[Biocompatibility between human adipose-derived mesenchymal stem cells and porcine bone scaffolds].
OBJECTIVE
To study the compatibility between human adipose-derived mesenchymal stem cells and porcine bone scaffolds.


METHODS
Porcine bone tissues were co-cultured with adipose-derived mesenchymal stem cells, and the complex was observed under scanning electron microscope. The viability and alkaline phosphatase (ALP) activity of the cells were examined with the cells co-cultured with human bone scaffold as the control.


RESULTS
At 4 and 10 days after the co-culture, the adipose-derived mesenchymal stem cells were observed to extend pseudopodia to adhere to the two scaffold materials. MTT assay showed that the cell proliferation on both of the materials increased with time, and the two cell complexes exhibited similar pattern of changes in ALP activity.


CONCLUSIONS
As the seed cells, human adipose-derived mesenchymal stem cells exhibit good comparability with porcine bone scaffold, suggesting their potential of constructing tissue-engineered bone graft.
Zhang
Xue-quan
XQ
Department of Human Anatomy, South Medical University, Guangzhou 510510, China. jpzxq@126.com
Yuag
Lin
L
Yang
Lin-lin
LL
Jang
Xue-mei
XM
Yang
Chun
C
Yu
Lei
L
Dai
Jing-xing
JX
D000273:Adipose Tissue / Q000166:cytology*; D000328:Adult; D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D001842:Bone and Bones / Q000166:cytology* / Q000378:metabolism; D049109:Cell Proliferation; D018920:Coculture Techniques; D006801:Humans; D008422:Materials Testing; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000201:enzymology / Q000378:metabolism / Q000648:ultrastructure; D008855:Microscopy, Electron, Scanning; D013552:Swine; D013997:Time Factors; D054457:Tissue Scaffolds
D000469:Alkaline Phosphatase