Effects of sodium fluoride exposure on some biochemical parameters in mice: evaluation of the ameliorative effect of royal jelly applications on these parameters.
Forty eight male Balb/c mice, each weighing 30-35 g, were used in the present study. The animals were divided into four equal groups. The first group served as the control group, and the second group was administered royal jelly at a dose of 50 mg/kg bw by gavage for a period of 7 days. The third group received 200 ppm fluoride, as sodium fluoride, for a period of 7 days, in drinking water. Lastly, the fourth group was given 200 ppm fluoride in drinking water, in association with royal jelly at a dose of 50 mg/kg bw by gavage, for a period of 7 days. At the end of the seventh day, blood samples were collected from all groups into heparinised and dry tubes, and liver samples were taken concurrently. Erythrocyte and liver tissue malondialdehyde (MDA) levels and superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-Px) activities were evaluated in the blood and tissue samples obtained. Furthermore, serum cholesterol, triglyceride, glucose, total protein and albumin levels, and aspartate aminotransferase (AST), alanine aminotransferase (ALT) and alcaline phosphatase (ALP) activities were evaluated. In conclusion, fluoride was determined to cause adverse effects in mice, and the administration of royal jelly to these animals alleviated the adverse effects of fluoride.
University of Erciyes, Faculty of Veterinary Medicine, Department of Pharmacology and Toxicology, Kayseri, Turkey.
Murat
M
Kanbur
Gökhan
G
Eraslan
Sibel
S
Silici
Mürsel
M
Karabacak
D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D002374:Catalase / Q000378:metabolism; D002784:Cholesterol / Q000097:blood; D004912:Erythrocytes / Q000187:drug effects; D005227:Fatty Acids / Q000737:chemistry / Q000494:pharmacology*; D008401:Gas Chromatography-Mass Spectrometry; D005979:Glutathione Peroxidase / Q000378:metabolism; D006454:Hemoglobins / Q000378:metabolism; D008099:Liver / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism; D008297:Male; D008315:Malondialdehyde / Q000097:blood / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D018384:Oxidative Stress / Q000187:drug effects; D010636:Phenols / Q000737:chemistry / Q000494:pharmacology; D012709:Serum Albumin / Q000378:metabolism; D012969:Sodium Fluoride / Q000037:antagonists & inhibitors* / Q000633:toxicity*; D013482:Superoxide Dismutase / Q000378:metabolism; D014280:Triglycerides / Q000097:blood; D000099219:Royal Jelly
D001786:Blood Glucose; D002374:Catalase; D002784:Cholesterol; D005227:Fatty Acids; D005979:Glutathione Peroxidase; D006454:Hemoglobins; D008315:Malondialdehyde; D010636:Phenols; D012709:Serum Albumin; D012969:Sodium Fluoride; D013482:Superoxide Dismutase; D014280:Triglycerides; D000099219:Royal Jelly