Vesicle sizing: Number distributions by dynamic light scattering.
A procedure is described which optimizes nonnegative least squares and exponential sampling fitting methods for analysis of dynamic light scattering (DLS) data from aqueous suspensions of vesicle/liposome systems. This approach utilizes a Rayleigh-Gans-Debye form factor for a coated sphere and yields number distributions which can be compared directly to distributions obtained by freeze-fracture electron microscopy (EM). Excellent agreement between the DLS and EM results are obtained for vesicle size distributions in the 100-200-nm range.
Guelph-Waterloo Program for Graduate Work in Physics, Department of Physics, University of Guelph, Guelph, Ontario N1G 2W1.
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Hallett
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Watton
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Krygsman