title: Recombination between linear double-stranded DNA substrates in vivo.
abstract: Recombineering technology in Escherichia coli enables targeting of linear donor DNA to circular recipient DNA using short shared homology sequences. In this work, we demonstrate that recombineering is also able to support recombination between a pair of linear DNA substrates (linear/linear recombineering) in vivo in E. coli. Linear DNA up to 100 kb is accurately modified and remains intact without undergoing rearrangements after recombination. This system will be valuable for direct in vivo manipulation of large linear DNA including the N15 and PY54 prophages and linear animal viruses, and for assembly of linear constructs as artificial chromosome vectors.
authors.affiliation: Department of Genetics and Genomic Sciences, Box 1498, Mount Sinai School of Medicine, 1425 Madison Avenue, EB 14-02, New York, NY 10029, USA. kumaran.narayanan@mssm.edu
authors.forename: Kumaran
authors.initials: K
authors.lastname: Narayanan
authors.forename: Edmund Ui-Hang
authors.initials: EU
authors.lastname: Sim
authors.forename: Nikolai V
authors.initials: NV
authors.lastname: Ravin
authors.forename: Choon-Weng
authors.initials: CW
authors.lastname: Lee
mesh_terms: D004247:DNA / Q000235:genetics*; D004269:DNA, Bacterial / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics; D005818:Genetic Engineering / Q000379:methods*; D011995:Recombination, Genetic
chemical_list: D004269:DNA, Bacterial; D004247:DNA