[The expression of Toll-like receptor 8 and cytokines in rheumatoid arthritis mice induced by chicken II collogen].
OBJECTIVE
To explore the expression of Toll-like receptor 8(TLR8)in rheumatoid arthritis induced by chicken II collogen in mice and analyze the relation of TLR8 to IL-10, IL-17A and IL-1beta.


METHODS
Twelve DBA/1J mice were randomly divided into model group(T) and negative group(NC). The paw swelling was seen at the day of 27 post the first immunization with chicken II collogen. The serum TNF-alpha was detected by ELISA and inflammatory cell infiltration was examined by HE. Real-time PCR was used to analysis the expression of IL-10, IL-17A, IL-1beta and TLR8 in spleen mononuclear cells.


RESULTS
Severe inflammation was detected in model group mice by histological analysis, which was not seen in negative group. The serum TNF-alpha in model group was enhanced compared with that in negative group(P<0.01). The expression levels of IL-10, IL-17A, TLR8 and IL-1beta in spleen mononuclear cells from model group were increased compared with those from negative group(P<0.01, P<0.05). Statistical analysis showed that there was negative correlation between the expression of TLR8 and IL-10(P<0.01), and there was no correlation between the expression of TLR8 and IL-17A or IL-1beta(P>0.05).


CONCLUSIONS
The expression of TLR8 was increased in rheumatoid arthritis-mice induced by chicken II collogen, and there was negative correlation between the expression of TLR8 and the expression of IL-10.
Mao
Fei
F
School of Medical Science and Laboratory Medicine, Jiangsu University, Zhenjiang 212013, China. maofei2003@ujs.edu.cn
Xu
Wen-rong
WR
Qian
Hui
H
Zhu
Wei
W
Yan
Yong-min
YM
Gao
Shuo
S
Xu
Hua-xi
HX
D000818:Animals; D001169:Arthritis, Experimental / Q000097:blood / Q000235:genetics / Q000473:pathology; D001172:Arthritis, Rheumatoid / Q000097:blood / Q000139:chemically induced / Q000235:genetics*; D002645:Chickens; D024043:Collagen Type II; D016207:Cytokines / Q000097:blood / Q000235:genetics*; D004797:Enzyme-Linked Immunosorbent Assay; D020869:Gene Expression Profiling; D016753:Interleukin-10 / Q000235:genetics; D020381:Interleukin-17 / Q000235:genetics; D053583:Interleukin-1beta / Q000235:genetics; D007963:Leukocytes, Mononuclear / Q000378:metabolism / Q000473:pathology; D051379:Mice; D008811:Mice, Inbred DBA; D011897:Random Allocation; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013154:Spleen / Q000378:metabolism / Q000473:pathology; D051216:Toll-Like Receptor 6 / Q000235:genetics*; D014409:Tumor Necrosis Factor-alpha / Q000097:blood / Q000235:genetics
D024043:Collagen Type II; D016207:Cytokines; D020381:Interleukin-17; D053583:Interleukin-1beta; C495369:Tlr6 protein, mouse; D051216:Toll-Like Receptor 6; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10