title: Development of a high-sensitivity chromatographic separation system for pyridylaminated aldopentoses and aldohexoses.
abstract: A rare sugar is considered to be a monosaccharide rarely found in nature. To investigate their natural distribution and biological roles, a robust analytical system must be used to isolate, identify, and quantify them. Herein, we report the development of such a system that can specifically quantify and chromatographically separate four aldopentoses and eight aldohexoses tagged with 2-aminopyridine. Purified monosaccharides derivatized with a pyridylamino moiety (PA-monosaccharides) are first chromatographed over a high-performance anion-exchange resin. But, because two of the PA-aldohexoses used in this study, PA-talose and PA-idose, co-elute with the common saccharides, PA-glucose and PA-mannose, respectively, a second chromatographic step, reversed-phase high-performance liquid chromatography, is used to completely separate them. Thus, as shown by the results of this study, chromatographic separation of PA-monosaccharides is achievable and provides a quantitative measurement of common and rare isomeric aldopentoses and aldohexoses.
authors.affiliation: Department of Functional Glycomics, Life Science Research Center, Kagawa University, 1750-1 Ikenobe, Miki-cho, Kita-gun, Kagawa 761-0793, Japan.
authors.forename: Shin-ichi
authors.initials: S
authors.lastname: Nakakita
authors.forename: Kayo
authors.initials: K
authors.lastname: Hasehira
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Hosokawa
authors.forename: Masaaki
authors.initials: M
authors.lastname: Tokuda
authors.forename: Ken
authors.initials: K
authors.lastname: Izumori
authors.forename: Kaoru
authors.initials: K
authors.lastname: Takegawa
authors.forename: Jun
authors.initials: J
authors.lastname: Hirabayashi
mesh_terms: D000631:Aminopyridines / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D009005:Monosaccharides / Q000737:chemistry / Q000302:isolation & purification*; D012680:Sensitivity and Specificity
chemical_list: D000631:Aminopyridines; D009005:Monosaccharides