Migration and actin protrusion in melanoma cells are regulated by EB1 protein.
Remodeling of actin and microtubule cytoskeletons is thought to be coupled; however, the interplay between these two systems is not fully understood. We show a microtubule end-binding protein, EB1, is required for formation of polarize morphology and motility of melanoma cells. EB1 depletion decreased lamellipodia protrusion, and resulted in loss of opposed protruding and retracting cell edges. Lamellipodia attenuation correlated with mis-localization of filopodia throughout the cell and decreased Arp3 localization. EB1-depleted cells displayed less persistent migration and reduced velocity in single-cell motility experiments. We propose EB1 coordinates melanoma cell migration through regulating the balance between lamellipodial and filopodial protrusion.
Schober
Joseph M
JM
Department of Pharmaceutical Sciences, Southern Illinois University School of Pharmacy, 220 University Park Drive, Edwardsville, IL 62026, USA. joschob@siue.edu
Cain
Jeanine M
JM
Komarova
Yulia A
YA
Borisy
Gary G
GG
D000199:Actins / Q000502:physiology*; D000818:Animals; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000502:physiology*; D003599:Cytoskeleton / Q000378:metabolism / Q000648:ultrastructure; D055785:Gene Knockdown Techniques; D008545:Melanoma; D051379:Mice; D008869:Microtubule-Associated Proteins / Q000235:genetics / Q000502:physiology*; D008870:Microtubules / Q000378:metabolism* / Q000648:ultrastructure; D011554:Pseudopodia / Q000502:physiology* / Q000648:ultrastructure
D000199:Actins; C405804:EB1 microtubule binding proteins; D008869:Microtubule-Associated Proteins