title: A nucleolar protein RRS1 contributes to chromosome congression.
abstract: We report here the functional analysis of human Regulator of Ribosome Synthesis 1 (RRS1) protein during mitosis. We demonstrate that RRS1 localizes in the nucleolus during interphase and is distributed at the chromosome periphery during mitosis. RNA interference experiments revealed that RRS1-depleted cells show abnormalities in chromosome alignment and spindle organization, which result in mitotic delay. RRS1 knockdown also perturbs the centromeric localization of Shugoshin 1 and results in premature separation of sister chromatids. Our results suggest that a nucleolar protein RRS1 contributes to chromosome congression.
authors.affiliation: Department of Biotechnology, Graduate School of Engineering, Osaka University, Suita, Osaka, Japan.
authors.forename: Arni E
authors.initials: AE
authors.lastname: Gambe
authors.forename: Sachihiro
authors.initials: S
authors.lastname: Matsunaga
authors.forename: Hideaki
authors.initials: H
authors.lastname: Takata
authors.forename: Rika
authors.initials: R
authors.lastname: Ono-Maniwa
authors.forename: Akiko
authors.initials: A
authors.lastname: Baba
authors.forename: Susumu
authors.initials: S
authors.lastname: Uchiyama
authors.forename: Kiichi
authors.initials: K
authors.lastname: Fukui
mesh_terms: D001483:Base Sequence; D018797:Cell Cycle Proteins / Q000378:metabolism; D002466:Cell Nucleolus / Q000378:metabolism / Q000648:ultrastructure; D023902:Chromosome Pairing; D020090:Chromosome Segregation; D002877:Chromosomes, Human / Q000378:metabolism* / Q000648:ultrastructure; D006367:HeLa Cells; D006801:Humans; D008856:Microscopy, Fluorescence; D008938:Mitosis; D009687:Nuclear Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics; D016601:RNA-Binding Proteins; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D008941:Spindle Apparatus / Q000378:metabolism / Q000648:ultrastructure
chemical_list: D018797:Cell Cycle Proteins; D009687:Nuclear Proteins; D034741:RNA, Small Interfering; D016601:RNA-Binding Proteins; C405526:RRS1 protein, human; D011994:Recombinant Proteins; C496644:SGO1 protein, human