title: Comparison of enantioselective HPLC separation of structurally diverse compounds on chiral stationary phases with different teicoplanin coverage and distinct linkage chemistry.
abstract: Enantioseparation performance of two teicoplanin-based chiral stationary phases, Chirobiotic T and Chirobiotic T2, with different teicoplanin coverage and distinct linkage chemistry was compared. Three structurally diverse groups of analytes, amino alcohols (beta-blockers), chlorophenoxypropionic acids, and branched-chain amino acids, in various mobile phase compositions/separation modes were examined. The amino alcohols showed better enantioresolution on Chirobiotic T2 in reverse-phase, hydrophilic interaction chromatography, and polar-ionic mode separation systems. The best results with these analytes were obtained in the polar-ionic mobile phase. In contrast, the chlorophenoxypropionic acids and the branched-chain amino acids yielded an improved enantioresolution on the chiral stationary phase with lower amount of teicoplanin in the hydrophilic interaction chromatography system. The comparison of separation of the chlorophenoxypropionic acids enantiomers in the reverse-phase and hydrophilic interaction chromatography environments showed completely opposite results. While better enantioresolution of chlorophenoxypropionic acids was achieved on Chirobiotic T2 in mobile phases with low methanol content, high methanol concentration was needed to reach baseline enantioseparation on Chirobiotic T.
authors.affiliation: Department of Analytical Chemistry, Faculty of Science, Charles University in Prague, Hlavova, Prague, Czech Republic.
authors.forename: Marie
authors.initials: M
authors.lastname: Honetschlägerová-Vadinská
authors.forename: Simona
authors.initials: S
authors.lastname: Srkalová
authors.forename: Zuzana
authors.initials: Z
authors.lastname: Bosáková
authors.forename: Pavel
authors.initials: P
authors.lastname: Coufal
authors.forename: Eva
authors.initials: E
authors.lastname: Tesarová
mesh_terms: D000596:Amino Acids / Q000737:chemistry / Q000302:isolation & purification*; D000605:Amino Alcohols / Q000737:chemistry / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D015394:Molecular Structure; D011422:Propionates / Q000737:chemistry / Q000302:isolation & purification*; D013237:Stereoisomerism; D017334:Teicoplanin / Q000737:chemistry*; D013997:Time Factors
chemical_list: D000596:Amino Acids; D000605:Amino Alcohols; D011422:Propionates; D017334:Teicoplanin