Disruption of OsYSL15 leads to iron inefficiency in rice plants.
Uptake and translocation of metal nutrients are essential processes for plant growth. Graminaceous species release phytosiderophores that bind to Fe(3+); these complexes are then transported across the plasma membrane. We have characterized OsYSL15, one of the rice (Oryza sativa) YS1-like (YSL) genes that are strongly induced by iron (Fe) deficiency. The OsYSL15 promoter fusion to beta-glucuronidase showed that it was expressed in all root tissues when Fe was limited. In low-Fe leaves, the promoter became active in all tissues except epidermal cells. This activity was also detected in flowers and seeds. The OsYSL15:green fluorescent protein fusion was localized to the plasma membrane. OsYSL15 functionally complemented yeast strains defective in Fe uptake on media containing Fe(3+)-deoxymugineic acid and Fe(2+)-nicotianamine. Two insertional osysl15 mutants exhibited chlorotic phenotypes under Fe deficiency and had reduced Fe concentrations in their shoots, roots, and seeds. Nitric oxide treatment reversed this chlorosis under Fe-limiting conditions. Overexpression of OsYSL15 increased the Fe concentration in leaves and seeds from transgenic plants. Altogether, these results demonstrate roles for OsYSL15 in Fe uptake and distribution in rice plants.
Lee
Sichul
S
Department of Integrative Bioscience and Biotechnology, Pohang University of Science and Technology, Pohang 790-784, Republic of Korea.
Chiecko
Jeff C
JC
Kim
Sun A
SA
Walker
Elsbeth L
EL
Lee
Youngsook
Y
Guerinot
Mary Lou
ML
An
Gynheung
G
D000199:Actins / Q000235:genetics; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D017343:Genes, Plant; D005816:Genetic Complementation Test; D005966:Glucuronidase / Q000378:metabolism; D006706:Homeostasis / Q000187:drug effects / Q000235:genetics; D007501:Iron / Q000378:metabolism* / Q000494:pharmacology; D026901:Membrane Transport Proteins / Q000378:metabolism; D008969:Molecular Sequence Data; D009154:Mutation / Q000235:genetics; D009569:Nitric Oxide / Q000494:pharmacology; D012275:Oryza / Q000166:cytology / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D010641:Phenotype; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D018517:Plant Roots / Q000187:drug effects / Q000378:metabolism; D018520:Plant Shoots / Q000187:drug effects / Q000378:metabolism; D030821:Plants, Genetically Modified; D011401:Promoter Regions, Genetic / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012441:Saccharomyces cerevisiae / Q000166:cytology; D013347:Subcellular Fractions / Q000187:drug effects / Q000378:metabolism
D000199:Actins; D026901:Membrane Transport Proteins; D010940:Plant Proteins; D009569:Nitric Oxide; D007501:Iron; D005966:Glucuronidase