PEG-based thermogels: applicability in physiological media.
Novel biocompatible thermogels have been synthesized and characterized. The hydrogelators were synthesized by atom transfer radical copolymerization of 2-(2-methoxyethoxy)ethyl methacrylate (MEO(2)MA) and oligo(ethylene glycol) methyl ether methacrylate (OEGMA(475), M(n)=475 g mol(-1) or OEGMA(300), M(n)=300 g mol(-1)) in the presence of a 4-arm star poly(ethylene glycol) (PEG) macroinitiator. The formed macromolecules possess a permanently hydrophilic PEG core and thermoresponsive P(MEO(2)MA-co-OEGMA) outer-blocks. These star-block architectures exhibit an inverse thermogelation behavior in aqueous medium. Typically, above their lower critical solution temperature (LCST), the thermoresponsive P(MEO(2)MA-co-OEGMA) precipitate, thus forming physical crosslinks, which are stabilized in water by hydrophilic PEG bridges. This thermo-induced sol-gel transition can be adjusted within a near-physiological range of temperature by simply varying the composition of the thermoresponsive segments. Moreover, these novel hydrogelators formed free-standing gels in various buffer solutions (e.g., PBS, Tris, MOPS, bicine and HEPES) and in cell culture media. In saline solutions, a weak salting-out effect was observed. However, other components of physiological media (e.g., buffering agents, amino acids, vitamins, proteins) did not hinder the thermogelation process. Hence, these novel thermogels appear as highly attractive candidates for applications in biosciences.
Research Group Nanotechnology for Life Science, Fraunhofer Institute for Applied Polymer Research, Geiselbergstrasse 69, Potsdam 14476, Germany.
Nezha
N
Badi
Jean-François
JF
Lutz
D001672:Biocompatible Materials; D002021:Buffers; D002850:Chromatography, Gel; D003470:Culture Media; D015195:Drug Design; D004563:Electrochemistry; D005782:Gels / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D011092:Polyethylene Glycols / Q000737:chemistry*; D012995:Solubility; D013816:Thermodynamics; D014783:Viscosity
D001672:Biocompatible Materials; D002021:Buffers; D003470:Culture Media; D005782:Gels; D011092:Polyethylene Glycols