[Serum activity of chitotriosidase, lysozyme and cathepsin H in patients with lung cancer and patients with inflammatory exudate (preliminary report)].
Lung cancer remains the leading cause of cancer death over the world. Although new diagnostic methods have been discovered, new biomarkers of the cancer are still under studying. A human chitinolytic enzyme called chitotriosidase hydrolyzes chitin and chitotrioside substrates. It is specifically expressed by activating macrophages and seems to play a role in the defense against chitinous human pathogens. Recently it has been shown that chitotriosidase may also attend to the inflammatory process. The aim of the study was to determine chitotriosidase activity in serum of patients with lung cancer and patients with inflammatory exudate. We studied the usefulness of the above parameter determination in differentiation between lung cancer and inflammation. In addition, serum activity of lysozyme and cathepsin H was determined.


METHODS
The study included 17 patients with inflammatory pleural exudate--group 1., 40 lung cancer patients with malignant pleural effusion--group 2. and 37 healthy subjects. All the patients of group 2. were divided into 2 subgroups: 2A without metastases (n = 23) and 2B with metastases (n = 17). Chitotriosidase and cathepsin H activity was determined in serum by a fluorometric methods. Serum lysozyme activity was measured by turbidimetric method with Micrococcus luteus as substrate.


RESULTS
We observed an increase of the chitotriosidase activity in serum patients of both studied group in comparison with the control. The activity of the chitotriosidase in lung cancer patients was significantly higher than in the control (36.7 vs 68.1 nmol/ml/h; p < 0.01). There were no significant differences in serum lysozyme and cathepsin H activity in patients in comparison to healthy subjects.


CONCLUSIONS
The results suggest that activity of the chitotriosidase can not be used to differentiation between inflammation and cancer in lung.
Gołab
Krzysztof
K
Department of Pharmaceutical Biochemistry, Medical University of Wroclaw, Poland. golab@biochfarm.am.wroc.pl
Passowicz-Muszyńska
Ewa
E
Jankowska
Renata
R
Warwas
Maria
M
D000368:Aged; D014408:Biomarkers, Tumor / Q000097:blood*; D056655:Cathepsin H; D002403:Cathepsins / Q000097:blood*; D003546:Cysteine Endopeptidases / Q000097:blood*; D003937:Diagnosis, Differential; D005260:Female; D006596:Hexosaminidases / Q000097:blood*; D006801:Humans; D008175:Lung Neoplasms / Q000175:diagnosis / Q000201:enzymology*; D008297:Male; D009113:Muramidase / Q000097:blood*; D010996:Pleural Effusion / Q000175:diagnosis / Q000201:enzymology*; D016066:Pleural Effusion, Malignant / Q000175:diagnosis / Q000201:enzymology; D010998:Pleurisy / Q000175:diagnosis / Q000201:enzymology*
D014408:Biomarkers, Tumor; D006596:Hexosaminidases; C086400:chitotriosidase; D009113:Muramidase; D002403:Cathepsins; D003546:Cysteine Endopeptidases; C534126:CTSH protein, human; D056655:Cathepsin H