Nicotine induced modulation of SLURP-1 expression in human colon cancer cells.
The secreted mammalian Ly-6/urokinase plasminogen activator receptor-related protein-1 (SLURP-1) is an endogenous ligand at the alpha 7 subunit of the nicotinic acetylcholine receptor (nAChR). SLURP-1 has anti-tumourigenic properties. In the current study, we demonstrate that the challenge of HT-29 human colon cancer cells with nicotine for 24 h to increase cell growth via the alpha 7nAChRs, caused a marked reduction of the protein expression of SLURP-1. We suggest that there is an interplay between acetylcholine and SLURP-1 in the HT-29 cells, both molecules serving as autocrine growth controlling ligands at the alpha 7nAChR, where acetylcholine regulates the release of SLURP-1.
School of Pure and Applied Natural Sciences, University of Kalmar, Kalmar, Sweden. ann.pettersson@hik.se
Ann
A
Pettersson
Gunnar
G
Nylund
Amir
A
Khorram-Manesh
Svante
S
Nordgren
Dick S
DS
Delbro
D000950:Antigens, Ly / Q000378:metabolism*; D045744:Cell Line, Tumor; D003110:Colonic Neoplasms / Q000378:metabolism* / Q000473:pathology; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects*; D006801:Humans; D009538:Nicotine / Q000494:pharmacology*; D018722:Nicotinic Agonists / Q000494:pharmacology*; D014568:Urokinase-Type Plasminogen Activator / Q000378:metabolism*
D000950:Antigens, Ly; D018722:Nicotinic Agonists; C048963:SLURP1 protein, human; D009538:Nicotine; D014568:Urokinase-Type Plasminogen Activator