Reversible immobilization of Candida rugosa lipase on fibrous polymer grafted and sulfonated p(HEMA/EGDMA) beads.
Poly(2-hydroxyethyl methacrylate/ethylenglycol dimethacrylate) beads were grafted with poly(glycidylmethacrylate) via surface initiated atom transfer radical polymerization. Epoxy groups of the grafted polymer were modified in to sulfone groups. Sulfonated beads were characterized by swelling studies, FT-IR, SEM and elemental analysis, and were used for reversible immobilization of lipase. Under given experimental conditions, the beads had an adsorption capacity of 44.7 mg protein/g beads. The adsorbed lipase on beads retained up to 67.4% of its initial activity. The immobilized lipase exhibited improved thermal and storage stabilities over those of the free enzyme. The immobilized lipase could desorb 1.0 M NaCl solution at pH 8.0, and the sulfonated beads can be repeatedly charged with fresh enzyme after inactivation upon use.
Biochemical Processing and Biomaterial Research Laboratory, Faculty of Arts and Sciences, Gazi University, Teknik Okullar, Ankara, Turkey. yakuparica@tnn.net
M
M
Yakup Arica
Hande
H
Soydogan
Gulay
G
Bayramoglu
D000327:Adsorption; D001709:Biotechnology / Q000379:methods*; D002175:Candida / Q000201:enzymology*; D004800:Enzymes, Immobilized / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008049:Lipase / Q000737:chemistry*; D008689:Methacrylates / Q000737:chemistry*; D008855:Microscopy, Electron, Scanning / Q000379:methods; D011108:Polymers / Q000737:chemistry; D011208:Powders; D017550:Spectroscopy, Fourier Transform Infrared; D013447:Sulfites / Q000737:chemistry; D013450:Sulfones / Q000737:chemistry
D004800:Enzymes, Immobilized; D008689:Methacrylates; D011108:Polymers; D011208:Powders; D013447:Sulfites; D013450:Sulfones; C005044:hydroxyethyl methacrylate; C004919:ethylene dimethacrylate; D008049:Lipase; C025026:sodium sulfite