Porphyrin-based photocatalytic nanolithography: a new fabrication tool for protein arrays.
Nanoarray fabrication is a multidisciplinary endeavor encompassing materials science, chemical engineering, and biology. We formed nanoarrays via a new technique, porphyrin-based photocatalytic nanolithography. The nanoarrays, with controlled features as small as 200 nm, exhibited regularly ordered patterns and may be appropriate for (a) rapid and parallel proteomics screening of immobilized biomolecules, (b) protein-protein interactions, and/or (c) biophysical and molecular biology studies involving spatially dictated ligand placement. We demonstrated protein immobilization utilizing nanoarrays fabricated via photocatalytic nanolithography on silicon substrates where the immobilized proteins are surrounded by a non-fouling polymer background.
Bearinger
Jane P
JP
Lawrence Livermore National Laboratory, Livermore, California 94550, USA. bearinger1@llnl.gov
Stone
Gary
G
Dugan
Lawrence C
LC
El Dasher
Bassem
B
Stockton
Cheryl
C
Conway
James W
JW
Kuenzler
Tobias
T
Hubbell
Jeffrey A
JA
D002384:Catalysis / Q000528:radiation effects; D055767:Immobilized Proteins / Q000032:analysis* / Q000737:chemistry; D008027:Light; D018625:Microscopy, Atomic Force; D008855:Microscopy, Electron, Scanning; D049329:Nanostructures / Q000737:chemistry / Q000648:ultrastructure; D036103:Nanotechnology / Q000295:instrumentation / Q000379:methods*; D011166:Porphyrins / Q000737:chemistry*; D040081:Protein Array Analysis / Q000295:instrumentation / Q000379:methods; D040901:Proteomics / Q000295:instrumentation / Q000379:methods*; D012825:Silicon / Q000737:chemistry
D055767:Immobilized Proteins; D011166:Porphyrins; D012825:Silicon