[Isolation, identification and rumen fermentation characteristics of Propionibacterium acnes].
OBJECTIVE
Characteristic of energy metabolism in ruminant is a negative energy balance in perinatal period. Propionic acid from ruminal microbe fermentation is a vital glyconeogenesis substrate for preventing negative energy balance. We isolated and screened a Propionibacterium acnes strain from health cow rumen fluid, and studied its rumen fermentation characteristics.


METHODS
A Propionibacterium acnes strain from rumen fluid of health cow with permanent rumen fistula under sterile condition was isolated by segregation procedure of anaerobic bacterium and Sodium Lactate Broth (SLB), and identified by extraction of the genome DNA, cloning of the 16S rRNA gene, and sequencing. We studied the effect of the strain on pH, volatile fatty acid and lactic acid in rumen fluid in vitro and in vivo.


RESULTS
A bacterium isolated from health cow rumen fluid was identified as Propionibacterium acnes by morphology, biochemical characteristics and sequence homology. In vitro, pH in rumen fluid decreased to the lowest after rumen fermentation of the strain for 12 h, then increased gradually. However, concentration of volatile fatty acid, such as acetic acid, propionic acid and butyric acid, increased to the highest after rumen fermentation of the strain for 12 h, then decreased gradually in vitro. The concentration of lactic acid and ratio of acetate to propionate decreased overall in vitro. In vivo, pH in rumen fluid decreased overall, concentration, of the volatile fatty acid increased overall.


CONCLUSIONS
A strain of Propionibacterium acnes was isolated successfully from health cow rumen fluid. It is an important basis to develop microecological preparation for preventing cows' negative energy balance in perinatal period in future.
Wu
Ling
L
College of Animal Science and Technology, Heilongjiang Aug. 1st Land Reclamation University, Daqing 163319, China. wuling8@163.com
Zhao
Mingjuan
M
Xia
Cheng
C
Ni
Hongbo
H
Zhang
Hongyou
H
D000818:Animals; D002417:Cattle; D005232:Fatty Acids, Volatile / Q000378:metabolism; D005285:Fermentation; D011422:Propionates / Q000378:metabolism; D011425:Propionibacterium acnes / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*; D012417:Rumen / Q000382:microbiology*
D005232:Fatty Acids, Volatile; D011422:Propionates; C029658:propionic acid