title: Alveolar macrophages and CC chemokines are increased in children with cystic fibrosis.
abstract: Airway inflammation is an important component of cystic fibrosis (CF) lung disease. We sought to determine whether alveolar macrophages were involved in early CF lung disease. Children with CF (median age 3.1 yrs) participated in a surveillance programme that included annual bronchoalveolar lavage (BAL). Control samples were obtained from non-CF children (median age 3.1 yrs; n = 24) investigated for persistent respiratory symptoms. Pulmonary infection was detected in 31% (16 out of 51) and 38% (nine out of 24) of children from the CF and non-CF groups, respectively. Alveolar macrophages in BAL were increased in CF compared with non-CF in the absence of infection (223x10(3) versus 85x10(3) cells.mL(-1); p = 0.001) and were associated with elevations in the CC chemokines (macrophage inflammatory protein (MIP)-3alpha (chemokine (C-C motif) ligand (CCL)20; 355.8 versus 46.0 pg.mL(-1); p<0.001), monocyte chemotactic protein-1 (CCL2; 263.5 versus 25.3 pg.mL(-1); p<0.001), MIP-1alpha (CCL3; 38.2 versus 4.9 pg.mL(-1); p<0.001) and MIP-1beta (CCL4; 326.6 versus 27.5 pg.mL(-1); p<0.001)). Total cell counts and neutrophil numbers increased in the presence of infection; however, there was no additional effect of CF. Alveolar macrophages and CC chemokines are elevated in the lungs in young children with CF even in the absence of pulmonary infection. Longitudinal studies are required to determine the clinical relevance of these findings.
authors.affiliation: Telethon Institute for Child Health Research, West Perth, WA, Australia.
authors.forename: S
authors.initials: S
authors.lastname: Brennan
authors.forename: P D
authors.initials: PD
authors.lastname: Sly
authors.forename: C L
authors.initials: CL
authors.lastname: Gangell
authors.forename: N
authors.initials: N
authors.lastname: Sturges
authors.forename: K
authors.initials: K
authors.lastname: Winfield
authors.forename: M
authors.initials: M
authors.lastname: Wikstrom
authors.forename: S
authors.initials: S
authors.lastname: Gard
authors.forename: J W
authors.initials: JW
authors.lastname: Upham
mesh_terms: D018893:Bronchoalveolar Lavage; D016022:Case-Control Studies; D002452:Cell Count; D018932:Chemokine CCL2 / Q000378:metabolism*; D018946:Chemokine CCL5 / Q000378:metabolism*; D002675:Child, Preschool; D003550:Cystic Fibrosis / Q000378:metabolism* / Q000382:microbiology / Q000473:pathology*; D005260:Female; D006801:Humans; D007223:Infant; D008168:Lung / Q000378:metabolism / Q000473:pathology; D019402:Macrophage Inflammatory Proteins / Q000378:metabolism*; D016676:Macrophages, Alveolar / Q000502:physiology*; D008297:Male; D012141:Respiratory Tract Infections / Q000150:complications / Q000378:metabolism / Q000473:pathology
chemical_list: C111835:CCL2 protein, human; C483024:CCL5 protein, human; D018932:Chemokine CCL2; D018946:Chemokine CCL5; D019402:Macrophage Inflammatory Proteins