title: Biomolecular-motor-based nano- or microscale particle translocations on DNA microarrays.
abstract: We aimed to create autonomous on-chip systems that perform targeted translocations of nano- or microscale particles in parallel using machinery that mimics biological systems. By exploiting biomolecular-motor-based motility and DNA hybridization, we demonstrate that single-stranded DNA-labeled microtubules gliding on kinesin-coated surfaces acted as cargo translocators and that single-stranded DNA-labeled cargoes were loaded/unloaded onto/from gliding microtubules at micropatterned loading/unloading sites specified by DNA base sequences. Our results will help to create autonomous molecular sorters and sensors.
authors.affiliation: Department of Life Sciences, Graduate School of Arts and Sciences, The University of Tokyo, Tokyo 153-8902, Japan. hiyama@nttdocomo.co.jp
authors.forename: Satoshi
authors.initials: S
authors.lastname: Hiyama
authors.forename: Riho
authors.initials: R
authors.lastname: Gojo
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Shima
authors.forename: Shoji
authors.initials: S
authors.lastname: Takeuchi
authors.forename: Kazuo
authors.initials: K
authors.lastname: Sutoh
mesh_terms: D000818:Animals; D001483:Base Sequence; D004277:DNA, Single-Stranded / Q000737:chemistry*; D016547:Kinesins / Q000737:chemistry*; D008870:Microtubules / Q000737:chemistry*; D018716:Molecular Mimicry; D053758:Nanoparticles / Q000737:chemistry*; D020411:Oligonucleotide Array Sequence Analysis; D010316:Particle Size; D013552:Swine
chemical_list: D004277:DNA, Single-Stranded; D016547:Kinesins