Gene expression profiling of resistant and susceptible Bombyx mori strains reveals nucleopolyhedrovirus-associated variations in host gene transcript levels.
We investigated variations in the gene expression of Bombyx mori following infection with a nucleopolyhedrovirus (BmNPV). Two B. mori strains, KN and 306, which are highly resistant and susceptible to BmNPV infection, respectively, were used in this study. The infection profiles of BmNPV in the B. mori KN and 306 larvae revealed that the virus invaded the midguts of both these strains. However, its proliferation was notably inhibited in the midgut of the resistant strain. By using the suppression subtractive hybridization method, two cDNA libraries were constructed in order to compare the BmNPV responsive gene expressions between the two silkworm lines. In total, 62 differentially expressed genes were obtained. Real-time qPCR analysis confirmed that eight genes were significantly up-regulated in the midgut of the KN strain following BmNPV infection. Our results imply that these up-regulated genes may be involved in the B. mori immune response against BmNPV infection.
Bao
Yan-Yuan
YY
Key Laboratory of Molecular Biology of Crop Pathogens and Insects, Ministry of Agriculture, Institute of Insect Sciences, Zhejiang University, Hangzhou, 310029, China.
Tang
Xu-Dong
XD
Lv
Zu-Yao
ZY
Wang
Xiu-Ye
XY
Tian
Cai-Hong
CH
Xu
Yi Peng
YP
Zhang
Chuan-Xi
CX
D000818:Animals; D001483:Base Sequence; D012831:Bombyx / Q000235:genetics* / Q000648:ultrastructure / Q000821:virology*; D018076:DNA, Complementary / Q000235:genetics / Q000302:isolation & purification; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D014644:Genetic Variation; D007814:Larva / Q000648:ultrastructure / Q000821:virology; D046529:Microscopy, Electron, Transmission; D017924:Nucleopolyhedroviruses / Q000235:genetics*; D016133:Polymerase Chain Reaction; D014158:Transcription, Genetic
D018076:DNA, Complementary