[Embolization and degradation of arteries in vivo by pingyangmycin albumin microspheres].
OBJECTIVE
To investigate the embolization effect of Pingyangmycin-albumin microspheres (PYM-AMS) on small arteries and its process of degradation in vivo.


METHODS
Twenty four Japanese white rabbits were randomly divided into four groups, 6 in each group. PYM hydrochloride + 0.9% NaCl, PYM + soybean oil, and PYM-AMS + soybean oil were injected into the central auricular arteries of the rabbits in the three experimental groups, respectively, for about 30 seconds (0.26 mL/per ear, which contained PYM 5 mg/mL). The vessel samples were taken and examined at 2, 7, 14, and 21 days.


RESULTS
The PYM + 0.9% NaCl group had no significant vessel changes. In the PYM+ soybean oil group, some endothelial cells dropped off at the 7th day after injection. At the 21st day, mild proliferation of endothelial cells and walls of central auricular arteries were observed, especially on the intima. But the lumen was still obvious and the blood flow was not blocked. In the PYM-AMS group, the central auricular arteries were narrowed at the 7th day after injection. At the 21st day, the vessels had sclerostenosis, and the blood flow was blocked. At the 14th day, significant proliferation of endothelial cells and walls of central auricular arteries were observed. The surface of PYM-AMS was absorbed. At the 21st day, the walls of central auricular arteries and some small veins proliferated obviously, and the arteries were sclerostenosed. Many smooth muscle cells, fibroblasts, and myofibroblasts in the original blood vessel lumen appeared. There were thrombi besides the PYM-AMS.


CONCLUSIONS
PYM-AMS may become an option for the treatment of large venous or arteriovenous malformations and for the local chemotherapy of malignant tumors.
Wang
Li-li
LL
Laboratory of Oral Diseases, Department of Head and Neck Oncology, West China Stomatological Hospital, Sichuan University, Chengdu 610041, China.
Gao
Qing-hong
QH
Zheng
Gen-jian
GJ
Wang
Xiao-yi
XY
Zhou
Lan
L
Wang
Chang-mei
CM
Wen
Yu-ming
YM
D000818:Animals; D000903:Antibiotics, Antineoplastic / Q000008:administration & dosage*; D001761:Bleomycin / Q000008:administration & dosage / Q000031:analogs & derivatives*; D016461:Chemoembolization, Therapeutic / Q000379:methods*; D042783:Endothelial Cells / Q000187:drug effects* / Q000473:pathology; D005260:Female; D008297:Male; D008863:Microspheres; D011817:Rabbits; D011897:Random Allocation; D012710:Serum Albumin, Bovine / Q000737:chemistry
D000903:Antibiotics, Antineoplastic; D001761:Bleomycin; D012710:Serum Albumin, Bovine; C025703:bleomycetin