Simultaneous quantification of 19 ginsenosides in black ginseng developed from Panax ginseng by HPLC-ELSD.
A high-performance liquid chromatographic method with evaporative light scattering detection (HPLC-ELSD) has been developed to identify and quantify 19 ginsenosides (Rg(1), Re, Rf, Rb(1), Rc, Rb(2), Rd, F(4), Rg(6), Rk(3), Rh(4), 20(S)-, 20(R)-Rg(3), 20(S)-, 20(R)-Rs(3), Rk(1), Rg(5), Rs(4), and Rs(5)) in black ginseng (BG, Korean white ginseng that was subjected to nine cycles of steam treatment). Ultrasonication is employed for sample preparation, and the analysis is achieved on a Discovery C(18) column using gradient elution of CH(3)CN-H(2)O-CH(3)COOH without buffer in 40min. The method was validated by linearity (r(2)> or =0.9994), precision (92.0-107.5%), intra- and inter-day accuracy (R.S.D.<3.21%), and limit of detection (LOD< or =93ng). The quantification method was applied to analyze the composition of ginsenosides in Korean white, red, and black ginsengs. During the preparatory process of BG, ginsenosides transform into constituents of low polarity by hydrolysis, isomerization, and dehydration at C-20, and hydrolysis also occurs at C-3 or C-6. The validated HPLC method is expected to provide the basis for the quality assessment of ginseng products.
Sun
Bai-Shen
BS
Department of Food Science and Technology, Chungnam National University, Yusung-Gu, Taejeon, South Korea.
Gu
Li-Juan
LJ
Fang
Zhe-Ming
ZM
Wang
Chun-yan
CY
Wang
Zhen
Z
Lee
Mi-Ra
MR
Li
Zheng
Z
Li
Jing-Jie
JJ
Sung
Chang-Keun
CK
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D036145:Ginsenosides / Q000032:analysis*; D005894:Panax / Q000737:chemistry*; D015203:Reproducibility of Results
D036145:Ginsenosides