title: Ultraviolet light (254 nm) inactivation of Listeria monocytogenes on frankfurters that contain potassium lactate and sodium diacetate.
abstract: Listeria monocytogenes, a psychrotrophic foodborne pathogen, is an occasional postprocess contaminant on ready-to-eat meat (RTE) products including frankfurters. Ultraviolet C light (UVC) is an FDA-approved technology for the decontamination of food surfaces. In this study, the ability of UVC to inactivate L. monocytogenes on frankfurters that contained potassium lactate (PL) and sodium diacetate (SDA), either before or after packaging, was investigated. UVC irradiation of frankfurters that were surface-inoculated with L. monocytogenes resulted in a 1.31, 1.49, and 1.93 log reduction at doses of 1, 2, and 4 J/cm(2), respectively. UVC treatment had no effect on frankfurter color or texture at UVC doses up to 4 J/cm(2). Frankfurter meat treated with UVC doses up to 16 J/cm(2) did not increase mutagenesis in bacterial or human cells, either with or without exogenous metabolic activation. UVC treatment of single-layer frankfurter packs at a dose of 2 J/cm(2) resulted in a 0.97 (+/- 0.14) log reduction of L. monocytogenes. Following 8 wk of refrigerated storage L. monocytogenes levels decreased by only 0.65 log in non-UVC-treated frankfurter packs compared with 2.5 log in the UVC-treated packs. Because the numbers of L. monocytogenes associated with contaminations of ready-to-eat meats are typically very low, the use of UVC in combination with potassium lactate and sodium diacetate has the potential to reduce the number of frankfurter recalls and foodborne illness outbreaks.
authors.affiliation: Eastern Regional Research Center, Agricultural Research Service, U.S. Dept. of Agriculture, 600 East Mermaid Lane, Wyndmoor, PA 19038, USA.
authors.forename: C H
authors.initials: CH
authors.lastname: Sommers
authors.forename: P H
authors.initials: PH
authors.lastname: Cooke
authors.forename: X
authors.initials: X
authors.lastname: Fan
authors.forename: J E
authors.initials: JE
authors.lastname: Sites
mesh_terms: D000818:Animals; D003080:Cold Temperature; D015169:Colony Count, Microbial; D005516:Food Microbiology; D018857:Food Packaging; D005519:Food Preservation; D007773:Lactates / Q000032:analysis*; D008089:Listeria monocytogenes / Q000528:radiation effects*; D008461:Meat Products / Q000032:analysis / Q000382:microbiology* / Q000528:radiation effects; D018613:Microscopy, Confocal; D008855:Microscopy, Electron, Scanning; D019346:Sodium Acetate / Q000032:analysis*; D013997:Time Factors; D014466:Ultraviolet Rays
chemical_list: D007773:Lactates; D019346:Sodium Acetate