Benzaldehyde dehydrogenase from chitosan-treated Sorbus aucuparia cell cultures.
Cell cultures of Sorbus aucuparia respond to the addition of chitosan with the accumulation of the biphenyl phytoalexin aucuparin. The carbon skeleton of this inducible defense compound is formed by biphenyl synthase (BIS) from benzoyl-CoA and three molecules of malonyl-CoA. The formation of benzoyl-CoA proceeds via benzaldehyde as an intermediate. Benzaldehyde dehydrogenase (BD), which converts benzaldehyde into benzoic acid, was detected in cell-free extracts from S. aucuparia cell cultures. BD and BIS were induced by chitosan treatment. The preferred substrate for BD was benzaldehyde (K(m)=49 microM). Cinnamaldehyde and various hydroxybenzaldehydes were relatively poor substrates. BD activity was strictly dependent on the presence of NAD(+) as a cofactor (K(m)=67 microM).
Gaid
Mariam M
MM
Institute of Pharmaceutical Biology, Technical University of Braunschweig, Mendelssohnstrasse 1, D-38106 Braunschweig, Germany.
Sircar
Debabrata
D
Beuerle
Till
T
Mitra
Adinpunya
A
Beerhues
Ludger
L
D000445:Aldehyde Oxidoreductases / Q000378:metabolism*; D001547:Benzaldehydes / Q000737:chemistry; D055162:Biocatalysis / Q000187:drug effects; D002478:Cells, Cultured; D048271:Chitosan / Q000494:pharmacology*; D002851:Chromatography, High Pressure Liquid; D008401:Gas Chromatography-Mass Spectrometry; D007700:Kinetics; D009097:Multienzyme Complexes / Q000378:metabolism; D031994:Sorbus / Q000166:cytology / Q000187:drug effects* / Q000201:enzymology*; D013379:Substrate Specificity / Q000187:drug effects
D001547:Benzaldehydes; D009097:Multienzyme Complexes; C482658:biphenyl synthase, Sorbus aucuparia; D048271:Chitosan; D000445:Aldehyde Oxidoreductases; C027015:benzaldehyde dehydrogenase (NAD+); C032175:benzaldehyde