title: An old method for good new cells.
abstract: The aim of this work was to demonstrate a greater number of viable cells using a micro-surgical in-situ perfusion to collect rat pancreata compared with the pancreas after exsanguination. We used 3 groups of 20 rats. Perfusion was performed by selective cannulation of the left common iliac artery with administration of UW solution at 4 degrees C. Collected pancreata were digested and cells separated by Ficoll gradient were placed in culture to permit adhesion to dishes. Cells were characterized and tested for viability. We observed a gain of about 14% in the number of viable cells compared with those obtained after exsanguination (P < .001 by chi-square).
authors.affiliation: University of Palermo School of Medicine, Palermo, Italy.
authors.forename: G
authors.initials: G
authors.lastname: Damiano
authors.forename: M C
authors.initials: MC
authors.lastname: Gioviale
authors.forename: C
authors.initials: C
authors.lastname: Lombardo
authors.forename: A I
authors.initials: AI
authors.lastname: Lo Monte
mesh_terms: D000241:Adenosine; D000493:Allopurinol; D000818:Animals; D018941:Antigens, CD19 / Q000378:metabolism; D001012:Aorta, Abdominal; D002470:Cell Survival; D005260:Female; D005362:Ficoll; D005978:Glutathione; D007328:Insulin; D008297:Male; D019188:Organ Preservation Solutions; D010179:Pancreas / Q000166:cytology; D010477:Perfusion / Q000379:methods*; D011887:Raffinose; D017208:Rats, Wistar
chemical_list: D018941:Antigens, CD19; D007328:Insulin; D019188:Organ Preservation Solutions; C055206:University of Wisconsin-lactobionate solution; D005362:Ficoll; D000493:Allopurinol; D005978:Glutathione; D000241:Adenosine; D011887:Raffinose