title: Down-regulation of FcepsilonRI expression by Houttuynia cordata Thunb extract in human basophilic KU812F cells.
abstract: Human basophilic KU812F cells express a high-affinity immunoglobulin (Ig) E receptor, FcepsilonRI, which plays an important role in IgE-mediated allergic reactions. Houttuynia cordata Thunb (Family Saururaceae), which is rich in polyphenols, has been shown to have various physiological properties, including antiviral, antioxidative, anticancer, and anti-inflammatory activities. The effect of H. cordata extract on the expression of FcepsilonRI in human KU812F cells was examined. Flow cytometric analysis showed that the FcepsilonRI expression and the IgE binding activity were suppressed when the cells were cultured with H. cordata extract. Reverse transcription-polymerase chain reaction analysis showed that levels of the mRNAs for FcepsilonRI alpha- and gamma-chains were decreased by the treatment of H. cordata extract. Addition of H. cordata extract to culture medium was also observed to result in a reduction in the release of histamine from the cells. These results suggest that H. cordata extract may exert its anti-allergic activity through down-regulation of FcepsilonRI expression and a subsequent decrease in histamine release.
authors.affiliation: Institute of Marine Life Science, Pukyong National University, Busan, Republic of Korea.
authors.forename: Sun-Yup
authors.initials: SY
authors.lastname: Shim
authors.forename: Young-Kook
authors.initials: YK
authors.lastname: Seo
authors.forename: Jeong-Ro
authors.initials: JR
authors.lastname: Park
mesh_terms: D018926:Anti-Allergic Agents / Q000494:pharmacology*; D001491:Basophils / Q000187:drug effects*; D002460:Cell Line; D015536:Down-Regulation; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005419:Flavonoids / Q000494:pharmacology; D005434:Flow Cytometry; D006632:Histamine / Q000378:metabolism; D006633:Histamine Antagonists / Q000494:pharmacology*; D052859:Houttuynia; D006801:Humans; D007073:Immunoglobulin E / Q000378:metabolism*; D010636:Phenols / Q000494:pharmacology; D059808:Polyphenols; D012333:RNA, Messenger / Q000378:metabolism; D017455:Receptors, IgE / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D018926:Anti-Allergic Agents; D004365:Drugs, Chinese Herbal; C543355:FcepsilonRI alpha-chain, human; C543372:FcepsilonRI gamma-chain, human; D005419:Flavonoids; D006633:Histamine Antagonists; C442781:Houttuynia cordata extract; D010636:Phenols; D059808:Polyphenols; D012333:RNA, Messenger; D017455:Receptors, IgE; D007073:Immunoglobulin E; D006632:Histamine