GnRH analogue remarkably down-regulates inflammatory proteins in peritoneal fluid proteome of women with endometriosis.
OBJECTIVE
To determine the changes in the peritoneal fluid proteome of women with endometriosis determined by the administration of gonadotropin-releasing hormone analogues (GnRH-a).


METHODS
Peritoneal fluid samples were collected during laparoscopy from patients under GnRH-a and from women who did not receive any type of hormonal treatment in the 6 months before surgery. Samples were subjected to 2-D gel electrophoresis and compared by computerized analysis. Protein spots differentially expressed between the study groups were identified by liquid chromatography tandem mass spectrometry.


RESULTS
More than 470 protein spots were analyzed. Several proteins with significant alterations were found. The down-regulated molecules were isoforms of alpha 2-HS glycoprotein, alpha 1-antitrypsin, S100-A8, haptoglobin alpha chain and vitamin D-binding protein. No protein spot had significantly higher expression in peritoneal fluid of women under GnRH-a than in untreated patients.


CONCLUSIONS
Several inflammatory molecules present in peritoneal fluid are down-regulated during treatment with GnRH-a; administration of this drug reduces the inflammation in the peritoneal cavity.
Reproductive Physiology Laboratory, St. Bartholomew's School of Medicine and Dentistry, Queen Mary and Westfield College, 48-53 St. Bartholomew's Close, St. Bartholomew's Hospital, West Smithfield, London, EC1A 7BE, United Kingdom. dr@simoneferrero.com
Simone
S
Ferrero
David John
DJ
Gillott
Valentino
V
Remorgida
Paola
P
Anserini
Nicola
N
Ragni
Jurgis Gedis
JG
Grudzinskas
D000328:Adult; D000893:Anti-Inflammatory Agents / Q000008:administration & dosage*; D001202:Ascitic Fluid / Q000737:chemistry*; D001798:Blood Proteins / Q000037:antagonists & inhibitors; D015180:Electrophoresis, Gel, Two-Dimensional; D004715:Endometriosis / Q000188:drug therapy* / Q000378:metabolism; D005260:Female; D007987:Gonadotropin-Releasing Hormone / Q000031:analogs & derivatives*; D006242:Haptoglobins / Q000037:antagonists & inhibitors; D006801:Humans; D011506:Proteins / Q000032:analysis*; D040901:Proteomics; D017329:Triptorelin Pamoate / Q000008:administration & dosage; D014809:Vitamin D-Binding Protein / Q000037:antagonists & inhibitors; D000515:alpha 1-Antitrypsin / Q000032:analysis; D060749:alpha-2-HS-Glycoprotein
C556354:AHSG protein, human; D000893:Anti-Inflammatory Agents; D001798:Blood Proteins; C485359:HP protein, human; D006242:Haptoglobins; D011506:Proteins; D014809:Vitamin D-Binding Protein; D000515:alpha 1-Antitrypsin; D060749:alpha-2-HS-Glycoprotein; D017329:Triptorelin Pamoate; D007987:Gonadotropin-Releasing Hormone