A lectin from Sesbania aculeata (Dhaincha) roots and its possible function.
A lectin was isolated from the roots of Sesbania aculeata. This is a glucose specific lectin having 39 kDa subunit molecular weight. The expression of this lectin was found to be developmentally regulated and observed to be the highest in the second week. The lectin was purified by affinity chromatography using Sephadex G-50 and found to have 28% homology with Arabidopsis thaliana lectin-like protein (accession No. CAA62665). The lectin binds with lipopolysaccharide isolated from different rhizobial strains indicating the plants interaction with multiple rhizobial species.
Institute of Genomics and Integrative Biology, Delhi University Campus, Mall Road, Delhi 110007, India.
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S
Biswas
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Saroha
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HR
Das
D000595:Amino Acid Sequence; D000818:Animals; D002241:Carbohydrates / Q000032:analysis; D002846:Chromatography, Affinity; D015180:Electrophoresis, Gel, Two-Dimensional; D004591:Electrophoresis, Polyacrylamide Gel; D006386:Hemagglutination Tests; D008070:Lipopolysaccharides / Q000737:chemistry / Q000378:metabolism; D008969:Molecular Sequence Data; D008970:Molecular Weight; D037121:Plant Lectins / Q000737:chemistry / Q000378:metabolism* / Q000502:physiology; D018517:Plant Roots / Q000378:metabolism*; D011485:Protein Binding; D011817:Rabbits; D049931:Sesbania / Q000378:metabolism*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D002241:Carbohydrates; D008070:Lipopolysaccharides; D037121:Plant Lectins