The effects of nanoparticles uptaken by cells on electrorotation.
Electrorotation (ER) has become a very powerful diagnostic technique for the measurement of dielectric properties of cells. However, only a few papers have investigated the electric-induced rotation of particles in a stationary alternating (AC) electric field instead of a rotating electric field. In this study, a microchip composed of a top-grounded electrode, flow chamber and bottom chess-type electrode arrays was used to construct a stationary non-uniform AC electric field for the manipulation of cells by dielectrophoretic force. We focused on the effects of metal and dielectric nanoparticles uptaken by cells under ER, by using human promyelocytic leukemia cells (HL-60), 13 nm Au and 19 nm SiO(2) nanoparticles. As revealed by the experimental results, both the percentage of cells in rotation and the range of rotational (ROT) frequency for the uptake of Au nanoparticle cells were higher and wider than in the case of SiO(2) nanoparticles. In addition, the rotation of lone cells and pearl-chain cells under non-uniform and uniform electric field were quantitatively investigated, respectively. The membrane capacitance and membrane conductance of HL-60 cells can be extracted from the ROT spectra as 10.18+/-1.92 mF/m(2) and 1500+/-321 S/m(2), respectively. In general, the ER of cells in a stationary AC electric field can be attributed to the highly non-uniform electric field and non-uniform dispersion of nanoparticles within cells; therefore, the electrical properties of uptaken nanoparticles and the aggregation phenomenon have significant influences on the resulting electrical torque.
Chuang
Cheng-Hsin
CH
Department of Mechanical Engineering & Institute of Nanotechnology, Southern Taiwan University, Tainan, Taiwan. chchuang@mail.stut.edu.tw
Hsu
You-Ming
YM
Yeh
Chen-Che
CC
D002449:Cell Aggregation / Q000502:physiology; D002468:Cell Physiological Phenomena; D002470:Cell Survival / Q000502:physiology; D025802:Electric Capacitance; D004553:Electric Conductivity; D004574:Electromagnetic Fields; D004586:Electrophoresis / Q000295:instrumentation*; D004867:Equipment Design; D006046:Gold / Q000378:metabolism; D018922:HL-60 Cells; D006801:Humans; D053768:Metal Nanoparticles; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation; D012399:Rotation; D012822:Silicon Dioxide / Q000378:metabolism; D019415:Torque
D006046:Gold; D012822:Silicon Dioxide