title: Loop-mediated isothermal amplification (LAMP): a rapid, accurate, and cost-effective diagnostic method for infectious diseases.
abstract: Loop-mediated isothermal amplification (LAMP) is an established nucleic acid amplification method offering rapid, accurate, and cost-effective diagnosis of infectious diseases. This technology has been developed into commercially available detection kits for a variety of pathogens including bacteria and viruses. The current focus on LAMP methodology is as a diagnostic system to be employed in resource-limited laboratories in developing countries, where many fatal tropical diseases are endemic. The combination of LAMP and novel microfluidic technologies such as Lab-on-a-chip may facilitate the realization of genetic point-of-care testing systems to be used by both developed and developing countries in the near future. This review will describe the historical, current, and future developments of such technologies.
authors.affiliation: Biochemical Research Laboratory, Research and Development Division, Eiken Chemical Co., Ltd., 1381-3 Shimoishigami, Ohtawara, Tochigi, 324-0036, Japan. Yasuyoshi_Mori@eiken.co.jp
authors.forename: Yasuyoshi
authors.initials: Y
authors.lastname: Mori
authors.forename: Tsugunori
authors.initials: T
authors.lastname: Notomi
mesh_terms: D000818:Animals; D001419:Bacteria / Q000235:genetics / Q000302:isolation & purification; D003141:Communicable Diseases / Q000175:diagnosis*; D056890:Eukaryota / Q000235:genetics / Q000302:isolation & purification; D006801:Humans; D021141:Nucleic Acid Amplification Techniques / Q000191:economics / Q000379:methods; D019095:Point-of-Care Systems; D013997:Time Factors; D014780:Viruses / Q000235:genetics / Q000302:isolation & purification