Multiple integrated copies and high-level production of the human retrovirus XMRV (xenotropic murine leukemia virus-related virus) from 22Rv1 prostate carcinoma cells.
The human retrovirus XMRV (xenotropic murine leukemia virus-related virus) is associated with prostate cancer, most frequently in humans with a defect in the antiviral defense protein RNase L, suggesting a role for XMRV in prostate carcinogenesis. However, XMRV has not been found in prostate carcinoma cells. Here we show that 22Rv1 prostate carcinoma cells produce high-titer virus that is nearly identical in properties and sequence to XMRV isolated by others and consist primarily of a single clone of cells with at least 10 integrated copies of XMRV, warranting further study of a possible role for XMRV integration in carcinogenesis.
Knouf
Emily C
EC
Fred Hutchinson Cancer Research Center, Seattle, Washington 98109-1024, USA.
Metzger
Michael J
MJ
Mitchell
Patrick S
PS
Arroyo
Jason D
JD
Chevillet
John R
JR
Tewari
Muneesh
M
Miller
A Dusty
AD
D000818:Animals; D002277:Carcinoma / Q000821:virology*; D045744:Cell Line, Tumor; D018628:Gene Dosage; D006801:Humans; D008297:Male; D051379:Mice; D010802:Phylogeny; D011471:Prostatic Neoplasms / Q000821:virology*; D012190:Retroviridae / Q000145:classification / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology*; D016662:Virus Integration