Preventive effects of salvianolic acid B on transforming growth factor-beta1-induced epithelial-to-mesenchymal transition of human kidney cells.
Epithelial-mesenchymal transition (EMT) is an important mechanism in kidney fibrosis. While Salvianolic acid-B (Sal B) has been well appreciated to show a protective effect of tissue fibrosis, the objective of this study was to investigate the influence of Sal B on the transdifferentiation of renal tubular epithelial cells. Human kidney proximal tubular cell line (HK-2) was used as the proximal tubular cell model and EMT was induced with 5 ng/ml of human transforming growth factor (TGF)-beta1. The effects of the Sal B on cell morphology were observed by phase contrast microscopy, and the possible mechanisms were studied by immunocytochemistry and real-time reverse transcription-polymerase chain reaction. Our results revealed that Sal B could inhibit TGF-beta1-induced myofibroblast phenotype and restored the epithelial morphology in a dose-dependent manner. It was partially through modulating alpha-smooth muscle actin (SMA) increase and E-cadherin reduction. These observations strongly suggest that Sal B is a potent inhibitor of TGF-beta1-induced EMT and might be a promising agent for treating tubulointerstitial fibrosis.
Yao
Gang
G
Department of Nephrology, 2nd Affiliated Hospital of Nanjing Medical University, Nanjing, P.R. China. yg921@163.com
Xu
Lizhi
L
Wu
Xiaochun
X
Xu
Lingling
L
Yang
Junwei
J
Chen
Huimei
H
D001572:Benzofurans / Q000494:pharmacology*; D002460:Cell Line; D054338:Cell Transdifferentiation / Q000187:drug effects*; D004847:Epithelial Cells / Q000187:drug effects* / Q000473:pathology; D005355:Fibrosis; D006801:Humans; D007150:Immunohistochemistry; D007687:Kidney Tubules, Proximal / Q000187:drug effects* / Q000473:pathology; D008648:Mesoderm / Q000187:drug effects* / Q000473:pathology; D009395:Nephritis, Interstitial / Q000473:pathology / Q000517:prevention & control; D020011:Protective Agents / Q000494:pharmacology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D053773:Transforming Growth Factor beta1 / Q000494:pharmacology*; D053630:Depsides
D001572:Benzofurans; D020011:Protective Agents; D053773:Transforming Growth Factor beta1; C076944:salvianolic acid B; D053630:Depsides