Effects of nanoparticulate saponin-platinum conjugates on 2,4-dinitrofluorobenzene-induced macrophage inflammatory protein-2 gene expression via reactive oxygen species production in RAW 264.7 cells.
Nanoparticulate platinum (II) (nano Pt) is a powerful antioxidant that is widely used to scavenge reactive oxygen species (ROS). The antioxidant activity of nano Pt has gained attention as a potentially useful therapeutic for a variety of diseases including cancer and aging. In the present study, we prepared nanoparticulate saponin-Pt (II) (nano saponin-Pt) conjugates using the ethanol reduction method to enhance the permeability and retention effect of Pt. The nano saponin-Pt conjugates were found to restore the viability of approximately 40% of 2,4-dinitrofluorobenzene (DNFB)-treated RAW 264.7 cells. In addition, we found that nano saponin-Pt conjugates acted as a potent antioxidant that reduced the production of ROS and inhibited activation of the MAP kinase pathway and MIP-2 gene expression in response to DNFB. These results provide insight into the potential usefulness of nano saponin-Pt conjugates as a treatment for contact hypersensitivity.
Kim
Young-Jin
YJ
Department of Microbiology, College of Medicine, Hallym University, Chuncheon, Korea.
Kim
Dongbum
D
Lee
Younghee
Y
Choi
Soo Young
SY
Park
Jinseu
J
Lee
Seung-Yong
SY
Park
Joon-Won
JW
Kwon
Hyung-Joo
HJ
D000818:Animals; D002460:Cell Line; D054426:Chemokine CXCL2 / Q000235:genetics / Q000378:metabolism*; D004139:Dinitrofluorobenzene / Q000737:chemistry / Q000494:pharmacology*; D004789:Enzyme Activation; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism; D008264:Macrophages / Q000166:cytology / Q000378:metabolism*; D053768:Metal Nanoparticles / Q000737:chemistry*; D051379:Mice; D010984:Platinum / Q000737:chemistry*; D017382:Reactive Oxygen Species / Q000378:metabolism*; D012503:Saponins / Q000737:chemistry*; D048051:p38 Mitogen-Activated Protein Kinases / Q000378:metabolism
D054426:Chemokine CXCL2; D017382:Reactive Oxygen Species; D012503:Saponins; D010984:Platinum; D004139:Dinitrofluorobenzene; D048049:Extracellular Signal-Regulated MAP Kinases; D048051:p38 Mitogen-Activated Protein Kinases