[Genotyping of AKAP10 gene 2073A/G single nucleotide polymorphism by TaqMan probe real-time PCR].
OBJECTIVE
To detect AKAP10 gene 2073A/G single nucleotide polymorphism (SNP) genotyping by TaqMan probe real-time PCR.


METHODS
The genotype of AKAP10 gene 2073A/G was detected by real-time PCR with a pair of new-designed TaqMan probes. The PCR products also were subjected to gene sequence analysis to validate the results of TaqMan probe real-time PCR.


RESULTS
The TaqMan probe real-time PCR method was successfully developed to detect AKAP10 gene 2073A/G SNP. The results were accordant with those achieved by DNA sequencing. The distribution of AKAP10 gene 2073A/G among population had no relationship with gender, age (P > 0.05). Unconditional logistic regression analysis revealed that the variant genotypes (AG + GG) had a 52% increased risk of colorectal cancer, compared with the AA genotype (P = 0.019).


CONCLUSIONS
A detection platform for SNP genotyping by TaqMan probe was set up successfully. There was a significant association between AKAP10 gene 2073A/G and colorectal cancer.
Wang
Mo-jin
MJ
Institute of Digestive Surgery, West China Hospital, Sichuan University, Chengdu 610041, China.
Zhou
Zong-guang
ZG
Wang
Ling
L
Li
Yuan
Y
Zhang
Peng
P
Zhang
Yi
Y
Cui
Chang-fu
CF
Zhou
Bin
B
D054758:A Kinase Anchor Proteins / Q000235:genetics*; D015179:Colorectal Neoplasms / Q000209:etiology / Q000235:genetics*; D015342:DNA Probes / Q000235:genetics; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods; D020641:Polymorphism, Single Nucleotide; D017422:Sequence Analysis, DNA
D054758:A Kinase Anchor Proteins; C484639:AKAP10 protein, human; D015342:DNA Probes