Studies on the interaction between benzidine and hemocyanin from Chinese mitten crab Eriocheir japonica sinensis (Decapoda, Grapsidae).
The interaction of benzidine (BZ) with hemocyanin (Hc) from Chinese mitten crab (Eriocheir japonica sinensis) was studied by fluorescence spectrum. BZ can quench the fluorescence of Hc by forming a new complex. The process of binding BZ on Hc was a spontaneous molecular interaction procedure. The thermodynamic parameters, the enthalpy change and entropy change were estimated to be 47.36 kJ mol(-1), 248.51 J mol(-1) K(-1) according to the van' Hoff equation. This indicates that hydrophobic interaction played a major role in stabilizing the complex. The results of synchronous fluorescence spectroscopy and three-dimensional fluorescence spectra indicated that the structure of these tyrosine residues environments was altered by BZ, which could enter into the hydrophobic pocked of Hc.
Jiangsu Provincial Key Laboratory of Coastal Wetland Bioresources and Environmental Protection, Yancheng Teachers University, Kaifang Dadao Road 50, Yancheng City, Jiangsu Province 224002, People's Republic of China. tangwang2008@163.com
Bo-Ping
BP
Tang
Yan-Qing
YQ
Wang
Dai-Zhen
DZ
Zhang
D000818:Animals; D001560:Benzidines / Q000494:pharmacology*; D003386:Brachyura / Q000378:metabolism*; D006433:Hemocyanins / Q000737:chemistry / Q000378:metabolism*; D011485:Protein Binding; D011487:Protein Conformation; D013050:Spectrometry, Fluorescence; D013816:Thermodynamics
D001560:Benzidines; C029876:benzidine; D006433:Hemocyanins