[Effect of carboxymethylchitosan-carboxymethylcellulose film on colonic anastomosis healing].
OBJECTIVE
To investigate the effects of carboxymethylchitosan-carboxymethylcellulose (CMCH-CMC) film on the adhesion and healing of colonic anastomosis.


METHODS
Sixty-four healthy adult male SD rats was randomly divided into control group and experimental group (n=32). The model of colonic anastomosis was made according to Buckenmaier's method in all rats. The experimental group was treated by wrapping anastomosis with CMCH-CMC film (3 cm x 2 cm) and the control group was not treated. At 7 days and 14 days after operation, the adhesion formation of colonic anastomosis was observed, the tensile strength of the anastomosis was assessed and compared with 6 normal rats, and the hydroxyproline (HP) content of the anastomosis was detected.


RESULTS
There were 3 deaths in the experimental group and 2 deaths in the control group. The adhesive scores of the experimental group on the 7th and 14th postoperative day [(0.50 +/- 0.16) points and (0.45 +/- 0.14) points, (P < 0.05)] were significantly lower than those of the control group [(1.67 +/- 0.15) points and (2.29 +/- 0.18) points, (P < 0.05)], (P < 0.01). Tensile strength were more marked on the 14th postoperative day than on the 7th postoperative day in the control group (P < 0.05), but there was no significant difference between the 7th day and the 14th day in the experimental group. The tensile strength of the control group and the experimental group on the 14th postoperative day [(178.36 +/- 20.10) and (172.74 +/- 22.18) mmHg] were respectively higher than those on the 7th postoperative day [(138.67 +/- 16.65) and (130.81 +/- 18.38) mmHg] (P < 0.01). The tensile strength of the control group and the experimental group on the 7th postoperative day were respectively significantly lower than that of the normal rats (P < 0.01). The level of HP in the anastomosis was significantly higher on the 7th postoperative day in the experimental group [(84.47 +/- 11.87) microg/mg dried weight] than that of the control group [(55.47 +/- 12.89) microg/mg dried weight), (P < 0.05)], but there was no significant difference between the experimental group and the control group on the 14th postoperative day [(146.07 +/- 14.81) microg/mg dried weight, (137.14 +/- 16.81) microg/mg dried weight, (P> 0.05)].


CONCLUSIONS
The CMCH-CMC film can decrease adhesion the formation of colonic anastomosis, but does not interfere with the healing of colonic anastomosis.
Xiao
Haijun
H
Department of Orthopaedics, Fengxian District Center Hospital, Shanghai, PR China.
Hou
Chunlin
C
Xue
Feng
F
D000714:Anastomosis, Surgical / Q000379:methods; D000818:Animals; D001672:Biocompatible Materials; D002266:Carboxymethylcellulose Sodium; D048271:Chitosan / Q000031:analogs & derivatives*; D003106:Colon / Q000601:surgery*; D008297:Male; D011184:Postoperative Period; D051381:Rats; D017207:Rats, Sprague-Dawley; D000267:Tissue Adhesions; D014945:Wound Healing
D001672:Biocompatible Materials; D048271:Chitosan; D002266:Carboxymethylcellulose Sodium