Characterization of the 5' regulatory region of the human Glycine N-methyltransferase gene.
Glycine N-methyltransferase (GNMT) is a tumor susceptibility gene for both hepatocellular carcinoma and prostate cancer. We have previously characterized GNMT genomic structure and mapped its chromosomal localization to 6p12. For this study we identified a GNMT transcriptional start site at the 14th position upstream of the ATG codon. Electrophoretic mobility shift assay results indicate binding of the nuclear factor-Y (NF-Y) transcription factor to the CCAAT box (-71/-67) of the GNMT gene. Mutation assay results suggest that the nucleotide sequence in the -56/-47 region is a binding site for a putative transcriptional factor. The TATA-less core promoter (-133/+14) contains three major elements: an Sp1 site, CCAAT box, and a novel box within the CTGTCGGCTG sequence. One functional xenobiotic response element (XRE) located at the -104/-82 region is inducable by benzo[a]pyrene treatment. We believe our results have value for the study of GNMT transcriptional regulation.
Lee
Cheng-Ming
CM
AIDS Prevention and Research Center, National Yang-Ming University, Taipei 112, Taiwan.
Shih
Yi-Ping
YP
Wu
Cho-Han
CH
Chen
Yi-Ming Arthur
YM
D001483:Base Sequence; D023081:CCAAT-Binding Factor / Q000378:metabolism; D024202:Electrophoretic Mobility Shift Assay; D050938:Glycine N-Methyltransferase / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D012045:Regulatory Sequences, Nucleic Acid; D024363:Transcription Initiation Site
D023081:CCAAT-Binding Factor; C494510:GNMT protein, human; D050938:Glycine N-Methyltransferase