title: Blastocystis: unravelling potential risk factors and clinical significance of a common but neglected parasite.
abstract: Two independent studies were conducted to describe symptoms and potential risk factors associated with Blastocystis infection. Isolates were subtyped by molecular analysis. In the NORMAT study (126 individuals randomly sampled from the general population) 24 (19%) were positive for Blastocystis. Blastocystis was associated with irritable bowel syndrome (P=0.04), contact with pigs (P<0.01) and poultry (P=0.03). In the Follow-up (FU) study (follow-up of 92 Blastocystis-positive patients), reports on bloating were associated with subtype (ST) 2 (P<0.01), and blood in stool to mixed subtype infection (P=0.06). ST1 was more common in FU individuals (32%) than in NORMAT individuals (8%), whereas single subtype infections due to ST3 or ST4 were seen in 63% of the NORMAT cases and 28% of the FU cases. Only FU individuals hosted ST7, and ST6/7 infections due to ST7 or ST9 were characterized by multiple intestinal symptoms. The data indicate subtype-dependent differences in the clinical significance of Blastocystis.
authors.affiliation: Department of Bacteriology, Mycology and Parasitology, Statens Serum Institut, Copenhagen, Denmark. RUN@ssi.dk
authors.forename: C R
authors.initials: CR
authors.lastname: Stensvold
authors.forename: H C
authors.initials: HC
authors.lastname: Lewis
authors.forename: A M
authors.initials: AM
authors.lastname: Hammerum
authors.forename: L J
authors.initials: LJ
authors.lastname: Porsbo
authors.forename: S S
authors.initials: SS
authors.lastname: Nielsen
authors.forename: K E P
authors.initials: KE
authors.lastname: Olsen
authors.forename: M C
authors.initials: MC
authors.lastname: Arendrup
authors.forename: H V
authors.initials: HV
authors.lastname: Nielsen
authors.forename: K
authors.initials: K
authors.lastname: Mølbak
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000818:Animals; D000977:Antiparasitic Agents / Q000627:therapeutic use; D016844:Blastocystis / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D016776:Blastocystis Infections / Q000150:complications / Q000188:drug therapy / Q000453:epidemiology*; D002648:Child; D002675:Child, Preschool; D003718:Denmark / Q000453:epidemiology; D004029:Dientamoeba / Q000302:isolation & purification; D004030:Dientamoebiasis / Q000150:complications / Q000188:drug therapy / Q000453:epidemiology*; D005260:Female; D005500:Follow-Up Studies; D005838:Genotype; D006801:Humans; D007223:Infant; D043183:Irritable Bowel Syndrome / Q000469:parasitology; D008297:Male; D008795:Metronidazole / Q000627:therapeutic use; D008875:Middle Aged; D011159:Population Surveillance; D015995:Prevalence; D017211:Treatment Failure; D055815:Young Adult
chemical_list: D000977:Antiparasitic Agents; D008795:Metronidazole