title: [HBVcccDNA of patients with hepatitis B in the serum, PBMC and liver tissue distribution].
abstract: OBJECTIVE
  Hepatitis B virus covalently closed circular DNA in the serum of patients with hepatitis B, peripheral mononuclear cells (PBMC) and liver tissue distribution.
  
  
  METHODS
  Serum HBV DNA > 10(5) copies/ml 50 cases of hepatitis B patients, serum HBV DNA < 10(5) copies/ml 30 cases of hepatitis B patients, patients with fatty liver (hepatitis B) 20 cases, using real-time quantitative polymerase chain reaction for detection of serum, and liver tissue in PBMC HBVcccDNA the existence.
  
  
  RESULTS
  Of 50 serum HBV DNA > 10(5) copies/ml cccDNA the serum specimens were 28 cases, 56% detection rate, 29 cases were PBMC HBVcccDNA, 58% detection rate, liver tissue HBVcccDNA were 44 cases, 88% detection rate, serum, the PBMC were detected in liver tissue were significant differences in P < 0.005, compared serum PBMC were no significant differences in P > 0.005. 30 serum HBV DNA < 10(5) copies/ml the serum specimens, PBMC cccDNA detected two cases were 6.67% detection rate, liver tissue cccDNA were six cases 20% detection rate, serum, PBMC, were among the liver tissue was not significantly different P > 0.005. 20 in the serum of patients with fatty liver, liver tissue in PBMCwere not detected HBVcccDNA.
  
  
  CONCLUSIONS
  HBVcccDNA mainly in the liver of patients with hepatitis B, hepatitis B patients and also cccDNA PBMC in the presence of liver tissue but a few of many.
authors.affiliation: The Sixth People's Hospital of Hangzhou Affiliated to Zhejiang Chinese Medial University, Hangzhou 310014, China. da-an-ren@163.com
authors.forename: Yong-le
authors.initials: YL
authors.lastname: Zhang
authors.forename: Dai
authors.initials: D
authors.lastname: Xu
authors.forename: Xiao-xing
authors.initials: XX
authors.lastname: Wu
authors.forename: Jing
authors.initials: J
authors.lastname: Wu
authors.forename: Xin-yi
authors.initials: XY
authors.lastname: Shao
authors.forename: Hong
authors.initials: H
authors.lastname: Chen
authors.forename: Qin
authors.initials: Q
authors.lastname: Zhang
mesh_terms: D000328:Adult; D004270:DNA, Circular / Q000097:blood*; D004279:DNA, Viral / Q000097:blood*; D005234:Fatty Liver / Q000097:blood / Q000821:virology; D005260:Female; D006509:Hepatitis B / Q000097:blood / Q000821:virology*; D006515:Hepatitis B virus / Q000235:genetics* / Q000502:physiology; D006801:Humans; D008099:Liver / Q000821:virology*; D008297:Male
chemical_list: D004270:DNA, Circular; D004279:DNA, Viral