Diffusion coefficients of biomolecules using long-lived spin states.
We report the first observation of long-lived states (LLS) having lifetimes T(LLS) that exceed the corresponding spin-lattice relaxation times T(1) by more than a factor 6 in a protein. Slow diffusion coefficients characteristic of large biomolecules can be determined by combining LLS methods with moderate pulsed field gradients (PFGs) available on commercial probeheads, as the extension of spin memory reduces the strain on the duration and/or strength of the PFGs. No isotope labeling of the biomolecule is necessary.
Institut de Sciences et Ingénierie Chimiques, Ecole Polytechnique Fédérale de Lausanne, EPFL, Batochime, 1015 Lausanne, Switzerland.
Puneet
P
Ahuja
Riddhiman
R
Sarkar
Paul R
PR
Vasos
Geoffrey
G
Bodenhausen
D003080:Cold Temperature; D004058:Diffusion; D004151:Dipeptides / Q000737:chemistry*; D008958:Models, Molecular; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods*; D011506:Proteins / Q000737:chemistry*
D004151:Dipeptides; D011506:Proteins; C028872:alanylglycine