title: Differential diagnosis of orf viruses by a single-step PCR.
abstract: The complete nucleotide sequence of the A32L gene (named after vaccinia virus, corresponding with open reading frame 108 of the orf virus and encoding an ATPase) of the orf virus was studied using samples of orf virus from infected goats, which were collected from six outbreaks in central Taiwan. DNA sequence analysis of the A32L genes of these and isolates from other countries showed sequence heterogeneity (base pair variation and deletion) in the 3'-terminal regions. This finding led to the development of a polymerase chain reaction (PCR) method for the rapid differential diagnosis of orf virus infections, and the results demonstrated that this was an easy and reliable method for genotyping of orf viruses.
authors.affiliation: Department of Veterinary Medicine, National Chung-Hsing University, Taichung, Taiwan.
authors.forename: Kun-Wei
authors.initials: KW
authors.lastname: Chan
authors.forename: Wei-Li
authors.initials: WL
authors.lastname: Hsu
authors.forename: Chi-Young
authors.initials: CY
authors.lastname: Wang
authors.forename: Cheng-Hsiung
authors.initials: CH
authors.lastname: Yang
authors.forename: Fong-Yuan
authors.initials: FY
authors.lastname: Lin
authors.forename: Songkhla
authors.initials: S
authors.lastname: Chulakasian
authors.forename: Min-Liang
authors.initials: ML
authors.lastname: Wong
mesh_terms: D000251:Adenosine Triphosphatases / Q000235:genetics; D000818:Animals; D001483:Base Sequence; D003937:Diagnosis, Differential; D004196:Disease Outbreaks; D004474:Ecthyma, Contagious / Q000175:diagnosis* / Q000453:epidemiology / Q000821:virology*; D005838:Genotype; D006041:Goats; D008969:Molecular Sequence Data; D009923:Orf virus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000379:methods*; D011110:Polymorphism, Genetic; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D013624:Taiwan; D014764:Viral Proteins / Q000235:genetics
chemical_list: D014764:Viral Proteins; D000251:Adenosine Triphosphatases