The immunoglobulin G subclass distribution of anti-GBM autoantibodies against rHalpha3(IV)NC1 is associated with disease severity.
The current study aimed to investigate the association between antiglomerular basement membrane (GBM) immunoglobulin (Ig) G subclass distribution and the severity of anti-GBM nephritis in human beings. Natural anti-GBM IgG affinity purified from five healthy donors was defined as group A. A total of 73 Chinese patients with anti-GBM disease were divided into three groups (groups B, C, and D) according to their serum creatinine (Scr) on diagnosis. Anti-GBM IgG subclasses were detected by enzyme-linked immunosorbent assay using recombinant human noncollagenous domain 1 of type IV collagen of the alpha3 chain [rHalpha3(IV)NC1]. Anti-GBM IgG1 could not be detected in group A (0%), appeared in group B (7.7%), was significantly higher in group C (69%), and was the highest in group D (93.5%). The frequency of IgG3 in groups C and D was significantly higher than that in groups A and B. In group A, anti-GBM IgG subclass distribution was restricted to IgG4 and IgG2; in group B, IgG2 and IgG4 remained the major subclasses with detectable IgG1; in group C, IgG1 became predominant and IgG3 was detectable; in group D, four IgG subclasses could be detected. In conclusion, anti-GBM autoantibody IgG subclass distribution was associated with disease severity. IgG1 and IgG3 may play a major role in the initiation and progression of the disease.
Renal Division, Department of Medicine, Peking University First Hospital, Institute of Nephrology, Peking University, Key Laboratory of Renal Disease, Ministry of Health of China, Beijing 100034, People's Republic of China.
Juan
J
Zhao
Yu
Y
Yan
Zhao
Z
Cui
Rui
R
Yang
Ming-Hui
MH
Zhao
D000328:Adult; D000368:Aged; D000818:Animals; D019867:Anti-Glomerular Basement Membrane Disease / Q000276:immunology* / Q000503:physiopathology; D001323:Autoantibodies / Q000097:blood / Q000276:immunology*; D001324:Autoantigens / Q000276:immunology*; D002417:Cattle; D002460:Cell Line; D024141:Collagen Type IV / Q000276:immunology*; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood*; D008297:Male; D008875:Middle Aged; D011994:Recombinant Proteins / Q000276:immunology; D055815:Young Adult
D001323:Autoantibodies; D001324:Autoantigens; D024141:Collagen Type IV; D007074:Immunoglobulin G; D011994:Recombinant Proteins; C051951:antiglomerular basement membrane antibody; C057112:type IV collagen alpha3 chain