Arabidopsis chloroplastic glutathione peroxidases play a role in cross talk between photooxidative stress and immune responses.
Glutathione peroxidases (GPXs; EC 1.11.1.9) are key enzymes of the antioxidant network in plants and animals. In order to investigate the role of antioxidant systems in plant chloroplasts, we generated Arabidopsis (Arabidopsis thaliana) transgenic lines that are depleted specifically in chloroplastic (cp) forms of GPX1 and GPX7. We show that reduced cpGPX expression, either in transgenic lines with lower total cpGPX expression (GPX1 and GPX7) or in a gpx7 insertion mutant, leads to compromised photooxidative stress tolerance but increased basal resistance to virulent bacteria. Depletion of both GPX1 and GPX7 expression also caused alterations in leaf cell and chloroplast morphology. Leaf tissues were characterized by shorter and more rounded palisade cells, irregular spongy mesophyll cells, and larger intercellular air spaces compared with the wild type. Chloroplasts had larger and more abundant starch grains than in wild-type and gpx7 mutant plants. Constitutively reduced cpGPX expression also led to higher foliar ascorbic acid, glutathione, and salicylic acid levels in plants exposed to higher light intensities. Our results suggest partially overlapping functions of GPX1 and GPX7. The data further point to specific changes in the chloroplast ascorbate-glutathione cycle due to reduced cpGPX expression, initiating reactive oxygen species and salicylic acid pathways that affect leaf development, light acclimation, basal defense, and cell death programs. Thus, cpGPXs regulate cellular photooxidative tolerance and immune responses.
Department of Botany, Stockholm University, Frescati 10691 Stockholm, Sweden.
Christine C C
CC
Chang
Ireneusz
I
Slesak
Lucía
L
Jordá
Alexey
A
Sotnikov
Michael
M
Melzer
Zbigniew
Z
Miszalski
Philip M
PM
Mullineaux
Jane E
JE
Parker
Barbara
B
Karpinska
Stanislaw
S
Karpinski
D000222:Adaptation, Physiological / Q000528:radiation effects; D000975:Antioxidants / Q000378:metabolism; D017360:Arabidopsis / Q000166:cytology / Q000201:enzymology* / Q000235:genetics / Q000276:immunology*; D016923:Cell Death / Q000528:radiation effects; D002736:Chloroplasts / Q000201:enzymology* / Q000528:radiation effects / Q000648:ultrastructure; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D005979:Glutathione Peroxidase / Q000235:genetics / Q000378:metabolism*; D006861:Hydrogen Peroxide / Q000378:metabolism; D008027:Light; D009154:Mutation / Q000235:genetics; D018384:Oxidative Stress / Q000528:radiation effects*; D018515:Plant Leaves / Q000166:cytology / Q000201:enzymology / Q000528:radiation effects / Q000648:ultrastructure; D030821:Plants, Genetically Modified; D020156:Salicylic Acid / Q000378:metabolism; D013312:Stress, Physiological / Q000528:radiation effects
D000975:Antioxidants; D006861:Hydrogen Peroxide; D005979:Glutathione Peroxidase; D020156:Salicylic Acid