title: Critical role of TRPC6 channels in VEGF-mediated angiogenesis.
abstract: Intracellular Ca(2+) signaling plays critical roles in VEGF-mediated angiogenesis. Transient receptor potential canonical (TRPC) channel 6, a Ca(2+)-permeable non-selective cation channel, can be activated by VEGF. Here, we report that TRPC6 is important for VEGF-mediated angiogenesis. Inhibition of TRPC6 in human umbilical vein endothelial cells (HUVECs) by pharmacological or genetic approaches arrested HUVECs at G2/M phase and suppressed VEGF-induced HUVEC proliferation and tube formation. Furthermore, inhibition of TRPCs abolished VEGF-, but not FGF-induced angiogenesis in the chick embryo chorioallantoic membrane. These results suggest that TRPC6 plays an important role in VEGF-mediated angiogenesis.
authors.affiliation: Division of Comprehensive Treatment, Eastern Hepatobiliary Hospital, Second Military Medical University, Shanghai 200438, China.
authors.forename: Ruiliang
authors.initials: R
authors.lastname: Ge
authors.forename: Yilin
authors.initials: Y
authors.lastname: Tai
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Sun
authors.forename: Kechun
authors.initials: K
authors.lastname: Zhou
authors.forename: Shenglian
authors.initials: S
authors.lastname: Yang
authors.forename: Tianlin
authors.initials: T
authors.lastname: Cheng
authors.forename: Qifei
authors.initials: Q
authors.lastname: Zou
authors.forename: Feng
authors.initials: F
authors.lastname: Shen
authors.forename: Yizheng
authors.initials: Y
authors.lastname: Wang
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002453:Cell Cycle; D002642:Chick Embryo; D049033:Chorioallantoic Membrane / Q000098:blood supply / Q000378:metabolism; D042783:Endothelial Cells / Q000378:metabolism*; D006801:Humans; D018919:Neovascularization, Physiologic / Q000502:physiology*; D018408:Patch-Clamp Techniques; D020133:Reverse Transcriptase Polymerase Chain Reaction; D050052:TRPC Cation Channels / Q000378:metabolism*; D000074287:TRPC6 Cation Channel; D014471:Umbilical Veins / Q000166:cytology / Q000378:metabolism; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism*
chemical_list: D050052:TRPC Cation Channels; D000074287:TRPC6 Cation Channel; C117032:TRPC6 protein, human; D042461:Vascular Endothelial Growth Factor A