title: The effect of menaquinone-7 (vitamin K2) supplementation on osteocalcin carboxylation in healthy prepubertal children.
abstract: Vitamin K contributes to bone health, probably through its role as cofactor in the carboxylation of osteocalcin. Intervention studies in adults have demonstrated that markedly higher osteocalcin carboxylation is obtained by intakes of vitamin K well above the current recommended dietary intake. However, the relationship between increased vitamin K2 intake and enhanced osteocalcin carboxylation has never been shown in healthy children. The objective was to study the effect of 45 microg menaquinone-7 (MK-7; one of the vitamin K2 species) on the circulating levels of undercarboxylated osteocalcin (ucOC) and carboxylated osteocalcin (cOC) in healthy prepubertal children. We hypothesised that MK-7 supplementation will reduce the ucOC:cOC ratio (UCR), indicating an improved vitamin K status. The present study is a double-blind randomised placebo-controlled trial examining the effect of 8 weeks MK-7 supplementation on the carboxylation of osteocalcin in healthy children (n 55). Serum levels of ucOC, cOC and MK-7 were measured at baseline and after 8 weeks, together with bone markers and coagulation parameters. The UCR was used as an indicator of vitamin K status. In the MK-7-supplemented group (n 28), the circulating concentration of inactive ucOC reduced and the UCR improved whereas the concentration of MK-7 increased. Within the placebo group, ucOC, cOC, UCR and MK-7 did not significantly change over time. In both groups, bone markers and coagulation parameters remained constant over time. These findings demonstrate that in healthy, prepubertal children, modest supplementation with MK-7 increases circulating concentrations of MK-7 and increases osteocalcin carboxylation.
authors.affiliation: Department of Paediatrics, University Medical Centre Utrecht, PO Box 85090, 3508 AB Utrecht, The Netherlands. m.j.h.vansummeren@umcutrecht.nl
authors.forename: Marieke J H
authors.initials: MJ
authors.lastname: van Summeren
authors.forename: Lavienja A J L M
authors.initials: LA
authors.lastname: Braam
authors.forename: Marc R
authors.initials: MR
authors.lastname: Lilien
authors.forename: Leon J
authors.initials: LJ
authors.lastname: Schurgers
authors.forename: Wietse
authors.initials: W
authors.lastname: Kuis
authors.forename: Cees
authors.initials: C
authors.lastname: Vermeer
mesh_terms: D000886:Anthropometry; D015415:Biomarkers / Q000097:blood; D001777:Blood Coagulation / Q000187:drug effects; D016723:Bone Remodeling / Q000187:drug effects / Q000502:physiology; D002264:Carboxylic Acids / Q000097:blood; D002648:Child; D002664:Child Nutritional Physiological Phenomena; D019587:Dietary Supplements; D004311:Double-Blind Method; D005260:Female; D006801:Humans; D008297:Male; D015675:Osteocalcin / Q000097:blood*; D024482:Vitamin K 2 / Q000031:analogs & derivatives* / Q000097:blood / Q000494:pharmacology; D014815:Vitamins / Q000097:blood / Q000494:pharmacology*
chemical_list: D015415:Biomarkers; D002264:Carboxylic Acids; D014815:Vitamins; D015675:Osteocalcin; D024482:Vitamin K 2; C062629:menaquinone 7