Single molecule-sensitive probes for imaging RNA in live cells.
To visualize native or non-engineered RNA in live cells with single-molecule sensitivity, we developed multiply labeled tetravalent RNA imaging probes (MTRIPs). When delivered with streptolysin O into living human epithelial cancer cells and primary chicken fibroblasts, MTRIPs allowed the accurate imaging of native mRNAs and a non-engineered viral RNA, of RNA co-localization with known RNA-binding proteins, and of RNA dynamics and interactions with stress granules.
Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, Georgia, USA. philip.santangelo@bme.gatech.edu
Philip J
PJ
Santangelo
Aaron W
AW
Lifland
Paul
P
Curt
Yukio
Y
Sasaki
Gary J
GJ
Bassell
Michael E
ME
Lindquist
James E
JE
Crowe
D051377:Actin-Related Protein 2 / Q000235:genetics; D000199:Actins / Q000235:genetics; D000818:Animals; D030161:Avian Proteins / Q000235:genetics; D001426:Bacterial Proteins / Q000494:pharmacology; D001710:Biotin / Q000737:chemistry; D002232:Carbocyanines / Q000737:chemistry; D045744:Cell Line, Tumor; D002463:Cell Membrane Permeability / Q000187:drug effects; D002470:Cell Survival; D002478:Cells, Cultured; D002642:Chick Embryo; D004268:DNA-Binding Proteins / Q000235:genetics; D005347:Fibroblasts / Q000378:metabolism; D005456:Fluorescent Dyes / Q000032:analysis; D006801:Humans; D007091:Image Processing, Computer-Assisted; D018613:Microscopy, Confocal; D008856:Microscopy, Fluorescence; D015336:Molecular Probe Techniques; D015345:Oligonucleotide Probes / Q000032:analysis / Q000737:chemistry; D039102:Poly(A)-Binding Proteins / Q000378:metabolism; D012313:RNA / Q000032:analysis* / Q000378:metabolism; D012333:RNA, Messenger / Q000032:analysis / Q000378:metabolism; D016601:RNA-Binding Proteins / Q000235:genetics; D018113:Respiratory Syncytial Virus, Human / Q000235:genetics; D019809:Streptavidin / Q000737:chemistry; D013301:Streptolysins / Q000494:pharmacology; D000074019:T-Cell Intracellular Antigen-1
D051377:Actin-Related Protein 2; D000199:Actins; D030161:Avian Proteins; D001426:Bacterial Proteins; D002232:Carbocyanines; D004268:DNA-Binding Proteins; D005456:Fluorescent Dyes; D015345:Oligonucleotide Probes; D039102:Poly(A)-Binding Proteins; D012333:RNA, Messenger; D016601:RNA-Binding Proteins; D013301:Streptolysins; D000074019:T-Cell Intracellular Antigen-1; C071558:TIA1 protein, human; C498636:ZBP1 protein, Gallus gallus; C485731:ZBP1 protein, human; C089187:cyanine dye 3; C025964:streptolysin O; D012313:RNA; D001710:Biotin; D019809:Streptavidin