Inhibitory Effect of Codeine on Sucrase Activity
Codeine is a common drug widely used in some countries as a pain reliever. The effect of codeine on yeast sucrase activity was studied in this report. Non-competitive inhibition was observed using double reciprocal plot. The K(m) of enzyme did not change in the presence of different concentrations of codeine (0.5- 1.5 mM) and was determined about 11.5 mM. The V(max) of enzyme was determined about 8.8 mM/min, and the V(max) decreased in the presence of codeine. The K(i) of codeine was measured by using the reaction rate and the initial concentration of the inhibitor according to the Dixon plot. The K(i) was found to be 0.42 mM and the IC(50) of codeine was determined about 0.875 mM.
Minai-Tehrani
Dariush
D
BioResearch Lab, Faculty of Biological Sciences, Shahid Beheshti University, G.C, Tehran, Iran
Minoui
Saeed
S
Environmental Sciences Research Institute, Shahid Beheshti University, G.C, Tehran, Iran
Sepehre
Marzie
M
BioResearch Lab, Faculty of Biological Sciences, Shahid Beheshti University, G.C, Tehran, Iran
Sharifkhodaei
Zohreh
Z
BioResearch Lab, Faculty of Biological Sciences, Shahid Beheshti University, G.C, Tehran, Iran
AavaniSucrase,
Tooka
T
BioResearch Lab, Faculty of Biological Sciences, Shahid Beheshti University, G.C, Tehran, Iran
D000701:Analgesics, Opioid / Q000494:pharmacology*; D003061:Codeine / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004791:Enzyme Inhibitors; D006868:Hydrolysis; D007700:Kinetics; D013053:Spectrophotometry; D013393:Sucrase / Q000037:antagonists & inhibitors*; D013395:Sucrose / Q000378:metabolism
D000701:Analgesics, Opioid; D004791:Enzyme Inhibitors; D013395:Sucrose; D013393:Sucrase; D003061:Codeine