title: Inhibition of LPS-induced proinflammatory responses of J774.2 macrophages by immobilized enzymatically tailored pectins.
abstract: The surface of an implant device can be modified by immobilizing biological molecules on it to improve its integration into the host tissue. We have previously demonstrated that enzymatically tailored plant pectins are promising nanocoatings for biomaterials. This study investigates whether a coating of modified hairy region (rhamnogalacturonan-I) from apple pectin (MHR-alpha) which has anti-adhesive properties can inhibit the generation of inflammatory mediators by lipopolysaccharide (LPS)-activated macrophages. For that purpose, J774.2 murine macrophages were cultured for 24h on MHR-alpha-coated Petri dishes and tissue culture polystyrene controls, with and without LPS. Cell morphology, cell growth, nitrite and TNF-alpha secretion were studied. The results indicate that MHR-alpha coating inhibits the LPS-induced activation of macrophages.
authors.affiliation: UMR CNRS 6600, Biomécanique et Bioingénierie, Université de Technologie de Compiègne, BP 20529, 60205 Compiègne Cedex, France.
authors.forename: M
authors.initials: M
authors.lastname: Gallet
authors.forename: M
authors.initials: M
authors.lastname: Vayssade
authors.forename: M
authors.initials: M
authors.lastname: Morra
authors.forename: R
authors.initials: R
authors.lastname: Verhoef
authors.forename: S
authors.initials: S
authors.lastname: Perrone
authors.forename: G
authors.initials: G
authors.lastname: Cascardo
authors.forename: P
authors.initials: P
authors.lastname: Vigneron
authors.forename: H A
authors.initials: HA
authors.lastname: Schols
authors.forename: M-D
authors.initials: MD
authors.lastname: Nagel
mesh_terms: D000818:Animals; D002460:Cell Line; D016207:Cytokines; D004800:Enzymes, Immobilized / Q000737:chemistry*; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism*; D051379:Mice; D010368:Pectins / Q000737:chemistry* / Q000494:pharmacology*
chemical_list: D016207:Cytokines; D004800:Enzymes, Immobilized; D008070:Lipopolysaccharides; D010368:Pectins