title: FOXP3 defines regulatory T cells in human tumor and autoimmune disease.
abstract: Activated T cells may express FOXP3. It is thought that FOXP3 is not a specific marker to determine regulatory T cells (Treg) in humans. Here, we examined the functional phenotype and cytokine profile of the in vitro induced FOXP3(+) T cells, primary FOXP3(+) and FOXP3(-) T cells in patients with ulcerative colitis and tumors including colon carcinoma, melanoma, hepatic carcinoma, ovarian carcinoma, pancreatic cancer, and renal cell carcinoma. We observed similar levels of suppressive capacity of primary FOXP3(+) T cells in blood, tumors, and colitic tissues. Compared with primary FOXP3(-) T cells in the same microenvironment, these primary FOXP3(+) T cells expressed minimal levels of effector cytokines, negligible amount of cytotoxic molecule granzyme B, and levels of suppressive molecules interleukin-10 and PD-1. Although the in vitro activated T cells expressed FOXP3, these induced FOXP3(+) T cells expressed high levels of multiple effector cytokines and were not functionally suppressive. The data reinforce the fact that FOXP3 remains an accurate marker to define primary Tregs in patients with cancer and autoimmune disease. We suggest that the combination of FOXP3 and cytokine profile is useful for further functionally distinguishing primary Tregs from activated conventional T cells.
authors.affiliation: Department of Surgery, University of Michigan, Ann Arbor, Michigan 48109, USA.
authors.forename: Ilona
authors.initials: I
authors.lastname: Kryczek
authors.forename: Rebecca
authors.initials: R
authors.lastname: Liu
authors.forename: Guobin
authors.initials: G
authors.lastname: Wang
authors.forename: Ke
authors.initials: K
authors.lastname: Wu
authors.forename: Xiaogong
authors.initials: X
authors.lastname: Shu
authors.forename: Wojciech
authors.initials: W
authors.lastname: Szeliga
authors.forename: Linhua
authors.initials: L
authors.lastname: Vatan
authors.forename: Emily
authors.initials: E
authors.lastname: Finlayson
authors.forename: Emina
authors.initials: E
authors.lastname: Huang
authors.forename: Diane
authors.initials: D
authors.lastname: Simeone
authors.forename: Bruce
authors.initials: B
authors.lastname: Redman
authors.forename: Theodore H
authors.initials: TH
authors.lastname: Welling
authors.forename: Alfred
authors.initials: A
authors.lastname: Chang
authors.forename: Weiping
authors.initials: W
authors.lastname: Zou
mesh_terms: D003093:Colitis, Ulcerative / Q000097:blood / Q000276:immunology*; D016207:Cytokines / Q000276:immunology; D005434:Flow Cytometry; D051858:Forkhead Transcription Factors / Q000097:blood / Q000276:immunology*; D006801:Humans; D009369:Neoplasms / Q000097:blood / Q000276:immunology*; D050378:T-Lymphocytes, Regulatory / Q000276:immunology*
chemical_list: D016207:Cytokines; C418974:FOXP3 protein, human; D051858:Forkhead Transcription Factors