[A simple and efficient method for establishing a mouse model of orthotopic MB49 bladder cancer].
OBJECTIVE
To establish a simple and efficient method for establishing a mouse model of orthotopic superficial bladder cancer.


METHODS
C57BL/6 mice were anesthetized with sodium pentobarbital and catheterized with modified IV catheter (24 G). The mice were intravesically pretreated with HCl and then with NaOH, and after washing the bladders with phosphate-buffered saline (PBS), 100 microl (1 x 10(7)) MB49 cells were infused and allowed to incubate in the bladder for 2 h followed intravesical mitomycin C (MMC) administration. The tumor formation rate, survival, gross hematuria, and bladder weight were determined as the outcome variables, and the pathology of the bladders was observed.


RESULTS
Instillation of MB49 tumor cells resulted in a tumor formation rates of 100% in all the pretreated groups while 0% in the control group without pretreatment. MMC significantly reduced the bladder weight as compared to PBS.


CONCLUSIONS
We have successfully established a stable, reproducible, and reliable orthotopic bladder cancer model in mice.
Liang
Zhong-kun
ZK
Department of Urologic Surgery, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China. liangzhongkun@139.com
Zhang
Lin
L
Hu
Zhi-ming
ZM
Chen
Zhong
Z
Huang
Xin
X
Shi
Xiang-hua
XH
Tan
Wan-long
WL
Gao
Ji-min
JM
D000818:Animals; D045744:Cell Line, Tumor; D002471:Cell Transformation, Neoplastic; D004195:Disease Models, Animal; D005260:Female; D051379:Mice; D008810:Mice, Inbred C57BL; D016685:Mitomycin / Q000494:pharmacology; D009929:Organ Size / Q000187:drug effects; D001743:Urinary Bladder / Q000187:drug effects / Q000473:pathology; D001749:Urinary Bladder Neoplasms / Q000473:pathology
D016685:Mitomycin