title: Effect of temperature on the development of Steinernema carpocapsae and Steinernema feltiae (Nematoda: Rhabditida) in liquid culture.
abstract: For commercial use of the entomopathogenic nematodes Steinernema carpocapsae and Steinernema feltiae in biological control of insect pests, they are produced in liquid culture on artificial media pre-incubated with their symbiotic bacteria Xenorhabdus nematophila and Xenorhabdus bovienii, respectively. After 1 day of the bacterial culture, nematode dauer juveniles (DJs) are inoculated, which recover development. The adult nematodes produce DJ offspring, which are harvested and can be sprayed. This study determined optimal temperatures to obtain high DJ progeny within a short process time. Temperatures assessed were 23 degrees C, 25 degrees C, 27 degrees C, and 29 degrees C for S. carpocapsae and 20 degrees C, 23 degrees C, 25 degrees C, and 27 degrees C for S. feltiae. The recovery of inoculated DJs was hardly affected and was reduced only in S. carpocapsae at 29 degrees C. The fecundity (eggs in uterus) in S. carpocapsae reached a maximum at 27 degrees C; whereas, maximum yields were recorded at 25 degrees C. For both Steinernema spp., highest DJ densities were obtained after 15 days incubation at 25 degrees C. Optimal culture temperature for both nematode species is 25 degrees C. S. carpocapsae was more sensible to suboptimal temperature than S. feltiae. Results on total DJ density and DJ proportion of the total nematode population were more variable at non-optimal temperature condition for S. carpocapsae than for S. feltiae. Suboptimal culture temperature also reduced DJ infectivity.
authors.affiliation: Institute for Phytopathology, Department for Biotechnology and Biological Control, Christian-Albrechts-University Kiel, Germany.
authors.forename: A
authors.initials: A
authors.lastname: Hirao
authors.forename: R-U
authors.initials: RU
authors.lastname: Ehlers
mesh_terms: D000818:Animals; D003470:Culture Media; D005260:Female; D005298:Fertility; D008297:Male; D010572:Pest Control, Biological / Q000191:economics / Q000379:methods; D017168:Rhabditida / Q000254:growth & development* / Q000382:microbiology; D013559:Symbiosis; D013696:Temperature; D020593:Xenorhabdus / Q000254:growth & development
chemical_list: D003470:Culture Media