Interleukin-21 regulates expression of the immediate-early lytic cycle genes and proteins in Epstein-Barr Virus infected B cells.
The switch between the latent and the lytic cycle of Epstein-Barr Virus (EBV) infection is characterized by the induction of viral transcriptional activators Zta and Rta. CD4(+) T cell-derived cytokines are likely to regulate expression of these proteins in EBV-infected B cells. Here we show that interleukin-21 decreases constitutive expression of Rta and its target EA-D in EBV-infected B cell lines during the first 3 days of culture. In some cell lines this is followed by a strong increase in the expression of Zta, Rta, and EA-D during prolonged culture. Additionally, we provide evidence that IL-21-mediated JAK/STAT signals regulate increase in the Zta expression.
Konforte
Danijela
D
Division of Stem Cell and Developmental Biology, Princess Margaret Hospital, Ontario Cancer Institute, University Health Network, 610 University Ave. #8-105, M5G 2M9 Toronto, Canada. danijela.konforte@utoronto.ca
Paige
Christopher J
CJ
D001402:B-Lymphocytes / Q000821:virology*; D002460:Cell Line; D015967:Gene Expression Regulation, Viral; D017781:Genes, Immediate-Early; D004854:Herpesvirus 4, Human / Q000502:physiology*; D054884:Host-Pathogen Interactions; D006801:Humans; D017874:Immediate-Early Proteins / Q000096:biosynthesis; D007378:Interleukins / Q000276:immunology*; D015534:Trans-Activators / Q000096:biosynthesis; D000098546:Interleukin-21
C068382:BRLF1 protein, Human herpesvirus 4; C063676:BZLF1 protein, Herpesvirus 4, Human; D017874:Immediate-Early Proteins; D007378:Interleukins; D015534:Trans-Activators; D000098546:Interleukin-21