High throughput sequencing technology reveals that the taxoid elicitor methyl jasmonate regulates microRNA expression in Chinese yew (Taxus chinensis).
MicroRNAs (miRNAs) are important regulators of gene expression that are increasing being implicated in controlling plant development and its interaction with the environment. The advent of new high-throughput sequencing technologies has enabled both the discovery and quantification of miRNAs from a diverse range of species. In this study, we employed high throughput Illumina sequencing to identify miRNAs from Taxus chinensis (T. chinensis) cells to investigate the effect of the taxoid elicitor methyl jasmonate (MJ) on miRNA expression. In a dataset of approximately 6.6 million sequences, a total of 58 miRNAs, belonging to 25 families were identified. A majority of them are conserved between angiosperms and gymnosperms. However, two miRNAs (miR1310 and miR1314) appear gymnosperm-specific, with miR1314 likely to exist as a cluster. MJ treatment significantly affected the expression of specific miRNAs; 14 miRNAs from 7 different families (miR156, miR168, miR169, miR172, miR396, miR480 and mir1310) were down regulated whereas 3 miRNAs from 2 families (miR164 and miR390) were up regulated.
Qiu
Deyou
D
Department of Molecular Biology, The Research Institute of Forestry, Chinese Academy of Forestry, Beijing, China. qiudy@caf.ac.cn
Pan
Xiaoping
X
Wilson
Iain W
IW
Li
Fenglan
F
Liu
Min
M
Teng
Wenjing
W
Zhang
Baohong
B
D000085:Acetates / Q000494:pharmacology*; D002460:Cell Line; D002851:Chromatography, High Pressure Liquid; D003517:Cyclopentanes / Q000494:pharmacology*; D018506:Gene Expression Regulation, Plant / Q000187:drug effects*; D035683:MicroRNAs / Q000737:chemistry / Q000235:genetics*; D054883:Oxylipins / Q000494:pharmacology*; D010937:Plant Growth Regulators / Q000494:pharmacology; D018749:RNA, Plant / Q000737:chemistry / Q000235:genetics; D017423:Sequence Analysis, RNA / Q000379:methods*; D043823:Taxoids / Q000032:analysis / Q000378:metabolism*; D020946:Taxus / Q000166:cytology / Q000235:genetics / Q000378:metabolism
D000085:Acetates; D003517:Cyclopentanes; D035683:MicroRNAs; D054883:Oxylipins; D010937:Plant Growth Regulators; D018749:RNA, Plant; D043823:Taxoids; C072239:methyl jasmonate