Bioavailability of salvianolic acid B and effect on blood viscosities after oral administration of salvianolic acids in beagle dogs.
Salvianolic acid B (SalB) is an active component isolated from Chinese herbal medicine Salvia miltiorrhiza. The aim of this study was to investigate the extent of absolute oral bioavailability (F) of SalB in beagle dogs and the effect on blood viscosity after intravenous and oral administration of Salvianolic acids (SAs). A gradient elution HPLC method was developed and validated to determine the concentration of SalB and its three possible metabolites in plasma. After SAs (180 mg/kg, p.o.; 9 mg/kg, i.v.) were given, the AUCs of SalB were 1680 +/- 670 and 7840 +/- 1140 ng/mL.h, respectively. The F of SalB in dogs was calculated to be only 1.07 +/- 0.43%. The blood viscosity was remarkably decreased after a single intravenous injection of SAs (9 mg/kg). However, no significant change of blood viscosity was observed after a single oral administration of SAs (180 mg/kg). The results suggested that the F of SalB was extremely low and single oral administrated SAs had no effect on ameliorating blood viscosity in beagle dogs.
Gao
Dong-Yan
DY
Department of Pharmaceutics, Fudan University, Shanghai, 201-203, People's Republic of China.
Han
Li-Mei
LM
Zhang
Li-Hong
LH
Fang
Xiao-Ling
XL
Wang
Jian-Xin
JX
D000284:Administration, Oral; D000818:Animals; D019540:Area Under Curve; D001572:Benzofurans / Q000008:administration & dosage* / Q000097:blood / Q000493:pharmacokinetics*; D001682:Biological Availability; D001711:Biotransformation; D001809:Blood Viscosity / Q000187:drug effects*; D002851:Chromatography, High Pressure Liquid; D004285:Dogs; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage* / Q000493:pharmacokinetics*; D018056:Hemorheology; D007275:Injections, Intravenous; D008297:Male; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet; D053630:Depsides
D001572:Benzofurans; D004365:Drugs, Chinese Herbal; C076944:salvianolic acid B; D053630:Depsides