Multivariate modeling of chromium-induced oxidative stress and biochemical changes in plants of Pistia stratiotes L.
Biochemical changes in the plants of Pistia stratiotes L., a free floating macrophyte exposed to different concentrations of hexavalent chromium (0, 10, 40, 60, 80 and 160 microM) for 48, 96 and 144 h were studied. Chromium-induced oxidative stress in macrophyte was investigated using the multivariate modeling approaches. Cluster analysis rendered two fairly distinct clusters (roots and shoots) of similar characteristics in terms of their biochemical responses. Discriminant analysis identified ascorbate peroxidase (APX) as discriminating variable between the root and shoot tissues. Principal components analysis results suggested that malondialdehyde (MDA), superoxide dismutase (SOD), APX, non-protein thiols (NP-SH), cysteine, ascorbic acid, and Cr-accumulation are dominant in root tissues, whereas, protein and guaiacol peroxidase (GPX) in shoots of the plant. Discriminant partial least squares analysis results further confirmed that MDA, SOD, NP-SH, cysteine, GPX, APX, ascorbic acid and Cr-accumulation dominated in the root tissues, while protein in the shoot. Three-way analysis helped in visualizing simultaneous influence of metal concentration and exposure duration on biochemical variables in plant tissues. The multivariate approaches, thus, allowed for the interpretation of the induced biochemical changes in the plant tissues exposed to chromium, which otherwise using the conventional approaches is difficult.
Sinha
Sarita
S
Ecotoxicology and Bioremediation Group, National Botanical Research Institute (Council of Scientific & Industrial Research), Rana Pratap Marg, Lucknow 226001, India. sinha_sarita52@yahoo.com
Basant
Ankita
A
Malik
Amrita
A
Singh
Kunwar P
KP
D029064:Araceae / Q000187:drug effects* / Q000378:metabolism; D002857:Chromium / Q000378:metabolism / Q000633:toxicity*; D016000:Cluster Analysis; D016002:Discriminant Analysis; D016018:Least-Squares Analysis; D008954:Models, Biological; D015999:Multivariate Analysis; D018384:Oxidative Stress / Q000187:drug effects*; D025341:Principal Component Analysis
D002857:Chromium; C074702:chromium hexavalent ion