Distribution and evolution of ferripyoverdine receptors in Pseudomonas aeruginosa.
Pseudomonas aeruginosa is a ubiquitous gram-negative bacterium, which is also able to cause severe opportunistic infections in humans. The colonization of the host is importantly affected by the production of the high-affinity iron (III) scavenging peptidic siderophore pyoverdine. The species P. aeruginosa can be divided into three subgroups ('siderovars'), each characterized by the production of a specific pyoverdine and receptor (FpvA). We used a multiplex PCR to determine the FpvA siderovar on 345 P. aeruginosa strains from environmental or clinical origin. We found about the same proportion of each type in clinical strains, while FpvA type I was slightly over-represented (49%) in environmental strains. Our multiplex PCR also detected the presence or absence of an additional receptor for type I pyoverdine (FpvB). The fpvB gene was in fact present in the vast majority of P. aeruginosa strains (93%), regardless of their siderovar or their origin. Finally, molecular analyses of fpvA and fpvB genes highlighted a complex evolutionary history, probably linked to the central role of iron acquisition in the ecology and virulence of P. aeruginosa.
Bodilis
Josselin
J
Université de Rouen, Laboratoire M2C, UMR CNRS 6143, groupe microbiologie, Bâtiment IRESE B, UFR des Sciences, 76821 Mont Saint Aignan, France.
Ghysels
Bart
B
Osayande
Julie
J
Matthijs
Sandra
S
Pirnay
Jean-Paul
JP
Denayer
Sarah
S
De Vos
Daniel
D
Cornelis
Pierre
P
D001425:Bacterial Outer Membrane Proteins / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D019143:Evolution, Molecular; D005798:Genes, Bacterial; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D011550:Pseudomonas aeruginosa / Q000145:classification* / Q000235:genetics
D001425:Bacterial Outer Membrane Proteins; C082326:FpvA protein, Pseudomonas aeruginosa