title: Genome sequence of the recombinant protein production host Pichia pastoris.
abstract: The methylotrophic yeast Pichia pastoris is widely used for the production of proteins and as a model organism for studying peroxisomal biogenesis and methanol assimilation. P. pastoris strains capable of human-type N-glycosylation are now available, which increases the utility of this organism for biopharmaceutical production. Despite its biotechnological importance, relatively few genetic tools or engineered strains have been generated for P. pastoris. To facilitate progress in these areas, we present the 9.43 Mbp genomic sequence of the GS115 strain of P. pastoris. We also provide manually curated annotation for its 5,313 protein-coding genes.
authors.affiliation: Unit for Molecular Glycobiology, Department for Molecular Biomedical Research, VIB, Ghent-Zwijnaarde, Belgium.
authors.forename: Kristof
authors.initials: K
authors.lastname: De Schutter
authors.forename: Yao-Cheng
authors.initials: YC
authors.lastname: Lin
authors.forename: Petra
authors.initials: P
authors.lastname: Tiels
authors.forename: Annelies
authors.initials: A
authors.lastname: Van Hecke
authors.forename: Sascha
authors.initials: S
authors.lastname: Glinka
authors.forename: Jacqueline
authors.initials: J
authors.lastname: Weber-Lehmann
authors.forename: Pierre
authors.initials: P
authors.lastname: Rouzé
authors.forename: Yves
authors.initials: Y
authors.lastname: Van de Peer
authors.forename: Nico
authors.initials: N
authors.lastname: Callewaert
mesh_terms: D001483:Base Sequence; D003062:Codon / Q000235:genetics; D005800:Genes, Fungal; D016681:Genome, Fungal; D053858:Metabolic Networks and Pathways / Q000235:genetics; D008969:Molecular Sequence Data; D010802:Phylogeny; D010843:Pichia / Q000235:genetics*; D012343:RNA, Transfer / Q000235:genetics; D011994:Recombinant Proteins; D017422:Sequence Analysis, DNA
chemical_list: D003062:Codon; D011994:Recombinant Proteins; D012343:RNA, Transfer