Protection from Staphylococcus aureus mastitis associated with poly-N-acetyl beta-1,6 glucosamine specific antibody production using biofilm-embedded bacteria.
Staphylococcus aureus vaccines based on bacterins surrounded by slime, surface polysaccharides coupled to protein carriers and polysaccharides embedded in liposomes administered together with non-biofilm bacterins confer protection against mastitis. However, it remains unknown whether protective antibodies are directed to slime-associated known exopolysaccharides and could be produced in the absence of bacterin immunizations. Here, a sheep mastitis vaccination study was carried out using bacterins, crude bacterial extracts or a purified exopolysaccharide from biofilm bacteria delivered in different vehicles. This polysaccharide reacted specifically with antibodies to poly-N-acetyl-beta-1,6-glucosamine (PNAG) and not with antibodies to other capsular antigens or bacterial components. Following intra-mammary challenge with biofilm-producing bacteria, antibody production against the polysaccharide, milk bacterial counts and mastitis lesions were determined. Bacterins from strong biofilm-producing bacteria triggered the highest production of antibodies to PNAG and conferred the highest protection against infection and mastitis, compared with weak biofilm-producing bacteria and non-cellular inocula. Thus, bacterins from strong biofilm bacteria, rather than purified polysaccharide, are proposed as a cost-efficient vaccination against S. aureus ruminant mastitis.
Departamento de Sanidad Animal, SIA-CITA (DGA) Ctra. de Montañana, Zaragoza, Spain.
M M
MM
Pérez
A
A
Prenafeta
J
J
Valle
J
J
Penadés
C
C
Rota
C
C
Solano
J
J
Marco
M J
MJ
Grilló
I
I
Lasa
J M
JM
Irache
T
T
Maira-Litran
J
J
Jiménez-Barbero
L
L
Costa
G B
GB
Pier
D
D
de Andrés
B
B
Amorena
D000818:Animals; D000917:Antibody Formation; D018441:Biofilms; D005260:Female; D008321:Mammary Glands, Animal / Q000473:pathology; D008413:Mastitis / Q000209:etiology / Q000473:pathology / Q000517:prevention & control*; D008892:Milk / Q000382:microbiology; D011247:Pregnancy; D012756:Sheep; D013203:Staphylococcal Infections / Q000150:complications / Q000276:immunology / Q000473:pathology / Q000517:prevention & control; D013209:Staphylococcal Vaccines / Q000627:therapeutic use*; D013211:Staphylococcus aureus / Q000502:physiology*; D016896:Treatment Outcome; D047071:beta-Glucans / Q000276:immunology*
D013209:Staphylococcal Vaccines; D047071:beta-Glucans; C505465:poly-N-acetyl-1-6-glucosamine