[New method of "ingredient difference phonetypical cloning" for functional gene cloning from medicinal plants].
This paper introduced a new method of "ingredient difference phonetypical cloning" for functional gene clone of medicinal plants, which might solve the difficulties in isolating genes encoding enzymes for the biosynthesis of secondary metabolites by usual ways. Concepts, mechanisms and methods were systematically introduced and possibility was proved by experiments. The method showed the extra superiority of for the isolation of the genes belonged to unknown metabolic pathway and little information about its sequences. The method provides a new way to isolate functional gene cloning from Chinese herbs and a fundament for the further study on medicinal plant genetic engineering.
Wang
Xueyong
X
College of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, China.
Cui
Guanghong
G
Gao
Wei
W
Huang
Luqi
L
D003001:Cloning, Molecular; D004365:Drugs, Chinese Herbal / Q000378:metabolism; D017343:Genes, Plant / Q000235:genetics*; D005818:Genetic Engineering / Q000379:methods*; D010641:Phenotype; D010946:Plants, Medicinal / Q000235:genetics* / Q000378:metabolism; D015203:Reproducibility of Results; D013997:Time Factors
D004365:Drugs, Chinese Herbal