title: Novel pathologic findings associated with urinary retention in a mouse model of mucopolysaccharidosis type IIIB.
abstract: Mucopolysaccharidosis type IIIB (MPS IIIB; Sanfilippo syndrome type B) is a metabolic disorder with devastating clinical characteristics starting in early childhood and leading to premature death. A knockout mouse strain was developed that models this disease. Mice of the strain B6.129S6- Naglu(tm1Efn)/J are invaluable for investigating pathogenesis and possible treatment modalities. However, the mouse strain also exhibits some objectionable phenotypic features. One such feature, urinary retention, not only is atypical of human MPS IIIB but often leads to early termination of experiments due to animal welfare concerns. The aim of this study was to investigate abnormalities associated with the urinary retention. Necropsies were performed on 9-mo-old mice; urinalysis, hematology and blood chemistry parameters were evaluated, and urogenital specimens were microscopically examined. Histopathologic examinations of urinary tract specimens proved illuminating regarding pathology in the urinary tract. A large mononuclear cell infiltrate was discovered in mutant mice of both sexes, more pronounced in females compared with male mice. The infiltrate comprises of large rounded or polygonal cells with generous variably vacuolated, granular eosinophilic cytoplasm and small round vesicular nuclei. These cells were present throughout and expand the interstitium of the lower urinary tract. Either this results in extrinsic compression of the lumen of the urethra, eventually leading to obstructive uropathy, bladder hyperdistension, and urinary retention or possibly interferes with the neurogenic component of micturition needs to be further investigated. The novel finding of an unexpected mononuclear cell infiltrate in the urinary tract in the knockout mice B6.129S6- Naglu(tm1Efn)/J is reported.
authors.affiliation: Center for Aging and Brain Repair, University of South Florida, College of Medicine, Tampa, Florida, USA. Sylvia.gografe@vanderbilt.edu
authors.forename: Sylvia I
authors.initials: SI
authors.lastname: Gografe
authors.forename: Paul R
authors.initials: PR
authors.lastname: Sanberg
authors.forename: Wilfredo
authors.initials: W
authors.lastname: Chamizo
authors.forename: Hector
authors.initials: H
authors.lastname: Monforte
authors.forename: Svitlana
authors.initials: S
authors.lastname: Garbuzova-Davis
mesh_terms: D000118:Acetylglucosaminidase / Q000235:genetics / Q000378:metabolism; D000818:Animals; D002675:Child, Preschool; D004195:Disease Models, Animal; D005260:Female; D006801:Humans; D007668:Kidney / Q000166:cytology / Q000473:pathology; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D009084:Mucopolysaccharidosis III / Q000150:complications* / Q000473:pathology* / Q000503:physiopathology; D010641:Phenotype; D016482:Urinalysis; D016055:Urinary Retention / Q000209:etiology / Q000473:pathology; D014551:Urinary Tract / Q000473:pathology; D014621:Vagina / Q000166:cytology
chemical_list: C033079:alpha-N-acetyl-D-glucosaminidase; D000118:Acetylglucosaminidase