Fabrication of a novel layer-by-layer film based glucose biosensor with compact arrangement of multi-components and glucose oxidase.
Layer-by-layer (LbL) film based glucose biosensor was fabricated with alternative layers of a nanocomposite (comprising of multiwalled carbon nanotubes (MWNTs), Au nanoparticles (Au NPs) and thiol functionalized polyaniline (PANI(SH)) and glucose oxidase (GOx). The successful formation of multilayers was confirmed by UV-visible spectroscopy. The components in the nanocomposite provide adequate electron transfer path between GOx and the electrode. A high value for the rate constant of electron transfer process (27.84 s(-1)) was observed at [GOx/Au-(SH)PANI-g-MWNT](n)/ITO electrode. The [GOx/Au-(SH)PANI-g-MWNT](n) biosensor exhibited high sensitivity (3.97 microA/mM) for the detection of glucose over a concentration range of 1-9 mM with a low detection limit of 0.06 microM.
Komathi
Shanmugasundaram
S
Department of Chemistry Graduate School, Kyungpook National University, Daegu 702-701, South Korea.
Gopalan
Anantha Iyengar
AI
Lee
Kwang-Pill
KP
D000814:Aniline Compounds; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D004800:Enzymes, Immobilized; D004867:Equipment Design; D005947:Glucose / Q000032:analysis*; D005949:Glucose Oxidase; D006046:Gold; D053768:Metal Nanoparticles; D053761:Nanocomposites; D037742:Nanotubes, Carbon; D055672:Static Electricity
D000814:Aniline Compounds; D004800:Enzymes, Immobilized; D037742:Nanotubes, Carbon; C416807:polyaniline; D006046:Gold; D005949:Glucose Oxidase; D005947:Glucose