Tetrazolium violet induces apoptosis via caspases-8, -9 activation and Fas/FasL up-regulation in rat C6 glioma cells.
Tetrazolium violet (TV), a tetrazolium salt, was synthesized as a novel and potent anticancer agent with a broad spectrum of anticancer activity against many cancer cells. A previous study showed that tetrazolium violet inhibited cell growth, and induced cell cycle arrest and apoptosis in C6 Rat glioma cells. It also showed that treatment of cells with TV for 24 h resulted in a dramatic up-regulation of p53, and an increase in the activity of caspase-3, accompanied with a significant increase of Bax/Bcl-2 ratio. In this study, we further investigated which Fas/FasL and caspase were activated by TV during the apoptosis. Annexin-V-propidium iodide (PI) binding assay and nucleosome ELISA assay further indicated that TV induced a typical apoptosis, in a time-dose-dependent manner. The data showed that the activity of Fas/FasL and caspase-8 and -9 were significantly enhanced by the compound, which suggested that TV might be used as a Fas/FasL and caspases promoter to initiate brain cancer cell apoptosis.
Cai
Cheng-Fa
CF
School of Life Science, Shandong University, Jinan, 250100, China.
Feng
Lei
L
Wang
Lei
L
Kong
Qing-Zhong
QZ
Zhao
Yun-Feng
YF
D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D053181:Caspase 8 / Q000378:metabolism*; D053453:Caspase 9 / Q000378:metabolism*; D045744:Cell Line, Tumor; D004305:Dose-Response Relationship, Drug; D004789:Enzyme Activation; D053222:Fas Ligand Protein / Q000378:metabolism*; D005910:Glioma / Q000201:enzymology / Q000276:immunology / Q000473:pathology*; D051381:Rats; D013778:Tetrazolium Salts / Q000494:pharmacology*; D013997:Time Factors; D015854:Up-Regulation; D019014:fas Receptor / Q000378:metabolism*
D000970:Antineoplastic Agents; D053222:Fas Ligand Protein; C491898:Fas protein, rat; D013778:Tetrazolium Salts; D019014:fas Receptor; C531352:tetrazolium violet; C505528:Casp8 protein, rat; C506435:Casp9 protein, rat; D053181:Caspase 8; D053453:Caspase 9