[Effect of angiotensin1-7 on alpha-smooth muscle actin protein expression in rat hepatic stellate cells].
OBJECTIVE
To investigate the effect of angiotensin II and angiotensin1-7 on alpha-smooth muscle actin (alpha-SMA)-induced Ca(2+)-independent pathways mediated by Rho kinase2 in hepatic stellate cells (HSCs).


METHODS
HSC-T6 cells were treated with 10 micromol/L of AngII, Ang1-7, AngII +Ang1-7, and Ang1-7+A779. RT-PCR was used to detect the expression of Rho kinase2 (Rock2) in Ca(2+)-independent pathways, and alpha-SMA protein expression was detected by Western blotting.


RESULTS
The mRNA expression of Rock2 increased significantly in the cells after AngII treatment (P<0.01), but decreased following Ang1-7 treatment. Ang1-7 treatment significantly reduced alpha-SMA level in AngII-induced cells (P<0.01).


CONCLUSIONS
Ang1-7 can inhibit AngII-induced activation of Rock2 and reduce alpha-SMA expression in HSCs.
Ying
Song-song
SS
Department of Digestive Diseases, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
Li
Xu
X
Huang
Mao-liang
ML
Meng
Ying
Y
Zhang
Zhen-shu
ZS
D000199:Actins / Q000378:metabolism*; D000803:Angiotensin I / Q000494:pharmacology*; D000804:Angiotensin II / Q000494:pharmacology; D000818:Animals; D002118:Calcium / Q000378:metabolism; D002460:Cell Line; D005786:Gene Expression Regulation / Q000187:drug effects*; D055166:Hepatic Stellate Cells / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism*; D010446:Peptide Fragments / Q000494:pharmacology*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D015398:Signal Transduction / Q000187:drug effects; D054460:rho-Associated Kinases / Q000235:genetics
D000199:Actins; D000803:Angiotensin I; D000804:Angiotensin II; D002118:Calcium; D010446:Peptide Fragments; D012333:RNA, Messenger; D054460:rho-Associated Kinases; C498862:Acta2 protein, rat; C118790:angiotensin I (1-7)