Effect of serum and growth factors on chondrogenic differentiation of synovium-derived stromal cells.
We used fetal bovine serum (FBS) and different growth factors to investigate their potential for inducing chondrogenic differentiation of synovium-derived stromal cells. Human synovium was harvested from patients suffering from osteoarthritis and expanded in monolayer. To evaluate the effect of serum and growth factors on chondrogenic differentiation, 10 ng/mL of transforming growth factor-beta1 (TGF-beta1), 100 ng/mL of bone morphogenic protein-2 (BMP-2), 100 ng/mL of insulin-like growth factor-1, 20 ng/mL of basic fibroblast growth factor (bFGF), and 10% FBS were added to the chemically defined chondrogenic medium singly or in combination during pellet culture for 21 days. The cell size and weight, glycosaminoglycan content, histology, and cartilage matrix-associated genes expression were analyzed. TGF-beta1 alone and TGF-beta1 + BMP-2 induced chondrogenic differentiation of synovium-derived stromal cells and synthesized cartilage-like matrix confirmed by histological analysis and immunohistochemistry. FBS, BMP-2, insulin-like growth factor-1, and bFGF as a single factor or other combinations except for TGF-beta1 + BMP-2 hardly induced chondrogenesis. Chondrogenic differentiation appeared to be inhibited when bFGF or the serum was added to the chondrogenic medium during pellet culture. The results of this study demonstrate the negative or positive role of serum and growth factors on chondrogenic differentiation of synovium-derived stromal cells.
Lee
Sahnghoon
S
Department of Orthopaedic Surgery, Seoul National University College of Medicine, Seoul, Korea.
Kim
Ji Hyun
JH
Jo
Chris Hyunchul
CH
Seong
Sang Cheol
SC
Lee
Jae Chul
JC
Lee
Myung Chul
MC
D000368:Aged; D000369:Aged, 80 and over; D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D019902:Chondrocytes / Q000166:cytology* / Q000187:drug effects / Q000502:physiology; D020219:Chondrogenesis / Q000187:drug effects / Q000502:physiology*; D016207:Cytokines / Q000378:metabolism; D005260:Female; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins / Q000494:pharmacology*; D008297:Male; D008875:Middle Aged; D044967:Serum / Q000378:metabolism*; D017154:Stromal Cells / Q000166:cytology* / Q000187:drug effects / Q000502:physiology; D013583:Synovial Membrane / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism*; D023822:Tissue Engineering / Q000379:methods
D016207:Cytokines; D036341:Intercellular Signaling Peptides and Proteins