Cytoprotective constituents of the heartwood of Caesalpinia sappan on glutamate-induced oxidative damage in HT22 cells.
The bioassay-guided fractionation of a MeOH extract of the heartwood of Caesalpinia sappan L. provided two neuroprotective compounds, sappanchalcone (2) and 4-O-methylepisappanol (3), together with a methoxychalcone, isoliquiritigenin 2'-methyl ether (1), and three aromatic compounds, 4-O-methylsappanol (4), caesalpine J (5), pluchoic acid (6). At concentrations of 20-40 microM, compound 2 showed significant cytoprotective effects against glutamate-induced oxidative stress through the induction of heme oxygenase (HO)-1 in HT22-immortalized hippocampal cells. Compound 3 also showed moderate neuroprotective effect at 40 microM, but compounds 1, 4-6 did not show any protective effects against glutamate-induced cytotoxicity in HT22 cells.
Institute of Radiological Imaging Science, Wonkwang University, Iksan, Korea.
Gil-Saeng
GS
Jeong
Dong-Sung
DS
Lee
Tae-Oh
TO
Kwon
Hye-Suk
HS
Lee
Ren-Bo
RB
An
Youn-Chul
YC
Kim
D000818:Animals; D015153:Blotting, Western; D029911:Caesalpinia / Q000737:chemistry*; D002460:Cell Line; D002467:Cell Nucleus / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism; D002470:Cell Survival / Q000187:drug effects; D002599:Chalcone / Q000031:analogs & derivatives* / Q000302:isolation & purification / Q000494:pharmacology; D003600:Cytosol / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D018698:Glutamic Acid / Q000009:adverse effects*; D051547:Heme Oxygenase-1 / Q000096:biosynthesis; D006624:Hippocampus / Q000166:cytology; D051379:Mice; D015394:Molecular Structure; D018696:Neuroprotective Agents / Q000302:isolation & purification / Q000494:pharmacology*; D018384:Oxidative Stress / Q000187:drug effects*; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000378:metabolism; D013329:Structure-Activity Relationship; D014934:Wood
D018696:Neuroprotective Agents; D010936:Plant Extracts; D017382:Reactive Oxygen Species; C550177:isoliquiritigenin 2'-methyl ether; D018698:Glutamic Acid; D002599:Chalcone; D051547:Heme Oxygenase-1