title: Bio-Mos: an effective inducer of dicentracin gene expression in European sea bass (Dicentrarchus labrax).
abstract: Concern over the use of dietary antibiotics in aquaculture has encouraged the industry to search for alternatives that both enhance performance and afford protection from disease. Bio-Mos, derived from the outer cell wall of a specific strain of yeast Saccharomyces cerevisiae (Alltech Inc, USA) is a product that fits these criteria. Here, we present data on the impact of a Bio-Mos supplemented diet on the mRNA copy number of the antimicrobial peptide dicentracin, whose transcript regulation has not yet been explored in fish.We analyzed Bio-Mos-induced changes in the expression of sea bass (Dicentrarchus labrax) dicentracin,using a one-tube two-temperature real-time RT-PCR with which the gene expression can be absolutely quantified using the standard curve method. Our results revealed that 30 days of feeding fish with diets containing Bio-Mos supplemented at either 3 per thousand or 5 per thousand significantly increased the dicentracin mRNA copy number in the head kidney. Furthermore, the mRNA copy number in fish fed at 3 per thousand was significantly higher than that of the group fed at 5 per thousand for the same period of feeding Bio-Mos. A longer feeding period (60 days)did not further increase the dicentracin transcript levels as compared to the values recorded after 30 days of feeding either in the group fed at 3 per thousand or in the one fed at 5 per thousand diet. However, the transcript levels in fish fed at 3 per thousand proved to be significantly higher than those of the controls after 60 days of feeding. These findings offer new information about the response of antimicrobial peptides at the transcriptional level to diets supplemented with immune response modulators, and support a role of Bio-Mos in promoting sea bass nonspecific immune system.
authors.affiliation: Department of Biotechnology and Molecular Sciences, University of Insubria, Via J.H. Dunant, 3-21100 Varese, Italy. genciana.terova@uninsubria.it
authors.forename: Genciana
authors.initials: G
authors.lastname: Terova
authors.forename: Andrea
authors.initials: A
authors.lastname: Forchino
authors.forename: Simona
authors.initials: S
authors.lastname: Rimoldi
authors.forename: Fabio
authors.initials: F
authors.lastname: Brambilla
authors.forename: Micaela
authors.initials: M
authors.lastname: Antonini
authors.forename: Marco
authors.initials: M
authors.lastname: Saroglia
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000008:administration & dosage*; D017756:Aquaculture / Q000379:methods; D001492:Bass / Q000235:genetics / Q000378:metabolism; D019587:Dietary Supplements; D029941:Fish Proteins / Q000235:genetics* / Q000378:metabolism; D005786:Gene Expression Regulation; D006801:Humans; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods
chemical_list: D000900:Anti-Bacterial Agents; D029941:Fish Proteins; C520532:dicentracin protein, Dicentrarchus labrax