[Influences of human beta-defensin 1 on the replication and expression of HPV18 in Hela cell].
OBJECTIVE
Investigate the influences of human beta-defensinl (hbetaD1) on the replication and expression of HPV18 in Hela cell.


METHODS
Gene transfection: mediated by Fugen HD, hbetaD1/psectag plasmid was transfected to Hela cell [hbetaD1/psectag : liposome complexes (hbetaD1/psectag : lip)group], and control groups [psectag : liposome complexes(psectag : lip) group and blank group) were also established. After transfection, the expression of the target gene in Hela cell was investigated by the method of immunocytochemistry. 48 h and 72 h after the transfection, the change of the copy number of HPV18 DNA in Hela cell was investigated respectively by the quantitative fluorescent PCR method, and the change of the HPV18 E6 mRNA in Hela cell was evaluated by the semiquantitative RT-PCR.


RESULTS
48 h and 72 h after the transfection of hbetaD1/psectag plasmid to Hela cell, hbetaD1 was expressed in both of the two groups, and the latter showed a tendency of stronger expression. Compared with the control groups, the copy number of HPV18 DNA in Hela cell in the hbetaD1/psectag : lip group increased at 48 h and decreased at 72 h after the transfection, but the change was not statistically significant. 48 h after the transfection, compared with the control groups, the expression of HPV18 E6 mRNA in Hela cell in the hbetaD1/psectag : lip group changed a little; and 72 h after the transfection, the expression level of HPV18 E6 mRNA decreased significantly (P < 0.05).


CONCLUSIONS
hbetaD1 displayed an inhibitory effect to the expression of HPV18 mRNA in Hela cell in a concentration-dependent pattern, but no significant effect on the duplication of HPV18 DNA.
Zhang
Yun-yan
YY
Department of Gynecology and Obstetrics, West China Second Hospital, Sichuan University, Chengdu 610041, China.
Zheng
Ai
A
Chu
Yan-xia
YX
Zhang
Jian
J
Gong
Jun-lin
JL
Chai
Dan
D
D004279:DNA, Viral / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D005260:Female; D006367:HeLa Cells; D052161:Human papillomavirus 18 / Q000502:physiology*; D006801:Humans; D009856:Oncogene Proteins, Viral / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D002583:Uterine Cervical Neoplasms / Q000821:virology*; D014779:Virus Replication / Q000187:drug effects*; D023083:beta-Defensins / Q000494:pharmacology*
D004279:DNA, Viral; D004268:DNA-Binding Proteins; C052603:E6 protein, Human papillomavirus type 18; D009856:Oncogene Proteins, Viral; D012333:RNA, Messenger; D023083:beta-Defensins