Structural probing of RNA thermosensors.
Chemical probing of RNA structure has become one of the most popular approaches to map the conformation of RNA molecules of various sizes under well-defined experimental conditions. The method monitors the sensitivity of each nucleotide to various chemicals, which reflects its hydrogen-bonding environment within the RNA molecule. The goal of this chapter is to provide the reader with an experimental guide to mapping the secondary structure of RNA thermosensors in vitro with the most suitable chemical probes.
Chiaruttini
Claude
C
UPR9073 du CNRS associée à l'Université de Paris VII, Institut de Biologie Physico-Chimique, 75005 Paris, France. claude.chiaruttini@ibpc.fr
Allemand
Frédéric
F
Springer
Mathias
M
D015374:Biosensing Techniques / Q000379:methods*; D007553:Isotope Labeling; D012313:RNA / Q000737:chemistry* / Q000378:metabolism; D012194:RNA-Directed DNA Polymerase / Q000378:metabolism; D011868:Radioisotopes; D038621:Regulatory Sequences, Ribonucleic Acid; D017423:Sequence Analysis, RNA; D013696:Temperature
C034477:RNA primers; D011868:Radioisotopes; D038621:Regulatory Sequences, Ribonucleic Acid; D012313:RNA; D012194:RNA-Directed DNA Polymerase