Fos expression and hormone changes following electrical stimulation of the posterodorsal amygdala and the effects on food intake in conscious female rats.
Previous studies have shown that lesions of the posterodorsal amygdala (PDA) produce hyperphagia and obesity in female rats. To better understand the role of the PDA in the regulation of feeding behavior, the current study examined the effects of electrical stimulation of the PDA of female rats on food intake and identified neurons activated by PDA stimulation using Fos immunohistochemistry. Hormonal levels following stimulation of the PDA were also investigated. Electrical stimulation (100 muA, 0.2 ms, 20 Hz, 10 min) of the PDA remarkably decreased 1 h food intake of rats. Following PDA stimulation, the number of Fos-like immunoreactive neurons increased in the caudal and intermediate parts of the nucleus of the solitary tract (NST), the area postrema (AP), the external lateral subnucleus of the parabrachial nucleus (PBN), the arcuate nucleus of the hypothalamus (Arc), and the central amygdaloid nucleus (CeA). The level of circulating leptin was elevated significantly by PDA stimulation as well. Together with previous studies, the results suggest that the PDA may play an important role in the regulation of feeding behavior, at least partly by modulating the circulating leptin, and that the caudal and intermediate parts of the NST, AP, external lateral PBN, Arc, and CeA probably participate in this regulation.
Yang
Xuejuan
X
Department of Physiology and Pathophysiology, Key Laboratory of Environment and Genes Related to Diseases, Ministry of Education, Xi'an Jiaotong University School of Medicine, Xi'an, Shaanxi 710061, China.
Yan
Jianqun
J
Lu
Bo
B
Zhao
Xiaolin
X
Lei
Qi
Q
Yang
Dejun
D
Chen
Ke
K
Zhao
Shiru
S
Zhu
Guangjing
G
D000679:Amygdala / Q000166:cytology / Q000378:metabolism*; D000818:Animals; D001069:Appetite Regulation / Q000502:physiology*; D001111:Arcuate Nucleus of Hypothalamus / Q000166:cytology / Q000378:metabolism; D031608:Area Postrema / Q000166:cytology / Q000378:metabolism; D017776:Autonomic Pathways / Q000166:cytology / Q000378:metabolism*; D001921:Brain / Q000166:cytology / Q000378:metabolism*; D003243:Consciousness; D004558:Electric Stimulation; D005260:Female; D007150:Immunohistochemistry; D020738:Leptin / Q000378:metabolism*; D016760:Proto-Oncogene Proteins c-fos / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D017552:Solitary Nucleus / Q000166:cytology / Q000378:metabolism; D015854:Up-Regulation / Q000502:physiology
D020738:Leptin; D016760:Proto-Oncogene Proteins c-fos