Liquid chromatography/tandem mass spectrometry for the determination of changrolin in rat plasma: application to a bioavailability study.
A sensitive and specific LC-CMS/MS method was developed for the quantification of changrolin, an anti-arrhythmic drug, in rat plasma using tiapride as internal standard. Liquid-liquid extraction was employed for sample preparation and analyzed using a multiple reaction monitoring mode with electrospray positive ionization source. The calibration curve for changrolin was linear over the range 5-1600 ng/mL with coefficients of correlation >0.99. The intra- and inter-batch precision was less than 8.6%, and accuracy ranged from 94.6% to 104.5%. This validated LC-MS/MS method was successfully applied to a bioavailability study of oral and intravenous administration of changrolin with 20mg/kg dosage in SD rats.
Yang
Ding
D
State Key Laboratory of Drug Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China.
Yu
Chen
C
Li
Shuijun
S
Liu
Gangyi
G
Lu
Youli
Y
Wang
Yiping
Y
D000818:Animals; D000889:Anti-Arrhythmia Agents / Q000097:blood* / Q000493:pharmacokinetics; D001682:Biological Availability; D002138:Calibration; D002853:Chromatography, Liquid / Q000379:methods*; D011799:Quinazolines / Q000097:blood* / Q000493:pharmacokinetics; D051381:Rats; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D053719:Tandem Mass Spectrometry / Q000379:methods*
D000889:Anti-Arrhythmia Agents; D011799:Quinazolines; C022128:changrolin