The effects of disodium cromoglycate on enhanced adherence of Haemophilus influenzae to A549 cells infected with respiratory syncytial virus.
Nontypeable Haemophilus influenzae (NTHi) secondary infection often complicates respiratory syncytial virus (RSV) infections. Previous studies have revealed that RSV infections enhance NTHi adherence to airway epithelial cells. In this study, we investigated the effects of disodium cromoglycate (DSCG) and corticosteroids, which are frequently used for the treatment of wheezing often related to RSV infections, on the adherence of NTHi to RSV-infected A549 cells. DSCG inhibited enhanced adherence of NTHi to RSV-infected A549 cells, whereas dexamethasone (Dex) and fluticasone propionate (Fp) did not. DSCG suppressed the expression of ICAM-1, which is one of the NTHi receptors. Furthermore, DSCG exhibited an inhibitory effect on RSV infections. It is suggested that DSCG exerts an anti-RSV effect, and consequently attenuates the expression of NTHi receptors.
Fukasawa
Chie
C
Department of Pediatrics, Chiba University, Chiba 260-8670, Japan. xchiex@pop02.odn.ne.jp
Ishiwada
Naruhiko
N
Ogita
Junko
J
Hishiki
Haruka
H
Kohno
Yoichi
Y
D000730:Androstadienes / Q000494:pharmacology / Q000627:therapeutic use; D018927:Anti-Asthmatic Agents / Q000494:pharmacology / Q000627:therapeutic use; D001422:Bacterial Adhesion / Q000187:drug effects*; D001993:Bronchodilator Agents / Q000494:pharmacology / Q000627:therapeutic use; D045744:Cell Line, Tumor; D004205:Cromolyn Sodium / Q000494:pharmacology / Q000627:therapeutic use; D003907:Dexamethasone / Q000494:pharmacology / Q000627:therapeutic use; D004847:Epithelial Cells / Q000378:metabolism / Q000821:virology; D000068298:Fluticasone; D005938:Glucocorticoids / Q000494:pharmacology / Q000627:therapeutic use; D006192:Haemophilus Infections / Q000188:drug therapy*; D006193:Haemophilus influenzae / Q000187:drug effects*; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism; D020545:Respiratory Mucosa / Q000166:cytology; D018357:Respiratory Syncytial Virus Infections / Q000188:drug therapy*; D012136:Respiratory Syncytial Viruses / Q000187:drug effects*
D000730:Androstadienes; D018927:Anti-Asthmatic Agents; D001993:Bronchodilator Agents; D005938:Glucocorticoids; D018799:Intercellular Adhesion Molecule-1; D003907:Dexamethasone; D000068298:Fluticasone; D004205:Cromolyn Sodium