Blockers of K+/Cl- transporter/channels diminish proliferation of osteoblastic cells.
In the preset report, we studied if blockers of the K+/Cl- cotransporter, and Cl- and K+ channels modify the proliferation of MC3T3-E1 osteoblastic cells. A blocker of the K+/Cl- cotransporter, DIOA (100 and 500 microM), diminished the cell proliferation. NPPB (100 and 300 microM, a blocker of Cl- channel) and quinine (100 and 500 microM, a blocker of K+ channel) also suppressed the proliferation of MC3T3-E1 cells. These blockers of the K+/Cl- cotransporter/channels (DIOA, NPPB and quinine) would increase the intracellular Cl- concentration ([Cl-]i). On the other hand, our previous study (Biochem. Biophys. Res. Commun. 361, 1038-1043, 2007) reported that a decrease in [Cl-]i diminished the proliferation of MC3T3-E1 osteoblastic cells. Taken together, the observations indicate that both increases and decreases in [Cl-]i diminish the proliferation of MC3T3-E1 osteoblastic cells.
Department of Orthopaedics, Graduate School of Medical Science, Kyoto Prefectural University of Medicine.
Masahiro
M
Maki
Hiroaki
H
Miyazaki
Naomi
N
Niisato
Toru
T
Morihara
Yoshinori
Y
Marunaka
Toshikazu
T
Kubo
D000818:Animals; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D051379:Mice; D010006:Osteoblasts / Q000166:cytology / Q000378:metabolism*; D049994:Sodium Potassium Chloride Symporter Inhibitors / Q000494:pharmacology*; D028021:Sodium-Potassium-Chloride Symporters / Q000378:metabolism*
D049994:Sodium Potassium Chloride Symporter Inhibitors; D028021:Sodium-Potassium-Chloride Symporters