Generation of an ABCG2(GFPn-puro) transgenic line--a tool to study ABCG2 expression in mice.
The ATP-binding cassette (ABC) transporter 2 (ABCG2) is expressed by stem cells in many organs and in stem cells of solid tumors. These cells are isolated based on the side population (SP) phenotype, a Hoechst 3342 dye efflux property believed to be conferred by ABCG2. Because of the limitations of this approach we generated transgenic mice that express Nuclear GFP (GFPn) coupled to the Puromycin-resistance gene, under the control of ABCG2 promoter/enhancer sequences. We show that ABCG2 is expressed in neural progenitors of the developing forebrain and spinal cord and in embryonic and adult endothelial cells of the brain. Using the neurosphere assay, we isolated tripotent ABCG2-expressing neural stem cells from embryonic mouse brain. This transgenic line is a powerful tool for studying the expression of ABCG2 in many tissues and for performing functional studies in different experimental settings.
Orford
Michael
M
The Cyprus Institute of Neurology and Genetics, Airport Avenue, No. 6, Agios Dometios 2370, Nicosia, Cyprus.
Mean
Richard
R
Lapathitis
George
G
Genethliou
Nicholas
N
Panayiotou
Elena
E
Panayi
Helen
H
Malas
Stavros
S
D000070996:ATP Binding Cassette Transporter, Subfamily G; D018528:ATP-Binding Cassette Transporters / Q000096:biosynthesis* / Q000235:genetics; D000483:Alleles; D000818:Animals; D000964:Antimetabolites, Antineoplastic / Q000494:pharmacology; D001921:Brain / Q000166:cytology / Q000196:embryology / Q000378:metabolism*; D002467:Cell Nucleus / Q000378:metabolism; D019008:Drug Resistance, Neoplasm / Q000235:genetics; D004742:Enhancer Elements, Genetic; D049452:Green Fluorescent Proteins / Q000096:biosynthesis / Q000235:genetics; D051379:Mice; D008822:Mice, Transgenic; D011401:Promoter Regions, Genetic; D011691:Puromycin / Q000494:pharmacology
D000070996:ATP Binding Cassette Transporter, Subfamily G; D018528:ATP-Binding Cassette Transporters; C420604:Abcg3 protein, mouse; D000964:Antimetabolites, Antineoplastic; D049452:Green Fluorescent Proteins; D011691:Puromycin