title: Biosensing by optical waveguide spectroscopy based on localized surface plasmon resonance of gold nanoparticles used as a probe or as a label.
abstract: The application of localized surface plasmon resonance (LSPR) of gold nanoparticles for the detection of biotin-streptavidin binding, as a typical biological reaction, was investigated by using optical waveguide spectroscopy, and two different modes for the use of gold nanoparticles, one as a probe and the other as a label were compared with each other. The combination with optical waveguide spectroscopy was found to bring about a high sensitivity for the biomolecular detection system using LSPR of gold nanoparticles in both modes. In particular, the mode using gold nanoparticles as a label was demonstrated to be of advantage to devising proper procedures for using nanoparticles and evaluating actual response relevant to the phenomenon concerned, and thus to sensitive detection.
authors.affiliation: Consolidated Research Institute for Advanced Science and Medical Care, Waseda University, 3-4-1 Okubo, Shinjuku-ku, Tokyo 169-8555, Japan.
authors.forename: Mayuki
authors.initials: M
authors.lastname: Kajiura
authors.forename: Takuya
authors.initials: T
authors.lastname: Nakanishi
authors.forename: Hironori
authors.initials: H
authors.lastname: Iida
authors.forename: Harumi
authors.initials: H
authors.lastname: Takada
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Osaka
mesh_terms: D015374:Biosensing Techniques; D001710:Biotin / Q000378:metabolism; D006046:Gold / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry*; D011485:Protein Binding; D012680:Sensitivity and Specificity; D019809:Streptavidin / Q000032:analysis* / Q000378:metabolism; D020349:Surface Plasmon Resonance / Q000295:instrumentation / Q000379:methods*
chemical_list: D001710:Biotin; D006046:Gold; D019809:Streptavidin