Protective effect of lipoic acid against acrolein-induced cytotoxicity in IMR-90 human fibroblasts.
Acrolein is a highly reactive unsaturated hazardous air pollutant of human health concern, particularly as a component of cigarette smoke. In this study, the effects of acrolein on mitochondrial damage in IMR-90 (a human lung fibroblast cell line), and the reduction of this damage by R-alpha-lipoic acid were examined. Our results show that acute acrolein exposure exceeding 100 microM (24 h) in IMR-90 cells caused serious cytotoxicity, including decreases in cell viability, mitochondrial membrane potential, SOD activity, GSH and ATP levels, and acute exposure also increased in ROS levels. Pretreatment with R-alpha-lipoic acid effectively protected IMR-90 cells from acrolein toxicity. The results show that acrolein is a mitochondrial toxin in IMR-90 cells and that acrolein-induced oxidative mitochondrial dysfunction is reduced by R-alpha-lipoic acid. These experiments imply R-alpha-lipoic acid may be an effective antioxidant for reducing or preventing chronic oxidant-induced lung cells degeneration in vivo from a variety of sources, including cigarette smoke.
Department of Nutrition and Food Hygiene, School of Public Health, China Medical University, Shenyang, China. lhjia@mail.cmu.edu.cn
Lihong
L
Jia
Zhiyu
Z
Zhang
Lingling
L
Zhai
Yinglong
Y
Bai
D000171:Acrolein / Q000633:toxicity*; D000255:Adenosine Triphosphate / Q000378:metabolism; D000393:Air Pollutants / Q000633:toxicity; D000975:Antioxidants / Q000494:pharmacology*; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D003603:Cytotoxins / Q000494:pharmacology; D005347:Fibroblasts / Q000187:drug effects* / Q000378:metabolism; D005978:Glutathione / Q000378:metabolism; D006801:Humans; D019570:Inhalation Exposure / Q000009:adverse effects*; D008168:Lung / Q000187:drug effects* / Q000378:metabolism; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D008928:Mitochondria / Q000187:drug effects*; D028361:Mitochondrial Diseases / Q000139:chemically induced; D017382:Reactive Oxygen Species / Q000378:metabolism; D012906:Smoke / Q000009:adverse effects; D013482:Superoxide Dismutase / Q000378:metabolism; D008063:Thioctic Acid / Q000494:pharmacology*
D000393:Air Pollutants; D000975:Antioxidants; D003603:Cytotoxins; D017382:Reactive Oxygen Species; D012906:Smoke; D008063:Thioctic Acid; D000171:Acrolein; D000255:Adenosine Triphosphate; D013482:Superoxide Dismutase; D005978:Glutathione