Next-generation sequencing of vertebrate experimental organisms.
Next-generation sequencing technologies are revolutionizing biology by allowing for genome-wide transcription factor binding-site profiling, transcriptome sequencing, and more recently, whole-genome resequencing. While it is currently not possible to generate complete de novo assemblies of higher-vertebrate genomes using next-generation sequencing, improvements in sequence read lengths and throughput, coupled with new assembly algorithms for large data sets, will soon make this a reality. These developments will in turn spawn a revolution in how genomic data are used to understand genetics and how model organisms are used for disease gene discovery. This review provides an overview of the current next-generation sequencing platforms and the newest computational tools for the analysis of next-generation sequencing data. We also describe how next-generation sequencing may be applied in the context of vertebrate model organism genetics.
Turner
Daniel J
DJ
Experimental Cancer Genetics, Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Cambridge, UK.
Keane
Thomas M
TM
Sudbery
Ian
I
Adams
David J
DJ
D000818:Animals; D023281:Genomics; D006801:Humans; D017422:Sequence Analysis, DNA / Q000379:methods*; D014714:Vertebrates / Q000235:genetics*