title: Developing genomic platforms for Chinese hamster ovary cells.
abstract: Chinese hamster ovary (CHO) cells are widely used in recombinant protein production, yet despite their importance in bioprocessing, few genomic resources have been developed for this cell line. Over the past several years, we have made considerable progress in the development of genomic tools for CHO. Using Sanger-based sequencing technology, we have accrued a sequence repertoire of more than 68,000 expressed sequence tags (ESTs), representing more than 28,000 unique CHO transcripts. Using closely related species, we have functionally annotated this sequence set and have currently achieved significant representation in a number of functional classes, including some closely tied to recombinant protein production. This sequence repository has been used to design custom CHO Affymetrix arrays for transcriptome analysis. Illumina Solexa deep sequencing technology was also applied to study the CHO cell transcriptome and survey the identity and expression of small RNAs. These applications demonstrate the utility of genomic tools, and illustrate the applicability of emerging next-generation sequencing technologies.
authors.affiliation: Department of Chemical Engineering and Materials Science, University of Minnesota, 421 Washington Avenue SE, Minneapolis, MN 55455-0132 USA.
authors.forename: Anne
authors.initials: A
authors.lastname: Kantardjieff
authors.affiliation: Bioprocessing Technology Institute Agency for Science, Technology and Research (A⁎STAR) 20 Biopolis Way, #06-01 Centros, 138668, Singapore.
authors.forename: Peter Morin
authors.initials: PM
authors.lastname: Nissom
authors.affiliation: Bioprocessing Technology Institute Agency for Science, Technology and Research (A⁎STAR) 20 Biopolis Way, #06-01 Centros, 138668, Singapore.
authors.forename: Song Hui
authors.initials: SH
authors.lastname: Chuah
authors.affiliation: Bioprocessing Technology Institute Agency for Science, Technology and Research (A⁎STAR) 20 Biopolis Way, #06-01 Centros, 138668, Singapore.
authors.forename: Faraaz
authors.initials: F
authors.lastname: Yusufi
authors.affiliation: Department of Chemical Engineering and Materials Science, University of Minnesota, 421 Washington Avenue SE, Minneapolis, MN 55455-0132 USA.
authors.forename: Nitya M
authors.initials: NM
authors.lastname: Jacob
authors.affiliation: Department of Chemical Engineering and Materials Science, University of Minnesota, 421 Washington Avenue SE, Minneapolis, MN 55455-0132 USA.
authors.forename: Bhanu Chandra
authors.initials: BC
authors.lastname: Mulukutla
authors.affiliation: Bioprocessing Technology Institute Agency for Science, Technology and Research (A⁎STAR) 20 Biopolis Way, #06-01 Centros, 138668, Singapore.
authors.forename: Miranda
authors.initials: M
authors.lastname: Yap
authors.affiliation: Department of Chemical Engineering and Materials Science, University of Minnesota, 421 Washington Avenue SE, Minneapolis, MN 55455-0132 USA. Electronic address: acre@cems.umn.edu.
authors.forename: Wei-Shou
authors.initials: WS
authors.lastname: Hu
mesh_terms: D000818:Animals; D016466:CHO Cells; D006224:Cricetinae; D003412:Cricetulus; D023281:Genomics; D035683:MicroRNAs / Q000235:genetics
chemical_list: D035683:MicroRNAs