In vivo diagnosis of acute intestinal graft-versus-host disease by confocal endomicroscopy.
OBJECTIVE
Conventional histology with hematoxylin and eosin (H&E) staining is the accepted standard for diagnosing acute intestinal graft-versus-host disease (GvHD). Confocal endomicroscopy (CEM) is a noninvasive method that allows in vivo histology to be performed during endoscopy. The aim of this study was to evaluate CEM for the diagnosis of acute intestinal GvHD.


METHODS
This observational pilot study conducted between September 2006 and August 2008 included patients with acute diarrhea after stem cell transplantation, infectious diarrhea, or active ulcerative colitis. CEM (EC-3870CIFK, Pentax, Tokyo, Japan) was performed after intravenous injection of fluorescein 10% and topical application of acriflavine 0.05%.


RESULTS
A total of 35 patients with acute diarrhea after stem cell transplantation were examined. In 16 patients, CEM and histology showed no evidence of GvHD. In 14/19 patients with histologically confirmed GvHD, the diagnosis could already be established by CEM during ongoing endoscopy. In GvHD grade IV, near complete destruction of the colonic crypts ("flat mucosa") was visible. Control patients with infectious colitis (N = 15) or ulcerative colitis (N = 15) displayed inflammatory changes but no evidence of GvHD. Altogether, sensitivity of CEM was 74% and specificity was 100 %.


CONCLUSIONS
CEM improves rapid diagnosis of acute intestinal GvHD with high accuracy while performing endoscopy. Platelet transfusions and unnecessary biopsy acquisition can be avoided once acute intestinal GvHD has been diagnosed in vivo.
Medical Department I Gastroenterology, Infectious Diseases, Rheumatology, Charité Campus Benjamin Franklin, Berlin, Germany. christian.bojarski@charite.de
C
C
Bojarski
U
U
Günther
K
K
Rieger
F
F
Heller
C
C
Loddenkemper
M
M
Grünbaum
L
L
Uharek
M
M
Zeitz
J C
JC
Hoffmann
D000208:Acute Disease; D000293:Adolescent; D000328:Adult; D017209:Apoptosis / Q000502:physiology; D001706:Biopsy; D002169:Campylobacter Infections / Q000175:diagnosis / Q000473:pathology; D003092:Colitis / Q000175:diagnosis / Q000473:pathology; D003093:Colitis, Ulcerative / Q000175:diagnosis / Q000473:pathology; D020685:Colonoscopes; D003937:Diagnosis, Differential; D020345:Enterocolitis, Necrotizing / Q000175:diagnosis / Q000473:pathology; D005260:Female; D006086:Graft vs Host Disease / Q000175:diagnosis* / Q000473:pathology*; D006801:Humans; D007413:Intestinal Mucosa / Q000473:pathology*; D008297:Male; D018613:Microscopy, Confocal / Q000295:instrumentation*; D008875:Middle Aged; D012680:Sensitivity and Specificity; D033581:Stem Cell Transplantation; D055815:Young Adult