High-throughput cellular microarray platforms: applications in drug discovery, toxicology and stem cell research.
Cellular microarrays are powerful experimental tools for high-throughput screening of large numbers of test samples. Miniaturization increases assay throughput while reducing reagent consumption and the number of cells required, making these systems attractive for a wide range of assays in drug discovery, toxicology, stem cell research and potentially therapy. Here, we provide an overview of the emerging technologies that can be used to generate cellular microarrays, and we highlight recent significant advances in the field. This emerging and multidisciplinary approach offers new opportunities for the design and control of stem cells in tissue engineering and cellular therapies and promises to expedite drug discovery in the biotechnology and pharmaceutical industries.
Fernandes
Tiago G
TG
Institute for Biotechnology and Bioengineering (IBB), Centre for Biological and Chemical Engineering, Instituto Superior Técnico, Av. Rovisco Pais, 1049-001 Lisboa, Portugal.
Diogo
Maria Margarida
MM
Clark
Douglas S
DS
Dordick
Jonathan S
JS
Cabral
Joaquim M S
JM
D000818:Animals; D002477:Cells; D055808:Drug Discovery / Q000379:methods*; D006801:Humans; D046210:Microfluidic Analytical Techniques; D020411:Oligonucleotide Array Sequence Analysis; D013234:Stem Cells; D046888:Tissue Array Analysis / Q000295:instrumentation / Q000379:methods / Q000639:trends; D018675:Toxicity Tests / Q000379:methods*