Corosolic acid stimulates osteoblast differentiation by activating transcription factors and MAP kinases.
Recently, the use of anabolic agents to enhance bone mass has been a source of interest. Previous work by us suggested that corosolic acid (2alpha-hydroxyursolic acid), an active component of banaba leaves (Lagerstroemia speciosa L.), potentially stimulates the differentiation of mouse osteoblasts. Therefore, the present study investigated whether corosolic acid stimulates osteoblast differentiation, and its possible mechanisms of action. At low concentrations (up to 5 microm), corosolic acid significantly stimulated osteoblast differentiation and mineralization without cytotoxicity. Corosolic acid induced NF-kappaB and MAP kinase activity at an early stage of osteoblast differentiation and increased the activity of the transcription factor AP-1 during late-stage osteoblast differentiation. These results suggest that the anabolic effects of corosolic acid upon osteoblast differentiation could result from its activation of transcription factors and MAP kinases.
Laboratory of Chemical Genomics, Korea Research Institute of Chemical Technology, Daejeon, Korea.
Ki Shuk
KS
Shim
Su-Ui
SU
Lee
Shi Yong
SY
Ryu
Yong Ki
YK
Min
Seong Hwan
SH
Kim
D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects*; D002460:Cell Line; D002470:Cell Survival; D005786:Gene Expression Regulation; D031563:Lagerstroemia / Q000737:chemistry; D051379:Mice; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism*; D016328:NF-kappa B / Q000378:metabolism; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects*; D014157:Transcription Factors / Q000378:metabolism*; D014315:Triterpenes / Q000494:pharmacology*
D016328:NF-kappa B; D014157:Transcription Factors; D014315:Triterpenes; C113861:corosolic acid; D020928:Mitogen-Activated Protein Kinases