Injection of an alpha-melanocyte stimulating hormone expression plasmid is effective in suppressing experimental autoimmune uveitis.
OBJECTIVE
The neuropeptide, alpha-melanocyte stimulating hormone (alpha-MSH), is an endogenous antagonist of inflammation. Injections of alpha-MSH peptide into inflamed tissues have been found to be very effective in suppressing autoimmune and endotoxin mediated diseases. We evaluated the potential to suppress ocular autoimmune disease (uveitis) by augmenting the expression of alpha-MSH through subconjunctival injections of naked adrenocorticotropic hormone amino acids 1-17 (ACTH1-17) plasmid.


METHODS
We clinically scored the uveitis over time in B10.RIII, C57BL/6, and melanocortin 5 receptor knock-out (MC5r((-/-))) mice with experimental autoimmune uveitis (EAU) that were conjunctively injected with a naked DNA plasmid encoding ACTH1-17 at the time of EAU onset and three days later. The post-EAU retina histology of plasmid injected eyes was examined, and post-EAU concentrations of alpha-MSH in aqueous humor was assayed by ELISA.


RESULTS
The subconjunctival injection of ACTH1-17 plasmid augmented the concentration of alpha-MSH in the aqueous humor of all post-EAU mice. The injection of ACTH1-17 suppressed the severity of EAU in the B10.RIII and C57BL/6 mice but the MC5r((-/-)) mice. In all the models of EAU, the ACTH1-17 injection helped to preserve the structural integrity of the retina; however, post-EAU aqueous humor was not immunosuppressive.


CONCLUSIONS
The subconjunctival injection of the alpha-MSH expression vector ACTH1-17 plasmid is effective in suppressing EAU. The suppressive activity is dependent on MC5r expression, and possibly works though alpha-MSH antagonism of inflammation than on alpha-MSH directly modulating immune cells. The results suggest that an effective therapy for uveitis could include a gene therapy approach based on delivering alpha-MSH.
Schepens Eye Research Institute, Department of Ophthalmology, Harvard Medical School, USA.
D J
DJ
Lee
D J
DJ
Biros
A W
AW
Taylor
D000324:Adrenocorticotropic Hormone / Q000235:genetics; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000008:administration & dosage; D001082:Aqueous Humor; D001327:Autoimmune Diseases / Q000235:genetics / Q000276:immunology* / Q000628:therapy; D005136:Eye Proteins / Q000276:immunology; D005620:Freund's Adjuvant; D015316:Genetic Therapy; D006801:Humans; D007165:Immunosuppression Therapy; D007267:Injections; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D023421:Models, Animal; D010446:Peptide Fragments / Q000235:genetics / Q000276:immunology; D010957:Plasmids; D015202:Protein Engineering; D044101:Receptors, Melanocortin / Q000172:deficiency; D012160:Retina / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D012177:Retinol-Binding Proteins / Q000276:immunology; D014605:Uveitis / Q000235:genetics / Q000276:immunology* / Q000628:therapy; D019444:Vaccines, DNA / Q000008:administration & dosage; D000521:alpha-MSH / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D000099214:Retinol-Binding Proteins, Interstitial
D000324:Adrenocorticotropic Hormone; D000893:Anti-Inflammatory Agents; D005136:Eye Proteins; D005620:Freund's Adjuvant; D010446:Peptide Fragments; D044101:Receptors, Melanocortin; D012177:Retinol-Binding Proteins; D019444:Vaccines, DNA; D000521:alpha-MSH; D000099214:Retinol-Binding Proteins, Interstitial; C086971:melanocortin 5 receptor; C028238:ACTH (1-17)