title: Ebolavirus VP35 interacts with the cytoplasmic dynein light chain 8.
abstract: The viral protein VP35 of ebolavirus (EBOV) is implicated to have diverse roles in the viral life cycle. We employed a yeast two-hybrid screen to search for VP35 binding partners and identified the cytoplasmic dynein light chain (DLC8) as a protein that interacts with VP35. Mapping analysis unraveled a consensus motif, SQTQT, within VP35 through which VP35 binds to DLC8. The disruption of DLC8 binding does not affect the ability of VP35 to inhibit type I IFN production. Given that VP35 from various EBOV species interacts with DLC8, this interaction may have a role in regulating the EBOV life cycle.
authors.affiliation: Department of Virology III, National Institute of Infectious Diseases, Gakuen 4-7-1, Musashi-Murayama, Tokyo 208-0011, Japan. kubota@nih.go.jp
authors.forename: Toru
authors.initials: T
authors.lastname: Kubota
authors.forename: Mayumi
authors.initials: M
authors.lastname: Matsuoka
authors.forename: Tsung-Hsien
authors.initials: TH
authors.lastname: Chang
authors.forename: Mike
authors.initials: M
authors.lastname: Bray
authors.forename: Steven
authors.initials: S
authors.lastname: Jones
authors.forename: Masato
authors.initials: M
authors.lastname: Tashiro
authors.forename: Atsushi
authors.initials: A
authors.lastname: Kato
authors.forename: Keiko
authors.initials: K
authors.lastname: Ozato
mesh_terms: D020816:Amino Acid Motifs; D001665:Binding Sites; D002460:Cell Line; D056445:Cytoplasmic Dyneins; D004398:Dyneins / Q000378:metabolism*; D029043:Ebolavirus / Q000737:chemistry*; D006801:Humans; D019590:Nucleocapsid Proteins; D009698:Nucleoproteins / Q000378:metabolism*; D011485:Protein Binding; D025941:Protein Interaction Mapping; D014758:Viral Core Proteins / Q000378:metabolism*
chemical_list: D019590:Nucleocapsid Proteins; D009698:Nucleoproteins; D014758:Viral Core Proteins; C084818:nucleoprotein VP35, Ebola virus; C427758:DYNLL1 protein, human; D056445:Cytoplasmic Dyneins; D004398:Dyneins