Sequencing single DNA molecules in real time.
One's enough: The direct observation of a DNA-polymerase-based "sequencing engine" using single-molecule detection recently allowed single-molecule sequencing by synthesis in real time. Nucleotides with a fluorescent marker at the 5'-phosphate unit and zero-mode waveguides are crucial components of this approach, which at last promises low-cost genome-scale sequencing.
Ibach
Jenny
J
Department of Chemical Biology/BCMT, Technische Universität Dortmund, Otto-Hahn-Strasse 6, 44227 Dortmund, Germany.
Brakmann
Susanne
S
D001483:Base Sequence; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism; D005456:Fluorescent Dyes / Q000737:chemistry; D017422:Sequence Analysis, DNA / Q000379:methods*
D005456:Fluorescent Dyes; D004259:DNA-Directed DNA Polymerase