Proteomic analysis of Caenorhabditis elegans.
Proteomic studies of the free-living nematode Caenorhabditis elegans have recently received great attention because this animal model is a useful platform for the in vivo study of various biological problems relevant to human disease. In general, proteomic analysis is carried out in order to address a specific question with respect to differential changes in proteome expression under certain perturbed conditions. In this chapter, we focus on gel-based proteomic analysis of C. elegans subjected to two specific stress conditions during development: induction of the dauer state for whole body protein expression and a temperature shift for egg protein expression. Utilizing these differently perturbed C. elegans protein samples, two-dimensional electrophoresis and differential in-gel electrophoresis methods have led to the discovery of remarkable aspects of the worm's biology. We also provide numerous details about the technical points and protocols necessary for successful experimentation.
Jeong
Pan-Young
PY
Yonsei Proteome Research Center, Biomedical Proteome Research Center and Department of Biochemistry, Yonsei University, 134 Shinchon-dong, Sudamoon-Ku, Seoul, 120-749, Korea.
Na
Keun
K
Jeong
Mi-Jeong
MJ
Chitwood
David
D
Shim
Yhong-Hee
YH
Paik
Young-Ki
YK
D000818:Animals; D017173:Caenorhabditis elegans / Q000737:chemistry* / Q000502:physiology; D029742:Caenorhabditis elegans Proteins / Q000032:analysis*; D004527:Egg Proteins / Q000032:analysis; D015180:Electrophoresis, Gel, Two-Dimensional / Q000295:instrumentation / Q000379:methods; D006801:Humans; D007525:Isoelectric Focusing / Q000295:instrumentation / Q000379:methods; D008969:Molecular Sequence Data; D020543:Proteome / Q000032:analysis*; D013696:Temperature
D029742:Caenorhabditis elegans Proteins; D004527:Egg Proteins; D020543:Proteome