Expression of four trypsin-like serine proteases from the Chinese shrimp, Fenneropenaeus chinensis, as regulated by pathogenic infection.
Four trypsin-like serine proteases, designated as Fctry1, Fctry2, Fctry3, and Fctry4, were obtained from the hepatopancreas of the Chinese shrimp, Fenneropenaeus chinensis. Three trypsin-like serine proteases, specifically Fctry1, 2, and 3 were observed to have the conserved catalytic triad (H, D, and S). Regarding Fctry4, the catalytic triad S was substituted by F, and the mature peptide was found to be inactive. Further analysis for similarities indicated that Fctry1, 2 and 4 were 89-92% identical to trypsins from Pacific white shrimp (Litopenaeus vannamei); while Fctry3 was only 42% identical to trypsins from salmon louse (Lepeophtheirus salmonis). The genomic organizations of Fctry1, 2 and 4 are also quite different from Fctry3. So, Fctry3 may be a new member of the trypsin-like serine protease family. Moreover, a semi-quantitative reverse transcription polymerase chain reaction (PCR) and quantitative real-time PCR was carried out to analyze the distribution profiles and expression patterns after they were subjected to a bacterial and viral challenge. The results showed that the four trypsin-like serine proteases were upregulated in the hepatopancreas of shrimp infected with the white spot syndrome virus (WSSV), and Fctry3 increased after a bacteria challenge. Therefore, these trypsin-like serine proteases might be involved in the innate defense reactions against different pathogens in Chinese shrimp.
Shi
Xiu-Zhen
XZ
School of Life Sciences, Shandong University, Jinan, Shandong 250100, China.
Ren
Qian
Q
Zhao
Xiao-Fan
XF
Wang
Jin-Xing
JX
D000595:Amino Acid Sequence; D000818:Animals; D003001:Cloning, Molecular; D020869:Gene Expression Profiling; D015971:Gene Expression Regulation, Enzymologic; D054884:Host-Pathogen Interactions; D007527:Isoenzymes / Q000145:classification / Q000235:genetics; D008969:Molecular Sequence Data; D033561:Penaeidae / Q000201:enzymology / Q000235:genetics* / Q000821:virology; D010802:Phylogeny; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D057057:Serine Proteases / Q000145:classification / Q000235:genetics*; D014357:Trypsin / Q000235:genetics; D046848:White spot syndrome virus 1 / Q000502:physiology
D007527:Isoenzymes; D057057:Serine Proteases; D014357:Trypsin