title: Acute high-dose sodium selenite administration improves intestinal microcirculation without affecting cytokine release in experimental endotoxemia.
abstract: We evaluated the effects of acute high-dose sodium selenite (SEL) administration on the intestinal microcirculation and the release of the cytokines TNF-alpha, IL-1beta, IL-6 and IL-10 in experimental endotoxemia (induced by lipopolysaccharide-LPS). Three groups of animals (n=30) were studied: control group, endotoxemic group (15 mg kg(-1) i.v. LPS from E. coli) and SEL-treated LPS group (100 microg kg(-1) SEL i.v.). SEL treatment resulted in a significant reduced number of firmly adhering leukocytes in intestinal submucosal venules and reduced significantly the impairment of the intestinal functional capillary density. Despite of the improvement of microcirculatory parameters, we did not detect any changes in the pattern of cytokine release. In conclusion, administration of high-dose sodium SEL attenuates leukocyte adhesion and improves capillary perfusion within the intestinal microcirculation without affecting release of the cytokines TNF-alpha, IL-1beta, IL-6 and IL-10 in experimental endotoxemia.
authors.affiliation: Klinik und Poliklinik für Anästhesiologie und Intensivmedizin, Ernst-Moritz- Arndt-Universität, Greifswald, Germany.
authors.forename: Matthias
authors.initials: M
authors.lastname: Gründling
authors.forename: Sandra
authors.initials: S
authors.lastname: Rickert
authors.forename: Daniela
authors.initials: D
authors.lastname: Saeger
authors.forename: Dragan
authors.initials: D
authors.lastname: Pavlovic
authors.forename: Taras
authors.initials: T
authors.lastname: Usichenko
authors.forename: Konrad
authors.initials: K
authors.lastname: Meissner
authors.forename: Michael
authors.initials: M
authors.lastname: Wendt
authors.forename: Orlando
authors.initials: O
authors.lastname: Hung
authors.forename: Michael
authors.initials: M
authors.lastname: Murphy
authors.forename: Sara
authors.initials: S
authors.lastname: Whynot
authors.forename: Christian
authors.initials: C
authors.lastname: Lehmann
mesh_terms: D000818:Animals; D002448:Cell Adhesion; D016207:Cytokines / Q000378:metabolism*; D019446:Endotoxemia / Q000378:metabolism*; D016753:Interleukin-10 / Q000378:metabolism; D015850:Interleukin-6 / Q000378:metabolism; D007413:Intestinal Mucosa / Q000378:metabolism; D007422:Intestines / Q000098:blood supply*; D007962:Leukocytes / Q000187:drug effects / Q000378:metabolism; D008297:Male; D008833:Microcirculation / Q000187:drug effects; D051381:Rats; D011917:Rats, Inbred Lew; D018038:Sodium Selenite / Q000008:administration & dosage* / Q000494:pharmacology; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D016207:Cytokines; D015850:Interleukin-6; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10; D018038:Sodium Selenite