Lipoprotein lipase but not hormone-sensitive lipase activities achieve normality after surgically induced weight loss in morbidly obese patients.
BACKGROUND
Although bariatric surgery is currently the most common practice for inducing weight loss in morbidly obese patients (BMI>40 kg/m2), its effect on the lipid content of adipose tissue and its lipases (lipoprotein lipase [LPL] and hormone-sensitive lipase [HSL]) are controversial.


METHODS
We analyzed LPL and HSL activities and lipid content from plasma as well as subcutaneous (SAT) and visceral (VAT) adipose tissue of 34 morbidly obese patients (MO) before and after (6 and 12 months) Roux-en-Y gastric bypass surgery and compare the values with those of normal weight (control) patients.


RESULTS
LPL activity was significantly higher in MO (SAT=32.9+/-1.0 vs VAT=36.4+/-3.3 mU/g tissue; p<0.001) than in control subjects (SAT=8.2+/-1.4 vs VAT=6.8+/-1.0 mU/g tissue) in both adipose depots. HSL activity had similar values in both types of tissue (SAT=32.8+/-1.6 and VAT=32.9+/-1.6 mU/g) of MO. In the control group, we found similar results but with lower values (SAT=11.9+/-1.4 vs VAT=12.1+/-1.4 mU/g tissue). Twelve months after surgery, SAT LPL activity diminished (9.8+/-1.4 mU/g tissue, p<0.001 vs morbidly obese), while HSL (46.6+/-3.7 mU/g tissue) remained high. All lipids in tissue and plasma diminished after bariatric surgery except plasma nonesterified fatty acids, which maintained higher levels than controls (16+/-3 vs 9+/-0 mg/dL; p<0.001, respectively).


CONCLUSIONS
When obese patients lose weight, they lose not only part of the lipid content of the cells but also the capacity to store triacylglycerides in SAT depots.
Pardina
E
E
Biochemistry and Molecular Biology Department, Biology Faculty, Universitat de Barcelona, Diagonal 645, 08028, Barcelona, Spain.
Lecube
A
A
Llamas
R
R
Catalán
R
R
Galard
R
R
Fort
J M
JM
Allende
H
H
Vargas
V
V
Baena-Fustegueras
J A
JA
Peinado-Onsurbe
J
J
D000328:Adult; D016022:Case-Control Studies; D005260:Female; D005500:Follow-Up Studies; D015390:Gastric Bypass; D006801:Humans; D050152:Intra-Abdominal Fat / Q000378:metabolism / Q000473:pathology; D050356:Lipid Metabolism; D008071:Lipoprotein Lipase / Q000097:blood*; D008297:Male; D008875:Middle Aged; D009767:Obesity, Morbid / Q000503:physiopathology / Q000601:surgery*; D002787:Sterol Esterase / Q000097:blood*; D050151:Subcutaneous Fat / Q000378:metabolism / Q000473:pathology; D016896:Treatment Outcome; D015431:Weight Loss
D002787:Sterol Esterase; D008071:Lipoprotein Lipase