Full-length sequencing and genetic characterization of Breu Branco virus (Reoviridae, Orbivirus) and two related strains isolated from Anopheles mosquitoes.
Breu Branco virus (BE AR 492347) was isolated from Anopheles (Nyssorhynchus) triannulatus mosquitoes captured in Tucuruí, Pará State, northern Brazil, in 1988. No cross-reactivity by complement-fixation tests was observed between Breu Branco virus and other known arboviruses. Results of electron microscopy and physicochemical tests suggested that Breu Branco virus may be a member of the family Reoviridae. In order to elucidate its taxonomic status, a comprehensive genetic characterization was conducted for Breu Branco virus and related strains (BE AR 494475 and BE AR 486204) that were also isolated from Anopheles mosquitoes in the same area. This included full-length genome sequencing, determination of genetic traits and phylogenetic analysis. Breu Branco virus showed a similar genome organization to members of the genus Orbivirus, family Reoviridae. Genetically, Breu Branco virus was indistinguishable from strains BE AR 494475 and BE AR 486204. Phylogenetic analysis suggested that Breu Branco virus BE AR 492347 and its related strains constitute a novel species of the genus Orbivirus. Breu Branco virus is the first Brazilian orbivirus and the fifth orbivirus in the world to be sequenced fully.
Vieira
Conceição de Maria Almeida
Cde M
Amazonian Rural Federal University, Belém, Brazil.
Nunes
Marcio Roberto Teixeira
MR
da Silva
Eliana Vieira Pinto
EV
Carvalho
Valéria Lima
VL
Nunes Neto
Joaquim Pinto
JP
Cruz
Ana Cecília Ribeiro
AC
Casseb
Samir Mansour Moraes
SM
Vasconcelos
Helena Baldez
HB
Quaresma
Juarez Antônio Simões
JA
Vasconcelos
Pedro Fernando da Costa
PF
D000818:Animals; D000852:Anopheles / Q000821:virology*; D016679:Genome, Viral; D008969:Molecular Sequence Data; D018163:Orbivirus / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D010802:Phylogeny; D017422:Sequence Analysis, DNA