title: Chaperone Skp from Yersinia pseudotuberculosis exhibits immunoglobulin G binding ability.
abstract: A low-molecular-weight cationic protein that can bind human and rabbit immunoglobulins G has been isolated from Yersinia pseudotuberculosis cells. This immunoglobulin binding protein (IBP) interacts with IgG Fc-fragment, the association constant of the resulting complex being 3.1 microM(-1). MALDI-TOF mass spectrometry analysis of IBP revealed its molecular mass of 16.1 kDa, and capillary isoelectrofocusing analysis showed pI value of 9.2. N-Terminal sequence determination by Edman degradation revealed the sequence of the 15 terminal amino acid residues (ADKIAIVNVSSIFQ). Tryptic hydrolysate of IBP was subjected to MALDI-TOF mass spectrometry for proteolytic peptide profiling. Based on the peptide fingerprint, molecular mass, pI, and N-terminal sequence and using bioinformatic resources, IBP was identified as Y. pseudotuberculosis periplasmic chaperone Skp. Using the method of comparative modeling a spatial model of Skp has been built. This model was then used for modeling of Skp complexes with human IgG1 Fc-fragment by means of molecular docking.
authors.affiliation: Pacific Institute of Bioorganic Chemistry, Far East Division of the Russian Academy of Sciences, Vladivostok, 690022, Russia. sev1972@mail.ru
authors.forename: E V
authors.initials: EV
authors.lastname: Sidorin
authors.forename: R H
authors.initials: RH
authors.lastname: Ziganshin
authors.forename: G A
authors.initials: GA
authors.lastname: Naberezhnykh
authors.forename: G N
authors.initials: GN
authors.lastname: Likhatskaya
authors.forename: E V
authors.initials: EV
authors.lastname: Trifonov
authors.forename: S D
authors.initials: SD
authors.lastname: Anastiuk
authors.forename: O V
authors.initials: OV
authors.lastname: Chernikov
authors.forename: T F
authors.initials: TF
authors.lastname: Solov'eva
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D007074:Immunoglobulin G / Q000378:metabolism*; D008958:Models, Molecular; D018832:Molecular Chaperones / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D011485:Protein Binding; D015011:Yersinia pseudotuberculosis / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D007074:Immunoglobulin G; D018832:Molecular Chaperones