title: A system for high-resolution depth-resolved optical imaging of fluorescence and absorption contrast.
abstract: Laminar optical tomography (LOT) is a new three-dimensional in vivo functional optical imaging technique. Adopting a microscopy-based setup and diffuse optical tomography (DOT) imaging principles, LOT can perform both absorption- and fluorescence-contrast imaging with higher resolution (100-200 microm) than DOT and deeper penetration (2-3 mm) than laser scanning microscopy. These features, as well as a large field of view and acquisition speeds up to 100 frames per second, make LOT suitable for depth-resolved imaging of stratified tissues such as retina, skin, endothelial tissues and the cortex of the brain. In this paper, we provide a detailed description of a new LOT system design capable of imaging both absorption and fluorescence contrast, and present characterization of its performance using phantom studies.
authors.affiliation: Department of Biomedical Engineering, Laboratory for Functional Optical Imaging, Columbia University, New York 10027, USA.
authors.forename: Baohong
authors.initials: B
authors.lastname: Yuan
authors.forename: Sean A
authors.initials: SA
authors.lastname: Burgess
authors.forename: Amir
authors.initials: A
authors.lastname: Iranmahboob
authors.forename: Matthew B
authors.initials: MB
authors.lastname: Bouchard
authors.forename: Nicole
authors.initials: N
authors.lastname: Lehrer
authors.forename: Clémence
authors.initials: C
authors.lastname: Bordier
authors.forename: Elizabeth M C
authors.initials: EM
authors.lastname: Hillman
mesh_terms: D000042:Absorption; D002138:Calibration; D004581:Electronics; D004867:Equipment Design; D005453:Fluorescence; D018613:Microscopy, Confocal; D019047:Phantoms, Imaging; D012680:Sensitivity and Specificity; D013997:Time Factors; D041622:Tomography, Optical / Q000295:instrumentation*