title: Suppression of Th2-driven, allergen-induced airway inflammation by sauchinone.
abstract: Sauchinone, a lignan compound isolated from the root of Saururus chinensis, has been recently demonstrated to exhibit anti-inflammatory activity via the suppression of NF-kB p65 activity in vitro. In an effort to evaluate the in vivo anti-inflammatory function of sauchinone, we have evaluated the effects of sauchinone on allergen-induced airway inflammation using a murine model of allergic asthma. We observed that marked eosinophilic and lymphocyte infiltration in the BAL fluid were suppressed to a significant degree by sauchinone, and that mucus-secreting goblet cell hyperplasia and collagen deposition in the airways were also ameliorated by administration of sauchinone treatment. Moreover, gene expression of the inflammatory cytokines, IL-13, and IL-5 and eotaxin in the lung, and IL-5 in the draining lymph node were significantly decreased in sauchinone-treated mice. We demonstrated that sauchinone repressed Th2 cell development in vitro and IL-4 production by Th2 cells, and also inhibited GATA-3-mediated IL-5 promoter activity in a dose-dependent manner. Collectively, sauchinone ameliorated allergen-induced airway inflammation, in part, by repressing GATA-3 activity for Th2 cell development, indicating the possible therapeutic potential of sauchinone in airway inflammatory diseases including allergic asthma and rhinitis.
authors.affiliation: College of Pharmacy and Division of Life and Pharmaceutical Sciences and Center for Cell Signaling & Drug Discovery Research, Ewha Womans University, Seoul, Republic of Korea.
authors.forename: Hyun Jung
authors.initials: HJ
authors.lastname: Min
authors.forename: Hee Yeon
authors.initials: HY
authors.lastname: Won
authors.forename: Young Choong
authors.initials: YC
authors.lastname: Kim
authors.forename: Sang Hyun
authors.initials: SH
authors.lastname: Sung
authors.forename: Mi Ran
authors.initials: MR
authors.lastname: Byun
authors.forename: Jun-Ha
authors.initials: JH
authors.lastname: Hwang
authors.forename: Jeong-Ho
authors.initials: JH
authors.lastname: Hong
authors.forename: Eun Sook
authors.initials: ES
authors.lastname: Hwang
mesh_terms: D000485:Allergens; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000627:therapeutic use*; D001249:Asthma / Q000188:drug therapy* / Q000276:immunology; D001578:Benzopyrans / Q000627:therapeutic use*; D054413:Chemokine CCL11 / Q000235:genetics; D004149:Dioxoles / Q000627:therapeutic use*; D050990:GATA3 Transcription Factor / Q000378:metabolism; D015870:Gene Expression / Q000187:drug effects; D020397:Goblet Cells / Q000187:drug effects / Q000378:metabolism; D007165:Immunosuppression Therapy; D018793:Interleukin-13 / Q000235:genetics; D019409:Interleukin-15 / Q000235:genetics; D015847:Interleukin-4 / Q000235:genetics; D015848:Interleukin-5 / Q000235:genetics; D008198:Lymph Nodes / Q000187:drug effects / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D011401:Promoter Regions, Genetic / Q000187:drug effects; D012221:Rhinitis, Allergic, Perennial / Q000188:drug therapy* / Q000276:immunology; D018418:Th2 Cells / Q000187:drug effects* / Q000276:immunology
chemical_list: D000485:Allergens; D000893:Anti-Inflammatory Agents; D001578:Benzopyrans; C515457:Ccl11 protein, mouse; D054413:Chemokine CCL11; D004149:Dioxoles; D050990:GATA3 Transcription Factor; C494748:Gata3 protein, mouse; D018793:Interleukin-13; D019409:Interleukin-15; D015848:Interleukin-5; C410958:sauchinone; D015847:Interleukin-4