Functions of oligochitosan induced protein kinase in tobacco mosaic virus resistance and pathogenesis related proteins in tobacco.
Oligochitosan (OC) can regulate plant defense responses in many aspects, but the basic signal transduction pathway is still unclear. In this study, we used transgenic (TG) tobacco (Nicotiana Tabacum var. Samsun NN) as plant material whose oligochitosan induced protein kinase (OIPK) gene was inhibited by antisense transformation, to study the role of OIPK in tobacco defense reactions. The results showed that OIPK could increase tobacco resistance against tobacco mosaic virus (TMV), in that wild-type (WT) tobacco showed longer lesion appearance time, higher lesion inhibition ratio, smaller average final lesion diameter and lower average final lesion area percent to whole leaf area. It led us to analyze some pathogenesis related (PR) enzymes' activities and mRNA level, which played roles in tobacco resistance against TMV. We found that phenylalanine ammonia-lyase (PAL) and peroxidase (POD) activities were positively related to OIPK, but not polyphenol oxidase (PPO). It was also demonstrated that OIPK mRNA could be induced by OC, wound and TMV infection. In addition, OIPK could up-regulated three PR genes, PAL, chitinase (CHI) and beta-1, 3-glucanase (GLU) mRNA level to different extent. Taken together, these results implied that OIPK could function in tobacco resistance against both biotic and abiotic stress, possibly via various PR proteins.
Yafei
Chen
C
School of Sciences, Hebei University of Technology, Tianjin 300130, China.
Yong
Zhan
Z
Xiaoming
Zhao
Z
Peng
Guo
G
Hailong
An
A
Yuguang
Du
D
Yingrong
Han
H
Hui
Liu
L
Yuhong
Zhang
Z
D004156:Catechol Oxidase / Q000235:genetics / Q000378:metabolism; D002686:Chitin / Q000031:analogs & derivatives* / Q000378:metabolism; D002688:Chitinases / Q000235:genetics / Q000378:metabolism; D048271:Chitosan; D015870:Gene Expression; D018506:Gene Expression Regulation, Plant; D043326:Glucan 1,3-beta-Glucosidase / Q000235:genetics / Q000378:metabolism; D009844:Oligosaccharides; D009195:Peroxidase / Q000235:genetics / Q000378:metabolism; D010650:Phenylalanine Ammonia-Lyase / Q000235:genetics / Q000378:metabolism; D010935:Plant Diseases / Q000235:genetics / Q000821:virology*; D018515:Plant Leaves / Q000187:drug effects / Q000201:enzymology / Q000235:genetics; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D030821:Plants, Genetically Modified; D011494:Protein Kinases / Q000235:genetics* / Q000378:metabolism; D017346:Protein Serine-Threonine Kinases / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D015398:Signal Transduction; D014026:Nicotiana / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics; D014027:Tobacco Mosaic Virus / Q000472:pathogenicity*; D015854:Up-Regulation
D009844:Oligosaccharides; D010940:Plant Proteins; D012333:RNA, Messenger; C493484:oligochitosan; D002686:Chitin; D048271:Chitosan; D004156:Catechol Oxidase; D009195:Peroxidase; D011494:Protein Kinases; D017346:Protein Serine-Threonine Kinases; D002688:Chitinases; D043326:Glucan 1,3-beta-Glucosidase; D010650:Phenylalanine Ammonia-Lyase