Tempol reduces bacterial translocation after ischemia/reperfusion injury in a rat model of superior mesenteric artery occlusion.
OBJECTIVE
We investigated whether Tempol, a water-soluble antioxidant, prevents the harmful effects of superior mesenteric ischemia/reperfusion on intestinal tissues in rats.


METHODS
The rats were divided into three groups of 10. In group 1, the superior mesenteric artery (SMA) was isolated but not occluded, and in groups 2 and 3 the superior mesenteric artery was occluded for 60 min. After that, the clamp was removed and reperfusion began. In group 3, 5 min before the start of reperfusion, a bolus dose of 30 mg/kg Tempol was administered intravenously and continued at a dose of 30 mg/kg for 60 min. All animals were euthanized after 24 h and tissue samples were collected for analysis.


RESULTS
There was a significant increase in myeloperoxidase activity, malondialdehyde levels, and the incidence of bacterial translocation in group 2, with a decrease in glutathione levels. These parameters were found to be normalized in group 3. The intestinal mucosal injury score in group 2 was significantly higher than those in groups 1 and 3.


CONCLUSIONS
Tempol prevents bacterial translocation while precluding the harmful effects of ischemia/reperfusion injury on intestinal tissues in a rat model of superior mesenteric artery occlusion.
Berber
Ibrahim
I
Department of General Surgery, Haydarpasa Numune Training and Research Hospital, Istanbul, Turkey.
Aydin
Cagatay
C
Cevahir
Nural
N
Yenisey
Cigdem
C
Gumrukcu
Gulistan
G
Kocbil
Goksel
G
Tellioglu
Gurkan
G
Tekin
Koray
K
D000208:Acute Disease; D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage / Q000494:pharmacology*; D001157:Arterial Occlusive Diseases / Q000473:pathology*; D018988:Bacterial Translocation / Q000187:drug effects*; D003497:Cyclic N-Oxides / Q000008:administration & dosage / Q000494:pharmacology*; D007413:Intestinal Mucosa / Q000187:drug effects / Q000473:pathology*; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D008638:Mesenteric Arteries / Q000187:drug effects / Q000473:pathology*; D023421:Models, Animal; D017202:Myocardial Ischemia / Q000150:complications* / Q000517:prevention & control; D015425:Myocardial Reperfusion / Q000009:adverse effects*; D009504:Neutrophils / Q000187:drug effects / Q000201:enzymology; D051381:Rats; D017208:Rats, Wistar; D017382:Reactive Oxygen Species; D013113:Spin Labels
D000975:Antioxidants; D003497:Cyclic N-Oxides; D017382:Reactive Oxygen Species; D013113:Spin Labels; D008315:Malondialdehyde; C001803:tempol