Identification of natural MHC class II presented phosphopeptides and tumor-derived MHC class I phospholigands.
MHC molecules present protein-derived peptides to T lymphocytes. By developing TiO2-based microcentrifugation columns, we identified the first phosphorylated MHC class I ligands from tumor tissue (renal cell carcinoma) and, by comparison to healthy renal tissue, found one Brf1-derived ligand as potentially tumor-associated. We further discovered the first natural phosphorylated MHC class II ligands. They revealed several novel phosphorylation sites of significant transmembrane receptors, such as Frizzled 6, CXCR4 and CD20.
Meyer
Verena S
VS
Department of Immunology, Institute for Cell Biology, University of Tübingen, D-72076 Tübingen, Germany.
Drews
Oliver
O
Günder
Marc
M
Hennenlotter
Jörg
J
Rammensee
Hans-Georg
HG
Stevanovic
Stefan
S
D018951:Antigens, CD20 / Q000096:biosynthesis; D002292:Carcinoma, Renal Cell / Q000378:metabolism; D045744:Cell Line, Tumor; D051157:Frizzled Receptors / Q000096:biosynthesis; D005805:Genes, MHC Class I; D005802:Genes, MHC Class II; D015395:Histocompatibility Antigens Class I / Q000737:chemistry*; D000949:Histocompatibility Antigens Class II / Q000737:chemistry*; D006801:Humans; D008024:Ligands; D008113:Liver Neoplasms / Q000378:metabolism; D010455:Peptides / Q000737:chemistry*; D010748:Phosphopeptides / Q000737:chemistry*; D010766:Phosphorylation; D019718:Receptors, CXCR4 / Q000096:biosynthesis; D043562:Receptors, G-Protein-Coupled / Q000096:biosynthesis; D014025:Titanium / Q000737:chemistry
D018951:Antigens, CD20; C484687:FZD6 protein, human; D051157:Frizzled Receptors; D015395:Histocompatibility Antigens Class I; D000949:Histocompatibility Antigens Class II; D008024:Ligands; D010455:Peptides; D010748:Phosphopeptides; D019718:Receptors, CXCR4; D043562:Receptors, G-Protein-Coupled; C009495:titanium dioxide; D014025:Titanium