Simultaneous imaging of cortical hemodynamics and blood oxygenation change during cerebral ischemia using dual-wavelength laser speckle contrast imaging.
A dual-wavelength laser speckle contrast imaging technique (DW-LSCI) is presented for simultaneous imaging of cerebral blood flow and hemoglobin oxygenation changes at high spatiotemporal resolutions. Experimental validation was performed using a rat transient forebrain ischemia model. The results showed that DW-LSCI was able to track detailed hemodynamic and metabolic changes induced by ischemia, i.e., decreased oxy- and total hemoglobin concentrations and blood flow as well as increased deoxy-hemoglobin concentration in the downstream regions, thus allowing us to distinguish cerebral arterial and venous flows. Simultaneous cerebral blood flow and oxygenation imaging at high spatiotemporal resolutions is crucial to the understanding of neural process and brain functions.
Luo
Zhongchi
Z
Departments of Biomedical Engineering, State University of New York at Stony Brook, Stony Brook, New York 11794-8181, USA.
Yuan
Zhijia
Z
Pan
Yingtian
Y
Du
Congwu
C
D000818:Animals; D001810:Blood Volume; D002545:Brain Ischemia / Q000097:blood* / Q000503:physiopathology; D002540:Cerebral Cortex / Q000098:blood supply* / Q000503:physiopathology; D002560:Cerebrovascular Circulation; D003952:Diagnostic Imaging; D006439:Hemodynamics; D007259:Infrared Rays; D007834:Lasers; D010100:Oxygen / Q000097:blood*; D010108:Oxyhemoglobins / Q000378:metabolism; D051381:Rats; D013997:Time Factors
D010108:Oxyhemoglobins; D010100:Oxygen