Comparison of adult neurospheres derived from different origins for treatment of rat spinal cord injury.
This study is designed to evaluate the therapeutic effects of three types of neurospheres (NSs) derived from brain, bone marrow and adipose tissue in a rat model of spinal contusive injury. As shown by BBB locomotor rating scale and grid test, the optimal therapeutic responses generated by subventricular zone-derived NSs (SVZ-NSs), and followed by adipose-derived (AD-NSs) and bone marrow-derived NSs (BM-NSs) after being grafted into the injured spinal cord. In three cell-treated groups, very few (<1%) grafted cells survived and these survived cells mainly differentiated into oligodendrocytes at week 12 after injury. Additionally, all the cell-treated groups, especially in the SVZ-treated group showed an increase in host oligodendrocytes than control group. Moreover, the level of selective neurotrophins (NTs) in the SVZ-NSs group were significantly higher than those in the BM-NSs and AD-NSs groups, and the level of NTs in the saline group was also significantly higher than sham group. Therefore, not cell replacement or infusion but neuroprotective action associated with endogenous oligodendrocytes and NTs that active by the grafted NSs may contribute to the functional recovery.
Zhang
Hong-tian
HT
Department of Neurosurgery, Zhujiang Hospital, Southern Medical University, Guangzhou, China.
Cheng
Hao-yu
HY
Cai
Ying-qian
YQ
Ma
Xu
X
Liu
Wen-peng
WP
Yan
Zhong-jie
ZJ
Jiang
Xiao-dan
XD
Xu
Ru-xiang
RX
D000273:Adipose Tissue / Q000637:transplantation; D000818:Animals; D016026:Bone Marrow Transplantation; D008297:Male; D009414:Nerve Growth Factors / Q000378:metabolism; D009416:Nerve Regeneration; D009474:Neurons / Q000637:transplantation*; D009836:Oligodendroglia / Q000502:physiology; D051381:Rats; D017207:Rats, Sprague-Dawley; D020127:Recovery of Function; D013119:Spinal Cord Injuries / Q000503:physiopathology / Q000601:surgery*; D033581:Stem Cell Transplantation
D009414:Nerve Growth Factors