Scanning ion conductance microscopy: a model for experimentally realistic conditions and image interpretation.
Scanning ion conductance microscopy (SICM) is a scanned probe microscopy technique in which the probe is a fine glass pipet filled with a contact (reference) electrode and an electrolyte solution. The current flow between the reference electrode and a second reference electrode positioned in bulk solution when the two electrodes are biased externally can be used as a feedback signal to maintain a constant separation between the tip and a surface during imaging. In usual practice the tip position is modulated over a small amplitude perpendicular to the surface, and the resulting alternating current (AC) is used as the feedback signal, although the direct current can also be used. A comprehensive model for the current response is reported. Laplace's equation has been solved for the electrolyte solution for a range of tip geometries, enabling the factors controlling the tip current to be identified. The approach developed is shown to represent an improvement over earlier semiempirical treatments. To explore the influence of surface topography on the (AC) current response, various surfaces have been considered, including a tip moved toward a planar surface (in the normal direction) and tips scanned over a pit and a step in the surface. The results have allowed a critical assessment of the SICM response as a means of probing surface topography. Features identified through simulation have been found in experiments through studies of two model substrates for which imaging results are reported. In typical experimental practice, the response of the SICM tip to surface features occurs over much greater lateral distances than the size of the pipet aperture.
Edwards
Martin A
MA
Department of Chemistry, University of Warwick, Coventry CV4 7AL, UK.
Williams
Cara G
CG
Whitworth
Anna L
AL
Unwin
Patrick R
PR
D003198:Computer Simulation; D004553:Electric Conductivity; D004563:Electrochemistry / Q000295:instrumentation / Q000379:methods*; D004566:Electrodes; D004573:Electrolytes / Q000737:chemistry; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D020527:Microscopy, Scanning Probe / Q000295:instrumentation* / Q000379:methods*; D008956:Models, Chemical; D013499:Surface Properties
D004573:Electrolytes