Analysis of early hepatic stage schistosomula gene expression by subtractive expressed sequence tags library.
Schistosome parasites require a complex lifecycle requiring two hosts and aquatic phases of development. The schistosomula is a key phase of parasite development within the mammalian host, however relatively little is understood about the molecular processes underlying this stage. In this study 5723 subtractive expressed sequence tags (ESTs) were randomly selected from a 7 day hepatic schistosomula enriched library constructed using suppression subtractive hybridization method. Sequence analysis of these ESTs identified 1762 unique genes (contigs). Among them, 989 contigs were annotated with known genes, 311 contigs were homologous to established genes, 101 contigs were similar to established genes, 72 contigs were weakly similar to established genes and 289 sequences did not match any published sequences. Genes identified related to metabolism, cellular development, immune evasion and host-parasite interactions were identified as enriched in the hepatic schistosomula stage. The future identification of poorly annotated but stage-specific genes may potentially represent new drugs or vaccine targets, applicable for the future controlling of schistosomiasis.
Shanghai Institute of Veterinary Research, Chinese Academy of Agricultural Sciences, Key Laboratory of Animal Parasitology, Ministry of Agriculture of China, PR China.
Xinzhi
X
Wang
Geoffrey N
GN
Gobert
XinGang
X
Feng
Zhiqiang
Z
Fu
Yamei
Y
Jin
Jinbiao
J
Peng
Jiaojiao
J
Lin
D000818:Animals; D020224:Expressed Sequence Tags; D005786:Gene Expression Regulation; D017238:Genes, Helminth / Q000235:genetics*; D015698:Genomic Library; D006790:Host-Parasite Interactions; D008099:Liver / Q000469:parasitology*; D008297:Male; D011817:Rabbits; D015203:Reproducibility of Results; D012549:Schistosoma japonicum / Q000378:metabolism*; D012554:Schistosomiasis japonica / Q000378:metabolism