Effect of dynamic high pressure homogenization on the aggregation state of soy protein.
Although soy proteins are often employed as functional ingredients in oil-water emulsions, very little is known about the aggregation state of the proteins in solution and whether any changes occur to soy protein dispersions during homogenization. The effect of dynamic high pressure homogenization on the aggregation state of the proteins was investigated using microdifferential scanning calorimetry and high performance size exclusion chromatography coupled with multiangle laser light scattering. Soy protein isolates as well as glycinin and beta-conglycinin fractions were prepared from defatted soy flakes and redispersed in 50 mM sodium phosphate buffer at pH 7.4. The dispersions were then subjected to homogenization at two different pressures, 26 and 65 MPa. The results demonstrated that dynamic high pressure homogenization causes changes in the supramolecular structure of the soy proteins. Both beta-conglycinin and glycinin samples had an increased temperature of denaturation after homogenization. The chromatographic elution profile showed a reduction in the aggregate concentration with homogenization pressure for beta-conglycinin and an increase in the size of the soluble aggregates for glycinin and soy protein isolate.
Department of Food Science, University of Guelph, Guelph, Ontario N1G 2W1, Canada.
Maneephan
M
Keerati-U-Rai
Milena
M
Corredig
D052179:Antigens, Plant / Q000737:chemistry / Q000302:isolation & purification; D002152:Calorimetry, Differential Scanning; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D005511:Food Handling / Q000379:methods*; D005916:Globulins / Q000737:chemistry / Q000302:isolation & purification; D008970:Molecular Weight; D011312:Pressure; D011489:Protein Denaturation; D055314:Seed Storage Proteins / Q000737:chemistry / Q000302:isolation & purification; D030262:Soybean Proteins / Q000737:chemistry* / Q000302:isolation & purification
D052179:Antigens, Plant; D005916:Globulins; D055314:Seed Storage Proteins; D030262:Soybean Proteins; C013894:beta-conglycinin protein, Glycine max; C011474:glycinin