[RP-HPLC determination of tiliroside in Daphne genkwa].
OBJECTIVE
To determine the contents of tiliroside [kaempferol 3-O-beta-D-(6 " -p-coumaroyl) glucopyranoside] in the Daphne genkwa.


METHODS
The separation was performed on ZORBAX XDB C18 column (4.6 mm x 250 mm, 5 microm), using acetonitrile and water/acetic acid mixture (1,000/1) (gradient elution: 0-18 min, 26 : 74; 18-33 min, 80 : 20) as mobile phase with the rate of 1.0 mL x min(-1) at 25 degrees C, the wavelength for measurement was 310 nm.


RESULTS
The linearity was in the range of 0.1-5.0 microg (n=6), and the correlation coefficient was 0.9999. The average recovery for compound tiliroside was 101.50% (RSD 0.90%) (n=6).


CONCLUSIONS
This method is accurate, reliable and reproducible to determine tiliroside in the D. genkwa.
Song
Lili
L
College of Chemistry of JiLin University, Changchun 130012, China.
Lu
Juan
J
Yan
Peifang
P
Tan
Lina
L
Li
Xuwen
X
Jin
Yongri
Y
D000097:Acetonitriles / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D032364:Daphne / Q000737:chemistry*; D005419:Flavonoids / Q000302:isolation & purification*; D009822:Oils, Volatile / Q000494:pharmacology; D018547:Plant Stems / Q000737:chemistry
D000097:Acetonitriles; C118943:Daphne repellent; D005419:Flavonoids; D009822:Oils, Volatile; C052083:tiliroside; C032159:acetonitrile