[Bone marrow stem cell mobilization to the peripheral blood and tumor necrosis factor-alpha expression in ischemic myocardium in rabbit models of hibernating myocardium].
OBJECTIVE
To investigate the changes in peripheral blood bone marrow stem cells and tumor necrosis factor-alpha gene expression in the ischemic myocardium in rabbit models of hibernating myocardium.


METHODS
Twenty-four male Japanese white rabbits were randomized into 4 groups, including a sham-operated group and 3 model groups with hibernating myocardium induced by partial ligation of the left anterior descending coronary artery. The percentage of CD34-positive cells in the peripheral blood was evaluated by flow cytometry, and TNF-alpha mRNA expression in the ischemic myocardium was determined by real-time RT-PCR in the 3 model groups (at 3, 7, or 28 days after the operation) and in the sham-operated group.


RESULTS
In rabbits with partial ligation of the left anterior descending coronary artery, the percentage of CD34-positive cells in the peripheral blood and myocardial TNF-alpha mRNA expression were significantly increased at 3 and 7 days after the operation in comparison with those in the sham-operated group and those at 28 days postoperatively (P<0.01). No significant differences were found in the percentage of CD34 positive cells or myocardial TNF-alpha mRNA expression between the sham-operated group and the rabbits 28 days after the coronary artery ligation (P>0.05).


CONCLUSIONS
Bone marrow stem cell can be mobilized into the peripheral blood in rabbit hibernating myocardium model possibly by increasing TNF-alpha gene expression in the ischemic myocardium.
Zhao
Qing-bin
QB
Department of Gerontology, First Affiliated Hospital of Xi'an Jiaotong University College of Medicine, Xi'an 710061, China. zhaoqingbin05 @163.com
Sun
Chao-feng
CF
Ma
Ai-qun
AQ
Cui
Chang-cong
CC
Wu
Yue
Y
Tian
Yu-ling
YL
Zhao
Yan
Y
D000818:Animals; D018952:Antigens, CD34 / Q000378:metabolism; D001854:Bone Marrow Cells / Q000166:cytology*; D003331:Coronary Vessels / Q000601:surgery; D004195:Disease Models, Animal; D005786:Gene Expression Regulation; D019650:Hematopoietic Stem Cell Mobilization; D006605:Hibernation; D008026:Ligation; D008297:Male; D017202:Myocardial Ischemia / Q000378:metabolism* / Q000503:physiopathology / Q000601:surgery / Q000628:therapy*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011817:Rabbits; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics*
D018952:Antigens, CD34; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha