title: V(D)J targeting mistakes occur at low frequency in acute lymphoblastic leukemia.
abstract: Translocations of proto-oncogenes to the B-cell or T-cell antigen receptor loci in acute T- or B-cell leukemia and lymphoma have been, in most cases, accredited to V(D)J or switch recombination depending on the location of the breakpoint at the receptor locus. Only in rare instances, the reports take into account mechanistic characteristics of the translocation mechanism. To assess the functional ability of several sites implicated in supposedly V(D)J-mediated translocations, we tested five sites at four proto-oncogene loci in an ex vivo recombination substrate assay for their potential to act as direct target for V(D)J recombination. Our results show that the LMO2/RBTN2/TTG2 site and one LCK/P56 site readily engage in recombination with a genuine TCR element with the majority of breakpoint junctions showing the characteristics of V(D)J recombination, which strongly supports the involvement of this mechanism in the pathogenesis of the corresponding translocations in vivo. The site at the TLX1/HOX11 locus yielded 0.8% V(D)J-specific junctions. Sites at the LCK/P56 and TCF3/E2A proto-oncogenes resulted in exclusively unspecific breakpoints scattered over part of or the entire proto-oncogene region tested, marking them as unlikely V(D)J recombination targets. Our data suggest that, while being a potentially dangerous mechanism due to the introduction of DNA breaks, V(D)J recombination is a tightly controlled mechanism allowing for only few direct mistakes.
authors.affiliation: Division of Hematology and Hemostaseology, Department of Medicine I, Medical University of Vienna, Vienna, Austria. katrina.vanura@meduniwien.ac.at
authors.forename: Katrina
authors.initials: K
authors.lastname: Vanura
authors.forename: Maruska Marusic
authors.initials: MM
authors.lastname: Vrsalovic
authors.forename: Trang
authors.initials: T
authors.lastname: Le
authors.forename: Rodrig
authors.initials: R
authors.lastname: Marculescu
authors.forename: Rajko
authors.initials: R
authors.lastname: Kusec
authors.forename: Ulrich
authors.initials: U
authors.lastname: Jäger
authors.forename: Bertrand
authors.initials: B
authors.lastname: Nadel
mesh_terms: D048868:Adaptor Proteins, Signal Transducing; D000818:Animals; D002478:Cells, Cultured; D053960:DNA Breaks; D004268:DNA-Binding Proteins / Q000235:genetics; D005347:Fibroblasts; D019672:Genes, T-Cell Receptor; D018398:Homeodomain Proteins / Q000235:genetics; D060588:LIM Domain Proteins; D019860:Lymphocyte Specific Protein Tyrosine Kinase p56(lck) / Q000235:genetics; D008667:Metalloproteins / Q000235:genetics; D051379:Mice; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000235:genetics*; D011519:Proto-Oncogenes / Q000235:genetics*; D011947:Receptors, Antigen, B-Cell / Q000235:genetics; D011995:Recombination, Genetic; D051820:TCF Transcription Factors / Q000235:genetics; D058125:Transcription Factor 7-Like 1 Protein; D014178:Translocation, Genetic; D045567:VDJ Recombinases / Q000235:genetics / Q000378:metabolism*
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D004268:DNA-Binding Proteins; D018398:Homeodomain Proteins; D060588:LIM Domain Proteins; C513188:Lmo2 protein, mouse; D008667:Metalloproteins; D011947:Receptors, Antigen, B-Cell; D051820:TCF Transcription Factors; C544913:Tcf7l1 protein, mouse; C487378:Tlx1 protein, mouse; D058125:Transcription Factor 7-Like 1 Protein; D019860:Lymphocyte Specific Protein Tyrosine Kinase p56(lck); D045567:VDJ Recombinases