Expression of miR-31, miR-125b-5p, and miR-326 in the adipogenic differentiation process of adipose-derived stem cells.
MicroRNAs (miRNAs) are small single-stranded RNAs of 19-22 nucleotides (nt) and are important posttranscriptional regulation of genes. A link between miRNA function and cancer was researched by the miRNAs microarray technology recently. However, during adipogenic differentiation of ADSCs process, this technology was less used to study adipogenic differentiation mechanism of ADSCs. In this study, miRNA microarray technology was used to examine the expression of miRNA that were differences between induced group and noninduced group of ADSC adipogenic differentiation. Real-time quantitative PCR (real-time qPCR) was used to quantify the miRNA expression. The TargetScan 5.0 software was used to find their target genes. Our results showed that the expression of rno-miR-31, rno-miR-125b-5p, and rno-miR-326 were downregulation in the adipogenic differentiation process. By the statistic analysis, this study showed that the expression of rno-miR-31 and rno-miR-326 were significantly deregulation. In addition, the target genes of rno-miR-31 and rno-miR-326 were correlated with the adipogenic differentiation. Our study suggested that the expression of rno-miR-31 and rno-miR-326 were involved in the adipogenic differentiation process.
Tang
Yan-Feng
YF
State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu 610044, People's Republic of China.
Zhang
Yong
Y
Li
Xiao-Yu
XY
Li
Cai
C
Tian
Weidong
W
Liu
Lei
L
D017667:Adipocytes / Q000378:metabolism*; D000818:Animals; D002454:Cell Differentiation / Q000235:genetics*; D019070:Cell Lineage; D002478:Cells, Cultured; D015536:Down-Regulation; D006651:Histocytochemistry / Q000379:methods; D008297:Male; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017207:Rats, Sprague-Dawley; D013234:Stem Cells / Q000166:cytology*
C000605119:MIRN326 microRNA, rat; D035683:MicroRNAs; D012333:RNA, Messenger