[FeFe] hydrogenase genetic diversity provides insight into molecular adaptation in a saline microbial mat community.
Degenerate primers for the [FeFe] hydrogenase (hydA) were developed and used in PCRs to examine hydA in microbial mats that inhabit saltern evaporative ponds in Guerrero Negro (GN), Mexico. A diversity of deduced HydA was discovered that revealed unique variants, which may reflect adaptation to the environmental conditions present in GN.
Department of Chemistry and Biochemistry and Astrobiology, Montana State University, Bozeman, 59717, USA.
Eric S
ES
Boyd
John R
JR
Spear
John W
JW
Peters
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics*; D017931:DNA Primers / Q000235:genetics; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D019015:Geologic Sediments / Q000382:microbiology*; D006864:Hydrogenase / Q000235:genetics*; D008800:Mexico; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods; D011110:Polymorphism, Genetic; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA
D001426:Bacterial Proteins; D017931:DNA Primers; D004269:DNA, Bacterial; D006864:Hydrogenase