Site-specific protein cross-linking with genetically incorporated 3,4-dihydroxy-L-phenylalanine.
Come together right now with L-DOPA: Chemical cross-linking is widely used to study protein-protein interactions. However, many cross-linking agents suffer from low reactivity or selectivity. An efficient and selective reaction of site-specific protein cross-linking was achieved using genetically incorporated 3,4-dihydroxy-L-phenylalanine.
Division of Medicinal Chemistry, College of Pharmacy, The University of Texas at Austin, 1 University Station Austin, TX 78712, USA.
Aiko
A
Umeda
Gabrielle Nina
GN
Thibodeaux
Jie
J
Zhu
YungAh
Y
Lee
Zhiwen Jonathan
ZJ
Zhang
D019881:Aminoacyltransferases / Q000378:metabolism; D001426:Bacterial Proteins / Q000378:metabolism; D018388:Codon, Terminator / Q000235:genetics* / Q000378:metabolism; D003432:Cross-Linking Reagents / Q000737:chemistry*; D003546:Cysteine Endopeptidases / Q000378:metabolism; D004295:Dihydroxyphenylalanine / Q000737:chemistry*; D050505:Mutant Proteins / Q000378:metabolism; D015202:Protein Engineering; D012333:RNA, Messenger / Q000378:metabolism; D014448:Tyrosine-tRNA Ligase / Q000378:metabolism
D001426:Bacterial Proteins; D018388:Codon, Terminator; D003432:Cross-Linking Reagents; D050505:Mutant Proteins; D012333:RNA, Messenger; D004295:Dihydroxyphenylalanine; D019881:Aminoacyltransferases; C120542:sortase A; D003546:Cysteine Endopeptidases; D014448:Tyrosine-tRNA Ligase