Genome analysis and development of infectious cDNA clone of a virulence-attenuated strain of foot-and-mouth disease virus type Asia 1 from China.
The RNA genome sequence of the rabbit passage-attenuated strain of foot-and-mouth disease virus (FMDV) Asia 1, ZB/CHA/58(att), was determined to be 8165 nt in length excluding the poly(C) tract in the 5' UTR and the poly(A) tail at the 3' end. ZB/CHA/58(att) was most similar to the vaccine strain Asia 1/YNBS/58 in genome sequence and there were no deletions or insertions within the deduced polyprotein between ZB/CHA/58(att) and YNBS/58, but there were a total of 25 substitutions at the amino acid level and an extra 19-nt stretch in the 5' UTR was found in ZB/CHA/58(att). An infectious full-length cDNA clone of ZB/CHA/58(att) was developed. Infectious virus could be recovered in BHK-21 cells transfected with the synthetic viral RNA transcribed in vitro. The plaque morphology, growth kinetics and antigenic profile of the infectious clone-derived virus (termed tZB) were indistinguishable from those induced by the parental virus. Furthermore, the virulence properties of ZB/CHA/58(att) and tZB were found to be highly similar in the mouse model. The availability of genome sequence information and infectious cDNA clone of the FMDV ZB/CHA/58(att) lays a new ground for further investigation of FMDV virulence determinants and development of new potent vaccine to FMD.
Xin
Aiguo
A
Guangxi University, Nanning, PR China.
Li
Huachun
H
Li
Le
L
Liao
Defang
D
Yang
Yongqin
Y
Zhang
Nianzu
N
Chen
Baoshan
B
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002417:Cattle; D002460:Cell Line; D002681:China / Q000453:epidemiology; D003001:Cloning, Molecular; D006224:Cricetinae; D018076:DNA, Complementary / Q000235:genetics*; D004279:DNA, Viral / Q000235:genetics*; D005536:Foot-and-Mouth Disease / Q000453:epidemiology / Q000821:virology; D029544:Foot-and-Mouth Disease Virus / Q000145:classification / Q000472:pathogenicity*; D005814:Genes, Viral; D016679:Genome, Viral; D010802:Phylogeny; D014764:Viral Proteins / Q000737:chemistry; D014774:Virulence
D018076:DNA, Complementary; D004279:DNA, Viral; D014764:Viral Proteins