Tandem organization of StarkB element (22.8 kb) in the maize B chromosome.
A very large repetitive element (StarkB, 22.8 kb) is present in the maize B chromosome, presumably not organized as tandem arrays. Results of the current study are contrary to this notion. Out of eighteen StarkB-carrying sequences, nine were the expected internal fragment of StarkB, and nine others were fragments spanning two StarkB elements. One of the two StarkB components, GrandeB, was flanked in all clones with identical target sequences, as opposed to other Grandes that are associated with different target sequences. Also observed was a prominent Southern signal associated with a fragment representing the junction of two adjacent StarkB units. A clone possessing a structure inverse to that of the second component of StarkB is proposed to be the initial element into which a GrandeB inserted to derive StarkB. Most, if not all, isolated StarkB arrays were not the original form, being disrupted by the invasion of various mobile elements intertwined with various stages of amplification.
Lo
Kuan-lin
KL
Institute of Molecular Biology, National Chung Hsing University, Taiwan, ROC.
Peng
Shu-fen
SF
Chen
Liang-jwu
LJ
Lin
Bor-yaw
BY
D001483:Base Sequence; D015139:Blotting, Southern; D032461:Chromosomes, Plant / Q000235:genetics*; D008969:Molecular Sequence Data; D018626:Retroelements / Q000235:genetics; D020080:Tandem Repeat Sequences / Q000235:genetics*; D003313:Zea mays / Q000235:genetics*
D018626:Retroelements