Effects of hyperglycemia and aging on the healing of colonic anastomoses in rats.
BACKGROUND
Despite the significant advances in the knowledge of the healing process, there is a limited number of studies demonstrating the relationships of this healing with ageing and elevated levels of glycemia.


OBJECTIVE
To evaluate the effects of ageing and hyperglycemia on the healing of colonic anastomosis.


METHODS
138 young and old male rats were utilized. Some of them were normoglycemic and others had hyperglycemia induced by streptozocin (50mg/kg). They were maintained under control for 90 days. They were then submitted to a termino-terminal anastomosis in the left colon. On the third, seventh and fourteenth days after surgery, their resistance was evaluated and a histopathological study of the anastomosis was carried out.


RESULTS
Gain in resistance was similar for both groups. The additive effect of age with hyperglycemia made a significant difference to the collagen I (p<0.001), III (p=0.022) and total (p<0.001). Among the old animals, the glycemia was a determining factor for the occurrence of a significant difference in total collagen (p=0.029) and collagen I (p=0.013). Among the normoglycemics, age determined a lower density of collagen I (p=0.002).


CONCLUSIONS
There is delayed collagen synthesis and maturation of the scars of older animals, a situation that becomes more serious in older hyperglycemic animals, but insufficient to adversely affect the gain in resistance.
Scientific Methodology, PUCPR, Department of Surgery, Federal University of Parana, Brazil. biondo@avalon.sul.com.br
Maria de Lourdes Pessole
Mde L
Biondo-Simões
Rachel
R
Biondo-Simões
Sergio Ossamu
SO
Ioshii
Daniel Sindelar
DS
Barczak
Mariana Rocha
MR
Tetilla
D000375:Aging / Q000502:physiology*; D000704:Analysis of Variance; D000714:Anastomosis, Surgical; D000818:Animals; D003094:Collagen / Q000494:pharmacology*; D003106:Colon / Q000601:surgery*; D004195:Disease Models, Animal; D006943:Hyperglycemia / Q000503:physiopathology*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D018709:Statistics, Nonparametric; D014945:Wound Healing / Q000502:physiology*
D003094:Collagen