Ex vivo study of incorporation into adipocytes and lipolysis-inhibition effect of polycyclic aromatic hydrocarbons.
We have previously shown that benzo[a]pyrene (B[a]P) administrated at extremely low dose can cause weight gain in mice and that the increase in adipose tissue mass is due to inhibition of beta-adrenergic stimulation of lipolysis. Moreover we have suggested that in addition to its endocrine properties, adipose tissue act as a reservoir for many chemical carcinogens including Polycyclic Aromatic Hydrocarbons (PAHs). In this paper we show that B[a]P as well as the two C4 PAHs, pyrene and phenanthrene can bioaccumulate into adipocytes in a similar manner, but that at the difference of B[a]P, have no impact on epinephrine-induced lipolysis. On the basis of this ex vivo study, we therefore suggest that B[a]P may play a central role in carcinogenesis not only by inducing cancer through its mutagenic properties, but also by increasing the bioaccumulation capacity of the adipose tissue mass.
Irigaray
P
P
Cancer Research Center, Association for Research and Treatments Against Cancer (ARTAC), 57-59 rue de la convention, F-75015 Paris, France. philippei.artac@gmail.com
Lacomme
S
S
Mejean
L
L
Belpomme
D
D
D017667:Adipocytes / Q000187:drug effects* / Q000378:metabolism; D000818:Animals; D001564:Benzo(a)pyrene / Q000378:metabolism / Q000633:toxicity*; D004837:Epinephrine / Q000494:pharmacology; D005230:Fatty Acids, Nonesterified / Q000378:metabolism; D008066:Lipolysis / Q000187:drug effects*; D051379:Mice; D009153:Mutagens / Q000378:metabolism / Q000633:toxicity*; D010616:Phenanthrenes / Q000378:metabolism / Q000633:toxicity*; D011084:Polycyclic Aromatic Hydrocarbons; D011721:Pyrenes / Q000378:metabolism / Q000633:toxicity*
D005230:Fatty Acids, Nonesterified; D009153:Mutagens; D010616:Phenanthrenes; D011084:Polycyclic Aromatic Hydrocarbons; D011721:Pyrenes; D001564:Benzo(a)pyrene; C031181:phenanthrene; C030984:pyrene; D004837:Epinephrine