title: Recombinant Modified Vaccinia Ankara (MVA) effectively boosts DNA-primed HIV-specific immune responses in humans despite pre-existing vaccinia immunity.
abstract: The presence of vector-specific immune responses may hamper the induction of responses to a foreign antigen encoded by the vector. We evaluated the impact of pre-existing immunity to vaccinia virus on the induction of HIV-specific responses after immunization of healthy volunteers with a HIV-1 DNA prime-MVA boost vaccine. Following three priming immunizations with HIV-1 DNA plasmids, the volunteers were boosted with a single injection of recombinant MVA encoding HIV-1 proteins. Pre-existing immunity to vaccinia virus did not reduce the proportion of individuals who responded to HIV-1, but did lower the magnitude of responses. Our results suggest that vaccinia-based vectors can be used to efficiently induce immune responses to vectored HIV-1 antigens, even in individuals with pre-existing immunity to vaccinia virus.
authors.affiliation: Karolinska Institutet and Swedish Institute for Infectious Disease Control, Stockholm, Sweden. L.gudmundsdotter@smi.se
authors.forename: Lindvi
authors.initials: L
authors.lastname: Gudmundsdotter
authors.forename: Charlotta
authors.initials: C
authors.lastname: Nilsson
authors.forename: Andreas
authors.initials: A
authors.lastname: Brave
authors.forename: Bo
authors.initials: B
authors.lastname: Hejdeman
authors.forename: Patricia
authors.initials: P
authors.lastname: Earl
authors.forename: Bernard
authors.initials: B
authors.lastname: Moss
authors.forename: Merlin
authors.initials: M
authors.lastname: Robb
authors.forename: Josephine
authors.initials: J
authors.lastname: Cox
authors.forename: Nelson
authors.initials: N
authors.lastname: Michael
authors.forename: Mary
authors.initials: M
authors.lastname: Marovich
authors.forename: Gunnel
authors.initials: G
authors.lastname: Biberfeld
authors.forename: Eric
authors.initials: E
authors.lastname: Sandström
authors.forename: Britta
authors.initials: B
authors.lastname: Wahren
mesh_terms: D016915:AIDS Vaccines / Q000276:immunology*; D000328:Adult; D000367:Age Factors; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood / Q000276:immunology; D002460:Cell Line; D002522:Chlorocebus aethiops; D005260:Female; D015488:HIV Antigens / Q000276:immunology; D015658:HIV Infections / Q000276:immunology / Q000517:prevention & control*; D015497:HIV-1 / Q000276:immunology*; D006801:Humans; D007111:Immunity, Cellular; D007117:Immunization, Secondary; D008297:Male; D008875:Middle Aged; D009500:Neutralization Tests; D019444:Vaccines, DNA / Q000276:immunology; D014614:Vaccines, Synthetic / Q000276:immunology; D014616:Vaccinia virus / Q000276:immunology*; D055815:Young Adult
chemical_list: D016915:AIDS Vaccines; D000914:Antibodies, Viral; D015488:HIV Antigens; D019444:Vaccines, DNA; D014614:Vaccines, Synthetic