The effect of the shape of single, sub-ms voltage pulses on the rates of surface immobilization and hybridization of DNA.
Electric fields generated by single square and sinusoidal voltage pulses with amplitudes below 2 V were used to assist the covalent immobilization of single-stranded, thiolated DNA probes, onto a chemically functionalized SiO2 surface and to assist the specific hybridization of single-stranded DNA targets with immobilized complementary probes. The single-stranded immobilized DNA probes were either covalently immobilized (chemisorption) or electrostatically adsorbed (physisorption) to a chemically functionalized surface. Comparing the speed of electric field assisted immobilization and hybridization with the corresponding control reactions (without electric field), an increase of several orders of magnitude is observed, with the reaction timescaled down from 1 to 2 h to a range between 100 ns and 1 ms. The influence of the shape of the voltage pulse (square versus sinusoidal) and its duration were studied for both immobilization and hybridization reactions. The results show that pulsed electric fields are a useful tool to achieve temporal and spatial control of surface immobilization and hybridization reactions of DNA.
Cabeça
R
R
INESC Microsistemas e Nanotecnologias and IN-Institute of Nanoscience and Nanotechnology, Rua Alves Redol, 9, 1000-029 Lisbon, Portugal. rcabeca@inesc-mn.pt
Rodrigues
M
M
Prazeres
D M F
DM
Chu
V
V
Conde
J P
JP
D004247:DNA / Q000378:metabolism; D004560:Electricity; D009693:Nucleic Acid Hybridization / Q000379:methods*
D004247:DNA