List-decoding methods for inferring polynomials in finite dynamical gene network models.
BACKGROUND
The problem of reverse engineering the dynamics of gene expression profiles is of focal importance in systems biology. Due to noise and the inherent lack of sufficiently large datasets generated via high-throughput measurements, known reconstruction frameworks based on dynamical systems models fail to provide adequate settings for network analysis. This motivates the study of new approaches that produce stochastic lists of explanations for the observed network dynamics that can be efficiently inferred from small sample sets and in the presence of errors.


RESULTS
We introduce a novel algebraic modeling framework, termed stochastic polynomial dynamical systems (SPDSs) that can capture the dynamics of regulatory networks based on microarray expression data. Here, we refer to dynamics of the network as the trajectories of gene expression profiles over time. The model assumes that the expression data is quantized in a manner that allows for imposing a finite field structure on the observations, and the existence of polynomial update functions for each gene in the network. The underlying reverse engineering algorithm is based on ideas borrowed from coding theory, and in particular, list-decoding methods for so called Reed-Muller codes. The list-decoding method was tested on synthetic data and on microarray expression measurements from the M(3D) database, corresponding to a subnetwork of the Escherichia coli SOS repair system, as well as on the complete transcription factor network, available at RegulonDB. The results show that SPDSs constructed via list-decoders significantly outperform other algebraic reverse engineering methods, and that they also provide good guidelines for estimating the influence of genes on the dynamics of the network.


BACKGROUND
Software codes for list-decoding algorithms suitable for direct application to quantized expression data will be publicly available at the authors' web-pages.
Dingel
Janis
J
Institute for Communications Engineering, Technische Universität München, Munich, Germany. janis.dingel@tum.de
Milenkovic
Olgica
O
D019295:Computational Biology / Q000379:methods*; D020869:Gene Expression Profiling / Q000379:methods; D053263:Gene Regulatory Networks; D008957:Models, Genetic; D049490:Systems Biology