Chitiniphilus shinanonensis gen. nov., sp. nov., a novel chitin-degrading bacterium belonging to Betaproteobacteria.
A bacterial strain capable of degrading chitin, strain SAY3T, was isolated from moat water of Ueda Castle in Nagano Prefecture, Japan. The strain was gram-negative, curved rod-shaped, facultatively anaerobic, and motile with a single polar flagellum. It grew well with chitin as a sole carbon source. The cellular fatty acids profiles showed the presence of C16:1 omega7c and C16:0 as the major components. The G+C content of DNA was 67.6 mol% and Q-8 was the major respiratory quinone. A 16S rRNA gene sequence-based phylogenetic analysis showed the strain belonged to the family Neisseriaceae but was distantly related (94% identity) to any previously known species. Since the strain was clearly distinct from closely related genera in phenotypic and chemotaxonomic characteristics, it should be classified under a new genus and a new species. We propose the name Chitiniphilus shinanonensis gen. nov., sp. nov. The type strain is SAY3T (=NBRC 104970T=NICMB 14509T).
Sato
Kazuaki
K
Division of Applied Biology, Faculty of Textile Science and Technology, Shinshu University, Nagano, Japan.
Kato
Yuichi
Y
Taguchi
Goro
G
Nogawa
Masahiro
M
Yokota
Akira
A
Shimosaka
Makoto
M
D002633:Chemotaxis; D002686:Chitin / Q000378:metabolism*; D046529:Microscopy, Electron, Transmission; D008969:Molecular Sequence Data; D009346:Neisseriaceae / Q000145:classification* / Q000201:enzymology / Q000235:genetics / Q000378:metabolism* / Q000648:ultrastructure; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D014871:Water Microbiology
D012336:RNA, Ribosomal, 16S; D002686:Chitin