title: A novel beta-glucan produced by Paenibacillus polymyxa JB115 induces nitric oxide production in RAW264.7 macrophages.
abstract: The effect of extracellular beta-(1-->3), (1-->6)-glucan, produced by Paenibacillus polymyxa JB115, on nitric oxide (NO) production in RAW264.7 macrophages was investigated. beta-glucan induced the production of NO by RAW264.7 macrophages in a concentration- and time-dependent manner. Moreover, beta-glucan stimulation increased the mRNA expression of iNOS, COX-2 and IL-6 in RAW264.7 macrophages in a concentration-dependent manner.
authors.affiliation: College of Veterinary Medicine, Kyungpook National University, Daegu, Korea.
authors.forename: Zhi Qiang
authors.initials: ZQ
authors.lastname: Chang
authors.forename: Joong Su
authors.initials: JS
authors.lastname: Lee
authors.forename: Mi Hyun
authors.initials: MH
authors.lastname: Hwang
authors.forename: Joo Heon
authors.initials: JH
authors.lastname: Hong
authors.forename: Hee Kyoung
authors.initials: HK
authors.lastname: Jung
authors.forename: Sam Pin
authors.initials: SP
authors.lastname: Lee
authors.forename: Seung Chun
authors.initials: SC
authors.lastname: Park
mesh_terms: D000818:Animals; D001407:Bacillus / Q000378:metabolism*; D002460:Cell Line; D051546:Cyclooxygenase 2 / Q000096:biosynthesis / Q000235:genetics; D015850:Interleukin-6 / Q000096:biosynthesis / Q000235:genetics; D008070:Lipopolysaccharides / Q000494:pharmacology; D008264:Macrophages / Q000187:drug effects* / Q000201:enzymology / Q000276:immunology; D051379:Mice; D009569:Nitric Oxide / Q000096:biosynthesis* / Q000276:immunology; D052247:Nitric Oxide Synthase Type II / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D047071:beta-Glucans / Q000378:metabolism / Q000494:pharmacology*
chemical_list: D015850:Interleukin-6; D008070:Lipopolysaccharides; D012333:RNA, Messenger; D047071:beta-Glucans; D009569:Nitric Oxide; D052247:Nitric Oxide Synthase Type II; C077623:Ptgs2 protein, mouse; D051546:Cyclooxygenase 2