title: Multiple integrated copies and high-level production of the human retrovirus XMRV (xenotropic murine leukemia virus-related virus) from 22Rv1 prostate carcinoma cells.
abstract: The human retrovirus XMRV (xenotropic murine leukemia virus-related virus) is associated with prostate cancer, most frequently in humans with a defect in the antiviral defense protein RNase L, suggesting a role for XMRV in prostate carcinogenesis. However, XMRV has not been found in prostate carcinoma cells. Here we show that 22Rv1 prostate carcinoma cells produce high-titer virus that is nearly identical in properties and sequence to XMRV isolated by others and consist primarily of a single clone of cells with at least 10 integrated copies of XMRV, warranting further study of a possible role for XMRV integration in carcinogenesis.
authors.affiliation: Fred Hutchinson Cancer Research Center, Seattle, Washington 98109-1024, USA.
authors.forename: Emily C
authors.initials: EC
authors.lastname: Knouf
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Metzger
authors.forename: Patrick S
authors.initials: PS
authors.lastname: Mitchell
authors.forename: Jason D
authors.initials: JD
authors.lastname: Arroyo
authors.forename: John R
authors.initials: JR
authors.lastname: Chevillet
authors.forename: Muneesh
authors.initials: M
authors.lastname: Tewari
authors.forename: A Dusty
authors.initials: AD
authors.lastname: Miller
mesh_terms: D000818:Animals; D002277:Carcinoma / Q000821:virology*; D045744:Cell Line, Tumor; D018628:Gene Dosage; D006801:Humans; D008297:Male; D051379:Mice; D010802:Phylogeny; D011471:Prostatic Neoplasms / Q000821:virology*; D012190:Retroviridae / Q000145:classification / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology*; D016662:Virus Integration