Strain-dependent inflammatory responsiveness of rat microglial cells.
The aim of this study is to test whether inflammatory responsiveness of rat microglial cells is strain-specific in primary microglia derived from neonatal LEW/N and F344/N rats. In contrast to F344/N microglia, LEW/N microglia constitutively and upon lipopolysaccharide challenge expressed higher levels of mRNA for the majority of inflammatory mediators studied. In addition, LEW/N microglia exhibited enhanced secretion of tumor necrosis factor-alpha and CCL2, as well as elevated nitric oxide production. On the contrary, activated LEW/N microglia transcribed and secreted less interleukin-10. Hence, compared to F344/N microglia, LEW/N microglia might be more reactive to lipopolysaccharide and incompetent to suppress inflammation.
National Health Research Institutes, Zhunan Town, Miaoli County, Taiwan. rwei@nhri.org.tw
Rongtai
R
Wei
Chiao-Mei
CM
Lin
D000818:Animals; D002478:Cells, Cultured; D018932:Chemokine CCL2 / Q000096:biosynthesis / Q000276:immunology; D020869:Gene Expression Profiling; D007249:Inflammation / Q000276:immunology*; D018836:Inflammation Mediators / Q000276:immunology* / Q000378:metabolism; D016753:Interleukin-10 / Q000096:biosynthesis / Q000276:immunology; D008070:Lipopolysaccharides / Q000276:immunology; D017628:Microglia / Q000276:immunology* / Q000378:metabolism; D009569:Nitric Oxide / Q000096:biosynthesis / Q000276:immunology; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D011916:Rats, Inbred F344; D011917:Rats, Inbred Lew; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis / Q000276:immunology
D018932:Chemokine CCL2; D018836:Inflammation Mediators; D008070:Lipopolysaccharides; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10; D009569:Nitric Oxide