Development of a homologous transformation system for Aspergillus aculeatus based on the sC gene encoding ATP-sulfurylase.
A homologous transformation system was developed using the endogenous ATP-sulfurylase gene, AasC, as a selectable marker in Aspergillus aculeatus. Spontaneous mutation was proved to be beneficial in isolating AasC-deficient mutants. Molecular analysis of sC(+) transformants revealed that the frequency of single copy integration at ATP-sulfurylase locus was more than 40%.
Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Sakai, Osaka, Japan.
Hiromi
H
Adachi
Shuji
S
Tani
Shin
S
Kanamasa
Jun-ichi
J
Sumitani
Takashi
T
Kawaguchi
D001230:Aspergillus / Q000235:genetics*; D005818:Genetic Engineering / Q000379:methods*; D016678:Genome; D009154:Mutation; D013430:Sulfate Adenylyltransferase / Q000235:genetics*; D014170:Transformation, Genetic
D013430:Sulfate Adenylyltransferase