title: Cytochrome oxidase from Pseudomonas aeruginosa. III. Reduction of hydroxylamine.
abstract: Pseudomonas aeruginosa cytochrome oxidase, which reduces nitrite and oxygen, is also capable of reducing hydroxylamine to ammonia. The Km for hydroxylamine reduction is 6 x 10(-4) M compared to 5 x 10(-5) M for nitrite reduction. NADH, NADPH, reduced P. aeruginosa cytochrome c551, and reduced P. aeruginosa copper protein were ineffective as electron donors for hydroxylamine reduction whereas reduced pyocyanine and methylene blue acted as electron mediators.
authors.affiliation: Section of Biochemistry, Molecular and Cell Biology, Cornell University, Ithaca, NY 14850, USA.
authors.forename: J
authors.initials: J
authors.lastname: Singh
mesh_terms: D001426:Bacterial Proteins / Q000378:metabolism*; D003576:Electron Transport Complex IV / Q000378:metabolism*; D006418:Heme / Q000031:analogs & derivatives / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D019811:Hydroxylamine / Q000378:metabolism*; D010084:Oxidation-Reduction; D010088:Oxidoreductases / Q000037:antagonists & inhibitors / Q000378:metabolism; D011550:Pseudomonas aeruginosa / Q000201:enzymology*
chemical_list: D001426:Bacterial Proteins; D019811:Hydroxylamine; D006418:Heme; C080647:heme d; D010088:Oxidoreductases; C021402:hydroxylamine reductase; D003576:Electron Transport Complex IV