Detection of porcine rotavirus from tissue and faecal specimens.
Porcine small intestinal sub-mucosa is a cell-free collagen matrix that has demonstrated its ability as a scaffold material. Transplantation poses special hazards because grafted tissues and organs transmit pathogens efficiently, especially viruses. Rotavirus is thought to be confined to the intestine, causing acute diarrhoea. The purpose of this study was to evaluate the porcine intestinal tissue scaffold for Rotavirus and to study the incidence of this virus among pig herds. Only one isolate was successfully adapted to grow in cell line MA 104 from faecal samples. This isolate was further confirmed by reverse transcriptase polymerase chain reaction and sequence analysis.
Prabha
Suji
S
Department of Microbiology, International Centre for Cardio Thoracic and Vascular Diseases, Frontier Lifeline Pvt Ltd, Dr. K.M. Cherian Heart Foundation, Chennai, India. suji_prabha@sify.com
Verghese
Susan
S
D000818:Animals; D005243:Feces / Q000821:virology*; D007413:Intestinal Mucosa / Q000821:virology*; D008969:Molecular Sequence Data; D012367:RNA, Viral / Q000235:genetics; D012401:Rotavirus / Q000302:isolation & purification*; D012400:Rotavirus Infections / Q000662:veterinary* / Q000821:virology; D017422:Sequence Analysis, DNA; D013552:Swine; D013553:Swine Diseases / Q000821:virology*
D012367:RNA, Viral