Antioxidant potential of the root of Vetiveria zizanioides (L.) Nash.
Vetiveria zizanioides, an aromatic plant commonly known as vetiver has been used for various ailments. The essential oil of vetiver root has been shown to possess antioxidant activity. However, antioxidant potential of spent root extract has not been reported. Hence, in the present study, ferric reducing, free radical scavenging and antioxidant activity of two genotypes namely KS1 and gulabi of V. zizanioides L. Nash root were investigated using in vitro assays - the ferric reducing antioxidant power (FRAP), 1,1-diphenyl-2-picrylhydrazyl (DPPH), total phenolic content (TPC), total antioxidant capacity (TAC) and reducing power (RP). KS1 genotype showed higher FRAP values, DPPH inhibition, TPC and RP potential compared to gulabi and the antioxidant activity increased with the concentration of the extract (10-1000 microg/mL). A significant protective effect of cv KS1 (100 microg/mL) extract was also observed in reduced glutathione (GSH) and malondialdehyde (MDA) concentrations of erythrocytes subjected to oxidative stress by tert-butyl hydroperoxide (t-BHP) and hydrogen peroxide (H202). The cv KS1 showed better antioxidant activity, compared to cv gulabi indicating the possibility of exploring the presence of different phytoconstituents in the two varieties.
Luqman
Suaib
S
Genetic Resources and Biotechnology Division, Central Institute of Medicinal and Aromatic Plants (Council of Scientific and Industrial Research), P.O. CIMAP, Lucknow 226 015, India s.luqman@cimap.res.in
Kumar
Ritesh
R
Kaushik
Shubhangi
S
Srivastava
Suchita
S
Darokar
Mahendra P
MP
Khanuja
Suman P S
SP
D000975:Antioxidants / Q000494:pharmacology*; D001713:Biphenyl Compounds; D049930:Chrysopogon / Q000737:chemistry; D004912:Erythrocytes / Q000187:drug effects / Q000502:physiology; D005838:Genotype; D005978:Glutathione / Q000378:metabolism; D006861:Hydrogen Peroxide; D062385:Hydroxybenzoates / Q000032:analysis; D008315:Malondialdehyde / Q000378:metabolism; D010084:Oxidation-Reduction; D018384:Oxidative Stress / Q000187:drug effects / Q000502:physiology; D010851:Picrates; D010936:Plant Extracts / Q000494:pharmacology*; D020122:tert-Butylhydroperoxide
D000975:Antioxidants; D001713:Biphenyl Compounds; D062385:Hydroxybenzoates; D010851:Picrates; D010936:Plant Extracts; D008315:Malondialdehyde; D020122:tert-Butylhydroperoxide; D006861:Hydrogen Peroxide; C004931:1,1-diphenyl-2-picrylhydrazyl; D005978:Glutathione; C017616:phenolic acid