title: Biocompatibility evaluation of chitosan-based injectable hydrogels for the culturing mice mesenchymal stem cells in vitro.
abstract: A thermosensitive polymer can be held liquid before being injected to deliver living cells or therapeutic agents and formed monolithic gels when injected in vivo. In this study, chitosan-based thermosensitive hydrogels were prepared, characterized and the biocompatibility for culturing mice bone mesenchymal stem cells (BMSC) on 2D films and within 3D hydrogel were investigated. The gelation temperature and biocompatibility could be modulated by addition hydroxyethyl cellulose (HEC) to chitosan-glycerophosphate (CH-GP) formulation. The CH-GP-HEC liquid solution can turn into gel at body temperature and has highly compatible with BMSC. Therefore, the CH-GP-HEC gel could be used as an attractive injected in-situ forming scaffold for future applications of delivering biologically active therapeutics for tissue engineering filed.
authors.affiliation: School of Basic Medical Science, Capital Medical University, Beijing 100069, China.
authors.forename: Jihong
authors.initials: J
authors.lastname: Yan
authors.forename: Liu
authors.initials: L
authors.lastname: Yang
authors.forename: Guirong
authors.initials: G
authors.lastname: Wang
authors.forename: Yang
authors.initials: Y
authors.lastname: Xiao
authors.forename: Baohong
authors.initials: B
authors.lastname: Zhang
authors.forename: Nianmin
authors.initials: N
authors.lastname: Qi
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D001842:Bone and Bones / Q000166:cytology; D018929:Cell Culture Techniques / Q000379:methods*; D019070:Cell Lineage; D002482:Cellulose / Q000031:analogs & derivatives / Q000737:chemistry; D048271:Chitosan / Q000737:chemistry*; D005994:Glycerophosphates / Q000737:chemistry; D020100:Hydrogels / Q000737:chemistry*; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D051379:Mice; D051381:Rats; D017207:Rats, Sprague-Dawley; D012212:Rheology; D023822:Tissue Engineering / Q000379:methods
chemical_list: D001672:Biocompatible Materials; D005994:Glycerophosphates; D020100:Hydrogels; D002482:Cellulose; C002283:hydroxyethylcellulose; D048271:Chitosan