Formation and characterization of acyl carrier protein-linked polyketide synthase extender units.
Polyketide natural products are assembled by the condensation of an initiating precursor, or starter unit, with a series of additional precursors referred to as extender units. While there are a number of polyketide synthase starter units, there are currently only seven known polyketide synthase extender units. Polyketide synthase extender units thioesterified to coenzyme A have been known for some time; however, polyketide synthase extender units thioesterified to acyl carrier proteins (ACPs) have been identified only recently. Two of them, (2R)-hydroxymalonyl-ACP and (2S)-aminomalonyl-ACP, are found in the biosynthetic pathway of the antibiotic zwittermicin A in Bacillus cereus UW85. The focus of this chapter is the in vitro formation of (2R)-hydroxymalonyl-ACP and (2S)-aminomalonyl-ACP and the characterization of these extender units using high performance liquid chromatography and matrix-assisted laser desorption ionization time-of-flight mass spectrometry.
Department of Bacteriology, University of Wisconsin-Madison, Madison, Wisconsin, USA.
Yolande A
YA
Chan
Michael G
MG
Thomas
D000213:Acyl Carrier Protein / Q000737:chemistry / Q000378:metabolism*; D001409:Bacillus cereus / Q000235:genetics / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D015394:Molecular Structure; D010455:Peptides / Q000378:metabolism; D048630:Polyketide Synthases / Q000737:chemistry / Q000378:metabolism*; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D000213:Acyl Carrier Protein; D010455:Peptides; D011994:Recombinant Proteins; C088290:zwittermicin A; D048630:Polyketide Synthases