title: Elucidation of the regulation of an adult cuticle gene Acp65A by the transcription factor Broad.
abstract: Broad (BR), an ecdysone-inducible transcription factor, is a major determinant of the pupal stage. The misexpression of BR-Z1 isoform (BR-Z1) during adult development of Drosophila melanogaster prevents the expression of the adult cuticle protein 65A gene (Acp65A). We found that the proximal 237 bp of the 5' flanking region of Acp65A were sufficient to mediate this suppression. A targeted point mutation of a putative BR-Z1 response element (BRE) within this region showed that it was not involved. Drosophila hormone receptor-like 38 (DHR38) is required for Acp65A expression. We found that BR-Z1 repressed DHR38 expression and that BR's inhibition of Acp65A expression was rescued by exogenous expression of DHR38. Thus, BR-Z1 suppresses Acp65A expression by preventing the normal up-regulation of DHR38 at the time of adult cuticle formation.
authors.affiliation: Department of Biology, University of Washington, Seattle, WA, USA.
authors.forename: H-Y
authors.initials: HY
authors.lastname: Cui
authors.forename: M
authors.initials: M
authors.lastname: Lestradet
authors.forename: N
authors.initials: N
authors.lastname: Bruey-Sedano
authors.forename: J-P
authors.initials: JP
authors.lastname: Charles
authors.forename: L M
authors.initials: LM
authors.lastname: Riddiford
mesh_terms: D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism*; D004331:Drosophila melanogaster / Q000235:genetics* / Q000378:metabolism*; D018507:Gene Expression Regulation, Developmental / Q000502:physiology*; D006358:Hot Temperature; D019476:Insect Proteins / Q000235:genetics / Q000378:metabolism*; D034582:Integumentary System / Q000254:growth & development; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D011679:Pupa; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*
chemical_list: C102117:Br protein, Drosophila; D029721:Drosophila Proteins; D019476:Insect Proteins; D014157:Transcription Factors; C047663:cuticle proteins, insects