title: [A procedure for determination of acid mannosidase activities in biological fluids].
abstract: A procedure has been developed for simultaneous determination of the activities of alpha-D- and beta-D-mannosidase in the biological fluids from the quantity of free 4-nitrophenol. The latter is released via enzymatic degradation of substrates of 4-nitrophenyl-alpha-D-mannose and 4-nitrophenyl-beta-D-mannose in individual incubation tests.
authors.forename: P N
authors.initials: PN
authors.lastname: Sharaev
authors.forename: A B
authors.initials: AB
authors.lastname: Zamiatin
authors.forename: R R
authors.initials: RR
authors.lastname: Kil'diiarova
authors.forename: N Sh
authors.initials: NSh
authors.lastname: Parulava
authors.forename: Iu V
authors.initials: IuV
authors.lastname: Khobta
mesh_terms: D000293:Adolescent; D000328:Adult; D000818:Animals; D053627:Asthenozoospermia / Q000175:diagnosis / Q000201:enzymology*; D002648:Child; D004382:Duodenitis / Q000175:diagnosis / Q000201:enzymology*; D005756:Gastritis / Q000175:diagnosis / Q000201:enzymology*; D006801:Humans; D008297:Male; D009596:Nitrophenols / Q000378:metabolism; D051381:Rats; D013094:Spermatozoa / Q000201:enzymology*; D055815:Young Adult; D043323:alpha-Mannosidase / Q000032:analysis* / Q000097:blood / Q000378:metabolism; D044902:beta-Mannosidase / Q000032:analysis* / Q000097:blood / Q000378:metabolism
chemical_list: D009596:Nitrophenols; D043323:alpha-Mannosidase; D044902:beta-Mannosidase; C024836:4-nitrophenol