title: Tryptophan synthase: a mine for enzymologists.
abstract: Tryptophan synthase is a pyridoxal 5'-phosphate-dependent alpha(2)beta(2) complex catalyzing the last two steps of tryptophan biosynthesis in bacteria, plants and fungi. Structural, dynamic and functional studies, carried out over more than 40 years, have unveiled that: (1) alpha- and beta-active sites are separated by about 20 A and communicate via the selective stabilization of distinct conformational states, triggered by the chemical nature of individual catalytic intermediates and by allosteric ligands; (2) indole, formed at alpha-active site, is intramolecularly channeled to the beta-active site; and (3) naturally occurring as well as genetically generated mutants have allowed to pinpoint functional and regulatory roles for several individual amino acids. These key features have made tryptophan synthase a text-book case for the understanding of the interplay between chemistry and conformational energy landscapes.
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Parma, Viale G P Usberti 23/A, Parma, Italy.
authors.forename: Samanta
authors.initials: S
authors.lastname: Raboni
authors.forename: Stefano
authors.initials: S
authors.lastname: Bettati
authors.forename: Andrea
authors.initials: A
authors.lastname: Mozzarelli
mesh_terms: D000494:Allosteric Regulation; D001665:Binding Sites; D055162:Biocatalysis; D007211:Indoles / Q000737:chemistry; D007700:Kinetics; D008024:Ligands; D008956:Models, Chemical; D008958:Models, Molecular; D011485:Protein Binding; D011487:Protein Conformation; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D011732:Pyridoxal Phosphate / Q000378:metabolism; D014367:Tryptophan Synthase / Q000737:chemistry* / Q000378:metabolism*
chemical_list: D007211:Indoles; D008024:Ligands; D011732:Pyridoxal Phosphate; C030374:indole; D014367:Tryptophan Synthase