Blood lipid-lowering and antioxidant effects of a structured lipid containing monoacylglyceride enriched with monounsaturated fatty acids in C57BL/6 mice.
We recently reported that a synthetic edible oil-containing monoacylglyceride (MAG) and diacylglyceride (DAG) exerted anti-atherosclerotic effects. In order to further investigate the activities and individual effects of MAG and DAG on the atherosclerotic process, we prepared a structured oil with various MAG and DAG contents and tested them both in vitro and in vivo, using C57BL/6 mice. The structured oil to be tested was mixed (final concentration 5%, wt/wt) with a high-cholesterol high-fat diet (1.2% cholesterol/15% fat/0.5% sodium cholate) and provided to the mice for 7 weeks. After administration, the mice consuming MAG97%-oil and DAG50%/MAG10%-oil evidenced 17% and 24% decreases in plasma total cholesterol (TC) level, respectively, as compared to a group of mice fed on lard. The experimental mice also had reduced plasma triglyceride concentrations and elevated high-density lipoprotein-cholesterol to TC ratios, by up to 31% in the case of the DAG50%/MAG10%-oil fed mice. The mice fed on MAG97%-oil exhibited elevated plasma antioxidant activity and lecithin:cholesterol acyltransferase activity. Histological assessments of the livers of the mice showed that the consumption of MAG-containing oil attenuated the adhesion of inflammatory cells and also ameliorated fatty liver changes, as compared to what was observed in the case of DAG85%-oil consumption. In conclusion, the MAG-containing oil exhibited anti-inflammatory and antioxidant activities in vivo, as well as in vitro inhibitory activity against human cholesteryl ester transfer protein. These results provide us with new insights into MAG-containing oil in terms of hypocholesterolemic effects and antioxidant activities.
Cho
Kyung-Hyun
KH
School of Biotechnology, Yeungnam University, Gyeongsan, Republic of Korea.
Lee
Jeung-Hee
JH
Kim
Jin-Man
JM
Park
Sang Hyun
SH
Choi
Myung-Sook
MS
Lee
Yun-Mi
YM
Choi
Inho
I
Lee
Ki-Teak
KT
D000217:Acyltransferases / Q000097:blood; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D000924:Anticholesteremic Agents / Q000494:pharmacology*; D000975:Antioxidants / Q000378:metabolism / Q000494:pharmacology*; D001053:Apolipoproteins / Q000097:blood; D050197:Atherosclerosis / Q000517:prevention & control; D002784:Cholesterol / Q000097:blood; D053480:Cholesterol Ester Transfer Proteins / Q000037:antagonists & inhibitors; D004032:Diet; D004041:Dietary Fats / Q000494:pharmacology; D004075:Diglycerides / Q000494:pharmacology*; D005229:Fatty Acids, Monounsaturated / Q000494:pharmacology*; D005234:Fatty Liver / Q000378:metabolism / Q000473:pathology; D000960:Hypolipidemic Agents / Q000494:pharmacology*; D008055:Lipids; D008099:Liver / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D050178:Monoglycerides / Q000494:pharmacology*; D000069463:Olive Oil; D010938:Plant Oils / Q000378:metabolism; D014280:Triglycerides / Q000097:blood
D000893:Anti-Inflammatory Agents; D000924:Anticholesteremic Agents; D000975:Antioxidants; D001053:Apolipoproteins; D053480:Cholesterol Ester Transfer Proteins; D004041:Dietary Fats; D004075:Diglycerides; D005229:Fatty Acids, Monounsaturated; D000960:Hypolipidemic Agents; D008055:Lipids; D050178:Monoglycerides; D000069463:Olive Oil; D010938:Plant Oils; D014280:Triglycerides; D002784:Cholesterol; D000217:Acyltransferases; C029310:lard