Ex vivo diagnosis of lung cancer using a Raman miniprobe.
Lung cancer is the most common cause of cancer death. The conventional method of confirming the diagnosis is bronchoscopy, inspecting the airways of the patient with a fiber optic endoscope. A number of studies have shown that Raman spectroscopy can diagnose lung cancer in vitro. In this study, Raman spectra were obtained from ex vivo normal and malignant lung tissue using a minifiber optic Raman probe suitable for insertion into the working channel of a bronchoscope. Shifted subtracted Raman spectroscopy was used to reduce the fluorescence from the lung tissue. Using principal component analysis with a leave-one-out analysis, the tissues were classified accurately. This novel technique has the potential to obtain Raman spectra from tumors from patients with lung cancer in vivo.
Magee
Nicholas D
ND
Respiratory Medicine Research Group, Centre for Infection and Immunity, Microbiology Building, Queens University Belfast, Grosvenor Road, Belfast, BT12 6BN, United Kingdom. n.d.magee@qub.ac.uk
Villaumie
Julien S
JS
Marple
Eric T
ET
Ennis
Madeleine
M
Elborn
J Stuart
JS
McGarvey
John J
JJ
D006801:Humans; D008175:Lung Neoplasms / Q000175:diagnosis*; D013059:Spectrum Analysis, Raman / Q000295:instrumentation*