Endothelial cell-based methods for the detection of cyanobacterial anti-inflammatory and wound-healing promoting metabolites.
Acute lung injury is accompanied by an increased endothelial chemokine production and adhesion molecule expression, which may result in an extensive neutrophil infiltration. Moreover, a destruction of the alveolar epithelium and capillary endothelium may result in permeability edema. As such, the search for novel anti-inflammatory substances, able to downregulate these parameters as well as the tissue damage holds therapeutic promise. We therefore describe here the use of human endothelial cell-based in vitro assays for the detection of anti-inflammatory and wound-healing metabolites from cyanobacteria.
Wiesner
Christoph
C
Vascular Biology Center, Medical College of Georgia, Augusta, GA30909, USA. christoph.wiesner@fh-krems.ac.at
Kopecky
Jiri
J
Pflueger
Maren
M
Hundsberger
Harald
H
Entler
Barbara
B
Kleber
Christoph
C
Atzler
Josef
J
Hrouzek
Pavel
P
Stys
Dalibor
D
Lukesova
Alena
A
Schuett
Wolfgang
W
Lucas
Rudolf
R
D055371:Acute Lung Injury / Q000188:drug therapy / Q000503:physiopathology; D000893:Anti-Inflammatory Agents / Q000302:isolation & purification / Q000494:pharmacology*; D002460:Cell Line; D042783:Endothelial Cells / Q000187:drug effects / Q000378:metabolism; D005786:Gene Expression Regulation; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism; D008954:Models, Biological; D020556:Neutrophil Infiltration / Q000187:drug effects; D046937:Nostoc / Q000737:chemistry* / Q000378:metabolism; D011650:Pulmonary Alveoli / Q000187:drug effects / Q000503:physiopathology; D014945:Wound Healing / Q000187:drug effects*
D000893:Anti-Inflammatory Agents; D018799:Intercellular Adhesion Molecule-1