Angiogenin is involved in lung adenocarcinoma cell proliferation and angiogenesis.
Angiogenin, a basic heparin-binding protein, has been shown to play a key role in tumor growth and angiogenesis. It was found in the present study that 67 out of 100 lung adenocarcinomas exhibited angiogenin nuclear expression, and this nuclear expression correlated with vascular and pleural invasion as well as positive lymph node metastasis. To down-regulate angiogenin expression, we constructed an adenoviral-vector based short hairpin RNA system. ELISA, real-time qPCR and immunocytochemical staining demonstrated that adenoviral-vector based siRNA decreased angiogenin mRNA level and protein secretion, and inhibited angiogenin nuclear expression in A549 cells, resulting in marked inhibition on ribosomal RNA transcription, in vitro cell proliferation, soft agar colony formation, and xenograft tumor proliferation and angiogenesis. Experiments with neomycin further confirmed that angiogenin nuclear expression played an important role in tumor growth. Based on these data, we concluded that angiogenin nuclear expression played a dual role in the growth of lung adenocarcinoma with respect to cancer cell proliferation and angiogenesis.
Laboratory of Cardiothoracic Surgery, Changhai Hospital, Shanghai 200433, PR China.
Yang
Y
Yuan
Feng
F
Wang
Xiao-Hong
XH
Liu
De-jun
DJ
Gong
He-zhong
HZ
Cheng
Sheng-dong
SD
Huang
D000230:Adenocarcinoma / Q000098:blood supply* / Q000378:metabolism* / Q000473:pathology; D000368:Aged; D043925:Angiogenesis Inducing Agents / Q000037:antagonists & inhibitors / Q000378:metabolism*; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D018450:Disease Progression; D005260:Female; D006801:Humans; D008175:Lung Neoplasms / Q000098:blood supply* / Q000378:metabolism* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D009389:Neovascularization, Pathologic / Q000378:metabolism*; D034622:RNA Interference; D012333:RNA, Messenger / Q000378:metabolism; D012259:Ribonuclease, Pancreatic / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D012270:Ribosomes / Q000378:metabolism
D043925:Angiogenesis Inducing Agents; D012333:RNA, Messenger; C046366:angiogenin; D012259:Ribonuclease, Pancreatic