Comparison of in vitro chondrogenic potential of human mesenchymal stem cells derived from bone marrow and adipose tissue.
In the present work, the human bone marrow and adipose tissue-derived mesenchymal stem cells (MSCs) were isolated and expanded under in vitro condition. After their phenotypic analysis, the chondrogenic differentiation was induced by using of the three-dimensional culture system without supplementation of growth factors, and their chondrogenic potential was compared. Obtained results proved that both types of MSCs undergo the process of chondrogenic differentiation. Comparative analysis showed that chondrogenic potential of adipose tissue-derived MSCs was slightly decreased in comparison with bone marrow-derived MSCs. However, both evaluated MSCs may play important role in the cartilage tissue engineering.
Danisovic
Lubos
L
Institute of Medical Biology and Genetics, Faculty of Medicine, Comenius University in Bratislava, Sasinkova 4, 811 08 Bratislava, Slovakia. lubos.danisovic@fmed.uniba.sk
Varga
Ivan
I
Polák
Stefan
S
Ulicná
Marcela
M
Hlavacková
Lívia
L
Böhmer
Daniel
D
Vojtassák
Ján
J
D000273:Adipose Tissue / Q000166:cytology / Q000502:physiology*; D000328:Adult; D053687:Adult Stem Cells / Q000166:cytology / Q000502:physiology*; D015703:Antigens, CD / Q000378:metabolism; D001854:Bone Marrow Cells / Q000166:cytology / Q000502:physiology*; D018929:Cell Culture Techniques; D002478:Cells, Cultured; D020219:Chondrogenesis / Q000502:physiology*; D024043:Collagen Type II / Q000378:metabolism; D006680:HLA Antigens / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000502:physiology*; D010641:Phenotype
D015703:Antigens, CD; D024043:Collagen Type II; D006680:HLA Antigens