A field study to estimate the prevalence of bovine African Trypanosomosis in Butaleja District, Uganda.
Prevalence of bovine trypanosomosis was determined from a total of 203 blood samples collected from Butaleja district, eastern Uganda. All samples were examined by microhematocrit centrifuge test (MHC), PCR and ELISA. ELISA was performed in accordance with the OIE standard procedures using Trypanosoma brucei gambiense procyclic form crude antigens. PCR were utilized to identify the species and the subspecies of trypanosome. The overall prevalence of bovine African trypanosomosis was 8.9% by MHC, and 45.3% by the ELISA. Since substantial number (12 out of 18) of MHC positive samples were negative in the PCR tests, we could not conclude the most epidemic trypanosome species in the studied area. Nevertheless, the PCR results suggests that the most prevalent trypanosome was T. b. brucei (31/203), followed by T. congolense (6/203). In addition, only a few (3/203) mixed infections of T. b. brucei and T. congolense was detected by the PCR. Results obtained from this study indicates that bovine trypanosomosis is endemic in Butaleja district, Uganda.
National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Japan.
Zhang
Z
Jing
Joseph W
JW
Magona
Tatsuya
T
Sakurai
Oriel M M
OM
Thekisoe
Charles P
CP
Otim
Chihiro
C
Sugimoto
Noboru
N
Inoue
D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood; D002417:Cattle; D016054:DNA, Protozoan / Q000737:chemistry / Q000235:genetics; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D006400:Hematocrit / Q000662:veterinary; D016133:Polymerase Chain Reaction / Q000662:veterinary; D016036:Seroepidemiologic Studies; D014345:Trypanosoma / Q000235:genetics / Q000302:isolation & purification*; D014354:Trypanosomiasis, Bovine / Q000453:epidemiology* / Q000469:parasitology; D014454:Uganda / Q000453:epidemiology
D000913:Antibodies, Protozoan; D016054:DNA, Protozoan