title: [The application of three methods combined for extracting DNA from human epithelium].
abstract: OBJECTIVE
  To improve DNA extraction from human epithelium.
  
  
  METHODS
  Chelex-100, Chelex-100 organic combined and Chelex-100 magnetism pearl combined methods were adopted according to the situation of each case.
  
  
  RESULTS
  With the stepwise extracting protocol, the trace amount of DNA was analyzed most efficiently and the success rate of DNA extracting was better improved.
  
  
  CONCLUSIONS
  The efficiency of DNA extraction from human epithelium could be greatly improved by modifying the extracting protocol such as purification and concentration based on previous results.
authors.affiliation: Forensic Science Institute of Public Security Ministry, Beijing 100038, China.
authors.forename: Yan-xia
authors.initials: YX
authors.lastname: Guo
authors.forename: Song
authors.initials: S
authors.lastname: Chen
authors.forename: Kai-hui
authors.initials: KH
authors.lastname: Liu
mesh_terms: D004247:DNA / Q000032:analysis / Q000302:isolation & purification*; D004847:Epithelial Cells / Q000166:cytology*; D053803:Forensic Genetics; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D012117:Resins, Synthetic / Q000737:chemistry
chemical_list: D012117:Resins, Synthetic; C024997:Chelex 100; D004247:DNA