Isolation and characterization of NgRLK1, a receptor-like kinase of Nicotiana glutinosa that interacts with the elicitin of Phytophthora capsici.
Elicitins, extracellular proteins from Phytophthora fungi, elicit a hypersensitivity response (HR), including systemic acquired resistance, in some plants. The elicitin capsicein (approximately 10 kDa) was purified by FPLC from culture filtrates of P. capsici. Purified native and recombinant capsicein induced a hypersensitive response in leaves of the non-host plants Nicotiana glutinosa and Brassica rapa subsp. pekinensis. To search for candidate capsicein-interacting proteins from N. glutinosa, a yeast two-hybrid assay was used. We identified a protein interactor that is homologous to a serine/threonine kinase of the plant receptor-like kinase (RLK) group and designated it NgRLK1. The ORF of NgRLK1 encodes a polypeptide of 832 amino acids (93,490 Da). A conserved domain analysis revealed that NgRLK1 has structural features typical of a plant RLK. NgRLK1 was autophosphorylated, with higher activity in the presence of Mn2+ than Mg2+.
Kim
Yeong-Tae
YT
National Institute of Agricultural Science, Rural Development Administration, Suwon, 441-707, Korea. ytkim33@rda.go.kr
Oh
Jonghee
J
Kim
Kyung-Hwan
KH
Uhm
Jae-Youl
JY
Lee
Byoung-Moo
BM
D000595:Amino Acid Sequence; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism; D001483:Base Sequence; D029741:Brassica rapa / Q000276:immunology / Q000378:metabolism; D003001:Cloning, Molecular; D005656:Fungal Proteins / Q000302:isolation & purification / Q000378:metabolism*; D007113:Immunity, Innate / Q000235:genetics / Q000502:physiology; D008969:Molecular Sequence Data; D010802:Phylogeny; D010838:Phytophthora / Q000378:metabolism; D010935:Plant Diseases / Q000235:genetics; D018515:Plant Leaves / Q000737:chemistry / Q000276:immunology / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D011485:Protein Binding; D011494:Protein Kinases / Q000235:genetics* / Q000378:metabolism*; D017385:Sequence Homology; D014026:Nicotiana / Q000201:enzymology / Q000235:genetics* / Q000276:immunology / Q000378:metabolism
D029681:Arabidopsis Proteins; D005656:Fungal Proteins; D010940:Plant Proteins; C061033:Alpha-elicitin capsicein protein, Phytophthora capsici; D011494:Protein Kinases; C105762:RPK1 protein, Arabidopsis