title: In vitro antibacterial activity of porous TiO2-Ag composite layers against methicillin-resistant Staphylococcus aureus.
abstract: The aim of this study was the synthesis of a porous TiO(2)-Ag composite coating and assessment of its in vitro bactericidal activity against methicillin-resistant Staphylococcus aureus. The coating was produced by plasma electrolytic oxidation of Ti-6Al-7Nb medical alloy in a calcium acetate/calcium glycerophosphate electrolyte bearing Ag nanoparticles. Following oxidation, the surface of the titanium substrate was converted into the corresponding oxide (TiO(2)) bearing Ca and P species from the electrolyte. In addition, Ag was detected associated with particles present in the oxide layers. The coatings revealed a porous interconnected structure with pores up to 3 microm in size, a threefold increase in roughness and improved wettability relative to the non-oxidized specimens. The composite TiO(2)-Ag coating showed complete killing of methicillin-resistant S. aureus within 24h in all culture conditions, whereas a 1000-fold increase in bacterial numbers was recorded with the ground titanium specimens and the samples oxidized in the absence of Ag nanoparticles.
authors.affiliation: Delft University of Technology, Department of Materials Science and Engineering, Group of Biomaterials Technology, Mekelweg 2, 2628 CD Delft, The Netherlands. B.S.Necula@tudelft.nl
authors.forename: Bogdan S
authors.initials: BS
authors.lastname: Necula
authors.forename: Lidy E
authors.initials: LE
authors.lastname: Fratila-Apachitei
authors.forename: Sebastian A J
authors.initials: SA
authors.lastname: Zaat
authors.forename: Iulian
authors.initials: I
authors.lastname: Apachitei
authors.forename: Jurek
authors.initials: J
authors.lastname: Duszczyk
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D006801:Humans; D008422:Materials Testing; D055624:Methicillin-Resistant Staphylococcus aureus / Q000187:drug effects*; D008826:Microbial Sensitivity Tests; D008855:Microscopy, Electron, Scanning; D010084:Oxidation-Reduction; D012834:Silver / Q000737:chemistry / Q000494:pharmacology; D013499:Surface Properties; D014025:Titanium / Q000737:chemistry / Q000494:pharmacology; D017155:Wettability
chemical_list: D000900:Anti-Bacterial Agents; D020099:Coated Materials, Biocompatible; C070282:Ti-6Al-7Nb alloy; C009495:titanium dioxide; D012834:Silver; D014025:Titanium