Long chain perfluorinated alkyl acids derivatisation and identification in biota and abiota matrices using gas chromatography.
An analytical method involving derivatisation of perfluorocarboxylic acids for their analysis in abiotic and biotic matrices is presented. Derivatisation of the acid group to form a suitable alkyl ester provided a suitable compound for mass spectrometric detection in gas chromatography/mass spectroscopy (GC/MS) instrumental analysis. The acid is esterified by an alkyl halide i.e. benzyl bromide as the alkylating agent for perfluorocarboxylic acids quantification in fish and water by GC/MS. The gas chromatography method can be applied in the analysis perfluoro alkyl acids in water and biological matrices, especially where high levels of these compounds are expected. Typical values for precision obtained were 0.1%-10.0% with concentrations ranging from 0.2 to 1 microg/mL). Results demonstrate that GC/MS can supplement liquid chromatographic/mass spectroscopy method for quantification of fluorocarboxylic acid surfactants. The result indicates that there is need for more research on method analysis of perfluorinated acids in environmental matrices.
IWW Rheinisch-Westfälisches Institut für Wasserforschung gemeinnützige GmbH, 64584 Biebesheim, Germany. fraora@yahoo.com
Francis
F
Orata
Natalia
N
Quinete
Rolf-Dieter
RD
Wilken
D000143:Acids / Q000737:chemistry; D000818:Animals; D002138:Calibration; D002849:Chromatography, Gas / Q000379:methods*; D004951:Esterification; D005399:Fishes; D005466:Fluorocarbons / Q000032:analysis* / Q000737:chemistry; D008401:Gas Chromatography-Mass Spectrometry; D014874:Water Pollutants, Chemical / Q000032:analysis*
D000143:Acids; D005466:Fluorocarbons; D014874:Water Pollutants, Chemical