title: Improvement of CXCR3 ligand CXCL11/I-TAC measurement in human plasma and serum.
abstract: The chemokine receptor CXCR3 is involved in cell trafficking dysregulation associated with several inflammatory conditions, including autoimmune and viral diseases. Downregulation of CXCR3, through binding with its ligand CXCL11 (I-TAC), represents a key mechanism in lymphocyte recruitment. Determination of circulating I-TAC can provide useful information in the investigation of inflammatory/infectious conditions. The existing commercial kit does not measure CXCL11/I-TAC in complex matrices, such as human plasma and serum, as reliably as in in vitro-generated cell culture supernatants. We here describe means which lead to an improvement of CXCL11/I-TAC measurement in human plasma and serum.
authors.affiliation: San Raffaele Scientific Institute, Lab. of Clinical Immunology, Via Stamira d'Ancona 20, 20127 Milan, Italy.
authors.forename: Viviana
authors.initials: V
authors.lastname: Cremasco
authors.forename: Barbara
authors.initials: B
authors.lastname: Mantelli
authors.forename: Adriano
authors.initials: A
authors.lastname: Lazzarin
authors.forename: Priscilla
authors.initials: P
authors.lastname: Biswas
mesh_terms: D000925:Anticoagulants; D054371:Chemokine CXCL11 / Q000097:blood* / Q000378:metabolism; D004492:Edetic Acid; D004797:Enzyme-Linked Immunosorbent Assay; D006493:Heparin; D006801:Humans; D008024:Ligands; D011933:Reagent Kits, Diagnostic; D054367:Receptors, CXCR3 / Q000378:metabolism*
chemical_list: D000925:Anticoagulants; D054371:Chemokine CXCL11; D008024:Ligands; D011933:Reagent Kits, Diagnostic; D054367:Receptors, CXCR3; D006493:Heparin; D004492:Edetic Acid