Salidroside, the main active compound of Rhodiola plants, inhibits high glucose-induced mesangial cell proliferation.
Because Rhodiola plants are known to have a protective effect on diabetic nephropathy, this study aimed to investigate the inhibitory effect of salidroside, the main active component of Rhodiola plants, on high glucose-induced mesangial cell proliferation and its possible mechanism. Salidroside (1 approximately 100 microM) dose dependently inhibited high glucose-induced mesangial cell early proliferation. Exposure of mesangial cells to high glucose for 24 h significantly induced reactive oxygen species (ROS) accumulation, ERK1/2 phosphorylation, and p27 (Kip1) expression, and these changes were dramatically inhibited by salidroside in a dose-dependent manner. High glucose-promoted TGF- beta1 secretion was also significantly attenuated by treatment of mesangial cells with salidroside. These results indicated that salidroside had the ability to inhibit high glucose-induced mesangial cell proliferation, which is in correlation with salidroside suppressing TGF- beta1 production and ERK1/2 phosphorylation.
Yin
Dengke
D
School of Life Science and Technology, China Pharmaceutical University, Nanjing, P. R. China.
Yao
Wenbing
W
Chen
Song
S
Hu
Rongfeng
R
Gao
Xiangdong
X
D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects*; D005947:Glucose / Q000008:administration & dosage / Q000494:pharmacology*; D005960:Glucosides / Q000737:chemistry / Q000494:pharmacology*; D006801:Humans; D050527:Mesangial Cells / Q000166:cytology / Q000187:drug effects*; D019950:Mitogen-Activated Protein Kinase 1 / Q000378:metabolism; D048052:Mitogen-Activated Protein Kinase 3 / Q000378:metabolism; D010636:Phenols / Q000737:chemistry / Q000494:pharmacology*; D010766:Phosphorylation; D029771:Rhodiola / Q000737:chemistry*; D053773:Transforming Growth Factor beta1 / Q000378:metabolism
D005960:Glucosides; D010636:Phenols; D053773:Transforming Growth Factor beta1; D019950:Mitogen-Activated Protein Kinase 1; D048052:Mitogen-Activated Protein Kinase 3; D005947:Glucose; C009172:rhodioloside