title: Generation of an ABCG2(GFPn-puro) transgenic line--a tool to study ABCG2 expression in mice.
abstract: The ATP-binding cassette (ABC) transporter 2 (ABCG2) is expressed by stem cells in many organs and in stem cells of solid tumors. These cells are isolated based on the side population (SP) phenotype, a Hoechst 3342 dye efflux property believed to be conferred by ABCG2. Because of the limitations of this approach we generated transgenic mice that express Nuclear GFP (GFPn) coupled to the Puromycin-resistance gene, under the control of ABCG2 promoter/enhancer sequences. We show that ABCG2 is expressed in neural progenitors of the developing forebrain and spinal cord and in embryonic and adult endothelial cells of the brain. Using the neurosphere assay, we isolated tripotent ABCG2-expressing neural stem cells from embryonic mouse brain. This transgenic line is a powerful tool for studying the expression of ABCG2 in many tissues and for performing functional studies in different experimental settings.
authors.affiliation: The Cyprus Institute of Neurology and Genetics, Airport Avenue, No. 6, Agios Dometios 2370, Nicosia, Cyprus.
authors.forename: Michael
authors.initials: M
authors.lastname: Orford
authors.forename: Richard
authors.initials: R
authors.lastname: Mean
authors.forename: George
authors.initials: G
authors.lastname: Lapathitis
authors.forename: Nicholas
authors.initials: N
authors.lastname: Genethliou
authors.forename: Elena
authors.initials: E
authors.lastname: Panayiotou
authors.forename: Helen
authors.initials: H
authors.lastname: Panayi
authors.forename: Stavros
authors.initials: S
authors.lastname: Malas
mesh_terms: D000070996:ATP Binding Cassette Transporter, Subfamily G; D018528:ATP-Binding Cassette Transporters / Q000096:biosynthesis* / Q000235:genetics; D000483:Alleles; D000818:Animals; D000964:Antimetabolites, Antineoplastic / Q000494:pharmacology; D001921:Brain / Q000166:cytology / Q000196:embryology / Q000378:metabolism*; D002467:Cell Nucleus / Q000378:metabolism; D019008:Drug Resistance, Neoplasm / Q000235:genetics; D004742:Enhancer Elements, Genetic; D049452:Green Fluorescent Proteins / Q000096:biosynthesis / Q000235:genetics; D051379:Mice; D008822:Mice, Transgenic; D011401:Promoter Regions, Genetic; D011691:Puromycin / Q000494:pharmacology
chemical_list: D000070996:ATP Binding Cassette Transporter, Subfamily G; D018528:ATP-Binding Cassette Transporters; C420604:Abcg3 protein, mouse; D000964:Antimetabolites, Antineoplastic; D049452:Green Fluorescent Proteins; D011691:Puromycin