[Correlation of lymphoblastic PPAR-gamma mRNA expression with plasma IL-13 contents in children with acute idiopathic thrombocytopenic purpura].
OBJECTIVE
PPAR-gamma is associated with the differentiation, apoptosis, proliferation and cytokine secretion of immunologic cells. This study investigated peripheral blood lymphoblastic PPAR-gamma mRNA expression and its correlation with plasma IL-13 contents in children with acute idiopathic thrombocytopenic purpura (ITP).


METHODS
Fifty-three children with acute ITP who were in line with the standard test between September 2007 and July 2008 were enrolled. Fifty healthy children during the same period were used as the control group. PPAR-gamma mRNA expression in peripheral blood lymphocytes were detected by RT-PCR. Plasma IL-13 contents were detected using ELISA.


RESULTS
PPAR-gamma mRNA expression in peripheral blood lymphocytes from acute ITP children were significantly higher than that in the control group (0.78 +/- 0.03 vs 0.52 +/- 0.05; P< 0.05). Plasma IL-13 contents in children with acute ITP were also significantly higher than those in the control group (160.21 +/- 34.26 pg/mL vs 121.42 +/- 12.69 pg/mL; P< 0.05). There was a positive correlation between plasma IL-13 level and lymphoblastic PPAR-gamma mRNA expression in children with ITP (r=0.89, P< 0.05).


CONCLUSIONS
PPAR-gamma mRNA expression in peripheral blood lymphocytes increased and were positively correlated with plasma IL-13 contents in children with acute ITP, suggesting that PPAR-gamma and IL-13 might participate in the pathogenesis of acute ITP.
Jin
Cheng-Qiang
CQ
Department of Microbiology and Immunology, Guangdong Medical College, Zhanjiang, Guangdong, China.
Liu
Fang
F
Xiao
Hong
H
Wang
Wen-Juan
WJ
Chen
Qun
Q
Zheng
Bi-Ying
BY
Li
Guo-Ming
GM
D000208:Acute Disease; D002648:Child; D002675:Child, Preschool; D005260:Female; D006801:Humans; D018793:Interleukin-13 / Q000097:blood* / Q000502:physiology; D008214:Lymphocytes / Q000378:metabolism*; D008297:Male; D047495:PPAR gamma / Q000235:genetics* / Q000502:physiology; D016553:Purpura, Thrombocytopenic, Idiopathic / Q000209:etiology / Q000276:immunology*; D012333:RNA, Messenger / Q000032:analysis*
D018793:Interleukin-13; D047495:PPAR gamma; D012333:RNA, Messenger