Nanoroughened plasmonic films for enhanced biosensing detection.
Although fluorescence is the prevailing labeling technique in biosensing applications, sensitivity improvement is still a striving challenge. We show that coating standard microscope slides with nanoroughened silver films provides a high fluorescence signal enhancement due to plasmonic interactions. As a proof of concept, we applied these films with tailored plasmonic properties to DNA microarrays. Using common optical scanning devices, we achieved signal amplifications of more than 40-fold.
Institut Langevin, ESPCI ParisTech, Université Paris Diderot, CNRS UMR 7587, 10 rue Vauquelin, F-75231 Paris Cedex 05, France.
Eric
E
Le Moal
Sandrine
S
Lévêque-Fort
Marie-Claude
MC
Potier
Emmanuel
E
Fort
D015374:Biosensing Techniques / Q000379:methods*; D002232:Carbocyanines / Q000737:chemistry; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000737:chemistry; D018625:Microscopy, Atomic Force; D036103:Nanotechnology / Q000379:methods*; D016011:Normal Distribution; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D012235:Rhodamines / Q000737:chemistry; D012680:Sensitivity and Specificity; D012834:Silver / Q000737:chemistry; D020349:Surface Plasmon Resonance; D013499:Surface Properties
D002232:Carbocyanines; D005456:Fluorescent Dyes; D012235:Rhodamines; C089187:cyanine dye 3; D012834:Silver; C029773:rhodamine B