title: Platelet-rich plasma and platelet-rich fibrin in human cell culture.
abstract: OBJECTIVE
  The clinical use of platelet-rich plasma (PRP) for preprosthetic surgery has been a matter of controversy until now. Only recently, a new blood preparation has been developed which results in platelet-rich fibrin (PRF). The objective of the present investigation was to examine the growth factor release from PRP and PRF in vitro.
  
  
  METHODS
  Whole blood samples from healthy participants (n = 10) were drawn to generate PRP and PRF. Human osteoblasts (O), human fibroblasts (F), and human osteoblast-derived osteosarcoma cells (Saos-2) were used for the cell culture. Cells of each cell line were cultivated, and PRP- or PRF-preparations added for ten days. The drawn medium was pooled and the quantities of growth factors (platelet-derived growth factor isomers AB and BB, insulin-like growth factor I, and transforming growth factor (TGF) isomers beta1 and beta2) analyzed by enzyme-linked immunosorbent assay.
  
  
  RESULTS
  In osteoblast and Saos-2 cultures, cytokine concentrations were significantly higher for PRP than for PRF (P < .05). In fibroblast cultures, results were the same with the exception of TGF-beta2 (P < .05).
  
  
  CONCLUSIONS
  This study demonstrates that PRP application in cell cultures leads to higher levels of growth factors than PRF application.
authors.affiliation: Department of Oral and Maxillofacial Surgery, University Hospital of Schleswig-Holstein, Campus Kiel, Kiel, Germany. gassling@mkg.uni-kiel.de
authors.forename: Volker L W
authors.initials: VL
authors.lastname: Gassling
authors.forename: Yahya
authors.initials: Y
authors.lastname: Açil
authors.forename: Ingo N
authors.initials: IN
authors.lastname: Springer
authors.forename: Nina
authors.initials: N
authors.lastname: Hubert
authors.forename: Jörg
authors.initials: J
authors.lastname: Wiltfang
mesh_terms: D000328:Adult; D000077214:Becaplermin; D001792:Blood Platelets / Q000502:physiology*; D002460:Cell Line; D045744:Cell Line, Tumor; D003470:Culture Media; D005337:Fibrin / Q000502:physiology*; D005347:Fibroblasts / Q000502:physiology; D006801:Humans; D007334:Insulin-Like Growth Factor I / Q000032:analysis / Q000378:metabolism; D036341:Intercellular Signaling Peptides and Proteins / Q000032:analysis / Q000378:metabolism*; D008422:Materials Testing; D008875:Middle Aged; D010006:Osteoblasts / Q000502:physiology; D012516:Osteosarcoma / Q000473:pathology; D010982:Platelet-Derived Growth Factor / Q000032:analysis / Q000378:metabolism; D053657:Platelet-Rich Plasma / Q000502:physiology*; D020574:Proto-Oncogene Proteins c-sis; D053773:Transforming Growth Factor beta1 / Q000032:analysis / Q000378:metabolism; D053781:Transforming Growth Factor beta2 / Q000032:analysis / Q000378:metabolism
chemical_list: D003470:Culture Media; D036341:Intercellular Signaling Peptides and Proteins; D010982:Platelet-Derived Growth Factor; D020574:Proto-Oncogene Proteins c-sis; D053773:Transforming Growth Factor beta1; D053781:Transforming Growth Factor beta2; C095477:platelet-derived growth factor AB; D000077214:Becaplermin; D007334:Insulin-Like Growth Factor I; D005337:Fibrin