Route to smooth silica-based surfaces decorated with novel self-assembled monolayers (SAMs) containing glycidyl-terminated very long hydrocarbon chains.
Novel glycidyl-terminated organosilicon coupling agents possessing a trialkoxysilyl head group and a very long hydrocarbon chain (C22) were synthesized. Their ability to afford densely packed self-assembled monolayers (SAMs) grafted on silica-based surfaces was investigated. Transmission FT-IR spectra showed that the most regular films were obtained by using trichloracetic acid as the catalyst (10 M%). Atomic force microscopy (AFM) and optical ellipsometry were consistent with well ordered monolayers exhibiting a marked decrease of the surface roughness. Epifluorescence microscopy revealed that these SAMs possessed a better surface reactivity than monolayers obtained with the commercially available (3-glycidoxypropyl) trimethoxysilane (GPTS) upon grafting of a fluorescent probe (dansylcadaverin). Moreover, direct attachment of fluorescent antibodies (RAG-TRITC) through covalent binding led to higher mean fluorescence intensities, showing that these new SAMs possess high potential for the immobilization of biological molecules.
Dinh
Duy Hai
DH
Université de Bordeaux, Institut des Sciences Moléculaires, UMR 5255-CNRS, 351 cours de la Libération, 33405 Talence Cedex, France.
Vellutini
Luc
L
Bennetau
Bernard
B
Dejous
Corinne
C
Rebière
Dominique
D
Pascal
Emilie
E
Moynet
Daniel
D
Belin
Colette
C
Desbat
Bernard
B
Labrugère
Christine
C
Pillot
Jean-Paul
JP
D002103:Cadaverine / Q000031:analogs & derivatives / Q000737:chemistry; D002384:Catalysis; D003432:Cross-Linking Reagents / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry; D008567:Membranes, Artificial; D018625:Microscopy, Atomic Force; D012821:Silanes / Q000737:chemistry*; D012822:Silicon Dioxide / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods; D013499:Surface Properties
C403136:3-glycidoxypropyltrimethoxysilane; D003432:Cross-Linking Reagents; D005456:Fluorescent Dyes; D008567:Membranes, Artificial; D012821:Silanes; D012822:Silicon Dioxide; C008542:monodansylcadaverine; D002103:Cadaverine