title: [HBV transinfected enhances the PD-L expression of cytokines stimulating].
abstract: OBJECTIVE
  To investigate whether the PD-L expression in the liver cell lines transinfected with HBV (HepG2.2.15 cells) can be up-regulated after cytokines stimulating.
  
  
  METHODS
  To apply the liver cell lines (HepG2 cells and HepG2.2.15 cells) as a model, the cells were stimulated with IL-4, IFN-alpha and IFN-gamma (final concentration were 10 ng/ml, stimulated for 12 hours) and RT-PCR was carried out to determine the PD-L expression before and after cytokines stimulating.
  
  
  RESULTS
  Whether or not transinfected with HBV, IFN-alpha and IFN-gamma both can induce the liver cell lines (HepG2 cells and HepG2.2.15 cells) PD-L1 expression while IL-4 can not; IL-4, IFN-alpha, IFN-gamma all can induce the PD-L2 expression in HepG2.2.15 cells which was transinfected with HBV, only IFN-gamma can induce the PD-L2 expression in HepG2 cells which was not transinfected with HBV.
  
  
  CONCLUSIONS
  IFN-alpha, IFN-gamma both can induce the PD-L1 expression in HepG2 cells and HepG2.2.15 cells, while it is easy for cytokines to induce the PD-L2 expression in HepG2.2.15 cells which was transinfected with HBV, this may provide a potential mechanism of the molecular basis for chronic HBV infection.
authors.affiliation: Department of Infectious Diseases, Peking University First Hospital, Beijing 100034, China.
authors.forename: Xue-jie
authors.initials: XJ
authors.lastname: Wu
authors.forename: Ji
authors.initials: J
authors.lastname: Chen
authors.forename: Man-hua
authors.initials: MH
authors.lastname: Zhu
authors.forename: Yuan
authors.initials: Y
authors.lastname: Hong
authors.forename: Lu
authors.initials: L
authors.lastname: Zhong
authors.forename: Gui-qiang
authors.initials: GQ
authors.lastname: Wang
mesh_terms: D045744:Cell Line, Tumor; D016207:Cytokines / Q000235:genetics / Q000378:metabolism*; D015870:Gene Expression; D006509:Hepatitis B / Q000378:metabolism* / Q000473:pathology; D006515:Hepatitis B virus; D022781:Hepatocytes / Q000378:metabolism* / Q000821:virology; D006801:Humans; D008099:Liver / Q000473:pathology*
chemical_list: D016207:Cytokines