title: Isolation of neural stem/progenitor cells by using EGF/FGF1 and FGF1B promoter-driven green fluorescence from embryonic and adult mouse brains.
abstract: Fibroblast growth factor 1 (FGF1) and FGF2 have been shown to maintain the proliferation, self-renewal and multipotent capacities of neural stem/progenitor cells (NSPCs) in vitro. FGF1 is unique for binding to all known FGF receptors. In this study, we investigated if exogenous EGF and FGF1 could be used in the isolation of NSPCs from embryonic mouse brains. We demonstrated that EGF/FGF1-responsive cells exhibited lower proliferation rate and neurosphere formation efficiency than EGF/FGF2-responsive NSPCs. However, EGF/FGF1-responsive mouse brain cells exhibited better neural differentiation capacities than EGF/FGF2-responsive NSPCs at E11.5. Using F1BGFP reporter, we further demonstrated that F1BGFP+ cells showed similar multipotent capacities to CD133+ NSPCs, and could be induced more efficiently toward neuronal differentiation. Our results suggested that EGF/FGF1-responsive cells from E11.5 mouse brains could self-renew and have better multipotency than EGF/FGF2-responsive NSPCs. Further, CD133+ and F1BGFP+ NSPCs may also represent different subsets of NSPCs during neural development and adult neurogenesis.
authors.affiliation: Institute of Cellular and System Medicine, National Health Research Institutes, Miaoli 35053, Taiwan, ROC.
authors.forename: Don-Ching
authors.initials: DC
authors.lastname: Lee
authors.forename: Yi-Chao
authors.initials: YC
authors.lastname: Hsu
authors.forename: Yu-Fen
authors.initials: YF
authors.lastname: Chung
authors.forename: Chao-Yang
authors.initials: CY
authors.lastname: Hsiao
authors.forename: Su-Liang
authors.initials: SL
authors.lastname: Chen
authors.forename: Mei-Shu
authors.initials: MS
authors.lastname: Chen
authors.forename: Hua-Kuo
authors.initials: HK
authors.lastname: Lin
authors.forename: Ing-Ming
authors.initials: IM
authors.lastname: Chiu
mesh_terms: D053687:Adult Stem Cells / Q000166:cytology / Q000378:metabolism*; D000818:Animals; D001921:Brain / Q000166:cytology* / Q000196:embryology / Q000378:metabolism; D002454:Cell Differentiation; D002469:Cell Separation / Q000379:methods; D053595:Embryonic Stem Cells / Q000166:cytology / Q000378:metabolism*; D004815:Epidermal Growth Factor / Q000378:metabolism*; D016220:Fibroblast Growth Factor 1 / Q000378:metabolism*; D016222:Fibroblast Growth Factor 2 / Q000378:metabolism; D049452:Green Fluorescent Proteins; D006801:Humans; D051379:Mice; D009474:Neurons / Q000166:cytology / Q000378:metabolism; D011401:Promoter Regions, Genetic; D051496:Receptor, Fibroblast Growth Factor, Type 1 / Q000235:genetics / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D012038:Regeneration
chemical_list: D011993:Recombinant Fusion Proteins; D016222:Fibroblast Growth Factor 2; D016220:Fibroblast Growth Factor 1; D049452:Green Fluorescent Proteins; D004815:Epidermal Growth Factor; D051496:Receptor, Fibroblast Growth Factor, Type 1