Growth and characterization of high-quality protein crystals for X-ray crystallography.
Tetragonal hen egg white lysozyme is grown by the batch method in solution and gel media to study the influence of high magnetic fields on the quality of macromolecular crystals. The crystallographic quality of crystals grown in the absence and in the presence of 7- and 10-T fields are analyzed in terms of mosaicity and high-resolution X-ray imaging methods. Crystals grown by the batch method from solution showed a remarkable enhancement of the crystallographic quality, although the overall crystal quality was higher for gel-grown crystals than solution-grown crystals. The observed improvement in crystal quality can be attributed to the suppression of convective transport during the crystal growth process and the control of the nucleation kinetics by the use of a magnetic force.
Moreno
Abel
A
Instituto de Química, Universidad Nacional Autónoma de México, México City, Mexico.
Yokaichiya
Fabiano
F
Dimasi
Elaine
E
Stojanoff
Vivian
V
D000818:Animals; D002645:Chickens; D003460:Crystallization / Q000379:methods*; D018360:Crystallography, X-Ray / Q000379:methods*; D005782:Gels / Q000737:chemistry; D009113:Muramidase / Q000032:analysis* / Q000737:chemistry*; D044367:Phase Transition
D005782:Gels; D009113:Muramidase