title: Characterization of olivetol synthase, a polyketide synthase putatively involved in cannabinoid biosynthetic pathway.
abstract: Alkylresorcinol moieties of cannabinoids are derived from olivetolic acid (OLA), a polyketide metabolite. However, the polyketide synthase (PKS) responsible for OLA biosynthesis has not been identified. In the present study, a cDNA encoding a novel PKS, olivetol synthase (OLS), was cloned from Cannabis sativa. Recombinant OLS did not produce OLA, but synthesized olivetol, the decarboxylated form of OLA, as the major reaction product. Interestingly, it was also confirmed that the crude enzyme extracts from flowers and rapidly expanding leaves, the cannabinoid-producing tissues of C. sativa, also exhibited olivetol-producing activity, suggesting that the native OLS is functionally expressed in these tissues. The possibility that OLS could be involved in OLA biosynthesis was discussed based on its catalytic properties and expression profile.
authors.affiliation: Graduate School of Pharmaceutical Sciences, Kyushu University, Fukuoka 812-8582, Japan. taura@phar.kyushu-u.ac.jp
authors.forename: Futoshi
authors.initials: F
authors.lastname: Taura
authors.forename: Shinji
authors.initials: S
authors.lastname: Tanaka
authors.forename: Chiho
authors.initials: C
authors.lastname: Taguchi
authors.forename: Tomohide
authors.initials: T
authors.lastname: Fukamizu
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Tanaka
authors.forename: Yukihiro
authors.initials: Y
authors.lastname: Shoyama
authors.forename: Satoshi
authors.initials: S
authors.lastname: Morimoto
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D002186:Cannabinoids / Q000096:biosynthesis*; D002188:Cannabis / Q000201:enzymology* / Q000235:genetics; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D018744:DNA, Plant / Q000235:genetics; D007700:Kinetics; D008969:Molecular Sequence Data; D048630:Polyketide Synthases / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D012118:Resorcinols / Q000378:metabolism*; D017386:Sequence Homology, Amino Acid; D013379:Substrate Specificity
chemical_list: D002186:Cannabinoids; D018076:DNA, Complementary; D018744:DNA, Plant; D011994:Recombinant Proteins; D012118:Resorcinols; C016630:olivetol; D048630:Polyketide Synthases