Generate Western blot protein marker from a single construct.
An expression construct, consisting of a tandem arrangement of nucleic acids coding for the constant fragments (Fc) receptor of protein G combined with nucleic acids for the Fc receptor of protein A, was constructed. When the construct was expressed in Escherichia coli, proteins of estimated molecular weights of 25, 30, 50, 58, 80, and 85 kDa were consistently obtained from this expression construct due to possible proteolytic degradation during the cultivation and purification steps. The purified proteins from this single expression construct were used as Western blot protein marker.
Zhang
Yi-Ming
YM
Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China.
Geng
Liang
L
Huang
Da-Wei
DW
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000032:analysis* / Q000235:genetics / Q000302:isolation & purification; D015153:Blotting, Western / Q000379:methods*; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics; D008969:Molecular Sequence Data; D008970:Molecular Weight; D011961:Receptors, Fc / Q000032:analysis* / Q000235:genetics / Q000302:isolation & purification; D013205:Staphylococcal Protein A / Q000032:analysis* / Q000235:genetics / Q000302:isolation & purification
D001426:Bacterial Proteins; C034284:IgG Fc-binding protein, Streptococcus; D011961:Receptors, Fc; D013205:Staphylococcal Protein A