title: Genetic polymorphisms of FSHR, CYP17, CYP1A1, CAPN10, INSR, SERPINE1 genes in adolescent girls with polycystic ovary syndrome.
abstract: BACKGROUND
  Polycystic ovary syndrome (PCOS), whose genetic basis is not completely well understood, is the most common endocrine disorder in women and it typically develops during adolescence. The aim of this study is to investigate the possible association between single nucleotide polymorphisms (SNPs) of FSHR, CYP17, CYP1A1, CAPN10, INSR, SERPINE1 genes and PCOS in adolescent girls.
  
  
  METHODS
  DNA samples from forty-four adolescent girls with PCOS and 50 healthy controls were analyzed by PCR-RFLP and direct DNA sequencing to determine the genotypic frequency of 17 different polymorphic loci on the FSHR (A307T, N680S), CYP17 (-34 T/C), CYP1A1 (T6235C), CAPN10 (44, 43, 19, 63), INSR (exon 17 C/T), SERPINE1 (4G/5G) genes. Genotyping of exon 12 (six polymorphisms) and intron 12 (one polymorphism) of INSR gene by direct DNA sequencing was performed for the first time in this study.
  
  
  RESULTS
  No significant differences were observed in the genotype and allele distributions of above mentioned polymorphisms between cases and control groups.
  
  
  CONCLUSIONS
  Our data does not support an association between SNPs of FSHR, CYP17, CYP1A1, CAPN10, INSR, SERPINE1 genes and susceptibility to PCOS or related traits in Turkish adolescent girls.
authors.affiliation: Department of Medical Biology and Genetics, Faculty of Medicine, Gazi University, Besevler, Ankara, Turkey.
authors.forename: Tugba
authors.initials: T
authors.lastname: Unsal
authors.forename: Ece
authors.initials: E
authors.lastname: Konac
authors.forename: Ediz
authors.initials: E
authors.lastname: Yesilkaya
authors.forename: Akin
authors.initials: A
authors.lastname: Yilmaz
authors.forename: Aysun
authors.initials: A
authors.lastname: Bideci
authors.forename: Hacer
authors.initials: H
authors.lastname: Ilke Onen
authors.forename: Peyami
authors.initials: P
authors.lastname: Cinaz
authors.forename: Adnan
authors.initials: A
authors.lastname: Menevse
mesh_terms: D000293:Adolescent; D015703:Antigens, CD / Q000235:genetics; D002154:Calpain / Q000235:genetics; D019363:Cytochrome P-450 CYP1A1 / Q000235:genetics; D005260:Female; D020022:Genetic Predisposition to Disease; D006801:Humans; D017395:Plasminogen Activator Inhibitor 1 / Q000235:genetics; D011085:Polycystic Ovary Syndrome / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide; D011972:Receptor, Insulin / Q000235:genetics; D011962:Receptors, FSH / Q000235:genetics; D013254:Steroid 17-alpha-Hydroxylase / Q000235:genetics
chemical_list: D015703:Antigens, CD; D017395:Plasminogen Activator Inhibitor 1; D011962:Receptors, FSH; C051321:SERPINE1 protein, human; D019363:Cytochrome P-450 CYP1A1; D013254:Steroid 17-alpha-Hydroxylase; C435941:INSR protein, human; D011972:Receptor, Insulin; D002154:Calpain; C415197:calpain 10