Immunohistochemical stains in Mohs surgery: a review.
BACKGROUND
During Mohs surgery, there are instances in which residual tumor cells may be difficult to detect, thereby increasing the risk of incomplete excision and tumor recurrence. It is possible to employ immunohistochemical techniques as an adjunct to routine hematoxylin and eosin staining to aid in ensuring negative margins.


OBJECTIVE
To review the literature regarding the use of immunostains in Mohs surgery.


RESULTS
Various immunostains have proved useful in detecting tumor cells in various malignancies, including melanoma, basal cell carcinoma, squamous cell carcinoma, dermatofibrosarcoma protuberans, extramammary Paget's disease, primary cutaneous mucinous carcinoma, granular cell tumor, and trichilemmal carcinoma.


CONCLUSIONS
In this article, we review immunohistochemical stains that have been employed in Mohs micrographic surgery and evaluate their utility in enhancing detection of residual tumors with respect to tumor type, particularly in situations in which detection of residual tumor may be difficult.
Stranahan
Donald
D
Department of Dermatology and Cutaneous Surgery, University of South Florida, Tampa, Florida, USA.
Cherpelis
Basil S
BS
Glass
L Frank
LF
Ladd
Sharron
S
Fenske
Neil A
NA
D000906:Antibodies; D004396:Coloring Agents; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods*; D015580:Mohs Surgery; D018365:Neoplasm, Residual / Q000473:pathology* / Q000601:surgery; D012878:Skin Neoplasms / Q000473:pathology* / Q000601:surgery
D000906:Antibodies; D004396:Coloring Agents