Composition analysis and antioxidant activity of polysaccharide from Dendrobium denneanum.
Three polysaccharide fractions (DDP1-1, DDP2-1 and DDP3-1) were successfully purified from the crude polysaccharide of Dendrobium denneanum by DEAE-Cellulose and Sephadex G-200 column chromatography. The average molecular weights (Mws) of these fractions were 51.5, 26.1 and 6.95 kDa, respectively. Monosaccharide components analysis indicated that DDP1-1 and DDP2-1 were composed of arabinose, xylose, mannose, glucose and galactose in a molar ratio of 1.00:2.82:57.11:140.82:7.76 and 1.00:1.62:1.18:77.5:7.79. DDP3-1 was composed of arabinose, mannose, glucose and galactose in a molar ratio of 1.00:1.03:8.84:2.00. On the basis of antioxidant test in vitro, DDP2-1 exhibited the highest antioxidant ability among these samples.
Fan
Yijun
Y
College of Life Sciences, Sichuan University, Chengdu 610064, PR China.
He
Xingjin
X
Zhou
Songdong
S
Luo
Aoxue
A
He
Tao
T
Chun
Ze
Z
D000975:Antioxidants / Q000737:chemistry* / Q000302:isolation & purification*; D052160:Benzothiazoles / Q000737:chemistry; D001713:Biphenyl Compounds / Q000737:chemistry; D031667:Dendrobium / Q000737:chemistry*; D017665:Hydroxyl Radical / Q000737:chemistry; D010851:Picrates / Q000737:chemistry; D011134:Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification*; D017550:Spectroscopy, Fourier Transform Infrared; D013451:Sulfonic Acids / Q000737:chemistry
D000975:Antioxidants; D052160:Benzothiazoles; D001713:Biphenyl Compounds; D010851:Picrates; D011134:Polysaccharides; D013451:Sulfonic Acids; C002502:2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid; D017665:Hydroxyl Radical; C004931:1,1-diphenyl-2-picrylhydrazyl