An analytical method for the determination of perfluorinated compounds in whole blood using acetonitrile and solid phase extraction methods.
A method for the analysis of perfluorinated compounds (perfluoroalkyl sulfonates: C4, C6, C8, C10; perfluoroalkyl sulfinates: C6, C8, C10; perfluorooctanesulfonamide, N-ethyl perfluorooctanesulfonamide, N-ethyl perfluorooctanesulfonamidoacetate, perfluorocarboxylates: C4-C14; fluorotelomer carboxylate (7:3, 8:2) in whole blood using acetonitrile and OASIS WAX solid phase extraction (SPE) cartridge was developed. Separation of target compounds in two HPLC columns (ion exchange JJ50-2D and C18 Betasil columns) was examined. Matrix recoveries of the developed methods ranged from 70% to 120%. Separation of possible inferences such as taurodeoxycholic acid (TDC) was accomplished using an ion exchange JJ50-2D column, and this separation was validated using whole blood of different animals.
National Institute of Advanced Industrial Science and Technology, Tsukuba, Ibaraki, Japan.
Leo W Y
LW
Yeung
Sachi
S
Taniyasu
Kurunthachalam
K
Kannan
Della Z Y
DZ
Xu
Keerthi S
KS
Guruge
Paul K S
PK
Lam
Nobuyoshi
N
Yamashita
D000097:Acetonitriles / Q000737:chemistry*; D017738:Alkanesulfonic Acids / Q000097:blood*; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D005466:Fluorocarbons / Q000097:blood*; D005561:Formates / Q000737:chemistry; D006801:Humans; D015203:Reproducibility of Results; D052616:Solid Phase Extraction / Q000379:methods*; D013449:Sulfonamides / Q000097:blood*; D013657:Taurodeoxycholic Acid / Q000097:blood
D000097:Acetonitriles; D017738:Alkanesulfonic Acids; D005466:Fluorocarbons; D005561:Formates; D013449:Sulfonamides; C030544:formic acid; D013657:Taurodeoxycholic Acid; C032159:acetonitrile