title: Generation of retinal cells from mouse and human induced pluripotent stem cells.
abstract: We previously reported a technique for generating retinal pigment epithelia (RPE) and putative photoreceptors from embryonic stem (ES) cells. Here we tested whether our procedure can promote retinal differentiation of mouse and human induced pluripotent stem cells (iPSCs). Treating iPSCs with Wnt and Nodal antagonists in suspension culture induced expression of markers of retinal progenitor cells and generated RPE cells. Subsequently, treatment with retinoic acid and taurine generated cells positive for photoreceptor markers in all but one human cell lines. We propose that iPSCs can be induced to differentiate into retinal cells which have a possibility to be used as patient-specific donor cells for transplantation therapies.
authors.affiliation: Laboratory for Retinal Regeneration, RIKEN Center for Developmental Biology, 2-2-3 Minatojima-minamimachi, Chuo-ku, Kobe 650-0047, Japan.
authors.forename: Yasuhiko
authors.initials: Y
authors.lastname: Hirami
authors.forename: Fumitaka
authors.initials: F
authors.lastname: Osakada
authors.forename: Kazutoshi
authors.initials: K
authors.lastname: Takahashi
authors.forename: Keisuke
authors.initials: K
authors.lastname: Okita
authors.forename: Shinya
authors.initials: S
authors.lastname: Yamanaka
authors.forename: Hanako
authors.initials: H
authors.lastname: Ikeda
authors.forename: Nagahisa
authors.initials: N
authors.lastname: Yoshimura
authors.forename: Masayo
authors.initials: M
authors.lastname: Takahashi
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques; D002454:Cell Differentiation / Q000502:physiology*; D002460:Cell Line; D053595:Embryonic Stem Cells / Q000166:cytology; D005347:Fibroblasts / Q000166:cytology; D006801:Humans; D051379:Mice; D050814:Octamer Transcription Factor-3 / Q000235:genetics; D039904:Pluripotent Stem Cells / Q000166:cytology* / Q000502:physiology / Q000637:transplantation; D012160:Retina / Q000166:cytology* / Q000502:physiology*; D012162:Retinal Degeneration / Q000601:surgery; D055213:Retinal Pigment Epithelium / Q000166:cytology / Q000637:transplantation; D012867:Skin / Q000166:cytology; D014162:Transfection
chemical_list: D050814:Octamer Transcription Factor-3