Differentiation capacity of endothelial progenitor cells correlates with endothelial function in healthy young men.
BACKGROUND
Endothelial progenitor cells (EPCs) have been assumed to maintain vascular endothelial integrity, so the present study investigated whether the functional capacity of EPCs correlates with endothelial function in healthy young subjects, as has been confirmed in aged subjects with atherosclerotic disease.


RESULTS
EPCs in 41 healthy, young male nonsmokers (age 33.1 +/-3.9 years, mean +/- SD) were characterized. The correlation between flow-mediated vasodilation (FMD) and the number of EPCs or the plasma concentrations of growth factors, such as vascular endothelial growth factor, did not reach statistical significance. However, FMD was significantly correlated with the EPC differentiation index, defined as the ratio of the number of EPCs to the total number of adherent cells (r=0.391, P=0.011) and the abundance of endothelial nitric oxide synthase mRNA (r=0.340, P=0.030).


CONCLUSIONS
In healthy young men, despite a lack of correlation of the number or colony counts of EPCs, the ability of circulating progenitor cells to differentiate into an endothelial lineage is closely correlated with endothelial function. This cell function assay may serve as a novel biomarker for vascular function in healthy subjects in the pre-atherosclerotic stage.
Miura
Manabu
M
Department of Cardiology, Nagoya University Graduate School of Medicine, Tsurumai, Showa-ku, Nagoya, Japan.
Numaguchi
Yasushi
Y
Ishii
Masakazu
M
Kubota
Ryuji
R
Takeuchi
Toyonari
T
Imamura
Akiko
A
Murakami
Ryuichiro
R
Kondo
Takahisa
T
Okumura
Kenji
K
Murohara
Toyoaki
T
D000328:Adult; D015415:Biomarkers; D002454:Cell Differentiation; D049109:Cell Proliferation; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000166:cytology* / Q000502:physiology*; D005947:Glucose / Q000378:metabolism; D006801:Humans; D050356:Lipid Metabolism / Q000502:physiology; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism; D019001:Nitric Oxide Synthase / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D014664:Vasodilation / Q000502:physiology
D015415:Biomarkers; D012333:RNA, Messenger; D019001:Nitric Oxide Synthase; D005947:Glucose