Ventromedial hypothalamic lesions change the expression of neuron-related genes and immune-related genes in rat liver.
There are no reports that hypothalamus can directly affect the expression of neuron-related genes and immune-related genes in liver. We identified genes of which expression profiles showed significant modulation in rat liver after ventromedial hypothalamic (VMH) lesions. Total RNA was extracted, and differences in the gene expression profiles between rats at day 3 after VMH lesioning and sham-VMH lesioned rats were investigated using DNA microarray analysis. The result revealed that VMH lesions regulated the genes that were involved in functions related to neuronal development and immunofunction in the liver. Real-time PCR also confirmed that gene expression of SULT4A1 was upregulated, but expression of ACSL1 and CISH were downregulated at day 3 after VMH lesions. VMH lesions may change the expression of neuron-related genes and immune-related genes in rat liver.
Laboratory of Clinical Nutrition & Physiology, Kyoritsu Women's University, 2-2-1 Hitotsubashi Chiyoda-ku, Tokyo 101-8433, Japan.
Takayoshi
T
Kiba
Yuri
Y
Kintaka
Yoko
Y
Suzuki
Eiko
E
Nakata
Yasuhito
Y
Ishigaki
Shuji
S
Inoue
D000818:Animals; D051792:Basic Helix-Loop-Helix Proteins / Q000096:biosynthesis / Q000235:genetics; D005260:Female; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D007031:Hypothalamus / Q000166:cytology / Q000502:physiology*; D008099:Liver / Q000276:immunology* / Q000294:innervation / Q000378:metabolism*; D015213:Neuroimmunomodulation; D009474:Neurons / Q000502:physiology*; D020411:Oligonucleotide Array Sequence Analysis; D016133:Polymerase Chain Reaction; D051381:Rats; D017207:Rats, Sprague-Dawley; D015238:Sulfotransferases / Q000096:biosynthesis / Q000235:genetics; D050826:Suppressor of Cytokine Signaling Proteins / Q000096:biosynthesis / Q000235:genetics
D051792:Basic Helix-Loop-Helix Proteins; D015238:Sulfotransferases; D050826:Suppressor of Cytokine Signaling Proteins; C499318:Ascl1 protein, rat