Optimization of renewal regime for improvement of polysaccharides production from Porphyridium cruentum by uniform design.
To probe the effects of renewal regime on the production of polysaccharides, Porphyridium cruentum was cultured semi-continuously in flat plate photobioreactor. Uniform design was used to optimize renewal conditions. Quadratic mathematic models related to productivity, total recovery yield of biomass and polysaccharides were set up to clarify the influence of individual factors and their interactions. According to the mathematic models, the optimal semi-continuous condition for total yield of polysaccharide was NaNO3 3.5 g/L, renewal rate 27%, renewal period 2.91 days. The optimal condition for polysaccharide output rate was NaNO3 0.5 g/L, renewal rate 5%, renewal period 7 days. With the optimal renewal regime, the maximal total recovery yields of polysaccharide achieved at 29.4 g, which was 1.57 times higher than that of batch cultivation. The maximum output rate of polysaccharide was 68.64 mg/L per day, which was 2.02 times higher than previous reported data.
Sun
Liqin
L
Ocean School of Yantai University, 264005 Yantai, Shandong, People's Republic of China.
Wang
Changhai
C
Ma
Cuihua
C
Shi
Lei
L
D018533:Biomass; D019149:Bioreactors; D001709:Biotechnology / Q000379:methods; D003470:Culture Media; D004867:Equipment Design; D006863:Hydrogen-Ion Concentration; D008954:Models, Biological; D015233:Models, Statistical; D008962:Models, Theoretical; D009566:Nitrates / Q000737:chemistry; D011134:Polysaccharides / Q000737:chemistry*; D044665:Porphyridium / Q000378:metabolism*; D013696:Temperature
D003470:Culture Media; D009566:Nitrates; D011134:Polysaccharides; C031618:sodium nitrate