Reliable identification of clinically prevalent species and subspecies of staphylococci by sodium dodecyl sulfate polyacrylamide gel electrophoresis analysis.
We identified by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) 10 species and 5 subspecies of Staphylococcus among 139 clinical isolates and compared it with conventional tests. All isolates showed species-specific whole-cell protein profiles, even atypical strains, with up to 60% and at least 73.5% of interspecies and intraspecies similarity, respectively. Except for 5 isolates that presented biochemical profiles of Staphylococcus hominis subsp. hominis, but were identified as S. hominis subsp. novobiosepticus by SDS-PAGE, there was 100% accordance between the methods used. Comparison with the partial 16S-rDNA sequences confirmed by SDS-PAGE showed the high accuracy of this method to identify staphylococci subspecies and species.
Institute of Microbiology, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil.
Olinda Cabral
OC
da Silva Santos
Marinella Silva
MS
Laport
Lúcia Martins
LM
Teixeira
Marcia
M
Giambiagi-DeMarval
Natalia Lopes Pontes
NL
Iorio
Kátia Regina Netto
KR
dos Santos
D001426:Bacterial Proteins / Q000032:analysis* / Q000302:isolation & purification; D015373:Bacterial Typing Techniques / Q000379:methods; D016000:Cluster Analysis; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D006801:Humans; D020543:Proteome / Q000032:analysis* / Q000302:isolation & purification; D012680:Sensitivity and Specificity; D013203:Staphylococcal Infections / Q000382:microbiology*; D013210:Staphylococcus / Q000737:chemistry* / Q000145:classification* / Q000302:isolation & purification
D001426:Bacterial Proteins; D020543:Proteome