Protective effect of 3,5,3'-triiodothyroacetic and 3,5,3',5'-tetraiodothyroacetic acids on serum albumin fibrillation.
Inhibition or reversion of protein self-aggregation has been suggested as a possible preventive mechanism against amyloid diseases, and many efforts are underway to found out molecules capable to restrain the protein aggregation process. In this paper, the inhibitory effects of thyroid hormone analogues on heat-induced fibrillation process of serum albumin are reported. Among the analogues tested, 3,5,3',5'-tetraiodothyroacetic and 3,5,3'-triiodothyroacetic acid showed the most important inhibitory effects on amyloid formation. Thyroxine exhibits a lesser protective effect, while 3,5,3'-triiodothyronine showed no significant inhibition. The gaining of a negative charge together with a size reduction of the hormone molecule could play an essential role in the inhibition of fibrils formation. According to infrared spectroscopy results, the thyroid hormones analogues protective effects proceed via the stabilization of the protein native structure. The current work demonstrates the effectiveness of naturally occurring molecules in the inhibition of albumin fibril formation.
Cortez
Leonardo M
LM
Departamento Bioquímica de la Nutrición, Instituto Superior de Investigaciones Biológicas, Consejo Nacional de Investigaciones Científicas y Tecnológicas, Universidad Nacional de Tucumán, Chacabuco 461, San Miguel de Tucumán, 4000, Argentina.
Farías
Ricardo N
RN
Chehín
Rosana N
RN
D000682:Amyloid / Q000378:metabolism; D000818:Animals; D002417:Cattle; D004305:Dose-Response Relationship, Drug; D015195:Drug Design; D006358:Hot Temperature; D007700:Kinetics; D011485:Protein Binding / Q000187:drug effects; D017433:Protein Structure, Secondary / Q000187:drug effects; D012709:Serum Albumin / Q000737:chemistry / Q000378:metabolism*; D017550:Spectroscopy, Fourier Transform Infrared; D013974:Thyroxine / Q000031:analogs & derivatives* / Q000494:pharmacology; D014284:Triiodothyronine / Q000031:analogs & derivatives* / Q000494:pharmacology
D000682:Amyloid; D012709:Serum Albumin; D014284:Triiodothyronine; C010642:3,3',5-triiodothyroacetic acid; C011126:tetraiodothyroacetic acid; D013974:Thyroxine