title: Detection of TT virus in HIV-1 exposed but uninfected individuals and in HIV-1 infected patients and its influence on CD4+ lymphocytes and viral load.
abstract: The TT virus (TTV) was detected for the first time in the serum of a patient with post-transfusion hepatitis of unknown origin. TTV was subsequently, also found in the serum of blood donors with no history of blood transfusion. In the present study, the percentage of TTV carriers among HIV-infected and noninfected patients was determined. The study was conducted to evaluate CD4 count and HIV viral load in 100 asymptomatic patients infected with HIV-1, 100 symptomatic patients with AIDS, 100 HIV-1 exposed but uninfected individuals and 100 normal healthy blood donors. In this work, the presence of TTV was investigated by nested-PCR. TTV was detected in 6% of normal donors, 12.5% of HIV-infected individuals and 21% of exposed individuals. The presence of TTV was statistically significant in the HIV-exposed individuals (21/100) compared with blood donors (6/100). Odds ratio=4.16 (95%CI 1.60-10.83). No inter-group relations were found for CD4 and CD8 counts or HIV viral load. In the symptomatic group, patients with TTV presented minor viral load. This work demonstrated that TTV was detected in HIV-exposed individuals and no relation was verified for CD4, CD8 and viral load in the asymptomatic and symptomatic HIV patients.
authors.affiliation: Department of Pathological Sciences, Biological Sciences Center, Londrina State University, Londrina, PR, Brazil.
authors.forename: Thiago Franco
authors.initials: TF
authors.lastname: Nasser
authors.forename: Karen
authors.initials: K
authors.lastname: Brajão de Oliveira
authors.forename: Edna Maria Vissoci
authors.initials: EM
authors.lastname: Reiche
authors.forename: Marla Karine
authors.initials: MK
authors.lastname: Amarante
authors.forename: Maria Helena
authors.initials: MH
authors.lastname: Pelegrinelli Fungaro
authors.forename: Maria Angelica Ehara
authors.initials: MA
authors.lastname: Watanabe
mesh_terms: D018791:CD4 Lymphocyte Count; D004266:DNA Virus Infections / Q000150:complications* / Q000453:epidemiology* / Q000821:virology; D015658:HIV Infections / Q000150:complications* / Q000276:immunology / Q000821:virology*; D015497:HIV-1 / Q000302:isolation & purification*; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods; D015995:Prevalence; D022783:Torque teno virus / Q000302:isolation & purification*; D019562:Viral Load