Molecules involve in the self-protection of neurons against glucose-oxygen-serum deprivation (GOSD)-induced cell damage.
Molecules involved in self-protection of neurons against glucose/oxygen/serum deprivation (GOSD) were investigated. Trypan blue dye exclusion assay, Western blotting, ELISA, cytokine antibody array and chemical blocking assay were applied in the study. Results showed that early induction (at 6h of GOSD) of cyclooxygenase-2 (COX-2), leptin, transforming growth factor-beta1 (TGF-beta1), glial-cell-line-derived neurotrophic factor (GDNF) and neurotrophin-3 (NT-3) all played a compensatory role in the protection of neurons against GOSD. Decline of these molecules and peroxisome proliferators-activated receptor (PPAR)-gamma and -alpha since 12h of GOSD may lead to an irreversible neuronal death. Nitric oxide (NO) and superoxide at low concentrations were neuroprotective whereas at high concentrations were detrimental to neurons. Accumulation of NO and superoxide at late stage of GOSD should therefore be prevented. The study provided a useful platform for screening of potential anti-ischemic drugs and also explained why GOSD neuron derived conditioned medium (NCM) only exerted a time-restricted neuroprotection.
Wang
Chen-Hsuan
CH
Department of Life Sciences, National Chung Hsing University, No. 250, Kuo Kuang Rd., Taichung 40227, Taiwan.
Lee
Wen-Jane
WJ
Ghanta
Vithal K
VK
Wang
Wei-Ti
WT
Cheng
Shu-Yun
SY
Hsueh
Chi-Mei
CM
D000818:Animals; D002470:Cell Survival; D002478:Cells, Cultured; D017077:Culture Media, Conditioned / Q000737:chemistry; D051546:Cyclooxygenase 2 / Q000378:metabolism; D051100:Glial Cell Line-Derived Neurotrophic Factor / Q000378:metabolism; D005947:Glucose / Q000378:metabolism*; D020738:Leptin / Q000378:metabolism; D009474:Neurons / Q000166:cytology / Q000378:metabolism; D018696:Neuroprotective Agents / Q000378:metabolism*; D020933:Neurotrophin 3 / Q000378:metabolism; D009569:Nitric Oxide / Q000378:metabolism; D010100:Oxygen / Q000378:metabolism*; D047492:Peroxisome Proliferator-Activated Receptors / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D044967:Serum / Q000378:metabolism*; D013481:Superoxides / Q000378:metabolism; D053773:Transforming Growth Factor beta1 / Q000378:metabolism
D017077:Culture Media, Conditioned; D051100:Glial Cell Line-Derived Neurotrophic Factor; D020738:Leptin; D018696:Neuroprotective Agents; D020933:Neurotrophin 3; D047492:Peroxisome Proliferator-Activated Receptors; D053773:Transforming Growth Factor beta1; D013481:Superoxides; D009569:Nitric Oxide; D051546:Cyclooxygenase 2; D005947:Glucose; D010100:Oxygen