title: Plasma amino acid responses after consumption of beverages with varying protein type.
abstract: OBJECTIVE
  To examine how different proteins in a carbohydrate-protein beverage affect postprandial amino acid (AA), glucose, and insulin responses.
  
  
  METHODS
  Two randomized, repeated-measures experiments were performed. In one, 10 volunteers drank 3 carbohydrate-protein beverages (380 kcal, 76 g carbohydrate, 19 g protein, 2 g fat) in separate (>7 days) trials, each differing in protein type. All drinks consisted of cocoa (4 g) and nonfat dry milk (1 g) supplemented with casein (CAS), whey (WP), or a casein and whey blend (CAS-WP). Ten additional volunteers consumed the same drinks after 60 min of varying-intensity exercise (60% and 85% VO2peak). Blood glucose, insulin, glucose-dependent insulinotrophic polypeptide (GIP), and AAs were measured every 15-30 min for 4 hr after beverage consumption.
  
  
  RESULTS
  Branched-chain AA concentrations peaked at 30 min and did not differ between beverages at rest (0.69 +/- 0.12 mmol/L) or postexercise (0.70 +/- 0.07 mmol/L). There were no significant differences between beverages with respect to initial (time 0-60) or total area under the curve (time 0-240) for any outcome measures at rest or postexercise.
  
  
  CONCLUSIONS
  High-carbohydrate beverages containing various proportions of milk proteins procured from a supplier to the commercial industry had no impact on AA concentration. Retrospective chemical analysis of commercial proteins showed that casein was partially hydrolyzed; therefore, consumers should carefully consider the manufacturer (to ensure that the product contains intact protein) or other factors (i.e., cost or taste) when procuring these beverages for their purported physiological effects.
authors.affiliation: Military Nutrition Division, U.S. Army Research Institute of Environmental Medicine, Natick, MA, USA.
authors.forename: Tracey J
authors.initials: TJ
authors.lastname: Smith
authors.forename: Scott J
authors.initials: SJ
authors.lastname: Montain
authors.forename: Danielle
authors.initials: D
authors.lastname: Anderson
authors.forename: Andrew J
authors.initials: AJ
authors.lastname: Young
mesh_terms: D000596:Amino Acids / Q000097:blood*; D000597:Amino Acids, Branched-Chain / Q000097:blood; D019540:Area Under Curve; D001628:Beverages; D001786:Blood Glucose / Q000378:metabolism; D018592:Cross-Over Studies; D004040:Dietary Carbohydrates / Q000008:administration & dosage / Q000378:metabolism; D004044:Dietary Proteins / Q000008:administration & dosage* / Q000378:metabolism; D004311:Double-Blind Method; D015444:Exercise / Q000502:physiology*; D005749:Gastric Inhibitory Polypeptide / Q000097:blood; D006801:Humans; D007328:Insulin / Q000097:blood; D008297:Male; D009124:Muscle Proteins / Q000378:metabolism*; D019518:Postprandial Period; D055815:Young Adult
chemical_list: D000596:Amino Acids; D000597:Amino Acids, Branched-Chain; D001786:Blood Glucose; D004040:Dietary Carbohydrates; D004044:Dietary Proteins; D007328:Insulin; D009124:Muscle Proteins; D005749:Gastric Inhibitory Polypeptide