Hyperbranched polysiloxysilane nanoparticles: surface charge control of nonviral gene delivery vectors and nanoprobes.
New hyperbranched polysiloxysilane (HBPS) materials containing terminal carboxylic acid and quaternary ammonium groups were designed and synthesized to obtain fluorescent-dye-encapsulated nanoparticles. These polymers exhibited desirable characteristics, including amphiphilicity for nanoparticle formation, and contained various terminal groups for surface-charge control on the nanoparticles or for further bioconjugation for targeted imaging. Nanoprobes composed of polysiloxysilane nanoparticles encapsulating two-photon dyes were also prepared for optical bioimaging with controlled surface charge density (zeta potential) for modulation of cellular uptake. Intracellular delivery of these structurally similar polysiloxysilane nanoparticles, with substantially different surface charges, was investigated using confocal and two-photon fluorescence microscopy as well as flow cytometry. Finally, the use of these nanoparticles as efficient gene delivery vectors was demonstrated by means of in vitro transfection study using beta-galactosidase plasmid and pEGFP-N1 plasmid and the most efficient combination was obtained using HBPS-CN30:70.
Kim
Won Jin
WJ
Institute for Lasers, Photonics, and Biophotonics, University at Buffalo, The State University of New York, Buffalo, NY 14260-3000, USA.
Bonoiu
Adela C
AC
Hayakawa
Teruaki
T
Xia
Cheng
C
Kakimoto
Masa-Aki
MA
Pudavar
Haridas E
HE
Lee
Kwang-Sup
KS
Prasad
Paras N
PN
D000818:Animals; D019556:COS Cells; D002470:Cell Survival / Q000187:drug effects; D002522:Chlorocebus aethiops; D004337:Drug Carriers / Q000138:chemical synthesis / Q000493:pharmacokinetics; D016503:Drug Delivery Systems / Q000379:methods*; D005456:Fluorescent Dyes / Q000737:chemistry / Q000493:pharmacokinetics*; D018014:Gene Transfer Techniques; D005822:Genetic Vectors; D049452:Green Fluorescent Proteins / Q000235:genetics; D006367:HeLa Cells; D006801:Humans; D008856:Microscopy, Fluorescence; D008956:Models, Chemical; D015394:Molecular Structure; D053758:Nanoparticles / Q000008:administration & dosage / Q000737:chemistry*; D010957:Plasmids / Q000235:genetics; D012833:Siloxanes / Q000008:administration & dosage / Q000138:chemical synthesis* / Q000737:chemistry; D014162:Transfection / Q000379:methods*; D001616:beta-Galactosidase / Q000235:genetics
D004337:Drug Carriers; D005456:Fluorescent Dyes; D012833:Siloxanes; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins; D001616:beta-Galactosidase