[Effect of the gardenia extracts-T9 on viral replication and IFN-gamma mRNA in Herpes simplex virus type-1 infected mice brains].
RT-PCR was used to detect expression level of VP16 mRNA and IFN-gamma mRNA in Herpes simplex virus type-1 infected mice brains at 4th day, 7th day, 10th day, 14th day, 21st day post infection and investigate the effects of the Gardenia extracts-T9 on viral replication and host immunity. The results showed that expression of VP16 mRNA in Gardenia extracts-T9 high dose and low dose group were both lower than that in virus control group at same time point. Relative VP16 mRNA expression in low dose group decreased at 21st day and relative VP16 mRNA expression in high dose group decreased continuously. Relative expression of IFN-gamma mRNA in high dose and low dose groups were both higher than that in virus control group at all time point except the 4th day. IFN-gamma mRNA in low dose group increased from the 4th day till the 14th day, and after the 14th day, the expression decreased slightly. Relative IFN-gamma mRNA in high dose group maintained increasing from 4th day till 21st day. Base on these results, we conclude that Gardenia extracts-T9 might exert the inhibition effect of viral replication by upregulating expression of IFN-gamma mRNA.
Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China. yujingshi@hotmail.com
Yu-jing
YJ
Shi
Yang
Y
Huang
Jing
J
Jiang
Shan-shan
SS
Guo
Dan
D
Su
Ye
Y
Zhao
Ying-jie
YJ
Gao
Xiao-lan
XL
Cui
D000818:Animals; D001921:Brain / Q000378:metabolism* / Q000821:virology*; D005260:Female; D032062:Gardenia / Q000737:chemistry*; D015967:Gene Expression Regulation, Viral / Q000187:drug effects / Q000235:genetics; D018259:Herpesvirus 1, Human / Q000187:drug effects* / Q000254:growth & development* / Q000472:pathogenicity; D007371:Interferon-gamma / Q000235:genetics*; D051379:Mice; D008807:Mice, Inbred BALB C; D010936:Plant Extracts / Q000008:administration & dosage / Q000494:pharmacology*; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000235:genetics; D014779:Virus Replication / Q000187:drug effects*
D010936:Plant Extracts; D012333:RNA, Messenger; D007371:Interferon-gamma