In-vitro radiosensitivity in patients with systemic lupus erythematosus.
To determine the "in-vitro" intrinsic cell radiosensitivity (RS) as a risk indicator of radiation-related side-effects in patients with systemic lupus erythematosus (SLE) compared with healthy subjects (control group). Moreover, we elucidated if clinical, therapeutic and biological parameters could affect the "in-vitro" intrinsic RS in patients with SLE. Intrinsic RS was determined by the quantification of the initial radiation-induced DNA double-strand breaks in peripheral lymphocytes, measured by pulsed-field gel electrophoresis from 52 patients with SLE and a control group consisting of 48 sex- and age-matched healthy subjects. No difference in intrinsic RS was found among both groups. However, SLE patients with anaemia, increased erythrocyte sedimentation rate and those with positive result for anti-La/SSB and anti-RNP antibodies showed significantly higher DNA double-strand breaks than those without them. In our study, patients with SLE did not have a higher intrinsic RS than healthy subjects. According to these results, and with the caution of being a limited laboratory study, the use of radiotherapy should not be avoided in patients with SLE when it is clinically needed.
Unidad de Enfermedades Autoinmunes Sistémicas, Servicio de Medicina Interna, Hospital Universitario Virgen de las Nieves, Granada, Spain.
P L
PL
Carrillo-Alascio
J M
JM
Sabio
M I
MI
Núñez-Torres
E
E
López
J A
JA
Muñoz-Gámez
C
C
Hidalgo-Tenorio
L
L
Jáimez
J
J
Martín
J
J
Jiménez-Alonso
D000328:Adult; D000888:Antibodies, Anti-Idiotypic / Q000097:blood; D001324:Autoantigens / Q000276:immunology; D016022:Case-Control Studies; D004247:DNA / Q000528:radiation effects*; D053903:DNA Breaks, Double-Stranded / Q000528:radiation effects; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D008180:Lupus Erythematosus, Systemic / Q000276:immunology / Q000473:pathology / Q000503:physiopathology*; D008214:Lymphocytes / Q000473:pathology / Q000528:radiation effects*; D008297:Male; D008875:Middle Aged; D011836:Radiation Tolerance / Q000502:physiology*; D011878:Radiotherapy / Q000009:adverse effects*; D012261:Ribonucleoproteins / Q000276:immunology; D012307:Risk Factors; D000097585:SS-B Antigen
D000888:Antibodies, Anti-Idiotypic; D001324:Autoantigens; D004247:DNA; D012261:Ribonucleoproteins; D000097585:SS-B Antigen