Analysis of PFOA in dosed CD-1 mice. Part 2. Disposition of PFOA in tissues and fluids from pregnant and lactating mice and their pups.
Previous studies in mice with multiple gestational exposures to perfluorooctanoic acid (PFOA) demonstrate numerous dose dependent growth and developmental effects which appeared to worsen if offspring exposed in utero nursed from PFOA-exposed dams. To evaluate the disposition of PFOA in the pregnant and lactating dam and her offspring, time-pregnant CD-1 mice received a single 0, 0.1, 1, or 5mg PFOA/kg BW dose (n=25/dose group) by gavage on gestation day 17. Maternal and pup fluids and tissues were collected over time. Pups exhibited significantly higher serum PFOA concentrations than their respective dams, and their body burden increased after birth until at least postnatal day 8, regardless of dose. The distribution of milk:serum PFOA varied by dose and time, but was typically in excess of 0.20. These data suggest that milk is a substantial PFOA exposure route in mice and should be considered in risk assessment modeling designs for this compound.
Reproductive Toxicology Division, National Health and Environmental Effects Research Laboratory, ORD, U.S. EPA, MD-67, Research Triangle Park, NC 27711, USA. Electronic address: fenton.suzanne@epa.gov.
Suzanne E
SE
Fenton
Oakridge Institute for Science and Education (ORISE) Research Participant, Human Exposure and Atmospheric Sciences Division, National Exposure Research Laboratory, ORD, U.S. EPA, Research Triangle Park, NC 27711, USA.
Jessica L
JL
Reiner
Oakridge Institute for Science and Education (ORISE) Research Participant, Human Exposure and Atmospheric Sciences Division, National Exposure Research Laboratory, ORD, U.S. EPA, Research Triangle Park, NC 27711, USA.
Shoji F
SF
Nakayama
Human Exposure and Atmospheric Sciences Division, National Exposure Research Laboratory, ORD, U.S. EPA, Research Triangle Park, NC 27711, USA.
Amy D
AD
Delinsky
Reproductive Toxicology Division, National Health and Environmental Effects Research Laboratory, ORD, U.S. EPA, MD-67, Research Triangle Park, NC 27711, USA.
Jason P
JP
Stanko
Reproductive Toxicology Division, National Health and Environmental Effects Research Laboratory, ORD, U.S. EPA, MD-67, Research Triangle Park, NC 27711, USA.
Erin P
EP
Hines
Reproductive Toxicology Division, National Health and Environmental Effects Research Laboratory, ORD, U.S. EPA, MD-67, Research Triangle Park, NC 27711, USA; Curriculum in Toxicology, University of North Carolina, Chapel Hill, NC 27599, USA.
Sally S
SS
White
Human Exposure and Atmospheric Sciences Division, National Exposure Research Laboratory, ORD, U.S. EPA, Research Triangle Park, NC 27711, USA.
Andrew B
AB
Lindstrom
Human Exposure and Atmospheric Sciences Division, National Exposure Research Laboratory, ORD, U.S. EPA, Research Triangle Park, NC 27711, USA.
Mark J
MJ
Strynar
Oakridge Institute for Science and Education (ORISE) Research Participant, Human Exposure and Atmospheric Sciences Division, National Exposure Research Laboratory, ORD, U.S. EPA, Research Triangle Park, NC 27711, USA.
Syrago-Styliani E
SE
Petropoulou
D000653:Amniotic Fluid / Q000737:chemistry; D000818:Animals; D000831:Animals, Newborn; D001774:Blood Chemical Analysis / Q000379:methods; D001835:Body Weight / Q000187:drug effects*; D002210:Caprylates / Q000032:analysis* / Q000378:metabolism / Q000633:toxicity; D004305:Dose-Response Relationship, Drug; D004785:Environmental Pollutants / Q000032:analysis* / Q000378:metabolism / Q000633:toxicity; D005260:Female; D005466:Fluorocarbons / Q000032:analysis* / Q000378:metabolism / Q000633:toxicity; D007774:Lactation; D018811:Maternal Exposure; D051379:Mice; D008815:Mice, Inbred Strains; D008892:Milk / Q000737:chemistry*; D011247:Pregnancy; D011297:Prenatal Exposure Delayed Effects / Q000097:blood / Q000139:chemically induced* / Q000378:metabolism / Q000652:urine; D016482:Urinalysis
D002210:Caprylates; D004785:Environmental Pollutants; D005466:Fluorocarbons; C023036:perfluorooctanoic acid