title: Nucleotide sequence variation in the hexon gene of human adenovirus type 8 and 37 strains from epidemic keratoconjunctivitis patients in Japan.
abstract: Human adenovirus type 8 (HAdV-8) and 37 (HAdV-37) cause epidemic keratoconjunctivitis (EKC) associated with community-acquired and nosocomial infections. The nucleotide sequences of the entire hexon and fiber genes of eight HAdV-8 and 26 HAdV-37 strains were analysed and the transition mutations in each gene were compared among strains. Compared with prototype strains, the hexon gene of HAdV-8 and -37 strains showed between two and seven and one and twelve variations at nine and 21 different positions, respectively. All of these, except one position in HAdV-37, were located in the conserved region 4 (C4). There were only three polymorphisms in the fiber gene of both HAdV-8 and HAdV-37, fewer than those in C4. The nucleotide sequence of HAdV-8 and -37 C4 might be readily modified during EKC epidemics.
authors.affiliation: Department of Microbiology, Fukushima Medical University School of Medicine, Fukushima, Japan. h-kane@chive.ocn.ne.jp
authors.forename: Hisatoshi
authors.initials: H
authors.lastname: Kaneko
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Ishiko
authors.forename: Takeshi
authors.initials: T
authors.lastname: Ohguchi
authors.forename: Yoshitsugu
authors.initials: Y
authors.lastname: Tagawa
authors.forename: Koki
authors.initials: K
authors.lastname: Aoki
authors.forename: Tatsuo
authors.initials: T
authors.lastname: Suzutani
authors.forename: Shigeaki
authors.initials: S
authors.lastname: Ohno
mesh_terms: D000258:Adenovirus Infections, Human / Q000453:epidemiology / Q000821:virology*; D000260:Adenoviruses, Human / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D001483:Base Sequence; D036022:Capsid Proteins / Q000235:genetics*; D004196:Disease Outbreaks; D014644:Genetic Variation; D006801:Humans; D007564:Japan / Q000453:epidemiology; D007637:Keratoconjunctivitis / Q000453:epidemiology / Q000821:virology*; D008969:Molecular Sequence Data; D009154:Mutation; D010802:Phylogeny
chemical_list: D036022:Capsid Proteins; C018315:hexon capsid protein, Adenovirus