Induction of type 1 interferon receptor by zinc in U937 cells.
This study aims to determine whether zinc enhances interferon (IFN)-alpha activity in U937 cells. Type 1 IFN2 receptor (IFNAR2) protein in U937 cells was measured by flow cytometry. After 24h of exposure to zinc chloride or polaprezinc (a chelate of zinc and L-carnosine) at concentrations ranging from 50 to 200 microM, histograms showing anti-IFNAR2 antibody-positive cells shifted to a higher FITC intensity. Zinc chloride and polaprezinc increased IFNAR2 mRNA levels approximately 30% and 40%, respectively, compared to the control. L-carnosine alone did not alter IFNAR2 mRNA or protein levels. Cellular levels of 2'-5' oligoadenylate synthetases (OAS) were markedly increased by IFN-alpha, and the increase was significantly accelerated by polaprezinc. However, polaprezinc alone did not increase 2'-5'OAS levels. The finding suggests that zinc, especially polaprezinc, enhances the expression of INFAR2 in U937 cells, thereby inducing production of the anti-viral protein 2'-5'OAS.
School of Health Science, Faculty of Medicine, Gunma University, 3-39-15 Showa-machi, Maebashi, Gunma 371-8514, Japan. mine@health.gunma-u.ac.jp
Takeaki
T
Nagamine
Kastuyuki
K
Nakajima
Hisashi
H
Takada
Yoshitaka
Y
Sekine
Kazuhiro
K
Suzuki
D015088:2',5'-Oligoadenylate Synthetase / Q000378:metabolism; D000897:Anti-Ulcer Agents / Q000494:pharmacology; D002336:Carnosine / Q000031:analogs & derivatives* / Q000494:pharmacology; D002712:Chlorides / Q000494:pharmacology*; D006801:Humans; D016898:Interferon-alpha / Q000378:metabolism; D009942:Organometallic Compounds / Q000494:pharmacology*; D053633:Receptor, Interferon alpha-beta / Q000235:genetics / Q000378:metabolism*; D020298:U937 Cells / Q000187:drug effects / Q000378:metabolism; D017967:Zinc Compounds / Q000494:pharmacology*
D000897:Anti-Ulcer Agents; D002712:Chlorides; C508033:IFNAR2 protein, human; D016898:Interferon-alpha; D009942:Organometallic Compounds; D017967:Zinc Compounds; C061957:polaprezinc; D053633:Receptor, Interferon alpha-beta; C016837:zinc chloride; D002336:Carnosine; D015088:2',5'-Oligoadenylate Synthetase