title: Coagulation factor XIII serves as protein disulfide isomerase.
abstract: Tissue transglutaminase was reported to act as protein disulfide isomerase (PDI). We studied whether plasma transglutaminase - coagulation factor XIII (FXIII) - has PDI activity as well. PDI activity was measured by determining the ability to renature reduced-denatured RNase (rdRNase). We found that FXIII can renature rdRNase, with efficiency comparable to commercial PDI. This PDI activity was inhibited by bacitracin. Like tissue transglutaminase, FXIII-mediated PDI activity is independent of its transglutaminase activity and is located on the A subunit. Surface-associated PDI has been previously shown to catalyse two distinct functions: transnitrosation with subsequent release of intracellular nitric oxide and disulfide bond rearrangement during platelet integrin ligation. Our results imply that FXIII-PDI activity may have a role in platelet function.
authors.affiliation: Thrombosis and Hemostasis Unit, Beilinson Hospital, Rabin Medical Center, Petah Tikva, Israel.
authors.forename: Judith
authors.initials: J
authors.lastname: Lahav
authors.forename: Eli
authors.initials: E
authors.lastname: Karniel
authors.forename: Zsuzsa
authors.initials: Z
authors.lastname: Bagoly
authors.forename: Vera
authors.initials: V
authors.lastname: Sheptovitsky
authors.forename: Rima
authors.initials: R
authors.lastname: Dardik
authors.forename: Aida
authors.initials: A
authors.lastname: Inbal
mesh_terms: D000818:Animals; D000906:Antibodies; D001414:Bacitracin / Q000494:pharmacology; D002417:Cattle; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005176:Factor XIII / Q000037:antagonists & inhibitors / Q000737:chemistry / Q000276:immunology / Q000378:metabolism*; D026122:Factor XIIIa / Q000037:antagonists & inhibitors / Q000737:chemistry / Q000276:immunology / Q000378:metabolism*; D006801:Humans; D019704:Protein Disulfide-Isomerases / Q000037:antagonists & inhibitors / Q000097:blood* / Q000737:chemistry / Q000276:immunology; D020673:Protein Renaturation; D021122:Protein Subunits; D012259:Ribonuclease, Pancreatic / Q000378:metabolism*
chemical_list: D000906:Antibodies; D004791:Enzyme Inhibitors; D021122:Protein Subunits; D001414:Bacitracin; D005176:Factor XIII; D026122:Factor XIIIa; D012259:Ribonuclease, Pancreatic; D019704:Protein Disulfide-Isomerases