title: Adeno-associated virus infection of murine fibroblasts with help provided by mouse adenovirus.
abstract: Adeno-associated virus (AAV-2) replicates to high titers when host cells are coinfected with a helper virus. Here we analyzed the coinfection of AAV-2 and mouse adenovirus (MAV-1) in murine fibroblasts. We observed that AAV-2/MAV-1 coinfected NIH 3T3 cells produced approximately 10-40-fold less AAV-2 DNAse resistant particles than Hela cells. Levels of AAV-2 DNA replication were approximately 30-fold less in 3T3 cells as compared to Hela cells coinfected with human adenovirus (Ad-5). A study of these lower levels of infection in 3T3 cells compared to Hela cells revealed that receptor binding and internalization of AAV-2 in 3T3 and Hela cells was comparable. However, AAV-2 did not enter into the nucleus of mouse cells as efficiently as it does in human cells. Furthermore, viral DNA replication levels of AAV-2 DNA were found to be lower in mouse cells than human cells, indicating limitations in the murine nucleus for viral replication.
authors.affiliation: Department of Biochemistry and Cancer Biology, University of Toledo, College of Medicine, USA.
authors.forename: Vipul
authors.initials: V
authors.lastname: Bhrigu
authors.forename: James P
authors.initials: JP
authors.lastname: Trempe
mesh_terms: D000260:Adenoviruses, Human / Q000235:genetics / Q000502:physiology; D000818:Animals; D001483:Base Sequence; D002467:Cell Nucleus / Q000821:virology; D004261:DNA Replication; D004279:DNA, Viral / Q000235:genetics; D003850:Deoxyribonuclease I; D000229:Dependovirus / Q000235:genetics* / Q000502:physiology*; D006367:HeLa Cells; D006378:Helper Viruses / Q000235:genetics* / Q000502:physiology*; D006801:Humans; D017921:Mastadenovirus / Q000235:genetics* / Q000502:physiology*; D051379:Mice; D041681:NIH 3T3 Cells; D010957:Plasmids / Q000235:genetics; D011991:Receptors, Virus / Q000502:physiology; D013045:Species Specificity; D014162:Transfection; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism; D014779:Virus Replication
chemical_list: D004279:DNA, Viral; D011991:Receptors, Virus; D014764:Viral Proteins; D003850:Deoxyribonuclease I