Decreased level of cyclin A2 in rat cochlea development and cochlear stem cell differentiation.
Cyclin A2 plays a major role in cell cycle modulation. Cyclin A2 level has not been determined in the inner ear yet. RT-PCR, Western blotting, immunohistochemistry and immunocytochemistry were used to measure cyclin A2 expression in rat cochlea tissues of different ages, isolated cochlear stem cells and stem cell-derived differentiated cells. The results indicated that cyclin A2 level in cochlea tissues decreased gradually from newborn to adult. Furthermore, cyclin A2 level fell down after differentiation of cochlear stem cells. It was suggested that cyclin A2 might be involved in the modulation of rat cochlea development and cochlear stem cell differentiation.
Chen
Jun
J
Department of Otolaryngology, Xijing Hospital, The Fourth Military Medical University, 17 Changle Western Road, Xi'an, Shaanxi Province 710032, China.
Wang
Feng
F
Gao
Xue
X
Zha
Dingjun
D
Xue
Tao
T
Cheng
Xin
X
Zhong
Cuiping
C
Han
Yu
Y
Qiu
Jianhua
J
D000818:Animals; D000831:Animals, Newborn; D002454:Cell Differentiation; D003051:Cochlea / Q000166:cytology / Q000254:growth & development / Q000378:metabolism*; D019925:Cyclin A / Q000378:metabolism*; D056751:Cyclin A2; D007150:Immunohistochemistry; D051381:Rats; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013234:Stem Cells / Q000502:physiology*
C503722:Ccna2 protein, rat; D019925:Cyclin A; D056751:Cyclin A2