The linear assembly of a pure glycoenzyme.
A labor of love: The synthesis of an active pure enzyme (RNase) glycoform by the native chemical ligation of a (glyco)peptide and peptide thioester fragments required numerous painstaking steps: the strategy was optimized, active inteins accessed, redox conditions fine-tuned, and glycoamino acid building blocks synthesized.
Davis
Benjamin G
BG
Department of Chemistry, University of Oxford, Chemistry Research Laboratory, Mansfield Road, Oxford, OX1 3TA, UK. ben.davis@chem.ox.ac.uk
D006020:Glycopeptides / Q000737:chemistry*; D006023:Glycoproteins / Q000138:chemical synthesis* / Q000737:chemistry; D047668:Inteins; D010084:Oxidation-Reduction; D015202:Protein Engineering; D017510:Protein Folding; D012260:Ribonucleases / Q000138:chemical synthesis* / Q000737:chemistry
D006020:Glycopeptides; D006023:Glycoproteins; D012260:Ribonucleases