Mycobacterium riyadhense sp. nov., a non-tuberculous species identified as Mycobacterium tuberculosis complex by a commercial line-probe assay.
A non-chromogenic, slowly growing Mycobacterium strain was isolated from a maxillary sinus lavage from a symptomatic patient in Riyadh, Saudi Arabia. It was initially identified as a member of the Mycobacterium tuberculosis complex by a commercial line-probe assay. Its 16S rRNA, hsp65 and rpoB gene and 16S-23S internal transcribed spacer sequences were unique; phylogenetic analysis based on the 16S rRNA gene sequence groups this organism close to Mycobacterium szulgai and Mycobacterium malmoense. Its unique biochemical properties and mycolic acid profile support separate species status. We propose the name Mycobacterium riyadhense sp. nov. to accommodate this strain. The type strain is NLA000201958(T) (=CIP 109808(T) =DSM 45176(T)).
van Ingen
Jakko
J
National Mycobacteria Reference Laboratory, National Institute for Public Health and the Environment, Bilthoven, The Netherlands. jakko.van.ingen@rivm.nl
Al-Hajoj
Sahal A M
SA
Boeree
Martin
M
Al-Rabiah
Fahad
F
Enaimi
Mimount
M
de Zwaan
Rina
R
Tortoli
Enrico
E
Dekhuijzen
Richard
R
van Soolingen
Dick
D
D000328:Adult; D001426:Bacterial Proteins / Q000235:genetics; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000235:genetics; D003951:Diagnostic Errors; D020459:Genes, rRNA; D006801:Humans; D008297:Male; D008443:Maxillary Sinus / Q000382:microbiology*; D015523:Maxillary Sinusitis / Q000175:diagnosis / Q000382:microbiology; D015336:Molecular Probe Techniques; D008969:Molecular Sequence Data; D009161:Mycobacterium / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology; D009164:Mycobacterium Infections / Q000175:diagnosis / Q000382:microbiology; D009169:Mycobacterium tuberculosis / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D009171:Mycolic Acids / Q000032:analysis; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012338:RNA, Ribosomal, 23S / Q000235:genetics; D011933:Reagent Kits, Diagnostic; D012529:Saudi Arabia; D017422:Sequence Analysis, DNA; D013045:Species Specificity; D007507:Therapeutic Irrigation; D014376:Tuberculosis / Q000175:diagnosis / Q000382:microbiology; D055815:Young Adult
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D021903:DNA, Ribosomal Spacer; D009171:Mycolic Acids; D012336:RNA, Ribosomal, 16S; D012338:RNA, Ribosomal, 23S; D011933:Reagent Kits, Diagnostic