A new stromata-producing Neotyphodium species symbiotic with clonal grass Calamagrostis epigeios (L.) Roth. grown in China.
We describe a new stromata-producing Neotyphodium species symbiotic with clonal Calamagrostis epigeios (L.) Roth. Stromata on the grass, 47.5-186 mm long, occurred frequently, but neither perithecium nor mature ascus was observed. Morphology of fungal isolates obtained from symptomatic and asymptomatic tillers were identical to each other and similar to those of epichloë endophytes. In phylogenetic analysis all selected five fungal isolates clustered into a significantly distinct clade based on sequences of beta-tubulin gene (tubB) introns and translation elongation factor 1-alpha gene (tefA) introns with bootstrap values of 99%, supporting erection of a new species. Concerning the production of extremely long stromata on the host plants and absence of sexual spores, we propose the name Neotyphodium stromatolongum Y. Ji, L. Zhan et Z. Wang, sp. nov.
Ji
Yan-ling
YL
Department of Microbiology, College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, China.
Zhan
Li-hui
LH
Kang
Yan
Y
Sun
Xiang-hui
XH
Yu
Han-shou
HS
Wang
Zhi-wei
ZW
D002681:China; D055328:Neotyphodium / Q000145:classification* / Q000166:cytology / Q000302:isolation & purification / Q000502:physiology*; D010802:Phylogeny; D006109:Poaceae / Q000254:growth & development / Q000382:microbiology*; D013045:Species Specificity; D013559:Symbiosis / Q000502:physiology