Design of a novel interleukin-13 antagonist from analysis of informational structure.
Interleukin-13 (IL-13) is one of the cytokines involved in the development of Th2-type immune response. It plays an important role in the pathogenesis of asthma and other allergic diseases. Two deletion forms of IL-13 were constructed on a basis of informational structure analysis and expressed in E. coli cells. They were found to differ in ability to stimulate proliferation of TF-1 cell line. Deletion variant 146 (DV146) completely lacks such activity, whereas DV148 provides about 50% of the proliferation stimulation. The simultaneous addition of DV146 with full-length IL-13 suppresses proliferation depending on the concentration of the deletion form. Thus, the designed protein acts as an antagonist of IL-13.
Nekrasov
A N
AN
Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, 117997, Russia. alexei_nekrasov@mail.ru
Petrovskaya
L E
LE
Toporova
V A
VA
Kryukova
E A
EA
Rodina
A V
AV
Moskaleva
E Yu
EY
Kirpichnikov
M P
MP
D000595:Amino Acid Sequence; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D015195:Drug Design; D006801:Humans; D018793:Interleukin-13 / Q000037:antagonists & inhibitors* / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D008968:Molecular Conformation; D008969:Molecular Sequence Data; D017384:Sequence Deletion
D018793:Interleukin-13