[Basic experimental and clinical research on peritoneal dialysis in the past 16 years].
To summarized the experiences from our basic experimental and clinical research on peritoneal dialysis. In the past 16 years, peritoneal fibrosis rat models and rabbit models of peritonitis were first established successfully in our laboratory in China. Peritoneal mesothelial cells were also separated and identificated. Besides, we assessed the biocompatibility of peritoneal dialysis fluid and analyzed the molecular mechanism of peritoneal mesothelial cell injury. We demonstrated the key role of transforming growth factor-beta1 (TGF-beta1), connective tissue growth factor (CTGF) and peroxisome proliferative activated receptor-gamma (PPAR-gamma) in the pathogenesis of peritoneal fibrosis, as well as their regulation of molecular mechanism. Furthermore, we transfected the plasmids encoding TGF-beta1-shRNA or pCTGF-shRNA into peritoneal cells and tissues by nanocarrier technologies. In clinical research, the positioning of peritoneal dialysis catheters, peritoneal dialysis treatment modalities and the prevention and treatment of its complications were studied. The characteristics and mechanism of solute transport in peritoneal dialysis was also explored.
Department of Nephrology, Second Xiangya Hospital, Central South University, Changsha 410011, China. lfy410@yahoo.com.cn
Fuyou
F
Liu
Youming
Y
Peng
Shalin
S
Zou
Guanghui
G
Ling
Jing
J
Nie
Wenbin
W
Tang
Xun
X
Zhou
Shaobin
S
Duan
Jun
J
Li
Yinghong
Y
Liu
Hong
H
Liu
Fang
F
Yuan
Li
L
Xiao
Li
L
Zhuo
Junxiang
J
Chen
Xing
X
Chen
Meichu
M
Cheng
Jianling
J
Zhu
Xiaoping
X
Zhu
Ji' an
Ja
Luo
Min
M
Fan
Hao
H
Zhang
Lin
L
Sun
D000818:Animals; D055513:Connective Tissue Growth Factor / Q000378:metabolism; D005355:Fibrosis / Q000503:physiopathology / Q000517:prevention & control; D006801:Humans; D007676:Kidney Failure, Chronic / Q000378:metabolism / Q000628:therapy*; D010530:Peritoneal Dialysis / Q000379:methods*; D010531:Peritoneal Dialysis, Continuous Ambulatory / Q000009:adverse effects; D010537:Peritoneum / Q000473:pathology*; D011817:Rabbits; D051381:Rats; D012189:Retrospective Studies; D000267:Tissue Adhesions / Q000503:physiopathology / Q000517:prevention & control; D016212:Transforming Growth Factor beta / Q000378:metabolism
C526317:CCN2 protein, human; D016212:Transforming Growth Factor beta; D055513:Connective Tissue Growth Factor