[Screening method of pesticides in meat using cleanup with GPC and mini-column].
A multiresidue screening method using GC/MS and LC/MS was investigated for analysis of 202 pesticides (including metabolites) in meat. Many target pesticides with a wide range of polarity were successfully extracted with ethyl acetate-cyclohexane (1 : 1) from meat samples rich in lipid components. Matrix components such as cholesterol, monoglycerides and fatty acids were effectively eliminated by means of GPC and 2 kinds of mini-columns (PSA and Silica). These procedures enabled analysis in the SCAN mode by GC/MS and LC/MS without any matrix interference. Among the pesticides tested, 17 had low recoveries (<50%), but 185 pesticides showed acceptable recoveries of 50-140% when spiked at 0.1 microg/g into muscle of beef, chicken and pork. The limits of quantitation were 0.01 microg/g, except for linuron, triforine and isoxaflutole (<0.02 microg/g). This proposed method is expected to be useful for screening analysis of residual pesticides in meat.
Hyogo Prefectural Institute of Public Health and Environmental Sciences, Hyogo-ku, Kobe 6520032, Japan.
Tomofumi
T
Matsuoka
Yumi
Y
Akiyama
Takao
T
Mitsuhashi
D000818:Animals; D002417:Cattle; D002645:Chickens; D002850:Chromatography, Gel / Q000295:instrumentation; D002853:Chromatography, Liquid / Q000379:methods*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D013058:Mass Spectrometry / Q000379:methods*; D008460:Meat / Q000032:analysis*; D010573:Pesticide Residues / Q000032:analysis*; D013552:Swine
D010573:Pesticide Residues