title: A chemical approach to immunoprotein engineering: chemoselective functionalization of thioester proteins in their native state.
abstract: Less than 6 feet under: Serum proteins C3, C4, and alpha(2)M each contain a thioester domain buried within a hydrophobic pocket, which is thought to shield the labile thioester from hydrolysis. Herein, we make use of the inherent reactivity of the hydrazide for thioester moieties to chemoselectively label these crucial serum regulators in their native conformation; this demonstrates that access to the thioester site is much greater than previously supposed.
authors.affiliation: The Scripps-Oxford Laboratory, Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, UK.
authors.forename: Michael A
authors.initials: MA
authors.lastname: Cole
authors.forename: Sarah E
authors.initials: SE
authors.lastname: Tully
authors.forename: Alister W
authors.initials: AW
authors.lastname: Dodds
authors.forename: James N
authors.initials: JN
authors.lastname: Arnold
authors.forename: Grant E
authors.initials: GE
authors.lastname: Boldt
authors.forename: Robert B
authors.initials: RB
authors.lastname: Sim
authors.forename: John
authors.initials: J
authors.lastname: Offer
authors.forename: Paul
authors.initials: P
authors.lastname: Wentworth
mesh_terms: D001710:Biotin / Q000737:chemistry; D003176:Complement C3 / Q000737:chemistry* / Q000276:immunology; D015935:Complement C4b / Q000737:chemistry* / Q000276:immunology; D005456:Fluorescent Dyes / Q000737:chemistry; D010455:Peptides / Q000737:chemistry; D015202:Protein Engineering; D013438:Sulfhydryl Compounds / Q000737:chemistry*; D000511:alpha-Macroglobulins / Q000737:chemistry* / Q000276:immunology
chemical_list: D003176:Complement C3; D005456:Fluorescent Dyes; D010455:Peptides; D013438:Sulfhydryl Compounds; D000511:alpha-Macroglobulins; D001710:Biotin; D015935:Complement C4b