title: [Antimicrobial resistance of clinical isolates of Stenotrophomonas maltophilia].
abstract: OBJECTIVE
  To investigate the antimicrobial resistance of clinical isolates of Stenotrophomonas matophilia (SMA) and the mechanisms of their drug resistance.
  
  
  METHODS
  Disc diffusion method (NCCLS) was used to detect the resistant patterns of 88 initial SMA isolates resistant to 12 antibiotics isolated from a local hospital in the past 4 years. PCR was used to detect the 7 aminoglycosides modifying enzymes genes (AME) against amikacin and gentamicin. Metal-beta-lactamases (MBLs) were screened by synergic method, and extended-spectrum beta-lactamases (ESBLs) were detected by double-disk synergy test.
  
  
  RESULTS
  The resistance rates of the SMA isolates were 0%-9.7% to minocycline, 12.5%-22.6% to ticarcillin-clavulanic acid, 12.5%-28.6% to levofloxacin, 18.8%-33.3% to doxycycline, 18.8%-40% to sulfamethoxazole compound, 50%-65.7% to ciprofloxacin, 50%-66.7% to cehazindme, 54.8%-66.7% to amikacin, 75%-100% to gentamicin, 81.3%-100% to piperacillin, 87.5%-100% to aztreonam and 93.5%-100% to imipenem. Aac(3)-I and ant(4')-II were not detected in these strains. The positive rates of the other 5 AME genes of aac(3)-II, ant(2'')-I, aac(6')-I, aac(3)-III, aac(3)-IV were 2.3%, 5.7%, 8%, 10%, and 10%, respectively. SMA strains producing ESBLs were found at the rate of 38.6%; 25% of the strains were MBL-producing, and 13.6% produced both ESBLs and MBLs.
  
  
  CONCLUSIONS
  Most of the SMAs we isolated are multidrug-resistant through various mechanisms. The choice of antibiotics should be made according to the susceptibility results.
authors.affiliation: Department of Epidemiology, School of Public Health and Tropical Medicine, Southern Medical University, and Clinical Laboratory, Guangzhou First People's Hospital, Guangzhou 510515, China.
authors.forename: Zu-qiong
authors.initials: ZQ
authors.lastname: Hu
authors.forename: Yin-mei
authors.initials: YM
authors.lastname: Yang
authors.forename: Xue-mei
authors.initials: XM
authors.lastname: Ke
authors.forename: Xu-qi
authors.initials: XQ
authors.lastname: Ren
authors.forename: Wen
authors.initials: W
authors.lastname: Zhou
authors.forename: Qing
authors.initials: Q
authors.lastname: Chen
authors.forename: Jing
authors.initials: J
authors.lastname: Hu
authors.forename: Shou-yi
authors.initials: SY
authors.lastname: Yu
mesh_terms: D000583:Amikacin / Q000494:pharmacology*; D024901:Drug Resistance, Multiple, Bacterial; D005839:Gentamicins / Q000494:pharmacology*; D006801:Humans; D015378:Imipenem / Q000494:pharmacology*; D008826:Microbial Sensitivity Tests; D020615:Stenotrophomonas maltophilia / Q000187:drug effects* / Q000302:isolation & purification
chemical_list: D005839:Gentamicins; D015378:Imipenem; D000583:Amikacin