Stage- and tissue-specific expression of rice OsIsu1 gene encoding a scaffold protein for mitochondrial iron-sulfur-cluster biogenesis.
Isu is a scaffold protein involved in mitochondrial iron-sulfur-cluster biogenesis, which affects redox and iron homeostasis in human and yeast cells. A BLASTP search identified two putative Isu genes in rice, and we designated one of them as OsIsu1. When expressed in onion epidermal cells, OsIsu1::GFP was localized to the mitochondria. Northern analysis showed that OsIsu1 was down-regulated in iron-deficient rice root. OsIsu1 promoter-GUS was introduced into Arabidopsis thaliana and histochemical GUS-staining showed that OsIsu1 expression was regulated in a stage- and tissue-specific manner. OsIsu1 was expressed ectopically in Arabidopsis under the control of the CaMV35S promoter, which increased weight of plants.
Tsugama
Daisuke
D
Asian Natural Environmental Science Center, The University of Tokyo, 1-1-1 Midori-cho, Nishitokyo-shi, Tokyo, 188-0002, Japan.
Liu
Shenkui
S
Takano
Tetsuo
T
D017360:Arabidopsis / Q000737:chemistry / Q000235:genetics; D019677:Artificial Gene Fusion; D015152:Blotting, Northern; D002478:Cells, Cultured; D005786:Gene Expression Regulation; D017930:Genes, Reporter; D005966:Glucuronidase / Q000235:genetics / Q000378:metabolism; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D007506:Iron-Sulfur Proteins / Q000096:biosynthesis* / Q000235:genetics; D024101:Mitochondrial Proteins / Q000096:biosynthesis* / Q000235:genetics; D019697:Onions; D012275:Oryza / Q000737:chemistry / Q000502:physiology*; D018517:Plant Roots / Q000737:chemistry; D030821:Plants, Genetically Modified / Q000737:chemistry / Q000235:genetics
D007506:Iron-Sulfur Proteins; D024101:Mitochondrial Proteins; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins; D005966:Glucuronidase