Limited antioxidant effect after consumption of a single dose of tomato sauce by young males, despite a rise in plasma lycopene.
This study investigated the effect of a single dose of tomato sauce on healthy male volunteers in a randomized crossover study. Healthy male subjects (n = 10) were enrolled. Placebo (rice and olive oil) or tomato (tomato sauce, rice and olive oil) meals were provided to the volunteers. Blood and urine samples were taken before consumption of meal (0 h) and 2, 4, 6, 24 and 48 h after meal. Consumption of tomato sauce increased plasma lycopene level by 5-22%, with a maximum level at 24 h (p<0.01) after the meal. Levels of plasma F(2)-isoprostanes, hydroxyeicosatetraenoic acid products, allantoin and urinary 8-hydroxy-2'-deoxyguanosine did not change after either meal, but urinary F(2)-isoprostanes (p<0.05) significantly decreased at 48 h compared to 0 h after the tomato sauce meal. This study showed that a single dose of tomato sauce meal had only a limited antioxidant effect in vivo.
Department of Biochemistry, Yong Loo Lin School of Medicine, National University of Singapore, Singapore.
Chung-Yung Jetty
CY
Lee
Helen B
HB
Isaac
Shan Hong
SH
Huang
Lee Hua
LH
Long
Huansong
H
Wang
Jan
J
Gruber
Choon Nam
CN
Ong
Ronan P
RP
Kelly
Barry
B
Halliwell
D000328:Adult; D000481:Allantoin / Q000097:blood; D000975:Antioxidants / Q000008:administration & dosage*; D015415:Biomarkers / Q000032:analysis*; D002338:Carotenoids / Q000097:blood*; D002851:Chromatography, High Pressure Liquid; D018592:Cross-Over Studies; D003839:Deoxyadenosines / Q000652:urine; D004032:Diet; D028441:F2-Isoprostanes / Q000097:blood / Q000652:urine; D006801:Humans; D006893:Hydroxyeicosatetraenoic Acids / Q000097:blood; D000077276:Lycopene; D018551:Solanum lycopersicum; D008297:Male
C436623:8-hydroxy-2'-deoxyadenosine; D000975:Antioxidants; D015415:Biomarkers; D003839:Deoxyadenosines; D028441:F2-Isoprostanes; D006893:Hydroxyeicosatetraenoic Acids; D000481:Allantoin; D002338:Carotenoids; D000077276:Lycopene