title: Generate Western blot protein marker from a single construct.
abstract: An expression construct, consisting of a tandem arrangement of nucleic acids coding for the constant fragments (Fc) receptor of protein G combined with nucleic acids for the Fc receptor of protein A, was constructed. When the construct was expressed in Escherichia coli, proteins of estimated molecular weights of 25, 30, 50, 58, 80, and 85 kDa were consistently obtained from this expression construct due to possible proteolytic degradation during the cultivation and purification steps. The purified proteins from this single expression construct were used as Western blot protein marker.
authors.affiliation: Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China.
authors.forename: Yi-Ming
authors.initials: YM
authors.lastname: Zhang
authors.forename: Liang
authors.initials: L
authors.lastname: Geng
authors.forename: Da-Wei
authors.initials: DW
authors.lastname: Huang
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000032:analysis* / Q000235:genetics / Q000302:isolation & purification; D015153:Blotting, Western / Q000379:methods*; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics; D008969:Molecular Sequence Data; D008970:Molecular Weight; D011961:Receptors, Fc / Q000032:analysis* / Q000235:genetics / Q000302:isolation & purification; D013205:Staphylococcal Protein A / Q000032:analysis* / Q000235:genetics / Q000302:isolation & purification
chemical_list: D001426:Bacterial Proteins; C034284:IgG Fc-binding protein, Streptococcus; D011961:Receptors, Fc; D013205:Staphylococcal Protein A