Transcriptome and proteome analysis of ovaries of arrhenotokous and thelytokous Venturia canescens.
Under arrhenotoky, unfertilized haploid eggs develop as males but under thelytoky they develop into diploid females after they have undergone diploidy restoration. In the parasitoid wasp Venturia canescens both reproductive modes occur. Thelytoky is genetically determined but the underlying genetics of diploidy restoration remain unknown. In this study we aim to identify the genes and/or proteins that control thelytoky. cDNA-amplified fragment length polymorphism (cDNA-AFLP) analysis of total ovarian RNA and two-dimensional protein electrophoresis in combination with mass spectrometry revealed putative transcripts and proteins involved in arrhenotokous and thelytokous development. The detected tubulin and actin protein differences are most likely functionally related to the two types of reproduction.
Mateo Leach
I
I
Evolutionary Genetics, Center for Ecological and Evolutionary Studies, University of Groningen, NL-9750 AA Haren, The Netherlands. i.mateo-leach@rug.nl
Hesseling
A
A
Huibers
W H C
WH
Witsenboer
H
H
Beukeboom
L W
LW
van de Zande
L
L
D000818:Animals; D005260:Female; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000502:physiology*; D006927:Hymenoptera / Q000235:genetics / Q000378:metabolism*; D019476:Insect Proteins / Q000235:genetics / Q000378:metabolism; D008297:Male; D010053:Ovary / Q000378:metabolism*; D019849:Sex Determination Processes
D019476:Insect Proteins