[Construction and analysis of full-length cDNA clone of rabies virus street strain].
To construct a expression plasmid containing the full-length cDNA of rabies virus, four overlapped fragments covering full length cDNA of rabies virus street stain HN10 were cloned into pVAX1 sequentially in the genome except for the G-L noncoding region which was replaced with GFP gene. The plasmid containing the full-length viral cDNA was flanked by hammerhead ribozyme (HamRz) and hepatitis delta ribozyme (HdvRz) sequences and arranged under the control of the cytomegalovirus (CMV) promoter. The constructed plasmid could be directly used for the following procedure of producing the recombinant rabies virus street HN10.
State Key Laboratory for Molecular Virology and Genetic Engineering, Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention (IVDC, China CDC) Beijing 100052, China. pgming2002@sina.com
Ping-gang
PG
Ming
Ying
Y
Huang
Qing
Q
Tang
Jia-liang
JL
Du
Xiao-yan
XY
Tao
Jia-xin
JX
Yan
Rong-liang
RL
Hu
D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics*; D008957:Models, Genetic; D010957:Plasmids / Q000235:genetics; D011820:Rabies virus / Q000145:classification / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction
D018076:DNA, Complementary