Ultraviolet A-induced peroxidation of phosphatidylcholine in unilamellar liposomes.
Unilamellar liposomes of 1-palmitoyl-2-linoleoyl-3-sn-phosphatidylcholine (PLPC) were exposed to ultraviolet A (UVA) light at 37 degrees C. The UVA-induced PLPC peroxidation was inhibited by free radical scavengers, while singlet oxygen quenchers showed slight inhibition. When pre-existing hydroperoxides were reduced, UVA irradiation could not initiate the lipid peroxidation. The UVA-induced reaction of PLPC hydroperoxides with alpha-tocopherol produced addition products. The results indicate that UVA irradiation might dissociate hydroperoxides to alkoxyl and peroxyl radicals which stimulate lipid peroxidation.
The United Graduate School of Agricultural Science, Gifu University, Gifu, Japan.
Azzedine
A
Mazari
Satoshi
S
Iwamoto
Ryo
R
Yamauchi
D002851:Chromatography, High Pressure Liquid; D006861:Hydrogen Peroxide / Q000737:chemistry / Q000494:pharmacology; D015227:Lipid Peroxidation / Q000187:drug effects / Q000528:radiation effects*; D009943:Organophosphorus Compounds / Q000494:pharmacology; D010713:Phosphatidylcholines / Q000378:metabolism*; D014466:Ultraviolet Rays / Q000009:adverse effects*; D053835:Unilamellar Liposomes / Q000378:metabolism*
D009943:Organophosphorus Compounds; D010713:Phosphatidylcholines; D053835:Unilamellar Liposomes; C061896:triphenylphosphine; C055402:1-palmitoyl-2-linoleoylphosphatidylcholine; D006861:Hydrogen Peroxide