title: Impression cytology features of conjunctival nevi reported as more noticeable.
abstract: OBJECTIVE
  To report the impression cytology features of conjunctival nevi reported as more noticeable.
  
  
  METHODS
  35 patients who reported that a conjunctival lesion had become more noticeable after color or size change were enrolled. On slit-lamp examination, a clinical diagnosis of nevus was made and lesions underwent impression cytology using acetate cellulose strips and a combined staining with PAS, H&E and Papanicolaou. At patient's or parents' request, excision of the lesion was performed and the tissue was submitted to histopathological study.
  
  
  RESULTS
  Impression cytology examination revealed nests or cluster of nevus cells within the epithelium layer containing or not mucous-secreting goblet cells in 32 cases (91.4%). Ten patients (28.5%) had the tumor removed and histopathological diagnosis was compound nevus in 8 eyes (1 from caruncle, 1 from plica semilunaris and 6 from bulbar conjunctiva) and subepithelial nevus from bulbar conjunctiva (2 eyes).
  
  
  CONCLUSIONS
  Optical microscopy analysis of the impression cytology specimens confirmed the clinical diagnosis by demonstrating typical histopathological features of the superficial layers from conjunctival nevi in 91.4% of the cases. For amelanotic nevi IC can also allow a differential diagnosis from other nonpigmented lesions. The technique does not replace histopathological examination, but additionally, may assist in evaluating nevus cells in children and adults.
authors.affiliation: Departamento de Oftalmologia, Instituto da Visão, Universidade Federal de São Paulo, SP, Brasil. jeisonbarros@hotmail.com
authors.forename: Jeison de Nadai
authors.initials: Jde N
authors.lastname: Barros
authors.forename: Márcia Serva
authors.initials: MS
authors.lastname: Lowen
authors.forename: Vera Lucia Degaspare Monte
authors.initials: VL
authors.lastname: Mascaro
authors.forename: Thais Piedade de
authors.initials: TP
authors.lastname: Andrade
authors.forename: Maria Cristina
authors.initials: MC
authors.lastname: Martins
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D002648:Child; D002675:Child, Preschool; D003230:Conjunctival Neoplasms / Q000473:pathology* / Q000601:surgery; D003430:Cross-Sectional Studies; D003941:Diagnostic Techniques, Ophthalmological; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009506:Nevus / Q000473:pathology* / Q000601:surgery; D055815:Young Adult