Evaluation of unexpected positive results from a commercial ELISA for antibodies to PRRSV.
Unexpected positive results from the widely used IDEXX ELISA for the detection of antibodies to porcine reproductive and respiratory syndrome virus (PRRSV) may confound investigations of the disease. Supplementing the ELISA with blocking agents and the use of IgG purified from serum samples had no effect on the unexpected positive results, suggesting that they were due to an antibody-antigen reaction. Simple competitive and blocking ELISAs were developed by modifying the IDEXX ELISA, and they and an indirect fluorescent antibody test (IFAT) were used to examine PRRSV antibodies in 33 antibody-negative, 88 antibody-positive and 73 unexpectedly positive sera. All the unexpectedly positive sera were negative by IFAT, and 89.0 per cent were negative by both the competitive and blocking ELISAs. The competitive ELISA (97.7 per cent) and the blocking ELISA (96.5 per cent) detected more positive sera than the IFAT (90.9 per cent). These results show that both ELISAs are capable of distinguishing positive and unexpectedly positive sera, and suggest that most of the unexpected positive signals are false-positives.
Okinaga
T
T
Viral Diseases Research Team, National Institute of Animal Health, Kannondai, Tsukuba, Ibaraki 305-0856, Japan.
Yamagishi
T
T
Yoshii
M
M
Suzuki
T
T
Miyazaki
A
A
Takagi
M
M
Tsunemitsu
H
H
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000592:standards / Q000662:veterinary*; D005189:False Positive Reactions; D019084:Fluorescent Antibody Technique, Indirect / Q000662:veterinary; D019318:Porcine Reproductive and Respiratory Syndrome / Q000175:diagnosis*; D019316:Porcine respiratory and reproductive syndrome virus / Q000276:immunology*; D012680:Sensitivity and Specificity; D013552:Swine
D000914:Antibodies, Viral