Identification of a second messenger of crustacean hyperglycemic hormone signaling pathway in the kuruma prawn Marsupenaeus japonicus.
To address the signaling pathway of crustacean hyperglycemic hormone (CHH) in the kuruma prawn Marsupenaeus japonicus, we measured the intracellular cAMP and cGMP levels in the hepatopancreas, one of the major target tissues of CHH. Removal of CHH-producing cells by bilateral eyestalk ablation decreased intracellular cGMP levels, while little effect on cAMP was observed in the tissue. Similarly, exposure to recombinant CHH significantly increased the cGMP level of the hepatopancreas in vitro, while little change was observed in cAMP. These results suggest that cGMP, rather than cAMP, mediates the CHH signal in hepatopancreas of M. japonicus.
Nagai
Chiaki
C
Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan.
Asazuma
Hideaki
H
Nagata
Shinji
S
Nagasawa
Hiromichi
H
D000818:Animals; D060829:Arthropod Proteins; D000242:Cyclic AMP / Q000378:metabolism; D006152:Cyclic GMP / Q000378:metabolism; D007447:Invertebrate Hormones; D008099:Liver / Q000187:drug effects; D009419:Nerve Tissue Proteins / Q000378:metabolism* / Q000494:pharmacology; D010179:Pancreas / Q000187:drug effects; D033561:Penaeidae / Q000187:drug effects / Q000378:metabolism*; D015290:Second Messenger Systems / Q000187:drug effects; D015398:Signal Transduction / Q000187:drug effects
D060829:Arthropod Proteins; D007447:Invertebrate Hormones; D009419:Nerve Tissue Proteins; C015947:hyperglycemic hormone, crustacean; D000242:Cyclic AMP; D006152:Cyclic GMP