[Establishment of a prion disease PrP(Sc) panel from the brain tissues of experimental hamsters infected with scrapie agent 263K].
OBJECTIVE
To establish a prion disease PrP(Sc) panel from the brain tissues of experimental hamsters and to address the stability of the panel conserved under the specific condition, for evaluating the diagnostic techniques of human and animal's prion diseases.


METHODS
30 brain tissues of hamsters infected with scrapie strain 263K intracerebrally and 30 ones of normal hamsters were enrolled in this panel. Each brain sample was prepared to 10%, 1% and 0.5% homogenates and aliquoted into stocks. The presences of PrP(Sc) in each brain sample were evaluated with PrP-specific Western Blots and partially with immunohistochemistry, and the stability of PrP(Sc) signals in each sample were repeatedly assessed half a year later and 3 years later.


RESULTS
PrP(Sc) signals were detected in all stocks of 10% brain homogenates from infected hamsters, 26 out of 30 stocks of 1% homogenates and 19 out of 30 stocks of 0.5% homogenates. The assessments of PrP(Sc) signals in all samples half-year and three years later demonstrated almost unchanged. All homogenates of brain tissues of normal hamsters were PrP(Sc) negative.


CONCLUSIONS
A prion disease PrP(Sc) panel of the brain tissues, which includes 90 PrP(Sc) positive stocks and PrP(Sc) negative ones, was successfully established, with a reliable stability of PrP(Sc) signals.
Zhang
Bao-yun
BY
State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, 100052, China.
Tian
Chan
C
Han
Jun
J
Gao
Chen
C
Shi
Qi
Q
Jiang
Hui-ying
HY
Zhou
Wei
W
Dong
Xiao-ping
XP
D000818:Animals; D001921:Brain / Q000378:metabolism; D004195:Disease Models, Animal; D006801:Humans; D008297:Male; D018621:PrPC Proteins / Q000493:pharmacokinetics*; D018620:PrPSc Proteins; D017096:Prion Diseases / Q000378:metabolism*; D012608:Scrapie / Q000378:metabolism*; D014018:Tissue Distribution
D018621:PrPC Proteins; D018620:PrPSc Proteins