Aberrant expression of serum amyloid A in head and neck squamous cell carcinoma.
Serum amyloid A (SAA) is an acute-phase reactant, the blood level of which is often elevated in response to some types of neoplasia. Here, we investigated expression of the gene SAA1 and the protein SAA in head and neck squamous cell carcinoma (HNSCC) and normal oral mucosal tissues as well as blood SAA levels in HNSCC patients. Also, we investigated the effects of inhibiting signal transducer and activator of transcription 3 (STAT3) signaling on SAA1 expression in the HNSCC cell line SAS. Serum SAA levels in HNSCC patients were significantly higher than those in healthy volunteers. In addition, real-time quantitative reverse transcription-polymerase chain reaction analysis revealed a significantly higher SAA1 expression level in HNSCC than in normal mucosa (P < 0.0001). Furthermore, Western blot and immunohistochemical analyzes revealed that high expression of SAA in carcinomas was detected predominantly in tumor cells, but not in normal mucosal tissues. An inhibitor of STAT3 activation, AG490, significantly reduced SAA1 expression in SAS cells. These data demonstrated that SAA was up-regulated in HNSCC through the Janus kinase-STAT3 pathway.
Shinriki
Satoru
S
Department of Oral and Maxillofacial Surgery, Graduate School of Medical Sciences, Kumamoto University, Kumamoto.
Ueda
Mitsuharu
M
Ota
Kazutoshi
K
Nakamura
Masaaki
M
Kudo
Mareina
M
Ibusuki
Mutsuko
M
Kim
Jaemi
J
Yoshitake
Yoshihiro
Y
Fukuma
Daiki
D
Jono
Hirofumi
H
Kuratsu
Jun-Ichi
J
Shinohara
Masanori
M
Ando
Yukio
Y
D000368:Aged; D015153:Blotting, Western; D002294:Carcinoma, Squamous Cell / Q000097:blood / Q000473:pathology*; D045744:Cell Line, Tumor; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005260:Female; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics; D005881:Gingiva / Q000473:pathology; D006258:Head and Neck Neoplasms / Q000097:blood / Q000473:pathology*; D006801:Humans; D007150:Immunohistochemistry; D053612:Janus Kinases / Q000187:drug effects; D008297:Male; D009061:Mouth Mucosa / Q000473:pathology; D009367:Neoplasm Staging; D020133:Reverse Transcriptase Polymerase Chain Reaction; D050796:STAT3 Transcription Factor / Q000032:analysis / Q000037:antagonists & inhibitors / Q000235:genetics; D000685:Serum Amyloid A Protein / Q000032:analysis* / Q000037:antagonists & inhibitors / Q000235:genetics; D020032:Tyrphostins / Q000494:pharmacology; D015854:Up-Regulation / Q000187:drug effects
D004791:Enzyme Inhibitors; C521404:SAA1 protein, human; D050796:STAT3 Transcription Factor; C494086:STAT3 protein, human; D000685:Serum Amyloid A Protein; D020032:Tyrphostins; C095512:alpha-cyano-(3,4-dihydroxy)-N-benzylcinnamide; D053612:Janus Kinases