[Culture optimization for protein expression in Escherichia coli].
OBJECTIVE
We developed a new culture-complex auto-inducing media (CAI) for heterogenous protein expression in Escherichia coli.


METHODS
To test expression efficiency in the CAI, we constructed seven different plasmids named p-1, p-2, p-3, p-4, p-5, p-6 and p-7. These plasmids were transformed into E. coli BL21, then expressed in both Luria-Bertani madia LB and CAI. To improve the expression level even more, we analyzed the composition of the CAI and optimized the culture.


RESULTS
The expression levels of seven fusion proteins in CAI were four times higher than those in Luria-Bertani. Through a series of changes we formed a new optimized culture (CAI-4).


CONCLUSIONS
Comparing to the expression levels of these fusion proteins (P-1, P-2, P-3) in CAI, the expression levels of fusion proteins in CAI-4 increased 2-fold.
Xu
Linglong
L
Division of Avian Infectious Diseases, National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, China.
Shi
Xingming
X
Wang
Yunfeng
Y
Sun
Yan
Y
Wang
Mei
M
D001709:Biotechnology; D018929:Cell Culture Techniques; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial
D029968:Escherichia coli Proteins