In vitro and in vivo antitrypanosomal activities of three peptide antibiotics: leucinostatin A and B, alamethicin I and tsushimycin.
In the course of our screening for antitrypanosomal compounds from soil microorganisms, as well as from the antibiotics library of the Kitasato Institute for Life Sciences, we found three peptide antibiotics, leucinostatin (A and B), alamethicin I and tsushimycin, which exhibited potent or moderate antitrypanosomal activity. We report here the in vitro and in vivo antitrypanosomal properties and cytotoxicities of leucinostatin A and B, alamethicin I and tsushimycin compared with suramin. We also discuss their possible mode of action. This is the first report of in vitro and in vivo trypanocidal activity of leucinostatin A and B, alamethicin I and tsushimycin.
Ishiyama
Aki
A
Research Center for Tropical Diseases, Center for Basic Research, Kitasato Institute for Life Sciences and Graduate School of Infectious Control Sciences, Kitasato University, Tokyo, Japan.
Otoguro
Kazuhiko
K
Iwatsuki
Masato
M
Iwatsuki
Masahito
M
Namatame
Miyuki
M
Nishihara
Aki
A
Nonaka
Kenichi
K
Kinoshita
Yuta
Y
Takahashi
Yoko
Y
Masuma
Rokuro
R
Shiomi
Kazuro
K
Yamada
Haruki
H
Omura
Satoshi
S
D000408:Alamethicin / Q000302:isolation & purification / Q000494:pharmacology* / Q000627:therapeutic use; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology* / Q000627:therapeutic use; D023181:Antimicrobial Cationic Peptides; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D005260:Female; D005285:Fermentation; D055666:Lipopeptides / Q000302:isolation & purification / Q000494:pharmacology* / Q000627:therapeutic use; D051379:Mice; D008813:Mice, Inbred ICR; D010143:Paecilomyces / Q000145:classification / Q000378:metabolism*; D010455:Peptides / Q000302:isolation & purification / Q000494:pharmacology* / Q000627:therapeutic use; D010456:Peptides, Cyclic; D013498:Suramin / Q000494:pharmacology; D014344:Trypanocidal Agents; D014346:Trypanosoma brucei brucei / Q000187:drug effects; D016833:Trypanosoma brucei rhodesiense / Q000187:drug effects; D014353:Trypanosomiasis, African / Q000188:drug therapy* / Q000469:parasitology
D000900:Anti-Bacterial Agents; D023181:Antimicrobial Cationic Peptides; D055666:Lipopeptides; D010455:Peptides; D010456:Peptides, Cyclic; D014344:Trypanocidal Agents; C031299:tsushimycin; D000408:Alamethicin; D013498:Suramin; C008268:leucinostatin A; C040316:leucinostatin B