title: Porcine bone marrow stromal cell differentiation on heparin-adsorbed poly(e-caprolactone)-tricalcium phosphate-collagen scaffolds.
abstract: We evaluate the potential of heparin as a substrate component for the fabrication of bone tissue engineering constructs using poly(e-caprolactone)-tricalcium phosphate-collagen type I (PCL-TCP-Col) three-dimensional (3-D) scaffolds. First we explored the ability of porcine bone marrow precursor cells (MPCs) to differentiate down both the adipogenic and osteogenic pathways within 2-D culture systems, with positive results confirmed by Oil-Red-O and Alizarin Red staining, respectively. Secondly, we examined the influence of heparin on the interaction and behaviour of MPCs when seeded onto PCL-TCP-Col 3-D scaffolds, followed by their induction into the osteogenic lineage. Our 3-D findings suggest that cell metabolism and proliferation increased between days 1 and 14, with deposition of extracellular matrix also observed up to 28 days. However, no noticeable difference could be detected in the extent of osteogenesis for PCL-TCP-Col scaffolds groups with the addition of heparin compared to identical control scaffolds without the addition of heparin.
authors.affiliation: Division of Bioengineering, Faculty of Engineering, National University of Singapore, Singapore 119260, Singapore.
authors.forename: Zhi Zhen
authors.initials: ZZ
authors.lastname: Chum
authors.forename: Maria A
authors.initials: MA
authors.lastname: Woodruff
authors.forename: Simon M
authors.initials: SM
authors.lastname: Cool
authors.forename: Dietmar W
authors.initials: DW
authors.lastname: Hutmacher
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry; D001854:Bone Marrow Cells / Q000166:cytology / Q000502:physiology*; D002130:Calcium Phosphates / Q000737:chemistry*; D002208:Caproates / Q000737:chemistry*; D018929:Cell Culture Techniques / Q000379:methods; D002454:Cell Differentiation / Q000502:physiology*; D002478:Cells, Cultured; D003094:Collagen / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry; D006493:Heparin / Q000737:chemistry*; D007783:Lactones / Q000737:chemistry*; D008422:Materials Testing; D011108:Polymers / Q000737:chemistry; D017154:Stromal Cells / Q000166:cytology / Q000502:physiology*; D034421:Sus scrofa; D023822:Tissue Engineering / Q000379:methods; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D001672:Biocompatible Materials; D002130:Calcium Phosphates; D002208:Caproates; D005456:Fluorescent Dyes; D007783:Lactones; D011108:Polymers; C121056:caprolactone; D006493:Heparin; D003094:Collagen; C018392:tricalcium phosphate