title: Immunoproteomics reveals that cancer of the tongue and the gingivobuccal complex exhibit differential autoantibody response.
abstract: Autoantibody response to tumor antigens has been widely used to identify novel tumor markers for different cancers, including that of the head and neck. The oral cavity, which is in the head and neck region, comprises of many sub sites with distinct biologies and incidence of cancer of each sub site of the oral cavity is different. It is anticipated therefore that each sub site of the oral cavity may elicit a differential autoantibody response. This report evaluates the autoantibody response in 15 patients with cancer of gingivo-buccal complex and in 15 patients with cancer of tongue using Immunoproteomics, and shows that the autoantibody response to alpha-enolase, HSP 70, peroxiredoxin-VI, annexin II, pyruvate kinase, alpha-tubulin, beta-tubulin, ATP synthase, triose phosphate isomerase and aldose reductase seen in patients with cancer of gingivo-buccal complex is absent in patients with cancer of tongue. This suggests that cancer of these sub sites should be studied separately because of their different biology and emerging site specific molecular signatures including autoantibody responses to ensure unambiguous clinical interpretations.
authors.affiliation: Advanced Centre for Treatment, Research and Education in Cancer (ACTREC), Tata Memorial Centre, Kharghar, Navi Mumbai, India.
authors.forename: Sanjeev
authors.initials: S
authors.lastname: Shukla
authors.forename: Atul
authors.initials: A
authors.lastname: Pranay
authors.forename: Anil K
authors.initials: AK
authors.lastname: D'Cruz
authors.forename: Pankaj
authors.initials: P
authors.lastname: Chaturvedi
authors.forename: Shubhada V
authors.initials: SV
authors.lastname: Kane
authors.forename: Surekha M
authors.initials: SM
authors.lastname: Zingde
mesh_terms: D000328:Adult; D000368:Aged; D000449:Aldehyde Reductase / Q000276:immunology; D000595:Amino Acid Sequence; D017306:Annexin A2 / Q000276:immunology; D001323:Autoantibodies / Q000032:analysis* / Q000097:blood; D001798:Blood Proteins / Q000032:analysis* / Q000276:immunology; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D005887:Gingival Neoplasms / Q000097:blood / Q000276:immunology*; D018840:HSP70 Heat-Shock Proteins / Q000276:immunology; D006801:Humans; D008297:Male; D013058:Mass Spectrometry; D008875:Middle Aged; D008969:Molecular Sequence Data; D009062:Mouth Neoplasms / Q000097:blood / Q000276:immunology* / Q000473:pathology; D054465:Peroxiredoxin VI / Q000276:immunology; D010751:Phosphopyruvate Hydratase; D040901:Proteomics / Q000379:methods*; D006180:Proton-Translocating ATPases / Q000276:immunology; D011770:Pyruvate Kinase / Q000276:immunology; D014305:Triose-Phosphate Isomerase / Q000276:immunology; D014404:Tubulin / Q000276:immunology
chemical_list: D017306:Annexin A2; D001323:Autoantibodies; D001798:Blood Proteins; D018840:HSP70 Heat-Shock Proteins; D014404:Tubulin; D000449:Aldehyde Reductase; D054465:Peroxiredoxin VI; D011770:Pyruvate Kinase; D006180:Proton-Translocating ATPases; D010751:Phosphopyruvate Hydratase; D014305:Triose-Phosphate Isomerase