Pore formation kinetics in membranes, determined from the release of marker molecules out of liposomes or cells.
We discuss the efflux of entrapped marker material from liposomes or cells through pores in the membrane, being monitored by the time course of a certain signal F (e.g., fluorescence emission). This is expressed in terms of an appropriate normalized function of time, the so-called efflux function E(t). Under conditions frequently encountered in practice the measured E(t) can be easily related to the forward rate of pore formation if the liposomes/cells are monodisperse in size. In the basic case of a time-independent rate law it turns out that E(t) must be single exponential. Deviations from such a simple functional behavior might be due to a fairly broad distribution of liposome/cell sizes and/or a more complicated pore formation mechanism. A relevant evaluation of original data is demonstrated making use of experimental results obtained with small unilamellar lipid vesicles where pores are induced by the antibiotic peptide alamethicin. This includes the application of a general method to eliminate the effect of a given liposome/cell size distribution.
Department of Biophysical Chemistry, Biocenter of the University, CH-4056 Basel, Switzerland.
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Schwarz
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Robert