Re-differentiation of human articular chondrocytes is not enhanced by a rotary bioreactor system.
ACI is the most widely used cell-based surgical procedure for the repair of articular cartilage defects. The method is based on in vitro chondrocyte cultivation. Two different culture conditions, rotating-wall-vessel bioreactor and static culture, were assessed by their effect on the re-differentiation potential of human articular chondrocytes seeded into a hydrogel scaffold. Gene expression analysis of the tissue-engineered construct revealed no significant difference between the tested systems.
Barlic
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A
Educell d.o.o., Ljubljana, Slovenia. ariana.barlic@educell.si
Kregar-Velikonja
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N
D053674:Aggrecans / Q000378:metabolism; D019149:Bioreactors; D002358:Cartilage, Articular / Q000166:cytology* / Q000378:metabolism; D018929:Cell Culture Techniques; D002454:Cell Differentiation / Q000235:genetics; D002478:Cells, Cultured; D019902:Chondrocytes / Q000166:cytology* / Q000378:metabolism / Q000637:transplantation; D024042:Collagen Type I / Q000378:metabolism; D024043:Collagen Type II / Q000378:metabolism; D015870:Gene Expression; D006801:Humans; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds; D053675:Versicans / Q000378:metabolism
D053674:Aggrecans; D024042:Collagen Type I; D024043:Collagen Type II; D053675:Versicans; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate