title: [Effect of Shuanghuanglian injection on cerebral nuclear factor-kappaB expression in mice with viral encephalitis].
abstract: OBJECTIVE
  To observe the effect of Shuanghuanglian injection on cerebral expression of nuclear factor-kappaB (NF-kappaB) in mice with viral encephalitis.
  
  
  METHODS
  The mice with experimental viral encephalitis received treatment with Shuanghuanglian injection at the dose of 0.2, 1.5, and 5 for 5, 10 or 20 consecutive days. The total RNA of the brain tissue was extracted to analyze the protein and mRNA expression of NF-kappaB using Western blotting and RT-PCR, respectively.
  
  
  RESULTS
  Compared with the control group, the mice with experimental viral encephalitis showed significantly increased protein and mRNA expressions of NF-kappaB (P<0.01). Treatment with Shuanghuanglian injection at the doses of 0.2 and 1.5 mg/kg significantly lowered NF-kappaB protein and mRNA expressions in the brain of mice with viral encephalitis (P<0.05), and the effect was even more obvious at the dose of 5 mg/kg (P<0.01).
  
  
  CONCLUSIONS
  Shuanghuanglian injection can reduce the expression of NF-kappaB in the brain of mice with viral encephalitis in a dose- and time-dependent manner.
authors.affiliation: Department Neurology, Xi'an Central Hospital, Xi'an 710003 China.
authors.forename: Ye
authors.initials: Y
authors.lastname: Tian
authors.forename: Zheng-li
authors.initials: ZL
authors.lastname: DI
authors.forename: Hui
authors.initials: H
authors.lastname: Lei
authors.forename: Ge-juan
authors.initials: GJ
authors.lastname: Zhang
authors.forename: Hua-di
authors.initials: HD
authors.lastname: Liang
authors.forename: Hui-ling
authors.initials: HL
authors.lastname: Chen
mesh_terms: D000818:Animals; D015153:Blotting, Western; D001921:Brain / Q000187:drug effects* / Q000378:metabolism; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage* / Q000494:pharmacology* / Q000627:therapeutic use; D018792:Encephalitis, Viral / Q000188:drug therapy / Q000235:genetics / Q000378:metabolism*; D005786:Gene Expression Regulation / Q000187:drug effects*; D007267:Injections; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D016328:NF-kappa B / Q000235:genetics* / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D004365:Drugs, Chinese Herbal; D016328:NF-kappa B; D012333:RNA, Messenger; C516400:shuang-huang-lian