Histone chaperone as coactivator of chromatin transcription: role of acetylation.
Histone chaperones are a group of histone-interacting proteins, involved in several important cellular functions. These chaperones are essential to facilitate ordered assembly of nucleosomes, both in replication dependent and independent manner. Replication independent function of histone chaperone is necessary for histone eviction during transcriptional initiation and elongation. In this chapter we have discussed a method to evaluate the role of histone chaperone NPM1 (the only known chaperone to get acetylated with functional consequence) in the transcriptional activation which is acetylation dependent.
Gadad
Shrikanth S
SS
Transcription and Disease Laboratory, Molecular Biology and Genetics Unit, Jawaharlal Nehru Centre for Advanced Scientific Research, Jakkur, Bangalore, India.
Shandilya
Jayasha
J
Swaminathan
Venkatesh
V
Kundu
Tapas K
TK
D000107:Acetylation; D000818:Animals; D001681:Biological Assay; D002457:Cell Extracts; D002467:Cell Nucleus / Q000378:metabolism; D002843:Chromatin / Q000235:genetics*; D042002:Chromatin Assembly and Disassembly; D004264:DNA Topoisomerases, Type I / Q000302:isolation & purification; D004331:Drosophila melanogaster; D006367:HeLa Cells; D006657:Histones / Q000302:isolation & purification / Q000378:metabolism*; D006801:Humans; D008565:Membrane Proteins / Q000302:isolation & purification / Q000378:metabolism; D051379:Mice; D008836:Micrococcal Nuclease / Q000378:metabolism; D008967:Molecular Biology / Q000379:methods*; D018832:Molecular Chaperones / Q000378:metabolism*; D009687:Nuclear Proteins / Q000302:isolation & purification / Q000378:metabolism; D000090243:Nucleophosmin; D010957:Plasmids / Q000302:isolation & purification; D011485:Protein Binding; D013698:Templates, Genetic; D014158:Transcription, Genetic
D002457:Cell Extracts; D002843:Chromatin; D006657:Histones; D008565:Membrane Proteins; D018832:Molecular Chaperones; C000716967:NPM1 protein, human; C502089:Nckap1 protein, mouse; C000715570:Npm1 protein, mouse; D009687:Nuclear Proteins; D000090243:Nucleophosmin; D008836:Micrococcal Nuclease; D004264:DNA Topoisomerases, Type I