[Effects of excess pyridoxine-HCl on growth and urinary excretion of water-soluble vitamins in weaning rats].
To determine the tolerable upper intake level of pyridoxine-HCl in humans, we investigated the effects of excess pyridoxine-HCl administration on body weight gain, food intake, tissue weight, and urinary excretion of water-soluble vitamins in weaning rats. The weaning rats were freely fed ordinary diet containing 0.0007% pyridoxine-HCl (control diet) or the same diet with 0.1%, 0.5%, 0.8% or 1.0% pyridoxine-HCl for 30 days. The body weight gain in the 0.8% and 1.0% groups, and the total food intake in the 1.0% group were significantly lower than those in the control group. The urinary excretion of pantothenic acid in the pyridoxine-HCl added groups were higher than that in the control group, while excessive pyridoxine-HCl intake did not affect the urinary excretion of other water-soluble vitamins. These results showed that the no-observed-adverse-effect-level (NOAEL) for pyridoxine-HCl was 0.1% in diet, corresponding to 90 mg/kg body weight/day, and lowest-observed-adverse-effect-level (LOAEL) was 0.5% in diet, corresponding to 450 mg/kg body weight/day.
Department of Food Science and Nutrition, School of Human Cultures, The University of Shiga Prefecture, Hikone, Shiga 5228533, Japan.
Tsutomu
T
Fukuwatari
Keiko
K
Itoh
Katsumi
K
Shibata
D000818:Animals; D004435:Eating / Q000187:drug effects; D006128:Growth / Q000187:drug effects*; D008297:Male; D009929:Organ Size / Q000187:drug effects; D010205:Pantothenic Acid / Q000652:urine; D011736:Pyridoxine / Q000633:toxicity*; D051381:Rats; D017208:Rats, Wistar; D012016:Reference Values; D014815:Vitamins / Q000652:urine*; D015430:Weight Gain / Q000187:drug effects
D014815:Vitamins; D010205:Pantothenic Acid; D011736:Pyridoxine