title: Peptic and tryptic digestion of peptides and proteins monitored by capillary electrophoresis with contactless conductivity detection.
abstract: The feasibility of monitoring the peptic and tryptic digestion of peptides and proteins with capillary electrophoresis using contactless conductivity detection was investigated. The peptide minigastrin I and the proteins cytochrome c from bovine heart, human serum albumin (HSA), myoglobin, and bovine serum albumin (BSA) were digested off-line with pepsin, and the resulting peptide and amino acid fragments were successfully separated and detected by conductivity measurement. Cytochrome c and myoglobin were also subjected to off-line cleavage with trypsin. On-line digestion using the electrophoretically mediated microanalysis (EMMA) approach was demonstrated with cytochrome c and apomyoglobin using trypsin.
authors.affiliation: Department of Chemistry, University of Basel, 4056 Basel, Switzerland.
authors.forename: Aiping
authors.initials: A
authors.lastname: Schuchert-Shi
authors.forename: Peter C
authors.initials: PC
authors.lastname: Hauser
mesh_terms: D004553:Electric Conductivity; D019075:Electrophoresis, Capillary / Q000379:methods*; D010455:Peptides / Q000032:analysis*; D011506:Proteins / Q000032:analysis*; D014357:Trypsin / Q000494:pharmacology
chemical_list: D010455:Peptides; D011506:Proteins; D014357:Trypsin