Characterization of crustin antimicrobial proteins from Japanese spiny lobster Panulirus japonicus.
Crustin antimicrobial proteins (PJC1-4) were identified from a phyllosoma library of Japanese spiny lobster, Panulirus japonicus. The deduced amino acid sequences of PJC1-4 contained open reading frames of 130, 139, 124 and 150 amino acid residues, respectively. These proteins contained a glycine-rich region at the N-terminus and 12 conserved cysteine residues containing a single whey acidic protein (WAP) domain at the C-terminus. A phylogenetic tree and sequences alignment analyses revealed that PJC1-4 are more closely related to shrimp crustins than to other lobster crustins. Transcripts of PJC1, 3 and 4 were detected in heart, nerves, intestine, hemocytes, gills and hepatopancreas, while transcripts of PJC2 were detected only in nerves.
Pisuttharachai
Duangjai
D
Laboratory of Genome Science, Tokyo University of Marine Science and Technology, Konan 4-5-7, Minato-ku, Tokyo, 108-8477, Japan.
Fagutao
Fernand F
FF
Yasuike
Motoshige
M
Aono
Hideaki
H
Yano
Yutaka
Y
Murakami
Keisuke
K
Kondo
Hidehiro
H
Aoki
Takashi
T
Hirono
Ikuo
I
D000595:Amino Acid Sequence; D000818:Animals; D023181:Antimicrobial Cationic Peptides / Q000145:classification / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D020869:Gene Expression Profiling; D008969:Molecular Sequence Data; D033521:Palinuridae / Q000235:genetics*; D010802:Phylogeny; D020033:Protein Isoforms / Q000145:classification / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D014158:Transcription, Genetic
D023181:Antimicrobial Cationic Peptides; D018076:DNA, Complementary; D020033:Protein Isoforms; D012333:RNA, Messenger