Berry anthocyanins and their aglycons inhibit monoamine oxidases A and B.
Monoamine oxidases (MAO) are mitochondrial enzymes that catalyze the oxidation of monoamines in multiple tissues, including the brain. Elevated MAO activity has long been implicated in the etiology of depression, anxiety, and neurodegenerative disease, fuelling the search for inhibitors in the prevention and treatment of these disorders. We hypothesized that emerging neuroprotective effects of anthocyanins from berry fruits may be explained by an affinity of these polyphenols for MAO isoforms A or B. Using a luminometric MAO assay, 25 anthocyanidins, anthocyanidin-3-glycosides, anthocyanidin-3,5-diglucosides, proanthocyanidins, and phenolic metabolites were examined. For MAO A and B, IC(50) values in the low micromolar range were reached by anthocyanidins and anthocyanidin-3-glycosides, as opposed to values in the low millimolar range for phenolic acids. Kinetic analyses, performed with cyanidin and cyanidin-3-glucoside, indicated a competitive interaction of cyanidin with MAO A plus a mixed competitive and non-competitive mode of interaction of cyanidin with MAO B and of cyanidin-3-glucoside with both enzyme isoforms. Thus anthocyanins and their aglycons achieve MAO inhibition in vitro that is compatible with central nervous functionalities. For extrapolation of the present findings to in vivo effects, future studies will need to address in more detail the bioavailability of these dietary constituents.
Dreiseitel
Andrea
A
Department of Psychiatry, University of Regensburg, Franz-Josef-Strauss-Allee 11, 93053 Regensburg, Germany. andrea.dreiseitel@klinik.uni-regensburg.de
Korte
Gabriele
G
Schreier
Peter
P
Oehme
Anett
A
Locher
Sanja
S
Domani
Martina
M
Hajak
Goeran
G
Sand
Philipp G
PG
D000818:Animals; D000872:Anthocyanins / Q000737:chemistry / Q000494:pharmacology*; D002460:Cell Line; D005638:Fruit / Q000737:chemistry*; D006027:Glycosides / Q000737:chemistry / Q000494:pharmacology; D006801:Humans; D007527:Isoenzymes / Q000037:antagonists & inhibitors; D008995:Monoamine Oxidase / Q000378:metabolism*; D008996:Monoamine Oxidase Inhibitors / Q000737:chemistry / Q000494:pharmacology*; D018696:Neuroprotective Agents / Q000737:chemistry / Q000494:pharmacology*; D010636:Phenols / Q000737:chemistry / Q000494:pharmacology; D044945:Proanthocyanidins / Q000737:chemistry / Q000494:pharmacology
D000872:Anthocyanins; D006027:Glycosides; D007527:Isoenzymes; D008996:Monoamine Oxidase Inhibitors; D018696:Neuroprotective Agents; D010636:Phenols; D044945:Proanthocyanidins; D008995:Monoamine Oxidase