An exopolysaccharide produced by Geobacillus thermodenitrificans strain B3-72: antiviral activity on immunocompetent cells.
The immunomodulatory and antiviral effects of an extracellular polysaccharide (EPS-2), produced by a strain of Geobacillus thermodenitrificans isolated from a shallow marine vent of Vulcano Island (Italy), were evaluated. In the present study, we show for the first time that EPS-2 treatment hinder HSV-2 replication in human peripheral blood mononuclear cells (PBMC) but not in WISH cells. In fact, high levels of IFN-alpha, IL-12, IFN-gamma, TNF-alpha, IL-18 were detected in supernatants of EPS-2 treated PBMC. Moreover, this effect was dose-dependent. Taken together, our results confirm that the immunological disorders determined by HSV-2 could be partially restored by treatment with EPS-2.
Department of Surgical Science, Unit of Microbiology, University of Messina, Via Consolare Valeria 1, 98125 Messina, Italy.
Adriana
A
Arena
Concetta
C
Gugliandolo
Giovanna
G
Stassi
Bernadette
B
Pavone
Daniela
D
Iannello
Giuseppe
G
Bisignano
Teresa Luciana
TL
Maugeri
D001406:Bacillaceae / Q000276:immunology*; D002478:Cells, Cultured; D016207:Cytokines / Q000276:immunology / Q000378:metabolism; D006561:Herpes Simplex / Q000276:immunology* / Q000821:virology; D018258:Herpesvirus 2, Human / Q000187:drug effects* / Q000502:physiology; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000187:drug effects / Q000276:immunology; D011135:Polysaccharides, Bacterial / Q000276:immunology / Q000302:isolation & purification / Q000494:pharmacology*; D014779:Virus Replication / Q000187:drug effects* / Q000276:immunology
D016207:Cytokines; D011135:Polysaccharides, Bacterial; C085953:exopolysaccharide, Bacillus