title: Mercury(II) and methyl mercury speciation on Streptococcus pyogenes loaded Dowex Optipore SD-2.
abstract: A solid phase extraction procedure based on speciation of mercury(II) and methyl mercury on Streptococcus pyogenes immobilized on Dowex Optipore SD-2 has been established. Selective and sequential elution with 0.1 mol L(-1) HCl for methyl mercury and 2 mol L(-1) HCl for mercury(II) were performed at pH 8. The determination of mercury levels was performed by cold vapour atomic absorption spectrometry (CVAAS). Optimal analytical conditions including pH, amounts of biosorbent, sample volumes, etc., were investigated. The influences of the some alkaline and earth alkaline ions and some transition metals on the recoveries were also investigated. The capacity of biosorbent for mercury(II) and methyl mercury was 4.8 and 3.4 mg g(-1). The detection limit (3 sigma) of the reagent blank for mercury(II) and methyl mercury was 2.1 and 1.5 ng L(-1). Preconcentration factor was calculated as 25. The relative standard deviations of the procedure were below 7%. The validation of the presented procedure is performed by the analysis of standard reference material (NRCC-DORM 2 Dogfish Muscle). The procedure was successfully applied to the speciation of mercury(II) and methyl mercury in natural water and environmental samples.
authors.affiliation: Chemistry Department, Faculty of Science and Arts, Gaziosmanpaşa University, 60250 Tokat, Turkey. m.tuzen@gmail.com
authors.forename: Mustafa
authors.initials: M
authors.lastname: Tuzen
authors.forename: Ozgur Dogan
authors.initials: OD
authors.lastname: Uluozlu
authors.forename: Isa
authors.initials: I
authors.lastname: Karaman
authors.forename: Mustafa
authors.initials: M
authors.lastname: Soylak
mesh_terms: D000327:Adsorption; D001673:Biodegradation, Environmental; D005618:Fresh Water / Q000737:chemistry; D008628:Mercury / Q000302:isolation & purification*; D008767:Methylmercury Compounds / Q000302:isolation & purification*; D012015:Reference Standards; D012117:Resins, Synthetic; D052616:Solid Phase Extraction / Q000379:methods*; D013054:Spectrophotometry, Atomic / Q000379:methods; D013297:Streptococcus pyogenes / Q000737:chemistry*; D018508:Water Purification / Q000379:methods
chemical_list: D008767:Methylmercury Compounds; D012117:Resins, Synthetic; C027972:Dowex; D008628:Mercury