Comparison of the cytotoxicity of 4 preparations of anti-T-cell globulins in various hematological malignancies.
OBJECTIVE
The cytotoxic effects of 4 ATG preparations (Thymoglobulin, ATG-Fresenius, Lymphoglobulin and ATGAM) in hematological malignancies were compared.


METHODS
Myeloma, myeloid leukaemia and lymphoma cell lines as well as primary CLL and T-cell samples were used. Cells were incubated at 1x10 (6)/mL with 50-500 microg/mL of various ATG preparations with or without complement. Cell viability was analyzed by flow cytometry.


RESULTS
All ATG preparations had potent and similar cytotoxic activity against T-cells, primary CLL cells, NHL cell lines and myeloma cell lines. Resistance of U266 myeloma cells to ATG-induced apoptosis is shared by all 4 ATGs and can be overcome by addition of complement.


CONCLUSIONS
All 4 ATGs have similar cytotoxic activity against hematological malignancies in vitro. If ATGs are used to target hematological malignancies, the choice of which ATG to use would depend on whether the required ATG concentration can be achieved in vivo.
Ayuk
Francis
F
Department of Bone Marrow Transplantation, University Medical Center Hamburg-Eppendorf, D-20246 Hamburg, Germany. ayuketan@uke.uni-hamburg.de
Maywald
Nadja
N
Hannemann
Sandra
S
Larsen
Ulrike
U
Zander
Axel
A
Kröger
Nicolaus
N
D000911:Antibodies, Monoclonal / Q000494:pharmacology*; D000961:Antilymphocyte Serum / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects / Q000276:immunology; D003602:Cytotoxicity, Immunologic; D005434:Flow Cytometry; D019337:Hematologic Neoplasms / Q000188:drug therapy / Q000276:immunology / Q000628:therapy*; D006801:Humans; D007136:Immunoglobulins / Q000494:pharmacology*; D013601:T-Lymphocytes / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000961:Antilymphocyte Serum; D007136:Immunoglobulins; C515141:lymphoglobuline; C512542:thymoglobulin