title: Simultaneous quantification of 19 ginsenosides in black ginseng developed from Panax ginseng by HPLC-ELSD.
abstract: A high-performance liquid chromatographic method with evaporative light scattering detection (HPLC-ELSD) has been developed to identify and quantify 19 ginsenosides (Rg(1), Re, Rf, Rb(1), Rc, Rb(2), Rd, F(4), Rg(6), Rk(3), Rh(4), 20(S)-, 20(R)-Rg(3), 20(S)-, 20(R)-Rs(3), Rk(1), Rg(5), Rs(4), and Rs(5)) in black ginseng (BG, Korean white ginseng that was subjected to nine cycles of steam treatment). Ultrasonication is employed for sample preparation, and the analysis is achieved on a Discovery C(18) column using gradient elution of CH(3)CN-H(2)O-CH(3)COOH without buffer in 40min. The method was validated by linearity (r(2)> or =0.9994), precision (92.0-107.5%), intra- and inter-day accuracy (R.S.D.<3.21%), and limit of detection (LOD< or =93ng). The quantification method was applied to analyze the composition of ginsenosides in Korean white, red, and black ginsengs. During the preparatory process of BG, ginsenosides transform into constituents of low polarity by hydrolysis, isomerization, and dehydration at C-20, and hydrolysis also occurs at C-3 or C-6. The validated HPLC method is expected to provide the basis for the quality assessment of ginseng products.
authors.affiliation: Department of Food Science and Technology, Chungnam National University, Yusung-Gu, Taejeon, South Korea.
authors.forename: Bai-Shen
authors.initials: BS
authors.lastname: Sun
authors.forename: Li-Juan
authors.initials: LJ
authors.lastname: Gu
authors.forename: Zhe-Ming
authors.initials: ZM
authors.lastname: Fang
authors.forename: Chun-yan
authors.initials: CY
authors.lastname: Wang
authors.forename: Zhen
authors.initials: Z
authors.lastname: Wang
authors.forename: Mi-Ra
authors.initials: MR
authors.lastname: Lee
authors.forename: Zheng
authors.initials: Z
authors.lastname: Li
authors.forename: Jing-Jie
authors.initials: JJ
authors.lastname: Li
authors.forename: Chang-Keun
authors.initials: CK
authors.lastname: Sung
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D036145:Ginsenosides / Q000032:analysis*; D005894:Panax / Q000737:chemistry*; D015203:Reproducibility of Results
chemical_list: D036145:Ginsenosides