title: Linker histone-like proteins in Muscovy duck (Cairina moschata L) erythrocyte chromatin.
abstract: Linker Histone-Like proteins (LHL1 and LHL2) were identified within a linker histone complement of Muscovy duck erythrocyte chromatin. Polyacrylamide gel electrophoretic patterns of N-bromosuccinimide-cleaved LHL products as well as liquid chromatography-electrospray-ion trap mass spectrometry analyses of trypsin-digested LHL peptides revealed structural similarity of LHL1 to histone H5 and between LHL2 and histone H1 subtypes. Since the LHL proteins were stable in the presence of 2-mercaptoethanol and dithiothreitol that reduce disulfide bonds, it appeared unlikely that this doublet was a thiol-derived product of linker histones. A loss of LHL1, with a concomitant maintenance of LHL2 after treatment with dilute alkali, seems to suggest that they might represent disparate protein conjugates resulting from linker histone modifications through ester linkages.
authors.affiliation: Department of Biochemistry and Genetics, Jan Kochanowski University, Kielce, Poland. a.kowalski@ujk.kielce.pl
authors.forename: Andrzej
authors.initials: A
authors.lastname: Kowalski
authors.forename: Jan
authors.initials: J
authors.lastname: Pałyga
authors.forename: Ewa
authors.initials: E
authors.lastname: Górnicka-Michalska
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002843:Chromatin / Q000737:chemistry*; D002853:Chromatography, Liquid; D004372:Ducks; D004591:Electrophoresis, Polyacrylamide Gel; D004912:Erythrocytes / Q000737:chemistry*; D006657:Histones / Q000737:chemistry*; D008969:Molecular Sequence Data; D020033:Protein Isoforms / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D002843:Chromatin; D006657:Histones; D020033:Protein Isoforms