title: [Level of serum and liver tissue TGF-beta1 in patients with liver fibrosis due to chronic hepatitis B].
abstract: OBJECTIVE
  To detect the level of serum and liver tissue TGF-beta1 in patients with chronic hepatitis B, to study their relation to liver fibrosis and gain the evidence for diagnosis of liver fibrosis.
  
  
  METHODS
  The liver fibrosis grades (S0-S4) of 131 cases with chronic HBV infection were diagnosed after liver biopsy. Serum TGF-beta1 was detected by enzyme-linked immunosorbent assay, and the semiquantitative analysis was applied after detecting the expression of TGF-beta1 in liver tissue with immunohistochemistry. Their relations to liver fibrosis were analyzed.
  
  
  RESULTS
  Serum and tissue level of TGF-beta1 increased significantly with the development of fibrosis, and the same result was obtained between themselves (P < 0.01). There was very significant difference for serum level of TGF-beta1 among the groups with different fibrosis grades (P < 0.01). Serum levels of TGF-beta1 were decreased significantly comparing the Group S0 or S1 to S4 (P < 0.005). There were significant difference for serum level of TGF-beta1 among S0 and the others (P < 0.005). And there was significant difference between S1 and S3 (P < 0.005). The expression level of TGF-beta1 in liver tissue has no significant difference between group S3 and S4 (P > 0.05). However, the differences were significantly among the other comparisons (P < 0.01).
  
  
  CONCLUSIONS
  There is close relation between the level of TGF-beta1 and the different liver fibrosis grades due to chronic hepatitis B. The serum level of TGF-beta1 is a potential noninvasive maker for diagnosis of liver fibrosis.
authors.affiliation: The Sixth People's Hospital of Hangzhou, Zhejiang 310014, China. guojianchun1961@126.com
authors.forename: Jian-chun
authors.initials: JC
authors.lastname: Guo
authors.forename: Jian-feng
authors.initials: JF
authors.lastname: Bao
authors.forename: Qun-wei
authors.initials: QW
authors.lastname: Chen
authors.forename: Xiao-ou
authors.initials: XO
authors.lastname: Li
authors.forename: Jun-ping
authors.initials: JP
authors.lastname: Shi
authors.forename: Guo-qiang
authors.initials: GQ
authors.lastname: Lou
authors.forename: Wei-zhen
authors.initials: WZ
authors.lastname: Shi
authors.forename: Yun-hao
authors.initials: YH
authors.lastname: Xun
mesh_terms: D000328:Adult; D005260:Female; D006509:Hepatitis B / Q000150:complications* / Q000188:drug therapy / Q000378:metabolism / Q000473:pathology; D006515:Hepatitis B virus / Q000235:genetics / Q000378:metabolism*; D019694:Hepatitis B, Chronic / Q000150:complications* / Q000378:metabolism; D006801:Humans; D008103:Liver Cirrhosis / Q000209:etiology*; D008297:Male; D053773:Transforming Growth Factor beta1 / Q000097:blood / Q000378:metabolism*
chemical_list: D053773:Transforming Growth Factor beta1