title: Alternatively activated and immunoregulatory monocytes in human filarial infections.
abstract: BACKGROUND
  Monocytes/macrophages from filaria-infected animals exhibit an alternatively activated phenotype; however, very little is known about the alternative activation phenotype of monocytes in human filarial infection.
  
  
  METHODS
  To elucidate the activation and cytokine profile of monocytes in human filarial infection, we examined the expression patterns of genes encoding arginase, nitric oxide synthase 2, alternative activation markers, and cytokines in monocytes from individuals with asymptomatic filarial infection and individuals without filarial infection, ex vivo and in response to filarial antigen (Brugia malayi antigen [BmA]).
  
  
  RESULTS
  Monocytes from patients with asymptomatic filarial infection exhibited significantly diminished expression of NOS2 and significantly enhanced expression of ARG1. These changes were associated with significantly increased expression of the genes encoding resistin, mannose receptor C type 1 (MRC1), macrophage galactose type C lectin (MGL), and chemokine ligand 18 (CCL18). In response to BmA, purified monocytes from infected individuals also expressed significantly lower levels of interleukin (IL)-12 and IL-18 but, in contrast, expressed significantly higher levels of transforming growth factor beta, IL-10, and suppressor of cytokine signaling 1 mRNA. Inhibition of arginase-1 resulted in significantly diminished expression of the genes encoding resistin, MRC1, MGL, and CCL18, as well as significantly enhanced expression of NOS2 and the genes encoding IL-12 and IL-18.
  
  
  CONCLUSIONS
  Patent human filarial infection is associated with the presence of monocytes characterized by an alternatively activated immunoregulatory phenotype.
authors.affiliation: National Institutes of Health-International Center for Excellence in Research, Chennai, India. sbabu@mail.nih.gov
authors.forename: Subash
authors.initials: S
authors.lastname: Babu
authors.forename: V
authors.initials: V
authors.lastname: Kumaraswami
authors.forename: Thomas B
authors.initials: TB
authors.lastname: Nutman
mesh_terms: D000818:Animals; D000947:Antigens, Helminth / Q000276:immunology; D001119:Arginase / Q000235:genetics / Q000378:metabolism; D017178:Brugia malayi / Q000276:immunology*; D015331:Cohort Studies; D003170:Complement Pathway, Alternative; D016207:Cytokines / Q000235:genetics / Q000378:metabolism; D015536:Down-Regulation; D005368:Filariasis / Q000276:immunology*; D005786:Gene Expression Regulation / Q000276:immunology; D006801:Humans; D009000:Monocytes / Q000145:classification* / Q000276:immunology*; D052247:Nitric Oxide Synthase Type II / Q000235:genetics / Q000378:metabolism; D015854:Up-Regulation
chemical_list: D000947:Antigens, Helminth; D016207:Cytokines; D052247:Nitric Oxide Synthase Type II; D001119:Arginase