title: [Study on relationship between hepatitis B virus DNA load and genotype with large envelope protein].
abstract: OBJECTIVE
  To explore the relation between hepatitis B virus DNA load and genotype with the level of large envelope protein.
  
  
  METHODS
  Serum HBV DNA was quantitively detected by using real time polymerase chain reaction (RT-PCR). The LHBs were detected by using enzyme linked immuno sorbent assay (ELISA) and HBV markers were detected by time differentiate immunofluorescence assay in 140 serum samples collected from chronic hepatitis B patients.The genotypes of HBV were identified by DNA sequencing; and analyze their relationship.
  
  
  RESULTS
  There was no significant difference between positive rate of LHBs and that of HBV DNA in HBeAg negative and positive group (P > 0.05); The HBV LHBs absorbency was markedly correlated with the HBV DNA load ( R2 = 0.9267). The difference of HBV LHBs absorbency between HBV genotype B and C was not significant.
  
  
  CONCLUSIONS
  The close correlation between HBV LHBs absorbence and HBV DNA load illustrated that he level of serum LHBs can be used to estimate the state of HBV replication; and there is no relationship between HBV LHBs absorbency and genotypes. So HBV LHBs may be used as a new serological marker to detect HBV replication.
authors.affiliation: Wenling Hospital, Wenzhou Medical College, Wenzhou 317500, China. raogf@163.com
authors.forename: Gao-feng
authors.initials: GF
authors.lastname: Rao
authors.forename: En-fu
authors.initials: EF
authors.lastname: Chen
authors.forename: Ming-he
authors.initials: MH
authors.lastname: Yan
authors.forename: Min-qiao
authors.initials: MQ
authors.lastname: Zheng
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D004279:DNA, Viral / Q000032:analysis* / Q000235:genetics / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005838:Genotype; D006509:Hepatitis B / Q000235:genetics / Q000821:virology; D006515:Hepatitis B virus / Q000737:chemistry / Q000235:genetics*; D006801:Humans; D008297:Male; D008875:Middle Aged; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014759:Viral Envelope Proteins / Q000737:chemistry / Q000235:genetics; D014771:Virion / Q000737:chemistry / Q000235:genetics*; D055815:Young Adult
chemical_list: D004279:DNA, Viral; D014759:Viral Envelope Proteins