title: Up-regulation of L type amino acid transporter 1 after spinal cord injury in rats.
abstract: BACKGROUND
  L-type amino acid transporter 1 (LAT1) is proposed to be a major nutrient transporter at the blood brain barrier. LAT1 requires the heavy chain of 4F2 cell surface antigen (4F2hc) for functional expression.
  
  
  METHODS
  We investigated the expression of this heterodimeric transporter after traumatic spinal cord injury in rat by using immunohistochemical and western blot analyses.
  
  
  RESULTS
  LAT1 immunoreactivities were up-regulated in the capillary endothelia in close to the injury epicenter 24 hours after injury. It reached a peak at 48 hours after injury, and thereafter decreased. 4F2hc was abundant and unchanged all through the time course after SCI. Western blot analysis under reductive and non-reductive conditions showed that LAT1 and 4F2hc were conjugated as a heterodimeric transporter and the functional regulation was dependent on the light chain, LAT1.
  
  
  CONCLUSIONS
  We suggest that LAT1 may be transiently upregulated as part of the tissue-repair process after traumatic contusion injury in the spinal cord.
authors.affiliation: Department of Neurosurgery, National Defense Medical College, 3-2 Namiki, Tokorozawa, Saitama 359-8513, Japan. teru-toy@mse.biglobe.ne.jp
authors.forename: Terushige
authors.initials: T
authors.lastname: Toyooka
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Nawashiro
authors.forename: Nariyoshi
authors.initials: N
authors.lastname: Shinomiya
authors.forename: Akiko
authors.initials: A
authors.lastname: Yano
authors.forename: Hidetoshi
authors.initials: H
authors.lastname: Ooigawa
authors.forename: Atsushi
authors.initials: A
authors.lastname: Ohsumi
authors.forename: Yoichi
authors.initials: Y
authors.lastname: Uozumi
authors.forename: Youichi
authors.initials: Y
authors.lastname: Yanagawa
authors.forename: Hirotaka
authors.initials: H
authors.lastname: Matsuo
authors.forename: Katsuji
authors.initials: K
authors.lastname: Shima
mesh_terms: D027062:Amino Acid Transport System L / Q000378:metabolism*; D000704:Analysis of Variance; D000818:Animals; D004195:Disease Models, Animal; D005260:Female; D027282:Fusion Regulatory Protein 1, Heavy Chain / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D013116:Spinal Cord / Q000378:metabolism; D013119:Spinal Cord Injuries / Q000378:metabolism* / Q000473:pathology / Q000503:physiopathology; D013997:Time Factors; D015854:Up-Regulation / Q000502:physiology*
chemical_list: D027062:Amino Acid Transport System L; D027282:Fusion Regulatory Protein 1, Heavy Chain