Chitosan-graft-beta-cyclodextrin scaffolds with controlled drug release capability for tissue engineering applications.
Biodegradable scaffolds composed of chitosan-g-beta-cyclodextrin (chit-g-beta-CD) were prepared by freeze-drying method as synthetic extracellular matrices to fill the gap during the healing process. Due to the presence of beta-CD, these scaffolds can be used as a matrix for drug loading and controlled release. The morphology, swelling and drug release properties of the scaffolds were found to be dependent on the extent of cross-linking density in the scaffolds. The drug dissolution profile showed that chit-g-beta-CD scaffolds provided a slower release of the entrapped ketoprofen than chitosan scaffold. The MTT assay showed that there is no obvious cytotoxicity of chit-g-beta-CD scaffolds cross-linked with 0.01 M of glutaraldehyde against the fibroblasts (L929) cells. These results suggest that chit-g-beta-CD scaffolds may become a potential biodegradable active filling material with controlled drug release capability, which provide a healthy environment and enhance the surrounding tissue regeneration.
Department of Product Development, Taiwan Textile Research Institute, Taipei County -23674, Taiwan. mprabaharan@yahoo.com
M
M
Prabaharan
R
R
Jayakumar
D000818:Animals; D002234:Carbodiimides / Q000737:chemistry; D002460:Cell Line; D048271:Chitosan / Q000378:metabolism*; D016503:Drug Delivery Systems; D005347:Fibroblasts / Q000187:drug effects; D006863:Hydrogen-Ion Concentration; D057927:Hydrophobic and Hydrophilic Interactions; D007660:Ketoprofen / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D004364:Pharmaceutical Preparations / Q000378:metabolism*; D017550:Spectroscopy, Fourier Transform Infrared; D013696:Temperature; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry*; D014867:Water / Q000737:chemistry; D047392:beta-Cyclodextrins / Q000378:metabolism*
D002234:Carbodiimides; D004364:Pharmaceutical Preparations; D047392:beta-Cyclodextrins; D014867:Water; D048271:Chitosan; D007660:Ketoprofen; C031215:betadex