Serologic evaluation of clinical and subclinical secondary hepatic amyloidosis in rhesus macaques (Macaca mulatta).
Secondary hepatic amyloidosis in nonhuman primates carries a grave prognosis once animals become clinically ill. The purpose of this study was to establish serologic parameters that potentially could be used to identify rhesus macaques undergoing subclinical development of secondary hepatic amyloidosis. A retrospective analysis was completed by using serum biochemical profiles from 26 histologically diagnosed amyloidotic macaques evaluated at 2 stages of disease, clinical and subclinical (3 to 32 mo prior to clinical signs of disease). Standard serum biochemistry values for cases were compared with institutional age- and gender-specific references ranges by construction of 95% confidence intervals for the difference between means. In addition, 19 histologically diagnosed amyloidotic macaques and 19 age-matched controls were assayed for changes in various parameters by using routinely banked, frozen (-80 degrees C) sera available from clinical and subclinical time points. Clinically amyloidotic animals displayed increased levels of alkaline phosphatase, aspartate aminotransferase, lactate dehydrogenase, gamma glutamyltranspeptidase, and macrophage colony-stimulating factor and significantly decreased quantities of albumin and total cholesterol. Subclinical amyloidotic animals displayed increased levels of alkaline phosphatase, aspartate aminotransferase, lactate dehydrogenase, and serum amyloid A and decreased concentrations of albumin and total cholesterol. The serologic parameters studied indicate a temporal relationship of these factors not previously described, show a clear pattern of disease progression, and could be useful in subclinical disease detection.
MacGuire
Jamus G
JG
California National Primate Research Center, University of California, Davis, California, USA. jmacguire@primate.ucdavis.edu
Christe
Kari L
KL
Yee
JoAnn L
JL
Kalman-Bowlus
Alexis L
AL
Lerche
Nicholas W
NW
D000686:Amyloidosis / Q000097:blood* / Q000175:diagnosis / Q000473:pathology / Q000662:veterinary*; D000818:Animals; D001774:Blood Chemical Analysis; D018450:Disease Progression; D005260:Female; D006801:Humans; D007118:Immunoassay; D008099:Liver / Q000201:enzymology; D008107:Liver Diseases / Q000097:blood* / Q000175:diagnosis / Q000473:pathology / Q000662:veterinary*; D008253:Macaca mulatta; D016173:Macrophage Colony-Stimulating Factor / Q000097:blood; D008297:Male; D008992:Monkey Diseases / Q000097:blood* / Q000175:diagnosis / Q000473:pathology; D012189:Retrospective Studies; D012698:Serologic Tests
D016173:Macrophage Colony-Stimulating Factor