Off-line FIA monitoring of D-sorbitol consumption during L-sorbose production using a sorbitol biosensor.
A ferricyanide mediated amperometric biosensor system implementing D-sorbitol dehydrogenase together with diaphorase for sensitive detection of D-sorbitol was used. The biosensor system was successfully integrated into an off-line FIA system with a throughput of detection of 10 h(-1). The device exhibited limit of detection of 20 microM with an average relative standard deviation of analysis of samples of 2.2%. The signal of the biosensor was linear up to 1.1 mM for D-sorbitol with sensitivity of (72 +/- 2) nA mM(-1), while a dynamic range was much wider up to 18 mM. The sorbitol biosensor gave reliable results even in the presence of a high molar excess of L-sorbose, a product of the biotransformation process, as judged from an excellent agreement with HPLC and GC.
Sefcovicová
Jana
J
Department of Glycobiotechnology, Center for Glycomics, Institute of Chemistry, Slovak Academy of Sciences, Dubravska cesta 9, SK-845 38 Bratislava, Slovakia.
Vikartovská
Alica
A
Pätoprstý
Vladimír
V
Magdolen
Peter
P
Katrlík
Jaroslav
J
Tkac
Jan
J
Gemeiner
Peter
P
D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D001711:Biotransformation; D004566:Electrodes; D005292:Ferricyanides / Q000737:chemistry; D017022:Flow Injection Analysis; D007064:L-Iditol 2-Dehydrogenase / Q000378:metabolism; D013012:Sorbitol / Q000032:analysis* / Q000378:metabolism; D013013:Sorbose / Q000378:metabolism*
D005292:Ferricyanides; C007931:hexacyanoferrate III; D013012:Sorbitol; D007064:L-Iditol 2-Dehydrogenase; D013013:Sorbose