Oxygen carrier based on hemoglobin/poly(L-lysine)-block-poly(L-phenylalanine) vesicles.
An oxygen carrier was prepared by encapsulating carbonylated hemoglobin (CO-Hb) molecules into polypeptide vesicles made from poly(L-lysine)-block-poly(L-phenylalanine) (PLL-b-PPA) diblock copolymers in aqueous medium at pH 5.8. The encapsulation was confirmed by confocal laser scanning microscopy (CLSM). The morphology and size of the vesicles were studied by field-emission scanning electron microscopy (ESEM). They had a spherical shape with a mean diameter of about 4 to 5 microm. The encapsulation efficiency of hemoglobin was 40 wt %, and the hemoglobin content in the vesicles was 32 wt %. The CO-Hb encapsulated in the PLL-b-PPA vesicles was more stable than free CO-Hb under ambient conditions. In the presence of a O(2) atmosphere, the CO-Hb in the vesicle could be converted into oxygen-binding hemoglobin (O(2)-Hb) under irradiation of visible light for 2 h. Therefore, the CO-Hb/PLL-b-PPA vesicles are expected to be used as red blood cell substitutes.
Sun
Jing
J
State Key Laboratory of Polymer Physics and Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, PR China.
Huang
Yubin
Y
Shi
Quan
Q
Chen
Xuesi
X
Jing
Xiabin
X
D001802:Blood Substitutes / Q000737:chemistry*; D002214:Capsules; D002248:Carbon Monoxide; D006454:Hemoglobins / Q000008:administration & dosage*; D006801:Humans; D010100:Oxygen; D010455:Peptides / Q000737:chemistry* / Q000627:therapeutic use; D011107:Polylysine / Q000737:chemistry
D001802:Blood Substitutes; D002214:Capsules; D006454:Hemoglobins; D010455:Peptides; D011107:Polylysine; C012001:polyphenylalanine; D002248:Carbon Monoxide; D010100:Oxygen