Structure of TTHA1623, a novel metallo-beta-lactamase superfamily protein from Thermus thermophilus HB8.
TTHA1623 is a metallo-beta-lactamase superfamily protein from the extremely thermophilic bacterium Thermus thermophilus HB8. Homologues of TTHA1623 exist in a wide range of bacteria and archaea and one eukaryote, Giardia lamblia, but their function remains unknown. To analyze the structural properties of TTHA1623, the crystal structures of its iron-bound and zinc-bound forms have been determined to 2.8 and 2.2 A resolution, respectively. TTHA1623 possesses an alphabetabetaalpha-fold similar to that of other metallo-beta-lactamase superfamily proteins with glyoxalase II-type metal coordination. However, TTHA1623 exhibits a putative substrate-binding pocket with a unique shape.
Yamamura
Akihiro
A
Department of Applied Biological Chemistry, University of Tokyo, Yayoi, Bunkyo-ku, Japan.
Okada
Akitoshi
A
Kameda
Yasuhiro
Y
Ohtsuka
Jun
J
Nakagawa
Noriko
N
Ebihara
Akio
A
Nagata
Koji
K
Tanokura
Masaru
M
D018360:Crystallography, X-Ray; D008670:Metals / Q000737:chemistry* / Q000378:metabolism*; D008958:Models, Molecular; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D040681:Structural Homology, Protein; D016963:Thermus thermophilus / Q000201:enzymology* / Q000235:genetics; D001618:beta-Lactamases / Q000737:chemistry* / Q000145:classification / Q000235:genetics / Q000378:metabolism*
D008670:Metals; D001618:beta-Lactamases