Flow cytometric investigation of neutrophil oxidative burst and apoptosis in physiological and pathological situations.
Flow cytometric analysis provides a rapid screen for abnormalities of polymorphonuclear neutrophils (PMN) function and reflect their behavior in vivo more accurately. This review summarizes the major fluorescent probes used to study PMN oxidative burst and apoptosis using flow cytometry (FCM). We also provide examples of FCM studies in physiological and pathological situations, illustrating the advantages of FCM for assessment of PMN oxidative burst and PMN apoptosis. These data point to the role of FCM in detecting primary immunodeficiencies such as IRAK4 deficiency and support the use of the assessment of the PMN oxidative burst for routine testing in patients with bacterial infections. We also demonstrate the utility of whole-blood analysis using FCM for a better understanding of PMN functionality, i.e., tuning PMN responses to inflammatory stimuli. Finally, FCM permits a simultaneous analysis of phenotypic, functional and morphometric parameters assessing whole-blood PMN apoptosis, in particular in response to Toll-like receptor agonists and during simian immunodeficiency virus infection.
Centre de Recherche des Cordeliers, Université Pierre et Marie Curie, Paris, France. carole.elbim@crc.jussieu.fr
Carole
C
Elbim
Gérard
G
Lizard
D000276:Adjuvants, Immunologic / Q000494:pharmacology; D000818:Animals; D017209:Apoptosis; D005434:Flow Cytometry; D006801:Humans; D007093:Imidazoles / Q000494:pharmacology; D053592:Interleukin-1 Receptor-Associated Kinases / Q000172:deficiency; D008070:Lipopolysaccharides / Q000494:pharmacology; D019255:NADPH Oxidases / Q000378:metabolism; D009504:Neutrophils / Q000187:drug effects / Q000276:immunology*; D009838:Oligodeoxyribonucleotides / Q000494:pharmacology; D017382:Reactive Oxygen Species / Q000378:metabolism; D016897:Respiratory Burst / Q000502:physiology*; D016097:Simian Acquired Immunodeficiency Syndrome / Q000276:immunology; D051193:Toll-Like Receptors / Q000819:agonists / Q000378:metabolism*
D000276:Adjuvants, Immunologic; C408982:CPG-oligonucleotide; D007093:Imidazoles; D008070:Lipopolysaccharides; D009838:Oligodeoxyribonucleotides; D017382:Reactive Oxygen Species; D051193:Toll-Like Receptors; D019255:NADPH Oxidases; C507773:IRAK4 protein, human; D053592:Interleukin-1 Receptor-Associated Kinases; C402365:resiquimod