Determination of matrix metalloproteinases in human radicular dentin.
Matrix metalloproteinases (MMPs) are present in sound coronal dentin and may play a role in collagen network degradation in bonded restorations. We investigated whether these enzymes can also be detected in root dentin. Crown and root sections of human teeth were powderized, and dentin proteins were extracted by using guanidine-HCl and EDTA. Extracts were analyzed by zymography and Western blotting for matrix metalloproteinases detection. Zymography revealed gelatinolytic activities in both crown and root dentin samples, corresponding to MMP-2 and MMP-9. MMP-2 was more evident in demineralized root dentin matrix, whereas MMP-9 was mostly extracted from the mineralized compartment of dentin and presented overall lower levels. Western blot analysis detected MMP-8 equally distributed in crown and root dentin. Because MMPs are also present in radicular dentin, their contribution to the degradation of resin-dentin bonds should be addressed in the development of restorative strategies for the root substrate.
Santos
Juliana
J
Department of Restorative Dentistry, Endodontics Area, Piracicaba Dental School, University of Campinas, Piracicaba, SP, Brazil.
Carrilho
Marcela
M
Tervahartiala
Taina
T
Sorsa
Timo
T
Breschi
Lorenzo
L
Mazzoni
Annalisa
A
Pashley
David
D
Tay
Franklin
F
Ferraz
Caio
C
Tjäderhane
Leo
L
D000328:Adult; D015153:Blotting, Western; D004396:Coloring Agents; D003804:Dentin / Q000201:enzymology*; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D020778:Matrix Metalloproteinase 2 / Q000032:analysis; D020784:Matrix Metalloproteinase 8 / Q000032:analysis; D020780:Matrix Metalloproteinase 9 / Q000032:analysis; D020782:Matrix Metalloproteinases / Q000032:analysis*; D008970:Molecular Weight; D019228:Tooth Crown / Q000201:enzymology; D017001:Tooth Demineralization / Q000201:enzymology; D014092:Tooth Root / Q000201:enzymology*; D055815:Young Adult
D004396:Coloring Agents; D020782:Matrix Metalloproteinases; C522361:MMP2 protein, human; D020778:Matrix Metalloproteinase 2; C488762:MMP8 protein, human; D020784:Matrix Metalloproteinase 8; D020780:Matrix Metalloproteinase 9