RGD modified PLGA/gelatin microspheres as microcarriers for chondrocyte delivery.
Poly(lactide-co-glycotide) (PLGA)/gelatin composite microspheres were prepared by an emulsion solvent evaporation technique. RGDS peptides were further immobilized under the catalyzation of water soluble carbodiimide (EDAC). Confocal laser scanning microscopy and transmission electron microscopy revealed that the gelatin was entrapped in the PLGA/gelatin microspheres with a manner of separated domains. The contents of the entrapped gelatin and immobilized RGDS peptides were quantified as 0.9 mg/20 mg and approximately 2.1 microg/20 mg microspheres by hydroxyproline analysis and bicinchoninic acid protein assay, respectively. Moreover, difference in morphology of PLGA, PLGA/gelatin and RGDS modified PLGA/gelatin (PLGA/gelatin-RGDS) microspheres was observed by scanning electron microscopy. The PLGA/gelatin and PLGA/gelatin-RGDS microspheres lost their weight rapidly in PBS, but slowly in DMEM/fetal bovine serum. Rabbit auricular chondrocytes were seeded onto the microspheres in vitro to assess their biological performance and applicability as cell carriers. Results show that amongst the PLGA, PLGA/gelatin and PLGA/gelatin-RGDS microspheres, the latter ones have the best performance in terms of chondrocyte attachment, proliferation, viability and sulfated glycosaminoglycans secretion.
Tan
Huaping
H
Key Laboratory of Macromolecular Synthesis and Functionalization, Ministry of Education, Department of Polymer Science and Engineering, Zhejiang University, Hangzhou 310027, China.
Huang
Dejuan
D
Lao
Lihong
L
Gao
Changyou
C
D000818:Animals; D002417:Cattle; D002478:Cells, Cultured; D019902:Chondrocytes / Q000166:cytology* / Q000378:metabolism; D004337:Drug Carriers / Q000737:chemistry*; D005780:Gelatin / Q000737:chemistry*; D006025:Glycosaminoglycans / Q000737:chemistry / Q000378:metabolism; D019344:Lactic Acid / Q000737:chemistry*; D008422:Materials Testing; D008855:Microscopy, Electron, Scanning; D008863:Microspheres; D009842:Oligopeptides / Q000737:chemistry*; D010316:Particle Size; D011100:Polyglycolic Acid / Q000737:chemistry*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011817:Rabbits
D004337:Drug Carriers; D006025:Glycosaminoglycans; D009842:Oligopeptides; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; C047981:arginyl-glycyl-aspartic acid; D005780:Gelatin