Effects of acute alcohol consumption and vitamin E co-treatment on oxidative stress parameters in rats tongue.
The aim of this study was to evaluate the effects of acute alcohol consumption and vitamin E co-treatment upon oxidative stress parameters in rats tongue. Thirty-eight, Wistar rats were separated into five groups (alcohol, alcohol/vitamin E, control, Tween, vitamin E). Alcohol and alcohol vitamin E groups had the standard diet, and 40% alcohol on drinking water. Other groups were fed with the same standard diet and water ad libitum. Vitamin E was given by gavage to vitamin E and alcohol/vitamin E rats twice a week. Alcohol and control groups were subjected to saline gavage and Tween group to 5% Tween 80 solution, the vitamin E vehicle. At day 14, the animals were anesthetized and specimens were obtained from tongue. Lipid peroxidation (TBARS), protein oxidative damage, catalase (CAT) and superoxide dismutase (SOD) activities were quantified. Alcohol group decreased TBARS in relation to control group and alcohol vitamin-treated animals decreased TBARS when compared to Tween and vitamin E groups. SOD activity was lower and CAT activity was higher in animals treated with both alcohol and vitamin E. These results suggest that short-term alcohol consumption decreases lipid peroxidation levels. Alternatively, alcohol/vitamin E group increased CAT, showing the toxicity of this association.
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Laboratório de Histopatologia Prof. Dr. J.J. Barbachan (Patologia Bucal), Faculdade de Odontologia, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, Brazil. vcarrard@yahoo.com.br
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Mendez
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Moreira
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Sant'Ana Filho
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D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001835:Body Weight / Q000187:drug effects; D002374:Catalase / Q000378:metabolism; D002492:Central Nervous System Depressants / Q000494:pharmacology*; D000431:Ethanol / Q000494:pharmacology*; D005260:Female; D015227:Lipid Peroxidation / Q000187:drug effects; D009747:Nutritional Physiological Phenomena; D009929:Organ Size / Q000187:drug effects; D018384:Oxidative Stress / Q000187:drug effects*; D011136:Polysorbates / Q000494:pharmacology; D050050:Protein Carbonylation / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar; D013482:Superoxide Dismutase / Q000378:metabolism; D013501:Surface-Active Agents / Q000494:pharmacology; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism; D014059:Tongue / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D014810:Vitamin E / Q000494:pharmacology*
D000975:Antioxidants; D002492:Central Nervous System Depressants; D011136:Polysorbates; D013501:Surface-Active Agents; D017392:Thiobarbituric Acid Reactive Substances; D014810:Vitamin E; D000431:Ethanol; D002374:Catalase; D013482:Superoxide Dismutase