[Purification of recombinant lysostaphin by monoclonal antibody affinity chromatography].
Lysostaphin, a specific endopeptidase enzyme derived from Staphylococcus aureus, is a bactericidal agent against Staphylococcus and difficult to be drug-resistant. This study established the monoclonal antibody affinity chromatography to obtain lysostaphin of high purity for drug-use standard. The purified Lysostaphin was of > 95% purity and its recovery rate more than 90%. Moreover, the affinity column kept its efficiency of purification invariable after more than 30 times repeat. Also, the dye release assay validated that the purified lysostaphin had significant bactericidal activity. This method was simple and of high efficacy for the lysostaphin purification and showed its potency in commercial production.
Huang
Jinjiang
J
Shanghai Hi-tech United Bio-technological Research & Development Co. Ltd, Shanghai 201206, China.
Wu
Hongyu
H
Zhang
Jien
J
Huang
Qingshan
Q
D000911:Antibodies, Monoclonal / Q000276:immunology*; D002846:Chromatography, Affinity / Q000379:methods*; D008248:Lysostaphin / Q000096:biosynthesis / Q000302:isolation & purification*; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000302:isolation & purification*; D013211:Staphylococcus aureus / Q000201:enzymology
D000911:Antibodies, Monoclonal; D011994:Recombinant Proteins; D008248:Lysostaphin