title: Indoleamine 2,3-dioxygenase is expressed in the endometrium of cycling mice throughout the oestrous cycle.
abstract: Indoleamine 2,3-dioxygenase (IDO), an enzyme responsible for tryptophan catabolism, is thought to be required to prevent the rejection of the allogenic fetus by maternal T cells and to protect against intra- and extra-cellular pathogens. Consequently, we studied the expression of IDO in the endometrium of female Balb/c mice during the oestrous cycle. At each phase, the endometrium was peeled away and the relative expression of IDO mRNA was detected by semi-quantitative RT-PCR. The presence of IDO protein was confirmed in each phase by Western blotting and immunohistochemistry. Our results showed that IDO is expressed in the endometrium of cycling mice during all the phases of oestrous cycle. The expression of IDO was highest at the oestrus and lowest at the dioestrus. By means of Western blotting and immunohistochemistry, we obtained evidence that IDO protein is synthesised in the endometrium of cycling mice throughout the oestrous cycle. In accordance with RT-PCR results, IDO protein was predominant at the oestrus phase. IDO protein was mainly localised in the glandular and luminal epithelial cells. Our results support the concept of IDO providing a mechanism of innate immunity to protect from ascending infections of the female reproductive tract. In addition, considering the fact that mating only occurs during the oestrus phase, the high expression of IDO in this phase is likely to be a mechanism that induces immunological tolerance of the fetus.
authors.affiliation: Monoclonal Antibody Research Center, Avicenna Research Institute, Tehran, Iran.
authors.forename: M
authors.initials: M
authors.lastname: Jeddi-Tehrani
authors.forename: N
authors.initials: N
authors.lastname: Abbasi
authors.forename: P
authors.initials: P
authors.lastname: Dokouhaki
authors.forename: J
authors.initials: J
authors.lastname: Ghasemi
authors.forename: S
authors.initials: S
authors.lastname: Rezania
authors.forename: M
authors.initials: M
authors.lastname: Ostadkarampour
authors.forename: H
authors.initials: H
authors.lastname: Rabbani
authors.forename: M A
authors.initials: MA
authors.lastname: Akhondi
authors.forename: Z Tahmasebi
authors.initials: ZT
authors.lastname: Fard
authors.forename: A H
authors.initials: AH
authors.lastname: Zarnani
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D004717:Endometrium / Q000276:immunology / Q000378:metabolism*; D004847:Epithelial Cells / Q000276:immunology / Q000378:metabolism*; D005260:Female; D007113:Immunity, Innate; D007120:Immunochemistry; D050503:Indoleamine-Pyrrole 2,3,-Dioxygenase / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D008597:Menstrual Cycle / Q000276:immunology / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D020133:Reverse Transcriptase Polymerase Chain Reaction; D023001:Transplantation Tolerance; D014364:Tryptophan / Q000378:metabolism
chemical_list: D050503:Indoleamine-Pyrrole 2,3,-Dioxygenase; D014364:Tryptophan