title: Mesenchymal stem cell-derived microvesicles protect against acute tubular injury.
abstract: Administration of mesenchymal stem cells (MSCs) improves the recovery from acute kidney injury (AKI). The mechanism may involve paracrine factors promoting proliferation of surviving intrinsic epithelial cells, but these factors remain unknown. In the current study, we found that microvesicles derived from human bone marrow MSCs stimulated proliferation in vitro and conferred resistance of tubular epithelial cells to apoptosis. The biologic action of microvesicles required their CD44- and beta1-integrin-dependent incorporation into tubular cells. In vivo, microvesicles accelerated the morphologic and functional recovery of glycerol-induced AKI in SCID mice by inducing proliferation of tubular cells. The effect of microvesicles on the recovery of AKI was similar to the effect of human MSCs. RNase abolished the aforementioned effects of microvesicles in vitro and in vivo, suggesting RNA-dependent biologic effects. Microarray analysis and quantitative real time PCR of microvesicle-RNA extracts indicate that microvesicles shuttle a specific subset of cellular mRNA, such as mRNAs associated with the mesenchymal phenotype and with control of transcription, proliferation, and immunoregulation. These results suggest that microvesicles derived from MSCs may activate a proliferative program in surviving tubular cells after injury via a horizontal transfer of mRNA.
authors.affiliation: Department of Internal Medicine, Research Center for Experimental Medicine, University of Torino, Torino, Italy.
authors.forename: Stefania
authors.initials: S
authors.lastname: Bruno
authors.forename: Cristina
authors.initials: C
authors.lastname: Grange
authors.forename: Maria Chiara
authors.initials: MC
authors.lastname: Deregibus
authors.forename: Raffaele A
authors.initials: RA
authors.lastname: Calogero
authors.forename: Silvia
authors.initials: S
authors.lastname: Saviozzi
authors.forename: Federica
authors.initials: F
authors.lastname: Collino
authors.forename: Laura
authors.initials: L
authors.lastname: Morando
authors.forename: Alessandro
authors.initials: A
authors.lastname: Busca
authors.forename: Michele
authors.initials: M
authors.lastname: Falda
authors.forename: Benedetta
authors.initials: B
authors.lastname: Bussolati
authors.forename: Ciro
authors.initials: C
authors.lastname: Tetta
authors.forename: Giovanni
authors.initials: G
authors.lastname: Camussi
mesh_terms: D000818:Animals; D002455:Cell Division; D017690:Cell Transplantation / Q000379:methods*; D005434:Flow Cytometry; D017930:Genes, Reporter; D005990:Glycerol / Q000633:toxicity; D007674:Kidney Diseases / Q000276:immunology / Q000473:pathology / Q000601:surgery*; D007684:Kidney Tubules / Q000187:drug effects / Q000293:injuries* / Q000473:pathology; D045164:Mesenchymal Stem Cell Transplantation / Q000379:methods*; D059630:Mesenchymal Stem Cells / Q000502:physiology*; D051379:Mice; D016513:Mice, SCID; D020411:Oligonucleotide Array Sequence Analysis; D012333:RNA, Messenger / Q000235:genetics
chemical_list: D012333:RNA, Messenger; D005990:Glycerol