Isolation of highly enriched apical plasma membranes of the placental syncytiotrophoblast.
The human placenta is a complex organ whose proper function is crucial for the development of the fetus. The placenta contains within its structure elements of the maternal and fetal circulatory systems. The interface with maternal blood is the lining of the placenta, that is a unique compartment known as the syncytiotrophoblast. This large syncytial structure is a single cell layer in thickness, and the apical plasma membrane of the syncytiotrophoblast interacts directly with maternal blood. Relatively little is known about the proteins that reside in this unique plasma membrane or how they may change in various placental diseases. Our goal was to develop methods for isolating highly enriched preparations of this apical plasma membrane compatible with high-quality proteomics analysis and herein describe the properties of these isolated membranes.
Robinson
John M
JM
Department of Physiology and Cell Biology, Ohio State University, 1645 Neil Ave., 304 Hamilton Hall, Columbus, OH 43210, USA. robinson.21@osu.edu
Ackerman
William E
WE
Tewari
Arun K
AK
Kniss
Douglas A
DA
Vandre
Dale D
DD
D002458:Cell Fractionation / Q000379:methods*; D002462:Cell Membrane / Q000737:chemistry / Q000378:metabolism* / Q000648:ultrastructure; D003102:Colloids; D005260:Female; D018922:HL-60 Cells; D006801:Humans; D008854:Microscopy, Electron; D010920:Placenta / Q000166:cytology*; D011247:Pregnancy; D040901:Proteomics; D012822:Silicon Dioxide; D014327:Trophoblasts / Q000166:cytology*
D003102:Colloids; D012822:Silicon Dioxide