Competitive ELISA using a rabies glycoprotein-transformed cell line to semi-quantify rabies neutralizing-related antibodies in dogs.
Dogs are the principal vectors for rabies virus transmission to humans in many parts of the world. The current "gold standard" World Health Organization- and Office International des Epizooties-recommended tests for measuring anti-rabies virus antibodies in dogs, such as the fluorescent antibody virus neutralization (FAVN) test, requires high containment facilities and several days for results. Here, we describe a new competitive ELISA (c-ELISA) that utilizes a cell line stably expressing the rabies virus glycoprotein as the coating antigen and two horseradish peroxidase-labeled monoclonal rabies-neutralizing antibodies as indicator, which can be carried out in any basic laboratory. We demonstrate that the c-ELISA has high specificity and sensitivity. Application to 4350 dog serum samples showed a parallel relationship with the FAVN at neutralizing antibody levels up to 8.00 IU/ml. Since it can assay 40 or more samples in 1.5h, the c-ELISA is suitable for the surveillance of mass vaccination in dogs where rabies causes public concern.
Laboratory of Epidemiology, Veterinary Institute, Academy of Military Medical Sciences, 1068 Qinglong Road, Changchun 130062, PR China.
Shoufeng
S
Zhang
Ye
Y
Liu
Fei
F
Zhang
Rongliang
R
Hu
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000914:Antibodies, Viral / Q000097:blood*; D000956:Antigens, Viral / Q000276:immunology; D002461:Cell Line, Transformed; D004283:Dog Diseases / Q000276:immunology*; D004285:Dogs; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006023:Glycoproteins / Q000276:immunology*; D009500:Neutralization Tests; D011818:Rabies / Q000276:immunology / Q000662:veterinary*; D011819:Rabies Vaccines / Q000276:immunology; D011820:Rabies virus / Q000276:immunology; D012680:Sensitivity and Specificity; D014759:Viral Envelope Proteins / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D000956:Antigens, Viral; D006023:Glycoproteins; D011819:Rabies Vaccines; D014759:Viral Envelope Proteins