Detection and characterization of N-glycolyated gangliosides in Wilms tumor by immunohistochemistry.
Gangliosides are glycolipids present on the cell surface. The N-glycolylated ganglioside NeuGc-GM3 has been described in some neoplasms, such as breast carcinoma and melanoma, but is usually not detected in normal human cells. Our aim was to evaluate the presence of NeuGc-GM3 in Wilms tumor by immunohistochemistry. Postchemotherapy tumors were grouped into different histologic subtypes considering the main preserved component. Formalin-fixed, paraffin-embedded tumor samples were cut into 5-microm sections. The monoclonal antibody 14F7, a mouse IgG1 that specifically recognizes NeuGc-GM3, and a peroxidase-labeled polymer conjugated to secondary antibodies were used. Sections from breast carcinoma were employed as positive controls. Presence of NeuGc-GM3 was evident in 22 of 25 (88%) cases. The staining was stronger in the epithelial component, with a membrane pattern and cytoplasmic diffusion. The stromal component expressed cytoplasmic NeuGc-GM3 in cells with rhabdomyoblastic differentiation. Tubules of adjacent renal tissue were also positive, but no expression of NeuGc-GM3 was detected in nontumoral fetal kidney. Until now, the expression of N-glycolylated gangliosides in pediatric solid tumors has not been investigated. The present study evidenced the expression of NeuGc-GM3 in a high proportion of Wilms tumors, suggesting its potential utility as a specific target of immunotherapy.
Pathology Department, Pediatric Hospital, "Prof Dr Juan P. Garrahan", Buenos Aires, Argentina.
Alejandra M
AM
Scursoni
Laura
L
Galluzzo
Sandra
S
Camarero
Norma
N
Pozzo
Mariano R
MR
Gabri
Cristina Mateo
CM
de Acosta
Ana María
AM
Vázquez
Daniel F
DF
Alonso
María Teresa G
MT
de Dávila
D000818:Animals; D014408:Biomarkers, Tumor / Q000378:metabolism; D002462:Cell Membrane / Q000378:metabolism / Q000473:pathology; D003131:Combined Modality Therapy; D004847:Epithelial Cells / Q000378:metabolism / Q000473:pathology; D019084:Fluorescent Antibody Technique, Indirect; D005679:G(M3) Ganglioside / Q000031:analogs & derivatives* / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods*; D007680:Kidney Neoplasms / Q000378:metabolism* / Q000473:pathology / Q000628:therapy; D051379:Mice; D012189:Retrospective Studies; D017154:Stromal Cells / Q000378:metabolism / Q000473:pathology; D009396:Wilms Tumor / Q000378:metabolism* / Q000473:pathology / Q000628:therapy
D014408:Biomarkers, Tumor; D005679:G(M3) Ganglioside; C044298:N-glycolylneuraminyllactosylceramide