Cordycepin inhibits UVB-induced matrix metalloproteinase expression by suppressing the NF-kappaB pathway in human dermal fibroblasts.
Cordycepin (3-deoxyadenosine) has been shown to exhibit many pharmacological activities, including anti-cancer, anti-inflammatory, and anti-infection activities. However, the anti-skin photoaging effects of cordycepin have not yet been reported. In the present study, we investigated the inhibitory effects of cordycepin on matrix metalloproteinase-1 (MMP-1) and -3 expressions of the human dermal fibroblast cells. Western blot analysis and real-time PCR revealed cordycepin inhibited UVB-induced MMP-1 and -3 expressions in a dose-dependent manner. UVB strongly activated NF-kappaB activity, which was determined by IkappaBalpha degradation, nuclear localization of p50 and p65 subunit, and NF-kappaB binding activity. However, UVB-induced NF-kappaB activation and MMP expression were completely blocked by cordycepin pretreatment. These findings suggest that cordycepin could prevent UVB-induced MMPs expressions through inhibition of NF-kappaB activation. In conclusion, cordycepin might be used as a potential agent for the prevention and treatment of skin photoaging.
Lee
Young Rae
YR
Department of Biochemistry and Institute for Medical Sciences Korea, Chonbuk National University Medical School, Jeonju 560-182, Korea.
Noh
Eun Mi
EM
Jeong
Eun Yong
EY
Yun
Seok Kweon
SK
Jeong
Young Ju
YJ
Kim
Jong Hyeon
JH
Kwon
Kang Beom
KB
Kim
Byeong Soo
BS
Lee
Sung Ho
SH
Park
Chang Sik
CS
Kim
Jong Suk
JS
D000375:Aging / Q000502:physiology; D002478:Cells, Cultured; D003839:Deoxyadenosines / Q000494:pharmacology*; D020405:Dermis / Q000166:cytology / Q000187:drug effects / Q000502:physiology / Q000528:radiation effects; D004305:Dose-Response Relationship, Drug; D004790:Enzyme Induction / Q000187:drug effects; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects; D015971:Gene Expression Regulation, Enzymologic; D006801:Humans; D007231:Infant, Newborn; D008297:Male; D020781:Matrix Metalloproteinase 1 / Q000096:biosynthesis / Q000235:genetics / Q000528:radiation effects; D019278:Matrix Metalloproteinase 3 / Q000096:biosynthesis* / Q000235:genetics / Q000528:radiation effects; D061965:Matrix Metalloproteinase Inhibitors; D016328:NF-kappa B / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000378:metabolism; D012867:Skin / Q000503:physiopathology / Q000528:radiation effects; D014466:Ultraviolet Rays
D003839:Deoxyadenosines; D061965:Matrix Metalloproteinase Inhibitors; D016328:NF-kappa B; C511768:MMP3 protein, human; D019278:Matrix Metalloproteinase 3; C522360:MMP1 protein, human; D020781:Matrix Metalloproteinase 1; C058120:cordycepin