title: Stabilization effects of kosmotrope systems on ornithine carbamoyltransferase.
abstract: In the present article the influence of salts and additives, such as trehalose, NaCl, ornithine, sodium phosphate and ammonium sulphate, on ornithine carbamoyltransferase (OCTase) is investigated in order to study the OCTase stabilization process as a function of solutes and to point out the fundamental role played by an enhancement of hydrophobic interactions. The synergic use of different techniques, such as neutron spectroscopy, UV-vis spectroscopy, activity and thermal measurements, allows to highlight the cosolute capability to avoid thermal inactivation, to induce important changes in secondary and tertiary enzyme structure and to stabilize biological macromolecules.
authors.affiliation: Dipartimento di Chimica Organica e Biologica, Università di Messina, P.O. Box 55, S.ta Sperone C.da Papardo, 98166 Messina, Italy.
authors.forename: D
authors.initials: D
authors.lastname: Barreca
authors.forename: E
authors.initials: E
authors.lastname: Bellocco
authors.forename: G
authors.initials: G
authors.lastname: Galli
authors.forename: G
authors.initials: G
authors.lastname: Laganà
authors.forename: U
authors.initials: U
authors.lastname: Leuzzi
authors.forename: S
authors.initials: S
authors.lastname: Magazù
authors.forename: F
authors.initials: F
authors.lastname: Migliardo
authors.forename: A
authors.initials: A
authors.lastname: Galtieri
authors.forename: Mark T F
authors.initials: MT
authors.lastname: Telling
mesh_terms: D000818:Animals; D003545:Cysteine; D018940:Elasmobranchii / Q000033:anatomy & histology; D004789:Enzyme Activation / Q000187:drug effects; D004795:Enzyme Stability / Q000187:drug effects; D007700:Kinetics; D008099:Liver / Q000201:enzymology; D009954:Ornithine Carbamoyltransferase / Q000737:chemistry* / Q000378:metabolism*; D012492:Salts / Q000494:pharmacology*; D013057:Spectrum Analysis; D013696:Temperature; D013816:Thermodynamics; D017186:Titrimetry
chemical_list: D012492:Salts; D009954:Ornithine Carbamoyltransferase; D003545:Cysteine