title: A novel role of catalase in detoxification of peroxynitrite in S. cerevisiae.
abstract: The biological targets of peroxynitrite toxicity include wide array of biomolecules. Although several enzymes are found to be important components of cellular defense against peroxynitrite, the complete scenario is not totally understood. Yeast flavohemoglobin (YHB) and glutathione-dependent formaldehyde dehydrogenase (GS-FDH) confers resistance against nitric oxide and related reactive nitrogen species. In the present study, when subtoxic dose of peroxynitrite was applied to wild type, Deltayhb1 and Deltasfa1 strains of Saccharomyces cerevisiae, induction of cytosolic catalase was found at activity as well as gene expression level in mutants but not in wild type. Such induction was not due to intracellular reactive oxygen species (ROS) formation. Our in vitro studies confirmed the role of catalase in protection against peroxynitrite-mediated oxidation and nitration and also in peroxynitrite catabolism. This report is first of its kind regarding the novel role of catalase in peroxynitrite detoxification in Deltayhb1 and Deltasfa1 strains of S. cerevisiae.
authors.affiliation: Department of Biochemistry, University College of Science, University of Calcutta, 35, Ballygunge Circular Road, Kolkata 700019, India.
authors.forename: Rupam
authors.initials: R
authors.lastname: Sahoo
authors.forename: Arindam
authors.initials: A
authors.lastname: Bhattacharjee
authors.forename: Uddalak
authors.initials: U
authors.lastname: Majumdar
authors.forename: Sougata Sinha
authors.initials: SS
authors.lastname: Ray
authors.forename: Tanmay
authors.initials: T
authors.lastname: Dutta
authors.forename: Sanjay
authors.initials: S
authors.lastname: Ghosh
mesh_terms: D000445:Aldehyde Oxidoreductases / Q000235:genetics; D019730:Carbon-Sulfur Ligases / Q000235:genetics; D002374:Catalase / Q000235:genetics / Q000502:physiology*; D049308:Dioxygenases / Q000235:genetics; D006420:Hemeproteins / Q000235:genetics; D030421:Peroxynitrous Acid / Q000378:metabolism* / Q000633:toxicity; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics
chemical_list: D006420:Hemeproteins; D029701:Saccharomyces cerevisiae Proteins; D030421:Peroxynitrous Acid; D002374:Catalase; D049308:Dioxygenases; C101796:YHB1 protein, S cerevisiae; D000445:Aldehyde Oxidoreductases; C087006:formaldehyde dehydrogenase, glutathione-independent; D019730:Carbon-Sulfur Ligases; C447825:glutathione-dependent formaldehyde-activating enzyme