Anti-inflammatory cembranolides from the soft coral Lobophytum durum.
Chemical investigation of the soft coral Lobophytum durum resulted in the isolation of seven new cembranolides, durumolides F-L (1-7), as well as one previously characterized cembranolides, sinularolide D (8). The molecular structures of these isolated metabolites were determined mainly through NMR techniques and HRESIMS analysis. Moreover, the absolute configurations of 1 and 5 were established by application of modified Mosher's method. The antibacterial activities, anti-inflammatory effects, and anti-HCMV (Human cytomegalovirus) endonuclease activity of metabolites 1-8 were also evaluated in vitro. Anti-inflammatory activity of metabolites 1 and 6 (10 microM) significantly reduced the levels of the iNOS protein to 0.8+/-0.6% and 5.7+/-2.2%, respectively, and COX-2 protein to 47.8+/-9.0% and 71.6+/-5.8%, respectively. Metabolites 1-8 (100 microg/disk) exhibited weak antibacterial activity against Salmonella enteritidis.
Cheng
Shi-Yie
SY
Department of Marine Biotechnology and Resources, National Sun Yat-sen University, Kaohsiung 804, Taiwan.
Wen
Zhi-Hong
ZH
Wang
Shang-Kwei
SK
Chiou
Shu-Fen
SF
Hsu
Chi-Hsin
CH
Dai
Chang-Feng
CF
Duh
Chang-Yih
CY
D000818:Animals; D037421:Anthozoa / Q000737:chemistry*; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D000893:Anti-Inflammatory Agents / Q000737:chemistry / Q000494:pharmacology; D002460:Cell Line; D002681:China; D003198:Computer Simulation; D004224:Diterpenes / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D006801:Humans; D008264:Macrophages / Q000187:drug effects*; D009682:Magnetic Resonance Spectroscopy; D051379:Mice; D008958:Models, Molecular; D015394:Molecular Structure; D012477:Salmonella enteritidis / Q000187:drug effects*; D012697:Serine Endopeptidases / Q000378:metabolism
D000900:Anti-Bacterial Agents; D000893:Anti-Inflammatory Agents; D004224:Diterpenes; D012697:Serine Endopeptidases; C071910:assemblin