title: Proliferation and multi-differentiation potentials of human mesenchymal stem cells on thermoresponsive PDMS surfaces grafted with PNIPAAm.
abstract: The thermo-responsivity of PNIPAAm [poly(N-isopropylcarylamide)]-grafted PDMS [poly(dimethylsiloxane)] surface is a property that could be feasibly used for detaching cells adhered on the surface. We used benzophenone-initiated photopolymerization to graft PNIPAAm on PDMS substrates to construct the PNIPAAm-grafted PDMS surface and this PDMS surface was highly thermo-responsive. hMSCs (human mesenchymal stem cells) were used to analyse the proliferation and multi-differentiation of stem cells on the PNIPAAm-grafted PDMS surface. The results showed that hMSCs could adhere on the PNIPAAm-grafted PDMS surface at 37 degrees C and form cell colonies, and then become fibroblastic. The proliferation potential of hMSCs on the PNIPAAm-grafted PDMS surface was not significantly different from that on a plate surface coated with gelatin. However, as it proved easier to detach cells from the surface, by changing temperature, a higher viability of detached cells could be obtained with the PNIPAAm-grafted PDMS surface, using a temperature shift, compared with a gelatin-coated surface, where cells are detached by treatment with trypsin. hMSCs on the PNIPAAm-grafted PDMS surface were induced into osteoblasts, adipocytes and neurocytes under osteogenic medium, adipogenic medium and neurogenic medium respectively. The PNIPAAm-grafted PDMS surface was favourable for osteogenesis of hMSCs, although the potentials of adipogenesis and neurogenesis of hMSCs on the PNIPAAm-grafted PDMS surface were similar to those on the plate surface coated with gelatin. The above results demonstrate that the PNIPAAm-grafted PDMS surface not only kept the potentials of proliferation and multi-differentiation of hMSCs, but also increased the viability of hMSCs.
authors.affiliation: The Institute of Cell Biology and Genetics, College of Biological Sciences, Zhejiang University, Zijingang Campus, Hangzhou, Zhejiang 310058, China.
authors.forename: Dongyan
authors.initials: D
authors.lastname: Shi
authors.forename: Dan
authors.initials: D
authors.lastname: Ma
authors.forename: Feiqing
authors.initials: F
authors.lastname: Dong
authors.forename: Chen
authors.initials: C
authors.lastname: Zong
authors.forename: Liyue
authors.initials: L
authors.lastname: Liu
authors.forename: Dan
authors.initials: D
authors.lastname: Shen
authors.forename: Wenji
authors.initials: W
authors.lastname: Yuan
authors.forename: Xiangmin
authors.initials: X
authors.lastname: Tong
authors.forename: Hengwu
authors.initials: H
authors.lastname: Chen
authors.forename: Jinfu
authors.initials: J
authors.lastname: Wang
mesh_terms: D000178:Acrylamides / Q000737:chemistry* / Q000494:pharmacology; D000180:Acrylic Resins; D050156:Adipogenesis / Q000187:drug effects; D000293:Adolescent; D000328:Adult; D001577:Benzophenones / Q000737:chemistry / Q000494:pharmacology; D002448:Cell Adhesion / Q000187:drug effects; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D049109:Cell Proliferation; D002478:Cells, Cultured; D004129:Dimethylpolysiloxanes / Q000737:chemistry* / Q000494:pharmacology; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D005780:Gelatin / Q000737:chemistry / Q000494:pharmacology; D015870:Gene Expression / Q000187:drug effects; D006801:Humans; D007381:Intermediate Filament Proteins / Q000235:genetics; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D039902:Multipotent Stem Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000235:genetics; D064231:Nestin; D055495:Neurogenesis / Q000187:drug effects; D010751:Phosphopyruvate Hydratase / Q000235:genetics; D011108:Polymers / Q000737:chemistry* / Q000494:pharmacology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013499:Surface Properties; D013696:Temperature; D055815:Young Adult
chemical_list: D000178:Acrylamides; D000180:Acrylic Resins; D001577:Benzophenones; D004129:Dimethylpolysiloxanes; D007381:Intermediate Filament Proteins; C577484:NES protein, human; D009419:Nerve Tissue Proteins; D064231:Nestin; D011108:Polymers; C052970:poly-N-isopropylacrylamide; C013830:baysilon; C047723:benzophenone; D005780:Gelatin; D010751:Phosphopyruvate Hydratase