Surface immobilization of PSI using vitamin K1-like molecular wires for fabrication of a bio-photoelectrode.
We present a bio-photoelectrode made of photosystem I complex (PSI) of cyanobacteria, Thermosynechococcus elongatus, and molecular wires. The newly designed and synthesized molecular wire similar to vitamin K1 (VK1) has a naphthoquinone moiety that can connect to PSI, and a terpyridine moiety for connection to a Co(II) ion. Surface immobilization of PSI and molecular wires was performed by a self-assembled monolayer (SAM) formation process at indium tin oxide (ITO) followed by stepwise coordination reactions. The PSI-immobilized ITO showed a photocurrent action spectrum, whose profile was consistent with that of PSI. We have demonstrated a new method for the immobilization of PSI on the electrode surface, useful in the construction of phovoltaic cells or photon-sensing devices.
Department of Chemistry School of Sciences, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.
Mariko
M
Miyachi
Yoshinori
Y
Yamanoi
Tetsu
T
Yonezawa
Hiroshi
H
Nishihara
Michinao
M
Iwai
Masae
M
Konno
Masako
M
Iwai
Yasunori
Y
Inoue
D015374:Biosensing Techniques / Q000295:instrumentation; D000458:Cyanobacteria; D004566:Electrodes; D055767:Immobilized Proteins / Q000737:chemistry*; D008958:Models, Molecular; D055668:Photochemical Processes; D045331:Photosystem I Protein Complex / Q000737:chemistry*; D013053:Spectrophotometry; D017971:Tin Compounds / Q000737:chemistry; D010837:Vitamin K 1 / Q000737:chemistry*
D055767:Immobilized Proteins; D045331:Photosystem I Protein Complex; D017971:Tin Compounds; C109984:indium tin oxide; D010837:Vitamin K 1