Synthesis and base-pairing properties of C-nucleotides having 1-substituted 1H-1,2,3-triazoles.
Oligonucleotides including C-nucleotides having 1-substitued 1H-1,2,3-triazoles as artificial nucleobases were conveniently synthesized by the post-elongation modification method using the copper(I)-catalyzed alkyne-azide 1,3-dipolar cycloaddition (CuAAC) reaction. The base-pairing properties of the triazole nucleobase analogs in forming duplexes with oligonucleotides were investigated by the T(m) experiments.
Nakahara
Motoi
M
Graduate School of Pharmaceutical Sciences, Osaka University, 1-6 Yamadaoka, Suita, Osaka 565-0871, Japan.
Kuboyama
Takeshi
T
Izawa
Akihiro
A
Hari
Yoshiyuki
Y
Imanishi
Takeshi
T
Obika
Satoshi
S
D020029:Base Pairing; D008722:Methods; D009691:Nucleic Acid Denaturation; D009693:Nucleic Acid Hybridization; D009711:Nucleotides / Q000138:chemical synthesis* / Q000737:chemistry; D009841:Oligonucleotides; D044366:Transition Temperature; D014230:Triazoles / Q000737:chemistry*
D009711:Nucleotides; D009841:Oligonucleotides; D014230:Triazoles