Column high-performance liquid chromatographic method for the simultaneous determination of rosiglitazone and metformin in a pharmaceutical preparation.
An efficient, sensitive, and simple method was developed for the simultaneous determination of rosiglitazone and metformin hydrochloride in a combination tablet dosage form by column high-performance liquid chromatography. The mobile phase used was ammonium dihydrogen phosphate adjusted to pH 5.25 with sodium hydroxide. The limits of detection and quantitation were in the range of 0.5-1.6 microg/mL, respectively, for metformin hydrochloride, and 0.00201-0.0067 microg/mL, respectively, for rosiglitazone. The linearity was studied in the concentration range of 0.12-0.31 microg/mL for rosiglitazone and 30.6-76.7 microg/mL for metformin hydrochloride. The recovered amounts of metformin hydrochloride and rosiglitazone were 100-103.8 and 101-103.7%, respectively.
Ali
Azzam R
AR
Research and Development, Mid Pharma, Amman, Jordan.
Duraidi
Ibrahim I
II
Saket
Munib M
MM
Abu-Nameh
Eyad S M
ES
D002851:Chromatography, High Pressure Liquid / Q000379:methods; D007004:Hypoglycemic Agents / Q000032:analysis; D013058:Mass Spectrometry / Q000379:methods; D008687:Metformin / Q000032:analysis*; D004364:Pharmaceutical Preparations / Q000032:analysis*; D010919:Placebos; D015203:Reproducibility of Results; D000077154:Rosiglitazone; D012680:Sensitivity and Specificity; D012996:Solutions; D013056:Spectrophotometry, Ultraviolet; D013696:Temperature; D045162:Thiazolidinediones / Q000032:analysis*
D007004:Hypoglycemic Agents; D004364:Pharmaceutical Preparations; D010919:Placebos; D012996:Solutions; D045162:Thiazolidinediones; D000077154:Rosiglitazone; D008687:Metformin