Effect of microphase separation on the protein resistance of a polymeric surface.
Segmented polyurethanes (PUs) containing poly(ethylene glycol) (PEG), poly(propylene glycol), or poly(dimethylsiloxane) soft segments have been prepared by two-step condensation polymerization. Atom force microscopy observation in air and solution indicates that the segmented PU forms a microphase separation on the surface. By use of quartz crystal microbalance with dissipation and surface plasmon resonance, we have investigated the adsorption of fibrinogen, bovine serum albumin, and lysozyme on a surface constructed by such a PU in aqueous solution in real time. Our results reveal that the protein resistance of the PUs arises from the hydrated PEG segments instead of microphase separation.
Ma
Chunfeng
C
Hefei National Laboratory for Physical Sciences at Microscale, Department of Chemical Physics, University of Science and Technology of China, Hefei 230026, China.
Hou
Yi
Y
Liu
Shi
S
Zhang
Guangzhao
G
D000042:Absorption; D000818:Animals; D002417:Cattle; D004129:Dimethylpolysiloxanes / Q000737:chemistry; D008832:Microchemistry; D018625:Microscopy, Atomic Force; D015394:Molecular Structure; D044367:Phase Transition; D011092:Polyethylene Glycols / Q000737:chemistry; D011108:Polymers / Q000737:chemistry; D011140:Polyurethanes / Q000737:chemistry*; D011409:Propylene Glycols / Q000737:chemistry; D011506:Proteins / Q000032:analysis / Q000737:chemistry*; D012710:Serum Albumin, Bovine; D013499:Surface Properties
D004129:Dimethylpolysiloxanes; D011108:Polymers; D011140:Polyurethanes; D011409:Propylene Glycols; D011506:Proteins; C012504:polypropylene glycol; D012710:Serum Albumin, Bovine; D011092:Polyethylene Glycols; C013830:baysilon