Cadmium and mineral nutrient accumulation in potato plantlets grown under cadmium stress in two different experimental culture conditions.
In order to evaluate the effect of cadmium (Cd(2+)) toxicity on mineral nutrient accumulation in potato (Solanum tuberosum L.), two cultivars named Asterix and Macaca were cultivated both in vitro and in hydroponic experiments under increasing levels of Cd(2+) (0, 100, 200, 300, 400 and 500 microM in vitro and 0, 50, 100, 150 and 200 microM in hydroponic culture). At 22 and 7 days of exposure to Cd(2+), for the in vitro and hydroponic experiment, respectively, the plantlets were separated into roots and shoot, which were analyzed for biomass as well as Cd(2+), and macro (Ca(2+), K(+) and Mg(2+)) and micronutrient (Cu(2+), Fe(2+), Mn(2+) and Zn(2+)) contents. In the hydroponic experiment, there was no reduction in shoot and root dry weight for any Cd(2+) level, regardless of the potato cultivar. In contrast, in the in vitro experiment, there was an increase in biomass at low Cd(2+) levels, while higher Cd(2+) levels caused a decrease. In general, Cd(2+) decreased the macronutrient and micronutrient contents in the in vitro cultured plantlets in both roots and shoot of cultivars. In contrast, the macronutrient and micronutrient contents in the hydroponically grown plantlets were generally not affected by Cd(2+). Our data suggest that the influence of Cd(2+) on nutrient content in potato was related to the level of Cd(2+) in the substrate, potato cultivar, plant organ, essential element, growth medium and exposure time.
Departamento de Biologia, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, 97105-900 Santa Maria, RS, Brazil.
Jamile Fabbrin
JF
Gonçalves
Fabiane Goldschmidt
FG
Antes
Joseila
J
Maldaner
Luciane Belmonte
LB
Pereira
Luciane Almeri
LA
Tabaldi
Renata
R
Rauber
Liana Veronica
LV
Rossato
Dilson Antônio
DA
Bisognin
Valderi Luiz
VL
Dressler
Erico Marlon de Moraes
EM
Flores
Fernando Teixeira
FT
Nicoloso
D018533:Biomass; D002104:Cadmium / Q000737:chemistry / Q000378:metabolism*; D002118:Calcium / Q000737:chemistry; D004305:Dose-Response Relationship, Drug; D007477:Ions; D008274:Magnesium / Q000737:chemistry; D019216:Metals, Heavy; D018977:Micronutrients / Q000737:chemistry; D008954:Models, Biological; D018521:Plant Physiological Phenomena; D018520:Plant Shoots / Q000378:metabolism; D011188:Potassium / Q000737:chemistry; D011198:Solanum tuberosum / Q000235:genetics* / Q000502:physiology*
D007477:Ions; D019216:Metals, Heavy; D018977:Micronutrients; D002104:Cadmium; D008274:Magnesium; D011188:Potassium; D002118:Calcium