[Effects of transgenic insect-resistant cotton root exudates on the growth of Verticillium dahliae Kleb].
The decline of disease resistance of transgenic insect-resistant cotton has become one of the important factors restricting China cotton production. With two transgenic insect-resistant cotton varieties and their parental conventional cotton lines as test materials, the effects of their root exudates on the spore germination and mycelial growth of Verticillium dahliae Kleb were studied, and the components and contents of the amino acids and sugars in the exudates were determined. Comparing with their parental lines, the two transgenic insect-resistant varieties had a declined resistance against V. dahliae, and their root exudates had promotion effects on V. dahliae spore germination and mycelial growth. The root exudates of transgenic cotton Zhong-41, compared with those of its parent line Zhong-23, contained two additional amino acids Met and Lys, and the contents of Asp, Glu, Ser, Ala, Val, Leu, and Tyr were significantly higher. Though the components of amino acids in the root exudates of Simian-3 and transgenic cotton GK-12 were the same, the Tyr, Val, and Leu contents in the root exudates of transgenic cotton GK-12 were significantly lower. Four kinds of sugar were detected in the root exudates of Zhong-41, while only glucose was detected in the root exudates of Zhong-23. These four kinds of sugar were also detected in the root exudates of Simian-3 and GK12, but their contents differed significantly.
Li
Xiao-gang
XG
College of Forest Resources and Environment, Nanjing Forestry University, Nanjing 210037, China. sdjnqglxg@163.com
Liu
Biao
B
Liu
Dou-dou
DD
Han
Zheng-min
ZM
Zhou
Ke-xin
KX
Zheng
Yang-ping
YP
D000596:Amino Acids / Q000302:isolation & purification / Q000494:pharmacology; D002241:Carbohydrates / Q000302:isolation & purification / Q000494:pharmacology; D003368:Gossypium / Q000235:genetics*; D010572:Pest Control, Biological; D010935:Plant Diseases; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D018517:Plant Roots / Q000378:metabolism*; D030821:Plants, Genetically Modified; D020172:Verticillium / Q000187:drug effects*
D000596:Amino Acids; D002241:Carbohydrates; D010936:Plant Extracts