High-force generation is a conserved property of type IV pilus systems.
The type IV pilus (T4P) system of Neisseria gonorrhoeae is the strongest linear molecular motor reported to date, but it is unclear whether high-force generation is conserved between bacterial species. Using laser tweezers, we found that the average stalling force of single-pilus retraction in Myxococcus xanthus of 149 +/- 14 pN exceeds the force generated by N. gonorrhoeae. Retraction velocities including a bimodal distribution were similar between M. xanthus and N. gonorrhoeae, but force-dependent directional switching was not. Force generation by pilus retraction is energized by the ATPase PilT. Surprisingly, an M. xanthus mutant lacking PilT apparently still retracted T4P, although at a reduced frequency. The retraction velocity was comparable to the high-velocity mode in the wild type at low forces but decreased drastically when the force increased, with an average stalling force of 70 +/- 10 pN. Thus, M. xanthus harbors at least two different retraction motors. Our results demonstrate that the major physical properties are conserved between bacteria that are phylogenetically distant and pursue very different lifestyles.
Clausen
Martin
M
Department for Biology, Westfälische Wilhelms Universität, Münster, Germany.
Jakovljevic
Vladimir
V
Søgaard-Andersen
Lotte
L
Maier
Berenike
B
D000251:Adenosine Triphosphatases / Q000235:genetics / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000378:metabolism; D010861:Fimbriae, Bacterial / Q000502:physiology*; D055786:Gene Knockout Techniques; D020409:Molecular Motor Proteins / Q000378:metabolism*; D009068:Movement; D016941:Myxococcus xanthus / Q000502:physiology*; D009344:Neisseria gonorrhoeae / Q000502:physiology*
D020409:Molecular Motor Proteins; D000255:Adenosine Triphosphate; D000251:Adenosine Triphosphatases