Dry olive leaf extract ameliorates experimental autoimmune encephalomyelitis.
OBJECTIVE
Experimental autoimmune encephalomyelitis (EAE) is an animal model of CNS inflammatory and demyelinating disease multiple sclerosis. Mediterranean diet, rich in olive products is associated with lower incidence of multiple sclerosis in South European population. Therefore, the influence of dry olive leaf extract (DOLE) on EAE course was investigated.


METHODS
Spinal cord homogenate and complete Freund's adjuvant were used for the induction of EAE in Dark Agouti rats. DOLE was applied intragastrically once per day, starting from the day of the immunization. Real time PCR and ELISA were used for the determination of IFN-gamma and IL-17 gene expression and production, respectively.


RESULTS
DOLE reduced various parameters of EAE severity in DA rats, including cumulative disease index, maximal clinical score and disease duration. Also, DOLE decreased cellularity of the draining lymph nodes and production of IFN-gamma and IL-17 by the cells infiltrating spinal cord of EAE rats.


CONCLUSIONS
The results presented in this paper strongly suggest that DOLE-enriched diet has a beneficial effect in EAE in rats. Further studies in humans are required in order to investigate if DOLE could be a useful supplementary dietetic for the patients suffering from multiple sclerosis and other neuroinflammatory disorders.
Miljković
Djordje
D
Institute for Biological Research Sinisa Stanković, Department of Immunology, University of Belgrade, Belgrade, Serbia. georgije_zw@yahoo.com
Dekanski
Dragana
D
Miljković
Zeljka
Z
Momcilović
Miljana
M
Mostarica-Stojkovic
Marija
M
D000818:Animals; D038441:Diet, Mediterranean; D004198:Disease Susceptibility; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000188:drug therapy* / Q000473:pathology; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005620:Freund's Adjuvant / Q000633:toxicity; D007371:Interferon-gamma / Q000235:genetics / Q000378:metabolism; D020381:Interleukin-17 / Q000235:genetics / Q000378:metabolism; D008297:Male; D018696:Neuroprotective Agents / Q000494:pharmacology; D031658:Olea / Q000737:chemistry*; D010936:Plant Extracts / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry; D051381:Rats; D011919:Rats, Inbred Strains; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012720:Severity of Illness Index
D020381:Interleukin-17; D018696:Neuroprotective Agents; D010936:Plant Extracts; D007371:Interferon-gamma; D005620:Freund's Adjuvant