title: Analysis of human immunodeficiency virus type 1 nef gene sequences among inmates from prisons in China.
abstract: To provide the hard-won information on HIV-1 polymorphism from inmates in prison and to study the genetic variation of HIV-1 strains, we analyzed the characterization of the nucleotide and corresponding protein sequences of naturally occurring nef sequences derived from 16 HIV-1-positive inmates from prisons in China. Nested PCR and DNA sequencing were used to determine nef sequences in peripheral blood mononuclear cells. Phylogenetic analysis revealed a distribution of the viral sequences among subtype B, CRF_BC, and CRF_AE, with subtype B accounting for more than half of the genotypes. When compared with the consensus sequence, a certain amino acid sequence variability was observed. However, most of the described nef functional motifs were relatively well conserved in the majority of the sequences analyzed. Our results indicate that HIV-1 strains found in our study subjects may have a common origin and the description of motifs will produce important information for further studies of nef function.
authors.affiliation: Department of Epidemiology, Public Health College of Harbin Medical University, Harbin 150081, China.
authors.forename: Sheng-Yuan
authors.initials: SY
authors.lastname: Liu
authors.forename: Fang-Fang
authors.initials: FF
authors.lastname: Zeng
authors.forename: Guo-Feng
authors.initials: GF
authors.lastname: Huang
authors.forename: Hui
authors.initials: H
authors.lastname: Zhou
authors.forename: Yan-Mei
authors.initials: YM
authors.lastname: Shi
authors.forename: Hong
authors.initials: H
authors.lastname: Ling
authors.forename: Fu-Xiang
authors.initials: FX
authors.lastname: Wang
authors.forename: Bin-You
authors.initials: BY
authors.lastname: Wang
authors.forename: Jin
authors.initials: J
authors.lastname: Zhou
mesh_terms: D002681:China; D016000:Cluster Analysis; D005838:Genotype; D015658:HIV Infections / Q000821:virology*; D015497:HIV-1 / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000821:virology; D017720:Molecular Epidemiology; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D011329:Prisoners; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D054311:nef Gene Products, Human Immunodeficiency Virus / Q000235:genetics*
chemical_list: D054311:nef Gene Products, Human Immunodeficiency Virus; C514837:nef protein, Human immunodeficiency virus 1