RP-HPLC determination of phenylalkanoids and monoterpenoids in Rhodiola rosea and identification by LC-ESI-TOF.
An HPLC method permitting the simultaneous determination of fourteen analytes (phenylalkanoids and monoterpenoids) from the roots of Rhodiola rosea was developed. A separation was achieved within 35 min using C(18) column material and a water-acetonitrile mobile phase, both containing a 0.05% phosphoric acid gradient system and a temperature of 53 degrees C. The method was validated for linearity, repeatability, limits of detection and limits of quantification. The limits of detection and limits of quantification of 14 phenylalkanoids and monoterpenoids were found to be 0.20-1.0 and 0.5-3.5 microg/mL, respectively. The wavelengths used for quantification of phenylalkanoids and monoterpenoids with a diode array detector were 205, 220 and 251 nm. The method was used to analyze the roots of two species of Rhodiola and commercial extracts of R. rosea and provides preliminary evidence of phytochemical differences between North American and Eurasian populations of R. rosea. LC-mass spectrometry coupled with electrospray ionization (ESI) interface method is described for the identification of phenylalkanoids and monoterpenoids in various Rhodiola samples. This method involved the use of the [M + H](+), [M + NH(4)](+) and [M + Na](+) ions in the positive ion mode with extractive ion monitoring (EIM).
National Center for Natural Products Research, Research Institute of Pharmaceutical Sciences, The University of Mississippi, MS 38677, USA.
Bharathi
B
Avula
Yan-Hong
YH
Wang
Zulfiqar
Z
Ali
Troy J
TJ
Smillie
Vicky
V
Filion
Alain
A
Cuerrier
John T
JT
Arnason
Ikhlas A
IA
Khan
D001555:Benzene Derivatives / Q000032:analysis* / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004187:Disaccharides / Q000032:analysis* / Q000302:isolation & purification; D016014:Linear Models; D039821:Monoterpenes / Q000032:analysis* / Q000302:isolation & purification; D010936:Plant Extracts / Q000032:analysis / Q000302:isolation & purification; D018517:Plant Roots / Q000737:chemistry*; D015203:Reproducibility of Results; D029771:Rhodiola / Q000737:chemistry*; D012680:Sensitivity and Specificity; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
D001555:Benzene Derivatives; D004187:Disaccharides; D039821:Monoterpenes; D010936:Plant Extracts