title: Suppression of right ventricular hypertrophy after extensive pulmonary resection in rats by granulocyte colony-stimulating factor.
abstract: BACKGROUND
  The objective of the present study was to investigate the effects of granulocyte colony-stimulating factor (G-CSF) on right ventricular hypertrophy following extensive pulmonary resection in rats.
  
  
  METHODS
  Adult rats were divided into four groups: (1) Group S (right thoracotomy only); (2) Group L (right three lobectomy); (3) Group LG10 (Group L+G-CSF [10microg/kg/d]); and (4) Group LG100 (Group L+G-CSF [100microg/kg/d]). At postoperative day 21, weight ratio of the right ventricular to the left ventricle plus septum (RV/LV+S, indicator of right ventricular hypertrophy) were measured, and a histopathological study was conducted to determine percentage wall thickness of peripheral pulmonary arteries and proliferating cell nuclear antigen labeling index (indicator of oxidative DNA damage) of right ventricles.
  
  
  RESULTS
  Mean RV/LV+S for Group S was 0.27+/-0.02, significantly smaller than that for the lobectomy groups (Group L, LG10, LG100; 0.47+/-0.05, 0.35+/-0.02, 0.38+/-0.05). G-CSF significantly suppressed right ventricular hypertrophy. Mean medial wall thickness of peripheral pulmonary arteries for Group S was 13.6% +/- 4.9%, significantly smaller than that for Group L (22.9% +/- 9.6%). Compared with Group L, G-CSF reduced medial wall thickness (LG10, 17.6% +/- 9.5%; LG100, 18.0% +/- 11.2%). Incidence of proliferating cell nuclear antigen positive nuclei for Group S was 1.07% +/- 0.49%, significantly smaller than that for Group L (13.77% +/- 5.87%). G-CSF significantly reduced the incidence of proliferating cell nuclear antigen positive nuclei (LG10, 4.04% +/- 2.14%; LG100, 3.18% +/- 1.66%).
  
  
  CONCLUSIONS
  G-CSF administration not only reduce medial wall thickness of peripheral pulmonary arteries but also directly protect cardiomyocytes of the right ventricle, thus suppressing right ventricular hypertrophy. These results suggest that low-dose G-CSF administration can prevent right heart failure following extensive pulmonary resection.
authors.affiliation: Department of General and Cardiothoracic Surgery, Graduate School of Medicine, Gifu University, Gifu, Japan. kurt@xj8.so-net.ne.jp
authors.forename: Shinsuke
authors.initials: S
authors.lastname: Matsumoto
authors.forename: Hisashi
authors.initials: H
authors.lastname: Iwata
authors.forename: Koyo
authors.initials: K
authors.lastname: Shirahashi
authors.forename: Masanao
authors.initials: M
authors.lastname: Saio
authors.forename: Yukio
authors.initials: Y
authors.lastname: Umeda
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Marui
authors.forename: Narihiro
authors.initials: N
authors.lastname: Ishida
authors.forename: Masaki
authors.initials: M
authors.lastname: Kimura
authors.forename: Takuya
authors.initials: T
authors.lastname: Sugimoto
authors.forename: Hideaki
authors.initials: H
authors.lastname: Manabe
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Takemura
mesh_terms: D000818:Animals; D038481:Anterior Temporal Lobectomy / Q000401:mortality; D016179:Granulocyte Colony-Stimulating Factor / Q000494:pharmacology* / Q000627:therapeutic use; D006454:Hemoglobins / Q000187:drug effects; D017379:Hypertrophy, Left Ventricular / Q000188:drug therapy / Q000209:etiology / Q000601:surgery; D017380:Hypertrophy, Right Ventricular / Q000188:drug therapy / Q000209:etiology / Q000601:surgery*; D007962:Leukocytes / Q000187:drug effects / Q000502:physiology; D008168:Lung / Q000254:growth & development / Q000473:pathology / Q000601:surgery*; D008297:Male; D032342:Muscle Cells / Q000502:physiology; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism; D011651:Pulmonary Artery / Q000473:pathology; D051381:Rats; D017207:Rats, Sprague-Dawley; D013908:Thoracotomy
chemical_list: D006454:Hemoglobins; D018809:Proliferating Cell Nuclear Antigen; D016179:Granulocyte Colony-Stimulating Factor