[Acute cardiac injury activates interleukin-1 beta signaling in the spinal cord].
OBJECTIVE
To investigate the effect of acute cardiac injury on the activation of interleukin-1 beta (IL-1 beta) signaling in the spinal cord.


METHODS
Acute cardiac injury rat model was established by intra-myocardial injection of formalin through diaphragm. IL-1 beta expression was determined by Western blot, immunohistochemistry and in situ hybridization. The DNA binding activities of 2 IL-1 beta transcription factors, activator protein (AP)-1 and nuclear factor kB (NF-kappaB) were measured by electrophoretic mobility shift assay (EMSA).


RESULTS
After cardiac injury, the IL-1 beta protein level was dramatically upregulated in the spinal cord. The upregulated IL-1 beta was mainly expressed in the neurons in the lamina II approximately IV of the spinal cord. In response to cardiac injury, the DNA binding activities of NF-kappaB and AP-1 were greatly activated.


CONCLUSIONS
Acute cardiac injury could activate the spinal IL-1 beta signaling, which, in turn, may be involved in the progression of heart failure after injury.
Department of Anesthesia, Second Xiangya Hospital, Central South University, Changsha 410011, China.
Jianhua
J
Liu
Di
D
Fu
Junmei
J
Xu
Xianghang
X
Lu
Ruping
R
Dai
D000818:Animals; D053583:Interleukin-1beta / Q000235:genetics / Q000378:metabolism*; D008297:Male; D015428:Myocardial Reperfusion Injury / Q000139:chemically induced / Q000378:metabolism* / Q000473:pathology; D009206:Myocardium / Q000378:metabolism / Q000473:pathology*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D015398:Signal Transduction; D013116:Spinal Cord / Q000378:metabolism*
D053583:Interleukin-1beta; D012333:RNA, Messenger