Analysis and purification of O-decanoyl sucrose regio-isomers by reversed phase high pressure liquid chromatography with evaporative light scattering detection.
Purification of seven regio-isomers of O-decanoyl sucrose, 2-O-, 3-O-, 4-O-, 6-O-, 3'-O-, 4'-O- and 6'-O-decanoyl sucrose, were performed by LC followed by preparative RP-HPLC with ELSD. Using an optimized gradient of acetonitrile in water 2-O-, 3-O-, 6-O- and 3'-O-decanoyl sucrose were purified in yields (w/w) of 52.5%, 34.7%, 45.0% and 36.9%, respectively. In the purified preparations of the 2-O- and 3'-O-decanoyl sucrose respectively, acyl migration was observed as a result of the drying process. Lyophilization resulted in the highest purities (w/w) of 96% and 100% for the 2-O- and the 3'-O-decanoyl sucrose, respectively.
Department of Biotechnology, Chemistry and Environmental Engineering, Aalborg University, Aalborg, Denmark.
Sinthuwat
S
Ritthitham
Reinhard
R
Wimmer
Allan
A
Stensballe
Lars Haastrup
LH
Pedersen
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005612:Freeze Drying; D008027:Light; D012542:Scattering, Radiation; D021241:Spectrometry, Mass, Electrospray Ionization; D013237:Stereoisomerism; D013395:Sucrose / Q000031:analogs & derivatives* / Q000737:chemistry / Q000302:isolation & purification
D013395:Sucrose