title: Escherichia coli Nissle 1917 for probiotic use in piglets: evidence for intestinal colonization.
abstract: OBJECTIVE
  This study was prompted to investigate the intestinal localization and colonization of orally administered Escherichia coli Nissle 1917 (EcN) in piglets.
  
  
  RESULTS
  EcN was fed to ten EcN-negative piglets (3 months) over seven consecutive days. Faecal samples were collected repeatedly and tested for EcN-DNA by a combined culture/PCR assay and for viable EcN by culture methods, respectively. EcN-DNA was detectable in faeces of all piglets within the first 24 h after it was added to the feed. After the administration of EcN had been stopped, the presence of EcN-DNA in faecal samples indicated that all piglets shedded EcN with their faeces intermittently through up to 33 days. In addition, E. coli strains indistinguishable from EcN by all markers tested (rdar colony morphotype, multiplex PCR and GEI II-PCR analyses, XbaI-pattern, K5 phage susceptibility) were isolated from faecal samples and from mucosal swabs taken at euthanasia at the end of the experiment.
  
  
  CONCLUSIONS
  EcN colonizes the intestine and persists in conventionally reared piglets for at least 4 weeks upon oral administration.
  
  
  CONCLUSIONS
  Results of this study have implications for efficacy and safety assessments of EcN as a probiotic strain for use in pigs.
authors.affiliation: Institute for Hygiene and Infectious Diseases of Animals, Justus Liebig University Giessen, Giessen, Germany. Stefanie.Barth@vetmed.uni-giessen.de
authors.forename: S
authors.initials: S
authors.lastname: Barth
authors.forename: S
authors.initials: S
authors.lastname: Duncker
authors.forename: J
authors.initials: J
authors.lastname: Hempe
authors.forename: G
authors.initials: G
authors.lastname: Breves
authors.forename: G
authors.initials: G
authors.lastname: Baljer
authors.forename: R
authors.initials: R
authors.lastname: Bauerfeind
mesh_terms: D000284:Administration, Oral; D000818:Animals; D004269:DNA, Bacterial / Q000032:analysis; D004926:Escherichia coli / Q000235:genetics / Q000302:isolation & purification*; D005243:Feces / Q000382:microbiology; D007422:Intestines / Q000382:microbiology*; D057230:Limit of Detection; D016133:Polymerase Chain Reaction / Q000379:methods; D019936:Probiotics / Q000032:analysis*; D012680:Sensitivity and Specificity; D013552:Swine / Q000382:microbiology*
chemical_list: D004269:DNA, Bacterial