Imaging of mRNA in live cells using nucleic acid-templated reduction of azidorhodamine probes.
Nucleic acid-templated reactions leading to a fluorescent product represent an attractive strategy for the detection and imaging of cellular nucleic acids. Herein we report the use of a Staudinger reaction to promote the reduction of profluorescent azidorhodamine. The use of two cell-permeable GPNA probes, one labeled with the profluorescent azidorhodamine and the other with trialkylphosphine, enabled the detection of the mRNA encoding O-6-methylguanine-DNA methyltransferase in intact cells.
Pianowski
Zbigniew
Z
Institut de Science et Ingenierie Supramoleculaires (ISIS-UMR 7006), Universite de Strasbourg-CNRS, 8 allee Gaspard Monge, 67000 Strasbourg, France.
Gorska
Katarzyna
K
Oswald
Laurence
L
Merten
Christoph A
CA
Winssinger
Nicolas
N
D002460:Cell Line; D002477:Cells / Q000737:chemistry / Q000166:cytology*; D015342:DNA Probes; D003952:Diagnostic Imaging / Q000379:methods; D005456:Fluorescent Dyes; D006801:Humans; D015336:Molecular Probe Techniques; D019853:O(6)-Methylguanine-DNA Methyltransferase / Q000032:analysis; D012333:RNA, Messenger / Q000032:analysis*; D012235:Rhodamines
D015342:DNA Probes; D005456:Fluorescent Dyes; D012333:RNA, Messenger; D012235:Rhodamines; D019853:O(6)-Methylguanine-DNA Methyltransferase