Elevated CO2 and O3 effects on fine-root survivorship in ponderosa pine mesocosms.
Atmospheric carbon dioxide (CO(2)) and ozone (O(3)) concentrations are rising, which may have opposing effects on tree C balance and allocation to fine roots. More information is needed on interactive CO(2) and O(3) effects on roots, particularly fine-root life span, a critical demographic parameter and determinant of soil C and N pools and cycling rates. We conducted a study in which ponderosa pine (Pinus ponderosa) seedlings were exposed to two levels of CO(2) and O(3) in sun-lit controlled-environment mesocosms for 3 years. Minirhizotrons were used to monitor individual fine roots in three soil horizons every 28 days. Proportional hazards regression was used to analyze effects of CO(2), O(3), diameter, depth, and season of root initiation on fine-root survivorship. More fine roots were produced in the elevated CO(2) treatment than in ambient CO(2). Elevated CO(2), increasing root diameter, and increasing root depth all significantly increased fine-root survivorship and median life span. Life span was slightly, but not significantly, lower in elevated O(3), and increased O(3) did not reduce the effect of elevated CO(2). Median life spans varied from 140 to 448 days depending on the season of root initiation. These results indicate the potential for elevated CO(2) to increase the number of fine roots and their residence time in the soil, which is also affected by root diameter, root depth, and phenology.
National Health & Environmental Effects Research Laboratory, US Environmental Protection Agency, Corvallis, OR 97333, USA. phillips.donald@epa.gov
Donald L
DL
Phillips
Mark G
MG
Johnson
David T
DT
Tingey
Marjorie J
MJ
Storm
D001272:Atmosphere / Q000737:chemistry*; D002244:Carbon / Q000032:analysis; D002245:Carbon Dioxide / Q000032:analysis*; D008136:Longevity / Q000502:physiology; D009584:Nitrogen / Q000032:analysis; D010126:Ozone / Q000032:analysis*; D041604:Pinus ponderosa / Q000254:growth & development*; D018517:Plant Roots / Q000254:growth & development*; D012987:Soil / Q000032:analysis; D016019:Survival Analysis; D014741:Video Recording
D012987:Soil; D002245:Carbon Dioxide; D010126:Ozone; D002244:Carbon; D009584:Nitrogen