[Primry research of separation and culture of adult hepatocytes].
OBJECTIVE
To explore the separation and culture method of adult hepatocytes.


METHODS
The isolated adult hepatocytes were cultivated by RPMI 1640 medium at 37 degrees C in vitro. The characteristics of the growing hepatocytes were observed. Their synthesis of urea was detected. The transformation efficiency and density's change of lidocaine were analyzed.


RESULTS
Hepatocytes were successful separated from adult liver. And they were cultivated in common condition and hollow fiber reactor. The functional capacity of hepatocytes was for lidocaine metabolism and urea excretion.


CONCLUSIONS
The adult hepatocytes have been successful separated from liver. And they can be cultivated in common condition and hollow fiber reactor. And it could provide a great quantity and high activity of hepatocytes for bioartificial liver.
Liu
Hong-ling
HL
Department of Virology, Institute of Infectious Diseases, No. 302 Hospital, Beijing 100039, China.
Xin
Shao-jie
SJ
You
Shao-li
SL
Hu
Yan
Y
Rong
Yi-hui
YH
Zhou
Lin
L
Yang
Yong-ping
YP
Li
Zhi-wei
ZW
Liu
Zhen-wen
ZW
Mao
Pan-yong
PY
D000818:Animals; D018929:Cell Culture Techniques; D002478:Cells, Cultured; D022781:Hepatocytes / Q000378:metabolism*; D006801:Humans; D008099:Liver / Q000166:cytology*; D019164:Liver, Artificial / Q000639:trends*; D012106:Research