Chitosan-SiO2-multiwall carbon nanotubes nanocomposite: a novel matrix for the immobilization of creatine amidinohydrolase.
A matrix made up of chitosan-SiO(2)-multiwall carbon nanotubes (CHIT-SiO(2)-MWCNTs) nanocomposite was fabricated to investigate the immobilization of creatine amidinohydrolase (CAH). CAH enzyme was covalently immobilized with the CHIT-SiO(2)-MWCNTs matrix using glutaraldehyde as a linker. The resulting CAH/CHIT-SiO(2)-MWCNTs biomatrix was characterized with Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM) and cyclic voltammetry (CV) taking CHIT-SiO(2)-MWNTs as a reference. The influence of various parameters on CAH enzyme activity within the matrix was investigated including pH, temperature, and time. The Michaelis-Menten constant and apparent activities for the CAH enzyme were calculated to be 0.58 mM and 83.16 mg/cm(2), respectively; indicating CHIT-SiO(2)-MWCNTs nanocomposite matrix has a high affinity to immobilize CAH enzyme.
Tiwari
Ashutosh
A
Division of Engineering Materials, National Physical Laboratory, Dr. K.S. Krishnan Marg, New Delhi 110012, India. tiwaria@mail.nplindia.org
Dhakate
Sanjay R
SR
D000188:Actinobacillus / Q000201:enzymology; D048271:Chitosan / Q000737:chemistry*; D004563:Electrochemistry; D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000737:chemistry* / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008855:Microscopy, Electron, Scanning; D053761:Nanocomposites / Q000737:chemistry*; D037742:Nanotubes, Carbon / Q000737:chemistry*; D012822:Silicon Dioxide / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared; D013696:Temperature; D013997:Time Factors; D014512:Ureohydrolases / Q000737:chemistry* / Q000378:metabolism
D004800:Enzymes, Immobilized; D037742:Nanotubes, Carbon; D012822:Silicon Dioxide; D048271:Chitosan; D014512:Ureohydrolases; C019944:creatinase