Quantitative analysis of bacterial adhesion to fish tissue.
Adhesion to host tissue represents a first crucial step in most bacterial infections. Both specific adhesion-ligand as well as hydrophobic interactions may be involved. The adhesion of Aeromonas salmonicida subsp. salmonicida, Lactococcus garvieae, and Yersinia ruckeri strains to fish tissue cells was assessed. To determine whether the observed bacterial adhesion to fish tissue cells was caused by non-specific interactions, adhesion to bovine serum albumin (BSA) and polystyrene was also tested. Our results demonstrated that non-specific adhesion such as hydrophobic interactions are only partially involved in the binding process since adhesion to BSA was low, and there was no correlation between adhesion to polystyrene and adhesion to fish tissue cells.
Vendrell
Daniel
D
Laboratory of Fish Pathology, Faculty of Veterinary Sciences, University of Zaragoza, c/. Miguel Servet 177, Zaragoza 50013, Spain.
Balcázar
José Luis
JL
Calvo
Ana Cristina
AC
de Blas
Ignacio
I
Ruiz-Zarzuela
Imanol
I
Gironés
Olivia
O
Múzquiz
José Luis
JL
D000818:Animals; D001422:Bacterial Adhesion; D002469:Cell Separation; D017686:Oncorhynchus mykiss / Q000382:microbiology*; D009928:Organ Specificity