[Study on inhibitive effect of extracts from Scindapsus aureus on alpha-glucosidase and its antioxidant activity].
OBJECTIVE
To study the inhibitive effect of extracts from Scindapsus aureus on alpha-glucosidase and its antioxidant activity.


METHODS
The 70% ethanol extracts was extracted by petroleum, ethyl acetate and n-butanol, and the inhibitory activity against alpha-glucosidases and antioxidative effects of each fraction were determined in vitro, and the inhibitory kinetics of ethyl acetate fraction was investigated.


RESULTS
The inhibitory activity of ethly acetate fraction against alpha-glucosidase was higher as its IC50 was 43.63 microg/mL. The inhibition kinetics analyzed by Hnewaver-Burk plots showed that ethyl acetate fraction was a competitive type inhibitor, and the inhibition constants for free ennzyme (Ki) was determined to be 21.49 microg/mL. The antioxidative effects of ethly acetate and n-butanol fractions exhibited stronger than that of other fractions, the scavenging ability on DPPH at the concentration of 0.42 mg/mL were 88.5% and 93.6%. Ethly acetate fraction showed scavenging ability on autioxidantor, and the IC50 values was 0.27 mg/mL.


CONCLUSIONS
Ethyld acetate fraction of extract from Scindapsus aureus has potential inhibitroy activity against alpha-glucosidase and antioxidant effects, and n-butanol fraction has great antioxidative effects.
Zhu
Qin
Q
Faculty of Chemical Engineering and Light Industry, Guangdong University of Technology, Gunangzhou 510006, China.
Zhang
Kun
K
Du
Zhi-yun
ZY
Xu
Xue-tao
XT
Jiang
Yong-fu
YF
Yu
Xin-hua
XH
D000085:Acetates; D000975:Antioxidants / Q000302:isolation & purification / Q000494:pharmacology*; D029064:Araceae / Q000737:chemistry* / Q000254:growth & development; D000431:Ethanol; D005609:Free Radicals / Q000378:metabolism*; D065089:Glycoside Hydrolase Inhibitors; D020128:Inhibitory Concentration 50; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology*; D010946:Plants, Medicinal / Q000737:chemistry / Q000254:growth & development; D013056:Spectrophotometry, Ultraviolet; D000520:alpha-Glucosidases / Q000378:metabolism
D000085:Acetates; D000975:Antioxidants; D005609:Free Radicals; D065089:Glycoside Hydrolase Inhibitors; D010936:Plant Extracts; D000431:Ethanol; C007650:ethyl acetate; D000520:alpha-Glucosidases