Gold, poly(beta-amino ester) nanoparticles for small interfering RNA delivery.
The safe and effective delivery of RNA therapeutics remains the major barrier to their broad clinical application. Here we develop a new nanoparticulate delivery system based on inorganic particles and biodegradable polycations. First, gold nanoparticles were modified with the hydrophilic polymer poly(ethylene glycol) (PEG), and then small interfering RNA (siRNA) was conjugated to the nanoparticles via biodegradable disulfide linkages, with approximately 30 strands of siRNA per nanoparticle. The particles were then coated with a library of end-modified poly(beta-amino ester)s (PBAEs), previously identified as capable of facilitating intracellular DNA delivery. Nanoparticulate formulations developed here facilitate high levels of in vitro siRNA delivery, facilitating delivery as good or better than the commercially available lipid reagent, Lipofectamine 2000.
Department of Materials Science and Engineering, Korea University, Seoul, 136-713, Republic of Korea.
Jae-Seung
JS
Lee
Jordan J
JJ
Green
Kevin T
KT
Love
Joel
J
Sunshine
Robert
R
Langer
Daniel G
DG
Anderson
D006046:Gold / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D053768:Metal Nanoparticles / Q000737:chemistry*; D014677:Pharmaceutical Vehicles / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000008:administration & dosage*
D014677:Pharmaceutical Vehicles; D011108:Polymers; D034741:RNA, Small Interfering; C507253:poly(beta-amino ester); D006046:Gold