[Effect of Shuanghuanglian injection on cerebral nuclear factor-kappaB expression in mice with viral encephalitis].
OBJECTIVE
To observe the effect of Shuanghuanglian injection on cerebral expression of nuclear factor-kappaB (NF-kappaB) in mice with viral encephalitis.


METHODS
The mice with experimental viral encephalitis received treatment with Shuanghuanglian injection at the dose of 0.2, 1.5, and 5 for 5, 10 or 20 consecutive days. The total RNA of the brain tissue was extracted to analyze the protein and mRNA expression of NF-kappaB using Western blotting and RT-PCR, respectively.


RESULTS
Compared with the control group, the mice with experimental viral encephalitis showed significantly increased protein and mRNA expressions of NF-kappaB (P<0.01). Treatment with Shuanghuanglian injection at the doses of 0.2 and 1.5 mg/kg significantly lowered NF-kappaB protein and mRNA expressions in the brain of mice with viral encephalitis (P<0.05), and the effect was even more obvious at the dose of 5 mg/kg (P<0.01).


CONCLUSIONS
Shuanghuanglian injection can reduce the expression of NF-kappaB in the brain of mice with viral encephalitis in a dose- and time-dependent manner.
Tian
Ye
Y
Department Neurology, Xi'an Central Hospital, Xi'an 710003 China.
DI
Zheng-li
ZL
Lei
Hui
H
Zhang
Ge-juan
GJ
Liang
Hua-di
HD
Chen
Hui-ling
HL
D000818:Animals; D015153:Blotting, Western; D001921:Brain / Q000187:drug effects* / Q000378:metabolism; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage* / Q000494:pharmacology* / Q000627:therapeutic use; D018792:Encephalitis, Viral / Q000188:drug therapy / Q000235:genetics / Q000378:metabolism*; D005786:Gene Expression Regulation / Q000187:drug effects*; D007267:Injections; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D016328:NF-kappa B / Q000235:genetics* / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction
D004365:Drugs, Chinese Herbal; D016328:NF-kappa B; D012333:RNA, Messenger; C516400:shuang-huang-lian