HuR involvement in mitotic clonal expansion during acquisition of the adipocyte phenotype.
In the nucleus HuR binds to mRNAs containing adenylate-uridylate rich elements in the 3'-untranslated region. HuR may influence expression of its ligand mRNA through regulation of polyadenylation, translocation of the message to the cytosol, stabilization of the mRNA and/or altering its translational efficiency. Suppression of HuR using siRNA resulted in an attenuation of the 3T3-L1 differentiation program, consistent with HuR control of the expression of mRNA ligand(s) critical to the differentiation process. In the present study, we begin to identify mRNA ligands of HuR whose regulated expression is necessary for adipogenesis.
Karschner
Vesna A
VA
Department of Biochemistry and Molecular Biology, The Brody School of Medicine at East Carolina University, Greenville, NC 27858, USA.
Pekala
Phillip H
PH
D041721:3T3-L1 Cells; D017667:Adipocytes / Q000166:cytology / Q000378:metabolism / Q000502:physiology*; D050156:Adipogenesis / Q000235:genetics*; D000818:Animals; D000954:Antigens, Surface / Q000235:genetics / Q000378:metabolism*; D002999:Clone Cells; D051959:ELAV Proteins; D000067780:ELAV-Like Protein 1; D008024:Ligands; D051379:Mice; D008938:Mitosis; D010641:Phenotype; D012333:RNA, Messenger / Q000378:metabolism*; D034741:RNA, Small Interfering / Q000235:genetics; D016601:RNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*
D000954:Antigens, Surface; D051959:ELAV Proteins; D000067780:ELAV-Like Protein 1; C098770:ELAVL1 protein, human; D008024:Ligands; D012333:RNA, Messenger; D034741:RNA, Small Interfering; D016601:RNA-Binding Proteins