Modulators of ion transport in nasal polyps: an in situ measurement of short-circuit current.
OBJECTIVE
To examine possible modulators of the ion transport through the apical membrane of the nasal polyps.


METHODS
The study was conducted using the freshly-excised nasal polyps from the patients with chronic sinusitis. A voltage-sensitive vibrating probe technique was introduced to monitor the short-circuit current across the apical membrane of the polyp at 37°C.


RESULTS
In the presence of amiloride, Adenosine 5'-triphosphate induced 4,4'-Diisothiocyanatostilbene-2,2'-disulfonic acid-sensitive chloride current. Uridine 5'-diphosphate was less potent than Uridine 5'-triphosphate, and adenosine increased chloride secretion, which was blocked by the antagonist, 8-(p-sulfophenyl) theophylline on adenosine receptor. Based on the pharmacologic profiles, multiple purinergic receptors, including P2Y₂, P2Y₆, and P1 receptors, were functionally expressed. However, P2X receptor agonists (α,β-methyleneadenosine 5'-triphosphate and 2'- & 3'-O-[4-benzoyl-benzoyl] adenosine 5'-triphosphate), Cystic fibrosis conductance regulator (CFTR) activator (genistein), nitric oxide substrate (L-arginine), and nitric oxide donor (sodium nitroprusside) had no significant effect on the short circuit current.


CONCLUSIONS
Among tested drugs, P2Y receptor agonists were major modulators of ion transport in nasal polyps in situ.
Department of Otorhinolaryngology, College of Medicine, Seoul National University, Seoul, Korea.
Jun Ho
JH
Lee
Chae-Seo
CS
Rhee
Dae Woo
DW
Kim
Chul Hee
CH
Lee