Laser microdissection of neonatal porcine endometrium for tissue-specific evaluation of relaxin receptor (RXFP1) expression in response to perinatal zearalenone exposure.
Porcine neonatal uterine relaxin receptor (RXFP1) expression is tissue compartment specific and estrogen sensitive. Here, procedures were established for laser microdissection, tissue capture, and quantification of the effects of perinatal exposure (14 days pre- to 21 days postnatal) to a selective estrogen receptor modulator of environmental origin, zearalenone (ZEA), on endometrial RXFP1 expression. Total RNA from captured endometrium was used to generate cDNA for quantitative reverse transcription-PCR. Cycle threshold values indicated that ZEA reduced (P < 0.06) endometrial RXFP1 expression on postnatal days 20-21.
Department of Animal Sciences, Auburn University, Auburn, Alabama 36849, USA.
Anne A
AA
Wiley
Johannes
J
Kauffold
Martin
M
Wähner
Bethany
B
Crean-Harris
Dori J
DJ
Miller
Carol A
CA
Bagnell
Frank F
FF
Bartol
D000818:Animals; D000831:Animals, Newborn; D004717:Endometrium / Q000187:drug effects / Q000378:metabolism*; D004968:Estrogens, Non-Steroidal / Q000494:pharmacology*; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects*; D007834:Lasers; D042282:Microdissection / Q000379:methods*; D011247:Pregnancy; D043562:Receptors, G-Protein-Coupled / Q000235:genetics / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013552:Swine; D015025:Zearalenone / Q000494:pharmacology*
D004968:Estrogens, Non-Steroidal; D043562:Receptors, G-Protein-Coupled; D015025:Zearalenone