title: Acute, food-induced moderate elevation of plasma uric acid protects against hyperoxia-induced oxidative stress and increase in arterial stiffness in healthy humans.
abstract: We examined the effects of acute, food-induced moderate increase of plasma uric acid (UA) on arterial stiffness and markers of oxidative damage in plasma in healthy males exposed to 100% normobaric oxygen. Acute elevation of plasma UA was induced by consumption of red wine, combination of ethanol and glycerol, or fructose. By using these beverages we were able to separate the effects of UA, wine polyphenols and ethanol. Water was used as a control beverage. Ten males randomly consumed test beverages in a cross-over design over the period of 4 weeks, one beverage per week. They breathed 100% O(2) between 60(th) and 90(th)min of the 4-h study protocol. Pulse wave augmentation index (AIx) at brachial and radial arteries, plasma antioxidant capacity (AOC), thiobarbituric acid-reactive substances (TBARS), lipid hydroperoxides (LOOH) assessed by xylenol orange method, UA and blood ethanol concentrations were determined before and 60, 90, 120, 150 and 240 min after beverage consumption. Consumption of the beverages did not affect the AIx, TBARS or LOOH values during 60 min before exposure to hyperoxia, while AOC and plasma UA increased except in the water group. Significant increase of AIx, plasma TBARS and LOOH, which occurred during 30 min of hyperoxia in the water group, was largely prevented in the groups that consumed red wine, glycerol+ethanol or fructose. In contrast to chronic hyperuricemia, generally considered as a risk factor for cardiovascular diseases and metabolic syndrome, acute increase of UA acts protectively against hyperoxia-induced oxidative stress and related increase of arterial stiffness in large peripheral arteries.
authors.affiliation: Department of Pharmacology, University of Split School of Medicine, Soltanska 2, 21000 Split, Croatia.
authors.forename: Jonatan
authors.initials: J
authors.lastname: Vukovic
authors.forename: Darko
authors.initials: D
authors.lastname: Modun
authors.forename: Danijela
authors.initials: D
authors.lastname: Budimir
authors.forename: Davorka
authors.initials: D
authors.lastname: Sutlovic
authors.forename: Ilza
authors.initials: I
authors.lastname: Salamunic
authors.forename: Ivan
authors.initials: I
authors.lastname: Zaja
authors.forename: Mladen
authors.initials: M
authors.lastname: Boban
mesh_terms: D000208:Acute Disease; D000328:Adult; D001628:Beverages; D015415:Biomarkers / Q000097:blood; D001916:Brachial Artery / Q000187:drug effects* / Q000503:physiopathology; D018592:Cross-Over Studies; D004548:Elasticity; D000431:Ethanol / Q000008:administration & dosage*; D005632:Fructose / Q000008:administration & dosage*; D005990:Glycerol / Q000008:administration & dosage*; D006801:Humans; D018496:Hyperoxia / Q000097:blood* / Q000503:physiopathology; D033461:Hyperuricemia / Q000097:blood* / Q000503:physiopathology; D008054:Lipid Peroxides / Q000097:blood; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects*; D017534:Radial Artery / Q000187:drug effects* / Q000503:physiopathology; D013858:Thiobarbiturates / Q000097:blood; D013997:Time Factors; D015854:Up-Regulation; D014527:Uric Acid / Q000097:blood*; D014920:Wine; D055815:Young Adult
chemical_list: D015415:Biomarkers; D008054:Lipid Peroxides; D013858:Thiobarbiturates; D014527:Uric Acid; D005632:Fructose; D000431:Ethanol; D005990:Glycerol