Surface-plasmon-enhanced fluorescence from periodic quantum dot arrays through distance control using biomolecular linkers.
We have developed a protein-enabled strategy to fabricate quantum dot (QD) nanoarrays where up to a 15-fold increase in surface-plasmon-enhanced fluorescence has been achieved. This approach permits a comprehensive control both laterally (via lithographically defined gold nanoarrays) and vertically (via the QD-metal distance) of the collectively behaving assemblies of QDs and gold nanoarrays by way of biomolecular recognition. Specifically, we demonstrated the spectral tuning of plasmon resonant metal nanoarrays and self-assembly of protein-functionalized QDs in a stepwise fashion with a concomitant incremental increase in separation from the metal surface through biotin-streptavidin spacer units.
Department of Materials Science and Engineering, University of Washington, Seattle, WA 98195, USA.
Melvin T
MT
Zin
Kirsty
K
Leong
Ngo-Yin
NY
Wong
Hong
H
Ma
Mehmet
M
Sarikaya
Alex K-Y
AK
Jen
D005453:Fluorescence; D011485:Protein Binding; D011506:Proteins / Q000378:metabolism*; D045663:Quantum Dots; D020349:Surface Plasmon Resonance / Q000379:methods*
D011506:Proteins