title: Synthesis and noncovalent protein conjugation of linear-hyperbranched PEG-poly(glycerol) alpha,omega(n)-telechelics.
abstract: Linear-hyperbranched, heterobifunctional alpha,omega(n) telechelic block copolymers consisting of a linear poly(ethylene glycol) (PEG) chain and a hyperbranched polyglycerol (PG) block have been prepared in five steps, using a protected amino-functional initiator. The polyfunctionality omega(n) (OH groups) can be adjusted by the degree of polymerization (DP(n)) of the polyglycerol block. Subsequent introduction of a single biotin unit by amidation in alpha-position permitted noncovalent bioconjugation with avidin.
authors.affiliation: Institute of Organic Chemistry, Johannes Gutenberg University Mainz, Duesbergweg 10-14, 55099 Mainz, Germany.
authors.forename: Frederik
authors.initials: F
authors.lastname: Wurm
authors.forename: Johannes
authors.initials: J
authors.lastname: Klos
authors.forename: Hans Joachim
authors.initials: HJ
authors.lastname: Räder
authors.forename: Holger
authors.initials: H
authors.lastname: Frey
mesh_terms: D019921:Biotinylation; D005990:Glycerol / Q000138:chemical synthesis / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000138:chemical synthesis / Q000737:chemistry*; D011108:Polymers / Q000138:chemical synthesis / Q000737:chemistry*; D011506:Proteins / Q000737:chemistry* / Q000627:therapeutic use
chemical_list: D011108:Polymers; D011506:Proteins; C043941:polyglycerol; D011092:Polyethylene Glycols; D005990:Glycerol