title: Phosphoester hydrolysis by cerium(IV)-thiacalix[4]arene complexes and its application to immunoassay.
abstract: Thiacalix[4]arenetetrasulfonate was treated with Ce(IV) in water at pH 9.5 to give novel phosphoester-hydrolyzing complexes. The dinuclear Ce(IV) complex promoted the hydrolysis of p-nitrophenyl phosphate with a turnover frequency of 6.8 h(-1) at 50 degrees C, showing fourfold higher activity than the mononuclear complex. The dinuclear complex was readily immobilized onto an antibody by simply mixing them in water, hence its phosphatase-like activity was applied to the color-developing reaction in immunoassay. The model assay using an antibody labeled with the dinuclear complex allowed the detection of as little as 10 ng mL(-1) of a tumor marker, Bence-Jones protein, in a 96-well microtiter plate format. Analysis of urine for Bence-Jones protein was performed by the proposed method.
authors.affiliation: Department of Molecular Design and Engineering, Graduate School of Engineering, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8603, Japan. h-matsu@numse.nagoya-u.ac.jp
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Matsumiya
authors.forename: Hiroko
authors.initials: H
authors.lastname: Nakamura
authors.forename: Masataka
authors.initials: M
authors.lastname: Hiraide
mesh_terms: D000818:Animals; D001536:Bence Jones Protein / Q000032:analysis / Q000276:immunology; D002138:Calibration; D047250:Calixarenes / Q000737:chemistry*; D002384:Catalysis; D004952:Esters / Q000032:analysis* / Q000737:chemistry*; D006801:Humans; D006868:Hydrolysis; D007118:Immunoassay / Q000379:methods*; D007074:Immunoglobulin G / Q000276:immunology; D015394:Molecular Structure; D009596:Nitrophenols / Q000032:analysis* / Q000737:chemistry*; D009942:Organometallic Compounds / Q000737:chemistry*; D009943:Organophosphorus Compounds / Q000032:analysis* / Q000737:chemistry*; D011817:Rabbits
chemical_list: D004952:Esters; D007074:Immunoglobulin G; D009596:Nitrophenols; D009942:Organometallic Compounds; D009943:Organophosphorus Compounds; D047250:Calixarenes; C008644:nitrophenylphosphate; D001536:Bence Jones Protein