Ultraviolet light-induced oxidative stress: effects on antioxidant response of Helicoverpa armigera adults.
Ultraviolet (UV) light (blacklight), which emits UV in the range of 320-400 nm, has been used worldwide in light trapping of insect pests. In this article, we test the hypothesis that one of the effects of UV light irradiation is to increase oxidative stress on insects. The effects of UV light irradiation on total antioxidant capacity, malondialdehyde (MDA) and protein carbonyl contents and the activities of superoxide dismutase (SOD), catalase (CAT), peroxidases (POX) and glutathione-S-transferase (GST)were investigated in Helicoverpa armigera adults. The adults were exposed to UV light for various time periods (0, 30, 60 and 90 min). We found that exposure to UV light for 30 min resulted in increased total antioxidant capacity, protein carbonyl content and activities of SOD, CAT, POX and GST. When the exposure time lasted for 60 and 90 min, the protein carbonyl content and activities of CAT and GST remained significantly higher than the control. However, the antioxidant capacity and SOD activity returned to control levels, and POX activity decreased at 60 and 90 min. Our results confirm the hypothesis that UV light irradiation increases the level of oxidative stress in H. armigera adults.
Meng
Jian-Yu
JY
Key Laboratory of Insect Resources Utilization & Sustainable Pest Management of Hubei Province, Institute of Insect Resources, Huazhong Agricultural University, Wuhan, China.
Zhang
Chang-Yu
CY
Zhu
Fen
F
Wang
Xiao-Ping
XP
Lei
Chao-Liang
CL
D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D019476:Insect Proteins / Q000378:metabolism; D009036:Moths / Q000201:enzymology / Q000378:metabolism* / Q000528:radiation effects*; D018384:Oxidative Stress / Q000528:radiation effects*; D014466:Ultraviolet Rays
D000975:Antioxidants; D019476:Insect Proteins