[Antagonism for different doses of taurine on calcium overload in myocardial cells of diastole heart failure rat model].
OBJECTIVE
To study the effects of different doses of taurine (Tau) on calcium ion concentration ([Ca2+]i) and ATPase on cardiocyte membrane of diastole heart failure rats.


METHODS
Diastole heart failure model was established by the coarctation of abdominal aorta. Four weeks after operation, forty diastole heart failure rats were divided into four groups randomly as follows, model (normal saline 2 mL), taurine (400 mg x kg(-1) x d(-1)), taurine (200 mg x kg(-1) x d(-1)), taurine (100 mgx kg(-1) x d(-1)), with 10 rats for each group (n=10), and 10 sham operation rats was taken as control(normal saline, 2 mL). After 4 weeks administration, Isolate single cardiocyte by enzymatic isolation method which were loaded with Ca2+-sensitive fluorescent indicator Fluo-3/AM. [Ca2+]i was measured by laser scanning confocal microscope [LSCM], and represented it by fluorescent intensity [FI]; ATPase activity of cell membrane was measured by the method of enzymatic reaction chromatometry.


RESULTS
Compared with the control group, [Ca2+]i in cardiocyte increased markedly and the ATPase activity of cardiocyte membrane decreased significantly in the model group. Compared with the model group, fluorescent value decreased and ATPase activity increased significantly in Tau high-dose group; fluorescence value and ATPase activity decreased significantly in Tau mid-dose group; fluorescent value decreased and ATPase activity increased significantly in Tau low-dose group.


CONCLUSIONS
Large dosage of Tau can increase ATPase activity on cardiocyte membrane, improve [Ca2+]i in cardiocyte and antagonise calcium overload of DHF rats.
Zhang
Xiaodan
X
School of Pharmacy, Harbin University of Commerce, Harbin 150076, China.
Qu
Yongqing
Y
Zhang
Tingshan
T
Zhang
Qi
Q
D000251:Adenosine Triphosphatases / Q000378:metabolism*; D000818:Animals; D002118:Calcium / Q000378:metabolism*; D003971:Diastole / Q000187:drug effects; D004195:Disease Models, Animal; D005260:Female; D006333:Heart Failure / Q000188:drug therapy / Q000378:metabolism*; D008297:Male; D009206:Myocardium / Q000473:pathology / Q000648:ultrastructure; D032383:Myocytes, Cardiac / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D051381:Rats; D017208:Rats, Wistar; D013654:Taurine / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use
D013654:Taurine; D000251:Adenosine Triphosphatases; D002118:Calcium