Mesenchymal stem cells showed the highest potential for the regeneration of injured liver tissue compared with other subpopulations of the bone marrow.
We have previously reported that bone marrow cells (BMCs) participate in the regeneration after liver injury. However, it is not established that this is the result of differentiation of hematopoietic stem cells (HSCs), mesenchymal stem cells (MSCs) or the combination of both. We investigated the contribution of each cell fraction to the regenerative process. First, we confirmed that transplanted stem cells migrate directly to injured liver tissue without dispersing to other organs. Next, we divided green fluorescent protein (GFP)-expressing BMCs into three populations as mononuclear cells, MSCs and HSCs. We then compared the engraftment capacity after transplantation of each fraction of cells into liver-injured mice. Of these, the MSCs transplanted group showed the highest GFP fluorescence intensities in liver tissue by flow cytometry analysis and confocal microscopic observation. Furthermore, MSCs showed differentiation potential into hepatocytes when co-cultured with injured liver cells, which suggests that MSCs showed highest potential for the regeneration of injured liver tissue compared with those of the other two cell refractions.
Cho
Kyung-Ah
KA
Department of Microbiology, Ewha Womans University College of Medicine, Ewha Medical Research Center, Seoul, Republic of Korea.
Ju
Sun-Young
SY
Cho
Su Jin
SJ
Jung
Yun-Jae
YJ
Woo
So-Youn
SY
Seoh
Ju-Young
JY
Han
Ho-Seong
HS
Ryu
Kyung-Ha
KH
D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology*; D002251:Carbon Tetrachloride / Q000633:toxicity; D056486:Chemical and Drug Induced Liver Injury; D018920:Coculture Techniques; D005260:Female; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D022781:Hepatocytes / Q000166:cytology; D008107:Liver Diseases / Q000628:therapy*; D008115:Liver Regeneration / Q000502:physiology*; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic
D049452:Green Fluorescent Proteins; D002251:Carbon Tetrachloride