A naturally processed epitope on rotavirus VP7 glycoprotein recognized by HLA-A2.1-restricted cytotoxic CD8+ T cells.
Previous studies in mice have shown that CD8(+) T cells can mediate clearance of rotavirus (RV) infection and provide protection from reinfection. Moreover, it has been demonstrated that VP7 can induce a cross-reactive and vigorous specific cytotoxic T-lymphocyte (CTL) response. In this study, a panel of the potential human leukocyte antigen (HLA)-A2.1-restricted CTL epitopes derived from the human RV Wa strain VP7 glycoprotein were screened and assessed using reverse immunogenetics. The specific CTLs induced in vitro by p18-26 (LLNYILKSV)-pulsed autologous dendritic cells from the peripheral blood lymphocytes of healthy HLA-A2.1 donors released interferon-gamma (IFN-gamma) specifically upon stimulation of p18-26, and meanwhile these CTLs lysed p18-26-loaded T2 cells and human RV Wa strain-infected HLA-A2.1(+) Caco-2 cells in an HLA-A2.1-restricted manner. P18-26 was still proved to be immunogenic in vivo in HLA-A2.1/Kb transgenic mice. We propose that the newly identified CTL epitope restricted by HLA-A2.1 could aid in further study of RV-associated infection in humans.
Institute of Immunology, Third Military Medical University , District Shapingba, Chongqing, People's Republic of China. weijing06@yahoo.com.cn
Jing
J
Wei
Jintao
J
Li
Xiaoping
X
Zhang
Yan
Y
Tang
Jingxue
J
Wang
Yuzhang
Y
Wu
D000818:Animals; D017951:Antigen Presentation; D000956:Antigens, Viral / Q000276:immunology* / Q000378:metabolism; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D036022:Capsid Proteins / Q000276:immunology* / Q000378:metabolism; D002460:Cell Line; D000939:Epitopes / Q000276:immunology* / Q000378:metabolism; D015789:HLA-A2 Antigen / Q000276:immunology* / Q000378:metabolism; D006801:Humans; D007371:Interferon-gamma / Q000378:metabolism; D007963:Leukocytes, Mononuclear / Q000276:immunology / Q000378:metabolism; D008213:Lymphocyte Activation; D051379:Mice; D012401:Rotavirus / Q000276:immunology*
D000956:Antigens, Viral; D036022:Capsid Proteins; D000939:Epitopes; D015789:HLA-A2 Antigen; C050779:VP7 protein, Rotavirus; D007371:Interferon-gamma