Adoptive transfer of mFas ligand into dendritic cells influences the spontaneous resorption rate in the CBA/J x DBA/2 mouse model.
OBJECTIVE
To investigate the effect of mFasL dendritic cells (DC) on the embryo resorption rate in the CBA/J x DBA/2 abortion mouse model.


METHODS
Experimental study.


METHODS
University hospital in a major city in China.


METHODS
101 CBA/J mice and 50 DBA/2 mice.


METHODS
We constructed the eukaryotic expression vector pcDNA3.1-mFasL, derived the DCs from bone marrow of DBA/2 mice, and transfected the DCs with pcDNA3.1-mFasL. The abortion mouse model were established by mating female CBA/J mice with DBA/2 mice. Via the CBA/J x DBA/2 abortion mouse model, five groups were established: group 1: abortion model without treatment; group 2: abortion mouse model injected with DC culture medium (DCCM); group 3: abortion mouse model injected with DC; group 4: abortion mouse model injected with empty plasmid pcDNA3.1-DC; group 5: abortion model mouse injected with pcDNA3.1-FasL-DC.


METHODS
Comparison of difference in the embryo resorption rates of the CBA/J x DBA/2 abortion mouse model treated with either pcDNA3.1-mFasL-DC or different controls observed on gestation days 12 to 14.


RESULTS
The embryo resorption rate was statistically significantly decreased in group 3 treated with DC and group 4 with empty plasmid pcDNA3.1-DC when they compared with group 1 (no treatment for abortion mouse model) and group 2 (injected with DC culture medium, DCCM). Furthermore, abortion model group 5 (injected with pcDNA3.1-mFasL-DC) showed a statistically significantly decreased in embryo resorption rate when compared with the other four groups, including groups 1 and 2 and groups 3 and group 4.


CONCLUSIONS
Adoptive transfer of mFasL-DC can statistically significantly reduce the embryo resorption rate in the CBA/J x DBA/2 abortion mouse model.
Zhao
Aimin
A
Department of Obstetrics and Gynecology, Renji Hospital, School of Medicine, Shanghai JiaoTong University, Shanghai, People's Republic of China.
Xiong
Miao
M
Zhang
Yu
Y
Bao
Shimin
S
Zhang
Jian
J
Qiu
Lihua
L
Lin
Qide
Q
D000026:Abortion, Habitual / Q000235:genetics / Q000276:immunology / Q000628:therapy*; D019264:Adoptive Transfer; D000818:Animals; D001483:Base Sequence; D002478:Cells, Cultured; D003433:Crosses, Genetic; D003713:Dendritic Cells / Q000276:immunology*; D004195:Disease Models, Animal; D020964:Embryo Loss / Q000235:genetics / Q000276:immunology / Q000517:prevention & control*; D053222:Fas Ligand Protein / Q000096:biosynthesis* / Q000235:genetics; D005260:Female; D015316:Genetic Therapy / Q000379:methods*; D007108:Immune Tolerance; D051379:Mice; D008808:Mice, Inbred CBA; D008811:Mice, Inbred DBA; D008969:Molecular Sequence Data; D011247:Pregnancy; D014162:Transfection
D053222:Fas Ligand Protein