title: Sulfenic acid--a key intermediate in albumin thiol oxidation.
abstract: The single thiol of human serum albumin (HSA-SH) is the predominant plasma thiol. Both circulating albumin and pharmaceutical preparations are heterogeneous regarding the thiol redox status, as revealed by anion-exchange-hydrophobic interaction chromatography. Sulfenic acid (HSA-SOH) is an intermediate in HSA-SH oxidation processes that was detected through different techniques including mass spectrometry. Recently, quantitative data led to the determination of rate constants. The preferred fate of HSA-SOH is the formation of mixed disulfides. Alternatively, HSA-SOH can be further oxidized to sulfinic and sulfonic acids. Oxidized forms increase under disease conditions, underscoring the importance of HSA-SH as a plasma scavenger of intravascular oxidants. We here provide a critical review of the oxidation of HSA-SH in the context of the intravascular compartment, with emphasis in the methodological approaches of mass spectrometry and chromatography for the analysis of albumin thiol redox states.
authors.affiliation: Laboratorio de Enzimología, Facultad de Ciencias, Universidad de la República, Montevideo, Uruguay.
authors.forename: Lucía
authors.initials: L
authors.lastname: Turell
authors.forename: Horacio
authors.initials: H
authors.lastname: Botti
authors.forename: Sebastián
authors.initials: S
authors.lastname: Carballal
authors.forename: Rafael
authors.initials: R
authors.lastname: Radi
authors.forename: Beatriz
authors.initials: B
authors.lastname: Alvarez
mesh_terms: D002845:Chromatography; D006801:Humans; D013058:Mass Spectrometry; D010084:Oxidation-Reduction; D012709:Serum Albumin / Q000737:chemistry* / Q000378:metabolism; D013434:Sulfenic Acids / Q000737:chemistry*; D013438:Sulfhydryl Compounds / Q000737:chemistry*
chemical_list: D012709:Serum Albumin; D013434:Sulfenic Acids; D013438:Sulfhydryl Compounds