Oral vaccination of mice against Helicobacter pylori with recombinant Lactococcus lactis expressing urease subunit B.
To determine whether a protective immune response could be elicited by oral delivery of a recombinant live bacterial vaccine, Helicobacter pylori urease subunit B (UreB) was expressed for extracellular expression in food-grade bacterium Lactococcus lactis. The UreB-producing strains were then administered orally to mice, and the immune response to UreB was examined. Orally vaccinated mice produced a significant UreB-specific serum immunoglobulin G (IgG) response. Specific anti-UreB IgA responses could be detected in the feces of mice immunized with the secreting lactococcal strain. Mice vaccinated orally were significantly protected against gastric Helicobacter infection following a challenge with H. pylori strain SS1. In conclusion, mucosal vaccination with L. lactis expressing UreB produced serum IgG and UreB-specific fecal IgA, and prevented gastric infection with H. pylori.
Gu
Qing
Q
Department of Biotechnology, Zhejiang Gongshang University, Hangzhou 310035, China. guqing2002@hotmail.com
Song
Dafeng
D
Zhu
Muyuan
M
D000284:Administration, Oral; D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D001426:Bacterial Proteins / Q000235:genetics / Q000276:immunology*; D001428:Bacterial Vaccines / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*; D015169:Colony Count, Microbial; D005243:Feces / Q000737:chemistry; D015870:Gene Expression; D005822:Genetic Vectors; D016481:Helicobacter Infections / Q000517:prevention & control*; D016480:Helicobacter pylori / Q000235:genetics / Q000276:immunology*; D007070:Immunoglobulin A / Q000032:analysis; D007074:Immunoglobulin G / Q000097:blood; D013294:Lactococcus lactis / Q000235:genetics*; D051379:Mice; D008807:Mice, Inbred BALB C; D021122:Protein Subunits / Q000235:genetics / Q000276:immunology; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology; D013270:Stomach / Q000382:microbiology; D014510:Urease / Q000235:genetics / Q000276:immunology*
D000907:Antibodies, Bacterial; D001426:Bacterial Proteins; D001428:Bacterial Vaccines; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D021122:Protein Subunits; D011994:Recombinant Proteins; D014510:Urease