title: Dry olive leaf extract ameliorates experimental autoimmune encephalomyelitis.
abstract: OBJECTIVE
  Experimental autoimmune encephalomyelitis (EAE) is an animal model of CNS inflammatory and demyelinating disease multiple sclerosis. Mediterranean diet, rich in olive products is associated with lower incidence of multiple sclerosis in South European population. Therefore, the influence of dry olive leaf extract (DOLE) on EAE course was investigated.
  
  
  METHODS
  Spinal cord homogenate and complete Freund's adjuvant were used for the induction of EAE in Dark Agouti rats. DOLE was applied intragastrically once per day, starting from the day of the immunization. Real time PCR and ELISA were used for the determination of IFN-gamma and IL-17 gene expression and production, respectively.
  
  
  RESULTS
  DOLE reduced various parameters of EAE severity in DA rats, including cumulative disease index, maximal clinical score and disease duration. Also, DOLE decreased cellularity of the draining lymph nodes and production of IFN-gamma and IL-17 by the cells infiltrating spinal cord of EAE rats.
  
  
  CONCLUSIONS
  The results presented in this paper strongly suggest that DOLE-enriched diet has a beneficial effect in EAE in rats. Further studies in humans are required in order to investigate if DOLE could be a useful supplementary dietetic for the patients suffering from multiple sclerosis and other neuroinflammatory disorders.
authors.affiliation: Institute for Biological Research Sinisa Stanković, Department of Immunology, University of Belgrade, Belgrade, Serbia. georgije_zw@yahoo.com
authors.forename: Djordje
authors.initials: D
authors.lastname: Miljković
authors.forename: Dragana
authors.initials: D
authors.lastname: Dekanski
authors.forename: Zeljka
authors.initials: Z
authors.lastname: Miljković
authors.forename: Miljana
authors.initials: M
authors.lastname: Momcilović
authors.forename: Marija
authors.initials: M
authors.lastname: Mostarica-Stojkovic
mesh_terms: D000818:Animals; D038441:Diet, Mediterranean; D004198:Disease Susceptibility; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000188:drug therapy* / Q000473:pathology; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005620:Freund's Adjuvant / Q000633:toxicity; D007371:Interferon-gamma / Q000235:genetics / Q000378:metabolism; D020381:Interleukin-17 / Q000235:genetics / Q000378:metabolism; D008297:Male; D018696:Neuroprotective Agents / Q000494:pharmacology; D031658:Olea / Q000737:chemistry*; D010936:Plant Extracts / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry; D051381:Rats; D011919:Rats, Inbred Strains; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012720:Severity of Illness Index
chemical_list: D020381:Interleukin-17; D018696:Neuroprotective Agents; D010936:Plant Extracts; D007371:Interferon-gamma; D005620:Freund's Adjuvant