[Efficient method of analysis of optical spectra from kinetic studies].
The application of principal components for the analysis of kinetic data obtained by optical spectroscopy is described. The use of singular value decomposition (SVD) for stable and reproducible generation of principal components, details of realization, advantages and drawbacks of the method are discussed. The described method with minor modifications may be used in a wide variety of UV-spectroscopy applications in molecular biology and biophysics. The developed method was applied to study the reaction of platinum anticancer drug, cisplatin, with DNA and methionine. Use of sensitive UV-spectroscopy allowed to study low platinum concentrations, typical for biological systems. It has been shown, that reactions of cisplatin with DNA and L-methionine generally follow the same pathway both at high and low concentrations.
Skvortsov
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D000465:Algorithms; D000818:Animals; D000970:Antineoplastic Agents / Q000378:metabolism / Q000494:pharmacology; D002417:Cattle; D002945:Cisplatin / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D004247:DNA / Q000378:metabolism; D006868:Hydrolysis; D007700:Kinetics; D016018:Least-Squares Analysis; D008715:Methionine / Q000378:metabolism; D013056:Spectrophotometry, Ultraviolet / Q000706:statistics & numerical data*
D000970:Antineoplastic Agents; D004247:DNA; D008715:Methionine; D002945:Cisplatin