Characterization of the constitutive heterochromatin of Astyanax sp. D (Characiformes: Characidae) from the Upper Iguaçu River (PR).
Chromosomal characterization of constitutive heterochromatin was performed in Astyanax sp. D, a species found in the Iguaçu river, using C-banding, fluorescent in situ hybridization (FISH) of the repetitive DNA family As51, localization of the 18S rDNA sites and base-specific fluorochromes. The sites of the satellite DNA coincide with large heterochromatic blocks present in this species, namely CMA3- and DAPI-. The many 18S rDNA sites are CMA3+. Similarity between the large heterochromatic blocks is suggested, as well as the identification of two types of heterochromatin, one related to the 18S rDNA domains and the other to the As51 regions.
Kantek
Daniel Luis Zanella
DL
Laboratório de Biodiversidade Molecular e Citogenética, Universidade Federal de São Carlos, CEP 13566-430, São Carlos, SP, Brazil. daniel_kantek@hotmail.com
Cestari
Marta Margarete
MM
Bertollo
Luiz Antônio Carlos
LA
Moreira-Filho
Orlando
O
D000818:Animals; D001938:Brazil; D002874:Chromosome Mapping; D004275:DNA, Ribosomal / Q000235:genetics; D004276:DNA, Satellite / Q000235:genetics; D005260:Female; D005399:Fishes / Q000235:genetics*; D006570:Heterochromatin / Q000235:genetics*; D017404:In Situ Hybridization, Fluorescence; D007621:Karyotyping; D008297:Male
D004275:DNA, Ribosomal; D004276:DNA, Satellite; D006570:Heterochromatin