title: Chromatin-level regulation of  biosynthetic gene clusters.
abstract: Loss-of-function Aspergillus nidulans CclA, a Bre2 ortholog involved in histone H3 lysine 4 methylation, activated the expression of cryptic secondary metabolite clusters in A. nidulans. One new cluster generated monodictyphenone, emodin and emodin derivatives, whereas a second encoded two anti-osteoporosis polyketides, F9775A and F9775B. Modification of the chromatin landscape in fungal secondary metabolite clusters allows for a simple technological means to express silent fungal secondary metabolite gene clusters.
authors.affiliation: Department of Medical Microbiology and Immunology (formerly Department of Plant Pathology), University of Wisconsin-Madison, Madison, Wisconsin, USA.
authors.forename: Jin Woo
authors.initials: JW
authors.lastname: Bok
authors.forename: Yi-Ming
authors.initials: YM
authors.lastname: Chiang
authors.forename: Edyta
authors.initials: E
authors.lastname: Szewczyk
authors.forename: Yazmid
authors.initials: Y
authors.lastname: Reyes-Dominguez
authors.forename: Ashley D
authors.initials: AD
authors.lastname: Davidson
authors.forename: James F
authors.initials: JF
authors.lastname: Sanchez
authors.forename: Hsien-Chun
authors.initials: HC
authors.lastname: Lo
authors.forename: Kenji
authors.initials: K
authors.lastname: Watanabe
authors.forename: Joseph
authors.initials: J
authors.lastname: Strauss
authors.forename: Berl R
authors.initials: BR
authors.lastname: Oakley
authors.forename: Clay C C
authors.initials: CC
authors.lastname: Wang
authors.forename: Nancy P
authors.initials: NP
authors.lastname: Keller
mesh_terms: D000900:Anti-Bacterial Agents / Q000096:biosynthesis / Q000737:chemistry; D001233:Aspergillus nidulans / Q000201:enzymology / Q000235:genetics* / Q000378:metabolism; D002843:Chromatin / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D055808:Drug Discovery; D004642:Emodin / Q000737:chemistry; D017353:Gene Deletion; D015966:Gene Expression Regulation, Fungal; D006657:Histones / Q000378:metabolism; D047468:Immunoprecipitation; D018942:Macrolides / Q000737:chemistry; D013058:Mass Spectrometry; D008745:Methylation; D015394:Molecular Structure; D005810:Multigene Family; D048630:Polyketide Synthases / Q000235:genetics; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic
chemical_list: D000900:Anti-Bacterial Agents; D002843:Chromatin; D006657:Histones; D018942:Macrolides; D048630:Polyketide Synthases; D004642:Emodin