Effect of NH4+ and glycerol on cytidine 5'-diphosphocholine synthesis in Saccharomyces cerevisiae.
Both stimulation of ammonium ion on the glycolytic flux and regulation by glycerol of enzymes in Kennedy pathway for cytidine diphosphate choline production in S. cerevisiae were studied. The conventional transformation course featured four stages. Firstly, CMP and choline chloride were phosphorylated and CDP-choline was formed rapidly; secondly, the rate of CDP-choline formation declined and CMP was not detected in the mixture; thirdly, CMP was released and the CDP-choline concentration reached a peak; Fourthly, the compound concentrations did not practically changes eventually. Using the central composite design, the concentration, yield, and utilization efficiency of energy reached 24.7 mmol/L, 82.3% and 10.6%, with 30 mmol/L of ammonium ion and 1% (V/V) of glycerol, respectively. Ammonium ion not only strengthened the glycolytic pathway, but also coordinated the reaction rate between the glycolytic pathway and the Kennedy pathway. Glycerol alleviated the activity decrease of the key enzymes in the mixture.
Tang
Jiapeng
J
State Key Laboratory of Materials-Oriented Chemical Engineering, College of Life Science and Pharmaceutical Engineering, Nanjing University of Technology, No. 5 Xin Mo Fan Road, Nanjing, PR China.
Yao
Yuelan
Y
Ying
Hanjie
H
Xiong
Jian
J
Zhang
Lei
L
Li
Zhenjiang
Z
Bai
Jianxin
J
Zhang
Yeyong
Y
Ouyang
Pingkai
P
D000704:Analysis of Variance; D003470:Culture Media; D003566:Cytidine Diphosphate Choline / Q000096:biosynthesis*; D025461:Feedback, Physiological / Q000187:drug effects; D005990:Glycerol / Q000494:pharmacology*; D007477:Ions; D007700:Kinetics; D000644:Quaternary Ammonium Compounds / Q000494:pharmacology*; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism*; D013499:Surface Properties / Q000187:drug effects
D003470:Culture Media; D007477:Ions; D000644:Quaternary Ammonium Compounds; D003566:Cytidine Diphosphate Choline; D005990:Glycerol