Mimicking vernix caseosa--preparation and characterization of synthetic biofilms.
The multiple protecting and barrier-supporting properties of the creamy, white biofilm vernix caseosa (VC) before and after birth suggest that a VC biomimetic could be an innovative barrier cream for barrier-deficient skin. The aim of this study was the rational design and preparation of synthetic biofilms mimicking the unique composition and properties of natural VC. Hexagonal, highly hydrated hyperbranched polyglycerol microgel particles (30 microm in diameter) were embedded in a synthetic lanolin-based lipid mixture using a micromixer. In these formulations, the water content of the particles (i.e. 50% and 80%), an additional lipid coating of the particles and different particle/lipid ratios were varied. Characterization with confocal laser scanning microscopy (CLSM) showed a homogeneous distribution of the labeled particles in the lipid matrix. Regarding structural appearance, particle density and distribution, the formulations with a high particle/lipid ratio (5:1) resembled native VC very closely. Comparable results between native VC and the synthetic formulations were obtained concerning water handling properties, thermotropic behavior while lower elasticity and lower viscosity were observed for the synthetic biofilms. The biofilm formulations were stable for at least 1 month at 4 degrees C. In conclusion, our formulations mimic natural VC very closely and are promising candidates for in vivo studies.
Department of Drug Delivery Technology, Leiden/Amsterdam Center for Drug Research, Leiden University, Leiden, The Netherlands.
Robert
R
Rissmann
Marion H M
MH
Oudshoorn
Raphaël
R
Zwier
Maria
M
Ponec
Joke A
JA
Bouwstra
Wim E
WE
Hennink
D018441:Biofilms; D040761:Biomimetic Materials / Q000032:analysis / Q000138:chemical synthesis*; D032701:Biomimetics / Q000379:methods*; D008567:Membranes, Artificial; D014708:Vernix Caseosa / Q000737:chemistry / Q000502:physiology
D008567:Membranes, Artificial