Time-resolved spectral measurements of delayed luminescence from a single soybean seed: effects of thermal damage and correlation with germination performance.
Delayed luminescence from a single dry soybean seed was investigated in both spectral and time domains, under different excitation wavelengths. Emission spectra were collected, under 337 nm laser excitation, from native and artificially deteriorated seeds and the time-dependence of different spectral components was analyzed in detail. The single seed viability was evaluated through observation of germination properties after imbibition and compared with different parameters related to the luminescence kinetics. The significant correlation found between single seed delayed luminescence parameters and germination capability strongly validates the connection of this phenomenon with the functional state of the system and suggests the development of a non-invasive technique for seed quality determination.
Dipartimento di Metodologie Fisiche e Chimiche per l'Ingegneria, University of Catania, Catania, Italy. llanzano@uci.edu
Luca
L
Lanzanò
Li
L
Sui
Evelina
E
Costanzo
Marisa
M
Gulino
Agata
A
Scordino
Salvatore
S
Tudisco
Francesco
F
Musumeci
D018525:Germination; D006358:Hot Temperature; D049449:Luminescence; D008163:Luminescent Measurements / Q000379:methods; D012639:Seeds / Q000737:chemistry*; D013025:Glycine max / Q000737:chemistry* / Q000254:growth & development; D013696:Temperature; D013997:Time Factors