Enzyme encapsulation in microparticles composed of polymerized ionic liquids for highly active and reusable biocatalysts.
Horseradish peroxidase (HRP) is encapsulated in polymerized ionic liquid microparticles (pIL-MP), which are prepared by polymerization of 1-vinyl-3-ethylimidazolium bromide in the presence of the crosslinker N,N'-methylenebis(acrylamide) in a concentrated water-in-oil (W/O) emulsion. pIL-MP encapsulating HRP chemically-modified with comb-shaped polyethylene glycol (PM(13)-HRP) exhibit excellent activity for guaiacol oxidation in an aqueous solution. The PM(13)-HRP in pIL-MP shows more than 2-fold higher activity than that of the enzyme encapsulated in a polyacrylamide microparticle. The catalytic activity declines with an increase in the crosslinker concentration of the pIL-MP, probably due to suppression of substrate diffusion. The activity of PM(13)-HRP in pIL-MP depends on the external environment of the gel (i.e. pH and temperature). The pIL-MP are easily recovered from the reaction mixture by centrifugation, which makes it possible to recycle the biocatalyst for repeated oxidation reactions.
Nakashima
Kazunori
K
Department of Applied Chemistry, Graduate School of Engineering, Fukuoka 819-0395, Japan.
Kamiya
Noriho
N
Koda
Daisuke
D
Maruyama
Tatsuo
T
Goto
Masahiro
M
D000178:Acrylamides; D003432:Cross-Linking Reagents; D004655:Emulsions / Q000737:chemistry*; D004800:Enzymes, Immobilized / Q000737:chemistry* / Q000378:metabolism*; D006735:Horseradish Peroxidase / Q000737:chemistry* / Q000378:metabolism*; D052578:Ionic Liquids / Q000737:chemistry*; D010084:Oxidation-Reduction; D011145:Polyvinyls / Q000737:chemistry
D000178:Acrylamides; D003432:Cross-Linking Reagents; D004655:Emulsions; D004800:Enzymes, Immobilized; D052578:Ionic Liquids; D011145:Polyvinyls; D006735:Horseradish Peroxidase; C021221:N,N'-methylenebisacrylamide