Avian phospholipid transfer protein causes HDL conversion without affecting cholesterol efflux from macrophages.
Circulatory phospholipid transfer protein (PLTP) has two major functions: 1) transfer of phospholipids towards HDL particles; and 2) modulation of HDL size and composition via the HDL conversion process. In the laying hen (Gallus gallus), the massive oocyte-targeted lipid flow is achieved through the concerted actions of lipases, lipid transfer proteins, and relatives of the LDL receptor family. The aim of the study was to gain insights into the structure and functions of chicken PLTP. The results demonstrate that PLTP is highly conserved from chicken to mammals, as (i) chicken PLTP is associated with plasma HDL; (ii) it clearly possesses phospholipid transfer activity; (iii) it is inactivated at +58 degrees C; and (iv) it mediates conversion of avian and human HDL into small prebeta-mobile HDL and large fused alpha-mobile HDL particles. Our data show that HDL from different chicken models is similar in chemical and physical properties to that of man based on PLTP activity, cholesterol efflux, and HDL conversion assays. In contrast to mammals, PLTP-facilitated HDL remodeling did not enhance cholesterol efflux efficiency of chicken HDL particles.
Saarela
Jani
J
National Institute for Health and Welfare and FIMM, University of Helsinki, Finland. jani.saarela@thl.fi
Metso
Jari
J
Schneider
Wolfgang J
WJ
Jauhiainen
Matti
M
D000818:Animals; D001692:Biological Transport; D002645:Chickens / Q000378:metabolism*; D002784:Cholesterol / Q000097:blood / Q000378:metabolism*; D003001:Cloning, Molecular; D005786:Gene Expression Regulation; D006801:Humans; D007122:Immunoelectrophoresis; D008075:Lipoproteins, HDL / Q000378:metabolism*; D008264:Macrophages / Q000378:metabolism*; D008969:Molecular Sequence Data; D008970:Molecular Weight; D049294:Phospholipid Transfer Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D013997:Time Factors
D008075:Lipoproteins, HDL; D049294:Phospholipid Transfer Proteins; D012333:RNA, Messenger; D002784:Cholesterol