Effect of pro-inflammatory cytokine stimulation on human breast cancer: implications of chemokine receptor expression in cancer metastasis.
Interactions between tumour cells and microenvironments may affect their growth and metastasis formation. In search for a better understanding of the role of cellular mediators in the progression of cancer, we investigated the effect of pro-inflammatory cytokines IL-1, IL-6, TNF-alpha and IFN-gamma on the regulation of expression of chemokine receptors CXCR4, CXCR2, CX3CR1, CCR9, and CCR5 in the human breast cancer cell line MCF-7. Our results showed that IL-1 increased CXCR4 expression whereas TNF-alpha increased CX3CR1, CCR9 and CCR5. Interestingly, this regulation was not homogeneous, emphasizing the inherent heterogeneity in cancer that may be responsive to specific inflammatory microenvironments.
Valdivia-Silva
Julio E
JE
Departamento de Inmunología, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, Ciudad Universitaria, Circuito exterior s/n, C.P. 04510 DF, Mexico.
Franco-Barraza
Janusz
J
Silva
Ana Luisa Esparza
AL
Pont
Gisela Du
GD
Soldevila
Gloria
G
Meza
Isaura
I
García-Zepeda
Eduardo A
EA
D001943:Breast Neoplasms / Q000276:immunology* / Q000473:pathology; D045744:Cell Line, Tumor; D002633:Chemotaxis / Q000276:immunology; D016207:Cytokines / Q000276:immunology*; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D009362:Neoplasm Metastasis / Q000276:immunology*; D012333:RNA, Messenger / Q000032:analysis; D019707:Receptors, Chemokine / Q000096:biosynthesis*; D020133:Reverse Transcriptase Polymerase Chain Reaction
D016207:Cytokines; D012333:RNA, Messenger; D019707:Receptors, Chemokine