title: Use of reverse transcriptase PCR to subtype N1 to N9 neuraminidase genes of avian influenza viruses.
abstract: Reverse transcriptase PCR designed to amplify the N1 to N9 neuraminidase (NA) genes of avian influenza viruses detected 118 of the 119 NA genes tested (99.2%) in a subtype-specific manner. This technique successfully subtyped all 167 recent avian influenza viruses isolated from birds. Subtype specificity was confirmed by sequence analyses of all 285 PCR products.
authors.affiliation: Research Team for Zoonotic Diseases, National Institute of Animal Health, Tsukuba, Ibaraki, Japan. ktsukamo@affrc.go.jp
authors.forename: Kenji
authors.initials: K
authors.lastname: Tsukamoto
authors.forename: Takayoshi
authors.initials: T
authors.lastname: Ashizawa
authors.forename: Koji
authors.initials: K
authors.lastname: Nakanishi
authors.forename: Noriyuki
authors.initials: N
authors.lastname: Kaji
authors.forename: Kotaro
authors.initials: K
authors.lastname: Suzuki
authors.forename: Makiko
authors.initials: M
authors.lastname: Shishido
authors.forename: Masatoshi
authors.initials: M
authors.lastname: Okamatsu
authors.forename: Masaji
authors.initials: M
authors.lastname: Mase
mesh_terms: D000818:Animals; D001717:Birds; D009980:Influenza A virus / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D005585:Influenza in Birds / Q000821:virology*; D008969:Molecular Sequence Data; D009439:Neuraminidase / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D017422:Sequence Analysis, DNA; D014764:Viral Proteins / Q000235:genetics*
chemical_list: D014764:Viral Proteins; C487630:NA protein, influenza A virus; D009439:Neuraminidase