Perdih
Andreja
A
Kovac
Gerhard
G
Wolber
Didier
D
Blanot
Stanislav
S
Gobec
Tom
T
Solmajer
D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000494:pharmacology; D001555:Benzene Derivatives / Q000737:chemistry* / Q000494:pharmacology; D003198:Computer Simulation; D018360:Crystallography, X-Ray; D003998:Dicarboxylic Acids / Q000737:chemistry* / Q000494:pharmacology; D055808:Drug Discovery; D004791:Enzyme Inhibitors / Q000737:chemistry* / Q000494:pharmacology; D010453:Peptide Synthases / Q000037:antagonists & inhibitors* / Q000378:metabolism; D013329:Structure-Activity Relationship
D000900:Anti-Bacterial Agents; D001555:Benzene Derivatives; D003998:Dicarboxylic Acids; D004791:Enzyme Inhibitors; D010453:Peptide Synthases; C049448:UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-lysine ligase; C053379:UDP-N-acetylmuramoylalanine-D-glutamate ligase