title: Prolonged venoarterial extracorporeal membrane oxygenation after transplantation restores functional integrity of severely injured lung allografts and prevents the development of pulmonary graft failure in a pig model.
abstract: OBJECTIVE
  Prolonged venoarterial extracorporeal membrane oxygenation support during transplantation provides reduction of pulmonary artery flow and allows for protective ventilation. This approach might have the potential to restore function of lungs that would be unsuitable for transplantation.
  
  
  METHODS
  Left lung transplantation was performed on 16 pigs. Lungs from brain-dead animals were stored for 22 hours at 4 degrees C. Recipients in group A (n = 8) underwent transplantation without cardiopulmonary support followed by ventilation with 10 mL/kg body weight tidal volume. Animals in group B (n = 8) underwent transplantation during venoarterial extracorporeal membrane oxygenation, which was continued for 22 hours, and received low-tidal-volume (5 mL/kg body weight) ventilation. One hour after transplantation, the right lung was excluded. Graft function was compared immediately after exclusion of the contralateral lung (time point 1), 1 hour later (time point 2), and 1 hour after discontinuation of extracorporeal membrane oxygenation (time point 3).
  
  
  RESULTS
  Four animals in group A did not reach time point 2; all died of pulmonary edema. All animals in group B survived, and at time point 3, the mean Pao(2) value was 323 +/- 129 mm Hg. At time point 2, oxygenation and lung compliance were higher in group B than in group A, whereas pulmonary artery pressure was lower. The same was true when comparing results of group B at time point 3 with results of group A at time point 2.
  
  
  CONCLUSIONS
  Transplantation during extracorporeal membrane oxygenation with continued use for 24 hours restores function of damaged donor lungs. This could expand the donor pool through wider use of marginal donors.
authors.affiliation: Department of Cardiothoracic Surgery, Medical University of Vienna, Vienna, Austria.
authors.forename: György
authors.initials: G
authors.lastname: Lang
authors.forename: Clemens
authors.initials: C
authors.lastname: Aigner
authors.forename: Günther
authors.initials: G
authors.lastname: Winkler
authors.forename: Keso
authors.initials: K
authors.lastname: Shkirdladze
authors.forename: Wilfried
authors.initials: W
authors.lastname: Wisser
authors.forename: Gerhard
authors.initials: G
authors.lastname: Dekan
authors.forename: Masaya
authors.initials: M
authors.lastname: Tamura
authors.forename: Georg
authors.initials: G
authors.lastname: Heinze
authors.forename: Dirk
authors.initials: D
authors.lastname: Van Raemdonck
authors.forename: Walter
authors.initials: W
authors.lastname: Klepetko
mesh_terms: D000818:Animals; D001794:Blood Pressure; D015199:Extracorporeal Membrane Oxygenation; D006085:Graft Survival; D007430:Intraoperative Care; D016040:Lung Transplantation; D009926:Organ Preservation; D011182:Postoperative Care; D011651:Pulmonary Artery; D011652:Pulmonary Circulation; D011654:Pulmonary Edema / Q000517:prevention & control*; D015427:Reperfusion Injury / Q000517:prevention & control; D034421:Sus scrofa