Ex vivo study of incorporation into adipocytes and lipolysis-inhibition effect of polycyclic aromatic hydrocarbons.
We have previously shown that benzo[a]pyrene (B[a]P) administrated at extremely low dose can cause weight gain in mice and that the increase in adipose tissue mass is due to inhibition of beta-adrenergic stimulation of lipolysis. Moreover we have suggested that in addition to its endocrine properties, adipose tissue act as a reservoir for many chemical carcinogens including Polycyclic Aromatic Hydrocarbons (PAHs). In this paper we show that B[a]P as well as the two C4 PAHs, pyrene and phenanthrene can bioaccumulate into adipocytes in a similar manner, but that at the difference of B[a]P, have no impact on epinephrine-induced lipolysis. On the basis of this ex vivo study, we therefore suggest that B[a]P may play a central role in carcinogenesis not only by inducing cancer through its mutagenic properties, but also by increasing the bioaccumulation capacity of the adipose tissue mass.
Cancer Research Center, Association for Research and Treatments Against Cancer (ARTAC), 57-59 rue de la convention, F-75015 Paris, France. philippei.artac@gmail.com
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Irigaray
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Lacomme
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Mejean
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Belpomme
D017667:Adipocytes / Q000187:drug effects* / Q000378:metabolism; D000818:Animals; D001564:Benzo(a)pyrene / Q000378:metabolism / Q000633:toxicity*; D004837:Epinephrine / Q000494:pharmacology; D005230:Fatty Acids, Nonesterified / Q000378:metabolism; D008066:Lipolysis / Q000187:drug effects*; D051379:Mice; D009153:Mutagens / Q000378:metabolism / Q000633:toxicity*; D010616:Phenanthrenes / Q000378:metabolism / Q000633:toxicity*; D011084:Polycyclic Aromatic Hydrocarbons; D011721:Pyrenes / Q000378:metabolism / Q000633:toxicity*
D005230:Fatty Acids, Nonesterified; D009153:Mutagens; D010616:Phenanthrenes; D011084:Polycyclic Aromatic Hydrocarbons; D011721:Pyrenes; D001564:Benzo(a)pyrene; C031181:phenanthrene; C030984:pyrene; D004837:Epinephrine