title: RNA preparation and characterization for gene expression studies.
abstract: Much information can be obtained from knowledge of the relative expression level of each gene in the transcriptome. With the current advances in technology as little as a single cell is required as starting material for gene expression experiments. The mRNA from a single cell may be linearly amplified to an excess of 10(6)-fold. Reverse transcription and fluorescent labeling of the amplified RNA yields a stable target for subsequent hybridization to DNA microarrays.
authors.affiliation: University of Copenhagen, Copenhagen, Denmark.
authors.forename: Michael
authors.initials: M
authors.lastname: Stangegaard
mesh_terms: D020869:Gene Expression Profiling / Q000379:methods*; D006801:Humans; D016133:Polymerase Chain Reaction; D012313:RNA / Q000302:isolation & purification*; D048348:Reverse Transcription / Q000235:genetics; D013194:Staining and Labeling
chemical_list: D012313:RNA