Dissection of barley chromosome 3H in common wheat and a comparison of 3H physical and genetic maps.
We used the gametocidal system to dissect a barley chromosome 3H added to common wheat. The gametocidal system induced chromosomal structural changes in the 3H addition line of common wheat, and we cytologically screened for rearranged chromosomes involving the 3H chromosome by in situ hybridization (FISH/GISH). We established 50 common wheat lines carrying single rearranged (or dissected) 3H chromosomes of independent origin. The dissected 3H chromosomes were either deletions or translocations with wheat chromosomes, and their breakpoints were in the centromere/the long arm/the short arm in a rough ratio of 1:2:2. We used these so-called 3H dissection lines to map 36 EST markers that were polymorphic between euploid common wheat and the 3H addition line and that had been used for the construction of a 3H genetic map. We conducted PCR analysis to detect the EST markers in the dissection lines. The results of the PCR analysis, which mostly corresponded to the retained or lost segments of the dissected 3H chromosomes, allowed us to place the 36 EST markers into 20 chromosomal regions flanked by the breakpoints of the dissected chromosomes. We compared this physical map constructed in this study with a 3H genetic map constructed using the same EST markers. The order of all EST markers was consistent between the two maps. We briefly discuss on the advantage of the physical mapping using dissection lines over genetic mapping.
Sakai
Kazuhiko
K
Laboratory of Plant Genetics, Graduate School of Agriculture, Kyoto University, Kyoto, Japan.
Nasuda
Shuhei
S
Sato
Kazuhiro
K
Endo
Takashi R
TR
D032461:Chromosomes, Plant / Q000235:genetics*; D001467:Hordeum / Q000235:genetics*; D020161:Physical Chromosome Mapping / Q000379:methods*; D014908:Triticum / Q000235:genetics