title: Seeding-induced self-assembling protein nanowires dramatically increase the sensitivity of immunoassays.
abstract: Aiming to build a supersensitive and easily operable immunoassay, bifunctional protein nanowires were generated by seeding-induced self-assembling of the yeast amyloid protein Sup35p that genetically fused with protein G and an enzyme (methyl-parathion hydrolase, MPH), respectively. The protein nanowires possessed a high ratio of enzyme molecules to protein G, allowing a dramatic increase of the enzymatic signal when protein G was bound to an antibody target. As a result, a 100-fold enhancement of the sensitivity was obtained when applied in the detection of the Yersinia pestis F1 antigen.
authors.affiliation: State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, China.
authors.forename: Dong
authors.initials: D
authors.lastname: Men
authors.forename: Yong-Chao
authors.initials: YC
authors.lastname: Guo
authors.forename: Zhi-Ping
authors.initials: ZP
authors.lastname: Zhang
authors.forename: Hong-ping
authors.initials: HP
authors.lastname: Wei
authors.forename: Ya-Feng
authors.initials: YF
authors.lastname: Zhou
authors.forename: Zong-Qiang
authors.initials: ZQ
authors.lastname: Cui
authors.forename: Xiao-Sheng
authors.initials: XS
authors.lastname: Liang
authors.forename: Ke
authors.initials: K
authors.lastname: Li
authors.forename: Yan
authors.initials: Y
authors.lastname: Leng
authors.forename: Xiang-Yu
authors.initials: XY
authors.lastname: You
authors.forename: Xian-En
authors.initials: XE
authors.lastname: Zhang
mesh_terms: D001426:Bacterial Proteins / Q000032:analysis; D004797:Enzyme-Linked Immunosorbent Assay; D053770:Nanowires / Q000737:chemistry*; D009419:Nerve Tissue Proteins / Q000737:chemistry / Q000235:genetics; D010454:Peptide Termination Factors / Q000737:chemistry / Q000235:genetics; D010744:Phosphoric Monoester Hydrolases / Q000737:chemistry / Q000235:genetics; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry / Q000235:genetics; D012680:Sensitivity and Specificity
chemical_list: D001426:Bacterial Proteins; C029152:G-substrate; D009419:Nerve Tissue Proteins; D010454:Peptide Termination Factors; D011994:Recombinant Proteins; C068068:SUP35 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; C086725:caf1 protein, Yersinia pestis; D010744:Phosphoric Monoester Hydrolases