Temperature-controlled assembly of high ordered/disordered dodecylamine layers on HOPG: consequences for DNA patterning.
It is shown that temperature-controlled ordered layers of dodecylamine, self-assembled on highly oriented pyrolytic graphite (HOPG), are an appropriate substrate for aligning individual DNA molecules. High resolution atomic force microscopy (AFM) permits visualization of the lamellar structure of dodecylamine on HOPG. The DNA adsorbed on ordered dodecylamine layers is stretched and oriented along the lamellae, while DNA on disordered dodecylamine layers is found to be in a random conformation. Small DNA bubbles appear in the case of partially denaturated linear double stranded DNA (dsDNA) adsorbed on ordered layers of dodecylamine.
Laboratoire de Physique de la Matière Vivante, Ecole Polytechnique Fédérale de Lausanne (EPFL), CH-1015 Lausanne, Switzerland.
Jozef
J
Adamcik
Susana
S
Tobenas
Giovanni
G
Di Santo
Dmitry
D
Klinov
Giovanni
G
Dietler
D000327:Adsorption; D000588:Amines / Q000737:chemistry*; D003460:Crystallization; D004247:DNA / Q000737:chemistry*; D004563:Electrochemistry / Q000379:methods; D000431:Ethanol / Q000737:chemistry; D006108:Graphite / Q000737:chemistry*; D018625:Microscopy, Atomic Force / Q000379:methods; D008968:Molecular Conformation; D015394:Molecular Structure; D010100:Oxygen / Q000737:chemistry; D012825:Silicon; D013499:Surface Properties; D013696:Temperature; D013997:Time Factors
D000588:Amines; D000431:Ethanol; D006108:Graphite; D004247:DNA; D010100:Oxygen; C003935:dodecylamine; D012825:Silicon