title: Proteolytic fragments of chromogranins A and B represent major soluble components of chromaffin granules, illustrated by two-dimensional proteomics with NH(2)-terminal Edman peptide sequencing and MALDI-TOF MS.
abstract: Neuroendocrine chromaffin granules of adrenal medulla represent regulated secretory vesicles that secrete neuropeptides and catecholamines which mediate cell-cell communication for physiological functions. This study addressed the identification of the major proteins in these secretory vesicles that provide dynamic storage and secretion of bioactive molecules. Proteins of the soluble compartment of the vesicles were separated by two-dimensional gels and subjected to NH(2)-terminal Edman sequencing for identification and determination of NH(2)-termini. Results showed that proteolytic fragments of chromogranin A (CgA) and chromogranin B (CgB) represent the major proteins of these secretory vesicles. These fragments resulted from cleavage of their respective precursor proteins at dibasic and monobasic sites, which is consistent with the known cleavage specificities of prohormone processing enzymes. MALDI-TOF MS analyses of protein spots similar in molecular weight that possessed a range of pI values were represented by molecular forms of CgA and CgB proteins. These findings indicate the high prevalence of endogenous CgA and CgB proteolytic fragments that function in chromaffin secretory vesicles for release of bioactive molecules for cell-cell communication.
authors.affiliation: Department of Medicine, University of California at San Diego, La Jolla, California 92093, USA.
authors.forename: Jean C
authors.initials: JC
authors.lastname: Lee
authors.forename: Vivian
authors.initials: V
authors.lastname: Hook
mesh_terms: D000313:Adrenal Medulla / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D002837:Chromaffin Granules / Q000378:metabolism*; D053379:Chromogranin A / Q000737:chemistry* / Q000378:metabolism; D053378:Chromogranin B / Q000737:chemistry* / Q000378:metabolism; D008969:Molecular Sequence Data; D009943:Organophosphorus Compounds / Q000737:chemistry*; D040901:Proteomics / Q000379:methods*; D016415:Sequence Alignment; D020539:Sequence Analysis, Protein / Q000379:methods*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
chemical_list: C043517:2-(4-isothiocyanatophenoxy)-1,3,2-dioxaphosphinene 2-oxide; D053379:Chromogranin A; D053378:Chromogranin B; D009943:Organophosphorus Compounds