Factors effecting expression of vaccines in microalgae.
PhycoBiologics is developing an oral vaccine delivery system using vaccines expressed in the chloroplast of microalgae. Despite many advances in plastid transformation technology, levels of expression remain inconsistent. We have concluded that the main factors affecting the level of recombinant protein expression in the chloroplast of Chlamydomonas are: codon optimization, protease activity, protein toxicity and transformation-associated genotypic modification.
Surzycki
Raymond
R
PhycoBiologics Inc, Indianapolis IN, Bloomington, IN 47401, USA.
Greenham
Katie
K
Kitayama
Kaoru
K
Dibal
Flora
F
Wagner
Richard
R
Rochaix
Jean-David
JD
Ajam
Tarek
T
Surzycki
Stefan
S
D000818:Animals; D030801:Animals, Genetically Modified; D015153:Blotting, Western; D002696:Chlamydomonas / Q000235:genetics*; D003062:Codon; D005838:Genotype; D014614:Vaccines, Synthetic / Q000235:genetics*
D003062:Codon; D014614:Vaccines, Synthetic