Analysis on the guided bone augmentation in the rat calvarium using a microfocus computerized tomography analysis.
OBJECTIVE
Guided bone augmentation (GBA) in its most challenging form is the creation of new bone through the guidance of bone cells to an area beyond the original outer skeletal envelope. We used a microfocus computerized tomography (R_mCT) system to examine bone augmentation beyond the skeletal envelope in the rat calvarium.


METHODS
The calvarium was exposed and critical-size 5-mm defects were prepared bilaterally. Two resin caps were placed with or without hydroxyapatite (HA), and images of bone augmentation within the resin cap were obtained using R_mCT.


RESULTS
The experimental site showed bone regeneration beyond the skeletal envelope. Bone continuity was observed between the defect edge and HA. In contrast, no new bone formation was observed beyond the skeletal envelope at the control site. The bone volume increased significantly at the experimental site compared with the control site in a time-dependent manner.


CONCLUSIONS
The R_mCT system enabled the continuous observation of guided bone augmentation with graft materials in rat calvarium.
Kochi
Go
G
Division of Applied Oral Sciences, Nihon University School of Dentistry, Tokyo, Japan.
Sato
Shuichi
S
Fukuyama
Tomihisa
T
Morita
Chiharu
C
Honda
Kazuya
K
Arai
Yoshinori
Y
Ito
Koichi
K
D000818:Animals; D001861:Bone Regeneration / Q000187:drug effects*; D018786:Bone Substitutes / Q000494:pharmacology*; D048091:Guided Tissue Regeneration; D006882:Hydroxyapatites / Q000494:pharmacology*; D008297:Male; D051381:Rats; D011916:Rats, Inbred F344; D012886:Skull / Q000601:surgery; D014945:Wound Healing / Q000187:drug effects*; D055114:X-Ray Microtomography / Q000662:veterinary
D018786:Bone Substitutes; D006882:Hydroxyapatites