Comparative analysis of DNA methylation profiles in peripheral blood leukocytes versus lymphoblastoid cell lines.
Previous reports have shown that DNA methylation profiles within primary human malignant tissues are altered when these cells are transformed into cancer cell lines. However, it is unclear if similar differences in DNA methylation profiles exist between DNA derived from peripheral blood leukocytes (PBLs) and corresponding Epstein-Barr Virus transformed lymphoblastoid cell lines (LCLs). To assess the utility of LCLs as a resource for methylation studies we have compared DNA methylation profiles in promoter and 5' regions of 318 genes in PBL and LCL sample pairs from patients with type 1 diabetes with or without nephropathy. We identified a total of 27 (approximately 8%) genes that revealed different DNA methylation profiles in PBL compared with LCL-derived DNA samples. In conclusion, although the profiles for most promoter regions were similar between PBL-LCL pairs, our results indicate that LCL-derived DNA may not be suitable for DNA methylation studies at least in diabetic nephropathy.
Nephrology Research Group, Queen's University Belfast, Belfast, Northern, Ireland.
Eoin P
EP
Brennan
Mathias
M
Ehrich
Derek P
DP
Brazil
John K
JK
Crean
Madeline
M
Murphy
Denise M
DM
Sadlier
Finian
F
Martin
Catherine
C
Godson
Amy Jayne
AJ
McKnight
Dirk
D
van den Boom
Alexander P
AP
Maxwell
David A
DA
Savage
D002461:Cell Line, Transformed; D002472:Cell Transformation, Viral / Q000235:genetics*; D019175:DNA Methylation; D044127:Epigenesis, Genetic; D020031:Epstein-Barr Virus Infections / Q000235:genetics*; D020869:Gene Expression Profiling; D015973:Gene Expression Regulation, Leukemic; D006801:Humans; D007962:Leukocytes / Q000378:metabolism*; D008223:Lymphoma / Q000235:genetics* / Q000821:virology; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization