title: The M17 leucine aminopeptidase of the malaria parasite Plasmodium falciparum: importance of active site metal ions in the binding of substrates and inhibitors.
abstract: The M17 leucine aminopeptidase of the intraerythrocytic stages of the malaria parasite Plasmodium falciparum (PfLAP) plays a role in releasing amino acids from host hemoglobin that are used for parasite protein synthesis, growth, and development. This enzyme represents a target at which new antimalarials could be designed since metalloaminopeptidase inhibitors prevent the growth of the parasites in vitro and in vivo. A study on the metal ion binding characteristics of recombinant P. falciparum M17 leucine aminopeptidase (rPfLAP) shows that the active site of this exopeptidase contains two metal-binding sites, a readily exchangeable site (site 1) and a tight binding site (site 2). The enzyme retains activity when the metal ion is removed from site 1, while removal of metal ions from both sites results in an inactive apoenzyme that cannot be reactivated by the addition of divalent metal cations. The metal ion at site 1 is readily exchangeable with several divalent metal ions and displays a preference in the order of preference Zn(2+) > Mn(2+) > Co(2+) > Mg(2+). While it is likely that native PfLAP contains a Zn(2+) in site 2, the metal ion located in site 1 may be dependent on the type and concentration of metal ions in the cytosolic compartment of the parasite. Importantly, the type of metal ion present at site 1 influences not only the catalytic efficiency of the enzyme for peptide substrates but also the mode of binding by bestatin, a metal-chelating inhibitor of M17 aminopeptidases with antimalarial activity.
authors.affiliation: Institute for the Biotechnology of Infectious Diseases (IBID), University of Technology Sydney (UTS), Level 6, Building 4, Corner of Thomas and Harris Street, Ultimo, Sydney, NSW 2007, Australia.
authors.forename: Selma
authors.initials: S
authors.lastname: Maric
authors.forename: Sheila M
authors.initials: SM
authors.lastname: Donnelly
authors.forename: Mark W
authors.initials: MW
authors.lastname: Robinson
authors.forename: Tina
authors.initials: T
authors.lastname: Skinner-Adams
authors.forename: Katharine R
authors.initials: KR
authors.lastname: Trenholme
authors.forename: Donald L
authors.initials: DL
authors.lastname: Gardiner
authors.forename: John P
authors.initials: JP
authors.lastname: Dalton
authors.forename: Colin M
authors.initials: CM
authors.lastname: Stack
authors.forename: Jonathan
authors.initials: J
authors.lastname: Lowther
mesh_terms: D000818:Animals; D001665:Binding Sites; D020134:Catalytic Domain; D004791:Enzyme Inhibitors / Q000737:chemistry* / Q000378:metabolism; D007700:Kinetics; D007931:Leucyl Aminopeptidase / Q000737:chemistry* / Q000378:metabolism; D008670:Metals / Q000737:chemistry / Q000378:metabolism; D010963:Plasmodium falciparum / Q000201:enzymology* / Q000378:metabolism; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D013329:Structure-Activity Relationship; D013379:Substrate Specificity
chemical_list: D004791:Enzyme Inhibitors; D008670:Metals; D011994:Recombinant Proteins; D007931:Leucyl Aminopeptidase