Sleep deprivation attenuates inflammatory responses and ischemic cell death.
Although the biological function of sleep remains uncertain, the consequences of sleep deprivation are well-described and are reported to be detrimental to cognitive function and affective well-being. Sleep deprivation also is strongly associated with elevated risk factors for cardiovascular disease. We used a mouse model of cardiac arrest/cardiopulmonary resuscitation to test the hypothesis that acute sleep deprivation would exacerbate neuroinflammation and neurodegeneration after global ischemia. The resulting data led to a rejection of our hypothesis that sleep deprivation is necessarily detrimental. Indeed, acute sleep deprivation (ASD) was associated with a reduction in ischemia-induced interleukin 1beta (IL-1beta) gene expression and attenuation of neuronal damage in the hippocampus. Further, sleep deprivation increased gene expression of two anti-inflammatory cytokines, IL-6 and IL-10 that are associated with improved ischemic outcome. To determine whether the anti-inflammatory properties of ASD were specific to ischemia, mice were treated systemically with lipopolysaccharide (LPS), a potent inflammogen. Acute sleep deprivation attenuated the central and peripheral increase in tumor necrosis factor-alpha (TNFalpha) and increased IL-10 expression. Together, the ischemia and LPS data suggest that, ASD produces an anti-inflammatory bias that could be exploited to improve medical procedures that are compromised by inflammation.
Weil
Zachary M
ZM
Department of Psychology, and Institute for Behavioral Medicine Research. The Ohio State University, Columbus, OH 43210, USA. zweil@rockefeller.edu
Norman
Greg J
GJ
Karelina
Kate
K
Morris
John S
JS
Barker
Jacqueline M
JM
Su
Alan J
AJ
Walton
James C
JC
Bohinc
Steven
S
Nelson
Randy J
RJ
DeVries
A Courtney
AC
D000704:Analysis of Variance; D000818:Animals; D016887:Cardiopulmonary Resuscitation / Q000379:methods; D016923:Cell Death / Q000502:physiology; D016207:Cytokines / Q000235:genetics / Q000378:metabolism; D004195:Disease Models, Animal; D005452:Fluoresceins; D005786:Gene Expression Regulation / Q000502:physiology; D006323:Heart Arrest / Q000150:complications; D006624:Hippocampus / Q000473:pathology; D007249:Inflammation / Q000209:etiology* / Q000473:pathology / Q000517:prevention & control*; D053583:Interleukin-1beta / Q000235:genetics / Q000378:metabolism; D007511:Ischemia / Q000150:complications* / Q000209:etiology; D008070:Lipopolysaccharides / Q000633:toxicity; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D009930:Organic Chemicals; D011863:Radioimmunoassay / Q000379:methods; D012892:Sleep Deprivation; D013997:Time Factors
D016207:Cytokines; D005452:Fluoresceins; D053583:Interleukin-1beta; D008070:Lipopolysaccharides; D009930:Organic Chemicals; C435731:fluoro jade