Use of reverse transcriptase PCR to subtype N1 to N9 neuraminidase genes of avian influenza viruses.
Reverse transcriptase PCR designed to amplify the N1 to N9 neuraminidase (NA) genes of avian influenza viruses detected 118 of the 119 NA genes tested (99.2%) in a subtype-specific manner. This technique successfully subtyped all 167 recent avian influenza viruses isolated from birds. Subtype specificity was confirmed by sequence analyses of all 285 PCR products.
Tsukamoto
Kenji
K
Research Team for Zoonotic Diseases, National Institute of Animal Health, Tsukuba, Ibaraki, Japan. ktsukamo@affrc.go.jp
Ashizawa
Takayoshi
T
Nakanishi
Koji
K
Kaji
Noriyuki
N
Suzuki
Kotaro
K
Shishido
Makiko
M
Okamatsu
Masatoshi
M
Mase
Masaji
M
D000818:Animals; D001717:Birds; D009980:Influenza A virus / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D005585:Influenza in Birds / Q000821:virology*; D008969:Molecular Sequence Data; D009439:Neuraminidase / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D017422:Sequence Analysis, DNA; D014764:Viral Proteins / Q000235:genetics*
D014764:Viral Proteins; C487630:NA protein, influenza A virus; D009439:Neuraminidase