[Evaluation of Amberlite XAD-1600 resin-based purification of the bioactive components from Radix Paeoniae Alba and Rhizoma Curcumae Longae extracts by pharmacodynamic and toxicological models].
OBJECTIVE
To evaluate the pharmacodynamics and toxicicity of the major bioactive components extracted and purified from Radix Paeoniae Alba and Rhizoma Curcumae Longae using Amberlite XAD-1600 resin.


METHODS
Amberlite XAD-1600 was used to purify the bioactive components from the crude 75% ethanol extracts of the two herbs. The pharmacodynamic and toxic effects of the crude extracts and extract purified using XAD-1600 resin were comparatively examined with two acute inflammatory models, two pain models and acute toxicity test in vivo.


RESULTS
The anti-inflammatory and analgesic effects of the purified extract were significant stronger with lower toxicity than those of the crude ethanol extract.


CONCLUSIONS
Amberlite XAD-1600 resin allows efficient extraction and purification of the bioactive components from the two herbs.
Wu
Zhi-hua
ZH
School of Pharmaceutical Sciences, Southern Medical University, Guangzhou 510515, China. wykgz@126.com
Liu
Zhong-qiu
ZQ
Fang
Yan-fang
YF
Zhang
Jia-jie
JJ
D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000302:isolation & purification* / Q000494:pharmacology; D030024:Curcuma / Q000737:chemistry*; D004365:Drugs, Chinese Herbal / Q000302:isolation & purification* / Q000494:pharmacology / Q000633:toxicity; D005260:Female; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D029596:Paeonia / Q000737:chemistry*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012117:Resins, Synthetic / Q000737:chemistry*
D000894:Anti-Inflammatory Agents, Non-Steroidal; D004365:Drugs, Chinese Herbal; D012117:Resins, Synthetic; C046797:amberlite