title: Patterning of HeLa cells on a microfabricated Au-coated ITO substrate.
abstract: HeLa cells were patterned on a microfabricated Au-coated ITO substrate. Part of the Au film was dissolved by the electrochemical wet stamping technique, and the substrate was then immersed in a thiol-terminated methoxy-poly(ethylene glycol) (mPEG-SH) solution for further modification. Self-assembled monolayers (SAMs) on the gold island can form cell- and protein-resistant regions on the substrate, and living HeLa cells can adhere to the exposed ITO surface. Single cell patterns could be achieved when the size of a microstructure unit matched that of the cell. The fluorescence experiments further proved that living HeLa cells prefer to attach to the ITO substrate rather than the mPEG-SH-modified gold islands.
authors.affiliation: Department of Chemistry, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, 361005, China.
authors.forename: Li H
authors.initials: LH
authors.lastname: Jin
authors.forename: Bing Y
authors.initials: BY
authors.lastname: Yang
authors.forename: Li
authors.initials: L
authors.lastname: Zhang
authors.forename: Pei L
authors.initials: PL
authors.lastname: Lin
authors.forename: Chen
authors.initials: C
authors.lastname: Cui
authors.forename: Jing
authors.initials: J
authors.lastname: Tang
mesh_terms: D002448:Cell Adhesion; D018914:Cells, Immobilized / Q000166:cytology / Q000378:metabolism*; D006046:Gold / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D007204:Indium / Q000737:chemistry*; D013499:Surface Properties; D017971:Tin Compounds / Q000737:chemistry*
chemical_list: D017971:Tin Compounds; D007204:Indium; D006046:Gold; C045358:stannic oxide