Acrylamide-induced astrogliotic and apoptotic responses in human astrocytoma cells.
This study was to clarify whether acrylamide (ACR) will induce apoptosis and astrogliosis in an astrocytic cell line in vitro. Different time- and dose-dependent cytotoxic studies were conducted upon neuronal (SH-SY5Y) and glial cell lines (U-1240 MG) under exposure to ACR up to 72h. We showed that SH-SY5Y cells were more sensitive in cytotoxic assays than U-1240 MG cells, and significantly decreased cell viability was observed at concentrations higher than 1mM with increased lactate dehydrogenase leakage observed only at 5 and 10mM in U-1240 MG cells. The ACR-induced apoptotic responses and phosphorylation of p53 protein at Ser15 for U-1240 MG cells were identified at 48h. The increase of glial fibrillary acidic protein (GFAP) as a chemical-induced astrogliotic response was found to be associated with different ACR concentrations and exposure times, particularly at >or=48h of >or=2mM. In addition, immunocytochemical staining at 36h of 5 and 10mM treatments had significantly higher density of GFAP than the control. Thus, ACR-induced effects can be seen in neuronal and astrocytic cells. These results suggest that ACR exposure may lead to apoptotic and astrogliotic effects in human astrocytoma cells in vitro in a time- and dose-dependent manner.
Chen
Jong-Hang
JH
Department of Veterinary Medicine, National Taiwan University, Taipei 106, Taiwan, ROC.
Wu
Kuen-Yuh
KY
Chiu
Ing-Ming
IM
Tsou
Tsui-Chun
TC
Chou
Chin-Cheng
CC
D020106:Acrylamide / Q000008:administration & dosage / Q000633:toxicity*; D017209:Apoptosis / Q000187:drug effects*; D001253:Astrocytes / Q000187:drug effects* / Q000473:pathology; D001254:Astrocytoma / Q000473:pathology; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D005904:Glial Fibrillary Acidic Protein / Q000187:drug effects; D006801:Humans; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D009447:Neuroblastoma / Q000473:pathology*; D010766:Phosphorylation / Q000187:drug effects; D013997:Time Factors; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism
D005904:Glial Fibrillary Acidic Protein; D016159:Tumor Suppressor Protein p53; D020106:Acrylamide; D007770:L-Lactate Dehydrogenase