Molecularly imprinted polymers for simultaneous determination of antiepileptics in river water samples by liquid chromatography-tandem mass spectrometry.
A restricted access media-molecularly imprinted polymer (RAM-MIP) for cyclobarbital has been developed for selective extraction of antiepileptics in river water samples. The RAM-MIP was prepared using 4-vinylpyridine and ethylene glycol dimethacrylate as a functional monomer and cross-linker, respectively, by a multi-step swelling and polymerization method followed by a surface modification technique. The RAM-MIP for cyclobarbital showed molecular recognition abilities for phenobarbital, amobarbital and phenytoin as well as cyclobarbital. Thus, selective analysis of antiepileptics in river water samples was attained with RAM-MIP extraction followed by column-switching liquid chromatography-tandem mass spectrometry. The concentrations of phenobarbital and phenytoin in river water samples were about 15 and 4 ng/L, respectively, while that of amobarbital was below the limit of quantitation.
Faculty of Pharmaceutical Sciences, Mukogawa Women's University, Nishinomiya, Hyogo, Japan.
Kaori
K
Hoshina
Shizuyo
S
Horiyama
Hisami
H
Matsunaga
Jun
J
Haginaka
D000927:Anticonvulsants / Q000032:analysis*; D001463:Barbiturates / Q000032:analysis; D002853:Chromatography, Liquid; D004350:Drug Residues / Q000032:analysis*; D006863:Hydrogen-Ion Concentration; D054802:Molecular Imprinting; D010672:Phenytoin / Q000032:analysis; D015203:Reproducibility of Results; D045483:Rivers / Q000737:chemistry*; D012680:Sensitivity and Specificity; D053719:Tandem Mass Spectrometry; D014874:Water Pollutants, Chemical / Q000032:analysis*
D000927:Anticonvulsants; D001463:Barbiturates; D014874:Water Pollutants, Chemical; D010672:Phenytoin