Embryonic stem cell-derived cardiomyocytes as a model to study fetal arrhythmia related to maternal disease.
Embryonic stem cell-derived cardiomyocytes (ESC-CM) have many of the phenotypic properties of authentic cardiomyocytes, and great interest has been shown in their possibilities for modelling human disease. Obstetric cholestasis affects 1 in 200 pregnant women in the United Kingdom. It is characterized by raised serum bile acids and complicated by premature delivery and unexplained fetal death at late gestation. It has been suggested that the fetal death is caused by the enhanced arrhythmogenic effect of bile acids in the fetal heart, and shown that neonatal susceptibility to bile acid-induced arrhythmia is lost in the adult rat cardiomyocyte. However, the mechanisms of the observed bile acid effects are not fully understood and their in vivo study in human beings is difficult. Here we use ESC-CM from both human and mouse ESCs to test our proposal that immature cardiomyocytes are more susceptible to the effect of raised bile acids than mature ones. We show that early ESC-CM exhibit bile acid-induced disruption of rhythm, depression of contraction and desynchronization of cell coupling. In both species the ESC-CM become resistant to these arrhythmias as the cells mature, and this develops in line with the respective gestational periods of mouse and human. This represents the first demonstration of the use of ESC-CM as a model system for human cardiac pathology, and opens the way for both investigation of mechanisms and a high throughput screen for drug discovery.
National Heart and Lung Institute, Imperial College London, London, UK.
Siti H Sheikh
SH
Abdul Kadir
Nadire N
NN
Ali
Maxime
M
Mioulane
Marta
M
Brito-Martins
Shadi
S
Abu-Hayyeh
Gabor
G
Foldes
Alexey V
AV
Moshkov
Catherine
C
Williamson
Sian E
SE
Harding
Julia
J
Gorelik
D000818:Animals; D001145:Arrhythmias, Cardiac / Q000473:pathology*; D001647:Bile Acids and Salts / Q000378:metabolism*; D002118:Calcium / Q000378:metabolism; D002454:Cell Differentiation; D002779:Cholestasis / Q000378:metabolism; D004195:Disease Models, Animal; D005260:Female; D005315:Fetal Diseases / Q000473:pathology*; D006801:Humans; D051379:Mice; D032383:Myocytes, Cardiac / Q000166:cytology* / Q000378:metabolism; D011247:Pregnancy; D011249:Pregnancy Complications, Cardiovascular; D013997:Time Factors
D001647:Bile Acids and Salts; D002118:Calcium