title: [Molecular cloning and characterization of Ras-homologues from different developing stages of Echinococcus granulosus].
abstract: The Ras GTPase gene from protoscolex and adult worm of Echinococcus granulosus in Xinjiang were cloned by RT-PCR and named as Eg Ras-pro (GenBank No. EU560397) and Eg Ras-adult (GenBank No. EU560398). Sequence analysis showed that each gene had 552 bp, coding 184 aa with an pI of 6.54. Bioinformatics analysis revealed that Eg Ras-pro and Eg Ras-adult had 98.4% and 98.9% homology to Echinococcus multilocularis Ras and 53.9%-78.8% homology to other species. Phylogenetic analysis showed that Eg Ras-pro and Eg Ras-adult clustered with EmRas and SmRas. The data indicated that EgRas GTPase has been expressed from protoscolex and adult worm of Echinococcus granulosus, and both are highly conservative.
authors.affiliation: Key Laboratory of Xinjiang Hydatid Institute, The First Teaching Hospital of Xinjiang Medical University, Urumqi 830054, China.
authors.forename: Guo-dong
authors.initials: GD
authors.lastname: Lv
authors.forename: Jun-hua
authors.initials: JH
authors.lastname: Wang
authors.forename: Xiao-mei
authors.initials: XM
authors.lastname: Lu
authors.forename: Hao
authors.initials: H
authors.lastname: Wen
authors.forename: Ren-yong
authors.initials: RY
authors.lastname: Lin
mesh_terms: D000818:Animals; D003001:Cloning, Molecular; D048209:Echinococcus granulosus / Q000201:enzymology / Q000235:genetics*; D015801:Helminth Proteins / Q000235:genetics*; D020559:Monomeric GTP-Binding Proteins / Q000235:genetics*; D010802:Phylogeny; D017422:Sequence Analysis, DNA
chemical_list: D015801:Helminth Proteins; D020559:Monomeric GTP-Binding Proteins