title: Emergence of Pseudomonas aeruginosa strains producing metallo-beta-lactamases of the IMP-15 and VIM-2 types in Mexico.
abstract: Eighty-six carbapenem non-susceptible Pseudomonas aeruginosa isolates collected in the National Institute of Respiratory Diseases of Mexico City were screened for the presence of metallo-beta-lactamase (MBL) activity using both E-test strips and a microbiological assay with EDTA-imipenem. Genomic comparisons and sequence analyses conducted with these isolates revealed the presence of bla(VIM-2) in two clonally related isolates, and bla(IMP-15) in a clonally unrelated isolate. Both genes were found to be carried by class 1 integrons, and bla(IMP-15) was additionally present on a broad host-range plasmid. This is the first report of co-existing P. aeruginosa strains producing different MBLs in a Mexican hospital, highlighting the necessity of appropriate surveillance to prevent dissemination of carbapenem resistance.
authors.affiliation: Servicio de Microbiología Clínica, Instituto Nacional de Enfermedades Respiratorias, Mexico City, Mexico.
authors.forename: F
authors.initials: F
authors.lastname: Quinones-Falconi
authors.forename: M
authors.initials: M
authors.lastname: Galicia-Velasco
authors.forename: P
authors.initials: P
authors.lastname: Marchiaro
authors.forename: M A
authors.initials: MA
authors.lastname: Mussi
authors.forename: V
authors.initials: V
authors.lastname: Ballerini
authors.forename: A J
authors.initials: AJ
authors.lastname: Vila
authors.forename: A M
authors.initials: AM
authors.lastname: Viale
authors.forename: K
authors.initials: K
authors.lastname: Bermejo-Morales
authors.forename: A S
authors.initials: AS
authors.lastname: Limansky
mesh_terms: D001426:Bacterial Proteins / Q000096:biosynthesis* / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D006761:Hospitals; D006801:Humans; D032023:Integrons; D008800:Mexico; D008826:Microbial Sensitivity Tests / Q000379:methods; D010957:Plasmids; D011552:Pseudomonas Infections / Q000382:microbiology*; D011550:Pseudomonas aeruginosa / Q000201:enzymology* / Q000302:isolation & purification*; D001618:beta-Lactamases / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; C425108:beta-lactamase bla(vim-2); D001618:beta-Lactamases; C547882:beta-lactamase IMP-15, Pseudomonas aeruginosa