[Determination of oleuropein in stem of Syringa oblata from different districts by HPLC].
OBJECTIVE
To develop an HPLC method for the determination of oleuropein in Syringa oblata.


METHODS
An Aigilent ZORBAX SB- C18 column (4.6 mm x 250 mm, 5 microm) was used. The mobile phase was acetonitrile-water (21 : 79). The flow rate was 1.0 mL x min(-1). The detection wavelenghth was set at 232 nm and the column temperature was 30 degrees C.


RESULTS
The linear range of oleuropein were from 0.011 62 g x L(-1) to 1.162 g x L(-1). The average recovery was 98.7% with RSD 2.5% (n=9).


CONCLUSIONS
The method is reliable, accurate and specific. It can be used for quality control of the stem of Syringa oblata.
Wei
Xiaohui
X
Shanghai R&D Center for Standardization of Traditional Chinese Medicine, Shanghai 201203, China. xhweixh@163.com
Zhang
Shuxia
S
Zhai
Weifeng
W
Zhang
Shujun
S
Wang
Zhengtao
Z
D000097:Acetonitriles / Q000737:chemistry; D001565:Benzoates / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004365:Drugs, Chinese Herbal; D057888:Iridoid Glucosides; D039823:Iridoids; D011714:Pyrans / Q000032:analysis*; D031662:Syringa / Q000737:chemistry*; D014665:Vasodilator Agents / Q000032:analysis*
D000097:Acetonitriles; D001565:Benzoates; D004365:Drugs, Chinese Herbal; D057888:Iridoid Glucosides; D039823:Iridoids; D011714:Pyrans; D014665:Vasodilator Agents; C002769:oleuropein; C032159:acetonitrile