title: [The effect of focal-adhesion micromanipulation in trauma and wound healing].
abstract: OBJECTIVE
  To explore the effect of focal-adhesion micromanipulation on the biological behavior of fibroblast.
  
  
  METHODS
  Micro-pot was made by microcontact printing. The molecules of constitutive protein was adhered on micro-pot by self-assemble of peptides. Skin fibroblasts were cultured on the membrane by self-made biomechanical cell culture for 2 weeks. Morphology observation and cell immunohistochemistry analysis was performed.
  
  
  RESULTS
  After 2 weeks, the morphology of the fibroblasts was diverse and more compliant. Cell immunohistochemistry analysis found that the expression of integrinbeta1, alpha5 and tensin was dramatically reduced.
  
  
  CONCLUSIONS
  The morphology and the biological behaviour of the fibroblasts in hypertrophic scar can be changed after micromanipulation of focal adhesion.
authors.affiliation: Department of Burn, Burn Institute of Shanghai, Ruijin Hospital, Shanghai Jiaotong University, Shanghai, China.
authors.forename: Yu-zhi
authors.initials: YZ
authors.lastname: Jiang
authors.forename: Jun
authors.initials: J
authors.lastname: Wei
authors.forename: Bo
authors.initials: B
authors.lastname: Yuan
authors.forename: Ying-kai
authors.initials: YK
authors.lastname: Liu
authors.forename: Zhi-yong
authors.initials: ZY
authors.lastname: Wang
authors.forename: Kui-fu
authors.initials: KF
authors.lastname: Ding
authors.forename: Xi-qiao
authors.initials: XQ
authors.lastname: Wang
authors.forename: Ming
authors.initials: M
authors.lastname: Tian
authors.forename: Chun-sen
authors.initials: CS
authors.lastname: Li
authors.forename: Fei
authors.initials: F
authors.lastname: Song
authors.forename: Chun
authors.initials: C
authors.lastname: Qing
authors.forename: Shu-liang
authors.initials: SL
authors.lastname: Lu
mesh_terms: D018929:Cell Culture Techniques; D048708:Cell Growth Processes; D002478:Cells, Cultured; D002921:Cicatrix / Q000601:surgery*; D020405:Dermis / Q000166:cytology; D005260:Female; D005347:Fibroblasts / Q000166:cytology*; D022001:Focal Adhesions; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D014945:Wound Healing