Antibacterial activity of methyl gallate isolated from Galla Rhois or carvacrol combined with nalidixic acid against nalidixic acid resistant bacteria.
Methyl gallate is a major component of Galla Rhois, as carvacrol is of oregano essential oils. Both have shown good antibacterial activity against intestinal bacteria. This study investigated the antibacterial activities of nalidixic acid in combination with methyl gallate and carvacrol against nalidixic acid resistant bacteria. The combined effect of nalidixic acid with methyl gallate and carvacrol was evaluated using the checkerboard method to obtain a fractional inhibitory concentration index. The results showed that the combinations of nalidixic acid + methyl gallate/carvacrol improved nalidixic acid resistant pathogenic bacteria inhibition with synergy or partial synergy activity. Thus, a strong bactericidal effect of the drug combinations was observed. In vitro data thus suggested that nalidixic acid combined with methyl gallate and carvacrol may be microbiologically beneficial, rather than antagonists.
Choi
Jang-Gi
JG
Department of Oriental Pharmacy, College of Pharmacy, Wonkwang University, Wonkwang Oriental Medicines Research Institute, Jeonbuk, Korea. jj0038@wku.ac.kr
Kang
Ok-Hwa
OH
Lee
Young-Seob
YS
Oh
You-Chang
YC
Chae
Hee-Sung
HS
Jang
Hye-Jin
HJ
Shin
Dong-Won
DW
Kwon
Dong-Yeul
DY
D000900:Anti-Bacterial Agents / Q000302:isolation & purification / Q000494:pharmacology; D001419:Bacteria / Q000187:drug effects*; D000081007:Cymenes; D024881:Drug Resistance, Bacterial / Q000187:drug effects*; D004359:Drug Therapy, Combination; D005707:Gallic Acid / Q000031:analogs & derivatives* / Q000302:isolation & purification / Q000494:pharmacology; D006801:Humans; D055670:Medicine, Korean Traditional; D008826:Microbial Sensitivity Tests; D039821:Monoterpenes / Q000494:pharmacology*; D009268:Nalidixic Acid / Q000494:pharmacology*; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology; D010946:Plants, Medicinal / Q000737:chemistry*
D000900:Anti-Bacterial Agents; D000081007:Cymenes; D039821:Monoterpenes; D010936:Plant Extracts; D009268:Nalidixic Acid; C052082:methyl gallate; D005707:Gallic Acid; C073316:carvacrol