Metabolite interference in pharmacokinetic studies.
Our policy of conducting biotransformation studies with extended chromatography prior to pharmacokinetic bioanalyses allowed us to quickly detect an unusual, cis/trans metabolite in rat plasma that was inseparable using a short chromatographic method. We caution investigators that short methods invite unknown isobaric metabolites to cause inaccuracies in plasma concentration measurements.
Celgene, 4550 Towne Centre Ct, San Diego, CA 92121, USA.
Mehran F
MF
Moghaddam
Jui-Hsiang
JH
Fan
Yang
Y
Tang
Oleg
O
Khatsenko
Jason C
JC
Katz
Michael A
MA
Shirley
D000818:Animals; D001711:Biotransformation; D002853:Chromatography, Liquid / Q000379:methods*; D003511:Cyclohexanols / Q000378:metabolism*; D055808:Drug Discovery / Q000379:methods; D005260:Female; D022781:Hepatocytes / Q000378:metabolism; D008297:Male; D004364:Pharmaceutical Preparations / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013237:Stereoisomerism
D003511:Cyclohexanols; D004364:Pharmaceutical Preparations