[Influence of temperature on the calibration curves in IRMA for neuron-specific enolase and its physicochemical interpretation].
BACKGROUND
immunoradiometric assay (IRMA) is one of the principal methods used for the analytical determination of neuron specific enolase (NSE) concentration. We studied the influence of temperature on the calibration curves obtained by this method, and a physicochemical justification based on two theoretical models is proposed.


METHODS
we used a commercially available RIA kit for NSE and a gamma counter. Data was analysed using Statistical software.


CONCLUSIONS
activity bound to the antibody increases with temperature, producing results that are consistent with two modifications to the four parameter and Langmuir equations.


CONCLUSIONS
the two models used successfully reproduce the results, with the adsorption model being preferable due to its greater simplicity and clearer physical significance.
Peris Conejero
T
T
Servicio de Medicina Nuclear, Unidad de Radiofarmacia, Hospital Clínico Universitario de Valencia, and Departamento de Química Física, Facultad de Farmacia, Universidad de Valencia, Valencia, Spain.
Olivares Pallerols
R
R
Moreno Frigols
J L
JL
D000327:Adsorption; D000465:Algorithms; D000937:Antigen-Antibody Reactions; D014408:Biomarkers, Tumor / Q000097:blood; D002138:Calibration; D002627:Chemistry, Physical; D006801:Humans; D015592:Immunoradiometric Assay / Q000592:standards*; D008956:Models, Chemical; D010751:Phosphopyruvate Hydratase / Q000097:blood*; D011933:Reagent Kits, Diagnostic; D012015:Reference Standards; D013696:Temperature
D014408:Biomarkers, Tumor; D011933:Reagent Kits, Diagnostic; D010751:Phosphopyruvate Hydratase