BAFF and APRIL expression in B-cell chronic lymphocytic leukemia: correlation with biological and clinical features.
B-cell activating factor (BAFF) and a proliferation-inducing ligand (APRIL) are involved in normal B cell survival and differentiation. We analyzed BAFF and APRIL plasma levels in patients with B-cell chronic lymphocytic leukemia (B-CLL). We also tested intracellular BAFF and APRIL expression in B-CLL and evaluated their prognostic relevance. The percentage of leukemic B cells with intracellular APRIL or BAFF expression correlated significantly with adverse prognostic factors such as ZAP-70 and CD38. Moreover, we found a close relationship between LPL protein expression or LPL/ADAM29 MFI ratio and proportion of B-CLL cells with intracellular BAFF or APRIL expression. Furthermore, patients with a low percentage of leukemic cells with intracellular BAFF or APRIL expression had a significantly longer overall survival than those with a high proportion of APRIL or BAFF positive leukemic cells.
Bojarska-Junak
Agnieszka
A
Department of Clinical Immunology, Medical University of Lublin, Jaczewskiego 8, 20-954 Lublin, Poland. agnieszkajunak@poczta.onet.pl
Hus
Iwona
I
Chocholska
Sylwia
S
Wasik-Szczepanek
Ewa
E
Sieklucka
Małgorzata
M
Dmoszyńska
Anna
A
Roliński
Jacek
J
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D053264:B-Cell Activating Factor / Q000097:blood / Q000235:genetics* / Q000378:metabolism; D001402:B-Lymphocytes / Q000473:pathology; D005260:Female; D006801:Humans; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000097:blood / Q000235:genetics* / Q000401:mortality / Q000473:pathology; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D012334:RNA, Neoplasm / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016019:Survival Analysis; D017741:Survivors; D053300:Tumor Necrosis Factor Ligand Superfamily Member 13 / Q000097:blood / Q000235:genetics* / Q000378:metabolism
D053264:B-Cell Activating Factor; D012334:RNA, Neoplasm; C114631:TNFSF13 protein, human; C505719:TNFSF13B protein, human; D053300:Tumor Necrosis Factor Ligand Superfamily Member 13