Fluorescence detection of single-nucleotide polymorphisms with a single, self-complementary, triple-stem DNA probe.
Singled out for its singularity: In a single-step, single-component, fluorescence-based method for the detection of single-nucleotide polymorphisms at room temperature, the sensor is comprised of a single, self-complementary DNA strand that forms a triple-stem structure. The large conformational change that occurs upon binding to perfectly matched (PM) targets results in a significant increase in fluorescence (see picture; F = fluorophore, Q = quencher).
Xiao
Yi
Y
Materials Department, University of California, Santa Barbara, Santa Barbara, CA 93106, USA.
Plakos
Kory J I
KJ
Lou
Xinhui
X
White
Ryan J
RJ
Qian
Jiangrong
J
Plaxco
Kevin W
KW
Soh
H Tom
HT
D001483:Base Sequence; D015342:DNA Probes / Q000737:chemistry* / Q000235:genetics; D005456:Fluorescent Dyes / Q000737:chemistry*; D020641:Polymorphism, Single Nucleotide; D013050:Spectrometry, Fluorescence; D013816:Thermodynamics
D015342:DNA Probes; D005456:Fluorescent Dyes