Focal electroporation in zebrafish embryos and larvae.
A method is described that allows the introduction by electroporation of either small dyes or larger RNA, DNA, or morpholino constructs into single cells or small groups of cells in zebrafish embryos or larvae. The dye or construct is delivered to cells via a patch-like microelectrode that also delivers the electroporation stimulus train. This technique allows the experimenter to target cells of their choice at a particular time of development and at a particular location in the embryo, and is useful for fate mapping, analysing neuronal organisation, ectopic expression and gene knockdown experiments.
MRC Centre for Developmental Neurobiology, King's College London, New Hunt's House, 4th Floor, Guy's Hospital Campus, London SE1 1UL, UK.
Marcel
M
Tawk
Isaac H
IH
Bianco
Jonathan D W
JD
Clarke
D000818:Animals; D030801:Animals, Genetically Modified / Q000196:embryology / Q000235:genetics / Q000378:metabolism; D004247:DNA / Q000235:genetics / Q000378:metabolism; D015509:Developmental Biology / Q000379:methods*; D018274:Electroporation / Q000379:methods*; D004625:Embryo, Nonmammalian / Q000166:cytology / Q000187:drug effects; D005456:Fluorescent Dyes / Q000494:pharmacology; D007814:Larva / Q000166:cytology / Q000187:drug effects; D008853:Microscopy; D012313:RNA / Q000235:genetics / Q000378:metabolism; D012235:Rhodamines / Q000494:pharmacology; D013997:Time Factors; D015027:Zebrafish / Q000196:embryology* / Q000235:genetics* / Q000378:metabolism
D005456:Fluorescent Dyes; D012235:Rhodamines; C005358:tetramethylrhodamine; D012313:RNA; D004247:DNA