Metabolite analysis in Curcuma domestica using various GC-MS and LC-MS separation and detection techniques.
The metabolic profile of polar (methanol) and non-polar (hexane) extracts of Curcuma domestica, a widely used medicinal plant, was established using various different analytical techniques, including GC-FID, GC-MS, HR-GC-MS and analytical HPLC-ESI-MS/MS by means of LTQ-Orbitrap technology. The major non-volatile curcuminoids curcumin, demethoxycurcumin and bisdemethoxycurcumin were identified when their chromatographic and precursor ion masses were compared with those of authentic standard compounds. In this paper we describe for the first time a GC/MS-based method for metabolic profiling of the hydrophilic extract. We also identified 61 polar metabolites as TMS derivatives.
Institute of Environmental Research, University of Dortmund, Dortmund, Germany.
Diran
D
Herebian
Jeong-Heui
JH
Choi
A M
AM
Abd El-Aty
Jae-Han
JH
Shim
Michael
M
Spiteller
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D030024:Curcuma / Q000737:chemistry* / Q000378:metabolism*; D003474:Curcumin / Q000031:analogs & derivatives / Q000032:analysis / Q000302:isolation & purification; D036381:Diarylheptanoids; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006586:Hexanes / Q000737:chemistry; D000432:Methanol / Q000737:chemistry; D010936:Plant Extracts / Q000032:analysis* / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
D036381:Diarylheptanoids; D006586:Hexanes; D010936:Plant Extracts; C034786:bisdemethoxycurcumin; D003474:Curcumin; C050229:demethoxycurcumin; D000432:Methanol