Facile single step fabrication of microchannels with varying size.
In this report, we present a non-lithographic embedded template method for rapid and cost-effective fabrication of a monolithic microfluidic device with channels of various sizes. The procedure presented here enables the preparation of microchannels with varying dimensions in a single device without using any sophisticated micromachining instrumentation. In addition, this non-lithographic technique has also been used to fabricate a multilayer-multilevel biopolymer microdevice in a single step. To demonstrate the versatility of the presented method, we have fabricated microfluidic devices with four different materials under different curing/cross linking conditions. We have also demonstrated the application of the fabricated device to generate structured copper alginate microbeads, in vitro protein synthesis in three phase flow, and alternate plugs with liquid spacers.
Asthana
Amit
A
Department of Fine Chemical Engineering and Applied Chemistry, Chungnam National University, Daejeon 305-764, Korea.
Kim
Kyeong-Ohn
KO
Perumal
Jayakumar
J
Kim
Dong-Myung
DM
Kim
Dong-Pyo
DP
D000464:Alginates / Q000138:chemical synthesis / Q000737:chemistry; D004867:Equipment Design; D029968:Escherichia coli Proteins / Q000138:chemical synthesis / Q000737:chemistry; D020723:Glucuronic Acid / Q000138:chemical synthesis / Q000737:chemistry; D006603:Hexuronic Acids / Q000138:chemical synthesis / Q000737:chemistry; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation / Q000379:methods*; D008855:Microscopy, Electron, Scanning; D008863:Microspheres; D010316:Particle Size; D016133:Polymerase Chain Reaction; D013499:Surface Properties
D000464:Alginates; D029968:Escherichia coli Proteins; D006603:Hexuronic Acids; D020723:Glucuronic Acid