Brevibacillus panacihumi sp. nov., a beta-glucosidase-producing bacterium.
Two Gram-positive-staining, endospore-forming, rod-shaped, motile bacteria, strains DCY35(T) and C17, were isolated from soil of a ginseng field in South Korea and were characterized in order to determine their taxonomic positions. 16S rRNA gene sequence analysis revealed that the two strains belonged to the family Paenibacillaceae; strain DCY35(T) showed highest levels of similarity to strain C17 (99.9 %), Brevibacillus invocatus LMG 18962(T) (98.9 %), B. centrosporus DSM 8445(T) (98.0 %), B. borstelensis DSM 6347(T) (97.6 %), B. formosus DSM 9885(T) (97.4 %), B. agri DSM 6348(T) (97.3 %), B. brevis DSM 30(T) (97.3 %) and B. levickii LMG 22481(T) (97.0 %). Chemotaxonomic analyses revealed that strains DCY35(T) and C17 possess menaquinone MK-7, common to members of the genus Brevibacillus, and that the predominant fatty acids were iso-C(15 : 0) (37.3 % of the total), anteiso-C(15 : 0) (32.9 %), iso-C(14 : 0) (11.8 %) and iso-C(16 : 0) (6.5 %). The results of physiological and biochemical tests clearly demonstrated that strains DCY35(T) and C17 represent a distinct species. Based on these data, the two strains are considered to represent a novel species of the genus Brevibacillus, for which the name Brevibacillus panacihumi sp. nov. is proposed. The type strain is DCY35(T) (=KCTC 13206(T) =JCM 15085(T)).
Kim
Myung Kyum
MK
Korean Ginseng Center and Ginseng Genetic Resource Bank, Kyung Hee University, 1 Seocheon-dong, Giheung-gu Yongin-si, Gyeonggi-do 449-701, Republic of Korea.
Sathiyaraj
Srinivasan
S
Pulla
Rama Krishna
RK
Yang
Deok-Chun
DC
D015373:Bacterial Typing Techniques; D004269:DNA, Bacterial / Q000032:analysis / Q000302:isolation & purification; D004275:DNA, Ribosomal / Q000032:analysis; D005227:Fatty Acids / Q000032:analysis; D005838:Genotype; D016937:Gram-Positive Endospore-Forming Rods / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology; D007723:Korea; D008969:Molecular Sequence Data; D005894:Panax; D010641:Phenotype; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D013045:Species Specificity; D001617:beta-Glucosidase / Q000096:biosynthesis*
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D001617:beta-Glucosidase