title: RecX is involved in the switch between DNA damage response and normal metabolism in D. radiodurans.
abstract: Apart from inhibiting RecA activity through protein-protein interactions, Deinococcus radiodurans RecX inhibits the expression of RecA and two other anti-oxidant proteins. To identify the repertoire of proteins regulated by RecX, comparative proteomic studies were undertaken on a wild-type strain (R1) and recX null mutant (RecX(-)). Two-dimensional electrophoresis followed by MALDI-TOF identification revealed 35 differentially expressed proteins, including 12 up-regulated and 23 down-regulated proteins in the mutant. The 12 up-regulated proteins are DNA repair proteins, stress response proteins, and metabolism-related proteins. Most of these have been previously characterized as ionizing radiation-induced proteins. The 23 down-regulated proteins are mainly involved in cellular metabolism, and some of these are key enzymes in the metabolic pathway. Thus, RecX is suggested to be involved in the switch between DNA damage response and normal metabolism in D. radiodurans.
authors.affiliation: State Key Laboratory of Microbial Technology, Shandong University, 250100 Jinan, China. dhsheng@sdu.edu.cn
authors.forename: Duohong
authors.initials: D
authors.lastname: Sheng
authors.forename: Jiandong
authors.initials: J
authors.lastname: Jao
authors.forename: Mingfeng
authors.initials: M
authors.lastname: Li
authors.forename: Peng
authors.initials: P
authors.lastname: Xu
authors.forename: Junbing
authors.initials: J
authors.lastname: Zhang
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D004249:DNA Damage; D004260:DNA Repair / Q000502:physiology*; D045643:DNA Repair Enzymes / Q000235:genetics / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D034301:Deinococcus / Q000235:genetics / Q000378:metabolism*; D015536:Down-Regulation; D015180:Electrophoresis, Gel, Two-Dimensional; D015964:Gene Expression Regulation, Bacterial; D007525:Isoelectric Focusing; D040901:Proteomics; D011938:Rec A Recombinases / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D015854:Up-Regulation
chemical_list: D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; C430523:RecX protein, Xanthomonas campestris; D011938:Rec A Recombinases; D045643:DNA Repair Enzymes