One-pot fermentation of pladienolide D by Streptomyces platensis expressing a heterologous cytochrome P450 gene.
Pladienolide D is a 16-hydroxylated derivative of pladienolide B, produced by Streptomyces platensis. To facilitate the production of pladienolide D, the gene encoding a pladienolide B 16-hydroxylase from S. bungoensis was introduced into S. platensis. The recombinant produced pladienolide D at a production level comparable to that of pladienolide B.
Machida
Kazuhiro
K
Bioresource Laboratories, Mercian Corp., 1808 Nakaizumi, Iwata, Shizuoka 438-0078, Japan. machida-k@mercian.co.jp
Aritoku
Yasuhide
Y
Tsuchida
Toshio
T
D001426:Bacterial Proteins / Q000378:metabolism; D003001:Cloning, Molecular; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism*; D004852:Epoxy Compounds / Q000378:metabolism*; D005285:Fermentation; D018942:Macrolides / Q000378:metabolism*; D013302:Streptomyces / Q000378:metabolism*
D001426:Bacterial Proteins; D004852:Epoxy Compounds; D018942:Macrolides; C522342:pladienolide B; C522343:pladienolide D; D003577:Cytochrome P-450 Enzyme System