Validation of a high-performance liquid chromatography method for the determination of (-)-alpha-bisabolol from particulate systems.
A reversed-phase high performance liquid chromatography method has been developed and validated for determination and quantitation of the natural sesquiterpene (-)-alpha-bisabolol. Furthermore the application of the method was done by characterization of chitosan milispheres and liposomes entrapping Zanthoxylum tingoassuiba essential oil, which contains appreciable amount of (-)-alpha-bisabolol. A reversed-phase C(18) column and gradient elution was used with the mobile phase composed of (A) acetonitrile-water-phosphoric acid (19:80:1) and (B) acetonitrile. The eluent was pumped at a flow rate of 0.8 mL/min with UV detection at 200 nm. In the range 0.02-0.64 mg/mL the assay showed good linearity (R(2 )= 0.9999) and specificity for successful identification and quantitation of (-)-alpha-bisabolol in the essential oil without interfering peaks. The method also showed good reproducibility, demonstrating inter-day and intra-day precision based on relative standard deviation values (up to 3.03%), accuracy (mean recovery of 100.69% +/- 1.05%) and low values of detection and quantitation limits (0.0005 and 0.0016 mg/mL, respectively). The method was also robust for showing a recovery of 98.81% under a change of solvent in standard solutions. The suitability of the method was demonstrated by the successful determination of association efficiency of the (-)-alpha-bisabolol in chitosan milispheres and liposomes.
Medical Subject Research Laboratory, Faculty of Pharmacy, Federal University of Bahia, Brazil.
André
A
São Pedro
Cássia
C
Detoni
Domingos
D
Ferreira
Elaine
E
Cabral-Albuquerque
Bruno
B
Sarmento
D048271:Chitosan / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D016014:Linear Models; D008081:Liposomes / Q000737:chemistry; D000081222:Monocyclic Sesquiterpenes; D009822:Oils, Volatile / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012717:Sesquiterpenes / Q000032:analysis*; D032106:Zanthoxylum / Q000737:chemistry
D008081:Liposomes; D000081222:Monocyclic Sesquiterpenes; D009822:Oils, Volatile; D010936:Plant Extracts; D012717:Sesquiterpenes; C445367:bisabolol acetate; D048271:Chitosan