Development and characterization of a genotype 4 hepatitis E virus cell culture system using a HE-JF5/15F strain recovered from a fulminant hepatitis patient.
We developed an efficient cell culture system for genotype 4 hepatitis E virus using the HE-JF5/15F strain recovered from a fulminant hepatitis patient. The sixth-passage virus in the culture supernatant reached 1.5 x 10(8) copies/ml at 10 days postinoculation and possessed 10 nucleotide mutations with four amino acid changes.
Tanaka
Toshinori
T
Division of Virology, Department of Infection and Immunity, Jichi Medical University School of Medicine, 3311-1 Yakushiji, Shimotsuke-Shi, Tochigi-Ken 329-0498, Japan.
Takahashi
Masaharu
M
Takahashi
Hideyuki
H
Ichiyama
Koji
K
Hoshino
Yu
Y
Nagashima
Shigeo
S
Mizuo
Hitoshi
H
Okamoto
Hiroaki
H
D019943:Amino Acid Substitution / Q000235:genetics; D018929:Cell Culture Techniques / Q000379:methods; D002460:Cell Line; D005838:Genotype; D016751:Hepatitis E / Q000821:virology*; D016752:Hepatitis E virus / Q000235:genetics / Q000254:growth & development* / Q000302:isolation & purification*; D006801:Humans; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D020125:Mutation, Missense; D012367:RNA, Viral / Q000235:genetics; D017422:Sequence Analysis, DNA
D012367:RNA, Viral