[Enzyme immunoassay for the determination of the glycopeptide antibiotic eremomycin].
An indirect competitive enzyme-linked immunosorbent assay (ELISA) was developed using rabbit polyclonal antibodies against the eremomycin-glucose oxidase conjugated antigen. This technique allows the glycopeptide antibiotic eremomycin to be determined both in aqueous solutions (with a sensitivity as high as 0.1 ng/ml) and in blood plasma. The cross-reactivity of the antibodies with vancomycin was 0.4% of that for eremomycin, while teicoplanin was almost not recognized. Experiments with blood plasma samples diluted 1:10 showed that the assay was linear over the concentration range 1-30 ng/ml and that the variation coefficient did not exceed 10%. The high sensitivity and selectivity of this test make it suitable for pharmacokinetic studies and drug monitoring analysis.
Burkin
M A
MA
Burkin
A A
AA
D000818:Animals; D000906:Antibodies / Q000737:chemistry* / Q000276:immunology; D003429:Cross Reactions / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D006020:Glycopeptides / Q000032:analysis* / Q000276:immunology; D011817:Rabbits; D012680:Sensitivity and Specificity; D014640:Vancomycin / Q000032:analysis / Q000276:immunology
D000906:Antibodies; D006020:Glycopeptides; C053759:eremomycin; D014640:Vancomycin