Diphenyl diselenide decreases serum levels of total cholesterol and tissue oxidative stress in cholesterol-fed rabbits.
Hypercholesterolaemia and oxidative stress are well-known risk factors in coronary artery diseases. Diphenyl diselenide is a synthetic organoselenium compound that has been shown to have in vitro and in vivo antioxidant properties. In this study, we investigated whether diphenyl diselenide could reduce the hypercholesterolaemia and diminish the tissue oxidative stress in cholesterol-fed rabbits. Twenty-four New Zealand white male rabbits were randomly divided into four groups. Each group was fed a different diet as follows: Control group--regular chow; Cholesterol group--1% cholesterol-enriched diet; diphenyl diselenide group--regular diet supplemented with 10 ppm diphenyl diselenide; and Chol/diphenyl diselenide group--the same cholesterol-rich supplemented with 10 ppm diphenyl diselenide. After 45 days of treatment, the rabbits were killed and the blood, liver, and brain were used for laboratory analysis. The results showed that the serum levels of total cholesterol were markedly increased in cholesterol-fed rabbits and the consumption of diphenyl diselenide decreased these levels approximately twofold in Chol/diphenyl diselenide rabbits (P < 0.05). The intake of diphenyl diselenide by hypercholesterolaemic rabbits diminished the serum and hepatic thiobarbituric acid reactive substances levels as well as the production of reactive oxygen species in the blood and brain (P < 0.05) when compared to the cholesterol group. In addition, diphenyl diselenide supplementation increased hepatic and cerebral delta-aminolevulinic dehydratase activity and hepatic non-protein thiol groups levels despite hypercholesterolaemia (P < 0.05). In summary, the results showed that diphenyl diselenide reduced the hypercholesterolaemia and the oxidative stress in cholesterol-fed rabbits.
Department of Biochemistry, Federal University of Santa Catarina, Florianópolis, SC, Brazil. andrezadebem@ccb.ufsc.br
Andreza Fabro
AF
de Bem
Rafael de Lima
Rde L
Portella
Elisângela
E
Colpo
Marta Maria Medeiros Frescura
MM
Duarte
Andressa
A
Frediane
Paulo Sergio
PS
Taube
Cristina Wayne
CW
Nogueira
Marcelo
M
Farina
Edson Luiz
EL
da Silva
João Batista
JB
Teixeira Rocha
D000821:Animal Feed; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001205:Ascorbic Acid / Q000032:analysis; D001555:Benzene Derivatives / Q000494:pharmacology*; D001921:Brain / Q000187:drug effects / Q000201:enzymology; D001923:Brain Chemistry / Q000187:drug effects; D002784:Cholesterol / Q000097:blood*; D002791:Cholesterol, Dietary / Q000008:administration & dosage*; D006937:Hypercholesterolemia / Q000139:chemically induced; D008099:Liver / Q000737:chemistry / Q000187:drug effects / Q000201:enzymology; D008297:Male; D016566:Organoselenium Compounds / Q000494:pharmacology*; D018384:Oxidative Stress / Q000187:drug effects*; D000623:Porphobilinogen Synthase / Q000097:blood*; D011817:Rabbits; D011897:Random Allocation; D017382:Reactive Oxygen Species / Q000097:blood / Q000378:metabolism*; D017392:Thiobarbituric Acid Reactive Substances / Q000032:analysis; D014280:Triglycerides / Q000097:blood
D000975:Antioxidants; D001555:Benzene Derivatives; D002791:Cholesterol, Dietary; D016566:Organoselenium Compounds; D017382:Reactive Oxygen Species; D017392:Thiobarbituric Acid Reactive Substances; D014280:Triglycerides; C061132:diphenyldiselenide; D002784:Cholesterol; D000623:Porphobilinogen Synthase; D001205:Ascorbic Acid