title: Ectopic expression of Arabidopsis ABC transporter MRP7 modifies cadmium root-to-shoot transport and accumulation.
abstract: Arabidopsis MRPs/ABCCs have been shown to remove various organic and inorganic substrates from the cytosol to other subcellular compartments. Here we first demonstrate that heterologous expression of AtMRP7 in tobacco (Nicotiana tabacum var. Xanthi) modifies cadmium accumulation, distribution and tolerance. Arabidopsis MRP7 was localized both in the tonoplast and in the plasma membrane when expressed in tobacco. Its overexpression increased tobacco Cd-tolerance and resulted in enhanced cadmium concentration in leaf vacuoles, indicating more efficient detoxification by means of vacuolar storage. Heterologous AtMRP7 expression also led to more efficient retention of Cd in roots, suggesting a contribution to the control of cadmium root-to-shoot translocation. The results underscore the use of AtMRP7 in plant genetic engineering to modify the heavy-metal accumulation pattern for a broad range of applications.
authors.affiliation: Faculty of Biology, University of Warsaw, Miecznikowa str. 1, 02-096 Warszawa, Poland.
authors.forename: Sylwia
authors.initials: S
authors.lastname: Wojas
authors.forename: Jacek
authors.initials: J
authors.lastname: Hennig
authors.forename: Sonia
authors.initials: S
authors.lastname: Plaza
authors.forename: Markus
authors.initials: M
authors.lastname: Geisler
authors.forename: Oskar
authors.initials: O
authors.lastname: Siemianowski
authors.forename: Aleksandra
authors.initials: A
authors.lastname: Skłodowska
authors.forename: Anna
authors.initials: A
authors.lastname: Ruszczyńska
authors.forename: Ewa
authors.initials: E
authors.lastname: Bulska
authors.forename: Danuta M
authors.initials: DM
authors.lastname: Antosiewicz
mesh_terms: D018528:ATP-Binding Cassette Transporters / Q000235:genetics / Q000378:metabolism*; D017360:Arabidopsis / Q000235:genetics* / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000235:genetics* / Q000378:metabolism; D001692:Biological Transport; D002104:Cadmium / Q000378:metabolism*; D015870:Gene Expression; D018517:Plant Roots / Q000235:genetics / Q000378:metabolism*; D018520:Plant Shoots / Q000235:genetics / Q000378:metabolism*; D014026:Nicotiana / Q000235:genetics / Q000378:metabolism
chemical_list: D018528:ATP-Binding Cassette Transporters; D029681:Arabidopsis Proteins; D002104:Cadmium