Isolation and characterization of a novel chondroitin sulfate from squid liver integument rich in N-acetylgalactosamine(4,6-disulfate) and glucuronate(3-sulfate) residues.
Novel chondroitin sulfate (CS) chains with an average molecular mass of 79.6 kDa were purified from squid liver integument. A compositional analysis of the CS chains using chondroitinases (CSases) ABC and AC-I revealed a range of variably sulfated disaccharides with GlcAbeta1-->3GalNAc(6-sulfate), GlcAbeta1-->3GalNAc(4-sulfate), and GlcAbeta1-->3GalNAc(4,6-disulfate) as the major ones, significant amounts of rare 3-sulfated GlcA-containing disaccharides, and a small amount of nonsulfated GlcAbeta1-->3GalNAc. The CS chains exhibited neurite outgrowth-promoting activity toward embryonic mouse hippocampal neurons, which was abolished completely by digestion with CSase ABC or AC-I. Consequently, whether these CS chains interact with heparin-binding growth factors was tested in a BIAcore system. All of the growth factors exhibited concentration-dependent and specific binding. CS chains from squid liver integument, with their unique composition and strong biological activities, may be a good candidate for therapeutic application.
Laboratory of Proteoglycan Signaling and Therapeutics, Hokkaido University, Graduate School of Life Science, Kita-ku, Sapporo 001-0021, Japan.
Ajaya Kumar
AK
Shetty
Takanari
T
Kobayashi
Shuji
S
Mizumoto
Masaki
M
Narumi
Yoshiaki
Y
Kudo
Shuhei
S
Yamada
Kazuyuki
K
Sugahara
D000116:Acetylgalactosamine / Q000737:chemistry*; D000818:Animals; D002809:Chondroitin Sulfates / Q000737:chemistry* / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid; D049832:Decapodiformes / Q000378:metabolism*; D005965:Glucuronates / Q000737:chemistry*; D008099:Liver / Q000378:metabolism*; D015394:Molecular Structure; D020349:Surface Plasmon Resonance
D005965:Glucuronates; D002809:Chondroitin Sulfates; D000116:Acetylgalactosamine