The identification of proteoglycan, collagen and neuron in precursor cells from human fetal spinal cord.
After spinal cord injury (SCI), a loss of myelinating oligodendrocytes and neurons occurs. The functional recovery of injured spinal cords is the principal objective of SCI repair. Cell transplantation may prove beneficial to help replace lost myelin and spinal cord circuitry. In this study, we demonstrated that neural precursor cells (hNPCs) from human fetal spinal cord express three types of proteoglycan proteins-chondroitin sulfate, keratan sulfate, and cartilage proteoglycan (an extracellular matrix detected in normal spinal cord), and non-proteoglycan matrix collagen. Both proteoglycan and collagen evidenced profound immunoreactivity in double-stained cell clusters. In addition, whether or not hNPCs were capable of differentiating into a variety of cells, including GABAergic and cholinergic neurons, were assessed. The differentiated cells of eight passages grown on a monolayer expressed the human nuclear protein (HNu), the progenitor marker nestin, GAD, ChAT, TJU, and MAP-2. These results indicate that hNPCs may prove to be candidate cells for therapeutic SCI strategies.
Department of Neurosurgery, Gachon Medical College, Gil Medical Center, Incheon, South Korea.
Chan-Jong
CJ
Yoo
Young-Mi
YM
Yoo
Yong-Jung
YJ
Kim
D000109:Acetylcholine / Q000378:metabolism; D002478:Cells, Cultured; D002574:Cervical Vertebrae / Q000196:embryology; D002795:Choline O-Acetyltransferase / Q000378:metabolism; D002809:Chondroitin Sulfates / Q000378:metabolism; D003094:Collagen / Q000378:metabolism*; D005968:Glutamate Decarboxylase / Q000378:metabolism; D006801:Humans; D007381:Intermediate Filament Proteins / Q000378:metabolism; D007632:Keratan Sulfate / Q000378:metabolism; D008869:Microtubule-Associated Proteins / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000378:metabolism; D064231:Nestin; D055495:Neurogenesis; D009474:Neurons / Q000166:cytology / Q000378:metabolism*; D009687:Nuclear Proteins / Q000378:metabolism; D011509:Proteoglycans / Q000378:metabolism*; D013116:Spinal Cord / Q000166:cytology / Q000196:embryology* / Q000378:metabolism; D013234:Stem Cells / Q000378:metabolism*; D013904:Thoracic Vertebrae / Q000196:embryology; D014404:Tubulin / Q000378:metabolism; D005680:gamma-Aminobutyric Acid / Q000378:metabolism
D007381:Intermediate Filament Proteins; C489788:MAP2 protein, human; D008869:Microtubule-Associated Proteins; C577484:NES protein, human; D009419:Nerve Tissue Proteins; D064231:Nestin; D009687:Nuclear Proteins; D011509:Proteoglycans; D014404:Tubulin; D005680:gamma-Aminobutyric Acid; D002809:Chondroitin Sulfates; D003094:Collagen; D007632:Keratan Sulfate; D002795:Choline O-Acetyltransferase; D005968:Glutamate Decarboxylase; D000109:Acetylcholine