Multiplex detection of breast cancer biomarkers using plasmonic molecular sentinel nanoprobes.
We have demonstrated for the first time the feasibility of multiplex detection using the surface-enhanced Raman scattering-based molecular sentinel (MS) technology in a homogeneous solution. Two MS nanoprobes tagged with different Raman labels were used to detect the presence of the erbB-2 and ki-67 breast cancer biomarkers. The multiplexing capability of the MS technique was demonstrated by mixing the two MS nanoprobes and tested in the presence of single or multiple DNA targets.
Department of Biomedical Engineering, Duke University, Durham, NC 27708, USA.
Hsin-Neng
HN
Wang
Tuan
T
Vo-Dinh
D014408:Biomarkers, Tumor / Q000235:genetics; D001943:Breast Neoplasms / Q000175:diagnosis*; D019411:Clinical Laboratory Techniques / Q000379:methods; D006801:Humans; D019394:Ki-67 Antigen / Q000235:genetics*; D036103:Nanotechnology / Q000379:methods; D009693:Nucleic Acid Hybridization / Q000379:methods*; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000235:genetics*; D013059:Spectrum Analysis, Raman
D014408:Biomarkers, Tumor; D019394:Ki-67 Antigen; D018719:Erb-b2 Receptor Tyrosine Kinases