Establishment and characterization of baboon embryonic stem cell lines: an Old World Primate model for regeneration and transplantation research.
Here we have developed protocols using the baboon as a complementary alternative Old World Primate to rhesus and other macaques which have severe limitations in their availability. Baboons are not limited as research resources, they are evolutionarily closer to humans, and the multiple generations of pedigreed colonies which display complex human disease phenotypes all support their further optimization as an invaluable primate model. Since neither baboon-assisted reproductive technologies nor baboon embryonic stem cells (ESCs) have been reported, here we describe the first derivations and characterization of baboon ESC lines from IVF-generated blastocysts. Two ESCs lines (BabESC-4 and BabESC-15) display ESC morphology, express pluripotency markers (Oct-4, hTert, Nanog, Sox-2, Rex-1, TRA1-60, TRA1-81), and maintain stable euploid female karyotypes with parentage confirmed independently. They have been grown continuously for >430 and 290 days, respectively. Teratomas from both lines have all three germ layers. Availabilities of these BabESCs represent another important resource for stem cell biologists.
Simerly
Calvin R
CR
Division of Developmental and Regenerative Medicine, Department of Obstetrics, Gynecology, and Reproductive Sciences, University of Pittsburgh, Pittsburgh, PA 15213, USA.
Navara
Christopher S
CS
Castro
Carlos A
CA
Turpin
Janet C
JC
Redinger
Carrie J
CJ
Mich-Basso
Jocelyn D
JD
Jacoby
Ethan S
ES
Grund
Kevin J
KJ
McFarland
David A
DA
Oliver
Stacie L
SL
Ben-Yehudah
Ahmi
A
Carlisle
Diane L
DL
Frost
Patricia
P
Penedo
Cecilia
C
Hewitson
Laura
L
Schatten
Gerald
G
D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D001757:Blastomeres / Q000166:cytology; D002454:Cell Differentiation; D002460:Cell Line; D053595:Embryonic Stem Cells / Q000166:cytology* / Q000378:metabolism / Q000637:transplantation; D005260:Female; D018398:Homeodomain Proteins / Q000235:genetics / Q000378:metabolism; D007150:Immunohistochemistry; D007621:Karyotyping; D051741:Kruppel-Like Transcription Factors / Q000235:genetics / Q000378:metabolism; D008954:Models, Biological; D050814:Octamer Transcription Factor-3 / Q000235:genetics / Q000378:metabolism; D010215:Papio; D011323:Primates; D044968:Regenerative Medicine; D055748:SOXB1 Transcription Factors / Q000235:genetics / Q000378:metabolism; D019098:Telomerase / Q000235:genetics / Q000378:metabolism
D015415:Biomarkers; D018398:Homeodomain Proteins; D051741:Kruppel-Like Transcription Factors; D050814:Octamer Transcription Factor-3; D055748:SOXB1 Transcription Factors; D019098:Telomerase