A heat-activated and thermoresistant telomerase activity in Leishmania major Friedlin.
Here we studied the telomerase activity of the human parasite Leishmania major. In this organism we have detected a high activity of this enzyme once several parameters such as heat activation, sequence of extension primer, and protein concentration are adjusted. The activity was not only heat activated, but also very resistant to heat denaturation. We believe L. major telomerase is an important activity and it may provide an adequate drug therapy target.
Centro de Biotecnología, Instituto de Estudios Avanzados, Ministerio del poder Popular para la Ciencia y Tecnología, Carretera Nacional Hoyo de la Puerta, Caracas, Venezuela.
Maria Mercedes
MM
Galindo
Evelyn
E
Rodriguez
Maria Gabriela
MG
Rojas
Katherine
K
Figarella
Riward
R
Campelo
Jose Luis
JL
Ramírez
D000818:Animals; D006358:Hot Temperature; D018320:Leishmania major / Q000201:enzymology* / Q000528:radiation effects*; D011489:Protein Denaturation / Q000528:radiation effects; D015800:Protozoan Proteins / Q000378:metabolism*; D019098:Telomerase / Q000378:metabolism*
D015800:Protozoan Proteins; D019098:Telomerase