title: In vivo micro-CT imaging of rat brain glioma: a comparison with 3T MRI and histology.
abstract: The aim of this study was to evaluate the potential of a novel micro-CT system to image in vivo the extent of tumor in a rat model of malignant glioma compared to 3T magnetic resonance imaging (MRI) and histology. Fourteen animals underwent double dose contrast-enhanced imaging with micro-CT and 3T MRI using a clinical machine at day 10 after stereotactic F98 glioma cell implantation. Calculation of the volume of the contrast-uptaking part of the tumor was done by manually outlining the tumor contours by two experienced neuroradiologists. The micro-CT- and MRI-derived tumor volumes were compared to histology as gold standard (hematoxylin and eosin staining and fluorescence staining). There was high interobserver reability regarding the tumor volumes (Crombach's alpha>0.81). Also, there was good correlation of micro-CT- and high-field MRI-derived tumor volumes compared to histology: 72+/-21 mm3 and 69+/-23 mm3 compared to 81+/-14 mm3, respectively (r>0.76). Both the micro-CT- and MRI-derived tumor volumes were not significantly smaller compared to histology (P>0.14). In conclusion, micro-CT allows in vivo imaging of the contrast-enhancing part of experimental gliomas with an accuracy comparable to high-field MRI.
authors.affiliation: Department of Neuroradiology, University of Erlangen-Nuremberg, Schwabachanlage 6, D-91054 Erlangen, Germany. tobias.engelhorn@uk-erlangen.de
authors.forename: Tobias
authors.initials: T
authors.lastname: Engelhorn
authors.forename: Ilker Y
authors.initials: IY
authors.lastname: Eyupoglu
authors.forename: Marc A
authors.initials: MA
authors.lastname: Schwarz
authors.forename: Marek
authors.initials: M
authors.lastname: Karolczak
authors.forename: Holger
authors.initials: H
authors.lastname: Bruenner
authors.forename: Tobias
authors.initials: T
authors.lastname: Struffert
authors.forename: Willi
authors.initials: W
authors.lastname: Kalender
authors.forename: Arnd
authors.initials: A
authors.lastname: Doerfler
mesh_terms: D000818:Animals; D001932:Brain Neoplasms / Q000473:pathology* / Q000532:radiotherapy*; D003287:Contrast Media; D005260:Female; D005910:Glioma / Q000000981:diagnostic imaging* / Q000473:pathology*; D049452:Green Fluorescent Proteins / Q000235:genetics; D021621:Imaging, Three-Dimensional / Q000379:methods*; D008279:Magnetic Resonance Imaging / Q000379:methods; D009367:Neoplasm Staging; D051381:Rats; D011916:Rats, Inbred F344; D014057:Tomography, X-Ray Computed / Q000379:methods*; D014162:Transfection / Q000379:methods; D047368:Tumor Burden
chemical_list: D003287:Contrast Media; D049452:Green Fluorescent Proteins