title: Patch testing of patients allergic to Disperse Blue 106 and Disperse Blue 124 with thin-layer chromatograms and purified dyes.
abstract: BACKGROUND
  Former investigations have demonstrated that patch test preparations containing Disperse Blue (DB) 106 and DB 124 are impure.
  
  
  OBJECTIVE
  To investigate the significance of impurities in preparations of disperse dyes with regard to contact allergy.
  
  
  METHODS
  21 patients allergic to DB 106 and/or DB 124 were tested with dilution series of commercial and purified DB 106 and DB 124. All patients were additionally tested with thin-layer chromatography (TLC) strips made from the commercial preparations containing DB 106 and 18 of them also with TLC strips containing DB 124.
  
  
  RESULTS
  13 of 21 and 12/18 patients tested positively to the strips of DB 106 and DB 124, respectively. Among these patients, 4/13 and 5/12, respectively, did not react to the main spot but reacted to other spots. Dilution series of purified DB 106 and DB 124 were positive in 10 patients each, while 16 and 15 patients, respectively, tested positively to the dilution series of the corresponding commercial disperse dye.
  
  
  CONCLUSIONS
  Approximately 25% of the patients, diagnosed as contact allergic to DB 106 and DB 124, only reacted to impurities in the patch test preparations. This has implications for diagnosis and prevention.
authors.affiliation: Department of Occupational and Environmental Dermatology, Lund University, Malmö University Hospital, Malmö, Sweden. kristina.morgardt-ryberg@med.lu.se
authors.forename: Kristina
authors.initials: K
authors.lastname: Ryberg
authors.forename: An
authors.initials: A
authors.lastname: Goossens
authors.forename: Marléne
authors.initials: M
authors.lastname: Isaksson
authors.forename: Birgitta
authors.initials: B
authors.lastname: Gruvberger
authors.forename: Erik
authors.initials: E
authors.lastname: Zimerson
authors.forename: Lena
authors.initials: L
authors.lastname: Persson
authors.forename: Magnus
authors.initials: M
authors.lastname: Bruze
mesh_terms: D000328:Adult; D000368:Aged; D001391:Azo Compounds / Q000633:toxicity*; D002855:Chromatography, Thin Layer; D017449:Dermatitis, Allergic Contact / Q000175:diagnosis* / Q000209:etiology*; D004305:Dose-Response Relationship, Drug; D004340:Drug Contamination; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D010328:Patch Tests / Q000379:methods* / Q000592:standards; D012015:Reference Standards; D013548:Sweden
chemical_list: D001391:Azo Compounds; C069319:Disperse Blue 124; C049175:disperse blue 106