Enhanced cerebral expression of MCT1 and MCT2 in a rat ischemia model occurs in activated microglial cells.
Monocarboxylate transporters (MCTs) are essential for the use of lactate, an energy substrate known to be overproduced in brain during an ischemic episode. The expression of MCT1 and MCT2 was investigated at 48 h of reperfusion from focal ischemia induced by unilateral extradural compression in Wistar rats. Increased MCT1 mRNA expression was detected in the injured cortex and hippocampus of compressed animals compared to sham controls. In the contralateral, uncompressed hemisphere, increases in MCT1 mRNA level in the cortex and MCT2 mRNA level in the hippocampus were noted. Interestingly, strong MCT1 and MCT2 protein expression was found in peri-lesional macrophages/microglia and in an isolectin B4+/S100beta+ cell population in the corpus callosum. In vitro, MCT1 and MCT2 protein expression was observed in the N11 microglial cell line, whereas an enhancement of MCT1 expression by tumor necrosis factor-alpha (TNF-alpha) was shown in these cells. Modulation of MCT expression in microglia suggests that these transporters may help sustain microglial functions during recovery from focal brain ischemia. Overall, our study indicates that changes in MCT expression around and also away from the ischemic area, both at the mRNA and protein levels, are a part of the metabolic adaptations taking place in the brain after ischemia.
Moreira
Tiago J T P
TJ
Department of Physiology, University of Lausanne, Lausanne, Switzerland.
Pierre
Karin
K
Maekawa
Fumihiko
F
Repond
Cendrine
C
Cebere
Aleta
A
Liljequist
Sture
S
Pellerin
Luc
L
D000818:Animals; D001921:Brain / Q000378:metabolism / Q000473:pathology*; D001923:Brain Chemistry; D002545:Brain Ischemia; D002540:Cerebral Cortex / Q000378:metabolism / Q000473:pathology; D004734:Energy Metabolism; D005786:Gene Expression Regulation / Q000502:physiology*; D006624:Hippocampus / Q000378:metabolism / Q000473:pathology; D017628:Microglia / Q000378:metabolism* / Q000473:pathology; D027501:Monocarboxylic Acid Transporters / Q000235:genetics* / Q000502:physiology; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017208:Rats, Wistar; D027981:Symporters / Q000235:genetics* / Q000502:physiology; D000099233:Monocarboxylate Transport Protein 1
D027501:Monocarboxylic Acid Transporters; D012333:RNA, Messenger; D027981:Symporters; D000099233:Monocarboxylate Transport Protein 1; C091633:Slc16a7 protein, rat