Mating system and seed variation of Acacia hybrid (A. mangium x A. auriculiformis).
The mating system and seed variation of Acacia hybrid (A. mangium x A. auriculiformis) were studied using allozymes and random amplified polymorphic DNA (RAPD) markers, respectively. Multi-locus outcrossing rate estimations indicated that the hybrid was predominantly outcrossed (mean+/- s.e. t(m) = 0.86+/-0.01). Seed variation was investigated using 35 polymorphic RAPD fragments. An analysis of molecular variance (AMOVA) revealed the highest genetic variation among seeds within a pod (66%-70%), followed by among pods within inflorescence (29%-37%), and the least variation among inflorescences within tree (1%). In addition, two to four RAPD profiles could be detected among seeds within pod. Therefore, the results suggest that a maximum of four seeds per pod could be sampled for the establishment of a mapping population for further studies.
Genetic Unit, Forest Biotechnology and Pharmaceutical Programme, Forest Research Institute Malaysia,52109 Kepong, Malaysia. chinhong@frim.gov.my
Chin-Hong
CH
Ng
Soon-Leong
SL
Lee
Kevin Kit-Siong
KK
Ng
Norwati
N
Muhammad
Wickneswari
W
Ratnam
D000045:Acacia / Q000235:genetics*; D003433:Crosses, Genetic; D018744:DNA, Plant / Q000737:chemistry; D017343:Genes, Plant; D014644:Genetic Variation; D005828:Genetics, Population; D007527:Isoenzymes / Q000235:genetics; D012639:Seeds / Q000235:genetics*
D018744:DNA, Plant; D007527:Isoenzymes