A tryptamine derivative, SST-VEDI-1, inhibits apoptosis and stimulates mineralization in osteoblasts.
SST-VEDI-1(VEDI-1) is a new synthetic compound that is synthesized from tryptamine, and has structural similarity to the SSH-BM family of compounds. However, the biological effects of VEDI-1 have yet to be well characterized. A recent report has demonstrated that SSH-BM-type compounds can stimulate osteoblast activity in cultured scales of goldfish. In this study, we examined the effects of VEDI-1 on osteoblastic differentiation as well as its effects on apoptosis, which is known to be closely related to osteoblastic differentiation. We found that VEDI-1 enhanced the formation of mineralized nodules in rat osteoblast cell lines, including ROS17/2.8 cells, and in mouse pre-osteoblast cell lines, including MC3T3-E1 cells, in a dose dependent manner, which was accompanied by increased expression of late osteoblast markers, bone sialoprotein (BSP) and osteocalcin (OC). Furthermore, VEDI-I inhibited apoptotic cell death and regulated the expression of proteins in the Bcl-2 family. These results suggest that VEDI-1 may facilitate late differentiation of osteoblasts and may have an inhibitory effect on apoptosis.
Mikami
Yoshikazu
Y
Department of Anatomy, Nihon University School of Dentistry, Tokyo, Japan. mikami-t@dent.nihon-u.ac.jp
Somei
Masanori
M
Takagi
Minoru
M
D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D002113:Calcification, Physiologic / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D058574:Integrin-Binding Sialoprotein; D051379:Mice; D010006:Osteoblasts / Q000187:drug effects*; D015675:Osteocalcin / Q000096:biosynthesis; D010012:Osteogenesis / Q000187:drug effects / Q000235:genetics; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000096:biosynthesis; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D012795:Sialoglycoproteins / Q000096:biosynthesis; D015398:Signal Transduction / Q000187:drug effects; D014363:Tryptamines / Q000494:pharmacology*
C546872:Ibsp protein, mouse; C546873:Ibsp protein, rat; D058574:Integrin-Binding Sialoprotein; D019253:Proto-Oncogene Proteins c-bcl-2; D012333:RNA, Messenger; C542006:SST-VEDI-1 compound; D012795:Sialoglycoproteins; D014363:Tryptamines; D015675:Osteocalcin