Chemical and biological characterization of siderophore  produced by the marine-derived Aureobasidium pullulans  HN6.2 and its antibacterial activity.
After analysis using HPLC and electronic ion spray mass spectroscopy, the purified siderophore produced by the marine-derived Aureobasidium pullulans HN6.2 was found to be fusigen. The purified desferric fusigen still had strong inhibition of growth of the pathogenic Vibrio anguillarum while the fusigen chelated by Fe3+ lost the ability to inhibit the growth of the pathogenic bacterium. The added iron in the medium repressed expression of the hydroxylase gene encoding ornithine N5-oxygenase that catalyzes the N5-hydroxylation of ornithine for the first step of siderophore biosynthesis in the yeast cells while expression of the hydroxylase gene in the yeast cells grown in the medium plus ornithine was enhanced.
Wang
Weilin
W
Key Laboratory of Marine Genetics and Gene Resource Exploitation (Ministry of Education), Ocean University of China, Yushan Road, No. 5, Qingdao, China.
Chi
Zhe
Z
Liu
Guanglei
G
Buzdar
Muhammad Aslam
MA
Chi
Zhenming
Z
Gu
Qianqun
Q
D000900:Anti-Bacterial Agents / Q000096:biosynthesis* / Q000737:chemistry*; D001203:Ascomycota / Q000235:genetics / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D005290:Ferric Compounds / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology*; D006877:Hydroxamic Acids / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology*; D006900:Hydroxylation; D007501:Iron / Q000378:metabolism; D013058:Mass Spectrometry; D006899:Mixed Function Oxygenases / Q000037:antagonists & inhibitors / Q000378:metabolism; D009952:Ornithine / Q000378:metabolism / Q000494:pharmacology; D012441:Saccharomyces cerevisiae / Q000378:metabolism; D017262:Siderophores / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics / Q000494:pharmacology*; D014733:Vibrio / Q000187:drug effects / Q000254:growth & development
D000900:Anti-Bacterial Agents; D005290:Ferric Compounds; D006877:Hydroxamic Acids; D017262:Siderophores; C010966:fusigen; D007501:Iron; D009952:Ornithine; D006899:Mixed Function Oxygenases; C059419:ornithine N5-oxygenase