Light-scattering spectroscopy differentiates fetal from adult nucleated red blood cells: may lead to noninvasive prenatal diagnosis.
Present techniques for prenatal diagnosis are invasive and present significant risks of fetal loss. Noninvasive prenatal diagnosis utilizing fetal nucleated red blood cells (fNRBC) circulating in maternal peripheral blood has received attention, since it poses no risk to the fetus. However, because of the failure to find broadly applicable identifiers that can differentiate fetal from adult NRBC, reliable detection of viable fNRBC in amounts sufficient for clinical use remains a challenge. In this Letter we show that fNRBC light-scattering spectroscopic signatures may lead to a clinically useful method of minimally invasive prenatal genetic testing.
Biomedical Imaging and Spectroscopy Laboratory, Department of ObGyn and Reproductive Biology, Beth Israel Deaconess Medical Center, Harvard University, Boston, Massachusetts 02215, USA.
Kee-Hak
KH
Lim
Saira
S
Salahuddin
Le
L
Qiu
Hui
H
Fang
Edward
E
Vitkin
Ionita C
IC
Ghiran
Mark D
MD
Modell
Tamara
T
Takoudes
Irving
I
Itzkan
Eugene B
EB
Hanlon
Benjamin P
BP
Sachs
Lev T
LT
Perelman
D000328:Adult; D004900:Erythroblasts / Q000166:cytology; D004912:Erythrocytes / Q000166:cytology*; D005260:Female; D005333:Fetus / Q000166:cytology*; D006801:Humans; D008027:Light; D011247:Pregnancy; D011296:Prenatal Diagnosis / Q000379:methods*; D012542:Scattering, Radiation; D013057:Spectrum Analysis / Q000379:methods*