In vivo developmental biology study using noninvasive multi-harmonic generation microscopy.
Morphological changes and complex developmental processes inside vertebrate embryos are difficult to observe noninvasively with millimeter-penetration and sub-micrometer-resolution at the same time. By using higher harmonic generation, including second and third harmonics, as the microscopic contrast mechanism, optical noninvasiveness can be achieved due to the virtual-level-transition characteristic. The intrinsic nonlinearity of harmonic generations provides optical sectioning capability while the selected 1230-nm near-infrared light source provides the deeppenetration ability. The complicated development within a ~1.5-mm thick zebrafish (Danio rerio) embryo from initial cell proliferation, gastrulation, to tissue formation can all be observed clearly in vivo without any treatment on the live specimen.
Chu
Shi-Wei
SW
Chen
Szu-Yu
SY
Tsai
Tsung-Han
TH
Liu
Tzu-Ming
TM
Lin
Cheng-Yung
CY
Tsai
Huai-Jen
HJ
Sun
Chi-Kuang
CK