Confocal light scattering spectroscopic imaging system for in situ tissue characterization.
We report on the design and construction of a confocal light scattering spectroscopic imaging system aimed ultimately to conduct depth-resolved characterization of biological tissues. The confocal sectioning ability of the system is demonstrated using a two-layer sample consisting of a 200 microm thick cancer cell layer on top of a scattering layer doped with a green absorber. The measurement results demonstrate that distinct light scattering signals can be isolated from each layer with an axial and a lateral resolution of 30 and 27 microm, respectively. Such a system is expected to have significant applications in the areas of tissue engineering and disease diagnostics and monitoring.
Department of Biomedical Engineering, Tufts University, 4 Colby Street, Medford, Massachusetts 02155, USA.
Peter
P
Huang
Martin
M
Hunter
Irene
I
Georgakoudi
D045744:Cell Line, Tumor; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006801:Humans; D007089:Image Enhancement / Q000295:instrumentation*; D007938:Leukemia / Q000473:pathology* / Q000503:physiopathology*; D018613:Microscopy, Confocal / Q000295:instrumentation* / Q000379:methods; D009391:Nephelometry and Turbidimetry / Q000295:instrumentation* / Q000379:methods; D013057:Spectrum Analysis / Q000295:instrumentation* / Q000379:methods; D041623:Tomography, Optical Coherence / Q000295:instrumentation* / Q000379:methods