[Determination of 5-hydroxymethyl-furfural in root of Achyranthes bidentata expressing different degree of floating sugar].
OBJECTIVE
To study the relation between the content of 5-hydroxymethyl-furfural (5-HMF) and the degree of floating sugar in root of Achyranthes bidentata.


METHODS
An HPLC method was applied with a Waters Symmetry C18 3.9 mm x 150 mm (5 microm) column by using methanol-water (12:88) as the mobile phase at a flow rate of 1.0 mL x min(-1) and a UV detection of 280 nm.


RESULTS
Along with the degree's deepening of floating sugar, the content of 5-HMF varied with the different shades of the sample. The content was 10 times higher in the black sample (highest degree of flowing suger) than that in the yellowish sample (normal). The concentrations of 5-HMT in five yellowish samples of roots of A. bidentata were 0.162 mg x g(-1) to 0.332 mg x g(-1).


CONCLUSIONS
The content increasing of 5-HMF in the root of A. bidentata was related to the degree of flowing sugar.
Liu
Zhenli
Z
Institute of Chinese Medicine Basic Theory, China Academy of Chinese Medical Sciences, Beijing 100700, China.
Song
Zhiqian
Z
Wang
Chun
C
Lv
Shuyi
S
Chao
Zhimao
Z
D029044:Achyranthes / Q000737:chemistry*; D002241:Carbohydrates / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal / Q000032:analysis; D005662:Furaldehyde / Q000032:analysis*; D018517:Plant Roots / Q000737:chemistry*
D002241:Carbohydrates; D004365:Drugs, Chinese Herbal; D005662:Furaldehyde