Broad range RT-PCR assays targeting human noroviruses also detect swine noroviruses.
Conventional broad range RT-PCR and real time RT-PCR assays designed to detect human noroviruses (NoVs) also efficiently detect swine NoVs. Investigation of the primers and probe binding sites revealed strong homologies between swine NoVs genomic sequences and human primer sequences. These findings have a serious impact on food diagnostic methods and laboratories.
L'Homme
Yvan
Y
Canadian Food Inspection Agency, St-Hyacinthe Laboratory, 3400 Blvd Casavant West, St-Hyacinthe, Quebec J2S 8E3, Canada. yvan.l'homme@inspection.gc.ca
Sansregret
Raphaël
R
Simard
Carole
C
D000818:Animals; D001483:Base Sequence; D017250:Caliciviridae Infections / Q000175:diagnosis* / Q000821:virology; D017931:DNA Primers; D005506:Food Contamination / Q000032:analysis*; D005516:Food Microbiology; D005759:Gastroenteritis / Q000175:diagnosis* / Q000821:virology; D005784:Gene Amplification; D006801:Humans; D029322:Norovirus / Q000145:classification / Q000302:isolation & purification*; D012367:RNA, Viral / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods / Q000592:standards; D012680:Sensitivity and Specificity; D016415:Sequence Alignment; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity; D013552:Swine
D017931:DNA Primers; D012367:RNA, Viral