Phylogenetic analysis and comparison between cow and buffalo (including Egyptian buffaloes) mitochondrial displacement-loop regions.
Mitochondrial DNA (mtDNA) analysis has been used extensively for phylogenetic analysis studies and systematics. The displacement loop (D-loop) region inside the mtDNA is a non-coding part whose analysis can indicate variations between closely related populations. This paper reports for the first time the characterization and analysis of the complete sequence of the D-loop region from Egyptian buffaloes and analysis in conjunction with previously published Indian and European Bubalus bubalis and Bos sub-tribe sequences. In the entire D-loop of the Egyptian buffaloes, we identified four haplotypes and nine polymorphic sites from the nine sequenced D-loop regions--while in the studied set of buffaloes we identified 28 polymorphic sites in the entire D-loop, and 49 polymorphic sites in the case of cows. Alignment between buffaloes and cows to evaluate the characteristics of the D-loop region showed that the second region of the conserved sequence block (CSB2) is apparently the most variable region in the D-loop between cows and buffaloes, with four insertions in all buffaloes and two substitutions, followed by the second region of the extended termination associated sequence (ETAS2) with a substitution rate of 1/10. The Egyptian buffaloes were shown to be closest to the Italian counterparts, exemplifying the closeness of ethnicity and the history of civilization of that region.
Ramadan
Hassan A I
HA
Department of Cell Biology, National Research Center, Dokki, Giza, Egypt. hameguid@yahoo.com
El-Hefnawi
Mahmoud M
MM
D000818:Animals; D001483:Base Sequence; D002020:Buffaloes / Q000145:classification* / Q000235:genetics*; D002417:Cattle / Q000145:classification* / Q000235:genetics*; D017931:DNA Primers / Q000235:genetics; D004272:DNA, Mitochondrial / Q000737:chemistry / Q000235:genetics*; D004534:Egypt; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D010802:Phylogeny; D012689:Sequence Homology, Nucleic Acid
D017931:DNA Primers; D004272:DNA, Mitochondrial