Caffeic and chlorogenic acids in Ilex paraguariensis extracts are the main inhibitors of AGE generation by methylglyoxal in model proteins.
The present study concentrates on the evaluation of the anti-glycation effect of some bioactive substances present in yerba maté (Ilex paraguariensis): 5-caffeoylquinic acid, caffeic acid and a sapogenin (oleanolic acid). Bovine serum albumin and histones were incubated in the presence of methylglyoxal with or without the addition of 5-caffeoylquinic acid, caffeic acid and oleanolic acid. After the incubation period, advanced glycation end product (AGE) fluorescence spectra were performed and protein structural changes were evaluated by Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis. Chlorogenic acid, caffeic acid are the main substances responsible for the anti-glycation effect of maté tea.
Gugliucci
A
A
Glycation, Oxidation and Disease Laboratory, Division of Basic Medical Sciences, Touro University-California, Mare Island Building H-83, 1310 Johnson Lane, Vallejo, CA 94592, USA. agugliuc@touro.edu
Bastos
Deborah H Markowicz
DH
Schulze
John
J
Souza
Marina F Ferreira
MF
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D002109:Caffeic Acids / Q000494:pharmacology*; D002417:Cattle; D002726:Chlorogenic Acid / Q000494:pharmacology* / Q000031:analogs & derivatives; D004591:Electrophoresis, Polyacrylamide Gel; D005453:Fluorescence; D017127:Glycation End Products, Advanced / Q000378:metabolism*; D006657:Histones / Q000378:metabolism; D030019:Ilex paraguariensis / Q000737:chemistry*; D009828:Oleanolic Acid / Q000494:pharmacology*; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D011765:Pyruvaldehyde / Q000378:metabolism; D011801:Quinic Acid / Q000031:analogs & derivatives* / Q000494:pharmacology; D012709:Serum Albumin / Q000378:metabolism
D000975:Antioxidants; D002109:Caffeic Acids; D002726:Chlorogenic Acid; D017127:Glycation End Products, Advanced; D006657:Histones; D009828:Oleanolic Acid; D010936:Plant Extracts; D011765:Pyruvaldehyde; D011801:Quinic Acid; D012709:Serum Albumin; C473200:5-caffeoylquinic acid; C040048:caffeic acid