Application of spontaneously immortalized odontoblast cells in tooth regeneration.
Here, we report on the first attempt to bioengineer tooth using a spontaneously immortalized mesenchymal cell line. To assess the odontogenic potential of this cell line, odontoblast-lineage cells (OLC) were re-associated with competent dental epithelium isolated from E14.5 mice. A novel three-dimensional organ germ culture method was applied to nurture the constructs in vitro. Additionally, recombinants were transplanted under the kidney capsule in host animals for 2 weeks. Transplants developed into tooth tissues in one-third of the cases. OLC-derived GFP-positive cells could be identified in mineralizing tooth germs by immunohistochemistry. OLCs were capable of intercellular and cell-matrix communication, thus they eventually differentiated into functional odontoblasts. In summary, we managed to utilize OLCs for dental mesenchyme substitution in tooth regeneration experiments. Therefore, our spontaneously transformed cell line proved its potential for future complex, tooth developmental and bioengineering studies.
Department of Biochemistry, Akita University School of Medicine, Hondo 1-1-1, Akita 010-8543, Japan. aszilvia@med.akita-u.ac.jp
Szilvia
S
Arany
Masami
M
Kawagoe
Toshihiro
T
Sugiyama
D000818:Animals; D018929:Cell Culture Techniques; D002461:Cell Line, Transformed; D019070:Cell Lineage; D051379:Mice; D008813:Mice, Inbred ICR; D009804:Odontoblasts / Q000166:cytology / Q000502:physiology*; D009805:Odontogenesis; D012038:Regeneration; D023822:Tissue Engineering / Q000379:methods*; D014070:Tooth / Q000166:cytology / Q000502:physiology*