Site-specific covalent attachment of DNA to proteins using a photoactivatable Tus-Ter complex.
Investigations into the photocrosslinking kinetics of the protein Tus with various bromodeoxyuridine-substituted Ter DNA variants highlight the potential use of this complex as a photoactivatable connector between proteins of interest and specific DNA sequences.
Dahdah
Dahdah B
DB
Comparative Genomics Centre, School of Pharmacy & Molecular Sciences, James Cook University, Townsville, QLD 4811, Australia.
Morin
Isabelle
I
Moreau
Morgane J J
MJ
Dixon
Nicholas E
NE
Schaeffer
Patrick M
PM
D004247:DNA / Q000737:chemistry*; D004268:DNA-Binding Proteins / Q000737:chemistry*; D029968:Escherichia coli Proteins / Q000737:chemistry*; D007700:Kinetics; D010777:Photochemistry; D011506:Proteins / Q000737:chemistry*
C062011:DNA replication terminus site-binding protein, E coli; D004268:DNA-Binding Proteins; D029968:Escherichia coli Proteins; D011506:Proteins; C489236:tus protein, E coli; D004247:DNA