Targeted gene delivery to the lung.
Gene therapy holds promise for the treatment of a range of inherited pulmonary disorders. However, efficient delivery and expression of the therapeutic transgene at levels sufficient to result in phenotypic correction of the diseased state has proved elusive. This review focuses on the development of gene delivery strategies for the lungs. One of the principal prerequisites for successful gene therapy is the delivery of gene vectors to the target area within a tissue and to target cells within that area. Physical and biological targeting of the gene vectors and its application in various models is discussed. Subsequently, both viral and non-viral vectors are addressed with respect to their transfection efficiency in different lung cells, the longevity of expression and their immunogenicity. Also, the various methods for pulmonary gene delivery are evaluated for their merits and limitations.
Aneja
Manish K
MK
Ludwig-Maximilians University, Division of Molecular Pulmonology, Department of Paediatrics, Lindwurmstrasse 2A, D-80337 Munich, Germany.
Geiger
Johannes-Peter
JP
Himmel
Anne
A
Rudolph
Carsten
C
D000818:Animals; D004279:DNA, Viral / Q000378:metabolism; D018014:Gene Transfer Techniques; D015316:Genetic Therapy / Q000379:methods*; D005822:Genetic Vectors; D006801:Humans; D008168:Lung; D008171:Lung Diseases / Q000628:therapy; D008954:Models, Biological; D020545:Respiratory Mucosa
D004279:DNA, Viral