Role of toll-like receptor 3, RIG-I, and MDA5 in the expression of mesothelial IL-8 induced by viral RNA.
Interleukin-8 (IL-8) is a chemokine that has been shown to be a potent chemoattractant for polymorphonuclear neutrophils from the vascular compartment into the pleural space during infectious pleural effusions. Mesothelial cells express the viral receptors Toll-like receptor 3 (TLR3), RIG-I, and MDA5. Activation of these receptors by viral RNA exemplified by poly (I:C) RNA leads to a time- and dose-dependent increase of mesothelial IL-8 synthesis. To show the specific effect of viral receptors, knockdown experiments with short interfering RNA specific for TLR3, RIG-I and MDA5 were performed. This novel finding of functional expression of these viral sensors on human mesothelial cells may indicate a novel link between viral infections and mesothelial inflammation and indicates a pathophysiologic role of viral receptors in these processes.
Wörnle
Markus
M
Medical Policlinic, Ludwig-Maximilians-University, Pettenkoferstrasse 8a, 80336 Munich, Germany. Markus.Woernle@med.uni-muenchen.de
Sauter
Matthias
M
Kastenmüller
Kathrin
K
Ribeiro
Andrea
A
Mussack
Thomas
T
Ladurner
Roland
R
Sitter
Thomas
T
D002478:Cells, Cultured; D000071457:DEAD Box Protein 58; D053487:DEAD-box RNA Helicases / Q000502:physiology*; D004847:Epithelial Cells / Q000187:drug effects / Q000378:metabolism*; D006801:Humans; D000072640:Interferon-Induced Helicase, IFIH1; D016209:Interleukin-8 / Q000096:biosynthesis* / Q000235:genetics; D011070:Poly I-C / Q000494:pharmacology*; D012367:RNA, Viral / Q000494:pharmacology*; D011971:Receptors, Immunologic; D051196:Toll-Like Receptor 3 / Q000502:physiology*
D016209:Interleukin-8; D012367:RNA, Viral; D011971:Receptors, Immunologic; C495344:TLR3 protein, human; D051196:Toll-Like Receptor 3; C452820:RIGI protein, human; C452815:IFIH1 protein, human; D000071457:DEAD Box Protein 58; D053487:DEAD-box RNA Helicases; D000072640:Interferon-Induced Helicase, IFIH1; D011070:Poly I-C