Reconstruction of high-resolution holographic microscopic images.
In in-line holographic microscopy a pinhole illuminates an object and a CCD-detector directly records the hologram in a pixel-pitch-dependent distance. A rapidly calculating exact reconstruction technique using a reorganized hologram with a low number of pixels, the tile superposition technique, is presented. The algorithm is applied on imaging of a 2 microm bead cluster, and it is compared with other reconstruction techniques. The high-contrast image corresponds to an NA of 0.7. A full 4 megapixel reconstruction with a resolution approaching the diffraction limit is possible in less than a minute. The technique is a base for high-resolution wide-field imaging by multispot illumination.
Institute of Photonic Technology, Albert-Einstein-Strasse 9, 07745 Jena, Germany. mario.kanka@ipht-jena.de
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M
Kanka
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R
Riesenberg
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HJ
Kreuzer