PI(3,4,5)P3 Interactome.
Immobilizing chemically synthesized analogues of PI(3,4,5)P3 onto Affi-10 beads and incorporating them into liposomes allowed their use as affinity absorbents in the comprehensive analysis of the phosphoinositide interactome using cytosolic cell extracts of the LIM1215 colon cancer cell line. This led to the identification of 282 proteins that either interact with PI(3,4,5)P3 or are indirectly captured as part of a complex containing a PI(3,4,5)P3 binding partner. Identification of the proteins was achieved using affinity/LC-MS/MS experiments.
Catimel
Bruno
B
Ludwig Institute for Cancer Research, Melbourne Tumour Biology Branch, Royal Melbourne Hospital, and School of Chemistry, Bio21 Institute, University of Melbourne, Parkville, Victoria 3010, Australia. Bruno.catimel@ludwig.edu.au
Yin
Meng-Xin
MX
Schieber
Christine
C
Condron
Melanie
M
Patsiouras
Heather
H
Catimel
Jenny
J
Robinson
Diane E J E
DE
Wong
Leon S-M
LS
Nice
Edouard C
EC
Holmes
Andrew B
AB
Burgess
Antony W
AW
D002277:Carcinoma / Q000378:metabolism*; D045744:Cell Line, Tumor; D003110:Colonic Neoplasms / Q000378:metabolism*; D019295:Computational Biology / Q000379:methods*; D003600:Cytosol / Q000378:metabolism; D005982:Glutathione Transferase / Q000378:metabolism; D006801:Humans; D008081:Liposomes / Q000737:chemistry; D013058:Mass Spectrometry / Q000379:methods; D008956:Models, Chemical; D010710:Phosphates / Q000737:chemistry; D018129:Phosphatidylinositol Phosphates / Q000737:chemistry* / Q000378:metabolism; D025941:Protein Interaction Mapping; D020543:Proteome; D040901:Proteomics / Q000379:methods*
D008081:Liposomes; D010710:Phosphates; D018129:Phosphatidylinositol Phosphates; D020543:Proteome; C060974:phosphatidylinositol 3,4,5-triphosphate; D005982:Glutathione Transferase