Sulfite determination by a biosensor based on bay leaf tissue homogenate: very simple and economical method.
Of all the food additives for which the FDA has received adverse reaction reports, the ones that most closely resemble true allergens are sulfur-based preservatives. Sulfites are used primarily as antioxidants to prevent or reduce discoloration of light-colored fruits and vegetables, such as dried apples and potatoes, and to inhibit the growth of microorganisms in fermented foods such as wine. This work aims to prepare an electrochemical biosensor based on bay leaf tissue homogenate that contains polyphenol oxidase enzyme abundantly for sulfite detection in foods. The principle of the biosensor is based on the inhibition effect of sulfites on polyphenol oxidase in the bioactive layer. Optimum conditions for the biosensor, such as temperature and pH, were investigated. Some stability parameters of the biosensor were also identified. The biosensor showed a linear calibration graph in the range of 25-100 microM sulfite. The biosensor presents a very simple, economical, reliable, and feasible method for sulfite detection in foods.
Teke
Mustafa
M
Muğla University, Science and Art Faculty, Chemistry Department, Muğla, Turkey. mteke@mu.edu.tr
Sezgintürk
Mustafa Kemal
MK
Dinçkaya
Erhan
E
D055162:Biocatalysis; D015374:Biosensing Techniques / Q000191:economics* / Q000379:methods*; D002138:Calibration; D004156:Catechol Oxidase / Q000737:chemistry / Q000378:metabolism; D004563:Electrochemistry; D004795:Enzyme Stability; D005504:Food Analysis / Q000191:economics / Q000379:methods; D005520:Food Preservatives / Q000032:analysis; D006863:Hydrogen-Ion Concentration; D027442:Laurus / Q000201:enzymology*; D010936:Plant Extracts; D013447:Sulfites / Q000032:analysis*; D013696:Temperature
D005520:Food Preservatives; D010936:Plant Extracts; D013447:Sulfites; D004156:Catechol Oxidase