title: Cloning and functional analysis of cis-prenyltransferase from Thermobifida fusca.
abstract: cis-Prenyltransferase catalyzes the synthesis of Z,E-mixed prenyl diphosphates by a condensation of isopentenyl diphosphate to an allylic diphosphate. A novel gene encoding a cis-prenyltransferase is cloned from Thermobifida fusca. It showed a unique substrate specificity accepting dimethylallyl diphosphate as a shortest allylic substrate, and synthesizes polyprenyl products up to C(70).
authors.affiliation: Institute of Multidisciplinary Research for Advanced Materials, Tohoku University, Katahira 2-1-1, Aoba-ku, Sendai 980-8577, Japan.
authors.forename: Takanori
authors.initials: T
authors.lastname: Ambo
authors.forename: Motoyoshi
authors.initials: M
authors.lastname: Noike
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Kurokawa
authors.forename: Tanetoshi
authors.initials: T
authors.lastname: Koyama
mesh_terms: D000192:Actinomycetales / Q000201:enzymology*; D001426:Bacterial Proteins / Q000302:isolation & purification / Q000378:metabolism*; D003001:Cloning, Molecular; D013379:Substrate Specificity; D014166:Transferases / Q000302:isolation & purification / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D014166:Transferases; C106821:cis-prenyl transferase