Calcium phosphate/DNA co-precipitates encapsulated fast-degrading polymer films for substrate-mediated gene delivery.
Calcium-phosphate/deoxyribose nucleic acid (Ca-P/DNA) co-precipitates were deposited on or encapsulated in fast-degrading polymer films with surface erosion degradation mechanism to mediate cell transfection. The polymer, cholic acid functionalized star poly(DL-lactide), was synthesized through the ring-opening polymerization of DL-lactide initiated by cholic acid. The releases of DNA from the Ca-P/DNA co-precipitates deposited film and the Ca-P/DNA co-precipitates encapsulated film were determined and compared. The in vitro gene transfections of HEK293 cells, Hela cells, and NIH 3T3 cells showed that the expression of pGL3-Luc plasmid could be effectively mediated by the Ca-P/DNA co-precipitates deposited and encapsulated polymer films. In addition, the films did not exhibit any additional cytotoxicity to the cells during the transfections, indicating that the fast-degrading polymer films have great potential in localized gene delivery.
Zhang
Qiao
Q
Department of Chemistry, Key Laboratory of Biomedical Polymers of Ministry of Education, Wuhan University, Wuhan 430072, People's Republic of China.
Zhao
Dong
D
Zhang
Xian-Zheng
XZ
Cheng
Si-Xue
SX
Zhuo
Ren-Xi
RX
D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry; D002130:Calcium Phosphates / Q000737:chemistry*; D002460:Cell Line; D019826:Cholic Acid / Q000737:chemistry; D004247:DNA / Q000737:chemistry*; D018014:Gene Transfer Techniques; D006801:Humans; D008422:Materials Testing; D051379:Mice; D015394:Molecular Structure; D011091:Polyesters / Q000737:chemistry; D011108:Polymers / Q000737:chemistry*; D013499:Surface Properties; D014162:Transfection / Q000379:methods*
D001672:Biocompatible Materials; D002130:Calcium Phosphates; D011091:Polyesters; D011108:Polymers; C033616:poly(lactide); D004247:DNA; C020243:calcium phosphate; D019826:Cholic Acid