Characterization of the RNA-binding properties of the triple-gene-block protein 2 of Bamboo mosaic virus.
The triple-gene-block protein 2 (TGBp2) of Bamboo mosaic virus (BaMV) is a transmembrane protein which was proposed to be involved in viral RNA binding during virus transport. Here, we report on the RNA-binding properties of TGBp2. Using tyrosine fluorescence spectroscopy and UV-crosslinking assays, the TGBp2 solubilized with Triton X-100 was found to interact with viral RNA in a non-specific manner. These results raise the possibility that TGBp2 facilitates intracellular delivery of viral RNA through non-specific protein-RNA interaction.
Institute of Biochemistry, National Chung-Hsing University, Taichung 40227, Taiwan, PR China. hsuhsiutingh@yahoo.com
Hsiu-Ting
HT
Hsu
Yang-Hao
YH
Tseng
Yuan-Lin
YL
Chou
Shiaw-Hwa
SH
Su
Yau-Heiu
YH
Hsu
Ban-Yang
BY
Chang
D000595:Amino Acid Sequence; D008969:Molecular Sequence Data; D017863:Potexvirus / Q000502:physiology*; D012367:RNA, Viral / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000378:metabolism*; D031787:Sasa / Q000821:virology*; D014764:Viral Proteins / Q000378:metabolism*
D012367:RNA, Viral; D016601:RNA-Binding Proteins; D014764:Viral Proteins