title: Reduction of sample evaporation in small volume microplate luminescence assays.
abstract: Luminescence detection has great potential for use in small volume assays for high-throughput applications. However, the need for automated injection of detection reagents dictates an open well format, making small volume luminescence assays very susceptible to sample evaporation effects. Here we describe a technique for reducing evaporation of small sample volumes that uses layering of silicone oil on solution surfaces but still allows the use of automated injection.
authors.affiliation: Sci-Tec Inc., Knoxville, TN 37932, USA. klvngrgry@hotmail.com
authors.forename: Kalvin J
authors.initials: KJ
authors.lastname: Gregory
authors.forename: Yeh
authors.initials: Y
authors.lastname: Sun
mesh_terms: D008163:Luminescent Measurements / Q000379:methods*; D012827:Silicone Oils / Q000494:pharmacology; D014835:Volatilization
chemical_list: D012827:Silicone Oils