title: Investigation of TLR5 and TLR7 as candidate genes for susceptibility to systemic lupus erythematosus.
abstract: OBJECTIVE
  The aim of this study was to evaluate the relevance of genetic variants of TLR5 (rs5744168) and TLR7 (rs179008) gene in systemic lupus erythematosus (SLE) in a Spanish population.
  
  
  METHODS
  Our study population consisted of 752 SLE patients and 1107 healthy controls. All individual were of Spanish Caucasian origin. The TLR5 and TLR7 polymorphisms were genotyped using a PCR system with pre-developed TaqMan allelic discrimination assay.
  
  
  RESULTS
  No statistically significant differences were observed when the allele and genotype distribution of TLR5 rs5744168 and TLR7 rs179008 polymorphisms was compared between SLE patients and healthy controls. A significant increase frequency in the CC genotype of the TLR5 rs5744168 polymorphism among SLE patients without nephritis was found (93% vs. 87% in SLE patients with nephritis, p=0.03, OR=2.11 95%CI 0.93-3.51). However, this difference did not reach statistical significance in the allele frequencies (p=0.08).
  
  
  CONCLUSIONS
  These results suggest that the tested variations of TLR5 and TLR7 genes do not confer a relevant role in the susceptibility or severity to SLE in the Spanish population.
authors.affiliation: Instituto de Parasitología y Biomedicina López-Neyra, CSIC, Granada, Spain. elena@ipb.csic.es
authors.forename: E
authors.initials: E
authors.lastname: Sánchez
authors.forename: J L
authors.initials: JL
authors.lastname: Callejas-Rubio
authors.forename: J M
authors.initials: JM
authors.lastname: Sabio
authors.forename: M A
authors.initials: MA
authors.lastname: Gónzalez-Gay
authors.forename: J
authors.initials: J
authors.lastname: Jimenez-Alonso
authors.forename: L
authors.initials: L
authors.lastname: Micó
authors.forename: E
authors.initials: E
authors.lastname: de Ramón
authors.forename: M
authors.initials: M
authors.lastname: Camps
authors.forename: A
authors.initials: A
authors.lastname: Suarez
authors.forename: C
authors.initials: C
authors.lastname: Gutierrez
authors.forename: R
authors.initials: R
authors.lastname: Garcia-Portales
authors.forename: C
authors.initials: C
authors.lastname: Tolosa
authors.forename: N
authors.initials: N
authors.lastname: Ortego-Centeno
authors.forename: J
authors.initials: J
authors.lastname: Sánchez-Román
authors.forename: F J
authors.initials: FJ
authors.lastname: Garcia-Hernández
authors.forename: M F
authors.initials: MF
authors.lastname: Gónzalez-Escribano
authors.forename: J
authors.initials: J
authors.lastname: Martin
mesh_terms: D016022:Case-Control Studies; D020022:Genetic Predisposition to Disease; D006801:Humans; D008180:Lupus Erythematosus, Systemic / Q000235:genetics*; D016017:Odds Ratio; D020641:Polymorphism, Single Nucleotide; D051198:Toll-Like Receptor 5 / Q000235:genetics*; D051199:Toll-Like Receptor 7 / Q000235:genetics*; D044465:White People
chemical_list: C495346:TLR5 protein, human; C495348:TLR7 protein, human; D051198:Toll-Like Receptor 5; D051199:Toll-Like Receptor 7