[Use of mathematical model for determination of virus neutralizing activity of antibodies during experimental SHIV-infection].
OBJECTIVE
To study the role of neutralizing antibodies (NA) on the model of SHIV-infection in pig-tailed macaques immunized by different methods.


METHODS
Titers of NA were determined using TZM-bl cells; titers corresponding to 50% neutralization were determined from serum dilution--relative luminescence graph. We have proposed to use index of integral neutralizing activity, which mathematically expressed as integral of neutralization function in serum dilutions interval from 1 (or first dilution used) to that giving selected titer (we selected 50% neutralization).


RESULTS
On the basis of analysis of data on course of SHIV-infection in previously immunized macaques it was concluded that more clear correlation exists between results of infection and integral neutralizing activity of serum compared with NA titers corresponding to 50% neutralization.


CONCLUSIONS
Proposed method could be useful for analysis of immunization efficacy against HIV infection.
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D000818:Animals; D000914:Antibodies, Viral / Q000097:blood / Q000276:immunology*; D002460:Cell Line; D007114:Immunization; D008251:Macaca; D008432:Mathematical Computing; D018448:Models, Immunological; D009500:Neutralization Tests; D016865:Reassortant Viruses / Q000276:immunology*; D016097:Simian Acquired Immunodeficiency Syndrome / Q000097:blood / Q000276:immunology*; D015302:Simian Immunodeficiency Virus / Q000235:genetics / Q000276:immunology*
D000914:Antibodies, Viral