Pulmonary disease in hamsters infected with Leptospira interrogans: histopathologic findings and cytokine mRNA expressions.
Our aim was to evaluate the pulmonary changes induced by Leptospira interrogans infection in hamsters, and the gene expression of endogenous mediators in lung fragments during 28 days of observation. The animals were euthanized on days 4, 7, 14, 21, and 28 post-inoculation. Histopathologic lung analysis showed hemorrhage, pneumonia, alveolar congestion, and infiltrated cellular areas, with increasing severity until day 21 post-inoculation. Tumor necrosis factor (TNF)-alpha mRNA expression enhanced in first days with peak on day 4 and slightly decreased in the final phase. The interleukin (IL)-10 remained relatively constant throughout the period, with the exceptions of days 4 and 14. The endothelial nitric-oxide synthesis (eNOS) showed an increased expression on day 4, followed by an augment on days 7 and 14, and remaining constant up to day 28 post-infection. Our results demonstrate that inoculation of L. interrogans sorovar Icterohaemorrhagiae induced pulmonary lesions, including pulmonary hemorrhage, supporting that the lung is a target organ.
Marinho
Márcia
M
Department of Animal Health and Production, Microbiology Laboratory FOA, Unesp-Estadual Paulista University, Araçatuba, São Paulo, Brazil. mmarinho@fmva.unesp.br
Oliveira-Júnior
Itamar Souza
IS
Monteiro
Cristina Maria Rodrigues
CM
Perri
Silvia Helena
SH
Salomão
Reinaldo
R
D000818:Animals; D006224:Cricetinae; D016207:Cytokines / Q000235:genetics / Q000378:metabolism*; D005786:Gene Expression Regulation / Q000502:physiology; D007921:Leptospira interrogans; D007922:Leptospirosis / Q000382:microbiology* / Q000473:pathology*; D008168:Lung / Q000378:metabolism / Q000382:microbiology / Q000473:pathology; D008171:Lung Diseases / Q000382:microbiology* / Q000473:pathology; D008647:Mesocricetus; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism
D016207:Cytokines; D012333:RNA, Messenger