Cyto- and hemocompatibility of a biodegradable 3D-scaffold material designed for medical applications.
In this study, the polyester urethane Degrapol (DP) was explored for medical applications. Electrospun DP-fiber fleeces were characterized with regard to fiber morphology, swelling, and interconnectivity of interfiber spaces. Moreover, DP was assayed for cell proliferation and hemocompatibility being a prerequisite to any further in vivo application. It was shown that DP-fiber fleeces produced at different humidity while spinning affects interconnectivity of interfiber spaces, such that the higher the humidity the looser the resulting fiber fleeces. When the spinning target was cooled with dry ice, the resulting DP-fibers remained less fused to each other. However, permeability for fluorescent beads was not significantly increased. Fibroblast adhesion and proliferation occurred in a comparable manner on native as well as on fibronectin or collagen I adsorbed DP-fiber fleeces. On DP-surfaces fibroblasts proliferated equally well as compared with glass or PLGA surfaces or DP-surfaces adsorbed with fibronectin or collagen I. In contrast, human umbilical vein endothelial cells proliferated only after adsorption of DP-surfaces with fibronectin or collagen I, indicating that different cell types respond differently to DP-surfaces. Furthermore, hemocompatibility of DP-surfaces was found to be similar or better to PLGA or stainless steel, both medically used materials. These experiments indicate that DP-fiber fleeces or surfaces might be useful for tissue engineering.
Department of Materials, Cells and Biomaterials, ETH Zurich, Zurich, Switzerland.
Vincent
V
Milleret
Marc
M
Simonet
Anne Greet
AG
Bittermann
Peter
P
Neuenschwander
Heike
H
Hall
D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry / Q000378:metabolism; D018929:Cell Culture Techniques / Q000379:methods; D049109:Cell Proliferation; D003167:Complement Activation; D006801:Humans; D008422:Materials Testing; D051379:Mice; D041681:NIH 3T3 Cells / Q000166:cytology / Q000378:metabolism; D011091:Polyesters / Q000737:chemistry* / Q000378:metabolism; D011140:Polyurethanes / Q000737:chemistry* / Q000378:metabolism; D013499:Surface Properties; D023822:Tissue Engineering / Q000295:instrumentation / Q000379:methods*; D054457:Tissue Scaffolds
D001672:Biocompatible Materials; C104154:DegraPol; D011091:Polyesters; D011140:Polyurethanes