title: "Signal-on" detection of DNA hole transfer at the single molecule level.
abstract: The DNA base stack provides unique features for the efficient long-range charge transfer. For the purpose of investigating the hole transfer process of individual DNA and the optical readout of DNA information at the single molecule level, we performed single-molecule detection of DNA hole transfer (DNA HT) using single-molecule fluorescence spectroscopy. We have established the single-molecule detection of DNA HT at the single molecule level based on the fluorescence generation by combining the oxidative reaction of the quencher molecule through DNA HT and the cancellation of FRET. This "signal-on" detection system makes it possible to detect DNA HT in individual DNA and the base-pair mismatch in the target DNA with high sensitivity and accuracy.
authors.affiliation: The Institute of Scientific and Industrial Research (SANKEN), Osaka University, Mihogaoka 8-1, Ibaraki, Osaka, Japan.
authors.forename: Tadao
authors.initials: T
authors.lastname: Takada
authors.forename: Yuichiro
authors.initials: Y
authors.lastname: Takeda
authors.forename: Mamoru
authors.initials: M
authors.lastname: Fujitsuka
authors.forename: Tetsuro
authors.initials: T
authors.lastname: Majima
mesh_terms: D001483:Base Sequence; D004247:DNA / Q000737:chemistry*; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods*; D009690:Nucleic Acid Conformation; D012680:Sensitivity and Specificity
chemical_list: D004247:DNA