title: [Induced differentiation of rat adipose-derived stem cells into Schwann-like cells].
abstract: OBJECTIVE
  To assess the differentiation potential of rat adipose-derived stem cells (ADSCs) into Schwann-like cells in vitro.
  
  
  METHODS
  ADSCs isolated from adult SD rats were cultured in vitro and identified with the cell surface antigens CD44, CD49d and CD106 by immunocytochemistry. The ADSCs of the sixth to eighth passages were inoculated in polylysine-coated culture plate and cultured for 12 days in DMEM/F12 culture medium containing 10% fetal bovine serum, 5 ng/ml platelet-derived growth factor, 10 ng/ml basic fibroblast growth factor, 14 micromol/L Forskolin and 200 ng/ml Heregulin to induce their differentiation in vitro. Immunocytochemistry was performed to identify the expression of the cell surface markers nestin, glial fibrillary acidic protein (GFAP), S-100, and P75.
  
  
  RESULTS
  The isolated and purified ADSCs were positive for CD44 and CD49d expressions but negative for CD106. After 12 days of culture in the conditional culture medium, most of the cells showed positive expressions of GFAP, S-100, and P75, the specific protein markers of Schwann cells.
  
  
  CONCLUSIONS
  Adult rat ADSCs are confirmed to have potentials of neuroglial differentiation and capable of differentiating into Schwann-like cells in vitro.
authors.affiliation: Department of Embryology, School of Basic Medical Sciences. Southern Medical University, Guangzhou 510515, China.
authors.forename: Yin-juan
authors.initials: YJ
authors.lastname: Tang
authors.forename: Li-hua
authors.initials: LH
authors.lastname: Zhang
authors.forename: Jie-ming
authors.initials: JM
authors.lastname: Liu
authors.forename: Wei-ren
authors.initials: WR
authors.lastname: Dong
authors.forename: Jia-song
authors.initials: JS
authors.lastname: Guo
authors.forename: Hai-hong
authors.initials: HH
authors.lastname: Wang
authors.forename: Xiang
authors.initials: X
authors.lastname: Dai
authors.forename: Ying-hua
authors.initials: YH
authors.lastname: Chen
authors.forename: Ying-qing
authors.initials: YQ
authors.lastname: Xiao
mesh_terms: D000273:Adipose Tissue / Q000166:cytology*; D000818:Animals; D002417:Cattle; D002454:Cell Differentiation; D049109:Cell Proliferation; D003584:Cytological Techniques / Q000379:methods*; D005786:Gene Expression Regulation; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D012583:Schwann Cells / Q000166:cytology* / Q000378:metabolism; D013234:Stem Cells / Q000166:cytology*