[Establishment of hepatocyte extraction combined with HPLC(HE-HPLC) and application in analysis of active components in the fruits of Gardenia jasminoides extract].
To screen effective principles from traditional Chinese medicine, a method named hepatocyte extraction combined with HPLC (HE-HPLC) was establish in this study. The active principles in the fruits of Gardenia jasminoides Ellis extract were combined with the hepatocytes under imitated physiological environments. Then the unattached substances were washed off by PBS with pH 7.4. After that the conjugated compounds were eluted by PBS with pH 4.0. These compounds released from target sites were collected and handled through SPE to be condensed, and analyzed by HPLC. The results indicated that two characteristic active compounds in the fruits of G. jasminoides extract binded to the hepatocytes. One of them is geniposide. The other is continued to be identified. It is showed that active principles which could bind to hepotocyte (through receptors, Channels, enzymes, etc.) could be detected, at least partly, by HE-HPLC analysis. There was a significant correlation between the retention properties of the active compounds which was obtained by HE-HPLC and their pharmacological effects on hepotocytes.
Hong
Min
M
National Standard Laboratory of Traditional Chinese Medicine Pharmacology, Nanjing University of Chinese Medicine, Nanjing 210029, China. hongmin72@hotmail.com
Ma
Hongyu
H
Zhu
Quan
Q
D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004365:Drugs, Chinese Herbal / Q000032:analysis*; D005638:Fruit / Q000737:chemistry; D032062:Gardenia / Q000737:chemistry*; D022781:Hepatocytes / Q000187:drug effects* / Q000378:metabolism; D039823:Iridoids / Q000494:pharmacology*; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology*; D051381:Rats
D004365:Drugs, Chinese Herbal; D039823:Iridoids; D010936:Plant Extracts; C007835:geniposide