title: Identification of Streptococcus agalactiae isolates from various phylogenetic lineages by matrix-assisted laser desorption ionization-time of flight mass spectrometry.
abstract: Variations in proteins related to bacterial diversity may affect species identification performed using matrix-assisted laser desorption ionization (MALDI)-time of flight mass spectrometry. Using this method, we identified 110 Streptococcus agalactiae isolates characterized by serotyping and multilocus sequence typing. Serotype III and sequence type 23 strains expressed the widest variation in molecular weight of putative "species-identifying" biomarker ions. Recognition of the diversity of MALDI patterns observed in strains that represent all major intraspecies lineages assists in the constitution of an optimal reference database.
authors.affiliation: Université François-Rabelais de Tours, UFR de Médecine, EA 3854 Bactéries et risque materno-foetal, Tours, France. lartigue@med.univ-tours.fr
authors.forename: Marie-Frédérique
authors.initials: MF
authors.lastname: Lartigue
authors.forename: Geneviève
authors.initials: G
authors.lastname: Héry-Arnaud
authors.forename: Eve
authors.initials: E
authors.lastname: Haguenoer
authors.forename: Anne-Sophie
authors.initials: AS
authors.lastname: Domelier
authors.forename: Pierre-Olivier
authors.initials: PO
authors.lastname: Schmit
authors.forename: Nathalie
authors.initials: N
authors.lastname: van der Mee-Marquet
authors.forename: Philippe
authors.initials: P
authors.lastname: Lanotte
authors.forename: Laurent
authors.initials: L
authors.lastname: Mereghetti
authors.forename: Markus
authors.initials: M
authors.lastname: Kostrzewa
authors.forename: Roland
authors.initials: R
authors.lastname: Quentin
mesh_terms: D001426:Bacterial Proteins / Q000032:analysis*; D016000:Cluster Analysis; D016172:DNA Fingerprinting; D005260:Female; D014644:Genetic Variation; D005838:Genotype; D006801:Humans; D012703:Serotyping; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D013292:Streptococcus agalactiae / Q000737:chemistry* / Q000145:classification / Q000302:isolation & purification*
chemical_list: D001426:Bacterial Proteins