Evaluation of radiation-induced hair follicle apoptosis in mice and the preventive effects of fibroblast growth factor-1.
Radiation-induced hair loss is a clinically important, but under-researched topic. The aim of the study was to develop an in vivo assay system for radiation-induced apoptosis in hair follicles to promote hair research and exploit new radioprotectors. BALB/c mice received total body irradiation (TBI) with gamma-rays at doses in the range from 8 to 16 Gy at 6 days after depilation. Pathological changes were detected progressively in the hair follicles over the time course after TBI and the dystrophy was evaluated on the basis of stage-specific parameters reported previously, which were found to be well-suited for classification of the radiation-induced hair follicle dystrophy. As a result, regression from anagen to catagen was determined in these follicles after irradiation. In addition, radiation-induced apoptosis was a good early dystrophic parameter. In this system, it was found that fibroblast growth factor-1 effectively prevented hair follicle apoptosis in mice.
Nakayama
Fumiaki
F
Hagiwara
Akiko
A
Kimura
Miho
M
Akashi
Makoto
M
Imamura
Toru
T
D000818:Animals; D017209:Apoptosis / Q000187:drug effects* / Q000528:radiation effects*; D053148:Caspase 3 / Q000378:metabolism; D016220:Fibroblast Growth Factor 1 / Q000494:pharmacology* / Q000627:therapeutic use*; D005720:Gamma Rays; D018859:Hair Follicle / Q000378:metabolism / Q000473:pathology* / Q000528:radiation effects*; D006204:Hair Removal; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D011833:Radiation Injuries, Experimental / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*; D014916:Whole-Body Irradiation
D016220:Fibroblast Growth Factor 1; D053148:Caspase 3