Glycine transporter 1 associates with cholesterol-rich membrane raft microdomains.
Membrane rafts, the highly-ordered, cholesterol-rich microdomains of the plasma membrane play important roles in cellular functions. In this study, GLYT1-CFP and GLYT2-CFP were constructed, followed by investigation of whether the tagged transporters associate with a fluorescence probe that labels membrane rafts (DilC16) by using Fluorescence Resonance Energy Transfer. A close association was observed between DiIC16 and GLYT1-CFP, but not for GLYT2-CFP. The glycine transport ability of GLYT1 is also highly dependent on the integrity of this area. Together, the results suggest that GLYT1 and membrane rafts are co-localized in the membrane, and that this influences the rate of glycine transport.
Liu
Xin
X
Transporter Biology Group, School of Medical Sciences (Pharmacology), Bosch Institute, University of Sydney, Sydney, NSW 2006, Australia.
Mitrovic
Ann D
AD
Vandenberg
Robert J
RJ
D000818:Animals; D001692:Biological Transport; D016466:CHO Cells; D002784:Cholesterol / Q000737:chemistry / Q000378:metabolism*; D006224:Cricetinae; D003412:Cricetulus; D005998:Glycine / Q000378:metabolism*; D050487:Glycine Plasma Membrane Transport Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D021962:Membrane Microdomains / Q000737:chemistry / Q000378:metabolism*
D050487:Glycine Plasma Membrane Transport Proteins; C493271:SLC6A5 protein, human; C493268:SLC6A9 protein, human; D002784:Cholesterol; D005998:Glycine