title: Two different homing pathways involving integrin β7 and E-selectin significantly influence trafficking of CD4 cells to the genital tract following Chlamydia muridarum infection.
abstract: OBJECTIVE
  Chlamydia trachomatis causes STI and reproductive dysfunction worldwide which is not preventable with antibiotics. Identifying a population of endocervical T cells to target in vaccine development would enhance efficacy.
  
  
  METHODS
  Trafficking of murine CD4+ lymphocytes to Chlamydia muridarum infected genital tract (GT) tissue in vivo was measured using adoptive transfer studies of fluorescent CD4+ T cells from integrin β7-/- mice or mice which lack E-selectin on endothelial cells.
  
  
  RESULTS
  Murine in vivo migration studies showed that lack of α4β7 or E-selectin significantly reduced trafficking of CD4 T cells to the GT of mice infected with C. muridarum.
  
  
  CONCLUSIONS
  CD4+ T cells use at least two different adhesive mechanisms involving an integrin of the mucosal homing pathway and selectin pathway to accumulate in the GT during C. muridarum infection.
authors.affiliation: Department of Pathology & Laboratory Medicine, Geffen School of Medicine, University of California, Los Angeles, CA 90095-1732, USA. kkelly@mednet.ucla.edu
authors.forename: Kathleen A
authors.initials: KA
authors.lastname: Kelly
authors.forename: Ann M
authors.initials: AM
authors.lastname: Chan
authors.forename: Anthony
authors.initials: A
authors.lastname: Butch
authors.forename: Toni
authors.initials: T
authors.lastname: Darville
mesh_terms: D000818:Animals; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D002465:Cell Movement / Q000235:genetics / Q000276:immunology*; D002690:Chlamydia Infections / Q000235:genetics / Q000276:immunology* / Q000382:microbiology; D023502:Chlamydia muridarum / Q000276:immunology*; D002692:Chlamydia trachomatis; D019040:E-Selectin / Q000235:genetics / Q000276:immunology*; D005260:Female; D005836:Genitalia, Female / Q000276:immunology* / Q000382:microbiology; D039641:Integrin beta Chains / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D018345:Mice, Knockout
chemical_list: D019040:E-Selectin; D039641:Integrin beta Chains; C091368:integrin beta7