Surface antigen phenotypes of hematopoietic stem cells from embryos and murine embryonic stem cells.
Surface antigens on hematopoietic stem cells (HSCs) enable prospective isolation and characterization. Here, we compare the cell-surface phenotype of hematopoietic repopulating cells from murine yolk sac, aorta-gonad-mesonephros, placenta, fetal liver, and bone marrow with that of HSCs derived from the in vitro differentiation of murine embryonic stem cells (ESC-HSCs). Whereas c-Kit marks all HSC populations, CD41, CD45, CD34, and CD150 were developmentally regulated: the earliest embryonic HSCs express CD41 and CD34 and lack CD45 and CD150, whereas more mature HSCs lack CD41 and CD34 and express CD45 and CD150. ESC-HSCs express CD41 and CD150, lack CD34, and are heterogeneous for CD45. Finally, although CD48 was absent from all in vivo HSCs examined, ESC-HSCs were heterogeneous for the expression of this molecule. This unique phenotype signifies a developmentally immature population of cells with features of both primitive and mature HSC. The prospective fractionation of ESC-HSCs will facilitate studies of HSC maturation essential for normal functional engraftment in irradiated adults.
Division of Pediatric Hematology/Oncology, Manton Center for Orphan Diseases, Stem Cell Transplantation Program, Children's Hospital, Boston, MA 02115, USA.
Shannon L
SL
McKinney-Freeman
Olaia
O
Naveiras
Frank
F
Yates
Sabine
S
Loewer
Marsha
M
Philitas
Matthew
M
Curran
Peter J
PJ
Park
George Q
GQ
Daley
D000818:Animals; D015703:Antigens, CD / Q000032:analysis* / Q000378:metabolism; D002478:Cells, Cultured; D004622:Embryo, Mammalian / Q000378:metabolism; D053595:Embryonic Stem Cells / Q000378:metabolism*; D005260:Female; D006412:Hematopoietic Stem Cells / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D010641:Phenotype; D010920:Placenta / Q000378:metabolism
D015703:Antigens, CD