[18F]SiFA-isothiocyanate: a new highly effective radioactive labeling agent for lysine-containing proteins.
A highly efficient (18)F-labeling synthon for universal protein labeling is reported. Diverse (18)F-labeled proteins of 66-144 kDa were prepared with [(18)F]SiFA-isothiocyanate synthesized by an isotopic (19)F for (18)F exchange at the silicon atom. Overall preparative radiochemical yields were 20-40 % after 40-50 min. No bone uptake of (18)F radioactivity was detected until 90 min post-injection of (18)F-SiFA-RSA; this demonstrates the metabolic stability of the [(18)F]SiFA moiety.
Montreal Neurological Institute, McGill University, Douglas Research Institute, Montreal, H3A 2B4, Canada.
Pedro
P
Rosa-Neto
Björn
B
Wängler
Ljuba
L
Iovkova
Guido
G
Boening
Andrew
A
Reader
Klaus
K
Jurkschat
Esther
E
Schirrmacher
D000818:Animals; D001059:Apoproteins / Q000737:chemistry; D002417:Cattle; D005459:Fluorides / Q000737:chemistry; D007074:Immunoglobulin G / Q000737:chemistry; D017879:Isothiocyanates / Q000737:chemistry* / Q000493:pharmacokinetics; D007553:Isotope Labeling; D008239:Lysine / Q000737:chemistry*; D008297:Male; D017646:Organosilicon Compounds / Q000737:chemistry; D011506:Proteins / Q000737:chemistry*; D019275:Radiopharmaceuticals / Q000737:chemistry* / Q000493:pharmacokinetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D012709:Serum Albumin / Q000737:chemistry; D014168:Transferrin / Q000737:chemistry
D001059:Apoproteins; D007074:Immunoglobulin G; D017879:Isothiocyanates; D017646:Organosilicon Compounds; D011506:Proteins; D019275:Radiopharmaceuticals; D012709:Serum Albumin; D014168:Transferrin; C028284:apotransferrin; D008239:Lysine; D005459:Fluorides