In situ microextraction method to determine the viscosity of biofluid in threadlike structures on the surfaces of mammalian organs.
We report a method to measure the viscosity of microL volumes of biofluid obtained from threadlike structures (NTSs) on the surfaces of mammalian (rabbit) internal organs. The fluid was mechanically microextracted in situ from NTSs on the organ surfaces by a glass capillary connected to an extractor. From the Brownian motion of the 0.8+/-0.1microm diameter granules in the extracted fluid, the fluid viscosity was determined to be 1.4+/-0.1mPa s at room temperature. This viscosity is comparable to the viscosity of rabbit blood plasma.
Sung
Baeckkyoung
B
Biomedical Physics Laboratory, Department of Physics and Astronomy, Seoul National University, Seoul 151-747, Korea.
Kim
Min Su
MS
Corrigan
Adam
A
Donald
Athene M
AM
Soh
Kwang-Sup
KS
D000818:Animals; D001826:Body Fluids / Q000737:chemistry*; D009391:Nephelometry and Turbidimetry / Q000379:methods*; D011817:Rabbits; D052617:Solid Phase Microextraction / Q000379:methods*; D013499:Surface Properties; D014781:Viscera / Q000502:physiology*; D014783:Viscosity