[Comparison of distribution of Ophiopogon japonicas polysaccharide in hearts of normal rats and myocardial ischemic rats].
OBJECTIVE
To investigate the distribution of Ophiopogon Japonicas polysaccharide (OJP) in hearts of normal rats and myocardial ischemic rats, and obtain the further evidences for determining the mechanism of its anti-myocardial ischemia.


METHODS
Hearts of normal and myocardial ischemic groups were obtained at 5, 10, 30 and 60 min after once intravenous administration of OJP with the dose of 50 mg/kg. The concentration of OJP in samples was subsequently determined by HPGPC with a fluorescence detector.


RESULTS
The distribution of OJP in hearts of myocardial ischemic rats was much more than that in normal groups at all the sampling times. Moreover, the differences were proved to be significant by SPSS software with the exception of the situation in the 30 min sampling time.


CONCLUSIONS
The enhanced permeability and retention effect caused by ischemia can overcome the negative effect of the reduction of blood flow on the distribution of OJP from blood into myocardial ischemic zones and actually help OJP target myocardial ischemic zones where it can exert its pharmacological effect directly.
Lin
Xiao
X
Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China. shutcm@163.com
Lu
Zhiling
Z
Xu
Desheng
D
Feng
Yi
Y
D000818:Animals; D002138:Calibration; D016650:Fluorescein-5-isothiocyanate / Q000378:metabolism; D008297:Male; D017202:Myocardial Ischemia / Q000378:metabolism*; D009206:Myocardium / Q000378:metabolism*; D031441:Ophiopogon / Q000737:chemistry*; D010539:Permeability; D011134:Polysaccharides / Q000378:metabolism*; D051381:Rats; D012680:Sensitivity and Specificity; D012984:Software; D013997:Time Factors
D011134:Polysaccharides; D016650:Fluorescein-5-isothiocyanate