title: Steroids from the leaves of Chinese Melia azedarach and their cytotoxic effects on human cancer cell lines.
abstract: Three new (1-3) and several known (4-6) steroids were isolated from the leaves of Chinese Melia azedarach. The structures of the new compounds were elucidated by means of spectroscopic methods including 2D NMR techniques and mass spectrometry to be (20S)-5,24(28)-ergostadiene-3beta,7alpha,16beta,20-tetrol (1), (20S)-5-ergostene-3beta,7alpha,16beta,20-tetrol (2), and 2alpha,3beta-dihydro-5-pregnen-16-one (3). The cytotoxicities of the isolated compounds against three human cancer cell lines (A549, H460, U251) were evaluated; only compounds 1, 2, and (20S)-5-stigmastene-3beta,7alpha,20-triol (4) were found to show significant cyctotoxic effects with IC(50)s from 12.0 to 30.1 microg/mL.
authors.affiliation: Department of Natural Products for Chemical Genetic Research, Key Laboratory of Brain Functional Genomics, Ministry of Education, East China Normal University, Shanghai, PR China.
authors.forename: Shi-Biao
authors.initials: SB
authors.lastname: Wu
authors.forename: Yan-Ping
authors.initials: YP
authors.lastname: Ji
authors.forename: Jing-Jing
authors.initials: JJ
authors.lastname: Zhu
authors.forename: Yun
authors.initials: Y
authors.lastname: Zhao
authors.forename: Gang
authors.initials: G
authors.lastname: Xia
authors.forename: Ying-He
authors.initials: YH
authors.lastname: Hu
authors.forename: Jin-Feng
authors.initials: JF
authors.lastname: Hu
mesh_terms: D000970:Antineoplastic Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology* / Q000633:toxicity; D045744:Cell Line, Tumor; D002681:China; D006801:Humans; D020128:Inhibitory Concentration 50; D009682:Magnetic Resonance Spectroscopy; D031586:Melia azedarach / Q000737:chemistry*; D018515:Plant Leaves / Q000737:chemistry; D013256:Steroids / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology* / Q000633:toxicity
chemical_list: D000970:Antineoplastic Agents; D013256:Steroids