[Measurement of airway resistance and reactivity in guinea pigs using double-chamber plethysmography].
OBJECTIVE
To establish a method for measurement of airway resistance (sRaw) and reactivity in guinea pigs.


METHODS
Methacholine spray at gradient concentrations was given to guinea pigs. PC100 was defined as the concentration of methacholine when the sRaw doubled in the guinea pigs using a double-chamber plethysmograph. The time for the recovery of PC100 resistance to baseline levels was measured. The sRaw and PC100 were measured twice on days 1 and 15 (4 time points) in the guinea pigs before and after OVA challenge.


RESULTS
PC100 in a normal guinea pig airway was shown to recover the baseline level within 1 h. Double-chamber plethysmographical measurement of the sRaw and PC100 in normal guinea pigs did not show significant differences between the time points [sRaw: 3.25-/+0.67, 3.33-/+0.58, 3.30-/+0.56, and 3.32-/+0.75 cm H2O.s; log2PC100: 8.48-/+0.94, 8.64-/+1.04, 8.56-/+0.67, and 8.64-/+0.60, respectively, P>0.05]. The sRaw and airway reactivity were significantly increased in guinea pigs challenged with OVA [sRaw: 7.08-/+1.82 vs 2.87-/+0.53 cmH2O.s, P<0.01; log2PC100: 6.64-/+1.26 vs 8.48-/+1.17, P<0.01].


CONCLUSIONS
A double-chamber plethysmography for measurement of sRaw and airway reactivity in guinea pig is established successfully.
Yao
Wei-min
WM
State Key Laboratory of Respiratory Disease, Guangzhou Medical College, First Affiliated Hospital, Guangzhou 510120, China. yao7722@ yahoo.com.cn
Lai
Ke-fang
KF
Luo
Yuan-ming
YM
Liu
Chun-li
CL
Chen
Ru-chong
RC
Luo
Wei
W
Zhong
Nan-shan
NS
D000403:Airway Resistance; D000818:Animals; D001249:Asthma / Q000139:chemically induced / Q000503:physiopathology; D016535:Bronchial Hyperreactivity / Q000209:etiology / Q000503:physiopathology*; D006168:Guinea Pigs; D008297:Male; D016210:Methacholine Chloride; D010991:Plethysmography / Q000295:instrumentation / Q000379:methods*; D011897:Random Allocation
D016210:Methacholine Chloride