Determination of bencycloquidium bromide in human urine using weak cation-exchange solid-phase extraction and LC-ESI-MS: Method validation and application to kinetic study of urinary excretion.
A sensitive LC-ESI-MS method has been developed and validated for the determination of bencycloquidium bromide (BCQB) in human urine samples. The method utilized a solid-phase extraction (SPE) procedure, choosing carboxy propyl phase (CBA) as the extracting sorbent for purification of BCQB, with better baseline and higher selectivity achieved. Sample preparation by this method yielded very good and consistent mean recovery of above 94.5%. Another major benefit of the present method was the high detectability, with a lower limit of quantification (LLOQ) of 0.02ng/ml. The developed method was successfully applied to determine BCQB in human urine, and was proved to be suitable for use in Phase I clinical pharmacokinetic study of BCQB.
Zhou
Wenjia
W
Department of Pharmaceutical Analysis, China Pharmaceutical University, Nanjing, China.
Ding
Li
L
Xu
Guili
G
Wang
Yongqing
Y
Sun
Luning
L
He
Jianchang
J
Huang
Yao
Y
Hu
Linlin
L
Chen
Xiaoping
X
D019086:Bridged Bicyclo Compounds, Heterocyclic / Q000652:urine*; D002411:Cation Exchange Resins; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D007700:Kinetics; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
D019086:Bridged Bicyclo Compounds, Heterocyclic; D002411:Cation Exchange Resins; C518439:bencycloquidium bromide