Bio-functionalized star PEG-coated PVDF surfaces for cytocompatibility-improved implant components.
Unmodified and GRGDS peptide-modified six arm PEG star based hydrogels (Star PEG) have been applied as a multifunctional, easy to handle coating system for textile polyvinylidene fluoride (PVDF) structures, which prevent unspecific protein and cell adsorption and control-specific cell adhesion. The reactive isocyanate-terminated Star PEG has been successfully applied to ammonia-plasma treated two- and three-dimensional PVDF surfaces. Easy modification of the surface hydrogel by mixing in of GRGDS peptide during the coating step or subsequent coupling of GRGDS was determined by TOF-SIMS. Unmodified and GRGDS-functionalized hydrogel surfaces show distinct protein repellency, as demonstrated by fluorescence microscopy after incubation with fluorescent labeled proteins and Surface MALDI-TOF-Mass Spectroscopy. Cell culture experiments with primary human dermal fibroblasts, primary fetal rat fibroblasts, and human osteoblasts on GRGDS and/or KRSR Star PEG-modified two- and three-dimensional substrates show advancement in cell adhesion and proliferation compared with untreated PVDF surfaces, whereas pure star PEG-coated surfaces show no cell adhesion. The combination of protein and cell repellent properties with specific biofunctionality and easy application of the coatings will enable their application for 3D-scaffolds.
Institute of Technical and Macromolecular Chemistry of RWTH Aachen University, Aachen, Germany.
Jean
J
Heuts
Jochen
J
Salber
Alexandra M
AM
Goldyn
Romy
R
Janser
Martin
M
Möller
Doris
D
Klee
D000327:Adsorption; D000328:Adult; D000818:Animals; D002448:Cell Adhesion; D049109:Cell Proliferation; D002478:Cells, Cultured; D020099:Coated Materials, Biocompatible / Q000737:chemistry / Q000378:metabolism; D005260:Female; D005347:Fibroblasts / Q000166:cytology / Q000502:physiology; D006801:Humans; D020100:Hydrogels / Q000737:chemistry / Q000378:metabolism; D008422:Materials Testing; D008958:Models, Molecular; D009842:Oligopeptides / Q000737:chemistry / Q000378:metabolism; D010006:Osteoblasts / Q000166:cytology / Q000502:physiology; D011092:Polyethylene Glycols / Q000737:chemistry*; D011145:Polyvinyls / Q000737:chemistry*; D019736:Prostheses and Implants; D011487:Protein Conformation; D011506:Proteins / Q000737:chemistry / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013499:Surface Properties; D005465:Fluorocarbon Polymers
D020099:Coated Materials, Biocompatible; D020100:Hydrogels; D009842:Oligopeptides; D011092:Polyethylene Glycols; D011145:Polyvinyls; D011506:Proteins; C024865:polyvinylidene fluoride; D005465:Fluorocarbon Polymers; C049554:glycyl-arginyl-glycyl-aspartyl-serine