Quantum dot-ruthenium complex dyads: recognition of double-strand DNA through dual-color fluorescence detection.
We have developed a new fluorescent ensemble probe comprising an ionic conjugate between water-soluble thioglycolic acid (TGA) capped CdTe quantum dots (QDs) and Ru(bpy)(2)(dppx)(2+) for the dual-color detection of complementary double-stranded DNAs (dsDNA). To provide the platform for DNA detection, the Ru-complex was first employed as an effective fluorescence quencher to TGA capped QDs via photoinduced electron transfer process. Because of its strong binding affinity with Ru(bpy)(2)(dppx)(2+), complementary dsDNA can break up the low fluoresced ionic ensemble, set free the luminescent QDs, and concomitantly generate the Ru(bpy)(2)(dppx)(2+) intercalated DNA complex. Thus, the recognition of dsDNA by Ru(bpy)(2)(dppx)(2+) can be realized via both the restoration of QDs fluorescence and the emergence of a new fluorescence emission signal of the quencher-substrate at 609 nm, while single-stranded DNA, ribonucleic acid, bovine albumin serum, and biological relevant metal ions cannot produce the similar results. Therefore, a simple, fast, sensitive, and highly selective assay for dsDNA has been realized.
Zhao
Dan
D
Department of Chemistry, Hong Kong Baptist University, Kowloon Tong, Hong Kong SAR, PR China.
Chan
W H
WH
He
Zhike
Z
Qiu
Ting
T
D000818:Animals; D015374:Biosensing Techniques; D002417:Cattle; D003116:Color; D004247:DNA / Q000032:analysis* / Q000737:chemistry*; D004579:Electron Transport; D005453:Fluorescence; D008163:Luminescent Measurements / Q000379:methods*; D055668:Photochemical Processes; D045663:Quantum Dots; D012428:Ruthenium / Q000737:chemistry*; D055672:Static Electricity; D013499:Surface Properties
D012428:Ruthenium; D004247:DNA; C427203:calf thymus DNA