title: Spontaneous immortalization of oligodendroglial cells derived from an SV40 T antigen-positive human glioblastoma multiforme.
abstract: The polyoma group of viruses, including SV40, is known to be oncogenic in certain species. Here we report for the first time naturally occurring, immortalized tumor cells from a patient with glioblastoma multiforme (GBM); the cells were shown to be oligodendroglia; cells had developed remarkable chromosomal changes and were positive for SV40 T antigen. Therefore, we postulated that the main cause of immortalization of these cells was the expression of SV40 T antigen gene and protein. Since the cells are naturally generated, they will provide a useful model to study the function of oligodendroglial cells and the development of GBM.
authors.affiliation: Ilsong Institute of Life Science, Hallym University, Ilsong B/D 1605-4, Gwanyang-dong, Dongan-gu, Anyang, Gyeonggi-do 431-060, South Korea.
authors.forename: Boe-Hyun
authors.initials: BH
authors.lastname: Kim
authors.forename: Jun-Ho
authors.initials: JH
authors.lastname: Song
authors.forename: Yong-Chul
authors.initials: YC
authors.lastname: Jeon
authors.forename: Byung-Hoon
authors.initials: BH
authors.lastname: Jeong
authors.forename: Sook-Kyung
authors.initials: SK
authors.lastname: Yun
authors.forename: Hyoun-Chan
authors.initials: HC
authors.lastname: Cho
authors.forename: Richard I
authors.initials: RI
authors.lastname: Carp
authors.forename: Yong-Sun
authors.initials: YS
authors.lastname: Kim
mesh_terms: D000952:Antigens, Polyomavirus Transforming / Q000235:genetics; D015153:Blotting, Western; D001932:Brain Neoplasms / Q000235:genetics / Q000821:virology*; D002461:Cell Line, Transformed; D002472:Cell Transformation, Viral / Q000235:genetics; D002869:Chromosome Aberrations; D005260:Female; D005909:Glioblastoma / Q000235:genetics / Q000821:virology*; D006801:Humans; D007150:Immunohistochemistry; D008875:Middle Aged; D009836:Oligodendroglia / Q000166:cytology* / Q000821:virology; D034741:RNA, Small Interfering; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014162:Transfection
chemical_list: D000952:Antigens, Polyomavirus Transforming; D034741:RNA, Small Interfering