A chemoenzymatic route to N-acetylglucosamine-1-phosphate analogues: substrate specificity investigations of N-acetylhexosamine 1-kinase.
Reports an efficient chemoenzymatic production of an N-acetylhexosamine 1-phophate analogues library by N-acetylhexosamine 1-kinase (NahK) and describes the respective substrate specificity on this enzyme.
Cai
Li
L
Departments of Chemistry and Biochemistry, The Ohio State University, Columbus, OH 43210, USA.
Guan
Wanyi
W
Kitaoka
Motomitsu
M
Shen
Jie
J
Xia
Chengfeng
C
Chen
Wenlan
W
Wang
Peng George
PG
D000117:Acetylglucosamine / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000737:chemistry / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000138:chemical synthesis / Q000378:metabolism; D001644:Bifidobacterium / Q000201:enzymology*; D007536:Isomerism; D010770:Phosphotransferases / Q000138:chemical synthesis / Q000378:metabolism*; D054852:Small Molecule Libraries / Q000138:chemical synthesis* / Q000737:chemistry / Q000378:metabolism*; D013379:Substrate Specificity
D054852:Small Molecule Libraries; C026146:N-acetylglucosamine-1-phosphate; D000255:Adenosine Triphosphate; D010770:Phosphotransferases; D000117:Acetylglucosamine