title: Identification and cell cycle-dependent localization of nine novel, genuine centrosomal components in Dictyostelium discoideum.
abstract: The centrosome is the main microtubule-organizing center and constitutes the largest protein complex in a eukaryotic cell. The Dictyostelium centrosome is an established model for acentriolar centrosomes and it consists of a layered core structure surrounded by a so-called corona, which harbors microtubule nucleation complexes. We have identified 34 new centrosomal candidate proteins through mass spectrometrical analysis of the proteome of isolated Dictyostelium centrosomes. Here we present a characterization of 12 centrosomal candidate proteins all featuring coiled coil regions and low expression levels, which are the most common attributes of centrosomal proteins. We used GFP fusion proteins to localize the candidate proteins in whole cells and on microtubule-free, isolated centrosomes. Thus we were able to identify nine new genuine centrosomal proteins including a putative orthologue of Cep192, an interaction partner of polo-like kinase 4 in human centriole biogenesis. In this respect, centrosomal localization of the only polo-like kinase in Dictyostelium, Plk, is also shown in this work. Using confocal deconvolution microscopy, four components, CP39, CP55, CP75, and CP91 could be clearly assigned to the so far almost uncharacterized centrosomal core structure, while CP148 and Cep192 localized to a zone between that of corona marker and core proteins. Finally, CP103 and CP248 were constituents of the corona. In contrast, NE81 was localized at the nuclear envelope and three others, an orthologue of the spindle checkpoint component Mad1, the novel Cenp68, and the centrosomal CP248 were observed at the centromeres, which are clustered and linked to the centrosome throughout the entire cell cycle.
authors.affiliation: Department of Cell Biology, University of Potsdam, Potsdam-Golm, Germany. Irene.schulz@uni-potsdam.de
authors.forename: Irene
authors.initials: I
authors.lastname: Schulz
authors.forename: Alexander
authors.initials: A
authors.lastname: Erle
authors.forename: Ralph
authors.initials: R
authors.lastname: Gräf
authors.forename: Anne
authors.initials: A
authors.lastname: Krüger
authors.forename: Heiner
authors.initials: H
authors.lastname: Lohmeier
authors.forename: Sascha
authors.initials: S
authors.lastname: Putzler
authors.forename: Matthias
authors.initials: M
authors.lastname: Samereier
authors.forename: Sebastian
authors.initials: S
authors.lastname: Weidenthaler
mesh_terms: D000818:Animals; D002453:Cell Cycle / Q000235:genetics*; D018385:Centrosome / Q000378:metabolism* / Q000648:ultrastructure; D002868:Chromosomal Proteins, Non-Histone / Q000235:genetics / Q000378:metabolism; D004023:Dictyostelium / Q000166:cytology / Q000235:genetics* / Q000648:ultrastructure; D006801:Humans; D018613:Microscopy, Confocal; D017346:Protein Serine-Threonine Kinases / Q000235:genetics / Q000378:metabolism; D015800:Protozoan Proteins / Q000235:genetics* / Q000378:metabolism; D020539:Sequence Analysis, Protein
chemical_list: C526596:Cep192 protein, human; D002868:Chromosomal Proteins, Non-Histone; D015800:Protozoan Proteins; C490219:PLK4 protein, human; D017346:Protein Serine-Threonine Kinases