Induction of apoptosis by beta-carotene and dimethyl tetrasulfide assisted by UVA irradiation in HL-60 cells.
Cellular phototoxicity induced by UVA irradiation and its potential application to therapy has been reported. In the present study, the induction of apoptosis induced by beta-carotene and dimethyl tetrasulfide (Me(2)S(4)) assisted by UVA irradiation in HL-60 cells was assessed. beta-carotene assisted by UVA significantly decreased the cell viability and induced DNA fragmentation in HL-60 cells. Me(2)S(4) combined with beta-carotene and assisted by UVA significantly inhibited the cell viability, and enhanced the caspase-3 activity which was completely inhibited by N-acety-L-cysteine. beta-carotene was significantly degraded by UVA, but this was not accelerated by Me(2)S(4) in a cell culture system. The photodegradation products of beta-carotene prepared by UVA irradiation regardless of the addition of Me(2)S(4) showed lower cytotoxicity than beta-carotene itself in HL-60 cells. These results suggest that the ROS- and caspase-3-dependent apoptosis induced by beta-carotene and Me(2)S(4) assisted by UVA was due to a synergistic action rather than to the sole effect of the photodegradation products of beta-carotene in HL-60 cells.
Graduate School of Natural Science and Technology, Okayama University, Okayama, Japan.
Gongliang
G
Zhang
Haitao
H
Wu
Beiwei
B
Zhu
Yasuaki
Y
Shimoishi
Yoshimasa
Y
Nakamura
Yoshiyuki
Y
Murata
D000111:Acetylcysteine / Q000494:pharmacology; D000970:Antineoplastic Agents / Q000737:chemistry / Q000494:pharmacology*; D000975:Antioxidants / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects* / Q000528:radiation effects*; D053148:Caspase 3 / Q000378:metabolism; D049109:Cell Proliferation / Q000187:drug effects / Q000528:radiation effects; D002470:Cell Survival / Q000187:drug effects / Q000528:radiation effects; D004789:Enzyme Activation / Q000187:drug effects / Q000528:radiation effects; D018922:HL-60 Cells; D006801:Humans; D019169:Jurkat Cells; D010782:Photolysis / Q000187:drug effects; D017382:Reactive Oxygen Species / Q000378:metabolism; D013440:Sulfides / Q000494:pharmacology*; D014466:Ultraviolet Rays; D019207:beta Carotene / Q000737:chemistry / Q000494:pharmacology*
D000970:Antineoplastic Agents; D000975:Antioxidants; D017382:Reactive Oxygen Species; D013440:Sulfides; C532382:dimethyl tetrasulfide; D019207:beta Carotene; D053148:Caspase 3; D000111:Acetylcysteine