Characterization of human skeletal muscle biopsy samples using shotgun proteomics.
We characterized the human muscle proteome by studying muscle biopsy specimens through four different workflows, using 1 or 2D peptide separation, SDS gels, or differential solubilization. By performing MS/MS analyses of 178 4-h LC separations derived from 31 patients, we identified more than 2000 proteins, and determined how 370 very abundant proteins behave upon differential solubilization. The resulting semiquantitative database should serve as a resource for muscle biochemistry.
Parker
Kenneth C
KC
Harvard Partners Center for Genetics and Genomics, Cambridge, MA, USA. kenneth.aprker@virgininstruments.com
Walsh
Ronan J
RJ
Salajegheh
Mohammad
M
Amato
Anthony A
AA
Krastins
Bryan
B
Sarracino
David A
DA
Greenberg
Steven A
SA
D001706:Biopsy; D002853:Chromatography, Liquid / Q000379:methods; D016000:Cluster Analysis; D030562:Databases, Protein; D015180:Electrophoresis, Gel, Two-Dimensional; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D013058:Mass Spectrometry; D018482:Muscle, Skeletal / Q000473:pathology*; D010455:Peptides / Q000737:chemistry; D040081:Protein Array Analysis; D020543:Proteome / Q000032:analysis / Q000378:metabolism; D040901:Proteomics / Q000379:methods*; D012518:Sarcomeres / Q000378:metabolism; D012995:Solubility
D010455:Peptides; D020543:Proteome