Isolation and characteristics of a novel biphenyl-degrading bacterial strain, Dyella ginsengisoli LA-4.
A novel biphenyl-degrading bacterial strain LA-4 was isolated from activated sludge. It was identified as Dyella ginsengisoli according to phylogenetic similarity of 16S rRNA gene sequence. This isolate could utilize biphenyl as sole source of carbon and energy, which degraded over 95 mg/L biphenyl within 36 h. The major metabolites formed from biphenyl, such as 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid (HOPDA) and benzoic acid, were identified by LC-MS. The crude cell extract of strain LA-4 exhibited the activity of 2,3-dihydroxybiphenyl 1,2-dioxygenase (2,3-DHBD) and the kinetic parameters K(m) was 26.48 micromol/L and V(max) was 8.12 U/mg protein. A conserved region of the biphenyl dioxygenase gene bphA1 of strain LA-4 was amplified by PCR and confirmed by DNA sequencing.
Li
Ang
A
School of Environmental and Biological Science and Technology, Key Laboratory of Industrial Ecology and Environmental Engineering (Ministry of Education, China), Dalian University of Technology, Dalian 116024, China. li_ang332@163.com
Qu
Yuanyuan
Y
Zhou
Jiti
J
Gou
Min
M
D001419:Bacteria / Q000201:enzymology / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification*; D001673:Biodegradation, Environmental; D001713:Biphenyl Compounds / Q000737:chemistry / Q000378:metabolism*; D002396:Catechols / Q000737:chemistry; D003001:Cloning, Molecular; D049308:Dioxygenases / Q000235:genetics; D004587:Electrophoresis, Agar Gel; D005798:Genes, Bacterial; D007700:Kinetics; D010802:Phylogeny; D013056:Spectrophotometry, Ultraviolet; D013997:Time Factors
D001713:Biphenyl Compounds; D002396:Catechols; D049308:Dioxygenases; C454813:2,3-dihydroxybiphenyl; C010574:biphenyl; C074272:2,3-dihydroxybiphenyl oxygenase