Evaluation of the genetic variability of orchid fleck virus by single-strand conformational polymorphism analysis and nucleotide sequencing of a fragment from the nucleocapsid gene.
The variability of a fragment of the nucleocapsid gene of orchid fleck virus (OFV) was investigated by single-strand conformational polymorphism (SSCP) analysis and nucleotide sequencing. Forty-eight samples of 18 genera of orchids were collected from Brazil, Costa Rica and Australia. The SSCP analysis yielded six different band patterns, and phylogenetic analysis based on the nucleotide fragment sequence obtained in this work and six available in GenBank showed two different groups, one with isolates 023Germany and So-Japan, and other with the rest of the isolates. None of the analyses showed geographic correlation among the Brazilian strains. The data obtained in this study showed a low genetic variation in this region of the genome; the d(N)/d(S) ratio of 0.251-0.405 demonstrated a negative selective pressure that maintains the stability of the analyzed fragments.
Kubo
Karen Sumire
KS
Centro APTA Citros Sylvio Moreira/IAC, Rod. Anhanguera Km 158, CP 4, Cordeirópolis, SP, 13490-970, Brazil. karenkubo@yahoo.com.br
Stuart
R M
RM
Freitas-Astúa
J
J
Antonioli-Luizon
R
R
Locali-Fabris
E C
EC
Coletta-Filho
H D
HD
Machado
M A
MA
Kitajima
E W
EW
D001315:Australia; D001938:Brazil; D016000:Cluster Analysis; D003364:Costa Rica; D005838:Genotype; D019251:Nucleocapsid / Q000235:genetics*; D029595:Orchidaceae / Q000821:virology*; D010802:Phylogeny; D018807:Polymorphism, Single-Stranded Conformational; D012209:Rhabdoviridae / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D017422:Sequence Analysis, DNA