Single-molecule FRET analysis of protein-DNA complexes.
We present a single-molecule method for studying protein-DNA interactions based on fluorescence resonance energy transfer (FRET) and alternating-laser excitation (ALEX) of single diffusing molecules. An application of this method to the study of a bacterial transcription initiation complex is presented.
Heilemann
Mike
M
Department of Physics and IRC in Bionanotechnology, Clarendon Laboratory, University of Oxford, Parks Road, Oxford, OX1 3PU, UK.
Hwang
Ling Chin
LC
Lymperopoulos
Konstantinos
K
Kapanidis
Achillefs N
AN
D002021:Buffers; D002851:Chromatography, High Pressure Liquid; D004247:DNA / Q000302:isolation & purification / Q000378:metabolism*; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods*; D005456:Fluorescent Dyes / Q000378:metabolism; D007834:Lasers; D011506:Proteins / Q000378:metabolism*; D013056:Spectrophotometry, Ultraviolet; D013194:Staining and Labeling; D014158:Transcription, Genetic
D002021:Buffers; D005456:Fluorescent Dyes; D011506:Proteins; D004247:DNA; D012321:DNA-Directed RNA Polymerases