[Application of liquid chromatography coupled with mass spectrometry (LC/MS) to determine antidepressants in blood samples].
A simultaneous determination of twelve antidepressant drugs (amitriptyline, chlorpromazine, clomipramine, doxepin, fluoxetine, levomepromazine, maprotiline, paroxetine, perazine, promazine, sertraline, thioridazine) in blood samples was developed using high-performance liquid chromatography with mass spectrometry (LC/MS). Blood samples were extracted with acetonitrile and separated by HPLC on a reversed phase C18 column with a mobile phase consisting of acetonitrile -0.1% trifluoroacetic acid (50:50 (v/v) at a flow-rate of 0.4 ml/min. The analytes were determined using electrospray ionization in a single quadrupole mass spectrometer. LC-ESI-MS was performed in the selected-ion monitoring (SIM) mode using target ions. The validated method was successfully used to analyze antidepressants in blood samples collected from four victims of suicide by hanging.
Pufal
Ewa
E
Katedry Medycyny Sadowej UMK w Toruniu, Collegium Medicum im. Ludwika Rydygiera w Bydgoszczy.
Sykutera
Marzena
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D000929:Antidepressive Agents, Tricyclic / Q000097:blood*; D001774:Blood Chemical Analysis; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005554:Forensic Medicine / Q000379:methods*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006801:Humans; D007202:Indicators and Reagents / Q000737:chemistry; D011044:Poland; D012680:Sensitivity and Specificity; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
D000929:Antidepressive Agents, Tricyclic; D007202:Indicators and Reagents