Multiplexed sandwich immunoassays using electrochemiluminescence imaging resolved at the single bead level.
A new class of bead-based microarray that uses electrogenerated chemiluminescence (ECL) as a readout mechanism to detect multiple antigens simultaneously is presented. This platform demonstrates the possibility of performing highly multiplexed assays using ECL because all the individual sensing beads in the array are simultaneously imaged and individually resolved by ECL. Duplex and triplex assay results are demonstrated as well as a cross reactivity study.
Deiss
Frédérique
F
Universite Bordeaux, Institut des Sciences Moleculaires, ENSCPB, 16 Avenue Pey-Berland, 33607 Pessac, France.
LaFratta
Christopher N
CN
Symer
Matthew
M
Blicharz
Timothy M
TM
Sojic
Neso
N
Walt
David R
DR
D000941:Antigens / Q000032:analysis; D003429:Cross Reactions; D004563:Electrochemistry / Q000379:methods*; D007118:Immunoassay / Q000379:methods*; D008163:Luminescent Measurements / Q000379:methods*; D008863:Microspheres; D040081:Protein Array Analysis / Q000379:methods*
D000941:Antigens