title: Product profile of PEN3: the last unexamined oxidosqualene cyclase in Arabidopsis thaliana.
abstract: The triterpene product profile is reported for At5g36150 (PEN3), the last unexamined oxidosqualene cyclase in the reference plant Arabidopsis thaliana. PEN3 makes tirucalla-7,24-dien-3beta-ol ( approximately 85%) and several minor products. Also discussed are the unexpectedly facile convergent evolution of another Arabidopsis tirucalladienol synthase (LUP5), mechanistic origins of the 20S configuration, and active-site remodeling necessary to accommodate the 17alpha side chain. This work marks the first completed functional characterization of all triterpene synthases in a higher plant.
authors.affiliation: Department of Chemistry, Rice University, Houston, Texas 77005, USA.
authors.forename: Pietro
authors.initials: P
authors.lastname: Morlacchi
authors.forename: William K
authors.initials: WK
authors.lastname: Wilson
authors.forename: Quanbo
authors.initials: Q
authors.lastname: Xiong
authors.forename: Aparna
authors.initials: A
authors.lastname: Bhaduri
authors.forename: Diana
authors.initials: D
authors.lastname: Sttivend
authors.forename: Mariya D
authors.initials: MD
authors.lastname: Kolesnikova
authors.forename: Seiichi P T
authors.initials: SP
authors.lastname: Matsuda
mesh_terms: D018528:ATP-Binding Cassette Transporters / Q000378:metabolism*; D017360:Arabidopsis / Q000201:enzymology*; D029681:Arabidopsis Proteins / Q000378:metabolism*; D003500:Cyclization; D006801:Humans; D019751:Intramolecular Transferases / Q000378:metabolism*; D015394:Molecular Structure; D014315:Triterpenes / Q000138:chemical synthesis* / Q000378:metabolism*
chemical_list: D018528:ATP-Binding Cassette Transporters; D029681:Arabidopsis Proteins; C510424:PDR8 protein, Arabidopsis; D014315:Triterpenes; D019751:Intramolecular Transferases; C021924:lanosterol synthase