title: Simultaneous determination of voriconazole and posaconazole concentrations in human plasma by high-performance liquid chromatography.
abstract: A simple, sensitive, and selective high-performance liquid chromatographic method for the simultaneous determination of voriconazole and posaconazole concentrations in human plasma was developed and validated. Quantitative recovery following liquid-liquid extraction with diethyl ether was achieved. Linearity ranged from 0.10 to 20.0 microg/ml for voriconazole and from 0.05 to 10.0 microg/ml for posaconazole. The intra- and interday coefficients of variation were less than 8.5%, and the lower limits of quantitation were <0.05 microg/ml.
authors.affiliation: Department of Internal Medicine II, Division of Infectious Diseases, University of Wuerzburg Medical Center, Josef-Schneider Strasse 2, Wuerzburg, Germany.
authors.forename: Kathrin
authors.initials: K
authors.lastname: Kahle
authors.forename: Peter
authors.initials: P
authors.lastname: Langmann
authors.forename: Diana
authors.initials: D
authors.lastname: Schirmer
authors.forename: Ulrike
authors.initials: U
authors.lastname: Lenker
authors.forename: Daniela
authors.initials: D
authors.lastname: Keller
authors.forename: Annegret
authors.initials: A
authors.lastname: Helle
authors.forename: Hartwig
authors.initials: H
authors.lastname: Klinker
authors.forename: Werner J
authors.initials: WJ
authors.lastname: Heinz
mesh_terms: D000935:Antifungal Agents / Q000097:blood*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D011743:Pyrimidines / Q000097:blood*; D015203:Reproducibility of Results; D014230:Triazoles / Q000097:blood*; D065819:Voriconazole
chemical_list: D000935:Antifungal Agents; D011743:Pyrimidines; D014230:Triazoles; C101425:posaconazole; D065819:Voriconazole