title: Cordycepin inhibits UVB-induced matrix metalloproteinase expression by suppressing the NF-kappaB pathway in human dermal fibroblasts.
abstract: Cordycepin (3-deoxyadenosine) has been shown to exhibit many pharmacological activities, including anti-cancer, anti-inflammatory, and anti-infection activities. However, the anti-skin photoaging effects of cordycepin have not yet been reported. In the present study, we investigated the inhibitory effects of cordycepin on matrix metalloproteinase-1 (MMP-1) and -3 expressions of the human dermal fibroblast cells. Western blot analysis and real-time PCR revealed cordycepin inhibited UVB-induced MMP-1 and -3 expressions in a dose-dependent manner. UVB strongly activated NF-kappaB activity, which was determined by IkappaBalpha degradation, nuclear localization of p50 and p65 subunit, and NF-kappaB binding activity. However, UVB-induced NF-kappaB activation and MMP expression were completely blocked by cordycepin pretreatment. These findings suggest that cordycepin could prevent UVB-induced MMPs expressions through inhibition of NF-kappaB activation. In conclusion, cordycepin might be used as a potential agent for the prevention and treatment of skin photoaging.
authors.affiliation: Department of Biochemistry and Institute for Medical Sciences Korea, Chonbuk National University Medical School, Jeonju 560-182, Korea.
authors.forename: Young Rae
authors.initials: YR
authors.lastname: Lee
authors.forename: Eun Mi
authors.initials: EM
authors.lastname: Noh
authors.forename: Eun Yong
authors.initials: EY
authors.lastname: Jeong
authors.forename: Seok Kweon
authors.initials: SK
authors.lastname: Yun
authors.forename: Young Ju
authors.initials: YJ
authors.lastname: Jeong
authors.forename: Jong Hyeon
authors.initials: JH
authors.lastname: Kim
authors.forename: Kang Beom
authors.initials: KB
authors.lastname: Kwon
authors.forename: Byeong Soo
authors.initials: BS
authors.lastname: Kim
authors.forename: Sung Ho
authors.initials: SH
authors.lastname: Lee
authors.forename: Chang Sik
authors.initials: CS
authors.lastname: Park
authors.forename: Jong Suk
authors.initials: JS
authors.lastname: Kim
mesh_terms: D000375:Aging / Q000502:physiology; D002478:Cells, Cultured; D003839:Deoxyadenosines / Q000494:pharmacology*; D020405:Dermis / Q000166:cytology / Q000187:drug effects / Q000502:physiology / Q000528:radiation effects; D004305:Dose-Response Relationship, Drug; D004790:Enzyme Induction / Q000187:drug effects; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects; D015971:Gene Expression Regulation, Enzymologic; D006801:Humans; D007231:Infant, Newborn; D008297:Male; D020781:Matrix Metalloproteinase 1 / Q000096:biosynthesis / Q000235:genetics / Q000528:radiation effects; D019278:Matrix Metalloproteinase 3 / Q000096:biosynthesis* / Q000235:genetics / Q000528:radiation effects; D061965:Matrix Metalloproteinase Inhibitors; D016328:NF-kappa B / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000378:metabolism; D012867:Skin / Q000503:physiopathology / Q000528:radiation effects; D014466:Ultraviolet Rays
chemical_list: D003839:Deoxyadenosines; D061965:Matrix Metalloproteinase Inhibitors; D016328:NF-kappa B; C511768:MMP3 protein, human; D019278:Matrix Metalloproteinase 3; C522360:MMP1 protein, human; D020781:Matrix Metalloproteinase 1; C058120:cordycepin