The gastric ulcer-healing action of allylpyrocatechol is mediated by modulation of arginase metabolism and shift of cytokine balance.
The role of the ariginine-metabolism in the healing action of the Piper betle phenol, allylpyrocatechol (APC) and omeprazole against indomethacin-induced stomach ulceration in mouse was investigated. Indomethacin (18 mg/kg) was found to induce maximum stomach ulceration in Swiss albino mice on the 3rd day of its administration, which was associated with reduced arginase activity (21.6%, P<0.05), endothelial nitric oxide synthase (eNOS) expression (72%, P<0.001), and IL-4 and TGF-beta levels, along with increased inducible nitric oxide synthase (iNOS) (9.3 folds, P<0.001) expression, nitrite (2.29 folds, P<0.001), IL-1beta and IL-6 generation. Besides providing comparable healing as omeprazole (3 mg/kg x 3 days), APC (5 mg/kg x 3 days) shifted the iNOS/NO axis to the arginase/polyamine axis as revealed from the increased arginase activity (73.1%, P<0.001), eNOS expression (67.8%, P<0.001), and reduced iNOS expression (65.6%, P<0.001) and nitrite level (53.2%, P<0.001). These can be attributed to a favourable anti-/pro-inflammatory cytokines ratio, generated by APC. The healing by omeprazole was however, not significantly associated with those parameters.
Yadav
Sudhir K
SK
Department of Biochemistry, Dr. B.C. Roy Post Graduate Institute of Basic Medical Sciences, Kolkata, India.
Adhikary
Biplab
B
Maity
Biswanath
B
Bandyopadhyay
Sandip K
SK
Chattopadhyay
Subrata
S
D000818:Animals; D000893:Anti-Inflammatory Agents / Q000378:metabolism; D001119:Arginase / Q000378:metabolism*; D001120:Arginine / Q000378:metabolism; D002396:Catechols / Q000494:pharmacology* / Q000627:therapeutic use*; D016207:Cytokines / Q000097:blood / Q000378:metabolism*; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D008297:Male; D051379:Mice; D009092:Mucous Membrane / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism; D009569:Nitric Oxide / Q000096:biosynthesis; D019001:Nitric Oxide Synthase / Q000378:metabolism; D009573:Nitrites / Q000097:blood; D009195:Peroxidase / Q000378:metabolism; D013276:Stomach Ulcer / Q000097:blood / Q000188:drug therapy* / Q000201:enzymology / Q000378:metabolism
D000893:Anti-Inflammatory Agents; D002396:Catechols; D016207:Cytokines; D009573:Nitrites; C523074:allylpyrocatechol; D009569:Nitric Oxide; D001120:Arginine; D009195:Peroxidase; D019001:Nitric Oxide Synthase; D001119:Arginase