Development of the osteoblastic phenotype in human alveolar bone-derived cells grown on a collagen type I-coated titanium surface.
OBJECTIVE
The aim of this study was to evaluate the development of the osteoblastic phenotype in human alveolar bone-derived cells grown on collagen type I-coated titanium (Ti) surface (Col-Ti) obtained by plasma deposition acrylic acid grafting compared with machined Ti (M-Ti).


METHODS
Osteoblastic cells were cultured until subconfluence and subcultured on Col-Ti and M-Ti for periods of up to 21 days.


RESULTS
Cultures grown on Col-Ti and M-Ti exhibited similar cell morphology. Cell adhesion, total protein content, and alkaline phosphatase (ALP) activity were not affected by Ti surface modification in all evaluated periods. Growth analyses indicated that there were significantly more cells in cultures grown on Col-Ti at day 3. Runt-related transcription factor 2 (Runx2), osteopontin (OPN), and osteoprotegerin (OPG) mRNA expression of cells subcultured on Col-Ti was higher, whereas collagen type I (COL) was lower compared with M-Ti. Ti surface modification neither affected the osteocalcin (OC), ALP and receptor activator of NF-kappaB ligand (RANKL) mRNA expression nor the calcium content extracted from mineralized matrix.


CONCLUSIONS
These results demonstrated that Col-Ti favours cell growth during the proliferative phase (day 3) and osteoblastic differentiation, as demonstrated by changes in mRNA expression profile during the matrix mineralization phase (day 14), suggesting that this Ti surface modification may affect the processes of bone healing and remodelling.
de Assis
Adriano Freitas
AF
Cell Culture Laboratory, School of Dentistry of Ribeirao Preto, University of Sao Paulo, SP, Brazil.
Beloti
Marcio Mateus
MM
Crippa
Grasiele Edilaine
GE
de Oliveira
Paulo Tambasco
PT
Morra
Marco
M
Rosa
Adalberto Luiz
AL
D000539:Alveolar Process / Q000166:cytology* / Q000378:metabolism; D001857:Bone Matrix / Q000378:metabolism; D002113:Calcification, Physiologic / Q000502:physiology*; D002448:Cell Adhesion / Q000502:physiology; D018929:Cell Culture Techniques; D002454:Cell Differentiation / Q000502:physiology; D049109:Cell Proliferation; D020099:Coated Materials, Biocompatible / Q000378:metabolism*; D024042:Collagen Type I / Q000378:metabolism*; D006801:Humans; D016348:Osseointegration / Q000502:physiology; D010006:Osteoblasts / Q000166:cytology* / Q000378:metabolism; D012333:RNA, Messenger / Q000032:analysis; D013499:Surface Properties; D014025:Titanium
D020099:Coated Materials, Biocompatible; D024042:Collagen Type I; D012333:RNA, Messenger; D014025:Titanium