Anti-angiogenic activity of julibroside J8, a natural product isolated from Albizia julibrissin.
OBJECTIVE
The purpose of this study was to investigate the anti-angiogenic properties of julibroside J(8), a triterpenoid saponin isolated from Albizia julibrissin.


METHODS
In the presence of juliborside J(8,) the growth of human microvascular endothelial cells (HMEC-1), four human tumor cell lines, and a normal cell line (MRC-5) was evaluated by MTT assay. The in vivo anti-angiogenic effect of julibroside J(8) was evaluated on a chorioallantoic membrane (CAM) and in transplanted colon carcinoma cells in a nude mice neovascularisation model.


RESULTS
Treatment with 0.5-4 microg/ml julibroside J(8) resulted in dose-dependent inhibition of growth, migration, and tube formation in HMEC-1 cells; julibroside J(8) also inhibited the formation of microvessels on CAM at a concentration of 10-50 microg/egg and reduced vessel density within tumor at a concentration of 0.5-3mg/kg.


CONCLUSIONS
Julibroside J(8) may be a potent anti-angiogenetic and cytotoxic drug; further investigation is warranted.
Laboratory of Natural Medicine, School of Medicine and Pharmaceutics, Jiangnan University, Wuxi, Jiangsu, PR China.
Hui
H
Hua
Lei
L
Feng
Xiao-Ping
XP
Zhang
Lian-Fen
LF
Zhang
Jian
J
Jin
D031289:Albizzia / Q000737:chemistry*; D020533:Angiogenesis Inhibitors / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use; D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use; D002460:Cell Line; D045744:Cell Line, Tumor; D002642:Chick Embryo; D049033:Chorioallantoic Membrane; D003110:Colonic Neoplasms; D004305:Dose-Response Relationship, Drug; D042783:Endothelial Cells; D006801:Humans; D051379:Mice; D055806:Microvessels / Q000187:drug effects; D008517:Phytotherapy; D024301:Plant Bark; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology* / Q000627:therapeutic use; D018547:Plant Stems; D010946:Plants, Medicinal / Q000737:chemistry; D011134:Polysaccharides / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology* / Q000627:therapeutic use; D012503:Saponins / Q000494:pharmacology* / Q000627:therapeutic use
D020533:Angiogenesis Inhibitors; D000972:Antineoplastic Agents, Phytogenic; C513093:Julibroside J8; D010936:Plant Extracts; D011134:Polysaccharides; D012503:Saponins