Corticosterone and dehydroepiandrosterone in songbird plasma and brain: effects of season and acute stress.
Prolonged increases in plasma glucocorticoids can exacerbate neurodegeneration. In rats, these neurodegenerative effects can be reduced by dehydroepiandrosterone (DHEA), an androgen precursor with anti-glucocorticoid actions. In song sparrows, season and acute restraint stress affect circulating levels of corticosterone and DHEA, and the effects of stress differ in plasma collected from the brachial and jugular veins. Jugular plasma is an indirect index of the neural steroidal milieu. Here, we directly measured corticosterone and DHEA in several brain regions and jugular plasma, and examined the effects of season and acute restraint stress (30 min) (n = 571 samples). Corticosterone levels were up to 10x lower in brain than in jugular plasma. In contrast, DHEA levels were up to 5x higher in brain than in jugular plasma and were highest in the hippocampus. Corticosterone and DHEA concentrations were strongly seasonally regulated in plasma but, surprisingly, not seasonally regulated in brain. Acute stress increased corticosterone levels in plasma and brain, except during the molt, when stress unexpectedly decreased corticosterone levels in the hippocampus. Acute stress increased DHEA levels in plasma during the molt but had no effects on DHEA levels in brain. This is the first study to measure (i) corticosterone or DHEA levels in the brain of adult songbirds and (ii) seasonal changes in corticosterone or DHEA levels in the brain of any species. These results highlight several critical differences between systemic and local steroid concentrations and the difficulty of using circulating steroid levels to infer local steroid levels within the brain.
Newman
Amy E M
AE
Department of Psychology, University of British Columbia, Vancouver, BC, Canada. newmana@interchange.ubc.ca
Soma
Kiran K
KK
D000311:Adrenal Glands / Q000378:metabolism; D000704:Analysis of Variance; D000818:Animals; D001921:Brain / Q000378:metabolism*; D003345:Corticosterone / Q000097:blood / Q000378:metabolism*; D003687:Dehydroepiandrosterone / Q000097:blood / Q000378:metabolism*; D006066:Gonads / Q000378:metabolism; D006624:Hippocampus / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D008297:Male; D018978:Molting / Q000502:physiology; D009132:Muscles / Q000378:metabolism; D012149:Restraint, Physical; D012621:Seasons; D046370:Sparrows / Q000097:blood / Q000378:metabolism*; D013315:Stress, Psychological / Q000097:blood / Q000378:metabolism
D003687:Dehydroepiandrosterone; D003345:Corticosterone