Targeted deletion of the gamma-adducin gene (Add3) in mice reveals differences in alpha-adducin interactions in erythroid and nonerythroid cells.
In red blood cells (RBCs) adducin heterotetramers localize to the spectrin-actin junction of the peripheral membrane skeleton. We previously reported that deletion of beta-adducin results in osmotically fragile, microcytic RBCs and a phenotype of hereditary spherocytosis (HS). Notably, alpha-adducin was significantly reduced, while gamma-adducin, normally present in limited amounts, was increased approximately 5-fold, suggesting that alpha-adducin requires a heterologous binding partner for stability and function, and that gamma-adducin can partially substitute for the absence of beta-adducin. To test these assumptions we generated gamma-adducin null mice. gamma-adducin null RBCs appear normal on Wright's stained peripheral blood smears and by scanning electron microscopy. All membrane skeleton proteins examined are present in normal amounts, and all hematological parameters measured are normal. Despite a loss of approximately 70% of alpha-adducin in gamma-adducin null platelets, no bleeding defect is observed and platelet structure appears normal. Moreover, systemic blood pressure and pulse are normal in gamma-adducin null mice. gamma- and beta-adducin null mice were intercrossed to generate double null mice. Loss of gamma-adducin does not exacerbate the beta-adducin null HS phenotype although the amount alpha-adducin is reduced to barely detectable levels. The stability of alpha-adducin in the absence of a heterologous binding partner varies considerably in various tissues. The amount of alpha-adducin is modestly reduced ( approximately 15%) in the kidney, while in the spleen and brain is reduced by approximately 50% with the loss of a heterologous beta- or gamma-adducin binding partner. These results suggest that the structural properties of adducin differ significantly between erythroid and various nonerythroid cell types.
The Jackson Laboratory, Bar Harbor, Maine 04606, USA.
Kenneth E
KE
Sahr
Amy J
AJ
Lambert
Steven L
SL
Ciciotte
Narla
N
Mohandas
Luanne L
LL
Peters
D000818:Animals; D001792:Blood Platelets / Q000166:cytology / Q000378:metabolism / Q000502:physiology*; D015152:Blotting, Northern; D015153:Blotting, Western; D001921:Brain / Q000166:cytology; D002148:Calmodulin-Binding Proteins / Q000097:blood / Q000172:deficiency* / Q000235:genetics; D003598:Cytoskeletal Proteins / Q000097:blood / Q000172:deficiency / Q000235:genetics; D004910:Erythrocyte Membrane / Q000378:metabolism / Q000502:physiology; D004912:Erythrocytes / Q000166:cytology / Q000378:metabolism / Q000502:physiology*; D005260:Female; D017353:Gene Deletion; D007668:Kidney / Q000166:cytology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D008855:Microscopy, Electron, Scanning; D009996:Osmotic Fragility; D013154:Spleen / Q000166:cytology; D000099291:Adducins
D002148:Calmodulin-Binding Proteins; D003598:Cytoskeletal Proteins; D000099291:Adducins