BS69 negatively regulates the canonical NF-kappaB activation induced by Epstein-Barr virus-derived LMP1.
Epstein-Barr virus (EBV) latent membrane protein 1 (LMP1) activates NF-kappaB signaling pathways through the two C-terminal regions, CTAR1 and CTAR2. BS69 has previously been shown to be involved in LMP1-induced c-Jun N-terminal kinase activation through CTAR2 by interacting with tumor necrosis factor (TNFR) receptor-associated factor 6. In the present study, our manipulation of BS69 expression clearly indicates that BS69 negatively regulates LMP1-mediated NF-kappaB activation and up-regulates IL-6 mRNA expression and IkappaB degradation. Our immunoprecipitation experiments suggest that BS69 decreases complex formation between LMP1 and TNFR-associated death domain protein (TRADD).
Ikeda
Osamu
O
Department of Immunology, Graduate School of Pharmaceutical Sciences, Hokkaido University, Kita-Ku Kita 12 Nishi 6, Sapporo, Japan.
Sekine
Yuichi
Y
Mizushima
Akihiro
A
Oritani
Kenji
K
Yasui
Teruhito
T
Fujimuro
Masahiro
M
Muromoto
Ryuta
R
Nanbo
Asuka
A
Matsuda
Tadashi
T
D002352:Carrier Proteins / Q000032:analysis / Q000378:metabolism*; D018797:Cell Cycle Proteins; D002478:Cells, Cultured; D056970:Co-Repressor Proteins; D004268:DNA-Binding Proteins; D005786:Gene Expression Regulation; D006367:HeLa Cells; D006801:Humans; D015850:Interleukin-6 / Q000235:genetics / Q000378:metabolism; D008856:Microscopy, Fluorescence; D008954:Models, Biological; D016328:NF-kappa B / Q000032:analysis / Q000378:metabolism*; D015398:Signal Transduction; D053199:TNF Receptor-Associated Death Domain Protein / Q000378:metabolism; D014763:Viral Matrix Proteins / Q000032:analysis / Q000378:metabolism*
D002352:Carrier Proteins; D018797:Cell Cycle Proteins; D056970:Co-Repressor Proteins; D004268:DNA-Binding Proteins; C037662:EBV-associated membrane antigen, Epstein-Barr virus; D015850:Interleukin-6; D016328:NF-kappa B; D053199:TNF Receptor-Associated Death Domain Protein; D014763:Viral Matrix Proteins; C094763:ZMYND11 protein, human