Colorimetric sensing by using allosteric-DNAzyme-coupled rolling circle amplification and a peptide nucleic acid-organic dye probe.
Target detection by the naked eye: The action of an RNA-cleaving allosteric DNAzyme in response to ligand binding was coupled to a rolling circle amplification process to generate long single-stranded DNA molecules for colorimetric sensing (see scheme). Upon hybridization of the resulting DNA with a complementary PNA sequence in the presence of a duplex-binding dye, the color of the dye changed from blue to purple.
Department of Biochemistry and Biomedical Sciences, McMaster University, 1200 Main Street West, Hamilton, ON L8N 3Z5, Canada.
M Monsur
MM
Ali
Yingfu
Y
Li
D000494:Allosteric Regulation; D015374:Biosensing Techniques; D003124:Colorimetry / Q000379:methods*; D004396:Coloring Agents / Q000378:metabolism*; D004261:DNA Replication; D021881:DNA, Catalytic / Q000737:chemistry*; D004277:DNA, Single-Stranded / Q000096:biosynthesis* / Q000737:chemistry; D009693:Nucleic Acid Hybridization; D020135:Peptide Nucleic Acids / Q000737:chemistry*
D004396:Coloring Agents; D021881:DNA, Catalytic; D004277:DNA, Single-Stranded; D020135:Peptide Nucleic Acids