Electrostatically mediated liposome fusion and lipid exchange with a nanoparticle-supported bilayer for control of surface charge, drug containment, and delivery.
The loading and containment of cargo within nanoparticles and their efficient delivery to cells represent a primary challenge in nanomedicine. We report lipid exchange between free and mesoporous silica nanoparticle-supported lipid bilayers as an effective means of containing cargo, controlling charge, and directing delivery to mammalian cells. The delivery of a membrane-impermeable dye (calcein) and a chemotherapeutic drug (doxorubicin) are demonstrated. Exchanged lipid bilayers minimized premature drug release, and an overall positive charge on the supported lipid bilayer effected enhanced delivery.
Liu
Juewen
J
Center for Micro-Engineered Materials, University of New Mexico, Albuquerque, New Mexico 87106, USA.
Jiang
Xingmao
X
Ashley
Carlee
C
Brinker
C Jeffrey
CJ
D000818:Animals; D016466:CHO Cells; D006224:Cricetinae; D003412:Cricetulus; D004317:Doxorubicin / Q000008:administration & dosage / Q000737:chemistry; D016503:Drug Delivery Systems / Q000379:methods*; D005229:Fatty Acids, Monounsaturated / Q000737:chemistry; D005452:Fluoresceins / Q000008:administration & dosage / Q000737:chemistry / Q000493:pharmacokinetics; D008051:Lipid Bilayers / Q000737:chemistry*; D008081:Liposomes / Q000008:administration & dosage / Q000737:chemistry*; D053758:Nanoparticles / Q000008:administration & dosage / Q000737:chemistry*; D000644:Quaternary Ammonium Compounds / Q000737:chemistry; D012822:Silicon Dioxide / Q000737:chemistry; D013050:Spectrometry, Fluorescence; D055672:Static Electricity; D013499:Surface Properties
D005229:Fatty Acids, Monounsaturated; D005452:Fluoresceins; D008051:Lipid Bilayers; D008081:Liposomes; D000644:Quaternary Ammonium Compounds; D012822:Silicon Dioxide; D004317:Doxorubicin; C070046:1,2-dioleoyloxy-3-(trimethylammonium)propane; C007740:fluorexon