title: Impact of pancreatic cold preservation on rat islet recovery and function.
abstract: BACKGROUND
  Islet transplantation success depends on the number and quality of islets transplanted. This study aimed at exploring the molecular mechanisms associated with cold pancreas preservation and their impact on islet cell survival and function.
  
  
  METHODS
  Rat pancreata were stored in cold University of Wisconsin preservation solution for short (3 hr; control) or long (18 hr) cold ischemia times (CIT).
  
  
  RESULTS
  Pancreata exposed to long CIT yielded lower islet numbers and showed reduced cellular viability; isolated islets displayed higher levels of phosphorylated stress-activated protein kinase (c-jun N-terminal Kinase and Mitogen-Activated Protein Kinase-p38), and chemokine (C-C) ligand-3, and lower levels of vascular endothelial growth factor, interleukins (IL)-9 and IL-10. Islets obtained from long-CIT pancreata were functionally impaired after transplantation. Differential proteomic expression in pancreatic tissue after CIT included increased eukaryotic translation elongation factor-1-alpha-1 (apoptosis related) and reduced Clade-B (serine protease inhibitor).
  
  
  CONCLUSIONS
  Our study indicates that cold ischemia stimulates inflammatory pathways (chemokine (c-c)ligand-3, phosphorylation of c-jun N-terminal Kinase and mitogen-activated protein kinase-p38, and eukaryotic translation elongation factor-1-alpha-1) and decreases repair/cytoprotective pathways (IL-10, vascular endothelial growth factor, and Clade-B), all of which may negatively affect the quality and mass of islets obtained from a donor pancreas.
authors.affiliation: Cell Transplant Center, Diabetes Research Institute, University of Miami-Leonard M. Miller School of Medicine, Miami, FL 33136, USA. APileggi@med.miami.edu
authors.forename: Antonello
authors.initials: A
authors.lastname: Pileggi
authors.forename: Melina M
authors.initials: MM
authors.lastname: Ribeiro
authors.forename: Anthony R
authors.initials: AR
authors.lastname: Hogan
authors.forename: R Damaris
authors.initials: RD
authors.lastname: Molano
authors.forename: Lorenzo
authors.initials: L
authors.lastname: Cobianchi
authors.forename: Hirohito
authors.initials: H
authors.lastname: Ichii
authors.forename: Jennifer
authors.initials: J
authors.lastname: Embury
authors.forename: Luca
authors.initials: L
authors.lastname: Inverardi
authors.forename: Alessia
authors.initials: A
authors.lastname: Fornoni
authors.forename: Camillo
authors.initials: C
authors.lastname: Ricordi
authors.forename: Ricardo L
authors.initials: RL
authors.lastname: Pastori
mesh_terms: D000241:Adenosine; D000493:Allopurinol; D000818:Animals; D002470:Cell Survival; D003080:Cold Temperature; D016207:Cytokines / Q000378:metabolism; D005978:Glutathione; D006085:Graft Survival; D007328:Insulin; D007515:Islets of Langerhans / Q000166:cytology* / Q000473:pathology / Q000502:physiology; D016381:Islets of Langerhans Transplantation / Q000502:physiology; D008297:Male; D051379:Mice; D008819:Mice, Nude; D009926:Organ Preservation / Q000379:methods*; D019188:Organ Preservation Solutions; D010179:Pancreas / Q000166:cytology*; D010766:Phosphorylation; D011494:Protein Kinases / Q000378:metabolism; D011887:Raffinose; D051381:Rats; D011917:Rats, Inbred Lew; D020858:Tissue and Organ Harvesting / Q000379:methods
chemical_list: D016207:Cytokines; D007328:Insulin; D019188:Organ Preservation Solutions; C055206:University of Wisconsin-lactobionate solution; D000493:Allopurinol; D011494:Protein Kinases; D005978:Glutathione; D000241:Adenosine; D011887:Raffinose