The protective effect of Mucuna pruriens seeds against snake venom poisoning.
BACKGROUND
The seed, leaf and root of Mucuna pruriens have been used in traditional medicine for treatments of various diseases. In Nigeria, the seed is used as oral prophylactics for snakebite.


OBJECTIVE
To study the protective effects of Mucuna pruriens seed extract against the lethalities of various snake venoms.


METHODS
Rats were pre-treated with Mucuna pruriens seed extract and challenged with various snake venoms. The effectiveness of anti-Mucuna pruriens (anti-MPE) antibody to neutralize the lethalities of snake venoms was investigated by in vitro neutralization.


RESULTS
In rats, MPE pre-treatment conferred effective protection against lethality of Naja sputatrix venom and moderate protection against Calloselasma rhodostoma venom. Indirect ELISA and immunoblotting studies showed that there were extensive cross-reactions between anti-MPE IgG and venoms from many different genera of poisonous snakes, suggesting the involvement of immunological neutralization in the protective effect of MPE pre-treatment against snake venom poisoning. In vitro neutralization experiments showed that the anti-MPE antibodies effectively neutralized the lethalities of Asiatic cobra (Naja) venoms, but were not very effective against other venoms tested.


CONCLUSIONS
The anti-MPE antibodies could be used in the antiserum therapy of Asiatic cobra (Naja) bites.
Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia. tanngethong@yahoo.com.sg
Nget Hong
NH
Tan
Shin Yee
SY
Fung
Si Mui
SM
Sim
Enrico
E
Marinello
Roberto
R
Guerranti
John C
JC
Aguiyi
D000818:Animals; D000997:Antivenins / Q000302:isolation & purification / Q000494:pharmacology*; D004546:Elapid Venoms / Q000037:antagonists & inhibitors; D017815:Elapidae; D004797:Enzyme-Linked Immunosorbent Assay; D015151:Immunoblotting; D008297:Male; D016488:Medicine, African Traditional; D051379:Mice; D008813:Mice, Inbred ICR; D031299:Mucuna / Q000737:chemistry*; D009549:Nigeria; D010936:Plant Extracts / Q000494:pharmacology*; D011817:Rabbits; D051381:Rats; D017207:Rats, Sprague-Dawley; D012910:Snake Venoms / Q000037:antagonists & inhibitors*; D012911:Snakes
D000997:Antivenins; D004546:Elapid Venoms; D010936:Plant Extracts; D012910:Snake Venoms