Pro-apoptotic activity and mono-/diubiquitylation of Xenopus Bid in egg extracts.
Apoptosis in Xenopus egg extracts is carried out by maternally stockpiled materials, but the contributions of endogenous apoptosis regulators are still poorly characterized. Here we examined the physiological role of Xenopus Bid (xBid), a pro-apoptotic BH3-only member of Bcl-2 family proteins. We found that endogenous xBid was a physiological accelerator of apoptosis in egg extracts. Interestingly, xBid was mono-/diubiquitylated but not degraded by proteasome in egg extracts, and we identified three ubiquitylated Lys residues in the N-terminal propeptide region. Comparison with human Bid suggested that mono-/diubiquitylation is a specific feature of xBid.
Department of Obstetrics and Gynecology, Toho University Medical Center Sakura Hospital, 564-1 Shimoshizu, Sakura-shi, Chiba 285-8741, Japan.
Tomohiro
T
Saitoh
Yuichi
Y
Tsuchiya
Toshihiko
T
Kinoshita
Motohiro
M
Itoh
Shigeru
S
Yamashita
D000818:Animals; D017209:Apoptosis; D051017:Apoptosis Regulatory Proteins / Q000235:genetics* / Q000378:metabolism; D051023:BH3 Interacting Domain Death Agonist Protein / Q000235:genetics / Q000378:metabolism*; D002457:Cell Extracts; D006801:Humans; D009865:Oocytes / Q000378:metabolism; D017434:Protein Structure, Tertiary; D054875:Ubiquitination; D029867:Xenopus Proteins / Q000235:genetics* / Q000378:metabolism; D014982:Xenopus laevis
D051017:Apoptosis Regulatory Proteins; D051023:BH3 Interacting Domain Death Agonist Protein; C541383:Bid protein, Xenopus; D002457:Cell Extracts; D029867:Xenopus Proteins