[Effect of the liver X receptor agonist on LPS-induced inflammatory response associated factor IRAK-4 and NF-kappaB].
OBJECTIVE
To investigate the effect of the activated LXR on the expression of IRAK-4 and NF-kappaB in the inflammatory response which was induced by LPS in Kupffer cells.


METHODS
The Kuppfer cells were isolated from male Kunming mice by collagen perfusion in situ. These cells were divided into four groups: normal control group, LPS treatment group, LXR agonist T0901317 treatment group, LPS and T0901317 combined treatment group. The expression of LXR, IRAK-4 and NF-kappaB gene and their protein levels were detected by real time-PCR and Western blot, and the activity of NF-kappaB was observed by EMSA.


RESULTS
The levels of LXR mRNA and protein reached the highest in T0901317 group but the lowest in LPS group. The levels of IRAK4 and NF-kappaB mRNA and protein in the combined-treatment group were evidently lower than those in LPS group. The activity of NF-kappaB reached the highest in LPS group but it was lower in the combined-treatment group and T0901317 group.


CONCLUSIONS
LXR agonists can effectively up-regulate the level of LXR gene and protein and down-regulate the levels of IRAK4 and NF-kappaB mRNA and protein expression. They can also inhibit activity of NF-kappaB.
Department of Hepatobiliary Surgery, The Second Affiliated Hospital of Chongqing Medical University, Chongqing 400010, China. wdlz129@163.com
Ding
D
Wang
Chun-mu
CM
Miao
Jian-ping
JP
Gong
D000818:Animals; D015153:Blotting, Western; D002478:Cells, Cultured; D004357:Drug Synergism; D006845:Hydrocarbons, Fluorinated / Q000494:pharmacology; D053592:Interleukin-1 Receptor-Associated Kinases / Q000235:genetics / Q000378:metabolism*; D007728:Kupffer Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D008070:Lipopolysaccharides / Q000494:pharmacology*; D000071518:Liver X Receptors; D008297:Male; D016328:NF-kappa B / Q000235:genetics / Q000378:metabolism*; D057093:Orphan Nuclear Receptors / Q000819:agonists* / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013449:Sulfonamides / Q000494:pharmacology; D005466:Fluorocarbons; D000096926:Benzenesulfonamides
D006845:Hydrocarbons, Fluorinated; D053592:Interleukin-1 Receptor-Associated Kinases; D008070:Lipopolysaccharides; D000071518:Liver X Receptors; D016328:NF-kappa B; D057093:Orphan Nuclear Receptors; D012333:RNA, Messenger; D013449:Sulfonamides; C423915:T0901317; D005466:Fluorocarbons; D000096926:Benzenesulfonamides