title: Antimicrobial activity of calcium hydroxide pastes on Enterococcus faecalis cultivated in root canal systems.
abstract: The effectiveness of calcium hydroxide pastes: Calen and PMCC-Calen associated to chemo-mechanical preparation was assessed on Enterococcus faecalis grown within root canals. Seventy incisors were inserted into TSB medium, sterilized and contaminated with E. faecalis. Culture medium was replaced each 24 h and incubated at 37 degrees C for 72 h. After chemo-mechanical preparation, root canals were filled with Calen or PMCC-Calen (7 or 14 days). Pastes were removed and teeth were inserted into test tubes containing Enterococcosel broth. Calen paste (maintained for 7 and 14 days) induced 70% elimination of enterococci and PMCC-Calen 100% elimination only after maintenance for 14 days. These medications were significantly more effective (p<0.001) than chemo-mechanical protocol alone and PMCC-Calen maintained for 7 days, both incapable to eliminate the viability of enterococci. Calcium hydroxide pastes demonstrated important adjuvant effects in the elimination of enterococci during chemo-mechanical preparation of root canal systems. When associated with PMCC, calcium hydroxide pastes should be maintained for at least 14 days.
authors.affiliation: Department of Endodontics, Fluminense Federal University, Niterói, RJ, Brazil.
authors.forename: Patricia Elaine Panicali
authors.initials: PE
authors.lastname: Lana
authors.forename: Miriam Fatima Záccaro
authors.initials: MF
authors.lastname: Scelza
authors.forename: Licínio Esmeraldo
authors.initials: LE
authors.lastname: Silva
authors.forename: Ana Luíza de
authors.initials: AL
authors.lastname: Mattos-Guaraldi
authors.forename: Raphael
authors.initials: R
authors.lastname: Hirata Júnior
mesh_terms: D002126:Calcium Hydroxide / Q000494:pharmacology*; D002733:Chlorophenols / Q000494:pharmacology; D003786:Dental Pulp Cavity / Q000382:microbiology*; D004338:Drug Combinations; D013293:Enterococcus faecalis / Q000187:drug effects*; D006801:Humans; D007180:Incisor; D050296:Microbial Viability / Q000187:drug effects; D012387:Root Canal Filling Materials / Q000494:pharmacology*
chemical_list: D002733:Chlorophenols; D004338:Drug Combinations; D012387:Root Canal Filling Materials; C420643:calen; C029107:4-chlorophenol; D002126:Calcium Hydroxide