Protective effect of green tea extract on gentamicin-induced nephrotoxicity and oxidative damage in rat kidney.
Gentamicin (GM) is an effective aminoglycoside antibiotic against severe infections but nephrotoxicity and oxidative damage limits its long term clinical use. Various strategies were attempted to ameliorate GM nephropathy but were not found suitable for clinical practice. Green tea (GT) polyphenols have shown strong chemopreventive and chemotherapeutic effects against various pathologies. We hypothesized that GT prevents GM nephrotoxicity by virtue of its antioxidative properties. A nephrotoxic dose of GM was co-administered to control and GT-fed male Wistar rats. Serum parameters and enzymes of oxidative stress, brush border membrane (BBM), and carbohydrate metabolism were analyzed. GM increased serum creatinine, cholesterol, blood urea nitrogen (BUN), lipid peroxidation (LPO) and suppressed superoxide dismutase (SOD) and catalase activities in renal tissues. Activity of hexokinase, lactate dehydrogenase increased whereas malate dehydrogenase decreased. Gluconeogenic enzymes and glucose-6-phosphate dehydrogenase were differentially altered in the cortex and medulla. However, GT given to GM rats reduced nephrotoxicity parameters, enhanced antioxidant defense and energy metabolism. The activity of BBM enzymes and transport of Pi declined by GM whereas GT enhanced BBM enzymes and Pi transport. In conclusion, green tea ameliorates GM elicited nephrotoxicity and oxidative damage by improving antioxidant defense, tissue integrity and energy metabolism.
Department of Biochemistry, Faculty of Life Sciences, Aligarh Muslim University, Aligarh 202002, U.P., India.
Sara A
SA
Khan
Shubha
S
Priyamvada
Neelam
N
Farooq
Sheeba
S
Khan
Md Wasim
MW
Khan
Ahad N K
AN
Yusufi
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D050260:Carbohydrate Metabolism / Q000187:drug effects; D016166:Free Radical Scavengers / Q000378:metabolism; D005839:Gentamicins / Q000009:adverse effects*; D007668:Kidney / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D007673:Kidney Cortex Necrosis / Q000139:chemically induced* / Q000201:enzymology / Q000517:prevention & control*; D015227:Lipid Peroxidation / Q000187:drug effects; D008247:Lysosomes / Q000378:metabolism; D008297:Male; D008871:Microvilli / Q000201:enzymology / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects*; D010710:Phosphates / Q000378:metabolism; D010936:Plant Extracts / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D013438:Sulfhydryl Compounds / Q000378:metabolism; D013662:Tea
D000975:Antioxidants; D016166:Free Radical Scavengers; D005839:Gentamicins; D010710:Phosphates; D010936:Plant Extracts; D013438:Sulfhydryl Compounds; D013662:Tea