Simultaneous expression of homologous and heterologous antigens in rough, attenuated Brucella melitensis.
The possibility of expressing a homologous antigen and a heterologous antigen simultaneously in an attenuated Brucella melitensis strain was investigated. The Brucella wboA gene encoding a mannosyltransferase involved in biosynthesis of lipopolysaccharide O-antigen, and the Bacillus anthracis pag gene encoding the protective antigen (PA) were cloned into plasmid pBBR4MCS. The resulting plasmid was introduced into O-antigen deficient B. melitensis strain WRRP1 to produce strain WRSPA. Strain WRSPA produced O-antigen and a series of PA products, induced protection in BALB/c mice against challenge with B. melitensis strain 16M, but failed to protect A/J mice against challenge with B. anthracis Sterne strain.
Bandara
Aloka B
AB
Center for Molecular Medicine and Infectious Diseases, Virginia-Maryland Regional College of Veterinary Medicine, Virginia Polytechnic Institute and State University, Washington Street, Blacksburg, VA 24061-0910, USA. abandara@vt.edu
Poff-Reichow
Sherry A
SA
Nikolich
Mikeljon
M
Hoover
David L
DL
Sriranganathan
Nammalwar
N
Schurig
Gerhardt G
GG
Dobrean
Victor
V
Boyle
Stephen M
SM
D000818:Animals; D000881:Anthrax / Q000517:prevention & control; D000942:Antigens, Bacterial / Q000096:biosynthesis* / Q000235:genetics; D001408:Bacillus anthracis / Q000235:genetics / Q000276:immunology*; D001427:Bacterial Toxins / Q000096:biosynthesis* / Q000235:genetics; D002004:Brucella Vaccine / Q000235:genetics / Q000276:immunology*; D017347:Brucella melitensis / Q000235:genetics / Q000276:immunology*; D002006:Brucellosis / Q000517:prevention & control; D005260:Female; D051379:Mice; D008807:Mice, Inbred BALB C; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics; D014613:Vaccines, Attenuated / Q000235:genetics / Q000276:immunology
D000942:Antigens, Bacterial; D001427:Bacterial Toxins; D002004:Brucella Vaccine; D011994:Recombinant Proteins; D014613:Vaccines, Attenuated; C030325:anthrax toxin