Toxicity of fluoride to microorganisms in biological wastewater treatment systems.
Fluoride is a common contaminant in a variety of industrial wastewaters. Available information on the potential toxicity of fluoride to microorganisms implicated in biological wastewater treatment is very limited. The objective of this study was to evaluate the inhibitory effect of fluoride towards the main microbial populations responsible for the removal of organic constituents and nutrients in wastewater treatment processes. The results of short-term batch bioassays indicated that the toxicity of sodium fluoride varied widely depending on the microbial population. Anaerobic microorganisms involved in various metabolic steps of anaerobic digestion processes were found to be very sensitive to the presence of fluoride. The concentrations of fluoride causing 50% metabolic inhibition (IC(50)) of propionate- and butyrate-degrading microorganisms as well as mesophilic and thermophilic acetate-utilizing methanogens ranged from 18 to 43 mg/L. Fluoride was also inhibitory to nitrification, albeit at relatively high levels (IC(50)=149 mg/L). Nitrifying bacteria appeared to adapt rapidly to fluoride, and a near complete recovery of their metabolic activity was observed after only 4d of exposure to high fluoride levels (up to 500 mg/L). All other microbial populations evaluated in this study, i.e., glucose fermenters, aerobic glucose-degrading heterotrophs, denitrifying bacteria, and H(2)-utilizing methanogens, tolerated fluoride at very high concentrations (>500 mg/L).
Ochoa-Herrera
Valeria
V
Department of Chemical and Environmental Engineering, University of Arizona, P.O. Box 210011, Tucson, AZ 85721-0011, USA.
Banihani
Qais
Q
León
Glendy
G
Khatri
Chandra
C
Field
James A
JA
Sierra-Alvarez
Reyes
R
D000332:Aerobiosis / Q000187:drug effects; D000693:Anaerobiosis / Q000187:drug effects; D001419:Bacteria / Q000187:drug effects* / Q000378:metabolism; D002087:Butyrates / Q000378:metabolism; D005285:Fermentation / Q000187:drug effects; D005459:Fluorides / Q000633:toxicity*; D005947:Glucose / Q000378:metabolism; D052836:Heterotrophic Processes / Q000187:drug effects; D008697:Methane / Q000378:metabolism; D009584:Nitrogen / Q000378:metabolism; D009930:Organic Chemicals / Q000378:metabolism; D011422:Propionates / Q000378:metabolism; D012722:Sewage / Q000737:chemistry / Q000382:microbiology; D018675:Toxicity Tests; D014865:Waste Disposal, Fluid; D014874:Water Pollutants, Chemical / Q000633:toxicity*; D018508:Water Purification
D002087:Butyrates; D009930:Organic Chemicals; D011422:Propionates; D012722:Sewage; D014874:Water Pollutants, Chemical; D005947:Glucose; D009584:Nitrogen; D008697:Methane; D005459:Fluorides