Plasma IL-6, its soluble receptors and F2-isoprostanes at rest and during exercise in chronic fatigue syndrome.
The aim of the current study was to investigate the levels of interleukin-6 (IL-6), its soluble receptors (sIL-6R and sgp130) and F(2)-isoprostanes, at rest and during exercise, in patients with chronic fatigue syndrome (CFS). Six male CFS patients and six healthy controls performed an incremental exercise test to exhaustion and a submaximal exercise bout to exhaustion. Blood samples taken in the submaximal test at rest, immediately post-exercise and 24 h post-exercise were analyzed for IL-6, sIL-6R, sgp130 and F(2)-isoprostanes. A further 33 CFS and 33 healthy control participants gave a resting blood sample for IL-6 and sIL-6R measurement. During the incremental exercise test only power output at the lactate threshold was lower (P<0.05) in the CFS group. F(2)-isoprostanes were higher (P<0.05) in CFS patients at rest and this difference persisted immediately and 24 h post-exercise. The exercise study found no differences in IL-6, sIL-6R or sgp130 at any time point between groups. In the larger resting group, there were no differences in IL-6 and sIL-6R between CFS and control groups. This investigation has demonstrated that patients with CFS do not have altered plasma levels of IL-6, sIL-6R or sgp130 either at rest or following exercise. F(2)-isoprostanes, however, were consistently higher in CFS patients.
Strathclyde Institute of Pharmacy and Biomedical Sciences, University of Strathclyde, Glasgow, UK.
M
M
Robinson
S R
SR
Gray
M S
MS
Watson
G
G
Kennedy
A
A
Hill
J J F
JJ
Belch
M A
MA
Nimmo
D000328:Adult; D000368:Aged; D016022:Case-Control Studies; D050822:Cytokine Receptor gp130 / Q000097:blood* / Q000378:metabolism; D005080:Exercise Test; D028441:F2-Isoprostanes / Q000097:blood* / Q000378:metabolism; D015673:Fatigue Syndrome, Chronic / Q000097:blood* / Q000378:metabolism; D005260:Female; D006801:Humans; D015850:Interleukin-6 / Q000097:blood* / Q000378:metabolism; D007773:Lactates / Q000097:blood; D008297:Male; D008875:Middle Aged; D010101:Oxygen Consumption; D005082:Physical Exertion / Q000502:physiology*; D019947:Receptors, Interleukin-6 / Q000097:blood* / Q000378:metabolism
D028441:F2-Isoprostanes; C508600:IL6 protein, human; C508603:IL6R protein, human; C494199:IL6ST protein, human; D015850:Interleukin-6; D007773:Lactates; D019947:Receptors, Interleukin-6; D050822:Cytokine Receptor gp130