title: A review of non-invasive optical-based image analysis systems for continuous bioprocess monitoring.
abstract: To observe and control cultivation processes, optical sensors are used increasingly. Important variables for controlling such processes are cell count, cell size distribution and the morphology of cells. Among turbidity measurement methods, imaging procedures are applied for determining these process values. A disadvantage of most previously developed imaging procedures is that they are only available offline, which requires sampling. On the other hand, available imaging inline probes can only deliver a limited number of process values so far. This contribution gives an overview of optical procedures for the inline determination of cell count, cell size distribution and other variables. In particular, by in situ microscopy, an imaging procedure will be described, which allows the determination of direct and non-direct cell variables in real time without sampling.
authors.affiliation: Institut für Technische Chemie, Gottfried Wilhelm Leibniz Universität Hannover, Hannover, Germany. hoepfner@iftc.uni-hannover.de
authors.forename: Tim
authors.initials: T
authors.lastname: Höpfner
authors.forename: Arne
authors.initials: A
authors.lastname: Bluma
authors.forename: Guido
authors.initials: G
authors.lastname: Rudolph
authors.forename: Patrick
authors.initials: P
authors.lastname: Lindner
authors.forename: Thomas
authors.initials: T
authors.lastname: Scheper
mesh_terms: D000465:Algorithms; D000818:Animals; D001709:Biotechnology / Q000295:instrumentation* / Q000379:methods*; D006224:Cricetinae; D003460:Crystallization; D004867:Equipment Design; D005055:Euglena / Q000378:metabolism; D006801:Humans; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D008853:Microscopy / Q000379:methods; D008856:Microscopy, Fluorescence / Q000379:methods; D009391:Nephelometry and Turbidimetry / Q000379:methods; D055095:Optics and Photonics; D010316:Particle Size; D010843:Pichia / Q000378:metabolism; D013997:Time Factors