Immunohistochemical localization of phosphohistidine phosphatase PHPT1 in mouse and human tissues.
Protein histidine phosphorylation accounts for about 6% of the total protein phosphorylation in eukaryotic cells; still details concerning histidine phosphorylation and dephosphorylation are limited. A mammalian 14-kDa phosphohistidine phosphatase, also denominated PHPT1, was found 6 years ago that provided a new tool in the study of phosphohistidine phosphorylation. The localization of PHPT1 mRNA by Northern blot analysis revealed high expression in heart and skeletal muscle. The main object of the present study was to determine the PHPT1 expression on protein level in mouse tissues in order to get further information on the physiological role of the enzyme. Tissue samples from adult mice and 14.5-day-old mouse embryos were processed for immunostaining using a PHPT1-specific polyclonal antibody. The same antibody was also provided to the Swedish human protein atlas project (HPR) (http://www.proteinatlas.org/index.php). The results from both studies were essentially consistent with the previously reported expression of mRNA of a few human tissues. In addition, several other tissues, including testis, displayed a high protein expression. A salient result of the present investigation was the ubiquitous expression of the PHPT1 protein and its high expression in continuously dividing epithelial cells.
Zhang
Xiau-Qun
XQ
Department of Medical Biochemistry and Microbiology, Uppsala University, Uppsala, Sweden.
Sundh
Ulla Beckman
UB
Jansson
Leif
L
Zetterqvist
Orjan
O
Ek
Pia
P
D000818:Animals; D000918:Antibody Specificity; D015152:Blotting, Northern; D006801:Humans; D007150:Immunohistochemistry; D051379:Mice; D008810:Mice, Inbred C57BL; D010744:Phosphoric Monoester Hydrolases / Q000235:genetics / Q000378:metabolism*; D010766:Phosphorylation; D013045:Species Specificity
C504019:PHPT1 protein, human; C542172:PHPT1 protein, mouse; D010744:Phosphoric Monoester Hydrolases