Direct effects of activin A on the activation of mouse macrophage RAW264.7 cells.
Macrophages play critical roles in innate immune and acquired immune via secreting pro-inflammatory mediators, phagocytosing microorganisms and presenting antigens. Activin A, a member of transforming growth factor beta (TGF-beta) superfamily, is produced by macrophages and microglia cells. In this study, we reported a direct effect of activin A as a pro-inflammatory factor on mouse macrophage cell line RAW264.7 cells. Our data revealed that activin A could not only increase IL-1 beta and IL-6 production from RAW264.7 cells, but also promote pinocytic and phagocytic activities of RAW264.7 cells. In addition, activin A obviously up-regulated MHC II expression on the surface of RAW264.7 cells, whereas did not influence MHC I expression. Activin A also enhanced CD80 expression, which is a marker of activated macrophages, but did not influence RAW264.7 cell proliferation. These data suggest that activin A may regulate primary macrophage-mediated innate and acquired immune response via promoting the activation of rest macrophages.
Ge
Jingyan
J
Department of Immunology, Norman Bethune College of Medicine, Jilin University, Changchun 130021, China.
Wang
Yinan
Y
Feng
Ye
Y
Liu
Haiyan
H
Cui
Xueling
X
Chen
Fangfang
F
Tai
Guixiang
G
Liu
Zhonghui
Z
D028341:Activins / Q000276:immunology* / Q000378:metabolism; D000818:Animals; D000943:Antigens, Differentiation; D018122:B7-1 Antigen / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D002460:Cell Line; D005786:Gene Expression Regulation; D005802:Genes, MHC Class II / Q000276:immunology; D018836:Inflammation Mediators / Q000276:immunology* / Q000378:metabolism; D053583:Interleukin-1beta / Q000378:metabolism; D015850:Interleukin-6 / Q000378:metabolism; D008262:Macrophage Activation / Q000276:immunology*; D008264:Macrophages / Q000166:cytology / Q000276:immunology / Q000378:metabolism*; D051379:Mice; D010587:Phagocytosis / Q000276:immunology; D010873:Pinocytosis / Q000276:immunology
D000943:Antigens, Differentiation; D018122:B7-1 Antigen; D018836:Inflammation Mediators; D053583:Interleukin-1beta; D015850:Interleukin-6; C422405:activin A; D028341:Activins