title: Effect of 2-octyl-cyanoacrylate on ischemic anastomosis of the left colon.
abstract: BACKGROUND
  2-octyl-cyanoacrylate may be a good alternative in ischemic colon anastomosis without impairment of tissue perfusion.
  
  
  METHODS
  Forty male Wistar albino rats were randomized into four experimental groups (n = 10 in each group). In group 1 (control), a well-perfused left colonic segment was transected, and free ends were anastomosed by propylene sutures. In groups 2, 3, and 4, the animals underwent a standardized surgical procedure to induce ischemic left colon anastomosis. In group 2, an end-to-end anastomosis was created using six interrupted 6-0 polypropylene sutures between ischemic edges. In group 3, after approximating the mesenteric and antimesenteric edges of the anastomosis with two 6-0 polypropylene sutures, 2-octyl-cyanoacrylate was applied between the edges. In group 4, anastomosis was created by the same technique as described in group 2, and additionally 2-octyl-cyanoacrylate was applied on suture line. Rats were killed on day 4 following operation. Anastomotic integrity, intraperitoneal adhesion scores, anastomotic bursting pressures, and tissue hydroxyproline levels were recorded. Histopathological examination of the anastomosis was also performed.
  
  
  RESULTS
  There were no statistically significant differences among groups with respect to anastomotic integrity (p =.142). The mean bursting pressure values were 81.1 +/- 23.83, 43.3 +/- 26.06, 15.8 +/- 11.3, and 17.6 +/- 18.02 in groups 1, 2, 3, and 4, respectively. There were no statistically significant differences among groups 2, 3, and 4 with respect to tissue hydroxyproline levels and bursting pressure levels. The highest adhesion scores were observed in groups 3 and 4.
  
  
  CONCLUSIONS
  2-octyl cyanoacrylate does not have additional advantages in the healing of experimental ischemic colon anastomosis.
authors.affiliation: Department of General Surgery, Zonguldak Karaelmas University, Medical School Zonguldak, Turkey. oktaytip@yahoo.com
authors.forename: Oktay
authors.initials: O
authors.lastname: Irkorucu
authors.forename: Bulent Hamdi
authors.initials: BH
authors.lastname: Ucan
authors.forename: Guldeniz Karadeniz
authors.initials: GK
authors.lastname: Cakmak
authors.forename: Oge
authors.initials: O
authors.lastname: Tascilar
authors.forename: Ali Ugur
authors.initials: AU
authors.lastname: Emre
authors.forename: Ebru
authors.initials: E
authors.lastname: Ofluoglu
authors.forename: Burak
authors.initials: B
authors.lastname: Bahadir
authors.forename: Kemal
authors.initials: K
authors.lastname: Karakaya
authors.forename: Canan
authors.initials: C
authors.lastname: Demirtas
authors.forename: Hatice
authors.initials: H
authors.lastname: Pasaoglu
authors.forename: Handan
authors.initials: H
authors.lastname: Ankarali
authors.forename: Mustafa
authors.initials: M
authors.lastname: Comert
mesh_terms: D000714:Anastomosis, Surgical / Q000379:methods; D000818:Animals; D015415:Biomarkers; D003106:Colon / Q000098:blood supply* / Q000737:chemistry / Q000473:pathology / Q000601:surgery; D019245:Compressive Strength; D003487:Cyanoacrylates / Q000627:therapeutic use*; D006909:Hydroxyproline / Q000032:analysis; D007511:Ischemia / Q000601:surgery*; D008297:Male; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D013529:Surgical Wound Dehiscence / Q000517:prevention & control; D013536:Suture Techniques; D014014:Tissue Adhesives / Q000627:therapeutic use*; D014945:Wound Healing
chemical_list: D015415:Biomarkers; D003487:Cyanoacrylates; D014014:Tissue Adhesives; C100832:octyl 2-cyanoacrylate; D006909:Hydroxyproline