Development of an in vitro photosensitization assay using human monocyte-derived cells.
In this study, with the aim of developing a cell-based in vitro photosensitization assay, we examined whether changes of CD86 and CD54 expression on cells of a human monocytic cell line, THP-1, could be used to assess the photosensitizing potential of chemicals. First, we identified suitable conditions of UV-irradiation (irradiation dose; 5.0 J/cm(2), irradiation intensity; 1.7 mW/cm(2)) by investigating the effect of UV-irradiation on CD86 and CD54 expression on untreated or 6-methylcoumarin (a representative photoallergen)-treated THP-1 cells (irradiation method). However, acridine, a representative photo-irritant, augmented CD86 and CD54 expression on THP-1 cells, apparently via induction of reactive oxygen species (ROS). In order to abolish the effect of ROS, we examined CD86 and CD54 expression on THP-1 cells treated with pre-irradiated chemicals (pre-irradiation method). We found that UV-irradiated photoallergens, but not photo-irritants, enhanced CD86 and/or CD54 expression on the THP-1 cells. Finally, based on the results of irradiation, non-irradiation, and pre-irradiation with 18 test chemicals, we built a decision tree, which allows us to distinguish between photoallergens and photo-irritants. We suggest that this system may be useful for in vitro evaluation of the photoallergic potential of chemicals.
Hoya
Masatoshi
M
Shiseido Quality Assessment Center, Shiseido Co Ltd, Yokohama-shi, Kanagawa, Japan. masatoshi.hoya@to.shiseido.co.jp
Hirota
Morihiko
M
Suzuki
Mie
M
Hagino
Shigenobu
S
Itagaki
Hiroshi
H
Aiba
Setsuya
S
D000166:Acridines / Q000633:toxicity; D000485:Allergens / Q000633:toxicity; D051940:B7-2 Antigen / Q000235:genetics / Q000528:radiation effects; D002460:Cell Line; D003374:Coumarins / Q000633:toxicity; D003663:Decision Trees; D004307:Dose-Response Relationship, Radiation; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000235:genetics / Q000528:radiation effects; D007509:Irritants / Q000633:toxicity; D009000:Monocytes / Q000187:drug effects* / Q000528:radiation effects; D017319:Photosensitizing Agents / Q000633:toxicity*; D017382:Reactive Oxygen Species / Q000378:metabolism; D018675:Toxicity Tests / Q000379:methods*; D014466:Ultraviolet Rays
D000166:Acridines; D000485:Allergens; D051940:B7-2 Antigen; D003374:Coumarins; D007509:Irritants; D017319:Photosensitizing Agents; D017382:Reactive Oxygen Species; D018799:Intercellular Adhesion Molecule-1; C018096:6-methylcoumarin