A novel single-cell DNA fingerprinting method successfully distinguishes sibling human embryos.
OBJECTIVE
To validate a novel system for embryonic DNA fingerprinting which can reliably distinguish sibling embryos from each other.


METHODS
Prospective, randomized, and blinded study.


METHODS
Academic center for reproductive medicine.


METHODS
Blastomeres were obtained from discarded and transferred embryos from six patients undergoing IVF treatment.


METHODS
None.


METHODS
Single lymphocytes from sibling cell lines and blastomeres from sibling day 3 human embryos were evaluated for accurate assignment of relationship using whole genome amplification and single-nucleotide polymorphism microarrays.


RESULTS
Assignment of single-cell relationships was accomplished with 100% accuracy. We also observed complete agreement between the molecular karyotype and DNA fingerprint-based identification of embryos implanted in three clinical IVF cases after multiple embryo transfer.


CONCLUSIONS
These data demonstrate the first single-blastomere DNA fingerprinting technology capable of unequivocal discrimination of sibling human embryos.
Treff
Nathan R
NR
Reproductive Medicine Associates of New Jersey, Morristown, New Jersey 07960, USA. ntreff@rmanj.com
Su
Jing
J
Tao
Xin
X
Miller
Kathleen A
KA
Levy
Brynn
B
Scott
Richard T
RT
D001402:B-Lymphocytes / Q000502:physiology; D001757:Blastomeres / Q000166:cytology* / Q000502:physiology*; D015193:Chorionic Villi Sampling; D016172:DNA Fingerprinting / Q000379:methods*; D004624:Embryo Transfer; D005260:Female; D005307:Fertilization in Vitro; D055106:Genome-Wide Association Study; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis; D020641:Polymorphism, Single Nucleotide; D011247:Pregnancy; D011446:Prospective Studies; D035781:Siblings