The RNA aptamer disrupts protein-protein interaction between beta-catenin and nuclear factor-kappaB p50 and regulates the expression of C-reactive protein.
Transcription is activated by signal-induced protein-protein interaction between transcription factors on regulatory elements positioned near their target genes. Here, we tested the utility of the beta-catenin binding RNA aptamer as a tool for studying protein-protein interaction within transcription complex and for modulating expression of a target gene. The RNA aptamer bound Armadillo repeats of beta-catenin and was effective in disrupting protein-protein interaction between beta-catenin and nuclear factor-kappaB (NF-kappaB) p50. In addition, the RNA aptamer effectively reduced tumor necrosis factor-alpha induced transcription from the promoter of C-reactive protein regulated by beta-catenin and NF-kappaB p50. Taken together, beta-catenin binding RNA aptamer was an effective regulator of beta-catenin and NF-kappaB p50 mediated transcription.
National Research Laboratory for RNA Cell Biology, BK21 Graduate Program for RNA Biology and Department of Molecular Biology, Dankook University, 126, Yongin-si, Gyeonggi-do 448-701, Republic of Korea.
Yong Seok
YS
Choi
Jung
J
Hur
Hee Kyu
HK
Lee
Sunjoo
S
Jeong
D052157:Aptamers, Nucleotide; D001483:Base Sequence; D015152:Blotting, Northern; D002097:C-Reactive Protein / Q000378:metabolism*; D002460:Cell Line; D006801:Humans; D047468:Immunoprecipitation; D008969:Molecular Sequence Data; D052002:NF-kappa B p50 Subunit / Q000378:metabolism*; D009690:Nucleic Acid Conformation; D011485:Protein Binding; D020133:Reverse Transcriptase Polymerase Chain Reaction; D051176:beta Catenin / Q000378:metabolism*
D052157:Aptamers, Nucleotide; D052002:NF-kappa B p50 Subunit; D051176:beta Catenin; D002097:C-Reactive Protein