The differentiation-inducing effect of conditioned media obtained from dental pulp cells.
OBJECTIVE
Conditioned media (CM) from human dental pulp cells (HDPC) was investigated for its effects on the proliferation and differentiation of HDPC and MG63 cells.


METHODS
CM prepared from the primary culture of HDPC was used for the culture of HDPC and MG63. Cell growth, alkaline phosphatase (ALPase) activity, mRNA expression of differentiation-related genes, and mineralization of the HDPC and MG63 cells in the media containing CM were assessed.


RESULTS
HDPC CM increased the cell growth of HDPC but decreased MG63 cell growth. ALPase activity and the mineralization of both HDPC and MG63 were enhanced by HDPC CM. The CM also up-regulated the expressions of DSPP, DMP-1, and OCN mRNA in HDPC. Pretreatment of HDPC CM with a neutralizing antibody against TGF-beta completely eliminated the effect of CM on ALPase activity in HDPC.


CONCLUSIONS
HDPC was able to secrete odontogenic differentiation-inducing factors, in which TGF-beta seems to a key element of the CM effects.
Kim
Na Ryoung
NR
Department of Dental Biomaterials Science, School of Dentistry, Seoul National University, Seoul, Korea.
Lee
Dong Hee
DH
Ahn
Sug-Joon
SJ
Lee
In-Seop
IS
Yang
Hyeong-Cheol
HC
D000293:Adolescent; D000469:Alkaline Phosphatase / Q000096:biosynthesis; D002113:Calcification, Physiologic / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D002648:Child; D017077:Culture Media, Conditioned / Q000494:pharmacology*; D003782:Dental Pulp / Q000166:cytology / Q000502:physiology*; D003809:Dentin, Secondary / Q000254:growth & development / Q000378:metabolism*; D016326:Extracellular Matrix Proteins / Q000096:biosynthesis; D018507:Gene Expression Regulation, Developmental; D006801:Humans; D009804:Odontoblasts / Q000378:metabolism*; D015675:Osteocalcin / Q000096:biosynthesis; D010750:Phosphoproteins / Q000096:biosynthesis; D012333:RNA, Messenger / Q000096:biosynthesis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012795:Sialoglycoproteins; D016212:Transforming Growth Factor beta / Q000037:antagonists & inhibitors / Q000502:physiology*; D015854:Up-Regulation; D000099311:Dentin Sialophosphoprotein
D000469:Alkaline Phosphatase; D017077:Culture Media, Conditioned; D016326:Extracellular Matrix Proteins; D015675:Osteocalcin; D010750:Phosphoproteins; D012333:RNA, Messenger; D012795:Sialoglycoproteins; D016212:Transforming Growth Factor beta; D000099311:Dentin Sialophosphoprotein; C513309:DMP1 protein, human