title: [Species characteristic and factors of persistence of gut bifidoflora during healthy state and dysbiosis].
abstract: OBJECTIVE
  To determine features of species composition and factors of persistence of bifidobacteria of human gut microflora in healthy state and during dysbiosis.
  
  
  METHODS
  Strains of bifidobacteria isolated from feces of patients with gut dysbiosis grades I - III (24 participants) and conditionally healthy persons (14 participants) were used for the study. Isolation and identification of microorganisms were performed by conventional methods. Antilysozyme activity was determined by photometric method and antilactoferrin sign--by immunoenzyme assay.
  
  
  RESULTS
  Bifidobacteria isolated from intestinal biotope have differences in species composition, which depended from age and state of microbiocenosis of studied persons. Bifidobacterium bifidum was dominated between strains of bifidobacteria isolated from infants with eubiosis, whereas B. longum dominated in persons > 1 y.o. B. bifidum and B. adolescentis were mainly isolated in infants with gut dysbiosis, where as in children > 1 y.o. B. adolescentis was predominantly isolated. New characteristic of bifidoflora--antilactoferrin activity--was discovered for the first time. Expression of this sign, as well as antilysozyme activity, varied and depended from species of bifidobacteria.
  
  
  CONCLUSIONS
  Obtained data widen our views on species composition of bifidobacteria and discover possible mechanisms of persistence of these microorganisms in intestinal biotope associated with inactivation of host defense factors--lysozyme and lactoferrin.
authors.forename: E V
authors.initials: EV
authors.lastname: Ivanova
authors.forename: N B
authors.initials: NB
authors.lastname: Perunova
authors.forename: A V
authors.initials: AV
authors.lastname: Valyshev
authors.forename: I V
authors.initials: IV
authors.lastname: Valysheva
authors.forename: O V
authors.initials: OV
authors.lastname: Bukharin
mesh_terms: D001644:Bifidobacterium / Q000145:classification* / Q000302:isolation & purification; D005243:Feces / Q000382:microbiology; D006801:Humans; D007223:Infant; D007410:Intestinal Diseases / Q000382:microbiology*; D007422:Intestines / Q000382:microbiology*; D007781:Lactoferrin / Q000032:analysis / Q000378:metabolism; D009113:Muramidase / Q000032:analysis / Q000378:metabolism
chemical_list: D009113:Muramidase; D007781:Lactoferrin