title: TCRalphabeta + CD8+ and TCRgammadelta+ lymphocytes inhibit the capability of peritoneal macrophages to induce contact hypersensitivity.
abstract: Macrophages (Mf) play an important role in induction and regulation of the immune response. It was shown previously that subcutaneous injection of hapten conjugated macrophages (TNP-Mf) induces the contact hypersensitivity (CHS) response, whereas intravenous (i.v.) or intraperitioneal administration of TNP-Mf results in unresponsiveness as a result of induced T suppressor (Ts) cells. The aim of this study was to determine if different T cell populations influence macrophages to become inducers of immunological suppression. Our findings show that indeed i.v. injection of TNP labeled macrophages isolated from control mice into syngenic recipients induces unresponsiveness. However, i.v. administration of TNP substituted macrophages isolated from TCRalpha-/-, TCRdelta-/- and beta2m-/- mice induces strong CHS similar to that observed after skin painting with TNP-C1. Moreover, it was shown that TNP conjugated macrophages isolated from CD1d-/- mice were still able to promote immunosuppression when injected intravenously. This suggests that TCRalphabeta+ CD8+ and TCRgammadelta+ lymphocytes stimulate macrophages to induce immunosuppression instead of a strong CHS reaction, whereas CD1d dependent NKT cells are not involved in negative regulation of macrophage function.
authors.affiliation: Department of Human Developmental Biology, Collegium Medicum, Jagiellonian University, Kopernika 7, 31-034 Kraków, Poland.
authors.forename: Monika
authors.initials: M
authors.lastname: Majewska
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Zajac
authors.forename: Magdalena
authors.initials: M
authors.lastname: Zemelka
authors.forename: Piotr
authors.initials: P
authors.lastname: Sura
authors.forename: Andrzej
authors.initials: A
authors.lastname: Gryglewski
authors.forename: Marian
authors.initials: M
authors.lastname: Szczepanik
mesh_terms: D000818:Animals; D002450:Cell Communication / Q000276:immunology*; D003877:Dermatitis, Contact / Q000276:immunology*; D017737:Macrophages, Peritoneal / Q000276:immunology*; D008297:Male; D051379:Mice; D008808:Mice, Inbred CBA; D018345:Mice, Knockout; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000276:immunology*; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology*
chemical_list: D016693:Receptors, Antigen, T-Cell, alpha-beta; D016692:Receptors, Antigen, T-Cell, gamma-delta