Methicillin-resistant Staphylococcus aureus among a network of French private-sector community-based-medical laboratories.
OBJECTIVE
The aim of this study was to estimate the frequency of methicillin-resistant Staphylococcus aureus (MRSA) strains in the French community and the proportion of Panton-Valentine (PVL)-MRSA.


METHODS
A cross-sectional study was made during a 3-month period in 2003 through a network of private-sector, community-based medical laboratories selected throughout France: the Labville network. Each MRSA isolate was included and characterized by French National Reference Center for Staphylococci. The total number of S. aureus isolates was also collected.


RESULTS
Among the 283 patients infected or colonized by MRSA, 166 (59%) were considered as healthcare-associated, 14 (5%) as nursing-associated and 39 (14%) as community-acquired. The proportion of methicillin resistance among S. aureus was 14%. Taking into account the sampling design, the incidence of MRSA cases in French outpatients was estimated to be 0.50 [CI95%: 0.41-0.60] per 10,000 inhabitants. The molecular analysis confirmed that 80.6% belong to the Lyon clone, the most prevalent hospital MRSA clone spreading in France and 10.6% to a closely related clone. An emerging MRSA clone containing the tst1 gene was detected in six patients and the PVL-positive ST80 clone only in one, 22-year-old, patient.


CONCLUSIONS
Most of MRSA cases diagnosed in the community in France, in 2003, were elderly with specific risk factors and harbored hospital strains. The prevalence of PVL-MRSA remained low.
Maugat
S
S
Département maladies infectieuses, institut de veille sanitaire, 12, rue du Val-d'Osne, 94415 Saint-Maurice cedex, France. s.maugat@invs.sante.fr
de Rougemont
A
A
Aubry-Damon
H
H
Reverdy
M-E
ME
Georges
S
S
Vandenesch
F
F
Etienne
J
J
Coignard
B
B
D001769:Blood / Q000382:microbiology; D017714:Community-Acquired Infections / Q000453:epidemiology* / Q000635:transmission; D005243:Feces / Q000382:microbiology; D005260:Female; D005602:France / Q000453:epidemiology; D006801:Humans; D015994:Incidence; D007753:Laboratories / Q000592:standards*; D008297:Male; D055624:Methicillin-Resistant Staphylococcus aureus / Q000302:isolation & purification; D010045:Outpatients; D012744:Sex Ratio; D013203:Staphylococcal Infections / Q000453:epidemiology* / Q000635:transmission