Improvement of hydrogelation abilities and handling of photocurable gelatin-based crosslinking materials.
Three types of eosin-derivatized gelatins (eosin-gelatins) with different molecular weights (M(w)) of ca. 15 kDa (low-molecular-weight eosin-gelatin, LEG), ca. 30 kDa (medium-molecular-weight eosin-gelatin, MEG), and ca. 95 kDa (high-molecular-weight eosin-gelatin, HEG) were prepared. All the eosin-gelatins except for HEG dissolved completely in water at 37 degrees C within several hours even at high concentrations of 35 or 40 wt % along with polyamine (poly(N,N-dimethylaminopropylacrylamide)) to produce photo-crosslinkable materials. The materials had appropriate viscosity for in situ molding at 37 degrees C and could be handled as a liquid at low temperatures of up to 25 degrees C. Upon photoirradiation for several tens of seconds, the materials were converted almost completely to hydrogels in the desired form with a microporous network structure by the radical coupling reaction. The mechanical strength of the produced hydrogels could be controlled by selecting a particular molecular weight or concentration of eosin-gelatins. The hydrogels obtained from LEG (40 wt %) or MEG (35 wt %) had elasticity similar to that of goat periodontal tissue. The handling of the photo-crosslinkable materials at room temperature and their photogelation ability were drastically improved by reducing the M(w) of eosin-gelatin. The potential usefulness of the photo-crosslinkable materials to periodontal regeneration has been discussed.
Department of Dentistry and Oral Surgery, School of Medicine, Keio University, Tokyo, Japan.
Chie
C
Fukaya
Yasuhide
Y
Nakayama
Yoshinobu
Y
Murayama
Sadao
S
Omata
Ayaka
A
Ishikawa
Yasuo
Y
Hosaka
Taneaki
T
Nakagawa
D001672:Biocompatible Materials / Q000737:chemistry; D003432:Cross-Linking Reagents / Q000737:chemistry*; D003764:Dental Materials / Q000737:chemistry; D055119:Elastic Modulus; D004801:Eosine Yellowish-(YS) / Q000737:chemistry; D005780:Gelatin / Q000737:chemistry*; D006801:Humans; D020100:Hydrogels / Q000737:chemistry*; D008422:Materials Testing; D015394:Molecular Structure; D008970:Molecular Weight; D010777:Photochemistry / Q000379:methods*; D011073:Polyamines / Q000737:chemistry; D012038:Regeneration / Q000502:physiology
D001672:Biocompatible Materials; D003432:Cross-Linking Reagents; D003764:Dental Materials; D020100:Hydrogels; D011073:Polyamines; D005780:Gelatin; D004801:Eosine Yellowish-(YS)