Pharmacokinetics of oral calcitriol in healthy human based on the analysis with an enzyme immunoassay.
We investigated the pharmacokinetics of 2 microg calcitriol in eight healthy human after a single oral administration. Plasma calcitriol was analyzed by sensitive and quantitative enzyme immunoassay following intra- and inter-day validation. In subjects, AUC(0-infinity) and AUC(0-24h) were 267 and 246 pg h ml(-1), respectively. Although the mean plasma concentration of calcitriol in human subjects did not return to baseline at 24h post-dosing, the mean ratio of AUC(0-24h) to AUC(0-infinity) was greater than 80%. The C(max) of calcitriol was measured at 3.4h after administration as 50.0 pg ml(-1). From our results, it can offer new opportunities to design and determine individually appropriate therapeutic dosage regimens based on a pharmacokinetic profile and be used for bioequivalence and reevaluation study of generic drug.
Jin
Su-Eon
SE
Laboratory of Excellency for Drug and Gene Delivery, College of Pharmacy, Seoul National University, Republic of Korea.
Park
Jeong-Sook
JS
Kim
Chong-Kook
CK
D000284:Administration, Oral; D000328:Adult; D019540:Area Under Curve; D001794:Blood Pressure / Q000187:drug effects; D002117:Calcitriol / Q000008:administration & dosage / Q000009:adverse effects / Q000097:blood* / Q000493:pharmacokinetics*; D002118:Calcium / Q000097:blood; D004305:Dose-Response Relationship, Drug; D006339:Heart Rate / Q000187:drug effects; D006801:Humans; D007124:Immunoenzyme Techniques; D010710:Phosphates / Q000097:blood; D015203:Reproducibility of Results; D055815:Young Adult
D010710:Phosphates; D002117:Calcitriol; D002118:Calcium