title: Antibacterial discovery in actinomycetes strains with mutations in RNA polymerase or ribosomal protein S12.
abstract: We show that selection of drug-resistant bacterial mutants allows the discovery of antibacterial compounds. Mutant strains of a soil-isolated Streptomyces species that does not produce antibacterials synthesize a previously unknown class of antibacterial, which we name piperidamycin. Overall, 6% of non-Streptomyces actinomycetes species and 43% of Streptomyces species that do not produce antibacterials are activated to produce them. The antibacterial-producing mutants all carried mutations in RNA polymerase and/or the ribosomal protein S12.
authors.affiliation: National Food Research Institute, Tsukuba, Ibaraki, Japan.
authors.forename: Takeshi
authors.initials: T
authors.lastname: Hosaka
authors.forename: Mayumi
authors.initials: M
authors.lastname: Ohnishi-Kameyama
authors.forename: Hideyuki
authors.initials: H
authors.lastname: Muramatsu
authors.forename: Kana
authors.initials: K
authors.lastname: Murakami
authors.forename: Yasuhisa
authors.initials: Y
authors.lastname: Tsurumi
authors.forename: Shinya
authors.initials: S
authors.lastname: Kodani
authors.forename: Mitsuru
authors.initials: M
authors.lastname: Yoshida
authors.forename: Akihiko
authors.initials: A
authors.lastname: Fujie
authors.forename: Kozo
authors.initials: K
authors.lastname: Ochi
mesh_terms: D039903:Actinobacteria / Q000235:genetics / Q000378:metabolism; D000900:Anti-Bacterial Agents / Q000032:analysis*; D012321:DNA-Directed RNA Polymerases / Q000235:genetics*; D055808:Drug Discovery; D009154:Mutation; D012269:Ribosomal Proteins / Q000235:genetics*; D013302:Streptomyces / Q000737:chemistry* / Q000235:genetics* / Q000378:metabolism
chemical_list: D000900:Anti-Bacterial Agents; D012269:Ribosomal Proteins; C034212:ribosomal protein S12; D012321:DNA-Directed RNA Polymerases