Effects of asbestiform antigorite on human alveolar epithelial A549 cells: a morphological and immunohistochemical study.
The purpose of the study was to investigate the biological risk of asbestiform antigorite, which is a fibrous variety of antigorite, one of the natural mineral fibres of the serpentine group to which asbestos chrysotile belongs. Asbestiform antigorite is very abundant and commonly found associated with asbestos chrysotile in serpentinites, a kind of rock outcropping present in many geographical locations worldwide. In this study we evaluated the morphological, immunohistochemical and functional effects of antigorite fibres in alveolar epithelial cancer cells (A549), a standardized human cell line currently used as a model to study cytotoxicity induced by pharmacological agents. The antigorite fibres were identified and characterized morphologically and chemically by X-ray powder diffractometry, transmission and scanning electron microscopy, both with annexed energy dispersive spectrometry. The effects of 50 microg/ml of antigorite in A549 lung cells treated at 24 and 48 h resulted in increased synthesis of VEGF, Cdc42 and beta-catenin that represent potential risks for cancer development. Phalloidin labelling showed an irregular distribution of filamentous actin resulting from antigorite contact. Our studies indicate potential cellular toxicity of antigorite in vivo, providing the opportunity to elucidate the effect of asbestos on cancer induction and possible modes of therapy.
Pugnaloni
Armanda
A
Department of Molecular Pathology and Innovative Therapies Histology Section, Marche Polytechnic University, Università Politecnica delle Marche, via Tronto n 10/A, I-60020 Torrette Ancona, Italy. armanda.pugnaloni@univpm.it
Giantomassi
Federica
F
Lucarini
Guendalina
G
Capella
Silvana
S
Belmonte
Monica Mattioli
MM
Orciani
Monia
M
Belluso
Elena
E
D008841:Actin Cytoskeleton / Q000378:metabolism*; D017632:Asbestos, Serpentine / Q000633:toxicity*; D002460:Cell Line; D002470:Cell Survival; D002471:Cell Transformation, Neoplastic; D004847:Epithelial Cells / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D006801:Humans; D011650:Pulmonary Alveoli / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism*; D014961:X-Ray Diffraction; D051176:beta Catenin / Q000378:metabolism*; D020764:cdc42 GTP-Binding Protein / Q000378:metabolism*
D017632:Asbestos, Serpentine; D042461:Vascular Endothelial Growth Factor A; D051176:beta Catenin; D020764:cdc42 GTP-Binding Protein