title: Identification of the human mature B cell miRNome.
abstract: The full set of microRNAs (miRNAs) in the human genome is not known. Because presently known miRNAs have been identified by virtue of their abundant expression in a few cell types, many tissue-specific miRNAs remain unrevealed. To understand the role of miRNAs in B cell function and lymphomagenesis, we generated short-RNA libraries from normal human B cells at different stages of development (naive, germinal center, memory) and from a Burkitt lymphoma cell line. A combination of cloning and computational analysis identified 178 miRNAs (miRNome) expressed in normal and/or transformed B cell libraries. Most notably, the B cell miRNome included 75 miRNAs which to our knowledge have not been previously reported and of which 66 have been validated by RNA blot and/or RT-PCR analyses. Numerous miRNAs were expressed in a stage- or transformation-specific fashion in B cells, suggesting specific functional or pathologic roles. These results provide a resource for studying the role of miRNAs in B cell development, immune function, and lymphomagenesis.
authors.affiliation: Institute of Cancer Genetics and Herbert Irving Comprehensive Cancer Center, Columbia University, New York, NY 10032, USA.
authors.forename: Katia
authors.initials: K
authors.lastname: Basso
authors.forename: Pavel
authors.initials: P
authors.lastname: Sumazin
authors.forename: Pavel
authors.initials: P
authors.lastname: Morozov
authors.forename: Christof
authors.initials: C
authors.lastname: Schneider
authors.forename: Roy L
authors.initials: RL
authors.lastname: Maute
authors.forename: Yukiko
authors.initials: Y
authors.lastname: Kitagawa
authors.forename: Jonathan
authors.initials: J
authors.lastname: Mandelbaum
authors.forename: Joseph
authors.initials: J
authors.lastname: Haddad
authors.forename: Chang-Zheng
authors.initials: CZ
authors.lastname: Chen
authors.forename: Andrea
authors.initials: A
authors.lastname: Califano
authors.forename: Riccardo
authors.initials: R
authors.lastname: Dalla-Favera
mesh_terms: D000818:Animals; D016175:B-Lymphocyte Subsets / Q000276:immunology*; D045744:Cell Line, Tumor; D004285:Dogs; D019143:Evolution, Molecular; D005786:Gene Expression Regulation; D000882:Haplorhini; D006801:Humans; D051379:Mice; D035683:MicroRNAs / Q000032:analysis / Q000235:genetics / Q000502:physiology*; D020411:Oligonucleotide Array Sequence Analysis; D014066:Palatine Tonsil / Q000378:metabolism; D051381:Rats
chemical_list: D035683:MicroRNAs