title: The effect of pH, bile and calcium on the adhesion ability of probiotic enterococci of animal origin to the porcine jejunal epithelial cell line IPEC-J2.
abstract: Examination of adhesion ability using a quantitative assay based on radiolabelled bacteria showed that 10 Enterococcus strains exhibited adhesion ability from 2 to 4%. Enterococcus faecium EF2019 (isolate from rabbit faeces, deponed to Czech Culture of Microorganisms in Brno, CCM 7420) showed the highest adhesion ability (4.0+/-0.4%). With regard to survival, all strains displayed good resistance towards 0.3% oxgall and HCl (pH 3.0). Pretreatment of strains with HCl (pH 3.0) significantly reduced their adhesion. Pretreatment of strains by oxgall significantly reduced the adhesion capacity of E. faecium EF2019, EF1839 and EF319 strains, while the adhesion ability of E. faecium EE3 (isolate from canine feed) slightly increased. Furthermore, addition of calcium (200 mmol/l) significantly increased (P<0.001) the adhesion ability for all strains tested. The adhesion ability of the isolates from rabbits, EF1839 and EF529, as well as the isolate EE3 (strain from canine feed) increased from 2-3% up to 50-55% upon calcium addition. Despite, in general low adhesive properties, strains can survive passage through the gastrointestinal tract.
authors.affiliation: Institute of Animal Physiology, Slovak Academy of Sciences, Soltésovej 4-6, 04001 Kosice, Slovakia. mmarcinakova@nextra.sk
authors.forename: Miroslava
authors.initials: M
authors.lastname: Marcináková
authors.forename: Trine Danø
authors.initials: TD
authors.lastname: Klingberg
authors.forename: Andrea
authors.initials: A
authors.lastname: Lauková
authors.forename: Birgitte Bjørn
authors.initials: BB
authors.lastname: Budde
mesh_terms: D000143:Acids / Q000633:toxicity*; D000818:Animals; D001422:Bacterial Adhesion / Q000187:drug effects*; D001646:Bile; D002118:Calcium / Q000633:toxicity*; D003902:Detergents / Q000633:toxicity*; D016983:Enterococcus / Q000187:drug effects*; D004847:Epithelial Cells / Q000382:microbiology*; D019936:Probiotics
chemical_list: D000143:Acids; D003902:Detergents; D002118:Calcium