title: Use of chitosan membrane associated with polypropylene mesh to prevent peritoneal adhesion in rats.
abstract: The correction of wall abdominal defects often requires the use of implants such as polypropylene meshes. In spite of presenting good tissue acceptance, these biomaterials can migrate to adjacent viscera, promote enterocutaneos fistulas, tissue adherence and visceral erosions. In this work, the barrier effect of chitosan films associated with polypropylene meshes on adhesion formation experimentally induced in Wistar rats was evaluated. The animals were divided into two groups with 10 animals each. Animals in the CPP group were implanted with chitosan films associated with polypropylene meshes, whereas the ones in the PP group received only polypropylene meshes. After 8 days, the animals were submitted to euthanasia using CO(2) and a descriptive study focusing adhesion formation, visceral involvement with sutures and mesh peritonization was performed. Also, subimplanted material was collected for histopathology analysis. The results showed that the CPP group presented weak adhesions to the omentum over the stitch knots in eight animals. In all animals, the meshes were peritonized, not allowing their visualization after removing the chitosan films. In the PP group, six animals presented intestinal adhesions to the meshes and, in one of them, hepatic adhesion to the mesh was observed, besides omentum adhesion on more than 50% of the mesh area. The protective effect of chitosan films when sutured over polypropylene meshes, as well as no exacerbation of inflammation associated to the peritoneal lesions was statistically demonstrated. Therefore, chitosan films can indeed minimize the formation of peritoneal adhesions induced by polypropylene meshes in rats.
authors.affiliation: Department of Veterinary Medicine, School of Veterinary Medicine, Federal University of Goiás, CEP 74001-970 Goiânia, Goiás, Brazil. nmp@vet.ufg.br
authors.forename: Neusa Margarida
authors.initials: NM
authors.lastname: Paulo
authors.forename: Marcelo Seixo
authors.initials: MS
authors.lastname: de Brito e Silva
authors.forename: Angela Maria
authors.initials: AM
authors.lastname: Moraes
authors.forename: Ana Paula
authors.initials: AP
authors.lastname: Rodrigues
authors.forename: Liliana Borges
authors.initials: LB
authors.lastname: de Menezes
authors.forename: Marina Pacheco
authors.initials: MP
authors.lastname: Miguel
authors.forename: Flávia Gontijo
authors.initials: FG
authors.lastname: de Lima
authors.forename: Aline
authors.initials: A
authors.lastname: de Morais Faria
authors.forename: Lívia Maria Lindoso
authors.initials: LM
authors.lastname: Lima
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry / Q000378:metabolism; D048271:Chitosan / Q000737:chemistry / Q000378:metabolism; D020007:Implants, Experimental; D008422:Materials Testing; D009852:Omentum / Q000473:pathology / Q000601:surgery; D010537:Peritoneum / Q000473:pathology / Q000601:surgery; D011126:Polypropylenes / Q000737:chemistry / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D013526:Surgical Mesh; D000267:Tissue Adhesions / Q000517:prevention & control*
chemical_list: D001672:Biocompatible Materials; D011126:Polypropylenes; D048271:Chitosan