Entrainment of Drosophila circadian clock to green and yellow light by Rh1, Rh5, Rh6 and CRY.
Light is one of the most important time cues for entrainment of the circadian clock. Drosophila circadian photoreception is mediated by cryptochrome in clock neurons and by rhodopsins in photic organs. We generated Rh5 mutants to elucidate circadian photoreception by rhodopsins. The Rh1, Rh5 and Rh6 mutants were combined with cry, and entrained to a 6-h delayed photoperiod. The cry, Rh1, Rh5 and Rh6 quadruple mutant became entrained by white light. In contrast, reentrainment to green and yellow light was abolished in the cry, Rh1, Rh5 and Rh6 quadruple mutant, and remarkably slowed in the cry, Rh1 and Rh6 triple mutant. These results suggest that cry, Rh1, Rh5 and Rh6 are essential for circadian photoentrainment to green and yellow light.
Hanai
Shuji
S
Clock Cell Biology Research Group, National Institute of Advanced Industrial Science and Technology, Higashi, Japan.
Ishida
Norio
N
D000818:Animals; D001683:Biological Clocks / Q000502:physiology* / Q000528:radiation effects; D002940:Circadian Rhythm / Q000502:physiology* / Q000528:radiation effects; D056931:Cryptochromes; D004330:Drosophila / Q000235:genetics / Q000378:metabolism*; D029721:Drosophila Proteins / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D005136:Eye Proteins / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D008027:Light; D009154:Mutation / Q000235:genetics; D010775:Photic Stimulation; D017440:Photoperiod; D043562:Receptors, G-Protein-Coupled / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D025603:Sensory Rhodopsins / Q000235:genetics / Q000378:metabolism* / Q000528:radiation effects
D056931:Cryptochromes; D029721:Drosophila Proteins; D005136:Eye Proteins; D043562:Receptors, G-Protein-Coupled; D025603:Sensory Rhodopsins; C119828:cry protein, Drosophila