title: Structure of TTHA1623, a novel metallo-beta-lactamase superfamily protein from Thermus thermophilus HB8.
abstract: TTHA1623 is a metallo-beta-lactamase superfamily protein from the extremely thermophilic bacterium Thermus thermophilus HB8. Homologues of TTHA1623 exist in a wide range of bacteria and archaea and one eukaryote, Giardia lamblia, but their function remains unknown. To analyze the structural properties of TTHA1623, the crystal structures of its iron-bound and zinc-bound forms have been determined to 2.8 and 2.2 A resolution, respectively. TTHA1623 possesses an alphabetabetaalpha-fold similar to that of other metallo-beta-lactamase superfamily proteins with glyoxalase II-type metal coordination. However, TTHA1623 exhibits a putative substrate-binding pocket with a unique shape.
authors.affiliation: Department of Applied Biological Chemistry, University of Tokyo, Yayoi, Bunkyo-ku, Japan.
authors.forename: Akihiro
authors.initials: A
authors.lastname: Yamamura
authors.forename: Akitoshi
authors.initials: A
authors.lastname: Okada
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Kameda
authors.forename: Jun
authors.initials: J
authors.lastname: Ohtsuka
authors.forename: Noriko
authors.initials: N
authors.lastname: Nakagawa
authors.forename: Akio
authors.initials: A
authors.lastname: Ebihara
authors.forename: Koji
authors.initials: K
authors.lastname: Nagata
authors.forename: Masaru
authors.initials: M
authors.lastname: Tanokura
mesh_terms: D018360:Crystallography, X-Ray; D008670:Metals / Q000737:chemistry* / Q000378:metabolism*; D008958:Models, Molecular; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D040681:Structural Homology, Protein; D016963:Thermus thermophilus / Q000201:enzymology* / Q000235:genetics; D001618:beta-Lactamases / Q000737:chemistry* / Q000145:classification / Q000235:genetics / Q000378:metabolism*
chemical_list: D008670:Metals; D001618:beta-Lactamases