Characterization of O-trimethylsilyl oximes of trisaccharides by gas chromatography-mass spectrometry.
Gas chromatography (GC) data (linear retention indices and relative areas) and mass spectra (most representative m/z fragments) of 12 reducing trisaccharides as trimethylsilyl oximes (TMSO) and four non-reducing as trimethylsilyl (TMS) ethers have been described for the first time and related to their structural features. Some trends have been observed: earlier elution of non-reducing compounds and fructotrioses; aldotrioses bearing the reducing end with link in position 6 showing the highest retention. Abundance of several fragment ions and their ratios were useful for trisaccharide characterization; some of these features seem to be useful for the characterization of new trisaccharides.
Brokl
Michał
M
Instituto de Química Orgánica General, Juan de la Cierva, Madrid, Spain.
Soria
Ana Cristina
AC
Martínez-Castro
Isabel
I
Sanz
Maria Luz
ML
Ruiz-Matute
Ana Isabel
AI
D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D010091:Oximes / Q000737:chemistry*; D014312:Trisaccharides / Q000737:chemistry*
D010091:Oximes; D014312:Trisaccharides