Expression, purification, and characterization of exo-beta-D-glucosaminidase of Aspergillus sp. CJ22-326 from Escherichia coli.
An exo-beta-D-glucosaminidase gene was cloned from Aspergillus sp. CJ22-326 and expressed in Escherichia coli. The purified protein showed an exo-chitosanase activity in a viscosimetric assay and TLC analysis. This is the first report on cloning of a gene encoding an Aspergillus sp. exo-beta-D-glucosaminidase.
Li
Songlin
S
State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu, PR China.
Wang
Chen
C
Xia
Wenshui
W
D001230:Aspergillus / Q000201:enzymology*; D003001:Cloning, Molecular / Q000379:methods*; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D006596:Hexosaminidases / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*
D006596:Hexosaminidases; C064499:exo-beta-D-glucosaminidase