Real time intraoperative confocal laser microscopy-guided surgery.
OBJECTIVE
To assess the potential utility of in vivo histologic surface and subsurface imaging in real-time using the Optiscan confocal laser microscope to detect diseased tissue at the time of surgery.


BACKGROUND
The goal of surgical treatment of diseases such as cancer is complete microscopic resection of diseased tissue; however, current methods for the assessment of extent of disease at the time of surgery are inadequate.


METHODS
We assessed the potential of the Optiscan confocal laser microscope, a new device developed for real-time in vivo histologic surface and subsurface imaging during surgery.


RESULTS
Intravenous Fluorescein Sodium contrast enabled visualization of cellular and architectural morphology of intra-abdominal organs with magnification equivalent to light microscopy and enabled differentiation between normal organs and disease.


CONCLUSIONS
Real time intraoperative confocal microscopy has significant potential application in detecting disease, and influencing decision-making at the time of surgery.
Nguyen
Nam Q
NQ
Cancer Research Program, Garvan Institute of Medical Research, Sydney, New South Wales, Australia.
Biankin
Andrew V
AV
Leong
Rupert W L
RW
Chang
David K
DK
Cosman
Peter H
PH
Delaney
Peter
P
Kench
James G
JG
Merrett
Neil D
ND
D003287:Contrast Media; D004067:Digestive System Neoplasms / Q000473:pathology* / Q000601:surgery*; D019793:Fluorescein; D006801:Humans; D007430:Intraoperative Care; D018613:Microscopy, Confocal
D003287:Contrast Media; D019793:Fluorescein