Identification of a splice variant of the metal-responsive transcription factor MTF-1 in common carp.
Transactivation of the expression of metallothionein genes involves the Metal-responsive Transcription Factor (MTF-1). We report here the identification of mtf-1.1a, the first known splice variant of mtf-1.1 mRNA, in common carp (Cyprinus carpio). The lack of a 103 nt internal segment results in a frame shift, causing the early termination of translation. mtf-1.1a mRNA encodes a protein consisting of the first 349 amino acids of MTF-1.1 plus an additional 64 amino acids, with no significant similarity to any of the proteins in the databases. The predicted MTF-1.1a protein carries the Zn-finger domain and the nuclear exporting and nuclear localization signals, and lacks the transcription activation domains. mtf-1.1a was detected in all tissues examined but the liver, with the highest level in the brain. Arsenic alters the levels of both mtf-1.1 and mtf-1.1a transcripts, in an isoform- and tissue-specific manner.
Ferencz
Agnes
A
Department of Biochemistry and Molecular Biology, Faculty of Science, University of Szeged, Szeged, Hungary.
Hermesz
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D000595:Amino Acid Sequence; D000818:Animals; D002104:Cadmium / Q000633:toxicity; D002347:Carps / Q000235:genetics* / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000302:isolation & purification; D014644:Genetic Variation; D008969:Molecular Sequence Data; D020033:Protein Isoforms / Q000235:genetics*; D014157:Transcription Factors / Q000235:genetics* / Q000302:isolation & purification; D000097809:Transcription Factor MTF-1
D002104:Cadmium; D004268:DNA-Binding Proteins; D020033:Protein Isoforms; D014157:Transcription Factors; D000097809:Transcription Factor MTF-1