Antigenotoxic effects of Citrus aurentium L. fruit peel oil on mutagenicity of two alkylating agents and two metals in the Drosophila wing spot test.
Antigenotoxic effects of Citrus aurentium L. (Rutaceae) fruit peel oil (CPO) in combination with mutagenic metals and alkylating agents were studied using the wing spot test of D. melanogaster. The four reference mutagens, potassium dichromate (K2Cr2O7), cobalt chloride (CoCl2), ethylmethanesulfonate (EMS), and N-ethyl-N-nitrosourea (ENU) were clearly genotoxic. CPO alone at doses from 0.1 to 0.5% in Tween 80 was not mutagenic and did not enhance the mutagenic effect of the reference mutagens. However, antigenotoxic effects of CPO were clearly demonstrated in chronic cotreatments with mutagens and oil, by a significant decrease in wing spots induced by all four mutagens. The D. melanogaster wing spot test was found to be a suitable assay for detecting antigenotoxic effects in vivo.
Demir
Eşref
E
Department of Biology, Faculty of Arts and Sciences, Akdeniz University, Antalya, Turkey.
Kocaoğlu
Serap
S
Cetin
Huseyin
H
Kaya
Bülent
B
D000477:Alkylating Agents / Q000633:toxicity*; D000818:Animals; D016587:Antimutagenic Agents / Q000494:pharmacology*; D002957:Citrus / Q000737:chemistry*; D003035:Cobalt / Q000633:toxicity; D004331:Drosophila melanogaster / Q000187:drug effects*; D005020:Ethyl Methanesulfonate / Q000633:toxicity; D005038:Ethylnitrosourea / Q000633:toxicity; D008670:Metals / Q000633:toxicity*; D009152:Mutagenicity Tests; D009154:Mutation / Q000235:genetics; D010938:Plant Oils / Q000737:chemistry / Q000494:pharmacology*; D011192:Potassium Dichromate / Q000633:toxicity; D014921:Wings, Animal / Q000187:drug effects*
D000477:Alkylating Agents; D016587:Antimutagenic Agents; D008670:Metals; D010938:Plant Oils; D003035:Cobalt; D005020:Ethyl Methanesulfonate; C018021:cobaltous chloride; D005038:Ethylnitrosourea; D011192:Potassium Dichromate