Velocity, processivity, and individual steps of single myosin V molecules in live cells.
We report the tracking of single myosin V molecules in their natural environment, the cell. Myosin V molecules, labeled with quantum dots, are introduced into the cytoplasm of living HeLa cells and their motion is recorded at the single molecule level with high spatial and temporal resolution. We perform an intracellular measurement of key parameters of this molecular transporter: velocity, processivity, step size, and dwell time. Our experiments bridge the gap between in vitro single molecule assays and the indirect measurements of the motor features deduced from the tracking of organelles in live cells.
Pierobon
Paolo
P
Institut Curie, Centre de Recherche, Laboratoire Physico-Chimie Curie, Centre National de la Recherche Scientifique, UMR 168, Université Pierre et Marie Curie-Paris, Paris, France.
Achouri
Sarra
S
Courty
Sébastien
S
Dunn
Alexander R
AR
Spudich
James A
JA
Dahan
Maxime
M
Cappello
Giovanni
G
D000818:Animals; D019921:Biotinylation; D002147:Calmodulin / Q000378:metabolism; D002470:Cell Survival; D003593:Cytoplasm / Q000378:metabolism; D005456:Fluorescent Dyes / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D042541:Intracellular Space / Q000378:metabolism; D009068:Movement; D024701:Myosin Type V / Q000032:analysis / Q000378:metabolism*; D021381:Protein Transport; D045663:Quantum Dots; D011817:Rabbits; D012666:Semiconductors; D013194:Staining and Labeling; D019809:Streptavidin / Q000378:metabolism; D013997:Time Factors
D002147:Calmodulin; D005456:Fluorescent Dyes; D019809:Streptavidin; D024701:Myosin Type V