Determination of chloramphenicol, thiamphenicol, florfenicol, and florfenicol amine in poultry and porcine muscle and liver by gas chromatography-negative chemical ionization mass spectrometry.
A sensitive and reliable method using gas chromatography-negative chemical ionization mass spectrometry (GC-NCI/MS) was developed for the simultaneous determination of chloramphenicol (CAP), thiamphenicol (TAP), florfenicol (FF), and florfenicol amine (FFA) at trace levels in muscle and liver. Before extraction with ethyl acetate, CAP-d(5) was added to tissue samples as internal standard. The organic extracts were frozen to remove lipid and further purified by liquid-liquid extraction (LLE) with hexane and solid-phase extraction (SPE) using Oasis HLB cartridges. The target compounds were derivatized with BSTFA+1% TMCS prior to GC-NCI/MS determination in selected ion monitoring mode (SIM). The recovery values ranged from 78.5 to 105.5%, with relative standard deviations (RSD) <17%. The limits of detections (LODs) of 0.1 microg/kg for CAP and 0.5 microg/kg for TAP, FF, and FFA were obtain. Incurred sample and samples from local market were successfully analyzed using this method.
Shen
Jianzhong
J
Department of Veterinary Pharmacology and Toxicology, College of Veterinary Medicine, China Agricultural University, Beijing, People's Republic of China.
Xia
Xi
X
Jiang
Haiyang
H
Li
Cun
C
Li
Jiancheng
J
Li
Xiaowei
X
Ding
Shuangyang
S
D000818:Animals; D002701:Chloramphenicol / Q000031:analogs & derivatives* / Q000032:analysis*; D002849:Chromatography, Gas / Q000379:methods*; D008099:Liver / Q000737:chemistry*; D009132:Muscles / Q000737:chemistry*; D011200:Poultry; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D013552:Swine; D013839:Thiamphenicol / Q000031:analogs & derivatives / Q000032:analysis
D002701:Chloramphenicol; C083312:florfenicol amine; C035534:florfenicol; D013839:Thiamphenicol