A novel two-stage cultivation method to optimize carbon concentration and carbon-to-nitrogen ratio for sporulation of biocontrol fungi.
A two-stage solid cultivation method was used to determine the precise requirements of carbon concentration (1-16 g/L) and C: N ratio (0.625: 1 to 80: 1) for the sporulation of six biocontrol fungi. The C concentration and C: N ratio producing the highest conidia yield were 1 g/L and 5: 1 for Paecilomyces lilacinus IPC-P; 2 g/L and 10: 1 for P. lilacinus M-14; 16 g/L and 80: 1 for Metarhizium anisopliae SQZ-1-21; 4 g/L and 5: 1 for M. anisopliae RS-4-1 and Lecanicillium lecanii CA-1-G; and 2 g/L and 10: 1 for Trichoderma viride TV-1. Sporulation was more affected by C: N ratio than by C and N concentration per se. More spores per colony were produced by the two-stage method than by a conventional, single-stage cultivation method. These results should be useful for improving the mass production of these biocontrol agents.
Key Laboratory of Systematic Mycology and Lichenology, Institute of Microbiology, Chinese Academy of Sciences, Chaoyang District, Beijing, 100 010, PR China.
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L
Gao
X Z
XZ
Liu
D000818:Animals; D002244:Carbon / Q000378:metabolism*; D003470:Culture Media / Q000378:metabolism; D046508:Culture Techniques / Q000379:methods*; D005658:Fungi / Q000502:physiology*; D009584:Nitrogen / Q000378:metabolism*; D010572:Pest Control, Biological; D013172:Spores, Fungal / Q000502:physiology*
D003470:Culture Media; D002244:Carbon; D009584:Nitrogen