Expression of nucleus-encoded genes for chloroplast proteins in the flagellate Euglena gracilis.
Reverse transcription PCRs (RT-PCRs), real-time RT-PCRs and microarrays containing 50-mer oligonucleotides representing nucleus-encoded genes for chloroplast proteins from Euglena gracilis were used to compare light- and dark-grown wild-type mRNA levels to those of light- and dark-grown E. gracilis stable white mutant strains W(gm)ZOflL, W₃BUL and W₁₀BSmL. The analyses revealed no light-dependent regulation of mRNA levels. Moreover, the mRNA levels of most genes were unchanged in all white mutants in comparison with wild-type. These results suggest that mRNA levels of nucleus-encoded genes for chloroplast proteins in E. gracilis do not depend on either light or plastid function.
Vesteg
Matej
M
Institute of Cell Biology, Faculty of Natural Sciences, Comenius University, 84215 Bratislava, Slovakia.
Vacula
Rostislav
R
Burey
Suzanne
S
Löffelhardt
Wolfgang
W
Drahovská
Hana
H
Martin
William
W
Krajcovic
Juraj
J
D001483:Base Sequence; D002467:Cell Nucleus / Q000235:genetics*; D002736:Chloroplasts / Q000737:chemistry / Q000378:metabolism*; D018742:DNA, Chloroplast / Q000235:genetics; D005056:Euglena gracilis / Q000166:cytology* / Q000235:genetics* / Q000378:metabolism; D008027:Light; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D018747:RNA, Chloroplast / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis
D018742:DNA, Chloroplast; D010940:Plant Proteins; D018747:RNA, Chloroplast; D012333:RNA, Messenger