A soft and flexible biosensor using a phospholipid polymer for continuous glucose monitoring.
A flexible biosensor using a phospholipid polymer to immobilization of glucose oxidase (GOD) was fabricated and tested. At first, an enzyme membrane formed by immobilizing GOD onto a porous polytetrafluoroethylene (PTFE) membrane using the phospholipid polymer (2-methacryloyloxyethyl phosphorylcholine (MPC) copolymerized with 2-ethylhexylmethacrylate (EHMA):PMEH) was evaluated. According to the result of amperometric measurement, average density of GOD to be immobilized was optimized to 38.9 units cm(-2). Temperature and pH dependences were also investigated. Then, a flexible glucose sensor was fabricated by immobilizing GOD onto a flexible hydrogen peroxide electrode using PMEH. The flexible glucose sensor showed a linear relationship between output currents and glucose concentration in 0.05-1.00 mmol L(-1), with a correlation coefficient of 0.999. The calibration range covered the normal tear glucose level of 0.14-0.23 mmol L(-1). This indicates that the flexible biosensor is considered to be useful for monitoring of glucose in tear fluids.
Chu
MingXing
M
Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, 2-3-10 Kanda-Surugadai, Chiyoda-ku, Tokyo 101-0062, Japan.
Kudo
Hiroyuki
H
Shirai
Takayuki
T
Miyajima
Kumiko
K
Saito
Hirokazu
H
Morimoto
Nobuyuki
N
Yano
Kazuyoshi
K
Iwasaki
Yasuhiko
Y
Akiyoshi
Kazunari
K
Mitsubayashi
Kohji
K
D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods*; D004800:Enzymes, Immobilized / Q000737:chemistry; D005947:Glucose / Q000032:analysis*; D005949:Glucose Oxidase / Q000737:chemistry; D006801:Humans; D008567:Membranes, Artificial; D008689:Methacrylates / Q000737:chemistry*; D010767:Phosphorylcholine / Q000031:analogs & derivatives* / Q000737:chemistry; D018583:Pliability; D011138:Polytetrafluoroethylene / Q000737:chemistry*; D012680:Sensitivity and Specificity
D004800:Enzymes, Immobilized; D008567:Membranes, Artificial; D008689:Methacrylates; D010767:Phosphorylcholine; C070638:2-methacryloyloxyethyl phosphorylcholine; D011138:Polytetrafluoroethylene; D005949:Glucose Oxidase; D005947:Glucose