Analysis of genetic diversity of Persea bombycina "Som" using RAPD-based molecular markers.
The utility of RAPD markers in assessing genetic diversity and phenetic relationships in Persea bombycina, a major tree species for golden silk (muga) production, was investigated using 48 genotypes from northeast India. Thirteen RAPD primer combinations generated 93 bands. On average, seven RAPD fragments were amplified per reaction. In a UPGMA phenetic dendrogram based on Jaccard's coefficient, the P. bombycina accessions showed a high level of genetic variation, as indicated by genetic similarity. The grouping in the phenogram was highly consistent, as indicated by high values of cophenetic correlation and high bootstrap values at the key nodes. The accessions were scattered on a plot derived from principal correspondence analysis. The study concluded that the high level of genetic diversity in the P. bombycina accessions may be attributed to the species' outcrossing nature. This study may be useful in identifying diverse genetic stocks of P. bombycina, which may then be conserved on a priority basis.
Bhau
Brijmohan Singh
BS
Plant Genomics Laboratory, Medicinal Aromatic and Economic Plants Division, North-East Institute of Science and Technology, Jorhat, Assam, India. bsbhau@gmail.com
Medhi
Kalyani
K
Das
Ambrish P
AP
Saikia
Siddhartha P
SP
Neog
Kartik
K
Choudhury
S N
SN
D018744:DNA, Plant / Q000235:genetics; D005819:Genetic Markers / Q000235:genetics; D014644:Genetic Variation; D005838:Genotype; D027421:Persea / Q000145:classification / Q000235:genetics*; D010802:Phylogeny; D011110:Polymorphism, Genetic; D025341:Principal Component Analysis; D019105:Random Amplified Polymorphic DNA Technique
D018744:DNA, Plant; D005819:Genetic Markers