[The correlation of myxovirus resistance 1 and 2'5'-ollgoadenylate synthetase 1 with clinical features of systemic lupus erythematosus].
OBJECTIVE
To investigate the expression of myxovirus resistance 1(MX1) and 2'5'-oligoadenylate synthetase 1(OAS1) genes in the peripheral blood leukocyte of patients with systemic lupus erythematosus (SLE), and to evaluated the relations between these genes expression levels and disease activity.


METHODS
In this study, there were 50 SLE petients, 20 non-SLE patients with rheumatic dieases, and 25 normal controls. The peripheral blood samples of these patients were collected, and the expression levels (indicated as delta Ct value) of MX1 and OAS1 were measured by Sybr green dye based real-time quantitative PCR method.


RESULTS
The deltaCt value of MX1 expression in SLE patients was 3.55 +/- 1.39, which was significantly higher than those of non-SLE patients (2.31 +/- 0.52, P = 0.000) and normal controls (2.23 +/- 1.05, P = 0.000). (2) The deltaCt value of OAS1 expression in SLE patients was 4.45 +/- 1.56, which also was significantly higher than those of non-SLE patients (3.03 +/- 0.76, P = 0.000) and normal controls (2.75 +/- 0.64, P = 0.000). (3) The deltaCt value of OAS1 was correlated with the SLEDAI scores (r = 0.338, P = 0.019) and serum IgA level. (4) The ACt value of MX1 was not correlated with the SLEDAI scores (r = 0.064, P = 0.661), but correlated with the serum levels of triglyceride (TG), cholesterol (TC), low density lipoprotein (LDL), albuminuria of 24 hours (r = 0.428, P = 0.003 r = 0.383, P = 0.009 r = 0.394, P = 0.007; r = 0.316, P = 0.025); (5) The deltaCt values of MX1 and OAS1 in the SLE patients with arthritis were significantly higher than those in non-arthritis SLE patients (3.04 +/- 1.42, P = 0.004; 3.89 +/- 1.49, P = 0.006).


CONCLUSIONS
The expression levels of both MX1 and OAS1 in SLE patients are up-regulated, the expression levels of OAS1 genes are associated with SLE disease activity. As IFN-induced genes, MX1 and OAS1 play their respective role in SLE.
Li
Min
M
Department of Rheumatology, West China Hospital, Sichuan University, Chengdu 610041, China.
Yang
Nan-ping
NP
Huang
Xiang-yang
XY
Zhao
Zhen-yi
ZY
Zhang
Jie
J
Liu
Xiao-xiao
XX
Pu
Xiao-ying
XY
Dong
Jian-ling
JL
Ye
Yang
Y
D015088:2',5'-Oligoadenylate Synthetase / Q000235:genetics / Q000378:metabolism*; D000293:Adolescent; D000328:Adult; D005260:Female; D019204:GTP-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D008180:Lupus Erythematosus, Systemic / Q000235:genetics* / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D064547:Myxovirus Resistance Proteins; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D015854:Up-Regulation; D055815:Young Adult
D064547:Myxovirus Resistance Proteins; D012333:RNA, Messenger; C475085:OAS1 protein, human; D015088:2',5'-Oligoadenylate Synthetase; D019204:GTP-Binding Proteins