Acetic acid activates PKD1L3-PKD2L1 channel--a candidate sour taste receptor.
The polycystic kidney disease (PKD) 1L3-PKD2L1 channel is a candidate sour taste receptor expressed in mammalian taste receptor cells. Various acids are reported to activate PKD channels after the removal of the acid stimuli, but little information is available on the activation of these channels by acetic acid. It was difficult to analyze the PKD channel activation by acetic acid using Ca2+ imaging experiments because this acid induces a transient and nonspecific response in cultured cells. Here, we developed a novel method to evaluate PKD channel activation by acetic acid. Nonspecific responses were observed only over a short period after the application of acetic acid. In contrast, PKD channel activation evoked by acetic acid as well as citric acid was detected even at a later time point. This method revealed that PKD1L3-PKD2L1 channel activation by acetic acid was pH-dependent and occurred when the ambient pH was <3.1.
Ishii
Sho
S
Central Research Institute, Mizkan Group Co., 2-6 Nakamura-cho, Handa, Aichi 475-8585, Japan.
Misaka
Takumi
T
Kishi
Mikiya
M
Kaga
Takayuki
T
Ishimaru
Yoshiro
Y
Abe
Keiko
K
D019342:Acetic Acid / Q000494:pharmacology*; D000818:Animals; D002118:Calcium / Q000378:metabolism; D015220:Calcium Channels; D002460:Cell Line; D019343:Citric Acid / Q000494:pharmacology; D006801:Humans; D051379:Mice; D011956:Receptors, Cell Surface / Q000819:agonists*; D050396:TRPP Cation Channels / Q000819:agonists*; D013649:Taste
D015220:Calcium Channels; C512453:PKD1L3 protein, mouse; C486487:Pkd2l1 protein, mouse; D011956:Receptors, Cell Surface; D050396:TRPP Cation Channels; D019343:Citric Acid; D019342:Acetic Acid; D002118:Calcium