Expression of the sarA family of genes in different strains of Staphylococcus aureus.
Expression of genes involved in the pathogenesis of Staphylococcus aureus is controlled by global regulatory loci, including two-component regulatory systems and transcriptional regulators. The staphylococcal-specific SarA family of transcription regulators control large numbers of target genes involved in virulence, autolysis, biofilm formation, stress responses and metabolic processes, and are recognized as potential therapeutic targets. Expression of some of these important regulators has been examined, mostly in laboratory strains, while the pattern of expression of these genes in other strains, especially clinical isolates, is largely unknown. In this report, a comparative analysis of 10 sarA-family genes was conducted in six different S. aureus strains, including two laboratory (RN6390, SH1000) and four clinical (MW2, Newman, COL and UAMS-1) strains, by Northern and Western blot analyses. Transcription of most of the sarA-family genes showed a strong growth phase-dependence in all strains tested. Among these genes, no difference was observed in expression of the sarA, sarV, sarT and sarU genes, while a major difference was observed in expression of the sarX gene only in strain RN6390. Expression of mgrA, rot, sarZ, sarR and sarS was observed in all strains, but the level of expression varied from strain to strain.
Ballal
Anand
A
Center for Infectious Disease Research and Vaccinology, South Dakota State University, Brookings, SD 57007, USA.|Division of Basic Biomedical Sciences, University of South Dakota, Vermillion, SD 57069, USA.
Manna
Adhar C
AC
Center for Infectious Disease Research and Vaccinology, South Dakota State University, Brookings, SD 57007, USA.|Division of Basic Biomedical Sciences, University of South Dakota, Vermillion, SD 57069, USA.
D001426:Bacterial Proteins / Q000096:biosynthesis* / Q000235:genetics; D015153:Blotting, Western; D004268:DNA-Binding Proteins / Q000096:biosynthesis / Q000235:genetics; D020869:Gene Expression Profiling; D015964:Gene Expression Regulation, Bacterial; D006801:Humans; D005810:Multigene Family; D012329:RNA, Bacterial / Q000032:analysis / Q000235:genetics; D012097:Repressor Proteins / Q000096:biosynthesis / Q000235:genetics; D013203:Staphylococcal Infections / Q000382:microbiology*; D013211:Staphylococcus aureus / Q000235:genetics / Q000378:metabolism; D015534:Trans-Activators / Q000096:biosynthesis* / Q000235:genetics; D014157:Transcription Factors / Q000096:biosynthesis / Q000235:genetics; D014158:Transcription, Genetic
D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; D012329:RNA, Bacterial; D012097:Repressor Proteins; C088021:SarA protein, bacterial; C433204:SarH3 protein, Staphylococcus aureus; C476149:SarS protein, Staphylococcus aureus; C470922:SarU protein, Staphylococcus aureus; C489658:Sarv protein, Staphylococcus aureus; D015534:Trans-Activators; D014157:Transcription Factors; C408643:rot protein, Staphylococcus aureus