MHC class II-dependent basophil-CD4+ T cell interactions promote T(H)2 cytokine-dependent immunity.
Dendritic cells can prime naive CD4+ T cells; however, here we demonstrate that dendritic cell-mediated priming was insufficient for the development of T helper type 2 cell-dependent immunity. We identify basophils as a dominant cell population that coexpressed major histocompatibility complex class II and interleukin 4 message after helminth infection. Basophilia was promoted by thymic stromal lymphopoietin, and depletion of basophils impaired immunity to helminth infection. Basophils promoted antigen-specific CD4+ T cell proliferation and interleukin 4 production in vitro, and transfer of basophils augmented the population expansion of helminth-responsive CD4+ T cells in vivo. Collectively, our studies suggest that major histocompatibility complex class II-dependent interactions between basophils and CD4+ T cells promote T helper type 2 cytokine responses and immunity to helminth infection.
Perrigoue
Jacqueline G
JG
Department of Pathobiology, University of Pennsylvania, Philadelphia, Pennsylvania, USA.
Saenz
Steven A
SA
Siracusa
Mark C
MC
Allenspach
Eric J
EJ
Taylor
Betsy C
BC
Giacomin
Paul R
PR
Nair
Meera G
MG
Du
Yurong
Y
Zaph
Colby
C
van Rooijen
Nico
N
Comeau
Michael R
MR
Pearce
Edward J
EJ
Laufer
Terri M
TM
Artis
David
D
D000818:Animals; D001491:Basophils / Q000166:cytology / Q000276:immunology* / Q000378:metabolism; D039521:CD11c Antigen / Q000276:immunology; D015496:CD4-Positive T-Lymphocytes / Q000166:cytology / Q000276:immunology* / Q000378:metabolism; D002450:Cell Communication / Q000276:immunology; D049109:Cell Proliferation; D016207:Cytokines / Q000276:immunology* / Q000378:metabolism; D005260:Female; D005434:Flow Cytometry; D015870:Gene Expression; D000949:Histocompatibility Antigens Class II / Q000235:genetics / Q000276:immunology*; D007109:Immunity / Q000276:immunology*; D015151:Immunoblotting; D015847:Interleukin-4 / Q000235:genetics / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008815:Mice, Inbred Strains; D018345:Mice, Knockout; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012555:Schistosomiasis mansoni / Q000276:immunology / Q000469:parasitology; D018418:Th2 Cells / Q000378:metabolism; D013950:Thymus Gland / Q000166:cytology / Q000276:immunology; D014257:Trichuriasis / Q000276:immunology / Q000469:parasitology
D039521:CD11c Antigen; D016207:Cytokines; D000949:Histocompatibility Antigens Class II; D015847:Interleukin-4