Downregulation of telomerase activity in human promyelocytic cell line using RNA interference.
Telomerase is a ribonucleoprotein complex. It consists of two main components, human telomerase reverse transcriptase (hTERT) and human telomerase RNA. High telomerase activity is present in most malignant cells, but it is barely detectable in majority of somatic cells. The direct correlation between telomerase reactivation and carcinogens has made hTERT a key target for anticancer therapeutic studies. In this study, for the first time, we evaluated the ability of the new generation of short interfering RNA (siRNA) to regulate telomerase activity in the human promyelocytic leukemia cell line (HL-60). Transient transfection cell line by hTERT siRNAs resulted in statistically significant suppression of hTERT messenger RNAs which were detected by quantitative real-time polymerase chain reaction, while the expressed hTERT protein levels were measured by flow cytometry. The results of telomeric repeat amplification protocol showed that telomerase activity was significantly reduced upon transfection of the HL-60 cell line with hTERT siRNAs. The results of this study showed that telomerase activity and cell proliferation were efficiently inhibited in the hTERT siRNA-treated leukemic cell line.
National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran.
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Miri-Moghaddam
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A
Deezagi
Z S
ZS
Soheili
D049109:Cell Proliferation; D015536:Down-Regulation; D018922:HL-60 Cells / Q000378:metabolism*; D006801:Humans; D012313:RNA / Q000235:genetics / Q000378:metabolism; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics / Q000378:metabolism; D019098:Telomerase / Q000235:genetics / Q000378:metabolism; D014162:Transfection
D034741:RNA, Small Interfering; C422952:telomerase RNA; D012313:RNA; D019098:Telomerase