In vivo osteogenic capability of human mesenchymal cells cultured on hydroxyapatite and on beta-tricalcium phosphate.
The aim of the current study was to examine in vitro osteogenic capability and in vivo bone formation of mesenchymal stromal cells (MSCs) on two kinds of calcium phosphate ceramics. MSCs derived from human bone marrow were seeded on either hydroxyapatite (HA) ceramic or beta-tricalcium phosphate (beta-TCP) ceramic and then cultured in a medium supplemented with a donor's serum, vitamin C, beta-glycerophosphate, and dexamethasone. The culture revealed the expression of alkaline phosphatase activity, indicating the osteogenic differentiation of the MSCs on the ceramics (fabrication of tissue-engineered construct). The constructs were then implanted subcutaneously into nude rats for 8 weeks. New bone formation was observed in both types of ceramics, and human-specific Alu sequence was detected by in situ hybridization analysis. Quantitative microcomputed tomography showed that the volume of the new bone in the HA ceramic was greater than that in the beta-TCP ceramic in six of seven cases. These results suggest that human MSCs cultured on ceramics could retain their osteogenic capability even after ectopic implantation and provide a rationale for the use of tissue-engineered constructs derived from a patient's MSCs and calcium phosphate ceramics in bone tissue regeneration.
Matsushima
Asako
A
Research Institute for Cell Engineering (RICE), National Institute of Advanced Industrial Science and Technology (AIST), Amagasaki, Hyogo, Japan.
Kotobuki
Noriko
N
Tadokoro
Mika
M
Kawate
Kenji
K
Yajima
Hiroshi
H
Takakura
Yoshinori
Y
Ohgushi
Hajime
H
D000328:Adult; D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology; D002130:Calcium Phosphates / Q000494:pharmacology*; D002454:Cell Differentiation / Q000187:drug effects; D003470:Culture Media / Q000737:chemistry; D017886:Durapatite / Q000494:pharmacology*; D005260:Female; D006801:Humans; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000187:drug effects*; D008875:Middle Aged; D010012:Osteogenesis / Q000187:drug effects*; D051381:Rats; D011923:Rats, Nude; D023822:Tissue Engineering / Q000379:methods*
D002130:Calcium Phosphates; D003470:Culture Media; C485817:beta-tricalcium phosphate; D017886:Durapatite