Ultrahigh spacing tissue arrays for screening hybridoma by immunohistochemistry.
We present a technology to make ultrahigh spacing tissue arrays (USTA) which facilitates screening hybridoma by immunohistochemistry, results in easier performance of staining and image analysis, minimal sample mixed-up and less bookkeeping efforts. 32 array cores are designated a 4 x 8 microarray, leaving 4 mm spacing between two adjacent array elements. After separated each array spot with PAP pen, one USTA is sufficient for testing of supernatants from 32 different hybridomas.
Song
Chaojun
C
Department of Immunology, Fourth Military Medical University, 17 Changle West Road, Xi'an 710032, PR China.
Wang
Fuli
F
Jin
Liang
L
Chen
Lihua
L
Qin
Weijun
W
Yang
Angang
A
Yang
Kun
K
Jin
Boquan
B
D000818:Animals; D006801:Humans; D006825:Hybridomas / Q000166:cytology*; D007150:Immunohistochemistry / Q000379:methods*; D051379:Mice; D046228:Microarray Analysis; D016610:Tissue Embedding / Q000379:methods*