title: The apoptosis linked gene ALG-2 is dysregulated in tumors of various origin and contributes to cancer cell viability.
abstract: The apoptosis linked gene-2 (ALG-2), discovered as a proapoptotic calcium binding protein, has recently been found upregulated in lung cancer tissue indicating that this protein may play a role in the pathology of cancer cells and/or may be a tumor marker. Using immunohistochemistry on tissue microarrays we analysed the expression of ALG-2 in 7371 tumor tissue samples of various origin as well as in 749 normal tissue samples. Most notably, ALG-2 was upregulated in mesenchymal tumors. No correlation was found between ALG-2 staining intensity and survival of patients with lung, breast or colon cancer. siRNA mediated ALG-2 downregulation led to a significant reduction in viability of HeLa cells indicating that ALG-2 may contribute to tumor development and expansion.
authors.affiliation: Copenhagen Biocenter, Department of Molecular Biology, University of Copenhagen, Ole Maaløes Vej 5, Copenhagen N 2200, Denmark.
authors.forename: Jonas M
authors.initials: JM
authors.lastname: la Cour
authors.forename: Berit R
authors.initials: BR
authors.lastname: Høj
authors.forename: Jens
authors.initials: J
authors.lastname: Mollerup
authors.forename: Ronald
authors.initials: R
authors.lastname: Simon
authors.forename: Guido
authors.initials: G
authors.lastname: Sauter
authors.forename: Martin W
authors.initials: MW
authors.lastname: Berchtold
mesh_terms: D017209:Apoptosis / Q000235:genetics*; D051017:Apoptosis Regulatory Proteins / Q000235:genetics*; D001943:Breast Neoplasms; D002135:Calcium-Binding Proteins / Q000235:genetics*; D002470:Cell Survival / Q000235:genetics; D003110:Colonic Neoplasms; D015536:Down-Regulation / Q000187:drug effects; D015972:Gene Expression Regulation, Neoplastic; D006367:HeLa Cells; D006801:Humans; D008175:Lung Neoplasms; D008648:Mesoderm / Q000473:pathology; D009369:Neoplasms / Q000235:genetics / Q000473:pathology*; D034741:RNA, Small Interfering / Q000494:pharmacology; D046888:Tissue Array Analysis
chemical_list: D051017:Apoptosis Regulatory Proteins; D002135:Calcium-Binding Proteins; C097753:PDCD6 protein, human; D034741:RNA, Small Interfering