[Proliferation effect of neural stem cell of ginsenoside Rg1 in vitro].
OBJECTIVE
To study the proliferation effect of neural stem cells (NSCs) of ginsenoside Rg1 in vitro.


METHODS
NSCs from the embryonic rat (E14) were isolated, 10 mg x L(-1) BrdU were added in the medium. The NSCs were incubated together with 1, 10 micromol x L(-1) ginsenoside Rg1 for 48 hours, control group were setup. Then BrdU positive cell number were detected and counted by fluorescence microscop. The Hes1 and Mash1 mRNA expression were detected by real-time RT-PCR.


RESULTS
The number of BrdU positive cells in 10 micromol x L(-1) ginsenoside Rg1 group was increased significantly compared with the control group (40.5 +/- 10.9 vs 26.2 +/- 6.0, P < 0.01), and the Hes1 mRNA expression were increased significantly (1.00 +/- 0.11) vs (1.28 +/- 0.14), P < 0.05.


CONCLUSIONS
ginsenoside Rg1 can promote the proliferation of neural stem cells in vitro, and this effect may be induced by the up-regulation Hes1 expression.
Zhuang
Pengwei
P
Tianjin University of Traditional Chinese Medicine, Tianjin Laboratory of Chinese Medience Pharmacology, Tianjin 300193, China. zhuangpengwei@163.com
Zhang
Yanjun
Y
Pang
Tan
T
D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects*; D036145:Ginsenosides / Q000494:pharmacology*; D039902:Multipotent Stem Cells / Q000187:drug effects / Q000502:physiology; D009474:Neurons / Q000187:drug effects* / Q000502:physiology; D051381:Rats; D017208:Rats, Wistar; D020133:Reverse Transcriptase Polymerase Chain Reaction
D036145:Ginsenosides; C035054:ginsenoside Rg1