2DE for proteome analysis of human metaphase chromosomes.
Two-dimensional electrophoresis (2DE) is one of the most effective methods for the reliable separation of proteins in a single gel. In our proteome analyses of human chromosomes, we used two types of 2DE: two-dimensional isoelectric focusing SDS-polyacrylamide gel electrophoresis (2D IEF/SDS-PAGE) and radical-free and highly reduced two-dimensional polyacrylamide gel electrophoresis (RFHR 2D PAGE) together with one-dimensional SDS-polyacrylamide gel electrophoresis (1DE). Experimental details of these gel electrophoresis procedures that have been shown to be effective for human proteome analyses are described in detail.
Fukui
Kiichi
K
Department of Biotechnology, Laboratory of Dynamic Cell Biology, Graduate School of Engineering, Osaka University, Osaka, Japan.
Uchiyama
Susumu
S
D002460:Cell Line; D002877:Chromosomes, Human / Q000737:chemistry*; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods*; D006801:Humans; D008677:Metaphase / Q000235:genetics*; D020543:Proteome / Q000032:analysis*
D020543:Proteome