Studies of keratins in tongue coating samples of hepatitis B patients by mass spectrometry.
Pooled tongue coating samples from 64 hepatitis B patients and 24 healthy adults were studied and a major band of differential proteins was found by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). The differential proteins in this band were identified and proved to be keratins by liquid chromatography/tandem mass spectrometry (LC/MS/MS) and Western blot analysis. Furthermore, relative quantification of the identified keratins was performed via using stable isotopic labeling and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS), showing the higher expression level of these keratins in tongue coating samples of hepatitis B patients than healthy adults. These results provided additional information to understand the medical diagnosis depending on the tongue coating.
Fang
Fang
F
Shanghai Mass Spectrometry Center, Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences, 345 Lingling Road, Shanghai 200032, China.
Liu
Ping
P
Wang
Haoyang
H
Zhang
Li
L
Zhang
Jing
J
Gao
Yuping
Y
Zeng
Liming
L
Guo
Yinlong
Y
D000328:Adult; D005260:Female; D006509:Hepatitis B / Q000175:diagnosis* / Q000378:metabolism; D006801:Humans; D007633:Keratins / Q000737:chemistry* / Q000378:metabolism; D008297:Male; D008970:Molecular Weight; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D014059:Tongue / Q000737:chemistry*
D007633:Keratins