[Establishment of a rat model of bone marrow mesenchymal stem cell transplantation for repairing full-thickness skin defect].
OBJECTIVE
To establish a rat model of full-thickness skin defect to receive bone marrow mesenchymal stem cell transplantation for wound repair.


METHODS
A full-thickness skin defect measuring 4 cmx4 cm in 36 F344 rats, which were divided into 3 groups with the wound covered with alloskin graft, acellular dermal matrix, or petrolatum gauze. In vitro cultured BMSCs in the 5th passage were transplanted into the skin defect, and the time of wound dressing dissociation and number of transplanted Brdu-positive cells in the wound were observed 14 days later.


RESULTS
The alloskin graft resulted in significantly longer time before dressing dissociation, with greater number of Brdu-positive cells in the wound than the other two wound dressings (P<0.001). The acellular dermal matrix showed better effect than petrolatum gauze in terms of the dressing dissociation time and the viable transplanted cell number in the wound.


CONCLUSIONS
Alloskin graft can be ideal for covering the wound surface to protect the transplanted BMSCs in rats.
Xiong
Bing
B
Department of Plastic Surgery, Nangfang Hospital, Southern Medical University, Guangzhou 510515, China. xiongbing1@hotmail.com
Gao
Jian-hua
JH
Chen
Hua-de
HD
Lu
Feng
F
Wei
Li-jun
LJ
Lai
Wen
W
Zheng
Shao-yi
SY
Gao
Hui
H
Bian
Hui-ning
HN
Liu
Zu-an
ZA
Huang
Zhi-feng
ZF
Sun
Chuan-wei
CW
Lin
Qiu-xiong
QX
D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology; D020405:Dermis / Q000637:transplantation; D005260:Female; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation; D011897:Random Allocation; D051381:Rats; D011916:Rats, Inbred F344; D017208:Rats, Wistar; D012867:Skin / Q000293:injuries*; D014184:Transplantation, Homologous; D014945:Wound Healing / Q000502:physiology