Gene expression of human beta-defensins in healthy and inflamed human dental pulps.
Human beta-defensins (hBDs) are antimicrobial peptides that play an important role in the innate host defense against bacterial invasion, contribute to promotion of adaptive immune responses, and show chemotactic activities. The aim of this study was to compare the gene expression of hBD-1, -2, -3, and -4 in healthy teeth and teeth with pulpitis. Samples of healthy and inflamed dental pulps were obtained from extracted third molars and during treatment of teeth with pulpitis. Gene expression was assessed by using reverse transcriptase reaction and real-time polymerase chain reaction. HBD-2 and hBD -3 were only weakly expressed in healthy and inflamed pulps. In contrast, the expression of hBD-1 and hBD -4 was significantly increased in inflamed compared with healthy pulps. These results suggest that hBD-1 and hBD-4 might play a role in the pulpal host defense.
Department of Operative Dentistry and Periodontology, University School of Dental Medicine, Charité-Universitätsmedizin Berlin, Berlin, Germany. paris@konspar.uni-kiel.de
Sebastian
S
Paris
Michael
M
Wolgin
Andrej Michael
AM
Kielbassa
Axel
A
Pries
Andreas
A
Zakrzewicz
D003782:Dental Pulp / Q000276:immunology / Q000378:metabolism*; D015870:Gene Expression; D006801:Humans; D018928:Immunity, Mucosal / Q000502:physiology; D011671:Pulpitis / Q000276:immunology / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015854:Up-Regulation; D023083:beta-Defensins / Q000096:biosynthesis* / Q000235:genetics
D023083:beta-Defensins