title: Transcriptional dysregulation in NIPBL and cohesin mutant human cells.
abstract: Cohesin regulates sister chromatid cohesion during the mitotic cell cycle with Nipped-B-Like (NIPBL) facilitating its loading and unloading. In addition to this canonical role, cohesin has also been demonstrated to play a critical role in regulation of gene expression in nondividing cells. Heterozygous mutations in the cohesin regulator NIPBL or cohesin structural components SMC1A and SMC3 result in the multisystem developmental disorder Cornelia de Lange Syndrome (CdLS). Genome-wide assessment of transcription in 16 mutant cell lines from severely affected CdLS probands has identified a unique profile of dysregulated gene expression that was validated in an additional 101 samples and correlates with phenotypic severity. This profile could serve as a diagnostic and classification tool. Cohesin binding analysis demonstrates a preference for intergenic regions suggesting a cis-regulatory function mimicking that of a boundary/insulator interacting protein. However, the binding sites are enriched within the promoter regions of the dysregulated genes and are significantly decreased in CdLS proband, indicating an alternative role of cohesin as a transcription factor.
authors.affiliation: Division of Human Genetics, Abramson Research Institute, The Children's Hospital of Philadelphia, Philadelphia, Pennsylvania, United States of America.
authors.forename: Jinglan
authors.initials: J
authors.lastname: Liu
authors.forename: Zhe
authors.initials: Z
authors.lastname: Zhang
authors.forename: Masashige
authors.initials: M
authors.lastname: Bando
authors.forename: Takehiko
authors.initials: T
authors.lastname: Itoh
authors.forename: Matthew A
authors.initials: MA
authors.lastname: Deardorff
authors.forename: Dinah
authors.initials: D
authors.lastname: Clark
authors.forename: Maninder
authors.initials: M
authors.lastname: Kaur
authors.forename: Stephany
authors.initials: S
authors.lastname: Tandy
authors.forename: Tatsuro
authors.initials: T
authors.lastname: Kondoh
authors.forename: Eric
authors.initials: E
authors.lastname: Rappaport
authors.forename: Nancy B
authors.initials: NB
authors.lastname: Spinner
authors.forename: Hugo
authors.initials: H
authors.lastname: Vega
authors.forename: Laird G
authors.initials: LG
authors.lastname: Jackson
authors.forename: Katsuhiko
authors.initials: K
authors.lastname: Shirahige
authors.forename: Ian D
authors.initials: ID
authors.lastname: Krantz
mesh_terms: D018797:Cell Cycle Proteins / Q000235:genetics*; D011508:Chondroitin Sulfate Proteoglycans / Q000235:genetics; D047369:Chromatin Immunoprecipitation; D002868:Chromosomal Proteins, Non-Histone / Q000235:genetics*; D030541:Databases, Genetic; D003635:De Lange Syndrome / Q000235:genetics; D005786:Gene Expression Regulation; D020411:Oligonucleotide Array Sequence Analysis; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic / Q000235:genetics; D011506:Proteins / Q000235:genetics*; D000098550:Structural Maintenance of Chromosome Protein 1
chemical_list: D018797:Cell Cycle Proteins; D011508:Chondroitin Sulfate Proteoglycans; D002868:Chromosomal Proteins, Non-Histone; C487465:NIPBL protein, human; D011506:Proteins; C492318:SMC3 protein, human; D000098550:Structural Maintenance of Chromosome Protein 1