Treatment of injured neurons with bone marrow stem cells cotransfected by hTERT and Ad-BDNF in vitro.
The purpose of this paper is to study the anti-injury effect of bone marrow stromal cells (BMSCs) transfected by human telomerase reverse transcriptase (hTERT) and adenovirous brain-derived neurotrophic factor (Ad-BDNF) in injured neurons. Rat injured neurons were induced in vitro and then cocultured with rBMSCs transfected by hTERT and Ad-BDNF. The anti-injury effect of rBMSCs transfected by hTERT and Ad-BDNF was detected by Western blot for neural apoptosis gene MAP2 and NF-kappaB and lactic dehydrogenase released by injured neurons. Our data demonstrated that graft rBMSCs transfected by hTERT and Ad-BDNF could increase the expression of BDNF in injured neurons and alleviate apoptosis of the injured neurons. According to these data, BDNF-BMSCs demonstrate a tendency to be protection against neuronal death caused by apoptosis which would last a long time after neural injury. The data also show the potential of BDNF-BMSCs as a promising therapeutic strategy that warrants further investigation in patients with traumatic brain injury.
Wang
Zhitao
Z
Department of Neurosurgery, Tianjin Medical University General Hospital, Tianjin Neurologic Institute, Tianjin, 300052, Peoples's Republic of China.
Deng
Quanjun
Q
Zhang
Xiaohui
X
Zhang
Jianning
J
D000256:Adenoviridae / Q000235:genetics / Q000378:metabolism; D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology / Q000502:physiology*; D016026:Bone Marrow Transplantation; D019208:Brain-Derived Neurotrophic Factor / Q000235:genetics / Q000378:metabolism*; D048430:Cell Shape; D002478:Cells, Cultured; D018920:Coculture Techniques; D006801:Humans; D009416:Nerve Regeneration / Q000502:physiology; D009474:Neurons / Q000473:pathology / Q000502:physiology; D051381:Rats; D017207:Rats, Sprague-Dawley; D013234:Stem Cells / Q000166:cytology / Q000502:physiology*; D019098:Telomerase / Q000235:genetics / Q000378:metabolism*
D019208:Brain-Derived Neurotrophic Factor; C509186:TERT protein, human; D019098:Telomerase