Role of MAP kinases in nitric oxide induced muscle-derived adult stem cell apoptosis.
Apoptosis in heart failure has been intensively investigated in vitro and in vivo. Stem cells have therapeutic value in the direct treatment of diseases, including cardiovascular disease. The main drawback of stem cell therapy is their poor survival in the diseased tissues. Since intracellular mitogen-activated protein kinases (MAPKs) actively participate in the regulation of cell survival and of proapoptotic signals, the ability to manipulate the mechanisms of MAPKs activation in myogenic stem cells might increase the survival of transplanted stem cells. Our results clearly demonstrate sustained activation of all three MAPKs, ERK, JNK and p38 in myogenic stem cells after exposure to the NO inducer, NOC-18. Inhibition of MAPKs phosphorylation by specific inhibitors revealed the anti-apoptotic role of MAPKs in myogenic stem cells.
Department of Developmental Biology, Institute of Biochemistry, Vilnius, Lithuania. daiva@bchi.lt
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Bironaite
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Baltriukiene
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Uralova
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Stulpinas
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Bukelskiene
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Imbrasaite
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Kalvelyte
D053687:Adult Stem Cells / Q000166:cytology*; D000818:Animals; D017209:Apoptosis; D020928:Mitogen-Activated Protein Kinases / Q000037:antagonists & inhibitors / Q000378:metabolism / Q000502:physiology*; D009132:Muscles / Q000166:cytology*; D020030:Nitric Oxide Donors / Q000494:pharmacology*; D009603:Nitroso Compounds / Q000494:pharmacology*; D010766:Phosphorylation; D047428:Protein Kinase Inhibitors / Q000494:pharmacology; D016755:Proto-Oncogene Proteins c-jun / Q000378:metabolism; D011817:Rabbits; D015398:Signal Transduction
C093285:NOC 18; D020030:Nitric Oxide Donors; D009603:Nitroso Compounds; D047428:Protein Kinase Inhibitors; D016755:Proto-Oncogene Proteins c-jun; D020928:Mitogen-Activated Protein Kinases