Ostryopsis davidiana seedlings inoculated with ectomycorrhizal fungi facilitate formation of mycorrhizae on Pinus tabulaeformis seedlings.
Reforestation in China is important for reversing anthropogenic activities that degrade the environment. Pinus tabulaeformis is desired for these activities, but survival and growth of seedlings can be hampered by lack of ectomycorrhizae. When outplanted in association with Ostryopsis davidiana plants on reforestation sites, P. tabulaeformis seedlings become mycorrhizal and survival and growth are enhanced; without O. davidiana, pines often remain without mycorrhizae and performance is poorer. To better understand this relationship, we initiated an experiment using rhizoboxes that restricted root and tested the hypothesis that O. davidiana seedlings facilitated ectomycorrhizae formation on P. tabulaeformis seedlings through hyphal contact. We found that without O. davidiana seedlings, inocula of five indigenous ectomycorrhizal fungi were unable to grow and associate with P. tabulaeformis seedlings. Inocula placed alongside O. davidiana seedlings, however, resulted in enhanced growth and nutritional status of O. davidiana and P. tabulaeformis seedlings, and also altered rhizosphere pH and phosphatase activity. We speculate that these species form a common mycorrhizal network and this association enhances outplanting performance of P. tabulaeformis seedlings used for forest restoration.
Bai
Shu-Lan
SL
College of Forestry, Inner Mongolia Agricultural University, Hohhot, 010019, China.
Li
Guo-Lei
GL
Key Laboratory for Silviculture and Conservation, Ministry of Education, Beijing Forestry University, Beijing, 100083, China. glli226@163.com.
Liu
Yong
Y
Key Laboratory for Silviculture and Conservation, Ministry of Education, Beijing Forestry University, Beijing, 100083, China.
Kasten Dumroese
R
R
USDA Forest Service, Rocky Mountain Research Station, Moscow, ID, 83843-4211, USA.
Lv
Rui-Heng
RH
Key Laboratory for Silviculture and Conservation, Ministry of Education, Beijing Forestry University, Beijing, 100083, China.
D029401:Betulaceae / Q000382:microbiology*; D002681:China; D006863:Hydrogen-Ion Concentration; D038821:Mycorrhizae / Q000254:growth & development*; D010744:Phosphoric Monoester Hydrolases / Q000378:metabolism; D028223:Pinus / Q000254:growth & development* / Q000382:microbiology*; D036226:Seedlings / Q000382:microbiology; D012987:Soil / Q000032:analysis
D012987:Soil; D010744:Phosphoric Monoester Hydrolases