High expression of WT1 gene in acute myeloid leukemias with more predominant WT1+17AA isoforms at relapse.
Real-time quantitative reverse transcriptase polymerase chain reaction method was established for detecting the expression levels of WT1 gene and WT1+17AA isoforms in 226 acute myeloid leukemia (AML) bone marrow (BM) cells. The results showed that WT1 gene was 2-3 logarithms expressed more in AML BM cells at initial diagnosis or relapse than in normal BM cells (p<0.001), with predominant WT1+17AA isoforms expression (the ratio of WT1+17AA/WT1 more than 0.50). Interestingly the ratio of WT1+17AA/WT1 was statistically higher in relapsed AMLs than in initially diagnosed (p=0.01), speculating that WT1+17AA isoforms might participate in AML relapse.
Department of Hematology, The First People's Hospital of Changzhou, Third Affiliated to Suzhou University, Changzhou, China. guweiying2001@163.com
Weiying
W
Gu
Shaoyan
S
Hu
Zixing
Z
Chen
Guoqiang
G
Qiu
Jiannong
J
Cen
Bai
B
He
Jun
J
He
Wei
W
Wu
D001483:Base Sequence; D017931:DNA Primers; D016162:Genes, Wilms Tumor; D006801:Humans; D015470:Leukemia, Myeloid, Acute / Q000235:genetics* / Q000473:pathology; D012008:Recurrence; D020133:Reverse Transcriptase Polymerase Chain Reaction
D017931:DNA Primers