title: Mutation L437P in the 2B domain of keratin 1 causes diffuse palmoplantar keratoderma in a Chinese pedigree.
abstract: Diffuse palmoplantar keratoderma (DPPK) is an autosomal dominant genodermatosis characterized by uniform hyperkeratosis of the palm and sole epidermis. This disorder can be caused by mutations in the genes keratin 1, keratin 9, keratin 16, desmoglein 1 and plakoglobin. Here we present a DPPK Chinese pedigree and identify the aetiology as a novel missense mutation, L437P, located in a highly conserved helix motif in domain 2B of KRT1. Functional analysis shows that overexpression of the L437P mutant in cultured cells leads to abnormal intermediate filament networks and filament aggregation. This gain-of-function mutation highlights the role of domain 2B in mediating filament assembly.
authors.affiliation: State Key Laboratory of Medical Genetics of China, Xiangya Hospital, Central South University, Changsha, Hunan, China.
authors.forename: X P
authors.initials: XP
authors.lastname: Liu
authors.forename: J
authors.initials: J
authors.lastname: Ling
authors.forename: H
authors.initials: H
authors.lastname: Xiong
authors.forename: X L
authors.initials: XL
authors.lastname: Shi
authors.forename: X
authors.initials: X
authors.lastname: Sun
authors.forename: Q
authors.initials: Q
authors.lastname: Pan
authors.forename: Z M
authors.initials: ZM
authors.lastname: Hu
authors.forename: L Q
authors.initials: LQ
authors.lastname: Wu
authors.forename: D S
authors.initials: DS
authors.lastname: Liang
authors.forename: Z G
authors.initials: ZG
authors.lastname: Long
authors.forename: H P
authors.initials: HP
authors.lastname: Dai
authors.forename: J H
authors.initials: JH
authors.lastname: Xia
authors.forename: K
authors.initials: K
authors.lastname: Xia
mesh_terms: D002681:China; D005260:Female; D020022:Genetic Predisposition to Disease / Q000208:ethnology / Q000235:genetics; D005838:Genotype; D006801:Humans; D007382:Intermediate Filaments / Q000473:pathology; D053556:Keratin-1 / Q000235:genetics*; D015776:Keratoderma, Palmoplantar, Diffuse / Q000208:ethnology* / Q000235:genetics* / Q000473:pathology; D008297:Male; D020125:Mutation, Missense / Q000235:genetics*; D010375:Pedigree; D010641:Phenotype
chemical_list: D053556:Keratin-1