[Expression of CD1a and CD207 in condyloma acuminatum epidermis].
OBJECTIVE
To investigate the expression of CD1a and CD207 in condyloma acuminatum (CA) epidermis and to understand its significance.


METHODS
The mRNA expression of CD1a and CD207 in six CA epidermal lesions and in six normal controls were detected using oligonucleotide microarrys and confirmed by semi-quantitative RT-PCR, and the protein level of CD1a and CD207 in six CA epidermal lesions and in six normal controls were measured by Western blot.


RESULTS
With microarrys, the mRNA expression of CD1a and CD207 were detected markedly down-regulated in six CA epidermal lesions as compared with that in six normal controls. Moreover, the down-regulation of CD1a and CD207 was verified by semi-quantitative RT-PCR. Western blot analysis showed that the protein expression of CD1a and CD207 in six CA epidermal lesions were significantly lower than that in normal controls.


CONCLUSIONS
The expression of CD1a and CD207 is markedly down-regulated in CA epidermis compared with that in normal epidermis, and the results may suggest that the number of LC in CA epidermis is decreased and the function is impaired.
Department of Dermatology, Shenzhen Municipal Children's Hospital, China. fjyfjy888@163.com
Jin-yun
JY
Feng
Guo-zhou
GZ
Rao
Yu-ping
YP
Liu
Ping
P
Li
Rong
R
Cui
Jian-hong
JH
Li
Jian-zhong
JZ
Liu
Zhen-hui
ZH
Peng
D000293:Adolescent; D000328:Adult; D015703:Antigens, CD / Q000235:genetics* / Q000378:metabolism; D018949:Antigens, CD1 / Q000235:genetics* / Q000378:metabolism; D015153:Blotting, Western; D003218:Condylomata Acuminata / Q000235:genetics*; D004817:Epidermis / Q000378:metabolism / Q000473:pathology; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D037181:Lectins, C-Type / Q000235:genetics* / Q000378:metabolism; D008297:Male; D037241:Mannose-Binding Lectins / Q000235:genetics* / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D055815:Young Adult
D015703:Antigens, CD; D018949:Antigens, CD1; C419393:CD1a antigen; C121916:CD207 protein, human; D037181:Lectins, C-Type; D037241:Mannose-Binding Lectins