Electrochemistry of cytochromes p450: analysis of current-voltage characteristics of electrodes with immobilized cytochromes p450 for the screening of substrates and inhibitors.
In the current study, an approach to elucidating the substrate specificity of cytochromes P450 based on the analysis of current-voltage characteristics of voltammograms and amperograms is proposed. Data on the electrochemical behavior of bioelectrodes with immobilized cytochromes P450 2B4, 1A2, 3A4, 11A1 (P450scc), and 51b1 (Mycobacterium tuberculosis sterol 14alpha-demethylase or CYP51 MT) in the presence of typical substrates and inhibitors for these hemoprotein forms are reported. Immobilization of the enzymes was accomplished by using graphite screen-printed electrodes modified with gold nanoparticles and with the synthetic membrane-like compound didodecyldimethylammonium bromide. The method of electro-analysis can be applied to the search of potential substrates and inhibitors of cytochromes P450 and to creation of multichannel electrochemical plates (chips, panels) with immobilized cytochromes P450.
Shumyantseva
V V
VV
Institute of Biomedical Chemistry, Russian Academy of Medical Sciences, Moscow, 119121, Russia. viktoria.shumyantseva@ibmc.msk.ru
Bulko
T V
TV
Kuznetsova
G P
GP
Samenkova
N F
NF
Archakov
A I
AI
D000818:Animals; D065607:Cytochrome P-450 Enzyme Inhibitors; D003577:Cytochrome P-450 Enzyme System / Q000737:chemistry* / Q000378:metabolism; D004353:Drug Evaluation, Preclinical / Q000379:methods*; D004563:Electrochemistry / Q000379:methods*; D004566:Electrodes; D004791:Enzyme Inhibitors / Q000737:chemistry*; D004800:Enzymes, Immobilized / Q000037:antagonists & inhibitors* / Q000737:chemistry* / Q000378:metabolism; D009169:Mycobacterium tuberculosis / Q000737:chemistry / Q000201:enzymology; D011817:Rabbits; D013379:Substrate Specificity
D065607:Cytochrome P-450 Enzyme Inhibitors; D004791:Enzyme Inhibitors; D004800:Enzymes, Immobilized; D003577:Cytochrome P-450 Enzyme System