A nucleolar protein RRS1 contributes to chromosome congression.
We report here the functional analysis of human Regulator of Ribosome Synthesis 1 (RRS1) protein during mitosis. We demonstrate that RRS1 localizes in the nucleolus during interphase and is distributed at the chromosome periphery during mitosis. RNA interference experiments revealed that RRS1-depleted cells show abnormalities in chromosome alignment and spindle organization, which result in mitotic delay. RRS1 knockdown also perturbs the centromeric localization of Shugoshin 1 and results in premature separation of sister chromatids. Our results suggest that a nucleolar protein RRS1 contributes to chromosome congression.
Gambe
Arni E
AE
Department of Biotechnology, Graduate School of Engineering, Osaka University, Suita, Osaka, Japan.
Matsunaga
Sachihiro
S
Takata
Hideaki
H
Ono-Maniwa
Rika
R
Baba
Akiko
A
Uchiyama
Susumu
S
Fukui
Kiichi
K
D001483:Base Sequence; D018797:Cell Cycle Proteins / Q000378:metabolism; D002466:Cell Nucleolus / Q000378:metabolism / Q000648:ultrastructure; D023902:Chromosome Pairing; D020090:Chromosome Segregation; D002877:Chromosomes, Human / Q000378:metabolism* / Q000648:ultrastructure; D006367:HeLa Cells; D006801:Humans; D008856:Microscopy, Fluorescence; D008938:Mitosis; D009687:Nuclear Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics; D016601:RNA-Binding Proteins; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D008941:Spindle Apparatus / Q000378:metabolism / Q000648:ultrastructure
D018797:Cell Cycle Proteins; D009687:Nuclear Proteins; D034741:RNA, Small Interfering; D016601:RNA-Binding Proteins; C405526:RRS1 protein, human; D011994:Recombinant Proteins; C496644:SGO1 protein, human