title: [Effects of 18beta-glycyrrhizic acid and 18alpha-glycyrrhizic acid on mRNA and protein expression of cytochrome P450 3A in cultured rat primary hepatocyte].
abstract: OBJECTIVE
  To study the effects of 18beta-glycyrrhizic acid and 18alpha-glycyrrhizic acid on mRNA and protein expression of cytochrome P450 3A in cultured rat primary hepatocyte.
  
  
  METHODS
  Rat heaptocyte were isolated by two-step in situ collagenase perfusion method; the hepatocytes were seeded in dishes coated with type I rat tail collagen, culture medium was added and changed daily after gelation, the effect of 18beta-glycyrrhizic acid and 18alpha-glycyrrhizic acid on the expression of CYP3A was determined by reverse transcriptase-polymerase chain reaction (RT-PCR) and western blot respectively.
  
  
  RESULTS
  Treatment with 18beta-glycyrrhizic acid of primary rat hepatocytes resulted in marked up-regulation of CYP3A1 expression at both mRNA and protein levels in a concentration dependent manner, while exposure to 18alpha-glycyrrhizic acid of primary rat hepatocytes resulted in marked down-regulation of CYP3A1 expression at both mRNA and protein levels in a concentration dependent manmer.
  
  
  CONCLUSIONS
  18beta-glycyrrhizic acid and 18alpha-glycyrrhizic acid up-regulate and down-regulate CYP3A1 expression at the transcriptive levels.
authors.affiliation: Beijing Institute of Radiation Medicine, Beijing 100850, China.
authors.forename: Yuguang
authors.initials: Y
authors.lastname: Wang
authors.forename: Minghui
authors.initials: M
authors.lastname: Yang
authors.forename: Zengchun
authors.initials: Z
authors.lastname: Ma
authors.forename: Qiande
authors.initials: Q
authors.lastname: Liang
authors.forename: Hongling
authors.initials: H
authors.lastname: Tan
authors.forename: Chengrong
authors.initials: C
authors.lastname: Xiao
authors.forename: Yue
authors.initials: Y
authors.lastname: Gao
mesh_terms: D000818:Animals; D019411:Clinical Laboratory Techniques; D051544:Cytochrome P-450 CYP3A / Q000235:genetics / Q000378:metabolism*; D019695:Glycyrrhizic Acid / Q000494:pharmacology*; D022781:Hepatocytes / Q000187:drug effects* / Q000378:metabolism; D014176:Protein Biosynthesis / Q000187:drug effects*; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D017208:Rats, Wistar; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D014158:Transcription, Genetic / Q000187:drug effects* / Q000235:genetics
chemical_list: D012333:RNA, Messenger; D019695:Glycyrrhizic Acid; D051544:Cytochrome P-450 CYP3A