Expression patterns of 5-HT receptor subtypes 1A and 2A on GABAergic neurons within the spinal dorsal horn of GAD67-GFP knock-in mice.
5-HT plays an important role in GABA transmission at the spinal cord level. The main purpose of the present article is to find out, which 5-HT receptor subtypes may be involved in the regulation of GABAergic transmission in the spinal dorsal horn, by using the combination of tissue RT-PCR, single-cell RT-PCR and double immunofluorescent staining on the GAD(67)-GFP knock-in mice. The present result showed that 5-HT(1A) receptor subtype mRNA was present on about half of GFP-positive GABAergic interneurons in the spinal dorsal horn, which suggests a role of 5-HT(1A) receptor in GABA-mediated modulation of nociception at the spinal cord level. However, it is unexpected that just a very limited sub-population of the total GABAergic interneurons express 5-HT(2A) receptor mRNA or proteins in the spinal dorsal horn, which suggests that the 5-HT(2A) receptor subtype may not be important for spinal GABAergic effects in nociceptive modulation.
Wang
Ya-Yun
YY
Department of Anatomy and K.K. Leung Brain Research Centre, The Fourth Military Medical University, Xi'an 710032, People's Republic of China.
Wei
Yan-Yan
YY
Huang
Jing
J
Wang
Wei
W
Tamamaki
Nobuaiki
N
Li
Yun-Qing
YQ
Wu
Sheng-Xi
SX
D000818:Animals; D005260:Female; D005455:Fluorescent Antibody Technique; D005786:Gene Expression Regulation / Q000502:physiology; D055879:Gene Knock-In Techniques; D005968:Glutamate Decarboxylase / Q000235:genetics*; D049452:Green Fluorescent Proteins / Q000235:genetics; D007395:Interneurons / Q000166:cytology / Q000378:metabolism*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D009433:Neural Inhibition / Q000235:genetics; D009619:Nociceptors / Q000166:cytology / Q000378:metabolism; D010146:Pain / Q000235:genetics / Q000378:metabolism / Q000503:physiopathology; D020671:Posterior Horn Cells / Q000166:cytology / Q000378:metabolism*; D044282:Receptor, Serotonin, 5-HT1A / Q000235:genetics / Q000378:metabolism*; D044402:Receptor, Serotonin, 5-HT2A / Q000235:genetics / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012701:Serotonin / Q000378:metabolism; D009435:Synaptic Transmission / Q000235:genetics; D005680:gamma-Aminobutyric Acid / Q000378:metabolism*
D044402:Receptor, Serotonin, 5-HT2A; D011993:Recombinant Fusion Proteins; D044282:Receptor, Serotonin, 5-HT1A; D049452:Green Fluorescent Proteins; D012701:Serotonin; D005680:gamma-Aminobutyric Acid; D005968:Glutamate Decarboxylase; C402565:glutamate decarboxylase 1