Delayed stress-induced differences in locomotor and depression-related behaviour in female neuropeptide-Y Y1 receptor knockout mice.
Neuropeptide-Y acting through Y1 receptors reduces anxiety and stress sensitivity in rodents. In Y1 receptor knockout (Y1⁻/⁻) mice, however, anxiety-related behaviour is altered only in a context-dependent manner. Here, we investigated whether stress causes a delayed change in the emotional-affective behaviour of female Y1⁻/⁻ mice. Locomotor and anxiety-related behaviour was assessed with the elevated plus-maze (EPM) test and depression-like behaviour with the forced swim test (FST). These behavioural tests were also used as experimental stress paradigms. Locomotion and anxiety-like behaviour did not differ between naïve control and Y1⁻/⁻ mice. One week after the FST, locomotion was reduced in control animals but unchanged in Y1⁻/⁻ mice, whereas anxiety-like behaviour remained unaltered in both genotypes. Depression-like behaviour (immobility) was identical in naïve control and Y1⁻/⁻ mice but, 1 week after the EPM test, was attenuated in Y1⁻/⁻ mice relative to control animals. Our data show that naïve female Y1⁻/⁻ mice do not grossly differ from female control animals in their locomotor and depression-like behaviour. Exposure to the stress associated with behavioural testing, however, leads to delayed genotype-dependent differences in locomotion and depression-like behaviour. These findings attest to a role of Y1 receptor signalling in the control of stress coping and/or adaptation.
Research Unit of Translational Neurogastroenterology, Institute of Experimental and Clinical Pharmacology, Medical University of Graz, Graz, Austria.
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Painsipp
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Sperk
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Herzog
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Holzer
D000698:Analgesia; D000818:Animals; D001007:Anxiety; D001522:Behavior, Animal; D003345:Corticosterone / Q000097:blood; D003863:Depression / Q000097:blood / Q000503:physiopathology* / Q000523:psychology; D005260:Female; D051379:Mice; D057507:Mice, 129 Strain; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D009043:Motor Activity; D009478:Neuropeptide Y / Q000502:physiology*; D010147:Pain Measurement; D011930:Reaction Time; D017476:Receptors, Neuropeptide Y / Q000235:genetics / Q000502:physiology*; D015398:Signal Transduction; D013312:Stress, Physiological; D013315:Stress, Psychological / Q000097:blood; D013997:Time Factors
D009478:Neuropeptide Y; C507698:Npy1r protein, mouse; D017476:Receptors, Neuropeptide Y; D003345:Corticosterone