Exploring the impact of osmoadaptation on glycolysis using time-varying response-coefficients.
We present a model of osmoadaptation in S. cerevisiae based on existing experimental and theoretical work. In order to investigate the impact of osmoadaptation on glycolysis, this model focuses on the interactions between glycolysis and osmoadaptation, namely the production of glycerol and its influence on flux towards pyruvate. Evaluation of this model shows that, depending on initial relations between glycerol and pyruvate production, the increased glycerol production can have a substantial negative effect on the pyruvate production rate. Existing experimental data and a detailed analysis of the model lead to the suggestion of an interaction between activated Hog1 and activators of glycolysis such as Pfk26.
Computational Systems Biology Group, Max Planck Institute for Molecular Genetics, Ihnestrasse 63-73, 14195 Berlin, Germany. kuehn@molgen.mpg.de
Clemens
C
Kühn
Elzbieta
E
Petelenz
Bodil
B
Nordlander
Jörg
J
Schaber
Stefan
S
Hohmann
Edda
E
Klipp
D003198:Computer Simulation; D004798:Enzymes / Q000235:genetics / Q000378:metabolism; D015966:Gene Expression Regulation, Fungal; D005990:Glycerol / Q000378:metabolism; D006019:Glycolysis / Q000235:genetics*; D007700:Kinetics; D008954:Models, Biological; D008957:Models, Genetic; D008967:Molecular Biology / Q000379:methods; D012331:RNA, Fungal / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction; D014158:Transcription, Genetic
D004798:Enzymes; D012331:RNA, Fungal; D012333:RNA, Messenger; D029701:Saccharomyces cerevisiae Proteins; D005990:Glycerol