title: Caspase inhibitors increase the rate of recovery of neural stem/progenitor cells from post-mortem rat brains stored at room temperature.
abstract: To match the demand of regenerative medicine for nerve system, collection of stem cells from the post-mortem body is one of the most practical ways. In this study, the storage condition of the post-mortem body was examined. We prepared neural stem/progenitor cells (NSPCs) from post-mortem rat brains stored at different temperatures. When brains were stored at 4 degrees C, for one week, we were able to obtain neurospheres (a spheroid body containing NSPCs) by stimulation of cells with epidermal growth factor (EGF). Incremental increases in storage temperature decreased the rate of appearance of neurospheres. Within 48 h at 15 degrees C, 24 h at 25 degrees C, in both condition, we were able to recover NSPCs from post-mortem rat brains. At 15 degrees C, 90% of neurosphere-forming activity was lost within 24 h. However, even after 24 h at 25 degrees C, 2% neurosphere-forming activity remained. After 6 h of death, there was very little difference between the rates of NSPC recovery at 4 degrees C and 25 degrees C. Addition of caspase inhibitors to both the rat brain storage solution and the NSPC culture medium increased the rate of neurosphere-forming activity. In particular, an inhibitor of caspase-8 activity increased the NSPC recovery rate approximately three-fold, with no accompanying detrimental effects on neural differentiation in vitro.
authors.affiliation: Department of Tissue Engineering and Cell Therapy, Institute of Biomedical Research and Innovation, 1-5-4 Minatojima-Minamimachi, Chuo-ku, Kobe, Hyogo 650-0047, Japan.
authors.forename: Atsuko
authors.initials: A
authors.lastname: Hasegawa
authors.forename: Chikako
authors.initials: C
authors.lastname: Yamada
authors.forename: Miho
authors.initials: M
authors.lastname: Tani
authors.forename: Shun-ichiro
authors.initials: S
authors.lastname: Hirano
authors.forename: Yasuhito
authors.initials: Y
authors.lastname: Tokumoto
authors.forename: Jun
authors.initials: J
authors.lastname: Miyake
mesh_terms: D000818:Animals; D001921:Brain / Q000166:cytology*; D061945:Caspase Inhibitors; D002454:Cell Differentiation / Q000502:physiology; D002478:Cells, Cultured; D004815:Epidermal Growth Factor / Q000494:pharmacology; D009474:Neurons / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D018874:Spheroids, Cellular / Q000166:cytology / Q000502:physiology; D013234:Stem Cells / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D013696:Temperature
chemical_list: D061945:Caspase Inhibitors; D004815:Epidermal Growth Factor