title: Detection of canine parvovirus type 2c by a commercially available in-house rapid test.
abstract: Diagnosis of canine parvovirus (CPV) infection is usually carried out by means of rapid immunochromatographic assays, but the ability of these tests to detect all CPV variants, including the recently identified CPV-2c, is still debated. To determine if the assays detect the different CPV variants, 201 CPV PCR-positive faecal samples or rectal swabs were tested using a commercially available in-house test. Specimens (CPV-2a, n=51; CPV-2b, n=50; CPV-2c, n=100), containing CPV DNA loads >10(5) DNA copies/mg faeces, as determined by real-time PCR, were selected from previous studies. The percentage of positive in-house tests was 80.4%, 78.0% and 77.0% for CPV types 2a, 2b and 2c, respectively, confirming the ability of the test to detect the new variant CPV-2c. However, considering the sensitivity limits of the in-house tests that have been observed previously, negative results from the in-house test kit should be confirmed by PCR-based methods.
authors.affiliation: Department of Veterinary Public Health, Faculty of Veterinary Medicine, Strada per Casamassima km 3, 70010 Valenzano (Bari), Italy. n.decaro@veterinaria.uniba.it
authors.forename: Nicola
authors.initials: N
authors.lastname: Decaro
authors.forename: Costantina
authors.initials: C
authors.lastname: Desario
authors.forename: Melissa J
authors.initials: MJ
authors.lastname: Beall
authors.forename: Alessandra
authors.initials: A
authors.lastname: Cavalli
authors.forename: Marco
authors.initials: M
authors.lastname: Campolo
authors.forename: Anthony A
authors.initials: AA
authors.lastname: Dimarco
authors.forename: Francesca
authors.initials: F
authors.lastname: Amorisco
authors.forename: Maria Loredana
authors.initials: ML
authors.lastname: Colaianni
authors.forename: Canio
authors.initials: C
authors.lastname: Buonavoglia
mesh_terms: D000818:Animals; D004279:DNA, Viral / Q000032:analysis; D004283:Dog Diseases / Q000175:diagnosis* / Q000821:virology; D004285:Dogs; D005243:Feces / Q000821:virology; D010322:Parvoviridae Infections / Q000175:diagnosis / Q000662:veterinary* / Q000821:virology; D017993:Parvovirus, Canine / Q000145:classification / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary*; D012680:Sensitivity and Specificity; D013997:Time Factors
chemical_list: D004279:DNA, Viral