title: Porcine hypothalamic aromatase cytochrome P450: isoform characterization, sex-dependent activity, regional expression, and regulation by enzyme inhibition in neonatal boars.
abstract: Domestic pigs have three CYP19 genes encoding functional paralogues of the enzyme aromatase cytochrome P450 (P450arom) that are expressed in the gonads, placenta, and preimplantation blastocyst. All catalyze estrogen synthesis, but the gonadal-type enzyme is unique in also synthesizing a nonaromatizable biopotent testosterone metabolite, 1OH-testosterone (1OH-T). P450arom is expressed in the vertebrate brain, is higher in males than females, but has not been investigated in pigs, to our knowledge. Therefore, these studies defined which of the porcine CYP19 genes was expressed, and at what level, in adult male and female hypothalamus. Regional expression was examined in mature boars, and regulation of P450arom expression in neonatal boars was investigated by inhibition of P450arom with letrozole, which is known to reprogram testicular expression. Pig hypothalami expressed the gonadal form of P450arom (redesignated the "gonadal/hypothalamic" porcine CYP19 gene and paralogue) based on functional analysis confirmed by cloning and sequencing transcripts. Hypothalamic tissue synthesized 1OH-T and was sensitive to the selective P450arom inhibitor etomidate. Levels were 4-fold higher in male than female hypothalami, with expression in the medial preoptic area and lateral borders of the ventromedial hypothalamus of boars. In vivo, letrozole-treated neonates had increased aromatase activity in hypothalami but decreased activity in testes. Therefore, although the same CYP19 gene is expressed in both tissues, expression is regulated differently in the hypothalamus than testis. These investigations, the first such studies in pig brain to our knowledge, demonstrate unusual aspects of P450arom expression and regulation in the hypothalamus, offering promise of gaining better insight into roles of P450arom in reproductive function.
authors.affiliation: Department of Population Health & Reproduction, University of California Davis, Davis, California 95616, USA.
authors.forename: C J
authors.initials: CJ
authors.lastname: Corbin
authors.forename: T
authors.initials: T
authors.lastname: Berger
authors.forename: J J
authors.initials: JJ
authors.lastname: Ford
authors.forename: C E
authors.initials: CE
authors.lastname: Roselli
authors.forename: W
authors.initials: W
authors.lastname: Sienkiewicz
authors.forename: B C
authors.initials: BC
authors.lastname: Trainor
authors.forename: J F
authors.initials: JF
authors.lastname: Roser
authors.forename: J D
authors.initials: JD
authors.lastname: Vidal
authors.forename: N
authors.initials: N
authors.lastname: Harada
authors.forename: A J
authors.initials: AJ
authors.lastname: Conley
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D001141:Aromatase / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D047072:Aromatase Inhibitors / Q000378:metabolism / Q000494:pharmacology*; D001483:Base Sequence; D004958:Estradiol / Q000097:blood; D005045:Etomidate / Q000494:pharmacology*; D005260:Female; D015971:Gene Expression Regulation, Enzymologic; D006066:Gonads / Q000201:enzymology; D007031:Hypothalamus / Q000033:anatomy & histology / Q000187:drug effects / Q000201:enzymology*; D007527:Isoenzymes / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D000077289:Letrozole; D008297:Male; D008861:Microsomes / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism; D008969:Molecular Sequence Data; D009570:Nitriles / Q000494:pharmacology*; D010902:Pituitary Gland / Q000201:enzymology; D010920:Placenta / Q000201:enzymology; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D016415:Sequence Alignment; D012727:Sex Characteristics; D018709:Statistics, Nonparametric; D034421:Sus scrofa / Q000254:growth & development / Q000378:metabolism*; D013737:Testis / Q000187:drug effects / Q000201:enzymology; D013739:Testosterone / Q000097:blood; D014230:Triazoles / Q000494:pharmacology*
chemical_list: D047072:Aromatase Inhibitors; D007527:Isoenzymes; D009570:Nitriles; D011993:Recombinant Fusion Proteins; D014230:Triazoles; D013739:Testosterone; D004958:Estradiol; D000077289:Letrozole; D001141:Aromatase; D005045:Etomidate