title: Influence of antidepressant drugs on Ecto-nucleotide pyrophosphatase/phosphodiesterases (E-NPPs) from salivary glands of rats.
abstract: Xerostomia is commonly caused by antidepressant drugs and ATP can influence the saliva production. Adenosine is the product of extracellular hydrolysis of adenine nucleotides in submandibular gland cells, which occurs by the action of ectonucleotidases. In this study, we have evaluated the effect of three different antidepressants in ecto-nucleotide pyrophosphatase/phosphodiesterase (E-NPP1-3) activities in cultured cells from salivary glands. Rats received imipramine (10mg/ml), fluoxetine (20mg/ml) or moclobemide (30mg/ml) by oral gavage. The drugs were administered once a day for 14 days. Our results have shown that the hydrolysis of p-nitrophenyl-5'-thymidine monophosphate increased in all treatments. These effects were not consequence of transcriptional control of E-NPP1-3 genes. The results reported here can highlight the importance of ectonucleotidases in the most common side effect caused by antidepressant therapy.
authors.affiliation: Departamento de Bioquímica, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, RS, Brazil.
authors.forename: Sandra Liana
authors.initials: SL
authors.lastname: Henz
authors.forename: Giana de Paula
authors.initials: Gde P
authors.lastname: Cognato
authors.forename: Fernanda Cenci
authors.initials: FC
authors.lastname: Vuaden
authors.forename: Maurício Reis
authors.initials: MR
authors.lastname: Bogo
authors.forename: Carla Denise
authors.initials: CD
authors.lastname: Bonan
authors.forename: João José Freitas
authors.initials: JJ
authors.lastname: Sarkis
mesh_terms: D000818:Animals; D000928:Antidepressive Agents / Q000494:pharmacology*; D018687:Antidepressive Agents, Second-Generation / Q000494:pharmacology; D000929:Antidepressive Agents, Tricyclic / Q000494:pharmacology; D002478:Cells, Cultured; D005473:Fluoxetine / Q000494:pharmacology; D006868:Hydrolysis; D007099:Imipramine / Q000494:pharmacology; D008297:Male; D020912:Moclobemide / Q000494:pharmacology; D010727:Phosphoric Diester Hydrolases / Q000032:analysis / Q000187:drug effects*; D010766:Phosphorylation; D011755:Pyrophosphatases / Q000032:analysis / Q000187:drug effects*; D051381:Rats; D017208:Rats, Wistar; D012471:Salivary Proteins and Peptides / Q000032:analysis / Q000187:drug effects; D013363:Submandibular Gland / Q000166:cytology / Q000187:drug effects / Q000201:enzymology*; D013938:Thymidine Monophosphate / Q000031:analogs & derivatives / Q000032:analysis; D013997:Time Factors; D000099189:Lysophospholipase D
chemical_list: D000928:Antidepressive Agents; D018687:Antidepressive Agents, Second-Generation; D000929:Antidepressive Agents, Tricyclic; D005473:Fluoxetine; D007099:Imipramine; D020912:Moclobemide; D010727:Phosphoric Diester Hydrolases; D011755:Pyrophosphatases; D012471:Salivary Proteins and Peptides; D013938:Thymidine Monophosphate; D000099189:Lysophospholipase D; C032579:thymidine 5'-4-nitrophenyl phosphate; C524442:Enpp3 protein, rat; C065962:ectonucleotide pyrophosphatase phosphodiesterase 1