title: Female segregation patterns of the putative Y-chromosome-specific microsatellite markers INRA124 and INRA126 do not support their use for cattle population studies.
abstract: Here we tested the segregation and paternal compatibility of markers INRA124 and INRA126 on female DNA in 10 different cattle families, in order to clarify the usefulness of these microsatellites for the study of male-mediated population processes in cattle. Their performance was compared with that of four microsatellites located in the PAR-BTAY (UMN0108, UMN0803, UMN0929 and UMN0905) and another one male-specific microsatellite (INRA189). INRA124 and INRA126 amplified the same sized fragment in both sexes. Same size alleles were sequenced and the high homology found allowed us to rule out non-specific female amplification. INRA124 showed full parental compatibility, whilst the locus INRA126 showed 55% parental incompatibility. Based on these observations, it is recommended that markers INRA124 and INRA126 should not be used in studies to characterize male-mediated genetic events in cattle.
authors.affiliation: SERIDA-Somió, C/ Camino de los Claveles 604, E-33203 Gijón, Asturias, Spain.
authors.forename: L
authors.initials: L
authors.lastname: Pérez-Pardal
authors.forename: L J
authors.initials: LJ
authors.lastname: Royo
authors.forename: I
authors.initials: I
authors.lastname: Alvarez
authors.forename: F A Ponce
authors.initials: FA
authors.lastname: de León
authors.forename: I
authors.initials: I
authors.lastname: Fernández
authors.forename: R
authors.initials: R
authors.lastname: Casais
authors.forename: F
authors.initials: F
authors.lastname: Goyache
mesh_terms: D000818:Animals; D002417:Cattle / Q000235:genetics*; D020090:Chromosome Segregation; D005260:Female; D005819:Genetic Markers; D005838:Genotype; D008297:Male; D018895:Microsatellite Repeats; D010375:Pedigree; D017422:Sequence Analysis, DNA; D014998:Y Chromosome
chemical_list: D005819:Genetic Markers