Genome analysis and characterization of a tobacco mosaic virus isolate infecting balsam (Impatiens balsamina).
The complete RNA genomic sequence of a tobacco mosaic virus isolate infecting Impatiens balsamina, designated as TMV-IM, has been determined. The genomic sequence and the predicted gene products of TMV-IM were similar to those of other members of the genus Tobamovirus. The CP ORF of TMV-IM showed sequence identities of 95.0-99.5% with the corresponding ORFs of other TMV strains. Full-length cDNA of TMV-IM was amplified by RT-PCR with a 5'-end primer harboring a T7 promoter sequence and a 3'-end TMV-specific primer. Subsequently, the full-length cDNA was cloned into plasmid vectors. Capped transcripts synthesized from the cDNA clone were highly infectious and caused characteristic symptoms in balsam plants, similar to wild-type TMV-IM and TMV-U1. These results provide definitive evidence for the natural occurrence of TMV in balsam.
Choi
Seung-Kook
SK
Division of Biosciences and Bioinformatics, College of Natural Science, Myong-Ji University, Yongin 449-728, South Korea. viroid73@yahoo.com
Yoon
Ju-Yeon
JY
Chung
Bong-Nam
BN
D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D016679:Genome, Viral; D029069:Impatiens / Q000821:virology*; D010802:Phylogeny; D010935:Plant Diseases / Q000821:virology; D018515:Plant Leaves / Q000821:virology; D012367:RNA, Viral / Q000235:genetics; D017423:Sequence Analysis, RNA; D014027:Tobacco Mosaic Virus / Q000145:classification / Q000235:genetics*
D018076:DNA, Complementary; D012367:RNA, Viral