title: The epithelial-mesenchymal transition induced by keratinocyte growth conditions is overcome by E6 and E7 from HPV16, but not HPV8 and HPV38: characterization of global transcription profiles.
abstract: The aim of this study was to evaluate the growth properties of primary human keratinocytes expressing E6 and E7 proteins, which are from either the beta- or alpha-genotypes, under different culture conditions. We demonstrated that keratinocytes expressing E6 and E7, from both HPV8 and 38, irreversibly underwent the epithelial-mesenchymal transition (EMT) when grown on plastic with FAD medium (F12/DMEM/5%FBS). Expression of E6/E7 from HPV16 was capable of fully overcoming the FAD-induced EMT. Immortalization was only observed in HPV16-transduced cell lines, while the more proliferating phenotype of both KerHPV8 and 38 was mainly related to FAD-induced EMT. Microarray analysis of exponentially growing cells identified 146 cellular genes that were differentially regulated in HPV16 compared to HPV8- and 38-transduced cells. A large accumulation of transcripts associated with epidermal development and differentiation was observed in HPV16-transduced cells, whereas transcripts of genes involved in the extracellular matrix, multicellular organismal processes, and inflammatory response were affected in HPV8 and 38-transduced cells.
authors.affiliation: Department of Clinical and Experimental Medicine, Medical School of Novara, via Solaroli 17, 28100 Novara, Italy.
authors.forename: Barbara
authors.initials: B
authors.lastname: Azzimonti
authors.forename: Valentina
authors.initials: V
authors.lastname: Dell'oste
authors.forename: Cinzia
authors.initials: C
authors.lastname: Borgogna
authors.forename: Michele
authors.initials: M
authors.lastname: Mondini
authors.forename: Francesca
authors.initials: F
authors.lastname: Gugliesi
authors.forename: Marco
authors.initials: M
authors.lastname: De Andrea
authors.forename: Giovanna
authors.initials: G
authors.lastname: Chiorino
authors.forename: Maria
authors.initials: M
authors.lastname: Scatolini
authors.forename: Chiara
authors.initials: C
authors.lastname: Ghimenti
authors.forename: Santo
authors.initials: S
authors.lastname: Landolfo
authors.forename: Marisa
authors.initials: M
authors.lastname: Gariglio
mesh_terms: D002454:Cell Differentiation / Q000235:genetics; D002472:Cell Transformation, Viral / Q000235:genetics; D002478:Cells, Cultured / Q000378:metabolism; D004847:Epithelial Cells / Q000378:metabolism / Q000821:virology; D020869:Gene Expression Profiling; D015967:Gene Expression Regulation, Viral; D005822:Genetic Vectors / Q000378:metabolism; D052162:Human papillomavirus 16 / Q000378:metabolism*; D006801:Humans; D015603:Keratinocytes / Q000166:cytology / Q000378:metabolism / Q000821:virology*; D008648:Mesoderm / Q000166:cytology; D009856:Oncogene Proteins, Viral / Q000235:genetics / Q000378:metabolism*; D009924:Organ Culture Techniques; D027383:Papillomaviridae / Q000235:genetics / Q000378:metabolism*; D050725:Papillomavirus E7 Proteins / Q000235:genetics / Q000378:metabolism*; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: C068651:E6 protein, Human papillomavirus type 16; D009856:Oncogene Proteins, Viral; D050725:Papillomavirus E7 Proteins; D012097:Repressor Proteins