Live cell labeling of glial progenitor cells using targeted quantum dots.
This study describes the development of targeted quantum dots (T-QDs) as biomarkers for the labeling of glial progenitor cells (GPCs) that over express platelet derived growth factor (PDGF) and its receptor PDGFR (GPC(PDGF)). PDGFR plays a critical role in glioma development and growth, and is also known to affect multiple biological processes such as cell migration and embryonic development. T-QDs were developed using streptavidin-conjugated quantum dots (S-QDs) with biotinylated antibodies and utilized to label the intracellular and extracellular domains of live, cultured GPC(PDGF) cells via lipofection with cationic liposomes. Confocal studies illustrate successful intracellular and extracellular targeted labeling within live cells that does not appear to impact upstream PDGFR dynamics during real-time signaling events. Further, T-QDs were nontoxic to GPC(PDGF) cells, and did not alter cell viability or proliferation over the course of 6 days. These results raise new applications for T-QDs as ultra sensitive agents for imaging and tracking of protein populations within live cells, which that will enable future mechanistic study of oncogenic signaling events in real-time.
Department of Biomedical Engineering, The City College of The City University of New York, New York, NY 10031, USA.
Nidhi
N
Sabharwal
Eric C
EC
Holland
Maribel
M
Vazquez
D000911:Antibodies, Monoclonal / Q000032:analysis*; D002478:Cells, Cultured; D006801:Humans; D015336:Molecular Probe Techniques; D009457:Neuroglia / Q000166:cytology / Q000378:metabolism*; D010982:Platelet-Derived Growth Factor / Q000032:analysis*; D045663:Quantum Dots; D013050:Spectrometry, Fluorescence / Q000379:methods*; D013194:Staining and Labeling / Q000379:methods; D013234:Stem Cells / Q000166:cytology / Q000378:metabolism*
D000911:Antibodies, Monoclonal; D010982:Platelet-Derived Growth Factor