Multicolor quantum dot-encoded microspheres for the fluoroimmunoassays of chicken newcastle disease and goat pox virus.
Semiconductor nanocrystals (or quantum dots, QDs) have the potential to overcome some of the limitations encountered by traditional fluorophores in fluorescence labeling applications. The unique spectroscopic properties of QDs make them hold immense promise as versatile labels for biological applications. In this work, we employ the layer-by-layer (LbL) method for the construction of bio-functional multicolor QD-encoded microspheres. Polystyrene microspheres with diameter of 3 microm were used as templates for the deposition of different sized CdTe QDs/polyelectrolyte multilayers. Two different antigens, Chicken newcastle disease (CND) antigen and goat pox virus (GPV) antigen, were conjugated to two kinds of biofunctional multicolor microspheres with different optical encoding. The multicolor microspheres can capture corresponding antibodies labeled with QDs, QDs-CND antibody and QDs-GPV antibody in the fluoroimmunoassays. The microspheres can be distinguished from each other based on their optical encoding.
Yuan
Pingfan
P
Department of Analytical Chemistry, College of Chemistry, Jilin University, Changchun 130012, China.
Ma
Qiang
Q
Meng
Rizeng
R
Wang
Chao
C
Dou
Wenchao
W
Wang
Guannan
G
Su
Xingguang
X
D000818:Animals; D000956:Antigens, Viral / Q000032:analysis* / Q000276:immunology; D019187:Cadmium Compounds / Q000138:chemical synthesis / Q000737:chemistry*; D018151:Capripoxvirus / Q000276:immunology / Q000302:isolation & purification*; D002645:Chickens; D015200:Fluoroimmunoassay / Q000379:methods*; D018796:Immunoconjugates / Q000737:chemistry / Q000276:immunology; D008863:Microspheres; D009522:Newcastle disease virus / Q000276:immunology / Q000302:isolation & purification*; D011137:Polystyrenes / Q000138:chemical synthesis / Q000737:chemistry*; D045663:Quantum Dots; D013050:Spectrometry, Fluorescence; D013691:Tellurium / Q000737:chemistry*
D000956:Antigens, Viral; D019187:Cadmium Compounds; D018796:Immunoconjugates; D011137:Polystyrenes; D013691:Tellurium; C028337:cadmium telluride