Remorin, a solanaceae protein resident in membrane rafts and plasmodesmata, impairs potato virus X movement.
Remorins (REMs) are proteins of unknown function specific to vascular plants. We have used imaging and biochemical approaches and in situ labeling to demonstrate that REM clusters at plasmodesmata and in approximately 70-nm membrane domains, similar to lipid rafts, in the cytosolic leaflet of the plasma membrane. From a manipulation of REM levels in transgenic tomato (Solanum lycopersicum) plants, we show that Potato virus X (PVX) movement is inversely related to REM accumulation. We show that REM can interact physically with the movement protein TRIPLE GENE BLOCK PROTEIN1 from PVX. Based on the localization of REM and its impact on virus macromolecular trafficking, we discuss the potential for lipid rafts to act as functional components in plasmodesmata and the plasma membrane.
Raffaele
Sylvain
S
Laboratoire de Biogenèse Membranaire, Unité Mixte de Recherche 5200, Centre National de la Recherche Scientifique-University of Bordeaux, Bordeaux 33076, France.
Bayer
Emmanuelle
E
Lafarge
David
D
Cluzet
Stéphanie
S
German Retana
Sylvie
S
Boubekeur
Tamy
T
Leborgne-Castel
Nathalie
N
Carde
Jean-Pierre
JP
Lherminier
Jeannine
J
Noirot
Elodie
E
Satiat-Jeunemaître
Béatrice
B
Laroche-Traineau
Jeanny
J
Moreau
Patrick
P
Ott
Thomas
T
Maule
Andrew J
AJ
Reymond
Philippe
P
Simon-Plas
Françoise
F
Farmer
Edward E
EE
Bessoule
Jean-Jacques
JJ
Mongrand
Sébastien
S
D002352:Carrier Proteins / Q000032:analysis / Q000378:metabolism / Q000502:physiology*; D002458:Cell Fractionation; D049452:Green Fluorescent Proteins / Q000032:analysis; D007113:Immunity, Innate; D018551:Solanum lycopersicum / Q000378:metabolism / Q000648:ultrastructure / Q000821:virology*; D021962:Membrane Microdomains / Q000378:metabolism*; D008969:Molecular Sequence Data; D010750:Phosphoproteins / Q000032:analysis / Q000378:metabolism / Q000502:physiology*; D010935:Plant Diseases / Q000821:virology; D010940:Plant Proteins / Q000032:analysis / Q000378:metabolism / Q000502:physiology*; D030821:Plants, Genetically Modified / Q000821:virology; D031425:Plasmodesmata / Q000378:metabolism*; D017863:Potexvirus / Q000502:physiology*; D011993:Recombinant Fusion Proteins / Q000032:analysis; D014779:Virus Replication
D002352:Carrier Proteins; D010750:Phosphoproteins; D010940:Plant Proteins; D011993:Recombinant Fusion Proteins; C103794:remorin; D049452:Green Fluorescent Proteins