Characterization of one typical 2-Cys peroxiredoxin gene of Taenia solium and Taenia crassiceps.
The Taenia genus is capable of living for long periods within its hosts. Reports have shown that this successful establishment is related to its efficient defense mechanisms against host immune response and its high tolerance to oxidative stress. In this work, we describe the genomic sequences of one Taenia solium and Taenia crassiceps typical 2-Cys peroxiredoxins (Ts2-CysPrx, Tc2-CysPrx) genes, which are 94% identical in primary sequence with the typical 2-Cys Prxs catalytic motifs. Both genes have the same genomic architecture, showing a TATA box and Initiator (Inr) sequence in their proximal promoter, two exons split by a 67-bp type III intron and one unique transcription start site located inside the Inr. We show that T. crassiceps cysticerci are highly tolerant to H(2)O(2) presenting a lethal concentration 50 of 3.0 mM and demonstrate that the typical Tc2-CysPrx gene is not induced by H(2)O(2), showing a behavior of an antioxidant housekeeping gene. This study describes for first time the gene structure of a typical 2-Cys Prx in the Taenia genus.
Departamento de Microbiología y Parasitología, Facultad de Medicina, Universidad Nacional Autónoma de México, Edificio A 2do piso, Ciudad Universitaria, Mexico City, 04510, Mexico.
Felipe
F
Vaca-Paniagua
Ricardo
R
Parra-Unda
Abraham
A
Landa
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D018509:DNA, Helminth / Q000737:chemistry / Q000235:genetics; D005091:Exons; D020869:Gene Expression Profiling; D015801:Helminth Proteins / Q000235:genetics*; D006861:Hydrogen Peroxide / Q000633:toxicity; D007438:Introns; D007928:Lethal Dose 50; D008969:Molecular Sequence Data; D054464:Peroxiredoxins / Q000096:biosynthesis / Q000235:genetics*; D011401:Promoter Regions, Genetic; D012045:Regulatory Sequences, Nucleic Acid; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D016019:Survival Analysis; D013621:Taenia / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics; D024363:Transcription Initiation Site
D018509:DNA, Helminth; D015801:Helminth Proteins; D006861:Hydrogen Peroxide; D054464:Peroxiredoxins