[Determination of four acetophenones in Radix Cynanchi bungei by high performance liquid chromatography-photodiode array detection].
A high performance liquid chromatographic (HPLC) method was developed for the determination of four acetophenones, namely 4-hydroxyacetophenone (I), 2,5-dihydroxyacetophenone (II), baishouwubenzophenone (III) and 2,4-dihydroxyacetophenone (IV), in Radix Cynanchi bungei. The HPLC with photodiode array (PDA) detection was carried out on a Symmetry-C18 (4.6 mm x 250 mm, 5 microm) column with CH3OH-H2O (26 : 74, v/v) as the mobile phase at the flow rate of 1.0 mL/min and 30 degrees C. The compounds I , III, IV were monitored at 280 nm, and the compound II was at 224 nm. Four acetophenones (I - IV) had good linearities (r = 0.999 6 - 0.999 9) at the ranges of 0.080 - 0.560 microg, 0.080 -0.560 microg, 0.100 - 0.700 microg and 0.092 -0.644 microg, respectively. The average recoveries were from 98.0% to 104.0% with relative standard deviations (RSD) ranging from 0.8% to 2.6%. The method is of quick, simple and accurate. The method can be used for the quality control of this product.
Sun
Yinshi
Y
College of Agronomy, Shandong Agricultural University, Taian 271018, China.
Liu
Zhengbo
Z
Wang
Jianhua
J
Wang
Ying
Y
Zhou
Hongying
H
Zhang
Wenli
W
Wang
Jun
J
Qiao
Gui
G
D000098:Acetophenones / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D031105:Cynanchum / Q000737:chemistry*; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D018517:Plant Roots / Q000737:chemistry*; D011786:Quality Control
D000098:Acetophenones; D004365:Drugs, Chinese Herbal; C019339:2,6-dihydroxyacetophenone; C078634:2,4-dihydroxyacetophenone; C031335:4-hydroxyacetophenone