Identification of conserved erythrocyte binding regions in members of the Plasmodium falciparum Cys6 lipid raft-associated protein family.
Detergent-resistant lipid raft membrane-associated Pf12, Pf38 and Pf41 proteins belong to the Cys(6) family, whose members are implicated in Plasmodium falciparum invasion to erythrocytes. We have analyzed the interaction between 20-mer-long synthetic peptides spanning the entire Pf12, Pf38 and Pf41 sequences and erythrocytes. Eight high-activity binding peptides (HABPs) were identified in these proteins, which presented saturable bindings susceptible to erythrocytes' enzymatic treatment, and beta-turn, random coil and alpha-helical elements as principal structural features. Some of these HABPs inhibited merozoite invasion in vitro, suggesting a possible role of Pf12, Pf38 and Pf41 during erythrocyte invasion and supporting their inclusion in the design of a fully effective antimalarial vaccine.
García
Jeison
J
Fundación Instituto de Inmunología de Colombia FIDIC, Bogotá, Colombia.
Curtidor
Hernando
H
Pinzón
Carlos G
CG
Vanegas
Magnolia
M
Moreno
Armando
A
Patarroyo
Manuel E
ME
D000818:Animals; D000953:Antigens, Protozoan / Q000235:genetics*; D004912:Erythrocytes / Q000469:parasitology; D006801:Humans; D010963:Plasmodium falciparum / Q000235:genetics*; D011485:Protein Binding; D025941:Protein Interaction Mapping; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D015800:Protozoan Proteins / Q000235:genetics*; D011956:Receptors, Cell Surface / Q000235:genetics*
D000953:Antigens, Protozoan; C066001:Duffy antigen binding protein, Plasmodium; C507527:Pf12 antigen, Plasmodium falciparum; C507528:Pf38 antigen, Plasmodium falciparum; C507529:Pf41 antigen, Plasmodium falciparum; D015800:Protozoan Proteins; D011956:Receptors, Cell Surface