Bioelectrochemical sensing based on single stranded deoxyribonucleic acid-carbon nanotubes covalently attached on gold electrodes.
A biosensor based on single-stranded deoxyribonucleic acid-functionalized carbon nanotubes covalently attached to a self-assembled monolayer of 11-amino-1-undecanethiol on gold has been prepared. The preparation of the deoxyribonucleic acid sensor was followed using cyclic voltammetry. Single-walled carbon nanotubes, covalently attached to the gold surface present a nanoelectrode array behavior. To confirm the hybridization step between the probe and the target deoxyribonucleic acid, we used methylene blue, which is an indicator capable of distinguishing between single-stranded deoxyribonucleic acid and double-stranded deoxyribonucleic acid. Our results demonstrate that the modification of gold with single-walled carbon nanotubes and single-stranded deoxyribonucleic acid can be used for deoxyribonucleic acid hybridization detection.
Santiago-Rodríguez
Lenibel
L
Department of Chemistry and Center for Nanoscale Materials, University of Puerto Rico, Rio Piedras Campus, P.O. Box 23346, San Juan, Puerto Rico 00931-3346.
Vargas-Barbosa
Nella M
NM
Sánchez-Pomales
Germarie
G
Cabrera
Carlos R
CR
D000473:Alkanes / Q000138:chemical synthesis / Q000737:chemistry; D015374:Biosensing Techniques / Q000379:methods*; D004247:DNA / Q000378:metabolism*; D004277:DNA, Single-Stranded / Q000138:chemical synthesis / Q000737:chemistry / Q000378:metabolism*; D004563:Electrochemistry / Q000379:methods; D004566:Electrodes; D006046:Gold / Q000737:chemistry*; D008751:Methylene Blue / Q000378:metabolism; D037742:Nanotubes, Carbon / Q000737:chemistry*; D009693:Nucleic Acid Hybridization; D013438:Sulfhydryl Compounds / Q000138:chemical synthesis / Q000737:chemistry
C510729:11-amino-1-undecanethiol; D000473:Alkanes; D004277:DNA, Single-Stranded; D037742:Nanotubes, Carbon; D013438:Sulfhydryl Compounds; D006046:Gold; D004247:DNA; D008751:Methylene Blue