Inhibition of new vessel growth in mouse model of laser-induced choroidal neovascularization by adiponectin peptide II.
We have investigated the effect of adiponectin (APN) peptide II on new vessel growth in mouse model of choroidal neovascularization (CNV) or wet type age-related macular degeneration (AMD). Mice were injected intraperitoneally with APN peptide II, control peptide, or PBS on day 1-7 or day 5-14. APN, AdipoR1, PCNA, and VEGF localization was investigated using confocal microscopy, immunohistochemistry, and RT-PCR. APN peptide II decreased the relative area of FITC-dextran perfused vessels by 4-fold, PCNA expression by 3-fold, and the number of PCNA stained HUVEC and MAVEC cells by 38 and 46%, respectively. We concluded that APN peptide II inhibits CNV size on days 7 and 14 by inhibiting the proliferation of endothelial cells in vivo and in vitro. APN peptide II may have therapeutic potential to inhibit CNV or wet AMD.
Department of Ophthalmology, Jones Eye Institute, Pat & Willard Walker Eye Research Center, University of Arkansas for Medical Sciences, Little Rock, AR 72205, USA.
Valeriy V
VV
Lyzogubov
Ruslana G
RG
Tytarenko
Sushma
S
Thotakura
Tito
T
Viswanathan
Nalini S
NS
Bora
Puran S
PS
Bora
D052242:Adiponectin / Q000737:chemistry / Q000627:therapeutic use*; D000595:Amino Acid Sequence; D000818:Animals; D020256:Choroidal Neovascularization / Q000188:drug therapy* / Q000209:etiology / Q000378:metabolism; D042783:Endothelial Cells / Q000378:metabolism / Q000528:radiation effects; D007834:Lasers; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D023421:Models, Animal; D010446:Peptide Fragments / Q000737:chemistry / Q000627:therapeutic use*; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism / Q000502:physiology; D017434:Protein Structure, Tertiary; D054419:Receptors, Adiponectin / Q000378:metabolism / Q000502:physiology; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism / Q000502:physiology
D052242:Adiponectin; D010446:Peptide Fragments; D018809:Proliferating Cell Nuclear Antigen; D054419:Receptors, Adiponectin; D042461:Vascular Endothelial Growth Factor A