Protein oxidation under extremely low frequency electric field in guinea pigs. Effect of N-acetyl-L-cysteine treatment.
Modern age exposes humans to an increasing level of electromagnetic activity in their environment due to overhead power lines and transformers around residential areas. Studies have shown that treatment with antioxidants can suppress the oxidative damage induced by electromagnetic fields in various frequencies of the non-ionizing radiation band. In this study, we detected protein carbonyl content (PCO), advanced oxidation protein products (AOPP) in liver and 3-nitrotyrosine (3-NT) levels in plasma of guinea pigs in order to investigate the effects of N-acetyl-L-cysteine (NAC) administration on oxidative protein damage induced by power frequency electric (E) field (50 Hz, 12 kV/m, 7 days/8 h/day). We also analyzed hepatic hydroxyproline level to study protein synthesis. According to the findings of the present study, no statistically significant changes occurred in PCO, AOPP and 3-NT levels of the guinea pigs that were exposed to the E field with respect to the control group. However, liver hydroxyproline level was significantly diminished in the E field exposure group compared to the control and PCO, hydroxyproline and 3-NT levels changed significantly in the NAC-administrated groups.
Güler
Göknur
G
Gazi Non-Ionizing Radiation Protection Center, Department of Biophysics, Faculty of Medicine, Gazi University, 06500 Ankara, Turkey.
Türközer
Zerrin
Z
Ozgur
Elcin
E
Tomruk
Arin
A
Seyhan
Nesrin
N
Karasu
Cimen
C
D000111:Acetylcysteine / Q000008:administration & dosage*; D000704:Analysis of Variance; D000818:Animals; D004574:Electromagnetic Fields / Q000009:adverse effects*; D016166:Free Radical Scavengers / Q000008:administration & dosage*; D006168:Guinea Pigs; D006909:Hydroxyproline / Q000378:metabolism; D008099:Liver / Q000187:drug effects / Q000378:metabolism* / Q000528:radiation effects; D008297:Male; D010084:Oxidation-Reduction; D050050:Protein Carbonylation / Q000187:drug effects / Q000528:radiation effects; D011506:Proteins / Q000378:metabolism* / Q000528:radiation effects; D011837:Radiation-Protective Agents / Q000008:administration & dosage; D011897:Random Allocation; D014443:Tyrosine / Q000031:analogs & derivatives / Q000097:blood
D016166:Free Radical Scavengers; D011506:Proteins; D011837:Radiation-Protective Agents; C002744:3-nitrotyrosine; D014443:Tyrosine; D006909:Hydroxyproline; D000111:Acetylcysteine