title: [Influence of Lactobacillus fermentum metabolites on ultrastructure of pathogenic Escherichia coli].
abstract: OBJECTIVE
  To study ultrastructural changes of hemolytic and enterohemorrhagic Escherichia coli during interaction with metabolites of Lactobacillus fermentum.
  
  
  METHODS
  Strains of pathogenic hemolytic (Hly) and enterohemorrhagic Escherichia coli (O157:H7) as well as symbiotic bacteriocinogenic strain Lactobacillus fermentum 97 were used. Inhibition of growth of viable cells was performed by delayed antagonism method. Using electron microscopy, assessment of ultrastructural changes of hemolytic and enterohemorrhagic E. coli under the influence of diffusing in MPC-agar metabolites of lactobacilli.
  
  
  RESULTS
  Changes pointing to profound destructive processes in bacterial cells were detected on ultrathin sections. Under the influence of diffusing metabolites of lactobacilli, the following changes were observed: destabilization of cell wall, expansion of periplasmatic space, and emergence of low electron density areas of cytoplasm in polar sections of cells with visualization of floccular material. Emergence of elongated paracrystallic packings and filamentous structures of different length, which deserve special study, was observed in cells of hemolytic E. coli.
  
  
  CONCLUSIONS
  Bacteriocin-like products of lactobacilli during interaction with pathogenic E. coli cause profound destructive changes in the latter which lead to destruction of target cells.
authors.forename: N A
authors.initials: NA
authors.lastname: Shabanova
authors.forename: V V
authors.initials: VV
authors.lastname: Gosteva
authors.forename: N V
authors.initials: NV
authors.lastname: Klitsunova
authors.forename: V M
authors.initials: VM
authors.lastname: Bondarenko
mesh_terms: D000898:Antibiosis; D001430:Bacteriocins / Q000378:metabolism; D019453:Escherichia coli O157 / Q000472:pathogenicity / Q000648:ultrastructure*; D006459:Hemolysin Factors / Q000378:metabolism; D052197:Limosilactobacillus fermentum / Q000254:growth & development / Q000378:metabolism*; D008854:Microscopy, Electron
chemical_list: D001430:Bacteriocins