Serological and molecular detection of Borrelia burgdorferi sensu lato and Anaplasmataceae in rodents.
The presence of Anaplasma spp. and Borrelia burgdorferi sensu lato in rodents from Eastern Slovakia were followed by serological and molecular methods. The seroprevalence for Borrelia was detected in 16.6 %, for Anaplasmataceae (APT) in 13.2 % and co-occurrence of Borrelia and APT in 7.5 %. Out of 110 ear biopsies of rodents, 5 were B. afzelii-positive. Five biopsies tested positive with the Ehr521-Ehr747 primers amplifying all the members of the family APT. A. phagocytophilum was detected in 1.8 %, 2.7 % were infected with Anaplasma-like organisms. Co-occurrence of Borrelia and Anaplasma in ear biopsies was found in 1.8 %. The circulation of both Borrelia and Anaplasma in the region of Eastern Slovakia was confirmed.
Stefancíková
A
A
Department of Natural Foci Diseases, Parasitological Institute, Slovak Academy of Sciences, Kosice, Slovakia. astefan@saske.sk
Derdáková
M
M
Lencáková
D
D
Ivanová
R
R
Stanko
M
M
Cisláková
L
L
Petko
B
B
D000710:Anaplasmataceae / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D000711:Anaplasmataceae Infections / Q000175:diagnosis / Q000453:epidemiology / Q000382:microbiology / Q000662:veterinary*; D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D003411:Arvicolinae / Q000382:microbiology*; D015748:Borrelia burgdorferi Group / Q000302:isolation & purification*; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification; D004197:Disease Reservoirs; D004431:Ear, External / Q000382:microbiology; D004797:Enzyme-Linked Immunosorbent Assay; D008193:Lyme Disease / Q000175:diagnosis / Q000453:epidemiology / Q000382:microbiology / Q000662:veterinary*; D051379:Mice; D008969:Molecular Sequence Data; D051189:Murinae / Q000382:microbiology*; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D015203:Reproducibility of Results; D012376:Rodent Diseases / Q000175:diagnosis / Q000453:epidemiology / Q000382:microbiology*; D016415:Sequence Alignment; D016036:Seroepidemiologic Studies; D018154:Slovakia / Q000453:epidemiology
D000907:Antibodies, Bacterial; D004269:DNA, Bacterial