Extracellular matrix gene expression during wound healing of the injured rat vocal fold.
OBJECTIVE
To measure the expression of procollagen-I and -III, decorin, and hyaluronan synthase (HAS)-1, -2, and -3 during the inflammatory, proliferative, and remodeling phases of rat vocal fold injury.


METHODS
Prospective, animal model.


METHODS
Vocal folds were injured in 30 rats. Injured specimens were harvested on postinjury days 1, 3, 7, 14, 28, and 56. Five uninjured rats were used for polymerase chain reaction (PCR) control. Real-time PCR was used to measure the expression of procollagen-I and -III, decorin, and hyaluronan synthase (HAS)-1, -2, and -3.


RESULTS
Compared with control, expression of procollagen-I and -III were significantly decreased on postinjury days 1 and 56; decorin expression was significantly decreased on postinjury days 1, 3, 7, and 56; HAS-1 expression was significantly decreased on postinjury days 3, 7, 14, 28, and 56; HAS-2 expression was significantly decreased on postinjury days 28 and 56; HAS-3 expression was significantly decreased on postinjury day 56.


CONCLUSIONS
Results revealed time-dependent alterations in the expression of genes coding procollagen-I and -III, decorin, and HAS-1, -2, and -3. Knowledge of the temporal regulation of these genes and underlying histology will be used in future studies to investigate molecular approaches for manipulation of vocal fold injury.
Ohno
Tsunehisa
T
Department of Otolaryngology, Vanderbilt University Bill Wilkerson Center for Otolaryngology and Communication Sciences, Nashville, TN, USA.
Hirano
Shigeru
S
Rousseau
Bernard
B
D000704:Analysis of Variance; D000818:Animals; D058575:Decorin; D005109:Extracellular Matrix / Q000235:genetics*; D016326:Extracellular Matrix Proteins / Q000235:genetics*; D015870:Gene Expression; D014453:Glucuronosyltransferase / Q000235:genetics*; D000076002:Hyaluronan Synthases; D008297:Male; D011347:Procollagen / Q000235:genetics*; D011446:Prospective Studies; D011509:Proteoglycans / Q000235:genetics*; D051381:Rats; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014827:Vocal Cords / Q000293:injuries*; D014945:Wound Healing / Q000235:genetics*
C546880:Dcn protein, rat; D058575:Decorin; D016326:Extracellular Matrix Proteins; D011347:Procollagen; D011509:Proteoglycans; D014453:Glucuronosyltransferase; D000076002:Hyaluronan Synthases