Transient co-expression for fast and high-yield production of antibodies with human-like N-glycans in plants.
Plant-based transient expression is potentially the most rapid and cost-efficient system for the production of recombinant pharmaceutical proteins, but safety concerns associated with plant-specific N-glycosylation have hampered its adoption as a commercial production system. In this article, we describe an approach based on the simultaneous transient co-expression of an antibody, a suppressor of silencing and a chimaeric human beta1,4-galactosyltransferase targeted for optimal activity to the early secretory pathway in agroinfiltrated Nicotiana benthamiana leaves. This strategy allows fast and high-yield production of antibodies with human-like N-glycans and, more generally, provides solutions to many critical problems posed by the large-scale production of therapeutic and vaccinal proteins, specifically yield, volume and quality.
Vézina
Louis-P
LP
Medicago Inc, Sainte-Foy, QC, Canada. vezinalp@medicago.com
Faye
Loïc
L
Lerouge
Patrice
P
D'Aoust
Marc-André
MA
Marquet-Blouin
Estelle
E
Burel
Carole
C
Lavoie
Pierre-Olivier
PO
Bardor
Muriel
M
Gomord
Véronique
V
D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000302:isolation & purification; D018506:Gene Expression Regulation, Plant; D006031:Glycosylation; D006801:Humans; D007074:Immunoglobulin G / Q000096:biosynthesis / Q000302:isolation & purification; D009237:N-Acetyllactosamine Synthase / Q000235:genetics / Q000378:metabolism; D018515:Plant Leaves / Q000235:genetics / Q000378:metabolism; D030821:Plants, Genetically Modified / Q000235:genetics / Q000378:metabolism; D011134:Polysaccharides / Q000378:metabolism*; D015202:Protein Engineering / Q000379:methods*; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000302:isolation & purification; D014026:Nicotiana / Q000235:genetics / Q000378:metabolism*
D000911:Antibodies, Monoclonal; D007074:Immunoglobulin G; D011134:Polysaccharides; D011993:Recombinant Fusion Proteins; D009237:N-Acetyllactosamine Synthase