title: Syndecan-2 overexpression regulates adhesion and migration through cooperation with integrin alpha2.
abstract: Syndecan-2, a transmembrane heparan sulfate proteoglycan, is known to serve as an adhesion receptor, but details of the regulatory mechanism governing syndecan-2 cell adhesion and migration remain unclear. Here, we examined this regulatory mechanism, showing that overexpression of syndecan-2 enhanced collagen adhesion, cell migration and invasion of normal rat intestinal epithelial cells (RIE1), and increased integrin alpha2 expression levels. Interestingly, RIE1 cells transfected with either syndecan-2 or integrin alpha2 showed similar adhesion and migration patterns, and a function-blocking anti-integrin alpha2 antibody abolished syndecan-2-mediated adhesion and migration. Consistent with these findings, transfection of integrin alpha2 siRNA diminished syndecan-2-induced cell migration in HCT116 human colon cancer cells. Taken together, these results demonstrate a novel cooperation between syndecan-2 and integrin alpha2beta1 in adhesion-mediated cell migration and invasion. This interactive dynamic might be a possible mechanism underlying the tumorigenic activities of colon cancer cells.
authors.affiliation: Department of Life Sciences, Division of Life and Pharmaceutical Sciences and the Center for Cell Signaling & Drug Discovery Research, Ewha Womans University, Seoul 120-750, Republic of Korea.
authors.forename: Sojoong
authors.initials: S
authors.lastname: Choi
authors.forename: Yeonhee
authors.initials: Y
authors.lastname: Kim
authors.forename: Haein
authors.initials: H
authors.lastname: Park
authors.forename: Inn-Oc
authors.initials: IO
authors.lastname: Han
authors.forename: Eunkyung
authors.initials: E
authors.lastname: Chung
authors.forename: Sung-Yul
authors.initials: SY
authors.lastname: Lee
authors.forename: Yong-Bae
authors.initials: YB
authors.lastname: Kim
authors.forename: Jung Weon
authors.initials: JW
authors.lastname: Lee
authors.forename: Eok-Soo
authors.initials: ES
authors.lastname: Oh
authors.forename: Jae Youn
authors.initials: JY
authors.lastname: Yi
mesh_terms: D000818:Animals; D002448:Cell Adhesion; D045744:Cell Line, Tumor; D002465:Cell Movement; D003094:Collagen / Q000737:chemistry / Q000378:metabolism; D003110:Colonic Neoplasms / Q000378:metabolism / Q000473:pathology; D020895:Enterocytes / Q000378:metabolism / Q000502:physiology*; D006801:Humans; D039421:Integrin alpha2 / Q000235:genetics / Q000378:metabolism*; D009361:Neoplasm Invasiveness; D034741:RNA, Small Interfering / Q000235:genetics; D051381:Rats; D053669:Syndecan-2 / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D039421:Integrin alpha2; D034741:RNA, Small Interfering; C508247:Sdc2 protein, rat; D053669:Syndecan-2; D003094:Collagen