title: Association of MICA with rheumatoid arthritis independent of known HLA-DRB1 risk alleles in a family-based and a case control study.
abstract: BACKGROUND
  The gene MICA encodes the protein major histocompatibility complex class I polypeptide-related sequence A. It is expressed in synovium of patients with rheumatoid arthritis (RA) and its implication in autoimmunity is discussed. We analyzed the association of genetic variants of MICA with susceptibility to RA.
  
  
  METHODS
  Initially, 300 French Caucasian individuals belonging to 100 RA trio families were studied. An additional 100 independent RA trio families and a German Caucasian case-control cohort (90/182 individuals) were available for replication. As MICA is situated in proximity to known risk alleles of the HLA-DRB1 locus, our analysis accounted for linkage disequilibrium either by analyzing the subgroup consisting of parents not carrying HLA-DRB1 risk alleles with transmission disequilibrium test (TDT) or by implementing a regression model including all available data. Analysis included a microsatellite polymorphism (GCT)n and single-nucleotide polymorphisms (SNPs) rs3763288 and rs1051794.
  
  
  RESULTS
  In contrast to the other investigated polymorphisms, the non-synonymously coding SNP MICA-250 (rs1051794, Lys196Glu) was strongly associated in the first family cohort (TDT: P = 0.014; regression model: odds ratio [OR] 0.46, 95% confidence interval [CI] 0.25 to 0.82, P = 0.007). Although the replication family sample showed only a trend, combined family data remained consistent with the hypothesis of MICA-250 association independent from shared epitope (SE) alleles (TDT: P = 0.027; regression model: OR 0.56, 95% CI 0.38 to 0.83, P = 0.003). We also replicated the protective association of MICA-250A within a German Caucasian cohort (OR 0.31, 95% CI 0.1 to 0.7, P = 0.005; regression model: OR 0.6, 95% CI 0.37 to 0.96, P = 0.032). We showed complete linkage disequilibrium of MICA-250 (D' = 1, r2= 1) with the functional MICA variant rs1051792 (D' = 1, r2= 1). As rs1051792 confers differential allelic affinity of MICA to the receptor NKG2D, this provides a possible functional explanation for the observed association.
  
  
  CONCLUSIONS
  We present evidence for linkage and association of MICA-250 (rs1051794) with RA independent of known HLA-DRB1 risk alleles, suggesting MICA as an RA susceptibility gene. However, more studies within other populations are necessary to prove the general relevance of this polymorphism for RA.
authors.affiliation: Center for Biotechnology and Biomedicine (BBZ), University of Leipzig, Deutscher Platz 5, 04103 Leipzig, Germany. holgerkirsten@web.de
authors.forename: Holger
authors.initials: H
authors.lastname: Kirsten
authors.forename: Elisabeth
authors.initials: E
authors.lastname: Petit-Teixeira
authors.forename: Markus
authors.initials: M
authors.lastname: Scholz
authors.forename: Dirk
authors.initials: D
authors.lastname: Hasenclever
authors.forename: Helene
authors.initials: H
authors.lastname: Hantmann
authors.forename: Dirk
authors.initials: D
authors.lastname: Heider
authors.forename: Ulf
authors.initials: U
authors.lastname: Wagner
authors.forename: Ulrich
authors.initials: U
authors.lastname: Sack
authors.forename: Vitor
authors.initials: V
authors.lastname: Hugo Teixeira
authors.forename: Bernard
authors.initials: B
authors.lastname: Prum
authors.forename: Jana
authors.initials: J
authors.lastname: Burkhardt
authors.forename: Céline
authors.initials: C
authors.lastname: Pierlot
authors.forename: Frank
authors.initials: F
authors.lastname: Emmrich
authors.forename: François
authors.initials: F
authors.lastname: Cornelis
authors.forename: Peter
authors.initials: P
authors.lastname: Ahnert
mesh_terms: D000328:Adult; D000483:Alleles; D001172:Arthritis, Rheumatoid / Q000235:genetics* / Q000276:immunology; D016022:Case-Control Studies; D015331:Cohort Studies; D005190:Family; D005260:Female; D008040:Genetic Linkage / Q000276:immunology; D020022:Genetic Predisposition to Disease / Q000235:genetics; D014644:Genetic Variation / Q000276:immunology; D006684:HLA-DR Antigens / Q000235:genetics*; D059811:HLA-DRB1 Chains; D015395:Histocompatibility Antigens Class I / Q000235:genetics*; D006801:Humans; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide / Q000235:genetics; D012307:Risk Factors
chemical_list: D006684:HLA-DR Antigens; D059811:HLA-DRB1 Chains; D015395:Histocompatibility Antigens Class I; C099187:MHC class I-related chain A