Electrochemical assay of superoxide based on biomimetic enzyme at highly conductive TiO2 nanoneedles: from principle to applications in living cells.
A novel strategy for extremely durable, reliable, and selective in situ real-time determination of superoxide anion (O(2) (-)) based on direct electron transfer of a biomimetic superoxide dismutase (SOD), Mn(3)(PO(4))(2), at highly conductive TiO(2) nanoneedles is reported for the first time, and is further exploited for the determination of O(2) (-) from living normal and cancer ovary cells directly adhered onto the modified surface, suggesting that O(2) (-) might be proposed as a biomarker of cancer.
Luo
Yongping
Y
Department of Chemistry, Tongji University, Shanghai, 200092, China.
Tian
Yang
Y
Rui
Qi
Q
D002460:Cell Line; D004563:Electrochemistry / Q000379:methods*; D006801:Humans; D018716:Molecular Mimicry; D049329:Nanostructures; D013482:Superoxide Dismutase / Q000737:chemistry*; D013481:Superoxides / Q000032:analysis*; D014025:Titanium / Q000737:chemistry*
D013481:Superoxides; C009495:titanium dioxide; D014025:Titanium; D013482:Superoxide Dismutase