Reduction of sample evaporation in small volume microplate luminescence assays.
Luminescence detection has great potential for use in small volume assays for high-throughput applications. However, the need for automated injection of detection reagents dictates an open well format, making small volume luminescence assays very susceptible to sample evaporation effects. Here we describe a technique for reducing evaporation of small sample volumes that uses layering of silicone oil on solution surfaces but still allows the use of automated injection.
Gregory
Kalvin J
KJ
Sci-Tec Inc., Knoxville, TN 37932, USA. klvngrgry@hotmail.com
Sun
Yeh
Y
D008163:Luminescent Measurements / Q000379:methods*; D012827:Silicone Oils / Q000494:pharmacology; D014835:Volatilization
D012827:Silicone Oils