pfaB products determine the molecular species produced in bacterial polyunsaturated fatty acid biosynthesis.
When pDHA4, a vector carrying all five pfaA-pfaE genes responsible for docosahexaenoic acid (DHA; 22:6) biosynthesis in Moritella marina MP-1, was coexpressed in Escherichia coli with the individual pfaA-pfaD genes for eicosapentaenoic acid (EPA; 20:5) biosynthesis from Shewanella pneumatophori SCRC-2738, both polyunsaturated fatty acids were synthesized only in the recombinant carrying pfaB for EPA synthesis. Escherichia coli coexpressing a deleted construct comprising pfaA, pfaC, pfaD and pfaE for EPA and pfaB for DHA produced EPA and DHA. Both EPA and DHA were detected in bacteria that inherently contained pfa genes for DHA. These results suggest that PfaB is the key enzyme determining the final product in EPA or DHA biosynthesis.
Orikasa
Yoshitake
Y
Laboratory of Environmental Molecular Biology, Faculty of Environmental Earth Science, Hokkaido University, Sapporo, Japan.
Tanaka
Mika
M
Sugihara
Shinji
S
Hori
Ryuji
R
Nishida
Takanori
T
Ueno
Akio
A
Morita
Naoki
N
Yano
Yutaka
Y
Yamamoto
Kouhei
K
Shibahara
Akira
A
Hayashi
Hidenori
H
Yamada
Yohko
Y
Yamada
Akiko
A
Yu
Reiko
R
Watanabe
Kazuo
K
Okuyama
Hidetoshi
H
D001426:Bacterial Proteins / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D004281:Docosahexaenoic Acids / Q000737:chemistry* / Q000378:metabolism; D015118:Eicosapentaenoic Acid / Q000096:biosynthesis / Q000737:chemistry*; D005231:Fatty Acids, Unsaturated / Q000096:biosynthesis* / Q000737:chemistry; D005786:Gene Expression Regulation; D015964:Gene Expression Regulation, Bacterial; D034221:Moritella / Q000235:genetics / Q000378:metabolism*; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism
D001426:Bacterial Proteins; D005231:Fatty Acids, Unsaturated; D011994:Recombinant Proteins; D004281:Docosahexaenoic Acids; D015118:Eicosapentaenoic Acid