Pyrosequencing analysis of 20 nucleotides of internal transcribed spacer 2 discriminates Candida parapsilosis, Candida metapsilosis, and Candida orthopsilosis.
Two new cryptic sister species, Candida orthopsilosis and Candida metapsilosis, were recently identified by consistent DNA sequence differences among several genes within the genetically heterogeneous Candida parapsilosis complex. Here, we present data demonstrating that Pyrosequencing analysis of 20 nucleotides of internal transcribed spacer region 2 rapidly and robustly distinguishes between these three closely related Candida species.
Borman
Andrew M
AM
Mycology Reference Laboratory, Health Protection Agency, Bristol, United Kingdom. Andy.Borman@uhBristol.nhs.uk
Linton
Christopher J
CJ
Oliver
Debra
D
Palmer
Michael D
MD
Szekely
Adrien
A
Odds
Frank C
FC
Johnson
Elizabeth M
EM
D002175:Candida / Q000145:classification* / Q000235:genetics*; D021903:DNA, Ribosomal Spacer / Q000737:chemistry* / Q000235:genetics*; D006801:Humans; D012680:Sensitivity and Specificity; D017422:Sequence Analysis, DNA / Q000379:methods*
D021903:DNA, Ribosomal Spacer