p65/RelA binds and activates the beclin 1 promoter.
We unveiled novel p65/RelA consensus sites in the promoter of the beclin 1 gene and demonstrate that p65/RelA positively modulates canonical autophagy in various human cell lines both under basal conditions and upon induction by ceramide. Interestingly, we find that T cell receptor-dependent activation of Jurkat cells triggers an increase in the binding of p65/RelA to the beclin 1 promoter accompanied by enhanced autophagy, suggesting that p65/RelA could regulate T-cell activation and homeostasis through autophagy.
Copetti
Tamara
T
LNCIB Laboratorio Nazionale Consorzio Interuniversitario Biotecnologie AREA Science Park, Trieste, Italy.
Demarchi
Francesca
F
Schneider
Claudio
C
D000818:Animals; D051017:Apoptosis Regulatory Proteins / Q000235:genetics*; D001343:Autophagy; D000071186:Beclin-1; D001665:Binding Sites; D006801:Humans; D019169:Jurkat Cells; D008565:Membrane Proteins / Q000235:genetics*; D051379:Mice; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D015398:Signal Transduction; D051996:Transcription Factor RelA / Q000378:metabolism*
D051017:Apoptosis Regulatory Proteins; C491997:BECN1 protein, human; D000071186:Beclin-1; D008565:Membrane Proteins; D051996:Transcription Factor RelA