title: Differential effects of a transgene to confer low phytic acid in caryopses located at different positions in rice panicles.
abstract: In previous studies, we attempted to reduce phytic acid in rice seeds by silencing the 1d-myo-inositol 3-phosphate synthase gene, RINO1, using an antisense sequence under the control of the rice glutelin GluB-1 promoter. The stable transgenic line showed a weak low phytic acid phenotype. In this study, we show that the position of the caryopsis in the panicle might affect the level of gene silencing through a difference in temporal and spatial expression patterns between RINO1 and GluB-1 promoters, resulting in a large variation in Pi levels and a small increase in Pi in the transgenic seeds.
authors.affiliation: Graduate School of Agricultural and Life Sciences, University of Tokyo, Bunkyo-ku, Tokyo, Japan.
authors.forename: Mio
authors.initials: M
authors.lastname: Kuwano
authors.forename: Fumio
authors.initials: F
authors.lastname: Takaiwa
authors.forename: Kaoru T
authors.initials: KT
authors.lastname: Yoshida
mesh_terms: D018506:Gene Expression Regulation, Plant; D020868:Gene Silencing; D005983:Glutens / Q000235:genetics / Q000378:metabolism; D019753:Intramolecular Lyases / Q000235:genetics / Q000378:metabolism*; D012275:Oryza / Q000201:enzymology / Q000235:genetics*; D010710:Phosphates / Q000378:metabolism; D010758:Phosphorus / Q000378:metabolism; D010833:Phytic Acid / Q000096:biosynthesis*; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D030821:Plants, Genetically Modified / Q000235:genetics / Q000378:metabolism; D011401:Promoter Regions, Genetic; D012639:Seeds / Q000235:genetics / Q000254:growth & development; D019076:Transgenes
chemical_list: C120054:GluB-1 protein, Oryza; D010710:Phosphates; D010940:Plant Proteins; D010758:Phosphorus; D010833:Phytic Acid; D005983:Glutens; D019753:Intramolecular Lyases; C105497:D-myo-inositol-3-phosphate synthase