title: Assessment and use of two silicon carbide multi-well plates for library synthesis and proteolytic digests using microwave heating.
abstract: The use of two silicon carbide plates is reported for the preparation of three libraries of organic molecules using microwave heating. In addition, a preliminary study has been carried out, showing that one of the plates can also be used in a proteomics setting. Both the 24-position and 48-position plates heated evenly when irradiated with microwave energy. The 48-position plate was used to prepare a library of N-aryl functionalized beta-amino esters via an aza-Michael reaction between anilines and Michael acceptors. The 24-position plate was used to prepare a library of biaryls via a Suzuki coupling methodology and a library of 1,4-dihydropyridines via a Hantzsch synthesis. The 48-position plate was also used to perform the proteolytic digestion of insulin chain B by trypsin.
authors.affiliation: Department of Chemistry, University of Connecticut, 55 North Eagleville Rd, Storrs, CT 06269-3060, USA.
authors.forename: Lauren M
authors.initials: LM
authors.lastname: Stencel
authors.forename: Chad M
authors.initials: CM
authors.lastname: Kormos
authors.forename: Keri B
authors.initials: KB
authors.lastname: Avery
authors.forename: Nicholas E
authors.initials: NE
authors.lastname: Leadbeater
mesh_terms: D000588:Amines / Q000138:chemical synthesis / Q000737:chemistry; D000818:Animals; D017554:Carbon Compounds, Inorganic / Q000737:chemistry*; D002417:Cattle; D020650:Combinatorial Chemistry Techniques / Q000295:instrumentation*; D004095:Dihydropyridines / Q000138:chemical synthesis / Q000737:chemistry; D004867:Equipment Design; D004952:Esters / Q000138:chemical synthesis / Q000737:chemistry; D006841:Hydrocarbons, Aromatic / Q000138:chemical synthesis / Q000737:chemistry; D007328:Insulin / Q000378:metabolism; D008872:Microwaves; D040901:Proteomics / Q000295:instrumentation; D017655:Silicon Compounds / Q000737:chemistry*; D054852:Small Molecule Libraries / Q000138:chemical synthesis* / Q000737:chemistry; D013696:Temperature; D014357:Trypsin / Q000378:metabolism
chemical_list: D000588:Amines; D017554:Carbon Compounds, Inorganic; D004095:Dihydropyridines; D004952:Esters; D006841:Hydrocarbons, Aromatic; D007328:Insulin; D017655:Silicon Compounds; D054852:Small Molecule Libraries; C038806:1,4-dihydropyridine; D014357:Trypsin; C022088:silicon carbide