title: SELDI-TOF MS detection of urinary hepcidin.
abstract: Hepcidin is a 25-residue hepatic peptide that regulates iron absorption from the diet and tissue iron distribution. Inappropriately low Hepcidin expression is implicated in the pathogenesis of hereditary hemochromatosis and iron-loading anemias, like the thalassemias. Increased hepcidin expression mediates iron retention in the anemias of inflammation and plays a pathogenic role in iron-refractory iron-deficiency anemia (IRIDA). Because of its clinical importance, Hepcidin is expected to be a useful biomarker for diagnosis and management of iron-related disorders. So far an ELISA for human hepcidin and SELDI-TOF-MS based approaches have been applied to monitor urinary and/or serum hepcidin levels. Here we report a modified protocol for SELDI-TOF based detection of human, urinary hepcidin. We show that CM10 Proteinchips are superior to NP20 Proteinchips commonly used in previously reported protocols to sensitively and accurately detect urinary hepcidin. Application of this modified hepcidin assay accurately detects increased hepcidin levels in the urine of sepsis patients.
authors.affiliation: Department of Pediatric Oncology, Haematology and Immunology, University Hospital of Heidelberg; Molecular Medicine Partnership Unit, Im Neuenheimer Feld 156, 69120 Heidelberg, Germany.
authors.forename: Sandro
authors.initials: S
authors.lastname: Altamura
authors.forename: Judit
authors.initials: J
authors.lastname: Kiss
authors.forename: Claudia
authors.initials: C
authors.lastname: Blattmann
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Gilles
authors.forename: Martina U
authors.initials: MU
authors.lastname: Muckenthaler
mesh_terms: D023181:Antimicrobial Cationic Peptides / Q000652:urine*; D005260:Female; D064451:Hepcidins; D006801:Humans; D008297:Male; D015203:Reproducibility of Results; D018805:Sepsis / Q000652:urine; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
chemical_list: D023181:Antimicrobial Cationic Peptides; C578702:HAMP protein, human; D064451:Hepcidins