Performance characteristics of a monoclonal antibody cocktail-based ELISA for detection of allergen-specific IgE in dogs and comparison with a high affinity IgE receptor-based ELISA.
The purpose of this study was to define the operational and performance characteristics of a commercially available monoclonal antibody based (mac) ELISA for detection of allergen-specific IgE in dogs. The average intra-assay variance over 1 year was 9.7% (range 2.5-62.7%), while the interassay variance averaged 10.8% (range 8.1-13.8%). The average positive control responses observed for grass, weed, tree and mite allergens during each month remained relatively constant; the average monthly variance was 11.6% (range 8.3-19.2%) for grass pollens, 13.3% (range 9.1-20.4%) for weed pollens, 13.3% (range 9.8-18.2%) for tree pollens and 13.6% (range 8.9-18.7%) for mite allergens. The interlaboratory concordance of results for the macELISA was approximately 91%. The interlaboratory concordance of results comparing the macELISA and a high affinity IgE receptor-based ELISA was approximately 92%. The results demonstrate that the macELISA is reproducible and the results are comparable to the high affinity IgE receptor based ELISA within and between laboratories.
Lee
Kenneth W
KW
Greer Laboratories, Inc., Lenoir, North Carolina 28645, USA. klee@greerlabs.com
Blankenship
Karen D
KD
McCurry
Zachary M
ZM
Esch
Robert E
RE
DeBoer
Douglas J
DJ
Marsella
Rosanna
R
D000485:Allergens / Q000276:immunology*; D000818:Animals; D000911:Antibodies, Monoclonal; D002138:Calibration; D019411:Clinical Laboratory Techniques / Q000592:standards / Q000662:veterinary; D003876:Dermatitis, Atopic / Q000175:diagnosis / Q000276:immunology / Q000662:veterinary*; D004283:Dog Diseases / Q000175:diagnosis* / Q000276:immunology; D004285:Dogs; D004797:Enzyme-Linked Immunosorbent Assay / Q000592:standards / Q000662:veterinary*; D007073:Immunoglobulin E / Q000097:blood / Q000276:immunology*; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D000485:Allergens; D000911:Antibodies, Monoclonal; D007073:Immunoglobulin E