title: Vdelta1 T lymphocytes producing IFN-gamma and IL-17 are expanded in HIV-1-infected patients and respond to Candida albicans.
abstract: In early HIV-1 infection, Vdelta1 T lymphocytes are increased in peripheral blood and this is related to chemokine receptor expression, chemokine response, and recirculation. Herein we show that, at variance with healthy donors, in HIV-1-infected patients ex vivo-isolated Vdelta1 T cells display cytoplasmic interferon-gamma (IFN-gamma). Interestingly, these cells coexpress cytoplasmic interleukin-17 (IL-17), and bear the CD27 surface marker of the memory T-cell subset. Vdelta1 T cells, isolated from either patients or healthy donors, can proliferate and produce IFN-gamma and IL-17 in response to Candida albicans in vitro, whereas Vdelta2 T cells respond with proliferation and IFN-gamma/IL-17 production to mycobacterial or phosphate antigens. These IFN-gamma/IL-17 double-producer gammadelta T cells express the Th17 RORC and the Th1 TXB21 transcription factors and bear the CCR7 homing receptor and the CD161 molecule that are involved in gammadelta T-cell transendothelial migration. Moreover, Vdelta1 T cells responding to C albicans express the chemokine receptors CCR4 and CCR6. This specifically equipped circulating memory gammadelta T-cell population might play an important role in the control of HIV-1 spreading and in the defense against opportunistic infections, possibly contributing to compensate for the impairment of CD4(+) T cells.
authors.affiliation: Centre of Excellence for Biological Sciences, University of Genoa, Genoa, Italy.
authors.forename: Daniela
authors.initials: D
authors.lastname: Fenoglio
authors.forename: Alessandro
authors.initials: A
authors.lastname: Poggi
authors.forename: Silvia
authors.initials: S
authors.lastname: Catellani
authors.forename: Florinda
authors.initials: F
authors.lastname: Battaglia
authors.forename: Alessandra
authors.initials: A
authors.lastname: Ferrera
authors.forename: Maurizio
authors.initials: M
authors.lastname: Setti
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Murdaca
authors.forename: Maria Raffaella
authors.initials: MR
authors.lastname: Zocchi
mesh_terms: D000328:Adult; D002176:Candida albicans / Q000276:immunology*; D005260:Female; D016694:Gene Rearrangement, delta-Chain T-Cell Antigen Receptor; D015658:HIV Infections / Q000276:immunology*; D015497:HIV-1; D006801:Humans; D007371:Interferon-gamma / Q000096:biosynthesis*; D020381:Interleukin-17 / Q000096:biosynthesis* / Q000235:genetics; D018655:Lymphocyte Count; D008297:Male; D008875:Middle Aged; D055651:NK Cell Lectin-Like Receptor Subfamily B / Q000032:analysis; D057132:Nuclear Receptor Subfamily 1, Group F, Member 3; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000235:genetics / Q000276:immunology*; D054398:Receptors, CCR4 / Q000032:analysis; D054399:Receptors, CCR6 / Q000032:analysis; D054400:Receptors, CCR7 / Q000032:analysis; D018168:Receptors, Retinoic Acid / Q000032:analysis; D011988:Receptors, Thyroid Hormone / Q000032:analysis; D016176:T-Lymphocyte Subsets / Q000276:immunology* / Q000378:metabolism; D014157:Transcription Factors / Q000032:analysis; D055815:Young Adult
chemical_list: C515354:CCR4 protein, human; C106311:CCR6 protein, human; C515370:CCR7 protein, human; D020381:Interleukin-17; C527203:KLRB1 protein, human; D055651:NK Cell Lectin-Like Receptor Subfamily B; D057132:Nuclear Receptor Subfamily 1, Group F, Member 3; C539900:RORC protein, human; D016692:Receptors, Antigen, T-Cell, gamma-delta; D054398:Receptors, CCR4; D054399:Receptors, CCR6; D054400:Receptors, CCR7; D018168:Receptors, Retinoic Acid; D011988:Receptors, Thyroid Hormone; D014157:Transcription Factors; D007371:Interferon-gamma