title: [Application and development of spectroscopy methodologies in the study on non-covalent interactions].
abstract: Spectrophotometric method is widely used in the structure determination of biologic macromolecules and non-covalent interactions study for its convenience and speed. In the present paper, spectroscopy methodologies in the study of non-covalent interactions between small-molecule and biomacromolecule is comprehensively reviewed with 25 references. This review article focuses on the applications and development of common spectroscopy methodologies in the study of non-covalent interactions between small molecule and biomacromolecule,including the UV, fluorescence, CD, IR, Raman, resonance light scattering technique and SPR. The advantages and disadvantages of spectroscopy methodologies are also described. UV-Vis absorption spectrum (UV) method is widely used in the study of non-covalent interactions for its convenience and speed. The binding site number, the apparent binding constant and the interaction mode of non-covalent interactions can be obtained by fluorescence spectrum method. Circular dichroism (CD) method is effective way in the study of non-covalent interactions measure. Spectroscopy information about protein secondary structure and conformation can be acquired by infrared spectrometry (IR) method. Raman spectroscopy method is a better way to investigate the conformation change in macromolecules in solution. Non-covalent interactions can be measured by surface plasma resonance (SPR) method under the natural active condition. X-ray diffraction analysis method is better for non-covalent interactions research, but it is difficult to cultivate crystalline complex.
authors.affiliation: Henan Institute of Chemistry, Henan Academy of Sciences, Zhengzhou 450002, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Li
authors.forename: Ben-Cai
authors.initials: BC
authors.lastname: Dai
authors.forename: Yong-De
authors.initials: YD
authors.lastname: Zhao
authors.forename: Kui
authors.initials: K
authors.lastname: Lu