title: A vaccine against S. pyogenes: design and experimental immune response.
abstract: Streptococcus pyogenes causes severe invasive infections: the post-streptococcal sequelae of acute rheumatic fever (RF) and rheumatic heart disease (RHD), acute glomerulonephritis, and uncomplicated pharyngitis and pyoderma. Efforts to produce a vaccine against S. pyogenes began several decades ago, and different models have been proposed. Here, we describe the methodology used in the development of a new vaccine model, consisting of both T and B protective epitopes constructed as synthetic peptides and recombinant proteins. Two adjuvants were tested in an experimental inbred mouse model: a classical Freund's adjuvant and a new adjuvant (AFCo1) that induces mucosal immune responses and is obtained by calcium precipitation of a proteoliposome derived from the outer membrane of Neisseria meningitides B. The StreptInCor vaccine epitope co-administrated with AFCo1 adjuvant induced mucosal (IgA) and systemic (IgG) antibodies as preferential Th1-mediated immune responses. No autoimmune reactions were observed, suggesting that the vaccine epitope is safe.
authors.affiliation: Heart Institute (InCor), School of Medicine, University of São Paulo, Brazil. luizagui@usp.br
authors.forename: L
authors.initials: L
authors.lastname: Guilherme
authors.forename: E
authors.initials: E
authors.lastname: Postol
authors.forename: S
authors.initials: S
authors.lastname: Freschi de Barros
authors.forename: F
authors.initials: F
authors.lastname: Higa
authors.forename: R
authors.initials: R
authors.lastname: Alencar
authors.forename: M
authors.initials: M
authors.lastname: Lastre
authors.forename: C
authors.initials: C
authors.lastname: Zayas
authors.forename: C R
authors.initials: CR
authors.lastname: Puschel
authors.forename: W R
authors.initials: WR
authors.lastname: Silva
authors.forename: L C
authors.initials: LC
authors.lastname: Sa-Rocha
authors.forename: V M
authors.initials: VM
authors.lastname: Sa-Rocha
authors.forename: O
authors.initials: O
authors.lastname: Pérez
authors.forename: J
authors.initials: J
authors.lastname: Kalil
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D015195:Drug Design; D005260:Female; D018928:Immunity, Mucosal / Q000187:drug effects; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D022541:Streptococcal Vaccines / Q000008:administration & dosage / Q000138:chemical synthesis / Q000276:immunology*; D013297:Streptococcus pyogenes / Q000187:drug effects / Q000276:immunology*
chemical_list: D022541:Streptococcal Vaccines