A cell-based screening system for detection of inhibitors toward mycobacterial cell wall core.
Mycobacterium tuberculosis and nonpathogenic bacteria, Corynebacterium glutamicum, possess a common and unusual cell wall architecture. A cell-based screening system was designed to identify novel compounds interacting with the synthesis, assembly or regulation of the M. tuberculosis cell wall. C. glutamicum was tested in a paired medium assay in 96-well plates with natural product extracts and pure chemical compounds in the presence and absence of the osmotic stabilizer, sorbitol and some ions. Growth was visually examined over a 12-h period and detected with a microplate reader for absorbance at 544 nm. Screening hits from the osmotic stabilizer rescue were then examined by mycolic acid analysis to confirm the effect on cell wall integrity.
Gao
Peng
P
Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing, China.
Guan
Yan
Y
Song
Danqing
D
Xiao
Chunling
C
D000904:Antibiotics, Antitubercular / Q000494:pharmacology; D002473:Cell Wall / Q000187:drug effects* / Q000648:ultrastructure*; D002855:Chromatography, Thin Layer; D048230:Corynebacterium glutamicum / Q000187:drug effects / Q000648:ultrastructure; D004232:Diuretics / Q000494:pharmacology; D004353:Drug Evaluation, Preclinical / Q000379:methods; D008826:Microbial Sensitivity Tests; D009169:Mycobacterium tuberculosis / Q000187:drug effects* / Q000648:ultrastructure*; D009171:Mycolic Acids / Q000032:analysis; D013012:Sorbitol / Q000494:pharmacology; D013056:Spectrophotometry, Ultraviolet
D000904:Antibiotics, Antitubercular; D004232:Diuretics; D009171:Mycolic Acids; D013012:Sorbitol