title: Quantitative determination of urea concentrations in cell culture medium.
abstract: Urea is the major nitrogenous end product of protein metabolism in mammals. Here, we describe a quantitative, sensitive method for urea determination using a modified Jung reagent. This assay is specific for urea and is unaffected by ammonia, a common interferent in tissue and cell cultures. We demonstrate that this convenient colorimetric microplate-based, room temperature assay can be applied to determine urea synthesis in cell culture.
authors.affiliation: BioAssay Systems LLC, Hayward, CA, USA.
authors.forename: Robert J X
authors.initials: RJ
authors.lastname: Zawada
authors.forename: Peggy
authors.initials: P
authors.lastname: Kwan
authors.forename: Kellen L
authors.initials: KL
authors.lastname: Olszewski
authors.forename: Manuel
authors.initials: M
authors.lastname: Llinas
authors.forename: Shu-Gui
authors.initials: SG
authors.lastname: Huang
mesh_terms: D000818:Animals; D002138:Calibration; D003470:Culture Media / Q000737:chemistry*; D010963:Plasmodium falciparum / Q000166:cytology; D012015:Reference Standards; D014508:Urea / Q000032:analysis* / Q000592:standards
chemical_list: D003470:Culture Media; D014508:Urea