Effect of cerebellar reversible inactivations on the acquisition of trace conditioned eyeblink responses in guinea pigs: comparison of short and long trace intervals.
The cerebellum has been proved to be essential for the acquisition of delay eyeblink conditioning, but its contribution to the acquisition of trace eyeblink conditioning (TEBC) has not been fully determined. In the present study, using chemically reversible inactivation techniques, we examined the relative contribution of ipsilateral cerebellum to the acquisition of TEBC using different time length of trace interval (TI) in guinea pigs. It was found that inactivations of the left intermediate cerebellum with a GABA(A) receptor agonist muscimol during training completely prevented the acquisition of TEBC using a relatively short (50 ms) TI, instead of the acquisition of TEBC using a relatively long (250 ms) TI. However, inactivations of the left intermediate cerebellum totally abolished the well-established left trace conditioned eyeblink responses (CRs) regardless of the time length of TI. These results suggested that while the ipsilateral cerebellum is essential for the expression of trace CRs, its contribution to the acquisition of trace CRs appears to mainly depend on the time length of TI.
Hu
Bo
B
Department of Neurosurgery, Southwest Hospital, Third Military Medical University, Chongqing 400038, China.
Yang
Li
L
Huang
Lü-Shuai
LS
Chen
Hao
H
Zeng
Yuan
Y
Feng
Hua
H
Sui
Jian-Feng
JF
D000704:Analysis of Variance; D000818:Animals; D001245:Association Learning / Q000187:drug effects / Q000502:physiology; D002404:Catheterization; D002531:Cerebellum / Q000187:drug effects / Q000502:physiology*; D003215:Conditioning, Eyelid / Q000502:physiology*; D007839:Functional Laterality; D018755:GABA Agonists / Q000008:administration & dosage; D006168:Guinea Pigs; D008845:Microinjections; D009118:Muscimol / Q000008:administration & dosage; D013997:Time Factors
D018755:GABA Agonists; D009118:Muscimol