Imaging transcription in living cells.
The advent of new technologies for the imaging of living cells has made it possible to determine the properties of transcription, the kinetics of polymerase movement, the association of transcription factors, and the progression of the polymerase on the gene. We report here the current state of the field and the progress necessary to achieve a more complete understanding of the various steps in transcription. Our Consortium is dedicated to developing and implementing the technology to further this understanding.
Darzacq
Xavier
X
Janelia Farm Research Consortium on Imaging Transcription, Janelia Farm Research Campus, Howard Hughes Medical Institute, Ashburn, Virginia 20147, USA. darzacq@ens.fr
Yao
Jie
J
Larson
Daniel R
DR
Causse
Sébastien Z
SZ
Bosanac
Lana
L
de Turris
Valeria
V
Ruda
Vera M
VM
Lionnet
Timothee
T
Zenklusen
Daniel
D
Guglielmi
Benjamin
B
Tjian
Robert
R
Singer
Robert H
RH
D020869:Gene Expression Profiling / Q000379:methods*; D007090:Image Interpretation, Computer-Assisted / Q000379:methods*; D008856:Microscopy, Fluorescence / Q000379:methods*; D014157:Transcription Factors / Q000378:metabolism*; D015533:Transcriptional Activation / Q000502:physiology*
D014157:Transcription Factors