title: [Study on factors affecting mucosal permeability of gardenia extract].
abstract: OBJECTIVE
  To study the factors that affect mucosal absorption of gardenia extract.
  
  
  METHODS
  Take vitro frog skin as a model to study the vitro mucosal permeation. The impacts of the osmotic pressure and the pH value of permeation medium on the Papp of the Jasminoidin were studied, and the effect of frog skin on the stability of Jasminoidin was investigated also.
  
  
  RESULTS
  The Papp of Jasminoidin were (0.53 +/- 0.01), (0.21 +/- 0.05), (0.44 +/- 0.12), (0.42 +/- 0.13), (0.26 +/- 0.03) cm x min(-1) by using the normal saline (pH 6.88), pure water, 1.8 % NaCl solution, normal saline (pH 4.05) and normal saline (pH 10.05) as permeation medium for each. The accumulated permeation rate was (55.69 +/- 9.81)% by 12 h, using normal saline as permeation medium respectively, and there was no obvious time lag. Jasminoidin began to degrade around 8 h by affectedof frog skin, the constant of degradation rate (K) was 1.999, and the t1/2 was 0.347 h.
  
  
  CONCLUSIONS
  The mucosal permeability of gardenia extract by using the vitro model of frog skin is good, and consistent with zero level absorption process. The osmotic pressure and pH value significantly affected the permeation and the isotonic and partial neutral permeation medium are more conducive to the permeation and absorption of Jasminoidin. The degradation effect of frog skin to the Jasminoidin will not affect mucosal permeation research. In vitro model of frog skin is a suitable way to simulate mucosal permeation process of the gardenia extract.
authors.affiliation: School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 100102, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Lu
authors.forename: Shouying
authors.initials: S
authors.lastname: Du
authors.forename: Zongling
authors.initials: Z
authors.lastname: Yao
authors.forename: Yongsong
authors.initials: Y
authors.lastname: Zhai
mesh_terms: D032062:Gardenia / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D009092:Mucous Membrane / Q000187:drug effects* / Q000502:physiology; D010539:Permeability / Q000187:drug effects*; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D012867:Skin / Q000187:drug effects; D012869:Skin Absorption / Q000187:drug effects / Q000502:physiology; D012995:Solubility
chemical_list: D010936:Plant Extracts