Study of the effect of lethal and sublethal pH and a(w) stresses on the inactivation or growth of Listeria monocytogenes and Salmonella Typhimurium.
During food processing, microorganisms are commonly exposed to multiple sublethal or lethal stresses (commonly a(w), pH) sequentially or simultaneously. The objectives of the present study were: (i) to comparatively evaluate the survival of Listeria monocytogenes and Salmonella Typhimurium in lethal acid (pH 4.0 and 4.5 with lactic acid) or osmotic conditions (15 and 20% NaCl), applied singly, sequentially (pH then NaCl or NaCl then pH), or simultaneously at 5 and 10 degrees C; and, (ii) to quantify the effect of osmotic shifts at pH 7.0, 6.0 or 5.0 (adjusted with lactic acid) on the lag phase and growth rate of L. monocytogenes at 10 degrees C. In sequential lethal stress applications, the second stress was applied 2 or 3 days after the first for Salmonella and L. monocytogenes, respectively. Acid tolerance of L. monocytogenes was higher than osmotic tolerance and the opposite was observed for Salmonella. Higher inactivation was observed after exposure to pH 4.0 compared to pH 4.5 as well as after exposure to 20% NaCl compared to 15% NaCl. Exposure to stresses sequentially resulted in faster (P<0.05) reductions than the exposure to single or double stresses applied simultaneously. The pH then NaCl sequence was more detrimental for pathogens than the reverse sequence. Incubation temperature (5 and 10 degrees C) did not show any profound (P<0.05) effect on microbial inactivation. When L. monocytogenes was incubated at a(w) 0.930 or 0.995 at 30 degrees C, then the lag phase increased both in subsequent osmotic downshift and upshift, respectively, at 10 degrees C. Shorter lag phase and higher ability to initiate growth at lower a(w) was observed after pre-adaptation at pH 6.0 or 5.0 compared to neutral pH. The results may contribute to the review of critical limits in low pH (with lactic acid) and water activity products, considering the risk of L. monocytogenes and Salmonella survival. In addition, the present indications may address the points in processing where stricter sanitation procedures should be applied in order to minimize the risk of survivors.
Agricultural University of Athens, Department of Food Science & Technology, Laboratory of Food Hygiene and Quality Control, Iera Odos 75, Athens, 11855, Greece.
Alexandros
A
Tiganitas
Nikoleta
N
Zeaki
Antonia S
AS
Gounadaki
Eleftherios H
EH
Drosinos
Panagiotis N
PN
Skandamis
D000222:Adaptation, Physiological; D015169:Colony Count, Microbial; D003257:Consumer Product Safety; D004305:Dose-Response Relationship, Drug; D005506:Food Contamination / Q000517:prevention & control; D005511:Food Handling / Q000379:methods*; D005516:Food Microbiology; D006863:Hydrogen-Ion Concentration; D008089:Listeria monocytogenes / Q000187:drug effects / Q000254:growth & development* / Q000502:physiology; D008954:Models, Biological; D009994:Osmolar Concentration; D012486:Salmonella typhimurium / Q000187:drug effects / Q000254:growth & development* / Q000502:physiology; D012965:Sodium Chloride / Q000494:pharmacology; D013696:Temperature; D014867:Water / Q000378:metabolism*
D014867:Water; D012965:Sodium Chloride