Association between color doppler vascularity index, angiogenesis-related molecules, and clinical outcomes in gastric cancer.
OBJECTIVE
This study was conducted to evaluate the correlation between color Doppler vascularity index (CDVI), clinical outcomes and five angiogenesis-related molecules including vascular endothelial growth factor (VEGF), placenta growth factor (PlGF), cyclooxygenase-2 (COX-2), inducible nitric oxide synthase (iNOS), and calreticulin (CRT) in gastric cancer, and to develop an effective model selected from these five molecules to predict patient survival.


METHODS
CDVI could be obtained preoperatively by transabdominal ultrasound from 30 patients. Enzyme immunoassay was adopted to determine protein level of VEGF and PlGF, and immunohistochemistry was used to detect COX-2, iNOS and CRT expression. Correlation between CDVI and five individual molecules was assessed. Multiple molecules model was developed using classification and regression tree (CART) analysis from five molecules, and was tested for patient survival in another 45 patients.


RESULTS
CDVI was significantly correlated with patient survival (P = 0.00907) and absolute number of metastatic lymph nodes (P = 0.01). There was no significant association between CDVI and any individual molecule. The model, developed by CART consisting of VEGF and PlGF, could differentiate high and low CDVI and survival in testing group (P = 0.00257).


CONCLUSIONS
CDVI was associated with lymph node metastasis, combined VEGF and PlGF expression status and patient survival in gastric cancer.
Department of Surgery, National Taiwan University Hospital, No. 7, Chung-Shan South Road, Taipei, Taiwan. cnchen@ntu.edu.tw
Chiung-Nien
CN
Chen
Jen-Jen
JJ
Lin
Hsinyu
H
Lee
Yung-Ming
YM
Cheng
King-Jen
KJ
Chang
Fon-Jou
FJ
Hsieh
Hong-Shiee
HS
Lai
Chien-Cheng
CC
Chang
Po-Huang
PH
Lee
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015415:Biomarkers / Q000097:blood; D005260:Female; D006801:Humans; D008207:Lymphatic Metastasis; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D009389:Neovascularization, Pathologic / Q000097:blood*; D012189:Retrospective Studies; D013274:Stomach Neoplasms / Q000097:blood / Q000098:blood supply* / Q000000981:diagnostic imaging / Q000401:mortality / Q000473:pathology; D016019:Survival Analysis; D018615:Ultrasonography, Doppler, Color
D015415:Biomarkers