title: The adsorption of Pseudomonas aeruginosa bacteriophage phiKMV is dependent on expression regulation of type IV pili genes.
abstract: Pseudomonas aeruginosa bacteriophage phiKMV requires type IV pili for infection, as observed from the phenotypic characterization and phage adsorption assays on a phage infection-resistant host strain mutant. A cosmid clone library of the host (P. aeruginosa PAO1) genomic DNA was generated and used to select for a clone that was able to restore phiKMV infection in the resistant mutant. This complementing cosmid also re-established type IV pili-dependent twitching motility. The correlation between bacteriophage phiKMV infectivity and type IV pili, along with its associated twitching motility, was confirmed by the resistance of a P. aeruginosa PAO1DeltapilA mutant to the phage. Subcloning of the complementing cosmid and further phage infection analysis and motility assays suggests that a common regulatory mechanism and/or interaction between the ponA and pilMNOPQ gene products are essential for bacteriophage phiKMV infectivity.
authors.affiliation: Division of Gene Technology, Katholieke Universiteit Leuven, Leuven, Belgium.
authors.forename: Andrew
authors.initials: A
authors.lastname: Chibeu
authors.forename: Pieter-Jan
authors.initials: PJ
authors.lastname: Ceyssens
authors.forename: Kirsten
authors.initials: K
authors.lastname: Hertveldt
authors.forename: Guido
authors.initials: G
authors.lastname: Volckaert
authors.forename: Pierre
authors.initials: P
authors.lastname: Cornelis
authors.forename: Sandra
authors.initials: S
authors.lastname: Matthijs
authors.forename: Rob
authors.initials: R
authors.lastname: Lavigne
mesh_terms: D010861:Fimbriae, Bacterial / Q000235:genetics / Q000502:physiology*; D015723:Gene Library; D005798:Genes, Bacterial; D005816:Genetic Complementation Test; D008124:Locomotion; D017105:Pseudomonas Phages / Q000502:physiology*; D011550:Pseudomonas aeruginosa / Q000235:genetics / Q000502:physiology / Q000821:virology*; D053585:Virus Attachment