title: Spontaneous changes in tumour cell dissemination to bone marrow in colorectal cancer.
abstract: OBJECTIVE
  The study aimed to evaluate the incidence of disseminated tumour cells (DTCs) in bone marrow (BM) preoperatively and during follow up and to correlate these with established risk factors in patients with colorectal cancer.
  
  
  METHODS
  We prospectively studied BM in 57 patients using the anti-cytokeratin antibody A45-B/B3.
  
  
  RESULTS
  The overall detection rate of DTCs was 23% with a similar detection rate through all stages of the disease. No significant association was found between the presence of DTCs and clinicopathological parameters. After a median follow up of 35.4 months, no differences were found in relapse and overall survival between patients with and without DTC preoperatively. In 31 of 45 patients with local disease, we performed a follow-up BM examination after 1 year. In 26% of the patients, the BM status had changed as compared with the preoperative finding.
  
  
  CONCLUSIONS
  This is the first study to report the follow up of DTC in BM in colorectal cancer using the A45-B/B3 antibody. The presence of tumour cells in the preoperative BM had no impact on outcome. The BM status had changed after 12 months in a quarter of patients.
authors.affiliation: 2nd Department of Medicine, Ludwig Boltzmann Society, Donauspital, Langobardenstrasse 122, 1220 Vienna. veronika.buxhofer@wienkav.at
authors.forename: V
authors.initials: V
authors.lastname: Buxhofer-Ausch
authors.forename: C
authors.initials: C
authors.lastname: Ausch
authors.forename: E
authors.initials: E
authors.lastname: Kitzweger
authors.forename: M
authors.initials: M
authors.lastname: Mollik
authors.forename: A
authors.initials: A
authors.lastname: Reiner-Concin
authors.forename: E
authors.initials: E
authors.lastname: Ogris
authors.forename: M
authors.initials: M
authors.lastname: Stampfl
authors.forename: G
authors.initials: G
authors.lastname: Hamilton
authors.forename: R
authors.initials: R
authors.lastname: Schiessel
authors.forename: W
authors.initials: W
authors.lastname: Hinterberger
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D000911:Antibodies, Monoclonal; D001853:Bone Marrow / Q000737:chemistry / Q000473:pathology*; D015179:Colorectal Neoplasms / Q000473:pathology* / Q000601:surgery; D018450:Disease Progression; D018572:Disease-Free Survival; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D007633:Keratins / Q000032:analysis; D008297:Male; D008875:Middle Aged; D009362:Neoplasm Metastasis; D011379:Prognosis; D011446:Prospective Studies; D018709:Statistics, Nonparametric; D013997:Time Factors
chemical_list: D000911:Antibodies, Monoclonal; D007633:Keratins