Isolation of dihydrocurcuminoids from cell clumps and their distribution in various parts of turmeric (Curcuma longa).
In addition to well-known curcuminoids, three colored metabolites were isolated from cultured cell clumps that had been induced from buds on turmeric rhizomes. The isolated compounds were identified as dihydro derivatives of curcuminoids, dihydrocurcumin (dihydroCurc), dihydrodesmethoxycurcumin-a (dihydroDMC-a), and dihydrobisdesmethoxycurcumin (dihydroBDMC). The cell clumps did not contain dihydroDMC-b, an isomer of dihydroDMC-a. A comparison of the distribution profiles of curcuminoids and dihydrocurcuminoids in the cell clumps with those in the rhizomes, leaves, and roots revealed the following differences: Unlike rhizomes, the cell clumps, leaves, and roots contained dihydrocurcuminoids as the major colored constituents. Whereas dimethoxy compounds, curcumin and dihydrocurcumin, respectively, were most abundant in the rhizomes and leaves, one of the monomethoxy derivatives, dihydroDMC-a, was found most abundantly in the cell clumps and roots. While both dihydroDMC-a and b were detected in the rhizomes, dihydroDMC-b was not detectable in the cell clumps, leaves, or roots. The occurrence of only one of the two possible isomers of dihydroDMC suggests biosynthetic formation of dihydrocurcuminoids in turmeric.
Kita
Tomoko
T
Somatech Center, House Foods Corporation, Yotsukaido, Chiba, Japan. t-kita@housefoods.co.jp
Imai
Shinsuke
S
Sawada
Hiroshi
H
Seto
Haruo
H
D002478:Cells, Cultured; D030024:Curcuma / Q000166:cytology* / Q000378:metabolism*; D003474:Curcumin / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D018514:Plant Structures / Q000166:cytology* / Q000378:metabolism*
D003474:Curcumin