Requirements for E1A dependent transcription in the yeast Saccharomyces cerevisiae.
BACKGROUND
The human adenovirus type 5 early region 1A (E1A) gene encodes proteins that are potent regulators of transcription. E1A does not bind DNA directly, but is recruited to target promoters by the interaction with sequence specific DNA binding proteins. In mammalian systems, E1A has been shown to contain two regions that can independently induce transcription when fused to a heterologous DNA binding domain. When expressed in Saccharomyces cerevisiae, each of these regions of E1A also acts as a strong transcriptional activator. This allows yeast to be used as a model system to study mechanisms by which E1A stimulates transcription.


RESULTS
Using 81 mutant yeast strains, we have evaluated the effect of deleting components of the ADA, COMPASS, CSR, INO80, ISW1, NuA3, NuA4, Mediator, PAF, RSC, SAGA, SAS, SLIK, SWI/SNF and SWR1 transcriptional regulatory complexes on E1A dependent transcription. In addition, we examined the role of histone H2B ubiquitylation by Rad6/Bre1 on transcriptional activation.


CONCLUSIONS
Our analysis indicates that the two activation domains of E1A function via distinct mechanisms, identify new factors regulating E1A dependent transcription and suggest that yeast can serve as a valid model system for at least some aspects of E1A function.
Department of Microbiology & Immunology, University of Western Ontario, London, Ontario, Canada. ayousef@uwo.ca
Ahmed F
AF
Yousef
Christopher J
CJ
Brandl
Joe S
JS
Mymryk
D017500:Adenovirus E1A Proteins / Q000235:genetics* / Q000378:metabolism*; D005786:Gene Expression Regulation; D015966:Gene Expression Regulation, Fungal; D017434:Protein Structure, Tertiary; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism; D014157:Transcription Factors / Q000378:metabolism
D017500:Adenovirus E1A Proteins; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors