Impact of cytogenetic and genomic aberrations of the kallikrein locus in ovarian cancer.
The tissue kallikrein (KLK) genes are a new source for biomarkers in ovarian cancer. However, there has been no systematic analysis of copy number and structural rearrangements related to their protein expression. Chromosomal rearrangements and copy number changes of the KLK region were studied by FISH with protein levels measured by ELISA. Ovarian cancer and cell lines revealed the KLK region was subject to copy number imbalances or involved in unbalanced translocations and were associated with increased protein expression of KLKs 5, 6, 7, 8, 9, 10 and 11. In this initial study, we introduce the potential for long-range chromosomal effects and copy number as a mechanism for the previously reported aberrant expression of many KLK genes in ovarian cancers.
Bayani
Jane
J
Department of Applied Molecular Oncology, Ontario Cancer Institute, Princess Margaret Hospital, University Health Network, Toronto, Ontario, M5G 2M9, Canada.
Paliouras
Miltiadis
M
Planque
Chris
C
Shan
Shannon J C
SJ
Graham
Cassandra
C
Squire
Jeremy A
JA
Diamandis
Eleftherios P
EP
D002869:Chromosome Aberrations; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D018628:Gene Dosage; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007610:Kallikreins / Q000235:genetics*; D010051:Ovarian Neoplasms / Q000235:genetics*; D014178:Translocation, Genetic; D015854:Up-Regulation / Q000235:genetics
D007610:Kallikreins