Taking fingerprints of DNA polymerases: multiplex enzyme profiling on DNA arrays.
A novel screening approach based on an oligonucleotide-addressing enzyme assay enables multiplexed simultaneous profiling of DNA polymerases in nanoliter volumes in terms of their different properties. This approach was used to identify enzymes with altered properties out of a library of protein mutants.
Kranaster
Ramon
R
Department of Chemistry and Konstanz Research School Chemical Biology, University of Konstanz, Universitätsstrasse 10, 78457 Konstanz, Germany.
Marx
Andreas
A
D020137:Base Pair Mismatch; D001483:Base Sequence; D016172:DNA Fingerprinting; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism*; D005456:Fluorescent Dyes / Q000737:chemistry; D050505:Mutant Proteins / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D011806:Quinolinium Compounds / Q000737:chemistry; D019809:Streptavidin / Q000737:chemistry
C481052:Alexa fluor 546; D005456:Fluorescent Dyes; D050505:Mutant Proteins; D011806:Quinolinium Compounds; D019809:Streptavidin; D004259:DNA-Directed DNA Polymerase