Proteomic and bioinformatic analysis on endocrine organs of domesticated silkworm, Bombyx mori L. for a comprehensive understanding of their roles and relations.
Three organs of silkworm larva endocrine system, including brain (Br), subesophageal ganglion (SG) and prothoracic glands (PG), were studied employing shotgun LC-MS/MS combined with bioinformatic analysis to comprehensively understand their roles and relations. Totally, 3430, 2683, and 3395 proteins were identified including 1885 common and 652, 253, and 790 organ-specific ones in Br, SG, and PG, respectively. Identified common-expressed proteins indicated the existence of intrinsic complex interactions among these parts of endocrine system. Most of the reputed organs-specific proteins were identified by this approach. KEGG pathway analysis showed 162 same pathways among the 169, 164, and 171 relating Br, SG, and PG. This analysis revealed functional similarities with exceptional resemblance in their metabolism and signaling pathways of the three organs. On the other hand, 70, 57, and 114 organ-specific enzymes related pathways were detected for Br, SG, and PG confirming their functional differences. These results reveal a cooperative mechanism among the three endocrine organs in regulating various physiological and developmental events, and also suggest that the organ-specific proteins might be the fundamental factors responsible for the functional differentiation of these organs.
Li
Jian-Ying
JY
College of Animal Sciences, Zhejiang University, Hangzhou 310029, P. R. China.
Chen
Xiang
X
Fan
Wei
W
Moghaddam
S Hossein Hosseini
SH
Chen
Ming
M
Zhou
Zhong-Hua
ZH
Yang
Hui-Juan
HJ
Chen
Jin-E
JE
Zhong
Bo-Xiong
BX
D000818:Animals; D012831:Bombyx / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D002853:Chromatography, Liquid; D019295:Computational Biology; D004702:Endocrine Glands / Q000737:chemistry / Q000378:metabolism; D015870:Gene Expression; D019476:Insect Proteins / Q000235:genetics / Q000378:metabolism*; D007328:Insulin / Q000378:metabolism; D008675:Metamorphosis, Biological; D008954:Models, Biological; D020543:Proteome / Q000378:metabolism*; D040901:Proteomics; D015203:Reproducibility of Results; D015398:Signal Transduction; D053719:Tandem Mass Spectrometry
D019476:Insect Proteins; D007328:Insulin; D020543:Proteome