Functional analysis of MycE and MycF, two O-methyltransferases involved in the biosynthesis of mycinamicin macrolide antibiotics.
Mg motors: We characterized the in vitro function of MycE and MycF, two O-methyltransferases involved in the biosynthesis of mycinamicin antibiotics. Each enzyme was confirmed to be an S-adenosyl-L-methionine (SAM)-dependent deoxysugar methyltransferase. Their optimal activities require the presence of Mg(2+). With the reconstituted in vitro assays, the order of mycinamicin VI-->III-->IV in the post-PKS (polyketide synthase) tailoring pathway of mycinamicin was unambiguously determined.
Life Sciences Institute, Department of Medicinal Chemistry, Chemistry, and Microbiology and Immunology, University of Michigan, 210 Washtenaw Avenue, Ann Arbor, MI 48109-2216, USA.
Shengying
S
Li
Yojiro
Y
Anzai
Kenji
K
Kinoshita
Fumio
F
Kato
David H
DH
Sherman
D000595:Amino Acid Sequence; D000900:Anti-Bacterial Agents / Q000096:biosynthesis* / Q000737:chemistry; D007700:Kinetics; D018942:Macrolides / Q000737:chemistry*; D008274:Magnesium / Q000378:metabolism; D008780:Methyltransferases / Q000378:metabolism; D008969:Molecular Sequence Data; D005810:Multigene Family; D010802:Phylogeny; D048630:Polyketide Synthases / Q000378:metabolism; D011497:Protein O-Methyltransferase / Q000737:chemistry* / Q000145:classification / Q000378:metabolism; D017386:Sequence Homology, Amino Acid
D000900:Anti-Bacterial Agents; D018942:Macrolides; C026686:mycinamicins; D048630:Polyketide Synthases; D008780:Methyltransferases; D011497:Protein O-Methyltransferase; C483301:S-adenosyl-L-methionine (R,S)-reticuline 7-O-methyltransferase; D008274:Magnesium