title: Morphologic organization of pulmonary granulomas in mice infected with Paracoccidioides brasiliensis.
abstract: A morphologic study of the lungs was carried out in Swiss mice infected with yeast isolated from Paracoccidioides brasiliensis (Pb18). The lung was processed 1, 2, 4, and 8 weeks after inoculation for histologic staining with hematoxylin and eosin (H&E), methenamine silver nitrate (Gomori-Grocott), and picrosirius to qualitative and quantitative analyses of the granulomas and the presence of fungal lesions. The numbers of CFUs/g counted in the lungs were 189.8 +/- 20.64, 353.6 +/- 46.21, 547.2 +/- 108.1, and 295.2 +/- 89.17 in the first, second, fourth, and eighth weeks, respectively. One week after infection, inflammatory cells and reticular and collagens fibers, the latest typical of fibrosis, were detected. After 2 and 4 weeks, a progressive intensification of the infection and fibrosis was observed, but in week 8 a more organized granuloma was evident, with macrophages, epithelioid cells, and yeasts in the central portion, and intense peripheral basophilia. Pycnotic structures typical of apoptotic bodies were observed in weeks 1 and 8. The different histologic staining used acted as a fundamental tool for the study of the morphologic organization of granuloma formation.
authors.affiliation: Post-graduate Program in Clinical Analyses, Laboratory of Medical Mycology, Universidade Estadual de Maringá, Maringá, Paraná, Brazil. nandacristie@gmail.com
authors.forename: Fernanda C
authors.initials: FC
authors.lastname: Da Silva
authors.forename: Terezinha I E
authors.initials: TI
authors.lastname: Svidzinski
authors.forename: Eliana V
authors.initials: EV
authors.lastname: Patussi
authors.forename: Cristina P
authors.initials: CP
authors.lastname: Cardoso
authors.forename: Márcia M
authors.initials: MM
authors.lastname: De Oliveira Dalalio
authors.forename: Luzmarina
authors.initials: L
authors.lastname: Hernandes
mesh_terms: D000818:Animals; D006099:Granuloma / Q000382:microbiology / Q000473:pathology*; D008168:Lung / Q000382:microbiology / Q000473:pathology; D008172:Lung Diseases, Fungal / Q000382:microbiology / Q000473:pathology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D010228:Paracoccidioides / Q000302:isolation & purification; D010229:Paracoccidioidomycosis / Q000382:microbiology / Q000473:pathology*