In vivo sensitivity of blue-light-dependent signaling mediated by AppA/PpsR or PrrB/PrrA in Rhodobacter sphaeroides.
Formation of photosynthesis complexes in Rhodobacter sphaeroides is regulated in a redox- and light-dependent manner by the AppA/PpsR and PrrB/PrrA systems. While on the one hand, blue light is sensed by the flavin adenine dinucleotide-binding BLUF domain of AppA, on the other, light is absorbed by bacteriochlorophyll signals through PrrB/PrrA. We show that much smaller quantities initiate the AppA-mediated response to blue light than the bacteriochlorophyll-mediated response.
Metz
Sebastian
S
Institut für Mikro- und Molekularbiologie, University of Giessen, Giessen, Germany.
Jäger
Andreas
A
Klug
Gabriele
G
D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D015152:Blotting, Northern; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D005420:Flavoproteins / Q000235:genetics / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial / Q000528:radiation effects*; D055537:Light Signal Transduction / Q000502:physiology*; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012242:Rhodobacter sphaeroides / Q000235:genetics / Q000378:metabolism* / Q000528:radiation effects
C116785:AppA protein, Rhodobacter sphaeroides; D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; D005420:Flavoproteins; D012097:Repressor Proteins