Nanoscale film formation of ferritin and its application to biomemory device.
A redox protein, ferritin is used as a functional constituent of the developed biomemory device. The concept of molecular device mainly depends on the solidification of biomolecules of interest and on the realization of properties of molecule immobilized on a selected substrate. Here, we immobilized the biomolecule, ferritin protein on gold substrate using an organic linker 11-mercaptoundecanoic acid (11-MUA). The immobilization of the protein on the gold substrate was confirmed by surface plasmon spectroscopy, Raman spectroscopy, and atomic force microscopy (AFM). The basic two memory functions, reading and writing of the developed biomemory device, were investigated by open-circuit potential amperometry (OCPA) using the redox property of the biomolecule of interest. The surface topography investigation by scanning tunneling microscopy (STM) shows that the robustness of the ferritin-based biomemory device was validated by the repeated electrochemical performance. These results show the developed biomemory device as a step towards the protein-based nanobiochip.
Kim
Sang-Uk
SU
Interdisciplinary Program of Integrated Biotechnology, Sogang University, #1 Shinsu-dong, Mapo-gu, Seoul 121-742, Republic of Korea.
Lee
Taek
T
Lee
Jin-Ho
JH
Yagati
Ajay Kumar
AK
Min
Junhong
J
Choi
Jeong-Woo
JW
D004560:Electricity; D005293:Ferritins / Q000378:metabolism*; D006046:Gold / Q000378:metabolism; D046208:Microchip Analytical Procedures; D018625:Microscopy, Atomic Force; D011485:Protein Binding; D013059:Spectrum Analysis, Raman; D020349:Surface Plasmon Resonance
D006046:Gold; D005293:Ferritins