Fermentative hydrogen production from tofu-processing waste and anaerobic digester sludge using microbial consortium.
The combination of tofu-processing waste and anaerobic digester sludge was studied for its fermentative H(2) production capacity in batch and continuous modes using a thermophilic mixed culture. Heat-treatment (110 degrees C, 30 min) in the presence of 0.5% HCl increased the soluble carbohydrate content of the tofu waste from 2.2 to 10.4 g/l. Anaerobic digester sludge was added to the tofu waste for supplementary nutrients with the optimal mixing ratio of 20% (v/v) under batch conditions. In continuous experiments, the effects of HRT(hydraulic retention time) and pH were investigated for the ranges of 2-6h and 5.0-6.0, respectively. The maximal H(2) production rate (12.0 l H(2)/l/day) and yield (2.3 mol H(2)/mol glucose equivalent) were obtained at HRT 4h and pH 5.5 while maintaining the head space gas at 50-60% (v/v) of H(2) without CH(4). This study indicates that the combination of tofu-processing waste and digester sludge can be considered to be one of the most promising forms of organic waste for continuous H(2) production.
Kim
Mi-Sun
MS
Bioenergy Research Center, Korea Institute of Energy Research, Daejeon, Republic of Korea. bmmskim@kier.re.kr
Lee
Dong-Yeol
DY
D000693:Anaerobiosis; D001421:Bacteria, Anaerobic / Q000378:metabolism*; D001673:Biodegradation, Environmental; D019149:Bioreactors / Q000382:microbiology; D005285:Fermentation; D019649:Food Industry; D006358:Hot Temperature; D006859:Hydrogen / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D012037:Refuse Disposal / Q000379:methods*; D012722:Sewage / Q000737:chemistry / Q000382:microbiology; D045730:Soy Foods; D013997:Time Factors
D012722:Sewage; D006859:Hydrogen