AFM and multiple transmission-reflection infrared spectroscopy (MTR-IR) studies on formation of air-stable supported lipid bilayers.
Supported lipid bilayers (SLBs) were prepared by deposition of unilamellar vesicles on a silicon substrate. Atomic force microscopy (AFM) and a new Multiple Transmission-Reflection Infrared Spectroscopy (MTR-IR) developed by us were used to trace the dynamic formation of lipid bilayers on the silicon surfaces. The evolution from deformation of vesicles to formation of bilayers can be distinguished clearly by AFM imaging. MTR-IR provided high quality infrared spectra of ultrathin lipid bilayers with high sensitivity and high signal to noise ratio (SNR). The structural and orientational changes during vesicle's fusion were monitored with MTR-IR. MTR-IR shows superiority over other infrared approaches for ultrathin films on standard silicon wafers in view of its economy and high sensitivity. Both MTR-IR and AFM results were consistent with each other and they provided more information for understanding the self-assembling procedure of SLBs.
Guo
Peng-Feng
PF
State Key Laboratory of Coordination Chemistry, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093, P.R. China.
Huang
Wen-Yi
WY
State Key Laboratory of Coordination Chemistry, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093, P.R. China.
Liu
Hong-Bo
HB
State Key Laboratory of Coordination Chemistry, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093, P.R. China.
Xiao
Shou-Jun
SJ
State Key Laboratory of Coordination Chemistry, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093, P.R. China.
D000818:Animals; D002645:Chickens; D004530:Egg Yolk / Q000737:chemistry; D008051:Lipid Bilayers / Q000737:chemistry*; D018625:Microscopy, Atomic Force; D010713:Phosphatidylcholines / Q000737:chemistry; D059629:Signal-To-Noise Ratio; D012825:Silicon / Q000737:chemistry; D013055:Spectrophotometry, Infrared; D053835:Unilamellar Liposomes / Q000737:chemistry
D008051:Lipid Bilayers; D010713:Phosphatidylcholines; D053835:Unilamellar Liposomes; D012825:Silicon