Label-free electrochemical detection of the p53 core domain protein on its antibody immobilized electrode.
We report quantitative results on interactions between a tumor suppressor protein, p53, also known as a prognostic cancer marker, and its antibody. The p53 antibody molecules immobilized on an (R)-lipo-diaza-18-crown-6 self-assembled monolayer (SAM)-modified gold disk electrode were shown to effectively capture the p53 protein by Western blot, quartz crystal microbalance, and electrochemical impedance experiments. The p53 protein thus captured modulated the ability of the electrode for charge transfer to and from a redox probe in the solution in a p53 concentration range of approximately 0.1-30 microg/mL. The same interaction was also observed in the human embryonic kidney cell lysate, demonstrating that the SAM-modified electrode can serve as a selective platform for electrochemically monitoring the cellular p53 concentration.
Department of Chemistry, Center for Integrated Molecular Systems, Pohang University of Science and Technology, Pohang, Gyeongbuk 790-784, Korea.
Jongchan
J
Yeo
Jin-Young
JY
Park
Won Jin
WJ
Bae
Yoon Suk
YS
Lee
Byeang Hyean
BH
Kim
Yunje
Y
Cho
Su-Moon
SM
Park
D055910:Antibodies, Immobilized / Q000737:chemistry* / Q000276:immunology; D002460:Cell Line; D043844:Crown Ethers / Q000737:chemistry; D004563:Electrochemistry / Q000379:methods*; D004566:Electrodes; D006046:Gold / Q000737:chemistry; D006801:Humans; D010084:Oxidation-Reduction; D017434:Protein Structure, Tertiary; D011994:Recombinant Proteins / Q000032:analysis / Q000302:isolation & purification; D016159:Tumor Suppressor Protein p53 / Q000032:analysis* / Q000276:immunology / Q000302:isolation & purification
D055910:Antibodies, Immobilized; D043844:Crown Ethers; D011994:Recombinant Proteins; D016159:Tumor Suppressor Protein p53; C015762:18-crown-6; D006046:Gold