In vitro ovarian tumor growth and treatment response dynamics visualized with time-lapse OCT imaging.
In vitro three-dimensional models for metastatic ovarian cancer have been useful for recapitulating the human disease. These spheroidal tumor cultures, however, can grow in excess of 1 mm in diameter, which are difficult to visualize without suitable imaging technology.Optical coherence tomography (OCT) is an ideal live imaging method for non-perturbatively visualizing these complex systems. OCT enabled detailed observations of the model at both nodular and cellular levels, revealing growth dynamics not previously observed. The development of a time-lapse OCT system, capable of automated, multidimensional acquisition, further provided insights into the growth and chemotherapeutic response of ovarian cancer.
Evans
Conor L
CL
Wellman Center for Photomedicine, Massachusetts General Hospital, Harvard Medical School, 40 Blossom St, Boston, MA 02114, USA.
Rizvi
Imran
I
Hasan
Tayyaba
T
de Boer
Johannes F
JF
D045744:Cell Line, Tumor; D017076:Computer-Aided Design; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005260:Female; D006801:Humans; D007089:Image Enhancement / Q000295:instrumentation*; D018613:Microscopy, Confocal / Q000295:instrumentation*; D036641:Microscopy, Fluorescence, Multiphoton / Q000295:instrumentation*; D018715:Microscopy, Video / Q000295:instrumentation*; D010051:Ovarian Neoplasms / Q000473:pathology*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D041623:Tomography, Optical Coherence / Q000295:instrumentation*