title: [Matrix metalloproteinase 8 and 9 regulations of polymorphonuclear leukocytes stimulated by Porphyromonas gingivalis with different fimA genotypes].
abstract: OBJECTIVE
  To investigate the pathogenicity of matrix metalloproteinase 8, 9 (MMP-8, MMP-9) regulations of polymorphonuclear leukocytes (PMNs) by challenge of Porphyromonas gingivalis (P. gingivalis) with different fimA genotypes.
  
  
  METHODS
  The studies mainly adopt the isopycnic sedimentation separation to separate the PMNs from human peripheral blood. P. gingivalis ATCC 33277 (type I), WCSP 115 (type II), WCSP 1.5 (type III), W83 (type IV), WCSP 559 (type IV) were assessed for their inductions of MMP-8, MMP-9 expression in PMNs. MMP-8, MMP-9 protein levels in culture supernatant were determined by ELISA at different time intervals (5 min, 30 min, 1 h, 2 h) following continuous co-culture of bacteria with PMNs.
  
  
  RESULTS
  MMP-8 and MMP-9 protein levels produced by PMNs co-culture with the I fimA-IV fimA P. gingivalis were significantly stronger than unsimulated group. The velocity and quantity of MMP-8 produced by PMNs co-culture with the II fimA P. gingivalis and IV fimA P. gingivalis were more than III fimA, IVfimA P. gingivalis. The MMP-9 protein levels produced by PMNs co-culture with the I fimA, II fimA, IV fimA P. gingivalis was significantly stronger than III fimA and IV fimA P. gingivalis.
  
  
  CONCLUSIONS
  II fimA and IV fimA P. gingivalis have stronger pathogenicity relatively, which indicate that fimA genotype is associated with pathogenesis of P. gingivalis.
authors.affiliation: State Key Laboratory of Oral Diseases, Sichuan University, Chengdu 610041, China.
authors.forename: Yu-Ling
authors.initials: YL
authors.lastname: Ouyang
authors.forename: Ya-Fei
authors.initials: YF
authors.lastname: Wu
authors.forename: Lei
authors.initials: L
authors.lastname: Zhao
authors.forename: Xiao-Rong
authors.initials: XR
authors.lastname: Xiao
authors.forename: Jing-Yi
authors.initials: JY
authors.lastname: Zhang
authors.forename: Xiao-Yu
authors.initials: XY
authors.lastname: Li
mesh_terms: D018920:Coculture Techniques; D036781:Fimbriae Proteins; D005838:Genotype; D006801:Humans; D020784:Matrix Metalloproteinase 8; D009504:Neutrophils; D016966:Porphyromonas gingivalis
chemical_list: D036781:Fimbriae Proteins; D020784:Matrix Metalloproteinase 8