The co-administration of CpG-ODN influenced protective activity of influenza M2e vaccine.
In this report, we investigated the adjuvant effect of CpG-ODN on the immunogenicity and protective efficacy of influenza M2e peptide vaccine. We found that the addition of CpG-ODN 1826 into aluminum-adjuvant M2e peptide vaccine increased M2e-specific Th1 immune response, indicated by higher titers of M2e-specific IgG2a and more IFN-gamma-secreting lymphocytes. However, according to the result from virus challenge, enhancement of M2e-specific Th1 immune response failed to increase the protection against influenza virus. Moreover, when challenged with high dose of influenza virus, the addition of CpG-ODN even weakened the protective activity. These results suggested that the intensity of immune responses was not simply correlated with protective activity of influenza M2e vaccine and more comprehensive criterion should be built up for the evaluation of M2e-based vaccine.
Wu
Fan
F
Laboratory of Immunology, Department of Biology, Tsinghua University, Beijing Key Laboratory for Protein Therapeutics, Beijing 100084, PR China.
Yuan
Xiao-Yi
XY
Li
Jing
J
Chen
Ying-Hua
YH
D000276:Adjuvants, Immunologic / Q000008:administration & dosage / Q000494:pharmacology*; D000534:Alum Compounds / Q000008:administration & dosage / Q000494:pharmacology; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D004247:DNA / Q000008:administration & dosage / Q000494:pharmacology*; D005260:Female; D006801:Humans; D007252:Influenza Vaccines / Q000276:immunology*; D007371:Interferon-gamma / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D009838:Oligodeoxyribonucleotides; D009976:Orthomyxoviridae Infections / Q000276:immunology / Q000517:prevention & control; D016019:Survival Analysis; D018417:Th1 Cells / Q000276:immunology; D022223:Vaccines, Subunit / Q000276:immunology
D000276:Adjuvants, Immunologic; D000534:Alum Compounds; D000914:Antibodies, Viral; C423449:CpG ODN 1826; D007252:Influenza Vaccines; D009838:Oligodeoxyribonucleotides; D022223:Vaccines, Subunit; C041524:aluminum sulfate; D007371:Interferon-gamma; D004247:DNA