Quantitation of biomolecules conjugated to nanoparticles by enzyme hydrolysis.
A novel method for quantifying biomolecules immobilised onto gold and silver nanoparticles is reported; fluorescent-labelled antibodies and DNA are hydrolysed on the surface of the nanoparticles by the addition of trypsin and DNase I, respectively, resulting in the release of the quantifiable fluorescent label into the bulk solution.
McKenzie
Fiona
F
Centre for Molecular Nanometrology, WestCHEM, Pure and Applied Chemistry, University of Strathclyde, Glasgow, UK.
Steven
Victoria
V
Ingram
Andrew
A
Graham
Duncan
D
D015374:Biosensing Techniques / Q000379:methods*; D002138:Calibration; D003432:Cross-Linking Reagents / Q000737:chemistry; D004247:DNA / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D003850:Deoxyribonuclease I / Q000378:metabolism; D005456:Fluorescent Dyes / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D006046:Gold / Q000737:chemistry; D006868:Hydrolysis; D016169:Lymphocyte Function-Associated Antigen-1 / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D053768:Metal Nanoparticles / Q000737:chemistry*; D012834:Silver / Q000737:chemistry; D014357:Trypsin / Q000378:metabolism
D003432:Cross-Linking Reagents; D005456:Fluorescent Dyes; D016169:Lymphocyte Function-Associated Antigen-1; D012834:Silver; D006046:Gold; D004247:DNA; D003850:Deoxyribonuclease I; D014357:Trypsin