Convulsant effect of diphenyl diselenide in rats and mice and its relationship to plasma levels.
Diphenyl diselenide [(PhSe)2], an organoselenium compound, presents pharmacological and toxicological properties in rodents. The aim of this study was to carry out the determination and quantification of (PhSe)2 in plasma after oral administration (p.o.) of this compound (500 mg/kg), dissolved in canola oil, in rats and mice. The second objective was to verify the involvement of different routes of administration ((p.o.), intraperitoneal (i.p.) and subcutaneous (s.c.)) and vehicle solutions (canola oil and dimethyl sulfoxide (DMSO)) in the appearance of seizure episodes and in the plasmatic levels of (PhSe)2 in rats and mice. Analysis of (PhSe)2 in blood samples was performed by gas chromatography/flame ionized detector system (GC/FID). Rat and mouse peak plasma (PhSe)2 levels were 13.13 and 10.11 microg/ml (C(max)), respectively, and occurred at 0.5h (T(max)) post-dosing. The use of different administration routes (p.o., i.p. and s.c.) and vehicle solutions (canola oil or DMSO) in rats and mice indicated that the appearance of seizures and (PhSe)2 plasmatic levels are dependent of administration routes (i.p.>p.o.>s.c.), vehicle solutions (DMSO>canola oil) and animal species (mice>rat).
Departamento de Química, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Santa Maria, CEP 97105-900, RS, Brazil.
Marina
M
Prigol
Ricardo F
RF
Schumacher
Cristina
C
WayneNogueira
Gilson
G
Zeni
D000284:Administration, Oral; D000818:Animals; D001555:Benzene Derivatives / Q000008:administration & dosage / Q000009:adverse effects* / Q000097:blood*; D004121:Dimethyl Sulfoxide; D004305:Dose-Response Relationship, Drug; D005229:Fatty Acids, Monounsaturated; D007274:Injections, Intraperitoneal; D007279:Injections, Subcutaneous; D008297:Male; D051379:Mice; D016566:Organoselenium Compounds / Q000008:administration & dosage / Q000009:adverse effects* / Q000097:blood*; D000074262:Rapeseed Oil; D051381:Rats; D017208:Rats, Wistar; D012640:Seizures / Q000097:blood / Q000139:chemically induced*; D012996:Solutions; D013045:Species Specificity; D013997:Time Factors
D001555:Benzene Derivatives; D005229:Fatty Acids, Monounsaturated; D016566:Organoselenium Compounds; D000074262:Rapeseed Oil; D012996:Solutions; C061132:diphenyldiselenide; D004121:Dimethyl Sulfoxide