[Effect of konjac polysaccharide on levels of leptin and Na+ -K+-ATPase of mice treated with high fat].
OBJECTIVE
To investigate the effect of konjac polysaccharide on serum leptin and intestinal mucosa Na+ -K+-ATPase activity of mice treated with high fat.


METHODS
Mice were divided into normal control, high-fat control, and high, middle, low dose of konjac polysaccharide combining high fat groups. The mice were feed for twenty days. The levels of serum leptin were determined by ELISA method, and intestinal mucosa Na+ -K+-ATPase activities were determined by spectrophotometry. The body weight, adipose tissue and blood glucose were also examined.


RESULTS
At the 10th day, body weight and postprandial blood glucose of high-fat control group were (31.3 +/- 2.11) g and (7.5 +/- 1.15) mmol/L, those of high dose konjac polysaccharide combining high fat group were(28.0 +/- 2.06) g and (4.8 +/- 0.73) mmol/L. At the 20th day, concentrations of serum leptin and Na+ -K-ATPase activities of high-fat control group were (1078.5 +/- 61.69) pg/ml and (16.2 +/- 1.48) micromol Pi/(mg pro x h), those of high dose konjac polysaccharide combining high fat group were (820.5 +/- 58.52) pg/ml and (11.2 +/- 1.10) micromol Pi/(mg pro x h). All differences between two groups were significant (P < 0.05).


CONCLUSIONS
Konjac polysaccharide could decrease level of body weight, postprandial blood glucose, serum leptin and intestinal mucosa Na -K+-ATPase activity of mice treated with high fat.
Lin
Jianwei
J
Medical College of Qingdao University, Qingdao 266021, China.
Zhong
Jinyi
J
Wang
Shuping
S
D031056:Amorphophallus / Q000737:chemistry*; D000818:Animals; D001786:Blood Glucose / Q000032:analysis; D001835:Body Weight / Q000187:drug effects; D004041:Dietary Fats / Q000008:administration & dosage*; D005260:Female; D007413:Intestinal Mucosa / Q000201:enzymology; D020738:Leptin / Q000097:blood*; D051379:Mice; D011134:Polysaccharides / Q000494:pharmacology*; D011897:Random Allocation; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism*
D001786:Blood Glucose; D004041:Dietary Fats; D020738:Leptin; D011134:Polysaccharides; D000254:Sodium-Potassium-Exchanging ATPase