The effect of calcitonin gene-related peptide on healing of intestinal anastomosis in rats with experimental obstructive jaundice.
OBJECTIVE
Intestinal anastomotic healing is a complex procedure in which several mediators and cytokines play roles. Calcitonin gene-related peptide is an important neuropeptide in inflammation. In this study we aimed to investigate the effect of calcitonin gene-related peptide on healing of intestinal anastomosis in rats with obstructive jaundice.


METHODS
Obstructive jaundice was induced in rats by the ligation and division of the common bile duct. Four days after the operation, intestinal anastomosis was performed, and either calcitonin gene-related peptide or 0.9% NaCl was administered intraperitoneally to these jaundiced rats and controls. The concentrations of serum tumor necrosis factor-alpha (TNF-alpha) and triglyceride levels of all rats were measured, and healing of the anastomosis was evaluated by measuring the bursting pressure and hydroxyproline content on the 7th postoperative day.


RESULTS
Calcitonin gene-related peptide was found to have positive effects on healing of the anastomosis by inhibiting the effects of TNF-alpha and increasing the bursting pressure and hydroxyproline content of the anastomosis.


CONCLUSIONS
Calcitonin gene-related peptide increases anastomotic wound healing in experimental anastomosis in the presence of obstructive jaundice in rats.
Department of General Surgery, Faculty of Medicine, Gazi University, Ankara, Turkey.
Sezai
S
Leventoglu
Hande
H
Koksal
Banu
B
Sancak
Ferit
F
Taneri
Erhan
E
Onuk
D000714:Anastomosis, Surgical; D000818:Animals; D015740:Calcitonin Gene-Related Peptide / Q000494:pharmacology*; D004797:Enzyme-Linked Immunosorbent Assay; D006909:Hydroxyproline / Q000032:analysis; D007422:Intestines / Q000601:surgery; D041781:Jaundice, Obstructive / Q000188:drug therapy*; D008297:Male; D011312:Pressure; D051381:Rats; D017208:Rats, Wistar; D014280:Triglycerides / Q000097:blood; D014409:Tumor Necrosis Factor-alpha / Q000097:blood / Q000187:drug effects; D014665:Vasodilator Agents / Q000494:pharmacology*; D014945:Wound Healing / Q000187:drug effects* / Q000502:physiology
D014280:Triglycerides; D014409:Tumor Necrosis Factor-alpha; D014665:Vasodilator Agents; D015740:Calcitonin Gene-Related Peptide; D006909:Hydroxyproline