Computational gene annotation in new genome assemblies using GeneID.
The sequence of many eukaryotic genomes is nowadays available from a personal computer to any researcher in the world-wide scientific community. However, the sequences are worthless without the adequate annotation of the biological meaningful elements. The annotation of the genes, in particular, is a challenging task that can not be tackled without the aid of specific bioinformatics tools. We present in this chapter a simple protocol mainly based on the combination of the program GeneID and other computational tools to annotate the location of a gene, which was previously annotated in D. melanogaster, in the recently assembled genome of D. yakuba.
Departament de Genètica, Facultat de Biologia, Universitat de Barcelona, Spain.
Enrique
E
Blanco
Josep F
JF
Abril
D000595:Amino Acid Sequence; D001483:Base Sequence; D030541:Databases, Genetic; D005796:Genes; D016678:Genome; D023281:Genomics / Q000379:methods*; D008969:Molecular Sequence Data; D016415:Sequence Alignment / Q000379:methods; D012984:Software