Transient and stable transgenesis using tol2 transposon vectors.
Transgenesis is an important methodology for studying the function of genes and genomes in model plants and animals. For the model vertebrate zebrafish, methods using the Tol2 transposable element have been developed for this purpose. With these methods, the function of the transgene can be analyzed in both transient and stable transgenic fish. Recently, cis-sequences necessary for transposition of the Tol2 element were revealed. This enabled development of transposon vectors containing minimal DNA sequences that are easily manipulated. More recently, several transposon vectors containing the Gateway sequence were created and reported. These are useful because any foreign sequences can be cloned into a transposon vector fairly easily and rapidly. This chapter describes the features of these transposon vectors, and protocols to perform transgenesis in zebrafish.
Division of Molecular and Developmental Biology, National Institute of Genetics, 1111 Yata, Mishima, Shizuoka, 411-8540, Japan.
Hiroshi
H
Kikuta
Koichi
K
Kawakami
D000818:Animals; D030801:Animals, Genetically Modified; D004247:DNA / Q000235:genetics / Q000378:metabolism; D004251:DNA Transposable Elements; D015870:Gene Expression; D018014:Gene Transfer Techniques; D017930:Genes, Reporter; D005818:Genetic Engineering / Q000379:methods; D005822:Genetic Vectors / Q000235:genetics / Q000378:metabolism; D018095:Germ-Line Mutation; D049452:Green Fluorescent Proteins; D015027:Zebrafish / Q000196:embryology / Q000235:genetics* / Q000378:metabolism
D004251:DNA Transposable Elements; D049452:Green Fluorescent Proteins; D004247:DNA