Phosphorylation dependence of hsp27 multimeric size and molecular chaperone function.
The molecular chaperone Hsp27 exists as a distribution of large oligomers that are disassembled by phosphorylation at Ser-15, -78, and -82. It is controversial whether the unphosphorylated Hsp27 or the widely used triple Ser-to-Asp phospho-mimic mutant is the more active molecular chaperone in vitro. This question was investigated here by correlating chaperone activity, as measured by the aggregation of reduced insulin or alpha-lactalbumin, with Hsp27 self-association as monitored by analytical ultracentrifugation. Furthermore, because the phospho-mimic is generally assumed to reproduce the phosphorylated molecule, the size and chaperone activity of phosphorylated Hsp27 were compared with that of the phospho-mimic. Hsp27 was triply phosphorylated by MAPKAP-2 kinase, and phosphorylation was tracked by urea-PAGE. An increasing degree of suppression of insulin or alpha-lactalbumin aggregation correlated with a decreasing Hsp27 self-association, which was the least for phosphorylated Hsp27 followed by the mimic followed by the unphosphorylated protein. It was also found that Hsp27 added to pre-aggregated insulin did not reverse aggregation but did inhibit these aggregates from assembling into even larger aggregates. This chaperone activity appears to be independent of Hsp27 phosphorylation. In conclusion, the most active chaperone of insulin and alpha-lactalbumin was the Hsp27 (elongated) dimer, the smallest Hsp27 subunit observed under physiological conditions. Next, the Hsp27 phospho-mimic is only a partial mimic of phosphorylated Hsp27, both in self-association and in chaperone function. Finally, the efficient inhibition of insulin aggregation by Hsp27 dimer led to the proposal of two models for this chaperone activity.
Boston Biomedical Research Institute, Watertown, Massachusetts 02472, USA.
David
D
Hayes
Vanessa
V
Napoli
Andrew
A
Mazurkie
Walter F
WF
Stafford
Philip
P
Graceffa
D003432:Cross-Linking Reagents / Q000737:chemistry; D004220:Disulfides / Q000737:chemistry; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000378:metabolism; D055551:HSP27 Heat-Shock Proteins / Q000737:chemistry*; D006360:Heat-Shock Proteins / Q000737:chemistry; D007328:Insulin / Q000378:metabolism; D007768:Lactalbumin / Q000737:chemistry; D020935:MAP Kinase Signaling System; D018832:Molecular Chaperones / Q000737:chemistry*; D010766:Phosphorylation; D017510:Protein Folding; D012441:Saccharomyces cerevisiae / Q000378:metabolism; D013997:Time Factors; D014461:Ultracentrifugation
D003432:Cross-Linking Reagents; D004220:Disulfides; D055551:HSP27 Heat-Shock Proteins; D006360:Heat-Shock Proteins; D007328:Insulin; D018832:Molecular Chaperones; D007768:Lactalbumin