title: Biomechanical and histological comparison of the influence of oestrogen deficient state on tendon healing potential in rats.
abstract: Thirty-six female Sprague-Dawley rats were divided into two groups: oophrectomised (oestrogen deficient) rats and sham operated (oestrogen maintained) rats. Rats were sacrificed at six, ten, and 14 weeks. The rats were randomly chosen to have biomechanical evaluation on one side and histological evaluation on the other. Biomechanical testing was performed on an Instron machine to measure peak load. Histological sections were evaluated for cell proliferation, collagen-fibre organisation, fibroblast density, angiogenesis, inflammatory cells, chondroid and osseous metaplasia. Compared with the sham operated group, the oophrectomised group showed a lesser average maximum stress (42.9 N/m(2) versus 33.7 N/m(2)) at six weeks, which was significant (p < .05). Succeeding weeks showed no significant biomechanical differences between the two groups. The sham operated group showed greater inflammatory response, which was statistically significant (p < 0.05), and also revealed greater cell proliferation and density. The results of this study revealed that endogenous oestrogen may improve healing of the Achilles tendon in rats.
authors.affiliation: Department of Orthopaedics and Traumatology, Baskent University Faculty of Medicine, Ankara, Turkey. esracirci@hotmail.com
authors.forename: Esra
authors.initials: E
authors.lastname: Circi
authors.forename: Sercan
authors.initials: S
authors.lastname: Akpinar
authors.forename: Cenk
authors.initials: C
authors.lastname: Balcik
authors.forename: Didem
authors.initials: D
authors.lastname: Bacanli
authors.forename: Gulnur
authors.initials: G
authors.lastname: Guven
authors.forename: Rahmi Can
authors.initials: RC
authors.lastname: Akgun
authors.forename: Ismail Cengiz
authors.initials: IC
authors.lastname: Tuncay
mesh_terms: D000125:Achilles Tendon / Q000737:chemistry / Q000293:injuries / Q000473:pathology*; D000818:Animals; D001696:Biomechanical Phenomena; D002111:Calcaneus; D049109:Cell Proliferation; D019902:Chondrocytes / Q000473:pathology; D003094:Collagen / Q000032:analysis; D004195:Disease Models, Animal; D004958:Estradiol / Q000097:blood / Q000172:deficiency*; D005260:Female; D005347:Fibroblasts / Q000473:pathology; D018919:Neovascularization, Physiologic; D009999:Ossification, Heterotopic / Q000473:pathology; D010052:Ovariectomy / Q000379:methods; D051381:Rats; D017207:Rats, Sprague-Dawley; D013194:Staining and Labeling; D013314:Stress, Mechanical; D052256:Tendinopathy / Q000473:pathology; D013708:Tendon Injuries / Q000097:blood / Q000473:pathology* / Q000503:physiopathology; D014945:Wound Healing / Q000502:physiology*
chemical_list: D004958:Estradiol; D003094:Collagen