title: Lamina-specific changes in hippocampal GABA(A)/cBZR and mossy fibre sprouting during and following amygdala kindling in the rat.
abstract: Alterations in hippocampal GABA(A)/central benzodiazepine receptor (GABA(A)/cBZR) expression and mossy fibre sprouting (MFS) may have aetiological significance in temporal lobe epileptogenesis. Their relationship with each other is also unknown. We utilised [3H]-flumazenil autoradiography to quantify changes in GABA(A)/cBZR density and affinity in all hippocampal laminae, and Timm's staining for MFS at different stages of epileptogenesis in the amygdala kindling rat model (after 24 stimulations, 48 stimulations and two weeks post-kindling). During kindling, receptor density was significantly elevated within the dentate stratum moleculare and granulosum, but decreased within the stratum radiatum of CA3 and CA2. Two weeks post-kindling, receptor density remained upregulated in the dentate stratum moleculare and was also upregulated in CA3 stratum oriens and CA1 stratum moleculare. MFS was significantly increased in the dentate stratum moleculare at two weeks post-kindling, with a strong inverse correlation between MFS and GABA(A)/cBZR density in this region. No changes in GABA(A)/cBZR binding affinity were detected for any hippocampal subregion at any time point. Our results demonstrate that changes in hippocampal GABA(A)/cBZR expression are lamina- and time-specific. Within the dentate gyrus, receptor density is upregulated throughout epileptogenesis, whilst within the hippocampus proper, receptor density is downregulated early in epileptogenesis but upregulated at the chronic phase. A novel association between MFS and GABA(A)/cBZR density has been demonstrated by this study, which could represent an important compensatory or pathological mechanism associated with epileptogenesis.
authors.affiliation: The Department of Medicine, The Royal Melbourne Hospital, The University of Melbourne, 4th Floor Clinical Sciences Building, Royal Parade, Parkville 3050, Australia.
authors.forename: David S H
authors.initials: DS
authors.lastname: Liu
authors.forename: Terence J
authors.initials: TJ
authors.lastname: O'Brien
authors.forename: David A
authors.initials: DA
authors.lastname: Williams
authors.forename: Rodney J
authors.initials: RJ
authors.lastname: Hicks
authors.forename: Damian E
authors.initials: DE
authors.lastname: Myers
mesh_terms: D000679:Amygdala / Q000503:physiopathology*; D000818:Animals; D001345:Autoradiography; D002452:Cell Count; D016923:Cell Death; D004558:Electric Stimulation; D004567:Electrodes, Implanted; D004827:Epilepsy / Q000503:physiopathology*; D005442:Flumazenil; D006624:Hippocampus / Q000503:physiopathology*; D007696:Kindling, Neurologic; D008297:Male; D009474:Neurons / Q000502:physiology*; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D011963:Receptors, GABA-A / Q000378:metabolism*; D012640:Seizures / Q000503:physiopathology; D013997:Time Factors; D014316:Tritium
chemical_list: D011963:Receptors, GABA-A; D014316:Tritium; D005442:Flumazenil