Intestinal microbiota in exclusively breast-fed infants with blood-streaked stools.
Intestinal microbiota in exclusively breast-fed infants with blood-streaked stools and in healthy exclusively breast-fed babies was compared. Total anaerobes, bifidobacteria, lactobacilli, coliform bacteria, enterococci and clostridia were quantified by cultivation methods in feces of 17 full-term exclusively breastfed patients (aged 16.3 +/- 7.4 weeks) with blood-streaked stools and in the control group of 22 healthy fullterm exclusively breast-fed infants (13.7 +/- 6.4 weeks). Specific fluorescence in situ hybridization kits for Bifidobacterium spp. were used for the quantitative detection of bifidobacteria in samples. Control samples had significantly (p < 0.05) higher counts of total anaerobes. Bifidobacteria were not detected in patients' samples in 65 % and in controls in 36 % (p < 0.01). Bifidobacteria counts were also significantly higher in the control group (p < 0.01). Furthermore, clostridia strains were detected only in feces from bifidobacteria-negative infants reaching counts >8 log CFU/g. Lactobacilli were not detected in 65 % patients and in 45 % control samples. However, this difference was not significant as well as the difference in lactobacilli counts. Eosinophilia was observed in 35 % of patients, low IgA concentration in 71 % and also low IgG concentration in 71 %. pANCA positivity was found in 53 % of patients. In conclusion a significant low proportion of bifidobacterial microbiota in patients with blood-streaked stools was shown in comparison with controls.
Department of Pediatrics, 2nd Medical School of the Charles University in Prague and University Hospital Motol, 150 06, Prague, Czech Republic. jiri.nevoral@seznam.cz
J
J
Nevoral
V
V
Rada
E
E
Vlková
K
K
Bláhová
J
J
Bronský
D
D
Bubáková
J
J
Killer
D001419:Bacteria / Q000302:isolation & purification*; D001942:Breast Feeding; D005243:Feces / Q000382:microbiology*; D005260:Female; D006801:Humans; D007070:Immunoglobulin A / Q000097:blood; D007074:Immunoglobulin G / Q000097:blood; D007223:Infant; D007422:Intestines / Q000382:microbiology*; D008297:Male; D011350:Proctocolitis / Q000276:immunology / Q000382:microbiology*
D007070:Immunoglobulin A; D007074:Immunoglobulin G