Flash-induced structural dynamics in photosystem II membrane fragments of green plants.
Time-resolved quasielastic neutron scattering with laser excitation is a promising novel pump-probe approach, which opens up new perspectives for the study of protein-membrane dynamics in specific functional states of even complex systems. This is demonstrated here for the case of photosystem II membrane fragments with inhibited electron transfer. In contrast to the case of the model system bacteriorhodopsin, a transient reduction of the dynamics is observed approximately 160 micros after the actinic laser flash. This effect is the first observation of a modulated structural dynamics in photosystem II membrane fragments.
Pieper
Jörg
J
Max-Volmer-Laboratories for Biophysical Chemistry, Technische Universitat Berlin, Strasse des 17. Juni 135, 10623 Berlin, Germany. joerg.pieper@tu-berlin.de
Renger
Gernot
G
D000465:Algorithms; D004735:Energy Transfer; D007834:Lasers; D008027:Light; D033363:Neutron Diffraction; D045332:Photosystem II Protein Complex / Q000737:chemistry* / Q000528:radiation effects*; D011487:Protein Conformation / Q000528:radiation effects; D013050:Spectrometry, Fluorescence; D018724:Spinacia oleracea / Q000201:enzymology*; D020524:Thylakoids / Q000737:chemistry / Q000528:radiation effects
D045332:Photosystem II Protein Complex