High cell density fermentation of Gluconobacter oxydans DSM 2003 for glycolic acid production.
Gluconobacter oxydans has a lower biomass yield. Uniform design (UD) was applied to determine the optimum composition of the critical media and their mutual interactions for increased biomass yield of Gluconobacter oxydans DSM 2003 in shake flasks. Fed-batch fermentation process for biomass was optimized in a 3.7-l fermentor. By undertaking a preliminary and improved fed-batch fermentation-process strategy, a cell density of 6.0 g/l (DCW) was achieved in 22 h and 14.1 g/l (DCW) in 35 h, which is the highest cell density of G. oxydans produced thus far in a 3.7-l bioreactor. The biomass production was increased by 135% compared with that using the original cultivation strategy. Bioconversion of ethylene glycol to glycolic acid was catalyzed by the resting cells of G. oxydans DSM 2003, and conversion rate reached 86.7% in 48 h. In summary, the approach including high-density fermentation of G. oxydans DSM 2003 and bioconversion process was established and proved to be an effective method for glycolic acid production.
State Key Lab of Bioreactor Engineering, New World Institute of Biotechnology, East China University of Science and Technology, 200237 Shanghai, China.
Guodong
G
Wei
Xuepeng
X
Yang
Tula
T
Gan
Wenyu
W
Zhou
Jinping
J
Lin
Dongzhi
D
Wei
D018533:Biomass; D019149:Bioreactors; D003470:Culture Media; D019855:Ethylene Glycol / Q000378:metabolism; D005285:Fermentation; D020600:Gluconobacter oxydans / Q000254:growth & development* / Q000378:metabolism*; D006016:Glycolates / Q000378:metabolism*
D003470:Culture Media; D006016:Glycolates; C031149:glycolic acid; D019855:Ethylene Glycol