Xenotransplantation by injection of a suspension of isolated preantral ovarian follicles and stroma cells under the kidney capsule of nude mice.
OBJECTIVE
To develop and test a novel approach to xenotransplantation of isolated preantral follicles underneath the kidney capsule of immunodeficient mice.


METHODS
Prospective experimental animal study.


METHODS
Academic research unit.


METHODS
Healthy adult nude mice.


METHODS
Bovine ovaries from fetuses (n = 3) and calves (n = 3) were enzymatically disaggregated and subsequently filtered. Isolated preantral follicles were suspended in phosphate buffered saline, and granulosa and stroma cells originating from the ovarian digest served as embedding matrix. The suspension was injected under the kidney capsule of adult nude mice.


METHODS
Fourteen days after transplantation, follicular survival and proliferation in grafts was assessed by histology and proliferating cell nuclear antigen (PCNA) immunostaining, and was compared with ungrafted control tissue.


RESULTS
Primordial follicles decreased from 58.2% in control tissue to 17.1% in transplants in the fetal group, and from 76.0% to 17.2% in the calf group. Concomitantly, primary follicles increased from 13.4% to 62.2% in the fetal group, and from 5.4% to 63.5% in the calf group. Follicular proliferation measured by PCNA immunolabeling exhibited an increase from 40.6% growing follicles to 81.9% in the fetal group, and from 21.0% to 80.7% in the calf group.


CONCLUSIONS
The massive follicular activation following transplantation indicates that isolated preantral follicles are able to survive and grow 14 days after renal subcapsular xenotransplantation.
Department of Veterinary Medicine, Faculty of Pharmaceutical, Biomedical, and Veterinary Sciences, University of Antwerp, Belgium. jan.aerts@ua.ac.be
Jan M J
JM
Aerts
Belen
B
Martinez-Madrid
Jo L M R
JL
Leroy
Stefan
S
Van Aelst
Peter E J
PE
Bols
D000818:Animals; D002417:Cattle; D002455:Cell Division; D005260:Female; D006085:Graft Survival; D006107:Granulosa Cells / Q000637:transplantation; D007150:Immunohistochemistry; D007267:Injections; D007668:Kidney; D051379:Mice; D008819:Mice, Nude; D006080:Ovarian Follicle / Q000166:cytology / Q000378:metabolism / Q000637:transplantation*; D011247:Pregnancy; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism; D017154:Stromal Cells / Q000637:transplantation*; D014183:Transplantation, Heterologous / Q000379:methods*
D018809:Proliferating Cell Nuclear Antigen