Cytotoxic components of Pereskia bleo (Kunth) DC. (Cactaceae) leaves.
Dihydroactinidiolide (1) and a mixture of sterols [campesterol (2), stigmasterol (3) and beta-sitosterol (4)], together with the previously isolated individual compounds beta-sitosterol (4), 2,4-di-tert-butylphenol (5), alpha-tocopherol (6), phytol (7) were isolated from the active ethyl acetate fraction of Pereskia bleo (Kunth) DC. (Cactaceae) leaves. Cytotoxic activities of the above mentioned compounds against five human carcinoma cell lines, namely the human nasopharyngeal epidermoid carcinoma cell line (KB), human cervical carcinoma cell line (CasKi), human colon carcinoma cell line (HCT 116), human hormone-dependent breast carcinoma cell line (MCF7) and human lung carcinoma cell line (A549); and non-cancer human fibroblast cell line (MRC-5) were investigated. Compound 5 possessed very remarkable cytotoxic activity against KB cells, with an IC(50 )value of 0.81microg/mL. This is the first report on the cytotoxic activities of the compounds isolated from Pereskia bleo.
Malek
Sri Nurestri Abdul
SN
Institute of Biological Sciences, Faculty of Science, University of Malaya, Kuala Lumpur, Malaysia. srimalek@um.edu.my
Shin
Sim Kae
SK
Wahab
Norhanom Abdul
NA
Yaacob
Hashim
H
D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D029421:Cactaceae / Q000033:anatomy & histology / Q000737:chemistry*; D045744:Cell Line, Tumor / Q000187:drug effects*; D002784:Cholesterol / Q000031:analogs & derivatives / Q000494:pharmacology; D004354:Drug Screening Assays, Antitumor; D006801:Humans; D015394:Molecular Structure; D010636:Phenols / Q000494:pharmacology; D010836:Phytol / Q000494:pharmacology; D010840:Phytosterols / Q000494:pharmacology; D018515:Plant Leaves / Q000737:chemistry*; D012855:Sitosterols / Q000494:pharmacology; D013265:Stigmasterol / Q000494:pharmacology; D024502:alpha-Tocopherol / Q000494:pharmacology
D000972:Antineoplastic Agents, Phytogenic; D010636:Phenols; D010840:Phytosterols; D012855:Sitosterols; D010836:Phytol; C021273:campesterol; C025473:gamma-sitosterol; D002784:Cholesterol; D013265:Stigmasterol; C056559:2,4-di-tert-butylphenol; D024502:alpha-Tocopherol