[Preliminary study of p53 gene promoter binding site of activator protein-1 in A549 cell lines by chromatin immunoprecipitation].
OBJECTIVE
To detect and verify activator protein-1 (AP-1) binding to p53 gene promoter region in A549 cell lines in vitro.


METHODS
AP-1 binding to p53 gene promoter region was detected by chromatin immunoprecipitation (ChIP) assay using c-jun specific antibody, and PCR amplified its gene specific DNA fragment.


RESULTS
The p53 gene specific fragment was found in the DNA fragment immunoprecipitated by c-jun antibody.


CONCLUSIONS
AP-1 binds to p53 gene promoter region in A549 cells, and regulates its expression.
Shen
Fei
F
Department of Occupational Health and Environmental Health, Public Health College, Zhengzhou University, Zhengzhou, Henan Province 450001, China.
Li
Zhi-tao
ZT
Wu
Yi-ming
YM
Wu
Wei-dong
WD
D045744:Cell Line, Tumor; D047369:Chromatin Immunoprecipitation; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic / Q000235:genetics*; D011485:Protein Binding; D018808:Transcription Factor AP-1 / Q000235:genetics*; D016159:Tumor Suppressor Protein p53 / Q000235:genetics*
C495901:TP53 protein, human; D018808:Transcription Factor AP-1; D016159:Tumor Suppressor Protein p53