title: Plasma levels of FL and SDF-1 and expression of FLT-3 and CXCR4 on CD34+ cells assessed pre and post hematopoietic stem cell mobilization in patients with hematologic malignancies and in healthy donors.
abstract: Peripheral blood stem cells (PBSC) became the main source of cells for autologous transplantation. Alterations in the expression of adhesion molecules are essential in the CD34+ cells mobilization process. These molecules are involved in the interaction between hematopoietic and stromal cells and they have been disclosed as a considerable factor to the trafficking and homing of the CD34+ progenitor cells. This is a non-randomized PBSC mobilization study designed to evaluate the influence and behavior of FL and SDF-1 and their receptors in two different moments, prior and after HPCs mobilization, with the yield of CD34+ cells collected by apheresis. There was higher concentration of FL and lower of SDF-1 plasma level at post than pre PBSC mobilization (p=0.001 and p=0.012, respectively) regarding all individuals searched, but without any correlation with a good yield of CD34+ cells. However, CXCR4 expressions on the CD34+ cells from bone marrow aspirates (BMA), at pre and post mobilization showed a difference statistical significant for those individuals with good yield of CD34+ cells (p=0,036), but not achieved for poor yield (p=0,156). There was a higher expression of CXCR4 in steady-state for the successfully individuals than for those unsuccessfully (529.84+/-54.68 and 496.31+/-97.51, respectively). In conclusion, we confirmed the important role of CXCR4/SDF-1 axis in the process of PBSC mobilization.
authors.affiliation: UNIFESP-Universidade Federal de São Paulo, Department of Clinical and Experimental Oncology, Rua Botucatu 740, Blood Bank 4th Floor, CEP-04023-902 São Paulo SP, Brazil. cecyn@uol.com.br
authors.forename: Karin Zattar
authors.initials: KZ
authors.lastname: Cecyn
authors.forename: Dulce Marta
authors.initials: DM
authors.lastname: Schimieguel
authors.forename: Eliza Yuriko Sugano
authors.initials: EY
authors.lastname: Kimura
authors.forename: Mihoko
authors.initials: M
authors.lastname: Yamamoto
authors.forename: José Salvador Rodrigues de
authors.initials: JS
authors.lastname: Oliveira
mesh_terms: D000328:Adult; D018952:Antigens, CD34; D001856:Bone Marrow Examination; D054377:Chemokine CXCL12 / Q000097:blood*; D015536:Down-Regulation; D005260:Female; D019650:Hematopoietic Stem Cell Mobilization; D006412:Hematopoietic Stem Cells / Q000378:metabolism*; D006801:Humans; D008297:Male; D008875:Middle Aged; D036102:Peripheral Blood Stem Cell Transplantation; D019718:Receptors, CXCR4 / Q000097:blood / Q000378:metabolism*; D055815:Young Adult; D051941:fms-Like Tyrosine Kinase 3 / Q000097:blood*
chemical_list: D018952:Antigens, CD34; D054377:Chemokine CXCL12; D019718:Receptors, CXCR4; C497970:FLT3 protein, human; D051941:fms-Like Tyrosine Kinase 3