Rapid group-, serotype-, and vaccine strain-specific identification of poliovirus isolates by real-time reverse transcription-PCR using degenerate primers and probes containing deoxyinosine residues.
We have adapted our previously described poliovirus diagnostic reverse transcription-PCR (RT-PCR) assays to a real-time RT-PCR (rRT-PCR) format. Our highly specific assays and rRT-PCR reagents are designed for use in the WHO Global Polio Laboratory Network for rapid and large-scale identification of poliovirus field isolates.
Kilpatrick
David R
DR
Polio and Picornavirus Laboratory Branch, G-10, Division of Viral Diseases, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA. DKilpatrick@cdc.gov
Yang
Chen-Fu
CF
Ching
Karen
K
Vincent
Annelet
A
Iber
Jane
J
Campagnoli
Ray
R
Mandelbaum
Mark
M
De
Lina
L
Yang
Su-Ju
SJ
Nix
Allan
A
Kew
Olen M
OM
D017931:DNA Primers / Q000235:genetics*; D006801:Humans; D015345:Oligonucleotide Probes / Q000235:genetics*; D017955:Poliovirus / Q000145:classification* / Q000302:isolation & purification*; D012367:RNA, Viral / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity
D017931:DNA Primers; D015345:Oligonucleotide Probes; D012367:RNA, Viral