title: An evaluation of extracts of five traditional medicinal  plants from Iran on the inhibition of mushroom  tyrosinase activity and scavenging of free radicals.
abstract: This study aimed to evaluate the free radical scavenging and inhibition properties of five medicinal plants, including Quercus infectoria Olive., Terminalia chebula Retz., Lavendula stoechas L., Mentha longifolia L., Rheum palmatum L., toward the activity of mushroom tyrosinase using L-tyrosine and L-3,4-dihydroxyphenylalanine (L-DOPA) as the substrate.The methanol extracts of Q. infectoria and T. chebula showed strong radical scavenging effect in 2,2'-dipheny L-1-picrylhydrazyl (DPPH) assay(IC50 = 15.3 and 82.2 microg mL)1 respectively).These plants also showed inhibitory effects against the activity of mushroom tyrosinase in hydroxylation of L-tyrosine (85.9% and 82.2% inhibition,respectively). These two plants also inhibited the oxidation of l-DOPA similar to kojic acid as positive control (IC50 = 102.8 and 192.6 microg mL)1 respectively). In general Q. infectoria and T. chebula significantly inhibited tyrosinase activity and DPPH radical. Both activities were concentration dependant but not in linear manner. It is needed to study the cytotoxicity of these plant extracts in pigment cell culture before further evaluation and moving to in vivo conditions.
authors.affiliation: Department of Pharmaceutics, Research Center of Pharmaceutics, Kerman University of Medical Sciences, Kerman, Iran.
authors.forename: P
authors.initials: P
authors.lastname: Khazaeli
authors.forename: R
authors.initials: R
authors.lastname: Goldoozian
authors.forename: F
authors.initials: F
authors.lastname: Sharififar
mesh_terms: D000363:Agaricales / Q000201:enzymology*; D000975:Antioxidants / Q000494:pharmacology; D001713:Biphenyl Compounds / Q000378:metabolism; D016166:Free Radical Scavengers / Q000494:pharmacology*; D020128:Inhibitory Concentration 50; D007492:Iran; D007980:Levodopa / Q000378:metabolism; D014442:Monophenol Monooxygenase / Q000037:antagonists & inhibitors* / Q000378:metabolism; D010851:Picrates / Q000378:metabolism; D010936:Plant Extracts / Q000494:pharmacology*; D010946:Plants, Medicinal / Q000737:chemistry*
chemical_list: D000975:Antioxidants; D001713:Biphenyl Compounds; D016166:Free Radical Scavengers; D010851:Picrates; D010936:Plant Extracts; D007980:Levodopa; C004931:1,1-diphenyl-2-picrylhydrazyl; D014442:Monophenol Monooxygenase