Electrophoretic mobility shift assays for the analysis of DNA-protein interactions.
Electromobility shift assay is a simple, efficient, and rapid method for the study of specific DNA-protein interactions. It relies on the reduction in the electrophoretic mobility conferred to a DNA fragment by an interacting protein. The technique is suitable to qualitative, quantitative, and kinetic analyses. It can also be used to analyze conformational changes.
Oncology and Molecular Endocrinology Research Center, CHUL, Centre Hospitalier Universitaire de Québec and Laval University, 2705 Laurier Blvd, Québec, QC, Canada, G1V 4G2.
Manon
M
Gaudreault
Marie-Eve
ME
Gingras
Maryse
M
Lessard
Steeve
S
Leclerc
Sylvain L
SL
Guérin
D004247:DNA / Q000378:metabolism*; D015342:DNA Probes / Q000302:isolation & purification; D024202:Electrophoretic Mobility Shift Assay / Q000379:methods*; D007553:Isotope Labeling; D009687:Nuclear Proteins / Q000378:metabolism; D011485:Protein Binding / Q000187:drug effects; D011506:Proteins / Q000378:metabolism*; D012965:Sodium Chloride / Q000494:pharmacology
D015342:DNA Probes; D009687:Nuclear Proteins; D011506:Proteins; D012965:Sodium Chloride; D004247:DNA