title: Overexpression of methionine-R-sulfoxide reductases has no influence on fruit fly aging.
abstract: Methionine sulfoxide reductases (Msrs) are enzymes that repair oxidized methionine residues in proteins. This function implicated Msrs in antioxidant defense and the regulation of aging. There are two known Msr types in animals: MsrA specific for the reduction of methionine-S-sulfoxide, and MsrB that catalyzes the reduction of methionine-R-sulfoxide. In a previous study, overexpression of MsrA in the nervous system of Drosophila was found to extend lifespan by 70%. Overexpression of MsrA in yeast also extended lifespan, whereas MsrB overexpression did so only under calorie restriction conditions. The effect of MsrB overexpression on lifespan has not yet been characterized in animal model systems. Here, the GAL4-UAS binary system was used to drive overexpression of cytosolic Drosophila MsrB and mitochondrial mouse MsrB2 in whole body, fatbody, and the nervous system of flies. In contrast to MsrA, MsrB overexpression had no consistent effect on the lifespan of fruit flies on either corn meal or sugar yeast diets. Physical activity, fecundity, and stress resistance were also similar in MsrB-overexpressing and control flies. Thus, MsrA and MsrB, the two proteins with similar function in antioxidant protein repair, have different effects on aging in fruit flies.
authors.affiliation: Department of Biochemistry and Redox Biology Center, University of Nebraska, Lincoln, NE 68588, USA.
authors.forename: Valentina A
authors.initials: VA
authors.lastname: Shchedrina
authors.forename: Gerd
authors.initials: G
authors.lastname: Vorbrüggen
authors.forename: Byung Cheon
authors.initials: BC
authors.lastname: Lee
authors.forename: Hwa-Young
authors.initials: HY
authors.lastname: Kim
authors.forename: Hadise
authors.initials: H
authors.lastname: Kabil
authors.forename: Lawrence G
authors.initials: LG
authors.lastname: Harshman
authors.forename: Vadim N
authors.initials: VN
authors.lastname: Gladyshev
mesh_terms: D000375:Aging / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D030801:Animals, Genetically Modified; D029721:Drosophila Proteins / Q000096:biosynthesis / Q000235:genetics; D004331:Drosophila melanogaster; D015971:Gene Expression Regulation, Enzymologic; D051150:Methionine Sulfoxide Reductases / Q000096:biosynthesis / Q000235:genetics; D051379:Mice; D008840:Microfilament Proteins; D010088:Oxidoreductases / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D029721:Drosophila Proteins; D008840:Microfilament Proteins; D010088:Oxidoreductases; D051150:Methionine Sulfoxide Reductases; C520510:Msrb2 protein, mouse; C027219:methionine sulfoxide reductase; C495234:SelR protein, Drosophila