Digital in-line holography with femtosecond VUV radiation provided by the free-electron laser FLASH.
Femtosecond vacuum ultraviolet (VUV) radiation provided by the free-electron laser FLASH was used for digital in-line holographic microscopy and applied to image particles, diatoms and critical point dried fibroblast cells. To realize the classical in-line Gabor geometry, a 1 microm pinhole was used as spatial filter to generate a divergent light cone with excellent pointing stability. At a fundamental wavelength of 8 nm test objects such as particles and diatoms were imaged at a spatial resolution of 620 nm. In order to demonstrate the applicability to biologically relevant systems, critical point dried rat embryonic fibroblast cells were for the first time imaged with free-electron laser radiation.
Angewandte Physikalische Chemie, Universität Heidelberg, Heidelberg, Germany. rosenhahn@uni-heidelberg.de
Axel
A
Rosenhahn
Florian
F
Staier
Thomas
T
Nisius
David
D
Schäfer
Ruth
R
Barth
Christof
C
Christophis
Lorenz-M
LM
Stadler
Simone
S
Streit-Nierobisch
Christian
C
Gutt
Adrian
A
Mancuso
Andreas
A
Schropp
Johannes
J
Gulden
Bernd
B
Reime
Josef
J
Feldhaus
Edgar
E
Weckert
Bastian
B
Pfau
Christian M
CM
Günther
René
R
Könnecke
Stefan
S
Eisebitt
Michael
M
Martins
Bart
B
Faatz
Natalia
N
Guerassimova
Katja
K
Honkavaara
Rolf
R
Treusch
Evgueni
E
Saldin
Siegfried
S
Schreiber
Evgeny A
EA
Schneidmiller
Mikhail V
MV
Yurkov
Ivan
I
Vartanyants
Gerhard
G
Grübel
Michael
M
Grunze
Thomas
T
Wilhein
D000818:Animals; D017377:Diatoms / Q000166:cytology; D004583:Electrons; D006696:Holography / Q000379:methods*; D007834:Lasers; D051381:Rats; D012822:Silicon Dioxide; D013997:Time Factors; D014466:Ultraviolet Rays; D014618:Vacuum
D012822:Silicon Dioxide