[Selective effect of nispex in inhibiting human cancer cell proliferation and inducing cell apoptosis].
OBJECTIVE
To observe the in vitro anticancer effect of Nispex, the total flavonoids extract from Scurrula parasitic L.


METHODS
The cell proliferation inhibitory effects of Nispex on various kinds of tumor cells or non-tumor cells in human and rats were detected with MTT assay and colony forming assay respectively, the cell apoptosis induced by Nispex was detected by AO/EB fluorescence staining, TUNEL assay and AnnexinV-FITC/PI flow cytometry.


RESULTS
Nispex could significantly inhibit human cancer cell proliferation and induce human cancer cell apoptosis, especially to the proliferative cell group, but its inhibition on human non-tumor cell was insignificant, and showed no effect on murine cancer cells in the tested scope.


CONCLUSIONS
Nispex is a nature plant extract which shows good selectivity for killing human cancer cell.
Xiao
Yi-Jun
YJ
Fujian Institute of Hematology, Union Hospital, Fujian Medical University, Fuzhou.
Chen
Yuan-Zhong
YZ
Chen
Bing-Hua
BH
D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000302:isolation & purification / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000302:isolation & purification; D005419:Flavonoids / Q000302:isolation & purification / Q000494:pharmacology*; D006801:Humans; D028183:Loranthaceae / Q000737:chemistry*; D009369:Neoplasms / Q000473:pathology*; D051381:Rats; D013045:Species Specificity; D014407:Tumor Cells, Cultured
D000972:Antineoplastic Agents, Phytogenic; D004365:Drugs, Chinese Herbal; D005419:Flavonoids