title: Induction of gene pattern changes associated with dysfunctional lipid metabolism induced by dietary fat and exposure to a persistent organic pollutant.
abstract: Environmental modulators of chronic diseases can include nutrition, lifestyle, as well as exposure to environmental toxicants such as persistent organic pollutants. A study was designed to explore gene expression changes as affected by both dietary fat and exposure to the polychlorinated biphenyl PCB77. Mice were fed for 4 months diets enriched with high-linoleic acid oils (20% and 40% as calories), and during the last 2 months half of each group was exposed to PCB77. Ribonucleic acids (RNA) were extracted from liver tissue to determine gene expression changes using DNA microarray analysis. Our microarray data demonstrated a significant interaction between dietary fat and PCB exposure. Deregulated genes were organized into patterns describing the interaction of diet and PCB exposure. Annotation of the deregulated genes matching these probe sets revealed a significant high-fat mediated induction of genes associated with fatty acid metabolism, triacylglycerol synthesis and cholesterol catabolism, which was down-regulated in animals exposed to PCB77. Many of these genes are regulated by the peroxisome proliferator activated receptor-alpha (PPARalpha), and changes in PPARalpha gene expression followed the same gene pattern as described above. These results provide insight into molecular mechanisms of how dietary fat can interact with environmental pollutants to compromise lipid metabolism.
authors.affiliation: Molecular and Cell Nutrition Laboratory, Department of Animal and Food Sciences, University of Kentucky, Lexington, KY 40536, USA.
authors.forename: Xabier
authors.initials: X
authors.lastname: Arzuaga
authors.forename: Na
authors.initials: N
authors.lastname: Ren
authors.forename: Arnold
authors.initials: A
authors.lastname: Stromberg
authors.forename: Esther P
authors.initials: EP
authors.lastname: Black
authors.forename: Violeta
authors.initials: V
authors.lastname: Arsenescu
authors.forename: Lisa A
authors.initials: LA
authors.lastname: Cassis
authors.forename: Zuzana
authors.initials: Z
authors.lastname: Majkova
authors.forename: Michal
authors.initials: M
authors.lastname: Toborek
authors.forename: Bernhard
authors.initials: B
authors.lastname: Hennig
mesh_terms: D000818:Animals; D001835:Body Weight; D004041:Dietary Fats / Q000494:pharmacology*; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000187:drug effects*; D050356:Lipid Metabolism / Q000187:drug effects*; D008099:Liver / Q000033:anatomy & histology / Q000187:drug effects; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009929:Organ Size; D011078:Polychlorinated Biphenyls / Q000633:toxicity*; D040081:Protein Array Analysis
chemical_list: D004041:Dietary Fats; D011078:Polychlorinated Biphenyls; C028451:3,4,3',4'-tetrachlorobiphenyl