Hepatoprotective studies on Sida acuta Burm. f.
BACKGROUND
Sida acuta Burm. f. (Malvaceae) is used in Indian traditional medicine to treat liver disorders and is useful in treating nervous and urinary diseases and also disorders of the blood and bile.


OBJECTIVE
Evaluation of the hepatoprotective properties of the methanolic extract of the root of Sida acuta (SA) and the phytochemical analysis of SA.


METHODS
The model of paracetamol-induced hepatotoxicity in Wistar rats, liver histopathological observations, hexobarbitone-induced narcosis and in vitro anti-lipid peroxidation studies were employed to assess the hepatoprotective efficacy of SA. Phytochemical assay of SA was conducted following standard protocols.


RESULTS
Significant hepatoprotective effects were obtained against liver damage induced by paracetamol overdose as evident from decreased serum levels of glutamate pyruvate transaminase, glutamate oxaloacetate transaminase, alkaline phosphatase and bilirubin in the SA treated groups (50, 100, 200mg/kg) compared to the intoxicated controls. The hepatoprotective effect was further verified by histopathology of the liver. Pretreatment with Sida acuta extract significantly shortened the duration of hexobarbitone-induced narcosis in mice indicating its hepatoprotective potential. Phytochemical studies confirmed the presence of the phenolic compound, ferulic acid in the root of Sida acuta, which accounts for the significant hepatoprotective effects observed in the present study.


CONCLUSIONS
The present study thus provides a scientific rationale for the traditional use of this plant in the management of liver disorders.
Department of Biotechnology, Mar Athanasius College, Kothamangalam 686 666, India.
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Sreedevi
P G
PG
Latha
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Ancy
S R
SR
Suja
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S
Shyamal
V J
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Shine
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Sini
G I
GI
Anuja
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Rajasekharan
D000082:Acetaminophen; D000469:Alkaline Phosphatase / Q000097:blood; D000818:Animals; D001663:Bilirubin / Q000097:blood; D056486:Chemical and Drug Induced Liver Injury / Q000097:blood / Q000188:drug therapy*; D003373:Coumaric Acids / Q000032:analysis / Q000494:pharmacology*; D006591:Hexobarbital; D008099:Liver / Q000187:drug effects* / Q000473:pathology; D008297:Male; D019660:Malvaceae / Q000737:chemistry*; D051379:Mice; D008517:Phytotherapy; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D018517:Plant Roots; D020011:Protective Agents / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D053608:Stupor / Q000097:blood / Q000188:drug therapy; D000637:Transaminases / Q000097:blood
D003373:Coumaric Acids; D010936:Plant Extracts; D020011:Protective Agents; D000082:Acetaminophen; D006591:Hexobarbital; C004999:ferulic acid; D000637:Transaminases; D000469:Alkaline Phosphatase; D001663:Bilirubin