Growth-inhibitory effects of a mineralized extract from the red marine algae, Lithothamnion calcareum, on Ca(2+)-sensitive and Ca(2+)-resistant human colon carcinoma cells.
Proliferation and differentiation were assessed in a series of human colon carcinoma cell lines in response to a mineral-rich extract derived from the red marine algae, Lithothamnion calcareum. The extract contains 12% Ca2+, 1% Mg2+, and detectable amounts of 72 trace elements, but essentially no organic material. The red algae extract was as effective as inorganic Ca2+ alone in suppressing growth and inducing differentiation of colon carcinoma cells that are responsive to a physiological level of extracellular Ca2+ (1.4mM). However, with cells that are resistant to Ca2+ alone, the extract was still able to reduce proliferation and stimulate differentiation.
Aslam
Muhammad Nadeem
MN
Department of Pathology, University of Michigan Medical School, 1301 Catherine Street, SPC 5602, Ann Arbor, MI 48109, USA.
Bhagavathula
Narasimharao
N
Paruchuri
Tejaswi
T
Hu
Xin
X
Chakrabarty
Subhas
S
Varani
James
J
D000230:Adenocarcinoma / Q000378:metabolism*; D017209:Apoptosis / Q000187:drug effects; D015153:Blotting, Western; D002454:Cell Differentiation / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D003110:Colonic Neoplasms / Q000378:metabolism*; D045424:Complex Mixtures / Q000494:pharmacology*; D005455:Fluorescent Antibody Technique; D006131:Growth Inhibitors / Q000494:pharmacology; D006801:Humans; D018613:Microscopy, Confocal; D000461:Rhodophyta / Q000737:chemistry*
D045424:Complex Mixtures; D006131:Growth Inhibitors