HPLC with pulsed amperometric detection for sorbitol as a biomarker for diabetic neuropathy.
This study describes the optimal analytical conditions for sorbitol analysis by a high-performance anion-exchange chromatography-pulsed amperometric detection method. Its clinical utility as a diagnostic tool was established by measuring sorbitol in the sciatic nerves or salivary glands of diabetes mellitus-induced mice. Sorbitol was completely separated from other monosaccharides on an anion-exchange column with 100mM NaOH as eluent. The limit of detection (S/N=3) and limit of quantification (S/N=10) were 0.03 ng (3 ng/g) and 0.10 ng (10 ng/g), respectively. The linear dynamic range was 0.01-50.0 microg/g (r(2)=0.9997 and 0.9989 for sciatic nerves and salivary glands, respectively), and the mean recoveries for intra- or inter-day assays were in the range of 98.5-103.9%. This method easily identified diabetic and normal groups, making it a practical procedure for the rapid screening of diabetic neuropathy.
Department of Oriental Pharmaceutical Sciences, Kyung Hee East-West Pharmaceutical Research Institute, College of Pharmacy, Kyung Hee University, Dongdaemoon-gu, Seoul, South Korea.
Hee-Jung
HJ
Sim
Ji-Seon
JS
Jeong
Ha-Jeong
HJ
Kwon
Tong Ho
TH
Kang
Hye Min
HM
Park
Yong-Moon
YM
Lee
Sun Yeou
SY
Kim
Seon-Pyo
SP
Hong
D000818:Animals; D015415:Biomarkers / Q000032:analysis; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003929:Diabetic Neuropathies / Q000175:diagnosis*; D006801:Humans; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D012469:Salivary Glands / Q000737:chemistry; D012584:Sciatic Nerve / Q000737:chemistry; D013012:Sorbitol / Q000032:analysis*
D015415:Biomarkers; D013012:Sorbitol