title: Analysis of anabolic steroids in hair: time courses in guinea pigs.
abstract: Sensitive, specific, and reproducible methods for the quantitative determination of eight anabolic steroids in guinea pig hair have been developed using LC/MS/MS and GC/MS/MS. Methyltestosterone, stanozolol, methandienone, nandrolone, trenbolone, boldenone, methenolone and DHEA were administered intraperitoneally in guinea pigs. After the first injection, black hair segments were collected on shaved areas of skin. The analysis of these segments revealed the distribution of anabolic steroids in the guinea pig hair. The major components in hair are the parent anabolic steroids. The time courses of the concentrations of the steroids in hair (except methenolone, which does not deposit in hair) demonstrated that the peak concentrations were reached on days 2-4, except stanozolol, which peaked on day 10 after administration. The concentrations in hair appeared to be related to the physicochemical properties of the drug compound and to the dosage. These studies on the distribution of drugs in the hair shaft and on the time course of their concentration changes provide information relevant to the optimal time and method of collecting hair samples. Such studies also provide basic data that will be useful in the application of hair analysis in the control of doping and in the interpretation of results.
authors.affiliation: Department of Forensic Toxicology, Institute of Forensic Sciences, Ministry of Justice, Shanghai Key laboratory of Forensic Medicine, Shanghai, PR China. minshensfjd@hotmail.com
authors.forename: Min
authors.initials: M
authors.lastname: Shen
authors.forename: Ping
authors.initials: P
authors.lastname: Xiang
authors.forename: Hui
authors.initials: H
authors.lastname: Yan
authors.forename: Baohua
authors.initials: B
authors.lastname: Shen
authors.forename: Mengye
authors.initials: M
authors.lastname: Wang
mesh_terms: D045930:Anabolic Agents / Q000008:administration & dosage / Q000032:analysis* / Q000378:metabolism / Q000493:pharmacokinetics*; D000818:Animals; D006168:Guinea Pigs; D006197:Hair / Q000737:chemistry*; D006801:Humans; D007274:Injections, Intraperitoneal; D016014:Linear Models; D008297:Male; D051381:Rats; D012680:Sensitivity and Specificity; D013256:Steroids / Q000008:administration & dosage / Q000032:analysis* / Q000378:metabolism / Q000493:pharmacokinetics*; D053719:Tandem Mass Spectrometry; D013997:Time Factors
chemical_list: D045930:Anabolic Agents; D013256:Steroids