title: Laboratory appraisal of optimal gaseous conditions for growth of zoonotic Helicobacter felis ATCC 49179.
abstract: An attempt was made to assess the hitherto undescribed optimal gaseous conditions for growth of zoonotic Helicobacter felis, focusing on the ratio of spiral-forms amongst the whole cells examined. The largest mean colony diameter was obtained under the gaseous condition of O(2) 12% and CO(2) 10%. In analyzing the five day old colonies, the highest percentage of spiral forms (85.5%) was observed under the condition of O(2) 18% and CO(2) 5%. In contrast, the lowest percentage of spiral forms (2.3%) was demonstrated under the condition of O(2) 1% and CO(2) 10%. The condition of O(2) 12% and CO(2) 10% was concluded to be optimal for obtaining cells with the largest colony sizes, although colonies proliferated under such conditions definitely contain many more coccoid cells than spiral forms. In culturing H. felis strains, optimal gaseous conditions should be employed according to the purposes or preferences of study designs.
authors.affiliation: Department of Laboratory Medicine, Shinshu University Graduate School of Medicine, Matsumoto, Japan.
authors.forename: Mayumi
authors.initials: M
authors.lastname: Shiohara
authors.forename: Masatomo
authors.initials: M
authors.lastname: Kawakubo
authors.forename: Takehisa
authors.initials: T
authors.lastname: Matsumoto
authors.forename: Toshiko
authors.initials: T
authors.lastname: Kumagai
authors.forename: Kazuyoshi
authors.initials: K
authors.lastname: Yamauchi
authors.forename: Kozue
authors.initials: K
authors.lastname: Oana
authors.forename: Hiroyoshi
authors.initials: H
authors.lastname: Ota
authors.forename: Yoshiyuki
authors.initials: Y
authors.lastname: Kawakami
mesh_terms: D000818:Animals; D002245:Carbon Dioxide / Q000378:metabolism*; D005740:Gases / Q000378:metabolism; D016481:Helicobacter Infections / Q000382:microbiology*; D044922:Helicobacter felis / Q000166:cytology / Q000254:growth & development* / Q000378:metabolism; D006801:Humans; D010100:Oxygen / Q000378:metabolism*; D015047:Zoonoses / Q000382:microbiology*
chemical_list: D005740:Gases; D002245:Carbon Dioxide; D010100:Oxygen