Allele-specific effects of human deafness gamma-actin mutations (DFNA20/26) on the actin/cofilin interaction.
Auditory hair cell function requires proper assembly and regulation of the nonmuscle gamma isoactin-rich cytoskeleton, and six point mutations in this isoactin cause a type of delayed onset autosomal dominant nonsyndromic progressive hearing loss, DFNA20/26. The molecular basis underlying this actin-dependent hearing loss is unknown. To address this problem, the mutations have been introduced into yeast actin, and their effects on actin function were assessed in vivo and in vitro. Because we previously showed that polymerization was unaffected in five of the six mutants, we have focused on proteins that regulate actin, in particular cofilin, which severs F-actin and sequesters actin monomers. The mutations do not affect the interaction of cofilin with G-actin. However, T89I and V370A mutant F-actins are much more susceptible to cofilin disassembly than WT filaments in vitro. Conversely, P332A filaments demonstrate enhanced resistance. Wild type actin solutions containing T89I, K118M, or P332A mutant actins at mole fractions similar to those found in the hair cell respond in vitro toward cofilin in a manner proportional to the level of the mutant present. Finally, depression of cofilin action in vivo by elimination of the cofilin-activating protein, Aip1p, rescues the inability to grow on glycerol caused by K118M, T278I, P332A, and V370A. These results suggest that a filament instability caused by these mutations can be balanced by decreasing a system in vivo that promotes increased filament turnover. Such mutant-dependent filament destabilization could easily result in hair cell malfunction leading to the late-onset hearing loss observed in these patients.
Department of Biochemistry, University of Iowa Carver College of Medicine, Iowa City, Iowa 52242-1109, USA.
Keith E
KE
Bryan
Peter A
PA
Rubenstein
D008841:Actin Cytoskeleton / Q000378:metabolism; D051339:Actin Depolymerizing Factors / Q000235:genetics* / Q000378:metabolism; D000199:Actins / Q000235:genetics* / Q000378:metabolism; D000244:Adenosine Diphosphate / Q000378:metabolism; D000483:Alleles; D003638:Deafness / Q000235:genetics*; D006801:Humans; D008928:Mitochondria / Q000378:metabolism; D016297:Mutagenesis, Site-Directed; D010641:Phenotype; D011485:Protein Binding; D015003:Yeasts / Q000235:genetics*
D051339:Actin Depolymerizing Factors; D000199:Actins; D000244:Adenosine Diphosphate