Detection of oxidized methionine in selected proteins, cellular extracts and  blood serums by novel anti-methionine sulfoxide antibodies.
Methionine sulfoxide (MetO) is a common posttranslational modification to proteins occurring in vivo.These modifications are prevalent when reactive oxygen species levels are increased. To enable the detection of MetO in pure and extracted proteins from various sources, we have developed novel antibodies that can recognize MetO-proteins. These antibodies are polyclonal antibodies raised against an oxidized methionine-rich zein protein (MetO-DZS18) that are shown to recognize methionine oxidation in pure proteins and mouse and yeast extracts. Furthermore, mouse serum albumin and immunoglobulin (IgG)were shown to accumulate MetO as function of age especially in serums of methionine sulfoxide reductase A knockout mice. Interestingly, high levels of methionine-oxidized IgG in serums of subjects diagnosed with Alzheimer's disease were detected by western blot analysis using these antibodies. It is suggested that anti-MetO-DZS18 antibodies can be applied in the identification of proteins that undergo methionine oxidation under oxidative stress, aging, or disease state conditions.
Oien
Derek B
DB
Department of Pharmacology and Toxicology, School of Pharmacy, University of Kansas, Lawrence, KS 66045, USA.
Canello
Tamar
T
Gabizon
Ruth
R
Gasset
Maria
M
Lundquist
Brandi L
BL
Burns
Jeff M
JM
Moskovitz
Jackob
J
D000375:Aging; D000818:Animals; D000906:Antibodies / Q000276:immunology*; D000918:Antibody Specificity; D001798:Blood Proteins / Q000737:chemistry; D002457:Cell Extracts / Q000737:chemistry*; D006801:Humans; D008715:Methionine / Q000031:analogs & derivatives* / Q000032:analysis / Q000097:blood / Q000276:immunology / Q000378:metabolism; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D011328:Prions / Q000737:chemistry; D011506:Proteins / Q000737:chemistry* / Q000276:immunology; D044967:Serum / Q000737:chemistry*
D000906:Antibodies; D001798:Blood Proteins; D002457:Cell Extracts; D011328:Prions; D011506:Proteins; D008715:Methionine; C013111:methionine sulfoxide