Dichromate effect on energy dissipation of photosystem II and photosystem I in Chlamydomonas reinhardtii.
In this study, we investigated the energy dissipation processes via photosystem II and photosystem I activity in green alga Chlamydomonas reinhardtii exposed to dichromate inhibitory effect. Quantum yield of photosystem II and also photosystem I were highly decreased by dichromate effect. Such inhibition by dichromate induced strong quenching effect on rapid OJIP fluorescence transients, indicating deterioration of photosystem II electron transport via plastoquinone pool toward photosystem I. The decrease of energy dissipation dependent on electron transport of photosystem II and photosystem I by dichromate effect was associated with strong increase of non-photochemical energy dissipation processes. By showing strong effect of dichromate on acceptor side of photosystem I, we indicated that dichromate inhibitory effect was not associated only with PSII electron transport. Here, we found that energy dissipation via photosystem I was limited by its electron acceptor side. By the analysis of P700 oxido-reduction state with methylviolagen as an exogenous PSI electron transport mediator, we showed that PSI electron transport discrepancy induced by dichromate effect was also caused by inhibitory effect located beyond photosystem I. Therefore, these results demonstrated that dichromate has different sites of inhibition which are associated with photosystem II, photosystem I and electron transport sink beyond photosystems.
Perreault
François
F
Department of Chemistry, University of Quebec in Montreal, C.P. 8888, Succ. Centre-Ville, Montreal, Quebec, Canada.
Ait Ali
Nadia
N
Saison
Cyril
C
Popovic
Radovan
R
Juneau
Philippe
P
D000818:Animals; D016825:Chlamydomonas reinhardtii / Q000254:growth & development / Q000378:metabolism*; D002734:Chlorophyll / Q000737:chemistry / Q000378:metabolism; D004579:Electron Transport; D004734:Energy Metabolism; D005456:Fluorescent Dyes / Q000737:chemistry / Q000378:metabolism; D045331:Photosystem I Protein Complex / Q000378:metabolism*; D045332:Photosystem II Protein Complex / Q000378:metabolism*; D011192:Potassium Dichromate / Q000494:pharmacology*; D014874:Water Pollutants, Chemical / Q000494:pharmacology*
D005456:Fluorescent Dyes; D045331:Photosystem I Protein Complex; D045332:Photosystem II Protein Complex; D014874:Water Pollutants, Chemical; D002734:Chlorophyll; C001785:chlorophyll P 700; D011192:Potassium Dichromate