title: Greater transport efficiencies of the membrane fatty acid transporters FAT/CD36 and FATP4 compared with FABPpm and FATP1 and differential effects on fatty acid esterification and oxidation in rat skeletal muscle.
abstract: In selected mammalian tissues, long chain fatty acid transporters (FABPpm, FAT/CD36, FATP1, and FATP4) are co-expressed. There is controversy as to whether they all function as membrane-bound transporters and whether they channel fatty acids to oxidation and/or esterification. Among skeletal muscles, the protein expression of FABPpm, FAT/CD36, and FATP4, but not FATP1, correlated highly with the capacities for oxidative metabolism (r>or=0.94), fatty acid oxidation (r>or=0.88), and triacylglycerol esterification (r>or=0.87). We overexpressed independently FABPpm, FAT/CD36, FATP1, and FATP4, within a normal physiologic range, in rat skeletal muscle, to determine the effects on fatty acid transport and metabolism. Independent overexpression of each fatty acid transporter occurred without altering either the expression or plasmalemmal content of other fatty acid transporters. All transporters increased fatty acid transport, but FAT/CD36 and FATP4 were 2.3- and 1.7-fold more effective than FABPpm and FATP1, respectively. Fatty acid transporters failed to alter the rates of fatty acid esterification into triacylglycerols. In contrast, all transporters increased the rates of long chain fatty acid oxidation, but the effects of FABPpm and FAT/CD36 were 3-fold greater than for FATP1 and FATP4. Thus, fatty acid transporters exhibit different capacities for fatty acid transport and metabolism. In vivo, FAT/CD36 and FATP4 are the most effective fatty acid transporters, whereas FABPpm and FAT/CD36 are key for stimulating fatty acid oxidation.
authors.affiliation: From the Department of Human Health and Nutritional Sciences, University of Guelph, Guelph, Ontario N1G 2W1, Canada.
authors.forename: James G
authors.initials: JG
authors.lastname: Nickerson
authors.affiliation: Department of Laboratory Medical Sciences, Hashemite University, Zarqa 13115, Jordan.
authors.forename: Hakam
authors.initials: H
authors.lastname: Alkhateeb
authors.affiliation: From the Department of Human Health and Nutritional Sciences, University of Guelph, Guelph, Ontario N1G 2W1, Canada.
authors.forename: Carley R
authors.initials: CR
authors.lastname: Benton
authors.affiliation: From the Department of Human Health and Nutritional Sciences, University of Guelph, Guelph, Ontario N1G 2W1, Canada.
authors.forename: James
authors.initials: J
authors.lastname: Lally
authors.affiliation: From the Department of Human Health and Nutritional Sciences, University of Guelph, Guelph, Ontario N1G 2W1, Canada.
authors.forename: Jennifer
authors.initials: J
authors.lastname: Nickerson
authors.affiliation: From the Department of Human Health and Nutritional Sciences, University of Guelph, Guelph, Ontario N1G 2W1, Canada.
authors.forename: Xiao-Xia
authors.initials: XX
authors.lastname: Han
authors.affiliation: From the Department of Human Health and Nutritional Sciences, University of Guelph, Guelph, Ontario N1G 2W1, Canada.
authors.forename: Meredith H
authors.initials: MH
authors.lastname: Wilson
authors.affiliation: From the Department of Human Health and Nutritional Sciences, University of Guelph, Guelph, Ontario N1G 2W1, Canada.
authors.forename: Swati S
authors.initials: SS
authors.lastname: Jain
authors.affiliation: From the Department of Human Health and Nutritional Sciences, University of Guelph, Guelph, Ontario N1G 2W1, Canada.
authors.forename: Laelie A
authors.initials: LA
authors.lastname: Snook
authors.affiliation: Department of Molecular Genetics, Maastricht University, 6200 MD Maastricht, The Netherlands.
authors.forename: Jan F C
authors.initials: JFC
authors.lastname: Glatz
authors.affiliation: Department of Physiology, Medical University of Bialystok, 15-222 Bialystok, Poland.
authors.forename: Adrian
authors.initials: A
authors.lastname: Chabowski
authors.affiliation: Department of Molecular Genetics, Maastricht University, 6200 MD Maastricht, The Netherlands.
authors.forename: Joost J F P
authors.initials: JJFP
authors.lastname: Luiken
authors.affiliation: From the Department of Human Health and Nutritional Sciences, University of Guelph, Guelph, Ontario N1G 2W1, Canada. Electronic address: abonen@uoguelph.ca.
authors.forename: Arend
authors.initials: A
authors.lastname: Bonen
mesh_terms: D000818:Animals; D018955:CD36 Antigens / Q000235:genetics / Q000378:metabolism*; D050612:Fatty Acid Transport Proteins / Q000235:genetics / Q000378:metabolism*; D050556:Fatty Acid-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D005227:Fatty Acids / Q000378:metabolism*; D005260:Female; D015870:Gene Expression / Q000502:physiology; D018482:Muscle, Skeletal / Q000378:metabolism*; D010084:Oxidation-Reduction; D051381:Rats; D017207:Rats, Sprague-Dawley; D014280:Triglycerides / Q000378:metabolism
chemical_list: D018955:CD36 Antigens; C008132:Cd36 protein, rat; D050612:Fatty Acid Transport Proteins; D050556:Fatty Acid-Binding Proteins; D005227:Fatty Acids; C515999:Got2 protein, rat; C493570:Slc27a1 protein, rat; C542875:Slc27a4 protein, rat; D014280:Triglycerides