Digital in-line holography with femtosecond VUV radiation provided by the free-electron laser FLASH.
Femtosecond vacuum ultraviolet (VUV) radiation provided by the free-electron laser FLASH was used for digital in-line holographic microscopy and applied to image particles, diatoms and critical point dried fibroblast cells. To realize the classical in-line Gabor geometry, a 1 microm pinhole was used as spatial filter to generate a divergent light cone with excellent pointing stability. At a fundamental wavelength of 8 nm test objects such as particles and diatoms were imaged at a spatial resolution of 620 nm. In order to demonstrate the applicability to biologically relevant systems, critical point dried rat embryonic fibroblast cells were for the first time imaged with free-electron laser radiation.
Rosenhahn
Axel
A
Angewandte Physikalische Chemie, Universität Heidelberg, Heidelberg, Germany. rosenhahn@uni-heidelberg.de
Staier
Florian
F
Nisius
Thomas
T
Schäfer
David
D
Barth
Ruth
R
Christophis
Christof
C
Stadler
Lorenz-M
LM
Streit-Nierobisch
Simone
S
Gutt
Christian
C
Mancuso
Adrian
A
Schropp
Andreas
A
Gulden
Johannes
J
Reime
Bernd
B
Feldhaus
Josef
J
Weckert
Edgar
E
Pfau
Bastian
B
Günther
Christian M
CM
Könnecke
René
R
Eisebitt
Stefan
S
Martins
Michael
M
Faatz
Bart
B
Guerassimova
Natalia
N
Honkavaara
Katja
K
Treusch
Rolf
R
Saldin
Evgueni
E
Schreiber
Siegfried
S
Schneidmiller
Evgeny A
EA
Yurkov
Mikhail V
MV
Vartanyants
Ivan
I
Grübel
Gerhard
G
Grunze
Michael
M
Wilhein
Thomas
T
D000818:Animals; D017377:Diatoms / Q000166:cytology; D004583:Electrons; D006696:Holography / Q000379:methods*; D007834:Lasers; D051381:Rats; D012822:Silicon Dioxide; D013997:Time Factors; D014466:Ultraviolet Rays; D014618:Vacuum
D012822:Silicon Dioxide