Enhanced dermal wound neovascularization by targeted delivery of endothelial progenitor cells using an RGD-g-PLLA scaffold.
Endothelial progenitor cells (EPCs), endothelial precursors that promote neovascularization in ischemic tissues, have shown the limited vascular regeneration efficacy due to their poor homing into injured sites and low survival, so that a variety of biosynthetic scaffolds have been employed as cell delivery vehicles to overcome the current cell transplantation methods. However, few paralleled studies that directly compare the efficacy of EPCs seeded within synthetic scaffolds to that of EPCs delivered by the conventional transplantation techniques used for EPC therapies have been performed. To address these issues, RGD-g-PLLA biosynthetic scaffold was developed for the targeted EPC delivery and was found to successfully support the in vitro growth and endothelial functions of EPCs. This scaffold also appeared to be good as in vivo targeted delivery carriers of EPCs as it promoted vascular regeneration in a murine dermal wound models. Furthermore, direct comparison with the intradermal EPC injection revealed that the targeted delivery of EPCs by using the RGD-g-PLLA scaffold was superior to their conventional local injection method in terms of the localization and survival/retention of the transplanted EPCs, and their vascular repairing potential. These results suggest that the development of an effective stem cell delivery system may help to maximize the tissue-repairing efficacy with a limited number of stem cells, thereby resolving the limited clinical success of current stem cell therapies that have utilized simple cell injections or infusions.
Kim
Koung Li
KL
Department of Medicine, Samsung Medical Center, Samsung Biomedical Research Institute, Sungkyunkwan University School of Medicine, 50 Ilwon-dong, Kangnam-ku, Seoul 135-710, Republic of Korea.
Han
Dong Keun
DK
Park
Kwideok
K
Song
Sun-Hwa
SH
Kim
Ji Yeon
JY
Kim
Jeong-Min
JM
Ki
Ho Yun
HY
Yie
Se Won
SW
Roh
Cheong-Rae
CR
Jeon
Eun-Seok
ES
Kim
Duk-Kyung
DK
Suh
Wonhee
W
D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry / Q000378:metabolism; D002448:Cell Adhesion / Q000502:physiology; D020405:Dermis / Q000166:cytology / Q000473:pathology; D042783:Endothelial Cells / Q000166:cytology / Q000637:transplantation*; D019344:Lactic Acid / Q000737:chemistry / Q000378:metabolism*; D008297:Male; D008422:Materials Testing; D051379:Mice; D008819:Mice, Nude; D018919:Neovascularization, Physiologic; D009573:Nitrites / Q000378:metabolism; D009842:Oligopeptides / Q000737:chemistry / Q000378:metabolism*; D011091:Polyesters; D011108:Polymers / Q000737:chemistry / Q000378:metabolism*; D012038:Regeneration / Q000502:physiology; D033581:Stem Cell Transplantation; D054457:Tissue Scaffolds; D014945:Wound Healing
D001672:Biocompatible Materials; D009573:Nitrites; D009842:Oligopeptides; D011091:Polyesters; D011108:Polymers; D019344:Lactic Acid; C033616:poly(lactide); C047981:arginyl-glycyl-aspartic acid