Predicting Toll-like receptor structures and characterizing ligand binding.
Toll-like receptor (TLR) ligand-binding domains comprise 18-25 tandem copies of a 24-residue motif known as the leucine-rich repeat (LRR). Unlike other LRR proteins, TLRs contain significant numbers of non-consensus LRR sequences, which makes their identification by computer domain search programs problematic. Here, we provide methods for identifying non-consensus LRRs. Using the location of these LRRs, hypothetical models are constructed based on the known molecular structures of homologous LRR proteins. However, when a hypothetical model for TLR3 is compared with the molecular structure solved by x-ray crystallography, the solenoid curvature, planarity, and conformations of the LRR insertions are incorrectly predicted. These differences illustrate how non-consensus LRR motifs influence TLR structure. Since the determination of molecular structures by crystallography requires substantial amounts of protein, we describe methods for producing milligram amounts of TLR3 extracellular domain (ECD) protein. The recombinant TLR3-ECD previously used to solve the molecular structure of TLR3-ECD has also been used to study the binding of TLR3-ECD to its ligand, double-stranded RNA (dsRNA). In the last section, we describe the preparation of defined TLR3 ligands and present methods for characterizing their interaction with TLR3-ECD.
Experimental Immunology Branch, NCI, NIH, Bethesda, MD 20892-1360, USA.
Joshua N
JN
Leonard
Jessica K
JK
Bell
David M
DM
Segal
D000818:Animals; D004797:Enzyme-Linked Immunosorbent Assay; D005110:Extracellular Space / Q000737:chemistry / Q000378:metabolism; D008024:Ligands; D051379:Mice; D011485:Protein Binding; D051193:Toll-Like Receptors / Q000032:analysis* / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism
D008024:Ligands; D051193:Toll-Like Receptors