Identification of immunogenic antigen candidate for Chlamydophila pneumoniae diagnosis.
Chlamydophila pneumoniae is a Gram-negative intracellular obligate human pathogen and accounts for 5-10% of cases of community-acquired pneumonia. However, isolating and culturing this pathogen is difficult, so there have been several studies searching for new biomarkers for its diagnosis. In this study, we obtained immunogenic proteins of C. pneumoniae KNIH-1 for diagnosis using immunoproteomics. C. pneumoniae infection sera were selected for the highest index value of C. pneumoniae-specific IgG using microimmunofluorescence (MIF). The detected protein spots in common from C. pneumoniae infection sera using proteome analysis were identified as Omp11, type III secretion system ATPase, and PmpG by LC-MS/MS and MS databases. They were selected as candidate antigens. In addition, using in silico prediction we also identified proteins encoded by Omp11, PmpG and IncA as antigens. And then, IncA acts as an effector by a type III secretion system ATPase, as identified by mass spectrometry, and was selected as a candidate antigen. Thus, we predict proteins encoded by Omp11, the PmpG family and by IncA as candidate diagnostic immunogens.
Park
Sung-Ha
SH
Laboratory of Pathogenic proteomics, Division of Bacterial Respiratory, Center for Infectious Diseases, National Institute of Health, Korea Centers for Disease Control and Prevention, Seoul, Korea.
Kwon
Su-Jin
SJ
Lee
Sun-Jin
SJ
Kim
Young-Chang
YC
Hwang
Kwang Yeon
KY
Kang
Yeon-Ho
YH
Lee
Kwang-Jun
KJ
D000942:Antigens, Bacterial / Q000097:blood* / Q000276:immunology; D001425:Bacterial Outer Membrane Proteins / Q000097:blood / Q000276:immunology; D001426:Bacterial Proteins / Q000097:blood / Q000276:immunology; D015415:Biomarkers / Q000097:blood; D015153:Blotting, Western; D002460:Cell Line; D023521:Chlamydophila Infections / Q000175:diagnosis* / Q000382:microbiology; D016993:Chlamydophila pneumoniae / Q000276:immunology / Q000302:isolation & purification*; D002853:Chromatography, Liquid; D003198:Computer Simulation; D015180:Electrophoresis, Gel, Two-Dimensional; D004797:Enzyme-Linked Immunosorbent Assay; D005455:Fluorescent Antibody Technique; D006801:Humans; D013058:Mass Spectrometry; D010750:Phosphoproteins / Q000097:blood / Q000276:immunology; D040901:Proteomics / Q000379:methods; D012680:Sensitivity and Specificity
D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D015415:Biomarkers; C094163:IncA protein, bacteria; D010750:Phosphoproteins