[Effect of the drug-resistant attenuated Bacillus proteus on CD80 and CD86 protein expression in peripheral blood dendritic cells of hepatitis B patients].
OBJECTIVE
To explore the effect of the drug-resistant attenuated Bacillus proteus on the protein expression of CD80 and CD86 in peripheral blood dendritic cells (DCs) of hepatitis B patients.


METHODS
Peripheral blood monocytes were isolated from HBV-infected patients and the DCs were separated and induced to differentiate in vitro. The expression of CD80 and CD86 proteins on cultured DCs were examined using flow cytometry.


RESULTS
The expression rate of CD80 and CD86 of chronic hepatitis B patients increased significantly (P=0.000), while the positive expression rate of CD80 and CD86 showed no obvious variation in healthy individuals (P=0.185 and P=0.118, respectively).


CONCLUSIONS
Bacillus proteus can significantly increase CD80- and CD86-positive cell percentage in peripheral blood DCs of HBV-infected patients, but has no such an effect in healthy individuals.
Wang
Peng
P
Jiangdu Hospital, Southern Medical University, Guangzhou 510450, China. wangpeng72 wp@163.com
Shu
Si-yun
SY
Hu
Yan-qun
YQ
D000293:Adolescent; D000328:Adult; D018122:B7-1 Antigen / Q000378:metabolism*; D051940:B7-2 Antigen / Q000378:metabolism*; D001407:Bacillus / Q000187:drug effects / Q000276:immunology*; D016022:Case-Control Studies; D002648:Child; D003713:Dendritic Cells / Q000276:immunology / Q000378:metabolism*; D024881:Drug Resistance, Bacterial; D005260:Female; D005786:Gene Expression Regulation / Q000276:immunology*; D006509:Hepatitis B / Q000276:immunology* / Q000378:metabolism; D006801:Humans; D008297:Male; D008875:Middle Aged; D014613:Vaccines, Attenuated / Q000276:immunology; D055815:Young Adult
D018122:B7-1 Antigen; D051940:B7-2 Antigen; D014613:Vaccines, Attenuated