Application of human mini-satellite VNTR probes to genotyping in cynomolgus macaques.
Cynomolgus macaques (also known as longtail or crab-eating macaques) Macaca fascicularis, are an important non-human primate model for biomedical research. Using a case study involving a suspected occurrence of twin birth, we demonstrate that the highly polymorphic human variable-number tandem repeat (VNTR) mini-satellite probes D1S7, D2S44, D3S42 and D4S184 can be successfully employed as a rapid screening strategy to complement husbandry records in order to establish genetic relatedness in a captive colony.
Lavigne-Brunette
Lori
L
Health Canada, Health Products and Foods Branch, Biologics and Genetic Therapies Directorate, Centre for Biologics Research, Cellular and Molecular Biology Division, AL 2201E Sir FG Banting Research Laboratories, Ottawa, ON, Canada.
Joly
Marc-André
MA
Kelly
Susan
S
Frégeau
Chantal J
CJ
Aubin
Rémy A
RA
D000822:Animal Husbandry / Q000379:methods; D000818:Animals; D002616:Chemical Engineering / Q000379:methods; D004247:DNA / Q000097:blood / Q000235:genetics / Q000302:isolation & purification; D019143:Evolution, Molecular; D005260:Female; D005819:Genetic Markers / Q000235:genetics; D005838:Genotype; D006801:Humans; D007231:Infant, Newborn; D008168:Lung / Q000473:pathology; D008252:Macaca fascicularis / Q000235:genetics*; D008297:Male; D018598:Minisatellite Repeats / Q000235:genetics*; D050497:Stillbirth
D005819:Genetic Markers; D004247:DNA