Alteration of mitochondrial DNA sequence and copy number in nasal polyp tissue.
This study was designed to investigate the possibility that mtDNA mutations might arise in inflammatory or chronically damaged nasal polyp tissue from 23 patients. Thirteen patients (57%) displayed nasal polyp tissue-specific mtDNA mutations in the hypervariable segment of the control region and cytochrome b gene, which were not found in the corresponding blood cells and/or adjacent normal tissue. Nasal polyp tissue-specific length heteroplasmic mutations were also detected in nucleotide position (np) 303-315 homopolymeric poly C track (39%), np 514-523 CA repeats (17%) and np 16184-16193 poly C track (30%). The average mtDNA copy number was about three times higher in nasal polyp tissue than in the corresponding peripheral blood cells and adjacent non-polyp tissues. The level of reactive oxygen species (ROS) was significantly higher in the nasal polyp tissues compared to those from the corresponding samples. High level of ROS in nasal polyp tissue may contribute to development of mtDNA mutations, which may play a crucial role in the vicious cycle of pathophysiology of nasal polyps.
Department of Laboratory Medicine and Molecular Genetics, Chonnam National University Medical School and Chonnam National University Hwasun Hospital, 160 Ilsimri, Hwasun-eup, Hwasun-gun, Jeollanam-do 519-809, South Korea.
Sang-Young
SY
Park
Myung-Geun
MG
Shin
Hye-Ran
HR
Kim
Ji-Yeon
JY
Oh
Soo-Hyun
SH
Kim
Jong-Hee
JH
Shin
Yong-Bum
YB
Cho
Soon-Pal
SP
Suh
Dong-Wook
DW
Ryang
D000293:Adolescent; D000328:Adult; D000368:Aged; D045303:Cytochromes b / Q000235:genetics*; D004272:DNA, Mitochondrial / Q000235:genetics*; D005260:Female; D018628:Gene Dosage; D006651:Histocytochemistry; D006801:Humans; D008297:Male; D008875:Middle Aged; D009154:Mutation; D009298:Nasal Polyps / Q000737:chemistry / Q000235:genetics / Q000473:pathology*; D011247:Pregnancy; D017382:Reactive Oxygen Species / Q000032:analysis; D017422:Sequence Analysis, DNA
D004272:DNA, Mitochondrial; D017382:Reactive Oxygen Species; D045303:Cytochromes b