Semicontinuous flow electroporation chip for high-throughput transfection on mammalian cells.
We have recently developed a semicontinuous flow electroporation (SFE) device for in vitro DNA delivery. Cells mixed with plasmid DNA continuously flowed through a serpentine channel, the side walls of which also serving as electrodes. With the use of pWizGFP plasmid and K562 cells as a model system, SFE showed better transgene expression (10-15%) compared to a commercial electroporation system. Quantitative results via MTS assay also revealed a 50% or higher cell viability. Similar observations were also found with pWizGFP transfection to mouse embryonic stem cells. Such improvements were attributed to less gas formation and Joule heating in SFE.
Wang
Shengnian
S
Department of Chemical and Biomolecular Engineering, NSF Center for Affordable Nanoengineering of Polymer Biomedical Devices, The Ohio State University, 140 West 19th Avenue, Columbus, Ohio 43210, USA.
Zhang
Xulang
X
Wang
Weixiong
W
Lee
L James
LJ
D000818:Animals; D045744:Cell Line, Tumor; D002470:Cell Survival; D004566:Electrodes; D018274:Electroporation / Q000295:instrumentation*; D004622:Embryo, Mammalian / Q000166:cytology; D004867:Equipment Design; D018014:Gene Transfer Techniques / Q000295:instrumentation*; D006801:Humans; D051379:Mice; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D010957:Plasmids / Q000235:genetics; D013234:Stem Cells / Q000166:cytology; D013696:Temperature; D014162:Transfection