Prays oleae midgut putative receptor of Bacillus thuringiensis vegetative insecticidal protein Vip3LB differs from that of Cry1Ac toxin.
Vegetative insecticidal protein (Vip) is a class of insecticidal proteins produced by many Bacillus thuringiensis strains during their vegetative growth stage. The vip3LB gene of B. thuringiensis strain BUPM95, which encodes a protein active against the Lepidoptera olive tree pathogenic insect Prays oleae, was cloned into pET-14b vector and overexpressed in Escherichia coli. The expressed Vip3LB protein, found in the E. coli cytoplasmic fraction, was purified and used to produce anti-Vip3LB antibodies. Using the midgut extract of P. oleae, the purified Vip3LB bound to a 65-kDa protein, whereas Cry1Ac toxin bound to a 210-kDa midgut putative receptor. This result justifies the importance of the biological pest control agent Vip3LB that could be used as another alternative particularly in case of resistance to Cry toxins.
Laboratory of Biopesticides, Centre of Biotechnology of Sfax, P.O. Box 1177, 3018 Sfax, Tunisia.
Lobna
L
Abdelkefi-Mesrati
Souad
S
Rouis
Sameh
S
Sellami
Samir
S
Jaoua
D000818:Animals; D001323:Autoantibodies / Q000276:immunology*; D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins / Q000235:genetics / Q000276:immunology* / Q000378:metabolism*; D004731:Endotoxins / Q000235:genetics / Q000276:immunology*; D006460:Hemolysin Proteins / Q000235:genetics / Q000276:immunology*; D007422:Intestines / Q000276:immunology*; D007915:Lepidoptera / Q000276:immunology*; D010572:Pest Control, Biological / Q000379:methods*; D015202:Protein Engineering
D001323:Autoantibodies; D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D004731:Endotoxins; D006460:Hemolysin Proteins; C447139:Vip3A protein, Bacillus thuringiensis; C030956:insecticidal crystal protein, Bacillus Thuringiensis