Heme d1 nitrosyl complex of cd1 nitrite reductase studied by high-field-pulse electron paramagnetic resonance spectroscopy.
W-band (95 GHz) HYSCORE and pulse ENDOR are used to characterize the nitrosyl d(1) heme complex (d(1)NO) of cd(1) nitrite reductase from Pseudomonas aeruginosa in the wild type and the Y10F mutant. The spectra and the derived (14)N hyperfine and quadrupole interactions were found to be the same for wt and Y10F. This suggests that Tyr10 does not influence the NO ligand orientation in the reduced state in solution. This study is the first application of HYSCORE at high fields and shows its potential for characterizing low gamma nuclei with large hyperfine couplings.
Radoul
Marina
M
Department of Chemical Physics, Weizmann Institute of Science, Rehovot 76100, Israel.
Centola
Fabio
F
Rinaldo
Serena
S
Cutruzzolà
Francesca
F
Pecht
Israel
I
Goldfarb
Daniella
D
D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D003460:Crystallization; D003580:Cytochromes / Q000737:chemistry* / Q000235:genetics* / Q000378:metabolism; D004578:Electron Spin Resonance Spectroscopy; D006418:Heme / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism; D008024:Ligands; D009569:Nitric Oxide / Q000737:chemistry* / Q000378:metabolism; D009572:Nitrite Reductases / Q000737:chemistry* / Q000235:genetics* / Q000378:metabolism; D011485:Protein Binding; D011487:Protein Conformation; D011550:Pseudomonas aeruginosa / Q000201:enzymology* / Q000235:genetics; D014443:Tyrosine / Q000737:chemistry
D001426:Bacterial Proteins; D003580:Cytochromes; D008024:Ligands; D009569:Nitric Oxide; D014443:Tyrosine; D006418:Heme; C029400:heme d1; D009572:Nitrite Reductases; C028123:nitrite reductase (NO-forming)