Ca2+ increases the specific coenzyme Q10 content in Agrobacterium tumefaciens.
Of various metal ions (Ca2+, Cr3+, Cu2+, Fe2+, Mg2+, Mn2+, Ni2+ and Zn2+) added to the culture medium of Agrobacterium tumefaciens at 1 mM, only Ca2+ increased Coenzyme Q10 (CoQ(10)) content in cells without the inhibition of cell growth. In a pH-stat fed-batch culture, supplementation with 40 mM of CaCO3 increased the specific CoQ(10) content and oxidative stress by 22.4 and 48%, respectively. Also, the effect of Ca2+ on the increase of CoQ(10) content was successfully verified in a pilot-scale (300 L) fermentor. In this study, the increased oxidative stress in A. tumefaciens culture by the supplementation of Ca2+ is hypothesized to stimulate the increase of specific CoQ(10) content in order to protect the membrane against lipid peroxidation. Our results improve the understanding of Ca2+ effect on CoQ(10) biosynthesis in A. tumefaciens and should contribute to better industrial production of CoQ(10) by biological processes.
Ha
Suk-Jin
SJ
BioNgene Co., Ltd., 10-1 Myungryun-Dong Chongro-Ku, Seoul, 110-521, Korea.
Kim
Sang-Yong
SY
Seo
Jin-Ho
JH
Jeya
Marimuthu
M
Zhang
Ye-Wang
YW
Ramu
Thangadurai
T
Kim
In-Won
IW
Lee
Jung-Kul
JK
D016960:Agrobacterium tumefaciens / Q000187:drug effects / Q000378:metabolism*; D002118:Calcium / Q000008:administration & dosage*; D004305:Dose-Response Relationship, Drug; D014451:Ubiquinone / Q000031:analogs & derivatives* / Q000378:metabolism
D014451:Ubiquinone; C024989:coenzyme Q10; D002118:Calcium