ADP-ribosylation factor like 7 (ARL7) interacts with alpha-tubulin and modulates intracellular vesicular transport.
ADP-ribosylation factor (ARF) like 7 (ARL7, also named ARL4C) is a member of ARL family and recent studies showed that it is involved in the AI-dependent cholesterol secretion process. Yet its biological function remains largely unknown. Using a MALDI-TOF/MS analysis, we identified alpha-tubulin interacted with ARL7. The interaction was confirmed by GST pull-down assay and co-immunoprecipitation in renal carcinoma cell 786-O in which we found the endogenous ARL7 is expressed. This is the second ARL member found interacting with tubulin after ARL8. In addition, ARL7Q72L, a GTP-binding form, promoted the transferrin transport from early endosome to recycling endosome significantly. The above data suggested that ARL7 might modulate the intracellular vesicular transport via interaction with microtubules.
Wei
Shu-mei
SM
Department of Pathology, Second Affiliated Hospital of Zhejiang University, School of Medicine, Jiefang Road 88[#], Hangzhou, Zhejiang Province 310009, China.
Xie
Chuan-gao
CG
Abe
Yasuhito
Y
Cai
Jian-ting
JT
D020727:ADP-Ribosylation Factors / Q000235:genetics / Q000378:metabolism*; D045744:Cell Line, Tumor; D011992:Endosomes; D006801:Humans; D021381:Protein Transport; D012333:RNA, Messenger / Q000096:biosynthesis; D022161:Transport Vesicles / Q000378:metabolism*; D014404:Tubulin / Q000378:metabolism*
D012333:RNA, Messenger; C533522:TUBA1A protein, human; D014404:Tubulin; D020727:ADP-Ribosylation Factors; C541133:ARL4C protein, human