[Identification of Wnt/beta-catenin signal pathway in human prostate cancer cell lines and its significance].
OBJECTIVE
To determine the Wnt/beta-catenin signal pathway in different human prostate cancer cell lines and explore its role in epithelial-mesenchymal transition (EMT) in human prostate cancer.


METHODS
We detected the expressions of beta-catenin, t-GSK3beta and p-GSK3beta in several prostate cancer cell lines (LNCaP, C4, C4-2, C4-2B, IF11, IA8, PC-3 and DU145) with different characteristics of epithelial-mesenchymal transition (EMT) by Western blotting.


RESULTS
There were remarkable differences in the expressions of beta-catenin and p-GSK3beta among the cell lines, with beta-catenin and p-GSK3beta highly expressed in LNCaP, C4, C4-2, C4-2B, IF11 and IA8, lowly expressed in PC-3 and DU145, but no difference was observed in the expressions of t-GSK3beta in all the cell lines.


CONCLUSIONS
There are differences in the state of the Wnt/beta-catenin signal pathway among the cell lines with different characters of EMT.
Jiang
Yong-Guang
YG
Department of Urology, Beijing Anzhen Hospital Affiliated to Capital Medical University, Beijing 100029, China. yongguangjiang@yahoo.com.cn
Zhao
Jia-Hui
JH
Luo
Yong
Y
He
Da-Lin
DL
Wang
Xin-Yang
XY
Cui
Xin-Hao
XH
D045744:Cell Line, Tumor; D006801:Humans; D008297:Male; D011471:Prostatic Neoplasms / Q000378:metabolism*; D015398:Signal Transduction; D051153:Wnt Proteins / Q000378:metabolism*; D051176:beta Catenin / Q000378:metabolism*
D051153:Wnt Proteins; D051176:beta Catenin