Pretransplant pharmacodynamic analysis of immunosuppressive agents using CFSE-based T-cell proliferation assay.
The impact of pretransplant T-cell sensitivity testing using carboxylfluorescein diacetate succinimidyl ester (CFSE)-based flow cytometry was studied in 32 patients with chronic renal failure. There was considerable interindividual variation in the inhibitory effects of cyclosporine (CSA), tacrolimus (TAC), and prednisolone (PRD) but only a small amount of interindividual variation for mycophenolic acid (MPA). Patients with high sensitivity to CSA tended to experience viral reactivation. In addition to post-transplant blood-level monitoring, pretransplant pharmacodynamics could provide useful information on optimal and safe immunosuppressive therapy.
Kurata
Y
Y
Department of Clinical Pharmacy, College of Pharmacy, Kinjo Gakuin University, Nagoya, Japan.
Kato
M
M
Kuzuya
T
T
Miwa
Y
Y
Iwasaki
K
K
Haneda
M
M
Amioka
K
K
Watarai
Y
Y
Uchida
K
K
Nakao
A
A
Kobayashi
T
T
D000328:Adult; D049109:Cell Proliferation / Q000187:drug effects; D016572:Cyclosporine / Q000493:pharmacokinetics / Q000494:pharmacology; D005260:Female; D005452:Fluoresceins; D005456:Fluorescent Dyes; D006801:Humans; D007166:Immunosuppressive Agents / Q000493:pharmacokinetics* / Q000494:pharmacology*; D007676:Kidney Failure, Chronic / Q000601:surgery; D016030:Kidney Transplantation / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D011239:Prednisolone / Q000493:pharmacokinetics / Q000494:pharmacology; D011446:Prospective Studies; D013388:Succinimides; D013601:T-Lymphocytes / Q000187:drug effects* / Q000276:immunology; D016559:Tacrolimus / Q000493:pharmacokinetics / Q000494:pharmacology
C087165:5-(6)-carboxyfluorescein diacetate succinimidyl ester; D005452:Fluoresceins; D005456:Fluorescent Dyes; D007166:Immunosuppressive Agents; D013388:Succinimides; D016572:Cyclosporine; D011239:Prednisolone; D016559:Tacrolimus