DIXDC1 co-localizes and interacts with gamma-tubulin in HEK293 cells.
DIXDC1 is a Dishevelled-Axin (DIX) domain-containing protein involved in neural development and Wnt signaling pathway. Besides the DIX domain, DIXDC1 also contains a coiled-coil domain (MTH domain), which is a common feature of centrosomal proteins. We have demonstrated that exogenously expressed GFP-tag fused DIXDC1 co-localize with gamma-tubulin both at interphase and mitotic phase in HEK293 cells. By immunostaining with anti-DIXDC1 and anti-gamma-tubulin antibody, endogenous DIXDC1 was also co-localized with gamma-tubulin at the centrosomes in HEK293 cells. We confirmed this interaction of DIXDC1 with gamma-tubulin by co-immunoprecipitation. The findings suggest that DIXDC1 might play an important role in chromosome segregation and cell cycle regulation.
Wu
Yanrui
Y
Department of Brain Protection and Plasticity Research, Beijing Institute of Basic Medical Sciences, Beijing, PR China.
Jing
Xiaotang
X
Ma
Xin
X
Wu
Yan
Y
Ding
Xuefeng
X
Fan
Wenhong
W
Fan
Ming
M
D002460:Cell Line; D018385:Centrosome / Q000378:metabolism; D020090:Chromosome Segregation; D006801:Humans; D047468:Immunoprecipitation; D007399:Interphase; D047908:Intracellular Signaling Peptides and Proteins / Q000378:metabolism*; D008840:Microfilament Proteins / Q000378:metabolism*; D008938:Mitosis; D015398:Signal Transduction; D014404:Tubulin / Q000378:metabolism*
C513335:DIXDC1 protein, human; D047908:Intracellular Signaling Peptides and Proteins; D008840:Microfilament Proteins; D014404:Tubulin