Effect of alkyl alcohols on partially unfolded state of proteinase K: Differential stability of alpha-helix and beta-sheet rich regions of the enzyme.
Proteinase K (E.C. 3.4.21.64), a serine proteinase from fungus Tritirachium album, has been used as a model system to investigate the conformational changes induced by monohydric alcohols at low pH. Proteinase K belongs to alpha/beta class of proteins and maintains structural integrity in the range of pH 7.0-3.0. Enzyme acquires partially unfolded conformation (U(P)) at pH 2.5 with lower activity, partial loss of tertiary structure and exposure of some hydrophobic patches. Proteinase K in stressed state at pH 2.5 is chosen and the conformational changes induced by alkyl alcohols (methanol/ethanol/isopropanol) are studied. At critical concentration of alcohol, conformational switch occurs in the protein structure from alpha/beta to beta-sheet driving the protein into O-state. Complete loss of tertiary contacts and proteolytic activity in O-sate emphasize the involvement of alpha regions in maintaining the active site of the enzyme. Moreover, isopropanol induced unfolding of proteinase K in U(P) state occurred in two steps with the formation of beta state at low alcohol concentration followed by stabilization of beta state at high alcohol concentration. GuHCl and temperature induced unfolding of proteinase K in O-state (in 50% isopropanol) is non-cooperative as the transition curves are biphasic. This suggests that the structure of proteinase K in O-state has melted alpha regions and stabilized beta regions and that these differentially stabilized regions unfold sequentially. Further, the O-state of proteinase K can be attained from complete unfolded protein by the addition of 50% isopropanol. Hence the alcohol-induced O-state is different from native state or completely unfolded state and shows characteristics of the molten globule-like state. Thus, this state may be functioning as an intermediary in the folding pathway of proteinase K.
Tomar
Ritu
R
Institute of Medical Sciences, Banaras Hindu University, Varanasi, India.
Dubey
Vikash Kumar
VK
Jagannadham
M V
MV
D019840:2-Propanol / Q000494:pharmacology; D000438:Alcohols / Q000737:chemistry* / Q000494:pharmacology*; D001203:Ascomycota / Q000201:enzymology; D002942:Circular Dichroism; D004305:Dose-Response Relationship, Drug; D019286:Endopeptidase K / Q000737:chemistry* / Q000378:metabolism; D004795:Enzyme Stability / Q000187:drug effects; D005456:Fluorescent Dyes / Q000378:metabolism; D019791:Guanidine / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D011489:Protein Denaturation / Q000187:drug effects; D017510:Protein Folding / Q000187:drug effects; D017433:Protein Structure, Secondary / Q000187:drug effects; D017434:Protein Structure, Tertiary / Q000187:drug effects; D013057:Spectrum Analysis; D013696:Temperature; D014867:Water / Q000737:chemistry
D000438:Alcohols; D005456:Fluorescent Dyes; D014867:Water; D019286:Endopeptidase K; D019791:Guanidine; D019840:2-Propanol