[Observation on the ultrastructure of Taenia solium oncosphere].
OBJECTIVE
To observe the ultrastructure of Taenia solium oncospheres.


METHODS
Patients infected with Taenia solium were de-wormed by decoction arecae and pumpkin seeds to get the mature proglottids and collect eggs. The eggs were treated with sodium hypochlorite to break the eggshells. Oncospheres were collected in Percoll (isoosmotic solution), and activated with artificial intestinal juice. The specimens were prepared with hot agar centrifugation for ultra-thin sections and observed with transmission electron microscopy (TEM) .


RESULTS
T. solium oncosphere was in oval shape with a size of (14-17) microm x (10-13) microm. There were some irregular ecphymata or plicae on its surface. The hooks were composed of outer pellet layer, the middle fibrous layer and the central core marrow. Myoblasts, hook-forming cells and cerebral cortex cells were observed in the mature oncospheres.


CONCLUSIONS
The ultrastructure of Taenia solium oncosphere is similar to that of Hymenolepis diminuta, with difference in hooks. There are binucleate cells which play a role in forming epithelium in the development of oncospheres.
Sun
Xiao-lin
XL
College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, China.
Cai
Xue-peng
XP
Jing
Zhi-zhong
ZZ
Mi
Xiao-yun
XY
Wang
Pei-ya
PY
Chen
Huai-tao
HT
D000818:Animals; D006801:Humans; D007814:Larva / Q000648:ultrastructure; D041201:Taenia solium / Q000196:embryology* / Q000648:ultrastructure*