title: Clostridium sufflavum sp. nov., isolated from a methanogenic reactor treating cattle waste.
abstract: A strictly anaerobic, mesophilic, cellulolytic bacterial strain, designated CDT-1(T), was isolated from rice-straw residue from a methanogenic reactor treating waste from cattle farms. The isolation was performed using enrichment culture with filter paper as a substrate. Cells stained Gram-negative, but reacted Gram-positively in the KOH test. Cells were slightly curved rods and were motile by means of peritrichous flagella. The strain produced yellow pigment when grown on filter-paper fragments. Although spore formation was not confirmed microscopically, thermotolerant cells were produced when the strain was grown on filter paper. The optimum temperature for growth was 33 degrees C and the optimum pH was 7.4. Oxidase, catalase and nitrate-reducing activities were absent. The strain utilized xylose, fructose, glucose, cellobiose, xylooligosaccharide, cellulose (filter-paper fragments and ball-milled filter paper) and xylan. The major fermentation products were acetate, ethanol, H(2) and CO(2). The major cellular fatty acids were iso-C(15 : 0), iso-C(14 : 0) and C(16 : 0) DMA. The cell-wall peptidoglycan contained meso-diaminopimelic acid as the diagnostic diamino acid. The genomic DNA G+C content was 40.7 mol%. On the basis of 16S rRNA gene sequence similarities, strain CDT-1(T) could be placed in cluster III of the genus Clostridium, being closely related to type strains of Clostridium hungatei (96.6 % sequence similarity), Clostridium termitidis (96.2 %) and Clostridium papyrosolvens (96.1 %). On the basis of the cellular, physiological and phylogenetic differences between CDT-1(T) and its close relatives, this strain represents a novel species of the genus Clostridium, for which the name Clostridium sufflavum sp. nov. is proposed. The type strain is CDT-1(T) (=JCM 14807(T)=DSM 19573(T)).
authors.affiliation: Faculty of Agriculture, Yamagata University, Wakaba-machi 1-23, Tsuruoka, Yamagata 997-8555, Japan.
authors.forename: Tomomi
authors.initials: T
authors.lastname: Nishiyama
authors.forename: Atsuko
authors.initials: A
authors.lastname: Ueki
authors.forename: Nobuo
authors.initials: N
authors.lastname: Kaku
authors.forename: Katsuji
authors.initials: K
authors.lastname: Ueki
mesh_terms: D000693:Anaerobiosis; D000822:Animal Husbandry / Q000379:methods*; D000818:Animals; D015373:Bacterial Typing Techniques; D001482:Base Composition; D019149:Bioreactors; D002417:Cattle; D003013:Clostridium / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology; D005227:Fatty Acids / Q000032:analysis; D020459:Genes, rRNA; D007564:Japan; D008697:Methane / Q000378:metabolism*; D008969:Molecular Sequence Data; D012275:Oryza / Q000382:microbiology*; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012037:Refuse Disposal / Q000379:methods*; D017422:Sequence Analysis, DNA; D013045:Species Specificity
chemical_list: D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D008697:Methane