Gene expression profiles of the one-carbon metabolism pathway.
One-carbon metabolism plays a critical role in both DNA methylation and DNA synthesis. Accumulating evidence has shown that interruptions of this pathway are associated with many disease outcomes including cardiovascular diseases and cancers. Mechanistic studies have been performed on genetic polymorphisms involved in one-carbon metabolism. However, expression profiles of these inter-related genes are not well-known. In this study, we examined the gene expression profiles of 11 one-carbon metabolizing genes by quantifying the mRNA level of the lymphocyte among 54 healthy individuals and explored the correlations of these genes. We found these genes were expressed in lymphocytes at moderate levels and showed significant inter-person variations. We also applied principle component analysis to explore potential patterns of expression. The components identified by the program agreed with existing knowledge about one-carbon metabolism. This study helps us better understand the biological functions of one-carbon metabolism.
Lee
Yin Leng
YL
Department of Community and Preventive Medicine, Mount Sinai School of Medicine, New York 10029, USA.
Xu
Xinran
X
Wallenstein
Sylvan
S
Chen
Jia
J
D002478:Cells, Cultured; D005260:Female; D018628:Gene Dosage; D020869:Gene Expression Profiling; D014644:Genetic Variation; D006801:Humans; D008214:Lymphocytes / Q000201:enzymology*; D008297:Male; D053858:Metabolic Networks and Pathways; D019875:One-Carbon Group Transferases / Q000235:genetics* / Q000378:metabolism*; D011110:Polymorphism, Genetic
D019875:One-Carbon Group Transferases