A high-performance liquid chromatographic analysis and preliminary pharmacokinetic characterization of 3'-hydroxypterostilbene in rats.
A high-performance liquid chromatographic method was developed for the analysis of 3'-hydroxypterostilbene. This method involves the use of a Luna C(18) column with ultraviolet detection at 325 nm. The mobile phase consisted of acetonitrile, water and formic acid (50:50:0.01, v/v/v) with a flow rate of 0.8 mL/min. The calibration curves were linear over the range 0.5-100.0 microg/mL. The mean extraction efficiency was between 97.40 and 111.16%. The precision of the assay was 0.196-14.39% (RSD%), and within 15% at the limit of quantitation (0.5 microg/mL). The bias of the assay was <16% and within 15% at the limit of quantitation. This assay was successfully applied to pre-clinical pharmacokinetic samples from rat urine and serum.
Takemoto
Jody K
JK
College of Pharmacy Department of Pharmaceutical Sciences and Pharmacology and Toxicology Graduate Program Washington State University, Pullman, WA 99164-6534, USA.
Davies
Neal M
NM
D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004355:Drug Stability; D016014:Linear Models; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013267:Stilbenes / Q000032:analysis* / Q000097:blood / Q000493:pharmacokinetics* / Q000652:urine
C505072:3'-hydroxypterostilbene; D013267:Stilbenes