title: [Study on the fingerprint of processed Rhizoma Atractylodis Macrocephalae by HPLC].
abstract: OBJECTIVE
  To establish HPLC fingerprint for the quality control of processed Rhizoma Atractylodis Macrocephalae (PRAM).
  
  
  METHODS
  14 batches of PRAM were collected from different places and were analyzed with the developed HPLC fingerprints method. The HPLC separation was performed on a Kromasil C18 analytical column (250 mm x 4.6 mm, 5 microm), and gradient elution was performed by mobile phase containing acetonitrile and water. The flow rate was 1.0 mL/min and the detection wavelength was at 242 nm. The temperature of column was 25 degrees C.
  
  
  RESULTS
  Sixteen mutual peaks were selected in chromatography. Among the obtained fingerprints, the most of the detected peaks were separated effectively. The methodological evaluation showed that the method had a good repeatability.
  
  
  CONCLUSIONS
  The RSD of relative retention time of mutual peaks which existed in all samples was less than 1.1%. The results of peak areas were in accordance with the request of fingerprint. The established fingerprint can be used for the quality control and species identifying of PRAM.
authors.affiliation: Department of Pharmacy, Affiliated Dongfeng Hospital, Yunyang Medical College, Shiyan 442008, China. cqh77@163.com
authors.forename: Qin-hua
authors.initials: QH
authors.lastname: Chen
authors.forename: Peng
authors.initials: P
authors.lastname: Li
authors.forename: Jing
authors.initials: J
authors.lastname: He
authors.forename: Jun
authors.initials: J
authors.lastname: Zhu
authors.forename: Shu-yan
authors.initials: SY
authors.lastname: Chen
mesh_terms: D019659:Asteraceae / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D011786:Quality Control; D015203:Reproducibility of Results; D027343:Rhizome / Q000737:chemistry
chemical_list: D004365:Drugs, Chinese Herbal