Spectroscopic characterization of DMPC/DOTAP cationic liposomes and their interactions with DNA and drugs.
Gene and synthetic drug-delivery vectors have been developed and characterized to treat several genetic diseases and cancers. Our study aims at characterizing cationic liposomes containing the zwitterionic phospholipid DMPC and the cationic lipid DOTAP as well as their interactions with two types of DNA and a new class of antineoplastic agents derived from arylchloroethylureas (CEU). Results obtained using FTIR spectroscopy as well as (31)P and (2)H NMR indicate that DMPC and DOTAP form cationic liposomes in a highly disordered fluid phase at a molar ratio of 1:1. In addition, the FTIR results indicate that the presence of DNA or CEUs within the liposomes does not significantly affect the conformational order of both the DMPC and DOTAP acyl chains. Our results therefore provide a detailed characterization of complexes between cationic liposomes and both DNA and drugs and indicate that these complexes are stable and fluid assemblies.
Labbé
Jean-François
JF
Département de Chimie, Centre de Recherche sur la Fonction, Structure et Ingénierie des Protéines, Centre de Recherche sur les Matériaux Avancés, Université Laval, Québec, Canada.
Cronier
Francis
F
C-Gaudreault
René
R
Auger
Michèle
M
D000818:Animals; D000970:Antineoplastic Agents / Q000737:chemistry / Q000378:metabolism; D002412:Cations / Q000737:chemistry; D002417:Cattle; D004247:DNA / Q000737:chemistry / Q000378:metabolism*; D004134:Dimyristoylphosphatidylcholine / Q000737:chemistry* / Q000378:metabolism; D004337:Drug Carriers / Q000737:chemistry / Q000378:metabolism; D005229:Fatty Acids, Monounsaturated / Q000737:chemistry* / Q000378:metabolism; D008081:Liposomes / Q000737:chemistry* / Q000378:metabolism*; D019906:Nuclear Magnetic Resonance, Biomolecular; D010671:Phenylurea Compounds / Q000737:chemistry / Q000378:metabolism*; D010957:Plasmids; D000644:Quaternary Ammonium Compounds / Q000737:chemistry* / Q000378:metabolism; D017550:Spectroscopy, Fourier Transform Infrared; D013696:Temperature; D013950:Thymus Gland / Q000737:chemistry
D000970:Antineoplastic Agents; D002412:Cations; D004337:Drug Carriers; D005229:Fatty Acids, Monounsaturated; D008081:Liposomes; D010671:Phenylurea Compounds; D000644:Quaternary Ammonium Compounds; D004247:DNA; C070046:1,2-dioleoyloxy-3-(trimethylammonium)propane; D004134:Dimyristoylphosphatidylcholine