title: BS69 negatively regulates the canonical NF-kappaB activation induced by Epstein-Barr virus-derived LMP1.
abstract: Epstein-Barr virus (EBV) latent membrane protein 1 (LMP1) activates NF-kappaB signaling pathways through the two C-terminal regions, CTAR1 and CTAR2. BS69 has previously been shown to be involved in LMP1-induced c-Jun N-terminal kinase activation through CTAR2 by interacting with tumor necrosis factor (TNFR) receptor-associated factor 6. In the present study, our manipulation of BS69 expression clearly indicates that BS69 negatively regulates LMP1-mediated NF-kappaB activation and up-regulates IL-6 mRNA expression and IkappaB degradation. Our immunoprecipitation experiments suggest that BS69 decreases complex formation between LMP1 and TNFR-associated death domain protein (TRADD).
authors.affiliation: Department of Immunology, Graduate School of Pharmaceutical Sciences, Hokkaido University, Kita-Ku Kita 12 Nishi 6, Sapporo, Japan.
authors.forename: Osamu
authors.initials: O
authors.lastname: Ikeda
authors.forename: Yuichi
authors.initials: Y
authors.lastname: Sekine
authors.forename: Akihiro
authors.initials: A
authors.lastname: Mizushima
authors.forename: Kenji
authors.initials: K
authors.lastname: Oritani
authors.forename: Teruhito
authors.initials: T
authors.lastname: Yasui
authors.forename: Masahiro
authors.initials: M
authors.lastname: Fujimuro
authors.forename: Ryuta
authors.initials: R
authors.lastname: Muromoto
authors.forename: Asuka
authors.initials: A
authors.lastname: Nanbo
authors.forename: Tadashi
authors.initials: T
authors.lastname: Matsuda
mesh_terms: D002352:Carrier Proteins / Q000032:analysis / Q000378:metabolism*; D018797:Cell Cycle Proteins; D002478:Cells, Cultured; D056970:Co-Repressor Proteins; D004268:DNA-Binding Proteins; D005786:Gene Expression Regulation; D006367:HeLa Cells; D006801:Humans; D015850:Interleukin-6 / Q000235:genetics / Q000378:metabolism; D008856:Microscopy, Fluorescence; D008954:Models, Biological; D016328:NF-kappa B / Q000032:analysis / Q000378:metabolism*; D015398:Signal Transduction; D053199:TNF Receptor-Associated Death Domain Protein / Q000378:metabolism; D014763:Viral Matrix Proteins / Q000032:analysis / Q000378:metabolism*
chemical_list: D002352:Carrier Proteins; D018797:Cell Cycle Proteins; D056970:Co-Repressor Proteins; D004268:DNA-Binding Proteins; C037662:EBV-associated membrane antigen, Epstein-Barr virus; D015850:Interleukin-6; D016328:NF-kappa B; D053199:TNF Receptor-Associated Death Domain Protein; D014763:Viral Matrix Proteins; C094763:ZMYND11 protein, human