Spontaneous immortalization of oligodendroglial cells derived from an SV40 T antigen-positive human glioblastoma multiforme.
The polyoma group of viruses, including SV40, is known to be oncogenic in certain species. Here we report for the first time naturally occurring, immortalized tumor cells from a patient with glioblastoma multiforme (GBM); the cells were shown to be oligodendroglia; cells had developed remarkable chromosomal changes and were positive for SV40 T antigen. Therefore, we postulated that the main cause of immortalization of these cells was the expression of SV40 T antigen gene and protein. Since the cells are naturally generated, they will provide a useful model to study the function of oligodendroglial cells and the development of GBM.
Kim
Boe-Hyun
BH
Ilsong Institute of Life Science, Hallym University, Ilsong B/D 1605-4, Gwanyang-dong, Dongan-gu, Anyang, Gyeonggi-do 431-060, South Korea.
Song
Jun-Ho
JH
Jeon
Yong-Chul
YC
Jeong
Byung-Hoon
BH
Yun
Sook-Kyung
SK
Cho
Hyoun-Chan
HC
Carp
Richard I
RI
Kim
Yong-Sun
YS
D000952:Antigens, Polyomavirus Transforming / Q000235:genetics; D015153:Blotting, Western; D001932:Brain Neoplasms / Q000235:genetics / Q000821:virology*; D002461:Cell Line, Transformed; D002472:Cell Transformation, Viral / Q000235:genetics; D002869:Chromosome Aberrations; D005260:Female; D005909:Glioblastoma / Q000235:genetics / Q000821:virology*; D006801:Humans; D007150:Immunohistochemistry; D008875:Middle Aged; D009836:Oligodendroglia / Q000166:cytology* / Q000821:virology; D034741:RNA, Small Interfering; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014162:Transfection
D000952:Antigens, Polyomavirus Transforming; D034741:RNA, Small Interfering