Effect of compound plate composition on measurement of hERG current IC(50) using PatchXpress.
Inhibition of the human ether-a-go-go-related gene (hERG) potassium channel by pharmaceutical agents can lead to acquired long QT syndrome and the generation of potentially lethal arrhythmias. Higher throughput automated patch clamp systems, such as PatchXpress, can greatly increase the speed and capacity of evaluation of pharmaceutical compounds for hERG blocking activity. A factor that may affect the IC(50) value of a compound measured in this system is the composition of the multi-well compound plate. Hydrophobic compounds may adsorb to the surfaces of multi-well plates resulting in a reduction in the effective concentration of the compound delivered to the cell and altered IC(50) values. In the present study, we investigated the effects of four different compound plates--glass vials, non-binding polystyrene, hydrophilic polystyrene, and polystyrene--on determination of IC(50)s for four compounds--sotalol, dofetilide, cisapride, and bepridil--which ranged in hydrophobicity. In addition, we investigated the effects of incubation time in the compound plate on determination of IC(50)s. hERG currents were measured using the PatchXpress 7000A Automated Parallel Patch Clamp System (Molecular Devices Corporation; Sunnyvale, CA) and hERG channels stably expressed in HEK293 cells. The results suggest that more hydrophobic compounds may adsorb to non-binding polystyrene, hydrophilic, and polystyrene compound plates versus glass plates, especially with increasing time on the plates, resulting in altered IC(50) values.
Mo
Zun-Li
ZL
Pfizer Global Research and Development, Drug Safety Research and Development, Chesterfield, MO 63017, USA. Zun-li.Mo@Pfizer.com
Faxel
Tracy
T
Yang
Young-Sun
YS
Gallavan
Robert
R
Messing
Dean
D
Bahinski
Anthony
A
D000327:Adsorption; D015764:Bepridil / Q000494:pharmacology; D002460:Cell Line; D020117:Cisapride / Q000494:pharmacology; D051638:Ether-A-Go-Go Potassium Channels / Q000037:antagonists & inhibitors* / Q000502:physiology*; D005898:Glass; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D018408:Patch-Clamp Techniques / Q000295:instrumentation / Q000379:methods*; D010627:Phenethylamines / Q000494:pharmacology; D011137:Polystyrenes; D015203:Reproducibility of Results; D013015:Sotalol / Q000494:pharmacology; D013449:Sulfonamides / Q000494:pharmacology
D051638:Ether-A-Go-Go Potassium Channels; D010627:Phenethylamines; D011137:Polystyrenes; D013449:Sulfonamides; D015764:Bepridil; D013015:Sotalol; C063533:dofetilide; D020117:Cisapride