[Molecular detection of the BCR-ABL gen by RT-PCR in Costa Rican children with leukemia].
Many leukemias could have chromosomic translocations and according to the transcripts formed by the genes involved, the patients present an specific phenotype of the leukemia. We show the first results of the investigation of the gen BCR-ABL using RT-PCR, in order to look for the t(9;22)(q34;q11) in pediatric leukemic children. We studied in total 55 patients, 6 (10.9%) of them were positive for that translocation. Two (3.63%) of the positive children had ALL and the other 4 (7.27%) presented CML, the genotyping analysis of the transcript was studied in these children. With the introduction of this methodology as part of the routine studies, the leukemic children could get in the future an specific diagnosis, that will be important to classify them in prognostic categories and to improve the detection of minimal residual disease.
Centro de Investigación en Hematología y Trastomos Afines, Universidad de Costa Rica. gerardo.jimenez@ucr.ac.cr
Gerardo
G
Jiménez-Arce
Juan
J
Carrillo
Mario
M
Chaves
Rafael
R
Jiménez
Mario
M
Vargas
Liliana
L
Campos
Ana
A
de la Guardia
Berta
B
Valverde
D014408:Biomarkers, Tumor / Q000235:genetics*; D002648:Child; D016044:Fusion Proteins, bcr-abl / Q000235:genetics*; D016313:Genes, abl / Q000235:genetics*; D005838:Genotype; D006801:Humans; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000175:diagnosis / Q000235:genetics*; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000175:diagnosis / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction
D014408:Biomarkers, Tumor; D016044:Fusion Proteins, bcr-abl