Volatile organic compounds: a potential direct  long-distance mechanism for antagonistic action  of Fusarium oxysporum strain MSA 35.
Fusarium oxysporum MSA35 [wild-type (WT) strain] is an antagonistic Fusarium that lives in association with a consortium of bacteria belonging to the genera Serratia, Achromobacter, Bacillus and Stenotrophomonas in an Italian soil suppressive to Fusarium wilt. Typing experiments and virulence tests provided evidence that the F. oxysporum isolate when cured of the bacterial symbionts [the cured (CU) form], is pathogenic, causing wilt symptoms identical to those caused by F. oxysporum f. sp. lactucae. Here, we demonstrate that small volatile organic compounds (VOCs) emitted from the WT strain negatively influence the mycelial growth of different formae speciales of F. oxysporum. Furthermore, these VOCs repress gene expression of two putative virulence genes in F. oxysporum lactucae strain Fuslat10, a fungus against which the WT strain MSA 35 has antagonistic activity. The VOC profile of the WT and CU fungus shows different compositions. Sesquiterpenes, mainly caryophyllene, were present in the headspace only of WT MSA 35. No sesquiterpenes were found in the volatiles of ectosymbiotic Serratia sp. strain DM1 and Achromobacter sp. strain MM1. Bacterial volatiles had no effects on the growth of the different ff. spp. of F. oxysporum examined. Hyphae grownwithVOCfrom WT F. oxysporum f. sp. lactucae strain MSA 35 were hydrophobic whereas those grown without VOCs were not, suggesting a correlation between the presence of volatiles in the atmosphere and the phenotype of the mycelium. This is the first report of VOC production by antagonistic F. oxysporum MSA35 and their effects on pathogenic F. oxysporum. The results obtained in this work led us to propose a new potential direct long-distance mechanism for antagonism by F. oxysporum MSA 35 mediated by VOCs. Antagonism could be the consequence of both reduction of pathogen mycelial growth and inhibition of pathogen virulence gene expression.
Minerdi
Daniela
D
Centre of Competence for Innovation in Agro-Environmental Field, Agroinnova, University of Torino, via Leonardo da Vinci, 44, 10095 Grugliasco, Italy. daniela.minerdi@unito.it
Bossi
Simone
S
Gullino
Maria Lodovica
ML
Garibaldi
Angelo
A
D000898:Antibiosis; D005670:Fusarium / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism* / Q000502:physiology*; D015966:Gene Expression Regulation, Fungal / Q000187:drug effects; D007558:Italy; D025282:Mycelium / Q000187:drug effects / Q000254:growth & development; D012988:Soil Microbiology; D037521:Virulence Factors / Q000096:biosynthesis; D055549:Volatile Organic Compounds / Q000378:metabolism*
D037521:Virulence Factors; D055549:Volatile Organic Compounds