title: Remorin, a solanaceae protein resident in membrane rafts and plasmodesmata, impairs potato virus X movement.
abstract: Remorins (REMs) are proteins of unknown function specific to vascular plants. We have used imaging and biochemical approaches and in situ labeling to demonstrate that REM clusters at plasmodesmata and in approximately 70-nm membrane domains, similar to lipid rafts, in the cytosolic leaflet of the plasma membrane. From a manipulation of REM levels in transgenic tomato (Solanum lycopersicum) plants, we show that Potato virus X (PVX) movement is inversely related to REM accumulation. We show that REM can interact physically with the movement protein TRIPLE GENE BLOCK PROTEIN1 from PVX. Based on the localization of REM and its impact on virus macromolecular trafficking, we discuss the potential for lipid rafts to act as functional components in plasmodesmata and the plasma membrane.
authors.affiliation: Laboratoire de Biogenèse Membranaire, Unité Mixte de Recherche 5200, Centre National de la Recherche Scientifique-University of Bordeaux, Bordeaux 33076, France.
authors.forename: Sylvain
authors.initials: S
authors.lastname: Raffaele
authors.forename: Emmanuelle
authors.initials: E
authors.lastname: Bayer
authors.forename: David
authors.initials: D
authors.lastname: Lafarge
authors.forename: Stéphanie
authors.initials: S
authors.lastname: Cluzet
authors.forename: Sylvie
authors.initials: S
authors.lastname: German Retana
authors.forename: Tamy
authors.initials: T
authors.lastname: Boubekeur
authors.forename: Nathalie
authors.initials: N
authors.lastname: Leborgne-Castel
authors.forename: Jean-Pierre
authors.initials: JP
authors.lastname: Carde
authors.forename: Jeannine
authors.initials: J
authors.lastname: Lherminier
authors.forename: Elodie
authors.initials: E
authors.lastname: Noirot
authors.forename: Béatrice
authors.initials: B
authors.lastname: Satiat-Jeunemaître
authors.forename: Jeanny
authors.initials: J
authors.lastname: Laroche-Traineau
authors.forename: Patrick
authors.initials: P
authors.lastname: Moreau
authors.forename: Thomas
authors.initials: T
authors.lastname: Ott
authors.forename: Andrew J
authors.initials: AJ
authors.lastname: Maule
authors.forename: Philippe
authors.initials: P
authors.lastname: Reymond
authors.forename: Françoise
authors.initials: F
authors.lastname: Simon-Plas
authors.forename: Edward E
authors.initials: EE
authors.lastname: Farmer
authors.forename: Jean-Jacques
authors.initials: JJ
authors.lastname: Bessoule
authors.forename: Sébastien
authors.initials: S
authors.lastname: Mongrand
mesh_terms: D002352:Carrier Proteins / Q000032:analysis / Q000378:metabolism / Q000502:physiology*; D002458:Cell Fractionation; D049452:Green Fluorescent Proteins / Q000032:analysis; D007113:Immunity, Innate; D018551:Solanum lycopersicum / Q000378:metabolism / Q000648:ultrastructure / Q000821:virology*; D021962:Membrane Microdomains / Q000378:metabolism*; D008969:Molecular Sequence Data; D010750:Phosphoproteins / Q000032:analysis / Q000378:metabolism / Q000502:physiology*; D010935:Plant Diseases / Q000821:virology; D010940:Plant Proteins / Q000032:analysis / Q000378:metabolism / Q000502:physiology*; D030821:Plants, Genetically Modified / Q000821:virology; D031425:Plasmodesmata / Q000378:metabolism*; D017863:Potexvirus / Q000502:physiology*; D011993:Recombinant Fusion Proteins / Q000032:analysis; D014779:Virus Replication
chemical_list: D002352:Carrier Proteins; D010750:Phosphoproteins; D010940:Plant Proteins; D011993:Recombinant Fusion Proteins; C103794:remorin; D049452:Green Fluorescent Proteins