Analysis of the V1V2 region of the SIV envelope in the brains of morphine-dependent and control SIV/SHIV-infected macaques.
Six morphine-dependent and three control macaques were infected with a mixture of SIV/SHIV. Half of the animals in the morphine group developed accelerated disease (rapid progressor) and died within 20 weeks postinfection. The evolution of the envelope gene in the brain of the rapid progressor and morphine-dependent group along with that in the control group was assessed. Six to 10 clones from the brain of each macaque were sequenced and were compared against each other as well as against a challenge virus. Analysis of the sequences revealed that the diversity and divergence of the clones were higher in the control group as compared to the morphine-dependent macaques, although this difference was not statistically significant.
Rivera-Amill
Vanessa
V
Department of Microbiology, Ponce School of Medicine, Ponce, Puerto Rico 00732.
Noel
Richard J
RJ
Román
Ivelisse Rivera
IR
Flores
Yashira García
YG
Buch
Shilpa
S
Kumar
Anil
A
D000595:Amino Acid Sequence; D000818:Animals; D001921:Brain / Q000821:virology*; D008251:Macaca; D008969:Molecular Sequence Data; D009021:Morphine Dependence / Q000150:complications*; D011110:Polymorphism, Genetic; D012191:Retroviridae Proteins / Q000235:genetics*; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D016097:Simian Acquired Immunodeficiency Syndrome / Q000150:complications* / Q000821:virology*; D015302:Simian Immunodeficiency Virus / Q000235:genetics* / Q000302:isolation & purification; D014759:Viral Envelope Proteins / Q000235:genetics*
D012191:Retroviridae Proteins; C080347:SIV envelope protein gp110; D014759:Viral Envelope Proteins