A quantitative method for analyzing establishing-efficiency of persistent viral infection.
A quantitative method for analyzing establishing-efficiency of persistent infection was devised. The efficiency of hPIV2 CA and SV5 T1 strains was found to be high, that is, 0.1 approximately 0.3 (an efficiency of 1.0 indicates that 100% of the virus-infected cells became persistently infected). The efficiency of the SV5 WR strain was also high, approximately 0.1, though the virus had no ability to immediately establish a steady state of persistent infection in whole cell-culture systems. At about 0.0007, the efficiency of SV41 was almost the same as that of the hPIV2 Toshiba strain. The establishing efficiencies of various rSeV were further analyzed in detail. The efficiencies of the rSeV(PA), rSeV(Ppi) and rSeV(HNpi) were below the limit of detection, while that of rSeV(Lpi) was nearly 1. Although the efficiency was around 0.001, the rSeV(Mpi) and the rSeV(Fpi) were unexpectedly found to be capable of forming persistently-infected cells, indicating that both the Fpi and Mpi proteins contribute to the establishing efficiency of persistent infection of SeVpi.
Ito
Morihiro
M
Department of Biomedical Sciences, College of Life and Health Sciences, Chubu University, Kasugai-City, Aichi-Prefecture 487-8501, Japan. m-ito@isc.chubu.ac.jp
Yamakawa
Izumi
I
Nishio
Machiko
M
Tsurudome
Masato
M
Kawano
Mitsuo
M
Komada
Hiroshi
H
Ito
Yasuhiko
Y
D000818:Animals; D002642:Chick Embryo; D002522:Chlorocebus aethiops; D006168:Guinea Pigs; D006367:HeLa Cells; D006801:Humans; D018184:Paramyxoviridae Infections / Q000821:virology*; D018098:Paramyxovirinae / Q000502:physiology*; D014709:Vero Cells; D014764:Viral Proteins / Q000032:analysis; D014776:Virus Cultivation / Q000379:methods*
D014764:Viral Proteins