[Suppressive effects of GTW treatment on infiltration of inflammatory cell in glomeruli in anti-Thy1.1 glomerulonephritis].
OBJECTIVE
To examine inhibition action of multi-glycoside of Tripterygium wilfordii (GTW) on infiltration of inflammatory cell in glomeruli with anti-Thy1.1 glomerulonephritis (anti-Thy1.1 GN), and to clarify its effects on inflammatory in vitro.


METHODS
Two types of anti-Thy1.1 GN were induced in rats by a single or two intravenous injections with 500 microg of anti-Thy1.1 mAb 1-22-3. Rats were randomly divided into two groups, the GTW group and control group, and sacrificed on day 7 or on day 42 after induction of anti-Thy1.1 GN. Daily oral administration of different dose of GTW and distilled water as a control was started from 3 days before injection or at the same time of injection till the day of sacrifice. Proteinuria was determined during days 7 or during days 42. Infiltration of macrophage and T lymphocyte in glomeruli and mRNA expression of interleukin (IL)-2 and interferon (IFN)-gamma in renal tissue were examined.


RESULTS
Increase of infiltration of macrophage in reversible anti-Thy1.1 GN model, glomerular macrophage infiltration and IL-2 mRNA expansion were attenuated by higher dose of GTW (75 mg x kg(-1) x d(-1)), and increased accumulation of activated macrophage and T lymphocyte in irreversible anti-Thy1.1 GN model, accumulation of macrophage and T lymphocyte in glomeruli and mRNA expansion of IL-2 and IFN-gamma were decreased by middling dose of GTW (50 mg x kg(-1) x d(-1)) as well. Proteinuria was significantly ameliorated after GTW administration.


CONCLUSIONS
The findings suggested that different dose of GTW can ameliorate infiltration of inflammatory cell in glomeruli with anti-Thy1.1 glomerulonephritis in vitro by decreasing the expression of IL-2 and IFN-gamma.
Wan
Yigang
Y
The Department of Traditional Chinese Medicine, The Affiliated Drum Tower Hospital of Nanjing University Medical School, Nanjing 210008, China. wyg68918@sina.com
Sun
Wei
W
Che
Xiaoyan
X
Yang
Haiming
H
Ge
Ming
M
Dai
Wei
W
Shimizu
Fujio
F
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D004305:Dose-Response Relationship, Drug; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects; D005921:Glomerulonephritis / Q000276:immunology / Q000378:metabolism / Q000473:pathology* / Q000503:physiopathology*; D006027:Glycosides / Q000494:pharmacology*; D007249:Inflammation / Q000378:metabolism / Q000473:pathology* / Q000503:physiopathology*; D016898:Interferon-alpha / Q000235:genetics; D007376:Interleukin-2 / Q000235:genetics; D007678:Kidney Glomerulus / Q000187:drug effects* / Q000473:pathology; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D013601:T-Lymphocytes / Q000187:drug effects / Q000378:metabolism; D029757:Tripterygium / Q000737:chemistry*
D000911:Antibodies, Monoclonal; D006027:Glycosides; D016898:Interferon-alpha; D007376:Interleukin-2; D012333:RNA, Messenger