Effect of vitamin E on ectonucleotidase activities in synaptosomes and platelets and parameters of oxidative stress in rats experimentally demyelinated.
NTPDase and 5'-nucleotidase activities in synaptosomes and platelets and oxidative stress parameters, such as TBARS levels, non-protein thiols and catalase activity were analyzed in rats submitted to demyelination by ethidium bromide (EB) and treated with vitamin E. The following groups were studied: I control (saline); II (canola oil); III (vitamin E); IV (EB) and V (EB and vitamin E). 2mg/kg of vitamin E were injected intraperitoneally in animals from groups III and V for seven days. After this time, the animals were submitted to euthanasia and samples were collected for biochemical assays. The results showed that NTPDase and 5'-nucleotidase activities were significantly increased in synaptosomes and platelets of rats from group IV when compared with the groups I, II, III and V (p<0.05). When demyelinated rats were treated with vitamin E (group V), NTPDase activity in synaptosomes and platelets was reduced to control level, while 5'-nucleotidase activity was significantly increased in relation to the control group (p<0.05). TBARS levels and non-protein thiols were significantly increased in group IV (p<0.05), while catalase activity was significantly decreased in this group when compared with the control group (p<0.05). No differences in TBARS levels, non-protein thiols and catalase activity were observed in groups I, II, III and V. These findings demonstrate that ectonucleotidase activities in synaptosomes and platelets and some parameters of oxidative stress were altered after a demyelinating event on the nervous system and that treatment with vitamin E modulated adenine nucleotide hydrolysis and altered oxidative stress parameters in this experimental condition.
Spanevello
Roselia
R
Departamento de Bioquímica, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, Rua Ramiro Barcellos, 2600-Anexo, Porto Alegre, RS, Brazil.
Mazzanti
Cinthia M
CM
Schmatz
Roberta
R
Bagatini
Margarete
M
Stefanello
Naiara
N
Correa
Maisa
M
Kaizer
Rosilene
R
Maldonado
Paula
P
Mazzanti
Alexandre
A
Graça
Dominguita L
DL
Martins
Tessie B
TB
Danesi
Cristiane
C
Morsch
Vera M
VM
Schetinger
Maria Rosa C
MR
D015720:5'-Nucleotidase / Q000378:metabolism*; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D001792:Blood Platelets / Q000187:drug effects / Q000201:enzymology; D001921:Brain / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D004996:Ethidium / Q000494:pharmacology; D005260:Female; D006801:Humans; D009186:Myelin Sheath / Q000473:pathology*; D018384:Oxidative Stress; D011755:Pyrophosphatases / Q000378:metabolism*; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D013574:Synaptosomes / Q000187:drug effects / Q000201:enzymology; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism; D014810:Vitamin E / Q000494:pharmacology*
D000975:Antioxidants; D017392:Thiobarbituric Acid Reactive Substances; D014810:Vitamin E; D015720:5'-Nucleotidase; D011755:Pyrophosphatases; C092064:ectonucleotide pyrophosphohydrolase; D004996:Ethidium