In vivo recognition of Bacillus subtilis by desorption electrospray ionization mass spectrometry (DESI-MS).
Desorption electrospray ionization mass spectrometry (DESI-MS) of culture of the bacterium Bacillus subtilis as a biofilm growing on agar nutrient gives simple, high quality mass spectra dominated in both the positive and negative ion modes by signals due to the cyclic lipopeptide, Surfactin(C15). This in vivo experiment, performed by direct analysis of untreated microorganism samples under ambient conditions, allows rapid identification of this microorganism and the antibiotics that it produces. The result is suggestive of the capabilities of DESI-MS for in vivo microorganism characterization in general and for monitoring fermentation processes for the production of antibiotics and other biochemicals.
Song
Yishu
Y
Department of Chemistry, Purdue University, West Lafayette, IN 47907, USA.
Talaty
Nari
N
Datsenko
Kirill
K
Wanner
Barry L
BL
Cooks
R Graham
RG
D001412:Bacillus subtilis / Q000302:isolation & purification*; D001431:Bacteriological Techniques; D018441:Biofilms; D055666:Lipopeptides / Q000737:chemistry; D010456:Peptides, Cyclic / Q000737:chemistry; D011487:Protein Conformation; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
D055666:Lipopeptides; D010456:Peptides, Cyclic; C009365:surfactin peptide