Transgenic mice expressing the Nmnat1 protein manifest robust delay in axonal degeneration in vivo.
Axonal degeneration is a key component of a variety of neurological diseases. Studies using wld(s) mutant mice have demonstrated that delaying axonal degeneration slows disease course and prolongs survival in neurodegenerative disease models. The Wld(s) protein is normally localized to the nucleus, and contains the N terminus of ubiquitination factor Ube4b fused to full-length Nmnat1, an NAD biosynthetic enzyme. While Nmnat enzymatic activity is necessary for Wld(s)-mediated axonal protection, several important questions remain including whether the Ube4b component of Wld(s) also plays a role, and in which cellular compartment (nucleus vs cytosol) the axonal protective effects of Nmnat activity are mediated. While Nmnat alone is clearly sufficient to delay axonal degeneration in cultured neurons, we sought to determine whether it was also sufficient to promote axonal protection in vivo. Using cytNmnat1, an engineered mutant of Nmnat1 localized only to the cytoplasm and axon, that provides more potent axonal protection than that afforded by Wld(s) or Nmnat1, we generated transgenic mice using the prion protein promoter (PrP). The sciatic nerve of these cytNmnat1 transgenic mice was transected, and microscopic analysis of the distal nerve segment 7 d later revealed no evidence of axonal loss or myelin debris, indicating that Nmnat alone, without any other Wld(s) sequences, is all that is required to delay axonal degeneration in vivo. These results highlight the importance of understanding the mechanism of Nmnat-mediated axonal protection for the development of new treatment strategies for neurological disorders.
Sasaki
Yo
Y
Department of Pathology and Immunology, Washington University School of Medicine, St. Louis, Missouri 63110, USA.
Vohra
Bhupinder P S
BP
Baloh
Robert H
RH
Milbrandt
Jeffrey
J
D000200:Action Potentials / Q000235:genetics; D000818:Animals; D001369:Axons / Q000378:metabolism / Q000473:pathology / Q000502:physiology* / Q000648:ultrastructure; D019771:Axotomy / Q000379:methods; D001921:Brain / Q000033:anatomy & histology / Q000378:metabolism; D002478:Cells, Cultured; D003600:Cytosol / Q000378:metabolism; D005727:Ganglia, Spinal / Q000166:cytology; D018014:Gene Transfer Techniques; D049452:Green Fluorescent Proteins / Q000235:genetics; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D009410:Nerve Degeneration / Q000235:genetics* / Q000503:physiopathology / Q000517:prevention & control*; D009431:Neural Conduction / Q000502:physiology; D009612:Nicotinamide-Nucleotide Adenylyltransferase / Q000235:genetics* / Q000502:physiology; D011328:Prions / Q000235:genetics; D012584:Sciatic Nerve / Q000166:cytology / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology; D020426:Sciatic Neuropathy / Q000378:metabolism / Q000503:physiopathology; D013347:Subcellular Fractions / Q000378:metabolism; D013997:Time Factors; D046509:Tissue Culture Techniques
D011328:Prions; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins; D009612:Nicotinamide-Nucleotide Adenylyltransferase; C510972:Nmnat1 protein, mouse