title: Metabolic depression during aestivation does not involve remodelling of membrane fatty acids in two Australian frogs.
abstract: Changes in membrane lipid composition (membrane remodelling) have been associated with metabolic depression in some aestivating snails but has not been studied in aestivating frogs. This study examined the membrane phospholipid composition of two Australian aestivating frog species Cyclorana alboguttata and Cyclorana australis. The results showed no major membrane remodelling of tissue in either frog species, or in mitochondria of C. alboguttata due to aestivation. Mitochondrial membrane remodelling was not investigated in C. australis. Where investigated in C. alboguttata, total protein and phospholipid content, and citrate synthase (CS) and cytochrome c oxidase (CCO) activities in tissues and mitochondria mostly did not change with aestivation in liver. In skeletal muscle, however, CS and CCO activities, mitochondrial and tissue phospholipids, and mitochondrial protein decreased with aestivation. These decreases in muscle indicate that skeletal muscle mitochondrial content may decrease during aestivation. Na(+)K(+)ATPase activity of both frog species showed no effect of aestivation. In C. alboguttata different fat diets had a major effect on both tissue and mitochondrial phospholipid composition indicating an ability to remodel membrane composition that is not utilised in aestivation. Therefore, changes in lipid composition associated with some aestivating snails do not occur during aestivation in these Australian frogs.
authors.affiliation: Department of Biology, University of the South, Sewanee, TN, 37383-1000, USA. nberner@sewanee.edu
authors.forename: Nancy J
authors.initials: NJ
authors.lastname: Berner
authors.forename: P L
authors.initials: PL
authors.lastname: Else
authors.forename: A J
authors.initials: AJ
authors.lastname: Hulbert
authors.forename: B L
authors.initials: BL
authors.lastname: Mantle
authors.forename: R L
authors.initials: RL
authors.lastname: Cramp
authors.forename: C E
authors.initials: CE
authors.lastname: Franklin
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D001001:Anura / Q000502:physiology*; D049628:Body Size; D001835:Body Weight; D003058:Cockroaches / Q000737:chemistry; D004041:Dietary Fats / Q000008:administration & dosage; D004734:Energy Metabolism / Q000502:physiology*; D004956:Estivation / Q000502:physiology*; D005227:Fatty Acids / Q000032:analysis*; D005229:Fatty Acids, Monounsaturated / Q000008:administration & dosage / Q000032:analysis; D043371:Fatty Acids, Omega-6 / Q000008:administration & dosage / Q000032:analysis; D006135:Gryllidae / Q000737:chemistry; D007668:Kidney / Q000737:chemistry / Q000201:enzymology; D008099:Liver / Q000737:chemistry / Q000201:enzymology / Q000254:growth & development; D008930:Mitochondria, Liver / Q000737:chemistry / Q000201:enzymology; D008931:Mitochondria, Muscle / Q000737:chemistry / Q000201:enzymology; D018482:Muscle, Skeletal / Q000737:chemistry / Q000201:enzymology; D015876:Northern Territory; D009929:Organ Size; D010743:Phospholipids / Q000737:chemistry*; D011793:Queensland; D011897:Random Allocation; D012621:Seasons
chemical_list: D004041:Dietary Fats; D005227:Fatty Acids; D005229:Fatty Acids, Monounsaturated; D043371:Fatty Acids, Omega-6; D010743:Phospholipids