Brain injury does not alter the intrinsic differentiation potential of adult neuroblasts.
Neuroblasts produced by the neural stem cells of the adult subventricular zone (SVZ) migrate into damaged brain areas after stroke or other brain injuries, and previous data have suggested that they generate regionally appropriate new neurons. To classify the types of neurons produced subsequent to ischemic injury, we combined BrdU or virus labeling with multiple neuronal markers to characterize new cells at different times after the induction of stroke. We show that SVZ neuroblasts give rise almost exclusively to calretinin-expressing cells in the damaged striatum, resulting in the accumulation of these cells during long term recovery after stroke. The vast majority of SVZ neuroblasts as well as newly born young and mature neurons in the damaged striatum constitutively express the transcription factor Sp8, but do not express transcription factors characteristic of medium-sized spiny neurons, the primary striatal projection neurons lost after stroke. Our results suggest that adult neuroblasts do not alter their intrinsic differentiation potential after brain injury.
Liu
Fang
F
Institutes of Brain Science and State Key Laboratory of Medical Neurobiology, Fudan University, 200032 Shanghai, People's Republic of China.
You
Yan
Y
Li
Xiaosu
X
Ma
Tong
T
Nie
Yanzhen
Y
Wei
Bin
B
Li
Tiejun
T
Lin
Huanbing
H
Yang
Zhengang
Z
D000375:Aging / Q000502:physiology*; D000818:Animals; D001930:Brain Injuries / Q000473:pathology* / Q000503:physiopathology; D008297:Male; D055495:Neurogenesis / Q000502:physiology*; D009474:Neurons / Q000166:cytology* / Q000473:pathology / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013234:Stem Cells / Q000166:cytology / Q000473:pathology / Q000502:physiology