Dynamic 3D culture promotes spontaneous embryonic stem cell differentiation in vitro.
Spontaneous in vitro differentiation of mouse embryonic stem cells (mESC) is promoted by a dynamic, three-dimensional (3D), tissue-density perfusion technique with continuous medium perfusion and exchange in a novel four-compartment, interwoven capillary bioreactor. We compared ectodermal, endodermal, and mesodermal immunoreactive tissue structures formed by mESC at culture day 10 with mouse fetal tissue development at gestational day E9.5. The results show that the bioreactor cultures more closely resemble mouse fetal tissue development at gestational day E9.5 than control mESC cultured in Petri dishes.
Gerlach
Jörg C
JC
Department of Surgery and Bioengineering, McGowan Institute for Regenerative Medicine, University of Pittsburgh , Pittsburgh, PA, USA. joerg.gerlach@cellnet.org
Hout
Mariah
M
Edsbagge
Josefina
J
Björquist
Petter
P
Lübberstedt
Marc
M
Miki
Toshio
T
Stachelscheid
Harald
H
Schmelzer
Eva
E
Schatten
Gerald
G
Zeilinger
Katrin
K
D000818:Animals; D019149:Bioreactors; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D018920:Coculture Techniques; D003470:Culture Media / Q000494:pharmacology; D053595:Embryonic Stem Cells / Q000166:cytology*; D021621:Imaging, Three-Dimensional / Q000379:methods*; D051379:Mice; D046529:Microscopy, Electron, Transmission / Q000379:methods; D008856:Microscopy, Fluorescence / Q000379:methods; D010100:Oxygen / Q000737:chemistry; D010477:Perfusion; D013997:Time Factors; D023822:Tissue Engineering / Q000379:methods
D003470:Culture Media; D010100:Oxygen