Acetylcholinesterase biosensor based on gold nanoparticles and cysteamine self assembled monolayer for determination of monocrotophos.
In this paper, a simple method for immobilization of acetylcholinesterase (AChE) on cysteamine assembled glassy carbon electrode coupled with gold nanoparticles (GNPs) was proposed and thus a sensitive, fast and stable amperometric biosensor for quantitative determination of monocrotophos was developed. The fabrication procedure was characterized by cyclic voltammetry, electrochemical impedance spectroscopy and contact angles. The presence of GNPs not only led to an increased effective surface to provide a sufficient amount of sites for binding enzyme, but also promoted electron transfer reactions and catalyzed the electro-oxidation of thiocholine, thus amplifying the detection sensitivity. Due to the notable decrease in voltammetric signal of the immobilized AChE, a simple method for determination of monocrotophos was established. The inhibition of monocrotophos was proportional to its concentration in two ranges, from 0.5 to 10 ng mL(-1) and from 10 to 600 ng mL(-1), with a detection limit lower than 0.3 ng mL(-1). The constructed biosensor processing prominent characteristics and performance such as good precision and reproducibility, acceptable stability and accuracy, fast response and low detection limit has potential application in detection of toxic compounds.
Key Laboratory of Pesticide and Chemical Biology of Ministry of Education, College of Chemistry, Central China Normal University, Wuhan 430079, P. R. China.
Dan
D
Du
Wenjuan
W
Chen
Jie
J
Cai
Jianming
J
Zhang
Haiyang
H
Tu
Aidong
A
Zhang
D000110:Acetylcholinesterase / Q000737:chemistry* / Q000378:metabolism*; D015374:Biosensing Techniques / Q000379:methods*; D002800:Cholinesterase Inhibitors / Q000032:analysis / Q000378:metabolism; D004800:Enzymes, Immobilized / Q000737:chemistry / Q000378:metabolism; D006046:Gold / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry*; D008999:Monocrotophos / Q000032:analysis* / Q000378:metabolism; D012680:Sensitivity and Specificity
D002800:Cholinesterase Inhibitors; D004800:Enzymes, Immobilized; D008999:Monocrotophos; D006046:Gold; D000110:Acetylcholinesterase