A novel water-soluble beta-(1-->6)-D-glucan isolated from the fruit bodies of Bulgaria inquinans (Fries).
A low molecular-weight polysaccharide named BIWP2 was purified from the fruit bodies of Bulgaria Inquinans (Fries) via hot-water extraction, followed by freeze-thawing and gel filtration chromatography on Sephadex G-75. Monosaccharide composition analysis revealed that BIWP2 contained exclusively glucose. High performance size exclusion chromatography (HPSEC) showed that it was a homogeneous polysaccharide fraction. Its molecular weight was estimated to be 2.6 KD and the polydispersity index (M(w)/M(n)) was calculated to be 1.4. Periodate oxidation, methylation, and NMR analyses indicated that BIWP2 was a linear beta-(1-->6)-D-glucan without side chains. This is the first time to report a linear beta-(1-->6)-D-glucan with low molecular weight in non-lichenized ascomycete.
Bi
Hongtao
H
School of Life Sciences, Northeast Normal University, Changchun 130024, PR China.
Ni
Xiuzhen
X
Liu
Xiaoyu
X
Iteku
Jeff
J
Tai
Guihua
G
Zhou
Yifa
Y
Zhao
Jimin
J
D001203:Ascomycota / Q000737:chemistry*; D002240:Carbohydrate Sequence; D048690:Fruiting Bodies, Fungal / Q000737:chemistry*; D005936:Glucans / Q000737:chemistry* / Q000302:isolation & purification*; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D012995:Solubility; D014867:Water / Q000737:chemistry*; D047071:beta-Glucans / Q000737:chemistry* / Q000302:isolation & purification*
D005936:Glucans; D047071:beta-Glucans; D014867:Water; C064197:beta-1,6-glucan