Pseudomonas aeruginosa Las quorum sensing autoinducer suppresses growth and biofilm production in Legionella species.
Bacteria commonly communicate with each other by a cell-to-cell signalling mechanism known as quorum sensing (QS). Recent studies have shown that the Las QS autoinducer N-(3-oxododecanoyl)-l-homoserine lactone (3-oxo-C(12)-HSL) of Pseudomonas aeruginosa performs a variety of functions not only in intraspecies communication, but also in interspecies and interkingdom interactions. In this study, we report the effects of Pseudomonas 3-oxo-C(12)-HSL on the growth and suppression of virulence factors in other bacterial species that frequently co-exist with Ps. aeruginosa in nature. It was found that 3-oxo-C(12)-HSL, but not its analogues, suppressed the growth of Legionella pneumophila in a dose-dependent manner. However, 3-oxo-C(12)-HSL did not exhibit a growth-suppressive effect on Serratia marcescens, Proteus mirabilis, Escherichia coli, Alcaligenes faecalis and Stenotrophomonas maltophilia. A concentration of 50 microM 3-oxo-C(12)-HSL completely inhibited the growth of L. pneumophila. Additionally, a significant suppression of biofilm formation was demonstrated in L. pneumophila exposed to 3-oxo-C(12)-HSL. Our results suggest that the Pseudomonas QS autoinducer 3-oxo-C(12)-HSL exerts both bacteriostatic and virulence factor-suppressive activities on L. pneumophila alone.
Kimura
Soichiro
S
Department of Microbiology and Infectious Diseases, Toho University Faculty of Medicine, Ota-ku, Tokyo 143-8540, Japan.
Tateda
Kazuhiro
K
Department of Microbiology and Infectious Diseases, Toho University Faculty of Medicine, Ota-ku, Tokyo 143-8540, Japan.
Ishii
Yoshikazu
Y
Department of Microbiology and Infectious Diseases, Toho University Faculty of Medicine, Ota-ku, Tokyo 143-8540, Japan.
Horikawa
Manabu
M
Suntory Institute for Bioorganic Research, Mishima-gun, Osaka 618-8503, Japan.
Miyairi
Shinichi
S
Laboratory of Bio-organic Chemistry, College of Pharmacy, Nihon University, Funabashi, Chiba 274-8555, Japan.
Gotoh
Naomasa
N
Department of Microbiology, Kyoto Pharmaceutical University, Yamashina, Kyoto 607-8414, Japan.
Ishiguro
Masaji
M
Suntory Institute for Bioorganic Research, Mishima-gun, Osaka 618-8503, Japan.
Yamaguchi
Keizo
K
Department of Microbiology and Infectious Diseases, Toho University Faculty of Medicine, Ota-ku, Tokyo 143-8540, Japan.
D015107:4-Butyrolactone / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000378:metabolism; D018441:Biofilms / Q000187:drug effects*; D005798:Genes, Bacterial; D006714:Homoserine / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000378:metabolism; D016952:Legionella pneumophila / Q000187:drug effects* / Q000472:pathogenicity* / Q000502:physiology; D011550:Pseudomonas aeruginosa / Q000378:metabolism*; D053038:Quorum Sensing / Q000235:genetics; D012329:RNA, Bacterial / Q000235:genetics / Q000302:isolation & purification; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014774:Virulence / Q000187:drug effects; D037521:Virulence Factors / Q000378:metabolism; D014871:Water Microbiology
C109860:N-(3-oxododecanoyl)homoserine lactone; C092312:N-butyrylhomoserine lactone; D012329:RNA, Bacterial; D037521:Virulence Factors; D006714:Homoserine; D015107:4-Butyrolactone