Staphylococcus aureus virulence expression is impaired by Lactococcus lactis in mixed cultures.
Staphylococcus aureus is responsible for numerous food poisonings due to the production of enterotoxins by strains contaminating foodstuffs, especially dairy products. Several parameters, including interaction with antagonistic flora such as Lactococcus lactis, a lactic acid bacterium widely used in the dairy industry, can modulate S. aureus proliferation and virulence expression. We developed a dedicated S. aureus microarray to investigate the effect of L. lactis on staphylococcal gene expression in mixed cultures. This microarray was used to establish the transcriptomic profile of S. aureus in mixed cultures with L. lactis in a chemically defined medium held at a constant pH (6.6). Under these conditions, L. lactis hardly affected S. aureus growth. The expression of most genes involved in the cellular machinery, carbohydrate and nitrogen metabolism, and stress responses was only slightly modulated: a short time lag in mixed compared to pure cultures was observed. Interestingly, the induction of several virulence factors and regulators, including the agr locus, sarA, and some enterotoxins, was strongly affected. This work clearly underlines the complexity of L. lactis antagonistic potential for S. aureus and yields promising leads for investigations into nonantibiotic biocontrol of this major pathogen.
Even
Sergine
S
INRA, UMR STLO, Rennes, France. sergine.even@rennes.inra.fr
Charlier
Cathy
C
Nouaille
Sébastien
S
Ben Zakour
Nouri L
NL
Cretenet
Marina
M
Cousin
Fabien J
FJ
Gautier
Michel
M
Cocaign-Bousquet
Muriel
M
Loubière
Pascal
P
Le Loir
Yves
Y
D000898:Antibiosis; D001426:Bacterial Proteins / Q000096:biosynthesis*; D018920:Coculture Techniques; D003470:Culture Media / Q000737:chemistry; D020869:Gene Expression Profiling; D013294:Lactococcus lactis / Q000502:physiology*; D020411:Oligonucleotide Array Sequence Analysis; D013211:Staphylococcus aureus / Q000502:physiology*; D037521:Virulence Factors / Q000096:biosynthesis*
D001426:Bacterial Proteins; D003470:Culture Media; D037521:Virulence Factors