The impact of PEPC phosphorylation on growth and development of Arabidopsis thaliana: molecular and physiological characterization of PEPC kinase mutants.
Two phosphoenolpyruvate carboxylase (PEPC) kinase genes (PPCk1 and PPCk2) are present in the Arabidopsis genome; only PPCk1 is expressed in rosette leaves. Homozygous lines of two independent PPCk1 T-DNA-insertional mutants showed very little (dln1), or no (csi8) light-induced PEPC phosphorylation and a clear retard in growth under our greenhouse conditions. A mass-spectrometry-based analysis revealed significant changes in metabolite profiles. However, the anaplerotic pathway initiated by PEPC was only moderately altered. These data establish the PPCk1 gene product as responsible for leaf PEPC phosphorylation in planta and show that the absence of PEPC phosphorylation has pleiotropic consequences on plant metabolism.
Meimoun
Patrice
P
Institut de Biotechnologie des Plantes, Centre National de la Recherche Scientifique Unité Mixte de Recherche 8618, Université Paris-Sud 11, Orsay Cedex, France.
Gousset-Dupont
Aurélie
A
Lebouteiller
Bénédicte
B
Ambard-Bretteville
Françoise
F
Besin
Evelyne
E
Lelarge
Caroline
C
Mauve
Caroline
C
Hodges
Michael
M
Vidal
Jean
J
D017360:Arabidopsis / Q000201:enzymology* / Q000254:growth & development*; D029681:Arabidopsis Proteins / Q000235:genetics* / Q000378:metabolism; D004269:DNA, Bacterial / Q000378:metabolism; D017343:Genes, Plant; D013058:Mass Spectrometry; D009154:Mutation; D010730:Phosphoenolpyruvate Carboxylase / Q000235:genetics / Q000378:metabolism*; D010766:Phosphorylation; D018515:Plant Leaves / Q000378:metabolism; D017346:Protein Serine-Threonine Kinases / Q000235:genetics* / Q000378:metabolism
D029681:Arabidopsis Proteins; D004269:DNA, Bacterial; C036483:T-DNA; C069767:phosphoenolpyruvate carboxylase kinase; D017346:Protein Serine-Threonine Kinases; D010730:Phosphoenolpyruvate Carboxylase