Tissue-specific mRNA expression profiles of drug-metabolizing enzymes and transporters in the cynomolgus monkey.
Pairs of forward and reverse primers and TaqMan probes specific to each of 19 drug-metabolizing enzymes (cytochrome P450s, UDP-glucuronosyltransferases, glutathione S-transferases, and sulfotransferases) and 5 transporters (ABC and SLC transporters) in the cynomolgus monkey were prepared. The expression level of each target mRNA was analyzed in total RNA obtained from three specimens of various cynomolgus monkey tissues (adrenal gland, brain, heart, kidney, large intestine, liver, lung, pancreas, prostate, small intestine, spleen, testis, and thymus) by real-time reverse transcription PCR using an Applied Biosystems 7500 Fast Real-Time PCR System. The data obtained in the present study provide useful information on tissue-specific profiles of the expression of these target mRNAs in the cynomolgus monkey, and the results are expected to be valuable in establishing drug metabolism- and transporter-mediated screening systems using the cynomolgus monkey for the evaluation of new chemical entities in new drug development.
Nishimura
Masuhiro
M
Research and Development Center, Otsuka Pharmaceutical Factory, Inc., Tokushima, Japan.
Koeda
Akiko
A
Morikawa
Hiroshi
H
Satoh
Tetsuo
T
Narimatsu
Shizuo
S
Naito
Shinsaku
S
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1 / Q000378:metabolism; D018528:ATP-Binding Cassette Transporters / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D002478:Cells, Cultured; D051544:Cytochrome P-450 CYP3A / Q000378:metabolism; D015342:DNA Probes; D004305:Dose-Response Relationship, Drug; D015870:Gene Expression; D020869:Gene Expression Profiling; D005982:Glutathione Transferase / Q000235:genetics / Q000378:metabolism; D008658:Inactivation, Metabolic; D008252:Macaca fascicularis; D008297:Male; D026901:Membrane Transport Proteins / Q000378:metabolism; D008862:Microsomes, Liver / Q000201:enzymology; D012333:RNA, Messenger / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013045:Species Specificity; D014018:Tissue Distribution
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D018528:ATP-Binding Cassette Transporters; D015342:DNA Probes; D026901:Membrane Transport Proteins; D012333:RNA, Messenger; D051544:Cytochrome P-450 CYP3A; D005982:Glutathione Transferase