Choline up-regulates BDNF and down-regulates TrkB neurotrophin receptor in rat cortical cell culture.
In this study, possible involvements of choline and nicotinic acetylcholine receptors (nAChRs) in neurotrophic-related neuronal plasticity were investigated. Primary cell cultures from rat cerebral cortex were exposed for 72 h to the alpha7 nAChR selective agonist choline and protein expression levels of the neurotrophin receptors p75, TrkA, TrkB and TrkC were examined. The results revealed a choline-induced attenuation of the TrkB expression, whereas the other neurotrophin receptors were not affected. Further analysis of choline-exposed cell cultures showed an increased protein level of the TrkB ligand brain-derived neurotrophic factor (BDNF). This increase was obtained in cell cultures where the alpha7 nAChR subunit was detected, but not in younger cell cultures where this subunit could not be detected. It is speculated that a choline-induced change of alpha7 nAChRs activity may have resulted in the observed increase of BDNF level and down-regulation of the TrkB receptor.
Johansson
Jan
J
Department of Medicine and Public Health, Section of Pharmacology, University of Verona, Piazzale L. Scuro, Verona, Italy.
Formaggio
Elena
E
Fumagalli
Guido
G
Chiamulera
Christian
C
D000818:Animals; D019208:Brain-Derived Neurotrophic Factor / Q000378:metabolism*; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000166:cytology / Q000378:metabolism*; D002794:Choline / Q000378:metabolism* / Q000494:pharmacology; D015536:Down-Regulation / Q000502:physiology; D009419:Nerve Tissue Proteins; D009473:Neuronal Plasticity / Q000187:drug effects / Q000502:physiology*; D018722:Nicotinic Agonists / Q000378:metabolism / Q000494:pharmacology; D051381:Rats; D020917:Receptor, trkA / Q000187:drug effects / Q000378:metabolism; D020813:Receptor, trkB / Q000378:metabolism*; D017978:Receptors, Growth Factor; D017475:Receptors, Nerve Growth Factor / Q000187:drug effects / Q000378:metabolism; D011978:Receptors, Nicotinic / Q000187:drug effects / Q000378:metabolism*; D015398:Signal Transduction / Q000187:drug effects / Q000502:physiology; D015854:Up-Regulation / Q000502:physiology; D064569:alpha7 Nicotinic Acetylcholine Receptor
D019208:Brain-Derived Neurotrophic Factor; C579165:Chrna7 protein, rat; D009419:Nerve Tissue Proteins; D018722:Nicotinic Agonists; D017978:Receptors, Growth Factor; D017475:Receptors, Nerve Growth Factor; D011978:Receptors, Nicotinic; D064569:alpha7 Nicotinic Acetylcholine Receptor; C068313:Ngfr protein, rat; D020917:Receptor, trkA; D020813:Receptor, trkB; D002794:Choline