title: Registration of planar bioluminescence to magnetic resonance and x-ray computed tomography images as a platform for the development of bioluminescence tomography reconstruction algorithms.
abstract: The procedures we propose make possible the mapping of two-dimensional (2-D) bioluminescence image (BLI) data onto a skin surface derived from a three-dimensional (3-D) anatomical modality [magnetic resonance (MR) or computed tomography (CT)] dataset. This mapping allows anatomical information to be incorporated into bioluminescence tomography (BLT) reconstruction procedures and, when applied using sources visible to both optical and anatomical modalities, can be used to evaluate the accuracy of those reconstructions. Our procedures, based on immobilization of the animal and a priori determined fixed projective transforms, should be more robust and accurate than previously described efforts, which rely on a poorly constrained retrospectively determined warping of the 3-D anatomical information. Experiments conducted to measure the accuracy of the proposed registration procedure found it to have a mean error of 0.36+/-0.23 mm. Additional experiments highlight some of the confounds that are often overlooked in the BLT reconstruction process, and for two of these confounds, simple corrections are proposed.
authors.affiliation: Memorial Sloan-Kettering Cancer Center, Department of Neurology, 1275 York Avenue, New York, New York 10065, USA. beattieb@mskcc.org
authors.forename: Bradley J
authors.initials: BJ
authors.lastname: Beattie
authors.forename: Alexander D
authors.initials: AD
authors.lastname: Klose
authors.forename: Carl H
authors.initials: CH
authors.lastname: Le
authors.forename: Valerie A
authors.initials: VA
authors.lastname: Longo
authors.forename: Konstantine
authors.initials: K
authors.lastname: Dobrenkov
authors.forename: Jelena
authors.initials: J
authors.lastname: Vider
authors.forename: Jason A
authors.initials: JA
authors.lastname: Koutcher
authors.forename: Ronald G
authors.initials: RG
authors.lastname: Blasberg
mesh_terms: D000465:Algorithms; D000818:Animals; D007089:Image Enhancement / Q000379:methods; D007090:Image Interpretation, Computer-Assisted / Q000379:methods*; D008163:Luminescent Measurements / Q000295:instrumentation / Q000379:methods* / Q000662:veterinary; D008279:Magnetic Resonance Imaging / Q000295:instrumentation / Q000379:methods* / Q000662:veterinary; D051379:Mice; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013382:Subtraction Technique / Q000295:instrumentation / Q000662:veterinary; D041622:Tomography, Optical / Q000295:instrumentation / Q000379:methods* / Q000662:veterinary; D014057:Tomography, X-Ray Computed / Q000295:instrumentation / Q000379:methods* / Q000662:veterinary; D051598:Whole Body Imaging / Q000295:instrumentation / Q000379:methods* / Q000662:veterinary