The promotion of human mesenchymal stem cell proliferation by superparamagnetic iron oxide nanoparticles.
Superparamagnetic iron oxide (SPIO) nanoparticles are very useful in cell imaging; meanwhile, however, biosafety concerns associated with their use, especially on therapeutic stem cells, have arisen. Most studies of biosafety issues focus on whether the nanoparticles have deleterious effects. Here, we report that Ferucarbotran, an ionic SPIO, is not toxic to human mesenchymal stem cells (hMSCs) under the conditions of these experiments but instead increases cell growth. Ferucarbotran-promoted cell growth is due to its ability to diminish intracellular H2O2 through intrinsic peroxidase-like activity. Also, Ferucarbotran can accelerate cell cycle progression, which may be mediated by the free iron (Fe) released from lysosomal degradation and involves the alteration of Fe on the expression of the protein regulators of the cell cycle.
Huang
Dong-Ming
DM
Center for Nanomedicine Research, National Health Research Institutes, 35 Keyan Road, Zhunan, Miaoli 350, Taiwan. dmhuang@nhri.org.tw
Hsiao
Jong-Kai
JK
Chen
Ying-Chun
YC
Chien
Li-Ying
LY
Yao
Ming
M
Chen
Yin-Kai
YK
Ko
Bor-Sheng
BS
Hsu
Szu-Chun
SC
Tai
Lin-Ai
LA
Cheng
Hui-Ying
HY
Wang
Shih-Wei
SW
Yang
Chung-Shi
CS
Chen
Yao-Chang
YC
D000818:Animals; D002453:Cell Cycle / Q000502:physiology; D049109:Cell Proliferation / Q000187:drug effects*; D003287:Contrast Media / Q000737:chemistry / Q000494:pharmacology; D003911:Dextrans; D005290:Ferric Compounds / Q000737:chemistry / Q000494:pharmacology*; D052203:Ferrosoferric Oxide; D006801:Humans; D006861:Hydrogen Peroxide / Q000378:metabolism; D007501:Iron / Q000737:chemistry / Q000494:pharmacology*; D008279:Magnetic Resonance Imaging; D008280:Magnetics; D058185:Magnetite Nanoparticles; D008422:Materials Testing; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000187:drug effects* / Q000502:physiology*; D053758:Nanoparticles / Q000737:chemistry*; D016877:Oxidants / Q000378:metabolism; D010084:Oxidation-Reduction; D010087:Oxides / Q000737:chemistry / Q000494:pharmacology*; D010544:Peroxidases / Q000378:metabolism
D003287:Contrast Media; D003911:Dextrans; D005290:Ferric Compounds; D058185:Magnetite Nanoparticles; D016877:Oxidants; D010087:Oxides; C000499:ferric oxide; D006861:Hydrogen Peroxide; D007501:Iron; D010544:Peroxidases; C065507:ferumoxides; D052203:Ferrosoferric Oxide