Simultaneous determination of five coumarins in Angelicae dahuricae Radix by HPLC/UV and LC-ESI-MS/MS.
Rapid, simple and reliable HPLC/UV and LC-ESI-MS/MS methods for the simultaneous determination of five active coumarins of Angelicae dahuricae Radix, byakangelicol (1), oxypeucedanin (2), imperatorin (3), phellopterin (4) and isoimperatorin (5) were developed and validated. The separation condition for HPLC/UV was optimized using a Develosil RPAQUEOUS C(30) column using 70% acetonitrile in water as the mobile phase. This HPLC/UV method was successful for providing the baseline separation of the five coumarins with no interfering peaks detected in the 70% ethanol extract of Angelicae dahuricae Radix. The specific determination of the five coumarins was also accomplished by a triple quadrupole tandem mass spectrometer equipped with an electrospray ionization source (LC-ESI-MS/MS). Multiple reaction monitoring (MRM) in the positive mode was used to enhance the selectivity of detection. The LC-ESI-MS/MS methods were successfully applied for the determination of the five major coumarins in Angelicae dahuricae Radix. These HPLC/UV and LC-ESI-MS/MS methods were validated in terms of recovery, linearity, accuracy and precision (intra- and inter-day validation). Taken together, the shorter analysis time involved makes these HPLC/UV and LC-ESI-MS/MS methods valuable for the commercial quality control of Angelicae dahuricae Radix extracts and its pharmaceutical preparations.
Park
Ah Yeon
AY
Department of Pharmaceutical Analysis, College of Pharmacy, Chung-Ang University, 221 Heuksuk-Dong, Dongjak-Ku, Seoul 156-756, Korea.
Park
So-Young
SY
Lee
Jaehyun
J
Jung
Mihye
M
Kim
Jinwoong
J
Kang
Sam Sik
SS
Youm
Jeong-Rok
JR
Han
Sang Beom
SB
D029969:Angelica / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003374:Coumarins / Q000032:analysis*; D005663:Furans / Q000032:analysis; D011564:Furocoumarins / Q000032:analysis; D016014:Linear Models; D018517:Plant Roots / Q000737:chemistry; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet / Q000379:methods; D053719:Tandem Mass Spectrometry / Q000379:methods*; D013696:Temperature
D003374:Coumarins; D005663:Furans; D011564:Furocoumarins; C055542:isoimperatorin; C104601:phellopterin; C462034:byakangelicol; C031534:imperatorin; C031535:oxypeucadanin