A xenon-129 biosensor for monitoring MHC-peptide interactions.
Caged in: The formation of a complex between a peptide ligand and a major histocompatibility complex (MHC) class II protein is detected by a (129)Xe biosensor. Cryptophane molecules that trap Xe atoms are modified with a hemagglutinin (HA) peptide, which binds to the MHC protein. The interaction can be monitored by an NMR chemical shift change of cage-HA bound (129)Xe.
Schlundt
Andreas
A
Protein Engineering Group, Leibniz-Institut für Molekulare Pharmakologie und FU Berlin, Robert-Roessle-Strasse 10, 13125 Berlin, Germany.
Kilian
Wolfgang
W
Beyermann
Michael
M
Sticht
Jana
J
Günther
Sebastian
S
Höpner
Sabine
S
Falk
Kirsten
K
Roetzschke
Olaf
O
Mitschang
Lorenz
L
Freund
Christian
C
D015374:Biosensing Techniques; D002460:Cell Line; D006388:Hemagglutinins / Q000737:chemistry; D000949:Histocompatibility Antigens Class II / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D046911:Macromolecular Substances / Q000737:chemistry; D019906:Nuclear Magnetic Resonance, Biomolecular; D011083:Polycyclic Compounds; D025941:Protein Interaction Mapping / Q000379:methods*; D014230:Triazoles / Q000737:chemistry; D014979:Xenon Isotopes / Q000737:chemistry*
D006388:Hemagglutinins; D000949:Histocompatibility Antigens Class II; D046911:Macromolecular Substances; D011083:Polycyclic Compounds; D014230:Triazoles; D014979:Xenon Isotopes; C523242:cryptophane