Biosensing by optical waveguide spectroscopy based on localized surface plasmon resonance of gold nanoparticles used as a probe or as a label.
The application of localized surface plasmon resonance (LSPR) of gold nanoparticles for the detection of biotin-streptavidin binding, as a typical biological reaction, was investigated by using optical waveguide spectroscopy, and two different modes for the use of gold nanoparticles, one as a probe and the other as a label were compared with each other. The combination with optical waveguide spectroscopy was found to bring about a high sensitivity for the biomolecular detection system using LSPR of gold nanoparticles in both modes. In particular, the mode using gold nanoparticles as a label was demonstrated to be of advantage to devising proper procedures for using nanoparticles and evaluating actual response relevant to the phenomenon concerned, and thus to sensitive detection.
Kajiura
Mayuki
M
Consolidated Research Institute for Advanced Science and Medical Care, Waseda University, 3-4-1 Okubo, Shinjuku-ku, Tokyo 169-8555, Japan.
Nakanishi
Takuya
T
Iida
Hironori
H
Takada
Harumi
H
Osaka
Tetsuya
T
D015374:Biosensing Techniques; D001710:Biotin / Q000378:metabolism; D006046:Gold / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry*; D011485:Protein Binding; D012680:Sensitivity and Specificity; D019809:Streptavidin / Q000032:analysis* / Q000378:metabolism; D020349:Surface Plasmon Resonance / Q000295:instrumentation / Q000379:methods*
D001710:Biotin; D006046:Gold; D019809:Streptavidin