Single-molecule study on intermolecular interaction between C60 and porphyrin derivatives: toward understanding the strength of the multivalency.
Multivalency is important in molecular self-assembly, although it remains a challenge to correlate the single-molecule results with the multivalent interaction. As the first example, we have combined a well-defined self-assembling system with AFM-based single-molecule force spectroscopy (SMFS) to investigate the intermolecular interactions and multivalency between C(60) and porphyrin derivatives. Compared with the interaction between C(60) and a single porphyrin (29 pN), SMFS has revealed that porphyrin tweezers can provide an enhanced binding interaction with C(60), resulting in a more than 2-fold higher unbinding force (75 pN, at the same loading rate). In addition, a much lower dissociation rate and a shorter effective distance between the bound state and transition state of the interaction are indicated by dynamic force spectroscopy. These results provide new quantitative information on the divalency effect in the unbinding process of C(60) and porphyrin tweezers at the single-molecule level, which is of significance in understanding the strength of the multivalency in the molecular assembly.
Key Lab of Organic Optoelectronics and Molecular Engineering, Department of Chemistry, Tsinghua University, Beijing 100084, PR China.
Yiheng
Y
Zhang
Ying
Y
Yu
Zhenhua
Z
Jiang
Huaping
H
Xu
Zhiqiang
Z
Wang
Xi
X
Zhang
Masafumi
M
Oda
Tomoya
T
Ishizuka
Donglin
D
Jiang
Lifeng
L
Chi
Harald
H
Fuchs