Rapid PFGE method for fingerprinting of Serratia marcescens isolates.
We modified the conventional PFGE procedure for Serratia marcescens to establish a rapid method. Our protocol showed modification in the bacterial lysis, washing, and restriction enzyme digestion time. This resulted in shortening the time needed by about 3 days compared to the conventional PFGE method.
Alaidan
Alwaleed
A
Department of Biological and Medical Research, King Faisal Specialist Hospital and Research Centre, Riyadh, Saudi Arabia. alaidan@kfshrc.edu.sa
Bohol
Marie Fe
MF
Al-Thawadi
Sahar I
SI
Al-Suwaine
Abdulrahman
A
Al-Qahtani
Ahmed A
AA
Al-Ahdal
Mohammed N
MN
D015373:Bacterial Typing Techniques / Q000379:methods*; D016172:DNA Fingerprinting / Q000379:methods*; D004269:DNA, Bacterial / Q000235:genetics; D016521:Electrophoresis, Gel, Pulsed-Field / Q000379:methods*; D005838:Genotype; D017720:Molecular Epidemiology / Q000379:methods; D012706:Serratia marcescens / Q000145:classification* / Q000235:genetics*; D013997:Time Factors
D004269:DNA, Bacterial