title: Isolation and immunomodulatory effect of homoisoflavones and flavones from Agave sisalana Perrine ex Engelm.
abstract: Three known flavones and seven known homoisoflavonoids were isolated from the methanolic extract of the leaves of Agave sisalanaPerrine ex Engelm. Their structures were elucidated on the basis of spectroscopic analysis. The isolated compounds were also evaluated for immunopharmacological activity. PBMC were used as target cells, and cell proliferation was determined by (3)H-thymidine uptake. (+/-)-3,9-Dihydroeucomin (4), dihydrobonducellin (5), and 5,7-dihydroxy-3-(4'-hydroxybenzyl)-4-chromanone (7) showed inhibitory effects on PBMC proliferation activated by PHA with IC(50) values 19.4, 73.8, and 58.8 microM, respectively. All three compounds significantly inhibited the production of IL-2 and IFN-gamma in activated PBMC in a concentration-dependent manner.
authors.affiliation: Graduate Institute of Pharmacy, Taipei Medical University, Taipei, Taiwan.e023089103@tmu.edu.tw
authors.forename: Pi Yu
authors.initials: PY
authors.lastname: Chen
authors.forename: Yuh Chi
authors.initials: YC
authors.lastname: Kuo
authors.forename: Chin Hui
authors.initials: CH
authors.lastname: Chen
authors.forename: Yueh Hsiung
authors.initials: YH
authors.lastname: Kuo
authors.forename: Ching Kuo
authors.initials: CK
authors.lastname: Lee
mesh_terms: D027882:Agave / Q000033:anatomy & histology / Q000737:chemistry* / Q000276:immunology; D047309:Flavones / Q000276:immunology / Q000302:isolation & purification; D007155:Immunologic Factors / Q000276:immunology / Q000302:isolation & purification; D007529:Isoflavones / Q000276:immunology / Q000302:isolation & purification; D015394:Molecular Structure; D010936:Plant Extracts / Q000276:immunology / Q000302:isolation & purification; D018515:Plant Leaves / Q000737:chemistry
chemical_list: D047309:Flavones; D007155:Immunologic Factors; D007529:Isoflavones; D010936:Plant Extracts