Rapid detection of yeast rRNA genes with primed in situ (PRINS) labeling.
In yeast, rRNA genes can be detected with the FISH technique using rRNA gene probes. This technique yields reliable, reproducible and precise results, but is time-consuming. Here, the primed in situ DNA synthesis (PRINS) procedure has been optimized for rapid detection of yeast rRNA genes. PRINS, which is as sensitive as PCR and allows cytological localization of analyzed sequences, can be adapted for various screening tests requiring fast labeling of rRNA genes.
Department of Genetics, University of Rzeszow, Rzeszow, Poland. mawnuk@gmail.com
Maciej
M
Wnuk
Anna
A
Lewinska
Monika
M
Bugno
Grzegorz
G
Bartosz
Ewa
E
Slota
D005800:Genes, Fungal; D020401:Primed In Situ Labeling / Q000379:methods*; D012337:RNA, Ribosomal, 18S / Q000235:genetics*; D012680:Sensitivity and Specificity; D015003:Yeasts / Q000235:genetics / Q000302:isolation & purification*
D012337:RNA, Ribosomal, 18S