Identification of Streptococcus agalactiae isolates from various phylogenetic lineages by matrix-assisted laser desorption ionization-time of flight mass spectrometry.
Variations in proteins related to bacterial diversity may affect species identification performed using matrix-assisted laser desorption ionization (MALDI)-time of flight mass spectrometry. Using this method, we identified 110 Streptococcus agalactiae isolates characterized by serotyping and multilocus sequence typing. Serotype III and sequence type 23 strains expressed the widest variation in molecular weight of putative "species-identifying" biomarker ions. Recognition of the diversity of MALDI patterns observed in strains that represent all major intraspecies lineages assists in the constitution of an optimal reference database.
Lartigue
Marie-Frédérique
MF
Université François-Rabelais de Tours, UFR de Médecine, EA 3854 Bactéries et risque materno-foetal, Tours, France. lartigue@med.univ-tours.fr
Héry-Arnaud
Geneviève
G
Haguenoer
Eve
E
Domelier
Anne-Sophie
AS
Schmit
Pierre-Olivier
PO
van der Mee-Marquet
Nathalie
N
Lanotte
Philippe
P
Mereghetti
Laurent
L
Kostrzewa
Markus
M
Quentin
Roland
R
D001426:Bacterial Proteins / Q000032:analysis*; D016000:Cluster Analysis; D016172:DNA Fingerprinting; D005260:Female; D014644:Genetic Variation; D005838:Genotype; D006801:Humans; D012703:Serotyping; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D013292:Streptococcus agalactiae / Q000737:chemistry* / Q000145:classification / Q000302:isolation & purification*
D001426:Bacterial Proteins