A heat-activated and thermoresistant telomerase activity in Leishmania major Friedlin.
Here we studied the telomerase activity of the human parasite Leishmania major. In this organism we have detected a high activity of this enzyme once several parameters such as heat activation, sequence of extension primer, and protein concentration are adjusted. The activity was not only heat activated, but also very resistant to heat denaturation. We believe L. major telomerase is an important activity and it may provide an adequate drug therapy target.
Galindo
Maria Mercedes
MM
Centro de Biotecnología, Instituto de Estudios Avanzados, Ministerio del poder Popular para la Ciencia y Tecnología, Carretera Nacional Hoyo de la Puerta, Caracas, Venezuela.
Rodriguez
Evelyn
E
Rojas
Maria Gabriela
MG
Figarella
Katherine
K
Campelo
Riward
R
Ramírez
Jose Luis
JL
D000818:Animals; D006358:Hot Temperature; D018320:Leishmania major / Q000201:enzymology* / Q000528:radiation effects*; D011489:Protein Denaturation / Q000528:radiation effects; D015800:Protozoan Proteins / Q000378:metabolism*; D019098:Telomerase / Q000378:metabolism*
D015800:Protozoan Proteins; D019098:Telomerase