Manassantin A and B from Saururus chinensis inhibiting cellular melanin production.
Hyperpigmentation disorders such as freckles and senile lentigines in the skin are associated with abnormal accumulation of melanin pigments. In this study, two lignan constituents were isolated from Saururus chinensis Baill (Saururaceae) as inhibitors of cellular melanin production by bioassay-guided fractionations. The active constituents were manassantin A and B that dose-dependently inhibited melanin production in alpha-melanocyte stimulating hormone (alpha-MSH)-activated melanoma B16 cells with IC(50) values of 13 nm and 8 nm, respectively. Arbutin as a positive control exhibited an IC(50) value of 96 microm on alpha-MSH-induced melanin production. Further, manassantin A inhibited forskolin- or 3-isobutyl-1-methylxanthine (IBMX)-induced melanin production with IC(50) values of 14 nm or 12 nm, respectively. Manassantin A decreased cellular amounts of IBMX-inducible tyrosinase protein but could not affect the catalytic activity of cell-free tyrosinase, a key enzyme in the biosynthetic pathway of melanin pigments. Finally, this study could provide a pharmacological potential of S. chinensis in hyperpigmentation disorders.
Seo
Chang-Seob
CS
College of Pharmacy, Yeungnam University, Gyongsan 712-749, Korea.
Lee
Won-Hee
WH
Chung
Hee-Wook
HW
Chang
Eun Ju
EJ
Lee
Seung Ho
SH
Jahng
Yurngdong
Y
Hwang
Bang Yeon
BY
Son
Jong-Keun
JK
Han
Sang-Bae
SB
Kim
Youngsoo
Y
D000818:Animals; D001104:Arbutin / Q000494:pharmacology; D045744:Cell Line, Tumor; D005663:Furans / Q000494:pharmacology*; D017495:Hyperpigmentation / Q000188:drug therapy; D020128:Inhibitory Concentration 50; D017705:Lignans / Q000494:pharmacology*; D008543:Melanins / Q000037:antagonists & inhibitors* / Q000096:biosynthesis; D008546:Melanoma, Experimental / Q000378:metabolism; D015394:Molecular Structure; D014442:Monophenol Monooxygenase / Q000378:metabolism; D010858:Pigmentation / Q000187:drug effects*; D032134:Saururaceae / Q000737:chemistry*
D005663:Furans; D017705:Lignans; D008543:Melanins; C482885:manassantin B; C054895:manassantin A; D001104:Arbutin; D014442:Monophenol Monooxygenase