Molecular characterisation of kappa-5, a major antigenic glycoprotein from Schistosoma mansoni eggs.
The major immunopathological consequences of infection with Schistosoma mansoni, a T helper type 2 response and granuloma formation leading to fibrotic tissue damage, are caused by the egg stage of the parasite. Three antigens of S. mansoni eggs, termed IPSE/alpha-1, omega-1 and kappa-5, have been found to be the primary targets of the egg-directed antibody response of the host. Here, we report on the isolation, cloning and characterisation of kappa-5. Apart from an uncharacterised mRNA sequence in S. japonicum, no significant similarities of kappa-5 to known sequences from other species were found. In contrast to IPSE/alpha-1 and omega-1, which have been found only in eggs, kappa-5 was present in miracidia as well as in eggs at the mRNA and protein levels. In eggs, isoforms of kappa-5 were observed with both three and four fully occupied N-glycosylation sites, while in miracidia only one isoform with four N-glycans could be detected. Interestingly, in Western blots sera from S. mansoni-infected Africans were reactive against kappa-5 with IgE and IgG isotype antibodies, but against IPSE/alpha-1 and omega-1 only with IgG antibodies. The further characterisation of kappa-5 as one of the three major egg antigens should help to better understand the immunology and immunopathology of schistosomiasis.
Schramm
G
G
Research Center Borstel, Borstel, Germany. gschramm@fz-borstel.de
Hamilton
J V
JV
Balog
C I A
CI
Wuhrer
M
M
Gronow
A
A
Beckmann
S
S
Wippersteg
V
V
Grevelding
C G
CG
Goldmann
T
T
Weber
E
E
Brattig
N W
NW
Deelder
A M
AM
Dunne
D W
DW
Hokke
C H
CH
Haas
H
H
Doenhoff
M J
MJ
D000595:Amino Acid Sequence; D000818:Animals; D000947:Antigens, Helminth / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D001483:Base Sequence; D015153:Blotting, Western; D003001:Cloning, Molecular; D006023:Glycoproteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D006790:Host-Parasite Interactions / Q000276:immunology; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D010063:Ovum / Q000378:metabolism; D020033:Protein Isoforms; D011499:Protein Processing, Post-Translational; D012550:Schistosoma mansoni / Q000235:genetics* / Q000378:metabolism*; D012555:Schistosomiasis mansoni / Q000276:immunology
D000947:Antigens, Helminth; D006023:Glycoproteins; D020033:Protein Isoforms