Entire genome characterization of human papillomavirus type 16 from infected Thai women with different cytological findings.
Global prevalence of human papillomavirus type 16 (HPV16) exceeds that of other types. This project has been aimed at attaining basic molecular knowledge of HPV16 by sequencing the whole genome of HPV16 isolated from Thai women at various clinical stages of disease progression. Our group analyzed seven samples of HPV16 in infected women ranging from normal to cervical cancer and discovered two critical non-synonymous changes within the coding region converting the E2-219P prototype to E2-219T in cervical cancer and the L2-269S prototype to L2-269D in CIN III, respectively. Phylogenetic analysis based on the whole genome with special emphasis on the genes E2, E6, L1, and L2 showed the Thai samples to be more closely related to the European than the non-European strains. The vaccine strain's L1 polypeptides showed close relationship to our samples. The results provide basic data for future research on cervical cancer pathogenesis and representative data of HPV16 genome in Southeast Asia.
Center of Excellence in Clinical Virology, Department of Pediatrics, Chulalongkorn University, Bangkok 10330, Thailand.
Woradee
W
Lurchachaiwong
Pairoj
P
Junyangdikul
Sunchai
S
Payungporn
Jira
J
Chansaenroj
Pichet
P
Sampathanukul
Damrong
D
Tresukosol
Wichai
W
Termrungruanglert
Apiradee
A
Theamboonlers
Yong
Y
Poovorawan
D019943:Amino Acid Substitution; D036022:Capsid Proteins / Q000235:genetics; D016000:Cluster Analysis; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics*; D004268:DNA-Binding Proteins / Q000235:genetics; D005260:Female; D016679:Genome, Viral; D052162:Human papillomavirus 16 / Q000235:genetics* / Q000302:isolation & purification*; D006801:Humans; D008969:Molecular Sequence Data; D020125:Mutation, Missense; D009856:Oncogene Proteins, Viral / Q000235:genetics; D030361:Papillomavirus Infections / Q000821:virology*; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D013785:Thailand
D036022:Capsid Proteins; D004279:DNA, Viral; D004268:DNA-Binding Proteins; C054855:E2 protein, Human papillomavirus type 16; C063316:L2 protein, Human papillomavirus type 16; D009856:Oncogene Proteins, Viral