Lactobacillus isolates from weaned piglets' mucosa with inhibitory activity against common porcine pathogens.
Twelve lactobacilli isolates from mucosa of 3-5-week-old weaned pigs were found to exert good antimicrobial activity against common porcine pathogens (S. aureus, B. cereus, E. coli, C. perfringens). Two of them produced in addition to lactic acid also considerable amounts of acetic acid, and 6 of them produced hydrogen peroxide and metabolites other than organic acids. Isolates 4/26 and 2/25 (identified as L. crispatus or L. amylovorus) were inhibitory against most strains of S. aureus, B. cereus and E. coli, and especially the strain 4/26 survived well in simulated gastric and intestinal juice. Diarrhea-causing E. coli O8K88H9 Ent(+) was successfully inhibited by the growing culture as well as by the catalase-treated and neutralized supernatant of L. reuteri 12/26. Mucin degradation and multiple resistance to antibiotics were not observed.
Hacin
B
B
Department of Animal Science, University of Ljubljana, Domzale, Slovenia.
Rogelj
I
I
Matijasić
B B
BB
D000818:Animals; D001409:Bacillus cereus / Q000187:drug effects; D003016:Clostridium perfringens / Q000187:drug effects; D003470:Culture Media / Q000737:chemistry / Q000494:pharmacology; D017077:Culture Media, Conditioned / Q000494:pharmacology; D004352:Drug Resistance, Microbial; D004926:Escherichia coli / Q000187:drug effects; D005243:Feces / Q000382:microbiology; D006861:Hydrogen Peroxide / Q000378:metabolism; D007082:Ileum / Q000382:microbiology*; D007113:Immunity, Innate; D007413:Intestinal Mucosa / Q000382:microbiology*; D019344:Lactic Acid / Q000494:pharmacology; D007778:Lactobacillus / Q000187:drug effects / Q000302:isolation & purification* / Q000378:metabolism; D009077:Mucins / Q000378:metabolism; D019936:Probiotics; D013211:Staphylococcus aureus / Q000187:drug effects; D034421:Sus scrofa / Q000382:microbiology*; D013553:Swine Diseases / Q000517:prevention & control; D014886:Weaning
D003470:Culture Media; D017077:Culture Media, Conditioned; D009077:Mucins; D019344:Lactic Acid; D006861:Hydrogen Peroxide