title: Isolation, growth and differentiation of equine mesenchymal stem cells: effect of donor, source, amount of tissue and supplementation with basic fibroblast growth factor.
abstract: Mesenchymal stem cells (MSC) are increasingly used as therapeutical aid for the orthopaedic injuries in the horse. MSC populate different tissues but the most commonly used for clinical purposes are isolated from bone marrow or adipose tissue. The first objective of this study was to investigate if the donor animal, the tissue of origin and the technique of isolation could influence the number of MSC available for transplantation after a short-term expansion. The second aim was to devise a culture system capable of increasing MSC lifespan and we tested the effect of basic fibroblast growth factor (bFGF). Results indicate that MSC can be efficiently isolated from both sources and supplementation of bFGF enhances proliferation rate maintaining differentiation potential. In addition, this study shows that collection, expansion and storage of frozen MSC can be performed for later therapeutic use.
authors.affiliation: Laboratorio di Tecnologie della Riproduzione, Avantea srl, Via Porcellasco 7/f, 26100 Cremona, Italy. silviacolleoni@avantea.it
authors.forename: Silvia
authors.initials: S
authors.lastname: Colleoni
authors.forename: Emanuela
authors.initials: E
authors.lastname: Bottani
authors.forename: Irene
authors.initials: I
authors.lastname: Tessaro
authors.forename: Gaetano
authors.initials: G
authors.lastname: Mari
authors.forename: Barbara
authors.initials: B
authors.lastname: Merlo
authors.forename: Noemi
authors.initials: N
authors.lastname: Romagnoli
authors.forename: Alessandro
authors.initials: A
authors.lastname: Spadari
authors.forename: Cesare
authors.initials: C
authors.lastname: Galli
authors.forename: Giovanna
authors.initials: G
authors.lastname: Lazzari
mesh_terms: D000273:Adipose Tissue / Q000166:cytology; D000818:Animals; D001854:Bone Marrow Cells / Q000502:physiology; D002454:Cell Differentiation / Q000502:physiology; D049109:Cell Proliferation; D002478:Cells, Cultured; D016222:Fibroblast Growth Factor 2 / Q000502:physiology*; D006736:Horses; D045164:Mesenchymal Stem Cell Transplantation / Q000662:veterinary; D059630:Mesenchymal Stem Cells / Q000502:physiology*
chemical_list: D016222:Fibroblast Growth Factor 2