A new method for CE-EC determination of mercaptopurine (MP) in a PMMA biochip with on-chip gold nano-electrode ensemble (GNEE) working and decouple electrodes.
This paper presents a new method for CE-EC determination of Mercaptopurine (MP), one of the most important medicines for inflammatory bowel disease (IBD) and acute lymphoblastic leukemia (ALL) treatment, in a PMMA-based microfluidic chip. A simple and reliable process for preparing the template of gold nanoelectrode ensemble (GNEE) and fabricating the integrated microfluidic chip is reported in the present study. The use of GNEE electrodes for both electric current decoupling and signal sensing in the proposed CE-EC chip not only enhances the signal response but also decreases the background noise during detection. Results show that a good detection limit of 100 nM for detecting mercaptopurine is achieved with the proposed method. In addition, the measured results also shows a good linear response between the detected CE-EC signals and the concentration of MP within the range of 100 nM-10 mM (R2 = 0.989). The proposed microchip device provides a novel and fast detection method for mercaptopurine analysis.
Chen
C M
CM
Institute of Biomedical Engineering, National Cheng Kung University Tainan, 701, Taiwan.
Ho
Y H
YH
Wu
S M
SM
Chang
G L
GL
Lin
C H
CH
D004566:Electrodes; D019075:Electrophoresis, Capillary / Q000379:methods*; D006046:Gold; D015122:Mercaptopurine / Q000032:analysis*; D044085:Microfluidics / Q000295:instrumentation*; D036103:Nanotechnology; D019904:Polymethyl Methacrylate / Q000737:chemistry*
D006046:Gold; D019904:Polymethyl Methacrylate; D015122:Mercaptopurine