[Immune resonance scattering spectral analysis of fenvalerate].
In the pH 7.2 Na2HPO4-NaH2PO4 buffer solutions and in the presence of PEG-6000, fenvalerate (Fen) antisera was combined with Fen specifically, and aggregated to form immune complex particles that exhibited five resonance scattering peaks at 350, 390, 420, 440 and 480 nm respectively. The peak at 390 nm was the strongest and was chosen for use. Fen concentration (c) in the range of 0.20 to 6.40 microg x mL(-1) was proportional to the resonance scattering intensity at 390 nm. Its regression equation was DeltaIRS 23.05c-1.39, the correlation coefficient was 0.9978, and the detection limit was 0.07 microg x mL(-1). Effects of buffer solution type, pH value, buffer solution volume, fenvalerate antisera concentration, PEG-6000 concentration, incubation temperature and time on the resonance scattering intensity were considered in detail. With pH (5.8-8.0) increasing, the IRS and Ib all decreased. When the pH value was at 7.2, the DeltaIRS was bigger. Three buffer solutions of pH 7.2, including Na2HPO4-citric acid, Na2HPO4-KH2PO4 and Na2HPO4-NaH2PO4, were examined. The pH 7.2 Na2HPO4-NaH2PO4 buffer solution gives the biggest DeltaIRS value. PEG-6000 could enhance the DeltaIRS value. When the concentration of PEG-6000 was 50.0 mg x mL(-1), the DeltaIRS was achieved at max. Fen was a stable chemical. The IRS increased within 20 min,while the DeltaIRS remained constant when incubation time was in the range of 20-40 min. The condition of a pH 7.2 Na2HPO4-NaH2PO4 buffer solution-50.0 mg x mL(-1) PEG-6000-6.67 microg x mL(-1) Fen antisera-30 degrees C-incubation time 20 min was chosen. According to the procedure, the influence of foreign substances on the determination of 1.60 microg x mL(-1) Fen was examined, within a relative error of +/- 5%. Results showed that the following coexistent substances had no impact on the RS assay: 96 microg x mL(-1) ametryne, 96 microg x mL(-1) m-aminotoluene, 48 microg x mL(-1) simetryne, 48 microg x mL(-1) p-aminotoluene,80 microg x mL(-1) BSA, 80 microg x mL(-1) HSA, 80 microg x mL(-1) Fe3+, 80 microg x mL(-1) Mg2+, 160 microg x mL(-1) Ca2+, and 160 microg x mL(-1) glucose. The results indicated that this RSS assay has good selectivity. This immune resonance scattering spectral assay was applied to the determination of Fen in waste water samples with satisfactory results. The recovery was in the range of 92.91%-101.25%, and the relative standard deviation was in the range of 1.71%-4.80%.
Ma
Wen-Sheng
WS
School of Environment and Resource, Guangxi Normal University, Guilin 541004, China.
Wang
Su-Mei
SM
Jiang
Zhi-Liang
ZL
D002021:Buffers; D002118:Calcium / Q000032:analysis; D002384:Catalysis; D004791:Enzyme Inhibitors / Q000032:analysis*; D057230:Limit of Detection; D008274:Magnesium / Q000032:analysis; D053768:Metal Nanoparticles; D009570:Nitriles / Q000032:analysis*; D011188:Potassium / Q000032:analysis; D011722:Pyrethrins / Q000032:analysis*; D012542:Scattering, Radiation; D012964:Sodium / Q000032:analysis
D002021:Buffers; D004791:Enzyme Inhibitors; D009570:Nitriles; D011722:Pyrethrins; D012964:Sodium; D008274:Magnesium; D011188:Potassium; D002118:Calcium; C017690:fenvalerate