Immunotherapy: rAAV2 expressing interleukin-15 inhibits HeLa cell tumor growth in mice.
Human interleukin-15 (hIL15) has anti-tumor activities, but it is not convenient for tumor treatment because of its short half-life. A gene therapy for mouse lung cancer using an adenovirus vector expressing IL15 has been reported. However, adenovirus vector-mediated gene therapy can provoke cellular toxicity and inflammatory reactions. The recombinant adenovirus-associated vector 2 (rAAV2) is safer due to minimal cellular toxicity and immune response. In order to demonstrate that gene therapy can be used safely and successfully for human cancer treatment, the rAAV2 expressing hIL15 gene (rAAV2-hIL15) is applied for human cervical cancer, HeLa cell, in this study. This study successfully demonstrates that rAAV2-hIL15 can express IL15 with bioactivities in vitro and in vivo. In conclusion, our studies show that human cervical cancers are inhibited on animal model with rAAV2-hIL15 treatment and provide a safer and important reference for human cancer gene therapy.
Yiang
Giou-Teng
GT
Institute of Biomedical Nutrition, College of Medicine & Nursing, Hung Kuang University, Sha Lu, Taichung, Taiwan. jtyiang@ms73.hinet.net
Harn
Horng-Jyh
HJ
Yu
Yung-Luen
YL
Hu
Sheng-Chuan
SC
Hung
Yu-Ting
YT
Hsieh
Chia-Jung
CJ
Lin
Shinn-Zong
SZ
Wei
Chyou-Wei
CW
D000818:Animals; D045744:Cell Line, Tumor; D000229:Dependovirus / Q000235:genetics* / Q000378:metabolism; D005260:Female; D015316:Genetic Therapy; D005822:Genetic Vectors / Q000235:genetics*; D006367:HeLa Cells; D006801:Humans; D007167:Immunotherapy; D019409:Interleukin-15 / Q000235:genetics* / Q000378:metabolism; D051379:Mice; D008819:Mice, Nude; D009369:Neoplasms / Q000378:metabolism / Q000628:therapy*
D019409:Interleukin-15