Heterotrophic culture of Chlorella protothecoides in various nitrogen sources for lipid production.
The influences of urea, yeast extract, and nitrate as the nitrogen source on heterotrophic growth of four strains of Chlorella protothecoides were investigated in 9-day feed-batch cultures. Biomass dry weight concentration (DWC) and lipid yield (LY) of the four strains in all media were compared. The highest LY in 9 days was 654 mg/L/day by UTEX 255 in 2.4 g/L KNO(3) medium with a biomass DWC of 11.7 g/L and lipid content of 50.5%. Using green autotrophic seeds instead of yellow heterotrophic seeds improved the biomass DWC (13.1 vs. 11.7 g/L), LY (850 vs. 654 mg/L/day), and lipid to glucose consumption ratio (0.607 vs. 0.162). Moreover, 17.0 g/L DWC and 489 mg/L/day LY were obtained from the sequentially mixed-nitrogen medium, and the lipid to glucose consumption ratio was improved to 0.197 from 0.162 in 2.4 g/L nitrate medium and from 0.108 in 4.2 g/L yeast extract medium in the first batch.
Shen
Y
Y
Department of Biological and Agricultural Engineering, Kansas State University, Manhattan, KS, 66506, USA.
Yuan
W
W
Pei
Z
Z
Mao
E
E
D018533:Biomass; D018929:Cell Culture Techniques / Q000379:methods*; D002708:Chlorella / Q000187:drug effects* / Q000254:growth & development*; D003470:Culture Media / Q000494:pharmacology; D000431:Ethanol / Q000378:metabolism; D005285:Fermentation / Q000187:drug effects; D005947:Glucose / Q000378:metabolism; D052836:Heterotrophic Processes / Q000187:drug effects*; D008055:Lipids / Q000096:biosynthesis*; D009584:Nitrogen / Q000494:pharmacology*; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000378:metabolism
D003470:Culture Media; D008055:Lipids; D000431:Ethanol; D005947:Glucose; D009584:Nitrogen