title: Total serum tryptase: a predictive marker for KIT mutation in acute myeloid leukemia.
abstract: Human tryptase is a serine protease expressed in mast-cells. We previously observed that AML blast cells, cultured in vitro from a KIT D816Y patient, give rise to adherent cells with mast-cell like phenotype and tryptase was released in the serum-free medium. To correlate total serum tryptase (ts-try) levels with cytogenetic features and KIT mutational status, we analyzed serum samples from AML patients at diagnosis. In 70 out of 155 patients (45%) we detected elevated ts-try (>15 ng/mL), significantly linked to t(8;21) (P < .001) and inv(16) (P = .007). In patients that achieved complete remission the ts-try decreased to normal values. In 75 patients screened for KIT mutation, we found a clear relationship between elevated ts-try and mutated patients with t(8;21) (P < .001). In conclusion, we propose that checking for ts-try at diagnosis of AML may be a simple tool to select patients to be addressed to KIT mutation screening.
authors.affiliation: Department of Oncology, Niguarda Hospital, Piazza Ospedale Maggiore, 3-20162 Milan, Italy. roberto.cairoli@ospedaleniguarda.it
authors.forename: Roberto
authors.initials: R
authors.lastname: Cairoli
authors.forename: Carla Barbara
authors.initials: CB
authors.lastname: Ripamonti
authors.forename: Alessandro
authors.initials: A
authors.lastname: Beghini
authors.forename: Simonetta
authors.initials: S
authors.lastname: Granata
authors.forename: Giovanni
authors.initials: G
authors.lastname: Grillo
authors.forename: Matteo
authors.initials: M
authors.lastname: Brioschi
authors.forename: Gianpaolo
authors.initials: G
authors.lastname: Nadali
authors.forename: Assunta
authors.initials: A
authors.lastname: Viola
authors.forename: Chiara
authors.initials: C
authors.lastname: Cattaneo
authors.forename: Liliana
authors.initials: L
authors.lastname: Inropido
authors.forename: Erika
authors.initials: E
authors.lastname: Ravelli
authors.forename: Giambattista
authors.initials: G
authors.lastname: Bertani
authors.forename: Laura
authors.initials: L
authors.lastname: Pezzetti
authors.forename: Michele
authors.initials: M
authors.lastname: Nichelatti
authors.forename: Alessandro
authors.initials: A
authors.lastname: Marocchi
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Rossi
authors.forename: Giovanni
authors.initials: G
authors.lastname: Pizzolo
authors.forename: Felicetto
authors.initials: F
authors.lastname: Ferrara
authors.forename: Anna Maria
authors.initials: AM
authors.lastname: Nosari
authors.forename: Enrica
authors.initials: E
authors.lastname: Morra
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000097:blood*; D016895:Culture Media, Serum-Free; D005260:Female; D006801:Humans; D015470:Leukemia, Myeloid, Acute / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D009154:Mutation; D019009:Proto-Oncogene Proteins c-kit / Q000235:genetics*; D055815:Young Adult
chemical_list: D014408:Biomarkers, Tumor; D016895:Culture Media, Serum-Free; D019009:Proto-Oncogene Proteins c-kit