title: Recognition of Salmonella Typhimurium by Immobilized Phage P22 Monolayers.
abstract: Phages are promising alternatives to antibodies as the biorecognition element in a variety of biosensing applications. In this study, a monolayer of bacteriophage P22 whose tailspike proteins specifically recognize Salmonella serotypes was covalently bound to glass substrates through a bifunctional cross linker 3-aminopropyltrimethoxysilane. The specific binding of Salmonella typhimurium to the phage monolayer was studied by enzyme-linked immunosorbent assay and atomic force microscopy. Escherichia coli and a Gram-positive bacterium Listeria monocytogenes were also studied as control bacteria. The P22 particles show strong binding affinity to Salmonella typhimurium. In addition, the dried P22 monolayer maintained 50% binding capacity to Salmonella typhimurium after a one-week storage time. This is a promising method to prepare phage monolayer coatings on surface plasmon resonance and acoustic biosensor substrates in order to utilize the nascent phage display technology.
authors.affiliation: Department of Chemical Engineering and Materials Science, Wayne State University, Detroit, MI 48202, USA.
authors.forename: Hitesh
authors.initials: H
authors.lastname: Handa
authors.forename: Stephen
authors.initials: S
authors.lastname: Gurczynski
authors.forename: Matthew P
authors.initials: MP
authors.lastname: Jackson
authors.forename: Gregory
authors.initials: G
authors.lastname: Auner
authors.forename: Guangzhao
authors.initials: G
authors.lastname: Mao