Pre-staining paper chromatography method for quantification of gamma-aminobutyric acid.
The routine method of paper chromatography includes five steps: spotting, separating, drying, spraying/immersing and color development. In this paper, a pre-staining paper chromatography which only consisted of spotting, separating and color development was developed for quantitative analysis of gamma-aminobutyric acid. Compared to the routine paper chromatography, the improved method is clean, rapid, inexpensive and reproducible. The effects of ninhydrin concentration, color temperature, color time and Cu(2+) concentration on the color yield in the ninhydrin reaction were optimized. And then the pre-staining paper chromatography coupled with vis spectrophotometry was applied to gamma-aminobutyric acid quantification. The results indicated that the limit of detection was 0.05 mg mL(-1) and the linear range was from 0.5 to 20.0 mg mL(-1). Furthermore, an excellent correlation coefficient was observed with an R(2)=0.998. The method is accurate (RSD<2.64%), and has good recoveries (102.7-103.9%). The validation of the modified technique was verified by a HPLC method.
Li
Haixing
H
Sino-German Joint Research Institute, Nanchang University, Nanchang, China.
Qiu
Ting
T
Cao
Yusheng
Y
Yang
Jiyan
J
Huang
Zhibing
Z
D002851:Chromatography, High Pressure Liquid; D002854:Chromatography, Paper / Q000379:methods*; D003300:Copper / Q000737:chemistry; D007202:Indicators and Reagents / Q000737:chemistry; D016014:Linear Models; D009555:Ninhydrin / Q000737:chemistry; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013696:Temperature; D005680:gamma-Aminobutyric Acid / Q000032:analysis*
D007202:Indicators and Reagents; D005680:gamma-Aminobutyric Acid; D003300:Copper; D009555:Ninhydrin