Relationship between hemorheology and Glu(298)Asp polymorphism of endothelial nitric oxide synthase gene in patients with coronary artery disease.
This study aimed to investigate the relationship between endothelial nitric oxide synthase Glu(298)Asp gene polymorphism and hemorheological parameters. Red blood cell (RBC) deformability, aggregation were measured using an ectacytometry, whole blood, plasma viscosities were determined by a viscometer. Restriction fragment length polymorphism was used to detect polymorphism. Plasma nitrite, nitrate concentrations were determined by Griess method. The genotype distribution of the control group was as follows: 50 (67.5%) GG, 21 (28.4%) GT, 3 (4.1%) TT. A 48 (57.8%) of the patients with CAD had GG, 28 (33.7%) GT, 7 (8.5%) of them TT genotype. RBC aggregation index of CAD patients with G allele was higher and t(1/2) lower compared to controls carrying the same allele. The amplitude of RBC aggregation of healthy subjects with T allele, who are under increased cardiovascular risk was lower compared to control subjects with G allele. The results of this study indicate that, alterations in RBC aggregation seem to be a consequence of CAD, more than being a preexisting cause. Additionally, some compensatory mechanisms by causing decrements in RBC aggregation, may help regulation of circulation in healthy individuals with high cardiovascular risk.
Department of Physiology, Faculty of Medicine, Pamukkale University, Denizli, Turkey. mbor@pau.edu.tr
Melek
M
Bor-Kucukatay
Suleyman
S
Demir
Ramazan
R
Akbay
Dursun
D
Dursunoglu
Beyza
B
Akdag
Ender
E
Semiz
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000483:Alleles; D019943:Amino Acid Substitution / Q000235:genetics*; D001224:Aspartic Acid / Q000235:genetics; D016022:Case-Control Studies; D003324:Coronary Artery Disease / Q000201:enzymology* / Q000235:genetics / Q000503:physiopathology*; D004587:Electrophoresis, Agar Gel; D004903:Erythrocyte Aggregation; D005260:Female; D005787:Gene Frequency / Q000235:genetics; D005838:Genotype; D018698:Glutamic Acid / Q000235:genetics; D018056:Hemorheology / Q000235:genetics*; D006801:Humans; D008297:Male; D008875:Middle Aged; D052250:Nitric Oxide Synthase Type III / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*
D001224:Aspartic Acid; D018698:Glutamic Acid; C496314:NOS3 protein, human; D052250:Nitric Oxide Synthase Type III