A highly sensitive fluorescence probe for fast thiol-quantification assay of glutathione reductase.
Fast detection of cellular thiols in aqueous medium was achieved using a newly developed fluorescence probe (see picture). Based on this probe, a high-throughput fluorescence assay for glutathione reductase was developed.
Yi
Long
L
Department of Chemical Biology and State Key Laboratory of Elemento-organic Chemistry, Nankai University, Tianjin, 300071, China.
Li
Heyang
H
Sun
Lu
L
Liu
Liangliang
L
Zhang
Caihong
C
Xi
Zhen
Z
D002460:Cell Line; D003374:Coumarins / Q000138:chemical synthesis / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry*; D005470:Fluorometry / Q000379:methods; D005980:Glutathione Reductase / Q000032:analysis*; D006801:Humans; D013438:Sulfhydryl Compounds / Q000032:analysis*
D003374:Coumarins; D005456:Fluorescent Dyes; D013438:Sulfhydryl Compounds; D005980:Glutathione Reductase