A novel porcine gene--erlin2, differentially expressed in the liver tissues from Meishan and Large White pigs.
The messenger RNA differential display technique was performed to investigate the differences of gene expression in the liver tissues from Meishan and Large White pigs. A fragment of one differentially expressed gene was isolated and sequenced. A complete complementary DNA (cDNA) sequence was obtained using the rapid amplification of cDNA end method. Nucleotide sequence of the gene is not homologous to any of the known porcine genes. The sequence prediction analysis revealed that the open reading frame of this gene encodes a protein of 339 amino acids which have high homology with those of the ER lipid-raft-associated 2 isoform 2 (ERLIN2) of eight species--human (97%), rhesus monkey (97%), rat (96%), horse (97%), cattle (97%), mouse (97%), dog (95%), and red jungle fowl (90%)--so that it can be defined as the swine erlin2 gene. The phylogenetic tree analysis revealed that the swine erlin2 gene has a closer genetic relationship with the erlin2 genes of human and rhesus monkey. The tissue expression profile analysis indicated that the swine erlin2 gene is differentially expressed in detected tissues from Meishan and Large White pigs. Our experiment suggested that the swine erlin2 gene might play an important role in the superabundant fat deposition of Chinese pigs.
College of Animal Sciences and Technology, Yunnan Agricultural University, Kunming 650201, China. liuyg4567@163.com
Liu
L
Yonggang
D000273:Adipose Tissue / Q000254:growth & development; D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D018076:DNA, Complementary / Q000737:chemistry; D004285:Dogs; D005260:Female; D015870:Gene Expression; D006801:Humans; D008099:Liver / Q000378:metabolism; D008253:Macaca mulatta / Q000235:genetics; D008297:Male; D021962:Membrane Microdomains / Q000737:chemistry* / Q000235:genetics; D008565:Membrane Proteins / Q000737:chemistry* / Q000235:genetics; D051379:Mice; D008969:Molecular Sequence Data; D021141:Nucleic Acid Amplification Techniques; D010802:Phylogeny; D051381:Rats; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment; D013045:Species Specificity; D034421:Sus scrofa / Q000235:genetics*
D018076:DNA, Complementary; C121703:ERLIN2 protein, human; D008565:Membrane Proteins