Polygalasaponin G promotes neurite outgrowth of cultured neuron on myelin.
Myelin contains many axonal outgrowth inhibitory components which contribute to regeneration failure after neuronal injury in the mammalian central nervous system (CNS). In an attempt to develop small molecular agents to promote axonal outgrowth, we screened a compound library purified from traditional Chinese herbs, and found a small molecular compound polygalasaponin G (PS-G), extracted from Polygala japonica, which has a potent neurotrophic activity on PC12 cells and cultured cortical neurons. We reported, to our knowledge for the first time, that PS-G could promote neurite outgrowth of neurons cultured on the myelin substrates and inhibit the activation of RhoA. Thus, our results could represent a therapeutic approach to improve axon regeneration after CNS injuries.
Xu
Xiao-Hui
XH
Institute of Neuroscience and Key Laboratory of Molecular Neurobiology, Ministry of Education, Second Military Medical University, Shanghai 200433, China.
Zhou
Jian-Feng
JF
Li
Ting-Zhao
TZ
Zhang
Zhao-Huan
ZH
Shan
Lei
L
Xiang
Zheng-Hua
ZH
Yu
Zhong-Wang
ZW
Zhang
Wei-Dong
WD
He
Cheng
C
D000818:Animals; D000831:Animals, Newborn; D002478:Cells, Cultured; D002531:Cerebellum / Q000166:cytology / Q000502:physiology; D004305:Dose-Response Relationship, Drug; D020287:In Situ Nick-End Labeling / Q000379:methods; D009185:Myelin Proteins / Q000494:pharmacology; D009186:Myelin Sheath / Q000502:physiology*; D020932:Nerve Growth Factor / Q000494:pharmacology; D016501:Neurites / Q000187:drug effects*; D055495:Neurogenesis / Q000187:drug effects / Q000502:physiology; D009474:Neurons / Q000166:cytology*; D000070798:Nogo Proteins; D016716:PC12 Cells; D031801:Polygala / Q000737:chemistry*; D051381:Rats; D012503:Saponins / Q000737:chemistry / Q000494:pharmacology*; D020742:rhoA GTP-Binding Protein / Q000378:metabolism
D009185:Myelin Proteins; D000070798:Nogo Proteins; C000603197:Rtn4 protein, rat; D012503:Saponins; C543171:polygalasaponin G; D020932:Nerve Growth Factor; D020742:rhoA GTP-Binding Protein