Binding sites of valsartan, candesartan and losartan with angiotensin II receptor 1 subtype by molecular modeling.
OBJECTIVE
This study was designed to examine the importance of interaction in the binding of selective angiotensin II receptor antagonists to angiotensin II type 1 receptor using molecular modeling. The AT(1) antagonists used in this study were valsartan, candesartan and losartan.


METHODS
AT(1) receptor structural model was constructed by homology modeling using structural models of rhodopsin photointermediates. Through molecular modeling, possible binding sites for these drugs were suggested to lie between transmembrane domains (TM) 3, 5, and 6 of AT(1) receptor.


RESULTS
The carboxylic acid group and tetrazole ring of valsartan possibly interact with Lys199 of TM5 and Ser109 of TM3 and Asn295 of TM7 of AT(1) receptor, respectively. In candesartan, carboxylic group, tetrazole ring, and ethoxy group oxygen possibly interact with Lys199 of TM5, Ser109 of TM3 and Asn295 of TM7 and Gln257 of TM6, respectively. In losartan, tetrazole ring and hydroxymethyl group possibly interact with Asn295 of TM7 and Ser109 of TM3, respectively.


CONCLUSIONS
The results of the present study suggested that candesartan interacts at a higher number of binding sites compared to valsartan whereas losartan has a lower number of binding sites with the amino acid residues of the AT(1) receptor. These findings are consistent with the data of the radioligand-binding studies of the antagonists with the AT(1) receptor.
Bhuiyan
Mohiuddin Ahmed
MA
Department of Pharmacology, Faculty of Pharmaceutical Sciences, Niigata University of Pharmacy and Applied Life Sciences, 265-1 Higashijima, Akihaku, Niigata-shi 956-8603, Japan.
Ishiguro
Masaji
M
Hossain
Murad
M
Nakamura
Takashi
T
Ozaki
Masanobu
M
Miura
Shin-Ichiro
S
Nagatomo
Takafumi
T
D047228:Angiotensin II Type 1 Receptor Blockers / Q000737:chemistry / Q000378:metabolism*; D001562:Benzimidazoles / Q000737:chemistry / Q000378:metabolism*; D001665:Binding Sites; D001713:Biphenyl Compounds; D002460:Cell Line; D006801:Humans; D019808:Losartan / Q000737:chemistry / Q000378:metabolism*; D008958:Models, Molecular; D011487:Protein Conformation; D011869:Radioligand Assay; D044140:Receptor, Angiotensin, Type 1 / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D013777:Tetrazoles / Q000737:chemistry / Q000378:metabolism*; D014633:Valine / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism; D000068756:Valsartan
D047228:Angiotensin II Type 1 Receptor Blockers; D001562:Benzimidazoles; D001713:Biphenyl Compounds; D044140:Receptor, Angiotensin, Type 1; D013777:Tetrazoles; D000068756:Valsartan; D014633:Valine; D019808:Losartan; C081643:candesartan