title: Association of polymorphisms in the tumor necrosis factor-alpha and interleukin-10 genes with oral lichen planus: a study in a chinese cohort with Han ethnicity.
abstract: Cytokines have an important role in the pathogenesis and disease progression on oral lichen planus (OLP). The aim of this study is to investigate the impact of gene polymorphisms of T-helper cell subtype Th1/Th2 cytokines, tumor necrosis factor-alpha (TNF-alpha), and interleukin-10 (IL-10) on OLP in a Chinese cohort with Han ethnicity. Three single-nucleotide polymorphisms (SNPs) of the IL-10 gene (IL-10) at positions -1082G/A, -819C/T, and -592C/A and one SNP of the TNF-alpha gene (TNFG) at position -308G/A were analyzed in 151 patients with OLP and 143 healthy controls using polymerase chain reaction with sequence-specific primers (PCR-SSP) method and polymerase chain reaction-restriction fragment length (PCR-RFLP) method. The data revealed that there was no difference between the OLP patients and healthy control groups in frequencies of both TNF-alpha and IL-10 alleles (P = 0.053) and genotypes (P = 0.197). However, the frequencies of TNF-alpha-308A allele (P = 0.013) in patients with erosive OLP were significantly greater than the corresponding values in the control group. Using PHASE software, four haplotypes were deduced from the three IL-10 polymorphisms -1082 G/A, -819 C/T, and -592 C/A, named haplotypes I to IV. The haplotypes IV, which correlated with a lower serum level of IL-10, has a significant association with OLP (P = 0.008). Our study suggests that TNF-alpha and IL-10 gene polymorphisms seem to have some influence on the disease susceptibility and progression of OLP in this Chinese cohort with Han ethnicity.
authors.affiliation: Beijing Stomatology Hospital, Capital Medical University, Beijing, People's Republic of China.
authors.forename: Jingping
authors.initials: J
authors.lastname: Bai
authors.forename: Lu
authors.initials: L
authors.lastname: Jiang
authors.forename: Mei
authors.initials: M
authors.lastname: Lin
authors.forename: Xin
authors.initials: X
authors.lastname: Zeng
authors.forename: Zhi
authors.initials: Z
authors.lastname: Wang
authors.forename: Qianming
authors.initials: Q
authors.lastname: Chen
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D044466:Asian People / Q000235:genetics*; D015331:Cohort Studies; D005260:Female; D006801:Humans; D016753:Interleukin-10 / Q000235:genetics* / Q000276:immunology; D017676:Lichen Planus, Oral / Q000208:ethnology / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D011110:Polymorphism, Genetic; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics* / Q000276:immunology; D055815:Young Adult
chemical_list: D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10