[Sesquiterpene lactones of the plants Viguiera sylvatica and Decachaeta thieleana (Asteraceae) modulate nitric oxide production and phagocytosis of macrophages cell line RAW].
Different species of the Asteraceae family are a potential source of sesquiterpene lactones with anti-inflammatory properties. Macrophages play a central role in the regulation of immune responses. In the present study, the in vitro effect of two sesquiterpene lactones, a millerenolide and a thieleanin, was assessed by measuring the production of nitric oxide (NO) by cell line RAW (murine macrophages) using the Griess reagent. Additionally, the effect of these sesquiterpene lactones on phagocytic capacity of latex particles and the reduction of nitroblue tetrazolium (NBT) were evaluated microscopically. Treatment of macrophages with > 2.5 microg/ml of both sesquiterpene lactones, reduced the production of NO. A decreased number of macrophages able to reduce NBT were observed when these cells were treated with 3 microg/ml of millerenolide or 7.5 microg/ml of thieleanin. Treatment of macrophages with 4 microg/ml ofmillerenolide or 7.5 microg/ml of thieleanin, reduced the phagocytic capacity of macrophages. Cytotoxic effects on the macrophages were only observed when the concentration was increased to 8 microg/ml of millerenolide or 25 microg/ml of thieleanin. Our results suggest that these sesquiterpene lactones could be useful compounds in the elaboration of anti-inflammatory drugs.
Centro para Investigaciones en Biología Celular y Molecular (CIBCM), Universidad de Costa Rica, San Pedro, San José, Costa Rica. omarielsag@yahoo.com
Omar A
OA
Dupuy
Renato
R
Murillo
José A
JA
Bonilla
D000818:Animals; D019659:Asteraceae / Q000737:chemistry*; D002460:Cell Line; D007783:Lactones / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D008264:Macrophages / Q000187:drug effects* / Q000502:physiology; D051379:Mice; D009569:Nitric Oxide / Q000096:biosynthesis*; D010587:Phagocytosis / Q000187:drug effects*; D012717:Sesquiterpenes / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*
D007783:Lactones; D012717:Sesquiterpenes; C532684:millerenolide; C533420:thieleanin; D009569:Nitric Oxide