title: Route to smooth silica-based surfaces decorated with novel self-assembled monolayers (SAMs) containing glycidyl-terminated very long hydrocarbon chains.
abstract: Novel glycidyl-terminated organosilicon coupling agents possessing a trialkoxysilyl head group and a very long hydrocarbon chain (C22) were synthesized. Their ability to afford densely packed self-assembled monolayers (SAMs) grafted on silica-based surfaces was investigated. Transmission FT-IR spectra showed that the most regular films were obtained by using trichloracetic acid as the catalyst (10 M%). Atomic force microscopy (AFM) and optical ellipsometry were consistent with well ordered monolayers exhibiting a marked decrease of the surface roughness. Epifluorescence microscopy revealed that these SAMs possessed a better surface reactivity than monolayers obtained with the commercially available (3-glycidoxypropyl) trimethoxysilane (GPTS) upon grafting of a fluorescent probe (dansylcadaverin). Moreover, direct attachment of fluorescent antibodies (RAG-TRITC) through covalent binding led to higher mean fluorescence intensities, showing that these new SAMs possess high potential for the immobilization of biological molecules.
authors.affiliation: Université de Bordeaux, Institut des Sciences Moléculaires, UMR 5255-CNRS, 351 cours de la Libération, 33405 Talence Cedex, France.
authors.forename: Duy Hai
authors.initials: DH
authors.lastname: Dinh
authors.forename: Luc
authors.initials: L
authors.lastname: Vellutini
authors.forename: Bernard
authors.initials: B
authors.lastname: Bennetau
authors.forename: Corinne
authors.initials: C
authors.lastname: Dejous
authors.forename: Dominique
authors.initials: D
authors.lastname: Rebière
authors.forename: Emilie
authors.initials: E
authors.lastname: Pascal
authors.forename: Daniel
authors.initials: D
authors.lastname: Moynet
authors.forename: Colette
authors.initials: C
authors.lastname: Belin
authors.forename: Bernard
authors.initials: B
authors.lastname: Desbat
authors.forename: Christine
authors.initials: C
authors.lastname: Labrugère
authors.forename: Jean-Paul
authors.initials: JP
authors.lastname: Pillot
mesh_terms: D002103:Cadaverine / Q000031:analogs & derivatives / Q000737:chemistry; D002384:Catalysis; D003432:Cross-Linking Reagents / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry; D008567:Membranes, Artificial; D018625:Microscopy, Atomic Force; D012821:Silanes / Q000737:chemistry*; D012822:Silicon Dioxide / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods; D013499:Surface Properties
chemical_list: C403136:3-glycidoxypropyltrimethoxysilane; D003432:Cross-Linking Reagents; D005456:Fluorescent Dyes; D008567:Membranes, Artificial; D012821:Silanes; D012822:Silicon Dioxide; C008542:monodansylcadaverine; D002103:Cadaverine