Protective effects of squid ink extract towards hemopoietic injuries induced by cyclophosphamine.
To investigate the protective effects of squid ink in chemotherapy, BALB/c mice were used as animal models of injuries induced by cyclophosphamine, a well known chemotherapeutic drug. The mice were randomly divided into five groups with the same number of males and females in each group. At the end of the experiment, animals were sacrificed to investigate organ indexes and antioxidant ability of the spleen, peripheral blood profile and quantities of bone marrow nucleated cells. Results showed that the hemopoietic function of mice was injured by cyclophosphamine, as indicated by decreases of contents of erythrocytes, leukocytes, hemoglobin and bone marrow nucleated cells (P<0.01), while platelets were not affected (P>0.05), as well as modification of organ indexes (P<0.05) and spleen antioxidant ability (P<0.05 or P<0.01), whereas sepia extract markedly increased the levels of erythrocytes, leukocytes, hemoglobin and bone marrow nucleated cells (P<0.01), but not platelets (P>0.05), and reversed the effects of cyclophosphamine on organ indexes and antioxidant ability of spleen (P<0.01 or P<0.05). In addition, squid ink extract did not change marrow hemopoiesis but improved the antioxidant ability of spleen in the animals. The data suggest that squid ink extract can protect the hemopoietic system from chemotherapeutic injury and could be employed to develop cell-protective drugs for use in clinical treatment of tumours.
Zhong
Jie-Ping
JP
Modern Biochemistry Center, Guangdong Ocean University, Zhanjiang 524088, PR China.
Wang
Guang
G
Shang
Jiang-Hua
JH
Pan
Jiang-Qiu
JQ
Li
Kun
K
Huang
Yan
Y
Liu
Hua-Zhong
HZ
D000818:Animals; D000975:Antioxidants / Q000737:chemistry* / Q000627:therapeutic use; D001688:Biological Products / Q000737:chemistry* / Q000627:therapeutic use; D001772:Blood Cell Count; D001773:Blood Cells / Q000166:cytology / Q000187:drug effects; D001854:Bone Marrow Cells / Q000166:cytology / Q000187:drug effects; D003520:Cyclophosphamide / Q000009:adverse effects*; D019610:Cytoprotection; D004305:Dose-Response Relationship, Drug; D005260:Female; D006410:Hematopoiesis / Q000187:drug effects*; D006413:Hematopoietic System / Q000166:cytology / Q000187:drug effects*; D006454:Hemoglobins / Q000032:analysis; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D011897:Random Allocation; D049833:Sepia / Q000737:chemistry*; D013154:Spleen / Q000166:cytology / Q000187:drug effects / Q000378:metabolism
D000975:Antioxidants; D001688:Biological Products; D006454:Hemoglobins; D003520:Cyclophosphamide