title: Combined dissolved oxygen and pH control strategy to improve the fermentative production of L-isoleucine by Brevibacterium lactofermentum.
abstract: The effect of both dissolved oxygen (DO) and pH on L: -isoleucine production by batch culture of Brevibacterium lactofermentum was investigated. A two-stage agitation speed control strategy was developed, and the isoleucine production reached 23.3 g L(-1) in a relative short time (52 h), increased by 11.6% compared to the results obtained in the single agitation speed control process. In order to make sure whether the combination of DO and pH control can boost the production by a mutual effect, different control modes were conducted, based on the data obtained from the two-stage agitation speed control strategy and the analysis of kinetics parameters at different pH values. The results showed that the mode of combining two-stage DO with two-stage pH control strategy was the optimal for isoleucine production. The isoleucine production can reach 26.6 g L(-1) at 56 h, increased by 14.3% comparing to that obtained by the single two-stage DO control strategy.
authors.affiliation: School of Biotechnology, Jiangnan University, 214122 Wuxi, China. zhijian2870@126.com
authors.forename: Zhijian
authors.initials: Z
authors.lastname: Peng
authors.forename: Jun
authors.initials: J
authors.lastname: Fang
authors.forename: Jianghua
authors.initials: J
authors.lastname: Li
authors.forename: Long
authors.initials: L
authors.lastname: Liu
authors.forename: Guocheng
authors.initials: G
authors.lastname: Du
authors.forename: Jian
authors.initials: J
authors.lastname: Chen
authors.forename: Xiaoyuan
authors.initials: X
authors.lastname: Wang
authors.forename: Jianfei
authors.initials: J
authors.lastname: Ning
authors.forename: Liming
authors.initials: L
authors.lastname: Cai
mesh_terms: D019149:Bioreactors / Q000382:microbiology*; D001709:Biotechnology / Q000379:methods*; D001951:Brevibacterium / Q000378:metabolism*; D005285:Fermentation; D005947:Glucose / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D007218:Industrial Microbiology / Q000379:methods; D007532:Isoleucine / Q000096:biosynthesis / Q000737:chemistry*; D007700:Kinetics; D010100:Oxygen / Q000737:chemistry*; D013997:Time Factors
chemical_list: D007532:Isoleucine; D005947:Glucose; D010100:Oxygen