title: Laetispicine, an amide alkaloid from Piper laetispicum, presents antidepressant and antinociceptive effects in mice.
abstract: In the present work, we studied the effect of laetispicine, an amide alkaloid isolated from the stems of Piper laetispicum (Piperaceae), in forced swimming, open field, acetic acid writhing and formalin tests in KM mice to assess antidepressant and antinociceptive effects. A significant and dose-dependent decrease in the immobility time, as evaluated by the forced swimming test, was observed after laetispicine administration (38.18, 39.79, 58.77 and 67.28% decreased at the doses of 5, 10, 20, 40mg/kg, respectively), suggesting an antidepressant effect. Furthermore, in the open field test, laetispicine at the given doses did not alter the number of crossings and rearing, as compared to controls. Results from writhing and formalin tests showed that laetispicine reduced the number of writhing in mice in a dose-dependent manner, attenuated the licking and spiting time of the injected paw in the first phase of formalin test. The antinociceptive effect of laetispicine was not affected by pre-treatment (i.p.) with naloxone (2mg/kg). In conclusion, we showed that laetispicine possessed significant antidepressant and antinociceptive properties, making this drug potentially useful in depression and pain.
authors.affiliation: School of Pharmacy, Fudan University, Shanghai, China. claireyaocy@hotmail.com
authors.forename: C Y
authors.initials: CY
authors.lastname: Yao
authors.forename: J
authors.initials: J
authors.lastname: Wang
authors.forename: D
authors.initials: D
authors.lastname: Dong
authors.forename: F G
authors.initials: FG
authors.lastname: Qian
authors.forename: J
authors.initials: J
authors.lastname: Xie
authors.forename: S L
authors.initials: SL
authors.lastname: Pan
mesh_terms: D019342:Acetic Acid; D000700:Analgesics / Q000494:pharmacology / Q000627:therapeutic use*; D000818:Animals; D000928:Antidepressive Agents / Q000494:pharmacology / Q000627:therapeutic use*; D001522:Behavior, Animal / Q000187:drug effects*; D052117:Benzodioxoles / Q000494:pharmacology / Q000627:therapeutic use*; D003863:Depression / Q000188:drug therapy; D004305:Dose-Response Relationship, Drug; D005260:Female; D008297:Male; D051379:Mice; D009270:Naloxone / Q000494:pharmacology; D010146:Pain / Q000188:drug therapy; D010147:Pain Measurement / Q000187:drug effects; D008517:Phytotherapy; D031701:Piper / Q000737:chemistry*; D010936:Plant Extracts / Q000494:pharmacology / Q000627:therapeutic use*; D013550:Swimming
chemical_list: D000700:Analgesics; D000928:Antidepressive Agents; D052117:Benzodioxoles; C512035:N-isobutyl-(3,4-methylenedioxyphenyl)-2,4,9-undecatrienoamide; D010936:Plant Extracts; D009270:Naloxone; D019342:Acetic Acid