Reduced mucosal injury of SPF chickens by mast cell stabilization after infection with very virulent infectious bursal disease virus.
Recent studies here have demonstrated that increased mast cell populations and tryptase activity contribute to lesion formation in regions of immune organs in special-pathogen-free chickens after infection with very virulent infectious bursal disease virus (vvIBDV). Mast cells and their mediators have been implicated in acute inflammatory injury after vvIBDV infection, but their precise role in this process remains elusive. In this study, the role of mast cells in the vvIBDV infection process was examined using ketotifen, a mast cell membrane stabilizer. On days 1, 2, and 3 postinfection, the bursa of Fabricius (BFs) were collected to quantify mast cells, tryptase and histamine contents by cytochemistry, immunohistochemistry and fluorospectrophotometry analyses, respectively. The results showed that the mast cell populations, tryptase expression, and histamine released increased significantly in the BFs (p<0.01) of infected birds compared to controls, and acute inflammatory responses were observed in the former. In contrast, in infected chickens pretreated with ketotifen, mast cells, tryptase, and histamine were markedly decreased (p<0.01) and probably as a result, the BFs remitted significantly. The overall results suggest that mast cells are positively involved in BF injury induced by vvIBDV infection. Inhibition of mast cell degranulation and concurrent mediator release may represent a novel strategy to modulate this process. This study, thus, advances the understanding of the acute inflammatory injury mechanisms triggered by vvIBDV infection and the contribution of mast cell activity in this process.
Wang
Decheng
D
College of Veterinary Medicine, China Agricultural University, Beijing 100193, China. wdc268@yahoo.com.cn
Liu
Yufeng
Y
She
Ruiping
R
Xu
Jingjing
J
Liu
Liqiang
L
Xiong
Jinmao
J
Yang
Yurong
Y
Sun
Quan
Q
Peng
Kaisong
K
D000818:Animals; D000956:Antigens, Viral / Q000378:metabolism; D018175:Birnaviridae Infections / Q000276:immunology / Q000473:pathology / Q000662:veterinary* / Q000821:virology; D002060:Bursa of Fabricius / Q000201:enzymology / Q000276:immunology / Q000821:virology; D002452:Cell Count; D002645:Chickens / Q000276:immunology* / Q000821:virology*; D006636:Histamine Release; D007243:Infectious bursal disease virus / Q000276:immunology / Q000472:pathogenicity*; D007249:Inflammation / Q000276:immunology / Q000473:pathology / Q000821:virology; D007665:Ketotifen / Q000494:pharmacology; D008407:Mast Cells / Q000187:drug effects / Q000201:enzymology / Q000276:immunology* / Q000473:pathology; D009092:Mucous Membrane / Q000276:immunology / Q000293:injuries / Q000473:pathology / Q000821:virology; D011201:Poultry Diseases / Q000276:immunology* / Q000473:pathology / Q000821:virology*; D013047:Specific Pathogen-Free Organisms; D053802:Tryptases / Q000378:metabolism; D014774:Virulence
D000956:Antigens, Viral; D053802:Tryptases; D007665:Ketotifen