title: Analysis of loss of heterozygosity in circulating DNA.
abstract: Analysis of genetic altera tion in circulating DNA can have clinical utility in predicting disease outcome. Loss of heterozygosity (LOH) of DNA microsatellites has been shown to occur commonly among all chromosomes in various cancers, such as melanoma, breast cancer, and lung cancer. In this protocol, we focused on the utility of LOH of microsatellite biomarkers for detection of analyzing circulating DNA. The protocol describes how PCR is performed on each patient's paired DNA samples (normal lymphocyte DNA and serum DNA) using specific microsatellite biomarkers followed by post-PCR product analysis using capillary array electrophoresis (CAE). The utility of CAE is due to its digitalization and accuracy of the post-PCR product results.
authors.affiliation: Department of Molecular Oncology, John Wayne Cancer Institute, Saint John's Health Center, Santa Monica, CA, USA.
authors.forename: Takeshi
authors.initials: T
authors.lastname: Nakamura
authors.forename: Eiji
authors.initials: E
authors.lastname: Sunami
authors.forename: Tuny
authors.initials: T
authors.lastname: Nguyen
authors.forename: Dave S B
authors.initials: DS
authors.lastname: Hoon
mesh_terms: D004247:DNA / Q000097:blood*; D019075:Electrophoresis, Capillary; D006801:Humans; D019656:Loss of Heterozygosity / Q000235:genetics*; D008214:Lymphocytes / Q000378:metabolism; D008967:Molecular Biology / Q000379:methods*
chemical_list: D004247:DNA