Synthesis, characterization, DNA-binding and antiproliferative activity of Nd(III) complexes with N-(nitrogen heterocyclic) norcantharidin acylamide acid.
Three novel complexes [Nd(L)(NO3)(H2O)2].NO(3).2H2O (HL1 = N-pyrimidine norcantharidin acylamide acid, C12H13N3O4; HL2 = N-pyridine norcantharidin acylamide acid, C13H14N2O4; HL3 = N-phenyl norcantharidin acylamide acid, C14H15NO4) were synthesized. HL1, HL2 and HL3 are the ligand of complex(1), complex(2) and complex(3), respectively. Their structures were characterized by elemental analysis, conductivity measurement, infrared spectra and thermogravimetric analysis. The DNA-binding properties of the complexes have been investigated by fluorescence spectroscopy and viscosity measurements. The results suggest that the complexes can bind to DNA by partial intercalation. The liner Stern-Volmer quenching constant Ksq values are 3.3(+/-0.21)(1), 1.7(+/-0.19)(2) and 0.9(+/-0.04)(3), respectively. Complex (1) and (2) have been found to cleave pBR322 plasmid DNA at physiological pH and temperature. The test of antiproliferation activity indicates that complex(1) has strong antiproliferative ability against the SMMC7721 (IC50 = 131.7 +/- 23.4 micromol x L(-1)) and A549 (IC50 = 128.4 +/- 19.9 micromol x L(-1)) cell lines. The inhibition rates of complex(2) (IC50 = 86.3 +/- 11.3 micromol x L(-1)) are much higher than that of NCTD (IC50 = 115.5 +/- 9.5 micromol x L(-1)) and HL2 (111.0 +/- 5.7 micromol x L(-1)) against SMMC7721 cell lines.
Zhu
Wen-Zhong
WZ
Zhejiang Key Laboratory for Reaction Chemistry on Solid Surfaces, Zhejiang Normal University, Jinhua 321004, China.
Lin
Qiu-Yue
QY
Lu
Mei
M
Hu
Rui-Ding
RD
Zheng
Xiao-Liang
XL
Cheng
Jian-Ping
JP
Wang
Yun-Yun
YY
D000577:Amides / Q000737:chemistry*; D000818:Animals; D019086:Bridged Bicyclo Compounds, Heterocyclic / Q000737:chemistry*; D002417:Cattle; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D004247:DNA / Q000737:chemistry*; D004354:Drug Screening Assays, Antitumor; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008024:Ligands; D009354:Neodymium / Q000737:chemistry*; D009942:Organometallic Compounds / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology*; D013696:Temperature
D000577:Amides; D019086:Bridged Bicyclo Compounds, Heterocyclic; D008024:Ligands; D009942:Organometallic Compounds; D009354:Neodymium; C069741:norcantharidin; D004247:DNA; C427203:calf thymus DNA