[Level of serum and liver tissue TGF-beta1 in patients with liver fibrosis due to chronic hepatitis B].
OBJECTIVE
To detect the level of serum and liver tissue TGF-beta1 in patients with chronic hepatitis B, to study their relation to liver fibrosis and gain the evidence for diagnosis of liver fibrosis.


METHODS
The liver fibrosis grades (S0-S4) of 131 cases with chronic HBV infection were diagnosed after liver biopsy. Serum TGF-beta1 was detected by enzyme-linked immunosorbent assay, and the semiquantitative analysis was applied after detecting the expression of TGF-beta1 in liver tissue with immunohistochemistry. Their relations to liver fibrosis were analyzed.


RESULTS
Serum and tissue level of TGF-beta1 increased significantly with the development of fibrosis, and the same result was obtained between themselves (P < 0.01). There was very significant difference for serum level of TGF-beta1 among the groups with different fibrosis grades (P < 0.01). Serum levels of TGF-beta1 were decreased significantly comparing the Group S0 or S1 to S4 (P < 0.005). There were significant difference for serum level of TGF-beta1 among S0 and the others (P < 0.005). And there was significant difference between S1 and S3 (P < 0.005). The expression level of TGF-beta1 in liver tissue has no significant difference between group S3 and S4 (P > 0.05). However, the differences were significantly among the other comparisons (P < 0.01).


CONCLUSIONS
There is close relation between the level of TGF-beta1 and the different liver fibrosis grades due to chronic hepatitis B. The serum level of TGF-beta1 is a potential noninvasive maker for diagnosis of liver fibrosis.
Guo
Jian-chun
JC
The Sixth People's Hospital of Hangzhou, Zhejiang 310014, China. guojianchun1961@126.com
Bao
Jian-feng
JF
Chen
Qun-wei
QW
Li
Xiao-ou
XO
Shi
Jun-ping
JP
Lou
Guo-qiang
GQ
Shi
Wei-zhen
WZ
Xun
Yun-hao
YH
D000328:Adult; D005260:Female; D006509:Hepatitis B / Q000150:complications* / Q000188:drug therapy / Q000378:metabolism / Q000473:pathology; D006515:Hepatitis B virus / Q000235:genetics / Q000378:metabolism*; D019694:Hepatitis B, Chronic / Q000150:complications* / Q000378:metabolism; D006801:Humans; D008103:Liver Cirrhosis / Q000209:etiology*; D008297:Male; D053773:Transforming Growth Factor beta1 / Q000097:blood / Q000378:metabolism*
D053773:Transforming Growth Factor beta1