[Co-production of thrombolytic enzyme and gamma-polyglutamic acid by liquid-culture of Bacillus subtilis SBS].
OBJECTIVE
To analyze the co-production of thrombolytic enzyme and gamma-polyglutamic acid by liquid-culture of Bacillus subtilis SBS.


METHODS
We used Bacillus subtilis SBS to produce thrombolytic enzyme (BTE) and 7-polyglutamic acid (gamma-PGA) by liquid fermentation. We conducted orthogonal experiments analysis between carbon source and nitrogen source. Then through Fibrin-SDS-PAGE, UV spectrum, Infra-Red spectrum and high performance liquid chromatography (HPLC), we identified the production.


RESULTS
We synthesized gamma-PGA in the medium without the addition of L-glutamic acid. Bacillus subtilis SBS was a L-glutamic acid-independent bacterium. The suitable carbon and nitrogen sources for the synthesis of thrombolytic enzyme (BTE) were soluble starch and soybean peptone. However,the suitable carbon and nitrogen sources for the synthesis of gamma-PGA were sucrose and NH4Cl.


CONCLUSIONS
The yields of both products could close to the levels of the single synthesis when the concentrations of sucrose, soybean peptone and NH4Cl were 10, 20, 8 g/L respectively, the activity of BTE reached 265 +/- 25 IU/mL,and the concentration of gamma-PGA reached 1.183 +/- 0.015 g/L.
Hu
Chungang
C
TEDA BIO-X Center for Systems Biotechnology, College of Food Engineering and Biotechnology, Tianjin University of Science Technology, Tianjin 300457, China. huchungang27@163.com
Liu
Changjin
C
Zheng
Huanlan
H
Zhou
Ping
P
D001412:Bacillus subtilis / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D003470:Culture Media / Q000494:pharmacology*; D005343:Fibrinolytic Agents / Q000378:metabolism*; D011099:Polyglutamic Acid / Q000031:analogs & derivatives* / Q000096:biosynthesis
D003470:Culture Media; D005343:Fibrinolytic Agents; C511775:poly(gamma-glutamic acid); D011099:Polyglutamic Acid