Homogenous thionine-SiO2 nanocomposite spheres: sonochemical preparation, characterization, and application in H2O2 biosensor.
The electrochemical properties of new homogenous thionine (TH)-doped silica nanoparticles as a mediator were studied. The nanocomposites were synthesized by using sonochemical method. The sonication induced the strong combination between dye molecules and silica in the construction of the composites. By controlling reaction condition, face rougher uniform spheres could be achieved. The as-prepared nanocomposites can be used as the mediator to construct the biosensors with horseradish peroxidase coimmobilized in the gelatine matrix and cross-linked with formaldehyde. The described experimental evidences show the different ratio of ethanol to water can induce the changes in their diameters. As a consequence, the resulting biosensors exhibited amperometric response decreased with the increase of the spherical size. The resulting biosensor mediated by the 100 nm diameter of TH-doped silica nanoparticles exhibited very fast amperometric response, good stability and could be effectively used for the study of the electrocatalytic properties.
MOE Key Lab of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, 210093, P. R. China.
Hui
H
Yao
Jian-Min
JM
Hong
Na
N
Li
Shu
S
Xu
Jun-Jie
JJ
Zhu
D015374:Biosensing Techniques / Q000379:methods*; D004563:Electrochemistry; D004800:Enzymes, Immobilized / Q000737:chemistry / Q000378:metabolism; D006735:Horseradish Peroxidase / Q000737:chemistry / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000032:analysis* / Q000378:metabolism; D053761:Nanocomposites / Q000737:chemistry* / Q000648:ultrastructure; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D010316:Particle Size; D010640:Phenothiazines / Q000138:chemical synthesis* / Q000737:chemistry; D012680:Sensitivity and Specificity; D012822:Silicon Dioxide / Q000138:chemical synthesis* / Q000737:chemistry; D013010:Sonication
D004800:Enzymes, Immobilized; D010640:Phenothiazines; D012822:Silicon Dioxide; D006861:Hydrogen Peroxide; D006735:Horseradish Peroxidase; C009469:thionine