Comprehensive allelotyping of hepatoblastoma.
OBJECTIVE
Hepatoblastoma is the most common malignant liver tumor to affect young children.


METHODS
To identify loci of potential tumor suppressor genes, associated with HB, we carried out a genome-wide allelotyping study in panel of 15 HBs using 67 highly polymorphic microsatellite markers including 14 known tumor suppressor gene loci.


RESULTS
Allelic losses at microsatellite loci were frequent on 1q (73.3%), 4q (26.7%), 13q (73.3%), 17p (46.7%), and 17q (86.7%). Deletion mapping of these chromosome arms localized commonly deleted regions to 1q44, 4q21-22, 13q14 (containing Rb1), 17p13 (containing TP53), and 17q11.2 (containing NF1).


CONCLUSIONS
Allelic losses at multiple loci may implicate chromosomal instability as an important factor in development and progression of HB.
Terada
Yoshie
Y
Department of Surgery, Nippon Medical School, 1-1-5 Sendagi, Bunkyo-ku, Tokyo 113-8603, Japan.
Matsumoto
Satoshi
S
Bando
Kouichi
K
Tajiri
Takashi
T
D000293:Adolescent; D000483:Alleles; D002648:Child; D002675:Child, Preschool; D002872:Chromosome Deletion; D002874:Chromosome Mapping; D005260:Female; D016147:Genes, Tumor Suppressor; D005838:Genotype; D018197:Hepatoblastoma / Q000235:genetics*; D006801:Humans; D007223:Infant; D008113:Liver Neoplasms / Q000235:genetics*; D019656:Loss of Heterozygosity / Q000235:genetics; D008297:Male; D018895:Microsatellite Repeats