Flow cytometric analysis of T-lymphocytes from nasopharynx-associated lymphoid tissue (NALT) in a model of secondary immunodeficiency in Wistar rats.
Nasopharynx-associated lymphoid tissue (NALT) is responsible for immune responses in the upper respiratory tract of rodents. In our model of protein malnutrition (R21 group), bronchus-associated lymphoid tissue (BALT), situated in the lower respiratory tract, showed a decrease of CD4(+), CD8alpha(+), and TCRalphabeta(+) lymphocytes but TCRgammadelta(+) cells were increased. Besides, there is no information regarding the frequencies of T-cell populations in 60-day-old Wistar rats (C60 group). So, the aim of the present study was to analyze by flow cytometry NALT T-cells from both groups. NALT lymphocytes were isolated from R21 and C60 groups and stained with different antibodies. Samples were run on a FACScalibur flow cytometer. Background staining was evaluated using isotype controls. Data analysis was performed using BD Cell Quest and WinMDI 2.9. In C60, the predominant population was CD4(+)TCRalphabeta(+), which was significantly diminished in the R21 group. However, CD8alpha(+), the majority expressing CD8alphabeta, and TCRgammadelta(+) cells were not affected. In our model of secondary immunodeficiency, there is a compartmentalization between NALT and BALT because they differ in the populations affected even though they are inductive sites of the respiratory tract in the common mucosal immune system.
Sosa
Gustavo Adolfo
GA
Department of Biological Sciences, School of Pharmacy and Biochemistry, University of Buenos Aires, Buenos Aires, Argentina.
Quiroga
María Florencia
MF
Roux
María Estela
ME
D000818:Animals; D015704:CD4 Antigens / Q000276:immunology / Q000378:metabolism; D016827:CD8 Antigens / Q000276:immunology / Q000378:metabolism; D002469:Cell Separation; D004195:Disease Models, Animal; D005247:Feeding Behavior; D005434:Flow Cytometry; D018928:Immunity, Mucosal; D007153:Immunologic Deficiency Syndromes / Q000276:immunology* / Q000473:pathology; D008221:Lymphoid Tissue / Q000276:immunology* / Q000378:metabolism; D009297:Nasal Mucosa / Q000276:immunology; D009305:Nasopharynx / Q000276:immunology* / Q000378:metabolism; D011502:Protein-Energy Malnutrition / Q000276:immunology*; D051381:Rats; D017208:Rats, Wistar; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000276:immunology / Q000378:metabolism; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000276:immunology / Q000378:metabolism; D016176:T-Lymphocyte Subsets / Q000276:immunology / Q000378:metabolism*
D015704:CD4 Antigens; D016827:CD8 Antigens; D016693:Receptors, Antigen, T-Cell, alpha-beta; D016692:Receptors, Antigen, T-Cell, gamma-delta