Target preparation for genotyping specific genes or gene segments.
Generation of single stranded target is of high importance for hybridization reactions on oligonucleotide microarrays. Several methods have been established for production of single stranded DNA and in vitro transcribed RNA. Here we describe three robust methods for target amplification from purified genomic DNA or pre-amplified DNA. The protocols include incorporation of biotin labels in the target molecules and allow for biotin/streptavidin chemistry to be utilized for flexibility in choice of visualization strategy.
Petersen
Jesper
J
Technical University of Denmark, Jyllinge, Denmark.
Poulsen
Lena
L
Dufva
Martin
M
D004247:DNA / Q000235:genetics; D005796:Genes; D005838:Genotype; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D016133:Polymerase Chain Reaction; D012313:RNA / Q000235:genetics; D048348:Reverse Transcription; D013698:Templates, Genetic; D013997:Time Factors
D012313:RNA; D004247:DNA