Differential diagnosis of orf viruses by a single-step PCR.
The complete nucleotide sequence of the A32L gene (named after vaccinia virus, corresponding with open reading frame 108 of the orf virus and encoding an ATPase) of the orf virus was studied using samples of orf virus from infected goats, which were collected from six outbreaks in central Taiwan. DNA sequence analysis of the A32L genes of these and isolates from other countries showed sequence heterogeneity (base pair variation and deletion) in the 3'-terminal regions. This finding led to the development of a polymerase chain reaction (PCR) method for the rapid differential diagnosis of orf virus infections, and the results demonstrated that this was an easy and reliable method for genotyping of orf viruses.
Chan
Kun-Wei
KW
Department of Veterinary Medicine, National Chung-Hsing University, Taichung, Taiwan.
Hsu
Wei-Li
WL
Wang
Chi-Young
CY
Yang
Cheng-Hsiung
CH
Lin
Fong-Yuan
FY
Chulakasian
Songkhla
S
Wong
Min-Liang
ML
D000251:Adenosine Triphosphatases / Q000235:genetics; D000818:Animals; D001483:Base Sequence; D003937:Diagnosis, Differential; D004196:Disease Outbreaks; D004474:Ecthyma, Contagious / Q000175:diagnosis* / Q000453:epidemiology / Q000821:virology*; D005838:Genotype; D006041:Goats; D008969:Molecular Sequence Data; D009923:Orf virus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000379:methods*; D011110:Polymorphism, Genetic; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D013624:Taiwan; D014764:Viral Proteins / Q000235:genetics
D014764:Viral Proteins; D000251:Adenosine Triphosphatases