title: Mycobacterium riyadhense sp. nov., a non-tuberculous species identified as Mycobacterium tuberculosis complex by a commercial line-probe assay.
abstract: A non-chromogenic, slowly growing Mycobacterium strain was isolated from a maxillary sinus lavage from a symptomatic patient in Riyadh, Saudi Arabia. It was initially identified as a member of the Mycobacterium tuberculosis complex by a commercial line-probe assay. Its 16S rRNA, hsp65 and rpoB gene and 16S-23S internal transcribed spacer sequences were unique; phylogenetic analysis based on the 16S rRNA gene sequence groups this organism close to Mycobacterium szulgai and Mycobacterium malmoense. Its unique biochemical properties and mycolic acid profile support separate species status. We propose the name Mycobacterium riyadhense sp. nov. to accommodate this strain. The type strain is NLA000201958(T) (=CIP 109808(T) =DSM 45176(T)).
authors.affiliation: National Mycobacteria Reference Laboratory, National Institute for Public Health and the Environment, Bilthoven, The Netherlands. jakko.van.ingen@rivm.nl
authors.forename: Jakko
authors.initials: J
authors.lastname: van Ingen
authors.forename: Sahal A M
authors.initials: SA
authors.lastname: Al-Hajoj
authors.forename: Martin
authors.initials: M
authors.lastname: Boeree
authors.forename: Fahad
authors.initials: F
authors.lastname: Al-Rabiah
authors.forename: Mimount
authors.initials: M
authors.lastname: Enaimi
authors.forename: Rina
authors.initials: R
authors.lastname: de Zwaan
authors.forename: Enrico
authors.initials: E
authors.lastname: Tortoli
authors.forename: Richard
authors.initials: R
authors.lastname: Dekhuijzen
authors.forename: Dick
authors.initials: D
authors.lastname: van Soolingen
mesh_terms: D000328:Adult; D001426:Bacterial Proteins / Q000235:genetics; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000235:genetics; D003951:Diagnostic Errors; D020459:Genes, rRNA; D006801:Humans; D008297:Male; D008443:Maxillary Sinus / Q000382:microbiology*; D015523:Maxillary Sinusitis / Q000175:diagnosis / Q000382:microbiology; D015336:Molecular Probe Techniques; D008969:Molecular Sequence Data; D009161:Mycobacterium / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology; D009164:Mycobacterium Infections / Q000175:diagnosis / Q000382:microbiology; D009169:Mycobacterium tuberculosis / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D009171:Mycolic Acids / Q000032:analysis; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012338:RNA, Ribosomal, 23S / Q000235:genetics; D011933:Reagent Kits, Diagnostic; D012529:Saudi Arabia; D017422:Sequence Analysis, DNA; D013045:Species Specificity; D007507:Therapeutic Irrigation; D014376:Tuberculosis / Q000175:diagnosis / Q000382:microbiology; D055815:Young Adult
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D021903:DNA, Ribosomal Spacer; D009171:Mycolic Acids; D012336:RNA, Ribosomal, 16S; D012338:RNA, Ribosomal, 23S; D011933:Reagent Kits, Diagnostic