title: Polygalasaponin G promotes neurite outgrowth of cultured neuron on myelin.
abstract: Myelin contains many axonal outgrowth inhibitory components which contribute to regeneration failure after neuronal injury in the mammalian central nervous system (CNS). In an attempt to develop small molecular agents to promote axonal outgrowth, we screened a compound library purified from traditional Chinese herbs, and found a small molecular compound polygalasaponin G (PS-G), extracted from Polygala japonica, which has a potent neurotrophic activity on PC12 cells and cultured cortical neurons. We reported, to our knowledge for the first time, that PS-G could promote neurite outgrowth of neurons cultured on the myelin substrates and inhibit the activation of RhoA. Thus, our results could represent a therapeutic approach to improve axon regeneration after CNS injuries.
authors.affiliation: Institute of Neuroscience and Key Laboratory of Molecular Neurobiology, Ministry of Education, Second Military Medical University, Shanghai 200433, China.
authors.forename: Xiao-Hui
authors.initials: XH
authors.lastname: Xu
authors.forename: Jian-Feng
authors.initials: JF
authors.lastname: Zhou
authors.forename: Ting-Zhao
authors.initials: TZ
authors.lastname: Li
authors.forename: Zhao-Huan
authors.initials: ZH
authors.lastname: Zhang
authors.forename: Lei
authors.initials: L
authors.lastname: Shan
authors.forename: Zheng-Hua
authors.initials: ZH
authors.lastname: Xiang
authors.forename: Zhong-Wang
authors.initials: ZW
authors.lastname: Yu
authors.forename: Wei-Dong
authors.initials: WD
authors.lastname: Zhang
authors.forename: Cheng
authors.initials: C
authors.lastname: He
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D002478:Cells, Cultured; D002531:Cerebellum / Q000166:cytology / Q000502:physiology; D004305:Dose-Response Relationship, Drug; D020287:In Situ Nick-End Labeling / Q000379:methods; D009185:Myelin Proteins / Q000494:pharmacology; D009186:Myelin Sheath / Q000502:physiology*; D020932:Nerve Growth Factor / Q000494:pharmacology; D016501:Neurites / Q000187:drug effects*; D055495:Neurogenesis / Q000187:drug effects / Q000502:physiology; D009474:Neurons / Q000166:cytology*; D000070798:Nogo Proteins; D016716:PC12 Cells; D031801:Polygala / Q000737:chemistry*; D051381:Rats; D012503:Saponins / Q000737:chemistry / Q000494:pharmacology*; D020742:rhoA GTP-Binding Protein / Q000378:metabolism
chemical_list: D009185:Myelin Proteins; D000070798:Nogo Proteins; C000603197:Rtn4 protein, rat; D012503:Saponins; C543171:polygalasaponin G; D020932:Nerve Growth Factor; D020742:rhoA GTP-Binding Protein