[The analyze of capability of biofilm synthesis by Staphylococcus aureus strains isolated from children with cystic fibrosis].
The aim of this study was to analyse the capability of biofilm synthesis by S. aureus isolates obtained from the respiratory tract of CF (cystic fibrosis) patients. A total of 297 S. aureus strains isolated from 33 CF patients, and 40 isolates obtained from healthy control children, were analysed. Extracellular slime production was determined using phenotypical methods (Congo red agar, and crystal violet) and molecular techniques (icaA and icaD genes amplification). All S. aureus strains possessed the icaA and icaD genes belonging to the operon responsible for slime synthesis. The isolates obtained from the respiratory tract of CS patients more frequently showed the ability to produce a biofilm than those from healthy individuals.
Semczuk
Katarzyna
K
Zakład Mikrobiologii i Immunologii Klinicznej, Instytut Pomnik Centrum Zdrowia Dziecka Warszawa.
Dzierzanowska-Fangrat
Katarzyna
K
Dmeńska
Hanna
H
Dzierzanowska
Danuta
D
D018441:Biofilms; D002648:Child; D003550:Cystic Fibrosis / Q000382:microbiology*; D006801:Humans; D009876:Operon; D010641:Phenotype; D012137:Respiratory System / Q000382:microbiology*; D013211:Staphylococcus aureus / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*