Plasma PAF-acetylhydrolase activity, inflammatory markers and susceptibility of LDL to in vitro oxidation in patients with type 1 diabetes mellitus.
OBJECTIVE
We evaluated if type 1 diabetic patients have altered levels of low-grade inflammatory markers including platelet-activating factor acetylhydrolase (PAF-AH) activity, high-sensitivity C-reactive protein (hs-CRP), white blood cells count (WBC), erythrocyte sedimentation rate (ESR) and LDL-cholesterol oxidation coefficient (OxC), and whether their levels are related to clinical complications and the metabolic profile.


METHODS
We studied cross-sectionally 118 outpatients with type 1 diabetes both with (n=38) and without (n=80) clinical chronic complications, and 110 controls subjects.


RESULTS
Diabetic patients had higher levels of PAF-AH activity (p=0.02), hs-CRP (p=0.001), WBC (p=0.004) and ESR (p=0.0001) than controls. Patients with type 1 diabetes had earlier OxC than the non-diabetic subjects at 1 and 3 h (p=0.01) after the addition of CuSO(4). No difference was noted between patients with and without chronic complications. In the patients, correlations were found between PAF-AH activity and OxC at 1 and 3 h.


CONCLUSIONS
It was concluded that the susceptibility of LDL to in vitro oxidation and low-grade inflammation are increased in patients with type 1 diabetes and related to each other. The presence of these markers is probably independent of the presence of chronic clinical complications and reveals the development of early pro-inflammatory state.
Gomes
Marilia Brito
MB
Department of Medicine, Diabetes Unit, State University of Rio de Janeiro, Brazil. mariliabgomes@uol.com.br
Cobas
Roberta Arnold
RA
Nunes
Edson
E
Castro-Faria-Neto
Hugo Caire
HC
da Matta
Maria Fátima Bevilacqua
MF
Neves
Ricardo
R
Tibiriçá
Eduardo
E
D043203:1-Alkyl-2-acetylglycerophosphocholine Esterase / Q000097:blood*; D000293:Adolescent; D000328:Adult; D015415:Biomarkers / Q000097:blood; D002097:C-Reactive Protein / Q000378:metabolism; D008078:Cholesterol, LDL / Q000097:blood; D003430:Cross-Sectional Studies; D003922:Diabetes Mellitus, Type 1 / Q000097:blood* / Q000201:enzymology*; D005260:Female; D006801:Humans; D007249:Inflammation / Q000097:blood*; D007958:Leukocyte Count; D008297:Male; D008875:Middle Aged; D010045:Outpatients; D010084:Oxidation-Reduction; D012016:Reference Values; D011795:Surveys and Questionnaires; D055815:Young Adult
D015415:Biomarkers; D008078:Cholesterol, LDL; D002097:C-Reactive Protein; D043203:1-Alkyl-2-acetylglycerophosphocholine Esterase