CD40 signaling activated by Epstein-Barr virus promotes cell survival and proliferation in gastric carcinoma-derived human epithelial cells.
CD40 signaling plays a critical role in the survival and proliferation of EBV-infected lymphocytes. Here we show that CD40 is constitutively expressed in the human gastric carcinoma-derived cell lines AGS, MKN28, and MKN74, and expression of CD40L is induced by in vitro infection with EBV. Blocking the interaction between CD40 and CD40L with CD40Ig, a fusion protein of CD40 and IgG, impaired proliferation of EBV-infected AGS cells and enhanced their calcium ionophore-induced apoptosis. These results suggest that CD40 signaling plays a critical role in the survival and proliferation of EBV-infected epithelial cells, as well as in the virus-infected lymphocytes.
Imadome
Ken-Ichi
K
Department of Infectious Diseases, National Research Institute for Child Health and Development, 2-10-1 Okura, Setagaya-ku, Tokyo 157-8535, Japan.
Shimizu
Norio
N
Yajima
Misako
M
Watanabe
Ken
K
Nakamura
Hiroyuki
H
Takeuchi
Hiroaki
H
Fujiwara
Shigeyoshi
S
D019013:CD40 Antigens / Q000378:metabolism*; D023201:CD40 Ligand / Q000096:biosynthesis; D045744:Cell Line, Tumor; D002470:Cell Survival; D004847:Epithelial Cells / Q000502:physiology* / Q000821:virology*; D020869:Gene Expression Profiling; D004854:Herpesvirus 4, Human / Q000502:physiology*; D006801:Humans
D019013:CD40 Antigens; D023201:CD40 Ligand