Infrared microspectroscopy analysis of ibuprofen release from drug eluting beads in uterine tissue.
Ibuprofen loaded embolization beads (IBU-BB) have been developed to reduce inflammation and pain following uterine artery embolization for the treatment of uterine fibroids. The present work has investigated the elution properties of IBU-BB in situ after embolization with Fourier Transform Infrared Microspectroscopy (FTIRMS). Twelve sheep underwent uterine artery embolization with IBU-BB (485 mM) or control unloaded beads. IBU concentration was determined inside the beads and in the tissue surrounding the beads using FTIRMS of uterine tissue sections sampled 24 h or 1 week after embolization. After 24 h, IBU concentration inside the bead was only 18.6 mM out of the 485 mM initially loaded (p < 0.0001, univariate sign test). The concentration in the tissue around the beads was 8 mM, which is well above the in vitro therapeutic levels (6 microM). After one week the concentration of IBU had decreased to 4.9 mM in the beads (p = 0.0502, Mann Whitney) and no IBU was detected in the surrounding tissue. This work has demonstrated that IBU-BB can provide a sustained release of the anti-inflammatory drug over at least one week. The in vivo elution properties of IBU-BB may be suitable to alleviate pain and inflammation after embolization.
Namur
J
J
MéDyC UMR CNRS 6237, Unité MéDIAN, Université de Reims Champagne Ardennes, 51 Rue Cognaq-Jay, 51096 Reims, France. namurjul@hotmail.fr
Wassef
M
M
Pelage
J P
JP
Lewis
A
A
Manfait
M
M
Laurent
A
A
D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000032:analysis* / Q000493:pharmacokinetics; D002138:Calibration; D003692:Delayed-Action Preparations; D004305:Dose-Response Relationship, Drug; D004621:Embolization, Therapeutic; D005260:Female; D007052:Ibuprofen / Q000032:analysis* / Q000493:pharmacokinetics; D008863:Microspheres; D012680:Sensitivity and Specificity; D034561:Sheep, Domestic; D013055:Spectrophotometry, Infrared; D013056:Spectrophotometry, Ultraviolet; D017550:Spectroscopy, Fourier Transform Infrared; D013997:Time Factors; D014599:Uterus / Q000098:blood supply*
D000894:Anti-Inflammatory Agents, Non-Steroidal; D003692:Delayed-Action Preparations; D007052:Ibuprofen