title: Transcriptional network of p63 in human keratinocytes.
abstract: p63 is a transcription factor required for the development and maintenance of ectodermal tissues in general, and skin keratinocytes in particular. The identification of its target genes is fundamental for understanding the complex network of gene regulation governing the development of epithelia. We report a list of almost 1000 targets derived from ChIP on chip analysis on two platforms; all genes analyzed changed in expression during differentiation of human keratinocytes. Functional annotation highlighted unexpected GO terms enrichments and confirmed that genes involved in transcriptional regulation are the most significant. A detailed analysis of these transcriptional regulators in condition of perturbed p63 levels confirmed the role of p63 in the regulatory network. Rather than a rigid master-slave hierarchical model, our data indicate that p63 connects different hubs involved in the multiple specific functions of the skin.
authors.affiliation: Department of Biomolecular Sciences and Biotechnologies, University of Milan, Milan, Italy.
authors.forename: Silvia
authors.initials: S
authors.lastname: Pozzi
authors.forename: Federico
authors.initials: F
authors.lastname: Zambelli
authors.forename: Daniele
authors.initials: D
authors.lastname: Merico
authors.forename: Giulio
authors.initials: G
authors.lastname: Pavesi
authors.forename: Amélie
authors.initials: A
authors.lastname: Robert
authors.forename: Peggy
authors.initials: P
authors.lastname: Maltère
authors.forename: Xavier
authors.initials: X
authors.lastname: Gidrol
authors.forename: Roberto
authors.initials: R
authors.lastname: Mantovani
authors.forename: M Alessandra
authors.initials: MA
authors.lastname: Vigano
mesh_terms: D004475:Ectoderm / Q000254:growth & development; D004848:Epithelium / Q000254:growth & development; D005786:Gene Expression Regulation; D053263:Gene Regulatory Networks / Q000235:genetics*; D006801:Humans; D015603:Keratinocytes / Q000378:metabolism*; D012867:Skin / Q000166:cytology / Q000254:growth & development*; D015534:Trans-Activators / Q000235:genetics* / Q000378:metabolism; D014157:Transcription Factors; D025521:Tumor Suppressor Proteins / Q000235:genetics* / Q000378:metabolism
chemical_list: C115051:TP63 protein, human; D015534:Trans-Activators; D014157:Transcription Factors; D025521:Tumor Suppressor Proteins