Cigarette smoke regulates the expression of TLR4 and IL-8 production by human macrophages.
BACKGROUND
Toll-like receptors (TLRs) are present on monocytes and alveolar macrophages that form the first line of defense against inhaled particles. The importance of those cells in the pathophysiology of chronic obstructive pulmonary disease (COPD) has well been documented. Cigarette smoke contains high concentration of oxidants which can stimulate immune cells to produce reactive oxygen species, cytokines and chemokines.


METHODS
In this study, we evaluated the effects of cigarette smoke medium (CSM) on TLR4 expression and interleukin (IL)-8 production by human macrophages investigating the involvement of ROS.


CONCLUSIONS
TLR4 surface expression was downregulated on short term exposure (1 h) of CSM. The downregulation could be explained by internalization of the TLR4 and the upregulation by an increase in TLR4 mRNA. IL-8 mRNA and protein were also increased by CSM. CSM stimulation increased intracellular ROS-production and decreased glutathione (GSH) levels. The modulation of TLR4 mRNA and surface receptors expression, IRAK activation, IkappaB-alpha degradation, IL-8 mRNA and protein, GSH depletion and ROS production were all prevented by antioxidants such as N-acetyl-L-cysteine (NAC).


CONCLUSIONS
TLR4 may be involved in the pathogenesis of lung emphysema and oxidative stress and seems to be a crucial contributor in lung inflammation.
Sarir
Hadi
H
Division of Pharmacology and Pathophysiology, Departement of Pharmaceutical Sciences, Faculty of Sciences, Utrecht University, the Netherlands. e.mortaz@uu.nl.
Mortaz
Esmaeil
E
Karimi
Khalil
K
Kraneveld
Aletta D
AD
Rahman
Irfan
I
Caldenhoven
Eric
E
Nijkamp
Frans P
FP
Folkerts
Gert
G