Comparison of several methods for effective lipid extraction from microalgae.
Various methods, including autoclaving, bead-beating, microwaves, sonication, and a 10% NaCl solution, were tested to identify the most effective cell disruption method. The total lipids from Botryococcus sp., Chlorella vulgaris, and Scenedesmus sp. were extracted using a mixture of chloroform and methanol (1:1). The lipid contents from the three species were 5.4-11.9, 7.9-8.1, 10.0-28.6, 6.1-8.8, and 6.8-10.9 g L(-1) when using autoclaving, bead-beating, microwaves, sonication, and a 10% NaCl solution, respectively. Botryococcus sp. showed the highest oleic acid productivity at 5.7 mg L(-1)d(-1) when the cells were disrupted using the microwave oven method. Thus, among the tested methods, the microwave oven method was identified as the most simple, easy, and effective for lipid extraction from microalgae.
Lee
Jae-Yon
JY
Environmental Biotechnology Research Center, Korea Research Institute of Bioscience and Biotechnology, 111 Gwahangno, Yuseong-gu, Daejeon, Republic of Korea.
Yoo
Chan
C
Jun
So-Young
SY
Ahn
Chi-Yong
CY
Oh
Hee-Mock
HM
D018533:Biomass; D019149:Bioreactors; D002478:Cells, Cultured; D005591:Chemical Fractionation / Q000379:methods*; D002725:Chloroform / Q000737:chemistry; D000460:Chlorophyta / Q000737:chemistry*; D008055:Lipids / Q000302:isolation & purification*; D000432:Methanol / Q000737:chemistry; D012997:Solvents / Q000737:chemistry; D013997:Time Factors
D008055:Lipids; D012997:Solvents; D002725:Chloroform; D000432:Methanol