High resolution melting (HRM) analysis for the detection of ER22/23EK, BclI, and N363S polymorphisms of the glucocorticoid receptor gene.
Polymorphisms in the glucocorticoid receptor (GR) gene have been associated with altered sensitivity to glucocorticoids. We designed a high-resolution melting (HRM) assay to detect, simultaneously, the three most intriguing GR polymorphisms, selected on the bases of clinical relevance and frequencies in caucasian population as described in literature. HRM enables the detection of ER22/23EK and N363S genotypes but fails to discriminate homozygous mutant for the BclI polymorphism from wild-type samples, however a simple spike experiment leads to a clear discrimination between these genotypes. The analyses were performed on a cohort of 70 healthy Caucasian subjects. The method was validated by restriction fragment length polymorphisms; HRM results were found to be in 100% concordance with those observed with the restriction enzymes. We also employed this method on a population of 40 Crohn Disease patients; the analysis demonstrated a significantly higher frequency of the BclI polymorphism in patients than in healthy volunteers. This is, at now, the less expensive and time-and work-saving method to detect GR mutations, providing precision, fast screening and high throughput capabilities.
Department of Biomolecular Sciences, University of Urbino, Via Saffi 2, Urbino, Italy. paolo.maltese@uniurb.it
Paolo
P
Maltese
Emanuele
E
Canestrari
Linda
L
Palma
Annamaria
A
Ruzzo
Francesco
F
Corini
Michele
M
Menotta
Francesca
F
Andreoni
Anna
A
Latiano
Vito
V
Annese
Mauro
M
Magnani
D003424:Crohn Disease / Q000188:drug therapy; D004252:DNA Mutational Analysis; D005838:Genotype; D005938:Glucocorticoids / Q000378:metabolism / Q000627:therapeutic use; D006801:Humans; D009691:Nucleic Acid Denaturation; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D011965:Receptors, Glucocorticoid / Q000235:genetics*
D005938:Glucocorticoids; C497713:NR3C1 protein, human; D011965:Receptors, Glucocorticoid