Improved protein detection using cold microwave technology.
Protein screening/detection is an essential tool in many laboratories. Owing to the relatively large time investments that are required by standard protocols, the development of methods with higher throughput while maintaining an at least comparable signal-to-noise ratio would be highly beneficial to many researchers. This chapter describes how cold microwave technology can be used to enhance the rate of molecular interactions and provides protocols for dot blots, western blots, and ELISA procedures permitting a completion of all incubation steps (blocking and antibody steps) within 45 min.
Smith
Aaron G
AG
Microscopy and Imaging Center, Department of Biology and Department of Biochemistry and Biophysics, BSBW 119, Texas A&M University, College Station, TX, 77843-2257, USA.
Jayaram
Jyothi
J
Johnson
Carol B
CB
Ellis
E Ann
EA
Vitha
Stanislav
S
Collisson
Ellen W
EW
Holzenburg
Andreas
A
D000818:Animals; D015153:Blotting, Western / Q000379:methods*; D002478:Cells, Cultured; D003080:Cold Temperature; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D008872:Microwaves; D011506:Proteins / Q000032:analysis*; D013194:Staining and Labeling / Q000379:methods*
D011506:Proteins