Hybridization-sensitive fluorescent probe for long-term monitoring of intracellular RNA.
Nuclease-resistant hybridization probes for long-term intracellular RNA imaging were synthesized. Newly designed probes with a 2'-O-MeRNA structure showed high resistance against nucleases, which is in contrast to the low resistance of DNA probes. These modified probes retained the function on the on-off switching of fluorescence emission in sensitive response to RNA recognition, based on the mechanism of interdye excitonic interaction, and functioned effectively for the long-term monitoring of RNA-stained living cells.
Kubota
Takeshi
T
Advanced Science Institute, RIKEN (The Institute of Physical and Chemical Research), Wako, Saitama 351-1098, Japan.
Ikeda
Shuji
S
Yanagisawa
Hiroyuki
H
Yuki
Mizue
M
Okamoto
Akimitsu
A
D000818:Animals; D001483:Base Sequence; D002470:Cell Survival; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000737:chemistry / Q000378:metabolism*; D006367:HeLa Cells; D006801:Humans; D042541:Intracellular Space / Q000201:enzymology / Q000378:metabolism*; D009693:Nucleic Acid Hybridization; D009930:Organic Chemicals / Q000378:metabolism; D012313:RNA / Q000032:analysis* / Q000235:genetics; D015347:RNA Probes / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D020871:RNA Stability; D012260:Ribonucleases / Q000378:metabolism; D013997:Time Factors
D005456:Fluorescent Dyes; D009930:Organic Chemicals; D015347:RNA Probes; C057718:6-(Dimethylamino)-2-((1E,3E)-4-(4-(dimethylamino)phenyl)-1,3-butadien-1-yl)-1-ethylquinolinium perchlorate; D012313:RNA; D012260:Ribonucleases