title: SECIS-binding protein 2, a key player in selenoprotein synthesis, is an intrinsically disordered protein.
abstract: Selenocysteine (Sec) is co-translationally incorporated into selenoproteins at a reprogrammed UGA codon. In mammals, this requires a dedicated machinery comprising a stem-loop structure in the 3' UTR RNA (the SECIS element) and the specific SECIS Binding Protein 2. In this report, disorder-prediction methods and several biophysical techniques showed that ca. 70% of the SBP2 sequence is disordered, whereas the RNA binding domain appears to be folded and functional. These results are consistent with a recent report on the role of the Hsp90 chaperone for the folding of SBP2 and other functionally unrelated proteins bearing an RNA binding domain homologous to SBP2.
authors.affiliation: Architecture et Réactivité de l'ARN, Université de Strasbourg, CNRS, IBMC, France.
authors.forename: Vincent
authors.initials: V
authors.lastname: Oliéric
authors.forename: Philippe
authors.initials: P
authors.lastname: Wolff
authors.forename: Akiko
authors.initials: A
authors.lastname: Takeuchi
authors.forename: Guillaume
authors.initials: G
authors.lastname: Bec
authors.forename: Catherine
authors.initials: C
authors.lastname: Birck
authors.forename: Marc
authors.initials: M
authors.lastname: Vitorino
authors.forename: Bruno
authors.initials: B
authors.lastname: Kieffer
authors.forename: Artemy
authors.initials: A
authors.lastname: Beniaminov
authors.forename: Giorgio
authors.initials: G
authors.lastname: Cavigiolio
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Theil
authors.forename: Christine
authors.initials: C
authors.lastname: Allmang
authors.forename: Alain
authors.initials: A
authors.lastname: Krol
authors.forename: Philippe
authors.initials: P
authors.lastname: Dumas
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D006801:Humans; D008969:Molecular Sequence Data; D011489:Protein Denaturation; D016601:RNA-Binding Proteins / Q000737:chemistry* / Q000378:metabolism*; D051381:Rats; D051140:Selenoproteins / Q000096:biosynthesis*; D017422:Sequence Analysis, DNA
chemical_list: D016601:RNA-Binding Proteins; C498488:Secisbp2 protein, rat; D051140:Selenoproteins