Hydrangeic acid from the processed leaves of Hydrangea macrophylla var. thunbergii as a new type of anti-diabetic compound.
Hydrangeic acid (3-100 microM), a stilbene constituent of the processed leaves of Hydrangea macrophylla var. thunbergii (Hydrangeae Dulcis Folium), promoted adipogenesis of 3T3-L1 cells. Hydrangeic acid significantly increased the amount of adiponectin released into the medium, the uptake of 2-deoxyglucose into the cells, and the translocation of glucose transporter 4 (GLUT4). Hydrangeic acid also increased mRNA levels of adiponectin, peroxisome proliferator-activated receptor gamma2 (PPARgamma2), GLUT4, and fatty acid-binding protein (aP2) while it decreased the expression of tumor necrosis factor alpha (TNF-alpha) mRNA. However, it did not activate PPARgamma directly different from troglitazone in a nuclear receptor cofactor assay system. Furthermore, hydrangeic acid significantly lowered blood glucose, triglyceride, and free fatty acid levels after its administration for 2 weeks at a dose of 200 mg/kg/day (p.o.) in KK-A(y) mice.
Zhang
Hailong
H
Kyoto Pharmaceutical University, Misasagi, Kyoto, Japan.
Matsuda
Hisashi
H
Yamashita
Chihiro
C
Nakamura
Seikou
S
Yoshikawa
Masayuki
M
D041721:3T3-L1 Cells; D050156:Adipogenesis / Q000187:drug effects; D052242:Adiponectin / Q000378:metabolism; D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D003847:Deoxyglucose / Q000378:metabolism; D005786:Gene Expression Regulation / Q000187:drug effects; D051275:Glucose Transporter Type 4 / Q000378:metabolism; D031966:Hydrangea / Q000737:chemistry*; D007004:Hypoglycemic Agents / Q000494:pharmacology*; D008297:Male; D051379:Mice; D009569:Nitric Oxide / Q000096:biosynthesis; D047495:PPAR gamma / Q000819:agonists; D018515:Plant Leaves / Q000737:chemistry; D012459:Salicylates / Q000494:pharmacology*; D013267:Stilbenes / Q000737:chemistry / Q000494:pharmacology*; D014280:Triglycerides / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
D052242:Adiponectin; D051275:Glucose Transporter Type 4; D007004:Hypoglycemic Agents; D047495:PPAR gamma; D012459:Salicylates; D013267:Stilbenes; D014280:Triglycerides; D014409:Tumor Necrosis Factor-alpha; C543195:hydrangeic acid; D009569:Nitric Oxide; D003847:Deoxyglucose