A comparison of cytokine production in 2-dimensional and 3-dimensional cultures of bone marrow stromal cells of multiple myeloma patients in response to RPMI8226 myeloma cells.
We examined cytokine production by bone marrow stromal cells (BMSCs) of patients with multiple myeloma (MM) in response to contact with myeloma RPMI8226 cells in standard 2-dimensional (2D) cultures and in 3-dimensional (3D) cultures on a gelatine sponge scaffold. It was detected that BMSCs in the 3D cultures produced more IL-11 and HGF and less IL-10 than in the 2D cultures. Moreover, RPMI8226 cells after contact with BMSCs in 3D cultures produced more sIL-6R than in the classic 2D cultures. We concluded that 3D cultures of BMSCs with myeloma cells offered a promising model for in vitro examination of interactions between myeloma cells and the bone marrow stroma and for examination of potent antimyeloma agents.
Department of Virology and Immunology, Maria Curie-Skłodowska University, Lublin, Poland. basiaz@biotop.umcs.lublin.pl
Barbara
B
Zdzisińska
Jacek
J
Roliński
Tomasz
T
Piersiak
Martyna
M
Kandefer-Szerszeń
D000368:Aged; D000369:Aged, 80 and over; D001854:Bone Marrow Cells / Q000378:metabolism* / Q000473:pathology*; D018929:Cell Culture Techniques / Q000379:methods*; D016207:Cytokines / Q000096:biosynthesis*; D005260:Female; D006801:Humans; D008297:Male; D018613:Microscopy, Confocal; D008875:Middle Aged; D009101:Multiple Myeloma / Q000378:metabolism* / Q000473:pathology*; D017154:Stromal Cells / Q000378:metabolism* / Q000473:pathology*; D013997:Time Factors; D014407:Tumor Cells, Cultured
D016207:Cytokines