Study of aerobic granulocyte functional activity in the presence of a radiosensitizer (metronidazole).
The in vitro effects of metronidazole on the production of reactive oxygen species by polymorphonuclear (PMN) cells were studied by means of nitroblue tetrazolium and luminol-dependent chemiluminescence. At therapeutic doses of metronidazole (4.98-24.86 microg/mL) significant inhibition of the production of reactive oxygen species was noted in both methods. The inhibitory effect was in a dose-dependent pattern. The data suggest a scavenging mechanism of metronidazole on reactive oxygen species generated by PMN.
Al-Krawi
E A A
EA
Department of Pharmacology, Basra College of Medicine, University of Basra, Basra, Iraq.
Al-Hashimi
A H M
AH
D000328:Adult; D000332:Aerobiosis / Q000187:drug effects / Q000502:physiology; D000890:Anti-Infective Agents / Q000737:chemistry / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004353:Drug Evaluation, Preclinical; D016166:Free Radical Scavengers / Q000494:pharmacology*; D006801:Humans; D007202:Indicators and Reagents; D008163:Luminescent Measurements; D008165:Luminol; D008795:Metronidazole / Q000737:chemistry / Q000494:pharmacology*; D008875:Middle Aged; D009249:NADP / Q000187:drug effects / Q000502:physiology; D009504:Neutrophils / Q000187:drug effects / Q000502:physiology; D009580:Nitroblue Tetrazolium; D018384:Oxidative Stress / Q000187:drug effects / Q000502:physiology; D010587:Phagocytosis / Q000187:drug effects / Q000502:physiology; D011838:Radiation-Sensitizing Agents / Q000737:chemistry / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000032:analysis / Q000037:antagonists & inhibitors*
D000890:Anti-Infective Agents; D016166:Free Radical Scavengers; D007202:Indicators and Reagents; D011838:Radiation-Sensitizing Agents; D017382:Reactive Oxygen Species; D008795:Metronidazole; D009580:Nitroblue Tetrazolium; D009249:NADP; D008165:Luminol