Ultra low-dose radiation: stress responses and impacts using rice as a grass model.
We report molecular changes in leaves of rice plants (Oryza sativa L. - reference crop plant and grass model) exposed to ultra low-dose ionizing radiation, first using contaminated soil from the exclusion zone around Chernobyl reactor site. Results revealed induction of stress-related marker genes (Northern blot) and secondary metabolites (LC-MS/MS) in irradiated leaf segments over appropriate control. Second, employing the same in vitro model system, we replicated results of the first experiment using in-house fabricated sources of ultra low-dose gamma (gamma) rays and selected marker genes by RT-PCR. Results suggest the usefulness of the rice model in studying ultra low-dose radiation response/s.
Rakwal
Randeep
R
Health Technology Research Center, National Institute of Advanced Industrial Science and Technology (AIST), West, Tsukuba, Ibaraki 305-8569, Japan.|Research Laboratory for Biotechnology and Biochemistry (RLABB), GPO Box 8207, Kathmandu, Nepal.
Agrawal
Ganesh Kumar
GK
Research Laboratory for Biotechnology and Biochemistry (RLABB), GPO Box 8207, Kathmandu, Nepal.
Shibato
Junko
J
Health Technology Research Center, National Institute of Advanced Industrial Science and Technology (AIST), West, Tsukuba, Ibaraki 305-8569, Japan.
Imanaka
Tetsuji
T
Research Reactor Institute, Kyoto University (KURRI), Osaka, Japan.
Fukutani
Satoshi
S
Research Reactor Institute, Kyoto University (KURRI), Osaka, Japan.
Tamogami
Shigeru
S
Laboratory of Growth Regulation Chemistry, Akita Prefectural University, Akita 010-0195, Japan.
Endo
Satoru
S
Graduate School of Engineering, Hiroshima University (HU), Hiroshima, Japan.
Sahoo
Sarata Kumar Sahoo
SKS
Research Center for Radiation Protection, National Institute of Radiological Sciences (NIRS), Chiba, Takoma Park, Maryland, USA.
Masuo
Yoshinori
Y
Health Technology Research Center, National Institute of Advanced Industrial Science and Technology (AIST), West, Tsukuba, Ibaraki 305-8569, Japan.
Kimura
Shinzo
S
Department of Research Planning and Coordination, Japan NIOSH, Kawasaki, Japan.
D005720:Gamma Rays; D008954:Models, Biological; D012275:Oryza / Q000378:metabolism / Q000528:radiation effects*; D018515:Plant Leaves / Q000378:metabolism / Q000528:radiation effects; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012717:Sesquiterpenes / Q000737:chemistry / Q000378:metabolism; D012987:Soil / Q000737:chemistry; D013312:Stress, Physiological / Q000528:radiation effects; D000094162:Phytoalexins
D010940:Plant Proteins; D012333:RNA, Messenger; D012717:Sesquiterpenes; D012987:Soil; D000094162:Phytoalexins