title: Characterization of an ETS transcription factor in the sea scallop Chlamys farreri.
abstract: We have cloned and characterized a cDNA encoding a putative ETS transcription factor, designated Cf-ets. The Cf-ets encodes a 406 amino acid protein containing a conserved ETS domain and a Pointed domain. Phylogenetic analysis revealed that Cf-ets belongs to the ESE group of ETS transcription factor family. Real-time PCR analysis of Cf-ets expression in adult sea scallop tissues revealed that Cf-ets was expressed mainly in gill and hemocytes, in a constitutive manner. Cf-ets mRNA level in hemocytes increased drastically after microbial challenge indicated its indispensable role in the anti-infection process. Simultaneously, the circulating hemocyte number decreased. In mammals, most ETS transcription factors play indispensable roles in blood cell differentiation and linage commitment during hematopoisis. Cf-ets is therefore likely to be a potential biomarker for hematopoiesis studies in scallops.
authors.affiliation: Key Laboratory of Mariculture, Ministry of Education of China, Food Science and Technology College, Ocean University of China, Qingdao, China. mahongm@ouc.edu.cn
authors.forename: Hongming
authors.initials: H
authors.lastname: Ma
authors.forename: Jia
authors.initials: J
authors.lastname: Wang
authors.forename: Bing
authors.initials: B
authors.lastname: Wang
authors.forename: Yancui
authors.initials: Y
authors.lastname: Zhao
authors.forename: Changjian
authors.initials: C
authors.lastname: Yang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D005880:Gills / Q000276:immunology / Q000378:metabolism*; D006410:Hematopoiesis / Q000276:immunology / Q000502:physiology; D006434:Hemocytes / Q000276:immunology / Q000378:metabolism*; D008969:Molecular Sequence Data; D049897:Pectinidae / Q000276:immunology / Q000378:metabolism*; D010802:Phylogeny; D050783:Proto-Oncogene Proteins c-ets / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D016415:Sequence Alignment
chemical_list: D050783:Proto-Oncogene Proteins c-ets