title: A rapid microwave-assisted derivatization of bacterial metabolome samples for gas chromatography/mass spectrometry analysis.
abstract: Analysis of metabolome samples by gas chromatography/mass spectrometry requires a comprehensive derivatization method to afford quantitative and qualitative information of a complex biological sample. Here we describe an extremely time-effective microwave-assisted protocol for the commonly used methoxyamine and N-methyl-N-trimethylsilylfluoracetamide silylation method of primary metabolites. Our studies show that microwave irradiation can decrease the sample preparation time from approximately 120 min to 6 min without loss of either qualitative or quantitative information for the tested synthetic metabolite mixtures and microbial-derived metabolome samples collected from Bacillus subtilis and Staphylococcus aureus. Comparisons of metabolic fingerprints and selected metabolites show no noticeable differences compared with the commonly used heating block methods.
authors.affiliation: Institute of Pharmaceutical Biology, Ernst Moritz Arndt University of Greifswald, D-17487 Greifswald, Germany.
authors.forename: Manuel
authors.initials: M
authors.lastname: Liebeke
authors.forename: Ariane
authors.initials: A
authors.lastname: Wunder
authors.forename: Michael
authors.initials: M
authors.lastname: Lalk
mesh_terms: D001412:Bacillus subtilis / Q000378:metabolism*; D008401:Gas Chromatography-Mass Spectrometry / Q000191:economics / Q000379:methods*; D055442:Metabolome; D055432:Metabolomics / Q000191:economics / Q000379:methods*; D008872:Microwaves; D013211:Staphylococcus aureus / Q000378:metabolism*; D013997:Time Factors