Acinetobacter antiviralis sp. nov., from Tobacco plant roots.
Acinetobacter strain KNF2022T was isolated from tobacco plant roots during the screening of antiviral substances having inhibitory effects on Tobacco mosaic virus (TMV) and examined by phenotypic, chemotaxonomic, and genetic characterization. It was a nonmotile, Gram-negative bacterium. This strain contained Q-9 as the main respiratory quinone. The major cellular fatty acids of the isolate were 16:0, 18:1 w9c, and 16:1 w7c/15 iso 2OH. The DNA base composition was 44 mol%. Phylogenetic analysis based on the 16S rRNA sequence revealed that the isolate formed an evolutionary lineage distinct from other Acinetobacter species. Based on the evaluation of morphologic, physiologic, and chemotaxonomic characteristics, DNA-DNA hybridization values, and 16S rRNA sequence comparison, we propose the new species Acinetobacter antiviralis sp. nov., the type strain of which is KNF2022T(=KCTC0699BPT).
Lee
Jung-Sook
JS
Korean Collection for Type Cultures, Biological Resource Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon 305-806, Korea. jslee@kribb.re.kr
Lee
Keun Chul
KC
Kim
Kwang Kyu
KK
Hwang
In Cheon
IC
Jang
Cheol
C
Kim
Nam Gyu
NG
Yeo
Woon Hyung
WH
Kim
Beom Seok
BS
Yu
Yong Man
YM
Ahn
Jong Seog
JS
D000150:Acinetobacter / Q000145:classification* / Q000302:isolation & purification* / Q000502:physiology; D004269:DNA, Bacterial / Q000302:isolation & purification; D005227:Fatty Acids / Q000378:metabolism; D008969:Molecular Sequence Data; D010802:Phylogeny; D018517:Plant Roots / Q000382:microbiology*; D011809:Quinones / Q000378:metabolism; D012336:RNA, Ribosomal, 16S / Q000032:analysis / Q000235:genetics; D017422:Sequence Analysis, DNA; D014026:Nicotiana / Q000382:microbiology*; D014027:Tobacco Mosaic Virus / Q000254:growth & development
D004269:DNA, Bacterial; D005227:Fatty Acids; D011809:Quinones; D012336:RNA, Ribosomal, 16S