title: Long-term maintenance of fungal cultures on perlite in cryovials - an alternative for agar slants.
abstract: Cultures of 33 basidiomycete strains out of 35 tested were viable with unchanged characteristics after four years of maintenance on perlite in cryovials. These cultures can be a good substitute for agar cultures in long-term maintenance of fungi. For comparison, the storage under oil was evaluated but it turned out to be unsuitable for the majority of our cultures.
authors.affiliation: Institute of Microbiology, Academy of Sciences of the Czech Republic, Prague, Czech Republic. homolka@biomed.cas.cz
authors.forename: L
authors.initials: L
authors.lastname: Homolka
authors.forename: L
authors.initials: L
authors.lastname: Lisá
mesh_terms: D000537:Aluminum Oxide; D001487:Basidiomycota / Q000201:enzymology / Q000254:growth & development*; D003080:Cold Temperature; D003470:Culture Media; D005656:Fungal Proteins / Q000378:metabolism; D042845:Laccase / Q000378:metabolism; D009172:Mycology / Q000295:instrumentation / Q000379:methods*; D009821:Oils; D011309:Preservation, Biological / Q000295:instrumentation / Q000379:methods*; D012822:Silicon Dioxide; D013997:Time Factors
chemical_list: D003470:Culture Media; D005656:Fungal Proteins; D009821:Oils; C003076:Perlite; D012822:Silicon Dioxide; D042845:Laccase; D000537:Aluminum Oxide