[Alpha-glucosidase inhibitors from Luculia pinciana].
OBJECTIVE
To search alpha-glucosidase inhibitors from Luculia pinciana.


METHODS
The alpha-glucosidase inhibitor was isolated by the column chromatographic techniques and the bioassay-guided method in vitro. A combination of MS and NMR spectroscopy was used to identify the chemical structures. The inhibitory kinetic of the isolations was also investigated.


RESULTS
The ethyl acetate extract showed strongest inhibitory activity, and five active compounds were isolated and identified as scopletin (1), 5-methoxy-8-hydroxycoumarin (2), lalpha,3beta,24-trihydroxyursolic acid (3), ursolic acid (4) and oleanolic acid (5). The IC50 values of all compounds were lower than that of acarbose. Four of them shown noncompetitive type manner on alpha-glucosidase inhibition except that compound 3 is competitive type manner.


CONCLUSIONS
Compounds 1-4 as the inhibitors of alpha-glucosidase were reported for the first time.
Kang
Wenyi
W
Institute of Natural Products, Henan University, Kaifeng 475004, China. kangweny@hotmail.com
Zhang
Li
L
Song
Yanli
Y
D020909:Acarbose / Q000494:pharmacology; D003374:Coumarins / Q000494:pharmacology; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D065089:Glycoside Hydrolase Inhibitors; D020128:Inhibitory Concentration 50; D009828:Oleanolic Acid / Q000302:isolation & purification / Q000494:pharmacology*; D010936:Plant Extracts / Q000494:pharmacology; D019666:Rubiaceae / Q000737:chemistry*; D014315:Triterpenes / Q000302:isolation & purification / Q000494:pharmacology*; D000520:alpha-Glucosidases / Q000378:metabolism; D000097245:Ursolic Acid
D020909:Acarbose; D003374:Coumarins; D004791:Enzyme Inhibitors; D065089:Glycoside Hydrolase Inhibitors; D009828:Oleanolic Acid; D010936:Plant Extracts; D014315:Triterpenes; D000520:alpha-Glucosidases; D000097245:Ursolic Acid; C049536:8-hydroxycoumarin