[Protecting effect of mesenteric lymph duct ligation on vital organs of rats with hemorrhagic shock].
OBJECTIVE
To investigate the protecting effect of mesenteric lymph duct ligation on cytokine expression and pathological changes in intestine, liver and lung in rats with hemorrhagic shock.


METHODS
Twenty-four Sprague-Dawley (SD) rats were randomly divided into three groups: control, hemorrhagic shock and hemorrhagic shock plus mesenteric lymph duct ligation, with 8 rats in each group. Reverse transcription-polymerase chain reaction (RT-PCR) was used to detect the expressions of tumor necrosis factor-alpha (TNF-alpha) mRNA and interleukin-6 (IL-6) mRNA in intestine, liver and lung tissue in three groups. Hematoxylin-eosin (HE) staining was used for the observation of pathological changes in intestine, liver, lung in all groups.


RESULTS
The TNF-alpha mRNA and IL-6 mRNA in intestinal, liver, and lung tissues of rats in the hemorrhagic shock group were markedly higher than those in control group (TNF-alpha mRNA: intestine 0.54+/-0.07 vs. 0.37+/-0.05, liver 1.01+/-0.06 vs. 0.56+/-0.07, lung 0.94+/-0.07 vs. 0.62+/-0.06; IL-6 mRNA: intestine 0.89+/-0.12 vs. 0.50+/-0.09, liver 1.07+/-0.10 vs. 0.57+/-0.12, lung 1.09+/-0.09 vs. 0.67+/-0.06, all P<0.01). Mesenteric lymph duct ligation could obviously reduce the expressions of TNF-alpha mRNA and IL-6 mRNA (TNF-alpha mRNA: intestine 0.47+/-0.05 vs. 0.54+/-0.07, liver 0.81+/-0.07 vs. 1.01+/-0.06, lung 0.80+/-0.05 vs. 0.94+/-0.07; IL-6 mRNA: intestine 0.66+/-0.07 vs. 0.89+/-0.12, liver 0.83+/-0.13 vs. 1.07+/-0.10, lung 0.73+/-0.11 vs. 1.09+/-0.09, P<0.05 or P<0.01). Mesenteric lymph duct ligation could markedly attenuate necrosis and exfoliation of the intestinal villi, and it also ameliorated degeneration of liver cells, lung edema and infiltration of inflammatory cells.


CONCLUSIONS
Mesenteric lymph duct ligation can decrease the expressions of TNF-alpha mRNA and IL-6 mRNA in intestine, liver and lung tissue and attenuate the damage to these organs in rats with hemorrhagic shock. It plays a vital role in protecting the organ functions.
Surgical Intensive Care Unit, First Affiliated Hospital of SUN Yat-Sen University, Guangzhou 510080, Guangdong, China.
Yong-jun
YJ
Liu
En-qiang
EQ
Mao
Bin
B
Ouyang
Juan
J
Chen
Yao-qing
YQ
Tang
Xiang-dong
XD
Guan
D000818:Animals; D004195:Disease Models, Animal; D015850:Interleukin-6 / Q000378:metabolism; D007413:Intestinal Mucosa / Q000378:metabolism; D007422:Intestines / Q000473:pathology; D008026:Ligation; D008099:Liver / Q000378:metabolism / Q000473:pathology; D008168:Lung / Q000378:metabolism / Q000473:pathology; D042601:Lymphatic Vessels / Q000601:surgery*; D008297:Male; D008643:Mesentery / Q000601:surgery; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D012771:Shock, Hemorrhagic / Q000378:metabolism / Q000473:pathology / Q000601:surgery*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
D015850:Interleukin-6; D014409:Tumor Necrosis Factor-alpha