A study of the action of biological products on the living cell by measuring the oxygen consumption.
Summary The activity of a product applied locally to the skin may, in certain cases, cause an increase in the cell metabolism, reflected by an increase in oxygen consumption. The authors employed two methods involving epidermal cells of the rat and hepatic cells of the mouse, based on the polarographic measurement of oxygen pressures in the survival medium, using Clark's electrode. The first method mainly uses the epidermal cells of the rat. The investigation is carried out on a control group which is sufficiently large to be representative (50 animals). It yields the basic value of oxygen consumption of the cells in the survival liquid. In the test group, the epidermal cells from ten different animals consume oxygen in the presence of the test product.
Martini
M C
MC
Laboratoire de PharmacieIndustrielle et de Technologie Parapharmaceutique, Faculté de Pharmacie, 8, avenue Rockefeller, 69008 LYON, France.
Barraillier
S
S
Cotte
J
J