Thermoresponsive poly(N-isopropylacrylamide) gel for immobilization of laccase on indium tin oxide electrodes.
We report on the properties of hydrogel matrix for the immobilization of laccase on conductive supports. The poly(N-isopropylacrylamide) gel is attached firmly to the indium-tin oxide (ITO) electrode, following its silanization with dimethylethoxyvinylsilane. The enzyme entrapped in the gel structure remained active longer than in the solution, and its redox and catalytic properties could be investigated by voltammetric methods. The reduction signals of the active sites, T1 and T2, of the Cerrena unicolor laccase were determined to be 0.79 and 0.38 V, respectively. The laccase catalytic activity toward oxygen in poly(N-isopropylacrylamide) was found to depend strongly on temperature. Reversible swelling/shrinking of the matrix was studied at 30 and 35 degrees C. Shrinking of the gel at higher temperature considerably decreased the efficiency of the catalytic reaction, however, interestingly, did not lead to irreversible changes in the enzyme structure. At temperatures below that corresponding to volume phase transition, the catalytic properties of the film were fully restored. High catalytic efficiency of the gel immobilized enzyme made it possible to employ the gel covered electrode for monitoring oxygen in solutions.
Faculty of Chemistry, University of Warsaw, Pasteura 1, 02-093 Warsaw, Poland.
Maciej
M
Klis
Marcin
M
Karbarz
Zbigniew
Z
Stojek
Jerzy
J
Rogalski
Renata
R
Bilewicz
D000178:Acrylamides / Q000737:chemistry*; D000180:Acrylic Resins; D002384:Catalysis; D020134:Catalytic Domain; D004563:Electrochemistry; D004566:Electrodes; D004800:Enzymes, Immobilized / Q000737:chemistry*; D005782:Gels / Q000737:chemistry; D042845:Laccase / Q000737:chemistry*; D010084:Oxidation-Reduction; D011108:Polymers / Q000737:chemistry*; D013499:Surface Properties; D013696:Temperature; D017971:Tin Compounds / Q000737:chemistry*
D000178:Acrylamides; D000180:Acrylic Resins; D004800:Enzymes, Immobilized; D005782:Gels; D011108:Polymers; D017971:Tin Compounds; C052970:poly-N-isopropylacrylamide; C109984:indium tin oxide; D042845:Laccase