Gallic acid inhibits murine leukemia WEHI-3 cells in vivo and promotes macrophage phagocytosis.
Gallic acid is a polyhydroxyphenolic compound which can be found in various natural products. It is recognized to be an excellent free radical scavenger and has been shown to induce apoptosis in lung cancer and leukemia cells. No report has addressed whether gallic acid affects mouse leukemia cells in vivo. In this study, we examined the in vivo effects of gallic acid on leukemia WEHI-3 cells and on macrophage phagocytosis. Gallic acid caused a significant decrease in the weights of the spleens and livers from BALB/c mice. One of the major characteristic of WEHI-3 leukemia is the enlarged spleen in mice after i.p. injection of WEHI-3 cells. Gallic acid did not affect the percentages of CD3, CD11 and CD19 markers but decreased the percentage of Mac-3 in a high-dose (80 mg/kg) treatment while promoting Mac-3 levels in a low-dose (40 mg/kg) treatment. Gallic acid promoted the activity of macrophage phagocytosis in the white blood cells from peripheral blood mononuclear cells (PBMCs) at 40 and 80 mg/kg treatment doses, but decreased the macrophage phagocytosis in isolated peritoneal cells at the 80 mg/kg dose.
Ho
Chin-Chin
CC
Department of Nursing, Central Taiwan University of Science and Technology, Taichung, Taiwan.
Lin
Shuw-Yuan
SY
Yang
Jai-Sing
JS
Liu
Kuo-Ching
KC
Tang
Yih-Jing
YJ
Yang
Mei-Due
MD
Chiang
Jo-Hua
JH
Lu
Chi-Cheng
CC
Wu
Chang-Lin
CL
Chiu
Tsan-Hung
TH
Chung
Jing-Gung
JG
D000818:Animals; D001835:Body Weight / Q000187:drug effects; D045744:Cell Line, Tumor; D005707:Gallic Acid / Q000494:pharmacology*; D007942:Leukemia, Experimental / Q000473:pathology*; D008264:Macrophages / Q000187:drug effects*; D051379:Mice; D008807:Mice, Inbred BALB C; D009929:Organ Size / Q000187:drug effects; D010587:Phagocytosis / Q000187:drug effects*
D005707:Gallic Acid