Preparation of stable chitosan-carboxymethyl dextran nanoparticles.
Chitosan-carboxymethyl dextran nanoparticles (CHI-CMD NPs) were prepared by the formation of polyelectrolyte complex via the interaction of the positive charged amino group of chitosan and the negative charged carboxyl group of carboxymethyl dextran. Biocompatible organic reagents such as lactic acid, phosphate-buffered saline, and carbonate-bicarbonate buffer were used to make CHI-CMD NPs. The pH value of narrowly distributive CHI-CMD NP suspension was close to the physiological value of 6.8, which made it possible for it to be directly applied in an in vitro test without further additional operations. In the presence of sugars (30 mM), the colloidal stability of CHI-CMD NPs could be extended to 5 to 7 days when the particle size could be maintained within a nanometer scale, while the addition of xylose or lactose was found to most effectively extend the colloidal stability of CHI-CMD NPs to 10 days. Scanning electron microscopy, transmission electron microscopy, Fourier transform infrared spectroscopy, and X-ray diffraction analysis revealed that the structural integrity of CHI-CMD NPs was not destroyed after high steam pressure sterilization (121 degrees C, 30 min), which confirmed the autoclavable property of CHI-CMD NPs.
Lin
Yi-Sheng
YS
Tottori University (United Graduate School, Yamaguchi University), 4-101 Koyama-Minami, Tottori 680-8553, Japan.
Renbutsu
Eiko
E
Morimoto
Minoru
M
Okamura
Yasuhiko
Y
Tsuka
Takeshi
T
Saimoto
Hiroyuki
H
Okamoto
Yoshiharu
Y
Minami
Saburo
S
D048271:Chitosan / Q000138:chemical synthesis* / Q000737:chemistry; D003911:Dextrans / Q000138:chemical synthesis* / Q000737:chemistry; D006358:Hot Temperature; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D036103:Nanotechnology / Q000379:methods; D013242:Sterilization
D003911:Dextrans; D048271:Chitosan; C014392:carboxymethyl dextran