[Comparative research on the effects of different nutrient concentrations on the photopigment content and photosynthesis of two bloom-forming species isolated from the Changjiang River estuary].
The contents of cellular chlorophyll a (Chl-a), chlorophyll c (Chl-c), total coloured carotenoids (TCC) and the photosynthesis of Skeletonema costatum and Prorocentrum donghaiense under different nutrient conditions were studied. The results showed that both species in the low nutrient concentration conditions had lower cellular Chl-a, Chl-c and TCC content than those in the high nutrient concentration conditions. When the initial N/P ratio was 16/1 while the concentrations were different, the two species had different photosynthetic rate responses. For S. costatum, the photosynthetic rate normalized by cell in the low nutrient concentration group (64 micromol/L N and 4 micromol/L P) was significantly lower than that in the high concentration group (256 micromol/L N and 16 micromol/L P) from the fifth day, and at the end of the experiment (on the seventh day), the photosynthetic rate was 0.031 x 10(-4) micromol x (cell x h)(-1) and 0.13 x 10(-4) micromol x (cell x h)(-1) respectively, while the photosynthetic rate normalized by Chl-a was 12.92 micromol x (microg x h)(-1) and 13.03 micromol x (microg x h)(-1) for the two groups respectively, and there was no significant difference between them; however, for P. donghaiense, the photosynthetic rates normalized by both cell and Chl-a in the low concentration conditions (64 micromol/L N and 4 micromol/L P) were significantly higher than those in the high concentration conditions (256 micromol/L N and 16 micromol/L P). For the two species, when P was sufficient, the low N concentration group (64 micromol/L N and 36 micromol/L P) and when N was sufficient, the low P concentration group (883 micromol/L N and 4 micromol/L P) had higher photosynthetic rates normalized by both cell and Chl-a than the high N concentration group (256 micromol/L N and 36 micromol/L P) and the high P concentration group (883 micromol/L N and 16 micromol/L P) respectively. There was a significant positive relationship between the photosynthetic rate and the intracellular P for both species, and P. donghaiense had higher cellular photopigment contents and photosynthetic rates than S. costatum under the same nutrient conditions. It could be inferred from their photosynthetic characteristics that P. donghaiense would survive better in low nutrient conditions compared with S. costatum.
Key Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China. zhaoyanfang@qdio.ac.cn
Yan-Fang
YF
Zhao
Zhi-Ming
ZM
Yu
Xiu-Xian
XX
Song
Xi-Hua
XH
Cao
D002338:Carotenoids / Q000378:metabolism; D002734:Chlorophyll / Q000378:metabolism*; D000077194:Chlorophyll A; D017377:Diatoms / Q000254:growth & development / Q000378:metabolism*; D004141:Dinoflagellida / Q000254:growth & development / Q000378:metabolism*; D005618:Fresh Water / Q000032:analysis; D009584:Nitrogen / Q000494:pharmacology*; D010758:Phosphorus / Q000494:pharmacology*; D010788:Photosynthesis; D045483:Rivers; D012623:Seawater / Q000032:analysis
D002734:Chlorophyll; D010758:Phosphorus; D002338:Carotenoids; D009584:Nitrogen; D000077194:Chlorophyll A