title: Design of triazole-tethered glycoclusters exhibiting three different spatial arrangements and comparative study of their affinities towards PA-IL and RCA 120 by using a dna-based glycoarray.
abstract: Sugar-coated chips: Glycoside clusters are valuable tools for carbohydrate-lectin recognition studies. However, the spatial arrangement of the sugar residues is a key issue in the design of high-affinity glycoclusters. Here the affinities of linear and antenna- and calixarene-based galactoside clusters towards two lectins derived from Pseudomonas aeruginosa and Ricinus communis were compared by means of glycoarrays.Interactions between proteins and carbohydrates are involved in a large number of crucial biological events. Many efforts have been devoted to the design and synthesis of unnatural saccharides displaying high affinities towards targeted lectins. Among others, glycoside clusters have proven to be valuable tools for these recognition studies. However, the spatial arrangements of the sugar residues are a key issue in the design of high-affinity glycoclusters. Here, the affinities of linear and antenna- and calixarene-based galactoside clusters against two lectins, derived from Pseudomonas aeruginosa and Ricinus communis, have been compared by means of glycoarrays.
authors.affiliation: Dipartimento di Chimica, Laboratorio di Chimica Organica, Università di Ferrara, Via L. Borsari 46, 44100, Ferrara, Italy.
authors.forename: Lisa
authors.initials: L
authors.lastname: Moni
authors.forename: Gwladys
authors.initials: G
authors.lastname: Pourceau
authors.forename: Jing
authors.initials: J
authors.lastname: Zhang
authors.forename: Albert
authors.initials: A
authors.lastname: Meyer
authors.forename: Sébastien
authors.initials: S
authors.lastname: Vidal
authors.forename: Eliane
authors.initials: E
authors.lastname: Souteyrand
authors.forename: Alessandro
authors.initials: A
authors.lastname: Dondoni
authors.forename: François
authors.initials: F
authors.lastname: Morvan
authors.forename: Yann
authors.initials: Y
authors.lastname: Chevolot
authors.forename: Jean-Jacques
authors.initials: JJ
authors.lastname: Vasseur
authors.forename: Alberto
authors.initials: A
authors.lastname: Marra
mesh_terms: D018829:Adhesins, Bacterial / Q000737:chemistry*; D047250:Calixarenes / Q000737:chemistry; D004247:DNA / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry; D005697:Galactosides / Q000737:chemistry; D006027:Glycosides / Q000138:chemical synthesis / Q000737:chemistry*; D037102:Lectins / Q000737:chemistry*; D046228:Microarray Analysis; D037121:Plant Lectins / Q000737:chemistry*; D014230:Triazoles / Q000737:chemistry*
chemical_list: D018829:Adhesins, Bacterial; D005456:Fluorescent Dyes; D005697:Galactosides; D006027:Glycosides; D037102:Lectins; D037121:Plant Lectins; C017707:Ricinus communis agglutinin-1; D014230:Triazoles; C038692:adhesin, Pseudomonas; D047250:Calixarenes; D004247:DNA