title: A cell-based screening system for detection of inhibitors toward mycobacterial cell wall core.
abstract: Mycobacterium tuberculosis and nonpathogenic bacteria, Corynebacterium glutamicum, possess a common and unusual cell wall architecture. A cell-based screening system was designed to identify novel compounds interacting with the synthesis, assembly or regulation of the M. tuberculosis cell wall. C. glutamicum was tested in a paired medium assay in 96-well plates with natural product extracts and pure chemical compounds in the presence and absence of the osmotic stabilizer, sorbitol and some ions. Growth was visually examined over a 12-h period and detected with a microplate reader for absorbance at 544 nm. Screening hits from the osmotic stabilizer rescue were then examined by mycolic acid analysis to confirm the effect on cell wall integrity.
authors.affiliation: Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Gao
authors.forename: Yan
authors.initials: Y
authors.lastname: Guan
authors.forename: Danqing
authors.initials: D
authors.lastname: Song
authors.forename: Chunling
authors.initials: C
authors.lastname: Xiao
mesh_terms: D000904:Antibiotics, Antitubercular / Q000494:pharmacology; D002473:Cell Wall / Q000187:drug effects* / Q000648:ultrastructure*; D002855:Chromatography, Thin Layer; D048230:Corynebacterium glutamicum / Q000187:drug effects / Q000648:ultrastructure; D004232:Diuretics / Q000494:pharmacology; D004353:Drug Evaluation, Preclinical / Q000379:methods; D008826:Microbial Sensitivity Tests; D009169:Mycobacterium tuberculosis / Q000187:drug effects* / Q000648:ultrastructure*; D009171:Mycolic Acids / Q000032:analysis; D013012:Sorbitol / Q000494:pharmacology; D013056:Spectrophotometry, Ultraviolet
chemical_list: D000904:Antibiotics, Antitubercular; D004232:Diuretics; D009171:Mycolic Acids; D013012:Sorbitol