[Analysis of monosaccharides in Radix Rehmanniae by GC].
OBJECTIVE
To isolate and purify the polysaccharides from Radix Rehmanniae and analysis the monosaccharides composition.


METHODS
The polysaccharides were extracted with hot water and precipitated by alcohol. Proteins in the precipitates were removed by TCA method. The products were further purified with column chromatography on Superdex 200 and Sephadex G100. The SRP I and SRP II were identified as homogeneous polysaccharide by HPLC, respectively, and then analyzed by GC after being hydrolysised.


RESULTS
Two homogeneous polysaccharides (SRP I and SRP II) were obtained from Radix Rehmanniae.


CONCLUSIONS
SRP I contained rhamnose, arabinose, glucose and galactose in the percentage of 6.11%, 66.46%, 3.93% and 21.50%. SRP I was composed of rhamnose, fucose, mannose, galactose and fructose in the percentage of 21.82%, 24.47%, 10.48%, 29.94% and 13.29%.
Zhang
Yanping
Y
Zhejiang University of Science and Technology, Hangzhou 310012, China. zhangya5437@sina.com
Yu
Yuanzhi
Y
Zhang
Hong
H
D001089:Arabinose / Q000737:chemistry / Q000302:isolation & purification; D002849:Chromatography, Gas / Q000379:methods*; D019411:Clinical Laboratory Techniques; D004365:Drugs, Chinese Herbal / Q000032:analysis; D005632:Fructose / Q000737:chemistry / Q000302:isolation & purification; D005643:Fucose / Q000737:chemistry / Q000302:isolation & purification; D005690:Galactose / Q000737:chemistry / Q000302:isolation & purification; D005947:Glucose / Q000737:chemistry / Q000302:isolation & purification; D008358:Mannose / Q000737:chemistry / Q000302:isolation & purification; D009005:Monosaccharides / Q000737:chemistry / Q000302:isolation & purification*; D010936:Plant Extracts / Q000737:chemistry; D011134:Polysaccharides / Q000737:chemistry / Q000302:isolation & purification; D012210:Rhamnose / Q000737:chemistry / Q000302:isolation & purification; D028083:Scrophulariaceae / Q000737:chemistry*
D004365:Drugs, Chinese Herbal; D009005:Monosaccharides; D010936:Plant Extracts; D011134:Polysaccharides; D005643:Fucose; D005632:Fructose; D001089:Arabinose; D005947:Glucose; D008358:Mannose; D012210:Rhamnose; D005690:Galactose