title: Protective effect of hyperbaric oxygen therapy on experimental brain contusions.
abstract: BACKGROUND
  We evaluated the effect of hyperbaric oxygen therapy (HBO) on experimental brain contusions in rats using magnetic resonance imaging (MRI).
  
  
  METHODS
  Ten Sprague-Dawley rats were investigated at 24 h and 72 h after controlled cortical impact injury. One hour after trauma, 5 rats were treated for 60 min with 100% oxygen at 2.5 absolute atmosphere (ATA), 5 were kept at normobaric room air. MRI was performed longitudinally at 24 h and 72 h after injury. Lesion volume was determined in T2 weighted MRI scans. Relative apparent diffusion coefficient (ADC) changes were calculated in comparison to the contralateral side.
  
  
  RESULTS
  Following HBO, T2 lesion volume was smaller at 24 h versus controls (63.1 +/- 16.5 mm3 vs. 87.4 +/- 13.8 mm3, p < 0.05), and decreased further at 72 h (46.8 +/- 17.8 mm3 vs. 92.5 +/- 13.1 mm3, p < 0.01). At 24 h, the mean relative ADC change in the lesion area decreased from + 26.8 +/- 2.3% in controls to + 2.3 +/- 12.2% in HBO animals (p < 0.01). At 72 h, the HBO effect on relative ADC values was less when compared to 24 h.
  
  
  CONCLUSIONS
  A 60-minute exposure to hyperbaric oxygen starting 1 h after impact injury significantly attenuated lesion growth and relative increase of ADC values within the contused area for up to 72 h. Thus, a "single-shot" HBO treatment seems to have long-lasting neuroprotective effects on the contused brain and its penumbra.
authors.affiliation: Department of Neurosurgery, University of Leipzig, 04103 Leipzig, Germany. dat_conny@web.de
authors.forename: Cornelia
authors.initials: C
authors.lastname: Voigt
authors.forename: Annette
authors.initials: A
authors.lastname: Förschler
authors.forename: Matthias
authors.initials: M
authors.lastname: Jaeger
authors.forename: Jürgen
authors.initials: J
authors.lastname: Meixensberger
authors.forename: Lea
authors.initials: L
authors.lastname: Küppers-Tiedt
authors.forename: Martin U
authors.initials: MU
authors.lastname: Schuhmann
mesh_terms: D000818:Animals; D001930:Brain Injuries / Q000473:pathology / Q000628:therapy*; D004058:Diffusion; D004195:Disease Models, Animal; D006931:Hyperbaric Oxygenation / Q000379:methods*; D008279:Magnetic Resonance Imaging / Q000379:methods; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley