Effects of bromide upon reaction of nucleosides with hydrogen peroxide induced by ultraviolet light.
When ultraviolet light was irradiated on a neutral solution of deoxynucleosides and hydrogen peroxide, concentrations of all the deoxynucleosides decreased greatly. Addition of bromide in the system suppressed the reactions of 2'-deoxycytidine, 2'-deoxythymidine, and 2'-deoxyadenosine, but not that of 2'-deoxyguanosine. Addition of hydroxyl radical scavengers suppressed the reaction. The effect of deuterium oxide, an enhancer of singlet oxygen, was small. It is reported that hydroxyl radical, generating from hydrogen peroxide by ultraviolet irradiation, can react with bromide forming bromine radical, and that bromine radical reacts with bromide forming dibromide radical anion. Our result of dose dependency of bromide suggests that dibromide radical anion is the reaction species to react only with 2'-deoxyguanosine.
School of Pharmacy, Shujitsu University, Okayama 703-8516, Japan. tsuzuki@shujitsu.ac.jp
Toshinori
T
Suzuki
Naofumi
N
Moriwaki
Kazuko
K
Kurokawa
Michiyo
M
Inukai
D001965:Bromides / Q000737:chemistry*; D003849:Deoxyguanosine / Q000737:chemistry; D003853:Deoxyribonucleosides / Q000737:chemistry*; D005609:Free Radicals / Q000737:chemistry; D006861:Hydrogen Peroxide / Q000737:chemistry / Q000528:radiation effects*; D017665:Hydroxyl Radical / Q000737:chemistry; D014466:Ultraviolet Rays
D001965:Bromides; D003853:Deoxyribonucleosides; D005609:Free Radicals; D017665:Hydroxyl Radical; D006861:Hydrogen Peroxide; D003849:Deoxyguanosine