Roles of the Drosophila NudE protein in kinetochore function and centrosome migration.
We examined the distribution of the dynein-associated protein NudE in Drosophila larval brain neuroblasts and spermatocytes, and analyzed the phenotypic consequences of a nudE null mutation. NudE can associate with kinetochores, spindles and the nuclear envelope. In nudE mutant brain mitotic cells, centrosomes are often detached from the poles. Moreover, the centrosomes of mutant primary spermatocytes do not migrate from the cell cortex to the nuclear envelope, establishing a new role for NudE. In mutant neuroblasts, chromosomes fail to congress to a tight metaphase plate, and cell division arrests because of spindle assembly checkpoint (SAC) activation. The targeting of NudE to mitotic kinetochores requires the dynein-interacting protein Lis1, and surprisingly Cenp-meta, a Drosophila CENP-E homolog. NudE is non-essential for the targeting of all mitotic kinetochore components tested. However, in the absence of NudE, the 'shedding' of proteins off the kinetochore is abrogated and the SAC cannot be turned off, implying that NudE regulates dynein function at the kinetochore.
Wainman
Alan
A
Instituto Pasteur Fondazione Cenci Bolognetti, Dipartimento di Genetica e Biologia Molecolare, Sapienza, Università di Roma, 00185 Rome, Italy.
Creque
Jacklyn
J
Williams
Byron
B
Williams
Erika V
EV
Bonaccorsi
Silvia
S
Gatti
Maurizio
M
Goldberg
Michael L
ML
D000818:Animals; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D002453:Cell Cycle / Q000502:physiology; D018385:Centrosome / Q000378:metabolism*; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism*; D004331:Drosophila melanogaster / Q000166:cytology / Q000235:genetics / Q000378:metabolism; D004398:Dyneins / Q000378:metabolism; D055785:Gene Knockdown Techniques; D006801:Humans; D018386:Kinetochores / Q000378:metabolism*; D008297:Male; D008540:Meiosis / Q000502:physiology; D008869:Microtubule-Associated Proteins / Q000235:genetics / Q000378:metabolism; D009154:Mutation; D009474:Neurons / Q000166:cytology / Q000502:physiology; D010641:Phenotype; D013090:Spermatocytes / Q000166:cytology / Q000378:metabolism; D008941:Spindle Apparatus / Q000378:metabolism / Q000473:pathology
D002352:Carrier Proteins; D029721:Drosophila Proteins; D008869:Microtubule-Associated Proteins; C543399:NudE protein, Drosophila; C106174:asp protein, Drosophila; C411730:cmet protein, Drosophila; D004398:Dyneins