Determination of metoclopramide in human plasma using hydrophilic interaction chromatography with tandem mass spectrometry.
For the rapid, selective and sensitive analysis of metoclopramide in human plasma, hydrophilic interaction chromatography with electrospray ionization tandem mass spectrometric (HILIC/MS/MS) method was developed. This method involved liquid-liquid extraction with dichloromethane followed by separation on an Atlantis HILIC silica column using the mobile phase of acetonitrile-ammonium formate (100 mM, pH 6.5) (85:15, v/v). Analytes were quantified using electrospray ionization mass spectrometry in the selected reaction monitoring mode. The standard curve was linear (r(2)- 0.998) over the concentration range of 2.00 - 150 ng/mL using 50 microL of plasma sample. The coefficient of variation and relative error for intra- and inter-assay at four QC levels were 1.8 - 7.7% and -7.5 to 3.6%, respectively. The matrix effect for metoclopramide and levosulpiride (internal standard) was practically absent. The present method was successfully applied to the pharmacokinetic study of metoclopramide after oral dose of metoclopramide hydrochloride (10mg) to male healthy volunteers.
Lee
Hye Won
HW
National Research Laboratory for Drug Metabolism and Bioanalysis, College of Pharmacy, Wonkwang University, Iksan 570-749, Chonbuk, Republic of Korea.
Ji
Hye Young
HY
Kim
Hoe Yoon
HY
Park
Eun-Seok
ES
Lee
Kang Choon
KC
Lee
Hye Suk
HS
D002853:Chromatography, Liquid / Q000379:methods*; D006801:Humans; D008787:Metoclopramide / Q000097:blood* / Q000493:pharmacokinetics; D012015:Reference Standards; D012016:Reference Values; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D053719:Tandem Mass Spectrometry / Q000379:methods*
D008787:Metoclopramide