Suicidal function of DNA methylation in age-related genome disintegration.
This article is dedicated to the 60th anniversary of 5-methylcytosine discovery in DNA. Cytosine methylation can affect genetic and epigenetic processes, works as a part of the genome-defense system and has mutagenic activity; however, the biological functions of this enzymatic modification are not well understood. This review will put forward the hypothesis that the host-defense role of DNA methylation in silencing and mutational destroying of retroviruses and other intragenomic parasites was extended during evolution to most host genes that have to be inactivated in differentiated somatic cells, where it acquired a new function in age-related self-destruction of the genome. The proposed model considers DNA methylation as the generator of 5mC>T transitions that induce 40-70% of all spontaneous somatic mutations of the multiple classes at CpG and CpNpG sites and flanking nucleotides in the p53, FIX, hprt, gpt human genes and some transgenes. The accumulation of 5mC-dependent mutations explains: global changes in the structure of the vertebrate genome throughout evolution; the loss of most 5mC from the DNA of various species over their lifespan and the Hayflick limit of normal cells; the polymorphism of methylation sites, including asymmetric mCpNpN sites; cyclical changes of methylation and demethylation in genes. The suicidal function of methylation may be a special genetic mechanism for increasing DNA damage and the programmed genome disintegration responsible for cell apoptosis and organism aging and death.
Mazin
Alexander L
AL
Division of Molecular Basis of Ontogenesis, Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow, Russia. almazin@gmail.com
D044503:5-Methylcytosine / Q000378:metabolism*; D000375:Aging / Q000235:genetics / Q000378:metabolism*; D017209:Apoptosis; D004249:DNA Damage; D019175:DNA Methylation / Q000235:genetics*; D020868:Gene Silencing; D006801:Humans; D009154:Mutation; D012190:Retroviridae / Q000502:physiology
D044503:5-Methylcytosine