[Effects of high pulsed electric field on the secondary and tertiary structure of lipoxygenase].
The effect of pulsed electric field (PEF) on the secondary and tertiary structure of lipoxygenase (LOX) in a buffer solution was analyzed using far UV-circular dichroism (CD) and fluorescence spectrophotometry, respectively. The secondary and tertiary structure of LOX changed after PEF treatment. The CD spectra of LOX also changed, with the intensity of two negative peaks and the content of alpha-helix significantly decreased (p < 0.05). The decrease in alpha-helix content in LOX showed a good linear correlation with the electric field strength. The fluorescence intensity of LOX increased, and the relative fluorescence intensity of the two characteristic peaks of LOX emission spectra at 337 nm and 583 nm also showed a good linear correlation with the electric field strength. These results showed that the activity inactivation of LOX may be due to the alteration in secondary structure, and both had a good relation.
Zhong
Kui
K
College of Food Science & Technology, Huazhong Agricultural University, Wuhan 430070, China. zhongk@mail.hzau.edu.cn
Hu
Xiao-Song
XS
Wu
Ji-Hong
JH
Chen
Fang
F
Liao
Xiao-Jun
XJ
D002942:Circular Dichroism; D004560:Electricity; D008084:Lipoxygenase / Q000737:chemistry*; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D013050:Spectrometry, Fluorescence
D008084:Lipoxygenase