Supported bilayers formed from different phospholipids on spherical silica substrates.
Spherical supported bilayer membranes (SS-BLMs) are very attractive candidates in modern bioanalytics and biorecognition studies. A uniform, facile method of preparing different SS-BLMs on silica beads is reported. Confocal fluorescence microscopy and cryo-TEM imaging have been used to characterize these SS-BLMs. Thermal analysis data and FRAP experiments show that the bilayer properties of the SS-BLM are consistent with those of lipid vesicles from which they are formed. The possibility of modulating the size, lipid type and functionality, and mechanical stability makes these rigid liposomes very attractive candidates in biosensors, drug screening, and gene delivery-related applications. This is especially true in work with native vesicle membranes derived from living cells because the existing methods can only accommodate anionic membranes to a limited extent.
Gopalakrishnan
Gopakumar
G
Department of Chemistry, Montreal Neurological Institute & Hospital, and McGill Program in Neuroengineering, Centre for Self-Assembled Chemical Structures, Department of Anatomy & Dentistry, McGill University, Montreal, Canada.
Rouiller
Isabelle
I
Colman
David R
DR
Lennox
R Bruce
RB
D015374:Biosensing Techniques / Q000379:methods; D055252:Cell-Derived Microparticles / Q000737:chemistry; D004353:Drug Evaluation, Preclinical / Q000379:methods; D018014:Gene Transfer Techniques; D008051:Lipid Bilayers / Q000737:chemistry*; D010743:Phospholipids / Q000737:chemistry*; D012822:Silicon Dioxide / Q000737:chemistry*
D008051:Lipid Bilayers; D010743:Phospholipids; D012822:Silicon Dioxide