Product-specific sample clean-up and HPLC analysis of aflatoxins for a dietary product.
BACKGROUND
A sample clean-up procedure has been developed for a complex dietary ingredient, Unigen Inc. (Lacey, WA, USA) product A (a proprietary mixture of Scutellaria baicalensis and Acacia catechu extracts), for the determination of aflatoxins by HPLC.


OBJECTIVE
To develop an appropriate sample specific clean-up procedure that removes interferences from the sample for the determination of total aflatoxins B(1), B(2), G(1) and G(2) at or lower than 20 ppb by HPLC.


METHODS
The sample specific clean-up procedure was developed based on the modification of AOAC method 990.33.


RESULTS
Coextract interferences were removed with the clean-up procedure. The recovery of total aflatoxins was 70% with RSD about 2%; the LOD and LOQ were 1.6 and 5.2 ppb, respectively, for total aflatoxins; the linearity R(2) > 0.99. None of the samples tested showed the presence of detectable aflatoxins.


CONCLUSIONS
The modified clean-up method was effective to remove coextract interferences from Unigen product A samples.
Zahn
Michael
M
Unigen Inc., 2660 Willamette Drive NE, Lacey, WA 98516, USA.
Jeong
Mijeong Lee
ML
Wang
Desheng
D
Trinh
Thao
T
Fay
Brooke
B
Ma
Wenwen
W
D000045:Acacia / Q000737:chemistry*; D000348:Aflatoxins / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D019587:Dietary Supplements / Q000032:analysis*; D004340:Drug Contamination; D031364:Scutellaria baicalensis / Q000737:chemistry*
D000348:Aflatoxins