title: Quantitative real-time PCR assay for QPX (Thraustochytriidae), a parasite of the hard clam (Mercenaria mercenaria).
abstract: We developed a real-time quantitative PCR (qPCR) assay targeting the rRNA internal transcribed spacer region of the hard clam pathogen QPX. The qPCR assay was more sensitive than was histology in detecting clams with light QPX infections. QPX was detected in 4 of 43 sediment samples but in none of 40 seawater samples.
authors.affiliation: School of Marine and Atmospheric Sciences, Stony Brook University, Stony Brook, NY 11794-5000, USA.
authors.forename: Qianqian
authors.initials: Q
authors.lastname: Liu
authors.forename: Bassem
authors.initials: B
authors.lastname: Allam
authors.forename: Jackie L
authors.initials: JL
authors.lastname: Collier
mesh_terms: D000818:Animals; D017931:DNA Primers / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D019015:Geologic Sediments / Q000469:parasitology; D049891:Mercenaria / Q000469:parasitology*; D008969:Molecular Sequence Data; D010271:Parasites / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D017422:Sequence Analysis, DNA; D013997:Time Factors
chemical_list: D017931:DNA Primers; D004275:DNA, Ribosomal