Location and properties of respiratory neurones with putative intrinsic bursting properties in the rat in situ.
Using the in situ arterially perfused preparations of both neonatal and juvenile rats, we provide the first description of the location, morphology and transmitter content of a population of respiratory neurones that retains a bursting behaviour after ionotropic receptor blockade. All burster neurones exhibited an inspiratory discharge during eupnoeic respiration. These neurones were predominantly glutamatergic, and were located within a region of the ventral respiratory column that encompasses the pre-Bötzinger complex and the more caudally located ventral respiratory group. Bursting behaviour was both voltage and persistent sodium current dependent and could be stimulated by sodium cyanide to activate this persistent sodium current. The population of burster neurones may overlap with that previously described in the neonatal slice in vitro. Based upon the present and previous findings, we hypothesize that this burster discharge may be released when the brain is subject to severe hypoxia or ischaemia, and that this burster discharge could underlie gasping.
Department of Physiology, Dartmouth Medical School, Dartmouth-Hitchcock Medical Center, Lebanon, NH 03755, USA. Walter.M.StJohn@Dartmouth.edu
Walter M
WM
St-John
Ruth L
RL
Stornetta
Patrice G
PG
Guyenet
Julian F R
JF
Paton
D000818:Animals; D000831:Animals, Newborn; D055724:Electrophysiological Phenomena; D020925:Hypoxia-Ischemia, Brain / Q000503:physiopathology; D008526:Medulla Oblongata / Q000166:cytology* / Q000502:physiology*; D008564:Membrane Potentials; D018408:Patch-Clamp Techniques; D051381:Rats; D012119:Respiration; D012964:Sodium / Q000378:metabolism; D009435:Synaptic Transmission
D012964:Sodium