Gene expression profiling in rat liver after VMH lesioning.
It has been reported that ventromedial hypothalamic (VMH) lesions induce hepatic cell proliferation and apoptosis and metabolic changes in the body. In the present study, we identified genes of which expression profiles showed significant modulation in rat liver after VMH lesions. Total RNA was extracted, and differences in the gene expression profiles between rats at day 3 after VMH lesioning and sham-VMH lesioned rats were investigated using DNA microarray analysis. The results revealed that VMH lesions regulated the genes that were involved in various types of metabolisms and cell proliferations in the liver. Real-time PCR also confirmed that gene expressions of ELOVL6 and SPC24 were upregulated, and that of SERPINA7 was downregulated. VMH lesions may change the expressions of multiple metabolism genes and cell proliferation-related genes in rat liver.
Laboratory of Clinical Nutrition & Physiology, Kyoritsu Women's University, Tokyo 101-8433, Japan. takkiba@hotmail.com
Takayoshi
T
Kiba
Yuri
Y
Kintaka
Yoko
Y
Suzuki
Eiko
E
Nakata
Yasuhito
Y
Ishigaki
Shuji
S
Inoue
D000123:Acetyltransferases / Q000235:genetics / Q000378:metabolism; D000818:Animals; D017209:Apoptosis; D049109:Cell Proliferation; D004195:Disease Models, Animal; D015536:Down-Regulation; D000081031:Fatty Acid Elongases; D005260:Female; D020869:Gene Expression Profiling; D008099:Liver / Q000378:metabolism* / Q000473:pathology*; D008869:Microtubule-Associated Proteins / Q000235:genetics / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis; D051381:Rats; D017207:Rats, Sprague-Dawley; D056825:Thyroxine-Binding Globulin; D013975:Thyroxine-Binding Proteins / Q000235:genetics / Q000378:metabolism; D015854:Up-Regulation; D014697:Ventromedial Hypothalamic Nucleus / Q000473:pathology* / Q000601:surgery
D008869:Microtubule-Associated Proteins; C543064:SPC24 protein, rat; C534947:Serpina7 protein, rat; D056825:Thyroxine-Binding Globulin; D013975:Thyroxine-Binding Proteins; D000123:Acetyltransferases; D000081031:Fatty Acid Elongases