Cyclophilin 40: an Hsp90-cochaperone associated with apo-steroid receptors.
Cyclophilin 40, a divergent loop cyclophilin first identified in association with the estrogen receptor alpha, contains a C-terminal tetratricopeptide repeat domain through which it shares structural identity with FK506-binding protein 52 (FKBP52) and other partner cochaperones in steroid receptor-heat shock protein 90 (Hsp90) complexes. By dynamically competing for Hsp90 interaction, the cochaperones allow the receptors to establish distinct Hsp90-chaperone complexes, with the potential to exert tissue-specific control over receptor activity. Cyclophilin 40 regulates Hsp90 ATPase activity during receptor-Hsp90 assembly. Functional deletion of the cyclophilin 40 yeast homologue, Cpr7, adversely affected estrogen receptor alpha and glucocorticoid receptor activity that could be fully restored, either with wild type Cpr7 or Cpr7 with a cyclophilin domain lacking isomerase activity. We draw parallels with the mechanism already established for FKBP52 and propose that the cyclophilin 40 divergent loop interfaces with a contact surface on the steroid receptor ligand-binding domain to achieve an optimal orientation for receptor activity.
Centre for Medical Research, The University of Western Australia, Nedlands, WA, Australia. tomr@cyllene.uwa.edu.au
Thomas
T
Ratajczak
Bryan K
BK
Ward
Carmel
C
Cluning
Rudi K
RK
Allan
D000595:Amino Acid Sequence; D000818:Animals; D001059:Apoproteins / Q000378:metabolism*; D000081406:Peptidyl-Prolyl Isomerase F; D021983:Cyclophilins / Q000737:chemistry / Q000378:metabolism*; D018841:HSP90 Heat-Shock Proteins / Q000378:metabolism*; D006801:Humans; D008969:Molecular Sequence Data; D021381:Protein Transport; D011987:Receptors, Steroid / Q000378:metabolism*
D001059:Apoproteins; D000081406:Peptidyl-Prolyl Isomerase F; D018841:HSP90 Heat-Shock Proteins; D011987:Receptors, Steroid; D021983:Cyclophilins