[Flow cytometric basophils activation test as a method of allergy diagnosis].
For several years the incidences of allergic diseases and anaphylactic reactions have been increasing dramatically. Classical method of allergy diagnosis - skin prick test in some situations can provoke life-threatening reactions. Detection of allergen-induced basophil activation by flow cytometry has been shown to be a useful tool for allergy diagnosis in those patients. CD 63 and CD203c have recently been demonstrated as a specific activation markers of basophils that are rapidly up-regulated after allergen challenge in sensitized patients. Although flow-cytometry methods are quite sophisticated and expensive, it could be a good alternative in patients at risk of severe anaphylactic reactions or with contradictory test results.
Potapińska
Olga
O
Zakład Diagnostyki Laboratoryjnej i Immunologii Klinicznej Wieku Rozwojowego Warszawskiego Uniwersytetu Medycznego. olga.potapinska@wum.edu.pl
Demkow
Urszula
U
Wasik
Maria
M
D000485:Allergens / Q000276:immunology*; D039741:Antigens, Dermatophagoides / Q000378:metabolism*; D015549:Basophil Degranulation Test / Q000379:methods; D001491:Basophils / Q000276:immunology; D015415:Biomarkers / Q000378:metabolism; D005434:Flow Cytometry / Q000379:methods; D006801:Humans; D010727:Phosphoric Diester Hydrolases / Q000378:metabolism*; D011058:Pollen / Q000276:immunology; D011755:Pyrophosphatases / Q000378:metabolism*; D012221:Rhinitis, Allergic, Perennial / Q000276:immunology*; D012680:Sensitivity and Specificity
D000485:Allergens; D039741:Antigens, Dermatophagoides; D015415:Biomarkers; C427944:ENPP3 protein, human; D010727:Phosphoric Diester Hydrolases; D011755:Pyrophosphatases