CAT C-262T and GPX1 Pro198Leu polymorphisms in a Turkish population.
Oxidative stress is believed to play an important role in the pathogenesis of considerable number of complex diseases. The antioxidant enzymes catalase (CAT) and glutathione peroxidase (GPX) are important components of cell defense against oxidative stress, and polymorphisms in the genes which regulate their expression may contribute to differences in susceptibility of individuals to oxidative damage caused by reactive oxygen species. The aim of this study was to assess the distribution of CAT C-262T and GPX1 Pro198Leu genotypic variants in a Turkish population. Genotyping analyses of CAT and GPX1 were conducted in 250 unrelated, healthy volunteers by the PCR-RFLP assay. The allele frequencies were 0.784 (C) and 0.216 (T) for CAT and 0.636 (C) and 0.364 (T) for GPX1 Pro198Leu. The genotype frequencies were 0.632 (CC), 0.304 (CT), and 0.064 (TT) for CAT and 0.416 (CC), 0.44 (CT), and 0.144 (TT) for GPX1 Pro198Leu. The genotype frequencies did not deviate from Hardy-Weinberg equilibrium. The results are compared with those of other reported populations. They showed marked ethnic group differences.
Department of Toxicology, Faculty of Pharmacy, Ankara University, Ankara, Turkey. suzen@pharmacy.ankara.edu.tr
H Sinan
HS
Suzen
Emel
E
Gucyener
Ozgul
O
Sakalli
Zuhal
Z
Uckun
Gulcin
G
Kose
Duygu
D
Ustel
Yalcin
Y
Duydu
D000293:Adolescent; D000328:Adult; D000368:Aged; D016022:Case-Control Studies; D002374:Catalase / Q000235:genetics*; D005260:Female; D005787:Gene Frequency / Q000235:genetics; D005838:Genotype; D005979:Glutathione Peroxidase / Q000235:genetics*; D006801:Humans; D007930:Leucine / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D011392:Proline / Q000235:genetics*; D014421:Turkey; D055815:Young Adult; D000094765:Glutathione Peroxidase GPX1
D011392:Proline; D002374:Catalase; D005979:Glutathione Peroxidase; D007930:Leucine; D000094765:Glutathione Peroxidase GPX1; C000722067:GPX1 protein, human