[HBV transinfected enhances the PD-L expression of cytokines stimulating].
OBJECTIVE
To investigate whether the PD-L expression in the liver cell lines transinfected with HBV (HepG2.2.15 cells) can be up-regulated after cytokines stimulating.


METHODS
To apply the liver cell lines (HepG2 cells and HepG2.2.15 cells) as a model, the cells were stimulated with IL-4, IFN-alpha and IFN-gamma (final concentration were 10 ng/ml, stimulated for 12 hours) and RT-PCR was carried out to determine the PD-L expression before and after cytokines stimulating.


RESULTS
Whether or not transinfected with HBV, IFN-alpha and IFN-gamma both can induce the liver cell lines (HepG2 cells and HepG2.2.15 cells) PD-L1 expression while IL-4 can not; IL-4, IFN-alpha, IFN-gamma all can induce the PD-L2 expression in HepG2.2.15 cells which was transinfected with HBV, only IFN-gamma can induce the PD-L2 expression in HepG2 cells which was not transinfected with HBV.


CONCLUSIONS
IFN-alpha, IFN-gamma both can induce the PD-L1 expression in HepG2 cells and HepG2.2.15 cells, while it is easy for cytokines to induce the PD-L2 expression in HepG2.2.15 cells which was transinfected with HBV, this may provide a potential mechanism of the molecular basis for chronic HBV infection.
Wu
Xue-jie
XJ
Department of Infectious Diseases, Peking University First Hospital, Beijing 100034, China.
Chen
Ji
J
Zhu
Man-hua
MH
Hong
Yuan
Y
Zhong
Lu
L
Wang
Gui-qiang
GQ
D045744:Cell Line, Tumor; D016207:Cytokines / Q000235:genetics / Q000378:metabolism*; D015870:Gene Expression; D006509:Hepatitis B / Q000378:metabolism* / Q000473:pathology; D006515:Hepatitis B virus; D022781:Hepatocytes / Q000378:metabolism* / Q000821:virology; D006801:Humans; D008099:Liver / Q000473:pathology*
D016207:Cytokines