title: Impact of lifelong sedentary behavior on mitochondrial function of mice skeletal muscle.
abstract: This study investigated the impact of lifelong sedentariness on skeletal muscle mass and mitochondrial function. Thirty C57BL/6 strain mice (2 months) were randomly divided into three groups (young-Y; old sedentary-OS; old active-OA). Young animals were sacrificed after 1 week of quarantine, and OS and OA groups were individually placed into standard cages and in cages with running wheels, respectively, until sacrifice (25 months). Body weights and hind-limb skeletal muscle wet weights were obtained from all groups. Mitochondrial respiratory functional measures (i.e., state 3 and 4 respiration, respiratory control ratio, and ratio of nanomoles of ADP phosphorylated by nanomoles of O2 consumed [ADP/O]) and biochemical markers of oxidative damage (aconitase activity, protein carbonyl derivatives, sulfhydryl groups) were measured in isolated mitochondrial suspensions. Our results reveal that lifelong sedentary behavior has a negative impact on the age-related loss of skeletal muscle mass and on the isolated mitochondrial function of mixed skeletal muscle of mice, which is associated with an increased oxidative damage to mitochondrial biomolecules.
authors.affiliation: Centro de Investigação em Actividade Física, Saúde e Lazer, Faculty of Sports, University of Porto, Rua Dr. Plácido Costa, 91, 4200-450 Porto, Portugal. pfigueiredo@ismai.pt
authors.forename: Pedro A
authors.initials: PA
authors.lastname: Figueiredo
authors.forename: Scott K
authors.initials: SK
authors.lastname: Powers
authors.forename: Rita M
authors.initials: RM
authors.lastname: Ferreira
authors.forename: Francisco
authors.initials: F
authors.lastname: Amado
authors.forename: Hans J
authors.initials: HJ
authors.lastname: Appell
authors.forename: José A
authors.initials: JA
authors.lastname: Duarte
mesh_terms: D000154:Aconitate Hydratase / Q000378:metabolism; D000244:Adenosine Diphosphate / Q000494:pharmacology; D000375:Aging / Q000502:physiology*; D000818:Animals; D002950:Citrate (si)-Synthase / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D008931:Mitochondria, Muscle / Q000502:physiology*; D018482:Muscle, Skeletal / Q000502:physiology*; D018384:Oxidative Stress; D010101:Oxygen Consumption; D010766:Phosphorylation; D010805:Physical Conditioning, Animal
chemical_list: D000244:Adenosine Diphosphate; D002950:Citrate (si)-Synthase; D000154:Aconitate Hydratase