Abnormal expression of fibroblast growth factor receptor on lens epithelial cells by dexamethasone: implications for glucocorticoid-induced posterior subcapsular cataract.
We investigated glucocorticoids-induced alteration of bFGF in AqH, FGFR and beta-crystallin in rat lens epithelial cells (LECs). Results. Data showed dexamethasone had no effect on bFGF in AqH, but reduced FGFR1 in LECs. After six months treatment, cells were detected under posterior capsule in 4/12 lenses. In vitro observations revealed a down-regulated FGFR1 or beta-crystallin by dexamethasone. FGFR in LECs may play an important role in formation of glucocorticoid-induced PSC.
Wang
Baichuan
B
Department of Ophthalmology, Daping Hospital, The Third Military Medical University, Chongqing, China. wang_b_c@sina.com.cn
Chen
Chunlin
C
Yin
Xiaolei
X
Chen
Xiaofan
X
Ye
Jian
J
D000818:Animals; D001082:Aqueous Humor / Q000378:metabolism; D015153:Blotting, Western; D002386:Cataract / Q000209:etiology* / Q000473:pathology; D002478:Cells, Cultured; D003907:Dexamethasone / Q000633:toxicity*; D015536:Down-Regulation; D004797:Enzyme-Linked Immunosorbent Assay; D004847:Epithelial Cells / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D016222:Fibroblast Growth Factor 2 / Q000378:metabolism; D005938:Glucocorticoids / Q000633:toxicity*; D007903:Lens Capsule, Crystalline / Q000187:drug effects* / Q000473:pathology; D007908:Lens, Crystalline / Q000187:drug effects* / Q000378:metabolism; D008856:Microscopy, Fluorescence; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D051496:Receptor, Fibroblast Growth Factor, Type 1 / Q000235:genetics / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D038204:beta-Crystallins / Q000235:genetics / Q000378:metabolism
D005938:Glucocorticoids; D012333:RNA, Messenger; D038204:beta-Crystallins; D016222:Fibroblast Growth Factor 2; D003907:Dexamethasone; C496350:Fgfr1 protein, rat; D051496:Receptor, Fibroblast Growth Factor, Type 1