PCR-SSCP and sequence analysis of three Odontotermes spp. (order: isoptera; family: termitidae) on the basis of partial 16SrRNA gene.
Partial 16S gene fragments were amplified by using specific primers in few species/populations of termites of the genus Odontotermes (Isoptera:Termitidae:Macrotermitinae), and the PCR products were subjected to SSCP analysis. Three haplotypes obtained were subjected to sequencing. The sequences obtained were characterized to see the frequencies of each nucleotide bases. High A + T content was observed. The inter-specific pairwise sequence divergence in Odontotermes spp. ranged from 0% to 4.8% across the entire 16S gene fragment. Identical sequences were found between two populations of O. horni. Individuals of different species having Type-I conformational pattern, i.e. O. obesus (-AI) and O. horni (-MI), as well as Type-II of O. obesus (-UII) and O. bhagwatii (-CHII) had no percent diversity. Phylogenetic trees drawn on the basis of distance Neighbour-joining method revealed clustering of individuals according to their genera and families.
Kumari
Mamtesh
M
Department of Zoology, Punjab University, Chandigarh, India.
Sharma
Vijay Lakshmi
VL
Sodhi
Monika
M
Mukesh
Manishi
M
Shouche
Yogesh
Y
Sobti
Ranbir Chander
RC
D000818:Animals; D001482:Base Composition; D006239:Haplotypes; D020049:Isoptera / Q000235:genetics*; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D018807:Polymorphism, Single-Stranded Conformational; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D017422:Sequence Analysis, DNA; D013045:Species Specificity
D012336:RNA, Ribosomal, 16S