Myosin motor-like domain of the class VI chitin synthase CsmB is essential to its functions in Aspergillus nidulans.
Chitin is one of the major cell wall components of ascomycete filamentous fungi, and chitin synthesis plays important roles in the morphogenesis of hyphae. In the Aspergillus nidulans genome, there are two genes, csmA and csmB, that encode a myosin motor-like domain (MMD) at their N-termini and a chitin synthase domain (CSD) at their C-termini. In our previous studies, we found that the MMD of CsmA was required for its functionality, and that CsmA and CsmB had certain overlapping functions essential for polarized filamentous growth. In this study, we constructed a strain that produced CsmB lacking the MMD (CSDeltaMB). This strain exhibited defects similar to those of the csmB deletion mutant. FLAG-tagged CSDeltaMB (CSDeltaMB-FLAG) did not properly localize to the hyphae. CsmB was co-immunoprecipitated with actin in vivo, whereas CSDeltaMB was not. These results suggest that the MMD of CsmB is crucial for its proper localization via interaction with actin.
Tsuizaki
Makusu
M
Department of Biotechnology, The University of Tokyo, Tokyo, Japan.
Takeshita
Norio
N
Ohta
Akinori
A
Horiuchi
Hiroyuki
H
D000199:Actins / Q000378:metabolism; D001233:Aspergillus nidulans / Q000201:enzymology*; D002687:Chitin Synthase / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D025301:Hyphae / Q000378:metabolism; D009154:Mutation; D009218:Myosins / Q000737:chemistry*; D010641:Phenotype; D017434:Protein Structure, Tertiary; D021381:Protein Transport; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism
D000199:Actins; D011993:Recombinant Fusion Proteins; D002687:Chitin Synthase; D009218:Myosins