title: [Effect of sequential intratumoral injection of xenogeneic antigens for immunotherapy in immunized mice bearing S180 tumor].
abstract: OBJECTIVE
  To investigate the therapeutic effect of sequential intratumoral injection of xenogeneic antigens in immunized tumor-bearing mice.
  
  
  METHODS
  Sequential intratumoral injection of the xenoantigens was performed in immunized mice bearing S180 tumor. The tumor size changes were observed, and the tumor-infiltrating lymphocytes (TIL) including CD3+CD4+T, CD3+CD8+T, and CD3+CD4+CD25+T lymphocytes were counted with flow cytometry. The concentrations of IL-2 and TNF-alpha in the tumor was measured using ELISA.
  
  
  RESULTS
  No significant difference was found in the number of CD3+T lymphocytes in the TILs between different groups. After the immunotherapy, the percentages of CD3+CD4+T, CD3+CD8+T and CD3+CD4+CD25+T lymphocytes were 54%, 22% and 2.91%, respectively, with the CD4+/CD8+ ratio of 2.49, significantly different from that in the control group (P<0.05). The concentrations of IL-2 and TNF-alpha were 100.61 pg/ml and 54.114 pg/ml, respectively, significantly different from those in the control group (P<0.05).
  
  
  CONCLUSIONS
  Sequential intratumoral injection of heteragenetic antigena can significantly increase the amount of effector cells and cytokines in the micro-environment of the tumor, and decrease the expression of T regulatory.
authors.affiliation: Oncology Center, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, China.
authors.forename: Li-bin
authors.initials: LB
authors.lastname: Sun
authors.forename: Ji-ren
authors.initials: JR
authors.lastname: Zhang
authors.forename: Xi-gang
authors.initials: XG
authors.lastname: Hu
authors.forename: Yan-tao
authors.initials: YT
authors.lastname: Mao
mesh_terms: D000818:Animals; D015478:Antigens, Heterophile / Q000276:immunology*; D016516:CD4-CD8 Ratio; D005260:Female; D007167:Immunotherapy / Q000379:methods*; D016246:Lymphocytes, Tumor-Infiltrating / Q000166:cytology*; D008297:Male; D051379:Mice; D011897:Random Allocation; D012510:Sarcoma 180 / Q000276:immunology / Q000628:therapy*; D013291:Streptococcus / Q000276:immunology*
chemical_list: D015478:Antigens, Heterophile