Identification of three immunodominant regions on leukotoxin protein of Fusobacterium necrophorum.
To analyze immunodominant regions of leukotoxin protein of Fusobacterium necrophorum strain H05, a series of truncated forms of leukotoxin gene were expressed in Escherichia coli using the vector pGEX-6p-1 or pPROEX HTa. The results of SDS-PAGE showed the truncated forms PL1, PL2, PL4, and PL5 were expressed in Escherichia coli using the vector pGEX-6p-1, and the truncated forms PL3 was expressed in Escherichia coli using the vector pPROEX HTa. These recombinant proteins were able to react with antisera against Fusobacterium necrophorum strain A25. In five recombinant proteins, the recombinant proteins PL1, PL3 and PL4 as vaccine were able to elicit formation of the better protective effects on mice against infection of Fusobacterium necrophorum strain A25.
Sun
D B
DB
College of Animal Science and Technology, Heilongjiang August First Land Reclamation University, Daqing High-tech Industrial Development Zone, Daqing, 163319, People's Republic of China.
Wu
R
R
Li
G L
GL
Zheng
J S
JS
Liu
X P
XP
Lin
Y C
YC
Guo
D H
DH
D000818:Animals; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology / Q000627:therapeutic use; D001427:Bacterial Toxins / Q000737:chemistry / Q000276:immunology*; D003603:Cytotoxins / Q000737:chemistry / Q000276:immunology; D017931:DNA Primers; D004926:Escherichia coli / Q000235:genetics; D005098:Exotoxins / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D005535:Foot Rot / Q000276:immunology* / Q000517:prevention & control / Q000635:transmission; D005673:Fusobacterium / Q000235:genetics / Q000276:immunology* / Q000472:pathogenicity; D005822:Genetic Vectors; D006460:Hemolysin Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology / Q000627:therapeutic use; D007166:Immunosuppressive Agents / Q000737:chemistry / Q000276:immunology*; D011994:Recombinant Proteins / Q000276:immunology; D012418:Ruminants; D014774:Virulence
D001426:Bacterial Proteins; D001427:Bacterial Toxins; D003603:Cytotoxins; D017931:DNA Primers; D005098:Exotoxins; D006460:Hemolysin Proteins; D007166:Immunosuppressive Agents; D011994:Recombinant Proteins; C018579:leukotoxin; C102677:lktA protein, bacteria