The influences of propofol and dexmedetomidine on circadian gene expression in rat brain.
BACKGROUND
There are circadian genes whose expressions were affected by sevoflurane. We hypothesized that propofol and dexmedetomidine also have influences on the expressions of circadian genes.


METHODS
Seven-week-old rats were cannulated and propofol (600 microg/kg/min, 1 ml/h, n=6: group P6H), dexmedetomidine (1 microg/kg/min, 1 ml/h, n=6: group D6H), 10% lipid (1 ml/h, n=6: group L) and normal saline (1 ml/h, n=6: group N) were infused intravenously for 6h; rats were decapitated soon or 24h after awakening from anesthesia (groups P24H n=6 and D24H n=6). Real time reverse transcription-polymerase chain reaction was performed using rat whole brain and the expressions of circadian genes were measured.


RESULTS
In the P6H group and the P24H group, the whole expressions of seven genes were changed significantly compared with the L group. In the D6H group, the whole expression of seven genes was changed significantly compared with the N group. In the P6H group, all gene expressions except for Tef (thyrotroph embryonic factor) were changed significantly compared with the L group. In this group, the expression of Dbp (D site albumin promoter binding protein) was increased, and the others were decreased. In the D6H group, all gene expressions except for Dbp and Tef were decreased significantly compared with the N group. For the two anesthetics, the expression patterns were different only in Dbp.


CONCLUSIONS
We showed that propofol and dexmedetomidine have influences on the circadian gene expressions.
Department of Anesthesiology, Nippon Medical School, 1-1-5 Sendagi, Bunkyo-ku, Tokyo 113-8603, Japan. s7100@nms.ac.jp
Yuko
Y
Yoshida
Keiko
K
Nakazato
Ken
K
Takemori
Katsuya
K
Kobayashi
Atsuhiro
A
Sakamoto
D000704:Analysis of Variance; D000818:Animals; D050976:Basic-Leucine Zipper Transcription Factors / Q000235:genetics / Q000378:metabolism; D001921:Brain / Q000187:drug effects*; D002404:Catheterization; D018797:Cell Cycle Proteins / Q000235:genetics / Q000378:metabolism; D002940:Circadian Rhythm / Q000187:drug effects / Q000235:genetics*; D003598:Cytoskeletal Proteins / Q000235:genetics / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D020927:Dexmedetomidine / Q000494:pharmacology*; D051766:Early Growth Response Protein 1 / Q000235:genetics / Q000378:metabolism; D051767:Early Growth Response Protein 2 / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression / Q000187:drug effects*; D006993:Hypnotics and Sedatives / Q000494:pharmacology*; D008297:Male; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism; D057105:Nuclear Receptor Subfamily 4, Group A, Member 1; D056950:Period Circadian Proteins; D015742:Propofol / Q000494:pharmacology*; D051381:Rats; D011987:Receptors, Steroid / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism
D050976:Basic-Leucine Zipper Transcription Factors; D018797:Cell Cycle Proteins; D003598:Cytoskeletal Proteins; C485975:DBP protein, rat; D004268:DNA-Binding Proteins; D051766:Early Growth Response Protein 1; D051767:Early Growth Response Protein 2; C486110:Egr1 protein, rat; C497328:Egr2 protein, rat; D006993:Hypnotics and Sedatives; D009419:Nerve Tissue Proteins; C539820:Nr4a1 protein, rat; D009687:Nuclear Proteins; D057105:Nuclear Receptor Subfamily 4, Group A, Member 1; C513311:Per2 protein, rat; D056950:Period Circadian Proteins; D011987:Receptors, Steroid; D014157:Transcription Factors; C092069:activity regulated cytoskeletal-associated protein; C071247:Tef protein, rat; D020927:Dexmedetomidine; D015742:Propofol