Reptilian MPR 300 is also the IGF-IIR: cloning, sequencing and functional characterization of the IGF-II binding domain.
The mammalian cation-independent mannose 6-phosphate/insulin-like growth factor (IGF)-II receptor binds IGF-II with high affinity. Ligands transported by the MPR 300/IGF-IIR include IGF-II and mannose 6-phosphate-modified proteins. By targeting IGF-II to lysosomal degradation, it plays a key role in the maintenance of correct IGF-II levels in the circulation and in target tissues. Although, from our studies we found homologous receptor in calotes but its functional significance was not known. We present here the first report on the calotes MPR 300/IGF-IIR binds IGF-II with K(d) of 12.02 nM; these findings provide new and strong evidence that MPR 300/IGF-IIR in Calotes versicolor binds IGFII with high affinity.
Protein Biochemistry and Molecular Biology Laboratory, Department of Biochemistry, University of Hyderabad, Central University Post Office, Gachibowli, Hyderabad 500 046, India.
Yadavalli
Y
Sivaramakrishna
Praveen Kumar
PK
Amancha
Nadimpalli
N
Siva Kumar
D000595:Amino Acid Sequence; D000818:Animals; D003001:Cloning, Molecular; D003432:Cross-Linking Reagents / Q000494:pharmacology; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007335:Insulin-Like Growth Factor II / Q000378:metabolism*; D008024:Ligands; D008099:Liver / Q000166:cytology / Q000378:metabolism; D008969:Molecular Sequence Data; D017434:Protein Structure, Tertiary; D021381:Protein Transport; D017527:Receptor, IGF Type 2 / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism*; D012104:Reptiles / Q000235:genetics; D030162:Reptilian Proteins / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA
D003432:Cross-Linking Reagents; D008024:Ligands; D017527:Receptor, IGF Type 2; D030162:Reptilian Proteins; D007335:Insulin-Like Growth Factor II