title: [Detection of antimalarial activity for new compounds by isotopic microtest].
abstract: Two clone lines (Dd2 and 3D7) of Plasmodium falciparum were cultivated continuously in human erythrocytes at 37 degrees C in RPMI 1640 medium with human serum and subjected to 6% sorbitol treatment 2 times in order to obtain highly synchronized cultures. The second generation parasites after the treatment were diluted with human RBC to be a suspension of P. falciparum-human RBC at 2.5% hematocrit and 0.5% parasitemia, and 2 microCi/ml of 8-3H-hypoxanthine was added. Isotopic microtest was employed to detect the antimalarial activity for 20 new compounds. Results revealed that the 20 compounds showed no anti-malarial activity, while the control drugs, chloroquine and quinine, exhibited high efficacy, indicating that the isotopic microtest is a stable and reproducible assay for screening new antimalarials.
authors.affiliation: Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Key Laboratory of Animal Parasitology, Ministry of Agriculture, Shanghai 200232, China. zhaoguochen70@yahoo.com.cn
authors.forename: Zhao-guo
authors.initials: ZG
authors.lastname: Chen
authors.forename: Alicia
authors.initials: A
authors.lastname: Moreno
authors.forename: Agustin
authors.initials: A
authors.lastname: Benito
authors.forename: Marta
authors.initials: M
authors.lastname: Moreno
authors.forename: Pedro J
authors.initials: PJ
authors.lastname: Berzosa
authors.forename: Aida
authors.initials: A
authors.lastname: de Lucio
authors.forename: Eva
authors.initials: E
authors.lastname: Moyano
mesh_terms: D000962:Antimalarials / Q000032:analysis* / Q000494:pharmacology*; D004353:Drug Evaluation, Preclinical; D004912:Erythrocytes / Q000469:parasitology; D006801:Humans; D021261:Parasitic Sensitivity Tests; D010963:Plasmodium falciparum / Q000187:drug effects*
chemical_list: D000962:Antimalarials