Antibacterial discovery in actinomycetes strains with mutations in RNA polymerase or ribosomal protein S12.
We show that selection of drug-resistant bacterial mutants allows the discovery of antibacterial compounds. Mutant strains of a soil-isolated Streptomyces species that does not produce antibacterials synthesize a previously unknown class of antibacterial, which we name piperidamycin. Overall, 6% of non-Streptomyces actinomycetes species and 43% of Streptomyces species that do not produce antibacterials are activated to produce them. The antibacterial-producing mutants all carried mutations in RNA polymerase and/or the ribosomal protein S12.
Hosaka
Takeshi
T
National Food Research Institute, Tsukuba, Ibaraki, Japan.
Ohnishi-Kameyama
Mayumi
M
Muramatsu
Hideyuki
H
Murakami
Kana
K
Tsurumi
Yasuhisa
Y
Kodani
Shinya
S
Yoshida
Mitsuru
M
Fujie
Akihiko
A
Ochi
Kozo
K
D039903:Actinobacteria / Q000235:genetics / Q000378:metabolism; D000900:Anti-Bacterial Agents / Q000032:analysis*; D012321:DNA-Directed RNA Polymerases / Q000235:genetics*; D055808:Drug Discovery; D009154:Mutation; D012269:Ribosomal Proteins / Q000235:genetics*; D013302:Streptomyces / Q000737:chemistry* / Q000235:genetics* / Q000378:metabolism
D000900:Anti-Bacterial Agents; D012269:Ribosomal Proteins; C034212:ribosomal protein S12; D012321:DNA-Directed RNA Polymerases