Induced proteome of Trichoderma harzianum by Botrytis cinerea.
As a notable biocontrol agent, Trichoderma harzianum can antagonize a diverse array of phytopathogenic fungi, including Botrytis cinerea, Rhizoctonia solani and Fusarium oxysporum. Elucidating the biocontrol mechanism of T. harzianum in response to the pathogens enables it to be exploited in the control of plant diseases. Two-dimensional gel electrophoresis (2-DE) was performed to obtain secreted protein patterns of T. harzianum ETS 323, grown in media that contained glucose, a mixture of glucose and deactivated B. cinerea mycelia, deactivated B. cinerea mycelia or deactivated T. harzianum mycelia. Selected protein spots were identified using liquid chromatography-tandem mass spectrometry (LC-MS/MS). Ninety one out of 100 excised protein spots were analyzed and some proteins were sequence identified. Of these, one l-amino acid oxidase (LAAO) and two endochitinases were uniquely induced in the media that contained deactivated B. cinerea mycelia as the sole carbon source. Activities of the cell wall-degrading enzymes (CWDEs), including beta-1,3-glucanases, beta-1,6-glucanases, chitinases, proteases and xylanases, were significantly higher in media with deactivated B. cinerea mycelia than in other media. This finding suggests that the cell wall of B. cinerea is indeed the primary target of T. harzianum ETS 323 in the biocontrol mechanism. The possible roles of LAAO and xylanase were also discussed.
Department of Research, Buddhist Tzu Chi General Hospital, General Education Center, Tzu Chi College of Technology, 97002 Hualien, Taiwan.
Hsueh-Hui
HH
Yang
Siwy Ling
SL
Yang
Kou-Cheng
KC
Peng
Chaur-Tsuen
CT
Lo
Shu-Ying
SY
Liu
D020171:Botrytis / Q000378:metabolism*; D002688:Chitinases / Q000737:chemistry / Q000378:metabolism; D015180:Electrophoresis, Gel, Two-Dimensional; D005656:Fungal Proteins / Q000737:chemistry / Q000378:metabolism; D005670:Fusarium / Q000378:metabolism; D043326:Glucan 1,3-beta-Glucosidase / Q000737:chemistry / Q000378:metabolism; D050830:L-Amino Acid Oxidase / Q000737:chemistry / Q000378:metabolism; D010572:Pest Control, Biological / Q000379:methods; D010935:Plant Diseases / Q000382:microbiology; D020543:Proteome / Q000737:chemistry / Q000378:metabolism*; D012232:Rhizoctonia / Q000378:metabolism; D014242:Trichoderma / Q000737:chemistry / Q000201:enzymology*; D014995:Xylosidases / Q000737:chemistry / Q000378:metabolism
D005656:Fungal Proteins; D020543:Proteome; D050830:L-Amino Acid Oxidase; D014995:Xylosidases; D002688:Chitinases; D043326:Glucan 1,3-beta-Glucosidase