title: Sequential analysis of RNA synthesis by microchip electrophoresis.
abstract: We describe the potential of microchip electrophoresis with a Hitachi SV1100, which can be used to evaluate the integrity of total RNA, for the analysis of synthesized RNA. There was little interference by DNA and/or the components of the in vitro transcription system with the microchip electrophoresis. The fluorescence intensity corresponding to the synthesized RNA increased in a time-dependent manner as to the RNA synthesis reaction on sequential analysis. A result can be obtained in 160 s and only 1/10 aliquots of samples, compared with the conventional method, are required. These results indicate the potential of microchip electrophoresis for sequential analysis of RNA synthesis.
authors.affiliation: Department of Molecular and Pharmaceutical Biotechnology, Graduate School of Pharmaceutical Sciences, University of Tokushima, Shomachi 1-78, Tokushima 770-8505, Japan.
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Umemoto
authors.forename: Masatoshi
authors.initials: M
authors.lastname: Kataoka
authors.forename: Shouki
authors.initials: S
authors.lastname: Yatsushiro
authors.forename: Masahiro
authors.initials: M
authors.lastname: Watanabe
authors.forename: Jun-Ichi
authors.initials: J
authors.lastname: Kido
authors.forename: Rei
authors.initials: R
authors.lastname: Kakuhata
authors.forename: Takenori
authors.initials: T
authors.lastname: Yamamoto
authors.forename: Yasuo
authors.initials: Y
authors.lastname: Shinohara
authors.forename: Yoshinobu
authors.initials: Y
authors.lastname: Baba
mesh_terms: D046209:Electrophoresis, Microchip / Q000295:instrumentation / Q000379:methods*; D005456:Fluorescent Dyes / Q000737:chemistry; D012313:RNA / Q000032:analysis / Q000096:biosynthesis*
chemical_list: D005456:Fluorescent Dyes; D012313:RNA