Development of a 1-step cell-based assay for cost-effective screening of antiviral drugs for vaccinia virus.
We report a 1-step assay to screen antiviral substances combining the simultaneous application of cells, virus sample, and drug to 96-well plates. The results were obtained within 26 h when vaccinia virus plaques were counted or virus-induced cytopathic effect was measured. This fast cost-effective procedure may be used for other cytopathic viruses and is suitable to 1st screening tests.
Jesus
Desyreé M
DM
Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Ilha do Fundão, Brazil.
Santos
Elida S
ES
Schnellrath
Laila C
LC
Damaso
Clarissa R
CR
D000818:Animals; D000998:Antiviral Agents / Q000302:isolation & purification*; D002460:Cell Line; D003362:Cost-Benefit Analysis; D003588:Cytopathogenic Effect, Viral; D004353:Drug Evaluation, Preclinical / Q000191:economics* / Q000379:methods*; D000882:Haplorhini; D006801:Humans; D014616:Vaccinia virus / Q000187:drug effects*; D010948:Viral Plaque Assay
D000998:Antiviral Agents