title: Practical implementation of adaptive optics in multiphoton microscopy.
abstract: A dedicated two-photon microscope incorporating adaptive-optic correction of specimen-induced aberrations is presented. Wavefront alteration of the scanning laser beam was achieved by use of a micromachined deformable mirror. Post scan head implementation produces a compact module compatible with the Bio-Rad MRC-600 scan head. Automatic aberration correction using feedback from the multiphoton fluorescence intensity allowed the adaptive optic to extend the imaging depth attainable in both artificial and biological refractive-index mismatched samples. With a 1.3-NA, x40, Nikon oil immersion objective, the imaging depth in water was extended from approximately 3.4 to 46.2 microm with a resolution defined by a FWHM axial point-spread function of 1.25 microm.
authors.forename: P
authors.initials: P
authors.lastname: Marsh
authors.forename: D
authors.initials: D
authors.lastname: Burns
authors.forename: J
authors.initials: J
authors.lastname: Girkin