Bioelectrochemical sensing based on single stranded deoxyribonucleic acid-carbon nanotubes covalently attached on gold electrodes.
A biosensor based on single-stranded deoxyribonucleic acid-functionalized carbon nanotubes covalently attached to a self-assembled monolayer of 11-amino-1-undecanethiol on gold has been prepared. The preparation of the deoxyribonucleic acid sensor was followed using cyclic voltammetry. Single-walled carbon nanotubes, covalently attached to the gold surface present a nanoelectrode array behavior. To confirm the hybridization step between the probe and the target deoxyribonucleic acid, we used methylene blue, which is an indicator capable of distinguishing between single-stranded deoxyribonucleic acid and double-stranded deoxyribonucleic acid. Our results demonstrate that the modification of gold with single-walled carbon nanotubes and single-stranded deoxyribonucleic acid can be used for deoxyribonucleic acid hybridization detection.
Department of Chemistry and Center for Nanoscale Materials, University of Puerto Rico, Rio Piedras Campus, P.O. Box 23346, San Juan, Puerto Rico 00931-3346.
Lenibel
L
Santiago-Rodríguez
Nella M
NM
Vargas-Barbosa
Germarie
G
Sánchez-Pomales
Carlos R
CR
Cabrera
D000473:Alkanes / Q000138:chemical synthesis / Q000737:chemistry; D015374:Biosensing Techniques / Q000379:methods*; D004247:DNA / Q000378:metabolism*; D004277:DNA, Single-Stranded / Q000138:chemical synthesis / Q000737:chemistry / Q000378:metabolism*; D004563:Electrochemistry / Q000379:methods; D004566:Electrodes; D006046:Gold / Q000737:chemistry*; D008751:Methylene Blue / Q000378:metabolism; D037742:Nanotubes, Carbon / Q000737:chemistry*; D009693:Nucleic Acid Hybridization; D013438:Sulfhydryl Compounds / Q000138:chemical synthesis / Q000737:chemistry
C510729:11-amino-1-undecanethiol; D000473:Alkanes; D004277:DNA, Single-Stranded; D037742:Nanotubes, Carbon; D013438:Sulfhydryl Compounds; D006046:Gold; D004247:DNA; D008751:Methylene Blue