Assessment of cryopreserved hepatocytes as an alternative to fresh hepatocytes for comparative interspecies metabolism studies with suitable acceptance criteria.
Fresh hepatocytes have been the choice for interspecies comparative drug metabolism studies. Cryopreserved hepatocytes represent a readily available alternative when combined with acceptance limits based on the metabolic turnover of 7-ethoxycoumarin. Results for the ten NCEs examined show that the metabolites formed were strongly correlated in fresh and cryopreserved hepatocytes.
Cheng
Ziqiang Zack
ZZ
Department of World-wide Drug Metabolism and Pharmacokinetics, GlaxoSmithKline, Research Triangle Park, North Carolina 27709, USA. ziqiang.z.cheng@gsk.com
Herron
Christine E
CE
Bowers
Gary
G
de Serres
Mark
M
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000818:Animals; D003374:Coumarins / Q000378:metabolism*; D015925:Cryopreservation; D004285:Dogs; D005260:Female; D022781:Hepatocytes / Q000378:metabolism*; D006801:Humans; D008252:Macaca fascicularis; D008297:Male; D051379:Mice; D008875:Middle Aged; D004364:Pharmaceutical Preparations / Q000378:metabolism*; D011817:Rabbits; D051381:Rats; D013045:Species Specificity
D003374:Coumarins; D004364:Pharmaceutical Preparations; C017299:7-ethoxycoumarin