[Preparation of nanopaticles of SCF-CO2 extraction of Magnolia officinalis].
OBJECTIVE
To prepare nano-particles of SCF-CO2 extraction of Magnolia officinalis with rapid expansion of supercritical solution (RESS) and optimize the preparation procedure with orthogonal experiment.


METHODS
The preparation procedures were optimized by orthogonal test L9(3(4)) with the mean particle size and the amounts of total-phenol as indexes. Factors of the extraction pressure and temperature, diameter of nozzle, pre-expansion temperature were evaluated for their effects on preparation process.


RESULTS
The optimal preparation technology was as follows: the pressure was at 25 MPa and temperature was at 50 degrees C, the diameter of nozzle was 200 microm, the pre-expansion temperature was at 30 degrees C. The nano-particles solution was settled, of which the average particle size was 303.50 nm and the total-phenol contents was 0.091 g x L(-1), and the stability of the particle size kept well after 1 month of storage at low temperature and obturation the mean particle size.


CONCLUSIONS
The RESS method is applicable for preparing nanoparticles solution of SCF-CO2 extraction of M. officinalis at low operating temperature. It is simple processing, and no environmental pollution, and no residual solvent.
He
Shuai
S
Dept. of Pharmacy, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, China.
Zhang
Shouyao
S
Lei
Zhengjie
Z
Zhang
Zhongyi
Z
D002245:Carbon Dioxide / Q000737:chemistry*; D025924:Chromatography, Supercritical Fluid / Q000379:methods; D031566:Magnolia / Q000737:chemistry*; D053758:Nanoparticles; D010316:Particle Size; D010936:Plant Extracts / Q000737:chemistry; D012995:Solubility; D013678:Technology, Pharmaceutical; D013696:Temperature
D010936:Plant Extracts; D002245:Carbon Dioxide