title: [Genotyping of AKAP10 gene 2073A/G single nucleotide polymorphism by TaqMan probe real-time PCR].
abstract: OBJECTIVE
  To detect AKAP10 gene 2073A/G single nucleotide polymorphism (SNP) genotyping by TaqMan probe real-time PCR.
  
  
  METHODS
  The genotype of AKAP10 gene 2073A/G was detected by real-time PCR with a pair of new-designed TaqMan probes. The PCR products also were subjected to gene sequence analysis to validate the results of TaqMan probe real-time PCR.
  
  
  RESULTS
  The TaqMan probe real-time PCR method was successfully developed to detect AKAP10 gene 2073A/G SNP. The results were accordant with those achieved by DNA sequencing. The distribution of AKAP10 gene 2073A/G among population had no relationship with gender, age (P > 0.05). Unconditional logistic regression analysis revealed that the variant genotypes (AG + GG) had a 52% increased risk of colorectal cancer, compared with the AA genotype (P = 0.019).
  
  
  CONCLUSIONS
  A detection platform for SNP genotyping by TaqMan probe was set up successfully. There was a significant association between AKAP10 gene 2073A/G and colorectal cancer.
authors.affiliation: Institute of Digestive Surgery, West China Hospital, Sichuan University, Chengdu 610041, China.
authors.forename: Mo-jin
authors.initials: MJ
authors.lastname: Wang
authors.forename: Zong-guang
authors.initials: ZG
authors.lastname: Zhou
authors.forename: Ling
authors.initials: L
authors.lastname: Wang
authors.forename: Yuan
authors.initials: Y
authors.lastname: Li
authors.forename: Peng
authors.initials: P
authors.lastname: Zhang
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhang
authors.forename: Chang-fu
authors.initials: CF
authors.lastname: Cui
authors.forename: Bin
authors.initials: B
authors.lastname: Zhou
mesh_terms: D054758:A Kinase Anchor Proteins / Q000235:genetics*; D015179:Colorectal Neoplasms / Q000209:etiology / Q000235:genetics*; D015342:DNA Probes / Q000235:genetics; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods; D020641:Polymorphism, Single Nucleotide; D017422:Sequence Analysis, DNA
chemical_list: D054758:A Kinase Anchor Proteins; C484639:AKAP10 protein, human; D015342:DNA Probes