title: Vaccination with Streptococcus suis serotype 2 recombinant 6PGD protein provides protection against S. suis infection in swine.
abstract: Streptococcus suis serotype 2 (S. suis 2 or SS2) is the causative agent of several diseases in both pigs and humans. 6-Phosphogluconate dehydrogenase (6PGD) is a cell surface protein in SS2. In this study, the immunogenicity and protective efficacy of recombinant 6PGD (r6PGD) from SS2 was evaluated in piglets. Immunization with an r6PGD-containing adjuvant induced a vigorous immunoglobulin G (IgG) response, with the titer of IgG2 being higher than that of IgG1. Immunization of piglets with r6PGD yielded 50% survival upon an intravenous challenge with a lethal dose of SS2. Piglets immunized with the r6PDG vaccine were better protected than those immunized with the adjuvant control. The clinical signs and histopathological changes in the piglets were recorded. Collectively, these results suggest that r6PGD can confer partial protection against SS2 infection and could be useful for the development of subunit vaccines against SS2.
authors.affiliation: State Key Laboratory of Agricultural Microbiology, Division of Animal Pathogens, College of Animal Science and Veterinary Medicine, Huazhong Agricultural University, Wuhan, China.
authors.forename: Chen
authors.initials: C
authors.lastname: Tan
authors.forename: Manli
authors.initials: M
authors.lastname: Liu
authors.forename: Jinlin
authors.initials: J
authors.lastname: Liu
authors.forename: Fangyan
authors.initials: F
authors.lastname: Yuan
authors.forename: Shulin
authors.initials: S
authors.lastname: Fu
authors.forename: Yuan
authors.initials: Y
authors.lastname: Liu
authors.forename: Meilin
authors.initials: M
authors.lastname: Jin
authors.forename: Weicheng
authors.initials: W
authors.lastname: Bei
authors.forename: Huanchun
authors.initials: H
authors.lastname: Chen
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D001426:Bacterial Proteins / Q000235:genetics / Q000276:immunology; D001921:Brain / Q000382:microbiology / Q000473:pathology; D005954:Glucosephosphate Dehydrogenase / Q000235:genetics / Q000276:immunology*; D007074:Immunoglobulin G / Q000097:blood; D008168:Lung / Q000382:microbiology / Q000473:pathology; D013290:Streptococcal Infections / Q000276:immunology / Q000517:prevention & control / Q000662:veterinary*; D022541:Streptococcal Vaccines / Q000008:administration & dosage / Q000276:immunology*; D017011:Streptococcus suis / Q000276:immunology*; D016019:Survival Analysis; D013552:Swine; D013553:Swine Diseases / Q000276:immunology / Q000517:prevention & control*; D022223:Vaccines, Subunit / Q000235:genetics / Q000276:immunology; D014614:Vaccines, Synthetic / Q000235:genetics / Q000276:immunology
chemical_list: D000907:Antibodies, Bacterial; D001426:Bacterial Proteins; D007074:Immunoglobulin G; D022541:Streptococcal Vaccines; D022223:Vaccines, Subunit; D014614:Vaccines, Synthetic; D005954:Glucosephosphate Dehydrogenase