title: Second harmonic microscopy of axonemes.
abstract: We performed Second Harmonic Microscopy of axonemes obtained from sea urchin sperm. Using polarization analysis and a trade-off between signal and photodamage, we were able to determine, for the first time to our knowledge, the nonlinear susceptibility chizxx/chixzx = 1.1+/-0.2 and chizzz/chixzx = 4+/-0.5 of axonemes.
authors.affiliation: Institut of Physics of Rennes IPR/UMR CNRS 6251, University of Rennes I, Campus de Beaulieu, Bat 11A, 35042 Rennes Cedex, France. christophe.odin@univ-rennes1.fr
authors.forename: Christophe
authors.initials: C
authors.lastname: Odin
authors.forename: Claire
authors.initials: C
authors.lastname: Heichette
authors.forename: Denis
authors.initials: D
authors.lastname: Chretien
authors.forename: Yann
authors.initials: Y
authors.lastname: Le Grand
mesh_terms: D000818:Animals; D054468:Axoneme / Q000648:ultrastructure*; D002478:Cells, Cultured; D008856:Microscopy, Fluorescence / Q000379:methods*; D008858:Microscopy, Phase-Contrast / Q000379:methods*; D012617:Sea Urchins / Q000648:ultrastructure*