Temperature and pH-stability of commercial stationary phases.
In this paper, the temperature and pH stability of silica-based RP stationary phases were investigated. Furthermore, nonsiliceous phases like a polymeric column based on polystyrene divinylbenzene and a polybutadiene coated zirconium dioxide column were also included. The columns were heated up to 150 degrees C at dynamic conditions, which means that the eluent consisting of water and methanol (90:10, v/v) was continuously purged through the packed bed. After every 5 h, the columns were cooled down to room temperature and the efficiency was measured by injecting a test sample based on the Neue test. It could be shown that some stationary phases exhibited a very good temperature stability at the test conditions specified above.
Teutenberg
Thorsten
T
Institut fuer Energie- und Umwelttechnik e. V., Duisburg, Germany. teutenberg@iuta.de
Hollebekkers
Koen
K
Wiese
Steffen
S
Boergers
Andrea
A