[The effect of carbachol on pulmonary vascular permeability and lung water content during oral fluid resuscitation of burn shock induced by a 50% total body surface area full-thickness flame injury in dogs].
OBJECTIVE
To investigate the effects of carbachol (CAR) on pulmonary vascular permeability and pulmonary water content during oral fluid resuscitation of burn shock.


METHODS
Twelve male Beagle dogs with intubation of carotid artery and jugular vein for 24 hours were subjected to a 50% total body surface area (TBSA) full-thickness burn, then they were equally divided into oral resuscitation (OR) and OR plus CAR groups (OR+CAR). Dogs were given either a glucose-electrolyte solution (GES) in OR group or GES containing CAR (20 microg/kg) in OR+CAR group by gavage within 24 hours after burn. Dogs in each group were given intravenous fluid resuscitation after 24 post burn hour (PBH). The delivery rate and volume of GES was in accordance with that of Parkland formula. Respiratory rate (RR), arterial partial pressure of oxygen (PaO(2)), extravascular lung water index (ELWI) and pulmonary vascular permeability index (PVPI) were determined before burn (0 hour), and at 2, 4, 8, 24, 48 and 72 PBH. At 72 PBH or before death, dogs were sacrificed to collect lung tissue for evaluation of myeloperoxidase (MPO), malondialdehyde (MDA), and assessment of the tissue water content by dry to wet weight.


RESULTS
Compared with those before burn, RR, ELWI and PVPI were greatly increased, and PaO(2) obviously decreased in two groups after burn (all P<0.01). At 72 PBH, PaO(2) returned to pre-burn level, while RR, ELWI and PVPI were still higher than pre-burn levels. RR, ELWI and PVPI at 4, 8 and 24 PBH, and PaO(2) at 8, 24, 48 PBH in OR+CAR group were respectively lower or higher than those in OR group (P<0.05 or P<0.01), but those measurements showed no statistical differences between two groups at 72 PBH (all P>0.05). MPO, MDA and lung water contents in OR+CAR group were significantly lower than those in OR group at 72 PBH [(2.64+/-0.38) U/mg vs.(4.12+/-0.46) U/mg, P<0.01; (3.60+/-0.54) micromol/mg vs.(5.14+/-0.62) micromol/mg, P<0.01; (77.40+/-0.56)% vs. (78.30+/-0.54)%, P<0.01].


CONCLUSIONS
The results indicate that CAR inhibits inflammatory response and oxidative damage in lung tissue, and alleviates pulmonary vascular permeability and lung edema during oral fluid resuscitation of burn shock.
Laboratory of Shock and Organ Dysfunction, Burns Institute, First Affiliated Hospital of PLA General Hospital, Beijing 100048, China. hs82080@yahoo.com.cn
Sen
S
Hu
Jin-wei
JW
Chen
Cheng-mei
CM
Bao
Zhi-yong
ZY
Sheng
D000818:Animals; D001830:Body Surface Area; D002056:Burns / Q000150:complications / Q000628:therapy*; D002199:Capillary Permeability / Q000187:drug effects*; D002217:Carbachol / Q000494:pharmacology*; D004195:Disease Models, Animal; D004285:Dogs; D015633:Extravascular Lung Water; D005440:Fluid Therapy; D008168:Lung / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D008297:Male; D012769:Shock / Q000209:etiology / Q000378:metabolism / Q000503:physiopathology / Q000628:therapy*
D002217:Carbachol