title: Design of high-density antibody microarrays for disease proteomics: key technological issues.
abstract: Antibody-based microarray is a novel proteomic technology setting a new standard for molecular profiling of non-fractionated complex proteomes. The first generation of antibody microarrays has already demonstrated its potential for generating detailed protein expression profiles, or protein atlases, of human body fluids in health and disease, paving the way for new discoveries within the field of disease proteomics. The process of designing highly miniaturized, high-density and high-performing antibody microarray set-ups have, however, proven to be challenging. In this mini-review we discuss key technological issues that must be addressed in a cross-disciplinary manner before true global proteome analysis can be performed using antibody microarrays.
authors.affiliation: Department of Immunotechnology, Lund University, Lund, Sweden.
authors.forename: Carl A K
authors.initials: CA
authors.lastname: Borrebaeck
authors.forename: Christer
authors.initials: C
authors.lastname: Wingren
mesh_terms: D001826:Body Fluids; D004247:DNA / Q000737:chemistry; D020869:Gene Expression Profiling; D006801:Humans; D007700:Kinetics; D013058:Mass Spectrometry / Q000379:methods; D040081:Protein Array Analysis / Q000379:methods*; D011506:Proteins / Q000737:chemistry; D020543:Proteome; D040901:Proteomics / Q000379:methods*; D020349:Surface Plasmon Resonance
chemical_list: D011506:Proteins; D020543:Proteome; D004247:DNA