title: An iron-regulated LysR-type element mediates antimicrobial peptide resistance and virulence in Yersinia pseudotuberculosis.
abstract: During the course of its infection of the mammalian digestive tract, the entero-invasive, Gram-negative bacterium Yersinia pseudotuberculosis must overcome various hostile living conditions (notably, iron starvation and the presence of antimicrobial compounds produced in situ). We have previously reported that in vitro bacterial growth during iron deprivation raises resistance to the antimicrobial peptide polymyxin B; here, we show that this phenotype is mediated by a chromosomal gene (YPTB0333) encoding a transcriptional regulator from the LysR family. We determined that the product of YPTB0333 is a pleiotropic regulator which controls (in addition to its own expression) genes encoding the Yfe iron-uptake system and polymyxin B resistance. Lastly, by using a mouse model of oral infection, we demonstrated that YPTB0333 is required for colonization of Peyer's patches and mesenteric lymph nodes by Y. pseudotuberculosis.
authors.affiliation: Inserm U801, F-59019 Lille, France.|Institut Pasteur de Lille, F-59019 Lille, France.|CHULille, F-59000 Lille, France.|Université Lille Nord de France, F-59000 Lille, France.
authors.forename: Sonia
authors.initials: S
authors.lastname: Arafah
authors.affiliation: CHULille, F-59000 Lille, France.|Inserm U801, F-59019 Lille, France.|Institut Pasteur de Lille, F-59019 Lille, France.|Université Lille Nord de France, F-59000 Lille, France.
authors.forename: Marie-Laure
authors.initials: ML
authors.lastname: Rosso
authors.affiliation: Department of Microbiology and Immunology, Center for Microbial Diseases and Immunity Research, Lower Mall Research Station, University of British Columbia, Vancouver, BC V6T 1Z4, Canada.
authors.forename: Linda
authors.initials: L
authors.lastname: Rehaume
authors.affiliation: Department of Microbiology and Immunology, Center for Microbial Diseases and Immunity Research, Lower Mall Research Station, University of British Columbia, Vancouver, BC V6T 1Z4, Canada.
authors.forename: Robert E W
authors.initials: REW
authors.lastname: Hancock
authors.affiliation: CHULille, F-59000 Lille, France.|Inserm U801, F-59019 Lille, France.|Institut Pasteur de Lille, F-59019 Lille, France.|Université Lille Nord de France, F-59000 Lille, France.
authors.forename: Michel
authors.initials: M
authors.lastname: Simonet
authors.affiliation: Inserm U801, F-59019 Lille, France.|Institut Pasteur de Lille, F-59019 Lille, France.|Université Lille Nord de France, F-59000 Lille, France.|CHULille, F-59000 Lille, France.
authors.forename: Michaël
authors.initials: M
authors.lastname: Marceau
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D024881:Drug Resistance, Bacterial; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D007501:Iron / Q000378:metabolism*; D051379:Mice; D009875:Operator Regions, Genetic; D010581:Peyer's Patches / Q000382:microbiology; D011112:Polymyxin B / Q000494:pharmacology*; D015202:Protein Engineering; D014157:Transcription Factors / Q000096:biosynthesis* / Q000172:deficiency / Q000235:genetics; D014774:Virulence; D015008:Yersinia enterocolitica / Q000187:drug effects / Q000378:metabolism; D015011:Yersinia pseudotuberculosis / Q000187:drug effects / Q000378:metabolism / Q000472:pathogenicity; D015012:Yersinia pseudotuberculosis Infections / Q000382:microbiology*
chemical_list: D000900:Anti-Bacterial Agents; D014157:Transcription Factors; D007501:Iron; D011112:Polymyxin B