title: Pharmacological inhibition of aurora-A but not aurora-B impairs interphase microtubule dynamics.
abstract: Aurora kinases are key cell cycle regulators and represent attractive new targets in cancer therapy. In this work we investigated the effect of specific inhibition of Aurora-A and Aurora-B on interphase microtubule dynamics using the GSK6000063A and AZD1152 HQPA compounds respectively. We show that Aurora-A inhibition results in microtubule network disorganization and bundling. Using video microscopy and laser-based photo ablation we demonstrate that Aurora-A inhibition decreases microtubule shrinkage, growth rate, frequency rescue and nucleation. These results open new perspectives on the role of Aurora-A in interphase and might be worth considering in a pharmacological perspective.
authors.affiliation: Université de Toulouse, LBCMCP, Toulouse, France.
authors.forename: Corinne
authors.initials: C
authors.lastname: Lorenzo
authors.forename: Qiaoyin
authors.initials: Q
authors.lastname: Liao
authors.forename: Mary Ann
authors.initials: MA
authors.lastname: Hardwicke
authors.forename: Bernard
authors.initials: B
authors.lastname: Ducommun
mesh_terms: D064107:Aurora Kinase B; D064127:Aurora Kinases; D045744:Cell Line, Tumor; D006367:HeLa Cells; D006801:Humans; D007399:Interphase; D008870:Microtubules / Q000187:drug effects* / Q000502:physiology; D010755:Organophosphates / Q000737:chemistry / Q000494:pharmacology*; D047428:Protein Kinase Inhibitors / Q000737:chemistry / Q000494:pharmacology*; D017346:Protein Serine-Threonine Kinases / Q000037:antagonists & inhibitors* / Q000378:metabolism; D011799:Quinazolines / Q000737:chemistry / Q000494:pharmacology*
chemical_list: C520647:2-((3-((4-((5-(2-((3-fluorophenyl)amino)-2-oxoethyl)-1H-pyrazol-3-yl)amino)quinazolin-7-yl)oxy)propyl)(ethyl)amino)ethyl dihydrogen phosphate; D010755:Organophosphates; D047428:Protein Kinase Inhibitors; D011799:Quinazolines; C576921:AURKB protein, human; D064107:Aurora Kinase B; D064127:Aurora Kinases; D017346:Protein Serine-Threonine Kinases