Cytokine synthesis by chondroblastoma: relation to local inflammation.
OBJECTIVE
To investigate cytokine production by chondroblastoma in inducing local inflammation and adjacent-joint arthritis.


METHODS
Immunohistochemical analyses of curetted tissues using anti-human interleukin (IL)-1 beta, IL- 6, IL-8, and tumour necrosis factor (TNF)-alpha were performed for 6 patients with chondroblastoma and 3 patients with giant cell tumour (GCT) of bone. In addition, prostaglandin E2, IL-1 beta, IL-2, IL-4, IL-5, IL-6, IL-8, and TNF-alpha in the cyst fluid of one of the patients with chondroblastoma and 2 with GCT of bone were measured using immunoassay kits.


RESULTS
More positive staining for IL-1 beta, IL-8, IL- 6, and TNF-alpha was shown in chondroblastoma than GCT of bone samples. Osteoclast-like giant cells in chondroblastomas showed positive staining for IL- 6 only. In addition, concentrations of IL-4, IL-6, and IL-8 in the cyst fluid were higher in the one patient with chondroblastoma than the 2 patients with GCT of bone.


CONCLUSIONS
Cytokines such as IL-1 beta, IL-8, TNF-alpha, and particularly IL-6 play an important role in local inflammation in patients with chondroblastoma.
Uchikawa
C
C
Department of Orthopaedic Surgery, Gunma University Graduate School of Medicine, Maebashi, Gunma, Japan.
Shinozaki
T
T
Nakajima
T
T
Takagishi
K
K
D000293:Adolescent; D001168:Arthritis / Q000209:etiology / Q000378:metabolism* / Q000473:pathology; D016022:Case-Control Studies; D002804:Chondroblastoma / Q000378:metabolism* / Q000473:pathology; D015331:Cohort Studies; D016207:Cytokines / Q000378:metabolism*; D005260:Female; D005266:Femoral Neoplasms / Q000378:metabolism* / Q000473:pathology; D005270:Femur Head; D018212:Giant Cell Tumor of Bone / Q000378:metabolism* / Q000473:pathology; D006621:Hip Joint; D006801:Humans; D007719:Knee Joint; D008297:Male; D013977:Tibia; D055815:Young Adult
D016207:Cytokines