title: CD2 distinguishes two subsets of human plasmacytoid dendritic cells with distinct phenotype and functions.
abstract: Plasmacytoid dendritic cells (pDCs) are key regulators of antiviral immunity. They rapidly secrete IFN-alpha and cross-present viral Ags, thereby launching adaptive immunity. In this study, we show that activated human pDCs inhibit replication of cancer cells and kill them in a contact-dependent fashion. Expression of CD2 distinguishes two pDC subsets with distinct phenotype and function. Both subsets secrete IFN-alpha and express granzyme B and TRAIL. CD2(high) pDCs uniquely express lysozyme and can be found in tonsils and in tumors. Both subsets launch recall T cell responses. However, CD2(high) pDCs secrete higher levels of IL12p40, express higher levels of costimulatory molecule CD80, and are more efficient in triggering proliferation of naive allogeneic T cells. Thus, human blood pDCs are composed of subsets with specific phenotype and functions.
authors.affiliation: Baylor-National Institute of Allergy and Infectious Diseases, Cooperative Center for Translational Research on Human Immunology and Biodefense, Dallas, TX 75204, USA.
authors.forename: Toshimichi
authors.initials: T
authors.lastname: Matsui
authors.forename: John E
authors.initials: JE
authors.lastname: Connolly
authors.forename: Mark
authors.initials: M
authors.lastname: Michnevitz
authors.forename: Damien
authors.initials: D
authors.lastname: Chaussabel
authors.forename: Chun-I
authors.initials: CI
authors.lastname: Yu
authors.forename: Casey
authors.initials: C
authors.lastname: Glaser
authors.forename: Sasha
authors.initials: S
authors.lastname: Tindle
authors.forename: Marc
authors.initials: M
authors.lastname: Pypaert
authors.forename: Heidi
authors.initials: H
authors.lastname: Freitas
authors.forename: Bernard
authors.initials: B
authors.lastname: Piqueras
authors.forename: Jacques
authors.initials: J
authors.lastname: Banchereau
authors.forename: A Karolina
authors.initials: AK
authors.lastname: Palucka
mesh_terms: D018122:B7-1 Antigen / Q000032:analysis; D018801:CD2 Antigens; D049109:Cell Proliferation; D003602:Cytotoxicity, Immunologic; D003713:Dendritic Cells / Q000166:cytology* / Q000276:immunology; D006801:Humans; D053762:Interleukin-12 Subunit p40 / Q000032:analysis; D009369:Neoplasms / Q000276:immunology; D010641:Phenotype; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology
chemical_list: D018122:B7-1 Antigen; D018801:CD2 Antigens; C500879:IL12B protein, human; D053762:Interleukin-12 Subunit p40