title: Reliability of Pseudomonas aeruginosa semi-automated rep-PCR genotyping in various epidemiological situations.
abstract: The purpose of this study was to evaluate the possibility of using a semi-automated repetitive DNA sequences-based polymerase chain reaction (rep-PCR) for typing Pseudomonas aeruginosa isolates. rep-PCR profiles obtained by the DiversiLab system of 84 P. aeruginosa isolates from distinct epidemiological situations were obtained. rep-PCR groupings were in good agreement with the origin of these isolates. Linked rep-PCR profiles were observed for isolates recovered from a same family of cystic fibrosis (CF) patients, for the etiological agents of clustered cases of nosocomial infections, and for some isolates recovered from a same hospital room. rep-PCR and pulsed-field gel electrophoresis SpeI restricted genomic DNA (PFGE-SpeI) profiles were compared. In a few instances, rep-PCR revealed genetic divergences among isolates of a same group of PFGE-SpeI profiles. These divergences could reflect genetic drifts among closely related isolates, as illustrated by those observed between clinical and environmental isolates of a same group of PFGE-SpeI profiles. The interpretation of such differences will require further studies, but the rep-PCR analysis of P. aeruginosa diversity appeared to be an appropriate method to investigate infra-specific genetic relatedness.
authors.affiliation: Université de Lyon, Lyon, France. anne.jordheim@recherche.univ-lyon1.fr
authors.forename: A
authors.initials: A
authors.lastname: Doléans-Jordheim
authors.forename: B
authors.initials: B
authors.lastname: Cournoyer
authors.forename: E
authors.initials: E
authors.lastname: Bergeron
authors.forename: J
authors.initials: J
authors.lastname: Croizé
authors.forename: H
authors.initials: H
authors.lastname: Salord
authors.forename: J
authors.initials: J
authors.lastname: André
authors.forename: M-A
authors.initials: MA
authors.lastname: Mazoyer
authors.forename: F N R
authors.initials: FN
authors.lastname: Renaud
authors.forename: J
authors.initials: J
authors.lastname: Freney
mesh_terms: D001331:Automation / Q000379:methods*; D015373:Bacterial Typing Techniques / Q000379:methods*; D016172:DNA Fingerprinting / Q000379:methods*; D004269:DNA, Bacterial / Q000235:genetics; D006801:Humans; D020071:Interspersed Repetitive Sequences; D017720:Molecular Epidemiology / Q000379:methods; D016133:Polymerase Chain Reaction / Q000379:methods*; D011552:Pseudomonas Infections / Q000453:epidemiology / Q000382:microbiology*; D011550:Pseudomonas aeruginosa / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification
chemical_list: D004269:DNA, Bacterial