title: Molecular cloning, expression patterns and subcellular localization of porcine TMCO1 gene.
abstract: The product of transmembrane and coiled-coil domains 1 (TMCO1) gene is a member of DUF841 superfamily of several eukaryotic proteins with unknown function. The partial DNA sequence of porcine TMCO1 was first cloned with a pig 567 bp ORF encoding 188 amino acids. By tissues expression analysis, the TMCO1 was found highly expressed in the liver, kidney and heart. The porcine TMCO1 protein was subsequently demonstrated to localize in the mitochondrion by confocal fluorescence microscopy. This data provides an important basis for conducing further studies on the functions and regulatory mechanisms underlying the role of TMCO1 gene.
authors.affiliation: State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-Sen University, Guangzhou, People's Republic of China.
authors.forename: Zhisheng
authors.initials: Z
authors.lastname: Zhang
authors.forename: Delin
authors.initials: D
authors.lastname: Mo
authors.forename: Peiqing
authors.initials: P
authors.lastname: Cong
authors.forename: Zuyong
authors.initials: Z
authors.lastname: He
authors.forename: Fei
authors.initials: F
authors.lastname: Ling
authors.forename: Anning
authors.initials: A
authors.lastname: Li
authors.forename: Yuna
authors.initials: Y
authors.lastname: Niu
authors.forename: Xiao
authors.initials: X
authors.lastname: Zhao
authors.forename: Chunyan
authors.initials: C
authors.lastname: Zhou
authors.forename: Yaosheng
authors.initials: Y
authors.lastname: Chen
mesh_terms: D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D017931:DNA Primers / Q000235:genetics; D020869:Gene Expression Profiling; D007668:Kidney / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D008565:Membrane Proteins / Q000235:genetics* / Q000378:metabolism; D008856:Microscopy, Fluorescence; D024101:Mitochondrial Proteins / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D009206:Myocardium / Q000378:metabolism; D010957:Plasmids / Q000235:genetics; D017433:Protein Structure, Secondary; D040641:Quantitative Trait Loci; D017422:Sequence Analysis, DNA; D034421:Sus scrofa / Q000235:genetics* / Q000378:metabolism; D014162:Transfection
chemical_list: D017931:DNA Primers; D008565:Membrane Proteins; D024101:Mitochondrial Proteins