Changes in Borna disease virus genome with adaptation to host.
The CRNP5 variant of Borna disease virus (BDV) has stronger pathogenesis than the CRP3 variant in which only 4 nucleotides in the whole genome are different. The CRP3 is produced by 3 passages in rat brains of BDV, whereas the CRNP5 is produced by 5 passages in mouse brains after 2 passages in rat brains of the BDV. Thymidylic acids at nt 3608 and 3673 were replaced by cytidylic acids during 3 passages in mice. Three passages in mice caused replacement of adenylic acid at nt 7936 by guanylic acid. No replacement at nt 8742 occurred during passages in mice.
Laboratory of Veterinary Immunology, Azabu University School of Veterinary Medicine, Fuchinobe, Sagamihara, Japan.
Satoshi
S
Okayama
Nanako
N
Miura
Masaru
M
Murakami
Masayuki
M
Funaba
Yoshii
Y
Nishino
D000220:Adaptation, Biological; D000818:Animals; D000831:Animals, Newborn; D001891:Borna disease virus / Q000235:genetics* / Q000254:growth & development*; D001921:Brain / Q000821:virology; D016679:Genome, Viral; D051379:Mice; D017354:Point Mutation; D051381:Rats; D012692:Serial Passage