[An experimental luminescent immunochromatographic system].
Luminescent immunochromatographic assay for antigens of microorganisms was developed to detect antigens and cells of microorganisms, toxins. Luminescent submicrone latex particles, quantum labels, ganglioside-containing liposomes with sulforhodamine-01 in the internal volume were used as a luminescent mobile phase for conjugation with antibodies. Devices for registration of luminescent immunochromatograms were developed. Submicrone latexes were shown to have advantages over quantum labels, ganglioside-containing liposomes with sulforhodamine-101 and colloid gold for immunochromatographic procedures. Sensitivity of luminescent latex-based immunochromatographic indicator element was 3-30 that elements based on colloid gold conjugates for identical microorganisms and elements.
Iarkov
S P
SP
Skopinskaia
S N
SN
Shilenko
I V
IV
Zlobin
V N
VN
D000942:Antigens, Bacterial / Q000032:analysis*; D001419:Bacteria / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D001427:Bacterial Toxins / Q000032:analysis*; D002845:Chromatography / Q000295:instrumentation*; D006801:Humans; D008163:Luminescent Measurements / Q000295:instrumentation*; D015394:Molecular Structure; D012680:Sensitivity and Specificity
D000942:Antigens, Bacterial; D001427:Bacterial Toxins