title: Establishment and characterization of a fibroblast cell line derived from Texel sheep.
abstract: A Texel sheep ear marginal tissue fibroblast cell line (named TSF19) was successfully established by using a primary explant technique and cell cryoconservation technology. TSF19 cells were adherent, with a population doubling time of 24.9 h. Chromosome analysis showed that >90% of cells were diploid prior to cell passage 4. Isoenzyme analyses of lactate dehydrogenase and malate dehydrogenase showed that the TSF19 cells had no cross-contamination with other species. Tests for cell line contamination with bacteria, fungi, or mycoplasmas were also negative. Plasmids encoding the fluorescent proteins pEGFP-N3, pECFP-N1, pDsRed1-N1, and pEYFP-N1 were transfected into cells to study exogenous gene expression in the cells. The plasmid transfection efficiency was between 21.8% and 46.5%. This newly established cell line will not only preserve the genetic resources of the important Texel sheep at the cell level but will also provide a valuable resource for genomic, postgenomic, somatic cloning research.
authors.affiliation: Institute of Animal Science, Chinese Academy of Agricultural Science, Beijing, China.
authors.forename: Linfeng F
authors.initials: LF
authors.lastname: Li
authors.forename: Weijun J
authors.initials: WJ
authors.lastname: Guan
authors.forename: Han
authors.initials: H
authors.lastname: Li
authors.forename: Xueyan Z
authors.initials: XZ
authors.lastname: Zhou
authors.forename: Xiujuan J
authors.initials: XJ
authors.lastname: Bai
authors.forename: Yuehui H
authors.initials: YH
authors.lastname: Ma
mesh_terms: D000818:Animals; D002455:Cell Division; D002460:Cell Line; D002874:Chromosome Mapping; D005347:Fibroblasts / Q000166:cytology*; D007621:Karyotyping; D010957:Plasmids; D012756:Sheep; D014162:Transfection