title: Expression of serotonin and its 5-HT1A receptor in canine cutaneous mast cell tumours.
abstract: Mast cells of a number of different animal species have been reported to contain serotonin (5-hydroxytryptamine; 5-HT), a monoamine capable of numerous and complex actions, which may include an impact on tumour growth. Limited previous studies have suggested that normal or neoplastic canine mast cells do not express 5-HT. In the present study, canine cutaneous mast cell tumours (MCTs) of Patnaik histological grades I-III were investigated immunohistochemically for expression of 5-HT and its receptor (R) 5-HT1A. The proportion of positively labelled cells and the intensity of labelling of individual cells were determined. Both 5-HT and the 5-HT1A receptor were expressed by non-neoplastic dermal mast cells and neoplastic mast cells. More neoplastic mast cells expressed 5-HT than the 5-HT1AR. Poorly differentiated tumours expressed fewer of both molecules, but the better differentiated mast cells at the periphery of such lesions had more consistent 5-HT expression. 5-HT and the 5-HT1A receptor may be involved in the differentiation of canine MCTs and in the microvascular complications associated with these neoplasms.
authors.affiliation: Albano Animal Hospital, Danderyd, Sweden. guka@djursjukhussthlm.se
authors.forename: G Kastengren
authors.initials: GK
authors.lastname: Fröberg
authors.forename: R
authors.initials: R
authors.lastname: Lindberg
authors.forename: M
authors.initials: M
authors.lastname: Ritter
authors.forename: K
authors.initials: K
authors.lastname: Nordlind
mesh_terms: D000818:Animals; D014408:Biomarkers, Tumor / Q000378:metabolism; D002471:Cell Transformation, Neoplastic / Q000378:metabolism / Q000473:pathology; D004283:Dog Diseases / Q000378:metabolism* / Q000473:pathology; D004285:Dogs; D019085:Fluorescent Antibody Technique, Direct / Q000379:methods / Q000662:veterinary; D007124:Immunoenzyme Techniques / Q000379:methods / Q000662:veterinary; D008407:Mast Cells / Q000378:metabolism; D012515:Mast-Cell Sarcoma / Q000378:metabolism / Q000473:pathology / Q000662:veterinary*; D009367:Neoplasm Staging / Q000662:veterinary; D044282:Receptor, Serotonin, 5-HT1A / Q000378:metabolism*; D012701:Serotonin / Q000378:metabolism*; D012867:Skin / Q000378:metabolism; D012878:Skin Neoplasms / Q000378:metabolism / Q000473:pathology / Q000662:veterinary*
chemical_list: D014408:Biomarkers, Tumor; D044282:Receptor, Serotonin, 5-HT1A; D012701:Serotonin