[The usefulness of CD203c expression measurement on basophils after activation with grass pollen and Dermatophagoides pteronyssinus antigens. Preliminary study].
BACKGROUND
'Gold standard' in the diagnosis of atopic disease are skin prick tests and specific IgE evaluation. Well-established in vitro tests, such as the histamine release test, the leukotriens release test and the flow cytometric basophil activation test can be very helpful in diagnostics, especially when the skin prick test is contraindicated. The aim of this study was to evaluate the usefulness of antigen CD203c expression, as a marker of basophil activation by grass pollen or D. pteronyssinus antigens.


METHODS
Peripheral blood from 13 allergic patients and nine healthy volunteers was analysed. Basophils activation was measured by the breakdown of antigen CD203c expression with Allergenicity Kit (Beckman Coulter), using Cytomics FC 500 flow cytometer (Beckman Coulter).


RESULTS
The sensitivity was 92.3% and specificity of test was 100%. 50.95 +/- 15.7% of basophils (median 49.7%, 1.91-72.42%) were activated after grass pollen stimulation in atopic patients sensitised to this allergen, in comparison to 1.91% (0.00-7.96%) in control patients (p = 0.002). The percentage of activated basophils after D. pteronyssinus antigens stimulation was 40.6 +/- 25.2% in allergic patients, compared to only 2.51 +/- 1 96% of basophils from non-atopic controls (p = 0.0003). Basophils from 21 patients responded after anti-IgE stimulation (44.1 +/- 18.9%), and none of the analysed samples was activated after PBS stimulation (2.03 +/- 1.19%, p < 0.0001).


CONCLUSIONS
These results demonstrate that basophil activation test based on antigen CD203c expression is very accurate in the diagnosis of atopic diseases.
Potapińska
Olga
O
Zakład Diagnostyki Laboratoryjnej i Immunologii Klinicznej Wieku Rozwojowego Warszawskiego Uniwersytetu Medycznego. olga.potapinska@wum.edu.pl
Górska
Elzbieta
E
Zawadzka-Krajewska
Anna
A
Kulus
Marek
M
Wasik
Maria
M
Demkow
Urszula
U
D000293:Adolescent; D000485:Allergens / Q000276:immunology*; D000818:Animals; D039741:Antigens, Dermatophagoides / Q000097:blood*; D015549:Basophil Degranulation Test / Q000379:methods; D001491:Basophils / Q000276:immunology*; D015415:Biomarkers / Q000097:blood; D002648:Child; D040002:Dermatophagoides pteronyssinus / Q000276:immunology*; D004305:Dose-Response Relationship, Drug; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D008297:Male; D010727:Phosphoric Diester Hydrolases / Q000097:blood*; D011058:Pollen / Q000276:immunology; D011755:Pyrophosphatases / Q000097:blood*; D012221:Rhinitis, Allergic, Perennial / Q000276:immunology*; D012680:Sensitivity and Specificity
D000485:Allergens; D039741:Antigens, Dermatophagoides; D015415:Biomarkers; C427944:ENPP3 protein, human; D010727:Phosphoric Diester Hydrolases; D011755:Pyrophosphatases