Exon junction complex enhances translation of spliced mRNAs at multiple steps.
Translation of spliced mRNAs is enhanced by exon junction complex (EJC), which is deposited on mRNAs as a result of splicing. Although this phenomenon itself is well known, the underlying molecular mechanism remains poorly understood. Here we show, using siRNAs against Y14 and eIF4AIII and spliced or intronless constructs that contain different types of internal ribosome entry sites (IRESes), that Y14 and eIF4AIII increase translation of spliced mRNAs before and after formation of the 80S ribosome complex, respectively. These results suggest that EJC modulates translation of spliced mRNA at multiple steps.
Lee
Hyung Chul
HC
School of Life Sciences and Biotechnology, Korea University, Seoul 136-701, Republic of Korea.
Choe
Junho
J
Chi
Sung-Gil
SG
Kim
Yoon Ki
YK
D039601:Eukaryotic Initiation Factor-4A / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D005091:Exons; D006367:HeLa Cells; D006801:Humans; D014176:Protein Biosynthesis / Q000235:genetics*; D012326:RNA Splicing; D012333:RNA, Messenger / Q000235:genetics*; D034741:RNA, Small Interfering / Q000235:genetics; D016601:RNA-Binding Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D012270:Ribosomes / Q000378:metabolism
C406243:RBM8A protein, human; D012333:RNA, Messenger; D034741:RNA, Small Interfering; D016601:RNA-Binding Proteins; D039601:Eukaryotic Initiation Factor-4A