Validation of a multi-residue liquid chromatography-tandem mass spectrometry confirmatory method for 10 anticoccidials in eggs according to Commission Decision 2002/657/EC.
A liquid chromatography-tandem mass spectrometric (LC-MS/MS) method for the simultaneous detection and confirmation of halofuginone, robenidine, diclazuril, nicarbazin, monensin, narasin, lasalocid, salinomycin, maduramicin and semduramicin in whole egg has been developed and validated. The anticoccidial residues were extracted by acetonitrile, evaporated and dissolved in a sodium acetate/acetonitrile mixture. Then, the samples were injected on a C8 column in a gradient mode. Diclazuril-bis, DNC-d8 and nigericin were used as internal standards. The results of the full validation in accordance with the guidelines of the Commission Decision no 2002/657/EC are presented. This rapid and sensitive method was found suitable to confirm the anticoccidials at 1 and at 75 microg kg(-1) for the MRL compound lasalocid.
Dubreil-Chéneau
Estelle
E
Veterinary Drug Residues Unit, Laboratory for the Research and Investigation of Veterinary Drugs and Disinfectants, AFSSA Fougères, BP 90203 La Haute Marche, 35133 Javené, France. e.dubreil@fougeres.afssa.fr
Bessiral
Mélaine
M
Roudaut
Brigitte
B
Verdon
Eric
E
Sanders
Pascal
P
D000818:Animals; D002645:Chickens; D002853:Chromatography, Liquid / Q000379:methods*; D003049:Coccidiostats / Q000737:chemistry*; D004350:Drug Residues / Q000737:chemistry*; D004531:Eggs / Q000032:analysis*; D053719:Tandem Mass Spectrometry / Q000379:methods*
D003049:Coccidiostats