Letter: Collision energy and cone voltage optimisation for glycopeptide analysis.
Instrument tuning commonly used for peptide analysis and for proteomics causes a high degree of fragmentation for glycopeptides. This results in a strongly biased glycosylation pattern. To obtain correct results for glycopeptides, both the cone voltage and the collision energy has to be reduced significantly. A suitable standard for tuning the instrument for glycopeptide analysis is aspartic acid (which fragments under similar conditions as glycopeptides); while low mass sugar fragments (for example, at 657.3 Da) are good indicators for the presence/absence of glycopeptide fragmentation.
Krenyacz
Judit
J
Drahos
László
L
Vékey
Károly
K
D001224:Aspartic Acid / Q000737:chemistry; D006020:Glycopeptides / Q000032:analysis*; D006031:Glycosylation; D013058:Mass Spectrometry / Q000379:methods*; D040901:Proteomics
D006020:Glycopeptides; D001224:Aspartic Acid