[Effects of 18beta-glycyrrhizic acid and 18alpha-glycyrrhizic acid on mRNA and protein expression of cytochrome P450 3A in cultured rat primary hepatocyte].
OBJECTIVE
To study the effects of 18beta-glycyrrhizic acid and 18alpha-glycyrrhizic acid on mRNA and protein expression of cytochrome P450 3A in cultured rat primary hepatocyte.


METHODS
Rat heaptocyte were isolated by two-step in situ collagenase perfusion method; the hepatocytes were seeded in dishes coated with type I rat tail collagen, culture medium was added and changed daily after gelation, the effect of 18beta-glycyrrhizic acid and 18alpha-glycyrrhizic acid on the expression of CYP3A was determined by reverse transcriptase-polymerase chain reaction (RT-PCR) and western blot respectively.


RESULTS
Treatment with 18beta-glycyrrhizic acid of primary rat hepatocytes resulted in marked up-regulation of CYP3A1 expression at both mRNA and protein levels in a concentration dependent manner, while exposure to 18alpha-glycyrrhizic acid of primary rat hepatocytes resulted in marked down-regulation of CYP3A1 expression at both mRNA and protein levels in a concentration dependent manmer.


CONCLUSIONS
18beta-glycyrrhizic acid and 18alpha-glycyrrhizic acid up-regulate and down-regulate CYP3A1 expression at the transcriptive levels.
Wang
Yuguang
Y
Beijing Institute of Radiation Medicine, Beijing 100850, China.
Yang
Minghui
M
Ma
Zengchun
Z
Liang
Qiande
Q
Tan
Hongling
H
Xiao
Chengrong
C
Gao
Yue
Y
D000818:Animals; D019411:Clinical Laboratory Techniques; D051544:Cytochrome P-450 CYP3A / Q000235:genetics / Q000378:metabolism*; D019695:Glycyrrhizic Acid / Q000494:pharmacology*; D022781:Hepatocytes / Q000187:drug effects* / Q000378:metabolism; D014176:Protein Biosynthesis / Q000187:drug effects*; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D017208:Rats, Wistar; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D014158:Transcription, Genetic / Q000187:drug effects* / Q000235:genetics
D012333:RNA, Messenger; D019695:Glycyrrhizic Acid; D051544:Cytochrome P-450 CYP3A