Vaccination with Streptococcus suis serotype 2 recombinant 6PGD protein provides protection against S. suis infection in swine.
Streptococcus suis serotype 2 (S. suis 2 or SS2) is the causative agent of several diseases in both pigs and humans. 6-Phosphogluconate dehydrogenase (6PGD) is a cell surface protein in SS2. In this study, the immunogenicity and protective efficacy of recombinant 6PGD (r6PGD) from SS2 was evaluated in piglets. Immunization with an r6PGD-containing adjuvant induced a vigorous immunoglobulin G (IgG) response, with the titer of IgG2 being higher than that of IgG1. Immunization of piglets with r6PGD yielded 50% survival upon an intravenous challenge with a lethal dose of SS2. Piglets immunized with the r6PDG vaccine were better protected than those immunized with the adjuvant control. The clinical signs and histopathological changes in the piglets were recorded. Collectively, these results suggest that r6PGD can confer partial protection against SS2 infection and could be useful for the development of subunit vaccines against SS2.
Tan
Chen
C
State Key Laboratory of Agricultural Microbiology, Division of Animal Pathogens, College of Animal Science and Veterinary Medicine, Huazhong Agricultural University, Wuhan, China.
Liu
Manli
M
Liu
Jinlin
J
Yuan
Fangyan
F
Fu
Shulin
S
Liu
Yuan
Y
Jin
Meilin
M
Bei
Weicheng
W
Chen
Huanchun
H
D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D001426:Bacterial Proteins / Q000235:genetics / Q000276:immunology; D001921:Brain / Q000382:microbiology / Q000473:pathology; D005954:Glucosephosphate Dehydrogenase / Q000235:genetics / Q000276:immunology*; D007074:Immunoglobulin G / Q000097:blood; D008168:Lung / Q000382:microbiology / Q000473:pathology; D013290:Streptococcal Infections / Q000276:immunology / Q000517:prevention & control / Q000662:veterinary*; D022541:Streptococcal Vaccines / Q000008:administration & dosage / Q000276:immunology*; D017011:Streptococcus suis / Q000276:immunology*; D016019:Survival Analysis; D013552:Swine; D013553:Swine Diseases / Q000276:immunology / Q000517:prevention & control*; D022223:Vaccines, Subunit / Q000235:genetics / Q000276:immunology; D014614:Vaccines, Synthetic / Q000235:genetics / Q000276:immunology
D000907:Antibodies, Bacterial; D001426:Bacterial Proteins; D007074:Immunoglobulin G; D022541:Streptococcal Vaccines; D022223:Vaccines, Subunit; D014614:Vaccines, Synthetic; D005954:Glucosephosphate Dehydrogenase