Sequential changes in serum cytokines reflect viral RNA kinetics in target organs of a coxsackievirus B infection in mice.
BACKGROUND
The pattern of cytokine responses related to viral replication during the course of the common human coxsackievirus B3 (CVB3) infection is not known.


METHODS
Serum levels of 21 cytokines and chemokines were studied (Luminex technique) in CVB3-infected in mice on days 3, 6, and 9 post-infection (p.i.). CVB3 was measured quantitatively (reverse transcriptase polymerase chain reaction) in the liver and pancreas.


RESULTS
Virus levels peaked on day 3 in both the liver and pancreas, but were 1,000-fold higher in the pancreas. IL-17alpha, IFN-gamma, KC, MCP-1, MIP1beta, and RANTES were detected on all days. On day 3 p.i., IL-6, IL-12(p40), KC, MCP-1, RANTES, and TNF-alpha were found to peak. On day 6 p.i., IL-1beta, IL-9, IL-12(p70), IL-13, IL-17alpha, and IFN-gamma peaked. On day 9 p.i., MIP1beta, IL-1beta, MCP-1, and TNF-alpha were still increased. These changes in cytokines may be used to monitor the progress of enteroviral infections in clinical settings.
Lundgren
Magnus
M
Toxicology Division, National Food Administration, 752 27 Uppsala, Sweden. magnus.lundgren@slv.se
Darnerud
Per Ola
PO
Blomberg
Jonas
J
Friman
Göran
G
Ilbäck
Nils-Gunnar
NG
D000818:Animals; D003384:Coxsackievirus Infections / Q000097:blood / Q000175:diagnosis / Q000276:immunology* / Q000503:physiopathology / Q000821:virology; D016207:Cytokines / Q000097:blood*; D018450:Disease Progression; D029822:Enterovirus B, Human / Q000472:pathogenicity / Q000502:physiology*; D005260:Female; D007109:Immunity; D008099:Liver / Q000276:immunology / Q000473:pathology / Q000821:virology*; D051379:Mice; D008807:Mice, Inbred BALB C; D010179:Pancreas / Q000276:immunology / Q000473:pathology / Q000821:virology*; D012367:RNA, Viral / Q000032:analysis; D019562:Viral Load; D014774:Virulence; D014779:Virus Replication
D016207:Cytokines; D012367:RNA, Viral