title: Pediococcus lolii sp. nov., isolated from ryegrass silage.
abstract: A Gram-positive, coccus-shaped, lactic acid bacterium, strain NGRI 0510Q(T), was isolated from ryegrass silage produced in Okinawa Prefecture, Japan. The cell is non-spore-forming, non-motile, and occurs in pairs or tetrads. The strain is homofermentative and produces d- and l-lactic acid from glucose. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain NGRI 0510Q(T) belongs to the genus Pediococcus and clusters within the Pediococcus acidilactici and Pediococcus pentosaceus group, with 98.2 and 96.9 % sequence identity, respectively. DNA-DNA relatedness between strain NGRI 0510Q(T) and P. acidilactici JCM 8797(T) and P. pentosaceus JCM 5890(T) was 19.3 and 17.3 %, respectively. Based on its phenotypic characteristics, phylogenetic relationship and DNA-DNA relatedness, NGRI 0510Q(T) (=JCM 15055(T)=DSM 19927(T)) represents the type strain of a novel species, for which the name Pediococcus lolii sp. nov. is proposed.
authors.affiliation: Department of Genetic Resources Technology, Faculty of Agriculture, Kyushu University, 6-10-1 Hakozaki, Higashi-ku, Fukuoka 812-8581, Japan. doi@agr.kyushu-u.ac.jp
authors.forename: Katsumi
authors.initials: K
authors.lastname: Doi
authors.forename: Yousuke
authors.initials: Y
authors.lastname: Nishizaki
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Fujino
authors.forename: Toshihisa
authors.initials: T
authors.lastname: Ohshima
authors.forename: Sadahiro
authors.initials: S
authors.lastname: Ohmomo
authors.forename: Seiya
authors.initials: S
authors.lastname: Ogata
mesh_terms: D015373:Bacterial Typing Techniques; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics; D020459:Genes, rRNA; D007564:Japan; D008129:Lolium / Q000382:microbiology*; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010376:Pediococcus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology; D010641:Phenotype; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012820:Silage / Q000382:microbiology*; D013045:Species Specificity
chemical_list: D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S