Antimicrobial resistance of Listeria monocytogenes isolated from dairy-based food products.
In this study Listeria monocytogenes (L. monocytogenes) was isolated from three traditionally consumed Lebanese dairy-based food products. One hundred and sixty four samples (45 samples of Baladi cheese, 36 samples of Shankleesh and 83 of Kishk) were collected from the Bekaa Valley in the Northeast region of Lebanon. Suspected Listeria colonies were selected and initially identified by using standard biochemical tests. Initial identification of the positive L. monocytogenes colonies was confirmed at the molecular level by Polymerase Chain Reaction (n=30) and the confirmed isolates were evaluated for their susceptibility to 10 commonly used antimicrobials. All of the 30 isolates were confirmed to be L. monocytogenes yielding a PCR product of approximately 660 base pairs (bp). L. monocytogenes was detected in 26.67%, 13.89% and 7.23% of the Baladi cheese, Shankleesh and Kishk samples, respectively. The highest resistance in L. monocytogenes isolates was noted against oxacillin (93.33%) followed by penicillin (90%). The results provide an indication of the contamination levels of dairy-based foods in Lebanon and highlight the emergence of multi-drug resistant Listeria in the environment.
Biology Department, American University of Beirut, Beirut, Lebanon. sharakeh@gmail.com
Steve
S
Harakeh
Imane
I
Saleh
Omar
O
Zouhairi
Elias
E
Baydoun
Elie
E
Barbour
Nisreen
N
Alwan
D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001483:Base Sequence; D015169:Colony Count, Microbial; D017931:DNA Primers; D003611:Dairy Products / Q000382:microbiology*; D004352:Drug Resistance, Microbial; D008089:Listeria monocytogenes / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification; D008826:Microbial Sensitivity Tests; D016133:Polymerase Chain Reaction
D000900:Anti-Bacterial Agents; D017931:DNA Primers