Delivery rate using cryopreserved oocytes is comparable to conventional in vitro fertilization using fresh oocytes: potential fertility preservation for female cancer patients.
OBJECTIVE
To explore the use of oocyte cryopreservation as a fertility-conserving option. Cancer treatments administered during the reproductive and adolescent years can result in sterility. Previous fertility preservation efforts focused on embryo rather than oocyte storage because the latter was deemed inefficient. Recently, several large reports of healthy births resulting from the transfer of embryos derived from frozen/thawed oocytes have been published. We sought to establish an oocyte cryopreservation program at our center.


METHODS
Twenty-three oocyte cryopreservation cycles were performed. Collected oocytes were cryopreserved by either the slow or the vitrification method. Approximately 1-4 months later, a programmed cycle of thawing/warming, fertilization with intracytoplasmic sperm injection, and ET was performed; cycle and pregnancy outcomes were assessed.


METHODS
University-based fertility center.


METHODS
Twenty-two infertile women.


METHODS
Oocyte cryopreservation.


METHODS
Oocyte survival, embryo development, pregnancy outcomes.


RESULTS
Oocyte survival, 2-pronuclei fertilization, and blastocyst formation rates were 92%, 79%, and 43%, respectively. Fourteen women became pregnant; one miscarried; 10 have delivered 13 viable infants, and three pregnancies are ongoing for an ongoing/delivered pregnancy rate of 57%. This result was not statistically different from cycles performed consecutively in age-matched controls using fresh, nonfrozen autologous or donor oocytes during a similar time period.


CONCLUSIONS
Oocyte cryopreservation appears to be a viable option for fertility preservation in some centers.
Grifo
James A
JA
New York University Fertility Center, New York University School of Medicine, New York, New York, USA.
Noyes
Nicole
N
D000293:Adolescent; D000328:Adult; D001755:Blastocyst / Q000502:physiology; D002470:Cell Survival; D036861:Delivery, Obstetric / Q000706:statistics & numerical data*; D010064:Embryo Implantation; D004624:Embryo Transfer / Q000706:statistics & numerical data; D004958:Estradiol / Q000097:blood; D005260:Female; D005307:Fertilization in Vitro / Q000379:methods / Q000706:statistics & numerical data*; D005640:Follicle Stimulating Hormone / Q000097:blood; D005615:Freezing; D005833:Genital Neoplasms, Female / Q000503:physiopathology*; D006801:Humans; D054315:Oocyte Retrieval / Q000379:methods; D009865:Oocytes / Q000166:cytology; D011247:Pregnancy; D011256:Pregnancy Outcome; D018873:Pregnancy Rate
D004958:Estradiol; D005640:Follicle Stimulating Hormone