[Comparison of migration characteristics of MSCs in different assay systems].
The aim of this study was to explore the difference of MSC migration mediated by SDF-1/CXCR4 axis through Boyden chamber in vitro migration assay. The SDF-1 density-dependence of MSC migration was observed. Subsequently, the effects of different blocking agents on hSDF-MSC migration were observed after MSC were treated with 50 nmol/L wortmannin, 10 micromol/L LY294002, 50 micromol/L PD98059, 10 micromol/L U73122, 126 micromol/L AMD3100 and 50 nmol/L verapamil respectively. The results showed the efficiency of MSC migration increased gradually with the increasing of hSDF-1 density. And after MSCs treatment with 50 nmol/L wortmannin, 10 micromol/L LY294002, 50 micromol/L PD98059, 10 micromol/L U73122 and 126 micromol/L AMD3100 respectively, the ability of MSC migration decreased. The ability of MSCs migration obviously decreased when MSCs were treated with U73122, AMD3100. It is concluded that the SDF-1/CXCR4-mediated MSC migration may be related to mitogen-activated protein kinase (MAPK), phosphatidylinositol phospholipase C (PI-PLC) and protein kinase (PKC) signal pathways.
Wang
Xin-Jun
XJ
Laboratory of Function Experiment, People Hospital, Hubei Province, China.
Tang
Jun-Ming
JM
Kong
Xia
X
Guo
Ling-Yun
LY
Yang
Jian-Ye
JY
Zheng
Fei
F
Chen
Long
L
Huang
Yong-Zhang
YZ
Wang
Jia-Ning
JN
D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology*; D002465:Cell Movement; D005419:Flavonoids / Q000494:pharmacology; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D011493:Protein Kinase C / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D019718:Receptors, CXCR4 / Q000378:metabolism; D015398:Signal Transduction
C486264:Cxcr4 protein, rat; D005419:Flavonoids; D019718:Receptors, CXCR4; D011493:Protein Kinase C; C093973:2-(2-amino-3-methoxyphenyl)-4H-1-benzopyran-4-one