Surface plasmon resonance imaging of polymer microarrays to study protein-polymer interactions in high throughput.
Polymer microarrays provide a high-throughput format in which to assess biointerfacial interactions. This endeavor greatly assists with the development of advanced biomaterials. In order to increase the scope of this platform technology, the development of analytical tools that are compatible with the microarray format and are capable of analyzing biomolecular interactions in high throughput is needed. Here, we show that surface plasmon resonance imaging (SPRi) is such a tool. SPRi enables spatially resolved, surface sensitive, label free, real-time analysis of multiple surface-biomolecular interactions in parallel. In order to demonstrate this, we first printed phenylazide-modified polymers onto a slide coated with a low fouling base polymer. UV irradiation of the slide resulted in the cross-linking of the printed polymer spots to the surface. SPRi was then employed to study the adsorption and desorption of bovine serum albumin, collagen, and fibronectin to these adhesive microarray spots. The spots were also incubated with an adherent cell line, enabling insight into the underlying mechanisms of cell attachment to the polymers studied. For the system analyzed here, electrostatic interactions were shown to dominate cell attachment.
Hook
Andrew L
AL
School of Chemistry, Physics and Earth Sciences, Flinders University, Adelaide, 5001, South Australia, Australia.
Thissen
Helmut
H
Voelcker
Nicolas H
NH
D000818:Animals; D002417:Cattle; D045744:Cell Line, Tumor; D046210:Microfluidic Analytical Techniques; D008856:Microscopy, Fluorescence; D011108:Polymers / Q000737:chemistry*; D011506:Proteins / Q000737:chemistry*; D020349:Surface Plasmon Resonance / Q000379:methods
D011108:Polymers; D011506:Proteins