title: Pancreatic response in healthy dogs fed diets of various fat compositions.
abstract: OBJECTIVE
  To indirectly assess the pancreatic response in healthy dogs that were fed diets of different fat compositions with or without supplemental pancreatic enzymes and medium-chain triglycerides (MCTs).
  
  
  METHODS
  10 healthy adult dogs.
  
  
  METHODS
  Dogs were fed 4 diets once in random order at 1-week intervals; food was withheld from the dogs for > or = 12 hours prior to the feeding of each diet. Diets A and B contained 16% and 5% crude fat, respectively; diet C was composed of diet A with pancreatic enzymes; and diet D was composed of diet B with pancreatic enzymes and MCTs. Serum canine trypsin-like immunoreactivity (cTLI) and canine pancreatic lipase immunoreactivity (cPLI) concentrations were measured before (0 hours) and at 1 to 2 and 6 hours after feeding. Serum gastrin concentration was measured at 0 hours and at 5 to 10 minutes and 1 to 2 hours after feeding. A gastrin assay validation study was performed to confirm accuracy of test results in dogs. Data were analyzed by use of a repeated-measures general ANOVA.
  
  
  RESULTS
  Serum cTLI, cPLI, or gastrin concentrations in the dogs did not differ among the different diets fed, among dogs, or over time. When multiple comparisons were analyzed, diet D caused the least amount of measurable pancreatic response, although this difference was not significant.
  
  
  CONCLUSIONS
  Results did not indicate a significant effect of dietary fat content or addition of supplemental MCT oil or pancreatic enzymes in diets on serum cTLI, cPLI, or gastrin concentrations in healthy dogs.
authors.affiliation: Department of Veterinary Clinical Sciences, Murdoch University, Perth, WA, Australia 6150.
authors.forename: Fleur E
authors.initials: FE
authors.lastname: James
authors.forename: Caroline S
authors.initials: CS
authors.lastname: Mansfield
authors.forename: Jörg M
authors.initials: JM
authors.lastname: Steiner
authors.forename: David A
authors.initials: DA
authors.lastname: Williams
authors.forename: Ian D
authors.initials: ID
authors.lastname: Robertson
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D004032:Diet / Q000662:veterinary*; D004041:Dietary Fats / Q000008:administration & dosage*; D019587:Dietary Supplements; D004285:Dogs / Q000502:physiology*; D004798:Enzymes / Q000008:administration & dosage; D005260:Female; D005755:Gastrins / Q000097:blood; D008049:Lipase / Q000097:blood; D008297:Male; D010179:Pancreas / Q000502:physiology*; D011897:Random Allocation; D015203:Reproducibility of Results; D014280:Triglycerides / Q000008:administration & dosage*; D014357:Trypsin / Q000097:blood
chemical_list: D004041:Dietary Fats; D004798:Enzymes; D005755:Gastrins; D014280:Triglycerides; D008049:Lipase; D014357:Trypsin