GC-1 mRHBDD1 knockdown spermatogonia cells lose their spermatogenic capacity in mouse seminiferous tubules.
BACKGROUND
Apoptosis is important for regulating spermatogenesis. The protein mRHBDD1 (mouse homolog of human RHBDD1)/rRHBDD1 (rat homolog of human RHBDD1) is highly expressed in the testis and is involved in apoptosis of spermatogonia. GC-1, a spermatogonia cell line, has the capacity to differentiate into spermatids within the seminiferous tubules. We constructed mRHBDD1 knockdown GC-1 cells and evaluated their capacity to differentiate into spermatids in mouse seminiferous tubules.


RESULTS
Stable mRHBDD1 knockdown GC-1 cells were sensitive to apoptotic stimuli, PS341 and UV irradiation. In vitro, they survived and proliferated normally. However, they lost the ability to survive and differentiate in mouse seminiferous tubules.


CONCLUSIONS
Our findings suggest that mRHBDD1 may be associated with mammalian spermatogenesis.
State Key Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, Peking Union Medical College, Tsinghua University 5 Dong Dan San Tiao, Beijing 100005, PR China. wang.linfang@imicams.ac.cn
Yong
Y
Wang
Wei
W
Song
Shuchun
S
Li
Xin
X
Guan
Shiying
S
Miao
Shudong
S
Zong
S S
SS
Koide
Linfang
L
Wang
D000818:Animals; D017209:Apoptosis / Q000187:drug effects / Q000528:radiation effects; D015153:Blotting, Western; D001897:Boronic Acids / Q000494:pharmacology; D000069286:Bortezomib; D002460:Cell Line; D049109:Cell Proliferation; D066246:ErbB Receptors / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D005434:Flow Cytometry; D020869:Gene Expression Profiling; D055785:Gene Knockdown Techniques; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D008297:Male; D008565:Membrane Proteins; D051379:Mice; D008807:Mice, Inbred BALB C; D011719:Pyrazines / Q000494:pharmacology; D034741:RNA, Small Interfering / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D012038:Regeneration; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012671:Seminiferous Tubules / Q000166:cytology / Q000502:physiology* / Q000601:surgery; D013091:Spermatogenesis / Q000235:genetics / Q000502:physiology*; D013093:Spermatogonia / Q000166:cytology* / Q000378:metabolism / Q000637:transplantation; D013737:Testis / Q000166:cytology / Q000378:metabolism; D014162:Transfection; D014466:Ultraviolet Rays
D001897:Boronic Acids; D008565:Membrane Proteins; D011719:Pyrazines; D034741:RNA, Small Interfering; D011993:Recombinant Fusion Proteins; C484141:Rhbdf1 protein, mouse; D049452:Green Fluorescent Proteins; D000069286:Bortezomib; D066246:ErbB Receptors