title: A 96-well format for a high-throughput baculovirus generation, fast titering and recombinant protein production in insect and mammalian cells.
abstract: BACKGROUND
  Baculovirus expression vector system (BEVS) has become a standard in recombinant protein production and virus-like particle preparation for numerous applications.
  
  
  RESULTS
  We describe here protocols which adapt baculovirus generation into 96-well format.
  
  
  CONCLUSIONS
  The established methodology allows simple baculovirus generation, fast virus titering within 18 h and efficient recombinant protein production in a high-throughput format. Furthermore, the produced baculovirus vectors are compatible with gene expression in vertebrate cells in vitro and in vivo.
authors.affiliation: Department of Biotechnology and Molecular Medicine, A.I. Virtanen Institute for Molecular Sciences, Kuopio, Finland. hanna-riikka.karkkainen@arktherapeutics.com
authors.forename: Hanna-Riikka
authors.initials: HR
authors.lastname: Kärkkäinen
authors.forename: Hanna P
authors.initials: HP
authors.lastname: Lesch
authors.forename: Antti I
authors.initials: AI
authors.lastname: Määttä
authors.forename: Pyry I
authors.initials: PI
authors.lastname: Toivanen
authors.forename: Anssi J
authors.initials: AJ
authors.lastname: Mähönen
authors.forename: Miia M
authors.initials: MM
authors.lastname: Roschier
authors.forename: Kari J
authors.initials: KJ
authors.lastname: Airenne
authors.forename: Olli H
authors.initials: OH
authors.lastname: Laitinen
authors.forename: Seppo
authors.initials: S
authors.lastname: Ylä-Herttuala