Burkholderia sp. KCTC 11096BP as a newly isolated gibberellin producing bacterium.
We isolated 864 bacteria from 553 soil samples and bioassayed them on cucumber and crown daisy for plant growth promotion. A new bacterial strain, Burkholderia sp. KCTC 11096BP gave maximum growth promotion and was selected for further investigations. The culture filtrate of this bacterium was thus analyzed for the presence of gibberellins and we found physiologically active gibberellins were found (GA(1), 0.23 ng/100 ml; GA(3), 5.11 ng/100 ml and GA(4), 2.65 ng/100 ml) along with physiologically inactive GA(9), GA(12), GA(15), GA(20), and GA(24). The bacterial isolate also solubilised tricalcium phosphate and lowered the pH of the medium during the process. The isolate was identified as a new strain of Burkholderia through phylogenetic analysis of 16S rDNA sequence. Gibberellin production capacity of genus Burkholderia is reported for the first time in current study.
Joo
Gil-Jae
GJ
Institute of Agricultural Science and Technology, Kyungpook National University, Dae-gu, 702-701, Republic of Korea.
Kang
Sang-Mo
SM
Hamayun
Muhammad
M
Kim
Sang-Kuk
SK
Na
Chae-In
CI
Shin
Dong-Hyun
DH
Lee
In-Jung
IJ
D015373:Bacterial Typing Techniques; D019117:Burkholderia / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*; D018553:Cucumis sativus / Q000254:growth & development / Q000382:microbiology; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D005875:Gibberellins / Q000096:biosynthesis*; D008969:Molecular Sequence Data; D010802:Phylogeny; D010937:Plant Growth Regulators / Q000378:metabolism*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012988:Soil Microbiology
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005875:Gibberellins; D010937:Plant Growth Regulators; D012336:RNA, Ribosomal, 16S