Hypermethioninemia provokes oxidative damage and histological changes in liver of rats.
In the present study we evaluated the effect of chronic methionine administration on oxidative stress and biochemical parameters in liver and serum of rats, respectively. We also performed histological analysis in liver. Results showed that hypermethioninemia increased chemiluminescence, carbonyl content and glutathione peroxidase activity, decreased total antioxidant potential, as well as altered catalase activity. Hypermethioninemia increased synthesis and concentration of glycogen, besides histological studies showed morphological alterations and reduction in the glycogen/glycoprotein content in liver. Serum alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase and glucose were increased in hypermethioninemic rats. These findings suggest that oxidative damage and histological changes caused by methionine may be related to the hepatic injury observed in hypermethioninemia.
Stefanello
Francieli M
FM
Departamento de Bioquímica, ICBS, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil. franstefanello@yahoo.com.br
Matté
Cristiane
C
Pederzolli
Carolina D
CD
Kolling
Janaína
J
Mescka
Caroline P
CP
Lamers
Marcelo L
ML
de Assis
Adriano M
AM
Perry
Marcos L
ML
dos Santos
Marinilce F
MF
Dutra-Filho
Carlos S
CS
Wyse
Angela T S
AT
D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D001786:Blood Glucose / Q000378:metabolism; D005978:Glutathione / Q000378:metabolism; D006003:Glycogen / Q000096:biosynthesis; D006801:Humans; D008099:Liver / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism / Q000473:pathology*; D049449:Luminescence; D008715:Methionine / Q000008:administration & dosage / Q000494:pharmacology*; D018384:Oxidative Stress / Q000187:drug effects*; D051381:Rats; D017208:Rats, Wistar; D013438:Sulfhydryl Compounds / Q000378:metabolism; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
D000975:Antioxidants; D001786:Blood Glucose; D013438:Sulfhydryl Compounds; D017392:Thiobarbituric Acid Reactive Substances; D006003:Glycogen; D008715:Methionine; D005978:Glutathione