title: PI(3,4,5)P3 Interactome.
abstract: Immobilizing chemically synthesized analogues of PI(3,4,5)P3 onto Affi-10 beads and incorporating them into liposomes allowed their use as affinity absorbents in the comprehensive analysis of the phosphoinositide interactome using cytosolic cell extracts of the LIM1215 colon cancer cell line. This led to the identification of 282 proteins that either interact with PI(3,4,5)P3 or are indirectly captured as part of a complex containing a PI(3,4,5)P3 binding partner. Identification of the proteins was achieved using affinity/LC-MS/MS experiments.
authors.affiliation: Ludwig Institute for Cancer Research, Melbourne Tumour Biology Branch, Royal Melbourne Hospital, and School of Chemistry, Bio21 Institute, University of Melbourne, Parkville, Victoria 3010, Australia. Bruno.catimel@ludwig.edu.au
authors.forename: Bruno
authors.initials: B
authors.lastname: Catimel
authors.forename: Meng-Xin
authors.initials: MX
authors.lastname: Yin
authors.forename: Christine
authors.initials: C
authors.lastname: Schieber
authors.forename: Melanie
authors.initials: M
authors.lastname: Condron
authors.forename: Heather
authors.initials: H
authors.lastname: Patsiouras
authors.forename: Jenny
authors.initials: J
authors.lastname: Catimel
authors.forename: Diane E J E
authors.initials: DE
authors.lastname: Robinson
authors.forename: Leon S-M
authors.initials: LS
authors.lastname: Wong
authors.forename: Edouard C
authors.initials: EC
authors.lastname: Nice
authors.forename: Andrew B
authors.initials: AB
authors.lastname: Holmes
authors.forename: Antony W
authors.initials: AW
authors.lastname: Burgess
mesh_terms: D002277:Carcinoma / Q000378:metabolism*; D045744:Cell Line, Tumor; D003110:Colonic Neoplasms / Q000378:metabolism*; D019295:Computational Biology / Q000379:methods*; D003600:Cytosol / Q000378:metabolism; D005982:Glutathione Transferase / Q000378:metabolism; D006801:Humans; D008081:Liposomes / Q000737:chemistry; D013058:Mass Spectrometry / Q000379:methods; D008956:Models, Chemical; D010710:Phosphates / Q000737:chemistry; D018129:Phosphatidylinositol Phosphates / Q000737:chemistry* / Q000378:metabolism; D025941:Protein Interaction Mapping; D020543:Proteome; D040901:Proteomics / Q000379:methods*
chemical_list: D008081:Liposomes; D010710:Phosphates; D018129:Phosphatidylinositol Phosphates; D020543:Proteome; C060974:phosphatidylinositol 3,4,5-triphosphate; D005982:Glutathione Transferase