title: Profiling histidine dipeptides in plasma and urine after ingesting beef, chicken or chicken broth in humans.
abstract: The in vitro metabolic stability of histidine-dipeptides (HD), carnosine (CAR) and anserine (ANS), in human serum, and their absorption kinetics after ingesting pure carnosine or HD rich foods in humans have been investigated. Healthy women (n = 4) went through four phases of taking one dose of either 450 mg of pure carnosine, 150 g beef (B), 150 g chicken (C), or chicken broth (CB) from 150 g chicken with a >2-week washout period between each phase. Blood samples were collected at 0, 30, 60, 100, 180, 240, and 300 min, and urine samples before and after (up to 7 h) ingesting pure carnosine or food. Both plasma and urine samples were analyzed for HD concentrations using a sensitive and selective LC-ESI-MS/MS method. CAR was undetectable in plasma after ingesting pure carnosine, B, C or CB. By contrast, plasma ANS concentration was significantly increased (P < 0.05) after ingesting C or CB, respectively. Urinary concentrations of both CAR and ANS were 13- to 14-fold increased after ingesting B, and 14.8- and 243-fold after CB ingestion, respectively. Thus, dietary HD, which are rapidly hydrolyzed by carnosinase in plasma, and excreted in urine, may act as reactive carbonyl species sequestering agents.
authors.affiliation: Jean Mayer USDA, Human Nutrition Research Center on Aging, Tufts University, 711 Washington St., Boston, MA 02111, USA. kyungjin.yeum@tufts.edu
authors.forename: Kyung-Jin
authors.initials: KJ
authors.lastname: Yeum
authors.forename: Marica
authors.initials: M
authors.lastname: Orioli
authors.forename: Luca
authors.initials: L
authors.lastname: Regazzoni
authors.forename: Marina
authors.initials: M
authors.lastname: Carini
authors.forename: Helen
authors.initials: H
authors.lastname: Rasmussen
authors.forename: Robert M
authors.initials: RM
authors.lastname: Russell
authors.forename: Giancarlo
authors.initials: G
authors.lastname: Aldini
mesh_terms: D000328:Adult; D000818:Animals; D000861:Anserine / Q000097:blood* / Q000378:metabolism / Q000652:urine*; D002336:Carnosine / Q000008:administration & dosage / Q000031:analogs & derivatives / Q000097:blood* / Q000378:metabolism / Q000652:urine*; D002417:Cattle; D002645:Chickens; D002851:Chromatography, High Pressure Liquid; D005260:Female; D006801:Humans; D007700:Kinetics; D008168:Lung / Q000378:metabolism; D008297:Male; D008460:Meat; D008875:Middle Aged; D011202:Poultry Products; D051381:Rats; D017208:Rats, Wistar; D053719:Tandem Mass Spectrometry; D015091:beta-Alanine / Q000097:blood
chemical_list: C033681:N-acetylcarnosine; D015091:beta-Alanine; D002336:Carnosine; D000861:Anserine