Comparative evaluation of hepatitis B surface antigen-loaded elastic liposomes and ethosomes for human dendritic cell uptake and immune response.
The aim of the present study was to evaluate two vesicular carrier systems, ethosomes and elastic liposomes loaded with hepatitis B surface antigen, for in vitro qualitative and quantitative uptake by human dendritic cells (DCs) and ability to stimulate T lymphocytes. Quantitative uptake of antigen-loaded carriers was documented by flow cytometry, and internalization of the systems by the DCs was studied using spectral bioimaging. Ability of antigen-pulsed DCs to stimulate autologous peripheral blood lymphocytes and levels of TH1/TH2 cytokines were also examined using flow cytometry. Both vesicular carrier systems as antigen delivery modules and DCs as antigen-presenting cells were able to generate a protective immune response. However, ethosomes were found to have higher internalizing ability and immunogenicity in comparison with elastic liposomes. These properties of ethosomes coupled with their skin-navigating potential, make it an attractive vehicle for development of a transcutaneous vaccine against hepatitis B in preference to elastic liposomes.


BACKGROUND
Two carrier systems for more potent vaccine administration - ethosomes and elastic liposomes loaded with hepatitis B surface antigen - are compared. Ethosomes demonstrated higher internalizing ability and immunogenicity. Due to their known skin-navigating potential, ethosomes may represent an attractive vehicle for development of a transcutaneous vaccine against hepatitis B.
Mishra
Dinesh
D
Department of Pharmaceutical Sciences, Dr. Hari Singh Gour University, Sagar, India. dineshdops@yahoo.com
Mishra
Pradyumna Kumar
PK
Dabadghao
Sunil
S
Dubey
Vaibhav
V
Nahar
Manoj
M
Jain
Narendra K
NK
D000818:Animals; D017209:Apoptosis; D001973:Bromodeoxyuridine / Q000378:metabolism; D002417:Cattle; D002467:Cell Nucleus / Q000378:metabolism; D049109:Cell Proliferation; D016207:Cytokines / Q000378:metabolism; D003713:Dendritic Cells / Q000276:immunology*; D004337:Drug Carriers / Q000737:chemistry*; D004548:Elasticity; D005434:Flow Cytometry; D016650:Fluorescein-5-isothiocyanate / Q000378:metabolism; D006514:Hepatitis B Surface Antigens / Q000276:immunology*; D006801:Humans; D007211:Indoles / Q000378:metabolism; D007700:Kinetics; D008081:Liposomes / Q000737:chemistry*; D051379:Mice; D041681:NIH 3T3 Cells; D009336:Necrosis; D012235:Rhodamines / Q000378:metabolism; D012710:Serum Albumin, Bovine / Q000378:metabolism; D018417:Th1 Cells / Q000166:cytology / Q000378:metabolism; D018418:Th2 Cells / Q000166:cytology / Q000378:metabolism; D013997:Time Factors
D016207:Cytokines; D004337:Drug Carriers; D006514:Hepatitis B Surface Antigens; D007211:Indoles; D008081:Liposomes; D012235:Rhodamines; D012710:Serum Albumin, Bovine; C007293:DAPI; D001973:Bromodeoxyuridine; D016650:Fluorescein-5-isothiocyanate