Expression and hypoxic up-regulation of neuroglobin in human glioblastoma cells.
Neuroglobin is a recently identified globin molecule that is expressed predominantly in the vertebrate brain. Neuroglobin expression increases in oxygen-deprived neurons, suggesting it protects neurons from ischemic cell death. We report that neuroglobin transcript and protein are expressed in human glioblastoma cells, and that this expression increases in hypoxia in vitro. We also show that neuroglobin is up-regulated in hypoxic microregions of glioblastoma tumor xenografts. Our finding of hypoxic up-regulation of neuroglobin in human glioblastoma cells may provide insight into how tumor cells adapt to and survive in hypoxic microenvironments.
Emara
Marwan
M
Department of Oncology, University of Alberta, Cross Cancer Institute, 11560 University Avenue, Edmonton, Alberta, T6G 1Z2, Canada.
Salloum
Nicole
N
Allalunis-Turner
Joan
J
D000818:Animals; D045744:Cell Line, Tumor; D015972:Gene Expression Regulation, Neoplastic; D005909:Glioblastoma / Q000235:genetics / Q000473:pathology*; D005914:Globins / Q000235:genetics*; D006801:Humans; D000860:Hypoxia / Q000235:genetics*; D051379:Mice; D009374:Neoplasms, Experimental; D009419:Nerve Tissue Proteins / Q000235:genetics*; D000078787:Neuroglobin; D014183:Transplantation, Heterologous; D015854:Up-Regulation / Q000235:genetics
D009419:Nerve Tissue Proteins; D000078787:Neuroglobin; D005914:Globins