title: The genome and proteome of a virulent Escherichia coli O157:H7 bacteriophage closely resembling Salmonella phage Felix O1.
abstract: Based upon whole genome and proteome analysis, Escherichia coli O157:H7-specific bacteriophage (phage) wV8 belongs to the new myoviral genus, "the Felix O1-like viruses" along with Salmonella phage Felix O1 and Erwinia amylovora phage phiEa21-4. The genome characteristics of phage wV8 (size 88.49 kb, mol%G+C 38.9, 138 ORFs, 23 tRNAs) are very similar to those of phage Felix O1 (86.16 kb, 39.0 mol%G+C, 131 ORFs and 22 tRNAs) and, indeed most of the proteins have their closest homologs within Felix O1. Approximately one-half of the Escherichia coli O157:H7 mutants resistant to phage wV8 still serotype as O157:H7 indicating that this phage may recognize, like coliphage T4, two different surface receptors: lipopolysaccharide and, perhaps, an outer membrane protein.
authors.affiliation: Public Health Agency of Canada, Laboratory for Foodborne Zoonoses, 110 Stone Road West, Guelph, ON, N1G 3W4, Canada. Andre_Villegas@phac-aspc.gc.ca
authors.forename: Andre
authors.initials: A
authors.lastname: Villegas
authors.forename: Yi-Min
authors.initials: YM
authors.lastname: She
authors.forename: Andrew M
authors.initials: AM
authors.lastname: Kropinski
authors.forename: Erika J
authors.initials: EJ
authors.lastname: Lingohr
authors.forename: Amanda
authors.initials: A
authors.lastname: Mazzocco
authors.forename: Shivani
authors.initials: S
authors.lastname: Ojha
authors.forename: Thomas E
authors.initials: TE
authors.lastname: Waddell
authors.forename: Hans-Wolfgang
authors.initials: HW
authors.lastname: Ackermann
authors.forename: Dianne M
authors.initials: DM
authors.lastname: Moyles
authors.forename: Rafiq
authors.initials: R
authors.lastname: Ahmed
authors.forename: Roger P
authors.initials: RP
authors.lastname: Johnson
mesh_terms: D001425:Bacterial Outer Membrane Proteins / Q000378:metabolism; D003090:Coliphages / Q000145:classification / Q000235:genetics / Q000378:metabolism / Q000472:pathogenicity; D019453:Escherichia coli O157 / Q000821:virology*; D016679:Genome, Viral; D008070:Lipopolysaccharides / Q000378:metabolism; D017900:Myoviridae / Q000145:classification / Q000235:genetics / Q000378:metabolism / Q000472:pathogenicity; D020543:Proteome; D012483:Salmonella Phages / Q000145:classification* / Q000235:genetics; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism; D014774:Virulence
chemical_list: D001425:Bacterial Outer Membrane Proteins; D008070:Lipopolysaccharides; D020543:Proteome; D014764:Viral Proteins