Reverse genetics of Nipah virus to probe viral pathogenicity.
Enhanced green fluorescent protein (EGFP) is a useful marker protein which enables the tracing of virus infection. Recombinant viruses expressing EGFP are useful for the investigation of the underlying mechanism of viral infection in vitro and in vivo. Using EGFP-expressing recombinant Nipah virus (NiV) and canine distemper virus (CDV), we tested the susceptibility of a variety of cells to infection. Receptor usage in CDV infection was also investigated.
The University of Tokyo, Institute of Medical Science, 4-6-1 Shirokanedai, Minato-ku, Tokyo, 108-8639, Japan.
Misako
M
Yoneda
Kentaro
K
Fujita
Hiroki
H
Sato
Chieko
C
Kai
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D002460:Cell Line; D004217:Distemper Virus, Canine / Q000235:genetics / Q000472:pathogenicity; D017930:Genes, Reporter / Q000235:genetics; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D006801:Humans; D045405:Nipah Virus / Q000235:genetics* / Q000472:pathogenicity*; D009928:Organ Specificity; D010957:Plasmids / Q000235:genetics; D011991:Receptors, Virus / Q000378:metabolism
D000911:Antibodies, Monoclonal; D011991:Receptors, Virus; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins