title: Simultaneous determination of five coumarins in Angelicae dahuricae Radix by HPLC/UV and LC-ESI-MS/MS.
abstract: Rapid, simple and reliable HPLC/UV and LC-ESI-MS/MS methods for the simultaneous determination of five active coumarins of Angelicae dahuricae Radix, byakangelicol (1), oxypeucedanin (2), imperatorin (3), phellopterin (4) and isoimperatorin (5) were developed and validated. The separation condition for HPLC/UV was optimized using a Develosil RPAQUEOUS C(30) column using 70% acetonitrile in water as the mobile phase. This HPLC/UV method was successful for providing the baseline separation of the five coumarins with no interfering peaks detected in the 70% ethanol extract of Angelicae dahuricae Radix. The specific determination of the five coumarins was also accomplished by a triple quadrupole tandem mass spectrometer equipped with an electrospray ionization source (LC-ESI-MS/MS). Multiple reaction monitoring (MRM) in the positive mode was used to enhance the selectivity of detection. The LC-ESI-MS/MS methods were successfully applied for the determination of the five major coumarins in Angelicae dahuricae Radix. These HPLC/UV and LC-ESI-MS/MS methods were validated in terms of recovery, linearity, accuracy and precision (intra- and inter-day validation). Taken together, the shorter analysis time involved makes these HPLC/UV and LC-ESI-MS/MS methods valuable for the commercial quality control of Angelicae dahuricae Radix extracts and its pharmaceutical preparations.
authors.affiliation: Department of Pharmaceutical Analysis, College of Pharmacy, Chung-Ang University, 221 Heuksuk-Dong, Dongjak-Ku, Seoul 156-756, Korea.
authors.forename: Ah Yeon
authors.initials: AY
authors.lastname: Park
authors.forename: So-Young
authors.initials: SY
authors.lastname: Park
authors.forename: Jaehyun
authors.initials: J
authors.lastname: Lee
authors.forename: Mihye
authors.initials: M
authors.lastname: Jung
authors.forename: Jinwoong
authors.initials: J
authors.lastname: Kim
authors.forename: Sam Sik
authors.initials: SS
authors.lastname: Kang
authors.forename: Jeong-Rok
authors.initials: JR
authors.lastname: Youm
authors.forename: Sang Beom
authors.initials: SB
authors.lastname: Han
mesh_terms: D029969:Angelica / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003374:Coumarins / Q000032:analysis*; D005663:Furans / Q000032:analysis; D011564:Furocoumarins / Q000032:analysis; D016014:Linear Models; D018517:Plant Roots / Q000737:chemistry; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet / Q000379:methods; D053719:Tandem Mass Spectrometry / Q000379:methods*; D013696:Temperature
chemical_list: D003374:Coumarins; D005663:Furans; D011564:Furocoumarins; C055542:isoimperatorin; C104601:phellopterin; C462034:byakangelicol; C031534:imperatorin; C031535:oxypeucadanin