Control of Listeria monocytogenes on frankfurters by dipping in hops beta acids solutions.
Hops beta acids (HBA) are parts of hops flowers used in beer brewing and have shown antilisterial activity in bacteriological broth. The U.S. Department of Agriculture, Food Safety and Inspection Service has approved HBA for use to control Listeria monocytogenes on ready-to-eat meat products. This study evaluated the effects of HBA as dipping solutions to control L. monocytogenes during storage of frankfurters. Frankfurters (two replicates and three samples each) were inoculated (1.9 +/- 0.1 log CFU/cm2) with L. monocytogenes (10-strain mixture), dipped (2 min, 25 +/- 2 degrees C) in HBA solutions (0.03, 0.06, and 0.10%) or distilled water, and then vacuum packaged and stored at 4 or 10 degrees C for up to 90 and 48 days, respectively. Samples were periodically analyzed for microbial survival and growth on tryptic soy agar plus 0.6% yeast extract and PALCAM agar. Dipping in HBA solutions caused immediate L. monocytogenes reductions (P < 0.05) of 1.3 to 1.6 log CFU/cm2, whereas distilled water reduced counts by 1.0 log CFU/cm2. Pathogen growth was completely suppressed (P < 0.05) for 30 to 50 (4 degrees C) or 20 to 28 (10 degrees C) days on frankfurters dipped in HBA solutions, with antilisterial effects increasing with higher concentrations (0.03 to 0.10%). Fitting the data with the Baranyi model confirmed that the lag-phase duration of the pathogen was extended, and the growth rate was decreased on samples dipped in HBA solutions. Therefore, HBA may be considered for use to improve the microbial safety of ready-to-eat meat products, provided that future studies show no adverse effects on sensory qualities and that their use is economically feasible.
Center for Meat Safety and Quality and Food Safety Cluster, Department of Animal Sciences, Colorado State University, Fort Collins, Colorado 80523, USA.
Cangliang
C
Shen
Ifigenia
I
Geornaras
Patricia A
PA
Kendall
John N
JN
Sofos
D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology*; D003296:Cooking; D005511:Food Handling / Q000379:methods*; D005516:Food Microbiology; D005519:Food Preservation; D027582:Humulus / Q000737:chemistry*; D008089:Listeria monocytogenes / Q000187:drug effects*; D008461:Meat Products / Q000382:microbiology*; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*
D000900:Anti-Bacterial Agents; D010936:Plant Extracts