Lipid-soluble components of honeybee-collected pollen exert antiallergic effect by inhibiting IgE-mediated mast cell activation in vivo.
It was shown previously that bee-collected pollen (bee pollen, BP), inhibited in vitro murine mast cell activation. This study further analysed the antiallergic effect of BP in vivo by measuring cutaneous mast cell activation using a passive cutaneous anaphylaxis reaction. Daily oral administration of BP to mice, dose-dependently reduced the cutaneous mast cell activation elicited by IgE and specific antigens. Administration of BP also reduced the plasma concentration of malondialdehyde (MDA), an indicator of lipid peroxidation. The inhibitory effect of BP was mostly in a lipid- but not in water-soluble fraction. The HPLC analysis of isoflavones in BP revealed that genistein was a major isoflavone. However, administration of genistein alone at the concentration found in BP, did not show an inhibitory effect as observed in whole BP, suggesting that component(s) other than genistein would be responsible for the inhibitory effect of BP. These results first reveal that lipid-soluble components of BP exert an antiallergic action by inhibiting the FcåRI-mediated cutaneous mast cell activation.
Atopy (Allergy) Research Center, Juntendo University School of Medicine, 2-1-1 Hongo, Bunkyo-ku, Tokyo 113-8421, Japan.
Yasuko
Y
Ishikawa
Tomoko
T
Tokura
Hiroko
H
Ushio
François
F
Niyonsaba
Yuji
Y
Yamamoto
Tadahiro
T
Tadokoro
Hideoki
H
Ogawa
Ko
K
Okumura
D000818:Animals; D018926:Anti-Allergic Agents / Q000494:pharmacology*; D001516:Bees; D019833:Genistein / Q000494:pharmacology; D007073:Immunoglobulin E / Q000276:immunology*; D015227:Lipid Peroxidation; D008297:Male; D008315:Malondialdehyde / Q000097:blood; D008407:Mast Cells / Q000187:drug effects* / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D010323:Passive Cutaneous Anaphylaxis; D010936:Plant Extracts / Q000494:pharmacology; D011058:Pollen / Q000276:immunology*
D018926:Anti-Allergic Agents; D010936:Plant Extracts; D007073:Immunoglobulin E; D008315:Malondialdehyde; D019833:Genistein