A novel inhibitory effect of Antrodia camphorata extract on vascular smooth muscle cell migration and neointima formation in mice.
Antrodia camphorata (AC) is a well-known traditional Chinese medicine that has been shown to inhibit proliferation and migration of cancer cells. We examined whether AC could inhibit rat aortic smooth muscle cell (RASMC) proliferation and migration and evaluated its effect on neointima formation in mouse carotid artery after injury. In Transwell migration assay and wound scratch assay, RASMCs were treated with AC or saline, and the number of migrated cells was counted or the distance was determined. Both assays showed that AC significantly inhibited platelet-derived growth factor (PDGF)-induced SMC migration. In 3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) and 5-bromo-2' deoxyuridine (BrdU) proliferation assays, RASMCs were pretreated with AC or saline and stimulated with PDGF. Both assays showed that AC inhibited PDGF-induced SMC proliferation. The left common carotid arteries of C57BL/6 mice were ligated near the carotid bifurcation. The mice were given water or AC for 4 weeks. The severity of neointima formation was expressed as the neointima/media (N/M) ratio. The AC-treated mice had less neointima formation at 4 weeks after carotid ligation (N/M ratio, water versus 250 versus 1250 mg/kg AC; 1.33 +/- 0.87 versus 0.83 +/- 0.45 versus 0.63 +/- 0.32, P < 0.05).Our data indicate that AC is an effective inhibitor of PDGF-induced RASMC proliferation and migration. AC treatment reduced neointima formation in this mouse carotid ligation model.
Department of Internal Medicine, National Cheng Kung University College of Medicine and Hospital, Taiwan, Republic of China.
Yi-Heng
YH
Li
Hsing-Chun
HC
Chung
Shu-Lin
SL
Liu
Ting-Hsing
TH
Chao
Jian-Chyi
JC
Chen
D000465:Algorithms; D000818:Animals; D055491:Antrodia / Q000737:chemistry*; D001011:Aorta; D020212:Carotid Artery Injuries / Q000188:drug therapy*; D002465:Cell Movement / Q000187:drug effects; D002478:Cells, Cultured; D004195:Disease Models, Animal; D048690:Fruiting Bodies, Fungal / Q000737:chemistry; D066298:In Vitro Techniques; D008297:Male; D008516:Medicine, Chinese Traditional; D051379:Mice; D008810:Mice, Inbred C57BL; D009131:Muscle, Smooth, Vascular / Q000187:drug effects* / Q000473:pathology; D032389:Myocytes, Smooth Muscle / Q000187:drug effects*; D009369:Neoplasms / Q000188:drug therapy; D009389:Neovascularization, Pathologic / Q000517:prevention & control*; D010982:Platelet-Derived Growth Factor / Q000187:drug effects*; D051381:Rats; D017539:Tunica Intima / Q000187:drug effects*
D010982:Platelet-Derived Growth Factor