The 30-kDa band from Salmonella typhimurium: IgM, IgA and IgG antibody response in patients with ankylosing spondylitis.
OBJECTIVE
To determine the association of Salmonella typhimurium antigens with AS by analysing the IgA, IgG and IgM antibody response to the crude lysate and the 30-kDa band from this micro-organism.


METHODS
Sera from 28 AS patients, 28 HLA-B27+ healthy relatives, 28 unrelated healthy subjects and 14 RA patients were included. Salmonella typhimurium proteins were electrophoretically separated and blotted onto nitrocellulose sheets for immunodetection with sera from AS patients and unrelated healthy subjects. The electroeluted 30-kDa band (p30) and a crude lysat (StCL) from S. typhimurium were used as antigen to evaluate the IgM, IgA and IgG (total and subclasses) antibody levels by ELISA. An inhibition assay was carried out to confirm the specificity of IgG response to the p30.


RESULTS
Twenty out of 28 AS patients (71.4%) and 4 out of 28 unrelated healthy subjects (14.3%) recognized a 30-kDa band from S. typhimurium with IgG antibodies. Six out of 28 AS patients (21.4%) and 4 out of 28 unrelated healthy subjects (14.3%) detected it with IgA antibodies. Recognition of p30 and StCL by both IgA and IgG antibodies was higher in AS patients than in control groups (P = 0.003, <0.001 and 0.003 for IgA and <0.001, 0.003 and 0.006 for IgG). Sera from AS patients have higher percentage of IgG antibodies p30 and IgG3 subclass was higher in AS patients than in control groups. No differences in the IgM response were found.


CONCLUSIONS
Data presented suggest the association between the p30 and AS.
Zambrano-Zaragoza
Jose F
JF
Laboratorio de Inmunología, Unidad Académica de Ciencias Químico Biológicas y Farmacéuticas, Universidad Autónoma de Nayarit, Tepic, Nayarit, México. jzambran@nayar.uan.mx
de Jesus Durán-Avelar
Ma
M
Rodríguez-Ocampo
Angelica N
AN
García-Latorre
Ethel
E
Burgos-Vargas
Ruben
R
Dominguez-Lopez
Maria-L
ML
Pena-Virgen
Salvador
S
Vibanco-Pérez
Norberto
N
D000328:Adult; D000818:Animals; D000917:Antibody Formation; D000942:Antigens, Bacterial / Q000302:isolation & purification / Q000494:pharmacology*; D001172:Arthritis, Rheumatoid / Q000276:immunology; D001426:Bacterial Proteins / Q000302:isolation & purification / Q000494:pharmacology; D015153:Blotting, Western / Q000379:methods; D016022:Case-Control Studies; D016009:Chi-Square Distribution; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D020022:Genetic Predisposition to Disease; D015796:HLA-B27 Antigen / Q000276:immunology; D006801:Humans; D007070:Immunoglobulin A / Q000276:immunology; D007074:Immunoglobulin G / Q000276:immunology; D007075:Immunoglobulin M / Q000276:immunology; D007136:Immunoglobulins / Q000032:analysis*; D008297:Male; D008875:Middle Aged; D012486:Salmonella typhimurium / Q000276:immunology*; D013167:Spondylitis, Ankylosing / Q000235:genetics / Q000276:immunology*; D055815:Young Adult
D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D015796:HLA-B27 Antigen; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D007136:Immunoglobulins