title: Assembled DJ beta complexes influence TCR beta chain selection and peripheral V beta repertoire.
abstract: TCRbeta chain repertoire of peripheral alphabeta T cells is generated through the stepwise assembly and subsequent selection of TCRbeta V region exons during thymocyte development. To evaluate the influence of a two-step recombination process on Vbeta rearrangement and selection, we generated mice with a preassembled Dbeta1Jbeta1.1 complex on the Jbeta1(omega) allele, an endogenous TCRbeta allele that lacks the Dbeta2-Jbeta2 cluster, creating the Jbeta1(DJbeta) allele. As compared with Jbeta1(omega/omega) mice, both Jbeta1(DJbeta/omega) and Jbeta1(DJbeta/DJbeta) mice exhibited grossly normal thymocyte development and TCRbeta allelic exclusion. In addition, Vbeta rearrangements on Jbeta1(DJbeta) and Jbeta1(omega) alleles were similarly regulated by TCRbeta-mediated feedback regulation. However, in-frame VbetaDJbeta rearrangements were present at a higher level on the Jbeta1(DJbeta) alleles of Jbeta1(DJbeta/omega) alphabeta T cell hybridomas, as compared with on the Jbeta1(omega) alleles. This bias was most likely due to both an increased frequency of Vbeta-to-DJbeta rearrangements on Jbeta1(DJbeta) alleles and a preferential selection of cells with in-frame VbetaDJbeta exons assembled on Jbeta1(DJbeta) alleles during the development of Jbeta1(DJbeta/omega) alphabeta T cells. Consistent with the differential selection of in-frame VbetaDJbeta rearrangements on Jbeta1(DJbeta) alleles, the Vbeta repertoire of alphabeta T cells was significantly altered during alphabeta TCR selection in Jbeta1(DJbeta/omega) and Jbeta1(DJbeta/DJbeta) mice, as compared with in Jbeta1(omega/omega) mice. Our data indicate that the diversity of DJbeta complexes assembled during thymocyte development influences TCRbeta chain selection and peripheral Vbeta repertoire.
authors.affiliation: Immunology Graduate Group, University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA.
authors.forename: Andrea C
authors.initials: AC
authors.lastname: Carpenter
authors.forename: Katherine S
authors.initials: KS
authors.lastname: Yang-Iott
authors.forename: Linda H
authors.initials: LH
authors.lastname: Chao
authors.forename: Beth
authors.initials: B
authors.lastname: Nuskey
authors.forename: Scott
authors.initials: S
authors.lastname: Whitlow
authors.forename: Frederick W
authors.initials: FW
authors.lastname: Alt
authors.forename: Craig H
authors.initials: CH
authors.lastname: Bassing
mesh_terms: D000483:Alleles; D000818:Animals; D000916:Antibody Diversity / Q000235:genetics* / Q000276:immunology; D001483:Base Sequence; D045744:Cell Line, Tumor; D002478:Cells, Cultured; D015329:Gene Rearrangement, T-Lymphocyte / Q000276:immunology*; D018390:Gene Targeting; D007133:Immunoglobulin Joining Region / Q000096:biosynthesis / Q000235:genetics*; D007135:Immunoglobulin Variable Region / Q000096:biosynthesis / Q000235:genetics*; D051379:Mice; D008822:Mice, Transgenic; D008969:Molecular Sequence Data; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000096:biosynthesis / Q000235:genetics*; D011995:Recombination, Genetic; D016176:T-Lymphocyte Subsets / Q000166:cytology / Q000276:immunology* / Q000378:metabolism*
chemical_list: D007133:Immunoglobulin Joining Region; D007135:Immunoglobulin Variable Region; D016693:Receptors, Antigen, T-Cell, alpha-beta