title: ESCRT ubiquitin-binding domains function cooperatively during MVB cargo sorting.
abstract: Ubiquitin (Ub) sorting receptors facilitate the targeting of ubiquitinated membrane proteins into multivesicular bodies (MVBs). Ub-binding domains (UBDs) have been described in several endosomal sorting complexes required for transport (ESCRT). Using available structural information, we have investigated the role of the multiple UBDs within ESCRTs during MVB cargo selection. We found a novel UBD within ESCRT-I and show that it contributes to MVB sorting in concert with the known UBDs within the ESCRT complexes. These experiments reveal an unexpected level of coordination among the ESCRT UBDs, suggesting that they collectively recognize a diverse set of cargo rather than act sequentially at discrete steps.
authors.affiliation: Department of Molecular Physiology and Biophysics, University of Iowa, Iowa City, IA 52240, USA.
authors.forename: S Brookhart
authors.initials: SB
authors.lastname: Shields
authors.forename: Andrea J
authors.initials: AJ
authors.lastname: Oestreich
authors.forename: Stanley
authors.initials: S
authors.lastname: Winistorfer
authors.forename: Doris
authors.initials: D
authors.lastname: Nguyen
authors.forename: Johanna A
authors.initials: JA
authors.lastname: Payne
authors.forename: David J
authors.initials: DJ
authors.lastname: Katzmann
authors.forename: Robert
authors.initials: R
authors.lastname: Piper
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002268:Carboxypeptidases / Q000235:genetics / Q000378:metabolism; D056827:Endosomal Sorting Complexes Required for Transport; D011992:Endosomes / Q000378:metabolism*; D008565:Membrane Proteins / Q000378:metabolism*; D008958:Models, Molecular; D008969:Molecular Sequence Data; D046912:Multiprotein Complexes / Q000378:metabolism*; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D021381:Protein Transport / Q000502:physiology; D051964:Receptors, Mating Factor / Q000235:genetics / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism; D025801:Ubiquitin / Q000378:metabolism*; D033921:Vesicular Transport Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D056827:Endosomal Sorting Complexes Required for Transport; D008565:Membrane Proteins; D046912:Multiprotein Complexes; C517100:Mvb12 protein, S cerevisiae; D051964:Receptors, Mating Factor; D011993:Recombinant Fusion Proteins; C081863:STE3 protein, S cerevisiae; C425333:STP22 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D025801:Ubiquitin; D033921:Vesicular Transport Proteins; C491128:Vps36 protein, S cerevisiae; D002268:Carboxypeptidases; C019836:CPS1 protein, S cerevisiae