Chemotherapy induced transient B-cell depletion boosts antibody-forming cells expansion driven by an epidermal growth factor-based cancer vaccine.
Cancer vaccines efficacy may improve inducing a rapid and persistent immune response, early at diagnosis along with standard therapies. EGF chemically conjugated to the carrier protein P64k from Neisseria meningitidis in montanide ISA 51 adjuvant is under evaluation, aiming to stimulate a B-cell response. High-dose cyclophosphamide and doxorubicin after priming enhanced the long-term frequency of EGF-specific antibody-forming cells (AFC) of IgM and IgG isotypes, but not the P64k response. Resulting combination, limitedly operational in Btk deficient xid mice, suggests that preferential B-cell lymphocyte space promoted by cyclophosphamide facilitates remaining EGF-specific AFC undergo homeostatic proliferation driven by boosting, amplifying the response.
Experimental Immunotherapy Department, Center of Molecular Immunology, 216 Street & 15, Playa, Havana 11600, Cuba. enrique@cim.sld.cu
Enrique
E
Montero
Maikel
M
Valdes
Janet
J
Avellanet
Armando
A
Lopez
Rolando
R
Perez
Agustin
A
Lage
D000818:Animals; D000921:Antibody-Producing Cells / Q000502:physiology*; D001402:B-Lymphocytes / Q000187:drug effects*; D001425:Bacterial Outer Membrane Proteins / Q000008:administration & dosage / Q000276:immunology; D019496:Cancer Vaccines / Q000276:immunology*; D049109:Cell Proliferation; D003520:Cyclophosphamide / Q000494:pharmacology*; D004317:Doxorubicin / Q000494:pharmacology*; D004815:Epidermal Growth Factor / Q000276:immunology*; D005260:Female; D007074:Immunoglobulin G / Q000096:biosynthesis; D007075:Immunoglobulin M / Q000096:biosynthesis; D008212:Lymphocyte Depletion; D008353:Mannitol / Q000008:administration & dosage / Q000031:analogs & derivatives; D051379:Mice; D008807:Mice, Inbred BALB C; D009829:Oleic Acids / Q000008:administration & dosage
D001425:Bacterial Outer Membrane Proteins; D019496:Cancer Vaccines; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D009829:Oleic Acids; C112623:lpdA protein, Neisseria meningitidis; C477385:montanide ISA 51; D008353:Mannitol; D004815:Epidermal Growth Factor; D004317:Doxorubicin; D003520:Cyclophosphamide