Induction of lipocalin-type prostaglandin D synthase in mouse heart under hypoxemia.
Hypoxemia is a common manifestation of various disorders and generates pressure overload to the heart. Here we analyzed the expression of lipocalin-type prostaglandin D synthase (L-PGDS) in the heart of C57BL/6 mice kept under normobaric hypoxia (10% O2) that generates hemodynamic stress. Northern and Western blot analyses revealed that the expression levels of L-PGDS mRNA and protein were significantly increased (> twofold) after 14 days of hypoxia, compared to the mice kept under normoxia. Immunohistochemical analysis indicated that L-PGDS was increased in the myocardium of auricles and ventricles and the pulmonary venous myocardium at 28 days of hypoxia. Moreover, using C57BL/6 mice lacking heme oxygenase-2 (HO-2(-/-)), a model of chronic hypoxemia, we showed that the expression level of L-PGDS protein was twofold higher in the heart than that of wild-type mouse. L-PGDS expression is induced in the myocardium under hypoxemia, which may reflect the adaptation to the hemodynamic stress.
Han
Feng
F
Department of Molecular Biology and Applied Physiology, Tohoku University School of Medicine, Sendai 980-8575, Japan.
Takeda
Kazuhisa
K
Ishikawa
Kazunobu
K
Ono
Masao
M
Date
Fumiko
F
Yokoyama
Satoru
S
Furuyama
Kazumichi
K
Shinozawa
Yotaro
Y
Urade
Yoshihiro
Y
Shibahara
Shigeki
S
D000818:Animals; D006419:Heme Oxygenase (Decyclizing) / Q000235:genetics; D000860:Hypoxia / Q000201:enzymology*; D019746:Intramolecular Oxidoreductases / Q000096:biosynthesis*; D054834:Lipocalins / Q000096:biosynthesis*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008817:Mice, Mutant Strains; D009206:Myocardium / Q000201:enzymology*
D054834:Lipocalins; D006419:Heme Oxygenase (Decyclizing); C105245:heme oxygenase-2; D019746:Intramolecular Oxidoreductases; C022466:prostaglandin R2 D-isomerase