Novel fluorescence enhancement IgE assay using a DNA aptamer.
In this paper, we demonstrate a fluorescence immunoglobulin E (IgE) assay probe based on a DNA aptamer. A Texas red-labeled short DNA strand (T-DNA) complementary with part of the IgE aptamer sequence was used to produce the fluorescence enhancement effected upon the binding of IgE to the aptamer. Another short DNA strand labeled with dabcyl quencher (Q-DNA) complementary with part of the aptamer sequence nearby the T-DNA location was used to lower the background fluorescence. The IgE can be detected in the concentration range from 9.2 x 10(-11) to 3.7 x 10(-8) mol L(-1) with a detection limit of 5.7 x 10(-11) mol L(-1).
He
Jing-Lin
JL
State Key Laboratory for Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, PR China. rqyu@hnu.cn.
Wu
Zai-Sheng
ZS
Zhang
Song-Bai
SB
Shen
Guo-Li
GL
Yu
Ru-Qin
RQ
D052157:Aptamers, Nucleotide / Q000737:chemistry*; D015374:Biosensing Techniques; D004247:DNA / Q000737:chemistry*; D005453:Fluorescence; D007118:Immunoassay / Q000379:methods*; D007073:Immunoglobulin E / Q000737:chemistry*; D011485:Protein Binding; D012680:Sensitivity and Specificity
D052157:Aptamers, Nucleotide; D007073:Immunoglobulin E; D004247:DNA