ANG II-AT1 receptor pathway is involved in the anti-fibrotic effect of beta-elemene.
To investigate the effects of beta-elemene on the ANG II-AT1 receptor pathway in rats with liver fibrosis, a model of hepatic fibrosis was induced by hypodermical injection of carbon tetrachloride (CCl4) into Wistar male rats. beta-elemene was intraperitonealy administered into the rats for 8 weeks (0.1 mL/100 g body weight per day). Masson staining was used to observe the liver fibrosis of rats and liver functions were measured by enzymatic kinetic analysis. The content of hydroxyproline in liver tissues was detected by specimen alkaline hydrolysis. The level of plasma ANG in blood II plasma was detected by radioimmunoassay. The expression of AT1R in rat liver were measured using reverse transcriptional-polymerase chain reaction and immunohistochemistry respectively. The results showed that beta-elemene could reduce the collagen disposition in liver and inhibit the progression of liver fibrosis. In addition, the levels of plasma ANG II and the expression of hepatic AT1R in rats with liver fibrosis were also suppressed by beta-elemene. It is concluded that the ANG II-AT1 receptor pathway plays an important role in the development of hepatic fibrosis and beta-elemene could down-regulate the levels of plasma ANG II and the expression of hepatic AT1R in rats with liver fibrosis.
Zhu
Rui
R
Department of Traditional Chinese Medicine, Huazhong University of Science and Technology, Wuhan, 430022, China. zhurui19830108@163.com
Yang
Ling
L
Shen
Lin
L
Ye
Jin
J
Liu
Jianguo
J
Hu
Shenjun
S
D000804:Angiotensin II / Q000378:metabolism*; D000818:Animals; D015536:Down-Regulation / Q000187:drug effects; D008103:Liver Cirrhosis / Q000188:drug therapy* / Q000378:metabolism*; D008297:Male; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D044140:Receptor, Angiotensin, Type 1 / Q000378:metabolism*; D012717:Sesquiterpenes / Q000494:pharmacology / Q000627:therapeutic use*
D044140:Receptor, Angiotensin, Type 1; D012717:Sesquiterpenes; C445979:beta-elemene; D000804:Angiotensin II