MxaF gene, a gene encoding alpha subunit of methanol dehydrogenase in and false growth of acetic acid bacteria on methanol.
MxaF gene, a gene encoding alpha subunit of methanol dehydrogenase, was investigated for acetic acid bacteria, and growth on methanol was examined for the bacteria by using various media. Of 21 strains of acetic acid bacteria studied, Acidomonas methanolica strains showed the presence of mxaF gene exclusively, and grew on a defined medium containing methanol. Further, none of the strains tested of which the growth on methanol had been previously reported, except for Acidomonas methanolica, showed the presence of mxaF gene or the growth on methanol. Precautions were taken against false growth on compounds used for identification of bacteria.
Laboratory of General and Applied Microbiology, Department of Applied Biology and Chemistry, Faculty of Applied Bioscience, Tokyo University of Agriculture, Tokyo, Japan.
Rei
R
Suzuki
Puspita
P
Lisdiyanti
Kazuo
K
Komagata
Tai
T
Uchimura
D019342:Acetic Acid / Q000378:metabolism; D016947:Acetobacteraceae / Q000145:classification / Q000201:enzymology* / Q000235:genetics* / Q000254:growth & development; D000429:Alcohol Oxidoreductases / Q000235:genetics* / Q000378:metabolism; D005798:Genes, Bacterial / Q000235:genetics*; D000432:Methanol / Q000378:metabolism*; D008969:Molecular Sequence Data; D012689:Sequence Homology, Nucleic Acid
D000429:Alcohol Oxidoreductases; C019641:alcohol dehydrogenase (acceptor); D019342:Acetic Acid; D000432:Methanol