title: A short cytochemical note on the nucleolar and cytoplasmic RNA concentration in differentiating cells represented by human erythroblasts.
abstract: The present study was undertaken to provide more information on the nucleolar and cytoplasmic RNA concentration in differentiating cells of the erythroid lineage. These cells represent a convenient model to study cell differentiation since all stages are morphologically well characterised. The bone marrow of patients suffering from the chronic phase of chronic myeloid leukaemia without a large increase in the granulocyte to erythroid ratio provided erythroblasts for computer-assisted image density measurements of RNA in nucleoli and cytoplasm at the single cell level. The measurements indicated a significant decrease of the nucleolar and cytoplasmic RNA concentration only in advanced stages of erythroblast differentiation (polychromatic and orthochromatic erythroblasts). The ratio of the nucleolar to cytoplasmic RNA concentration was otherwise very stable and did not change during differentiation, being similar in the early and advanced stages of erythroblastic development. In contrast, the nucleolar size significantly decreased even during the early stages of erythroid development (basophilic erythroblasts). This marked decrease in the nucleolar diameter in differentiating erythroblasts and the less marked decrease in the nucleolar RNA concentration suggest that the amount of RNA in the nucleolus is closely associated with nucleolar size rather than on its concentration within the nucleolar body.
authors.affiliation: Institute of Haematology and Blood Transfusion, Prague 128 20, Czech Republic. karel.smetana@uhkt.cz
authors.forename: K
authors.initials: K
authors.lastname: Smetana
authors.forename: H
authors.initials: H
authors.lastname: Klamová
authors.forename: I
authors.initials: I
authors.lastname: Jirásková
authors.forename: D
authors.initials: D
authors.lastname: Mikulenková
mesh_terms: D002454:Cell Differentiation / Q000235:genetics / Q000502:physiology*; D002466:Cell Nucleolus / Q000235:genetics*; D002478:Cells, Cultured; D003593:Cytoplasm / Q000235:genetics*; D004900:Erythroblasts / Q000378:metabolism*; D006801:Humans; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000235:genetics; D012313:RNA / Q000235:genetics*
chemical_list: D012313:RNA