title: Characterization of cefoxitin-resistant Escherichia coli isolates from recreational beaches and private drinking water in Canada between 2004 and 2006.
abstract: A total of 142 cefoxitin-resistant Escherichia coli isolates from water sources were collected across Canada. Multidrug resistance was observed in 65/142 (45.8%) isolates. The bla(CMY-2) gene was identified in 110/142 (77.5%) isolates. Sequencing of the chromosomal ampC promoter region showed mutations from the wild type, previously shown to hyperproduce AmpC. CMY-2-producing plasmids predominantly belonged to replicon groups I1-Igamma, A/C, and K/B. The majority of the E. coli isolates belonged to the nonvirulent phylogenetic groups A and B1.
authors.affiliation: National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, Manitoba, Canada.
authors.forename: L F
authors.initials: LF
authors.lastname: Mataseje
authors.forename: N
authors.initials: N
authors.lastname: Neumann
authors.forename: B
authors.initials: B
authors.lastname: Crago
authors.forename: P
authors.initials: P
authors.lastname: Baudry
authors.forename: G G
authors.initials: GG
authors.lastname: Zhanel
authors.forename: M
authors.initials: M
authors.lastname: Louie
authors.forename: M R
authors.initials: MR
authors.lastname: Mulvey
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000235:genetics; D001493:Bathing Beaches; D002170:Canada; D002440:Cefoxitin / Q000494:pharmacology*; D024881:Drug Resistance, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000187:drug effects* / Q000235:genetics*; D009154:Mutation; D014871:Water Microbiology; D001618:beta-Lactamases / Q000235:genetics
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D002440:Cefoxitin; C103022:beta-lactamase CMY-2; C048795:AmpC beta-lactamases; D001618:beta-Lactamases