Quantitative SERRS immunoassay for the detection of human PSA.
We report the first use of a commonly used ELISA colorimetric substrate as a SERRS marker and show how it can be used for the detection of pg ml(-1) levels of human prostate specific antigen (PSA) in clinical samples. The technique is amenable over a wide range of concentrations and lends itself to future multiplexing analysis.
Stevenson
Ross
R
Centre for Molecular Nanometrology, WestCHEM, Department of Pure and Applied Chemistry, University of Strathclyde, Glasgow, UK G1 1XL.
Ingram
Andrew
A
Leung
Hing
H
McMillan
Donald C
DC
Graham
Duncan
D
D052160:Benzothiazoles; D003124:Colorimetry; D004797:Enzyme-Linked Immunosorbent Assay / Q000295:instrumentation / Q000379:methods*; D006801:Humans; D008297:Male; D017430:Prostate-Specific Antigen / Q000097:blood*; D013059:Spectrum Analysis, Raman / Q000295:instrumentation / Q000379:methods*; D013451:Sulfonic Acids; D013844:Thiazoles; D013997:Time Factors
D052160:Benzothiazoles; D013451:Sulfonic Acids; D013844:Thiazoles; C002502:2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid; D017430:Prostate-Specific Antigen