Repeated intake of broccoli does not lead to higher plasma levels of sulforaphane in human volunteers.
The plasma pharmacokinetic characteristics of the chemopreventive isothiocyanate sulforaphane were determined in six human volunteers following single and repeated intake of raw broccoli. Initially, an analytical method utilising LC-MS/MS, capable of determining low levels of sulforaphane in human plasma was developed and validated. The plasma profile of the isothiocyanate best fitted a two-compartment pharmacokinetic model. Sulforaphane was rapidly absorbed with peak plasma levels being attained within 1.5h, and was characterised by a long terminal elimination phase. Repeated intake of broccoli had no impact on the pharmacokinetic behaviour or plasma levels of sulforaphane, and there was no evidence of accumulation.
Hanlon
Natalya
N
Molecular Toxicology Group, University of Surrey, Guildford, Surrey GU2 7XH, UK.
Coldham
Nick
N
Gielbert
Adriana
A
Sauer
Maurice J
MJ
Ioannides
Costas
C
D000293:Adolescent; D000328:Adult; D016588:Anticarcinogenic Agents / Q000097:blood*; D001937:Brassica / Q000737:chemistry*; D002853:Chromatography, Liquid; D005260:Female; D006801:Humans; D017879:Isothiocyanates; D008297:Male; D008875:Middle Aged; D013454:Sulfoxides; D053719:Tandem Mass Spectrometry; D013861:Thiocyanates / Q000097:blood*; D055815:Young Adult
D016588:Anticarcinogenic Agents; D017879:Isothiocyanates; D013454:Sulfoxides; D013861:Thiocyanates; C016766:sulforaphane