title: A xenon-129 biosensor for monitoring MHC-peptide interactions.
abstract: Caged in: The formation of a complex between a peptide ligand and a major histocompatibility complex (MHC) class II protein is detected by a (129)Xe biosensor. Cryptophane molecules that trap Xe atoms are modified with a hemagglutinin (HA) peptide, which binds to the MHC protein. The interaction can be monitored by an NMR chemical shift change of cage-HA bound (129)Xe.
authors.affiliation: Protein Engineering Group, Leibniz-Institut für Molekulare Pharmakologie und FU Berlin, Robert-Roessle-Strasse 10, 13125 Berlin, Germany.
authors.forename: Andreas
authors.initials: A
authors.lastname: Schlundt
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Kilian
authors.forename: Michael
authors.initials: M
authors.lastname: Beyermann
authors.forename: Jana
authors.initials: J
authors.lastname: Sticht
authors.forename: Sebastian
authors.initials: S
authors.lastname: Günther
authors.forename: Sabine
authors.initials: S
authors.lastname: Höpner
authors.forename: Kirsten
authors.initials: K
authors.lastname: Falk
authors.forename: Olaf
authors.initials: O
authors.lastname: Roetzschke
authors.forename: Lorenz
authors.initials: L
authors.lastname: Mitschang
authors.forename: Christian
authors.initials: C
authors.lastname: Freund
mesh_terms: D015374:Biosensing Techniques; D002460:Cell Line; D006388:Hemagglutinins / Q000737:chemistry; D000949:Histocompatibility Antigens Class II / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D046911:Macromolecular Substances / Q000737:chemistry; D019906:Nuclear Magnetic Resonance, Biomolecular; D011083:Polycyclic Compounds; D025941:Protein Interaction Mapping / Q000379:methods*; D014230:Triazoles / Q000737:chemistry; D014979:Xenon Isotopes / Q000737:chemistry*
chemical_list: D006388:Hemagglutinins; D000949:Histocompatibility Antigens Class II; D046911:Macromolecular Substances; D011083:Polycyclic Compounds; D014230:Triazoles; D014979:Xenon Isotopes; C523242:cryptophane