Gastrulation defective protease interacts with anionic components of the Drosophila ovary extracellular matrix.
The Drosophila proteases Gastrulation Defective and Snake function in embryonic polarity establishment and bind heparin, a surrogate for anionic species present in the extracellular matrix. Here we demonstrate binding of GD, but not Snake, to anionic species that appear to be tightly associated with a highly purified eggshell matrix.
Department of Cellular Biology and Anatomy, Medical College of Georgia, 1120 15th Street, CB1101, Augusta GA 30912, USA. elemosy@mcg.edu
Sangeetha
S
Sukumari-Ramesh
Ellen K
EK
LeMosy
D000818:Animals; D002478:Cells, Cultured; D004330:Drosophila / Q000235:genetics; D029721:Drosophila Proteins / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D016326:Extracellular Matrix Proteins / Q000378:metabolism*; D005260:Female; D010053:Ovary / Q000378:metabolism; D012697:Serine Endopeptidases / Q000378:metabolism*
D029721:Drosophila Proteins; D016326:Extracellular Matrix Proteins; D012697:Serine Endopeptidases; C440488:gd protein, Drosophila; C081508:snk protein, Drosophila