Utility and evaluation of new variable-number tandem-repeat systems for genotyping mycobacterial tuberculosis isolates.
We compared mycobacterial interspersed repetitive unit (MIRU)-variable number tandem repeat (VNTR) typing to traditional spoligotyping for discriminating Mycobacterium tuberculosis (MTB) strains. Our 17-loci MIRU-VNTR typing method was found to be superior to spoligotyping for non-Beijing family strains. To extend the method we also established PCR-based rapid genotyping protocols for Beijing, East-African-Indian and U lineages.
Dou
Horng-Yunn
HY
Division of Clinical Research, National Health Research Institutes, Zhunan, Taiwan.
Lu
Jang-Jih
JJ
Lin
Chih-Wei
CW
Chang
Jia-Ru
JR
Sun
Jun-Ren
JR
Su
Ih-Jen
IJ
D015373:Bacterial Typing Techniques / Q000379:methods*; D016172:DNA Fingerprinting / Q000379:methods*; D005838:Genotype; D006801:Humans; D018598:Minisatellite Repeats; D009169:Mycobacterium tuberculosis / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000379:methods; D014397:Tuberculosis, Pulmonary / Q000382:microbiology*