title: [Measurement of airway resistance and reactivity in guinea pigs using double-chamber plethysmography].
abstract: OBJECTIVE
  To establish a method for measurement of airway resistance (sRaw) and reactivity in guinea pigs.
  
  
  METHODS
  Methacholine spray at gradient concentrations was given to guinea pigs. PC100 was defined as the concentration of methacholine when the sRaw doubled in the guinea pigs using a double-chamber plethysmograph. The time for the recovery of PC100 resistance to baseline levels was measured. The sRaw and PC100 were measured twice on days 1 and 15 (4 time points) in the guinea pigs before and after OVA challenge.
  
  
  RESULTS
  PC100 in a normal guinea pig airway was shown to recover the baseline level within 1 h. Double-chamber plethysmographical measurement of the sRaw and PC100 in normal guinea pigs did not show significant differences between the time points [sRaw: 3.25-/+0.67, 3.33-/+0.58, 3.30-/+0.56, and 3.32-/+0.75 cm H2O.s; log2PC100: 8.48-/+0.94, 8.64-/+1.04, 8.56-/+0.67, and 8.64-/+0.60, respectively, P>0.05]. The sRaw and airway reactivity were significantly increased in guinea pigs challenged with OVA [sRaw: 7.08-/+1.82 vs 2.87-/+0.53 cmH2O.s, P<0.01; log2PC100: 6.64-/+1.26 vs 8.48-/+1.17, P<0.01].
  
  
  CONCLUSIONS
  A double-chamber plethysmography for measurement of sRaw and airway reactivity in guinea pig is established successfully.
authors.affiliation: State Key Laboratory of Respiratory Disease, Guangzhou Medical College, First Affiliated Hospital, Guangzhou 510120, China. yao7722@ yahoo.com.cn
authors.forename: Wei-min
authors.initials: WM
authors.lastname: Yao
authors.forename: Ke-fang
authors.initials: KF
authors.lastname: Lai
authors.forename: Yuan-ming
authors.initials: YM
authors.lastname: Luo
authors.forename: Chun-li
authors.initials: CL
authors.lastname: Liu
authors.forename: Ru-chong
authors.initials: RC
authors.lastname: Chen
authors.forename: Wei
authors.initials: W
authors.lastname: Luo
authors.forename: Nan-shan
authors.initials: NS
authors.lastname: Zhong
mesh_terms: D000403:Airway Resistance; D000818:Animals; D001249:Asthma / Q000139:chemically induced / Q000503:physiopathology; D016535:Bronchial Hyperreactivity / Q000209:etiology / Q000503:physiopathology*; D006168:Guinea Pigs; D008297:Male; D016210:Methacholine Chloride; D010991:Plethysmography / Q000295:instrumentation / Q000379:methods*; D011897:Random Allocation
chemical_list: D016210:Methacholine Chloride