title: Thrombospondin 1 expression and angiogenesis in breast carcinoma and their relation with platelet activity.
abstract: This study investigates angiogenesis and the expression of thrombospondin 1 in invasive ductal carcinoma of the breast and their possible relation to platelet counts and platelet activity. The study included 20 cases of invasive ductal carcinoma. Platelet activity was evaluated by determining thromboxane B2 and cyclic guanosine monophosphate (cGMP) levels by enzyme immunoassay (EIA).Thrombospondin (TSP) 1 and CD34 expression was studied immunohistochemically. Mean platelet count of the patient group was significantly greater than the mean platelet count of the control group (P < 0.05). The platelet counts were positively correlated with tumour size (r=0.609; P < 0.01). Platelet counts were higher in the patients who had grade 3 microvessel density (P < 0.05). The mean basal platelet cGMP level in the patient group was significantly lower than it was in the control group (P < 0.05). Focal TSP immunoreactivity was detectable in 5 (20%) cases in the tumour cells, and in 9 (45%) cases in the stroma. We did not find any correlation between TSP-1 staining and angiogenesis, platelet counts, platelet activity, and the histological prognostic parameters. Our study confirms the essential role of platelets in tumour growth and angiogenesis. Decreased levels of cGMP in the patient group may cause platelet hyperreactivity. Although thrombospondin 1 may be upregulated in malignant breast tissue, this is not sufficient for tumour growth and dissemination according to our results.
authors.affiliation: Department of Pathology, School of Medicine, Afyon Kocatepe University, Afyonkarahisar, Turkey. ctokyol@yahoo.com
authors.forename: Cigdem
authors.initials: C
authors.lastname: Tokyol
authors.forename: Gulriz
authors.initials: G
authors.lastname: Ersoz
authors.forename: Fatma Husniye
authors.initials: FH
authors.lastname: Dilek
authors.forename: Ercan
authors.initials: E
authors.lastname: Gencer
authors.forename: Mehmet Nuri
authors.initials: MN
authors.lastname: Kosar
authors.forename: Osman Nuri
authors.initials: ON
authors.lastname: Dilek
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001792:Blood Platelets / Q000166:cytology*; D001943:Breast Neoplasms / Q000097:blood / Q000378:metabolism* / Q000503:physiopathology; D016022:Case-Control Studies; D006152:Cyclic GMP / Q000378:metabolism; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D007150:Immunohistochemistry; D008875:Middle Aged; D009389:Neovascularization, Pathologic; D019700:Thrombospondin 1 / Q000378:metabolism*
chemical_list: D019700:Thrombospondin 1; D006152:Cyclic GMP