Isolation and enrichment of stem cells.
Stem cells have the potential to revolutionize tissue regeneration and engineering. Both general types of stem cells, those with pluripotent differentiation potential as well as those with multipotent differentiation potential, are of equal interest. They are important tools to further understanding of general cellular processes, to refine industrial applications for drug target discovery and predictive toxicology, and to gain more insights into their potential for tissue regeneration. This chapter provides an overview of existing sorting technologies and protocols, outlines the phenotypic characteristics of a number of different stem cells, and summarizes their potential clinical applications.
Bosio
Andreas
A
Miltenyi Biotec GmbH, Friedrich-Ebert-Strasse 68, 51429, Bergisch Gladbach, Germany.
Huppert
Volker
V
Donath
Susan
S
Hennemann
Petra
P
Malchow
Michaela
M
Heinlein
Uwe A O
UA
D000328:Adult; D053687:Adult Stem Cells / Q000166:cytology* / Q000502:physiology; D002454:Cell Differentiation; D002478:Cells, Cultured; D053595:Embryonic Stem Cells / Q000166:cytology* / Q000502:physiology; D005434:Flow Cytometry; D048091:Guided Tissue Regeneration; D018380:Hematopoietic Stem Cell Transplantation; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000502:physiology; D006801:Humans; D018189:Immunomagnetic Separation / Q000379:methods*; D039902:Multipotent Stem Cells / Q000166:cytology* / Q000502:physiology; D023822:Tissue Engineering; D014184:Transplantation, Homologous