title: Evaluating the potential for enzymatic acrylamide mitigation in a range of food products using an asparaginase from Aspergillus oryzae.
abstract: Asparaginase, an enzyme that hydrolyzes asparagine to aspartic acid, presents a potentially very effective means for reducing acrylamide formation in foods via removal of the precursor, asparagine, from the primary ingredients. An extracellular asparaginase amenable to industrial production was cloned and expressed in Aspergillus oryzae . This asparaginase was tested in a range of food products, including semisweet biscuits, ginger biscuits, crisp bread, French fries, and sliced potato chips. In dough-based applications, addition of asparaginase resulted in reduction of acrylamide content in the final products of 34-92%. Enzyme dose, dough resting time, and water content were identified as critical parameters. Treating French fries and sliced potato chips was more challenging as the solid nature of these whole-cut products limits enzyme-substrate contact. However, by treating potato pieces with asparaginase after blanching, the acrylamide levels in French fries could be lowered by 60-85% and that in potato chips by up to 60%.
authors.affiliation: Novozymes A/S, Krogshoejvej 36, DK-2880 Bagsvaerd, Denmark. hvhe@novozymes.com
authors.forename: Hanne V
authors.initials: HV
authors.lastname: Hendriksen
authors.forename: Beate A
authors.initials: BA
authors.lastname: Kornbrust
authors.forename: Peter R
authors.initials: PR
authors.lastname: Østergaard
authors.forename: Mary A
authors.initials: MA
authors.lastname: Stringer
mesh_terms: D020106:Acrylamide / Q000032:analysis / Q000037:antagonists & inhibitors*; D001215:Asparaginase / Q000008:administration & dosage* / Q000378:metabolism; D001236:Aspergillus oryzae / Q000201:enzymology*; D001939:Bread / Q000032:analysis; D005504:Food Analysis; D005506:Food Contamination / Q000517:prevention & control; D005511:Food Handling / Q000379:methods*; D035281:Plant Tubers / Q000737:chemistry; D011994:Recombinant Proteins / Q000008:administration & dosage; D011198:Solanum tuberosum / Q000737:chemistry
chemical_list: D011994:Recombinant Proteins; D020106:Acrylamide; D001215:Asparaginase