The role of heparan sulfate on adhesion of 47 and 51 kDa outer membrane proteins of Helicobacter pylori to gastric cancer cells.
Helicobacter pylori is a common gastrointestinal pathogenic bacterium in humans and the usual preference for the stomach's outer membrane proteins (OMPs) are antigens involved in the adhesion process. Through SDS-PAGE and blotting analyses, using horseradish peroxidase-labeled heparan sulfate (HRP-HS) as a probe, we identified H. pylori OMPs with affinity for heparan sulfate (OMP-HS). Biotin-streptavidin bacterial-adhesion assay was used to evaluate participation of OMP-HS in the adhesion of H. pylori to semi-confluent HeLa S3 and Kato III cell monolayers. The results provide evidence that induction of antibodies against 2 OMP-HSs (HSBP-47 and HSBP-51) could reduce binding of H. pylori to both cell lines and induce detachment of cell-bound bacteria from infected cultured cells.
López-Bolaños
Claudia C
CC
Centro de Investigaciones Biologicas del Noroeste, Mar Bermejo 195, Col. Playa Palo de Santa Rita, La Paz, B.C.S. 23090, Mexico.
Guzmán-Murillo
Maria A
MA
Ruiz-Bustos
Eduardo
E
Ascencio
Felipe
F
D001422:Bacterial Adhesion / Q000187:drug effects*; D001425:Bacterial Outer Membrane Proteins / Q000378:metabolism*; D045744:Cell Line, Tumor; D005753:Gastric Mucosa / Q000382:microbiology*; D006367:HeLa Cells; D016480:Helicobacter pylori / Q000187:drug effects / Q000502:physiology*; D006497:Heparan Sulfate / Q000494:pharmacology*; D006801:Humans
D001425:Bacterial Outer Membrane Proteins; D006497:Heparan Sulfate