Construction and characterization of three lactate dehydrogenase-negative Enterococcus faecalis V583 mutants.
The roles of the two ldh genes of Enterococcus faecalis were studied using knockout mutants. Deletion of ldh-1 causes a metabolic shift from homolactic fermentation to ethanol, formate, and acetoin production, with a high level of formate production even under aerobic conditions. Ldh-2 plays only a minor role in lactate production.
Jönsson
Maria
M
Department of Chemistry, Biotechnology and Food Science, The Norwegian University of Life Sciences, P.O. Box 5003, N-1432 As, Norway. maria.jonsson@umb.no
Saleihan
Zhian
Z
Nes
Ingolf F
IF
Holo
Helge
H
D000093:Acetoin / Q000378:metabolism; D000332:Aerobiosis; D001426:Bacterial Proteins / Q000235:genetics*; D013293:Enterococcus faecalis / Q000201:enzymology* / Q000235:genetics*; D000431:Ethanol / Q000378:metabolism; D005561:Formates / Q000378:metabolism; D055786:Gene Knockout Techniques; D007770:L-Lactate Dehydrogenase / Q000172:deficiency*; D007773:Lactates / Q000378:metabolism; D053858:Metabolic Networks and Pathways; D008954:Models, Biological
D001426:Bacterial Proteins; D005561:Formates; D007773:Lactates; D000431:Ethanol; D000093:Acetoin; D007770:L-Lactate Dehydrogenase