title: A highly sensitive fluorescence probe for fast thiol-quantification assay of glutathione reductase.
abstract: Fast detection of cellular thiols in aqueous medium was achieved using a newly developed fluorescence probe (see picture). Based on this probe, a high-throughput fluorescence assay for glutathione reductase was developed.
authors.affiliation: Department of Chemical Biology and State Key Laboratory of Elemento-organic Chemistry, Nankai University, Tianjin, 300071, China.
authors.forename: Long
authors.initials: L
authors.lastname: Yi
authors.forename: Heyang
authors.initials: H
authors.lastname: Li
authors.forename: Lu
authors.initials: L
authors.lastname: Sun
authors.forename: Liangliang
authors.initials: L
authors.lastname: Liu
authors.forename: Caihong
authors.initials: C
authors.lastname: Zhang
authors.forename: Zhen
authors.initials: Z
authors.lastname: Xi
mesh_terms: D002460:Cell Line; D003374:Coumarins / Q000138:chemical synthesis / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry*; D005470:Fluorometry / Q000379:methods; D005980:Glutathione Reductase / Q000032:analysis*; D006801:Humans; D013438:Sulfhydryl Compounds / Q000032:analysis*
chemical_list: D003374:Coumarins; D005456:Fluorescent Dyes; D013438:Sulfhydryl Compounds; D005980:Glutathione Reductase