Quantitative analysis of nargenicin in Nocardia sp. CS682 culture by high performance liquid chromatography.
A simple and sensitive HPLC method was developed for the quantitative analysis of nargenicin in the culture broth of Nocardia sp. CS682, isolated from Korean soil. Nargenicin, an unusual macrolide antibiotic with potent anti-MRSA (methicilin-resistant Staphylococcus aureus) activity, was extracted from the culture broth with ethylacetate and then analyzed by HPLC using solvent A (0.5 M dipotassium phosphate pH 2.5-water: acetonitrile = 80:20, v/v) and solvent B (100% acetonitrile), at 215 nm. The calibration curve of nargenicin was linear with correlation coefficient greater than 0.99. This method provides precise analysis of the nargenicin amount present in the cell free culture medium, and may be applicable to analyze other bacterial cultures containing similar compounds.
Cho
Seung Sik
SS
Department of Pharmacy, Chosun University, Gwang Ju, 501-759, Korea.
Sohng
Jae Kyung
JK
Lee
Hyo Jeong
HJ
Park
Sung Ju
SJ
Simkhada
Jaya Ram
JR
Yoo
Jin Cheol
JC
D000085:Acetates / Q000737:chemistry; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism / Q000494:pharmacology; D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000592:standards; D003470:Culture Media / Q000378:metabolism; D007723:Korea; D007783:Lactones / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D018942:Macrolides / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism / Q000494:pharmacology; D055624:Methicillin-Resistant Staphylococcus aureus / Q000187:drug effects / Q000254:growth & development; D015394:Molecular Structure; D009615:Nocardia / Q000378:metabolism*; D015203:Reproducibility of Results; D012988:Soil Microbiology; D012997:Solvents / Q000737:chemistry; D013997:Time Factors
D000085:Acetates; D000900:Anti-Bacterial Agents; D003470:Culture Media; D007783:Lactones; D018942:Macrolides; D012997:Solvents; C034624:nargenicin A1; C007650:ethyl acetate