title: Mycorrhizal synthesis of Tuber indicum with two indigenous hosts, Castanea mollissima and Pinus armandii.
abstract: Tuber indicum is one of the most renowned commercialized fungi in China. Mycorrhizal investigations, however, have been carried out mainly with exotic trees. Up to now there is no detailed description of morphology of the mycorrhizae formed with the indigenous hosts of T. indicum. Containerized seedlings of two indigenous hosts of the fungus in southwestern China, Pinus armandii and Castanea mollissima, were inoculated with aqueous spore suspension of T. indicum in two kinds of substrates. Mycorrhizae began to form 4 months after inoculation and were harvested at 9 months. The contributing fungus of the mycorrhizae was confirmed to be T. indicum by morphological and ITS-rDNA sequence analyses. The morphology of emanating hyphae and epidermoid-like mantle appearance was similar to the mycorrhizae obtained with some European trees. The high morphological variation and the similarity to that of Tuber melanosporum makes it difficult to distinguish the mycorrhizae of the two species by morphology alone. The synthesis of mycorrhizae of T. indicum with its indigenous hosts will be of great significance for planned cultivation of the Asian black truffles.
authors.affiliation: Key Laboratory of Biodiversity and Biogeography, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, 650204, People's Republic of China.|College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou, 730020, People's Republic of China.
authors.forename: Li-Ying
authors.initials: LY
authors.lastname: Geng
authors.affiliation: Key Laboratory of Biodiversity and Biogeography, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, 650204, People's Republic of China.
authors.forename: Xiang-Hua
authors.initials: XH
authors.lastname: Wang
authors.affiliation: Key Laboratory of Biodiversity and Biogeography, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, 650204, People's Republic of China.
authors.forename: Fu-Qiang
authors.initials: FQ
authors.lastname: Yu
authors.affiliation: Key Laboratory of Biodiversity and Biogeography, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, 650204, People's Republic of China.|Graduate University of Chinese Academy of Sciences, Beijing, 100049, People's Republic of China.
authors.forename: Xiao-Juan
authors.initials: XJ
authors.lastname: Deng
authors.affiliation: Key Laboratory of Biodiversity and Biogeography, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, 650204, People's Republic of China.|Graduate University of Chinese Academy of Sciences, Beijing, 100049, People's Republic of China.
authors.forename: Xiao-Fei
authors.initials: XF
authors.lastname: Tian
authors.affiliation: Key Laboratory of Biodiversity and Biogeography, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, 650204, People's Republic of China.|Graduate University of Chinese Academy of Sciences, Beijing, 100049, People's Republic of China.
authors.forename: Xiao-Fei
authors.initials: XF
authors.lastname: Shi
authors.affiliation: Key Laboratory of Biodiversity and Biogeography, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, 650204, People's Republic of China.|Graduate University of Chinese Academy of Sciences, Beijing, 100049, People's Republic of China.
authors.forename: Xue-Dan
authors.initials: XD
authors.lastname: Xie
authors.affiliation: Key Laboratory of Biodiversity and Biogeography, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, 650204, People's Republic of China. pgliu@mail.kib.ac.cn.
authors.forename: Pei-Gui
authors.initials: PG
authors.lastname: Liu
authors.affiliation: College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou, 730020, People's Republic of China. yy.shen@lzu.edu.cn.
authors.forename: Yu-Ying
authors.initials: YY
authors.lastname: Shen
mesh_terms: D001203:Ascomycota / Q000166:cytology / Q000235:genetics / Q000254:growth & development* / Q000302:isolation & purification; D029962:Fagaceae / Q000382:microbiology*; D038821:Mycorrhizae / Q000166:cytology / Q000235:genetics / Q000254:growth & development* / Q000302:isolation & purification; D028223:Pinus / Q000382:microbiology*