Advanced glycation end product accumulation in rho(0) cells without a functional respiratory chain.
Advanced glycation end products (AGEs) accumulate during ageing with reactive oxygen species from the mitochondrial respiratory chain discussed as a driving force. To determine the role of mitochondrial activity for AGE formation, a rho(0) derivative of the 143B.TK(-) osteosarcoma cell line lacking the respiratory chain, was analysed. These cells exhibit decreased superoxide formation but unchanged mitochondrial SOD expression as well as unchanged antioxidative free sulfhydryl (SH) levels. Whereas total protein content shows no differences in AGE levels, cell fractionation and Western blotting demonstrates some changes in the AGE pattern. Thus, the absence of functional respiration has only a negligible impact on AGE accumulation.
Nass
Norbert
N
Department of Cardiovascular Surgery, Martin Luther University Halle-Wittenberg, Halle/Saale, Germany. norbert.nass@medizin.uni-halle.de
Kukat
Alexandra
A
Seibel
Peter
P
Brömme
Hans-Jürgen
HJ
Schinzel
Reinhard
R
Silber
Rolf-Edgar
RE
Simm
Andreas
A
D015153:Blotting, Western; D045744:Cell Line, Tumor; D004579:Electron Transport / Q000502:physiology*; D017127:Glycation End Products, Advanced / Q000378:metabolism*; D006801:Humans; D008928:Mitochondria / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D013438:Sulfhydryl Compounds / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism
D017127:Glycation End Products, Advanced; D017382:Reactive Oxygen Species; D013438:Sulfhydryl Compounds; D013482:Superoxide Dismutase