Immunomodulators released during rotavirus infection of polarized caco-2 cells.
Rotavirus preferentially replicates in enterocytes and "danger signals" released by these cells are likely to modulate viral immunity. As a model of these events, we studied selected immunomodulators released during rotavirus infection of polarized Caco-2 cells grown in transwell cultures (TW). At early time points post-infection the virus was detected mainly in the apical side of the TWs, but this tendency was progressively lost concomitantly with disruption of the cell monolayer and cell death. Rotavirus-infected cells released IL-8, PGE(2), small quantities of TGF-beta1, and the constitutive and inducible heat shock proteins HSC70 and HSP70, but not IL-1beta, IL-6, IL-10, IL-12p70, or TNF-alpha. This set of immunomodulators is known to induce a non-inflammatory (non-Th-1) immune response, and may be determining, in part, the relatively low T-cell immune response observed in blood samples after RV infection.
Instituto de Genética Humana, Facultad de Medicina, Pontificia Universidad Javeriana, Bogotá, Colombia.
Luz-Stella
LS
Rodríguez
Alfonso
A
Barreto
Manuel A
MA
Franco
Juana
J
Angel
D018938:Caco-2 Cells; D018929:Cell Culture Techniques / Q000379:methods; D016764:Cell Polarity / Q000276:immunology*; D016207:Cytokines / Q000378:metabolism; D015232:Dinoprostone / Q000378:metabolism; D050883:HSC70 Heat-Shock Proteins / Q000378:metabolism; D018840:HSP70 Heat-Shock Proteins / Q000378:metabolism; D006801:Humans; D007155:Immunologic Factors / Q000378:metabolism*; D016209:Interleukin-8 / Q000378:metabolism; D012401:Rotavirus / Q000502:physiology; D012400:Rotavirus Infections / Q000276:immunology* / Q000378:metabolism* / Q000821:virology; D017201:Virus Shedding
D016207:Cytokines; D050883:HSC70 Heat-Shock Proteins; D018840:HSP70 Heat-Shock Proteins; C494371:HSPA8 protein, human; D007155:Immunologic Factors; D016209:Interleukin-8; D015232:Dinoprostone