Synthesis and characterization of reactive poloxamer 407s for biomedical applications.
The drawbacks of poloxamer hydrogel, such as dissolving quickly in aqueous solution, have limited its biomedical application. In order to improve the stability of hydrogel, a novel system was developed by combining the reversible thermo-sensitive property of poloxamer 407 and the thiol-ene reactivity between the acrylate and thiol groups. It was found that the sol-gel transition of the acrylate/thiol modified poloxamer 407 mixture could be achieved at body temperature even with a low concentration of 17.5 wt.%. Meanwhile, the reaction between the acrylate and thiol modified poloxamer 407s occurred spontaneously in mimic physiological conditions, thus the hydrogel with crosslinking structure was formed. As a result, the stability of the crosslinked hydrogel was enhanced remarkably, and the release time of the drug from the crosslinked hydrogel was about 4.0 times as long as that from the poloxamer 407 hydrogel. Invitro and invivo experiments revealed that the biocompatibilities of the modified poloxamer 407 hydrogel were similar to that of poloxamer 407. These results indicate that the modified poloxamer 407s have potential applications in controlled drug release, tissue engineering and cell encapsulation etc.
Niu
Guoguang
G
School of Materials Science and Engineering, University of Science and Technology, Beijing, China.
Du
Fengyi
F
Song
Li
L
Zhang
Hongbin
H
Yang
Jun
J
Cao
Hui
H
Zheng
Yudong
Y
Yang
Zhou
Z
Wang
Guojie
G
Yang
Huai
H
Zhu
Siquan
S
D000818:Animals; D016466:CHO Cells; D049109:Cell Proliferation / Q000187:drug effects; D006224:Cricetinae; D003412:Cricetulus; D003692:Delayed-Action Preparations / Q000008:administration & dosage / Q000138:chemical synthesis / Q000737:chemistry; D005260:Female; D020100:Hydrogels / Q000008:administration & dosage / Q000138:chemical synthesis / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D007273:Injections, Intramuscular; D008958:Models, Molecular; D044367:Phase Transition; D020442:Poloxamer / Q000008:administration & dosage / Q000138:chemical synthesis / Q000737:chemistry*; D051381:Rats; D017208:Rats, Wistar; D012212:Rheology; D013438:Sulfhydryl Compounds / Q000737:chemistry; D013816:Thermodynamics
D003692:Delayed-Action Preparations; D020100:Hydrogels; D013438:Sulfhydryl Compounds; D020442:Poloxamer