Unexpected reversal of the regioselectivity in Thermomyces lanuginosus lipase-catalyzed acylation of floxuridine.
Unexpected inversion of the 3':5'-regioselectivity was observed in the enzymatic methacryloylation, crotonylation and cinnamoylation of floxuridine (1.5:1, 2.3:1 and 4.4:1, respectively), where Thermomyces lanuginosus lipase preferentially catalyzed the acylation of 3'-hydroxyl rather than that of 5'-hydroxyl group. The possible reason might be the presence of a remote interaction between the unsaturated bond in the acyl group and the aromatic ring of amino acid residue Trp89 in the lid of the lipase.
Lab of Applied Biocatalysis, College of Light Industry and Food Sciences, South China University of Technology, 510640, Guangzhou, China.
Ning
N
Li
Min-Hua
MH
Zong
Ding
D
Ma
D000215:Acylation; D001203:Ascomycota / Q000201:enzymology* / Q000378:metabolism*; D005467:Floxuridine / Q000378:metabolism*; D008049:Lipase / Q000378:metabolism*; D013237:Stereoisomerism; D013379:Substrate Specificity
D005467:Floxuridine; D008049:Lipase