title: Gravimetric determination of acid detergent fiber and lignin in feed: interlaboratory study.
abstract: On the basis of AOAC Official Method 973.18, a collaborative study was performed to determine fractions of acid detergent fiber (ADF) and lignin (ADL) in animal feed. The study used 6 animal feed and forage samples as blind duplicates and involved 22 participants from 7 countries. The samples analyzed covered a range from 3.5 to 70% ADF and from <1 to 20% ADL. Repeatability standard deviations ranged from 0.27 to 0.95% for ADF and from 0.08 to 0.59% for ADL. The reproducibility standard deviations ranged from 0.56 to 2.44% for ADF and from 0.52 to 2.09% for ADL. Analyzed samples comprised clover and grass silages, soy meal, cattle feed, wheat, and sawdust. The studied method has been published as the EN ISO 13906:2008 standard.
authors.affiliation: FOSS Analytical AB, Pål Anders väg 2, SE-26321 Höganäs, Sweden. jmr@foss.dk
authors.forename: Jürgen
authors.initials: J
authors.lastname: Möller
mesh_terms: D000821:Animal Feed / Q000032:analysis*; D000818:Animals; D002417:Cattle; D003902:Detergents / Q000032:analysis* / Q000302:isolation & purification; D004043:Dietary Fiber / Q000032:analysis*; D004355:Drug Stability; D008031:Lignin / Q000302:isolation & purification; D010940:Plant Proteins / Q000302:isolation & purification; D015203:Reproducibility of Results; D012820:Silage; D014908:Triticum / Q000737:chemistry; D014867:Water / Q000032:analysis
chemical_list: D003902:Detergents; D004043:Dietary Fiber; D010940:Plant Proteins; D014867:Water; C036909:lignocellulose; D008031:Lignin