[Transfecing hTRT into normal human oral keratinocytes by liposome].
OBJECTIVE
To introduce human telomerase reverse transcriptase (hTRT) into normal human oral keratinocytes(OKC) to expand their life-span.


METHODS
The pEGFP-hTRT was transferred into OKCs by lipofecAMINE2000 and transferring(TF). Expression of hTERT in transfectants was examined by fluorescence microscopy, laser confocal microscopy. Transfection efficiency was examined by flow cytometer. Exogenous hTERT gene was detected by RT-PCR. The transfeced cells were selected by G418 for stable expression.


RESULTS
OKCs were transferred with pEGFP-hTRT by lipofecAMINE2000 and TF. The transfection efficiency was 15.34%. Exogenous hTERT gene could be detected by RT-PCR. The transfeced cells exhibited transient expression of exogenous hTERT gene.


CONCLUSIONS
The recombinant vector pEGFP-hTRT can be transfected into OKC by liposome. TF can improve transfection efficiency. Transient expression of exogenous hTERT is possible in oral keratinocytes.
Department of Oral Medicine, Ninth People's Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine, Shanghai Key Laboratory of Stomatology, Shanghai 200011, China. haiwen39@hotmail.com
Hai-Wen
HW
Zhou
Wen-Jun
WJ
Yang
Ke-Ming
KM
Wang
Zeng-Tong
ZT
Zhou
D005822:Genetic Vectors; D006801:Humans; D015603:Keratinocytes; D008081:Liposomes; D019098:Telomerase; D014162:Transfection
D008081:Liposomes; C509186:TERT protein, human; D019098:Telomerase