Bioassay-guided isolation and identification of desacetylmicrocolin B from Lyngbya cf. polychroa.
Bioassay-guided fractionation of a non-polar extract of Lyngbya cf. polychroa resulted in the isolation of the cytotoxic desacetylmicrocolin B (1) as well as the known compounds microcolins A (2) and B (3). Compound 1 was found to inhibit the growth of HT-29 colorectal adenocarcinoma and IMR-32 neuroblastoma cells with half maximal inhibitory concentration (IC(50)) values of 14 nM for both cancer cell types. Microcolins A and B were found to have little activity against two strains of the marine fungus Dendryphiella salina with LD(50) values above 200 microg/mL. Compounds 1, 2, and 3 were obtained by reverse-phase chromatography and their structures were determined by NMR and MS. In this paper we report the isolation, identification, and biological activity of 1.
Smithsonian Marine Station at Fort Pierce, Fort Pierce, Florida 34949, USA.
Theresa
T
Meickle
Susan
S
Matthew
Cliff
C
Ross
Hendrik
H
Luesch
Valerie
V
Paul
D000230:Adenocarcinoma / Q000188:drug therapy; D000972:Antineoplastic Agents, Phytogenic / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use*; D001681:Biological Assay; D045744:Cell Line, Tumor; D003110:Colonic Neoplasms / Q000188:drug therapy; D000458:Cyanobacteria / Q000737:chemistry*; D005658:Fungi / Q000187:drug effects; D006801:Humans; D020128:Inhibitory Concentration 50; D008235:Lyngbya Toxins / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use*; D015394:Molecular Structure; D009369:Neoplasms / Q000188:drug therapy*; D009447:Neuroblastoma / Q000188:drug therapy; D009842:Oligopeptides / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use*; D011759:Pyrrolidines / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use*; D012004:Rectal Neoplasms / Q000188:drug therapy
D000972:Antineoplastic Agents, Phytogenic; D008235:Lyngbya Toxins; D009842:Oligopeptides; D011759:Pyrrolidines; C547859:desacetylmicrocolin B