[AFLP analysis of genetic diversity of Prunella].
OBJECTIVE
To explore the variety of the genetic polymorphism of eight Prunella germplasm resources by AFLP analysis.


METHODS
The amplified fragment length polymorphism (AFLP) tags were applied to screen out 32 selective amplification primer pairs, the amplified bands as original matrix were analyzed with NTSYS-PC software for the similarity between the Prunella germplasm and the construction of genetic phylogenetic tree.


RESULTS
SDS extraction of genomic Prunella DNA showed a good quality, could meet the requirements of AFLP analysis. From 32 selective amplification primer pairs, 10 pairs with strong polymorphism, better band and higher resolution were used for the construction of the AFLP Prunella fingerprint, all eight Prunella germplasms were separated, they were divided into 3 categories.


CONCLUSIONS
Prunella germplasm resources are rich in genetic diversity, certain morphological characteristics and differences are associate with genotype.
Shen
Yufeng
Y
College of Agriculture and Biotechnology of Zhengjiang University, Hangzhou 310029, China.
Sun
Yiming
Y
Shen
Xiaoxia
X
Jiang
Jianming
J
Wang
Zhian
Z
Yu
Xuping
X
D054458:Amplified Fragment Length Polymorphism Analysis; D018744:DNA, Plant / Q000032:analysis; D014644:Genetic Variation; D011110:Polymorphism, Genetic; D031347:Prunella / Q000145:classification / Q000235:genetics*
D018744:DNA, Plant