Characterization of signals that dictate nuclear/nucleolar and cytoplasmic shuttling of the capsid protein of Tomato leaf curl Java virus associated with DNA beta satellite.
Transport of the viral genome into the nucleus is an obligatory step in the replication cycle of geminiviruses. Capsid proteins (CPs) of geminiviruses are multifunctional proteins thought to be involved in this process. The CP of monopartite geminiviruses is absolutely essential for virus movement. To more precisely examine the role of CP, we have constructed a series of single and double deletions into the coding sequence of Tomato leaf curl Java virus (ToLCJAV) CP and examined sub-cellular localization using transient expression of GFP fusion proteins. In this report, the domains of the CP encoded by ToLCJAV localized in the nucleus/nucleolus and cytoplasm in transfected cells were mapped. Deletion analysis revealed that the Arg-rich cluster from amino acids (aa) (16)KVRRR(20) in the N-terminal region of CP functioned as nuclear/nucleolar localization signals (NLSs). The region from aa (52)RKPR(55) contained basic amino acid cluster was capable to redirect the CP to the nucleus. Further, both transient expression and yeast hybrid assays demonstrated that CP was capable of shuttling between the nucleus and cytoplasm of the cell. Deletion mutant analysis revealed that this property was attributed to a nuclear export signal (NES) sequence consisted of aa ((245)LKIRIY(250)) reside at C-terminal part of CP. This hydrophobic region caused transport of GFP to the cytoplasm. However, ToLCJAV CP NLSs and NES show peculiarities in the number and position of basic residues. Taken together, these results demonstrated that ToLCJAV CP shuttles between the nucleus and cytoplasm, such an activity homolog to bipartite geminivirus BV1 ORF.
Sharma
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P
Department of Life Science, Graduate School of Agricultural Science, Tohoku University, 1-1 Tsutsumidori - Amamiyamachi, Aoba-ku, Sendai 981-8555, Japan. neprads@yahoo.com
Ikegami
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M
D053839:Begomovirus / Q000235:genetics / Q000502:physiology*; D036022:Capsid Proteins / Q000235:genetics* / Q000378:metabolism*; D002466:Cell Nucleolus / Q000737:chemistry*; D002467:Cell Nucleus / Q000737:chemistry*; D004252:DNA Mutational Analysis; D017930:Genes, Reporter; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D018551:Solanum lycopersicum; D021382:Protein Sorting Signals; D017384:Sequence Deletion; D014779:Virus Replication
D036022:Capsid Proteins; D021382:Protein Sorting Signals; D049452:Green Fluorescent Proteins