AtGRP5, a vacuole-located glycine-rich protein involved in cell elongation.
Although several glycine-rich protein (GRP) genes were isolated and characterized, very little is known about their function. The primary structure of AtGRP5 from Arabidopsis thaliana has a signal peptide followed by a region with high glycine content. In this work, green fluorescent protein fusions were obtained in order to characterize the sub-cellular localization of the AtGRP5 protein. The results indicated that this protein is the first described vacuolar GRP. Sense, antisense and RNAi transgenic A. thaliana plants were generated and analyzed phenotypically. Plants overexpressing AtGRP5 showed longer roots and an enhanced elongation of the inflorescence axis, while antisense and RNAi plants demonstrated the opposite phenotype. The analysis of a knockout T-DNA line corroborates the phenotypes obtained with the antisense and RNAi plants. Altogether, these results suggest that this vacuolar GRP could be involved in organ growth by promoting cell elongation processes.
Laboratório de Genômica Funcional e Transdução de Sinal, Departamento de Genética, Universidade Federal do Rio de Janeiro, Rio de Janeiro, C.P. 68011, 21941-970, Brazil.
Amanda
A
Mangeon
Claudia
C
Magioli
Adriana Dias
AD
Menezes-Salgueiro
Vanessa
V
Cardeal
Cristina
C
de Oliveira
Vinícius Costa
VC
Galvão
Rogério
R
Margis
Gilbert
G
Engler
Gilberto
G
Sachetto-Martins
D017360:Arabidopsis / Q000166:cytology / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000502:physiology*; D048430:Cell Shape / Q000235:genetics / Q000502:physiology*; D004269:DNA, Bacterial / Q000235:genetics; D018506:Gene Expression Regulation, Plant / Q000235:genetics / Q000502:physiology; D005998:Glycine / Q000737:chemistry*; D018613:Microscopy, Confocal; D030821:Plants, Genetically Modified / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014617:Vacuoles / Q000378:metabolism*
D029681:Arabidopsis Proteins; D004269:DNA, Bacterial; C036483:T-DNA; D005998:Glycine