title: Effect of cyclooxygenase-2 on the regulation of cerebral blood flow during neuronal activation in the rat.
abstract: The present study was designed to clarify the precision of the main approach for investigating the regulation of local cerebral blood flow (CBF) response to neuronal activation in the brain (neurovascular coupling). In this study, we examined the effects of NS-398, a highly selective cyclooxygenase-2 inhibitor, on the physiological variables, baseline CBF, and local CBF response during rat somatosensory neuronal activation by laser-Doppler flowmetry. Blood pressure and heart rate were significantly decreased 3h after i.v. infusion of NS-398. Baseline CBF and local CBF during somatosensory activation gradually decreased with an increase in time of NS-398 infusion up to 3h, although neuronal activity in the somatosensory area was almost constant during the infusion. The results suggest that cyclooxygenase-2 participates in the regulation of local CBF during neuronal activation in rats. The present study also revealed the potential side-effects of dimethylsulfoxide, a solvent of NS-398, on neurovascular coupling.
authors.affiliation: Academic Group of Mathematical and Natural Science, Iwate University, 4-3-5 Ueda, Morioka 020-8551, Japan. matsuura@iwate-u.ac.jp
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Matsuura
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Takuwa
authors.forename: Rumiana
authors.initials: R
authors.lastname: Bakalova
authors.forename: Takayuki
authors.initials: T
authors.lastname: Obata
authors.forename: Iwao
authors.initials: I
authors.lastname: Kanno
mesh_terms: D000818:Animals; D001921:Brain / Q000098:blood supply* / Q000187:drug effects; D002560:Cerebrovascular Circulation / Q000187:drug effects / Q000502:physiology*; D051546:Cyclooxygenase 2 / Q000502:physiology*; D052246:Cyclooxygenase 2 Inhibitors / Q000494:pharmacology; D005073:Evoked Potentials, Somatosensory / Q000187:drug effects; D008297:Male; D009431:Neural Conduction / Q000187:drug effects; D009578:Nitrobenzenes / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D013449:Sulfonamides / Q000494:pharmacology
chemical_list: D052246:Cyclooxygenase 2 Inhibitors; D009578:Nitrobenzenes; D013449:Sulfonamides; C080955:N-(2-cyclohexyloxy-4-nitrophenyl)methanesulfonamide; D051546:Cyclooxygenase 2; C489691:Ptgs2 protein, rat