title: Expression of thyroglobulin on follicular dendritic cells of thyroid mucosa-associated lymphoid tissue (MALT) lymphoma.
abstract: Reportedly, thyroid mucosa-associated lymphoid tissue (MALT) lymphoma is closely associated with Hashimoto's thyroiditis. However, it remains unknown which antigen is closely associated with thyroid MALT lymphoma. We examined whether B cell response to thyroglobulin (Tg), which is a common thyroid-specific autoantigen, is related etiologically to the pathogenesis of thyroid MALT lymphoma. Expression of human Tg antigens and Cluster of differentiation (CD) 35 was examined immunohistochemically in 15 cases of thyroid MALT lymphoma using paraffin-embedded, formalin-fixed tissue specimens. In all cases of thyroid MALT lymphoma, human Tg was detected immunohistochemically in the follicular epithelial cells and follicular dendritic cells (FDCs). These FDCs were positive by double immunostaining for anti-human Tg rabbit polyclonal antibody (Ab) and for CD35. Results showed that the Tg, a thyroid autoantigen, had immunostained the germinal center of the thyroid MALT lymphoma. The Tg was present in the FDCs, as revealed by the staining pattern of the germinal center;this fact was confirmed by double immunostaining of anti-human Tg mouse monoclonal Ab and anti-CD35 mouse monoclonal Ab. The results of our study suggest that Tg is an autoantigen that is recognized by thyroid MALT lymphoma cells.
authors.affiliation: Department of Pathology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama 700-8558, Japan. mi-kun.munemasa@nifty.ne.jp
authors.forename: Mitsuru
authors.initials: M
authors.lastname: Munemasa
authors.forename: Tadashi
authors.initials: T
authors.lastname: Yoshino
authors.forename: Keita
authors.initials: K
authors.lastname: Kobayashi
authors.forename: Takayoshi
authors.initials: T
authors.lastname: Miyake
authors.forename: Sumie
authors.initials: S
authors.lastname: Takase-Sakugawa
authors.forename: Tomohiko
authors.initials: T
authors.lastname: Mannami
authors.forename: Katsuji
authors.initials: K
authors.lastname: Shinagawa
authors.forename: Mitsune
authors.initials: M
authors.lastname: Tanimoto
authors.forename: Tadaatsu
authors.initials: T
authors.lastname: Akagi
mesh_terms: D000328:Adult; D000368:Aged; D000818:Animals; D001324:Autoantigens / Q000276:immunology; D020566:Dendritic Cells, Follicular / Q000166:cytology / Q000276:immunology*; D005260:Female; D015321:Gene Rearrangement; D006801:Humans; D007143:Immunoglobulin Heavy Chains / Q000235:genetics; D007150:Immunohistochemistry; D018442:Lymphoma, B-Cell, Marginal Zone / Q000235:genetics / Q000276:immunology* / Q000473:pathology; D008297:Male; D051379:Mice; D008875:Middle Aged; D011817:Rabbits; D017463:Receptors, Complement 3b / Q000378:metabolism; D013954:Thyroglobulin / Q000378:metabolism*; D055815:Young Adult
chemical_list: D001324:Autoantigens; C509991:CR1 protein, human; D007143:Immunoglobulin Heavy Chains; D017463:Receptors, Complement 3b; D013954:Thyroglobulin