Colloidal crystal beads composed of core-shell particles for multiplex bioassay.
A convenient method was developed to fabricate colloidal crystal beads (CCBs) with tough mechanical strength, which was used as encoded carriers for multiplex bioassay. The latex particles used for the construction of the CCBs were designed with a rigid core PS and a elastomeric shell poly(MMA/EA/MAA), and were prepared via one-step soap-free emulsion polymerization. The as-above-prepared CCBs were thermo-treated to drive the elastomeric shells of adjacent latex particles joining together. It was found that the coalescence of latex particles can greatly improve the mechanical strength of the CCBs for multiplex bioassay.
State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, China.
Hua
H
Xu
Cun
C
Zhu
Yuanjin
Y
Zhao
Xiangwei
X
Zhao
Jing
J
Hu
Zhongze
Z
Gu
D007118:Immunoassay / Q000379:methods*; D008855:Microscopy, Electron, Scanning; D008856:Microscopy, Fluorescence; D008863:Microspheres; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure*; D011109:Polymethacrylic Acids / Q000138:chemical synthesis* / Q000737:chemistry; D011137:Polystyrenes / Q000138:chemical synthesis* / Q000737:chemistry; D013696:Temperature
D011109:Polymethacrylic Acids; D011137:Polystyrenes; C541067:poly(styrene-methyl methacrylate-ethyl acrylate-methacrylic acid)