SATB1 binds an intronic MAR sequence in human PI3kgamma in vitro.
In our previous study, an intronic MAR sequence in human PI3Kgamma gene (PIMAR) was identified using bioinformatics and biochemical methods. We used MatInspector software to identify potential binding sites for MAR-binding proteins in PIMAR. In this study, a tissue-specific MAR-binding protein (SATB1) was used to characterize the potential binding sites. Southwestern blot analysis indicates that recombinant SATB1 directly binds PIMAR sequence in vitro. Reporter gene assay showed that overexpression of SATB1 downregulates the luciferase reporter linked with reversed PIMAR by approximately threefold in the NIH-3T3 cell line. These results indicate that SATB1 may play antagonistic roles in PI3Kgamma transcriptional regulation.
Department of Biochemistry and Molecular Biology, Institute of Medical Sciences, Shanghai Jiao Tong University School of Medicine, Shanghai, People's Republic of China.
Rong
R
Cai
Weirong
W
Xu
Bingbing
B
Dai
Xia
X
Cai
Rongting
R
Xu
Jian
J
Lu
D000818:Animals; D001665:Binding Sites / Q000235:genetics; D026501:Blotting, Southwestern; D058544:Class Ib Phosphatidylinositol 3-Kinase; D005786:Gene Expression Regulation / Q000235:genetics*; D006801:Humans; D066298:In Vitro Techniques; D007438:Introns / Q000235:genetics*; D007527:Isoenzymes / Q000235:genetics / Q000378:metabolism; D008156:Luciferases / Q000378:metabolism; D036961:Matrix Attachment Region Binding Proteins / Q000235:genetics / Q000378:metabolism*; D045170:Matrix Attachment Regions / Q000235:genetics*; D051379:Mice; D041681:NIH 3T3 Cells; D019869:Phosphatidylinositol 3-Kinases / Q000235:genetics / Q000378:metabolism*; D010957:Plasmids / Q000235:genetics
D007527:Isoenzymes; D036961:Matrix Attachment Region Binding Proteins; C076189:SATB1 protein, human; D008156:Luciferases; D019869:Phosphatidylinositol 3-Kinases; D058544:Class Ib Phosphatidylinositol 3-Kinase; C546760:PIK3CG protein, human; C546764:Pik3cg protein, mouse