Cloning and characterization of a sucrose synthase-encoding gene from muskmelon.
A full-length cDNA clone encoding sucrose synthase (SS; EC 2.4.1.13) was isolated from muskmelon (Cucumis melo L.) by RT-PCR and RACE. The clone, designated as CmSS1, contains 2,585 nucleotides with an open reading frame of 2,412 nucleotides. The deduced 804 amino acid sequence showed high identities with other plant sucrose synthase. Real time PCR analysis indicated that CmSS1 expression differed among root, stem, leaf, flower and fruit tissues. The analysis during fruit development indicated that CmSS1 mRNA showed its maximum level at 5 days after pollination (DAP) and decreased gradually during fruit development until its minimum level in mature fruit. The sucrose content was very low in fruit before 20 DAP but increased dramatically between 20 and 30 DAP during fruit development. However, SS activities in both direction of sucrose synthesis and sucrose cleavage were very low and changed little during fruit development, suggesting that SS may play little role in determining sucrose accumulation during muskmelon fruit.
State Key Laboratory of Crop Biology, College of Horticulture Science and Engineering, Shandong Agricultural University, 271018, Tai'an, China.
Xiaoxia
X
Wen
Wenqian
W
Zhang
Yanqing
Y
Feng
Xiyan
X
Yu
D000595:Amino Acid Sequence; D003001:Cloning, Molecular; D028463:Cucumis melo / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D005638:Fruit / Q000235:genetics / Q000378:metabolism / Q000502:physiology; D018507:Gene Expression Regulation, Developmental; D015971:Gene Expression Regulation, Enzymologic; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D005964:Glucosyltransferases / Q000235:genetics* / Q000302:isolation & purification; D008969:Molecular Sequence Data; D017386:Sequence Homology, Amino Acid; D013395:Sucrose / Q000378:metabolism
D013395:Sucrose; D005964:Glucosyltransferases; C022699:sucrose synthase