Fluorinated carbohydrates as lectin ligands: versatile sensors in 19F-detected saturation transfer difference NMR spectroscopy.
As a novel approach for studying carbohydrate-lectin interactions spectroscopically, we combine the resolution and specificity of (19)F-detected NMR spectroscopy with the sensitivity of the saturation transfer difference (STD) technique. The resulting background-free (19)F-STD spectra open a promising perspective for broad application with medical relevance, for example, in drug discovery.
Diercks
Tammo
T
NMR facility, CiC bioGUNE, Parque Tecnológico de Bizkaia, Ed. 800, 48160 Derio, Spain.
Ribeiro
João P
JP
Cañada
F Javier
FJ
André
Sabine
S
Jiménez-Barbero
Jesús
J
Gabius
Hans-Joachim
HJ
D000465:Algorithms; D002241:Carbohydrates / Q000737:chemistry*; D015195:Drug Design; D055808:Drug Discovery; D005461:Fluorine / Q000737:chemistry*; D006001:Glycoconjugates / Q000737:chemistry; D037102:Lectins / Q000737:chemistry* / Q000378:metabolism; D008024:Ligands; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods*
D002241:Carbohydrates; D006001:Glycoconjugates; D037102:Lectins; D008024:Ligands; D005461:Fluorine