Detection of Toxoplasma gondii oocysts in water: proposition of a strategy and evaluation in Champagne-Ardenne Region, France.
Water is a vehicle for disseminating human and veterinary toxoplasmosis due to oocyst contamination. Several outbreaks of toxoplasmosis throughout the world have been related to contaminated drinking water. We have developed a method for the detection of Toxoplasma gondii oocysts in water and we propose a strategy for the detection of multiple waterborne parasites, including Cryptosporidium spp. and Giardia. Water samples were filtered to recover Toxoplasma oocysts and, after the detection of Cryptosporidium oocysts and Giardia cysts by immunofluorescence, as recommended by French norm procedure NF T 90-455, the samples were purified on a sucrose density gradient. Detection of Toxoplasma was based on PCR amplification and mouse inoculation to determine the presence and infectivity of recovered oocysts. After experimental seeding assays, we determined that the PCR assay was more sensitive than the bioassay. This strategy was then applied to 482 environmental water samples collected since 2001. We detected Toxoplasma DNA in 37 environmental samples (7.7%), including public drinking water; however, none of them were positive by bioassay. This strategy efficiently detects Toxoplasma oocysts in water and may be suitable as a public health sentinel method. Alternative methods can be used in conjunction with this one to determine the infectivity of parasites that were detected by molecular methods.
Laboratoire de Parasitologie-Mycologie, Centre National de Référence de Toxoplasmose, CHU Maison Blanche, UFR Médecine, Reims Cédex, France.
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Aubert
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Villena
D000818:Animals; D016054:DNA, Protozoan / Q000032:analysis*; D005374:Filtration / Q000379:methods; D005602:France; D006801:Humans; D051379:Mice; D033661:Oocysts; D016133:Polymerase Chain Reaction; D014122:Toxoplasma / Q000302:isolation & purification*; D014867:Water / Q000469:parasitology*; D014881:Water Supply
D016054:DNA, Protozoan; D014867:Water