[Application of liquid chromatography coupled with mass spectrometry (LC/MS) to determine antidepressants in blood samples].
A simultaneous determination of twelve antidepressant drugs (amitriptyline, chlorpromazine, clomipramine, doxepin, fluoxetine, levomepromazine, maprotiline, paroxetine, perazine, promazine, sertraline, thioridazine) in blood samples was developed using high-performance liquid chromatography with mass spectrometry (LC/MS). Blood samples were extracted with acetonitrile and separated by HPLC on a reversed phase C18 column with a mobile phase consisting of acetonitrile -0.1% trifluoroacetic acid (50:50 (v/v) at a flow-rate of 0.4 ml/min. The analytes were determined using electrospray ionization in a single quadrupole mass spectrometer. LC-ESI-MS was performed in the selected-ion monitoring (SIM) mode using target ions. The validated method was successfully used to analyze antidepressants in blood samples collected from four victims of suicide by hanging.
Katedry Medycyny Sadowej UMK w Toruniu, Collegium Medicum im. Ludwika Rydygiera w Bydgoszczy.
Ewa
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Pufal
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Sykutera
D000929:Antidepressive Agents, Tricyclic / Q000097:blood*; D001774:Blood Chemical Analysis; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005554:Forensic Medicine / Q000379:methods*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006801:Humans; D007202:Indicators and Reagents / Q000737:chemistry; D011044:Poland; D012680:Sensitivity and Specificity; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
D000929:Antidepressive Agents, Tricyclic; D007202:Indicators and Reagents