[Expression of CD3 complexes by native and UV-irradiated T lymphocytes of human blood after modification by a preparation of leucocytic alpha-interferon].
The influence of leucocytic alpha-interferon in concentrations of 0.01, 0.1, 1, 10, and 100 IU/ml on the expression of CD3 complexes by native and photomodified T lymphocytes of human blood has been studied. It was found that the irradiation of suspensions of T cells by UV-light in doses of 151-906 J/m2 increases, and in a dose of 1359 J/m2, reduces the quantity of CD3 complexes on the surface of their membranes. It was shown that the injection of cytokine in concentrations of 0.01-100 IU/ml into a suspension of irradiated T lymphocytes levels off the immunosuppressive action of maximal doses of UV-radiation (1359 J/m2), inducing the recovery and increase in their antigen-recognizing capacity. The data on the antigen-recognizing capacity of UV-irradiated T lymphocytes of human blood, modified by alpha-interferon, enable one to predict the results of seances of autotransfusion of UV-irradiated blood in the case of combined use of UV-irradiation of blood and cytokinotherapy.
Artiukhov
V G
VG
Putintseva
O V
OV
Koltakov
I A
IA
Vdovina
V A
VA
D017252:CD3 Complex / Q000096:biosynthesis*; D002462:Cell Membrane / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D004307:Dose-Response Relationship, Radiation; D005786:Gene Expression Regulation / Q000187:drug effects* / Q000528:radiation effects*; D006801:Humans; D007155:Immunologic Factors / Q000494:pharmacology*; D016898:Interferon-alpha / Q000494:pharmacology*; D013601:T-Lymphocytes / Q000378:metabolism*; D014466:Ultraviolet Rays
D017252:CD3 Complex; D007155:Immunologic Factors; D016898:Interferon-alpha