Glycine transporter 1 associates with cholesterol-rich membrane raft microdomains.
Membrane rafts, the highly-ordered, cholesterol-rich microdomains of the plasma membrane play important roles in cellular functions. In this study, GLYT1-CFP and GLYT2-CFP were constructed, followed by investigation of whether the tagged transporters associate with a fluorescence probe that labels membrane rafts (DilC16) by using Fluorescence Resonance Energy Transfer. A close association was observed between DiIC16 and GLYT1-CFP, but not for GLYT2-CFP. The glycine transport ability of GLYT1 is also highly dependent on the integrity of this area. Together, the results suggest that GLYT1 and membrane rafts are co-localized in the membrane, and that this influences the rate of glycine transport.
Transporter Biology Group, School of Medical Sciences (Pharmacology), Bosch Institute, University of Sydney, Sydney, NSW 2006, Australia.
Xin
X
Liu
Ann D
AD
Mitrovic
Robert J
RJ
Vandenberg
D000818:Animals; D001692:Biological Transport; D016466:CHO Cells; D002784:Cholesterol / Q000737:chemistry / Q000378:metabolism*; D006224:Cricetinae; D003412:Cricetulus; D005998:Glycine / Q000378:metabolism*; D050487:Glycine Plasma Membrane Transport Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D021962:Membrane Microdomains / Q000737:chemistry / Q000378:metabolism*
D050487:Glycine Plasma Membrane Transport Proteins; C493271:SLC6A5 protein, human; C493268:SLC6A9 protein, human; D002784:Cholesterol; D005998:Glycine