Dual feeding strategy for the production of alpha-amylase by Bacillus caldolyticus using complex media.
In this study, the objective was to investigate an exponential feeding strategy for fed-batch production of thermostable alpha-amylase (E.C. 3.2.1.1.) from the Bacillus caldolyticus (DSM405). The parameters for establishing compositions of feed media and feeding rate were obtained by statistical analysis of batch and continuous shake flask experiments. These parameters were casitone to starch ratio of 2.67g(casitone)g(starch)(-1), maintenance coefficient 0.174g(casitone)g(DW)(-1)h(-1), cell yield 0.62g(DW)g(casitone)(-1) and mu(opt)=0.2h(-1). The exponentially fed fermentation resulted in yield of 120Uml(-1) alpha-amylase that was thermostable up to 105 degrees C. Results of the exponentially fed fermentation have been discussed in the light of a feed-back controlled fed-batch fermentation reported earlier by the authors. A comparison of the temperature and pH effects on amylase produced by B. caldolyticus and on several other commercially available amylases has also been presented.
Schwab
Karima
K
University of Applied Sciences, FBV - Life Sciences and Technology, Seestrasse 64, 13347 Berlin, Germany.
Bader
Johannes
J
Brokamp
Christian
C
Popović
Milan K
MK
Bajpai
Rakesh
R
Berovic
Marin
M
D001407:Bacillus / Q000187:drug effects* / Q000201:enzymology*; D019149:Bioreactors / Q000382:microbiology; D001709:Biotechnology / Q000379:methods*; D002364:Caseins / Q000494:pharmacology; D003470:Culture Media / Q000494:pharmacology*; D004795:Enzyme Stability / Q000187:drug effects; D005285:Fermentation / Q000187:drug effects; D006863:Hydrogen-Ion Concentration / Q000187:drug effects; D012996:Solutions; D013171:Spores, Bacterial / Q000166:cytology / Q000187:drug effects; D013696:Temperature; D000516:alpha-Amylases / Q000096:biosynthesis*
D002364:Caseins; D003470:Culture Media; D012996:Solutions; C475729:casein hydrolysate; D000516:alpha-Amylases