A new micro/nanoencapsulated porphyrin formulation for PDT treatment.
The highly hydrophobic 5,10,15-triphenyl-20-(3-N-methylpyridinium-yl)porphyrin (3MMe) cationic species was synthesized, characterized and encapsulated in marine atelocollagen/xanthane gum microcapsules by the coacervation method. Further reduction in the capsule size, from several microns down to about 300-400 nm, was carried out successfully by ultrasonic processing in the presence of up to 1.6% Tween 20 surfactant, without affecting the distribution of 3MMe in the oily core. The resulting cream-like product exhibited enhanced photodynamic activity but negligible cytotoxicity towards HeLa cells. The polymeric micro/nanocapsule formulation was found to be about 4 times more phototoxic than the respective phosphatidylcholine lipidic emulsion, demonstrating high potentiality for photodynamic therapy applications.
Deda
Daiana K
DK
Institute of Chemistry, University of Sao Paulo, Sao Paulo, SP, Brazil.
Uchoa
Adjaci F
AF
Caritá
Eduardo
E
Baptista
Maurício S
MS
Toma
Henrique E
HE
Araki
Koiti
K
D002214:Capsules / Q000138:chemical synthesis / Q000737:chemistry*; D002470:Cell Survival / Q000187:drug effects; D004337:Drug Carriers / Q000138:chemical synthesis*; D016503:Drug Delivery Systems / Q000379:methods*; D006367:HeLa Cells; D006801:Humans; D053769:Nanocapsules / Q000737:chemistry*; D010778:Photochemotherapy / Q000379:methods*; D011166:Porphyrins / Q000008:administration & dosage* / Q000138:chemical synthesis / Q000494:pharmacology
D002214:Capsules; D004337:Drug Carriers; D053769:Nanocapsules; D011166:Porphyrins