[Role of Radix Platycodi in Tianwang buxinwan decoction analyzed by fingerprints].
OBJECTIVE
To establish the fingerprints of the active fractions from Tian-wang-bu-xin-wan decoction by HPLC, and to explain the role of Radix Platycodi in the decoction prescription.


METHODS
The experimental conditions of the HPLC method were established as follows: Hanbon Lichrospher C18 column (4.6 mm x 250 mm, 5 microm), mobile phase were methanol and 0.012 5 mol L(-1) ammonium acetate/acetic acid buffer, eluted with a linear gradient, flow rate was 1.0 mL min(-1), the photodiode array detector (PDA) and evaporative light scattering detector (ELSD) were connected in series.


RESULTS
In the same conditions and used same method, the extract amount of whole prescription was higher than that of excepting Radix Platycodi. The method provided two kinds of fingerprints with satisfied separation, which were the HPLC-PDA (Max Plot) fingerprint and the HPLC-ELSD fingerprint. And, they had good correlation and complementaritiy.


CONCLUSIONS
The Radix Platycodi can enhance the decoction yield of the prescription; and increase the dissolution and the contents of some ingredients in the decoction.
Liu
Ping
P
Pharmacy of Traditional Chinese Medicine, PLA General Hospital, Beijing 100853, China. liuping0707@yahoo.com.cn
Zou
Peng
P
Li
Wai
W
Sun
Yan
Y
Zhang
Jie
J
Yu
Heshui
H
Zhao
Yang
Y
Ma
Baiping
B
D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D008027:Light; D012542:Scattering, Radiation
D004365:Drugs, Chinese Herbal; C532371:tianwang buxinwan