Hydrogen production from formic acid in pH-stat fed-batch operation for direct supply to fuel cell.
Enterobacter asburiae SNU-1 harvested after cultivation was used as a whole cell biocatalyst, for the production of hydrogen. Formic acid was efficiently converted to hydrogen using the harvested cells with an initial hydrogen production rate and total hydrogen production of 491 ml/l/h and 6668 ml/l, respectively, when 1 g/l of whole cell enzyme was used. Moreover, new pH-stat fed-batch operation was conducted, and total hydrogen production was 1.4 times higher than that of batch operation. For practical application, bio-hydrogen produced from formic acid using harvested cells was directly applied to PEMFC for power generation.
School of Chemical and Biological Engineering, Seoul National University, Seoul, Republic of Korea.
Jong-Hwan
JH
Shin
Jong Hyun
JH
Yoon
Seung Hoon
SH
Lee
Tai Hyun
TH
Park
D001421:Bacteria, Anaerobic / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D055162:Biocatalysis; D019149:Bioreactors / Q000382:microbiology; D018929:Cell Culture Techniques / Q000379:methods*; D004305:Dose-Response Relationship, Drug; D004754:Enterobacter / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D005285:Fermentation; D005561:Formates / Q000378:metabolism* / Q000494:pharmacology; D006859:Hydrogen / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration / Q000187:drug effects; D013696:Temperature; D013997:Time Factors
D005561:Formates; C030544:formic acid; D006859:Hydrogen