[The effect of focal-adhesion micromanipulation in trauma and wound healing].
OBJECTIVE
To explore the effect of focal-adhesion micromanipulation on the biological behavior of fibroblast.


METHODS
Micro-pot was made by microcontact printing. The molecules of constitutive protein was adhered on micro-pot by self-assemble of peptides. Skin fibroblasts were cultured on the membrane by self-made biomechanical cell culture for 2 weeks. Morphology observation and cell immunohistochemistry analysis was performed.


RESULTS
After 2 weeks, the morphology of the fibroblasts was diverse and more compliant. Cell immunohistochemistry analysis found that the expression of integrinbeta1, alpha5 and tensin was dramatically reduced.


CONCLUSIONS
The morphology and the biological behaviour of the fibroblasts in hypertrophic scar can be changed after micromanipulation of focal adhesion.
Jiang
Yu-zhi
YZ
Department of Burn, Burn Institute of Shanghai, Ruijin Hospital, Shanghai Jiaotong University, Shanghai, China.
Wei
Jun
J
Yuan
Bo
B
Liu
Ying-kai
YK
Wang
Zhi-yong
ZY
Ding
Kui-fu
KF
Wang
Xi-qiao
XQ
Tian
Ming
M
Li
Chun-sen
CS
Song
Fei
F
Qing
Chun
C
Lu
Shu-liang
SL
D018929:Cell Culture Techniques; D048708:Cell Growth Processes; D002478:Cells, Cultured; D002921:Cicatrix / Q000601:surgery*; D020405:Dermis / Q000166:cytology; D005260:Female; D005347:Fibroblasts / Q000166:cytology*; D022001:Focal Adhesions; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D014945:Wound Healing