Bacillus tianmuensis sp. nov., isolated from soil in Tianmu Mountain national natural reserve, Hangzhou, China.
In a project aiming to isolate strains with the ability to produce nonribosomal peptides, a gram-negative, endospore-forming, rod-shaped strain, designated B5(T), was isolated from a soil sample collected from Tianmu Mountain national natural reserve in Hangzhou, China. Strain B5(T) contained meso-diaminopimelic acid in the cell wall peptidoglycan. The major cellular fatty acids were anteiso-C(15:0) and iso-C(15:0). The DNA G+C content was 42.5 mol%. The phylogenetic analysis based on 16S rRNA gene sequence indicated that strain B5(T) fell within the genus Bacillus, with highest sequence similarity values to Bacillus barbaricus DSM 14730(T) (96.4%) and Bacillus macauensis JCM 13285(T) (95.5%). The isolate, however, could be distinguished from Bacillus strains with validly published names by low 16S rRNA gene sequence similarity values, distinct phenotypic and chemotaxonomic characteristics. On the basis of these polyphasic evidences, it is demonstrated that the isolate B5(T) represents a novel species of the genus Bacillus, for which the name Bacillus tianmuensis sp. nov. is proposed. The type strain is B5(T) (=DSM 22111(T)=CGMCC 1.8879(T)).
Wen
Yan-Ping
YP
Institute of Microbiology, College of Life Sciences, Zhejiang University, Hangzhou, China.
Wu
Xue-Chang
XC
Qian
Chao-Dong
CD
Zhao
Yu-Hua
YH
Fang
Hai-Huan
HH
Li
Ou
O
D001407:Bacillus / Q000737:chemistry / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D015373:Bacterial Typing Techniques; D001482:Base Composition; D002473:Cell Wall / Q000737:chemistry; D002681:China; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D003960:Diaminopimelic Acid / Q000032:analysis; D005227:Fatty Acids / Q000032:analysis; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D003960:Diaminopimelic Acid