Homocysteine levels in the vitreous of proliferative diabetic retinopathy and rhegmatogenous retinal detachment: its modulating role on lysyl oxidase.
OBJECTIVE
Homocysteine (Hcys), a well-known inducer of vascular endothelial cell damage has been associated with extracellular matrix changes. Lysyl oxidase (LOX) is a copper-dependent amine oxidase that initiates the covalent cross-linking of collagen and elastin in the extracellular matrix (ECM). LOX contributes to the structural integrity of the ECM, and low LOX activity could promote ECM disorganization. Hydroxyproline levels are used to predict collagen turnover status, and most of the endogenous hydroxyproline present in biological fluids is derived from the degradation of various forms of collagen. As Hcys is known to regulate ECM turnover and also inhibit LOX activity, the purpose of this study was to estimate the vitreous levels of Hcys in eyes with proliferative diabetic retinopathy (PDR) and rhegmatogenous retinal detachment (RRD) and to correlate the effect of Hcys, if any on LOX activity.


METHODS
Undiluted human vitreous specimens obtained during vitreoretinal surgeries for PDR (n = 18) and RRD (n = 17) were used. Vitreous specimens from donor eyeballs were used as control (n = 19). Hcys was estimated by HPLC using a fluorescent detector. Hydroxyproline was estimated spectrophotometrically.


RESULTS
The total vitreous Hcys level was found to be increased significantly in PDR (P = 0.011) and in RRD (P = 0.001) compared with that in control samples. Hydroxyproline was significantly increased in PDR (P = 0.049) and RRD (P = 0.007) compared with the level in control samples. There was a significant negative correlation between the Hcys level and the specific activity of LOX in PDR (P = 0.040) and in RRD (P = 0.029)


CONCLUSIONS
This report shows that increased vitreous Hcys in PDR and RRD is associated with a significant decrease in LOX-specific activity along with an increase in collagen turnover.
Coral
Karunakaran
K
Biochemistry Research Department, Shri Bhagwan Mahavir Vitreoretinal Services, Vision Research Foundation, Sankara Nethralaya, Chennai, India.
Angayarkanni
Narayanasamy
N
Gomathy
Narayanan
N
Bharathselvi
Muthuvel
M
Pukhraj
Rishi
R
Rupak
Roy
R
D000328:Adult; D000368:Aged; D002851:Chromatography, High Pressure Liquid; D003094:Collagen / Q000378:metabolism; D003930:Diabetic Retinopathy / Q000378:metabolism*; D005260:Female; D006710:Homocysteine / Q000378:metabolism*; D006801:Humans; D006909:Hydroxyproline / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D008249:Protein-Lysine 6-Oxidase / Q000378:metabolism*; D012163:Retinal Detachment / Q000378:metabolism*; D014822:Vitreous Body / Q000378:metabolism*; D055815:Young Adult
D006710:Homocysteine; D003094:Collagen; D008249:Protein-Lysine 6-Oxidase; D006909:Hydroxyproline