Time lapse monitoring of CaCo-2 cell shapes and shape dependence of the distribution of integrin β1 and F-actin on their basal membrane.
CaCo-2 cell line is a model system for cell differentiation. For the effective use of CaCo-2 cells, it is important to understand how their growth depends on environmental conditions. The authors grew them on laminin-1, fibronectin, and collagen-1 adsorbed to glass and polystyrene. The time lapse technique was applied to follow their growth and shape changes for 21.5 h post seeding. The results upgraded the auhtors' previous findings about the series of consecutive shape changes that occur post seeding. Most cells were initially rounded and then they changed shape in two directions. A smaller fraction of cells, which attained cumulus shapes, eventually detached and drifted away. Other cells attained a semispread, transient shape, which was followed by a fully spread shape that was dominant on all protein-coated surfaces. The average time over which cells changed their shape type was different on different surfaces. It was longer on protein-coated glass surfaces than on protein-coated polystyrene surfaces. On collagen-1-coated surfaces, cells spread in the shortest time. Different cell shape types exhibited different spatial distributions of integrin β1, F-actin, and focal adhesions.
Jokhadar
Spela Zemljič
SZ
Institute of Biophysics, Faculty of Medicine, University of Ljubljana, Ljubljana, Slovenia.
Suštar
Vid
V
Svetina
Saša
S
Batista
Urška
U
D000199:Actins / Q000378:metabolism*; D001485:Basement Membrane / Q000378:metabolism*; D018938:Caco-2 Cells; D048430:Cell Shape; D024042:Collagen Type I / Q000378:metabolism; D003470:Culture Media; D004847:Epithelial Cells / Q000166:cytology / Q000378:metabolism; D005353:Fibronectins / Q000378:metabolism; D005898:Glass; D006801:Humans; D019012:Integrin beta1 / Q000378:metabolism*; D007797:Laminin / Q000378:metabolism; D008853:Microscopy; D011137:Polystyrenes; D059008:Time-Lapse Imaging
D000199:Actins; D024042:Collagen Type I; D003470:Culture Media; D005353:Fibronectins; D019012:Integrin beta1; D007797:Laminin; D011137:Polystyrenes; C121346:laminin 1