Liquid chromatography mass spectrometry for the determination of salvianolic acid B, a natural compound from the herb Danshen in rat plasma and application to pharmacokinetic study.
A sensitive and specific liquid chromatographic-electrospray ionization mass spectrometric method was developed for quantification of salvianolic acid B in rat plasma with resveratrol as the internal standard. The analytes were separated on a reversed-phase column with acetonitrile (40%) and water (60%) containing 0.75% formic acid as mobile phase at a flow rate of 1 mL/min. Liquid-liquid extraction was adopted for the sample preparation, and the analytes were determined using electrospray negative ionization mass spectrometry in the selective monitoring mode. The method was validated over the concentration range 0.1-40 microg/mL using 0.1 mL of plasma with coefficients of correlation >0.999. The intra- and inter-day precisions of analysis were <10%, and accuracy ranged from 94 to 101%. This method was successfully applied to a pharmacokinetics of salvianolic acid B in rats.
Han
De-En
DE
Center of Drug Metabolism and Pharmacokinetics, China Pharmaceutical University 24 Tongjiaxiang, Nanjing 210009, Jiangsu, People's Republic of China.
Gao
Zi-Dong
ZD
Zhao
Di
D
Wang
Lei
L
Li
Na
N
Li
Ting-Ting
TT
Wu
Lei
L
Chen
Xi-Jing
XJ
D000818:Animals; D019540:Area Under Curve; D001572:Benzofurans / Q000097:blood* / Q000493:pharmacokinetics; D002853:Chromatography, Liquid / Q000379:methods*; D004355:Drug Stability; D016018:Least-Squares Analysis; D008297:Male; D018517:Plant Roots / Q000737:chemistry; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D027561:Salvia miltiorrhiza / Q000737:chemistry*; D012680:Sensitivity and Specificity; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D013696:Temperature; D053630:Depsides
D001572:Benzofurans; C076944:salvianolic acid B; D053630:Depsides