Floral stem cell termination involves the direct regulation of AGAMOUS by PERIANTHIA.
In Arabidopsis, the population of stem cells present in young flower buds is lost after the production of a fixed number of floral organs. The precisely timed repression of the stem cell identity gene WUSCHEL (WUS) by the floral homeotic protein AGAMOUS (AG) is a key part of this process. In this study, we report on the identification of a novel input into the process of floral stem cell regulation. We use genetics and chromatin immunoprecipitation assays to demonstrate that the bZIP transcription factor PERIANTHIA (PAN) plays a role in regulating stem cell fate by directly controlling AG expression and suggest that this activity is spatially restricted to the centermost region of the AG expression domain. These results suggest that the termination of floral stem cell fate is a multiply redundant process involving loci with unrelated floral patterning functions.
Das
Pradeep
P
California Institute of Technology, Pasadena, California 91125, USA. pradeep.das@ens-lyon.fr
Ito
Toshiro
T
Wellmer
Frank
F
Vernoux
Teva
T
Dedieu
Annick
A
Traas
Jan
J
Meyerowitz
Elliot M
EM
D026342:AGAMOUS Protein, Arabidopsis / Q000235:genetics / Q000378:metabolism*; D017360:Arabidopsis / Q000166:cytology* / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D019521:Body Patterning / Q000502:physiology; D002454:Cell Differentiation / Q000502:physiology; D004268:DNA-Binding Proteins / Q000235:genetics / Q000502:physiology*; D035264:Flowers / Q000166:cytology* / Q000502:physiology; D018506:Gene Expression Regulation, Plant; D018398:Homeodomain Proteins / Q000378:metabolism; D009154:Mutation; D013234:Stem Cells / Q000166:cytology* / Q000502:physiology; D014157:Transcription Factors / Q000235:genetics / Q000502:physiology*
D026342:AGAMOUS Protein, Arabidopsis; D029681:Arabidopsis Proteins; D004268:DNA-Binding Proteins; D018398:Homeodomain Proteins; C118078:PAN protein, Arabidopsis; D014157:Transcription Factors; C116421:WUSCHEL protein, Arabidopsis