In vitro antiplasmodial activity of plants used in Benin in traditional medicine to treat malaria.
OBJECTIVE
The aim of the study was to evaluate the in vitro antiplasmodial activity of crude extracts of 12 plant species traditionally used in Benin for the treatment of malaria in order to validate their use.


METHODS
For each species, dichloromethane, methanol and total aqueous extracts were tested. The antiplasmodial activity of extracts was evaluated using the measurement of the plasmodial lactate dehydrogenase activity on chloroquine-sensitive (3D7) and resistant (W2) strains of Plasmodium falciparum. The selectivity of the different extracts was evaluated using the MTT test on J774 macrophage-like murine cells and WI38 human normal fibroblasts.


RESULTS
The best growth inhibition of both strains of Plasmodium falciparum was observed with the dichloromethane extracts of Acanthospermum hispidum DC. (Asteraceae) (IC(50)=7.5 microg/ml on 3D7 and 4.8 microg/ml on W2), Keetia leucantha (K. Krause) Bridson (syn. Plectronia leucantha Krause) (Rubiaceae) leaves and twigs (IC(50)=13.8 and 11.3 microg/ml on 3D7 and IC(50)=26.5 and 15.8 microg/ml on W2, respectively), Carpolobia lutea G.Don. (Polygalaceae) (IC(50)=19.4 microg/ml on 3D7 and 8.1 microg/ml on W2) and Strychnos spinosa Lam. (Loganiaceae) leaves (IC(50)=15.6 microg/ml on 3D7 and 8.9 microg/ml on W2). All these extracts had a low cytotoxicity.


CONCLUSIONS
Our study gives some justifications for the traditional uses of some investigated plants.
Bero
Joanne
J
Louvain Drug Research Institute, Université catholique de Louvain, UCL 7230, Brussels, Belgium. joanne.bero@uclouvain.be
Ganfon
Habib
H
Jonville
Marie-Caroline
MC
Frédérich
Michel
M
Gbaguidi
Fernand
F
DeMol
Patrick
P
Moudachirou
Mansourou
M
Quetin-Leclercq
Joëlle
J
D000818:Animals; D000962:Antimalarials / Q000494:pharmacology* / Q000627:therapeutic use; D001541:Benin; D002460:Cell Line; D006801:Humans; D019684:Magnoliopsida; D008288:Malaria / Q000188:drug therapy / Q000469:parasitology*; D008519:Medicine, Traditional; D051379:Mice; D021261:Parasitic Sensitivity Tests; D008517:Phytotherapy; D010936:Plant Extracts / Q000009:adverse effects / Q000494:pharmacology* / Q000627:therapeutic use; D010946:Plants, Medicinal; D010963:Plasmodium falciparum / Q000187:drug effects*
D000962:Antimalarials; D010936:Plant Extracts