title: Standardization of the egg hatch test for the detection of benzimidazole resistance in parasitic nematodes.
abstract: The ability to reliably detect anthelmintic resistance is a crucial part of resistance management. If data between countries are to be compared, the same test should give the same results in each laboratory. As the egg hatch test for benzimidazole resistance is used for both research and surveys, the ability of different laboratories to obtain similar results was studied through testing of known isolates of cyathostomins, Haemonchus contortus, Ostertagia ostertagi, and Cooperia oncophora in programs supported by the EU (Cost B16 and FP6-PARASOL). Initial results showed difficulties in obtaining reproducible and similar data within and between laboratories. A series of ring tests, i.e., simultaneous and coordinated rounds of testing of nematode isolates in different laboratories was subsequently performed. By adopting identical protocols, especially the use of deionized water and making dilutions of thiabendazole in dimethyl sulfoxide in the final ring test, laboratories correctly identified both susceptible and resistant isolates. The protocols for the test and preparation of solutions of thiabendazole are described.
authors.affiliation: Institute for Parasitology, University of Veterinary Medicine, Hannover, Germany. gvsamson@tiho-hannover.de
authors.forename: Georg
authors.initials: G
authors.lastname: von Samson-Himmelstjerna
authors.forename: Gerald C
authors.initials: GC
authors.lastname: Coles
authors.forename: Frank
authors.initials: F
authors.lastname: Jackson
authors.forename: Christian
authors.initials: C
authors.lastname: Bauer
authors.forename: Fred
authors.initials: F
authors.lastname: Borgsteede
authors.forename: Veli Y
authors.initials: VY
authors.lastname: Cirak
authors.forename: Janina
authors.initials: J
authors.lastname: Demeler
authors.forename: Alison
authors.initials: A
authors.lastname: Donnan
authors.forename: Pierre
authors.initials: P
authors.lastname: Dorny
authors.forename: Christian
authors.initials: C
authors.lastname: Epe
authors.forename: Achim
authors.initials: A
authors.lastname: Harder
authors.forename: Johan
authors.initials: J
authors.lastname: Höglund
authors.forename: Ronald
authors.initials: R
authors.lastname: Kaminsky
authors.forename: Dominique
authors.initials: D
authors.lastname: Kerboeuf
authors.forename: Ulla
authors.initials: U
authors.lastname: Küttler
authors.forename: Elias
authors.initials: E
authors.lastname: Papadopoulos
authors.forename: Janez
authors.initials: J
authors.lastname: Posedi
authors.forename: John
authors.initials: J
authors.lastname: Small
authors.forename: Marián
authors.initials: M
authors.lastname: Várady
authors.forename: Jozef
authors.initials: J
authors.lastname: Vercruysse
authors.forename: Nicole
authors.initials: N
authors.lastname: Wirtherle
mesh_terms: D000818:Animals; D000871:Anthelmintics / Q000494:pharmacology*; D001562:Benzimidazoles / Q000494:pharmacology*; D004351:Drug Resistance; D004531:Eggs; D006189:Haemonchus / Q000187:drug effects; D009348:Nematoda / Q000187:drug effects*; D010028:Ostertagia / Q000187:drug effects; D021261:Parasitic Sensitivity Tests / Q000379:methods* / Q000592:standards*; D015203:Reproducibility of Results; D014251:Trichostrongyloidea / Q000187:drug effects
chemical_list: D000871:Anthelmintics; D001562:Benzimidazoles; C031000:benzimidazole