title: Low dietary inorganic phosphate affects the lung growth of developing mice.
abstract: Inorganic phosphate (Pi) plays a critical role in diverse cellular functions, and regulating the Pi balance is accomplished by sodium-dependent Pi co-transporter (NPT). Pulmonary NPT has recently been identified in mammalian lungs. However, to date, many of the studies that have involved Pi have mainly focused on its effect on bone and kidney. Therefore, current study was performed to discover the potential effects of low Pi on the lung of developing transgenic mice expressing the renilla/firefly luciferase dual reporter gene. Two-weeks old male mice divided into 2 groups and these groups were fed either a low PI diet or a normal control diet (normal: 0.5% Pi, low: 0.1% Pi) for 4 weeks. After 4 weeks of the diet, all the mice were sacrificed. Their lungs were harvested and analyzed by performing luciferase assay, Western blotting, kinase assay and immunohistochemistry. Our results demonstrate that low Pi affects the lungs of developing mice by disturbing protein translation, the cell cycle and the expression of fibroblast growth factor-2. These results suggest that optimally regulating Pi consumption may be important to maintain health.
authors.affiliation: Laboratory of Toxicology, College of Veterinary Medicine, Seoul National University, Seoul, Korea.
authors.forename: Cheng Xiong
authors.initials: CX
authors.lastname: Xu
authors.forename: Hua
authors.initials: H
authors.lastname: Jin
authors.forename: Youn Sun
authors.initials: YS
authors.lastname: Chung
authors.forename: Ji Young
authors.initials: JY
authors.lastname: Shin
authors.forename: Soon Kyung
authors.initials: SK
authors.lastname: Hwang
authors.forename: Jung Taek
authors.initials: JT
authors.lastname: Kwon
authors.forename: Sung Jin
authors.initials: SJ
authors.lastname: Park
authors.forename: Eun Sun
authors.initials: ES
authors.lastname: Lee
authors.forename: Arash
authors.initials: A
authors.lastname: Minai-Tehrani
authors.forename: Seung Hee
authors.initials: SH
authors.lastname: Chang
authors.forename: Min Ah
authors.initials: MA
authors.lastname: Woo
authors.forename: Mi Suk
authors.initials: MS
authors.lastname: Noh
authors.forename: Gil Hwan
authors.initials: GH
authors.lastname: An
authors.forename: Kee Ho
authors.initials: KH
authors.lastname: Lee
authors.forename: Myung Haing
authors.initials: MH
authors.lastname: Cho
mesh_terms: D048868:Adaptor Proteins, Signal Transducing; D000818:Animals; D015153:Blotting, Western; D002352:Carrier Proteins / Q000378:metabolism; D018797:Cell Cycle Proteins; D039642:Eukaryotic Initiation Factors; D007150:Immunohistochemistry; D008168:Lung / Q000187:drug effects / Q000201:enzymology / Q000254:growth & development* / Q000378:metabolism; D008297:Male; D051379:Mice; D008822:Mice, Transgenic; D010750:Phosphoproteins / Q000378:metabolism; D016226:Phosphorus, Dietary / Q000008:administration & dosage*; D010766:Phosphorylation; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000378:metabolism; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D050613:Sodium-Phosphate Cotransporter Proteins, Type IIa / Q000378:metabolism*; D058570:TOR Serine-Threonine Kinases
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D002352:Carrier Proteins; D018797:Cell Cycle Proteins; C499527:Eif4ebp1 protein, mouse; D039642:Eukaryotic Initiation Factors; D010750:Phosphoproteins; D016226:Phosphorus, Dietary; D050613:Sodium-Phosphate Cotransporter Proteins, Type IIa; D017853:Phosphotransferases (Alcohol Group Acceptor); C546843:mTOR protein, mouse; D051057:Proto-Oncogene Proteins c-akt; D058570:TOR Serine-Threonine Kinases