title: Bifidogenic effect and stimulation of short chain fatty acid production in human faecal slurry cultures by oligosaccharides derived from lactose and lactulose.
abstract: Bifidogenic effect and stimulation of short chain fatty acid (SCFA) production by fractions of oligosaccharides with a DP> or =3 and Gal beta(1-6) linkages synthesised from lactose or lactulose by Pectinex Ultra SP-L and Lactozym 3000 L HP G were evaluated in human faecal slurries. Results were compared with those obtained for the commercial oligosaccharide mixture Vivinal-GOS. Quantitative real-time PCR showed that all galacto-oligosaccharide (GOS) fractions stimulated slightly higher bifidobacteria growth than lactose, lactulose and Vivinal-GOS. GOS fractions promoted the production of total SCFA and acetic acid in a similar way to Vivinal-GOS and more than glucose, lactose and lactulose. In conclusion, oligosaccharides derived from lactose and lactulose may represent a suitable alternative to lactulose for the design of new functional food ingredients.
authors.affiliation: Instituto de Fermentaciones Industriales (CSIC), Juan de la Cierva, 3, Madrid 28006, Spain.
authors.forename: Alejandra
authors.initials: A
authors.lastname: Cardelle-Cobas
authors.forename: María
authors.initials: M
authors.lastname: Fernández
authors.forename: Nuria
authors.initials: N
authors.lastname: Salazar
authors.forename: Cristina
authors.initials: C
authors.lastname: Martínez-Villaluenga
authors.forename: Mar
authors.initials: M
authors.lastname: Villamiel
authors.forename: Patricia
authors.initials: P
authors.lastname: Ruas-Madiedo
authors.forename: Clara G
authors.initials: CG
authors.lastname: de los Reyes-Gavilán
mesh_terms: D001644:Bifidobacterium / Q000187:drug effects / Q000235:genetics / Q000254:growth & development*; D002236:Carbohydrate Conformation; D004269:DNA, Bacterial / Q000032:analysis; D005227:Fatty Acids / Q000096:biosynthesis*; D005243:Feces / Q000382:microbiology*; D005285:Fermentation; D006801:Humans; D007785:Lactose / Q000378:metabolism*; D007792:Lactulose / Q000378:metabolism* / Q000494:pharmacology; D009844:Oligosaccharides / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D016133:Polymerase Chain Reaction; D019936:Probiotics; D013329:Structure-Activity Relationship
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D009844:Oligosaccharides; D007792:Lactulose; D007785:Lactose