Two-dimensional liquid chromatography analysis of synthetic polymers using fast size exclusion chromatography at high column temperature.
In recent years, two-dimensional liquid chromatography (2D-LC) has been used increasingly for the analysis of synthetic polymers. A 2D-LC analysis provides richer information than a single chromatography analysis at the cost of longer analysis time. The time required for a comprehensive 2D-LC analysis is essentially proportional to the analysis time of the second dimension separation. Many of 2D-LC analyses of synthetic polymers have employed size exclusion chromatography (SEC) for the second-dimension analysis due to the relatively short analysis time in addition to the wide use in the polymer analysis. Nonetheless, short SEC columns are often used for 2D-LC analyses to reduce the separation time, which inevitably deteriorates the resolution. In this study, we demonstrated that high temperature SEC can be employed as an efficient second-LC in the 2D-LC separation of synthetic polymers. By virtue of high temperature operation (low solvent viscosity and high diffusivity of the polymer molecules), a normal length SEC column can be used at high flow rate with little loss in resolution.
Department of Chemistry and BK 21 School of Molecular Science, Pohang University of Science and Technology, Nam-Gu, Pohang, South Korea.
Kyuhyun
K
Im
Hae-Woong
HW
Park
Sekyung
S
Lee
Taihyun
T
Chang
D002850:Chromatography, Gel / Q000379:methods*; D002853:Chromatography, Liquid / Q000379:methods*; D006358:Hot Temperature; D011108:Polymers / Q000032:analysis*; D013056:Spectrophotometry, Ultraviolet
D011108:Polymers