Biotransformation of L-tyrosine to tyramine by the growing cells of Lactococcus lactis.
The potential of pyridoxal-5-phosphate dependent tyrosine decarboxylase (E.C. 4.1.1.25) of Lactococcus lactis was explored for the biotransformation of L-tyrosine to tyramine. Maximum bioconversion of L-tyrosine to tyramine was achieved in tyramine production medium (pH -5.5) at 30 degrees C after 16 h of incubation with 0.2% L-tyrosine. The yield oftyramine was found to be 11.8 microg/mL by the growing cells of L. lactis at shake flask level. Growth medium and different physico-chemical parameters to maximize the biotransformation of L-tyrosine to tyramine were optimized and yielded 1.9-fold increased synthesis of tyramine.
Thakur
M
M
Department of Biotechnology, Himachal Pradesh University, Summer Hill, Shimla 171005, Himachal Pradesh, India.
Azmi
W
W
D001711:Biotransformation; D003470:Culture Media; D005285:Fermentation; D007218:Industrial Microbiology / Q000379:methods*; D013294:Lactococcus lactis / Q000201:enzymology* / Q000254:growth & development*; D014439:Tyramine / Q000096:biosynthesis*; D014443:Tyrosine / Q000378:metabolism*; D014445:Tyrosine Decarboxylase / Q000378:metabolism
D003470:Culture Media; D014443:Tyrosine; D014445:Tyrosine Decarboxylase; D014439:Tyramine