Autofluorescence of the fruiting body of the fungus Macrolepiota rhacodes.
The autofluorescence (primary fluorescence, AF) of the freshly collected fruiting bodies of the fungus Macrolepiota rhacodes was studied in a Zeiss Jenalumar fluorescence microscope at a blue and a green excitation. The strongest yellow AF at blue excitation was displayed by irregular granules on the surface of the fungal pileus. A weaker yellow-green AF was exhibited by spherical cells and hyphae in the central part of the pileus while basidiospores emitted somewhat stronger AF. At green excitation, a considerable red AF was emitted only by basidiospores, other parts of the pileus showing a very weak red AF. M. rhacodes AF is much weaker than the AF of wood-rotting fungi, such as Fomes fomentarius, Daedalea quercina, Piptoporus betulinus, Fomitopsis pinicola and others.
Zizka
Z
Z
Institute of Microbiology, Academy of Sciences of the Czech Repulic, Prague, Czechia. zizka@biomed.cas.cz
Gabriel
J
J
D000363:Agaricales / Q000166:cytology / Q000254:growth & development / Q000502:physiology*; D005453:Fluorescence; D048690:Fruiting Bodies, Fungal / Q000166:cytology / Q000502:physiology*; D025301:Hyphae / Q000502:physiology / Q000648:ultrastructure; D008856:Microscopy, Fluorescence; D013045:Species Specificity; D013172:Spores, Fungal / Q000502:physiology