Myelin-associated glycoprotein inhibits the neuronal differentiation of neural progenitors.
Nogo-66, myelin-associated glycoprotein (MAG), and oligodendrocyte myelin glycoprotein, possess axon growth-inhibiting properties by binding with the Nogo-66 receptor. Recent studies have shown that Nogo-66 inhibits neuronal differentiation of neural progenitor cells (NPCs) and the neurite outgrowth of the neurons differentiated from NPCs. However, the effects of MAG on the differentiation and proliferation of NPCs are unclear. We found that NPCs derived from the hippocampus of embryonic rats expressed Nogo-66 receptor and MAG-Fc, which mimics the function of MAG, inhibited the differentiation of NPCs into neurons but promoted differentiation of NPCs into astrocytes. Furthermore, MAG-Fc inhibited the neurite outgrowth of the neurons differentiated from NPCs. Our results suggest that MAG can inhibit the neuronal differentiation of NPCs.
Li
Xin
X
Department of Emergency, The First Affiliated Hospital of Sun Yat-Sen University, Guangzhou, China.
Fu
Qing-Ling
QL
Jing
Xiao-Li
XL
Liao
Xin-Xue
XX
Zeng
Ai-Hong
AH
Xiong
Yan
Y
Liao
Xiao-Xing
XX
D000704:Analysis of Variance; D000818:Animals; D001253:Astrocytes / Q000502:physiology; D002452:Cell Count; D002454:Cell Differentiation; D002478:Cells, Cultured; D058851:GPI-Linked Proteins; D006624:Hippocampus / Q000166:cytology / Q000502:physiology; D007150:Immunohistochemistry; D009185:Myelin Proteins / Q000378:metabolism; D018992:Myelin-Associated Glycoprotein / Q000378:metabolism*; D016501:Neurites / Q000502:physiology; D055495:Neurogenesis / Q000502:physiology*; D009474:Neurons / Q000166:cytology* / Q000502:physiology; D000070816:Nogo Receptor 1; D051381:Rats; D017207:Rats, Sprague-Dawley; D011956:Receptors, Cell Surface / Q000378:metabolism; D013234:Stem Cells / Q000166:cytology* / Q000502:physiology
D058851:GPI-Linked Proteins; D009185:Myelin Proteins; D018992:Myelin-Associated Glycoprotein; D000070816:Nogo Receptor 1; D011956:Receptors, Cell Surface; C486581:Rtn4r protein, rat