A recent adaptive transposable element insertion near highly conserved developmental loci in Drosophila melanogaster.
A recent genomewide screen identified 13 transposable elements that are likely to have been adaptive during or after the spread of Drosophila melanogaster out of Africa. One of these insertions, Bari-Juvenile hormone epoxy hydrolase (Bari-Jheh), was associated with the selective sweep of its flanking neutral variation and with reduction of expression of one of its neighboring genes: Jheh3. Here, we provide further evidence that Bari-Jheh insertion is adaptive. We delimit the extent of the selective sweep and show that Bari-Jheh is the only mutation linked to the sweep. Bari-Jheh also lowers the expression of its other flanking gene, Jheh2. Subtle consequences of Bari-Jheh insertion on life-history traits are consistent with the effects of reduced expression of the Jheh genes. Finally, we analyze molecular evolution of Jheh genes in both the long- and the short-term and conclude that Bari-Jheh appears to be a very rare adaptive event in the history of these genes. We discuss the implications of these findings for the detection and understanding of adaptation.
González
Josefa
J
Department of Biology, Stanford University. jgonzalp@stanford.edu
Macpherson
J Michael
JM
Petrov
Dmitri A
DA
D000483:Alleles; D019943:Amino Acid Substitution / Q000235:genetics; D000818:Animals; D020029:Base Pairing / Q000235:genetics; D001483:Base Sequence; D002470:Cell Survival; D017124:Conserved Sequence; D004251:DNA Transposable Elements / Q000235:genetics*; D021901:DNA, Intergenic / Q000235:genetics; D004331:Drosophila melanogaster / Q000235:genetics* / Q000254:growth & development*; D019143:Evolution, Molecular; D005786:Gene Expression Regulation; D017344:Genes, Insect; D008957:Models, Genetic; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional / Q000235:genetics*; D009154:Mutation / Q000235:genetics; D016366:Open Reading Frames / Q000235:genetics; D010063:Ovum / Q000166:cytology; D010641:Phenotype; D011110:Polymorphism, Genetic; D040641:Quantitative Trait Loci / Q000235:genetics*; D012641:Selection, Genetic
D004251:DNA Transposable Elements; D021901:DNA, Intergenic