Phylogenetic classification of heterotrophic bacteria associated with filamentous marine cyanobacteria in culture.
Fifty-one heterotrophic bacterial strains were isolated from the marine cyanobacterial cultures of heterocystous Nodularia harveyana strain Bo53 and non-heterocystous Oscillatoria brevis strain Bo10. Fluorescence in situ hybridisation and fingerprinting methods were used for a preliminary taxonomical classification of 44 of the 51 isolates. The strains obtained from Bo53 were mostly Alphaproteobacteria (10/24), followed by Bacteroidetes (7/24), and Gammaproteobacteria (3/24). The affiliation of the isolates originating from Bo10 was dominated by Alphaproteobacteria (8/20) and Bacteroidetes (7/20), followed by Gammaproteobacteria (3/20). The 16S rRNA genes of four selected isolates were sequenced. A red-coloured bacterium from Bo53 grouped with the alphaproteobacterial genus Porphyrobacter, while the other three strains, obtained from Bo10, belonged to the alphaproteobacterial genera Roseobacter (pink) and Rhodobacter (colourless), and to the genus Muricauda (yellow) of Bacteroidetes. The findings indicated that the aerobic anoxygenic phototroph Porphyrobacter and its relatives only occurred in Bo10 culture, whereas members of the Roseobacter clade and the Bacteroidetes bacterium Muricauda sp. seemed to be more ubiquitous.
Universität Bremen, Zentrum für Umweltforschung und nachhaltige Technologien (UFT) and Fachbereich Biologie/Chemie, Abteilung Marine Mikrobiologie, Leobener Str., D-28359 Bremen, Germany. ahube@marmic.mpg.de
Annina Elisabeth
AE
Hube
Birgit
B
Heyduck-Söller
Ulrich
U
Fischer
D020561:Alphaproteobacteria / Q000145:classification / Q000166:cytology / Q000235:genetics / Q000302:isolation & purification; D001419:Bacteria / Q000145:classification* / Q000166:cytology / Q000235:genetics / Q000302:isolation & purification; D041963:Bacteroidetes / Q000145:classification / Q000166:cytology / Q000235:genetics / Q000302:isolation & purification; D001483:Base Sequence; D002478:Cells, Cultured; D000458:Cyanobacteria / Q000166:cytology / Q000254:growth & development*; D017753:Ecosystem; D052836:Heterotrophic Processes; D008969:Molecular Sequence Data; D046932:Nodularia / Q000166:cytology / Q000254:growth & development; D054300:Oscillatoria / Q000166:cytology / Q000254:growth & development; D010802:Phylogeny; D041902:Rhodobacteraceae / Q000145:classification / Q000166:cytology / Q000235:genetics / Q000302:isolation & purification; D042301:Sphingomonadaceae / Q000145:classification / Q000166:cytology / Q000235:genetics / Q000302:isolation & purification