Evaluation of five commercial real-time PCR assays for detection of Mycoplasma pneumoniae in respiratory tract specimens.
The performances of five commercial TaqMan real-time PCR assays for the detection of Mycoplasma pneumoniae in respiratory tract specimens were evaluated in comparison with an in-house real-time PCR. All kits allowed prompt and specific results, validated by the use of an internal control. The Nanogen kit showed the best clinical sensitivity.
Touati
A
A
Service des Laboratoires, Centre National de Greffe de Moelle Osseuse, Tunis, Tunisia.
Benard
A
A
Hassen
A Ben
AB
Bébéar
C M
CM
Pereyre
S
S
D006801:Humans; D025202:Molecular Diagnostic Techniques / Q000379:methods; D009177:Mycoplasma pneumoniae / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000379:methods*; D011933:Reagent Kits, Diagnostic; D012137:Respiratory System / Q000382:microbiology*; D012680:Sensitivity and Specificity
D011933:Reagent Kits, Diagnostic