The apoptosis linked gene ALG-2 is dysregulated in tumors of various origin and contributes to cancer cell viability.
The apoptosis linked gene-2 (ALG-2), discovered as a proapoptotic calcium binding protein, has recently been found upregulated in lung cancer tissue indicating that this protein may play a role in the pathology of cancer cells and/or may be a tumor marker. Using immunohistochemistry on tissue microarrays we analysed the expression of ALG-2 in 7371 tumor tissue samples of various origin as well as in 749 normal tissue samples. Most notably, ALG-2 was upregulated in mesenchymal tumors. No correlation was found between ALG-2 staining intensity and survival of patients with lung, breast or colon cancer. siRNA mediated ALG-2 downregulation led to a significant reduction in viability of HeLa cells indicating that ALG-2 may contribute to tumor development and expansion.
la Cour
Jonas M
JM
Copenhagen Biocenter, Department of Molecular Biology, University of Copenhagen, Ole Maaløes Vej 5, Copenhagen N 2200, Denmark.
Høj
Berit R
BR
Mollerup
Jens
J
Simon
Ronald
R
Sauter
Guido
G
Berchtold
Martin W
MW
D017209:Apoptosis / Q000235:genetics*; D051017:Apoptosis Regulatory Proteins / Q000235:genetics*; D001943:Breast Neoplasms; D002135:Calcium-Binding Proteins / Q000235:genetics*; D002470:Cell Survival / Q000235:genetics; D003110:Colonic Neoplasms; D015536:Down-Regulation / Q000187:drug effects; D015972:Gene Expression Regulation, Neoplastic; D006367:HeLa Cells; D006801:Humans; D008175:Lung Neoplasms; D008648:Mesoderm / Q000473:pathology; D009369:Neoplasms / Q000235:genetics / Q000473:pathology*; D034741:RNA, Small Interfering / Q000494:pharmacology; D046888:Tissue Array Analysis
D051017:Apoptosis Regulatory Proteins; D002135:Calcium-Binding Proteins; C097753:PDCD6 protein, human; D034741:RNA, Small Interfering