Study of efficacy of reactivator HI 6 in reactivation of immobilized acetylcholinesterase, inhibited by organophosphorus chemical warfare agents of the "G" series.
Reactivation with bis quaternary aldoxime HI-6, chemical formula 1-(2-hydroxyamino-methylpyridinium)-3-(4-carbamoylpyridinium)-2-oxapropane dichloride of immobilized enzyme acetylcholinesterase inhibited by nerve agent type "G" was studied. This aldoxime is effective in reactivation of sarin-inhibited acetylcholinesterase. Substantially lower reactivation potency was observed with cyclosarin-inhibited enzyme and almost no effect was found for that acetylcholinesterase is the enzyme complex. HI 6 is completely ineffective towards the soman-inhibited enzyme: After a 2-minute inhibition of the enzyme with soman no ability to define reactivator the inhibited enzymes and complexes.
University of Defence, Brno, Institute of NBC Defence, Víta Nejedlého, 682 03 Vyskov, Czech Republic. monika.hoskovcova@unob.cz
Monika
M
Hoskovcová
Emil
E
Halámek
Zbynĕk
Z
Kobliha
D000110:Acetylcholinesterase / Q000378:metabolism*; D000818:Animals; D015374:Biosensing Techniques; D001921:Brain / Q000201:enzymology; D002619:Chemical Warfare Agents / Q000494:pharmacology*; D002800:Cholinesterase Inhibitors / Q000494:pharmacology*; D002801:Cholinesterase Reactivators / Q000494:pharmacology*; D004800:Enzymes, Immobilized; D007202:Indicators and Reagents; D007700:Kinetics; D008956:Models, Chemical; D010091:Oximes; D011726:Pyridinium Compounds / Q000494:pharmacology*; D013552:Swine
D002619:Chemical Warfare Agents; D002800:Cholinesterase Inhibitors; D002801:Cholinesterase Reactivators; D004800:Enzymes, Immobilized; D007202:Indicators and Reagents; D010091:Oximes; D011726:Pyridinium Compounds; D000110:Acetylcholinesterase; C022870:asoxime chloride