[Immunoscreening and identification of Schistosoma japonicum juvenile cDNA library].
The cDNA library of Schistosoma japonicum (Sj) juveniles was immunoscreened with the anti-serum from day 14 post-infection mice. The inserts of the seven positive clones were sequenced and analyzed for their homology in GenBank database. Results showed that one was highly homologous to the SjHSP70 (score=650), two were significantly homologous to the SjFABP (score=229) and Sj CDGSH-type Zn finger-containing protein-like protein (score=246), and the other four were not homologous to genes in GenBank and thus identified as Sj novel genes. The sequences of the novel genes were submitted to GenBank and the accession numbers were obtained (EU121231, 202646, 202647 and 202648).
Duan
Xin-wei
XW
Biotechnology School, East China University of Science and Technology, Shanghai 200237, China.
Fu
Ying-hui
YH
Lu
Yan
Y
Huang
Cheng-yu
CY
Ju
Chuan
C
Xu
Bin
B
Xu
Xue-nian
XN
Feng
Zheng
Z
Hu
Wei
W
D000818:Animals; D000947:Antigens, Helminth / Q000235:genetics*; D018076:DNA, Complementary; D005260:Female; D015723:Gene Library; D017238:Genes, Helminth; D051379:Mice; D008815:Mice, Inbred Strains; D008969:Molecular Sequence Data; D012549:Schistosoma japonicum / Q000235:genetics* / Q000276:immunology*
D000947:Antigens, Helminth; D018076:DNA, Complementary