Phylogenetic analysis of the RNA-dependent RNA polymerase (RdRp) and a predicted structural protein (pSP) of the Chronic bee paralysis virus (CBPV) isolated from various geographic regions.
Chronic bee paralysis virus (CBPV) is responsible for chronic paralysis, an infectious and contagious disease of adult honey bees (Apis mellifera L.). The full-length nucleotide sequences of the two major RNAs of CBPV have previously been characterized. The Orf3 of RNA1 has shown significant similarities to the RNA-dependent RNA polymerase (RdRp) of positive single-stranded RNA viruses, whereas the Orf3 of RNA2 encodes a putative structural protein (pSP). In the present study, honey bees originating from 9 different countries (Austria, Poland, Hungary, Spain, Belgium, Denmark, Switzerland, Uruguay and France) were analysed for the presence of CBPV genome. The complete genomic nucleotide sequence of the RdRp (1947bp) and of the pSP (543bp) from 24 honey bee positive samples was determined and the phylogenetic relationship among isolates was investigated. Four distinct genotypes of CBPV were observed.
Blanchard
Philippe
P
Agence Française de Sécurité Sanitaire des Aliments (AFSSA), Les Templiers, Route des Chappes, BP 111, 06902 Sophia Antipolis, France. p.blanchard@afssa.fr
Schurr
Frank
F
Olivier
Violaine
V
Celle
Olivier
O
Antùnez
Karina
K
Bakonyi
Tamàs
T
Berthoud
Hélène
H
Haubruge
Eric
E
Higes
Mariano
M
Kasprzak
Sylwia
S
Koeglberger
Hemma
H
Kryger
Per
P
Thiéry
Richard
R
Ribière
Magali
M
D000818:Animals; D001317:Austria; D001516:Bees / Q000821:virology*; D001530:Belgium; D016000:Cluster Analysis; D003718:Denmark; D005602:France; D005843:Geography; D006814:Hungary; D017720:Molecular Epidemiology; D008969:Molecular Sequence Data; D010802:Phylogeny; D011044:Poland; D012324:RNA-Dependent RNA Polymerase / Q000235:genetics*; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D013030:Spain; D013557:Switzerland; D014583:Uruguay; D014764:Viral Proteins / Q000235:genetics*; D015678:Viral Structural Proteins / Q000235:genetics*; D014713:Viruses, Unclassified / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification
D014764:Viral Proteins; D015678:Viral Structural Proteins; D012324:RNA-Dependent RNA Polymerase