Thermostabilization mechanism of bovine serum albumin by trehalose.
Thermal denaturation of bovine serum albumin (BSA) is analyzed from differential scanning calorimetry (DSC) and Raman spectroscopy investigations. DSC curves exhibit a marked dependence on protein concentration. BSA thermal denaturation becomes broader and bimodal, and the temperature of denaturation increases with increasing protein concentration. Raman scattering investigations simultaneously carried out in the low-frequency range (10-350 cm(-1)) and in the amide I band region (1500-1800 cm(-1)) indicate that the denaturation process is described as a biphasic process independent of protein concentration. The dependence of the protein stability upon the protein concentration can be interpreted from the coupling of protein and solvent dynamics. The confrontation of previous results obtained from Raman investigations on lysozyme (LYS) and the present study of BSA brings out significant information on protein dynamics and the coupling of protein and hydration-water dynamics in relation with the solvent accessible surface area. Contrary to LYS, the modification of the dynamics of hydration water by the protein is clearly observed on BSA. The influence of trehalose on the protein dynamics was analyzed. We found that trehalose reduces the dynamic fluctuations of polar side chains at the protein-solvent interface. The mechanism of thermostabilization by trehalose is related to the reduction of the exposure of hydrophobic groups of BSA to the water molecules, and to a strengthening of intermolecular O-H interactions in the hydrogen-bond network of water, leading to the stabilization of the tertiary structure.
Hédoux
Alain
A
Laboratoire de Dynamique et Structure des Materiaux Moleculaires, UMR CNRS 8024, UFR de Physique, Universite de Lille 1, Bat. P5, 59 655 Villeneuve d'Ascq Cedex, France.
Willart
Jean-François
JF
Paccou
Laurent
L
Guinet
Yannick
Y
Affouard
Frédéric
F
Lerbret
Adrien
A
Descamps
Marc
M
D000577:Amides / Q000737:chemistry; D002152:Calorimetry, Differential Scanning; D006860:Hydrogen Bonding; D011489:Protein Denaturation; D055550:Protein Stability; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D013059:Spectrum Analysis, Raman; D013696:Temperature; D013816:Thermodynamics; D014199:Trehalose / Q000737:chemistry*; D014867:Water / Q000737:chemistry
D000577:Amides; D014867:Water; D012710:Serum Albumin, Bovine; D014199:Trehalose