Fluorescence resonance energy transfer between an anionic conjugated polymer and a dye-labeled lysozyme aptamer for specific lysozyme detection.
Surface charge switch of a 6-carboxyfluorescein (FAM) labeled lysozyme aptamer from negative to positive upon lysozyme binding triggers fluorescence resonance energy transfer (FRET) between an anionic conjugated polymer and the aptamer, which provides a convenient method for lysozyme detection in biological media with high sensitivity and selectivity.
Wang
Jing
J
Department of Chemical & Biomolecular Engineering, National University of Singapore, 4 Engineering Drive 4, Singapore.
Liu
Bin
B
D000838:Anions / Q000737:chemistry; D052158:Aptamers, Peptide / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods*; D005456:Fluorescent Dyes / Q000032:analysis* / Q000737:chemistry*; D015394:Molecular Structure; D009113:Muramidase / Q000032:analysis* / Q000737:chemistry* / Q000378:metabolism; D011108:Polymers / Q000737:chemistry*; D012680:Sensitivity and Specificity; D013379:Substrate Specificity
D000838:Anions; D052158:Aptamers, Peptide; D005456:Fluorescent Dyes; D011108:Polymers; D009113:Muramidase