title: Effect of pro-inflammatory cytokine stimulation on human breast cancer: implications of chemokine receptor expression in cancer metastasis.
abstract: Interactions between tumour cells and microenvironments may affect their growth and metastasis formation. In search for a better understanding of the role of cellular mediators in the progression of cancer, we investigated the effect of pro-inflammatory cytokines IL-1, IL-6, TNF-alpha and IFN-gamma on the regulation of expression of chemokine receptors CXCR4, CXCR2, CX3CR1, CCR9, and CCR5 in the human breast cancer cell line MCF-7. Our results showed that IL-1 increased CXCR4 expression whereas TNF-alpha increased CX3CR1, CCR9 and CCR5. Interestingly, this regulation was not homogeneous, emphasizing the inherent heterogeneity in cancer that may be responsive to specific inflammatory microenvironments.
authors.affiliation: Departamento de Inmunología, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, Ciudad Universitaria, Circuito exterior s/n, C.P. 04510 DF, Mexico.
authors.forename: Julio E
authors.initials: JE
authors.lastname: Valdivia-Silva
authors.forename: Janusz
authors.initials: J
authors.lastname: Franco-Barraza
authors.forename: Ana Luisa Esparza
authors.initials: AL
authors.lastname: Silva
authors.forename: Gisela Du
authors.initials: GD
authors.lastname: Pont
authors.forename: Gloria
authors.initials: G
authors.lastname: Soldevila
authors.forename: Isaura
authors.initials: I
authors.lastname: Meza
authors.forename: Eduardo A
authors.initials: EA
authors.lastname: García-Zepeda
mesh_terms: D001943:Breast Neoplasms / Q000276:immunology* / Q000473:pathology; D045744:Cell Line, Tumor; D002633:Chemotaxis / Q000276:immunology; D016207:Cytokines / Q000276:immunology*; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D009362:Neoplasm Metastasis / Q000276:immunology*; D012333:RNA, Messenger / Q000032:analysis; D019707:Receptors, Chemokine / Q000096:biosynthesis*; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D016207:Cytokines; D012333:RNA, Messenger; D019707:Receptors, Chemokine