Isolation and analysis of bioactive diterpenoids in Salvia species (Salvia chionantha and Salvia kronenburgiii) by micellar electrokinetic capillary chromatography.
In the present work, we isolated two bioactive diterpenoids, horminone and 7-O-acetylhorminone and developed a micellar electrokinetic chromatography (MEKC) method for the simultaneous quantitative analysis of them in Turkish Salvia species. The optimal separation electrolyte was 50 mmol/L SDS and 25% methanol at pH 11.5. The limits of detection (S/N=3) were 3.269 and 4.518 microg/mL for horminone and 7-O-acetylhorminone, respectively. The method has been applied successfully to analyze these two components in Salvia chionantha and Salvia kronenburgii acetone extracts.
Oztekin
Nevin
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Department of Chemistry, Technical University of Istanbul, Maslak, 34469 Istanbul, Turkey.
Başkan
Selda
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Evrim Kepekçi
S
S
Erim
F Bedia
FB
Topçu
Gülaçti
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D001892:Camphanes; D020374:Chromatography, Micellar Electrokinetic Capillary / Q000295:instrumentation / Q000379:methods*; D004224:Diterpenes / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification*; D004365:Drugs, Chinese Herbal / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D000432:Methanol / Q000737:chemistry; D015394:Molecular Structure; D053723:Panax notoginseng; D027544:Salvia / Q000737:chemistry* / Q000145:classification*; D027561:Salvia miltiorrhiza; D012967:Sodium Dodecyl Sulfate / Q000737:chemistry; D012997:Solvents / Q000737:chemistry; D013997:Time Factors
D001892:Camphanes; D004224:Diterpenes; D004365:Drugs, Chinese Herbal; D012997:Solvents; C000604051:danshen dripping pill; D012967:Sodium Dodecyl Sulfate; D000432:Methanol