Gene expression and enzyme activity of mitochondrial proteins in irradiated rainbow trout (Oncorhynchus mykiss, Walbaum) tissues in vitro.
In recent years ethical, legislative and economic pressures have created a renewed interest in the development of alternatives to in vivo animal experiments. In vitro studies, particularly those using cell cultures, have been used increasingly as tools to assess the degree of toxicity associated with or present in particular environments. While cell cultures are useful to give relative toxicity values, genotypic and phenotypic integrity may be compromised in the continuous artificial environment they experience. In addition, cell cultures lack the complexity of functional organs and thus do not truly represent the effects that toxins exert on organ and organism functionality. In this study, ex vivo tissue cultures of rainbow trout gill, skin and spleen samples were analyzed for variation of expression in genes associated with oxidative phosphorylation after exposure to ionizing radiation. Significant radiation-induced changes in gene expression and enzyme activity associated with the mitochondrial oxidative phosphorylation process were identified. The tissues examined in this study demonstrated an exposure threshold at which radiation dose stimulates an alteration in the regulatory activity of mitochondrial-associated genes. Spleen tissues exposed to low levels of radiation (0.1 Gy) appeared most sensitive whereas skin tissues proved least sensitive, reacting only to higher doses (>1 Gy). We propose this investigative approach as an innovative alternative to in vivo studies because it identifies toxic exposure in vitro and could significantly reduce the number of live-animal toxicity tests required.
O'Dowd
Colm
C
Radiation and Environmental Science Centre, Focas Institute, Dublin Institute of Technology, Dublin 8, Ireland. colm.odowd@nuigalway.ie
Mothersill
Carmel E
CE
Cairns
Michael T
MT
Austin
Brian
B
Lyng
Fiona M
FM
McClean
Brendan
B
Talbot
Anita
A
Murphy
James E J
JE
D000818:Animals; D002950:Citrate (si)-Synthase / Q000378:metabolism; D017931:DNA Primers / Q000378:metabolism; D005399:Fishes; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000528:radiation effects*; D005880:Gills / Q000528:radiation effects; D008928:Mitochondria / Q000378:metabolism*; D024101:Mitochondrial Proteins / Q000378:metabolism*; D008954:Models, Biological; D017686:Oncorhynchus mykiss; D010100:Oxygen / Q000378:metabolism; D010766:Phosphorylation; D012867:Skin / Q000528:radiation effects; D013154:Spleen / Q000528:radiation effects
D017931:DNA Primers; D024101:Mitochondrial Proteins; D002950:Citrate (si)-Synthase; D010100:Oxygen