Delayed P2X4R expression after hypoxia-ischemia is associated with microglia in the immature rat brain.
In a preterm hypoxia-ischemia model in the post-natal day 3 rat, we characterized how the expression of purine ionotropic P2X(4) receptors change in the brain post-insult. After hypoxia-ischemia, P2X(4) receptor expression increased significantly and was associated with a late increase in ionised calcium binding adapter molecule-1 protein expression indicative of microglia cell activation. Minocycline, a potent inhibitor of microglia, attenuated the hypoxia-ischemia-induced increase in P2X(4) receptor expression. We postulate that P2X(4) receptor-positive microglia may represent a population of secondary injury-induced activated microglia. Future studies will determine whether this population contributes to the progression of injury in the immature brain.
Wixey
Julie A
JA
Perinatal Research, Clinical Neuroscience, University of Queensland Centre for Clinical Research, Herston, Queensland 4029, Australia.
Reinebrant
Hanna E
HE
Carty
Michelle L
ML
Buller
Kathryn M
KM
D000818:Animals; D001923:Brain Chemistry; D002135:Calcium-Binding Proteins / Q000032:analysis; D003337:Corpus Callosum / Q000737:chemistry; D005260:Female; D020925:Hypoxia-Ischemia, Brain / Q000378:metabolism*; D007150:Immunohistochemistry; D008297:Male; D008840:Microfilament Proteins; D017628:Microglia / Q000737:chemistry / Q000502:physiology*; D008911:Minocycline / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D018048:Receptors, Purinergic P2 / Q000032:analysis*; D058848:Receptors, Purinergic P2X4
C100745:Aif1 protein, rat; D002135:Calcium-Binding Proteins; D008840:Microfilament Proteins; C548228:P2rx4 protein, rat; D018048:Receptors, Purinergic P2; D058848:Receptors, Purinergic P2X4; D008911:Minocycline