Discovery of novel plant peptides as strong inhibitors of metalloproteinases.
Five novel metalloproteinase protein inhibitors (MPIs) with molecular mass between 5.6 and 8.9 kDa and acid/neutral pI were detected in lupin seeds and exhibited strong inhibitory activities against thermolysin and/or gelatinase B. These novel peptides constitute not only the first MPIs described in plants but also the first plant peptides with inhibitory activity against a matrixin.
Instituto Superior de Agronomia, Universidade Técnica de Lisboa, Departamento de Botânica e Engenharia Biológica, 1349-017 Lisbon, Portugal. carrilho@itqb.unl.pt
Dina M
DM
Carrilho
Isabel C
IC
Duarte
Rita
R
Francisco
Cândido P P
CP
Ricardo
Maria C
MC
Duque-Magalhães
D000818:Animals; D001419:Bacteria / Q000201:enzymology; D019295:Computational Biology; D030562:Databases, Protein; D055808:Drug Discovery; D015180:Electrophoresis, Gel, Two-Dimensional; D006801:Humans; D031297:Lupinus / Q000737:chemistry*; D045726:Metalloproteases / Q000037:antagonists & inhibitors*; D008970:Molecular Weight; D010455:Peptides / Q000032:analysis / Q000737:chemistry / Q000494:pharmacology*; D010936:Plant Extracts / Q000032:analysis / Q000737:chemistry / Q000494:pharmacology; D010940:Plant Proteins / Q000032:analysis / Q000737:chemistry / Q000494:pharmacology*; D011480:Protease Inhibitors / Q000032:analysis / Q000737:chemistry / Q000494:pharmacology*; D040901:Proteomics; D012639:Seeds / Q000737:chemistry; D053719:Tandem Mass Spectrometry; D013820:Thermolysin / Q000037:antagonists & inhibitors; D014462:Ultrafiltration
D010455:Peptides; D010936:Plant Extracts; D010940:Plant Proteins; D011480:Protease Inhibitors; D045726:Metalloproteases; D013820:Thermolysin