[Analysis of demeton-S-methyl, oxydemeton-methyl and demeton-S-methylsulfone in agricultural products by LC-MS].
We studied the simultaneous determination of demeton-S-methyl, oxydemeton-methyl, and their oxide demeton-S-methylsulfone, in agricultural products by liquid chromatography coupled with mass spectrometry (LC-MS). The sample homogenized with antioxidants L-ascorbic acid and butylhydroxytoluene was extracted with acetone. An aliquot of the crude extract was reextracted with ethyl acetate by using an EXtrelut column. After hexane/acetonitrile partitioning lipid-rich samples such as cereals, the extract was cleaned up on a PSA column or tandem graphitized carbon/PSA column, and determined by ESI-SIM mode LC-MS. Average recoveries (n=5) of compounds from ten kinds of samples fortified at the analyte concentration of 0.05 microg/g were from 73.8% to 102.5%, and the relative standard deviations were <or=5.7%.
Aichi Prefectural Institute of Public Health, Kita-ku, Nagoya 4628576, Japan.
Eiji
E
Ueno
Yuka
Y
Kabashima
Harumi
H
Oshima
Tsutomu
T
Ohno
Satoru
S
Nemoto
Tamio
T
Maitani
D002853:Chromatography, Liquid; D018556:Crops, Agricultural / Q000737:chemistry*; D007306:Insecticides / Q000032:analysis* / Q000302:isolation & purification; D013058:Mass Spectrometry; D063086:Organothiophosphates / Q000032:analysis* / Q000302:isolation & purification; D009946:Organothiophosphorus Compounds / Q000032:analysis* / Q000302:isolation & purification
D007306:Insecticides; D063086:Organothiophosphates; D009946:Organothiophosphorus Compounds; C026086:methyl demeton; C030064:demeton-S-methyl