Comparative effects of beta-carotene and fucoxanthin on retinol deficiency induced oxidative stress in rats.
This study aimed at comparing antioxidant potential of fucoxanthin (FUCO) with beta-carotene in relieving lipid peroxidation (Lpx) caused by retinol deficiency (RD) in rats. RD rats (n = 45) were fed a dose of either beta-carotene (0.81 mumol) or FUCO (0.83 mumol). Plasma and liver lipid peroxide levels and activity of antioxidant enzymes catalase (CAT) and glutathione transferase (GST) were measured for 8 h. Results revealed that RD increased (P < 0.05) Lpx in plasma and liver by 34.3% and 19.4%, while the CAT activity in plasma (89%) and liver microsomes (91%) and GST in liver homogenate (31%) and liver microsomes (30%) were decreased (P < 0.05) compared to control (rats fed basal diet). FUCO suppressed (P < 0.05) the Lpx level by 7-85% (plasma) and 24-72% (liver) as compared to beta-carotene (51-76%, 33-65%) over a period of 8 h. The activity of CAT in plasma and liver microsomes was higher (P < 0.05) in FUCO (90-95%, 85-93%) and beta-carotene (87-96%, 79-91%) groups as compared to RD group. Similarly, the activity of GST in liver and its microsomes was also elevated (P < 0.05) in FUCO (44-51%, 22-51%) and beta-carotene (19-54%, 30-43%) groups as compared to RD group. Results demonstrate that FUCO has greater potential than beta-carotene in modulating Lpx, CAT, GST in plasma and liver of RD rats.
Department of Biochemistry & Nutrition, Central Food Technological Research Institute, CSIR, Mysore, Karnataka, 570 020, India.
R K
RK
Sangeetha
N
N
Bhaskar
V
V
Baskaran
D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D002374:Catalase / Q000097:blood; D002851:Chromatography, High Pressure Liquid; D005227:Fatty Acids / Q000097:blood; D005982:Glutathione Transferase / Q000097:blood; D015227:Lipid Peroxidation / Q000187:drug effects; D008862:Microsomes, Liver / Q000187:drug effects / Q000201:enzymology; D018384:Oxidative Stress / Q000187:drug effects*; D051381:Rats; D017208:Rats, Wistar; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism; D013056:Spectrophotometry, Ultraviolet; D013997:Time Factors; D014801:Vitamin A / Q000097:blood; D014802:Vitamin A Deficiency / Q000188:drug therapy*; D024341:Xanthophylls / Q000008:administration & dosage / Q000097:blood / Q000494:pharmacology* / Q000627:therapeutic use*; D019207:beta Carotene / Q000008:administration & dosage / Q000378:metabolism / Q000494:pharmacology* / Q000627:therapeutic use*
D000975:Antioxidants; D005227:Fatty Acids; D024341:Xanthophylls; D019207:beta Carotene; C025164:fucoxanthin; D014801:Vitamin A; D002374:Catalase; D005982:Glutathione Transferase; D000254:Sodium-Potassium-Exchanging ATPase