The effect of levocabastine hydrochloride on human Tenon's capsule fibroblasts: inhibition of proliferation, suppression of DNA synthesis and induction of apoptosis.
OBJECTIVE
To investigate the effects of levocabastine hydrochloride on the profileration, Deoxyribonucleic acid (DNA) synthesis and induction of apoptosis, in human Tenon's capsule fibroblasts.


METHODS
The maximum intoxicate concentration was determined by measuring the release of lactate dehydrogenase. Proliferation of fibroblasts was analyzed with a WST-1 method. DNA synthesis was examined by quantifying the incorporation of 5-bromo-2'-deoxyuridine into dividing cells, and apoptosis was analyzed using an Annexin-V-FLUOS Staining Kit.


RESULTS
Levocabastine hydrochloride (10(-4) M), ketotifen fumarate (3 x 10(-5) M) and tranilast (10(-4) M) significantly inhibited proliferation of fibroblasts. Levocabastine hydrochloride (10(-4) M), ketotifen fumarate (10(-4) M) and tranilast (10(-3) M) inhibited DNA synthesis and induced apoptosis.


CONCLUSIONS
Levocabastine hydrochloride inhibited the proliferation of fibroblasts.
Asaoka
Ryo
R
Department of Ophthalmology, Hamamatsu University School of Medicine, Handayama, Hamamatsu city, Japan. Ryo.Asaoka@moorfields.nhs.uk
Nakagamil
Tetsushi
T
Zhu
Haogang
H
Liu
Yan
Y
Hotta
Yoshihiro
Y
D000368:Aged; D017209:Apoptosis / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D003228:Conjunctiva / Q000166:cytology / Q000187:drug effects*; D004247:DNA / Q000096:biosynthesis*; D004305:Dose-Response Relationship, Drug; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D039563:Histamine H1 Antagonists, Non-Sedating / Q000494:pharmacology*; D006801:Humans; D007665:Ketotifen / Q000494:pharmacology; D008297:Male; D010880:Piperidines / Q000494:pharmacology*; D062367:ortho-Aminobenzoates / Q000494:pharmacology
D039563:Histamine H1 Antagonists, Non-Sedating; D010880:Piperidines; D062367:ortho-Aminobenzoates; D004247:DNA; C047340:levocabastine; C012293:tranilast; D007665:Ketotifen