A cDNA microarray analysis identifies 52 genes associated with cis-diamminedichloroplatinum susceptibility in head and neck squamous cell carcinoma cell lines.
We analyzed the cis-diamminedichloroplatinum (CDDP) susceptibility of ten head and neck squamous cell carcinoma (HNSCC) cell lines and found that this susceptibility varied significantly among the cell lines. Apoptotic cell death was predominant after the CDDP treatment, and a significant association was observed between the induction of apoptosis and the CDDP susceptibility. An analysis using a cDNA microarray consisting of 23,040 genes identified 52 genes that showed altered expression patterns between super-sensitive and super-resistant cell lines after the CDDP treatments. Using these 52 genes, we successfully distinguished the super-resistant cell lines from others. Our present results give us valuable clues to better understand the chemosensitivities of such cells to CDDP. This will improve the clinical management of patients with HNSCC.
Ogawa
Takenori
T
Department of Molecular Pathology, Tohoku University School of Medicine, Sendai Miyagi 980-8575, Japan. ogawa@orl.med.tohoku.ac.jp
Furukawa
Toru
T
Shiga
Kiyoto
K
Hashimoto
Sho
S
Ogawa
Kazumi
K
Kobayashi
Toshimitsu
T
Horii
Akira
A
D000970:Antineoplastic Agents / Q000627:therapeutic use*; D017209:Apoptosis / Q000187:drug effects / Q000235:genetics; D015153:Blotting, Western; D002277:Carcinoma / Q000188:drug therapy / Q000235:genetics / Q000473:pathology; D002294:Carcinoma, Squamous Cell; D045744:Cell Line, Tumor; D002945:Cisplatin / Q000627:therapeutic use*; D018076:DNA, Complementary / Q000032:analysis* / Q000235:genetics; D004273:DNA, Neoplasm / Q000032:analysis* / Q000235:genetics; D005434:Flow Cytometry; D052138:Genes, Neoplasm / Q000235:genetics*; D006258:Head and Neck Neoplasms / Q000188:drug therapy / Q000235:genetics / Q000473:pathology; D006801:Humans; D018307:Neoplasms, Squamous Cell / Q000188:drug therapy / Q000235:genetics / Q000473:pathology; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D000077195:Squamous Cell Carcinoma of Head and Neck
D000970:Antineoplastic Agents; D018076:DNA, Complementary; D004273:DNA, Neoplasm; D002945:Cisplatin