Proteome changes related to the anti-cancer activity of HT29 cells by the treatment of ginsenoside Rd.
Ginseng is a representative herbal medicine in Asia and various pharmacological activities of ginsenoside Rd isolated from ginseng have been reported. However, anti-cancer activity and mechanism of ginsenoside Rd in HT29 colon cancer cell lines were not studied yet. We performed proteomic analysis through two-dimensional gel electrophoresis, MALDI-TOF/TOF-MS and database to identify altered protein induced by ginsenoside Rd treatment in HT29. We can identify fourteen proteins contributed to cell growth inhibition induced by Rd. Proteins involved in the inhibition of mitosis (Stathmin1, Microtubule-associated protein RP/EB family and Stratifin) were significantly up- and down-regulated. And proteins associated with apoptosis (Rho GDP dissociation inhibitor, Tropomyosin1 and Annexin5) were significantly changed. Furthermore, ginsenoside Rd in HT29 was involved in cytoprotection, DNA replication and repair, protein synthesis and degradation, metastasis and mutagenesis. It was supposed that ginsenoside Rd contributed to induce anti-cancer activity by complementary functions of these proteins in colon cancer cells.
Lee
Seo Young
SY
College of Pharmacy and Research Institute of Pharmaceutical Sciences, Seoul National University, Seoul, Korea.
Kim
Geun Tae
GT
Roh
Si Hun
SH
Song
Jin-Su
JS
Kim
Hie-Joon
HJ
Hong
Soon-Sun
SS
Kwon
Sung Won
SW
Park
Jeong Hill
JH
D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D053148:Caspase 3 / Q000235:genetics / Q000378:metabolism; D053938:DNA Fragmentation; D004261:DNA Replication; D015536:Down-Regulation / Q000235:genetics; D015180:Electrophoresis, Gel, Two-Dimensional; D036145:Ginsenosides / Q000494:pharmacology*; D019073:HT29 Cells; D006801:Humans; D006868:Hydrolysis; D007090:Image Interpretation, Computer-Assisted; D007202:Indicators and Reagents; D020543:Proteome / Q000235:genetics*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013778:Tetrazolium Salts; D013844:Thiazoles; D014357:Trypsin / Q000737:chemistry
D000972:Antineoplastic Agents, Phytogenic; D036145:Ginsenosides; D007202:Indicators and Reagents; D020543:Proteome; D013778:Tetrazolium Salts; D013844:Thiazoles; D014357:Trypsin; D053148:Caspase 3; C022616:thiazolyl blue; C049863:ginsenoside Rd