Antitumoral effect of IL-12 gene transfected via liposomes into B16F0 cells.
Murine melanoma B16F0 cells were transfected with SA:DPPC:DOPE (2:1:1 molar ratio) liposomes associated with a plasmid encoding murine IL-12. Stearylamine, a cationic lipid, showed a greater transfection efficiency compared to DOTAP-containing liposomes. The lipid:DNA ratio was 2:1 (w/w). Control groups were mock transfected or transfected with an empty plasmid (pNeo). pNeo or IL-12 transfected cells and controls were inoculated intradermically into the dorsal region of the foot or the lateral flank of C57BL6 mice. Results showed that IL-12 expression had a marked effect on in vivo growth of B16 melanoma tumors developed in both anatomic sites, significantly retarding their growth and prolonging host survival.
Laboratorio de Biomembranas, Departamento de Ciencia y Tecnologia, Universidad Nacional de Quilmes, Buenos Aires, Argentina.
Lucía
L
Speroni
Julieta
J
Gasparri
Victoria
V
de los A Bustuoabad
Nadia S
NS
Chiaramoni
Andrzej
A
Smagur
Stanisław
S
Szala
María C
MC
Taira
Silvia
S
del V Alonso
D000588:Amines; D000704:Analysis of Variance; D000818:Animals; D045744:Cell Line, Tumor; D002470:Cell Survival; D018450:Disease Progression; D005229:Fatty Acids, Monounsaturated; D017930:Genes, Reporter; D018664:Interleukin-12 / Q000235:genetics* / Q000378:metabolism; D008081:Liposomes; D008546:Melanoma, Experimental / Q000378:metabolism / Q000401:mortality / Q000473:pathology / Q000628:therapy*; D051379:Mice; D008810:Mice, Inbred C57BL; D009368:Neoplasm Transplantation; D000644:Quaternary Ammonium Compounds; D014162:Transfection / Q000379:methods*; D047368:Tumor Burden
D000588:Amines; D005229:Fatty Acids, Monounsaturated; D008081:Liposomes; D000644:Quaternary Ammonium Compounds; D018664:Interleukin-12; C009317:stearylamine; C070046:1,2-dioleoyloxy-3-(trimethylammonium)propane