Role of nm23 in the regulation of cell shape and migration via Rho family GTPase signals.
Rho family small GTPase plays a key role in the regulation of cell shape and migration in mammalian cells. Constitutive activation of Rho GTPase leads to the aberrant cell morphology and migration. We identified nm23-H2 as a binding partner of Lbc proto-oncogene product, which specifically activates RhoA, and revealed that nm23-H2 could act as a negative regulator of Rho activity. Furthermore, we found that Lbc, nm23-H2 and ICAP1-alpha could form tertial complex in cells, and this complex formation was thought to be critical for cell migration stimulated by integrin. It is reported that nm23-H1 bound to Tiam1 and Dbl, which activates Rac and Cdc42 small GTPase, respectively. We discuss the role of nm23 in the regulation of cell morphology and cell migration via Rho family GTPases.
Miyamoto
Masaaki
M
Department of Biology, Graduate School of Science, Kobe University, 1-1 Rokkodai-cho, Nada, Kobe 657-8501, Japan. miya@kobe-u.ac.jp
Iwashita
Shinki
S
Yamaguchi
Satomi
S
Ono
Yoshitaka
Y
D054758:A Kinase Anchor Proteins / Q000235:genetics / Q000378:metabolism; D048868:Adaptor Proteins, Signal Transducing; D000595:Amino Acid Sequence; D000818:Animals; D019556:COS Cells; D002465:Cell Movement / Q000235:genetics*; D048430:Cell Shape / Q000235:genetics*; D002522:Chlorocebus aethiops; D004926:Escherichia coli / Q000235:genetics; D005982:Glutathione Transferase / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics / Q000378:metabolism; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism; D015778:Minor Histocompatibility Antigens; D008954:Models, Biological; D008969:Molecular Sequence Data; D054778:NM23 Nucleoside Diphosphate Kinases / Q000502:physiology*; D000090063:Proto-Oncogene Mas; D011518:Proto-Oncogene Proteins / Q000235:genetics / Q000378:metabolism; D011994:Recombinant Proteins / Q000378:metabolism; D015398:Signal Transduction / Q000235:genetics*; D014170:Transformation, Genetic; D020741:rho GTP-Binding Proteins / Q000235:genetics / Q000378:metabolism*
D054758:A Kinase Anchor Proteins; C085467:AKAP13 protein, human; D048868:Adaptor Proteins, Signal Transducing; C108257:ITGB1BP1 protein, human; D047908:Intracellular Signaling Peptides and Proteins; C000715470:MAS1 protein, human; D008565:Membrane Proteins; D015778:Minor Histocompatibility Antigens; D054778:NM23 Nucleoside Diphosphate Kinases; D000090063:Proto-Oncogene Mas; D011518:Proto-Oncogene Proteins; D011994:Recombinant Proteins; D005982:Glutathione Transferase; D020741:rho GTP-Binding Proteins