title: IVF outcome in women with endometriosis in relation to tumour necrosis factor and anti-Müllerian hormone.
abstract: This study reports on anti-Müllerian hormone (AMH) in serum and follicular fluid (FF) in relation to inflammatory parameters in women with and without endometriosis undergoing IVF. Serum and FF samples were obtained from 72 women, with (n = 34) and without (n = 38) endometriosis, undergoing IVF. The concentrations of AMH, FSH, tumour necrosis factor (TNF), granulocyte-macrophage colony-stimulating factor (GM-CSF), vascular endothelial growth factor (VEGF) and several interleukins were analysed. Women with endometriosis had significantly lower AMH in serum and FF (serum: 6.38 versus 12.8 pM; P < 0.01, FF: 14.0 versus 19.6 pM; P < 0.05). TNF was increased in FF (40.0 versus 30.8 pg/ml, P < 0.05) from women with endometriosis and significantly higher concentrations of IL-15 and GM-CSF were detected in FF (both P < 0.05). During IVF, women with endometriosis responded well to FSH but had lower fertilization rates. Women with endometriosis have elevated concentrations of several cytokines in FF. They respond adequately to exogenous FSH but may have impaired oocyte quality, reflected in lower fertilization rates, presumably resulting from an inflammatory process in the ovaries. Further studies are needed to elucidate the role of AMH in predicting ovarian reserve in women with endometriosis.
authors.affiliation: Department of Woman and Child Health, Division of Obstetrics and Gynecology, Karolinska Institutet/Karolinska University Hospital, Stockholm, Sweden. henrik.falconer@karolinska.se
authors.forename: H
authors.initials: H
authors.lastname: Falconer
authors.forename: J
authors.initials: J
authors.lastname: Sundqvist
authors.forename: K
authors.initials: K
authors.lastname: Gemzell-Danielsson
authors.forename: B
authors.initials: B
authors.lastname: von Schoultz
authors.forename: T M
authors.initials: TM
authors.lastname: D'Hooghe
authors.forename: G
authors.initials: G
authors.lastname: Fried
mesh_terms: D000328:Adult; D054304:Anti-Mullerian Hormone / Q000032:analysis / Q000097:blood; D016207:Cytokines / Q000032:analysis; D004715:Endometriosis / Q000097:blood* / Q000378:metabolism*; D005260:Female; D005307:Fertilization in Vitro / Q000379:methods*; D005640:Follicle Stimulating Hormone / Q000032:analysis / Q000097:blood; D015571:Follicular Fluid / Q000737:chemistry*; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000032:analysis / Q000097:blood; D006801:Humans; D011247:Pregnancy; D011256:Pregnancy Outcome; D018709:Statistics, Nonparametric; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis / Q000097:blood; D042461:Vascular Endothelial Growth Factor A / Q000032:analysis / Q000097:blood
chemical_list: D016207:Cytokines; D014409:Tumor Necrosis Factor-alpha; D042461:Vascular Endothelial Growth Factor A; D054304:Anti-Mullerian Hormone; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D005640:Follicle Stimulating Hormone