title: [Experimental study on effect of total flavone of Ginkgo biloba on delaying cell senescence].
abstract: OBJECTIVE
  To investigate the mechanism of delaying the senescence of human diploid fibroblast (2BS) by total flavone of Ginkgo biloba (FG).
  
  
  METHODS
  The drug sera of FG was used to treat the 2BS. The population doublings of 2BS cells were observed, the mRNA expression of P16 gene was determined by fluorescence real-time quantitative PCR.
  
  
  RESULTS
  FG significantly extended the population doublings of 2BS cells, and decreased the expression of P16 mRNA.
  
  
  CONCLUSIONS
  FG can delay the senescence of cells by inhibiting the P16 gene expression.
authors.affiliation: Department of Biology, Anhui Medical College, Hefei 230061, China. sxp@ahyz.cn
authors.forename: Xiao-ping
authors.initials: XP
authors.lastname: Song
authors.forename: Zhi-wu
authors.initials: ZW
authors.lastname: Chen
authors.forename: An-ning
authors.initials: AN
authors.lastname: Fang
authors.forename: Pei-fei
authors.initials: PF
authors.lastname: Fang
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D002453:Cell Cycle / Q000187:drug effects; D002478:Cells, Cultured; D016922:Cellular Senescence / Q000187:drug effects*; D019941:Cyclin-Dependent Kinase Inhibitor p16 / Q000378:metabolism*; D047309:Flavones / Q000494:pharmacology*; D005786:Gene Expression Regulation; D020441:Ginkgo biloba / Q000737:chemistry*; D006801:Humans; D008297:Male; D018515:Plant Leaves / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats
chemical_list: D000975:Antioxidants; D019941:Cyclin-Dependent Kinase Inhibitor p16; D047309:Flavones; D012333:RNA, Messenger