Characterization of possible correlates of protective response against Brucella ovis infection in rams immunized with the B. melitensis Rev 1 vaccine.
Vaccination with the live attenuated Brucella melitensis Rev 1 vaccine is used to control ovine brucellosis caused by Brucella ovis in sheep. The objective of this study was to identify possible correlates of protective response to B. ovis infection through the characterization by microarray hybridization and real-time RT-PCR of inflammatory and immune response genes differentially expressed in rams previously immunized with B. melitensis Rev 1 and experimentally challenged with B. ovis. Gene expression profiles were compared before and after challenge with B. ovis between rams protected and those vaccinated but found infected after challenge. The TLR10, Bak and ANXI genes were expressed at higher levels in vaccinated and protected rams. These genes provide possible correlates of protective response to B. ovis infection in rams immunized with the B. melitensis Rev 1 vaccine.
Instituto de Investigación en Recursos Cinegéticos IREC (CSIC-UCLM-JCCM), Ciudad Real, Spain.
Ruth C
RC
Galindo
Pilar M
PM
Muñoz
María J
MJ
de Miguel
Clara M
CM
Marin
José M
JM
Blasco
Christian
C
Gortazar
Katherine M
KM
Kocan
José
J
de la Fuente
D000818:Animals; D017305:Annexin A1 / Q000096:biosynthesis / Q000235:genetics; D001428:Bacterial Vaccines / Q000627:therapeutic use*; D015415:Biomarkers / Q000032:analysis* / Q000378:metabolism; D042061:Brucella ovis / Q000276:immunology*; D002006:Brucellosis / Q000235:genetics / Q000276:immunology / Q000517:prevention & control / Q000662:veterinary*; D015870:Gene Expression; D020869:Gene Expression Profiling; D008297:Male; D012313:RNA / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012756:Sheep; D012757:Sheep Diseases / Q000235:genetics* / Q000276:immunology / Q000517:prevention & control*; D051201:Toll-Like Receptor 10 / Q000096:biosynthesis / Q000235:genetics; D014613:Vaccines, Attenuated / Q000627:therapeutic use; D050998:bcl-2 Homologous Antagonist-Killer Protein / Q000096:biosynthesis / Q000235:genetics
D017305:Annexin A1; D001428:Bacterial Vaccines; D015415:Biomarkers; C471349:Rev.1 vaccine, Brucella melitensis; D051201:Toll-Like Receptor 10; D014613:Vaccines, Attenuated; D050998:bcl-2 Homologous Antagonist-Killer Protein; D012313:RNA