Sequence selective formation of 1,N(6)-ethenoadenine in DNA by furan-conjugated probe.
1,N(6)-Ethenoadenosine derivatives have been applied as fluorescence probes in various fields of biochemistry and molecular biology. We developed a 1,N(6)-ethenoadenosine-forming reaction at a target adenine in DNA duplex and applied it to a mutation diagnosis. Furan-derivatized oligodeoxyribonucleotides were synthesized and fluorescence properties were studied in the presence of complementary strand under oxidative conditions. Strong emissions at 430nm were observed in the presence of the complementary strand with an adenine in front of furan moiety.
Kobori
Akio
A
Kyoto Institute of Technology, Graduate School of Science and Technology, Department of Biomolecular Engineering, Kyoto, Japan. akobori@kit.ac.jp
Morita
Jumpei
J
Ikeda
Masato
M
Yamayoshi
Asako
A
Murakami
Akira
A
D000225:Adenine / Q000031:analogs & derivatives* / Q000032:analysis / Q000737:chemistry; D000241:Adenosine / Q000031:analogs & derivatives / Q000737:chemistry; D001483:Base Sequence; D004247:DNA / Q000737:chemistry*; D018736:DNA Adducts / Q000032:analysis; D015342:DNA Probes / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry; D005663:Furans / Q000737:chemistry*; D009154:Mutation; D009838:Oligodeoxyribonucleotides / Q000737:chemistry / Q000378:metabolism; D010084:Oxidation-Reduction
D018736:DNA Adducts; D015342:DNA Probes; D005456:Fluorescent Dyes; D005663:Furans; D009838:Oligodeoxyribonucleotides; C004981:1,N(6)-ethenoadenine; C007640:1,N(6)-ethenoadenosine; D004247:DNA; D000225:Adenine; D000241:Adenosine; C039281:furan