title: [Effect of allyl glycoside extracted from Herba Rhodiolae on activity and proliferation of cultured Schwann cells under high glucose condition].
abstract: OBJECTIVE
  To observe changes in activity and proliferation of cultured Schwann cells under the high glucose condition and effect of allyl glycoside extracted from Herba Rhodiolae (AG) on these changes for exploring the possible mechanism of diabetic peripheral nerve injury and the pharmacologic action of AG on them.
  
  
  METHODS
  Adopting refined Brokes method, Schwann cells were isolated from the sciatic nerve tissue of newborn Wistar rats and purified. Their activity and proliferative capability were determined using XTT method and 3H-TdR incorporative method respectively.
  
  
  RESULTS
  High glucose showed marked inhibitory effect on both activity and proliferative capability of Schwann cells, and the inhibition could be markedly improved by AG.
  
  
  CONCLUSIONS
  Diabetic peripheral nerve injury is possibly related to inhibitory effect of high glucose on activity and proliferative capability of Schwann cells. Effect of AG in improving these inhibitory changes provides the experiment basis for clinical use of traditional Chinese medicine, Herba Rhodiolae.
authors.affiliation: Department of Neurology, Huashan Hospital Affiliated to Fudan University, Shanghai.
authors.forename: Bo-Shui
authors.initials: BS
authors.lastname: Wu
authors.forename: Wen-Jian
authors.initials: WJ
authors.lastname: Wang
authors.forename: Wei-Hua
authors.initials: WH
authors.lastname: Chen
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D003929:Diabetic Neuropathies / Q000209:etiology; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005947:Glucose / Q000494:pharmacology*; D006027:Glycosides / Q000302:isolation & purification / Q000494:pharmacology*; D008297:Male; D010946:Plants, Medicinal / Q000737:chemistry*; D051381:Rats; D017208:Rats, Wistar; D012583:Schwann Cells / Q000166:cytology*; D012584:Sciatic Nerve / Q000166:cytology
chemical_list: D004365:Drugs, Chinese Herbal; D006027:Glycosides; D005947:Glucose