title: Linear disulfide-containing low polymer as efficient DNA cleavage reagent.
abstract: A novel linear poly[1,7-bis(mercaptoacetyl)-1,4,7,10-tetraazacyclododecane] (PBMAC) containing macrocyclic polyamine was synthesized through oxidation of 1,7-bis(mercaptoacetyl)-1,4,7,10-tetraazacyclododecane (BMAC) and characterized. Gel electrophoresis experiments showed that PBMAC can promote the DNA cleavage more efficiently than its monomer under physiological conditions without any thiol additives. Fluorescence quenching and DNA melting experiments demonstrated that PBMAC has stronger binding ability with DNA than that of monomer.
authors.affiliation: Key Laboratory of Green Chemistry and Technology, Ministry of Education, College of Chemistry, Sichuan University, Chengdu 610064, PR China.
authors.forename: Yong-Zhe
authors.initials: YZ
authors.lastname: Xiang
authors.forename: Yi-Le
authors.initials: YL
authors.lastname: Liao
authors.forename: Ji
authors.initials: J
authors.lastname: Zhang
authors.forename: Da-Wei
authors.initials: DW
authors.lastname: Zhang
authors.forename: Shan-Yong
authors.initials: SY
authors.lastname: Chen
authors.forename: Qiao-Sen
authors.initials: QS
authors.lastname: Lu
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhang
authors.forename: Hong-Hui
authors.initials: HH
authors.lastname: Lin
authors.forename: Xiao-Qi
authors.initials: XQ
authors.lastname: Yu
mesh_terms: D001372:Aza Compounds / Q000138:chemical synthesis* / Q000737:chemistry; D003516:Cycloparaffins / Q000138:chemical synthesis* / Q000737:chemistry; D053837:DNA Cleavage / Q000187:drug effects*; D004220:Disulfides / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry; D011073:Polyamines / Q000737:chemistry; D011108:Polymers / Q000138:chemical synthesis / Q000737:chemistry* / Q000378:metabolism; D044366:Transition Temperature
chemical_list: D001372:Aza Compounds; D003516:Cycloparaffins; D004220:Disulfides; D005456:Fluorescent Dyes; D011073:Polyamines; D011108:Polymers; C542857:poly(1,7-bis(mercaptoacetyl)-1,4,7,10-tetraazacyclododecane)