title: Identification of the gene encoding cyclin E1 (CCNE1) as a novel IGH translocation partner in t(14;19)(q32;q12) in diffuse large B-cell lymphoma.
abstract: In a subset of B-cell malignancies, the genes encoding members of the cyclin D familiy are juxtaposed to immunoglobulin loci through recurrent chromosomal translocations. Here, we identified the gene encoding cyclin E1 as novel translocation partner of the immunoglobulin heavy chain (IGH) locus involved in a t(14;19)(q32;q12) in a case of t(8;14)(q24;q32) IGH-MYC-positive leukemic diffuse large B-cell lymphoma. The translocation breakpoints were cloned and mapped to the switch region Salpha1 of IGH in 14q32 and approximately 60kb centromeric to CCNE1 in 19q12. Immunohistochemical analysis revealed overexpression of the cyclin E1 protein in this case, which to a comparable extent was observed in 3/41 independent DLBCL. These data indicate that cyclin E1 may act as a novel oncogene in B-cell lymphomagenesis.
authors.affiliation: Institute of Human Genetics, University Hospital Schleswig-Holstein, Campus Kiel, 24105 Kiel, Germany. inagel@medgen.uni-kiel.de
authors.forename: Inga
authors.initials: I
authors.lastname: Nagel
authors.forename: Takashi
authors.initials: T
authors.lastname: Akasaka
authors.forename: Wolfram
authors.initials: W
authors.lastname: Klapper
authors.forename: Stefan
authors.initials: S
authors.lastname: Gesk
authors.forename: Sebastian
authors.initials: S
authors.lastname: Böttcher
authors.forename: Matthias
authors.initials: M
authors.lastname: Ritgen
authors.forename: Lana
authors.initials: L
authors.lastname: Harder
authors.forename: Michael
authors.initials: M
authors.lastname: Kneba
authors.forename: Martin J S
authors.initials: MJ
authors.lastname: Dyer
authors.forename: Reiner
authors.initials: R
authors.lastname: Siebert
mesh_terms: D001483:Base Sequence; D002874:Chromosome Mapping; D002883:Chromosomes, Human, Pair 14 / Q000235:genetics*; D002888:Chromosomes, Human, Pair 19 / Q000235:genetics*; D019927:Cyclin E / Q000096:biosynthesis* / Q000235:genetics*; D006801:Humans; D007143:Immunoglobulin Heavy Chains / Q000235:genetics*; D016393:Lymphoma, B-Cell / Q000235:genetics*; D008228:Lymphoma, Non-Hodgkin / Q000235:genetics / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D015513:Oncogene Proteins / Q000096:biosynthesis* / Q000235:genetics*; D016271:Proto-Oncogene Proteins c-myc / Q000378:metabolism; D012689:Sequence Homology, Nucleic Acid; D014178:Translocation, Genetic
chemical_list: C475820:CCNE1 protein, human; D019927:Cyclin E; D007143:Immunoglobulin Heavy Chains; D015513:Oncogene Proteins; D016271:Proto-Oncogene Proteins c-myc