title: Transferrin secretory pathways in rat parotid acinar cells.
abstract: Transferrin is the major iron transporter in blood plasma, and is also found, at lower concentrations, in saliva. We studied the synthesis and secretion of transferrin in rat parotid acinar cells in order to elucidate its secretory pathways. Two sources were identified for transferrin in parotid acinar cells: synthesis by the cells (endogenous), and absorption from blood plasma (exogenous). Transferrin from both sources is secreted from the apical side of parotid acinar cells. Endogenous transferrin is transported to secretory granules. It is secreted from mature secretory granules upon stimulation with a beta-adrenergic reagent and from smaller vesicles in the absence of stimulation. Exogenous transferrin is internalized from the basolateral side of parotid acinar cells, transported to the apical side by transcytosis, and secreted from the apical side. Secretory processes for exogenous transferrin include transport systems involving microfilaments and microtubules.
authors.affiliation: Department of Biochemistry, The Nippon Dental University, School of Life Dentistry at Niigata, Hamaura-cho, Niigata, Japan. nashida@ngt.ndu.ac.jp
authors.forename: Tomoko
authors.initials: T
authors.lastname: Nashida
authors.forename: Sumio
authors.initials: S
authors.lastname: Yoshie
authors.forename: Akane
authors.initials: A
authors.lastname: Imai
authors.forename: Hiromi
authors.initials: H
authors.lastname: Shimomura
mesh_terms: D000818:Animals; D003599:Cytoskeleton / Q000378:metabolism; D005786:Gene Expression Regulation; D010306:Parotid Gland / Q000166:cytology* / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D011990:Receptors, Transferrin / Q000378:metabolism; D055571:Secretory Pathway; D022142:Secretory Vesicles / Q000378:metabolism; D014168:Transferrin / Q000235:genetics / Q000378:metabolism*
chemical_list: D012333:RNA, Messenger; D011990:Receptors, Transferrin; D014168:Transferrin