title: A novel biosensor based on photoelectro-synergistic catalysis for flow-injection analysis system/amperometric detection of organophosphorous pesticides.
abstract: In this study, a highly sensitive amperometric biosensor based on photoelectro-synergistic catalysis for detecting organophosphorus pesticides (OPs) in flow-injection analysis (FIA) system has been developed. The acetylcholinesterase enzyme (AChE) was immobilized by adsorption into the nanostructured PbO2/TiO2/Ti, which also acted as the working electrode. This strategy was found to catalyze the oxidative reaction of thiocholine effectively, make the AChE/PbO2/TiO2/Ti biosensor detect the substrate at 0.30 V (vs. SCE), hundreds milli-volts lower than others reported. PbO2/TiO2/Ti and TiO2/Ti electrodes were prepared and investigated with atomic force microscope (AFM). Factors influencing the performance were optimized. The resulting flow system offered a fast, sensitive, and stable response. A value of 1.34 mM for the apparent Michaelis-Menten constant (K(M)(app)) was obtained. A wide linear inhibition response for trichlorfon was observed in the range of 0.01-20 microM with the detection limit of 0.1 nM. The results using this biosensor agreed very well with chromatographic method and we also examined the real samples successfully in this work.
authors.affiliation: Department of Chemistry, East China Normal University, 3663 Zhong Shan Road North, Shanghai 200062, China.
authors.forename: Yinyin
authors.initials: Y
authors.lastname: Wei
authors.forename: Ying
authors.initials: Y
authors.lastname: Li
authors.forename: Yunhe
authors.initials: Y
authors.lastname: Qu
authors.forename: Fei
authors.initials: F
authors.lastname: Xiao
authors.forename: Guoyue
authors.initials: G
authors.lastname: Shi
authors.forename: Litong
authors.initials: L
authors.lastname: Jin
mesh_terms: D000110:Acetylcholinesterase / Q000378:metabolism; D000327:Adsorption; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D002384:Catalysis; D004563:Electrochemistry; D004566:Electrodes; D004800:Enzymes, Immobilized / Q000378:metabolism; D017022:Flow Injection Analysis / Q000295:instrumentation / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D007854:Lead / Q000737:chemistry; D018625:Microscopy, Atomic Force; D049329:Nanostructures / Q000737:chemistry; D009943:Organophosphorus Compounds / Q000032:analysis*; D010087:Oxides / Q000737:chemistry; D010316:Particle Size; D010575:Pesticides / Q000032:analysis*; D010777:Photochemistry; D012680:Sensitivity and Specificity; D013997:Time Factors; D014025:Titanium / Q000737:chemistry
chemical_list: D004800:Enzymes, Immobilized; D009943:Organophosphorus Compounds; D010087:Oxides; D010575:Pesticides; C009495:titanium dioxide; D007854:Lead; C047365:lead oxide; D014025:Titanium; D000110:Acetylcholinesterase