[Determination of common antibiotics and metronidazole in cosmetics by ultra performance liquid chromatography-tandem mass spectrometry].
A method for the analysis of common antibiotics and metronidazole in cosmetics was developed by ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/ MS). One gram of each cosmetic sample was extracted by methanol-water containing 0.1 mol/L formic acid (1:1, v/v). The extracted sample was filtered before being analyzed with UPLC/MS/MS. The effects of formic acid concentration in the mobile phase on the sensitivity and retention time were studied, and the results showed that 1% was the optimum concentration. Seven analytes, minocycline, oxytetracycline, tetracycline, chlortetracycline, doxycycline, chloramphenicol, metronidazole, can be separated and detected in 5 min, which is much faster than that by the conventional liquid chromatography. The detection limits were 3 - 20 ng/g, and the recoveries were 87% -101%. The calibration curves showed good linearity in the range of 2 - 1 000 microg/L with correlation coefficients larger than 0.995. The method was also applied to determine common antibiotics and metronidazole in 11 real samples from the market. Chloramphenicol was detected in 2 samples and the concentrations were 0.37% and 0.19%; metronidazole was detected in 1 sample and the concentration was 1.02%; others were not detected.
Liu
Hualiang
H
Jiangsu Provincial Center for Disease Control and Prevention, Nanjing 210009, China. lhl-1@163.com
Li
Fang
F
Yang
Run
R
Wang
Lianhong
L
Ma
Yongji
Y
D000900:Anti-Bacterial Agents / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003257:Consumer Product Safety; D003358:Cosmetics / Q000032:analysis*; D008795:Metronidazole / Q000032:analysis*; D053719:Tandem Mass Spectrometry / Q000379:methods*
D000900:Anti-Bacterial Agents; D003358:Cosmetics; D008795:Metronidazole