iTRAQ quantitative analysis of Francisella tularensis ssp. holarctica live vaccine strain and Francisella tularensis ssp. tularensis SCHU S4 response to different temperatures and stationary phases of growth.
Proteomics has been shown to significantly contribute to the investigation of the pathogenicity of the extremely infectious bacteria Francisella tularensis. In this study, the authors employed iTRAQ quantitative proteomic analysis in order to monitor alterations in proteomes of F. tularensis ssp. holarctica live vaccine strain and F. tularensis ssp. tularensis SCHU S4 associated with the cultivation at different temperatures or in the stationary phase. Correlated production of the identified proteins studied by the exploratory statistical analysis revealed novel candidates for virulence factors that were regulated in a similar manner to the genes encoded in the Francisella Pathogenicity Island. Moreover, the assessment of the adaptation of live vaccine strain and SCHU S4 strain to the examined stimuli uncovered differences in their physiological responses to the stationary phase of growth.
Institute of Molecular Pathology, Faculty of Military Health Sciences, University of Defence, Trebesska 1575, Hradec Kralove, Czech Republic. lenco@pmfhk.cz
Juraj
J
Lenco
Marek
M
Link
Vojtech
V
Tambor
Jitka
J
Zaková
Lukas
L
Cerveny
And Jiri
AJ
Stulik
D001426:Bacterial Proteins / Q000378:metabolism*; D002853:Chromatography, Liquid; D005604:Francisella tularensis / Q000145:classification / Q000254:growth & development* / Q000378:metabolism*; D025341:Principal Component Analysis; D040901:Proteomics / Q000379:methods*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013696:Temperature; D037521:Virulence Factors / Q000378:metabolism*
D001426:Bacterial Proteins; D037521:Virulence Factors