[Phylogenetic diversity of cultivable bacteria associated with a sea anemone from coast of the Naozhou island in Zhanjiang, China].
OBJECTIVE
To understand the diversity of cultivable bacteria isolated from a sea anemone collected from the coastal water of the Naozbou island in the Leizhou Bay on South China Sea.


METHODS
Bacteria were isolated from a sea anemone by using conventional culture-dependent method and investigated by using phylogenetic analysis based on 165 rRNA gene sequence comparisons.


RESULTS
We isolated 126 bacteria strains from the sample on marine agar 2216 (Difco), International Streotomyces Project medium 2 agar, nutrient agar, sea water agar and humic acid agar supplemented with 0-2 mol/L NaCl. Based on partial morphological, physiological and biochemical characteristics, we selected 42 strains for molecular systematic study based on 16S rRNA gene sequences. Our results showed that 42 isolates were members of eighteen genera (Alteromonas, Bacillus, Brachybacterium, Brevibacterium, Halobacillus, Halomonas, Nocardiopsis, Oceanobacillus, Piscibacillus, Planococcus, Pontibacillus, Pseudoalteromonas, Pseudonocardia, Salinicoccus, Salinivibrio, Staphylococcus, Vibrio, Virgibacillus) of eleven families (Alteromonadaceae, Bacillaceae, Brevibacteriaceae, Dermabacteraceae, Halomonadaceae, Planococcaceae, Pseudoalteromonadaceae, Pseudonocardiaceae, Nocardiopsaceae, Staphylococcaceae, Vibrionaceae) in three major phylogenetic groups (Actinobacteria, Firmicutes, Gamma-Proteobacteria). The most abundant and diverse isolates were within the phylum Firmicutes (40.5%) and the subphylum Gamma-Proteobacteria (33.3%). The phylogenetic distance matrix results suggested that, out of 42 isolates, 37 were different strains of 19 known species,and that at least 6 strains represented 6 new species within 6 characterized genera.


CONCLUSIONS
The results presented above showed that there were abundant species diversity and phylogenetic diversity of bacteria isolated from the sea anemone.
Xiao
Huaidong
H
Key Laboratory of Plant Resources Conservation and Utilization, Hunan Province, College of Biological Resource and Environment Science, Jishou University, Jishou 416000, China. xiaohuaidong@yahoo.com.cn
Chen
Yiguang
Y
Liu
Zhuxiang
Z
Huang
Ke
K
Li
Wenjun
W
Cui
Xiaolong
X
Zhang
Li
L
Yi
Langbo
L
D000818:Animals; D001419:Bacteria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D044822:Biodiversity; D002681:China; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012615:Sea Anemones / Q000382:microbiology*; D012623:Seawater / Q000382:microbiology*
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S