Application of LC/MS to proteomics studies: current status and future prospects.
Liquid chromatography/mass spectrometry (LC/MS) has become a powerful technology in proteomics studies in drug discovery, including target protein characterization and discovery of biomarkers. This review article will describe current LC/MS approaches in protein characterization, including a bottom-up method for protein identification and quantitative proteomics. We will discuss the investigation of protein post-translational modifications such as glycosylation (glycoproteomics) and phosphorylation (phosphoproteomics) using LC/MS. Future trends in LC/MS with respect to proteomics studies will also be illustrated.
Chen
Guodong
G
Schering-Plough Research Institute, 2015 Galloping Hill Road, Kenilworth, NJ 07033, USA. guodong.chen@bms.com
Pramanik
Birendra N
BN
D000595:Amino Acid Sequence; D015415:Biomarkers; D002853:Chromatography, Liquid / Q000379:methods* / Q000639:trends*; D016503:Drug Delivery Systems; D055808:Drug Discovery; D005544:Forecasting; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods* / Q000639:trends*; D008969:Molecular Sequence Data; D011499:Protein Processing, Post-Translational; D040901:Proteomics / Q000379:methods* / Q000639:trends*
D015415:Biomarkers