[FeFe] hydrogenase genetic diversity provides insight into molecular adaptation in a saline microbial mat community.
Degenerate primers for the [FeFe] hydrogenase (hydA) were developed and used in PCRs to examine hydA in microbial mats that inhabit saltern evaporative ponds in Guerrero Negro (GN), Mexico. A diversity of deduced HydA was discovered that revealed unique variants, which may reflect adaptation to the environmental conditions present in GN.
Boyd
Eric S
ES
Department of Chemistry and Biochemistry and Astrobiology, Montana State University, Bozeman, 59717, USA.
Spear
John R
JR
Peters
John W
JW
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics*; D017931:DNA Primers / Q000235:genetics; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D019015:Geologic Sediments / Q000382:microbiology*; D006864:Hydrogenase / Q000235:genetics*; D008800:Mexico; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods; D011110:Polymorphism, Genetic; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA
D001426:Bacterial Proteins; D017931:DNA Primers; D004269:DNA, Bacterial; D006864:Hydrogenase