Comparison of the activity of human and bovine milk on two cell lines.
The activity of human milk on cell growth has been evaluated on two cell lines, MDCK and Caco-2. The proportion of human milk samples that reduced by half the growth of MDCK cells was of 36%. This inhibitory activity was associated with casein and not the whey fraction. Great variability was found in the degree of inhibitory activity depending on the milk sample. The susceptibility of Caco-2 cells to milk inhibitory activity was lower than that of MDCK. Bovine milk did not have any effect on cell growth, either as skimmed milk or as whey or casein. Morphology of cells incubated with active human casein showed abnormal features, such as chromatin condensation, reduced cellular volume and apoptotic bodies, and also fragmented DNA, which are all features of apoptosis.
Pocoví
Coloma
C
Tecnología de los Alimentos, Miguel Servet, 177, Zaragoza 50013, Spain.
Conesa
Celia
C
Barbana
Chockry
C
Pérez
María D
MD
Calvo
Miguel
M
Sánchez
Lourdes
L
D000818:Animals; D018938:Caco-2 Cells; D002364:Caseins / Q000008:administration & dosage* / Q000302:isolation & purification; D002417:Cattle; D002455:Cell Division / Q000187:drug effects*; D002460:Cell Line; D003470:Culture Media; D053938:DNA Fragmentation / Q000187:drug effects; D004285:Dogs; D005223:Fats / Q000032:analysis; D006358:Hot Temperature; D006801:Humans; D007668:Kidney; D007768:Lactalbumin / Q000032:analysis; D007781:Lactoferrin / Q000032:analysis; D008892:Milk / Q000737:chemistry*; D008894:Milk Proteins / Q000008:administration & dosage; D008895:Milk, Human / Q000737:chemistry*; D000067816:Whey Proteins
D002364:Caseins; D003470:Culture Media; D005223:Fats; D008894:Milk Proteins; D000067816:Whey Proteins; D007768:Lactalbumin; D007781:Lactoferrin