Content determination of aniracetam in aniracetam inclusion complex by HPLC.
We established a HPLC method for content determination of aniracetam in aniracetam inclusion complex. The chromato column was Agilent ODS (4.6mm x 150mm, 5 microm), the mobile phase was methanol-0.01 mol/L Potassium dihydrogen phosphate buffer solution (25:75, pH 3.0), with the flow rate of 1.0 ml/min, column temperature of 30 degrees and the detection wave at 280 nm, the sample size was 20microL. A good linear relationship was obtained between the peak areas and the concentrations of aniracetam in the range from 5- 80microg/ml (r=0.9998), the mean recovery was 100.1% (n=15), RSD=0.19%. This method is convenient, rapid, accurate, and brings about good recovery; it can be used for content determination of aniracetam in aniracetam inclusion complex.
Li
Yongjian
Y
Department of Pharmacy, Weifang People's Hospital, Weifang, Shandong, China.
Hu
Dejian
D
Sun
Yong
Y
D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004337:Drug Carriers / Q000737:chemistry*; D004355:Drug Stability; D016014:Linear Models; D011760:Pyrrolidinones / Q000032:analysis*; D012680:Sensitivity and Specificity; D013997:Time Factors
D004337:Drug Carriers; D011760:Pyrrolidinones; C036466:aniracetam