title: Fine mapping of quantitative trait loci for mastitis resistance on bovine chromosome 11.
abstract: Quantitative trait loci (QTL) affecting clinical mastitis (CM) and somatic cell score (SCS) were mapped on bovine chromosome 11. The mapping population consisted of 14 grandsire families belonging to three Nordic red cattle breeds: Finnish Ayrshire (FA), Swedish Red and White (SRB) and Danish Red. The families had previously been shown to segregate for udder health QTL. A total of 524 progeny tested bulls were included in the analysis. A linkage map including 33 microsatellite and five SNP markers was constructed. We performed combined linkage disequilibrium and linkage analysis (LDLA) using the whole data set. Further analyses were performed for FA and SRB separately to study the origin of the identified QTL/haplotype and to examine if it was common in both populations. Finally, different two-trait models were fitted. These postulated either a pleiotropic QTL affecting both traits; two linked QTL, each affecting one trait; or one QTL affecting a single trait. A QTL affecting CM was fine-mapped. In FA, a haplotype having a strong association with a high negative effect on mastitis resistance was identified. The mapping precision of an earlier detected SCS-QTL was not improved by the LDLA analysis because of lack of linkage disequilibrium between the markers used and the QTL in the region.
authors.affiliation: MTT, Agrifood Research Finland, Biotechnology and Food Research, FIN-31600 Jokioinen, Finland. nina.schulman@mtt.fi
authors.forename: N F
authors.initials: NF
authors.lastname: Schulman
authors.forename: G
authors.initials: G
authors.lastname: Sahana
authors.forename: T
authors.initials: T
authors.lastname: Iso-Touru
authors.forename: M S
authors.initials: MS
authors.lastname: Lund
authors.forename: L
authors.initials: L
authors.lastname: Andersson-Eklund
authors.forename: S M
authors.initials: SM
authors.lastname: Viitala
authors.forename: S
authors.initials: S
authors.lastname: Värv
authors.forename: H
authors.initials: H
authors.lastname: Viinalass
authors.forename: J H
authors.initials: JH
authors.lastname: Vilkki
mesh_terms: D000818:Animals; D002417:Cattle / Q000235:genetics*; D002418:Cattle Diseases / Q000235:genetics*; D002874:Chromosome Mapping; D033481:Chromosomes, Mammalian; D005260:Female; D007113:Immunity, Innate / Q000235:genetics*; D008413:Mastitis / Q000235:genetics / Q000662:veterinary*; D020641:Polymorphism, Single Nucleotide; D040641:Quantitative Trait Loci