Effect of bilayer phospholipid composition and curvature on ligand transfer by the alpha-tocopherol transfer protein.
We report here our preliminary investigations on the mechanism of alpha-TTP-mediated ligand transfer as assessed using fluorescence resonance energy transfer (FRET) assays. These assays monitor the movement of the model alpha-tocopherol fluorescent derivative ((R)-2,5,7,8-tetramethyl-chroman-2-[9-(7-nitro-benzo[1,2,5]oxadiazol-4-yl amino)-nonyl]-chroman-6-ol; NBD-Toc) from protein to acceptor vesicles containing the fluorescence quencher TRITC-PE. We have found that alpha-TTP utilizes a collisional mechanism of ligand transfer requiring direct protein-membrane contact, that rates of ligand transfer are greater to more highly curved lipid vesicles, and that such rates are insensitive to the presence of anionic phospholipids in the acceptor membrane. These results point to hydrophobic features of alpha-TTP dominating the binding energy between protein and membrane.
Zhang
Wen Xiao
WX
Department of Chemistry, Centre for Biotechnology, Brock University, 500 Glenridge Ave, St. Catharines, ON L2S 3A1, Canada.
Frahm
Grant
G
Morley
Samantha
S
Manor
Danny
D
Atkinson
Jeffrey
J
D001693:Biological Transport, Active / Q000187:drug effects; D002352:Carrier Proteins / Q000378:metabolism* / Q000502:physiology; D004734:Energy Metabolism; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D008024:Ligands; D008051:Lipid Bilayers / Q000737:chemistry*; D008081:Liposomes / Q000737:chemistry / Q000378:metabolism; D008560:Membrane Fluidity / Q000187:drug effects*; D010743:Phospholipids / Q000737:chemistry / Q000494:pharmacology*; D024502:alpha-Tocopherol / Q000378:metabolism* / Q000493:pharmacokinetics
D002352:Carrier Proteins; D008024:Ligands; D008051:Lipid Bilayers; D008081:Liposomes; D010743:Phospholipids; C017763:alpha-tocopherol transfer protein; D024502:alpha-Tocopherol