Determination of tocopheryl acetate and ascorbyl tetraisopalmitate in cosmetic formulations by HPLC.
A rapid HPLC method was developed for the assay of tocopheryl acetate and ascorbyl tetraisopalmitate in cosmetic formulations. The validated method was applied for quantitative determination of these vitamins in simulated emulsion formulation. Samples were analysed directly on a RP-18 reverse phase column with UV detection at 222 nm. A mixture of methanol and isopropanol (25 : 75 v/v) was used as mobile phase. The retention time of tocopheryl acetate and ascorbyl tetraisopalmitate were 3.0 min and 5.9 min, respectively. Recovery was between 95% and 104%. In addition, the excipients did not interfere in the analysis. The method is simple, reproducible, selective and is suitable for routine analyses of commercial products.
Almeida
M M
MM
Department of Pharmacy, Faculty of Pharmaceutical Sciences, University of São Paulo, CEP 05508-900, São Paulo, Brazil. marimandelli@usp.br
Alves
J M P
JM
Patto
D C S
DC
Lima
C R R C
CR
Quenca-Guillen
J S
JS
Santoro
M I R M
MI
Kedor-Hackmann
E R M
ER
D000975:Antioxidants / Q000032:analysis*; D001205:Ascorbic Acid / Q000031:analogs & derivatives* / Q000032:analysis; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003358:Cosmetics / Q000737:chemistry*; D024505:Tocopherols / Q000032:analysis*
D000975:Antioxidants; D003358:Cosmetics; D001205:Ascorbic Acid; C031226:6-O-palmitoylascorbic acid; D024505:Tocopherols