Real-time in vivo imaging of adult zebrafish brain using optical coherence tomography.
We report noninvasive imaging of the brain of adult zebrafish (Danio rerio) using real time optical coherence tomography (OCT) capable of acquiring cross sectional 2D OCT images @ 8 frames/sec. Anatomic features such as telencephalon, tectum opticum, eminentia Granularis and cerebellum were clearly resolved in the OCT images. A 3D model of zebrafish brain was reconstructed, for the first time to our knowledge, using these 2D OCT images.
Laser Biomedical Applications & Instrumentation Division, Raja Ramanna Centre for Advanced Technology, Indore-452013, India. kdivakar@rrcat.gov.in
K Divakar
KD
Rao
Aneesh
A
Alex
Yogesh
Y
Verma
Sreeja
S
Thampi
Pradeep K
PK
Gupta
D000818:Animals; D001921:Brain / Q000033:anatomy & histology* / Q000166:cytology; D021621:Imaging, Three-Dimensional; D013997:Time Factors; D041623:Tomography, Optical Coherence / Q000379:methods*; D015027:Zebrafish / Q000033:anatomy & histology*