title: Evaluation of a commercial competitive ELISA for the detection of antibodies to Rift Valley fever virus in sera of domestic ruminants in France.
abstract: This paper describes the sensitivity and specificity of a commercial competitive ELISA for the detection of antibodies to Rift Valley fever virus (RVFV) from sera of French domestic ruminants. Field samples were collected from mainland France for the known-negative sera (cattle=191, goats=119, sheep=192) and from ruminants of a French overseas territory (Mayotte) for the known-positive sera. A cut-off value of 43% was determined for all species, achieving a sensitivity and specificity of 100% and a concordance of 100% with the species-specific threshold recommended by the manufacturer. Our results demonstrate that this ELISA may be a suitable diagnostic tool for disease surveillance programmes and import/export veterinary certification of French cattle, goats and sheep.
authors.affiliation: CIRAD, UMR Contrôle des Maladies, Montpellier F-34398, France. catherine.cetre-sossah@cirad.fr
authors.forename: Catherine
authors.initials: C
authors.lastname: Cêtre-Sossah
authors.forename: Agnès
authors.initials: A
authors.lastname: Billecocq
authors.forename: Renaud
authors.initials: R
authors.lastname: Lancelot
authors.forename: Cédric
authors.initials: C
authors.lastname: Defernez
authors.forename: Jacques
authors.initials: J
authors.lastname: Favre
authors.forename: Michèle
authors.initials: M
authors.lastname: Bouloy
authors.forename: Dominique
authors.initials: D
authors.lastname: Martinez
authors.forename: Emmanuel
authors.initials: E
authors.lastname: Albina
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D002417:Cattle; D002418:Cattle Diseases / Q000175:diagnosis / Q000453:epidemiology; D004797:Enzyme-Linked Immunosorbent Assay / Q000592:standards / Q000662:veterinary*; D005260:Female; D005602:France / Q000453:epidemiology; D015511:Goat Diseases / Q000175:diagnosis / Q000453:epidemiology; D006041:Goats; D008297:Male; D011159:Population Surveillance; D012295:Rift Valley Fever / Q000175:diagnosis / Q000453:epidemiology / Q000662:veterinary*; D012296:Rift Valley fever virus / Q000276:immunology*; D012418:Ruminants / Q000821:virology*; D012680:Sensitivity and Specificity; D012756:Sheep; D012757:Sheep Diseases / Q000175:diagnosis / Q000453:epidemiology; D013045:Species Specificity
chemical_list: D000914:Antibodies, Viral