Poly(D,L-lactide-co-glycolide) nanoparticles encapsulated fluorescent isothiocyanate and paclitaxol: preparation, release kinetics and anticancer effect.
Poly(D,L-lactide-co-glycolide) (PLGA) is a biodegradable and biocompatible polymer material for drug deliver system. The aim of this study is to synthesize drug-loaded PLGA nanoparticles for sustained release and its anticancer effect in vitro. PLGA nanoparticles were prepared with modified solvent evaporation method. PLGA nanoparticles encapsulated fluorescent isothiocyanate (FITC, as a model drug) and paclitaxol (therapeutic drug) were prepared with the diameter of within 800 nm as drug carrier. The release kinetics and anticancer effect for HeLa cells of the PLGA nanoparticles were further investigated. There was a peak of accumulative FITC release from the FITC-loaded PLGA nanoparticles at approximate 18 h. The inhibition rate of HeLa cell growth was studied by 3-(4,5-dimethylthiazol-2-yl)-3,5-diphenyltetrazolium (MTT) colorimetric assay. Cells were killed by paclitaxol-loaded PLGA nanoparticles. Apoptosis of HeLa cells maybe also occurred due to the sustained release of paclitaxol from the PLGA nanoparticles, which showed that PLGA nanoparticles encapsulated paclitaxol are promising as a controlled drug delivery system in future clinic application.
Department of Biophysics, School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu, Sichuan 610054, PR China.
Hong
H
Yang
Kai
K
Li
Yiyao
Y
Liu
Zhonghua
Z
Liu
Hirokazu
H
Miyoshi
D000970:Antineoplastic Agents / Q000008:administration & dosage / Q000737:chemistry; D002470:Cell Survival / Q000187:drug effects*; D003692:Delayed-Action Preparations / Q000008:administration & dosage* / Q000737:chemistry*; D004058:Diffusion; D004339:Drug Compounding / Q000379:methods; D006367:HeLa Cells; D006801:Humans; D017879:Isothiocyanates / Q000008:administration & dosage / Q000737:chemistry; D019344:Lactic Acid / Q000737:chemistry*; D008422:Materials Testing; D053758:Nanoparticles / Q000008:administration & dosage* / Q000737:chemistry*; D017239:Paclitaxel / Q000008:administration & dosage* / Q000737:chemistry; D011100:Polyglycolic Acid / Q000737:chemistry*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D013050:Spectrometry, Fluorescence / Q000379:methods
D000970:Antineoplastic Agents; D003692:Delayed-Action Preparations; D017879:Isothiocyanates; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; C037152:isothiocyanic acid; D019344:Lactic Acid; D017239:Paclitaxel