High-throughput analysis of serum antigens using sandwich ELISAs on microarrays.
Enzyme-linked immunosorbent assay (ELISA) microarrays promise to be a powerful tool for the detection and validation of disease biomarkers. ELISA microarrays are capable of simultaneous detection of many proteins using a small sample volume. Although there are many potential pitfalls to the use of ELISA microarrays, these can be avoided by careful planning of experiments. In this chapter we describe a high-throughput protocol for processing ELISA microarrays that will result in reliable and reproducible data.
Servoss
Shannon L
SL
Biological Sciences Division, Pacific Northwest National Laboratory, Richland, WA, USA.
Gonzalez
Rachel
R
Varnum
Susan
S
Zangar
Richard C
RC
D000941:Antigens / Q000097:blood*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006801:Humans; D040081:Protein Array Analysis / Q000379:methods*
D000941:Antigens