Carbonic anhydrase inhibitors. Inhibition of the fungal beta-carbonic anhydrases from Candida albicans and Cryptococcus neoformans with boronic acids.
Inhibition of the beta-carbonic anhydrases (CAs, EC 4.2.1.1) from the pathogenic fungi Cryptococcus neoformans (Can2) and Candida albicans (Nce103) with a series of aromatic, arylalkenyl- and arylalkylboronic acids was investigated. Aromatic, 4-phenylsubstituted- and 2-naphthylboronic acids were the best Can2 inhibitors, with inhibition constants in the range of 8.5-11.5microM, whereas arylalkenyl and aryalkylboronic acids showed K(I)s in the range of 428-3040microM. Nce103 showed a similar inhibition profile, with the 4-phenylsubstituted- and 2-naphthylboronic acids possessing K(I)s in the range of 7.8-42.3microM, whereas the arylalkenyl and aryalkylboronic acids were weaker inhibitors (K(I)s of 412-5210microM). The host human enzymes CA I and II were also effectively inhibited by these boronic acids. The B(OH)(2) moiety is thus a new zinc-binding group for designing effective inhibitors of the alpha- and beta-CAs.
Università degli Studi di Firenze, Polo Scientifico, Sesto Fiorentino, Florence, Italy.
Alessio
A
Innocenti
Jean-Yves
JY
Winum
Rebecca A
RA
Hall
Fritz A
FA
Mühlschlegel
Andrea
A
Scozzafava
Claudiu T
CT
Supuran
D001897:Boronic Acids / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology; D002176:Candida albicans / Q000201:enzymology*; D002257:Carbonic Anhydrase Inhibitors / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology; D002256:Carbonic Anhydrases / Q000737:chemistry* / Q000378:metabolism; D003455:Cryptococcus neoformans / Q000201:enzymology*; D006801:Humans; D013329:Structure-Activity Relationship
D001897:Boronic Acids; D002257:Carbonic Anhydrase Inhibitors; D002256:Carbonic Anhydrases