A high-throughput screening method to identify potential pesticides for mosquito control.
Mosquitoes that transmit human diseases are of major importance to the international public health community. Pesticides remain a major component of integrated programs to control these medically important species. However, very few types of pesticides are currently registered for mosquito control. A high-throughput screening method using first-instar larvae of Aedes aegypti was created and evaluated in our laboratory to quickly screen large numbers of chemicals for activity against mosquitoes. LC50 values of a representative group of compounds were determined using this high-throughput screening method and compared with LD50 values determined by topical application against female adults of Ae. aegypti. Our results show that this high-throughput screening method is suitable for screening large numbers of candidate chemicals quickly to identify effective compounds.
Center for Medical, Agricultural, and Veterinary Entomology, USDA-ARS, 1600 SW 23rd Dr., Gainesville, FL 32608, USA. julia.pridgeon@ars.usda.gov
Julia W
JW
Pridgeon
James J
JJ
Becnel
Gary G
GG
Clark
Kenneth J
KJ
Linthicum
D000330:Aedes; D000818:Animals; D007306:Insecticides / Q000032:analysis*; D007814:Larva; D007928:Lethal Dose 50; D009032:Mosquito Control; D018675:Toxicity Tests / Q000379:methods*
D007306:Insecticides