[Effect of rat recombinant leptin on expression of SOCS-3 in mature adipocytes].
OBJECTIVE
To study the effect of rat recombinant leptin on expressions of suppressor of cytokine signaling 3 (SOCS-3) mRNA and protein in mature adipocytes .


METHODS
Mature adipocytes were separated from epididymal adipose tissues of male SD rats, and the expressions of long-form leptin receptor mRNA were detected. The separated adipocytes were then treated with rat recombinant leptin at the doses of 0.5-500 nmol/L for 0.5 to 6h. The expressions of SOCS-3 mRNA were measured with RT-PCR and proteins were measured with immuniprecipitation combined with Western blot analysis.


RESULTS
Low-level expressions of long-form leptin receptor mRNA could be measured in mature adipocytes. The adipocytes expressed small amounts of SOCS-3 mRNA and protein witbout leptin treatment, and no significant changes were observed at 0.5 Snmol/L of leptin. However, tbe expressions of SOCS-3 mRNA and protein increased significantly at the doses of 5-500 nmol/L of leptin in the consistent with treatment time.


CONCLUSIONS
Leptin could have direct effects on adipocytes. High leptin concentration could induce the expressions of SOCS-3 mRNA and protein in mature adipocytes.
Liu
Li
L
School of Public Health, China Medical University, Shenyang 110001, China. liuli@mail.cmu.edu.cn
Gu
Hailun
H
Yang
Jun
J
Yu
Fei
F
D017667:Adipocytes / Q000166:cytology / Q000378:metabolism*; D000818:Animals; D020738:Leptin / Q000494:pharmacology*; D008297:Male; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D054411:Receptors, Leptin / Q000235:genetics / Q000378:metabolism; D011994:Recombinant Proteins / Q000494:pharmacology; D000071223:Suppressor of Cytokine Signaling 3 Protein; D050826:Suppressor of Cytokine Signaling Proteins / Q000235:genetics / Q000378:metabolism*
D020738:Leptin; D012333:RNA, Messenger; D054411:Receptors, Leptin; D011994:Recombinant Proteins; C491410:Socs3 protein, rat; D000071223:Suppressor of Cytokine Signaling 3 Protein; D050826:Suppressor of Cytokine Signaling Proteins