Up-regulation of ZO-1 expression and barrier function in cultured human corneal epithelial cells by substance P.
The effects of the sensory neurotransmitter substance P on the expression of tight junction proteins and on barrier function in human corneal epithelial cells were investigated. The expression of ZO-1, but not that of occludin or claudin-1, was increased by substance P in a concentration- and time-dependent manner. This effect was inhibited by the NK-1 receptor antagonist GR82334 and by KN62, an inhibitor of Ca(2+)- and calmodulin-dependent protein kinase II. Substance P also increased the transepithelial electrical resistance of a cell monolayer in a manner sensitive to GR82334. Substance P may therefore play a role in maintenance of tight junctions in the corneal epithelium.
Ko
Ji-Ae
JA
Department of Ophthalmology, Yamaguchi University Graduate School of Medicine, Ube City, Yamaguchi, Japan. jiae0831@yamaguchi-u.ac.jp
Yanai
Ryoji
R
Nishida
Teruo
T
D019307:1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine / Q000031:analogs & derivatives / Q000494:pharmacology; D001483:Base Sequence; D054732:Calcium-Calmodulin-Dependent Protein Kinase Type 2 / Q000037:antagonists & inhibitors; D002478:Cells, Cultured; D062445:Claudin-1; D017931:DNA Primers / Q000235:genetics; D017097:Electric Impedance; D019573:Epithelium, Corneal / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism*; D006801:Humans; D008565:Membrane Proteins / Q000235:genetics* / Q000378:metabolism*; D064729:Neurokinin-1 Receptor Antagonists; D062793:Occludin; D010750:Phosphoproteins / Q000235:genetics* / Q000378:metabolism*; D010803:Physalaemin / Q000031:analogs & derivatives / Q000494:pharmacology; D013373:Substance P / Q000378:metabolism / Q000494:pharmacology*; D019108:Tight Junctions / Q000187:drug effects / Q000378:metabolism; D015854:Up-Regulation / Q000187:drug effects; D062826:Zonula Occludens-1 Protein
C568108:CLDN1 protein, human; D062445:Claudin-1; D017931:DNA Primers; D008565:Membrane Proteins; D064729:Neurokinin-1 Receptor Antagonists; C568985:OCLN protein, human; D062793:Occludin; D010750:Phosphoproteins; C569042:TJP1 protein, human; D062826:Zonula Occludens-1 Protein; C079014:GR 82334; D010803:Physalaemin; D013373:Substance P; C063302:KN 62; D019307:1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine; D054732:Calcium-Calmodulin-Dependent Protein Kinase Type 2