title: Formation and identification of trimethylimidazole during tetramethylpyrazine production from glucose by Bacillus strains.
abstract: During 2,3,5,6-tetramethylpyrazine production from glucose by Bacillus strains, a novel product was detected and identified as 2,4,5-trimethylimidazole (TMI) by GC/MS. TMI appeared in the culture medium only after glucose had been depleted and then increased to 0.25-0.31 g l(-1) in 90-120 h. When the ammonium source was changed from (NH(4))(2)SO(4) to (NH(4))(2)HPO(4), only about one tenth of TMI was detected. Although the mechanistic events largely remain unclear, both microbial strains tested demonstrated similar dynamic processes of TMI production, suggesting that TMI formation is a genuine feature of Bacillus species.
authors.affiliation: Center for Bioengineering & Biotechnology, China University of Petroleum, Qingdao 266555, People's Republic of China.
authors.forename: Zijun
authors.initials: Z
authors.lastname: Xiao
authors.forename: Jian R
authors.initials: JR
authors.lastname: Lu
authors.forename: Cuiqing
authors.initials: C
authors.lastname: Ma
authors.forename: Ping
authors.initials: P
authors.lastname: Xu
mesh_terms: D000645:Ammonium Sulfate / Q000378:metabolism; D001407:Bacillus / Q000378:metabolism*; D003470:Culture Media / Q000737:chemistry; D008401:Gas Chromatography-Mass Spectrometry; D005947:Glucose / Q000378:metabolism*; D007093:Imidazoles / Q000378:metabolism*; D010710:Phosphates / Q000378:metabolism; D011719:Pyrazines / Q000378:metabolism*
chemical_list: D003470:Culture Media; D007093:Imidazoles; D010710:Phosphates; D011719:Pyrazines; C024788:ammonium phosphate; C041683:2,4,5-trimethylimidazole; D005947:Glucose; D000645:Ammonium Sulfate; C017953:tetramethylpyrazine