Polymorphisms of mtDNA control region in Tunisian and Moroccan populations: an enrichment of forensic mtDNA databases with Northern Africa data.
Current forensic mitochondrial (mt)DNA databases are limited in representative population data of African origin. We investigated HVS-I/HVS-II sequences of 120 Tunisian and Moroccan healthy male donors applying stringent quality criteria to assure high quality of the data and phylogenetic alignment and notation of the sequences. Among 64 Tunisians, 56 different haplotypes were observed and the most common haplotype (16187T 16189C 16223T 16264T 16270T 16278T 16293G 16311C 73G 152C 182T 185T 195C 247A 263G 309.1C 315.1C; haplogroup (hg) L1b) was shared by four individuals. 56 Moroccans could be assigned to 52 different haplotypes where the most common haplotype was of West Eurasian origin with the hg H sequence motif 263G 315.1C and variations in the HVS-II polyC-stretch (309.1C 309.2C) shared by six samples. The majority of the observed haplotypes belong to the west Eurasian phylogeny (50% in Tunisians and 62.5% in Moroccans). Our data are consistent with the current phylogeographic knowledge displaying the occurrence of sub-Saharan haplogroup L sequences, found in 48.4% of Tunisians and 25% of Moroccans as well as the presence of the two re-migrated haplogroups U6 (7.8% and 1.8% in Tunisians and Moroccans, respectively) and M1 (1.6% in Tunisians and 8.9% in Moroccans).
Turchi
Chiara
C
Department of Neuroscience, Section of Legal Medicine, Università Politecnica delle Marche, 60020 Ancona, Italy.
Buscemi
Loredana
L
Giacchino
Erika
E
Onofri
Valerio
V
Fendt
Liane
L
Parson
Walther
W
Tagliabracci
Adriano
A
D017954:Africa South of the Sahara; D000352:Africa, Northern; D022801:Complementarity Determining Regions / Q000235:genetics; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D004272:DNA, Mitochondrial / Q000235:genetics*; D030561:Databases, Nucleic Acid; D004641:Emigration and Immigration; D005006:Ethnicity / Q000235:genetics; D014644:Genetic Variation; D005828:Genetics, Population; D005843:Geography; D017408:Guidelines as Topic; D006239:Haplotypes; D018259:Herpesvirus 1, Human / Q000737:chemistry / Q000235:genetics; D018258:Herpesvirus 2, Human / Q000737:chemistry / Q000235:genetics; D006801:Humans; D019901:Locus Control Region / Q000235:genetics; D008297:Male; D009018:Morocco; D010802:Phylogeny; D011110:Polymorphism, Genetic; D011786:Quality Control; D017422:Sequence Analysis, DNA; D014416:Tunisia
D022801:Complementarity Determining Regions; D004272:DNA, Mitochondrial; D004247:DNA