Disulfide-crosslinked electrospun poly(gamma-glutamic acid) nonwovens as reduction-responsive scaffolds.
Novel water-insoluble, and reduction-responsive nonwoven scaffolds were fabricated from gamma-PGA and tested in cell culture. An electrospinning method was developed to produce scaffolds of fibers with diameters of 0.05-0.5 microm. Crosslinking of the fibers with cystamine in the presence of EDC resulted in water-insoluble gamma-PGA nonwovens with disulfide crosslinkages. These crosslinked fibers were easily decomposed under physiological conditions using L-cysteine, a biocompatible reductant. In vitro experiments with mouse L929 fibroblasts showed good adhesion onto gamma-PGA-SS fiber matrices and excellent cell proliferation. These gamma-PGA-SS nonwovens can be used as novel biocompatible and biodegradable scaffolds with reduction-responsiveness for biomedical or tissue engineering applications.
Department of Applied Chemistry, Graduate School of Engineering, Osaka University, 2-1 Yamada-oka, Suita 565-0871, Japan.
Hiroaki
H
Yoshida
Kristina
K
Klinkhammer
Michiya
M
Matsusaki
Martin
M
Möller
Doris
D
Klee
Mitsuru
M
Akashi
D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects; D002452:Cell Count; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D003432:Cross-Linking Reagents / Q000737:chemistry* / Q000494:pharmacology; D003545:Cysteine; D004220:Disulfides / Q000737:chemistry*; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects / Q000648:ultrastructure; D051379:Mice; D011099:Polyglutamic Acid / Q000031:analogs & derivatives* / Q000737:chemistry / Q000494:pharmacology; D013997:Time Factors; D054457:Tissue Scaffolds
D003432:Cross-Linking Reagents; D004220:Disulfides; C511775:poly(gamma-glutamic acid); D011099:Polyglutamic Acid; D003545:Cysteine