title: Characterization of redox polymer based electrode and electrochemical behavior for DNA detection.
abstract: Characterization of redox polymer, poly(vinylferrocenium) perchlorate (PVF+ClO4-) coated as a film on Pt electrodes, and the detection of DNA based on the electrochemical behavior of the polymer were described in this study. PVF+ClO4- modified electrodes were prepared by the electrooxidation of poly(vinylferrocene) (PVF), and DNA immobilized polymer modified electrode was then prepared. The characterization of the polymer modified electrodes were performed by scanning tunneling microscopy (STM), Raman spectroscopy and alternating current (AC) impedance. The effects of DNA concentration, pH, and polymer films in various thicknesses based on the electrode response were also investigated. The electrochemical behavior of DNA modified polymer electrode by using double-stranded DNA (dsDNA) or single-stranded DNA (ssDNA) was compared. DNA hybridization was also investigated.
authors.affiliation: Department of Chemistry, Faculty of Science, Hacettepe University, 06532 Beytepe-Ankara, Turkey.
authors.forename: Filiz
authors.initials: F
authors.lastname: Kuralay
authors.forename: Arzum
authors.initials: A
authors.lastname: Erdem
authors.forename: Serdar
authors.initials: S
authors.lastname: Abaci
authors.forename: Haluk
authors.initials: H
authors.lastname: Ozyörük
authors.forename: Attila
authors.initials: A
authors.lastname: Yildiz
mesh_terms: D004247:DNA / Q000032:analysis*; D004563:Electrochemistry; D004566:Electrodes; D005296:Ferrous Compounds / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D008567:Membranes, Artificial; D016252:Microscopy, Scanning Tunneling; D010084:Oxidation-Reduction; D010984:Platinum / Q000737:chemistry*; D011145:Polyvinyls / Q000737:chemistry*; D013059:Spectrum Analysis, Raman
chemical_list: D005296:Ferrous Compounds; D008567:Membranes, Artificial; D011145:Polyvinyls; C451099:polyvinylferrocenium; D010984:Platinum; D004247:DNA