House dust mite major allergen Der f 1 enhances proinflammatory cytokine and chemokine gene expression in a cell line of canine epidermal keratinocytes.
House dust mite (HDM) allergens are the most common allergens involved in the induction of IgE-mediated hypersensitivity. Recently, epicutaneous sensitization with HDM allergens has been emphasized in the development of atopic dermatitis (AD); however, direct stimulation of canine keratinocytes by mite allergens has not been well investigated. In the present study, we investigated the effects of Der f 1, a major allergen of Dermatophagoides farinae, on cytokine and chemokine gene expression in a canine keratinocyte cell line, CPEK. CPEK constitutively expressed mRNA for TNF-alpha, IL-12p35, IL-18, GM-CSF, TGF-beta, IL-8/CXCL8, TARC/CCL17, CTACK/CCL27 and MEC/CCL28. Of all the cytokines and chemokines investigated in CPEK, transcription levels of GM-CSF, IL-8/CXCL8 and TNF-alpha mRNA were significantly enhanced by stimulation with Der f 1. The present results suggest that Der f 1 can directly augment inflammatory cytokine and chemokine production from keratinocytes, and may initiate allergic inflammation independently of Type-I hypersensitivity.
Maeda
Shingo
S
Department of Veterinary Medicine, Faculty of Applied Biological Sciences, Gifu University, Gifu, Japan.
Maeda
Sadatoshi
S
Shibata
Sanae
S
Chimura
Naoki
N
Fukata
Tsuneo
T
D000485:Allergens / Q000008:administration & dosage*; D000818:Animals; D039741:Antigens, Dermatophagoides / Q000008:administration & dosage*; D060829:Arthropod Proteins; D001483:Base Sequence; D002460:Cell Line; D018925:Chemokines / Q000235:genetics*; D003546:Cysteine Endopeptidases; D016207:Cytokines / Q000235:genetics*; D017931:DNA Primers / Q000235:genetics; D004285:Dogs / Q000235:genetics* / Q000276:immunology*; D015870:Gene Expression; D018836:Inflammation Mediators / Q000378:metabolism; D015603:Keratinocytes / Q000276:immunology*; D039981:Pyroglyphidae / Q000276:immunology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism
D000485:Allergens; D039741:Antigens, Dermatophagoides; D060829:Arthropod Proteins; D018925:Chemokines; D016207:Cytokines; D017931:DNA Primers; D018836:Inflammation Mediators; D012333:RNA, Messenger; D003546:Cysteine Endopeptidases; C451159:Dermatophagoides farinae antigen f 1