title: Expression of metallothionein mRNAs on mouse cerebellum microglia cells by thimerosal and its metabolites.
abstract: Effects of thimerosal and its metabolites, ethyl mercury and thiosalicylate, on the expression of metallothionein (MT) mRNAs in mouse cerebellum microglia cell line, C8-B4 cells, were studied. The level of MT-1 mRNA significantly decreased at early hours and recovered time-dependently 24h after thimerosal was added to the C8-B4 cells. However, MT-2 and MT-3 mRNA expressions did not change from the control group. In contrast, the expression of MT-1 mRNA increased in a mouse neuroblastoma cell line 6h after incubation with thimerosal. In addition, the level of MT-1 mRNA decreased in C8-B4 cells 6h after the addition of thiosalicylate, but ethyl mercury induced MT-1 mRNA expression. When cell viability was compared with thimerosal, thiosalicylate, and ethyl mercury, the viability of C8-B4 cells decreased dose-dependently 24h after either thimerosal or ethyl mercury was added; however, the viability increased dose-dependently until 15 microM thiosalicylate was added. From the present results, it is concluded that the expression of MT-1 mRNA may be mediated by different factors than the expression of MT-2 mRNA in C8-B4 cells. The reduction of MT-1 mRNA level by thiosalicylate may affect the proliferation of C8-B4 cells.
authors.affiliation: Department of Life Sciences, School of Science & Engineering, Kinki University, 3-4-1 Kowakae, Higashi-osaka, Osaka 577-8502, Japan. minamita@life.kindai.ac.jp
authors.forename: Takeshi
authors.initials: T
authors.lastname: Minami
authors.forename: Eriko
authors.initials: E
authors.lastname: Miyata
authors.forename: Yamato
authors.initials: Y
authors.lastname: Sakamoto
authors.forename: Azusa
authors.initials: A
authors.lastname: Kohama
authors.forename: Hideo
authors.initials: H
authors.lastname: Yamazaki
authors.forename: Seiji
authors.initials: S
authors.lastname: Ichida
mesh_terms: D000818:Animals; D001711:Biotransformation; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D002531:Cerebellum / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D004305:Dose-Response Relationship, Drug; D005786:Gene Expression Regulation / Q000187:drug effects; D005904:Glial Fibrillary Acidic Protein; D008668:Metallothionein / Q000235:genetics*; D000090023:Metallothionein 3; D051379:Mice; D017628:Microglia / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D009419:Nerve Tissue Proteins / Q000235:genetics; D011310:Preservatives, Pharmaceutical / Q000378:metabolism / Q000633:toxicity*; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats; D012459:Salicylates / Q000378:metabolism / Q000633:toxicity*; D013438:Sulfhydryl Compounds / Q000378:metabolism / Q000633:toxicity*; D013849:Thimerosal / Q000378:metabolism / Q000633:toxicity*; D013997:Time Factors; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics; D000509:alpha-Fetoproteins / Q000235:genetics
chemical_list: D005904:Glial Fibrillary Acidic Protein; D000090023:Metallothionein 3; C491054:Mt2 protein, mouse; C000715430:Mt3 protein, mouse; D009419:Nerve Tissue Proteins; D011310:Preservatives, Pharmaceutical; D012333:RNA, Messenger; D012459:Salicylates; D013438:Sulfhydryl Compounds; D042461:Vascular Endothelial Growth Factor A; D000509:alpha-Fetoproteins; C528900:glial fibrillary astrocytic protein, mouse; C525355:metallothionein-1, mouse; C467480:vascular endothelial growth factor A, mouse; D013849:Thimerosal; D008668:Metallothionein; C441201:thiosalicylic acid