title: Bovine colostral antibody against verotoxin 2 derived from Escherichia coli O157:H7: resistance to proteases and effects in beagle dogs.
abstract: A bovine colostral antibody against verotoxin (VT) 2 of Escherichia coli O157:H7 was administered orally to beagle dogs. The antibody remained in the dogs' small intestine for at least 2 h, whereas little serum antibody remained 1.5 h after administration. Furthermore, the antibody activity of secretory IgA did not change until 2 h after administration; however, the activity of IgG and IgM antibodies decreased by approximately 60% and 40% at 2 h after administration, respectively. Seven beagle dogs inoculated with Escherichia coli O157:H7 producing VT2 were administered bovine colostral antibody or bovine colostral whey without antibody. With administration of bovine colostral whey without antibody, the amount of VT2 in feces decreased gradually after administration and increased again at 5 d after inoculation, whereas bovine colostral antibody significantly reduced the amount of VT2 in feces on the day after administration. In addition, 9 beagle dogs were given bovine colostral antibody, bovine plasma antibody, or saline. The amount of VT2 in feces again decreased significantly more rapidly after administration of bovine colostral antibody than after administration of bovine plasma antibody or saline.
authors.affiliation: Laboratory of Immunology, School of Life and Environmental Science, Azabu University, Sagamihara, Kanagawa, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Kuribayashi
authors.forename: Tetsuro
authors.initials: T
authors.lastname: Seita
authors.forename: Mariko
authors.initials: M
authors.lastname: Matsumoto
authors.forename: Katsunori
authors.initials: K
authors.lastname: Furuhata
authors.forename: Kazutoshi
authors.initials: K
authors.lastname: Tagata
authors.forename: Shizuo
authors.initials: S
authors.lastname: Yamamoto
mesh_terms: D000818:Animals; D000906:Antibodies / Q000008:administration & dosage / Q000276:immunology*; D002417:Cattle; D003126:Colostrum / Q000737:chemistry / Q000276:immunology*; D004285:Dogs; D019453:Escherichia coli O157 / Q000276:immunology* / Q000472:pathogenicity; D005260:Female; D006801:Humans; D007136:Immunoglobulins / Q000276:immunology; D008297:Male; D010447:Peptide Hydrolases / Q000378:metabolism*; D011247:Pregnancy; D022641:Shiga Toxin 2 / Q000276:immunology*
chemical_list: D000906:Antibodies; D007136:Immunoglobulins; D022641:Shiga Toxin 2; D010447:Peptide Hydrolases