Transcription of an RNA aptamer by a DNA polymerase.
Therminator DNA polymerase, a variant of the 9 degrees N DNA polymerase, is shown to synthesize a functional RNA aptamer; thus providing a simple route for making DNA-tagged RNA aptamers for use in DNA nanotechnology.
Center for BioOptical Nanotechnology, The Biodesign Institute, Department of Chemistry and Biochemistry, Arizona State University, Tempe, AZ 85287-5201, USA.
Elizabeth O
EO
McCullum
John C
JC
Chaput
D052157:Aptamers, Nucleotide / Q000737:chemistry* / Q000235:genetics*; D001665:Binding Sites; D004247:DNA / Q000737:chemistry* / Q000235:genetics; D017931:DNA Primers / Q000737:chemistry / Q000235:genetics; D004259:DNA-Directed DNA Polymerase / Q000235:genetics*; D036103:Nanotechnology; D014158:Transcription, Genetic
D052157:Aptamers, Nucleotide; D017931:DNA Primers; D004247:DNA; D004259:DNA-Directed DNA Polymerase