Anti-inflammatory effect of peroxisome proliferator activated receptor gamma on human dental pulp cells.
Peroxisome proliferator activated receptor gamma (PPARgamma) plays a critical role in controlling immune and inflammatory responses. However, its effect on pulpal inflammation has not been clarified. The purpose of this study was to determine the anti-inflammatory effect of PPARgamma on pulpal inflammation. Human dental pulp cells treated with lipopolysaccharide exhibited elevated levels of matrix metalloproteinase-2 (MMP-2), MMP-9, intracellular adhesion molecule-1 (ICAM-1), and vascular cell adhesion molecule-1 (VCAM-1). However, when treated with rosiglitazone (PPARgamma agonist) or adenoviral PPARgamma in same culture system, the expression of ICAM-1 and VCAM-1 was markedly inhibited along with decreased secretion of MMPs. In addition, the coadministration of GW9662 (PPARgamma antagonist) and rosiglitazone blocked the inhibition of MMP-2, MMP-9, ICAM-1, and VCAM-1. These results suggest that PPARgamma decreased the production of MMPs, ICAM-1, and VCAM-1 and might offer a possible attempt of using it as one of anti-inflammatory modulators in a pulpal inflammation.
Department of Conservative Dentistry, School of Dentistry and Institute of Oral-Bio Science, Chonbuk National University, Jeonju, Jeonbuk, Korea.
Mi-Kyung
MK
Yu
Jung-Chang
JC
Lee
Jeong-Hee
JH
Kim
Young-Hee
YH
Lee
Jae-Gyu
JG
Jeon
Eun-Chung
EC
Jhee
Ho-Keun
HK
Yi
D000813:Anilides / Q000494:pharmacology; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D015153:Blotting, Western; D002478:Cells, Cultured; D003782:Dental Pulp / Q000166:cytology / Q000378:metabolism*; D004926:Escherichia coli / Q000737:chemistry; D005347:Fibroblasts / Q000378:metabolism; D006801:Humans; D018836:Inflammation Mediators / Q000378:metabolism*; D018799:Intercellular Adhesion Molecule-1 / Q000096:biosynthesis; D008070:Lipopolysaccharides; D020778:Matrix Metalloproteinase 2 / Q000096:biosynthesis; D020780:Matrix Metalloproteinase 9 / Q000096:biosynthesis; D061965:Matrix Metalloproteinase Inhibitors; D047495:PPAR gamma / Q000819:agonists / Q000037:antagonists & inhibitors / Q000494:pharmacology / Q000502:physiology*; D011671:Pulpitis / Q000378:metabolism*; D011994:Recombinant Proteins / Q000494:pharmacology; D000077154:Rosiglitazone; D045162:Thiazolidinediones / Q000494:pharmacology; D019010:Vascular Cell Adhesion Molecule-1 / Q000096:biosynthesis
C457499:2-chloro-5-nitrobenzanilide; D000813:Anilides; D000893:Anti-Inflammatory Agents; D018836:Inflammation Mediators; D008070:Lipopolysaccharides; D061965:Matrix Metalloproteinase Inhibitors; D047495:PPAR gamma; D011994:Recombinant Proteins; D045162:Thiazolidinediones; D019010:Vascular Cell Adhesion Molecule-1; D000077154:Rosiglitazone; D018799:Intercellular Adhesion Molecule-1; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9