title: Dietary omega-3 fatty acids attenuate cellular damage after a hippocampal ischemic insult in adult rats.
abstract: The role of omega-3 polyunsaturated fatty acids (3PUFAs) on brain function is increasingly demonstrated. Here, the effect of dietary deprivation of essential 3PUFAs on some parameters related to neuroprotection was investigated. Rats were fed with two different diets: omega-3 diet and omega-3-deprived diet. To assess the influence of 3PUFAs on brain responses to ischemic insult, hippocampal slices were subjected to an oxygen and glucose deprivation (OGD) model of in vitro ischemia. The omega-3-deprived group showed higher cell damage and stronger decrease in the [(3)H]glutamate uptake after OGD. Moreover, omega-3 deprivation influenced antiapoptotic cell response after OGD, affecting GSK-3beta and ERK1/2, but not Akt, phosphorylation. Taken together, these results suggest that 3PUFAs are important for cell protection after ischemia and also seem to play an important role in the activation of antiapoptotic signaling pathways.
authors.affiliation: Biochemistry Department, Biochemistry Postgraduate Program, ICBS, Federal University of Rio Grande do Sul, 90035-003 Porto Alegre, RS, Brazil. juliamoreira@gmail.com.br
authors.forename: Júlia D
authors.initials: JD
authors.lastname: Moreira
authors.forename: Luisa
authors.initials: L
authors.lastname: Knorr
authors.forename: Ana Paula
authors.initials: AP
authors.lastname: Thomazi
authors.forename: Fabrício
authors.initials: F
authors.lastname: Simão
authors.forename: Cíntia
authors.initials: C
authors.lastname: Battú
authors.forename: Jean Pierre
authors.initials: JP
authors.lastname: Oses
authors.forename: Carmem
authors.initials: C
authors.lastname: Gottfried
authors.forename: Susana
authors.initials: S
authors.lastname: Wofchuk
authors.forename: Christianne
authors.initials: C
authors.lastname: Salbego
authors.forename: Diogo O
authors.initials: DO
authors.lastname: Souza
authors.forename: Marcos L S
authors.initials: ML
authors.lastname: Perry
authors.forename: Lúcia
authors.initials: L
authors.lastname: Vinadé
mesh_terms: D000818:Animals; D016923:Cell Death; D015687:Cell Hypoxia; D004042:Dietary Fats, Unsaturated / Q000008:administration & dosage*; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism; D005228:Fatty Acids, Essential / Q000172:deficiency; D015525:Fatty Acids, Omega-3 / Q000008:administration & dosage* / Q000502:physiology; D005260:Female; D038362:Glycogen Synthase Kinase 3 / Q000378:metabolism; D000071679:Glycogen Synthase Kinase 3 beta; D006624:Hippocampus / Q000378:metabolism / Q000503:physiopathology*; D020925:Hypoxia-Ischemia, Brain / Q000378:metabolism / Q000503:physiopathology*; D066298:In Vitro Techniques; D008297:Male; D018696:Neuroprotective Agents / Q000008:administration & dosage*; D010766:Phosphorylation; D011499:Protein Processing, Post-Translational; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000378:metabolism / Q000517:prevention & control*; D015398:Signal Transduction
chemical_list: D004042:Dietary Fats, Unsaturated; D005228:Fatty Acids, Essential; D015525:Fatty Acids, Omega-3; D018696:Neuroprotective Agents; D000071679:Glycogen Synthase Kinase 3 beta; C000605508:Gsk3b protein, rat; D048049:Extracellular Signal-Regulated MAP Kinases; D038362:Glycogen Synthase Kinase 3