Human-urine diabetes assay and in vivo rat bladder assay using a fluorine-doped carbon nanotube catheter sensor.
The creation of a novel biosensor consisting of a fluorine-doped carbon nanotube (FCN) was explored for use in cyclic voltammetric (CV) and square-wave stripping voltammetric (SW) glucose assay. In the experiment that was carried out in this study, analytical optimum conditions were attained at the low detection limit (S/N3) of 0.6 microg/L (3.3 x 10(-9) M). In the 0.1 mg/L spike, the relative standard deviation of 0.607 (n = 15) was obtained. This was used for the diagnosis of the urine of patients with diabetes. Moreover, the catheter-type electrode (CE) can be inserted into a rat bladder through the rat's organs. Thus, it can be connected with an electrochemical analyzer that can be fitted with an interface for the real-time in vivo analysis of metabolic glucose. The developed system can be used for organ treatment, biological analysis, and in vivo control.
Ly
Suw Young
SY
Biosensor Research Institute, Seoul National University of Technology, Seoul, South Korea. suwyoung@snut.ac.kr
Lee
Jin Hui
JH
D000818:Animals; D015374:Biosensing Techniques; D002408:Catheters, Indwelling; D017076:Computer-Aided Design; D003920:Diabetes Mellitus / Q000175:diagnosis / Q000652:urine*; D004563:Electrochemistry / Q000295:instrumentation*; D004566:Electrodes; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005260:Female; D005461:Fluorine / Q000737:chemistry; D005947:Glucose; D006029:Glycosuria / Q000652:urine*; D008297:Male; D036103:Nanotechnology / Q000295:instrumentation; D037742:Nanotubes, Carbon / Q000737:chemistry*; D051381:Rats; D017208:Rats, Wistar; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D016482:Urinalysis / Q000295:instrumentation; D001743:Urinary Bladder / Q000378:metabolism*
D037742:Nanotubes, Carbon; D005461:Fluorine; D005947:Glucose