A review of the use of genetically engineered enzymes in electrochemical biosensors.
This article gives an overview of the electrochemical biosensors that incorporate genetically modified enzymes. Firstly, the improvements on the sensitivity and selectivity of biosensors that integrate mutated enzymes are summarised. Next, new trends focused on the oriented immobilisation of mutated enzymes through specific functional groups located at their surface are reviewed. Finally, the effect of enzyme mutations on the electron transfer distance and kinetics of electrochemical biosensors is described.
IRTA, Ctra. Poble Nou, km. 5.5, 43540 Sant Carles de la Ràpita, Tarragona, Spain. monica.campas@irta.cat
Mònica
M
Campàs
Beatriz
B
Prieto-Simón
Jean-Louis
JL
Marty
D000818:Animals; D015374:Biosensing Techniques / Q000379:methods*; D055664:Electrochemical Techniques / Q000379:methods*; D004583:Electrons; D004798:Enzymes / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D015202:Protein Engineering; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism
D004798:Enzymes; D011994:Recombinant Proteins