title: [Study on preparation and quantity determination of Scrophularia ningpoensis dispensing granules].
abstract: OBJECTIVE
  To develop the preparation method and to determine the quantity of effective constituents of Scrophularia ningpoensis dispensing granules.
  
  
  METHODS
  Nine experiments were carried out through L9 (3(4)) orthogonal design. The contents of harpagoside and cinnamic acid were determined by HPLC method.
  
  
  RESULTS
  The optimal extraction process was as follows: the slices were soaked in water in the ratio of 1:8 for 0.5 h, then they were decocted for 2 h. For the second time, 6 times of water was added and sustained for 1.5 h. Harpagoside and cinnamic acid were linear within the ranges of 0.0776-1.552 microg (r=0.9999) and 0.01446-0.4339 microg (r=0.9999), respectively. The average recoveries were 100.4% (RSD 1.98%, n=6) and 98.27% (RSD1.35%, n=6), respectively. Ten batches of granules were determined.
  
  
  CONCLUSIONS
  The extraction process is scientific and the determination method is suitable for quality control of Scrophularia ningpoensis dispensing granules.
authors.affiliation: Division of Pharmacognosy, School of Pharmaceutical Sciences, Peking University, Beijing 100083, China.
authors.forename: Zhengrui
authors.initials: Z
authors.lastname: Niu
authors.forename: Rufeng
authors.initials: R
authors.lastname: Wang
authors.forename: Yu
authors.initials: Y
authors.lastname: Jing
authors.forename: Mingying
authors.initials: M
authors.lastname: Shang
authors.forename: Shaoqing
authors.initials: S
authors.lastname: Cai
mesh_terms: D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D002934:Cinnamates / Q000032:analysis; D004339:Drug Compounding / Q000379:methods* / Q000592:standards; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage* / Q000737:chemistry*; D006027:Glycosides / Q000032:analysis; D016014:Linear Models; D011714:Pyrans / Q000032:analysis; D011786:Quality Control; D015203:Reproducibility of Results; D032266:Scrophularia / Q000737:chemistry*; D012680:Sensitivity and Specificity; D012997:Solvents / Q000737:chemistry; D013696:Temperature
chemical_list: D002934:Cinnamates; D004365:Drugs, Chinese Herbal; D006027:Glycosides; D011714:Pyrans; D012997:Solvents; C029010:cinnamic acid; C033249:harpagoside