title: High-throughput analysis of serum antigens using sandwich ELISAs on microarrays.
abstract: Enzyme-linked immunosorbent assay (ELISA) microarrays promise to be a powerful tool for the detection and validation of disease biomarkers. ELISA microarrays are capable of simultaneous detection of many proteins using a small sample volume. Although there are many potential pitfalls to the use of ELISA microarrays, these can be avoided by careful planning of experiments. In this chapter we describe a high-throughput protocol for processing ELISA microarrays that will result in reliable and reproducible data.
authors.affiliation: Biological Sciences Division, Pacific Northwest National Laboratory, Richland, WA, USA.
authors.forename: Shannon L
authors.initials: SL
authors.lastname: Servoss
authors.forename: Rachel
authors.initials: R
authors.lastname: Gonzalez
authors.forename: Susan
authors.initials: S
authors.lastname: Varnum
authors.forename: Richard C
authors.initials: RC
authors.lastname: Zangar
mesh_terms: D000941:Antigens / Q000097:blood*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006801:Humans; D040081:Protein Array Analysis / Q000379:methods*
chemical_list: D000941:Antigens