Identification of ORF636 in phage phiSLT carrying Panton-Valentine leukocidin genes, acting as an adhesion protein for a poly(glycerophosphate) chain of lipoteichoic acid on the cell surface of Staphylococcus aureus.
The temperate phage phiSLT of Staphylococcus aureus carries genes for Panton-Valentine leukocidin. Here, we identify ORF636, a constituent of the phage tail tip structure, as a recognition/adhesion protein for a poly(glycerophosphate) chain of lipoteichoic acid on the cell surface of S. aureus. ORF636 bound specifically to S. aureus; it did not bind to any other staphylococcal species or to several gram-positive bacteria.
Kaneko
Jun
J
Department of Microbial Biotechnology, Graduate School of Agricultural Science, Tohoku University, Aoba-ku, Sendai, Japan. jkaneko@biochem.tohoku.ac.jp
Narita-Yamada
Sachiko
S
Wakabayashi
Yukari
Y
Kamio
Yoshiyuki
Y
D000595:Amino Acid Sequence; D001427:Bacterial Toxins / Q000235:genetics; D005098:Exotoxins / Q000235:genetics; D005994:Glycerophosphates / Q000378:metabolism*; D007956:Leukocidins / Q000235:genetics; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D011485:Protein Binding; D016415:Sequence Alignment; D013204:Staphylococcus Phages / Q000235:genetics* / Q000502:physiology; D013211:Staphylococcus aureus / Q000821:virology*; D020349:Surface Plasmon Resonance; D010948:Viral Plaque Assay; D017376:Viral Tail Proteins / Q000235:genetics* / Q000378:metabolism; D053585:Virus Attachment
D001427:Bacterial Toxins; D005098:Exotoxins; D005994:Glycerophosphates; D007956:Leukocidins; C078284:Panton-Valentine leukocidin; D017376:Viral Tail Proteins