Preparation and characterization of protein-loaded polyanhydride microspheres.
Poly(1,3-bis-(p-carboxyphenoxy propane)-co-(sebacic anhydride) (P(CPP-SA)) have the anhydride bonds in copolymer backbone, which are available for degradation on the base of passive hydrolysis. This chemical structure made it degraded within a short time in linear degradation rate. For this property, polyanhydrides are one of the most suitable biodegradable polymers employed as drug carriers. This paper aimed at researching the erosion and degradation of P(CPP-SA) microspheres with CPP/SA monomer ratios of 20:80, 35:65 and 50:50. In vitro protein release from the microspheres was also investigated in this paper. Human serum albumin (HSA) was used as the model protein. In this research, the microspheres degradation and drug release rate from microspheres can be adjusted by altering the CPP/SA ratios of P(CPP-SA). The features of surface erosion were observed in SEM. The structural integrity of HSA extracted from microspheres was detected by gel permeation chromatography, compared with native HSA. The results showed HSA remained its molecule weight after encapsulated.
Key Laboratory of Advanced Technologies of Materials, Ministry of Education, School of Materials Science and Engineering, Southwest Jiaotong University, 610031 Chengdu, People's Republic of China.
Lin
L
Sun
Shaobing
S
Zhou
Weijia
W
Wang
Qiuxiang
Q
Su
Xiaohong
X
Li
Jie
J
Weng
D020341:Absorbable Implants; D002850:Chromatography, Gel; D003652:Decanoic Acids / Q000138:chemical synthesis / Q000737:chemistry / Q000378:metabolism; D004337:Drug Carriers / Q000138:chemical synthesis / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D008863:Microspheres; D008954:Models, Biological; D010316:Particle Size; D049388:Polyanhydrides / Q000138:chemical synthesis* / Q000737:chemistry* / Q000378:metabolism; D011091:Polyesters / Q000138:chemical synthesis / Q000737:chemistry / Q000378:metabolism; D011506:Proteins / Q000008:administration & dosage* / Q000493:pharmacokinetics; D012709:Serum Albumin / Q000008:administration & dosage / Q000493:pharmacokinetics; D013499:Surface Properties; D013997:Time Factors
D003652:Decanoic Acids; D004337:Drug Carriers; D049388:Polyanhydrides; D011091:Polyesters; D011506:Proteins; D012709:Serum Albumin; C055401:decanedioic acid-4,4'-(1,3-propanediylbis(oxy))bis(benzoic acid) copolymer