title: Differences in the antibody response and survival of genetically different varieties of common carp (Cyprinus carpio L.) vaccinated with a commercial Aeromonas salmonicida/A. hydrophila vaccine and challenged with A. hydrophila.
abstract: Males of two strains of carp, wild Duna (D), and inbred Szarvas 22 (22), were selected for high and low stress response. Two purebreds of D and 22, from randomly chosen parents and four crosses, 22 x 22-L (low stress response), 22 x 22-H (high stress response), 22 x D-L (low stress response) and 22 x D-H (high stress response) from selected stress response parents were produced and vaccinated with a commercial Aeromonas salmonicida/Aeromonas hydrophila vaccine and their circulating antibody response evaluated 1, 3, 5, and 7 weeks post-vaccination by ELISA. Significantly higher titres of circulatory antibodies against A. hydrophila were found in the families 22 and cross 22 x 22-L compared to other groups. The development of circulatory antibodies against A. hydrophila in all crosses having at least one D parent was low and remained low throughout the experiment. The level of circulatory antibodies against atypical A. salmonicida in the inbred strain increased following a booster vaccination with the highest values measured in inbred strain 22 and cross 22 x 22 L. The different varieties of carp had different levels of survival against experimental challenge with A. hydrophila. The greatest survival was obtained in strain 22 and cross 22 x 22-L, while ~90% of D wild carp and cross 22 x D (independent of their stress response) died. Survival results correlated well with the antibody response of the different groups: 22 and 22 x 22-L had the highest antibody titres against A. hydrophila and the greatest level of survival.
authors.affiliation: Research Institute for Fisheries, Aquaculture and Irrigation (HAKI), 5541 Szarvas, Hungary.
authors.forename: Zsigmond
authors.initials: Z
authors.lastname: Jeney
authors.forename: Tímea
authors.initials: T
authors.lastname: Rácz
authors.forename: Kim D
authors.initials: KD
authors.lastname: Thompson
authors.forename: Saravanane
authors.initials: S
authors.lastname: Poobalane
authors.forename: László
authors.initials: L
authors.lastname: Ardó
authors.forename: Alexandra
authors.initials: A
authors.lastname: Adams
authors.forename: Galina
authors.initials: G
authors.lastname: Jeney
mesh_terms: D016980:Aeromonas hydrophila / Q000276:immunology*; D048409:Aeromonas salmonicida / Q000276:immunology*; D000704:Analysis of Variance; D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood*; D001428:Bacterial Vaccines / Q000627:therapeutic use*; D002347:Carps; D003433:Crosses, Genetic; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005393:Fish Diseases / Q000382:microbiology* / Q000517:prevention & control*; D016905:Gram-Negative Bacterial Infections / Q000276:immunology / Q000517:prevention & control / Q000662:veterinary*; D008297:Male; D013045:Species Specificity; D016019:Survival Analysis
chemical_list: D000907:Antibodies, Bacterial; D001428:Bacterial Vaccines