Ethanol production from rice straw using optimized aqueous-ammonia soaking pretreatment and simultaneous saccharification and fermentation processes.
Rice straw was pretreated using aqueous-ammonia solution at moderate temperatures to enable production of the maximum amount of fermentable sugars from enzymatic hydrolysis. The effects of various operating variables including pretreatment temperature, pretreatment time, the concentration of ammonia and the solid-to-liquid ratio on the degree of lignin removal and the enzymatic digestibility were optimized using response surface methodology. The optimal reaction conditions, which resulted in an enzymatic digestibility of 71.1%, were found to be 69 degrees C, 10h and an ammonia concentration of 21% (w/w). The effects of different commercial cellulases and the additional effect of a non-cellulolytic enzyme, xylanase, were also evaluated. Additionally, simultaneous saccharification and fermentation was conducted with rice straw to assess the ethanol production yield and productivity.
School of Life Sciences and Biotechnology, Korea University, Seoul, Republic of Korea.
Ja Kyong
JK
Ko
Jin Seop
JS
Bak
Min Woo
MW
Jung
Hee Jin
HJ
Lee
In-Geol
IG
Choi
Tae Hyun
TH
Kim
Kyoung Heon
KH
Kim
D000641:Ammonia / Q000737:chemistry*; D001709:Biotechnology / Q000379:methods*; D050260:Carbohydrate Metabolism; D043364:Endo-1,4-beta Xylanases / Q000378:metabolism; D000431:Ethanol / Q000378:metabolism*; D005285:Fermentation; D006868:Hydrolysis; D012275:Oryza / Q000378:metabolism* / Q000648:ultrastructure; D013499:Surface Properties; D013696:Temperature; D013997:Time Factors
D000431:Ethanol; D000641:Ammonia; D043364:Endo-1,4-beta Xylanases