A precise reconstruction of the emergence and constrained radiations of Escherichia coli O157 portrayed by backbone concatenomic analysis.
Single nucleotide polymorphisms (SNPs) in stable genome regions provide durable measurements of species evolution. We systematically identified each SNP in concatenations of all backbone ORFs in 7 newly or previously sequenced evolutionarily instructive pathogenic Escherichia coli O157:H7, O157:H(-), and O55:H7. The 1,113 synonymous SNPs demonstrate emergence of the largest cluster of this pathogen only in the last millennium. Unexpectedly, shared SNPs within circumscribed clusters of organisms suggest severely restricted survival and limited effective population sizes of pathogenic O157:H7, tenuous survival of these organisms in nature, source-sink evolutionary dynamics, or, possibly, a limited number of mutations that confer selective advantage. A single large segment spanning the rfb-gnd gene cluster is the only backbone region convincingly acquired by recombination as O157 emerged from O55. This concatenomic analysis also supports using SNPs to differentiate closely related pathogens for infection control and forensic purposes. However, constrained radiations raise the possibility of making false associations between isolates.
Leopold
Shana R
SR
Department of Pediatrics, Washington University School of Medicine, 660 South Euclid Avenue, Saint Louis, MO 63110, USA.
Magrini
Vincent
V
Holt
Nicholas J
NJ
Shaikh
Nurmohammad
N
Mardis
Elaine R
ER
Cagno
Joseph
J
Ogura
Yoshitoshi
Y
Iguchi
Atsushi
A
Hayashi
Tetsuya
T
Mellmann
Alexander
A
Karch
Helge
H
Besser
Thomas E
TE
Sawyer
Stanley A
SA
Whittam
Thomas S
TS
Tarr
Phillip I
PI
D001483:Base Sequence; D003198:Computer Simulation; D030561:Databases, Nucleic Acid; D019453:Escherichia coli O157 / Q000235:genetics / Q000378:metabolism* / Q000472:pathogenicity / Q000528:radiation effects; D016680:Genome, Bacterial / Q000235:genetics; D008957:Models, Genetic; D008969:Molecular Sequence Data; D020641:Polymorphism, Single Nucleotide / Q000235:genetics; D011995:Recombination, Genetic / Q000235:genetics