title: Endoscopic evaluation of esophago-gastro-jejunostomy in rat model of Barrett's esophagus.
abstract: Endoscopy can be used to monitor the onset of metaplastic transformation and to observe the progression of neoplasia in small animal models of Barrett's esophagus. By avoiding animal sacrifice, the natural history of this disease can be studied in a longitudinal fashion. We aim to characterize the endoscopic features of esophageal mucosa at various stages of the metaplasia-dysplasia-carcinoma sequence in a rat reflux model of Barrett's for comparison with histology. Acid and bile reflux was produced by introducing a side-to-side esophago-gastro-jejunostomy in Sprague-Dawley rats. Endoscopic examination of the distal esophagus was performed in 24 surgically altered and 4 control rats, between weeks 24 and 36 after the operation in 4-week intervals, and all rats were biopsied and sacrificed at 36 weeks. Endoscopic images were classified based on the surface mucosal patterns of the distal esophagus and then compared with histology. The endoscopic appearance was classified as: (i) normal, characterized by a smooth surface; (ii) intestinal metaplasia, defined as elevated plaques/ridges, deep grooves, and thin linear folds; (iii) dysplasia, indicated by coarse folds/grooves, meshlike villi, and foveolar appearance; and (iv) carcinoma, suggested by irregular-shaped mass lesions with ulcerations. The endoscopic criteria for intestinal metaplasia yielded a sensitivity of 100% in comparison with histology. Intestinal metaplasia with high-grade dysplasia was found in two rats and with low-grade dysplasia in three rats. Both focally invasive squamous cell carcinoma and invasive adenocarcinoma were found in one rat. Small animal endoscopy in a rat model of Barrett's esophagus can be used to perform surveillance, classify mucosal patterns, observe the onset of intestinal metaplasia, and monitor the progression of neoplastic transformation, representing a useful tool for studying the natural history of this disease.
authors.affiliation: Division of Gastroenterology and Hepatology, Stanford University School of Medicine, Stanford, California, USA.
authors.forename: S
authors.initials: S
authors.lastname: Lu
authors.forename: A W
authors.initials: AW
authors.lastname: Lowe
authors.forename: G
authors.initials: G
authors.lastname: Triadafilopoulos
authors.forename: P-L
authors.initials: PL
authors.lastname: Hsiung
authors.forename: Y
authors.initials: Y
authors.lastname: Hao
authors.forename: J M
authors.initials: JM
authors.lastname: Crawford
authors.forename: T D
authors.initials: TD
authors.lastname: Wang
mesh_terms: D000230:Adenocarcinoma / Q000473:pathology* / Q000601:surgery; D000714:Anastomosis, Surgical; D000818:Animals; D001471:Barrett Esophagus / Q000473:pathology* / Q000601:surgery; D001707:Biopsy, Needle; D002471:Cell Transformation, Neoplastic / Q000473:pathology*; D004195:Disease Models, Animal; D004943:Esophagogastric Junction / Q000601:surgery; D004945:Esophagoscopy / Q000379:methods*; D005260:Female; D007150:Immunohistochemistry; D007582:Jejunostomy / Q000379:methods; D008297:Male; D009361:Neoplasm Invasiveness / Q000473:pathology; D011230:Precancerous Conditions / Q000473:pathology*; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D012016:Reference Values; D012680:Sensitivity and Specificity