Down-regulation of APLP1 mRNA expression in hippocampus of pilocarpine-induced epileptic rats.
OBJECTIVE
To investigate the expression of amyloid beta precursor-like protein 1(APLP1) gene on the transcription level in hippocampus of pilocarpine-induced epileptic rats.


METHODS
Epileptic rats were developed by LiC1 (3 mmol/kg, i.p.) approximately 20 h prior to pilocarpine (30 mg/kg, i.p.) administration. The 3' end partial sequence of rat APLP1 gene was cloned, and the expression levels of APLP1 mRNA in hippocampus of epileptic rats at 6 h, 30 h, 7 d and 15 d were determined by semi-quantitative RT-PCR.


RESULTS
The 3'end partial sequence of rat APLP1 gene shared a 97% homology with that of mice, and 90% with that of human. The APLP1 amino acid sequence of rat was identical with that of mouse, but was different from that of human in 3 residues. Moreover, pilocarpine induced a significant down-regulation of APLP1 mRNA expression at 6 h after epilepsy initiation (P< 0.05), and at 30 h, this down-regulation became more dramatic (P< 0.01), which lasted till day 15 (P< 0.01).


CONCLUSIONS
The 3' end of APLP1 gene is highly conserved, and APLP1 mRNA expression is kept at low level in hippocampus of pilocarpine-induced epileptic rats.
Wang
Cheng
C
Key Laboratory for Neuroinformation of Ministry of Education, School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu, 610054, China.
You
Zi-Li
ZL
Zhang
Ding-Ding
DD
D000595:Amino Acid Sequence; D016564:Amyloid beta-Protein Precursor / Q000235:genetics* / Q000378:metabolism; D000818:Animals; D001483:Base Sequence; D004195:Disease Models, Animal; D015536:Down-Regulation / Q000502:physiology*; D004827:Epilepsy / Q000139:chemically induced / Q000473:pathology*; D006624:Hippocampus / Q000378:metabolism*; D006801:Humans; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D010862:Pilocarpine; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D016415:Sequence Alignment; D013997:Time Factors
D016564:Amyloid beta-Protein Precursor; D012333:RNA, Messenger; D010862:Pilocarpine