Synthesis and biological applications of two novel fluorescent proteins-labeling probes.
Two novel chlorinated fluoresceins 2',4',5',7'-tetrachloro-6-(5-carboxypentyl)-4,7-dichloro fluorescein succinimidyl ester (1G) and 2',4',5',7'-tetrachloro-6-(3-carboxypropyl)-4,7-dichlorofluorescein succinimidyl ester (2G) were synthesized as fluorescent probes for labeling proteins. Structures of target compounds and intermediates were determined via IR, MS, (1)H NMR and element analysis. The investigation in immunofluorescence histochemistry showed them had strong fluorescence, high photostability and good biocompatibility.
Wu
Xiang-Long
XL
Key Laboratory of Synthetic and Natural Functional Molecule Chemistry, Ministry of Education, College of Chemistry & Materials Science, Northwest University, Xi' an, China.
Tian
Min
M
He
Huai-Zhen
HZ
Sun
Wei
W
Li
Jian-Li
JL
Shi
Zhen
Z
D045744:Cell Line, Tumor; D005452:Fluoresceins / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology; D005456:Fluorescent Dyes / Q000138:chemical synthesis* / Q000737:chemistry; D006801:Humans; D011506:Proteins / Q000737:chemistry*; D013194:Staining and Labeling; D013388:Succinimides / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology
C541970:2',4',5',7'-tetrachloro-6-(5-carboxypentyl)-4,7-dichloro fluorescein succinimidyl ester; D005452:Fluoresceins; D005456:Fluorescent Dyes; D011506:Proteins; D013388:Succinimides