title: Expression of gonadotropin alpha, follicle-stimulating hormone beta, and luteinizing hormone beta genes of the hermaphroditic fish Kryptolebias marmoratus exposed to octylphenol, 17beta estradiol, and tamoxifen.
abstract: In order to study the expression of the gonadotropin alpha subunit and beta subunit [follicle-stimulating hormone (FSH)-beta and luteinizing hormone (LH)-beta] cDNAs in the hermaphroditic fish Kryptolebias marmoratus, we cloned these genes and analyzed their modulatory effects in the brain/pituitary tissue after exposure to 17beta estradiol (E2), tamoxifen (TMX), and octyphenol. Gonadotropin beta subunit (FSH-beta and LH-beta) showed E2-dependent upregulation in most hermaphrodites, whereas TMX did not show elevated expression. Octyphenol also showed a similar effect of upregulation on FSH-beta expression but not for the LH-beta gene. These results suggest that the expression of FSH-beta and LH-beta in K. marmoratus is associated with their expression on endocrine-disrupting chemicals in this species.
authors.affiliation: Department of Molecular and Environmental Bioscience, Graduate School, Hanyang University, Seoul, Korea.
authors.forename: Jae-Sung
authors.initials: JS
authors.lastname: Rhee
authors.forename: Young-Mi
authors.initials: YM
authors.lastname: Lee
authors.forename: Jung Soo
authors.initials: JS
authors.lastname: Seo
authors.forename: JeongHoon
authors.initials: J
authors.lastname: Han
authors.forename: Jae-Seong
authors.initials: JS
authors.lastname: Lee
mesh_terms: D000818:Animals; D003532:Cyprinodontiformes / Q000235:genetics*; D004958:Estradiol / Q000494:pharmacology*; D037201:Follicle Stimulating Hormone, beta Subunit / Q000235:genetics*; D005786:Gene Expression Regulation / Q000187:drug effects / Q000235:genetics; D006062:Gonadotropins / Q000235:genetics*; D037101:Luteinizing Hormone, beta Subunit / Q000235:genetics*; D010636:Phenols / Q000494:pharmacology*; D013629:Tamoxifen / Q000494:pharmacology*
chemical_list: D037201:Follicle Stimulating Hormone, beta Subunit; D006062:Gonadotropins; D037101:Luteinizing Hormone, beta Subunit; D010636:Phenols; C474055:octylphenol; D013629:Tamoxifen; D004958:Estradiol