Relationship between mast cells and the colitis with relapse induced by trinitrobenzesulphonic acid in Wistar rats.
The present study aimed to clarify the role of mast cells in colitis with relapse induced in Wistar rats by trinitrobenzenosulphonic acid. Colitis induction increased the histamine concentration in the colon, which peaked on day 26. The number of mast cells, probably immature, was ten times higher on day 8. Different from animals infected with intestinal parasites, after colitis remission, mast cells do not migrate to the spleen, showing that mast cell proliferation presents different characteristics depending on the inflammation stimuli. Treatment with sulfasalazine, doxantrazole, quercetin, or nedocromil did not increase the histamine concentration or the mast cell number in the colon on day 26, thereby showing absence of degranulation of these cells. In conclusion, although mast cell proliferation is associated with colitis, these cells and their mediators appear to play no clear role in the colitis with relapses.
Department of Pharmacology, Institute of Biosciences, São Paulo State University (UNESP), 18618-000 Botucatu, SP, Brazil. caroluchini@hotmail.com
Ana Carolina
AC
Luchini
Déborah Mara Costa
DM
de Oliveira
Cláudia Helena
CH
Pellizzon
Luiz Claudio
LC
Di Stasi
José Carlos
JC
Gomes
D000818:Animals; D003092:Colitis / Q000139:chemically induced* / Q000378:metabolism / Q000473:pathology*; D003106:Colon / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D005765:Gastrointestinal Agents / Q000494:pharmacology; D006632:Histamine / Q000378:metabolism; D008297:Male; D008407:Mast Cells / Q000187:drug effects / Q000378:metabolism / Q000473:pathology*; D008853:Microscopy; D017835:Nedocromil / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D012460:Sulfasalazine / Q000494:pharmacology; D013892:Thioxanthenes / Q000494:pharmacology; D014302:Trinitrobenzenesulfonic Acid / Q000494:pharmacology*; D044004:Xanthones / Q000494:pharmacology
D005765:Gastrointestinal Agents; D013892:Thioxanthenes; D044004:Xanthones; D017835:Nedocromil; D012460:Sulfasalazine; D006632:Histamine; D014302:Trinitrobenzenesulfonic Acid; C010134:doxantrazole