Analysis of pralidoxime in serum, brain and CSF of rats.
After administration of various amounts of pralidoxime to rats, the levels in serum, brain and cerebrospinal fluid (CSF) were measured using capillary zone electrophoresis (CZE). The calibration curves were established using spiked samples. The calibration covers the ranges from 0.3 - 200 microg/mL, 0.3 - 7 microg/mL and 0.1 - 7 microg/mL for serum, brain and CSF, respectively. The CZE measurement opens the way to the fast and reliable determination of pyridinium aldoxime concentrations in serum, cerebrospinal fluid and brain, thereby monitoring blood-brain and blood-CSF penetration of pyridinium aldoxime-type antidotes clinically used in organophosphate poisoning.
Department of Pharmacology & Therapeutics, Faculty of Medicine & Health Sciences, United Arab Emirates University, Al Ain, United Arab Emirates. drkalasz@gmail.com
Huba
H
Kalász
Eva
E
Szöko
Tamás
T
Tábi
Georg A
GA
Petroianu
Dietrich E
DE
Lorke
Abdulrab
A
Omar
Salem
S
Alafifi
Almerri
A
Jasem
Kornélia
K
Tekes
D000818:Animals; D000931:Antidotes / Q000032:analysis* / Q000493:pharmacokinetics; D001812:Blood-Brain Barrier / Q000378:metabolism; D001923:Brain Chemistry; D004305:Dose-Response Relationship, Drug; D019075:Electrophoresis, Capillary; D008297:Male; D011220:Pralidoxime Compounds / Q000032:analysis / Q000097:blood* / Q000134:cerebrospinal fluid* / Q000493:pharmacokinetics; D051381:Rats; D017208:Rats, Wistar
D000931:Antidotes; D011220:Pralidoxime Compounds; C028797:pralidoxime