Sulfenic acid--a key intermediate in albumin thiol oxidation.
The single thiol of human serum albumin (HSA-SH) is the predominant plasma thiol. Both circulating albumin and pharmaceutical preparations are heterogeneous regarding the thiol redox status, as revealed by anion-exchange-hydrophobic interaction chromatography. Sulfenic acid (HSA-SOH) is an intermediate in HSA-SH oxidation processes that was detected through different techniques including mass spectrometry. Recently, quantitative data led to the determination of rate constants. The preferred fate of HSA-SOH is the formation of mixed disulfides. Alternatively, HSA-SOH can be further oxidized to sulfinic and sulfonic acids. Oxidized forms increase under disease conditions, underscoring the importance of HSA-SH as a plasma scavenger of intravascular oxidants. We here provide a critical review of the oxidation of HSA-SH in the context of the intravascular compartment, with emphasis in the methodological approaches of mass spectrometry and chromatography for the analysis of albumin thiol redox states.
Turell
Lucía
L
Laboratorio de Enzimología, Facultad de Ciencias, Universidad de la República, Montevideo, Uruguay.
Botti
Horacio
H
Carballal
Sebastián
S
Radi
Rafael
R
Alvarez
Beatriz
B
D002845:Chromatography; D006801:Humans; D013058:Mass Spectrometry; D010084:Oxidation-Reduction; D012709:Serum Albumin / Q000737:chemistry* / Q000378:metabolism; D013434:Sulfenic Acids / Q000737:chemistry*; D013438:Sulfhydryl Compounds / Q000737:chemistry*
D012709:Serum Albumin; D013434:Sulfenic Acids; D013438:Sulfhydryl Compounds