title: Effect of trans8, cis10+cis9, trans11 conjugated linoleic acid mixture on lipid metabolism in 3T3-L1 cells.
abstract: Evidence suggests that minor isomers of conjugated linoleic acid (CLA), such as trans8, cis10 CLA, can elicit unique biological effects of their own. In order to determine the effect of a mixture of t8, c10+c9, t11 CLA isomers on selected aspects of lipid metabolism, 3T3-L1 preadipocytes were differentiated for 8 days in the presence of 100 microM linoleic acid (LA); t8, c10+c9, t11 CLA; t10, c12+c9, t11 CLA or purified c9, t11 CLA. Whereas supplementation with c9, t11 and t10, c12+c9, t11 CLA resulted in cellular triglyceride (TG) concentrations of 3.4 +/- 0.26 and 1.3 +/- 0.11 microg TG/microg protein, respectively (P < 0.05), TG accumulation following treatment with CLA mixture t8, c10+c9, t11 was significantly intermediate (2.5 +/- 0.22 microg TG/microg protein, P < 0.05) between the two other CLA treatments. However, these effects were not attributable to an alteration of the Delta(9) desaturation index. Adiponectin content of adipocytes treated with t8, c10+c9, t11 mixture was similar to the individual isomer c9, t11 CLA, and both the t8, c10+c9, t11 and c9, t11 CLA groups were greater (P < 0.05) than in the t10, c12+c9, t11 CLA group. Overall, these results suggest that t8, c10+c9, t11 CLA mixture affects TG accumulation in 3T3-L1 cells differently from the c9, t11 and t10, c12 isomers. Furthermore, the reductions in TG accumulation occur without adversely affecting the adiponectin content of these cells.
authors.affiliation: Department of Food Science and Nutrition, Institute of Nutraceuticals and Functional Foods, Laval University, 2425 Agriculture St., Paul-Comtois Building, Quebec, QC G1V 0A6, Canada.
authors.forename: Shama V
authors.initials: SV
authors.lastname: Joseph
authors.forename: Jessica R
authors.initials: JR
authors.lastname: Miller
authors.forename: Roger S
authors.initials: RS
authors.lastname: McLeod
authors.forename: Hélène
authors.initials: H
authors.lastname: Jacques
mesh_terms: D041721:3T3-L1 Cells; D017667:Adipocytes / Q000187:drug effects / Q000378:metabolism / Q000502:physiology; D050156:Adipogenesis / Q000187:drug effects; D052242:Adiponectin / Q000032:analysis / Q000378:metabolism; D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D044243:Linoleic Acids, Conjugated / Q000737:chemistry / Q000494:pharmacology*; D050356:Lipid Metabolism / Q000187:drug effects*; D051379:Mice; D014280:Triglycerides / Q000032:analysis / Q000378:metabolism
chemical_list: D052242:Adiponectin; C499919:Adipoq protein, mouse; D044243:Linoleic Acids, Conjugated; D014280:Triglycerides