[Investigation of conjugation and metabolism of propofol with the method of high performance liquid chromatography].
Propophol (somnopol, diprivan) is widely used in medicine for local and general narcosis through single injection or permanent infusion. Study of conjugation and metabolism of medications is considered as question of great importance, especially for substances used at anaesthesia. To study the above-mentioned questions the total complex of methods of chromatographical analysis, especially, high performance liquid chromatolgraphy (HPLC), are widely used. Objects of investigation were: blood plasma and urine of surgical patients. Method of investigation - HPLC with Ultraviolet-Detector. For investigation of structures of propophol conjugates and its metabolites selective enzymatic and non-selective acid hydrolysis were used. Two metabolites: 2,6-diisoprophil-1,4-bensoxinon and 2,6-diisopropilhydroxinon were determined.
Adeishvili-Andguladze
L V
LV
Makharadze
R V
RV
Kurtsikidze
M Sh
MSh
Abuladze
N B
NB
Gabuniia
K U
KU
D018686:Anesthetics, Intravenous / Q000737:chemistry / Q000493:pharmacokinetics*; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D015742:Propofol / Q000031:analogs & derivatives / Q000032:analysis / Q000737:chemistry / Q000493:pharmacokinetics*
D018686:Anesthetics, Intravenous; D015742:Propofol