Quantitative analyses of matrix metalloproteinase activity after traumatic brain injury in adult rats.
Recent laboratory evidence implicates matrix metalloproteinases (MMPs) as playing a pivotal role in ischemic and traumatic brain injuries (TBI). Here, quantitative real-time PCR analyses revealed that brains from TBI rats displayed significantly elevated MMP-9 expression at 24 h post-TBI, which remained upregulated at least until 48 h after injury. Immunohistochemical analyses similarly revealed significantly increased MMP-9 immunoreactivity at 24 and 48 h post-TBI. These results demonstrate that alterations in MMPs (i.e., MMP-9) commenced immediately after TBI, suggesting that treatment strategies designed to maintain MMP integrity should be initiated in the acute phase of injury.
Hayashi
Takuro
T
Department of Neurosurgery, University of South Florida, Tampa, FL 33612, USA.
Kaneko
Yuji
Y
Yu
SeongJin
S
Bae
EunKyung
E
Stahl
Christine E
CE
Kawase
Takeshi
T
van Loveren
Harry
H
Sanberg
Paul R
PR
Borlongan
Cesar V
CV
D000704:Analysis of Variance; D000818:Animals; D001930:Brain Injuries / Q000201:enzymology* / Q000378:metabolism; D002540:Cerebral Cortex / Q000201:enzymology* / Q000293:injuries* / Q000378:metabolism; D007150:Immunohistochemistry; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors
D012333:RNA, Messenger; D020780:Matrix Metalloproteinase 9