In vitro survival and development of goat preantral follicles in two different oxygen tensions.
The aim of the present study was to evaluate the effect of two different oxygen (O(2)) concentrations on survival and development of preantral follicles of goats cultured in vitro. Preantral ovarian follicles (> or =150 microm) were isolated from ovarian cortex fragments of goats and individually cultured for 30 days under two different O(2) concentrations (5% and 20% O(2)). Follicle development was evaluated on the basis of antral cavity formation, increase in follicular diameter, presence of healthy cumulus oocyte complexes and fully grown oocytes. Results showed with progression of culture period from 6 to 12 days, a decrease in follicular survival was observed in both O(2) concentrations (P<0.05). When the O(2) tensions were compared to each other in the different days of culture, 20% O(2) was more efficient in promoting an increase in follicular diameter from day 24 of culture onward than 5% O(2) (P<0.05). However, follicles cultured with 5% O(2) had an increased percentage of antrum formation from 12 days to the end of culture, compared with 20% O(2) (P<0.05). Moreover, there was no difference in percentage of fully developed oocytes with the different O(2) tensions. However, only oocytes (16.7%) from follicles cultured in 20% O(2) resumed meiosis. In conclusion, concentration of 20% O(2) was more efficient in promoting follicular growth and oocyte meiosis resumption from preantral follicles of goats when grown in vitro.
Faculty of Veterinary Medicine, Laboratory of Manipulation of Oocyte and Preantral Follicles (LAMOFOPA), State University of Ceará, Fortaleza, Ceará, Brazil. gomesvet@hotmail.com
C M G
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Silva
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Matos
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Rodrigues
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Faustino
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Pinto
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Chaves
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Araújo
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Campello
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Figueiredo
D000818:Animals; D005260:Female; D006041:Goats; D008540:Meiosis; D009865:Oocytes / Q000166:cytology / Q000502:physiology; D009924:Organ Culture Techniques / Q000379:methods / Q000662:veterinary*; D006080:Ovarian Follicle / Q000254:growth & development / Q000502:physiology*; D010100:Oxygen / Q000008:administration & dosage*
D010100:Oxygen