Study of microbiological and molecular typing aspects of paratuberculosis in sheep and goats in Northern Greece.
Mycobacterium avium subsp. paratuberculosis, an acid-fast bacterium is the agent of Johne's disease, an intestinal disease that causes poor nutrient intake in ruminants. During the period 1987-2003, 322 of 777 (41.4%) goat herds and 97 of 458 (21.1%) sheep flocks were found to be infected with M.a.paratuberculosis in Northern Greece. From goats, mycobacteria were isolated from 238 of 652 (36.5%) of intestinal tissues, 14 of 119 (11.8%) of lymph nodes and five of 369 (1.4%) of faecal samples. From sheep, mycobacteria were isolated from 25 of 162 (15.4%) of intestinal tissues, three of 41 (7.3%) of lymph nodes and two of 322 (0.6%) of faecal samples. Isolates were typed by pulsed-field gel electrophoresis (PFGE) and restriction fragment length polymorphism followed by hybridization to IS900 [IS900- restriction fragment-length polymorphism (RFLP)]. IS900-RFLP BstEII profiles C1 and C5 and PFGE profiles [2-19] and [29-15] were identified. These PFGE profiles have not been found outside Greece to date.
Dimareli-Malli
Z
Z
Veterinary Research Institute of Thessaloniki, NAGREF, Thessaloniki, Greece. dimareliz@hotmail.com
Stevenson
K
K
Sarris
K
K
Sossidou
K
K
D000818:Animals; D015373:Bacterial Typing Techniques / Q000662:veterinary; D016521:Electrophoresis, Gel, Pulsed-Field / Q000662:veterinary; D005243:Feces / Q000382:microbiology; D015511:Goat Diseases / Q000453:epidemiology / Q000382:microbiology*; D006041:Goats; D006115:Greece / Q000453:epidemiology; D016927:Mycobacterium avium subsp. paratuberculosis / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D010283:Paratuberculosis / Q000175:diagnosis / Q000453:epidemiology / Q000382:microbiology*; D016133:Polymerase Chain Reaction / Q000662:veterinary; D012150:Polymorphism, Restriction Fragment Length; D012756:Sheep; D012757:Sheep Diseases / Q000453:epidemiology / Q000382:microbiology*