Assessment of feasibility of maillard reaction between baclofen and lactose by liquid chromatography and tandem mass spectrometry, application to pre formulation studies.
The aim of this study was to determine any possible, baclofen-lactose Maillard reaction products. Granules and tablets of baclofen and lactose were prepared and maintained in heat ovens for a certain time period. The effects of lactose type, addition of magnesium stearate, and water were monitored. Heated lactose and baclofen were analyzed using reverse-phase HPLC. Liquid chromatography tandem mass spectroscopy revealed nominal mass values consistent with baclofen-lactose, early-stage Maillard reaction condensation products (ESMRP). Multiple reaction monitoring confirmed the presence of ESMRP as well. FTIR analysis proved the formation of imine bond. The results indicated that baclofen undergoes a Maillard-type reaction with lactose.
Monajjemzadeh
Farnaz
F
Department of Pharmaceutics, Tabriz University of Medical Sciences, Tabriz, Iran.
Hassanzadeh
Davoud
D
Valizadeh
Hadi
H
Siahi-Shadbad
Mohammad R
MR
Mojarrad
Javid Shahbazi
JS
Robertson
Thomas
T
Roberts
Michael S
MS
D001418:Baclofen / Q000737:chemistry*; D002626:Chemistry, Pharmaceutical; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D007785:Lactose / Q000737:chemistry*; D015416:Maillard Reaction; D017550:Spectroscopy, Fourier Transform Infrared; D013607:Tablets / Q000737:chemistry; D053719:Tandem Mass Spectrometry / Q000379:methods*
D013607:Tablets; D001418:Baclofen; D007785:Lactose