Plasma levels of FL and SDF-1 and expression of FLT-3 and CXCR4 on CD34+ cells assessed pre and post hematopoietic stem cell mobilization in patients with hematologic malignancies and in healthy donors.
Peripheral blood stem cells (PBSC) became the main source of cells for autologous transplantation. Alterations in the expression of adhesion molecules are essential in the CD34+ cells mobilization process. These molecules are involved in the interaction between hematopoietic and stromal cells and they have been disclosed as a considerable factor to the trafficking and homing of the CD34+ progenitor cells. This is a non-randomized PBSC mobilization study designed to evaluate the influence and behavior of FL and SDF-1 and their receptors in two different moments, prior and after HPCs mobilization, with the yield of CD34+ cells collected by apheresis. There was higher concentration of FL and lower of SDF-1 plasma level at post than pre PBSC mobilization (p=0.001 and p=0.012, respectively) regarding all individuals searched, but without any correlation with a good yield of CD34+ cells. However, CXCR4 expressions on the CD34+ cells from bone marrow aspirates (BMA), at pre and post mobilization showed a difference statistical significant for those individuals with good yield of CD34+ cells (p=0,036), but not achieved for poor yield (p=0,156). There was a higher expression of CXCR4 in steady-state for the successfully individuals than for those unsuccessfully (529.84+/-54.68 and 496.31+/-97.51, respectively). In conclusion, we confirmed the important role of CXCR4/SDF-1 axis in the process of PBSC mobilization.
Cecyn
Karin Zattar
KZ
UNIFESP-Universidade Federal de São Paulo, Department of Clinical and Experimental Oncology, Rua Botucatu 740, Blood Bank 4th Floor, CEP-04023-902 São Paulo SP, Brazil. cecyn@uol.com.br
Schimieguel
Dulce Marta
DM
Kimura
Eliza Yuriko Sugano
EY
Yamamoto
Mihoko
M
Oliveira
José Salvador Rodrigues de
JS
D000328:Adult; D018952:Antigens, CD34; D001856:Bone Marrow Examination; D054377:Chemokine CXCL12 / Q000097:blood*; D015536:Down-Regulation; D005260:Female; D019650:Hematopoietic Stem Cell Mobilization; D006412:Hematopoietic Stem Cells / Q000378:metabolism*; D006801:Humans; D008297:Male; D008875:Middle Aged; D036102:Peripheral Blood Stem Cell Transplantation; D019718:Receptors, CXCR4 / Q000097:blood / Q000378:metabolism*; D055815:Young Adult; D051941:fms-Like Tyrosine Kinase 3 / Q000097:blood*
D018952:Antigens, CD34; D054377:Chemokine CXCL12; D019718:Receptors, CXCR4; C497970:FLT3 protein, human; D051941:fms-Like Tyrosine Kinase 3