Effects of pH on transport properties of articular cartilages.
Articular cartilages swell and shrink depending on the ionic strength of the electrolyte they are in contact with. This electro-chemo-mechanical coupling is due to the presence of fixed electrical charges on proteoglycans (PGs). In addition, at nonphysiological pH, collagen fibers become charged. Therefore, variation of the pH of the electrolyte has strong implications on the electrical charge of cartilages and, by the same token, on their transport and mechanical properties. Articular cartilages are viewed as three-phase multi-species porous media. The constitutive framework is phrased in the theory of thermodynamics of porous media. Acid-base reactions, as well as calcium binding, are embedded in this framework. Although macroscopic in nature, the model accounts for a number of biochemical details defining collagen and PGs. The change of the electrical charge is due to the binding of hydrogen ions on specific sites of PGs and collagen. Simulations are performed mimicking laboratory experiments where either the ionic strength or the pH of the bath, the cartilage piece is in contact with, is varied. They provide the evolutions of the chemical compositions of mobile ions, of the sites of acid-base reactions and calcium binding, and of the charges of collagen and glycosaminoglycans, at constant volume fraction of water. Emphasis is laid on the effects of pH, ionic strength and calcium binding on the transport properties of cartilages, and, in particular, on the electrical conductivity and electro-osmotic coefficient.
Loret
Benjamin
B
Laboratoire Sols, Solides, Structures, Institut National Polytechnique de Grenoble, B.P. 53X, 38041, Grenoble Cedex, France. benjamin.loret@inpg.fr
Simões
Fernando M F
FM
D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D002118:Calcium / Q000378:metabolism; D002122:Calcium Chloride / Q000494:pharmacology; D002358:Cartilage, Articular / Q000187:drug effects / Q000378:metabolism*; D002417:Cattle; D003094:Collagen / Q000378:metabolism; D003198:Computer Simulation; D004058:Diffusion / Q000187:drug effects; D004560:Electricity; D004573:Electrolytes; D006801:Humans; D006863:Hydrogen-Ion Concentration / Q000187:drug effects; D009994:Osmolar Concentration; D011509:Proteoglycans / Q000378:metabolism; D012965:Sodium Chloride / Q000494:pharmacology
D004573:Electrolytes; D011509:Proteoglycans; D012965:Sodium Chloride; D003094:Collagen; D002122:Calcium Chloride; D002118:Calcium