title: Factors effecting expression of vaccines in microalgae.
abstract: PhycoBiologics is developing an oral vaccine delivery system using vaccines expressed in the chloroplast of microalgae. Despite many advances in plastid transformation technology, levels of expression remain inconsistent. We have concluded that the main factors affecting the level of recombinant protein expression in the chloroplast of Chlamydomonas are: codon optimization, protease activity, protein toxicity and transformation-associated genotypic modification.
authors.affiliation: PhycoBiologics Inc, Indianapolis IN, Bloomington, IN 47401, USA.
authors.forename: Raymond
authors.initials: R
authors.lastname: Surzycki
authors.forename: Katie
authors.initials: K
authors.lastname: Greenham
authors.forename: Kaoru
authors.initials: K
authors.lastname: Kitayama
authors.forename: Flora
authors.initials: F
authors.lastname: Dibal
authors.forename: Richard
authors.initials: R
authors.lastname: Wagner
authors.forename: Jean-David
authors.initials: JD
authors.lastname: Rochaix
authors.forename: Tarek
authors.initials: T
authors.lastname: Ajam
authors.forename: Stefan
authors.initials: S
authors.lastname: Surzycki
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D015153:Blotting, Western; D002696:Chlamydomonas / Q000235:genetics*; D003062:Codon; D005838:Genotype; D014614:Vaccines, Synthetic / Q000235:genetics*
chemical_list: D003062:Codon; D014614:Vaccines, Synthetic