Crystallization and preliminary X-ray diffraction analysis of a protease-resistant mutant form of human galectin-8.
A crystal of a protease-resistant mutant form of human galectin-8, a tandem-repeat-type galectin with two carbohydrate-recognition domains, was obtained using the hanging-drop method and was found to belong to the tetragonal space group P4(3)2(1)2, with unit-cell parameters a = 78.93, b = 78.93, c = 132.05 A. Diffraction data were collected to a resolution of 3.4 A.
Yoshida
Hiromi
H
Life Science Research Center, Kagawa University, Ikenobe, Miki-cho, Kita-gun, Japan.
Nishi
Nozomu
N
Nakakita
Shin-ichi
S
Kamitori
Shigehiro
S
D003460:Crystallization; D018360:Crystallography, X-Ray; D037161:Galectins / Q000032:analysis* / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D006801:Humans; D009154:Mutation / Q000235:genetics; D010447:Peptide Hydrolases / Q000378:metabolism*
D037161:Galectins; C091991:LGALS8 protein, human; D010447:Peptide Hydrolases