title: [Small interference RNA targeting nuclear factor-kappaB inhibits endometriotic angiogenesis in chick embryo chorioallantocic membrane].
abstract: OBJECTIVE
  To investigate the effect of small interference RNA (siRNA) targeting nuclear factor-kappaB (NF-kappaB) on endometriosis.
  
  
  METHODS
  The eutopic endometrium of women with endometriosis were transplanted into the nonvascular region of 8-day-old chicken embryo chorioallantocic membrane (CAM), and the effects of NF-kappaB p65 siRNA on the vascularization and endometriotic lesion formation were tested with proper controls.
  
  
  RESULTS
  Transplantation of the endometrium onto the CAM resulted in a strong angiogenic response in the chicken tissue. The angiogenesis was significantly reduced and endometriotic lesion formation significantly suppressed with siRNA targeting NF-kappaB in comparison with the control group.
  
  
  CONCLUSIONS
  The NF-kappaB pathway is involved in the development of endometriotic lesions in vitro, and NF-kappaB gene silencing reduces endometriotic angiogenesis and promotes cell apoptosis in the endometriotic lesions, suggesting that NF-kappaB might be a good target for endometriosis treatment.
authors.affiliation: Department of Obstetrics and Gynecology, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, China. liumb1972@126.com
authors.forename: Mu-biao
authors.initials: MB
authors.lastname: Liu
authors.forename: Yuan-li
authors.initials: YL
authors.lastname: He
authors.forename: Jie
authors.initials: J
authors.lastname: Zhong
mesh_terms: D000818:Animals; D002642:Chick Embryo; D049033:Chorioallantoic Membrane / Q000098:blood supply / Q000378:metabolism*; D004715:Endometriosis / Q000235:genetics* / Q000503:physiopathology*; D005260:Female; D006801:Humans; D016328:NF-kappa B / Q000172:deficiency* / Q000235:genetics*; D009389:Neovascularization, Pathologic / Q000235:genetics*; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics*
chemical_list: D016328:NF-kappa B; D034741:RNA, Small Interfering