Thermal design and turbidity sensor for autonomous bacterial growth measurements in spaceflight.
For application of biological air filters in manned spacecraft, research on bacterial growth is carried out under microgravity conditions. For the BIOFILTER experiment, flown in 2005 on FOTON M2, eight turbidity sensors to measure the growth rate of the bacterium Xanthobacter autotrophicus GJ10 were used. Also thermal management provisions were implemented to control the internal temperature. The design and performance of the BIOFILTER equipment as well as results of the biological ground reference experiments performed in 2006 are discussed. High-performance thermal (vacuum) insulation (lambda= 0.7 mW/mK) and phase change material were implemented, keeping the BIOFILTER internal temperature below 16 degrees C during the 4-day integration period between transport and launch. After launch, in microgravity, the growth of X. autotrophicus GJ10 was successfully triggered by a temperature increase by using an internal heater to 26 degrees C. Although the operation of the sensor electronics was not fully satisfying, the bacterial growth was measured with the sensors, revealing growth rates between 0.046 and 0.077 h(-1) in microgravity, that is, approximately 1.5-2.5 times slower than routinely measured on Earth under optimal laboratory conditions. For the ground-reference experiments the equipment box, containing the eight sensors, was placed on a random positioning machine performing random rotations at 0.5 degrees /min (settling compensation) and 90 degrees /min (microgravity simulation) while the environment was controlled, accurately repeating the BIOFILTER internal temperature profile. Despite the rotation speed differences, growth rates of 0.115 h(-1) were confirmed by both the ground reference experiments. Biological interpretation of the measurements is, however, compromised owing to poor mixing and other unknown physical and biological phenomena that need to be addressed for further space experiments using these kinds of systems.
van Benthem
Roel
R
National Aerospace Laboratory NLR, Emmeloord, The Netherlands. benthem@nlr.nl
Krooneman
Janneke
J
de Grave
Wubbo
W
Hammenga-Dorenbos
Hilma
H
D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods*; D002138:Calibration; D005374:Filtration; D050296:Microbial Viability; D009391:Nephelometry and Turbidimetry / Q000295:instrumentation* / Q000379:methods*; D013026:Space Flight; D013696:Temperature; D020584:Xanthobacter