title: Transplantation of mesenchymal stem cells cultured on biomatrix support induces repairing of digestive tract defects, in animal model.
abstract: Transplanted mesenchymal stem cells (MSCs) appear to play a significant role in adult tissue repair. The aim of this research was to obtain MSCs enriched, three dimensional (3D) patches for transplant, and to test their ability to induce repair of iatrogenic digestive tract defects in rats. MSCs were obtained from human and rat bone marrow, cultured in vitro, and seeded in a collagen-agarose scaffold, where they showed enhanced viability and proliferation. The phenotype of the cultured cells was representative for MSCs (CD105+, CD90+, and CD34-, CD45-, CD3-, CD14-). The 3D patch was obtained by laying the MSCs enriched collagen-agarose scaffold on a human or swine aortic fragment. After excision of small portions of the rat digestive tract, the 3D patches were sutured at the edge of the defect using micro-surgical techniques. The rats were sacrificed at time-points and the regeneration of the digestive wall was investigated by immunofluorescence, light and electron microscopy. The MSCs enriched 3D patches were biocompatible, biodegradable, and prompted the regeneration of the four layers of the stomach and intestine wall in rats. Human cells were identified in the rat regenerated digestive wall as a hallmark of the transplanted MSCs. For the first time we constructed 3D patches made of cultured bone marrow MSCs, embedded into a collagen-rich biomatrix, on vascular bio-material support, and transplanted them in order to repair iatrogenic digestive tract defects. The result was a complete repair with preservation of the four layered structure of the digestive wall.
authors.affiliation: Fundeni Clinical Institute, Center of General Surgery and Liver Transplantation, Bucharest, Romania. paboet@yahoo.com
authors.forename: Mirela-Patricia
authors.initials: MP
authors.lastname: Sîrbu-Boeţi
authors.forename: Mihaela
authors.initials: M
authors.lastname: Chivu
authors.forename: Liliana Livia
authors.initials: LL
authors.lastname: Pâslaru
authors.forename: C
authors.initials: C
authors.lastname: Efrimescu
authors.forename: V
authors.initials: V
authors.lastname: Herlea
authors.forename: C
authors.initials: C
authors.lastname: Pecheanu
authors.forename: Lucia
authors.initials: L
authors.lastname: Moldovan
authors.forename: Laura
authors.initials: L
authors.lastname: Dragomir
authors.forename: Coralia
authors.initials: C
authors.lastname: Bleotu
authors.forename: Elena
authors.initials: E
authors.lastname: Ciucur
authors.forename: Cristina
authors.initials: C
authors.lastname: Vidulescu
authors.forename: Mihaela
authors.initials: M
authors.lastname: Vasilescu
authors.forename: Anişoara
authors.initials: A
authors.lastname: Boicea
authors.forename: S
authors.initials: S
authors.lastname: Mănoiu
authors.forename: M I
authors.initials: MI
authors.lastname: Ionescu
authors.forename: I
authors.initials: I
authors.lastname: Popescu
mesh_terms: D000818:Animals; D001857:Bone Matrix; D002478:Cells, Cultured; D003094:Collagen; D004195:Disease Models, Animal; D048091:Guided Tissue Regeneration / Q000379:methods; D006801:Humans; D007422:Intestines / Q000293:injuries / Q000601:surgery*; D045164:Mesenchymal Stem Cell Transplantation / Q000379:methods; D008866:Microsurgery / Q000379:methods; D051381:Rats; D013270:Stomach / Q000293:injuries / Q000601:surgery*; D013552:Swine; D023822:Tissue Engineering / Q000379:methods; D016896:Treatment Outcome
chemical_list: D003094:Collagen