title: A candidate modified-live bovine coronavirus vaccine: safety and immunogenicity evaluation.
abstract: A modified-live vaccine against the respiratory form of bovine coronavirus (BCoV) infection was developed by progressive attenuation of a respiratory strain (438/06-TN). The vaccine was found to be safe as four colostrum-deprived newborn calves remained healthy after oronasal administration of ten doses of the vaccine. The immunogenicity of the vaccine was assessed by intramuscular injection of one vaccine dose to 30 BCoV-antibody negative 2-3-month-old calves. At 30 days post-vaccination, all vaccinated calves displayed high antibody titres against BCoV. Sequence analysis of the S gene of wild-type and cell-adapted 438/06-TN strain detected 10 nucleotide changes, 9 of which were non-synonymous.
authors.affiliation: Department of Public Health and Animal Sciences, Faculty of Veterinary Medicine of Bari, Strada per Casamassima km 3, 70010 Valenzano, Bari, Italy. n.decaro@veterinaria.uniba.it
authors.forename: Nicola
authors.initials: N
authors.lastname: Decaro
authors.forename: Marco
authors.initials: M
authors.lastname: Campolo
authors.forename: Viviana
authors.initials: V
authors.lastname: Mari
authors.forename: Costantina
authors.initials: C
authors.lastname: Desario
authors.forename: Maria Loredana
authors.initials: ML
authors.lastname: Colaianni
authors.forename: Livia
authors.initials: L
authors.lastname: Di Trani
authors.forename: Paolo
authors.initials: P
authors.lastname: Cordioli
authors.forename: Canio
authors.initials: C
authors.lastname: Buonavoglia
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D002417:Cattle; D002418:Cattle Diseases / Q000517:prevention & control* / Q000821:virology; D002460:Cell Line; D018352:Coronavirus Infections / Q000517:prevention & control / Q000662:veterinary* / Q000821:virology; D017938:Coronavirus, Bovine / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification; D006801:Humans; D008562:Membrane Glycoproteins / Q000235:genetics / Q000276:immunology; D008969:Molecular Sequence Data; D012141:Respiratory Tract Infections / Q000517:prevention & control / Q000662:veterinary / Q000821:virology; D017422:Sequence Analysis, DNA; D064370:Spike Glycoprotein, Coronavirus; D014611:Vaccination / Q000662:veterinary; D014613:Vaccines, Attenuated / Q000008:administration & dosage / Q000009:adverse effects / Q000235:genetics / Q000276:immunology; D014759:Viral Envelope Proteins / Q000235:genetics / Q000276:immunology; D014765:Viral Vaccines / Q000008:administration & dosage / Q000009:adverse effects / Q000235:genetics / Q000276:immunology
chemical_list: D000914:Antibodies, Viral; D008562:Membrane Glycoproteins; D064370:Spike Glycoprotein, Coronavirus; D014613:Vaccines, Attenuated; D014759:Viral Envelope Proteins; D014765:Viral Vaccines