Reactive nitrogen intermediate susceptibility of Mycobacterium tuberculosis genotypes in an urban setting.
BACKGROUND
Mycobacterium tuberculosis genotypes resistant to reactive nitrogen intermediates (RNI) predominate in certain urban communities, suggesting that this phenotype influences disease transmission.


OBJECTIVE
To compare different M. tuberculosis genotypes for resistance to RNI generated in vitro.


METHODS
We genotyped 420 M. tuberculosis isolates from a neighborhood in Sao Paulo, Brazil, and analyzed them for susceptibility to RNI generated in acidified sodium nitrite (ASN) solution.


RESULTS
Seventy-one (43%) of 167 recent-infection strains and 68 (43%) of 158 endogenous infection strains showed moderate- to high-level ASN resistance.


CONCLUSIONS
ASN resistance of M. tuberculosis is not necessarily a determining factor for enhanced transmission.
Inumaru
V T G
VT
Faculdade de Saúde Pública, University of Sao Paulo, Sao Paulo, Brazil.
Nogueira
P A
PA
Butuem
I V
IV
Riley
L W
LW
Ferrazoli
L
L
D001938:Brazil / Q000453:epidemiology; D004269:DNA, Bacterial / Q000235:genetics*; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D009169:Mycobacterium tuberculosis / Q000187:drug effects / Q000235:genetics*; D012150:Polymorphism, Restriction Fragment Length; D015995:Prevalence; D026361:Reactive Nitrogen Species / Q000494:pharmacology*; D012189:Retrospective Studies; D014376:Tuberculosis / Q000453:epidemiology / Q000235:genetics* / Q000382:microbiology; D014505:Urban Population
D004269:DNA, Bacterial; D026361:Reactive Nitrogen Species