Development of a pUC19-based recombinant plasmid to serve as a positive control in PCR for Orientia tsutsugamushi.
A recombinant positive control plasmid was developed to be used in PCR for Orientia tsutsugamushi. This pUC19-based plasmid contains the O. tsutsugamushi DNA sequence, part of which is replaced with Candida albicans DNA. pUC19-Posi is a PCR control that provides several advantages over currently used positive controls for both universal and serotypical PCR.
Hanaoka
Nozomu
N
Department of Virology-I, National Institute of Infectious Diseases, Shinjuku-ku, Tokyo 162-8640, Japan.
Sakata
Akiko
A
Takano
Ai
A
Kawabata
Hiroki
H
Watanabe
Haruo
H
Kurane
Ichiro
I
Kishimoto
Toshio
T
Ando
Shuji
S
D002176:Candida albicans / Q000235:genetics; D004274:DNA, Recombinant / Q000235:genetics*; D012285:Orientia tsutsugamushi / Q000235:genetics*; D010957:Plasmids / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods / Q000592:standards*; D012015:Reference Standards
D004274:DNA, Recombinant