title: Novel fucosylated biomarkers for the early detection of hepatocellular carcinoma.
abstract: Changes in glycosylation, most notably fucosylation, have been associated with the development of hepatocellular carcinoma (HCC). In this report, the levels of fucosylated kininogen (Fc-Kin) and fucosylated alpha-1-antitrypsin were analyzed individually and in combination with the currently used marker, alpha-fetoprotein, and a previously identified biomarker, Golgi protein 73 (GP73), for the ability to distinguish between a diagnosis of cirrhosis and HCC. This analysis was done on serum from 113 patients with cirrhosis and 164 serum samples from patients with cirrhosis plus HCC. The levels of Fc-Kin and fucosylated alpha-1-antitrypsin were significantly higher in patients with HCC compared with those with cirrhosis (P < 0.0001). Greatest performance was achieved through the combination of Fc-Kin, alpha-fetoprotein, and GP73, giving an optimal sensitivity of 95%, a specificity of 70%, and an area under the receiver operating characteristic of 0.94. In conclusion, the altered glycosylation of serum glycoproteins can act as potential biomarkers of primary HCC when used independently or in combination with other markers of HCC.
authors.affiliation: Department of Microbiology and Immunology, Drexel Institute for Biotechnology and Virology, Drexel University College of Medicine, Doylestown, Pennsylvania, USA.
authors.forename: Mengjun
authors.initials: M
authors.lastname: Wang
authors.forename: Ronald E
authors.initials: RE
authors.lastname: Long
authors.forename: Mary Ann
authors.initials: MA
authors.lastname: Comunale
authors.forename: Omer
authors.initials: O
authors.lastname: Junaidi
authors.forename: Jorge
authors.initials: J
authors.lastname: Marrero
authors.forename: Adrian M
authors.initials: AM
authors.lastname: Di Bisceglie
authors.forename: Timothy M
authors.initials: TM
authors.lastname: Block
authors.forename: Anand S
authors.initials: AS
authors.lastname: Mehta
mesh_terms: D019540:Area Under Curve; D014408:Biomarkers, Tumor / Q000097:blood*; D015153:Blotting, Western; D006528:Carcinoma, Hepatocellular / Q000097:blood* / Q000378:metabolism / Q000473:pathology; D055088:Early Detection of Cancer; D005260:Female; D006031:Glycosylation; D006801:Humans; D007118:Immunoassay / Q000379:methods; D007704:Kininogens / Q000378:metabolism; D008113:Liver Neoplasms / Q000097:blood* / Q000378:metabolism / Q000473:pathology; D008297:Male; D009367:Neoplasm Staging; D012372:ROC Curve; D012680:Sensitivity and Specificity; D000515:alpha 1-Antitrypsin / Q000378:metabolism
chemical_list: D014408:Biomarkers, Tumor; D007704:Kininogens; C043392:SERPINA1 protein, human; D000515:alpha 1-Antitrypsin