title: Piercing and vacuum infiltration of the mature embryo: a simplified method for Agrobacterium-mediated transformation of indica rice.
abstract: Traditional transformation methods are complex and time consuming. It is generally difficult to transform indica rice varieties using traditional transformation methods due to their poor regeneration. In this contribution, a simple method was developed for the transformation of indica rice. In this method, the mature embryos of soaked seeds were pierced by a needle, and then soaked in the Agrobacterium inoculum under vacuum infiltration. The inoculated seeds germinated and grew to maturation (T (0)) under nonsterile conditions. The herbicide or antibiotic analysis and molecular analysis were conducted on T (0) plants. The results showed that although the efficiency of transformation was about 6.0%, it was easier to transform indica rice using the proposed method, and the transformation process was significantly shortened. The success of transformation was further confirmed by the genetic and molecular analyses of T (1) transformants.
authors.affiliation: Institute of Bioscience and Biotechnology, Hunan University, Changsha, 410082, Hunan, China.
authors.forename: Jianzhong
authors.initials: J
authors.lastname: Lin
authors.forename: Bo
authors.initials: B
authors.lastname: Zhou
authors.forename: Yuanzhu
authors.initials: Y
authors.lastname: Yang
authors.forename: Jin
authors.initials: J
authors.lastname: Mei
authors.forename: Xiaoying
authors.initials: X
authors.lastname: Zhao
authors.forename: Xinhong
authors.initials: X
authors.lastname: Guo
authors.forename: Xingqun
authors.initials: X
authors.lastname: Huang
authors.forename: Dongying
authors.initials: D
authors.lastname: Tang
authors.forename: Xuanming
authors.initials: X
authors.lastname: Liu
mesh_terms: D018744:DNA, Plant / Q000235:genetics; D018506:Gene Expression Regulation, Plant; D018014:Gene Transfer Techniques; D012275:Oryza / Q000235:genetics*; D030821:Plants, Genetically Modified / Q000235:genetics; D012231:Rhizobium / Q000235:genetics*; D012639:Seeds / Q000235:genetics*; D014170:Transformation, Genetic; D014618:Vacuum
chemical_list: D018744:DNA, Plant