The effect of wasabi rhizome extract on atopic dermatitis-like symptoms in HR-1 hairless mice.
We investigated the effect of wasabi rhizome extract on atopic dermatitis (AD) model mice. The wasabi extract was fed to the HR-1 hairless mice, which develop AD-like symptoms with a special diet (HR-AD diet). The extract was expected to reduce the symptoms induced. Wasabi rhizome-containing HR-AD diet (5% and 10%) reduced the scratching behavior, and the 10% wasabi rhizome HR-AD diet significantly reduced scratching behavior on days 28, 35 and 42. Plasma components (histamine, eotaxin, IgE and thymus and activation-regulated chemokine (TARC)) were decreased in the 10% wasabi rhizome HR-AD diet. In histopathological examinations (toluidine blue (T.B.), major basic protein (MBP), CD4, IL-4, IL-5, eotaxin, TARC and IgE), the wasabi rhizome-containing HR-AD diet (5% and 10%) significantly reduced the number of positive stained cells. These results suggested that the wasabi rhizome extract improved the AD-like symptoms of HR-1 hairless mice.
Nagai
Masashi
M
Kinjirushi Co., Ltd., Nagoya, Japan. m-nagai@kinjirushi.co.jp
Okunishi
Isao
I
D000818:Animals; D000906:Antibodies / Q000032:analysis; D001522:Behavior, Animal / Q000187:drug effects; D019607:Brassicaceae; D054414:Chemokine CCL17 / Q000097:blood; D019742:Chemokines, CC / Q000097:blood; D003876:Dermatitis, Atopic / Q000188:drug therapy*; D004195:Disease Models, Animal; D002149:Energy Intake / Q000187:drug effects; D006632:Histamine / Q000097:blood; D007073:Immunoglobulin E / Q000097:blood; D008297:Male; D051379:Mice; D008812:Mice, Hairless; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology / Q000627:therapeutic use*; D011537:Pruritus / Q000188:drug therapy; D027343:Rhizome; D012867:Skin / Q000276:immunology*; D015430:Weight Gain / Q000187:drug effects
D000906:Antibodies; D054414:Chemokine CCL17; D019742:Chemokines, CC; D010936:Plant Extracts; D007073:Immunoglobulin E; D006632:Histamine