title: Monte Carlo modeling of multilayer phantoms with multiple fluorophores: simulation algorithm and experimental validation.
abstract: This work is first a description of a statistical simulation algorithm developed for simulating the spectral absorption and emission of several fluorophores in an absorbing and diffusing multilayer model. Second, a detailed experimental validation of the simulation program is conducted on two sets of liquid and solid multilayer phantoms, containing one, two, or three fluorophores, within absorbing and scattering media. Experimental spatially resolved reflectance spectra are acquired in the wavelength band 400 to 800 nm and compared to corresponding simulated spectra. The degree of similarity between experimentation and simulation data is quantified. The results obtained underline good correlations with mean errors varying from 2 to 10%, depending on the number of layers and on the complexity of the phantom's composition.
authors.affiliation: Nancy University, Automatic Control Research Centre (CRAN), Joint Research Unit (UMR) 7039 Nancy University, National Center for Scientific Research (CNRS), F-54516 Vandoeuvre-les-Nancy, France. emilie.pery@ensem.inpl-nancy.fr
authors.forename: Emilie
authors.initials: E
authors.lastname: Péry
authors.forename: Walter C P M
authors.initials: WC
authors.lastname: Blondel
authors.forename: Cédric
authors.initials: C
authors.lastname: Thomas
authors.forename: François
authors.initials: F
authors.lastname: Guillemin
mesh_terms: D000465:Algorithms; D000818:Animals; D003198:Computer Simulation; D006801:Humans; D007089:Image Enhancement / Q000379:methods; D007090:Image Interpretation, Computer-Assisted / Q000379:methods*; D036641:Microscopy, Fluorescence, Multiphoton / Q000295:instrumentation / Q000379:methods*; D008954:Models, Biological; D015233:Models, Statistical; D009010:Monte Carlo Method; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence / Q000295:instrumentation / Q000379:methods*