The specific effect of 2-methoxyestradiol on lymphatic vascular endothelial cells.
Lymphatic metastasis of tumors is one of the most important prognostic factors and provides valuable information for decisions on appropriate surgical protocols. Recent studies have demonstrated that lymphangiogenesis of lymphatic vascular endothelial cells into tumors is a key event in lymphatic metastasis. Therefore, control of lymphangiogenesis is a promising strategy for treatment or prevention of tumor metastasis and lymphatic disorders. However, mechanisms of lymphangiogenesis or its specific inhibition are not well-understood. In this study we examined effects of various types of signaling inhibitors on tube formation in human lymphatic microvascular endothelial cells (LECs) and blood microvascular endothelial cells (BECs) in vitro. We found that tube formation of LECs was specifically inhibited by 2-methoxyestradiol (2ME). This observation is of potential benefit in understanding the molecular mechanism of lymphangiogenesis. Furthermore, 2ME could therefore offer specific protection against lymphatic metastasis and lymphangiogenesis-related diseases.
Laboratory of Pharmaceutical Proteomics, National Institute of Biomedical Innovation (NIBIO), Ibaraki, Osaka, Japan.
S
S
Imai
Y
Y
Yoshida
T
T
Okamura
K
K
Nagano
Y
Y
Abe
T
T
Yoshikawa
H
H
Kamada
S
S
Nakagawa
S
S
Tsunoda
Y
Y
Tsutsumi
D000077584:2-Methoxyestradiol; D001808:Blood Vessels / Q000187:drug effects; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D042783:Endothelial Cells / Q000187:drug effects*; D004729:Endothelium, Lymphatic / Q000166:cytology* / Q000187:drug effects / Q000254:growth & development; D004730:Endothelium, Vascular / Q000166:cytology / Q000187:drug effects; D004958:Estradiol / Q000031:analogs & derivatives* / Q000494:pharmacology; D006801:Humans; D042583:Lymphangiogenesis / Q000187:drug effects*; D018919:Neovascularization, Physiologic / Q000187:drug effects; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction
D012333:RNA, Messenger; D004958:Estradiol; D000077584:2-Methoxyestradiol