Cytological characterization of Vicia oroboides Wulfen in Jacq.
Vicia oroboides, a rare taxon belonging to section Atossa of subgenus Vicia, was recovered and analysed by means of cytological and karyological methods with the aim of both characterising this species and integrating our knowledge on phylogeny of subgenus Vicia. Automated karyotype analysis and nuclear DNA content have been determined after Feulgen's reaction; chromosome banding was performed by fluorochrome staining to evidence heterochromatic blocks along the chromosome complement. The chromosome number is in line with the values of the species of section Atossa; the GC- and AT-rich sites were identified by CMA and DAPI staining. Karyomorphological parameters, based on symmetry indices, provide information about the phylogenetic position of this species inside the subgenus Vicia. DNA content is reported for the first time.
Dipartimento di Biologia, Università di Pisa, Via L. Ghini 5, 56126, Pisa, Italy. mruffini@biologia.unipi.it
Monica
M
Ruffini Castiglione
M
M
Frediani
C
C
Ravalli
G
G
Venora
R
R
Cremonini
D002871:Chromosome Banding; D032461:Chromosomes, Plant / Q000235:genetics*; D018744:DNA, Plant / Q000235:genetics*; D019143:Evolution, Molecular; D007621:Karyotyping; D031306:Vicia / Q000166:cytology* / Q000235:genetics*
D018744:DNA, Plant