title: In vitro and in vivo antitrypanosomal activities of three peptide antibiotics: leucinostatin A and B, alamethicin I and tsushimycin.
abstract: In the course of our screening for antitrypanosomal compounds from soil microorganisms, as well as from the antibiotics library of the Kitasato Institute for Life Sciences, we found three peptide antibiotics, leucinostatin (A and B), alamethicin I and tsushimycin, which exhibited potent or moderate antitrypanosomal activity. We report here the in vitro and in vivo antitrypanosomal properties and cytotoxicities of leucinostatin A and B, alamethicin I and tsushimycin compared with suramin. We also discuss their possible mode of action. This is the first report of in vitro and in vivo trypanocidal activity of leucinostatin A and B, alamethicin I and tsushimycin.
authors.affiliation: Research Center for Tropical Diseases, Center for Basic Research, Kitasato Institute for Life Sciences and Graduate School of Infectious Control Sciences, Kitasato University, Tokyo, Japan.
authors.forename: Aki
authors.initials: A
authors.lastname: Ishiyama
authors.forename: Kazuhiko
authors.initials: K
authors.lastname: Otoguro
authors.forename: Masato
authors.initials: M
authors.lastname: Iwatsuki
authors.forename: Masahito
authors.initials: M
authors.lastname: Iwatsuki
authors.forename: Miyuki
authors.initials: M
authors.lastname: Namatame
authors.forename: Aki
authors.initials: A
authors.lastname: Nishihara
authors.forename: Kenichi
authors.initials: K
authors.lastname: Nonaka
authors.forename: Yuta
authors.initials: Y
authors.lastname: Kinoshita
authors.forename: Yoko
authors.initials: Y
authors.lastname: Takahashi
authors.forename: Rokuro
authors.initials: R
authors.lastname: Masuma
authors.forename: Kazuro
authors.initials: K
authors.lastname: Shiomi
authors.forename: Haruki
authors.initials: H
authors.lastname: Yamada
authors.forename: Satoshi
authors.initials: S
authors.lastname: Omura
mesh_terms: D000408:Alamethicin / Q000302:isolation & purification / Q000494:pharmacology* / Q000627:therapeutic use; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology* / Q000627:therapeutic use; D023181:Antimicrobial Cationic Peptides; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D005260:Female; D005285:Fermentation; D055666:Lipopeptides / Q000302:isolation & purification / Q000494:pharmacology* / Q000627:therapeutic use; D051379:Mice; D008813:Mice, Inbred ICR; D010143:Paecilomyces / Q000145:classification / Q000378:metabolism*; D010455:Peptides / Q000302:isolation & purification / Q000494:pharmacology* / Q000627:therapeutic use; D010456:Peptides, Cyclic; D013498:Suramin / Q000494:pharmacology; D014344:Trypanocidal Agents; D014346:Trypanosoma brucei brucei / Q000187:drug effects; D016833:Trypanosoma brucei rhodesiense / Q000187:drug effects; D014353:Trypanosomiasis, African / Q000188:drug therapy* / Q000469:parasitology
chemical_list: D000900:Anti-Bacterial Agents; D023181:Antimicrobial Cationic Peptides; D055666:Lipopeptides; D010455:Peptides; D010456:Peptides, Cyclic; D014344:Trypanocidal Agents; C031299:tsushimycin; D000408:Alamethicin; D013498:Suramin; C008268:leucinostatin A; C040316:leucinostatin B