title: Development of a voltammetric technique for monitoring brain dopamine metabolism: compensation for interference caused by DOPAC electrogenerated during homovanillic acid detection.
abstract: The established stability of carbon-paste electrodes (CPEs) in brain extracellular fluid was exploited to develop a voltammetric technique to monitor the dopamine metabolite, homovanillic acid (HVA), at 10 s intervals. At the scan rates needed for this time resolution, 3,4-dihydroxyphenylacetic acid (DOPAC), electrogenerated as a result of HVA oxidation, was observed in the cyclic staircase voltammograms, and this interfered with the straightforward reliable quantification of HVA. However, correction of the HVA signal, recorded in mixtures, with currents from the DOPAC and ascorbate regions of the voltammogram allowed the reproducible construction of well behaved HVA calibration plots. These showed good linearity, LOD values, selectivity and stability during six days of continuous CPE exposure to a lipid medium, which served as an in-vitro model of CPE implantation in brain tissue for future applications.
authors.affiliation: UCD School of Chemistry and Chemical Biology, University College Dublin, Belfield, Dublin 4, Ireland.
authors.forename: Ismael A L
authors.initials: IA
authors.lastname: Mulla
authors.forename: John P
authors.initials: JP
authors.lastname: Lowry
authors.forename: Pier Andrea
authors.initials: PA
authors.lastname: Serra
authors.forename: Robert D
authors.initials: RD
authors.lastname: O'Neill
mesh_terms: D015102:3,4-Dihydroxyphenylacetic Acid / Q000737:chemistry*; D001205:Ascorbic Acid / Q000737:chemistry; D004298:Dopamine / Q000737:chemistry*; D055664:Electrochemical Techniques / Q000379:methods*; D006719:Homovanillic Acid / Q000737:chemistry*; D015394:Molecular Structure
chemical_list: D015102:3,4-Dihydroxyphenylacetic Acid; D001205:Ascorbic Acid; D004298:Dopamine; D006719:Homovanillic Acid