Increased phagocytosis of platelets from patients with secondary dengue virus infection by human macrophages.
The relationship between the percent phagocytosis of platelets by differentiated THP-1 cells was examined using flowcytometry and the peripheral platelet counts as well as platelet-associated IgG (PAIgG) in 36 patients with secondary dengue virus (DV) infections. The percent phagocytosis and the levels of PAIgG were significantly increased in these patients during the acute phase compared with the healthy volunteers. The increased percent phagocytosis and PAIgG found during the acute phase significantly decreased during the convalescent phase. An inverse correlation between platelet count and the percent phagocytosis (P = 0.011) and the levels of PAIgG (P = 0.041) was found among these patients during the acute phase. No correlation was found, however, between the percent phagocytosis and the levels of PAIgG. Our present data suggest that accelerated platelet phagocytosis occurs during the acute phase of secondary DV infections, and it is one of the mechanisms of thrombocytopenia in this disease.
Honda
Shoko
S
Department of Clinical Medicine and Virology, Institute of Tropical Medicine Nagasaki University, Japan.
Saito
Mariko
M
Dimaano
Efren M
EM
Morales
Philip A
PA
Alonzo
Maria T G
MT
Suarez
Lady-Anne C
LA
Koike
Natsuki
N
Inoue
Shingo
S
Kumatori
Atsushi
A
Matias
Ronald R
RR
Natividad
Filipinas F
FF
Oishi
Kazunori
K
D000293:Adolescent; D000328:Adult; D001792:Blood Platelets / Q000276:immunology*; D016022:Case-Control Studies; D003715:Dengue / Q000097:blood / Q000276:immunology*; D005434:Flow Cytometry; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D008264:Macrophages / Q000502:physiology*; D010587:Phagocytosis / Q000502:physiology*; D010976:Platelet Count; D055815:Young Adult
D007074:Immunoglobulin G