[Analysis of genetic difference among Scrophularia ningpoensis cultivars by SRAP].
OBJECTIVE
The genetic difference among Scrophularia ningpoensis cultivars were analyzed in molecular level.


METHODS
Ninety-two individuls of three S. ningpoensis cultivars were employed to be analyzed by the approach of Sequence-related Amplified Polymorphism (SRAP).The parameters were calculated by POPGENE1.31 and the relationship was constructed based on UPGMA method.


RESULTS
1) A total of 227 bands were scored and 199 bands of them were polymorphic. 2) The result is showed that there is a medium level of genetic diversity among three cultivars. At species level: percentage of polymorphic loci PPB=52.42%, effective number of alleles N(e)=1.2812, Nei's gene diversity H=0.1671 and Shannon's information index H(sp)= 0.2526; At cultivar level: PPB=21.44%, N(e)=1.1216, Nei's gene diversity H=0.0725 and Shannon's information index H(pop)= 0.1083. 3) The Nei's coefficient of genetic differentiation was 0.5625, which was consistent with the Shannon's coefficient of genetic differentiation (0.5713). Most of the genetic variation existed among cultivars. 4) The gene flow (N(m)=0.3889) was less among cultivars, indicating that the degree of genetic differentiation was higher. 5) Genetic similarity coefficient were changed from 0.8082 to 0.9133. By clustering analysis, the classified result of SRAP marker between traditional modal character was almost same.


CONCLUSIONS
The genetic diversity of samples of S. ningpoensis is medium. The genetic difference among cultivar is higher than that within cultivar.
Chen
Daxia
D
Chongqing Academy of Chinese Materia Medica, Chongqing 400065, China. chendaxia6891@hotmail.com
Li
Longyun
L
Peng
Rui
R
Wu
Yekuan
Y
Cai
Yingfan
Y
D016000:Cluster Analysis; D017931:DNA Primers / Q000235:genetics; D051456:Gene Flow; D005819:Genetic Markers / Q000235:genetics; D014644:Genetic Variation; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D032266:Scrophularia / Q000145:classification* / Q000235:genetics*; D012984:Software
D017931:DNA Primers; D005819:Genetic Markers