Increased glycated calmodulin in the submandibular salivary glands of streptozotocin-induced diabetic rats.
Non-enzymatic glycosylation, a post translational protein modification may be implicated in the diabetes complications. Calmodulin is an important calcium binding protein that complexed with Ca(2+) may be implicated in salivary gland secretory process. Glycated calmodulin has shown to be less effective in binding calcium. The aim of this study was to determine whether the concentration of glycated-calmodulin may be elevated in the submandibular salivary glands of streptozotocin-induced diabetic rats. Diabetes was induced by an intraperitoneal injection of spreptozotocin, and hyperglycemia was confirmed 72 h after injection using a glucosimeter. Thirty days after the induction of diabetes, submandibular salivary glands were used for the analysis of glycated and non-glycated calmodulin, using a glycogel B columns for separation. Glycated and non-glycated calmodulin were assayed by an enzymatic method and by ELISA. The overall concentration of CaM (non-glycated + glycated) in induced diabetic rats was significantly lower than in controls (p < 0.05). The concentration of non-glycated CaM in controls was significantly higher than in experimental group (p < 0.05), while the concentration of glycated calmodulin between these groups was statistically similar (p > 0.05).
Oral Biology Research Center, Dental Materials Department, School of Dentistry, University of São Paulo, Brazil.
José
J
Nicolau
Douglas Nesadal
DN
de Souza
Marcela
M
Carrilho
D000818:Animals; D002147:Calmodulin / Q000302:isolation & purification / Q000378:metabolism*; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism*; D006031:Glycosylation; D011499:Protein Processing, Post-Translational; D051381:Rats; D017208:Rats, Wistar; D013363:Submandibular Gland / Q000378:metabolism*; D013997:Time Factors
D002147:Calmodulin