Analysis of reconstituted chromatin using a solid-phase approach.
Complex experimental strategies involving in vitro reconstituted chromatin or simple chromatin interaction studies are much facilitated by immobilizing the nucleosomal arrays to paramagnetic beads. Chromatin-containing beads can be retrieved from a reaction mix solution on a magnet fast and quantitatively, effectively separating bound, loosely attached and unbound components efficiently. This chapter details a convenient strategy for immobilization of linear plasmid DNA on streptavidin-coated beads, the reconstitution of chromatin on such beads and some fundamental handling procedures.
Sandaltzopoulos
Raphael
R
Laboratory of Gene Expression, Molecular Diagnostics and Modern Therapeutics, Department of Molecular Biology and Genetics, Democritus University of Thrace, Alexandroupolis, Greece.
Becker
Peter B
PB
D000818:Animals; D002843:Chromatin / Q000378:metabolism*; D004247:DNA / Q000378:metabolism; D003956:Dialysis; D004331:Drosophila melanogaster / Q000378:metabolism; D008967:Molecular Biology / Q000379:methods*
D002843:Chromatin; D004247:DNA