title: A hyperdry amniotic membrane patch using a tissue adhesive for corneal perforations and bleb leaks.
abstract: OBJECTIVE
  To evaluate the efficacy of hyperdry amniotic membrane (AM) patching attached using a tissue adhesive for corneal perforations and glaucoma filtering bleb leaks.
  
  
  METHODS
  Prospective, noncomparative, interventional case series.
  
  
  METHODS
  Five eyes of 5 patients (glaucoma bleb leaks, 2 eyes; corneal perforations, 3 eyes) were treated with a single-layer patch of dried AM using a biological tissue adhesive. The dried AM was prepared with consecutive far-infrared rays and microwaves (hyperdry method) and was sterilized by gamma-ray irradiation. The dried AM was cut to the desired size and shape, and the tissue adhesive was applied to the amniotic epithelial side of the dried membrane. After applying the tissue adhesive, the dried membrane with glue applied then was positioned to cover the conjunctival bleb leak site or corneal perforation lesion using forceps. A therapeutic hydrogel contact lens then was installed as a bandage.
  
  
  RESULTS
  Bleb leaks or corneal perforations were repaired successfully within 21 days in all 5 cases. There were no remarkable adverse effects, and there was no recurrence of bleb leak or corneal perforation.
  
  
  CONCLUSIONS
  The hyperdry AM is a useful substrate, and this surgical procedure is a promising method to treat glaucoma filtering bleb leak or corneal perforation, which may result in serious vision-threatening ocular complications.
authors.affiliation: Department of Ophthalmology, University of Toyama, Graduate School of Medicine and Pharmaceutical Sciences Toyama, Japan.
authors.forename: Kiyotaka
authors.initials: K
authors.lastname: Kitagawa
authors.forename: Shuichiro
authors.initials: S
authors.lastname: Yanagisawa
authors.forename: Kazuhiko
authors.initials: K
authors.lastname: Watanabe
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Yunoki
authors.forename: Atsushi
authors.initials: A
authors.lastname: Hayashi
authors.forename: Motonori
authors.initials: M
authors.lastname: Okabe
authors.forename: Toshio
authors.initials: T
authors.lastname: Nikaido
mesh_terms: D000328:Adult; D000368:Aged; D000650:Amnion / Q000637:transplantation*; D001684:Biological Dressings; D001768:Blister / Q000601:surgery*; D003316:Corneal Diseases / Q000601:surgery*; D003487:Cyanoacrylates / Q000627:therapeutic use*; D003890:Desiccation; D005260:Female; D005500:Follow-Up Studies; D005902:Glaucoma, Open-Angle / Q000601:surgery*; D006801:Humans; D007429:Intraocular Pressure; D008297:Male; D008875:Middle Aged; D011446:Prospective Studies; D012422:Rupture, Spontaneous; D014014:Tissue Adhesives / Q000627:therapeutic use*; D014130:Trabeculectomy
chemical_list: D003487:Cyanoacrylates; D014014:Tissue Adhesives; C100832:octyl 2-cyanoacrylate