Real-time in vivo imaging of adult zebrafish brain using optical coherence tomography.
We report noninvasive imaging of the brain of adult zebrafish (Danio rerio) using real time optical coherence tomography (OCT) capable of acquiring cross sectional 2D OCT images @ 8 frames/sec. Anatomic features such as telencephalon, tectum opticum, eminentia Granularis and cerebellum were clearly resolved in the OCT images. A 3D model of zebrafish brain was reconstructed, for the first time to our knowledge, using these 2D OCT images.
Rao
K Divakar
KD
Laser Biomedical Applications & Instrumentation Division, Raja Ramanna Centre for Advanced Technology, Indore-452013, India. kdivakar@rrcat.gov.in
Alex
Aneesh
A
Verma
Yogesh
Y
Thampi
Sreeja
S
Gupta
Pradeep K
PK
D000818:Animals; D001921:Brain / Q000033:anatomy & histology* / Q000166:cytology; D021621:Imaging, Three-Dimensional; D013997:Time Factors; D041623:Tomography, Optical Coherence / Q000379:methods*; D015027:Zebrafish / Q000033:anatomy & histology*