title: The effects of prenatal alcohol exposure on the morphological characteristics of spinal motoneurons.
abstract: BACKGROUND
  Clinical studies and research in animals have established that alcohol consumption during pregnancy produces irreversible developmental anomalies. Deficits in fine motor performance are often noted in infants diagnosed with fetal alcohol syndrome. However, the effects of alcohol on the spinal motoneurons have not been examined. In this study, the morphometric alterations in spinal motoneurons were assessed as a result of prenatal alcohol exposure.
  
  
  METHODS
  Pregnant Sprague Dawley rats were administered with 1.0 ml of 20% ethyl alcohol per 100 gm body weight via intraperitoneal injections, and unexposed rats served as controls. Rats were perfused through the left cardiac ventricle and a complete laminectomy was performed. Spinal cord sections from the L4-5 segments were cut serially and stained for cresyl fast violet. Sections were also subjected to TUNEL assay for detection of apoptosis. Observations were made between 1 and 4 weeks after birth.
  
  
  RESULTS
  Morphologic characteristics of motoneurons in the alcohol-exposed group of rats were altered. Counts and measurements revealed significant reduction in number and size of alcohol-exposed spinal motoneurons at all time points studied.
  
  
  CONCLUSIONS
  Prenatal exposure to alcohol showed cytotoxic effects whereby it adversely affected both motoneuron growth and differentiation in utero.
authors.affiliation: Department of Anatomy, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia. rosiepamela@um.edu.my
authors.forename: Pamela
authors.initials: P
authors.lastname: David
authors.forename: Krishnan
authors.initials: K
authors.lastname: Subramaniam
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D001835:Body Weight / Q000187:drug effects; D002492:Central Nervous System Depressants / Q000633:toxicity*; D004195:Disease Models, Animal; D000431:Ethanol / Q000633:toxicity*; D005260:Female; D063647:Fetal Alcohol Spectrum Disorders / Q000209:etiology / Q000473:pathology*; D008297:Male; D018811:Maternal Exposure; D009046:Motor Neurons / Q000187:drug effects* / Q000473:pathology; D011247:Pregnancy; D011297:Prenatal Exposure Delayed Effects / Q000139:chemically induced* / Q000473:pathology; D051381:Rats; D017207:Rats, Sprague-Dawley; D013127:Spinal Nerves / Q000187:drug effects* / Q000196:embryology / Q000473:pathology
chemical_list: D002492:Central Nervous System Depressants; D000431:Ethanol