Preparation and characterization of the inclusion complex of Baicalin (BG) with beta-CD and HP-beta-CD in solution: an antioxidant ability study.
The formation of the complexes of BG with beta-CD and HP-beta-CD was studied by UV-vis absorption spectroscopy, fluorescence spectra, Phase-solubility measurements and nuclear magnetic resonance spectroscopy (NMR) in solution. The formation constants (K) of complexes were determined by fluorescence method and Phase-solubility measurements. The results showed that the inclusion ability of beta-CD and its derivatives was the order: HP-beta-CD>beta-CD. In addition, the experimental resulted confirmed the existence of 1:1 inclusion complex of BG with CDs. The antioxidant ability studies of BG and CDs complexes were done. The results obtained indicated that the BG/HP-beta-CD complex was the most reactive form, and then was the BG/beta-CD complex; the last was BG. Special configuration of complex has been proposed on NMR technique.
The Institute of Applied Chemistry of Shanxi University, Taiyuan 030006, PR China.
Jinxia
J
Li
Min
M
Zhang
Jianbin
J
Chao
Shaomin
S
Shuang
D000073738:2-Hydroxypropyl-beta-cyclodextrin; D000975:Antioxidants / Q000737:chemistry* / Q000494:pharmacology*; D001713:Biphenyl Compounds / Q000378:metabolism; D005079:Excipients / Q000737:chemistry; D005419:Flavonoids / Q000737:chemistry* / Q000494:pharmacology*; D005609:Free Radicals / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D010851:Picrates / Q000378:metabolism; D031364:Scutellaria baicalensis / Q000737:chemistry*; D012995:Solubility; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D047392:beta-Cyclodextrins / Q000737:chemistry*
D000975:Antioxidants; D001713:Biphenyl Compounds; D005079:Excipients; D005419:Flavonoids; D005609:Free Radicals; D010851:Picrates; D047392:beta-Cyclodextrins; D000073738:2-Hydroxypropyl-beta-cyclodextrin; C038044:baicalin; C004931:1,1-diphenyl-2-picrylhydrazyl