title: [Study on preparation of berberine microemulsion and its absorption in intestine].
abstract: OBJECTIVE
  To prepare berberine microemulsion, and to investigate its properities and the absorption character in rat intestine in situ.
  
  
  METHODS
  The optimum formulation of the blank microemulsion selected by pseudo tertiary phase diagrams and the berberine microemulsion was prepared based on the blank microemulsion. The viscosity, conductance, refraction rate and particle size of berberine microemulsion were surveyed. An in situ rat perfusion method was used to investigate the intestinal absorption of berberine microemulsion. A UV method for determination of berberine in the intestinal flux was established.
  
  
  RESULTS
  The viscosity, conductance, refraction rate and particle size of berberine microemulsion were 2.11 cPas, 125.5 microomega, 1.363 and 24.0 nm, respectively. The absorption rate of berberine at the ileum was the best. The absorption of berberine microemulsion at the ileum was significantly higher than that of raw medicine (P < 0.01).
  
  
  CONCLUSIONS
  The microemulsion system might improve the absorption of berberine in the intestinal tract.
authors.affiliation: University of Science and Technology of China, Hefei 230027, China. guishy0520@yahoo.com.cn
authors.forename: Shuangying
authors.initials: S
authors.lastname: Gui
authors.forename: Lei
authors.initials: L
authors.lastname: Wu
authors.forename: Jun
authors.initials: J
authors.lastname: Pan
authors.forename: Zhiqiang
authors.initials: Z
authors.lastname: Wen
authors.forename: Weihua
authors.initials: W
authors.lastname: Kai
authors.forename: Jun
authors.initials: J
authors.lastname: Wang
mesh_terms: D000042:Absorption; D000279:Administration, Cutaneous; D000818:Animals; D001599:Berberine / Q000008:administration & dosage / Q000493:pharmacokinetics*; D016503:Drug Delivery Systems; D004355:Drug Stability; D005260:Female; D007082:Ileum / Q000378:metabolism; D007408:Intestinal Absorption / Q000187:drug effects*; D007413:Intestinal Mucosa / Q000378:metabolism*; D008297:Male; D010316:Particle Size; D051381:Rats; D017208:Rats, Wistar; D012867:Skin / Q000378:metabolism; D012869:Skin Absorption / Q000187:drug effects / Q000502:physiology; D012995:Solubility; D013678:Technology, Pharmaceutical / Q000379:methods
chemical_list: D001599:Berberine