title: CmtR, a cadmium-sensing ArsR-SmtB repressor, cooperatively interacts with multiple operator sites to autorepress its transcription in Mycobacterium tuberculosis.
abstract: CmtR is a repressor of the ArsR-SmtB family from Mycobacterium tuberculosis that has been shown to sense Cd(II) and Pb(II) in Mycobacterium smegmatis. We establish here that CmtR binds cooperatively to multiple sites in M. tuberculosis DNA and protects an unusually long 90 bp AT-rich sequence from -80 bp to +10 with respect to its own initiation codon. CmtR interacts with four hyphenated imperfect inverted repeats matching the consensus sequence TA/GTAA-N(4-5)-TT/GATA in the protected region. SDS-PAGE and formaldehyde crosslinking experiments showed that CmtR forms higher-order oligomers (up to an octamer). The oligomerization of CmtR is in agreement with the cooperative binding of CmtR to multiple sites on DNA. Two promoters transcribe cmtR, and the major promoter physically overlaps with CmtR binding sites. Autorepression of CmtR is mediated by cooperative interaction of CmtR with multiple sites on DNA that occlude the major operon promoter. The combined results of a GFP reporter assay, an electromobility shift assay and a DNase I footprinting experiment establish that Cd(II), not Pb(II), disrupts the interaction of CmtR with DNA to de-repress transcription of the cmtR-Rv1993c-cmtA operon in M. tuberculosis.
authors.affiliation: Department of Biotechnology, All India Institute of Medical Sciences, Ansari Nagar, New Delhi, India.
authors.forename: Santosh
authors.initials: S
authors.lastname: Chauhan
authors.forename: Anil
authors.initials: A
authors.lastname: Kumar
authors.forename: Amit
authors.initials: A
authors.lastname: Singhal
authors.forename: Jaya Sivaswami
authors.initials: JS
authors.lastname: Tyagi
authors.forename: H
authors.initials: H
authors.lastname: Krishna Prasad
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D001483:Base Sequence; D001665:Binding Sites; D002104:Cadmium / Q000378:metabolism*; D018983:DNA Footprinting; D017930:Genes, Reporter; D008969:Molecular Sequence Data; D009169:Mycobacterium tuberculosis / Q000235:genetics / Q000378:metabolism; D009875:Operator Regions, Genetic; D009876:Operon; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D055503:Protein Multimerization; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D012097:Repressor Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D015534:Trans-Activators / Q000235:genetics / Q000378:metabolism*; D024363:Transcription Initiation Site; D014158:Transcription, Genetic
chemical_list: D001426:Bacterial Proteins; D011994:Recombinant Proteins; D012097:Repressor Proteins; D015534:Trans-Activators; D002104:Cadmium