title: Purification, crystallization and preliminary X-ray diffraction analysis of a plant subtilase.
abstract: The subtilase SBT3 from Solanum lycopersicum (tomato) was purified from a tomato cell culture and crystallized using the sitting-drop vapour-diffusion method. A native data set was collected to 2.5 A resolution at 100 K using synchrotron radiation. For experimental phasing, CsCl-derivative and tetrakis(acetoxymercuri)methane (TAMM) derivative crystals were employed for MIRAS phasing. Three caesium sites and one TAMM site were identified, which allowed solution of the structure.
authors.affiliation: Chemical Genomics Centre, Dortmund, Germany.
authors.forename: Rolf
authors.initials: R
authors.lastname: Rose
authors.forename: Franziska
authors.initials: F
authors.lastname: Huttenlocher
authors.forename: Anna
authors.initials: A
authors.lastname: Cedzich
authors.forename: Markus
authors.initials: M
authors.lastname: Kaiser
authors.forename: Andreas
authors.initials: A
authors.lastname: Schaller
authors.forename: Christian
authors.initials: C
authors.lastname: Ottmann
mesh_terms: D003460:Crystallization; D018360:Crystallography, X-Ray; D018551:Solanum lycopersicum / Q000201:enzymology* / Q000235:genetics; D013381:Subtilisins / Q000032:analysis* / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism
chemical_list: D013381:Subtilisins; C119297:subtilase, plant