Atractylodes japonica root extract protects osteoblastic MC3T3-E1 cells against hydrogen peroxide-induced inhibition of osteoblastic differentiation.
The rhizome of Atractylodes japonica Koidz. has been used traditionally for the treatment of water retention in the body and is known to have estrogen-like activity. In the present study, the protective effects of A. japonica root extract on the response of osteoblasts to oxidative stress were evaluated. Osteoblastic MC3T3-E1 cells were incubated with hydrogen peroxide and/or A. japonica root extract, and markers of osteoblast function and oxidative damage were examined. A. japonica root extract significantly (p < 0.05) increased cell survival, alkaline phosphatase activity, collagen and calcium deposition and decreased the production of receptor activator of nuclear factor-kB ligand (RANKL), protein carbonyl and malondialdehyde of osteoblastic MC3T3-E1 cells in the presence of hydrogen peroxide. These results demonstrate that A. japonica root can protect the cells from oxidative stress-induced toxicity and may have positive effects on skeletal structure.
Choi
Eun Mi
EM
Plant Resources Research Institute, Duksung Women's University, Seoul 132-714, Korea.
Kim
Gun-Hee
GH
Lee
Yong Soo
YS
D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D031174:Atractylodes / Q000737:chemistry*; D002118:Calcium / Q000378:metabolism; D002454:Cell Differentiation / Q000187:drug effects*; D002460:Cell Line; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D015227:Lipid Peroxidation; D051379:Mice; D010006:Osteoblasts / Q000187:drug effects*; D018384:Oxidative Stress; D010936:Plant Extracts / Q000494:pharmacology*; D018517:Plant Roots / Q000737:chemistry; D053245:RANK Ligand / Q000378:metabolism
D010936:Plant Extracts; D053245:RANK Ligand; C505689:Tnfsf11 protein, mouse; D006861:Hydrogen Peroxide; D000469:Alkaline Phosphatase; D002118:Calcium