HBV-DNA in hemodialysis patients infected by HCV.
End-stage renal disease patients on chronic hemodialysis (HD) patients are at risk for both hepatitis B virus (HBV) and hepatitis C virus (HCV) infection, and they may coexist. To determine the prevalence and clinical impact of HBV and HCV infection, we studied poly chain reaction (PCR) and reverse transcription (RT)-PCR on the blood samples of 90 HD patients in Kerman, Iran. ELISA test was used to detect anti-HBc, anti-HBs and HBsAg. We found that 30 out of 90 (33.3%) patients were PCR-RT-PCR positive for HCV-RNA. No HBV-DNA (0%) was detected through the PCR study in both positive and negative HCV-RNA patient groups. Though none of the samples was HBsAg positive, 10 (33.3%) HCV-RNA positive patients were anti-HBc positive, and 12 (40.7%) were anti-HBs positive. We conclude that prevalence of hepatitis C infection is high in HD patients in our region, but not associated with active HBV infection.
Department of Microbiology and Immunology, Rafsanjan University of Medical Science, Iran. kazemi24@yahoo.com
Mohammad Kazemi
MK
Arababadi
Gholamhossein
G
Hassanshahi
Hassan
H
Yousefi
D000368:Aged; D003430:Cross-Sectional Studies; D004279:DNA, Viral / Q000097:blood*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D016174:Hepacivirus / Q000235:genetics*; D006509:Hepatitis B / Q000150:complications / Q000175:diagnosis* / Q000453:epidemiology; D006510:Hepatitis B Antibodies / Q000097:blood; D006514:Hepatitis B Surface Antigens / Q000097:blood; D006515:Hepatitis B virus / Q000235:genetics* / Q000276:immunology; D006526:Hepatitis C / Q000150:complications / Q000175:diagnosis* / Q000453:epidemiology; D006801:Humans; D007492:Iran / Q000453:epidemiology; D007676:Kidney Failure, Chronic / Q000453:epidemiology / Q000628:therapy* / Q000821:virology; D008297:Male; D008875:Middle Aged; D015995:Prevalence; D006435:Renal Dialysis; D020133:Reverse Transcriptase Polymerase Chain Reaction
D004279:DNA, Viral; D006510:Hepatitis B Antibodies; D006514:Hepatitis B Surface Antigens