[Influence of cultivation temperature of Yersinia pseudotuberculosis on the immunobiological properties of lipopolysaccharide].
OBJECTIVE
To study relationship between Yersinia pseudotuberculosis cultivation temperature and immunobiological properties of lypopolysaccharide (LPS).


METHODS
LPS producing strain--Yersinia pseudotuberculosis 164/84 OX; experimental animal--guinea pigs, rabbits; methods--immunochemical (indirect hemagglutionation assay, RDP), physico-chemical (electrophoresis, gas-liquid chromatography), standard biochemical and statistical methods.


RESULTS
It was established that increase of Yersinia pseudotuberculosis cultivation temperature from 10 degrees C to 37 degrees C was associated with attenuation of LPS toxicity in guinea pigs, decrease of its immunochemical activity and contents and degree of acylation of 3OH-14:0 hydoxyacid as well as amount of electrophoretically detected O-side chains. It was assumed that the polysaccharide component plays its role in LPS toxicity.


CONCLUSIONS
Relation between Yersinia pseudotuberculosis cultivation temperature and structural and functional properties of LPS preparations obtained from the culture was determined.
Byvalov
A A
AA
Eremenko
Iu D
IuD
Drobkov
V I
VI
Smirnova
I V
IV
Gavrilova
L B
LB
Pechenkin
D V
DV
Kozhemiako
A V
AV
Bobrova
L G
LG
D000215:Acylation; D000429:Alcohol Oxidoreductases / Q000378:metabolism; D000818:Animals; D011232:Chemical Precipitation; D006168:Guinea Pigs; D007106:Immune Sera / Q000276:immunology; D007928:Lethal Dose 50; D008050:Lipid A / Q000737:chemistry / Q000276:immunology* / Q000633:toxicity; D011817:Rabbits; D013696:Temperature; D015011:Yersinia pseudotuberculosis / Q000254:growth & development* / Q000276:immunology*
D007106:Immune Sera; D008050:Lipid A; D000429:Alcohol Oxidoreductases