Safety and healing efficacy of Sea buckthorn (Hippophae rhamnoides L.) seed oil on burn wounds in rats.
The present investigation was undertaken to determine the safety and efficacy of supercritical CO2-extracted Hippophae rhamnoides L. (Sea buckthorn) (SBT) seed oil on burn wound model. SBT seed oil was co-administered by two routes at a dose of 2.5 ml/kg body weight (p.o.) and 200 microl (topical) for 7 days on experimental burn wounds in rats. The SBT seed oil augmented the wound healing process as indicated by significant increase in wound contraction, hydroxyproline, hexosamine, DNA and total protein contents in comparison to control and reference control treated with silver sulfadiazine (SS) ointment. Histopathological findings further confirmed the healing potential of SBT seed oil. SBT seed oil treatment up-regulated the expression of matrix metalloproteinases (MMP-2 and 9), collagen type-III and VEGF in granulation tissue. It was observed that SBT seed oil also possesses antioxidant properties as evidenced by significant increase in reduced glutathione (GSH) level and reduced production of reactive oxygen species (ROS) in wound granulation tissue. In acute and sub-acute oral toxicity studies, no adverse effects were observed in any of the groups administered with SBT seed oil. These results suggest that the supercritical CO2-extracted Sea buckthorn seed oil possesses significant wound healing activity and have no associated toxicity or side effects.
Department of Biochemical Pharmacology, Defence Institute of Physiology and Allied Sciences, Timarpur, Delhi 110054, India.
N K
NK
Upadhyay
R
R
Kumar
S K
SK
Mandotra
R N
RN
Meena
M S
MS
Siddiqui
R C
RC
Sawhney
A
A
Gupta
D000818:Animals; D000891:Anti-Infective Agents, Local / Q000627:therapeutic use; D001772:Blood Cell Count; D001774:Blood Chemical Analysis; D015153:Blotting, Western; D001835:Body Weight / Q000187:drug effects; D002056:Burns / Q000188:drug therapy* / Q000473:pathology; D002245:Carbon Dioxide; D025924:Chromatography, Supercritical Fluid; D004591:Electrophoresis, Polyacrylamide Gel; D005978:Glutathione / Q000378:metabolism; D031281:Hippophae / Q000737:chemistry*; D007509:Irritants / Q000633:toxicity; D008297:Male; D020782:Matrix Metalloproteinases / Q000378:metabolism; D009929:Organ Size / Q000187:drug effects; D010938:Plant Oils / Q000627:therapeutic use* / Q000633:toxicity*; D051381:Rats; D017207:Rats, Sprague-Dawley; D017382:Reactive Oxygen Species / Q000032:analysis; D012639:Seeds / Q000737:chemistry; D012837:Silver Sulfadiazine / Q000627:therapeutic use
D000891:Anti-Infective Agents, Local; D007509:Irritants; D010938:Plant Oils; D017382:Reactive Oxygen Species; D002245:Carbon Dioxide; D020782:Matrix Metalloproteinases; D005978:Glutathione; D012837:Silver Sulfadiazine