Resveratrol ameliorates lung injury via inhibition of apoptosis in rats with severe acute pancreatitis.
The objective of this study was to evaluate the protective effects of resveratrol on lung injury in rats with severe acute pancreatitis. Ninety-six male Sprague-Dawley rats were randomly classified into 4 equal groups (n = 24): control, model, resveratrol-treated, and dexamethasone-treated. The rats were evaluated at 3, 6, and 12 hours after induction of pancreatitis. The following were assessed: P(a)O(2)by arterial blood gas analysis; pancreatic and lung injury by histology; and ultrastructure of lung tissue by transmission electron microscopy. The authors investigated mitochondrial cytochrome c release and evaluated the Bax, Bcl-2, and caspase-3 expression levels in lung tissue over the time course of apoptosis. Changes in lung cell mitochondrial membrane potential were evaluated by confocal laser scanning microscopy. In the model group, lung congestion, edema, inflammatory-cell infiltration, mitochondrial swelling, and cell apoptosis were apparent. In the resveratrol and dexamethasone groups, the morphological changes of the lungs were alleviated. The expression level of Bcl-2 was significantly higher and those of Bax, caspase-3, and cytochrome c were significantly lesser in the resveratrol group than in the model group. Apoptosis is involved in lung injury associated with severe acute pancreatitis, and resveratrol can ameliorate this injury, thus protecting lung function in rats with severe acute pancreatitis.
Department of Hepatobiliary Surgery, First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, China.
Huanchen
H
Sha
Qingyong
Q
Ma
Rajiv Kumar
RK
Jha
Zheng
Z
Wang
D000208:Acute Disease; D000818:Animals; D017209:Apoptosis / Q000187:drug effects* / Q000502:physiology; D003907:Dexamethasone / Q000494:pharmacology; D008168:Lung / Q000648:ultrastructure; D055370:Lung Injury / Q000188:drug therapy* / Q000473:pathology; D051336:Mitochondrial Membranes / Q000473:pathology; D024101:Mitochondrial Proteins / Q000378:metabolism; D010100:Oxygen / Q000097:blood; D010195:Pancreatitis / Q000150:complications*; D051381:Rats; D017207:Rats, Sprague-Dawley; D000077185:Resveratrol; D013267:Stilbenes / Q000494:pharmacology*
D024101:Mitochondrial Proteins; D013267:Stilbenes; D003907:Dexamethasone; D000077185:Resveratrol; D010100:Oxygen