Chemiluminescence determination of tetracyclines using Fenton system in the presence europium(III) ions.
A new simple chemiluminescent method for the determination of chlortetracycline (Chlor-TC), oxytetracycline (Oxy-TC) and doxycycline (Doxy-TC) is described. This method is based on the europium(III) emission as a result of the energy transfer process from the excited product of the tetracyclines oxidation to the uncomplexed Eu(III). Under the optimum conditions, calibration graphs were obtained for 4x10(-7) to 2x10(-5) mol L(-1) of Chlor-TC; 2x10(-7) to 2x10(-5) mol L(-1) of Oxy-TC and 1x10(-7) to 3x10(-5) mol L(-1) of Doxy-TC. The method was successfully applied to the determination of these drugs in pharmaceutical and veterinary formulation and honey.
Kaczmarek
Malgorzata
M
Department of Rare Earths, Faculty of Chemistry, Adam Mickiewicz University, Grunwaldzka 6, 60 - 780 Poznań, Poland.
Lis
Stefan
S
D005063:Europium / Q000737:chemistry*; D006722:Honey / Q000032:analysis; D006861:Hydrogen Peroxide / Q000737:chemistry*; D007501:Iron / Q000737:chemistry*; D007700:Kinetics; D049449:Luminescence; D008163:Luminescent Measurements / Q000379:methods*; D004364:Pharmaceutical Preparations / Q000737:chemistry; D013754:Tetracyclines / Q000032:analysis* / Q000737:chemistry; D014867:Water / Q000737:chemistry
C045076:Fenton's reagent; D004364:Pharmaceutical Preparations; D013754:Tetracyclines; D014867:Water; D005063:Europium; D006861:Hydrogen Peroxide; D007501:Iron