A proteomics analysis of the effects of chronic hemiparetic stroke on troponin T expression in human vastus lateralis.
Stroke disability is attributed to upper motor neuron deficits resulting from ischemic brain injury. We have developed proteome maps of the Vastus lateralis to examine the effects of ischemic brain injury on paretic skeletal muscle myofilament proteins. Proteomics analyses from seven hemiparetic stroke patients have detected a decrease of three troponin T isoforms in the paretic muscle suggesting that myosin-actin interactions may be attenuated. We propose that ischemic brain injury may prevent troponin T participation in complex formation thereby affecting the protein interactions associated with excitation-contraction coupling. We have also detected a novel skeletal troponin T isoform that has a C-terminal variation. Our data suggest that the decreased slow troponin T isoform pools in the paretic limb may contribute to the gait deficit after stroke. The complexity of the neurological deficit on Vastus lateralis is suggested by the multiple changes in proteins detected by our proteomics mapping.
Rabek
Jeffrey P
JP
Department of Biochemistry and Molecular Biology, The University of Texas Medical Branch, Galveston, TX 77555-0643, USA.
Hafer-Macko
Charlene E
CE
Amaning
James K
JK
Deford
James H
JH
Dimayuga
Vincent L
VL
Madsen
Mark A
MA
Macko
Richard F
RF
Papaconstantinou
John
J
D000368:Aged; D015153:Blotting, Western; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D018995:Myosin Heavy Chains / Q000032:analysis; D010291:Paresis / Q000378:metabolism*; D020543:Proteome; D052097:Quadriceps Muscle / Q000737:chemistry*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D020521:Stroke / Q000378:metabolism*; D020107:Troponin T / Q000032:analysis*
D020543:Proteome; D020107:Troponin T; D018995:Myosin Heavy Chains