title: Molecular dissection of Tomato leaf curl virus resistance in tomato line TY172 derived from Solanum peruvianum.
abstract: Tomato yellow leaf curl virus (TYLCV) is devastating to tomato (Solanum lycopersicum) crops and resistant cultivars are highly effective in controlling the disease. The breeding line TY172, originating from Solanum peruvianum, is highly resistant to TYLCV. To map quantitative trait loci (QTLs) controlling TYLCV resistance in TY172, appropriate segregating populations were analyzed using 69 polymorphic DNA markers spanning the entire tomato genome. Results show that TYLCV resistance in TY172 is controlled by a previously unknown major QTL, originating from the resistant line, and four additional minor QTLs. The major QTL, we term Ty-5, maps to chromosome 4 and accounts for 39.7-46.6% of the variation in symptom severity among segregating plants (LOD score 33-35). The minor QTLs, originated either from the resistant or susceptible parents, were mapped to chromosomes 1, 7, 9 and 11, and contributed 12% to the variation in symptom severity in addition to Ty-5.
authors.affiliation: Institute of Plant Sciences, Agricultural Research Organization, The Volcani Center, Bet Dagan, Israel.
authors.forename: Ilana
authors.initials: I
authors.lastname: Anbinder
authors.forename: Moshe
authors.initials: M
authors.lastname: Reuveni
authors.forename: Raviv
authors.initials: R
authors.lastname: Azari
authors.forename: Ilan
authors.initials: I
authors.lastname: Paran
authors.forename: Sahadia
authors.initials: S
authors.lastname: Nahon
authors.forename: Haviva
authors.initials: H
authors.lastname: Shlomo
authors.forename: Lea
authors.initials: L
authors.lastname: Chen
authors.forename: Moshe
authors.initials: M
authors.lastname: Lapidot
authors.forename: Ilan
authors.initials: I
authors.lastname: Levin
mesh_terms: D000483:Alleles; D053839:Begomovirus / Q000235:genetics*; D016009:Chi-Square Distribution; D032461:Chromosomes, Plant; D003433:Crosses, Genetic; D018744:DNA, Plant / Q000235:genetics / Q000302:isolation & purification; D005819:Genetic Markers; D005820:Genetic Testing; D018745:Genome, Plant; D055106:Genome-Wide Association Study; D005838:Genotype; D007113:Immunity, Innate / Q000235:genetics*; D008126:Lod Score; D018551:Solanum lycopersicum / Q000821:virology; D020161:Physical Chromosome Mapping; D010935:Plant Diseases / Q000821:virology*; D018515:Plant Leaves / Q000821:virology; D040641:Quantitative Trait Loci; D032322:Solanum / Q000235:genetics*
chemical_list: D018744:DNA, Plant; D005819:Genetic Markers