A combination of thalidomide and augmentin protects BALB/c mice suffering from Klebsiella pneumoniae B5055-induced sepsis.
Despite extensive research, the mortality associated with sepsis in hospitals remains very high. We have evaluated the protective immunomodulatory effect of thalidomide alone or with Augmentin in Klebsiella pneumoniae B5055-induced sepsis in BALB/c mice. The mouse model of sepsis was developed by placing K. pneumoniae B5055 entrapped in fibrin and thrombin clots in the peritoneal cavity of mice. The septic mice were treated with thalidomide alone (30 mg/kg/day/po), Augmentin alone (20 microg/ml/ip) and with their combination. the thalidomide-alone treated mice showed 75% survival whereas 60% of the Augmentin-alone treated group survived. Combination treatment provided 100% survival. Treatment with thalidomide alone significantly (p<0.05) decreased interleukin-1 alpha (IL-1alpha), nitric oxide (NO) and malondialdehyde (MDA) levels in the serum without significantly (p<0.05) decreasing the bacterial count in blood. Augmentin alone only decreased the bacterial load in blood significantly (p<0.05). However, a combination of thalidomide with Augmentin significantly (p<0.05) decreased both the bacterial count and inflammatory mediators.
Department of Microbiology, Panjab University, Chandigarh, India. vij_tox@yahoo.com
V
V
Kumar
K
K
Harjai
S
S
Chhibber
D019980:Amoxicillin-Potassium Clavulanate Combination / Q000008:administration & dosage*; D000818:Animals; D000900:Anti-Bacterial Agents / Q000008:administration & dosage*; D004359:Drug Therapy, Combination; D004797:Enzyme-Linked Immunosorbent Assay; D007166:Immunosuppressive Agents / Q000008:administration & dosage*; D053582:Interleukin-1alpha / Q000097:blood; D007710:Klebsiella Infections / Q000188:drug therapy*; D007711:Klebsiella pneumoniae; D008315:Malondialdehyde / Q000097:blood; D051379:Mice; D008807:Mice, Inbred BALB C; D009569:Nitric Oxide / Q000097:blood; D018805:Sepsis / Q000188:drug therapy* / Q000382:microbiology; D013792:Thalidomide / Q000008:administration & dosage*
D000900:Anti-Bacterial Agents; D007166:Immunosuppressive Agents; D053582:Interleukin-1alpha; D009569:Nitric Oxide; D008315:Malondialdehyde; D013792:Thalidomide; D019980:Amoxicillin-Potassium Clavulanate Combination