Fabrication of a molecularly imprinted polymer sensor by self-assembling monolayer/mediator system.
Detection of dopamine (DA) by using a molecularly imprinted polymer (MIP) which fabricated by the self-assembling monolayer (SAM)/mediator system was studied. The SAM was made by attaching thioglycolic acid (TGA) on a gold electrode and quercetin (Q) was selected as an electron transfer mediator in this system. Methyl methacrylate (MMA) was polymerized by photopolymerization with the addition of dopamine to form a MIP electrode. The MIP and non-MIP (NMIP) modified electrodes were identified by FTIR and scanning electrochemical microscopy (SECM) approach curves. DA was detected by an amperometric method at a constant potential of 0.45 V and obtained a sensitivity of 0.445 mA cm(-2) M(-1). The imprinting efficiency approaches infinity due to a non-reactive surface of NMIP. In the interference test, ascorbic acid contributed less than 12% of current response in the coexistence solution with DA and the performance is highly related to the concentration of template added during the fabrication process.
Chen
Po-Yen
PY
Department of Chemical Engineering, National Taiwan University, Taipei 10617, Taiwan.
Nien
Po-Chin
PC
Wu
Cheng-Tar
CT
Wu
Tsing-Hua
TH
Lin
Chii-Wann
CW
Ho
Kuo-Chuan
KC
D004298:Dopamine / Q000032:analysis; D004566:Electrodes; D004583:Electrons; D006046:Gold / Q000737:chemistry*; D054802:Molecular Imprinting; D019904:Polymethyl Methacrylate / Q000138:chemical synthesis / Q000737:chemistry*; D011794:Quercetin / Q000737:chemistry*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D052616:Solid Phase Extraction; D013499:Surface Properties; D013864:Thioglycolates / Q000737:chemistry*; D013997:Time Factors
D013864:Thioglycolates; D006046:Gold; D019904:Polymethyl Methacrylate; D011794:Quercetin; D004298:Dopamine