Impact of lifelong sedentary behavior on mitochondrial function of mice skeletal muscle.
This study investigated the impact of lifelong sedentariness on skeletal muscle mass and mitochondrial function. Thirty C57BL/6 strain mice (2 months) were randomly divided into three groups (young-Y; old sedentary-OS; old active-OA). Young animals were sacrificed after 1 week of quarantine, and OS and OA groups were individually placed into standard cages and in cages with running wheels, respectively, until sacrifice (25 months). Body weights and hind-limb skeletal muscle wet weights were obtained from all groups. Mitochondrial respiratory functional measures (i.e., state 3 and 4 respiration, respiratory control ratio, and ratio of nanomoles of ADP phosphorylated by nanomoles of O2 consumed [ADP/O]) and biochemical markers of oxidative damage (aconitase activity, protein carbonyl derivatives, sulfhydryl groups) were measured in isolated mitochondrial suspensions. Our results reveal that lifelong sedentary behavior has a negative impact on the age-related loss of skeletal muscle mass and on the isolated mitochondrial function of mixed skeletal muscle of mice, which is associated with an increased oxidative damage to mitochondrial biomolecules.
Figueiredo
Pedro A
PA
Centro de Investigação em Actividade Física, Saúde e Lazer, Faculty of Sports, University of Porto, Rua Dr. Plácido Costa, 91, 4200-450 Porto, Portugal. pfigueiredo@ismai.pt
Powers
Scott K
SK
Ferreira
Rita M
RM
Amado
Francisco
F
Appell
Hans J
HJ
Duarte
José A
JA
D000154:Aconitate Hydratase / Q000378:metabolism; D000244:Adenosine Diphosphate / Q000494:pharmacology; D000375:Aging / Q000502:physiology*; D000818:Animals; D002950:Citrate (si)-Synthase / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D008931:Mitochondria, Muscle / Q000502:physiology*; D018482:Muscle, Skeletal / Q000502:physiology*; D018384:Oxidative Stress; D010101:Oxygen Consumption; D010766:Phosphorylation; D010805:Physical Conditioning, Animal
D000244:Adenosine Diphosphate; D002950:Citrate (si)-Synthase; D000154:Aconitate Hydratase