Intramuscular delivery of a cholera DNA vaccine primes both systemic and mucosal protective antibody responses against cholera.
Cholera is a potentially lethal diarrhea disease caused by the gram-negative bacterium Vibrio cholerae. The need for an effective cholera vaccine is clearly indicated but the challenges of eliciting both systemic and mucosal immune responses remains a significant challenge. In the current report, we discovered that a DNA vaccine expressing a protective cholera antigen, cholera toxin B subunit (CTB), delivered parenterally can elicit both systemic and mucosal anti-CTB antibody responses in mice. The priming effect by DNA immunization was demonstrated by higher mucosal antibody responses following one boost with the inactivated cholera vaccine (KWC-B) delivered orally when compared to the twice oral administration of KWC-B alone. This finding indicates that DNA vaccines delivered parenterally are effective in eliciting mucosal protective immune responses--a unique advantage for DNA vaccination that has not yet been well realized and should bring value to the development of novel vaccination approaches against mucosally transmitted diseases.
Xu
Guifang
G
Department of Infectious Diseases, First Affiliated Hospital, Nanjing Medical University, Nanjing 210029, China.
Wang
Shixia
S
Zhuang
Ling
L
Hackett
Anthony
A
Gu
Ling
L
Zhang
Lu
L
Zhang
Chunhua
C
Wang
Hua
H
Huang
Zuhu
Z
Lu
Shan
S
D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis; D000992:Antitoxins / Q000032:analysis; D002771:Cholera / Q000276:immunology* / Q000517:prevention & control; D002772:Cholera Toxin / Q000008:administration & dosage* / Q000276:immunology*; D022121:Cholera Vaccines / Q000008:administration & dosage* / Q000276:immunology*; D018928:Immunity, Mucosal; D007273:Injections, Intramuscular; D051379:Mice; D008807:Mice, Inbred BALB C; D011817:Rabbits; D019444:Vaccines, DNA / Q000008:administration & dosage* / Q000276:immunology*
D000907:Antibodies, Bacterial; D000992:Antitoxins; D022121:Cholera Vaccines; D019444:Vaccines, DNA; D002772:Cholera Toxin