[Obtaining and characteristics of domestic preparation interferon alpha-2b with prolonged effect].
Pegylated interferon alpha-2b (PEG-IFN alpha-2b) is a domestic preparation of a modified recombinant interferon alpha-2b with prolonged effect. The preparation was obtained by N-terminal pegylation of IFN alpha-2b with polyethylene glycol (PEG). This paper presents the method of PEG-IFN alpha-2b synthesis and characteristics of the obtained product. PAAG electrophoresis, Western blot analysis and MALDI-TOF mass-spectrometry confirm that the preparation is an N-terminal pegylated IFN alpha-2b that contains no more than 10% of dipegylated IFN alpha-2b. The comparison of PEG-IFN alpha-2b with its foreign analogue has revealed the similarity of their biological activity and pharmacokinetic parameters.
Pokholenko
Ia A
IaA
Porubleva
L V
LV
Dubeĭ
I Ia
IIa
Rebriev
A V
AV
Sutugina
L P
LP
Gromovoĭ
T Iu
TIu
Pokrovskiĭ
V A
VA
Obolenskaia
M Iu
MIu
Chernykh
S I
SI
D000818:Animals; D000998:Antiviral Agents / Q000008:administration & dosage / Q000097:blood / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D002417:Cattle; D002460:Cell Line; D003588:Cytopathogenic Effect, Viral / Q000187:drug effects; D003692:Delayed-Action Preparations; D004337:Drug Carriers / Q000737:chemistry; D006801:Humans; D000077190:Interferon alpha-2; D016898:Interferon-alpha / Q000008:administration & dosage / Q000097:blood / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D008958:Models, Molecular; D011092:Polyethylene Glycols; D051381:Rats; D017208:Rats, Wistar; D011994:Recombinant Proteins; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013678:Technology, Pharmaceutical / Q000379:methods*
D000998:Antiviral Agents; D003692:Delayed-Action Preparations; D004337:Drug Carriers; D000077190:Interferon alpha-2; D016898:Interferon-alpha; D011994:Recombinant Proteins; D011092:Polyethylene Glycols; C417083:peginterferon alfa-2b