title: Expression of hMLH1 and hMSH2 proteins in pleomorphic adenoma of minor salivary glands: relationship with clinical and histologic findings.
abstract: OBJECTIVE
  The aim was to examine the relationship between clinicopathological features and immunoexpression of hMLH1 and hMSH2 proteins in pleomorphic adenoma (PA) of minor salivary glands.
  
  
  METHODS
  Paraffin-embedded samples of typically benign PA lesions (n = 35) were prepared for histologic and immunohistochemical assessment. Based on the clinicopathologic features, the samples were categorized into low- and high-risk subtypes for their estimated potential for malignant transformation.
  
  
  RESULTS
  Immunohistochemical analysis revealed strong correlations regarding the expression estimation and staining-intensity distribution (SID) scores between the two proteins. Although there was no relationship between marker immunoexpression and SID scores regarding clinical parameters, statistically significant variations for these parameters were evident regarding some histologic criteria or for risk stratification subtypes.
  
  
  CONCLUSIONS
  The findings of this study could suggest the relationship of a DNA mismatch repair deficiency with high-risk subtypes of PA and the implication of its role in the origin and progression of these subtypes.
authors.affiliation: Laboratory of Immunodetection and Bioanalysis, Faculty of Dentistry, University of Antioquia, Medellín, Colombia. labinbio@odontologia.udea.edu.co
authors.forename: Sergio Iván
authors.initials: SI
authors.lastname: Tobón-Arroyave
authors.forename: Gloria Amparo
authors.initials: GA
authors.lastname: Flórez-Moreno
authors.forename: Juan Fernando
authors.initials: JF
authors.lastname: Jaramillo-Cárdenas
authors.forename: Juan David
authors.initials: JD
authors.lastname: Arango-Uribe
authors.forename: Diana María
authors.initials: DM
authors.lastname: Isaza-Guzmán
authors.forename: Javier
authors.initials: J
authors.lastname: Rendón-Henao
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000378:metabolism*; D008949:Adenoma, Pleomorphic / Q000378:metabolism*; D000293:Adolescent; D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000378:metabolism; D002648:Child; D053843:DNA Mismatch Repair / Q000502:physiology; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D000070957:MutL Protein Homolog 1; D051718:MutS Homolog 2 Protein / Q000378:metabolism*; D009687:Nuclear Proteins / Q000378:metabolism*; D012468:Salivary Gland Neoplasms / Q000378:metabolism* / Q000473:pathology; D012470:Salivary Glands, Minor / Q000378:metabolism* / Q000473:pathology
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D014408:Biomarkers, Tumor; C501486:MLH1 protein, human; D009687:Nuclear Proteins; C497172:MSH2 protein, human; D000070957:MutL Protein Homolog 1; D051718:MutS Homolog 2 Protein