Large-scale small-molecule screen using zebrafish embryos.
Zebrafish represents a versatile model organism with many molecular, morphological, and physiological similarities to mammals. Importantly, zebrafish are readily susceptible to perturbations by small molecules, including numerous pharmaceuticals in clinical use. Given these qualities, plus their small size and transparency, zebrafish embryos can be utilized for large-scale phenotype-based screens for small-molecule modifiers of biological processes. Thus, in a manner analogous to classical genetic screens, zebrafish chemical screens have the potential to reveal novel insights into complex biological pathways, as well as to identify lead compounds for novel therapeutics.
Hong
Charles C
CC
Division of Cardiovascular Medicine, Department of Pharmacology, Vanderbilt University School of Medicine, 383 Preston Research Building, 2220 Pierce Avenue, Nashville, TN 37232, USA. charles.c.hong@vanderbilt.edu
D000818:Animals; D004353:Drug Evaluation, Preclinical / Q000379:methods*; D004625:Embryo, Nonmammalian / Q000187:drug effects* / Q000378:metabolism; D054852:Small Molecule Libraries / Q000494:pharmacology*; D015027:Zebrafish / Q000196:embryology* / Q000235:genetics
D054852:Small Molecule Libraries