title: Hairy1 acts as a node downstream of Wnt signaling to maintain retinal stem cell-like progenitor cells in the chick ciliary marginal zone.
abstract: In the vertebrate retina, stem cell-like progenitor cells are maintained in a distinct region called the ciliary marginal zone (CMZ). Canonical Wnt signaling regulates the maintenance of the progenitor cells in the CMZ. However, its downstream molecular mechanisms have remained largely unclear. Here, we show that chick Hairy1, an established Notch signaling effector, mediates the Wnt-dependent maintenance of CMZ progenitor cells in chicken. Interestingly, unlike other developmental contexts in which Hes gene expression is regulated by Notch signaling, Hairy1 expression in the CMZ is regulated by Wnt signaling. Hairy1 is necessary and sufficient for the expression of a set of molecular markers characteristic of the CMZ, and Wnt2b fails to induce CMZ markers when Hairy1 activity is inhibited. Furthermore, microarray analysis identifies multiple Wnt-responsive transcription factors that activate Hairy1 expression. We thus propose that Hairy1 functions as a node downstream of Wnt signaling to maintain progenitor cells in the chick CMZ.
authors.affiliation: RIKEN Advanced Science Institute, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan.
authors.forename: Fumi
authors.initials: F
authors.lastname: Kubo
authors.forename: Shinichi
authors.initials: S
authors.lastname: Nakagawa
mesh_terms: D000818:Animals; D030161:Avian Proteins / Q000235:genetics / Q000502:physiology*; D051792:Basic Helix-Loop-Helix Proteins / Q000235:genetics / Q000502:physiology*; D002454:Cell Differentiation; D002642:Chick Embryo; D020411:Oligonucleotide Array Sequence Analysis; D051880:Receptors, Notch / Q000502:physiology; D012160:Retina / Q000166:cytology* / Q000196:embryology / Q000502:physiology; D013234:Stem Cells / Q000166:cytology* / Q000502:physiology; D051153:Wnt Proteins / Q000502:physiology*
chemical_list: D030161:Avian Proteins; D051792:Basic Helix-Loop-Helix Proteins; D051880:Receptors, Notch; D051153:Wnt Proteins; C109424:hairy1 protein, Gallus gallus