Effects of branching architecture and linker on the activity of hyperbranched polymer-drug conjugates.
Drug release from hyperbranched polymer-drug conjugates and the subsequent activity are influenced by the branching architecture and the linker. To gain an understanding of these effects, we used hyperbranched polyol and G4-OH polyamidoamine (PAMAM) dendrimer with methyl prednisolone (MP) as the model drug. The drug was conjugated to dendrimer or polyol using a glutaric acid (GA) or a succinic acid (SA) spacer. Drug payload was the highest with polyol, while in the case of dendrimer, a higher payload was achieved with the GA than the SA spacer. Cell uptake of the polymer conjugates in A549 lung epithelial cells was higher than that of the free drug, and the conjugates largely localized in the cytosol. The anti-inflammatory activity of polymer conjugated MP, as measured by inhibition of prostaglandin synthesis, was the highest for MP-SA-dendrimer conjugate, followed by MP-GA-polyol conjugate, and then MP-GA-dendrimer conjugate. This study suggests that the branching architecture and spacer influence the drug payload and pharmacological activity of a drug-nanopolymer conjugate, which may significantly influence the in vivo efficacy of these nanodevices. This has key implications in the eventual in vivo efficacy of these nanodevices.
Perumal
Omathanu
O
Children's Hospital of Michigan, Wayne State University, Detroit, MI 48201, USA.
Khandare
Jayant
J
Kolhe
Parag
P
Kannan
Sujatha
S
Lieh-Lai
Mary
M
Kannan
Rangaramanujam M
RM
D001692:Biological Transport; D045744:Cell Line, Tumor; D050091:Dendrimers / Q000737:chemistry*; D015232:Dinoprostone / Q000096:biosynthesis; D004337:Drug Carriers / Q000737:chemistry*; D006801:Humans; D008775:Methylprednisolone / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology; D008856:Microscopy, Fluorescence; D008970:Molecular Weight
D050091:Dendrimers; D004337:Drug Carriers; C104700:PAMAM Starburst; D015232:Dinoprostone; D008775:Methylprednisolone