Cellular behavior change of macrophage after exposure to nanoparticles.
Magnetic nanoparticles (MNPs) are few of the nanoparticles used clinically. When MNPs are delivered into human body, they are ingested by macrophages. We evaluated the cellular response of macrophage after MNPs loading. In face of stimulation by lipopolysaccharide, a strong stimulant derived from bacterial cell wall, MNPs loaded macrophage exhibited decreased phagocytic activity and decreased generation of cytokines such as TNF-alpha, IL-1beta whereas increased nitric oxide generation was noticed. Although these changes might decrease bactiericidal activity, it also alleviates the risk of senses, a life threatening phenomenon in infection patients. The finding has significant implications on nanoparticle based targeted drug delivery.
Hsiao
J K
JK
Institute of Biomedical Engineering, National Taiwan University, Taipei, 10617, Taiwan.
Weng
T I
TI
Tai
M F
MF
Chen
Y F
YF
Wang
Y H
YH
Yang
C Y
CY
Wang
J L
JL
Liu
H M
HM
D000818:Animals; D002460:Cell Line; D053583:Interleukin-1beta / Q000096:biosynthesis; D008264:Macrophages / Q000166:cytology*; D051379:Mice; D053758:Nanoparticles; D009569:Nitric Oxide / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis
D053583:Interleukin-1beta; D014409:Tumor Necrosis Factor-alpha; D009569:Nitric Oxide