Activation of the brain-specific neurogranin gene in murine T-cell lymphomas by proviral insertional mutagenesis.
Neurogranin (Nrgn) is a highly expressed brain-specific protein, which sequesters calmodulin at low Ca(2+)-levels. We report here on retroviral activation of the Nrgn gene in tumors induced by the T-cell lymphomagenic SL3-3 murine leukemia virus. We have performed a systematic expression analysis of Nrgn in various mouse tissues and SL3-3 induced T-cell tumors. This demonstrated that insertional activation of Nrgn increased RNA and protein expression levels to that observed in brain. Furthermore, elevated Nrgn expression was also observed in some T-cell tumors with no detected provirus integrations into this genomic region. The presented data demonstrate that Nrgn can be produced at high levels outside the brain, and suggest a novel oncogenic role in T-cell lymphomas in mice.
Department of Molecular Biology, Aarhus University, Denmark.
Anne Ahlmann
AA
Nielsen
Kristín Rós
KR
Kjartansdóttir
Mads Heilskov
MH
Rasmussen
Annette Balle
AB
Sørensen
Bruce
B
Wang
Matthias
M
Wabl
Finn Skou
FS
Pedersen
D000818:Animals; D001921:Brain / Q000378:metabolism*; D015973:Gene Expression Regulation, Leukemic; D009052:Leukemia Virus, Murine / Q000235:genetics*; D016399:Lymphoma, T-Cell / Q000235:genetics* / Q000821:virology; D051379:Mice; D016254:Mutagenesis, Insertional; D051579:Neurogranin / Q000235:genetics*; D011533:Proviruses / Q000235:genetics; D015533:Transcriptional Activation
C496650:Nrgn protein, mouse; D051579:Neurogranin