Systems approach to therapeutics design.
A general methodology is described for improving the therapeutic properties of protein drugs by engineering novel intracellular trafficking pathways. Procedures for cellular trafficking experiments and mathematical modeling of trafficking pathways are presented. Previous work on the engineering of the transferrin trafficking pathway will be used to illustrate how each step of the methodology can be applied.
Lao
Bert J
BJ
Department of Bioengineering, University of California, Los Angeles, USA.
Kamei
Daniel T
DT
D003198:Computer Simulation; D016503:Drug Delivery Systems / Q000379:methods*; D006367:HeLa Cells; D006801:Humans; D015202:Protein Engineering; D021381:Protein Transport; D011506:Proteins / Q000627:therapeutic use*; D049490:Systems Biology / Q000379:methods*; D014168:Transferrin / Q000378:metabolism*
D011506:Proteins; D014168:Transferrin