Genomic characterization of swine caliciviruses representing a new genus of Caliciviridae.
This study reports the molecular characterization of novel caliciviruses, the St-Valérien-like viruses, which were isolated from pig feces in the province of Quebec, Canada between 2005 and 2007. The genomes of St-Valérien-like viruses contain 6409 nucleotides and include two main open reading frames (ORFs). ORF1 encodes the non structural (NS) polyprotein and the major capsid protein (VP1) while ORF2 encodes the putative basic minor capsid protein. Typical conserved amino acid motifs predict a gene order reminiscent of calicivirus genomes. Phylogenetic, pairwise homology, and distance analyses performed on complete genomic sequences and partial amino acid sequences from the NTPase, polymerase, and major capsid protein segregated the St-Valérien-like viruses in a unique cluster sharing a common root with the Tulane virus and the noroviruses. Based on the genomic analyses presented, the St-Valérien-like viruses are members of a new genus of Caliciviridae for which we propose the name Valovirus.
L'Homme
Yvan
Y
St-Hyacinthe Laboratory, Canadian Food Inspection Agency, 3400 Blvd Casavant West, St-Hyacinthe, QC J2S 8E3, Canada. yvan.lhomme@inspection.gc.ca
Sansregret
Raphaël
R
Plante-Fortier
Etienne
E
Lamontagne
Anne-Marie
AM
Ouardani
Mourad
M
Lacroix
Geneviève
G
Simard
Carole
C
D000818:Animals; D002139:Caliciviridae / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D017250:Caliciviridae Infections / Q000662:veterinary* / Q000821:virology; D016000:Cluster Analysis; D016679:Genome, Viral; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010802:Phylogeny; D011792:Quebec; D012367:RNA, Viral / Q000235:genetics; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D013552:Swine; D013553:Swine Diseases / Q000821:virology*; D014764:Viral Proteins / Q000235:genetics
D012367:RNA, Viral; D014764:Viral Proteins