Methiocarb-induced oxidative damage following subacute exposure and the protective effects of vitamin E and taurine in rats.
Methiocarb, is used worldwide in agriculture and health programs. Besides its advantages in the agriculture, it causes several toxic effects. In this study, we aimed to investigate subacute effects of methiocarb on lipid peroxidation, reduced glutathione (GSH), antioxidant enzymes such as superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px) and glutathione reductase (GSH-Rd) and histopathological changes in rat tissues. Moreover, we examined the possible protective effects of vitamin E and taurine on methiocarb-induced oxidative damage in rat tissues. Rats were randomly divided into six groups as follows; I-control group; II-methiocarb group; III-vitamin E group; IV-vitamin E+methiocarb group; V-taurine group and VI-taurine+methiocarb group. Methiocarb significantly increased lipid peroxidation in liver and kidney when compared to control groups. Levels of GSH and activities of SOD, CAT and GSH-Px were found to be decreased, while GSH-Rd remained unchanged in rat liver and kidney treated with methiocarb. Pretreatment of vitamin E and taurine resulted in a significant decrease on lipid peroxidation, alleviating effects on GSH and antioxidant enzymes. The degenerative histological changes were less in liver than kidney of rats treated with methiocarb. Pretreatment of vitamin E and taurine showed a protective effect on the histological changes in kidney comparing to the liver of rats treated with methiocarb.
Ozden
Sibel
S
Department of Pharmaceutical Toxicology, Faculty of Pharmacy, Istanbul University, 34116 Beyazit, Istanbul, Turkey.
Catalgol
Betul
B
Gezginci-Oktayoglu
Selda
S
Arda-Pirincci
Pelin
P
Bolkent
Sehnaz
S
Alpertunga
Buket
B
D000818:Animals; D000975:Antioxidants / Q000378:metabolism / Q000494:pharmacology*; D056486:Chemical and Drug Induced Liver Injury / Q000201:enzymology / Q000473:pathology / Q000517:prevention & control; D005978:Glutathione / Q000378:metabolism; D007306:Insecticides / Q000037:antagonists & inhibitors* / Q000633:toxicity*; D007674:Kidney Diseases / Q000139:chemically induced / Q000473:pathology / Q000517:prevention & control; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D008714:Methiocarb / Q000037:antagonists & inhibitors* / Q000633:toxicity*; D018384:Oxidative Stress / Q000187:drug effects*; D051381:Rats; D017208:Rats, Wistar; D013654:Taurine / Q000494:pharmacology*; D014810:Vitamin E / Q000494:pharmacology*
D000975:Antioxidants; D007306:Insecticides; D014810:Vitamin E; D013654:Taurine; D005978:Glutathione; D008714:Methiocarb