title: Magnetization transfer magic-angle-spinning z-spectroscopy of excised tissues.
abstract: NMR experiments devised to aid in analyses of tissues include magnetization transfer (MT), which can highlight the signals of biological macromolecules through cross-relaxation and/or chemical exchange processes with the bulk (1)H water resonance, and high-resolution magic-angle-spinning (HRMAS) methods, akin to those used in solid-state NMR to introduce additional spectral resolution via the averaging of spin anisotropies. This paper explores the result of combining these methodologies, and reports on MT "z-spectroscopy" between water and cell components in excised tissues under a variety of HRMAS conditions. Main features arising from the resulting (1)H "MTMAS" experiments include strong spinning sideband manifolds centered at the liquid water shift, high-resolution isotropic features coinciding with aliphatic and amide proton resonances, and a second sideband manifold arising as spinning speeds are increased. Interpretations are given for the origin of these various features, including simulations shedding further light onto the nature of MT NMR signals observed for tissue samples. Concurrently, histological examinations are reported validating the limits of HRMAS NMR procedures to the analysis of tissue samples preserved in a number of different ways.
authors.affiliation: Department of Chemical Physics, Weizmann Institute of Science, 76100 Rehovot, Israel.
authors.forename: Reut
authors.initials: R
authors.lastname: Avni
authors.forename: Oren
authors.initials: O
authors.lastname: Mangoubi
authors.forename: Rangeet
authors.initials: R
authors.lastname: Bhattacharyya
authors.forename: Hadassa
authors.initials: H
authors.lastname: Degani
authors.forename: Lucio
authors.initials: L
authors.lastname: Frydman
mesh_terms: D000465:Algorithms; D000818:Animals; D001704:Biopolymers / Q000032:analysis*; D003198:Computer Simulation; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D008280:Magnetics; D051379:Mice; D008954:Models, Biological; D009924:Organ Culture Techniques / Q000379:methods; D011522:Protons; D013048:Specimen Handling / Q000379:methods*; D013113:Spin Labels; D014781:Viscera / Q000737:chemistry*
chemical_list: D001704:Biopolymers; D011522:Protons; D013113:Spin Labels