Caffeic acid phenethyl ester prevents ovary ischemia/reperfusion injury in rabbits.
Protective effect of caffeic acid phenethyl ester (CAPE) on ovary ischemia/reperfusion (IR) injury was investigated in this study. Twenty four New Zealand rabbits were divided into 4 groups as follows: group S served as sham. Group C was intraperitoneally injected with CAPE (8.5mg/kg). In groups E+IR and C+IR, 1% ethanol and CAPE was given intraperitoneally before torsion, respectively. Then, the ovaries were subjected to IR in both groups. Ovary reduced glutathione (GSH) level and glutathione peroxidase (GSH-Px) activity in group E+IR were significantly reduced compared to that of group S. GSH level and GSH-Px activity was significantly increased in group C+I/R. Thiobarbituric acid reactive substances (TBARS) and catalase (CAT) activity in group E+I/R was significantly higher than in group S. CAT activity was decreased to normal levels by CAPE treatment in group C+I/R, while TBARS in group C+IR was significantly reduced compared to that of E+IR. According to histopathological examination, severe congestion, hemorrhage, edema and leukocyte infiltration were observed in E+I/R group. CAPE prominently reduced degenerative effects of IR injury thus it alleviates free radical damage. In conclusion, CAPE which is able to prevent IR-induced injury in the ovaries may be of therapeutic value before the surgical correction.
Kart
A
A
Department of Pharmacology and Toxicology, College of Veterinary Medicine, University of Kafkas, 36100 Kars, Turkey. asimkart@hotmail.com
Cigremis
Y
Y
Ozen
H
H
Dogan
O
O
D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D002109:Caffeic Acids / Q000633:toxicity*; D002374:Catalase / Q000378:metabolism; D005260:Female; D005978:Glutathione / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D010049:Ovarian Diseases / Q000517:prevention & control*; D010626:Phenylethyl Alcohol / Q000031:analogs & derivatives* / Q000633:toxicity; D011817:Rabbits; D015427:Reperfusion Injury / Q000517:prevention & control*; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
D000975:Antioxidants; D002109:Caffeic Acids; D017392:Thiobarbituric Acid Reactive Substances; D002374:Catalase; C055494:caffeic acid phenethyl ester; D005978:Glutathione; D010626:Phenylethyl Alcohol