title: Comparison of the Ames II and traditional Ames test responses with respect to mutagenicity, strain specificities, need for metabolism and correlation with rodent carcinogenicity.
abstract: The Ames II Salmonella mutagenicity assay procedure was used to test 71 chemicals, and the results were compared with those from the traditional Ames Salmonella test using the NTP database as the reference. All Ames II tests were performed using a fluctuation procedure in microplate format, using TAMix for the detection of base pair substitutions and TA98 to detect frameshift mutations. There was 84% agreement between the two procedures in identifying mutagens and non-mutagens, which is equivalent to the intra- and interlaboratory reproducibility of 87% for the traditional test. The two tests also performed similarly in their predictions of rodent carcinogenicity.
authors.affiliation: Xenometrix AG, Allschwil, Switzerland. mk@xenometrix.ch
authors.forename: Markus
authors.initials: M
authors.lastname: Kamber
authors.forename: Sini
authors.initials: S
authors.lastname: Flückiger-Isler
authors.forename: Günter
authors.initials: G
authors.lastname: Engelhardt
authors.forename: Rudolf
authors.initials: R
authors.lastname: Jaeckh
authors.forename: Errol
authors.initials: E
authors.lastname: Zeiger
mesh_terms: D000818:Animals; D002273:Carcinogens / Q000633:toxicity*; D020111:Chlorodiphenyl (54% Chlorine) / Q000633:toxicity; D006224:Cricetinae; D009152:Mutagenicity Tests / Q000379:methods*; D009153:Mutagens / Q000633:toxicity*; D051381:Rats; D015203:Reproducibility of Results; D012475:Salmonella / Q000378:metabolism*; D013045:Species Specificity
chemical_list: D002273:Carcinogens; D009153:Mutagens; D020111:Chlorodiphenyl (54% Chlorine)