Reactive oxygen species production is increased in the peripheral blood monocytes of obese patients.
Infiltrating macrophages play an important role in the production of inflammatory mediators by the adipose tissue of obese subjects. To reach the adipose tissue, peripheral monocytes are recruited by locally produced chemoattractants. However, little is known about the activation of monocytes in the peripheral blood of obese subjects. The objective of this study was to determine reactive oxygen species and endoplasmic reticulum stress as early markers of monocytic commitment with an inflammatory phenotype in the peripheral blood of nondiabetic obese patients. Patients were recruited from an academic general hospital; controls were voluntary students. Seven lean controls and 6 nondiabetic obese patients were included in the study. Monocytes were prepared from peripheral blood. Immunoblot, flow cytometry, and polymerase chain reaction were used to determine reactive oxygen species and endoplasmic reticulum stress. Increased reactive oxygen species and activation of endoplasmic reticulum stress were detected in the monocytes from obese patients. Reducing endoplasmic reticulum stress with a chemical chaperone reversed monocytic activation, as determined by the reduction of reactive oxygen species production. Thus, monocytes from nondiabetic obese patients are already committed with an inflammatory phenotype in peripheral blood; and reducing endoplasmic reticulum stress negatively modulates their activation.
Department of Internal Medicine, University of Campinas, Campinas-SP 13084-970, Brazil.
Giovanna R
GR
Degasperi
Raphael G P
RG
Denis
Joseane
J
Morari
Carina
C
Solon
Bruno
B
Geloneze
Christiane
C
Stabe
José Carlos
JC
Pareja
Aníbal E
AE
Vercesi
Lício A
LA
Velloso
D000328:Adult; D002118:Calcium / Q000378:metabolism; D002374:Catalase / Q000378:metabolism; D003600:Cytosol / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D004721:Endoplasmic Reticulum / Q000378:metabolism*; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D015151:Immunoblotting; D047468:Immunoprecipitation; D007249:Inflammation / Q000097:blood / Q000378:metabolism*; D008297:Male; D009000:Monocytes / Q000201:enzymology / Q000378:metabolism*; D009765:Obesity / Q000097:blood*; D018384:Oxidative Stress; D010641:Phenotype; D016133:Polymerase Chain Reaction; D012326:RNA Splicing; D012333:RNA, Messenger / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000097:blood*; D000071701:Regulatory Factor X Transcription Factors; D013482:Superoxide Dismutase / Q000378:metabolism; D014157:Transcription Factors / Q000378:metabolism
D004268:DNA-Binding Proteins; D012333:RNA, Messenger; D017382:Reactive Oxygen Species; D000071701:Regulatory Factor X Transcription Factors; D014157:Transcription Factors; D002374:Catalase; D013482:Superoxide Dismutase; D002118:Calcium