Effect of different regions of Nogo-A on the differentiation of neural progenitors.
Nogo-A is an inhibitor of neurite outgrowth and axonal regeneration after CNS injury. Several functional regions including Nogo-66 were identified to mediate the inhibitory effect of Nogo-A. We have reported that Nogo-66 could promote neural progenitors to differentiate into glial cells. Here we exam three other regions of Nogo-A and show two of them also mediate the differentiation of neural progenitors. A 172-residues N-terminal region and a 37-residues C-terminal region of Nogo-A both could inhibit neuronal differentiation and promoted glial cell formation. This study illustrated that Nogo-A had multiple functional domains on the behavior of neuronal cells. The inhibitory effect of neural differentiation of Nogo-A may also contribute to its restraint of CNS repair.
Gao
Yuan
Y
Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, 3 Nanyitiao, Zhongguancun, Beijing 100190, China.
Xiao
Zhifeng
Z
Chen
Bing
B
Wang
Bin
B
Han
Jin
J
Zhao
Yannan
Y
Zhang
Jing
J
Dai
Jianwu
J
D000818:Animals; D017931:DNA Primers; D005784:Gene Amplification; D017930:Genes, Reporter; D005904:Glial Fibrillary Acidic Protein / Q000235:genetics; D005982:Glutathione Transferase / Q000235:genetics / Q000378:metabolism; D009185:Myelin Proteins / Q000235:genetics* / Q000378:metabolism; D000070798:Nogo Proteins; D010446:Peptide Fragments / Q000235:genetics / Q000378:metabolism; D010957:Plasmids; D051381:Rats; D011994:Recombinant Proteins / Q000378:metabolism; D015183:Restriction Mapping; D020133:Reverse Transcriptase Polymerase Chain Reaction
D017931:DNA Primers; D005904:Glial Fibrillary Acidic Protein; D009185:Myelin Proteins; D000070798:Nogo Proteins; D010446:Peptide Fragments; D011994:Recombinant Proteins; C000603197:Rtn4 protein, rat; D005982:Glutathione Transferase