Two anion transporters AtClCa and AtClCe fulfil interconnecting but not redundant roles in nitrate assimilation pathways.
* In plants, the knowledge of the molecular identity and functions of anion channels are still very limited, and are almost restricted to the large ChLoride Channel (CLC) family. In Arabidopsis thaliana, some genetic evidence has suggested a role for certain AtCLC protein members in the control of plant nitrate levels. In this context, AtClCa has been demonstrated to be involved in nitrate transport into the vacuole, thereby participating in cell nitrate homeostasis. * In this study, analyses of T-DNA insertion mutants within the AtClCa and AtClCe genes revealed common phenotypic traits: a lower endogenous nitrate content; a higher nitrite content; a reduced nitrate influx into the root; and a decreased expression of several genes encoding nitrate transporters. * This set of nitrate-related phenotypes, displayed by clca and clce mutant plants, showed interconnecting roles of AtClCa and AtClCe in nitrate homeostasis involving two different endocellular membranes. * In addition, it revealed cross-talk between two nitrate transporter families participating in nitrate assimilation pathways. The contribution to nitrate homeostasis at the cellular level of members of these different families is discussed.
Monachello
Dario
D
Institut des Sciences du Végétal, Centre National de la Recherche Scientifique (CNRS - UPR 2355), Bât 22, avenue de la Terrasse, 91198 Gif sur Yvette Cedex, France.
Allot
Michèle
M
Institut des Sciences du Végétal, Centre National de la Recherche Scientifique (CNRS - UPR 2355), Bât 22, avenue de la Terrasse, 91198 Gif sur Yvette Cedex, France.
Oliva
Sabrina
S
Unité de Nutrition Azotée des Plantes, Institut National de la Recherche Agronomique, Route de Saint-Cyr, 78026 Versailles Cedex, France.
Krapp
Anne
A
Unité de Nutrition Azotée des Plantes, Institut National de la Recherche Agronomique, Route de Saint-Cyr, 78026 Versailles Cedex, France.
Daniel-Vedele
Françoise
F
Unité de Nutrition Azotée des Plantes, Institut National de la Recherche Agronomique, Route de Saint-Cyr, 78026 Versailles Cedex, France.
Barbier-Brygoo
Hélène
H
Institut des Sciences du Végétal, Centre National de la Recherche Scientifique (CNRS - UPR 2355), Bât 22, avenue de la Terrasse, 91198 Gif sur Yvette Cedex, France.
Ephritikhine
Geneviève
G
Institut des Sciences du Végétal, Centre National de la Recherche Scientifique (CNRS - UPR 2355), Bât 22, avenue de la Terrasse, 91198 Gif sur Yvette Cedex, France.|Université Paris 7-Denis Diderot, UFR Sciences du Vivant, 2 place Jussieu, 75251 Paris Cedex 05, France.
D027321:Anion Transport Proteins / Q000235:genetics / Q000378:metabolism*; D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D018118:Chloride Channels / Q000235:genetics / Q000378:metabolism*; D004269:DNA, Bacterial; D017343:Genes, Plant; D007425:Intracellular Membranes / Q000378:metabolism; D017136:Ion Transport / Q000502:physiology*; D053858:Metabolic Networks and Pathways; D009154:Mutation; D000091285:Nitrate Transporters; D009566:Nitrates / Q000378:metabolism*; D009573:Nitrites / Q000378:metabolism*; D010641:Phenotype; D020239:Receptor Cross-Talk; D015398:Signal Transduction; D014617:Vacuoles / Q000378:metabolism
C407524:ATCLC-A protein, Arabidopsis; D027321:Anion Transport Proteins; D029681:Arabidopsis Proteins; D018118:Chloride Channels; C545081:ClCe protein, Arabidopsis; D004269:DNA, Bacterial; D000091285:Nitrate Transporters; D009566:Nitrates; D009573:Nitrites; C036483:T-DNA