Contributions of apoplasmic cadmium accumulation, antioxidative enzymes and induction of phytochelatins in cadmium tolerance of the cadmium-accumulating cultivar of black oat (Avena strigosa Schreb.).
The contributions of cadmium (Cd) accumulation in cell walls, antioxidative enzymes and induction of phytochelatins (PCs) to Cd tolerance were investigated in two distinctive genotypes of black oat (Avena strigosa Schreb.). One cultivar of black oat 'New oat' accumulated Cd in the leaves at the highest concentration compared to another black oat cultivar 'Soil saver' and other major graminaceous crops. The shoot:root Cd ratio also demonstrated that 'New oat' was the high Cd-accumulating cultivar, whereas 'Soil saver' was the low Cd-accumulating cultivar. Varied levels of Cd exposure demonstrated the strong Cd tolerance of 'New oat'. By contrast, low Cd-accumulating cultivar 'Soil saver' suffered Cd toxicity such as growth defects and increased lipid peroxidation, even though it accumulated less Cd in shoots than 'New oat'. Higher activities of ascorbate peroxidase (EC 1.11.1.11) and superoxide dismutase (EC 1. 15. 1. 1) were observed in the leaves of 'New oat' than in 'Soil saver'. No advantage of 'New oat' in PCs induction was observed in comparison to Cd-sensitive cultivar 'Soil saver', although Cd exposure increased the concentration of total PCs in both cultivars. Higher and increased Cd accumulation in cell wall fraction was observed in shoots of 'New oat'. On the other hand, in 'Soil saver', apoplasmic Cd accumulation showed saturation under higher Cd exposure. Overall, the present results suggest that cell wall Cd accumulation and antioxidative activities function in the tolerance against Cd stress possibly in combination with vacuolar Cd compartmentation.
Laboratory of Environmental Toxicology, Institute of Symbiotic Science and Technology, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai-cho, Fuchu, Tokyo, 183-8509, Japan. shim@bu.iij4u.or.jp
Shimpei
S
Uraguchi
Masako
M
Kiyono
Takuya
T
Sakamoto
Izumi
I
Watanabe
Katsuji
K
Kuno
D060387:Ascorbate Peroxidases; D002104:Cadmium / Q000378:metabolism* / Q000633:toxicity*; D002473:Cell Wall / Q000187:drug effects / Q000378:metabolism; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D015227:Lipid Peroxidation / Q000187:drug effects; D010544:Peroxidases / Q000378:metabolism; D054811:Phytochelatins / Q000378:metabolism*; D018515:Plant Leaves / Q000187:drug effects / Q000378:metabolism; D018520:Plant Shoots / Q000187:drug effects / Q000378:metabolism; D006109:Poaceae / Q000187:drug effects* / Q000378:metabolism*; D012989:Soil Pollutants / Q000633:toxicity*; D013482:Superoxide Dismutase / Q000378:metabolism
D012989:Soil Pollutants; D002104:Cadmium; D054811:Phytochelatins; D010544:Peroxidases; D060387:Ascorbate Peroxidases; D013482:Superoxide Dismutase