Relaxin signaling in uterine fibroids.
Uterine fibroid is the most common tumor of female reproductive organs. The role of relaxin signaling in leiomyoma development was analyzed. We used 23 matched pairs of leiomyoma and normal myometrium samples to compare the expression of relaxin family peptide receptors RXFP1 and RXFP2, caveolin 1, desmin, and steroid receptors and their cofactors, NCOR1 and NCOR2. The expression of RXFP1, evaluated by quantitative reverse transcription-PCR, was downregulated in fibroid tissues. Relaxin or insulin-like peptide 3 treatment suppressed transforming growth factor beta-induced phosphorylation of SMAD2 in rat leiomyoma ELT-3 cells in vitro, suggesting a possible involvement of relaxins in the etiology of leiomyoma.
Department of Obstetrics and Gynecology, Baylor College of Medicine, Houston, Texas 77025, USA.
Zhen
Z
Li
Jennifer K
JK
Burzawa
Anne
A
Troung
Shu
S
Feng
Irina U
IU
Agoulnik
Xiaowen
X
Tong
Matthew L
ML
Anderson
Ertug
E
Kovanci
Aleksandar
A
Rajkovic
Alexander I
AI
Agoulnik
D000328:Adult; D000818:Animals; D015153:Blotting, Western; D051242:Caveolin 1 / Q000235:genetics / Q000378:metabolism; D045744:Cell Line, Tumor; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D003893:Desmin / Q000235:genetics / Q000378:metabolism; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D007889:Leiomyoma / Q000378:metabolism*; D008875:Middle Aged; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism; D056971:Nuclear Receptor Co-Repressor 1; D056985:Nuclear Receptor Co-Repressor 2; D010766:Phosphorylation / Q000187:drug effects; D051381:Rats; D043562:Receptors, G-Protein-Coupled / Q000235:genetics / Q000378:metabolism; D018000:Receptors, Peptide / Q000235:genetics / Q000378:metabolism; D012065:Relaxin / Q000378:metabolism / Q000494:pharmacology / Q000502:physiology*; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction; D051899:Smad2 Protein / Q000378:metabolism; D016212:Transforming Growth Factor beta / Q000494:pharmacology
D051242:Caveolin 1; D004268:DNA-Binding Proteins; D003893:Desmin; C539127:NCOR1 protein, human; C539130:NCOR2 protein, human; C539129:Ncor1 protein, rat; C539132:Ncor2 protein, rat; D009687:Nuclear Proteins; D056971:Nuclear Receptor Co-Repressor 1; D056985:Nuclear Receptor Co-Repressor 2; C417543:RXFP1 protein, human; C472867:RXFP2 protein, human; D043562:Receptors, G-Protein-Coupled; D018000:Receptors, Peptide; D012097:Repressor Proteins; D051899:Smad2 Protein; C497784:Smad2 protein, rat; D016212:Transforming Growth Factor beta; D012065:Relaxin