title: Binding of anti-HLA class I antibody to endothelial cells produce an inflammatory cytokine secretory pattern.
abstract: Current methods are inadequate for the diagnosis of early chronic allograft rejection. The goal of this study was to determine whether ligation of anti-HLA antibodies to endothelial cells is associated with a distinctive cytokine secretory pattern. Human iliac artery endothelial cells (HIAEC) cultured in vitro were incubated with w6/32, an anti-HLA class I mAb. Culture supernatants collected daily for up to 4 days were tested for secretion of 13 cytokines using a multiplexed fluorescent microsphere immunoassay. Culture of HIAEC with medium containing mAb w6/32 supported the growth of HIAEC during the 4-day study period. Levels of the pro-inflammatory cytokines IL-1beta, IL-6, IL-8, and TNF-alpha became significantly increased in supernatants of HIAEC incubated with the mAb w6/32. We conclude that ligation of anti-HLA class I antibodies to HLA class I antigens in endothelial cells initiates an acute inflammatory process and detecting an inflammatory cytokine secretory pattern might be useful to diagnose sub-clinical chronic allograft rejection.
authors.affiliation: Department of Pathology, University of Utah, Salt Lake City, Utah 84112, USA.
authors.forename: Eduardo
authors.initials: E
authors.lastname: Reyes-Vargas
authors.forename: Igor Y
authors.initials: IY
authors.lastname: Pavlov
authors.forename: Thomas B
authors.initials: TB
authors.lastname: Martins
authors.forename: Jason J
authors.initials: JJ
authors.lastname: Schwartz
authors.forename: Harry R
authors.initials: HR
authors.lastname: Hill
authors.forename: Julio C
authors.initials: JC
authors.lastname: Delgado
mesh_terms: D000911:Antibodies, Monoclonal / Q000276:immunology* / Q000494:pharmacology; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D016207:Cytokines / Q000378:metabolism*; D042783:Endothelial Cells / Q000187:drug effects / Q000276:immunology* / Q000378:metabolism; D006084:Graft Rejection / Q000276:immunology; D015395:Histocompatibility Antigens Class I / Q000276:immunology*; D006801:Humans; D007083:Iliac Artery / Q000166:cytology; D007378:Interleukins / Q000378:metabolism; D014184:Transplantation, Homologous / Q000276:immunology; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D000911:Antibodies, Monoclonal; D016207:Cytokines; D015395:Histocompatibility Antigens Class I; D007378:Interleukins; D014409:Tumor Necrosis Factor-alpha