title: Biodegradation of brominated aromatics by cultures and laccase of Trametes versicolor.
abstract: 2-Bromophenol (1), 4-bromophenol (2), 2,4-dibromophenol (3), 2,6-dibromophenol (4), 2,4,6-tribromophenol (5) and tetrabromobisphenol A (6) (1 mM each) added to growing submerged cultures of Trametes versicolor CCBAS 612 were eliminated by 65-85% from the culture medium within 4d. Extracellular laccase activity in the culture medium was influenced by the type of brominated compound added. Maximum level of laccase (63 U L(-1)) was found in the culture with 2-bromophenol. Tetrabromobisphenol A was degraded by a commercial laccase from Trametes versicolor in absence of any oxidation mediator, hydroxylated dibrominated compounds being detected as soluble reaction products by LC/MS. A significant degradation of brominated phenols by laccase was achieved only in the presence of ABTS structural characterization of major products suggesting reaction between bromophenol and ABTS radicals.
authors.affiliation: Institute of Microbiology, Academy of Sciences, 142 20 Prague, Czech Republic. brona@biomed.cas.cz
authors.forename: B
authors.initials: B
authors.lastname: Uhnáková
authors.forename: A
authors.initials: A
authors.lastname: Petrícková
authors.forename: D
authors.initials: D
authors.lastname: Biedermann
authors.forename: L
authors.initials: L
authors.lastname: Homolka
authors.forename: V
authors.initials: V
authors.lastname: Vejvoda
authors.forename: P
authors.initials: P
authors.lastname: Bednár
authors.forename: B
authors.initials: B
authors.lastname: Papousková
authors.forename: M
authors.initials: M
authors.lastname: Sulc
authors.forename: L
authors.initials: L
authors.lastname: Martínková
mesh_terms: D001673:Biodegradation, Environmental; D006842:Hydrocarbons, Brominated / Q000737:chemistry / Q000378:metabolism*; D042845:Laccase / Q000378:metabolism*; D010636:Phenols / Q000737:chemistry / Q000378:metabolism*; D011075:Polybrominated Biphenyls / Q000378:metabolism; D055454:Trametes / Q000201:enzymology*; D014874:Water Pollutants, Chemical / Q000737:chemistry / Q000378:metabolism*
chemical_list: D006842:Hydrocarbons, Brominated; D010636:Phenols; D011075:Polybrominated Biphenyls; D014874:Water Pollutants, Chemical; C038964:2,6-dibromophenol; C032038:2-bromophenol; D042845:Laccase; C020806:tetrabromobisphenol A; C013930:2,4-dibromophenol; C032037:4-bromophenol; C004554:2,4,6-tribromophenol