Inhibitory effect of glycoprotein isolated from Cudrania tricuspidata bureau on expression of inflammation-related cytokine in bisphenol A-treated HMC-1 cells.
Cudrania tricuspidata is one of the most omnipresent traditional herbal drugs for anti-inflammation and anti-tumor. The purpose of the present study was to determine whether the CTB glycoprotein regulates the inflammatory reaction stimulated by bisphenol A (BPA) in human mast cells (HMC-1). Thus, we investigated that CTB glycoprotein inhibits the degranulation of histamine, expression of extracellular signal-regulated kinase (ERK) and c-Jun N-terminal kinase (JNK), as a mitogen activated protein (MAP) kinase, nuclear transcription factors involving nuclear factor (NF)-kappaB and Activator protein (AP)-1, cyclooxygenase (COX)-2. The results indicated that CTB glycoprotein decreased gene expression of cytokines of IL-4, IFN-gamma, interleukin (IL)-1beta and cyclooxygenase (COX)-2 in BPA-stimulated HMC-1 cells. Hence, we speculate that CTB glycoprotein can use as a potent anti-inflammatory agent for inflammatory allergic diseases.
Shim
Jae-Uoong
JU
Molecular Biochemistry Laboratory, Biotechnology Research Institute, Chonnam National University, 300 Yongbong-Dong, Gwangju City, 500-757, South Korea.
Lim
Kye-Taek
KT
D000893:Anti-Inflammatory Agents / Q000494:pharmacology; D001559:Benzhydryl Compounds; D015550:Cell Degranulation; D002460:Cell Line; D016207:Cytokines / Q000096:biosynthesis*; D005786:Gene Expression Regulation / Q000187:drug effects; D006023:Glycoproteins / Q000494:pharmacology*; D006632:Histamine; D006801:Humans; D007249:Inflammation / Q000276:immunology; D008407:Mast Cells / Q000276:immunology; D029586:Moraceae / Q000737:chemistry*; D010636:Phenols / Q000494:pharmacology; D010940:Plant Proteins / Q000494:pharmacology; D000098826:Bisphenol A Compounds
D000893:Anti-Inflammatory Agents; D001559:Benzhydryl Compounds; D016207:Cytokines; D006023:Glycoproteins; D006632:Histamine; D010636:Phenols; D010940:Plant Proteins; C006780:bisphenol A; D000098826:Bisphenol A Compounds