[Adhesion and growth of human periodontal ligament cells on hyaluronic acid/collagen scaffold].
OBJECTIVE
The adhesion and growth of human periodontal ligament cells (PDLC) on collagen (Col), hyaluronic acid (HA) and HA/Col scaffolds were studied to evaluate the feasibility of HA/Col as a scaffold material in periodontal tissue engineering.


METHODS
Human PDLC cultured in vitro was collected and seeded on Col, HA, HA/Col scaffolds crosslinked by carbodiimide. The influences of scaffolds on cell adhesion and growth were observed by MTT assay. The growth of human PDLC on scaffolds was observed through inverted phase contrast microscope and scanning electron microscope (SEM).


RESULTS
The result of MTT assay demonstrated that except the seventh day there was obviously differentiation between each group Col, HA, HA/Col scaffolds at the first, second and fourth day, and HA/Col group was higher than control group all the time. Human PDLC successfully grew on scaffolds.


CONCLUSIONS
HA/Col scaffold was more suitable for human PDLC adhesion compared with Col scaffold and HA scaffold. This indicates that HA/Col scaffold might be a potential ideal candidate for periodontal tissue engineering.
Wang
Li-Xia
LX
State Key Laboratory of Oral Diseases, Sichuan University, Chengdu 610041, China.
Zhao
Huan
H
Jiang
Bo
B
Ding
Yi
Y
D002448:Cell Adhesion; D002454:Cell Differentiation; D002478:Cells, Cultured; D003094:Collagen; D006801:Humans; D006820:Hyaluronic Acid; D010513:Periodontal Ligament; D023822:Tissue Engineering; D054457:Tissue Scaffolds
D006820:Hyaluronic Acid; D003094:Collagen