A process for the production of ectoine and poly(3-hydroxybutyrate) by Halomonas boliviensis.
The paper reports a study involving the use of Halomonas boliviensis, a moderate halophile, for co-production of compatible solute ectoine and biopolyester poly(3-hydroxybutyrate) (PHB) in a process comprising two fed-batch cultures. Initial investigations on the growth of the organism in a medium with varying NaCl concentrations showed the highest level of intracellular accumulation of ectoine (0.74 g L(-1)) at 10-15% (w/v) NaCl, while at 15% (w/v) NaCl, the presence of hydroxyectoine (50 mg L(-1)) was also noted. On the other hand, the maximum cell dry weight and PHB concentration of 10 and 5.8 g L(-1), respectively, were obtained at 5-7.5% (w/v) NaCl. A process comprising two fed-batch cultivations was developed-the first culture aimed at obtaining high cell mass and the second for achieving high yields of ectoine and PHB. In the first fed-batch culture, H. boliviensis was grown in a medium with 4.5% (w/v) NaCl and sufficient levels of monosodium glutamate, NH (4) (+) , and PO (4) (3-) . In the second fed-batch culture, the NaCl concentration was increased to 7.5% (w/v) to trigger ectoine synthesis, while nitrogen and phosphorus sources were fed only during the first 3 h and then stopped to favor PHB accumulation. The process resulted in PHB yield of 68.5 wt.% of cell dry weight and volumetric productivity of about 1 g L(-1) h(-1) and ectoine concentration, content, and volumetric productivity of 4.3 g L(-1), 7.2 wt.%, and 2.8 g L(-1) day(-1), respectively. At salt concentration of 12.5% (w/v) during the second cultivation, the ectoine content was increased to 17 wt.% and productivity to 3.4 g L(-1) day(-1).
Guzmán
Héctor
H
Department of Biotechnology, Lund University, Sweden.
Van-Thuoc
Doan
D
Martín
Javier
J
Hatti-Kaul
Rajni
R
Quillaguamán
Jorge
J
D000599:Amino Acids, Diamino / Q000096:biosynthesis*; D019149:Bioreactors; D003470:Culture Media / Q000737:chemistry; D020632:Halomonas / Q000254:growth & development / Q000378:metabolism*; D006885:Hydroxybutyrates / Q000378:metabolism*; D007218:Industrial Microbiology / Q000379:methods; D011091:Polyesters / Q000378:metabolism*; D012965:Sodium Chloride / Q000737:chemistry; D000094122:Polyhydroxybutyrates
D000599:Amino Acids, Diamino; D003470:Culture Media; D006885:Hydroxybutyrates; D011091:Polyesters; D012965:Sodium Chloride; C003182:poly-beta-hydroxybutyrate; D000094122:Polyhydroxybutyrates; C045628:ectoine