Up-regulation of chloroplastic antioxidant capacity is involved in alleviation of nickel toxicity of Zea mays L. by exogenous salicylic acid.
A pot experiment was carried out to investigate the effect of exogenous salicylic acid (SA) on the growth, photosynthesis, oxidative stress and responses of chloroplastic antioxidant defense system of maize (Zea mays L.) plants grown in a nickel (Ni)-contaminated soil. The results indicate that exogenous SA significantly decreased the reduction in dry weight, chlorophyll and beta-carotene contents, and net photosynthetic rate of the Ni-stressed maize, demonstrating an alleviating effect of SA on Ni toxicity of plants. Superoxide anion generation rate, H(2)O(2) and malondialdehyde (MDA) contents, and lipoxygenase (LOX, EC 1.13.11.12) activity significantly increased in the chloroplasts of maize exposed to Ni stress, revealing an oxidative damage occurred in maize chloroplasts, whereas, the values of these parameters were markedly lowered in the SA-treated plants under Ni stress. Application of SA significantly enhanced the activities of superoxide dismutase (SOD, EC 1.15.1.1), ascorbate peroxidase (APX, EC 1.11.1.11), monodehydroascorbate reductase (MDHAR, EC 1.6.5.4), dehydroascorbate reductase (DHAR, EC 1.8.5.1) and glutathione reductase (GR, EC 1.6.4.2), and the poll of reduced ascorbate and glutathione in chloroplasts of the Ni-stressed maize. Accordingly, the fact that SA up-regulates the capacity of antioxidant defense system in chloroplasts, thus reducing the oxidative damage, is involved in the SA-induced alleviation of Ni toxicity in maize.
School of Life Sciences, Hunan University of Science and Technology, Xiangtan, Hunan, China. haihuawxt@163.com
Haihua
H
Wang
Tao
T
Feng
Xixu
X
Peng
Mingli
M
Yan
Xinke
X
Tang
D000975:Antioxidants / Q000378:metabolism*; D002734:Chlorophyll / Q000378:metabolism; D002736:Chloroplasts / Q000187:drug effects* / Q000378:metabolism; D004798:Enzymes / Q000378:metabolism; D005978:Glutathione / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000378:metabolism; D008084:Lipoxygenase / Q000378:metabolism; D008315:Malondialdehyde / Q000378:metabolism; D009532:Nickel / Q000633:toxicity*; D018384:Oxidative Stress / Q000187:drug effects*; D010788:Photosynthesis / Q000187:drug effects; D020156:Salicylic Acid / Q000494:pharmacology*; D012989:Soil Pollutants / Q000633:toxicity*; D013481:Superoxides / Q000378:metabolism; D015854:Up-Regulation; D003313:Zea mays / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D019207:beta Carotene / Q000378:metabolism
D000975:Antioxidants; D004798:Enzymes; D012989:Soil Pollutants; D019207:beta Carotene; D013481:Superoxides; D002734:Chlorophyll; C029938:nickel sulfate; D008315:Malondialdehyde; D009532:Nickel; D006861:Hydrogen Peroxide; D008084:Lipoxygenase; D005978:Glutathione; D020156:Salicylic Acid