title: Oleate hydratase catalyzes the hydration of a nonactivated carbon-carbon bond.
abstract: The hydration of oleic acid into 10-hydroxystearic acid was originally described for a Pseudomonas cell extract almost half a century ago. In the intervening years, the enzyme has never been characterized in any detail. We report here the isolation and characterization of oleate hydratase (EC 4.2.1.53) from Elizabethkingia meningoseptica.
authors.affiliation: Department of Biotechnology, Delft University of Technology, Delft, The Netherlands.
authors.forename: Loes E
authors.initials: LE
authors.lastname: Bevers
authors.forename: Martijn W H
authors.initials: MW
authors.lastname: Pinkse
authors.forename: Peter D E M
authors.initials: PD
authors.lastname: Verhaert
authors.forename: Wilfred R
authors.initials: WR
authors.lastname: Hagen
mesh_terms: D019840:2-Propanol / Q000494:pharmacology; D001426:Bacterial Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D004789:Enzyme Activation / Q000187:drug effects; D041965:Flavobacteriaceae / Q000201:enzymology*; D006836:Hydro-Lyases / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D008969:Molecular Sequence Data; D009829:Oleic Acids / Q000737:chemistry / Q000378:metabolism*; D012997:Solvents / Q000494:pharmacology; D013229:Stearic Acids / Q000737:chemistry / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D009829:Oleic Acids; D012997:Solvents; D013229:Stearic Acids; C070376:10-hydroxystearic acid; D006836:Hydro-Lyases; D019840:2-Propanol