[Studies on detection of E. coli O157:H7 by ATP bioluminescence].
In the present paper, a quantitative linear model betweena series of concentrations of E. coliO157 : H7 and counts by BPCL ultra weak luminescence analyzer was built up. And the influences of four different buffers with the same pH (pH = 7.4), Tris-HCl, PBS, KH2PO4-NaOH and Na2 HPO4-C6H8O7, and five different chemical substances with the same mass concentration (10 g x L), NaCl, KCl, NaOH, MgCl2 and NaH2PO4 on ATP bioluminescence were compared. The results showed that Tris-HCl was a suitable buffer for dilution, since it could distinguish well between different concentrations and had the lowest background signals. And MgCl2 could intensify luminescence distinctly, while the other four chemical substances decreased luminescence, of which NaOH decreased luminescence most obviously. Moreover, ATP bioluminescence was correlated well with conventional culture methods (r = 0.96), and the detection limit was 10(3) cells x mL(-1).
Tang
Qian-Qian
QQ
College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310029, China. tangqq252@163.com
Wang
Jian-Ping
JP
Gai
Lin
L
Ye
Zun-Zhong
ZZ
Ying
Yi-Bin
YB
D000255:Adenosine Triphosphate / Q000737:chemistry*; D002021:Buffers; D019453:Escherichia coli O157 / Q000302:isolation & purification*; D016014:Linear Models; D008163:Luminescent Measurements / Q000379:methods*; D012492:Salts / Q000737:chemistry
D002021:Buffers; D012492:Salts; D000255:Adenosine Triphosphate