title: Pentosidine accumulates in the aging vitreous body: a gender effect.
abstract: The human vitreous body undergoes structural changes with aging. This can be followed by a posterior vitreous detachment, which can result in ocular pathology. As in many collagenous tissues, age-related changes in the vitreous could be caused by the formation of advanced glycation end products (AGEs). The goal of this study was to find out whether the AGE pentosidine accumulates in the human vitreous with aging. With this data we were able to estimate the half-life of vitreous collagen. Furthermore, we analyzed whether there was a gender difference in pentosidine accumulation, as this was seen in other tissues as well. Using high performance liquid chromatography, pentosidine contents were determined in whole vitreous bodies and in separate parts of vitreous bodies, which were all obtained from human donor eyes. Our results show that pentosidine accumulates in the human vitreous. From the rate of accumulation we could roughly estimate that vitreous collagen has as a similar or shorter half-life compared to skin collagen. This supports the concept of collagen turnover in the vitreous. In general, the female vitreous experiences a faster pentosidine accumulation than the male vitreous, and most of the pentosidine accumulation in the former occurs after 50 years of age.
authors.affiliation: University Medical Center Groningen, University of Groningen, Department of Ophthalmology, P.O. Box 30.001, 9700 RB Groningen, The Netherlands. m.van.deemter@ohk.umcg.nl
authors.forename: M
authors.initials: M
authors.lastname: van Deemter
authors.forename: T L
authors.initials: TL
authors.lastname: Ponsioen
authors.forename: R A
authors.initials: RA
authors.lastname: Bank
authors.forename: J M M
authors.initials: JM
authors.lastname: Snabel
authors.forename: R J
authors.initials: RJ
authors.lastname: van der Worp
authors.forename: J M M
authors.initials: JM
authors.lastname: Hooymans
authors.forename: L I
authors.initials: LI
authors.lastname: Los
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000375:Aging / Q000378:metabolism*; D001120:Arginine / Q000031:analogs & derivatives* / Q000378:metabolism; D002648:Child; D002675:Child, Preschool; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D002924:Ciliary Body / Q000378:metabolism; D005260:Female; D017127:Glycation End Products, Advanced / Q000378:metabolism; D006801:Humans; D008239:Lysine / Q000031:analogs & derivatives* / Q000378:metabolism; D008297:Male; D008593:Menopause / Q000378:metabolism; D008875:Middle Aged; D012727:Sex Characteristics; D014822:Vitreous Body / Q000378:metabolism*; D055815:Young Adult
chemical_list: D017127:Glycation End Products, Advanced; D001120:Arginine; C062187:pentosidine; D008239:Lysine