Preparation and characterization of fibroin/hyaluronic acid composite scaffold.
Hyaluronic acid (HA) was added into fibroin solution to prepare fibroin-based porous composite scaffolds. HA exhibited important effects on pore formation and hydrophilicity of fibroin-based scaffold. The aqueous-fibroin/HA scaffolds had highly homogeneous and interconnected pores with porosity of above 90% and controllable pore size ranging from 123 to 253 microm. The water take-up of fibroin/HA scaffolds increased significantly with the increase of HA content. Containing HA at a defined content range, such as 3-6%, fibroin-based scaffolds' affinity to primary neural cells was improved. In 6%HA/fibroin scaffolds, neurosphere-forming cell migrated from their original aggregate and adhered tightly to the surface of scaffolds.
Ren
Yong-Juan
YJ
Biomaterials Laboratory, Department of Materials Science and Engineering, Tsinghua University, Beijing 100084, People's Republic of China.
Zhou
Zi-You
ZY
Liu
Bing-Fang
BF
Xu
Qun-Yuan
QY
Cui
Fu-Zhai
FZ
D000818:Animals; D001696:Biomechanical Phenomena; D012831:Bombyx; D002448:Cell Adhesion / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D005260:Female; D005349:Fibroins / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology*; D006820:Hyaluronic Acid / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology*; D057927:Hydrophobic and Hydrophilic Interactions; D019476:Insect Proteins / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D008855:Microscopy, Electron, Scanning; D009474:Neurons / Q000166:cytology / Q000187:drug effects; D016062:Porosity / Q000187:drug effects; D011247:Pregnancy; D051381:Rats; D017207:Rats, Sprague-Dawley; D017550:Spectroscopy, Fourier Transform Infrared; D013234:Stem Cells / Q000166:cytology / Q000187:drug effects; D023822:Tissue Engineering; D054457:Tissue Scaffolds / Q000737:chemistry*; D014867:Water / Q000737:chemistry
D019476:Insect Proteins; D014867:Water; D006820:Hyaluronic Acid; D005349:Fibroins