[Relationship of Zn content and Na/K ratio to the resistance to pathogen invasion in antisense ACS transgenic tomato fruit and normal tomato].
In the present work, inductively coupled plasma atomic emission spectrometry (ICP-AES) was used to determine the content of Zn, Na and K and the ratio of Na/K in antisense ACS transgenic tomato fruit (in which the antisense ACC synthase gene construct was inserted) and in normal tomato fruit of the same variety (Lycopersicon esculentum cv. Lichun). The difference in mineral elements content between the two tomatoes and the involvement of Zn content and Na/K ratio in the disease resistance of postharvest tomato fruit were discussed. The results showed that the ethylene biosynthesis is limited in antisense ACS transgenic tomato fruit. Meanwhile, the resistance to Rhizopus nigricans invasion of this transgenic tomato was higher, the disease incidence and lesion area in transgenic tomato fruit were 20.0% and 33.3% lower than those in control fruit, respectively. In addition, the content of Zn in transgenic tomato fruit was 0.322 microg x g(-1) and was 1.5 times higher than that in Lichun tomato fruit. There was no significant difference in K content between transgenic tomato fruit and Lichun fruit, but the content of Na in transgenic tomato fruit was significant higher than that in Lichun fruit and the Na/K ratio in transgenic tomato fruit was 2.0 times higher than that in Lichun fruit. It is suggested that the content of Zn and the Na/K ratio may be involved in the resistance response to pathogen invasion and the development of antisense ACS transgenic tomato fruit.
Fan
Bei
B
College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China.
Shen
Lin
L
Liu
Can
C
Tian
Shi-Ping
SP
Sheng
Ji-Ping
JP
D016373:DNA, Antisense / Q000235:genetics*; D005638:Fruit / Q000235:genetics / Q000276:immunology / Q000382:microbiology / Q000502:physiology*; D007113:Immunity, Innate; D008190:Lyases / Q000235:genetics*; D018551:Solanum lycopersicum / Q000235:genetics / Q000276:immunology / Q000382:microbiology / Q000502:physiology*; D008670:Metals / Q000378:metabolism*; D010935:Plant Diseases / Q000235:genetics / Q000276:immunology / Q000382:microbiology*; D030821:Plants, Genetically Modified; D011188:Potassium / Q000378:metabolism; D012233:Rhizopus / Q000502:physiology; D012964:Sodium / Q000378:metabolism; D013054:Spectrophotometry, Atomic; D015032:Zinc / Q000378:metabolism
D016373:DNA, Antisense; D008670:Metals; D012964:Sodium; D008190:Lyases; C021773:1-aminocyclopropanecarboxylate synthase; D015032:Zinc; D011188:Potassium