A 96-well format for a high-throughput baculovirus generation, fast titering and recombinant protein production in insect and mammalian cells.
BACKGROUND
Baculovirus expression vector system (BEVS) has become a standard in recombinant protein production and virus-like particle preparation for numerous applications.


RESULTS
We describe here protocols which adapt baculovirus generation into 96-well format.


CONCLUSIONS
The established methodology allows simple baculovirus generation, fast virus titering within 18 h and efficient recombinant protein production in a high-throughput format. Furthermore, the produced baculovirus vectors are compatible with gene expression in vertebrate cells in vitro and in vivo.
Kärkkäinen
Hanna-Riikka
HR
Department of Biotechnology and Molecular Medicine, A.I. Virtanen Institute for Molecular Sciences, Kuopio, Finland. hanna-riikka.karkkainen@arktherapeutics.com
Lesch
Hanna P
HP
Määttä
Antti I
AI
Toivanen
Pyry I
PI
Mähönen
Anssi J
AJ
Roschier
Miia M
MM
Airenne
Kari J
KJ
Laitinen
Olli H
OH
Ylä-Herttuala
Seppo
S