title: Adeno-associated virus and human papillomavirus types in cervical samples of pregnant and non-pregnant women.
abstract: OBJECTIVE
  To investigate the prevalence of AAV and HPV DNA and their types in cervical secretion from pregnant and non-pregnant women.
  
  
  METHODS
  The samples were obtained from 40 pregnant and 62 non-pregnant women who were attended at the outpatient clinic of the Federal University Hospital of Espírito Santo, Southeastern Brazil. AAV and HPV were investigated by PCR and typed by PCR and/or RFLP.
  
  
  RESULTS
  The occurrence of AAV in all samples was 25.5% (26/102): 81% (21/26) and 19% (5/26) for AAV2/3 and AAV5 species, respectively. AAV were observed in 35% (14/40) and 19% (12/62) of pregnant and non-pregnant women, respectively. HPV occurred in 22% of all samples; 25% (10/40) in pregnant and 20% (12/60) in non-pregnant women. HPV types were determined for 72.7% of the strains, most of which classified as high-risk. AAV-HPV co-infection was observed in 15.4% (4/26), mostly in pregnant women.
  
  
  CONCLUSIONS
  There was a greater prevalence of AAV and HPV in pregnant than in non-pregnant women, which suggests that the gestational state may play a role in reactivating the viruses.
authors.affiliation: Center of Infectious Diseases, Health Science Center, Federal University of Espírito Santo, Vitória-ES, Brazil. freitas_lb@yahoo.com.br
authors.forename: Luciana B
authors.initials: LB
authors.lastname: Freitas
authors.forename: Christiane C
authors.initials: CC
authors.lastname: Pereira
authors.forename: Rita
authors.initials: R
authors.lastname: Checon
authors.forename: José Paulo G
authors.initials: JP
authors.lastname: Leite
authors.forename: Jussara P
authors.initials: JP
authors.lastname: Nascimento
authors.forename: Liliana C
authors.initials: LC
authors.lastname: Spano
mesh_terms: D000328:Adult; D001938:Brazil; D002584:Cervix Uteri / Q000821:virology*; D003430:Cross-Sectional Studies; D004279:DNA, Viral / Q000032:analysis; D000229:Dependovirus / Q000145:classification* / Q000235:genetics / Q000472:pathogenicity*; D005260:Female; D006378:Helper Viruses / Q000472:pathogenicity / Q000502:physiology; D006801:Humans; D027383:Papillomaviridae / Q000145:classification* / Q000235:genetics / Q000472:pathogenicity*; D030361:Papillomavirus Infections / Q000175:diagnosis / Q000453:epidemiology; D010322:Parvoviridae Infections / Q000175:diagnosis / Q000453:epidemiology; D011247:Pregnancy; D011251:Pregnancy Complications, Infectious / Q000175:diagnosis / Q000453:epidemiology; D015995:Prevalence; D012189:Retrospective Studies
chemical_list: D004279:DNA, Viral