Metals and linear alkylbenzene sulphonate as inhibitors of the algae Pseudokirchneriella subcapitata acid phosphatase activity.
Sewage sludge applied to soils as a fertilizer often contains metals and linear alkylbenzene sulphonate (LAS) as contaminants. These pollutants can be transported to the aquatic environment where they can alter the phosphatase activity in living organisms. The acid phosphatase of algae plays important roles in metabolism such as decomposing organic phosphate into free phosphate and autophagic digestive processes. The order of in vitro inhibition of Pseudokirchneriella subcapitata acid phosphatase at the highest concentration tested was LAS > Hg2+ = Al3+ > Se4+ = Pb2+ > Cd2+. A non-competitive inhibition mechanism was obtained for Hg2+ (Ki = 0.040 mM) and a competitive inhibition for LAS (Ki = 0.007 mM). In vivo studies with treated algae cultures showed that the inhibition of specific activity was observed in algae exposed during 7 days, in contrast to short term (24 h) treatments with both these chemicals. Our results suggest that the inhibition parameters in vitro did not markedly differ between the two chemicals. On the other hand, in vivo evaluations showed strong differences between both pollutants regarding the concentration values and the degree of response.
Embrapa Meio Ambiente, CP 69, Jaguariúna, São Paulo 13820-000, Brazil. jonsson@cnpma.embrapa.br
Claudio Martín
CM
Jonsson
Lourival Costa
LC
Paraiba
Hiroshi
H
Aoyama
D000135:Acid Phosphatase / Q000037:antagonists & inhibitors* / Q000302:isolation & purification; D020418:Algal Proteins / Q000037:antagonists & inhibitors* / Q000302:isolation & purification; D017738:Alkanesulfonic Acids / Q000737:chemistry* / Q000494:pharmacology; D056890:Eukaryota / Q000187:drug effects / Q000201:enzymology*; D020128:Inhibitory Concentration 50; D019216:Metals, Heavy / Q000737:chemistry* / Q000494:pharmacology; D014874:Water Pollutants, Chemical / Q000737:chemistry* / Q000494:pharmacology
D020418:Algal Proteins; D017738:Alkanesulfonic Acids; D019216:Metals, Heavy; D014874:Water Pollutants, Chemical; C030641:alkylbenzyl sulfonic acid; D000135:Acid Phosphatase