[Synergistic effect of feruloyl esterase and cellulase in hydrolyzation of steam-exploded rice straw].
Ferulic acid esterase (FAE) was used to hydrolyze feruloyl ester linkages between hemicellulose and lignin in natural lignocellulose, and the possibility of FAE accelerating cellulase to hydrolyze steam-exploded rice straw by breaking covalent linkages was studied. When the dosage of FAE was 240 mu/g substrate, the cellulose conversion rate and the weight-loss rate of insoluble substrate at 72 h were respectively 32.00% and 32.77%, more than without using FAE; Cellulose conversion rate and the weight-loss rate of insoluble substrate were respectively 29.85% and 32.48% with FAE (300 mu/g substrate) processing time of 120 min. By comparison of the accessibility and FT-IR spectra of lignocellulosic material treated by different enzyme methods, it indicated that FAE hydrolyzed some ester bonds within it, and improved the accessibility by over 50%. It is concluded that FAE and cellulase have great synergistic effect, and FAE can help cellulase hydrolyze natural lignocellulose and enhance hydrolytic efficiency.
National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China.
Wei
W
Zeng
Hongzhang
H
Chen
D002265:Carboxylic Ester Hydrolases / Q000378:metabolism*; D002480:Cellulase / Q000378:metabolism*; D002482:Cellulose / Q000378:metabolism*; D012275:Oryza / Q000201:enzymology*; D018547:Plant Stems / Q000201:enzymology; D013227:Steam
D013227:Steam; D002482:Cellulose; D002265:Carboxylic Ester Hydrolases; C070196:feruloyl esterase; D002480:Cellulase