Evolution of complex RNA polymerases: the complete archaeal RNA polymerase structure.
The archaeal RNA polymerase (RNAP) shares structural similarities with eukaryotic RNAP II but requires a reduced subset of general transcription factors for promoter-dependent initiation. To deepen our knowledge of cellular transcription, we have determined the structure of the 13-subunit DNA-directed RNAP from Sulfolobus shibatae at 3.35 Å resolution. The structure contains the full complement of subunits, including RpoG/Rpb8 and the equivalent of the clamp-head and jaw domains of the eukaryotic Rpb1. Furthermore, we have identified subunit Rpo13, an RNAP component in the order Sulfolobales, which contains a helix-turn-helix motif that interacts with the RpoH/Rpb5 and RpoA'/Rpb1 subunits. Its location and topology suggest a role in the formation of the transcription bubble.
Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut, USA.
Yakov
Y
Korkhin
Ulug M
UM
Unligil
Otis
O
Littlefield
Pamlea J
PJ
Nelson
David I
DI
Stuart
Paul B
PB
Sigler
Stephen D
SD
Bell
Nicola G A
NG
Abrescia
D000595:Amino Acid Sequence; D019843:Archaeal Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D018360:Crystallography, X-Ray; D012321:DNA-Directed RNA Polymerases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D019143:Evolution, Molecular; D008958:Models, Molecular; D008969:Molecular Sequence Data; D021122:Protein Subunits / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D019642:RNA, Archaeal / Q000235:genetics / Q000378:metabolism*; D016944:Sulfolobus / Q000737:chemistry / Q000201:enzymology* / Q000235:genetics; D014158:Transcription, Genetic
D019843:Archaeal Proteins; D021122:Protein Subunits; D019642:RNA, Archaeal; D012321:DNA-Directed RNA Polymerases