Protein-lipid interactions: paparazzi hunting for snap-shots.
Photoactivatable groups meeting the criterion of minimal perturbance allow the investigation of interactions in biological samples. Here, we review the application of photoactivatable groups in lipids enabling the study of protein-lipid interactions in (biological) membranes. The chemistry of various photoactivatable groups is summarized and the specificity of the interactions detected is discussed. The recent introduction of 'click chemistry' in photocrosslinking of membrane proteins by photo-activatable lipids opens new possibilities for the analysis of crosslinked products and will help to close the gap between proteomics and lipidomics.
Haberkant
Per
P
Membrane Enzymology, Bijvoet Center and Institute of Biomembranes, Utrecht University, NL-3584 CH Utrecht, The Netherlands. per.haberkant@gmail.com
van Meer
Gerrit
G
D000818:Animals; D003432:Cross-Linking Reagents / Q000737:chemistry; D003978:Diazomethane / Q000031:analogs & derivatives / Q000737:chemistry / Q000528:radiation effects; D006801:Humans; D050356:Lipid Metabolism; D008563:Membrane Lipids / Q000737:chemistry*; D021962:Membrane Microdomains / Q000502:physiology*; D008565:Membrane Proteins / Q000378:metabolism*; D019920:Photoaffinity Labels / Q000737:chemistry; D055668:Photochemical Processes; D025941:Protein Interaction Mapping; D011506:Proteins / Q000378:metabolism*; D040901:Proteomics / Q000379:methods
D003432:Cross-Linking Reagents; D008563:Membrane Lipids; D008565:Membrane Proteins; D019920:Photoaffinity Labels; D011506:Proteins; D003978:Diazomethane