title: The up-regulation of microRNA-335 is associated with lipid metabolism in liver and white adipose tissue of genetically obese mice.
abstract: MicroRNAs (miRNAs) are short non-coding RNA that post-transcriptionally regulates gene expression. Some miRNAs have been proposed to be associated with obesity. However, miRNAs, which are related to the development of obesity in vivo remains unknown. Here in, we found the up-regulation of miR-335 in obesity using microarray analysis for miRNA. The expressions of miR-335 in liver and white adipose tissue (WAT) were up-regulated in obese mice including ob/ob, db/db, and KKAy mice. Increased miR-335 expressions were associated with an elevated body, liver and WAT weight, and hepatic triglyceride and cholesterol. Furthermore, miR-335 levels were closely correlated with expression levels of adipocyte differentiation markers such as PPARgamma, aP2, and FAS in 3T3-L1 adipocyte. These findings provide the first evidence that the up-regulated expressions of miR-335 in liver and WAT of obese mice might contribute to the pathophysiology of obesity.
authors.affiliation: Department of Internal Medicine (Endocrinology and Metabolism), Graduate School of Comprehensive Human Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8575, Japan.
authors.forename: Noriko
authors.initials: N
authors.lastname: Nakanishi
authors.forename: Yoshimi
authors.initials: Y
authors.lastname: Nakagawa
authors.forename: Naoko
authors.initials: N
authors.lastname: Tokushige
authors.forename: Naohito
authors.initials: N
authors.lastname: Aoki
authors.forename: Takashi
authors.initials: T
authors.lastname: Matsuzaka
authors.forename: Kiyoaki
authors.initials: K
authors.lastname: Ishii
authors.forename: Naoya
authors.initials: N
authors.lastname: Yahagi
authors.forename: Kazuto
authors.initials: K
authors.lastname: Kobayashi
authors.forename: Shigeru
authors.initials: S
authors.lastname: Yatoh
authors.forename: Akimitsu
authors.initials: A
authors.lastname: Takahashi
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Suzuki
authors.forename: Osamu
authors.initials: O
authors.lastname: Urayama
authors.forename: Nobuhiro
authors.initials: N
authors.lastname: Yamada
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Shimano
mesh_terms: D041721:3T3-L1 Cells; D050156:Adipogenesis / Q000235:genetics*; D052436:Adipose Tissue, White / Q000378:metabolism*; D000818:Animals; D050356:Lipid Metabolism / Q000235:genetics*; D008099:Liver / Q000378:metabolism*; D051379:Mice; D008820:Mice, Obese; D035683:MicroRNAs / Q000096:biosynthesis* / Q000235:genetics; D009765:Obesity / Q000235:genetics / Q000378:metabolism*; D020411:Oligonucleotide Array Sequence Analysis; D015854:Up-Regulation
chemical_list: D035683:MicroRNAs; C541986:Mirn335 microRNA, mouse