[Comparison of cytotoxicity between chemical retraction agents on human gingival fibroblasts in vitro].
OBJECTIVE
To choose the best retraction agent for the clinic by evaluating cytotoxic effects of six chemical retraction agents on human gingival fibroblasts (HGF) in vitro.


METHODS
HGF were treated with six chemical retraction agents which included 20% Al2(SO4)3, 5% Al2(SO4)3, 15.5% Fe2(SO4)3, 13.3% Fe2(SO4)3, 0.1% HCl-epinephrine and 0.01% HCl-epinephrine. The damage of direct contact and proliferation inhibit were observed by methyl thiazolyl tetrazolium (MTT) colorimetric assay, the ultra structure was observed under transmission electron microscope (TEM).


RESULTS
All of chemical retraction agents caused cell damage and proliferation inhibit. The ability of cytotoxic effect increased in an order of 0.01% HCl-epinephrine<0.1% HCl-epinephrine<5% Al2(SO4)3<20% Al2(SO4)3<Fe2(SO4)3. There was no statistically significant difference between 13.3% Fe2(SO4)3 group and 15.5% Fe2(SO4)3 group. At HCl-epinephrine group and 5% Al2(SO4)3 group, the number of organelles were decreased, mitochondria and endoplasmic reticulum were swollen. At 20% Al2(SO4)3 group, organelles swelling and decreased number of organelles became more severe, the nuclear chromatin was unevenly distributed. Cell degeneration could be made by Fe2(SO4)3.


CONCLUSIONS
0.01% HCl-epinephrine was the least toxic gingival retraction agents, 13.3% Fe2(SO4)3 and 15.5% Fe2(SO4)3 are found to have the strongest cytotoxic effect on cell cultures.
Liu
Jian
J
College of Stomatology, Binzhou Medical University, Binzhon 256603, China.
Zhang
Xiao-Ming
XM
Hao
Peng-Jie
PJ
Hui
Min
M
Yu
Huan-Ying
HY
D000818:Animals; D004837:Epinephrine; D005347:Fibroblasts; D005881:Gingiva; D006801:Humans; D066298:In Vitro Techniques
D004837:Epinephrine