title: Assay reproducibility and within-person variation of Müllerian inhibiting substance.
abstract: OBJECTIVE
  To assess reproducibility of a commercial müllerian inhibiting substance (MIS) assay and evaluate within-person variation in serum MIS levels.
  
  
  METHODS
  Assay reproducibility was evaluated by measuring MIS in multiple serum aliquots from the same blood collection. Within-person variation was assessed by measuring MIS in serum collected twice from the same individuals.
  
  
  METHODS
  Cancer Prevention Biomarker and Genotyping Facility, fox Chase Cancer Center, Philadelphia, Pennsylvania.
  
  
  METHODS
  Assay reproducibility was evaluated using serum from five volunteers with regular menstrual cycles. Within-person variation was evaluated in serum from 20 premenopausal women who donated blood twice at least 1 year apart.
  
  
  METHODS
  For both studies, samples were randomly ordered in batches and laboratory personnel were blinded to which aliquots were from the same subject.
  
  
  METHODS
  The MIS was measured by ELISA.
  
  
  RESULTS
  Within- and between-batch coefficients of variation (CVs) of the assay were 7.9% and 12.3%, respectively. After deleting one subject with extreme values, these CVs decreased to 7.6% and 7.7%, respectively. Within- and between-subject variance in MIS measurements were 2.19 and 0.31, respectively, and the intraclass correlation coefficient was 0.88 (95% confidence interval .77-.98).
  
  
  CONCLUSIONS
  The MIS serum concentration is relatively stable over 1 year in premenopausal women and can be measured with good reproducibility using a commercial kit.
authors.affiliation: Fox Chase Cancer Center, Philadelphia, Pennsylvania 19012, USA. joanne.dorgan@fccc.edu
authors.forename: Joanne F
authors.initials: JF
authors.lastname: Dorgan
authors.forename: Cynthia S
authors.initials: CS
authors.lastname: Spittle
authors.forename: Brian L
authors.initials: BL
authors.lastname: Egleston
authors.forename: Christiana M
authors.initials: CM
authors.lastname: Shaw
authors.forename: Lisa L
authors.initials: LL
authors.lastname: Kahle
authors.forename: Louise A
authors.initials: LA
authors.lastname: Brinton
mesh_terms: D000328:Adult; D054304:Anti-Mullerian Hormone / Q000097:blood* / Q000502:physiology; D015415:Biomarkers / Q000097:blood; D019411:Clinical Laboratory Techniques / Q000592:standards*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000592:standards; D005260:Female; D006801:Humans; D010865:Pilot Projects; D015203:Reproducibility of Results
chemical_list: D015415:Biomarkers; D054304:Anti-Mullerian Hormone