A novel pseudovirus containing lewis X oligosaccharides with pD-L1 targeting to DCs induces T cells tolerance.
The purpose of this study was to assess the potential of a novel pseudovirus targeting to dendritic cells (DCs) inducing T cells tolerance. To prove it, allogeneic DCs transfected of the novel pseudovirus containing Lewis X oligosaccharides with programmed death-ligand 1 were co-cultured with T cells. After mixed leukocyte reactions were performed, the proliferation, cytokines secretion, and activation marker expression of T cells were analyzed. The results demonstrated that the proliferation, cytokines secretion, and activation marker expression of T cells were suppressed after co-cultured with the pseudovirus transfected DCs. These findings suggested that DCs transfected with pseudovirus could induce T-cells tolerance, which provided a promising therapeutic means to negatively manipulate immune response in organ transplantation.
Respiratory Department of Daping Hospital, Third Military Medical University, Chongqing, People's Republic of China.
Xingsheng
X
Wang
Xuemei
X
Yang
Shehuai
S
Cui
Yiqun
Y
Yan
Zhao
Z
Yang
D018920:Coculture Techniques; D003713:Dendritic Cells / Q000166:cytology / Q000276:immunology*; D006801:Humans; D007108:Immune Tolerance; D007983:Lewis Blood Group Antigens; D008213:Lymphocyte Activation; D009844:Oligosaccharides / Q000276:immunology; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology*; D014162:Transfection; D014780:Viruses / Q000235:genetics
D007983:Lewis Blood Group Antigens; C494219:Lewis a oligosaccharide; D009844:Oligosaccharides