title: Participation of Xenopus Elr-type proteins in vegetal mRNA localization during oogenesis.
abstract: Directional transport of specific mRNAs is of primary biological relevance. In Xenopus oocytes, mRNA localization to the vegetal pole is important for germ layer formation and germ cell development. Using a biochemical approach, we identified Xenopus Elr-type proteins, homologs of the Hu/ELAV proteins, as novel components of the vegetal mRNA localization machinery. They bind specifically to the localization elements of several different vegetally localizing Xenopus mRNAs, and they are part of one RNP together with other localization proteins, such as Vg1RBP and XStaufen 1. Blocking Elr-type protein binding by either localization element mutagenesis or antisense morpholino oligonucleotide-mediated masking of their target RNA structures, as well as overexpression of wild type and mutant ElrB proteins, interferes with vegetal localization in Xenopus oocytes.
authors.affiliation: Department of Developmental Biochemistry, Göttingen Center for Molecular Biosciences, University of Göttingen, 37077 Göttingen, Germany.
authors.forename: Patrick K
authors.initials: PK
authors.lastname: Arthur
authors.forename: Maike
authors.initials: M
authors.lastname: Claussen
authors.forename: Susanne
authors.initials: S
authors.lastname: Koch
authors.forename: Katsiaryna
authors.initials: K
authors.lastname: Tarbashevich
authors.forename: Olaf
authors.initials: O
authors.lastname: Jahn
authors.forename: Tomas
authors.initials: T
authors.lastname: Pieler
mesh_terms: D000818:Animals; D000067777:ELAV-Like Protein 2; D009865:Oocytes / Q000166:cytology* / Q000378:metabolism; D009866:Oogenesis; D017354:Point Mutation; D011485:Protein Binding; D034443:RNA Transport; D012333:RNA, Messenger / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000032:analysis / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D012261:Ribonucleoproteins / Q000032:analysis / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D014981:Xenopus / Q000378:metabolism*; D029867:Xenopus Proteins / Q000032:analysis / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*
chemical_list: D000067777:ELAV-Like Protein 2; C480172:ELAVL1 protein, Xenopus; C498635:IGF2BP3 protein, Xenopus; D012333:RNA, Messenger; D016601:RNA-Binding Proteins; D012261:Ribonucleoproteins; C488764:STAU1 protein, Xenopus; D029867:Xenopus Proteins; C000593999:elavl2 protein, Xenopus