Inhibitory effects of flavonoids on TNF-alpha-induced IL-8 gene expression in HEK 293 cells.
Due to their multiple biological activities, flavonoids have gained attention as potentially useful therapeutics for a variety of diseases including cancer, cardiovascular diseases, and autoimmune diseases. In this study, we demonstrated that several flavonoids, including kaempferol, quercetin, fisetin, and chrysin block TNF-alpha induced IL-8 promoter activation and gene expression in HEK 293 cells. In addition, phosphorylation and degradation of IkappaBalpha and translocation of NF-kappaB p65 were inhibited by these flavonoids in TNF-alpha-stimulated HEK 293 cells. Furthermore, generation of reactive oxygen species (ROS) in response to TNF-alpha was reduced by the flavonoids. Moreover, although pretreatment with fisetin, quercetin, or chrysin decreased cell viability, kaempferol did not. Taken together, these findings suggest that kaempferol would be useful for the treatment of TNF-alpha-induced inflammatory diseases.
Lee
Soohyoung
S
Department of Microbiology, College of Medicine, Hallym University, Chuncheon 200-702, Korea.
Kim
Young-Jin
YJ
Kwon
Sanghoon
S
Lee
Younghee
Y
Choi
Soo Young
SY
Park
Jinseu
J
Kwon
Hyung-Joo
HJ
D000818:Animals; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D005419:Flavonoids / Q000378:metabolism / Q000494:pharmacology*; D005786:Gene Expression Regulation / Q000187:drug effects*; D006801:Humans; D007249:Inflammation / Q000276:immunology; D016209:Interleukin-8 / Q000235:genetics / Q000378:metabolism*; D016328:NF-kappa B / Q000378:metabolism; D011401:Promoter Regions, Genetic; D017382:Reactive Oxygen Species / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000378:metabolism*
D005419:Flavonoids; D016209:Interleukin-8; D016328:NF-kappa B; D017382:Reactive Oxygen Species; D014409:Tumor Necrosis Factor-alpha