[Effect of exopolysaccharide from Bifidobacterium bifidum on cell of gastric cancer and human telomerase reverse transcriptase].
OBJECTIVE
To study the inhibition of human gastric cancer cell line BGC-823 by exopolysaccharide (B. EPS) extracted from Bifidobacterium bifidum and the effect on the activity of telomerase rate-limiting factor human telomerase reverse transcriptase (hTERT).


METHODS
In vitro human gastric cancer cell line BGC-823 were treated by three concentrations of B. EPS. The inhibition rate of gastric cancer cell line BGC-823, the morphology changes of cells and the apoptosis in the initial stage of cell was observed respectively by MTF, inverted fluorescent microscope and the Annexin V-FITC/PI flow cytometry. In addition, the effect of B. EPS on cell telomerase rate-limiting factor hTERT mRNA was tested by RT-PCR; the changes in the concentration of calcium ions inside the cytoplasm were detected by spectrofluorometer.


RESULTS
B. EPS inhibited the growth of gastric cancer cell BGC-823 (P < 0.05), the inhibition rate increased and the concentration of B.EPS showed dose-time response relation. The expression of cell telomerase rate-limiting factor hTERT mRNA decrease after effected by B.EPS (P < 0.05), and showed dose-effect relation with B. EPS. The concentration of calcium ion in cytoplasm was higher than the control apparently (P < 0.05).


CONCLUSIONS
The apoptosis mechanism of human gastric cancer cell line BGC-823 induced by B. EPS might be relevant with the changes of the hTERT mRNA expression and the calcium ions concentration in cytoplasm.
Chen
Xu
X
Key Laboratory of Dairy Science, Ministry of Education, China. chenxu19800203@126.com
Jiang
Hongrui
H
Yang
Yanmei
Y
Liu
Ning
N
D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D001644:Bifidobacterium / Q000737:chemistry*; D049109:Cell Proliferation / Q000187:drug effects; D005753:Gastric Mucosa / Q000187:drug effects*; D006801:Humans; D011135:Polysaccharides, Bacterial / Q000494:pharmacology*; D012333:RNA, Messenger / Q000378:metabolism; D013274:Stomach Neoplasms / Q000473:pathology*; D019098:Telomerase / Q000187:drug effects* / Q000378:metabolism; D016615:Telomere / Q000201:enzymology
D000970:Antineoplastic Agents; D011135:Polysaccharides, Bacterial; D012333:RNA, Messenger; C406479:exopolysaccharide B39; C509186:TERT protein, human; D019098:Telomerase