title: Parsley extract inhibits in vitro and ex vivo platelet aggregation and prolongs bleeding time in rats.
abstract: Many cardiovascular diseases are associated with an increase in blood platelet activity. In Morocco, parsley (Petroselinum crispum, Apiaceae) is one of the medicinal herbs used to treat cardiovascular diseases such as arterial hypertension. In this study, crude aqueous extract (CAE) of parsley was evaluated for its anti-platelet activity in experimental animals on platelet aggregation in vitro and ex vivo; and on bleeding time in vivo. The in vitro aggregation was monitored after pre-incubation of platelets with CAE. The bleeding time and ex vivo aggregation were performed after oral treatment. CAE inhibited dose dependently platelet aggregation in vitro induced by thrombin, ADP, collagen and epinephrine. The oral administration of CAE (3g/kg) inhibited significantly (p<0.001) platelet aggregation ex vivo and prolonged bleeding time (p<0.001) without changes in the platelet amount. The prolongation of bleeding time by CAE may be attributed to the observed inhibition of platelet aggregation. These effects could be related in part to the polyphenolic compounds present in the extract. These results support the hypothesis that the dietary intake of parsley may be benefit in the normalization of platelet hyperactivation, in the nutritional prevention of cardiovascular diseases and are potentially interesting in the development of new prevention strategies.
authors.affiliation: Université Mohamed I(er), Faculté des Sciences, Laboratoire de Physiologie et Ethnopharmacologie, UFR "Physiologie et Pharmacologie", BP717, Oujda, Morocco.
authors.forename: Dounia
authors.initials: D
authors.lastname: Gadi
authors.forename: Mohamed
authors.initials: M
authors.lastname: Bnouham
authors.forename: Mohammed
authors.initials: M
authors.lastname: Aziz
authors.forename: Abderrahim
authors.initials: A
authors.lastname: Ziyyat
authors.forename: Abdelkhaleq
authors.initials: A
authors.lastname: Legssyer
authors.forename: Chantal
authors.initials: C
authors.lastname: Legrand
authors.forename: Françoise Fauvel
authors.initials: FF
authors.lastname: Lafeve
authors.forename: Hassane
authors.initials: H
authors.lastname: Mekhfi
mesh_terms: D000818:Animals; D001777:Blood Coagulation / Q000187:drug effects*; D066298:In Vitro Techniques; D028528:Petroselinum / Q000737:chemistry*; D010936:Plant Extracts / Q000494:pharmacology*; D010975:Platelet Aggregation Inhibitors / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D010936:Plant Extracts; D010975:Platelet Aggregation Inhibitors