title: Development of a pUC19-based recombinant plasmid to serve as a positive control in PCR for Orientia tsutsugamushi.
abstract: A recombinant positive control plasmid was developed to be used in PCR for Orientia tsutsugamushi. This pUC19-based plasmid contains the O. tsutsugamushi DNA sequence, part of which is replaced with Candida albicans DNA. pUC19-Posi is a PCR control that provides several advantages over currently used positive controls for both universal and serotypical PCR.
authors.affiliation: Department of Virology-I, National Institute of Infectious Diseases, Shinjuku-ku, Tokyo 162-8640, Japan.
authors.forename: Nozomu
authors.initials: N
authors.lastname: Hanaoka
authors.forename: Akiko
authors.initials: A
authors.lastname: Sakata
authors.forename: Ai
authors.initials: A
authors.lastname: Takano
authors.forename: Hiroki
authors.initials: H
authors.lastname: Kawabata
authors.forename: Haruo
authors.initials: H
authors.lastname: Watanabe
authors.forename: Ichiro
authors.initials: I
authors.lastname: Kurane
authors.forename: Toshio
authors.initials: T
authors.lastname: Kishimoto
authors.forename: Shuji
authors.initials: S
authors.lastname: Ando
mesh_terms: D002176:Candida albicans / Q000235:genetics; D004274:DNA, Recombinant / Q000235:genetics*; D012285:Orientia tsutsugamushi / Q000235:genetics*; D010957:Plasmids / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods / Q000592:standards*; D012015:Reference Standards
chemical_list: D004274:DNA, Recombinant