Protein identification and mRNA analysis of phytochrome-regulated genes in Arabidopsis under red light.
Phytochromes are a family of plant photoreceptors that mediate physiological and developmental responses to red and far-red light. According to the affymetrix ATH1 microarray, phytochrome A (phyA) and phytochrome B (phyB) together play a key role in transducing the Rc signals to light-responsive genes. In order to select those red light-responsive genes associated with phyA or phyB, a proteomic approach based on two-dimensional gel electrophoresis (2-DE) was used to compare the protein expression patterns of the phyAphyB double mutant and the wild type of Arabidopsis thaliana (col-4) which grew under constant red light conditions for 7 d. Thirty-two protein spots which exhibited differences in protein abundance were identified by matrix-assisted laser desorption/ionization-time of flight/time of flight mass spectrometry. The expression of ten genes corresponding to ten protein spots was analyzed by a semiquantitative reverse transcription-polymerase chain reaction. Two of the ten genes were confirmed by quantitative PCR (Q-PCR). The results showed that phytochromes may exert their function by regulating mRNA or protein expressions. Proteomic analysis may provide a novel pathway for identifying phytochrome-dependent genes.
Li
Xu
X
Bioenergy and Biomaterial Research Center, College of Life Science and Biotechnology, Hunan University, Changsha 410082, China.
Yang
YueJun
Y
Li
Yan
Y
Wang
Jie
J
Xiao
XiaoJuan
X
Guo
XinHong
X
Tang
DongYing
D
Liu
XuanMing
X
D017360:Arabidopsis / Q000235:genetics* / Q000378:metabolism / Q000528:radiation effects; D001483:Base Sequence; D017931:DNA Primers; D015180:Electrophoresis, Gel, Two-Dimensional; D010834:Phytochrome / Q000502:physiology*; D010940:Plant Proteins / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000378:metabolism*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D017931:DNA Primers; D010940:Plant Proteins; D012333:RNA, Messenger; D010834:Phytochrome