title: Taking advantage of nonspecific trypsin cleavages for the identification of seed storage proteins in cereals.
abstract: The lack of basic amino acids in seed storage proteins has resulted in the proposal to use chymotrypsin in their study. A comparative study of trypsin and chymotrypsin digestion initially confirmed this preference; however, reanalysis of the trypsin data set defining the specificity as 'semitrypsin' provided enough extra data to bridge the gap between both proteases. Rationale as to why numerous semitryptic peptides are observed in the study of these proteins is provided.
authors.affiliation: Centre de Recherche Public-Gabriel Lippmann, Department 'Environment and Agro-biotechnologies' (EVA), 41, rue du Brill, 4422 Belvaux, Luxembourg. Sergeant@lippmann.lu
authors.forename: Kjell
authors.initials: K
authors.lastname: Sergeant
authors.forename: Carla
authors.initials: C
authors.lastname: Pinheiro
authors.forename: Jean-François
authors.initials: JF
authors.lastname: Hausman
authors.forename: Cândido Pinto
authors.initials: CP
authors.lastname: Ricardo
authors.forename: Jenny
authors.initials: J
authors.lastname: Renaut
mesh_terms: D000595:Amino Acid Sequence; D002918:Chymotrypsin / Q000378:metabolism*; D003198:Computer Simulation; D015180:Electrophoresis, Gel, Two-Dimensional; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000737:chemistry / Q000378:metabolism; D040901:Proteomics / Q000379:methods; D055314:Seed Storage Proteins / Q000737:chemistry* / Q000378:metabolism; D012639:Seeds / Q000737:chemistry*; D014357:Trypsin / Q000378:metabolism*; D003313:Zea mays / Q000737:chemistry* / Q000378:metabolism
chemical_list: D010446:Peptide Fragments; D055314:Seed Storage Proteins; D002918:Chymotrypsin; D014357:Trypsin