pGreen-S: a clone vector bearing absence of enhanced green fluorescent protein for screening recombinants.
The bacterial cloning vector, pGreen-S, was constructed by inserting the enhanced green fluorescent protein (EGFP) gene at the XbaI restriction site of pUC18 plasmid. When expressed in Escherichia coli DH5alpha produced colonies that were an absinthe green color under daylight and strongly fluorescent green under longwave ultraviolet light. The pGreen-S vector was used to select for directional insert based on the loss of green fluorescence in recombinant colonies that was caused by the absence of EGFP. The EGFP reporter system differs from the conventional complementation of lacZ, making screening recombinants simpler, less expensive, and more effective.
Tang
Jinbao
J
Department of Basic Medicine, Weifang Medical University, Weifang, Shandong 261042, China. tangjinbao@yahoo.com.cn
Liang
Shujuan
S
Zhang
Jinbao
J
Gao
Zhiqin
Z
Zhang
Suhua
S
D004926:Escherichia coli / Q000378:metabolism; D005822:Genetic Vectors; D049452:Green Fluorescent Proteins / Q000235:genetics* / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D014466:Ultraviolet Rays
D011993:Recombinant Fusion Proteins; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins