Digital ion trap mass spectrometer for probing the structure of biological macromolecules by gas phase X-ray scattering.
Small-angle X-ray scattering is a technique for the characterization and structural analysis of a variety of materials including biological macromolecules and polymers. For the conformational analysis of proteins, the interaction between sample and X-rays is generally performed when the proteins are present in solution. Here a three-dimensional digital ion trap interfaced with a high intensity X-ray source is built to prove that X-ray scattering can be performed on ions isolated in gas-phase. Initial experiments on an unresolved ion population of multiply charged cytochrome C ions indicate that a small-angle X-ray scattering signal can be detected and that partial structural information can be extracted about the overall molecular structure of protein ions.
McCullough
Bryan J
BJ
Michael Barber Centre for Mass Spectrometry, Manchester Interdisciplinary Biocentre, The University of Manchester, Manchester, UK.
Entwistle
Andrew
A
Konishi
Ikuo
I
Buffey
Steven
S
Hasnain
S Samar
SS
Brancia
Francesco L
FL
Grossmann
J Günter
JG
Gaskell
Simon J
SJ
D001704:Biopolymers / Q000737:chemistry*; D045304:Cytochromes c / Q000737:chemistry; D004867:Equipment Design; D005740:Gases / Q000737:chemistry*; D013058:Mass Spectrometry / Q000295:instrumentation / Q000379:methods*; D010455:Peptides / Q000737:chemistry; D053838:Scattering, Small Angle; D014961:X-Ray Diffraction
D001704:Biopolymers; D005740:Gases; D010455:Peptides; D045304:Cytochromes c