title: Effect of fat content on survival of Listeria monocytogenes during simulated digestion of inoculated beef frankfurters stored at 7 degrees C.
abstract: Potential effects of the fat content of frankfurters on the gastrointestinal survival of Listeria monocytogenes were investigated. At various stages of storage (7 degrees C, up to 55 days), inoculated frankfurters of low (4.5%) and high (32.5%) fat content were exposed to a dynamic gastrointestinal model (37 degrees C) and L. monocytogenes counts were determined at intervals during exposure in each gastrointestinal compartment (gastric, GC; intestinal, IC). Bacterial survival curves in each compartment were fitted with the Baranyi and Roberts mathematical model. L. monocytogenes populations on low- and high-fat frankfurters exceeded 8.0 log CFU/g at 39 and 55 days of storage, respectively. Major declines in populations occurred after 60 min on low-fat frankfurters in the GC, with reductions of 2.6 to >7.2 log CFU/g at 120 min on days 1 and 39 of storage, respectively. L. monocytogenes reductions in high-fat frankfurters ranged from 1.6 (day-1) to 5.2 (day-55) log CFU/g. Gastric inactivation rates were 0.080-0.194 and 0.030-0.097 log CFU/g/min for low- and high-fat samples, respectively. Since gastric emptying began while the gastric pH was >5, initial counts (enumerated 30 min after ingestion) reaching the IC depended on initial contamination levels on each product, which increased during storage. Subsequent reductions during the intestinal challenge were 0.1-1.4 log CFU/g. Findings indicated protective effects of fat against gastric destruction of L. monocytogenes. However, since the effects of fat were observed mainly at later stages of gastric exposure, they did not influence numbers of viable cells reaching the IC.
authors.affiliation: Center for Meat Safety & Quality, Food Safety Cluster, Department of Animal Sciences, Colorado State University, Fort Collins, CO 80523, USA.
authors.forename: Ioanna M
authors.initials: IM
authors.lastname: Barmpalia-Davis
authors.forename: Ifigenia
authors.initials: I
authors.lastname: Geornaras
authors.forename: Patricia A
authors.initials: PA
authors.lastname: Kendall
authors.forename: John N
authors.initials: JN
authors.lastname: Sofos
mesh_terms: D000818:Animals; D002417:Cattle; D003080:Cold Temperature; D015169:Colony Count, Microbial; D004041:Dietary Fats / Q000032:analysis / Q000494:pharmacology*; D004063:Digestion; D004305:Dose-Response Relationship, Drug; D005519:Food Preservation / Q000379:methods*; D005744:Gastric Acid / Q000378:metabolism; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008089:Listeria monocytogenes / Q000187:drug effects / Q000254:growth & development*; D008433:Mathematics; D008461:Meat Products / Q000032:analysis / Q000382:microbiology*; D008954:Models, Biological; D013270:Stomach / Q000737:chemistry / Q000382:microbiology*; D013997:Time Factors
chemical_list: D004041:Dietary Fats