Rewritable glycochips.
We describe microarraying of carbohydrates for protein screening using either disulfide bridge or Schiff base imine immobilization chemistries on plasmachemical deposited functional nanolayers. The commonly observed issue of nonspecific background binding of proteins is overcome by spotting carbohydrates through a protein-resistant overlayer yielding spatially localized interaction with a reactive functional underlayer.
Department of Chemistry, Science Laboratories, Durham University, Durham DH1 3LE, UK.
L G
LG
Harris
W C E
WC
Schofield
K J
KJ
Doores
B G
BG
Davis
J P S
JP
Badyal
D000814:Aniline Compounds / Q000737:chemistry; D002241:Carbohydrates / Q000737:chemistry*; D003208:Concanavalin A / Q000737:chemistry; D004220:Disulfides / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry; D006018:Glycols / Q000737:chemistry; D008320:Maltose / Q000737:chemistry; D046228:Microarray Analysis / Q000379:methods*; D008856:Microscopy, Fluorescence; D036103:Nanotechnology / Q000379:methods; D019887:Peanut Agglutinin / Q000737:chemistry; D011485:Protein Binding; D017550:Spectroscopy, Fourier Transform Infrared; D013438:Sulfhydryl Compounds / Q000737:chemistry; D013451:Sulfonic Acids / Q000737:chemistry; D014753:Vinyl Compounds / Q000737:chemistry
D000814:Aniline Compounds; D002241:Carbohydrates; D004220:Disulfides; D005456:Fluorescent Dyes; D006018:Glycols; D019887:Peanut Agglutinin; D013438:Sulfhydryl Compounds; D013451:Sulfonic Acids; D014753:Vinyl Compounds; D003208:Concanavalin A; D008320:Maltose; C512132:4-vinylaniline