The generation of highly purified primary human neutrophils and assessment of apoptosis in response to Toll-like receptor ligands.
Neutrophils are crucial components of our defence against microbial assault. They are short-lived cells, with regulation of their lifespan being a primary mechanism involved in the regulation of their function. Delay of apoptosis facilitates their clearance of pathogens, whilst appropriate induction of cell death facilitates wound healing. A variety of methods are available to study neutrophil function: purification of human neutrophils and analysis of their lifespan are described here.
Parker
Lisa C
LC
School of Medicine and Biomedical Sciences, University of Sheffield, Sheffield S10 2JF, UK.
Prince
Lynne R
LR
Buttle
David J
DJ
Sabroe
Ian
I
D017209:Apoptosis; D053148:Caspase 3 / Q000378:metabolism; D002469:Cell Separation / Q000379:methods*; D002478:Cells, Cultured; D006801:Humans; D008024:Ligands; D009504:Neutrophils / Q000166:cytology* / Q000378:metabolism*; D051193:Toll-Like Receptors / Q000378:metabolism*
D008024:Ligands; D051193:Toll-Like Receptors; D053148:Caspase 3