Phenolic acids determination by HPLC-DAD-ESI/MS in sixteen different Portuguese wild mushrooms species.
Analysis of phenolic compounds in sixteen Portuguese wild mushrooms species has been carried out by high-performance liquid chromatography coupled to photodiode array detector and mass spectrometer (HPLC-DAD-ESI/MS). No flavonoids were detected in the analysed samples, but diverse phenolic acids namely protocatechuic, p-hydroxybenzoic and p-coumaric acids, and two vanillic acid isomers were found and quantified. A related non-phenolic compound, cinnamic acid, was also detected in some samples, being the only compound found in Cantharellus cibarius (14.97 mg/kg, dry matter), Lycoperdon perlatum (14.36 mg/kg) and Macrolepiota procera (21.53 mg/kg). p-Hydroxybenzoic acid was found in the majority of the samples, being the most abundant compound in Agaricus silvicola (238.7 mg/kg). Ramaria botrytis showed the highest phenolic acids concentration (356.7 mg/kg) due to the significant contribution of protocatechuic acid (342.7 mg/kg).
Unidad de Nutrición y Bromatología, Facultad de Farmacia, Universidad de Salamanca, Campus Miguel de Unamuno, 37007 Salamanca, Spain.
Lillian
L
Barros
Montserrat
M
Dueñas
Isabel C F R
IC
Ferreira
Paula
P
Baptista
Celestino
C
Santos-Buelga
D000363:Agaricales / Q000737:chemistry*; D001713:Biphenyl Compounds; D002851:Chromatography, High Pressure Liquid; D016166:Free Radical Scavengers / Q000494:pharmacology; D062385:Hydroxybenzoates / Q000032:analysis* / Q000494:pharmacology; D007202:Indicators and Reagents; D010851:Picrates; D011174:Portugal; D012015:Reference Standards; D021241:Spectrometry, Mass, Electrospray Ionization
D001713:Biphenyl Compounds; D016166:Free Radical Scavengers; D062385:Hydroxybenzoates; D007202:Indicators and Reagents; D010851:Picrates; C004931:1,1-diphenyl-2-picrylhydrazyl; C017616:phenolic acid