Effect of heme oxygenase-1 on the kidney during septic shock in rats.
The aim of this study was to clarify the effect of heme oxygenase (HO)-1 on the kidney during septic shock in rats. Eighty healthy and clean Sprague-Dawley rats were randomly divided into 4 groups: control group (group C) , septic shock group (group SS) , lipopolysaccharide plus ZnPP-IX group (group LZ) , and ZnPP-IX (group Z) . The plasma levels of COHb, Cr, and BUN; the urine levels of gamma-GTP, alpha1-MG, RBP, and NAG; and MDA content, SOD activity; HO-1mRNA; HO-2mRNA; HO-1 protein; and HO-2 protein from kidney were measured. The plasma levels of Cr and BUN; the urine levels of gamma-GTP, alpha1-MG, RBP, and NAG; and MDA content from kidney in group SS were obviously higher than in group C and Z (both P < 0.05), respectively, but lower than in group LZ (both P < 0.05), respectively. In contrast, the plasma levels of COHb and SOD activity from kidney in group SS were obviously lower than those in group C and Z (both P < 0.05), respectively, but higher than in group LZ (both P < 0.05), respectively. The HO-1mRNA and HO-1 protein of nephridial tissue in group LZ were obviously higher than in group C and Z, respectively (both P < 0.05), but lower than in group SS, respectively (both P < 0.05). There were no significant differences of HO-2mRNA and HO-2 protein among these 4 groups (both P > 0.05). It is concluded that upregulation of the HO-1 protein might contribute to the protection of kidney during septic shock in rats.
Yu
Jian-Bo
JB
Department of Anesthesiology, Tianjin Medical University, Tianjin, China. jianboyu99@yahoo.com.cn
Zhou
Fen
F
Yao
Shang-Long
SL
Tang
Zhao-Hui
ZH
Wang
Man
M
Chen
Hui-Rong
HR
D000818:Animals; D002248:Carbon Monoxide / Q000097:blood; D015971:Gene Expression Regulation, Enzymologic / Q000502:physiology; D006419:Heme Oxygenase (Decyclizing) / Q000235:genetics / Q000378:metabolism; D051547:Heme Oxygenase-1 / Q000235:genetics* / Q000378:metabolism*; D007668:Kidney / Q000201:enzymology*; D007677:Kidney Function Tests; D008070:Lipopolysaccharides / Q000494:pharmacology; D008297:Male; D008315:Malondialdehyde / Q000097:blood; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D012772:Shock, Septic / Q000139:chemically induced / Q000378:metabolism* / Q000503:physiopathology*; D013482:Superoxide Dismutase / Q000097:blood; D015854:Up-Regulation / Q000502:physiology
D008070:Lipopolysaccharides; D012333:RNA, Messenger; D008315:Malondialdehyde; D002248:Carbon Monoxide; D006419:Heme Oxygenase (Decyclizing); D051547:Heme Oxygenase-1; C105245:heme oxygenase-2; D013482:Superoxide Dismutase