[Cloning and quantitative expression of chlorocatechol 1,2-dioxygenase gene].
The chlorocatechol 1,2-dioxygenase gene, designated as tcbC (J5-2) (GenBank Accession No. EF111021), was cloned from total DNA of Pseudomonas aeruginosa J5-2 which can degrade 1,2,4-trichlorobenzene. The deduced amino acid sequence analysis of tcbC (JS-2) shows that it is different from tcbC (P51) cloned from another 1,2,4-trichlorobenzene-degrader Pseudomonas sp. P51. tcbC (J5-2) is only 95% similar to tcbC (P51) at the amino acid level.Three critical amino acid residues in tcbC (J5-1) are Leu-48, Ala-52, and Ile-73. Otherwise, they are Val-48, Ala-52, and Met-73 in tcbC (P51). mRNA accumulation of tcbC in J5-2 was quantified by real-time reverse transcription-PCR, when J5-2 grew on 1,2-dichlorobenzene, 1,3-dichlorobensene and 1,2,4-trichlorobenzene respectively. The results show that 1,2, 4-trichlorobenzen is a better inducer than 1,2- and 1,3-dichlorobensene.
Song
Lei
L
College of Architecture and Civil Engineering, Inner Mongolia University of Technology, Huhhot 010051, China. songlei2002@tsinghua.org.cn
Wang
Hui
H
Shi
Han-Chang
HC
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D002722:Chlorobenzenes / Q000378:metabolism*; D003001:Cloning, Molecular; D049308:Dioxygenases / Q000235:genetics* / Q000378:metabolism; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D011550:Pseudomonas aeruginosa / Q000201:enzymology* / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism
D001426:Bacterial Proteins; D002722:Chlorobenzenes; D012333:RNA, Messenger; C009947:1,2,4-trichlorobenzene; C004726:2-dichlorobenzene; C042146:1,3-dichlorobenzene; D049308:Dioxygenases; C044173:chlorocatechol 1,2-dioxygenase