title: Modulatory role of Emblica officinalis against alcohol induced biochemical and biophysical changes in rat erythrocyte membranes.
abstract: This study investigated the protective effect of Emblica officinalis against alcohol-induced biochemical and biophysical changes in rat erythrocyte membranes. Thirty-two male rats were divided into four groups (n=8 in each group): control (C), alcohol (A), alcohol plus Emblica fruit extract (A+EFE) and Emblica fruit extract (EFE) alone. Administration of twenty percent alcohol (5 g/kg body weight) to rats significantly increased cholesterol/phospholipid (C/P) ratio, lipid peroxidation and the activities of Na(+)/K(+) and Mg(2+) ATPases in erythrocyte membranes as well as augmented nitric oxide (NO) levels. However, membrane fluidity studies using the fluorescent probe DPH (1,6 diphenyl 1,3 hexatriene) reveals that alcohol administration significantly (p<0.05) increased membrane anisotropic values and altered membrane individual phospholipid content. Administration of EFE (250 mg/kg body weight) to alcoholic rats resulted in significant (p<0.05) reduction of NO levels, erythrocyte membrane lipid peroxidation, C/P ratio, activities of Na(+)/K(+) and Mg(2+) ATPases and fluorescent anisotropic values. Further, EFE administration to alcoholic rats beneficially modulated membrane properties as evidenced from the contents of total phospholipids as well individual phospholipid classes. The tannoid principles present in Emblica offers protection against alcohol induced adverse effects in rats.
authors.affiliation: Department of Biochemistry, Sri Krishnadevaraya University, Anantapur 515 003, India.
authors.forename: Vaddi Damodara
authors.initials: VD
authors.lastname: Reddy
authors.forename: Pannuru
authors.initials: P
authors.lastname: Padmavathi
authors.forename: Maturu
authors.initials: M
authors.lastname: Paramahamsa
authors.forename: Nallanchakravarthula
authors.initials: N
authors.lastname: Varadacharyulu
mesh_terms: D000818:Animals; D016880:Anisotropy; D002492:Central Nervous System Depressants / Q000037:antagonists & inhibitors* / Q000633:toxicity*; D002784:Cholesterol / Q000097:blood; D004161:Diphenylhexatriene / Q000737:chemistry; D004910:Erythrocyte Membrane / Q000737:chemistry / Q000187:drug effects* / Q000502:physiology; D000431:Ethanol / Q000037:antagonists & inhibitors* / Q000633:toxicity*; D019665:Euphorbiaceae / Q000737:chemistry*; D005456:Fluorescent Dyes; D008297:Male; D008560:Membrane Fluidity / Q000187:drug effects; D008563:Membrane Lipids / Q000737:chemistry; D009566:Nitrates / Q000097:blood; D009569:Nitric Oxide / Q000097:blood; D009573:Nitrites / Q000097:blood; D010743:Phospholipids / Q000097:blood; D051381:Rats; D017208:Rats, Wistar; D013050:Spectrometry, Fluorescence
chemical_list: D002492:Central Nervous System Depressants; D005456:Fluorescent Dyes; D008563:Membrane Lipids; D009566:Nitrates; D009573:Nitrites; D010743:Phospholipids; D004161:Diphenylhexatriene; D009569:Nitric Oxide; D000431:Ethanol; D002784:Cholesterol