7-O-methylkaempferol and -quercetin glycosides from the whole plant of Nervilia fordii.
Five new 7-O-methylkaempferol and -quercetin glycosides, namely, nervilifordins A-E (1-5), were isolated from the whole plant of Nervilia fordii, together with seven known flavonoids (6, 7, and 9-13) and one known coumarin (8). Their structures were elucidated on the basis of extensive spectroscopic analyses, including HSQC, HMBC, ROESY, and chemical methods. Compounds 1-3 and 6-13 were evaluated for their anti-herpes simplex virus 1 (HSV-1) activity and cytotoxicity on African green monkey kidney cells (Vero cells) in vitro. Of the tested compounds, only esculetin (8) exhibited antiviral activity against HSV-1, while the aglycones (11-13) showed stronger cytotoxicity on Vero cells than their glycosides (1-3, 6, and 7).
Tian
Li-Wen
LW
State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, People's Republic of China.
Pei
Ying
Y
Zhang
Ying-Jun
YJ
Wang
Yi-Fei
YF
Yang
Chong-Ren
CR
D000818:Animals; D000998:Antiviral Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D002522:Chlorocebus aethiops; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D006027:Glycosides / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D018259:Herpesvirus 1, Human / Q000187:drug effects*; D029595:Orchidaceae / Q000737:chemistry*; D010946:Plants, Medicinal / Q000737:chemistry*; D011794:Quercetin / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D014468:Umbelliferones / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D014709:Vero Cells
D000998:Antiviral Agents; D004365:Drugs, Chinese Herbal; D006027:Glycosides; D014468:Umbelliferones; D011794:Quercetin; C007628:esculetin