Hepatoprotective activity of six polyherbal formulations in CCl4-induced liver toxicity in mice.
To evaluate pretreatment of six polyherbal liquid formulations (PLFs) commercially available in India, on CCl4-induced liver injury, Swiss albino mice were treated for 7 days with distilled water or PLFs (2.6 and 5.2 ml/kg body weight/day, po) followed by single sc injection of 50% (v/v) CCl4 in arachis oil at a dose of 1 ml/kg. The serum biochemical parameters such as alanine transaminases, aspartate transaminases and alkaline phosphatase were estimated. Phenobarbitone-induced sleeping time and liver histopathology were also carried out. CCl4-treated animals showed significant increase in the levels of liver enzymes, phenobarbitone-induced sleeping time and revealed fatty changes and centrizonal necrosis on histological examination of liver indicating hepatic damage. When pretreated with PLFs at a dose of 5.2 ml/kg body weight/day, the CCl4-induced changes were significantly reversed. The pretreatment with PLFs can prevent acute liver damage induced by CCl4 only at a higher dose. Therefore, it is suggested that a dose adjustment of these PLFs may be necessary for their optimal effects in human liver diseases.
Girish
C
C
Department of Pharmacology, Jawaharlal Institute of Postgraduate Medical Education and Research, Pondicherry, India. gcnx@rediffmail.com
Koner
Bidhan Chandra
BC
Jayanthi
S
S
Rao
K Ramachandra
KR
Rajesh
B
B
Pradhan
Suresh Chandra
SC
D000818:Animals; D002251:Carbon Tetrachloride; D056486:Chemical and Drug Induced Liver Injury; D008099:Liver / Q000187:drug effects / Q000201:enzymology / Q000473:pathology*; D008107:Liver Diseases / Q000188:drug therapy* / Q000473:pathology / Q000517:prevention & control*; D051379:Mice; D010634:Phenobarbital / Q000494:pharmacology; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology / Q000627:therapeutic use*; D020011:Protective Agents / Q000494:pharmacology / Q000627:therapeutic use*; D012890:Sleep / Q000187:drug effects; D013997:Time Factors
D010936:Plant Extracts; D020011:Protective Agents; D002251:Carbon Tetrachloride; D010634:Phenobarbital