Rapid modification of retroviruses using lipid conjugates.
Methods are needed to manipulate natural nanoparticles. Viruses are particularly interesting because they can act as therapeutic cellular delivery agents. Here we examine a new method for rapidly modifying retroviruses that uses lipid conjugates composed of a lipid anchor (1,2-distearoyl-sn-glycero-3-phosphoethanolamine), a polyethylene glycol chain, and biotin. The conjugates rapidly and stably modified retroviruses and enabled them to bind streptavidin. The implication of this work for modifying viruses for gene therapy and vaccination protocols is discussed.
The Wallace H Coulter Department of Biomedical Engineering at Georgia Tech and Emory University, Atlanta, GA 30332-0535, USA.
Nimisha G
NG
Mukherjee
L Andrew
LA
Lyon
Joseph M
JM
Le Doux
D002460:Cell Line; D006801:Humans; D010743:Phospholipids / Q000378:metabolism*; D012190:Retroviridae / Q000737:chemistry*; D019809:Streptavidin / Q000378:metabolism; D053585:Virus Attachment; D018406:Virus Physiological Phenomena
D010743:Phospholipids; D019809:Streptavidin