title: Differences in pig muscle proteome according to HAL genotype: implications for meat quality defects.
abstract: Bidimensional electrophoresis was used to compare sarcoplasmic protein profiles of early post-mortem pig semimembranosus muscles, sampled from pigs of different HAL genotypes (RYR1 mutation 1841T/C): 6 NN, 6 Nn, 6 nn. ANOVA showed that 55 (18%) of the total of 300 matched protein spots were influenced by genotype, and hierarchical clustering analysis identified 31 (10% of the matched proteins) additional proteins coregulated with these proteins. Fold-changes of differentially expressed proteins were between 1.3 and 21.8. Peptide mass fingerprinting identification of 78 of these 86 proteins indicates that faster pH decline of nn pigs was not explained by higher abundance of glycolytic enzymes. Results indicate further that nn muscles contained fewer proteins of the oxidative metabolic pathway, fewer antioxidants, and more protein fragments. Lower abundance of small heat shock proteins and myofibrillar proteins in nn muscles may at least partly be explained by the effect of pH on their extractability. Possible consequences of lower levels of antioxidants and repair capacities, increased protein fragmentation, and lower extractability of certain proteins in nn muscles on meat quality are discussed.
authors.affiliation: INRA, UR370, Qualité des Produits Animaux, Theix, Saint-Genès-Champanelle, France. elaville@clermont.inra.fr
authors.forename: Elisabeth
authors.initials: E
authors.lastname: Laville
authors.forename: Thierry
authors.initials: T
authors.lastname: Sayd
authors.forename: Claudia
authors.initials: C
authors.lastname: Terlouw
authors.forename: Sylvie
authors.initials: S
authors.lastname: Blinet
authors.forename: Jeremy
authors.initials: J
authors.lastname: Pinguet
authors.forename: Martine
authors.initials: M
authors.lastname: Fillaut
authors.forename: Jérome
authors.initials: J
authors.lastname: Glénisson
authors.forename: Pierre
authors.initials: P
authors.lastname: Chérel
mesh_terms: D000818:Animals; D015180:Electrophoresis, Gel, Two-Dimensional; D005838:Genotype; D008297:Male; D008460:Meat / Q000032:analysis*; D008969:Molecular Sequence Data; D008970:Molecular Weight; D018482:Muscle, Skeletal / Q000737:chemistry* / Q000378:metabolism; D020543:Proteome / Q000235:genetics / Q000378:metabolism*; D011786:Quality Control; D019837:Ryanodine Receptor Calcium Release Channel / Q000235:genetics* / Q000378:metabolism; D013552:Swine / Q000235:genetics* / Q000378:metabolism*
chemical_list: D020543:Proteome; D019837:Ryanodine Receptor Calcium Release Channel