Simultaneous determination of voriconazole and posaconazole concentrations in human plasma by high-performance liquid chromatography.
A simple, sensitive, and selective high-performance liquid chromatographic method for the simultaneous determination of voriconazole and posaconazole concentrations in human plasma was developed and validated. Quantitative recovery following liquid-liquid extraction with diethyl ether was achieved. Linearity ranged from 0.10 to 20.0 microg/ml for voriconazole and from 0.05 to 10.0 microg/ml for posaconazole. The intra- and interday coefficients of variation were less than 8.5%, and the lower limits of quantitation were <0.05 microg/ml.
Kahle
Kathrin
K
Department of Internal Medicine II, Division of Infectious Diseases, University of Wuerzburg Medical Center, Josef-Schneider Strasse 2, Wuerzburg, Germany.
Langmann
Peter
P
Schirmer
Diana
D
Lenker
Ulrike
U
Keller
Daniela
D
Helle
Annegret
A
Klinker
Hartwig
H
Heinz
Werner J
WJ
D000935:Antifungal Agents / Q000097:blood*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D011743:Pyrimidines / Q000097:blood*; D015203:Reproducibility of Results; D014230:Triazoles / Q000097:blood*; D065819:Voriconazole
D000935:Antifungal Agents; D011743:Pyrimidines; D014230:Triazoles; C101425:posaconazole; D065819:Voriconazole