title: Vaccination strategies to enhance local immunity and protection against Mycobacteriun tuberculosis.
abstract: To determine the immunogenicity and protective efficacy of the Mycobacterium tuberculosis 10 kD culture filtrate protein (CFP10), and to evaluate strategies that enhance local immunity, we used C57Bl/6 DR4 mice that were transgenic for human HLA DRB1 0401, because CFP10 contains epitopes for DRB1 0401 but not for C57Bl/6 mice. Intramuscular immunization with a DNA vaccine encoding CFP10 elicited production of IFN-gamma by systemic CD4+ T cells, and one intravenous dose of the CFP10-based DNA vaccine coated with polyethylenimine (PEI) stimulated IFN-gamma production by lung CD4+ cells and reduced the pulmonary bacillary burden. We conclude that CFP10 is a potential vaccine candidate and that coating vaccines with PEI enhances local protective immunity to tuberculosis
authors.affiliation: Center for Pulmonary and Infectious Disease Control, University of Texas Health Science Center at Tyler, Tyler, TX 75708, United States.
authors.forename: Peter
authors.initials: P
authors.lastname: Klucar
authors.forename: Peter F
authors.initials: PF
authors.lastname: Barnes
authors.forename: Ying
authors.initials: Y
authors.lastname: Kong
authors.forename: Susan T
authors.initials: ST
authors.lastname: Howard
authors.forename: Xiuhua
authors.initials: X
authors.lastname: Pang
authors.forename: Fang-Fang
authors.initials: FF
authors.lastname: Huang
authors.forename: Amy R
authors.initials: AR
authors.lastname: Tvinnereim
authors.forename: Buka
authors.initials: B
authors.lastname: Samten
authors.forename: Homayoun
authors.initials: H
authors.lastname: Shams
mesh_terms: D000818:Animals; D000942:Antigens, Bacterial / Q000235:genetics / Q000276:immunology; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D015798:HLA-DR1 Antigen / Q000276:immunology; D007114:Immunization; D007371:Interferon-gamma / Q000276:immunology; D008168:Lung / Q000166:cytology / Q000276:immunology; D052119:Lysosomal-Associated Membrane Protein 2 / Q000276:immunology; D051907:Lysosomal Membrane Proteins / Q000276:immunology; D008247:Lysosomes / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D009169:Mycobacterium tuberculosis / Q000276:immunology*; D010446:Peptide Fragments / Q000276:immunology; D010957:Plasmids / Q000276:immunology; D011094:Polyethyleneimine / Q000737:chemistry; D013154:Spleen / Q000166:cytology / Q000276:immunology; D014397:Tuberculosis, Pulmonary / Q000276:immunology* / Q000517:prevention & control*; D019444:Vaccines, DNA / Q000276:immunology; D014614:Vaccines, Synthetic / Q000276:immunology; D052118:Lysosomal-Associated Membrane Protein 1
chemical_list: D000942:Antigens, Bacterial; D015798:HLA-DR1 Antigen; D007371:Interferon-gamma; D052119:Lysosomal-Associated Membrane Protein 2; D051907:Lysosomal Membrane Proteins; D010446:Peptide Fragments; D011094:Polyethyleneimine; D019444:Vaccines, DNA; D014614:Vaccines, Synthetic; C489634:CFP-10 protein (71-85), Mycobacterium tuberculosis; C052255:Lamp1 protein, mouse; D052118:Lysosomal-Associated Membrane Protein 1