Dietary omega-3 fatty acids attenuate cellular damage after a hippocampal ischemic insult in adult rats.
The role of omega-3 polyunsaturated fatty acids (3PUFAs) on brain function is increasingly demonstrated. Here, the effect of dietary deprivation of essential 3PUFAs on some parameters related to neuroprotection was investigated. Rats were fed with two different diets: omega-3 diet and omega-3-deprived diet. To assess the influence of 3PUFAs on brain responses to ischemic insult, hippocampal slices were subjected to an oxygen and glucose deprivation (OGD) model of in vitro ischemia. The omega-3-deprived group showed higher cell damage and stronger decrease in the [(3)H]glutamate uptake after OGD. Moreover, omega-3 deprivation influenced antiapoptotic cell response after OGD, affecting GSK-3beta and ERK1/2, but not Akt, phosphorylation. Taken together, these results suggest that 3PUFAs are important for cell protection after ischemia and also seem to play an important role in the activation of antiapoptotic signaling pathways.
Moreira
Júlia D
JD
Biochemistry Department, Biochemistry Postgraduate Program, ICBS, Federal University of Rio Grande do Sul, 90035-003 Porto Alegre, RS, Brazil. juliamoreira@gmail.com.br
Knorr
Luisa
L
Thomazi
Ana Paula
AP
Simão
Fabrício
F
Battú
Cíntia
C
Oses
Jean Pierre
JP
Gottfried
Carmem
C
Wofchuk
Susana
S
Salbego
Christianne
C
Souza
Diogo O
DO
Perry
Marcos L S
ML
Vinadé
Lúcia
L
D000818:Animals; D016923:Cell Death; D015687:Cell Hypoxia; D004042:Dietary Fats, Unsaturated / Q000008:administration & dosage*; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism; D005228:Fatty Acids, Essential / Q000172:deficiency; D015525:Fatty Acids, Omega-3 / Q000008:administration & dosage* / Q000502:physiology; D005260:Female; D038362:Glycogen Synthase Kinase 3 / Q000378:metabolism; D000071679:Glycogen Synthase Kinase 3 beta; D006624:Hippocampus / Q000378:metabolism / Q000503:physiopathology*; D020925:Hypoxia-Ischemia, Brain / Q000378:metabolism / Q000503:physiopathology*; D066298:In Vitro Techniques; D008297:Male; D018696:Neuroprotective Agents / Q000008:administration & dosage*; D010766:Phosphorylation; D011499:Protein Processing, Post-Translational; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000378:metabolism / Q000517:prevention & control*; D015398:Signal Transduction
D004042:Dietary Fats, Unsaturated; D005228:Fatty Acids, Essential; D015525:Fatty Acids, Omega-3; D018696:Neuroprotective Agents; D000071679:Glycogen Synthase Kinase 3 beta; C000605508:Gsk3b protein, rat; D048049:Extracellular Signal-Regulated MAP Kinases; D038362:Glycogen Synthase Kinase 3