title: Protein engineering of insulin: Two novel fast-acting insulins [B16Ala]insulin and [B26Ala]insulin.
abstract: Blood glucose lowering assay proved that [B16Ala]insulin and [B26Ala]insulin exhibit potency of acute blood glucose lowering in normal pigs, which demonstrates that they are fast-acting insulin. Single-chain precursor of [B16Ala]insulin and [B26Ala]insulin is [B16Ala]PIP and [B26Ala]PIP, respectively, which are suitable for gene expression. Secretory expression level of the precursors in methylotrophic yeast Pichia pastoris was quite high, 650 mg/L and 130 mg/L, respectively. In vivo biological assay showed that the two fast-acting insulins have full or nearly full biological activity. So both [B16Ala]insulin and [B26Ala]insulin can be well developed as fast-acting insulin for clinic use.
authors.affiliation: Shanghai Institutes for Biology Sciences, Chinese Academy of Sciences, China.
authors.forename: Zhou
authors.initials: Z
authors.lastname: Zhang
authors.forename: Yuehua
authors.initials: Y
authors.lastname: Tang
authors.forename: Shiyin
authors.initials: S
authors.lastname: Yao
authors.forename: Shangquan
authors.initials: S
authors.lastname: Zhu
authors.forename: Youmin
authors.initials: Y
authors.lastname: Feng