An innovative method to measure skin pigmentation.
OBJECTIVE
It is crucial to establish an accurate method for measuring skin pigmentation in cosmetic science and clinical dermatology. Here, we report a non-invasive precise method for measuring skin melanin content.


METHODS
In order to determine the concentrations of melanin and hemoglobin in skin, we used the spectrum resolution (SR) method. In brief, the absorption spectrum of the skin was calculated from the reflection spectrum using a spectrophotometer. The concentrations of melanin and hemoglobin were then determined using a multiple regression analysis, assuming that the absorption spectrum of the skin is expressed as a linear summation of the absorptions of melanin and hemoglobin according to the Lambert-Beer law. The skin changes in the volar forearm, which had been irradiated by ultraviolet rays (UV), were observed daily by the SR method.


RESULTS
A multiple regression analysis with an absorption spectrum of 500-700 nm was performed. The multiple correlation coefficient was 0.993, resulting in a satisfactory precise estimate of the concentrations of melanin. After UV irradiation, the concentration of melanin monitored by the SR method increased until 8 days and decreased gradually afterwards.


CONCLUSIONS
The SR method allows the evaluation of the changes of epidermal melanin induced by UV irradiation.
Shiseido Research Center, Yokohama, Japan. yuji.masuda@to.shiseido.co.jp
Yuji
Y
Masuda
Toyonobu
T
Yamashita
Tetsuji
T
Hirao
Motoji
M
Takahashi
D000465:Algorithms; D006454:Hemoglobins / Q000032:analysis*; D006801:Humans; D008543:Melanins / Q000032:analysis*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012867:Skin / Q000737:chemistry*; D012880:Skin Pigmentation / Q000502:physiology*; D013057:Spectrum Analysis / Q000379:methods*
D006454:Hemoglobins; D008543:Melanins