The effect of ultra-nanocrystalline diamond films on the proliferation and differentiation of neural stem cells.
The interaction of ultra-nanocrystalline diamond (UNCD) with neural stem cells (NSCs) has been studied along with its surface modification in order to improve its function as a biomaterial. Hydrogen- and oxygen-terminated UNCD films were compared with standard grade polystyrene in terms of their impact on the growth, expansion and differentiation of NSCs. When NSCs were cultured on these substrates in low serum and without any differentiating factors, hydrogen-terminated UNCD films spontaneously induced cell proliferation and neuronal differentiation. Oxygen-terminated UNCD films were also shown to further improve neural differentiation, with a preference to differentiate into oligodendrocytes. Hence, controlling the surface properties of UNCD could manipulate the differentiation of NSCs for different biomedical applications. These observations raise the potential for the use of UNCD as a biomaterial for central nervous system transplantation and tissue engineering.
Chen
Ying-Chieh
YC
Department of Materials Science and Engineering, National Tsing-Hua University, Hsin-Chu, Taiwan.
Lee
Don-Ching
DC
Hsiao
Chao-Yang
CY
Chung
Yu-Fen
YF
Chen
Huang-Chin
HC
Thomas
Joseph P
JP
Pong
Way-Faung
WF
Tai
Nyan-Hwa
NH
Lin
I-Nan
IN
Chiu
Ing-Ming
IM
D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry / Q000378:metabolism; D018929:Cell Culture Techniques / Q000295:instrumentation*; D002454:Cell Differentiation / Q000502:physiology*; D049109:Cell Proliferation; D048430:Cell Shape; D002478:Cells, Cultured; D018130:Diamond / Q000737:chemistry*; D006801:Humans; D008422:Materials Testing; D051379:Mice; D009474:Neurons / Q000166:cytology / Q000502:physiology*; D011137:Polystyrenes / Q000737:chemistry; D013234:Stem Cells / Q000166:cytology / Q000502:physiology*; D013499:Surface Properties; D014404:Tubulin / Q000378:metabolism
D001672:Biocompatible Materials; D011137:Polystyrenes; D014404:Tubulin; C462941:beta3 tubulin, mouse; D018130:Diamond