Analysis of a consensus fragment in ERIC-PCR fingerprinting of Enterobacter sakazakii.
Enterobacterial Repetitive Intergenic Consensus (ERIC)-PCR was employed to generate ERIC-PCR fingerprints of 23 strains of E. sakazakii. A consensus fragment was observed in all 23 strains and was purified, cloned and sequenced from type strain ATCC29544. A comparison of the nucleotide acid with other sequences available in the GenBank revealed 98% of identity with E. sakazakii ATCC BAA-894 and 71%-75% identity with oligopeptidase gene or protease II gene of some species from the Enterobacteriaceae family. The consensus fragment from ATCC29544 was used to synthesize probes using DIG-labeling by PCR for dot hybridization. The consensus fragment was found to be highly conserved. Diversity analysis based on the consensus fragment sequencing showed a high heterogeneity between E. sakazakii strains and other related strains. In addition, 24 E. sakazakii strains could be distributed into two groups, while E. sakazakii ATCC51329 formed a separate branch. Genetic diversity and potential taxonomic complexity were evident within the E. sakazakii strains.
Ye
Yingwang
Y
South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China.
Wu
Qingping
Q
Yao
Lin
L
Dong
Xiaohui
X
Wu
Kui
K
Zhang
Jumei
J
D044083:Cronobacter sakazakii / Q000145:classification* / Q000235:genetics*; D016172:DNA Fingerprinting; D004269:DNA, Bacterial / Q000032:analysis*; D005506:Food Contamination / Q000032:analysis / Q000517:prevention & control; D014644:Genetic Variation; D015151:Immunoblotting; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012689:Sequence Homology, Nucleic Acid
D004269:DNA, Bacterial