Optimization of fermentation conditions for production of anti-TMV extracellular ribonuclease by Bacillus cereus using response surface methodology.
Bacillus cereus ZH14 was previously found to produce a new type of antiviral ribonuclease, which was secreted into medium and active against tobacco mosaic virus. In order to enhance the ribonuclease production, in this study the optimization of culture conditions using response surface methodology was done. The fermentation variables including culture temperature, initial pH, inoculum size, sucrose, yeast extract, MgSO(4).7H(2)O, and KNO(3) were considered for selection of significant ones by using the Plackett-Burman design, and four significant variables (sucrose, yeast extract, MgSO(4).7H(2)O, and KNO(3)) were further optimized by a 2(4) factorial central composite design. The optimal combination of the medium constituents for maximum ribonuclease production was determined as 8.50 g/l sucrose, 9.30 g/l yeast extract, 2.00 g/l MgSO(4).7H(2)O, and 0.62 g/l KNO(3). The enzyme activity was increased by 60%. This study will be helpful to the future commercial development of the new bacteria-based antiviral ribonuclease fermentation process.
Zhou
Wen-Wen
WW
Key Laboratory of Microbial Metabolism, Ministry of Education, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, 800 Dong-Chuan Road, Shanghai, 200240, China. zww2003@gmail.com
He
Yun-Long
YL
Niu
Tian-Gui
TG
Zhong
Jian-Jiang
JJ
D001409:Bacillus cereus / Q000201:enzymology* / Q000254:growth & development; D001426:Bacterial Proteins / Q000096:biosynthesis*; D018929:Cell Culture Techniques / Q000379:methods*; D003470:Culture Media; D005285:Fermentation; D015999:Multivariate Analysis; D012044:Regression Analysis; D012260:Ribonucleases / Q000096:biosynthesis* / Q000302:isolation & purification; D014027:Tobacco Mosaic Virus
D001426:Bacterial Proteins; D003470:Culture Media; D012260:Ribonucleases