Soil Pseudomonas community structure and its antagonism towards Rhizoctonia solani under the stress of acetochlor.
In a microcosm experiment, the amplified ribosomal DNA restriction analysis was adopted to investigate the Pseudomonas community structure in soils applied with different concentrations (0, 50, 150, and 250 mg/kg) of acetochlor, and an in vitro assay was made to examine the antagonistic activity of isolated Pseudomonas strains acting on soil-borne pathogen Rhizoctonia solani. The results showed that acetochlor application changed the community structure of Pseudomonas in aquic brown soil. The diversity of Pseudomonas and the amount of isolated Pseudomonas strains with antagonistic activity decreased with an increasing acetochlor concentration, and the toxic effect of acetochlor reached to a steady level at 150-250 mg/kg.
Key Laboratory of Terrestrial Ecological Process, Institute of Applied Ecology, Chinese Academy of Science, 110016, Shenyang, People's Republic of China.
Minna
M
Wu
Xiaoli
X
Zhang
Huiwen
H
Zhang
Yan
Y
Zhang
Xinyu
X
Li
Qixing
Q
Zhou
Chenggang
C
Zhang
D015415:Biomarkers; D016172:DNA Fingerprinting; D004269:DNA, Bacterial / Q000235:genetics; D014644:Genetic Variation; D006540:Herbicides / Q000633:toxicity*; D010944:Plants / Q000382:microbiology; D011549:Pseudomonas / Q000187:drug effects* / Q000502:physiology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012232:Rhizoctonia / Q000502:physiology*; D012988:Soil Microbiology; D014052:Toluidines / Q000633:toxicity*
D015415:Biomarkers; D004269:DNA, Bacterial; D006540:Herbicides; D014052:Toluidines; C043377:acetochlor