Chromatin conformation signatures of cellular differentiation.
One of the major genomics challenges is to better understand how correct gene expression is orchestrated. Recent studies have shown how spatial chromatin organization is critical in the regulation of gene expression. Here, we developed a suite of computer programs to identify chromatin conformation signatures with 5C technology http://Dostielab.biochem.mcgill.ca. We identified dynamic HoxA cluster chromatin conformation signatures associated with cellular differentiation. Genome-wide chromatin conformation signature identification might uniquely identify disease-associated states and represent an entirely novel class of human disease biomarkers.
Fraser
James
J
Department of Biochemistry and McGill Cancer Center, McGill University, 3655 Promenade Sir-William-Osler, Montréal, H3G1Y6, Canada.
Rousseau
Mathieu
M
Shenker
Solomon
S
Ferraiuolo
Maria A
MA
Hayashizaki
Yoshihide
Y
Blanchette
Mathieu
M
Dostie
Josée
J
D002454:Cell Differentiation / Q000187:drug effects / Q000235:genetics / Q000502:physiology*; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D002843:Chromatin / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D042002:Chromatin Assembly and Disassembly; D003198:Computer Simulation; D015870:Gene Expression / Q000187:drug effects; D015723:Gene Library; D018398:Homeodomain Proteins / Q000235:genetics; D006801:Humans; D008954:Models, Biological; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012984:Software; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D002843:Chromatin; D018398:Homeodomain Proteins; C088144:HoxA protein; D013755:Tetradecanoylphorbol Acetate