Ectopic expression of a rice protein phosphatase 2C gene OsBIPP2C2 in tobacco improves disease resistance.
Protein phosphatase 2Cs (PP2Cs) have been demonstrated to play critical roles in regulation of plant growth/development, abscisic acid signaling pathway and adaptation to environmental stresses. Here we report the cloning and molecular characterization of a novel rice protein phosphatase 2C gene, OsBIPP2C2 (Oryza sativa L. BTH-induced protein phosphatase 2C 2). OsBIPP2C2 has three alternatively spliced transcripts and the largest transcript OsBIPP2C2a encodes a 380 aa protein containing all 11 conserved catalytic subdomains of PP2Cs. Expression of OsBIPP2C2a was significantly induced by benzothiadiazole (BTH), one of defense-related signal molecules in plants. Expression of OsBIP2C2a was induced by infection with the blast fungus, Magnaporthe grisea, and the pathogen-induced expression of OsBIPP2C2a in BTH-treated rice seedlings was much earlier and stronger than those in water-treated seedlings. Overexpression of OsBIPP2C2a in transgenic tobacco plants resulted in increased disease resistance against tobacco mosaic virus and Phytophthora parasitica var. nicotianae. Importantly, the OsBIPP2C2a-overexpressing transgenic tobacco plants showed constitutive expression of defense-related genes. These results suggest that OsBIPP2C2a may play an important role in disease resistance through activation of defense response.
Hu
Xuebo
X
State Key Laboratory of Rice Biology, Department of Plant Protection, Institute of Biotechnology, Zhejiang University-Huajiachi Campus, 310029, Hangzhou, Zhejiang, People's Republic of China.
Zhang
Huijuan
H
Li
Guojun
G
Yang
Yuxia
Y
Zheng
Zhong
Z
Song
Fengming
F
D017398:Alternative Splicing; D000595:Amino Acid Sequence; D003001:Cloning, Molecular; D018744:DNA, Plant / Q000235:genetics; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D007113:Immunity, Innate; D020082:Magnaporthe / Q000502:physiology; D008969:Molecular Sequence Data; D012275:Oryza / Q000235:genetics*; D010749:Phosphoprotein Phosphatases / Q000235:genetics / Q000378:metabolism*; D010935:Plant Diseases / Q000235:genetics; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D030821:Plants, Genetically Modified / Q000235:genetics / Q000276:immunology; D000071636:Protein Phosphatase 2C; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D013830:Thiadiazoles / Q000494:pharmacology; D014026:Nicotiana / Q000235:genetics* / Q000276:immunology
D018744:DNA, Plant; D010940:Plant Proteins; D013830:Thiadiazoles; C015700:benzo-1,2,3-thiadiazole; D010749:Phosphoprotein Phosphatases; D000071636:Protein Phosphatase 2C