[Effect of local treatment with cooling and spray film on early edema of superficial II degree scald burns in rats].
OBJECTIVE
To investigate the anti-inflammatory effect of early local treatment with cooling and spray film on scald burn injury in rats.


METHODS
Seventy-five Wistar rats were randomly divided into 5 groups including the sham-scalded group, untreated scald group, cooling group, spray film group, and cooling plus spray film group with corresponding treatments. After gross observation of the wounds, the tissues at the wounds were sampled at different time points after the injury to determine the total water content (wet: dry weight ratio) and prostaglandin E(2) (PGE(2)) levels using radioimmunoassay (RIA).


RESULTS
Treatment with cooling and spray film significantly alleviated the swelling and effusion of the scald burns. At each of the time points, the water content and PGE(2) levels in the cooling group, spray film group and cooling plus spray film group were all lower than those in untreated scald group (P<0.01), but all higher than those in the sham-scalded group (P<0.01). The water content and PGE(2) levels were the lowest in cooling plus spray film group, and a significant correlation was noted between the water content and PGE(2) levels in the untreated scald group, cooling group, spray film group and cooling plus spray film group (P<0.01).


CONCLUSIONS
Local treatment with cooling and spray film can alleviate the edema of superficial II degree scald burns in rats probably by reducing the levels of the inflammatory cytokines in the local tissues.
Huang
Hui-min
HM
Department of Burns, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China. huanghuimin123@tom.com
Wang
Jia-han
JH
Yang
Lei
L
Yi
Zhao-hui
ZH
D000818:Animals; D002056:Burns / Q000150:complications* / Q000378:metabolism / Q000473:pathology*; D017679:Cryotherapy; D015232:Dinoprostone / Q000378:metabolism; D004487:Edema / Q000150:complications* / Q000378:metabolism / Q000628:therapy*; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors
D015232:Dinoprostone