Staphylococcus cohnii hemolysins - isolation, purification and properties.
A total 355 of Staphylococcus cohnii isolates from hospital environment, patients (newborns), medical staff and from non-hospital environment were tested for hemolytic activity. Ninety-one % of S. cohnii ssp. cohnii and 74.5 % S. cohnii ssp. urealyticus strains exhibited hemolysis synergistic to S. aureus ATCC 25923 strain. Crude preparations of hemolysins of both bacterial subspecies presented delta-hemolysin, but not alpha- and beta-toxin activity. Highly pure hemolysins were obtained by semipreparative SDS-PAGE or by organic solvent extraction from the freeze-dried crude preparations. Native-PAGE and 2D-PAGE showed their high heterogeneity. Molar masses of single hemolysin units estimated by the Tris-Tricine-SDS-PAGE were calculated as 3.47 kDa for S. cohnii ssp. cohnii and 3.53 kDa for S. cohnii ssp. urealyticus.
Rózalska
M
M
Department of Pharmaceutical Microbiology, Medical University of Łódź, Poland. mrozalska@pharm.am.lodz.pl
Szewczyk
E M
EM
D000328:Adult; D003428:Cross Infection / Q000382:microbiology; D004591:Electrophoresis, Polyacrylamide Gel; D006460:Hemolysin Proteins / Q000302:isolation & purification* / Q000378:metabolism; D006801:Humans; D007231:Infant, Newborn; D007525:Isoelectric Focusing; D008970:Molecular Weight; D010564:Personnel, Hospital; D011044:Poland; D012867:Skin / Q000382:microbiology; D013203:Staphylococcal Infections / Q000382:microbiology; D013210:Staphylococcus / Q000737:chemistry* / Q000302:isolation & purification
D006460:Hemolysin Proteins