In vitro release of growth factors from platelet-rich fibrin (PRF): a proposal to optimize the clinical applications of PRF.
OBJECTIVE
Determine the release of growth factors (GF) from platelet-rich fibrin (PRF) and supernatant serum to optimize clinical use.


METHODS
Platelet-derived growth factors-AB (PDGF-AB), transforming growth factor-beta1 (TGF-beta1), vascular endothelial growth factor (VEGF), epidermal growth factor (EGF), and insulin-like growth factor-1 (IGF-1) were quantified in PRF releasate and in the supernatant serum (N = 8) over 300 minutes after clot formation. Protein profiles were determined by SDS-PAGE.


RESULTS
Mean quantity of PDGF-AB, TGF-ss1, VEGF, and EGF in PRF releasate increased significantly to about 52, 72, 1, and 3 ng, respectively, whereas mean IGF-1 content remained at 250 ng. GF was also found in serum supernatant. Protein profiles of the releasates and the supernatant serum were similar.


CONCLUSIONS
The PRF membrane should be used immediately after formation to maximize release of GF to the surgical site. The remaining fluid can be recovered as an additional source of GF for grafting.
Su
Chen Yao
CY
Department of Dentistry, National Yang-Ming University, Taipei, Taiwan. henrycysu@gmail.com
Kuo
Ya Po
YP
Tseng
Yu Hong
YH
Su
Ching-Hua
CH
Burnouf
Thierry
T
D001777:Blood Coagulation / Q000502:physiology; D001792:Blood Platelets / Q000502:physiology*; D004591:Electrophoresis, Polyacrylamide Gel; D004815:Epidermal Growth Factor / Q000032:analysis / Q000378:metabolism; D004906:Erythrocyte Count; D005337:Fibrin / Q000502:physiology*; D006801:Humans; D007334:Insulin-Like Growth Factor I / Q000032:analysis / Q000378:metabolism; D036341:Intercellular Signaling Peptides and Proteins / Q000032:analysis / Q000378:metabolism*; D007958:Leukocyte Count; D010976:Platelet Count; D010982:Platelet-Derived Growth Factor / Q000032:analysis / Q000378:metabolism; D044967:Serum / Q000502:physiology; D053773:Transforming Growth Factor beta1 / Q000032:analysis / Q000378:metabolism; D042461:Vascular Endothelial Growth Factor A / Q000032:analysis / Q000378:metabolism
D036341:Intercellular Signaling Peptides and Proteins; D010982:Platelet-Derived Growth Factor; D053773:Transforming Growth Factor beta1; D042461:Vascular Endothelial Growth Factor A; C095477:platelet-derived growth factor AB; D004815:Epidermal Growth Factor; D007334:Insulin-Like Growth Factor I; D005337:Fibrin