Apolipoprotein A1 gene polymorphisms as risk factors for hypertension and obesity.
Several polymorphisms in apolipoprotein A1 (APOA1) gene have been associated with metabolic diseases. Increased transcription efficiency was observed in -75A allele carriers compared to -75G allele homozygotes. +83C allele was associated with higher body mass index and waist-to-hip ratio in type II diabetes subjects. -75G/A and +83C/T polymorphisms were analyzed by RFLP-PCR in 334 individuals from a Brazilian elderly cohort. APOA1 polymorphisms were associated with age-related morbidities, as well as with triglycerides, total cholesterol, HDL, VLDL, LDL, creatinine, urea, albumin, glycated hemoglobin and fasting glucose serum levels. Allele frequencies were 0.102 and 0.21, respectively, for -75A and +83T. -75G allele showed significant association with hypertension (P = 0.001). An association between +83C allele and obesity was observed (P = 0.040) and this allele also showed an association with hypertension in the presence of cardiovascular disease (P = 0.047). Moreover, +83T allele was associated with lower glycated hemoglobin values (P = 0.026). To our knowledge, there is no data associating this polymorphism with glycated hemoglobin. Furthermore, individuals carrying AT haplotype have lower risk for developing hypertension (P = 0.0002), while GT haplotype carriers present decreased risk to develop obesity comparing to GC haplotype (P = 0.025). APOA1 polymorphisms analysis may be a useful tool to identify risk factors for subjects and families and clarify the physiopathological role of these polymorphisms in age-related diseases, such as hypertension and obesity.
Chen
Elizabeth Suchi
ES
Disciplina de Genética, Departamento de Morfologia e Genética, Escola Paulista de Medicina/Universidade Federal de São Paulo, Rua Botucatu, 740, Edifício Leitão da Cunha-1 andar, São Paulo, SP, CEP 04023-900, Brazil.
Mazzotti
Diego Robles
DR
Furuya
Tatiane Katsue
TK
Cendoroglo
Maysa Seabra
MS
Ramos
Luiz Roberto
LR
Araujo
Lara Quirino
LQ
Burbano
Rommel Rodriguez
RR
de Arruda Cardoso Smith
Marília
M
D000368:Aged; D000369:Aged, 80 and over; D000483:Alleles; D016632:Apolipoprotein A-I / Q000097:blood / Q000235:genetics*; D015992:Body Mass Index; D001938:Brazil; D002318:Cardiovascular Diseases / Q000097:blood / Q000150:complications / Q000235:genetics; D002784:Cholesterol / Q000097:blood; D015331:Cohort Studies; D005260:Female; D005787:Gene Frequency; D006239:Haplotypes / Q000235:genetics; D006801:Humans; D006973:Hypertension / Q000097:blood / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D009765:Obesity / Q000097:blood / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic / Q000235:genetics*; D012150:Polymorphism, Restriction Fragment Length; D012307:Risk Factors; D014280:Triglycerides / Q000097:blood
D016632:Apolipoprotein A-I; D014280:Triglycerides; D002784:Cholesterol