[Expression and significance of new candidate tumor suppressor gene N-Myc downstream-regulated gene 2 in colorectal cancer].
OBJECTIVE
To determine the expression of new candidate tumor suppressor gene N-Myc downstream-regulated gene 2(Ndrg2) in colorectal cancer with different differentiation, and analyze its clinical significance.


METHODS
Specimens of 50 colorectal cancer patients with different differentiation were collected. Immunohistochemistry and Western blot were used to examine the expression of Ndrg2. Colorectal cancer tissue array in large scale was applied to analyze the expression of Ndrg2 and the statistics analysis was performed referring to the patients information of the array.


RESULTS
Among 50 cases, Ndrg2 expression level of colorectal cancer was significantly lower in 32 cases as compared to adjacent and normal tissue of the same individual, while Ndrg2 expression of adjacent tissue was significantly lower than that of normal tissue. Ndrg2 protein levels increased from poor-differentiated to well-differentiated carcinomas(P=0.005).


CONCLUSIONS
The expression of Ndrg2 in different differentiated colorectal cancer tissues show a significant distinction. Ndrg2 may be involved in the regulation of differentiation in colorectal cancer.
Department of Gastrointestinal Surgery, Xijing Hospital, The Fourth Military Medical University, Xi'an 710033, China.
Hai
H
Shi
Wei-Zhong
WZ
Wang
Li-Bo
LB
Yao
Jian
J
Zhang
Qin
Q
Yin
Chun-Sheng
CS
Xu
Da-Ke
DK
Chu
Guang-Long
GL
Dong
Hong-Wei
HW
Zhang
Ji-Peng
JP
Li
D000328:Adult; D000368:Aged; D015179:Colorectal Neoplasms / Q000378:metabolism* / Q000473:pathology*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D040081:Protein Array Analysis; D025521:Tumor Suppressor Proteins / Q000378:metabolism*; D055815:Young Adult
C466228:NDRG2 protein, human; D025521:Tumor Suppressor Proteins