title: Control of cell migration in two and three dimensions using substrate morphology.
abstract: We have shown that en masse cell migration of fibroblasts on the planar surface results in a radial outward trajectory, and a spatially dependent velocity distribution that decreases exponentially in time towards the single cell value. If the cells are plated on the surface of aligned electrospun fibers above 1 microm in diameter, they become polarized along the fiber, expressing integrin receptors which follow closely the contours of the fibers. The velocity of the cells on the fibrous scaffold is lower than that on the planar surface, and does not depend on the degree of orientation. Cells on fiber smaller than 1 microm migrate more slowly than on the planar surface, since they appear to have a large concentration of receptors. True three-dimensional migration can be observed when plating the droplet on a scaffold comprises of at least three layers. The cells still continue to migrate on the fibers surfaces, as they diffuse into the lower layers of the fibrous scaffold.
authors.affiliation: Department of Materials Science and Engineering, Stony Brook University, NY 11794-2275, USA.
authors.forename: Ying
authors.initials: Y
authors.lastname: Liu
authors.forename: Alicia
authors.initials: A
authors.lastname: Franco
authors.forename: Lei
authors.initials: L
authors.lastname: Huang
authors.forename: Dilip
authors.initials: D
authors.lastname: Gersappe
authors.forename: Richard A F
authors.initials: RA
authors.lastname: Clark
authors.forename: Miriam H
authors.initials: MH
authors.lastname: Rafailovich
mesh_terms: D002448:Cell Adhesion / Q000502:physiology; D018929:Cell Culture Techniques / Q000295:instrumentation / Q000379:methods; D002465:Cell Movement / Q000502:physiology*; D002478:Cells, Cultured; D005347:Fibroblasts / Q000378:metabolism / Q000648:ultrastructure; D006801:Humans; D055616:Microtechnology / Q000295:instrumentation / Q000379:methods; D019904:Polymethyl Methacrylate / Q000737:chemistry; D013499:Surface Properties; D054457:Tissue Scaffolds; D016596:Vinculin / Q000378:metabolism
chemical_list: D016596:Vinculin; D019904:Polymethyl Methacrylate