title: Influence of low doses of deoxynivalenol on histopathology of selected organs of pigs.
abstract: Deoxynivalenol is one of mycotoxins that are most frequently determined in animal feed manufactured in Poland. The examination of histopathological lesions concomitant with deoxynivalenol intoxication is difficult because of the common, often synergistic, reaction of this mycotoxin with other toxins, such as zearalenone or ochratoxin A, which has a strong nephrotoxic activity. The possibility of estimating histopathological lesions in the course of intoxication with pure toxin at various doses is therefore of interest. Dosages used in this experiment relate to clinical cases observed in feeding the animals with whole ration feed obtained by processing feedingstuffs contaminated with Fusarium moulds. However, concerning the fact of one-shot administration of clinically pure toxin, the main question was if it was a sufficient dose to cause changes in the histopathological picture of gastrointestinal tract organs. The experiment was carried out on 12 nursery pigs of mixed breed (Polish White Large x Polish White Ear-pendent) with an average body weigh of 35 kg. The experimental nursery pigs were divided into 3 groups: group I (n=4)--control; group II (n=4)--DON administered at a dose of 0.2 mg/kg b.w.; group III (n=4)--DON administered at a dose of 0.4 mg/kg b.w. After slaughter of the animals, macroscopic examination was performed and segments of duodenum, jejunum, ileum, liver and mesenteric lymph nodes were sampled and assigned for histopathological examination. The results obtained equate to the clinically observed signs in swine production involving some nutrient metabolism disturbances in the gastrointestinal tract in the course of deoxynivalenol mycotoxicosis. Histopathological examination of segments of the duodenum, the jejunum, the ileum, the liver and the lymph nodes indicate that the regressive lesions are more expressed in the experimental group treated with the highest concentration of deoxynivalenol.
authors.affiliation: Division of Veterinary Prevention and Feed Hygiene, Department of Veterinary Public Health Protection, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, 10-718 Olsztyn, Poland. lukasz.zielonka@uwm.edu.pl
authors.forename: Ł
authors.initials: Ł
authors.lastname: Zielonka
authors.forename: M
authors.initials: M
authors.lastname: Wiśniewska
authors.forename: M
authors.initials: M
authors.lastname: Gajecka
authors.forename: K
authors.initials: K
authors.lastname: Obremski
authors.forename: M
authors.initials: M
authors.lastname: Gajecki
mesh_terms: D000818:Animals; D004305:Dose-Response Relationship, Drug; D005767:Gastrointestinal Diseases / Q000139:chemically induced / Q000473:pathology / Q000662:veterinary*; D007422:Intestines / Q000473:pathology; D008099:Liver / Q000473:pathology; D008198:Lymph Nodes / Q000473:pathology; D013552:Swine; D013553:Swine Diseases / Q000139:chemically induced* / Q000473:pathology; D014255:Trichothecenes / Q000008:administration & dosage* / Q000633:toxicity*
chemical_list: D014255:Trichothecenes; C007262:deoxynivalenol