Fullerene derivatives protect endothelial cells against NO-induced damage.
Functional fullerene derivatives have been demonstrated with potent antioxidation properties. Nitric oxide (NO) is a free radical that plays a part in leading to brain damage when it is accumulated to a high concentration. The possible scavenging activity of NO by the hydroxylated fullerene derivative C60(OH)22 and malonic acid derivative C60(C(COOH)2)2 was investigated using primary rat brain cerebral microvessel endothelial cells (CMECs). Results demonstrate that sodium nitroprusside (SNP), used as an NO donor, caused a marked decrease in cell viability and an increase in apoptosis. However, fullerene derivatives can remarkably protect against the apoptosis induced by NO assault. In addition, fullerene derivatives can also prevent NO-induced depolymerization of cytoskeleton and damage of the nucleus and accelerate endothelial cell repair. Further investigation shows that the sudden increase of the intercellular reactive oxygen species (ROS) induced by NO was significantly attenuated by post-treatment with fullerene derivatives. Our results suggest that functional fullerene derivatives are potential applications for NO-related disorders.
CAS Key Laboratory for Biological Effects of Nanomaterials and Nanosafety, National Center for Nanoscience and Technology (NCNST), Beijing 100190, People's Republic of China.
Fang
F
Lao
Wei
W
Li
Dong
D
Han
Ying
Y
Qu
Ying
Y
Liu
Yuliang
Y
Zhao
Chunying
C
Chen
D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D002467:Cell Nucleus / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects*; D002478:Cells, Cultured; D003599:Cytoskeleton / Q000187:drug effects; D042783:Endothelial Cells / Q000502:physiology*; D016166:Free Radical Scavengers / Q000494:pharmacology*; D037741:Fullerenes / Q000494:pharmacology*; D008314:Malonates / Q000494:pharmacology; D046529:Microscopy, Electron, Transmission; D009569:Nitric Oxide / Q000378:metabolism*; D020030:Nitric Oxide Donors / Q000494:pharmacology; D009599:Nitroprusside / Q000494:pharmacology; D051381:Rats; D017382:Reactive Oxygen Species / Q000378:metabolism
D016166:Free Radical Scavengers; D037741:Fullerenes; D008314:Malonates; D020030:Nitric Oxide Donors; D017382:Reactive Oxygen Species; C496652:fullerol C60(OH)22; C107223:dimalonic acid C(60); D009599:Nitroprusside; D009569:Nitric Oxide