Apolipoprotein E limits oxidative stress-induced cell dysfunctions in human adipocytes.
Oxidative stress in adipose tissue constitutes a pathological process involved in obesity-linked metabolic disorders. Apolipoprotein E (apoE), which exhibits antioxidant properties in plasma and brain, is highly produced by adipose tissue and adipocytes. In this study, we investigated the role of apoE in the human adipocyte response to oxidative stress. We first demonstrated that apoE secretion by adipocytes was stimulated by oxidative stress. We also observed that apoE overexpression protected adipocytes from hydrogen peroxide-induced damages, by mitigating intracellular oxidation and exerting extracellular antioxidant properties. Our findings clearly show a novel antioxidant role for apoE in adipose tissue.
Tarnus
Evelyne
E
Laboratoire de Biochimie et Génétique Moléculaire (LBGM), Université de La Réunion, Saint Denis Messag, La Réunion, France.
Wassef
Hanny
H
Carmel
Jean-François
JF
Rondeau
Philippe
P
Roche
Marjolaine
M
Davignon
Jean
J
Bernier
Lise
L
Bourdon
Emmanuel
E
D017667:Adipocytes / Q000187:drug effects / Q000378:metabolism*; D052242:Adiponectin / Q000235:genetics; D000975:Antioxidants / Q000378:metabolism; D001057:Apolipoproteins E / Q000235:genetics / Q000378:metabolism*; D045744:Cell Line, Tumor; D002478:Cells, Cultured; D015870:Gene Expression; D006801:Humans; D006861:Hydrogen Peroxide / Q000633:toxicity; D020738:Leptin / Q000235:genetics; D018384:Oxidative Stress; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D014162:Transfection
C499918:ADIPOQ protein, human; D052242:Adiponectin; D000975:Antioxidants; D001057:Apolipoproteins E; D020738:Leptin; D011994:Recombinant Proteins; D006861:Hydrogen Peroxide