title: Does imidacloprid seed-treated maize have an impact on honey bee mortality?
abstract: Beekeepers suspected maize, Zea mays L., treated with imidacloprid to result in substantial loss of honey bee (Hymenoptera: Apidae) colonies in Belgium. The objective of this study was to investigate the potential impact of maize grown from imidacloprid-treated seeds on honey bee mortality. A survey of 16 apiaries was carried out, and all maize fields treated or not with imidacloprid were located within a radius of 3,000 m around the observed apiaries. Samples of honey, beeswax, and bees were collected in three colonies per apiary and analyzed for pesticide contain by liquid chromatography-tandem mass spectrometry and gas chromatography-tandem mass spectrometry. We first found a significant correlation between the number of colonies per apiary and the mortality rates in an apiary. In addition, this mortality rate was inversely correlated with the surface of maize fields treated and not with imidacloprid, suggesting that this pesticide do not interact with bees' fitness. Moreover, a very large number of our samples contained acaricides either prohibited or ineffective against Varroa destructor (Anderson & Trueman) (Acari: Varroidae), suggesting that the treatment methods used by the beekeepers to be inadequate for mite control. Our results support the hypothesis that imidacloprid seed-treated maize has no negative impact on honey bees.
authors.affiliation: Department of Functional and Evolutionary Entomology, FUSAGx, 5030 Gembloux, Belgium. nguyen.b@fsagx.ac.be
authors.forename: B K
authors.initials: BK
authors.lastname: Nguyen
authors.forename: C
authors.initials: C
authors.lastname: Saegerman
authors.forename: C
authors.initials: C
authors.lastname: Pirard
authors.forename: J
authors.initials: J
authors.lastname: Mignon
authors.forename: J
authors.initials: J
authors.lastname: Widart
authors.forename: B
authors.initials: B
authors.lastname: Thirionet
authors.forename: F J
authors.initials: FJ
authors.lastname: Verheggen
authors.forename: D
authors.initials: D
authors.lastname: Berkvens
authors.forename: E
authors.initials: E
authors.lastname: De Pauw
authors.forename: E
authors.initials: E
authors.lastname: Haubruge
mesh_terms: D000818:Animals; D001516:Bees / Q000187:drug effects*; D006722:Honey / Q000032:analysis; D007093:Imidazoles / Q000737:chemistry / Q000494:pharmacology*; D007306:Insecticides / Q000737:chemistry / Q000494:pharmacology*; D000073943:Neonicotinoids; D009574:Nitro Compounds / Q000737:chemistry / Q000494:pharmacology*; D010573:Pesticide Residues / Q000737:chemistry; D012639:Seeds; D014885:Waxes / Q000737:chemistry; D003313:Zea mays
chemical_list: D007093:Imidazoles; D007306:Insecticides; D000073943:Neonicotinoids; D009574:Nitro Compounds; D010573:Pesticide Residues; D014885:Waxes; C038228:beeswax; C082359:imidacloprid