Magnetic surface-enhanced Raman spectroscopic (M-SERS) dots for the identification of bronchioalveolar stem cells in normal and lung cancer mice.
Bronchioalveolar stem cells (BASCs) play an important role in the development of cancer. To study the characterization of BASCs, their isolation and purification are important. However, the cells are very rare in tissues and the available methods of isolating them are limited. The current study was performed to isolate BASCs in the murine lung using magnetic nanoparticle-based surface-enhanced Raman spectroscopic dots (M-SERS Dots). We used K-ras(LA1) mice, a laboratory animal model of non-small cell lung cancer of human, and C57BL/6 mice having the same age as a control. We compared the BASCs between 2 species by FACS analysis with 4 markers of BASCs, CCSP, SP-C, CD34, and Sca-1. We found that BASCs were more abundant in the K-ras(LA1) mice than in the C57BL/6 mice. Also, the M-SERS Dot-mediated positive selection of the CD34(pos) cells enabled the BASCs to be enriched to an approximately 4- to 5-fold higher level than that in the case without pre-separation. In summary, our study demonstrates the potential of using M-SERS Dots as a sorting system with very effective isolation of BASCs and multiplex targeting probe, showing that they may play an effective role in the study of BASCs in the future.
Noh
Mi Suk
MS
Laboratory of Toxicology, College of Veterinary Medicine, Seoul National University, Seoul 151-742, Republic of Korea.
Jun
Bong-Hyun
BH
Kim
Seongyong
S
Kang
Homan
H
Woo
Min-Ah
MA
Minai-Tehrani
Arash
A
Kim
Ji-Eun
JE
Kim
Jaeyun
J
Park
Jooyoung
J
Lim
Hwang-Tae
HT
Park
Se-Chang
SC
Hyeon
Taeghwan
T
Kim
Yong-Kweon
YK
Jeong
Dae Hong
DH
Lee
Yoon-Sik
YS
Cho
Myung-Haing
MH
D000818:Animals; D001980:Bronchi / Q000166:cytology*; D045744:Cell Line, Tumor; D002478:Cells, Cultured; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D006801:Humans; D008175:Lung Neoplasms / Q000473:pathology*; D008280:Magnetics; D051379:Mice; D008810:Mice, Inbred C57BL; D053758:Nanoparticles / Q000737:chemistry*; D011650:Pulmonary Alveoli / Q000166:cytology*; D013059:Spectrum Analysis, Raman / Q000379:methods*; D013234:Stem Cells / Q000166:cytology*