title: Genetic analysis of an F(2) intercross between two chicken lines divergently selected for body-weight.
abstract: BACKGROUND
  We have performed Quantitative Trait Loci (QTL) analysis of an F(2) intercross between two chicken lines divergently selected for juvenile body-weight. In a previous study 13 identified loci with effects on body-weight, only explained a small proportion of the large variation in the F(2) population. Epistatic interaction analysis however, indicated that a network of interacting loci with large effect contributed to the difference in body-weight of the parental lines. This previous analysis was, however, based on a sparse microsatellite linkage map and the limited coverage could have affected the main conclusions. Here we present a revised QTL analysis based on a high-density linkage map that provided a more complete coverage of the chicken genome. Furthermore, we utilized genotype data from ~13,000 SNPs to search the genome for potential selective sweeps that have occurred in the selected lines.
  
  
  RESULTS
  We constructed a linkage map comprising 434 genetic markers, covering 31 chromosomes but leaving seven microchromosomes uncovered. The analysis showed that seven regions harbor QTL that influence growth. The pair-wise interaction analysis identified 15 unique QTL pairs and notable is that nine of those involved interactions with a locus on chromosome 7, forming a network of interacting loci. The analysis of ~13,000 SNPs showed that a substantial proportion of the genetic variation present in the founder population has been lost in either of the two selected lines since ~60% of the SNPs polymorphic among lines showed fixation in one of the lines. With the current marker coverage and QTL map resolution we did not observe clear signs of selective sweeps within QTL intervals.
  
  
  CONCLUSIONS
  The results from the QTL analysis using the new improved linkage map are to a large extent in concordance with our previous analysis of this pedigree. The difference in body-weight between the parental chicken lines is caused by many QTL each with a small individual effect. Although the increased chromosomal marker coverage did not lead to the identification of additional QTL, we were able to refine the localization of QTL. The importance of epistatic interaction as a mechanism contributing significantly to the remarkable selection response was further strengthened because additional pairs of interacting loci were detected with the improved map.
authors.affiliation: Department of Medical Biochemistry and Microbiology, Uppsala University, BMC, Uppsala, Sweden. per.wahlberg@imbim.uu.se
authors.forename: Per
authors.initials: P
authors.lastname: Wahlberg
authors.forename: Orjan
authors.initials: O
authors.lastname: Carlborg
authors.forename: Mario
authors.initials: M
authors.lastname: Foglio
authors.forename: Xavier
authors.initials: X
authors.lastname: Tordoir
authors.forename: Ann-Christine
authors.initials: AC
authors.lastname: Syvänen
authors.forename: Mark
authors.initials: M
authors.lastname: Lathrop
authors.forename: Ivo G
authors.initials: IG
authors.lastname: Gut
authors.forename: Paul B
authors.initials: PB
authors.lastname: Siegel
authors.forename: Leif
authors.initials: L
authors.lastname: Andersson
mesh_terms: D000818:Animals; D001835:Body Weight / Q000235:genetics*; D002645:Chickens / Q000235:genetics* / Q000254:growth & development; D002874:Chromosome Mapping; D003433:Crosses, Genetic; D004843:Epistasis, Genetic; D005260:Female; D005787:Gene Frequency; D005819:Genetic Markers; D005838:Genotype; D008297:Male; D018895:Microsatellite Repeats; D010641:Phenotype; D020641:Polymorphism, Single Nucleotide; D040641:Quantitative Trait Loci
chemical_list: D005819:Genetic Markers