Enzymatic hydrolysis of T-2 toxin for the quantitative determination of total T-2 and HT-2 toxins in cereals.
The selective enzymatic deacetylation of T-2 toxin to give HT-2 toxin has been investigated in aqueous crude extracts of different cereals and exploited to develop an analytical method for the determination of the sum of T-2 and HT-2 toxins. The method has been validated for the analysis of total T-2 and HT-2 toxins in maize, wheat, and oats, showing recoveries from 72 to 97% for maize, from 67 to 84% for wheat, and from 61% to 87% for oats, at spiking levels of 20-400 microg/kg, with relative standard deviation lower than 10%. Liquid chromatography-tandem mass spectrometry was used for quantitative toxin determination. The potential biological role of this enzymatic conversion and its perspectives for application in the development of antibody-based analytical techniques are discussed.
Lattanzio
Veronica M T
VM
Institute of Sciences of Food Production (ISPA), National Research Council (CNR), Via Amendola 122/o, Bari 70126, Italy. veronica.lattanzio@ispa.cnr.it
Solfrizzo
Michele
M
Visconti
Angelo
A
D043182:Carboxylesterase / Q000378:metabolism*; D002853:Chromatography, Liquid / Q000379:methods*; D002523:Edible Grain / Q000737:chemistry* / Q000201:enzymology*; D015394:Molecular Structure; D013605:T-2 Toxin / Q000031:analogs & derivatives* / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D053719:Tandem Mass Spectrometry / Q000379:methods*
D043182:Carboxylesterase; D013605:T-2 Toxin; C012351:HT-2 toxin