In vitro anti-herpetic activity of an aqueous extract from the plant Phyllanthus orbicularis.
The Herpesviridae includes at least eight viral species pathogenic for humans, responsible for a wide variety of clinical symptoms. The lack of an effective vaccine and the moderate to high toxicity of the available synthetic anti-herpes compounds emphasises the need for new inhibitors. Several Phyllanthus genus (Euphorbiaceae) members have been widely used in traditional medicine and their biological properties have been intensely studied. In this study we investigated the in vitro antiviral activity of the Cuban-endemic plant Phyllanthus orbicularis H.B.K. against Herpes simplex virus type 1 (HSV-1) and 2 (HSV-2) reference strains and clinical isolates with different sensitivities to acyclovir. The inhibitory activity on Human cytomegalovirus (HCMV) replication was also investigated. The selectivity indexes (SI) found for Ph. orbicularis aqueous extract ranged from 8.7 to 37.6. Studies on the antiviral mechanisms involved revealed that the drug acted at early stages of herpesvirus replication, possibly by producing a virucidal effect, although further inhibition of intracellular replication events could not be ruled out.
Departamento de Microbiología y Virología, Facultad de Biología, Universidad de La Habana, La Habana, Cuba.
Angel L
AL
Alvarez
Gloria
G
del Barrio
Vivian
V
Kourí
Pedro A
PA
Martínez
Belén
B
Suárez
Francisco
F
Parra
D000212:Acyclovir; D000818:Animals; D000998:Antiviral Agents / Q000032:analysis*; D002522:Chlorocebus aethiops; D002853:Chromatography, Liquid; D004353:Drug Evaluation, Preclinical; D024882:Drug Resistance, Viral; D006564:Herpesviridae / Q000187:drug effects*; D006566:Herpesviridae Infections / Q000188:drug therapy; D006801:Humans; D029803:Phyllanthus / Q000737:chemistry*; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology* / Q000627:therapeutic use; D014709:Vero Cells; D014779:Virus Replication / Q000187:drug effects
D000998:Antiviral Agents; D010936:Plant Extracts; D000212:Acyclovir