title: Correlations of HHV-6 viral load and plasma IL-6 concentration with HHV-6 encephalitis in allogeneic stem cell transplant recipients.
abstract: This study investigated factors associated with the development of human herpesvirus (HHV)-6 encephalitis. Among 111 enrolled subjects, 12 patients developed central nervous system (CNS) dysfunction. CNS dysfunction in four patients was found to have no association with HHV-6. The remaining eight patients displayed HHV-6 encephalitis (n=3), limbic encephalitis (HHV-6 DNA in cerebrospinal fluid was not examined; n=3) or CNS dysfunction because of an unidentified cause (n=2). Real-time PCR showed CNS dysfunction in the latter eight patients, which developed concomitant with the appearance of high plasma levels of HHV-6 DNA (> or =10(4) copies/ml). Overall, eight of the 24 patients with high-level HHV-6 DNA developed CNS dysfunction, whereas no patients developed CNS dysfunction potentially associated with HHV-6 infection if peak HHV-6 DNA was <10(4) copies/ml. We next analyzed plasma concentrations of IL-6, IL-10 and tumor necrosis factor-alpha among patients who displayed high-level plasma HHV-6 DNA and found elevated IL-6 concentrations preceding HHV-6 infection in patients who developed CNS dysfunction. (Mean+/-s.d.: 865.7+/-1036.3 pg/ml in patients with CNS dysfunction; 56.5+/-192.9 pg/ml in others; P=0.01). These results suggest that high-level HHV-6 load is necessary for the development of HHV-6 encephalitis, and systemic inflammatory conditions before HHV-6 infection form the preparatory conditions for progression to encephalopathy.
authors.affiliation: Blood Transfusion Center, Oita University Hospital, Oita, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Ogata
authors.forename: T
authors.initials: T
authors.lastname: Satou
authors.forename: R
authors.initials: R
authors.lastname: Kawano
authors.forename: S
authors.initials: S
authors.lastname: Takakura
authors.forename: K
authors.initials: K
authors.lastname: Goto
authors.forename: J
authors.initials: J
authors.lastname: Ikewaki
authors.forename: K
authors.initials: K
authors.lastname: Kohno
authors.forename: T
authors.initials: T
authors.lastname: Ikebe
authors.forename: T
authors.initials: T
authors.lastname: Ando
authors.forename: Y
authors.initials: Y
authors.lastname: Miyazaki
authors.forename: E
authors.initials: E
authors.lastname: Ohtsuka
authors.forename: Y
authors.initials: Y
authors.lastname: Saburi
authors.forename: T
authors.initials: T
authors.lastname: Saikawa
authors.forename: J
authors.initials: J
authors.lastname: Kadota
mesh_terms: D000293:Adolescent; D000328:Adult; D002648:Child; D004279:DNA, Viral / Q000097:blood; D018792:Encephalitis, Viral / Q000821:virology*; D005260:Female; D015654:Herpesvirus 6, Human / Q000235:genetics; D006801:Humans; D016753:Interleukin-10 / Q000097:blood; D015850:Interleukin-6 / Q000097:blood*; D008297:Male; D008875:Middle Aged; D019349:Roseolovirus Infections / Q000821:virology*; D033581:Stem Cell Transplantation / Q000009:adverse effects*; D014409:Tumor Necrosis Factor-alpha / Q000097:blood; D019562:Viral Load
chemical_list: D004279:DNA, Viral; D015850:Interleukin-6; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10