Proteomic identification of a monoclonal antibody recognizing caveolin-1 in hepatocellular carcinoma with metastatic potential.
A monoclonal antibody, McAb9E (IgG3), was generated against a metastatic HCC cell line, MHCC-1. The antigen was characterized as human Caveolin-1 (Cav-1, 21kDa), with pI of 5.65. The Cav-1 antigen was found significantly over expressed in metastatic HCC cell lines as well as in tumor specimens. The Cav-1 specific McAb may be a useful molecular agent for metastatic HCC.
Hu
Mei-yu
MY
Experiment Research Center, Zhongshan Hospital, Fudan University, Shanghai, China. liu.kangda@zs-hospital.sh.cn
Lam
Chi-tat
CT
Liu
Kang-da
KD
Xu
Zhi
Z
Fatima
Sarwat
S
Su
Yvonne C F
YC
Tsang
Felice
F
Chen
Jie
J
Pang
Jin-zhong
JZ
Qin
Lun-xiu
LX
Luk
J M
JM
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis* / Q000737:chemistry / Q000276:immunology*; D000918:Antibody Specificity; D006528:Carcinoma, Hepatocellular / Q000276:immunology / Q000473:pathology*; D051242:Caveolin 1 / Q000276:immunology*; D045744:Cell Line, Tumor; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008969:Molecular Sequence Data; D009362:Neoplasm Metastasis; D040901:Proteomics; D053719:Tandem Mass Spectrometry
D000911:Antibodies, Monoclonal; D051242:Caveolin 1