title: [Effect of the drug-resistant attenuated Bacillus proteus on CD80 and CD86 protein expression in peripheral blood dendritic cells of hepatitis B patients].
abstract: OBJECTIVE
  To explore the effect of the drug-resistant attenuated Bacillus proteus on the protein expression of CD80 and CD86 in peripheral blood dendritic cells (DCs) of hepatitis B patients.
  
  
  METHODS
  Peripheral blood monocytes were isolated from HBV-infected patients and the DCs were separated and induced to differentiate in vitro. The expression of CD80 and CD86 proteins on cultured DCs were examined using flow cytometry.
  
  
  RESULTS
  The expression rate of CD80 and CD86 of chronic hepatitis B patients increased significantly (P=0.000), while the positive expression rate of CD80 and CD86 showed no obvious variation in healthy individuals (P=0.185 and P=0.118, respectively).
  
  
  CONCLUSIONS
  Bacillus proteus can significantly increase CD80- and CD86-positive cell percentage in peripheral blood DCs of HBV-infected patients, but has no such an effect in healthy individuals.
authors.affiliation: Jiangdu Hospital, Southern Medical University, Guangzhou 510450, China. wangpeng72 wp@163.com
authors.forename: Peng
authors.initials: P
authors.lastname: Wang
authors.forename: Si-yun
authors.initials: SY
authors.lastname: Shu
authors.forename: Yan-qun
authors.initials: YQ
authors.lastname: Hu
mesh_terms: D000293:Adolescent; D000328:Adult; D018122:B7-1 Antigen / Q000378:metabolism*; D051940:B7-2 Antigen / Q000378:metabolism*; D001407:Bacillus / Q000187:drug effects / Q000276:immunology*; D016022:Case-Control Studies; D002648:Child; D003713:Dendritic Cells / Q000276:immunology / Q000378:metabolism*; D024881:Drug Resistance, Bacterial; D005260:Female; D005786:Gene Expression Regulation / Q000276:immunology*; D006509:Hepatitis B / Q000276:immunology* / Q000378:metabolism; D006801:Humans; D008297:Male; D008875:Middle Aged; D014613:Vaccines, Attenuated / Q000276:immunology; D055815:Young Adult
chemical_list: D018122:B7-1 Antigen; D051940:B7-2 Antigen; D014613:Vaccines, Attenuated