The fluorescence enhancement of quercetin-nucleic acid system and the analytical application.
Nucleic acid can greatly enhance the fluorescence intensity of quercetin in HMTA-HCl (pH 5.5) buffer. The enhanced intensity is in proportion to the concentration of nucleic acids in the range 5.0 x 10(-9) to 1.0 x 10(-6) g/mL for fsDNA, 5.0 x 10(-9) to 7.0 x 10(-7) g/mL for ctDNA and 5.0 x 10(-9) to 1.0 x 10(-6) g/mL for yRNA, and their detection limits (S/N = 3) are 3.5 x 10(-9), 7.8 x 10(-10) and 2.6 x 10(-9) g/mL, respectively. In comparison with most reported fluorescent probes for the determination of nucleic acids, the proposed probe has higher sensitivity and lower toxicity. The interaction investigation indicates that quercetin binds with double-strand DNA in groove binding mode, resulting in fluorescence enhancement of this system.
Key Laboratory of Colloid and Interface Chemistry (Shandong University), Ministry of Education, School of Chemistry and Chemical Engineering, Shandong University, Jinan 250100, People's Republic of China.
Yuan
Y
Liu
Xia
X
Wu
Haiping
H
Zhou
Xiaoyu
X
Liu
Fengju
F
Zhang
Jinghe
J
Yang
D005456:Fluorescent Dyes / Q000737:chemistry*; D057230:Limit of Detection; D015394:Molecular Structure; D009696:Nucleic Acids / Q000032:analysis* / Q000737:chemistry; D011794:Quercetin / Q000737:chemistry*; D013050:Spectrometry, Fluorescence
D005456:Fluorescent Dyes; D009696:Nucleic Acids; D011794:Quercetin