Characterisation of RC-proteoliposomes at different RC/lipid ratios.
Reconstitution of membrane proteins in phospholipid vesicles allows the investigation of such macromolecules in a biomimetic simplified environment. The often employed micelle-to-vesicle-transition method for proteoliposome preparation is a fast and reproducible technique. In this, communication is shown that the lipid/protein ratio influences the size of the proteoliposomes and the actual protein reconstitution. The results indicate that for photosynthetic reaction centres, the best conditions for ligand-interaction experiments are achieved with a lipid/protein value of 1000:1, while for complete protein incorporation, the 2000:1 ratio should be chosen.
Milano
Francesco
F
CNR, Istituto per i Processi Chimico-Fisici, Sezione di Bari, c/o Dipartimento di Chimica, Via Orabona, 4 I-70124, Bari, Italy.
Italiano
Francesca
F
Agostiano
Angela
A
Trotta
Massimo
M
D007700:Kinetics; D008027:Light; D008055:Lipids / Q000032:analysis / Q000737:chemistry*; D008823:Micelles; D010713:Phosphatidylcholines; D045322:Photosynthetic Reaction Center Complex Proteins / Q000737:chemistry*; D011510:Proteolipids / Q000737:chemistry*; D012542:Scattering, Radiation; D017550:Spectroscopy, Fourier Transform Infrared
D008055:Lipids; D008823:Micelles; D010713:Phosphatidylcholines; D045322:Photosynthetic Reaction Center Complex Proteins; D011510:Proteolipids; C020485:proteoliposomes; C028694:1-palmitoyl-2-oleoylphosphatidylcholine