Nitric oxide inhibits the replication cycle of porcine parvovirus in vitro.
This study investigated the inhibitory effect and mechanism of nitric oxide (NO) on porcine parvovirus (PPV) replication in PK-15 cells. The results showed that two NO-generating compounds, S-nitroso-L-acetylpenicillamine (SNAP) and L-arginine (LA), at a noncytotoxic concentration could reduce PPV replication in a dose-dependent manner and that this anti-PPV effect could be reversed by the NO synthase (NOS) inhibitor N-nitro-L-arginine methyl ester (L-NAME). By assaying the steps of the PPV life cycle, we also show that NO inhibits viral DNA and protein synthesis. This experiment provides a frame of reference for the study of the anti-viral mechanism of NO.
The College of Animal Science and Veterinary Medicine, Henan Agricultural University, Wenhua Road 95#, 450002, Zhengzhou, Henan, People's Republic of China. weizhanyong@hotmail.com
Zhan-Yong
ZY
Wei
Xue-Bin
XB
Wang
Xiao-Dong
XD
Ning
Ya-Bin
YB
Wang
Hong-Ying
HY
Zhang
Dong-Fang
DF
Wang
Hong-Ying
HY
Chen
Bao-An
BA
Cui
D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology*; D001120:Arginine / Q000494:pharmacology; D002460:Cell Line; D004279:DNA, Viral / Q000096:biosynthesis; D009569:Nitric Oxide / Q000494:pharmacology*; D028323:Parvovirus, Porcine / Q000187:drug effects* / Q000502:physiology; D026423:S-Nitroso-N-Acetylpenicillamine / Q000494:pharmacology; D013552:Swine; D014764:Viral Proteins / Q000096:biosynthesis; D014779:Virus Replication / Q000187:drug effects*
D000998:Antiviral Agents; D004279:DNA, Viral; D014764:Viral Proteins; D009569:Nitric Oxide; D026423:S-Nitroso-N-Acetylpenicillamine; D001120:Arginine