Cloning and expression of cDNA for interleukin 4 from the MSKR inbred strain of Mus musculus molossinus.
The 5'-upsteam region of the genomic gene and cDNA encoding interleukin 4 (IL-4) has been isolated and sequenced from the inbred mouse strain MSKR derived from a Japanese wild mouse using polymerase chain reaction (PCR) methodology. The cDNA sequence of IL-4 of MSKR was found to contain several nucleotide alterations, which result in amino acid substitutions, in comparison with that of inbred mouse BALB/c. In MSKR, IL-4 was expressed at high levels in thymus and spleen as revealed by reverse transcriptase PCR method and Northern hybridization. The tissue-specific expression profile was quite similar to that of laboratory mice.
Department of Molecular Immunology, The Jikei University School of Medicine, 3-25-8 Nishi-shinbashi, Minato-ku, Tokyo 105-8461, Japan. ikeshima@jikei.ac.jp
Hiroko
H
Ikeshima-Kataoka
Adumi
A
Wada
Kenji
K
Ishiwata
Naohiro
N
Watanabe
Saburo
S
Saito
D000818:Animals; D017849:Animals, Inbred Strains; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000378:metabolism*; D005786:Gene Expression Regulation; D015847:Interleukin-4 / Q000235:genetics* / Q000502:physiology; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012689:Sequence Homology, Nucleic Acid; D013950:Thymus Gland / Q000166:cytology; D014018:Tissue Distribution
D018076:DNA, Complementary; D015847:Interleukin-4