Detection of stx1, stx2, and eae genes of enterohemorrhagic Escherichia coli using SYBR Green in a real-time polymerase chain reaction.
We report a multiplex real-time polymerase chain reaction method for detecting enterohemorrhagic Escherichia coli (EHEC) from strains or stool specimens. This assay detected the virulence genes stx1, stx2, and eae, without the use of probes. The method, which was validated on a collection of 143 EHEC strains, is simple, rapid, cost-effective, and sensitive.
CHU Clermont-Ferrand, Centre de Biologie, Laboratoire de Bactériologie Clinique, F-63003 Clermont-Ferrand, France.
Laurent
L
Chassagne
Nathalie
N
Pradel
Frédéric
F
Robin
Valérie
V
Livrelli
Richard
R
Bonnet
Julien
J
Delmas
D018829:Adhesins, Bacterial / Q000235:genetics*; D052160:Benzothiazoles; D002648:Child; D003959:Diamines; D054324:Enterohemorrhagic Escherichia coli / Q000235:genetics*; D004927:Escherichia coli Infections / Q000382:microbiology*; D029968:Escherichia coli Proteins / Q000235:genetics*; D005243:Feces / Q000382:microbiology; D006801:Humans; D009930:Organic Chemicals / Q000378:metabolism*; D016133:Polymerase Chain Reaction / Q000379:methods*; D011804:Quinolines; D022622:Shiga Toxin 1 / Q000235:genetics*; D022641:Shiga Toxin 2 / Q000235:genetics*; D013194:Staining and Labeling; D037521:Virulence Factors / Q000235:genetics
D018829:Adhesins, Bacterial; D052160:Benzothiazoles; D003959:Diamines; D029968:Escherichia coli Proteins; D009930:Organic Chemicals; D011804:Quinolines; D022622:Shiga Toxin 1; D022641:Shiga Toxin 2; D037521:Virulence Factors; C073819:eaeA protein, E coli; C098022:SYBR Green I