GATA-1, G208S macrothrombocytes are deficient in talin: immunofluorescence studies.
Previous investigations from our laboratory identified the ultrastructural pathology and cytochemistry of macrothrombocytes (MTC) from patients with the X-linked, G208S varient of the GATA-1 mutation.A subsequent biochemical study of the MTC cytoskeletal proteins using polyacrylamide gel electrophoresis and western blot analysis revealed the MTC were deficient in the high-molecular weight, actin binding protein, talin. The present study has used immunofluorescent techniques to further characterize the talin deficiency. Results confirm that the GATA-1, G208S MTC are deficient in talin, and what little is present relocates to the undersurface of the plasma membrane following activations where it associates with adhesion plaques.
Department of Laboratory Medicine, Pathology and Pediatrics, University of Minnesota School of Medicine, Minneapolis, MN, USA. White003@umn.edu
James G
JG
White
Steven M
SM
Burris
Brian R
BR
Crowe
D001792:Blood Platelets / Q000378:metabolism* / Q000648:ultrastructure; D002462:Cell Membrane / Q000378:metabolism / Q000648:ultrastructure; D050982:GATA1 Transcription Factor / Q000235:genetics / Q000378:metabolism*; D040181:Genetic Diseases, X-Linked / Q000097:blood*; D006801:Humans; D066298:In Vitro Techniques; D009154:Mutation; D016608:Talin / Q000097:blood*
D050982:GATA1 Transcription Factor; C494688:GATA1 protein, human; D016608:Talin