Strip immunoblotting of multiple antigenic peptides to nitrocellulose membrane.
Multiple antigenic peptides (MAPs) can be efficiently separated on sodium dodecyl sulfate (SDS) polyacrylamide gel and transferred to a nitrocellulose membrane for immunoblotting. MAPs involve a hepta lysine core with end groups for anchoring multiple copies of the same synthetic peptide. MAPs are amenable to staining with Coomassie and silver on SDS polyacrylamide gels as well as by Fast Green on a blotted nitrocellulose membrane. They lend themselves to analysis on an immunoblot as they behave like low molecular weight proteins. Affinity immunoblotting for analysis of antibody clonotype distribution has also been carried out using these peptides.
Oklahoma Medical Research Foundation, 825 NE 13th Street, OKC, OK, 73104, USA. biji-kurien@omrf.org
Biji T
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Kurien
D000595:Amino Acid Sequence; D000941:Antigens / Q000737:chemistry* / Q000276:immunology; D001324:Autoantigens / Q000737:chemistry / Q000235:genetics / Q000276:immunology; D003101:Collodion / Q000737:chemistry*; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D006801:Humans; D015151:Immunoblotting / Q000295:instrumentation / Q000379:methods; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010455:Peptides / Q000737:chemistry* / Q000276:immunology; D020733:RNA, Small Cytoplasmic / Q000737:chemistry / Q000235:genetics / Q000276:immunology; D012261:Ribonucleoproteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology; D000099207:SS-A Antigen
D000941:Antigens; D001324:Autoantigens; D003101:Collodion; D010455:Peptides; D020733:RNA, Small Cytoplasmic; D012261:Ribonucleoproteins; C116006:RO60 protein, human; D000099207:SS-A Antigen