title: Heterologous hybridization to a complementary DNA  microarray reveals the effect of thermal acclimation in  the endothermic bluefin tuna (Thunnus orientalis).
abstract: The temperature stress that pelagic fishes experience can induce physiological and behavioural changes that leave a signature in gene expression profiles. We used a functional genomics approach to identify genes that were up- or down-regulated following thermal stress in the Pacific bluefin tuna. Following the acclimation period, 113, 81 and 196 genes were found to be differentially expressed between the control (20 degrees C) and cold (15 degrees) treatment groups, in ventricle, red muscle and white muscle, respectively. The genes whose expression levels were responsive to thermal acclimation varied according to muscle fibre type, perhaps reflecting the tissue-specific degrees of endothermy characteristic of this species.
authors.affiliation: Hopkins Marine Station, Stanford University, Pacific Grove, CA 93950, USA. pccastilho@gmail.com
authors.forename: Pedro C
authors.initials: PC
authors.lastname: Castilho
authors.forename: Bradley A
authors.initials: BA
authors.lastname: Buckley
authors.forename: George
authors.initials: G
authors.lastname: Somero
authors.forename: Barbara A
authors.initials: BA
authors.lastname: Block
mesh_terms: D000064:Acclimatization / Q000235:genetics / Q000502:physiology*; D000818:Animals; D001833:Body Temperature Regulation / Q000235:genetics / Q000502:physiology*; D003080:Cold Temperature; D029941:Fish Proteins / Q000235:genetics; D020869:Gene Expression Profiling; D009132:Muscles / Q000502:physiology*; D020411:Oligonucleotide Array Sequence Analysis; D014413:Tuna / Q000235:genetics / Q000502:physiology*
chemical_list: D029941:Fish Proteins