Efficient replication bypass of size-expanded DNA base pairs in bacterial cells.
Supersize me! Size-expanded DNA bases (xDNA) are able to encode natural DNA sequences in replication. In vitro experiments with a DNA polymerase show nucleotide incorporation opposite the xDNA bases with correct pairing. In vivo experiments using E. coli show that two xDNA bases (xA and xC, see picture) encode the correct replication partners.
Delaney
James C
JC
Department of Chemistry, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
Gao
Jianmin
J
Liu
Haibo
H
Shrivastav
Nidhi
N
Essigmann
John M
JM
Kool
Eric T
ET
D020029:Base Pairing; D004247:DNA / Q000737:chemistry*; D004261:DNA Replication; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D007700:Kinetics; D009711:Nucleotides / Q000737:chemistry*
D009711:Nucleotides; D004247:DNA; D004259:DNA-Directed DNA Polymerase