title: Neuroinvasion in sheep transmissible spongiform encephalopathies: the role of the haematogenous route.
abstract: BACKGROUND
  It is generally believed that after oral exposure to transmissible spongiform encephalopathy (TSE) agents, neuroinvasion occurs via the enteric nervous system (ENS) and the autonomic nervous system. As a result, the dorsal motor nucleus of the vagus nerve is the initial point of disease-associated prion protein (PrP(d)) accumulation in the brain. HYPOTHESIS AND AIM: If direct ENS invasion following oral infection results in an early and specific brain targeting for PrP(d) accumulation, such topographical distribution could be different when other routes of infection were used, highlighting distinct routes for neuroinvasion.
  
  
  METHODS
  An immunohistochemical study has been conducted on the brain of 67 preclinically infected sheep exposed to natural scrapie or to experimental TSE infection by various routes.
  
  
  RESULTS
  Initial PrP(d) accumulation consistently occurred in the dorsal motor nucleus of the vagus nerve followed by the hypothalamus, regardless of the breed of sheep, PrP genotype, TSE source and, notably, route of infection; these factors did not appear to affect the topographical progression of PrP(d) deposition in the brain either. Moreover, the early and consistent appearance of PrP(d) aggregates in the circumventricular organs, where the blood-brain barrier is absent, suggests that these organs can provide a portal for entry of prions when infectivity is present in blood.
  
  
  CONCLUSIONS
  The haematogenous route, therefore, can represent a parallel or alternative pathway of neuroinvasion to ascending infection via the ENS/autonomic nervous system.
authors.affiliation: Department of Pathology, Veterinary Laboratories Agency, Bush Loan, Penicuik, Midlothian, UK. siso@vla.defra.gsi.gov.uk
authors.forename: S
authors.initials: S
authors.lastname: Sisó
authors.forename: M
authors.initials: M
authors.lastname: Jeffrey
authors.forename: L
authors.initials: L
authors.lastname: González
mesh_terms: D000818:Animals; D001812:Blood-Brain Barrier; D001921:Brain / Q000378:metabolism* / Q000473:pathology; D002552:Cerebral Ventricles / Q000378:metabolism; D018450:Disease Progression; D017615:Enteric Nervous System / Q000378:metabolism / Q000473:pathology; D005838:Genotype; D007031:Hypothalamus / Q000378:metabolism; D007150:Immunohistochemistry; D018620:PrPSc Proteins / Q000097:blood* / Q000378:metabolism; D017096:Prion Diseases / Q000378:metabolism* / Q000473:pathology / Q000635:transmission*; D011328:Prions / Q000097:blood* / Q000235:genetics / Q000378:metabolism*; D012608:Scrapie / Q000378:metabolism; D012756:Sheep; D013045:Species Specificity; D014630:Vagus Nerve / Q000378:metabolism
chemical_list: D018620:PrPSc Proteins; D011328:Prions