Digital ion trap mass spectrometer for probing the structure of biological macromolecules by gas phase X-ray scattering.
Small-angle X-ray scattering is a technique for the characterization and structural analysis of a variety of materials including biological macromolecules and polymers. For the conformational analysis of proteins, the interaction between sample and X-rays is generally performed when the proteins are present in solution. Here a three-dimensional digital ion trap interfaced with a high intensity X-ray source is built to prove that X-ray scattering can be performed on ions isolated in gas-phase. Initial experiments on an unresolved ion population of multiply charged cytochrome C ions indicate that a small-angle X-ray scattering signal can be detected and that partial structural information can be extracted about the overall molecular structure of protein ions.
Michael Barber Centre for Mass Spectrometry, Manchester Interdisciplinary Biocentre, The University of Manchester, Manchester, UK.
Bryan J
BJ
McCullough
Andrew
A
Entwistle
Ikuo
I
Konishi
Steven
S
Buffey
S Samar
SS
Hasnain
Francesco L
FL
Brancia
J Günter
JG
Grossmann
Simon J
SJ
Gaskell
D001704:Biopolymers / Q000737:chemistry*; D045304:Cytochromes c / Q000737:chemistry; D004867:Equipment Design; D005740:Gases / Q000737:chemistry*; D013058:Mass Spectrometry / Q000295:instrumentation / Q000379:methods*; D010455:Peptides / Q000737:chemistry; D053838:Scattering, Small Angle; D014961:X-Ray Diffraction
D001704:Biopolymers; D005740:Gases; D010455:Peptides; D045304:Cytochromes c