Effects of zinc toxicity on sugar beet (Beta vulgaris L.) plants grown in hydroponics.
The effects of high Zn concentration were investigated in sugar beet (Beta vulgaris L.) plants grown in a controlled environment in hydroponics. High concentrations of Zn sulphate in the nutrient solution (50, 100 and 300 microm) decreased root and shoot fresh and dry mass, and increased root/shoot ratios, when compared to control conditions (1.2 microm Zn). Plants grown with excess Zn had inward-rolled leaf edges and a damaged and brownish root system, with short lateral roots. High Zn decreased N, Mg, K and Mn concentrations in all plant parts, whereas P and Ca concentrations increased, but only in shoots. Leaves of plants treated with 50 and 100 microm Zn developed symptoms of Fe deficiency, including decreases in Fe, chlorophyll and carotenoid concentrations, increases in carotenoid/chlorophyll and chlorophyll a/b ratios and de-epoxidation of violaxanthin cycle pigments. Plants grown with 300 microm Zn had decreased photosystem II efficiency and further growth decreases but did not have leaf Fe deficiency symptoms. Leaf Zn concentrations of plants grown with excess Zn were high but fairly constant (230-260 microg.g(-1) dry weight), whereas total Zn uptake per plant decreased markedly with high Zn supply. These data indicate that sugar beet could be a good model to investigate Zn homeostasis mechanisms in plants, but is not an efficient species for Zn phytoremediation.
Sagardoy
R
R
Departamento de Nutrición Vegetal, Estación Experimental de Aula Dei, Consejo Superior de Investigaciones Científicas, Zaragoza, Spain.
Morales
F
F
López-Millán
A-F
AF
Abadía
A
A
Abadía
J
J
D027461:Beta vulgaris / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D001692:Biological Transport / Q000187:drug effects; D002245:Carbon Dioxide / Q000378:metabolism; D002734:Chlorophyll / Q000378:metabolism; D000077194:Chlorophyll A; D038181:FMN Reductase / Q000378:metabolism; D018527:Hydroponics; D008903:Minerals / Q000378:metabolism; D009584:Nitrogen / Q000378:metabolism; D010100:Oxygen / Q000378:metabolism; D045332:Photosystem II Protein Complex / Q000187:drug effects / Q000502:physiology; D018514:Plant Structures / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D014131:Trace Elements / Q000378:metabolism; D024341:Xanthophylls / Q000378:metabolism; D015032:Zinc / Q000378:metabolism / Q000633:toxicity*; D019287:Zinc Sulfate / Q000378:metabolism / Q000633:toxicity*
D008903:Minerals; D045332:Photosystem II Protein Complex; D014131:Trace Elements; D024341:Xanthophylls; D002734:Chlorophyll; D002245:Carbon Dioxide; C005613:violaxanthin; C037184:chlorophyll b; D019287:Zinc Sulfate; D038181:FMN Reductase; C023903:ferric citrate iron reductase; D015032:Zinc; D009584:Nitrogen; D010100:Oxygen; D000077194:Chlorophyll A