title: Design and fabrication of a polyimide-based microelectrode array: application in neural recording and repeatable electrolytic lesion in rat brain.
abstract: The design and testing of a new microelectrode array, the NCTU (National Chiao Tung University) probe, was presented. Evaluation results showed it has good biocompatibility, high signal-to-noise ratio (SNR: the root mean square of background noise to the average peak-to-peak amplitude of spikes) during chronic neural recordings, and high reusability for electrolytic lesions. The probe was a flexible, polyimide-based microelectrode array with a long shaft (14.9 mm in length) and 16 electrodes (5 microm-thick and 16 microm in radius); its performance in chronic in vivo recordings was examined in rodents. To improve the precision of implantation, a metallic, impact-resistant layer was sandwiched between the polyimide layers to strengthen the probe. The three-dimensional (3D) structure of electrodes fabricated by electroplating produced rough textures that increased the effective surface area. The in vitro impedance of electrodes on the NCTU probe was 2.4+/-0.52 MOmega at 1 kHz. In addition, post-surgical neural recordings of implanted NCTU probes were conducted for up to 40 days in awake, normally behaving rats. The electrodes on the NCTU probe functioned well and had a high SNR (range: 4-5) with reliable in vivo impedance (<0.7 MOmega). The electrodes were also robust enough to functionally record events, even after the anodal current (30 microA, 10s) was repeatedly applied for 60 times. With good biocompatibility, high and stable SNR for chronic recording, and high tolerance for electrolytic lesion, the NCTU probe would serve as a useful device in future neuroscience research.
authors.affiliation: Department of Electrical and Control Engineering, National Chiao Tung University, No. 1001 Ta-Hsueh Road, Hsinchu 300, Taiwan, ROC. irradiance@so-net.net.tw
authors.forename: You-Yin
authors.initials: YY
authors.lastname: Chen
authors.forename: Hsin-Yi
authors.initials: HY
authors.lastname: Lai
authors.forename: Sheng-Huang
authors.initials: SH
authors.lastname: Lin
authors.forename: Chien-Wen
authors.initials: CW
authors.lastname: Cho
authors.forename: Wen-Hung
authors.initials: WH
authors.lastname: Chao
authors.forename: Chia-Hsin
authors.initials: CH
authors.lastname: Liao
authors.forename: Siny
authors.initials: S
authors.lastname: Tsang
authors.forename: Yi-Fan
authors.initials: YF
authors.lastname: Chen
authors.forename: Si-Yue
authors.initials: SY
authors.lastname: Lin
mesh_terms: D000818:Animals; D001921:Brain / Q000502:physiology*; D004567:Electrodes, Implanted; D004569:Electroencephalography / Q000295:instrumentation*; D004572:Electrolysis; D004867:Equipment Design; D019544:Equipment Failure Analysis; D008297:Male; D008839:Microelectrodes; D051381:Rats; D017208:Rats, Wistar; D012117:Resins, Synthetic / Q000737:chemistry*; D012680:Sensitivity and Specificity
chemical_list: D012117:Resins, Synthetic; C029945:polyimide resin