Detection of tetracycline and oxytetracycline residues in pig and calf hair by ultra-high-performance liquid chromatography tandem mass spectrometry.
An ultra-high-performance liquid chromatography tandem mass spectrometry method to detect residues of tetracycline (TC), epi-tetracycline (eTC) and oxytetracycline (OTC) in animal hair was developed. Hair samples were washed with water, extracted with NH(4)OH 0.1M, purified by SPE-C(18) cartridge and analyzed by tandem mass spectrometry (ESI(+), MRM mode) with satisfactory results. For the first time, accumulation of TC, eTC and OTC was confirmed in livestock hairs after a therapeutic treatment with TC and OTC, respectively. Administered drug residues were detectable in hair samples up to 2 months after the last treatment, providing a retrospective evidence of TC and OTC administration. Hair analysis seems to offer a wider window of detection than edible tissues.
Castellari
M
M
IRTA-Monells, Monells Girona, Spain. massimo.castellari@irta.cat
Gratacós-Cubarsí
M
M
García-Regueiro
J A
JA
D000818:Animals; D000900:Anti-Bacterial Agents / Q000008:administration & dosage / Q000032:analysis*; D001424:Bacterial Infections / Q000188:drug therapy / Q000662:veterinary; D002417:Cattle; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004350:Drug Residues / Q000032:analysis*; D006197:Hair / Q000737:chemistry*; D009132:Muscles / Q000737:chemistry; D010118:Oxytetracycline / Q000008:administration & dosage / Q000032:analysis*; D013552:Swine; D053719:Tandem Mass Spectrometry / Q000379:methods*; D013752:Tetracycline / Q000008:administration & dosage / Q000032:analysis*
D000900:Anti-Bacterial Agents; D013752:Tetracycline; D010118:Oxytetracycline