A comparative study of tumor-suppression effects of enterotoxin B and Chalone 19-peptide on experimental gastric carcinoma.
OBJECTIVE
In this paper, the in vivo tumor suppression effects of Staphyococal enterotoxin B (SEB) and Chalone 19-peptide, a form of Tumstain with modified coding sequence, on poorly differentiated human gastric carcinoma cells were compared.


METHODS
Animal model for studying human gastric carcinoma was established by injecting tumor cells (Human poorly differentiated gastric carcinoma cell line, SGC-7901) underneath the gastric serosa of BALB/c nude mice.


RESULTS
Results demonstrated that SEB induced tumor cell death on a large scale and destroyed surrounding normal tissue at the same time, leading to tumor cluster breaking down and seeding. SEB had no effect on lymphovascular metastasis. The administration of 19-peptide on gastric carcinoma resulted in sheets-like tumor central necrosis, decreased angiogenesis and a moderate tumor infiltration into surrounding tissue without distant metastasis. Therefore, both SEB and 19-peptide could suppress the local growth, distant metastasis and invasion of poorly differentiated gastric carcinoma cells into surrounding tissues.


CONCLUSIONS
Data suggested that this model could effectively simulate the microenvironment of human gastric carcinoma, hence providing a platform for study on this cancer.
Huan
Yuanxi
Y
Abdominal Surgery, The Tumor Hospital of Harbin Medical University, Harbin 150081, China. jinxm55@163.com
He
Yan
Y
Liu
Miao
M
Jin
Xiaoming
X
Li
Xingjie
X
Liu
Xinghan
X
Zhang
Qifan
Q
D000818:Animals; D045744:Cell Line, Tumor; D050150:Chalones / Q000494:pharmacology*; D004768:Enterotoxins / Q000494:pharmacology*; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009361:Neoplasm Invasiveness; D009368:Neoplasm Transplantation; D013274:Stomach Neoplasms / Q000188:drug therapy* / Q000473:pathology*
D050150:Chalones; D004768:Enterotoxins