Functional analysis of the stress-inducible soybean calmodulin isoform-4 (GmCaM-4) promoter in transgenic tobacco plants.
The transcription of soybean (Glycine max) calmodulin isoform-4 (GmCaM-4) is dramatically induced within 0.5 h of exposure to pathogen or NaCl. Core cis-acting elements that regulate the expression of the GmCaM-4 gene in response to pathogen and salt stress were previously identified, between -1,207 and -1,128 bp, and between -858 and -728 bp, in the GmCaM-4 promoter. Here, we characterized the properties of the DNA-binding complexes that form at the two core cis-acting elements of the GmCaM-4 promoter in pathogen-treated nuclear extracts. We generated GUS reporter constructs harboring various deletions of approximately 1.3-kb GmCaM-4 promoter, and analyzed GUS expression in tobacco plants transformed with these constructs. The GUS expression analysis suggested that the two previously identified core regions are involved in inducing GmCaM-4 expression in the heterologous system. Finally, a transient expression assay of Arabidopsis protoplasts showed that the GmCaM-4 promoter produced greater levels of GUS activity than did the CaMV35S promoter after pathogen or NaCl treatments, suggesting that the GmCaM-4 promoter may be useful in the production of conditional gene expression systems.
Park
Hyeong Cheol
HC
Division of Applied Life Science, Plant Molecular Biology and Biotechnology Research Center and Environmental Biotechnology National Core Research Center, Gyeongsang National University, Jinju, Korea. hcpark@gnu.ac.kr
Kim
Man Lyang
ML
Kang
Yun Hwan
YH
Jeong
Jae Cheol
JC
Cheong
Mi Sun
MS
Choi
Wonkyun
W
Lee
Sang Yeol
SY
Cho
Moo Je
MJ
Kim
Min Chul
MC
Chung
Woo Sik
WS
Yun
Dae-Jin
DJ
D001665:Binding Sites; D002147:Calmodulin / Q000096:biosynthesis / Q000235:genetics*; D004268:DNA-Binding Proteins; D018506:Gene Expression Regulation, Plant; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics; D030821:Plants, Genetically Modified; D011401:Promoter Regions, Genetic; D020033:Protein Isoforms; D017422:Sequence Analysis, DNA; D013025:Glycine max / Q000235:genetics* / Q000378:metabolism; D013312:Stress, Physiological; D014026:Nicotiana / Q000235:genetics* / Q000378:metabolism; D015533:Transcriptional Activation
D002147:Calmodulin; D004268:DNA-Binding Proteins; D010940:Plant Proteins; D020033:Protein Isoforms