Visualization of aberrant perinuclear microtubule aster organization by microtubule-destabilizing agents.
To analyze aberrant spindle formation by microtubule-targeting drugs, live cell imaging was performed using multi-fluorescent human MDA-MB-435 cells in which several spindle components were visualized. Time-lapse images revealed that nocodazole and vinblastine induced additional perinuclear asters at the onset of mitosis. These results imply that these drugs stimulate the microtubule-organizing activity, despite their microtubule-destabilizing properties.
Laboratory of Molecular Biology and Cell Informatics, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Sakai, Osaka, Japan. ssaka@biochem.osakafu-u.ac.jp
Shinji
S
Sakaushi
Kaori
K
Senda-Murata
Shigenori
S
Oka
Kenji
K
Sugimoto
D045744:Cell Line, Tumor; D002467:Cell Nucleus / Q000187:drug effects* / Q000378:metabolism; D048750:Cell Nucleus Division / Q000187:drug effects; D002470:Cell Survival; D004305:Dose-Response Relationship, Drug; D006801:Humans; D008870:Microtubules / Q000187:drug effects* / Q000378:metabolism*; D015739:Nocodazole / Q000494:pharmacology; D017239:Paclitaxel / Q000494:pharmacology; D050257:Tubulin Modulators / Q000494:pharmacology*; D014747:Vinblastine / Q000494:pharmacology
D050257:Tubulin Modulators; D014747:Vinblastine; D017239:Paclitaxel; D015739:Nocodazole