Gene expression profiling of ErbB receptors and ligands in human meningiomas.
ErbB family receptors mediate major cellular functions implied in tumorigenesis, though their role in meningiomas was not thoroughly studied. Meningiomas represent 30% of primary cranial tumors, are mostly benign, and prevail in the second half of life. Tumor therapy requires information about molecular alterations, thus we studied expression of ErbB receptor and ligand genes by real-time RT-PCR in different meningioma grades. Receptors were overexpressed (ErbB1, ErbB2) or underexpressed (ErbB3, ErbB4). Ligands EGF, TGFA, AREG, DTR, BTD were underexpressed and the neuregulins were overexpressed or underexpressed. A strong ErbB1-ErbB2 correlation was found. These data might be useful for gene therapy.
UMR745, INSERM, Faculté des Sciences Pharmaceutiques et Biologiques, Université Paris-Descartes, Paris, France.
Ingrid
I
Laurendeau
Marcela
M
Ferrer
Delia
D
Garrido
Nicky
N
D'Haene
Patricia
P
Ciavarelli
Armando
A
Basso
Michel
M
Vidaud
Ivan
I
Bieche
Isabelle
I
Salmon
Irene
I
Szijan
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D066258:Amphiregulin; D066259:Betacellulin; D066255:EGF Family of Proteins; D004815:Epidermal Growth Factor / Q000235:genetics; D066261:Epigen; D066246:ErbB Receptors / Q000235:genetics; D005260:Female; D020869:Gene Expression Profiling / Q000379:methods; D015972:Gene Expression Regulation, Neoplastic; D018815:Genes, erbB; D006023:Glycoproteins / Q000235:genetics; D066257:Heparin-binding EGF-like Growth Factor; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins / Q000235:genetics*; D008024:Ligands; D008297:Male; D008577:Meningeal Neoplasms / Q000235:genetics* / Q000473:pathology; D008579:Meningioma / Q000235:genetics* / Q000473:pathology; D008875:Middle Aged; D009367:Neoplasm Staging; D020880:Neuregulins / Q000235:genetics; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000378:metabolism; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000235:genetics; D066247:Receptor, ErbB-4; D016211:Transforming Growth Factor alpha / Q000235:genetics
D066258:Amphiregulin; D066259:Betacellulin; D066255:EGF Family of Proteins; D004815:Epidermal Growth Factor; D066261:Epigen; D066246:ErbB Receptors; D006023:Glycoproteins; D066257:Heparin-binding EGF-like Growth Factor; D036341:Intercellular Signaling Peptides and Proteins; D008024:Ligands; D020880:Neuregulins; D012333:RNA, Messenger; D018719:Erb-b2 Receptor Tyrosine Kinases; D066247:Receptor, ErbB-4; D016211:Transforming Growth Factor alpha; C587848:AREG protein, human; C587851:BTC protein, human; C587854:EPGN protein, human; C587883:HBEGF protein, human; C512478:EGFR protein, human; C508053:ERBB2 protein, human; C587689:ERBB4 protein, human