title: [Effect of recombinant Sp1 gene on the collagen expression of hypertrophic scar fibroblasts in vitro].
abstract: OBJECTIVE
  To explore the feasibility of transfecting recombinant Sp1 into hypertrophic scar fibroblasts and investigate the proliferation and collagen I, III synthesis in the transfected cells.
  
  
  METHODS
  Recombinant human Sp1 was transfected into hypertrophic scar fibroblasts with the karyocyte expressive vector. The expression of Sp1, collagen I, III mRNA was tested by real time PCR. The change of cell proliferation was observed with CCK8 colorimeter.
  
  
  RESULTS
  About 30% of transfected hypertrophic scar fibroblasts showed green fluorescence positive. The relative expression of Sp1 mRNA in transfected cells, empty-vector cell or untransfected cells group was 5.26 +/- 0.76, 1.08 +/- 0.18, 1.09 +/- 0.15, respectively, showing a significant difference between thansfected and untransfected cells or between the transfected cells and empty-vector group (P <0.01, n = 5). Expression of collagen I, III mRNA was 2.49 +/- 0.40 and 1.88 +/- 0.30 in transfected cells, 0.96 +/- 0.18 and 0.95 +/- 0.18 in empty-vector cell, and 0.97 +/- 0.15 and 0.93 +/- 0.13 in untransfected cells, respectively, showing a significant difference between thansfected and untransfected cells or between the transfected cells and empty-vector group (P < 0.01, n = 5).
  
  
  CONCLUSIONS
  The hypertrophic scar fibroblasts could be as the target cells of Sp1 gene transfection. Sp1 gene may play an important role in abnormal collagen metabolism in hypertrophic scar.
authors.affiliation: Shanghai Jiaotong University 6th People's Hospital, Shanghai, China.
authors.forename: Song-lin
authors.initials: SL
authors.lastname: Yang
authors.forename: Wei-dong
authors.initials: WD
authors.lastname: Wan
authors.forename: Jiang-hong
authors.initials: JH
authors.lastname: Zheng
authors.forename: Shi-xin
authors.initials: SX
authors.lastname: Zhang
authors.forename: Yun
authors.initials: Y
authors.lastname: Gao
authors.forename: Chen-liang
authors.initials: CL
authors.lastname: Deng
mesh_terms: D049109:Cell Proliferation; D002478:Cells, Cultured; D017439:Cicatrix, Hypertrophic / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D003094:Collagen / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics; D005347:Fibroblasts / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D012333:RNA, Messenger / Q000235:genetics; D011994:Recombinant Proteins / Q000235:genetics; D012867:Skin / Q000378:metabolism; D016329:Sp1 Transcription Factor / Q000235:genetics*; D014162:Transfection
chemical_list: D012333:RNA, Messenger; D011994:Recombinant Proteins; D016329:Sp1 Transcription Factor; D003094:Collagen