How to predict implantation? No correlation between embryonic aneuploidy and soluble human leukocyte antigen G-concentrations.
OBJECTIVE
To determine if soluble human leukocyte antigen-G (sHLA-G) concentrations in spent culture media may assist in identifying the normal embryo for implantation.


METHODS
Prospective blinded comparative study.


METHODS
Reproductive genetic and reproductive medicine centers.


METHODS
One hundred and sixteen embryos obtained from eight patients undergoing in vitro fertilization (IVF) with preimplantation genetic diagnosis (PGD).


METHODS
Culture media obtained 2 days after fertilization were analyzed for sHLA-G concentrations using an enzyme-linked immunosorbent assay (ELISA) assay. A sHLA-G concentration of >or=1.9 mIU/mL was considered a positive predictor for successful implantation. Polar bodies and blastomeres from day-3 embryos were tested by PGD for 5 to 11 chromosomes: 8, 9, 13, 15, 16, 17, 18, 21, 22, X, and Y.


METHODS
The results of the sHLA-G concentrations were compared with the results of the PGD analyses.


RESULTS
We found an sHLA-G concentration >or=1.9 mIU/mL in 48% (56 out of 116) and normal PGD results in 52% (57 out of 116) of embryos. Of the embryos with normal PGD results, 46% (26 out of 57) had sHLA-G concentrations >or=1.9 mIU/mL. Among the embryos with sHLA-G >or=1.9 mIU/mL, 46% (26 out of 56) had normal PGD results, and 21% of embryos displayed both normal PGD results and sHLA-G >or=1.9 mIU/mL.


CONCLUSIONS
No correlation between concentrations of sHLA-G in embryo culture media and PGD results of an embryo's aneuploidy were observed.
Coulam
Carolyn B
CB
Rinehart Center for Reproductive Medicine, Evanston, Illinois, USA. cbcoulam@aol.com
Roussev
Roumen G
RG
Lerner
Sveltana
S
Zlatopolsky
Zev
Z
Ilkevitch
Yuri
Y
Tur-Kaspa
Ilan
I
D000782:Aneuploidy; D001757:Blastomeres / Q000502:physiology; D002869:Chromosome Aberrations / Q000706:statistics & numerical data; D002877:Chromosomes, Human / Q000235:genetics; D041321:Chromosomes, Human, X / Q000235:genetics; D041322:Chromosomes, Human, Y / Q000235:genetics; D047108:Embryonic Development / Q000502:physiology*; D005260:Female; D005307:Fertilization in Vitro; D006680:HLA Antigens / Q000097:blood*; D059951:HLA-G Antigens; D015395:Histocompatibility Antigens Class I / Q000097:blood*; D006801:Humans; D011237:Predictive Value of Tests; D011247:Pregnancy; D011256:Pregnancy Outcome; D019836:Preimplantation Diagnosis / Q000379:methods*
D006680:HLA Antigens; D059951:HLA-G Antigens; D015395:Histocompatibility Antigens Class I