Theoretical investigation of passive intestinal membrane permeability using Monte Carlo method to generate drug-like molecule population.
The purpose of the present study was to investigate the effect of the physiological and morphological differences between in vivo and in vitro systems on the estimation of in vivo effective intestinal membrane permeability (P(eff)) from in vitro permeability data (P(ivt,app)). Five hundred virtual drug-like compounds were generated using Monte Carlo method based on the distribution of octanol water partition coefficient, molecular weight, and pK(a). In vivo and in vitro membrane permeability was theoretically calculated from these parameters considering the transcellular, paracellular and unstirred water layer (UWL) permeation. More than 50% of drug-like compounds showed high P(eff) values. When the same pH value was used for in vivo and in vitro, the scattering of the P(ivt,app)-P(eff) plot was small, whereas it was large when different pH values were used. However, the extent of discrepancy depended on the physicochemical properties and permeation characteristics of a drug. When the pH effect was directly corrected on the P(eff) value, paracellular and UWL permeability was inappropriately corrected since the pH partition theory is only applicable for transcellular permeation. In vivo species differences of P(eff) and the fraction of a dose absorbed (Fa%) was also investigated for humans, rats and dogs. In conclusion, P(eff) estimation from in vitro data should be based on the theoretical method rather than simple linear regression.
Global Research & Development, Sandwich Laboratories, Research Formulation, Pfizer Inc., CT13 9NJ Sandwich, Kent, UK. Kiyohiko.Sugano@pfizer.com
Kiyohiko
K
Sugano
D000465:Algorithms; D000818:Animals; D002462:Cell Membrane / Q000378:metabolism*; D003198:Computer Simulation; D004058:Diffusion; D004285:Dogs; D006801:Humans; D006863:Hydrogen-Ion Concentration; D057927:Hydrophobic and Hydrophilic Interactions; D007408:Intestinal Absorption / Q000502:physiology*; D007413:Intestinal Mucosa / Q000378:metabolism*; D008954:Models, Biological; D008970:Molecular Weight; D009010:Monte Carlo Method; D010539:Permeability; D004364:Pharmaceutical Preparations / Q000737:chemistry / Q000378:metabolism*; D051381:Rats; D012995:Solubility
D004364:Pharmaceutical Preparations