[The role of CyPA in chemotaxis of neutrophil in rheumatoid arthritis and secretion of interleukin-8].
OBJECTIVE
To ascertain the effect of CyPA and IL-8 in chemotaxis of neutrophil and the level of IL-8 from the CyPA effecting of peripheral blood from RA patients.


METHODS
12 RA patients matched 4 healthy people were studied. Chemotaxis of IL-8 was measured by Boyden chamber on neutrophil of RA patients and that of 4 normal healthy people controls were studied; the level of IL-8 on neutrophil of RA patients peripheral blood after the effecting of CyPA was assessed by ELISA. Correlations between CyPA and IL-8 were observed in RA.


RESULTS
The chemotaxis of neutrophil which IL-8 mixed with CyPA antibody was lower than IL-8(P<0.05); The secretion of RA peripheral blood in IL-8 was higher than normal people, as well as the secretion of RA peripheral blood in IL-8 after effecting of CyPA was higher than before (P<0.05), but the level of IL-8 after blocking CyPA was not changed.


CONCLUSIONS
CyPA could affect the chemotaxis of IL-8 in the neutrophil of RA patients' peripheral blood. The secretion of IL-8 is accelerated by CyPA on neutrophil of RA patients'peripheral blood.
Department of Clinical Immunology, Xijing Hospital, Fourth Military Medical University, Xi'an, China.
Bei
B
Zhang
Cong-hua
CH
Wang
Yan-hong
YH
Wang
Chun-mei
CM
Fan
Ping
P
Zhu
D000328:Adult; D000368:Aged; D000911:Antibodies, Monoclonal / Q000494:pharmacology; D001172:Arthritis, Rheumatoid / Q000097:blood* / Q000473:pathology; D002633:Chemotaxis / Q000187:drug effects*; D017077:Culture Media, Conditioned / Q000378:metabolism; D021984:Cyclophilin A / Q000276:immunology / Q000494:pharmacology*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D018922:HL-60 Cells; D006801:Humans; D016209:Interleukin-8 / Q000378:metabolism* / Q000494:pharmacology; D008297:Male; D008875:Middle Aged; D009504:Neutrophils / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism
D000911:Antibodies, Monoclonal; D017077:Culture Media, Conditioned; D016209:Interleukin-8; D021984:Cyclophilin A