Determination of 2-ethylhexyl 4-(dimethylamino) benzoate using membrane-assisted liquid-liquid extraction and gas chromatography-mass spectrometric detection.
A flow-cell for micro-porous membrane liquid-liquid extraction with a sheet membrane was used to extract 2-ethylhexyl 4-(dimethylamino) benzoate (EDB) from urine of solar-cream users and spiked wine samples. The cell enabled the target analyte to be extracted from 7.9 mL of donor solution into 200 microL of acceptor solution (decane). After extraction, the acceptor solution was transferred to a micro-vial for GC-MS analysis without derivation. In this work, variables affecting the enrichment factor were also studied, such as organic solvent, extraction time, recirculation flow of the donor solution through the donor chamber, presence of potassium chloride and ethanol in the donor solution and pH. The method has been evaluated in terms of linearity, sensitivity, precision, limits of detection and quantification and extraction efficiency. Limits of quantification were 1 and 3 microg L(-1) EDB for urine and wine, respectively. Quantitative analysis has been carried out by applying the method of standard additions. Within- and between-day relative standard deviations were lower than 12% and 20%, respectively. EDB was found in the urine of users of cream containing EDB in the concentration interval 1.2-7.2 microg L(-1). Therefore, this provides evidence of EDB dermal absorption and subsequent excretion through the urinary tract. EDB was not found in the analysed wine samples.
Department of Chemistry, University of Balearic Islands, 07122, Palma de Mallorca, Spain. joan.march@uib.es
J G
JG
March
C
C
Genestar
B M
BM
Simonet
D010129:4-Aminobenzoic Acid / Q000652:urine; D005591:Chemical Fractionation / Q000295:instrumentation / Q000379:methods*; D000431:Ethanol / Q000737:chemistry; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008567:Membranes, Artificial; D016062:Porosity; D011189:Potassium Chloride / Q000737:chemistry; D013499:Surface Properties; D013997:Time Factors; D014920:Wine / Q000032:analysis*; D062366:para-Aminobenzoates
D008567:Membranes, Artificial; D062366:para-Aminobenzoates; D000431:Ethanol; D011189:Potassium Chloride; D010129:4-Aminobenzoic Acid; C041981:padimate-O