Determination of 7-ethoxyresorufin-o-deethylase (EROD) induction in leaping mullet (Liza saliens) from the highly contaminated Aliaga Bay, Turkey.
Pollution of the aquatic environment is a global concern owing to the devastating effects of contaminants whose levels are growing at an alarming rate, and it has become a major threat for marine organisms, as well as to humans as consumers. This study has been carried out on leaping mullet obtained from Aliaga Bay, which is located on the west coast of the Aegean Sea near Izmir and hosts the world's fifth largest shipyard, plus a broad range of industrial activities, including an oil refinery and a paper factory. The waste from these industries, combined with municipal sewer discharges, is the main cause of pollution in this region. There is no national documentation or research on the determination of pollution resulting from the industrial activities in this area. In the present study, the degree of induction of CYP4501A-associated 7-ethoxyresorufin O-deethylase (EROD) activity and immunochemical detection of CYP1A1 in the liver of leaping mullet (Lisa saliens) were used as biomarkers for the assessment of polycyclic aromatic hydrocarbon (PAH)-type organic pollutants in Aliaga Bay. Mullet caught from different locations of the bay had approximately 52 times more EROD activity than the feral fish sampled from a clean reference site near Foca, Izmir. The results of this study indicate that Aliaga Bay is highly contaminated with PAH-type organic pollutants.
Faculty of Arts & Sciences, Department of Biology, Pamukkale University, Kinikli Campus, Kinikli, Denizli, Turkey.
Alaattin
A
Sen
Onur Kenan
OK
Ulutas
Begum
B
Tutuncu
Nusret
N
Ertas
Ismet
I
Cok
D000818:Animals; D019363:Cytochrome P-450 CYP1A1 / Q000378:metabolism*; D004784:Environmental Monitoring / Q000379:methods; D004790:Enzyme Induction; D007220:Industrial Waste / Q000009:adverse effects / Q000032:analysis*; D008099:Liver / Q000187:drug effects / Q000201:enzymology; D011083:Polycyclic Compounds / Q000009:adverse effects / Q000032:analysis*; D023701:Smegmamorpha / Q000378:metabolism*; D014421:Turkey; D014874:Water Pollutants, Chemical / Q000032:analysis*
D007220:Industrial Waste; D011083:Polycyclic Compounds; D014874:Water Pollutants, Chemical; D019363:Cytochrome P-450 CYP1A1