Evaluation of biodegradation of nonylphenol ethoxylate and lignin by combining toxicity assessment and chemical characterization.
The aerobic biodegradation of commercial nonylphenol ethoxylate (NPE) mixture and alkali lignin was studied using the OECD headspace test accompanied by the simultaneous measurement of ecotoxicity directly from the biodegradation liquors and by the follow-up of the chemical composition of the studied chemicals. NPE degradation was dependent on the inoculum source: approximately 40% of NPE was mineralized into CO(2) during the 4-week experiment when inoculum from Helsinki City wastewater treatment plant (WWTP) was used, and only 12% was mineralized when inoculum from Jyväskylä City WWTP was used. Chemical analyses revealed a shift in the ethoxylate chain length from longer to shorter soon after the beginning of the NPE biodegradation tests. At the same time also toxicity (reverse electron transport assay, RET) and estrogenic activity (human estrogen receptor yeast) measured directly from the biodegradation liquors decreased. In case of alkali lignin, approximately 11% was mineralized in the test and chemical analysis showed in maximum a 30% decrease in lignin concentration. Toxicity of lignin biodegradation liquors started to decrease in the beginning of the test, but became more toxic towards the end of the test again. Especially RET assay proved to be sensitive enough for measuring toxicity changes directly from biodegradation liquors, although a concentrating treatment of the liquors is recommended for a more detailed characterization and identification of toxic metabolites.
Pessala
P
P
Finnish Environment Institute, PO Box 140, FI-00251 Helsinki, Finland.
Keränen
J
J
Schultz
E
E
Nakari
T
T
Karhu
M
M
Ahkola
H
H
Knuutinen
J
J
Herve
S
S
Paasivirta
J
J
Ahtiainen
J
J
D001673:Biodegradation, Environmental; D001681:Biological Assay; D054750:Ecotoxicology; D004579:Electron Transport; D004967:Estrogens / Q000378:metabolism; D005026:Ethylene Glycols / Q000737:chemistry / Q000378:metabolism* / Q000633:toxicity; D008031:Lignin / Q000737:chemistry / Q000378:metabolism* / Q000633:toxicity; D014874:Water Pollutants, Chemical / Q000737:chemistry / Q000378:metabolism* / Q000633:toxicity
D004967:Estrogens; D005026:Ethylene Glycols; D014874:Water Pollutants, Chemical; C021754:terics; D008031:Lignin