Endothelial nitric oxide synthase traffic inducer in the umbilical vessels of the patients with pre-eclampsia.
The expression of endothelial nitric oxide synthase traffic inducer (NOSTRIN) was examined in the umbilical vessels of the patients with pre-eclampsia (PE) to explore its possible role in the pathogenesis of PE. The NOSTRIN mRNA in umbilical tissues was determined by RT-PCR. The eNOS activity in umbilical vessels was spectrophotometrically detected. NO2-/NO3-, the stable metabolic end products of NO, was measured by using nitrate reductase. RT-PCR showed that the expression level of NOSTRIN was significantly higher in women with PE than in the normal group (P<0.01). The activity of eNOS was significantly decreased in PE group [(12.83+/-3.61) U/mg] than in normal group [(21.72+/-3.83) U/mg] (P<0.01). The level of NO2-/NO3- in PE patients (27.53+/-7.48) micromol/mg was significantly lower than that of normal group (54.27+/-9.53) micromol/mg (P<0.01). The significant negative correlation existed between the expression of NOSTRIN and the activity of eNOS in umbilical vessels of women with PE (r=-0.58, P<0.01). It was concluded that the level of NOSTRIN expression was increased in umbilical vessel of women with PE, indicating that it may be involved in the pathogenesis of PE.
Xiang
Wenpei
W
Center of Reproductive Medicine, Institute of Family Planning Research, Huazhong University of Science and Technology University, Wuhan, 430030, China. wen_pei_cn@sina.com
Chen
Hanping
H
Hu
Lian
L
Xu
Xiaoyan
X
D048868:Adaptor Proteins, Signal Transducing; D004268:DNA-Binding Proteins; D005260:Female; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics / Q000378:metabolism*; D011225:Pre-Eclampsia / Q000201:enzymology* / Q000209:etiology; D011247:Pregnancy; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D014469:Umbilical Arteries / Q000166:cytology / Q000201:enzymology*; D014471:Umbilical Veins / Q000166:cytology / Q000201:enzymology*
D048868:Adaptor Proteins, Signal Transducing; D004268:DNA-Binding Proteins; D047908:Intracellular Signaling Peptides and Proteins; C470345:NOSTRIN protein, human; D012333:RNA, Messenger