Improving the catalytic performance of fungal laccases in monoterpene-based reaction systems.
Ternary systems consisting of monoterpenes (alpha-pinene or D-limonene), tert-butanol and water were used as reaction media to enhance the catalytic performance of laccases from various fungi sources (Trametes versicolor, T. hirsuta and Botrytis cinerea). The enzymes had improved catalytic efficiency (5- to 10-fold) in alpha-pinene-rich environment, while optimal reaction rates were in high-water content systems (15.5% v/v). The stability of laccases was significantly improved in monoterpene-based systems (up to 90% residual enzyme activity after 24 h at 30 degrees C) in comparison with other non-conventional media. The results indicate that these ternary systems can increase the potential of laccases as catalysts for various oxidations.
Tzialla
Aikaterini A
AA
Biotechnology Laboratory, Department of Biological Applications and Technologies, University of Ioannina, 45110 Ioannina, Greece.
Taha
Ali A
AA
Kalogeris
Emmanuel
E
Stamatis
Haralambos
H
D020171:Botrytis / Q000201:enzymology*; D004795:Enzyme Stability; D005656:Fungal Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D042845:Laccase / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D039821:Monoterpenes / Q000378:metabolism*; D012997:Solvents / Q000494:pharmacology; D013696:Temperature; D013997:Time Factors; D055454:Trametes / Q000201:enzymology*; D014867:Water / Q000494:pharmacology; D020002:tert-Butyl Alcohol / Q000378:metabolism
D005656:Fungal Proteins; D039821:Monoterpenes; D012997:Solvents; D014867:Water; D042845:Laccase; D020002:tert-Butyl Alcohol