[Cytogenetic characterization of the neotropical fish Brycon henni (Pisces: Characidae)].
Brycon henni, is a native fish from Western Colombia is important as food and in sport fishing, and could be cultured in waters between 18 and 28 degrees C. A previous cytogenetic study in branchial cells indicated different chromosomal complements. Total blood samples were seeded in 4 ml Ham-F12 culture medium, supplemented with 0.5 ml Bovine Fetal Serum and 0.7 ml Phyitohemaglutinin M (Gibco) during 96 hr at 28 degrees C; an antimitotic factor (100 microl Colcemid 10%) was added. Cells were incubated in 10 ml KCl hypotonic solution (0.56%) for 24 min at 37 degrees C, fixed in methanol; acetic acid (3: 1) three times, separated by centrifugations, extended drip, and the chromosomes slides were stained with 5% Giemsa (5%). The best metaphases (6 males and 8 females) were photographed. There was a diploid number of 50 chromosomes: Metacentrics (26M), Submetacentrics (16SM) and Subtelocentrics (8ST). The fundamental number is 100 arms. The evolutionary tendency is type B with no supernumerary chromosomes; a first pair of big metacentric chromosomes is characteristic for Brycon, while no chromosomic sexual heteromorfism was detected.
Grupo Biogenesis, Universidad de Antioquia, Carrera 75 N 65-87, AA 1226, Medellin, Colombia. dianahumus@yahoo.com
Diana David
DD
López
Gonzalo
G
Vásquez Palacio
Tatiana
T
Ruiz Cortés
Marta
M
Olivera Angel
D000818:Animals; D003105:Colombia; D020732:Cytogenetic Analysis / Q000379:methods*; D005260:Female; D005399:Fishes / Q000235:genetics*; D007621:Karyotyping / Q000379:methods; D008297:Male