Copper-free "click" modification of DNA via nitrile oxide-norbornene 1,3-dipolar cycloaddition.
Nitrile oxides react smoothly and rapidly with norbornene-modified DNA in a copper-free click reaction. The reaction allows high density functionalization of oligodeoxyribonucleotides (ODNs) with a large variety of molecules directly on solid supports and even in synthesizers without the need for an additional catalyst.
Gutsmiedl
Katrin
K
Center for Integrative Protein Science at the Department of Chemistry and Biochemistry, Ludwig Maximilians University, Butenandtstr 5-13, 81377 Munich.
Wirges
Christian T
CT
Ehmke
Veronika
V
Carell
Thomas
T
D002384:Catalysis; D004247:DNA / Q000737:chemistry*; D015394:Molecular Structure; D009570:Nitriles / Q000737:chemistry*; D009636:Norbornanes / Q000737:chemistry; D009838:Oligodeoxyribonucleotides / Q000737:chemistry*; D010087:Oxides / Q000737:chemistry*
D009570:Nitriles; D009636:Norbornanes; D009838:Oligodeoxyribonucleotides; D010087:Oxides; D004247:DNA