Immunohistochemical localization of Krüppel-like factor 6 in the mouse forebrain.
Krüppel-like factor 6 (KLF6) is a transcriptional regulator that shows widespread distribution in the peripheral organs of the body. However, it remains uncertain where KLF6 is expressed in the adult forebrain under physiological conditions. Therefore, the present study investigated the spatial patterns of KLF6 expression and identified cell types expressing KLF6 in the forebrain. KLF6 immunoreactivity was widely seen throughout the forebrain including the olfactory bulb, cerebral cortex, hippocampus, septum, amygdala, basal ganglia, thalamus, and hypothalamus. Moreover, KLF6-positive cells were also detected in the radial migratory stream (RMS) and subventricular zone. Immunofluorescent double-labeling revealed that KLF6-immunoreactive cells were co-localized with neuronal nuclei or platelet endothelial cell adhesion molecule-1, a mature neuronal and endothelial marker, respectively, in most forebrain regions. In the RMS, KLF6 was co-expressed with polysialic neural cell adhesion molecule, a marker of neuronal progenitor cells. This is the first report showing that KLF6 protein is expressed in various regions of the adult forebrain and KLF6-positive cells manifest neuronal or endothelial phenotypes under physiological conditions.
Jeong
Kyoung Hoon
KH
Department of Pharmacology, Cell Death Disease Research Center, College of Medicine, The Catholic University of Korea, 505 Banpo-dong, Socho-gu, 137-701 Seoul, Republic of Korea.
Kim
Seul-Ki
SK
Kim
Seong Yun
SY
Cho
Kyung-Ok
KO
D000818:Animals; D002467:Cell Nucleus / Q000737:chemistry; D004268:DNA-Binding Proteins; D015870:Gene Expression; D007150:Immunohistochemistry; D000075723:Kruppel-Like Factor 6; D051741:Kruppel-Like Transcription Factors / Q000032:analysis*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009419:Nerve Tissue Proteins / Q000032:analysis; D039842:Neural Cell Adhesion Molecule L1 / Q000032:analysis; D019006:Neural Cell Adhesion Molecules / Q000032:analysis; D009474:Neurons / Q000737:chemistry / Q000648:ultrastructure; D009687:Nuclear Proteins / Q000032:analysis; D019408:Platelet Endothelial Cell Adhesion Molecule-1 / Q000032:analysis; D016548:Prosencephalon / Q000737:chemistry*; D011518:Proto-Oncogene Proteins / Q000032:analysis*; D012794:Sialic Acids / Q000032:analysis
D004268:DNA-Binding Proteins; C491358:Klf6 protein, mouse; D000075723:Kruppel-Like Factor 6; D051741:Kruppel-Like Transcription Factors; D009419:Nerve Tissue Proteins; C492380:NeuN protein, mouse; D039842:Neural Cell Adhesion Molecule L1; D019006:Neural Cell Adhesion Molecules; D009687:Nuclear Proteins; D019408:Platelet Endothelial Cell Adhesion Molecule-1; D011518:Proto-Oncogene Proteins; D012794:Sialic Acids; C103469:polysialyl neural cell adhesion molecule