Optimization of medium composition for butyric acid production by Clostridium thermobutyricum using response surface methodology.
The optimal medium for butyric acid production by Clostridium thermobutyricum in a shake flask culture was studied using statistical experimental design and analysis. The optimal composition of the fermentation medium for maximum butyric acid yield, as determined on the basis of a three-level four-factor Box-Behnken design (BBD), was obtained by response surface methodology (RSM). The high correlation between the predicted and observed values indicated the validity of the model. A maximum butyric acid yield of 12.05 g/l was obtained at K(2)HPO(4) 7.2 g/l, 34.9 g/l glucose, 20 g/l yeast extract, and 15 g/l acetate, which compared well to the predicated production of 12.13 g/l.
Zhang
Chun-Hui
CH
Key Laboratory of Biofuel, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Songling road 189, Qingdao 266071, China.
Ma
Yu-Jiu
YJ
Yang
Fang-Xiao
FX
Liu
Wei
W
Zhang
Yue-Dong
YD
D020148:Butyric Acid / Q000378:metabolism*; D003013:Clostridium / Q000378:metabolism*; D003470:Culture Media; D012984:Software
D003470:Culture Media; D020148:Butyric Acid