Inhibitory effect of methanol extract of Rosa damascena Mill. flowers on alpha-glucosidase activity and postprandial hyperglycemia in normal and diabetic rats.
The effect of a methanol extract of Rosa damascena Mill. flowers was studied, in comparison to the alpha-glucosidase inhibitor acarbose, in normal and diabetic rats. The inhibition mode of this extract was examined by measuring enzyme activity in different concentrations of substrate for Lineweaver-Burk plot analysis. The results show that Rosa damascena extract has an intensive inhibitory effect on alpha-glucosidase. Its inhibition was found to be noncompetitive. Oral administration of this plant extract (100 to 1000 mg/kg body wt.) significantly decreased blood glucose after maltose loading in normal and diabetic rats in a dose-dependent manner. These results suggest that Rosa damascena might exert an anti-diabetic effect by suppressing carbohydrate absorption from the intestine and can reduce the postprandial glucose level.
Gholamhoseinian
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A
Department of Biochemistry, Medical School & Kerman Physiology Research Center, Kerman University of Medical Sciences, Kerman, Iran. agnajar@yahoo.com
Fallah
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H
Sharifi far
F
F
D020909:Acarbose / Q000494:pharmacology; D000818:Animals; D001786:Blood Glucose / Q000187:drug effects*; D003921:Diabetes Mellitus, Experimental / Q000188:drug therapy*; D065089:Glycoside Hydrolase Inhibitors; D007004:Hypoglycemic Agents / Q000032:analysis / Q000494:pharmacology* / Q000627:therapeutic use; D007700:Kinetics; D008297:Male; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology / Q000627:therapeutic use; D019518:Postprandial Period / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar; D027826:Rosa / Q000737:chemistry*
D001786:Blood Glucose; D065089:Glycoside Hydrolase Inhibitors; D007004:Hypoglycemic Agents; D010936:Plant Extracts; D020909:Acarbose