Porous polymer/hydroxyapatite scaffolds: characterization and biocompatibility investigations.
Poly-lactic-glycolic acid (PLGA) has been widely used as a scaffold material for bone tissue engineering applications. 3D sponge-like porous scaffolds have previously been generated through a solvent casting and salt leaching technique. In this study, polymer-ceramic composite scaffolds were created by immersing PLGA scaffolds in simulated body fluid, leading to the formation of a hydroxyapatite (HAP) coating. The presence of a HAP layer was confirmed using scanning electron microscopy, energy dispersive X-ray spectroscopy and Fourier transform infrared spectroscopy in attenuated total reflection mode. HAP-coated PLGA scaffolds were tested for their biocompatibility in vitro using human osteoblast cell cultures. Biocompatibility was assessed by standard tests for cell proliferation (MTT, WST), as well as fluorescence microscopy after standard cell vitality staining procedures. It was shown that PLGA-HAP composites support osteoblast growth and vitality, paving the way for applications as bone tissue engineering scaffolds.
Department of Oral and Maxillofacial Surgery, University of Kiel, Arnold-Heller-Strasse 16, 24105, Kiel, Germany. douglas@mkg.uni-kiel.de
Timothy
T
Douglas
Elzbieta
E
Pamula
Dominik
D
Hauk
Jörg
J
Wiltfang
Sureshan
S
Sivananthan
Eugene
E
Sherry
Patrick H
PH
Warnke
D001672:Biocompatible Materials / Q000737:chemistry*; D018786:Bone Substitutes; D001842:Bone and Bones / Q000378:metabolism; D002470:Cell Survival; D002516:Ceramics / Q000737:chemistry; D017886:Durapatite / Q000737:chemistry*; D006801:Humans; D019344:Lactic Acid / Q000737:chemistry*; D008855:Microscopy, Electron, Scanning / Q000379:methods; D010006:Osteoblasts / Q000378:metabolism; D011100:Polyglycolic Acid / Q000737:chemistry*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011108:Polymers / Q000737:chemistry*; D016062:Porosity; D012997:Solvents / Q000737:chemistry; D017550:Spectroscopy, Fourier Transform Infrared; D023822:Tissue Engineering / Q000379:methods
D001672:Biocompatible Materials; D018786:Bone Substitutes; D011108:Polymers; D012997:Solvents; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; D017886:Durapatite