The use of laser microdissection and SELDI-TOF MS in ovarian cancer tissue to identify protein profiles.
BACKGROUND
There is a strong need for prognostic biomarkers in ovarian cancer patients due to the heterogeneous responses on current treatment modalities.


METHODS
This study investigates the feasibility of combining laser microdissection (LMD) and surface enhanced laser desorption ionization-time of flight mass spectrometry (SELDI-TOF MS) in ovarian cancer tissue to obtain protein profiles.


RESULTS
Ideal conditions for preparing a protein lysate were determined and subsequently analysed on SELDI-TOF MS. Applying these protocols on tissue of 9 ovarian cancer patients showed different protein profiles between platinum sensitive and resistant patients.


CONCLUSIONS
This shows that combining optimised protocols for LMD with SELDI-TOF MS can be used to obtain discriminatory protein profiles. However, studies with large patient numbers and validation sets are essential to identify reliable biomarkers using this approach.
University Hospitals Leuven, Campus Gasthuisberg, Division of Gynecologic Oncology, Herestraat 49, B-3000 Leuven, Belgium. Isabelle.Cadron@uz.kuleuven.ac.be.
Isabelle
I
Cadron
Toon
T
Van Gorp
Frederic
F
Amant
Ignace
I
Vergote
Philippe
P
Moerman
Etienne
E
Waelkens
Anneleen
A
Daemen
Raf
R
Van De Plas
Bart
B
De Moor
Robert
R
Zeillinger
D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000032:analysis*; D019008:Drug Resistance, Neoplasm; D005260:Female; D006801:Humans; D007834:Lasers; D042282:Microdissection; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000032:analysis*; D009944:Organoplatinum Compounds / Q000627:therapeutic use; D010051:Ovarian Neoplasms / Q000737:chemistry* / Q000188:drug therapy / Q000473:pathology; D040081:Protein Array Analysis / Q000379:methods; D020543:Proteome / Q000032:analysis*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D014408:Biomarkers, Tumor; D009363:Neoplasm Proteins; D009944:Organoplatinum Compounds; D020543:Proteome