Biomolecular characterization and protein sequences of the Campanian hadrosaur B. canadensis.
Molecular preservation in non-avian dinosaurs is controversial. We present multiple lines of evidence that endogenous proteinaceous material is preserved in bone fragments and soft tissues from an 80-million-year-old Campanian hadrosaur, Brachylophosaurus canadensis [Museum of the Rockies (MOR) 2598]. Microstructural and immunological data are consistent with preservation of multiple bone matrix and vessel proteins, and phylogenetic analyses of Brachylophosaurus collagen sequenced by mass spectrometry robustly support the bird-dinosaur clade, consistent with an endogenous source for these collagen peptides. These data complement earlier results from Tyrannosaurus rex (MOR 1125) and confirm that molecular preservation in Cretaceous dinosaurs is not a unique event.
Schweitzer
Mary H
MH
North Carolina State University, Raleigh, NC 27695, USA. schweitzer@ncsu.edu
Zheng
Wenxia
W
Organ
Chris L
CL
Avci
Recep
R
Suo
Zhiyong
Z
Freimark
Lisa M
LM
Lebleu
Valerie S
VS
Duncan
Michael B
MB
Vander Heiden
Matthew G
MG
Neveu
John M
JM
Lane
William S
WS
Cottrell
John S
JS
Horner
John R
JR
Cantley
Lewis C
LC
Kalluri
Raghu
R
Asara
John M
JM
D000595:Amino Acid Sequence; D000818:Animals; D001717:Birds / Q000145:classification; D018775:Bone Demineralization Technique; D001857:Bone Matrix / Q000737:chemistry; D003094:Collagen / Q000032:analysis / Q000737:chemistry* / Q000302:isolation & purification; D025061:Dinosaurs / Q000145:classification; D004549:Elastin / Q000032:analysis; D005269:Femur / Q000098:blood supply / Q000737:chemistry* / Q000648:ultrastructure; D005580:Fossils; D006454:Hemoglobins / Q000032:analysis; D007158:Immunologic Techniques; D007797:Laminin / Q000032:analysis; D013058:Mass Spectrometry; D008855:Microscopy, Electron, Scanning; D008969:Molecular Sequence Data; D010011:Osteocytes / Q000648:ultrastructure; D010446:Peptide Fragments / Q000737:chemistry / Q000302:isolation & purification; D010802:Phylogeny; D011506:Proteins / Q000032:analysis / Q000737:chemistry* / Q000302:isolation & purification; D016415:Sequence Alignment
D006454:Hemoglobins; D007797:Laminin; D010446:Peptide Fragments; D011506:Proteins; D003094:Collagen; D004549:Elastin