title: Association analyses of a SNP in the promoter of IGF1 with fat deposition and carcass merit traits in hybrid, Angus and Charolais beef cattle.
abstract: A SNP in the promoter region of insulin like growth factor-1 (IGF1) (c.-512C>T) was analysed for associations with 10 fat deposition and carcass merit traits in hybrid (n = 455), Angus (n = 204) and Charolais (n = 186) beef cattle populations. Significant associations of the SNP were found for ultrasound backfat thickness (P = 0.030), carcass average backfat (P = 0.015) and carcass lean meat yield (LMY) (P = 0.023) in the Angus beef population, with the 'CC' genotype showing higher fat depth and lower LMY than the 'TT' genotype. Analyses of transcription factor binding sites based on transcription element search system prediction revealed that the 'C' allele introduces a binding site for nuclear factor I, which has an adipose tissue-specific regulatory role and thus may contribute to the SNP effect on fat deposition in the population of pure Angus cattle, a breed with greater fat depth than the hybrid and Charolais breeds.
authors.affiliation: Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, Alberta T6G 2P5, Canada.
authors.forename: K K
authors.initials: KK
authors.lastname: Islam
authors.forename: M
authors.initials: M
authors.lastname: Vinsky
authors.forename: R E
authors.initials: RE
authors.lastname: Crews
authors.forename: E
authors.initials: E
authors.lastname: Okine
authors.forename: S S
authors.initials: SS
authors.lastname: Moore
authors.forename: D H
authors.initials: DH
authors.lastname: Crews
authors.forename: C
authors.initials: C
authors.lastname: Li
mesh_terms: D000273:Adipose Tissue / Q000033:anatomy & histology*; D000818:Animals; D001823:Body Composition / Q000235:genetics*; D001837:Body Weights and Measures / Q000662:veterinary; D001947:Breeding; D002417:Cattle; D017931:DNA Primers / Q000235:genetics; D056726:Genetic Association Studies / Q000662:veterinary; D007334:Insulin-Like Growth Factor I / Q000235:genetics*; D016014:Linear Models; D020641:Polymorphism, Single Nucleotide / Q000235:genetics; D013045:Species Specificity; D014157:Transcription Factors / Q000378:metabolism
chemical_list: D017931:DNA Primers; D014157:Transcription Factors; D007334:Insulin-Like Growth Factor I