Mechanisms of stress resistance in Snell dwarf mouse fibroblasts: enhanced antioxidant and DNA base excision repair capacity, but no differences in mitochondrial metabolism.
Dermal fibroblasts from long-lived Snell dwarf mice can withstand a variety of oxidative and non-oxidative stressors compared to normal littermate controls. Here, we report differences in the levels and activities of intracellular antioxidant and DNA repair enzymes between normal and Snell dwarf mice fibroblasts cultured under a variety of conditions, including: 3% and 20% ambient O(2); the presence and absence of serum; and the addition of an exogenous oxidative stress. The only significant difference between normal and dwarf cells cultured in complete medium, at 20% O(2), was an approximately 40% elevation of glutathione peroxidase (GPx) activity in the mutant cells. Serum deprivation elicited increases in GPx in both genotypes, but these activities remained higher in dwarf mouse cells. Dwarf mouse cells deprived of serum and challenged with exposure to paraquat or hydrogen peroxide showed a generally greater upregulation of catalase and DNA base excision repair enzymes. As these toxins can interact with mitochondria to increase mitochondrial ROS production, we explored whether there were differences in mitochondrial metabolism between normal and dwarf mouse cells. However, neither mitochondrial content nor the apparent mitochondrial membrane potential differed between genotypes. Overall, the results suggest that superior hydrogen peroxide metabolism and a marginally greater DNA base excision repair capacity contribute to the stress resistance phenotype of Snell dwarf mouse fibroblasts.
Department of Biological Sciences, Brock University, St Catharines, Ontario, Canada.
Melissa M
MM
Page
Adam B
AB
Salmon
Scott F
SF
Leiser
Ellen L
EL
Robb
Melanie F
MF
Brown
Richard A
RA
Miller
Jeffrey A
JA
Stuart
D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D016895:Culture Media, Serum-Free / Q000378:metabolism; D004247:DNA / Q000032:analysis*; D004260:DNA Repair / Q000502:physiology*; D004789:Enzyme Activation / Q000502:physiology; D005347:Fibroblasts / Q000201:enzymology* / Q000648:ultrastructure; D005979:Glutathione Peroxidase / Q000378:metabolism*; D006861:Hydrogen Peroxide / Q000378:metabolism; D053078:Membrane Potential, Mitochondrial / Q000502:physiology; D051379:Mice; D008817:Mice, Mutant Strains; D008928:Mitochondria / Q000378:metabolism*; D018384:Oxidative Stress / Q000502:physiology
D000975:Antioxidants; D016895:Culture Media, Serum-Free; D004247:DNA; D006861:Hydrogen Peroxide; D005979:Glutathione Peroxidase