SSR and SCAR mapping of a multiple-allele male-sterile gene in Chinese cabbage (Brassica rapa L.).
The genic multiple-allele inherited male-sterile gene Ms in Chinese cabbage (Brassica rapa L.) was identified as a spontaneous mutation. Applying this gene to hybrid seed production, several B. rapa cultivars have been successfully bred in China. A BC(1) population (244 plants) was constructed for mapping the Ms gene. Screening 268 simple sequence repeat (SSR) markers which cover the entire genome of Chinese cabbage was performed with bulked segregant analysis (BSA). On the basis of linkage analysis, the Ms gene was located on linkage group R07. In addition, through the amplified fragment length polymorphism (AFLP) and the sequence-characterized amplified region (SCAR) techniques combining BSA, two SCAR markers which were converted from corresponding AFLP markers flanked the Ms gene. Finally, a genetic map of the Ms gene was constructed covering a total interval of 9.0 cM. Two SCAR markers, syau_scr01 and syau_scr04, flanked the Ms gene at distances of 0.8 and 2.5 cM, respectively. All the SSR markers (cnu_m273, cnu_m030, cnu_m295, and syau_m13) were mapped on the same side of the gene as syau_scr04, the nearest one of which, syau_m13, was mapped at a distance of 3.3 cM. These SSR and SCAR markers may be useful in marker-assisted selection and map-based cloning.
Department of Horticulture, Shenyang Agricultural University, Shenyang, China. fenghuiaaa@263.net
Hui
H
Feng
Peng
P
Wei
Zhong-Yun
ZY
Piao
Zhi-Yong
ZY
Liu
Cheng-Yu
CY
Li
Yu-Gang
YG
Wang
Rui-Qin
RQ
Ji
Shu-Juan
SJ
Ji
Ting
T
Zou
Su-Ryun
SR
Choi
Yong-Pyo
YP
Lim
D000483:Alleles; D054458:Amplified Fragment Length Polymorphism Analysis; D029741:Brassica rapa / Q000235:genetics*; D002874:Chromosome Mapping; D020090:Chromosome Segregation; D017343:Genes, Plant; D008040:Genetic Linkage; D005819:Genetic Markers; D005838:Genotype; D018895:Microsatellite Repeats / Q000235:genetics*; D051479:Plant Infertility / Q000235:genetics*; D011110:Polymorphism, Genetic
D005819:Genetic Markers