title: Expression of OATP1B3 determines uptake of Gd-EOB-DTPA in hepatocellular carcinoma.
abstract: BACKGROUND
  Gadolinium-ethoxybenzyl-diethylenetriamine pentaacetic acid (Gd-EOB-DTPA) is an MRI contrast agent with perfusion and hepatoselective properties. The purpose of the study was to examine uptake of Gd-EOB-DTPA in the hepatobiliary phase in hepatocellular carcinoma (HCC).
  
  
  METHODS
  A retrospective analysis of 22 patients with HCC who underwent preoperative Gd-EOB-DTPA-enhanced MRI was performed. Enhancement ratios (ERs) and expression levels of the organic anion transporter (OATP) 1B3 protein were examined.
  
  
  RESULTS
  Gd-EOB-DTPA accumulated in the hepatobiliary phase in 6 of the 22 cases. All 6 Gd-EOB-DTPA-positive cases were moderately differentiated HCC, but 11 other moderately differentiated HCCs did not show Gd-EOB-DTPA uptake. Histopathologically, 4 Gd-EOB-DTPA-positive HCCs and 5 Gd-EOB-DTPA-negative HCCs produced bile. HCCs with Gd-EOB-DTPA uptake overexpressed OATP1B3 compared with HCCs without Gd-EOB-DTPA uptake, and OATP1B3 levels were significantly correlated with ERs (r=0.91, P<0.0001).
  
  
  CONCLUSIONS
  Uptake of Gd-EOB-DTPA in HCC is determined by expression of OATP1B3 rather than by tumor differentiation or bile production.
authors.affiliation: Department of Surgery, Graduate School of Medicine, Kyoto University, and Department of Diagnostic Pathology, Kyoto University Hospital, 54 Shogoin-Kawahara-cho, Sakyo-ku, Kyoto, 606-8507, Japan.
authors.forename: Masato
authors.initials: M
authors.lastname: Narita
authors.forename: Etsuro
authors.initials: E
authors.lastname: Hatano
authors.forename: Shigeki
authors.initials: S
authors.lastname: Arizono
authors.forename: Aya
authors.initials: A
authors.lastname: Miyagawa-Hayashino
authors.forename: Hiroyoshi
authors.initials: H
authors.lastname: Isoda
authors.forename: Koji
authors.initials: K
authors.lastname: Kitamura
authors.forename: Kojiro
authors.initials: K
authors.lastname: Taura
authors.forename: Kentaro
authors.initials: K
authors.lastname: Yasuchika
authors.forename: Takashi
authors.initials: T
authors.lastname: Nitta
authors.forename: Iwao
authors.initials: I
authors.lastname: Ikai
authors.forename: Shinji
authors.initials: S
authors.lastname: Uemoto
mesh_terms: D001646:Bile / Q000378:metabolism; D006528:Carcinoma, Hepatocellular / Q000175:diagnosis* / Q000378:metabolism*; D003287:Contrast Media / Q000378:metabolism; D005260:Female; D019786:Gadolinium DTPA / Q000378:metabolism*; D006801:Humans; D008113:Liver Neoplasms / Q000175:diagnosis* / Q000378:metabolism*; D008279:Magnetic Resonance Imaging / Q000379:methods*; D008297:Male; D008875:Middle Aged; D029362:Organic Anion Transporters, Sodium-Independent / Q000378:metabolism*; D012189:Retrospective Studies; D000074542:Solute Carrier Organic Anion Transporter Family Member 1B3
chemical_list: D003287:Contrast Media; D029362:Organic Anion Transporters, Sodium-Independent; C421209:SLCO1B3 protein, human; D000074542:Solute Carrier Organic Anion Transporter Family Member 1B3; C073590:gadolinium ethoxybenzyl DTPA; D019786:Gadolinium DTPA