Suppression of allergic diarrhea in murine ovalbumin-induced allergic diarrhea model by PG102, a water-soluble extract prepared from Actinidia arguta.
BACKGROUND
Allergic reactions to food can involve diarrhea, vomiting, nausea and abnormal pain. PG102 has previously been shown to control various factors involved in allergy pathogenesis, including IgE and various Th1 and Th2 cytokines, in vivo as well as in vitro [Park EJ, et al.: J Allergy Clin Immunol 2005;116:1151-1157; Park EJ, et al.: J Invest Dermatol 2007;127:1154-1160]. These data indicate that PG102 might have antiallergic effects on allergic diarrhea. Here, we investigated whether PG102 could prevent allergic diarrhea in the murine ovalbumin (OVA)-induced allergic diarrhea model.


METHODS
BALB/c mice were orally treated with PG102, dexamethasone or water for 9 days on a daily basis, followed by subcutaneous injection with OVA on day 0. Animals were orally administrated with OVA from day 7, 3 times a week, over a period of approximately 20 days. Incidence of diarrhea, serum, OVA-restimulated splenocytes and lamina propria lymphocytes were analyzed.


RESULTS
Oral administration of PG102 could suppress the incidence of diarrhea in a murine allergic diarrhea model. The amelioration of allergic diarrhea by PG102 was accompanied with the inhibition of mast cell infiltration into the large intestine. The serum level of IgE, IL-6 and MCP-1 was decreased in PG102-treated mice. When splenocytes were isolated from respective groups and cultured in the presence of OVA, cells from PG102-administrated animals produced lesser amounts of IL-6 and MCP-1.


CONCLUSIONS
PG102 has the potential to be used as a preventive for food allergic diseases.
Kim
Donghyun
D
School of Biological Sciences, Seoul National University, Seoul, Korea.
Kim
Seon Hee
SH
Park
Eun-Jin
EJ
Kim
Jiyoung
J
Cho
Sang-Heon
SH
Kagawa
Junko
J
Arai
Naoko
N
Jun
Kunisawa
K
Kiyono
Hiroshi
H
Kim
Sunyoung
S
D029042:Actinidia / Q000737:chemistry*; D000818:Animals; D000001:Calcimycin / Q000494:pharmacology; D002465:Cell Movement / Q000187:drug effects / Q000276:immunology; D018932:Chemokine CCL2 / Q000097:blood / Q000378:metabolism; D003907:Dexamethasone / Q000627:therapeutic use; D003967:Diarrhea / Q000188:drug therapy* / Q000276:immunology* / Q000473:pathology; D004195:Disease Models, Animal; D005260:Female; D005512:Food Hypersensitivity / Q000188:drug therapy* / Q000276:immunology / Q000473:pathology; D007073:Immunoglobulin E / Q000097:blood / Q000276:immunology; D016753:Interleukin-10 / Q000378:metabolism; D015847:Interleukin-4 / Q000378:metabolism; D015850:Interleukin-6 / Q000097:blood / Q000378:metabolism; D007420:Intestine, Large / Q000276:immunology / Q000473:pathology; D008214:Lymphocytes / Q000187:drug effects / Q000276:immunology / Q000378:metabolism; D008407:Mast Cells / Q000187:drug effects / Q000276:immunology / Q000473:pathology; D051379:Mice; D008807:Mice, Inbred BALB C; D009092:Mucous Membrane / Q000473:pathology; D010047:Ovalbumin / Q000008:administration & dosage / Q000276:immunology*; D010936:Plant Extracts / Q000494:pharmacology / Q000627:therapeutic use*; D051381:Rats; D013154:Spleen / Q000166:cytology
C491768:Ccl2 protein, mouse; D018932:Chemokine CCL2; D015850:Interleukin-6; C519391:PG102T extract; D010936:Plant Extracts; D016753:Interleukin-10; D015847:Interleukin-4; D007073:Immunoglobulin E; D000001:Calcimycin; D003907:Dexamethasone; D010047:Ovalbumin