The fluorescence enhancement of quercetin-nucleic acid system and the analytical application.
Nucleic acid can greatly enhance the fluorescence intensity of quercetin in HMTA-HCl (pH 5.5) buffer. The enhanced intensity is in proportion to the concentration of nucleic acids in the range 5.0 x 10(-9) to 1.0 x 10(-6) g/mL for fsDNA, 5.0 x 10(-9) to 7.0 x 10(-7) g/mL for ctDNA and 5.0 x 10(-9) to 1.0 x 10(-6) g/mL for yRNA, and their detection limits (S/N = 3) are 3.5 x 10(-9), 7.8 x 10(-10) and 2.6 x 10(-9) g/mL, respectively. In comparison with most reported fluorescent probes for the determination of nucleic acids, the proposed probe has higher sensitivity and lower toxicity. The interaction investigation indicates that quercetin binds with double-strand DNA in groove binding mode, resulting in fluorescence enhancement of this system.
Liu
Yuan
Y
Key Laboratory of Colloid and Interface Chemistry (Shandong University), Ministry of Education, School of Chemistry and Chemical Engineering, Shandong University, Jinan 250100, People's Republic of China.
Wu
Xia
X
Zhou
Haiping
H
Liu
Xiaoyu
X
Zhang
Fengju
F
Yang
Jinghe
J
D005456:Fluorescent Dyes / Q000737:chemistry*; D057230:Limit of Detection; D015394:Molecular Structure; D009696:Nucleic Acids / Q000032:analysis* / Q000737:chemistry; D011794:Quercetin / Q000737:chemistry*; D013050:Spectrometry, Fluorescence
D005456:Fluorescent Dyes; D009696:Nucleic Acids; D011794:Quercetin