An immunoaffinity column with a monoclonal antibody as ligand for human follicle stimulating hormone.
A detailed characterization regarding the chromatographic and biochemical properties of an immunoaffinity column for sample pretreatment using a follicle stimulating hormone (FSH) beta-unit specific mAb has been performed. A maximum binding capacity of approximately 300 microg/mL was determined. A K(D) value of 4.5x10(-8) M could be obtained in batch isotherm measurements. The dynamic binding capacity at 10% did not change when residence time was at least 6 min. High selectivity could be demonstrated using crude culture supernatant from FSH producing Chinese hamster ovary (CHO) cells. A great stability of the matrix was confirmed by performing 100 consecutive chromatographic runs without loss of capacity.
Zandian
Marlene
M
Department of Biotechnology, University of Applied Life Sciences and Natural Resources, Vienna, Austria.
Jungbauer
Alois
A
D000327:Adsorption; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D016466:CHO Cells; D002846:Chromatography, Affinity / Q000379:methods*; D002851:Chromatography, High Pressure Liquid; D006224:Cricetinae; D003412:Cricetulus; D005640:Follicle Stimulating Hormone / Q000032:analysis* / Q000276:immunology; D006801:Humans; D007700:Kinetics; D008024:Ligands
D000911:Antibodies, Monoclonal; D008024:Ligands; D005640:Follicle Stimulating Hormone