title: Wet chemical synthesis of chitosan hydrogel-hydroxyapatite composite membranes for tissue engineering applications.
abstract: Chitosan, a deacetylated derivative of chitin is a commonly studied biomaterial for tissue-engineering applications due to its biocompatibility, biodegradability, low toxicity, antibacterial activity, wound healing ability and haemostatic properties. However, chitosan has poor mechanical strength due to which its applications in orthopedics are limited. Hydroxyapatite (HAp) is a natural inorganic component of bone and teeth and has mechanical strength and osteoconductive property. In this work, HAp was deposited on the surface of chitosan hydrogel membranes by a wet chemical synthesis method by alternatively soaking the membranes in CaCl(2) (pH 7.4) and Na(2)HPO(4) solutions for different time intervals. These chitosan hydrogel-HAp membranes were characterized using SEM, AFM, EDS, FT-IR and XRD analyses. MTT assay was done to evaluate the biocompatibility of these membranes using MG-63 osteosarcoma cells. The biocompatibility studies suggest that chitosan hydrogel-HAp composite membranes can be useful for tissue-engineering applications.
authors.affiliation: Amrita Centre for Nanosciences, Amrita Institute of Medical Sciences and Research Centre, Amrita Viswa Vidyapeetham University, Kochi, India.
authors.forename: K
authors.initials: K
authors.lastname: Madhumathi
authors.forename: K T
authors.initials: KT
authors.lastname: Shalumon
authors.forename: V V Divya
authors.initials: VV
authors.lastname: Rani
authors.forename: H
authors.initials: H
authors.lastname: Tamura
authors.forename: T
authors.initials: T
authors.lastname: Furuike
authors.forename: N
authors.initials: N
authors.lastname: Selvamurugan
authors.forename: S V
authors.initials: SV
authors.lastname: Nair
authors.forename: R
authors.initials: R
authors.lastname: Jayakumar
mesh_terms: D048271:Chitosan / Q000737:chemistry*; D017886:Durapatite / Q000737:chemistry*; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000138:chemical synthesis* / Q000737:chemistry*; D008422:Materials Testing / Q000379:methods; D008567:Membranes, Artificial; D018625:Microscopy, Atomic Force; D008855:Microscopy, Electron, Scanning; D017550:Spectroscopy, Fourier Transform Infrared; D023822:Tissue Engineering / Q000295:instrumentation* / Q000379:methods; D014961:X-Ray Diffraction
chemical_list: D008567:Membranes, Artificial; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D048271:Chitosan; D017886:Durapatite