Testing biological activity of model Maillard reaction products: studies on gastric smooth muscle tissues.
Water-soluble Maillard reaction products obtained from five different model systems were investigated for their effects upon the mechanical activity of rat gastric smooth muscle. Most of the total Maillard reaction products applied at concentration of 1.5 mg/ml evoked contractions; among them the product obtained from arginine and glucose (Arg-Glc) produced the most powerful contractions. The product obtained from glycine and ascorbic acid (Gly-AsA) was the only one that brought about relaxation response. The high molecular weight fractions (>3,500 Da) isolated from the reaction systems Arg-Glc and Gly-AsA demonstrated effects similar in type and amplitude to those evoked by non-fractioned reaction products. The results obtained suggest that moieties of molecules acting upon the muscle tonus originate mainly from lysine and arginine residues; that these structures are available in both low and high molecular pools in similar concentrations, and most likely these fragments act upon membrane-located cellular structures involved in calcium transport.
Department of Chemistry and Biochemistry, Medical University, 15a Vassil Aprilov Street, 4002 Plovdiv, Bulgaria. margirova@hotmail.com
Mariana D
MD
Argirova
Iliyana D
ID
Stefanova
Athanas D
AD
Krustev
Valentin I
VI
Turiiski
D000596:Amino Acids / Q000737:chemistry* / Q000494:pharmacology*; D000818:Animals; D001120:Arginine / Q000031:analogs & derivatives / Q000737:chemistry / Q000494:pharmacology; D001205:Ascorbic Acid / Q000031:analogs & derivatives / Q000737:chemistry / Q000494:pharmacology; D002241:Carbohydrates / Q000737:chemistry* / Q000494:pharmacology*; D005947:Glucose / Q000031:analogs & derivatives / Q000737:chemistry / Q000494:pharmacology; D005998:Glycine / Q000031:analogs & derivatives / Q000737:chemistry / Q000494:pharmacology; D066298:In Vitro Techniques; D008239:Lysine / Q000031:analogs & derivatives / Q000737:chemistry / Q000494:pharmacology; D015416:Maillard Reaction; D008297:Male; D008970:Molecular Weight; D009119:Muscle Contraction / Q000187:drug effects; D009129:Muscle Tonus / Q000187:drug effects; D009130:Muscle, Smooth / Q000187:drug effects* / Q000502:physiology; D011108:Polymers / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D012995:Solubility; D017550:Spectroscopy, Fourier Transform Infrared; D013270:Stomach; D014994:Xylose / Q000031:analogs & derivatives / Q000737:chemistry / Q000494:pharmacology
D000596:Amino Acids; D002241:Carbohydrates; D011108:Polymers; C011908:melanoidin polymers; D001120:Arginine; D014994:Xylose; D005947:Glucose; D008239:Lysine; D001205:Ascorbic Acid; D005998:Glycine