title: [AOTF-near infrared spectroscopy for determining tanshinone II A in returning extraction process of Salvia miltiorrhiza].
abstract: OBJECTIVE
  To explore a method on-line determining the contents of tanshinone II A in returning extraction process of Salvia miltiorrhiza by AOTF-Near infrared spectroscopy.
  
  
  METHODS
  Firstly, the sample was collected in the extraction process of Salvia miltiorrhiza. Then HPLC was used as a reference method to determine the contents of tanshinone II A in the sample. Multivariate calibration models based on PLS1 algorithm were developed to correlate the spectra and the corresponding values determined by the reference methods.
  
  
  RESULTS
  RMSECV of the models for tanshinone II A was 0.0092. The correlation coefficients of the calibration models was 0.9918. External validation with external validation samples proved that the relative deviation was 5.74%.
  
  
  CONCLUSIONS
  AOTF-NIRS can be used in the determination of tanshinone II A in returning extraction process of Salvia miltiorrhiza. The method is rapid, accurate and non-destructive, and can be applied for process analysis and quality control of Chinese medicine manufacturing process.
authors.affiliation: ShanDong Medical College, Jinan 250002, China. weihong204@163.com
authors.forename: Wei-hong
authors.initials: WH
authors.lastname: Wu
authors.forename: Ning
authors.initials: N
authors.lastname: Wang
authors.forename: Shao-song
authors.initials: SS
authors.lastname: Cai
authors.forename: Hong
authors.initials: H
authors.lastname: Wei
mesh_terms: D045784:Abietanes; D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D016018:Least-Squares Analysis; D008962:Models, Theoretical; D010616:Phenanthrenes / Q000032:analysis* / Q000302:isolation & purification; D011786:Quality Control; D015203:Reproducibility of Results; D027561:Salvia miltiorrhiza / Q000737:chemistry*; D019265:Spectroscopy, Near-Infrared
chemical_list: D045784:Abietanes; D004365:Drugs, Chinese Herbal; D010616:Phenanthrenes; C021751:tanshinone