Introduction: cell-based assays for high-throughput screening.
Cell-based assays represent approximately half of all high-throughput screens (HTS) currently performed. Here we review the history and status of HTS, and summarize some of the challenges and benefits associated with the use of cell-based assays in HTS, drawing upon themes that will reemerge in subsequent chapters in this book. Approaches for successful experimental design and execution of cell-based HTS are introduced, including strategies for assay development, implementation of primary and secondary screens, and target identification. In doing so, we hope to provide a comprehensive review of the cell-based HTS process and an introduction to the methodologies and techniques described in this book.
An
W Frank
WF
Chemical Biology Platform, Broad Institute of Harvard and MIT, Cambridge, MA, USA.
Tolliday
Nicola J
NJ
D000818:Animals; D001681:Biological Assay / Q000379:methods*; D002477:Cells / Q000187:drug effects*; D020650:Combinatorial Chemistry Techniques / Q000379:methods*; D004353:Drug Evaluation, Preclinical / Q000379:methods*; D006801:Humans; D054852:Small Molecule Libraries
D054852:Small Molecule Libraries