title: [High throughput detection of drug-resistance gene mutations in HBV using MALDI-TOF mass spectrometry].
abstract: OBJECTIVE
  To develop a high-throughput clinical method on drug-resistance gene mutations of HBV using MALDI-TOF-MS.
  
  
  METHODS
  Using MassArray Assay Design software designed the iPLEX primers and followed the iPLEX instruction for amplification, SAP reaction, primer extenction, desalination, dispensing, MALDI-TOF-MS screening and data analysis of the gene mutation locus. 138 serum samples of chronic HBV patients with single drug-resistance or multiple drug-resistance on Lamivudin, adefovi, Entecavir were detected.
  
  
  RESULTS
  The HBV gene mutation platform was successfully developed and applied on the high-throughput dectection of clinical serum samples. It was also a high throughput assay which could be used to detect for more than 138 samples once. The MALDI-TOF-MS technology and the DNA sequencing simultaneously examine 33 samples, in which result of 10 sample is inconsistent, the including 2 samples by MALDI-TOF-MS technology has not tested, 1 sample has 2 inconsistent mutations.
  
  
  CONCLUSIONS
  Detection of HBV gene mutations using MALDI-TOF-MS is highly-sensitive, highly-accurate, high-throughput, fast achieved and suitable to use in the diagnosis and monitoring of HBV.
authors.affiliation: Shenzhen Donghu Hospital, Shenzhen 548020, China.
authors.forename: Bo-ping
authors.initials: BP
authors.lastname: Zhou
authors.forename: Hong-mei
authors.initials: HM
authors.lastname: Zhang
authors.forename: Xiao-he
authors.initials: XH
authors.lastname: Li
authors.forename: Jing
authors.initials: J
authors.lastname: Yuan
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.forename: Liu-mei
authors.initials: LM
authors.lastname: Xu
authors.forename: Huo-sheng
authors.initials: HS
authors.lastname: Wang
authors.forename: Xin-chun
authors.initials: XC
authors.lastname: Chen
authors.forename: Chun-ming
authors.initials: CM
authors.lastname: Ding
mesh_terms: D004279:DNA, Viral / Q000235:genetics; D004351:Drug Resistance / Q000235:genetics*; D024882:Drug Resistance, Viral / Q000187:drug effects* / Q000235:genetics*; D006515:Hepatitis B virus / Q000187:drug effects*; D013058:Mass Spectrometry; D020641:Polymorphism, Single Nucleotide
chemical_list: D004279:DNA, Viral