Molecular characterization of the pina gene in einkorn wheat.
Fifty-six sequences encoding the pina protein were characterized from three species or subspecies of einkorn wheat. These sequences contained 1,595 nucleotides, including 1,270 conserved sites, 21 single nucleotide polymorphisms (SNPs), and 16 indels. The average frequency of SNPs and indels was one out of 76.1 and 99.9 bases, respectively. Five SNPs and no indels were found in the translated sequences. Fourteen haplotypes were defined, and the accessions in each haplotype ranged from 1 to 18. There were nine haplotypes in Triticum monococcum ssp. aegilopoides, eight in T. monococcum ssp. monococcum, and two in T. urartu. Phylogenetic analysis showed that pina genes from different species or subspecies could be clearly differentiated based on the open reading frame. Genes from T. urartu grouped together, whereas genes from T. monococcum ssp. aegilopoides and T. monococcum ssp. monococcum were shared by three and two clusters, respectively. Both the haplotype and phylogenetic analyses indicated that T. monococcum ssp. aegilopoides was more diverse. These results would contribute to the understanding of functional aspects and efficient utilization of pina genes.
Chen
Qing
Q
Triticeae Research Institute, Sichuan Agricultural University, Yaan, Sichuan, China.
Qi
Peng-Fei
PF
Wei
Yu-Ming
YM
Wang
Ji-Rui
JR
Zheng
You-Liang
YL
D000595:Amino Acid Sequence; D018744:DNA, Plant / Q000737:chemistry / Q000235:genetics; D005787:Gene Frequency; D014644:Genetic Variation; D006239:Haplotypes; D054643:INDEL Mutation; D008969:Molecular Sequence Data; D010802:Phylogeny; D010940:Plant Proteins / Q000145:classification / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D014908:Triticum / Q000145:classification / Q000235:genetics*
D018744:DNA, Plant; D010940:Plant Proteins