title: Benzaldehyde dehydrogenase from chitosan-treated Sorbus aucuparia cell cultures.
abstract: Cell cultures of Sorbus aucuparia respond to the addition of chitosan with the accumulation of the biphenyl phytoalexin aucuparin. The carbon skeleton of this inducible defense compound is formed by biphenyl synthase (BIS) from benzoyl-CoA and three molecules of malonyl-CoA. The formation of benzoyl-CoA proceeds via benzaldehyde as an intermediate. Benzaldehyde dehydrogenase (BD), which converts benzaldehyde into benzoic acid, was detected in cell-free extracts from S. aucuparia cell cultures. BD and BIS were induced by chitosan treatment. The preferred substrate for BD was benzaldehyde (K(m)=49 microM). Cinnamaldehyde and various hydroxybenzaldehydes were relatively poor substrates. BD activity was strictly dependent on the presence of NAD(+) as a cofactor (K(m)=67 microM).
authors.affiliation: Institute of Pharmaceutical Biology, Technical University of Braunschweig, Mendelssohnstrasse 1, D-38106 Braunschweig, Germany.
authors.forename: Mariam M
authors.initials: MM
authors.lastname: Gaid
authors.forename: Debabrata
authors.initials: D
authors.lastname: Sircar
authors.forename: Till
authors.initials: T
authors.lastname: Beuerle
authors.forename: Adinpunya
authors.initials: A
authors.lastname: Mitra
authors.forename: Ludger
authors.initials: L
authors.lastname: Beerhues
mesh_terms: D000445:Aldehyde Oxidoreductases / Q000378:metabolism*; D001547:Benzaldehydes / Q000737:chemistry; D055162:Biocatalysis / Q000187:drug effects; D002478:Cells, Cultured; D048271:Chitosan / Q000494:pharmacology*; D002851:Chromatography, High Pressure Liquid; D008401:Gas Chromatography-Mass Spectrometry; D007700:Kinetics; D009097:Multienzyme Complexes / Q000378:metabolism; D031994:Sorbus / Q000166:cytology / Q000187:drug effects* / Q000201:enzymology*; D013379:Substrate Specificity / Q000187:drug effects
chemical_list: D001547:Benzaldehydes; D009097:Multienzyme Complexes; C482658:biphenyl synthase, Sorbus aucuparia; D048271:Chitosan; D000445:Aldehyde Oxidoreductases; C027015:benzaldehyde dehydrogenase (NAD+); C032175:benzaldehyde