Effect of increasing dialyzer mass transfer area coefficient and dialysate flow on clearance of protein-bound solutes: a pilot crossover trial.
BACKGROUND
Protein-bound solutes are poorly cleared by means of conventional hemodialysis because protein binding limits the "free" solute concentration driving diffusion. This study tested the modeled prediction that clearances of bound solutes could be increased by increasing the dialyzer mass transfer area coefficient (K(o)A) and dialysate flow (Q(d)) to greater than the levels used in conventional practice.


METHODS
Pilot crossover trial.


METHODS
6 stable long-term hemodialysis patients.


METHODS
Study participants underwent an experimental dialysis treatment in which K(o)A and Q(d) were increased by using 2 dialyzers in series and supplying each dialyzer with a Q(d) of 800 mL/min by using 2 dialysis machines. Experimental clearances were compared with those during a conventional treatment with a single dialyzer and Q(d) of 800 mL/min supplied by 1 machine.


RESULTS
Measured clearances of uremic solutes.


METHODS
Clearances were measured for urea nitrogen and the bound solutes p-cresol sulfate, indoxyl sulfate, kynurenic acid, and hippurate.


RESULTS
Clearances for the bound solutes during conventional treatment were lower than for urea nitrogen (clearance values: urea nitrogen, 255 +/- 16 mL/min; p-cresol sulfate, 23 +/- 4 mL/min; indoxyl sulfate, 30 +/- 7 mL/min; kynurenic acid, 43 +/- 4 mL/min; and hippurate, 115 +/- 11 mL/min). Experimental treatment increased clearances of all solutes (clearance values: urea nitrogen, 318 +/- 19 mL/min; p-cresol sulfate, 37 +/- 6 mL/min; indoxyl sulfate, 46 +/- 8 mL/min; kynurenic acid, 73 +/- 7 mL/min; and hippurate, 165 +/- 17 mL/min). The magnitude of the increases in clearance was greater for bound solutes than for urea nitrogen (increase in clearance: urea nitrogen, 25% +/- 6%; p-cresol sulfate, 66% +/- 19%; indoxyl sulfate, 57% +/- 27%; kynurenic acid, 69% +/- 5%; and hippurate, 44% +/- 15%).


CONCLUSIONS
A longer term study would be required to determine whether increased dialytic clearance of bound solutes leads to a decrease in plasma solute levels.


CONCLUSIONS
Dialytic clearance of protein-bound solutes can be increased by increasing K(o)A and Q(d) to greater than conventional levels.
Luo
Frank J-G
FJ
Department of Medicine, VA Palo Alto HCS and Stanford University, Palo Alto, CA 94303, USA. frankluo_za@yahoo.com
Patel
Kajal P
KP
Marquez
Ilian O
IO
Plummer
Natalie S
NS
Hostetter
Thomas H
TH
Meyer
Timothy W
TW
D001806:Blood Urea Nitrogen; D018592:Cross-Over Studies; D015314:Dialysis Solutions / Q000008:administration & dosage* / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D007674:Kidney Diseases / Q000097:blood / Q000628:therapy; D007736:Kynurenic Acid / Q000097:blood / Q000378:metabolism; D008657:Metabolic Clearance Rate / Q000502:physiology; D010865:Pilot Projects; D011485:Protein Binding / Q000502:physiology; D011506:Proteins / Q000378:metabolism*; D006435:Renal Dialysis / Q000295:instrumentation* / Q000379:methods*
D015314:Dialysis Solutions; D011506:Proteins; D007736:Kynurenic Acid