Expression, crystallization and preliminary crystallographic study of human coronavirus HKU1 nonstructural protein 9.
Human coronavirus HKU1 (HCoV-HKU1) belongs to coronavirus group II and encodes 16 nonstructural proteins (nsps) which mediate genome replication and transcription. Among these nsps, nsp9 has been shown to possess single-stranded DNA/RNA-binding properties. The gene that encodes HCoV-HKU1 nsp9 was cloned and expressed in Escherichia coli and the protein was subjected to crystallization trials. The crystals diffracted to 2.7 A resolution and belonged to space group P2(1)2(1)2, with unit-cell parameters a = 83.5, b = 88.4, c = 31.2 A, alpha = beta = gamma = 90 degrees and two molecules per asymmetric unit.
Wang
Wei
W
Tsinghua University, Beijing, People's Republic of China.
Wei
Lei
L
Yang
Anqi
A
He
Teng
T
Yuen
K Y
KY
Chen
Cheng
C
Rao
Zihe
Z
D003460:Crystallization; D018360:Crystallography, X-Ray; D015870:Gene Expression; D016601:RNA-Binding Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D045473:Severe acute respiratory syndrome-related coronavirus / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D014764:Viral Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism
D016601:RNA-Binding Proteins; D014764:Viral Proteins; C483945:nsp9 protein, SARS virus