[Detection of antimalarial activity for new compounds by isotopic microtest].
Two clone lines (Dd2 and 3D7) of Plasmodium falciparum were cultivated continuously in human erythrocytes at 37 degrees C in RPMI 1640 medium with human serum and subjected to 6% sorbitol treatment 2 times in order to obtain highly synchronized cultures. The second generation parasites after the treatment were diluted with human RBC to be a suspension of P. falciparum-human RBC at 2.5% hematocrit and 0.5% parasitemia, and 2 microCi/ml of 8-3H-hypoxanthine was added. Isotopic microtest was employed to detect the antimalarial activity for 20 new compounds. Results revealed that the 20 compounds showed no anti-malarial activity, while the control drugs, chloroquine and quinine, exhibited high efficacy, indicating that the isotopic microtest is a stable and reproducible assay for screening new antimalarials.
Chen
Zhao-guo
ZG
Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Key Laboratory of Animal Parasitology, Ministry of Agriculture, Shanghai 200232, China. zhaoguochen70@yahoo.com.cn
Moreno
Alicia
A
Benito
Agustin
A
Moreno
Marta
M
Berzosa
Pedro J
PJ
de Lucio
Aida
A
Moyano
Eva
E
D000962:Antimalarials / Q000032:analysis* / Q000494:pharmacology*; D004353:Drug Evaluation, Preclinical; D004912:Erythrocytes / Q000469:parasitology; D006801:Humans; D021261:Parasitic Sensitivity Tests; D010963:Plasmodium falciparum / Q000187:drug effects*
D000962:Antimalarials