title: Fusion of images in multistep analysis of neovascularisation.
abstract: OBJECTIVE
  The aim of our study was to develop a method for the fusion of images received after repeated staining of the same sample taking into account spatial differences between the images.
  
  
  METHODS
  A method of objective fusion performance was investigated on the images receiving during multistep staining of the xenograft tumour cross-sections.
  
  
  RESULTS
  It was shown that several images receiving from different steps of staining procedures may be successfully fused by fluorescent marking of slide position with Trout red blood cells before analysis.
  
  
  CONCLUSIONS
  Proposed technique provides an accurate rigid fusion of light and fluorescent images receiving during multistep image analysis under microscope and may be applied for study of neovascularisation.
authors.affiliation: OncoRay (Center for Radiation Research in Oncology), University of Technology-Dresden, Dresden, Germany. sergey.tokalov@oncoray.de
authors.forename: Sergey V
authors.initials: SV
authors.lastname: Tokalov
authors.forename: Andrij M
authors.initials: AM
authors.lastname: Abramyuk
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D006801:Humans; D008297:Male; D008856:Microscopy, Fluorescence; D009368:Neoplasm Transplantation; D009369:Neoplasms / Q000098:blood supply* / Q000473:pathology*; D009389:Neovascularization, Pathologic / Q000473:pathology*; D051381:Rats; D011923:Rats, Nude; D013194:Staining and Labeling / Q000379:methods*