title: Systems biology coupled with label-free high-throughput detection as a novel approach for diagnosis of chronic obstructive pulmonary disease.
abstract: Chronic obstructive pulmonary disease (COPD) is a treatable and preventable disease state, characterised by progressive airflow limitation that is not fully reversible. Although COPD is primarily a disease of the lungs there is now an appreciation that many of the manifestations of disease are outside the lung, leading to the notion that COPD is a systemic disease. Currently, diagnosis of COPD relies on largely descriptive measures to enable classification, such as symptoms and lung function. Here the limitations of existing diagnostic strategies of COPD are discussed and systems biology approaches to diagnosis that build upon current molecular knowledge of the disease are described. These approaches rely on new 'label-free' sensing technologies, such as high-throughput surface plasmon resonance (SPR), that we also describe.
authors.affiliation: Cell Biophysics Group, School of Biology, The University of Nottingham, NG7 2RD, UK. joanna.richens@nottingham.ac.uk
authors.forename: Joanna L
authors.initials: JL
authors.lastname: Richens
authors.forename: Richard A
authors.initials: RA
authors.lastname: Urbanowicz
authors.forename: Elizabeth A M
authors.initials: EA
authors.lastname: Lunt
authors.forename: Rebecca
authors.initials: R
authors.lastname: Metcalf
authors.forename: Jonathan
authors.initials: J
authors.lastname: Corne
authors.forename: Lucy
authors.initials: L
authors.lastname: Fairclough
authors.forename: Paul
authors.initials: P
authors.lastname: O'Shea
mesh_terms: D000465:Algorithms; D015415:Biomarkers / Q000032:analysis; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D003936:Diagnosis, Computer-Assisted / Q000379:methods*; D020869:Gene Expression Profiling / Q000379:methods*; D006801:Humans; D020543:Proteome / Q000032:analysis*; D029424:Pulmonary Disease, Chronic Obstructive / Q000175:diagnosis* / Q000378:metabolism*; D013194:Staining and Labeling; D020349:Surface Plasmon Resonance / Q000295:instrumentation / Q000379:methods*; D049490:Systems Biology / Q000379:methods
chemical_list: D015415:Biomarkers; D020543:Proteome