title: Serological and molecular detection of Borrelia burgdorferi sensu lato and Anaplasmataceae in rodents.
abstract: The presence of Anaplasma spp. and Borrelia burgdorferi sensu lato in rodents from Eastern Slovakia were followed by serological and molecular methods. The seroprevalence for Borrelia was detected in 16.6 %, for Anaplasmataceae (APT) in 13.2 % and co-occurrence of Borrelia and APT in 7.5 %. Out of 110 ear biopsies of rodents, 5 were B. afzelii-positive. Five biopsies tested positive with the Ehr521-Ehr747 primers amplifying all the members of the family APT. A. phagocytophilum was detected in 1.8 %, 2.7 % were infected with Anaplasma-like organisms. Co-occurrence of Borrelia and Anaplasma in ear biopsies was found in 1.8 %. The circulation of both Borrelia and Anaplasma in the region of Eastern Slovakia was confirmed.
authors.affiliation: Department of Natural Foci Diseases, Parasitological Institute, Slovak Academy of Sciences, Kosice, Slovakia. astefan@saske.sk
authors.forename: A
authors.initials: A
authors.lastname: Stefancíková
authors.forename: M
authors.initials: M
authors.lastname: Derdáková
authors.forename: D
authors.initials: D
authors.lastname: Lencáková
authors.forename: R
authors.initials: R
authors.lastname: Ivanová
authors.forename: M
authors.initials: M
authors.lastname: Stanko
authors.forename: L
authors.initials: L
authors.lastname: Cisláková
authors.forename: B
authors.initials: B
authors.lastname: Petko
mesh_terms: D000710:Anaplasmataceae / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D000711:Anaplasmataceae Infections / Q000175:diagnosis / Q000453:epidemiology / Q000382:microbiology / Q000662:veterinary*; D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D003411:Arvicolinae / Q000382:microbiology*; D015748:Borrelia burgdorferi Group / Q000302:isolation & purification*; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification; D004197:Disease Reservoirs; D004431:Ear, External / Q000382:microbiology; D004797:Enzyme-Linked Immunosorbent Assay; D008193:Lyme Disease / Q000175:diagnosis / Q000453:epidemiology / Q000382:microbiology / Q000662:veterinary*; D051379:Mice; D008969:Molecular Sequence Data; D051189:Murinae / Q000382:microbiology*; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D015203:Reproducibility of Results; D012376:Rodent Diseases / Q000175:diagnosis / Q000453:epidemiology / Q000382:microbiology*; D016415:Sequence Alignment; D016036:Seroepidemiologic Studies; D018154:Slovakia / Q000453:epidemiology
chemical_list: D000907:Antibodies, Bacterial; D004269:DNA, Bacterial