Biocompatibility of acetazolamide pastes in the subcutaneous tissue of rats.
This aim of this study was to investigate the biocompatibility of two experimental acetazolamide (AZ)-based pastes in the subcutaneous tissue of rats. Both pastes contained AZ as the main component in similar concentration. The vehicle in experimental paste 1 was saline, while experimental paste 2 was prepared with propylene glycol. Sixty polyethylene tubes were sealed at one end with gutta-percha (GP), which served as a control. Half of the tubes were filled with paste 1 and half with paste 2. The tubes were implanted in the subcutaneous tissue of 15 rats, being 4 tubes for each animal. The animals were killed 7, 15 and 45 days after surgery and the specimens were processed in laboratory. The histological sections were stained with hematoxylin and eosin and were analyzed by light microscopy. Scores were assigned to level of inflammatory process: 1- none; 2- mild; 3- moderate; 4- severe. The data were analyzed statistically by the Kruskal-Wallis test (p< or =0.05). Paste 1 produced an inflammatory process at 7 days. However, the intensity of this inflammation decreased with time and was nearly absent at 45 days. No statistically significant difference (p>0.05) was observed between the control (GP) and paste 1. However, paste 2 produced inflammatory response at all study periods and differed significantly (p<0.05) from the control. In conclusion, in the present study, the experimental AZ-based paste 1 was considered as biocompatible as the control matrial (GP), while experimental paste 2 was irritating to rat subcutaneous tissue.
Mori
Graziela Garrido
GG
Department of Integrated Clinic, Adamantina Dental School, Adamantina, SP, Brazil. grazielagm@hotmail.com
Moraes
Ivaldo Gomes de
IG
Nunes
Daniele Clapes
DC
Castilho
Lithiene Ribeiro
LR
Poi
Wilson Roberto
WR
D000086:Acetazolamide / Q000633:toxicity*; D000818:Animals; D004337:Drug Carriers; D008297:Male; D019946:Propylene Glycol; D051381:Rats; D017208:Rats, Wistar; D012388:Root Canal Irrigants / Q000633:toxicity*; D012391:Root Resorption / Q000517:prevention & control*; D012965:Sodium Chloride; D040521:Subcutaneous Tissue / Q000187:drug effects*; D014084:Tooth Avulsion / Q000601:surgery; D014090:Tooth Replantation
D004337:Drug Carriers; D012388:Root Canal Irrigants; D012965:Sodium Chloride; D019946:Propylene Glycol; D000086:Acetazolamide