title: Quantum dot triexciton imaging with three-dimensional subdiffraction resolution.
abstract: We describe a simple method that improves optical resolution in fluorescence microscopy approximately 1.7-fold in all three dimensions and can be implemented on any basic confocal scanning microscope. This approach is based on three-photon absorption of commercially available quantum dots generating a triple exciton (triexciton) and subsequent blue-shifted fluorescence emission following recombination of the triexciton. As a pure physical approach, the resolution enhancement is independent from the nanoenvironment and demonstrated to work in living cells.
authors.affiliation: Applied Laser Physics and Laser Spectroscopy and Bielefeld Institute for Biophysics and Nanoscience, Bielefeld University, Universitatsstrasse 25, 33615 Bielefeld, Germany.
authors.forename: Simon
authors.initials: S
authors.lastname: Hennig
authors.forename: Sebastian
authors.initials: S
authors.lastname: van de Linde
authors.forename: Mike
authors.initials: M
authors.lastname: Heilemann
authors.forename: Markus
authors.initials: M
authors.lastname: Sauer