[Study on preparation and quantity determination of Scrophularia ningpoensis dispensing granules].
OBJECTIVE
To develop the preparation method and to determine the quantity of effective constituents of Scrophularia ningpoensis dispensing granules.


METHODS
Nine experiments were carried out through L9 (3(4)) orthogonal design. The contents of harpagoside and cinnamic acid were determined by HPLC method.


RESULTS
The optimal extraction process was as follows: the slices were soaked in water in the ratio of 1:8 for 0.5 h, then they were decocted for 2 h. For the second time, 6 times of water was added and sustained for 1.5 h. Harpagoside and cinnamic acid were linear within the ranges of 0.0776-1.552 microg (r=0.9999) and 0.01446-0.4339 microg (r=0.9999), respectively. The average recoveries were 100.4% (RSD 1.98%, n=6) and 98.27% (RSD1.35%, n=6), respectively. Ten batches of granules were determined.


CONCLUSIONS
The extraction process is scientific and the determination method is suitable for quality control of Scrophularia ningpoensis dispensing granules.
Niu
Zhengrui
Z
Division of Pharmacognosy, School of Pharmaceutical Sciences, Peking University, Beijing 100083, China.
Wang
Rufeng
R
Jing
Yu
Y
Shang
Mingying
M
Cai
Shaoqing
S
D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D002934:Cinnamates / Q000032:analysis; D004339:Drug Compounding / Q000379:methods* / Q000592:standards; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage* / Q000737:chemistry*; D006027:Glycosides / Q000032:analysis; D016014:Linear Models; D011714:Pyrans / Q000032:analysis; D011786:Quality Control; D015203:Reproducibility of Results; D032266:Scrophularia / Q000737:chemistry*; D012680:Sensitivity and Specificity; D012997:Solvents / Q000737:chemistry; D013696:Temperature
D002934:Cinnamates; D004365:Drugs, Chinese Herbal; D006027:Glycosides; D011714:Pyrans; D012997:Solvents; C029010:cinnamic acid; C033249:harpagoside