title: AKINbeta1 is involved in the regulation of nitrogen metabolism and sugar signaling in Arabidopsis.
abstract: Sucrose non-fermenting-1-related protein kinase 1 (SnRK1) has been located at the heart of the control of metabolism and development in plants. The active SnRK1 form is usually a heterotrimeric complex. Subcellular localization and specific target of the SnRK1 kinase are regulated by specific beta subunits. In Arabidopsis, there are at least seven genes encoding beta subunits, of which the regulatory functions are not yet clear. Here, we tried to study the function of one beta subunit, AKINbeta1. It showed that AKINbeta1 expression was dramatically induced by ammonia nitrate but not potassium nitrate, and the investigation of AKINbeta1 transgenic Arabidopsis and T-DNA insertion lines showed that AKINbeta1 negatively regulated the activity of nitrate ruductase and was positively involved in sugar repression in early seedling development. Meanwhile AKINbeta1 expression was reduced upon sugar treatment (including mannitol) and did not affect the activity of sucrose phosphate synthase. The results indicate that AKINbeta1 is involved in the regulation of nitrogen metabolism and sugar signaling.
authors.affiliation: School of Life Sciences, East China Normal University, Shanghai 200062, China. xfli@bio.ecnu.edu.cn
authors.forename: Xiao-Fang
authors.initials: XF
authors.lastname: Li
authors.forename: Yu-Ju
authors.initials: YJ
authors.lastname: Li
authors.forename: Ying-Hui
authors.initials: YH
authors.lastname: An
authors.forename: Li-Jun
authors.initials: LJ
authors.lastname: Xiong
authors.forename: Xing-Hua
authors.initials: XH
authors.lastname: Shao
authors.forename: Yang
authors.initials: Y
authors.lastname: Wang
authors.forename: Yue
authors.initials: Y
authors.lastname: Sun
mesh_terms: D017360:Arabidopsis / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D002241:Carbohydrates / Q000494:pharmacology / Q000502:physiology*; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D004269:DNA, Bacterial / Q000235:genetics; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D005964:Glucosyltransferases / Q000378:metabolism; D050901:Nitrate Reductase / Q000378:metabolism; D009566:Nitrates / Q000494:pharmacology; D009584:Nitrogen / Q000378:metabolism*; D017680:Potassium Compounds / Q000494:pharmacology; D015398:Signal Transduction / Q000187:drug effects / Q000235:genetics; D013395:Sucrose / Q000494:pharmacology
chemical_list: C516466:AKINbeta1 protein, Arabidopsis; D029681:Arabidopsis Proteins; D002241:Carbohydrates; D002352:Carrier Proteins; D004269:DNA, Bacterial; D009566:Nitrates; D017680:Potassium Compounds; C036483:T-DNA; D013395:Sucrose; D050901:Nitrate Reductase; D005964:Glucosyltransferases; C022698:sucrose-phosphate synthase; D009584:Nitrogen; C023844:potassium nitrate; C006568:ammonium nitrate