title: Determination of matrix metalloproteinases in human radicular dentin.
abstract: Matrix metalloproteinases (MMPs) are present in sound coronal dentin and may play a role in collagen network degradation in bonded restorations. We investigated whether these enzymes can also be detected in root dentin. Crown and root sections of human teeth were powderized, and dentin proteins were extracted by using guanidine-HCl and EDTA. Extracts were analyzed by zymography and Western blotting for matrix metalloproteinases detection. Zymography revealed gelatinolytic activities in both crown and root dentin samples, corresponding to MMP-2 and MMP-9. MMP-2 was more evident in demineralized root dentin matrix, whereas MMP-9 was mostly extracted from the mineralized compartment of dentin and presented overall lower levels. Western blot analysis detected MMP-8 equally distributed in crown and root dentin. Because MMPs are also present in radicular dentin, their contribution to the degradation of resin-dentin bonds should be addressed in the development of restorative strategies for the root substrate.
authors.affiliation: Department of Restorative Dentistry, Endodontics Area, Piracicaba Dental School, University of Campinas, Piracicaba, SP, Brazil.
authors.forename: Juliana
authors.initials: J
authors.lastname: Santos
authors.forename: Marcela
authors.initials: M
authors.lastname: Carrilho
authors.forename: Taina
authors.initials: T
authors.lastname: Tervahartiala
authors.forename: Timo
authors.initials: T
authors.lastname: Sorsa
authors.forename: Lorenzo
authors.initials: L
authors.lastname: Breschi
authors.forename: Annalisa
authors.initials: A
authors.lastname: Mazzoni
authors.forename: David
authors.initials: D
authors.lastname: Pashley
authors.forename: Franklin
authors.initials: F
authors.lastname: Tay
authors.forename: Caio
authors.initials: C
authors.lastname: Ferraz
authors.forename: Leo
authors.initials: L
authors.lastname: Tjäderhane
mesh_terms: D000328:Adult; D015153:Blotting, Western; D004396:Coloring Agents; D003804:Dentin / Q000201:enzymology*; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D020778:Matrix Metalloproteinase 2 / Q000032:analysis; D020784:Matrix Metalloproteinase 8 / Q000032:analysis; D020780:Matrix Metalloproteinase 9 / Q000032:analysis; D020782:Matrix Metalloproteinases / Q000032:analysis*; D008970:Molecular Weight; D019228:Tooth Crown / Q000201:enzymology; D017001:Tooth Demineralization / Q000201:enzymology; D014092:Tooth Root / Q000201:enzymology*; D055815:Young Adult
chemical_list: D004396:Coloring Agents; D020782:Matrix Metalloproteinases; C522361:MMP2 protein, human; D020778:Matrix Metalloproteinase 2; C488762:MMP8 protein, human; D020784:Matrix Metalloproteinase 8; D020780:Matrix Metalloproteinase 9