Neural induction in mouse embryonic stem cells by co-culturing with chicken somites.
The role of paraxial mesoderm or the somites in decision of ectoderm to acquire the neuroepithelial fate and its subsequent diversification to functional neural subtypes especially in mammalians is obscure. Here we report, for the first time, the influence of the co-culture of alginate bead-encapsulated somites isolated from chick embryos on neural differentiation in mouse embryonic stem cells-derived embryoid bodies (EBs). Using a combination of morphology, immunofluorescence, flow cytometry, semiquantitative, and conventional RT-PCR techniques, we show that the somites induce rosette structures and weakly enhance neural differentiation and neural markers in a dose-dependent manner in comparison to the control group, but its effect is lower than retinoic acid treatment of EBs. By liquid chromatography-mass spectrometry, it was revealed that somitic cells synthesized and released retinoids into the medium. However, no effective influence of somitic co-culture on rostrocaudal or dorsoventral patterning is seen. Therefore, like amphibians, somites have a modest neural-inducing activity in amniotes.
Department of Stem Cells, Cell Science Research Center, Royan Institute, Esfahan Campus, Iranian Academic Center for Education, Culture, and Research (ACECR), Esfahan, Iran.
Mohsen
M
Sagha
Khadijeh
K
Karbalaie
Somaieh
S
Tanhaee
Ebrahim
E
Esfandiari
Hossein
H
Salehi
Hojjat
H
Sadeghi-Aliabadi
Shahnaz
S
Razavi
Mohammad Hossein
MH
Nasr-Esfahani
Hossein
H
Baharvand
D000818:Animals; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D002478:Cells, Cultured; D002642:Chick Embryo; D002645:Chickens; D002853:Chromatography, Liquid; D018920:Coculture Techniques; D053595:Embryonic Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D020869:Gene Expression Profiling; D013058:Mass Spectrometry; D051379:Mice; D009474:Neurons / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D012176:Retinoids / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D019170:Somites / Q000166:cytology*; D014212:Tretinoin / Q000494:pharmacology
D012176:Retinoids; D014212:Tretinoin