title: Efficient human growth hormone gene expression in the milk of non-transgenic goats.
abstract: Heterogenous expression of recombinant proteins in milk of livestock at a large scale is very labour-intensive to be achieved with current transgenic animals, and usually seen as time-consuming, expensive and technically most challenging. Here we describe a convenient system for transient production of recombinant human growth hormone and its extensive use in recombinant protein production for therapeutic purposes. In this study, an adenoviral vector containing the GFP gene and hGH gene was constructed for direct infusion into the epithelium of mammary glands of goats via the teat canal during the period of natural lactation. Western-blot analysis of milk samples obtained from all of the viral-treated founders indicated that the recombinant hGH (rhGH) was secreted into the milk of the goats. The concentrations of rhGH in milk ranged from 0.6 to 2.4 mg/ml and lasted for more than 10 days during lactation. These data suggest that it is possible to produce larger amounts of recombinant human growth hormone in the milk of livestock animals by using replication-defective adenoviruses.
authors.affiliation: College of Animal Science and Veterinary Medicine, Northwest A&F University, Yangling, China.
authors.forename: Z
authors.initials: Z
authors.lastname: Han
authors.forename: S
authors.initials: S
authors.lastname: Wu
authors.forename: Q
authors.initials: Q
authors.lastname: Li
authors.forename: J
authors.initials: J
authors.lastname: Li
authors.forename: D
authors.initials: D
authors.lastname: Gao
authors.forename: K
authors.initials: K
authors.lastname: Li
authors.forename: Z W
authors.initials: ZW
authors.lastname: Liu
authors.forename: H
authors.initials: H
authors.lastname: Zhao
mesh_terms: D000256:Adenoviridae / Q000235:genetics; D000818:Animals; D030801:Animals, Genetically Modified; D015153:Blotting, Western; D005260:Female; D005822:Genetic Vectors / Q000235:genetics; D006041:Goats / Q000235:genetics* / Q000378:metabolism*; D013006:Growth Hormone / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D008892:Milk / Q000378:metabolism*; D008957:Models, Genetic
chemical_list: D013006:Growth Hormone