Effects of a nutrient mixture on infectious properties of the highly pathogenic strain of avian influenza virus A/H5N1.
Numerous outbreaks of avian influenza virus infection (A/H5N1) have occurred recently, infecting domestic birds, chicken and ducks. The possibility of the emergence of a new strain of influenza virus capable of causing a pandemic in humans is high and no vaccine effective against such a strain currently exists. A unique nutrient mixture (NM), containing lysine, proline, ascorbic acid, green tea extract, N-acetyl cysteine, selenium among other micro nutrients, has been shown to exert a wide range of biochemical and pharmacological effects, including an inhibitory effect on replication of influenza virus and HIV. This prompted us to investigate the potential anti-viral activity of a nutrient mixture (NM) and its components on avian influenza virus A/H5N1at viral dosages of 1.0, 0.1 and 0.01 TCID(50). Antiviral activity was studied in cultured cell lines PK, BHK-21, and Vero-E6. Virus lysing activity was determined by co-incubation of virus A/H5N1 with NM for 0-60 min, followed residual virulence titration in cultured SPEV or BHK-21 cells. NM demonstrated high antiviral activity evident even at prolonged periods after infection. NM antiviral properties were comparable to those of conventional drugs (amantadine and oseltamivir); however, NM had the advantage of affecting viral replication at the late stages of the infection process.
Russian Academy of Medical Sciences, D.I. Ivanovsky Research Institute on Virology, USA.
Petr G
PG
Deryabin
Dmitry K
DK
Lvov
Andrey G
AG
Botikov
Vadim
V
Ivanov
Tatiana
T
Kalinovsky
Aleksandra
A
Niedzwiecki
Matthias
M
Rath
D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology*; D001205:Ascorbic Acid / Q000494:pharmacology; D002478:Cells, Cultured; D002522:Chlorocebus aethiops; D006801:Humans; D053124:Influenza A Virus, H5N1 Subtype / Q000187:drug effects* / Q000472:pathogenicity*; D008239:Lysine / Q000494:pharmacology; D018977:Micronutrients / Q000494:pharmacology*; D010936:Plant Extracts / Q000494:pharmacology; D011392:Proline / Q000494:pharmacology; D012643:Selenium / Q000494:pharmacology; D013662:Tea; D014709:Vero Cells
D000998:Antiviral Agents; D018977:Micronutrients; D010936:Plant Extracts; D013662:Tea; D011392:Proline; D012643:Selenium; D008239:Lysine; D001205:Ascorbic Acid