[Experimental study on effect of total flavone of Ginkgo biloba on delaying cell senescence].
OBJECTIVE
To investigate the mechanism of delaying the senescence of human diploid fibroblast (2BS) by total flavone of Ginkgo biloba (FG).


METHODS
The drug sera of FG was used to treat the 2BS. The population doublings of 2BS cells were observed, the mRNA expression of P16 gene was determined by fluorescence real-time quantitative PCR.


RESULTS
FG significantly extended the population doublings of 2BS cells, and decreased the expression of P16 mRNA.


CONCLUSIONS
FG can delay the senescence of cells by inhibiting the P16 gene expression.
Song
Xiao-ping
XP
Department of Biology, Anhui Medical College, Hefei 230061, China. sxp@ahyz.cn
Chen
Zhi-wu
ZW
Fang
An-ning
AN
Fang
Pei-fei
PF
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D002453:Cell Cycle / Q000187:drug effects; D002478:Cells, Cultured; D016922:Cellular Senescence / Q000187:drug effects*; D019941:Cyclin-Dependent Kinase Inhibitor p16 / Q000378:metabolism*; D047309:Flavones / Q000494:pharmacology*; D005786:Gene Expression Regulation; D020441:Ginkgo biloba / Q000737:chemistry*; D006801:Humans; D008297:Male; D018515:Plant Leaves / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats
D000975:Antioxidants; D019941:Cyclin-Dependent Kinase Inhibitor p16; D047309:Flavones; D012333:RNA, Messenger