title: OH-2, a hyperdiploid myeloma cell line without an IGH translocation, has a complex translocation juxtaposing MYC near MAFB and the IGK locus.
abstract: Multiple myeloma can be classified into hyperdiploid (HRD) (with 48-74 chromosomes) and non-hyperdiploid tumors (usually with immunoglobulin heavy chain translocations). The OH-2 human myeloma cell line (HMCL) retains the same HRD genotype as the primary tumor, with extra copies of chromosomes 3, 7, 15, 19, and 21. Both OH-2 and primary cells have a complex secondary translocation in which the IGK 3' enhancer is inserted between MYC and MAFB, resulting in dysregulation of both oncogenes. OH-2 provides a unique example of an HMCL and the corresponding primary tumor that are shown to share the same HRD genotype.
authors.affiliation: Department of Cancer Research and Molecular Medicine, Faculty of Medicine, Norwegian University of Science and Technology, Trondheim, Norway. thea.k.vatsveen@ntnu.no
authors.forename: Thea Kristin
authors.initials: TK
authors.lastname: Våtsveen
authors.forename: Erming
authors.initials: E
authors.lastname: Tian
authors.forename: Stine H
authors.initials: SH
authors.lastname: Kresse
authors.forename: Leonardo A
authors.initials: LA
authors.lastname: Meza-Zepeda
authors.forename: Ana
authors.initials: A
authors.lastname: Gabrea
authors.forename: Oleg
authors.initials: O
authors.lastname: Glebov
authors.forename: Hong Yan
authors.initials: HY
authors.lastname: Dai
authors.forename: Anders
authors.initials: A
authors.lastname: Sundan
authors.forename: W Michael
authors.initials: WM
authors.lastname: Kuehl
authors.forename: Magne
authors.initials: M
authors.lastname: Børset
mesh_terms: D045744:Cell Line, Tumor; D004171:Diploidy; D004742:Enhancer Elements, Genetic; D016259:Genes, myc; D005838:Genotype; D006801:Humans; D007143:Immunoglobulin Heavy Chains / Q000235:genetics*; D007145:Immunoglobulin kappa-Chains / Q000235:genetics*; D017404:In Situ Hybridization, Fluorescence; D007621:Karyotyping; D051258:MafB Transcription Factor / Q000235:genetics*; D009101:Multiple Myeloma / Q000235:genetics* / Q000473:pathology; D012333:RNA, Messenger / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014178:Translocation, Genetic
chemical_list: D007143:Immunoglobulin Heavy Chains; D007145:Immunoglobulin kappa-Chains; C490407:MAFB protein, human; D051258:MafB Transcription Factor; D012333:RNA, Messenger