title: A leukemic plasmacytoid dendritic cell line, PMDC05, with the ability to secrete IFN-alpha by stimulation via Toll-like receptors and present antigens to naïve T cells.
abstract: We established a plasmacytoid dendritic cell (pDC) line (PMDC05) from leukemia cells of pDC leukemia. PMDC05 cells were positive for CD4, CD56, CD33, HLA-DR, CD123 (IL-3Ralpha) and CD86 in the absence of lineage markers. mRNA of TLR1, TLR2, TLR4, TLR7 and TLR9 was clearly expressed and among these TLRs, TLR7 was prominent. Production of IFN-alpha and IL-12 in PMDC05 was enhanced by the stimulation with CpG-A and LPS, respectively. PMDC05 possessed a considerable antigen presenting ability, which was enhanced by culturing with IL3, influenza virus or LPS. PMDC05 could be a useful tool for investigating the pathophysiology of pDCL.
authors.affiliation: Laboratory of Hematology and Oncology, Graduate School of Health Sciences, Niigata University, Niigata, Japan. naritami@clg.niigata-u.ac.jp
authors.forename: Miwako
authors.initials: M
authors.lastname: Narita
authors.forename: Norihiro
authors.initials: N
authors.lastname: Watanabe
authors.forename: Akie
authors.initials: A
authors.lastname: Yamahira
authors.forename: Shigeo
authors.initials: S
authors.lastname: Hashimoto
authors.forename: Nozomi
authors.initials: N
authors.lastname: Tochiki
authors.forename: Anri
authors.initials: A
authors.lastname: Saitoh
authors.forename: Masami
authors.initials: M
authors.lastname: Kaji
authors.forename: Takeshi
authors.initials: T
authors.lastname: Nakamura
authors.forename: Tatsuo
authors.initials: T
authors.lastname: Furukawa
authors.forename: Ken
authors.initials: K
authors.lastname: Toba
authors.forename: Ichiro
authors.initials: I
authors.lastname: Fuse
authors.forename: Yoshifusa
authors.initials: Y
authors.lastname: Aizawa
authors.forename: Masuhiro
authors.initials: M
authors.lastname: Takahashi
mesh_terms: D000941:Antigens / Q000276:immunology; D001483:Base Sequence; D045744:Cell Line, Tumor; D017931:DNA Primers; D003713:Dendritic Cells / Q000276:immunology / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D016898:Interferon-alpha / Q000378:metabolism*; D007621:Karyotyping; D007952:Leukemia, Plasma Cell / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013601:T-Lymphocytes / Q000276:immunology*; D051193:Toll-Like Receptors / Q000502:physiology*
chemical_list: D000941:Antigens; D017931:DNA Primers; D016898:Interferon-alpha; D051193:Toll-Like Receptors