Structure and dynamics of the influenza A M2 channel: a comparison of three structures.
The M2 protein is an essential component of the Influenza virus' infectivity cycle. It is a homo-tetrameric bundle forming a pH-gated H(+) channel. The structure of M2 was solved by three different groups, using different techniques, protein sequences and pH environment. For example, solid-state NMR spectroscopy was used on a protein in lipid bilayers, while X-ray crystallography and solution NMR spectroscopy were applied on a protein in detergent micelles. The resulting structures from the above efforts are rather distinct. Herein, we examine the different structures under uniform conditions such as a lipid bilayer and specified protonation state. We employ extensive molecular dynamics simulations, in several protonation states, representing both closed and open forms of the channel. Exploring the properties of each of these structures has shown that the X-ray structure is more stable than the other structures according to various criteria, although its water conductance and water-wire formation do not correlate to the protonation state of the channel.
Department of Biological Chemistry, The Alexander Silberman Institute of Life Sciences, The Hebrew University of Jerusalem, Edmund J. Safra Campus Givat-Ram, Jerusalem, Israel.
Hadas
H
Leonov
Isaiah T
IT
Arkin
D003198:Computer Simulation; D006639:Histidine / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy; D008958:Models, Molecular; D016062:Porosity; D055550:Protein Stability; D017433:Protein Structure, Secondary; D011522:Protons; D012996:Solutions; D014763:Viral Matrix Proteins / Q000737:chemistry*; D014867:Water / Q000737:chemistry; D000086082:Viroporin Proteins
D006639:Histidine; D011522:Protons; D012996:Solutions; D014763:Viral Matrix Proteins; D014867:Water; C488937:M2 protein, Influenza A virus; D000086082:Viroporin Proteins