title: Two-colour two-photon confocal microscopy with isotropic three-dimensional resolution and parallel excitation.
abstract: We demonstrate a novel design of two-colour two-photon fluorescence microscope in which isotropic three-dimensional imaging resolution and high scanning speed can be achieved simultaneously. In our scheme, a three-dimensional optical lattice constructed by multi-beam interference is used for two-colour two-photon fluorescence excitation. Our simulation results show that a resolution of 113.5 nm can be achieved in both transverse and axial directions with two pump pulses at the wavelengths of 400 and 800 nm, respectively; meanwhile, imaging speed can be greatly improved compared with that of traditional two-photon scanning fluorescence microscopes.
authors.affiliation: State Key Laboratory of High Field Laser Physics, Shanghai Institute of Optics and Fine Mechanics, Chinese Academy of Science, P.O. Box 800-211, Shanghai 201800, China.
authors.forename: J
authors.initials: J
authors.lastname: Ni
authors.forename: L
authors.initials: L
authors.lastname: Qiao
authors.forename: C
authors.initials: C
authors.lastname: Wang
authors.forename: F
authors.initials: F
authors.lastname: Zhao
authors.forename: Y
authors.initials: Y
authors.lastname: Cheng
authors.forename: Z
authors.initials: Z
authors.lastname: Xu