A simple method for monitoring protein-DNA interactions.
A simple, efficient and cheap method is reported for monitoring interactions between single stranded desoxyribonucleic acids and proteins, using fluorescence spectroscopy and complexes of 5'-dye-DNA conjugates with bovine serum albumin as probes. In the presence of a single stranded DNA-binding protein the complexes with bovine serum albumin are disrupted, which results in a reduction of fluorescence intensity.
Anorganisch-Chemisches Institut, Ruprecht-Karls-Universität Heidelberg, Heidelberg, Germany.
Annabelle
A
Fülöp
Dumitru
D
Arian
Andriy
A
Lysenko
Andriy
A
Mokhir
D000818:Animals; D002417:Cattle; D004277:DNA, Single-Stranded / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000378:metabolism*; D005456:Fluorescent Dyes / Q000737:chemistry*; D009281:Naphthalenes / Q000737:chemistry; D011485:Protein Binding; D012710:Serum Albumin, Bovine / Q000378:metabolism*; D013050:Spectrometry, Fluorescence / Q000379:methods*
D004277:DNA, Single-Stranded; D004268:DNA-Binding Proteins; D005456:Fluorescent Dyes; D009281:Naphthalenes; D012710:Serum Albumin, Bovine