Structure of human Brn-5 transcription factor in complex with CRH gene promoter.
The Brn-5 protein, highly expressed in human brain, belongs to the POU family; a class of transcription factors involved in a wide variety of biological processes ranging from programming of embryonic stem cells to cellular housekeeping. This functional diversity is conferred by two DNA-binding subdomains that can assume several configurations due to a bipartite arrangement of POU-specific (POU(S)) and POU-homeo (POU(H)) subdomains separated by a linker region. The crystal structure of human Brn-5 transcription factor in complex with corticotrophin-releasing hormone (CRH) gene promoter reveals an unexpected recognition mode of the protein to its cognate DNA. Moreover, the structure also shows the role of the linker in allowing diverse configurations that can be assumed by the two subdomains.
Pereira
Jose Henrique
JH
Department of Chemistry, University of California, Berkeley, CA 94720, USA.
Kim
Sung-Hou
SH
D003346:Corticotropin-Releasing Hormone / Q000235:genetics*; D018360:Crystallography, X-Ray; D006801:Humans; D050809:POU Domain Factors / Q000737:chemistry*; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D011487:Protein Conformation; D014157:Transcription Factors
D050809:POU Domain Factors; C082703:POU6F1 protein, human; D014157:Transcription Factors; D003346:Corticotropin-Releasing Hormone