Fragmin/protamine microparticle-coated matrix immobilized cytokines to stimulate various cell proliferations with low serum media.
Fragmin/protamine microparticles (F/P MPs) have been shown to bind to culture plates, thereby retaining heparin-binding cytokines. Most protocols for in vitro cultures of human microvascular endothelial cells (hMVECs), human dermal fibroblast cells (hDFCs), and hematopoietic cell line (TF-1) include high fetal bovine serum (FBS) (10%) medium as a nutritional supplement. Growth rates of those cells on the F/P MP-coated plates were higher in low FBS (1%) medium containing fibroblast growth factor (FGF)-2 (for hMVECs and hDFCs) and interleukin (IL)-3/granulocyte-macrophage colony-stimulating factor (for TF-1 cells) than without coating. The cytokines in low FBS medium were shown to be immobilized on the F/P MP-coated plate and released into the culture medium with a half releasing time of 4-5 days. Furthermore, those cells grew well on each cytokine-preimmobilized F/P MP-coated plate in low FBS medium. Thus, the F/P MP-coated matrix with adequate heparin-binding cytokines may provide biomaterials for controlling cellular growth and differentiation.
Kishimoto
Satoko
S
Research Institute, National Defense Medical College, Tokorozawa, Saitama, Japan.
Nakamura
Shingo
S
Nakamura
Shin-ichiro
S
Kanatani
Yasuhiro
Y
Hattori
Hidemi
H
Tanaka
Yoshihiro
Y
Harada
Yasuji
Y
Tagawa
Masahiro
M
Mori
Yasutaka
Y
Maehara
Tadaaki
T
Ishihara
Masayuki
M
D000818:Animals; D002417:Cattle; D018929:Cell Culture Techniques / Q000379:methods*; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D003470:Culture Media / Q000737:chemistry; D016207:Cytokines / Q000378:metabolism* / Q000494:pharmacology*; D017985:Dalteparin / Q000737:chemistry / Q000378:metabolism*; D016222:Fibroblast Growth Factor 2 / Q000378:metabolism / Q000494:pharmacology; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000378:metabolism / Q000494:pharmacology; D006801:Humans; D007377:Interleukin-3 / Q000378:metabolism / Q000494:pharmacology; D053758:Nanoparticles / Q000737:chemistry*; D011479:Protamines / Q000737:chemistry / Q000378:metabolism*; D011485:Protein Binding; D044967:Serum / Q000737:chemistry
D003470:Culture Media; D016207:Cytokines; D007377:Interleukin-3; D011479:Protamines; D016222:Fibroblast Growth Factor 2; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D017985:Dalteparin