Testosterone might cause relaxation of human corpus cavernosum by potassium channel opening action.
OBJECTIVE
To investigate the effect of testosterone on contractile tone of endothelium-denuded human corpus cavernosum strips. Human studies designed to examine a possible relaxant effect of testosterone on corpus cavernosal circulation are lacking.


METHODS
Testosterone (0.1-300 microM) was added cumulatively to organ baths after precontraction of isolated human corpus cavernosum strips (n = 5) with KCl (45 mM). Testosterone-induced responses were tested in the presence of nonselective, large, conductance Ca(2+)-activated and voltage-sensitive K(+) channel inhibitor tetraethylammonium (1 mM), adenosine triphosphate-sensitive K(+) channel inhibitor glibenclamide (10 microM), voltage-dependent inward rectifier K(+) channel inhibitor barium chloride (30 microM) and voltage-sensitive K(+) channel inhibitor 4-aminopyridine (1 mM).


RESULTS
Testosterone (0.1-300 microM) produced relaxation in human corpus cavernosum (maximum relaxation 65.4% +/- 3.3% of KCl-induced contraction) that reached a maximum at a concentration of 300 microM. Testosterone-induced relaxation was significantly attenuated by glibenclamide, but it was not affected by the other K(+) channel inhibitors (tetraethylammonium, barium chloride, or 4-aminopyridine).


CONCLUSIONS
Testosterone might induce relaxation in human isolated corpora cavernosa strips by activation of smooth muscle adenosine triphosphate-sensitive K(+) channels. This finding suggests that testosterone, in addition to its known endothelial action, might regulate erectile function locally by its action on the smooth muscle of the human corpus cavernosum.
Yildiz
Oguzhan
O
Department of Pharmacology, Gulhane School of Medicine, Ankara, Turkey. oyildiz@gata.edu.tr
Seyrek
Melik
M
Irkilata
Hasan Cem
HC
Yildirim
Ibrahim
I
Tahmaz
Lutfu
L
Dayanc
Murat
M
D006801:Humans; D066298:In Vitro Techniques; D008297:Male; D009126:Muscle Relaxation; D009130:Muscle, Smooth / Q000502:physiology; D010413:Penis / Q000502:physiology*; D015221:Potassium Channels / Q000502:physiology*; D013739:Testosterone / Q000502:physiology*
D015221:Potassium Channels; D013739:Testosterone