The thermo- and acido-stable ORF-99 from the archaeal virus AFV1.
Acidianus Filamentous Virus 1 (AFV1), isolated from acidic hot springs, is an enveloped lipid-containing archaeal filamentous virus with a linear double-stranded DNA genome. It infects Acidianus, which is a hyperthermostable archaea growing at 85 degrees C and acidic pHs, below pH 3. AFV1-99, a protein of 99 amino acids of unknown function, has homologues in the archaeal virus families Lipothrixviridae and Rudiviridae. We determined the crystal structure of AFV1-99 at 2.05 A resolution. AFV1-99 has a new fold, is hyperthermostable (up to 95 degrees C) and resists to extreme pH (between pH 0 and 11) and to the combination of high temperature (95 degrees C) and low pH (pH 0). It possesses characteristics of hyperthermostable proteins, such as a high content of charged residues.
Goulet
Adeline
A
Architecture et Fonction des Macromolécules Biologiques, CNRS and Universités d'Aix-Marseille I & II, 163 avenue de Luminy, Marseille cedex 9, France.
Spinelli
Silvia
S
Blangy
Stéphanie
S
van Tilbeurgh
Herman
H
Leulliot
Nicolas
N
Basta
Tamara
T
Prangishvili
David
D
Cambillau
Christian
C
Campanacci
Valérie
V
D041541:Acidianus / Q000821:virology*; D019943:Amino Acid Substitution; D023641:Archaeal Viruses / Q000737:chemistry* / Q000235:genetics; D003460:Crystallization; D018360:Crystallography, X-Ray; D045482:Hot Springs / Q000382:microbiology / Q000821:virology; D006863:Hydrogen-Ion Concentration; D016366:Open Reading Frames; D011487:Protein Conformation; D013696:Temperature; D014764:Viral Proteins / Q000737:chemistry* / Q000302:isolation & purification
D014764:Viral Proteins