title: Optimization of medium composition for butyric acid production by Clostridium thermobutyricum using response surface methodology.
abstract: The optimal medium for butyric acid production by Clostridium thermobutyricum in a shake flask culture was studied using statistical experimental design and analysis. The optimal composition of the fermentation medium for maximum butyric acid yield, as determined on the basis of a three-level four-factor Box-Behnken design (BBD), was obtained by response surface methodology (RSM). The high correlation between the predicted and observed values indicated the validity of the model. A maximum butyric acid yield of 12.05 g/l was obtained at K(2)HPO(4) 7.2 g/l, 34.9 g/l glucose, 20 g/l yeast extract, and 15 g/l acetate, which compared well to the predicated production of 12.13 g/l.
authors.affiliation: Key Laboratory of Biofuel, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Songling road 189, Qingdao 266071, China.
authors.forename: Chun-Hui
authors.initials: CH
authors.lastname: Zhang
authors.forename: Yu-Jiu
authors.initials: YJ
authors.lastname: Ma
authors.forename: Fang-Xiao
authors.initials: FX
authors.lastname: Yang
authors.forename: Wei
authors.initials: W
authors.lastname: Liu
authors.forename: Yue-Dong
authors.initials: YD
authors.lastname: Zhang
mesh_terms: D020148:Butyric Acid / Q000378:metabolism*; D003013:Clostridium / Q000378:metabolism*; D003470:Culture Media; D012984:Software
chemical_list: D003470:Culture Media; D020148:Butyric Acid