Novel fluorescent probe for analysis of hydroperoxides based on boron dipyrromethane fluorophore.
A new reagent, N-[2-(diphenylphosphino)ethyl]-4-(1,3,5,7-tetramethyl-4,4-difluoro-4-bora-3a,4a-diaza-s-indacene-8-yl)benzamide (DPPEA-BODIPY), was designed and synthesized for analysis of hydroperoxides. DPPEA-BODIPY fluoresces at low levels in the visible range (lambda(ex)/lambda(em) = 502 nm/515 nm) and reacts with hydroperoxides to produce DPPEA-BODIPY oxide, which fluoresces at high levels. The fluorescence intensity of the reaction mixture was observed to be linearly related to the methyl linoleate hydroperoxide (MeLOOH) concentration.
Research Center of Advanced Bionics, National Institute of Advanced Industrial Science and Technology (AIST), Katayanagi Institute, Tokyo University of Technology, Tokyo, Japan. suzuki-yoshio@aist.go.jp
Naoko
N
Inoue
Yoshio
Y
Suzuki
Kenji
K
Yokoyama
Isao
I
Karube
D001896:Boron Compounds / Q000138:chemical synthesis* / Q000737:chemistry; D004579:Electron Transport; D005456:Fluorescent Dyes / Q000138:chemical synthesis* / Q000737:chemistry; D006861:Hydrogen Peroxide / Q000032:analysis* / Q000737:chemistry; D008055:Lipids / Q000737:chemistry; D010720:Phosphines / Q000737:chemistry
D001896:Boron Compounds; D005456:Fluorescent Dyes; D008055:Lipids; C543109:N-(2-(diphenylphosphino)ethyl)-4-(1,3,5,7-tetramethyl-4,4-difluoro-4-bora-3a,4a-diaza-s-indacene-8-yl)benzamide; D010720:Phosphines; D006861:Hydrogen Peroxide; C044646:phosphine