Chromatin-level regulation of  biosynthetic gene clusters.
Loss-of-function Aspergillus nidulans CclA, a Bre2 ortholog involved in histone H3 lysine 4 methylation, activated the expression of cryptic secondary metabolite clusters in A. nidulans. One new cluster generated monodictyphenone, emodin and emodin derivatives, whereas a second encoded two anti-osteoporosis polyketides, F9775A and F9775B. Modification of the chromatin landscape in fungal secondary metabolite clusters allows for a simple technological means to express silent fungal secondary metabolite gene clusters.
Bok
Jin Woo
JW
Department of Medical Microbiology and Immunology (formerly Department of Plant Pathology), University of Wisconsin-Madison, Madison, Wisconsin, USA.
Chiang
Yi-Ming
YM
Szewczyk
Edyta
E
Reyes-Dominguez
Yazmid
Y
Davidson
Ashley D
AD
Sanchez
James F
JF
Lo
Hsien-Chun
HC
Watanabe
Kenji
K
Strauss
Joseph
J
Oakley
Berl R
BR
Wang
Clay C C
CC
Keller
Nancy P
NP
D000900:Anti-Bacterial Agents / Q000096:biosynthesis / Q000737:chemistry; D001233:Aspergillus nidulans / Q000201:enzymology / Q000235:genetics* / Q000378:metabolism; D002843:Chromatin / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D055808:Drug Discovery; D004642:Emodin / Q000737:chemistry; D017353:Gene Deletion; D015966:Gene Expression Regulation, Fungal; D006657:Histones / Q000378:metabolism; D047468:Immunoprecipitation; D018942:Macrolides / Q000737:chemistry; D013058:Mass Spectrometry; D008745:Methylation; D015394:Molecular Structure; D005810:Multigene Family; D048630:Polyketide Synthases / Q000235:genetics; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic
D000900:Anti-Bacterial Agents; D002843:Chromatin; D006657:Histones; D018942:Macrolides; D048630:Polyketide Synthases; D004642:Emodin