The application of 19F magnetic resonance ex vivo imaging of three-dimensional cultured breast cancer cells to study the effect of delta-tocopherol.
The aim of this study was to determine the role of delta-tocopherol in breast cancer cell growth ex vivo. Human gland mammary adenocarcinoma (MCF-7) and human T-lymphoblastoid (CEM) cells were cultured in the presence of delta-tocopherol at various concentrations (0-750 microM) for 5 days. We have grown 3D ex vivo breast cancer cell cultures in the hollow fiber bioreactor (HFBR). (19)F magnetic resonance imaging (MRI) was used to evaluate oxygen concentration in the cell suspension and thus its viability.
Bartusik
Dorota
D
National Research Council Canada, Institute for Biodiagnostics (West), 3330 Hospital Drive NW, Calgary, Alta., Canada T2N 4N1. Dorota.Bartusik@nrc-cnrc.gc.ca
Tomanek
Boguslaw
B
Siluk
Danuta
D
Kaliszan
Roman
R
Fallone
Gino
G
D001943:Breast Neoplasms / Q000473:pathology*; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D005260:Female; D005461:Fluorine; D006801:Humans; D008279:Magnetic Resonance Imaging / Q000379:methods*; D010100:Oxygen / Q000032:analysis; D024505:Tocopherols / Q000494:pharmacology*
D005461:Fluorine; C479072:delta-tocopherol; D024505:Tocopherols; D010100:Oxygen