title: Heat-treated Lactobacillus crispatus KT strains reduce allergic symptoms in mice.
abstract: In the current study, we investigated the effects of heat-treated Lactobacillus crispatus KT strains on allergic response in mice. We found that the number of interferon (IFN)-gamma(+)CD4(+) cells was higher in C3H/HeN mouse spleen cultures incubated with L. crispatus KT strains than in those cultured with Lactobacillus JCM type cultures. The serum immunoglobulin E levels in NC/Nga mice that were administered KT strains were lower than those in the mice that were not given any bacterium. The ratio of spleen IFN-gamma(+)CD4(+)/interleukin-4(+)CD4(+) was highest in mice given L. crispatus KT-11. L. crispatus KT-11 also increased the expression of Toll-like receptor (TLR) 2, nucleotide-binding oligomerization domain (NOD) 1, and NOD2 in C3H/HeN mouse Peyer's patch cells. These results suggest that the L. crispatus KT-11 strain reduces allergic symptoms in NC/Nga mice via the adjustment of the type 1 helper T cell and type 2 helper T cell balance via TLR2, NOD1, and NOD2.
authors.affiliation: Interdisciplinary Graduate School of Science and Technology, Shinshu University, Kamiina-gun, Nagano, Japan.
authors.forename: Keisuke
authors.initials: K
authors.lastname: Tobita
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Yanaka
authors.forename: Hajime
authors.initials: H
authors.lastname: Otani
mesh_terms: D000818:Animals; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D002478:Cells, Cultured; D004195:Disease Models, Animal; D015870:Gene Expression; D006358:Hot Temperature; D006801:Humans; D006967:Hypersensitivity / Q000235:genetics / Q000276:immunology* / Q000382:microbiology; D007073:Immunoglobulin E / Q000097:blood; D007778:Lactobacillus / Q000276:immunology*; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D010581:Peyer's Patches / Q000276:immunology; D019936:Probiotics / Q000008:administration & dosage*; D013154:Spleen / Q000276:immunology*; D018417:Th1 Cells / Q000276:immunology; D018418:Th2 Cells / Q000276:immunology; D051195:Toll-Like Receptor 2 / Q000235:genetics / Q000276:immunology
chemical_list: D051195:Toll-Like Receptor 2; D007073:Immunoglobulin E