Locally administered T cells from mice immunized with lipopolysaccharide (LPS) accelerate LPS-induced bone resorption.
T cells play important roles in bone destruction and osteoclastogenesis and are found in chronic destructive bone lesions. Lipopolysaccharide (LPS) is one of several pathological factors involved in inflammatory bone destruction. We previously described the importance of T cells in the inflammatory bone resorption that occurs after repeated LPS administration. However, whether local or systemic T cells are important for inflammatory bone resorption and whether immunization of host animals influences bone resorption remain unclear. The present study examines the effects of local extant T cells from LPS-immunized mice on LPS-induced bone resorption. T cells from LPS-immunized or non-immunized mice were injected together with LPS into the gingival tissues of mice with severe combined immunodeficiency disease that lack both T and B cells. We histomorphometrically evaluated bone resorption at sites of T cell injections and examined the influence of T cells from LPS-immunized mice on osteoclastogenesis in vitro. We found that locally administered T cells from LPS-immunized but not non-immunized mice accelerated LPS-induced bone resorption in vivo. Moreover, T cells from LPS-immunized mice increased osteoclastogenesis in vitro induced by receptor activator of NF-kappa B ligand and LPS and anti-tumor necrosis factor (TNF)-alpha antibody inhibited this increase. These results demonstrated that local extant T cells accelerate inflammatory bone resorption. Furthermore, T cells from LPS-immunized mice appear to elevate LPS-induced bone resorption using TNF-alpha.
Department of Periodontology, Unit of Translational Medicine, Course of Medical and Dental Sciences, Nagasaki University Graduate School of Biomedical Sciences, 1-7-1 Sakamoto, Nagasaki 852-8588, Japan.
Yukio
Y
Ozaki
Takashi
T
Ukai
Masayuki
M
Yamaguchi
Miho
M
Yokoyama
Esperanza R Ayón
ER
Haro
Mayumi
M
Yoshimoto
Takashi
T
Kaneko
Miho
M
Yoshinaga
Hirotaka
H
Nakamura
Chiaki
C
Shiraishi
Yoshitaka
Y
Hara
D000818:Animals; D001862:Bone Resorption / Q000139:chemically induced* / Q000276:immunology*; D017252:CD3 Complex / Q000378:metabolism; D002478:Cells, Cultured; D005434:Flow Cytometry; D007150:Immunohistochemistry; D008070:Lipopolysaccharides / Q000276:immunology* / Q000494:pharmacology*; D008297:Male; D051379:Mice; D016513:Mice, SCID; D010010:Osteoclasts / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D053245:RANK Ligand / Q000378:metabolism; D013601:T-Lymphocytes / Q000276:immunology*; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology
D017252:CD3 Complex; D008070:Lipopolysaccharides; D053245:RANK Ligand; D014409:Tumor Necrosis Factor-alpha