A rapid microwave-assisted derivatization of bacterial metabolome samples for gas chromatography/mass spectrometry analysis.
Analysis of metabolome samples by gas chromatography/mass spectrometry requires a comprehensive derivatization method to afford quantitative and qualitative information of a complex biological sample. Here we describe an extremely time-effective microwave-assisted protocol for the commonly used methoxyamine and N-methyl-N-trimethylsilylfluoracetamide silylation method of primary metabolites. Our studies show that microwave irradiation can decrease the sample preparation time from approximately 120 min to 6 min without loss of either qualitative or quantitative information for the tested synthetic metabolite mixtures and microbial-derived metabolome samples collected from Bacillus subtilis and Staphylococcus aureus. Comparisons of metabolic fingerprints and selected metabolites show no noticeable differences compared with the commonly used heating block methods.
Liebeke
Manuel
M
Institute of Pharmaceutical Biology, Ernst Moritz Arndt University of Greifswald, D-17487 Greifswald, Germany.
Wunder
Ariane
A
Lalk
Michael
M
D001412:Bacillus subtilis / Q000378:metabolism*; D008401:Gas Chromatography-Mass Spectrometry / Q000191:economics / Q000379:methods*; D055442:Metabolome; D055432:Metabolomics / Q000191:economics / Q000379:methods*; D008872:Microwaves; D013211:Staphylococcus aureus / Q000378:metabolism*; D013997:Time Factors