An Assessment of Mutagenic Effect of 3, 3', 4, 4', 5 Pentachlorobiphenyl (PCB126) in Muta Mouse Fetuses.
PCBs are persistent environmental agents that induce multiple impairments in living beings. In this study we used a transgenic mouse model (Muta(TM) Mouse), carrying bacterial lacZ genes for mutation assays and for assessment of the genotoxic effect of PCB126 on fetal mice. Mothers of experimental groups were subjected to a single oral dose of PCB126 (125, 250 and 500 microg/kg) on the 10th day of pregnancy, respectively. Fetuses were autopsied on the 18th day of gestation. Cleft palate was observed in 2 out of 11 fetuses from 3 litters in 500 microg/kg treated group. Other external malformations were not observed. The DNA mutation frequencies (MF) of fetuses in each group were 1.15 +/- 0.24 x 10(-5), 0.90 +/- 0.20 x 10(-5) and 1.08 +/- 0.24 x 10(-5) in fetuses of 125, 250 and 500 microg/kg treated groups, respectively. The MF of controls was 0.81 +/- 0.22 x 10(-5). There were no significant differences among the groups. However, the MF of each treated group was a little highter than that of control group. Possible relationships between PCB and its mutagenic effects in the offspring of mice are discussed.
Inomata
Tomo
T
Department of Laboratory Animal Science, School of Veterinary Medicine, Azabu University, Japan. inomata@azabu-u.ac.jp
Sekiguchi
Midori
M
Hirayama
Shunsuke
S
Akahori
Fumiaki
F
Shirai
Mitsuyuki
M
Kashiwazaki
Naomi
N
Ito
Junya
J
Hisamatsu
Shin
S
Sakita
Katsuyasu
K
Ninomiya
Hiroyoshi
H
D000818:Animals; D001835:Body Weight / Q000187:drug effects; D002972:Cleft Palate / Q000139:chemically induced*; D004247:DNA / Q000235:genetics; D005260:Female; D005333:Fetus / Q000187:drug effects; D018811:Maternal Exposure; D051379:Mice; D008807:Mice, Inbred BALB C; D008811:Mice, Inbred DBA; D008822:Mice, Transgenic; D009152:Mutagenicity Tests / Q000379:methods / Q000662:veterinary*; D009929:Organ Size / Q000187:drug effects; D011078:Polychlorinated Biphenyls / Q000633:toxicity*; D011247:Pregnancy; D001616:beta-Galactosidase / Q000235:genetics
D004247:DNA; D011078:Polychlorinated Biphenyls; D001616:beta-Galactosidase; C023035:3,4,5,3',4'-pentachlorobiphenyl