Real-time high resolution melting PCR for identification of the Swedish variant of Chlamydia trachomatis.
We developed a real-time High Resolution Melting PCR to identify the new Swedish variant of Chlamydia trachomatis ncCT. Of 1191 urogenital specimens C. trachomatis-positive by an omp1 real-time PCR, collected in France in 2007-2008, 1128 gave an interpretable profile corresponding to the wild-type strain; no nvCT was found. This test can be used on selected C. trachomatis-positive samples to monitor the nvCT spread.
Raherison
Sophie
S
Laboratoire de Bactériologie EA3671, Centre National de Référence des infections à Chlamydia, Université Victor Segalen Bordeaux 2, France.
Clerc
Maithé
M
Trombert
Sabine
S
Cado
Sylvie
S
Normandin
Françoise
F
Bébéar
Christiane
C
de Barbeyrac
Bertille
B
D002690:Chlamydia Infections / Q000382:microbiology*; D002692:Chlamydia trachomatis / Q000235:genetics / Q000302:isolation & purification*; D014644:Genetic Variation; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D013548:Sweden; D044366:Transition Temperature