Light-scattering spectroscopy differentiates fetal from adult nucleated red blood cells: may lead to noninvasive prenatal diagnosis.
Present techniques for prenatal diagnosis are invasive and present significant risks of fetal loss. Noninvasive prenatal diagnosis utilizing fetal nucleated red blood cells (fNRBC) circulating in maternal peripheral blood has received attention, since it poses no risk to the fetus. However, because of the failure to find broadly applicable identifiers that can differentiate fetal from adult NRBC, reliable detection of viable fNRBC in amounts sufficient for clinical use remains a challenge. In this Letter we show that fNRBC light-scattering spectroscopic signatures may lead to a clinically useful method of minimally invasive prenatal genetic testing.
Lim
Kee-Hak
KH
Biomedical Imaging and Spectroscopy Laboratory, Department of ObGyn and Reproductive Biology, Beth Israel Deaconess Medical Center, Harvard University, Boston, Massachusetts 02215, USA.
Salahuddin
Saira
S
Qiu
Le
L
Fang
Hui
H
Vitkin
Edward
E
Ghiran
Ionita C
IC
Modell
Mark D
MD
Takoudes
Tamara
T
Itzkan
Irving
I
Hanlon
Eugene B
EB
Sachs
Benjamin P
BP
Perelman
Lev T
LT
D000328:Adult; D004900:Erythroblasts / Q000166:cytology; D004912:Erythrocytes / Q000166:cytology*; D005260:Female; D005333:Fetus / Q000166:cytology*; D006801:Humans; D008027:Light; D011247:Pregnancy; D011296:Prenatal Diagnosis / Q000379:methods*; D012542:Scattering, Radiation; D013057:Spectrum Analysis / Q000379:methods*