Epitope mapping of antibody-antigen complexes by nuclear magnetic resonance spectroscopy.
Nuclear magnetic resonance (NMR) is a very powerful tool for determining the boundaries of peptide epitopes recognized by antibodies. NMR can be used to study antibodies in complexes that exhibit a wide range of binding affinities from very weak and transient to very tight. Choice of the specific method depends upon the dissociation constant, especially the ligand off-rate.Epitope mapping by NMR is based on the difference in mobility between the amino acid residues of a peptide antigen that interact tightly with the antibody and residues outside the epitope that do not interact with the antibody. The interacting peptide residues become considerably immobilized upon binding. Their mobility will resemble that of the antibody's residues. Several NMR methods were developed based on these characteristics. In this chapter we discuss some of these methods, including dynamic filtering, comparison of (1)H-(15)N HSQC peaks' intensities, transverse relaxation time, measurements of (1)H-(15)N nuclear Overhauser effect (NOE) values, and measurements of T (1rho) relaxation time.
Department of Structural Biology, Weizmann Institute of Science, Rehovot 76100, Israel.
Osnat
O
Rosen
Jacob
J
Anglister
D000818:Animals; D000906:Antibodies / Q000032:analysis / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification; D000936:Antigen-Antibody Complex / Q000032:analysis* / Q000276:immunology*; D001666:Binding Sites, Antibody; D018604:Epitope Mapping / Q000379:methods*; D004926:Escherichia coli / Q000235:genetics; D006801:Humans; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods*; D010455:Peptides / Q000032:analysis / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification
D000906:Antibodies; D000936:Antigen-Antibody Complex; D010455:Peptides