[Effects of culture conditions on biomass and active components of suspension cells of Panax quinquefolium].
OBJECTIVE
To study the effects of inoculum, various media, pH value of medium and illumination conditions on the growth of Panax quinquefolium suspension cells and the synthesis of ginsenosides Re, Rb1 and polysaccharides.


METHODS
The suspension cells were obtained through tissue culture by manipulation of inoculum, various media, pH value, and illumination conditions. The contents of ginsenosides Re and Rb1 were determined by HPLC, while the contents of polysaccharide were determined by ultraviolet spectrophotometry.


RESULTS
The growth rate of suspension cells was greatly increased when inoculum amount was 25 g x L(-1). The effect of media MS, SH and B5 on suspension cells was observed. MS medium was favorable for cells growth, while B5 medium was favorable for the synthesis of ginsenosides and polysaccharides. The polysaccharide content in three media were higher than that of the cultivations. The pH value showed little influence on the cells growth, medium pH 6.0 enhanced the synthesis of Re and polysaccharides. Illumination could significantly enhance secondary metabolite biosynthesis of suspension cells and promoted slightly in polysaccharide synthesis.


CONCLUSIONS
The inoculum, various media, pH value of medium and illumination conditions have significant influences on suspension cells growth of P. quinquefolium, secondary metabolite and polysaccharides synthesis.
Wang
Juan
J
Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China.
Gao
Wenyuan
W
Huang
Tao
T
Cao
Yu
Y
D018533:Biomass; D018929:Cell Culture Techniques; D019411:Clinical Laboratory Techniques; D003470:Culture Media; D036145:Ginsenosides / Q000378:metabolism; D005894:Panax / Q000737:chemistry* / Q000254:growth & development / Q000378:metabolism; D011134:Polysaccharides / Q000378:metabolism; D013535:Suspensions / Q000032:analysis / Q000737:chemistry*
D003470:Culture Media; D036145:Ginsenosides; D011134:Polysaccharides; D013535:Suspensions; C049864:ginsenoside Re