Lipoplexes targeting the CD44 hyaluronic acid receptor for efficient transfection of breast cancer cells.
Lipoplexes containing a hyaluronic acid-dioleoylphosphatidylethanolamine (HA-DOPE) conjugate were designed to target the CD44 receptor on breast cancer cells. Cationic liposomes composed of a mixture of [2-(2,3-didodecyloxypropyl)hydroxyethyl]ammonium bromide (DE) and dioleoylphosphatidylethanolamine (DOPE) with or without HA-DOPE were prepared, characterized, and used to form a complex with plasmid DNA pCMV-luc. Lipoplexes displayed a negative zeta potential and a mean diameter between 250-300 nm. Cytotoxicity and transfection efficiency of the lipoplexes were determined on the MDA-MB-231and MCF-7 breast cancer cell lines. Cytotoxicity was not modified by the presence of HA-DOPE. However HA-DOPE increased the level of transfection on CD44-expressing MDA-MB-231 cells compared to the MCF-7 line, which expresses very low levels of CD44. The transfection on the MDA-MB-231 cells was highly inhibited by anti-CD44 Hermes-1 antibody but not by the nonspecific anti-ErbB2 antibody. In conclusion, cationic liposomes containing the HA-DOPE conjugate mediated good transfection on CD44 expressing cell lines in culture.
UMR CNRS 8612, Universite Paris Sud 11, Chatenay-Malabry, F-92290, France.
Claudio
C
Surace
Silvia
S
Arpicco
Amélie
A
Dufaÿ-Wojcicki
Véronique
V
Marsaud
Céline
C
Bouclier
Denis
D
Clay
Luigi
L
Cattel
Jack-Michel
JM
Renoir
Elias
E
Fattal
D001943:Breast Neoplasms / Q000628:therapy*; D002470:Cell Survival; D016503:Drug Delivery Systems; D005260:Female; D018014:Gene Transfer Techniques; D006801:Humans; D018960:Hyaluronan Receptors / Q000276:immunology / Q000378:metabolism*; D006820:Hyaluronic Acid / Q000737:chemistry / Q000378:metabolism*; D008081:Liposomes; D008156:Luciferases / Q000378:metabolism; D010714:Phosphatidylethanolamines / Q000737:chemistry / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014162:Transfection; D014407:Tumor Cells, Cultured
D018960:Hyaluronan Receptors; D008081:Liposomes; D010714:Phosphatidylethanolamines; D012333:RNA, Messenger; C094877:dioleoyl phosphatidylethanolamine; D006820:Hyaluronic Acid; D008156:Luciferases