Pharmacokinetics of oral calcitriol in healthy human based on the analysis with an enzyme immunoassay.
We investigated the pharmacokinetics of 2 microg calcitriol in eight healthy human after a single oral administration. Plasma calcitriol was analyzed by sensitive and quantitative enzyme immunoassay following intra- and inter-day validation. In subjects, AUC(0-infinity) and AUC(0-24h) were 267 and 246 pg h ml(-1), respectively. Although the mean plasma concentration of calcitriol in human subjects did not return to baseline at 24h post-dosing, the mean ratio of AUC(0-24h) to AUC(0-infinity) was greater than 80%. The C(max) of calcitriol was measured at 3.4h after administration as 50.0 pg ml(-1). From our results, it can offer new opportunities to design and determine individually appropriate therapeutic dosage regimens based on a pharmacokinetic profile and be used for bioequivalence and reevaluation study of generic drug.
Laboratory of Excellency for Drug and Gene Delivery, College of Pharmacy, Seoul National University, Republic of Korea.
Su-Eon
SE
Jin
Jeong-Sook
JS
Park
Chong-Kook
CK
Kim
D000284:Administration, Oral; D000328:Adult; D019540:Area Under Curve; D001794:Blood Pressure / Q000187:drug effects; D002117:Calcitriol / Q000008:administration & dosage / Q000009:adverse effects / Q000097:blood* / Q000493:pharmacokinetics*; D002118:Calcium / Q000097:blood; D004305:Dose-Response Relationship, Drug; D006339:Heart Rate / Q000187:drug effects; D006801:Humans; D007124:Immunoenzyme Techniques; D010710:Phosphates / Q000097:blood; D015203:Reproducibility of Results; D055815:Young Adult
D010710:Phosphates; D002117:Calcitriol; D002118:Calcium