title: Immunization with live influenza viruses in an experimental model of allergic bronchial asthma: infection and vaccination.
abstract: BACKGROUND
  Asthmatics in particular have a need for influenza vaccines because influenza infection is a frequent cause of hospitalization of patients with bronchial asthma. Currently, only inactivated influenza vaccines are recommended for influenza prevention in asthma sufferers.
  
  
  OBJECTIVE
  The aim of our study was to analyze and compare the effects of influenza infection and vaccination with live attenuated influenza vaccine (LAIV) on different phases of experimental murine allergic bronchial asthma (acute asthma and remission phase) and on subsequent exposure to allergen in sensitized animals.
  
  
  METHODS
  Ovalbumin (OVA)-specific serum IgE levels, IL-4 production by spleen and lung lymphocytes, and histological changes in the lungs of mice infected with pathogenic virus or LAIV were studied at two phases of OVA-induced bronchial asthma (acute asthma and remission). Results Infection with pathogenic virus both in acute asthma and remission led to asthma exacerbation associated with the production of OVA-specific IgE, IL-4 and significant inflammatory infiltration in airways. Infection, even after complete virus clearance, induced the aggravation of lung inflammation and IgE production in asthmatic mice additionally exposed to OVA. Immunization with LAIV at remission did not enhance allergic inflammatory changes in the lung, OVA-specific IgE or IL-4 production. Then after additional OVA exposure, histological and immunological changes in these mice were the same as in the control group.
  
  
  CONCLUSIONS
  Influenza infection provokes asthma exacerbation regardless of the disease phase. Immunization with LAIV during the remission phase of bronchial asthma is safe and does not interfere upon subsequent contact of asthma sufferers with allergen.
authors.affiliation: Department of Virology, Institute of Experimental Medicine RAMS, Saint-Petersburg, Russia. influenza81@gmail.com
authors.forename: Tatiana
authors.initials: T
authors.lastname: Chirkova
authors.forename: Galina
authors.initials: G
authors.lastname: Petukhova
authors.forename: Daniil
authors.initials: D
authors.lastname: Korenkov
authors.forename: Anatoliy
authors.initials: A
authors.lastname: Naikhin
authors.forename: Larisa
authors.initials: L
authors.lastname: Rudenko
mesh_terms: D000818:Animals; D001249:Asthma / Q000276:immunology*; D004195:Disease Models, Animal; D005260:Female; D006801:Humans; D007073:Immunoglobulin E / Q000097:blood; D007252:Influenza Vaccines / Q000009:adverse effects* / Q000276:immunology*; D007251:Influenza, Human / Q000517:prevention & control*; D015847:Interleukin-4 / Q000378:metabolism; D008168:Lung / Q000276:immunology / Q000473:pathology; D051379:Mice; D013154:Spleen / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology; D014613:Vaccines, Attenuated / Q000009:adverse effects / Q000276:immunology
chemical_list: D007252:Influenza Vaccines; D014613:Vaccines, Attenuated; D015847:Interleukin-4; D007073:Immunoglobulin E