Rapid gynogenetic mapping of Xenopus tropicalis mutations to chromosomes.
Pilot forward genetic screens in Xenopus tropicalis have isolated over 60 recessive mutations. Here we present a simple method for mapping mutations to chromosomes using gynogenesis and centromeric markers. When coupled with available genomic resources, gross mapping facilitates evaluation of candidate genes as well as higher resolution linkage studies. Using gynogenesis, we have mapped the genetic locations of the 10 X. tropicalis centromeres, and performed fluorescence in situ hybridization to validate these locations cytologically. We demonstrate the use of this very small set of centromeric markers to map mutations efficiently to specific chromosomes. Developmental Dynamics 238:1398-1406, 2009. (c) 2009 Wiley-Liss, Inc.
Department of Pediatrics and Genetics, Yale University School of Medicine, New Haven, Connecticut, USA.
Mustafa K
MK
Khokha
Vladimir
V
Krylov
Michael J
MJ
Reilly
Joseph G
JG
Gall
Dipankan
D
Bhattacharya
Chung Yan J
CY
Cheung
Sarah
S
Kaufman
Dang Khoa
DK
Lam
Jaroslav
J
Macha
Catherine
C
Ngo
Neha
N
Prakash
Philip
P
Schmidt
Tereza
T
Tlapakova
Toral
T
Trivedi
Lucie
L
Tumova
Anita
A
Abu-Daya
Timothy
T
Geach
Elisenda
E
Vendrell
Holly
H
Ironfield
Ludivine
L
Sinzelle
Amy K
AK
Sater
Dan E
DE
Wells
Richard M
RM
Harland
Lyle B
LB
Zimmerman
D000818:Animals; D002503:Centromere / Q000235:genetics; D002874:Chromosome Mapping / Q000379:methods*; D002875:Chromosomes; D005260:Female; D005819:Genetic Markers; D008297:Male; D008540:Meiosis / Q000502:physiology; D009154:Mutation; D011995:Recombination, Genetic; D014981:Xenopus / Q000235:genetics*
D005819:Genetic Markers