Evaluation of a suitable DNA damage biomarker for human biomonitoring of exposed workers.
The objective of this study was to identify a sensitive and noninvasive biomarker of early genotoxic effects, for health risk assessment of workers exposed to mixtures of low doses of xenobiotics. We studied 30 workers exposed to antineoplastic drugs, 57 workers exposed to different mixtures of polycyclic aromatic hydrocarbons (PAHs) (41 airport workers and 16 paving workers) and 76 controls. Comet and micronucleus (MN) tests were performed on lymphocytes and exfoliated buccal cells. The MN assay on lymphocytes did not show significant differences between exposed and controls, while the MN assay on exfoliated buccal cells showed higher values in workers exposed to antineoplastics as compared with controls (0.85 vs. 0.48, P = 0.042). The comet assay on lymphocytes showed a higher comet percentage value (18.11 vs. 11.24 in controls, P = 0.001) and mean tail moment (TM) value (21.84 vs. 16.72 in controls, P = 0.003) in individuals exposed to PAHs as compared with controls; no significant differences were found in exposed to antineoplastics. The comet assay on exfoliated buccal cells did not show significant differences between exposed and control groups for comet percentages, whereas the TM value was higher in workers exposed to PAHs (55.1 vs. 32.31 for controls, P < 0.001). These results show that exfoliated buccal cells, obtained by a noninvasive procedure, represent robust target cells to assess the occupational exposure to inhalable mixture of chemicals at low doses. The comet assay seems to be suitable to promptly evaluate the genotoxic effects of PAHs mixtures that also contain volatile substances. The MN test is suitable to evaluate the effects of antineoplastics. Inc.
Cavallo
Delia
D
Department of Occupational Medicine, ISPESL-National Institute for Occupational Safety and Prevention, 00040 Monteporzio Catone, Rome, Italy. delia.cavallo@ispesl.it
Ursini
Cinzia Lucia
CL
Rondinone
Bruna
B
Iavicoli
Sergio
S
D000970:Antineoplastic Agents / Q000633:toxicity*; D015415:Biomarkers; D016022:Case-Control Studies; D020552:Comet Assay; D004249:DNA Damage; D004784:Environmental Monitoring / Q000379:methods*; D006801:Humans; D015162:Micronucleus Tests; D011083:Polycyclic Compounds / Q000633:toxicity*; D018570:Risk Assessment
D000970:Antineoplastic Agents; D015415:Biomarkers; D011083:Polycyclic Compounds