Effect of ultrasounds on the electrochemical synthesis of polypyrrole, application to the adhesion and growth of biological cells.
In this study, a new way to synthesize polypyrrole films is presented. This original way consists in the electropolymerization of polypyrrole under high frequency ultrasonic irradiation on conductive fluorine-doped tin oxide surfaces. The polypyrrole films obtained are then compared, in terms of chemical structure and morphology, to polypyrrole films synthesized by standard electrochemical methodology. Next, these polymer films are tested as an alternative to biomaterials that are commonly used as cell culture substrates. Thus, the adhesion and growth of osteoblastics cells and microbial cells on polymer-modified surfaces are investigated by using qualitative observation and quantitative tests. These studies proved the non-toxicity of the polymer films for osteoblastic and microbial cells but also a different behaviour of osteoblastic cells and microbial cells with polypyrrole films.
Institut UTINAM, UMR-CNRS 6213, Université de Franche-Comté, 16 route de Gray, 25030 Besançon Cedex, France. boris.lakard@univ-fcomte.fr
B
B
Lakard
L
L
Ploux
K
K
Anselme
F
F
Lallemand
S
S
Lakard
M
M
Nardin
J Y
JY
Hihn
D001422:Bacterial Adhesion / Q000187:drug effects; D001672:Biocompatible Materials / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology*; D002448:Cell Adhesion / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D004563:Electrochemistry / Q000379:methods*; D004926:Escherichia coli / Q000166:cytology / Q000187:drug effects; D005461:Fluorine / Q000737:chemistry; D006801:Humans; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects; D010087:Oxides / Q000737:chemistry; D011108:Polymers / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology*; D011758:Pyrroles / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology*; D013057:Spectrum Analysis; D013499:Surface Properties; D014465:Ultrasonics
D001672:Biocompatible Materials; D010087:Oxides; D011108:Polymers; D011758:Pyrroles; D005461:Fluorine; C067635:polypyrrole