title: [Effect of a novel fibrinolytic enzyme FA-I on thrombosis and thrombolysis].
abstract: OBJECTIVE
  To study the effect of FA-I, a novel fibrinolytic enzyme isolated from the metabolite of Arthrobacter sp. DR-536, on thrombosis and thrombolysis in vivo.
  
  
  METHODS
  The anticoagulated blood model of mise were administered with FA-I orally. The venous thrombogenesis inhibition model of rats were administered with FA-I by intestinal route. The carotid thrombosis model of rabbits were given FA-I intravenously. The clotting time (CT), prothrombin time (PT), activated partial thromboplastin time (APTT), thrombin time (TT), Euglobulin lysis time (ELT), fibrinogen (FIG) and hemorheology condition were analyzed.
  
  
  RESULTS
  FA-I prolonged the CT, PT, APTT and TT, and decreased ELT and FIG significantly (P < 0.05 or P < 0.01). FA-I also improved the hemorheology conditions in the rabbits.
  
  
  CONCLUSIONS
  Both intravenous injection and oral administration of FA-I are effective in thrombosis and thrombolysis. FA-I could become a pragmatic venoclysis thrombolytic drug or a peroral thrombolytic drug.
authors.affiliation: College of Animal Science and Technology, Northwest A&F University, Yangling 712100, China.
authors.forename: Gao-xue
authors.initials: GX
authors.lastname: Wang
authors.forename: Chao-jun
authors.initials: CJ
authors.lastname: Liang
authors.forename: Jia-yun
authors.initials: JY
authors.lastname: Yao
authors.forename: Ju-lin
authors.initials: JL
authors.lastname: Yuan
authors.forename: Jian-fu
authors.initials: JF
authors.lastname: Wang
mesh_terms: D000818:Animals; D001419:Bacteria / Q000201:enzymology*; D001426:Bacterial Proteins / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D002341:Carotid Artery Thrombosis / Q000188:drug therapy*; D004795:Enzyme Stability; D005285:Fermentation; D005341:Fibrinolysin / Q000737:chemistry; D005342:Fibrinolysis; D005343:Fibrinolytic Agents / Q000302:isolation & purification* / Q000627:therapeutic use; D011817:Rabbits; D051381:Rats; D020246:Venous Thrombosis / Q000188:drug therapy*
chemical_list: D001426:Bacterial Proteins; D005343:Fibrinolytic Agents; D005341:Fibrinolysin