title: Function of the N-terminal region of the phosphate-sensing histidine kinase, SphS, in Synechocystis sp. PCC 6803.
abstract: In Synechocystis sp. PCC 6803 the histidine kinase SphS (sll0337) is involved in transcriptional activation of the phosphate (Pi)-acquisition system which includes alkaline phosphatase (AP). The N-terminal region of SphS contains both a hydrophobic region and a Per-Arnt-Sim (PAS) domain. The C-terminal region has a highly conserved transmitter domain. Immunological localization studies on heterologously expressed SphS in Escherichia coli indicate that the hydrophobic region is important for membrane localization. In order to evaluate the function of the N-terminal region of SphS, deletion mutants under the control of the native promoter were analysed for in vivo AP activity. Deletion of the N-terminal hydrophobic region resulted in loss of AP activity under both Pi-deficient and Pi-sufficient conditions. Substitution of the hydrophobic region of SphS with that from the Ni2+-sensing histidine kinase, NrsS, resulted in the same induction characteristics as SphS. Deletion of the PAS domain resulted in the constitutive induction of AP activity regardless of Pi availability. To characterize the PAS domain in more in detail, four amino acid residues conserved in the PAS domain were substituted with Ala. Among the mutants R121A constitutively expressed AP activity, suggesting that R121 is important for the function of the PAS domain. Our observations indicated that the presence of a transmembrane helix in the N-terminal region of SphS is critical for activity and that the PAS domain is involved in perception of Pi availability.
authors.affiliation: Graduate School of Life and Environmental Sciences, University of Tsukuba, Tennodai 1-1-1, Tsukuba 305-8572 Japan.
authors.forename: Satoshi
authors.initials: S
authors.lastname: Kimura
authors.affiliation: Graduate School of Life and Environmental Sciences, University of Tsukuba, Tennodai 1-1-1, Tsukuba 305-8572 Japan.
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Shiraiwa
authors.affiliation: Graduate School of Life and Environmental Sciences, University of Tsukuba, Tennodai 1-1-1, Tsukuba 305-8572 Japan.
authors.forename: Iwane
authors.initials: I
authors.lastname: Suzuki
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D000595:Amino Acid Sequence; D019943:Amino Acid Substitution; D001426:Bacterial Proteins / Q000737:chemistry / Q000502:physiology; D004789:Enzyme Activation; D000071677:Histidine Kinase; D008565:Membrane Proteins / Q000378:metabolism; D008969:Molecular Sequence Data; D010770:Phosphotransferases / Q000737:chemistry / Q000502:physiology; D011494:Protein Kinases / Q000378:metabolism; D017434:Protein Structure, Tertiary / Q000502:physiology; D017384:Sequence Deletion; D046939:Synechocystis / Q000378:metabolism*; D014157:Transcription Factors / Q000737:chemistry / Q000502:physiology
chemical_list: D001426:Bacterial Proteins; D008565:Membrane Proteins; D014157:Transcription Factors; D010770:Phosphotransferases; D011494:Protein Kinases; D000071677:Histidine Kinase; C081252:SphS protein, Synechococcus sp.; D000469:Alkaline Phosphatase