Synthesis and base-pairing properties of C-nucleotides having 1-substituted 1H-1,2,3-triazoles.
Oligonucleotides including C-nucleotides having 1-substitued 1H-1,2,3-triazoles as artificial nucleobases were conveniently synthesized by the post-elongation modification method using the copper(I)-catalyzed alkyne-azide 1,3-dipolar cycloaddition (CuAAC) reaction. The base-pairing properties of the triazole nucleobase analogs in forming duplexes with oligonucleotides were investigated by the T(m) experiments.
Graduate School of Pharmaceutical Sciences, Osaka University, 1-6 Yamadaoka, Suita, Osaka 565-0871, Japan.
Motoi
M
Nakahara
Takeshi
T
Kuboyama
Akihiro
A
Izawa
Yoshiyuki
Y
Hari
Takeshi
T
Imanishi
Satoshi
S
Obika
D020029:Base Pairing; D008722:Methods; D009691:Nucleic Acid Denaturation; D009693:Nucleic Acid Hybridization; D009711:Nucleotides / Q000138:chemical synthesis* / Q000737:chemistry; D009841:Oligonucleotides; D044366:Transition Temperature; D014230:Triazoles / Q000737:chemistry*
D009711:Nucleotides; D009841:Oligonucleotides; D014230:Triazoles