Patterning of HeLa cells on a microfabricated Au-coated ITO substrate.
HeLa cells were patterned on a microfabricated Au-coated ITO substrate. Part of the Au film was dissolved by the electrochemical wet stamping technique, and the substrate was then immersed in a thiol-terminated methoxy-poly(ethylene glycol) (mPEG-SH) solution for further modification. Self-assembled monolayers (SAMs) on the gold island can form cell- and protein-resistant regions on the substrate, and living HeLa cells can adhere to the exposed ITO surface. Single cell patterns could be achieved when the size of a microstructure unit matched that of the cell. The fluorescence experiments further proved that living HeLa cells prefer to attach to the ITO substrate rather than the mPEG-SH-modified gold islands.
Jin
Li H
LH
Department of Chemistry, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, 361005, China.
Yang
Bing Y
BY
Zhang
Li
L
Lin
Pei L
PL
Cui
Chen
C
Tang
Jing
J
D002448:Cell Adhesion; D018914:Cells, Immobilized / Q000166:cytology / Q000378:metabolism*; D006046:Gold / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D007204:Indium / Q000737:chemistry*; D013499:Surface Properties; D017971:Tin Compounds / Q000737:chemistry*
D017971:Tin Compounds; D007204:Indium; D006046:Gold; C045358:stannic oxide