title: Analysis of calvarial bone defects in rats using microcomputed tomography: potential for a novel composite material and a new quantitative measurement.
abstract: Reconstruction of craniomaxillofacial defects is a challenge for surgeons and has psychological and functional burdens for patients. Undoubtedly, there is a need for improved biomaterials and techniques for craniomaxillofacial reconstruction. We assessed the potential regeneration of bone using three modifications of a novel composite and explored the validity of a new measurement using microcomputed tomography (micro-CT). We placed three different composite samples in calvarial defects in rats and analysed healing with micro-CT. The results showed that polycaprolactone (PCL) with phosphate glass fibre is promising for non-load bearing applications in the craniomaxillofacial region. Also, the new micro-CT measurement of the temporal characterisation of the mineralisation of bone (TCBM) has the potential to evolve into a reliable predictor of bony healing and its quality.
authors.affiliation: Oral and Maxillofacial Surgery, Leeds Dental Institute, University of Leeds, Leeds, UK. cefeoglu@yahoo.com
authors.forename: Candan
authors.initials: C
authors.lastname: Efeoglu
authors.forename: Julie L
authors.initials: JL
authors.lastname: Burke
authors.forename: Andrew J
authors.initials: AJ
authors.lastname: Parsons
authors.forename: Graham A
authors.initials: GA
authors.lastname: Aitchison
authors.forename: Colin
authors.initials: C
authors.lastname: Scotchford
authors.forename: Chris
authors.initials: C
authors.lastname: Rudd
authors.forename: Aditya
authors.initials: A
authors.lastname: Vikram
authors.forename: Sheila E
authors.initials: SE
authors.lastname: Fisher
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry / Q000627:therapeutic use*; D015519:Bone Density / Q000502:physiology; D001847:Bone Diseases / Q000000981:diagnostic imaging / Q000601:surgery*; D001861:Bone Regeneration / Q000502:physiology; D018786:Bone Substitutes / Q000737:chemistry / Q000627:therapeutic use*; D016025:Bone Transplantation / Q000000981:diagnostic imaging / Q000473:pathology; D002113:Calcification, Physiologic / Q000502:physiology; D002516:Ceramics / Q000737:chemistry / Q000627:therapeutic use; D005898:Glass / Q000737:chemistry; D021621:Imaging, Three-Dimensional / Q000379:methods; D010710:Phosphates / Q000737:chemistry; D011091:Polyesters / Q000737:chemistry / Q000627:therapeutic use; D051381:Rats; D017208:Rats, Wistar; D019651:Plastic Surgery Procedures / Q000379:methods; D012886:Skull / Q000000981:diagnostic imaging / Q000601:surgery*; D013997:Time Factors; D014945:Wound Healing / Q000502:physiology; D055114:X-Ray Microtomography / Q000379:methods
chemical_list: D001672:Biocompatible Materials; D018786:Bone Substitutes; D010710:Phosphates; D011091:Polyesters; C425330:bioactive glass 45S5; C009753:fiberglass; C016240:polycaprolactone