title: How to predict implantation? No correlation between embryonic aneuploidy and soluble human leukocyte antigen G-concentrations.
abstract: OBJECTIVE
  To determine if soluble human leukocyte antigen-G (sHLA-G) concentrations in spent culture media may assist in identifying the normal embryo for implantation.
  
  
  METHODS
  Prospective blinded comparative study.
  
  
  METHODS
  Reproductive genetic and reproductive medicine centers.
  
  
  METHODS
  One hundred and sixteen embryos obtained from eight patients undergoing in vitro fertilization (IVF) with preimplantation genetic diagnosis (PGD).
  
  
  METHODS
  Culture media obtained 2 days after fertilization were analyzed for sHLA-G concentrations using an enzyme-linked immunosorbent assay (ELISA) assay. A sHLA-G concentration of >or=1.9 mIU/mL was considered a positive predictor for successful implantation. Polar bodies and blastomeres from day-3 embryos were tested by PGD for 5 to 11 chromosomes: 8, 9, 13, 15, 16, 17, 18, 21, 22, X, and Y.
  
  
  METHODS
  The results of the sHLA-G concentrations were compared with the results of the PGD analyses.
  
  
  RESULTS
  We found an sHLA-G concentration >or=1.9 mIU/mL in 48% (56 out of 116) and normal PGD results in 52% (57 out of 116) of embryos. Of the embryos with normal PGD results, 46% (26 out of 57) had sHLA-G concentrations >or=1.9 mIU/mL. Among the embryos with sHLA-G >or=1.9 mIU/mL, 46% (26 out of 56) had normal PGD results, and 21% of embryos displayed both normal PGD results and sHLA-G >or=1.9 mIU/mL.
  
  
  CONCLUSIONS
  No correlation between concentrations of sHLA-G in embryo culture media and PGD results of an embryo's aneuploidy were observed.
authors.affiliation: Rinehart Center for Reproductive Medicine, Evanston, Illinois, USA. cbcoulam@aol.com
authors.forename: Carolyn B
authors.initials: CB
authors.lastname: Coulam
authors.forename: Roumen G
authors.initials: RG
authors.lastname: Roussev
authors.forename: Sveltana
authors.initials: S
authors.lastname: Lerner
authors.forename: Zev
authors.initials: Z
authors.lastname: Zlatopolsky
authors.forename: Yuri
authors.initials: Y
authors.lastname: Ilkevitch
authors.forename: Ilan
authors.initials: I
authors.lastname: Tur-Kaspa
mesh_terms: D000782:Aneuploidy; D001757:Blastomeres / Q000502:physiology; D002869:Chromosome Aberrations / Q000706:statistics & numerical data; D002877:Chromosomes, Human / Q000235:genetics; D041321:Chromosomes, Human, X / Q000235:genetics; D041322:Chromosomes, Human, Y / Q000235:genetics; D047108:Embryonic Development / Q000502:physiology*; D005260:Female; D005307:Fertilization in Vitro; D006680:HLA Antigens / Q000097:blood*; D059951:HLA-G Antigens; D015395:Histocompatibility Antigens Class I / Q000097:blood*; D006801:Humans; D011237:Predictive Value of Tests; D011247:Pregnancy; D011256:Pregnancy Outcome; D019836:Preimplantation Diagnosis / Q000379:methods*
chemical_list: D006680:HLA Antigens; D059951:HLA-G Antigens; D015395:Histocompatibility Antigens Class I