Lack of detection of human papillomavirus infection by hybridization test in prostatic biopsies.
OBJECTIVE
To explore the possibility of finding human papillomavirus (HPV) infection in the prostate tissue of a cohort of Saudi men presenting with benign prostatic hyperplasia (BPH) or prostate cancer.


METHODS
A cohort study on prospectively collected tissue samples was conducted at King Abdulaziz University Hospital (KAUH), Jeddah, Kingdom of Saudi Arabia from March 2007 to December 2008 on a total of 56 male patients, age range 50-93 years (average 68), diagnosed as having BPH or prostate cancer. The HPV DNA hybridization by hybrid capture 2 technology was performed on prostate biopsies of these patients to detect 18 types of HPV infection, and differentiate between 2 HPV DNA groups, the low-risk types, and the high/intermediate risk types.


RESULTS
The tissues of all the prostatic biopsies were negative for HPV DNA.


CONCLUSIONS
Our results, using the hybridization test, indicate that it is unlikely that HPV-16 or HPV-18, or the other tested subtypes, enhance the risk of prostate cancer.
Gazzaz
Faten S
FS
Medical Virology Laboratory, King Abdulaziz University Hospital, PO 80215, Jeddah 21589, Kingdom of Saudi Arabia. Fatengazzaz@hotmail.com
Mosli
Hisham A
HA
D000368:Aged; D000369:Aged, 80 and over; D001706:Biopsy; D015331:Cohort Studies; D004279:DNA, Viral / Q000302:isolation & purification; D006801:Humans; D008297:Male; D008875:Middle Aged; D027383:Papillomaviridae / Q000235:genetics / Q000302:isolation & purification*; D030361:Papillomavirus Infections / Q000175:diagnosis*; D011467:Prostate / Q000473:pathology*; D011470:Prostatic Hyperplasia / Q000473:pathology* / Q000821:virology
D004279:DNA, Viral