Magnetic chitosan nanoparticles as a drug delivery system for targeting photodynamic therapy.
Photodynamic therapy (PDT) has become an increasingly recognized alternative to cancer treatment in clinic. However, PDT therapy agents, namely photosensitizer (PS), are limited in application as a result of prolonged cutaneous photosensitivity, poor water solubility and inadequate selectivity, which are encountered by numerous chemical therapies. Magnetic chitosan nanoparticles provide excellent biocompatibility, biodegradability, non-toxicity and water solubility without compromising their magnetic targeting. Nevertheless, no previous attempt has been reported to develop an in vivo magnetic drug delivery system with chitosan nanoparticles for magnetic resonance imaging (MRI) monitored targeting photodynamic therapy. In this study, magnetic targeting chitosan nanoparticles (MTCNPs) were prepared and tailored as a drug delivery system and imaging agents for PS, designated as PHPP. Results showed that PHPP-MTCNPs could be used in MRI monitored targeting PDT with excellent targeting and imaging ability. Non-toxicity and high photodynamic efficacy on SW480 carcinoma cells both in vitro and in vivo were achieved with this method at the level of 0-100 microM. Notably, localization of nanoparticles in skin and hepatic tissue was significantly less than in tumor tissue, therefore photosensitivity and hepatotoxicity can be attenuated.
Sun
Yun
Y
Department of Pharmaceutical Science and Technology, College of Chemistry and Biology, Donghua University, Shanghai 201620, People's Republic of China.
Chen
Zhi-Long
ZL
Yang
Xiao-Xia
XX
Huang
Peng
P
Zhou
Xin-Ping
XP
Du
Xiao-Xia
XX
D000818:Animals; D045744:Cell Line, Tumor; D048271:Chitosan; D016503:Drug Delivery Systems / Q000379:methods*; D004574:Electromagnetic Fields; D006801:Humans; D008279:Magnetic Resonance Imaging / Q000379:methods*; D051379:Mice; D008819:Mice, Nude; D046529:Microscopy, Electron, Transmission; D053758:Nanoparticles / Q000627:therapeutic use* / Q000648:ultrastructure; D010778:Photochemotherapy / Q000379:methods*; D011166:Porphyrins; D023041:Xenograft Model Antitumor Assays
D011166:Porphyrins; D048271:Chitosan