Interleukin-1 system in the human fallopian tube-No spatial but a temporal regulation of mRNA and protein expression.
The human fallopian tube provides the environment for the first 5 days of embryonic development in vivo. The IL-1 system is involved in human embryo implantation. This study aimed to investigate IL-1beta, IL-1ra and IL-1R tI expression within the length of the human fallopian tube on mRNA- and protein-level in samples from proliferative versus secretory phase, postmenopause (PMP) samples and samples from intra- (IUP) and extrauterine pregnancies (EUP) to examine possible spatial and hormonal induced changes (fimbrial, ampullary and isthmic tube segments). On mRNA-level, IL-1beta was expressed in all samples except in PMP. IL-1R tI could be detected in all samples whereas IL-1ra was only expressed in secretory phase and the IUP sample. Immunohistochemically we could detect IL-1beta and IL-1R t1 protein in all proliferative and secretory phase samples with maximum intensity in secretory phase samples whereas IL-1ra was expressed in secretory phase samples only. Overall no spatial but temporal differences possibly due to hormonal changes could be observed suggesting a precise regulation of the IL-1 system, especially for IL-1ra and moreover a stable molecular architecture within the full length of the fallopian tube.
Hess
A P
AP
Department of Gynecology and Obstetrics, Heinrich-Heine-University Medical Center, Duesseldorf, Germany. hess@unikid.de
Baston-Buest
D M
DM
Schanz
A
A
Hirchenhain
J
J
Bielfeld
P
P
Kruessel
J S
JS
D005187:Fallopian Tubes / Q000378:metabolism*; D005260:Female; D005786:Gene Expression Regulation / Q000502:physiology*; D006728:Hormones / Q000494:pharmacology; D006801:Humans; D053590:Interleukin 1 Receptor Antagonist Protein / Q000032:analysis / Q000235:genetics / Q000502:physiology; D007375:Interleukin-1 / Q000032:analysis / Q000235:genetics / Q000502:physiology*; D053583:Interleukin-1beta / Q000032:analysis / Q000235:genetics / Q000502:physiology; D008597:Menstrual Cycle; D017698:Postmenopause; D011247:Pregnancy; D011271:Pregnancy, Ectopic; D012333:RNA, Messenger / Q000032:analysis*; D013997:Time Factors
D006728:Hormones; D053590:Interleukin 1 Receptor Antagonist Protein; D007375:Interleukin-1; D053583:Interleukin-1beta; D012333:RNA, Messenger