A substrate channel in the nitrogenase MoFe protein.
Nitrogenase catalyzes the six electron/six proton reduction of N2 to two ammonia molecules at a complex organometallocluster called "FeMo cofactor." This cofactor is buried within the alpha-subunit of the MoFe protein, with no obvious access for substrates. Examination of high-resolution X-ray crystal structures of MoFe proteins from several organisms has revealed the existence of a water-filled channel that extends from the solvent-exposed surface to a specific face of FeMo cofactor. This channel could provide a pathway for substrate and product access to the active site. In the present work, we examine this possibility by substituting four different amino acids that line the channel with other residues and analyze the impact of these substitutions on substrate reduction kinetic parameters. Each of the MoFe protein variants was purified and kinetic parameters were established for the reduction of the substrates N2, acetylene, azide, and propyne. For each MoFe protein, V (max) values for the different substrates were found to be nearly unchanged when compared with the values for the wild-type MoFe protein, indicating that electron delivery to the active site is not compromised by the various substitutions. In contrast, the K(m) values for these substrates were found to increase significantly (up to 22-fold) in some of the MoFe protein variants compared with the wild-type MoFe protein values. Given that each of the amino acids that were substituted is remote from the active site, these results are consistent with the water-filled channel functioning as a substrate channel in the MoFe protein.
Barney
Brett M
BM
Department of Chemistry and Biochemistry, Utah State University, 0300 Old Main Hill, Logan, UT 84322, USA.
Yurth
Michael G
MG
Dos Santos
Patricia C
PC
Dean
Dennis R
DR
Seefeldt
Lance C
LC
D016948:Azotobacter vinelandii / Q000201:enzymology* / Q000235:genetics; D020134:Catalytic Domain; D003124:Colorimetry; D004578:Electron Spin Resonance Spectroscopy; D005816:Genetic Complementation Test; D007700:Kinetics; D008958:Models, Molecular; D008982:Molybdenum / Q000032:analysis; D008983:Molybdoferredoxin / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D016297:Mutagenesis, Site-Directed; D050505:Mutant Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D009591:Nitrogenase / Q000737:chemistry* / Q000378:metabolism; D012984:Software; D013379:Substrate Specificity / Q000235:genetics
D008983:Molybdoferredoxin; D050505:Mutant Proteins; D008982:Molybdenum; D009591:Nitrogenase