Encapsulation of alpha-amylase into starch-based biomaterials: an enzymatic approach to tailor their degradation rate.
This paper reports the effect of alpha-amylase encapsulation on the degradation rate of a starch-based biomaterial. The encapsulation method consisted in mixing a thermostable alpha-amylase with a blend of corn starch and polycaprolactone (SPCL), which were processed by compression moulding to produce circular disks. The presence of water was avoided to keep the water activity low and consequently to minimize the enzyme activity during the encapsulation process. No degradation of the starch matrix occurred during processing and storage (the encapsulated enzyme remained inactive due to the absence of water), since no significant amount of reducing sugars was detected in solution. After the encapsulation process, the released enzyme activity from the SPCL disks after 28days was found to be 40% comparatively to the free enzyme (unprocessed). Degradation studies on SPCL disks, with alpha-amylase encapsulated or free in solution, showed no significant differences on the degradation behaviour between both conditions. This indicates that alpha-amylase enzyme was successfully encapsulated with almost full retention of its enzymatic activity and the encapsulation of alpha-amylase clearly accelerates the degradation rate of the SPCL disks, when compared with the enzyme-free disks. The results obtained in this work show that degradation kinetics of the starch polymer can be controlled by the amount of encapsulated alpha-amylase into the matrix.
3B's Research Group - Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, AvePark, 4806-909 Taipas, Guimarães, Portugal. hazevedo@dep.uminho.pt
Helena S
HS
Azevedo
Rui L
RL
Reis
D000042:Absorption; D001672:Biocompatible Materials / Q000737:chemistry*; D003460:Crystallization / Q000379:methods; D003692:Delayed-Action Preparations / Q000737:chemistry*; D004058:Diffusion; D004339:Drug Compounding / Q000379:methods; D004800:Enzymes, Immobilized / Q000737:chemistry; D008422:Materials Testing; D016062:Porosity; D013213:Starch / Q000737:chemistry*; D013499:Surface Properties; D014867:Water / Q000737:chemistry*; D000516:alpha-Amylases / Q000737:chemistry*
D001672:Biocompatible Materials; D003692:Delayed-Action Preparations; D004800:Enzymes, Immobilized; D014867:Water; D013213:Starch; D000516:alpha-Amylases