Evaluation of antioxidant and anti-atherogenic properties of Glycyrrhiza glabra root using in vitro models.
The aim of present study was to evaluate antioxidant property of Glycyrrhiza glabra root extracts using in vitro models. The dose-dependent aqueous and ethanolic extracts demonstrated the scavenging activity against nitric oxide (concentration that caused 50% inhibition of nitric oxide radicals [IC(50)]=72 and 62.1 microg/ml, respectively), superoxide (IC(50)=64.2 and 38.4 microg/ml, respectively), hydroxyl (IC(50)=81.9 and 63 microg/ml, respectively), DPPH (IC(50)=43.6 and 28.3 microg/ml, respectively) and ABTS(*+) (IC(50)=77.3 and 57.2 microg/ml, respectively) radicals. Further, both extracts showed strong reducing power and iron-chelating capacities. In the Fe(2+)/ascorbate system, both extracts were found to inhibit mitochondrial fraction lipid peroxidation. In copper-catalyzed human serum and low-density lipoprotein oxidation models, both extracts significantly (P<0.05) lengthened the lag phase along with a decline in the oxidation rate, conjugated dienes, lipid hydroperoxides and thiobarbituric acid reactive substance formation. In conclusion, ethanolic extract of G. glabra possess considerable antioxidant activity and protective effect against the human lipoprotein oxidative system.
Visavadiya
Nishant P
NP
BRD School of Biosciences, Sardar Patel Maidan, Sardar Patel University, Vallabh Vidyanagar, Gujarat, India. nishantpv@gmail.com
Soni
Badrish
B
Dalwadi
Nirav
N
D000975:Antioxidants / Q000032:analysis* / Q000494:pharmacology; D050197:Atherosclerosis / Q000517:prevention & control*; D002317:Cardiovascular Agents / Q000032:analysis* / Q000494:pharmacology; D002614:Chelating Agents / Q000032:analysis / Q000494:pharmacology; D003300:Copper / Q000737:chemistry; D004305:Dose-Response Relationship, Drug; D016166:Free Radical Scavengers / Q000032:analysis / Q000494:pharmacology; D006035:Glycyrrhiza / Q000737:chemistry*; D020128:Inhibitory Concentration 50; D007501:Iron / Q000737:chemistry; D015227:Lipid Peroxidation / Q000187:drug effects*; D008077:Lipoproteins, LDL / Q000097:blood*; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism; D010084:Oxidation-Reduction; D010936:Plant Extracts / Q000494:pharmacology*; D018517:Plant Roots / Q000737:chemistry; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
D000975:Antioxidants; D002317:Cardiovascular Agents; D002614:Chelating Agents; D016166:Free Radical Scavengers; D008077:Lipoproteins, LDL; D010936:Plant Extracts; D017392:Thiobarbituric Acid Reactive Substances; C099252:oxidized low density lipoprotein; D003300:Copper; D007501:Iron