Barley-derived beta-D-glucan induces immunostimulation via a dectin-1-mediated pathway.
Barley-derived beta-glucan, a linear mixed-linkage beta-glucan composed of 1,3- and 1,4-beta-D-glucopyranose polymers, binds to dectin-1. However, whether it can trigger signal transduction via dectin-1 remains unclear. In this study, we used a reporter gene assay to determine whether barley-derived beta-d-glucan can activate NF-kappaB via dectin-1-mediated signaling when dectin-1 is cotransfected with Syk, CARD9, and Bcl10 in 293T cells. We found that barley-derived beta-D-glucan can activate NF-kappaB leading to cytokine production when dectin-1, Syk, CARD9, and Bcl10 are coexpressed in the cells. We also found that barley-derived beta-D-glucan can induce the phosphorylation of Syk and production of IL-6 in thioglycolate-elicited peritoneal macrophages. These results indicated that the immunostimulatory effects of barley-derived beta-d-glucan might be exerted, at least in part, via dectin-1.
Laboratory for Immunopharmacology of Microbial Products, School of Pharmacy, Tokyo University of Pharmacy and Life Sciences, 1432-1, Horinouchi, Hachioji, Tokyo, Japan.
Rui
R
Tada
Futoshi
F
Ikeda
Kosuke
K
Aoki
Masashi
M
Yoshikawa
Yuya
Y
Kato
Yoshiyuki
Y
Adachi
Asuka
A
Tanioka
Ken-ichi
K
Ishibashi
Kazufumi
K
Tsubaki
Naohito
N
Ohno
D048868:Adaptor Proteins, Signal Transducing / Q000276:immunology / Q000378:metabolism; D000818:Animals; D000074018:B-Cell CLL-Lymphoma 10 Protein; D053468:CARD Signaling Adaptor Proteins; D002240:Carbohydrate Sequence; D002460:Cell Line; D001467:Hordeum / Q000737:chemistry / Q000276:immunology*; D015850:Interleukin-6 / Q000276:immunology / Q000378:metabolism; D047908:Intracellular Signaling Peptides and Proteins / Q000276:immunology / Q000378:metabolism; D037181:Lectins, C-Type; D008070:Lipopolysaccharides / Q000494:pharmacology; D008297:Male; D008565:Membrane Proteins / Q000187:drug effects / Q000276:immunology / Q000378:metabolism*; D051379:Mice; D008811:Mice, Inbred DBA; D008969:Molecular Sequence Data; D016328:NF-kappa B / Q000276:immunology / Q000378:metabolism*; D009419:Nerve Tissue Proteins / Q000187:drug effects / Q000276:immunology / Q000378:metabolism*; D010766:Phosphorylation / Q000187:drug effects / Q000276:immunology; D011505:Protein-Tyrosine Kinases / Q000276:immunology / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects* / Q000276:immunology; D000072377:Syk Kinase; D014162:Transfection; D015054:Zymosan / Q000276:immunology / Q000494:pharmacology; D047071:beta-Glucans / Q000737:chemistry / Q000276:immunology*; D000099181:Dectin-1
D048868:Adaptor Proteins, Signal Transducing; D000074018:B-Cell CLL-Lymphoma 10 Protein; D053468:CARD Signaling Adaptor Proteins; D015850:Interleukin-6; D047908:Intracellular Signaling Peptides and Proteins; D037181:Lectins, C-Type; D008070:Lipopolysaccharides; D008565:Membrane Proteins; D016328:NF-kappa B; D009419:Nerve Tissue Proteins; D011505:Protein-Tyrosine Kinases; D000072377:Syk Kinase; D015054:Zymosan; D047071:beta-Glucans; D000099181:Dectin-1; C488088:Bcl10 protein, mouse; C513280:Card9 protein, mouse; C000606897:Syk protein, mouse