title: Covalent immobilization of Subtilisin A onto thin films of maleic anhydride copolymers.
abstract: Enzymes cleaving the biopolymer adhesives of fouling organisms are attracting attention for the prevention of biofouling. We report a versatile and robust method to confine the serine protease Subtilisin A (or Subtilisin Carlsberg) to surfaces to be protected against biofouling. The approach consists of the covalent immobilization of the protease onto maleic anhydride copolymer thin film coatings. High-swelling poly(ethylene-alt-maleic anhydride) (PEMA) copolymer layers permitted significantly higher enzyme loadings and activities than compact poly(octadecene-alt-maleic anhydride) (POMA) films. Substantial fractions of the immobilized, active enzyme layers were found to be conserved upon storage in deionized water for several hours. Ongoing studies explore the potentialities of the developed bioactive coatings to reduce the adhesion of various fouling organisms.
authors.affiliation: Leibniz Institute of Polymer Research Dresden, Max Bergmann Center of Biomaterials Dresden, Hohe Strasse 6, 01069 Dresden, Germany.
authors.forename: Mariana
authors.initials: M
authors.lastname: Tasso
authors.forename: Ana L
authors.initials: AL
authors.lastname: Cordeiro
authors.forename: Katrin
authors.initials: K
authors.lastname: Salchert
authors.forename: Carsten
authors.initials: C
authors.lastname: Werner
mesh_terms: D001672:Biocompatible Materials; D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000737:chemistry* / Q000378:metabolism; D008299:Maleic Anhydrides / Q000737:chemistry*; D008422:Materials Testing; D015394:Molecular Structure; D011108:Polymers / Q000737:chemistry*; D013381:Subtilisins / Q000737:chemistry* / Q000378:metabolism; D013499:Surface Properties; D014867:Water / Q000737:chemistry
chemical_list: D001672:Biocompatible Materials; D004800:Enzymes, Immobilized; D008299:Maleic Anhydrides; D011108:Polymers; D014867:Water; D013381:Subtilisins