Resistance to macrolides by ribosomal mutation in clinical isolates of Turicella otitidis.
The genetic basis of erythromycin resistance in Turicella otitidis, a coryneform bacteria associated with otitis, was studied in five macrolide-resistant clinical isolates. Macrolide resistance genes were searched for by polymerase chain reaction (PCR). Genes for domain V of 23S rRNA (rrl) as well as rplD (L4 protein) and rplV (L22 protein) genes were characterised, amplified by PCR from total genomic DNA and sequenced. In the resistant isolates, cross-resistance to macrolides and clindamycin was associated with mutations at positions 2058 and/or 2059 (Escherichia coli numbering). Three isolates displayed A2058 mutations, one isolate had an A2059G mutation whereas another one contained mutations at positions 2058 and 2059. Southern blot experiments revealed that T. otitidis had three copies of the rrl gene. In conclusion, resistance to macrolides in T. otitidis is due, at least in part, to mutations in the rrl gene.
Boumghar-Bourtchai
Leyla
L
EA 2128 Interactions hôte et microorganismes des épithéliums, University of Caen, Caen, France.
Chardon
Hubert
H
Malbruny
Brigitte
B
Mezghani
Senda
S
Leclercq
Roland
R
Dhalluin
Anne
A
D019943:Amino Acid Substitution; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001426:Bacterial Proteins / Q000235:genetics; D039941:Bifidobacteriales Infections / Q000382:microbiology*; D001644:Bifidobacterium / Q000187:drug effects* / Q000235:genetics* / Q000302:isolation & purification; D002981:Clindamycin / Q000494:pharmacology; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics*; D018628:Gene Dosage; D006801:Humans; D018942:Macrolides / Q000494:pharmacology*; D012338:RNA, Ribosomal, 23S / Q000235:genetics*; D012269:Ribosomal Proteins / Q000235:genetics; D017422:Sequence Analysis, DNA
D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D004269:DNA, Bacterial; C040045:L20 ribosomal protein, bacteria; D018942:Macrolides; D012338:RNA, Ribosomal, 23S; D012269:Ribosomal Proteins; C056368:ribosomal protein L4; D002981:Clindamycin