Effects of Er:YAG laser irradiation and manipulation treatments on dentin components, part 1: Fourier transform-Raman study.
The effects of laser etching, decontamination, and storage treatments on dentin components were studied using Fourier transform (FT)-Raman spectroscopy. Thirty bovine incisors were prepared to expose the dentin surface and then divided in two main groups based upon the decontamination process and storage procedure: autoclaved (group A, n=15) or stored in thymol aqueous solution (group B, n=15). The surfaces of the dentin slices were schematically divided into four areas, with each one corresponding to a treatment subgroup. The specimens were either etched with phosphoric acid (control subgroup) or irradiated with erbium-doped yttrium-aluminum-garnet (Er:YAG) laser (subgroups: I-80 mJ, II-120 mJ, and III-180 mJ, and total energy of 12 J). Samples were analyzed by FT-Raman spectroscopy; we collected three spectra for each area (before and after treatment). The integrated areas of five Raman peaks were calculated to yield average spectra. The areas of the peaks associated with phosphate content (P<0.001), type I collagen, and organic C-H bonds (P<0.05) were reduced significantly in group A (control). Analyses of samples irradiated with reduced laser energies did not show significant changes in the dentin components. These results suggest that thymol storage treatment is advised for in vitro study; furthermore, 12 J of Er:YAG laser energy does not affect dentin components.
Soares
Luís Eduardo Silva
LE
Vale do Paraiba University, UNIVAP, Dental Materials and Operative Dentistry Department, School of Dentistry Research and Development Institute, 12244-000 Sao Jose dos Campos, Sao Paulo, Brazil. lesoares@univap.br
do Espírito Santo
Ana Maria
AM
Junior
Aldo Brugnera
AB
Zanin
Fátima Anto Nia Aparecida
FA
da Silva Carvalho
Carolina
C
de Oliveira
Rodrigo
R
Martin
Airton Abrahão
AA
D000818:Animals; D002417:Cattle; D003804:Dentin / Q000737:chemistry* / Q000528:radiation effects*; D004307:Dose-Response Relationship, Radiation; D066298:In Vitro Techniques; D007180:Incisor / Q000737:chemistry / Q000528:radiation effects; D053844:Lasers, Solid-State; D011829:Radiation Dosage; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods*; D013059:Spectrum Analysis, Raman / Q000379:methods*