title: Cellular mRNA expressions of anti-oxidant factors in the blood of preeclamptic women.
abstract: OBJECTIVE
  To assess the alterations of mRNA expressions associated with oxidative stress in the cellular component of blood from pregnant women with pre-eclampsia.
  
  
  METHODS
  Peripheral blood samples were obtained from pregnant women with and without pre-eclampsia. Cellular RNA was subjected to a reverse transcription polymerase chain reaction (PCR) assay in order to examine the mRNA distribution among women with pre-eclampsia (n = 24) and control subjects (n = 24) during 35-41 weeks of gestation. The data were analyzed by non-parametric statistics.
  
  
  RESULTS
  Significant differences between the pre-eclampsia subjects and the controls were observed in the gene expressions associated with oxidative stress. Lower values in the pre-eclampsia group were found for heme oxygenase (HO)-1, HO-2, catalase and superoxide dismutase (SOD). The HO-1, HO-2 and the catalase levels significantly correlated with proteinuria, and the HO-2 level with systolic blood pressure.
  
  
  CONCLUSIONS
  Significantly lower concentrations of HO-1, HO-2, SOD and catalase are found in the cellular component of blood from pre-eclamptic patients. The values correlate with the severity of pre-eclampsia. These findings indicate that enhanced oxidative stress and a decrease in the number of anti-oxidant enzymes may be associated with pre-eclampsia.
authors.affiliation: Department of Obstetrics and Gynecology, Showa University School of Medicine, Tokyo, Japan.
authors.forename: Masamitsu
authors.initials: M
authors.lastname: Nakamura
authors.forename: Akihiko
authors.initials: A
authors.lastname: Sekizawa
authors.forename: Yuditiya
authors.initials: Y
authors.lastname: Purwosunu
authors.forename: Shiho
authors.initials: S
authors.lastname: Okazaki
authors.forename: Antonio
authors.initials: A
authors.lastname: Farina
authors.forename: Noroyono
authors.initials: N
authors.lastname: Wibowo
authors.forename: Hanako
authors.initials: H
authors.lastname: Shimizu
authors.forename: Takashi
authors.initials: T
authors.lastname: Okai
mesh_terms: D000293:Adolescent; D000328:Adult; D000975:Antioxidants / Q000378:metabolism*; D001773:Blood Cells / Q000378:metabolism*; D001794:Blood Pressure; D002374:Catalase / Q000235:genetics / Q000378:metabolism; D005260:Female; D017359:HELLP Syndrome / Q000097:blood / Q000235:genetics / Q000378:metabolism / Q000503:physiopathology; D006419:Heme Oxygenase (Decyclizing) / Q000235:genetics / Q000378:metabolism; D051547:Heme Oxygenase-1 / Q000235:genetics / Q000378:metabolism; D006801:Humans; D018384:Oxidative Stress / Q000235:genetics / Q000502:physiology; D011225:Pre-Eclampsia / Q000097:blood* / Q000235:genetics* / Q000378:metabolism / Q000503:physiopathology; D011247:Pregnancy; D012333:RNA, Messenger / Q000097:blood* / Q000378:metabolism; D013482:Superoxide Dismutase / Q000235:genetics / Q000378:metabolism; D055815:Young Adult
chemical_list: D000975:Antioxidants; D012333:RNA, Messenger; D002374:Catalase; D006419:Heme Oxygenase (Decyclizing); D051547:Heme Oxygenase-1; C105245:heme oxygenase-2; D013482:Superoxide Dismutase