title: Histologic features of transplanted amniotic membrane: implications for corneal wound healing.
abstract: OBJECTIVE
  To evaluate the histologic changes occurring in the transplanted amniotic membrane in human eyes.
  
  
  METHODS
  Observational consecutive case series.
  
  
  METHODS
  Seven consecutive patients who underwent amniotic membrane transplantation (AMT) for bullous keratopathy and subsequently had a penetrating keratoplasty (PK).
  
  
  METHODS
  Corneal buttons obtained at PK were examined by light and electron microscopy and by immunohistology with antibodies against CD34 (keratocytes), alpha smooth muscle actin and vimentin (myofibroblasts and fibroblasts respectively). Time from AMT to PK ranged from 2 to 32 months.
  
  
  METHODS
  Immunophenotypic characteristics of cells populating transplanted amniotic stroma.
  
  
  RESULTS
  Amniotic tissue was covered with stratified corneal epithelium with well-defined desmosomes and hemidesmosomes. Transformed corneal stroma-derived cells (CSDCs) could be seen migrating from the anterior stroma, through breaks in the Bowman's zone, into connective tissue of the amniotic membrane. Immunohistology showed that the cells populating amniotic stroma were CD34 negative but positive for vimentin and alpha smooth muscle actin. In 2 samples in which corneal transplants were performed approximately 1 year or more after AMT, some cells in the amniotic stroma showed CD34+ staining. Features of increased metabolic activity and formation of new collagen were seen on electron microscopy. In 2 cases, epithelial cell nests were seen in the amniotic stroma.
  
  
  CONCLUSIONS
  The amniotic basement membrane facilitates epithelial cell migration and adhesion. The amniotic stroma supports CSDCs and epithelial cells. Repopulation of the amniotic stroma by CSDCs migrating through breaks in Bowman's zone integrates the amnion with corneal tissue and allows for rebuilding of corneal stroma. Over time, some CSDCs may revert to the resting keratocyte immunophenotype.
authors.affiliation: Division of Ophthalmology, University of Nottingham, University Hospital, Queens Medical Centre, Nottingham, England.
authors.forename: Dalia G
authors.initials: DG
authors.lastname: Said
authors.forename: Mario
authors.initials: M
authors.lastname: Nubile
authors.forename: Thaer
authors.initials: T
authors.lastname: Alomar
authors.forename: Andy
authors.initials: A
authors.lastname: Hopkinson
authors.forename: Trevor
authors.initials: T
authors.lastname: Gray
authors.forename: James
authors.initials: J
authors.lastname: Lowe
authors.forename: Harminder S
authors.initials: HS
authors.lastname: Dua
mesh_terms: D000199:Actins / Q000378:metabolism; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000650:Amnion / Q000378:metabolism / Q000473:pathology* / Q000637:transplantation*; D018952:Antigens, CD34 / Q000378:metabolism; D002448:Cell Adhesion / Q000502:physiology; D002465:Cell Movement / Q000502:physiology; D003316:Corneal Diseases / Q000601:surgery*; D003319:Corneal Stroma / Q000378:metabolism / Q000648:ultrastructure*; D019573:Epithelium, Corneal / Q000378:metabolism / Q000648:ultrastructure*; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D016130:Immunophenotyping; D015948:Keratoplasty, Penetrating; D008297:Male; D008875:Middle Aged; D014746:Vimentin / Q000378:metabolism; D014945:Wound Healing
chemical_list: D000199:Actins; D018952:Antigens, CD34; D014746:Vimentin