Isolation and characterization of plant growth-promoting strain Pantoea NII-186. From Western Ghat forest soil, India.
OBJECTIVE
To isolate plant growth-promoting bacterium from Western Ghat forests in India.


RESULTS
A Gram-negative, rod shaped, cream white coloured strain Pantoea NII-186 isolated from Western Ghat soil sample. The taxonomic position of the bacterium was confirmed by sequencing of 16S rRNA and phylogenetic analysis. A strain grew at a wide range of temperature ranging from 5-40 degrees C, but optimum growth was observed at 28-30 degrees C. It showed multiple plant growth-promoting attributes such as phosphate solubilization activity, indole acetic acid (IAA) production, siderophore production and HCN production. It was able to solubilize (28 microg of Ca(3)PO(4) ml(-1) day(-1)), and produce IAA (59 microg) at 28 degrees C. The solubilization of insoluble phosphate was associates with a drop in the pH of the culture medium. Pantoea sp. NII-186 tolerate to different environmental stresses like 5-40 degrees C, 0-7% salt concentration and 4-12 pH range.


CONCLUSIONS
The 16S rRNA gene sequence confirmed that the isolate NII-186 was belongs to Pantoea genus and showed considerable differences in physiological properties with previously reported species of this genus. Isolate NII-186 possessed multiple attributes of plant growth-promoting activity.


CONCLUSIONS
Hence in the context it is proposed that Pantoea sp. NII-186, could be deployed as an inoculant to attain the desired plant growth-promoting activity in agricultural environment.
Dastager
S G
SG
Biotechnology Division, National Institute for Interdisciplinary Science and Technology (CSIR), Trivandrum, India.
Deepa
C K
CK
Puneet
S C
SC
Nautiyal
C S
CS
Pandey
A
A
D000900:Anti-Bacterial Agents / Q000494:pharmacology; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D006856:Hydrogen Cyanide / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D007194:India; D008969:Molecular Sequence Data; D020636:Pantoea / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology*; D010710:Phosphates / Q000378:metabolism; D010802:Phylogeny; D010937:Plant Growth Regulators / Q000096:biosynthesis*; D010944:Plants; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D013696:Temperature; D014197:Trees
D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D010710:Phosphates; D010937:Plant Growth Regulators; D012336:RNA, Ribosomal, 16S; D006856:Hydrogen Cyanide