Rapid identification of biothreat and other clinically relevant bacterial species by use of universal PCR coupled with high-resolution melting analysis.
A rapid assay for eubacterial species identification is described using high-resolution melt analysis to characterize PCR products. Unique melt profiles generated from multiple hypervariable regions of the 16S rRNA gene for 100 clinically relevant bacterial pathogens, including category A and B biothreat agents and their surrogates, allowed highly specific species identification.
Yang
Samuel
S
Johns Hopkins University, Department of Emergency Medicine, Baltimore, MD 21209, USA. syang10@jhmi.edu
Ramachandran
Padmini
P
Rothman
Richard
R
Hsieh
Yu-Hsiang
YH
Hardick
Andrew
A
Won
Helen
H
Kecojevic
Aleksandar
A
Jackman
Joany
J
Gaydos
Charlotte
C
D001419:Bacteria / Q000302:isolation & purification*; D001424:Bacterial Infections / Q000175:diagnosis*; D023482:Bioterrorism; D004269:DNA, Bacterial / Q000235:genetics*; D004275:DNA, Ribosomal / Q000235:genetics; D004783:Environmental Microbiology; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D044366:Transition Temperature
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S