Changes in calbindin expression within the flocculus after unilateral labyrinthectomy in rats.
The role of flocculus in vestibular compensation is still a controversial issue. Calbindin regulates intracellular signaling and has been reported to be a reliable marker of Purkinje cell. Expression of calbindin in flocculus was examined using immunohistochemistry following unilateral labyrinthectomy (UL) in rats. Both the staining intensity and number of calbindin-positive Purkinje cells in the ipsilateral flocculus to the lesion side decreased 6h after UL compared to the control and contralateral side. Forty-eight hours after UL, the expression of calbindin returned to control levels and asymmetric expression in bilateral flocculus subsided. These transient reduction of calbindin expression in the ipsilateral flocculus may reflect a decrease in the GABAergic inhibition of the floccular Purkinje cell to the ipsilateral vestibular nuclei during vestibular compensation.
Hong
Seok Min
SM
Department of Otorhinolaryngology-Head and Neck Surgery, Chuncheon Sacred Heart Hospital, Hallym University College of Medicine, Chuncheon 200-704, Republic of Korea.
Cha
Chang Il
CI
Park
Byung Rim
BR
D000818:Animals; D064026:Calbindins; D002531:Cerebellum / Q000166:cytology / Q000378:metabolism*; D007839:Functional Laterality / Q000502:physiology*; D005786:Gene Expression Regulation / Q000502:physiology*; D008297:Male; D009474:Neurons / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D064030:S100 Calcium Binding Protein G / Q000378:metabolism*; D013997:Time Factors; D014722:Vestibule, Labyrinth / Q000601:surgery*
D064026:Calbindins; D064030:S100 Calcium Binding Protein G