[Clinical significance of lymph vessel density marked by D2-40 in laryngeal squamous cell carcinomas].
OBJECTIVE
To study the relationship between lymphatic vessel density and clinicopathological features of laryngeal squamous cell carcinoma.


METHODS
The lymph vessels, 40 specimens of LSCC and normal mucosa, were quantitated by SABC immunohistochemistry staining with lymphatic endothelial marker oncofetal antigen M2A Monoclonal antibody (D2-40).


RESULTS
The density of peritumoral D2-40(+) vessels in the LSCC group was higher than that of normal mucosa one (P<0.01). The density of peritumoral D2-40(+) vessels in T3/4 group was higher than that of T1/2 group (P<0.05). The density of peritumoral D2-40(+) vessels in the neck lymph nodal metastasis group was higher than that without neck lymph nodal metastasis group (P<0.05). The density of peritumoral D2-40(+) vessels in the supraglottic group was higher than that of glottic group (P<0.01). The density of peritumoral D2-40(+) vessels among high, middle, poor differentiation group were no significant difference (P>0.05).


CONCLUSIONS
Tumor lymph vessel mainly means peritumoral lymph vessel. The density of peritumoral lymph vessel marked with D2-40 in laryngeal carcinoma tissues was significantly correlated with progression and invasion of tumor.
Shuang
Yu
Y
Department of Otolaryngology-Head and Neck Surgery, the Second Affiliated Hospital of Tianjin Medical University, Tianjin, 300211, China. shuangyu618@yahoo.com.cn
Wang
Binquan
B
Wen
Shuxin
S
Huang
Yongwang
Y
D000328:Adult; D000368:Aged; D000911:Antibodies, Monoclonal; D002272:Carcinoembryonic Antigen / Q000276:immunology; D002294:Carcinoma, Squamous Cell / Q000473:pathology*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D007822:Laryngeal Neoplasms / Q000473:pathology*; D008198:Lymph Nodes / Q000473:pathology; D042583:Lymphangiogenesis; D008207:Lymphatic Metastasis; D042601:Lymphatic Vessels / Q000473:pathology*; D008297:Male; D008875:Middle Aged
D000911:Antibodies, Monoclonal; D002272:Carcinoembryonic Antigen