[Diagnostics of genital herpesvirus infection in women with tubal-peritoneal infertility].
OBJECTIVE
Optimization of diagnostics of genital herpesvirus infection (herpes simplex virus types 1 and 2, cytomegalovirus [CMV]) in women with tubal-peritoneal infertility on the basis of detection of viral material in various parts of genital system using polymerase chain reaction (PCR) and revealing pathogenetically significant systemic abnormalitites of cytokine- and nitroxidergic regulation.


METHODS
Sixty-three women with tubal-peritoneal infertility were included in the study. Immunoenzyme assay, PCR, and modified Griess method were used.


RESULTS
In cervical canal herpesvirus infection was detected together with other agents in 30.1% of cases. In upper parts of reproductive system monoinfection with herpesvirus was detected in 74.63% of cases (herpes virus types 1 and 2--in 46.03%, CMV--in 28.6% of women). Increased levels of stable end metabolites of nitric oxide and cytokines were noted in sera of tested women.


CONCLUSIONS
Inflammatory changes in reproductive organs in women with tubal-peritoneal infertility are determined by chronic herpesvirus infection. This leads to Th1 polarization of immune response and developing of immune-mediated inflammation.
Medvedev
B I
BI
Teplova
S N
SN
Zaĭnetdinova
L F
LF
D000328:Adult; D002584:Cervix Uteri / Q000821:virology; D002908:Chronic Disease; D016207:Cytokines / Q000097:blood; D003587:Cytomegalovirus / Q000235:genetics / Q000302:isolation & purification; D003586:Cytomegalovirus Infections / Q000150:complications / Q000175:diagnosis; D004279:DNA, Viral / Q000032:analysis*; D005187:Fallopian Tubes / Q000821:virology; D005260:Female; D006558:Herpes Genitalis / Q000150:complications / Q000175:diagnosis* / Q000821:virology; D018259:Herpesvirus 1, Human / Q000235:genetics / Q000302:isolation & purification*; D018258:Herpesvirus 2, Human / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D007247:Infertility, Female / Q000097:blood / Q000821:virology*; D009569:Nitric Oxide / Q000097:blood / Q000378:metabolism
D016207:Cytokines; D004279:DNA, Viral; D009569:Nitric Oxide