title: Evidence for pseudoknot formation of class I preQ1 riboswitch aptamers.
abstract: All in a knot. The smallest riboswitch forms a pseudoknot in solution. This is demonstrated for preQ(1) class I aptamers by mutational analysis in combination with (1)H NMR-based structure probing. How pseudoknot formation mediates the mRNA response through its expression platform is now open for investigation.
authors.affiliation: Institute of Organic Chemistry, Center for Molecular Biosciences CMBI, 6020 Innsbruck. Austria.
authors.forename: Ulrike
authors.initials: U
authors.lastname: Rieder
authors.forename: Kathrin
authors.initials: K
authors.lastname: Lang
authors.forename: Christoph
authors.initials: C
authors.lastname: Kreutz
authors.forename: Norbert
authors.initials: N
authors.lastname: Polacek
authors.forename: Ronald
authors.initials: R
authors.lastname: Micura
mesh_terms: D020121:5' Untranslated Regions; D052157:Aptamers, Nucleotide / Q000737:chemistry* / Q000378:metabolism; D020029:Base Pairing; D001483:Base Sequence; D016967:Fusobacterium nucleatum / Q000235:genetics / Q000378:metabolism; D009154:Mutation; D009690:Nucleic Acid Conformation; D011744:Pyrimidinones / Q000737:chemistry* / Q000378:metabolism; D011758:Pyrroles / Q000737:chemistry* / Q000378:metabolism; D012329:RNA, Bacterial / Q000737:chemistry / Q000378:metabolism; D012343:RNA, Transfer / Q000737:chemistry / Q000378:metabolism; D038621:Regulatory Sequences, Ribonucleic Acid
chemical_list: D020121:5' Untranslated Regions; C096876:7-(aminomethyl)-7-deazaguanine; D052157:Aptamers, Nucleotide; D011744:Pyrimidinones; D011758:Pyrroles; D012329:RNA, Bacterial; D038621:Regulatory Sequences, Ribonucleic Acid; D012343:RNA, Transfer