Chromatin immunoprecipitation using microarrays.
Chromatin immunoprecipitation (ChIP) is a powerful procedure to investigate the interactions between proteins and DNA. ChIP-chip combines chromatin immunoprecipitation and DNA microarray analysis to identify protein-DNA interactions that occur in vivo. This genome-wide analysis of protein-DNA association is carried out in several steps including chemical cross-linking, cell lysis, DNA fragmentation and immunoaffinity purification that allow the identification of DNA interactions and provide a powerful tool for genome-wide investigations. Immunoprecipitated DNA fragments associated with the desired protein are amplified, labelled and hybridized to DNA microarrays to detect enriched signals compared to a labelled reference sample.
Durand-Dubief
Mickaël
M
Karolinska Institute /NOVUM, Huddinge, Sweden.
Ekwall
Karl
K
D002843:Chromatin / Q000378:metabolism; D047369:Chromatin Immunoprecipitation / Q000379:methods*; D005557:Formaldehyde; D008863:Microspheres; D009687:Nuclear Proteins / Q000302:isolation & purification; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D016133:Polymerase Chain Reaction; D015203:Reproducibility of Results; D012568:Schizosaccharomyces / Q000378:metabolism*; D013010:Sonication; D016707:Tissue Fixation
D002843:Chromatin; D009687:Nuclear Proteins; D005557:Formaldehyde