Chemical composition, antibacterial and antioxidant activities of leaf essential oil and extracts of Metasequioa glyptostroboides Miki ex Hu.
The aims of this study were to analyze the chemical composition of leaf essential oil of Metasequioa glyptostroboides Miki, and to test the efficacy of oil and extracts (hexane, chloroform, ethyl acetate and methanol) against food spoilage and food-borne pathogenic bacteria and their antioxidant activity. The GC-MS analysis revealed 49 compounds representing 94.62% of the total oil containing 2-butaneone (30.6%), cyclopentane (15.1%), beta-myrcene (13.29%), cyclobutane (7.67%), furan (3%), valeramide (2.81%), borneol (1.2%), beta-farnesene (1.67%), thymol (1.44%) and alpha-pinene (1.46%) as major components. The oil (1000 microg/disc), and extracts (1500 microg/disc) exhibited promising antibacterial effect as a diameter of zones of inhibition (10-18 and 7-13 mm), respectively. MIC values of oil and the extracts were ranged 125-2000 and 250 to <2000 microg/ml, respectively. Also the oil had strong antibacterial effect on the viable counts. Scanning electron microscopic study demonstrated potential detrimental effect of the oil on the morphology of S. aureus KCTC1916. The free radical scavenging activities of the oil and ethyl acetate extract were found to be 11.32 and 19.12 microg/ml, respectively. Also the ethyl acetate extract revealed the highest phenolic contents (85.17 mg/g of dry wt) as compared to the other extracts.
Bajpai
Vivek K
VK
Department of Biotechnology, College of Engineering, Daegu University, Kyoungsan, Kyoungbook 712-714, Republic of Korea.
Al-Reza
Sharif M
SM
Choi
Ung Kyu
UK
Lee
Jong Hwi
JH
Kang
Sun Chul
SC
D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000494:pharmacology*; D000975:Antioxidants / Q000737:chemistry* / Q000494:pharmacology*; D001713:Biphenyl Compounds / Q000737:chemistry; D015169:Colony Count, Microbial; D005516:Food Microbiology; D005519:Food Preservation; D005609:Free Radicals / Q000737:chemistry; D008401:Gas Chromatography-Mass Spectrometry; D006090:Gram-Negative Bacteria / Q000187:drug effects; D006094:Gram-Positive Bacteria / Q000187:drug effects; D007202:Indicators and Reagents; D008826:Microbial Sensitivity Tests; D008855:Microscopy, Electron, Scanning; D009822:Oils, Volatile / Q000737:chemistry* / Q000494:pharmacology*; D010636:Phenols / Q000032:analysis / Q000737:chemistry; D010851:Picrates / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry; D018515:Plant Leaves / Q000737:chemistry; D032487:Sequoia / Q000737:chemistry*
D000900:Anti-Bacterial Agents; D000975:Antioxidants; D001713:Biphenyl Compounds; D005609:Free Radicals; D007202:Indicators and Reagents; D009822:Oils, Volatile; D010636:Phenols; D010851:Picrates; D010936:Plant Extracts; C004931:1,1-diphenyl-2-picrylhydrazyl