Identification of proteins interacting with selenocysteine lyase.
Yeast two-hybrid screening of mouse cDNA libraries was performed to identify proteins interacting with selenocysteine lyase (SCL), which decomposes L-selenocysteine. Several proteins related to spermatogenesis, protein synthesis, and cell viability/apoptosis were identified as potential interactors. Major urinary proteins 1 and 2 interacted with SCL and inhibited its activity. Coimmunoprecipitation revealed interactions between SCL and each of two selenophosphate synthetase isozymes.
Tobe
Ryuta
R
Institute for Chemical Research, Kyoto University, Kyoto, Japan.
Mihara
Hisaaki
H
Kurihara
Tatsuo
T
Esaki
Nobuyoshi
N
D004791:Enzyme Inhibitors / Q000032:analysis / Q000378:metabolism / Q000494:pharmacology; D007527:Isoenzymes / Q000037:antagonists & inhibitors / Q000378:metabolism; D008190:Lyases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D011506:Proteins / Q000032:analysis* / Q000378:metabolism* / Q000494:pharmacology; D020798:Two-Hybrid System Techniques
D004791:Enzyme Inhibitors; D007527:Isoenzymes; D011506:Proteins; C035737:major urinary proteins; D008190:Lyases; C034278:selenocysteine lyase