title: Glycosylation-related gene expression profiling in the brain and spleen of scrapie-affected mouse.
abstract: A central event in the formation of infectious prions is the conformational change of a host-encoded glycoprotein, PrP(C), into a pathogenic isoform, PrP(Sc). The molecular requirements for efficient PrP conversion remain unknown. Altered glycosylation has been linked to various pathologies and the N-glycans harbored by two prion protein isoforms are different. In order to search for glycosylation-related genes that could mark prion infection, we used a glycosylation-dedicated microarray that allowed the simultaneous analysis of the expression of 165 glycosylation-related genes encoding proteins of the glycosyltransferase, glycosidase, lectin, and sulfotransferase families to compare the gene expression profiles of normal and scrapie-infected mouse brain and spleen. Eight genes were found upregulated in "scrapie brain" at the final state of the disease. In the spleen, five genes presented a modified expression. Three genes were also upregulated in the spleen of infected mice, and two (Pigq and St3gal5) downregulated. All changes were confirmed by qPCR and biochemical analyses applied to Pigq and St3gal5 proteins.
authors.affiliation: INRA, UMR1061 Génétique Moléculaire Animale, Université de Limoges, Limoges, France.
authors.forename: Florence
authors.initials: F
authors.lastname: Guillerme-Bosselut
authors.forename: Lionel
authors.initials: L
authors.lastname: Forestier
authors.forename: Chantal
authors.initials: C
authors.lastname: Jayat-Vignoles
authors.forename: Jean-Luc
authors.initials: JL
authors.lastname: Vilotte
authors.forename: Iuliana
authors.initials: I
authors.lastname: Popa
authors.forename: Jacques
authors.initials: J
authors.lastname: Portoukalian
authors.forename: Annick
authors.initials: A
authors.lastname: Le Dur
authors.forename: Hubert
authors.initials: H
authors.lastname: Laude
authors.forename: Raymond
authors.initials: R
authors.lastname: Julien
authors.forename: Paul-François
authors.initials: PF
authors.lastname: Gallet
mesh_terms: D000818:Animals; D001921:Brain / Q000378:metabolism*; D005260:Female; D020869:Gene Expression Profiling; D006023:Glycoproteins / Q000378:metabolism*; D006031:Glycosylation; D051379:Mice; D046228:Microarray Analysis; D018620:PrPSc Proteins / Q000378:metabolism*; D012608:Scrapie / Q000378:metabolism*; D013154:Spleen / Q000378:metabolism*
chemical_list: D006023:Glycoproteins; D018620:PrPSc Proteins