Estrogen receptor alpha interacts with 17beta-hydroxysteroid dehydrogenase type 10 in mitochondria.
Estrogen receptor alpha (ERalpha) is present in the nucleus, the cytosol and in mitochondria. The rat ERalpha ligand binding domain was employed as bait in a bacterial two-hybrid screening of a human heart cDNA library to detect novel protein-protein interaction partners of ERalpha in the heart. 17beta-Hydroxysteroid dehydrogenase type 10 (17beta-HSD10), which converts potent (17beta-estradiol) to less potent estrogens (estrone), co-localized with 17beta-HSD10 in the mitochondria of rat cardiac myocytes. GST pull-down experiments confirmed the interaction of ERalpha and 17beta-HSD10. These findings suggest that the ERalpha estrogen receptor might be involved in regulating intracellular estrogen levels by modulating 17beta-HSD10 activity.
Laboratory of Molecular Cardiology, Department of Medicine, University Clinics Wuerzburg, Josef-Schneider-Strasse 2, D20, 97080 Wuerzburg, Germany. Jazbutyte_V@klinik.uni-wuerzburg.de
Virginija
V
Jazbutyte
Franz
F
Kehl
Ludwig
L
Neyses
Theo
T
Pelzer
D015094:3-Hydroxyacyl CoA Dehydrogenases / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D002467:Cell Nucleus / Q000201:enzymology; D047628:Estrogen Receptor alpha / Q000235:genetics / Q000378:metabolism*; D015723:Gene Library; D006801:Humans; D047468:Immunoprecipitation; D008929:Mitochondria, Heart / Q000201:enzymology*; D032383:Myocytes, Cardiac / Q000201:enzymology* / Q000648:ultrastructure; D051381:Rats; D020798:Two-Hybrid System Techniques
D047628:Estrogen Receptor alpha; D015094:3-Hydroxyacyl CoA Dehydrogenases; C108634:HSD17B10 protein, human