Protein separation and identification using magnetic beads encoded with surface-enhanced Raman spectroscopy.
This article presents a prototype of a surface-enhanced Raman spectroscopy (SERS)-encoded magnetic bead of 8mum diameter. The core part of the bead is composed of a magnetic nanoparticle (NP)-embedded sulfonated polystyrene bead. The outer part of the bead is embedded with Ag NPs on which labeling molecules generating specific SERS bands are adsorbed. A silica shell is fabricated for further bioconjugation and protection of SERS signaling. Benzenethiol, 4-mercaptotoluene, 2-naphthalenethiol, and 4-aminothiophenol are used as labeling molecules. The magnetic SERS beads are used as substrates for protein sensing and screening with easy handling. As a model application, streptavidin-bound magnetic SERS beads are used to illustrate selective separation in a flow cytometry system, and the screened beads are spectrally recognized by Raman spectroscopy. The proposed magnetic SERS beads are likely to be used as a versatile solid support for protein sensing and screening in multiple assay technology.
Jun
Bong-Hyun
BH
School of Chemical and Biological Engineering, Seoul National University, Seoul 151-747, Republic of Korea.
Noh
Mi Suk
MS
Kim
Gunsung
G
Kang
Homan
H
Kim
Jong-Ho
JH
Chung
Woo-Jae
WJ
Kim
Min-Soo
MS
Kim
Yong-Kweon
YK
Cho
Myung-Haing
MH
Jeong
Dae Hong
DH
Lee
Yoon-Sik
YS
D000814:Aniline Compounds / Q000737:chemistry; D001710:Biotin / Q000737:chemistry; D008280:Magnetics; D053768:Metal Nanoparticles / Q000737:chemistry; D009281:Naphthalenes / Q000737:chemistry; D010636:Phenols / Q000737:chemistry; D011137:Polystyrenes / Q000737:chemistry; D011506:Proteins / Q000032:analysis* / Q000302:isolation & purification; D012834:Silver / Q000737:chemistry; D013059:Spectrum Analysis, Raman / Q000379:methods*; D019809:Streptavidin / Q000737:chemistry / Q000302:isolation & purification; D013438:Sulfhydryl Compounds / Q000737:chemistry; D013499:Surface Properties
D000814:Aniline Compounds; D009281:Naphthalenes; D010636:Phenols; D011137:Polystyrenes; D011506:Proteins; D013438:Sulfhydryl Compounds; D012834:Silver; D001710:Biotin; C042983:thiophenol; C064316:4-aminothiophenol; D019809:Streptavidin; C401958:2-naphthalenethiol