[Molecular cloning and characterization of Ras-homologues from different developing stages of Echinococcus granulosus].
The Ras GTPase gene from protoscolex and adult worm of Echinococcus granulosus in Xinjiang were cloned by RT-PCR and named as Eg Ras-pro (GenBank No. EU560397) and Eg Ras-adult (GenBank No. EU560398). Sequence analysis showed that each gene had 552 bp, coding 184 aa with an pI of 6.54. Bioinformatics analysis revealed that Eg Ras-pro and Eg Ras-adult had 98.4% and 98.9% homology to Echinococcus multilocularis Ras and 53.9%-78.8% homology to other species. Phylogenetic analysis showed that Eg Ras-pro and Eg Ras-adult clustered with EmRas and SmRas. The data indicated that EgRas GTPase has been expressed from protoscolex and adult worm of Echinococcus granulosus, and both are highly conservative.
Lv
Guo-dong
GD
Key Laboratory of Xinjiang Hydatid Institute, The First Teaching Hospital of Xinjiang Medical University, Urumqi 830054, China.
Wang
Jun-hua
JH
Lu
Xiao-mei
XM
Wen
Hao
H
Lin
Ren-yong
RY
D000818:Animals; D003001:Cloning, Molecular; D048209:Echinococcus granulosus / Q000201:enzymology / Q000235:genetics*; D015801:Helminth Proteins / Q000235:genetics*; D020559:Monomeric GTP-Binding Proteins / Q000235:genetics*; D010802:Phylogeny; D017422:Sequence Analysis, DNA
D015801:Helminth Proteins; D020559:Monomeric GTP-Binding Proteins