title: All-liquid separations, protein microarrays, and mass spectrometry to interrogate serum proteomes: an application to serum glycoproteomics.
abstract: Disease-related changes in serum proteins are reasonable targets for early detection particularly due to the noninvasive approach in obtaining samples. Glycoproteins specifically have been implicated in a variety of disease types ranging from immune diseases to cancers. High-throughput screening methods that can assess glycosylation states of all serum proteins in normal and diseased sample groups can facilitate early detection as well as shed light on disease progression mechanisms. Outlined here is a combination of liquid separation, protein microarray, and mass spectrometry approach to highlight candidate proteins involved in diseases through glycosylation mechanisms.
authors.affiliation: Department of Chemistry, University of Michigan, Ann Arbor, MI, USA.
authors.forename: Tasneem H
authors.initials: TH
authors.lastname: Patwa
authors.forename: Yinghua
authors.initials: Y
authors.lastname: Qiu
authors.forename: Jia
authors.initials: J
authors.lastname: Zhao
authors.forename: Diane M
authors.initials: DM
authors.lastname: Simeone
authors.forename: David M
authors.initials: DM
authors.lastname: Lubman
mesh_terms: D001798:Blood Proteins / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D006023:Glycoproteins / Q000097:blood*; D006031:Glycosylation; D006801:Humans; D037102:Lectins / Q000302:isolation & purification; D013058:Mass Spectrometry; D008567:Membranes, Artificial; D010449:Peptide Mapping; D016062:Porosity; D040081:Protein Array Analysis / Q000379:methods*; D020543:Proteome / Q000032:analysis*; D040901:Proteomics / Q000379:methods*; D014357:Trypsin / Q000378:metabolism
chemical_list: D001798:Blood Proteins; D006023:Glycoproteins; D037102:Lectins; D008567:Membranes, Artificial; D020543:Proteome; D014357:Trypsin