[Effects of nonylphenol on proliferation and anti-oxidative functions of Carassius auratus primary culture hepatecytes].
The study on the proliferation and anti-oxidative functions of Carassius auratus primary culture hepatecytes under effects of different concentration nonylphenol showed that at all test concentrations of nonylphenol, the proliferation of the hepatecytes was inhibited. High concentration (10(-3) mol x L(-1)) nonylphenol had significant inhibitory effect, and induced an apparent morphological alteration of the hepatecytes. Nonylphenol upset the balance of hepatecytes anti-oxidative system through decreasing the activities of superoxide dismutase (SOD) and catalase (CAT) while increasing the hydroxyl free radical concentration. Nonylphenol also caused the oxidative damage on the hepatecytes, resulting in the increase of malondialdehyde (MDA) concentration in cell culture medium. The nonylphenol-induced oxidative stress engendered a series of in vitro toxic effects on the primary culture hepatecytes of C. auratus.
Zhang
Yi
Y
College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China. yizhang@stmail.shou.edu.cn
Zhang
Gao-Feng
GF
Wei
Hua
H
D000818:Animals; D002374:Catalase / Q000378:metabolism; D049109:Cell Proliferation; D002478:Cells, Cultured; D052244:Endocrine Disruptors / Q000633:toxicity; D006054:Goldfish / Q000378:metabolism*; D022781:Hepatocytes / Q000166:cytology*; D008099:Liver / Q000166:cytology / Q000378:metabolism*; D018384:Oxidative Stress / Q000502:physiology*; D010636:Phenols / Q000633:toxicity*; D013482:Superoxide Dismutase / Q000378:metabolism
D052244:Endocrine Disruptors; D010636:Phenols; C025256:nonylphenol; D002374:Catalase; D013482:Superoxide Dismutase