title: Characterization of crustin antimicrobial proteins from Japanese spiny lobster Panulirus japonicus.
abstract: Crustin antimicrobial proteins (PJC1-4) were identified from a phyllosoma library of Japanese spiny lobster, Panulirus japonicus. The deduced amino acid sequences of PJC1-4 contained open reading frames of 130, 139, 124 and 150 amino acid residues, respectively. These proteins contained a glycine-rich region at the N-terminus and 12 conserved cysteine residues containing a single whey acidic protein (WAP) domain at the C-terminus. A phylogenetic tree and sequences alignment analyses revealed that PJC1-4 are more closely related to shrimp crustins than to other lobster crustins. Transcripts of PJC1, 3 and 4 were detected in heart, nerves, intestine, hemocytes, gills and hepatopancreas, while transcripts of PJC2 were detected only in nerves.
authors.affiliation: Laboratory of Genome Science, Tokyo University of Marine Science and Technology, Konan 4-5-7, Minato-ku, Tokyo, 108-8477, Japan.
authors.forename: Duangjai
authors.initials: D
authors.lastname: Pisuttharachai
authors.forename: Fernand F
authors.initials: FF
authors.lastname: Fagutao
authors.forename: Motoshige
authors.initials: M
authors.lastname: Yasuike
authors.forename: Hideaki
authors.initials: H
authors.lastname: Aono
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Yano
authors.forename: Keisuke
authors.initials: K
authors.lastname: Murakami
authors.forename: Hidehiro
authors.initials: H
authors.lastname: Kondo
authors.forename: Takashi
authors.initials: T
authors.lastname: Aoki
authors.forename: Ikuo
authors.initials: I
authors.lastname: Hirono
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D023181:Antimicrobial Cationic Peptides / Q000145:classification / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D020869:Gene Expression Profiling; D008969:Molecular Sequence Data; D033521:Palinuridae / Q000235:genetics*; D010802:Phylogeny; D020033:Protein Isoforms / Q000145:classification / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D014158:Transcription, Genetic
chemical_list: D023181:Antimicrobial Cationic Peptides; D018076:DNA, Complementary; D020033:Protein Isoforms; D012333:RNA, Messenger