Determination of oxidative stress in vitiligo by measuring superoxide dismutase and catalase levels in vitiliginous and non-vitiliginous skin.
BACKGROUND
Vitiligo is an acquired disorder characterized by circumscribed depigmented macules devoid of identifiable melanocytes. Complex genetic, immunological, neural and self destructive mechanisms interplay in its pathogenesis. According to autocytotoxic hypothesis, oxidative stress has been suggested to be the initial pathogenic event in melanocyte degeneration.


OBJECTIVE
The aim of our investigation was to evaluate the role of oxidative stress by measuring levels of the antioxidant enzymes superoxide dismutase (SOD) and catalase (CAT) in lesional and normal skin of patients with vitiligo and in the skin of normal controls.


METHODS
We determined the activity of SOD in lesional and non-lesional skin and CAT in lesional skin only of 25 vitiligo patients and 25 controls by using the spectrophotometric assay and Aebi's method, respectively.


RESULTS
There was statistically significant increase in the levels of SOD in vitiliginous and non vitiliginous skin of patient group compared to the control group (P < 0.001). No significant difference was found between the levels of SOD in lesional skin and non-lesional skin of vitiligo patients. The levels of CAT in the skin of patients were found to be significantly lower than those of controls (P < 0.001).


CONCLUSIONS
There is increased oxidative stress in vitiligo as is indicated by high levels of SOD and low levels of CAT in the skin of vitiligo patients.
Department of Dermatology, King George Hospital, Andhra University, Visakhapatnam, AP, India.
P V
PV
Sravani
N Kishore
NK
Babu
K V T
KV
Gopal
G Raghu Rama
GR
Rao
Athota Rama
AR
Rao
Bhagavatula
B
Moorthy
T Raghava
TR
Rao
D000293:Adolescent; D000328:Adult; D002374:Catalase / Q000032:analysis*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D018384:Oxidative Stress / Q000502:physiology*; D012867:Skin / Q000201:enzymology* / Q000473:pathology; D013056:Spectrophotometry, Ultraviolet / Q000379:methods; D013482:Superoxide Dismutase / Q000032:analysis*; D014820:Vitiligo / Q000175:diagnosis* / Q000201:enzymology*; D055815:Young Adult
D002374:Catalase; D013482:Superoxide Dismutase