[Identification of a novel HLA allele DRB1*1462 by sequence-based typing].
This study was to identify a novel HLA-DRB1 allele in Chinese population by nucleotide sequence ana- lysis. The HLA typing of genes was performed by PCR-SSO and PCR-SSP, the ambiguous novel allele was identified by DNA sequence analysis. The results showed that the sequence of this new allele differed from DRB1*140101 by one nucleotide substitution at position 256 in exon 2 (G- > A), resulting in an amino acid change from Ala to Thr at codon 57. In conclusion, this allele is a novel one, which has been officially given the name DRB1*1462 by the WHO nomenclature committee in January 2006.
Cui
Shuang
S
HLA Laboratory, Beijing Red Cross Blood Center, Beijing 100088, China.
Shan
Xiao-Yan
XY
He
Xiao-Mei
XM
Liu
Na
N
Wang
Lin
L
Li
Wei
W
Wang
Li-Jun
LJ
Ni
Lei
L
Wang
Dong-Mei
DM
Zhao
Bo-Tao
BT
Gong
Zhi-Yin
ZY
Zhang
Zhi-Xin
ZX
D000483:Alleles; D044466:Asian People / Q000235:genetics; D006684:HLA-DR Antigens / Q000235:genetics*; D059811:HLA-DRB1 Chains; D006650:Histocompatibility Testing; D006801:Humans; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA
D006684:HLA-DR Antigens; D059811:HLA-DRB1 Chains