Possible contribution of hyalocytes to idiopathic epiretinal membrane formation and its contraction.
OBJECTIVE
To address the cellular components and the contractile mechanisms of the idiopathic epiretinal membrane (ERM).


METHODS
Ten surgically removed ERMs were fixed in 4% paraformaldehyde and analysed by whole-mount immunohistochemistry with anti-glial fibrillar acidic protein (GFAP) and alpha smooth-muscle actin (alphaSMA) antibodies. Type I collagen gel contraction assay, an established wound-healing assay in vitro, was performed using cultured bovine hyalocytes or normal human astrocytes (NHA) to evaluate the contractile property of the cells in the presence of tissue growth factor (TGF)-beta2. The expression of alphaSMA was also analysed by western blot analysis to examine myofibroblastic transdifferentiation of the cells. Vitreous-induced collagen gel contraction was also evaluated.


RESULTS
All membranes were composed of alphaSMA immunopositive cells in contracted foci and GFAP immunopositive cells in the periphery. No apparent double positive cells were observed in any membranes examined. Cultured hyalocytes showed overexpression of alphaSMA and hypercontraction of collagen gels in response to TGF-beta2, while glial cells showed marginal change. The vitreous from ERM patients also caused overexpression of alphaSMA and hypercontraction of the gels embedding hyalocytes, which were almost completely inhibited in the presence of anti-TGF-beta2 neutralising antibody.


CONCLUSIONS
Hyalocytes might be one of the critical components of ERM mediating its contractile property through the effect of TGF-beta2 in the vitreous fluid.
Kohno
R-i
RI
Department of Ophthalmology, Graduate School of Medical Sciences, Kyushu University, 3-1-1, Maidashi, Higashi-ku, Fukuoka 812-8582, Japan.
Hata
Y
Y
Kawahara
S
S
Kita
T
T
Arita
R
R
Mochizuki
Y
Y
Aiello
L P
LP
Ishibashi
T
T
D000199:Actins / Q000378:metabolism; D000368:Aged; D000818:Animals; D001253:Astrocytes / Q000648:ultrastructure; D002417:Cattle; D002478:Cells, Cultured; D003094:Collagen / Q000378:metabolism; D019773:Epiretinal Membrane / Q000209:etiology / Q000378:metabolism / Q000473:pathology*; D005260:Female; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D006801:Humans; D008297:Male; D008854:Microscopy, Electron; D008875:Middle Aged; D053781:Transforming Growth Factor beta2 / Q000494:pharmacology / Q000502:physiology; D014822:Vitreous Body / Q000187:drug effects / Q000648:ultrastructure*
D000199:Actins; D005904:Glial Fibrillary Acidic Protein; D053781:Transforming Growth Factor beta2; D003094:Collagen