title: Functional analysis of the stress-inducible soybean calmodulin isoform-4 (GmCaM-4) promoter in transgenic tobacco plants.
abstract: The transcription of soybean (Glycine max) calmodulin isoform-4 (GmCaM-4) is dramatically induced within 0.5 h of exposure to pathogen or NaCl. Core cis-acting elements that regulate the expression of the GmCaM-4 gene in response to pathogen and salt stress were previously identified, between -1,207 and -1,128 bp, and between -858 and -728 bp, in the GmCaM-4 promoter. Here, we characterized the properties of the DNA-binding complexes that form at the two core cis-acting elements of the GmCaM-4 promoter in pathogen-treated nuclear extracts. We generated GUS reporter constructs harboring various deletions of approximately 1.3-kb GmCaM-4 promoter, and analyzed GUS expression in tobacco plants transformed with these constructs. The GUS expression analysis suggested that the two previously identified core regions are involved in inducing GmCaM-4 expression in the heterologous system. Finally, a transient expression assay of Arabidopsis protoplasts showed that the GmCaM-4 promoter produced greater levels of GUS activity than did the CaMV35S promoter after pathogen or NaCl treatments, suggesting that the GmCaM-4 promoter may be useful in the production of conditional gene expression systems.
authors.affiliation: Division of Applied Life Science, Plant Molecular Biology and Biotechnology Research Center and Environmental Biotechnology National Core Research Center, Gyeongsang National University, Jinju, Korea. hcpark@gnu.ac.kr
authors.forename: Hyeong Cheol
authors.initials: HC
authors.lastname: Park
authors.forename: Man Lyang
authors.initials: ML
authors.lastname: Kim
authors.forename: Yun Hwan
authors.initials: YH
authors.lastname: Kang
authors.forename: Jae Cheol
authors.initials: JC
authors.lastname: Jeong
authors.forename: Mi Sun
authors.initials: MS
authors.lastname: Cheong
authors.forename: Wonkyun
authors.initials: W
authors.lastname: Choi
authors.forename: Sang Yeol
authors.initials: SY
authors.lastname: Lee
authors.forename: Moo Je
authors.initials: MJ
authors.lastname: Cho
authors.forename: Min Chul
authors.initials: MC
authors.lastname: Kim
authors.forename: Woo Sik
authors.initials: WS
authors.lastname: Chung
authors.forename: Dae-Jin
authors.initials: DJ
authors.lastname: Yun
mesh_terms: D001665:Binding Sites; D002147:Calmodulin / Q000096:biosynthesis / Q000235:genetics*; D004268:DNA-Binding Proteins; D018506:Gene Expression Regulation, Plant; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics; D030821:Plants, Genetically Modified; D011401:Promoter Regions, Genetic; D020033:Protein Isoforms; D017422:Sequence Analysis, DNA; D013025:Glycine max / Q000235:genetics* / Q000378:metabolism; D013312:Stress, Physiological; D014026:Nicotiana / Q000235:genetics* / Q000378:metabolism; D015533:Transcriptional Activation
chemical_list: D002147:Calmodulin; D004268:DNA-Binding Proteins; D010940:Plant Proteins; D020033:Protein Isoforms