Endoplasmic reticulum in the penumbra following middle cerebral artery occlusion in the rabbit.
Caspase-12 has been localized to endoplasmic reticulum (ER) and showed to involve ER stress-induced apoptosis. In the present work we investigated the temporospatial alterations of caspase-12 immunoreactivity in the penumbra following cerebral ischemia/reperfusion in rabbit. Transient cerebral ischemia was produced by intraluminal occlusion of the middle cerebral artery for 2 h followed by 1 h, 6 h, 1 day, 3 days, 7 days and 14 days of reperfusion. Caspase-12 immunohistochemistry was first increased in the penumbra 1 h after reperfusion, with a peak at day 1 to day 3, and then gradually decreased to basal level at day 14. The number of TUNEL-positive cells and ultrastructural observation of brain sections in the penumbra showed a similar change at the same time points. ER mediated by caspase-12 participated in apoptosis induced by cerebral ischemia/reperfusion injury, which may provide a new area for therapeutic intervention to ameliorate outcomes following cerebral ischemia.
Liu
Huai-Jun
HJ
Department of Medical Imaging, The Second Hospital of Hebei Medical University, 215 West Heping Road, Shijiazhuang, 050000, Hebei Province, China. huaijun_hb@yahoo.com.cn
Yang
Ji-Ping
JP
Wang
Cang-Hai
CH
Liu
Rui-Chun
RC
Li
Ying
Y
Li
Chun-Yan
CY
D000818:Animals; D017209:Apoptosis / Q000502:physiology*; D053454:Caspase 12 / Q000378:metabolism*; D054022:Cerebrum / Q000201:enzymology* / Q000473:pathology / Q000648:ultrastructure; D004721:Endoplasmic Reticulum / Q000201:enzymology*; D005500:Follow-Up Studies; D007150:Immunohistochemistry; D020244:Infarction, Middle Cerebral Artery / Q000150:complications / Q000201:enzymology / Q000473:pathology; D008297:Male; D011817:Rabbits; D015427:Reperfusion Injury / Q000201:enzymology* / Q000209:etiology / Q000473:pathology; D013997:Time Factors
D053454:Caspase 12