Myrosinases, TGG1 and TGG2, redundantly function in ABA and MeJA signaling in Arabidopsis guard cells.
Thioglucoside glucohydrolase (myrosinase), TGG1, is a strikingly abundant protein in Arabidopsis guard cells. We investigated responses of tgg1-3, tgg2-1 and tgg1-3 tgg2-1 mutants to abscisic acid (ABA) and methyl jasmonate (MeJA) to clarify whether two myrosinases, TGG1 and TGG2, function during stomatal closure. ABA, MeJA and H(2)O(2) induced stomatal closure in wild type, tgg1-3 and tgg2-1, but failed to induce stomatal closure in tgg1-3 tgg2-1. All mutants and wild type showed Ca(2+)-induced stomatal closure and ABA-induced reactive oxygen species (ROS)production. A model is discussed in which two myrosinases redundantly function downstream of ROS production and upstream of cytosolic Ca(2+) elevation in ABA and MeJA signaling in guard cells.
Graduate School of Natural Science and Technology, Okayama University, 3-1-1 Tsushima-naka, Okayama, Japan.
Mohammad Mahbub
MM
Islam
Chiharu
C
Tani
Megumi
M
Watanabe-Sugimoto
Misugi
M
Uraji
Md Sarwar
MS
Jahan
Choji
C
Masuda
Yoshimasa
Y
Nakamura
Izumi C
IC
Mori
Yoshiyuki
Y
Murata
D000040:Abscisic Acid / Q000378:metabolism*; D000085:Acetates / Q000378:metabolism*; D017360:Arabidopsis / Q000201:enzymology / Q000235:genetics*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D002118:Calcium / Q000378:metabolism; D003517:Cyclopentanes / Q000378:metabolism*; D006026:Glycoside Hydrolases / Q000235:genetics / Q000378:metabolism*; D006861:Hydrogen Peroxide / Q000378:metabolism; D009154:Mutation; D054883:Oxylipins / Q000378:metabolism*; D010937:Plant Growth Regulators / Q000378:metabolism; D054046:Plant Stomata / Q000502:physiology; D015398:Signal Transduction
D000085:Acetates; D029681:Arabidopsis Proteins; D003517:Cyclopentanes; D054883:Oxylipins; D010937:Plant Growth Regulators; D000040:Abscisic Acid; C072239:methyl jasmonate; D006861:Hydrogen Peroxide; D006026:Glycoside Hydrolases; C511862:TGG1 protein, Arabidopsis; C511863:TGG2 protein, Arabidopsis; D002118:Calcium