Critical assessment of an in vitro bovine respiratory organ culture system: a model of bovine herpesvirus-1 infection.
A bovine in vitro organ culture (BIVOC) system was evaluated as a model to study host and pathogen events during the course of bovine herpesvirus-1 infection. Upper respiratory tract epithelium, from slaughtered animals, was cultured in an air-liquid interface system and integrity, viability, and TNF-alpha gene expression of tissue explants were monitored over 72h in the presence or absence of infection by bovine herpesvirus type 1 (BHV-1). Uninfected explants maintained viability and integrity over the 72h time course although histological signs of degeneration were first visible from 24h of culture. Explants were productively infected with BHV-1 and typical, dose dependent, cytopathic changes were observed in response to infection. Regulation of TNF-alpha gene expression in uninfected explants varied over time and was region-specific but there was significant down-regulation of TNF-alpha gene expression at 2h post-infection when compared to uninfected controls at the same time point. Taking caveats into consideration the BIVOC system shows promise as a tool for analysis of immediate or early events in host-pathogen interaction.
Department of Veterinary Medicine, University of Cambridge, Madingley Road, Cambridge, UK.
H S
HS
Niesalla
A
A
Dale
J D
JD
Slater
S F E
SF
Scholes
J
J
Archer
D J
DJ
Maskell
A W
AW
Tucker
D000818:Animals; D002417:Cattle; D002470:Cell Survival; D020869:Gene Expression Profiling; D006566:Herpesviridae Infections / Q000473:pathology* / Q000821:virology*; D007242:Herpesvirus 1, Bovine / Q000254:growth & development*; D009924:Organ Culture Techniques; D020545:Respiratory Mucosa / Q000473:pathology* / Q000821:virology*; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis
D014409:Tumor Necrosis Factor-alpha