Expression of stress-related genes in tomato plants exposed to arsenic and chromium in nutrient solution.
The molecular responses of hydroponically cultivated tomato plants to As(V) or Cr(VI) were assessed by transcript accumulation analysis of genes coding for products potentially involved in heavy metal tolerance. A quantitative real-time PCR experiment was performed with Hsp90-1, MT2- and GR1-like protein genes using RNA isolated from tomato roots or shoots treated for 24h with As(V) or Cr(VI) at concentrations ranging from 80 to 640 microM. Both transient metallic treatments induced Hsp90-1 transcript accumulation in tomato plants. MT2- and GR1-like transcripts accumulated in tomato roots treated with As(V) but were only slightly affected by Cr(VI) treatment. Tomatoes showed phenotypic symptoms to heavy metal toxicity when plants were exposed to Cr(VI) but not As(V). Plant lethality was observed at 1280 microM Cr(VI), indicating that tomatoes were more tolerant to As than Cr stress under the experimental conditions used here.
Laboratoire de Physiologie et Génétique végétales, ERTAC-UMR INRA, Université Blaise Pascal-Clermont II, Campus universitaire des Cézeaux, 24, avenue des Landais, 63177 Aubière cedex, France. Pascale.GOUPIL@univ-bpclermont.fr
Pascale
P
Goupil
Dalila
D
Souguir
Ezzedine
E
Ferjani
Olivier
O
Faure
Adnane
A
Hitmi
Gérard
G
Ledoigt
D001151:Arsenic / Q000633:toxicity*; D002857:Chromium / Q000633:toxicity*; D030561:Databases, Nucleic Acid; D018506:Gene Expression Regulation, Plant / Q000187:drug effects*; D018527:Hydroponics; D018551:Solanum lycopersicum / Q000187:drug effects* / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012689:Sequence Homology, Nucleic Acid; D012996:Solutions; D013312:Stress, Physiological / Q000187:drug effects* / Q000235:genetics*
D012333:RNA, Messenger; D012996:Solutions; D002857:Chromium; D001151:Arsenic