title: Cytogenetic biomonitoring of Brazilian workers exposed to pesticides: micronucleus analysis in buccal epithelial cells of soybean growers.
abstract: Pesticides have been considered potential chemical mutagens and various agrochemical ingredients possess mutagenic properties. Biomonitoring provides a useful tool to estimate the genetic risk from exposure to a complex mixture of chemicals. In general genetic damage associated with pesticides occurs in human populations subject to high exposure levels due to intensive use, misuse or failure of control measures. Few studies have been carried out using the micronucleus (MN) analysis in buccal cells of farm workers and, from the available data, only one has found a positive relationship. Micronuclei were analyzed in 29 Brazilian workers exposed to pesticides in soybean fields and in 37 non-exposed individuals. The results obtained indicate that the mean number of cells with MN in the exposed group (3.55+/-2.13) was significantly higher than in the control group (1.78+/-1.23). The number of cells with MN was not influenced by age, smoking habit, smoking time, number of cigarettes/day, alcohol consumption and years of exposure to pesticides. The genotoxic potential of the pesticides used in soybean fields may explain the detectable increase of cells with MN in exposed workers.
authors.affiliation: Curso de Biomedicina, Instituto de Ciências da Saúde, Centro Universitário Feevale, Brazil.
authors.forename: Giorgia Moura de
authors.initials: GM
authors.lastname: Bortoli
authors.forename: Mariana Barbieri de
authors.initials: MB
authors.lastname: Azevedo
authors.forename: Luciano Basso da
authors.initials: LB
authors.lastname: Silva
mesh_terms: D000293:Adolescent; D000328:Adult; D000382:Agricultural Workers' Diseases / Q000175:diagnosis / Q000209:etiology* / Q000235:genetics; D001938:Brazil; D006801:Humans; D048629:Micronuclei, Chromosome-Defective / Q000139:chemically induced; D015162:Micronucleus Tests; D008875:Middle Aged; D009061:Mouth Mucosa / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D016273:Occupational Exposure / Q000009:adverse effects / Q000032:analysis*; D010575:Pesticides / Q000506:poisoning*; D012307:Risk Factors; D013025:Glycine max / Q000254:growth & development; D055815:Young Adult
chemical_list: D010575:Pesticides