[Detection of miR-122a and miR-224 expression in hepatocellular carcinoma by real-time fluorescence quantitative RT-PCR].
OBJECTIVE
To detect the expressions of miR-122 and miR-224 in hepatocellular carcinoma (HCC) by real-time fluorescence quantitative RT-PCR and investigate the significance of miRNAs in early diagnosis of HCC.


METHODS
2(-Delta Delta CT) method was used for quantitative analysis of the expression pattern of miR-122 and miR-224 in 35 HCC and adjacent normal tissues. All the quantitative results were confirmed by Northern blotting.


RESULTS
Compared with adjacent normal tissues, the HCC tissues showed significant miR-122 down-regulation (P<0.01) and miR-224 over-expression (P<0.001).


CONCLUSIONS
HCC has obvious alteration in the expression patterns of miR-122 and miR-224, and real-time fluorescence quantitative RT-PCR provide a new means for early, efficient, and accurate diagnosis of HCC.
Liu
Zhuo-ran
ZR
Department of Clinical Laboratory, Second Affiliated Hospital, University of South China, Hengyang 421001, China. LZr9656@163.com
Chen
Xiao-wei
XW
Qiao
Xin-hui
XH
Wang
Rong
R
Xiang
Zheng
Z
D000328:Adult; D000368:Aged; D001483:Base Sequence; D015152:Blotting, Northern; D006528:Carcinoma, Hepatocellular / Q000175:diagnosis / Q000235:genetics*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008113:Liver Neoplasms / Q000175:diagnosis / Q000235:genetics*; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013050:Spectrometry, Fluorescence; D013997:Time Factors
C531907:MIRN122 microRNA, human; C546140:MIRN224 microRNA, human; D035683:MicroRNAs