title: Use of high density antibody arrays to validate and discover cancer serum biomarkers.
abstract: Perhaps the greatest barrier to translation of serum biomarker discoveries is the inability to evaluate putative biomarkers in high throughput validation studies. Here we report on the development, production, and implementation of a high-density antibody microarray used to evaluate large numbers of candidate ovarian cancer serum biomarkers. The platform was shown to be useful for evaluation of individual antibodies for comparative analysis, such as with disease classification, and biomarker validation and discovery. We demonstrate its performance by showing that known tumor markers behave as expected. We also identify several promising biomarkers from a candidate list and generate hypotheses to support new discovery studies.
authors.affiliation: Molecular Diagnostics Program, Public Health Sciences, Fred Hutchinson Cancer Research Center, Seattle, WA 98109-1024, USA.
authors.forename: Christian M
authors.initials: CM
authors.lastname: Loch
authors.forename: Arturo B
authors.initials: AB
authors.lastname: Ramirez
authors.forename: Yan
authors.initials: Y
authors.lastname: Liu
authors.forename: Cassandra L
authors.initials: CL
authors.lastname: Sather
authors.forename: Jeffrey J
authors.initials: JJ
authors.lastname: Delrow
authors.forename: Nathalie
authors.initials: N
authors.lastname: Scholler
authors.forename: Barbara M
authors.initials: BM
authors.lastname: Garvik
authors.forename: Nicole D
authors.initials: ND
authors.lastname: Urban
authors.forename: Martin W
authors.initials: MW
authors.lastname: McIntosh
authors.forename: Paul D
authors.initials: PD
authors.lastname: Lampe
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D000906:Antibodies / Q000235:genetics*; D014408:Biomarkers, Tumor / Q000097:blood*; D016022:Case-Control Studies; D005260:Female; D006801:Humans; D008875:Middle Aged; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D010051:Ovarian Neoplasms / Q000097:blood* / Q000235:genetics* / Q000473:pathology; D015203:Reproducibility of Results
chemical_list: D000906:Antibodies; D014408:Biomarkers, Tumor