OH-2, a hyperdiploid myeloma cell line without an IGH translocation, has a complex translocation juxtaposing MYC near MAFB and the IGK locus.
Multiple myeloma can be classified into hyperdiploid (HRD) (with 48-74 chromosomes) and non-hyperdiploid tumors (usually with immunoglobulin heavy chain translocations). The OH-2 human myeloma cell line (HMCL) retains the same HRD genotype as the primary tumor, with extra copies of chromosomes 3, 7, 15, 19, and 21. Both OH-2 and primary cells have a complex secondary translocation in which the IGK 3' enhancer is inserted between MYC and MAFB, resulting in dysregulation of both oncogenes. OH-2 provides a unique example of an HMCL and the corresponding primary tumor that are shown to share the same HRD genotype.
Våtsveen
Thea Kristin
TK
Department of Cancer Research and Molecular Medicine, Faculty of Medicine, Norwegian University of Science and Technology, Trondheim, Norway. thea.k.vatsveen@ntnu.no
Tian
Erming
E
Kresse
Stine H
SH
Meza-Zepeda
Leonardo A
LA
Gabrea
Ana
A
Glebov
Oleg
O
Dai
Hong Yan
HY
Sundan
Anders
A
Kuehl
W Michael
WM
Børset
Magne
M
D045744:Cell Line, Tumor; D004171:Diploidy; D004742:Enhancer Elements, Genetic; D016259:Genes, myc; D005838:Genotype; D006801:Humans; D007143:Immunoglobulin Heavy Chains / Q000235:genetics*; D007145:Immunoglobulin kappa-Chains / Q000235:genetics*; D017404:In Situ Hybridization, Fluorescence; D007621:Karyotyping; D051258:MafB Transcription Factor / Q000235:genetics*; D009101:Multiple Myeloma / Q000235:genetics* / Q000473:pathology; D012333:RNA, Messenger / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014178:Translocation, Genetic
D007143:Immunoglobulin Heavy Chains; D007145:Immunoglobulin kappa-Chains; C490407:MAFB protein, human; D051258:MafB Transcription Factor; D012333:RNA, Messenger