title: A new micro/nanoencapsulated porphyrin formulation for PDT treatment.
abstract: The highly hydrophobic 5,10,15-triphenyl-20-(3-N-methylpyridinium-yl)porphyrin (3MMe) cationic species was synthesized, characterized and encapsulated in marine atelocollagen/xanthane gum microcapsules by the coacervation method. Further reduction in the capsule size, from several microns down to about 300-400 nm, was carried out successfully by ultrasonic processing in the presence of up to 1.6% Tween 20 surfactant, without affecting the distribution of 3MMe in the oily core. The resulting cream-like product exhibited enhanced photodynamic activity but negligible cytotoxicity towards HeLa cells. The polymeric micro/nanocapsule formulation was found to be about 4 times more phototoxic than the respective phosphatidylcholine lipidic emulsion, demonstrating high potentiality for photodynamic therapy applications.
authors.affiliation: Institute of Chemistry, University of Sao Paulo, Sao Paulo, SP, Brazil.
authors.forename: Daiana K
authors.initials: DK
authors.lastname: Deda
authors.forename: Adjaci F
authors.initials: AF
authors.lastname: Uchoa
authors.forename: Eduardo
authors.initials: E
authors.lastname: Caritá
authors.forename: Maurício S
authors.initials: MS
authors.lastname: Baptista
authors.forename: Henrique E
authors.initials: HE
authors.lastname: Toma
authors.forename: Koiti
authors.initials: K
authors.lastname: Araki
mesh_terms: D002214:Capsules / Q000138:chemical synthesis / Q000737:chemistry*; D002470:Cell Survival / Q000187:drug effects; D004337:Drug Carriers / Q000138:chemical synthesis*; D016503:Drug Delivery Systems / Q000379:methods*; D006367:HeLa Cells; D006801:Humans; D053769:Nanocapsules / Q000737:chemistry*; D010778:Photochemotherapy / Q000379:methods*; D011166:Porphyrins / Q000008:administration & dosage* / Q000138:chemical synthesis / Q000494:pharmacology
chemical_list: D002214:Capsules; D004337:Drug Carriers; D053769:Nanocapsules; D011166:Porphyrins