N-acetylcysteine prevents deleterious effects of ischemia/reperfusion injury on healing of colonic anastomosis in rats.
This study was designed to determine the effects of intraperitoneally or orally administered N-acetylcysteine (NAC) on wound healing following resection and anastomosis of a colon segment with ischemia/reperfusion injury. Forty female Sprague-Dawley rats were randomly allocated to one of four groups containing 10 rats each: (1) normal resection plus anastomosis; (2) ischemia/reperfusion plus resection plus anastomosis; (3) ischemia/reperfusion plus resection plus anastomosis plus intraperitoneal NAC; (4) ischemia/reperfusion plus resection plus anastomosis plus oral NAC. Group comparison showed that the anastomosis bursting pressure was significantly higher in group 3 than in the other groups. The mean tissue hydroxyproline concentration in the anastomotic tissue was significantly lower in group 2 than in the other groups. The collagen deposition was significantly increased on day 7 in groups 3 and 4 compared to the other groups. In conclusion, this study demonstrates that NAC significantly prevents the effects of reperfusion injury on colonic anastomoses in a rat model.
Kabali
B
B
Department of General Surgery, Dicle University, Medical School, Diyarbakir, Turkey.
Girgin
S
S
Gedik
E
E
Ozturk
H
H
Kale
E
E
Buyukbayram
H
H
D000111:Acetylcysteine / Q000008:administration & dosage*; D000284:Administration, Oral; D000714:Anastomosis, Surgical / Q000009:adverse effects; D000818:Animals; D003106:Colon / Q000601:surgery*; D005260:Female; D016166:Free Radical Scavengers / Q000008:administration & dosage*; D007263:Infusions, Parenteral; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000517:prevention & control*; D014945:Wound Healing / Q000187:drug effects*
D016166:Free Radical Scavengers; D000111:Acetylcysteine