title: Evaluation of real-time PCR method for rapid diagnosis of brucellosis with different clinical manifestations.
abstract: In this study, we tested the advantages of TaqMan real time PCR technique and compare it to conventional methods using serum samples from patients with different clinical forms of brucellosis. A total of 50 patients were included in the study. Blood culture using BACTEC 9240 system, Standard Wright's tube agglutination, and real time PCR methods were used. Control blood samples from 30 people with no history of brucellosis or exposure to Brucella spp. were examined too. Serological assay was positive for 49 patients (98%). Forty-four (88%) of the 50 patients had a positive PCR result, whereas Brucella spp were isolated from blood cultures of 18 patients (36%). STA test was all positive for focal brucellosis. Real time PCR test was positive in 9 patients with focal disease (90%), whereas blood culture was positive only in 4 patients (40%). The sensitivity, specificity, positive and negative predictive values of the real time PCR method were calculated as 88%, 100%, 100%, and 83%, respectively. Our results suggest that the high sensitivity and specificity of real time PCR method make it a useful tool for diagnosis of brucellosis with different clinical manifestations.
authors.affiliation: Celal Bayar University Medical Faculty, Department of Microbiology and Clinical Microbiology, Manisa, Turkey. surucuoglu@hotmail.com
authors.forename: Suheyla
authors.initials: S
authors.lastname: Surucuoglu
authors.forename: Sibel
authors.initials: S
authors.lastname: El
authors.forename: Serap
authors.initials: S
authors.lastname: Ural
authors.forename: Horu
authors.initials: H
authors.lastname: Gazi
authors.forename: Semra
authors.initials: S
authors.lastname: Kurutepe
authors.forename: Pinar
authors.initials: P
authors.lastname: Taskiran
authors.forename: Sureyya Gul
authors.initials: SG
authors.lastname: Yurtsever
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002002:Brucella / Q000235:genetics / Q000302:isolation & purification; D002006:Brucellosis / Q000097:blood / Q000150:complications / Q000175:diagnosis*; D002648:Child; D004269:DNA, Bacterial / Q000097:blood / Q000302:isolation & purification; D005260:Female; D006801:Humans; D008297:Male; D008581:Meningitis / Q000209:etiology; D008875:Middle Aged; D010019:Osteomyelitis / Q000209:etiology; D011237:Predictive Value of Tests; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*
chemical_list: D004269:DNA, Bacterial