Preparation and characterization of fibroin/hyaluronic acid composite scaffold.
Hyaluronic acid (HA) was added into fibroin solution to prepare fibroin-based porous composite scaffolds. HA exhibited important effects on pore formation and hydrophilicity of fibroin-based scaffold. The aqueous-fibroin/HA scaffolds had highly homogeneous and interconnected pores with porosity of above 90% and controllable pore size ranging from 123 to 253 microm. The water take-up of fibroin/HA scaffolds increased significantly with the increase of HA content. Containing HA at a defined content range, such as 3-6%, fibroin-based scaffolds' affinity to primary neural cells was improved. In 6%HA/fibroin scaffolds, neurosphere-forming cell migrated from their original aggregate and adhered tightly to the surface of scaffolds.
Biomaterials Laboratory, Department of Materials Science and Engineering, Tsinghua University, Beijing 100084, People's Republic of China.
Yong-Juan
YJ
Ren
Zi-You
ZY
Zhou
Bing-Fang
BF
Liu
Qun-Yuan
QY
Xu
Fu-Zhai
FZ
Cui
D000818:Animals; D001696:Biomechanical Phenomena; D012831:Bombyx; D002448:Cell Adhesion / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D005260:Female; D005349:Fibroins / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology*; D006820:Hyaluronic Acid / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology*; D057927:Hydrophobic and Hydrophilic Interactions; D019476:Insect Proteins / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D008855:Microscopy, Electron, Scanning; D009474:Neurons / Q000166:cytology / Q000187:drug effects; D016062:Porosity / Q000187:drug effects; D011247:Pregnancy; D051381:Rats; D017207:Rats, Sprague-Dawley; D017550:Spectroscopy, Fourier Transform Infrared; D013234:Stem Cells / Q000166:cytology / Q000187:drug effects; D023822:Tissue Engineering; D054457:Tissue Scaffolds / Q000737:chemistry*; D014867:Water / Q000737:chemistry
D019476:Insect Proteins; D014867:Water; D006820:Hyaluronic Acid; D005349:Fibroins