Phosphoglycerate mutase in mammalian striated muscles: subcellular localization and binding partners.
Contrary to previously published data, we have found that in mammalian skeletal muscles, phosphoglycerate mutase (PGM) is organized in a regular, striated fashion within the sarcomere. In the absence of the enzyme effectors, PGM localizes mainly at the M-line, but under conditions typical for contracting muscle, the enzyme accumulates within the I-band of the sarcomere. Searching for muscle PGM binding partners, we have found that PGM interacts with several enzymes of triose phosphate metabolism. It might suggest that PGM is a central structural element of the muscle glycolytic complex located within the isotropic region of the sarcomere.
Department of Animal Molecular Physiology, Wroclaw University, Wroclaw, Poland.
Wojciech
W
Kowalski
Agnieszka
A
Gizak
Darek
D
Rakus
D000818:Animals; D002118:Calcium / Q000494:pharmacology; D005943:Gluconeogenesis; D005988:Glyceric Acids / Q000494:pharmacology; D006019:Glycolysis; D066298:In Vitro Techniques; D009097:Multienzyme Complexes / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D054792:Muscle, Striated / Q000187:drug effects / Q000201:enzymology*; D010736:Phosphoglycerate Mutase / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D011817:Rabbits; D051381:Rats; D012518:Sarcomeres / Q000187:drug effects / Q000201:enzymology; D013347:Subcellular Fractions / Q000187:drug effects / Q000201:enzymology
D005988:Glyceric Acids; D009097:Multienzyme Complexes; C005156:3-phosphoglycerate; D010736:Phosphoglycerate Mutase; D002118:Calcium