Intraportal injection of porcine multipotent mesenchymal stromal cells augments liver regeneration after portal vein embolization.
Portal vein embolization (PVE) can be used prior to liver surgery to increase the volume of the remaining liver tissue after an extensive resection. However, the application of PVE is limited and new strategies to augment liver regeneration by cellular therapy are promising alternatives.


METHODS
The influence of syngeneic multipotent mesenchymal stromal cells (MSC) on liver regeneration was analysed after the ligation of the right portal vein branches in a porcine model, closely mimicking the situation of human surgery. Liver regeneration was monitored by ultrasonography, immunohistological analysis and serum biochemistry.


RESULTS
The volume of the contra-lateral, non-ligated liver lobe increased in all piglets after portal vein ligation. This hyperplasia occurred earlier and was more pronounced in those piglets receiving MSC infusions as compared to non-treated controls. Biochemical liver function was stable in all pigs. Only solitary transplanted MSC were detected in recipient livers two weeks after the infusion.


CONCLUSIONS
The infusion of porcine MSC into the portal vein in a setting of liver regeneration after surgical resection leads to accelerated and augmented hyperplasia. This effect is most likely due to bystander effects of the transplanted MSC.
Liska
Vaclav
V
Department of Surgery, Teaching Hospital and Medical School Pilsen, Charles University Prague, Alej svobody 80, 304 00 Pilsen, Czech Republic. Vena.Liska@seznam.cz
Slowik
Przemyslav
P
Eggenhofer
Elke
E
Treska
Vladislav
V
Renner
Phillip
P
Popp
Felix C
FC
Mirka
Hynek
H
Kobr
Jiri
J
Sykora
Roman
R
Schlitt
Hans-Juergen
HJ
Holubec
Lubos
L
Chlumska
Alena
A
Skalicky
Tomas
T
Matejovic
Martin
M
Dahlke
Marc H
MH
D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology; D049109:Cell Proliferation; D017690:Cell Transplantation / Q000379:methods; D016207:Cytokines / Q000096:biosynthesis; D004621:Embolization, Therapeutic / Q000379:methods*; D007150:Immunohistochemistry / Q000379:methods; D008099:Liver / Q000378:metabolism / Q000473:pathology; D008115:Liver Regeneration; D008648:Mesoderm / Q000166:cytology*; D011169:Portal Vein / Q000473:pathology*; D017154:Stromal Cells / Q000166:cytology*; D013552:Swine; D013997:Time Factors; D014463:Ultrasonography / Q000379:methods
D016207:Cytokines