Molecular analysis of surface glycoprotein multigene family TrGP expressed on the plasma membrane of Trypanosoma rangeli epimastigotes forms.
Trypanosoma rangeli, a non-pathogenic hemoflagelate that in Central and South America infects humans, shares with Trypanosoma cruzi reservoirs and triatomine vectors, as well as geographical distribution. Recently, we have described in T. rangeli a truncated gene copy belonging to the group II of the trans-sialidase superfamily (TrGP). This superfamily, collectively known in T. cruzi as gp85/TS, includes members that are involved in host cell invasion and infectivity. To confirm the presence of this superfamily in the genome of T. rangeli and obtain a better knowledge of its characteristics, we designed a PCR and RT-PCR cloning strategy to allow sequence analysis of both genomic and transcribed copies. We identified two full-length copies of TrGP, some pseudogenes, and N- and C-terminal sequences of several genes. We also analyzed the expression and cellular localization of these proteins in epimastigote forms of a Venezuelan T. rangeli isolate using polyclonal antibodies made against a recombinant peptide from the N-terminal region of a TrGP member. We confirmed that TrGP is a multigenic family that shares many features with T. cruzi gp85/TS, including the telomeric location of some of its members, and by immunofluorescence analysis that its location is at the surface of the parasite.
Laboratorio de Genética Molecular Dr. Yunis-Turbay, Decanato de Ciencias de la Salud, Universidad Centroccidental Lisandro Alvarado, Barquisimeto, Venezuela.
C P
CP
Peña
N
N
Lander
E
E
Rodríguez
G
G
Crisante
N
N
Añez
J L
JL
Ramírez
M A
MA
Chiurillo
D000818:Animals; D002462:Cell Membrane / Q000737:chemistry; D016054:DNA, Protozoan / Q000235:genetics; D020869:Gene Expression Profiling; D006023:Glycoproteins / Q000032:analysis / Q000235:genetics*; D006801:Humans; D008562:Membrane Glycoproteins / Q000032:analysis / Q000235:genetics*; D005810:Multigene Family; D009439:Neuraminidase / Q000032:analysis / Q000235:genetics*; D010750:Phosphoproteins / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods; D015800:Protozoan Proteins / Q000032:analysis / Q000235:genetics*; D014345:Trypanosoma / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification; D014687:Venezuela
D016054:DNA, Protozoan; C038790:GP72 antigen, Trypanosoma; D006023:Glycoproteins; D008562:Membrane Glycoproteins; D010750:Phosphoproteins; D015800:Protozoan Proteins; C069715:trans-sialidase; D009439:Neuraminidase