First gene cassettes of integrons as targets in finding adaptive genes in metagenomes.
The first gene cassettes of integrons are involved in the last adaptation response to changing conditions and are also the most expressed. We propose a rapid method for the selection of clones carrying an integron first gene cassette that is useful for finding adaptive genes in environmental metagenomic libraries.
Huang
Lionel
L
Université de Pau et des Pays de l'Adour, IPREM UMR CNRS, IBEAS, BP, Pau, France.
Cagnon
Christine
C
Caumette
Pierre
P
Duran
Robert
R
D000220:Adaptation, Biological; D001419:Bacteria / Q000235:genetics*; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D032023:Integrons; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA
D004269:DNA, Bacterial