ATG5 expression induced by MDMA (ecstasy), interferes with neuronal differentiation of neuroblastoma cells.
The amphetamine derivative 3, 4-methylenedioxymethamphetamine (MDMA) has become a popular recreational drug, and has also been shown to cause serotonergic neurotoxicity. This report shows that MDMA impairs brain development in a whole mouse embryo culture. The results of quantitative real-time PCR analysis showed that autophagy-related protein 5 (Atg5) expression is elevated in mouse embryo and neuroblastoma cells after MDMA treatment. This elevated Atg5 expression interferes with the neuronal differentiation of neuroblastoma cells such as SH-SY5Y and PC12 cells. Thus, our results suggest that the use of MDMA during pregnancy may impair neuronal development via an induction of Atg5 expression.
Chae
Myounghee
M
Korea Basic Science Institute, Gwangju Center, Gwangju 500-757, Korea.
Rhee
Gyu-Seek
GS
Jang
Ik-Soon
IS
Kim
Kwangsoo
K
Lee
Ji-Hae
JH
Lee
Seung-Yeul
SY
Kim
Minjung
M
Yang
Junyoung
J
Park
Junsoo
J
Lee
Seung-Hoon
SH
D000818:Animals; D001343:Autophagy / Q000187:drug effects; D000071187:Autophagy-Related Protein 5; D002454:Cell Differentiation / Q000187:drug effects*; D046149:Embryo Culture Techniques; D005260:Female; D047109:Fetal Development / Q000187:drug effects; D006801:Humans; D051379:Mice; D008869:Microtubule-Associated Proteins / Q000235:genetics* / Q000378:metabolism*; D018817:N-Methyl-3,4-methylenedioxyamphetamine / Q000494:pharmacology*; D009447:Neuroblastoma / Q000378:metabolism / Q000473:pathology; D009474:Neurons / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D016716:PC12 Cells; D011247:Pregnancy; D011506:Proteins / Q000235:genetics / Q000378:metabolism; D051381:Rats; D014212:Tretinoin / Q000378:metabolism
C502778:ATG5 protein, human; C495773:Atg5 protein, mouse; C545865:Atg5 protein, rat; D000071187:Autophagy-Related Protein 5; D008869:Microtubule-Associated Proteins; D011506:Proteins; D014212:Tretinoin; D018817:N-Methyl-3,4-methylenedioxyamphetamine