Genotypic versus phenotypic methods in the detection of Listeria monocytogenes prosthetic joint infection.
A rare case of a severe prosthetic joint infection in a 71-year-old immunocompetent woman is presented. Listeria monocytogenes was identified in two consecutive samples using broad-range PCR and sequencing, whereas cultivation remained negative for the first sample and streptococci of a non-group A streptococci, non-group B streptococci type were detected for the second one. This report demonstrates that the phenotypic approach may lead to misidentification of L. monocytogenes in a routine clinical setting. Molecular methods of pathogen detection might be useful when a rare and/or unexpected micro-organism is present or the sample is collected during antibiotic treatment.
Molecular Genetics Laboratory, Centre for Cardiovascular Surgery and Transplantation, Brno, Czech Republic.
Barbora
B
Žaloudíková
Department of Traumatology, Faculty of Medicine, Masaryk University, Trauma Hospital of Brno, Brno, Czech Republic.
Martin
M
Kelbl
Department of Traumatology, Faculty of Medicine, Masaryk University, Trauma Hospital of Brno, Brno, Czech Republic.
Libor
L
Paša
Institute of Clinical Immunology and Allergology, Faculty of Medicine, Masaryk University, St Anne's University Hospital, Brno, Czech Republic.|Molecular Genetics Laboratory, Centre for Cardiovascular Surgery and Transplantation, Brno, Czech Republic.
Tomáš
T
Freiberger
D000368:Aged; D003470:Culture Media; D004269:DNA, Bacterial / Q000032:analysis; D005260:Female; D005838:Genotype; D006801:Humans; D007720:Knee Prosthesis / Q000382:microbiology*; D008089:Listeria monocytogenes / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D008088:Listeriosis / Q000175:diagnosis* / Q000382:microbiology; D010641:Phenotype; D016133:Polymerase Chain Reaction; D016459:Prosthesis-Related Infections / Q000175:diagnosis* / Q000382:microbiology; D017422:Sequence Analysis, DNA
D003470:Culture Media; D004269:DNA, Bacterial