[An experimental study on recombinant adenovirus p53 transfected in oral dysplastic epithelial cells].
OBJECTIVE
To investigate and evaluate the appropriate virus titer and transfection efficiency of recombinant adenovirus p53 into the oral dysplastic epithelial cells (POE-9n) and provide reference for oral precancerosis research.


METHODS
The transfection sensitivity of adenovirus into oral dysplastic epithelial cells was evaluated by the recombinant adenovirus p53 containing green fluorescent protein (rAd-GFP). Different titre rAd -p53 was transfected into oral dysplastic epithelial cells to evaluate the effects of rAd-p53 on cell proliferation inhibition by MIT assay. The expression of exogenous p53 gene in POE-9n cells was detected by immunocytochemistry.


RESULTS
More than 95% POE-9n cells were transfected by rAd-GFP with MOI from 100 to 500 and there was no statistical difference between different MOI values (r=-0.124, P>0.05). It was found that rAd-p53 had significant inhibition effects on POE-9n cell proliferation with MOI from 100 to 500, and there were no significant differences at 96 h and 120 h after the transfection on cell proliferation inhibition (P>0.05). P53 protein was well expressed in rAd-p53 transfected POE-9n cells.


CONCLUSIONS
Exogenous p53 can be successfully transfected into POE-9n cells by rAd-p53 and the virus titer of MOI 100 was high enough to ensure efficient transfection.
Xu
Bo
B
State Key Laboratory of Oral Diseases, Sichuan University, Chengdu 610041, China.
Zhang
Song-Tao
ST
Li
Long-Jiang
LJ
Han
Bo
B
Zhao
Hong-Wei
HW
Pan
Jian
J
D000256:Adenoviridae; D049109:Cell Proliferation; D004847:Epithelial Cells; D016158:Genes, p53; D005822:Genetic Vectors; D049452:Green Fluorescent Proteins; D006801:Humans; D014162:Transfection
D049452:Green Fluorescent Proteins