Sol-gel derived nano-structured zinc oxide film for sexually transmitted disease sensor.
A 20-mer thiolated oligonucleotide probe (th-ssDNA) specific to Neisseria gonorrhoeae immobilized onto a sol-gel derived nano-structured zinc oxide (ZnO) film dip-coated onto an indium-tin-oxide (ITO) glass substrate has been used for the fabrication of a DNA biosensor for sexually transmitted disease (gonorrhoea) detection using hybridization technique. The results of characterization studies carried out on this th-ssDNA-ZnO/ITO bioelectrode using X-ray diffraction, UV-Visible, Fourier-transform infrared, scanning electron microscopy and electrochemical techniques reveal the linearity as 0.000524 fmol-0.524 nmol, with a detection limit of 0.000704 fmol within 60 s.
Ansari
Anees A
AA
Department of Science & Technology Centre on Biomolecular Electronics, National Physical Laboratory, Dr. K S Krishnan Marg, New Delhi 110012, India. aneesaansari@gmail.com
Singh
Renu
R
Sumana
G
G
Malhotra
B D
BD
D055664:Electrochemical Techniques / Q000295:instrumentation / Q000379:methods; D004566:Electrodes; D006069:Gonorrhea / Q000175:diagnosis*; D006801:Humans; D049329:Nanostructures / Q000737:chemistry*; D009344:Neisseria gonorrhoeae / Q000302:isolation & purification; D044367:Phase Transition; D015034:Zinc Oxide / Q000737:chemistry*
D015034:Zinc Oxide