title: Proteomic identification and profiling of canine lymphoma patients.
abstract: This study employed proteomic and bioinformatic approaches to identify serum biomarkers in canine lymphoma patients. Chilled serum samples derived from non-lymphoma (n = 92) and lymphoma (n = 87) patients were shipped from first opinion veterinary practices, subjected to ion exchange chromatography and analysed by surface-enhanced laser desorption ionization mass spectrometry. Nineteen serum protein peaks were identified between the two groups as being significantly different (P < 0.05) based upon their normalized ion intensities. Two biomarkers were identified that were capable of differentiating lymphoma and non-lymphoma patients. Analysis of the test data provided a positive predictive value (PPV) of 82%. A clinical follow-up study was carried out on 96 canine patients suspected of having lymphoma. Evaluation of this data gave a specificity value of 91%, sensitivity of 75%, PPV of 80% and negative predictive value of 88%. In conclusion, the expression pattern of two serum biomarkers has enabled serum samples to be classified into either lymphoma or non-lymphoma categories.
authors.affiliation: PetScreen Ltd, BioCity, Nottingham, UK.
authors.forename: L
authors.initials: L
authors.lastname: Ratcliffe
authors.forename: S
authors.initials: S
authors.lastname: Mian
authors.forename: K
authors.initials: K
authors.lastname: Slater
authors.forename: H
authors.initials: H
authors.lastname: King
authors.forename: M
authors.initials: M
authors.lastname: Napolitano
authors.forename: D
authors.initials: D
authors.lastname: Aucoin
authors.forename: A
authors.initials: A
authors.lastname: Mobasheri
mesh_terms: D000367:Age Factors; D000818:Animals; D014408:Biomarkers, Tumor; D001798:Blood Proteins / Q000032:analysis*; D016022:Case-Control Studies; D003937:Diagnosis, Differential; D004283:Dog Diseases / Q000097:blood*; D004285:Dogs; D008223:Lymphoma / Q000097:blood / Q000662:veterinary*; D011237:Predictive Value of Tests; D040901:Proteomics; D012680:Sensitivity and Specificity; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000662:veterinary*
chemical_list: D014408:Biomarkers, Tumor; D001798:Blood Proteins