Identification and characterization of interferon regulatory factor-1 from orange-spotted grouper (Epinephelus coioides).
Interferon-regulatory factor 1 (IRF-1) is the first member of IRF family, which is involved in many biological processes such as immune response, antiviral defense, cell growth regulation, and apoptosis. In this study, an IRF-1 gene, EcIRF-1, was isolated and characterized from orange-spotted grouper (Epinephelus coioides). The full-length cDNA of EcIRF-1 is 1,730 bp, including an open reading frame of 906 bp, a 5'-terminal untranslated region (5'-UTR) of 153 bp, and a 3'-UTR of 671 bp. The EcIRF-1 gene consists of 10 exons and 9 introns, spanning over approximate 4.3 kb of genomic sequence. The 5'-UTR sequence contains an exon and an intron, and the 3'-UTR sequence is included in the last exon. Expression analysis by real-time PCR reveals that the EcIRF-1 gene is ubiquitously expressed in various healthy fish tissues, whereas its expression is upregulated in vivo in response to polyinosinic-polycytidylic acid or lipopolysaccharide stimulation. Subcellular localization analysis shows the EcIRF-1 is an intranuclearly localized and immobile protein in the cultured fish cells. Data presented in this paper provide an important base to further understand EcIRF-1 gene function and its regulation associated with interferon immune system in orange-spotted grouper.
Shi
Yan
Y
Pearl River Fishery Research Institute, Chinese Academic of Fishery Sciences, Guangzhou, China.
Zhu
Xin-Ping
XP
Yin
Jing-Kui
JK
Zhang
Qi-Ya
QY
Gui
Jian-Fang
JF
D000818:Animals; D001483:Base Sequence; D016000:Cluster Analysis; D017931:DNA Primers / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics; D040461:Gene Components; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000276:immunology*; D050856:Interferon Regulatory Factor-1 / Q000235:genetics* / Q000378:metabolism*; D008070:Lipopolysaccharides; D008969:Molecular Sequence Data; D010473:Perciformes / Q000235:genetics* / Q000276:immunology; D010802:Phylogeny; D011070:Poly I-C; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA
D017931:DNA Primers; D018076:DNA, Complementary; D050856:Interferon Regulatory Factor-1; D008070:Lipopolysaccharides; D011070:Poly I-C