New 19-oxygenated and 4-methylated steroids from the Formosan soft coral Nephthea chabroli.
Chemical investigation of the Formosan soft coral Nephthea chabroli resulted in the isolation of four new 19-oxygenated steroids, nebrosteroids I-L (1-4), together with a new 4alpha-methylated steroid, nebrosteroid M (5). The molecular structures of these isolated metabolites were elucidated on the basis of extensive spectroscopic analysis and by comparison of the data with those of related metabolites. Compounds 1-5 were evaluated for anti-inflammatory activity using RAW 264.7 macrophages.
Department of Marine Biotechnology and Resources, National Sun Yat-sen University, Kaohsiung, Taiwan.
Shi-Yie
SY
Cheng
Ya-Chiang
YC
Huang
Zhi-Hong
ZH
Wen
Chi-Hsin
CH
Hsu
Shang-Kwei
SK
Wang
Chang-Feng
CF
Dai
Chang-Yih
CY
Duh
D000818:Animals; D037421:Anthozoa / Q000737:chemistry*; D000893:Anti-Inflammatory Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D015153:Blotting, Western; D002460:Cell Line; D051546:Cyclooxygenase 2 / Q000378:metabolism; D008070:Lipopolysaccharides / Q000494:pharmacology; D008264:Macrophages / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D008745:Methylation; D051379:Mice; D008958:Models, Molecular; D015394:Molecular Structure; D052247:Nitric Oxide Synthase Type II / Q000378:metabolism; D010100:Oxygen / Q000737:chemistry; D013256:Steroids / Q000737:chemistry* / Q000302:isolation & purification* / Q000494:pharmacology; D013624:Taiwan
D000893:Anti-Inflammatory Agents; D008070:Lipopolysaccharides; D013256:Steroids; D052247:Nitric Oxide Synthase Type II; C077623:Ptgs2 protein, mouse; D051546:Cyclooxygenase 2; D010100:Oxygen