The effect of β-glucan on trace element levels in intra-abdominal sepsis in rats.
Sepsis is associated with the development of progressive damage in multiple organ systems. The beneficial effect of glucans has been attributed to modulation of immune function and enhances defense against bacterial, viral, fungal, and parasitic infections. The aim of this study was to investigate the putative protective effect of ß-glucan on changes of trace element levels in various tissues after experimental sepsis in rats. Sepsis was induced by cecal ligation and perforation (CLP) in 28 male Wistar albino rats. To evaluate this, rats were divided into four groups as sham operated, ß-glucan treated sham operated, CLP, and ß-glucan treated CLP. Sixteen hours after operation, rats were decapitated and zinc (Zn) and copper (Cu) levels were determined in the liver, kidney, heart, diaphragm, and lung tissues. The results demonstrate that sepsis significantly decreased zinc and copper levels of all tissues. The decrease in tissue zinc and copper levels demonstrates the role of trace elements in sepsis-induced tissue damage. Our results indicated that ß-glucan administration did not return the zinc and copper levels to the control group level, and it seems likely that the given dose of ß-glucan was insufficient to prevent sepsis-induced organ injury.
Department of Biophysics, Cerrahpasa Medical Faculty, Istanbul University, Istanbul, Turkey. hsf@istanbul.edu.tr
Semra
S
Ozdemir
Selmin
S
Toplan
Meltem
M
Ercan
Ibrahim
I
Bayrak
Bülent
B
Uruluer
Oguzhan
O
Sunamak
D000818:Animals; D003300:Copper / Q000378:metabolism; D003964:Diaphragm / Q000187:drug effects / Q000378:metabolism; D006321:Heart / Q000187:drug effects; D007416:Intestinal Perforation; D007668:Kidney / Q000187:drug effects / Q000378:metabolism; D008026:Ligation; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008168:Lung / Q000187:drug effects / Q000378:metabolism; D008297:Male; D009206:Myocardium / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D018805:Sepsis / Q000188:drug therapy* / Q000378:metabolism*; D014131:Trace Elements / Q000378:metabolism*; D015032:Zinc / Q000378:metabolism; D047071:beta-Glucans / Q000627:therapeutic use*
D014131:Trace Elements; D047071:beta-Glucans; D003300:Copper; D015032:Zinc