Dietary carbohydrate level affects transcription factor expression that regulates skeletal muscle myogenesis in rainbow trout.
Understanding the effects of dietary carbohydrates on transcription factors that regulate myogenesis provides insight into the role of nutrient sensing by satellite cells towards myocyte differentiation. We evaluated the influence of dietary carbohydrate level (0, 15, 25 or 35%) on the temporal mRNA expression patterns (4, 8 or 12 weeks) of transcription factors that regulate satellite cell myocyte addition (MA) in rainbow trout (Oncorhynchus mykiss), a vertebrate with indeterminate growth. Relative to the 0% carbohydrate (NC) diet, 15 (IC-15) and 25% (IC-25) carbohydrate containing diets significantly up-regulate MyoD and Myf5, but not Pax7, after 12 weeks of feeding. Simultaneously, the Pax7/MyoD mRNA expression ratio declined significantly with both the IC diets. Myogenin mRNA expression also increased in rainbow trout (RBT) fed the IC-15 diet. The high carbohydrate (HC) diet (35%) attenuated the increased mRNA expression of these transcription factors. It is of note that the 4 and 8 week samples lacked the promyogenic expression patterns. The myogenic gene expression in fish fed the IC-15 diet for 12 weeks indicate a transcriptional signature that reflects increased satellite cell myogenesis. Our results suggest a potential role for satellite cells in the nutrient sensing ability of a vertebrate with indeterminate skeletal muscle growth.
Department of Animal and Veterinary Science, University of Idaho, Moscow, Idaho 83844, USA.
Kalyan C
KC
Chapalamadugu
Barrie D
BD
Robison
Robert E
RE
Drew
Madison S
MS
Powell
Rodney A
RA
Hill
Jon J
JJ
Amberg
Kenneth J
KJ
Rodnick
Ronald W
RW
Hardy
Madison L
ML
Hill
Gordon K
GK
Murdoch
D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects / Q000235:genetics; D004040:Dietary Carbohydrates / Q000008:administration & dosage*; D029941:Fish Proteins / Q000235:genetics*; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000187:drug effects; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects; D032342:Muscle Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D024510:Muscle Development / Q000235:genetics; D018482:Muscle, Skeletal / Q000166:cytology / Q000254:growth & development / Q000378:metabolism*; D017570:MyoD Protein / Q000235:genetics; D051787:Myogenic Regulatory Factor 5 / Q000235:genetics; D017686:Oncorhynchus mykiss / Q000235:genetics* / Q000254:growth & development; D051763:PAX7 Transcription Factor / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D032496:Satellite Cells, Skeletal Muscle / Q000378:metabolism; D013997:Time Factors; D014157:Transcription Factors / Q000235:genetics*
D004040:Dietary Carbohydrates; D029941:Fish Proteins; D017570:MyoD Protein; D051787:Myogenic Regulatory Factor 5; D051763:PAX7 Transcription Factor; D014157:Transcription Factors