[The application and significance in prenatal diagnosis using G-banding, fluorescence in situ hybridization and comparative genomic hybridization].
OBJECTIVE
To investigate the procedure and the value of G-banding, fluorescence in sit hybridization (FISH) and comparative genomic hybridization (CGH) techniques in prenatal diagnosis.


METHODS
Karyotype analyses with three diagnostic procedures, G-banding, G-banding and FISH, G-banding, FISH and CGH, were performed in the amniotic fluid samples taken from 102 fetuses at gestational ages 16-24 weeks. And the significance was valued in prenatal diagnosis.


RESULTS
In the first procedure of karyotype analysis, 98 cases were diagnosed, 2 cases were not conformed while 2 cases were failed in all 102 cases. In the second procedure, 2 cases were determined, 1 case was not conformed and 1 case was still failed. In the third step, 2 cases were diagnosed. The diagnostic rate of the karyotype reached to 100% (102/102 cases) using all the three procedures. In total, seven cases with chromosomal abnormality were diagnosed. Four cases, 1 case and 2 cases were identified in the first step (4/7, 57.1%), the second (1/7, 14.3%) and the third (2/7, 28.5%), respectively.


CONCLUSIONS
It can help improve the diagnostic rate of chromosomal aberrations and standardize diagnostic procedure to perform the three detecting steps in prenatal diagnosis.
Zhang
Wei-she
WS
Central South University, Changsha, Hunan, 410008 People's Republic of China.
Chen
Qi-neng
QN
Wu
Xin-hua
XH
Liang
Qing-hua
QH
D002869:Chromosome Aberrations / Q000706:statistics & numerical data*; D002871:Chromosome Banding / Q000379:methods; D025063:Chromosome Disorders / Q000175:diagnosis* / Q000235:genetics; D002887:Chromosomes, Human, Pair 18; D055028:Comparative Genomic Hybridization / Q000379:methods; D005260:Female; D005333:Fetus; D005865:Gestational Age; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008607:Intellectual Disability / Q000235:genetics*; D007621:Karyotyping / Q000379:methods; D008297:Male; D009693:Nucleic Acid Hybridization / Q000379:methods; D011247:Pregnancy; D011296:Prenatal Diagnosis / Q000706:statistics & numerical data*; D012307:Risk Factors; D016216:Ultrasonography, Prenatal / Q000379:methods