Measurement of human serum IgD levels.
This unit describes an ELISA for the quantitative measurement of IgD levels in human serum. The ELISA is highly specific and sensitive, with a minimum detectable concentration of 30 pg/ml and more than 10,000-fold specificity for IgD over all other human immunoglobulins. Linear dilution characteristics enable measurement of IgD concentrations ranging over 5 orders of magnitude. These factors are vital for the IgD assay, since IgD makes up only a small proportion of the total immunoglobulins present in normal sera, and IgD serum concentrations are known to vary widely between individuals.
Department of Medicine, Addenbrooke's Hospital, Cambridge, United Kingdom.
Catherine L
CL
Overed-Sayer
Pronostics Ltd., Babraham, Cambridge, United Kingdom.
David E
DE
Mosedale
Division of Immunity and Infection, The Medical School, University of Birmingham, Birmingham, United Kingdom.
Margaret
M
Goodall
Department of Medicine, Addenbrooke's Hospital, Cambridge, United Kingdom.
David J
DJ
Grainger
D004797:Enzyme-Linked Immunosorbent Assay / Q000295:instrumentation / Q000379:methods*; D006801:Humans; D007072:Immunoglobulin D / Q000097:blood*; D054078:Mevalonate Kinase Deficiency / Q000097:blood / Q000276:immunology; D012680:Sensitivity and Specificity
D007072:Immunoglobulin D