Dominant negative autoregulation limits steady-state repression levels in gene networks.
Many transcription factors repress transcription of their own genes. Negative autoregulation has been shown to reduce cell-cell variation in regulatory protein levels and speed up the response time in gene networks. In this work we examined transcription regulation of the galS gene and the function of its product, the GalS protein. We observed a unique operator preference of the GalS protein characterized by dominant negative autoregulation. We show that this pattern of regulation limits the repression level of the target genes in steady states. We suggest that transcription factors with dominant negative autoregulation are designed for regulating gene expression during environmental transitions.
Semsey
Szabolcs
S
Department of Genetics, Eötvös Lóránd University, Budapest, Hungary. semseys@yahoo.com
Krishna
Sandeep
S
Erdossy
János
J
Horváth
Péter
P
Orosz
László
L
Sneppen
Kim
K
Adhya
Sankar
S
D004926:Escherichia coli / Q000502:physiology*; D029968:Escherichia coli Proteins / Q000096:biosynthesis* / Q000235:genetics*; D005786:Gene Expression Regulation; D053263:Gene Regulatory Networks; D006706:Homeostasis; D012097:Repressor Proteins / Q000096:biosynthesis* / Q000235:genetics*
D029968:Escherichia coli Proteins; C050993:Galactose repressor proteins; D012097:Repressor Proteins