Prenatal ozone exposure abolishes stress activation of Fos and tyrosine hydroxylase in the nucleus tractus solitarius of adult rat.
Ozone (O3) is widely distributed in the environment, with high levels of air pollution. However, very few studies have documented the effects on postnatal development of O3 during pregnancy. The long-term effects of prenatal O3 exposure in rats (0.5 ppm 12 h/day from embryonic day E5 to E20) were evaluated in the adult nucleus tractus solitarius (NTS) regulating respiratory control. Neuronal response was assessed by Fos protein immunolabeling (Fos-IR), and catecholaminergic neuron involvement by tyrosine hydroxylase (TH) labeling (TH-IR). Adult offspring were analyzed at baseline and following immobilization stress (one hour, plus two hours' recovery); immunolabeling was observed by confocal microscopy. Prenatal O3 increased the baseline TH gray level per cell (p < 0.001). In contrast, the number of Fos-IR cells, Fos-IR/TH-IR colabeled cells and proportion of TH double-labeled with Fos remained unchanged. After stress, the TH gray level (p < 0.001), number of Fos-IR cells (p < 0.001) and of colabeled Fos-IR/TH-IR cells (p < 0.05) and percentage of colabeled Fos-IR/TH-IR neurons against TH-IR cells (p < 0.05) increased in the control group. In prenatal-O3 rats, immobilization stress abolished these increases and reduced the TH gray level (p < 0.05), indicating that prenatal O3 led to loss of adult NTS reactivity to stress. We conclude that long-lasting sequelae were detected in the offspring beyond the prenatal O3 exposure. Prenatal O3 left a print on the NTS, revealed by stress. Disruption of neuronal plasticity to new challenge might be suggested.
Boussouar
A
A
Université de Lyon, Lyon, F-69003, France.
Araneda
S
S
Hamelin
C
C
Soulage
C
C
Kitahama
K
K
Dalmaz
Y
Y
D000704:Analysis of Variance; D000818:Animals; D002452:Cell Count / Q000379:methods; D005260:Female; D008297:Male; D016761:Oncogene Proteins v-fos / Q000378:metabolism*; D010126:Ozone; D011247:Pregnancy; D011297:Prenatal Exposure Delayed Effects / Q000139:chemically induced* / Q000503:physiopathology; D051381:Rats; D012149:Restraint, Physical / Q000379:methods; D017552:Solitary Nucleus / Q000378:metabolism*; D013315:Stress, Psychological / Q000201:enzymology / Q000473:pathology*; D014446:Tyrosine 3-Monooxygenase / Q000378:metabolism*
D016761:Oncogene Proteins v-fos; D010126:Ozone; D014446:Tyrosine 3-Monooxygenase