title: Low mass cutoff evasion with q(z) value optimization in ion trap.
abstract: An ion trap is a powerful analyzer because of its high resolution, high sensitivity, and multistage mass analysis (MS(n)) capabilities. Multiple fragmentation analysis provides useful information regarding peptide sequence and biomolecular structure; however, this approach is limited by an inherent low mass cutoff (LMCO) derived from collision-induced dissociation (CID). To avoid the LMCO for application of an ion trap to iTRAQ, we optimized the q(z) value, which is a parameter that is proportional to the applied fundamental AC radio frequency voltage of a tandem mass spectrometry (MS/MS) event. Considering that many ion trap MS analyses employ CID as the MS/MS method, this method can be a practical one without any instrumental changes.
authors.affiliation: School of Chemical and Biological Engineering and Interdisciplinary Program for Biochemical Engineering and Biotechnology, Institute of Bioengineering, Seoul National University, Seoul 110-744, Republic of Korea.
authors.forename: Yung-Hun
authors.initials: YH
authors.lastname: Yang
authors.forename: Kwangwon
authors.initials: K
authors.lastname: Lee
authors.forename: Kyoung-Soon
authors.initials: KS
authors.lastname: Jang
authors.forename: Yun-Gon
authors.initials: YG
authors.lastname: Kim
authors.forename: Sung-Hee
authors.initials: SH
authors.lastname: Park
authors.forename: Chang-Soo
authors.initials: CS
authors.lastname: Lee
authors.forename: Byung-Gee
authors.initials: BG
authors.lastname: Kim
mesh_terms: D053719:Tandem Mass Spectrometry / Q000379:methods*