Sonochemically prepared BSA microspheres containing Gemcitabine, and their potential application in renal cancer therapeutics.
This report demonstrates the formation and characterization of sonochemically prepared bovine serum albumin (BSA)-Gemzar (Gemcitabine) microspheres and shows their increased anticancer activity compared to pristine Gemzar. The amount of loaded Gemzar was determined by light absorption measurements. The BSA-Gemzar composite was analyzed and characterized by optical microscopy and scanning electron microscopy. The release kinetics of Gemzar from the proteinaceous microspheres was tested. The BSA-Gemzar composite was examined for its anticancer activity (in vitro) in renal cancer cells (RCC, 786-O cells) using [(3)H]thymidine incorporation assays. It was found that the influence of the Gemzar-loaded microspheres on the cancer cells was significantly greater than that of an equimolar concentration of pristine Gemzar.
Grinberg
Olga
O
Department of Chemistry, Kanbar Laboratory for Nanomaterials, Nanotechnology Research Center, Institute of Nanotechnology and Advanced Materials, Bar-Ilan University, Ramat-Gan 52900, Israel.
Gedanken
Aharon
A
Patra
Chitta Ranjan
CR
Patra
Sujata
S
Mukherjee
Priyabrata
P
Mukhopadhyay
Debabrata
D
D000818:Animals; D000964:Antimetabolites, Antineoplastic / Q000008:administration & dosage / Q000737:chemistry; D017209:Apoptosis / Q000187:drug effects; D002292:Carcinoma, Renal Cell / Q000188:drug therapy / Q000503:physiopathology*; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D003841:Deoxycytidine / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000737:chemistry; D004058:Diffusion; D004337:Drug Carriers / Q000737:chemistry* / Q000528:radiation effects; D004339:Drug Compounding / Q000379:methods; D005240:Feasibility Studies; D008863:Microspheres; D051381:Rats; D012710:Serum Albumin, Bovine / Q000737:chemistry* / Q000528:radiation effects / Q000648:ultrastructure; D013010:Sonication / Q000379:methods*; D000093542:Gemcitabine
D000964:Antimetabolites, Antineoplastic; D004337:Drug Carriers; D003841:Deoxycytidine; D012710:Serum Albumin, Bovine; D000093542:Gemcitabine