DNA characterization of simian Entamoeba histolytica-like strains to differentiate them from Entamoeba histolytica.
Two simian Entamoeba histolytica-like strains, EHMfas1 and P19-061405, have been suggested to represent a new species based on genetic characterization. Sequence analyses of the hexokinase, glucose phosphate isomerase, and phosphoglucomutase genes supported the previous findings of isoenzyme analyses demonstrating a new zymodeme pattern. Phylogenetic studies of 18S rDNA, 5.8S rDNA, the chaperonin 60 gene, and the pyridine nucleotide transhydrogenase gene showed original clusters of simian E. histolytica-like strains below or near E. histolytica, respectively. Comparative studies of the chitinase and the serine-rich E. histolytica protein genes and locus 1-2 region revealed that most mutated units were shared among the simian E. histolytica-like strains. The similarities of each of the repeating units within the simian E. histolytica-like strains or E. histolytica and the differences of those between the both might be generated by concerted evolution. Our results indicate that EHMfas1 and P19-061405 should be considered to be the same species, despite that they were isolated from different monkey species and different habitats. Simian E. histolytica-like amebas may be endemic to macaque monkeys, as a counterpart to E. histolytica in humans, and should be differentiated from E. histolytica by the revival name Entamoeba nuttalli, as proposed for P19-061405.
Takano
Jun-ichiro
J
The Corporation for Production and Research of Laboratory Primates, 1-1 Hachimandai, Tsukuba, Ibaraki 305-0843, Japan. takano@primate.or.jp
Tachibana
Hiroshi
H
Kato
Miyoko
M
Narita
Toyoko
T
Yanagi
Tetsuo
T
Yasutomi
Yasuhiro
Y
Fujimoto
Koji
K
D000818:Animals; D016000:Cluster Analysis; D016054:DNA, Protozoan / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D004747:Entamoeba / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D004749:Entamoebiasis / Q000662:veterinary*; D005838:Genotype; D005956:Glucose-6-Phosphate Isomerase / Q000235:genetics; D000882:Haplorhini / Q000469:parasitology*; D006593:Hexokinase / Q000235:genetics; D008969:Molecular Sequence Data; D008992:Monkey Diseases / Q000469:parasitology*; D010733:Phosphoglucomutase / Q000235:genetics; D010802:Phylogeny; D012337:RNA, Ribosomal, 18S / Q000235:genetics; D012340:RNA, Ribosomal, 5.8S / Q000235:genetics; D017422:Sequence Analysis, DNA
D016054:DNA, Protozoan; D004275:DNA, Ribosomal; D012337:RNA, Ribosomal, 18S; D012340:RNA, Ribosomal, 5.8S; D006593:Hexokinase; D005956:Glucose-6-Phosphate Isomerase; D010733:Phosphoglucomutase