[Influence of antioxidant phenovin and immunomodulator Una de gato on free radical oxidation at parodontitis].
The aim of the research: detection of changes in free radical oxidation at treatment of parodontitis with combination of preparations - antioxidant Phenovin and immunomodulator Una de gato. Reactive compounds of nitrogen, oxygen and lipids in saliva, blood and gingival tissue of patients suffering from moderate form of parodontitis has been studied by means of the electronic paramagnetic resonance (EPR) method and spin-traps (DETC, DMPO, PBN - Sigma). In patients with parodontitis content of free NO in saliva and blood increases, while in gingival tissue - decreases. In saliva, blood and gingival tissue of patients intense EPR signals of superoxidradicals (O2(-)) and lipoperoxides (LOO(-)) has been revealed indicating intensification of processes of lipid peroxidation in oral cavity, as well as in whole organism of patient. Exaggerated synthesis of NO in saliva and blood of patient is determined by high- expression of inducible NO-synthase triggered by oxidative stress, and increased activity of neuronal NO-synthase in saliva as a result of high concentrations of metacholine and P substance intensely secreted at parodontitis. Decreased content of free NO in gingival tissue of patients with parodontitis compared to control is the result of biological degradation of nitric oxide (conversion of NO into peroxinitrite on the background of intense oxidative stress in oral cavity) and nitrosylation of mitochondrial electron transport proteins of gingival tissue (characteristic for parodontitis) with further decrease in content of free oxide, suppression of intensity of mitochondrial respiration, energogenesis, development of ischemia in oral tissue leading to further initiation of destructive processes and progression of parodontitis. Treatment with combination of preparations - Phenovin and Una de gato decreased intensity of oxidative stress in organism of patients and reduced destructive processes of tissues in oral cavity. Relative normalization of oxidative metabolism in organism, restoration of NO-synthase system activity (intensity of EPR signals of spin-trapped nitric oxide and lipoperoxide radicals approach to control data) has been observed. It is concluded that combined treatment of patients suffering from parodontitis using preparations of antioxidant and immunoregulatory activity results in long-term remission of disease.
N N
NN
Gogebashvili
L M
LM
Dzhashi
I V
IV
Datunashvili
N A
NA
Intskirveli
L I
LI
Kiparoidze
D000975:Antioxidants / Q000627:therapeutic use*; D004359:Drug Therapy, Combination; D005609:Free Radicals / Q000378:metabolism; D005881:Gingiva / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D007155:Immunologic Factors / Q000627:therapeutic use*; D010084:Oxidation-Reduction / Q000187:drug effects; D010510:Periodontal Diseases / Q000188:drug therapy* / Q000378:metabolism; D010936:Plant Extracts / Q000627:therapeutic use*; D012463:Saliva / Q000187:drug effects / Q000378:metabolism
D000975:Antioxidants; D005609:Free Radicals; D007155:Immunologic Factors; D010936:Plant Extracts