Nondestructive quantum dot-based intracellular serotonin imaging in intact cells.
In recent years, quantum dots (Qdot), with their unique physical, chemical, and optical properties, have been used extensively as probes to visualize several cell membrane receptors and extracellular biomolecules. However, Qdot-based intracellular imaging has always been associated with vital lacunas. High affinity between quantum dots may induce serious aggregation in the cytoplasm; as a result, quantum dot aggregates are usually misinterpreted as quantum dot-probed intracellular molecules. Moreover, due to the more viscous nature of the cytoplasm versus the extracellular aqueous media, aggregation issues become more severe during intracellular studies. In this work, we suggest direct nondestructive serotonin imaging in an intact cell using the quantum dot-based immunoassay with a rapid tunable multicolor imaging system based on the acousto-optic tunable filter. Any false-positive intracellular serotonin molecules that appeared due to the aggregation of quantum dots could be completely discriminated from the real intracellular serotonin granules through multicolor cellular imaging. The developed method is quick and has wide applicability in targeting various intracellular proteins, coenzymes, and micronutrients.
Ki
Hyeon A
HA
Research Institute of Pharmaceutical Sciences and College of Pharmacy, Seoul National University, Seoul 151-742, Republic of Korea.
Naoghare
Pravin K
PK
Oh
Byung-Keun
BK
Choi
Jeong-Woo
JW
Song
Joon Myong
JM
D045744:Cell Line, Tumor; D005404:Fixatives / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry; D006801:Humans; D007118:Immunoassay; D008856:Microscopy, Fluorescence / Q000379:methods*; D045663:Quantum Dots; D012701:Serotonin / Q000737:chemistry*
D005404:Fixatives; D005456:Fluorescent Dyes; D012701:Serotonin