Rapid gynogenetic mapping of Xenopus tropicalis mutations to chromosomes.
Pilot forward genetic screens in Xenopus tropicalis have isolated over 60 recessive mutations. Here we present a simple method for mapping mutations to chromosomes using gynogenesis and centromeric markers. When coupled with available genomic resources, gross mapping facilitates evaluation of candidate genes as well as higher resolution linkage studies. Using gynogenesis, we have mapped the genetic locations of the 10 X. tropicalis centromeres, and performed fluorescence in situ hybridization to validate these locations cytologically. We demonstrate the use of this very small set of centromeric markers to map mutations efficiently to specific chromosomes. Developmental Dynamics 238:1398-1406, 2009. (c) 2009 Wiley-Liss, Inc.
Khokha
Mustafa K
MK
Department of Pediatrics and Genetics, Yale University School of Medicine, New Haven, Connecticut, USA.
Krylov
Vladimir
V
Reilly
Michael J
MJ
Gall
Joseph G
JG
Bhattacharya
Dipankan
D
Cheung
Chung Yan J
CY
Kaufman
Sarah
S
Lam
Dang Khoa
DK
Macha
Jaroslav
J
Ngo
Catherine
C
Prakash
Neha
N
Schmidt
Philip
P
Tlapakova
Tereza
T
Trivedi
Toral
T
Tumova
Lucie
L
Abu-Daya
Anita
A
Geach
Timothy
T
Vendrell
Elisenda
E
Ironfield
Holly
H
Sinzelle
Ludivine
L
Sater
Amy K
AK
Wells
Dan E
DE
Harland
Richard M
RM
Zimmerman
Lyle B
LB
D000818:Animals; D002503:Centromere / Q000235:genetics; D002874:Chromosome Mapping / Q000379:methods*; D002875:Chromosomes; D005260:Female; D005819:Genetic Markers; D008297:Male; D008540:Meiosis / Q000502:physiology; D009154:Mutation; D011995:Recombination, Genetic; D014981:Xenopus / Q000235:genetics*
D005819:Genetic Markers