Measurement of platelet-derived microparticle levels using an enzyme-linked immunosorbent assay in polymyositis and dermatomyositis patients.
Platelet-derived microparticle (PDMP) levels were measured using an enzyme-linked immunosorbent assay (ELISA) to elucidate the role of platelet activation in patients with polymyositis or dermatomyositis (PM/DM). PDMP levels in active PM/DM patients (median 13.3 U/ml, interquartile range 9.9-20.7 U/ml, n = 16) and those in patients undergoing treatment (12.1 U/ml, 7.4-16.7 U/ml, n = 12) were significantly higher than in controls (6.5 U/ml, 5.0-8.4 U/ml, n = 26, vs. active, P = 0.0001; vs. treatment, P = 0.004). In a paired sampling study, PDMP decreased significantly after glucocorticoid treatment (P = 0.04). PDMP in the active PM/DM patients correlated significantly with serum C-reactive protein levels (r(s) = 0.67, P = 0.01). These results suggest that platelets may play an important role in the inflammatory process, and that PDMP level could be a useful marker of inflammatory activity in PM/DM patients. Muscle Nerve 39: 586-590, 2009.
Division of Neurology, Department of Internal Medicine, Kobe University Graduate School of Medicine, 7-5-2 Kusunoki-cho, Chuo-ku, Kobe 650-0017, Japan. 056d523m@stu.kobe-u.ac.jp
Toshihiko
T
Shirafuji
Hirotoshi
H
Hamaguchi
Masatsugu
M
Higuchi
Fumio
F
Kanda
D000328:Adult; D000368:Aged; D002097:C-Reactive Protein / Q000378:metabolism; D055252:Cell-Derived Microparticles / Q000378:metabolism*; D003402:Creatine Kinase / Q000097:blood; D003882:Dermatomyositis / Q000097:blood* / Q000188:drug therapy; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005260:Female; D005938:Glucocorticoids / Q000627:therapeutic use; D006801:Humans; D008297:Male; D008875:Middle Aged; D017285:Polymyositis / Q000097:blood* / Q000188:drug therapy; D018709:Statistics, Nonparametric
D005938:Glucocorticoids; D002097:C-Reactive Protein; D003402:Creatine Kinase