Comparative proteome analysis of two antagonist Bacillus subtilis strains.
Natural wild-type strains of Bacillus subtilis are extensively used in agriculture as biocontrol agents for plants. This study examined two antagonist B. subtilis strains, KB-1111 and KB-1122, and the results illustrated that KB-1122 was a more potent inhibitor of the indicator pathogen than KB- 1111. Thus, to investigate the intrinsic differences between the two antagonist strains under normal culture conditions, samples of KB-1111 and KB-1122 were analyzed using MALDI-TOF-MS. The main differences were related to 20 abundant intracellular and 17 extracellular proteins. When searching the NCBI database, a number of the differentially expressed proteins were identified, including 11 cellular proteins and 10 secretory proteins. Among these proteins, class III stress-response-related ATPase, aconitate hydratase, alpha-amylase precursor, and a secretory protein, endo-1, 4-beta-glucanase, were differentially expressed by the two strains. These results are useful to comprehend the intrinsic differences between the antagonism of KB-1111 and KB-1122.
Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China.
C X
CX
Zhang
X
X
Zhao
F
F
Han
M F
MF
Yang
H
H
Chen
T
T
Chida
S H
SH
Shen
D000898:Antibiosis; D000935:Antifungal Agents / Q000032:analysis* / Q000494:pharmacology; D001412:Bacillus subtilis / Q000502:physiology*; D001426:Bacterial Proteins / Q000032:analysis* / Q000378:metabolism / Q000494:pharmacology; D030562:Databases, Protein; D015180:Electrophoresis, Gel, Two-Dimensional; D020082:Magnaporthe / Q000187:drug effects / Q000502:physiology; D020543:Proteome / Q000032:analysis*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D000935:Antifungal Agents; D001426:Bacterial Proteins; D020543:Proteome