title: Cytoprotective effects of lindenenyl acetate isolated from Lindera strychnifolia on mouse hippocampal HT22 cells.
abstract: Oxidative injury contributes to neuronal degeneration in many central nervous system (CNS) diseases, such as Parkinson's disease, Alzheimer's disease, epilepsy and ischemia. Inducible heme oxygenase (HO)-1 acts against oxidants that are thought to play a role in the pathogenesis of these diseases. Lindenenyl acetate, isolated by bioassay-guided fractionation of the MeOH extract of the roots of Lindera strychnifolia, showed potent neuroprotective effects on glutamate-induced neurotoxicity by inducing the expression of HO-1 and increasing the activity of HO in mouse hippocampal HT22 cells. Furthermore, lindenenyl acetate caused the nuclear accumulation of nuclear factor-E2-related factor 2 (Nrf2) and increased the promoter activity of antioxidant response elements (ARE) in mouse hippocampal HT22 cells. In addition, we found that treatment of the cells with extracellular signal-regulated kinase (ERK) inhibitor (U0126) reduced lindenenyl acetate-induced HO-1 expression. Lindenenyl acetate also increased ERK phosphorylation. These results suggest that lindenenyl acetate increases cellular resistance to glutamate-induced oxidative injury in mouse hippocampal HT22 cells, presumably through the ERK pathway-Nrf2/ARE-dependent HO-1 expression.
authors.affiliation: College of Pharmacy, Wonkwang University, Iksan 570-749, Republic of Korea.
authors.forename: Bin
authors.initials: B
authors.lastname: Li
authors.forename: Gil-Saeng
authors.initials: GS
authors.lastname: Jeong
authors.forename: Dae-Gill
authors.initials: DG
authors.lastname: Kang
authors.forename: Ho-Sub
authors.initials: HS
authors.lastname: Lee
authors.forename: Youn-Chul
authors.initials: YC
authors.lastname: Kim
mesh_terms: D000085:Acetates / Q000302:isolation & purification* / Q000494:pharmacology*; D021581:Active Transport, Cell Nucleus / Q000187:drug effects; D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D004789:Enzyme Activation / Q000187:drug effects; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism; D005452:Fluoresceins / Q000378:metabolism; D005453:Fluorescence; D005786:Gene Expression Regulation / Q000187:drug effects; D018698:Glutamic Acid / Q000633:toxicity; D051547:Heme Oxygenase-1 / Q000378:metabolism; D006624:Hippocampus / Q000166:cytology* / Q000187:drug effects* / Q000378:metabolism; D027443:Lindera / Q000737:chemistry*; D020935:MAP Kinase Signaling System / Q000187:drug effects; D051379:Mice; D051267:NF-E2-Related Factor 2 / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects; D018517:Plant Roots / Q000737:chemistry; D012717:Sesquiterpenes / Q000302:isolation & purification* / Q000494:pharmacology*
chemical_list: D000085:Acetates; D000975:Antioxidants; D005452:Fluoresceins; D051267:NF-E2-Related Factor 2; D012717:Sesquiterpenes; C543034:lindenenyl acetate; C029569:diacetyldichlorofluorescein; D018698:Glutamic Acid; D051547:Heme Oxygenase-1; D048049:Extracellular Signal-Regulated MAP Kinases