title: Assessment of feasibility of maillard reaction between baclofen and lactose by liquid chromatography and tandem mass spectrometry, application to pre formulation studies.
abstract: The aim of this study was to determine any possible, baclofen-lactose Maillard reaction products. Granules and tablets of baclofen and lactose were prepared and maintained in heat ovens for a certain time period. The effects of lactose type, addition of magnesium stearate, and water were monitored. Heated lactose and baclofen were analyzed using reverse-phase HPLC. Liquid chromatography tandem mass spectroscopy revealed nominal mass values consistent with baclofen-lactose, early-stage Maillard reaction condensation products (ESMRP). Multiple reaction monitoring confirmed the presence of ESMRP as well. FTIR analysis proved the formation of imine bond. The results indicated that baclofen undergoes a Maillard-type reaction with lactose.
authors.affiliation: Department of Pharmaceutics, Tabriz University of Medical Sciences, Tabriz, Iran.
authors.forename: Farnaz
authors.initials: F
authors.lastname: Monajjemzadeh
authors.forename: Davoud
authors.initials: D
authors.lastname: Hassanzadeh
authors.forename: Hadi
authors.initials: H
authors.lastname: Valizadeh
authors.forename: Mohammad R
authors.initials: MR
authors.lastname: Siahi-Shadbad
authors.forename: Javid Shahbazi
authors.initials: JS
authors.lastname: Mojarrad
authors.forename: Thomas
authors.initials: T
authors.lastname: Robertson
authors.forename: Michael S
authors.initials: MS
authors.lastname: Roberts
mesh_terms: D001418:Baclofen / Q000737:chemistry*; D002626:Chemistry, Pharmaceutical; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D007785:Lactose / Q000737:chemistry*; D015416:Maillard Reaction; D017550:Spectroscopy, Fourier Transform Infrared; D013607:Tablets / Q000737:chemistry; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D013607:Tablets; D001418:Baclofen; D007785:Lactose