[Adenovirus mediated IL-24 gene expression inhibits growth of human glioma cell in vitro].
To investigate the inhibitory effect and anti-cancer mechanism of adenovirus mediated IL-24 gene expression on the human U251 glioma cell. U251 glioma cells were infected with Ad-IL-24 at various multiplicity of infection (MOIs). Cell proliferation was determined by MTT assay. Cell apoptosis was detected by flow cytometry and Hochest staining. The transcription of apoptosis-related genes was analyzed by reverse transcription-PCR (RT-PCR), and the expression of Cleaved Caspase-3 was analyzed by Western blotting. The result showed that the growth of U251 glioma cells was significantly inhibited by Ad-IL-24 at the MOI of 100. The apoptotic rate of U251 glioma cells was 42% 72 h after infection with Ad-IL-24. Four days after infection, the growth of the U251 glioma cells was inhibited to 50%. RT-PCR showed that Ad-IL-24 not only up-regulated expression of bax/bcl-2, ICE, C-myc, p53 and down-regulated the expression of HIF-1alpha, but also enhanced Caspase-3 activation, eventually resulting apoptosis. Taken together, these results suggest that infection of U251 glioma cells with Ad-IL-24 can inhibit growth and induce apoptosis significantly by the regulation of apoptosis-related genes.
Shan
Yunbo
Y
Department of Celluar and Molecular Biology, Medical School, Suzhou University, Suzhou 215123, China.
Sheng
Weihua
W
Xie
Yufeng
Y
Liu
Tielian
T
Jing
Yingying
Y
Hu
Zhiqing
Z
Yang
Jicheng
J
D000256:Adenoviridae / Q000235:genetics* / Q000378:metabolism; D017209:Apoptosis; D001932:Brain Neoplasms / Q000235:genetics / Q000473:pathology*; D049109:Cell Proliferation / Q000187:drug effects; D015316:Genetic Therapy; D005910:Glioma / Q000235:genetics / Q000473:pathology*; D006801:Humans; D007378:Interleukins / Q000235:genetics / Q000378:metabolism*; D011995:Recombination, Genetic; D014407:Tumor Cells, Cultured; D000099180:Interleukin-24
D007378:Interleukins; D000099180:Interleukin-24