Transfection microarray and the applications.
Microarray transfection has been extensively studied for high-throughput functional analysis of mammalian cells. However, control of efficiency and reproducibility are the critical issues for practical use. By using solid-phase transfection accelerators and nano-scaffold, we provide a highly efficient and reproducible microarray-transfection device, "transfection microarray". The device would be applied to the limited number of available primary cells and stem cells not only for large-scale functional analysis but also reporter-based time-lapse cellular event analysis.
Miyake
Masato
M
National Institute of Advanced Industrial Science and Technology, 2-42 Aomi, Koto-ku, Tokyo, 135-0064, Japan. masato-miyake@aist.go.jp
Yoshikawa
Tomohiro
T
Fujita
Satoshi
S
Miyake
Jun
J
D000818:Animals; D002478:Cells, Cultured; D006801:Humans; D008954:Models, Biological; D049329:Nanostructures / Q000737:chemistry; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D014162:Transfection / Q000379:methods*