title: Toward cloning of the magnetotactic metagenome: identification of magnetosome island gene clusters in uncultivated magnetotactic bacteria from different aquatic sediments.
abstract: In this report, we describe the selective cloning of large DNA fragments from magnetotactic metagenomes from various aquatic habitats. This was achieved by a two-step magnetic enrichment which allowed the mass collection of environmental magnetotactic bacteria (MTB) virtually free of nonmagnetic contaminants. Four fosmid libraries were constructed and screened by end sequencing and hybridization analysis using heterologous magnetosome gene probes. A total of 14 fosmids were fully sequenced. We identified and characterized two fosmids, most likely originating from two different alphaproteobacterial strains of MTB that contain several putative operons with homology to the magnetosome island (MAI) of cultivated MTB. This is the first evidence that uncultivated MTB exhibit similar yet differing organizations of the MAI, which may account for the diversity in biomineralization and magnetotaxis observed in MTB from various environments.
authors.affiliation: LMU Department Biology I, Planegg-Martinsried, Germany.
authors.forename: Christian
authors.initials: C
authors.lastname: Jogler
authors.forename: Wei
authors.initials: W
authors.lastname: Lin
authors.forename: Anke
authors.initials: A
authors.lastname: Meyerdierks
authors.forename: Michael
authors.initials: M
authors.lastname: Kube
authors.forename: Emanuel
authors.initials: E
authors.lastname: Katzmann
authors.forename: Christine
authors.initials: C
authors.lastname: Flies
authors.forename: Yongxin
authors.initials: Y
authors.lastname: Pan
authors.forename: Rudolf
authors.initials: R
authors.lastname: Amann
authors.forename: Richard
authors.initials: R
authors.lastname: Reinhardt
authors.forename: Dirk
authors.initials: D
authors.lastname: Schüler
mesh_terms: D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification*; D023061:Gene Order; D005798:Genes, Bacterial; D044404:Genomic Islands; D008969:Molecular Sequence Data; D005810:Multigene Family; D015388:Organelles / Q000235:genetics*; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D014871:Water Microbiology
chemical_list: D004269:DNA, Bacterial