A system for the determination of planar lipid bilayer breakdown voltage and its applications.
In this paper, we focus on measurement principles used in electroporation studies on planar lipid bilayers. In particular, we point out the voltage-clamp measurement principle that has great importance when the breakdown voltage of a planar lipid bilayer is under consideration; however, it is also appropriate for the determination of other planar lipid bilayer electrical properties such as resistance and capacitance. A new experimental system that is based on the voltage-clamp measurement principle is described. With the use of a generator that can generate arbitrary-type signals, many specific shapes of a voltage signal could be generated, and therefore, the experimental system is appropriate for a broad spectrum of measurements.
Kramar
Peter
P
Faculty of Electrical Engineering, University of Ljubljana, Ljubljana SI-1000, Slovenia. peter.kramar@fe.uni-lj.si
Miklavcic
Damijan
D
Lebar
Alenka Macek
AM
D004558:Electric Stimulation / Q000295:instrumentation*; D004563:Electrochemistry / Q000379:methods*; D004574:Electromagnetic Fields; D018274:Electroporation / Q000295:instrumentation*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D008051:Lipid Bilayers / Q000737:chemistry*; D018408:Patch-Clamp Techniques / Q000295:instrumentation*
D008051:Lipid Bilayers