title: Characteristics of ethylnitrosourea-induced cataracts.
abstract: OBJECTIVE
  This study analyzed genes associated with the morphology and regulation of ethylnitrosourea (ENU)-induced cataract mouse.
  
  
  METHODS
  Immunohistochemistry analysis using anti-crystallins and PCNA antibody revealed that the localization pattern of these specific markers differed between the cataractous and wild-type lens epithelium. Two-dimensional electrophoresis and microarray techniques were used to identify the proteins and genes related to ENU-induced cataract.
  
  
  RESULTS
  A novel ENU-induced mutation in the mouse led to nuclear and cortical opacity of the eye lens at 5 weeks postnatal. This cataract phenotype was similar to that of the zonular-pulverulent type of human cataract. Crystallin proteins and gap-junction genes have relations to the formation of cataract.
  
  
  CONCLUSIONS
  Together, the results suggest that various proteins affect the formation and specific phenotypes of ENU-induced cataract mouse.
authors.affiliation: Department of Oral Biology, Research Center for Orofacial Hard Tissue Regeneration, Brain Korea 21 Project, Oral Science Research Center, College of Dentistry, Yonsei Center of Biotechnology, Yonsei University, Seoul, Korea.
authors.forename: Min-Jung
authors.initials: MJ
authors.lastname: Lee
authors.forename: Jae-Young
authors.initials: JY
authors.lastname: Kim
authors.forename: Yeun-Jung
authors.initials: YJ
authors.lastname: Kim
authors.forename: Jae-Woo
authors.initials: JW
authors.lastname: Cho
authors.forename: Kyu-Hyuk
authors.initials: KH
authors.lastname: Cho
authors.forename: Chang-Woo
authors.initials: CW
authors.lastname: Song
authors.forename: Han-Sung
authors.initials: HS
authors.lastname: Jung
mesh_terms: D000818:Animals; D002386:Cataract / Q000139:chemically induced* / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D017630:Connexins / Q000235:genetics; D003459:Crystallins / Q000235:genetics / Q000378:metabolism; D004252:DNA Mutational Analysis; D015180:Electrophoresis, Gel, Two-Dimensional; D005038:Ethylnitrosourea; D005136:Eye Proteins / Q000235:genetics; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D010641:Phenotype; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism; D040081:Protein Array Analysis; D014018:Tissue Distribution
chemical_list: D017630:Connexins; D003459:Crystallins; D005136:Eye Proteins; D018809:Proliferating Cell Nuclear Antigen; D005038:Ethylnitrosourea