Distinct spatiotemporal pattern of CNS lesions revealed by USPIO-enhanced MRI in MOG-induced EAE rats implicates the involvement of spino-olivocerebellar pathways.
USPIO-enhanced MRI allows non-invasive visualization of mononuclear cell infiltration into CNS lesions in MS and EAE. Herein, we show a distinct spatiotemporal pattern of CNS lesions that reveals the involvement of spino-olivocerebellar pathways in MOG-induced EAE rats using USPIO-enhanced MRI. Specifically, lesions of the inferior olives were observed primarily in the acute phase whereas lesions of cerebellum or spinal cord/brainstem were observed during the relapse phase. Further, behavioral deficits observed from these animals are consistent with the functional role of spino-olivocerebellar pathways in coordination and movement. Collectively, our results provide new insights into the pathophysiology of this animal model of MS.
Chin
Chih-Liang
CL
Advanced Technology, Global Pharmaceutical Research and Development, Abbott Laboratories, Abbott Park, IL 60064, USA. chih-liang.chin@abbott.com
Pai
Madhavi
M
Bousquet
Peter F
PF
Schwartz
Annette J
AJ
O'Connor
Elizabeth M
EM
Nelson
Christine M
CM
Hradil
Vincent P
VP
Cox
Bryan F
BF
McRae
Bradford L
BL
Fox
Gerard B
GB
D000818:Animals; D001921:Brain / Q000473:pathology*; D002531:Cerebellum / Q000473:pathology; D003911:Dextrans; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000473:pathology*; D005260:Female; D052203:Ferrosoferric Oxide; D007150:Immunohistochemistry; D007501:Iron; D008279:Magnetic Resonance Imaging / Q000379:methods; D058185:Magnetite Nanoparticles; D009043:Motor Activity / Q000502:physiology; D009185:Myelin Proteins; D018992:Myelin-Associated Glycoprotein / Q000276:immunology / Q000633:toxicity; D063308:Myelin-Oligodendrocyte Glycoprotein; D009434:Neural Pathways / Q000473:pathology*; D009847:Olivary Nucleus / Q000473:pathology; D010087:Oxides; D019275:Radiopharmaceuticals; D051381:Rats; D013116:Spinal Cord / Q000473:pathology*
D003911:Dextrans; D058185:Magnetite Nanoparticles; C571328:Mog protein, rat; D009185:Myelin Proteins; D018992:Myelin-Associated Glycoprotein; D063308:Myelin-Oligodendrocyte Glycoprotein; D010087:Oxides; D019275:Radiopharmaceuticals; C097921:ferumoxtran-10; D007501:Iron; D052203:Ferrosoferric Oxide