Systems biology coupled with label-free high-throughput detection as a novel approach for diagnosis of chronic obstructive pulmonary disease.
Chronic obstructive pulmonary disease (COPD) is a treatable and preventable disease state, characterised by progressive airflow limitation that is not fully reversible. Although COPD is primarily a disease of the lungs there is now an appreciation that many of the manifestations of disease are outside the lung, leading to the notion that COPD is a systemic disease. Currently, diagnosis of COPD relies on largely descriptive measures to enable classification, such as symptoms and lung function. Here the limitations of existing diagnostic strategies of COPD are discussed and systems biology approaches to diagnosis that build upon current molecular knowledge of the disease are described. These approaches rely on new 'label-free' sensing technologies, such as high-throughput surface plasmon resonance (SPR), that we also describe.
Richens
Joanna L
JL
Cell Biophysics Group, School of Biology, The University of Nottingham, NG7 2RD, UK. joanna.richens@nottingham.ac.uk
Urbanowicz
Richard A
RA
Lunt
Elizabeth A M
EA
Metcalf
Rebecca
R
Corne
Jonathan
J
Fairclough
Lucy
L
O'Shea
Paul
P
D000465:Algorithms; D015415:Biomarkers / Q000032:analysis; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D003936:Diagnosis, Computer-Assisted / Q000379:methods*; D020869:Gene Expression Profiling / Q000379:methods*; D006801:Humans; D020543:Proteome / Q000032:analysis*; D029424:Pulmonary Disease, Chronic Obstructive / Q000175:diagnosis* / Q000378:metabolism*; D013194:Staining and Labeling; D020349:Surface Plasmon Resonance / Q000295:instrumentation / Q000379:methods*; D049490:Systems Biology / Q000379:methods
D015415:Biomarkers; D020543:Proteome