Morroniside protects human neuroblastoma SH-SY5Y cells against hydrogen peroxide-induced cytotoxicity.
Oxidative stress-induced cell damage has been implicated in a variety of neurodegenerative disorders. Morroniside, an iridorid glycoside isolated from Cornus officinalis Sieb. Et Zucc., has shown potent antioxidant properties. The present study investigated the protective actions of morroniside against the cytotoxicity produced by exposure to H(2)O(2) (300-500 microM) in SH-SY5Y cells. Intracellular accumulation of Ca(2+), and decreases in mitochondrial membrane potential (MMP) caused by added H(2)O(2) were reduced by morroniside. Incubation of cells with H(2)O(2) caused a marked decrease in superoxide dismutase (SOD) activity; this decrease was significantly inhibited by morroniside. In addition, the percentage of cells undergoing H(2)O(2)-induced apoptosis was decreased, dose dependently, in the presence of morroniside. These results suggest that morroniside has protective effects against oxidative stress-induced neurotoxic processes.
Wang
Wen
W
Department of Pharmacology, Xuanwu Hospital of Capital Medical University, Key Laboratory for Neurodegenerative Diseases of Ministry of Education, Beijing, China.
Sun
Fangling
F
An
Yi
Y
Ai
Houxi
H
Zhang
Li
L
Huang
Wenting
W
Li
Lin
L
D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D002118:Calcium / Q000378:metabolism; D045744:Cell Line, Tumor; D003603:Cytotoxins / Q000037:antagonists & inhibitors*; D004790:Enzyme Induction / Q000187:drug effects; D006027:Glycosides / Q000032:analysis / Q000494:pharmacology*; D006801:Humans; D006861:Hydrogen Peroxide / Q000633:toxicity*; D042541:Intracellular Space / Q000187:drug effects / Q000378:metabolism; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D009447:Neuroblastoma / Q000473:pathology*; D013482:Superoxide Dismutase / Q000096:biosynthesis / Q000378:metabolism
D003603:Cytotoxins; D006027:Glycosides; C488401:morroniside; D006861:Hydrogen Peroxide; D013482:Superoxide Dismutase; D002118:Calcium