Analysis of the conservation of T cell receptor alpha and beta chain variable regions gene in pp65 peptide-specific HLA-A*0201-restricted CD8+ T cells.
Many viral epitope specific T cell receptors (TCRs) in MHC-matched individuals have been demonstrated to involve conserved amino acid motifs in beta chain complementarity-determining region 3 (CDR3). However, it is not sure whether the conserved motifs can also be found in TCR beta chain. In previous studies, we developed a modified method to enlarge the percentage of cytomegalovirus (CMV) pp65 peptide-specific CD8(+) T cells in PBMC by continuous peptide stimulation in vitro, which provides sufficient number of specific T cells for detection. In this study, we further analyzed the restrictive usage of TCR V alpha and V beta gene families and investigated the CDR3 gene sequence of pp65 peptide-specific CD8(+) T cells. Analysis of CDR3 spectratypes suggested a restricted usage of TCR alpha chain AV8, AV12, AV21, AV31 families and TCR beta chain BV3, BV14, BV21, BV23, BV11 families in donor CD8(+) T cells stimulated by pp65 peptide. The sequences of these T cells involved similar sequence (TX) G (X) A in CDR3 region of TCR alpha chain and L (XT) G (X) A in TCR beta chain.
Luo
Wei
W
Institute of Molecular Immunology, School of Biotechnology, Southern Medical University, Guangzhou 510515, China.
Ma
Li
L
Wen
Qian
Q
Zhou
Mingqian
M
Wang
Xiaoning
X
D000328:Adult; D020816:Amino Acid Motifs; D022801:Complementarity Determining Regions / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D003587:Cytomegalovirus / Q000276:immunology*; D005260:Female; D019673:Genes, T-Cell Receptor alpha / Q000235:genetics; D019674:Genes, T-Cell Receptor beta / Q000235:genetics; D015234:HLA-A Antigens / Q000378:metabolism; D015789:HLA-A2 Antigen; D006801:Humans; D008297:Male; D010455:Peptides / Q000737:chemistry / Q000378:metabolism*; D010750:Phosphoproteins / Q000737:chemistry / Q000276:immunology / Q000378:metabolism*; D011485:Protein Binding; D017422:Sequence Analysis, DNA; D037182:T-Cell Antigen Receptor Specificity; D014763:Viral Matrix Proteins / Q000737:chemistry / Q000276:immunology / Q000378:metabolism*
D022801:Complementarity Determining Regions; D015234:HLA-A Antigens; C435939:HLA-A*02:01 antigen; D015789:HLA-A2 Antigen; D010455:Peptides; D010750:Phosphoproteins; D014763:Viral Matrix Proteins; C065418:cytomegalovirus matrix protein 65kDa