Differential protein expression in the corpus callosum (body) of human alcoholic brain.
Neuroimage analysis in alcoholic corpus callosum (CC) suggests that microstructural abnormalities are higher in the genu followed by the body and the splenium. Molecular mechanisms underlying these dysmorphologys are still unclear. Protein expression was performed using the CC body samples [(nine controls, seven uncomplicated, and six complicated (with liver cirrhosis) alcoholics] through proteomics approach. Thirty-nine protein spots in uncomplicated and 60 in complicated alcoholics were differentially altered compared with the control (p < 0.05). Comparison between alcoholic groups revealed that 40% more protein showed altered expression in complicated compared with uncomplicated. This result suggests that alcohol-related liver dysfunction has synergetic effects on brain protein expression. Subregional expression profiles indicate that the highest numbers of region-specific proteins were in the genus followed by the CC body and the splenium. Interestingly, abnormal thiamine cascade was strongly suggested in the genu, and to a lesser extent in the CC body, but no such cascade was observed in the splenium. Therefore, alcohol-induced microstructural damage detected by image analysis in the CC, possibly involves multiple biochemical mechanisms.
Kashem
Mohammed Abul
MA
University of Sydney, New South Wales, Australia. kabul@psych.usyd.edu.au
Etages
Huyghues Des
HD
Kopitar-Jerala
Natasa
N
McGregor
Iain S
IS
Matsumoto
Izuru
I
D000437:Alcoholism / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D003337:Corpus Callosum / Q000473:pathology / Q000502:physiology*; D020869:Gene Expression Profiling / Q000379:methods*; D005786:Gene Expression Regulation / Q000502:physiology*; D005904:Glial Fibrillary Acidic Protein / Q000096:biosynthesis / Q000235:genetics; D006801:Humans; D018384:Oxidative Stress / Q000502:physiology; D014176:Protein Biosynthesis / Q000235:genetics; D040901:Proteomics / Q000379:methods; D012177:Retinol-Binding Proteins / Q000096:biosynthesis / Q000235:genetics; D015398:Signal Transduction / Q000502:physiology
D005904:Glial Fibrillary Acidic Protein; D012177:Retinol-Binding Proteins