Direct automatic determination of free and total anesthetic drugs in human plasma by use of a dual (microdialysis-microextraction by packed sorbent) sample treatment coupled at-line to NACE-MS.
This paper reports for the first time the use of microextraction by packed sorbent in combination with CE. The combined system was used to determine anesthetic drugs in human plasma. A microdialysis fiber was coupled on-line to the microextraction unit in order to distinguish between free and total concentrations of drugs. The system was automated by connecting the microextraction unit to a syringe pump and interfacing it to a computer. The ensuing method allows the determination of 10 microg/L concentrations of free drugs and 1 microg/L concentrations of total drugs from only 200 microL of sample with an RSD of less than 9%.
Morales-Cid
Gabriel
G
Department of Analytical Chemistry, Campus de Rabanales, University of Cordoba, Cordoba, Spain.
Cárdenas
Soledad
S
Simonet
Bartolomé M
BM
Valcárcel
Miguel
M
D000777:Anesthetics / Q000097:blood* / Q000302:isolation & purification; D019075:Electrophoresis, Capillary / Q000379:methods*; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods*; D017551:Microdialysis / Q000379:methods*; D052616:Solid Phase Extraction / Q000379:methods*
D000777:Anesthetics