title: Leishmania (Viannia) peruviana (MHOM/PE/LCA08): comparison of THP-1 cell and murine macrophage susceptibility to axenic amastigotes for the screening of leishmanicidal compounds.
abstract: This study, undertaken to compare the susceptibility of THP-1 cells and murine peritoneal macrophages to Leishmania peruviana amastigotes, obtained THP-1 infection with 10 parasites/cell compared to 2 parasites/murine macrophage. The parasite burden was maximal at 72 h post-infection (h.p.i.) for THP-1 cells, while it was still increasing at 120 h.p.i. for murine macrophages. Since in both cases the infection with L. peruviana affected cell viability, we recommend evaluating any leishmanicidal activity at 72 h.p.i. Amphotericin B reduced Leishmania infection by 50% at concentrations of 0.1 microM in THP-1 and murine macrophages at 72 h.p.i. Our results demonstrate that amastigotes of L. peruviana can infect THP-1 cells and murine macrophages and indicate the suitability of this model to screen compounds for leishmanicidal activity.
authors.affiliation: Laboratorios de Investigación y Desarrollo, Facultad de Ciencias y Filosofía, Universidad Peruana Cayetano Heredia (UPCH), Av. Honorio Delgado 430, SMP, Lima, Peru.
authors.forename: Germán
authors.initials: G
authors.lastname: González
authors.forename: Denis
authors.initials: D
authors.lastname: Castillo
authors.forename: Yannick
authors.initials: Y
authors.lastname: Estevez
authors.forename: Thomas
authors.initials: T
authors.lastname: Grentzinger
authors.forename: Eric
authors.initials: E
authors.lastname: Deharo
mesh_terms: D000666:Amphotericin B / Q000494:pharmacology* / Q000633:toxicity; D000818:Animals; D000981:Antiprotozoal Agents / Q000494:pharmacology* / Q000633:toxicity; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D005260:Female; D006801:Humans; D020128:Inhibitory Concentration 50; D007892:Leishmania braziliensis / Q000187:drug effects / Q000472:pathogenicity*; D007948:Leukemia, Monocytic, Acute / Q000473:pathology; D017737:Macrophages, Peritoneal / Q000187:drug effects / Q000469:parasitology*; D051379:Mice; D009000:Monocytes / Q000187:drug effects / Q000469:parasitology*
chemical_list: D000981:Antiprotozoal Agents; D000666:Amphotericin B