Suspension-adapted Chinese hamster ovary-derived cells expressing green fluorescent protein as a screening tool for biomaterials.
Synthetic biomaterials play an important role in regenerative medicine. To be effective they must support cell attachment and proliferation in addition to being non-toxic and non-immunogenic. We used a suspension-adapted Chinese hamster ovary-derived cell line expressing green fluorescent protein (GFP) to assess cell attachment and growth on synthetic biomaterials by direct measurement of GFP-specific fluorescence. To simplify operations, all cell cultivation steps were performed in orbitally-shaken, disposable containers. Comparative studies between this GFP assay and previously established cell quantification assays demonstrated that this novel approach is suitable for rapid screening of a large number of samples. Furthermore the utility of our assay system was confirmed by evaluation of cell growth on three polyvinylidene fluoride polymer scaffolds that differed in pore diameter and drawing conditions. The data presented here prove the general utility of GFP-expressing cell lines and orbital shaking technology for the screening of biomaterials for tissue engineering applications.
Laboratory of Cellular Biotechnology, Ecole Polytechnique Fédérale de Lausanne, School of Life Sciences, 1015, Lausanne, Switzerland.
E-M
EM
Engelhardt
S
S
Houis
T
T
Gries
J
J
Hilborn
M
M
Adam
F M
FM
Wurm
D000818:Animals; D001672:Biocompatible Materials / Q000633:toxicity*; D016466:CHO Cells / Q000187:drug effects*; D002448:Cell Adhesion / Q000187:drug effects; D018929:Cell Culture Techniques; D049109:Cell Proliferation / Q000187:drug effects; D006224:Cricetinae; D003412:Cricetulus; D017930:Genes, Reporter; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D008403:Mass Screening / Q000379:methods*
D001672:Biocompatible Materials; D049452:Green Fluorescent Proteins