[Influence of ecoimmunonutrition supplement on intestinal mucosa morphology and gut barrier function in rats after operative stress].
OBJECTIVE
To investigate the effects of ecoimmunonutrition supplement on intestinal microecology, epithelial tight junctions, and barrier function in rats with surgical stress.


METHODS
Seventy SD rats after surgical trauma were randomly divided into four groups:(1) placebo group,(2)total parenteral nutrition(TPN) group,(3)enteral nutrition(EN) group and (4)ecoimmunonutrition (EEN)group respectively. Rats received isocaloric and isonitrogenous nutrition. Nutrients were administered via the neck vein and the needle jejunostomy for five days. The homogenated tissues of liver, lung, and mesenteric lymph nodes were cultured to determine the bacterial translocation rate. The transmembrane binding proteins(occludin) was measured by immunohistochemistry. The ultrastructure and morphology of intestinal epithelial tight junctions in the intestine were observed by electron microscope. The feces in cecum was cultured for anaerobic bacterial growth and analysed.


RESULTS
The amounts of lactobacteria and bifidobacteria in EEN group were significantly higher than those in TPN group(P<0.05). The expression levels of occludin in the intestine was significantly higher in EEN group than that in TPN and EN group. Furthermore, the intestinal epithelial tight junction and microvilli of EEN group were more intact compared with those of TPN group. The bacterial translocation rates of liver, lung and mesenteric lymph nodes were significantly lower in EEN and EN group than those in TPN group(P<0.05).


CONCLUSIONS
Application of ecoimmunonutrition can protect intestinal mucosal barrier in rats with operative stress, increase the expression of occludin, maintain the gut epithelial tight junction, and eliminate gut bacterial translocation.
Zhang
Ming-Ming
MM
Department of General Surgery, West China Hospital, Sichuan University, Chengdu 610041, China.
Cheng
Jing-Qiu
JQ
Zhai
Hong-Jun
HJ
Lu
Yan-Rong
YR
Chen
You-Nan
YN
Wu
Xiao-Ting
XT
D000818:Animals; D004750:Enteral Nutrition; D041981:Gastrointestinal Tract / Q000382:microbiology*; D007413:Intestinal Mucosa / Q000503:physiopathology*; D008297:Male; D019936:Probiotics / Q000627:therapeutic use*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013514:Surgical Procedures, Operative / Q000009:adverse effects*