title: DNA aneuploidy and dysplasia in oral potentially malignant disorders: association with cigarette smoking and site.
abstract: To date there are still no reliable biomarkers for oral potentially malignant disorders (PMDs) to predict the risk of progression to squamous cell carcinoma (SCC). Within a prospective clinical trial of patients with PMDs, DNA content flow cytometry (DNA FCM) was evaluated for 60 PMDs using fresh samples obtained by a dermatological curette. There were 6/42 PMDs without dysplasia, but with DNA aneuploidy, versus 8/18 with both dysplasia and aneuploidy (p=0.02). When the tongue and the buccal mucosa, the two most common sites in the present series of cases were compared, dysplastic PMDs were mainly located on the tongue (p=0.01). Tobacco smokers, who preferentially developed PMDs in the buccal mucosa at a younger age than non-smokers (p=0.002), had fewer dysplastic PMDs than did non-smokers (p=0.01). Dysplasia was significantly linked to DNA aneuploidy (p=0.03) in smokers. The present data suggest that aneuploidy is an early event in oral carcinogenesis and that the influence of tobacco varies according to subsite and patient age. When DNA FCM of PMD samples are obtained by curette scraping, extensive areas can be covered with a minimally invasive, rapid, inexpensive procedure. Moreover DNA FCM of these samples appears easy amenable to routine analysis. Further research on larger numbers of PMDs should be carried out to determine whether DNA FCM plays a role in the prediction of risk of PMD transformation.
authors.affiliation: Department of Clinical and Biological Sciences, Oral Medicine and Oral Oncology Section, University of Turin, Regione Gonzole 10, 10043 Orbassano (TO), Italy. monica.pentenero@unito.it
authors.forename: Monica
authors.initials: M
authors.lastname: Pentenero
authors.forename: Walter
authors.initials: W
authors.lastname: Giaretti
authors.forename: Roberto
authors.initials: R
authors.lastname: Navone
authors.forename: Anna
authors.initials: A
authors.lastname: Demurtas
authors.forename: Isabella
authors.initials: I
authors.lastname: Rostan
authors.forename: Giorgio
authors.initials: G
authors.lastname: Bertolusso
authors.forename: Roberto
authors.initials: R
authors.lastname: Broccoletti
authors.forename: Paolo Giacomo
authors.initials: PG
authors.lastname: Arduino
authors.forename: Davide
authors.initials: D
authors.lastname: Malacarne
authors.forename: Sergio
authors.initials: S
authors.lastname: Gandolfo
mesh_terms: D000367:Age Factors; D000368:Aged; D000782:Aneuploidy; D002294:Carcinoma, Squamous Cell / Q000235:genetics / Q000473:pathology*; D005260:Female; D005434:Flow Cytometry; D005819:Genetic Markers; D006801:Humans; D008297:Male; D008875:Middle Aged; D009061:Mouth Mucosa / Q000473:pathology; D009062:Mouth Neoplasms / Q000235:genetics / Q000473:pathology*; D011230:Precancerous Conditions / Q000235:genetics / Q000473:pathology*; D011446:Prospective Studies; D012907:Smoking / Q000009:adverse effects* / Q000235:genetics; D014059:Tongue / Q000473:pathology
chemical_list: D005819:Genetic Markers