title: Genetic characterization of Rabies virus isolated from cattle between 1997 and 2002 in an epizootic area in the state of São Paulo, Brazil.
abstract: The biogeographical history of rabies can be reconstructed using molecular data. This work describes the genetic characterization of the Rabies virus variant that circulates in the Desmodus rotundus (vampire bat) population in an epizootic area and is transmitted to herbivorous livestock. The N and G genes of this virus were sequenced, and the phylogenetic trees generated were topologically concordant. Three genetic clusters were identified in the epizootic area and were designated RD1, RD2 and RD3. The results show that the origins of the epizootics in areas RD1 and RD2 were different and that the epizootic in area RD3 was the result of expansion of that in area RD2. The two genes analyzed are conserved, and their identities, which are greater than 98%, were maintained over time and space. The genetic sequences in this study were compared with others retrieved from GenBank, and the high identity of the N and G genes was also shown to be maintained over time and space. The results suggest that the D. rotundus lineages of the Rabies virus from the Atlantic coast of South America are highly conserved.
authors.affiliation: Pasteur Institute, Brazil. pedrocarnielijunior@ymail.com
authors.forename: Pedro
authors.initials: P
authors.lastname: Carnieli
authors.forename: Juliana Galera
authors.initials: JG
authors.lastname: Castilho
authors.forename: Willian de Oliveira
authors.initials: Wde O
authors.lastname: Fahl
authors.forename: Nazle Mendonça Collaço
authors.initials: NM
authors.lastname: Véras
authors.forename: Maria do Carmo Sampaio Tavares
authors.initials: Mdo C
authors.lastname: Timenetsky
mesh_terms: D000818:Animals; D000956:Antigens, Viral / Q000235:genetics; D001938:Brazil / Q000453:epidemiology; D002417:Cattle; D002418:Cattle Diseases / Q000453:epidemiology* / Q000821:virology*; D002685:Chiroptera / Q000821:virology; D016000:Cluster Analysis; D017124:Conserved Sequence; D004196:Disease Outbreaks; D006023:Glycoproteins / Q000235:genetics; D008969:Molecular Sequence Data; D019590:Nucleocapsid Proteins / Q000235:genetics; D010802:Phylogeny; D011110:Polymorphism, Genetic; D011818:Rabies / Q000453:epidemiology / Q000662:veterinary*; D011820:Rabies virus / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D017422:Sequence Analysis, DNA; D014759:Viral Envelope Proteins / Q000235:genetics
chemical_list: D000956:Antigens, Viral; D006023:Glycoproteins; D019590:Nucleocapsid Proteins; D014759:Viral Envelope Proteins; C064951:glycoprotein G, Rabies virus; C099601:nucleocapsid protein, Rabies virus