title: Crystallization and preliminary X-ray crystallographic analysis of a novel histidinol-phosphate phosphatase from Thermococcus onnurineus NA1.
abstract: The TON_0887 gene product from Thermococcus onnurineus NA1 is a 240-residue protein that has histidinol-phosphate phosphatase (HolPase) activity. According to analysis of its primary structure, the TON_0887 gene product is a monofunctional HolPase that belongs to the DDDD superfamily. This contrasts with the generally accepted classification that bifunctional HolPases belong to the DDDD superfamily. The TON_0887 gene product was purified and crystallized at 295 K. A 2.2 A resolution data set was collected using synchrotron radiation. The TON-HolPase crystals belonged to space group P222(1), with unit-cell parameters a = 40.88, b = 46.89, c = 148.03 A. Assuming the presence of one molecule in the asymmetric unit, the solvent content was estimated to be about 48.3%.
authors.affiliation: Marine and Extreme Genome Research Center, Korea Ocean Research and Development Institute, Ansan, Republic of Korea.
authors.forename: Ha Il
authors.initials: HI
authors.lastname: Jung
authors.forename: Hyun Sook
authors.initials: HS
authors.lastname: Lee
authors.forename: Young Jun
authors.initials: YJ
authors.lastname: An
authors.forename: Yona
authors.initials: Y
authors.lastname: Cho
authors.forename: Jung Hyun
authors.initials: JH
authors.lastname: Lee
authors.forename: Sung Gyun
authors.initials: SG
authors.lastname: Kang
authors.forename: Sun Shin
authors.initials: SS
authors.lastname: Cha
mesh_terms: D003460:Crystallization; D018360:Crystallography, X-Ray; D006642:Histidinol-Phosphatase / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D019712:Thermococcus / Q000201:enzymology* / Q000235:genetics
chemical_list: D006642:Histidinol-Phosphatase