Yeast, beef and pork extracts counteract Clostridium difficile toxin A enterotoxicity.
Clostridium difficile is responsible for a large proportion of nosocomial cases of antibiotic-associated diarrhoea and pseudomembranous colitis. The present study provides evidence that yeast, beef and pork extracts, ingredients commonly used to grow bacteria, can counteract C. difficile toxin A enterotoxicity in vitro and in vivo. In model intestinal epithelial cells the individual extracts could prevent the toxin A-induced decrease in epithelial barrier function and partially prevented actin disaggregation and cell rounding. Mice with ad libitum access to individual extracts for 1 week had almost complete reduction in toxin A-induced fluid secretion in intestinal loops. Concomitantly, the toxin A-induced expression of the essential proinflammatory mediator Cox-2 was normalized. Moreover this protective effect was also seen when mice received only two doses of extract by intragastric gavage within 1 week. These results show that yeast, beef and pork extracts have the potential to counteract the intestinal pathogenesis triggered by C. difficile toxin A.
Duncan
Peter I
PI
Nestlé Research Center, Lausanne, Switzerland. peter.duncan@rdls.nestle.com
Fotopoulos
Grigorios
G
Pasche
Elisabeth
E
Porta
Nadine
N
Masserey Elmelegy
Isabelle
I
Sanchez-Garcia
Jose-Luis
JL
Bergonzelli
Gabriela E
GE
Corthésy-Theulaz
Irène
I
D000818:Animals; D001427:Bacterial Toxins / Q000037:antagonists & inhibitors / Q000633:toxicity*; D002417:Cattle; D016360:Clostridioides difficile / Q000378:metabolism / Q000472:pathogenicity; D003106:Colon / Q000166:cytology / Q000187:drug effects / Q000473:pathology; D003470:Culture Media / Q000737:chemistry; D017097:Electric Impedance; D004761:Enterocolitis, Pseudomembranous / Q000382:microbiology / Q000473:pathology / Q000628:therapy*; D004768:Enterotoxins / Q000037:antagonists & inhibitors / Q000633:toxicity*; D004847:Epithelial Cells / Q000166:cytology / Q000187:drug effects / Q000473:pathology; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects / Q000473:pathology; D006801:Humans; D008297:Male; D008461:Meat Products; D051379:Mice; D008810:Mice, Inbred C57BL; D044623:Nutrition Therapy; D013552:Swine; D015003:Yeasts / Q000737:chemistry*
D001427:Bacterial Toxins; D003470:Culture Media; D004768:Enterotoxins; C041348:tcdA protein, Clostridium difficile