title: Effect of confinement and kinetics on the morphology of phase separating gelatin-maltodextrin droplets.
abstract: The effect of confinement on the structure evolution and final morphology during phase separation and gelation of gelatin and maltodextrin was investigated and compared to the structures seen in bulk phase. Emulsion droplets with diameters from 4 to 300 mum were analyzed using confocal laser scanning microscopy and image analysis. With the confocal laser scanning microscope it was possible to follow the entire phase separating process inside the droplets in real-time. The samples were either quenched directly from 70 degrees C down to 20 degrees C or exposed to holding times at 40 degrees C. Different cooling procedures were studied to examine the structure evolution both before and after gelation in the restricted geometries. The concentration of the biopolymer mixture was kept constant at 4 w/w% gelatin and 6 w/w% maltodextrin. The results revealed that the size of the confinement had a great effect on both the initiation of phase separation and the final morphology of the microstructure inside the emulsion droplets. The phase separation in small droplets was observed to occur at a temperature above the phase separating temperature for bulk. Small droplets had either a microstructure with a shell of maltodextrin and core of gelatin or a microstructure where the two biopolymers had formed two separate bicontinuous halves. The initiation of phase separation in large droplets was similar to what was seen in bulk. The microstructure in large droplets was discontinuous, resembling the morphology in bulk phase. The kinetics had an effect on the character of the maltodextrin inclusions, as the cooling procedure of a direct quench gave spherical inclusions with an even size distribution, while a holding time at 40 degrees C resulted in asymmetrical and elongated inclusions.
authors.affiliation: SIK, The Swedish Institute for Food and Biotechnology, Gothenburg, Sweden.
authors.forename: Sophia
authors.initials: S
authors.lastname: Fransson
authors.forename: Niklas
authors.initials: N
authors.lastname: Lorén
authors.forename: Annika
authors.initials: A
authors.lastname: Altskär
authors.forename: Anne-Marie
authors.initials: AM
authors.lastname: Hermansson
mesh_terms: D005780:Gelatin / Q000737:chemistry*; D007700:Kinetics; D018613:Microscopy, Confocal; D011134:Polysaccharides / Q000737:chemistry*
chemical_list: D011134:Polysaccharides; C008315:maltodextrin; D005780:Gelatin