title: Rapid group-, serotype-, and vaccine strain-specific identification of poliovirus isolates by real-time reverse transcription-PCR using degenerate primers and probes containing deoxyinosine residues.
abstract: We have adapted our previously described poliovirus diagnostic reverse transcription-PCR (RT-PCR) assays to a real-time RT-PCR (rRT-PCR) format. Our highly specific assays and rRT-PCR reagents are designed for use in the WHO Global Polio Laboratory Network for rapid and large-scale identification of poliovirus field isolates.
authors.affiliation: Polio and Picornavirus Laboratory Branch, G-10, Division of Viral Diseases, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA. DKilpatrick@cdc.gov
authors.forename: David R
authors.initials: DR
authors.lastname: Kilpatrick
authors.forename: Chen-Fu
authors.initials: CF
authors.lastname: Yang
authors.forename: Karen
authors.initials: K
authors.lastname: Ching
authors.forename: Annelet
authors.initials: A
authors.lastname: Vincent
authors.forename: Jane
authors.initials: J
authors.lastname: Iber
authors.forename: Ray
authors.initials: R
authors.lastname: Campagnoli
authors.forename: Mark
authors.initials: M
authors.lastname: Mandelbaum
authors.forename: Lina
authors.initials: L
authors.lastname: De
authors.forename: Su-Ju
authors.initials: SJ
authors.lastname: Yang
authors.forename: Allan
authors.initials: A
authors.lastname: Nix
authors.forename: Olen M
authors.initials: OM
authors.lastname: Kew
mesh_terms: D017931:DNA Primers / Q000235:genetics*; D006801:Humans; D015345:Oligonucleotide Probes / Q000235:genetics*; D017955:Poliovirus / Q000145:classification* / Q000302:isolation & purification*; D012367:RNA, Viral / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity
chemical_list: D017931:DNA Primers; D015345:Oligonucleotide Probes; D012367:RNA, Viral