Expression of a family of noncoding mitochondrial RNAs distinguishes normal from cancer cells.
We reported the presence in human cells of a noncoding mitochondrial RNA that contains an inverted repeat (IR) of 815 nucleotides (nt) covalently linked to the 5' end of the mitochondrial 16S RNA (16S mtrRNA). The transcript contains a stem-loop structure and is expressed in human proliferating cells but not in resting cells. Here, we demonstrate that, in addition to this transcript, normal human proliferating cells in culture express 2 antisense mitochondrial transcripts. These transcripts also contain stem-loop structures but strikingly they are down-regulated in tumor cell lines and tumor cells present in 17 different tumor types. The differential expression of these transcripts distinguishes normal from tumor cells and might contribute a unique vision on cancer biology and diagnostics.
Burzio
Verónica A
VA
Andes Bioscience SA, Zañartu, Santiago, Chile. lburzio@bioschile.cl
Villota
Claudio
C
Villegas
Jaime
J
Landerer
Eduardo
E
Boccardo
Enrique
E
Villa
Luisa L
LL
Martínez
Ronny
R
Lopez
Constanza
C
Gaete
Fancy
F
Toro
Viviana
V
Rodriguez
Ximena
X
Burzio
Luis O
LO
D002460:Cell Line; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D002478:Cells, Cultured; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008928:Mitochondria / Q000235:genetics; D008969:Molecular Sequence Data; D009369:Neoplasms / Q000235:genetics*; D009690:Nucleic Acid Conformation; D012313:RNA / Q000737:chemistry / Q000235:genetics*; D016372:RNA, Antisense / Q000737:chemistry / Q000235:genetics*; D000077278:RNA, Mitochondrial; D022661:RNA, Untranslated / Q000737:chemistry / Q000235:genetics*
D016372:RNA, Antisense; D000077278:RNA, Mitochondrial; D022661:RNA, Untranslated; D012313:RNA