Effect of beraprost sodium (BPS) in a new rat partial unilateral ureteral obstruction model.
Unilateral ureteral obstruction (UUO) is a representative model for investigating the common mechanism of decreasing renal function in chronic renal failure. In this study, we present a new partial UUO model in adult rats and evaluated the effect of beraprost sodium (BPS: stable prostaglandin I(2) (PGI(2)) analog). We could make reproductive and uniform partial UUO by ligating the left ureter together with a 0.5 mm diameter stainless steel wire with nylon thread, and withdrawing the stainless wire. One week later, the ureteral obstruction was released. After 3 weeks from the release of UUO, all animals of control group, without BPS administration, developed basophilic degeneration of tubular epithelium, tubular dilatation and interstitial fibrosis. The areas of tubular degeneration and fibrosis were significantly reduced in the BPS group, orally administered BPS 300 microg/kg twice a day from the next day of the release of obstruction, than in control group. In conclusion, we can established the adult rat partial UUO-release model and revealed that BPS can inhibit renal tubular damage and tubulointerstitial fibrosis.
Takenaka
Masahiko
M
Takenaka Animal Hospital, 3-10-3 Zao-cho, Fukuyama, Hiroshima 721-0971, Japan.
Machida
Noboru
N
Ida
Nobutaka
N
Satoh
Nahoko
N
Kurumatani
Hajimu
H
Yamane
Yoshihisa
Y
D000818:Animals; D004195:Disease Models, Animal; D011464:Epoprostenol / Q000031:analogs & derivatives* / Q000494:pharmacology / Q000627:therapeutic use; D005355:Fibrosis; D007676:Kidney Failure, Chronic / Q000188:drug therapy* / Q000209:etiology / Q000473:pathology; D007684:Kidney Tubules / Q000187:drug effects* / Q000473:pathology; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D014517:Ureteral Obstruction / Q000150:complications* / Q000473:pathology
C048081:beraprost; D011464:Epoprostenol