Antioxidative role of selenoprotein W in oxidant-induced mouse embryonic neuronal cell death.
It has been reported that selenoprotein W (SelW) mRNA is highly expressed in the developing central nerve system of rats, and its expression is maintained until the early postnatal stage. We here found that SelW protein significantly increased in mouse brains of postnatal day 8 and 20 relative to embryonic day 15. This was accompanied by increased expression of SOD1 and SOD2. When the expression of SelW in primary cultured cells derived from embryonic cerebral cortex was knocked down with small interfering RNAs (siRNAs), SelW siRNA-transfected neuronal cells were more sensitive to the oxidative stress induced by treatment of H2O2 than control cells. TUNEL assays revealed that H2O2-induced apoptotic cell death occurred at a higher frequency in the siRNA-transfected cells than in the control cells. Taken together, our findings suggest that SelW plays an important role in protection of neurons from oxidative stress during neuronal development.
Chung
Youn Wook
YW
Laboratory of Cellular and Molecular Biochemistry, School of Life Sciences and Biotechnology, Korea University, Seoul 136-701, Korea.
Jeong
Daewon
D
Noh
Ok Jeong
OJ
Park
Yong Hwan
YH
Kang
Soo Im
SI
Lee
Min Goo
MG
Lee
Tae-Hoon
TH
Yim
Moon Bin
MB
Kim
Ick Young
IY
D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D017209:Apoptosis / Q000235:genetics; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000473:pathology; D004622:Embryo, Mammalian; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D006861:Hydrogen Peroxide / Q000378:metabolism; D051379:Mice; D009420:Nervous System / Q000196:embryology* / Q000254:growth & development / Q000473:pathology*; D009474:Neurons / Q000378:metabolism* / Q000473:pathology; D018384:Oxidative Stress; D034741:RNA, Small Interfering / Q000235:genetics; D051151:Selenoprotein W / Q000235:genetics / Q000378:metabolism*; D013482:Superoxide Dismutase / Q000235:genetics / Q000378:metabolism; D000072105:Superoxide Dismutase-1; D000099156:Superoxide Dismutase 2
D000975:Antioxidants; D006861:Hydrogen Peroxide; D034741:RNA, Small Interfering; D051151:Selenoprotein W; D013482:Superoxide Dismutase; D000072105:Superoxide Dismutase-1; D000099156:Superoxide Dismutase 2; C000606291:Sod1 protein, mouse; C000606292:Sod1 protein, rat