Identification of promoters for efficient gene expression in Magnetospirillum gryphiswaldense.
To develop an expression system for the magnetotactic bacterium Magnetospirillum gryphiswaldense, we compared gene expression from the widely used Escherichia coli P(lac) promoter with that from known and predicted genuine M. gryphiswaldense promoters. With the use of green fluorescent protein as a reporter, the highest expression level was observed with the magnetosomal P(mamDC) promoter. We demonstrate that this promoter can be used for the expression of modified magnetosome proteins to generate "antibody-binding" magnetosomes.
Lang
Claus
C
Ludwig-Maximillians-Universität München, Department Biologie I, Bereich Mikrobiologie, Planegg-Martinsried, Germany.
Pollithy
Anna
A
Schüler
Dirk
D
D004926:Escherichia coli / Q000235:genetics; D015870:Gene Expression; D017930:Genes, Reporter; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D042201:Magnetospirillum / Q000235:genetics*; D008967:Molecular Biology / Q000379:methods*; D011401:Promoter Regions, Genetic
D049452:Green Fluorescent Proteins