NMR-based metabonomic analysis on effect of light on production of antioxidant phenolic compounds in submerged cultures of Inonotus obliquus.
This study was designed to investigate the light effect on biosynthesis of antioxidant phenolic compounds by Inonotus obliquus grown in submerged cultures using (1)H NMR spectroscopy combining multivariate pattern recognition strategies. I. obliquus were exposed to a range of light conditions and resultant data were compared to those from field-grown sclerotia and the mycelia grown in daylight. Daylight illumination inhibited biosynthesis of davallialactone and phelligridins and other hispidin analogs. Continuous darkness enhanced the formation of phelligridins, davallialactone and inoscavins. Phelligridins and davallialactone also occurred in the mycelia grown in blue and red light with levels lower than those found in darkness. In addition, polyphenols synthesized under daylight conditions showed less potential antioxidant activity than those determined with other light regimes. These findings demonstrate that light regulates biosynthesis of polyphenols in I. obliquus and their subsequent antioxidant activities, and (1)H NMR-based metabolic profiling is a cost-effective approach for evaluating light effects on fungal metabolisms.
Key Laboratory for Biotechnology on Medicinal Plants of Jiangsu Province, Xuzhou Normal University, Xuzhou, China. yyzw@xznu.edu.cn
Weifa
W
Zheng
Meimei
M
Zhang
Yanxia
Y
Zhao
Kangjie
K
Miao
Hong
H
Jiang
D000975:Antioxidants / Q000378:metabolism*; D001487:Basidiomycota / Q000187:drug effects / Q000254:growth & development* / Q000528:radiation effects*; D018533:Biomass; D016002:Discriminant Analysis; D000431:Ethanol; D016166:Free Radical Scavengers / Q000494:pharmacology; D016018:Least-Squares Analysis; D008027:Light; D009682:Magnetic Resonance Spectroscopy; D055432:Metabolomics; D025282:Mycelium / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects; D010636:Phenols / Q000378:metabolism*
D000975:Antioxidants; D016166:Free Radical Scavengers; D010636:Phenols; D000431:Ethanol