title: [Correlation of lymphoblastic PPAR-gamma mRNA expression with plasma IL-13 contents in children with acute idiopathic thrombocytopenic purpura].
abstract: OBJECTIVE
  PPAR-gamma is associated with the differentiation, apoptosis, proliferation and cytokine secretion of immunologic cells. This study investigated peripheral blood lymphoblastic PPAR-gamma mRNA expression and its correlation with plasma IL-13 contents in children with acute idiopathic thrombocytopenic purpura (ITP).
  
  
  METHODS
  Fifty-three children with acute ITP who were in line with the standard test between September 2007 and July 2008 were enrolled. Fifty healthy children during the same period were used as the control group. PPAR-gamma mRNA expression in peripheral blood lymphocytes were detected by RT-PCR. Plasma IL-13 contents were detected using ELISA.
  
  
  RESULTS
  PPAR-gamma mRNA expression in peripheral blood lymphocytes from acute ITP children were significantly higher than that in the control group (0.78 +/- 0.03 vs 0.52 +/- 0.05; P< 0.05). Plasma IL-13 contents in children with acute ITP were also significantly higher than those in the control group (160.21 +/- 34.26 pg/mL vs 121.42 +/- 12.69 pg/mL; P< 0.05). There was a positive correlation between plasma IL-13 level and lymphoblastic PPAR-gamma mRNA expression in children with ITP (r=0.89, P< 0.05).
  
  
  CONCLUSIONS
  PPAR-gamma mRNA expression in peripheral blood lymphocytes increased and were positively correlated with plasma IL-13 contents in children with acute ITP, suggesting that PPAR-gamma and IL-13 might participate in the pathogenesis of acute ITP.
authors.affiliation: Department of Microbiology and Immunology, Guangdong Medical College, Zhanjiang, Guangdong, China.
authors.forename: Cheng-Qiang
authors.initials: CQ
authors.lastname: Jin
authors.forename: Fang
authors.initials: F
authors.lastname: Liu
authors.forename: Hong
authors.initials: H
authors.lastname: Xiao
authors.forename: Wen-Juan
authors.initials: WJ
authors.lastname: Wang
authors.forename: Qun
authors.initials: Q
authors.lastname: Chen
authors.forename: Bi-Ying
authors.initials: BY
authors.lastname: Zheng
authors.forename: Guo-Ming
authors.initials: GM
authors.lastname: Li
mesh_terms: D000208:Acute Disease; D002648:Child; D002675:Child, Preschool; D005260:Female; D006801:Humans; D018793:Interleukin-13 / Q000097:blood* / Q000502:physiology; D008214:Lymphocytes / Q000378:metabolism*; D008297:Male; D047495:PPAR gamma / Q000235:genetics* / Q000502:physiology; D016553:Purpura, Thrombocytopenic, Idiopathic / Q000209:etiology / Q000276:immunology*; D012333:RNA, Messenger / Q000032:analysis*
chemical_list: D018793:Interleukin-13; D047495:PPAR gamma; D012333:RNA, Messenger