title: Utility and evaluation of new variable-number tandem-repeat systems for genotyping mycobacterial tuberculosis isolates.
abstract: We compared mycobacterial interspersed repetitive unit (MIRU)-variable number tandem repeat (VNTR) typing to traditional spoligotyping for discriminating Mycobacterium tuberculosis (MTB) strains. Our 17-loci MIRU-VNTR typing method was found to be superior to spoligotyping for non-Beijing family strains. To extend the method we also established PCR-based rapid genotyping protocols for Beijing, East-African-Indian and U lineages.
authors.affiliation: Division of Clinical Research, National Health Research Institutes, Zhunan, Taiwan.
authors.forename: Horng-Yunn
authors.initials: HY
authors.lastname: Dou
authors.forename: Jang-Jih
authors.initials: JJ
authors.lastname: Lu
authors.forename: Chih-Wei
authors.initials: CW
authors.lastname: Lin
authors.forename: Jia-Ru
authors.initials: JR
authors.lastname: Chang
authors.forename: Jun-Ren
authors.initials: JR
authors.lastname: Sun
authors.forename: Ih-Jen
authors.initials: IJ
authors.lastname: Su
mesh_terms: D015373:Bacterial Typing Techniques / Q000379:methods*; D016172:DNA Fingerprinting / Q000379:methods*; D005838:Genotype; D006801:Humans; D018598:Minisatellite Repeats; D009169:Mycobacterium tuberculosis / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000379:methods; D014397:Tuberculosis, Pulmonary / Q000382:microbiology*