Isolation and immunomodulatory effect of homoisoflavones and flavones from Agave sisalana Perrine ex Engelm.
Three known flavones and seven known homoisoflavonoids were isolated from the methanolic extract of the leaves of Agave sisalanaPerrine ex Engelm. Their structures were elucidated on the basis of spectroscopic analysis. The isolated compounds were also evaluated for immunopharmacological activity. PBMC were used as target cells, and cell proliferation was determined by (3)H-thymidine uptake. (+/-)-3,9-Dihydroeucomin (4), dihydrobonducellin (5), and 5,7-dihydroxy-3-(4'-hydroxybenzyl)-4-chromanone (7) showed inhibitory effects on PBMC proliferation activated by PHA with IC(50) values 19.4, 73.8, and 58.8 microM, respectively. All three compounds significantly inhibited the production of IL-2 and IFN-gamma in activated PBMC in a concentration-dependent manner.
Chen
Pi Yu
PY
Graduate Institute of Pharmacy, Taipei Medical University, Taipei, Taiwan.e023089103@tmu.edu.tw
Kuo
Yuh Chi
YC
Chen
Chin Hui
CH
Kuo
Yueh Hsiung
YH
Lee
Ching Kuo
CK
D027882:Agave / Q000033:anatomy & histology / Q000737:chemistry* / Q000276:immunology; D047309:Flavones / Q000276:immunology / Q000302:isolation & purification; D007155:Immunologic Factors / Q000276:immunology / Q000302:isolation & purification; D007529:Isoflavones / Q000276:immunology / Q000302:isolation & purification; D015394:Molecular Structure; D010936:Plant Extracts / Q000276:immunology / Q000302:isolation & purification; D018515:Plant Leaves / Q000737:chemistry
D047309:Flavones; D007155:Immunologic Factors; D007529:Isoflavones; D010936:Plant Extracts