Metabolic labeling of sialic acids in living animals with alkynyl sugars.
Sialome sweet sialome: As sialic acids are involved in many host-pathogen recognition events and are markers of embryonic and malignant tissues, there is great interest in methods for the enrichment and identification of sialylated glycoproteins from complex tissues. Now N-(4-pentynoyl)mannosamine can be used to metabolically label sialylated glycoproteins in living animals, enabling future identification of new biomarkers.
Chang
Pamela V
PV
Department of Chemistry and Howard Hughes Medical Institute, University of California, Berkeley, Berkeley, CA 94720, USA.
Chen
Xing
X
Smyrniotis
Chris
C
Xenakis
Alexander
A
Hu
Tianshun
T
Bertozzi
Carolyn R
CR
Wu
Peng
P
D000818:Animals; D001386:Azides / Q000494:pharmacology*; D016466:CHO Cells; D006224:Cricetinae; D003412:Cricetulus; D006023:Glycoproteins / Q000378:metabolism*; D006595:Hexosamines / Q000494:pharmacology*; D006801:Humans; D019169:Jurkat Cells; D051379:Mice; D012794:Sialic Acids / Q000378:metabolism*
D001386:Azides; D006023:Glycoproteins; D006595:Hexosamines; C405953:N-azidoacetylmannosamine; D012794:Sialic Acids