title: Tempol reduces bacterial translocation after ischemia/reperfusion injury in a rat model of superior mesenteric artery occlusion.
abstract: OBJECTIVE
  We investigated whether Tempol, a water-soluble antioxidant, prevents the harmful effects of superior mesenteric ischemia/reperfusion on intestinal tissues in rats.
  
  
  METHODS
  The rats were divided into three groups of 10. In group 1, the superior mesenteric artery (SMA) was isolated but not occluded, and in groups 2 and 3 the superior mesenteric artery was occluded for 60 min. After that, the clamp was removed and reperfusion began. In group 3, 5 min before the start of reperfusion, a bolus dose of 30 mg/kg Tempol was administered intravenously and continued at a dose of 30 mg/kg for 60 min. All animals were euthanized after 24 h and tissue samples were collected for analysis.
  
  
  RESULTS
  There was a significant increase in myeloperoxidase activity, malondialdehyde levels, and the incidence of bacterial translocation in group 2, with a decrease in glutathione levels. These parameters were found to be normalized in group 3. The intestinal mucosal injury score in group 2 was significantly higher than those in groups 1 and 3.
  
  
  CONCLUSIONS
  Tempol prevents bacterial translocation while precluding the harmful effects of ischemia/reperfusion injury on intestinal tissues in a rat model of superior mesenteric artery occlusion.
authors.affiliation: Department of General Surgery, Haydarpasa Numune Training and Research Hospital, Istanbul, Turkey.
authors.forename: Ibrahim
authors.initials: I
authors.lastname: Berber
authors.forename: Cagatay
authors.initials: C
authors.lastname: Aydin
authors.forename: Nural
authors.initials: N
authors.lastname: Cevahir
authors.forename: Cigdem
authors.initials: C
authors.lastname: Yenisey
authors.forename: Gulistan
authors.initials: G
authors.lastname: Gumrukcu
authors.forename: Goksel
authors.initials: G
authors.lastname: Kocbil
authors.forename: Gurkan
authors.initials: G
authors.lastname: Tellioglu
authors.forename: Koray
authors.initials: K
authors.lastname: Tekin
mesh_terms: D000208:Acute Disease; D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage / Q000494:pharmacology*; D001157:Arterial Occlusive Diseases / Q000473:pathology*; D018988:Bacterial Translocation / Q000187:drug effects*; D003497:Cyclic N-Oxides / Q000008:administration & dosage / Q000494:pharmacology*; D007413:Intestinal Mucosa / Q000187:drug effects / Q000473:pathology*; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D008638:Mesenteric Arteries / Q000187:drug effects / Q000473:pathology*; D023421:Models, Animal; D017202:Myocardial Ischemia / Q000150:complications* / Q000517:prevention & control; D015425:Myocardial Reperfusion / Q000009:adverse effects*; D009504:Neutrophils / Q000187:drug effects / Q000201:enzymology; D051381:Rats; D017208:Rats, Wistar; D017382:Reactive Oxygen Species; D013113:Spin Labels
chemical_list: D000975:Antioxidants; D003497:Cyclic N-Oxides; D017382:Reactive Oxygen Species; D013113:Spin Labels; D008315:Malondialdehyde; C001803:tempol