DSA affinity glycoproteome of human liver tissue.
Due to the critical roles of glycoproteins in the activities of cells to tissues, mapping of liver glycoproteome may provide valuable basic information for finding disease marker proteins. In this study, Datura Stramonium Agglutinin (DSA) was chosen to enrich N-linked glycoproteins for its broader specificity with tri- or tetra-antennary complex type. DSA affinity glycoproteins' profiles of human liver tissue were generated by two-dimensional electrophoresis (2-DE) followed by glycoprotein staining based on multiplexed proteomics (MP) technology. 64+/-3 (n=3) protein spots were detected and 41 of glycoproteins were identified via peptide mass fingerprinting (PMF) using MALDI-TOF-MS/MS and annotated to IPI databases. Identified glycoproteins definitely take part in the regulation of cell cycle and metabolic processes. The detailed carbohydrate moiety of some glycoproteins might be concluded according to the literatures. The construction of DSA affinity glycoprotein database would contribute to the subsequent research.
Sun
Qiangling
Q
Liver Cancer Institute, Zhongshan Hospital, Fudan University, Shanghai 200032, China.
Kang
Xiaonan
X
Zhang
Yang
Y
Zhou
Haijun
H
Dai
Zhi
Z
Lu
Wenjing
W
Zhou
Xinwen
X
Liu
Xiaohui
X
Yang
Pengyuan
P
Liu
Yinkun
Y
D000328:Adult; D002846:Chromatography, Affinity; D030562:Databases, Protein; D015180:Electrophoresis, Gel, Two-Dimensional; D006023:Glycoproteins / Q000378:metabolism*; D006801:Humans; D008099:Liver / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D037121:Plant Lectins / Q000378:metabolism*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
C041384:Datura stramonium lectin; D006023:Glycoproteins; D037121:Plant Lectins