Human papillomaviruses are identified in a subgroup of sinonasal squamous cell carcinomas with favorable outcome.
BACKGROUND
The role of human papillomavirus (HPV) in the pathogenesis of squamous cell carcinomas (SCCs) of the sinonasal tract and its clinicopathological implications were evaluated.


METHODS
All SCCs of the sinonasal tract diagnosed in the Hospital Clinic of Barcelona from 1981 to 2006 were retrospectively evaluated (N = 60). Clinical and pathological data were reviewed. HPV infection was determined and typed by amplification of HPV DNA by polymerase chain reaction using the SPF-10 primers. p16(INK4a) expression was determined by immunohistochemistry. Overall and progression-free survival for HPV-positive and -negative patients was estimated by Kaplan-Meier analysis and by the use of a multivariate Cox proportional hazards model.


RESULTS
HPV DNA was detected in tumor tissue of 12 of 60 (20%) patients. HPV16 was identified in 11 tumors and HPV35 in 1. Immunohistochemistry for p16(INK4a) stained all HPV-positive and no HPV-negative tumors (P < .001). No differences were observed in terms of site and histological grade or stage at presentation between HPV-positive and -negative tumors. However, HPV-positive patients had a significantly better 5-year progression-free survival (62%; 95% confidence interval [CI], 23%-86% vs 20%; 95% CI, 9%-34%; P = .0043, log-rank test) and overall survival (80%; 95% CI, 20%-96% vs 31%; 95% CI, 15%-47%; P = .036, log-rank test) than patients with HPV-negative tumors. In multivariate analysis, HPV-positive tumors were associated with improved progression-free survival (hazard ratio, 0.21; 95% CI, 0.17-0.98; P = .012).


CONCLUSIONS
A subgroup of sinonasal SCCs is associated with HPV infection. These tumors have a significantly better prognosis.
Alos
Llucia
L
Department of Pathology, Hospital Clinic, IDIBAPS, University of Barcelona, Barcelona, Spain. lalos@clinic.ub.es
Moyano
Susana
S
Nadal
Alfons
A
Alobid
Isam
I
Blanch
Jose L
JL
Ayala
Edgar
E
Lloveras
Belén
B
Quint
Wim
W
Cardesa
Antonio
A
Ordi
Jaume
J
D000368:Aged; D000369:Aged, 80 and over; D002294:Carcinoma, Squamous Cell / Q000473:pathology / Q000821:virology*; D004279:DNA, Viral / Q000032:analysis; D018572:Disease-Free Survival; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D027383:Papillomaviridae / Q000302:isolation & purification*; D030361:Papillomavirus Infections / Q000150:complications; D010255:Paranasal Sinus Neoplasms / Q000473:pathology / Q000821:virology*; D011379:Prognosis; D016019:Survival Analysis
D004279:DNA, Viral