Characterization of a Baculovirus newly isolated from the tea slug moth, Iragoidae fasciata.
The tea slug moth Iragoidae fasciata (Lepidoptera, Eucleidae) is one of the main insect pests that attack tea bushes. A new nucleopolyhedrovirus (NPV) called Iragoidae fasciata NPV (IrfaNPV) was recently isolated from diseased larvae. An 11,626 bp fragment of the viral genomic DNA containing the polyhedrin gene and other 12 genes was cloned and sequenced. Gene comparison and phylogenetic analysis showed that IrfaNPV is a member of the Group I NPVs. However, the genomic organization of IrfaNPV is highly distinct. In addition, electron microscopy analysis showed that IrfaNPV is a single nucleocapsid NPV (SNPV). An inoculation assay showed that IrfaNPV is semi-permissive in the Trichoplusia ni cell line Tn-5Bl-4. Bioassays on lethal concentration (LC(50)) and lethal time (LT(50)) were conducted to test the susceptibility of I. fasciata larvae to the virus.
Yang
Li-Rong
LR
Institute of Insect Sciences, Zhejiang University, Hangzhou, Zhejiang, 310029, PR China.
Qiang
Xiao
X
Zhang
Bao-Qin
BQ
Tang
Mei-Jun
MJ
Zhang
Chuan-Xi
CX
D000818:Animals; D028241:Camellia sinensis / Q000469:parasitology; D002460:Cell Line; D007814:Larva / Q000821:virology; D008969:Molecular Sequence Data; D009036:Moths / Q000821:virology*; D019251:Nucleocapsid / Q000235:genetics / Q000648:ultrastructure; D017924:Nucleopolyhedroviruses / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000648:ultrastructure; D010802:Phylogeny