Characteristics of rostral solitary tract nucleus neurons with identified afferent connections that project to the parabrachial nucleus in rats.
Afferent information derived from oral chemoreceptors is transmitted to second-order neurons in the rostral solitary tract nucleus (rNST) and then relayed to other CNS locations responsible for complex sensory and motor behaviors. Here we investigate the characteristics of rNST neurons sending information rostrally to the parabrachial nucleus (PBN). Afferent connections to these rNST-PBN projection neurons were identified by anterograde labeling of the chorda tympani (CT), glossopharyngeal (IX), and lingual (LV) nerves. We used voltage- and current-clamp recordings in brain slices to characterize the expression of both the transient A-type potassium current, IKA and the hyperpolarization-activated inward current, Ih, important determinants of neuronal repetitive discharge characteristics. The majority of rNST-PBN neurons express IKA, and these IKA-expressing neurons predominate in CT and IX terminal fields but were expressed in approximately half of the neurons in the LV field. rNST-PBN neurons expressing Ih were evenly distributed among CT, IX and LV terminal fields. However, expression patterns of IKA and Ih differed among CT, IX, and LV fields. IKA-expressing neurons frequently coexpress Ih in CT and IX terminal fields, whereas neurons in LV terminal field often express only Ih. After GABAA receptor block all rNST-PBN neurons responded to afferent stimulation with all-or-none excitatory synaptic responses. rNST-PBN neurons had either multipolar or elongate morphologies and were distributed throughout the rNST, but multipolar neurons were more often encountered in CT and IX terminal fields. No correlation was found between the biophysical and morphological characteristics of the rNST-PBN projection neurons in each terminal field.
Suwabe
Takeshi
T
Department of Biologic and Materials Sciences, School of Dentistry, University of Michigan, Ann Arbor, MI 48109-1078, USA.
Bradley
Robert M
RM
D015761:4-Aminopyridine / Q000494:pharmacology; D000344:Afferent Pathways / Q000502:physiology; D000596:Amino Acids / Q000378:metabolism; D000704:Analysis of Variance; D000818:Animals; D001640:Bicuculline / Q000494:pharmacology; D055592:Biophysical Phenomena; D002772:Cholera Toxin / Q000378:metabolism; D002814:Chorda Tympani Nerve / Q000502:physiology; D004558:Electric Stimulation; D018756:GABA Antagonists / Q000494:pharmacology; D005930:Glossopharyngeal Nerve / Q000502:physiology; D066298:In Vitro Techniques; D008036:Lingual Nerve / Q000502:physiology; D008297:Male; D008564:Membrane Potentials / Q000187:drug effects / Q000502:physiology; D009474:Neurons / Q000502:physiology*; D018408:Patch-Clamp Techniques / Q000379:methods; D011149:Pons / Q000166:cytology / Q000502:physiology*; D026902:Potassium Channel Blockers / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D017552:Solitary Nucleus / Q000166:cytology* / Q000502:physiology; D055366:Synaptic Potentials / Q000187:drug effects / Q000502:physiology; D019789:Tetraethylammonium / Q000494:pharmacology
D000596:Amino Acids; D018756:GABA Antagonists; D026902:Potassium Channel Blockers; C098463:dolaisoleucine; D019789:Tetraethylammonium; D002772:Cholera Toxin; D015761:4-Aminopyridine; D001640:Bicuculline