FT-IR spectroscopy of lipoproteins--a comparative study.
FT-IR spectra, in the frequency region 4000-600 cm(-1), of four major lipoprotein classes: very low density lipoprotein (VLDL), low density lipoprotein (LDL) and two subclasses of high density lipoproteins (HDL(2) and HDL(3)) were analyzed to obtain their detailed spectral characterization. Information about the protein domain of particle was obtained from the analysis of amide I band. The procedure of decomposition and curve fitting of this band confirms the data already known about the secondary structure of two different apolipoproteins: apo A-I in HDL(2) and HDL(3) and apo B-100 in LDL and VLDL. For information about the lipid composition and packing of the particular lipoprotein the well expressed lipid bands in the spectra were analyzed. Characterization of spectral details in the FT-IR spectrum of natural lipoprotein is necessary to study the influence of external compounds on its structure.
University of Zagreb School of Medicine, Salata 3, 10001 Zagreb, Croatia. krilov@mef.hr
Dubravka
D
Krilov
Maja
M
Balarin
Marin
M
Kosović
Ozren
O
Gamulin
Jasminka
J
Brnjas-Kraljević
D001053:Apolipoproteins / Q000032:analysis / Q000737:chemistry; D006801:Humans; D008055:Lipids / Q000032:analysis* / Q000737:chemistry; D008074:Lipoproteins / Q000032:analysis* / Q000737:chemistry; D053439:Lipoproteins, HDL2 / Q000032:analysis / Q000737:chemistry; D053440:Lipoproteins, HDL3 / Q000032:analysis / Q000737:chemistry; D008077:Lipoproteins, LDL / Q000032:analysis / Q000737:chemistry; D008079:Lipoproteins, VLDL / Q000032:analysis / Q000737:chemistry; D017433:Protein Structure, Secondary; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods*
D001053:Apolipoproteins; D008055:Lipids; D008074:Lipoproteins; D053439:Lipoproteins, HDL2; D053440:Lipoproteins, HDL3; D008077:Lipoproteins, LDL; D008079:Lipoproteins, VLDL