Rapid identification of improved protein ligands using peptoid microarrays.
A rapid array-based protocol is presented by which a modest affinity protein-binding small molecule can be appended to a library of peptoids via click chemistry. The array can then be screened for improved ligands that exhibit a higher affinity for the protein target.
Lim
Hyun-Suk
HS
Department of Internal Medicine, Division of Translational Research, University of Texas Southwestern Medical Center, Dallas, TX 75390-9185, USA.
Reddy
M Muralidhar
MM
Xiao
Xiangshu
X
Wilson
Johnnie
J
Wilson
Rosemary
R
Connell
Steven
S
Kodadek
Thomas
T
D008024:Ligands; D034444:Peptoids / Q000138:chemical synthesis / Q000737:chemistry*; D040081:Protein Array Analysis; D011506:Proteins / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism
D008024:Ligands; D034444:Peptoids; D011506:Proteins