Novel application for isothermal nucleic acid sequence-based amplification (NASBA).
Human bocavirus (HBoV), solely based on phylogenetic analyses, was classified as the second autonomous human parvovirus. Unfortunately, neither susceptible cell cultures nor animal models were described hitherto, thus complicating studies on viral genome structure and replication steps. A novel application of nucleic acid sequence-based amplification (NASBA) revealed that in all tested samples (100%) that became positive by NASBA the negative strand of the HBoV genome was packaged. Additionally, two of those samples also contained a detectable amount of positive strand (14.3%). The data confirm the assumed single-stranded negative-sense nature of HBoV-genomes that is independent of the viral subtype while showing that NASBA is useful not only for diagnosis.
Institute of Virology, University of Bonn Medical Centre, Bonn, Germany.
Anne
A
Böhmer
Verena
V
Schildgen
Jessica
J
Lüsebrink
Susanne
S
Ziegler
Ramona L
RL
Tillmann
Michael
M
Kleines
Oliver
O
Schildgen
D052660:Bocavirus / Q000235:genetics* / Q000302:isolation & purification; D004277:DNA, Single-Stranded / Q000235:genetics*; D004279:DNA, Viral / Q000235:genetics*; D006801:Humans; D010322:Parvoviridae Infections / Q000821:virology; D021161:Self-Sustained Sequence Replication / Q000379:methods*; D014771:Virion / Q000235:genetics*
D004277:DNA, Single-Stranded; D004279:DNA, Viral