Engineering an artificial amoeba propelled by nanoparticle-triggered actin polymerization.
We have engineered an amoeba system combining nanofabricated inorganic materials with biological components, capable of propelling itself via actin polymerization. The nanofabricated materials have a mechanism similar to the locomotion of the Listeria monocytogenes, food poisoning bacteria. The propulsive force generation utilizes nanoparticles made from nickel and gold functionalized with the Listeria monocytogenes transmembrane protein, ActA. These Listeria-mimic nanoparticles were in concert with actin, actin binding proteins, ATP (adenosine triphosphate) and encapsulated within a lipid vesicle. This system is an artificial cell, such as a vesicle, where artificial nanobacteria and actin polymerization machinery are used in driving force generators inside the cell. The assembled structure was observed to crawl on a glass surface analogously to an amoeba, with the speed of the movement dependent on the amount of actin monomers and ATP present.
Yi
Jinsoo
J
Department of Bioengineering, University of California Los Angeles, 420 Westwood Plaza, 7523 Boelter Hall, Los Angeles, CA 90095-1600, USA.
Schmidt
Jacob
J
Chien
Aichi
A
Montemagno
Carlo D
CD
D000199:Actins / Q000737:chemistry* / Q000648:ultrastructure; D000656:Amoeba / Q000737:chemistry* / Q000502:physiology; D000818:Animals; D001426:Bacterial Proteins / Q000737:chemistry* / Q000648:ultrastructure; D032701:Biomimetics / Q000379:methods*; D019281:Dimerization; D008124:Locomotion; D008565:Membrane Proteins / Q000737:chemistry* / Q000648:ultrastructure; D009038:Motion; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D036103:Nanotechnology / Q000379:methods*; D011108:Polymers / Q000737:chemistry
D000199:Actins; D001426:Bacterial Proteins; D008565:Membrane Proteins; D011108:Polymers; C072980:actA protein, Listeria monocytogenes