Pathogenicity of different strains of Histophilus somni in the experimental induction of ovine epididymitis.
The purpose of this study was to determine any differences in pathogenicity when sheep are experimentally infected with different Histophilus somni isolates: a) 2336 bovine origin strain; b) an isolate from ram orchitis and epididymitis; c) an isolate from the brain of a sheep with neurological signs; d) an isolate from the vagina of a clinically healthy ewe. A total of 20 rams divided in groups of 5 animals each were inoculated in the epididymis with 1 x 10(7) CFU/mL of H. somni; a negative control group of 5 rams was used. All groups inoculated with H. somni showed some epididymitis, but the most pathology was caused by the epididymitis isolate, followed by the vaginal isolate. It was demonstrated that there is a difference in experimental infection capacity among isolates from different origins, as epididymitis occurred and the bacteria was recovered only from groups inoculated with isolates originating from epididymitis and vaginal exudate.
CENID Microbiología, Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias, Carretera Federal México-Toluca Km. 15.5, Cuajimalpa DF. CP 05110, México. efredia@yahoo.com
Efrén
E
Díaz-Aparicio
Víctor R
VR
Tenorio-Gutiérrez
Beatriz
B
Arellano-Reynoso
Idalia
I
Enríquez-Verdugo
Francisco
F
Aguilar-Romero
D000818:Animals; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004823:Epididymitis / Q000382:microbiology / Q000662:veterinary*; D006651:Histocytochemistry / Q000662:veterinary; D008297:Male; D015661:Pasteurellaceae / Q000235:genetics / Q000472:pathogenicity*; D016871:Pasteurellaceae Infections / Q000382:microbiology / Q000662:veterinary*; D016133:Polymerase Chain Reaction / Q000662:veterinary; D011897:Random Allocation; D012756:Sheep; D012757:Sheep Diseases / Q000382:microbiology*; D014774:Virulence
D004269:DNA, Bacterial