Pollen grain development is compromised in Arabidopsis agp6 agp11 null mutants.
Arabinogalactan proteins (AGPs) are structurally complex plasma membrane and cell wall proteoglycans that are implicated in diverse developmental processes, including plant sexual reproduction. Male gametogenesis (pollen grain development) is fundamental to plant sexual reproduction. The role of two abundant, pollen-specific AGPs, AGP6, and AGP11, have been investigated here. The pollen specificity of these proteoglycans suggested that they are integral to pollen biogenesis and their strong sequence homology indicated a potential for overlapping function. Indeed, single gene transposon insertion knockouts for both AGPs showed no discernible phenotype. However, in plants homozygous for one of the insertions and heterozygous for the other, in homozygous double mutants, and in RNAi and amiRNA transgenic plants that were down-regulated for both genes, many pollen grains failed to develop normally, leading to their collapse. The microscopic observations of these aborted pollen grains showed a condensed cytoplasm, membrane blebbing and the presence of small lytic vacuoles. Later in development, the generative cells that arise from mitotic divisions were not seen to go into the second mitosis. Anther wall development, the establishment of the endothecium thickenings, the opening of the stomium, and the deposition of the pollen coat were all normal in the knockout and knockdown lines. Our data provide strong evidence that these two proteoglycans have overlapping and important functions in gametophytic pollen grain development.
Coimbra
Sílvia
S
Departamento de Botânica, Faculdade de Ciências, Universidade do Porto, Edifício FC4 Rua do Campo Alegre 4169-007 Porto, Portugal. scoimbra@fc.up.pt
Costa
Mário
M
Jones
Brian
B
Mendes
Marta Adelina
MA
Pereira
Luís Gustavo
LG
D017360:Arabidopsis / Q000235:genetics* / Q000254:growth & development* / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D020868:Gene Silencing; D016254:Mutagenesis, Insertional; D011058:Pollen / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D011509:Proteoglycans / Q000235:genetics / Q000378:metabolism*
C544319:AGP11 protein, Arabidopsis; C544318:AGP6 protein, Arabidopsis; D029681:Arabidopsis Proteins; D011509:Proteoglycans