[Apoptosis of colon carcinoma cells of mice induced by vesicular stomatitis virus matrix protein in vitro].
OBJECTIVE
To test the inhibitive effect of the matrix protein of vesicular stomatitis virus (VSV) on the proliferation of colon carcinoma cells of mice in vitro.


METHODS
The plasmid pcDNA3. 1-M encoding M protein of vesicular stomatitis viruses was transfected with lipofectamine 2000 into the colon carcinoma (CT26) cells of mice. The expression of VSV-M protein in the CT26 cells was detected by Western blot. The effect of vesicular stomatitis virus (VSV) matrix protein on the proliferation and apoptosis of colon carcinoma cells was measured by MTT, PI stainning and flow cytometry.


RESULTS
The plasmid expressed M protein in the CT26 cells. Cytopathic effect was shown in the CT26 cells with transfected pcDNA3.1-M. The proliferation of the CT26 cells was suppressed significantly in vitro (76.4%, P < 0.05). The M protein induced apoptosis of the CT26 cells (80.2%), which was higher than'that of the controls (P < 0.05).


CONCLUSIONS
The eukaryotic expression plasmid pcDNA3.1-M encoding M protein of vesicular stomatitis viruses induces apoptosis of CT26 cells, which has implications on the treatment of human colon cancer.
Zhao
Ju-mei
JM
Department of Pharmacology, Medical College of Yan'an University, Yan'an 716000, China.
Liu
Tao
T
Wei
Yu-quan
YQ
Wen
Yan-jun
YJ
Wu
Yang
Y
D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D003110:Colonic Neoplasms / Q000473:pathology*; D015316:Genetic Therapy / Q000379:methods*; D051379:Mice; D010957:Plasmids / Q000235:genetics; D014162:Transfection; D014763:Viral Matrix Proteins / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology*
C094460:M protein, Vesicular stomatitis virus; D014763:Viral Matrix Proteins