title: Pericellular matrix of decidua-derived mesenchymal cells: a potent human-derived substrate for the maintenance culture of human ES cells.
abstract: In routine culture, human embryonic stem (hES) cells are maintained on either feeder cells or special culture substrates such as Matrigel. However, to expand hES cells for clinical applications, it is desirable to minimize animal-derived materials in the culture for safety reasons. In this report, we show that the pericellular matrix prepared from human decidua-derived mesenchymal cells (PCM-DM) is a potent substrate material that supports the growth and pluripotency of hES cells as efficiently as Matrigel does. This supporting activity of PCM-DM is stable and can be preserved for several months in the refrigerator. PCM-DM-based culture is compatible with non-conditioned commercial defined medium, and with the maintenance of dissociated hES cells in the presence of ROCK inhibitor. Since decidual mesenchymal cells can be prepared and expanded in a large quantity, PCM-DM is a practical human-derived substitute for the animal-derived substrates for use in clinical-grade culture of hES cells.
authors.affiliation: Organogenesis and Neurogenesis Group, Center for Developmental Biology, RIKEN, Kobe, Japan.
authors.forename: Tomoko
authors.initials: T
authors.lastname: Nagase
authors.forename: Morio
authors.initials: M
authors.lastname: Ueno
authors.forename: Michiru
authors.initials: M
authors.lastname: Matsumura
authors.forename: Keiko
authors.initials: K
authors.lastname: Muguruma
authors.forename: Masatoshi
authors.initials: M
authors.lastname: Ohgushi
authors.forename: Naomi
authors.initials: N
authors.lastname: Kondo
authors.forename: Daisuke
authors.initials: D
authors.lastname: Kanematsu
authors.forename: Yonehiro
authors.initials: Y
authors.lastname: Kanemura
authors.forename: Yoshiki
authors.initials: Y
authors.lastname: Sasai
mesh_terms: D000577:Amides / Q000494:pharmacology; D018929:Cell Culture Techniques; D003656:Decidua / Q000737:chemistry / Q000166:cytology*; D053595:Embryonic Stem Cells / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D005109:Extracellular Matrix / Q000737:chemistry; D005260:Female; D006801:Humans; D008648:Mesoderm / Q000737:chemistry / Q000166:cytology; D011725:Pyridines / Q000494:pharmacology
chemical_list: D000577:Amides; D011725:Pyridines; C108830:Y 27632