Survival of Vibrio parahaemolyticus under environmental stresses as influenced by growth phase and pre-adaptation treatment.
In this study, the susceptibility of Vibrio parahaemolyticus in different growth phases after exposure to lethal stresses including 47 degrees C and 8% ethanol was first investigated. The effect of a culture's growth phase on both the heat and ethanol shock response of V. parahaemolyticus was then examined. It was found that cells of V. parahaemolyticus in the mid-exponential phase, regardless of adaptation, were most susceptible to environmental stresses, while cells in the stationary phase were least susceptible to the lethal stresses examined. Adaptation with heat shock at 42 degrees C for 45 min or ethanol shock with 5% ethanol for 60 min induced an increased resistance of V. parahaemolyticus to subsequent lethal stresses at 47 degrees C and 8% ethanol. While the adaptation treatments resulted in a reduced resistance of the test organism to pH 4.4 and 20% NaCl. Generally, the extent of changes in the resistance of V. parahaemolyticus to lethal stresses between the adapted and control cells was found to be growth phase dependent. Compared with the respective control cells, the adapted late-exponential phase cells exhibited the greatest extent of change, while the adapted stationary phase cells showed the least change in their resistance to the lethal stresses examined.
Graduate Institute of Food Science and Technology, National Taiwan University, 59 Lane 144, Keelung Rd., Sec. 4, Taipei, Taiwan.
Ming-Lun
ML
Chiang
Cheng-Chun
CC
Chou
D000222:Adaptation, Physiological; D015169:Colony Count, Microbial; D004305:Dose-Response Relationship, Drug; D000431:Ethanol / Q000633:toxicity*; D005506:Food Contamination / Q000032:analysis / Q000517:prevention & control; D005516:Food Microbiology; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D050296:Microbial Viability / Q000187:drug effects; D008954:Models, Biological; D012965:Sodium Chloride / Q000494:pharmacology; D013312:Stress, Physiological; D014736:Vibrio parahaemolyticus / Q000187:drug effects / Q000254:growth & development / Q000502:physiology*
D000431:Ethanol; D012965:Sodium Chloride