title: Macrophage migration inhibitory factor homologs of anisakis simplex suppress Th2 response in allergic airway inflammation model via CD4+CD25+Foxp3+ T cell recruitment.
abstract: We have cloned the macrophage migration inhibitory factor (MIF)-like protein (Anisakis simplex (As)-MIF) from larvae of the whale worm (Anisakis simplex third-stage larvae). Asthma was induced in the mice using OVA/alum, with or without various concentrations of rAs-MIF treatment before OVA/alum challenge. Treatment with rAs-MIF coupled with OVA/alum during the challenge period induced a complete inhibition of eosinophilia and goblet cell hyperplasia within the lung and profoundly ameliorated the development of lung hyperreactivity. Also, rAs-MIF was shown to reduce profoundly the quantity of Th2-related cytokines (IL-4, IL-5, and IL-13) in the bronchial alveolar lavage fluid and allergen-specific IgG2a in sera. IL-10 and TGF-beta levels in the bronchoalveolar lavage fluid of the rAs-MIF-treated group were significantly higher than in the other groups. Additionally, CD4(+)CD25(+)Foxp3(+) T cells (regulatory T) were recruited to the spleen and lungs of the rAs-MIF-treated mice, but this recruitment was inhibited by anti-rAs-MIF Ab.
authors.affiliation: Department of Parasitology, School of Medicine, Pusan National University Busan, Busan, Korea.
authors.forename: Sang Kyun
authors.initials: SK
authors.lastname: Park
authors.forename: Min Kyoung
authors.initials: MK
authors.lastname: Cho
authors.forename: Hye-Kyung
authors.initials: HK
authors.lastname: Park
authors.forename: Keun Hee
authors.initials: KH
authors.lastname: Lee
authors.forename: Sun Joo
authors.initials: SJ
authors.lastname: Lee
authors.forename: Seon Hee
authors.initials: SH
authors.lastname: Choi
authors.forename: Mee Sun
authors.initials: MS
authors.lastname: Ock
authors.forename: Hae Jin
authors.initials: HJ
authors.lastname: Jeong
authors.forename: Moo Hyung
authors.initials: MH
authors.lastname: Lee
authors.forename: Hak Sun
authors.initials: HS
authors.lastname: Yu
mesh_terms: D000818:Animals; D017163:Anisakis; D001249:Asthma / Q000276:immunology*; D001992:Bronchoalveolar Lavage Fluid / Q000276:immunology; D002634:Chemotaxis, Leukocyte / Q000276:immunology*; D016207:Cytokines / Q000032:analysis; D004195:Disease Models, Animal; D007249:Inflammation / Q000276:immunology; D008168:Lung / Q000276:immunology; D008263:Macrophage Migration-Inhibitory Factors / Q000276:immunology*; D051379:Mice; D013154:Spleen / Q000276:immunology; D050378:T-Lymphocytes, Regulatory / Q000276:immunology*; D018418:Th2 Cells / Q000276:immunology*
chemical_list: D016207:Cytokines; D008263:Macrophage Migration-Inhibitory Factors