Origin of nuclear and chromosomal alterations derived from the action of an aneugenic agent--Trifluralin herbicide.
Trifluralin is a herbicide capable of interfering in mitotic cell division due to either microtubule depolymerization or alteration in the concentration of calcium ions within the cell. The aim of this study was to investigate the effects of trifluralin in Allium cepa meristematic cells, evaluating the induction mechanisms of the chromosomal and nuclear aberrations. In this study, A. cepa root tips were submitted for 24h treatment to several concentrations of this herbicide and 48 h recovery post-treatment. The results showed that some concentrations of trifluralin can lead to a mitotic index inhibition, besides inducing chromosomal and nuclear alterations throughout the mitotic cycle. Some of the alterations found seem to be resulting from the herbicide action in different phases and in more than one consecutive cell cycle.
São Paulo State University, Av. 24 A, 1515, Bela Vista, CEP 13506-900 Rio Claro, SP, Brazil.
Thaís Cristina Casimiro
TC
Fernandes
Dânia Elisa Christofoletti
DE
Mazzeo
Maria Aparecida
MA
Marin-Morales
D024422:Aneugens / Q000633:toxicity*; D002869:Chromosome Aberrations; D004249:DNA Damage; D006540:Herbicides / Q000633:toxicity*; D008938:Mitosis / Q000187:drug effects*; D009152:Mutagenicity Tests; D019697:Onions / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D018517:Plant Roots / Q000187:drug effects / Q000378:metabolism; D014274:Trifluralin / Q000633:toxicity*
D024422:Aneugens; D006540:Herbicides; D014274:Trifluralin