title: Flavobacterium sasangense sp. nov., isolated from a wastewater stream polluted with heavy metals.
abstract: A Gram-negative, rod-shaped bacterium, designated strain YC6274(T), was isolated from a stream (Sasang) carrying wastewater polluted with heavy metals in Busan, Korea. Growth was observed at 10-35 degrees C (optimum, 30 degrees C) and pH 6.0-9.5 (optimum, pH 7.5-8.0). Comparative 16S rRNA gene sequence analyses showed that the strain was most closely related to Flavobacterium cucumis R2A45-3(T) (96.6 % similarity), F. aquatile ATCC 11947(T) (93.7 %), F. croceum EMB47(T) (93.3 %), F. indicum GPTSA100-9(T) (93.3 %) and F. terrigena DS-20(T) (93.2 %). Sequence similarities with strains of other Flavobacterium species with validly published names were lower than 93.0 %. Phylogenetic analysis based on the 16S rRNA gene sequence showed that strain YC6274(T) formed a distinct phyletic lineage within the genus Flavobacterium. The predominant fatty acids of strain YC6274(T) were iso-C(15 : 0), iso-C(16 : 0), iso-C(15 : 1) G, iso-C(17 : 0) 3-OH, iso-C(16 : 0) 3-OH and iso-C(15 : 0) 3-OH. The G+C content of the genomic DNA was 32.5 mol% and the major quinone was MK-6. On the basis of phenotypic, chemotaxonomic and molecular data, it is clear that strain YC6274(T) represents a novel species within the genus Flavobacterium, for which the name Flavobacterium sasangense sp. nov. is proposed. The type strain is YC6274(T) (=KCTC 22246(T) =DSM 21067(T)).
authors.affiliation: Division of Applied Life Science (BK21), PMBBRC and EB-NCRC, Gyeongsang National University, Jinju 660-701, Republic of Korea.
authors.forename: Hwan Sik
authors.initials: HS
authors.lastname: Yoon
authors.forename: Zubair
authors.initials: Z
authors.lastname: Aslam
authors.forename: Geun Cheol
authors.initials: GC
authors.lastname: Song
authors.forename: Seon Won
authors.initials: SW
authors.lastname: Kim
authors.forename: Che Ok
authors.initials: CO
authors.lastname: Jeon
authors.forename: Tae Soo
authors.initials: TS
authors.lastname: Chon
authors.forename: Young Ryun
authors.initials: YR
authors.lastname: Chung
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000032:analysis; D004275:DNA, Ribosomal / Q000032:analysis; D005227:Fatty Acids / Q000032:analysis; D005417:Flavobacterium / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology; D020459:Genes, rRNA; D007220:Industrial Waste; D007723:Korea; D019216:Metals, Heavy; D008969:Molecular Sequence Data; D010641:Phenotype; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D045483:Rivers / Q000737:chemistry* / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D013045:Species Specificity; D014877:Water Pollution, Chemical
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D007220:Industrial Waste; D019216:Metals, Heavy; D012336:RNA, Ribosomal, 16S