title: Regulation of the formation and trafficking of vesicles from Golgi by PCH family proteins during chemotaxis.
abstract: Previous study demonstrated that WASP localizes on vesicles during Dictyostelium chemotaxis and these vesicles appear to be preferentially distributed at the leading and trailing edge of migrating cells. In this study, we have examined the role of PCH family proteins, Nwk/Bzz1p-like protein (NLP) and Syndapin-like protein (SLP), in the regulation of the formation and trafficking of WASP-vesicles during chemotaxis. NLP and SLP appear to be functionally redundant and deletion of both nlp and slp genes causes the loss of polarized F-actin organization and significant defects in chemotaxis. WASP and NLP are colocalized on vesicles and interactions between two molecules via the SH3 domain of NLP/SLP and the proline-rich repeats of WASP are required for vesicle formation from Golgi. Microtubules are required for polarized trafficking of these vesicles as vesicles showing high directed mobility are absent in cells treated with nocodazole. Our results suggest that interaction of WASP with NLP/SLP is required for the formation and trafficking of vesicles from Golgi to the membrane, which might play a central role in the establishment of cell polarity during chemotaxis.
authors.affiliation: Department of Pharmacology, Vanderbilt University Medical Center, Nashville, TN 37232-6600, USA.
authors.forename: S
authors.initials: S
authors.lastname: Lee
authors.forename: J W
authors.initials: JW
authors.lastname: Han
authors.forename: L
authors.initials: L
authors.lastname: Leeper
authors.forename: J S
authors.initials: JS
authors.lastname: Gruver
authors.forename: C Y
authors.initials: CY
authors.lastname: Chung
mesh_terms: D000199:Actins / Q000378:metabolism; D000818:Animals; D002633:Chemotaxis / Q000502:physiology*; D004023:Dictyostelium / Q000235:genetics / Q000378:metabolism*; D029721:Drosophila Proteins / Q000378:metabolism*; D006056:Golgi Apparatus / Q000378:metabolism*; D008870:Microtubules; D018832:Molecular Chaperones / Q000378:metabolism*; D009687:Nuclear Proteins / Q000378:metabolism*; D056489:Nucleoplasmins; D010750:Phosphoproteins / Q000378:metabolism*; D011392:Proline / Q000737:chemistry / Q000378:metabolism; D021381:Protein Transport; D022142:Secretory Vesicles / Q000378:metabolism*; D014157:Transcription Factors / Q000378:metabolism*; D051316:Wiskott-Aldrich Syndrome Protein / Q000378:metabolism*; D018909:src Homology Domains
chemical_list: D000199:Actins; D029721:Drosophila Proteins; D018832:Molecular Chaperones; C533549:Nlp protein, Drosophila; D009687:Nuclear Proteins; D056489:Nucleoplasmins; D010750:Phosphoproteins; D014157:Transcription Factors; C477859:WASp protein, Drosophila; D051316:Wiskott-Aldrich Syndrome Protein; C074838:slp1 protein, Drosophila; D011392:Proline