Development of an antigen-based rapid diagnostic test for the identification of blowfly (Calliphoridae) species of forensic significance.
In this study we examine the limitations of currently used sequence-based approaches to blowfly (Calliphoridae) identification and evaluate the utility of an immunological approach to discriminate between blowfly species of forensic importance. By investigating antigenic similarity and dissimilarity between the first instar larval stages of four forensically important blowfly species, we have been able to identify immunoreactive proteins of potential use in the development of species-specific immuno-diagnostic tests. Here we outline our protein-based approach to species determination, and describe how it may be adapted to develop rapid diagnostic assays for the 'on-site' identification of blowfly species.
McDonagh
Laura
L
Hatherly Laboratories, School of Biosciences, University of Exeter, Prince of Wales Road, Exeter, EX4 4PS, UK.
Thornton
Chris
C
Wallman
James F
JF
Stevens
Jamie R
JR
D000818:Animals; D000941:Antigens / Q000097:blood; D015415:Biomarkers / Q000097:blood; D003955:Diagnostic Tests, Routine / Q000379:methods*; D004175:Diptera / Q000145:classification / Q000235:genetics*; D004772:Entomology; D005554:Forensic Medicine / Q000379:methods*; D006801:Humans; D007814:Larva / Q000276:immunology; D020543:Proteome / Q000276:immunology*; D040901:Proteomics / Q000379:methods; D013045:Species Specificity; D013056:Spectrophotometry, Ultraviolet; D013997:Time Factors
D000941:Antigens; D015415:Biomarkers; D020543:Proteome