Effect of microwave irradiation on covalent ligand-DNA interactions.
A 30 second burst of microwave irradiation at an energy level insufficient to cause DNA denaturation or damage drives the covalent reaction between pyrrolobenzodiazepine (PBD) antitumour agents and double-stranded or hairpin oligonucleotides to completion, a process that normally takes between 3-24 hours and thus offering the opportunity for higher-throughput screening of covalent-binding DNA-interactive agents.
Gene Targeted Drug Design Research Group, School of Pharmacy, University of London, London, UK.
Khondaker M
KM
Rahman
David E
DE
Thurston
D000970:Antineoplastic Agents / Q000737:chemistry / Q000378:metabolism* / Q000528:radiation effects; D001569:Benzodiazepines / Q000737:chemistry / Q000378:metabolism* / Q000528:radiation effects; D001665:Binding Sites; D002851:Chromatography, High Pressure Liquid; D004247:DNA / Q000737:chemistry / Q000378:metabolism* / Q000528:radiation effects*; D008024:Ligands; D008872:Microwaves; D008958:Models, Molecular; D011758:Pyrroles / Q000737:chemistry / Q000378:metabolism* / Q000528:radiation effects; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013997:Time Factors
D000970:Antineoplastic Agents; D008024:Ligands; D011758:Pyrroles; C438462:pyrrolo(2,1-c)(1,4)benzodiazepine; D001569:Benzodiazepines; D004247:DNA