Isolation, culture and characterization of adult carotid body-derived cells.
Recent studies indicate that carotid body (CB) could be a suitable cell source for cell therapy in Parkinson's disease. We have isolated and successfully expanded in culture as monolayer adult CB-derived cells using a modification of the culture medium employed for bone marrow multipotent adult progenitor cells (MAPCs). These cells express variable amounts of tyrosine hydroxylase (TH), beta-III tubulin and Sox2. In addition, CB-derived cells showed high expression of Sox2 related to a high rate of proliferation and consistent with an undifferentiated state. Under culture conditions that reduced cell proliferation, Sox2 expression decreased while TH and beta-III tubulin expression was increased. This could indicate that the differentiation of some cells occurs in the culture, thus accounting for a certain neural differentiation potential of CB-derived cells.
Izal-Azcárate
Amaya
A
Laboratory of Regenerative Therapy, Department of Neuroscience, Centre for Applied Medical Research, University of Navarra, Pamplona, Spain.
Gutiérrez-Pérez
María
M
Izal
Iñigo
I
Belzunegui
Silvia
S
Sebastián
Waldy San
WS
López
Berta
B
Marcilla
Irene
I
Prósper
Felipe
F
Luquin
Maria-Rosario
MR
D000818:Animals; D015153:Blotting, Western; D002344:Carotid Body / Q000166:cytology* / Q000378:metabolism; D018929:Cell Culture Techniques / Q000379:methods*; D002454:Cell Differentiation; D049109:Cell Proliferation; D008297:Male; D009474:Neurons / Q000166:cytology* / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D013234:Stem Cells / Q000166:cytology* / Q000378:metabolism