Comparison of effects of endogenous and exogenous excitatory amino acids on Retzius nerve cells of the leech.
In this paper we examine the effects of L-aspartate, L-glutamate, and beta-N-oxalylamino-L-alanine (Lathyrus toxin) on Retzius nerve cells of the leech Haemopis sanguisuga. The goal was to compare the electrophysiological effects of endogenous vs. exogenous amino acids, known as potent neurotoxins, through the mechanism ofexcitotoxicity. We used classical intracellular recordings on Retzius nerve cells in isolated ganglia of the leech, and plotted dose-response curves to compare potencies. Our results show that Lathyrus toxin is more than 200 times more potent in depolarizing the membrane potential on our model than L-aspartate and L-glutamate, which are approximately equipotent.
Lopicic
Srdjan
S
Institute for Pathological Physiology, Medical Faculty Belgrade, Belgrade, Serbia. slopicic@med.bg.ac.yu
Nedeljkov
Vladimir
V
Cemerikic
Dusan
D
Dudvarski
Zoran
Z
Pavlovic
Dragan
D
Cutura
Nedjo
N
D000596:Amino Acids / Q000494:pharmacology* / Q000502:physiology; D000599:Amino Acids, Diamino / Q000494:pharmacology; D000818:Animals; D001224:Aspartic Acid / Q000494:pharmacology; D018698:Glutamic Acid / Q000494:pharmacology; D066298:In Vitro Techniques; D007865:Leeches / Q000187:drug effects* / Q000502:physiology; D008564:Membrane Potentials / Q000187:drug effects; D009474:Neurons / Q000187:drug effects*; D009498:Neurotoxins / Q000494:pharmacology*
D000596:Amino Acids; D000599:Amino Acids, Diamino; D009498:Neurotoxins; C100126:oxalyldiaminopropionic acid; D001224:Aspartic Acid; D018698:Glutamic Acid