SSO1450--a CAS1 protein from Sulfolobus solfataricus P2 with high affinity for RNA and DNA.
Clustered regularly interspaced short palindromic repeats (CRISPR) and their associated protein genes (cas genes) are ubiquitous in archaea and eubacteria. It has been suggested that CRISPR and CAS proteins act as an immune system preventing the invasion of foreign genomic elements at the DNA level. The protein SSO1450 from Sulfolobus solfataricus (Sso) P2 belongs to the CAS1 cluster which is one of the core protein clusters most frequently associated with CRISPR sequences. In this study we show that SSO1450 is a high-affinity nucleic acid binding protein. It binds DNA, RNA and DNA-RNA hybrid apparently sequence non-specific in a multi-site binding mode. Furthermore, SSO1450 promotes the hybridization of complementary nucleic acid strands.
Han
Dong
D
Department of Biochemistry, Universität Bayreuth, Bayreuth, Germany.
Lehmann
Kathleen
K
Krauss
Gerhard
G
D019843:Archaeal Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D001483:Base Sequence; D002874:Chromosome Mapping; D017931:DNA Primers / Q000235:genetics; D019641:DNA, Archaeal / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D019281:Dimerization; D005454:Fluorescence Polarization; D019766:Genes, Archaeal; D055029:Inverted Repeat Sequences; D005810:Multigene Family; D009693:Nucleic Acid Hybridization; D020836:Protein Structure, Quaternary; D019642:RNA, Archaeal / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D016601:RNA-Binding Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D048229:Sulfolobus solfataricus / Q000235:genetics* / Q000378:metabolism*
D019843:Archaeal Proteins; D017931:DNA Primers; D019641:DNA, Archaeal; D004268:DNA-Binding Proteins; D019642:RNA, Archaeal; D016601:RNA-Binding Proteins