title: Genistein, a soybean isoflavone, reduces the production of pro-inflammatory and adhesion molecules induced by hemolysate in brain microvascular endothelial cells.
abstract: Genistein (4',5,7-trihydroxyisoflavone) is the most abundant isoflavone found in the soybean that exhibits an anti-inflammatory effect. The present study was designed to examine the effects of genistein on expression levels of hemolysate-induced proinflammatory and adhesion molecules in SD rat brain microvascular endothelial cells (BMECs). Genistein treatment attenuated hemolysate-induced nuclear factor-kappa B (NF-kappaB) p65 translocation in BMECs. In addition, genistein suppressed the expression levels of tumor necrosis factor-alpha (TNF-alpha), monocyte chemoattractant protein 1 (MCP-1), and intercellular adhesion molecule-1 (ICAM-1), but not vascular cell adhesion molecule-1 (VCAM-1). The inhibitory rate of 50 pM genistein for TNF-alpha, MCP-1 and ICAM-1 was 65.4%, 60.5% and 54.9% respectively. These inhibitory effects of genistein on proinflammatory and adhesion molecules were not due to decreased BMEC viability as assessed by MTT test. Taken together the present study suggests that genistein suppresses expression levels of hemolysate-induced pro-inflammatory and adhesion molecules in cerebral endothelial cells.
authors.affiliation: Department of Neurosurgery, Jinling Hospital, Clinical School of Nanjing University, Nanjing, China.
authors.forename: Hua
authors.initials: H
authors.lastname: Lu
authors.forename: Ji-Xin
authors.initials: JX
authors.lastname: Shi
authors.forename: Dong-Mei
authors.initials: DM
authors.lastname: Zhang
authors.forename: Hui-Lin
authors.initials: HL
authors.lastname: Chen
authors.forename: Meng
authors.initials: M
authors.lastname: Qi
authors.forename: Hong-Xia
authors.initials: HX
authors.lastname: Yin
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D001921:Brain / Q000033:anatomy & histology; D015815:Cell Adhesion Molecules / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D002457:Cell Extracts / Q000494:pharmacology*; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D016207:Cytokines / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D042783:Endothelial Cells / Q000187:drug effects*; D004730:Endothelium, Vascular / Q000166:cytology*; D005786:Gene Expression Regulation / Q000187:drug effects; D019833:Genistein / Q000494:pharmacology*; D016328:NF-kappa B / Q000235:genetics / Q000378:metabolism; D048789:Phytoestrogens / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors
chemical_list: D015815:Cell Adhesion Molecules; D002457:Cell Extracts; D016207:Cytokines; D016328:NF-kappa B; D048789:Phytoestrogens; D019833:Genistein