Amphiphilic random glycopolymer based on phenylboronic acid: synthesis, characterization, and potential as glucose-sensitive matrix.
This study is devoted to developing amphiphilic, random glycopolymers based on phenylboronic acid, which self-assemble to form nanoparticles (NPs), as a glucose-sensitive agent. Maleimide-glucosamine was copolymerized with 3-acryl aminophenylboronic acid in methanol at 70 degrees C. Using the nanoprecipitation method, NPs with a narrow size distribution were successfully generated. Transmission electron microscopic analysis showed that the NPs were well dispersed as individual, spherically shaped particles. The swelling behavior of the NPs and the in vitro release profiles of insulin at different glucose concentrations revealed definite glucose sensitivity of the glycopolymers. Further, circular dichroism spectroscopy demonstrated that the overall tertiary structure of the released insulin was not altered compared with standard insulin. The analysis of relative cell proliferation suggested that the glycopolymer NPs had good biocompatibility. The glycopolymers that responded to changes in the glucose concentration of the surrounding environment are being aimed for use in self-regulated insulin delivery.
Jin
Xingju
X
Key Laboratory of Functional Polymer Materials of Ministry Education, Institute of Polymer Chemistry, Nankai University, Tianjin, China.
Zhang
Xinge
X
Wu
Zhongming
Z
Teng
Dayong
D
Zhang
Xuejiao
X
Wang
Yanxia
Y
Wang
Zhen
Z
Li
Chaoxing
C
D001672:Biocompatible Materials; D001897:Boronic Acids / Q000737:chemistry*; D002942:Circular Dichroism; D005947:Glucose / Q000737:chemistry*; D007328:Insulin / Q000008:administration & dosage; D009682:Magnetic Resonance Spectroscopy; D046529:Microscopy, Electron, Transmission; D011108:Polymers / Q000138:chemical synthesis* / Q000737:chemistry
D001672:Biocompatible Materials; D001897:Boronic Acids; D007328:Insulin; D011108:Polymers; D005947:Glucose; C010686:benzeneboronic acid