[Study of encapsulation of suicide gene in PEG-PEI/Fe3O4 nano-magnetic fluid and its characteristics in vitro].
OBJECTIVE
To prepare PEG-PEI Fe(3)O(4) nano-magnetic fluid and invest its characteristics.


METHODS
PEG-PEI Fe(3)O(4) nano-magnetic fluid was prepared by chemical co-deposition as gene delivery vector. Then DNA of plasmid pAFP-TK was wrapped into PEG-PEI Fe(3)O(4) nano-magnetic fluid. At the same time, we invest its characteristics with MTT assay.


RESULTS
PEG-PEI Fe(3)O(4) nano-magnetic fluid-DNA complexes could protect the DNA from degradation of DNaseI, serum and sonication. AFP positive cells transfected by pAFP-TK were highly sensitive to the GCV treatment, and the survival of cells declined obviously. HSV-TK/GCV suicide gene therapy system had direct cell toxicity.


CONCLUSIONS
This new target strategy of pAFP-TK/GCV suicide gene therapy system in which PEG-PEI Fe(3)O(4) nano-magnetic fluid are used as gene delivery vectors explores a promising area for AFP positive HCC and associated carcinoma therapy.
Xue
Dun
D
National Hepatobiliary & Enteric Surgery Research Center, Central South University, Changsha 410008, China. pengjian1698@yahoo.cn
He
Jian-tai
JT
Peng
Jian
J
Zhao
Jin-feng
JF
Zhang
Yang-de
YD
D016475:3T3 Cells; D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D005290:Ferric Compounds / Q000737:chemistry; D015774:Ganciclovir / Q000494:pharmacology*; D041722:Genes, Transgenic, Suicide; D005822:Genetic Vectors / Q000737:chemistry / Q000235:genetics; D006367:HeLa Cells; D006801:Humans; D007097:Imines / Q000737:chemistry; D008280:Magnetics; D051379:Mice; D036103:Nanotechnology / Q000379:methods; D011092:Polyethylene Glycols / Q000737:chemistry; D011095:Polyethylenes / Q000737:chemistry; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D018139:Simplexvirus / Q000201:enzymology / Q000235:genetics; D013937:Thymidine Kinase / Q000235:genetics / Q000378:metabolism*; D014162:Transfection; D000509:alpha-Fetoproteins / Q000235:genetics / Q000378:metabolism*
D000998:Antiviral Agents; D005290:Ferric Compounds; D007097:Imines; D011095:Polyethylenes; D011993:Recombinant Fusion Proteins; D000509:alpha-Fetoproteins; C505405:poly(ethylene imine); C000499:ferric oxide; D011092:Polyethylene Glycols; D013937:Thymidine Kinase; D015774:Ganciclovir