title: A biphasic malignant mesothelioma of the peritoneum and pleura in a horse.
abstract: This report describes the macroscopic, histologic, immunohistologic and ultrastructural characteristics ofa biphasic malignant mesothelioma in the peritoneal and pleural cavity of a 13-year-old Icelandic pony mare, which exhibited recurrent ascites clinically. Immunohistology was performed employing multiple monoclonal antibodies against cytokeratins (CK) and vimentin. The ultrastructural examination included the quantitative evaluation of the length to diameter ratio of the microvilli. Post mortem examination revealed a severe ascites and hydrothorax. The serosal surfaces of the peritoneum and pleura displayed poorly-demarcated, multifocal to coalescing laminar masses and small nodules. Histology revealed a bimorphic mass consisting of spindle-shaped cells and microcystic epithelioid areas. A transcoelomic and local invasive growth pattern as well as lymph node metastases were noticed. Immunohistology revealed a strong expression of CK. Though a low and moderate expression of CK5/6 and CK20 was present, respectively, CK7 and CK10-antigens were lacking. Ultrastructurally, the epithelioid mesothelioma cells displayed long microvilli, cytoplasmic tonofilaments, and desmosomes. Quantitative evaluation of the length to diameter ratio of the 10 longest microvilli revealed a mean value of approximately 16.2. Summarized, this report described the case of a malignant biphasic mesothelioma with an atypical CK20 expression but a characteristic ultrastructural morphology including long microvilli.
authors.affiliation: Institut für Pathologie, Stiftung Tierärztliche Hochschule Hannover, Germany. reiner.ulrich@tiho-hannover.de
authors.forename: Reiner
authors.initials: R
authors.lastname: Ulrich
authors.forename: Maja
authors.initials: M
authors.lastname: Eydner
authors.forename: Anne
authors.initials: A
authors.lastname: Grün
authors.forename: Julia
authors.initials: J
authors.lastname: Haydn
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Baumgärtner
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D017809:Fatal Outcome; D006734:Horse Diseases / Q000473:pathology*; D006736:Horses; D007150:Immunohistochemistry / Q000662:veterinary; D007633:Keratins / Q000276:immunology; D008297:Male; D008654:Mesothelioma / Q000473:pathology / Q000648:ultrastructure / Q000662:veterinary*; D008871:Microvilli / Q000648:ultrastructure; D010534:Peritoneal Neoplasms / Q000473:pathology / Q000648:ultrastructure / Q000662:veterinary*; D010997:Pleural Neoplasms / Q000473:pathology / Q000648:ultrastructure / Q000662:veterinary*; D014746:Vimentin / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal; D014746:Vimentin; D007633:Keratins