CD30 expression reveals that culture adaptation of human embryonic stem cells can occur through differing routes.
Human embryonic stem cells undergo adaptive changes that can increase their growth capacity upon prolonged culture in vitro. This is frequently associated with nonrandom karyotypic changes, commonly involving amplification of genetic material from chromosomes 12, 17, and X. A recent study suggested that the karyotypically abnormal cells can be identified by their expression of CD30, which confers resistance to apoptosis. We have now investigated CD30 expression and apoptosis in karyotypically normal and abnormal sublines of the human ES cell line, H7, but our results were contrary to those previously observed. In this cell line, CD30 expression did not segregate the normal and abnormal cells, and abnormal cells were not protected from apoptosis. These data suggest that culture adaptation can occur through a variety of mechanisms.
Centre for Stem Cell Biology, University of Sheffield, Western Bank, Sheffield, UK.
Neil J
NJ
Harrison
James
J
Barnes
Mark
M
Jones
Duncan
D
Baker
Paul J
PJ
Gokhale
Peter W
PW
Andrews
D000220:Adaptation, Biological; D017209:Apoptosis; D018236:Carcinoma, Embryonal / Q000378:metabolism / Q000473:pathology; D002470:Cell Survival; D002478:Cells, Cultured; D053595:Embryonic Stem Cells / Q000378:metabolism*; D005434:Flow Cytometry; D006801:Humans; D007621:Karyotyping; D017730:Ki-1 Antigen / Q000378:metabolism*
D017730:Ki-1 Antigen