title: Third harmonic generation microscopy.
abstract: Third harmonic generation microscopy is used to make dynamical images of living systems for the first time. A 100 fs excitation pulse at 1.2 aem results in a 400 nm signal which is generated directly within the specimen. Chara plant rhizoids have been imaged, showing dynamic plant activity, and non-fading image characteristics even with continuous viewing, indicating prolonged viability under these THG-imaging conditions.
authors.forename: J
authors.initials: J
authors.lastname: Squier
authors.forename: M
authors.initials: M
authors.lastname: Muller
authors.forename: G
authors.initials: G
authors.lastname: Brakenhoff
authors.forename: K R
authors.initials: KR
authors.lastname: Wilson