The selection of single-chain Fv antibody fragments specific to Bhlp 29.7 protein of Brachyspira hyodysenteriae.
Single-chain antibodies (scFv) specific to Brachyspira hyodysenteriae were isolated from a phagemid library. Recombinant Bhlp 29.7 protein was used for scFv selection and individual clones were tested by ELISA and immunofluorescent test; four unique clones were isolated. One of selected clones was able to bind specifically B. hyodysenteriae in ELISA and immunofluorescence test. This is the first report of species-specific recombinant antibodies against B. hyodysenteriae.
Lobová
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Institute of Microbiology and Immunology, Veterinary and Pharmaceutical University Brno, Czech Republic.
Cízek
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Celer
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D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology*; D000911:Antibodies, Monoclonal / Q000276:immunology; D000918:Antibody Specificity; D000942:Antigens, Bacterial / Q000276:immunology*; D001426:Bacterial Proteins / Q000276:immunology*; D017401:Brachyspira hyodysenteriae / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D019085:Fluorescent Antibody Technique, Direct; D016905:Gram-Negative Bacterial Infections / Q000175:diagnosis / Q000662:veterinary; D007135:Immunoglobulin Variable Region / Q000276:immunology*; D008074:Lipoproteins / Q000276:immunology*; D019151:Peptide Library; D011993:Recombinant Fusion Proteins / Q000276:immunology; D013045:Species Specificity; D013552:Swine; D013553:Swine Diseases / Q000175:diagnosis
D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D007135:Immunoglobulin Variable Region; D008074:Lipoproteins; D019151:Peptide Library; D011993:Recombinant Fusion Proteins