Detection of TT virus by single-primer sequence-independent amplification in multiple samples collected from an outbreak of gastroenteritis.
A panel of gastroenteritis outbreak samples was subjected to a virus purification and concentration algorithm followed by a sequence-independent amplification method devised to detect viral enteric pathogens. The application of these methods allowed the identification of torque teno virus (TTV) in one outbreak. The complete genome sequence of 3,260 nt was obtained through "genome walking", and four open reading frames were deduced from the genomic sequence. Phylogenetic analysis grouped this virus in TTV genetic group 3, clustering with genotype 27, with 85% similarity at the nt level with strain SAa-01.
Braham
S
S
Enteric Virus Unit, Virus Reference Department, Centre for Infections, Health Protection Agency, Colindale, London, NW9 5HT, UK.
Iturriza-Gómara
M
M
Gray
J
J
D017931:DNA Primers / Q000235:genetics*; D004266:DNA Virus Infections / Q000175:diagnosis* / Q000453:epidemiology* / Q000821:virology; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics; D004196:Disease Outbreaks; D005759:Gastroenteritis / Q000453:epidemiology* / Q000821:virology*; D016679:Genome, Viral; D006801:Humans; D008969:Molecular Sequence Data; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D016366:Open Reading Frames; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D022783:Torque teno virus / Q000235:genetics / Q000302:isolation & purification*
D017931:DNA Primers; D004279:DNA, Viral