[Study on the determination method of the astragaloside IV in health food].
OBJECTIVE
To built an analytical methods to test Astragaloside NV in health food.


METHODS
The reaction conditions of ultrasonic extraction, liquid-liquid extraction, macroporousresin column chromatography, solid-phase extraction, derivatization and HPLC method were analyzed.


RESULTS
The sample preparations were set up as followed: The samples were extracted with methanol. The extraction solutions were liquid-liquid extracted by ether, water-saturation n-butyl alcohol and 1% KOH. The extraction solutions were further purified by DM-301 macroporousresin column chromatography and Oasis HLB 3cc extraction catridge in turn. The purified solutions were derived with chloroform and benzoyl chloride. The linear ranges were 3.84 - 153.60 microg/ml. The detection limits were 2.13 microg/ml. The recoveries were 74.85% - 91.83%.


CONCLUSIONS
The method was applied to detect the concentration of Astragaloside IV in health food. It was enough to wipe out impurity of samples. Detection limit and recycle ratio were satisfied.
Zhu
Lei
L
National Institute of Nutrition and Food Safety, China CDC, Beijing 100021, China. zhulei513@163.com
Wang
Zhutian
Z
Yang
Dajin
D
D002851:Chromatography, High Pressure Liquid / Q000379:methods; D019587:Dietary Supplements / Q000032:analysis*; D058870:Food, Organic / Q000032:analysis*; D012503:Saponins / Q000032:analysis*; D052616:Solid Phase Extraction / Q000379:methods; D014315:Triterpenes / Q000032:analysis*
D012503:Saponins; D014315:Triterpenes; C052064:astragaloside A