INSL3 plays a role in the balance between bone formation and resorption.
We have recently demonstrated that the disruption of the INSL3 hormonal system, as observed in humans with mutations in the RXFP2 gene and in Rxfp2-deficient mice, might affect the equilibrium of the osteoblast-osteoclast system, resulting in an imbalance between bone formation and bone resorption that may result in reduced bone mass. In the present study we have better characterized the in vitro effects of INSL3 on human osteoblasts. Stimulation of human primary osteoblasts with INSL3 at serial concentrations (1 pM, 1 nM, and 1 microM) induced a dose-dependent increase in osteoblast proliferation and expression of specific osteoblast genes.
Pepe
Anastasia
A
Department of Histology, Section of Clinical Pathology & Centre for Male Gamete Cryopreservation, University of Padova, Padova, Italy.
Ferlin
Alberto
A
Gianesello
Lisa
L
Facciolli
Arianna
A
Agoulnik
Alexander I
AI
Foresta
Carlo
C
D001862:Bone Resorption / Q000378:metabolism*; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D024042:Collagen Type I / Q000235:genetics; D000090602:Collagen Type I, alpha 1 Chain; D024142:Collagen Type VI / Q000235:genetics; D004305:Dose-Response Relationship, Drug; D005786:Gene Expression Regulation / Q000187:drug effects / Q000235:genetics; D006801:Humans; D007328:Insulin / Q000378:metabolism* / Q000494:pharmacology; D016328:NF-kappa B / Q000235:genetics; D050778:NFATC Transcription Factors / Q000235:genetics; D010006:Osteoblasts / Q000187:drug effects / Q000378:metabolism; D010012:Osteogenesis / Q000502:physiology*; D015676:Osteonectin / Q000235:genetics; D011506:Proteins / Q000378:metabolism* / Q000494:pharmacology; D020133:Reverse Transcriptase Polymerase Chain Reaction
C504344:COL6A2 protein, human; D024042:Collagen Type I; D000090602:Collagen Type I, alpha 1 Chain; D024142:Collagen Type VI; D007328:Insulin; C084797:Leydig insulin-like protein; D016328:NF-kappa B; D050778:NFATC Transcription Factors; C494003:NFATC1 protein, human; D015676:Osteonectin; D011506:Proteins