A flexizyme that selectively charges amino acids activated by a water-friendly leaving group.
We have developed a new flexizyme (a flexible de novo tRNA acylation ribozyme) system, a pair of amino-derivatized benzyl thioester (ABT) and amino flexizyme (aFx). ABT bearing the ammonium ion was designed to render the acyl-donor substrates better water solubility. Although the previously reported flexizymes (eFx and dFx) did not show acylation activity for the ABT derivatives, a new flexizyme variant aFx, generated by in vitro selection against an amino acid activated ABT, exhibits high selectivity toward those activated ABT. The flexizymes system including aFx, eFx, and dFx enables us to prepare a wide variety of acyl-tRNAs charged with non-proteinogenic amino acids.
Niwa
Nobuyoshi
N
Department of Chemistry and Biotechnology, Graduate School of Engineering, The University of Tokyo, Tokyo, Japan.
Yamagishi
Yusuke
Y
Murakami
Hiroshi
H
Suga
Hiroaki
H
D000596:Amino Acids / Q000737:chemistry*; D049148:Aminoacylation; D001483:Base Sequence; D016337:RNA, Catalytic / Q000737:chemistry / Q000378:metabolism*; D014867:Water / Q000737:chemistry*
D000596:Amino Acids; D016337:RNA, Catalytic; D014867:Water