title: Identification of novel genes involved in the plasticity of pituitary melanotropes in amphibians.
abstract: Melanotrope cells from the amphibian intermediate lobe are composed of two subpopulations that exhibit opposite secretory behavior: hypersecretory and hormone-storage hyposecretory melanotropes. Isolation of these subpopulations allowed a comparison of their gene expression profiles by differential display, leading to the identification of a number of genes differentially expressed in hypersecretory or hyposecretory melanotropes. Among them, we chose two (preferentially expressed in hyposecretory cells) of unknown function but structurally related to proteins involved in the secretory process: Rab18 and KIAA0555. We demonstrate that, upon activation of the regulated secretory pathway, Rab18 associates with secretory granules, inhibits their mobilization, and, consequently, reduces the secretory capacity of neuroendocrine cells. The other gene, KIAA0555, was predicted by in silico analysis to encode a protein with a long coiled-coil domain, a structural feature also shared by different proteins related to intracellular membrane traffic (i.e., golgins), and a hydrophobic C-terminal domain that could function as a transmembrane domain. A database search unveiled the existence of a KIAA0555 paralogue, KIAA4091, displaying a long coiled-coil region highly similar to that of KIAA0555 and an identical C-terminal transmembrane domain. Both KIAA0555 and KIAA4091 were found to be predominantly expressed in tissues containing cells with regulated secretory pathway, that is, endocrine and neural tissues. Moreover, when exogenously expressed in HEK293 cells, both proteins showed a yuxtanuclear distribution, which partially overlaps with that of a Golgi complex marker, thus suggesting a possible role of these two proteins in the control of the secretory process.
authors.affiliation: Department of Cell Biology, Physiology and Immunology, University of Córdoba, Córdoba, Spain. bc1mapom@uco.es
authors.forename: M M
authors.initials: MM
authors.lastname: Malagón
authors.forename: D
authors.initials: D
authors.lastname: Cruz-García
authors.forename: A
authors.initials: A
authors.lastname: Díaz-Ruiz
authors.forename: J R
authors.initials: JR
authors.lastname: Peinado
authors.forename: M R
authors.initials: MR
authors.lastname: Pulido
authors.forename: J
authors.initials: J
authors.lastname: Araújo
authors.forename: S
authors.initials: S
authors.lastname: Garcia-Navarro
authors.forename: F
authors.initials: F
authors.lastname: Gracia-Navarro
authors.forename: J P
authors.initials: JP
authors.lastname: Castaño
authors.forename: R
authors.initials: R
authors.lastname: Vázquez-Martínez
mesh_terms: D000663:Amphibians / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D005786:Gene Expression Regulation; D006801:Humans; D052717:Melanotrophs / Q000378:metabolism*; D020691:rab GTP-Binding Proteins / Q000737:chemistry / Q000378:metabolism
chemical_list: D020691:rab GTP-Binding Proteins