Proliferation and multi-differentiation potentials of human mesenchymal stem cells on thermoresponsive PDMS surfaces grafted with PNIPAAm.
The thermo-responsivity of PNIPAAm [poly(N-isopropylcarylamide)]-grafted PDMS [poly(dimethylsiloxane)] surface is a property that could be feasibly used for detaching cells adhered on the surface. We used benzophenone-initiated photopolymerization to graft PNIPAAm on PDMS substrates to construct the PNIPAAm-grafted PDMS surface and this PDMS surface was highly thermo-responsive. hMSCs (human mesenchymal stem cells) were used to analyse the proliferation and multi-differentiation of stem cells on the PNIPAAm-grafted PDMS surface. The results showed that hMSCs could adhere on the PNIPAAm-grafted PDMS surface at 37 degrees C and form cell colonies, and then become fibroblastic. The proliferation potential of hMSCs on the PNIPAAm-grafted PDMS surface was not significantly different from that on a plate surface coated with gelatin. However, as it proved easier to detach cells from the surface, by changing temperature, a higher viability of detached cells could be obtained with the PNIPAAm-grafted PDMS surface, using a temperature shift, compared with a gelatin-coated surface, where cells are detached by treatment with trypsin. hMSCs on the PNIPAAm-grafted PDMS surface were induced into osteoblasts, adipocytes and neurocytes under osteogenic medium, adipogenic medium and neurogenic medium respectively. The PNIPAAm-grafted PDMS surface was favourable for osteogenesis of hMSCs, although the potentials of adipogenesis and neurogenesis of hMSCs on the PNIPAAm-grafted PDMS surface were similar to those on the plate surface coated with gelatin. The above results demonstrate that the PNIPAAm-grafted PDMS surface not only kept the potentials of proliferation and multi-differentiation of hMSCs, but also increased the viability of hMSCs.
Shi
Dongyan
D
The Institute of Cell Biology and Genetics, College of Biological Sciences, Zhejiang University, Zijingang Campus, Hangzhou, Zhejiang 310058, China.
Ma
Dan
D
Dong
Feiqing
F
Zong
Chen
C
Liu
Liyue
L
Shen
Dan
D
Yuan
Wenji
W
Tong
Xiangmin
X
Chen
Hengwu
H
Wang
Jinfu
J
D000178:Acrylamides / Q000737:chemistry* / Q000494:pharmacology; D000180:Acrylic Resins; D050156:Adipogenesis / Q000187:drug effects; D000293:Adolescent; D000328:Adult; D001577:Benzophenones / Q000737:chemistry / Q000494:pharmacology; D002448:Cell Adhesion / Q000187:drug effects; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D049109:Cell Proliferation; D002478:Cells, Cultured; D004129:Dimethylpolysiloxanes / Q000737:chemistry* / Q000494:pharmacology; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D005780:Gelatin / Q000737:chemistry / Q000494:pharmacology; D015870:Gene Expression / Q000187:drug effects; D006801:Humans; D007381:Intermediate Filament Proteins / Q000235:genetics; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D039902:Multipotent Stem Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000235:genetics; D064231:Nestin; D055495:Neurogenesis / Q000187:drug effects; D010751:Phosphopyruvate Hydratase / Q000235:genetics; D011108:Polymers / Q000737:chemistry* / Q000494:pharmacology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013499:Surface Properties; D013696:Temperature; D055815:Young Adult
D000178:Acrylamides; D000180:Acrylic Resins; D001577:Benzophenones; D004129:Dimethylpolysiloxanes; D007381:Intermediate Filament Proteins; C577484:NES protein, human; D009419:Nerve Tissue Proteins; D064231:Nestin; D011108:Polymers; C052970:poly-N-isopropylacrylamide; C013830:baysilon; C047723:benzophenone; D005780:Gelatin; D010751:Phosphopyruvate Hydratase