Differentiation of Debaryomyces hansenii and Candida famata by rRNA gene intergenic spacer fingerprinting and reassessment of phylogenetic relationships among D. hansenii, C. famata, D. fabryi, C. flareri (=D. subglobosus) and D. prosopidis: description of D. vietnamensis sp. nov. closely related to D. nepalensis.
The intergenic spacer rDNA amplification and AluI fingerprinting (IGSAF) method detected four distinct groups among 170 Debaryomyces hansenii strains: D. hansenii var. hansenii; Candida famata var. famata; D. hansenii var. fabryi and C. famata var. flareri. IGS sequence comparison of representative strains showed that D. hansenii var. hansenii and C. famata var. famata belonged to one species, whereas D. hansenii var. fabryi and C. famata var. flareri belonged to two different ones. This confirmed the following three species recently reinstated: D. hansenii (=C. famata), Debaryomyces fabryi and Debaryomyces subglobosus (=Candida flareri). Accordingly, growth at 37 degrees C may no longer be used to differentiate D. hansenii from D. fabryi. Riboflavin production is more specific for D. fabryi and D. subglobosus strains. IGSAF identified all the other 17 species of the genus Debaryomyces, six of them sharing with D. hansenii an rRNA gene unit harbouring two 5S rRNA genes. The phylogenetic tree established with IGS sequences was congruent with the one based on ACT1, GPD1 and COX2 sequences depicting a distinct D. hansenii clade close to the D. subglobosus, Debaryomyces prosopidis and D. fabryi clade. Description of Debaryomyces vietnamensis sp. nov. (type strain CBS 10535(T), MUCL 51648(T)), closely related to Debaryomyces nepalensis is given.
Nguyen
Huu-Vang
HV
Collection de Levures d'Intérêt Biotechnologique, Laboratoire de Microbiologie et Génétique Moléculaire, Centre Versailles-Grignon, INRA (UMR1238) CNRS (UMR2585) AgroParisTech, Thiverval-Grignon, France. huu-vang.nguyen@grignon.inra.fr
Gaillardin
Claude
C
Neuvéglise
Cécile
C
D000818:Animals; D002175:Candida / Q000145:classification* / Q000235:genetics*; D016000:Cluster Analysis; D016172:DNA Fingerprinting / Q000379:methods*; D004271:DNA, Fungal / Q000235:genetics*; D021903:DNA, Ribosomal Spacer / Q000235:genetics*; D005516:Food Microbiology; D005838:Genotype; D006801:Humans; D016533:Mycological Typing Techniques; D010802:Phylogeny; D010944:Plants / Q000382:microbiology; D012150:Polymorphism, Restriction Fragment Length; D004718:Saccharomycetales / Q000145:classification* / Q000235:genetics*
D004271:DNA, Fungal; D021903:DNA, Ribosomal Spacer