Generation and utilization of anti-drug monoclonal antibodies for screening of 36 drug users by dot-ELISA.
In this study, we prepared monoclonal antibodies against morphine, methadone, babital, methamphetamine, and phencyclidine, then developed a dot-ELISA method by such antibodies to test their efficacy in clinical application and the screening of urine samples. It was found that there were 36 narcotics-positive drug users, including 28 morphine positive, six methamphetamine positive, and two positive for both. All the results were confirmed by commercial drug testing kits.
Wang
Shuang
S
Department of Biology, School of Applied Science, University of Science and Technology, Beijing, China.
Wei
Yuzhi
Y
Chen
Guangyu
G
Liu
Xiaowei
X
Jin
Haiming
H
Yan
Zhaoqi
Z
Wu
Qiaowen
Q
Du
Hongwu
H
D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000652:urine; D001462:Barbital / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006801:Humans; D008691:Methadone / Q000276:immunology*; D008694:Methamphetamine / Q000276:immunology*; D009020:Morphine / Q000276:immunology*; D010622:Phencyclidine / Q000276:immunology*; D015813:Substance Abuse Detection / Q000379:methods*
D000911:Antibodies, Monoclonal; D008694:Methamphetamine; D001462:Barbital; D009020:Morphine; D010622:Phencyclidine; D008691:Methadone