Selective recognition of thymidine homopolymer (poly T) oligonucleotide with cobalt(II)-4-[(5-chloro-2-pyridyl)azo]-1,3-diaminobenzene complex.
The interactions of cobalt(II)-4-[(5-chloro-2-pyridyl)azo]-1,3-diaminobenzene (5-Cl-PADAB) complex with different kinds of homopolymer oligonucleotides in basic medium were investigated based on the measurements of resonance light scattering, UV-vis, circular dichroism spectra and dark field light-scattering imaging. Experiments showed that only thymidine homopolymer (poly T) oligonucleotides with the length in the range of poly T6 to poly T18 could interact with the Co(II)-5-Cl-PADAB complex in alkaline conditions and cause evident color and spectral change. Thus, the binary complex of Co(II)-5-Cl-PADAB could be employed as a visual probe for selectively recognizing the poly T oligonucleotides.
Guo
Feng Ling
FL
College of Chemistry and Chemical Engineering, MOE Key Laboratory on Luminescence and Real-Time Analysis, Southwest University, Chongqing 400715, People's Republic of China.
Li
Yuan Fang
YF
Huang
Cheng Zhi
CZ
D002942:Circular Dichroism; D006863:Hydrogen-Ion Concentration; D008027:Light; D009841:Oligonucleotides / Q000737:chemistry*; D009942:Organometallic Compounds / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*; D012542:Scattering, Radiation; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D013936:Thymidine / Q000737:chemistry*
D009841:Oligonucleotides; D009942:Organometallic Compounds; D011108:Polymers; C543422:cobalt(II)-4-((5-chloro-2-pyridyl)azo)-1,3-diaminobenzene; D013936:Thymidine