Quantitative determination and comparison of the glycosaminoglycan Delta-disaccharide composition in 22 different human cell lines.
Changes in proteoglycan and glycosaminoglycan (GAG) content and distribution may play an important role in the development of many diseases, atherosclerosis, cancer and diabetes. Human cell lines act as models for the underlying pathomechanisms. Despite the importance of proteoglycans for cell functioning, information on the GAG composition of most human cell lines is limited. Comparative analysis of the GAG Deltadisaccharide amount in 22 human cell lines yielded a mean value of 94 +/- 58 pmol/10(6) cells (mean+/-SEM). Total GAG amount and heparan sulfate/heparin Deltadisaccharide composition, but not chondroitin sulfate/dermatan sulfate Deltadisaccharide composition, differed significantly between the investigated adherent and suspension cell lines. We provide a novel overview of GAG Deltadisaccharide composition in 22 different human cell lines.
Institut für Laboratoriums- und Transfusionsmedizin, Herz- und Diabeteszentrum Nordrhein-Westfalen, Universitätsklinik der Ruhr-Universität Bochum, Georgstrasse 11, 32545 Bad Oeynhausen, Germany.
Michael
M
Ambrosius
Knut
K
Kleesiek
Christian
C
Götting
D002460:Cell Line; D002851:Chromatography, High Pressure Liquid; D004187:Disaccharides / Q000032:analysis*; D006025:Glycosaminoglycans / Q000032:analysis / Q000737:chemistry*; D006801:Humans
D004187:Disaccharides; D006025:Glycosaminoglycans