High performance liquid chromatography determination of sulphachloropyrazine residues in broiler and turkey edible tissues.
A HPLC method to determine and quantify sulphachloropyrazine residues from broilers and turkeys is reported. This procedure permitted sulphachloropyrazine to be separated from muscle tissue, liver, kidneys and fat with skin after extraction with dichloromethane under slightly acidic conditions. The analytical methodology showed a high specificity and sensitivity and an adequate precision and accuracy with a limit of quantification of 56 ng mL(-1). The peak area showed a linear relationship with a concentration over the range 50-750 ng mL(-1) for sulphachloropyrazine standard solutions. Recovery dates were also satisfactory with values between 69.7 and 77.5%.
Kowalski
C J
CJ
Department of Pharmacology, Faculty of Veterinary Medicine, University of Life Science, Lublin, Poland. cezary.kowalski@up.lublin.pl
Łebkowska-Wieruszewska
B
B
Osypiuk
M
M
D000818:Animals; D000890:Anti-Infective Agents / Q000032:analysis*; D002645:Chickens; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005506:Food Contamination; D012015:Reference Standards; D012680:Sensitivity and Specificity; D013424:Sulfanilamides / Q000032:analysis*; D014018:Tissue Distribution; D014422:Turkeys; D000096926:Benzenesulfonamides
D000890:Anti-Infective Agents; D013424:Sulfanilamides; C050643:sulfachlorpyrazine; D000096926:Benzenesulfonamides