title: Methanolobus zinderi sp. nov., a methylotrophic methanogen isolated from a deep subsurface coal seam.
abstract: A methanogenic organism from the domain Archaea (SD1(T)) was isolated from saline water released from a coal seam located 926 m below the surface via a methane-producing well near Monroe, Louisiana, USA. Growth and methanogenesis were supported with methanol, monomethylamine, dimethylamine or trimethylamine, but not with dimethylsulfide, formate, acetate or H(2)/CO(2). Cells grew in high-salt minimal medium but growth was stimulated with yeast extract or tryptone. Cells were single, non-motile, irregular coccoids 0.5-1.0 microm in diameter and the cell wall contained protein. Conditions for the maximum rate of growth were 40-50 degrees C, 0.2-0.6 M NaCl, 100->or=200 mM MgCl(2), and pH 7.0-8.0. The G+C content of the genomic DNA was 42+/-1mol %. A comparison of 16S rRNA gene sequences indicated that strain SD1(T) was most closely related to Methanolobus oregonensis DSM 5435(T) with 96 % gene sequence similarity. It is proposed that strain SD1(T) represents a novel species, Methanolobus zinderi sp. nov. The type strain is SD1(T) (=ATCC BAA-1601(T)=DSM 21339(T)).
authors.affiliation: Department of Biochemistry and Molecular Biology, Pennsylvania State University, University Park, PA 16802, USA.
authors.forename: Sebastian N
authors.initials: SN
authors.lastname: Doerfert
authors.forename: Matthew
authors.initials: M
authors.lastname: Reichlen
authors.forename: Prabha
authors.initials: P
authors.lastname: Iyer
authors.forename: Mingyu
authors.initials: M
authors.lastname: Wang
authors.forename: James G
authors.initials: JG
authors.lastname: Ferry
mesh_terms: D000693:Anaerobiosis; D001482:Base Composition; D003031:Coal; D003470:Culture Media; D019641:DNA, Archaeal / Q000032:analysis / Q000302:isolation & purification; D019015:Geologic Sediments / Q000382:microbiology*; D008145:Louisiana; D008697:Methane / Q000378:metabolism*; D000432:Methanol / Q000378:metabolism; D017019:Methanosarcinaceae / Q000145:classification* / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012623:Seawater / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D013045:Species Specificity
chemical_list: D003031:Coal; D003470:Culture Media; D019641:DNA, Archaeal; D012336:RNA, Ribosomal, 16S; D008697:Methane; D000432:Methanol