Second harmonic microscopy of axonemes.
We performed Second Harmonic Microscopy of axonemes obtained from sea urchin sperm. Using polarization analysis and a trade-off between signal and photodamage, we were able to determine, for the first time to our knowledge, the nonlinear susceptibility chizxx/chixzx = 1.1+/-0.2 and chizzz/chixzx = 4+/-0.5 of axonemes.
Odin
Christophe
C
Institut of Physics of Rennes IPR/UMR CNRS 6251, University of Rennes I, Campus de Beaulieu, Bat 11A, 35042 Rennes Cedex, France. christophe.odin@univ-rennes1.fr
Heichette
Claire
C
Chretien
Denis
D
Le Grand
Yann
Y
D000818:Animals; D054468:Axoneme / Q000648:ultrastructure*; D002478:Cells, Cultured; D008856:Microscopy, Fluorescence / Q000379:methods*; D008858:Microscopy, Phase-Contrast / Q000379:methods*; D012617:Sea Urchins / Q000648:ultrastructure*