Vaccination strategies to enhance local immunity and protection against Mycobacteriun tuberculosis.
To determine the immunogenicity and protective efficacy of the Mycobacterium tuberculosis 10 kD culture filtrate protein (CFP10), and to evaluate strategies that enhance local immunity, we used C57Bl/6 DR4 mice that were transgenic for human HLA DRB1 0401, because CFP10 contains epitopes for DRB1 0401 but not for C57Bl/6 mice. Intramuscular immunization with a DNA vaccine encoding CFP10 elicited production of IFN-gamma by systemic CD4+ T cells, and one intravenous dose of the CFP10-based DNA vaccine coated with polyethylenimine (PEI) stimulated IFN-gamma production by lung CD4+ cells and reduced the pulmonary bacillary burden. We conclude that CFP10 is a potential vaccine candidate and that coating vaccines with PEI enhances local protective immunity to tuberculosis
Klucar
Peter
P
Center for Pulmonary and Infectious Disease Control, University of Texas Health Science Center at Tyler, Tyler, TX 75708, United States.
Barnes
Peter F
PF
Kong
Ying
Y
Howard
Susan T
ST
Pang
Xiuhua
X
Huang
Fang-Fang
FF
Tvinnereim
Amy R
AR
Samten
Buka
B
Shams
Homayoun
H
D000818:Animals; D000942:Antigens, Bacterial / Q000235:genetics / Q000276:immunology; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D015798:HLA-DR1 Antigen / Q000276:immunology; D007114:Immunization; D007371:Interferon-gamma / Q000276:immunology; D008168:Lung / Q000166:cytology / Q000276:immunology; D052119:Lysosomal-Associated Membrane Protein 2 / Q000276:immunology; D051907:Lysosomal Membrane Proteins / Q000276:immunology; D008247:Lysosomes / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D009169:Mycobacterium tuberculosis / Q000276:immunology*; D010446:Peptide Fragments / Q000276:immunology; D010957:Plasmids / Q000276:immunology; D011094:Polyethyleneimine / Q000737:chemistry; D013154:Spleen / Q000166:cytology / Q000276:immunology; D014397:Tuberculosis, Pulmonary / Q000276:immunology* / Q000517:prevention & control*; D019444:Vaccines, DNA / Q000276:immunology; D014614:Vaccines, Synthetic / Q000276:immunology; D052118:Lysosomal-Associated Membrane Protein 1
D000942:Antigens, Bacterial; D015798:HLA-DR1 Antigen; D007371:Interferon-gamma; D052119:Lysosomal-Associated Membrane Protein 2; D051907:Lysosomal Membrane Proteins; D010446:Peptide Fragments; D011094:Polyethyleneimine; D019444:Vaccines, DNA; D014614:Vaccines, Synthetic; C489634:CFP-10 protein (71-85), Mycobacterium tuberculosis; C052255:Lamp1 protein, mouse; D052118:Lysosomal-Associated Membrane Protein 1