Proteomic analysis of the development and germination of date palm (Phoenix dactylifera L.) zygotic embryos.
By using a comparative proteomic approach (2-DE coupled to MS/MS), the development, maturation, and germination of date palm zygotic embryos, have been studied. Proteins were trichloroacetic acid (TCA)-acetone-phenol extracted and resolved by 2-DE in the 5-8 pH range. The total protein content and the number of spots resolved increased from early (12 weeks after pollination (WAP); 68.96 mg/g DW: 207 spots) to late (17 WAP; 240.85 mg/g DW: 261 spots) stages, decreasing upon germination (from 120.8 mg/g DW: 273 spots in mature embryos to 26.35 mg/g DW: 87 spots in 15 days after germination). Up to 194 spots showed qualitative or quantitative differences between stages. Statistical analysis of spot variation was performed by PCA, obtaining a more accurate grouping of the samples and determining the most discriminant spots. Samples were also clustered based on Pearson distance and Ward's minimum distance. Sixty-five variable spots were subjected to MS analysis, resulting in 21 identifications. The identified proteins belong to the following functional categories: enzymes of glycolysis, tricarboxylic acid cycle, and carbohydrate biosynthesis, protein translation, storage (glutelin), and stress-related proteins. The evolution pattern of the functional groups was examined and discussed in terms of metabolism adaptation to the different embryogenic and germination stages.
Sghaier-Hammami
Besma
B
Laboratoire des Biotechnologies Végétales Appliquées à l'Amélioration des Cultures, Faculté des Sciences de Sfax, Sfax, Tunisie.
Valledor
Luis
L
Drira
Noureddine
N
Jorrin-Novo
Jesús V
JV
D000704:Analysis of Variance; D028023:Arecaceae / Q000196:embryology / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D016000:Cluster Analysis; D015180:Electrophoresis, Gel, Two-Dimensional; D018506:Gene Expression Regulation, Plant; D018525:Germination / Q000502:physiology*; D010940:Plant Proteins / Q000737:chemistry / Q000378:metabolism*; D025341:Principal Component Analysis; D012639:Seeds / Q000196:embryology / Q000254:growth & development / Q000378:metabolism*; D018709:Statistics, Nonparametric; D053719:Tandem Mass Spectrometry
D010940:Plant Proteins