title: Synthesis and biological applications of two novel fluorescent proteins-labeling probes.
abstract: Two novel chlorinated fluoresceins 2',4',5',7'-tetrachloro-6-(5-carboxypentyl)-4,7-dichloro fluorescein succinimidyl ester (1G) and 2',4',5',7'-tetrachloro-6-(3-carboxypropyl)-4,7-dichlorofluorescein succinimidyl ester (2G) were synthesized as fluorescent probes for labeling proteins. Structures of target compounds and intermediates were determined via IR, MS, (1)H NMR and element analysis. The investigation in immunofluorescence histochemistry showed them had strong fluorescence, high photostability and good biocompatibility.
authors.affiliation: Key Laboratory of Synthetic and Natural Functional Molecule Chemistry, Ministry of Education, College of Chemistry & Materials Science, Northwest University, Xi' an, China.
authors.forename: Xiang-Long
authors.initials: XL
authors.lastname: Wu
authors.forename: Min
authors.initials: M
authors.lastname: Tian
authors.forename: Huai-Zhen
authors.initials: HZ
authors.lastname: He
authors.forename: Wei
authors.initials: W
authors.lastname: Sun
authors.forename: Jian-Li
authors.initials: JL
authors.lastname: Li
authors.forename: Zhen
authors.initials: Z
authors.lastname: Shi
mesh_terms: D045744:Cell Line, Tumor; D005452:Fluoresceins / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology; D005456:Fluorescent Dyes / Q000138:chemical synthesis* / Q000737:chemistry; D006801:Humans; D011506:Proteins / Q000737:chemistry*; D013194:Staining and Labeling; D013388:Succinimides / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology
chemical_list: C541970:2',4',5',7'-tetrachloro-6-(5-carboxypentyl)-4,7-dichloro fluorescein succinimidyl ester; D005452:Fluoresceins; D005456:Fluorescent Dyes; D011506:Proteins; D013388:Succinimides