The secreted Helicobacter cysteine-rich protein A causes adherence of human monocytes and differentiation into a macrophage-like phenotype.
Helicobacter pylori genomes typically contain 8 or 9 genes that code for secreted and highly disulfide-bridged proteins designated Helicobacter cysteine-rich proteins (Hcp). Here we show that HcpA (hp0211) but not HcpC (hp1098) triggers the differentiation of human myeloid Thp1 monocytes into macrophages. Small amounts of HcpA cause the transition of round-shaped monocytes into cells with star-like morphologies, adherence to the culture dish surface, phagocytosis of opsonized fluorescent microspheres, and expression of the surface marker protein CD11b, all of which are indicative of a macrophage-like phenotype. We conclude that HcpA acts as a bacterial immune modulator similar to a eukaryotic cytokine.
Dumrese
Claudia
C
Center for Microscopy and Image Analysis, University of Zürich, Winterthurer Strasse 190, Zürich, Switzerland.
Slomianka
Lutz
L
Ziegler
Urs
U
Choi
Sung Sook
SS
Kalia
Awdhesh
A
Fulurija
Alma
A
Lu
Wei
W
Berg
Douglas E
DE
Benghezal
Mohammed
M
Marshall
Barry
B
Mittl
Peer R E
PR
D001426:Bacterial Proteins / Q000378:metabolism*; D002448:Cell Adhesion; D002454:Cell Differentiation; D016480:Helicobacter pylori / Q000378:metabolism*; D006801:Humans; D008264:Macrophages / Q000166:cytology* / Q000378:metabolism; D008855:Microscopy, Electron, Scanning; D009000:Monocytes / Q000166:cytology* / Q000378:metabolism; D010587:Phagocytosis; D010641:Phenotype; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D001618:beta-Lactamases / Q000378:metabolism*
D001426:Bacterial Proteins; D011994:Recombinant Proteins; C410728:hcpA protein, Helicobacter pylori; D001618:beta-Lactamases