Rapid method for detecting resistance to a QoI fungicide in Plasmopara viticola populations.
BACKGROUND
The increasing occurrence of Qo inhibitor (QoI)-fungicide-resistant Plasmopara viticola (Berk. & MA Curtis) Berl. & DeToni populations is becoming a serious problem in the control of grapevine downy mildew worldwide.


RESULTS
The authors have developed a rapid method for detecting resistance to a QoI fungicide, azoxystrobin, in P. viticola populations using the nested PCR-RFLP method. With this method, a glycine-to-alanine substitution was discovered at codon 143 in the cytochrome b gene of P. viticola populations found in Japan.


CONCLUSIONS
It is proposed that the nested PCR-RFLP method is a high-speed, sensitive and reliable tool for detecting azoxystrobin-resistant P. viticola populations.
Furuya
Seiichi
S
The Institute of Enology and Viticulture, University of Yamanashi, Kofu, Yamanashi 400-0005, Japan.
Suzuki
Shunji
S
Kobayashi
Hironori
H
Saito
Seiya
S
Takayanagi
Tsutomu
T
D019943:Amino Acid Substitution; D045303:Cytochromes b / Q000037:antagonists & inhibitors / Q000235:genetics; D004271:DNA, Fungal / Q000032:analysis / Q000235:genetics; D025141:Drug Resistance, Fungal / Q000235:genetics; D005659:Fungicides, Industrial / Q000494:pharmacology*; D008689:Methacrylates / Q000494:pharmacology*; D009868:Oomycetes / Q000187:drug effects* / Q000235:genetics / Q000502:physiology*; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D011743:Pyrimidines / Q000494:pharmacology*; D012680:Sensitivity and Specificity; D000073739:Strobilurins; D013997:Time Factors; D027843:Vitis / Q000382:microbiology
D004271:DNA, Fungal; D005659:Fungicides, Industrial; D008689:Methacrylates; D011743:Pyrimidines; D000073739:Strobilurins; D045303:Cytochromes b; C087670:azoxystrobin