(1)H NMR based metabonomics of human amniotic fluid for the metabolic characterization of fetus malformations.
An NMR-metabonomic study of malformed fetuses was carried out through human amniotic fluid (HAF) analysis. Over 70 compounds were detected in control HAF by NMR. Possible confounding variables (fetus gender and gestational and maternal ages) were shown not to induce detectable compositional trends in the control group considered. Malformed fetuses showed variations in glucose, some amino acids and organic acids and proteins. In tandem with enzymatic assays, these NMR results suggest that changes in gycolysis and gluconeogenesis as well as kidney underdevelopment occur in the malformed fetuses studied here.
Graça
Gonçalo
G
CICECO, Department of Chemistry, University of Aveiro, Portugal.
Duarte
Iola F
IF
Barros
António S
AS
Goodfellow
Brian J
BJ
Diaz
Sílvia
S
Carreira
Isabel M
IM
Couceiro
Ana Bela
AB
Galhano
Eulália
E
Gil
Ana M
AM
D000293:Adolescent; D000328:Adult; D000596:Amino Acids / Q000378:metabolism; D000641:Ammonia / Q000378:metabolism; D000653:Amniotic Fluid / Q000378:metabolism*; D005260:Female; D005333:Fetus / Q000002:abnormalities* / Q000378:metabolism; D005947:Glucose / Q000378:metabolism; D006801:Humans; D055442:Metabolome; D055432:Metabolomics / Q000379:methods*; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods*; D011247:Pregnancy; D025341:Principal Component Analysis
D000596:Amino Acids; D000641:Ammonia; D005947:Glucose