High-throughput insertional mutagenesis screens in mice to identify oncogenic networks.
Retroviral insertional mutagenesis screens have been used for many years as a tool for cancer gene discovery. In recent years, completion of the mouse genome sequence as well as improved technologies for cloning and sequencing of retroviral insertions have greatly facilitated the retrieval of more complete data sets from these screens. The concomitant increase of the size of the screens allows researchers to address new questions about the genes and signalling networks involved in tumour development. In addition, the development of new insertional mutagenesis tools such as DNA transposons enables screens for cancer genes in tissues that previously could not be analysed by retroviral insertional mutagenesis.
Kool
Jaap
J
Division of Molecular Genetics, The Cancer Genomics Centre, The Centre of Biomedical Genetics, Academic Medical Center, The Netherlands Cancer Institute, Plesmanlaan 121, Amsterdam, The Netherlands.
Berns
Anton
A
D000818:Animals; D051379:Mice; D016254:Mutagenesis, Insertional / Q000379:methods*; D009369:Neoplasms / Q000235:genetics*