A preclinical assessment of the safety and biodistribution of an adenoviral vector containing the herpes simplex virus thymidine kinase gene (Cerepro) after intracerebral administration.
BACKGROUND
Cerepro (sitimagene ceradenovec) is an adenoviral vector containing herpes simplex virus thymidine kinase gene (HSV-tk), which is being developed for the treatment of high-grade glioma with oral ganciclovir (GCV). The nonclinical safety and biodistribution of Cerepro were assessed following intravenous (i.v.) or intracerebral (i.c.) injection.


METHODS
Crl : WI(GLX/BRL/Han) rats (n = 198) were injected i.c. or i.v. with Cerepro or vehicle control, with GCV by intraperitoneal (i.p.) injection to selected groups. Safety was assessed by observation of animal behaviour and post mortem histology. Antibody response was assessed, and biodistribution measured using the quantitative polymerase chain reaction (PCR) and reverse transcriptase-PCR in blood and tissues.


RESULTS
Following i.v. or i.c. injection, there was no antibody response and no effect on behaviour, body weight, food consumption or haematological and clinical chemistry parameters. Minor needle track changes were observed in control and Cerepro-i.c. injection groups. Transient myeloid hyperplasia was observed in five of the 24 animals in the i.v. injection group and spleen weight increased in both the i.c. and i.v. groups. Cerepro was detected in the brain and at low levels in blood and spleen following i.c. injection, decreasing with time. Following i.v. injection, Cerepro was detected in viscera and blood, decreasing with time. Transcription of Cerepro was detected in the brain following i.c. injection, with lower levels in spleen; following i.v. injection, transcription was seen in viscera. Germline integration was not seen.


CONCLUSIONS
Intracerebral injection of Cerepro is safe and produces a high level of transgene expression in the brain, with limited biodistribution.
Langford
Gillian
G
Ark Therapeutics Ltd, London, UK.
Dayan
Anthony
A
Yla-Herttuala
Seppo
S
Eckland
David
D
D000818:Animals; D005260:Female; D015774:Ganciclovir / Q000627:therapeutic use; D015316:Genetic Therapy; D005822:Genetic Vectors / Q000008:administration & dosage / Q000493:pharmacokinetics* / Q000633:toxicity*; D007150:Immunohistochemistry; D008297:Male; D051381:Rats; D018139:Simplexvirus / Q000235:genetics*; D013937:Thymidine Kinase / Q000008:administration & dosage / Q000235:genetics / Q000493:pharmacokinetics* / Q000633:toxicity*
D013937:Thymidine Kinase; C529697:sitimagene ceradenovec; D015774:Ganciclovir