Embryonic stem cell-specific microRNAs promote induced pluripotency.
This report demonstrates that introduction of microRNAs (miRNAs) specific to embryonic stem cells enhances the production of mouse induced pluripotent stem (iPS) cells. The miRNAs miR-291-3p, miR-294 and miR-295 increase the efficiency of reprogramming by Oct4, Sox2 and Klf4, but not by these factors plus cMyc. cMyc binds the promoter of the miRNAs, suggesting that they are downstream effectors of cMyc during reprogramming. However, unlike cMyc, the miRNAs induce a homogeneous population of iPS cell colonies.
Judson
Robert L
RL
The Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research, University of California, San Francisco, San Francisco, California, USA.
Babiarz
Joshua E
JE
Venere
Monica
M
Blelloch
Robert
R
D000818:Animals; D054337:Cell Dedifferentiation; D053595:Embryonic Stem Cells / Q000166:cytology* / Q000378:metabolism*; D000090062:Kruppel-Like Factor 4; D051379:Mice; D035683:MicroRNAs / Q000378:metabolism*; D039904:Pluripotent Stem Cells / Q000378:metabolism; D016271:Proto-Oncogene Proteins c-myc / Q000378:metabolism
C000715468:Klf4 protein, mouse; D000090062:Kruppel-Like Factor 4; D035683:MicroRNAs; D016271:Proto-Oncogene Proteins c-myc