Magnetic surface-enhanced Raman spectroscopic (M-SERS) dots for the identification of bronchioalveolar stem cells in normal and lung cancer mice.
Bronchioalveolar stem cells (BASCs) play an important role in the development of cancer. To study the characterization of BASCs, their isolation and purification are important. However, the cells are very rare in tissues and the available methods of isolating them are limited. The current study was performed to isolate BASCs in the murine lung using magnetic nanoparticle-based surface-enhanced Raman spectroscopic dots (M-SERS Dots). We used K-ras(LA1) mice, a laboratory animal model of non-small cell lung cancer of human, and C57BL/6 mice having the same age as a control. We compared the BASCs between 2 species by FACS analysis with 4 markers of BASCs, CCSP, SP-C, CD34, and Sca-1. We found that BASCs were more abundant in the K-ras(LA1) mice than in the C57BL/6 mice. Also, the M-SERS Dot-mediated positive selection of the CD34(pos) cells enabled the BASCs to be enriched to an approximately 4- to 5-fold higher level than that in the case without pre-separation. In summary, our study demonstrates the potential of using M-SERS Dots as a sorting system with very effective isolation of BASCs and multiplex targeting probe, showing that they may play an effective role in the study of BASCs in the future.
Laboratory of Toxicology, College of Veterinary Medicine, Seoul National University, Seoul 151-742, Republic of Korea.
Mi Suk
MS
Noh
Bong-Hyun
BH
Jun
Seongyong
S
Kim
Homan
H
Kang
Min-Ah
MA
Woo
Arash
A
Minai-Tehrani
Ji-Eun
JE
Kim
Jaeyun
J
Kim
Jooyoung
J
Park
Hwang-Tae
HT
Lim
Se-Chang
SC
Park
Taeghwan
T
Hyeon
Yong-Kweon
YK
Kim
Dae Hong
DH
Jeong
Yoon-Sik
YS
Lee
Myung-Haing
MH
Cho
D000818:Animals; D001980:Bronchi / Q000166:cytology*; D045744:Cell Line, Tumor; D002478:Cells, Cultured; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D006801:Humans; D008175:Lung Neoplasms / Q000473:pathology*; D008280:Magnetics; D051379:Mice; D008810:Mice, Inbred C57BL; D053758:Nanoparticles / Q000737:chemistry*; D011650:Pulmonary Alveoli / Q000166:cytology*; D013059:Spectrum Analysis, Raman / Q000379:methods*; D013234:Stem Cells / Q000166:cytology*