Microarray analysis of microRNA expression in renal clear cell carcinoma.
OBJECTIVE
To explore the microRNA (miRNA) expression in renal clear cell carcinoma (RCCC).


METHODS
We compared the miRNA expression profiles in 11 pairs of RCCC and adjacent nontumorous tissue (NT) from 11 RCCC patients, using a mammalian miRNA microarray containing whole human mature and precursor miRNA sequences. To verify microarray results, Northern blotting was carried out on 5 randomly selected miRNAs.


RESULTS
Totally 81 miRNAs were identified valid expression in RCCC samples, 48 of which specifically detected in RCCC samples, 17 of which detected downregulated in RCCC compared to NT sample, 2 upregulated and 14 without significant difference. MiRNAs in RCCC tissues exhibit an overall higher expression level than NT tissue. The chip results were confirmed by northern blot analysis.


CONCLUSIONS
Our study may help to clarify the molecular mechanisms involved in the pathogenesis of RCCC, and miRNAs potentially serve as a novel diagnostic biomarker of RCCC.
Huang
Y
Y
Key Laboratory of Laboratory Medical Diagnostics, Ministry of Education, Chongqing Medical University, Chongqing 400016, China.
Dai
Y
Y
Yang
J
J
Chen
T
T
Yin
Y
Y
Tang
M
M
Hu
C
C
Zhang
L
L
D000328:Adult; D014408:Biomarkers, Tumor / Q000235:genetics* / Q000378:metabolism; D002292:Carcinoma, Renal Cell / Q000235:genetics* / Q000503:physiopathology; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007680:Kidney Neoplasms / Q000235:genetics* / Q000503:physiopathology; D008297:Male; D035683:MicroRNAs / Q000378:metabolism*; D046228:Microarray Analysis; D008875:Middle Aged
D014408:Biomarkers, Tumor; D035683:MicroRNAs; C541010:MIRN145 microRNA, human; C524941:MIRN17 microRNA, human; C525709:MIR221, human