title: Rapid detection of the O25b-ST131 clone of Escherichia coli encompassing the CTX-M-15-producing strains.
abstract: OBJECTIVE
  Recently, a CTX-M-15 extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli O25b-ST131 clone, belonging to the B2 phylogenetic group and with a high virulence potential, has been reported all over the world, representing a major public health problem. The present study was carried out to develop a rapid and simple detection assay that identifies members of this clone.
  
  
  METHODS
  A total of 627 E. coli isolates of which 373 produced an ESBL, collected across four continents, were screened using a O25b-ST131 clone allele-specific PCR for the pabB gene.
  
  
  RESULTS
  One hundred and forty-three ESBL isolates were found positive with the assay. These isolates were all of O25b type and, when studied by multilocus sequence typing (25 cases), were all of ST131. The O25b-ST131 clone was found to produce ESBLs other than CTX-M-15, specifically CTX-M-2, -3, -14, -27, -32 and -61 as well as TEM-24. This clone represents 3% of non-ESBL B2 isolates originating from urinary tract infections in Paris.
  
  
  CONCLUSIONS
  We have developed a PCR-based assay that easily identifies a clone with high likelihood of producing ESBLs, including CTX-M-15.
authors.affiliation: INSERM U722 and Université Paris 7, Faculté de Médecine, Site Xavier Bichat, 75018 Paris, France.
authors.forename: Olivier
authors.initials: O
authors.lastname: Clermont
authors.forename: Hiran
authors.initials: H
authors.lastname: Dhanji
authors.forename: Mathew
authors.initials: M
authors.lastname: Upton
authors.forename: Tarek
authors.initials: T
authors.lastname: Gibreel
authors.forename: Andrew
authors.initials: A
authors.lastname: Fox
authors.forename: David
authors.initials: D
authors.lastname: Boyd
authors.forename: Michael R
authors.initials: MR
authors.lastname: Mulvey
authors.forename: Patrice
authors.initials: P
authors.lastname: Nordmann
authors.forename: Etienne
authors.initials: E
authors.lastname: Ruppé
authors.forename: Jean Louis
authors.initials: JL
authors.lastname: Sarthou
authors.forename: Thierry
authors.initials: T
authors.lastname: Frank
authors.forename: Sophie
authors.initials: S
authors.lastname: Vimont
authors.forename: Guillaume
authors.initials: G
authors.lastname: Arlet
authors.forename: Catherine
authors.initials: C
authors.lastname: Branger
authors.forename: Neil
authors.initials: N
authors.lastname: Woodford
authors.forename: Erick
authors.initials: E
authors.lastname: Denamur
mesh_terms: D004926:Escherichia coli / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D004927:Escherichia coli Infections / Q000175:diagnosis* / Q000382:microbiology*; D029968:Escherichia coli Proteins / Q000235:genetics; D006801:Humans; D010297:Paris; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D001618:beta-Lactamases / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D029968:Escherichia coli Proteins; C473109:beta-lactamase CTX-M-15; D001618:beta-Lactamases