A game with many players: control of gdh transcription in Corynebacterium glutamicum.
Glutamate dehydrogenase is a central enzyme connecting nitrogen and carbon metabolism via its precursors ammonium and oxoglutarate and its product glutamate. In Corynebacterium glutamicum glutamate dehydrogenase is especially important, since it is a key enzyme for the biotechnological production of the flavour enhancer L-glutamate. In this study, the regulation of gdh transcription was investigated. While originally described as a constitutively expressed gene, we could show that gdh transcription is highly variable depending on environmental conditions. Regulation of the gdh gene by the C. glutamicum nitrogen control protein AmtR was analyzed in detail in this study.
Hänssler
Eva
E
Lehrstuhl für Mikrobiologie, Friedrich-Alexander-Universität Erlangen-Nürnberg, Staudtstr. 5, 91058 Erlangen, Germany.
Müller
Tim
T
Palumbo
Katrin
K
Patek
Mirek
M
Brocker
Melanie
M
Krämer
Reinhard
R
Burkovski
Andreas
A
D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D048230:Corynebacterium glutamicum / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development; D024202:Electrophoretic Mobility Shift Assay; D015964:Gene Expression Regulation, Bacterial; D005969:Glutamate Dehydrogenase / Q000235:genetics* / Q000378:metabolism; D009584:Nitrogen / Q000378:metabolism; D011401:Promoter Regions, Genetic; D012097:Repressor Proteins / Q000378:metabolism; D013312:Stress, Physiological; D013696:Temperature; D014158:Transcription, Genetic
C414398:AmtR protein, Corynebacterium glutamicum; D001426:Bacterial Proteins; D012097:Repressor Proteins; D005969:Glutamate Dehydrogenase; D009584:Nitrogen