Gastric carcinogenesis and Helicobacter pylori infection.
Helicobacter pylori is the most frequent cause of infection-induced cancer worldwide. Gastric carcino-genesis is the consequence of the important and life-long inflammation induced by H. pylori in the stomach. Gastric carcinogenesis, can be studied in many ways. In this chapter, we focus on some aspects concerning the bacteria, and others concerning the host. On the bacterial side, the methods exploring the presence of the cag pathogenicity island including cagA and the consequences on epithelial cells are presented. On the host side, tissue microarray, immunohistochemistry and chromogenic in situ hybridization (CISH) are described.
INSERM U853, Université Victor Segalen, Bordeaux, France.
Christine
C
Varon
Jean-François
JF
Mosnier
Philippe
P
Lehours
Tamara
T
Matysiak-Budnik
Francis
F
Mégraud
D000595:Amino Acid Sequence; D000942:Antigens, Bacterial / Q000235:genetics; D001426:Bacterial Proteins / Q000235:genetics; D015373:Bacterial Typing Techniques; D001483:Base Sequence; D018929:Cell Culture Techniques; D002478:Cells, Cultured; D018920:Coculture Techniques; D004847:Epithelial Cells / Q000166:cytology / Q000382:microbiology; D005753:Gastric Mucosa / Q000166:cytology / Q000382:microbiology; D011905:Genes, ras; D044404:Genomic Islands / Q000235:genetics; D016481:Helicobacter Infections / Q000276:immunology* / Q000473:pathology; D016480:Helicobacter pylori / Q000235:genetics / Q000276:immunology / Q000472:pathogenicity*; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D016209:Interleukin-8 / Q000378:metabolism; D008969:Molecular Sequence Data; D011518:Proto-Oncogene Proteins / Q000235:genetics; D016283:Proto-Oncogene Proteins p21(ras); D016415:Sequence Alignment; D013274:Stomach Neoplasms / Q000209:etiology / Q000276:immunology* / Q000382:microbiology* / Q000473:pathology; D046888:Tissue Array Analysis / Q000379:methods; D018631:ras Proteins / Q000235:genetics
D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D016209:Interleukin-8; C117307:KRAS protein, human; D011518:Proto-Oncogene Proteins; C074689:VacA protein, Helicobacter pylori; C080866:cagA protein, Helicobacter pylori; D016283:Proto-Oncogene Proteins p21(ras); D018631:ras Proteins