title: Anti-CD(45)RB monoclonal antibody induces immunologic toleration by suppressing dendritic cells.
abstract: BACKGROUND
  In transplantation, the antigen presentation step of T cell activation determines whether the T cell rejects the graft, ignores the graft, or inhibits other T cells from attacking the graft. Dendritic cells (DCs) are the most potent antigen presenting cells that are capable of activating naïve T cells. The purpose of the present study was to investigate the influence of anti-CD(45)RB monoclonal antibody on the phenotypes and functions of DCs and to further illustrate the mechanism of anti-CD(45)RB monoclonal antibody-inducing immunologic tolerance.
  
  
  METHODS
  DCs from mice were cultured and treated with varying doses of anti-CD(45)RB monoclonal antibody at specific time points. Fluorescence-activated cell sorting (FACS) was used to verify the changes of phenotypes in the cultured DCs labeled with fluorescent antibody. DCs were also used as stimulators in mixed leukocyte reaction to detect their ability to stimulate T cell proliferation.
  
  
  RESULTS
  Anti-CD(45)RB monoclonal antibody induced a dose- and time-dependent suppression of maturation of DCs and a dose-dependent suppression of T cell proliferation in mixed leukocyte reaction when anti-CD(45)RB monoclonal antibody-treated DCs were used as stimulators.
  
  
  CONCLUSIONS
  One of the mechanisms of anti-CD(45)RB monoclonal antibody-induced tolerance was inhibiting maturation and function of DCs and inducing tolerogenic DCs to achieve a new immune balance.
authors.affiliation: Department of Ophthalmology, 3rd Affiliated Hospital, Sun Yat-sen University, Guangzhou, China.
authors.forename: Xiaoping
authors.initials: X
authors.lastname: Xia
authors.forename: Xiao
authors.initials: X
authors.lastname: Zhang
authors.forename: Xuyan
authors.initials: X
authors.lastname: Huang
authors.forename: Thomas E
authors.initials: TE
authors.lastname: Ichim
authors.forename: Mu
authors.initials: M
authors.lastname: Li
authors.forename: Patrick
authors.initials: P
authors.lastname: Luke
authors.forename: Weiping
authors.initials: W
authors.lastname: Min
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000008:administration & dosage / Q000276:immunology*; D017951:Antigen Presentation; D003713:Dendritic Cells / Q000276:immunology*; D007108:Immune Tolerance; D007155:Immunologic Factors / Q000008:administration & dosage / Q000276:immunology*; D007165:Immunosuppression Therapy / Q000379:methods*; D017493:Leukocyte Common Antigens / Q000037:antagonists & inhibitors* / Q000276:immunology; D007959:Lymphocyte Culture Test, Mixed; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL
chemical_list: D000911:Antibodies, Monoclonal; D007155:Immunologic Factors; D017493:Leukocyte Common Antigens