Identification of PKC isoforms expressed in human bronchial smooth muscle cell.
The PKC family has been implicated in a wide range of biological response in a number of different cellular systems. Although the functions of PKC have been vigorously studied using pharmacological tools, there is little information about the expression pattern of its isoforms in human bronchial smooth muscle cells (hBSMCs). In the present study, RT-PCR and immunoblot analyses were carried out to detect all the PKC isoforms expressed in cultured normal hBSMCs. The RT-PCR analyses revealed that mRNAs of the known PKC isoforms except for PKCgamma and theta were detected in the hBSMCs. Among them, protein expressions of the isoforms except for PKCeta and mu were also detected by immunoblottings. Thus, the present study clearly demonstrated the expression of multiple PKC isoforms, i.e., PKCalpha, beta, delta, epsilon, zeta and iota in hBSMCs.
Sakai
Hiroyasu
H
Department of Pharmacology, School of Pharmacy, Hoshi University, 2-4-41 Ebara, Shinagawa-ku, Tokyo, Japan. sakai@hoshi.ac.jp
Yamamoto
Maki
M
Kozutsumi
Yuri
Y
Chiba
Yoshihiko
Y
Misawa
Miwa
M
D001980:Bronchi / Q000166:cytology / Q000201:enzymology*; D002478:Cells, Cultured; D015971:Gene Expression Regulation, Enzymologic / Q000502:physiology*; D006801:Humans; D007527:Isoenzymes / Q000096:biosynthesis; D032389:Myocytes, Smooth Muscle / Q000166:cytology / Q000201:enzymology*; D009928:Organ Specificity / Q000502:physiology; D011493:Protein Kinase C / Q000096:biosynthesis*
D007527:Isoenzymes; D011493:Protein Kinase C