title: Specificity of the second messenger pathways involved in basic fibroblast growth factor-induced survival and neurite growth in chick ciliary ganglion neurons.
abstract: Basic fibroblast growth factor (bFGF) exerts multiple neurotrophic actions on cultured neurons from the ciliary ganglion of chick embryo, among them promotion of neuronal survival and of neurite outgrowth. To understand the specificity of the signal transduction cascades involved in the control of these processes, we used pharmacological inhibitors of the three main effectors known to act downstream of the bFGF receptor (FGFR): phospholipase Cgamma (PLCgamma), mitogen-activated protein kinase (MAPK), and phosphatidylinositol 3-kinase (PI3-K). Neuronal survival was assessed at 24 and 48 hr; neurite growth was analyzed both on dissociated neurons and on explants of whole ganglia. Our data show that only the PI3-K pathway is involved in the survival-promoting effect of bFGF; on the other hand, all three effectors converge on the enhancement of neurite outgrowth, both on isolated neurons and in whole ganglia.
authors.affiliation: Department of Animal and Human Biology, University of Torino, Torino, Italy.
authors.forename: Alessandra
authors.initials: A
authors.lastname: Gilardino
authors.forename: Silvia
authors.initials: S
authors.lastname: Farcito
authors.forename: Pollyanna
authors.initials: P
authors.lastname: Zamburlin
authors.forename: Chiara
authors.initials: C
authors.lastname: Audisio
authors.forename: Davide
authors.initials: D
authors.lastname: Lovisolo
mesh_terms: D000818:Animals; D002470:Cell Survival / Q000187:drug effects / Q000502:physiology; D002478:Cells, Cultured / Q000166:cytology / Q000187:drug effects; D002642:Chick Embryo; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology / Q000502:physiology*; D005726:Ganglia, Parasympathetic / Q000166:cytology / Q000187:drug effects*; D020935:MAP Kinase Signaling System / Q000187:drug effects / Q000502:physiology; D009419:Nerve Tissue Proteins / Q000187:drug effects / Q000502:physiology; D016501:Neurites / Q000187:drug effects* / Q000502:physiology; D009924:Organ Culture Techniques; D019869:Phosphatidylinositol 3-Kinases / Q000502:physiology; D051966:Phospholipase C gamma / Q000502:physiology; D010766:Phosphorylation / Q000187:drug effects; D011499:Protein Processing, Post-Translational / Q000187:drug effects; D051496:Receptor, Fibroblast Growth Factor, Type 1 / Q000187:drug effects / Q000502:physiology; D015290:Second Messenger Systems / Q000187:drug effects* / Q000502:physiology
chemical_list: D009419:Nerve Tissue Proteins; D016222:Fibroblast Growth Factor 2; D019869:Phosphatidylinositol 3-Kinases; D051496:Receptor, Fibroblast Growth Factor, Type 1; D051966:Phospholipase C gamma