title: Paleogenomics in a temperate environment: shotgun sequencing from an extinct Mediterranean caprine.
abstract: BACKGROUND
  Numerous endemic mammals, including dwarf elephants, goats, hippos and deers, evolved in isolation in the Mediterranean islands during the Pliocene and Pleistocene. Most of them subsequently became extinct during the Holocene. Recently developed high-throughput sequencing technologies could provide a unique tool for retrieving genomic data from these extinct species, making it possible to study their evolutionary history and the genetic bases underlying their particular, sometimes unique, adaptations. METHODOLOGY/PRINCIPALS FINDINGS: A DNA extraction of a approximately 6,000 year-old bone sample from an extinct caprine (Myotragus balearicus) from the Balearic Islands in the Western Mediterranean, has been subjected to shotgun sequencing with the GS FLX 454 platform. Only 0.27% of the resulting sequences, identified from alignments with the cow genome and comprising 15,832 nucleotides, with an average length of 60 nucleotides, proved to be endogenous.
  
  
  CONCLUSIONS
  A phylogenetic tree generated with Myotragus sequences and those from other artiodactyls displays an identical topology to that generated from mitochondrial DNA data. Despite being in an unfavourable thermal environment, which explains the low yield of endogenous sequences, our study demonstrates that it is possible to obtain genomic data from extinct species from temperate regions.
authors.affiliation: Institute of Evolutionary Biology (UPF-CSIC), Barcelona, Spain.
authors.forename: Oscar
authors.initials: O
authors.lastname: Ramírez
authors.forename: Elena
authors.initials: E
authors.lastname: Gigli
authors.forename: Pere
authors.initials: P
authors.lastname: Bover
authors.forename: Josep Antoni
authors.initials: JA
authors.lastname: Alcover
authors.forename: Jaume
authors.initials: J
authors.lastname: Bertranpetit
authors.forename: Jose
authors.initials: J
authors.lastname: Castresana
authors.forename: Carles
authors.initials: C
authors.lastname: Lalueza-Fox
mesh_terms: D019943:Amino Acid Substitution; D000818:Animals; D020029:Base Pairing; D002980:Climate; D053476:Extinction, Biological; D016678:Genome / Q000235:genetics; D023281:Genomics; D006041:Goats / Q000235:genetics*; D016013:Likelihood Functions; D008521:Mediterranean Islands; D010163:Paleontology / Q000379:methods*; D010802:Phylogeny; D017422:Sequence Analysis, DNA / Q000379:methods*; D013030:Spain