A silver staining procedure for nucleic acids in polyacrylamide gels without fixation and pretreatment.
Silver staining of nucleic acid has been widely used in molecular marker analysis such as simple sequence repeat (SSR), single-strand conformation polymorphism (SSCP), and amplified fragment-length polymorphism (AFLP). Many alternatives to silver staining methods have been described, but these methods are not efficient or cost-effective. Here we report a silver staining method that requires less than 10min for one gel and can save chemicals as well. It has a detection limit of approximately 5.6pg of DNA/mm(2) in nondenaturing polyacrylamide gels and 12.8pg/mm(2) in denaturing polyacrylamide gels.
An
Z W
ZW
Rubber Research Institute, Chinese Academy of Tropical Agricultural Sciences, Danzhou, Hainan 571737, China.
Xie
L L
LL
Cheng
H
H
Zhou
Y
Y
Zhang
Q
Q
He
X G
XG
Huang
H S
HS
D000180:Acrylic Resins; D054458:Amplified Fragment Length Polymorphism Analysis; D004247:DNA / Q000032:analysis*; D004591:Electrophoresis, Polyacrylamide Gel; D005404:Fixatives / Q000737:chemistry*; D018807:Polymorphism, Single-Stranded Conformational; D016622:Silver Staining / Q000379:methods*
D000180:Acrylic Resins; D005404:Fixatives; C016680:polyacrylamide gels; D004247:DNA