[A recombination event occurring between HLA-B and DRB1 loci within a Chinese Han family].
OBJECTIVE
To investigate a recombination event occurring between the HLA-B and DRB1 loci in a Chinese family with a leukemia patient.


METHODS
HLA class I (-A and -B) low resolution typing was carried out by polymerase chain reaction-sequence specific oligonucleotide, PCR-SSO). HLA class II low resolution typing was performed by PCR-sequence specific primer (PCR-SSP). And HLA class I and II high resolution typing was done by sequencing-based typing (SBT). Then the recombination event was analyzed by family study.


RESULTS
The 2 haplotypes of the patient were A*3101-B*1301-DRB1*0701 and A*3303-B*4403-DRB1*1302. His father's 2 haplotypes were A*3001-B*1302-DRB1*0701 and A*3101-B*1301-DRB1*1501. Family study demonstrated that the HLA-A*3101-B*1301 was from one of his father's chromosome and the DRB1*0701 was from the other chromosome of his father. So the result indicated that the recombination event occurred between the HLA-B and -DRB1 loci during meiosis of his father and resulted in a new HLA haplotype that was transferred to the son.


CONCLUSIONS
A HLA-B/DR recombination event occurring between the HLA-B and -DRB1 loci has been found in a Chinese family, which may help further study of the mechanism of HLA recombination.
HLA Laboratory, Beijing Red Cross Blood Center, Beijing, People's Republic of China.
Na
N
Liu
Xiao-yan
XY
Shan
Wei
W
Li
Lijun
L
Wang
Xiao-mei
XM
He
Dong-mei
DM
Wang
Lei
L
Ni
Shuang
S
Cui
Lin
L
Wang
Zhi-yin
ZY
Gong
Bo-tao
BT
Zhao
Zhi-xin
ZX
Zhang
D000293:Adolescent; D044466:Asian People / Q000235:genetics*; D003434:Crossing Over, Genetic / Q000235:genetics; D005190:Family; D005260:Female; D015234:HLA-A Antigens / Q000235:genetics*; D015235:HLA-B Antigens / Q000235:genetics*; D006683:HLA-DQ Antigens / Q000235:genetics*; D059866:HLA-DQ beta-Chains; D006684:HLA-DR Antigens / Q000235:genetics*; D059811:HLA-DRB1 Chains; D006801:Humans; D008297:Male; D010375:Pedigree; D016133:Polymerase Chain Reaction; D011995:Recombination, Genetic
D015234:HLA-A Antigens; D015235:HLA-B Antigens; D006683:HLA-DQ Antigens; D059866:HLA-DQ beta-Chains; C069051:HLA-DQB1 antigen; D006684:HLA-DR Antigens; D059811:HLA-DRB1 Chains