Determination of talniflumate and niflumic acid in human plasma by liquid chromatography-tandem mass spectrometry.
A simple and rapid quantification method was developed for determining both talniflumate and niflumic acid in human plasma. After simple protein precipitation with acetonitrile, the analytes were chromatographed on a reversed-phase C(18) column and detected by LC/MS/MS with electrospray ionization. The assay accuracy and precision were within the FDA guidance for the analytical method validation. This method was used to measure the plasma concentrations of both compounds from healthy subjects after a single oral dose of talniflumate, 740 mg.
Kang
Wonku
W
College of Pharmacy, Catholic University of Daegu, Kyoungbuk 712-702, South Korea. wonkuk@cu.ac.kr
Kim
Kibum
K
D001572:Benzofurans / Q000008:administration & dosage / Q000097:blood* / Q000493:pharmacokinetics; D011232:Chemical Precipitation; D002853:Chromatography, Liquid; D006801:Humans; D016014:Linear Models; D009544:Niflumic Acid / Q000008:administration & dosage / Q000097:blood* / Q000493:pharmacokinetics; D011506:Proteins / Q000737:chemistry; D011725:Pyridines / Q000008:administration & dosage / Q000097:blood* / Q000493:pharmacokinetics; D012680:Sensitivity and Specificity; D012997:Solvents / Q000737:chemistry; D053719:Tandem Mass Spectrometry; D013997:Time Factors
D001572:Benzofurans; D011506:Proteins; D011725:Pyridines; D012997:Solvents; D009544:Niflumic Acid; C488233:talniflumate