New anthracene glycosides from Rhodomyrtus tomentosa stimulate osteoblastic differentiation of MC3T3-E1 cells.
Two new anthracene glycosides (1, 2) were isolated from aerial parts of Rhodomyrtus tomentosa, along with three known compounds (3-5). The structures of two new compounds were established to be 4,8,9,10-tetrahydroxy-2,3,7-trimethoxyanthracene-6-O-beta-D-glucopyranoside (1) and 2,4,7,8,9,10-hexahydroxy-3-methoxyanthracene-6-O-alpha-L-rhamnopyranoside (2) based on spectroscopic and chemical methods. Among them, compound 1, 2, and 5 significantly (P<0.05) increased the alkaline phosphatase activity, collagen synthesis, and mineralization of the nodules of MC3T3-E1 osteoblastic cells compared to those of the control, respectively.
Tung
Nguyen Huu
NH
College of Pharmacy, Chungnam National University, Daejeon, 305-764, Korea.
Ding
Yan
Y
Choi
Eun Mi
EM
Van Kiem
Phan
P
Van Minh
Chau
C
Kim
Young Ho
YH
D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D000873:Anthracenes / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D002113:Calcification, Physiologic / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects*; D002460:Cell Line; D003094:Collagen / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D006027:Glycosides / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D051379:Mice; D008810:Mice, Inbred C57BL; D015394:Molecular Structure; D027822:Myrtaceae / Q000737:chemistry; D010006:Osteoblasts / Q000187:drug effects* / Q000378:metabolism; D010012:Osteogenesis / Q000187:drug effects*; D035261:Plant Components, Aerial
D000873:Anthracenes; D006027:Glycosides; D003094:Collagen; D000469:Alkaline Phosphatase