Differential effects of a transgene to confer low phytic acid in caryopses located at different positions in rice panicles.
In previous studies, we attempted to reduce phytic acid in rice seeds by silencing the 1d-myo-inositol 3-phosphate synthase gene, RINO1, using an antisense sequence under the control of the rice glutelin GluB-1 promoter. The stable transgenic line showed a weak low phytic acid phenotype. In this study, we show that the position of the caryopsis in the panicle might affect the level of gene silencing through a difference in temporal and spatial expression patterns between RINO1 and GluB-1 promoters, resulting in a large variation in Pi levels and a small increase in Pi in the transgenic seeds.
Kuwano
Mio
M
Graduate School of Agricultural and Life Sciences, University of Tokyo, Bunkyo-ku, Tokyo, Japan.
Takaiwa
Fumio
F
Yoshida
Kaoru T
KT
D018506:Gene Expression Regulation, Plant; D020868:Gene Silencing; D005983:Glutens / Q000235:genetics / Q000378:metabolism; D019753:Intramolecular Lyases / Q000235:genetics / Q000378:metabolism*; D012275:Oryza / Q000201:enzymology / Q000235:genetics*; D010710:Phosphates / Q000378:metabolism; D010758:Phosphorus / Q000378:metabolism; D010833:Phytic Acid / Q000096:biosynthesis*; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D030821:Plants, Genetically Modified / Q000235:genetics / Q000378:metabolism; D011401:Promoter Regions, Genetic; D012639:Seeds / Q000235:genetics / Q000254:growth & development; D019076:Transgenes
C120054:GluB-1 protein, Oryza; D010710:Phosphates; D010940:Plant Proteins; D010758:Phosphorus; D010833:Phytic Acid; D005983:Glutens; D019753:Intramolecular Lyases; C105497:D-myo-inositol-3-phosphate synthase