Cellular mRNA expressions of anti-oxidant factors in the blood of preeclamptic women.
OBJECTIVE
To assess the alterations of mRNA expressions associated with oxidative stress in the cellular component of blood from pregnant women with pre-eclampsia.


METHODS
Peripheral blood samples were obtained from pregnant women with and without pre-eclampsia. Cellular RNA was subjected to a reverse transcription polymerase chain reaction (PCR) assay in order to examine the mRNA distribution among women with pre-eclampsia (n = 24) and control subjects (n = 24) during 35-41 weeks of gestation. The data were analyzed by non-parametric statistics.


RESULTS
Significant differences between the pre-eclampsia subjects and the controls were observed in the gene expressions associated with oxidative stress. Lower values in the pre-eclampsia group were found for heme oxygenase (HO)-1, HO-2, catalase and superoxide dismutase (SOD). The HO-1, HO-2 and the catalase levels significantly correlated with proteinuria, and the HO-2 level with systolic blood pressure.


CONCLUSIONS
Significantly lower concentrations of HO-1, HO-2, SOD and catalase are found in the cellular component of blood from pre-eclamptic patients. The values correlate with the severity of pre-eclampsia. These findings indicate that enhanced oxidative stress and a decrease in the number of anti-oxidant enzymes may be associated with pre-eclampsia.
Nakamura
Masamitsu
M
Department of Obstetrics and Gynecology, Showa University School of Medicine, Tokyo, Japan.
Sekizawa
Akihiko
A
Purwosunu
Yuditiya
Y
Okazaki
Shiho
S
Farina
Antonio
A
Wibowo
Noroyono
N
Shimizu
Hanako
H
Okai
Takashi
T
D000293:Adolescent; D000328:Adult; D000975:Antioxidants / Q000378:metabolism*; D001773:Blood Cells / Q000378:metabolism*; D001794:Blood Pressure; D002374:Catalase / Q000235:genetics / Q000378:metabolism; D005260:Female; D017359:HELLP Syndrome / Q000097:blood / Q000235:genetics / Q000378:metabolism / Q000503:physiopathology; D006419:Heme Oxygenase (Decyclizing) / Q000235:genetics / Q000378:metabolism; D051547:Heme Oxygenase-1 / Q000235:genetics / Q000378:metabolism; D006801:Humans; D018384:Oxidative Stress / Q000235:genetics / Q000502:physiology; D011225:Pre-Eclampsia / Q000097:blood* / Q000235:genetics* / Q000378:metabolism / Q000503:physiopathology; D011247:Pregnancy; D012333:RNA, Messenger / Q000097:blood* / Q000378:metabolism; D013482:Superoxide Dismutase / Q000235:genetics / Q000378:metabolism; D055815:Young Adult
D000975:Antioxidants; D012333:RNA, Messenger; D002374:Catalase; D006419:Heme Oxygenase (Decyclizing); D051547:Heme Oxygenase-1; C105245:heme oxygenase-2; D013482:Superoxide Dismutase