Low prevalence of drug resistance transmitted virus in HIV Type 1-infected ARV-naive patients in Cambodia.
Abstract Between November 2006 and June 2007, HIV-1 reverse transcriptase (RT) and protease (PR) genes of 67 ARV-naive Cambodian patients were amplified and sequenced. At inclusion, the median age and duration of HIV infection were 28 and 1.1 years, respectively. The median CD4 and HIV-1 RNA were 611 cells/ml [IQR: 525-759] and 4.0 log(10) copies/ml [IQR: 3.4-4.6]. Among 67 HIV-1 strains, 95.5% were CRF 01_AE viruses (n = 64) whereas three clustered with subtype B. RT analysis indicated that only 1 patient out of 67, presenting K103N and M184V mutations, was resistant to NVP/EFV and 3TC/FTC. No primary resistance to protease inhibitors was detected in 59 amplified protease genes. The 1.49% (IC 95%: 0.04-8.04%) prevalence of transmitted drug-resistant strains in drug-naive patients was low in our study. Surveys of drug-resistant transmitted viruses should be regularly performed regarding the increasing access to HAART in Cambodia.
Nouhin
Janin
J
HIV/Hepatitis Laboratory, Pasteur Institute of Cambodia, Phnom Penh, Cambodia.
Ngin
Sopheak
S
Martin
P Regis
PR
Marcy
Olivier
O
Kruy
Leangsim
L
Ariey
Frederic
F
Peeters
Martine
M
Chaix
Marie-Laure
ML
Ayouba
Ahidjo
A
Nerrienet
Eric
E
D000328:Adult; D019943:Amino Acid Substitution / Q000235:genetics; D018791:CD4 Lymphocyte Count; D002160:Cambodia; D024882:Drug Resistance, Viral; D005260:Female; D005838:Genotype; D015658:HIV Infections / Q000635:transmission / Q000821:virology*; D016333:HIV Protease / Q000235:genetics*; D054303:HIV Reverse Transcriptase / Q000235:genetics*; D015497:HIV-1 / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D020125:Mutation, Missense; D012367:RNA, Viral / Q000097:blood; D017422:Sequence Analysis, DNA; D019562:Viral Load
D012367:RNA, Viral; C514824:reverse transcriptase, Human immunodeficiency virus 1; D054303:HIV Reverse Transcriptase; D016333:HIV Protease; C514803:p16 protease, Human immunodeficiency virus 1