title: Analysis of genetic variability of Plasmodium vivax isolates from different Brazilian Amazon areas using tandem repeats.
abstract: Few genetic markers have been described to analyze populations of Plasmodium vivax. The genetic variability of P. vivax has been analyzed mainly among isolates taken from areas ranging from hyper- to holoendemic areas. These studies of genetic variability have neglected many areas with different epidemiologic profiles. The purpose of this study was to analyze the genetic variability of P. vivax isolates from four different Brazilian Amazon areas. We chose to study the five most polymorphic tandem repeats (TRs) identified so far. All TRs studied were polymorphic in at least one studied population, with a modal allele at nearly all loci. Expected heterozygosity ranged from 0.462 to 0.666 and did not correlate with the repeat array length. The genetic distances among the populations varied from 0.027 to 0.241, and did not correlate with their geographic separation. Tandem repeats identified in P. vivax isolates failed to allow geographic clustering.
authors.affiliation: Laboratory of Malaria, Centro de Pesquisas René Rachou/Fiocruz, Belo Horizonte, Brazil.
authors.forename: Antônio M
authors.initials: AM
authors.lastname: Rezende
authors.forename: Eduardo
authors.initials: E
authors.lastname: Tarazona-Santos
authors.forename: Alvaro D' Almeida
authors.initials: AD
authors.lastname: Couto
authors.forename: Cór Jésus F
authors.initials: CJ
authors.lastname: Fontes
authors.forename: José Maria
authors.initials: JM
authors.lastname: De Souza
authors.forename: Luzia H
authors.initials: LH
authors.lastname: Carvalho
authors.forename: Cristiana F A
authors.initials: CF
authors.lastname: Brito
mesh_terms: D000818:Animals; D005075:Biological Evolution; D001938:Brazil / Q000453:epidemiology; D016054:DNA, Protozoan / Q000235:genetics*; D005819:Genetic Markers; D014644:Genetic Variation; D006579:Heterozygote; D006801:Humans; D016780:Malaria, Vivax / Q000453:epidemiology / Q000469:parasitology*; D010966:Plasmodium vivax / Q000235:genetics*; D020080:Tandem Repeat Sequences / Q000235:genetics*
chemical_list: D016054:DNA, Protozoan; D005819:Genetic Markers