Investigation of PON1 192 and PON1 55 polymorphisms in ovarian cancer patients in Turkish population.
BACKGROUND
Ovarian cancer is the leading cause of death due to gynecological malignancies among women. Oxidative stress is potentially harmful to cells and reactive oxygen species are known to be involved in the initiation and progression of cancer. Paraoxonase (PON1) is an antioxidative enzyme, which eliminates lipid peroxides. PON1 has two common polymorphisms (M/L55 and A/B192) that influence PON1 concentration and activity.


METHODS
Whether or not the M/L55 or A/B192 genotype relates with ovarian cancer was studied in 51 patients and 54 controls. Polymerase chain reaction (PCR) restriction fragment length polymorphism (RFLP), and agarose gel electrophoresis techniques were used to determine these polymorphisms.


RESULTS
The proportion of smokers was significantly higher in the patients than the controls (26.9% vs. 7%; Chi-square: 7.81, p:0.005; Odds ratio (OR): 4.88 95% CI: 1.49-15.99). The frequencies of the PON1 192 AA, BB and AB genotypes among the patients were 0.76, 0.12 and 0.12 and among the control subjects, 0.33, 0.11 and 0.56, respectively. The AA genotype frequency was significantly higher in the patients than the controls (Chi-square: 19.242, p=0.000; OR: 2.80 95% CI:1.653-4.757). The frequencies of the PON1 55 LL, MM and LM genotypes among the patients were 0.53, 0.10 and 0.37 and among the control subjects there were 0.46, 0.04 and 0.50, respectively. The MM genotype frequency was higher in the patients than the controls, but not statistically significantly (p>0.05).


CONCLUSIONS
The two polymorphisms were associated with the age of onset of ovarian cancer, which increased in the genotype order AB<AA<BB in the PON1 192 polymorphism and LM<LL<MM in the PON1 55 polymorphism. The PON1 192 AA genotype may play an important role as a risk factor for ovarian cancer in the Turkish population and the A and L alleles may be associated with early onset of disease.
Arpaci
Aytaç
A
Department of Molecular Medicine, Istanbul University, Capa, Istanbul, Turkey.
Görmüs
Uzay
U
Dalan
Burak
B
Berkman
Sinan
S
Isbir
Turgay
T
D043303:Aryldialkylphosphatase / Q000235:genetics*; D001483:Base Sequence; D017931:DNA Primers; D005260:Female; D006801:Humans; D010051:Ovarian Neoplasms / Q000235:genetics*; D011110:Polymorphism, Genetic; D014421:Turkey
D017931:DNA Primers; D043303:Aryldialkylphosphatase; C468406:PON1 protein, human