A unique group I intron in Coxiella burnetii is a natural splice mutant.
Cbu.L1917, a group I intron present in the 23S rRNA gene of Coxiella burnetii, possesses a unique 3'-terminal adenine in place of a conserved guanine. Here, we show that, unlike all other group I introns, Cbu.L1917 utilizes a different cofactor for each splicing step and has a decreased self-splicing rate in vitro.
Division of Biological Sciences, The University of Montana, Missoula, MT 59812, USA.
Rahul
R
Raghavan
Linda D
LD
Hicks
Michael F
MF
Minnick
D016997:Coxiella burnetii / Q000235:genetics*; D007438:Introns; D009154:Mutation; D012326:RNA Splicing; D012329:RNA, Bacterial / Q000235:genetics; D012338:RNA, Ribosomal, 23S / Q000235:genetics
D012329:RNA, Bacterial; D012338:RNA, Ribosomal, 23S