Immobilization of hemoglobin at the galleries of layered lepidocrocite-related potassium lithium titanate.
The exfoliated nanosheets from the layered potassium lithium titanate K0.8Ti1.73Li0.27O4 can be assembled with the hemoglobin through the electrostatic attraction under mild conditions. The immobilized enzyme has regularly layered structure based on the small angel X-ray diffraction. The preparation method is low cost and the maximum bounding capacity of enzyme is large. The enzyme activity study showed that the immobilized Hb retained enzyme-like peroxidase activity, and the activity increased along with the increase of the interlayer distances. Michaelis-Menten model methods were used to study the peroxidase activity.
State Key Laboratory of High Performance Ceramics and Superfine Microstructures, Graduate School, Shanghai Institute of Ceramics, Chinese Academy of Sciences, Shanghai, China.
Yihua
Y
Wu
Qiuming
Q
Gao
Lu
L
Gao
D005290:Ferric Compounds / Q000737:chemistry*; D006454:Hemoglobins / Q000737:chemistry*; D008094:Lithium / Q000737:chemistry*; D011188:Potassium / Q000737:chemistry*; D014025:Titanium / Q000737:chemistry*; D014961:X-Ray Diffraction
D005290:Ferric Compounds; D006454:Hemoglobins; C540954:potassium lithium titanate; C000499:ferric oxide; D008094:Lithium; D014025:Titanium; D011188:Potassium