Removal of two organophosphate pesticides by a bacterial consortium immobilized in alginate or tezontle.
In order to remove methyl-parathion (MP) and tetrachlorvinphos (TCF), a bacterial consortium was immobilized with two supports consisting of alginate beads or stones of tezontle colonized by biofilm. Removal kinetics were recorded for suspended and immobilized consortium using a mineral salt medium supplemented with MP and TCF at 25mg/L and with 0.1% (w/v) glucose as a co-substrate. The viability of the consortium cultivated in suspension was maintained for 6 days, whereas the viability of the consortium immobilized in alginate and tezontle supports was maintained for up to 11 and 13 days, respectively. Growth was enhanced when using glucose as a co-substrate. The percentage of MP removed was significantly higher (alpha=0.05) when consortium was immobilized in alginate beads and biofilm on tezontle as compared to suspension culture.
Laboratorio de Investigaciones Ambientales, Centro de Investigación en Biotecnología, Universidad Autónoma del Estado de Morelos, avenida universidad 1001, colonia Chamilpa Cuernavaca, Morelos, CP 62209, Mexico.
Gustavo
G
Yañez-Ocampo
Enrique
E
Sanchez-Salinas
Gloria Alicia
GA
Jimenez-Tobon
Michel
M
Penninckx
María Laura
ML
Ortiz-Hernández
D000464:Alginates; D001419:Bacteria / Q000254:growth & development / Q000378:metabolism*; D020723:Glucuronic Acid; D006603:Hexuronic Acids; D008855:Microscopy, Electron, Scanning; D009943:Organophosphorus Compounds / Q000302:isolation & purification* / Q000378:metabolism; D010575:Pesticides / Q000302:isolation & purification* / Q000378:metabolism
D000464:Alginates; D006603:Hexuronic Acids; D009943:Organophosphorus Compounds; D010575:Pesticides; D020723:Glucuronic Acid