title: pfaB products determine the molecular species produced in bacterial polyunsaturated fatty acid biosynthesis.
abstract: When pDHA4, a vector carrying all five pfaA-pfaE genes responsible for docosahexaenoic acid (DHA; 22:6) biosynthesis in Moritella marina MP-1, was coexpressed in Escherichia coli with the individual pfaA-pfaD genes for eicosapentaenoic acid (EPA; 20:5) biosynthesis from Shewanella pneumatophori SCRC-2738, both polyunsaturated fatty acids were synthesized only in the recombinant carrying pfaB for EPA synthesis. Escherichia coli coexpressing a deleted construct comprising pfaA, pfaC, pfaD and pfaE for EPA and pfaB for DHA produced EPA and DHA. Both EPA and DHA were detected in bacteria that inherently contained pfa genes for DHA. These results suggest that PfaB is the key enzyme determining the final product in EPA or DHA biosynthesis.
authors.affiliation: Laboratory of Environmental Molecular Biology, Faculty of Environmental Earth Science, Hokkaido University, Sapporo, Japan.
authors.forename: Yoshitake
authors.initials: Y
authors.lastname: Orikasa
authors.forename: Mika
authors.initials: M
authors.lastname: Tanaka
authors.forename: Shinji
authors.initials: S
authors.lastname: Sugihara
authors.forename: Ryuji
authors.initials: R
authors.lastname: Hori
authors.forename: Takanori
authors.initials: T
authors.lastname: Nishida
authors.forename: Akio
authors.initials: A
authors.lastname: Ueno
authors.forename: Naoki
authors.initials: N
authors.lastname: Morita
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Yano
authors.forename: Kouhei
authors.initials: K
authors.lastname: Yamamoto
authors.forename: Akira
authors.initials: A
authors.lastname: Shibahara
authors.forename: Hidenori
authors.initials: H
authors.lastname: Hayashi
authors.forename: Yohko
authors.initials: Y
authors.lastname: Yamada
authors.forename: Akiko
authors.initials: A
authors.lastname: Yamada
authors.forename: Reiko
authors.initials: R
authors.lastname: Yu
authors.forename: Kazuo
authors.initials: K
authors.lastname: Watanabe
authors.forename: Hidetoshi
authors.initials: H
authors.lastname: Okuyama
mesh_terms: D001426:Bacterial Proteins / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D004281:Docosahexaenoic Acids / Q000737:chemistry* / Q000378:metabolism; D015118:Eicosapentaenoic Acid / Q000096:biosynthesis / Q000737:chemistry*; D005231:Fatty Acids, Unsaturated / Q000096:biosynthesis* / Q000737:chemistry; D005786:Gene Expression Regulation; D015964:Gene Expression Regulation, Bacterial; D034221:Moritella / Q000235:genetics / Q000378:metabolism*; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D005231:Fatty Acids, Unsaturated; D011994:Recombinant Proteins; D004281:Docosahexaenoic Acids; D015118:Eicosapentaenoic Acid