A novel short hairpin RNA (shRNA) expression system promotes Sox9-dependent gene silencing.
Cartilage development and function are dependent on a temporally integrated program of gene expression. With the advent of RNA interference (RNAi), artificial control of these complex programs becomes a possibility, limited only by the ability to regulate and express the RNAi. Using existing methods for production of RNAi's, we have constructed a plasmid-based short hairpin RNA (shRNA) expression system under control of the human pol III H1 promoter and supplemented this promoter with DNA binding sites for the cartilage-specific transcription factor Sox9. The resulting shRNA expression system displays robust, Sox9-dependent gene silencing. Dependence on Sox9 expression was confirmed by electrophoretic mobility shift assays. The ability of the system to regulate heterologously expressed Sox9 was demonstrated by Western blot, as a function of both Sox9 to shRNA ratio, as well as time from transfection. This novel expression system supports auto-regulatory gene silencing, providing a tissue-specific feedback mechanism for temporal control of gene expression. Its applications for both basic mechanistic studies and therapeutic purposes should facilitate the design and implementation of innovative tissue engineering strategies.
Gilbert
James R
JR
Department of Orthopaedic Surgery, Thomas Jefferson University, Philadelphia, PA 19107, USA. jrg111@jefferson.edu
Adams
Christopher S
CS
Shapiro
Irving M
IM
Hickok
Noreen J
NJ
D002460:Cell Line; D005786:Gene Expression Regulation; D020868:Gene Silencing; D005821:Genetic Techniques; D049452:Green Fluorescent Proteins / Q000378:metabolism; D006801:Humans; D009690:Nucleic Acid Conformation; D010957:Plasmids / Q000235:genetics; D011401:Promoter Regions, Genetic / Q000235:genetics; D034741:RNA, Small Interfering / Q000737:chemistry / Q000235:genetics*; D055758:SOX9 Transcription Factor / Q000378:metabolism*; D013997:Time Factors
D034741:RNA, Small Interfering; D055758:SOX9 Transcription Factor; D049452:Green Fluorescent Proteins