title: On the potential of Tephrosia purpurea as anti-Helicobacter pylori agent.
abstract: OBJECTIVE
  Since Tephrosia purpurea (Linn.) Pers. (Fabaceae) has traditional use in curing different types of wounds including gastroduodenal ulcers, it was of interest to evaluate the in vitro anti-Helicobacter pylori activity profile of the plant extract and its fractions with a view to examining its therapeutic potential, if any.
  
  
  METHODS
  Employing clinical isolates and standard strains of Helicobacter pylori, the extract and fractions were bioevaluated in terms of MIC and MBC values, acid stability, time-kill kinetics, drug resistance, and synergistic potential.
  
  
  RESULTS
  The methanolic extract showed promising activity against clinical isolates and standard strains of Helicobacter pylori, including metronidazole-resistant strains. Fractionation of the extract revealed the n-hexane and chloroform fractions to possess marked activity. The extract and the less polar fractions remained functionally active in acidic condition similar to stomach environment, exhibited consistent bacteriostatic activity during repeated exposure, and demonstrated synergism, complete or partial, even with antibiotic-resistant strains.
  
  
  CONCLUSIONS
  Apolar fractions of Tephrosia purpurea may have therapeutic potential in combating Helicobacter pylori mediated gastroduodenal disorders.
authors.affiliation: Indian Institute of Chemical Biology, Kolkata 700032, India.
authors.forename: Annalakshmi
authors.initials: A
authors.lastname: Chinniah
authors.forename: Satyabrata
authors.initials: S
authors.lastname: Mohapatra
authors.forename: Suchandra
authors.initials: S
authors.lastname: Goswami
authors.forename: Anita
authors.initials: A
authors.lastname: Mahapatra
authors.forename: Sudip K
authors.initials: SK
authors.lastname: Kar
authors.forename: U V
authors.initials: UV
authors.lastname: Mallavadhani
authors.forename: Pratap K
authors.initials: PK
authors.lastname: Das
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology*; D024881:Drug Resistance, Bacterial; D004357:Drug Synergism; D005744:Gastric Acid / Q000737:chemistry; D016480:Helicobacter pylori / Q000187:drug effects* / Q000235:genetics; D008826:Microbial Sensitivity Tests; D009154:Mutation / Q000235:genetics; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology; D031304:Tephrosia / Q000737:chemistry*
chemical_list: D000900:Anti-Bacterial Agents; D010936:Plant Extracts