title: Dehydrin genes and their expression in recalcitrant oak (Quercus robur) embryos.
abstract: In this work, three dehydrin genes, QrDhn1, QrDhn2, QrDhn3, were isolated from recalcitrant oak (Quercus robur). Their expression pattern was analyzed in both zygotic and somatic embryos as well as in vegetative tissues exposed to different kinds of abiotic stresses including desiccation, osmotic stress, and chilling. The QrDhn1 gene encoding for Y(n)SK(n) type dehydrin was expressed during later stages of zygotic embryo development but in somatic embryos only when exposed to osmotic or desiccation stress. In contrast, the other two oak dehydrin genes encoding for putative K(n) type dehydrins were expressed only in somatic embryos (both not-treated and osmotically stressed) and leaves of oak seedlings exposed to desiccation. Behavior of these genes suggests that different dehydrins are involved in processes of seed maturation and response to altered osmotic (water status) conditions in somatic embryos. Revealing further members of dehydrin gene family in recalcitrant oak might contribute to clarify non-orthodox seed behavior as well as identify mechanisms contributing to desiccation tolerance in plants.
authors.affiliation: Institute of Plant Genetics and Biotechnology, Slovak Academy of Sciences, Akademická 2, P.O. Box 39A, 950 07, Nitra 1, Slovak Republic.
authors.forename: Vanda
authors.initials: V
authors.lastname: Sunderlíková
authors.forename: Ján
authors.initials: J
authors.lastname: Salaj
authors.forename: Dieter
authors.initials: D
authors.lastname: Kopecky
authors.forename: Terézia
authors.initials: T
authors.lastname: Salaj
authors.forename: Eva
authors.initials: E
authors.lastname: Wilhem
authors.forename: Ildikó
authors.initials: I
authors.lastname: Matusíková
mesh_terms: D000595:Amino Acid Sequence; D018744:DNA, Plant / Q000235:genetics; D003890:Desiccation; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D029963:Quercus / Q000235:genetics* / Q000378:metabolism; D012639:Seeds / Q000235:genetics / Q000378:metabolism*; D016415:Sequence Alignment
chemical_list: D018744:DNA, Plant; D010940:Plant Proteins; C070556:dehydrin proteins, plant