Living-cell microarrays.
Living cells are remarkably complex. To unravel this complexity, living-cell assays have been developed that allow delivery of experimental stimuli and measurement of the resulting cellular responses. High-throughput adaptations of these assays, known as living-cell microarrays, which are based on microtiter plates, high-density spotting, microfabrication, and microfluidics technologies, are being developed for two general applications: (a) to screen large-scale chemical and genomic libraries and (b) to systematically investigate the local cellular microenvironment. These emerging experimental platforms offer exciting opportunities to rapidly identify genetic determinants of disease, to discover modulators of cellular function, and to probe the complex and dynamic relationships between cells and their local environment.
Center for Engineering in Medicine and Surgical Services, Massachusetts General Hospital, Harvard Medical School, Massachusetts 02139, USA. ireis@sbi.org
Martin L
ML
Yarmush
Kevin R
KR
King
D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods; D022082:Cellular Structures; D023281:Genomics; D049452:Green Fluorescent Proteins / Q000737:chemistry; D006801:Humans; D008280:Magnetics; D046210:Microfluidic Analytical Techniques; D044085:Microfluidics; D055616:Microtechnology; D020411:Oligonucleotide Array Sequence Analysis; D034622:RNA Interference; D013997:Time Factors; D014162:Transfection
D049452:Green Fluorescent Proteins