Donor age-dependent acceleration of cellular aging by  repeated ultraviolet A irradiation of human dermal  fibroblasts derived from a single donor.
The relationship between cellular aging and aging of entire organisms has been studied extensively.The findings are confusing, however, and no clear relationships have been demonstrated.The conflicting data may be due to individual differences among the donors of the studied cells.It is crucial to identify the changes in cellular properties that are the result of the aging process.Here, we used human dermal fibroblast cell lines established from a single donor at different ages to assess the influence of ultraviolet A (UVA) on cellular aging. These cell lines have the same genetic background and were obtained from a restricted body region. The results indicated that cellular aging was accelerated by UVA irradiation in a donor age-dependent manner. The ratio of lifespan shortening increased with donor age. Increased donor age not only decreased cell division, but also increased the growth arrest response to UVA irradiation. The characteristics of the cultured cells reflected the age-related changes in dermal fibroblasts.
Naru
Eiji
E
Research and Development Division, KOSE Corporation, 48-18 Sakae-cho, Kita-ku, Tokyo, Japan. e-naru@kose.co.jp
Ohta
Toshiro
T
Inomata
Ken
K
Hayashi
Akinobu
A
Kaji
Kazuhiko
K
D000328:Adult; D000375:Aging / Q000502:physiology*; D002453:Cell Cycle / Q000528:radiation effects; D002460:Cell Line; D016922:Cellular Senescence / Q000528:radiation effects*; D020405:Dermis / Q000166:cytology*; D004307:Dose-Response Relationship, Radiation; D005347:Fibroblasts / Q000473:pathology / Q000528:radiation effects*; D006801:Humans; D008875:Middle Aged; D014466:Ultraviolet Rays