Mechanism of the very efficient quenching of tryptophan fluorescence in human gamma D- and gamma S-crystallins: the gamma-crystallin fold may have evolved to protect tryptophan residues from ultraviolet photodamage.
Proteins exposed to UV radiation are subject to irreversible photodamage through covalent modification of tryptophans (Trps) and other UV-absorbing amino acids. Crystallins, the major protein components of the vertebrate eye lens that maintain lens transparency, are exposed to ambient UV radiation throughout life. The duplicated beta-sheet Greek key domains of beta- and gamma-crystallins in humans and all other vertebrates each have two conserved buried Trps. Experiments and computation showed that the fluorescence of these Trps in human gammaD-crystallin is very efficiently quenched in the native state by electrostatically enabled electron transfer to a backbone amide [Chen et al. (2006) Biochemistry 45, 11552-11563]. This dispersal of the excited state energy would be expected to minimize protein damage from covalent scission of the excited Trp ring. We report here both experiments and computation showing that the same fast electron transfer mechanism is operating in a different crystallin, human gammaS-crystallin. Examination of solved structures of other crystallins reveals that the Trp conformation, as well as favorably oriented bound waters, and the proximity of the backbone carbonyl oxygen of the n - 3 residues before the quenched Trps (residue n), are conserved in most crystallins. These results indicate that fast charge transfer quenching is an evolved property of this protein fold, probably protecting it from UV-induced photodamage. This UV resistance may have contributed to the selection of the Greek key fold as the major lens protein in all vertebrates.
Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA.
Jiejin
J
Chen
Patrik R
PR
Callis
Jonathan
J
King
D003459:Crystallins / Q000737:chemistry* / Q000235:genetics / Q000528:radiation effects; D018360:Crystallography, X-Ray; D004579:Electron Transport / Q000235:genetics / Q000528:radiation effects; D019143:Evolution, Molecular; D006801:Humans; D007908:Lens, Crystalline / Q000737:chemistry* / Q000293:injuries / Q000528:radiation effects; D016296:Mutagenesis; D017510:Protein Folding / Q000528:radiation effects; D013050:Spectrometry, Fluorescence; D055672:Static Electricity; D013472:Sunlight / Q000009:adverse effects; D014364:Tryptophan / Q000737:chemistry* / Q000235:genetics / Q000528:radiation effects; D014466:Ultraviolet Rays / Q000009:adverse effects*; D038222:gamma-Crystallins / Q000737:chemistry* / Q000235:genetics / Q000528:radiation effects
C504903:CRYGD protein, human; D003459:Crystallins; D038222:gamma-Crystallins; C077648:CRYGS protein, human; D014364:Tryptophan