title: [Studies on application of ultra-filtration to purifying polysaccharides from Rhubarb].
abstract: OBJECTIVE
  To purify Rhubarb polysaccharides via micro-filtration and ultra-filtration.
  
  
  METHODS
  The technology adopted micro-filtration and tubular membrane with different cut-off molecular weights.
  
  
  RESULTS
  Experiment results showed that the optimum operating conditions were that the ultra-filtration time controlled around 80 min, the temperature was set the 35-40 degrees C, the operating pressure was 0.08-0.12 MPa, the pH was 6-8, and a sample that of polysaccharide was 0.5 times of original concentration. The recovery ratio of the polysaccharides was 53.7%, the concentration could be condensed to 2.73 times compared with the sample liquid.
  
  
  CONCLUSIONS
  The technology was simple and feasible, it can be applied to purify Rhubarb polysaccharides.
authors.affiliation: Modern Engineering Center for Chinese Traditional Medicine, East China University of Science and Technology, Shanghai 200237, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Sun
authors.forename: Yongchuan
authors.initials: Y
authors.lastname: Zhou
authors.forename: Dexu
authors.initials: D
authors.lastname: Chen
mesh_terms: D005240:Feasibility Studies; D006863:Hydrogen-Ion Concentration; D011134:Polysaccharides / Q000302:isolation & purification*; D011312:Pressure; D012250:Rheum / Q000737:chemistry*; D013696:Temperature; D013997:Time Factors; D014462:Ultrafiltration / Q000379:methods*
chemical_list: D011134:Polysaccharides