Atomic-level mapping of antibody epitopes on a GPCR.
Epitopes that define the immunodominant regions of conformationally complex integral membrane proteins have been difficult to reliably delineate. Here, a high-throughput approach termed shotgun mutagenesis was used to map the binding epitopes of five different monoclonal antibodies targeting the GPCR CCR5. The amino acids, and in some cases the atoms, that comprise the critical contact points of each epitope were identified, defining the immunodominant structures of this GPCR and their physicochemistry.
Paes
Cheryl
C
Integral Molecular, Inc., 3701 Market Street, Fourth Floor, Philadelphia, Pennsylvania 19104, USA.
Ingalls
Jada
J
Kampani
Karan
K
Sulli
Chidananda
C
Kakkar
Esha
E
Murray
Meredith
M
Kotelnikov
Valery
V
Greene
Tiffani A
TA
Rucker
Joseph B
JB
Doranz
Benjamin J
BJ
D000911:Antibodies, Monoclonal / Q000737:chemistry / Q000276:immunology*; D018604:Epitope Mapping / Q000379:methods*; D005455:Fluorescent Antibody Technique / Q000379:methods; D016056:Immunodominant Epitopes / Q000032:analysis*; D008958:Models, Molecular; D016296:Mutagenesis; D016133:Polymerase Chain Reaction / Q000379:methods; D019713:Receptors, CCR5 / Q000235:genetics / Q000276:immunology*
D000911:Antibodies, Monoclonal; D016056:Immunodominant Epitopes; D019713:Receptors, CCR5