Identification of a novel alkaliphilic esterase active at low temperatures by screening a metagenomic library from antarctic desert soil.
A novel esterase was identified through functional screening of a metagenomic library in Escherichia coli obtained from Antarctic desert soil. The 297-amino-acid sequence had only low (<29%) similarity to a putative esterase from Burkholderia xenovorans. The enzyme was active over a temperature range of 7 to 54 degrees C and at alkaline pH levels and is a potential candidate for industrial application.
Heath
Caroline
C
Department of Biotechnology, University of the Western Cape, Cape Town, South Africa.
Hu
Xiao Ping
XP
Cary
S Craig
SC
Cowan
Donald
D
D000864:Antarctic Regions; D019117:Burkholderia / Q000235:genetics; D004247:DNA / Q000235:genetics* / Q000302:isolation & purification*; D004795:Enzyme Stability; D004926:Escherichia coli / Q000235:genetics; D004950:Esterases / Q000235:genetics*; D015870:Gene Expression; D006863:Hydrogen-Ion Concentration; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D012988:Soil Microbiology; D013696:Temperature
D004247:DNA; D004950:Esterases