Application of an immunoaffinity-based preconcentration method for mass spectrometric analysis of the O-chain polysaccharide of Aeromonas salmonicida from in vitro- and in vivo-grown cells.
In this study, application of magnetic beads (Dynabeads) coated with Aeromonas salmonicida lipopolysaccharide-specific polyclonal antisera to MS-based characterization of bacterial lipopolysaccharides has been evaluated. The results showed that the affinity-based preconcentration strategy resulted in at least a 100-fold increase in the detection of sensitivity, affording direct capillary electrophoresis (CE)-MS analysis of A. salmonicida lipopolysaccharide O-chain polysaccharide from in vitro-cultured cells. Subsequent CE-MS analysis of in vivo-grown cells of A. salmonicida confirmed significant changes in the structure of the lipopolysaccharide O-chain polysaccharide as a result of in vivo cultivation.
Wang
Zhan
Z
Institute for Biological Sciences, National Research Council of Canada, Ottawa, ON, Canada.
Liu
Xin
X
Garduño
Elizabeth
E
Garduño
Rafael A
RA
Li
Jianjun
J
Altman
Eleonora
E
D048409:Aeromonas salmonicida / Q000737:chemistry / Q000254:growth & development*; D000818:Animals; D003470:Culture Media; D019075:Electrophoresis, Capillary / Q000379:methods*; D005393:Fish Diseases / Q000382:microbiology; D018189:Immunomagnetic Separation / Q000379:methods*; D013058:Mass Spectrometry / Q000379:methods*; D019081:O Antigens / Q000032:analysis*; D011135:Polysaccharides, Bacterial / Q000737:chemistry; D012474:Salmon / Q000382:microbiology*; D012680:Sensitivity and Specificity
D003470:Culture Media; D019081:O Antigens; D011135:Polysaccharides, Bacterial