title: Antimicrobial and antioxidant activity of the essential oil and methanol extract of Nepeta cataria.
abstract: Catnip (Nepeta cataria) is an important medicinal herb belonging to the mint family, Lamiaceae. In this study, the in vitro antimicrobial and antioxidant activities of the essential oil and methanol extract from Nepeta cataria, and its essential oil composition were investigated. The essential oil, which has 4aalpha,7alpha,7abeta-nepetalactone (70.4%), 4aalpha,7alpha,7abeta-nepetalactone (6.0%), thymol (2.3%), and 4aalpha,7alpha, 7abeta3-nepetalactone (2.5%), as main components, exhibited activity against eleven bacteria, and twelve fungi and a yeast, C. albicans; with Minimum Inhibitory Concentrations (MIC) values ranging from 12.50 to 250 microl/ml; the methanol extract showed weaker activity. The samples were also subjected to a screening for their possible antioxidant activities by using 2.2-diphenyl-1-picrylhydrazyl (DPPH) and beta-carotene/linoleic acid assays. In DPPH assay, the extract showed slight antioxidant activity whereas the essential oil remained inactive. In the latter case, both the extract and the essential oil exerted weak activity having inhibiton ratios of linoleic acid oxidation at 16.4% and 27.0%, respectively. The weak antioxidative nature of the extract could be attributed to the low phenolic content, estimated as gallic acid equivalent at 22.6 +/- 2.07 microg/ml or 2.26%. In both systems, antioxidant capacity of BHT was determined in parallel experiments.
authors.affiliation: Health Services Vocational Training School, Ataturk University, Yenisehir-Erzurum, 25070, Turkey.
authors.forename: Ahmet
authors.initials: A
authors.lastname: Adiguzel
authors.forename: Hakan
authors.initials: H
authors.lastname: Ozer
authors.forename: Munevver
authors.initials: M
authors.lastname: Sokmen
authors.forename: Medine
authors.initials: M
authors.lastname: Gulluce
authors.forename: Atalay
authors.initials: A
authors.lastname: Sokmen
authors.forename: Hamdullah
authors.initials: H
authors.lastname: Kilic
authors.forename: Fikrettin
authors.initials: F
authors.lastname: Sahin
authors.forename: Ozlem
authors.initials: O
authors.lastname: Baris
mesh_terms: D000890:Anti-Infective Agents / Q000494:pharmacology*; D000975:Antioxidants / Q000378:metabolism*; D001419:Bacteria / Q000187:drug effects; D001713:Biphenyl Compounds / Q000378:metabolism; D000081004:Cyclopentane Monoterpenes; D003517:Cyclopentanes / Q000494:pharmacology; D005658:Fungi / Q000187:drug effects; D008401:Gas Chromatography-Mass Spectrometry; D019787:Linoleic Acid / Q000378:metabolism; D000432:Methanol / Q000737:chemistry; D008826:Microbial Sensitivity Tests; D027521:Nepeta; D010851:Picrates / Q000378:metabolism; D010936:Plant Extracts / Q000378:metabolism / Q000494:pharmacology*; D010938:Plant Oils / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D011753:Pyrones / Q000494:pharmacology
chemical_list: D000890:Anti-Infective Agents; D000975:Antioxidants; D001713:Biphenyl Compounds; D000081004:Cyclopentane Monoterpenes; D003517:Cyclopentanes; D010851:Picrates; D010936:Plant Extracts; D010938:Plant Oils; D011753:Pyrones; C115644:nepetalactone; D019787:Linoleic Acid; C004931:1,1-diphenyl-2-picrylhydrazyl; D000432:Methanol