title: Residue depletion of albendazole and its metabolites in the muscle tissue of large mouth and hybrid striped bass after oral administration.
abstract: The residue depletion profiles of albendazole (ABZ) and its major metabolites: albendazole sulfoxide (ABZ-SO), albendazole sulfone (ABZ-SO(2)) and albendazole aminosulfone (ABZ-2-NH(2)SO(2)) were studied in the muscle tissues of large mouth (LMB) and hybrid striped bass (HSB). A single oral dose of 10mg/kg albendazole was given to the two fish species via intra-gastric tube. The muscle tissues with adhering skin were collected at 8, 16, 24, 48, 72, 96 and 120h post dose from both species. The samples were homogenized in dry ice and subjected to extraction and cleanup procedures. The final sample extracts were analyzed by high performance liquid chromatography. The results indicate that both ABZ and its pharmacologically active metabolite ABZ-SO were retained longer in LMB than in HSB after oral treatment. Albendazole was detectable until 8h or 6.7 degree days ( degrees D) and 48h (40 degrees D) in HSB and LMB, respectively. However, ABZ-SO was detectable up to 48h (40 degrees D) and 96h (80 degrees D) in HSB and LMB, respectively. Among the inactive metabolites, ABZ-SO(2) was present in both fish species; however, ABZ-2-NH(2)SO(2) was detected only in LMB.
authors.affiliation: Food and Drug Administration, Center for Veterinary Medicine, Office of Research, Laurel, MD 20708, USA. Badaruddin.Shaikh@fda.hhs.gov
authors.forename: Badar
authors.initials: B
authors.lastname: Shaikh
authors.forename: Nathan
authors.initials: N
authors.lastname: Rummel
authors.forename: Charles
authors.initials: C
authors.lastname: Gieseker
authors.forename: Christie-Sue
authors.initials: CS
authors.lastname: Cheely
authors.forename: Renate
authors.initials: R
authors.lastname: Reimschuessel
mesh_terms: D000284:Administration, Oral; D015766:Albendazole / Q000008:administration & dosage / Q000032:analysis* / Q000378:metabolism*; D000818:Animals; D001492:Bass; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004350:Drug Residues / Q000032:analysis* / Q000378:metabolism; D009132:Muscles / Q000737:chemistry* / Q000378:metabolism
chemical_list: D015766:Albendazole