[Phenanthrene and anthracene degradation by microorganisms of the genus Rhodococcus].
The cells of Rhodococcus opacus 412 and R. rhodnii 135 were adapted to phenanthrene and anthracene on a solid mineral medium. Preliminary adaptation of the strains accelerated the metabolism of polyaromatic hydrocarbons and provided for the ability of microorganisms to grow on pheanthrene as a sole carbon and energy source in a liquid mineral medium. It was shown that phenanthrene was mineralized by the strains through 7,8-benzocoumarin, 1-hydroxy-2-naphthoaldehyde, 1-hydroxy-2-naphthoic acid, salicylaldehyde, salicylate and catechol to the intermediates of tricarbonic acid cycle and partially transformed with the accumulation of the products of subsequent monooxygenation (3-hydroxyphenanthrene and phenanthrene dihydroxylated not in ortho-position). As a result of the adaptation of the strains to anthracene on a solid mineral medium, the obtained variant of strain R. opacus 412 was able to transform anthracene in a liquid mineral medium to anthraquinone and 6,7-benzocoumarin.
Leneva
N A
NA
Kolomytseva
M P
MP
Baksunov
B P
BP
Golovleva
L A
LA
D000222:Adaptation, Physiological; D000873:Anthracenes / Q000378:metabolism* / Q000494:pharmacology; D001711:Biotransformation / Q000502:physiology; D010616:Phenanthrenes / Q000378:metabolism* / Q000494:pharmacology; D012240:Rhodococcus / Q000254:growth & development / Q000378:metabolism*; D018508:Water Purification / Q000379:methods
D000873:Anthracenes; D010616:Phenanthrenes; C031181:phenanthrene; C034020:anthracene