Purification, crystallization and preliminary X-ray diffraction analysis of a plant subtilase.
The subtilase SBT3 from Solanum lycopersicum (tomato) was purified from a tomato cell culture and crystallized using the sitting-drop vapour-diffusion method. A native data set was collected to 2.5 A resolution at 100 K using synchrotron radiation. For experimental phasing, CsCl-derivative and tetrakis(acetoxymercuri)methane (TAMM) derivative crystals were employed for MIRAS phasing. Three caesium sites and one TAMM site were identified, which allowed solution of the structure.
Rose
Rolf
R
Chemical Genomics Centre, Dortmund, Germany.
Huttenlocher
Franziska
F
Cedzich
Anna
A
Kaiser
Markus
M
Schaller
Andreas
A
Ottmann
Christian
C
D003460:Crystallization; D018360:Crystallography, X-Ray; D018551:Solanum lycopersicum / Q000201:enzymology* / Q000235:genetics; D013381:Subtilisins / Q000032:analysis* / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism
D013381:Subtilisins; C119297:subtilase, plant