Assessment of sensitivity and specificity of first, second, and third generation EIA for the detection of antibodies to HIV-1 in oral fluid.
The performances of three blood-based immunoassays test kits were compared with regard to their ability to detect HIV-1 antibody in oral fluid. It was found that these three kits differ in their ability to detect HIV-1 antibody. Notably, a third generation EIA which has been shown to possess superior sensitivity for antibody detection in plasma appears to possess no sensitivity advantage for detecting HIV-1 antibody in oral fluid.
Louie
Brian
B
San Francisco Department of Public Health Laboratory, 101 Grove Street, San Francisco, CA 94102, USA.
Lei
John
J
Liska
Sally
S
Dowling
Teri
T
Pandori
Mark W
MW
D000914:Antibodies, Viral / Q000097:blood / Q000276:immunology / Q000302:isolation & purification*; D015658:HIV Infections / Q000097:blood / Q000175:diagnosis* / Q000276:immunology; D015497:HIV-1 / Q000276:immunology*; D006801:Humans; D007124:Immunoenzyme Techniques / Q000379:methods*; D011933:Reagent Kits, Diagnostic; D012463:Saliva / Q000276:immunology*; D012680:Sensitivity and Specificity
D000914:Antibodies, Viral; D011933:Reagent Kits, Diagnostic