Early energy and protein reduction: effects on growth, blood profiles and expression of genes related to protein and fat metabolism in broilers.
1. A total of 320-d-old Ross broilers were used in a 6-week study to investigate the effects of feeding lower energy and protein diets from d 8 to 14 on growth performance, blood profiles, and gene expression of leptin and myostatin. 2. Broilers were randomly allotted to 4 treatments, each treatment applied to 4 pens with 20 birds in each. During first week, all the birds were fed on a common starter diet (13.4 MJ ME/kg, 230 g/kg CP and 11.0 g/kg lysine). The birds were then subjected to their respective treatment diets from d 8 to 14. Treatment diets comprised two ME levels, 13.4 and 12.0 MJ/kg, each with two levels of CP, 230 and 184 g/kg. This was followed by feeding common starter and finisher diets for the last 4 weeks. 3. Dietary protein reduction resulted in poor performance and feed efficiency while energy reduction resulted in poor feed efficiency between d 8 and 14. From d 14 to 42 birds previously fed diets lower in energy and protein showed similar body weight gain and feed intake to well-fed birds. Moreover from d 8 to 14, birds fed on energy and protein-reduced diets had lower nutrient metabolisability coefficients. 4. The blood urea nitrogen (BUN) and relative weights of heart and breast muscle were lower in birds fed protein-reduced diets while energy reduction resulted in lower plasma glucose, abdominal fat and intestinal weight at d 14. At d 42, birds fed on the protein-reduced diets had lower BUN, breast muscle weight and small intestine length, while feeding on the energy-reduced diets resulted in lower abdominal fat. 5. Upregulated myostatin mRNA expression in breast muscle and downregulation of leptin mRNA expression in abdominal fat were observed in birds fed on protein and energy-reduced diets, respectively. 6. In conclusion, early nutrient reduction affected growth performance and produced lesser abdominal fat in broilers. Moreover, early energy and/or protein reduction could change muscle and fat metabolism by regulating the expressions of myostatin and leptin.
Yang
Y X
YX
College of Animal Life Sciences, Kangwon National University, Chuncheon, Republic of Korea.
Guo
J
J
Yoon
S Y
SY
Jin
Z
Z
Choi
J Y
JY
Piao
X S
XS
Kim
B W
BW
Ohh
S J
SJ
Wang
M H
MH
Chae
B J
BJ
D000596:Amino Acids / Q000378:metabolism; D000818:Animals; D001806:Blood Urea Nitrogen; D031204:Caloric Restriction / Q000662:veterinary*; D002645:Chickens / Q000097:blood* / Q000235:genetics / Q000254:growth & development*; D018753:Diet, Protein-Restricted / Q000662:veterinary*; D004041:Dietary Fats / Q000378:metabolism*; D004044:Dietary Proteins / Q000378:metabolism*; D004063:Digestion; D004435:Eating; D015870:Gene Expression; D018698:Glutamic Acid / Q000097:blood; D007082:Ileum / Q000378:metabolism; D020738:Leptin / Q000235:genetics; D018482:Muscle, Skeletal / Q000737:chemistry; D055435:Myostatin / Q000235:genetics; D009929:Organ Size; D015430:Weight Gain
D000596:Amino Acids; D004041:Dietary Fats; D004044:Dietary Proteins; D020738:Leptin; D055435:Myostatin; D018698:Glutamic Acid