Identification of a new broad-spectrum CD8+ T cell epitope from over-expressed antigen COX-2 in esophageal carcinoma.
Cyclooxygenase-2 (COX-2) has been found to be over-expressed in esophageal carcinoma (EC) and it could be considered as a potential tumor-associated antigen (TAA). In the present study, six candidate peptides from COX-2 were firstly predicted and synthesized. Among them, P(479) had the highest affinity and stability toward both HLA-A *0201 and HLA-A *03 molecules and it could significantly promote the IFN-gamma release. The cytotoxic T lymphocytes (CTLs) induced by P(479) could specifically lyse COX-2-expressed EC cell lines, EC-1 (HLA-A3 supertype) and EC-9706 (HLA-A2 supertype). These results suggested that P(479) as a novel broad-spectrum T cell epitope would be very useful in immunotherapy against esophageal carcinoma.
Gao
Yan-feng
YF
Department of Bioengineering, Zhengzhou University, Zhengzhou 450001, China.
Sun
Zhan-qiang
ZQ
Qi
Feng
F
Qi
Yuan-ming
YM
Zhai
Ming-xia
MX
Lou
Hui-ping
HP
Chen
Li-xiang
LX
Li
Yong-xin
YX
Wang
Xian-yuan
XY
D000951:Antigens, Neoplasm / Q000096:biosynthesis* / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D045744:Cell Line, Tumor; D051546:Cyclooxygenase 2 / Q000096:biosynthesis* / Q000276:immunology; D003602:Cytotoxicity, Immunologic; D018984:Epitopes, T-Lymphocyte; D004938:Esophageal Neoplasms / Q000276:immunology* / Q000473:pathology; D015234:HLA-A Antigens / Q000276:immunology; D015789:HLA-A2 Antigen; D015791:HLA-A3 Antigen; D006801:Humans; D007371:Interferon-gamma / Q000096:biosynthesis; D009842:Oligopeptides / Q000138:chemical synthesis / Q000276:immunology / Q000494:pharmacology*
D000951:Antigens, Neoplasm; D018984:Epitopes, T-Lymphocyte; D015234:HLA-A Antigens; C435939:HLA-A*02:01 antigen; C487960:HLA-A*03 antigen; D015789:HLA-A2 Antigen; D015791:HLA-A3 Antigen; D009842:Oligopeptides; D007371:Interferon-gamma; D051546:Cyclooxygenase 2