Leukotriene B4 regulates proliferation and differentiation of cultured rat myoblasts via the BLT1 pathway.
Skeletal muscle regeneration is a highly orchestrated process initiated by activation of adult muscle satellite cells. Upon muscle injury, the inflammatory process is always accompanied by muscle regeneration. Leukotriene B(4) is one of the essential inflammatory mediators. We isolated and cultured primary satellite cells. RT-PCR showed that myoblasts expressed mRNA for LTB(4) receptors BLT1 and BLT2, and LTB4 promoted myoblast proliferation and fusion. Quantitative real-time PCR and immunoblotting showed that LTB(4) treatment expedited the expression process of differentiation markers MyoD and M-cadherin. U-75302, a specific BLT1 inhibitor, but not LY2552833, a specific BLT2 inhibitor, blocked proliferation and differentiation of myoblasts induced by LTB(4), which implies the involvement of the BLT1 pathway. Overall, the data suggest that LTB(4) contributes to muscle regeneration by accelerating proliferation and differentiation of satellite cells.
Sun
Ru
R
Institute of Genetics and Cytology, Northeast Normal University, Changchun, China.
Ba
Xueqing
X
Cui
Lingling
L
Xue
Yan
Y
Zeng
Xianlu
X
D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects / Q000502:physiology; D048708:Cell Growth Processes / Q000187:drug effects / Q000502:physiology; D002478:Cells, Cultured; D005233:Fatty Alcohols / Q000494:pharmacology; D005455:Fluorescent Antibody Technique; D006018:Glycols / Q000494:pharmacology; D020024:Leukotriene Antagonists / Q000494:pharmacology; D007975:Leukotriene B4 / Q000378:metabolism* / Q000494:pharmacology; D032446:Myoblasts / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D051381:Rats; D018102:Receptors, Leukotriene B4 / Q000096:biosynthesis* / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D032496:Satellite Cells, Skeletal Muscle / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects; D013777:Tetrazoles / Q000494:pharmacology
D005233:Fatty Alcohols; D006018:Glycols; D020024:Leukotriene Antagonists; D012333:RNA, Messenger; D018102:Receptors, Leukotriene B4; D013777:Tetrazoles; C062137:U 75302; D007975:Leukotriene B4; C063823:LY 255283