Design, synthesis, and characterization of fluorescent cobalamin analogues with high quantum efficiencies.
Cobalamin tethered to fluorescein or Rhodamine 6G has been synthesized and characterized. The fluorophore is conjugated to the ribose-5'-OH of cobalamin through a rigid linker to prevent the fluorophore from folding back through space and interacting with the corrin ring of cobalamin. This increases the fluorescence quantum yield. This new family of cobalamin analogues may be suitable for use as tumor markers to tag cancer cells for surgical resection.
Lee
Manfai
M
Department of Chemistry, University of Utah, 315 South 1400 East, Salt Lake City, Utah 84112-0850, USA.
Grissom
Charles B
CB
D014408:Biomarkers, Tumor / Q000138:chemical synthesis* / Q000737:chemistry; D045744:Cell Line, Tumor / Q000737:chemistry / Q000378:metabolism; D003510:Cyclohexanes / Q000737:chemistry*; D004247:DNA / Q000737:chemistry / Q000378:metabolism; D015195:Drug Design; D005456:Fluorescent Dyes / Q000138:chemical synthesis* / Q000737:chemistry; D006801:Humans; D007202:Indicators and Reagents; D015394:Molecular Structure; D011789:Quantum Theory; D012235:Rhodamines / Q000737:chemistry; D014805:Vitamin B 12 / Q000031:analogs & derivatives* / Q000138:chemical synthesis* / Q000737:chemistry
D014408:Biomarkers, Tumor; D003510:Cyclohexanes; D005456:Fluorescent Dyes; D007202:Indicators and Reagents; D012235:Rhodamines; C026188:rhodamine 6G; D004247:DNA; D014805:Vitamin B 12