Isolation and immunomodulatory properties of a flavonoid, casticin from Vitex agnus-castus.
Casticin (1), a flavonoid isolated from the aerial parts of Vitex agnus-castus, was found to be a potent immunomodulatory and cytotoxic compound. The activity was tested in vitro for chemiluminescence, chemotaxis, T-cell proliferation and cytotoxicity. Casticin (1) exhibited a significant inhibitory effect on monocyte oxidative burst in a dose dependent manner. It was found to have a significant suppressive effect on the chemotaxic action at higher concentrations on fMLP (10(-8) m) stimulated neutrophils. It also showed a potent suppressive effect on PHA stimulated T-cell (PMBC).
International Center for Chemical and Biological Sciences, H.E.J. Research Institute of Chemistry, Dr. Panjwani Center for Molecular Medicine and Drug Research, University of Karachi, Karachi 75270, Pakistan.
M Ahmed
MA
Mesaik
Shahnaz
S
Murad
Khalid Mohammed
KM
Khan
Rasool Bakhsh
RB
Tareen
Aqeel
A
Ahmed
Muhammad Iqbal
MI
Choudhary
D049109:Cell Proliferation / Q000187:drug effects; D002633:Chemotaxis / Q000187:drug effects; D005419:Flavonoids / Q000276:immunology / Q000302:isolation & purification / Q000494:pharmacology*; D015394:Molecular Structure; D009504:Neutrophils / Q000187:drug effects; D016897:Respiratory Burst / Q000187:drug effects; D013601:T-Lymphocytes / Q000187:drug effects; D020950:Vitex / Q000737:chemistry*
D005419:Flavonoids; C054133:casticin