title: Detection of human papilloma virus DNA in seven cases of focal epithelial hyperplasia in Iran.
abstract: BACKGROUND
  Focal epithelial hyperplasia (FEH), also known as Heck's disease, is a very rare disease of the oral cavity especially in Asia. It is a disease of children and young adults. Various causes have been implicated but in majority of cases FEH is caused by some subtypes of human papilloma virus (HPV) especially 13 and 32. Polymerase chain reaction (PCR) is a useful tool to identify HPV in FEH as it is a rapid and sensitive method.
  
  
  OBJECTIVE
  This study was designed to determine special HPV subtypes in seven cases of Heck's disease referring to our department by using PCR analysis.
  
  
  METHODS
  Paraffin sections of seven patients clinically diagnosed as FEH with compatible histhopathological features underwent DNA extraction procedures for PCR examination. Initially, all specimens were tested for presence of HPV virus followed by specific PCR testing for 16, 18, 13, and 32 subtypes in positive samples.
  
  
  RESULTS
  Human papilloma virus was found in all samples. In five cases HPV13 and in one case HPV32 was positive. One case showed strong reactivity for HPV but none of tested subtypes were positive. All cases were negative for HPV 16 and 18.
  
  
  CONCLUSIONS
  Similar with other studies about FEH, most of our cases were associated with HPV 13 but other subtypes may also be implicated.
authors.affiliation: Department of Oral Medicine, Faculty of Dentistry and Dental, Research Center, Mashhad University of Medical Sciences, P.O. Box 91735-984, Mashhad, Iran. farnazfalaki@yahoo.com
authors.forename: F
authors.initials: F
authors.lastname: Falaki
authors.forename: M
authors.initials: M
authors.lastname: Amir Chaghmaghi
authors.forename: A
authors.initials: A
authors.lastname: Pakfetrat
authors.forename: Z
authors.initials: Z
authors.lastname: Delavarian
authors.forename: P M
authors.initials: PM
authors.lastname: Mozaffari
authors.forename: N
authors.initials: N
authors.lastname: Pazooki
mesh_terms: D000293:Adolescent; D000328:Adult; D002648:Child; D004279:DNA, Viral / Q000032:analysis*; D005260:Female; D017573:Focal Epithelial Hyperplasia / Q000473:pathology / Q000821:virology*; D005838:Genotype; D052162:Human papillomavirus 16 / Q000235:genetics; D052161:Human papillomavirus 18 / Q000235:genetics; D006801:Humans; D007492:Iran; D008297:Male; D027383:Papillomaviridae / Q000145:classification* / Q000235:genetics; D030361:Papillomavirus Infections / Q000473:pathology / Q000821:virology*; D016133:Polymerase Chain Reaction
chemical_list: D004279:DNA, Viral