[Study of expression of TLR9, NF-kappaB, TNFalpha genes in cells of cervical canal mucosa in pregnant women with herpesvirus infection].
OBJECTIVE
To study changes in expression of TLR9, NF-kappaB, TNFalpha genes in cervical canal mucosa in pregnant women with herpesvirus infection.


METHODS
Using previously developed real-time polymerase chain reaction assays, levels of expression of genes, which corresponding proteins play important role in TLR9-dependent signaling pathway, was quantified.


RESULTS
Two-fold, 4.4-fold, and 3-fold rise in expression of TLR9, NF-kappaB, and TNFalpha genes respectively was revealed in pregnant women with herpesvirus infection compared to women with physiologically occurring pregnancy.


CONCLUSIONS
Obtained results demonstrated the activation of TLR-mediated mechanisms of innate immunity on the level of cervical canal mucosa during pregnancy complicated by viral infection.
Gankovskaia
O A
OA
Bakhareva
I V
IV
Gankovskaia
L V
LV
Somova
O Iu
OIu
Zverev
V V
VV
D000328:Adult; D002584:Cervix Uteri / Q000276:immunology* / Q000821:virology; D005260:Female; D015870:Gene Expression; D006566:Herpesviridae Infections / Q000235:genetics / Q000276:immunology*; D006801:Humans; D009092:Mucous Membrane / Q000276:immunology / Q000821:virology; D016328:NF-kappa B / Q000235:genetics*; D011247:Pregnancy; D011251:Pregnancy Complications, Infectious / Q000235:genetics / Q000276:immunology* / Q000821:virology; D051217:Toll-Like Receptor 9 / Q000235:genetics*; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics*; D015854:Up-Regulation
D016328:NF-kappa B; C495371:TLR9 protein, human; D051217:Toll-Like Receptor 9; D014409:Tumor Necrosis Factor-alpha