Contributions to the chemistry of human hair: III. Protein chemical aspects of permanent waving treatments.
Synopsis The influence of permanent waving on hair proteins was studied in order to obtain additional information about the chemistry of this cosmetic treatment. It was shown by amino acid analysis that with increasing reduction time during treatment fewer disulphide bonds were reformed in hair during subsequent reoxidation. Simultaneously, an increasing amount of sulphur-containing material is liberated from the hair, as demonstrated by the sulphur balance calculated from the sulphur-containing amino acids. The amount liberated is increased when an extensive soaking of the samples in water between the reduction and reoxidation step is performed. Comparing treatments with the use of reducing solutions of pH values between 7.5 and 10.0, it was found that the largest amount of cystine cleavage occurs at pH 9.0. All hair samples reduced at pH values above pH 8.5 showed incomplete reformation of the disulphide bonds during subsequent reoxidation. This was indicated by the content of free SH-groups and cysteic acid, as quantified by amino acid analysis. The damage to the hair proteins due to permanent waving was further confirmed by the determination of the pronase solubility. The reductive treatment of hair at pH 7.5 led to a relatively low degree of reduction, however all sulphur bonds were reformed during subsequent reoxidation.
Hilterhaus-Bong
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Deutsches Wollforschungsinstitut an der RWTH Aachen, Veltmanplatz 8, 5100 Aachen, FRG.
Zahn
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