Smoothing of, and parameter estimation from, noisy biophysical recordings.
Biophysically detailed models of single cells are difficult to fit to real data. Recent advances in imaging techniques allow simultaneous access to various intracellular variables, and these data can be used to significantly facilitate the modelling task. These data, however, are noisy, and current approaches to building biophysically detailed models are not designed to deal with this. We extend previous techniques to take the noisy nature of the measurements into account. Sequential Monte Carlo ("particle filtering") methods, in combination with a detailed biophysical description of a cell, are used for principled, model-based smoothing of noisy recording data. We also provide an alternative formulation of smoothing where the neural nonlinearities are estimated in a non-parametric manner. Biophysically important parameters of detailed models (such as channel densities, intercompartmental conductances, input resistances, and observation noise) are inferred automatically from noisy data via expectation-maximization. Overall, we find that model-based smoothing is a powerful, robust technique for smoothing of noisy biophysical data and for inference of biophysical parameters in the face of recording noise.
Huys
Quentin J M
QJ
Gatsby Computational Neuroscience Unit, University College London, London, United Kingdom. qhuys@cantab.net
Paninski
Liam
L
D000465:Algorithms; D001185:Artificial Intelligence; D055592:Biophysical Phenomena; D002468:Cell Physiological Phenomena; D019295:Computational Biology; D003198:Computer Simulation; D055724:Electrophysiological Phenomena; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D008954:Models, Biological; D009010:Monte Carlo Method; D018709:Statistics, Nonparametric