Improved health-relevant functionality in dark germinated Mucuna pruriens sprouts by elicitation with peptide and phytochemical elicitors.
The health-relevant functionality of Mucuna pruriens was improved by priming the seeds with elicitors of the pentose phosphate pathway (PPP) such as fish protein hydrolysates (FPHs), lactoferrin (LF) and oregano extract (OE) followed by dark germination. FPH elicited the highest phenolic content of 19 mg/g FW on day 1, which was 38% higher than control sprouts. OE enhanced Parkinson's disease-relevant L-DOPA content by 33% on day 1 compared to control sprouts. Anti-diabetes-relevant alpha-amylase inhibition percent (AIP) and alpha-glucosidase inhibition percent (GIP) were high in the cotyledons and decreased following elicitation and sprouting. For potential anti-diabetic applications, low AIP and high GIP with moderate L-DOPA content on day 4 of dark germination could be optimal. Improved L-DOPA concentrations in a soluble phenolic and antioxidant-rich M. pruriens background on day 1 sprouts have potential for Parkinson's disease management.
Randhir
Reena
R
Department of Food Science, Chenoweth Laboratory, University of Massachusetts, Amherst, MA 01003, USA.
Kwon
Young-In
YI
Shetty
Kalidas
K
D000975:Antioxidants / Q000378:metabolism; D003624:Darkness; D004791:Enzyme Inhibitors / Q000494:pharmacology; D029941:Fish Proteins / Q000494:pharmacology; D018525:Germination / Q000187:drug effects / Q000502:physiology*; D005954:Glucosephosphate Dehydrogenase / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000378:metabolism; D006262:Health; D007781:Lactoferrin / Q000494:pharmacology; D007980:Levodopa / Q000378:metabolism; D031299:Mucuna / Q000187:drug effects* / Q000201:enzymology; D031341:Origanum / Q000737:chemistry; D010427:Pentose Phosphate Pathway / Q000187:drug effects; D010455:Peptides / Q000494:pharmacology*; D010636:Phenols / Q000378:metabolism; D010936:Plant Extracts / Q000494:pharmacology*; D036226:Seedlings / Q000187:drug effects* / Q000201:enzymology; D013482:Superoxide Dismutase / Q000378:metabolism; D000516:alpha-Amylases / Q000037:antagonists & inhibitors
D000975:Antioxidants; D004791:Enzyme Inhibitors; D029941:Fish Proteins; D010455:Peptides; D010636:Phenols; D010936:Plant Extracts; D007980:Levodopa; D005954:Glucosephosphate Dehydrogenase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D000516:alpha-Amylases; D007781:Lactoferrin