Linear disulfide-containing low polymer as efficient DNA cleavage reagent.
A novel linear poly[1,7-bis(mercaptoacetyl)-1,4,7,10-tetraazacyclododecane] (PBMAC) containing macrocyclic polyamine was synthesized through oxidation of 1,7-bis(mercaptoacetyl)-1,4,7,10-tetraazacyclododecane (BMAC) and characterized. Gel electrophoresis experiments showed that PBMAC can promote the DNA cleavage more efficiently than its monomer under physiological conditions without any thiol additives. Fluorescence quenching and DNA melting experiments demonstrated that PBMAC has stronger binding ability with DNA than that of monomer.
Xiang
Yong-Zhe
YZ
Key Laboratory of Green Chemistry and Technology, Ministry of Education, College of Chemistry, Sichuan University, Chengdu 610064, PR China.
Liao
Yi-Le
YL
Zhang
Ji
J
Zhang
Da-Wei
DW
Chen
Shan-Yong
SY
Lu
Qiao-Sen
QS
Zhang
Yu
Y
Lin
Hong-Hui
HH
Yu
Xiao-Qi
XQ
D001372:Aza Compounds / Q000138:chemical synthesis* / Q000737:chemistry; D003516:Cycloparaffins / Q000138:chemical synthesis* / Q000737:chemistry; D053837:DNA Cleavage / Q000187:drug effects*; D004220:Disulfides / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry; D011073:Polyamines / Q000737:chemistry; D011108:Polymers / Q000138:chemical synthesis / Q000737:chemistry* / Q000378:metabolism; D044366:Transition Temperature
D001372:Aza Compounds; D003516:Cycloparaffins; D004220:Disulfides; D005456:Fluorescent Dyes; D011073:Polyamines; D011108:Polymers; C542857:poly(1,7-bis(mercaptoacetyl)-1,4,7,10-tetraazacyclododecane)