Moonlighting protein in Starkeyomyces koorchalomoides: characterization of dihydrolipoamide dehydrogenase as a protein acetyltransferase utilizing acetoxycoumarin as the acetyl group donor.
In this report we have identified for the first time a transacetylase (TAase) in a mesophilic fungi Starkeyomyces koorchalomoides catalyzing the transfer of acetyl group from polyphenolic acetate (PA) to a receptor protein glutathione S-transferase (GST). An elegant assay procedure was established for TAase based on its ability to mediate inhibition of GST by 7,8-diacetoxy-4-methylcoumarin (DAMC), a model PA. Utilizing this assay procedure, S. koorchalomoides TAase was purified to homogeneity. TAase was found to have MW of 50 kDa. The purified enzyme exhibited maximum activity at 45 degrees C at pH 6.8. The N-terminal sequence of purified fungal TAase (ANDASTVED) showed identity with corresponding N-terminal sequence of dihydrolipoamide dehydrogenase (LADH), a mitochondrial matrix enzyme and an E3 component of pyruvate dehydrogenase complex (PDHC). TAase was found to have all the properties of LADH and avidly interacted with the anti-LADH antibody. TAase catalyzed acetylation of GST by DAMC was identified by LC-MS/MS and a single lysine residue (Lys-113) was found to be acetylated. Further, recombinant LADH from Streptococcus pneumoniae lacking lipoyl domain was found to exhibit little TAase activity, suggesting the role of lipoyl domain in the TAase activity of LADH. These observations bear evidence for the protein acetyltransferase activity of LADH. Such an activity of LADH can be attributed as a moonlighting function of the enzyme.
Tyagi
Tapesh K
TK
Department of Biochemistry, V. P. Chest Institute, University of Delhi, Delhi 110007, India.
Ponnan
Prija
P
Singh
Prabhjot
P
Bansal
Seema
S
Batra
Anoop
A
Collin
Fabrice
F
Guillonneau
Francois
F
Jore
Daniel
D
Patkar
Shamkant A
SA
Saxena
Rajendra K
RK
Parmar
Virinder S
VS
Rastogi
Ramesh C
RC
Raj
Hanumantharao G
HG
D000107:Acetylation; D000123:Acetyltransferases / Q000737:chemistry* / Q000235:genetics; D003374:Coumarins / Q000737:chemistry; D008058:Dihydrolipoamide Dehydrogenase / Q000737:chemistry* / Q000235:genetics; D005658:Fungi / Q000201:enzymology*; D005982:Glutathione Transferase / Q000737:chemistry; D013296:Streptococcus pneumoniae / Q000201:enzymology
C114369:7,8-diacetoxy-4-methylcoumarin; D003374:Coumarins; D008058:Dihydrolipoamide Dehydrogenase; D000123:Acetyltransferases; D005982:Glutathione Transferase