Chemical composition and biological activities of essential oil from Salvia sclarea plants regenerated in vitro.
The essential oils obtained by hydrodistillation of dried aerial parts of Salvia sclarea L. plants, regenerated in vitro and reproduced from seeds, were analyzed by GC and GC-MS. The oils from in vitro and in vivo plants were compared in respect to their chemical composition as well as antimicrobial and cytotoxic activities. The chemical profiles of both oils were very similar, although the yield of essential oil from in vitro plants was lower (0.1%, v/w) than the oil yield isolated from in vivo S. sclarea plants (0.2%, v/w). Both oils showed antimicrobial and cytotoxic activity. The oil from in vitro regenerated plants of S. sclarea exhibited stronger cytotoxic action against NALM-6 cell lines in comparison with the essential oil from in vivo plants.
Kuźma
Lukasz
L
Department of Biology and Pharmaceutical Botany, Medical University of Łódź, Łódź, Poland. lukikuzma@wp.pl
Kalemba
Danuta
D
Rózalski
Marek
M
Rózalska
Barbara
B
Wieckowska-Szakiel
Marzena
M
Krajewska
Urszula
U
Wysokińska
Halina
H
D045744:Cell Line, Tumor / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D006801:Humans; D008826:Microbial Sensitivity Tests; D009822:Oils, Volatile / Q000737:chemistry* / Q000494:pharmacology; D010938:Plant Oils / Q000737:chemistry* / Q000494:pharmacology; D012038:Regeneration / Q000502:physiology*; D027544:Salvia / Q000033:anatomy & histology / Q000737:chemistry* / Q000502:physiology*
D009822:Oils, Volatile; D010938:Plant Oils