Limited antioxidant effect after consumption of a single dose of tomato sauce by young males, despite a rise in plasma lycopene.
This study investigated the effect of a single dose of tomato sauce on healthy male volunteers in a randomized crossover study. Healthy male subjects (n = 10) were enrolled. Placebo (rice and olive oil) or tomato (tomato sauce, rice and olive oil) meals were provided to the volunteers. Blood and urine samples were taken before consumption of meal (0 h) and 2, 4, 6, 24 and 48 h after meal. Consumption of tomato sauce increased plasma lycopene level by 5-22%, with a maximum level at 24 h (p<0.01) after the meal. Levels of plasma F(2)-isoprostanes, hydroxyeicosatetraenoic acid products, allantoin and urinary 8-hydroxy-2'-deoxyguanosine did not change after either meal, but urinary F(2)-isoprostanes (p<0.05) significantly decreased at 48 h compared to 0 h after the tomato sauce meal. This study showed that a single dose of tomato sauce meal had only a limited antioxidant effect in vivo.
Lee
Chung-Yung Jetty
CY
Department of Biochemistry, Yong Loo Lin School of Medicine, National University of Singapore, Singapore.
Isaac
Helen B
HB
Huang
Shan Hong
SH
Long
Lee Hua
LH
Wang
Huansong
H
Gruber
Jan
J
Ong
Choon Nam
CN
Kelly
Ronan P
RP
Halliwell
Barry
B
D000328:Adult; D000481:Allantoin / Q000097:blood; D000975:Antioxidants / Q000008:administration & dosage*; D015415:Biomarkers / Q000032:analysis*; D002338:Carotenoids / Q000097:blood*; D002851:Chromatography, High Pressure Liquid; D018592:Cross-Over Studies; D003839:Deoxyadenosines / Q000652:urine; D004032:Diet; D028441:F2-Isoprostanes / Q000097:blood / Q000652:urine; D006801:Humans; D006893:Hydroxyeicosatetraenoic Acids / Q000097:blood; D000077276:Lycopene; D018551:Solanum lycopersicum; D008297:Male
C436623:8-hydroxy-2'-deoxyadenosine; D000975:Antioxidants; D015415:Biomarkers; D003839:Deoxyadenosines; D028441:F2-Isoprostanes; D006893:Hydroxyeicosatetraenoic Acids; D000481:Allantoin; D002338:Carotenoids; D000077276:Lycopene