Norwalk virus-like particles bind specifically to A, H and difucosylated Lewis but not to B histo-blood group active glycosphingolipids.
Noroviruses and norovirus virus-like particles (VLPs) exhibit strain specific patterns in their binding to ABH and Lewis histo-blood group antigens. In this study we demonstrate for the first time specific binding of Norwalk virus VLPs to type 1 and type 2 chain glycosphingolipids (GSLs) carrying ABH and Lewis antigens. N-succinimidyl-3-tributylstannyl benzoate (ATE) was precursor labeled with (125)I and then conjugated to VLPs. The (125)I-VLPs were used in GSL thin-layer chromatogram binding assays and displayed binding to H type 1, Lewis b, A type 1, A Lewis b GSLs but no binding to B type 1 or B Lewis b GSLs. For the type 2 chain GSLs the Norwalk VLPs bound to H type 2, Lewis y, A type 2 and A Lewis y. In addition, the VLPs bound to several complex GSLs from blood group O and A, but not from blood group B red blood cells.
Nilsson
Jonas
J
Department of Clinical Chemistry and Transfusion Medicine, Institute of Biomedicine, University of Gothenburg, Sahlgrenska University Hospital, Gothenburg, SE-413 45, Sweden. jonas.nilsson@clinchem.gu.se
Rydell
Gustaf E
GE
Le Pendu
Jacques
J
Larson
Göran
G
D000017:ABO Blood-Group System / Q000378:metabolism*; D002240:Carbohydrate Sequence; D002851:Chromatography, High Pressure Liquid; D004912:Erythrocytes / Q000378:metabolism; D005643:Fucose / Q000378:metabolism*; D006028:Glycosphingolipids / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D006801:Humans; D007457:Iodine Radioisotopes; D007983:Lewis Blood Group Antigens / Q000378:metabolism*; D008969:Molecular Sequence Data; D009663:Norwalk virus / Q000378:metabolism*; D014771:Virion / Q000378:metabolism*
D000017:ABO Blood-Group System; D006028:Glycosphingolipids; D007457:Iodine Radioisotopes; D007983:Lewis Blood Group Antigens; D005643:Fucose