title: Corosolic acid induces apoptosis through mitochondrial pathway and caspase activation in human cervix adenocarcinoma HeLa cells.
abstract: We investigated the response of human cervix adenocarcinoma HeLa cells to Corosolic acid (CRA) treatment. Our results showed that CRA significantly inhibited cell viability in both a dose- and a time-dependent manner. CRA treatment induced S cell-cycle arrest and caused apoptotic death in HeLa cells. We found that CRA increased in Bax/Bcl-2 ratios by up-regulating Bax expression, disrupted mitochondrial membrane potential and triggered the release of cytochrome c from mitochondria into the cytoplasm. Moreover, CRA treatment triggered the activation of caspase-8, -9 and -3 in HeLa cells. All these results indicate that CRA-induced apoptosis is associated with the activation of caspases via a mitochondrial pathway. Taken together, we believe that CRA could have strong potentials for clinical application in treating human cervix adenocarcinoma and improving cancer chemotherapy.
authors.affiliation: Department of Traditional Chinese Medicine, Changhai Hospital, Second Military Medical University, Shanghai 200433, People's Republic of China.
authors.forename: Yanfeng
authors.initials: Y
authors.lastname: Xu
authors.forename: Ruiliang
authors.initials: R
authors.lastname: Ge
authors.forename: Juan
authors.initials: J
authors.lastname: Du
authors.forename: Hailiang
authors.initials: H
authors.lastname: Xin
authors.forename: Tingjiao
authors.initials: T
authors.lastname: Yi
authors.forename: Jiayu
authors.initials: J
authors.lastname: Sheng
authors.forename: Yongzi
authors.initials: Y
authors.lastname: Wang
authors.forename: Changquan
authors.initials: C
authors.lastname: Ling
mesh_terms: D029042:Actinidia / Q000737:chemistry; D000230:Adenocarcinoma / Q000201:enzymology / Q000473:pathology*; D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D020169:Caspases / Q000502:physiology*; D004354:Drug Screening Assays, Antitumor; D004365:Drugs, Chinese Herbal / Q000737:chemistry; D004789:Enzyme Activation / Q000187:drug effects; D005260:Female; D006367:HeLa Cells; D006801:Humans; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D008928:Mitochondria / Q000187:drug effects* / Q000502:physiology; D015394:Molecular Structure; D009363:Neoplasm Proteins / Q000502:physiology*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000032:analysis; D016196:S Phase / Q000187:drug effects; D014315:Triterpenes / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D002583:Uterine Cervical Neoplasms / Q000201:enzymology / Q000473:pathology*; D051028:bcl-2-Associated X Protein / Q000032:analysis
chemical_list: D000972:Antineoplastic Agents, Phytogenic; C494826:BAX protein, human; D004365:Drugs, Chinese Herbal; D009363:Neoplasm Proteins; D019253:Proto-Oncogene Proteins c-bcl-2; D014315:Triterpenes; D051028:bcl-2-Associated X Protein; C113861:corosolic acid; D020169:Caspases