Direct toxicity assessment of toxic chemicals with electrochemical method.
Electrochemical measurement of respiratory chain activity is a rapid and reliable screening for the toxicity on microorganisms. Here, we investigated in-vitro effects of toxin on Escherichia coli (E. coli) that was taken as a model microorganism incubated with ferricyanide. The current signal of ferrocyanide effectively amplified by ultramicroelectrode array (UMEA), which was proven to be directly related to the toxicity. Accordingly, a direct toxicity assessment (DTA) based on chronoamperometry was proposed to detect the effect of toxic chemicals on microorganisms. The electrochemical responses to 3,5-dichlorophenol (DCP) under the incubation times revealed that the toxicity reached a stable level at 60 min, and its 50% inhibiting concentration (IC50) was estimated to be 8.0 mg L(-1). At 60 min incubation, the IC50 values for KCN and As2O3 in water samples were 4.9 mg L(-1) and 18.3 mg L(-1), respectively. But the heavy metal ions, such as Cu2+, Pb2+ and Ni2+, showed no obvious toxicity on E. coli. With the exception of Hg2+, it showed 40.0 mg L(-1) IC50 value when E. coli was exposed to its solution for 60 min. The lower sensitivity of DTA for the heavy metal ions could be attributed to the toxicological endpoint and the experimental conditions used. All results suggest that the DTA is a sensitive, rapid and inexpensive alternative to on-site water and wastewater toxic analysis.
Liu
Chang
C
College of Sciences, Northeastern University, Shenyang 110004, China.
Sun
Ting
T
Xu
Xiaolong
X
Dong
Shaojun
S
D000077237:Arsenic Trioxide; D001152:Arsenicals; D002733:Chlorophenols / Q000633:toxicity; D003485:Cyanates / Q000633:toxicity; D004563:Electrochemistry / Q000379:methods*; D004784:Environmental Monitoring; D004926:Escherichia coli / Q000737:chemistry; D005292:Ferricyanides / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D019216:Metals, Heavy / Q000633:toxicity; D010084:Oxidation-Reduction; D010087:Oxides / Q000633:toxicity; D013997:Time Factors; D014874:Water Pollutants, Chemical / Q000633:toxicity*
D001152:Arsenicals; D002733:Chlorophenols; D003485:Cyanates; D005292:Ferricyanides; D019216:Metals, Heavy; D010087:Oxides; D014874:Water Pollutants, Chemical; C116190:3,5-dichlorophenol; C024835:potassium cyanate; D000077237:Arsenic Trioxide