[Effect of sequential intratumoral injection of xenogeneic antigens for immunotherapy in immunized mice bearing S180 tumor].
OBJECTIVE
To investigate the therapeutic effect of sequential intratumoral injection of xenogeneic antigens in immunized tumor-bearing mice.


METHODS
Sequential intratumoral injection of the xenoantigens was performed in immunized mice bearing S180 tumor. The tumor size changes were observed, and the tumor-infiltrating lymphocytes (TIL) including CD3+CD4+T, CD3+CD8+T, and CD3+CD4+CD25+T lymphocytes were counted with flow cytometry. The concentrations of IL-2 and TNF-alpha in the tumor was measured using ELISA.


RESULTS
No significant difference was found in the number of CD3+T lymphocytes in the TILs between different groups. After the immunotherapy, the percentages of CD3+CD4+T, CD3+CD8+T and CD3+CD4+CD25+T lymphocytes were 54%, 22% and 2.91%, respectively, with the CD4+/CD8+ ratio of 2.49, significantly different from that in the control group (P<0.05). The concentrations of IL-2 and TNF-alpha were 100.61 pg/ml and 54.114 pg/ml, respectively, significantly different from those in the control group (P<0.05).


CONCLUSIONS
Sequential intratumoral injection of heteragenetic antigena can significantly increase the amount of effector cells and cytokines in the micro-environment of the tumor, and decrease the expression of T regulatory.
Sun
Li-bin
LB
Oncology Center, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, China.
Zhang
Ji-ren
JR
Hu
Xi-gang
XG
Mao
Yan-tao
YT
D000818:Animals; D015478:Antigens, Heterophile / Q000276:immunology*; D016516:CD4-CD8 Ratio; D005260:Female; D007167:Immunotherapy / Q000379:methods*; D016246:Lymphocytes, Tumor-Infiltrating / Q000166:cytology*; D008297:Male; D051379:Mice; D011897:Random Allocation; D012510:Sarcoma 180 / Q000276:immunology / Q000628:therapy*; D013291:Streptococcus / Q000276:immunology*
D015478:Antigens, Heterophile