Metabolic phenotyping of genetically modified mice: An NMR metabonomic approach.
Monocyte chemoattractant protein-1 (MCP-1) plays a relevant role in macrophage migration but recent findings suggest an additional role in lipid and glucose metabolism. We report the use of (1)H NMR spectroscopy as a useful complementary method to assess the metabolic function of this gene in a comparative strategy. This metabonomic analysis was rapid, simple, quantitative and reproducible, and revealed a suggestive relationship between the expression of the MCP-1 gene and hepatic glucose and taurine concentrations. This approach should be considered in genetically modified mice when a metabolic alteration is suspected, or in routine assessment of metabolic phenotype.
Rull
Anna
A
Centre de Recerca Biomèdica, Hospital Universitari Sant Joan de Reus, IISPV, Universitat Rovira i Virgili, Spain.
Vinaixa
Maria
M
Angel Rodríguez
Miguel
M
Beltrán
Raúl
R
Brezmes
Jesús
J
Cañellas
Nicolau
N
Correig
Xavier
X
Joven
Jorge
J
D000818:Animals; D008099:Liver / Q000737:chemistry / Q000166:cytology / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D055432:Metabolomics / Q000379:methods*; D051379:Mice / Q000235:genetics* / Q000378:metabolism*; D008822:Mice, Transgenic; D008970:Molecular Weight; D010641:Phenotype; D014867:Water / Q000737:chemistry
D014867:Water