title: Detection of cancer stem cells from the C6 glioma cell line.
abstract: Various malignant cancers have been found to contain a sub-population of stem cell-like tumour cells, or cancer stem cells (CSCs), however, culture methods for CSCs and the size of the fraction of CSCs in C6, which is a commonly used glioma cell line, remain controversial. In this study, we demonstrated that the C6 cell line contains a fraction of tumour cells that can form tumour spheres in a simplified serum-free neural stem cell medium and express CD133 and nestin, which are widely-used markers for brain CSCs. Immunohistochemistry and immunofluorescence confirmed the existence of CSCs both in the C6 cell line and C6 xenografts. Flow cytometry demonstrated that 4.02% of cells in the C6 cell line and 4.21% in the C6 xenografts presented as CSCs. These results confirm the fraction of CSCs in the C6 cell line and provide a simple and effective method for isolation of CSCs to study the initiation and progression of human glioma and, possibly, other malignant tumours.
authors.affiliation: Department of Neurosurgery, Shandong University, Qilu Hospital, Jinan, Shandong, China.
authors.forename: X-D
authors.initials: XD
authors.lastname: Zhou
authors.forename: X-Y
authors.initials: XY
authors.lastname: Wang
authors.forename: F-J
authors.initials: FJ
authors.lastname: Qu
authors.forename: Y-H
authors.initials: YH
authors.lastname: Zhong
authors.forename: X-D
authors.initials: XD
authors.lastname: Lu
authors.forename: P
authors.initials: P
authors.lastname: Zhao
authors.forename: D-H
authors.initials: DH
authors.lastname: Wang
authors.forename: Q-B
authors.initials: QB
authors.lastname: Huang
authors.forename: L
authors.initials: L
authors.lastname: Zhang
authors.forename: X-G
authors.initials: XG
authors.lastname: Li
mesh_terms: D000071916:AC133 Antigen; D000818:Animals; D015703:Antigens, CD / Q000378:metabolism; D014408:Biomarkers, Tumor / Q000378:metabolism; D049109:Cell Proliferation; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D005910:Glioma / Q000473:pathology*; D006023:Glycoproteins / Q000378:metabolism; D007381:Intermediate Filament Proteins / Q000378:metabolism; D051379:Mice; D008819:Mice, Nude; D014411:Neoplastic Stem Cells / Q000473:pathology*; D009419:Nerve Tissue Proteins / Q000378:metabolism; D064231:Nestin; D010455:Peptides / Q000378:metabolism; D051381:Rats; D018874:Spheroids, Cellular / Q000473:pathology; D014407:Tumor Cells, Cultured; D023041:Xenograft Model Antitumor Assays
chemical_list: D000071916:AC133 Antigen; D015703:Antigens, CD; D014408:Biomarkers, Tumor; D006023:Glycoproteins; D007381:Intermediate Filament Proteins; C577484:NES protein, human; D009419:Nerve Tissue Proteins; C577485:Nes protein, mouse; C577486:Nes protein, rat; D064231:Nestin; C000606023:PROM1 protein, human; D010455:Peptides; C000606024:Prom1 protein, mouse; C000606025:Prom1 protein, rat