title: A novel single-cell DNA fingerprinting method successfully distinguishes sibling human embryos.
abstract: OBJECTIVE
  To validate a novel system for embryonic DNA fingerprinting which can reliably distinguish sibling embryos from each other.
  
  
  METHODS
  Prospective, randomized, and blinded study.
  
  
  METHODS
  Academic center for reproductive medicine.
  
  
  METHODS
  Blastomeres were obtained from discarded and transferred embryos from six patients undergoing IVF treatment.
  
  
  METHODS
  None.
  
  
  METHODS
  Single lymphocytes from sibling cell lines and blastomeres from sibling day 3 human embryos were evaluated for accurate assignment of relationship using whole genome amplification and single-nucleotide polymorphism microarrays.
  
  
  RESULTS
  Assignment of single-cell relationships was accomplished with 100% accuracy. We also observed complete agreement between the molecular karyotype and DNA fingerprint-based identification of embryos implanted in three clinical IVF cases after multiple embryo transfer.
  
  
  CONCLUSIONS
  These data demonstrate the first single-blastomere DNA fingerprinting technology capable of unequivocal discrimination of sibling human embryos.
authors.affiliation: Reproductive Medicine Associates of New Jersey, Morristown, New Jersey 07960, USA. ntreff@rmanj.com
authors.forename: Nathan R
authors.initials: NR
authors.lastname: Treff
authors.forename: Jing
authors.initials: J
authors.lastname: Su
authors.forename: Xin
authors.initials: X
authors.lastname: Tao
authors.forename: Kathleen A
authors.initials: KA
authors.lastname: Miller
authors.forename: Brynn
authors.initials: B
authors.lastname: Levy
authors.forename: Richard T
authors.initials: RT
authors.lastname: Scott
mesh_terms: D001402:B-Lymphocytes / Q000502:physiology; D001757:Blastomeres / Q000166:cytology* / Q000502:physiology*; D015193:Chorionic Villi Sampling; D016172:DNA Fingerprinting / Q000379:methods*; D004624:Embryo Transfer; D005260:Female; D005307:Fertilization in Vitro; D055106:Genome-Wide Association Study; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis; D020641:Polymorphism, Single Nucleotide; D011247:Pregnancy; D011446:Prospective Studies; D035781:Siblings