title: Improvement of chloroperoxidase stability by covalent immobilization on chitosan membranes.
abstract: Chloroperoxidase (CPO) from Caldariomyces fumago was optimally covalently immobilized on chitosan membranes pretreated with 0.8 M glutaraldehyde at pH 3.5 to give 3.18 mg CPO g(-1) support. Using monochlorodimedone (MCD) as assay substrate, the immobilized-CPO retained 40% activity at 50 degrees C after 40 min whereas free CPO retained only 0.02%. The residual activity for immobilized-CPO was 99 and 58% compared with 68 and 43% for free CPO in the presence of 1.5 M urea and 300 microM H(2)O(2), respectively, after 20 h.
authors.affiliation: School of Chemistry and Materials Science, Shaanxi Normal University, Chang'an South Road, 199, Xi'an, Shaanxi, 710062, People's Republic of China.
authors.forename: Li-Hua
authors.initials: LH
authors.lastname: Zhang
authors.forename: Chao-Hong
authors.initials: CH
authors.lastname: Bai
authors.forename: Ying-Song
authors.initials: YS
authors.lastname: Wang
authors.forename: Yu-Cheng
authors.initials: YC
authors.lastname: Jiang
authors.forename: Man-Cheng
authors.initials: MC
authors.lastname: Hu
authors.forename: Shu-Ni
authors.initials: SN
authors.lastname: Li
authors.forename: Quan-Guo
authors.initials: QG
authors.lastname: Zhai
mesh_terms: D001203:Ascomycota / Q000201:enzymology*; D048271:Chitosan / Q000378:metabolism*; D002711:Chloride Peroxidase / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D003512:Cyclohexanones / Q000378:metabolism; D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000737:chemistry / Q000378:metabolism*; D006358:Hot Temperature; D008566:Membranes / Q000201:enzymology*; D013997:Time Factors
chemical_list: D003512:Cyclohexanones; D004800:Enzymes, Immobilized; C006991:chlorodimedone; D048271:Chitosan; D002711:Chloride Peroxidase