Probing in vivo origins of mononuclear phagocytes by conditional ablation and reconstitution.
Dendritic cells (DCs) and macrophages (MPhis), collectively termed mononuclear phagocytes (MP), are crucial for homeostatic tissue maintenance as well as the innate and adaptive host defense. These pleiotropic functions are likely to be covered by distinct DC and MPhi subsets, defined by anatomic location and molecular make-up. However, task division within the MP system remains poorly defined. A key to understanding of this issue, which might have important implications for the development of future therapeutic strategies, is the elucidation of the in vivo origins of DCs and MPhis, whose study recently gained striking momentum. Here we present methods to investigate the role of MP progenitors, such as monocytes and MPhi/DC precursors (MDPs), in the replenishment of the peripheral MP system.
Department of Immunology, The Weizmann Institute of Science, Rehovot, Israel.
Chen
C
Varol
Limor
L
Landsman
Steffen
S
Jung
D055011:Ablation Techniques / Q000379:methods*; D019264:Adoptive Transfer; D000818:Animals; D039521:CD11c Antigen / Q000378:metabolism; D066257:Heparin-binding EGF-like Growth Factor; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins / Q000378:metabolism; D007963:Leukocytes, Mononuclear / Q000166:cytology* / Q000637:transplantation; D051379:Mice; D008822:Mice, Transgenic; D009000:Monocytes / Q000166:cytology; D010586:Phagocytes / Q000166:cytology* / Q000637:transplantation
D039521:CD11c Antigen; C587883:HBEGF protein, human; C587884:Hbegf protein, mouse; D066257:Heparin-binding EGF-like Growth Factor; D036341:Intercellular Signaling Peptides and Proteins