Morphological investigations of cells that adhered to the irregular patterned polydimethylsiloxane (PDMS) surface without reagents.
Polydimethylsiloxane (PDMS) surface consisting irregular pattern was investigated to develop cell-based biochip using PDMS. PDMS surface was modified with nano- and micro-combined patterns using surface deformation technology. Hydrophobicity of nano-patterned PDMS surface was sustained. Nevertheless it has irregular patterns consisting of micro- and nano-patterns. According to atomic force microscopy (AFM), scanning electron microscopy (SEM) and confocal microscopy results by immunostaining method, human mammary epithelial cells (HMEC) adhered well on irregularly patterned surface without any reagents such as gelatin and collagen, compared to commercial culture dish. It implies PDMS material can be utilized as template for cell-based biochip without any reagents.
Chung
Sung Hee
SH
Department of Chemical & Biomolecular Engineering, Sogang University, Seoul 121-742, Republic of Korea.
Min
Junhong
J
D002448:Cell Adhesion; D002460:Cell Line; D004129:Dimethylpolysiloxanes / Q000378:metabolism*; D004847:Epithelial Cells / Q000502:physiology*; D006801:Humans; D018625:Microscopy, Atomic Force; D018613:Microscopy, Confocal; D008855:Microscopy, Electron, Scanning
D004129:Dimethylpolysiloxanes; C013830:baysilon