QTL-specific microarray gene expression analysis of wheat resistance to Fusarium head blight in Sumai-3 and two susceptible NILs.
Fusarium head blight, predominantly caused by Fusarium graminearum (Schwabe) in North America, is a destructive disease that poses a serious threat to wheat (Triticum aestivum L.) production around the world. cDNA microarrays consisting of wheat ESTs derived from a wheat - F. graminearum interaction suppressive subtractive hybridization library were used to investigate QTL-specific differential gene expression between the resistant Chinese cultivar Sumai-3 and two susceptible near isogenic lines (NILs) following inoculation with F. graminearum. Stringent conditions were employed to reduce the false discovery rate. A total of 25 wheat unigenes were found to express differentially in response to F. graminearum infection. Genes encoding pathogenesis-related (PR) proteins such as beta-1,3-glucanase (PR-2), wheatwins (PR-4), and thaumatin-like proteins (PR-5) showed a significant upregulation in genotypes having the Sumai-3 3BS region. For these three genes, the gene activity was significantly less in the genotype (NIL-3) lacking the Sumai-3 3BS segment. Significant upregulation of phenylalanine ammonia-lyase was detected only in the resistant Sumai-3, indicating the importance of both the 2AL and 3BS regions in the activation of effective defense responses to infection by F. graminearum. Differences in gene expression between the resistant Sumai-3 and the susceptible NILs were found to be mainly quantitative in nature.
Golkari
Saber
S
Cereal Research Centre, Agriculture and Agri-Food Canada, 195 Dafoe Road, Winnipeg, MB R3T 2M9, Canada.
Gilbert
Jeannie
J
Ban
Tomohiro
T
Procunier
J Douglas
JD
D015415:Biomarkers / Q000378:metabolism; D015152:Blotting, Northern; D005670:Fusarium / Q000502:physiology*; D020869:Gene Expression Profiling; D017343:Genes, Plant; D007377:Interleukin-3 / Q000037:antagonists & inhibitors / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis; D010935:Plant Diseases / Q000235:genetics* / Q000382:microbiology; D040641:Quantitative Trait Loci; D012334:RNA, Neoplasm / Q000378:metabolism; D014908:Triticum / Q000235:genetics* / Q000382:microbiology
D015415:Biomarkers; D007377:Interleukin-3; D012334:RNA, Neoplasm