Evidence of circulation of Laguna Negra-like hantavirus in the Central West of Brazil: case report.
BACKGROUND
Hantavirus pulmonary syndrome has been reported with increasing frequency in some Brazilian regions, but information about viral genetic identification is still limited. Recently, the state of Mato Grosso, in the Legal Amazon of Brazil, experienced a growing number of hantavirus pulmonary syndrome (HPS) cases but the genetic characterization of the causative hantavirus is still missing.


OBJECTIVE
Our goal was to identify the hantavirus strain involved in a fatal HPS case in the Central region of Brazil.


METHODS
Nested RT-PCR was conducted on blood clot samples from an HPS patient from Mato Grosso. PCR-positive samples were sequenced, and the resulting sequences were compared with reference samples. Viral antigens were detected by immunohistological analyses in lung and liver tissues.


RESULTS
Analyses of the viral RNA isolated from the patient identified a Laguna Negra (LN)-like virus as the causative agent and histological analysis of lung sections were compatible with the genetic characterization.


CONCLUSIONS
This is the first report of circulation and human infection by a Laguna Negra-like hantavirus in Brazil.
Raboni
Sonia Mara
SM
Instituto Carlos Chagas, ICC/Fiocruz/PR, Brazil.
de Borba
Luana
L
Hoffmann
Federico G
FG
de Noronha
Lucia
L
Azevedo
Marin Luize Viola
ML
Carstensen
Suzana
S
Mazzarotto
Giovanny A C A
GA
Bordignon
Juliano
J
dos Santos
Claudia Nunes Duarte
CN
D000328:Adult; D001938:Brazil; D017809:Fatal Outcome; D006476:Orthohantavirus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D018778:Hantavirus Infections / Q000175:diagnosis* / Q000821:virology*; D006801:Humans; D008099:Liver / Q000473:pathology / Q000821:virology; D008168:Lung / Q000473:pathology / Q000821:virology; D008297:Male; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012367:RNA, Viral / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D055815:Young Adult
D012367:RNA, Viral