A mutation in the Proteosomal Regulatory Particle AAA-ATPase-3 in Arabidopsis impairs the light-specific hypocotyl elongation response elicited by a glutamate receptor agonist, BMAA.
BMAA is a cycad-derived glutamate receptor agonist that causes a two- to three-fold increase in hypocotyl elongation on Arabidopsis seedlings grown in the light. To probe the role of plant glutamate receptors and their downstream mediators, we utilized a previously described genetic screen to identify a novel, BMAA insensitive morphology (bim) mutant, bim409. The normal BMAA-induced hypocotyl elongation response observed on wild-type seedlings grown in the light is impaired in the bim409 mutant. This BMAA-induced phenotype is light-specific, as the bim409 mutant exhibits normal hypocotyl elongation in etiolated (dark grown) plants (+ or - BMAA). The mutation in bim409 was identified to be in a gene encoding the Proteosomal Regulatory Particle AAA-ATPase-3 (RPT3). Possible roles of the proteosome in Glu-mediated signaling in plants is discussed.
Brenner
Eric D
ED
The International Plant Science Center, The New York Botanical Garden, 200th and Kazimiroff Blvd., Bronx, NY 10458, USA. ebrenner@nybg.org
Feinberg
Philip
P
Runko
Suzan
S
Coruzzi
Gloria M
GM
D000251:Adenosine Triphosphatases / Q000737:chemistry / Q000235:genetics*; D000595:Amino Acid Sequence; D000599:Amino Acids, Diamino / Q000494:pharmacology*; D017360:Arabidopsis / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development; D029681:Arabidopsis Proteins / Q000737:chemistry / Q000235:genetics; D001483:Base Sequence; D001665:Binding Sites / Q000235:genetics; D000087522:Cyanobacteria Toxins; D004305:Dose-Response Relationship, Drug; D018690:Excitatory Amino Acid Agonists / Q000494:pharmacology; D005808:Genes, Recessive; D018546:Hypocotyl / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009154:Mutation; D010641:Phenotype; D046988:Proteasome Endopeptidase Complex / Q000737:chemistry / Q000235:genetics; D017434:Protein Structure, Tertiary; D017386:Sequence Homology, Amino Acid
D000599:Amino Acids, Diamino; D029681:Arabidopsis Proteins; D000087522:Cyanobacteria Toxins; D018690:Excitatory Amino Acid Agonists; C001824:beta-N-methylamino-L-alanine; D046988:Proteasome Endopeptidase Complex; D000251:Adenosine Triphosphatases