title: Design of liposome-based pH sensitive nanoSPIN probes: nano-sized particles with incorporated nitroxides.
abstract: Liposome-based nanoSized Particles with Incorporated Nitroxides, or nanoSPINs, were designed for EPR applications as pH probes in biological systems. Phospholipid membrane of the liposomes with incorporated gramicidin A showed selective permeability to a small analyte, H(+), while protecting entrapped sensing nitroxide from biological reductants. An application of the pH-sensitive nanoSPIN in an ischemia model in rat heart homogenate allows for monitoring ischemia-induced acidosis while protecting encapsulated nitroxide against bioreduction.
authors.affiliation: The Ohio State University Medical Center, Columbus, 43210, USA.
authors.forename: Yakov Y
authors.initials: YY
authors.lastname: Woldman
authors.forename: Sergey V
authors.initials: SV
authors.lastname: Semenov
authors.forename: Andrey A
authors.initials: AA
authors.lastname: Bobko
authors.forename: Igor A
authors.initials: IA
authors.lastname: Kirilyuk
authors.forename: Julya F
authors.initials: JF
authors.lastname: Polienko
authors.forename: Maxim A
authors.initials: MA
authors.lastname: Voinov
authors.forename: Elena G
authors.initials: EG
authors.lastname: Bagryanskaya
authors.forename: Valery V
authors.initials: VV
authors.lastname: Khramtsov
mesh_terms: D000138:Acidosis; D000818:Animals; D055664:Electrochemical Techniques; D004578:Electron Spin Resonance Spectroscopy / Q000379:methods; D006096:Gramicidin / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D007511:Ischemia; D008081:Liposomes / Q000737:chemistry*; D015394:Molecular Structure; D009206:Myocardium / Q000378:metabolism; D053758:Nanoparticles / Q000737:chemistry*; D009589:Nitrogen Oxides / Q000737:chemistry*; D010101:Oxygen Consumption; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D008081:Liposomes; D009589:Nitrogen Oxides; D006096:Gramicidin