High-level biosynthesis of the anteiso-C(17) isoform of the antibiotic mycosubtilin in Bacillus subtilis and characterization of its candidacidal activity.
High-level production (880 mg liter(-1)) and isolation of the anteiso-C(17) isoform of the lipopeptide mycosubtilin produced by a genetically engineered Bacillus subtilis strain are reported. Antifungal activity of this isoform, as determined via culture and fluorometric and cell leakage assays, suggests its potential therapeutic use as an antifungal agent, in particular against Candida spp.
Centre d'Ingénierie des Protéines, Université de Liège, Institut de Chimie, Belgium. pfickers@ulg.ac.be
Patrick
P
Fickers
Jean-Sébastien
JS
Guez
Christian
C
Damblon
Valérie
V
Leclère
Max
M
Béchet
Philippe
P
Jacques
Bernard
B
Joris
D000935:Antifungal Agents / Q000096:biosynthesis*; D001412:Bacillus subtilis / Q000235:genetics / Q000378:metabolism*; D053898:Biosynthetic Pathways / Q000235:genetics; D002175:Candida / Q000187:drug effects*; D005798:Genes, Bacterial; D005818:Genetic Engineering; D008074:Lipoproteins / Q000096:biosynthesis; D008826:Microbial Sensitivity Tests; D020033:Protein Isoforms / Q000378:metabolism
D000935:Antifungal Agents; D008074:Lipoproteins; D020033:Protein Isoforms; C012448:mycosubtiline