Thiamine increases beta-glucosidase production in the newly isolated strain of Fomitopsis pinicola.
OBJECTIVE
To isolate a high beta-glucosidase (BGL)-producing strain and to optimize BGL production in the isolated strain.


RESULTS
A high BGL-producing strain was isolated and identified as Fomitopsis pinicola KMJ812 based on its morphology and a comparison of sequence of its internal transcribed spacer rDNA gene. To increase BGL production, F. pinicola was supplemented with various vitamins. Supplementation with thiamine (20 mg l(-1)) improved BGL production in F. pinicola cultures by 3.7-fold to give a specific activity of 114.4 micromol min(-1) mg(-1) protein, one of the highest among BGL-producing micro-organisms. The increased production of BGL in the thiamine-supplemented culture was confirmed by 2D electrophoresis followed by MS/MS sequencing. The BGL purified from F. pinicola culture showed the highest catalytic efficiency ever reported.


CONCLUSIONS
Supplemental thiamine remarkably increased BGL production by a novel BGL-producing strain, F. pinicola KMJ812.


CONCLUSIONS
Our results provide a high BGL-producing strain and the production media for BGL production, and should contribute to better industrial production of glucose via biological processes.
Joo
A-R
AR
Department of Bioscience and Biotechnology, Konkuk University, Seoul, Korea.
Lee
K-M
KM
Sim
W-I
WI
Jeya
M
M
Hong
M-R
MR
Kim
Y-S
YS
Oh
D-K
DK
Lee
J-K
JK
D001483:Base Sequence; D016000:Cluster Analysis; D055453:Coriolaceae / Q000166:cytology / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism*; D003470:Culture Media / Q000737:chemistry; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000235:genetics; D015180:Electrophoresis, Gel, Two-Dimensional; D005656:Fungal Proteins / Q000032:analysis; D006863:Hydrogen-Ion Concentration; D008969:Molecular Sequence Data; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D053719:Tandem Mass Spectrometry; D013696:Temperature; D013831:Thiamine / Q000378:metabolism*; D001617:beta-Glucosidase / Q000096:biosynthesis*
D003470:Culture Media; D004271:DNA, Fungal; D021903:DNA, Ribosomal Spacer; D005656:Fungal Proteins; D001617:beta-Glucosidase; D013831:Thiamine