Characterization of a new myeloid leukemia cell line with normal cytogenetics (CG-SH).
A new myeloid leukemia cell line (CG-SH) with normal cytogenetics was established from a patient with acute myelogenous leukemia (AML) following myelodysplastic syndrome (MDS). The cells of CG-SH are immature blasts and have an immature myeloid phenotype (positive for myeloperoxidase, CD7, CD34, CD38, CD117, HLA-DR, negative for CD10, CD19, CD20, CD41, CD42). A partial expression of CD13, CD15, CD65 and a weak expression of CD33 and CD133 was noted. The cells are negative for EBER. By molecular analysis, a mutation of NRAS and heterozygous mutations of RUNX1 were detected. No mutations were detected in FLT3-ITD, MLL-PTD or NPM1. By real-time PCR, a series of 19 microRNAs was identified which are strongly expressed in CG-SH. In conclusion, a new cell line was established which will be useful for the study of AML with normal cytogenetics and mutations in NRAS and/or RUNX1.
Department of Medicine, the Louisiana State University Health Sciences Center, Shreveport, LA 71130, USA. rmunker@lsuhsc.edu
Reinhold
R
Munker
Mary Lowery
ML
Nordberg
Diana
D
Veillon
B Jill
BJ
Williams
Anthony
A
Roggero
Wolfgang
W
Kern
Frank
F
Dicker
Torsten
T
Haferlach
D015703:Antigens, CD / Q000235:genetics; D045744:Cell Line, Tumor; D002869:Chromosome Aberrations; D050676:Core Binding Factor Alpha 2 Subunit / Q000235:genetics; D020732:Cytogenetic Analysis / Q000379:methods*; D016207:Cytokines / Q000235:genetics; D011905:Genes, ras; D006680:HLA Antigens / Q000235:genetics; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007951:Leukemia, Myeloid / Q000235:genetics*; D015470:Leukemia, Myeloid, Acute / Q000235:genetics*; D035683:MicroRNAs / Q000235:genetics; D009154:Mutation; D000090243:Nucleophosmin; D010641:Phenotype; D012334:RNA, Neoplasm / Q000235:genetics; D012016:Reference Values
D015703:Antigens, CD; D050676:Core Binding Factor Alpha 2 Subunit; D016207:Cytokines; D006680:HLA Antigens; D035683:MicroRNAs; C000716967:NPM1 protein, human; D012334:RNA, Neoplasm; C493728:RUNX1 protein, human; D000090243:Nucleophosmin