Identification of cervidae DNA in feedstuff using a real-time polymerase chain reaction method with the new fluorescence intercalating dye EvaGreen.
A real-time qualitative and quantitative polymerase chain reaction method (cer-194) using the fluorescence dye EvaGreen and aimed at the cytochrome b sequence was established for detection of cervidae DNA in feedstuff. Eight meat meal samples derived from deer, bovine, ovine, camel, pig, rabbit, fish, and chicken and 17 cervidae hair samples covering 2 subfamilies, 4 genera, and 7 species were tested to prove the specificity of the cer-194 system and its universality within the cervidae family. Detection limit of 0.1% deer meat in fish meal, blood powder, and feather powder matrixes was confirmed.
Chen
Ying
Y
Institute of Food Safety, Chinese Academy of Inspection and Quarantine, Beijing 100123, People's Republic of China. yqychen@yahoo.com.cn
Wu
Yajun
Y
Wang
Jing
J
Xu
Baoliang
B
Zhong
Zhengyu
Z
Xia
Jingshi
J
D000818:Animals; D001483:Base Sequence; D002645:Chickens; D045303:Cytochromes b / Q000235:genetics; D004247:DNA / Q000032:analysis*; D017931:DNA Primers; D003670:Deer / Q000235:genetics*; D005504:Food Analysis; D005506:Food Contamination; D008460:Meat / Q000592:standards*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D016415:Sequence Alignment; D013050:Spectrometry, Fluorescence / Q000379:methods
D017931:DNA Primers; D004247:DNA; D045303:Cytochromes b