Effect of Staphylococcus aureus and Streptococcus uberis on apoptosis of bovine mammary gland lymphocytes.
The aim of this study was to determine whether lymphocyte apoptosis is modulated by infections caused by Staphylococcus aureus and Streptococcus uberis. Samples of cell populations were obtained by lavage of the mammary glands at 4 intervals (24, 48, 72 and 168 h) following infection. The percentage of apoptotic lymphocytes peaked at 168 h after challenge with S. aureus or S. uberis. Subsequent experiments focused on in vitro cultivation of mammary gland lymphocytes with S. aureus and S. uberis. These experiments showed a lower percentage of apoptotic lymphocytes following 3h of cultivating cells with bacteria than after cultivation without bacteria. The results demonstrate that during both experimental infection of bovine mammary glands with S. aureus or S. uberis and during in vitro cultivation of lymphocytes with S. aureus or S. uberis, apoptosis of lymphocytes is delayed.
Slama
Petr
P
Department of Animal Morphology, Mendel University of Agriculture and Forestry in Brno, 613 00 Brno, Czech Republic. xslama@node.mendelu.cz
Sladek
Zbysek
Z
Rysanek
Dusan
D
Langrova
Tereza
T
D000818:Animals; D000954:Antigens, Surface / Q000032:analysis; D017209:Apoptosis; D018801:CD2 Antigens / Q000032:analysis; D002417:Cattle; D002418:Cattle Diseases / Q000276:immunology / Q000382:microbiology; D005260:Female; D007074:Immunoglobulin G / Q000032:analysis; D008214:Lymphocytes / Q000166:cytology / Q000276:immunology* / Q000502:physiology; D008321:Mammary Glands, Animal / Q000166:cytology / Q000382:microbiology* / Q000502:physiology; D013203:Staphylococcal Infections / Q000276:immunology / Q000662:veterinary*; D013211:Staphylococcus aureus / Q000302:isolation & purification*; D013290:Streptococcal Infections / Q000276:immunology / Q000662:veterinary*; D013291:Streptococcus / Q000302:isolation & purification*
D000954:Antigens, Surface; D018801:CD2 Antigens; D007074:Immunoglobulin G