Interstitial concentrations of adipokines in subcutaneous abdominal and femoral adipose tissue.
Adipokines play important regulatory roles in the pathophysiology of obesity and insulin resistance. We measured plasma and interstitial concentrations of the adipokines adiponectin, resistin, leptin, monocyte chemoattractant protein-1 (MCP-1), interleukin-6 (IL-6) and interleukin-8 (IL-8) in subcutaneous, abdominal and femoral adipose tissue using calibrated, large-pore microdialysis technique in 8 healthy, lean men on 2 experimental days. The interstitial leptin concentration was 2.5-fold higher in subcutaneous, femoral than abdominal adipose tissue (P<0.05), but no regional differences were found for the remaining adipokines (P>0.05). Adiponectin and leptin concentrations were higher in plasma than subcutaneous adipose tissue (approximately 25-fold and approximately 2-fold, respectively, P<0.05), whereas MCP-1, IL-6 and IL-8 concentrations were higher in subcutaneous adipose tissue than plasma (approximately 100-fold, approximately 200-fold and approximately 1000-fold, respectively, P<0.05). Resistin concentrations did not differ significantly between compartments. Adipose tissue blood flow (ATBF) showed no regional difference (P>0.05). The intra- and inter-subject variations of all investigated adipokines as well as of ATBF were substantial (coefficient of variation: 4-177%). In conclusion, interstitial leptin concentrations are approximately 2.5-fold higher in subcutaneous, femoral than abdominal adipose tissue, which might be a potential mechanism behind the health-benefits of "pear-shape". Furthermore, subcutaneous adipose tissue has a marked production of pro-inflammatory adipokines.
Department of Biomedical Sciences, University of Copenhagen, DK-2200 Copenhagen N, Denmark.
Ninna Bo
NB
Nielsen
Lise
L
Højbjerre
Mette P
MP
Sonne
Amra C
AC
Alibegovic
Allan
A
Vaag
Flemming
F
Dela
Bente
B
Stallknecht
D050153:Abdominal Fat / Q000098:blood supply / Q000378:metabolism*; D054392:Adipokines / Q000378:metabolism*; D052242:Adiponectin / Q000378:metabolism; D000328:Adult; D018932:Chemokine CCL2 / Q000378:metabolism; D005269:Femur / Q000378:metabolism*; D006801:Humans; D015850:Interleukin-6 / Q000378:metabolism; D016209:Interleukin-8 / Q000378:metabolism; D020738:Leptin / Q000378:metabolism; D008297:Male; D017551:Microdialysis; D052243:Resistin / Q000378:metabolism; D050151:Subcutaneous Fat / Q000098:blood supply / Q000378:metabolism*; D055815:Young Adult
D054392:Adipokines; D052242:Adiponectin; D018932:Chemokine CCL2; D015850:Interleukin-6; D016209:Interleukin-8; D020738:Leptin; D052243:Resistin