Synaptotagmin I stabilizes synaptic vesicles via its C(2)A polylysine motif.
The synaptic vesicle protein, synaptotagmin I, is a multifunctional protein required for several steps in the synaptic vesicle cycle. It is primarily composed of two calcium-binding domains, C(2)A and C(2)B. Within each of these domains, a polylysine motif has been identified that is proposed to mediate specific functions within the synaptic vesicle cycle. While the C(2)B polylysine motif plays an important role in synaptic transmission in vivo, the C(2)A polylysine motif has not previously been analyzed at an intact synapse. Here, we show that mutation of the C(2)A polylysine motif increases the frequency of spontaneous transmitter release in vivo. The increased frequency is not a developmental consequence of disrupted synaptic transmission, as evoked transmitter release is unimpaired in the mutants. Our results demonstrate that synaptotagmin I plays a direct role in regulating spontaneous transmitter release, indicative of an active role in synaptic vesicle stabilization mediated by the C(2)A polylysine motif.
Department of Biomedical Sciences, Colorado State University, Fort Collins, 80523-1617, USA.
Kimberly E
KE
Mace
Laurie M
LM
Biela
Anastasia G
AG
Sares
Noreen E
NE
Reist
D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites / Q000235:genetics; D002118:Calcium / Q000378:metabolism*; D004331:Drosophila melanogaster / Q000235:genetics / Q000378:metabolism / Q000648:ultrastructure; D004594:Electrophysiology; D019706:Excitatory Postsynaptic Potentials; D015151:Immunoblotting; D008854:Microscopy, Electron; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009154:Mutation; D011107:Polylysine / Q000235:genetics*; D017434:Protein Structure, Tertiary; D017386:Sequence Homology, Amino Acid; D009435:Synaptic Transmission; D013572:Synaptic Vesicles / Q000378:metabolism* / Q000502:physiology; D050863:Synaptotagmin I / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*
D050863:Synaptotagmin I; D011107:Polylysine; D002118:Calcium