Deletion of methylglyoxal synthase gene (mgsA) increased sugar co-metabolism in ethanol-producing Escherichia coli.
The use of lignocellulose as a source of sugars for bioproducts requires the development of biocatalysts that maximize product yields by fermenting mixtures of hexose and pentose sugars to completion. In this study, we implicate mgsA encoding methylglyoxal synthase (and methylglyoxal) in the modulation of sugar metabolism. Deletion of this gene (strain LY168) resulted in the co-metabolism of glucose and xylose, and accelerated the metabolism of a 5-sugar mixture (mannose, glucose, arabinose, xylose and galactose) to ethanol.
Yomano
L P
LP
Department of Microbiology and Cell Science, University of Florida, Gainesville, FL 32611, USA.
York
S W
SW
Shanmugam
K T
KT
Ingram
L O
LO
D050260:Carbohydrate Metabolism; D019757:Carbon-Oxygen Lyases / Q000235:genetics*; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004926:Escherichia coli / Q000201:enzymology* / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000235:genetics*; D000431:Ethanol / Q000378:metabolism*; D017353:Gene Deletion; D008031:Lignin / Q000378:metabolism; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA
D004269:DNA, Bacterial; D029968:Escherichia coli Proteins; C036909:lignocellulose; D000431:Ethanol; D008031:Lignin; D019757:Carbon-Oxygen Lyases; C021578:methylglyoxal synthase