Antibacterial activity and chemical composition of essential oil of Pamburus missionis.
OBJECTIVE
The aerial parts of Pamburus missionis (Wight) Swingle (Rutaceae) are traditionally used in the treatment of swelling, chronic rheumatism, paralysis, and puerperal diseases.The aim of this work was to investigate the chemical composition and antibacterial activity of essential oil of Pamburus missionis against different bacterial strains.


METHODS
The essential oil was obtained from fresh leaves by hydro distillation method and its chemical composition was analyzed by GC and GC-MS. The antibacterial activity was determined by disc diffusion and micro broth dilution assay.


RESULTS
GC-MS analysis of the essential oil revealed the presence of 1-tridecanol (38.4%), n-hexadecanoic acid (16.1%), oxygenated monoterpenes (14.4%), monoterpene hydrocarbons (3.1%) and eugenol (1.9%) as the major components. The oil exhibited antibacterial activity at 100 and 450 microg against the test organisms with inhibition zones of 7-25 mm and minimal inhibitory concentrations values in the range of 10-100 and >100 mg/ml.


CONCLUSIONS
The present study reveals the antibacterial potency of essential oil of Pamburus missionis. The use of Pamburus missionis in the treatment of various ailments and puerperal diseases can be attributed to its antibacterial property.
Department of Biotechnology, Indian Institute of Technology Madras, Chennai 600036, TN, India.
P S
PS
Pavithra
N
N
Sreevidya
Rama S
RS
Verma
D000900:Anti-Bacterial Agents / Q000032:analysis / Q000494:pharmacology*; D001419:Bacteria / Q000187:drug effects*; D005054:Eugenol / Q000032:analysis / Q000494:pharmacology; D005233:Fatty Alcohols / Q000032:analysis / Q000494:pharmacology; D039821:Monoterpenes / Q000032:analysis / Q000494:pharmacology; D009822:Oils, Volatile / Q000032:analysis / Q000494:pharmacology*; D019308:Palmitic Acid / Q000032:analysis / Q000494:pharmacology; D010936:Plant Extracts / Q000032:analysis / Q000737:chemistry / Q000494:pharmacology*; D018515:Plant Leaves; D027821:Rutaceae / Q000737:chemistry*
D000900:Anti-Bacterial Agents; D005233:Fatty Alcohols; D039821:Monoterpenes; D009822:Oils, Volatile; D010936:Plant Extracts; D019308:Palmitic Acid; D005054:Eugenol; C051045:1-tridecanol