[Expression profile of ADRP in macrophages and foam cells].
OBJECTIVE
To investigate the expression profile of adipose differentiation-related protein(ADRP)in macrophages and foam cells.


METHODS
THP-1 monocytes were differentiated into macrophages in vitro, and then induced into foam cells by culturing with oxidized low density lipoproteins(ox-LDLs). Real time RT-PCR and Western blot were performed to detect the expression of ADRP gene and protein.


RESULTS
The expression of ADRP both mRNA and protein were significantly increased when macrophages were further induced into foam cells by ox-LDLs, however, the same results were not observed during phorbol 12-myristate-13-acetate(PMA)-induced monocyte differentiation into macrophages.


CONCLUSIONS
Our findings revealed an upregulation of ADRP during macrophages differentiation into foam cells.
Department of Pathology and Pathophysiology, the Fourth Military Medical University, Xi'an 710032, China. liqing@fmmu.edu.cn
Yue
Y
Song
Hang
H
Li
Li-ying
LY
Zhang
Qing
Q
Ye
Qing
Q
Li
D015153:Blotting, Western; D002454:Cell Differentiation / Q000187:drug effects / Q000235:genetics; D002460:Cell Line; D005487:Foam Cells / Q000166:cytology / Q000378:metabolism*; D020869:Gene Expression Profiling; D006801:Humans; D008077:Lipoproteins, LDL / Q000494:pharmacology; D008264:Macrophages / Q000166:cytology / Q000378:metabolism*; D008565:Membrane Proteins / Q000235:genetics* / Q000378:metabolism; D009000:Monocytes / Q000166:cytology / Q000378:metabolism; D000070780:Perilipin-2; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D008077:Lipoproteins, LDL; D008565:Membrane Proteins; C000602982:PLIN2 protein, human; D000070780:Perilipin-2; C099252:oxidized low density lipoprotein; D013755:Tetradecanoylphorbol Acetate