In-vitro radiosensitivity in patients with systemic lupus erythematosus.
To determine the "in-vitro" intrinsic cell radiosensitivity (RS) as a risk indicator of radiation-related side-effects in patients with systemic lupus erythematosus (SLE) compared with healthy subjects (control group). Moreover, we elucidated if clinical, therapeutic and biological parameters could affect the "in-vitro" intrinsic RS in patients with SLE. Intrinsic RS was determined by the quantification of the initial radiation-induced DNA double-strand breaks in peripheral lymphocytes, measured by pulsed-field gel electrophoresis from 52 patients with SLE and a control group consisting of 48 sex- and age-matched healthy subjects. No difference in intrinsic RS was found among both groups. However, SLE patients with anaemia, increased erythrocyte sedimentation rate and those with positive result for anti-La/SSB and anti-RNP antibodies showed significantly higher DNA double-strand breaks than those without them. In our study, patients with SLE did not have a higher intrinsic RS than healthy subjects. According to these results, and with the caution of being a limited laboratory study, the use of radiotherapy should not be avoided in patients with SLE when it is clinically needed.
Carrillo-Alascio
P L
PL
Unidad de Enfermedades Autoinmunes Sistémicas, Servicio de Medicina Interna, Hospital Universitario Virgen de las Nieves, Granada, Spain.
Sabio
J M
JM
Núñez-Torres
M I
MI
López
E
E
Muñoz-Gámez
J A
JA
Hidalgo-Tenorio
C
C
Jáimez
L
L
Martín
J
J
Jiménez-Alonso
J
J
D000328:Adult; D000888:Antibodies, Anti-Idiotypic / Q000097:blood; D001324:Autoantigens / Q000276:immunology; D016022:Case-Control Studies; D004247:DNA / Q000528:radiation effects*; D053903:DNA Breaks, Double-Stranded / Q000528:radiation effects; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D008180:Lupus Erythematosus, Systemic / Q000276:immunology / Q000473:pathology / Q000503:physiopathology*; D008214:Lymphocytes / Q000473:pathology / Q000528:radiation effects*; D008297:Male; D008875:Middle Aged; D011836:Radiation Tolerance / Q000502:physiology*; D011878:Radiotherapy / Q000009:adverse effects*; D012261:Ribonucleoproteins / Q000276:immunology; D012307:Risk Factors; D000097585:SS-B Antigen
D000888:Antibodies, Anti-Idiotypic; D001324:Autoantigens; D004247:DNA; D012261:Ribonucleoproteins; D000097585:SS-B Antigen