[The expression of interferon-alpha in peripheral blood of patients with primary Sjogren's syndrome].
OBJECTIVE
To investigate the interferon-alpha level in peripheral blood of patients with primary Sjogren's syndrome (pSS), and explore preliminarily the correlation between pSS and interferon-alpha.


METHODS
ELISA and real-time PCR were used to evaluate the interferon-alpha level in peripheral blood in 37 patients with pSS and 24 patients with non-pSS. Statistical analysis was performed by Student's t test or Fisher's exact test using SAS6.12 software package.


RESULTS
The results demonstrated that 43.24% (16/37) patients with pSS had detectable IFN-alpha concentration in plasma (>or=12.5 pg/mL), the other 21 patients (56.76%) with pSS had undetectable IFN-alpha concentration(<12.5 pg/mL). All 24 controls had undetectable IFN-alpha expression.There was significant difference between the two groups(P=7.02 x 10(-5)). Furthermore, the mRNA expression levels of IFN-alpha gene in peripheral blood cells were over-expressed in patients with pSS(P=0.0124).


CONCLUSIONS
The study reveals that interferon-alpha level in peripheral blood with pSS patients is overexpressed.
Zheng
Ling-Yan
LY
Department of Oral and Maxillofacial Surgery, Ninth People's Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine, Shanghai Key Laboratory of Stomatology, Shanghai 200011, China. zhenglingyan73@163.com
Zhang
Zhi-Yuan
ZY
Yu
Chuang-Qi
CQ
Yang
Chi
C
Chen
Xiao-Qing
XQ
Zhuang
Xing
X
Tang
Min-Jun
MJ
Wu
Da-Ming
DM
Zhu
Min-Wen
MW
D016022:Case-Control Studies; D006801:Humans; D016898:Interferon-alpha; D012333:RNA, Messenger; D012859:Sjogren's Syndrome
D016898:Interferon-alpha; D012333:RNA, Messenger