Identifying new components participating in the secondary cell wall formation of vessel elements in zinnia and Arabidopsis.
Xylem vessel elements are hollow cellular units that assemble end-to-end to form a continuous vessel throughout the plant body; the xylem vessel is strengthened by the xylem elements' reinforced secondary cell walls (SCWs). This work aims to unravel the contribution of unknown actors in xylem vessel differentiation using the model in vitro cell culture system of Zinnia elegans differentiating cell cultures and the model in vivo system of Arabidopsis thaliana plants. Tracheary Element Differentiation-Related6 (TED6) and TED7 were selected based on an RNA interference (RNAi) screen in the Zinnia system. RNAi reduction of TED6 and 7 delayed tracheary element (TE) differentiation and co-overexpression of TED6 and 7 increased TE differentiation in cultured Zinnia cells. Arabidopsis TED6 and 7 were expressed preferentially in differentiating vessel elements in seedlings. Aberrant SCW formation of root vessel elements was induced by transient RNAi of At TED7 alone and enhanced by inhibition of both TED6 and 7. Protein-protein interactions were demonstrated between TED6 and a subunit of the SCW-related cellulose synthase complex. Our strategy has succeeded in finding two novel components in SCW formation and has opened the door for in-depth analysis of their molecular functions.
Endo
Satoshi
S
RIKEN Plant Science Center, Yokohama, Kanagawa 230-0045, Japan.
Pesquet
Edouard
E
Yamaguchi
Masatoshi
M
Tashiro
Gen
G
Sato
Mayuko
M
Toyooka
Kiminori
K
Nishikubo
Nobuyuki
N
Udagawa-Motose
Makiko
M
Kubo
Minoru
M
Fukuda
Hiroo
H
Demura
Taku
T
D017360:Arabidopsis / Q000378:metabolism / Q000502:physiology / Q000648:ultrastructure*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology; D019659:Asteraceae / Q000378:metabolism / Q000502:physiology / Q000648:ultrastructure*; D002473:Cell Wall / Q000378:metabolism*; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D034622:RNA Interference; D012333:RNA, Messenger / Q000378:metabolism; D052584:Xylem / Q000254:growth & development / Q000378:metabolism / Q000648:ultrastructure*
D029681:Arabidopsis Proteins; D010940:Plant Proteins; D012333:RNA, Messenger; C543130:TED6 protein, Arabidopsis; C543131:TED7 protein, Arabidopsis