Genetic polymorphisms in Thai neonates with hyperbilirubinemia.
OBJECTIVE
Polymorphisms of the UGT1A1 gene, SLCO1B1 gene and GST gene have been associated with significant hyperbilirubinemia. We would like to determine whether the variation of UGT1A1 gene, SLCO1B1 gene and GST gene may play a significant role in neonatal hyperbilirubinemia in Thai infants.


METHODS
Ninety-one study subjects (hyperbilirubinemic group) and 86 control subjects were studied.


RESULTS
The cause of neonatal hyperbilirubinemia could not be identified in 64 infants (70.3%), ABO blood group incompatibility in 14.3% and Glucose-6-phosphate dehydrogenase (G-6-PD) deficiency in 8.8%. In the hyperbilirubinemic group, 23 of 91 (25.3%) infants demonstrated variant of UGT1A1 at nucleotides (nt) 211 as compared to 6 of 86 (7%) in the control group (p = 0.001). There were no significant differences between groups in the variants UGT1A1 at nt 686, SLCO1B1 gene at nt 388, 463 and the GST gene. Male infants with G-6-PD deficiency were associated with hyperbilirubinemia (21.2% vs. 4.8% in the control group) with an odds ratio (OR) of 5.37 (p = 0.02). The relationship between G-6-PD and variant in UGT1A1 gene at nt 211 could not be determined.


CONCLUSIONS
Thai infants with variant in the UGT1A1 at nt 211 or with G-6-PD deficiency are at higher risk for developing neonatal hyperbilirubinemia.
Prachukthum
Sariya
S
Department of Pediatrics, Faculty of Medicine, Thammasat University, Pathumthani, Thailand.
Nunnarumit
Pracha
P
Pienvichit
Paneeya
P
Chuansumrit
Ampaiwan
A
Songdej
Daunthida
D
Kajanachumpol
Saowanee
S
Pakakasama
Samart
S
Hongeng
Suradej
S
D000704:Analysis of Variance; D001663:Bilirubin / Q000097:blood; D016022:Case-Control Studies; D016009:Chi-Square Distribution; D004247:DNA / Q000737:chemistry / Q000302:isolation & purification; D004252:DNA Mutational Analysis; D005260:Female; D005955:Glucosephosphate Dehydrogenase Deficiency / Q000150:complications*; D014453:Glucuronosyltransferase / Q000235:genetics*; D005982:Glutathione Transferase / Q000235:genetics*; D006801:Humans; D051556:Hyperbilirubinemia, Neonatal / Q000209:etiology / Q000235:genetics* / Q000628:therapy; D007231:Infant, Newborn; D027381:Liver-Specific Organic Anion Transporter 1; D008297:Male; D027361:Organic Anion Transporters / Q000235:genetics*; D011110:Polymorphism, Genetic; D012044:Regression Analysis; D012307:Risk Factors; D013785:Thailand; D000099148:UGT1A1 Enzyme
D001663:Bilirubin; D004247:DNA; D014453:Glucuronosyltransferase; D005982:Glutathione Transferase; D027381:Liver-Specific Organic Anion Transporter 1; D027361:Organic Anion Transporters; D000099148:UGT1A1 Enzyme; C503999:SLCO1B1 protein, human