[Expression of recombinant rubella virus E1 protein and initial application for detecting of antibody].
OBJECTIVE
To apply recombinant rubella virus envelope protein-1 (E1) to detect human rubella virus IgG antibody.


METHODS
Rubella virus E1 protein was expressed in E. coli, purified E1 protein was used as the antigen for the detecting of anti rubella in human sera in the way of enzyme linked Immunosorbant assay (ELISA).


RESULTS
The antigenicity of the recombinant protein was checked by WHO rubella sera panel. We detected 200 sera samples, which came from Guangxi Guilin. 93% of these samples were positive.


CONCLUSIONS
The antigenicity of recombinant E1 is a satisfied candidate antigen for the detecting of human rubella virus antibody. The prevalence of anti rubella virus IgG in Guangxi is 93%. It is at the some level compared with other provinces in China.
Yi
Yao
Y
Institute of Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 100052, China.
Guo
Min-zhuo
MZ
Yu
Tao
T
Xu
Wen-bo
WB
Yang
Jin-ye
JY
Chen
Si-yong
SY
D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis* / Q000276:immunology; D002681:China; D002522:Chlorocebus aethiops; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics; D015870:Gene Expression; D005822:Genetic Vectors; D006801:Humans; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology*; D012412:Rubella virus / Q000235:genetics* / Q000276:immunology; D014709:Vero Cells / Q000821:virology; D014759:Viral Envelope Proteins / Q000235:genetics / Q000378:metabolism*
D000914:Antibodies, Viral; D011994:Recombinant Proteins; D014759:Viral Envelope Proteins