title: Gene expression profiling of resistant and susceptible Bombyx mori strains reveals nucleopolyhedrovirus-associated variations in host gene transcript levels.
abstract: We investigated variations in the gene expression of Bombyx mori following infection with a nucleopolyhedrovirus (BmNPV). Two B. mori strains, KN and 306, which are highly resistant and susceptible to BmNPV infection, respectively, were used in this study. The infection profiles of BmNPV in the B. mori KN and 306 larvae revealed that the virus invaded the midguts of both these strains. However, its proliferation was notably inhibited in the midgut of the resistant strain. By using the suppression subtractive hybridization method, two cDNA libraries were constructed in order to compare the BmNPV responsive gene expressions between the two silkworm lines. In total, 62 differentially expressed genes were obtained. Real-time qPCR analysis confirmed that eight genes were significantly up-regulated in the midgut of the KN strain following BmNPV infection. Our results imply that these up-regulated genes may be involved in the B. mori immune response against BmNPV infection.
authors.affiliation: Key Laboratory of Molecular Biology of Crop Pathogens and Insects, Ministry of Agriculture, Institute of Insect Sciences, Zhejiang University, Hangzhou, 310029, China.
authors.forename: Yan-Yuan
authors.initials: YY
authors.lastname: Bao
authors.forename: Xu-Dong
authors.initials: XD
authors.lastname: Tang
authors.forename: Zu-Yao
authors.initials: ZY
authors.lastname: Lv
authors.forename: Xiu-Ye
authors.initials: XY
authors.lastname: Wang
authors.forename: Cai-Hong
authors.initials: CH
authors.lastname: Tian
authors.forename: Yi Peng
authors.initials: YP
authors.lastname: Xu
authors.forename: Chuan-Xi
authors.initials: CX
authors.lastname: Zhang
mesh_terms: D000818:Animals; D001483:Base Sequence; D012831:Bombyx / Q000235:genetics* / Q000648:ultrastructure / Q000821:virology*; D018076:DNA, Complementary / Q000235:genetics / Q000302:isolation & purification; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D014644:Genetic Variation; D007814:Larva / Q000648:ultrastructure / Q000821:virology; D046529:Microscopy, Electron, Transmission; D017924:Nucleopolyhedroviruses / Q000235:genetics*; D016133:Polymerase Chain Reaction; D014158:Transcription, Genetic
chemical_list: D018076:DNA, Complementary