title: Point-of-care fluorescence immunoassay for prostate specific antigen.
abstract: BACKGROUND
  Prostate specific antigen (PSA) is widely used as a clinical marker for diagnosis, screening, and prognosis of prostate cancer. A fluorescence (FL) dye-incorporated immunochromatographic assay (ICA) was developed for detection of PSA concentration in whole blood or serum.
  
  
  METHODS
  Whole blood or serum mixed with FL-conjugated detector was loaded onto a cartridge and incubated for 12 min. The FL intensity of the cartridge was then measured in a laser FL scanner. The analytical performance of the FL-ICA system was evaluated by precision and recovery tests. The comparability of the new method was examined with an automated analyzer.
  
  
  RESULTS
  A reliable correlation between area ratio (AT/AC), reflecting FL intensity of test/control line, and PSA concentration was observed (r=0.998). The CVs of intra- and inter-assay precision in a range of 2.5-8 microg/l were <6.0% and 4.5%, respectively, and analytical recovery of the FL-ICA system fell within 6.5% at the tested samples. When the FL-ICA method was compared with Abbott AxSYM and Bayer Centaur analyzers, there were strong correlations (r=0.993 and r=0.992, p<0.0001).
  
  
  CONCLUSIONS
  The FL-ICA system with point-of-care-testing (POCT) appeared to be an easy, fast and suitable method for measurement of PSA concentration in whole blood, and needs no accessory equipment to separate serum.
authors.affiliation: Department of Biology Education, Institute of Fusion Science, Institute of Science Education, Chonbuk National University, Jeonju, 561-756, South Korea.
authors.forename: Sang Wook
authors.initials: SW
authors.lastname: Oh
authors.forename: Young Min
authors.initials: YM
authors.lastname: Kim
authors.forename: Hyun Jeong
authors.initials: HJ
authors.lastname: Kim
authors.forename: Sung Joong
authors.initials: SJ
authors.lastname: Kim
authors.forename: Jin-Sun
authors.initials: JS
authors.lastname: Cho
authors.forename: Eui Yul
authors.initials: EY
authors.lastname: Choi
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000911:Antibodies, Monoclonal / Q000276:immunology; D003101:Collodion; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000032:analysis; D006801:Humans; D007118:Immunoassay / Q000295:instrumentation / Q000379:methods*; D008297:Male; D008567:Membranes, Artificial; D008875:Middle Aged; D019095:Point-of-Care Systems; D017430:Prostate-Specific Antigen / Q000097:blood* / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal; D005456:Fluorescent Dyes; D008567:Membranes, Artificial; D003101:Collodion; D017430:Prostate-Specific Antigen