[Study on relationship between hepatitis B virus DNA load and genotype with large envelope protein].
OBJECTIVE
To explore the relation between hepatitis B virus DNA load and genotype with the level of large envelope protein.


METHODS
Serum HBV DNA was quantitively detected by using real time polymerase chain reaction (RT-PCR). The LHBs were detected by using enzyme linked immuno sorbent assay (ELISA) and HBV markers were detected by time differentiate immunofluorescence assay in 140 serum samples collected from chronic hepatitis B patients.The genotypes of HBV were identified by DNA sequencing; and analyze their relationship.


RESULTS
There was no significant difference between positive rate of LHBs and that of HBV DNA in HBeAg negative and positive group (P > 0.05); The HBV LHBs absorbency was markedly correlated with the HBV DNA load ( R2 = 0.9267). The difference of HBV LHBs absorbency between HBV genotype B and C was not significant.


CONCLUSIONS
The close correlation between HBV LHBs absorbence and HBV DNA load illustrated that he level of serum LHBs can be used to estimate the state of HBV replication; and there is no relationship between HBV LHBs absorbency and genotypes. So HBV LHBs may be used as a new serological marker to detect HBV replication.
Rao
Gao-feng
GF
Wenling Hospital, Wenzhou Medical College, Wenzhou 317500, China. raogf@163.com
Chen
En-fu
EF
Yan
Ming-he
MH
Zheng
Min-qiao
MQ
D000293:Adolescent; D000328:Adult; D000368:Aged; D004279:DNA, Viral / Q000032:analysis* / Q000235:genetics / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005838:Genotype; D006509:Hepatitis B / Q000235:genetics / Q000821:virology; D006515:Hepatitis B virus / Q000737:chemistry / Q000235:genetics*; D006801:Humans; D008297:Male; D008875:Middle Aged; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014759:Viral Envelope Proteins / Q000737:chemistry / Q000235:genetics; D014771:Virion / Q000737:chemistry / Q000235:genetics*; D055815:Young Adult
D004279:DNA, Viral; D014759:Viral Envelope Proteins