Isolation and proteomic analysis of the halotolerant alga Dunaliella salina flagella using shotgun strategy.
Previous studies have demonstrated that flagella/cilia are critical organelles and play diverse roles of motility, sensory perception and development in many eukaryotic cells. However, there is very little information available about flagella composition in Dunaliella salina, a halotolerant, unicellular biflagellate green alga. In the present study, we used strategy of shotgun proteomics to identify flagella proteins after flagella were released and collected from D. salina. A total of 520 groups of proteins were identified under a stringent filter condition (Xcorr > or =1.9, > or =2.2 and > or =3.75; DeltaCn >/= 0.1). In addition to six kinds of known flagella proteins, the putative flagella proteins of D. salina identified by one or more peptides are abundant in signaling, cell division, metabolism, etc. The findings provide guidance for further studies to elucidate the roles of these proteins in the function and assembly of this organelle in microalgae.
Jia
Yanlong
Y
Institute of Tumor Molecular Surgery, The First Affiliated Hospital, Zhengzhou University, 40 Daxue Road, 450052, Zhengzhou, China.
Xue
Lexun
L
Li
Jie
J
Liu
Hongtao
H
D000064:Acclimatization / Q000187:drug effects; D020418:Algal Proteins / Q000032:analysis* / Q000302:isolation & purification*; D000460:Chlorophyta / Q000737:chemistry / Q000378:metabolism*; D030562:Databases, Protein; D005407:Flagella / Q000737:chemistry* / Q000378:metabolism; D006219:Halogens / Q000494:pharmacology / Q000633:toxicity; D020543:Proteome / Q000032:analysis / Q000302:isolation & purification; D040901:Proteomics / Q000379:methods; D055049:Salt Tolerance / Q000502:physiology
D020418:Algal Proteins; D006219:Halogens; D020543:Proteome