[Preconcentration and separation of DNA fragments based on microchip electrophoresis].
An online preconcentration method, electrokinetic supercharging (EKS) was used for the enrichment of DNA fragments based on microchip electrophoresis (MCE). EKS is a process that combines electrokinetic injection (EKI) with transient isotachophoresis (tITP). The results demonstrated that the large volume of low concentration sample could be introduced, preconcentrated, and eventually separated on a single channel microchip with the whole length of 40.5 mm. The limit of detection (S/N = 3) of DNA fragments was around 0.07 mg/L, effectively improved 40-fold by EKS preconcentration with the normal UV detection at 260 nm. Some important parameters for enhancing preconcentration and qualitative analysis were examined.
Xu
Zhongqi
Z
Department of Chemistry & Chemical Engineering, Graduate School of Engineering, Hiroshima University, Higashi-Hiroshima 739-8527, Japan. chemxzq@hiroshima-u.ac.jp
Hirokawa
Takeshi
T
D004247:DNA / Q000032:analysis*; D046209:Electrophoresis, Microchip; D057625:Isotachophoresis; D057230:Limit of Detection
D004247:DNA