Comparison between L and E gene amplification analytical methods for human papillomavirus typing.
OBJECTIVE
L and E6/E7 gene amplification analyses were compared to identify human papillomavirus (HPV) infection and verify the HPV type, with the intent to minimize HPV typing errors.


METHODS
L1 gene verified HPV typing was accomplished via polymerase chain reaction (PCR) and membrane assays. Verification of HPV typing via E6/E7 genes was accomplished through nested multiplexed PCR. The results from 104 samples were compared.


RESULTS
The rates of accordance and difference were 35% and 65%, respectively. For 29% of the analyses, nested multiplexed PCR was more diversified than the membrane assay.


CONCLUSIONS
HPV can be classified into low-risk HPV and high-risk HPV groups. In parallel amplifications of the L and E genes is more efficient for accurate diagnosis in light of the different symptoms and attendant precautions of the risk groups.
Cho
Hong-Bum
HB
Department of Biological Engineering, SeoKyeong University, Seoul, Korea.
Kim
Young-Jae
YJ
Kim
Kyung-Tai
KT