title: MHC class II-dependent basophil-CD4+ T cell interactions promote T(H)2 cytokine-dependent immunity.
abstract: Dendritic cells can prime naive CD4+ T cells; however, here we demonstrate that dendritic cell-mediated priming was insufficient for the development of T helper type 2 cell-dependent immunity. We identify basophils as a dominant cell population that coexpressed major histocompatibility complex class II and interleukin 4 message after helminth infection. Basophilia was promoted by thymic stromal lymphopoietin, and depletion of basophils impaired immunity to helminth infection. Basophils promoted antigen-specific CD4+ T cell proliferation and interleukin 4 production in vitro, and transfer of basophils augmented the population expansion of helminth-responsive CD4+ T cells in vivo. Collectively, our studies suggest that major histocompatibility complex class II-dependent interactions between basophils and CD4+ T cells promote T helper type 2 cytokine responses and immunity to helminth infection.
authors.affiliation: Department of Pathobiology, University of Pennsylvania, Philadelphia, Pennsylvania, USA.
authors.forename: Jacqueline G
authors.initials: JG
authors.lastname: Perrigoue
authors.forename: Steven A
authors.initials: SA
authors.lastname: Saenz
authors.forename: Mark C
authors.initials: MC
authors.lastname: Siracusa
authors.forename: Eric J
authors.initials: EJ
authors.lastname: Allenspach
authors.forename: Betsy C
authors.initials: BC
authors.lastname: Taylor
authors.forename: Paul R
authors.initials: PR
authors.lastname: Giacomin
authors.forename: Meera G
authors.initials: MG
authors.lastname: Nair
authors.forename: Yurong
authors.initials: Y
authors.lastname: Du
authors.forename: Colby
authors.initials: C
authors.lastname: Zaph
authors.forename: Nico
authors.initials: N
authors.lastname: van Rooijen
authors.forename: Michael R
authors.initials: MR
authors.lastname: Comeau
authors.forename: Edward J
authors.initials: EJ
authors.lastname: Pearce
authors.forename: Terri M
authors.initials: TM
authors.lastname: Laufer
authors.forename: David
authors.initials: D
authors.lastname: Artis
mesh_terms: D000818:Animals; D001491:Basophils / Q000166:cytology / Q000276:immunology* / Q000378:metabolism; D039521:CD11c Antigen / Q000276:immunology; D015496:CD4-Positive T-Lymphocytes / Q000166:cytology / Q000276:immunology* / Q000378:metabolism; D002450:Cell Communication / Q000276:immunology; D049109:Cell Proliferation; D016207:Cytokines / Q000276:immunology* / Q000378:metabolism; D005260:Female; D005434:Flow Cytometry; D015870:Gene Expression; D000949:Histocompatibility Antigens Class II / Q000235:genetics / Q000276:immunology*; D007109:Immunity / Q000276:immunology*; D015151:Immunoblotting; D015847:Interleukin-4 / Q000235:genetics / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008815:Mice, Inbred Strains; D018345:Mice, Knockout; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012555:Schistosomiasis mansoni / Q000276:immunology / Q000469:parasitology; D018418:Th2 Cells / Q000378:metabolism; D013950:Thymus Gland / Q000166:cytology / Q000276:immunology; D014257:Trichuriasis / Q000276:immunology / Q000469:parasitology
chemical_list: D039521:CD11c Antigen; D016207:Cytokines; D000949:Histocompatibility Antigens Class II; D015847:Interleukin-4