[Some influence factors in determination of serum enzyme in hepatitis patients].
OBJECTIVE
To study some factors (temperature, time, anticoagulate, reagent, apparatus) which influence the detection of serum enzyme.


METHODS
Serums obtained from same patient are determined in different conditions. Compare the statistical difference among each group.


RESULTS
The anticoagulant-heparinize Li influence the determination of ADA and CH50. If serums were sent in room temperature for 24 hours, the results will be different. Using regents from different factories will receive different results.


CONCLUSIONS
Different conditions and reagents will influence results. To obtain correct data, we must institute and perform standard regulation.
Guo
Hong-yan
HY
Beijing You'an Hospital, Affiliated to Capital University of Medical Sciences, Beijing 100069, China.
D000243:Adenosine Deaminase / Q000378:metabolism*; D001800:Blood Specimen Collection; D015941:Complement Hemolytic Activity Assay; D003165:Complement System Proteins / Q000378:metabolism*; D004864:Equipment and Supplies; D006505:Hepatitis / Q000097:blood* / Q000201:enzymology; D006801:Humans; D007202:Indicators and Reagents; D008297:Male; D044967:Serum / Q000201:enzymology*; D013048:Specimen Handling; D013696:Temperature
D007202:Indicators and Reagents; D003165:Complement System Proteins; D000243:Adenosine Deaminase