Imaging transcription in living cells.
The advent of new technologies for the imaging of living cells has made it possible to determine the properties of transcription, the kinetics of polymerase movement, the association of transcription factors, and the progression of the polymerase on the gene. We report here the current state of the field and the progress necessary to achieve a more complete understanding of the various steps in transcription. Our Consortium is dedicated to developing and implementing the technology to further this understanding.
Janelia Farm Research Consortium on Imaging Transcription, Janelia Farm Research Campus, Howard Hughes Medical Institute, Ashburn, Virginia 20147, USA. darzacq@ens.fr
Xavier
X
Darzacq
Jie
J
Yao
Daniel R
DR
Larson
Sébastien Z
SZ
Causse
Lana
L
Bosanac
Valeria
V
de Turris
Vera M
VM
Ruda
Timothee
T
Lionnet
Daniel
D
Zenklusen
Benjamin
B
Guglielmi
Robert
R
Tjian
Robert H
RH
Singer
D020869:Gene Expression Profiling / Q000379:methods*; D007090:Image Interpretation, Computer-Assisted / Q000379:methods*; D008856:Microscopy, Fluorescence / Q000379:methods*; D014157:Transcription Factors / Q000378:metabolism*; D015533:Transcriptional Activation / Q000502:physiology*
D014157:Transcription Factors