title: The establishment and clinical appliance of technique of mucosa marking targeting biopsy.
abstract: OBJECTIVE
  Mucosa marking targeting biopsy (MTB) technique has been tested and verified in animal model. For multi-focal atrophic gastritis, random biopsy may miss the point of atrophy, intestinal metaplasia or dysplasia. In order to monitor chronic atrophic gastritis on gastroscopy, get the proper biopsy sample is very important.
  
  
  METHODS
  Fifty-three atrophic gastritis patients were enrolled prospectively in this study. India ink was tattooed at five points of stomach. Endoscopy was repeated at 3, 9, 15 and 24 months.
  
  
  RESULTS
  Tattoos (96.2%) produced with 1:10 concertrations were visible with a good to excellent tattoo persistence at 3 months. Only two patients (3.8%) had poor tattoo persistence and were retattooed at 3-months interval. Tattoos did not disappear in patients who repeated for more than two gastroscopies at 24 months or even long. There were no complications related to India ink tattooing including abdominal pain, bleeding or perforation. At follow-up gastroscopy, no ulcers, inflammation, break in the mucosa, or pain was noted.
  
  
  CONCLUSIONS
  Clinical use of MTB technique is safe and persistence and may be used as an effective method for longitudinal follow-up in atrophic gastritis and other precancerous lesions.
authors.affiliation: Department of Gastroenterology, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou, China. sunleimin@yahoo.com
authors.forename: Leimin
authors.initials: L
authors.lastname: Sun
authors.forename: Jianmin
authors.initials: J
authors.lastname: Si
authors.forename: Shujie
authors.initials: S
authors.lastname: Chen
authors.forename: Weili
authors.initials: W
authors.lastname: Liu
authors.forename: Lan
authors.initials: L
authors.lastname: Zhao
authors.forename: Liangjing
authors.initials: L
authors.lastname: Wang
mesh_terms: D000328:Adult; D000368:Aged; D001706:Biopsy / Q000379:methods*; D002244:Carbon; D002908:Chronic Disease; D004396:Coloring Agents; D005260:Female; D005757:Gastritis, Atrophic / Q000473:pathology* / Q000601:surgery; D005773:Gastroscopy; D006801:Humans; D008297:Male; D008875:Middle Aged; D011446:Prospective Studies; D013653:Tattooing / Q000379:methods*
chemical_list: D004396:Coloring Agents; C028433:chinese ink; D002244:Carbon