Tea polyphenols exerts anti-hepatitis B virus effects in a stably HBV-transfected cell line.
In this study, the anti-HBV effects of tea polyphenols (TP) were examined. After cells were exposed to TP for 3, 6, 9 days, amounts of HBsAg, HBeAg and HBV-DNA released into the supernatant of the cultured HepG2 2.2.15 cells were detected. TP, to some extent, inhibited the secretion of HBsAg and strongly suppressed the secretion of HBeAg in a dose-dependent (P<0.01) and time-dependent manner, with 50% maximal inhibitory concentration (IC50) value being 7.34 microg/mL on the 9th day, but the time-dependence was not significant (P=0.051). Expression of HBV-DNA in the supernatant of the cell culture also was significantly decreased in a dose-dependent fashion (P<0.01). The IC50 of TP in inhibiting HBV DNA was 2.54 microg/mL. It concluded that TP possessed potential anti-HBV effects and may be used as a treatment alternative for HBV infection.
Ye
Pian
P
Department of Hepatology and Infectious Diseases, Huazhong University of Science and Technology, Wuhan, 430022, China. yp_breezy@yahoo.com.cn
Zhang
Shuling
S
Zhao
Lei
L
Dong
Jihua
J
Jie
Shenghua
S
Pang
Ran
R
Li
Shuli
S
D000998:Antiviral Agents / Q000494:pharmacology*; D004279:DNA, Viral / Q000032:analysis; D004305:Dose-Response Relationship, Drug; D005419:Flavonoids / Q000494:pharmacology*; D056945:Hep G2 Cells; D006514:Hepatitis B Surface Antigens / Q000032:analysis; D006513:Hepatitis B e Antigens / Q000032:analysis; D006515:Hepatitis B virus / Q000187:drug effects*; D006801:Humans; D020128:Inhibitory Concentration 50; D010636:Phenols / Q000494:pharmacology*; D059808:Polyphenols; D013662:Tea / Q000737:chemistry*
D000998:Antiviral Agents; D004279:DNA, Viral; D005419:Flavonoids; D006514:Hepatitis B Surface Antigens; D006513:Hepatitis B e Antigens; D010636:Phenols; D059808:Polyphenols; D013662:Tea