title: Expression of nucleus-encoded genes for chloroplast proteins in the flagellate Euglena gracilis.
abstract: Reverse transcription PCRs (RT-PCRs), real-time RT-PCRs and microarrays containing 50-mer oligonucleotides representing nucleus-encoded genes for chloroplast proteins from Euglena gracilis were used to compare light- and dark-grown wild-type mRNA levels to those of light- and dark-grown E. gracilis stable white mutant strains W(gm)ZOflL, W₃BUL and W₁₀BSmL. The analyses revealed no light-dependent regulation of mRNA levels. Moreover, the mRNA levels of most genes were unchanged in all white mutants in comparison with wild-type. These results suggest that mRNA levels of nucleus-encoded genes for chloroplast proteins in E. gracilis do not depend on either light or plastid function.
authors.affiliation: Institute of Cell Biology, Faculty of Natural Sciences, Comenius University, 84215 Bratislava, Slovakia.
authors.forename: Matej
authors.initials: M
authors.lastname: Vesteg
authors.forename: Rostislav
authors.initials: R
authors.lastname: Vacula
authors.forename: Suzanne
authors.initials: S
authors.lastname: Burey
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Löffelhardt
authors.forename: Hana
authors.initials: H
authors.lastname: Drahovská
authors.forename: William
authors.initials: W
authors.lastname: Martin
authors.forename: Juraj
authors.initials: J
authors.lastname: Krajcovic
mesh_terms: D001483:Base Sequence; D002467:Cell Nucleus / Q000235:genetics*; D002736:Chloroplasts / Q000737:chemistry / Q000378:metabolism*; D018742:DNA, Chloroplast / Q000235:genetics; D005056:Euglena gracilis / Q000166:cytology* / Q000235:genetics* / Q000378:metabolism; D008027:Light; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D018747:RNA, Chloroplast / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis
chemical_list: D018742:DNA, Chloroplast; D010940:Plant Proteins; D018747:RNA, Chloroplast; D012333:RNA, Messenger