Analysis of pralidoxime in serum, brain and CSF of rats.
After administration of various amounts of pralidoxime to rats, the levels in serum, brain and cerebrospinal fluid (CSF) were measured using capillary zone electrophoresis (CZE). The calibration curves were established using spiked samples. The calibration covers the ranges from 0.3 - 200 microg/mL, 0.3 - 7 microg/mL and 0.1 - 7 microg/mL for serum, brain and CSF, respectively. The CZE measurement opens the way to the fast and reliable determination of pyridinium aldoxime concentrations in serum, cerebrospinal fluid and brain, thereby monitoring blood-brain and blood-CSF penetration of pyridinium aldoxime-type antidotes clinically used in organophosphate poisoning.
Kalász
Huba
H
Department of Pharmacology & Therapeutics, Faculty of Medicine & Health Sciences, United Arab Emirates University, Al Ain, United Arab Emirates. drkalasz@gmail.com
Szöko
Eva
E
Tábi
Tamás
T
Petroianu
Georg A
GA
Lorke
Dietrich E
DE
Omar
Abdulrab
A
Alafifi
Salem
S
Jasem
Almerri
A
Tekes
Kornélia
K
D000818:Animals; D000931:Antidotes / Q000032:analysis* / Q000493:pharmacokinetics; D001812:Blood-Brain Barrier / Q000378:metabolism; D001923:Brain Chemistry; D004305:Dose-Response Relationship, Drug; D019075:Electrophoresis, Capillary; D008297:Male; D011220:Pralidoxime Compounds / Q000032:analysis / Q000097:blood* / Q000134:cerebrospinal fluid* / Q000493:pharmacokinetics; D051381:Rats; D017208:Rats, Wistar
D000931:Antidotes; D011220:Pralidoxime Compounds; C028797:pralidoxime