Assessment of cadaveric organ viability during pulsatile perfusion using infrared imaging.
Assessment of pulsatile perfusion (PP) is limited to measurements of flow (V) and resistance (R). We investigated infrared (IR) imaging during PP as a means for precise organ assessment. IR was used to monitor 10 porcine kidneys during 18 hr of PP in an uncontrolled Donation after Cardiac Death model. An IR camera (Lockheed Martin) was focused on the anterior surfaces of the kidneys. The degree of temperature homogeneity was compared with standard measurements of V and R. IR thermal images correlated with V and R (R=0.92, P<0.001). IR detected an increase in homogeneity during PP by comparing standard deviation differences before and after PP (P=0.002), which was not evident by standard measurements of V and R. Finally, IR assessment allowed for measurement of dynamic changes in perfusion.
Gorbach
Alexander M
AM
Bioengineering and Physical Science, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, MD, USA.
Leeser
David B
DB
Wang
Hengliang
H
Tadaki
Douglas K
DK
Fernandez
Carlos
C
Destephano
David
D
Hale
Douglas
D
Kirk
Allan D
AD
Gage
Fred A
FA
Elster
Eric A
EA
D000818:Animals; D001783:Blood Flow Velocity; D001831:Body Temperature; D002102:Cadaver; D002470:Cell Survival / Q000502:physiology* / Q000528:radiation effects; D006801:Humans; D007259:Infrared Rays; D007668:Kidney / Q000502:physiology*; D016030:Kidney Transplantation / Q000502:physiology; D018579:Patient Selection; D011673:Pulsatile Flow / Q000502:physiology*; D012079:Renal Circulation; D013552:Swine; D014019:Tissue Donors; D014184:Transplantation, Homologous / Q000502:physiology; D014655:Vascular Resistance