Magnetic nanoparticle of Fe3O4 and 5-bromotetrandrin interact synergistically to induce apoptosis by daunorubicin in leukemia cells.
Apoptosis is a common pathway that finally mediated the killing functions of anticancer drugs, which is an important cause of multidrug resistance (MDR). The aim of this study was to investigate the potential benefit of combination therapy with magnetic nanoparticle of Fe(3)O(4) (MNP(Fe(3)O(4))) and 5-bromotetrandrin (BrTet). Analysis of the apoptosis percentage showed that combination of daunorubicin (DNR) with either MNP(Fe(3)O(4)) or BrTet exerted a potent cytotoxic effect on K562/A02 cells, while MNP(Fe(3)O(4)) and BrTet cotreatment can synergistically enhance DNR-induced apoptosis. Importantly, we confirmed that the distinct synergism effect of that composite on reverse multidrug resistance may owe to the regulation of various proliferative and antiapoptotic gene products, including P53 and caspase-3. Thus our in vitro data strongly suggests a potential clinical application of MNP(Fe(3)O(4)) and BrTet combination on CML.
Department of Hematology, The Affi liated Zhongda Hospital, Clinical Medical School, Southeast University, Nanjing, 210009 P R China.
Baoan
B
Chen
Jian
J
Cheng
Mingfang
M
Shen
Feng
F
Gao
Wenlin
W
Xu
Huilin
H
Shen
Jiahua
J
Ding
Chong
C
Gao
Qian
Q
Sun
Xinchen
X
Sun
Hongyan
H
Cheng
Guohong
G
Li
Wenji
W
Chen
Ningna
N
Chen
Lijie
L
Liu
Xiaomao
X
Li
Xuemei
X
Wang
D000903:Antibiotics, Antineoplastic / Q000008:administration & dosage / Q000737:chemistry; D017209:Apoptosis / Q000187:drug effects*; D044182:Benzylisoquinolines / Q000737:chemistry* / Q000528:radiation effects; D003630:Daunorubicin / Q000008:administration & dosage* / Q000737:chemistry; D004337:Drug Carriers / Q000737:chemistry* / Q000528:radiation effects; D005290:Ferric Compounds / Q000737:chemistry* / Q000528:radiation effects; D006801:Humans; D020014:K562 Cells; D007938:Leukemia / Q000503:physiopathology*; D053758:Nanoparticles / Q000008:administration & dosage / Q000737:chemistry* / Q000528:radiation effects
D000903:Antibiotics, Antineoplastic; D044182:Benzylisoquinolines; D004337:Drug Carriers; D005290:Ferric Compounds; C514835:bromotetrandrine; C000499:ferric oxide; D003630:Daunorubicin