title: Assessment of gene-nutrient interactions on inflammatory status of the elderly with the use of a zinc diet score--ZINCAGE study.
abstract: Although zinc plays an important role in health status of the elderly, their dietary habits in relation to zinc intake are not well documented. The main objective of the current study was the assessment of dietary zinc intake in European old populations and the investigation of its impact on plasma zinc and inflammatory cytokines concentrations, in relation to genetic markers. Within the ZINCAGE study, 819 healthy old Europeans (>or=60 years old) were recruited. Plasma zinc, interleukin-6 (IL-6) and interleukin-8 (IL-8) were measured. Genotype data were obtained for the -174G/C polymorphism in the IL-6 gene. Dietary data were collected with a food frequency questionnaire and were used to calculate a zinc diet score. Zinc score was validated using additional dietary data (24-h recalls), in a subsample of 105 subjects. Zinc score was different among most of the European centres (P<.001), while an age-dependent decline was documented (P=4.4x10(-12)). Plasma zinc concentrations were significantly correlated with the zinc score (standardized beta=0.144, P=8.8x10(-5)). The minor allele frequency for the -174G/C polymorphism was f(C) 0.31. There was a significant interaction of zinc diet score and GG (-174G/C) genotype on higher plasma IL-6 levels (beta+/-S.E.=0.014+/-0.0, P=.008). The main finding of our study was the detection of gene-nutrient and biochemical-nutrient interactions in a multiethnic cohort based on a common dietary assessment tool.
authors.affiliation: Department of Nutrition Science and Dietetics, Harokopio University of Athens, 17671 Kallithea-Athens, Greece.
authors.forename: Stavroula
authors.initials: S
authors.lastname: Kanoni
authors.forename: George V
authors.initials: GV
authors.lastname: Dedoussis
authors.forename: Georges
authors.initials: G
authors.lastname: Herbein
authors.forename: Tamas
authors.initials: T
authors.lastname: Fulop
authors.forename: Audrey
authors.initials: A
authors.lastname: Varin
authors.forename: Jolanta
authors.initials: J
authors.lastname: Jajte
authors.forename: Lothar
authors.initials: L
authors.lastname: Rink
authors.forename: Daniela
authors.initials: D
authors.lastname: Monti
authors.forename: Erminia
authors.initials: E
authors.lastname: Mariani
authors.forename: Marco
authors.initials: M
authors.lastname: Malavolta
authors.forename: Robertina
authors.initials: R
authors.lastname: Giacconi
authors.forename: Fiorella
authors.initials: F
authors.lastname: Marcellini
authors.forename: Eugenio
authors.initials: E
authors.lastname: Mocchegiani
mesh_terms: D000368:Aged; D000375:Aging; D015331:Cohort Studies; D004032:Diet; D019587:Dietary Supplements; D005006:Ethnicity; D005060:Europe; D005260:Female; D006801:Humans; D007249:Inflammation; D015850:Interleukin-6 / Q000378:metabolism; D016209:Interleukin-8 / Q000378:metabolism; D008297:Male; D052756:Nutritional Sciences; D011110:Polymorphism, Genetic; D014131:Trace Elements / Q000008:administration & dosage*; D015032:Zinc / Q000008:administration & dosage / Q000172:deficiency* / Q000627:therapeutic use*
chemical_list: D015850:Interleukin-6; D016209:Interleukin-8; D014131:Trace Elements; D015032:Zinc