[Small interfering RNA targeting NF-kappaB Inhibits human umbilical venous endothelial cells migration].
OBJECTIVE
To investigate the effect of small interfering RNA(siRNA) targeting NF-kappaB on migration ability of human umbilical venous endothelial cells.


METHODS
EAhy926 cells (Human umbilical vein endothelial cell line) were cultured with stimulation of vascular endothelial growth factor (VEGF), and then were transfected with NF-kappaB -siRNA (40 pmol/L) by using catiotic liposome lipofectamine(TM); 2000 as the transfecting agent. Cells transfected with negative control-siRNA (40 pmol/L), empty Liposome and nothing as control. Forty-eight hours later, cells were collected and prepared for RT-PCR, Western blot analysis, and determination of the migration inhibition rate.


RESULTS
Compared with normal control group, both mRNA and protein levels of NF-kappaB were decreased obviously and at the same time cell migration inhibition rate was increased obviously in NF-kappaB-siRNA group. There were no significant differences between empty liposome group, negative control siRNA group and normal control group, respectively (P>0.05).


CONCLUSIONS
Synthetic siRNA can effectively inhibit endothelial cell migration ability by means of blocking the signaling pathway of NF-kappaB. And siRNA targeting NF-kappaB may be useful for antiangiogenesis treatment.
Department of Obstetrics and Gynecology, Zhujiang Hospital, Southern Medical University, Guangzhou, China. liumb1972@126.com
Mu-biao
MB
Liu
Yuan-li
YL
He
Jie
J
Zhong
D015153:Blotting, Western; D002460:Cell Line; D002465:Cell Movement; D042783:Endothelial Cells / Q000166:cytology / Q000378:metabolism*; D006801:Humans; D016328:NF-kappa B / Q000235:genetics* / Q000378:metabolism; D034741:RNA, Small Interfering / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D051996:Transcription Factor RelA / Q000235:genetics / Q000378:metabolism; D014162:Transfection
D016328:NF-kappa B; D034741:RNA, Small Interfering; D051996:Transcription Factor RelA