Acquiring local field potential information from amperometric neurochemical recordings.
Simultaneous acquisition of in vivo electrophysiological and neurochemical information is essential for understanding how endogenous neurochemicals modulate the dynamics of brain activity. However, up to now such a task has rarely been accomplished due to the major technical challenge of operating two independent recording systems simultaneously in real-time. Here we propose a simpler solution for achieving this goal by using only a standard electrochemical technique--amperometry. To demonstrate its feasibility, we compared amperometric signals with simultaneously recorded local field potential (LFP) signals. We found that the high frequency component (HFC) of the amperometric signals did not reflect neurochemical fluctuations, but instead it resembled LFPs in several aspects, including: (1) coherent spectral fluctuations; (2) clear characterization of different brain states; (3) identical hippocampal theta depth profile. As such, our findings provide the first demonstration that both LFP and local neurochemical information can be simultaneously acquired from electrochemical sensors alone.
Zhang
Hao
H
Dept. of Neurobiology, Duke University Medical Center, Durham, NC 27710, USA. zhanghao@neuro.duke.edu
Lin
Shih-Chieh
SC
Nicolelis
Miguel A L
MA
D000200:Action Potentials / Q000187:drug effects / Q000502:physiology; D000818:Animals; D015374:Biosensing Techniques / Q000379:methods*; D001921:Brain / Q000187:drug effects / Q000378:metabolism; D001923:Brain Chemistry / Q000187:drug effects / Q000502:physiology*; D018679:Cholinergic Agonists / Q000494:pharmacology; D018491:Dopamine Agonists / Q000494:pharmacology; D004563:Electrochemistry / Q000379:methods*; D004594:Electrophysiology / Q000379:methods*; D006624:Hippocampus / Q000187:drug effects / Q000378:metabolism; D008297:Male; D009448:Neurochemistry / Q000379:methods*; D018377:Neurotransmitter Agents / Q000032:analysis / Q000378:metabolism; D051381:Rats; D020318:Rats, Long-Evans; D012815:Signal Processing, Computer-Assisted; D012890:Sleep / Q000187:drug effects / Q000502:physiology; D013826:Theta Rhythm / Q000187:drug effects
D018679:Cholinergic Agonists; D018491:Dopamine Agonists; D018377:Neurotransmitter Agents