[Research on DNA fluorescence capillary biosensor marked by Goldview].
Goldview marked DNA fluorescence capillary biosensor was studied in the present paper. Based on fluorescence capillary analysis (FCA), the DNA biosensor uses capillary as immobilization carrier and detection carrier of DNA probe. Probes (20-mer-ssDNA) were immobilized on the inner wall of capillary by poly-l-lysine, and DNA fluorescence capillary biosensor (DNA-FCB) was made. After being hybridized with complementary target DNA and dyed by Goldview, the target DNA was qualified or quantified by detecting the fluorescence density of the Goldview using F-4500 spectrofluorometer. The sample volume was 12 microL. The concentration of the target DNA showed good linearity with the fluorescence intensity in the range of 0. 4-4 micromol x L(-1) (2.4-24 mg x L(-1)) (y = 65.911x + 3.9944, r = 0.9989). The RSD was lower than 3.5%. The concentration detection limit of the target DNA was 0.39 micromol x L(-1) (2.2 mg x L(-1)). The DNA-FCB can be used to qualify or quantify the target DNA. It's advantages are simplicity of manipulation, thimbleful of sample and reagent volumes, repeated use of capillary, and the lowest test cost. By using DNA-FCB to qualify the target DNA, we can consumedly decrease the pollution of the environment.
Wang
Yan-Jun
YJ
West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China.
Li
Yong-Sheng
YS
Yang
Quan-Yu
QY
Huang
Yi
Y
Tang
Jing
J
Gao
Xiu-Feng
XF
D015374:Biosensing Techniques / Q000379:methods*; D002196:Capillaries; D004247:DNA; D015342:DNA Probes; D004800:Enzymes, Immobilized; D005336:Fiber Optic Technology / Q000379:methods*; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000032:analysis*; D055100:Optical Fibers / Q000706:statistics & numerical data; D012106:Research; D013050:Spectrometry, Fluorescence
D015342:DNA Probes; D004800:Enzymes, Immobilized; D005456:Fluorescent Dyes; D004247:DNA