Effects of cadmium telluride quantum dots on cadmium bioaccumulation and metallothionein production to the freshwater mussel, Elliptio complanata.
Nanotechnology has gained increasing commercial attention over recent years and its use has raised concerns about its potential release in the environment. The purpose of this study was to determine the size distribution of CdTe in freshwater, bioavailability and potential toxic effects of cadmium telluride quantum dots (CdTe QD) to the freshwater mussel Elliptio complanata. Mussels were exposed to increasing concentrations (0 to 8 mg Cd L(-1)) of CdTe and 0.5 mg/L CdSO4 for 24 h at 15 degrees C to examine the initial uptake and toxic effects of Cd from CdTe QDs and dissolved CdSO4. After the exposure period, Cd bioaccumulation in the gills, digestive gland and gonad tissues and metallothionein (MT) levels were determined. The results revealed that about 80% of Cd was retained by a 450 nm pore filter (aggregates) and that 14% of the Cd was in the dissolved phase (i.e., eluted through a 1 kDa ultrafiltration membrane) which suggested that uncoated CdTe QDs were not stable in freshwater. In mussels, Cd was accumulated principally by the gills and digestive gland and the bioaccumulation factors of Cd from CdTe were similar to that of dissolved Cd. Indeed, tissue-levels of Cd were below the proportion of dissolved Cd from CdTe which suggests that Cd rather comes from the dissociation of Cd from the ingested QDs than from the internalization of the QDs in mussel tissues. The levels of MT were induced in both the digestive gland and gonad but were readily decreased in the gills by both CdTe and CdSO4. The observed decrease in the metallic form of MT might result from the oxidative stress by CdTe and dissolved Cd. In conclusion, uncoated CdTe QD in freshwater leads to aggregates and a dissolved component of Cd where the latter explained the contribution of the observed accumulation pattern in mussel tissues and effects on MT levels in mussels.
Peyrot
Caroline
C
Department of Chemistry, Université de Montréal, PO Box 6128 Downtown, Montreal, QC, Canada H3C 3J7.
Gagnon
Christian
C
Gagné
François
F
Willkinson
Kevin J
KJ
Turcotte
Patrice
P
Sauvé
Sébastien
S
D000818:Animals; D001682:Biological Availability; D049872:Bivalvia / Q000187:drug effects* / Q000378:metabolism; D001822:Body Burden; D019187:Cadmium Compounds / Q000378:metabolism / Q000633:toxicity*; D004064:Digestive System / Q000187:drug effects / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D005618:Fresh Water; D005880:Gills / Q000187:drug effects / Q000378:metabolism; D006066:Gonads / Q000187:drug effects / Q000378:metabolism; D008668:Metallothionein / Q000378:metabolism*; D018384:Oxidative Stress; D045663:Quantum Dots; D012995:Solubility; D013431:Sulfates / Q000378:metabolism / Q000633:toxicity*; D013691:Tellurium / Q000378:metabolism / Q000633:toxicity*; D015854:Up-Regulation; D014874:Water Pollutants, Chemical / Q000633:toxicity*
D019187:Cadmium Compounds; D013431:Sulfates; D014874:Water Pollutants, Chemical; D008668:Metallothionein; C037123:cadmium sulfate; D013691:Tellurium; C028337:cadmium telluride