title: Inhibition of adipocyte differentiation by RORalpha.
abstract: Here we show that gene expression of the nuclear receptor RORalpha is induced during adipogenesis, with RORalpha4 being the most abundantly expressed isoform in human and murine adipose tissue. Over-expression of RORalpha4 in 3T3-L1 cells impairs adipogenesis as shown by the decreased expression of adipogenic markers and lipid accumulation, accompanied by decreased free fatty acid and glucose uptake. By contrast, mouse embryonic fibroblasts from staggerer mice, which carry a mutation in the RORalpha gene, differentiate more efficiently into mature adipocytes compared to wild-type cells, a phenotype which is reversed by ectopic RORalpha4 restoration.
authors.affiliation: Institut Pasteur de Lille, Département d'Athérosclérose, Lille F-59019, France.
authors.forename: Hélène
authors.initials: H
authors.lastname: Duez
authors.forename: Christian
authors.initials: C
authors.lastname: Duhem
authors.forename: Saara
authors.initials: S
authors.lastname: Laitinen
authors.forename: Prashant S
authors.initials: PS
authors.lastname: Patole
authors.forename: Mouaadh
authors.initials: M
authors.lastname: Abdelkarim
authors.forename: Brigitte
authors.initials: B
authors.lastname: Bois-Joyeux
authors.forename: Jean-Louis
authors.initials: JL
authors.lastname: Danan
authors.forename: Bart
authors.initials: B
authors.lastname: Staels
mesh_terms: D041721:3T3-L1 Cells; D017667:Adipocytes / Q000166:cytology* / Q000378:metabolism*; D050156:Adipogenesis / Q000235:genetics / Q000502:physiology; D000328:Adult; D000818:Animals; D002454:Cell Differentiation / Q000235:genetics / Q000502:physiology; D005230:Fatty Acids, Nonesterified / Q000378:metabolism; D015870:Gene Expression; D005947:Glucose / Q000378:metabolism; D006801:Humans; D066298:In Vitro Techniques; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008818:Mice, Neurologic Mutants; D057094:Nuclear Receptor Subfamily 1, Group F, Member 1; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D018160:Receptors, Cytoplasmic and Nuclear / Q000172:deficiency / Q000235:genetics / Q000378:metabolism*; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D015534:Trans-Activators / Q000172:deficiency / Q000235:genetics / Q000378:metabolism*
chemical_list: D005230:Fatty Acids, Nonesterified; D057094:Nuclear Receptor Subfamily 1, Group F, Member 1; D012333:RNA, Messenger; C539738:RORA protein, human; D018160:Receptors, Cytoplasmic and Nuclear; D011994:Recombinant Proteins; D015534:Trans-Activators; D005947:Glucose