Two-colour two-photon confocal microscopy with isotropic three-dimensional resolution and parallel excitation.
We demonstrate a novel design of two-colour two-photon fluorescence microscope in which isotropic three-dimensional imaging resolution and high scanning speed can be achieved simultaneously. In our scheme, a three-dimensional optical lattice constructed by multi-beam interference is used for two-colour two-photon fluorescence excitation. Our simulation results show that a resolution of 113.5 nm can be achieved in both transverse and axial directions with two pump pulses at the wavelengths of 400 and 800 nm, respectively; meanwhile, imaging speed can be greatly improved compared with that of traditional two-photon scanning fluorescence microscopes.
Ni
J
J
State Key Laboratory of High Field Laser Physics, Shanghai Institute of Optics and Fine Mechanics, Chinese Academy of Science, P.O. Box 800-211, Shanghai 201800, China.
Qiao
L
L
Wang
C
C
Zhao
F
F
Cheng
Y
Y
Xu
Z
Z