Gene expression changes in a chemoresistant model with human esophageal cancer xenografts using cDNA microarray.
BACKGROUND
5-Fluorouracil (5-FU) and cisplatin combined chemotherapy (FP) is commonly used for esophageal cancer. Acquired resistance needs to be overcome to improve the chemotherapeutic effect.


METHODS
The FP-resistant xenograft model using severe combined immunodeficient (SCID) mice was established as an acquired resistance model. RNA was extracted pretreatment, at the onset of the anticancer effect, during the most effective, and regrowth period in the FP administration group and during the mid-progressive period and the far advanced period in the control group. A microarray was applied to explore gene expression changes.


RESULTS
The data set containing up-regulated genes in the regrowth period was uploaded into Ingenuity Pathway Analysis. The expression change profiles suggested that activation of not only 5-FU- and cisplatin-specific genes, but also the Phosphoinositide 3-kinase (PI3K)/AKT signal were associated with FP resistance.


CONCLUSIONS
A xenograft model using SCID mice with esophageal cancer cells would monitor gene changes during treatment and regrowth.
Yoshida
Takahiro
T
Department of Thoracic, Endocrine Surgery and Oncology, The University of Tokushima Graduate School, 2-50-1, Kuramoto-cho, Tokushima 770-8503, Japan.
Miyoshi
Takanori
T
Seike
Jun-Ichi
J
Yamai
Hiromichi
H
Takechi
Hirokazu
H
Yuasa
Yasuhiro
Y
Tangoku
Akira
A
D000818:Animals; D000971:Antineoplastic Combined Chemotherapy Protocols / Q000627:therapeutic use; D014408:Biomarkers, Tumor / Q000235:genetics / Q000378:metabolism; D002294:Carcinoma, Squamous Cell / Q000188:drug therapy / Q000235:genetics* / Q000378:metabolism; D002945:Cisplatin / Q000008:administration & dosage; D019008:Drug Resistance, Neoplasm; D004938:Esophageal Neoplasms / Q000188:drug therapy / Q000235:genetics* / Q000378:metabolism; D005472:Fluorouracil / Q000008:administration & dosage; D020869:Gene Expression Profiling; D006801:Humans; D051379:Mice; D016513:Mice, SCID; D020411:Oligonucleotide Array Sequence Analysis; D023041:Xenograft Model Antitumor Assays
D014408:Biomarkers, Tumor; D002945:Cisplatin; D005472:Fluorouracil