An optical coherence microscope for 3-dimensional imaging in developmental biology.
An optical coherence microscope (OCM) has been designed and constructed to acquire 3-dimensional images of highly scattering biological tissue. Volume-rendering software is used to enhance 3-D visualization of the data sets. Lateral resolution of the OCM is 5 mm (FWHM), and the depth resolution is 10 mm (FWHM) in tissue. The design trade-offs for a 3-D OCM are discussed, and the fundamental photon noise limitation is measured and compared with theory. A rotating 3-D image of a frog embryo is presented to illustrate the capabilities of the instrument.
Hoeling
B
B
Fernandez
A
A
Haskell
R
R
Huang
E
E
Myers
W
W
Petersen
D
D
Ungersma
S
S
Wang
R
R
Williams
M
M
Fraser
S
S