title: Electrochemistry of myoglobin in Nafion and multi-walled carbon nanotubes modified carbon ionic liquid electrode.
abstract: The direct electrochemistry of myoglobin (Mb) entrapped in the Nafion film on a multi-walled carbon nanotubes (MWCNTs) modified carbon ionic liquid electrode (CILE) had been investigated in this paper. By using a hydrophilic ionic liquid of 1-ethyl-3-methylimidazolium tetrafluoroborate (EMIMBF(4)) as the modifier, a high-performance basal electrode was fabricated and further modified by MWCNTs, Mb and Nafion by a step-by-step casting method. Spectroscopic results indicated that the Mb molecule on the surface of MWCNTs/CILE retained its native structure. Cyclic voltammetric results showed that a pair of well-defined quasi-reversible redox peaks appeared in the pH 7.0 phosphate buffer solution (PBS), which was attributed to the direct electron transfer of Mb heme Fe(III)/Fe(II) redox couples with the modified electrode. The Nafion/Mb/MWCNTs/CILE gave excellent electrocatalytic activity towards different substrates including trichloroacetic acid (TCA), hydrogen peroxide (H(2)O(2)) and sodium nitrite (NaNO(2)).
authors.affiliation: Key Laboratory of Eco-Chemical Engineering of Ministry of Education, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, PR China. sunwei@qust.edu.cn
authors.forename: Wei
authors.initials: W
authors.lastname: Sun
authors.forename: Xiaoqing
authors.initials: X
authors.lastname: Li
authors.forename: Yan
authors.initials: Y
authors.lastname: Wang
authors.forename: Xia
authors.initials: X
authors.lastname: Li
authors.forename: Changzhi
authors.initials: C
authors.lastname: Zhao
authors.forename: Kui
authors.initials: K
authors.lastname: Jiao
mesh_terms: D000818:Animals; D055162:Biocatalysis; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D004563:Electrochemistry / Q000295:instrumentation / Q000379:methods*; D004566:Electrodes; D005465:Fluorocarbon Polymers / Q000737:chemistry*; D006861:Hydrogen Peroxide / Q000032:analysis; D007093:Imidazoles / Q000737:chemistry*; D009211:Myoglobin / Q000737:chemistry*; D037742:Nanotubes, Carbon / Q000737:chemistry* / Q000648:ultrastructure; D010084:Oxidation-Reduction; D015203:Reproducibility of Results; D012977:Sodium Nitrite / Q000032:analysis; D013057:Spectrum Analysis; D014238:Trichloroacetic Acid / Q000032:analysis
chemical_list: C499119:1-ethyl-3-methylimidazolium tetrafluoroborate; D005465:Fluorocarbon Polymers; D007093:Imidazoles; D009211:Myoglobin; D037742:Nanotubes, Carbon; C040402:perfluorosulfonic acid; D014238:Trichloroacetic Acid; D006861:Hydrogen Peroxide; D012977:Sodium Nitrite