Transient transformation of plants.
Transient expression in plants is a valuable tool for many aspects of functional genomics and promoter testing. It can be used both to over-express and to silence candidate genes. It is also scaleable and provides a viable alternative to microbial fermentation and animal cell culture for the production of recombinant proteins. It does not depend on chromosomal integration of heterologous DNA so is a relatively facile procedure and can lead to high levels of transgene expression. Recombinant DNA can be introduced into plant cells via physical methods, via Agrobacterium or via viral vectors.
Jones
Huw D
HD
CPI Division, Rothamsted Research, Harpenden, Hertfordshire, UK.
Doherty
Angela
A
Sparks
Caroline A
CA
D016960:Agrobacterium tumefaciens / Q000235:genetics; D019470:Biolistics; D004269:DNA, Bacterial / Q000008:administration & dosage / Q000235:genetics; D004274:DNA, Recombinant / Q000008:administration & dosage / Q000235:genetics; D015870:Gene Expression; D020868:Gene Silencing; D005821:Genetic Techniques; D005822:Genetic Vectors; D010942:Plant Viruses / Q000235:genetics; D030821:Plants, Genetically Modified / Q000235:genetics*; D010957:Plasmids / Q000008:administration & dosage / Q000235:genetics; D012093:Replicon; D014162:Transfection; D014170:Transformation, Genetic; D014908:Triticum / Q000235:genetics
D004269:DNA, Bacterial; D004274:DNA, Recombinant; C036483:T-DNA