Frequency of human toxocariasis in a rural population from Cajamarca, Peru determined by DOT-ELISA test.
The aim of this study was to estimate the frequency of human toxocariasis in Cauday district, Cajamarca, Peru, using a dot-ELISA test. From June to October 2005, a total of 256 adult subjects were studied. Blood samples were collected for serology by a dot-ELISA test and for hematological examination. Parasitological examination was also carried out in stool samples to check cross-reactions in the dot-ELISA. The frequency observed was 44.92%, with a significant higher proportion of positivity in male subjects. From subjects with positive serology, 45.6% had respiratory symptoms, 40.44% abdominal pain, 32.35% hepatic symptoms, 14.7% cutaneous signs, 13.23% ocular manifestations, 43.38% eosinophilia, and all of these were statistically associated to serology. Among the population evaluated, 90.23% (231/256) were parasitized. From subjects with positive serology, 92.17% had at least one intestinal parasite and the most frequent were: Blastocystis hominis (68.38%), Giardia lamblia (28.68%), Hymenolepis nana (20.0%), Ascaris lumbricoides (15.65%), Entamoeba histolytica/E. dispar (13.24%), Cyclospora cayetanensis (4.41%), Cryptosporidium sp. (1.47%), Enterobius vermicularis (0.87%), Strongyloides stercoralis (0.87%), Taenia sp. (0.87%), and Trichuris trichiura (0.87%). The rate of false positives in the dot-ELISA test was improved by serum absorption each with A. suum antigens, with a decrease of cross-reactions. In conclusion, human toxocariasis is highly frequent in this population and some risk factors like dog/cat ownership, presence of pets within house, and previous history of geophagia were observed in the present study.
Roldán
William H
WH
Sección de Parasitología, Instituto de Medicina Tropical Daniel A. Carrión, Facultad de Medicina, Universidad Nacional Mayor de San Marcos, Callao, Perú. willyroldan23@hotmail.com
Espinoza
Yrma A
YA
Huapaya
Pedro E
PE
Huiza
Alina F
AF
Sevilla
Carlos R
CR
Jiménez
Susana
S
D000293:Adolescent; D000328:Adult; D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood*; D002415:Cats; D004285:Dogs; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood*; D007411:Intestinal Diseases, Parasitic / Q000175:diagnosis / Q000453:epidemiology* / Q000469:parasitology; D008297:Male; D008875:Middle Aged; D010568:Peru / Q000453:epidemiology; D015995:Prevalence; D012307:Risk Factors; D014119:Toxocara / Q000276:immunology* / Q000302:isolation & purification; D014120:Toxocariasis / Q000175:diagnosis / Q000453:epidemiology*; D055815:Young Adult
D000909:Antibodies, Helminth; D007074:Immunoglobulin G