title: Phosphorylation status of nuclear ribosomal protein S3 is reciprocally regulated by protein kinase C{delta} and protein phosphatase 2A.
abstract: It has been shown previously that ribosomal protein S3 (rpS3) has an endonuclease activity, which is increased by protein kinase Cdelta (PKCdelta)-dependent phosphorylation. However, the reciprocal mechanism for rpS3 dephosphorylation is not known. In this study, we examined phosphatases involved in rpS3 dephosphorylation, and we determined that rpS3 is specifically dephosphorylated by protein phosphatase 2A (PP2A). By immunoprecipitation assay, rpS3 only interacted with PP2Ac but not with protein phosphatase 1. The interaction between rpS3 and PP2Ac occurred only in the nuclear fraction. Moreover, the PP2Ac association with rpS3 was identified in cells transfected with wild-type rpS3 but not with mutant rpS3 lacking PKCdelta phosphorylation sites. PP2A inhibition using okadaic acid induced rpS3 phosphorylation. The level of phosphorylated rpS3 in cells was decreased by the overexpression of PP2Ac and was increased by the down-regulation of PP2Ac. Taken together, these results suggest that oxidative stress regulates the phosphorylation status of nonribosomal rpS3 by both activating PKCdelta and blocking the PP2A interaction with rpS3.
authors.affiliation: Laboratory of Biochemistry, School of Life Sciences and Biotechnology, and BioInstitute, Korea University, Seoul 136-701, Republic of Korea.
authors.forename: Tae-Sung
authors.initials: TS
authors.lastname: Kim
authors.forename: Hag Dong
authors.initials: HD
authors.lastname: Kim
authors.forename: Hyun-Seock
authors.initials: HS
authors.lastname: Shin
authors.forename: Joon
authors.initials: J
authors.lastname: Kim
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D002467:Cell Nucleus / Q000378:metabolism*; D015971:Gene Expression Regulation, Enzymologic; D006801:Humans; D047468:Immunoprecipitation; D051379:Mice; D009154:Mutation; D041681:NIH 3T3 Cells; D018384:Oxidative Stress; D010766:Phosphorylation; D051745:Protein Kinase C-delta / Q000378:metabolism*; D054648:Protein Phosphatase 2 / Q000378:metabolism*; D017434:Protein Structure, Tertiary; D012269:Ribosomal Proteins / Q000378:metabolism*
chemical_list: D012269:Ribosomal Proteins; C044152:ribosomal protein S3; D051745:Protein Kinase C-delta; D054648:Protein Phosphatase 2