Evaluation of a Western Blot and ELISA for the detection of anti-Trichinella-IgG in pig sera.
Human trichinellosis is a foodborne disease caused by ingestion of infective Trichinella muscle larvae via pork or meat of other food animals which are susceptible to this zoonotic parasite. There are new approaches for a risk-oriented meat inspection for Trichinella in pigs which are accompanied by monitoring programmes on herd level to control freedom from this parasite. For this purpose, testing schemes utilizing serological tests with a high sensitivity and specificity are required. This study aimed at the evaluation of an ELISA and a Western Blot (WB) for the detection of anti-Trichinella-IgG in terms of sensitivity and specificity taking results of artificial digestion as gold standard. For this purpose, 144 field sera from pigs confirmed as Trichinella-free as well as 159 sera from pigs experimentally infected with T. spiralis (123), T. britovi (19) or T. pseudospiralis (17) were examined by ELISA (excretory-secretory antigen) and WB (crude worm extract). Sera from pigs experimentally infected with four other nematode species were included to investigate the cross-reactivity of the antigen used in the WB. For all Trichinella-positive pig sera, band pattern profiles were identified in the WB and results were analysed in relation to ELISA OD% values. Testing of pig sera revealed a sensitivity of 96.8% for the ELISA and 98.1% for the WB whereas the methods showed a specificity of 97.9 and 100%, respectively. WB analysis of Trichinella-positive pig sera revealed five specific band patterns of 43, 47, 61, 66, and 102 kDa of which the 43 kDa protein was identified as the predominant antigen. The frequency of the band pattern profile was irrespective of the dose and the period of infection as well as the Trichinella species investigated. In conclusion, monitoring in swine farms for Trichinella antibodies should be based on screening pig sera by means of ELISA followed by confirmatory testing through WB analysis.
Nöckler
K
K
Federal Institute for Risk Assessment, Diedersdorfer Weg 1, 12277 Berlin, Germany. karsten.noeckler@bfr.bund.de
Reckinger
S
S
Broglia
A
A
Mayer-Scholl
A
A
Bahn
P
P
D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood*; D015153:Blotting, Western / Q000379:methods / Q000592:standards / Q000662:veterinary*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000592:standards / Q000662:veterinary*; D015801:Helminth Proteins / Q000097:blood* / Q000302:isolation & purification; D008970:Molecular Weight; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013552:Swine; D013553:Swine Diseases / Q000097:blood* / Q000175:diagnosis / Q000469:parasitology; D014234:Trichinella / Q000276:immunology* / Q000302:isolation & purification; D017160:Trichinella spiralis / Q000276:immunology / Q000302:isolation & purification; D014235:Trichinellosis / Q000097:blood / Q000175:diagnosis / Q000469:parasitology / Q000662:veterinary*
D000909:Antibodies, Helminth; D015801:Helminth Proteins