title: Determination of 17alpha-ethynylestradiol, carbamazepine, diazepam, simvastatin, and oxybenzone in fish livers.
abstract: A method using liquid chromatography/tandem mass spectrometry (LC/MS/MS) was developed for the determination of 17alpha-ethynylestradiol in fish liver; a second method using LC/MS was developed for the determination of carbamazepine, diazepam, simvastatin, and oxybenzone in fish liver. The fish liver samples were extracted and cleaned up by using liquid-liquid extraction and solid-phase extraction before the extracts were analyzed by LC/MS or LC/MS/MS with electrospray negative and positive ionization. Recoveries of the 5 target compounds from spiked catfish liver ranged from 72 +/- 2 to 100 +/- 3%. Limits of quantification for the 5 compounds were between 4.2 and 12.3 ng/g (wet weight). Ten turbot (Pleuronichthys verticalis) liver samples were analyzed; levels of 17alpha-ethynylestradiol, carbamazepine, simvastatin, and oxybenzone were below the detection limits. Diazepam was detected in all 10 fish liver samples at concentrations ranging from 23 to 110 ng/g (wet weight).
authors.affiliation: Mississippi State Chemical Laboratory, PO Box CR, Mississippi State, MS 39762, USA.
authors.forename: Jeong-Wook
authors.initials: JW
authors.lastname: Kwon
authors.forename: Kevin L
authors.initials: KL
authors.lastname: Armbrust
authors.forename: Doris
authors.initials: D
authors.lastname: Vidal-Dorsch
authors.forename: Steven M
authors.initials: SM
authors.lastname: Bay
mesh_terms: D000818:Animals; D001577:Benzophenones / Q000032:analysis* / Q000302:isolation & purification; D002220:Carbamazepine / Q000032:analysis* / Q000302:isolation & purification; D002853:Chromatography, Liquid / Q000379:methods; D003975:Diazepam / Q000032:analysis* / Q000302:isolation & purification; D004997:Ethinyl Estradiol / Q000032:analysis* / Q000302:isolation & purification; D005399:Fishes; D007059:Ictaluridae; D008099:Liver / Q000737:chemistry*; D013058:Mass Spectrometry / Q000379:methods; D008958:Models, Molecular; D019821:Simvastatin / Q000032:analysis* / Q000302:isolation & purification
chemical_list: D001577:Benzophenones; D002220:Carbamazepine; D004997:Ethinyl Estradiol; C005290:oxybenzone; D019821:Simvastatin; D003975:Diazepam