title: Genetic variability of the prion protein gene (PRNP) in wild ruminants from Italy and Scotland.
abstract: The genetics of the prion protein gene (PRNP) play a crucial role in determining the relative susceptibility to transmissible spongiform encephalopathies (TSEs) in several mammalian species. To determine the PRNP gene variability in European red deer (Cervus elaphus), roe deer (Capreolus capreolus) and chamois (Rupicapra rupicapra), the PRNP open reading frame from 715 samples was analysed to reveal a total of ten single nucleotide polymorphisms (SNPs). In red deer, SNPs were found in codons 15, 21, 59, 78, 79, 98, 136, 168 and 226. These polymorphisms give rise to 12 haplotypes, and one of which is identical to the PRNP of American wapiti (Rocky Mountain elk, Cervus elaphus nelsoni). One silent mutation at codon 119 was detected in chamois and no SNPs were found in roe deer. This analysis confirmed that European wild ruminants have a PRNP genetic background that is compatible with TSE susceptibility, including chronic wasting disease.
authors.affiliation: Istituto Zooprofilattico Sperimentale del Piemonte, Turin, Italy. simone.peletto@izsto.it
authors.forename: Simone
authors.initials: S
authors.lastname: Peletto
authors.forename: Matteo
authors.initials: M
authors.lastname: Perucchini
authors.forename: Cristina
authors.initials: C
authors.lastname: Acín
authors.forename: Mark P
authors.initials: MP
authors.lastname: Dalgleish
authors.forename: Hugh W
authors.initials: HW
authors.lastname: Reid
authors.forename: Roberto
authors.initials: R
authors.lastname: Rasero
authors.forename: Paola
authors.initials: P
authors.lastname: Sacchi
authors.forename: Paula
authors.initials: P
authors.lastname: Stewart
authors.forename: Maria
authors.initials: M
authors.lastname: Caramelli
authors.forename: Ezio
authors.initials: E
authors.lastname: Ferroglio
authors.forename: Elena
authors.initials: E
authors.lastname: Bozzetta
authors.forename: Daniela
authors.initials: D
authors.lastname: Meloni
authors.forename: Riccardo
authors.initials: R
authors.lastname: Orusa
authors.forename: Serena
authors.initials: S
authors.lastname: Robetto
authors.forename: Silvia
authors.initials: S
authors.lastname: Gennero
authors.forename: Wilfred
authors.initials: W
authors.lastname: Goldmann
authors.forename: Pier Luigi
authors.initials: PL
authors.lastname: Acutis
mesh_terms: D000818:Animals; D001483:Base Sequence; D004247:DNA / Q000737:chemistry / Q000235:genetics; D003670:Deer / Q000235:genetics*; D020022:Genetic Predisposition to Disease; D014644:Genetic Variation; D006239:Haplotypes; D007558:Italy; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000662:veterinary; D020641:Polymorphism, Single Nucleotide; D017096:Prion Diseases / Q000235:genetics*; D011328:Prions / Q000235:genetics*; D012606:Scotland; D017422:Sequence Analysis, DNA
chemical_list: D011328:Prions; D004247:DNA