title: [Identification of Wnt/beta-catenin signal pathway in human prostate cancer cell lines and its significance].
abstract: OBJECTIVE
  To determine the Wnt/beta-catenin signal pathway in different human prostate cancer cell lines and explore its role in epithelial-mesenchymal transition (EMT) in human prostate cancer.
  
  
  METHODS
  We detected the expressions of beta-catenin, t-GSK3beta and p-GSK3beta in several prostate cancer cell lines (LNCaP, C4, C4-2, C4-2B, IF11, IA8, PC-3 and DU145) with different characteristics of epithelial-mesenchymal transition (EMT) by Western blotting.
  
  
  RESULTS
  There were remarkable differences in the expressions of beta-catenin and p-GSK3beta among the cell lines, with beta-catenin and p-GSK3beta highly expressed in LNCaP, C4, C4-2, C4-2B, IF11 and IA8, lowly expressed in PC-3 and DU145, but no difference was observed in the expressions of t-GSK3beta in all the cell lines.
  
  
  CONCLUSIONS
  There are differences in the state of the Wnt/beta-catenin signal pathway among the cell lines with different characters of EMT.
authors.affiliation: Department of Urology, Beijing Anzhen Hospital Affiliated to Capital Medical University, Beijing 100029, China. yongguangjiang@yahoo.com.cn
authors.forename: Yong-Guang
authors.initials: YG
authors.lastname: Jiang
authors.forename: Jia-Hui
authors.initials: JH
authors.lastname: Zhao
authors.forename: Yong
authors.initials: Y
authors.lastname: Luo
authors.forename: Da-Lin
authors.initials: DL
authors.lastname: He
authors.forename: Xin-Yang
authors.initials: XY
authors.lastname: Wang
authors.forename: Xin-Hao
authors.initials: XH
authors.lastname: Cui
mesh_terms: D045744:Cell Line, Tumor; D006801:Humans; D008297:Male; D011471:Prostatic Neoplasms / Q000378:metabolism*; D015398:Signal Transduction; D051153:Wnt Proteins / Q000378:metabolism*; D051176:beta Catenin / Q000378:metabolism*
chemical_list: D051153:Wnt Proteins; D051176:beta Catenin