title: Hydrogen production by a hyperthermophilic membrane-bound hydrogenase in water-soluble nanolipoprotein particles.
abstract: Hydrogenases constitute a promising class of enzymes for ex vivo hydrogen production. Implementation of such applications is currently hindered by oxygen sensitivity and, in the case of membrane-bound hydrogenases (MBHs), poor water solubility. Nanolipoprotein particles (NLPs) formed from apolipoproteins and phospholipids offer a novel means of incorporating MBHs into a well-defined water-soluble matrix that maintains the enzymatic activity and is amenable to incorporation into more complex architectures. We report the synthesis, hydrogen-evolving activity, and physical characterization of the first MBH-NLP assembly. This may ultimately lead to the development of biomimetic hydrogen-production devices.
authors.affiliation: Physical and Life Sciences Directorate, Lawrence Livermore National Laboratory, USA.
authors.forename: Sarah E
authors.initials: SE
authors.lastname: Baker
authors.forename: Robert C
authors.initials: RC
authors.lastname: Hopkins
authors.forename: Craig D
authors.initials: CD
authors.lastname: Blanchette
authors.forename: Vicki L
authors.initials: VL
authors.lastname: Walsworth
authors.forename: Rhoda
authors.initials: R
authors.lastname: Sumbad
authors.forename: Nicholas O
authors.initials: NO
authors.lastname: Fischer
authors.forename: Edward A
authors.initials: EA
authors.lastname: Kuhn
authors.forename: Matt
authors.initials: M
authors.lastname: Coleman
authors.forename: Brett A
authors.initials: BA
authors.lastname: Chromy
authors.forename: Sonia E
authors.initials: SE
authors.lastname: Létant
authors.forename: Paul D
authors.initials: PD
authors.lastname: Hoeprich
authors.forename: Michael W W
authors.initials: MW
authors.lastname: Adams
authors.forename: Paul T
authors.initials: PT
authors.lastname: Henderson
mesh_terms: D001053:Apolipoproteins / Q000737:chemistry*; D002462:Cell Membrane / Q000201:enzymology; D004800:Enzymes, Immobilized / Q000737:chemistry*; D006859:Hydrogen / Q000737:chemistry*; D006864:Hydrogenase / Q000737:chemistry*; D053758:Nanoparticles / Q000737:chemistry*; D010743:Phospholipids / Q000737:chemistry*; D020027:Pyrococcus furiosus / Q000201:enzymology; D012995:Solubility; D014867:Water / Q000737:chemistry
chemical_list: D001053:Apolipoproteins; D004800:Enzymes, Immobilized; D010743:Phospholipids; D014867:Water; D006859:Hydrogen; D006864:Hydrogenase