Equine thrombospondin II and secreted protein acidic and cysteine-rich in a model of normal and pathological wound repair.
Wound healing in horses is complicated, particularly when wounds are on the limb. The objectives of this study were to clone equine thrombospondin II (THBS2) and secreted protein acidic and cysteine-rich (SPARC) cDNAs and to compare the spatiotemporal expression of mRNAs and proteins during repair of body and limb wounds. These molecules were targeted in view of their potential biological contribution to angiogenesis, which is exacerbated during the repair of limb wounds in horses. Cloning was achieved by screening size-selected cDNA libraries previously derived from 7-day-old wounds. Expression was studied in unwounded skin and in samples from 1, 2, 3, 4, and 6 wk old wounds of the body and limb. Temporal gene expression was determined by semiquantitative RT-PCR, while protein expression was mapped immunohistochemically. The temporal pattern of expression for both genes was similar; wounding caused immediate upregulation of mRNA, which did not return to baseline by the end of the study, and overexpression was noted in body relative to limb wounds. Immunostaining for THBS2 and SPARC was induced by wounding, though no differences in stain location or intensity were detected between body and limb wounds. This study is the first to characterize equine cDNA for THBS2 and SPARC and to document mRNA expression over the different phases of repair. THBS2 and SPARC might modulate angiogenesis during wound healing in the horse, which could protect against the disproportionate fibroplasia commonly afflicting limb wounds and leading to the development of exuberant granulation tissue.
Miragliotta
Vincenzo
V
Department of Veterinary Anatomy, Biochemistry and Physiology, University of Pisa, Pisa, Italy.
Raphaël
Kevin
K
Ipiña
Zoë
Z
Lussier
Jacques G
JG
Theoret
Christine L
CL
D000818:Animals; D015815:Cell Adhesion Molecules / Q000235:genetics / Q000378:metabolism*; D003001:Cloning, Molecular; D017931:DNA Primers / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics; D006736:Horses; D015151:Immunoblotting; D007150:Immunohistochemistry; D016014:Linear Models; D018919:Neovascularization, Physiologic / Q000235:genetics / Q000502:physiology; D015676:Osteonectin / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D019699:Thrombospondins / Q000235:genetics / Q000378:metabolism*; D014945:Wound Healing / Q000502:physiology*; D000099262:Thrombospondin 2
D015815:Cell Adhesion Molecules; D017931:DNA Primers; D018076:DNA, Complementary; D015676:Osteonectin; D012333:RNA, Messenger; D019699:Thrombospondins; D000099262:Thrombospondin 2