[Study on the fingerprint of processed Rhizoma Atractylodis Macrocephalae by HPLC].
OBJECTIVE
To establish HPLC fingerprint for the quality control of processed Rhizoma Atractylodis Macrocephalae (PRAM).


METHODS
14 batches of PRAM were collected from different places and were analyzed with the developed HPLC fingerprints method. The HPLC separation was performed on a Kromasil C18 analytical column (250 mm x 4.6 mm, 5 microm), and gradient elution was performed by mobile phase containing acetonitrile and water. The flow rate was 1.0 mL/min and the detection wavelength was at 242 nm. The temperature of column was 25 degrees C.


RESULTS
Sixteen mutual peaks were selected in chromatography. Among the obtained fingerprints, the most of the detected peaks were separated effectively. The methodological evaluation showed that the method had a good repeatability.


CONCLUSIONS
The RSD of relative retention time of mutual peaks which existed in all samples was less than 1.1%. The results of peak areas were in accordance with the request of fingerprint. The established fingerprint can be used for the quality control and species identifying of PRAM.
Chen
Qin-hua
QH
Department of Pharmacy, Affiliated Dongfeng Hospital, Yunyang Medical College, Shiyan 442008, China. cqh77@163.com
Li
Peng
P
He
Jing
J
Zhu
Jun
J
Chen
Shu-yan
SY
D019659:Asteraceae / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D011786:Quality Control; D015203:Reproducibility of Results; D027343:Rhizome / Q000737:chemistry
D004365:Drugs, Chinese Herbal