[Inhibition effect of gambogic acid on MUTZ-1 cell line and its possible mechanism].
This study was aimed to investigate the effects of gambogic acid on the cells of high-risk patients with myelodysplastic syndrome (MDS) in vitro and its mechanism. The inhibition effect of gambogic acid on growth of MUTZ-1 cell line of MDS-RAEB was detected by MTT method. Apoptosis and cell cycle were detected by morphological observation and flow cytometry respectively. The expressions of bax/bcl-2 gene at mRNA level were detected by RT-PCR. The results indicated that the Gambogic acid inhibited the growth of MUTZ-1 cells, the inhibitory rate of gambogic acid with the range of 0.2 - 0.8 microg/ml was enhanced along with increasing of drug concentration. Flow cytometric assay showed that the apoptotic rate of MUTZ-1 cells treated by gambogic acid also was enhanced along with increasing of drug concentration, the apoptotic rates resulting from gambogic acid (0, 0.4, 0.6, 0.8 microg/ml) were (5 +/- 0.5)%, (13 +/- 0.5)%, (37 +/- 0.7)% and (56 +/- 0.6)% respectively. The characteristic changes of apoptosis emerged in MUTZ-1 cells after being exposed to gambogic acid. Gambogic acid could significantly down-regulate the expressions of bcl-2 gene in a dose dependent manner, however, it had no effects on bax gene. It is concluded that within the range of concentration from 0.4 to 0. 8 microg/ml, gambogic acid can inhibit the growth of MUTZ-1 cells by inducing their apoptosis and down-regulating the expression of bcl-2 gene, which may be one of the main mechanisms underlying its antitumor effects.
Hong
Tie-Yan
TY
Department of Hematology, Zhongda Hospital, Southeast University, Jiangsu Province, China.
Chen
Bao-An
BA
Gao
Chong
C
Xia
Guo-Hua
GH
Ding
Jia-Hua
JH
Shao
Ze-Ye
ZY
Song
Yun-Yu
YY
Wang
Ju
J
Cheng
Jian
J
Song
Hui-Hui
HH
Xue
Meng
M
Bao
Wen
W
Zhao
Gang
G
Xao
Wei
W
Wang
Zhen-Zhong
ZZ
D017209:Apoptosis / Q000187:drug effects*; D002460:Cell Line; D005434:Flow Cytometry; D006801:Humans; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D044004:Xanthones / Q000494:pharmacology*; D051028:bcl-2-Associated X Protein / Q000235:genetics*
C494826:BAX protein, human; D019253:Proto-Oncogene Proteins c-bcl-2; D012333:RNA, Messenger; D044004:Xanthones; D051028:bcl-2-Associated X Protein; C052659:gambogic acid