title: Evidence of circulation of Laguna Negra-like hantavirus in the Central West of Brazil: case report.
abstract: BACKGROUND
  Hantavirus pulmonary syndrome has been reported with increasing frequency in some Brazilian regions, but information about viral genetic identification is still limited. Recently, the state of Mato Grosso, in the Legal Amazon of Brazil, experienced a growing number of hantavirus pulmonary syndrome (HPS) cases but the genetic characterization of the causative hantavirus is still missing.
  
  
  OBJECTIVE
  Our goal was to identify the hantavirus strain involved in a fatal HPS case in the Central region of Brazil.
  
  
  METHODS
  Nested RT-PCR was conducted on blood clot samples from an HPS patient from Mato Grosso. PCR-positive samples were sequenced, and the resulting sequences were compared with reference samples. Viral antigens were detected by immunohistological analyses in lung and liver tissues.
  
  
  RESULTS
  Analyses of the viral RNA isolated from the patient identified a Laguna Negra (LN)-like virus as the causative agent and histological analysis of lung sections were compatible with the genetic characterization.
  
  
  CONCLUSIONS
  This is the first report of circulation and human infection by a Laguna Negra-like hantavirus in Brazil.
authors.affiliation: Instituto Carlos Chagas, ICC/Fiocruz/PR, Brazil.
authors.forename: Sonia Mara
authors.initials: SM
authors.lastname: Raboni
authors.forename: Luana
authors.initials: L
authors.lastname: de Borba
authors.forename: Federico G
authors.initials: FG
authors.lastname: Hoffmann
authors.forename: Lucia
authors.initials: L
authors.lastname: de Noronha
authors.forename: Marin Luize Viola
authors.initials: ML
authors.lastname: Azevedo
authors.forename: Suzana
authors.initials: S
authors.lastname: Carstensen
authors.forename: Giovanny A C A
authors.initials: GA
authors.lastname: Mazzarotto
authors.forename: Juliano
authors.initials: J
authors.lastname: Bordignon
authors.forename: Claudia Nunes Duarte
authors.initials: CN
authors.lastname: dos Santos
mesh_terms: D000328:Adult; D001938:Brazil; D017809:Fatal Outcome; D006476:Orthohantavirus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D018778:Hantavirus Infections / Q000175:diagnosis* / Q000821:virology*; D006801:Humans; D008099:Liver / Q000473:pathology / Q000821:virology; D008168:Lung / Q000473:pathology / Q000821:virology; D008297:Male; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012367:RNA, Viral / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D055815:Young Adult
chemical_list: D012367:RNA, Viral