title: Fluorescence lifetime imaging of quantum dot labeled DNA microarrays.
abstract: Quantum dot (QD) labeling combined with fluorescence lifetime imaging microscopy is proposed as a powerful transduction technique for the detection of DNA hybridization events. Fluorescence lifetime analysis of DNA microarray spots of hybridized QD labeled target indicated a characteristic lifetime value of 18.8 ns, compared to 13.3 ns obtained for spots of free QD solution, revealing that QD labels are sensitive to the spot microenvironment. Additionally, time gated detection was shown to improve the microarray image contrast ratio by 1.8, achieving femtomolar target sensitivity. Finally, lifetime multiplexing based on Qdot525 and Alexa430 was demonstrated using a single excitation-detection readout channel.
authors.affiliation: School of Physics and Astronomy, The University of Edinburgh, The King's Buildings, West Mains Road, Edinburgh EH9 3JZ, Scotland, UK.
authors.forename: Gerard
authors.initials: G
authors.lastname: Giraud
authors.affiliation: Division of Pathway Medicine, Medical School, The University of Edinburgh, Chancellor's Building, Little France Crescent, Edinburgh EH16 4SB, Scotland, UK.
authors.forename: Holger
authors.initials: H
authors.lastname: Schulze
authors.affiliation: Division of Pathway Medicine, Medical School, The University of Edinburgh, Chancellor's Building, Little France Crescent, Edinburgh EH16 4SB, Scotland, UK.
authors.forename: Till T
authors.initials: TT
authors.lastname: Bachmann
authors.affiliation: Division of Pathway Medicine, Medical School, The University of Edinburgh, Chancellor's Building, Little France Crescent, Edinburgh EH16 4SB, Scotland, UK.
authors.forename: Colin J
authors.initials: CJ
authors.lastname: Campbell
authors.affiliation: School of Chemistry , The University of Edinburgh , Joseph Black Building, West Mains Road, Edinburgh, EH9 3JJ, Scotland, UK.
authors.forename: Andrew R
authors.initials: AR
authors.lastname: Mount
authors.affiliation: Division of Pathway Medicine, Medical School, The University of Edinburgh, Chancellor's Building, Little France Crescent, Edinburgh EH16 4SB, Scotland, UK.
authors.forename: Peter
authors.initials: P
authors.lastname: Ghazal
authors.affiliation: Division of Pathway Medicine, Medical School, The University of Edinburgh, Chancellor's Building, Little France Crescent, Edinburgh EH16 4SB, Scotland, UK.
authors.forename: Mizanur R
authors.initials: MR
authors.lastname: Khondoker
authors.affiliation: Division of Pathway Medicine, Medical School, The University of Edinburgh, Chancellor's Building, Little France Crescent, Edinburgh EH16 4SB, Scotland, UK.
authors.forename: Alan J
authors.initials: AJ
authors.lastname: Ross
authors.affiliation: Division of Pathway Medicine, Medical School, The University of Edinburgh, Chancellor's Building, Little France Crescent, Edinburgh EH16 4SB, Scotland, UK.
authors.forename: Stuart W J
authors.initials: SWJ
authors.lastname: Ember
authors.affiliation: School of Chemistry , The University of Edinburgh , Joseph Black Building, West Mains Road, Edinburgh, EH9 3JJ, Scotland, UK.
authors.forename: Ilenia
authors.initials: I
authors.lastname: Ciani
authors.affiliation: School of Chemistry , The University of Edinburgh , Joseph Black Building, West Mains Road, Edinburgh, EH9 3JJ, Scotland, UK.
authors.forename: Chaker
authors.initials: C
authors.lastname: Tlili
authors.affiliation: Institute of Integrated Systems, School of Engineering, The University of Edinburgh, The King's Buildings, West Mains Road, Edinburgh EH9 3JF, Scotland, UK.
authors.forename: Anthony J
authors.initials: AJ
authors.lastname: Walton
authors.affiliation: Institute of Integrated Systems, School of Engineering, The University of Edinburgh, The King's Buildings, West Mains Road, Edinburgh EH9 3JF, Scotland, UK.
authors.forename: Jonathan G
authors.initials: JG
authors.lastname: Terry
authors.affiliation: School of Physics and Astronomy, The University of Edinburgh, The King's Buildings, West Mains Road, Edinburgh EH9 3JZ, Scotland, UK.|National Physical Laboratory, Hampton Road, Teddington, Middlesex TW11 0LW, UK.
authors.forename: Jason
authors.initials: J
authors.lastname: Crain
mesh_terms: D003587:Cytomegalovirus / Q000235:genetics; D004247:DNA / Q000737:chemistry / Q000378:metabolism*; D004279:DNA, Viral / Q000737:chemistry / Q000378:metabolism; D016174:Hepacivirus / Q000235:genetics; D006801:Humans; D008856:Microscopy, Fluorescence; D009693:Nucleic Acid Hybridization; D020411:Oligonucleotide Array Sequence Analysis; D045663:Quantum Dots; D012367:RNA, Viral / Q000737:chemistry / Q000378:metabolism
chemical_list: D004279:DNA, Viral; D012367:RNA, Viral; D004247:DNA