Chemical constituents of Isodon nervosus and their cytotoxicity.
Two new ent-kaurane diterpenoids, 6,20,15alpha-trihydroxy-6,7-seco-1alpha,7-olide-ent-kaur-16-ene (1) and 7beta,12alpha-dihydroxy-6beta,15beta-diacetoxy-7alpha,20-epoxy-ent-kaur-2,16-dien-1-one (2), together with the six known compounds, were isolated from the aerial part of Isodon nervosus. The structures of the new compounds were determined by spectral methods (1D, 2D NMR, and MS). Six compounds were assayed for their cytotoxicity against HL60, SMMC-7721, and HeLa human cell lines. Compounds 5, 7, and 8 showed significant cytotoxicity.
Pharmacy College, Xinxiang Medical University, Xinxiang, China. yanfulin03@xxmu.edu.cn
Fu-Lin
FL
Yan
Lan-Qing
LQ
Guo
Chun-Ming
CM
Wang
Ji-Xia
JX
Zhang
D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D045786:Diterpenes, Kaurane / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D004354:Drug Screening Assays, Antitumor; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D018922:HL-60 Cells; D006367:HeLa Cells; D006801:Humans; D037602:Isodon / Q000737:chemistry*; D015394:Molecular Structure; D019906:Nuclear Magnetic Resonance, Biomolecular; D010946:Plants, Medicinal / Q000737:chemistry*; D013237:Stereoisomerism
C540989:6,20,15alpha-trihydroxy-6,7-seco-1alpha,7-olide-ent-kaur-16-ene; C540990:7beta,12alpha-dihydroxy-6beta,15beta-diacetoxy-7alpha,20-epoxy-ent-kaur-2,16-dien-1-one; D000972:Antineoplastic Agents, Phytogenic; D045786:Diterpenes, Kaurane; D004365:Drugs, Chinese Herbal