title: Alteromonas halophila sp. nov., a new moderately halophilic bacterium isolated from a sea anemone.
abstract: A pale yellow-colored, moderately halophilic, Gram-negative, catalase- and oxidase-positive, non-sporulating, rod-shaped, motile, aerobic bacterium, designated strain JSM 073008(T), was isolated from a sea anemone (Anthopleura xanthogrammica) collected from Naozhou Island, Leizhou Bay, South China Sea. The organism was able to grow with 1-20% (w/v) total salts (optimum, 5-10%), at pH 6.0-10.0 (optimum, pH 7.5) and 10-40 degrees C (optimum, 25-30 degrees C). The major cellular fatty acids were C(16:0), C(16:1) omega7c/iso-C(15:0) 2-OH and C(18:1) omega7c. The polar lipids consisted of phosphatidylethanolamine, phosphatidylglycerol and an unidentified phospholipid. The predominant respiratory quinone was Q-8 and the genomic DNA G + C content was 47.5 mol%. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain JSM 073008(T) should be assigned to the genus Alteromonas, being most closely related to Alteromonas hispanica F-32(T) (sequence similarity 96.9%), followed by Alteromonas genovensis LMG 24078(T) (96.6%) and Alteromonas litorea TF-22(T) (96.4%). The sequence similarities between the novel isolate and the type strains of other recognized Alteromonas species ranged from 95.9% (with Alteromonas stellipolaris ANT 69a(T)) to 94.5% (with Alteromonas simiduii BCRC 17572(T)). The combination of phylogenetic analysis, phenotypic characteristics and chemotaxonomic data supported the view that strain JSM 073008(T) represents a new species of the genus Alteromonas, for which the name Alteromonas halophila sp. nov. is proposed. The type strain is JSM 073008(T) (=CCTCC AA 207035(T) = KCTC 22164(T)).
authors.affiliation: College of Biology and Environmental Science, Department of Financial Management, Jishou University, 416000 Jishou, People's Republic of China.
authors.forename: Yi-Guang
authors.initials: YG
authors.lastname: Chen
authors.forename: Huai-Dong
authors.initials: HD
authors.lastname: Xiao
authors.forename: Shu-Kun
authors.initials: SK
authors.lastname: Tang
authors.forename: Yu-Qin
authors.initials: YQ
authors.lastname: Zhang
authors.forename: Entomack
authors.initials: E
authors.lastname: Borrathybay
authors.forename: Xiao-Long
authors.initials: XL
authors.lastname: Cui
authors.forename: Wen-Jun
authors.initials: WJ
authors.lastname: Li
authors.forename: Yan-Qi
authors.initials: YQ
authors.lastname: Liu
mesh_terms: D020591:Alteromonas / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D000818:Animals; D002681:China; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012615:Sea Anemones / Q000382:microbiology*; D012623:Seawater / Q000382:microbiology*; D012965:Sodium Chloride / Q000378:metabolism*
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D012965:Sodium Chloride