title: Antioxidant and immunity activity of water extract and crude polysaccharide from Ficus carica L. fruit.
abstract: The antioxidative activities of water extract (WE) and crude hot-water soluble polysaccharide (PS) from Ficus carica L. fruit were investigated using various assays in vitro, including scavenging abilities on DPPH, superoxide and hydroxyl radicals and reducing power. The immunity activities of PS were evaluated using the carbon clearance test and serum hemolysin analysis in mice. In addition, total phenolics and flavonoids contents were also determined. Both WE and PS have notable scavenging activities on DPPH with the EC(50) values of 0.72 and 0.61 mg/ml, respectively. The PS showed higher scavenging activity than WE on superoxide radical (EC(50), 0.95 mg/ml) and hydroxyl anion radical (scavenging rate 43.4% at concentration of 4 mg/ml). The PS (500 mg/kg) also has a significant increase in the clearance rate of carbon particles and serum hemolysin level of normal mice. The results indicate that both WE and PS might be applicable in healthy medicine and food industry.
authors.affiliation: School of Chemistry and Chemical Engineering, Jiangsu University, Zhenjiang 212013, People's Republic of China. zjyxm2003@hotmail.com
authors.forename: Xiao-Ming
authors.initials: XM
authors.lastname: Yang
authors.forename: Wei
authors.initials: W
authors.lastname: Yu
authors.forename: Zhong-Ping
authors.initials: ZP
authors.lastname: Ou
authors.forename: Hai-le
authors.initials: HL
authors.lastname: Ma
authors.forename: Wei-Ming
authors.initials: WM
authors.lastname: Liu
authors.forename: Xue-Lin
authors.initials: XL
authors.lastname: Ji
mesh_terms: D000818:Animals; D000917:Antibody Formation / Q000187:drug effects*; D000975:Antioxidants / Q000032:analysis / Q000494:pharmacology*; D001713:Biphenyl Compounds; D002244:Carbon / Q000378:metabolism; D030681:Ficus; D005419:Flavonoids / Q000032:analysis / Q000494:pharmacology; D005638:Fruit; D006460:Hemolysin Proteins / Q000097:blood; D017665:Hydroxyl Radical / Q000378:metabolism; D017737:Macrophages, Peritoneal / Q000187:drug effects*; D051379:Mice; D010587:Phagocytosis / Q000187:drug effects*; D010636:Phenols / Q000032:analysis / Q000494:pharmacology; D010851:Picrates; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D011134:Polysaccharides / Q000737:chemistry / Q000494:pharmacology*; D011897:Random Allocation; D013481:Superoxides / Q000378:metabolism
chemical_list: D000975:Antioxidants; D001713:Biphenyl Compounds; D005419:Flavonoids; D006460:Hemolysin Proteins; D010636:Phenols; D010851:Picrates; D010936:Plant Extracts; D011134:Polysaccharides; D013481:Superoxides; D017665:Hydroxyl Radical; D002244:Carbon; C004931:1,1-diphenyl-2-picrylhydrazyl