[The application of three methods combined for extracting DNA from human epithelium].
OBJECTIVE
To improve DNA extraction from human epithelium.


METHODS
Chelex-100, Chelex-100 organic combined and Chelex-100 magnetism pearl combined methods were adopted according to the situation of each case.


RESULTS
With the stepwise extracting protocol, the trace amount of DNA was analyzed most efficiently and the success rate of DNA extracting was better improved.


CONCLUSIONS
The efficiency of DNA extraction from human epithelium could be greatly improved by modifying the extracting protocol such as purification and concentration based on previous results.
Guo
Yan-xia
YX
Forensic Science Institute of Public Security Ministry, Beijing 100038, China.
Chen
Song
S
Liu
Kai-hui
KH
D004247:DNA / Q000032:analysis / Q000302:isolation & purification*; D004847:Epithelial Cells / Q000166:cytology*; D053803:Forensic Genetics; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D012117:Resins, Synthetic / Q000737:chemistry
D012117:Resins, Synthetic; C024997:Chelex 100; D004247:DNA