title: Protective effect of total aralosides of Aralia elata (Miq) Seem (TASAES) against diabetic cardiomyopathy in rats during the early stage, and possible mechanisms.
abstract: Total aralosides of Aralia elata (Miq) Seem (TASAES) from Chinese traditional herb Longya Aralia chinensis L was found to improve cardiac function. The present study was to determine the protective effects of TASAES on diabetic cardiomyopathy, and the possible mechanisms. Therefore, a single dose of streptozotocin was used to induce diabetes in Wister rats. Diabetic rats were immediately treated with low, medium and high doses of TASAES at 4.9, 9.8 mg/kg and 19.6 mg/kg body weight by gavage, respectively, for eight weeks. Cardiac function was evaluated by in situ hemodynamic measurements, and patch clamp for the L-type Ca2+ channel current I(Ca(2+)-L) and transient outward K+ channel current (I(to)). Histopathological changes were observed under light and electron microscope. The expression of pro-fibrotic factor, connective tissue growth factor (CTGF) was monitored using immunohistochemistry staining. Compared with diabetic group, medium and high doses, but not low dose, of TASAES showed a significant protection against diabetes-induced cardiac dysfunction, shown by increased absolute value of left ventricular systolic pressure (LVSP) and maximum rates of pressure development (+/-dp/dt(max)), and enhanced amplitude of I(Ca(2+)-L) (P<0.05). Histological staining indicated a significant inhibition of diabetes-caused pathological changes and up-regulation of CTGF expression (P< 0.05). The results suggest that TASAES prevents diabetes-induced cardiac dysfunction and pathological damage through up-regulating I(Ca(2+)-L) in cardiac cells and decreasing CTGF expression.
authors.affiliation: Department of Endocrinology, at the First Hospital, Changchun 130021, China.
authors.forename: Shugang
authors.initials: S
authors.lastname: Xi
authors.forename: Guihua
authors.initials: G
authors.lastname: Zhou
authors.forename: Xuexin
authors.initials: X
authors.lastname: Zhang
authors.forename: Wenjie
authors.initials: W
authors.lastname: Zhang
authors.forename: Lu
authors.initials: L
authors.lastname: Cai
authors.forename: Chunyan
authors.initials: C
authors.lastname: Zhao
mesh_terms: D000818:Animals; D027983:Aralia / Q000737:chemistry*; D020746:Calcium Channels, L-Type / Q000502:physiology; D009202:Cardiomyopathies / Q000188:drug therapy* / Q000209:etiology / Q000503:physiopathology; D055513:Connective Tissue Growth Factor / Q000378:metabolism; D003921:Diabetes Mellitus, Experimental / Q000150:complications*; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D006321:Heart / Q000187:drug effects / Q000503:physiopathology; D006439:Hemodynamics; D008297:Male; D009206:Myocardium / Q000473:pathology; D009828:Oleanolic Acid / Q000031:analogs & derivatives / Q000627:therapeutic use; D018408:Patch-Clamp Techniques; D015221:Potassium Channels / Q000502:physiology; D051381:Rats; D017208:Rats, Wistar; D012503:Saponins / Q000627:therapeutic use*; D016896:Treatment Outcome
chemical_list: D020746:Calcium Channels, L-Type; D004365:Drugs, Chinese Herbal; D015221:Potassium Channels; D012503:Saponins; D055513:Connective Tissue Growth Factor; D009828:Oleanolic Acid