title: IL-17 potentiates neuronal injury induced by oxygen-glucose deprivation and affects neuronal IL-17 receptor expression.
abstract: Interleukin-17 (IL-17) is active in a variety of brain injuries, including ischemia. The objective of this study was to test the hypothesis that IL-17 potentiates neuronal injury after stroke. Increased expression of IL-17 and IL-17 receptor (IL-17R) in serum and cortex was evaluated by ELISA, RT-PCR and immunohistochemistry. In the in vitro model of oxygen-glucose deprivation (OGD), IL-17 showed a dose-dependent effect in promoting neuronal injury through IL-17-IL-17R combination which can be blocked by IL-17R/Fc chimera. Our results demonstrated the up-regulation of IL-17 and IL-17R following permanent middle cerebral artery occlusion and suggested that they contributed to stroke outcome.
authors.affiliation: Department of Neurobiology, Harbin Medical University, Heilongjiang Provincial Key Laboratory of Neurobiology, 157 Bao Jian Road, Harbin, China, 150081.
authors.forename: Dan-dan
authors.initials: DD
authors.lastname: Wang
authors.forename: Yan-feng
authors.initials: YF
authors.lastname: Zhao
authors.forename: Guang-you
authors.initials: GY
authors.lastname: Wang
authors.forename: Bo
authors.initials: B
authors.lastname: Sun
authors.forename: Qing-fei
authors.initials: QF
authors.lastname: Kong
authors.forename: Kai
authors.initials: K
authors.lastname: Zhao
authors.forename: Yao
authors.initials: Y
authors.lastname: Zhang
authors.forename: Jing-hua
authors.initials: JH
authors.lastname: Wang
authors.forename: Yu-mei
authors.initials: YM
authors.lastname: Liu
authors.forename: Li-li
authors.initials: LL
authors.lastname: Mu
authors.forename: De-sheng
authors.initials: DS
authors.lastname: Wang
authors.forename: Hu-lun
authors.initials: HL
authors.lastname: Li
mesh_terms: D000818:Animals; D017209:Apoptosis; D002545:Brain Ischemia / Q000473:pathology*; D015687:Cell Hypoxia; D002478:Cells, Cultured; D005947:Glucose / Q000378:metabolism*; D006624:Hippocampus / Q000473:pathology; D006801:Humans; D020381:Interleukin-17 / Q000032:analysis / Q000235:genetics / Q000502:physiology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009474:Neurons / Q000473:pathology*; D053722:Receptors, Interleukin-17 / Q000032:analysis / Q000235:genetics / Q000502:physiology*
chemical_list: D020381:Interleukin-17; D053722:Receptors, Interleukin-17; D005947:Glucose