Analysis of bla(SHV) codon 238 and 240 allele mixtures using Sybr green high-resolution melting analysis.
Klebsiella pneumoniae isolates frequently contain complex mixtures of bla(SHV) alleles. A high-resolution melting-based method for interrogating the extended-spectrum activity conferring codon 238 and 240 polymorphisms was developed. This detects minority extended-spectrum beta-lactamase-encoding alleles, allows estimation of allele ratios, and discriminates between single and double mutants.
Menzies School of Health Research, Institute of Advanced Studies, Charles Darwin University, Northern Territory 0810, Australia.
Patiyan
P
Andersson
Tegan
T
Harris
Steven Y C
SY
Tong
Philip M
PM
Giffard
D000483:Alleles; D052160:Benzothiazoles; D003062:Codon; D003959:Diamines; D007711:Klebsiella pneumoniae / Q000235:genetics; D009930:Organic Chemicals; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide; D011804:Quinolines; D001618:beta-Lactamases / Q000235:genetics*
D052160:Benzothiazoles; D003062:Codon; D003959:Diamines; D009930:Organic Chemicals; D011804:Quinolines; C098022:SYBR Green I; C054882:beta-lactamase PIT-2; C107197:beta-lactamase SHV-11; C107198:beta-lactamase SHV-12; C062760:beta-lactamase SHV-2; C117193:beta-lactamase SHV-5; D001618:beta-Lactamases