title: Rapid PFGE method for fingerprinting of Serratia marcescens isolates.
abstract: We modified the conventional PFGE procedure for Serratia marcescens to establish a rapid method. Our protocol showed modification in the bacterial lysis, washing, and restriction enzyme digestion time. This resulted in shortening the time needed by about 3 days compared to the conventional PFGE method.
authors.affiliation: Department of Biological and Medical Research, King Faisal Specialist Hospital and Research Centre, Riyadh, Saudi Arabia. alaidan@kfshrc.edu.sa
authors.forename: Alwaleed
authors.initials: A
authors.lastname: Alaidan
authors.forename: Marie Fe
authors.initials: MF
authors.lastname: Bohol
authors.forename: Sahar I
authors.initials: SI
authors.lastname: Al-Thawadi
authors.forename: Abdulrahman
authors.initials: A
authors.lastname: Al-Suwaine
authors.forename: Ahmed A
authors.initials: AA
authors.lastname: Al-Qahtani
authors.forename: Mohammed N
authors.initials: MN
authors.lastname: Al-Ahdal
mesh_terms: D015373:Bacterial Typing Techniques / Q000379:methods*; D016172:DNA Fingerprinting / Q000379:methods*; D004269:DNA, Bacterial / Q000235:genetics; D016521:Electrophoresis, Gel, Pulsed-Field / Q000379:methods*; D005838:Genotype; D017720:Molecular Epidemiology / Q000379:methods; D012706:Serratia marcescens / Q000145:classification* / Q000235:genetics*; D013997:Time Factors
chemical_list: D004269:DNA, Bacterial