title: Plasma phospholipid metabolic profiling and biomarkers of rats following radiation exposure based on liquid chromatography-mass spectrometry technique.
abstract: Lipidomics, a prominent area of metabolomics, utilizes novel analytical methodologies to study the extensive classes of lipid molecules, changes in lipid metabolism and lipid-mediated signaling processes. In this paper, the phospholipid metabolic profiles changes and potential biomarker identification in the rats plasma after gamma-irradiation exposure were investigated by coupling high performance liquid chromatography-mass spectrometry technology to multivariate statistical analysis. Orthogonal partial least-squares to latent structures discriminate analysis (OPLS-DA) was used to find the potential plasma phospholipids biomarkers of rats for radiation exposure. According to the corresponding tandem mass spectrometric results, potential biomarkers were identified. After exposure to gamma-rays, phosphatidylethanolamine and phosphatidylserine showed a marked increase, and phosphatidylcholine, sphingomyelin and lysophosphatidylcholine followed the same trend, although their rise was not significant. The results suggested that radiated rats had a phospholipid metabolic abnormality, which could be an alternative way to assess the radiaton exposure. The biomarkers may involve a radiation-induced apoptosis pathway and represent a promising target for discovery new radioprotective drugs and radiosensitizers.
authors.affiliation: Jiangsu Provincial Key Laboratory of Radiation Medicine and Protection, School of Radiation Medicine and Public Health, Medical College of Soochow University, Suzhou 215123, People's Republic of China. wangchang@suda.edu.cn
authors.forename: Chang
authors.initials: C
authors.lastname: Wang
authors.forename: Jun
authors.initials: J
authors.lastname: Yang
authors.forename: Jihua
authors.initials: J
authors.lastname: Nie
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000097:blood / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005720:Gamma Rays; D016018:Least-Squares Analysis; D050356:Lipid Metabolism / Q000528:radiation effects*; D008297:Male; D013058:Mass Spectrometry / Q000379:methods*; D055432:Metabolomics / Q000379:methods; D015999:Multivariate Analysis; D010743:Phospholipids / Q000097:blood* / Q000737:chemistry; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D015415:Biomarkers; D010743:Phospholipids