The R2 mobile element of Rhynchosciara americana: molecular, cytological and dynamic aspects.
Ribosomal RNA genes are encoded by large units clustered (18S, 5S, and 28S) in the nucleolar organizer region in several organisms. Sometimes additional insertions are present in the coding region for the 28S rDNA. These insertions are specific non-long terminal repeat retrotransposons that have very restricted integration targets within the genome. The retrotransposon present in the genome of Rhynchosciara americana, RaR2, was isolated by the screening of a genomic library. Sequence analysis showed the presence of conserved regions, such as a reverse transcriptase domain and a zinc finger motif in the amino terminal region. The insertion site was highly conserved in R. americana and a phylogenetic analysis showed that this element belongs to the R2 clade. The chromosomal localization confirmed that the RaR2 mobile element was inserted into a specific site in the rDNA gene. The expression level of RaR2 in salivary glands during larval development was determined by quantitative RT-PCR, and the increase of relative expression in the 3P of the fourth instar larval could be related to intense gene activity characteristic of this stage. 5'-Truncated elements were identified in different DNA samples. Additionally, in three other Rhynchosciara species, the R2 element was present as a full-length element.
Departamento de Biologia Celular e Desenvolvimento, Instituto de Ciências Biomédicas, Universidade de São Paulo, São Paulo, SP, Brazil. paularez@usp.br
Paula
P
Rezende-Teixeira
Fábio
F
Siviero
Marina
M
da Costa Rosa
Glaucia Maria
GM
Machado-Santelli
D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002875:Chromosomes; D017124:Conserved Sequence; D004175:Diptera / Q000235:genetics*; D005260:Female; D020459:Genes, rRNA; D016678:Genome; D015698:Genomic Library; D017403:In Situ Hybridization; D008297:Male; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D016366:Open Reading Frames; D010053:Ovary / Q000166:cytology; D010802:Phylogeny; D017434:Protein Structure, Tertiary; D012335:RNA, Ribosomal / Q000235:genetics*; D018626:Retroelements / Q000235:genetics*; D012469:Salivary Glands / Q000166:cytology; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D013737:Testis / Q000166:cytology
D012335:RNA, Ribosomal; D018626:Retroelements