Effect of N-acetylcysteine in attenuating ischemic reperfusion injury in patients undergoing coronary artery bypass grafting with cardiopulmonary bypass.
Ischemic reperfusion injury due to oxidative stress remains one of the challenging problems during cardiac surgeries. The imbalance in the production of free radicals and antioxidants in vivo determines the extent of oxidative stress. The use of antioxidants in cardioplegia has become an important strategy to salvage the myocardium from the attack of these radicals. The objective of this study was to analyze the cardioprotective effect of N-acetylcysteine (NAC) on early reperfusion injury in patients undergoing coronary artery bypass grafting using biochemical markers. Fifty-three patients with left ventricular ejection fraction >0.4 scheduled for coronary artery bypass grafting with cardiopulmonary bypass were selected and divided into two groups. The first group of patients (n=25) received isothermic cardioplegia alone, whereas the second group of patients (n=28) received cardioplegia enriched with NAC (50mg/kg body weight). The free radicals, antioxidants, cardiac troponin I, and hemodynamic and clinical properties of the patients were preoperatively and postoperatively evaluated at five different time intervals. Malondialdehyde level as a measure of free radicals was significantly lower in the NAC-enriched group during reperfusion (p<0.05) and after 12 hr (p<0.05) and 24hr (p<0.001) of surgery. All the antioxidants were elevated in the test group during the reperfusion period (p<0.01). A significant improvement (p=0.001) in the postoperative ejection fraction was noted in the test group. No significant differences were observed between the groups in the level of cardiac troponin I (p=not significant). The use of NAC in patients undergoing coronary artery bypass grafting using cardiopulmonary bypass decreased oxidative stress substantially. However, it did not lead to improvement in the level of cardiac troponin I, a marker of myocardial injury, in our study. Hence, the cardioprotective effect of NAC and the adaptation of the myocardium to oxidative stress should be extensively studied.
Prabhu
A
A
Department of Biochemistry, MMM Academy of Medical Sciences, Chennai, India. prabhu_bio@yahoo.com
Sujatha
D I
DI
Kanagarajan
N
N
Vijayalakshmi
M A
MA
Ninan
Benjamin
B
D000111:Acetylcysteine / Q000627:therapeutic use*; D000975:Antioxidants / Q000627:therapeutic use*; D015415:Biomarkers / Q000097:blood; D002315:Cardiopulmonary Bypass / Q000009:adverse effects*; D002316:Cardiotonic Agents / Q000627:therapeutic use*; D002374:Catalase / Q000097:blood; D001026:Coronary Artery Bypass / Q000009:adverse effects*; D004311:Double-Blind Method; D005978:Glutathione / Q000097:blood; D005979:Glutathione Peroxidase / Q000097:blood; D005980:Glutathione Reductase / Q000097:blood; D006324:Heart Arrest, Induced / Q000379:methods*; D006439:Hemodynamics / Q000187:drug effects; D006801:Humans; D008315:Malondialdehyde / Q000097:blood; D008875:Middle Aged; D015428:Myocardial Reperfusion Injury / Q000209:etiology / Q000378:metabolism / Q000503:physiopathology / Q000517:prevention & control*; D018384:Oxidative Stress / Q000187:drug effects*; D011446:Prospective Studies; D013318:Stroke Volume / Q000187:drug effects; D013482:Superoxide Dismutase / Q000097:blood; D013997:Time Factors; D016896:Treatment Outcome; D019210:Troponin I / Q000097:blood; D016277:Ventricular Function, Left / Q000187:drug effects
D000975:Antioxidants; D015415:Biomarkers; D002316:Cardiotonic Agents; D019210:Troponin I; D008315:Malondialdehyde; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D005980:Glutathione Reductase; D005978:Glutathione; D000111:Acetylcysteine