Angiotensin II induces type I collagen gene expression in human dermal fibroblasts through an AP-1/TGF-beta1-dependent pathway.
Angiotensin II is critically involved in skin wound healing, but the underlying mechanism remains unclear. This study investigated the effect of angiotensin II on type I collagen gene activation in human dermal fibroblasts and the possible mechanism involved. Angiotensin II stimulated the mRNA and protein expression of type I collagen and TGF-beta1. Effects were abolished by the angiotensin AT1 receptor antagonist ZD7155 but not by the AT2 blocker PD123319. Blockade of TGF-beta1 markedly inhibited angiotensin II-induced type I collagen gene expression. Activator protein-1 (AP-1) decoy ODNs transfection suppressed angiotensin II-induced TGF-beta1 expression, and also, diminished type I collagen expression. These data indicated that angiotensin II induces collagen gene activation in human dermal fibroblasts through an AT1-mediated AP-1/TGF-beta1 signaling pathway.
Tang
Hong-Tai
HT
Chinese PLA Institute of Burn Surgery & Burn Center, Changhai Hospital, Second Military Medical University, Shanghai 200433, China.
Cheng
Da-Sheng
DS
Jia
Yi-Tao
YT
Ben
Dao-Feng
DF
Ma
Bing
B
Lv
Kai-Yang
KY
Wei
Duo
D
Sheng
Zhi-Yong
ZY
Xia
Zhao-Fan
ZF
D000804:Angiotensin II / Q000494:pharmacology / Q000502:physiology*; D002478:Cells, Cultured; D024042:Collagen Type I / Q000235:genetics*; D000090602:Collagen Type I, alpha 1 Chain; D005347:Fibroblasts / Q000378:metabolism; D015870:Gene Expression; D005786:Gene Expression Regulation; D006801:Humans; D012867:Skin / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D018808:Transcription Factor AP-1 / Q000235:genetics / Q000378:metabolism*; D053773:Transforming Growth Factor beta1 / Q000235:genetics / Q000378:metabolism*; D014945:Wound Healing / Q000235:genetics
D024042:Collagen Type I; D000090602:Collagen Type I, alpha 1 Chain; D018808:Transcription Factor AP-1; D053773:Transforming Growth Factor beta1; D000804:Angiotensin II