Down-regulation of miR-141 in gastric cancer and its involvement in cell growth.
OBJECTIVE
Human microRNA-141 (miR-141), a member of the miR-200 family, has been reported to be associated with various human malignancies. However, it remains unknown whether miR-141 is involved in the pathogenesis of gastric cancer. Therefore, we examined the expression of miR-141 in gastric cancer tissues and the effect of miR-141 overexpression on cancer cell proliferation.


METHODS
The expression level of miR-141 in 35 pair-matched gastric neoplastic and adjacent non-neoplastic tissues, and in 5 gastric cancer cell lines were examined by quantitative real-time PCR. The growth of MGC-803 cells transfected with miRNA precursor was examined by MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl-tetrazoliumbromide) assay.


RESULTS
MiR-141 was significantly down-regulated in 80% (28/35) of primary gastric cancer tissues compared with pair-matched adjacent non-tumor tissues (P < 0.01). The expression of miR-141 was also found to be substantially reduced in several human gastric cancer cell lines such as MGC-803, HGC-27, SGC-7901 and BGC-823 cells. Overexpression of miR-141 with its precursors significantly inhibited the proliferation of gastric cancer cells.


CONCLUSIONS
These results suggest that miR-141 may be involved in the development of gastric cancer through its inhibitory effect on cell proliferation.
Du
Ying
Y
Gastroenterology Laboratory, Clinical Research Institute, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, 310016 Hangzhou, People's Republic of China.
Xu
Yanjun
Y
Ding
Ling
L
Yao
Haomi
H
Yu
Hong
H
Zhou
Tianhua
T
Si
Jianmin
J
D000368:Aged; D000369:Aged, 80 and over; D049109:Cell Proliferation; D015536:Down-Regulation / Q000502:physiology; D005260:Female; D015972:Gene Expression Regulation, Neoplastic / Q000502:physiology; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000378:metabolism / Q000502:physiology*; D008875:Middle Aged; D013274:Stomach Neoplasms / Q000235:genetics / Q000473:pathology / Q000503:physiopathology*; D014407:Tumor Cells, Cultured
C533895:MIRN141 microRNA, human; D035683:MicroRNAs