Design of liposome-based pH sensitive nanoSPIN probes: nano-sized particles with incorporated nitroxides.
Liposome-based nanoSized Particles with Incorporated Nitroxides, or nanoSPINs, were designed for EPR applications as pH probes in biological systems. Phospholipid membrane of the liposomes with incorporated gramicidin A showed selective permeability to a small analyte, H(+), while protecting entrapped sensing nitroxide from biological reductants. An application of the pH-sensitive nanoSPIN in an ischemia model in rat heart homogenate allows for monitoring ischemia-induced acidosis while protecting encapsulated nitroxide against bioreduction.
Woldman
Yakov Y
YY
The Ohio State University Medical Center, Columbus, 43210, USA.
Semenov
Sergey V
SV
Bobko
Andrey A
AA
Kirilyuk
Igor A
IA
Polienko
Julya F
JF
Voinov
Maxim A
MA
Bagryanskaya
Elena G
EG
Khramtsov
Valery V
VV
D000138:Acidosis; D000818:Animals; D055664:Electrochemical Techniques; D004578:Electron Spin Resonance Spectroscopy / Q000379:methods; D006096:Gramicidin / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D007511:Ischemia; D008081:Liposomes / Q000737:chemistry*; D015394:Molecular Structure; D009206:Myocardium / Q000378:metabolism; D053758:Nanoparticles / Q000737:chemistry*; D009589:Nitrogen Oxides / Q000737:chemistry*; D010101:Oxygen Consumption; D051381:Rats; D017207:Rats, Sprague-Dawley
D008081:Liposomes; D009589:Nitrogen Oxides; D006096:Gramicidin