Using simultaneous repetitive Transcranial Magnetic Stimulation/functional Near Infrared Spectroscopy (rTMS/fNIRS) to measure brain activation and connectivity.
BACKGROUND
Simultaneously acquiring functional Near Infrared Spectroscopy (fNIRS) during Transcranial Magnetic Stimulation (rTMS) offers the possibility of directly investigating superficial cortical brain activation and connectivity. In addition, the effects of rTMS in distinct brain regions without quantifiable behavioral changes can be objectively measured.


METHODS
Healthy, nonmedicated participants age 18-50 years were recruited from the local community. After written informed consent was obtained, the participants were screened to ensure that they met inclusion criteria. They underwent two visits of simultaneous rTMS/fNIRS separated by 2 to 3 days. In each visit, the motor cortex and subsequently the prefrontal cortex (5 cm anterior to the motor cortex) were stimulated (1 Hz, max 120% MT, 10 s on with 80 s off, for 15 trains) while simultaneous fNIRS data were acquired from the ipsilateral and contralateral brain regions.


RESULTS
Twelve healthy volunteers were enrolled with one excluded prior to stimulation. The 11 participants studied (9 male) had a mean age of 31.8 (s.d. 10.2, range 20-49) years. There was no significant difference in fNIRS between Visit 1 and Visit 2. Stimulation of both the motor and prefrontal cortices resulted in a significant decrease in oxygenated hemoglobin (HbO(2)) concentration in both the ipsilateral and contralateral cortices. The ipsilateral and contralateral changes showed high temporal consistency.


CONCLUSIONS
Simultaneous rTMS/fNIRS provides a reliable measure of regional cortical brain activation and connectivity that could be very useful in studying brain disorders as well as cortical changes induced by rTMS.
Kozel
F Andrew
FA
Department of Psychiatry, University of Texas Southwestern Medical Center, Dallas, TX 75390-9119, USA. Andrew.Kozel@utsouthwestern.edu
Tian
Fenghua
F
Dhamne
Sameer
S
Croarkin
Paul E
PE
McClintock
Shawn M
SM
Elliott
Alan
A
Mapes
Kimberly S
KS
Husain
Mustafa M
MM
Liu
Hanli
H
D000293:Adolescent; D000328:Adult; D001931:Brain Mapping / Q000379:methods; D002540:Cerebral Cortex / Q000502:physiology*; D005071:Evoked Potentials / Q000502:physiology*; D005260:Female; D006454:Hemoglobins / Q000032:analysis*; D006801:Humans; D008297:Male; D008875:Middle Aged; D009415:Nerve Net / Q000502:physiology*; D009434:Neural Pathways / Q000502:physiology*; D019265:Spectroscopy, Near-Infrared / Q000379:methods*; D050781:Transcranial Magnetic Stimulation / Q000379:methods*; D055815:Young Adult
D006454:Hemoglobins