Nanoholes as nanochannels: flow-through plasmonic sensing.
We combine nanofluidics and nanoplasmonics for surface-plasmon resonance (SPR) sensing using flow-through nanohole arrays. The role of surface plasmons on resonant transmission motivates the application of nanohole arrays as surface-based biosensors. Research to date, however, has focused on dead-ended holes, and therefore failed to harness the benefits of nanoconfined transport combined with SPR sensing. The flow-through format enables rapid transport of reactants to the active surface inside the nanoholes, with potential for significantly improved time of analysis and biomarker yield through nanohole sieving. We apply the flow-through method to monitor the formation of a monolayer and the immobilization of an ovarian cancer biomarker specific antibody on the sensing surface in real-time. The flow-through method resulted in a 6-fold improvement in response time as compared to the established flow-over method.
Eftekhari
Fatemeh
F
Electrical and Computer Engineering, University of Victoria, Victoria, British Columbia, Canada.
Escobedo
Carlos
C
Ferreira
Jacqueline
J
Duan
Xiaobo
X
Girotto
Emerson M
EM
Brolo
Alexandre G
AG
Gordon
Reuven
R
Sinton
David
D
D000906:Antibodies / Q000032:analysis* / Q000276:immunology; D014408:Biomarkers, Tumor / Q000032:analysis / Q000276:immunology; D004867:Equipment Design; D005260:Female; D006801:Humans; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D049329:Nanostructures / Q000737:chemistry*; D036103:Nanotechnology / Q000295:instrumentation*; D010051:Ovarian Neoplasms / Q000175:diagnosis / Q000276:immunology; D020349:Surface Plasmon Resonance / Q000191:economics / Q000295:instrumentation* / Q000379:methods*; D013997:Time Factors
D000906:Antibodies; D014408:Biomarkers, Tumor