Differential changes in GPR55 during microglial cell activation.
We examined how lipopolysaccharide (LPS) and interferon gamma (IFN-gamma), known to differentially activate microglia, affect the expression of G protein-coupled receptor 55 (GPR55), a novel cannabinoid receptor. We found that GPR55 mRNA is significantly expressed in both primary mouse microglia and the BV-2 mouse microglial cell line, and that LPS down-regulates this message. Conversely, IFN-gamma slightly decreases GPR55 mRNA in primary microglia, while it upregulates this message in BV-2 cells. Moreover, the GPR55 agonist, lysophosphatidylinositol, increases ERK phosphorylation in BV-2 stimulated with IFN-gamma, in correlation with the increased amount of GPR55 mRNA. Remarkably, these stimuli-induced changes in GPR55 expression are similar to those observed with CB(2)-R, suggesting that both receptors might be involved in neuroinflammation and that their expression is concomitantly controlled by the state of microglial activation.
Pietr
Maciej
M
Neurobiology Department, Weizmann Institute of Science, Rehovot 76100, Israel.
Kozela
Ewa
E
Levy
Rivka
R
Rimmerman
Neta
N
Lin
Yi Hsing
YH
Stella
Nephi
N
Vogel
Zvi
Z
Juknat
Ana
A
D000818:Animals; D001483:Base Sequence; D063386:Cannabinoid Receptor Agonists; D002460:Cell Line; D002478:Cells, Cultured; D017931:DNA Primers / Q000235:genetics; D015870:Gene Expression / Q000187:drug effects; D007371:Interferon-gamma / Q000494:pharmacology; D008070:Lipopolysaccharides / Q000494:pharmacology; D008246:Lysophospholipids / Q000494:pharmacology; D051379:Mice; D017628:Microglia / Q000187:drug effects / Q000378:metabolism*; D019950:Mitogen-Activated Protein Kinase 1 / Q000378:metabolism; D048052:Mitogen-Activated Protein Kinase 3 / Q000378:metabolism; D010766:Phosphorylation; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D043882:Receptors, Cannabinoid / Q000235:genetics* / Q000378:metabolism*; D011994:Recombinant Proteins
D063386:Cannabinoid Receptor Agonists; D017931:DNA Primers; C526692:GPR55 protein, mouse; D008070:Lipopolysaccharides; D008246:Lysophospholipids; D012333:RNA, Messenger; D043882:Receptors, Cannabinoid; D011994:Recombinant Proteins; C025449:lysophosphatidylinositol; D007371:Interferon-gamma; D019950:Mitogen-Activated Protein Kinase 1; D048052:Mitogen-Activated Protein Kinase 3