title: In situ microextraction method to determine the viscosity of biofluid in threadlike structures on the surfaces of mammalian organs.
abstract: We report a method to measure the viscosity of microL volumes of biofluid obtained from threadlike structures (NTSs) on the surfaces of mammalian (rabbit) internal organs. The fluid was mechanically microextracted in situ from NTSs on the organ surfaces by a glass capillary connected to an extractor. From the Brownian motion of the 0.8+/-0.1microm diameter granules in the extracted fluid, the fluid viscosity was determined to be 1.4+/-0.1mPa s at room temperature. This viscosity is comparable to the viscosity of rabbit blood plasma.
authors.affiliation: Biomedical Physics Laboratory, Department of Physics and Astronomy, Seoul National University, Seoul 151-747, Korea.
authors.forename: Baeckkyoung
authors.initials: B
authors.lastname: Sung
authors.forename: Min Su
authors.initials: MS
authors.lastname: Kim
authors.forename: Adam
authors.initials: A
authors.lastname: Corrigan
authors.forename: Athene M
authors.initials: AM
authors.lastname: Donald
authors.forename: Kwang-Sup
authors.initials: KS
authors.lastname: Soh
mesh_terms: D000818:Animals; D001826:Body Fluids / Q000737:chemistry*; D009391:Nephelometry and Turbidimetry / Q000379:methods*; D011817:Rabbits; D052617:Solid Phase Microextraction / Q000379:methods*; D013499:Surface Properties; D014781:Viscera / Q000502:physiology*; D014783:Viscosity