Field assessment of an H5N1 inactivated vaccine in chickens and ducks in Lao PDR.
Despite the extensive use of poultry vaccines to control the spread of H5N1 influenza in poultry, H5N1 outbreaks continue to occur in domestic birds. Our objective was to determine the duration of the neutralizing antibody response under field conditions after vaccination with a laboratory-tested inactivated reverse genetics-derived H5N3 vaccine. H5N3 hemagglutination inhibition (HI) and virus neutralization (VN) antibodies were observed 40 weeks after vaccination of chickens with two doses and vaccination of ducks with one dose. Cross-clade antibodies to an H5N1 virus (A/chicken/Laos/A0464/07) antigenically distinct from the vaccine strain were detected in ducks after a single vaccination and were sustained for 28 weeks (for 40 weeks when a boost vaccination was given). Our results indicate that this inactivated H5N3 vaccine can produce long-lasting antibodies to homologous and heterologous viruses under field conditions.
Boltz
David A
DA
Department of Infectious Diseases, Division of Virology, St. Jude Children's Research Hospital, 262 Danny Thomas Place, Memphis, TN 38105-3678, USA.
Douangngeun
Bounlom
B
Sinthasak
Settha
S
Phommachanh
Phouvong
P
Midouangchanh
Phetlamphone
P
Walker
David
D
Keating
Rachael
R
Khalenkov
Alexey M
AM
Kumar
Mahesh
M
Webster
Robert G
RG
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D002645:Chickens; D003429:Cross Reactions; D004372:Ducks; D006385:Hemagglutination Inhibition Tests; D053124:Influenza A Virus, H5N1 Subtype / Q000276:immunology*; D007252:Influenza Vaccines / Q000276:immunology*; D005585:Influenza in Birds / Q000517:prevention & control*; D007812:Laos; D009500:Neutralization Tests; D015164:Vaccines, Inactivated / Q000276:immunology
D000914:Antibodies, Viral; D007252:Influenza Vaccines; D015164:Vaccines, Inactivated