Smart conferring of nuclease resistance to DNA by 3'-end protection using 2',4'-bridged nucleoside-5'-triphosphates.
Incorporation of 2',4'-bridged nucleotides into the 3'-end of oligodeoxyribonucleotide (ODN) was examined using terminal deoxynucleotidyl transferase (TdT). The three types of 2',4'-bridged nucleoside-5'-triphospates with different bridging structures used were incorporated efficiently into the 3'-end of DNA by TdT, although only single nucleotide incorporation was observed. Nuclease resistance was conferred on DNA, depending on the types of bridging nucleotides added.
Department of Chemistry and Chemical Biology, Graduate School of Engineering, Gunma University, Gunma, Japan. kuwahara@chem-bio.gunma-u.ac.jp
Masayasu
M
Kuwahara
Satoshi
S
Obika
Hidetoshi
H
Takeshima
Yoshihiko
Y
Hagiwara
Jun-Ichi
J
Nagashima
Hiroaki
H
Ozaki
Hiroaki
H
Sawai
Takeshi
T
Imanishi
D004247:DNA / Q000737:chemistry / Q000378:metabolism*; D004253:DNA Nucleotidylexotransferase / Q000378:metabolism; D003851:Deoxyribonucleases / Q000378:metabolism*; D009711:Nucleotides / Q000737:chemistry*; D009838:Oligodeoxyribonucleotides / Q000737:chemistry / Q000378:metabolism*
C541966:KTP compound; C541967:LTP compound; C541968:MTP compound; D009711:Nucleotides; D009838:Oligodeoxyribonucleotides; D004247:DNA; D004253:DNA Nucleotidylexotransferase; D003851:Deoxyribonucleases