Intracerebroventricular or intrathecal injection of glycine produces analgesia in thermal nociception and chemical nociception via glycine receptors.
The present study was designed to investigate the role of glycine receptors in analgesia induced by injection of glycine in vivo. Glycine was injected intracerebroventricularly or intrathecally and strychnine, a glycine receptor antagonist, was injected intracerebroventricularly or intrathecally before glycine injection. The effects on the pain threshold index in hot-plate test and the writhing times in acetic acid-induced writhing test were observed. The locomotor activity and motor performance (rotarod test) were also observed. The dosages of glycine and strychnine we choose had no effect on locomotor activity or motor performance in conscious mice. Glycine increased the pain threshold index in hot-plate test and decreased the writhing times of the mice. Strychnine antagonized the effects induced by glycine above. These results demonstrated that intracerebroventricular or intrathecal injection of glycine can produce analgesia in thermal nociception and chemical nociception in vivo, which is mediated by glycine receptors.
Cheng
Wei
W
Jiangsu Province Key Laboratory of Anesthesiology, Xuzhou Medical College, Affiliated Hospital of Xuzhou Medical College, Jiangsu Province, Xuzhou, Jiangsu, PR China.
Yin
Qin
Q
Cheng
Ming-Yue
MY
Chen
Hong-Sheng
HS
Wang
Shu
S
Feng
Tao
T
Zeng
Yin-Ming
YM
Liu
Gong-Jian
GJ
D019342:Acetic Acid / Q000009:adverse effects; D000698:Analgesia; D000700:Analgesics / Q000008:administration & dosage* / Q000494:pharmacology*; D000818:Animals; D001522:Behavior, Animal / Q000187:drug effects; D005260:Female; D005998:Glycine / Q000008:administration & dosage* / Q000494:pharmacology*; D006358:Hot Temperature / Q000009:adverse effects*; D007276:Injections, Intraventricular; D007278:Injections, Spinal; D008297:Male; D051379:Mice; D009043:Motor Activity / Q000187:drug effects; D010146:Pain / Q000139:chemically induced / Q000188:drug therapy* / Q000378:metabolism / Q000503:physiopathology; D017288:Pain Threshold / Q000187:drug effects; D018009:Receptors, Glycine / Q000037:antagonists & inhibitors / Q000378:metabolism*; D045442:Rotarod Performance Test; D013331:Strychnine / Q000008:administration & dosage / Q000494:pharmacology
D000700:Analgesics; D018009:Receptors, Glycine; D013331:Strychnine; D019342:Acetic Acid; D005998:Glycine