Prevalence and significance of human parvovirus variants in skin from primary cutaneous T cell lymphomas, inflammatory dermatoses and healthy subjects.
Primary cutaneous T cell lymphomas (CTCL) represent a heterogeneous group of T lymphomas. Virus involvement in CTCL pathogenesis has been extensively investigated, but no data are available as to a causative role of parvovirus B19. The prevalence of parvovirus variants (B19, LaL1/K71, V9) was investigated by using two nested PCRs and a genotype-2 semiquantitative PCR (Q-PCR). Parvovirus DNA was detected in similar percentage in healthy skin controls (40%; n = 42), inflammatory dermatoses (ID) (41%; n = 80) and CTCL (34%; n = 76). Among variants, genotype-2 was more prevalent in ID (26%) and CTCL (22%) than in normal skin (14%; p < 0.05). genotype-3 was never found in normal skin and was rare in ID. The only four pathological skin samples with a quantifiable genome copies/mug DNA values in Q-PCR were ID. In conclusion, despite the skin represent a reservoir for genotype-1, parvovirus infection is not involved in the etiopathogenesis of CTCL.
Sidoti
Francesca
F
Virology Unit, Department of Public Health and Microbiology, Turin University, Turin, Italy.
Fierro
Maria Teresa
MT
Costa
Cristina
C
Ponti
Renata
R
Bergallo
Massimiliano
M
Comessatti
Alessandra
A
Fumagalli
Mara
M
Novelli
Mauro
M
Merlino
Chiara
C
Cavallo
Rossana
R
Bernengo
Maria Grazia
MG
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D004279:DNA, Viral / Q000032:analysis; D003872:Dermatitis / Q000821:virology*; D005260:Female; D006801:Humans; D016410:Lymphoma, T-Cell, Cutaneous / Q000821:virology*; D008297:Male; D008875:Middle Aged; D010322:Parvoviridae Infections / Q000150:complications* / Q000175:diagnosis; D016732:Parvovirus B19, Human / Q000235:genetics / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction; D012867:Skin / Q000821:virology*; D012878:Skin Neoplasms / Q000821:virology*
D004279:DNA, Viral