title: Characterization of human skeletal muscle biopsy samples using shotgun proteomics.
abstract: We characterized the human muscle proteome by studying muscle biopsy specimens through four different workflows, using 1 or 2D peptide separation, SDS gels, or differential solubilization. By performing MS/MS analyses of 178 4-h LC separations derived from 31 patients, we identified more than 2000 proteins, and determined how 370 very abundant proteins behave upon differential solubilization. The resulting semiquantitative database should serve as a resource for muscle biochemistry.
authors.affiliation: Harvard Partners Center for Genetics and Genomics, Cambridge, MA, USA. kenneth.aprker@virgininstruments.com
authors.forename: Kenneth C
authors.initials: KC
authors.lastname: Parker
authors.forename: Ronan J
authors.initials: RJ
authors.lastname: Walsh
authors.forename: Mohammad
authors.initials: M
authors.lastname: Salajegheh
authors.forename: Anthony A
authors.initials: AA
authors.lastname: Amato
authors.forename: Bryan
authors.initials: B
authors.lastname: Krastins
authors.forename: David A
authors.initials: DA
authors.lastname: Sarracino
authors.forename: Steven A
authors.initials: SA
authors.lastname: Greenberg
mesh_terms: D001706:Biopsy; D002853:Chromatography, Liquid / Q000379:methods; D016000:Cluster Analysis; D030562:Databases, Protein; D015180:Electrophoresis, Gel, Two-Dimensional; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D013058:Mass Spectrometry; D018482:Muscle, Skeletal / Q000473:pathology*; D010455:Peptides / Q000737:chemistry; D040081:Protein Array Analysis; D020543:Proteome / Q000032:analysis / Q000378:metabolism; D040901:Proteomics / Q000379:methods*; D012518:Sarcomeres / Q000378:metabolism; D012995:Solubility
chemical_list: D010455:Peptides; D020543:Proteome