title: Effects of two estradiol regimens on anxiety and depressive behaviors and trophic effects in peripheral tissues in a rodent model.
abstract: BACKGROUND
  With aging and menopause, which are associated with decreases in ovarian steroids such as 17beta-estradiol (E(2)), women might experience negative psychological symptoms, including anxiety and depression. Some women use E(2)-based therapies to alleviate these symptoms, but E(2) has been associated with trophic effects that might increase vulnerability to some steroid-sensitive cancers, such as breast cancer, in both premenopausal and postmenopausal women.
  
  
  OBJECTIVE
  This study investigated the relationships between the possible beneficial effects of E(2) on anxiety and depressive behaviors concurrent with trophic effects using an animal model of E(2) decline and replacement.
  
  
  METHODS
  Dose-dependent effects of E(2) on affective, sexual, and motor behavior of young adult rats were studied. Ovariectomized (OVX) rats were administered the chemical carcinogen 7,12-dimethylbenz(a) anthracene (DMBA) 1.25 mg or inactive vehicle (vegetable oil; control) by gavage. E(2) (0.03 or 0.09 mg/kg) or vehicle was administered subcutaneously 44 to 48 hours before assessments of anxiety (light-dark transition), depression (forced swim test), sexual (lordosis), and motor (activity monitor) behaviors. Fourteen weeks after carcinogen exposure, E(2) concentrations in plasma and brain regions (cortex, hippocampus, and hypothalamus) were determined. Incidences and numbers of tumors and uterine weight were analyzed.
  
  
  RESULTS
  Administration of E(2) (0.09 mg/kg) was associated with significant increases in antianxiety-like behavior in the light-dark transition task, antidepressant-like behavior in the forced swim test, and physiologic circulating and central E(2) concentrations compared with E(2) (0.03 mg/kg) and vehicle. Compared with vehicle, E(2) (0.9 > 0.3 mg/kg) was associated with significant increases in lordosis and uterine weight. Administration of DMBA was associated with significant increases in the incidences and numbers of tumors; this effect was augmented by E(2)administration.
  
  
  CONCLUSIONS
  Based on the findings in this rat model, the hypothesis that E(2) may be effective in reducing anxiety and depressive behaviors and enhance sexual behavior in OVX rats, concurrent with trophic effects in the periphery, was supported. Moderate physiologic levels of E(2) might have beneficial effects on affective and sexual behaviors in female rodents, but regimens including E(2) might increase tumorigenic capacity.
authors.affiliation: Department of Psychology, The Centers for Neuroscience and Life Sciences Research, The University at Albany-State University of New York, Albany, New York 12222, USA.
authors.forename: Alicia A
authors.initials: AA
authors.lastname: Walf
authors.forename: Cheryl A
authors.initials: CA
authors.lastname: Frye
mesh_terms: D000818:Animals; D001007:Anxiety / Q000097:blood / Q000150:complications / Q000188:drug therapy*; D001522:Behavior, Animal / Q000187:drug effects; D001921:Brain / Q000187:drug effects; D003863:Depression / Q000097:blood / Q000150:complications / Q000188:drug therapy*; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D004958:Estradiol / Q000008:administration & dosage* / Q000009:adverse effects / Q000097:blood; D004967:Estrogens / Q000008:administration & dosage* / Q000009:adverse effects / Q000097:blood; D005080:Exercise Test / Q000187:drug effects; D005260:Female; D009369:Neoplasms / Q000139:chemically induced / Q000473:pathology; D009929:Organ Size / Q000187:drug effects; D017698:Postmenopause / Q000187:drug effects*; D051381:Rats; D047368:Tumor Burden / Q000187:drug effects; D014599:Uterus / Q000187:drug effects / Q000473:pathology
chemical_list: D004967:Estrogens; D004958:Estradiol