Antihyperglycemic, antioxidant and antiglycation activities of mulberry leaf extract in streptozotocin-induced chronic diabetic rats.
In Thailand, beverages containing mulberry leaf (Morus alba L.) are believed to promote good health, especially in people with diabetes. We examined the effects of long-term administration of an ethanolic extract of mulberry leaf (MA) on blood glucose, oxidative damage, and glycation in streptozotocin-induced diabetic rats. Daily administration of 1 g/kg MA for six weeks decreased blood glucose by 22%, which was comparable to the effect of 4 U/kg insulin. Lipid peroxidation, measured as malondialdehyde and lipid hydroperoxide concentrations (3.50 +/- 0.33 and 3.76 +/- 0.18 muM, respectively) decreased significantly (P < 0.05) compared to nontreated control diabetic rats (8.19 +/- 0.45 and 7.50 +/- 0.46 muM, respectively). Hemoglobin A(1C), a biomarker for chronic exposure to high concentration of glucose, was also significantly decreased in the MA-treated group (6.78 +/- 0.30%) in comparison to untreated group (9.02 +/- 0.30%). The IC(50) of in vitro antiglycation and free radical scavenging activities of MA were 16.4 +/- 5.6 microg/ml and 61.7 +/- 2.1 microg/ml, respectively. These findings support that long-term administration of MA has antihyperglycemic, antioxidant and antiglycation effects in chronic diabetic rats, which may be beneficial as food supplement for diabetics.
Naowaboot
Jarinyaporn
J
Department of Pharmacology, Khon Kaen University, Khon Kaen 40002, Thailand.
Pannangpetch
Patchareewan
P
Kukongviriyapan
Veerapol
V
Kongyingyoes
Bunkerd
B
Kukongviriyapan
Upa
U
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology / Q000627:therapeutic use*; D001786:Blood Glucose / Q000378:metabolism; D003921:Diabetes Mellitus, Experimental / Q000097:blood / Q000188:drug therapy*; D006442:Glycated Hemoglobin / Q000378:metabolism; D007004:Hypoglycemic Agents / Q000494:pharmacology / Q000627:therapeutic use*; D008054:Lipid Peroxides / Q000097:blood; D008297:Male; D008315:Malondialdehyde / Q000097:blood; D031625:Morus; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology / Q000627:therapeutic use*; D018515:Plant Leaves; D051381:Rats; D017207:Rats, Sprague-Dawley
D000975:Antioxidants; D001786:Blood Glucose; D006442:Glycated Hemoglobin; D007004:Hypoglycemic Agents; D008054:Lipid Peroxides; D008315:Malondialdehyde; D010936:Plant Extracts