Modulation of bovine sperm signalling pathways: correlation between intracellular parameters and sperm capacitation and acrosome exocytosis.
In the present study, the viability, intracellular pH (pHi), cAMP ([cAMP]i), calcium concentration and protein phosphotyrosine content were evaluated in relation to the acrosomal and capacitation status of freshly ejaculated bull spermatozoa. These parameters were evaluated before and after incubation with the capacitation inducer heparin, the phosphodiesterase inhibitor 3-isobutyl-1-methylxanthine (IBMX), the phosphotyrosyl-protein phosphatase inhibitors phenylarsine oxide (PAO) and sodium orthovanadate, and hydrogen peroxide. The results obtained were integrated to address the physiological interactions between the different signalling events affecting sperm capacitation and acrosome reaction. As expected, heparin promoted the expression of the 'B' pattern of chlortetracycline binding, increased pHi, [cAMP]i and the phosphotyrosine content of sperm proteins. The effects of heparin were enhanced by IBMX. Both PAO and sodium orthovanadate stimulated protein phosphotyrosine content and acrosomal exocytosis, although only PAO affected pH, Ca2+ and cAMP levels. Intracellular pH was increased while both Ca2+ and [cAMP]i were decreased. Physiological concentrations of H2O2 increased [cAMP]i and promoted acrosomal exocytosis. A significant positive correlation was found between sperm capacitation, protein phosphotyrosine content and stored Ca2+ concentration, whereas the acrosome reaction was correlated with pHi and Ca2+ concentration. This study presents the first global analysis of the major elements individually described during sperm capacitation and acrosome reaction signalling pathways, supported by statistical correlations.
Pons-Rejraji
Hanae
H
Département d'Obstétrique-Gynécologie, Centre de Recherche en Biologie de la Reproduction, Université Laval, Québec, QC G1V 4G2, Canada.
Bailey
Janice L
JL
Leclerc
Pierre
P
D015056:1-Methyl-3-isobutylxanthine / Q000494:pharmacology; D020101:Acrosome Reaction / Q000187:drug effects; D000818:Animals; D001152:Arsenicals / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism; D002417:Cattle; D002470:Cell Survival; D002751:Chlortetracycline / Q000378:metabolism; D000242:Cyclic AMP / Q000378:metabolism; D005089:Exocytosis / Q000187:drug effects; D006493:Heparin / Q000494:pharmacology; D006861:Hydrogen Peroxide / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D008297:Male; D010726:Phosphodiesterase Inhibitors / Q000494:pharmacology; D019000:Phosphotyrosine / Q000378:metabolism; D017027:Protein Tyrosine Phosphatases / Q000037:antagonists & inhibitors / Q000378:metabolism; D012099:Reproductive Techniques / Q000662:veterinary; D015398:Signal Transduction / Q000187:drug effects; D013075:Sperm Capacitation / Q000187:drug effects; D013094:Spermatozoa / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D013997:Time Factors; D014638:Vanadates / Q000494:pharmacology
D001152:Arsenicals; D010726:Phosphodiesterase Inhibitors; C029341:oxophenylarsine; D019000:Phosphotyrosine; D014638:Vanadates; D006493:Heparin; D006861:Hydrogen Peroxide; D000242:Cyclic AMP; D017027:Protein Tyrosine Phosphatases; D002118:Calcium; D015056:1-Methyl-3-isobutylxanthine; D002751:Chlortetracycline