Macrophage migration inhibitory factor homologs of anisakis simplex suppress Th2 response in allergic airway inflammation model via CD4+CD25+Foxp3+ T cell recruitment.
We have cloned the macrophage migration inhibitory factor (MIF)-like protein (Anisakis simplex (As)-MIF) from larvae of the whale worm (Anisakis simplex third-stage larvae). Asthma was induced in the mice using OVA/alum, with or without various concentrations of rAs-MIF treatment before OVA/alum challenge. Treatment with rAs-MIF coupled with OVA/alum during the challenge period induced a complete inhibition of eosinophilia and goblet cell hyperplasia within the lung and profoundly ameliorated the development of lung hyperreactivity. Also, rAs-MIF was shown to reduce profoundly the quantity of Th2-related cytokines (IL-4, IL-5, and IL-13) in the bronchial alveolar lavage fluid and allergen-specific IgG2a in sera. IL-10 and TGF-beta levels in the bronchoalveolar lavage fluid of the rAs-MIF-treated group were significantly higher than in the other groups. Additionally, CD4(+)CD25(+)Foxp3(+) T cells (regulatory T) were recruited to the spleen and lungs of the rAs-MIF-treated mice, but this recruitment was inhibited by anti-rAs-MIF Ab.
Park
Sang Kyun
SK
Department of Parasitology, School of Medicine, Pusan National University Busan, Busan, Korea.
Cho
Min Kyoung
MK
Park
Hye-Kyung
HK
Lee
Keun Hee
KH
Lee
Sun Joo
SJ
Choi
Seon Hee
SH
Ock
Mee Sun
MS
Jeong
Hae Jin
HJ
Lee
Moo Hyung
MH
Yu
Hak Sun
HS
D000818:Animals; D017163:Anisakis; D001249:Asthma / Q000276:immunology*; D001992:Bronchoalveolar Lavage Fluid / Q000276:immunology; D002634:Chemotaxis, Leukocyte / Q000276:immunology*; D016207:Cytokines / Q000032:analysis; D004195:Disease Models, Animal; D007249:Inflammation / Q000276:immunology; D008168:Lung / Q000276:immunology; D008263:Macrophage Migration-Inhibitory Factors / Q000276:immunology*; D051379:Mice; D013154:Spleen / Q000276:immunology; D050378:T-Lymphocytes, Regulatory / Q000276:immunology*; D018418:Th2 Cells / Q000276:immunology*
D016207:Cytokines; D008263:Macrophage Migration-Inhibitory Factors