Development and validation of a quantitative method for the determination of 12 endocannabinoids and related compounds in human plasma using liquid chromatography-tandem mass spectrometry.
A sensitive and specific LC-MS/MS method for the quantification of the endocannabinoids and related structures anandamide, 2-arachidonoyl glycerol, 2-arachidonyl glycerol ether, O-arachidonoyl ethanolamide, dihomo-gamma-linolenoyl ethanolamide, docosatetraenoyl ethanolamide, N-arachidonoyl dopamine, N-arachidonyl glycine, N-oleoyl dopamine, oleoyl ethanolamide, palmitoyl ethanolamide, and stearoyl ethanolamide in human plasma was developed and validated. Compounds were extracted using acetonitrile followed by solid-phase extraction. Separation was performed on a Xterra C8 column using gradient elution coupled to a triple-quadrupole MS. LLOQ levels ranged from 0.02 to 1.75 microg/mL, LODs ranged from 0.0002 to 0.1266 ng/mL, and accuracies were >80% (except stearoyl ethanolamide at lowest spike level) at all spike levels.
Balvers
Michiel G J
MG
TNO Quality of Life, Departments of Analytical Research and Biosciences, AJ Zeist, The Netherlands. Michiel.Balvers@tno.nl
Verhoeckx
Kitty C M
KC
Witkamp
Renger F
RF
D063385:Cannabinoid Receptor Modulators / Q000097:blood / Q000737:chemistry*; D002853:Chromatography, Liquid / Q000379:methods*; D063388:Endocannabinoids; D006801:Humans; D053719:Tandem Mass Spectrometry / Q000379:methods*
D063385:Cannabinoid Receptor Modulators; D063388:Endocannabinoids