title: Serglycin proteoglycan is not implicated in localizing exocrine pancreas enzymes to zymogen granules.
abstract: Storage and release of proteins from granules forms the basis of cellular functions as diverse as cell mediated cytotoxicity, neuronal communication, activation of muscle fibres, and release of hormones or digestive enzymes from endocrine and exocrine glands, such as the pancreas. Serglycin is the major intracellular proteoglycan of haematopoietic cells. Serglycin is important for localization of proteins in granules of different haematopoietic cell types. Previous reports have indicated a role for serglycin in granule formation and localization of zymogens in granules of the exocrine pancreas in rat. We here present data showing that serglycin is not present at the protein level in human or murine pancreas. Furthermore, the amount and localization of three exocrine pancreas zymogens (amylase, trypsinogen, and carboxypeptidase A) is not affected by the absence of serglycin in a serglycin knock-out mouse model.
authors.affiliation: Department of Haematology, Rigshospitalet, The Granulocyte Research laboratory, Building 9322, Blegdamsvej 9, DK-2100 Copenhagen, Denmark. niemann@dadlnet.dk
authors.forename: Carsten U
authors.initials: CU
authors.lastname: Niemann
authors.forename: Jack B
authors.initials: JB
authors.lastname: Cowland
authors.forename: Elisabeth
authors.initials: E
authors.lastname: Ralfkiaer
authors.forename: Magnus
authors.initials: M
authors.lastname: Abrink
authors.forename: Gunnar
authors.initials: G
authors.lastname: Pejler
authors.forename: Niels
authors.initials: N
authors.lastname: Borregaard
mesh_terms: D000681:Amylases / Q000378:metabolism; D000818:Animals; D043422:Carboxypeptidases A / Q000378:metabolism; D004792:Enzyme Precursors / Q000378:metabolism*; D006801:Humans; D051379:Mice; D010179:Pancreas / Q000166:cytology / Q000201:enzymology*; D011509:Proteoglycans / Q000032:analysis / Q000502:physiology*; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D022142:Secretory Vesicles / Q000378:metabolism*; D014362:Trypsinogen / Q000378:metabolism; D033921:Vesicular Transport Proteins / Q000032:analysis / Q000502:physiology*
chemical_list: D004792:Enzyme Precursors; D011509:Proteoglycans; D012333:RNA, Messenger; D033921:Vesicular Transport Proteins; C075430:serglycin; D014362:Trypsinogen; D000681:Amylases; D043422:Carboxypeptidases A