FAT10 level in human gastric cancer and its relation with mutant p53 level, lymph node metastasis and TNM staging.
OBJECTIVE
To investigate the role of FAT10 and mutant p53 in the pathogenesis, severity and prognosis of gastric cancer.


METHODS
FAT10, mutant p53 mRNA and protein levels were measured by reverse transcription (RT)-PCR and immunohistochemistry in gastric cancer tissue (n = 62), tumor-adjacent tissue (n = 62) and normal gastric tissue (n = 62). Relation of FAT10 and mutant p53 expression with clinicopathological features and clinical outcomes of gastric cancer patients were analyzed.


RESULTS
The FAT10, mutant p53 mRNA and protein levels were significantly higher in gastric cancer than in its adjacent and normal tissue. The FAT10 and mutant p53 levels in gastric cancer tissue were significantly correlated with lymph node metastasis and tumor, nodes, metastasis (TNM) staging. Moreover, the high FAT10 level was associated with the overall survival rate of patients. Multivariate Cox-proportional hazards model analysis showed that mRNA and protein levels of FAT10 and mutant p53, lymph node metastasis, distant metastasis and TNM stage were the independent prognostic factors for gastric cancer.


CONCLUSIONS
FAT10 may be involved in gastric carcinogenesis, and is a potential marker for the prognosis of gastric cancer patients. FAT10 and mutant p53 may play a common role in the carcinogenesis of gastric cancer.
Department of Digestive Diseases, First Affiliated Hospital of Zhejiang University Medical School, No. 79 Qingchun Road, Hangzhou 310003, Zhejiang Province, China. jifeng1126@sina.com
Feng
F
Ji
Xi
X
Jin
Chun-Hua
CH
Jiao
Qin-Wei
QW
Xu
Zi-Wei
ZW
Wang
Yue-Liang
YL
Chen
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000378:metabolism; D005260:Female; D006801:Humans; D008207:Lymphatic Metastasis / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D011379:Prognosis; D013274:Stomach Neoplasms / Q000175:diagnosis / Q000378:metabolism / Q000473:pathology; D015996:Survival Rate; D016159:Tumor Suppressor Protein p53 / Q000235:genetics / Q000378:metabolism; D014452:Ubiquitins / Q000235:genetics / Q000378:metabolism*; D055815:Young Adult
D014408:Biomarkers, Tumor; D016159:Tumor Suppressor Protein p53; C109184:UBD protein, human; D014452:Ubiquitins