Rapid detection of lactobacillus and yeast concentrations using a particle size distribution analyser.
OBJECTIVE
To see the possibility of particle size distribution analyser (PSDA) in detecting concentration of lactobacillus contaminants in yeast fermentation.


RESULTS
A PSDA was used to rapidly determine the size and concentration of lactobacillus and Saccharomyces cerevisiae. Data showed that the aerodynamic diameters of Lactobacillus casei and S. cerevisiae cells were around 0.63 and 2.9 microm, respectively, with both cultures showing a linear relationship between cell density and particle count on a size distribution curve of PSDA. In addition, Lactobacillus fermentum showed high similarity in bacterial size distribution and particle count numbers with L. casei. The PSDA also rapidly detected (within 1 min) the cell concentrations of S. cerevisiae and L. casei in a mixed sample with different concentration ratios with 10(7)-10(9) cells ml(-1) of detection range.


CONCLUSIONS
PSDA was demonstrated to be useful for the rapid detection of lactobacillus and S. cerevisiae concentrations.


CONCLUSIONS
This is the first report concerning PSDA to detect the concentration of bacteria and yeast. This method can be useful in the actual field during ethanol fermentation because of relatively easy handling and rapid detection.
Department of Environmental Science and Engineering, Gwangju Institute of Science and Technology, Gwangju, Korea.
H
H
Kim
K
K
Park
S
S
Oh
I S
IS
Chang
D015169:Colony Count, Microbial; D007778:Lactobacillus / Q000254:growth & development*; D016533:Mycological Typing Techniques; D010316:Particle Size; D012441:Saccharomyces cerevisiae / Q000254:growth & development*; D013053:Spectrophotometry / Q000379:methods*