Microarray analysis of gene expression in rat alveolar epithelial cells exposed to fractionated organic extracts of diesel exhaust particles.
Diesel exhaust particles (DEP) affect health adversely. Our previous studies revealed that DEP extracts up-regulated expression of genes related to drug metabolism, antioxidant enzymes, cell cycle/apoptosis and coagulation, and in addition, n-hexane soluble fraction (n-HSF) of DEP extracts contains aliphatic and polycyclic aromatic hydrocarbons, and n-hexane insoluble fraction (n-HISF) contains oxygenated compounds and has strong oxidative property. However, the relationship between the properties of chemicals in DEP extracts and the gene expression has not been fully elucidated. Here, we used a microarray analysis to identify and characterize genes whose expression is regulated by exposure to fractions of DEP extracts. A dichloromethane-soluble fraction (DMSF) of DEP was further fractionated into n-HSF and n-HISF. We exposed rat alveolar epithelial (SV40T2) cells to these fractions (30microg/ml) for 6h and identified regulated genes. DMSF predominantly up-regulated genes associated with drug metabolism (Cyp1a1, Gsta3), oxidative stress response (HO-1, Srxn1) and cell cycle/apoptosis (Okl38). Genes up-regulated by n-HSF were mainly associated with drug metabolism (Cyp1a1, Gsta3). The genes up-regulated by n-HISF included antioxidant enzymes (HO-1, Srxn1); genes response to cell damage, such as those functioning in cell cycle regulation or apoptosis (Okl38); and genes in coagulation pathways. Our present results suggested that n-HSF and n-HISF regulated characteristic genes which respond to chemical properties of each fraction.
Omura
Seiichi
S
National Institute for Environmental Studies, Onogawa, Tsukuba, Ibaraki, Japan.
Koike
Eiko
E
Kobayashi
Takahiro
T
D000818:Animals; D002460:Cell Line; D019363:Cytochrome P-450 CYP1A1 / Q000187:drug effects / Q000235:genetics; D004847:Epithelial Cells / Q000187:drug effects* / Q000378:metabolism; D005982:Glutathione Transferase / Q000187:drug effects / Q000235:genetics; D051547:Heme Oxygenase-1 / Q000187:drug effects / Q000235:genetics; D006586:Hexanes / Q000737:chemistry; D008752:Methylene Chloride / Q000737:chemistry; D020411:Oligonucleotide Array Sequence Analysis; D050862:Oxidoreductases Acting on Sulfur Group Donors / Q000187:drug effects / Q000235:genetics; D011506:Proteins / Q000187:drug effects / Q000235:genetics; D011650:Pulmonary Alveoli / Q000187:drug effects* / Q000378:metabolism; D051381:Rats; D015854:Up-Regulation / Q000187:drug effects*; D001335:Vehicle Emissions / Q000633:toxicity*
D006586:Hexanes; C492609:Osgin1 protein, rat; D011506:Proteins; D001335:Vehicle Emissions; C026385:n-hexane; D008752:Methylene Chloride; D019363:Cytochrome P-450 CYP1A1; D051547:Heme Oxygenase-1; D050862:Oxidoreductases Acting on Sulfur Group Donors; D005982:Glutathione Transferase