Pseudomonas aeruginosa las and rhl quorum-sensing systems are important for infection and inflammation in a rat prostatitis model.
Pseudomonas aeruginosa frequently acts as an opportunistic pathogen of mucosal surfaces; yet, despite causing aggressive prostatitis in some men, its role as a pathogen in the prostate has not been investigated. Consequently, we developed a Ps. aeruginosa infection model in the rat prostate by instilling wild-type (WT) Ps. aeruginosa strain PAO1 into the rat prostate. It was found that Ps. aeruginosa produced acute and chronic infections in this mucosal tissue as determined by bacterial colonization, gross morphology, tissue damage and inflammatory markers. WT strain PAO1 and its isogenic mutant PAO-JP2, in which both the lasI and rhlI quorum-sensing signal systems have been silenced, were compared during both acute and chronic prostate infections. In acute infections, bacterial numbers and inflammatory markers were comparable between WT PA01 and PAO-JP2; however, considerably less tissue damage occurred in infections with PAO-JP2. Chronic infections with PAO-JP2 resulted in reduced bacterial colonization, tissue damage and inflammation as compared to WT PAO1 infections. Therefore, the quorum-sensing lasI and rhlI genes in Ps. aeruginosa affect acute prostate infections, but play a considerably more important role in maintaining chronic infections. We have thus developed a highly reproducible model for the study of Ps. aeruginosa virulence in the prostate.
Nelson
Lisa K
LK
Biofilm Research Group, Department of Biological Sciences, University of Calgary, 2500 University Drive NW, Calgary, AB T2N 1N4, Canada.
D'Amours
Genevieve H
GH
Biofilm Research Group, Department of Biological Sciences, University of Calgary, 2500 University Drive NW, Calgary, AB T2N 1N4, Canada.
Sproule-Willoughby
Kimberley M
KM
Biofilm Research Group, Department of Biological Sciences, University of Calgary, 2500 University Drive NW, Calgary, AB T2N 1N4, Canada.
Morck
Douglas W
DW
Biofilm Research Group, Department of Biological Sciences, University of Calgary, 2500 University Drive NW, Calgary, AB T2N 1N4, Canada.
Ceri
Howard
H
Biofilm Research Group, Department of Biological Sciences, University of Calgary, 2500 University Drive NW, Calgary, AB T2N 1N4, Canada.
D000818:Animals; D001426:Bacterial Proteins / Q000378:metabolism*; D016207:Cytokines / Q000378:metabolism; D007249:Inflammation; D008025:Ligases / Q000378:metabolism*; D008297:Male; D023421:Models, Animal; D011467:Prostate / Q000382:microbiology / Q000473:pathology / Q000503:physiopathology; D011472:Prostatitis / Q000209:etiology* / Q000378:metabolism / Q000503:physiopathology; D011552:Pseudomonas Infections / Q000150:complications / Q000378:metabolism / Q000382:microbiology*; D011550:Pseudomonas aeruginosa / Q000472:pathogenicity / Q000502:physiology; D053038:Quorum Sensing; D051381:Rats; D013997:Time Factors; D014157:Transcription Factors / Q000378:metabolism*; D014774:Virulence
D001426:Bacterial Proteins; D016207:Cytokines; C081237:LasI protein, Pseudomonas aeruginosa; D014157:Transcription Factors; D008025:Ligases; C096955:RHLI protein, Pseudomonas aeruginosa