[Association of the integrin gene polymorphisms with ischemic stroke and plasma lipid levels].
OBJECTIVE
To study the association of integrin alpha-2 (ITGA2) gene C807T, integrin beta-3 (ITGB3) gene T176C polymorphisms with ischemic stroke and the effect of the polymorphisms on plasma lipid and lipoprotein levels.


METHODS
Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and DNA sequencing were used to detect the integrin genotypes in 265 patients with ischemic stroke and 280 healthy controls. The plasma lipid and lipoprotein levels were measured by routine method.


RESULTS
Plasma total cholesterol (TC), triacylglycerol (TG) and low density lipoprotein-cholesterol (LDL-C) in the patients with ischemic stroke were significantly higher than those in the controls (P< 0.05). The distributions of the ITGB3 gene T176C polymorphism were not different between the ischemic stroke group and control group, but the ITGA2 gene C807T polymorphism was significantly different. The relative risk suffering from ischemic stroke of the T allele carrier was 1.455 times as that of the C allele carrier (OR=1.455, 95%CI: 1.134-1.866). The level of plasma lipid in the T allele carriers was significantly higher than that in the C allele carriers (P< 0.05).


CONCLUSIONS
The ITGA2 gene C807T polymorphism was associated with ischemic stroke, the 807 T allele may be a genetic risk factor for ischemic stroke. The ITGA2 gene C807T polymorphism may affect ischemic stroke through plasma lipid and lipoprotein levels.
The Affiliated Hospital of Youjiang Medical College for Nationalities, Baise, Guangxi, People's Republic of China. weiyesheng@yahoo.com.cn
Ye-sheng
YS
Wei
Yan
Y
Lan
Yun-guang
YG
Liu
Lan-qing
LQ
Meng
Qun-qing
QQ
Xu
Hai-yuan
HY
Xie
D002545:Brain Ischemia / Q000097:blood / Q000235:genetics / Q000378:metabolism*; D008078:Cholesterol, LDL / Q000235:genetics / Q000378:metabolism; D005260:Female; D020022:Genetic Predisposition to Disease; D006801:Humans; D039421:Integrin alpha2 / Q000235:genetics / Q000378:metabolism*; D039661:Integrin beta3 / Q000235:genetics / Q000378:metabolism*; D050356:Lipid Metabolism / Q000235:genetics; D008055:Lipids / Q000097:blood*; D008297:Male; D008875:Middle Aged; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide
D008078:Cholesterol, LDL; C061248:ITGB3 protein, human; D039421:Integrin alpha2; D039661:Integrin beta3; D008055:Lipids