Intracellular localization of CD14 protein in intestinal macrophages.
BACKGROUND
Our research is focused on intestinal macrophages, which are believed to contribute to the maintenance of intestinal homeostasis. In addition, intestinal macrophages are unique in that there is an impairment of expression of tumor necrosis factor (TNF) from lipopolysaccharide (LPS). This characteristic can be attributed to the lack or poor level of expression of toll-like receptor 4 (TLR4) or CD14 on the membrane of intestinal macrophages. We therefore decided to identify where CD14 was localized in intestinal macrophages.


METHODS
The endoplasmic reticulum and Golgi apparatus were double stained and the intracellular localization in the intestinal macrophages was observed using a confocal laser microscope.


RESULTS
CD14 of peritoneal macrophages was expressed both in the endoplasmic reticulum and Golgi apparatus. By contrast, intestinal macrophages expressed very little CD14 on the cellular membrane. CD14 was present in the endoplasmic reticulum of intestinal macrophages, but was rare in the Golgi apparatus.


CONCLUSIONS
The lack of expression of CD14 on the cell membrane of intestinal macrophages may be caused by transport interference from the endoplasmic reticulum to the Golgi apparatus.
Institute for Health Science, Tokushima Bunri University, Yamashiro-cho, Tokushima-shi, 770-8514, Japan.
Noriko
N
Yoshioka
Yoshie
Y
Taniguchi
Aya
A
Yoshida
Kazue
K
Nakata
Takashi
T
Nishizawa
Hiroyuki
H
Inagawa
Chie
C
Kohchi
Gen-Ichiro
G
Soma
D000818:Animals; D004721:Endoplasmic Reticulum / Q000378:metabolism*; D006056:Golgi Apparatus / Q000378:metabolism*; D007124:Immunoenzyme Techniques; D007422:Intestines / Q000166:cytology; D018950:Lipopolysaccharide Receptors / Q000378:metabolism*; D008264:Macrophages / Q000378:metabolism*; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H
D018950:Lipopolysaccharide Receptors