Decreased expression of TGF-beta, GILT and T-cell markers in the early stages of soybean enteropathy in Atlantic salmon (Salmo salar L.).
This study investigated the early expression of T-cell markers and genes potentially involved in the induction of soybean meal (SBM) enteropathy in the distal intestine (DI) of Atlantic salmon (Salmo salar L.). Quantitative PCR was used to study the expression of CD3, CD8beta, transforming growth factor beta (TGF-beta), interferon-gamma-inducible lysosomal thiol reductase (GILT) and interleukin-1beta (IL-1beta) in salmon fed SBM for 1, 3 and 7 days using fish fed fishmeal as controls. In the same tissue, the morphological development of SBM enteropathy was evaluated by routine histology and the presence of T cells was mapped by immunohistochemistry. TGF-beta was significantly down-regulated on all days of feeding SBM. GILT was significantly down-regulated on days 3 and 7 compared to day 1. A depression in the expression of T-cell markers was observed on day 3 whereas increased densities of T cells were observed at the base of mucosal folds after 7 days of feeding SBM. Down-regulation of GILT and TGF-beta may lead to sensitization of intraepithelial lymphocytes and failure to maintain normal mucosal integrity in the DI. These responses are implicated in the pathogenesis of SBM enteropathy in Atlantic salmon.
Aquaculture Protein Centre, Norwegian Centre of Excellence, As, Norway.
Einar
E
Lilleeng
Michael H
MH
Penn
Oyvind
O
Haugland
Cheng
C
Xu
Anne Marie
AM
Bakke
Ashild
A
Krogdahl
Thor
T
Landsverk
Marianne K
MK
Frøystad-Saugen
D000821:Animal Feed / Q000009:adverse effects*; D000818:Animals; D017252:CD3 Complex / Q000378:metabolism; D016827:CD8 Antigens / Q000378:metabolism; D003001:Cloning, Molecular; D017931:DNA Primers / Q000235:genetics; D004751:Enteritis / Q000139:chemically induced / Q000276:immunology / Q000473:pathology / Q000662:veterinary*; D005393:Fish Diseases / Q000139:chemically induced* / Q000276:immunology* / Q000473:pathology; D005786:Gene Expression Regulation / Q000276:immunology*; D007150:Immunohistochemistry / Q000662:veterinary; D053583:Interleukin-1beta / Q000378:metabolism; D010957:Plasmids / Q000235:genetics; D020317:Salmo salar; D013025:Glycine max / Q000633:toxicity*; D016212:Transforming Growth Factor beta / Q000235:genetics / Q000378:metabolism
D017252:CD3 Complex; D016827:CD8 Antigens; C477867:CD8beta antigen; D017931:DNA Primers; D053583:Interleukin-1beta; D016212:Transforming Growth Factor beta