Patterns and dynamics of subventricular zone neuroblast migration in the ischemic striatum of the adult mouse.
The migratory behavior of neuroblasts after a stroke is poorly understood. Using time-lapse microscopy, we imaged migration of neuroblasts and cerebral vessels in living brain slices of adult doublecortin (DCX, a marker of neuroblasts) enhanced green fluorescent protein (eGFP) transgenic mice that were subjected to 7 days of stroke. Our results show that neuroblasts originating in the subventricular zone (SVZ) of adult mouse brain laterally migrated in chains or individually to reach the ischemic striatum. The chains were initially formed at the border between the SVZ and the striatum by neuroblasts in the SVZ and then extended to the striatum. The average speed of DCX-eGFP-expressing cells within chains was 28.67+/-1.04 microm/h, which was significantly faster (P<0.01) than the speed of the cells in the SVZ (17.98+/-0.57 microm/h). Within the ischemic striatum, individual neuroblasts actively extended or retracted their processes, suggestive of probing the immediate microenvironment. The neuroblasts close to cerebral blood vessels exhibited multiple processes. Our data suggest that neuroblasts actively interact with the microenvironment to reach the ischemic striatum by multiple migratory routes.
Department of Neurology, Henry Ford Health Sciences Center, Detroit, Michigan 48202, USA.
Rui L
RL
Zhang
Michael
M
Chopp
Sara R
SR
Gregg
Yier
Y
Toh
Cindi
C
Roberts
Yvonne
Y
Letourneau
Benjamin
B
Buller
Longfei
L
Jia
Siamak
S
P Nejad Davarani
Zheng G
ZG
Zhang
D000818:Animals; D002545:Brain Ischemia / Q000473:pathology*; D002465:Cell Movement; D002552:Cerebral Ventricles / Q000473:pathology; D002560:Cerebrovascular Circulation; D003342:Corpus Striatum / Q000473:pathology*; D000089662:Doublecortin Protein; D007700:Kinetics; D051379:Mice; D018715:Microscopy, Video; D009474:Neurons / Q000166:cytology* / Q000502:physiology; D013234:Stem Cells / Q000166:cytology / Q000502:physiology; D020521:Stroke / Q000473:pathology