Murine cytomegalovirus capsid assembly is dependent on US22 family gene M140 in infected macrophages.
Macrophages are an important target cell for infection with cytomegalovirus (CMV). A number of viral genes that either are expressed specifically in this cell type or function to optimize CMV replication in this host cell have now been identified. Among these is the murine CMV (MCMV) US22 gene family member M140, a nonessential early gene whose deletion (RVDelta140) leads to significant impairment in virus replication in differentiated macrophages. We have now determined that the defect in replication is at the stage of viral DNA encapsidation. Although the rate of RVDelta140 genome replication and extent of DNA cleavage were comparable to those for revertant virus, deletion of M140 resulted in a significant reduction in the number of viral capsids in the nucleus, and the viral DNA remained sensitive to DNase treatment. These data are indicative of incomplete virion assembly. Steady-state levels of both the major capsid protein (M86) and tegument protein M25 were reduced in the absence of the M140 protein (pM140). This effect may be related to the localization of pM140 to an aggresome-like, microtubule organizing center-associated structure that is known to target misfolded and overexpressed proteins for degradation. It appears, therefore, that pM140 indirectly influences MCMV capsid formation in differentiated macrophages by regulating the stability of viral structural proteins.
Hanson
Laura K
LK
Department of Microbiology and Molecular Cell Biology, Eastern Virginia Medical School, Norfolk, VA 23507, USA.
Slater
Jacquelyn S
JS
Cavanaugh
Victoria J
VJ
Newcomb
William W
WW
Bolin
Lisa L
LL
Nelson
Christine N
CN
Fetters
Lisa D
LD
Tang
Qiyi
Q
Brown
Jay C
JC
Maul
Gerd G
GG
Campbell
Ann E
AE
D000818:Animals; D002213:Capsid / Q000378:metabolism*; D002460:Cell Line; D015967:Gene Expression Regulation, Viral; D008264:Macrophages / Q000821:virology*; D051379:Mice; D005810:Multigene Family; D018146:Muromegalovirus / Q000235:genetics / Q000502:physiology*; D041681:NIH 3T3 Cells; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism*; D019065:Virus Assembly; D014779:Virus Replication
D014764:Viral Proteins