title: The expression pattern of TIR8 is conserved among vertebrates.
abstract: IL-1R/TLRs play an important role in the inflammatory responses. Their signaling pathway is tightly regulated to keep inflammation under control. The regulation involves both extracellular and intracellular mechanisms. TIR8, also known as SIGIRR (Single Immunoglobulin IL-1R-Related molecule), is an orphan receptor belonging to the IL-1R/TLRs super-family. Recent studies suggest its role in modulating the inflammatory response in the gastrointestinal tract in mice, acting as an IL-1 receptor family antagonist. We identified the sequence and analyzed the expression of TIR8 in a wide panel of organs and tissues in different animals. There was high homology of the cDNA sequence among human, mouse and the domestic species (74-85%). The pattern of expression of this receptor was similar in all the species examined (high levels in kidney and gastrointestinal tract) and similar to the human. These results demonstrate that TIR8 is conserved, in evolutionary terms, both with regard to sequence and pattern of expression, a finding consistent with a key function of this molecule in modulating inflammation in the gastrointestinal tract.
authors.affiliation: Dipartimento di Patologia Animale, Igiene e Sanità Pubblica Veterinaria, Università di Milano, 20133 Milano, Italy. federica.riva@unimi.it
authors.forename: Federica
authors.initials: F
authors.lastname: Riva
authors.forename: Nadia
authors.initials: N
authors.lastname: Polentarutti
authors.forename: Giulia
authors.initials: G
authors.lastname: Tribbioli
authors.forename: Alberto
authors.initials: A
authors.lastname: Mantovani
authors.forename: Cecilia
authors.initials: C
authors.lastname: Garlanda
authors.forename: Lauretta
authors.initials: L
authors.lastname: Turin
mesh_terms: D000818:Animals; D015152:Blotting, Northern; D002417:Cattle; D002645:Chickens; D018076:DNA, Complementary / Q000737:chemistry; D004285:Dogs; D020869:Gene Expression Profiling; D006736:Horses; D006801:Humans; D051379:Mice; D017472:Receptors, Interleukin-1 / Q000737:chemistry / Q000235:genetics*; D013552:Swine
chemical_list: D018076:DNA, Complementary; D017472:Receptors, Interleukin-1