[The expression and relationship of vascular cell adhesion molecule-1 and P-selectin and microvessel density in hypopharyngeal carcinoma].
OBJECTIVE
To investigate the expression and significance of vascular cell adhesion molecule-1 (VCAM-1) and P-selectin in hypopharyngeal carcinoma, and explore the relationship of VCAM-1, P-selectin and microvessel density (MVD).


METHODS
Expression of VCAM-1 and P-selectin were detected by immunohistochemistry staining in 40 specimens of hypopharyngeal carcinoma and 10 specimens of normal mucosa of oral pharynx. MVD was assessed based on the expression of CD34.


RESULTS
VCAM-1 and P-selectin were detected in 26 out of 40 (65%) and 29 out of 40 (72.5%) respectively in hypopharyngeal carcinoma, but none that in normal mucosa (P<0.01). MVD in hypopharyngeal carcinoma was higher than that in normal mucosa and it was related to lymph node metastasis. MVD level was significantly higher in VCAM-1 and P-selectin-positive specimens than in negative ones (P<0.01), which also positively correlated with the expression of VCAM-1 and P-selectin (P<0.01). The expression of P-selectin positively correlated with VCAM-1 in hypopharyngeal carcinoma specimens with lymph node metastasis.


CONCLUSIONS
The higher expression of VCAM-1 and P-selectin play key roles in the invasion and metastasis of hypopharyngeal carcinoma and were correlated with vascularization.
Liang
Dapeng
D
Department of Otolaryngology Head and Neck Surgery, Qilu Hospital of Shandong University, Jinan, 250012, China.
Pan
Xinliang
X
Sun
Wenhai
W
Huang
Yichuan
Y
D000328:Adult; D000368:Aged; D002294:Carcinoma, Squamous Cell / Q000098:blood supply* / Q000378:metabolism / Q000473:pathology; D006801:Humans; D007012:Hypopharyngeal Neoplasms / Q000098:blood supply* / Q000378:metabolism / Q000473:pathology; D008207:Lymphatic Metastasis; D008297:Male; D055806:Microvessels; D008875:Middle Aged; D009367:Neoplasm Staging; D019007:P-Selectin / Q000378:metabolism*; D019010:Vascular Cell Adhesion Molecule-1 / Q000378:metabolism*
D019007:P-Selectin; C546937:SELP protein, human; D019010:Vascular Cell Adhesion Molecule-1