A novel beta-glucan produced by Paenibacillus polymyxa JB115 induces nitric oxide production in RAW264.7 macrophages.
The effect of extracellular beta-(1-->3), (1-->6)-glucan, produced by Paenibacillus polymyxa JB115, on nitric oxide (NO) production in RAW264.7 macrophages was investigated. beta-glucan induced the production of NO by RAW264.7 macrophages in a concentration- and time-dependent manner. Moreover, beta-glucan stimulation increased the mRNA expression of iNOS, COX-2 and IL-6 in RAW264.7 macrophages in a concentration-dependent manner.
Chang
Zhi Qiang
ZQ
College of Veterinary Medicine, Kyungpook National University, Daegu, Korea.
Lee
Joong Su
JS
Hwang
Mi Hyun
MH
Hong
Joo Heon
JH
Jung
Hee Kyoung
HK
Lee
Sam Pin
SP
Park
Seung Chun
SC
D000818:Animals; D001407:Bacillus / Q000378:metabolism*; D002460:Cell Line; D051546:Cyclooxygenase 2 / Q000096:biosynthesis / Q000235:genetics; D015850:Interleukin-6 / Q000096:biosynthesis / Q000235:genetics; D008070:Lipopolysaccharides / Q000494:pharmacology; D008264:Macrophages / Q000187:drug effects* / Q000201:enzymology / Q000276:immunology; D051379:Mice; D009569:Nitric Oxide / Q000096:biosynthesis* / Q000276:immunology; D052247:Nitric Oxide Synthase Type II / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D047071:beta-Glucans / Q000378:metabolism / Q000494:pharmacology*
D015850:Interleukin-6; D008070:Lipopolysaccharides; D012333:RNA, Messenger; D047071:beta-Glucans; D009569:Nitric Oxide; D052247:Nitric Oxide Synthase Type II; C077623:Ptgs2 protein, mouse; D051546:Cyclooxygenase 2