title: Characterization of recombinant human cementum protein 1 (hrCEMP1): primary role in biomineralization.
abstract: Cementum protein 1 (CEMP1) has been recently cloned, and in vitro experiments have shown functions as regulator of cementoblast behavior and inducer of differentiation of non-osteogenic cells toward a cementoblastic/osteoblastic phenotype. In this study, we have produced a full-length human recombinant CEMP1 protein in a human gingival fibroblast cell line. The purified protein (hrCEMP1) has a M(r) 50,000. Characterization of hrCEMP1 indicates that its secondary structure is mainly composed of beta-sheet (55%), where random coil and alpha helix conformations correspond to 35% and 10%, respectively. It was found that hrCEMP1 is N-glycosylated, phosphorylated and possesses strong affinity for hydroxyapatite. Even more important, our results show that hrCEMP1 plays a role during the biomineralization process by promoting octacalcium phosphate (OCP) crystal nucleation. These features make CEMP1 a very good candidate for biotechnological applications in order to achieve cementum and/or bone regeneration.
authors.affiliation: Laboratorio de Biología Periodontal y Tejidos Mineralizados, Facultad de Odontología, UNAM, México DF, Mexico.
authors.forename: Eduardo
authors.initials: E
authors.lastname: Villarreal-Ramírez
authors.forename: Abel
authors.initials: A
authors.lastname: Moreno
authors.forename: Jaime
authors.initials: J
authors.lastname: Mas-Oliva
authors.forename: Juan Luis
authors.initials: JL
authors.lastname: Chávez-Pacheco
authors.forename: A Sampath
authors.initials: AS
authors.lastname: Narayanan
authors.forename: Ivet
authors.initials: I
authors.lastname: Gil-Chavarría
authors.forename: Margarita
authors.initials: M
authors.lastname: Zeichner-David
authors.forename: Higinio
authors.initials: H
authors.lastname: Arzate
mesh_terms: D002113:Calcification, Physiologic; D017886:Durapatite / Q000737:chemistry; D005347:Fibroblasts / Q000378:metabolism; D005881:Gingiva / Q000166:cytology / Q000378:metabolism; D006031:Glycosylation; D006801:Humans; D010766:Phosphorylation; D017433:Protein Structure, Secondary; D011506:Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D011994:Recombinant Proteins / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics
chemical_list: C510886:CEMP1 protein, human; D011506:Proteins; D011994:Recombinant Proteins; D017886:Durapatite