Two-dimensional liquid chromatography analysis of synthetic polymers using fast size exclusion chromatography at high column temperature.
In recent years, two-dimensional liquid chromatography (2D-LC) has been used increasingly for the analysis of synthetic polymers. A 2D-LC analysis provides richer information than a single chromatography analysis at the cost of longer analysis time. The time required for a comprehensive 2D-LC analysis is essentially proportional to the analysis time of the second dimension separation. Many of 2D-LC analyses of synthetic polymers have employed size exclusion chromatography (SEC) for the second-dimension analysis due to the relatively short analysis time in addition to the wide use in the polymer analysis. Nonetheless, short SEC columns are often used for 2D-LC analyses to reduce the separation time, which inevitably deteriorates the resolution. In this study, we demonstrated that high temperature SEC can be employed as an efficient second-LC in the 2D-LC separation of synthetic polymers. By virtue of high temperature operation (low solvent viscosity and high diffusivity of the polymer molecules), a normal length SEC column can be used at high flow rate with little loss in resolution.
Im
Kyuhyun
K
Department of Chemistry and BK 21 School of Molecular Science, Pohang University of Science and Technology, Nam-Gu, Pohang, South Korea.
Park
Hae-Woong
HW
Lee
Sekyung
S
Chang
Taihyun
T
D002850:Chromatography, Gel / Q000379:methods*; D002853:Chromatography, Liquid / Q000379:methods*; D006358:Hot Temperature; D011108:Polymers / Q000032:analysis*; D013056:Spectrophotometry, Ultraviolet
D011108:Polymers