title: Repeated intake of broccoli does not lead to higher plasma levels of sulforaphane in human volunteers.
abstract: The plasma pharmacokinetic characteristics of the chemopreventive isothiocyanate sulforaphane were determined in six human volunteers following single and repeated intake of raw broccoli. Initially, an analytical method utilising LC-MS/MS, capable of determining low levels of sulforaphane in human plasma was developed and validated. The plasma profile of the isothiocyanate best fitted a two-compartment pharmacokinetic model. Sulforaphane was rapidly absorbed with peak plasma levels being attained within 1.5h, and was characterised by a long terminal elimination phase. Repeated intake of broccoli had no impact on the pharmacokinetic behaviour or plasma levels of sulforaphane, and there was no evidence of accumulation.
authors.affiliation: Molecular Toxicology Group, University of Surrey, Guildford, Surrey GU2 7XH, UK.
authors.forename: Natalya
authors.initials: N
authors.lastname: Hanlon
authors.forename: Nick
authors.initials: N
authors.lastname: Coldham
authors.forename: Adriana
authors.initials: A
authors.lastname: Gielbert
authors.forename: Maurice J
authors.initials: MJ
authors.lastname: Sauer
authors.forename: Costas
authors.initials: C
authors.lastname: Ioannides
mesh_terms: D000293:Adolescent; D000328:Adult; D016588:Anticarcinogenic Agents / Q000097:blood*; D001937:Brassica / Q000737:chemistry*; D002853:Chromatography, Liquid; D005260:Female; D006801:Humans; D017879:Isothiocyanates; D008297:Male; D008875:Middle Aged; D013454:Sulfoxides; D053719:Tandem Mass Spectrometry; D013861:Thiocyanates / Q000097:blood*; D055815:Young Adult
chemical_list: D016588:Anticarcinogenic Agents; D017879:Isothiocyanates; D013454:Sulfoxides; D013861:Thiocyanates; C016766:sulforaphane