The value of leukocyte adhesion molecules in patients after ischemic stroke.
Leukocyte recruitment and inflammatory response play an important patho-physiologic role after cerebral ischemia. This study aimed to evaluate whether leukocyte adhesion molecules can predict clinical outcome in patients after ischemic stroke. We prospectively examined serial changes in p-selectin glycoprotein ligand-1 (PSGL-1), macrophage antigen-1 (Mac-1), and lymphocyte function-associated antigen-1 (LFA-1) expression by leukocyte subsets using flow cytometry at various time points in 65 acute ischemic stroke patients and 60 controls. PSGL-1 expression on neutrophils and monocytes was significantly higher from day 1 to 90 after stroke as compared with control subjects (p < 0.05). The expression of monocyte Mac-1, LFA-1, and neutrophil Mac-1 were also significantly increased on days 1 and 7 after stroke than in control subjects (p < 0.05). Neutrophil PSGL-1 expression on day 1 was significantly higher in patients with early neurologic deterioration (END) (p < 0.01). Monocyte Mac-1 expression positively correlated with National Institutes of Health Stroke Scale (NIHSS) scores on admission (p = 0.013, gamma = 0.318). Underlying disease of diabetes mellitus and NIHSS score on admission were independently associated with 3-month outcome. The expressions of leukocyte adhesion molecules on admission are significantly increased in patients with acute ischemic stroke. This study shows that higher neutrophil PSGL-1 expression on admission may imply a higher risk for END and that monocyte Mac-1 expression on admission reflects the severity of ischemic stroke on admission.
Tsai
Nai-Wen
NW
Department of Neurology, Chang Gung Memorial Hospital, Kaohsiung Medical Center, Chang Gung University College of Medicine, 123, Ta Pei Road, Niao Sung Hsiang, Kaohsiung Hsien 833, Taiwan, ROC.
Chang
Wen-Neng
WN
Shaw
Chen-Fu
CF
Jan
Chung-Ren
CR
Huang
Chi-Ren
CR
Chen
Shang-Der
SD
Chuang
Yao-Chung
YC
Lee
Lian-Hui
LH
Lu
Cheng-Hsien
CH
D000368:Aged; D015415:Biomarkers / Q000032:analysis / Q000378:metabolism; D002545:Brain Ischemia / Q000150:complications* / Q000276:immunology* / Q000503:physiopathology; D002448:Cell Adhesion / Q000276:immunology; D015815:Cell Adhesion Molecules / Q000032:analysis / Q000378:metabolism*; D002634:Chemotaxis, Leukocyte / Q000276:immunology*; D018450:Disease Progression; D004660:Encephalitis / Q000150:complications* / Q000276:immunology* / Q000503:physiopathology; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D007962:Leukocytes / Q000166:cytology / Q000378:metabolism; D016169:Lymphocyte Function-Associated Antigen-1 / Q000032:analysis / Q000378:metabolism; D016177:Macrophage-1 Antigen / Q000032:analysis / Q000378:metabolism; D008297:Male; D008562:Membrane Glycoproteins / Q000032:analysis / Q000378:metabolism; D008875:Middle Aged; D011237:Predictive Value of Tests; D011379:Prognosis; D011446:Prospective Studies; D020521:Stroke / Q000150:complications / Q000276:immunology / Q000503:physiopathology; D015854:Up-Regulation / Q000276:immunology
D015415:Biomarkers; D015815:Cell Adhesion Molecules; D016169:Lymphocyte Function-Associated Antigen-1; D016177:Macrophage-1 Antigen; D008562:Membrane Glycoproteins; C084882:P-selectin ligand protein