title: Cholinesterase inhibitory effects of the extracts and compounds of Maclura pomifera (Rafin.) Schneider.
abstract: We have investigated anticholinesterase potential of the methanol extracts from the leaf, wood, flower, twig, and stem bark of the female and male individuals and rhizodermis and fruit from the female tree of Maclura pomifera (Rafin.) Schneider (Moraceae) along with its major isoflavonoids; osajin and pomiferin as well as their semi-synthetic derivatives; iso-osajin and iso-pomiferin. Anticholinesterase activity was determined by Ellman method using ELISA microplate reader. Osajin and pomiferin had a noticeable inhibition of AChE with IC(50) values of 2.239 and 0.096 mM, respectively, while their iso-derivatives were found to display less inhibition towards AChE. The extracts and compounds did not inhibit BChE. The extracts were analyzed for osajin and pomiferin contents by LC-DAD-MS and only the fruits and female flowers contained osajin (fruit: 8.87%, female flowers: 0.19%, w/w) and pomiferin (fruit: 13.6%, female flowers: 0.36%, w/w).
authors.affiliation: Department of Pharmacognosy, Faculty of Pharmacy, Gazi University, 06330 Ankara, Turkey. iorhan@gazi.edu.tr
authors.forename: I
authors.initials: I
authors.lastname: Orhan
authors.forename: F S
authors.initials: FS
authors.lastname: Senol
authors.forename: M
authors.initials: M
authors.lastname: Kartal
authors.forename: M
authors.initials: M
authors.lastname: Dvorská
authors.forename: M
authors.initials: M
authors.lastname: Zemlicka
authors.forename: K
authors.initials: K
authors.lastname: Smejkal
authors.forename: P
authors.initials: P
authors.lastname: Mokrý
mesh_terms: D000818:Animals; D001578:Benzopyrans / Q000737:chemistry / Q000302:isolation & purification; D002091:Butyrylcholinesterase / Q000378:metabolism; D002800:Cholinesterase Inhibitors / Q000494:pharmacology; D002851:Chromatography, High Pressure Liquid; D004593:Electrophorus; D004797:Enzyme-Linked Immunosorbent Assay; D005702:Galantamine / Q000494:pharmacology; D007529:Isoflavones / Q000737:chemistry / Q000302:isolation & purification; D031624:Maclura / Q000737:chemistry*; D013058:Mass Spectrometry; D010936:Plant Extracts / Q000494:pharmacology
chemical_list: D001578:Benzopyrans; D002800:Cholinesterase Inhibitors; D007529:Isoflavones; D010936:Plant Extracts; D005702:Galantamine; C474837:pomiferin; C485800:osajin; D002091:Butyrylcholinesterase