Discovery of antibody biomarkers using protein microarrays of tumor antigens cloned in high throughput.
Development of humoral and cellular immunity against self-cellular proteins in cancer patients is a phenomenal observation. The ability of immune system to sense the presence of the disease and to fight of the disease by generating autoantibodies against tumor antigens makes it a natural biosensor. Several screening technologies have been employed for the identification of tumor-specific antibodies in cancer patients. We have developed a multidimensional approach for the identification of diagnostic antigens that utilizes a combination of high-throughput antigen cloning and protein microarray-based serological detection of complex panels of antigens by exploiting the serum autoantibody repertoire directed toward tumor-associated antigens in cancer patients. Furthermore, validation of these antigens by different bioinformatics and biological approaches will reveal the diagnostic/prognostic utility of these antigens for personalized immunotherapy.
Chatterjee
Madhumita
M
Department of Molecular Biology and Genetics, Barbara Ann Karmanos Cancer Institute, Wayne State University, Detroit, MI, USA.
Wojciechowski
Jerzy
J
Tainsky
Michael A
MA
D000912:Antibodies, Neoplasm / Q000032:analysis*; D000951:Antigens, Neoplasm / Q000032:analysis*; D017123:Bacteriophage T7; D001483:Base Sequence; D014408:Biomarkers, Tumor / Q000032:analysis*; D015152:Blotting, Northern; D003001:Cloning, Molecular; D006801:Humans; D008969:Molecular Sequence Data; D019151:Peptide Library; D011061:Poly A; D040081:Protein Array Analysis / Q000379:methods*; D012333:RNA, Messenger / Q000302:isolation & purification; D012334:RNA, Neoplasm / Q000235:genetics / Q000302:isolation & purification; D044967:Serum
D000912:Antibodies, Neoplasm; D000951:Antigens, Neoplasm; D014408:Biomarkers, Tumor; D019151:Peptide Library; D012333:RNA, Messenger; D012334:RNA, Neoplasm; D011061:Poly A