Protective immunity against Toxoplasma challenge in mice by coadministration of T. gondii antigens and Eimeria profilin-like protein as an adjuvant.
Toll-like receptor (TLR) ligands are attractive adjuvant candidates in vaccine development. Eimeria tenella profilin-like protein has recently been shown to be a potent agonist of the innate immune system through its recognition by Toll-like receptor-11. In this report, we studied the systemic and mucosal adjuvant activity of Eimeria profilin-like protein within a vaccinal strategy against Toxoplasma gondii in mice. Using intraperitoneal (i.p.) immunization, we observed that coadministration of the recombinant Eimeria antigen (rEA) with T. gondii antigen (TAg) effectively elevates plasma levels of IL-12p70 and consequently induced both enhanced specific humoral and Th1 cellular immune responses. The co-administration of TAg plus rEA by i.p route significantly enhanced the protection against T. gondii infection (62% brain cyst reduction) in comparison with control mice and with mice immunized with TAg alone (only 36% brain cyst reduction). After intranasal immunization, humoral and cellular responses were weak. However mice immunized nasally with TAg plus rEA were significantly protected with 50% of brain cyst reduction, conversely TAg immunized mice did not present any brain cyst reduction.These results indicate that Eimeria profilin-like protein would serve as an efficacious systemic and mucosal adjuvant inducing protective immune response against chronical stage of T. gondii infection through TLR11 activation.
UMR 483 Université-INRA d'Immunologie Parasitaire, Vaccinologie et Biothérapie anti-infectieuse, IFR 136 Agents transmissibles et Infectiologie, UFR des Sciences Pharmaceutiques, Tours, France.
Dorsaf
D
Hedhli
Isabelle
I
Dimier-Poisson
John W
JW
Judge
Barnett
B
Rosenberg
Marie Noëlle
MN
Mévélec
D000276:Adjuvants, Immunologic / Q000008:administration & dosage*; D000281:Administration, Intranasal; D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood; D000953:Antigens, Protozoan / Q000008:administration & dosage* / Q000276:immunology; D016207:Cytokines / Q000096:biosynthesis; D004539:Eimeria; D005260:Female; D007114:Immunization; D007074:Immunoglobulin G / Q000145:classification; D007274:Injections, Intraperitoneal; D051379:Mice; D008808:Mice, Inbred CBA; D051304:Profilins; D015800:Protozoan Proteins / Q000008:administration & dosage*; D016052:Protozoan Vaccines / Q000008:administration & dosage / Q000276:immunology*; D051193:Toll-Like Receptors / Q000819:agonists*; D014122:Toxoplasma / Q000276:immunology*
D000276:Adjuvants, Immunologic; D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D016207:Cytokines; D007074:Immunoglobulin G; D051304:Profilins; D015800:Protozoan Proteins; D016052:Protozoan Vaccines; C482903:Tlr11 protein, mouse; D051193:Toll-Like Receptors