Imcroporin, a new cationic antimicrobial peptide from the venom of the scorpion Isometrus maculates.
The pace of resistance against antibiotics almost exceeds that of the development of new drugs. As many bacteria have become resistant to conventional antibiotics, new drugs or drug resources are badly needed to combat antibiotic-resistant pathogens, like methicillin-resistant Staphylococcus aureus (MRSA). Antimicrobial peptides, rich sources existing in nature, are able to effectively kill multidrug-resistant pathogens. Here, imcroporin, a new antimicrobial peptide, was screened and isolated from the cDNA library of the venomous gland of Isometrus maculates. The MIC of imcroporin against MRSA was 50 microg/ml, 8-fold lower than that of cefotaxime and 40-fold lower than that of penicillin. Imcroporin killed bacteria rapidly in vitro, inhibited bacterial growth, and cured infected mice. These results revealed that imcroporin could be considered a potential anti-infective drug or lead compound, especially for treating antibiotic-resistant pathogens.
Zhao
Zhenhuan
Z
State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Hubei, 430072, China.
Ma
Yibao
Y
Dai
Chao
C
Zhao
Ruiming
R
Li
SongRyong
S
Wu
Yingliang
Y
Cao
Zhijian
Z
Li
Wenxin
W
D000595:Amino Acid Sequence; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology / Q000627:therapeutic use; D023181:Antimicrobial Cationic Peptides / Q000378:metabolism / Q000494:pharmacology* / Q000627:therapeutic use*; D002439:Cefotaxime / Q000494:pharmacology / Q000627:therapeutic use; D005260:Female; D006461:Hemolysis / Q000187:drug effects; D006801:Humans; D008297:Male; D055624:Methicillin-Resistant Staphylococcus aureus / Q000187:drug effects; D051379:Mice; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D010406:Penicillins / Q000494:pharmacology / Q000627:therapeutic use; D012604:Scorpion Venoms / Q000378:metabolism / Q000494:pharmacology* / Q000627:therapeutic use*; D012605:Scorpions / Q000737:chemistry / Q000378:metabolism*; D017386:Sequence Homology, Amino Acid; D013203:Staphylococcal Infections / Q000188:drug therapy; D014640:Vancomycin / Q000494:pharmacology / Q000627:therapeutic use
D000900:Anti-Bacterial Agents; D023181:Antimicrobial Cationic Peptides; D010406:Penicillins; D012604:Scorpion Venoms; D014640:Vancomycin; D002439:Cefotaxime