title: p75 and TrkA signaling regulates sympathetic neuronal firing patterns via differential modulation of voltage-gated currents.
abstract: Neurotrophins such as nerve growth factor (NGF) and brain-derived neurotrophic factor (BDNF) act through the tropomyosin-related receptor tyrosine kinases (Trk) and the pan-neurotrophin receptor (p75) to regulate complex developmental and functional properties of neurons. While NGF activates both receptor types in sympathetic neurons, differential signaling through TrkA and p75 can result in widely divergent functional outputs for neuronal survival, growth, and synaptic function. Here we show that TrkA and p75 signaling pathways have opposing effects on the firing properties of sympathetic neurons, and define a mechanism whereby the relative level of signaling through these two receptors sets firing patterns via coordinate regulation of a set of ionic currents. We show that signaling through the p75 pathway causes sympathetic neurons to fire in a phasic pattern showing marked accommodation. Signaling through the NGF-specific TrkA, on the other hand, causes cells to fire tonically. Neurons switch rapidly between firing patterns, on the order of minutes to hours. We show that changes in firing patterns are caused by neurotrophin-dependent regulation of at least four voltage-gated currents: the sodium current and the M-type, delayed rectifier, and calcium-dependent potassium currents. Neurotrophin release, and thus receptor activation, varies among somatic tissues and physiological state. Thus, these data suggest that target-derived neurotrophins may be an important determinant of the characteristic electrical properties of sympathetic neurons and therefore regulate the functional output of the sympathetic nervous system.
authors.affiliation: Department of Biology, National Center for Behavioral Genomics, Brandeis University, Waltham, Massachusetts 02454, USA.
authors.forename: Jason A
authors.initials: JA
authors.lastname: Luther
authors.forename: Susan J
authors.initials: SJ
authors.lastname: Birren
mesh_terms: D000320:Adrenergic Fibers / Q000502:physiology*; D000818:Animals; D002478:Cells, Cultured; D015640:Ion Channel Gating / Q000502:physiology; D007473:Ion Channels / Q000502:physiology*; D051379:Mice; D009474:Neurons / Q000502:physiology*; D018408:Patch-Clamp Techniques; D051381:Rats; D020800:Receptor, Nerve Growth Factor / Q000502:physiology*; D020917:Receptor, trkA / Q000502:physiology*; D015398:Signal Transduction / Q000502:physiology*
chemical_list: D007473:Ion Channels; D020800:Receptor, Nerve Growth Factor; D020917:Receptor, trkA