title: Controlled synthesis of CdSe and CdSe/CdS core/shell nanoparticles using Gemini surfactant Py-16-10-16 and their bioconjugates with BSA.
abstract: CdSe and CdSe/CdS core/shell nanoparticles (NPs) were synthesized by using Gemini surfactant, 1,10-bis(alpha-hexadecyl pyridine) decamethylene dibromide (abbreviated Py-16-10-16), as a soft template. Subsequent analysis revealed that the as-synthesized CdSe NPs and CdSe/CdS core/shell NPs were highly luminescent with quantum yields of 18% and 35%, respectively, compared with Rhodamine 6G (95%). Furthermore, when the nanoparticles were conjugated with bovine serum albumin (BSA) in PBS buffer solution (pH 7.40), the fluorescence intensity of the BSA system was quenched by the addition of CdSe/TGA or CdSe/CdS/TGA NPs, while the fluorescence intensities of CdSe/TGA and CdSe/CdS/TGA NPs were enhanced by the addition of BSA. This enhancement and quenching of the fluorescence intensity by conjugating with BSA could be used to determine the albumin concentration.
authors.affiliation: School of Chemistry and Chemical Engineering, Anhui University, Hefei 230039, PR China.
authors.forename: Wengui
authors.initials: W
authors.lastname: Chang
authors.forename: Yuhua
authors.initials: Y
authors.lastname: Shen
authors.forename: Anjian
authors.initials: A
authors.lastname: Xie
authors.forename: Hui
authors.initials: H
authors.lastname: Zhang
authors.forename: Juan
authors.initials: J
authors.lastname: Wang
authors.forename: Wensheng
authors.initials: W
authors.lastname: Lu
mesh_terms: D019187:Cadmium Compounds / Q000138:chemical synthesis* / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy; D053768:Metal Nanoparticles; D008855:Microscopy, Electron, Scanning; D018036:Selenium Compounds / Q000138:chemical synthesis* / Q000737:chemistry; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D017550:Spectroscopy, Fourier Transform Infrared; D013440:Sulfides / Q000138:chemical synthesis* / Q000737:chemistry; D013501:Surface-Active Agents / Q000737:chemistry*; D014961:X-Ray Diffraction
chemical_list: D019187:Cadmium Compounds; D018036:Selenium Compounds; D013440:Sulfides; D013501:Surface-Active Agents; C034939:cadmium sulfide; D012710:Serum Albumin, Bovine; C058667:cadmium selenide