Immunostimulatory effects of natural human interferon-alpha (huIFN-alpha) on carps Cyprinus carpio L.
Human interferon-alpha (huIFN-alpha) is an important immunomodulatory substance used in the treatment and prevention of numerous infectious and immune-related diseases in animals. However, the immunostimulatory effects of huIFN-alpha in fish remain to be investigated. In the current study, the immune responses of the carp species Cyprinus carpio L. to treatment with huIFN-alpha were analyzed via measurement of superoxide anion production, phagocytic activity and the expression of cytokine genes including interleukin-1beta, tumor necrosis factor-alpha and interleukin 10. Low doses of huIFN-alpha were administered orally once a day for 3 days, and sampling was carried out at 1, 3 and 5 days post-treatment. Our results indicate that a low dose of huIFN-alpha significantly increased phagocytic activity and superoxide anion production in the carp kidney. The huIFN-alpha-treated fish also displayed a significant upregulation in cytokine gene expression. The current study demonstrates the stimulatory effects of huIFN-alpha on the carp immune system and highlights the immunomodulatory role of huIFN-alpha in fish.
Watanuki
Hironobu
H
Faculty of Agriculture, University of Miyazaki, Miyazaki, Japan.
Chakraborty
Gunimala
G
Korenaga
Hiroki
H
Kono
Tomoya
T
Shivappa
R B
RB
Sakai
Masahiro
M
D000276:Adjuvants, Immunologic / Q000008:administration & dosage / Q000494:pharmacology*; D000284:Administration, Oral; D000818:Animals; D001483:Base Sequence; D002347:Carps / Q000235:genetics / Q000276:immunology* / Q000502:physiology; D016207:Cytokines / Q000235:genetics; D017931:DNA Primers / Q000235:genetics; D015870:Gene Expression / Q000187:drug effects; D006801:Humans; D016898:Interferon-alpha / Q000008:administration & dosage / Q000494:pharmacology*; D016753:Interleukin-10 / Q000235:genetics; D053583:Interleukin-1beta / Q000235:genetics; D007668:Kidney / Q000187:drug effects / Q000276:immunology / Q000502:physiology; D010586:Phagocytes / Q000187:drug effects / Q000276:immunology / Q000502:physiology; D010587:Phagocytosis; D013481:Superoxides / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics
D000276:Adjuvants, Immunologic; D016207:Cytokines; D017931:DNA Primers; D016898:Interferon-alpha; D053583:Interleukin-1beta; D014409:Tumor Necrosis Factor-alpha; D013481:Superoxides; D016753:Interleukin-10