Genetic evidence for the requirement of the endocytic pathway in the uptake of coenzyme Q6 in Saccharomyces cerevisiae.
Coenzyme Q is an isoprenylated benzoquinone lipid that functions in respiratory electron transport and as a lipid antioxidant. Dietary supplementation with Q is increasingly used as a therapeutic for treatment of mitochondrial and neurodegenerative diseases, yet little is known regarding the mechanism of its uptake. As opposed to other yeast backgrounds, EG103 strains are unable to import exogenous Q(6) to the mitochondria. Furthermore, the distribution of exogenous Q(6) among endomembranes suggests an impairment of the membrane traffic at the level of the endocytic pathway. This fact was confirmed after the detection of defects in the incorporation of FM4-64 marker and CPY delivery to the vacuole. A similar effect was demonstrated in double mutant strains in Q(6) synthesis and several steps of endocytic process; those cells are unable to uptake exogenous Q(6) to the mitochondria and restore the growth on non-fermentable carbon sources. Additional data about the positive effect of peptone presence for exogenous Q(6) uptake support the hypothesis that Q(6) is transported to mitochondria through an endocytic-based system.
Centro Andaluz de Biología del Desarrollo, Universidad Pablo de Olavide-CSIC and Centre for Biomedical Research on Rare Diseases (CIBERER), ISCIII, E-41013 Sevilla, Spain.
Sergio
S
Padilla-López
María
M
Jiménez-Hidalgo
Alejandro
A
Martín-Montalvo
Catherine F
CF
Clarke
Plácido
P
Navas
Carlos
C
Santos-Ocaña
D002462:Cell Membrane / Q000235:genetics / Q000502:physiology; D017931:DNA Primers; D004705:Endocytosis / Q000502:physiology*; D006056:Golgi Apparatus / Q000502:physiology; D008928:Mitochondria / Q000235:genetics / Q000502:physiology; D009154:Mutation; D016133:Polymerase Chain Reaction; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000502:physiology*; D014451:Ubiquinone / Q000235:genetics / Q000502:physiology*; D014617:Vacuoles / Q000235:genetics / Q000502:physiology
D017931:DNA Primers; C032165:ubiquinone 6; D014451:Ubiquinone