title: Immunohistochemical analysis for CD21, CD35, Caldesmon and S100 protein on dendritic cells types in oral lymphomas.
abstract: OBJECTIVE
  Follicular dendritic cells (FDCs) and interdigitating dendritic cells (IDCs) are dendritic cells found in lymphoid follicles, reactive follicles and in lymphomas. The goal of this study was to evaluate the presence and distribution of FDCs and IDCs in oral lymphomas.
  
  
  METHODS
  Immunohistochemistry reactions were applied to 50 oral lymphomas using the antibodies anti-CD21, anti-CD35 and anti-caldesmon to FDCs, and anti-S100 protein to IDCs. Caldesmon+/FDCs and S100+/IDCs were quantified in Imagelab software.
  
  
  RESULTS
  FDCs revealed by CD21 and CD35 were positively stained in two cases of diffuse large B-cell lymphoma, one MALT lymphoma, and in one case of mantle cell lymphoma. FDCs were immunopositive to caldesmon in all cases, as well as IDCs to S100 protein. Burkitt lymphoma presented a lower amount of caldesmon+/FDCs and S100+/IDCs than diffuse large B-cell lymphoma and plasmablastic lymphoma of the oral mucosa type.
  
  
  CONCLUSIONS
  The microenvironment determined by neoplastic lymphoid cells in oral lymphomas is responsible by the development and expression of dendritic cells types.
authors.affiliation: Department of Oral Surgery, Oral Medicine and Oral Pathology, Dental School, Federal University of Minas Gerais, Belo Horizonte, MG, Brazil. ramesquita@ufmg.br
authors.forename: Ricardo Alves
authors.initials: RA
authors.lastname: Mesquita
authors.forename: Vera Cavalcanti
authors.initials: VC
authors.lastname: de Araújo
authors.forename: Roberto Antônio Pinto
authors.initials: RA
authors.lastname: Paes
authors.forename: Fábio Daumas
authors.initials: FD
authors.lastname: Nunes
authors.forename: Suzana Cantanhede Orsini Machado
authors.initials: SC
authors.lastname: de Souza
mesh_terms: D002148:Calmodulin-Binding Proteins / Q000032:analysis; D003713:Dendritic Cells / Q000737:chemistry*; D020566:Dendritic Cells, Follicular / Q000737:chemistry*; D006801:Humans; D007150:Immunohistochemistry; D018442:Lymphoma, B-Cell, Marginal Zone / Q000737:chemistry / Q000473:pathology; D016403:Lymphoma, Large B-Cell, Diffuse / Q000737:chemistry / Q000473:pathology; D020522:Lymphoma, Mantle-Cell / Q000737:chemistry / Q000473:pathology; D008228:Lymphoma, Non-Hodgkin / Q000737:chemistry* / Q000473:pathology; D009062:Mouth Neoplasms / Q000737:chemistry* / Q000473:pathology; D017463:Receptors, Complement 3b / Q000032:analysis; D017464:Receptors, Complement 3d / Q000032:analysis; D009418:S100 Proteins / Q000032:analysis
chemical_list: D002148:Calmodulin-Binding Proteins; D017463:Receptors, Complement 3b; D017464:Receptors, Complement 3d; D009418:S100 Proteins