title: Asymmetric lipid bilayer formation stabilized by DNA at the air/water interface.
abstract: The lipid bis(guanidinium)-tren-cholesterol (BGTC) is a cationic cholesterol derivative bearing guanidinium polar headgroups used for gene transfection either alone or formulated as liposomes with the zwitterionic lipid 1,2-di-[cis-9-octadecenoyl]-sn-glycero-3-phosphoethanolamine (DOPE). Previous investigations have shown its ability to strongly interact with DNA and form asymmetric lipid bilayers at the air/water interface when mixed with DOPE. Here, with a view to further investigate its physicochemical behavior, we studied the interactions of mixtures of BGTC with another zwitterionic lipid, 1,2-Dimyristoyl-sn-Glycero-3-Phosphocholine, (DMPC), with DNA at the air/water interface by using the Langmuir monolayer technique coupled with Brewster Angle Microscopy (BAM) and Polarization Modulation Infra Red Reflexion Absorption (PMIRRAS) spectroscopy and we investigate DNA-BGTC/ DMPC interactions. We demonstrate that when DNA is injected into the subphase in excess compared to the positive charges of BGTC, it adsorbs to BGTC/DMPC monolayers at 20 mN/m whatever the lipid monolayer composition (1/5, 2/3 or 3/2 BGTC/DMPC molar ratio) and forms an incomplete monolayer of either isotropic or anisotropic double strands depending on the BGTC content in the monolayer. Compression beyond the collapse of some mixed DNA-BGTC/DMPC (2/3 and 3/2 molar ratio) systems leads to the formation of DNA monolayers underneath asymmetric lipid bilayers characterized by a bottom layer of BGTC in contact with DNA and a top layer mainly constituted of DMPC.
authors.affiliation: UMR CBMN, CNRS-Université Bordeaux ENITAB, IECB, Pessac, France. s.castano@cbmn.u-bordeaux.fr
authors.forename: Sabine
authors.initials: S
authors.lastname: Castano
authors.forename: Brigitte
authors.initials: B
authors.lastname: Delord
authors.forename: Annie
authors.initials: A
authors.lastname: Février
authors.forename: Jean-Marie
authors.initials: JM
authors.lastname: Lehn
authors.forename: Pierre
authors.initials: P
authors.lastname: Lehn
authors.forename: Bernard
authors.initials: B
authors.lastname: Desbat
mesh_terms: D004247:DNA / Q000737:chemistry* / Q000378:metabolism; D008051:Lipid Bilayers / Q000737:chemistry* / Q000378:metabolism; D008563:Membrane Lipids / Q000737:chemistry* / Q000378:metabolism; D008853:Microscopy / Q000379:methods; D013055:Spectrophotometry, Infrared; D013499:Surface Properties; D013816:Thermodynamics; D014867:Water / Q000737:chemistry
chemical_list: D008051:Lipid Bilayers; D008563:Membrane Lipids; D014867:Water; D004247:DNA