title: A game with many players: control of gdh transcription in Corynebacterium glutamicum.
abstract: Glutamate dehydrogenase is a central enzyme connecting nitrogen and carbon metabolism via its precursors ammonium and oxoglutarate and its product glutamate. In Corynebacterium glutamicum glutamate dehydrogenase is especially important, since it is a key enzyme for the biotechnological production of the flavour enhancer L-glutamate. In this study, the regulation of gdh transcription was investigated. While originally described as a constitutively expressed gene, we could show that gdh transcription is highly variable depending on environmental conditions. Regulation of the gdh gene by the C. glutamicum nitrogen control protein AmtR was analyzed in detail in this study.
authors.affiliation: Lehrstuhl für Mikrobiologie, Friedrich-Alexander-Universität Erlangen-Nürnberg, Staudtstr. 5, 91058 Erlangen, Germany.
authors.forename: Eva
authors.initials: E
authors.lastname: Hänssler
authors.forename: Tim
authors.initials: T
authors.lastname: Müller
authors.forename: Katrin
authors.initials: K
authors.lastname: Palumbo
authors.forename: Mirek
authors.initials: M
authors.lastname: Patek
authors.forename: Melanie
authors.initials: M
authors.lastname: Brocker
authors.forename: Reinhard
authors.initials: R
authors.lastname: Krämer
authors.forename: Andreas
authors.initials: A
authors.lastname: Burkovski
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D048230:Corynebacterium glutamicum / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development; D024202:Electrophoretic Mobility Shift Assay; D015964:Gene Expression Regulation, Bacterial; D005969:Glutamate Dehydrogenase / Q000235:genetics* / Q000378:metabolism; D009584:Nitrogen / Q000378:metabolism; D011401:Promoter Regions, Genetic; D012097:Repressor Proteins / Q000378:metabolism; D013312:Stress, Physiological; D013696:Temperature; D014158:Transcription, Genetic
chemical_list: C414398:AmtR protein, Corynebacterium glutamicum; D001426:Bacterial Proteins; D012097:Repressor Proteins; D005969:Glutamate Dehydrogenase; D009584:Nitrogen