[Construction of plasmid expression vector for specific peptide of the rubella virus E1 gene].
OBJECTIVE
To construct a recombinant plasmid vector of the RV specific fragment for expressing the specific fragment of RV E1 protein.


METHODS
RNA of the RV attenuated live vaccine Wistar RA27/3 strain was extracted and reversely transcribed. The specific fragment of the E1 gene was amplified and the PCR products cloned in the vector pGEX-2T after purification. Positive clones were selected and identified by two-enzyme digestion and sequence analysis.


RESULTS
A 330 bp target fragment was successfully cloned, and the sequence of the recombinant plasmid was consistent with the original sequence.


CONCLUSIONS
Successful cloning of the RV El specific fragment and the construction of the recombinant plasmid have laid a foundation for further expressing the recombinant protein.
Cao
Jing
J
Department of Immunology, School of Preclinical Medicine, Nanjing Medical University, Nanjing, Jiangsu 210029, China.
Huang
Yu-Feng
YF
Gao
Jian
J
Wang
Hao-Yang
HY
Lu
Jin-Chun
JC
D001483:Base Sequence; D003001:Cloning, Molecular; D015870:Gene Expression; D005822:Genetic Vectors; D008969:Molecular Sequence Data; D010957:Plasmids; D012367:RNA, Viral; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012412:Rubella virus / Q000235:genetics* / Q000276:immunology; D014759:Viral Envelope Proteins / Q000235:genetics* / Q000276:immunology
D012367:RNA, Viral; D014759:Viral Envelope Proteins; C061826:E1 envelope protein, Rubella virus