title: Analysis of DNA topology in yeast chromatin.
abstract: Topology of closed circular DNA is affected by its packaging into nucleosomes and potentially by alteration of nucleosome structure. Changes in topology that reflect alterations in chromatin structure can be measured and quantified using closed circular plasmids from living yeast. Here we describe detailed protocols for measuring DNA topology in yeast chromatin.
authors.affiliation: NY State Department of Health and SUNY School of Public Health, Wadsworth Center, Albany, NY, USA.
authors.forename: Randall H
authors.initials: RH
authors.lastname: Morse
mesh_terms: D002843:Chromatin / Q000737:chemistry*; D004271:DNA, Fungal / Q000737:chemistry*; D008967:Molecular Biology / Q000379:methods*; D012441:Saccharomyces cerevisiae / Q000737:chemistry*; D013045:Species Specificity
chemical_list: D002843:Chromatin; D004271:DNA, Fungal