Expression of IGF-1R and iNOS in nasal polyps; epithelial cell homeostasis and innate immune mechanisms in pathogenesis of nasal polyposis.
Nasal polyps (NP), edematous projections of nasal mucosa (NM), are characterized by an inflammatory cellular infiltrate, however, little is known about etiopathogenesis of NP. Both innate immune mechanisms leading to activation of NF-kappaB and homeostasis of epithelial cells were implicated in the pathogenesis of NP. In this study we investigated the expression of insulin-like growth factor-1 receptor (IGF-1R) and inducible nitric-oxide synthase (iNOS) in NP compared to healthy NM in both the epithelial and stromal compartments. Using immunohistochemistry, frozen tissue sections of NP from 18 patients, and mucosal biopsy specimens of the inferior turbinate from 17 subjects were stained for IGF-1R and iNOS markers. Fluorescence microscopy and computerized image analysis revealed low numbers of IGF-1R-positive cells in all specimens. However, substantially increased numbers of IGF-1R-positive cells were found in NP compared to NM both within the epithelium (1.63 vs. 0.43) and stroma (3.27 vs. 1.03). Positivity for iNOS was detected within the epithelium of NP compared with NM. Numbers of iNOS-positive single cells were highly increased in NP vs. NM in both epithelial (3.83 vs. 1.08) and stromal (4.96 vs. 2.67) compartments. An increased iNOS expression within the epithelial layer as well as increased number of iNOS- and IGF-1R-positive cells in NP was observed. This suggests that innate immune mechanism, and to a lesser extent also growth and homeostasis of epithelial cells, may play a role in formation of NP.
Fundová
P
P
Ear, Nose and Throat Department, Central Military Hospital, Prague, Czech Republic. petra.fundova@uvn.cz
Filipovský
T
T
Funda
D P
DP
Hovorka
O
O
Holý
R
R
Navara
M
M
Tlaskalová-Hogenová
H
H
D001706:Biopsy; D016207:Cytokines / Q000378:metabolism; D004781:Environmental Exposure; D004847:Epithelial Cells / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D006706:Homeostasis; D006801:Humans; D007113:Immunity, Innate; D008856:Microscopy, Fluorescence; D016328:NF-kappa B / Q000378:metabolism; D009297:Nasal Mucosa / Q000737:chemistry; D009298:Nasal Polyps / Q000737:chemistry* / Q000209:etiology / Q000276:immunology; D052247:Nitric Oxide Synthase Type II / Q000032:analysis*; D017526:Receptor, IGF Type 1 / Q000032:analysis*; D016037:Single-Blind Method; D017154:Stromal Cells / Q000276:immunology / Q000378:metabolism / Q000473:pathology
D016207:Cytokines; D016328:NF-kappa B; C496319:NOS2 protein, human; D052247:Nitric Oxide Synthase Type II; D017526:Receptor, IGF Type 1