title: Intense pulsed light effects on the expression of extracellular matrix proteins and transforming growth factor beta-1 in skin dermal fibroblasts cultured within contracted collagen lattices.
abstract: BACKGROUND
  Emerging clinical evidence suggests that intense pulsed light (IPL) treatment may exert some beneficial effects on photoaged skin. The molecular mechanisms underlying this IPL effect have not been fully elucidated.
  
  
  OBJECTIVE
  To examine the effects of IPL irradiation on normal human dermal fibroblasts grown in contracted collagen lattices.
  
  
  METHODS
  Human skin fibroblasts cultured in contracted collagen lattices were irradiated with IPL with triple pulses of 7 ms with a pulse interval of 70 ms and fluences of 20, 50, and 75 J/cm(2). Twenty-four hours after the irradiation, cell viability, messenger RNA (mRNA), and protein levels of extracellular matrix proteins (e.g., collagen I, collagen III, and fibronectin) and transforming growth factor beta-1 (TGF-beta1) were evaluated using dye exclusion, real-time reverse transcriptase polymerase chain reaction, and enzyme-linked immunosorbent assay, respectively.
  
  
  RESULTS
  A dose-dependent increase in viable cells was demonstrated after the IPL irradiation. There was no significant change in mRNA levels of collagen I and fibronectin. Upregulated expression of collagen III and TGF-beta1 in dermal fibroblasts was verified.
  
  
  CONCLUSIONS
  The analytical results presented here provide a potential mechanistic explanation for the mechanism of clinical photorejuvenation effects of IPL that involves the increase of extracellular matrix construction by upregulating the gene expressions of collagen III and TGF-beta1.
authors.affiliation: Graduate Institute of Clinical Medical Sciences, Chang Gung Memorial Hospital, Taipei, Taiwan.
authors.forename: Wen-Rou
authors.initials: WR
authors.lastname: Wong
authors.forename: Wen-Ling
authors.initials: WL
authors.lastname: Shyu
authors.forename: Jung-Wei
authors.initials: JW
authors.lastname: Tsai
authors.forename: Kuang-Hung
authors.initials: KH
authors.lastname: Hsu
authors.forename: Jong-Hwei S
authors.initials: JH
authors.lastname: Pang
mesh_terms: D002478:Cells, Cultured; D024061:Collagen Type III / Q000096:biosynthesis / Q000235:genetics*; D017077:Culture Media, Conditioned; D020405:Dermis / Q000166:cytology / Q000378:metabolism* / Q000528:radiation effects; D004797:Enzyme-Linked Immunosorbent Assay; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism* / Q000528:radiation effects; D005786:Gene Expression Regulation / Q000528:radiation effects*; D006801:Humans; D010789:Phototherapy / Q000379:methods*; D012313:RNA / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D053773:Transforming Growth Factor beta1 / Q000096:biosynthesis / Q000235:genetics*
chemical_list: D024061:Collagen Type III; D017077:Culture Media, Conditioned; D053773:Transforming Growth Factor beta1; D012313:RNA