title: The antihyperalgesic activity of a selective P2X7 receptor antagonist, A-839977, is lost in IL-1alphabeta knockout mice.
abstract: The pro-inflammatory cytokine interleukin-1beta (IL-1beta) has been implicated in both inflammatory processes and nociceptive neurotransmission. Activation of P2X7 receptors is the mechanism by which ATP stimulates the rapid maturation and release of IL-1beta from macrophages and microglial cells. Recently, selective P2X7 receptor antagonists have been shown to reduce inflammatory and neuropathic pain in animal models. However, the mechanisms underlying these analgesic effects are unknown. The present studies characterize the pharmacology and antinociceptive effects of a structurally novel P2X7 antagonist. A-839977 potently (IC50=20-150 nM) blocked BzATP-evoked calcium influx at recombinant human, rat and mouse P2X7 receptors. A-839977 also potently blocked agonist-evoked YO-PRO uptake and IL-1beta release from differentiated human THP-1 cells. Systemic administration of A-839977 dose-dependently reduced thermal hyperalgesia produced by intraplantar administration of complete Freund's adjuvant (CFA) (ED50=100 micromol/kg, i.p.) in rats. A-839977 also produced robust antihyperalgesia in the CFA model of inflammatory pain in wild-type mice (ED50=40 micromol/kg, i.p.), but the antihyperalgesic effects of A-839977 were completely absent in IL-1alphabeta knockout mice. These data demonstrate that selective blockade of P2X7 receptors in vivo produces significant antinociception in animal models of inflammatory pain and suggest that the antihyperalgesic effects of P2X7 receptor blockade in an inflammatory pain model in mice are mediated by blocking the release of IL-1beta.
authors.affiliation: Abbott Laboratories, Neuroscience Research, Abbott Park, IL 60064, USA.
authors.forename: Prisca
authors.initials: P
authors.lastname: Honore
authors.forename: Diana
authors.initials: D
authors.lastname: Donnelly-Roberts
authors.forename: Marian
authors.initials: M
authors.lastname: Namovic
authors.forename: Chengmin
authors.initials: C
authors.lastname: Zhong
authors.forename: Carrie
authors.initials: C
authors.lastname: Wade
authors.forename: Prasant
authors.initials: P
authors.lastname: Chandran
authors.forename: Chang
authors.initials: C
authors.lastname: Zhu
authors.forename: William
authors.initials: W
authors.lastname: Carroll
authors.forename: Arturo
authors.initials: A
authors.lastname: Perez-Medrano
authors.forename: Yoichiro
authors.initials: Y
authors.lastname: Iwakura
authors.forename: Michael F
authors.initials: MF
authors.lastname: Jarvis
mesh_terms: D000255:Adenosine Triphosphate / Q000031:analogs & derivatives / Q000378:metabolism; D018712:Analgesics, Non-Narcotic / Q000737:chemistry / Q000494:pharmacology*; D000818:Animals; D002118:Calcium / Q000378:metabolism; D002460:Cell Line; D004305:Dose-Response Relationship, Drug; D005620:Freund's Adjuvant; D006358:Hot Temperature; D006801:Humans; D053582:Interleukin-1alpha / Q000235:genetics / Q000378:metabolism*; D053583:Interleukin-1beta / Q000235:genetics / Q000378:metabolism*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D018345:Mice, Knockout; D010146:Pain / Q000139:chemically induced / Q000188:drug therapy* / Q000378:metabolism; D058919:Purinergic P2 Receptor Antagonists; D011725:Pyridines / Q000737:chemistry / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D018048:Receptors, Purinergic P2 / Q000096:biosynthesis / Q000378:metabolism*; D058486:Receptors, Purinergic P2X7; D011994:Recombinant Proteins / Q000037:antagonists & inhibitors / Q000096:biosynthesis / Q000378:metabolism; D013777:Tetrazoles / Q000737:chemistry / Q000494:pharmacology*
chemical_list: C545178:A-839977; D018712:Analgesics, Non-Narcotic; D053582:Interleukin-1alpha; D053583:Interleukin-1beta; C546468:P2RX7 protein, human; C546469:P2rx7 protein, mouse; C546470:P2rx7 protein, rat; D058919:Purinergic P2 Receptor Antagonists; D011725:Pyridines; D018048:Receptors, Purinergic P2; D058486:Receptors, Purinergic P2X7; D011994:Recombinant Proteins; D013777:Tetrazoles; C033901:3'-O-(4-benzoyl)benzoyladenosine 5'-triphosphate; D000255:Adenosine Triphosphate; D005620:Freund's Adjuvant; D002118:Calcium