Flow on the right side of the gastrocoel roof plate is dispensable for symmetry breakage in the frog Xenopus laevis.
Leftward flow of extracellular fluid breaks the bilateral symmetry of most vertebrate embryos, manifested by the ensuing asymmetric induction of Nodal signaling in the left lateral plate mesoderm (LPM). Flow is generated by rotational beating of polarized monocilia at the posterior notochord (PNC; mammals), Kupffer's vesicle (KV; teleost fish) and the gastrocoel roof plate (GRP; amphibians). To manipulate flow in a defined way we cloned dynein heavy chain genes dnah5, 9 and 11 in Xenopus. dnah9 expression was closely related to motile cilia from neurulation onwards. Morphant tadpoles showed impaired epidermal ciliary beating. Leftward flow at the GRP was absent, resulting in embryos with loss of asymmetric marker gene expression. Remarkably, unilateral knockdown on the right side of the GRP did not affect laterality, while left-sided ablation of flow abolished marker gene expression. Thus, flow was required exclusively on the left side of the GRP to break symmetry in the frog. Our data suggest that the substrate of flow is generated within the GRP and not at its margin, disqualifying Nodal as a candidate morphogen.
Vick
Philipp
P
University of Hohenheim, Institute of Zoology, Stuttgart, Germany.
Schweickert
Axel
A
Weber
Thomas
T
Eberhardt
Melanie
M
Mencl
Stine
S
Shcherbakov
Denis
D
Beyer
Tina
T
Blum
Martin
M
D000818:Animals; D019521:Body Patterning / Q000502:physiology; D002923:Cilia / Q000502:physiology; D045604:Extracellular Fluid / Q000502:physiology*; D055785:Gene Knockdown Techniques; D007814:Larva; D055458:Left-Right Determination Factors / Q000502:physiology*; D008648:Mesoderm / Q000196:embryology / Q000254:growth & development / Q000502:physiology*; D009672:Notochord / Q000196:embryology / Q000254:growth & development / Q000502:physiology; D029867:Xenopus Proteins / Q000235:genetics / Q000502:physiology*; D014982:Xenopus laevis / Q000196:embryology* / Q000254:growth & development / Q000502:physiology
D055458:Left-Right Determination Factors; D029867:Xenopus Proteins