Separation of natural product using columns packed with Fused-Core particles.
Three HPLC columns packed with 3 microm, sub-2 microm, and 2.7 microm Fused-Core (superficially porous) particles were compared in separation performance using two natural product mixtures containing 15 structurally related components. The Ascentis Express C18 column packed with Fused-Core particles showed an 18% increase in column efficiency (theoretical plates), a 76% increase in plate number per meter, a 65% enhancement in separation speed and a 19% increase in back pressure compared to the Atlantis T3 C18 column packed with 3 microm particles. Column lot-to-lot variability for critical pairs in the natural product mixture was observed with both columns, with the Atlantis T3 column exhibiting a higher degree of variability. The Ascentis Express column was also compared with the Acquity BEH column packed with sub-2 microm particles. Although the peak efficiencies obtained by the Ascentis Express column were only about 74% of those obtained by the Acquity BEH column, the 50% lower back pressure and comparable separation speed allowed high-efficiency and high-speed separation to be performed using conventional HPLC instrumentation.
The Dow Chemical Company, Analytical Sciences, Midland, MI 48667, USA. pyang@dow.com
Peilin
P
Yang
George R
GR
Litwinski
Matthias
M
Pursch
Terry
T
McCabe
Krishna
K
Kuppannan
D001688:Biological Products / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation* / Q000379:methods*; D010316:Particle Size; D016062:Porosity; D013499:Surface Properties; D013997:Time Factors
D001688:Biological Products