Evaluation of the contribution of the renal capsule and cortex to kidney autofluorescence intensity under ultraviolet excitation.
The use of reduced nicotinamide adenine dinucleotide (NADH) fluorescence to gain metabolic information on kidneys in response to an alteration in oxygen availability has previously been experimentally demonstrated, but signal quantification has not, to date, been addressed. In this work the relative contribution to rat kidney autofluorescence of the capsule versus cortex under ultraviolet excitation is determined from experimental results obtained using autofluorescence microscopy and a suitable mathematical model. The results allow for a quantitative assessment of the relative contribution of the signal originating in the metabolically active cortex as a function of capsule thickness for different wavelengths.
Raman
Rajesh N
RN
Pivetti
Christopher D
CD
Rubenchik
Alexander M
AM
Matthews
Dennis L
DL
Troppmann
Christoph
C
Demos
Stavros G
SG
D000465:Algorithms; D000818:Animals; D050476:Bowman Capsule / Q000378:metabolism*; D066298:In Vitro Techniques; D007672:Kidney Cortex / Q000378:metabolism*; D009243:NAD / Q000032:analysis*; D051381:Rats; D011917:Rats, Inbred Lew; D011920:Rats, Inbred WF; D017207:Rats, Sprague-Dawley; D013050:Spectrometry, Fluorescence / Q000379:methods*; D014018:Tissue Distribution; D014466:Ultraviolet Rays
D009243:NAD