title: ADP-ribosylation factor like 7 (ARL7) interacts with alpha-tubulin and modulates intracellular vesicular transport.
abstract: ADP-ribosylation factor (ARF) like 7 (ARL7, also named ARL4C) is a member of ARL family and recent studies showed that it is involved in the AI-dependent cholesterol secretion process. Yet its biological function remains largely unknown. Using a MALDI-TOF/MS analysis, we identified alpha-tubulin interacted with ARL7. The interaction was confirmed by GST pull-down assay and co-immunoprecipitation in renal carcinoma cell 786-O in which we found the endogenous ARL7 is expressed. This is the second ARL member found interacting with tubulin after ARL8. In addition, ARL7Q72L, a GTP-binding form, promoted the transferrin transport from early endosome to recycling endosome significantly. The above data suggested that ARL7 might modulate the intracellular vesicular transport via interaction with microtubules.
authors.affiliation: Department of Pathology, Second Affiliated Hospital of Zhejiang University, School of Medicine, Jiefang Road 88[#], Hangzhou, Zhejiang Province 310009, China.
authors.forename: Shu-mei
authors.initials: SM
authors.lastname: Wei
authors.forename: Chuan-gao
authors.initials: CG
authors.lastname: Xie
authors.forename: Yasuhito
authors.initials: Y
authors.lastname: Abe
authors.forename: Jian-ting
authors.initials: JT
authors.lastname: Cai
mesh_terms: D020727:ADP-Ribosylation Factors / Q000235:genetics / Q000378:metabolism*; D045744:Cell Line, Tumor; D011992:Endosomes; D006801:Humans; D021381:Protein Transport; D012333:RNA, Messenger / Q000096:biosynthesis; D022161:Transport Vesicles / Q000378:metabolism*; D014404:Tubulin / Q000378:metabolism*
chemical_list: D012333:RNA, Messenger; C533522:TUBA1A protein, human; D014404:Tubulin; D020727:ADP-Ribosylation Factors; C541133:ARL4C protein, human