Effect of lidocaine hydrochloride on the function of bovine peripheral leukocytes.
To clarify the effect of lidocaine hydrochloride (Lid) on bovine peripheral granulocyte phagocytosis, adhesion molecule expression of leukocytes and peripheral blood mononuclear cell mRNA expression of cytokines were investigated. Lid was added to blood samples at a final concentration of 0 (only PBS; Cont), 0.2 mg/ml or 2.0 mg/ml. Phagocytosis of granulocytes was significantly decreased by addition of 2.0 mg/ml of Lid. CD18 expression of granulocytes and mononuclear cells were significantly reduced by addition of 2.0 mg/ml of Lid. IL-1beta and IL-8 mRNA expressions of mononuclear cells were also significantly reduced by addition of 2.0 mg/ml of Lid other hand. These results suggest that Lid might reduce the protective immunity of cows. On the other hand, reduction of CD18, IL-1beta and IL-8 mRNA expression also indicates that Lid has an anti-inflammatory effect in cows.
School of Veterinary Medicine, Rakuno Gakuen University, Japan. ando@rakuno.ac.jp
Takaaki
T
Ando
Jin
J
Ogawa
Hiroki
H
Fujiwara
Satoshi
S
Yokotachi
Kenichi
K
Maeda
Masayuki
M
Kohiruimaki
Hiromichi
H
Ohtsuka
Daisaku
D
Watanabe
D000779:Anesthetics, Local / Q000494:pharmacology*; D000818:Animals; D018821:CD18 Antigens / Q000096:biosynthesis / Q000097:blood / Q000276:immunology; D002417:Cattle / Q000097:blood* / Q000276:immunology; D016207:Cytokines / Q000235:genetics / Q000276:immunology; D005260:Female; D005434:Flow Cytometry / Q000662:veterinary; D006098:Granulocytes / Q000187:drug effects / Q000276:immunology; D053583:Interleukin-1beta / Q000096:biosynthesis / Q000235:genetics; D016209:Interleukin-8 / Q000096:biosynthesis / Q000235:genetics; D007958:Leukocyte Count / Q000662:veterinary; D007963:Leukocytes, Mononuclear / Q000187:drug effects* / Q000276:immunology; D008012:Lidocaine / Q000494:pharmacology*; D010587:Phagocytosis / Q000187:drug effects / Q000276:immunology; D012333:RNA, Messenger / Q000096:biosynthesis / Q000097:blood / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis / Q000235:genetics
D000779:Anesthetics, Local; D018821:CD18 Antigens; D016207:Cytokines; D053583:Interleukin-1beta; D016209:Interleukin-8; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha; D008012:Lidocaine