title: Effect of silicon dioxide on expression of poly (ADP-ribose) polymerase mRNA and protein.
abstract: Silicon dioxide induces acute injury and chronic pulmonary fibrosis. International Agency for Research on Cancer (IARC) listed it as a human carcinogen in 1996. However, the molecular mechanisms to induce cancer are not understood yet. The content of poly (ADP-ribose) polymerases (PARP) mRNA and protein in Hela cells treated with concentrations of silicon dioxide up to 400microg/ml was determined by real-time fluorogenetic quantitative PCR (RQ-PCR) and immunofluorescence assay, respectively. MTT assay was used to determine cell viability. The results showed that viability at 400microg/ml silica was significantly decreased but not at lower concentrations. The protein content of gamma-H2AX in silica-treated group was significantly higher than the controls. The PARP mRNA and protein levels were significantly reduced with a dose response manner from the lowest silicon dioxide level. Our findings suggested that silicon dioxide increased the expression of gamma-H2AX and inhibited the expression of PARP mRNA and protein in Hela cells.
authors.affiliation: School of Public Health and Family Medicine, Capital Medical University, Beijing, China.
authors.forename: Ai
authors.initials: A
authors.lastname: Gao
authors.forename: Shanshan
authors.initials: S
authors.lastname: Song
authors.forename: Danlin
authors.initials: D
authors.lastname: Wang
authors.forename: Wei
authors.initials: W
authors.lastname: Peng
authors.forename: Lin
authors.initials: L
authors.lastname: Tian
mesh_terms: D045744:Cell Line, Tumor; D006367:HeLa Cells; D006657:Histones / Q000378:metabolism; D006801:Humans; D011065:Poly(ADP-ribose) Polymerases / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D012822:Silicon Dioxide / Q000494:pharmacology* / Q000633:toxicity
chemical_list: C468783:H2AX protein, human; D006657:Histones; D012333:RNA, Messenger; D012822:Silicon Dioxide; D011065:Poly(ADP-ribose) Polymerases