Improved approach for transferring and cultivating Methanosarcina acetivorans C2A (DSM 2834).
OBJECTIVE
A method for cultivating Methanosarcina acetivorans was further developed to handle these anaerobic archaea without special equipment such as an anaerobic chamber.


RESULTS
Medium was filtered and oxygen removed under a nitrogen gas-phase. A dithiothreitol-filled syringe was used to transfer cells from high density grown cultures to new medium. Growth time and cell mass were determined, as well as cell viability was proven by light microscopy.


CONCLUSIONS
Cell transfer and growth was successful using this approach.


CONCLUSIONS
This updated technique allows almost every laboratory the opportunity to grow these methanogenic organisms for further studies. The described method could be used for proteomic analysis and is also interesting for further protein structure determination.
ETH Zurich, Institute of Molecular Biology and Biophysics, CH-8093, Zurich, Switzerland. summer@ntu.edu.sg
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D003470:Culture Media / Q000378:metabolism; D046508:Culture Techniques / Q000379:methods*; D017020:Methanosarcina / Q000254:growth & development* / Q000378:metabolism; D050296:Microbial Viability; D008828:Microbiological Techniques / Q000379:methods*
D003470:Culture Media