Placental steroidogenesis in rats is independent of signaling pathways induced by retinoic acids.
We investigated the effects of retinoic acids (RAs) on steroid hormone production and mRNA expression of steroidogenic enzymes in rat placenta in vitro and in vivo. In the rat trophoblast giant cell line Rcho-1, the natural retinoid X receptor (RXR) agonist 9-cis retinoic acid (9cRA) and synthetic RXR agonist LG100268 slightly promoted production of progesterone and androgen, whereas the natural retinoic acid receptor (RAR) agonist all-trans retinoic acid (atRA) and synthetic RAR agonist TTNPB did not. Furthermore, although administration of atRA and 9cRA into the rat uterus at 13.5days postcoitum robustly induced mRNA expression of cellular retinol binding protein II, the gene for which is targeted by RAR and/or RXR, in the placenta, neither RA affected the expression of placental steroidogenic enzymes, and both had little effect on progesterone and androgen levels in the placenta and embryo, suggesting that placental steroidogenesis is not regulated by RAs in rats.
Itoh
Kenji
K
Department of Toxicology, Graduate School of Pharmaceutical Sciences, Osaka University, 1-6 Yamadaoka, Suita, Osaka 565-0871, Japan.
Hiromori
Youhei
Y
Kato
Naoko
N
Yoshida
Ichiro
I
Itoh
Norio
N
Ike
Michihiko
M
Nagase
Hisamitsu
H
Tanaka
Keiichi
K
Nakanishi
Tsuyoshi
T
D000077556:Alitretinoin; D000728:Androgens / Q000378:metabolism; D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology; D001565:Benzoates / Q000494:pharmacology; D002460:Cell Line; D002853:Chromatography, Liquid; D005260:Female; D009539:Nicotinic Acids / Q000494:pharmacology; D010920:Placenta / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism*; D011247:Pregnancy; D011374:Progesterone / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D047488:Retinoid X Receptors / Q000819:agonists / Q000378:metabolism; D012176:Retinoids / Q000494:pharmacology; D054840:Retinol-Binding Proteins, Cellular / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction / Q000187:drug effects*; D021241:Spectrometry, Mass, Electrospray Ionization; D053719:Tandem Mass Spectrometry; D013764:Tetrahydronaphthalenes / Q000494:pharmacology; D014212:Tretinoin / Q000494:pharmacology*
D000728:Androgens; D000970:Antineoplastic Agents; D001565:Benzoates; D009539:Nicotinic Acids; D047488:Retinoid X Receptors; D012176:Retinoids; D054840:Retinol-Binding Proteins, Cellular; D013764:Tetrahydronaphthalenes; D000077556:Alitretinoin; D011374:Progesterone; D014212:Tretinoin; C038864:4-(2-(5,6,7,8-tetrahydro-5,5,8,8-tetramethyl-2-naphthalenyl)-1-propenyl)benzoic acid; C095104:LG 100268