The effects of prometryne on subchronically treated mice evaluated by SCGE assay.
Prometryne is a methylthio-s-triazine herbicide used to control annual broadleaf and grass weeds in many cultivated plants. Significant traces are documented in environment, mainly water, soil and plants used for human and domestic animal nutrition. Data on the toxic effects of prometryne and other methylthio-s-triazine have scorcely been published. The goal of this study was to investigate if prometryne, applied orally, could induce DNA damage in mouse leukocytes, in subchronical in vivo experimental design. Three different doses of prometryne were applied per os repeatedly every 48 hours. After the 7th dose (day 14) and the 14th dose (day 28) blood leucocytes were analyzed by alkaline Single Cell Gel Electrophoresis (Comet) assay. The results of three different comet parameters showed general increase in Olive tail moment, tail length and tail intensity values in treated groups of animals. The increase in measured values was almost proportional to the dose received and the time of exposure. We conclude that prometryne or its metabolic residues have the potential to induce processes that cause genotoxic effects on leukocytes on mice in in vivo repeated exposure.
Dikić
D
D
Department of Animal Physiology, Biology Division, Faculty of Science, University of Zagreb, Rooseveltov trg 6, 10000 Zagreb, Croatia. magistar_djikic1@yahoo.com
Zidovec-Lepej
Snjezana
S
Remenar
Anica
A
Horvat-Knezević
Anica
A
Benković
Vesna
V
Lisicić
D
D
Sajli
Lana
L
Springer
O
O
D000818:Animals; D020552:Comet Assay; D004249:DNA Damage / Q000187:drug effects*; D005260:Female; D006540:Herbicides / Q000008:administration & dosage / Q000633:toxicity*; D007962:Leukocytes / Q000187:drug effects*; D008297:Male; D051379:Mice; D008808:Mice, Inbred CBA; D011400:Prometryne / Q000008:administration & dosage / Q000633:toxicity*; D036401:Toxicity Tests, Chronic
D006540:Herbicides; D011400:Prometryne