Detection of circulating antigen in serum of mice infected with Schistosoma japonicum by immunomagnetic bead ELISA based on IgY.
We developed a novel immunomagnetic bead ELISA based on IgY (egg yolk immunoglobulin) for detection of circulating antigen (CA) in sera of mice infected with Schistosoma japonicum. The assay involved the use of chicken polyclonal antibodies IgY against soluble egg antigens (SEA) of S. japonicum as a capture antibody and anti-SEA mouse monoclonal antibody NP28-5B labeled horseradish peroxidase (HRP) as a detecting antibody. Two groups of BALB/c mice infected with S. japonicum cercariae were used: lightly infected mice (infected with 10 S. japonicum cercariae) and heavily infected mice (infected with 30 S. japonicum cercariae). The CA was detectable as early as 4 and 5 weeks after infection in the sera of heavily and lightly infected mice, respectively. The CA levels rose rapidly and reached a peak in 8 weeks after infection and then remained a plateau for at least another 6 weeks in both groups. Moreover, the effect of praziquantel on the CA levels was also investigated. The heavily infected mice were treated with praziquantel and the CA levels in sera increased dramatically in the first week post-treatment and then decreased to the control level by 6 weeks after treatment. The novel assay appears to be sensitive for detection of schistosomal antigenemia and valuable to judge the efficacy of chemotherapy in murine schistosomiasis.
Lei
Jia-hui
JH
Department of Parasitology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, PR China.
Liu
Wen-qi
WQ
Sun
Cheng-song
CS
Tang
Chun-lian
CL
Li
Man-jun
MJ
Chen
Yu-li
YL
Li
Yong-long
YL
D000818:Animals; D000871:Anthelmintics / Q000627:therapeutic use; D000909:Antibodies, Helminth; D000911:Antibodies, Monoclonal; D000947:Antigens, Helminth / Q000097:blood*; D002645:Chickens; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005260:Female; D006735:Horseradish Peroxidase / Q000378:metabolism; D007136:Immunoglobulins; D051379:Mice; D008807:Mice, Inbred BALB C; D011223:Praziquantel / Q000627:therapeutic use; D012549:Schistosoma japonicum / Q000276:immunology / Q000302:isolation & purification*; D012552:Schistosomiasis / Q000175:diagnosis* / Q000188:drug therapy / Q000469:parasitology; D013194:Staining and Labeling
D000871:Anthelmintics; D000909:Antibodies, Helminth; D000911:Antibodies, Monoclonal; D000947:Antigens, Helminth; C016512:IgY; D007136:Immunoglobulins; D011223:Praziquantel; D006735:Horseradish Peroxidase