Liquid chromatographic-mass spectrometry analysis and pharmacokinetic studies of erianin for intravenous injection in dogs.
The purpose of the present study was to examine the pharmacokinetic characteristics of erianin (2-methoxy-5-[2-(3,4,5-trimethoxyphenyl)-ethyl]-phenol, CAS 95041-90-0), a nature product extracted from Dendrobium chrysotoxum, having notable antitumour activity, after intravenous injection of erianin fat emulsion to beagle dogs. An HPLC-MS method was developed to analyze the erianin levels in dog plasma and validated in a pharmacokinetic study. Plasma profiles were obtained after intravenous injection of erianin fat emulsion at the doses 7.5, 15 and 30 mg/kg. The elimination half-life (t(1/2)) values for erianin were estimated to be 1.41+/- 0.31, 1.66 +/- 0.19, 1.60 0.28 h, while the mean area under concentration-time curve (AUC(0-infinity)) values were 1021.3 +/- 373.7, 2305.1 +/- 597.0 and 3952.1 +/- 378.2 ng x h/ml, respectively. In conclusion, the present observations indicated that erianin plasma concentrations were clearly dose-proportional for the dose range studied. There was no gender difference in pharmacokinetics for erianin in male and female dogs.
Zhou
Honghua
H
Center for Instrumental Analysis, China Pharmaceutical University, Tongjiaxiang 24, Nanjing, Jiangsu Province, 210009, The People's Republic of China. drzhh@126.com
Yang
Bingxun
B
Hong
Min
M
Ma
Renling
R
Sheng
Longsheng
L
D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000008:administration & dosage / Q000493:pharmacokinetics*; D019540:Area Under Curve; D001632:Bibenzyls / Q000008:administration & dosage / Q000493:pharmacokinetics*; D002851:Chromatography, High Pressure Liquid; D004285:Dogs; D005217:Fat Emulsions, Intravenous; D005260:Female; D006207:Half-Life; D007202:Indicators and Reagents; D007275:Injections, Intravenous; D008297:Male; D013058:Mass Spectrometry; D019800:Phenol; D015203:Reproducibility of Results; D012727:Sex Characteristics
D000972:Antineoplastic Agents, Phytogenic; D001632:Bibenzyls; C477638:Erianin; D005217:Fat Emulsions, Intravenous; D007202:Indicators and Reagents; D019800:Phenol