Identification of cellular proteome modifications in response to West Nile virus infection.
Flaviviruses are positive-stranded RNA viruses that are a public health problem because of their widespread distribution and their ability to cause a variety of diseases in humans. West Nile virus is a mosquito-borne member of this genus and is the etiologic agent of West Nile encephalitis. Clinical manifestations of West Nile virus infection are diverse, and their pathogenic mechanisms depend on complex virus-cell interactions. In the present work, we used proteomics technology to analyze early Vero cell response to West Nile infection. The differential proteomes were resolved 24 h postinfection using two-dimensional DIGE followed by mass spectrometry identification. Quantitative analysis (at least 2-fold quantitative alteration, p < 0.05) revealed 127 differentially expressed proteins with 68 up-regulated proteins and 59 down-regulated proteins of which 93 were successfully identified. The implication for mammalian cellular responses to this neurotropic flavivirus infection was analyzed and made possible more comprehensive characterization of the virus-host interactions involved in pathogenesis. The present study thus provides large scale protein-related information that should be useful for understanding how the host metabolism is modified by West Nile infection and for identifying new potential targets for antiviral therapy.
Pastorino
Boris
B
Unité virologie tropicale, Institut de médecine tropicale du Service de santé des armées, Antenne Marseille de l'Institut de Recherche Biomédicale des Armées, Marseille, France.
Boucomont-Chapeaublanc
Elodie
E
Peyrefitte
Christophe N
CN
Belghazi
Maya
M
Fusaï
Thierry
T
Rogier
Christophe
C
Tolou
Hugues J
HJ
Almeras
Lionel
L
D000818:Animals; D002470:Cell Survival; D002522:Chlorocebus aethiops; D015180:Electrophoresis, Gel, Two-Dimensional; D006801:Humans; D008969:Molecular Sequence Data; D020543:Proteome / Q000032:analysis*; D040901:Proteomics / Q000379:methods; D053719:Tandem Mass Spectrometry; D014709:Vero Cells; D014779:Virus Replication; D014901:West Nile Fever / Q000378:metabolism*; D014902:West Nile virus / Q000378:metabolism*
D020543:Proteome