Theileria-infected cell line from an African buffalo (Syncerus caffer).
Mononuclear cells were isolated from the peripheral blood of a buffalo infected with a Theileria sp. using density gradient centrifugation, and the cells were put into culture flasks covered by a monolayer of bovine endothelial cells. Twenty days after culture initiation, cells containing macroschizonts were detected in Giemsa-stained smears. The first subculture was carried out on day 45 of culture propagation. Subsequently, infected cells were subcultured twice a week, and each time 1 to 2 x 10(6) per milliliter cells were harvested. DNA was extracted from culture material and a partial polymerase chain reaction amplification of the 18S ribosomal RNA (rRNA) gene was carried out using Theileria genus-specific primers. Sequence data and phylogenetic analysis using the 18S rRNA gene indicated a close relationship to Theileria sp. buffalo, previously described in literature. Here, the first successful attempt to establish a macroschizont-infected lymphoblastoid cell line of Theileria sp. (buffalo) from an African buffalo is described.
Onderstepoort Veterinary Institute, Private Bag X5, Onderstepoort, Pretoria, 0110, South Africa. ZweygarthE@gmail.com
Erich
E
Zweygarth
Otto
O
Koekemoer
Antoinette I
AI
Josemans
Natasha
N
Rambritch
Ronel
R
Pienaar
John
J
Putterill
Abdalla
A
Latif
Fred T
FT
Potgieter
D000818:Animals; D002020:Buffaloes / Q000469:parasitology*; D002417:Cattle; D002478:Cells, Cultured; D016000:Cluster Analysis; D016054:DNA, Protozoan / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D020459:Genes, rRNA; D007963:Leukocytes, Mononuclear / Q000469:parasitology*; D010802:Phylogeny; D016053:RNA, Protozoan / Q000235:genetics; D012337:RNA, Ribosomal, 18S / Q000235:genetics; D017422:Sequence Analysis, DNA; D016794:Theileria / Q000302:isolation & purification*
D016054:DNA, Protozoan; D004275:DNA, Ribosomal; D016053:RNA, Protozoan; D012337:RNA, Ribosomal, 18S