title: Liquid chromatography/tandem mass spectrometry for the determination of changrolin in rat plasma: application to a bioavailability study.
abstract: A sensitive and specific LC-CMS/MS method was developed for the quantification of changrolin, an anti-arrhythmic drug, in rat plasma using tiapride as internal standard. Liquid-liquid extraction was employed for sample preparation and analyzed using a multiple reaction monitoring mode with electrospray positive ionization source. The calibration curve for changrolin was linear over the range 5-1600 ng/mL with coefficients of correlation >0.99. The intra- and inter-batch precision was less than 8.6%, and accuracy ranged from 94.6% to 104.5%. This validated LC-MS/MS method was successfully applied to a bioavailability study of oral and intravenous administration of changrolin with 20mg/kg dosage in SD rats.
authors.affiliation: State Key Laboratory of Drug Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China.
authors.forename: Ding
authors.initials: D
authors.lastname: Yang
authors.forename: Chen
authors.initials: C
authors.lastname: Yu
authors.forename: Shuijun
authors.initials: S
authors.lastname: Li
authors.forename: Gangyi
authors.initials: G
authors.lastname: Liu
authors.forename: Youli
authors.initials: Y
authors.lastname: Lu
authors.forename: Yiping
authors.initials: Y
authors.lastname: Wang
mesh_terms: D000818:Animals; D000889:Anti-Arrhythmia Agents / Q000097:blood* / Q000493:pharmacokinetics; D001682:Biological Availability; D002138:Calibration; D002853:Chromatography, Liquid / Q000379:methods*; D011799:Quinazolines / Q000097:blood* / Q000493:pharmacokinetics; D051381:Rats; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D000889:Anti-Arrhythmia Agents; D011799:Quinazolines; C022128:changrolin