Biomolecular-motor-based nano- or microscale particle translocations on DNA microarrays.
We aimed to create autonomous on-chip systems that perform targeted translocations of nano- or microscale particles in parallel using machinery that mimics biological systems. By exploiting biomolecular-motor-based motility and DNA hybridization, we demonstrate that single-stranded DNA-labeled microtubules gliding on kinesin-coated surfaces acted as cargo translocators and that single-stranded DNA-labeled cargoes were loaded/unloaded onto/from gliding microtubules at micropatterned loading/unloading sites specified by DNA base sequences. Our results will help to create autonomous molecular sorters and sensors.
Hiyama
Satoshi
S
Department of Life Sciences, Graduate School of Arts and Sciences, The University of Tokyo, Tokyo 153-8902, Japan. hiyama@nttdocomo.co.jp
Gojo
Riho
R
Shima
Tomohiro
T
Takeuchi
Shoji
S
Sutoh
Kazuo
K
D000818:Animals; D001483:Base Sequence; D004277:DNA, Single-Stranded / Q000737:chemistry*; D016547:Kinesins / Q000737:chemistry*; D008870:Microtubules / Q000737:chemistry*; D018716:Molecular Mimicry; D053758:Nanoparticles / Q000737:chemistry*; D020411:Oligonucleotide Array Sequence Analysis; D010316:Particle Size; D013552:Swine
D004277:DNA, Single-Stranded; D016547:Kinesins