Homologue of mammalian apolipoprotein A-II in non-mammalian vertebrates.
Although apolipoprotein with molecular weight 14 kDa (apo-14 kDa) is associated with fish plasma high-density lipoproteins (HDLs), it remains to be determined whether apo-14 kDa is the homologue of mammalian apoA-II. We have obtained the full cDNA sequences that encode Japanese eel and rainbow trout apo-14 kDa. Homologues of Japanese eel apo-14 kDa sequence could be found in 14 fish species deposited in the DDBJ/EMBL/GenBank or TGI database. Fish apo-14 kDa lacks propeptide and contains more internal repeats than mammalian apoA-II. Nevertheless, phylogenetic analysis allowed fish apo-14 kDa to be the homologue of mammalian apoA-II. In addition, in silico cloning of the TGI, Ensembl, or NCBI database revealed apoA-IIs in dog, chicken, green anole lizard, and African clawed frog whose sequences had not so far been available, suggesting both apoA-I and apoA-II as fundamental constituents of vertebrate HDLs.
Science of Marine Resources, United Graduate School of Agricultural Science, Kagoshima University, Korimoto, Kagoshima, Japan.
Malay
M
Choudhury
Shoji
S
Yamada
Masaharu
M
Komatsu
Hideki
H
Kishimura
Seiichi
S
Ando
D000595:Amino Acid Sequence; D000818:Animals; D016633:Apolipoprotein A-II / Q000097:blood / Q000737:chemistry / Q000235:genetics*; D002645:Chickens; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D030561:Databases, Nucleic Acid; D004285:Dogs; D004524:Eels / Q000097:blood / Q000235:genetics*; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D008075:Lipoproteins, HDL / Q000097:blood; D008077:Lipoproteins, LDL / Q000097:blood; D008079:Lipoproteins, VLDL / Q000097:blood; D008099:Liver / Q000378:metabolism; D008116:Lizards; D008969:Molecular Sequence Data; D008970:Molecular Weight; D017686:Oncorhynchus mykiss / Q000235:genetics*; D010802:Phylogeny; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D014714:Vertebrates / Q000145:classification / Q000235:genetics*; D014982:Xenopus laevis
D016633:Apolipoprotein A-II; D018076:DNA, Complementary; D008075:Lipoproteins, HDL; D008077:Lipoproteins, LDL; D008079:Lipoproteins, VLDL