Modulation of mitomycin C-induced genotoxicity by acetyl- and thio- analogues of salicylic acid.
BACKGROUND
Recent reports regarding acetylsalicylic acid (ASA) and its metabolites suggest suppressive effects against mitomycin C (MMC)-induced genotoxicity in a mice chromosomal aberration assay. Keeping this in mind, the potential anti-genotoxic effect of the thio-analogue of salicylic acid namely thio-salicylic acid (TSA) was speculated upon. The present study investigated and compared the anti-genotoxic potential of ASA and TSA.


METHODS
The study was performed in male swiss mice (20+/-2 g) using single-cell gel electrophoresis and a peripheral blood micronucleus assay. ASA and TSA (5, 10 or 20 mg/kg) were administered 15 minutes after MMC (1 mg/kg) once daily for 3 or 7 days.


RESULTS
Both ASA and TSA significantly decreased the DNA damage induced by MMC as indicated by a decrease in the comet parameters in bone marrow cells and decreased frequencies of micronucleated reticulocytes in peripheral blood.


CONCLUSIONS
The results clearly demonstrate the anti-genotoxic potential of ASA and TSA.
Department of Pharmacology and Toxicology, National Institute of Pharmaceutical Education and Research, Sector-67, S.A.S. Nagar, Punjab-160062, India.
Amol Ashok
AA
Pawar
Ajit
A
Vikram
Durga Nand
DN
Tripathi
Shweta
S
Padmanabhan
Poduri
P
Ramarao
Gopabandhu
G
Jena
D000818:Animals; D001854:Bone Marrow Cells; D020552:Comet Assay; D004249:DNA Damage; D008297:Male; D051379:Mice; D015162:Micronucleus Tests; D016685:Mitomycin / Q000494:pharmacology*; D009152:Mutagenicity Tests; D009153:Mutagens / Q000633:toxicity; D012156:Reticulocytes / Q000378:metabolism; D020156:Salicylic Acid / Q000737:chemistry / Q000494:pharmacology*; D013997:Time Factors
D009153:Mutagens; D016685:Mitomycin; D020156:Salicylic Acid