title: Chemiluminescent PEG-PCL micelles for imaging hydrogen peroxide.
abstract: Hydrogen peroxide is one of the fundamental molecules of biology, regulating key cell signaling pathways and the development of numerous inflammatory diseases. There is therefore great interest in developing contrast agents that can detect hydrogen peroxide in vitro and in vivo. In this report, we present a new contrast agent for imaging hydrogen peroxide, termed the chemiluminescent poly(ethylene glycol)-b-poly(e-caprolactone) (PEGPCL) micelles (CPMs), which can detect hydrogen peroxide at nanomolar concentrations and chemiluminesce in the near IR range (676 nm) in the presence of hydrogen peroxide. The CPMs are composed of a PEG-PCL scaffold and have fluorescent dyes and peroxalate esters in their hydrophobic PCL core. The CPMs image hydrogen peroxide by undergoing a three-component chemiluminescent reaction involving a peroxalate ester, a fluorescent dye, and hydrogen peroxide. The CPMs also have a stealth PEG corona to enhance their circulation half life. The CPMs should find numerous applications for imaging hydrogen peroxide because of their nanomolar sensitivity, small size, and stealth pegylated surface.
authors.affiliation: The Wallace H. Coulter Department of Biomedical Engineering and Parker H. Petit Institute of Bioengineering and Bioscience, Georgia Institute of Technology, Atlanta, 30332, USA.
authors.forename: Madhuri
authors.initials: M
authors.lastname: Dasari
authors.forename: Dongwon
authors.initials: D
authors.lastname: Lee
authors.forename: Venkata Reddy
authors.initials: VR
authors.lastname: Erigala
authors.forename: Niren
authors.initials: N
authors.lastname: Murthy
mesh_terms: D002021:Buffers; D002208:Caproates / Q000138:chemical synthesis / Q000737:chemistry*; D006861:Hydrogen Peroxide / Q000097:blood / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D021621:Imaging, Three-Dimensional; D007783:Lactones / Q000138:chemical synthesis / Q000737:chemistry*; D049449:Luminescence; D008823:Micelles; D009279:Naphthacenes / Q000737:chemistry; D011092:Polyethylene Glycols / Q000138:chemical synthesis / Q000737:chemistry*; D012542:Scattering, Radiation; D014867:Water
chemical_list: D002021:Buffers; D002208:Caproates; D007783:Lactones; D008823:Micelles; D009279:Naphthacenes; D014867:Water; D011092:Polyethylene Glycols; C045049:rubrene; C121056:caprolactone; D006861:Hydrogen Peroxide