title: Effective adsorption and separation of lysozyme with PAA-modified Fe3O4@silica core/shell microspheres.
abstract: Submicron-size Fe(3)O(4)@silica core/shell microspheres were prepared by sol-gel method. Through coating inert silica shell and adjusting the thickness, the dispersibility of magnetic microspheres in water was greatly improved. The resultant Fe(3)O(4)@silica microspheres with controllable saturation magnetization between 34.3 emu/g and 47.3 emu/g were superparamagnetic. All as-prepared magnetic composite microspheres possessed fleetly magnetic responsivity. The PAA-modified Fe(3)O(4)@silica microspheres were used for selective adsorption and separation of lysozyme, and the experimental results showed that the maximum binding capacity is about 127 mg/g. After surface modification of Fe(3)O(4)@silica with polyacrylic acid (PAA) flexible chains, the binding capacity of Fe(3)O(4)@silica-g-PAA microspheres for lysozyme were 22 times as much as that of pure Fe(3)O(4)@silica microspheres at the same pH condition.
authors.affiliation: Key Laboratory of Molecular Engineering of Polymers (Ministry of Education), Department of Macromolecular Science, Fudan University, Shanghai 200433, China.
authors.forename: Dandan
authors.initials: D
authors.lastname: Shao
authors.forename: Keke
authors.initials: K
authors.lastname: Xu
authors.forename: Xiaojie
authors.initials: X
authors.lastname: Song
authors.forename: Jianhua
authors.initials: J
authors.lastname: Hu
authors.forename: Wuli
authors.initials: W
authors.lastname: Yang
authors.forename: Changchun
authors.initials: C
authors.lastname: Wang
mesh_terms: D000180:Acrylic Resins / Q000737:chemistry*; D000327:Adsorption; D005290:Ferric Compounds / Q000737:chemistry*; D008280:Magnetics; D008722:Methods; D008863:Microspheres; D009113:Muramidase / Q000302:isolation & purification*; D010316:Particle Size; D044367:Phase Transition; D012822:Silicon Dioxide / Q000737:chemistry*
chemical_list: D000180:Acrylic Resins; D005290:Ferric Compounds; C000499:ferric oxide; C006903:carbopol 940; D012822:Silicon Dioxide; D009113:Muramidase