Violet ZnSe/ZnS as an alternative to green CdSe/ZnS in nanocrystal-fluorescent protein FRET systems.
Fluorescent proteins from the green fluorescent protein family strongly interact with CdSe/ZnS and ZnSe/ZnS nanocrystals at neutral pH. Green emitting CdSe/ZnS nanocrystals and red emitting fluorescent protein dTomato constitute a 72% efficiency FRET system with the largest alteration of the overall photoluminescence profile, following complex formation, observed so far. The substitution of ZnSe/ZnS for CdSe/ZnS nanocrystals as energy donors enabled the use of a green fluorescent protein, GFP5, as energy acceptor. Violet emitting ZnSe/ZnS nanocrystals and green GFP5 constitute a system with 43% FRET efficiency and an unusually strong sensitized emission. ZnSe/ZnS-GFP5 provides a cadmium-free, high-contrast FRET system that covers only the high-energy part of the visible spectrum, leaving room for simultaneous use of the yellow and red color channels. Anisotropic fluorescence measurements confirmed the depolarization of GFP5 sensitized emission.
Hering
Vitor R
VR
Department of Biochemistry, Chemistry Institute, University of Sao Paulo, Sao Paulo, SP 05513-970, Brazil. vrhering@uol.com.br
Faulin
Tanize E S
TE
Triboni
Eduardo R
ER
Rodriguez
Silvio D
SD
Bernik
Delia L
DL
Schumacher
Robert I
RI
Mammana
Victor P
VP
Faljoni-Alario
Adelaide
A
Abdalla
Dulcineia S P
DS
Gibson
Gary
G
Politi
Mário J
MJ
D003116:Color; D005454:Fluorescence Polarization; D031541:Fluorescence Resonance Energy Transfer; D008164:Luminescent Proteins / Q000737:chemistry* / Q000378:metabolism; D053758:Nanoparticles / Q000737:chemistry*; D018036:Selenium Compounds / Q000737:chemistry* / Q000378:metabolism; D017967:Zinc Compounds / Q000737:chemistry* / Q000378:metabolism; D019287:Zinc Sulfate / Q000737:chemistry* / Q000378:metabolism
D008164:Luminescent Proteins; D018036:Selenium Compounds; D017967:Zinc Compounds; D019287:Zinc Sulfate; C044696:zinc selenide