title: Oceanicola pacificus sp. nov., isolated from a deep-sea pyrene-degrading consortium.
abstract: A taxonomic study was carried out on a Gram-negative, rod-shaped, non-motile, halophilic bacterium, designated strain W11-2B(T), which was isolated from a pyrene-degrading consortium that was enriched from sediment from the Pacific Ocean. Growth was observed at salinities of 0.5-10 % and at temperatures of 10-41 degrees C. Strain W11-2B(T) was unable to degrade Tween 80 or gelatin. 16S rRNA gene sequence comparisons showed that strain W11-2B(T) was related most closely to Oceanicola nanhaiensis SS011B1-20(T) (95.8 % similarity) and Oceanicola batsensis HTCC2597(T) (95.7 %); levels of 16S rRNA gene sequence similarity between strain W11-2B(T) and the type strains of other species tested were below 95.2 %. The dominant fatty acids of strain W11-2B(T) were C(18 : 1)omega7c (32.1 % of the total), C(19 : 0) cyclo (20.9 %), C(18 : 1)omega7c 11-methyl (19.5 %), C(18 : 0) (7.3 %), C(17 : 0) (6.6 %) and C(16 : 0) (3.8 %). The G+C content of the chromosomal DNA was 64.6 mol%. The above data were in good agreement with those of members of the genus Oceanicola. Based on morphology, physiology, fatty acid composition and 16S rRNA gene sequence data, strain W11-2B(T) is considered to represent a novel species of the genus Oceanicola, for which the name Oceanicola pacificus sp. nov. is proposed. The type strain is W11-2B(T) (=CCTCC AB 208224(T)=LMG 24619(T)=MCCC 1A01034(T)).
authors.affiliation: School of Life Sciences, MOE of Key Lab for Subtropical Wetland Ecosystem, Xiamen University, Xiamen 361005, PR China.
authors.forename: Jun
authors.initials: J
authors.lastname: Yuan
authors.forename: Qiliang
authors.initials: Q
authors.lastname: Lai
authors.forename: Baojiang
authors.initials: B
authors.lastname: Wang
authors.forename: Fengqin
authors.initials: F
authors.lastname: Sun
authors.forename: Xiupian
authors.initials: X
authors.lastname: Liu
authors.forename: Yaping
authors.initials: Y
authors.lastname: Du
authors.forename: Guangyu
authors.initials: G
authors.lastname: Li
authors.forename: Li
authors.initials: L
authors.lastname: Gu
authors.forename: Tianling
authors.initials: T
authors.lastname: Zheng
authors.forename: Zongze
authors.initials: Z
authors.lastname: Shao
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D001673:Biodegradation, Environmental; D004269:DNA, Bacterial / Q000032:analysis; D004275:DNA, Ribosomal / Q000032:analysis; D005227:Fatty Acids / Q000032:analysis; D020459:Genes, rRNA; D005838:Genotype; D008969:Molecular Sequence Data; D010140:Pacific Ocean; D010641:Phenotype; D010802:Phylogeny; D011721:Pyrenes / Q000378:metabolism*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D041902:Rhodobacteraceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology; D012623:Seawater / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D013045:Species Specificity
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D011721:Pyrenes; D012336:RNA, Ribosomal, 16S; C030984:pyrene