Human C21orf63 is a heparin-binding protein.
Human C21orf63 is a type-1 transmembrane protein of hitherto unknown function, with two repeats of putative 'galactose-binding lectin domains'. By using glycan microarray analysis and other assays, we found that human C21orf63 interacts with heparin and to a lesser extent with heparan sulphate. The C-terminal galactose-binding lectin domain of C21orf63 is necessary for heparin binding. The inability of other human proteins with galactose-binding lectin domains to interact with heparin suggests that heparin binding is a unique property of C21orf63. Results of real-time polymerase chain reaction and tissue immunostaining imply that C21orf63 is expressed on epithelia of various human tissues.
Mitsunaga
Kanae
K
Glycogene Function Team, Research Center for Medical Glycoscience, National Institute of Advanced Industrial Science and Technology (AIST), 1-1-1 Umezono, Tsukuba, Ibaraki 305-8568, Japan.
Harada-Itadani
Jun
J
Shikanai
Toshihide
T
Tateno
Hiroaki
H
Ikehara
Yuzuru
Y
Hirabayashi
Jun
J
Narimatsu
Hisashi
H
Angata
Takashi
T
D003001:Cloning, Molecular; D030562:Databases, Protein; D004847:Epithelial Cells / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D037161:Galectins; D005753:Gastric Mucosa / Q000378:metabolism; D015870:Gene Expression; D006025:Glycosaminoglycans / Q000378:metabolism; D006493:Heparin / Q000031:analogs & derivatives / Q000378:metabolism*; D006497:Heparan Sulfate / Q000378:metabolism; D006801:Humans; D055767:Immobilized Proteins / Q000378:metabolism; D037102:Lectins / Q000737:chemistry / Q000276:immunology / Q000378:metabolism*; D008565:Membrane Proteins / Q000737:chemistry / Q000276:immunology / Q000378:metabolism*; D046228:Microarray Analysis; D008856:Microscopy, Fluorescence; D050505:Mutant Proteins / Q000737:chemistry / Q000276:immunology / Q000378:metabolism; D009928:Organ Specificity; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000276:immunology / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013274:Stomach Neoplasms / Q000378:metabolism
D037161:Galectins; D006025:Glycosaminoglycans; D006493:Heparin; D006497:Heparan Sulfate; D055767:Immobilized Proteins; D037102:Lectins; D008565:Membrane Proteins; D050505:Mutant Proteins; D011993:Recombinant Fusion Proteins; C544758:EVA1C protein, human