title: Extended-spectrum beta-lactamase-producing Escherichia coli in Spain belong to a large variety of multilocus sequence typing types, including ST10 complex/A, ST23 complex/A and ST131/B2.
abstract: In this study, we investigated the population structure of extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli in Spain and determined possible associations between specific multilocus sequence typing (MLST) types and ESBL types. Ninety-two ESBL-producing E. coli isolates from 11 Spanish hospitals were studied. The predominant ESBLs in this collection were CTX-M-14 (45.7%), SHV-12 (21.7%) and CTX-M-9 (20.6%). Phylogenetic groups and MLST types were studied. Thirty-seven isolates (40.2%) belonged to phylogroup A, 26 (28.3%) to group B1, 13 (14.1%) to group B2 and 16 (17.4%) to group D. Fifty-six sequence types (STs) were identified, but ST131 (eight isolates) and ST167 (five isolates) were the most prevalent. The most common ST complexes were ST10 (13 isolates; 14.3%) and ST23 (10 isolates; 11%). Escherichia coli ST131 carried six different ESBLs (CTX-M-1, CTX-M-9, CTX-M-10, CTX-M-14, CTX-M-15 and SHV-12), E. coli ST10 complex carried five ESBLs and E. coli ST23 complex carried four ESBLs. A great diversity of MLST types was observed among Spanish ESBL-producing E. coli isolates.
authors.affiliation: Laboratorio de Antibióticos, Servicio de Bacteriología, Centro Nacional de Microbiología, Instituto de Salud Carlos III, Madrid, Spain.
authors.forename: Jesús
authors.initials: J
authors.lastname: Oteo
authors.forename: Karol
authors.initials: K
authors.lastname: Diestra
authors.forename: Carlos
authors.initials: C
authors.lastname: Juan
authors.forename: Verónica
authors.initials: V
authors.lastname: Bautista
authors.forename: Angela
authors.initials: A
authors.lastname: Novais
authors.forename: María
authors.initials: M
authors.lastname: Pérez-Vázquez
authors.forename: Bartolome
authors.initials: B
authors.lastname: Moyá
authors.forename: Elisenda
authors.initials: E
authors.lastname: Miró
authors.forename: Teresa M
authors.initials: TM
authors.lastname: Coque
authors.forename: Antonio
authors.initials: A
authors.lastname: Oliver
authors.forename: Rafael
authors.initials: R
authors.lastname: Cantón
authors.forename: Ferran
authors.initials: F
authors.lastname: Navarro
authors.forename: José
authors.initials: J
authors.lastname: Campos
mesh_terms: D015373:Bacterial Typing Techniques / Q000379:methods; D016000:Cluster Analysis; D003428:Cross Infection / Q000453:epidemiology / Q000382:microbiology; D016172:DNA Fingerprinting / Q000379:methods; D004926:Escherichia coli / Q000145:classification* / Q000201:enzymology* / Q000235:genetics / Q000302:isolation & purification; D004927:Escherichia coli Infections / Q000453:epidemiology* / Q000382:microbiology*; D005838:Genotype; D006761:Hospitals; D006801:Humans; D017720:Molecular Epidemiology; D015995:Prevalence; D017422:Sequence Analysis, DNA / Q000379:methods; D013030:Spain / Q000453:epidemiology; D001618:beta-Lactamases / Q000096:biosynthesis*
chemical_list: D001618:beta-Lactamases