Pomegranate flower ameliorates fatty liver in an animal model of type 2 diabetes and obesity.
OBJECTIVE
Fatty liver is the most common cause of abnormal liver function tests. We investigated the effect and its underlying mechanism of pomegranate flower (PGF), a traditional antidiabetic medicine, on fatty liver.


METHODS
At the endpoint of treatment of male Zucker diabetic fatty (ZDF) rats with PGF extract (500 mg/kg, p.o. x 6 weeks), liver weight index, hepatic lipid contents (enzymatic colorimetric methods) and droplet accumulation (Oil Red O staining) were determined. Gene profiles (RT-PCR) were analyzed in the liver of ZDF rats and in human liver-derived HepG2 cell line.


RESULTS
PGF-treated ZDF rats showed reduced ratio of liver weight to tibia length, hepatic triglyceride contents and lipid droplets. These effects were accompanied by enhanced hepatic gene expression of peroxisome proliferator-activated receptor (PPAR)-alpha, carnitine palmitoyltransferase-1 and acyl-CoA oxidase (ACO), and reduced stearoyl-CoA desaturase-1. In contrast, PGF showed minimal effects on expression of genes responsible for synthesis, hydrolysis or uptake of fatty acid and triglycerides. PGF treatment also increased PPAR-alpha and ACO mRNA levels in HepG2 cells.


CONCLUSIONS
Our findings suggest that this Unani medicine ameliorates diabetes and obesity-associated fatty liver, at least in part, by activating hepatic expression of genes responsible for fatty acid oxidation.
Faculty of Pharmacy, The University of Sydney, Sydney, NSW 2006, Australia.
Kevin Zhe-Yang
KZ
Xu
Chenchen
C
Zhu
Moon Sun
MS
Kim
Johji
J
Yamahara
Yuhao
Y
Li
D000818:Animals; D006528:Carcinoma, Hepatocellular / Q000378:metabolism; D045744:Cell Line, Tumor; D003921:Diabetes Mellitus, Experimental / Q000150:complications; D003924:Diabetes Mellitus, Type 2 / Q000150:complications*; D005234:Fatty Liver / Q000209:etiology / Q000235:genetics / Q000517:prevention & control*; D035264:Flowers; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D008111:Liver Function Tests; D008113:Liver Neoplasms / Q000378:metabolism; D029561:Lythraceae / Q000737:chemistry*; D008297:Male; D009765:Obesity / Q000150:complications; D047493:PPAR alpha / Q000187:drug effects / Q000235:genetics; D010936:Plant Extracts / Q000494:pharmacology*; D012333:RNA, Messenger / Q000187:drug effects / Q000378:metabolism; D051381:Rats; D011924:Rats, Zucker
D047493:PPAR alpha; D010936:Plant Extracts; D012333:RNA, Messenger