title: The effect of EDTA instillations on the rate of development of encrustation and biofilms in Foley catheters.
abstract: The aim of this research was to examine whether a daily instillation of tetra sodium ethylenediaminetetraacetic acid (EDTA) solution could reduce the rate at which encrustation by crystalline Proteus mirabilis biofilms blocks urinary catheters. Sets of three bladder models were fitted with size 14 all-silicone catheters. Tetra sodium EDTA solution was instilled into the catheter following biofilm development. Catheters were examined by digital photography and scanning electron microscopy for evidence of encrustation. The results showed that the mean time to blockage of the control catheters was 45 h for saline, 57 h for water and 67 h for those exposed to daily instillations of the EDTA solution. Statistical analysis confirmed that the mean encrustation rate on the EDTA-treated catheters was significantly lower than on the control-treated devices (P = 0.047). This in vitro study indicates that EDTA may have beneficial effects in reducing the complication of catheter encrustation and blockage by crystalline biofilms.
authors.affiliation: School of Medicine, University of West Virginia, Morgantown, WV, USA. biofilms@hotmail.co.uk
authors.forename: Steven L
authors.initials: SL
authors.lastname: Percival
authors.forename: Nora A
authors.initials: NA
authors.lastname: Sabbuba
authors.forename: Peter
authors.initials: P
authors.lastname: Kite
authors.forename: David J
authors.initials: DJ
authors.lastname: Stickler
mesh_terms: D000818:Animals; D018441:Biofilms / Q000187:drug effects* / Q000254:growth & development*; D002408:Catheters, Indwelling / Q000382:microbiology*; D004492:Edetic Acid / Q000494:pharmacology*; D004866:Equipment Contamination; D006801:Humans; D008855:Microscopy, Electron, Scanning; D011513:Proteus mirabilis / Q000187:drug effects / Q000254:growth & development; D012828:Silicones; D012996:Solutions; D013997:Time Factors; D014546:Urinary Catheterization / Q000295:instrumentation*
chemical_list: D012828:Silicones; D012996:Solutions; D004492:Edetic Acid