title: Antibacterial activity of ciprofloxacin and trimethoprim, alone and in combinittion, against Vibrio cholerae O1 biotype El Tor serotype Ogawa isolates.
abstract: In this communication, the ciprofloxacin-trimethoprim (Cp-Tm) combination showed synergistic (Fractional Inhibitory Concentration, FIC index 0.399) and additive (FIC index 0.665-0.83) effects against Vibrio cholerae O1 biotype El Tor serotype Ogawa isolates having Cp MICs 10 microg/ml and Cp 0.66 microg/ml, respectively, following agar dilution checkerboard method. The time-kill study results demonstrated synergy between Cp and Tm against both groups of isolates providing 2.04 log10 (for strain with Cp MIC 0.66 microg/ml) and 3.12 log10 (for strain with Cp MIC 10 microg/ml) decreases in CFU/ml between the combination and its most active compound. Thus, the findings of the present study suggest an introduction of Cp-Tm combination treatment regimen against drug resistant cholera and this in turn will help in combating the drug resistance of V. cholerae O1 biotype El Tor serotype Ogawa.
authors.affiliation: Department of Microbiology, Bacteriology and Serology Unit, Calcutta School of Tropical Medicine, C. R. Avenue, Kolkata-700 073, India. samtropmed@gmail.com
authors.forename: Shyamapada
authors.initials: S
authors.lastname: Mandal
authors.forename: Nishith K
authors.initials: NK
authors.lastname: Pal
authors.forename: Imran H
authors.initials: IH
authors.lastname: Chowdhury
authors.forename: Manisha
authors.initials: M
authors.lastname: Debmandal
mesh_terms: D000892:Anti-Infective Agents, Urinary / Q000627:therapeutic use; D002771:Cholera / Q000188:drug therapy*; D002939:Ciprofloxacin / Q000627:therapeutic use*; D024901:Drug Resistance, Multiple, Bacterial; D004359:Drug Therapy, Combination; D006801:Humans; D008826:Microbial Sensitivity Tests; D014295:Trimethoprim / Q000627:therapeutic use*; D041241:Vibrio cholerae O1 / Q000187:drug effects* / Q000302:isolation & purification
chemical_list: D000892:Anti-Infective Agents, Urinary; D002939:Ciprofloxacin; D014295:Trimethoprim