Effects of resveratrol on H(2)O(2)-induced apoptosis and expression of SIRTs in H9c2 cells.
Resveratrol, a polyphenol found in fruits, has been demonstrated to activate Sir2. Though many studies have demonstrated that resveratrol can activate SIRT1, whether it has effect on other sirtuins (SIRT2-7) are unknown. The present study shows that exposure of H9c2 cells to 50 microM H(2)O(2) for 6 h caused a significant increase in apoptosis, as evaluated by TUNEL and flow cytometry (FCM), but pretreatment of resveratrol (20 microM) eliminated H(2)O(2)-induced apoptosis. Resveratrol also prevented H(2)O(2)-induced caspase-3 activation. Exposure of cells to resveratrol caused rapid activation of SIRT1,3,4,7. Sirtuin inhibitor, nicotinamide (20 mM) attenuated resveratrol's inhibitory effect on cell apoptosis and caspase-3 activity. These results suggest that resveratrol protects cardiomyocytes from H(2)O(2)-induced apoptosis by activating SIRT1,3,4,7.
Department of Cardiology, First Affiliated Hospital of Shantou University Medical College, China.
Wei
W
Yu
Yu-Cai
YC
Fu
Xiao-Hui
XH
Zhou
Chun-Juan
CJ
Chen
Xin
X
Wang
Rui-Bo
RB
Lin
Wei
W
Wang
D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D053148:Caspase 3 / Q000378:metabolism; D002460:Cell Line; D005786:Gene Expression Regulation / Q000187:drug effects*; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D032383:Myocytes, Cardiac / Q000166:cytology / Q000187:drug effects*; D051381:Rats; D000077185:Resveratrol; D056564:Sirtuin 1; D037761:Sirtuins / Q000235:genetics*; D013267:Stilbenes / Q000494:pharmacology*
D013267:Stilbenes; D006861:Hydrogen Peroxide; D053148:Caspase 3; C488368:Sirt1 protein, rat; D056564:Sirtuin 1; D037761:Sirtuins; D000077185:Resveratrol