An ultrasensitive rapid immunocytotoxicity assay for detecting Clostridium difficile toxins.
We describe a novel ultrasensitive cell-based immunocytotoxicity assay for detecting Clostridium difficile toxin A and B. The assay is simple to perform with a turnaround time of approximately 3 h . It is particularly sensitive in detecting TcdA at a level less then 1 pg/ml. Using this assay, we were able to detect the presence of C. difficile toxins in the fecal and serum specimens of experimentally infected piglets.
He
Xiangyun
X
Division of Infectious Diseases, Department of Biomedical Sciences, Tufts University Cummings School of Veterinary Medicine, North Grafton, Massachusetts 01536, USA.
Wang
Jufang
J
Steele
Jennifer
J
Sun
Xingmin
X
Nie
Weijia
W
Tzipori
Saul
S
Feng
Hanping
H
D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology; D001427:Bacterial Toxins / Q000276:immunology / Q000302:isolation & purification* / Q000494:pharmacology; D001681:Biological Assay / Q000379:methods*; D002460:Cell Line; D016360:Clostridioides difficile / Q000737:chemistry* / Q000276:immunology; D003601:Cytotoxicity Tests, Immunologic / Q000379:methods*; D003603:Cytotoxins / Q000276:immunology / Q000302:isolation & purification* / Q000494:pharmacology; D012680:Sensitivity and Specificity; D013552:Swine
D000907:Antibodies, Bacterial; D001427:Bacterial Toxins; D003603:Cytotoxins