Enrichment broth improved detection of extended-spectrum-beta-lactamase-producing bacteria in throat and rectal surveillance cultures of samples from patients in intensive care units.
We evaluated the use of a trypticase soy broth (TSB) for improving detection of extended-spectrum-beta-lactamase-producing (ESBL(+)) bacteria. Preenrichment of throat and rectal swabs in TSB prior to inoculation on solid medium doubled the number of ESBL(+) bacteria detected in samples obtained from patients in our intensive care unit.
Murk
Jean-Luc A N
JL
VU University Medical Center, Medical Microbiology and Infection Control, De Boelelaan 1117, 1081 HV Amsterdam, The Netherlands. j.murk@vumc.nl
Heddema
Edou R
ER
Hess
Dorine L J
DL
Bogaards
Johannes A
JA
Vandenbroucke-Grauls
Christina M J E
CM
Debets-Ossenkopp
Yvette J
YJ
D000328:Adult; D001419:Bacteria / Q000201:enzymology* / Q000302:isolation & purification*; D001431:Bacteriological Techniques / Q000379:methods*; D006801:Humans; D007362:Intensive Care Units; D008403:Mass Screening / Q000379:methods*; D010614:Pharynx / Q000382:microbiology*; D012007:Rectum / Q000382:microbiology*; D012680:Sensitivity and Specificity; D001618:beta-Lactamases / Q000096:biosynthesis*
D001618:beta-Lactamases