title: Role of toll-like receptor 3, RIG-I, and MDA5 in the expression of mesothelial IL-8 induced by viral RNA.
abstract: Interleukin-8 (IL-8) is a chemokine that has been shown to be a potent chemoattractant for polymorphonuclear neutrophils from the vascular compartment into the pleural space during infectious pleural effusions. Mesothelial cells express the viral receptors Toll-like receptor 3 (TLR3), RIG-I, and MDA5. Activation of these receptors by viral RNA exemplified by poly (I:C) RNA leads to a time- and dose-dependent increase of mesothelial IL-8 synthesis. To show the specific effect of viral receptors, knockdown experiments with short interfering RNA specific for TLR3, RIG-I and MDA5 were performed. This novel finding of functional expression of these viral sensors on human mesothelial cells may indicate a novel link between viral infections and mesothelial inflammation and indicates a pathophysiologic role of viral receptors in these processes.
authors.affiliation: Medical Policlinic, Ludwig-Maximilians-University, Pettenkoferstrasse 8a, 80336 Munich, Germany. Markus.Woernle@med.uni-muenchen.de
authors.forename: Markus
authors.initials: M
authors.lastname: Wörnle
authors.forename: Matthias
authors.initials: M
authors.lastname: Sauter
authors.forename: Kathrin
authors.initials: K
authors.lastname: Kastenmüller
authors.forename: Andrea
authors.initials: A
authors.lastname: Ribeiro
authors.forename: Thomas
authors.initials: T
authors.lastname: Mussack
authors.forename: Roland
authors.initials: R
authors.lastname: Ladurner
authors.forename: Thomas
authors.initials: T
authors.lastname: Sitter
mesh_terms: D002478:Cells, Cultured; D000071457:DEAD Box Protein 58; D053487:DEAD-box RNA Helicases / Q000502:physiology*; D004847:Epithelial Cells / Q000187:drug effects / Q000378:metabolism*; D006801:Humans; D000072640:Interferon-Induced Helicase, IFIH1; D016209:Interleukin-8 / Q000096:biosynthesis* / Q000235:genetics; D011070:Poly I-C / Q000494:pharmacology*; D012367:RNA, Viral / Q000494:pharmacology*; D011971:Receptors, Immunologic; D051196:Toll-Like Receptor 3 / Q000502:physiology*
chemical_list: D016209:Interleukin-8; D012367:RNA, Viral; D011971:Receptors, Immunologic; C495344:TLR3 protein, human; D051196:Toll-Like Receptor 3; C452820:RIGI protein, human; C452815:IFIH1 protein, human; D000071457:DEAD Box Protein 58; D053487:DEAD-box RNA Helicases; D000072640:Interferon-Induced Helicase, IFIH1; D011070:Poly I-C