Development of tissue conditioner capable of binding with anti-microbial protein lactoferrin.
OBJECTIVE
This study focused on the anti-microbial protein human lactoferrin (hLF) commonly found in saliva, and tried to develop biocompatible dental materials that have higher anti-microbial effects.


METHODS
A lyophilized cation exchange resin was added to tissue conditioner at 4 wt% and 8 wt%. The amount of hLF binding to the tissue conditioner and their anti-microbial effect against Candida albicans was investigated. Then their mechanical properties and cytotoxicity were examined.


RESULTS
Tissue conditioner containing cation exchange resin was bound with hLF and had an anti-microbial effect against C. albicans. In addition, their physical properties were sufficient and they were harmless to human fibroblasts.


CONCLUSIONS
The clinical application of cation exchange resin for tissue conditioner can be effective for the prevention and treatment of denture stomatitis and systemic opportunistic infections since it is thought that these materials will increase the local concentration of anti-microbial protein in saliva at the lesion site.
Yamamoto
Daigo
D
Section of Fixed Prosthodontics, Division of Oral Rehabilitation, Faculty of Dental Science, Kyushu University, Higashi-ku, Fukuoka, Japan.
Shinohara
Yoshinori
Y
Nagadome
Hatsumi
H
Terada
Yoshihiro
Y
D001672:Biocompatible Materials; D002176:Candida albicans / Q000187:drug effects*; D002411:Cation Exchange Resins; D002460:Cell Line; D003764:Dental Materials; D025141:Drug Resistance, Fungal; D005347:Fibroblasts / Q000187:drug effects; D005612:Freeze Drying; D006801:Humans; D007781:Lactoferrin / Q000494:pharmacology* / Q000633:toxicity; D009894:Opportunistic Infections / Q000517:prevention & control; D011485:Protein Binding; D012463:Saliva; D013282:Stomatitis, Denture / Q000517:prevention & control; D014016:Tissue Conditioning, Dental
D001672:Biocompatible Materials; D002411:Cation Exchange Resins; D003764:Dental Materials; D007781:Lactoferrin