FluidFM: combining atomic force microscopy and nanofluidics in a universal liquid delivery system for single cell applications and beyond.
We describe the fluidFM, an atomic force microscope (AFM) based on hollow cantilevers for local liquid dispensing and stimulation of single living cells under physiological conditions. A nanofluidic channel in the cantilever allows soluble molecules to be dispensed through a submicrometer aperture in the AFM tip. The sensitive AFM force feedback allows controlled approach of the tip to a sample for extremely local modification of surfaces in liquid environments. It also allows reliable discrimination between gentle contact with a cell membrane or its perforation. Using these two procedures, dyes have been introduced into individual living cells and even selected subcellular structures of these cells. The universality and versatility of the fluidFM will stimulate original experiments at the submicrometer scale not only in biology but also in physics, chemistry, and material science.
Meister
André
A
Swiss Center for Electronics and Microtechnology, CSEM SA, rue Jaquet Droz 1, 2002 Neuchatel, Switzerland.
Gabi
Michael
M
Behr
Pascal
P
Studer
Philipp
P
Vörös
János
J
Niedermann
Philippe
P
Bitterli
Joanna
J
Polesel-Maris
Jérôme
J
Liley
Martha
M
Heinzelmann
Harry
H
Zambelli
Tomaso
T
D000818:Animals; D045744:Cell Line, Tumor; D002462:Cell Membrane / Q000502:physiology* / Q000648:ultrastructure; D051379:Mice; D008845:Microinjections / Q000379:methods*; D018625:Microscopy, Atomic Force / Q000379:methods*; D036103:Nanotechnology / Q000379:methods*; D051381:Rats