pH-dependent surface-enhanced resonance Raman scattering of yeast iso-1-cytochrome c adsorbed on silver nanoparticle surfaces under denaturing conditions at pH<3.
We measured the pH-induced spectral changes of yeast iso-1-cytochrome c on silver nanoparticle surfaces using surface-enhanced resonance Raman scattering (SERRS) at 457.9 nm. At a pH of 3, the Met80 ligand in yeast iso-1-cytochrome c is assumed to dissociate, leading to a marked conformational change as evidenced by the vibrational spectral shifts. The Soret band at 410 nm in the UV-Vis spectrum shifted to 396 nm at pH 3, indicating a transition from a low spin state to a high spin state from a weak interaction with a water molecule. Thus, SERRS spectroscopy can measure the pH-induced denaturalization of cyt c adsorbed on metal nanoparticle surfaces at a lower concentration with a better sensitivity than ordinary resonance Raman spectroscopy.
Lee
So Yeong
SY
Department of Pharmacology, College of Veterinary Medicine, Seoul National University, Seoul, Korea.
Joo
Sang-Woo
SW
Lee
Seonghoon
S
Lim
Manho
M
D045304:Cytochromes c / Q000737:chemistry*; D053758:Nanoparticles / Q000737:chemistry*; D011489:Protein Denaturation; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry*; D012834:Silver / Q000737:chemistry*; D013059:Spectrum Analysis, Raman / Q000379:methods*
C014059:CYC1 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D012834:Silver; D045304:Cytochromes c