Changes in total CO2 measurement according to reagent cassette rotation in chemistry autoanalyzers.
The quality control (QC) failure rate in the serum total carbon dioxide (TCO(2)) test increases at a higher rate than in other tests over time after calibration. The causes of the increased QC failure rate in the TCO(2) test were examined. Using a TBA200RF analyzer (Toshiba Medical Systems), the TCO(2) of the QC material was measured at 2-hr intervals and was found to decrease by up to 16.5% at 10 hr after calibration. In contrast, using the P-module and D-module analyzers (Roche Diagnostics), the TCO(2) of the QC material did not change significantly during 10 hr after calibration. When the TCO(2) level of the QC material was measured hourly over 5 hr with the TBA200FR analyzer while the reagent bottle was rotated at 0, 80, 120, 160, or 200 rpm, the rate of decline of TCO(2), increased over time after calibration and with increasing reagent cassette rotation. Therefore, in a clinical laboratory using an automated analyzer with a rotating reagent cassette, it is necessary to set a limit to the calibration time interval in order to satisfy the QC goal.
Department of Laboratory Medicine, University of Ulsan College of Medicine and Asan Medical Center, 388-1 Pungnap-2dong, Songpa-gu, Seoul 138-736, Republic of Korea.
Hee-Jung
HJ
Chung
Woochang
W
Lee
Sail
S
Chun
So Young
SY
Kang
Woo In
WI
Lee
Hae-Il
HI
Park
Won-Ki
WK
Min
D001322:Autoanalysis / Q000379:methods* / Q000592:standards; D001639:Bicarbonates / Q000097:blood*; D002138:Calibration; D002245:Carbon Dioxide / Q000097:blood*; D002254:Carbonates / Q000032:analysis; D002255:Carbonic Acid / Q000032:analysis; D006801:Humans; D007700:Kinetics; D007753:Laboratories / Q000592:standards; D015588:Observer Variation; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012996:Solutions
D001639:Bicarbonates; D002254:Carbonates; D012996:Solutions; D002245:Carbon Dioxide; D002255:Carbonic Acid