title: Proteome analysis of haptoglobin in cerebrospinal fluid of neuromyelitis optica.
abstract: Neuromyelitis optica (NMO) is an inflammatory demyelinating disease with generally poor prognosis that selectively targets optic nerves and spinal cord. Although diagnostic criteria for NMO are available, there is still a need for biomarkers, predicting disease development and progression to improve individually tailored treatment. CSF proteins were separated by two-dimensional electrophoresis and identified by matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS). The interaction between these proteins was further analyzed by Pathway Studio software. Seven protein spots in CSF were significantly altered in NMO patients compared with controls. Identification made by mass spectrometry revealed that the most significant protein was haptoglobin, which was increased in the NMO gels. The subsequent ELISA test were performed to validate it, which confirmed the results of proteomic analysis. Protein network was built, which showed some biological interactions among the seven proteins. These results support a correlation between the level of haptoglobin and NMO. Haptoglobin may be a potential useful biomarker for diagnosis or a medicine target for treatment of NMO.
authors.affiliation: Institute of Biochemistry and Molecular Biology, School of Medicine, Shandong University, Shandong, People's Republic of China.
authors.forename: Shumei
authors.initials: S
authors.lastname: Bai
authors.forename: Shilian
authors.initials: S
authors.lastname: Liu
authors.forename: Xuxiao
authors.initials: X
authors.lastname: Guo
authors.forename: Zhaoyu
authors.initials: Z
authors.lastname: Qin
authors.forename: Banqin
authors.initials: B
authors.lastname: Wang
authors.forename: Xiaohong
authors.initials: X
authors.lastname: Li
authors.forename: Yanjiang
authors.initials: Y
authors.lastname: Qin
mesh_terms: D000328:Adult; D015415:Biomarkers / Q000134:cerebrospinal fluid*; D019295:Computational Biology; D015180:Electrophoresis, Gel, Two-Dimensional; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006242:Haptoglobins / Q000134:cerebrospinal fluid* / Q000235:genetics*; D006801:Humans; D008297:Male; D009471:Neuromyelitis Optica / Q000134:cerebrospinal fluid*; D025941:Protein Interaction Mapping; D040901:Proteomics; D012984:Software; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D018709:Statistics, Nonparametric
chemical_list: D015415:Biomarkers; D006242:Haptoglobins