[Induced differentiation of rat adipose-derived stem cells into Schwann-like cells].
OBJECTIVE
To assess the differentiation potential of rat adipose-derived stem cells (ADSCs) into Schwann-like cells in vitro.


METHODS
ADSCs isolated from adult SD rats were cultured in vitro and identified with the cell surface antigens CD44, CD49d and CD106 by immunocytochemistry. The ADSCs of the sixth to eighth passages were inoculated in polylysine-coated culture plate and cultured for 12 days in DMEM/F12 culture medium containing 10% fetal bovine serum, 5 ng/ml platelet-derived growth factor, 10 ng/ml basic fibroblast growth factor, 14 micromol/L Forskolin and 200 ng/ml Heregulin to induce their differentiation in vitro. Immunocytochemistry was performed to identify the expression of the cell surface markers nestin, glial fibrillary acidic protein (GFAP), S-100, and P75.


RESULTS
The isolated and purified ADSCs were positive for CD44 and CD49d expressions but negative for CD106. After 12 days of culture in the conditional culture medium, most of the cells showed positive expressions of GFAP, S-100, and P75, the specific protein markers of Schwann cells.


CONCLUSIONS
Adult rat ADSCs are confirmed to have potentials of neuroglial differentiation and capable of differentiating into Schwann-like cells in vitro.
Tang
Yin-juan
YJ
Department of Embryology, School of Basic Medical Sciences. Southern Medical University, Guangzhou 510515, China.
Zhang
Li-hua
LH
Liu
Jie-ming
JM
Dong
Wei-ren
WR
Guo
Jia-song
JS
Wang
Hai-hong
HH
Dai
Xiang
X
Chen
Ying-hua
YH
Xiao
Ying-qing
YQ
D000273:Adipose Tissue / Q000166:cytology*; D000818:Animals; D002417:Cattle; D002454:Cell Differentiation; D049109:Cell Proliferation; D003584:Cytological Techniques / Q000379:methods*; D005786:Gene Expression Regulation; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D012583:Schwann Cells / Q000166:cytology* / Q000378:metabolism; D013234:Stem Cells / Q000166:cytology*