Ultraviolet A-induced peroxidation of phosphatidylcholine in unilamellar liposomes.
Unilamellar liposomes of 1-palmitoyl-2-linoleoyl-3-sn-phosphatidylcholine (PLPC) were exposed to ultraviolet A (UVA) light at 37 degrees C. The UVA-induced PLPC peroxidation was inhibited by free radical scavengers, while singlet oxygen quenchers showed slight inhibition. When pre-existing hydroperoxides were reduced, UVA irradiation could not initiate the lipid peroxidation. The UVA-induced reaction of PLPC hydroperoxides with alpha-tocopherol produced addition products. The results indicate that UVA irradiation might dissociate hydroperoxides to alkoxyl and peroxyl radicals which stimulate lipid peroxidation.
Mazari
Azzedine
A
The United Graduate School of Agricultural Science, Gifu University, Gifu, Japan.
Iwamoto
Satoshi
S
Yamauchi
Ryo
R
D002851:Chromatography, High Pressure Liquid; D006861:Hydrogen Peroxide / Q000737:chemistry / Q000494:pharmacology; D015227:Lipid Peroxidation / Q000187:drug effects / Q000528:radiation effects*; D009943:Organophosphorus Compounds / Q000494:pharmacology; D010713:Phosphatidylcholines / Q000378:metabolism*; D014466:Ultraviolet Rays / Q000009:adverse effects*; D053835:Unilamellar Liposomes / Q000378:metabolism*
D009943:Organophosphorus Compounds; D010713:Phosphatidylcholines; D053835:Unilamellar Liposomes; C061896:triphenylphosphine; C055402:1-palmitoyl-2-linoleoylphosphatidylcholine; D006861:Hydrogen Peroxide