title: Influence of ligand density on antibody binding capacity of cation-exchange adsorbents.
abstract: Adsorption properties of a set of polymethacrylate-based cation exchangers designed for purification of monoclonal antibodies were investigated. The materials differed significantly in the density of sulphoisobutyl ligand groups. The ligand density had a pronounced effect on the static adsorption capacity of a polyclonal human immunoglobulin G. An optimal ligand density was observed at any pH and NaCl concentration tested when sharp optima were observed at low pH and ionic strength values. This was caused by effective clogging of pore mouth at high ligand densities. An anomalous effect of ionic strength was observed for the adsorbents with the high ligand density when the adsorption capacity increased with the addition of NaCl at low pH.
authors.affiliation: Department of Chemical and Biochemical Engineering, Faculty of Chemical and Food Technology, Slovak University of Technology, Bratislava, Slovakia.
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Wrzosek
authors.forename: Michal
authors.initials: M
authors.lastname: Gramblicka
authors.forename: Milan
authors.initials: M
authors.lastname: Polakovic
mesh_terms: D000327:Adsorption; D000911:Antibodies, Monoclonal / Q000032:analysis / Q000378:metabolism; D002411:Cation Exchange Resins / Q000378:metabolism*; D002852:Chromatography, Ion Exchange; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007074:Immunoglobulin G / Q000032:analysis / Q000378:metabolism; D008024:Ligands; D009994:Osmolar Concentration; D011485:Protein Binding; D012965:Sodium Chloride / Q000737:chemistry
chemical_list: D000911:Antibodies, Monoclonal; D002411:Cation Exchange Resins; D007074:Immunoglobulin G; D008024:Ligands; D012965:Sodium Chloride