Assessment of process variables on 2-ethylhexyl palmitate production using Novozym 435 as catalyst in a solvent-free system.
This work reports the optimization of 2-ethylhexyl palmitate production by esterification reaction in a solvent-free system using a commercial lipase as catalyst. For this, a sequential strategy was performed applying three experimental designs. An empirical model was built so as to assess the effects of process variables on the reaction conversion. Afterward, the operating conditions that optimized 2-ethylhexyl palmitate production were determined to be acid to alcohol molar ratio of 1:5.5, 70 degrees C, 150 rpm and 10.5 wt% of enzyme, leading to a reaction conversion as high as 93%. From this point, a kinetic study was carried out evaluating the influence of acid to alcohol molar ratio, enzyme concentration and temperature on product yield. Results obtained in this step allow to conclude that an excess of alcohol (acid to alcohol molar ratio of 1:6), relatively low enzyme concentration (10 wt%) and temperature of 70 degrees C led to nearly complete reaction conversion.
Richetti
Aline
A
Escola de Química, EQ/UFRJ, CT, Bloco E, Rio de Janeiro, RJ 21945-900, Brazil.
Leite
Selma G F
SG
Antunes
Octávio A C
OA
Lerin
Lindomar A
LA
Dallago
Rogério M
RM
Emmerich
Daniel
D
Di Luccio
Marco
M
Vladimir Oliveira
J
J
Treichel
Helen
H
de Oliveira
Débora
D
D000438:Alcohols / Q000737:chemistry; D001709:Biotechnology / Q000379:methods*; D002175:Candida / Q000201:enzymology; D002384:Catalysis; D004800:Enzymes, Immobilized / Q000737:chemistry; D004952:Esters / Q000737:chemistry; D005656:Fungal Proteins; D007700:Kinetics; D008049:Lipase / Q000737:chemistry*; D009930:Organic Chemicals / Q000737:chemistry; D010168:Palmitates / Q000737:chemistry*; D012997:Solvents / Q000737:chemistry*; D013696:Temperature; D013997:Time Factors
D000438:Alcohols; D004800:Enzymes, Immobilized; D004952:Esters; D005656:Fungal Proteins; D009930:Organic Chemicals; D010168:Palmitates; D012997:Solvents; C032207:ethylhexyl palmitate; C093667:Novozyme 435; D008049:Lipase