title: Evaluation of five commercial real-time PCR assays for detection of Mycoplasma pneumoniae in respiratory tract specimens.
abstract: The performances of five commercial TaqMan real-time PCR assays for the detection of Mycoplasma pneumoniae in respiratory tract specimens were evaluated in comparison with an in-house real-time PCR. All kits allowed prompt and specific results, validated by the use of an internal control. The Nanogen kit showed the best clinical sensitivity.
authors.affiliation: Service des Laboratoires, Centre National de Greffe de Moelle Osseuse, Tunis, Tunisia.
authors.forename: A
authors.initials: A
authors.lastname: Touati
authors.forename: A
authors.initials: A
authors.lastname: Benard
authors.forename: A Ben
authors.initials: AB
authors.lastname: Hassen
authors.forename: C M
authors.initials: CM
authors.lastname: Bébéar
authors.forename: S
authors.initials: S
authors.lastname: Pereyre
mesh_terms: D006801:Humans; D025202:Molecular Diagnostic Techniques / Q000379:methods; D009177:Mycoplasma pneumoniae / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000379:methods*; D011933:Reagent Kits, Diagnostic; D012137:Respiratory System / Q000382:microbiology*; D012680:Sensitivity and Specificity
chemical_list: D011933:Reagent Kits, Diagnostic