title: Flux balance analysis: interrogating genome-scale metabolic networks.
abstract: Flux balance analysis (FBA) is a computational method to analyze reconstructions of biochemical networks. FBA requires the formulation of a biochemical network in a precise mathematical framework called a stoichiometric matrix. An objective function is defined (e.g., growth rate) toward which the system is assumed to be optimized. In this chapter, we present the methodology, theory, and common pitfalls of the application of FBA.
authors.affiliation: Department of Biomedical Engineering, University of Virginia, Charlottesville, USA.
authors.forename: Matthew A
authors.initials: MA
authors.lastname: Oberhardt
authors.forename: Arvind K
authors.initials: AK
authors.lastname: Chavali
authors.forename: Jason A
authors.initials: JA
authors.lastname: Papin
mesh_terms: D000465:Algorithms; D000818:Animals; D003198:Computer Simulation; D016678:Genome; D006801:Humans; D053858:Metabolic Networks and Pathways; D008954:Models, Biological; D012984:Software; D049490:Systems Biology / Q000379:methods*; D013816:Thermodynamics