title: [Inhibitory effects of RNA interference on MMP-24 expression and invasiveness of ovarian cancer SKOV(3) cells].
abstract: OBJECTIVE
  To investigate the inhibitory effect of RNA interference (RNAi) on MMP-24 expression and invasiveness of ovarian cancer SKOV(3) cells.
  
  
  METHODS
  Two pairs of small interfering RNA (siRNA) specific to MMP-24 mRNA were designed and transfected into SKOV(3) cells. RT-PCR and Western blotting were used to detect the mRNA and protein expressions of MMP-24, and the cell invasiveness was assessed using an in vitro invasion test.
  
  
  RESULTS
  After transfection with siRNA, the mRNA and protein expression levels of MMP-24 were obviously reduced in SKOV(3) cells, which also showed significantly decreased invasiveness in vitro.
  
  
  CONCLUSIONS
  MMP-24 gene silencing by RNAi can suppress the invasiveness of ovarian cancer SKOV(3) cells in vitro, which may provide a new therapeutic approach of ovarian cancer.
authors.affiliation: Department of Obstetrics and Gynecology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China. lyp76700@163.com
authors.forename: Ya-ping
authors.initials: YP
authors.lastname: Luo
authors.forename: Mei
authors.initials: M
authors.lastname: Zhong
authors.forename: Li-ping
authors.initials: LP
authors.lastname: Wang
authors.forename: Mei
authors.initials: M
authors.lastname: Zhong
authors.forename: Gui-qin
authors.initials: GQ
authors.lastname: Sun
authors.forename: Jing
authors.initials: J
authors.lastname: Li
mesh_terms: D015153:Blotting, Western; D045744:Cell Line, Tumor; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D053504:Matrix Metalloproteinases, Membrane-Associated / Q000172:deficiency* / Q000235:genetics* / Q000378:metabolism; D010051:Ovarian Neoplasms / Q000235:genetics / Q000473:pathology*; D010957:Plasmids / Q000235:genetics; D034622:RNA Interference; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D014162:Transfection
chemical_list: D012333:RNA, Messenger; C492243:MMP24 protein, human; D053504:Matrix Metalloproteinases, Membrane-Associated