Effect of systemic tetracycline on the degradation of tetracycline-impregnated bilayered collagen membranes: an animal study.
BACKGROUND
Premature collagen membrane degradation may compromise the outcome of osseous regenerative procedures. Tetracyclines (TTCs) inhibit the catalytic activities of human metalloproteinases. Preprocedural immersion of collagen membranes in TTC and systemic administration of TTC may be possible alternatives to reduce the biodegradation of native collagen membranes.


OBJECTIVE
To evaluate the in vivo degradation of collagen membranes treated by combined TTC immersion and systemic administration.


METHODS
Seventy-eight bilayered porcine collagen membrane disks were divided into three groups and were immersed in 0, 50, or 100 mg/mL TTC solution. Three disks, one of each of the three groups, were implanted on the calvaria of each of 26 Wistar rats. Thirteen (study group) were administered with systemic TTC (10 mg/kg), while the remaining 13 received saline injections (control group). Calvarial tissues were retrieved after 3 weeks, and histological sections were analyzed by image analysis software.


RESULTS
Percentage of remaining collagen area within nonimpregnated membranes was 52.26 ± 20.67% in the study group, and 32.74 ± 13.81% in the control group. Immersion of membranes in 100 mg/mL TTC increased the amount of residual collagen to 63.46 ± 18.19% and 42.82 ± 12.99% (study and control groups, respectively). Immersion in 50 mg/mL TTC yielded maximal residual collagen values: 80.75 ± 14.86% and 59.15 ± 8.01% (study and control groups, respectively). Differences between the TTC concentrations, and between the control and the study groups were statistically significant.


CONCLUSIONS
Immersion of collagen membranes in TTC solution prior to their implantation and systemic administration of TTC significantly decreased the membranes' degradation.
Department of Periodontology, The Maurice and Gabriela Goldschleger School of Dental Medicine, Tel Aviv University, Tel Aviv, Israel. moseso@post.tau.ac.il
Ofer
O
Moses
Tami
T
Frenkel
Haim
H
Tal
Miron
M
Weinreb
Michael M
MM
Bornstein
Carlos E
CE
Nemcovsky
D020341:Absorbable Implants; D000818:Animals; D000900:Anti-Bacterial Agents / Q000008:administration & dosage*; D001711:Biotransformation / Q000187:drug effects*; D003094:Collagen / Q000187:drug effects*; D005260:Female; D016557:Guided Tissue Regeneration, Periodontal; D008567:Membranes, Artificial; D051381:Rats; D017208:Rats, Wistar; D013752:Tetracycline / Q000008:administration & dosage*
D000900:Anti-Bacterial Agents; D008567:Membranes, Artificial; D003094:Collagen; D013752:Tetracycline