title: Metalloproteinase-dependent shedding of low-density lipoprotein receptor-related protein-1 ectodomain decreases endocytic clearance of endometrial matrix metalloproteinase-2 and -9 at menstruation.
abstract: Cyclic elimination of the endometrium functional layer through menstrual bleeding results from intense tissue breakdown by proteolytic enzymes, mainly members of the matrix metalloproteinase (MMP) family. In contrast to menstrual-restricted MMPs, e.g. interstitial collagenase (MMP-1), gelatinases A (MMP-2) and B (MMP-9) mRNAs are abundant throughout the cycle without detectable tissue degradation at proliferative and secretory phases, implying a tight posttranslational control of both gelatinases. This paper addresses the role of low-density lipoprotein receptor-related protein (LRP)-1 in the endocytic clearance of endometrial gelatinases. LRP-1 mRNA and protein were studied using RT-PCR, Western blotting, and immunolabeling. Posttranslational control of LRP-1 was analyzed in explant culture. The receptor-associated protein (RAP), used as LRP antagonist, strongly increased (pro)gelatinase accumulation in medium conditioned by endometrial explants, suggesting a role for LRP-1 in their clearance. Although LRP-1 mRNA remained constant throughout the cycle, the protein ectodomain vanished at menses. LRP-1 immunolabeling selectively disappeared in areas of extracellular matrix breakdown in menstrual samples. It also disappeared from explants cultured without estrogen and progesterone (EP) due to ectodomain shedding in the medium. The shedding was inhibited by metalloproteinase inhibitors, including a disintegrin and metalloproteinase (ADAM) inhibitor, and by tissue inhibitors of MMPs (TIMP)-3 and -2, but barely by TIMP-1, pointing to ADAM-12 as the putative sheddase. In good agreement, ADAM-12 mRNA expression was repressed by EP. In conclusion, the efficient LRP-1-mediated clearance of gelatinase activity in nonbleeding endometrium is abrogated upon EP withdrawal, due to shedding of LRP-1 ectodomain by a metalloproteinase, presumably ADAM-12, itself regulated by EP.
authors.affiliation: Cell Biology Unit, de Duve Institute, Université catholique de Louvain, B-1200 Brussels, Belgium.
authors.forename: Charlotte
authors.initials: C
authors.lastname: Selvais
authors.forename: Héloïse P
authors.initials: HP
authors.lastname: Gaide Chevronnay
authors.forename: Pascale
authors.initials: P
authors.lastname: Lemoine
authors.forename: Stéphane
authors.initials: S
authors.lastname: Dedieu
authors.forename: Patrick
authors.initials: P
authors.lastname: Henriet
authors.forename: Pierre J
authors.initials: PJ
authors.lastname: Courtoy
authors.forename: Etienne
authors.initials: E
authors.lastname: Marbaix
authors.forename: Hervé
authors.initials: H
authors.lastname: Emonard
mesh_terms: D015153:Blotting, Western; D004717:Endometrium / Q000187:drug effects / Q000378:metabolism*; D005260:Female; D018093:Gelatinases / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D066298:In Vitro Techniques; D026503:Low Density Lipoprotein Receptor-Related Protein-1 / Q000378:metabolism*; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism*; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism*; D008598:Menstruation / Q000187:drug effects / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D011374:Progesterone / Q000494:pharmacology; D011372:Progestins / Q000494:pharmacology; D019716:Tissue Inhibitor of Metalloproteinase-2 / Q000494:pharmacology; D019717:Tissue Inhibitor of Metalloproteinase-3 / Q000494:pharmacology
chemical_list: D026503:Low Density Lipoprotein Receptor-Related Protein-1; D011372:Progestins; D019717:Tissue Inhibitor of Metalloproteinase-3; D019716:Tissue Inhibitor of Metalloproteinase-2; D011374:Progesterone; D018093:Gelatinases; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9