Presence of necrotic strains of Potato virus Y in Mexican potatoes.
As part of a routine screening for the possible presence of the necrotic strains of potato virus Y affecting potatoes in Mexico, five PVY isolates were submitted to biological and molecular analysis. Considering their serological properties, two belong to the common strain (O) and three to the necrotic strain (N). All the isolates induced vein necrosis in Nicotiana tabacum. To characterize the isolates, 5' NTR and P1 genes were sequenced and compared with sequences from GenBank database. Nucleotide sequence similarity ranged from 47-100% in the 5' NTR and from 63-100% in the P1 coding region. The lowest amino acid similarity between sequences of P1 gene was 55%. In phylogenetic trees of 5'NTR analysis, two PVY(O) Mexican isolates clustered with other PVY(O) isolates. In turn, the three PVY(N) isolates grouped with PVY(N-NTN) isolates. The phylogenetic analysis of P1 sequences (nucleotide and amino acid) showed two PVY(O) isolates grouping next to N-NTN cluster. A detailed analysis of the PVY(O) isolates showed two potential recombination regions in the P1 gene, in contrast to 5'NTR where no recombination was detected.
Ramírez-Rodríguez
Victoriano Roberto
VR
Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional, Campus Guanajuato, Guanajuato, Mexico. vicr358@yahoo.com
Frías-Treviño
Gustavo
G
Silva-Rosales
Laura
L
Aviña-Padilla
Katia
K
Martínez-Soriano
Juan Pablo
JP
D020121:5' Untranslated Regions; D016000:Cluster Analysis; D008800:Mexico; D008969:Molecular Sequence Data; D010802:Phylogeny; D010935:Plant Diseases / Q000821:virology; D017800:Potyvirus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D012367:RNA, Viral / Q000235:genetics; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D012703:Serotyping; D011198:Solanum tuberosum / Q000821:virology*; D014026:Nicotiana / Q000821:virology; D014764:Viral Proteins / Q000235:genetics
D020121:5' Untranslated Regions; D012367:RNA, Viral; D014764:Viral Proteins