[The effect of coaggregation of A.viscosus and P.gingivalis on the adherence of A.viscosus to hydroxyapatite].
OBJECTIVE
To examine the effect of coaggregation of A.viscosus and P.gingivalis on the adherence of A.viscosus to saliva-coated hydroxyapatite(S-HA).


METHODS
The suspensions of A.viscosus and P.gingivalis were rotated and dropped on cover slips to visualize the coaggregation under scanning electron microscope(SEM). Different concentrations of P.gingivalis were mixed with A.viscosus radiolabeled with 10 uCi of [methyl-(3)H] thymedine per milliliter, then rotated for periods up to 1.5 hours at room temperature and the numbers of absorbed A.viscosus cells were determined by liquid scintillation. A.viscosus alone was used as the control, and its adhesion percent was set for 100%. One-way ANOVA with SPSS10.07 software package was used to analyze the effect of P.gingivalis on adherence of A.viscosus.


RESULTS
Several cells of P.gingivalis could aggregate on the surface of A.viscosus. The adherence of A.viscosus to S-HA was promoted slightly when the concentration of P.gingivalis was rather small, then further increase in concentration of P.gingivalis resulted in a steady decline in the number of adherent A.viscosus, and the difference was significant (P<0.05).


CONCLUSIONS
These results show that the adherence of A.viscosus to S-HA could be influenced by coaggregation of P.gingivalis and A.viscosus.
Miao
Yan
Y
Department of Operative & Endodontics, Zhengzhou Stomatological Hospital, Zhengzhou 450000, Henan Province, China. miao212@126.com
Chu
Jin-Pu
JP
Wang
Hong-Feng
HF
Lou
Hong
H
Hao
Yu-Qing
YQ
D000190:Actinomyces; D001422:Bacterial Adhesion; D001439:Bacteroides; D017886:Durapatite; D012463:Saliva
D017886:Durapatite