Rapid and high-throughput analysis of N-glycans from ovarian cancer serum using a 96-well plate platform.
We present a rapid and high-throughput human serum N-glycan preparation technology using 96-well plate-based procedures. The released N-glycans from polyvinylidene fluoride (PVDF) membrane filter plate are subsequently loaded to porous graphitic carbon (PGC) containing a 96-well plate to remove salts and other contaminants without sacrificing accuracy or reproducibility. This robust glycan preparation technology is applied to ovarian cancer diagnosis using 5 microl of patient serum.
Kim
Yun-Gon
YG
Institute of Molecular Biology and Genetics, Seoul National University, Seoul 151-742, Republic of Korea.
Jeong
Hee-Jin
HJ
Jang
Kyoung-Soon
KS
Yang
Yung-Hun
YH
Song
Yong-Sang
YS
Chung
Junho
J
Kim
Byung-Gee
BG
D005260:Female; D006801:Humans; D008567:Membranes, Artificial; D010051:Ovarian Neoplasms / Q000097:blood / Q000737:chemistry*; D011134:Polysaccharides / Q000097:blood* / Q000302:isolation & purification; D011145:Polyvinyls / Q000737:chemistry; D052616:Solid Phase Extraction / Q000295:instrumentation* / Q000379:methods; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D005465:Fluorocarbon Polymers
D008567:Membranes, Artificial; D011134:Polysaccharides; D011145:Polyvinyls; C024865:polyvinylidene fluoride; D005465:Fluorocarbon Polymers