[Studies on application of ultra-filtration to purifying polysaccharides from Rhubarb].
OBJECTIVE
To purify Rhubarb polysaccharides via micro-filtration and ultra-filtration.


METHODS
The technology adopted micro-filtration and tubular membrane with different cut-off molecular weights.


RESULTS
Experiment results showed that the optimum operating conditions were that the ultra-filtration time controlled around 80 min, the temperature was set the 35-40 degrees C, the operating pressure was 0.08-0.12 MPa, the pH was 6-8, and a sample that of polysaccharide was 0.5 times of original concentration. The recovery ratio of the polysaccharides was 53.7%, the concentration could be condensed to 2.73 times compared with the sample liquid.


CONCLUSIONS
The technology was simple and feasible, it can be applied to purify Rhubarb polysaccharides.
Sun
Yu
Y
Modern Engineering Center for Chinese Traditional Medicine, East China University of Science and Technology, Shanghai 200237, China.
Zhou
Yongchuan
Y
Chen
Dexu
D
D005240:Feasibility Studies; D006863:Hydrogen-Ion Concentration; D011134:Polysaccharides / Q000302:isolation & purification*; D011312:Pressure; D012250:Rheum / Q000737:chemistry*; D013696:Temperature; D013997:Time Factors; D014462:Ultrafiltration / Q000379:methods*
D011134:Polysaccharides