Immunohistochemical detection of advanced glycation end  products in human bladder with specific monoclonal antibody.
OBJECTIVE
To investigate the accumulation of advanced glycation end products (AGE) in human bladder.


METHODS
Human bladder specimens were obtained from nine patients during radical cystectomy. Frozen sections were immunohistochemically analyzed by three different monoclonal anti-AGE antibodies such as anti-N(epsilon)-(carboxymethyl)lysine (CML), anti-imidazolone and anti-pentosidine antibodies. Bladder sections were stained with these antibodies by indirect immunoperoxidase methods. Double immunohistochemical staining with one of the anti-AGE antibodies or an anti-human macrophage antibody was also carried out.


RESULTS
We demonstrated that CML and pentosidine were accumulated in human bladder extracellularly as well as intracellularly, whereas any accumulation of imidazolone was not observed. Double immunohistochemical staining indicated that AGE-accumulated cells in human bladder were derived from macrophages.


CONCLUSIONS
The present study demonstrated that AGE-structures such as CML and pentosidine are accumulated extracellularly in human bladder, and were endocytosed by tissue macrophages.
Department of Urology, Kumamoto University Graduate School of Medicine, Kumamoto, Japan. kenshilund@hotmail.com
Kenshi
K
Matsumoto
Yukio
Y
Fujiwara
Ryoji
R
Nagai
Masaki
M
Yoshida
D000368:Aged; D000911:Antibodies, Monoclonal / Q000032:analysis*; D005260:Female; D017127:Glycation End Products, Advanced / Q000032:analysis* / Q000276:immunology*; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D001743:Urinary Bladder / Q000737:chemistry*
D000911:Antibodies, Monoclonal; D017127:Glycation End Products, Advanced