title: Characterization of the Corynebacterium glutamicum deltapimB' deltamgtA double deletion mutant and the role of Mycobacterium tuberculosis orthologues Rv2188c and Rv0557 in glycolipid biosynthesis.
abstract: In this study, utilizing a Corynebacterium glutamicum DeltapimB' DeltamgtA double deletion mutant, we unequivocally assign the in vivo functions of Rv2188c as an Ac(1)PIM(1):mannosyltransferase (originally termed PimB'(Mt) [Mycobacterium tuberculosis PimB']) and Rv0557 as a GlcAGroAc(2):mannosyltransferase (originally termed PimB(Mt)), which we have reassigned as PimB(Mt) and MgtA(Mt), respectively, in Mycobacterium tuberculosis.
authors.affiliation: School of Biosciences, University of Birmingham, Edgbaston, Birmingham, United Kingdom.
authors.forename: Arun K
authors.initials: AK
authors.lastname: Mishra
authors.forename: Sarah
authors.initials: S
authors.lastname: Batt
authors.forename: Karin
authors.initials: K
authors.lastname: Krumbach
authors.forename: Lothar
authors.initials: L
authors.lastname: Eggeling
authors.forename: Gurdyal S
authors.initials: GS
authors.lastname: Besra
mesh_terms: D000251:Adenosine Triphosphatases / Q000235:genetics / Q000378:metabolism*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D048230:Corynebacterium glutamicum / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism; D004287:Dolichol Monophosphate Mannose / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial; D005816:Genetic Complementation Test; D006017:Glycolipids / Q000096:biosynthesis* / Q000378:metabolism; D008070:Lipopolysaccharides / Q000378:metabolism; D008364:Mannosyltransferases / Q000235:genetics / Q000378:metabolism*; D026901:Membrane Transport Proteins / Q000235:genetics / Q000378:metabolism*; D008954:Models, Biological; D017384:Sequence Deletion / Q000235:genetics
chemical_list: D001426:Bacterial Proteins; C480221:Cfp32 protein, Mycobacterium tuberculosis; D006017:Glycolipids; D008070:Lipopolysaccharides; D026901:Membrane Transport Proteins; D004287:Dolichol Monophosphate Mannose; D008364:Mannosyltransferases; C400532:PimB protein, Mycobacterium tuberculosis; D000251:Adenosine Triphosphatases; C093585:MgtA protein, bacteria