NMR assignment of polymerase beta labeled with 2H, 13C, and 15N in complex with substrate DNA.
DNA polymerase beta is a multifunctional enzyme involved in base excision repair of nuclear DNA invertebrate cells. We present here the first assignments of the full-length protein (335 residues, 39 kDa) in the presence of a double gap--double hairpin DNA (22 nucleotides, 7 kDa).
National Institute of Environmental Health Sciences, Research Triangle Park, NC 23455, USA.
Geoffrey A
GA
Mueller
Eugene F
EF
DeRose
Thomas W
TW
Kirby
Robert E
RE
London
D000818:Animals; D001483:Base Sequence; D004247:DNA / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D019951:DNA Polymerase beta / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D066298:In Vitro Techniques; D019906:Nuclear Magnetic Resonance, Biomolecular; D009690:Nucleic Acid Conformation; D051381:Rats; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D013379:Substrate Specificity
D011994:Recombinant Proteins; D004247:DNA; D019951:DNA Polymerase beta