A leukemic plasmacytoid dendritic cell line, PMDC05, with the ability to secrete IFN-alpha by stimulation via Toll-like receptors and present antigens to naïve T cells.
We established a plasmacytoid dendritic cell (pDC) line (PMDC05) from leukemia cells of pDC leukemia. PMDC05 cells were positive for CD4, CD56, CD33, HLA-DR, CD123 (IL-3Ralpha) and CD86 in the absence of lineage markers. mRNA of TLR1, TLR2, TLR4, TLR7 and TLR9 was clearly expressed and among these TLRs, TLR7 was prominent. Production of IFN-alpha and IL-12 in PMDC05 was enhanced by the stimulation with CpG-A and LPS, respectively. PMDC05 possessed a considerable antigen presenting ability, which was enhanced by culturing with IL3, influenza virus or LPS. PMDC05 could be a useful tool for investigating the pathophysiology of pDCL.
Narita
Miwako
M
Laboratory of Hematology and Oncology, Graduate School of Health Sciences, Niigata University, Niigata, Japan. naritami@clg.niigata-u.ac.jp
Watanabe
Norihiro
N
Yamahira
Akie
A
Hashimoto
Shigeo
S
Tochiki
Nozomi
N
Saitoh
Anri
A
Kaji
Masami
M
Nakamura
Takeshi
T
Furukawa
Tatsuo
T
Toba
Ken
K
Fuse
Ichiro
I
Aizawa
Yoshifusa
Y
Takahashi
Masuhiro
M
D000941:Antigens / Q000276:immunology; D001483:Base Sequence; D045744:Cell Line, Tumor; D017931:DNA Primers; D003713:Dendritic Cells / Q000276:immunology / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D016898:Interferon-alpha / Q000378:metabolism*; D007621:Karyotyping; D007952:Leukemia, Plasma Cell / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013601:T-Lymphocytes / Q000276:immunology*; D051193:Toll-Like Receptors / Q000502:physiology*
D000941:Antigens; D017931:DNA Primers; D016898:Interferon-alpha; D051193:Toll-Like Receptors