Generation of rat monoclonal antibodies specific for Ad4BP/SF-1.
Ad4BP/SF-1 (adrenal4 binding protein/steroidogenic factor-1[NR5A1]) is an essential nuclear receptor required for animal reproduction and endocrine regulation. The present study reports on monoclonal antibodies (MAbs) directed against mouse Ad4BP/SF-1, which were produced by the hybridization of mouse myeloma cells with lymph node cells of an immunized rat. The produced MAbs reacted with both recombinant and endogenous Ad4BP/SF-1. These MAbs will be useful in immunolocalization and immunoblotting experiments conducted on different tissue types to determine the levels of expression of Ad4BP/SF-1 throughout development, as well as further analyses of the biological function and cellular dynamics of this protein.
Yokoyama
Chikako
C
Department of Bioengineering, Graduate School of Engineering, Osaka City University, Osaka, Japan.
Komatsu
Tomoko
T
Ogawa
Hidesato
H
Morohashi
Ken-ichirou
K
Azuma
Masayuki
M
Tachibana
Taro
T
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D002460:Cell Line; D047369:Chromatin Immunoprecipitation; D003001:Cloning, Molecular; D004797:Enzyme-Linked Immunosorbent Assay; D019084:Fluorescent Antibody Technique, Indirect; D006801:Humans; D015151:Immunoblotting; D051379:Mice; D051381:Rats; D054339:Steroidogenic Factor 1 / Q000276:immunology*
D000911:Antibodies, Monoclonal; D054339:Steroidogenic Factor 1