Distribution of macrophages and T cells in syngrafts and allografts after experimental rat lung transplantation.
Macrophages and T cells have a pivotal role in orchestrating the acute lung allograft rejection response. We investigated the spatial and temporal distribution of these immune cells and the synthesis patterns of the T(h)1- and T(h)2-cytokine IL-12 and IL-10 during the early course after transplantation (Tx). Orthotopic single-lung Tx was performed in Lewis to Lewis (syngrafts) and Brown Norway/Lewis F(1) hybrid to Lewis (allografts). Naïve lungs, syngrafts after 5 days and allografts after 3 and 5 days were analyzed for CD68+, CD163+ and CD3+ cells by immunohistochemistry and IL-12 and IL-10 were detected by immunofluorescence. CD68+ macrophages increased in number after allogeneic Tx compared to syngeneic Tx on day 5 (P<.001), CD163+ macrophages sequestrated early around veins (day 3) compared to the accumulation around arteries and bronchioles (P<.001) while CD3+ T cells were scarce. There was a predominance of IL-12 over IL-10 on day 5 after allogeneic Tx (P<.001). CD68+ macrophages were the most abundant cells during acute pulmonary rejection and CD163+ macrophages showed a characteristic distribution pattern over time around vessels and bronchioles. The up-regulation of IL-12 reflects an early response after allo-antigen exposure, indicating a strong impact of the initiation of the T(h)1 pathway at an early phase during acute lung rejection.
Jungraithmayr
Wolfgang
W
Division of Thoracic Surgery, University Hospital Zurich, Raemistr. 100, CH-8091 Zurich, Switzerland. wolfgang.jungraithmayr@usz.ch
Inci
Ilhan
I
Bain
Martha
M
Hillinger
Sven
S
Korom
Stephan
S
Weder
Walter
W
D000818:Animals; D005455:Fluorescent Antibody Technique; D006084:Graft Rejection / Q000276:immunology; D007150:Immunohistochemistry; D016753:Interleukin-10 / Q000096:biosynthesis / Q000276:immunology; D018664:Interleukin-12 / Q000096:biosynthesis / Q000276:immunology; D016040:Lung Transplantation / Q000276:immunology*; D008264:Macrophages / Q000276:immunology*; D008297:Male; D051381:Rats; D011914:Rats, Inbred BN; D011916:Rats, Inbred F344; D016176:T-Lymphocyte Subsets / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology*; D014184:Transplantation, Homologous
D016753:Interleukin-10; D018664:Interleukin-12