Cytotoxicity evaluation of Activ GP and Resilon sealers in vitro.
OBJECTIVE
This study is to evaluate the cytotoxicity of Activ GP and RealSeal sealers in a cell culture system in vitro, and to compare them with traditional AH 26 and Kerr sealers.


METHODS
Samples of 0.5 mg freshly mixed or set RealSeal, Activ GP, AH 26, and Kerr sealers were eluted with 200, 400, 800, and 1,200 microL cell culture medium for 24 and 72 hours. L929 cells were seeded into 96-well plates at 3 x 10(4) cells/well and cultured with 100 microL eluate from each eluate group. Cells cultured with culture medium only served as a control. After 24 hours' incubation the cytotoxicity was evaluated by MTT assay. Cell viability was calculated as the percentage of the control group, and the results were analyzed with 1-way analysis of variance.


RESULTS
For the freshly mixed sealer, cell viability in the AH 26 group was less than in all of the other 3 sealer groups. The Kerr sealer group had greater cell viability than RealSeal and Activ GP groups. For the set sealer, cell viability in the AH 26 group was greater than in all of the other 3 groups. Cell viability in the RealSeal group was less than in the Kerr and Activ GP groups.


CONCLUSIONS
Freshly mixed RealSeal and Activ GP sealers have lower cytotoxicity than AH 26 sealer and more cytotoxicity than Kerr sealer. When sealers are set, RealSeal sealer has more cytotoxicity than AH 26 and Kerr sealer. Activ GP sealer has more cytotoxicity than AH 26 and is similar to Kerr sealer.
Donadio
Marco
M
Division of Endodontology, School of Dental Medicine, The University of Connecticut Health Center, Farmington, Connecticut 06030-1715, USA.
Jiang
Jin
J
He
Jianing
J
Wang
Yu-Hsiung
YH
Safavi
Kamran E
KE
Zhu
Qiang
Q
D000180:Acrylic Resins / Q000633:toxicity*; D000818:Animals; D002470:Cell Survival; D001840:Dental Bonding / Q000379:methods; D004305:Dose-Response Relationship, Drug; D005899:Glass Ionomer Cements / Q000633:toxicity; D007739:L Cells; D051379:Mice; D012387:Root Canal Filling Materials / Q000633:toxicity*; D015035:Zinc Oxide-Eugenol Cement / Q000633:toxicity
D000180:Acrylic Resins; D005899:Glass Ionomer Cements; D012387:Root Canal Filling Materials; D015035:Zinc Oxide-Eugenol Cement; C528807:activ GP sealer; C486892:resilon sealer; C076543:Kerr Pulp Canal Sealer