title: Aberrant expression of serum amyloid A in head and neck squamous cell carcinoma.
abstract: Serum amyloid A (SAA) is an acute-phase reactant, the blood level of which is often elevated in response to some types of neoplasia. Here, we investigated expression of the gene SAA1 and the protein SAA in head and neck squamous cell carcinoma (HNSCC) and normal oral mucosal tissues as well as blood SAA levels in HNSCC patients. Also, we investigated the effects of inhibiting signal transducer and activator of transcription 3 (STAT3) signaling on SAA1 expression in the HNSCC cell line SAS. Serum SAA levels in HNSCC patients were significantly higher than those in healthy volunteers. In addition, real-time quantitative reverse transcription-polymerase chain reaction analysis revealed a significantly higher SAA1 expression level in HNSCC than in normal mucosa (P < 0.0001). Furthermore, Western blot and immunohistochemical analyzes revealed that high expression of SAA in carcinomas was detected predominantly in tumor cells, but not in normal mucosal tissues. An inhibitor of STAT3 activation, AG490, significantly reduced SAA1 expression in SAS cells. These data demonstrated that SAA was up-regulated in HNSCC through the Janus kinase-STAT3 pathway.
authors.affiliation: Department of Oral and Maxillofacial Surgery, Graduate School of Medical Sciences, Kumamoto University, Kumamoto.
authors.forename: Satoru
authors.initials: S
authors.lastname: Shinriki
authors.forename: Mitsuharu
authors.initials: M
authors.lastname: Ueda
authors.forename: Kazutoshi
authors.initials: K
authors.lastname: Ota
authors.forename: Masaaki
authors.initials: M
authors.lastname: Nakamura
authors.forename: Mareina
authors.initials: M
authors.lastname: Kudo
authors.forename: Mutsuko
authors.initials: M
authors.lastname: Ibusuki
authors.forename: Jaemi
authors.initials: J
authors.lastname: Kim
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Yoshitake
authors.forename: Daiki
authors.initials: D
authors.lastname: Fukuma
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Jono
authors.forename: Jun-Ichi
authors.initials: J
authors.lastname: Kuratsu
authors.forename: Masanori
authors.initials: M
authors.lastname: Shinohara
authors.forename: Yukio
authors.initials: Y
authors.lastname: Ando
mesh_terms: D000368:Aged; D015153:Blotting, Western; D002294:Carcinoma, Squamous Cell / Q000097:blood / Q000473:pathology*; D045744:Cell Line, Tumor; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005260:Female; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics; D005881:Gingiva / Q000473:pathology; D006258:Head and Neck Neoplasms / Q000097:blood / Q000473:pathology*; D006801:Humans; D007150:Immunohistochemistry; D053612:Janus Kinases / Q000187:drug effects; D008297:Male; D009061:Mouth Mucosa / Q000473:pathology; D009367:Neoplasm Staging; D020133:Reverse Transcriptase Polymerase Chain Reaction; D050796:STAT3 Transcription Factor / Q000032:analysis / Q000037:antagonists & inhibitors / Q000235:genetics; D000685:Serum Amyloid A Protein / Q000032:analysis* / Q000037:antagonists & inhibitors / Q000235:genetics; D020032:Tyrphostins / Q000494:pharmacology; D015854:Up-Regulation / Q000187:drug effects
chemical_list: D004791:Enzyme Inhibitors; C521404:SAA1 protein, human; D050796:STAT3 Transcription Factor; C494086:STAT3 protein, human; D000685:Serum Amyloid A Protein; D020032:Tyrphostins; C095512:alpha-cyano-(3,4-dihydroxy)-N-benzylcinnamide; D053612:Janus Kinases