title: Intracellular uptake of a new boronated porphyrin EC032.
abstract: We measured the toxicity and intracellular uptake of a newly developed boronated porphyrin EC032, and verified the fluorescence-based boron concentration measuring methods. Toxicity study showed that concentration required to produce a 50% reduction in viability (IC(50)) of EC032 was more than 0.25 mM. Fluorescence study showed the intracellular uptake of EC032 increased up until 24 h after its exposure to C6, 9L, U87, and U251 cells. There was also a linear correlation between ICP-AES and fluorescence intensity as an arbitrary unit about measurement of boron concentration. Fluorescence-based boron concentration measuring methods are very simple and useful methods, especially for screening of slight test dose of porphyrin compounds.
authors.affiliation: Department of Neurosurgery, Institute of Clinical Medicine, University of Tsukuba, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Tsurubuchi
authors.forename: T
authors.initials: T
authors.lastname: Yamamoto
authors.forename: K
authors.initials: K
authors.lastname: Nakai
authors.forename: A
authors.initials: A
authors.lastname: Zaboronok
authors.forename: F
authors.initials: F
authors.lastname: Yoshida
authors.forename: M
authors.initials: M
authors.lastname: Miyakawa
authors.forename: M
authors.initials: M
authors.lastname: Shirakawa
authors.forename: Y
authors.initials: Y
authors.lastname: Yamamoto
authors.forename: M
authors.initials: M
authors.lastname: Matsuda
authors.forename: A
authors.initials: A
authors.lastname: Matsumura
mesh_terms: D000818:Animals; D001693:Biological Transport, Active; D001896:Boron Compounds / Q000737:chemistry / Q000493:pharmacokinetics* / Q000627:therapeutic use / Q000633:toxicity; D016754:Boron Neutron Capture Therapy / Q000379:methods*; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000528:radiation effects; D006801:Humans; D015394:Molecular Structure; D011166:Porphyrins / Q000737:chemistry / Q000493:pharmacokinetics / Q000627:therapeutic use / Q000633:toxicity; D011838:Radiation-Sensitizing Agents / Q000737:chemistry / Q000493:pharmacokinetics* / Q000627:therapeutic use / Q000633:toxicity; D051381:Rats
chemical_list: D001896:Boron Compounds; D011166:Porphyrins; D011838:Radiation-Sensitizing Agents