Tissue-specific extracellular matrix coatings for the promotion of cell proliferation and maintenance of cell phenotype.
Recent studies have shown that extracellular matrix (ECM) substitutes can have a dramatic impact on cell growth, differentiation and function. However, these ECMs are often applied generically and have yet to be developed for specific cell types. In this study, we developed tissue-specific ECM-based coating substrates for skin, skeletal muscle and liver cell cultures. Cellular components were removed from adult skin, skeletal muscle, and liver tissues, and the resulting acellular matrices were homogenized and dissolved. The ECM solutions were used to coat culture dishes. Tissue matched and non-tissue matched cell types were grown on these coatings to assess adhesion, proliferation, maintenance of phenotype and cell function at several time points. Each cell type showed better proliferation and differentiation in cultures containing ECM from their tissue of origin. Although subtle compositional differences in the three ECM types were not investigated in this study, these results suggest that tissue-specific ECMs provide a culture microenvironment that is similar to the in vivo environment when used as coating substrates, and this new culture technique has the potential for use in drug development and the development of cell-based therapies.
Zhang
Yuanyuan
Y
Wake Forest Institute for Regenerative Medicine, Wake Forest University School of Medicine, Medical Center Blvd., Winston-Salem, NC 27157, United States. yzhang@wfubmc.edu
He
Yujiang
Y
Bharadwaj
Shantaram
S
Hammam
Nevin
N
Carnagey
Kristen
K
Myers
Regina
R
Atala
Anthony
A
Van Dyke
Mark
M
D000818:Animals; D001672:Biocompatible Materials / Q000494:pharmacology*; D015153:Blotting, Western; D002448:Cell Adhesion / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D005109:Extracellular Matrix; D005434:Flow Cytometry; D006801:Humans; D007150:Immunohistochemistry; D008099:Liver / Q000166:cytology; D018482:Muscle, Skeletal / Q000166:cytology; D051381:Rats; D012867:Skin / Q000166:cytology
D001672:Biocompatible Materials