Saccharomyces cerevisiae as a vaccine against coccidioidomycosis.
Disseminated coccidioidomycosis is a life-threatening infection. In these studies, we examined protection against systemic murine coccidioidomycosis by vaccination with heat-killed Saccharomyces cerevisiae (HKY). CD-1 mice received HKY subcutaneously or by oral gavage with or without adjuvants once weekly beginning 3 or 4 weeks prior to infection; oral live Saccharomyces was also studied. All HKY sc regimens were equivalent, prolonging survival (P<or=0.005) and reducing fungal burden versus controls. Oral live Saccharomyces, but not HKY, prolonged survival (P=0.03), but did not reduce fungal burden. Survival of mice given HKY was equivalent to vaccination with formalin-killed spherules, but inferior in reduction of fungal burden. HKY was superior to a successful recombinant vaccine, PRA plus adjuvant. This novel heterologous protection afforded by HKY vaccination offers a new approach to a vaccine against coccidioidomycosis.
Capilla
Javier
J
California Inst. for Med. Res., San Jose, CA, United States.
Clemons
Karl V
KV
Liu
Min
M
Levine
Hillel B
HB
Stevens
David A
DA
D000818:Animals; D003047:Coccidioidomycosis / Q000517:prevention & control*; D005657:Fungal Vaccines / Q000276:immunology*; D006801:Humans; D008297:Male; D051379:Mice; D012441:Saccharomyces cerevisiae / Q000276:immunology*; D014611:Vaccination
D005657:Fungal Vaccines