Decreased somatostatin is related to the hypersensitivity of intestinal epithelia to LPS via upregulated TLR4-TBK1 pathway in rats chronically exposed to ethanol.
Chronic alcoholics are predisposed to the development of a systemic inflammatory response syndrome, which is usually triggered in the gut. This study aimed to investigate in rats the role of intestinal epithelial inflammatory responsiveness in the susceptibility of alcoholics to excessive inflammation. Thirty Wistar rats were randomly divided into three groups: 10 rats killed immediately after acclimation (baseline control), 10 rats treated with 25% (vol/vol) ethanol for 6 months (ethanol group), and 10 rats given double-distilled water until killed simultaneously with the ethanol group (9-month control). The intestinal microflora, the epithelial histology and ultrastructure, the level of Toll-like receptor 4 (TLR4), TANK-binding kinase-1 (TBK1), and activated nuclear factor-kappaB (NF-kappaB) in the intestinal mucosa, and somatostatin (SST) levels in plasma and small intestine were evaluated in each group. Isolated intestinal epithelia from each rat were used to examine lipopolysaccharide (LPS) responsiveness with or without SST pretreatment by quantification of TLR4, TBK1, activated NF-kappaB, interferon-gamma (IFN-gamma), and tumor necrosis factor-alpha (TNF-alpha). Compared with both the control groups, the amount of mucosal Escherichia coli in the ethanol group was not changed, whereas the number of intestinal lactobacilli in the ethanol group was significantly reduced (P<.05). Mild inflammatory injury and upregulation of TLR4 and TBK1 were observed in the intestinal mucosa of the ethanol group. The LPS-enhanced in vitro expression of TLR4, TBK1, and production of IFN-gamma and TNF-alpha in isolated intestinal epithelia of the ethanol group were significantly higher than those in either control group (P<.05) and were dramatically inhibited by SST (P<.05), whereas NF-kappaB was activated by LPS only in the control groups. The plasma and intestinal levels of SST in the ethanol group were significantly lower than those in either control group (P<.05). These findings suggest that impairment of intestinal SST production by chronic ethanol administration leading to upregulation of the TLR4-TBK1 pathway may be one of the mechanisms underlying the LPS hypersensitivity of intestinal epithelia.
Zhou
Chao
C
Department of Gastroenterology, West China Hospital, Sichuan University, Chengdu, PR China.
Li
Jing
J
Wang
Haiying
H
Tang
Chengwei
C
D000818:Animals; D000431:Ethanol / Q000008:administration & dosage*; D007413:Intestinal Mucosa / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D008070:Lipopolysaccharides / Q000633:toxicity*; D008297:Male; D017346:Protein Serine-Threonine Kinases / Q000096:biosynthesis*; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D015398:Signal Transduction / Q000187:drug effects / Q000502:physiology*; D013004:Somatostatin / Q000037:antagonists & inhibitors* / Q000378:metabolism; D051197:Toll-Like Receptor 4 / Q000096:biosynthesis* / Q000502:physiology; D015854:Up-Regulation / Q000187:drug effects / Q000502:physiology*
D008070:Lipopolysaccharides; C495347:Tlr4 protein, rat; D051197:Toll-Like Receptor 4; D000431:Ethanol; D013004:Somatostatin; D017346:Protein Serine-Threonine Kinases; C403191:TBK1 protein, human