title: Pyrosequencing analysis of 20 nucleotides of internal transcribed spacer 2 discriminates Candida parapsilosis, Candida metapsilosis, and Candida orthopsilosis.
abstract: Two new cryptic sister species, Candida orthopsilosis and Candida metapsilosis, were recently identified by consistent DNA sequence differences among several genes within the genetically heterogeneous Candida parapsilosis complex. Here, we present data demonstrating that Pyrosequencing analysis of 20 nucleotides of internal transcribed spacer region 2 rapidly and robustly distinguishes between these three closely related Candida species.
authors.affiliation: Mycology Reference Laboratory, Health Protection Agency, Bristol, United Kingdom. Andy.Borman@uhBristol.nhs.uk
authors.forename: Andrew M
authors.initials: AM
authors.lastname: Borman
authors.forename: Christopher J
authors.initials: CJ
authors.lastname: Linton
authors.forename: Debra
authors.initials: D
authors.lastname: Oliver
authors.forename: Michael D
authors.initials: MD
authors.lastname: Palmer
authors.forename: Adrien
authors.initials: A
authors.lastname: Szekely
authors.forename: Frank C
authors.initials: FC
authors.lastname: Odds
authors.forename: Elizabeth M
authors.initials: EM
authors.lastname: Johnson
mesh_terms: D002175:Candida / Q000145:classification* / Q000235:genetics*; D021903:DNA, Ribosomal Spacer / Q000737:chemistry* / Q000235:genetics*; D006801:Humans; D012680:Sensitivity and Specificity; D017422:Sequence Analysis, DNA / Q000379:methods*
chemical_list: D021903:DNA, Ribosomal Spacer