A fractionation procedure for identifying novel proteins induced by chill stress in Arabidopsis thaliana.
Extraction of plant proteins using typical extraction buffers leaves insoluble debris that cannot be investigated by conventional 2-DE technologies. In this paper, we present a scalable, off-line procedure for extraction of Arabidopsis thaliana homogenates that can be used in combination with both in-gel digestion and mass spectrometry. Based on sequential NaCl gradients and strong detergent fractionation, this new strategy allowed detection of 11 novel proteins from Arabidopsis thaliana that were altered in response to chilling stress.
Key Lab for Molecular Enzymology and Engineering of the Ministry of Education, Jilin University, Changchun, 130023, People's Republic of China.
Liwen
L
Sun
Shichen
S
Wang
Jinghui
J
Xi
Shuo
S
Yang
Xiangguo
X
Liu
Xin
X
Chai
Hongbao
H
Xin
Baiyi
B
An
Dongyun
D
Hao
D017360:Arabidopsis / Q000737:chemistry* / Q000502:physiology*; D029681:Arabidopsis Proteins / Q000096:biosynthesis* / Q000302:isolation & purification*; D005591:Chemical Fractionation / Q000379:methods*; D003080:Cold Temperature; D003902:Detergents / Q000378:metabolism; D012965:Sodium Chloride / Q000378:metabolism; D013312:Stress, Physiological
D029681:Arabidopsis Proteins; D003902:Detergents; D012965:Sodium Chloride