Beneficial effects of Vitis coignetiae Pulliat leaves on nonalcoholic steatohepatitis in a rat model.
Vitis coignetiae Pulliat (Yamabudo) is used as a health juice and wine based on the abundant polyphenols and anthocyanins in its fruit. However, it is not known whether the leaves of this plant confer similar benefits. This study investigated the hepatoprotective effects of aqueous extracts from Vitis coignetiae Pulliat leaves (VCPL) in an animal model of nonalcoholic steatohepatitis (NASH). Rats were fed a choline-deficient high-fat diet for four weeks to generate fatty livers. NASH was induced by oxidative stress loading. Ten weeks later, blood and liver samples were collected from anesthetized animals and assessed biochemically, histologically, and histochemically to determine the extent of oxidative stress injury and the overall effects of VCPL. Six-week VCPL extract supplementation reduced serum levels of liver enzymes, decreased CYP2E1 induction, increased plasma antioxidant activities and delayed the progression of liver fibrosis. The findings suggested that VCPL has strong radical-scavenging activity and may be beneficial in preventing NASH progression.
Takayama
Fusako
F
Department of Clinical Pharmaceutical Science, Okayama University, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama 700-8530, Japan. takayamaf@cc.okayama-u.ac.jp
Nakamoto
Kazuo
K
Kawasaki
Hiromu
H
Mankura
Mitsumasa
M
Egashira
Toru
T
Ueki
Keiji
K
Hasegawa
Azusa
A
Okada
Shigeru
S
Mori
Akitane
A
D000818:Animals; D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology / Q000627:therapeutic use; D019392:Cytochrome P-450 CYP2E1 / Q000378:metabolism; D019587:Dietary Supplements; D004195:Disease Models, Animal; D004790:Enzyme Induction / Q000187:drug effects; D005234:Fatty Liver / Q000188:drug therapy* / Q000473:pathology; D008297:Male; D008862:Microsomes, Liver / Q000187:drug effects / Q000201:enzymology; D018384:Oxidative Stress; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology / Q000627:therapeutic use*; D018515:Plant Leaves / Q000737:chemistry*; D051381:Rats; D017208:Rats, Wistar; D027843:Vitis / Q000033:anatomy & histology / Q000737:chemistry
D000975:Antioxidants; D010936:Plant Extracts; D019392:Cytochrome P-450 CYP2E1