The histological characteristics of cultured oral epithelium in different culture conditions.
This study was undertaken to establish the characterization of cultured oral mucosal epithelium and introducing them as an alternative source for reconstruction of ocular surface disease.



Human oral epithelial cells were cultured on simple media (DMEM/HF12) as control and co-cultured on mitomycin C-treated 3T3 feeder layer, on the amniotic membrane (AM) without nitrocellulose and the mitotically inactivated 3T3 fibroblast, and on the sandwich layer of AM fastened on the nitrocellulose as insert and 3T3 fibroblast. After 3 weeks, the characteristics of the cells were assessed morphologically and also ultrastructurally using scanning electron microscopy and transmission electron microscopy and immuno-cytochemically.



The epithelial cells were cultured on AM spread on nitrocellulose insert and 3T3 feeder layer showed better growth than other groups and all groups of study were shown similar characteristics. The cultured oral epithelial shared the characteristics with corneal epithelium.



Thus the oral epithelial could be an alternative source for transplantation.
Hashemi
Hassan
H
Dept. of Ophthalmology, Farabi Eye Hospital, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.|Noor Ophtalmology Research Cente ,Noor Eye Haspital, Tehran. Iran.
Salehnia
Mojdeh
M
Dept. of Anatomy, Faculty of Medical Sciences, Tarbiat Modares University, Tehran. Iran.
Kamali
Morvarid
M
Dept. of Ophthalmology, Farabi Eye Hospital, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
Beigi Boroujeni
Mandana
M
Dept. of Anatomy, Faculty of Medical Sciences, Lorestan  University of Medical Sciences, Khoramabad, Iran.
D016475:3T3 Cells; D000818:Animals; D018929:Cell Culture Techniques; D049109:Cell Proliferation; D048430:Cell Shape; D002478:Cells, Cultured; D004847:Epithelial Cells / Q000166:cytology* / Q000648:ultrastructure; D006801:Humans; D007150:Immunohistochemistry; D053551:Keratin-12 / Q000378:metabolism; D053558:Keratin-3 / Q000378:metabolism; D051379:Mice; D009061:Mouth Mucosa / Q000166:cytology* / Q000648:ultrastructure
D053551:Keratin-12; D053558:Keratin-3