title: High dietary taurine reduces apoptosis and atherosclerosis in the left main coronary artery: association with reduced CCAAT/enhancer binding protein homologous protein and total plasma homocysteine but not lipidemia.
abstract: We sought to determine whether taurine could specifically protect against coronary artery disease during an atherogenic diet and whether taurine affects the lipid profile, metabolites of methionine, and endothelial atherogenic systems. Rabbits were fed one of the following diets for 4 weeks: (1) control diet; (2) 0.5% cholesterol+1.0% methionine; or (3) 0.5% cholesterol+1.0% methionine+2.5% taurine. Endothelial function was examined, and the left main coronary artery atherosclerosis was quantified by stereology and semiquantitative immunohistochemistry to determine the endothelial expression of proteins related to the NO, renin-angiotensin, endoplasmic reticulum, and oxidative stress systems, as well as apoptosis. Taurine normalized hyperhomocysteinemia (P<0.05) and significantly reduced hypermethioninemia (P<0.05) but not lipidemia. The intima:media ratio was reduced by 28% (P=0.034), and atherosclerosis was reduced by 64% (P=0.012) and endothelial cell apoptosis by 30% (P<0.01). Endothelial cell CCAAT/enhancer binding protein homologous protein was normalized (P<0.05). Taurine failed to improve hyperlipidemia, endothelial function, or endothelial proteins related to the NO, renin-angiotensin, and oxidative stress systems. Taurine reduces left main coronary artery wall pathology associated with decreased plasma total homocysteine, methionine, apoptosis, and normalization of CCAAT/enhancer binding protein homologous protein. These results elucidate the antiapoptotic and antiatherogenic properties of taurine, possibly via normalization of endoplasmic reticulum stress.
authors.affiliation: Departments of Cardiology, University of Melbourne, Austin Health, Heidelberg, Australia. azulli@unimelb.edu.au
authors.forename: Anthony
authors.initials: A
authors.lastname: Zulli
authors.forename: Eza
authors.initials: E
authors.lastname: Lau
authors.forename: Bagus P P
authors.initials: BP
authors.lastname: Wijaya
authors.forename: Xin
authors.initials: X
authors.lastname: Jin
authors.forename: Komang
authors.initials: K
authors.lastname: Sutarga
authors.forename: Grace D
authors.initials: GD
authors.lastname: Schwartz
authors.forename: Jonathon
authors.initials: J
authors.lastname: Learmont
authors.forename: Peter J
authors.initials: PJ
authors.lastname: Wookey
authors.forename: Angelo
authors.initials: A
authors.lastname: Zinellu
authors.forename: Ciriaco
authors.initials: C
authors.lastname: Carru
authors.forename: David L
authors.initials: DL
authors.lastname: Hare
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D023081:CCAAT-Binding Factor / Q000187:drug effects / Q000378:metabolism*; D003324:Coronary Artery Disease / Q000473:pathology* / Q000517:prevention & control; D004036:Diet, Atherogenic; D019587:Dietary Supplements; D004195:Disease Models, Animal; D004730:Endothelium, Vascular / Q000187:drug effects / Q000473:pathology; D006710:Homocysteine / Q000097:blood*; D006949:Hyperlipidemias / Q000503:physiopathology; D008297:Male; D008715:Methionine / Q000494:pharmacology; D009569:Nitric Oxide / Q000378:metabolism; D018384:Oxidative Stress; D011336:Probability; D011817:Rabbits; D011897:Random Allocation; D012016:Reference Values; D012680:Sensitivity and Specificity; D013654:Taurine / Q000494:pharmacology*
chemical_list: D023081:CCAAT-Binding Factor; D006710:Homocysteine; D013654:Taurine; D009569:Nitric Oxide; D008715:Methionine