Scanning all-fiber-optic endomicroscopy system for 3D nonlinear optical imaging of biological tissues.
An extremely compact all-fiber-optic scanning endomicroscopy system was developed for two-photon fluorescence (TPF) and second harmonic generation (SHG) imaging of biological samples. A conventional double-clad fiber (DCF) was employed in the endomicroscope for single-mode femtosecond pulse delivery, multimode nonlinear optical signals collection and fast two-dimensional scanning. A single photonic bandgap fiber (PBF) with negative group velocity dispersion at two-photon excitation wavelength (i.e. approximately 810 nm) was used for pulse prechirping in replacement of a bulky grating/lens-based pulse stretcher. The combined use of DCF and PBF in the endomicroscopy system made the endomicroscope basically a plug-and-play unit. The excellent imaging ability of the extremely compact all-fiber-optic nonlinear optical endomicroscopy system was demonstrated by SHG imaging of rat tail tendon and depth-resolved TPF imaging of epithelial tissues stained with acridine orange. The preliminary results suggested the promising potential of this extremely compact all-fiber-optic endomicroscopy system for real-time assessment of both epithelial and stromal structures in luminal organs.
Wu
Yicong
Y
Department of Bioengineering, University of Washington, Seattle, WA 98195, USA.
Leng
Yuxin
Y
Xi
Jiefeng
J
Li
Xingde
X
D000818:Animals; D003315:Cornea / Q000166:cytology; D005336:Fiber Optic Technology / Q000379:methods*; D005453:Fluorescence; D021621:Imaging, Three-Dimensional / Q000379:methods*; D008853:Microscopy / Q000379:methods*; D055095:Optics and Photonics / Q000379:methods*; D017785:Photons; D051381:Rats; D034421:Sus scrofa