title: Nuclear relaxation and critical fluctuations in membranes containing cholesterol.
abstract: Nuclear resonance frequencies in bilayer membranes depend on lipid composition. Our calculations describe the combined effects of composition fluctuations and diffusion on nuclear relaxation near a miscibility critical point. Both tracer and gradient diffusion are included. The calculations involve correlation functions and a correlation length xi=xi(0)T/(T-T(c)), where T-T(c) is temperature above the critical temperature and xi(0) is a parameter of molecular length. Several correlation functions are examined, each of which is related in some degree to the Ising model correlation function. These correlation functions are used in the calculation of transverse deuterium relaxation rates in magic angle spinning and quadrupole echo experiments. The calculations are compared with experiments that report maxima in deuterium and proton nuclear relaxation rates at the critical temperature [Veatch et al., Proc. Nat. Acad. Sci. U.S.A. 104, 17650 (2007)]. One Ising-model-related correlation function yields a maximum 1/T(2) relaxation rate at the critical temperature for both magic angle spinning and quadrupole echo experiments. The calculated rates at the critical temperature are close to the experimental rates. The rate maxima involve relatively rapid tracer diffusion in a static composition gradient over distances of up to 10-100 nm.
authors.affiliation: Department of Chemistry, Stanford University, Stanford, California 94305, USA. harden@stanford.edu
authors.forename: Harden
authors.initials: H
authors.lastname: McConnell
mesh_terms: D015060:1,2-Dipalmitoylphosphatidylcholine / Q000737:chemistry; D002784:Cholesterol / Q000737:chemistry*; D003198:Computer Simulation; D003903:Deuterium / Q000032:analysis*; D004058:Diffusion; D008051:Lipid Bilayers / Q000737:chemistry*; D008954:Models, Biological; D009038:Motion; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods*; D013696:Temperature
chemical_list: D008051:Lipid Bilayers; D015060:1,2-Dipalmitoylphosphatidylcholine; D002784:Cholesterol; D003903:Deuterium