Down-regulation of 67LR reduces the migratory activity of human glioma cells in vitro.
OBJECTIVE
Glioma is the most common brain tumor in central nervous system. Traditional therapies are not effective to cure this disease. Experimental evidence indicates that the 67 kDa elastin-laminin receptor (67LR) subunit is a high-affinity non-integrin laminin-binding protein that is over-expressed on the tumor cell surface in a variety of human carcinomas, and directly correlates with a higher proliferation rate of malignant cells and tendency to metastasize. However, little is known of the expression and function of 67LR in glioma cells.


METHODS
In this study, we estimated whether 67LR was constitutively over-expressed in high-grade astrocytomas by immunohistochemical staining and Western blotting, and investigated the role of a low level of 67LR expression in glioma cell line-U251 by constructing an interfering RNA expression plasmid.


RESULTS
The results showed that the 67LR had an enhanced over-expression in high-grade astrocytomas against normal brain tissues samples, and that the migratory activity of glioma cells was reduced after the down-regulation of the 67LR gene by RNAi.


CONCLUSIONS
It was hypothesized that a low level of 67LR expression could reduce migratory activity of glioma cells, which further proved that 67LR played an important role in glioma invasion by mediating tumor cell functions leading to sarcomata. This study provided a new alternative to gene therapy for glioma treatment.
Chen
F X
FX
School of Life Sciences, Shanghai University, Shanghai 200444, PR China. chenfuxue@staff.shu.edu.cn
Qian
Y R
YR
Duan
Y H
YH
Ren
W W
WW
Yang
Y
Y
Zhang
C C
CC
Qiu
Y M
YM
Ji
Y H
YH
D001254:Astrocytoma / Q000503:physiopathology*; D015153:Blotting, Western; D001921:Brain / Q000378:metabolism; D001932:Brain Neoplasms / Q000503:physiopathology*; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000502:physiology*; D015536:Down-Regulation; D005910:Glioma / Q000503:physiopathology*; D006801:Humans; D007150:Immunohistochemistry; D009367:Neoplasm Staging; D010784:Photomicrography; D034622:RNA Interference; D017474:Receptors, Laminin / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014162:Transfection
D017474:Receptors, Laminin