Antimicrobial activity of calcium hydroxide pastes on Enterococcus faecalis cultivated in root canal systems.
The effectiveness of calcium hydroxide pastes: Calen and PMCC-Calen associated to chemo-mechanical preparation was assessed on Enterococcus faecalis grown within root canals. Seventy incisors were inserted into TSB medium, sterilized and contaminated with E. faecalis. Culture medium was replaced each 24 h and incubated at 37 degrees C for 72 h. After chemo-mechanical preparation, root canals were filled with Calen or PMCC-Calen (7 or 14 days). Pastes were removed and teeth were inserted into test tubes containing Enterococcosel broth. Calen paste (maintained for 7 and 14 days) induced 70% elimination of enterococci and PMCC-Calen 100% elimination only after maintenance for 14 days. These medications were significantly more effective (p<0.001) than chemo-mechanical protocol alone and PMCC-Calen maintained for 7 days, both incapable to eliminate the viability of enterococci. Calcium hydroxide pastes demonstrated important adjuvant effects in the elimination of enterococci during chemo-mechanical preparation of root canal systems. When associated with PMCC, calcium hydroxide pastes should be maintained for at least 14 days.
Lana
Patricia Elaine Panicali
PE
Department of Endodontics, Fluminense Federal University, Niterói, RJ, Brazil.
Scelza
Miriam Fatima Záccaro
MF
Silva
Licínio Esmeraldo
LE
Mattos-Guaraldi
Ana Luíza de
AL
Hirata Júnior
Raphael
R
D002126:Calcium Hydroxide / Q000494:pharmacology*; D002733:Chlorophenols / Q000494:pharmacology; D003786:Dental Pulp Cavity / Q000382:microbiology*; D004338:Drug Combinations; D013293:Enterococcus faecalis / Q000187:drug effects*; D006801:Humans; D007180:Incisor; D050296:Microbial Viability / Q000187:drug effects; D012387:Root Canal Filling Materials / Q000494:pharmacology*
D002733:Chlorophenols; D004338:Drug Combinations; D012387:Root Canal Filling Materials; C420643:calen; C029107:4-chlorophenol; D002126:Calcium Hydroxide