Positive charged polymer as a probe for DNA determination by resonance light scattering.
Electrostatic interactions of calf thymus DNA (ct-DNA) with a positive charged polymer were investigated based on measurements of enhanced resonance light scattering (RLS) signals, and were proved by scanning electron microscopy (SEM). The optimal pH value was 7.0 in a phosphate-buffered saline solution (PBS) and the maximum RLS peak located at 344.8 nm. The RLS signal enhancement (DeltaI(RLS)) could be linearly correlated with the concentration of ct-DNA in the range of 0.0900 to 2.70 microg mL(-1), and the detection limit (S/N = 3) was 17.0 ng mL(-1). The binding ratio and the binding constant between DNA and polymer were also provided.
Key Laboratory on Luminescence and Real-Time Analysis, Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Beibei, Chongqing 400715, PR China.
Juan
J
Du
Huzhi
H
Zheng
Chengzhi
C
Huang
Congying
C
Wen
Jianyu
J
Hao
Yufei
Y
Hu
Dongbo
D
Zhou
Li
L
Liu
D000818:Animals; D002138:Calibration; D002417:Cattle; D004247:DNA / Q000032:analysis* / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D008027:Light; D009994:Osmolar Concentration; D011145:Polyvinyls / Q000737:chemistry*; D012542:Scattering, Radiation; D055672:Static Electricity
D011145:Polyvinyls; C027968:poly-4-vinyl-N-ethylpyridine; D004247:DNA; C427203:calf thymus DNA