Protein phosphorylation goes negative.
Analyzing global protein phosphorylation by mass spectrometry remains a challenging task. To this end, Old et al. (2009) recently described a strategy that improves the coverage of phosphorylated sites and employed it to identify B-Raf-dependent phosphorylation sites in melanoma cells.
Pawson
Tony
T
Samuel Lunenfeld Research Institute, Mount Sinai Hospital, 600 University Avenue, Toronto, Ontario, Canada.
Taylor
Lorne
L
D045744:Cell Line, Tumor; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods; D008545:Melanoma / Q000378:metabolism*; D010766:Phosphorylation; D040901:Proteomics / Q000379:methods; D048493:Proto-Oncogene Proteins B-raf / Q000378:metabolism*; D015398:Signal Transduction
D048493:Proto-Oncogene Proteins B-raf