Novel fluorescent probe for analysis of hydroperoxides based on boron dipyrromethane fluorophore.
A new reagent, N-[2-(diphenylphosphino)ethyl]-4-(1,3,5,7-tetramethyl-4,4-difluoro-4-bora-3a,4a-diaza-s-indacene-8-yl)benzamide (DPPEA-BODIPY), was designed and synthesized for analysis of hydroperoxides. DPPEA-BODIPY fluoresces at low levels in the visible range (lambda(ex)/lambda(em) = 502 nm/515 nm) and reacts with hydroperoxides to produce DPPEA-BODIPY oxide, which fluoresces at high levels. The fluorescence intensity of the reaction mixture was observed to be linearly related to the methyl linoleate hydroperoxide (MeLOOH) concentration.
Inoue
Naoko
N
Research Center of Advanced Bionics, National Institute of Advanced Industrial Science and Technology (AIST), Katayanagi Institute, Tokyo University of Technology, Tokyo, Japan. suzuki-yoshio@aist.go.jp
Suzuki
Yoshio
Y
Yokoyama
Kenji
K
Karube
Isao
I
D001896:Boron Compounds / Q000138:chemical synthesis* / Q000737:chemistry; D004579:Electron Transport; D005456:Fluorescent Dyes / Q000138:chemical synthesis* / Q000737:chemistry; D006861:Hydrogen Peroxide / Q000032:analysis* / Q000737:chemistry; D008055:Lipids / Q000737:chemistry; D010720:Phosphines / Q000737:chemistry
D001896:Boron Compounds; D005456:Fluorescent Dyes; D008055:Lipids; C543109:N-(2-(diphenylphosphino)ethyl)-4-(1,3,5,7-tetramethyl-4,4-difluoro-4-bora-3a,4a-diaza-s-indacene-8-yl)benzamide; D010720:Phosphines; D006861:Hydrogen Peroxide; C044646:phosphine