[Antioxidative and cytotoxic properties of diarylheptanoids isolated from Zingiber officinale].
OBJECTIVE
To investigate the antioxidant and cytotoxic properties of five diarylheptanoids (1-5) isolated from the rhizomes of Zingiber officinale.


METHODS
Various models such as scavenging superoxide anions and 1,1-diphenyl-2- picrylhydrazyl (DPPH) radicals, inhibiting lipid peroxidation, as well as protecting of rat pheochromocytoma (PC12) cells induced by hydrogen peroxide (H2O2) were employed to assay the antioxidative effects of the diarylheptanoids. The cytotoxicities of compounds 1-5 were measured with MTT assays.


RESULTS
The test compounds (1-5) showed promising DPPH inhibitory activities, and compound 5 exhibited the strongest DPPH scavenging activity with an IC50 value of (22.6+/-2.4) micromol x L(-1). Compounds 1, 3 and 4 showed potential anti-peroxidative effects with inhibitory rates of (66.3+/-15.4)%, (68.7+/-15.8)% and (72.2+/-10.6)%, respectively, at 100 microg x mL(-1). It could be observed that compounds 1, 3 and 4 demonstrated significant neuroprotective activities in a dose-dependent manner. Moreover, compound 3 exhibited certain cytotoxicities against human chronic myelogenous leukemia cells (K562) and its adriamycin-resistant cells (K562/ADR) with IC50 values of (34.9+/-0.6), (50.6+/-23.5) micromol x L(-1), respectively.


CONCLUSIONS
In vitro results demonstrated that five diarylheptanoids (1-5) isolated from the roots of Z. officinale were capable of scavenging radicals, inhibiting lipid peroxidation and protecting PC12 cells against the insult by H2O2. Additionally, compound 3 could inhibit the growth of K562 and K562/ADR cells.
Yang
Leixiang
L
School of Pharmacy, Wenzhou Medical College, Wenzhou 325035, China.
Zhou
Changxin
C
Huang
Kexin
K
Song
Liyan
L
Zheng
Qunxiong
Q
Yu
Rongmin
R
Zhang
Rongping
R
Wu
Yihang
Y
Zeng
Su
S
Cheng
Christopher H K
CH
Zhao
Yu
Y
Li
Xiaokun
X
Qu
Jia
J
D000818:Animals; D000975:Antioxidants / Q000633:toxicity*; D049109:Cell Proliferation / Q000187:drug effects; D003603:Cytotoxins / Q000633:toxicity*; D036381:Diarylheptanoids / Q000302:isolation & purification / Q000378:metabolism / Q000633:toxicity*; D005609:Free Radicals / Q000378:metabolism; D020939:Zingiber officinale / Q000737:chemistry*; D006801:Humans; D006861:Hydrogen Peroxide / Q000378:metabolism; D020014:K562 Cells; D009822:Oils, Volatile / Q000494:pharmacology; D016716:PC12 Cells; D051381:Rats; D017207:Rats, Sprague-Dawley
D000975:Antioxidants; D003603:Cytotoxins; D036381:Diarylheptanoids; D005609:Free Radicals; D009822:Oils, Volatile; D006861:Hydrogen Peroxide