Darcian permeability constant as indicator for shear stresses in regular scaffold systems for tissue engineering.
The shear stresses in printed scaffold systems for tissue engineering depend on the flow properties and void volume in the scaffold. In this work, computational fluid dynamics (CFD) is used to simulate flow fields within porous scaffolds used for cell growth. From these models the shear stresses acting on the scaffold fibres are calculated. The results led to the conclusion that the Darcian (k(1)) permeability constant is a good predictor for the shear stresses in scaffold systems for tissue engineering. This permeability constant is easy to calculate from the distance between and thickness of the fibres used in a 3D printed scaffold. As a consequence computational effort and specialists for CFD can be circumvented by using this permeability constant to predict the shear stresses. If the permeability constant is below a critical value, cell growth within the specific scaffold design may cause a significant increase in shear stress. Such a design should therefore be avoided when the shear stress experienced by the cells should remain in the same order of magnitude.
Systems and Control Group, Wageningen University, P.O. Box 17, 6700 AA Wageningen, The Netherlands. petra.vossenberg@wur.nl
Petra
P
Vossenberg
G A
GA
Higuera
G
G
van Straten
C A
CA
van Blitterswijk
A J B
AJ
van Boxtel
D000465:Algorithms; D000818:Animals; D001842:Bone and Bones / Q000473:pathology; D049109:Cell Proliferation; D005109:Extracellular Matrix; D006041:Goats; D059630:Mesenchymal Stem Cells / Q000166:cytology; D015233:Models, Statistical; D010477:Perfusion; D010539:Permeability; D016062:Porosity; D015203:Reproducibility of Results; D033081:Shear Strength; D013314:Stress, Mechanical; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry; D014783:Viscosity