title: Potassium restriction, high protein intake, and metabolic acidosis increase expression of the glutamine transporter SNAT3 (Slc38a3) in mouse kidney.
abstract: Kidneys produce ammonium to buffer and excrete acids through metabolism of glutamine. Expression of the glutamine transporter Slc38a3 (SNAT3) increases in kidney during metabolic acidosis (MA), suggesting a role during ammoniagenesis. Potassium depletion and high dietary protein intake are known to elevate renal ammonium excretion. In this study, we examined SNAT3, phosphate-dependent glutaminase (PDG), and phosphoenolpyruvate carboxykinase (PEPCK) regulation during a control (0.36%) or low-K(+) (0.02%) diet for 7 or 14 days or a control (20%) or high-protein (50%) diet for 7 days. MA was induced in control and low-K(+) groups by addition of NH(4)Cl. Urinary ammonium excretion increased during MA, after 14-day K(+) restriction alone, and during high protein intake. SNAT3, PDG, and PEPCK mRNA abundance were elevated during MA and after 14-day K(+) restriction but not during high protein intake. SNAT3 protein abundance was enhanced during MA (both control and low K(+)), after 14-day low-K(+) treatment alone, and during high protein intake. Seven-day dietary K(+) depletion alone had no effect. Immunohistochemistry showed SNAT3 staining in earlier parts of the proximal tubule during 14-day K(+) restriction with and without NH(4)Cl treatment and during high protein intake. In summary, SNAT3, PDG, and PEPCK mRNA expression were congruent with urinary ammonium excretion during MA. Chronic dietary K(+) restriction, high protein intake, and MA enhance ammoniagenesis, an effect that may involve enhanced SNAT3 mRNA and protein expression. Our data suggest that SNAT3 plays an important role as the glutamine uptake mechanism in ammoniagenesis under these conditions.
authors.affiliation: Institute of Physiology, Zurich Center for Integrative Human Physiology, University of Zurich, Zurich, Switzerland.
authors.forename: Stephanie M
authors.initials: SM
authors.lastname: Busque
authors.forename: Carsten A
authors.initials: CA
authors.lastname: Wagner
mesh_terms: D000138:Acidosis / Q000139:chemically induced / Q000378:metabolism*; D026921:Amino Acid Transport Systems, Neutral / Q000235:genetics / Q000378:metabolism*; D000643:Ammonium Chloride; D000818:Animals; D002364:Caseins / Q000378:metabolism*; D004195:Disease Models, Animal; D005972:Glutaminase / Q000378:metabolism; D007668:Kidney / Q000378:metabolism*; D007687:Kidney Tubules, Proximal / Q000378:metabolism; D008297:Male; D051379:Mice; D010729:Phosphoenolpyruvate Carboxykinase (GTP) / Q000378:metabolism; D011191:Potassium Deficiency / Q000209:etiology / Q000378:metabolism*; D017419:Potassium, Dietary / Q000378:metabolism*; D000644:Quaternary Ammonium Compounds / Q000378:metabolism* / Q000652:urine; D012333:RNA, Messenger / Q000378:metabolism; D013997:Time Factors; D015854:Up-Regulation
chemical_list: D026921:Amino Acid Transport Systems, Neutral; D002364:Caseins; D017419:Potassium, Dietary; D000644:Quaternary Ammonium Compounds; D012333:RNA, Messenger; C403222:system N protein 1; D000643:Ammonium Chloride; D005972:Glutaminase; D010729:Phosphoenolpyruvate Carboxykinase (GTP)