title: Ultra low-dose radiation: stress responses and impacts using rice as a grass model.
abstract: We report molecular changes in leaves of rice plants (Oryza sativa L. - reference crop plant and grass model) exposed to ultra low-dose ionizing radiation, first using contaminated soil from the exclusion zone around Chernobyl reactor site. Results revealed induction of stress-related marker genes (Northern blot) and secondary metabolites (LC-MS/MS) in irradiated leaf segments over appropriate control. Second, employing the same in vitro model system, we replicated results of the first experiment using in-house fabricated sources of ultra low-dose gamma (gamma) rays and selected marker genes by RT-PCR. Results suggest the usefulness of the rice model in studying ultra low-dose radiation response/s.
authors.affiliation: Health Technology Research Center, National Institute of Advanced Industrial Science and Technology (AIST), West, Tsukuba, Ibaraki 305-8569, Japan.|Research Laboratory for Biotechnology and Biochemistry (RLABB), GPO Box 8207, Kathmandu, Nepal.
authors.forename: Randeep
authors.initials: R
authors.lastname: Rakwal
authors.affiliation: Research Laboratory for Biotechnology and Biochemistry (RLABB), GPO Box 8207, Kathmandu, Nepal.
authors.forename: Ganesh Kumar
authors.initials: GK
authors.lastname: Agrawal
authors.affiliation: Health Technology Research Center, National Institute of Advanced Industrial Science and Technology (AIST), West, Tsukuba, Ibaraki 305-8569, Japan.
authors.forename: Junko
authors.initials: J
authors.lastname: Shibato
authors.affiliation: Research Reactor Institute, Kyoto University (KURRI), Osaka, Japan.
authors.forename: Tetsuji
authors.initials: T
authors.lastname: Imanaka
authors.affiliation: Research Reactor Institute, Kyoto University (KURRI), Osaka, Japan.
authors.forename: Satoshi
authors.initials: S
authors.lastname: Fukutani
authors.affiliation: Laboratory of Growth Regulation Chemistry, Akita Prefectural University, Akita 010-0195, Japan.
authors.forename: Shigeru
authors.initials: S
authors.lastname: Tamogami
authors.affiliation: Graduate School of Engineering, Hiroshima University (HU), Hiroshima, Japan.
authors.forename: Satoru
authors.initials: S
authors.lastname: Endo
authors.affiliation: Research Center for Radiation Protection, National Institute of Radiological Sciences (NIRS), Chiba, Takoma Park, Maryland, USA.
authors.forename: Sarata Kumar Sahoo
authors.initials: SKS
authors.lastname: Sahoo
authors.affiliation: Health Technology Research Center, National Institute of Advanced Industrial Science and Technology (AIST), West, Tsukuba, Ibaraki 305-8569, Japan.
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Masuo
authors.affiliation: Department of Research Planning and Coordination, Japan NIOSH, Kawasaki, Japan.
authors.forename: Shinzo
authors.initials: S
authors.lastname: Kimura
mesh_terms: D005720:Gamma Rays; D008954:Models, Biological; D012275:Oryza / Q000378:metabolism / Q000528:radiation effects*; D018515:Plant Leaves / Q000378:metabolism / Q000528:radiation effects; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012717:Sesquiterpenes / Q000737:chemistry / Q000378:metabolism; D012987:Soil / Q000737:chemistry; D013312:Stress, Physiological / Q000528:radiation effects; D000094162:Phytoalexins
chemical_list: D010940:Plant Proteins; D012333:RNA, Messenger; D012717:Sesquiterpenes; D012987:Soil; D000094162:Phytoalexins