Control of Pseudomonas aeruginosa and Stenotrophomonas maltophilia contamination of microfiltered water dispensers with peracetic acid and hydrogen peroxide.
The abilities of peracetic acid and hydrogen peroxide to remove or reduce Pseudomonas aeruginosa and Stenotrophomonas maltophilia in output water from microfiltered water dispensers (MWDs) were investigated. Two MWDs were inoculated with strains of P. aeruginosa and S. maltophilia isolated from water. Dispensers A and B were disinfected with 10% (v/v) peracetic acid (PAA) and 3% (v/v) hydrogen peroxide (HP) respectively. Each dispenser was disinfected three times at monthly intervals with contact times of 10, 30 and 40 min. Water dispensed by the MWDs was collected immediately before and after each treatment and then twice weekly for the remaining period. Once a week a sample of the tap water entering the dispensers was tested. P. aeruginosa and S. maltophilia were enumerated in the 90 samples collected during 6 months. In the output water from the dispensers before the first treatment, the number of the bacteria was 3 to 4 log cfu/100 mL. Treatment with PAA greatly reduced the numbers of P. aeruginosa and S. maltophilia in the dispensed water initially. However, by 2 days after treatment, the numbers increased and remained high. In the case of disinfection with HP for 40 min, P. aeruginosa was not detected in most of the samples (73.7%). Numbers of S. maltophilia decreased with increasing time after treatment.
Sacchetti
Rossella
R
Department of Medicine and Public Health, Division of Hygiene, University of Bologna, Via S. Giacomo, 12-40126 Bologna, Italy. rossella.sacchetti@unibo.it
De Luca
Giovanna
G
Zanetti
Franca
F
D015169:Colony Count, Microbial; D003257:Consumer Product Safety; D004202:Disinfectants / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004326:Drinking; D004866:Equipment Contamination; D005374:Filtration; D005506:Food Contamination / Q000032:analysis / Q000517:prevention & control; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D010463:Peracetic Acid / Q000494:pharmacology*; D011550:Pseudomonas aeruginosa / Q000187:drug effects* / Q000254:growth & development; D020615:Stenotrophomonas maltophilia / Q000187:drug effects* / Q000254:growth & development; D013997:Time Factors; D014871:Water Microbiology; D014881:Water Supply / Q000032:analysis / Q000592:standards*
D004202:Disinfectants; D006861:Hydrogen Peroxide; D010463:Peracetic Acid