title: [Study on C21 steroidal glycosides of Cynanchum atratum by LC-MS].
abstract: High performance liquid chromatography coupled with on-line electrospray tandem mass spectrometry (HPLC/ESI-MS/MS) was used to identify C21 steroidal glycosides in the roots of Cynanchum atratum. The structures of C21 steroidal glycosides were deduced from mass fragments features in positive and negative mode. The constituents of C. atratum were separated and detected. 7 compounds were identified by comparing their ESI-MS/MS data with the reference compounds and 2 compounds were inferred solely by the ESI-MS/MS data. The method is sensitive, and provides good separation and rapid qualitative characterization of C21 steroidal glycosides in the roots of C. atratum.
authors.affiliation: Institute of Materia Medica, Shandong Academy of Medical Sciences, Key Laboratory for Mordern Medicine and Technology of Shandong Province, Jinan 250062, China.
authors.forename: Xinchao
authors.initials: X
authors.lastname: Zhao
authors.forename: Hong
authors.initials: H
authors.lastname: Bai
authors.forename: Yongjun
authors.initials: Y
authors.lastname: Liu
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.forename: Yuanshu
authors.initials: Y
authors.lastname: Wang
authors.forename: Aiqin
authors.initials: A
authors.lastname: Liu
authors.forename: Kazuo
authors.initials: K
authors.lastname: Koike
mesh_terms: D002851:Chromatography, High Pressure Liquid; D031105:Cynanchum / Q000737:chemistry*; D006027:Glycosides / Q000032:analysis* / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization; D013256:Steroids / Q000737:chemistry*; D053719:Tandem Mass Spectrometry
chemical_list: D006027:Glycosides; D013256:Steroids