Enzymatic detection of As(III) in aqueous solution using alginate immobilized pumpkin urease: optimization of process variables by response surface methodology.
Urease immobilized on alginate was utilized to detect and quantify As(3+) in aqueous solution. Urease from the seeds of pumpkin (vegetable waste) was purified to apparent homogeneity by heat treatment and gel filtration (Sephadex G-200). Further enzyme was entrapped in 3.5% alginate beads. Urea hydrolysis by enzyme revealed a clear dependence on the concentration and interaction time of As(3+). The process variables effecting the quantitation of As(3+) was investigated using central composite design with Minitab 15 software. The predicted results were found in good agreement (R(2)=96.71%) with experimental results indicating the applicability of proposed model. The multiple regression analysis and ANOVA showed that enzyme activity decreased with increase of As(3+) concentration and interaction time. 3D plot and contour plot between As(3+) concentration and interaction time was helpful to predict residual activity of enzyme for a particular As(3+) at a particular time.
Department of Biochemistry, Faculty of Science, Banaras Hindu University, Varanasi, Uttar Pradesh, India. mahetalat04@gmail.com
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D000464:Alginates / Q000737:chemistry*; D000704:Analysis of Variance; D001151:Arsenic / Q000032:analysis*; D028464:Cucurbita / Q000201:enzymology*; D052918:Environmental Restoration and Remediation / Q000379:methods*; D004800:Enzymes, Immobilized / Q000378:metabolism*; D020723:Glucuronic Acid / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D007477:Ions; D012044:Regression Analysis; D012995:Solubility; D012996:Solutions; D013379:Substrate Specificity; D013499:Surface Properties; D013997:Time Factors; D014510:Urease / Q000378:metabolism*; D014867:Water / Q000737:chemistry*
D000464:Alginates; D004800:Enzymes, Immobilized; D006603:Hexuronic Acids; D007477:Ions; D012996:Solutions; D014867:Water; D020723:Glucuronic Acid; D014510:Urease; D001151:Arsenic