Effects of the NADPH oxidase p22phox C242T polymorphism on endurance exercise performance and oxidative DNA damage in response to aerobic exercise training.
We examined the effects of the NADPH oxidase p22phox C242T polymorphism on endurance exercise performance and oxidative DNA damage in response to acute and chronic exercises. One hundred three subjects were recruited, among which 26 healthy subjects (CC: 12, TC: 12, and TT: 2) were studied during rest, exercise at 85% VO(2)max, and recovery before and after 8 weeks of tread-mill running. Lymphocyte DNA damage increased significantly in response to exercise (p < 0.05). There were no significant differences in plasma MDA, SOD concentrations and lymphocyte DNA damage between CC genotype and T allele group, but significant endurance training differences were observed. Endurance training increased exercise time to exhaustion in both the CC genotype and T allele groups (p < 0.05) but no significant difference was found between groups. The results of the current study with young, healthy, Korean men are interpreted to mean that 1) the majority had the CC genotype of the NADPH oxidase p22phox C242T polymorphism (82.5%: CC, 15.5%: TC, 1.9%: TT), 2) acute exercise increased lymphocyte DNA damage, 3) endurance training significantly increased exercise time to exhaustion, and alleviated lymphocyte DNA damage, and 4) The NADPH oxidase p22phox C242T polymorphism, however, did not alter lymphocyte DNA damage or exercise performance at rest, immediately after exercise, or during recovery.
Paik
Il-Young
IY
Exercise Physiology Laboratory, Department of Physical Education, Yonsei University, Seoul 120-749, Korea.
Jin
Chan-Ho
CH
Jin
Hwa-Eun
HE
Kim
Young-Il
YI
Cho
Su-Youn
SY
Roh
Hee-Tae
HT
Suh
Ah-Ram
AR
Suh
Sang-Hoon
SH
D000328:Adult; D020552:Comet Assay; D004249:DNA Damage / Q000276:immunology; D004252:DNA Mutational Analysis; D015444:Exercise / Q000502:physiology*; D005080:Exercise Test; D005500:Follow-Up Studies; D006801:Humans; D008214:Lymphocytes / Q000502:physiology*; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D019255:NADPH Oxidases / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D018384:Oxidative Stress / Q000502:physiology*; D010807:Physical Endurance / Q000502:physiology*; D011110:Polymorphism, Genetic; D013482:Superoxide Dismutase / Q000378:metabolism
D008315:Malondialdehyde; D013482:Superoxide Dismutase; D019255:NADPH Oxidases; C086986:CYBA protein, human