Steroids from the leaves of Chinese Melia azedarach and their cytotoxic effects on human cancer cell lines.
Three new (1-3) and several known (4-6) steroids were isolated from the leaves of Chinese Melia azedarach. The structures of the new compounds were elucidated by means of spectroscopic methods including 2D NMR techniques and mass spectrometry to be (20S)-5,24(28)-ergostadiene-3beta,7alpha,16beta,20-tetrol (1), (20S)-5-ergostene-3beta,7alpha,16beta,20-tetrol (2), and 2alpha,3beta-dihydro-5-pregnen-16-one (3). The cytotoxicities of the isolated compounds against three human cancer cell lines (A549, H460, U251) were evaluated; only compounds 1, 2, and (20S)-5-stigmastene-3beta,7alpha,20-triol (4) were found to show significant cyctotoxic effects with IC(50)s from 12.0 to 30.1 microg/mL.
Wu
Shi-Biao
SB
Department of Natural Products for Chemical Genetic Research, Key Laboratory of Brain Functional Genomics, Ministry of Education, East China Normal University, Shanghai, PR China.
Ji
Yan-Ping
YP
Zhu
Jing-Jing
JJ
Zhao
Yun
Y
Xia
Gang
G
Hu
Ying-He
YH
Hu
Jin-Feng
JF
D000970:Antineoplastic Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology* / Q000633:toxicity; D045744:Cell Line, Tumor; D002681:China; D006801:Humans; D020128:Inhibitory Concentration 50; D009682:Magnetic Resonance Spectroscopy; D031586:Melia azedarach / Q000737:chemistry*; D018515:Plant Leaves / Q000737:chemistry; D013256:Steroids / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology* / Q000633:toxicity
D000970:Antineoplastic Agents; D013256:Steroids