Platelet GP IIIA polymorphism HPA-1 (PLA1/2) is associated with hypertension as the primary cause for end-stage renal disease in hemodialysis patients from Greece.
Human platelets carry membrane glycoproteins that control platelet aggregation and activation. A number of clinical studies have suggested that certain polymorphisms of genes encoding these proteins increase the risk for cardiovascular disease. The frequency of gene polymorphisms for the four most common platelet glycoproteins (HPA 1, 2, 3 and 5) was examined and correlated with the primary cause of end-stage renal disease (ESRD) in Greek patients on HD. Fifty-five (55) patients on chronic maintenance haemodialysis (HD) (22 female, 33 male), aged from 23- to 87-years-old, (mean age 66 years), being on dialysis for 53 +/- 34 months, were included in the study. HPA-1, -2, -3, and -5 genotyping was performed using polymerase chain reaction (PCR) amplification with sequence-specific primers (PCR-SSP). Calculated relative frequencies of the alleles were as follows: HPA-1a/b 0.81/0.19, HPA-2a/b 0.92/0.08, HPA-3a/b 0.62/0.38 and HPA-5a/b 0.93/0.07. There was a statistically significant association between the HPA-1b allele and hypertension as the primary cause of ESRD (65% of patients with hypertension vs 23% of all other patients carried the HPA-1b allele, p=0.02, Fisher's exact test). The results suggest that Greek carriers of the HPA-1b allele with hypertension may be at increased risk for developing end-stage renal disease.
Chiras
Theodore
T
Renal Unit, Ygeia Hospital, Athens, Greece. hiras@ath.forthnet.gr
Papadakis
Emmanuel D
ED
Katopodi
Aggeliki
A
Chatzianesti
Efrosini
E
Fourtounas
Kostas
K
Papakonstantinou
Stamatina
S
Theodoropoulos
Ilias
I
Dakouras
Anastasios
A
Zerefos
Nikolas
N
Valis
Dimitrios
D
Tzanatos-Exarchou
Helen
H
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D016824:Antigens, Human Platelet / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D006973:Hypertension / Q000150:complications / Q000235:genetics* / Q000473:pathology; D039661:Integrin beta3 / Q000235:genetics*; D007676:Kidney Failure, Chronic / Q000209:etiology / Q000235:genetics* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D011110:Polymorphism, Genetic; D006435:Renal Dialysis; D055815:Young Adult
D016824:Antigens, Human Platelet; C061248:ITGB3 protein, human; D039661:Integrin beta3; C083451:human platelet antigen 1b