title: [Comparison of the mechanisms of intralesional steroid, interferon or verapamil injection in the treatment of proliferative scars].
abstract: OBJECTIVE
  To investigate the effects of intralesional steroid, interferon alpha-2b or verapamil injection on proliferation, apoptosis and TGF-beta1 expression in keloid and hypertrophic scar in vivo.
  
  
  METHODS
  6 patients with keloids and 6 patients with hypertrophic scar were treated with intralesional injection of triamcinolone acetonide (40 mg/ml) or IFN alpha-2b (15 x 10(5) U/ml) or verapamil (2.5 mg/ml). Samples were collected on the 7th day after intralesional injection. Samples of untreated keloid and hypertrophic scar and normal skin were used as control. Expression of PCNA and TGF-beta1 was detected in situ by immunohistochemical staining, and apoptosis was detected in situ by terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate-biotin nick end labeling (TUNEL).
  
  
  RESULTS
  1) Triamcinolone acetonide could prohibit proliferative scars through inhibiting cell proliferation and TGF-beta1 expression, as well as inducing apoptosis. 2) IFN alpha-2b could prohibit proliferative scars through inhibiting cell proliferation and TGF-beta1 expression, but not inducing apoptosis; 3) Verapamil could also prohibit proliferative scars through inhibiting proliferation and TGF-beta1 expression in fibroblasts, as well as inducing apoptosis. While the effect of inducing apoptosis was stronger than that of triamcinolone acetonide, the effect of inhibiting TGF-beta1 expression was weaker than those of triamcinolone acetonide and IFN alpha-2b.
  
  
  CONCLUSIONS
  Although intraleional injection of steroid, interferon alpha-2b or verapamil were all effective in the treatment of keloid and hypertrophic scar, their mechanisms are not similar.
authors.affiliation: Department of Plastic Surgery of the Affiliated Hospital of Hangzhou Normal University, Hangzhou, China.
authors.forename: Shao-jun
authors.initials: SJ
authors.lastname: Xu
authors.forename: Jian-ying
authors.initials: JY
authors.lastname: Teng
authors.forename: Jing
authors.initials: J
authors.lastname: Xie
authors.forename: Ming-qiang
authors.initials: MQ
authors.lastname: Shen
authors.forename: Dong-ming
authors.initials: DM
authors.lastname: Chen
mesh_terms: D000293:Adolescent; D000328:Adult; D017209:Apoptosis; D002648:Child; D017439:Cicatrix, Hypertrophic / Q000188:drug therapy*; D005260:Female; D006801:Humans; D000077190:Interferon alpha-2; D016898:Interferon-alpha / Q000627:therapeutic use*; D008297:Male; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism; D011994:Recombinant Proteins; D013256:Steroids / Q000627:therapeutic use*; D053773:Transforming Growth Factor beta1 / Q000378:metabolism; D014700:Verapamil / Q000627:therapeutic use*; D055815:Young Adult
chemical_list: D000077190:Interferon alpha-2; D016898:Interferon-alpha; D018809:Proliferating Cell Nuclear Antigen; D011994:Recombinant Proteins; D013256:Steroids; D053773:Transforming Growth Factor beta1; D014700:Verapamil