Corosolic acid induces apoptosis through mitochondrial pathway and caspase activation in human cervix adenocarcinoma HeLa cells.
We investigated the response of human cervix adenocarcinoma HeLa cells to Corosolic acid (CRA) treatment. Our results showed that CRA significantly inhibited cell viability in both a dose- and a time-dependent manner. CRA treatment induced S cell-cycle arrest and caused apoptotic death in HeLa cells. We found that CRA increased in Bax/Bcl-2 ratios by up-regulating Bax expression, disrupted mitochondrial membrane potential and triggered the release of cytochrome c from mitochondria into the cytoplasm. Moreover, CRA treatment triggered the activation of caspase-8, -9 and -3 in HeLa cells. All these results indicate that CRA-induced apoptosis is associated with the activation of caspases via a mitochondrial pathway. Taken together, we believe that CRA could have strong potentials for clinical application in treating human cervix adenocarcinoma and improving cancer chemotherapy.
Xu
Yanfeng
Y
Department of Traditional Chinese Medicine, Changhai Hospital, Second Military Medical University, Shanghai 200433, People's Republic of China.
Ge
Ruiliang
R
Du
Juan
J
Xin
Hailiang
H
Yi
Tingjiao
T
Sheng
Jiayu
J
Wang
Yongzi
Y
Ling
Changquan
C
D029042:Actinidia / Q000737:chemistry; D000230:Adenocarcinoma / Q000201:enzymology / Q000473:pathology*; D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D020169:Caspases / Q000502:physiology*; D004354:Drug Screening Assays, Antitumor; D004365:Drugs, Chinese Herbal / Q000737:chemistry; D004789:Enzyme Activation / Q000187:drug effects; D005260:Female; D006367:HeLa Cells; D006801:Humans; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D008928:Mitochondria / Q000187:drug effects* / Q000502:physiology; D015394:Molecular Structure; D009363:Neoplasm Proteins / Q000502:physiology*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000032:analysis; D016196:S Phase / Q000187:drug effects; D014315:Triterpenes / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D002583:Uterine Cervical Neoplasms / Q000201:enzymology / Q000473:pathology*; D051028:bcl-2-Associated X Protein / Q000032:analysis
D000972:Antineoplastic Agents, Phytogenic; C494826:BAX protein, human; D004365:Drugs, Chinese Herbal; D009363:Neoplasm Proteins; D019253:Proto-Oncogene Proteins c-bcl-2; D014315:Triterpenes; D051028:bcl-2-Associated X Protein; C113861:corosolic acid; D020169:Caspases