Determination of cytochrome c in human serum and pharmaceutical injections using flow injection chemiluminescence.
It was found that the complex of cytochrome c (Cyt c) and hydrogen peroxide could significantly catalyze the chemiluminescence (CL) reaction from luminol-hydrogen peroxide, and a sensitive, rapid, and simple CL procedure was proposed for the determination of Cyt c in a flow injection system for the first time. The increment of CL intensity was linear over the concentration of Cyt c ranging from 5 to 700 ng ml(-1), with a detection limit of 2 ng ml(-1) (3sigma). At a flow rate of 2.0 ml min(-1), a complete analytical process could be performed in 30 s with a relative standard deviation of less than 4.0%. The proposed method was applied successfully for the assay of Cyt c in pharmaceutical injections and human serum, and the recoveries were from 98.0% to 108.8% and 92.5% to 109.0%. The possible mechanism of Cyt c enhanced CL reaction was also discussed.
Li
Xin
X
Key Laboratory of Synthetic and Natural Functional Molecule Chemistry of Ministry of Education, College of Chemistry & Material Science, Shaanxi Key Laboratory of Physico-Inorganic Chemistry, Northwest University, Xi'an 710069, China.
Liu
Houyong
H
He
Xili
X
Song
Zhenghua
Z
D045304:Cytochromes c / Q000097:blood*; D006801:Humans; D006861:Hydrogen Peroxide / Q000737:chemistry / Q000494:pharmacology; D008163:Luminescent Measurements / Q000379:methods*; D008165:Luminol / Q000737:chemistry; D004364:Pharmaceutical Preparations / Q000032:analysis
D004364:Pharmaceutical Preparations; D008165:Luminol; D045304:Cytochromes c; D006861:Hydrogen Peroxide