Antinociceptive activity of Paederosidic Acid Methyl Ester (PAME) from the n-butanol fraction of Paederia scandens in mice.
Antinociceptive activity of Paederosidic Acid Methyl Ester (PAME), a chemical compound isolated from the n-butanol fraction of Paederia scandens, was evaluated in mice using chemical and thermal models of nociception. PAME given by intraperitoneal injection at doses of 20, 40 and 60 mg/kg produced significant inhibitions on chemical nociception induced by intraperitoneal acetic acid, subplantar formalin or capsaicin injections and on thermal nociception in the tail-flick test and the hot plate test. In the pentobarbital sodium-induced sleep time test and the open-field test, PAME neither significantly enhanced the pentobarbital sodium-induced sleep time nor impaired the motor performance, indicating that the observed antinociceptive activity of PAME was unlikely due to sedation or motor abnormality. Core body temperature measurement showed that PAME did not affect temperature within a 2-h period. Moreover, PAME-induced antinociception in the hot plate test was insensitive to naloxone or nimodipine but significantly antagonized by L-NAME (N (G)-nitro-L-arginine methyl ester), methylene blue and glibenclamide. These results suggested that PAME-produced antinociception was possibly related to the pathway of NO-cGMP-ATP sensitive K(+) channels, which merited further studies regarding the precise site and mechanisms of action.
Chen
Yu-Feng
YF
Department of Pharmacognosy, School of Pharmacy, Second Military Medical University, Shanghai, PR China.
Huang
Ying
Y
Tang
Wei-Zhong
WZ
Qin
Lu-Ping
LP
Zheng
Han-chen
HC
D020001:1-Butanol; D019342:Acetic Acid; D000700:Analgesics; D000701:Analgesics, Opioid / Q000494:pharmacology; D000818:Animals; D001831:Body Temperature / Q000187:drug effects; D002211:Capsaicin; D005557:Formaldehyde; D005960:Glucosides / Q000737:chemistry / Q000494:pharmacology*; D006358:Hot Temperature; D006993:Hypnotics and Sedatives / Q000494:pharmacology; D051379:Mice; D008813:Mice, Inbred ICR; D009020:Morphine / Q000494:pharmacology; D009043:Motor Activity / Q000187:drug effects; D010147:Pain Measurement / Q000187:drug effects; D010424:Pentobarbital / Q000494:pharmacology; D010936:Plant Extracts; D011930:Reaction Time / Q000187:drug effects; D019666:Rubiaceae / Q000737:chemistry*; D012890:Sleep / Q000187:drug effects; D012997:Solvents
D000700:Analgesics; D000701:Analgesics, Opioid; D005960:Glucosides; D006993:Hypnotics and Sedatives; D010936:Plant Extracts; D012997:Solvents; C543145:paederosidic acid methyl ester; D005557:Formaldehyde; D009020:Morphine; D020001:1-Butanol; D010424:Pentobarbital; D019342:Acetic Acid; D002211:Capsaicin