title: [The frequency of small colony variants of Staphylococcus aureus strains isolated from children with cystic fibrosis].
abstract: The aim of this study was to determine the frequency of spontaneous and inducible SCV formation in S. aureus isolates obtained from CF patients. A total of 297 S. aureus strains isolated from 33 CF patients, and 40 isolates obtained from healthy control children, were analysed. S. aureus was cultured on Columbia blood agar, Schaedler agar and Chapman agar under aerophilic and microaerophilic conditions. Subinhibitory gentamicin concentrations (1 mg/L) were used to test S. aureus ability to form SCVs. The study showed that the characteristic feature of S. aureus strains, persistently colonizing the airways of CS patients, was the formation of small colony variants. In the subinhibitory gentamicin concentration, S. aureus strains from CS patients formed SCVs more frequently (55%) than isolates from healthy subjects (20%). SCV formation in CF patients was associated with treatment with inhaled aminoglycosides.
authors.affiliation: Zakład Mikrobiologii i Immunologii Klinicznej, Instytut Pomnik Centrum Zdrowia Dziecka Warszawal.
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Semczuk
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Dzierianowska-Fangrat
authors.forename: Hanna
authors.initials: H
authors.lastname: Dmeńska
authors.forename: Danuta
authors.initials: D
authors.lastname: Dzierzanowska
mesh_terms: D000293:Adolescent; D002648:Child; D015169:Colony Count, Microbial; D003550:Cystic Fibrosis / Q000382:microbiology*; D006801:Humans; D012137:Respiratory System / Q000382:microbiology*; D013045:Species Specificity; D013211:Staphylococcus aureus / Q000145:classification* / Q000302:isolation & purification