LPA66 is required for editing psbF chloroplast transcripts in Arabidopsis.
To gain insight into the molecular mechanism of RNA editing, we have characterized the low psii accumulation66 (lpa66) Arabidopsis (Arabidopsis thaliana) mutant, which displays a high chlorophyll fluorescence phenotype. Its perturbed chlorophyll fluorescence is reflected in reduced levels of photosystem II (PSII) proteins. In vivo protein labeling showed that synthesis rates of the PSII reaction center protein D1/D2 were lower, and turnover rates of PSII core proteins higher, than in wild-type counterparts. The assembly of newly synthesized proteins into PSII occurs in the lpa66 mutant but with reduced efficiency compared with the wild type. LPA66 encodes a chloroplast protein of the pentatricopeptide repeat family. In lpa66 mutants, editing of psbF that converts serine to phenylalanine is specifically impaired. Thus, LPA66 is specifically required for editing the psbF transcripts in Arabidopsis, and the amino acid alternation due to lack of editing strongly affects the efficiency of the assembly of PSII complexes.
Cai
Wenhe
W
Photosynthesis Research Center, Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China.
Ji
Daili
D
Peng
Lianwei
L
Guo
Jinkui
J
Ma
Jinfang
J
Zou
Meijuan
M
Lu
Congming
C
Zhang
Lixin
L
D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000235:genetics* / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D002736:Chloroplasts / Q000378:metabolism; D003001:Cloning, Molecular; D008969:Molecular Sequence Data; D009154:Mutation; D045332:Photosystem II Protein Complex / Q000378:metabolism; D014176:Protein Biosynthesis / Q000235:genetics; D017434:Protein Structure, Tertiary; D017393:RNA Editing / Q000235:genetics / Q000502:physiology*; D012333:RNA, Messenger / Q000378:metabolism; D016415:Sequence Alignment
D029681:Arabidopsis Proteins; C543426:Lpa66 protein, Arabidopsis; D045332:Photosystem II Protein Complex; D012333:RNA, Messenger