Selective activity of Mucor plumbeus reductase towards (-)-camphorquinone.
The biotransformation of 1R-(-)-camphorquinone, achieved by growing cells of four fungi species isolated from soil (Mucor plumbeus, Lecanicillium muscarium, Thamnostylum sp. and Syncephalastrum racemosum), was investigated in optimized culture media for each species. Fungi were grown aerobically under shaking and their activities with respect to camphorquinone were monitored for 20 days by gas chromatography coupled to mass spectrometry (GCMS). Camphorquinone was found to be stable in control flasks throughout the experiment. The most interesting results were found for M. plumbeus, which was only able to perform monoreduction of camphorquinone when cultivated on a glucose-peptone-yeast extract medium. Large-scale experiments were set up and the camphorquinone biotransformation products formed by M. plumbeus were purified by column chromatography and identified by (1)H and (13)C nuclear magnetic resonance (NMR). Theoretical calculations were employed as a complementary technique to unambiguously identify the biotransformation products. These findings suggest that M. plumbeus could be of great use for the selective reduction of camphorquinone and related compounds.
Departamento de Química, ICEx, Universidade Federal de Minas Gerais, Campus Universitário, Belo Horizonte, MG 31270-901, Brazil.
Giovanni Gontijo
GG
de Souza
Cleber Paulo Andrada
CP
Anconi
Sjef
S
Cornelissen
Wagner Batista
WB
De Almeida
Hélio Ferreira
HF
Dos Santos
Isabel Cristina Pereira
IC
Fortes
Jacqueline Aparecida
JA
Takahashi
D000332:Aerobiosis; D001711:Biotransformation; D003470:Culture Media / Q000737:chemistry; D005656:Fungal Proteins / Q000378:metabolism*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D009090:Mucorales / Q000201:enzymology* / Q000302:isolation & purification / Q000378:metabolism; D010088:Oxidoreductases / Q000378:metabolism*; D012988:Soil Microbiology; D013379:Substrate Specificity; D013729:Terpenes / Q000378:metabolism*
D003470:Culture Media; D005656:Fungal Proteins; D013729:Terpenes; C048339:camphoroquinone; D010088:Oxidoreductases