First detection of genotype 3 hepatitis E virus RNA in river water in Cambodia.
To identify and describe hepatitis E virus (HEV) strains in aquatic environments in Cambodia, we collected two river-water samples from upstream and downstream sites along the Siem Reap River and examined them for HEV using a virus concentration method followed by TaqMan reverse transcription PCR and genetic analysis of the PCR product. Genotype 3 HEV strain was detected from the river-water sample collected from the river downstream, indicating that genotype 3 HEV strains are circulating in this region. To our knowledge, this is the first report demonstrating HEV contamination in aquatic environments in Cambodia.
Kitajima
Masaaki
M
Laboratory of Public Health, Department of Urban Engineering, Graduate School of Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan. kitajima@env.t.u-tokyo.ac.jp
Matsubara
Koichi
K
Sour
Sethy
S
Haramoto
Eiji
E
Katayama
Hiroyuki
H
Ohgaki
Shinichiro
S
D002160:Cambodia; D004784:Environmental Monitoring; D005838:Genotype; D016752:Hepatitis E virus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D010802:Phylogeny; D012367:RNA, Viral / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D045483:Rivers / Q000821:virology*; D017422:Sequence Analysis, DNA; D014871:Water Microbiology
D012367:RNA, Viral