[Successful in vitro culture of Plasmodium falciparum gametocytes].
BACKGROUND
The sexual stages of Plasmodium falciparum have not been studied in as much detail as the asexual stages due to the lack of standardized in vitro cultures as well as difficulties in identifying the sexual development stages of the parasite. These difficulties hamper the studies on biology, metabolism, gene expression and protein synthesis during sexual stages. Each of these facets are important targets in antimalarial drug research, particularly the identification of potential therapeutic agents against Plasmodium (derived mainly from plants).


OBJECTIVE
An in vitro culture of P. falciparum gametocytes was established to standardize the identification of its five developmental stages and ensure their continuous production.


METHODS
The in vitro gametocyte culture was established from the P. falciparum NF54 strain in RPMI culture medium, with assessment of the asexual and sexual parasitaemia. The medium was supplemented with type A Rh+ red blood cells only on the first day of culture. Subsequently, the medium was changed daily, together with addition of gas mixture (90% N2, 5% O2, 5% CO2) and maintenance of the culture temperature at 37 degrees C. When asexual parasitaemia reached 3 to 5%, the medium was changed by doubling its volume.


CONCLUSIONS
We standardized an in vitro culture for sexual stages of P. falciparum that can be used for future studies about evaluation of compounds of synthetic or natural origen against the sexual stage, which may permit to develop new control strategies against malaria.
Rada
Ana Mercedes
AM
Grupo Malaria, Universidad de Antioquia, Medellín, Colombia.
Moreno
Carolina
C
Blair
Silvia
S
D000818:Animals; D000962:Antimalarials / Q000494:pharmacology; D018929:Cell Culture Techniques; D002478:Cells, Cultured; D006801:Humans; D020128:Inhibitory Concentration 50; D021261:Parasitic Sensitivity Tests; D010963:Plasmodium falciparum / Q000166:cytology* / Q000187:drug effects / Q000502:physiology
D000962:Antimalarials