Quantum dot triexciton imaging with three-dimensional subdiffraction resolution.
We describe a simple method that improves optical resolution in fluorescence microscopy approximately 1.7-fold in all three dimensions and can be implemented on any basic confocal scanning microscope. This approach is based on three-photon absorption of commercially available quantum dots generating a triple exciton (triexciton) and subsequent blue-shifted fluorescence emission following recombination of the triexciton. As a pure physical approach, the resolution enhancement is independent from the nanoenvironment and demonstrated to work in living cells.
Hennig
Simon
S
Applied Laser Physics and Laser Spectroscopy and Bielefeld Institute for Biophysics and Nanoscience, Bielefeld University, Universitatsstrasse 25, 33615 Bielefeld, Germany.
van de Linde
Sebastian
S
Heilemann
Mike
M
Sauer
Markus
M