title: [Breeding of arachidonic acid producing Mortierella sabellina by ultraviolet mutation].
abstract: OBJECTIVE
  We obtained a new mutant with higher growth rate and high capability of producing arachidonic acid after protoplast ultraviolet mutation.
  
  
  METHODS
  Using dry weight of biomass, microbial total lipid and total arachidonic acid production as evaluation index, we determined time of ultraviolet irradiation by single factor experiment and measured the content of the arachidonic acid by gas chromatography.
  
  
  RESULTS
  When the power of viltalight lamp was set at 20W,exposure distance at 30 cm and exposure time at 80 s, lethality of spores of Mortierella isabellina was 76.4%.
  
  
  CONCLUSIONS
  After ultraviolet mutation and repeatedly screening, a mutat of Mortierella isabellina YZ-124 whose arachidonic acid concentration in biomass was 5.76 times of the control strains was obtained.
authors.affiliation: College of Food Science, Heilongjiang August First Land Reclamation University, Daqing 163319, China. spxyycq@126.com
authors.forename: Changqing
authors.initials: C
authors.lastname: Yu
authors.forename: Lina
authors.initials: L
authors.lastname: Li
mesh_terms: D016718:Arachidonic Acid / Q000378:metabolism*; D001947:Breeding; D003470:Culture Media; D006863:Hydrogen-Ion Concentration; D020094:Mortierella / Q000737:chemistry* / Q000235:genetics*; D009154:Mutation; D013696:Temperature
chemical_list: D003470:Culture Media; D016718:Arachidonic Acid