Role of nsdA in negative regulation of antibiotic production and morphological differentiation in Streptomyces bingchengensis.
To investigate the function of nsdA in Streptomyces bingchengensis, it was cloned and sequenced, which presented an 89.89% identity with that of S. coelicolor. The lambdaRED-mediated PCR-targeting technique was used to create nsdA replacement in the S. bingchengensis_226541 chromosome. The nsdA disruption mutant, BC29, was obtained, which produced more pigment and spores than did the ancestral strain. HPLC analysis revealed that the disruption of nsdA efficiently increased milbemycin A(4) production and nanchangmycin production by 1.5-fold and 9-fold, respectively. Complementation of the nsdA mutation restored the phenotype and antibiotic production. These results showed that nsdA negatively affected sporulation and antibiotic production in S. bingchengensis.
Wang
Xiang-Jing
XJ
School of Life Science, Northeast Agricultural University, Harbin, China.
Guo
Suo-Lian
SL
Guo
Wan-Qian
WQ
Xi
Di
D
Xiang
Wen-Sheng
WS
D000900:Anti-Bacterial Agents / Q000096:biosynthesis*; D003001:Cloning, Molecular; D017931:DNA Primers; D004269:DNA, Bacterial / Q000032:analysis / Q000096:biosynthesis / Q000235:genetics; D005798:Genes, Bacterial / Q000235:genetics* / Q000502:physiology; D005822:Genetic Vectors; D010641:Phenotype; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013171:Spores, Bacterial / Q000235:genetics; D013302:Streptomyces / Q000254:growth & development / Q000378:metabolism* / Q000648:ultrastructure*
D000900:Anti-Bacterial Agents; D017931:DNA Primers; D004269:DNA, Bacterial