Development of a cytochrome c-based screen-printed biosensor for the determination of the antioxidant capacity of orange juices.
This paper describes the development of an amperometric cytochrome c (cyt c)-based biosensor and its later application to the quantification of the scavenging capacity of antioxidants. The enzymatic biosensor was constructed by covalently co-immobilizing both cyt c and XOD on a mercaptoundecanol/mercaptoundecanoic acid (MU/MUA) mixed self-assembled monolayer (SAM)-modified screen-printed gold electrode. The applicability of this method was shown by analyzing the antioxidant capacity of pure substances, such as ascorbic acid and Trolox, and natural sources of antioxidants, particularly 5 orange juices.
Cortina-Puig
Montserrat
M
IMAGES EA 4218, Université de Perpignan Via Domitia, 52 Avenue Paul Alduy, 66860 Perpignan Cedex, France.
Muñoz-Berbel
Xavier
X
Rouillon
Regis
R
Calas-Blanchard
Carole
C
Marty
Jean-Louis
JL
D000818:Animals; D000975:Antioxidants / Q000032:analysis / Q000737:chemistry* / Q000378:metabolism; D001628:Beverages; D015374:Biosensing Techniques / Q000379:methods*; D002417:Cattle; D032084:Citrus sinensis / Q000737:chemistry*; D045304:Cytochromes c / Q000737:chemistry* / Q000378:metabolism; D004563:Electrochemistry; D004566:Electrodes; D004800:Enzymes, Immobilized / Q000737:chemistry / Q000378:metabolism; D006046:Gold / Q000737:chemistry / Q000378:metabolism; D006736:Horses; D011327:Printing; D013481:Superoxides / Q000737:chemistry / Q000378:metabolism; D014969:Xanthine Oxidase / Q000737:chemistry / Q000378:metabolism
D000975:Antioxidants; D004800:Enzymes, Immobilized; D013481:Superoxides; D006046:Gold; D045304:Cytochromes c; D014969:Xanthine Oxidase