D-glucose enhanced 5-aminolevulinic acid production in recombinant Escherichia coli culture.
In this study, we introduced a new strategy, feeding D-glucose, to overproduce extracellular 5-aminolevulinic acid (ALA) in the recombinant Escherichia coli. We investigated that the D-glucose concentration is dependent on extracellular ALA production. The results indicated that increasing D-glucose concentration in bacteria culture enhanced final cell density and ALA yield and simultaneously decreased the activities of ALA synthase (ALAS) and ALA dehydratase (ALAD); then, the inhibitory effect of D-glucose on ALAS activity was relieved with the metabolism of D-glucose. when 4.0 g/L D-glucose was added at late exponential phase; 1.46 g/L ALA was achieved in shaking culture, which is 47% or 109% higher than the ALA yields with 30 mM levulinic acid of ALAD inhibitor or no inhibitor. In jar fermenter, final extracellular ALA concentration reached 3.1 g/L by feeding with D-glucose.
Liu
Xiao Xia
XX
School of Biology and Chemical Engineering, Jiaxing University, Jiaxing 314001, China. sunliuxx@163.com
Wang
Lan
L
Wang
Yu Jie
YJ
Cai
Li Ling
LL
D000624:5-Aminolevulinate Synthetase / Q000378:metabolism; D000622:Aminolevulinic Acid / Q000378:metabolism*; D004274:DNA, Recombinant / Q000235:genetics*; D004926:Escherichia coli / Q000166:cytology / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism*; D005110:Extracellular Space / Q000378:metabolism; D005285:Fermentation / Q000187:drug effects; D005818:Genetic Engineering / Q000379:methods*; D005947:Glucose / Q000494:pharmacology*; D006836:Hydro-Lyases / Q000037:antagonists & inhibitors; D012231:Rhizobium / Q000201:enzymology
D004274:DNA, Recombinant; D000622:Aminolevulinic Acid; D000624:5-Aminolevulinate Synthetase; D006836:Hydro-Lyases; D005947:Glucose