Simple fabrication of a smart microarray of polystyrene microbeads for immunoassay.
We describe a simple method to fabricate an array of polystyrene microbeads (PS microbeads) conjugated with an elastin-like polypeptide (ELP) on a glass surface using a removable polymer template (RPT). A thin layer of adhesive was spun-cast on glass and cured by UV radiation. Micropatterns of an RPT were then transferred onto the surface by microcontact printing. The adhesion of PS microbeads on the surface depended on the adhesion performance of the adhesive layer, which could be adjusted by irradiation time. An array of PS microbeads conjugated with ELP was used for a smart immunoassay of prostate-specific antigen (PSA), a cancer marker. By controlling the phase transition of ELP molecules, PSA molecules were selectively adhered or released from the bead surface. The selective and reversible binding of PSA molecules on the bead surface was characterized with fluorescence microscopy.
Lee
Jonghwan
J
Department of Biosystems and Biomaterials Science and Engineering, Seoul National University, Seoul 151-742, Republic of Korea.
Kim
Okgene
O
Jung
Jaeyeon
J
Na
Kyunga
K
Heo
Pilwoo
P
Hyun
Jinho
J
D007118:Immunoassay / Q000379:methods*; D008863:Microspheres; D010455:Peptides / Q000737:chemistry; D011137:Polystyrenes / Q000737:chemistry*; D017430:Prostate-Specific Antigen / Q000737:chemistry
D010455:Peptides; D011137:Polystyrenes; D017430:Prostate-Specific Antigen