Smart conferring of nuclease resistance to DNA by 3'-end protection using 2',4'-bridged nucleoside-5'-triphosphates.
Incorporation of 2',4'-bridged nucleotides into the 3'-end of oligodeoxyribonucleotide (ODN) was examined using terminal deoxynucleotidyl transferase (TdT). The three types of 2',4'-bridged nucleoside-5'-triphospates with different bridging structures used were incorporated efficiently into the 3'-end of DNA by TdT, although only single nucleotide incorporation was observed. Nuclease resistance was conferred on DNA, depending on the types of bridging nucleotides added.
Kuwahara
Masayasu
M
Department of Chemistry and Chemical Biology, Graduate School of Engineering, Gunma University, Gunma, Japan. kuwahara@chem-bio.gunma-u.ac.jp
Obika
Satoshi
S
Takeshima
Hidetoshi
H
Hagiwara
Yoshihiko
Y
Nagashima
Jun-Ichi
J
Ozaki
Hiroaki
H
Sawai
Hiroaki
H
Imanishi
Takeshi
T
D004247:DNA / Q000737:chemistry / Q000378:metabolism*; D004253:DNA Nucleotidylexotransferase / Q000378:metabolism; D003851:Deoxyribonucleases / Q000378:metabolism*; D009711:Nucleotides / Q000737:chemistry*; D009838:Oligodeoxyribonucleotides / Q000737:chemistry / Q000378:metabolism*
C541966:KTP compound; C541967:LTP compound; C541968:MTP compound; D009711:Nucleotides; D009838:Oligodeoxyribonucleotides; D004247:DNA; D004253:DNA Nucleotidylexotransferase; D003851:Deoxyribonucleases