Mitochondrial complex activity in donor renal grafts, cold ischemia time, and recovery of graft function.
Indexed mitochondrial complex activities (MCAi) were determined in biopsies obtained from 52 donor kidneys at the end of cold ischemia (8-32 hr) to see if longer anoxia affected MCAi and accounted for the increased risk of delayed graft function (DGF) in recipients of grafts with longer cold ischemia time (CIT) or from non-heart-beating donors (NHBD). CITs were significantly different between those with and without DGF (P=0.02), being shorter in the latter, but MCAi were similar. CIT was correlated (r=0.43, P=0.003) with the time taken for creatinine concentration to fall to half the perioperative value (Crt(1/2)) but not with MCAi. Frequency of DGF, greater in NHBD, was significantly different from that of heart-beating donors (P=0.04), but CIT and MCAi were similar. However, Crt(1/2), was significantly different being longer in NHBD. Thus, the frequency of DGF increased and the speed of recovery diminished with longer CIT, whereas MCAi remained stable suggesting other factors determined tissue recovery.
Department of Renal Transplantation, Queen Elizabeth Hospital, Birmingham, United Kingdom.
Chandrashekhar A
CA
Kubal
Robert M
RM
Harris
Nicholas G
NG
Inston
Keith P
KP
Graetz
Andrew R
AR
Ready
Stephen J
SJ
Mellor
Ahmed S
AS
Hamsho
Rosemary H
RH
Waring
Michael J
MJ
World
D000328:Adult; D001706:Biopsy; D002102:Cadaver; D002648:Child; D003576:Electron Transport Complex IV / Q000378:metabolism*; D005260:Female; D006085:Graft Survival / Q000502:physiology*; D006801:Humans; D007668:Kidney / Q000378:metabolism*; D016030:Kidney Transplantation / Q000473:pathology / Q000502:physiology*; D019520:Living Donors; D008875:Middle Aged; D008928:Mitochondria / Q000378:metabolism*; D010530:Peritoneal Dialysis / Q000706:statistics & numerical data; D044469:Racial Groups; D006435:Renal Dialysis / Q000706:statistics & numerical data; D014019:Tissue Donors
D003576:Electron Transport Complex IV