Functional analysis of MycE and MycF, two O-methyltransferases involved in the biosynthesis of mycinamicin macrolide antibiotics.
Mg motors: We characterized the in vitro function of MycE and MycF, two O-methyltransferases involved in the biosynthesis of mycinamicin antibiotics. Each enzyme was confirmed to be an S-adenosyl-L-methionine (SAM)-dependent deoxysugar methyltransferase. Their optimal activities require the presence of Mg(2+). With the reconstituted in vitro assays, the order of mycinamicin VI-->III-->IV in the post-PKS (polyketide synthase) tailoring pathway of mycinamicin was unambiguously determined.
Li
Shengying
S
Life Sciences Institute, Department of Medicinal Chemistry, Chemistry, and Microbiology and Immunology, University of Michigan, 210 Washtenaw Avenue, Ann Arbor, MI 48109-2216, USA.
Anzai
Yojiro
Y
Kinoshita
Kenji
K
Kato
Fumio
F
Sherman
David H
DH
D000595:Amino Acid Sequence; D000900:Anti-Bacterial Agents / Q000096:biosynthesis* / Q000737:chemistry; D007700:Kinetics; D018942:Macrolides / Q000737:chemistry*; D008274:Magnesium / Q000378:metabolism; D008780:Methyltransferases / Q000378:metabolism; D008969:Molecular Sequence Data; D005810:Multigene Family; D010802:Phylogeny; D048630:Polyketide Synthases / Q000378:metabolism; D011497:Protein O-Methyltransferase / Q000737:chemistry* / Q000145:classification / Q000378:metabolism; D017386:Sequence Homology, Amino Acid
D000900:Anti-Bacterial Agents; D018942:Macrolides; C026686:mycinamicins; D048630:Polyketide Synthases; D008780:Methyltransferases; D011497:Protein O-Methyltransferase; C483301:S-adenosyl-L-methionine (R,S)-reticuline 7-O-methyltransferase; D008274:Magnesium