title: Ca2+ increases the specific coenzyme Q10 content in Agrobacterium tumefaciens.
abstract: Of various metal ions (Ca2+, Cr3+, Cu2+, Fe2+, Mg2+, Mn2+, Ni2+ and Zn2+) added to the culture medium of Agrobacterium tumefaciens at 1 mM, only Ca2+ increased Coenzyme Q10 (CoQ(10)) content in cells without the inhibition of cell growth. In a pH-stat fed-batch culture, supplementation with 40 mM of CaCO3 increased the specific CoQ(10) content and oxidative stress by 22.4 and 48%, respectively. Also, the effect of Ca2+ on the increase of CoQ(10) content was successfully verified in a pilot-scale (300 L) fermentor. In this study, the increased oxidative stress in A. tumefaciens culture by the supplementation of Ca2+ is hypothesized to stimulate the increase of specific CoQ(10) content in order to protect the membrane against lipid peroxidation. Our results improve the understanding of Ca2+ effect on CoQ(10) biosynthesis in A. tumefaciens and should contribute to better industrial production of CoQ(10) by biological processes.
authors.affiliation: BioNgene Co., Ltd., 10-1 Myungryun-Dong Chongro-Ku, Seoul, 110-521, Korea.
authors.forename: Suk-Jin
authors.initials: SJ
authors.lastname: Ha
authors.forename: Sang-Yong
authors.initials: SY
authors.lastname: Kim
authors.forename: Jin-Ho
authors.initials: JH
authors.lastname: Seo
authors.forename: Marimuthu
authors.initials: M
authors.lastname: Jeya
authors.forename: Ye-Wang
authors.initials: YW
authors.lastname: Zhang
authors.forename: Thangadurai
authors.initials: T
authors.lastname: Ramu
authors.forename: In-Won
authors.initials: IW
authors.lastname: Kim
authors.forename: Jung-Kul
authors.initials: JK
authors.lastname: Lee
mesh_terms: D016960:Agrobacterium tumefaciens / Q000187:drug effects / Q000378:metabolism*; D002118:Calcium / Q000008:administration & dosage*; D004305:Dose-Response Relationship, Drug; D014451:Ubiquinone / Q000031:analogs & derivatives* / Q000378:metabolism
chemical_list: D014451:Ubiquinone; C024989:coenzyme Q10; D002118:Calcium