Identification of natural MHC class II presented phosphopeptides and tumor-derived MHC class I phospholigands.
MHC molecules present protein-derived peptides to T lymphocytes. By developing TiO2-based microcentrifugation columns, we identified the first phosphorylated MHC class I ligands from tumor tissue (renal cell carcinoma) and, by comparison to healthy renal tissue, found one Brf1-derived ligand as potentially tumor-associated. We further discovered the first natural phosphorylated MHC class II ligands. They revealed several novel phosphorylation sites of significant transmembrane receptors, such as Frizzled 6, CXCR4 and CD20.
Department of Immunology, Institute for Cell Biology, University of Tübingen, D-72076 Tübingen, Germany.
Verena S
VS
Meyer
Oliver
O
Drews
Marc
M
Günder
Jörg
J
Hennenlotter
Hans-Georg
HG
Rammensee
Stefan
S
Stevanovic
D018951:Antigens, CD20 / Q000096:biosynthesis; D002292:Carcinoma, Renal Cell / Q000378:metabolism; D045744:Cell Line, Tumor; D051157:Frizzled Receptors / Q000096:biosynthesis; D005805:Genes, MHC Class I; D005802:Genes, MHC Class II; D015395:Histocompatibility Antigens Class I / Q000737:chemistry*; D000949:Histocompatibility Antigens Class II / Q000737:chemistry*; D006801:Humans; D008024:Ligands; D008113:Liver Neoplasms / Q000378:metabolism; D010455:Peptides / Q000737:chemistry*; D010748:Phosphopeptides / Q000737:chemistry*; D010766:Phosphorylation; D019718:Receptors, CXCR4 / Q000096:biosynthesis; D043562:Receptors, G-Protein-Coupled / Q000096:biosynthesis; D014025:Titanium / Q000737:chemistry
D018951:Antigens, CD20; C484687:FZD6 protein, human; D051157:Frizzled Receptors; D015395:Histocompatibility Antigens Class I; D000949:Histocompatibility Antigens Class II; D008024:Ligands; D010455:Peptides; D010748:Phosphopeptides; D019718:Receptors, CXCR4; D043562:Receptors, G-Protein-Coupled; C009495:titanium dioxide; D014025:Titanium