Sequential analysis of RNA synthesis by microchip electrophoresis.
We describe the potential of microchip electrophoresis with a Hitachi SV1100, which can be used to evaluate the integrity of total RNA, for the analysis of synthesized RNA. There was little interference by DNA and/or the components of the in vitro transcription system with the microchip electrophoresis. The fluorescence intensity corresponding to the synthesized RNA increased in a time-dependent manner as to the RNA synthesis reaction on sequential analysis. A result can be obtained in 160 s and only 1/10 aliquots of samples, compared with the conventional method, are required. These results indicate the potential of microchip electrophoresis for sequential analysis of RNA synthesis.
Umemoto
Yoshihiro
Y
Department of Molecular and Pharmaceutical Biotechnology, Graduate School of Pharmaceutical Sciences, University of Tokushima, Shomachi 1-78, Tokushima 770-8505, Japan.
Kataoka
Masatoshi
M
Yatsushiro
Shouki
S
Watanabe
Masahiro
M
Kido
Jun-Ichi
J
Kakuhata
Rei
R
Yamamoto
Takenori
T
Shinohara
Yasuo
Y
Baba
Yoshinobu
Y
D046209:Electrophoresis, Microchip / Q000295:instrumentation / Q000379:methods*; D005456:Fluorescent Dyes / Q000737:chemistry; D012313:RNA / Q000032:analysis / Q000096:biosynthesis*
D005456:Fluorescent Dyes; D012313:RNA