Preparative enzymatic synthesis of polyprenyl-pyrophosphoryl-N-acetylglucosamine, an essential lipid intermediate for the biosynthesis of various bacterial cell envelope polymers.
The WecA transferase is an integral membrane protein and a member of the polyprenyl phosphate N-acetylhexosamine-1-phosphate transferase superfamily. It initiates the biosynthesis of various bacterial cell envelope components such as the lipopolysaccharide O-antigen. We report on the first large-scale enzymatic synthesis, purification, and characterization of the undecaprenyl-pyrophosphoryl-N-acetylglucosamine product of the WecA transferase. This is an essential lipid intermediate for the biosynthesis of various bacterial cell envelope components. Its availability in a pure form will allow the biochemical and structural characterization of the various enzymes requiring it as a substrate for the synthesis of cell wall polymers.
Al-Dabbagh
Bayan
B
Univ Paris-Sud, UMR 8619, Institut de Biochimie et de Biophysique Moléculaire et Cellulaire, Orsay 91405, France.
Blanot
Didier
D
Mengin-Lecreulx
Dominique
D
Bouhss
Ahmed
A
D000117:Acetylglucosamine / Q000031:analogs & derivatives* / Q000096:biosynthesis / Q000302:isolation & purification; D055162:Biocatalysis; D002473:Cell Wall / Q000378:metabolism; D029968:Escherichia coli Proteins / Q000378:metabolism*; D011106:Polyisoprenyl Phosphates / Q000096:biosynthesis* / Q000302:isolation & purification; D011108:Polymers / Q000737:chemistry / Q000378:metabolism*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D017855:Transferases (Other Substituted Phosphate Groups) / Q000378:metabolism*
D029968:Escherichia coli Proteins; D011106:Polyisoprenyl Phosphates; D011108:Polymers; C543417:polyprenyl-pyrophosphoryl-N-acetylglucosamine; D017855:Transferases (Other Substituted Phosphate Groups); C083617:wecA protein, E coli; D000117:Acetylglucosamine