Complement activation by carotenoid derived aldehydes in cultured human vein epithelial cells.
This study was designed to determine the complement activation effects of carotenoid-derived aldehydes (CDA) on cultured human umbilical vein endothelial cells (HUVEC). A dose-dependent complement activation upon incubation of HUVEC with CDA was observed. Interestingly, the data showed that the alternative pathway was not activated. In addition, upon CDA treatment a significant number of apoptotic cells was also observed. The results revealed that CDA could activate the complement by way of the classical pathway. The study suggests that high carotenoid supplementation for the treatment of coronary heart disease should be used cautiously.
Department of Physiology, School of Medicine, Hangzhou Normal University, Hangzhou 310036, China.
Pei-Lin
PL
Wu
Guo-Hua
GH
Lin
Xiao-Dong
XD
Kang
Hong
H
Yuan
Yue-Liang
YL
Shen
D000447:Aldehydes / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D002338:Carotenoids / Q000494:pharmacology*; D002470:Cell Survival; D002478:Cells, Cultured; D003167:Complement Activation / Q000187:drug effects*; D003170:Complement Pathway, Alternative / Q000187:drug effects; D003171:Complement Pathway, Classical / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D042783:Endothelial Cells / Q000378:metabolism*; D006801:Humans; D014471:Umbilical Veins / Q000166:cytology
D000447:Aldehydes; D002338:Carotenoids