Autoantibody to tumor antigen, alpha 2-HS glycoprotein: a novel biomarker of breast cancer screening and diagnosis.
We sought to identify a new serum biomarker for breast cancer screening and diagnosis using stepwise proteomic analysis of sera from breast cancer patients to detect the presence of autoantibodies that react with urinary protein. Two-dimensional immunoblotting was done for screening autoimmunogenic tumor antigens in the urine of breast cancer patients. Reactive spots were identified by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Among urinary proteins separated by two-dimensional electrophoresis, 13 spots showed strong reactivity with pooled sera from breast cancer patients or control sera. By mass spectrometry, we identified alpha 2-HS glycoprotein (AHSG) as a tumor antigen. Peripheral blood was obtained from 81 women diagnosed with breast cancer before surgery and 73 female donors without evidence of any malignancy for the individual analysis. In one-dimensional Western blot analysis, AHSG autoantibody was detected in 64 of 81 breast cancer patients (79.1%) and in 7 of 73 controls (9.6%). The sensitivity of this test in breast cancer patients was 79.0%. Our results suggest that AHSG and anti-AHSG autoantibody may be useful serum biomarkers for breast cancer screening and diagnosis.
Yi
Jae Kyo
JK
Department of Surgery, Seoul National University College of Medicine, 28 Yongon-dong, Chongno-gu, Seoul 110-744, Korea. dynoh@plaza.snu.ac.kr.
Chang
Jong Wook
JW
Han
Wonshik
W
Lee
Jong Won
JW
Ko
Eunyoung
E
Kim
Dong Hyun
DH
Bae
Ji-Yeon
JY
Yu
Jonghan
J
Lee
Cheolju
C
Yu
Myeong-Hee
MH
Noh
Dong-Young
DY
D000328:Adult; D000951:Antigens, Neoplasm / Q000097:blood* / Q000652:urine; D014408:Biomarkers, Tumor / Q000097:blood* / Q000652:urine; D001798:Blood Proteins / Q000378:metabolism* / Q000652:urine; D015153:Blotting, Western; D001943:Breast Neoplasms / Q000097:blood / Q000175:diagnosis* / Q000652:urine; D055088:Early Detection of Cancer; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D008875:Middle Aged; D012372:ROC Curve; D012680:Sensitivity and Specificity; D060749:alpha-2-HS-Glycoprotein
C556354:AHSG protein, human; D000951:Antigens, Neoplasm; D014408:Biomarkers, Tumor; D001798:Blood Proteins; D060749:alpha-2-HS-Glycoprotein