Molecular characterisation of two novel double-stranded RNA elements from Phlebiopsis gigantea.
The incomplete sequences of two large, 10-12 kbp, double-stranded RNAs (dsRNAs) found in the TW-2 isolate of the saprophytic fungus, Phlebiopsis gigantea (Pg) are reported. Both PgV-TW2 dsRNA1 and dsRNA2 potentially encode fusion proteins which are apparently expressed by a translational frameshifting mechanism. The C-terminal region of both predicted proteins was 21% identical and contained the eight motifs conserved in RNA-dependent RNA polymerases of dsRNA mycoviruses and had highest similarity with members of the family Totiviridae, but possibly do not form virions. The remainder of the N-terminal protein sequences predicted from the PgV-TW2 dsRNA1 and dsRNA2 sequences and the 3'-terminal nucleotide sequences of both dsRNAs had no homology with one another or any sequence in the database suggesting that individually both may be members of novel families of mycoviruses.
Division of Biology, Imperial College London, Sir Alexander Fleming Building, Imperial College Road, London SW7 2AZ, UK.
Zisis
Z
Kozlakidis
Caroline V
CV
Hacker
Dominic
D
Bradley
Atif
A
Jamal
Xiyu
X
Phoon
Joan
J
Webber
Clive M
CM
Brasier
Kenneth W
KW
Buck
Robert H A
RH
Coutts
D000595:Amino Acid Sequence; D001487:Basidiomycota / Q000821:virology*; D008969:Molecular Sequence Data; D010802:Phylogeny; D012330:RNA, Double-Stranded / Q000235:genetics* / Q000302:isolation & purification*; D012324:RNA-Dependent RNA Polymerase / Q000235:genetics; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D019196:Totiviridae / Q000235:genetics; D014764:Viral Proteins / Q000235:genetics
D012330:RNA, Double-Stranded; D014764:Viral Proteins; D012324:RNA-Dependent RNA Polymerase