Lack of evidence for Litopenaeus vannamei Toll receptor (lToll) involvement in activation of sequence-independent antiviral immunity in shrimp.
Injection of non-specific dsRNA initiates a broad-spectrum innate antiviral immune response in the Pacific white shrimp, Litopenaeus vannamei, however, the receptor involved in recognition of this by-product of viral infections remains unknown. In vertebrates, dsRNA sensing is mediated by a class of Toll-like receptors (TLRs) and results in activation of the interferon system. Because a TLR (lToll) was recently characterized in L. vannamei, we investigated its potential role in dsRNA recognition. We showed that injection of non-specific RNA duplexes did not modify lToll gene expression. A reverse genetic approach was therefore implemented to study its role in vivo. Silencing of lToll did not impair the ability of non-specific dsRNA to trigger protection from white spot syndrome virus and did not increase the shrimp susceptibility to viral infection, when compared to controls. In contrast, gene-specific dsRNA injected to specifically silence lToll expression activated an antiviral response. These data strongly suggest that shrimp lToll plays no role in dsRNA-induced antiviral immunity.
Labreuche
Yannick
Y
Marine Biomedicine and Environmental Sciences Center, Medical University of South Carolina, Charleston, SC 29412, USA. yannick.labreuche@ifremer.fr
O'Leary
Nuala A
NA
de la Vega
Enrique
E
Veloso
Artur
A
Gross
Paul S
PS
Chapman
Robert W
RW
Browdy
Craig L
CL
Warr
Gregory W
GW
D000818:Animals; D007113:Immunity, Innate; D033561:Penaeidae / Q000235:genetics / Q000276:immunology* / Q000821:virology*; D012330:RNA, Double-Stranded / Q000276:immunology* / Q000378:metabolism; D051193:Toll-Like Receptors / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D046848:White spot syndrome virus 1 / Q000276:immunology*
D012330:RNA, Double-Stranded; D051193:Toll-Like Receptors