Phenotype and chondrogenic differentiation of mesenchymal cells from adipose tissue of different species.
Mesenchymal stem cells (MSCs) are multipotent cells capable of differentiating into several mesoderm lineages. They have been isolated from different tissues, such as bone marrow, adult peripheral blood, umbilical cord blood, and adipose tissue. The aim of this study was to analyze the differences in proliferation and phenotype of adipose tissue-derived MSCs from three different species, and to evaluate their capacity to differentiate into chondrocytes in vitro. A comparative study of cultured human, rabbit, and sheep mesenchymal cells from adipose tissue was carried out, and the main morphological parameters, proliferative activity, and expression of surface markers were characterized. Proliferation and flow cytometry data showed species-related differences between animal and human MSCs. Histological staining suggested that rabbit and sheep mesenchymal cells were able to differentiate into chondrocytic lineages. Human mesenchymal cells, though they could also differentiate, accomplished it with more difficulty than animal MSCs. These results could help to explain the differences in the chondrogenic capacity of sheep and rabbit MSCs when they are used as animal models compared to human mesenchymal cells in a clinical assay.
Martínez-Lorenzo
María José
MJ
Banco de Sangre y Tejidos de Aragón, Area de Tejidos, Zaragoza, Spain. mmartinezlor@salud.aragon.es
Royo-Cañas
María
M
Alegre-Aguarón
Elena
E
Desportes
Paula
P
Castiella
Tomás
T
García-Alvarez
Felícito
F
Larrad
Luis
L
D000273:Adipose Tissue / Q000166:cytology; D000818:Animals; D015703:Antigens, CD / Q000096:biosynthesis; D002454:Cell Differentiation; D019070:Cell Lineage; D002478:Cells, Cultured; D019902:Chondrocytes / Q000166:cytology*; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D010641:Phenotype; D011817:Rabbits; D012756:Sheep
D015703:Antigens, CD