First gene cassettes of integrons as targets in finding adaptive genes in metagenomes.
The first gene cassettes of integrons are involved in the last adaptation response to changing conditions and are also the most expressed. We propose a rapid method for the selection of clones carrying an integron first gene cassette that is useful for finding adaptive genes in environmental metagenomic libraries.
Université de Pau et des Pays de l'Adour, IPREM UMR CNRS, IBEAS, BP, Pau, France.
Lionel
L
Huang
Christine
C
Cagnon
Pierre
P
Caumette
Robert
R
Duran
D000220:Adaptation, Biological; D001419:Bacteria / Q000235:genetics*; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D032023:Integrons; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA
D004269:DNA, Bacterial