Filimonas lacunae gen. nov., sp. nov., a member of the phylum Bacteroidetes isolated from fresh water.
A Gram-negative, strictly aerobic, motile, filamentous and viscous exopolymer-producing bacterium, designated strain YT21(T), was isolated from fresh water. Phylogenetic analysis of the 16S rRNA gene sequence indicated that strain YT21(T) lies within a cluster containing the established genera Segetibacter, Terrimonas, Niastella and Chitinophaga in the phylum Bacteroidetes. However, the isolate represented a lineage distinct from these genera, with sequence similarities ranging from 88.9 to 91.8 %. The genomic G+C content was 45.2 mol%. The predominant isoprenoid quinone was MK-7. The major fatty acids were 15 : 0 iso, 17 : 0 iso 3-OH and 15 : 1 iso G. On the basis of morphological features and phenotypic and phylogenetic data, strain YT21(T) represents a novel genus and species, for which the name Filimonas lacunae gen. nov., sp. nov. is proposed. The type strain of Filimonas lacunae is strain YT21(T) (=NBRC 104114(T) =DSM 21054(T)).
Shiratori
Hatsumi
H
Life Science Research Center, College of Bioresource Sciences, Nihon University, 1866 Kameino, Fujisawa 252-8510, Japan. siratori@brs.nihon-u.ac.jp
Tagami
Yudai
Y
Morishita
Tomoyo
T
Kamihara
Yuka
Y
Beppu
Teruhiko
T
Ueda
Kenji
K
D015373:Bacterial Typing Techniques; D041963:Bacteroidetes / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology; D001482:Base Composition; D004269:DNA, Bacterial / Q000032:analysis; D004275:DNA, Ribosomal / Q000032:analysis; D005227:Fatty Acids / Q000032:analysis; D005618:Fresh Water / Q000382:microbiology*; D020459:Genes, rRNA; D008969:Molecular Sequence Data; D010641:Phenotype; D010802:Phylogeny; D011809:Quinones / Q000032:analysis; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013045:Species Specificity
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D011809:Quinones; D012336:RNA, Ribosomal, 16S