Oxidative damage in dengue fever.
Oxidative stress may be important in the pathogenesis of dengue infection. Using accurate markers of oxidative damage, we assessed the extent of oxidative damage in dengue patients. The levels of hydroxyeicosatetraenoic acid products (HETEs), F(2)-isoprostanes (F(2)-IsoPs), and cholesterol oxidation products (COPs) were measured in 28 adult dengue patients and 28 age-matched study controls during the febrile, defervescent, and convalescent stages of infection. We compared the absolute and the percentage change in these markers in relation to key clinical parameters and inflammatory markers. The levels of total HETEs and total HETEs/arachidonate, total F(2)-IsoPs/arachidonate, and COPs/cholesterol were higher during the febrile compared to the convalescent level. Total HETEs correlated positively with admission systolic blood pressure (r=0.52, p<0.05), whereas an inverse relationship was found between 7beta-hydroxycholesterol and systolic and diastolic blood pressure (r=-0.61 and -0.59, respectively, p<0.01). The urinary F(2)-IsoP level was higher in urine during the febrile stage compared to the convalescent level. Despite lower total cholesterol levels during the febrile stage compared to convalescent levels, a higher percentage of cholesterol was found as COPs (7beta-, 24-, and 27-hydroxycholesterol). The levels of platelet-activating factor-acetylhydrolase activity, vascular cellular adhesion molecule-1, tumor necrosis factor-alpha, and high-sensitivity C-reactive protein were higher during the febrile stage compared to their convalescent levels (p<0.01). Markers of oxidative damage are altered during the various stages of dengue infection.
Department of Medicine, Yong Loo Lin School of Medicine, National University Hospital, Singapore 119074. raymond_seet@nus.edu.sg
Raymond C S
RC
Seet
Chung-Yung J
CY
Lee
Erle C H
EC
Lim
Amy M L
AM
Quek
Leonard L L
LL
Yeo
Shan-Hong
SH
Huang
Barry
B
Halliwell
D043203:1-Alkyl-2-acetylglycerophosphocholine Esterase / Q000235:genetics / Q000378:metabolism; D000328:Adult; D000368:Aged; D015415:Biomarkers / Q000097:blood* / Q000652:urine; D001794:Blood Pressure; D002097:C-Reactive Protein / Q000235:genetics / Q000378:metabolism; D002784:Cholesterol / Q000031:analogs & derivatives / Q000097:blood* / Q000652:urine; D003715:Dengue / Q000097:blood / Q000235:genetics / Q000503:physiopathology*; D003716:Dengue Virus / Q000472:pathogenicity / Q000502:physiology*; D018450:Disease Progression; D028441:F2-Isoprostanes / Q000097:blood* / Q000652:urine; D005260:Female; D005786:Gene Expression Regulation; D006801:Humans; D006893:Hydroxyeicosatetraenoic Acids / Q000097:blood* / Q000652:urine; D008297:Male; D008875:Middle Aged; D018384:Oxidative Stress; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000378:metabolism; D014774:Virulence
D015415:Biomarkers; D028441:F2-Isoprostanes; D006893:Hydroxyeicosatetraenoic Acids; D014409:Tumor Necrosis Factor-alpha; D002097:C-Reactive Protein; D002784:Cholesterol; D043203:1-Alkyl-2-acetylglycerophosphocholine Esterase