Octadecylsilyl silica column method for extraction of messenger RNA.
We have developed a simple and rapid method for the purification of poly(A) tail-messenger RNA (mRNA) from total RNA by using a solid phase extraction column filled with a small amount of octadecylsilyl silica. The method is based on a hydrophobic interaction between the poly(A) tail and the octadecyl unit on the silica particle in a water/dimethyl sulfoxide mixed solution. By using this column, mRNA can be separated from 100 microg of total RNA in less than 10 min with high yields (>80%).
Kimura
Taro
T
Fukuoka Industrial Technology Center, Biotechnology and Food Research Institute, Kurume, Fukuoka 839-0861, Japan. taro@fitc.pref.fukuoka.jp
Sakurai
Kazuo
K
Shinkai
Seiji
S
D057927:Hydrophobic and Hydrophilic Interactions; D012333:RNA, Messenger / Q000302:isolation & purification*; D012822:Silicon Dioxide / Q000737:chemistry*; D052616:Solid Phase Extraction / Q000379:methods*; D013997:Time Factors
D012333:RNA, Messenger; C026679:octadecylsilica; D012822:Silicon Dioxide