[Study on the relationship between microsatellite alterations of RASSF1A gene and the development of cervical carcinoma].
OBJECTIVE
To explore the relationship between microsatellite alterations of RASSF1A gene and the development of cervical carcinoma, and HPV16 infection.


METHODS
Two sites of microsatellite polymorphism of RASSF1A gene were selected, we used polymerase chain reaction (PCR) technique to detect the LOH and MSI of cervical tissues, and to detect the infection state of HPV16.


RESULTS
There were significant differences of LOH rates at the two sites between clinical stage and pathological grade (P < 0.05). Significant differences were noted between the cervical carcinomas with lymph node metastasis and those without lymph node metastasis in regard to their LOH and MSI at the two sites ( P < 0.05). The incidence of LOH of RASSF1A gene was higher in HPV16(+) than that in HPV16(-) ( P < 0.05).


CONCLUSIONS
The change of RASSF1A gene is a relatively late event in cervical carcinomas. The detection of the LOH and MSI of RASSF1A gene might be helpful to the early diagnosis and the screening of cervical carcinoma. It might also be useful for predicting the prognosis of cervical carcinoma. Infection of HPV16 and LOH of RASSF1A gene had reacted together in the development of cervical carcinoma.
Zhao
Fu-xi
FX
Institute of Microorganism and Immunology, Medical college of Datong University, Shanxi 037008, China. dtdxzxz0001@yahoo.com.cn
Yan
Jie
J
Liu
Run-hua
RH
Wang
Xi-ying
XY
Liu
Li-hua
LH
D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D019656:Loss of Heterozygosity / Q000235:genetics*; D018895:Microsatellite Repeats / Q000235:genetics*; D054778:NM23 Nucleoside Diphosphate Kinases / Q000235:genetics; D025521:Tumor Suppressor Proteins / Q000235:genetics*; D002583:Uterine Cervical Neoplasms / Q000175:diagnosis / Q000235:genetics*; D002578:Uterine Cervical Dysplasia / Q000175:diagnosis / Q000235:genetics*
D054778:NM23 Nucleoside Diphosphate Kinases; C411762:RASSF1 protein, human; D025521:Tumor Suppressor Proteins