[Extraction process and content determination of total organic acids from Pothos chinensis].
OBJECTIVE
To study the optimum extracting process of total organic acids from Pothos chinensis and establish a determination method for total organic acids.


METHODS
By orthogonal design, we investigated the effects of 4 factors containing concentration of ethanol, ratio of solid to liquid, frequency of extraction and time of extraction. After the extraction, the determination was done with potential titration.


RESULTS
The best extraction conditions were using 75% (V/V) ethanol as extractor with the radio of 1:10, and extracting for 2.5 h and 3 times. The determination method was simple, reproducible and suitable.


CONCLUSIONS
These results provide certain scientific basis for further efficacy of research and development of Pothos chinensis.
Yin
Wen-qing
WQ
Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources, Ministry of Education of China, College of Chemistry and Chemical Engineering, Guangxi Normal University, Guilin 541004, China. yinwq0000@yahoo.com.cn
Zhang
Yan
Y
Song
Xin-ming
XM
Zeng
Li
L
D000704:Analysis of Variance; D031057:Arisaema / Q000737:chemistry*; D002264:Carboxylic Acids / Q000032:analysis / Q000302:isolation & purification*; D000431:Ethanol / Q000008:administration & dosage; D010946:Plants, Medicinal / Q000737:chemistry*; D011199:Potentiometry; D011786:Quality Control; D015203:Reproducibility of Results; D013678:Technology, Pharmaceutical / Q000379:methods*; D013696:Temperature; D013997:Time Factors
D002264:Carboxylic Acids; D000431:Ethanol