Bacterial plaque retention on oral hard materials: effect of surface roughness, surface composition, and physisorbed polycarboxylate.
Bacterial adhesion to oral hard materials is dependent on various factors, for example, surface roughness and surface composition. In this study, bacteria retention on three oral hard substrates, hydroxyapatite (HAP), enamel, and polished enamel (p-enamel) were investigated. The surface morphology and roughness of the three substrates were measured by scanning probe microscopy. HAP had the roughest surface, followed by enamel and polished enamel. For each individual substrate type, the roughness was shown to increase with scan size up to 50 microm x 50 microm. For HAP and enamel, roughness decreased considerably after formation of a pellicle, while addition of polymer coating to the pellicle layer reduced roughness much less in comparison. Bacterial surface coverage was measured at 30 min, 3 h, and 24 h on both native and surface-modified substrates, which were coated with two different polycarboxylate-based polymers, Gantrez S97 and Carbopol 940. As a result, the polymer coated surfaces had reduced bacteria coverage compared with the native surfaces over all time points and substrates measured. The reduction is the combined effect of electrostatic repulsion and sequestering of Ca(2+) ions at the surface, which plays a key role in the initial adhesion of bacteria to enamel surfaces in models of plaque formation.
Department of Materials Science and Engineering, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA.
Marla D
MD
McConnell
Yu
Y
Liu
Andrew P
AP
Nowak
Shira
S
Pilch
James G
JG
Masters
Russell J
RJ
Composto
D001422:Bacterial Adhesion / Q000502:physiology*; D020099:Coated Materials, Biocompatible; D003743:Dental Enamel / Q000737:chemistry / Q000382:microbiology / Q000648:ultrastructure; D044622:Dental Pellicle / Q000737:chemistry; D003773:Dental Plaque / Q000378:metabolism* / Q000382:microbiology; D017886:Durapatite / Q000737:chemistry*; D006801:Humans; D008422:Materials Testing; D018625:Microscopy, Atomic Force; D011076:Polycarboxylate Cement / Q000737:chemistry*; D012463:Saliva / Q000737:chemistry; D013499:Surface Properties
D020099:Coated Materials, Biocompatible; D011076:Polycarboxylate Cement; D017886:Durapatite