Corynebacterium lubricantis sp. nov., isolated from a coolant lubricant.
Three Gram-positive, rod-shaped, oxidase-negative, non-spore-forming, non-motile bacteria (KSS-3Se(T), KSS-4Se and KSS-10Se) were isolated from a coolant lubricant. 16S rRNA gene sequence analyses revealed almost identical sequences, with only a few (<10 positions) differences for these three isolates. Comparisons showed the highest similarities to Corynebacterium pilosum NCTC 11862(T) (97.6 % similarity with strain KSS-3Se(T)). Similarities with other established Corynebacterium species were lower than 97.0 %. Chemotaxonomic data studied for strain KSS-3Se(T) [polar lipids - major compounds phosphatidylglycerol and an unknown glycolipid, moderate amounts of phosphatidylinositol and diphosphatidylglycerol; polyamines (small amounts) - major compounds spermidine and spermine; quinones - significant amounts of menaquinones MK-9(H(2)), MK-8(H(2)) and MK-7(H(2)); and major fatty acids - tuberculostearic acid (10-methyl C(18 : 0)), C(16 : 0) and C(18 : 1)omega9c] were congruent with those reported for Corynebacterium. The strain showed differences in phenotype from C. pilosum. DNA-DNA hybridizations between KSS-3Se(T) and C. pilosum DSM 20521(T) yielded a relatedness of 22.9 % (20.4 % in the reciprocal assay). From these results, it is evident that the organisms represent a novel species, for which the name Corynebacterium lubricantis sp. nov. is proposed (type strain KSS-3Se(T) =DSM 45231(T) =CCUG 56567(T) =CCM 7546(T)).
Kämpfer
Peter
P
Institut für Angewandte Mikrobiologie, Justus-Liebig-Universität Giessen, D-35392 Giessen, Germany. peter.kaempfer@umwelt.uni-giessen.de
Lodders
Nicole
N
Warfolomeow
Isabel
I
Falsen
Enevold
E
Busse
Hans-Jürgen
HJ
D000389:Air Conditioning; D015373:Bacterial Typing Techniques; D003352:Corynebacterium / Q000737:chemistry / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D004269:DNA, Bacterial / Q000032:analysis; D004275:DNA, Ribosomal / Q000032:analysis; D020459:Genes, rRNA; D054327:Lubricants; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010641:Phenotype; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013045:Species Specificity
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D054327:Lubricants; D012336:RNA, Ribosomal, 16S