[Investigating the risk of two model virus in the laboratory].
OBJECTIVE
To study the survival time of recombination rival in environment and inactivation ability of different disinfectant and ultraviolet radiation against virus.


METHODS
NC membranes absorbed the recombinant adenovirus (rADV) or herpes simplex virus (rHSV) with green fluorescence protein (GFP) were laid, or immersed in various concentration of different disinfectants such as ethanol, sodium hypochlorite, lysol and geramine and then taked out them every 15 min, or exposed under ultraviolet radiation, then the NC membranes were adsorbed 1 h in cell, 37 degrees C 5% CO2 48 h. The results were observed under the fluorescence microscope.


RESULTS
(1) the average survival time of rHSV under environment is less than 60 min, rADV is almost up to 2 h. (2) The infection ability of rHSV and rADV was inactived 15 min by both ethanol (100%, 70% and 50%) and sodium hypochlorite (5%, 2.5% and 1.25%). (3) Two virus can be killed by 0.1% bromogeramine. (4) Both 5% and 2.5% lysol, but rADV can not lost the infection on Vero Cell until 75 min by 1.25% Lysol. (5) The rHSV was inactivated under ultraviolet radiation, but rADV was not.


CONCLUSIONS
The survival time of is different from both envelope rival and the no-envelope viral under nature environment and the inactivate ability of disinfectant also is different between two model virus; Disinfectant should be choose according to virus type.
Li
Yuan
Y
Medical School, Anhui University of Science & Technology, Huainan 232001, China.
Mei
Guo-yong
GY
Jiang
Hui-ying
HY
Wang
Gui-rong
GR
Tian
Chan
C
Han
Lu
L
Han
Wei-fang
WF
Wei
Qiang
Q
Zhou
Yong-yun
YY
Wu
Gui-zhen
GZ
Wang
Ke-xia
KX
Han
Jun
J
D000256:Adenoviridae / Q000187:drug effects / Q000528:radiation effects; D004202:Disinfectants / Q000633:toxicity*; D004203:Disinfection / Q000379:methods; D012306:Risk; D018139:Simplexvirus / Q000187:drug effects / Q000528:radiation effects; D012973:Sodium Hypochlorite / Q000633:toxicity; D013242:Sterilization / Q000379:methods; D014466:Ultraviolet Rays; D014777:Virus Diseases; D038301:Virus Inactivation; D018406:Virus Physiological Phenomena / Q000187:drug effects / Q000528:radiation effects; D014780:Viruses / Q000187:drug effects* / Q000528:radiation effects*
D004202:Disinfectants; D012973:Sodium Hypochlorite