Functional analysis of Pcipg2 from the straminopilous plant pathogen Phytophthora capsici.
Phytophthora capsici causes serious diseases in numerous crop plants. Polygalacturonases (PGs) are cell wall-degrading enzymes that play an important role in pathogenesis in straminopilous pathogens. To understand PGs as they relate to the virulence of P. capsici, Pcipg2 was identified from a genomic library of a highly virulent P. capsici strain. Pcipg2 was strongly expressed during symptom development after the inoculation of pepper leaves with P. capsici. The wild protein (PCIPGII) was obtained from the expression of pcipg2 and found that increasing activity of PGs in PCIPGII-treated pepper leaves was consistent with increasing symptom development. Asp residues in active sites within pcipg2 affected PCIPGII activity or its virulence on pepper leaves. Results show that pcipg2 is an important gene among pcipg genes, and illustrate the benefit of analyzing mechanisms of pathogenicity during the period of host/parasite interaction.
Department of Plant Pathology, Shandong Agricultural University, Tai'an 271018, China.
Wen Xiu
WX
Sun
Yong Jian
YJ
Jia
Bao Zhen
BZ
Feng
Nichole R
NR
O'Neill
Xiao Ping
XP
Zhu
Bing Yan
BY
Xie
Xiu Guo
XG
Zhang
D000595:Amino Acid Sequence; D001483:Base Sequence; D015152:Blotting, Northern; D015153:Blotting, Western; D002212:Capsicum / Q000382:microbiology*; D005656:Fungal Proteins / Q000235:genetics / Q000502:physiology*; D015966:Gene Expression Regulation, Fungal; D005800:Genes, Fungal; D008969:Molecular Sequence Data; D009154:Mutation; D010838:Phytophthora / Q000235:genetics / Q000472:pathogenicity / Q000502:physiology*; D018515:Plant Leaves / Q000382:microbiology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014774:Virulence
D005656:Fungal Proteins