Olive oil phenolic compounds inhibit homocysteine-induced endothelial cell adhesion regardless of their different antioxidant activity.
In this study, we examine the effect of extra virgin olive oil phenolic compounds on homocysteine-induced endothelial dysfunction and whether the protective effects are related to their different scavenging activities. Structurally related compounds have been assayed for their ability to reduce homocysteine-induced monocyte adhesion as well as the cell surface expression of intercellular adhesion molecule-1 (ICAM-1) in EA.hy.926 cells. As well-known, among the selected phenolic compounds, hydroxytyrosol, homovanillyl alcohol, and the hydroxycinnamic acid derivatives caffeic and ferulic acid display high scavenging activities, while tyrosol and p-coumaric acid are poorly active. All of the tested compounds, approaching potential in vivo concentrations, significantly reduce homocysteine-induced cell adhesion and ICAM-1 expression. Interestingly, we report the first evidence that monophenols tyrosol and p-coumaric acid are selectively protective only in homocysteine-activated cells, while they are ineffective in reducing ICAM-1 expression induced by TNFalpha. Finally, we report the synergistic effect of o-diphenolic and monophenolic compounds.
Manna
Caterina
C
Dipartimento di Biochimica e Biofisica F. Cedrangolo, Seconda Università di Napoli, Via De Crecchio no. 7, 80138 Naples, Italy. caterina.manna@unina2.it
Napoli
Daniela
D
Cacciapuoti
Giovanna
G
Porcelli
Marina
M
Zappia
Vincenzo
V
D000975:Antioxidants / Q000494:pharmacology*; D002448:Cell Adhesion / Q000187:drug effects*; D003373:Coumaric Acids / Q000494:pharmacology; D042783:Endothelial Cells / Q000737:chemistry / Q000187:drug effects / Q000502:physiology*; D006710:Homocysteine / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000032:analysis; D000069463:Olive Oil; D010636:Phenols / Q000494:pharmacology*; D010626:Phenylethyl Alcohol / Q000031:analogs & derivatives / Q000494:pharmacology; D010938:Plant Oils / Q000737:chemistry*; D011422:Propionates; D020298:U937 Cells
D000975:Antioxidants; D003373:Coumaric Acids; D000069463:Olive Oil; D010636:Phenols; D010938:Plant Oils; D011422:Propionates; D006710:Homocysteine; D018799:Intercellular Adhesion Molecule-1; C011867:4-hydroxyphenylethanol; C495469:p-coumaric acid; D010626:Phenylethyl Alcohol