Cellular and molecular studies of the initial process of the photodynamic therapy in HEp-2 cells using LED light source and two different photosensitizers.
Photodynamic therapy is an attractive therapeutic procedure for a variety of neoplastic and non-neoplastic disorders that involve a tumor localizing photosensitizer that induces cytotoxicity when activated by a particular wavelength of light. Considering the acceptability of the phthalocyanine photosensitizers, we began to address initial cellular and molecular aspects involved in the therapy that leads to apoptosis or necrosis. After HEp-2 cells had been incubated for 1h with aluminium tetrasulfonate chloride (AlPcS(4)) or zinc phthalocyanines (ZnPc) at 10microM, they were irradiated with an LED prototype (640nm, 70mW, 4.5J/cm(2)) and subsequently analyzed at 0 and 3h after the irradiation through MTT, fluorescence microscopy, Annexin V-PI staining and gene transcription analysis. Based on the results we can address that the two photosensitizers led to 2 different death pathways; for AlPcS(4), the results suggest an initial process of intrinsic death pathway; and for ZnPc, a prostanoid-mediated activation of death machinery is suggested.
Machado
Aline Helena Araujo
AH
Laboratório de Biologia Celular & Tecidual, UNIVAP, São José dos Campos, SP, Brazil.
Pacheco Soares
Cristina
C
da Silva
Newton Soares
NS
Moraes
Karen C M
KC
D014408:Biomarkers, Tumor / Q000235:genetics / Q000378:metabolism; D016923:Cell Death; D006801:Humans; D007211:Indoles / Q000494:pharmacology / Q000633:toxicity*; D054833:Isoindoles; D007822:Laryngeal Neoplasms / Q000188:drug therapy / Q000473:pathology; D008027:Light; D010778:Photochemotherapy; D017319:Photosensitizing Agents / Q000494:pharmacology / Q000633:toxicity*; D013997:Time Factors; D014158:Transcription, Genetic; D014407:Tumor Cells, Cultured
D014408:Biomarkers, Tumor; D007211:Indoles; D054833:Isoindoles; D017319:Photosensitizing Agents; C028418:tetrasulfophthalocyanine; C013647:phthalocyanine