Intrinsic innervation of the horse ileum.
This paper describes the morphology and distribution of the enteric nervous system (ENS) cells and fibres immunoreactive for choline acetyltransferase (ChAT), neuronal nitric oxide synthase (nNOS), substance P (SP), calcitonin gene-related peptide (CGRP), NF200kDa (NF200), and S100 protein. The percentages of subclasses of enteric neurons in the total neuronal population were investigated by the use of anti-PGP 9.5 or anti-NSE antibodies. ChAT-IR myenteric plexus (MP) and submucosal plexus (SMP) neurons were 66+/-7% and 74+/-15%, respectively, whereas those cells expressing nNOS-IR were 38+/-7% and 5+/-1%, respectively. MP and SMP neurons expressing both phenotypes were also present. SP-IR was expressed by 14+/-13% of MP and 66+/-8% of SMP neurons whereas CGRP-IR was observed only in the SMP (43+/-6%). NF200-IR was expressed by 61+/-15% and 91+/-10% of the MP and SMP neurons, respectively. The majority of the CGRP-IR SMP neurons expressed also SP-IR. Almost all SP-IR neurons in both the plexuses were cholinergic. The present study quantifies the main neuronal subpopulations of the ENS of the horse ileum; these data might be utilized to understand the neuronal modifications which occur in several gastrointestinal tract disorders.
Chiocchetti
Roberto
R
Department of Veterinary Morphophysiology and Animal Productions, University of Bologna, 40064 Ozzano Emilia, Bologna, Italy. roberto.chiocchetti@unibo.it
Bombardi
Cristiano
C
Mongardi-Fantaguzzi
Caterina
C
Venturelli
Elena
E
Russo
Domenico
D
Spadari
Alessandro
A
Montoneri
Corrado
C
Romagnoli
Noemi
N
Grandis
Annamaria
A
D000818:Animals; D000918:Antibody Specificity; D017615:Enteric Nervous System / Q000166:cytology / Q000502:physiology*; D005260:Female; D006736:Horses; D007082:Ileum / Q000294:innervation*; D007150:Immunohistochemistry; D007413:Intestinal Mucosa / Q000294:innervation; D008856:Microscopy, Fluorescence; D009197:Myenteric Plexus / Q000166:cytology / Q000502:physiology; D009474:Neurons / Q000166:cytology / Q000201:enzymology / Q000502:physiology; D052248:Nitric Oxide Synthase Type I / Q000032:analysis
D052248:Nitric Oxide Synthase Type I