[Recombinant adenovirus-mediated expression of 1.2-copy hepatitis B virus DNA in three hepatocytes].
OBJECTIVE
To study hepatitis B virus (HBV) expression in 3 hepatocytes infected with recombinant adenovirus containing 1.2-copy HBV DNA.a


METHODS
A chicken hepatoma cell line and two human hepatocytes were infected by the recombinant adenovirus containing 1.2-copy HBV DNA at 25 pfu/cell. HBV-specific mRNA was detected by RT-PCR 3 days after the infection, and HBsAg and HBeAg were detected by ELISA and HBV DNA by real-time PCR daily after the infection.


RESULTS
HBV mRNA expression was detected in all the 3 cells after recombinant adenovirus infection, and the quantities of HBV DNA and HBV antigens in the culture supernatant increased with the passage of time.a


CONCLUSIONS
Infection with the recombinant adenovirus containing 1.2-copy HBV DNA can mediate HBV infection in the 3 cells in vitro.
Liang
Wei-fang
WF
Department of Infectious Diseases, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China. weifl@fimmu.com
Liu
Zhi-hua
ZH
Luo
Kang-xian
KX
D000256:Adenoviridae / Q000235:genetics*; D000818:Animals; D045744:Cell Line, Tumor; D017077:Culture Media, Conditioned / Q000378:metabolism; D004274:DNA, Recombinant / Q000235:genetics*; D004279:DNA, Viral / Q000235:genetics* / Q000378:metabolism; D015870:Gene Expression; D006511:Hepatitis B Antigens / Q000378:metabolism; D006515:Hepatitis B virus; D022781:Hepatocytes / Q000378:metabolism* / Q000821:virology; D006801:Humans; D020133:Reverse Transcriptase Polymerase Chain Reaction
D017077:Culture Media, Conditioned; D004274:DNA, Recombinant; D004279:DNA, Viral; D006511:Hepatitis B Antigens