LPS induces GROalpha chemokine production via NF-kappaB in oral fibroblasts.
OBJECTIVE
Chemotaxis of neutrophils from blood to the inflammation process plays an important role in development of periodontal inflammation. The novel chemokine GROalpha, also named CXCL1, is a strong chemoattractant for neutrophils. Data on production and regulation of GROalpha by oral fibroblasts have not previously been presented.


METHODS
GROalpha mRNA and protein levels were determined in human periodontal ligament cells and mouse gingival fibroblasts by quantitative real-time PCR and ELISA.


RESULTS
We disclose that both human periodontal ligament cells and mouse gingival fibroblasts produce GROalpha in response to LPS stimulation. Stimulation with LPS for 24 h increased both mRNA for GROalpha and GROalpha protein. The steroid hormone estrogen had no effect on LPS-induced GROalpha mRNA expression. Treatment with the glucocorticoid dexamethasone attenuated LPS-induced GROalpha production, and the NF-kappaB blocker MG 132 fully prevented LPS-induced GROalpha.


CONCLUSIONS
Oral fibroblasts respond to LPS stimulation by increasing GROalpha production via the transcription factor NF-kappaB, suggesting that this mechanism may be involved in development of periodontal inflammation.
Jönsson
D
D
Department of Experimental Medical Science, Faculty of Medicine, Lund University, Lund, Sweden.
Amisten
S
S
Bratthall
G
G
Holm
A
A
Nilsson
Bengt-Olof
BO
D000293:Adolescent; D000818:Animals; D054360:Chemokine CXCL1 / Q000096:biosynthesis* / Q000235:genetics / Q000276:immunology*; D002630:Chemotactic Factors / Q000096:biosynthesis / Q000276:immunology; D002634:Chemotaxis, Leukocyte / Q000502:physiology; D002648:Child; D005260:Female; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects* / Q000276:immunology*; D005881:Gingiva / Q000166:cytology; D006801:Humans; D008070:Lipopolysaccharides / Q000276:immunology / Q000494:pharmacology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D016328:NF-kappa B / Q000378:metabolism*; D009504:Neutrophils / Q000276:immunology / Q000378:metabolism; D010513:Periodontal Ligament / Q000166:cytology
D054360:Chemokine CXCL1; D002630:Chemotactic Factors; D008070:Lipopolysaccharides; D016328:NF-kappa B