The engineering of bacteria bearing azido-pseudaminic acid-modified flagella.
Catch a tiger by the tail: We have demonstrated that by feeding nonmotile mutant C. jejuni bacteria with a neutral azide-labelled pseudaminic acid precursor we can restore their ability to generate functional flagella. The presence of azido-pseudaminic acid on the surface of the flagella provides a bio-orthogonal chemical handle that can be used to modify the flagellar proteins.
Department of Chemistry, The University of British Columbia, 2036 Main Mall, Vancouver, B.C. V6T 1Z1, Canada.
Feng
F
Liu
Annie J
AJ
Aubry
Ian C
IC
Schoenhofen
Susan M
SM
Logan
Martin E
ME
Tanner
D001386:Azides / Q000737:chemistry*; D016123:Campylobacter jejuni / Q000737:chemistry*; D005408:Flagellin / Q000737:chemistry*; D015202:Protein Engineering; D013194:Staining and Labeling; D013400:Sugar Acids / Q000737:chemistry / Q000378:metabolism*
C513444:5,7-diacetamido-3,5,7,9-tetradeoxynonulosonic acid; D001386:Azides; D013400:Sugar Acids; D005408:Flagellin