Fully automated, sequential tilt-series acquisition with Leginon.
Electron tomography has become a uniquely powerful tool for investigating the structures of individual cells, viruses, and macromolecules. Data collection is, however, time consuming and requires expensive instruments. To optimize productivity, we have incorporated one of the existing tilt-series acquisition programs, UCSF Tomo, into the well-developed automatic electron microscopy data collection package Leginon to enable fully automatic, sequential tilt-series acquisition. Here we describe how UCSF Tomo was integrated into Leginon, what users must do to set up a data collection session, how the automatic collection proceeds, how archived data about the process can be accessed and used, and how the software has been tested.
Suloway
Christian
C
Division of Biology, California Institute of Technology, 1200 E. California Blvd., Pasadena, CA 91125, USA.
Shi
Jian
J
Cheng
Anchi
A
Pulokas
James
J
Carragher
Bridget
B
Potter
Clinton S
CS
Zheng
Shawn Q
SQ
Agard
David A
DA
Jensen
Grant J
GJ
D020285:Cryoelectron Microscopy; D055032:Electron Microscope Tomography / Q000379:methods*; D046529:Microscopy, Electron, Transmission; D012984:Software