Biogenesis of bacterial membrane vesicles.
Membrane vesicle (MV) release remains undefined, despite its conservation among replicating Gram-negative bacteria both in vitro and in vivo. Proteins identified in Salmonella MVs, derived from the envelope, control MV production via specific defined domains that promote outer membrane protein-peptidoglycan (OM-PG) and OM protein-inner membrane protein (OM-PG-IM) interactions within the envelope structure. Modulation of OM-PG and OM-PG-IM interactions along the cell body and at division septa, respectively, maintains membrane integrity while co-ordinating localized release of MVs with distinct size distribution and protein content. These data support a model of MV biogenesis, wherein bacterial growth and division invoke temporary, localized reductions in the density of OM-PG and OM-PG-IM associations within the envelope structure, thus releasing OM as MVs.
Deatherage
Brooke L
BL
Department of Microbiology, University of Washington, 1959 NE Pacific St., Seattle, WA 98195, USA.
Lara
J Cano
JC
Bergsbaken
Tessa
T
Rassoulian Barrett
Sara L
SL
Lara
Stephanie
S
Cookson
Brad T
BT
D001425:Bacterial Outer Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D010457:Peptidoglycan / Q000235:genetics / Q000378:metabolism*; D012475:Salmonella / Q000166:cytology* / Q000235:genetics / Q000378:metabolism; D022161:Transport Vesicles / Q000235:genetics / Q000378:metabolism*
D001425:Bacterial Outer Membrane Proteins; D010457:Peptidoglycan