Synthesis and characterization of polyamidoamine dendrimer-coated multi-walled carbon nanotubes and their application in gene delivery systems.
With the aim of improving the amount and delivery efficiency of genes taken by carbon nanotubes into human cancer cells, different generations of polyamidoamine dendrimer modified multi-walled carbon nanotubes (dMNTs) were fabricated, and characterized by high-resolution transmission electron microscopy, atomic force microscopy, x-ray photoelectron spectroscopy, Raman spectroscopy, Fourier transform infrared spectroscopy and thermogravimetric analysis, revealing the presence of dendrimer capped on the surface of carbon nanotubes. The dMNTs fully conjugated with FITC-labeled antisense c-myc oligonucleotides (asODN), those resultant asODN-dMNTs composites were incubated with human breast cancer cell line MCF-7 cells and MDA-MB-435 cells, and liver cancer cell line HepG2 cells, and confirmed to enter into tumor cells within 15 min by laser confocal microscopy. These composites inhibited the cell growth in time- and dose-dependent means, and down-regulated the expression of the c-myc gene and C-Myc protein. Compared with the composites of CNT-NH(2)-asODN and dendrimer-asODN, no. 5 generation of dendrimer-modified MNT-asODN composites exhibit maximal transfection efficiencies and inhibition effects on tumor cells. The intracellular gene transport and uptake via dMNTs should be generic for the mammalian cell lines. The dMNTs have potentials in applications such as gene or drug delivery for cancer therapy and molecular imaging.
Department of Bio-Nano-Science and Engineering, National Key Laboratory of Nano/Micro Fabrication Technology, Institute of Micro-Nano Science and Technology, Shanghai JiaoTong University, Shanghai, People's Republic of China.
Bifeng
B
Pan
Daxiang
D
Cui
Ping
P
Xu
Cengiz
C
Ozkan
Gao
G
Feng
Mihri
M
Ozkan
Tuo
T
Huang
Bingfeng
B
Chu
Qing
Q
Li
Rong
R
He
Guohan
G
Hu
D000704:Analysis of Variance; D015153:Blotting, Western; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D050091:Dendrimers; D004305:Dose-Response Relationship, Drug; D015536:Down-Regulation; D015972:Gene Expression Regulation, Neoplastic; D018014:Gene Transfer Techniques; D016259:Genes, myc; D006801:Humans; D018625:Microscopy, Atomic Force; D046529:Microscopy, Electron, Transmission; D008856:Microscopy, Fluorescence; D037742:Nanotubes, Carbon / Q000737:chemistry* / Q000648:ultrastructure; D009369:Neoplasms; D011073:Polyamines / Q000737:chemistry*; D016133:Polymerase Chain Reaction; D013057:Spectrum Analysis
D050091:Dendrimers; D037742:Nanotubes, Carbon; C104700:PAMAM Starburst; D011073:Polyamines