Anomalous quantitation standard growth curves in a laboratory-developed hepatitis C virus (HCV) RNA quantification assay using the TaqMan HCV analyte-specific reagent.
A retrospective examination of quantitation standard growth curves associated with 1,000 unique clinical serum specimens tested by a laboratory-developed TaqMan hepatitis C virus analyte-specific reagent-based assay revealed anomalous growth curves associated with 0.40% (95% confidence interval, 0.11% to 1.00%) of these specimens.
Germer
Jeffrey J
JJ
Division of Clinical Microbiology, Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, MN 55905-0002, USA.
Kathrotiya
Jemee D
JD
Bouse
Carrie F
CF
Mitchell
P Shawn
PS
Yao
Joseph D C
JD
D003951:Diagnostic Errors; D016174:Hepacivirus / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D012313:RNA / Q000235:genetics / Q000302:isolation & purification*; D011933:Reagent Kits, Diagnostic; D019562:Viral Load / Q000379:methods*
D011933:Reagent Kits, Diagnostic; D012313:RNA