Markers of Chlamydia pneumoniae and human cytomegalovirus infection in patients with chronic peripheral vascular disease and their relation to inflammation, endothelial dysfunction and changes in lipid metabolism.
Our aim was to detect markers of Chlamydia pneumoniae (CPN) and human cytomegalovirus (HCMV) infection in patients with peripheral vascular occlusive disease and to follow markers of inflammation, endothelial dysfunction and lipid metabolism alteration in patients with active infection. CPN genome was detected in 9 (47.4 %) patients by at least one PCR method. Serological markers of acute CPN infection were found in 5 (26.3 %) subjects; each of them showed also positivity in at least one of the PCR methods. HCMV DNA were detected in 2 (10.5 %) patients; HCMV-specific antibodies were detected in 14 (73.7 %) subjects, however only in IgG subclass. Subjects with HCMV PCR positivity thus showed no serological markers of active HCMV infection. Laboratory findings of acute CPN infection were associated with increased plasma levels of Lp(a), triacylglycerols, atherogenic index of plasma and E-selectin (p < 0.05). No significant differences were found in the other markers, including plasma levels of total cholesterol, ferritin, homocysteine, oxidized LDL, IL-6, IL-8, IL-18, TNF-alpha, soluble forms of VCAM-1 and ICAM-1, von Willebrand factor, C-reactive protein, and plasma nitrites & nitrates. Frequent presence of chlamydial DNA in atheromatous plaques from patients with peripheral vascular disease was confirmed. HCMV DNA was detected only sporadically and with positivity in anamnestic anti-HCMV antibodies (IgG) only, indicating a rare presence of latent virus rather than active replication. Patients with laboratory markers of acute CPN infection exhibited more pronounced alterations in lipid metabolism and endothelial dysfunction.
Kraml
P J
PJ
2nd Department of Internal Medicine, Charles University in Prague and Královské Vinohrady University Hospital, Czechia. kraml@fnkv.cz
Roubalová
K
K
Bulvas
M
M
Sommerová
Z
Z
PotoCková
J
J
Mandys
V
V
Andel
M
M
D000328:Adult; D000368:Aged; D050197:Atherosclerosis / Q000000981:diagnostic imaging / Q000209:etiology* / Q000378:metabolism / Q000382:microbiology / Q000503:physiopathology / Q000821:virology; D015415:Biomarkers; D023521:Chlamydophila Infections / Q000150:complications* / Q000378:metabolism / Q000382:microbiology / Q000503:physiopathology; D016993:Chlamydophila pneumoniae / Q000302:isolation & purification*; D003251:Constriction, Pathologic; D016207:Cytokines / Q000097:blood; D003586:Cytomegalovirus Infections / Q000150:complications* / Q000378:metabolism / Q000503:physiopathology / Q000821:virology; D004269:DNA, Bacterial / Q000032:analysis / Q000097:blood; D004279:DNA, Viral / Q000032:analysis / Q000097:blood; D050171:Dyslipidemias / Q000209:etiology*; D004730:Endothelium, Vascular / Q000503:physiopathology*; D005260:Female; D005263:Femoral Artery / Q000000981:diagnostic imaging / Q000378:metabolism / Q000382:microbiology / Q000473:pathology* / Q000821:virology; D006801:Humans; D007511:Ischemia / Q000209:etiology; D007866:Leg / Q000098:blood supply; D017270:Lipoprotein(a) / Q000097:blood; D008297:Male; D008875:Middle Aged; D016491:Peripheral Vascular Diseases / Q000000981:diagnostic imaging / Q000209:etiology* / Q000378:metabolism / Q000382:microbiology / Q000503:physiopathology / Q000821:virology; D011150:Popliteal Artery / Q000000981:diagnostic imaging / Q000378:metabolism / Q000382:microbiology / Q000473:pathology* / Q000821:virology; D011859:Radiography; D014657:Vasculitis / Q000209:etiology* / Q000378:metabolism / Q000382:microbiology / Q000503:physiopathology / Q000821:virology; D055815:Young Adult
D015415:Biomarkers; D016207:Cytokines; D004269:DNA, Bacterial; D004279:DNA, Viral; D017270:Lipoprotein(a)