title: Detection of a new bat gammaherpesvirus in the Philippines.
abstract: A new bat herpesvirus was detected in the spleen of an insectivorous bat (Hipposideros diadema, family Hipposideridae) collected on Panay Island, the Philippines. PCR analyses were performed using COnsensus-DEgenerate Hybrid Oligonucleotide Primers (CODEHOPs) targeting the herpesvirus DNA polymerase (DPOL) gene. Although we obtained PCR products with CODEHOPs, direct sequencing using the primers was not possible because of high degree of degeneracy. Direct sequencing technology developed in our rapid determination system of viral RNA sequences (RDV) was applied in this study, and a partial DPOL nucleotide sequence was determined. In addition, a partial gB gene nucleotide sequence was also determined using the same strategy. We connected the partial gB and DPOL sequences with long-distance PCR, and a 3741-bp nucleotide fragment, including the 3' part of the gB gene and the 5' part of the DPOL gene, was finally determined. Phylogenetic analysis showed that the sequence was novel and most similar to those of the subfamily Gammaherpesvirinae.
authors.affiliation: Department of Veterinary Microbiology, Graduate School of Agricultural and Life Sciences, University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.
authors.forename: Shumpei
authors.initials: S
authors.lastname: Watanabe
authors.forename: Naoya
authors.initials: N
authors.lastname: Ueda
authors.forename: Koichiro
authors.initials: K
authors.lastname: Iha
authors.forename: Joseph S
authors.initials: JS
authors.lastname: Masangkay
authors.forename: Hikaru
authors.initials: H
authors.lastname: Fujii
authors.forename: Phillip
authors.initials: P
authors.lastname: Alviola
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Mizutani
authors.forename: Ken
authors.initials: K
authors.lastname: Maeda
authors.forename: Daisuke
authors.initials: D
authors.lastname: Yamane
authors.forename: Azab
authors.initials: A
authors.lastname: Walid
authors.forename: Kentaro
authors.initials: K
authors.lastname: Kato
authors.forename: Shigeru
authors.initials: S
authors.lastname: Kyuwa
authors.forename: Yukinobu
authors.initials: Y
authors.lastname: Tohya
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Yoshikawa
authors.forename: Hiroomi
authors.initials: H
authors.lastname: Akashi
mesh_terms: D000818:Animals; D002685:Chiroptera / Q000821:virology*; D016000:Cluster Analysis; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics; D006564:Herpesviridae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D006566:Herpesviridae Infections / Q000662:veterinary* / Q000821:virology; D008969:Molecular Sequence Data; D010679:Philippines; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods; D017422:Sequence Analysis, DNA / Q000379:methods; D017385:Sequence Homology
chemical_list: D004279:DNA, Viral