title: Effect of mouthrinses on Aggregatibacter actinomycetemcomitans biofilms in a hydrodynamic model.
abstract: The aim of the study was to evaluate the effects of Listerine, Meridol, and Perioaid on the viability and total number of bacteria in established biofilms using an in vitro model under hydrodynamic conditions. Biofilms of Aggregatibacter actinomycetemcomitans were placed in a modified Robbins device and rinsed twice daily during 4 days. Bacteria were quantified by culture and quantitative polymerase chain reaction. Visualization of the samples was performed by scanning electron and confocal laser scanning microscopy, combined with a fluorescent vital staining. All three mouthrinses caused a significant reduction in the number of cultivable A. actinomycetemcomitans in a biofilm. Perioaid was significantly the most powerful in killing the biofilm-protected bacteria and also in counteracting the development of thick dense microbial communities. The total amount of bacteria was not significantly affected by Listerine and Meridol.
authors.affiliation: Research Group for Microbial Adhesion, Department of Periodontology, Catholic University Leuven, Kapucijnenvoer 7, 3000 Leuven, Belgium.
authors.forename: Isabelle
authors.initials: I
authors.lastname: Sliepen
authors.forename: Mark
authors.initials: M
authors.lastname: Van Essche
authors.forename: Marc
authors.initials: M
authors.lastname: Quirynen
authors.forename: Wim
authors.initials: W
authors.lastname: Teughels
mesh_terms: D016976:Aggregatibacter actinomycetemcomitans / Q000187:drug effects* / Q000254:growth & development; D000588:Amines / Q000627:therapeutic use; D000891:Anti-Infective Agents, Local / Q000627:therapeutic use*; D001431:Bacteriological Techniques; D018441:Biofilms / Q000187:drug effects*; D002710:Chlorhexidine / Q000627:therapeutic use; D015169:Colony Count, Microbial; D004338:Drug Combinations; D005456:Fluorescent Dyes; D006801:Humans; D008422:Materials Testing / Q000295:instrumentation; D018613:Microscopy, Confocal; D008855:Microscopy, Electron, Scanning; D009067:Mouthwashes / Q000627:therapeutic use*; D009822:Oils, Volatile / Q000627:therapeutic use; D016133:Polymerase Chain Reaction; D012459:Salicylates / Q000627:therapeutic use; D012703:Serotyping; D013729:Terpenes / Q000627:therapeutic use; D013997:Time Factors; D014002:Tin Fluorides / Q000627:therapeutic use
chemical_list: D000588:Amines; D000891:Anti-Infective Agents, Local; D004338:Drug Combinations; D005456:Fluorescent Dyes; D009067:Mouthwashes; D009822:Oils, Volatile; D012459:Salicylates; D013729:Terpenes; D014002:Tin Fluorides; C059813:Meridol; C057473:Listerine; D002710:Chlorhexidine