Phylogenetic analysis of the RNA-dependent RNA polymerase (RdRp) and a predicted structural protein (pSP) of the Chronic bee paralysis virus (CBPV) isolated from various geographic regions.
Chronic bee paralysis virus (CBPV) is responsible for chronic paralysis, an infectious and contagious disease of adult honey bees (Apis mellifera L.). The full-length nucleotide sequences of the two major RNAs of CBPV have previously been characterized. The Orf3 of RNA1 has shown significant similarities to the RNA-dependent RNA polymerase (RdRp) of positive single-stranded RNA viruses, whereas the Orf3 of RNA2 encodes a putative structural protein (pSP). In the present study, honey bees originating from 9 different countries (Austria, Poland, Hungary, Spain, Belgium, Denmark, Switzerland, Uruguay and France) were analysed for the presence of CBPV genome. The complete genomic nucleotide sequence of the RdRp (1947bp) and of the pSP (543bp) from 24 honey bee positive samples was determined and the phylogenetic relationship among isolates was investigated. Four distinct genotypes of CBPV were observed.
Agence Française de Sécurité Sanitaire des Aliments (AFSSA), Les Templiers, Route des Chappes, BP 111, 06902 Sophia Antipolis, France. p.blanchard@afssa.fr
Philippe
P
Blanchard
Frank
F
Schurr
Violaine
V
Olivier
Olivier
O
Celle
Karina
K
Antùnez
Tamàs
T
Bakonyi
Hélène
H
Berthoud
Eric
E
Haubruge
Mariano
M
Higes
Sylwia
S
Kasprzak
Hemma
H
Koeglberger
Per
P
Kryger
Richard
R
Thiéry
Magali
M
Ribière
D000818:Animals; D001317:Austria; D001516:Bees / Q000821:virology*; D001530:Belgium; D016000:Cluster Analysis; D003718:Denmark; D005602:France; D005843:Geography; D006814:Hungary; D017720:Molecular Epidemiology; D008969:Molecular Sequence Data; D010802:Phylogeny; D011044:Poland; D012324:RNA-Dependent RNA Polymerase / Q000235:genetics*; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D013030:Spain; D013557:Switzerland; D014583:Uruguay; D014764:Viral Proteins / Q000235:genetics*; D015678:Viral Structural Proteins / Q000235:genetics*; D014713:Viruses, Unclassified / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification
D014764:Viral Proteins; D015678:Viral Structural Proteins; D012324:RNA-Dependent RNA Polymerase