Effects of trxS gene on protein degradation in germinating barley seeds.
We assessed the effects of trxS gene on changes of proteinase activity, contents of different protein fractions and SDS-PAGE profiles in germinating seeds of contrasting transgenic and nontransgenic barley variety. Proteinase activity was enhanced by 70.28% in transgenic than nontransgenic barley seeds, whereas contents of albumin, globulin, hordein and glutelin in transgenic seeds were 3.68%, 23.52%, 31.37%, and 21.04%, lower than those in nontransgenic seeds. Degradation rates of hordein and glutelin in transgenic seeds were faster than those in nontransgenic seedlings as indicated by the SDS-PAGE profiles. Our data imply that the transformation of trxS gene could promote the degradation of protein, providing theoretic basis for the use of trxS gene and barley quality breeding.
Henan Agricultural University, Zhengzhou 450002, China.
Li
L
Wei
Weiwei
W
Kong
Jun
J
Yin
Weijie
W
Wang
D018525:Germination; D001467:Hordeum / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism*; D010447:Peptide Hydrolases / Q000378:metabolism; D010940:Plant Proteins / Q000378:metabolism*; D030821:Plants, Genetically Modified / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D012639:Seeds / Q000254:growth & development*; D013879:Thioredoxins / Q000235:genetics / Q000378:metabolism*; D014170:Transformation, Genetic
D010940:Plant Proteins; D013879:Thioredoxins; D010447:Peptide Hydrolases