Electroformation of giant vesicles from an inverse phase precursor.
We discuss a simple modification of the well-known method of giant vesicle electroformation that allows for a direct addition of water-soluble species to the phospholipid bilayers. Using this modified method, we prepare phospholipid vesicles decorated with chitosan, a water-soluble polysaccharide currently investigated for potential pharmacological applications. We find that the method allows this polysaccharide with primary amino groups on every glucose subunit to be tightly bound to the membrane, rather than simply being encapsulated.
Mertins
Omar
O
Institut Charles Sadron, UPR22, Centre National de la Recherche Scientifique, Université de Strasbourg, Strasbourg, France. omar@iq.ufrgs.br
da Silveira
Nádya P
NP
Pohlmann
Adriana R
AR
Schröder
André P
AP
Marques
Carlos M
CM
D002138:Calibration; D048271:Chitosan / Q000032:analysis / Q000737:chemistry; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000737:chemistry; D008051:Lipid Bilayers / Q000737:chemistry; D008856:Microscopy, Fluorescence; D010743:Phospholipids / Q000737:chemistry; D053835:Unilamellar Liposomes / Q000737:chemistry*
D005456:Fluorescent Dyes; D008051:Lipid Bilayers; D010743:Phospholipids; D053835:Unilamellar Liposomes; D048271:Chitosan