The another toxic effect of carbamate insecticides.
The activities of the antioxidant and detoxifying enzymes, superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSHPx), glutathione reductase (GR), glutathione-S-transferase (GST), and the content of thiobarbituric acid reactive substances (TBARS) were determined in the liver and kidney of rabbits after exposure to bendiocarb. In the liver, the activities of SOD, CAT and GR were not affected by bendiocarb. The induction or inhibition of isoenzymes of SOD (mainly MnSOD) were observed in the experimental groups. The activities of GSHPx-cum and GSHPx-H2O2 significantly decreased on the days 3 and 10 of the experiment. The activity of GST significantly increased on the day 9 of the experiment. In the kidney, the activity of SOD was significantly increased and the new MnSOD isoenzymes were detected. The activities of CAT and GSHPx-H2O2 were significantly decreased in the experimental groups. The activity of GR significantly increased on days 3 and 10, and the activity of GST was significantly increased on days 3, 10, and 30. Exposure of rabbit to bendiocarb did not affect the content of TBARS in the kidney. In the liver, the content of TBARS was significantly increased in the experimental groups as compared to the control. Our results showed that the response of organs to bendiocarb is different and may depend on the specific organ damage and their protective abilities. The alterations in the activities of the antioxidant defence system, increased TBARS values, and changes in the SOD isoenzyme pattern showed that the toxic effect of bendiocarb is not only in the acetylcholine esterase inhibition, but also in ROS production.
Sobeková
Anna
A
Department of Chemistry, Biochemistry and Biophysics, Histology and Physiology University of Veterinary Medicine, Komenského 73, 041 81 Kosice, Slovak Republic. asobekova@hotmail.com
Holovská
Katarína
K
Lenártová
Viera
V
Flesárová
Slávka
S
Javorský
P
P
D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D007306:Insecticides / Q000633:toxicity*; D007668:Kidney / Q000187:drug effects* / Q000201:enzymology; D008099:Liver / Q000187:drug effects* / Q000201:enzymology; D008297:Male; D048448:Phenylcarbamates / Q000633:toxicity*; D011817:Rabbits
D000975:Antioxidants; D007306:Insecticides; D048448:Phenylcarbamates; C007725:bendiocarb