title: High-throughput generation of tagged stable cell lines for proteomic analysis.
abstract: We present an optimized system for rapid generation of localization and affinity purification-tagged mammalian stable cell lines that facilitates complex purification and interacting protein identification. The improved components of this method, including the flexibility of inducible expression, circumvent issues associated with toxicity, clonal selection, sample yields and time to data acquisition. We have applied this method to the study of cell-cycle regulators and novel microtubule-associated proteins.
authors.affiliation: Department of Pathology, Stanford University School of Medicine, Stanford, CA, USA. jorget@gene.com
authors.forename: Jorge Z
authors.initials: JZ
authors.lastname: Torres
authors.forename: Julie J
authors.initials: JJ
authors.lastname: Miller
authors.forename: Peter K
authors.initials: PK
authors.lastname: Jackson
mesh_terms: D000818:Animals; D002460:Cell Line; D003001:Cloning, Molecular; D008322:Mammals; D011506:Proteins / Q000032:analysis* / Q000302:isolation & purification / Q000378:metabolism; D040901:Proteomics / Q000379:methods*; D011993:Recombinant Fusion Proteins / Q000302:isolation & purification / Q000378:metabolism
chemical_list: D011506:Proteins; D011993:Recombinant Fusion Proteins