TRBP homolog interacts with eukaryotic initiation factor 6 (eIF6) in Fenneropenaeus chinensis.
The HIV transactivating response RNA-binding protein (TRBP) plays an important role in many biological processes. We have cloned three cDNAs from newly identified genes in the TRBP family from Fenneropenaeus chinensis. These genes have been designated Fc-TRBP1-3. Recombinant Fc-TRBP1, which was produced in Escherichia coli, was used for panning of a T7 phage display library of the Chinese shrimp hemocytes. From this panning, Fc-eukaryotic initiation factor 6 (Fc-eIF6) was isolated and sequenced. Fc-eIF6 was then cloned, recombinantly expressed, and shown to interact with Fc-TRBP by the performance of pull-down assays and Far Western blot analysis. Expression of Fc-TRBP was detected in many tissues, with elevated expression in the heart, gill, and intestine in the early stages of infection by the white spot syndrome virus (WSSV), and enhanced expression in most tissues following challenge with Vibrio anguillarum. Western blot studies confirmed the increased expression of Fc-TRBP in the gill after WSSV infection. The expression pattern of eIF6 was also analyzed and its expression was also up-regulated in intestine of WSSV-challenged shrimp. The replication of WSSV was reduced after injection of Fc-TRBP. These results indicate that Fc-TRBP and Fc-eIF6 may be components of the RNA-induced silencing complex (RISC), and thereby play a crucial role in the antiviral defense response of shrimp.
Wang
Shuai
S
School of Life Sciences, Shandong University, and The Key Laboratory of Plant Cell Engineering and Germplasm Innovation of Ministry of Education, Jinan, Shandong, China.
Liu
Ning
N
Chen
An-Jing
AJ
Zhao
Xiao-Fan
XF
Wang
Jin-Xing
JX
D000595:Amino Acid Sequence; D000818:Animals; D017123:Bacteriophage T7 / Q000737:chemistry / Q000378:metabolism; D008969:Molecular Sequence Data; D033561:Penaeidae / Q000737:chemistry* / Q000276:immunology* / Q000821:virology; D010448:Peptide Initiation Factors / Q000096:biosynthesis / Q000302:isolation & purification / Q000378:metabolism*; D019151:Peptide Library; D020033:Protein Isoforms / Q000096:biosynthesis / Q000302:isolation & purification / Q000378:metabolism; D016601:RNA-Binding Proteins / Q000096:biosynthesis / Q000302:isolation & purification / Q000378:metabolism*; D017386:Sequence Homology, Amino Acid; D014733:Vibrio / Q000276:immunology / Q000378:metabolism; D000099253:Trans-Activation Responsive RNA-Binding Protein
D010448:Peptide Initiation Factors; D019151:Peptide Library; D020033:Protein Isoforms; D016601:RNA-Binding Proteins; D000099253:Trans-Activation Responsive RNA-Binding Protein; C021019:eIF-6