Isolation and characterization of surfactin produced by Bacillus polyfermenticus KJS-2.
Bacillus polyfermenticus KJS-2 (BP-KJS-2) was used to produce a lipopeptide-type surfactin. To accomplish this, a surfactin-producing BP-KJS-2 was fermented by soybeans. The surfactin was then purified by a procedure including ethanol treatment and preparative chromatography. Next, the biochemical structure of the purified surfactin was analyzed by electrospray ionization mass spectrometry (ESI-MS) and high-resolution ESI Q-Tof mass spectrometry (Q-Tof MS). In addition, the masses of the four peaks were determined to be 1007, 1021, 1035, and 1049 m/z revealing that the compound was mixture with quasi-molecular ions. Taken together, these findings indicated that the lipopeptide had a cyclic structure and amino acid composition of Gln-Leu-Leu-Leu-Val-Asp-Leu-Leu, and that the major lipopeptide product of BP-KJS-2 is the surfactin isoform. In addition, this lipopeptide showed strong antimicrobial activity against bacteria at the level of 0.05 mg/mL.
Kim
Kang Min
KM
Department of Smart Food and Drugs, Inje University, Gimhae, 621-749, Korea.
Lee
Jin Young
JY
Kim
Chun Kyu
CK
Kang
Jae Seon
JS
D000890:Anti-Infective Agents / Q000737:chemistry* / Q000302:isolation & purification* / Q000378:metabolism; D001407:Bacillus / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D052978:Disk Diffusion Antimicrobial Tests; D005285:Fermentation; D006090:Gram-Negative Bacteria / Q000187:drug effects / Q000254:growth & development; D006094:Gram-Positive Bacteria / Q000187:drug effects / Q000254:growth & development; D055666:Lipopeptides / Q000737:chemistry* / Q000302:isolation & purification* / Q000378:metabolism; D015394:Molecular Structure; D010456:Peptides, Cyclic / Q000737:chemistry* / Q000302:isolation & purification* / Q000378:metabolism; D013025:Glycine max; D021241:Spectrometry, Mass, Electrospray Ionization
D000890:Anti-Infective Agents; D055666:Lipopeptides; D010456:Peptides, Cyclic