title: Utility of 16S ribosomal DNA sequencing in the diagnosis of Staphylococcus lugdunensis native valve infective endocarditis: case report and literature review.
abstract: We report a case of possible infective endocarditis without fever presenting with an acutely ischemic limb with prior antimicrobial therapy preventing identification by culture of a microorganism. 16S ribosomal DNA sequencing led to the identification of Staphylococcus lugdunensis from an embolus removed at surgery and subsequent successful antibiotic treatment. We review the utility of 16S ribosomal DNA sequencing in diagnosing infective endocarditis and other infectious conditions.
authors.affiliation: Department of Infectious Diseases, Tan Tock Seng Hospital, 11 Jalan Tan Tock Seng, Singapore 308433. surinder_pada_kaur@ttsh.com.sg
authors.forename: Surinder
authors.initials: S
authors.lastname: Pada
authors.forename: David C
authors.initials: DC
authors.lastname: Lye
authors.forename: Yee Sin
authors.initials: YS
authors.lastname: Leo
authors.forename: Timothy
authors.initials: T
authors.lastname: Barkham
mesh_terms: D000328:Adult; D004269:DNA, Bacterial / Q000032:analysis; D004275:DNA, Ribosomal / Q000032:analysis; D004697:Endocarditis, Bacterial / Q000175:diagnosis* / Q000382:microbiology; D006349:Heart Valve Diseases / Q000175:diagnosis / Q000382:microbiology; D006351:Heart Valves / Q000382:microbiology*; D006801:Humans; D008297:Male; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D017422:Sequence Analysis, DNA / Q000379:methods*; D013203:Staphylococcal Infections / Q000175:diagnosis* / Q000382:microbiology; D013210:Staphylococcus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S