title: Crystallization and preliminary crystallographic analysis of the cell-surface alginate-binding protein Algp7 isolated from Sphingomonas sp. A1.
abstract: Sphingomonas sp. A1, a Gram-negative bacterium, directly internalizes alginate macromolecules through a mouth-like pit that is present on its cell surface. The alginate-binding protein Algp7, which was found to be localized on the cell surface, contributes to the accumulation of alginate in the pit. Algp7 was crystallized at 293 K by means of the sitting-drop vapour-diffusion method with polyethylene glycol 3350 as a crystallizing agent. Preliminary X-ray analysis showed that the Algp7 crystal belonged to space group P2(1)2(1)2(1), with unit-cell parameters a = 50.1, b = 98.0, c = 100.1 A, and that it diffracted to 2.8 A resolution.
authors.affiliation: Kyoto University, Uji, Japan.
authors.forename: Wataru
authors.initials: W
authors.lastname: Hashimoto
authors.forename: Akihito
authors.initials: A
authors.lastname: Ochiai
authors.forename: Jinshan
authors.initials: J
authors.lastname: He
authors.forename: Takafumi
authors.initials: T
authors.lastname: Itoh
authors.forename: Bunzo
authors.initials: B
authors.lastname: Mikami
authors.forename: Kousaku
authors.initials: K
authors.lastname: Murata
mesh_terms: D001426:Bacterial Proteins / Q000032:analysis* / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D002462:Cell Membrane / Q000737:chemistry / Q000378:metabolism*; D003460:Crystallization; D018360:Crystallography, X-Ray; D008565:Membrane Proteins / Q000032:analysis* / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D020578:Sphingomonas / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D008565:Membrane Proteins