Total serum tryptase: a predictive marker for KIT mutation in acute myeloid leukemia.
Human tryptase is a serine protease expressed in mast-cells. We previously observed that AML blast cells, cultured in vitro from a KIT D816Y patient, give rise to adherent cells with mast-cell like phenotype and tryptase was released in the serum-free medium. To correlate total serum tryptase (ts-try) levels with cytogenetic features and KIT mutational status, we analyzed serum samples from AML patients at diagnosis. In 70 out of 155 patients (45%) we detected elevated ts-try (>15 ng/mL), significantly linked to t(8;21) (P < .001) and inv(16) (P = .007). In patients that achieved complete remission the ts-try decreased to normal values. In 75 patients screened for KIT mutation, we found a clear relationship between elevated ts-try and mutated patients with t(8;21) (P < .001). In conclusion, we propose that checking for ts-try at diagnosis of AML may be a simple tool to select patients to be addressed to KIT mutation screening.
Cairoli
Roberto
R
Department of Oncology, Niguarda Hospital, Piazza Ospedale Maggiore, 3-20162 Milan, Italy. roberto.cairoli@ospedaleniguarda.it
Ripamonti
Carla Barbara
CB
Beghini
Alessandro
A
Granata
Simonetta
S
Grillo
Giovanni
G
Brioschi
Matteo
M
Nadali
Gianpaolo
G
Viola
Assunta
A
Cattaneo
Chiara
C
Inropido
Liliana
L
Ravelli
Erika
E
Bertani
Giambattista
G
Pezzetti
Laura
L
Nichelatti
Michele
M
Marocchi
Alessandro
A
Rossi
Giuseppe
G
Pizzolo
Giovanni
G
Ferrara
Felicetto
F
Nosari
Anna Maria
AM
Morra
Enrica
E
D000293:Adolescent; D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000097:blood*; D016895:Culture Media, Serum-Free; D005260:Female; D006801:Humans; D015470:Leukemia, Myeloid, Acute / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D009154:Mutation; D019009:Proto-Oncogene Proteins c-kit / Q000235:genetics*; D055815:Young Adult
D014408:Biomarkers, Tumor; D016895:Culture Media, Serum-Free; D019009:Proto-Oncogene Proteins c-kit