A device to facilitate preparation of high-density neural cell cultures in MEAs.
A device to facilitate high-density seeding of dissociated neural cells on planar multi-electrode arrays (MEAs) is presented in this paper. The device comprises a metal cover with two concentric cylinders-the outer cylinder fits tightly on to the external diameter of a MEA to hold it in place and an inner cylinder holds a central glass tube for introducing a cell suspension over the electrode area of the MEA. An O-ring is placed at the bottom of the inner cylinder and the glass tube to provide a fluid-tight seal between the glass tube and the MEA electrode surface. The volume of cell suspension in the glass tube is varied according to the desired plating density. After plating, the device can be lifted from the MEA without leaving any residue on the contact surface. The device has enabled us to increase and control the plating density of neural cell suspension with low viability, and to prepare successful primary cultures from cryopreserved neurons and glia. The cultures of cryopreserved dissociated cortical neurons that we have grown in this manner remained spontaneously active over months, exhibited stable development and similar network characteristics as reported by other researchers.
Department of Engineering, Faculty of Engineering and Science, Universiti Tunku Abdul Rahman, Malaysia. moksiewying@gmail.com
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Mok
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Lim
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Goh
D000200:Action Potentials / Q000502:physiology; D000818:Animals; D018929:Cell Culture Techniques; D002470:Cell Survival / Q000502:physiology; D002478:Cells, Cultured; D015925:Cryopreservation; D015194:Diffusion Chambers, Culture / Q000295:instrumentation* / Q000379:methods; D004594:Electrophysiology / Q000295:instrumentation* / Q000379:methods; D008839:Microelectrodes; D009415:Nerve Net / Q000166:cytology / Q000502:physiology; D009457:Neuroglia / Q000166:cytology / Q000502:physiology; D009474:Neurons / Q000166:cytology / Q000502:physiology*; D009482:Neurophysiology / Q000295:instrumentation* / Q000379:methods; D051381:Rats