Engineered two-helix small proteins for molecular recognition.
Less is more: By starting with a high-affinity HER2-binding 3-helix affibody molecule, we successfully developed 2-helix small protein binders with 5 nM affinities by using a combination of several different strategies. Our efforts clearly suggest that 2-helix small proteins against important tumor targets can be obtained by rational protein design and engineering.
Webster
Jack M
JM
Biosciences, Global Research, General Electric Company, 1 Research Circle, Niskayuna, NY 12309, USA.
Zhang
Rong
R
Gambhir
Sanjiv S
SS
Cheng
Zhen
Z
Syud
Faisal A
FA
D000595:Amino Acid Sequence; D004220:Disulfides / Q000737:chemistry; D008969:Molecular Sequence Data; D050505:Mutant Proteins / Q000737:chemistry; D011485:Protein Binding; D015202:Protein Engineering / Q000379:methods*; D017433:Protein Structure, Secondary; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000737:chemistry*; D017386:Sequence Homology, Amino Acid
D004220:Disulfides; D050505:Mutant Proteins; D018719:Erb-b2 Receptor Tyrosine Kinases