title: Transfection microarray and the applications.
abstract: Microarray transfection has been extensively studied for high-throughput functional analysis of mammalian cells. However, control of efficiency and reproducibility are the critical issues for practical use. By using solid-phase transfection accelerators and nano-scaffold, we provide a highly efficient and reproducible microarray-transfection device, "transfection microarray". The device would be applied to the limited number of available primary cells and stem cells not only for large-scale functional analysis but also reporter-based time-lapse cellular event analysis.
authors.affiliation: National Institute of Advanced Industrial Science and Technology, 2-42 Aomi, Koto-ku, Tokyo, 135-0064, Japan. masato-miyake@aist.go.jp
authors.forename: Masato
authors.initials: M
authors.lastname: Miyake
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Yoshikawa
authors.forename: Satoshi
authors.initials: S
authors.lastname: Fujita
authors.forename: Jun
authors.initials: J
authors.lastname: Miyake
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D006801:Humans; D008954:Models, Biological; D049329:Nanostructures / Q000737:chemistry; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D014162:Transfection / Q000379:methods*