An improved Escherichia coli donor strain for diparental mating.
We present a new method for diparental mating with the outstanding advantage that counterselection of the Escherichia coli donor strain is not required. This improved method uses a new donor strain, E. coli ST18, a hemA deletion mutant defective in tetrapyrrole biosynthesis. The hemA mutation can be complemented by addition of 5-aminolevulinic acid. Therefore, counterselection is carried out only using standard media and growth conditions optimal for the recipient strain. Consequently, recipient strains are isolated in a significantly shorter period.
Thoma
Sabrina
S
Schobert
Max
M
D000445:Aldehyde Oxidoreductases / Q000172:deficiency; D000622:Aminolevulinic Acid / Q000378:metabolism; D003227:Conjugation, Genetic; D004251:DNA Transposable Elements; D004926:Escherichia coli / Q000235:genetics*; D018014:Gene Transfer Techniques; D016296:Mutagenesis; D011549:Pseudomonas / Q000235:genetics; D016992:Rhodobacter / Q000235:genetics
D004251:DNA Transposable Elements; D000622:Aminolevulinic Acid; D000445:Aldehyde Oxidoreductases; C067282:glutamyl tRNA reductase