Enterovirus detection in stool specimen: relevance for poliovirus and enterovirus surveillance.
Detection of enterovirus genome by PCR in clinical samples is now extensively used for the diagnostic of enterovirus infections given its rapidity and high sensitivity. In contrast, its use in surveillance programs targeting specific enterovirus serotypes remains less frequent. The most sensitive protocols are those amplifying in the 5'untranslated region (5'UTR). However the possibility to use sequence analysis of the 5'UTR amplicons for serotype identification is not yet well established. In this report, stool samples from polio suspected cases and their healthy contacts were tested. The results of direct detection of enterovirus genome by PCR and serotype identification based on sequence analysis of the PCR products in the 5'UTR were compared to those of standard cell-culture-based protocols. Standard protocols detected enterovirus isolates in 7.4% of cases while 9.8% of samples were positive by PCR. Serotype identification based on sequence analysis of amplicons showed concordant results with serotypes determined on virus isolates by seroneutralisation or sequencing in the VP1 gene in 39% of cases only. These results confirm that the use of PCR amplification from stool samples improves the sensitivity of enterovirus detection but do not recommend the use of sequence analysis of the 5'UTR PCR product to determine enterovirus serotype.
Haddad-Boubaker
S
S
Laboratory of Clinical Virology (WHO Regional Reference Laboratory for Poliomyelitis and Measles), Institut Pasteur de Tunis, 13 Place Pasteur, BP 74, 1002 Tunis, Tunisia. sondes_hadded@yahoo.fr
Yahia
A Ben
AB
Rezig
D
D
Farès
W
W
Touzi
H
H
Triki
H
H
D020121:5' Untranslated Regions / Q000235:genetics*; D016022:Case-Control Studies; D004770:Enterovirus / Q000145:classification / Q000235:genetics*; D004769:Enterovirus Infections / Q000175:diagnosis / Q000453:epidemiology / Q000821:virology*; D005243:Feces / Q000821:virology*; D016679:Genome, Viral / Q000235:genetics; D006801:Humans; D017720:Molecular Epidemiology; D011051:Poliomyelitis / Q000453:epidemiology / Q000821:virology; D017955:Poliovirus / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods*; D011159:Population Surveillance; D012367:RNA, Viral / Q000235:genetics*; D012680:Sensitivity and Specificity; D012703:Serotyping; D014416:Tunisia / Q000453:epidemiology; D014776:Virus Cultivation / Q000379:methods
D020121:5' Untranslated Regions; D012367:RNA, Viral