Inhibitory mechanism of saponins derived from roots of Platycodon grandiflorum on anaphylactic reaction and IgE-mediated allergic response in mast cells.
The purpose of this study was to investigate the protective effects of saponins isolated from the root of Platycodi Radix (Changkil saponins: CKS) anti-allergic effects in mice and mast cells. Oral administration of CKS inhibited the dinitrophenyl (DNP)-IgE antibody-induced systemic PCA reaction in mice. CKS reduced the beta-hexosaminidase and histamine release from anti-DNP-IgE-sensitized RBL-2H3 cells. In addition, CKS inhibited the IgE antibody-induced increases in IL-4 and TNF-alphaproduction and expression in RBL-2H3 cells. In order to explore the inhibitory mechanism of CKS in PCA and mast cell degranulation, we examined the activation of intracellular signaling molecules. CKS suppressed DNP-IgE antibody-induced Syk phosphorylation. Further downstream, CKS also inhibited the phosphorylation of Akt and MAP kinases. Taken together, the in vivo/in vitro anti-allergic effects of CKS suggest possible therapeutic applications for this agent in allergic diseases through the inhibition of inflammatory cytokines and Syk-dependent signaling cascades.
Han
Eun Hee
EH
BK21 Project Team, Department of Pharmacy, College of Pharmacy, Chosun University, 375 Seosuk-dong, Gwangju 501-759, South Korea.
Park
Jin Hee
JH
Kim
Ji Young
JY
Chung
Young Chul
YC
Jeong
Hye Gwang
HG
D000707:Anaphylaxis / Q000276:immunology*; D000818:Animals; D018926:Anti-Allergic Agents; D015153:Blotting, Western; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D016207:Cytokines / Q000096:biosynthesis; D005260:Female; D006596:Hexosaminidases / Q000378:metabolism; D006636:Histamine Release / Q000187:drug effects; D006967:Hypersensitivity / Q000276:immunology*; D007073:Immunoglobulin E / Q000276:immunology*; D008156:Luciferases / Q000235:genetics; D008407:Mast Cells / Q000276:immunology*; D051379:Mice; D008813:Mice, Inbred ICR; D010323:Passive Cutaneous Anaphylaxis / Q000187:drug effects; D018517:Plant Roots / Q000737:chemistry; D031238:Platycodon / Q000737:chemistry*; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012503:Saponins / Q000302:isolation & purification / Q000494:pharmacology*; D013347:Subcellular Fractions / Q000187:drug effects; D014162:Transfection
D018926:Anti-Allergic Agents; D016207:Cytokines; D012333:RNA, Messenger; D012503:Saponins; D007073:Immunoglobulin E; D008156:Luciferases; D006596:Hexosaminidases