title: Prevalence and significance of human parvovirus variants in skin from primary cutaneous T cell lymphomas, inflammatory dermatoses and healthy subjects.
abstract: Primary cutaneous T cell lymphomas (CTCL) represent a heterogeneous group of T lymphomas. Virus involvement in CTCL pathogenesis has been extensively investigated, but no data are available as to a causative role of parvovirus B19. The prevalence of parvovirus variants (B19, LaL1/K71, V9) was investigated by using two nested PCRs and a genotype-2 semiquantitative PCR (Q-PCR). Parvovirus DNA was detected in similar percentage in healthy skin controls (40%; n = 42), inflammatory dermatoses (ID) (41%; n = 80) and CTCL (34%; n = 76). Among variants, genotype-2 was more prevalent in ID (26%) and CTCL (22%) than in normal skin (14%; p < 0.05). genotype-3 was never found in normal skin and was rare in ID. The only four pathological skin samples with a quantifiable genome copies/mug DNA values in Q-PCR were ID. In conclusion, despite the skin represent a reservoir for genotype-1, parvovirus infection is not involved in the etiopathogenesis of CTCL.
authors.affiliation: Virology Unit, Department of Public Health and Microbiology, Turin University, Turin, Italy.
authors.forename: Francesca
authors.initials: F
authors.lastname: Sidoti
authors.forename: Maria Teresa
authors.initials: MT
authors.lastname: Fierro
authors.forename: Cristina
authors.initials: C
authors.lastname: Costa
authors.forename: Renata
authors.initials: R
authors.lastname: Ponti
authors.forename: Massimiliano
authors.initials: M
authors.lastname: Bergallo
authors.forename: Alessandra
authors.initials: A
authors.lastname: Comessatti
authors.forename: Mara
authors.initials: M
authors.lastname: Fumagalli
authors.forename: Mauro
authors.initials: M
authors.lastname: Novelli
authors.forename: Chiara
authors.initials: C
authors.lastname: Merlino
authors.forename: Rossana
authors.initials: R
authors.lastname: Cavallo
authors.forename: Maria Grazia
authors.initials: MG
authors.lastname: Bernengo
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D004279:DNA, Viral / Q000032:analysis; D003872:Dermatitis / Q000821:virology*; D005260:Female; D006801:Humans; D016410:Lymphoma, T-Cell, Cutaneous / Q000821:virology*; D008297:Male; D008875:Middle Aged; D010322:Parvoviridae Infections / Q000150:complications* / Q000175:diagnosis; D016732:Parvovirus B19, Human / Q000235:genetics / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction; D012867:Skin / Q000821:virology*; D012878:Skin Neoplasms / Q000821:virology*
chemical_list: D004279:DNA, Viral