title: Dengue virus serotype 2 from a sylvatic lineage isolated from a patient with dengue hemorrhagic fever.
abstract: Dengue viruses circulate in both human and sylvatic cycles. Although dengue viruses (DENV) infecting humans can cause major epidemics and severe disease, relatively little is known about the epidemiology and etiology of sylvatic dengue viruses. A 20-year-old male developed dengue hemorrhagic fever (DHF) with thrombocytopenia (12,000/ul) and a raised hematocrit (29.5% above baseline) in January 2008 in Malaysia. Dengue virus serotype 2 was isolated from his blood on day 4 of fever. A phylogenetic analysis of the complete genome sequence revealed that this virus was a member of a sylvatic lineage of DENV-2 and most closely related to a virus isolated from a sentinel monkey in Malaysia in 1970. This is the first identification of a sylvatic DENV circulating in Asia since 1975.
authors.affiliation: Institute of Health & Community Medicine, Universiti Malaysia Sarawak, Kota Samarahan, Sarawak, Malaysia. jane.cardosa@gmail.com
authors.forename: Jane
authors.initials: J
authors.lastname: Cardosa
authors.forename: Mong How
authors.initials: MH
authors.lastname: Ooi
authors.forename: Phaik Hooi
authors.initials: PH
authors.lastname: Tio
authors.forename: David
authors.initials: D
authors.lastname: Perera
authors.forename: Edward C
authors.initials: EC
authors.lastname: Holmes
authors.forename: Khatijar
authors.initials: K
authors.lastname: Bibi
authors.forename: Zahara
authors.initials: Z
authors.lastname: Abdul Manap
mesh_terms: D000818:Animals; D001769:Blood / Q000821:virology; D016000:Cluster Analysis; D003716:Dengue Virus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D016679:Genome, Viral; D006801:Humans; D008296:Malaysia; D008297:Male; D008969:Molecular Sequence Data; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D019595:Severe Dengue / Q000473:pathology / Q000821:virology*; D055815:Young Adult