Effect of HO-1 cDNA-liposome complex transfer on erectile signalling of aged rats.
This work aimed to assess the efficacy of haeme oxygenase-1 (HO-1) cDNA-liposome complex transfer as a mediator of erectile signalling in aged rats. One hundred and fifty aged white albino rats were equally divided into five groups: controls, rats receiving lipofectamine, rats receiving intracorporeal HO-1 cDNA-lipsome complex, rats receiving HO-1 cDNA-liposome complex plus nitric oxide synthase (NOS) inhibitor, and rats receiving HO-1 cDNA-liposome complex plus HO inhibitor. Six rats were killed from each group after 12, 24 and 48 h, and after1 and 2 weeks. In dissected cavernous tissues, the following were assessed: HO-1 gene expression, Western blot for HO-1, HO enzyme activity, cGMP and histopathology. The results showed that HO-1 cDNA-liposome complex transfer led to a significant increase in cavernous tissue HO-1 protein, HO-1 gene expression, HO enzyme activity and cGMP up to 1 week. NOS inhibition exhibited no effect on HO-1 gene enhancement of cavernous tissue HO enzyme activity or cGMP, whereas inhibition of HO significantly decreased these parameters. Histopathology of cavernous tissue demonstrated a significant dilatation of helicine arteries in HO-1 cDNA-liposome complex treated group after 48 h compared with the controls. It is concluded that HO-1 cDNA-liposome complex transfer augments cavernous tissue cGMP with subsequent sinusoidal relaxation.
Abdel Aziz
M T
MT
Department of Medical Biochemistry, Faculty of Medicine, Cairo University, Cairo, Egypt.
Mostafa
T
T
Atta
H
H
Mahfouz
S
S
Wassef
M
M
Fouad
H
H
Kamel
M
M
Rashed
L
L
Sabry
D
D
Mouhamed
O
O
D000375:Aging; D000818:Animals; D002248:Carbon Monoxide / Q000494:pharmacology; D018076:DNA, Complementary / Q000627:therapeutic use; D004789:Enzyme Activation / Q000187:drug effects; D007172:Erectile Dysfunction / Q000628:therapy*; D015870:Gene Expression; D018014:Gene Transfer Techniques; D006162:Guanylate Cyclase / Q000378:metabolism; D051547:Heme Oxygenase-1 / Q000096:biosynthesis / Q000627:therapeutic use*; D008081:Liposomes / Q000627:therapeutic use*; D008297:Male; D019331:NG-Nitroarginine Methyl Ester / Q000627:therapeutic use; D010410:Penile Erection / Q000235:genetics / Q000502:physiology*; D051381:Rats; D018160:Receptors, Cytoplasmic and Nuclear / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D000071756:Soluble Guanylyl Cyclase
D018076:DNA, Complementary; D008081:Liposomes; D018160:Receptors, Cytoplasmic and Nuclear; D002248:Carbon Monoxide; D051547:Heme Oxygenase-1; D006162:Guanylate Cyclase; D000071756:Soluble Guanylyl Cyclase; D019331:NG-Nitroarginine Methyl Ester