Oral vaccination against mycoplasmal pneumonia of swine using a live Erysipelothrix rhusiopathiae vaccine strain as a vector.
Erysipelothrix rhusiopathiae Koganei 65-0.15 strain, the live swine erysipelas vaccine for subcutaneous injection, has been shown to colonize the tonsils of pigs after oral inoculation. We thus evaluated the possible use of the strain as a vector for oral vaccination against mycoplasmal pneumonia of swine. Recombinant E. rhusiopathiae strains expressing the C-terminal domain of the P97 adhesin of Mycoplasma hyopneumoniae were constructed and examined for vaccine efficacy in mice and pigs. Mice subcutaneously inoculated with the recombinant strains were protected from challenge exposure to a virulent E. rhusiopathiae. Administration of milk replacer containing recombinant E. rhusiopathiae expressing the M. hyopneumoniae protein protected pigs from death after exposure to E. rhusiopathiae and significantly reduced the severity of pneumonic lung lesions caused by infection with M. hyopneumoniae.
National Institute of Animal Health, 3-1-5 Kannondai, Tsukuba, Ibaraki 305-0856, Japan.
Yohsuke
Y
Ogawa
Eiji
E
Oishi
Yoshihiro
Y
Muneta
Akiyuki
A
Sano
Hirokazu
H
Hikono
Tomoyuki
T
Shibahara
Yukio
Y
Yagi
Yoshihiro
Y
Shimoji
D018829:Adhesins, Bacterial / Q000276:immunology*; D000284:Administration, Oral; D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood / Q000276:immunology; D001428:Bacterial Vaccines / Q000276:immunology*; D004888:Erysipelothrix / Q000276:immunology* / Q000302:isolation & purification; D005260:Female; D055104:Infusions, Subcutaneous; D008168:Lung / Q000382:microbiology / Q000473:pathology; D051379:Mice; D045705:Mycoplasma hyopneumoniae / Q000276:immunology; D014066:Palatine Tonsil / Q000276:immunology / Q000382:microbiology; D045729:Pneumonia of Swine, Mycoplasmal / Q000276:immunology / Q000517:prevention & control*; D013552:Swine; D014613:Vaccines, Attenuated / Q000276:immunology
D018829:Adhesins, Bacterial; D000907:Antibodies, Bacterial; D001428:Bacterial Vaccines; C092236:P97 protein, Mycoplasma; D014613:Vaccines, Attenuated