Patterns of variation of the major histocompatibility complex class IIB loci in Chinese goose (Anser Cygnoides).
In order to understand the variations of genomic organization of the major histocompatibility complex (MHC) and provide data for the studies on disease resistance of avian species, the MHC class II polymorphism in Chinese Z-goose was investigated for the first time in the present study. Eight alleles, which probably came from different loci, were found in six different geese with only one obvious band in the restriction fragment length polymorphism data. The numbers of nonsynonymous substitutions (dN) in peptide binding region of exon 2 were higher than that of synonymous substitutions (dS), and no stop codons or frameshift mutations were found in this region, indicating that balance selection was in operation, and the sequences are not likely to be pseudogenes. In addition, we successfully obtained five different long MHC class II fragments (about 1,162 bp) in six geese and found that the length of intron 1 was longer than that in chicken and some other birds, but intron 2 seemed to be intermediate in length. The phylogenetic tree appeared to branch in an order consistent with accepted evolutionary pathway.
College of Animal Science and Veterinary Medicine, and Jilin Provincial Key Laboratory of Animal Embryo Engineering, Jilin University, 5333 Xi'an Avenue, Changchun, Jilin Province 130062, People's Republic of China. xzhou65@vip.sina.com
Xu
X
Zhou
Chunjin
C
Li
Kangle
K
Yi
Hongyan
H
Liang
Lu
L
Chen
Yanling
Y
Sun
Shuling
S
Niu
Wei
W
Wu
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002681:China; D005777:Geese / Q000145:classification / Q000235:genetics* / Q000276:immunology; D014644:Genetic Variation; D000949:Histocompatibility Antigens Class II / Q000145:classification / Q000235:genetics*; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid
D000949:Histocompatibility Antigens Class II