title: Selective separation of human serum albumin with copper(II) chelated poly(hydroxyethyl methacrylate) based nanoparticles.
abstract: Poly(hydroxyethyl methacrylate) (PHEMA) nanoparticles with an average size of 300 nm in diameter and with a polydispersity index of 1.156 were produced by surfactant free emulsion polymerization. Specific surface area of the PHEMA nanoparticles was found to be 996 m(2)/g. Metal-chelating ligand 3-(2-imidazoline-1-yl)propyl(triethoxysilane) (IMEO) was covalently attached to the PHEMA nanoparticles. IMEO content was 0.97 mmol IEMO/g. The morphology and properties of these nanoparticles were characterized with scanning electron microscopy, Fourier transform infrared spectroscopy and atomic force microscopy. The Cu2+-chelated PHEMA-IMEO nanoparticles were used in the adsorption-elution studies of human serum albumin (HSA) in a batch system. Maximum HSA adsorption amount of the Cu2+ chelated nanoparticles was 680 mg HSA/g. The PHEMA-IMEO-Cu2+ nanoparticles exhibited a quite high adsorption capacity and fast adsorption rate due to their high specific surface area and the absence of internal diffusion resistance.
authors.affiliation: Department of Chemistry, Hacettepe University, Ankara, Turkey.
authors.forename: Veyis
authors.initials: V
authors.lastname: Karakoc
authors.forename: Erkut
authors.initials: E
authors.lastname: Yilmaz
authors.forename: Deniz
authors.initials: D
authors.lastname: Türkmen
authors.forename: Nevra
authors.initials: N
authors.lastname: Oztürk
authors.forename: Sinan
authors.initials: S
authors.lastname: Akgöl
authors.forename: Adil
authors.initials: A
authors.lastname: Denizli
mesh_terms: D000327:Adsorption; D002614:Chelating Agents / Q000138:chemical synthesis / Q000737:chemistry*; D003300:Copper / Q000737:chemistry*; D006801:Humans; D053758:Nanoparticles / Q000737:chemistry*; D011102:Polyhydroxyethyl Methacrylate / Q000138:chemical synthesis / Q000737:chemistry*; D012709:Serum Albumin / Q000737:chemistry* / Q000302:isolation & purification*; D012821:Silanes / Q000737:chemistry; D017550:Spectroscopy, Fourier Transform Infrared; D013997:Time Factors
chemical_list: D002614:Chelating Agents; D012709:Serum Albumin; D012821:Silanes; D011102:Polyhydroxyethyl Methacrylate; D003300:Copper; C522569:triethoxysilane