Inhibition of cell proliferation and glucose uptake in human laryngeal carcinoma cells by antisense oligonucleotides against glucose transporter-1.
BACKGROUND
Malignant cells show increased glucose uptake in vitro and in vivo, which is thought to be mediated by glucose transporters. In this study, we investigated the effect of plasmid-derived antisense RNA against the Glut-l gene on proliferation and glucose uptake in laryngeal carcinoma Hep-2 cells.


METHODS
The expression plasmids pcDNA3.1(+)-Glut-1 and pcDNA3.1(+)-anti Glut-1 were constructed. The MTT method was used to assess cell growth inhibition. The expression of Glut-1 mRNA and protein was detected by reverse transcriptase-polymerase chain reaction and Western blotting, respectively.


RESULTS
After transfection, Glut-1 AS clearly inhibited glucose uptake and cell growth in Hep-2 cells, and we observed a decrease in the expression of Glut-1 mRNA and protein in Hep-2 cells.


CONCLUSIONS
Glut-1 AS decreases glucose uptake and inhibits the proliferation of Hep-2 cells.
Department of Otolaryngology, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, China.
Shui-Hong
SH
Zhou
Jun
J
Fan
Xiao-Ming
XM
Chen
Ke-Jia
KJ
Cheng
Shen-Qing
SQ
Wang
D015153:Blotting, Western; D045744:Cell Line, Tumor / Q000166:cytology / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects*; D005947:Glucose / Q000493:pharmacokinetics*; D051272:Glucose Transporter Type 1 / Q000187:drug effects / Q000378:metabolism*; D006801:Humans; D007822:Laryngeal Neoplasms / Q000378:metabolism* / Q000473:pathology*; D016376:Oligonucleotides, Antisense / Q000494:pharmacology*; D010957:Plasmids / Q000187:drug effects / Q000378:metabolism; D016133:Polymerase Chain Reaction; D011336:Probability; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014162:Transfection
D051272:Glucose Transporter Type 1; D016376:Oligonucleotides, Antisense; D005947:Glucose