Effects of Baicalin and Octreotide on the serum TNF-alpha level and apoptosis in multiple organs of rats with severe acute pancreatitis.
We investigated the effects of Baicalin and Octreotide on the levels of endotoxin and TNF-alpha in blood and the effects of apoptotic changes in multiple organs of SAP rats, and explored the underlying therapeutic mechanisms of Baicalin and Octreotide. In this study, 135 SAP rats were randomly divided into model control, Baicalin treated and Octreotide treated group (n = 45), respectively, the same number of normal rats were included in sham-operated group (n = 45). The above-mentioned groups were further subdivided into 3, 6 and 12 h subgroups, respectively (15 rats in each subgroup). At 3, 6 and 12 h after operation, the mortality rate of rats, endotoxin and TNF-alpha levels in blood as well as the pathological severity scores, expression levels of Bax protein and apoptosis indexes in multiple organs were determined. Compared to model control group (1),both drugs can relieve the pathological injuries of multiple organs and decrease significantly the levels of endotoxin and TNF-alpha in blood and the mortality rate of rats in treated groups (P < 0.05 or P < 0.01); (2) the expression of Bax protein was upregulated in pancreas, lung, intestinal mucosa (P < 0.05 or P < 0.01) but downregulated in spleen and lymph nodes (P < 0.001 and P < 0.05, respectively) in Baicalin treated group; The apoptosis indexes significantly increased in pancreas, intestinal mucosa, lymph nodes and spleen (P < 0.05 or P < 0.01). (3) the expression of Bax protein was upregulated in pancreas and lung but downregulated in spleen and lymph nodes (P < 0.05 or P < 0.01) in Octreotide treated group; The apoptosis indexes significantly increased in lymph nodes and spleen in Octreotide treated group (P < 0.05 or P < 0.01). Baicalin and Octreotide share a similar therapeutic efficacy in the treatment of SAP via a mechanism that is associated with inhibiting the levels of TNF-alpha in blood and induce apoptosis in multiple organs.
Tian
Hua
H
Department of General Surgery, Affiliated Xiaoshan First People's Hospital, Hangzhou Normal University, Hangzhou, Zhejiang Province, China.
Zhang
Xiping
X
Wu
Chengjun
C
Chen
Li
L
Ying
Rongchao
R
Ye
Jing
J
Yu
Bingyan
B
Ye
Qian
Q
Pan
Yan
Y
Ma
Meili
M
Zhu
Feibo
F
D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal; D017209:Apoptosis / Q000187:drug effects*; D004731:Endotoxins / Q000097:blood; D005419:Flavonoids / Q000494:pharmacology*; D005765:Gastrointestinal Agents; D008297:Male; D015282:Octreotide / Q000494:pharmacology*; D009928:Organ Specificity; D010195:Pancreatitis / Q000150:complications / Q000188:drug therapy* / Q000473:pathology; D051381:Rats; D017207:Rats, Sprague-Dawley; D015996:Survival Rate; D014018:Tissue Distribution; D016896:Treatment Outcome; D014409:Tumor Necrosis Factor-alpha / Q000097:blood / Q000187:drug effects*; D051028:bcl-2-Associated X Protein / Q000032:analysis
D000894:Anti-Inflammatory Agents, Non-Steroidal; D004731:Endotoxins; D005419:Flavonoids; D005765:Gastrointestinal Agents; D014409:Tumor Necrosis Factor-alpha; D051028:bcl-2-Associated X Protein; C038044:baicalin; D015282:Octreotide