In vivo magnetic resonance tracking of Feridex-labeled bone marrow-derived neural stem cells after autologous transplantation in rhesus monkey.
Bone marrow stroma cells-derived neural stem cells (BMSCs-D-NSCs) transplantation is a promising strategy for the treatment of nervous system disorders. The development of a non-invasive method to follow the fate of BMSCs-D-NSCs in vivo is very important for the future application of this treatment. In this paper, we show for the first time, that BMSCs-D-NSCs from rhesus monkeys can be labeled in vitro with the superparamagnetic iron oxide (SPIO) contrast agent Feridex and Poly-L-lysine (PLL) without affecting morphology, cell cycle, telomerase activity, proliferation and differentiation ability of the labeled cells. Furthermore, when autografted into the striatum, these cells survived, differentiated and were incorporated into the brain, and could be reliably tracked using MRI, as confirmed by histological examination of the grafting sites with PKH(67) fluorescence. These results suggest that Feridex labeling of BMSCs-D-NSCs is feasible, efficient and safe for MRI tracing following autografting into the rhesus monkey nervous system.
Ke
Yi-quan
YQ
Department of Neurosurgery, Zhujiang Hospital, Southern Medical University, Guangzhou, PR China. yiquanke@yahoo.com.cn
Hu
Chang-chen
CC
Jiang
Xiao-dan
XD
Yang
Zhi-jun
ZJ
Zhang
Han-wei
HW
Ji
Hong-ming
HM
Zhou
Li-yuan
LY
Cai
Ying-qian
YQ
Qin
Ling-sha
LS
Xu
Ru-xiang
RX
D000818:Animals; D017209:Apoptosis; D001854:Bone Marrow Cells / Q000166:cytology* / Q000648:ultrastructure; D001921:Brain / Q000166:cytology* / Q000648:ultrastructure; D002453:Cell Cycle; D002470:Cell Survival; D003911:Dextrans; D052203:Ferrosoferric Oxide; D007150:Immunohistochemistry; D007501:Iron; D008253:Macaca mulatta; D008279:Magnetic Resonance Imaging / Q000379:methods*; D058185:Magnetite Nanoparticles; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D009474:Neurons / Q000166:cytology / Q000502:physiology* / Q000648:ultrastructure; D010087:Oxides; D033581:Stem Cell Transplantation; D013234:Stem Cells / Q000166:cytology / Q000502:physiology* / Q000648:ultrastructure; D017154:Stromal Cells / Q000502:physiology / Q000648:ultrastructure; D019098:Telomerase / Q000378:metabolism; D014182:Transplantation, Autologous
D003911:Dextrans; D058185:Magnetite Nanoparticles; D010087:Oxides; D007501:Iron; D019098:Telomerase; C065507:ferumoxides; D052203:Ferrosoferric Oxide