title: RGD modified PLGA/gelatin microspheres as microcarriers for chondrocyte delivery.
abstract: Poly(lactide-co-glycotide) (PLGA)/gelatin composite microspheres were prepared by an emulsion solvent evaporation technique. RGDS peptides were further immobilized under the catalyzation of water soluble carbodiimide (EDAC). Confocal laser scanning microscopy and transmission electron microscopy revealed that the gelatin was entrapped in the PLGA/gelatin microspheres with a manner of separated domains. The contents of the entrapped gelatin and immobilized RGDS peptides were quantified as 0.9 mg/20 mg and approximately 2.1 microg/20 mg microspheres by hydroxyproline analysis and bicinchoninic acid protein assay, respectively. Moreover, difference in morphology of PLGA, PLGA/gelatin and RGDS modified PLGA/gelatin (PLGA/gelatin-RGDS) microspheres was observed by scanning electron microscopy. The PLGA/gelatin and PLGA/gelatin-RGDS microspheres lost their weight rapidly in PBS, but slowly in DMEM/fetal bovine serum. Rabbit auricular chondrocytes were seeded onto the microspheres in vitro to assess their biological performance and applicability as cell carriers. Results show that amongst the PLGA, PLGA/gelatin and PLGA/gelatin-RGDS microspheres, the latter ones have the best performance in terms of chondrocyte attachment, proliferation, viability and sulfated glycosaminoglycans secretion.
authors.affiliation: Key Laboratory of Macromolecular Synthesis and Functionalization, Ministry of Education, Department of Polymer Science and Engineering, Zhejiang University, Hangzhou 310027, China.
authors.forename: Huaping
authors.initials: H
authors.lastname: Tan
authors.forename: Dejuan
authors.initials: D
authors.lastname: Huang
authors.forename: Lihong
authors.initials: L
authors.lastname: Lao
authors.forename: Changyou
authors.initials: C
authors.lastname: Gao
mesh_terms: D000818:Animals; D002417:Cattle; D002478:Cells, Cultured; D019902:Chondrocytes / Q000166:cytology* / Q000378:metabolism; D004337:Drug Carriers / Q000737:chemistry*; D005780:Gelatin / Q000737:chemistry*; D006025:Glycosaminoglycans / Q000737:chemistry / Q000378:metabolism; D019344:Lactic Acid / Q000737:chemistry*; D008422:Materials Testing; D008855:Microscopy, Electron, Scanning; D008863:Microspheres; D009842:Oligopeptides / Q000737:chemistry*; D010316:Particle Size; D011100:Polyglycolic Acid / Q000737:chemistry*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011817:Rabbits
chemical_list: D004337:Drug Carriers; D006025:Glycosaminoglycans; D009842:Oligopeptides; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; C047981:arginyl-glycyl-aspartic acid; D005780:Gelatin