Expression of angiogenesis-related genes in the cellular component of the blood of preeclamptic women.
The purpose of this study is to assess the changes in the expression of angiogenesis-related genes in the cellular component of the blood from preeclamptic patients. Blood samples were obtained from the preeclampsia (PE) and control participants. Cellular RNA was analyzed by reverse transcription polymerase chain reaction (PCR) to identify any angiogenesis-related genes and thereby assess the mRNA expression among women with PE and controls during weeks 35 to 41 of gestation. Significant differences were observed between PE and controls in all of the angiogenesis-related genes examined. In PE, for the increased expression of transforming growth factor-beta1 (TGF-beta1), endoglin and fms-like tyrosine kinase-1 (Flt-1); and the reduced expression of vascular endothelial growth factor (VEGF), placental growth factor (PlGF). fms-Like tyrosine kinase-1 and endoglin significantly correlated with the systolic pressure, while VEGF, Flt-1, and endoglin all correlated with proteinuria. An altered expression of angiogenesis-related genes was demonstrated in the cellular component of blood from preeclamptic patients. These findings indicate that this approach may offer an alternative way for evaluating the pathogenesis of PE.
Purwosunu
Yuditiya
Y
Department of Obstetrics and Gynecology, University of Indonesia, Cipto Mangunkusumo National Hospital, Jakarta, Indonesia.
Sekizawa
Akihiko
A
Yoshimura
Shiho
S
Farina
Antonio
A
Wibowo
Noroyono
N
Nakamura
Masamitsu
M
Shimizu
Hanako
H
Okai
Takashi
T
D000293:Adolescent; D000328:Adult; D015703:Antigens, CD / Q000235:genetics; D015415:Biomarkers; D001792:Blood Platelets / Q000502:physiology; D000071063:Endoglin; D005260:Female; D015870:Gene Expression / Q000502:physiology*; D017359:HELLP Syndrome / Q000235:genetics / Q000503:physiopathology; D006801:Humans; D009389:Neovascularization, Pathologic / Q000235:genetics* / Q000503:physiopathology*; D000072483:Placenta Growth Factor; D011225:Pre-Eclampsia / Q000235:genetics* / Q000503:physiopathology*; D011247:Pregnancy; D011257:Pregnancy Proteins / Q000235:genetics; D012333:RNA, Messenger / Q000378:metabolism; D011956:Receptors, Cell Surface / Q000235:genetics; D012720:Severity of Illness Index; D053773:Transforming Growth Factor beta1 / Q000235:genetics; D014327:Trophoblasts / Q000166:cytology / Q000502:physiology; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics; D040281:Vascular Endothelial Growth Factor Receptor-1 / Q000235:genetics; D055815:Young Adult
D015703:Antigens, CD; D015415:Biomarkers; C064206:ENG protein, human; D000071063:Endoglin; C000608036:PGF protein, human; D011257:Pregnancy Proteins; D012333:RNA, Messenger; D011956:Receptors, Cell Surface; D053773:Transforming Growth Factor beta1; C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A; D000072483:Placenta Growth Factor; C501162:FLT1 protein, human; D040281:Vascular Endothelial Growth Factor Receptor-1