[Studies on extraction of Ligusticum chuanxiong in Hongye Xintong soft capsule by super critical fluid extraction].
OBJECTIVE
To study the rationality of extraction prosess for the Ligusticum chuanxiong in Hongye Xintong Soft Capsule by super critical fluid extraction(SFE).


METHODS
Conditions for the extraction were optimized by orthogonal experimental design as guided by the extraction rate and content of ligustilid in the extract; Combined with the experiment of rats ligated the left coronary artery, the two compounds containing different extracts (steam distillation and SFE) were compared to determine the extraction prosess.


RESULTS
The best extraction conditions were established as following: pressure 30 MPa, temperature 50 degrees C, extracting time 4 h, separate pressure 10 MPa. separate temperature 40 degrees C. Compared with steam distillation, the compound containing SFE extract showed stronger protective effects on rats ligated the left coronary artery.


CONCLUSIONS
Super critical fluid can extract active ingredients in Ligusticum chuanxiong effectively.
Lan
Yanyu
Y
School of Pharmacy, Guiyang Medical College, Guiyang 550004, China.
Zheng
Lin
L
Huang
Yong
Y
Wang
Aimin
A
Li
Yongjun
Y
He
Xun
X
Wang
Yonglin
Y
D000818:Animals; D002214:Capsules; D025924:Chromatography, Supercritical Fluid / Q000379:methods*; D003331:Coronary Vessels / Q000187:drug effects; D004365:Drugs, Chinese Herbal / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D005260:Female; D030004:Ligusticum; D008297:Male; D011312:Pressure; D051381:Rats; D013696:Temperature; D013997:Time Factors; D014867:Water / Q000737:chemistry
D002214:Capsules; D004365:Drugs, Chinese Herbal; C540809:hongye xintong; D014867:Water; C101574:chuangxiong extract