title: [Model of experimental nonalcoholic steatohepatitis from use of methionine and choline deficient diet].
abstract: BACKGROUND
  There are still many unknown aspects about nonalcoholic steatohepatitis, especially regarding its pathophysiology and pharmacological treatment. Thus, experimental models are important for a better understanding of this disease and the evaluation of the effects of drugs.
  
  
  OBJECTIVE
  To develop a model of experimental nonalcoholic steatohepatitis from use of methionine and choline deficient diet.
  
  
  METHODS
  Fifty Wistar male rats were studied. A methionine and choline deficient diet has been processed in a craft. A group of 40 animals received the deficient diet for 90 days, and a group of 10 rats (control group) received the standardized ration in the same period. After, the animals were killed by decapitation, and laparotomy was performed. Hepatectomy was performed and the liver was studied by macroscopy and microscopy. The level of significance considered was of 0,05.
  
  
  RESULTS
  The rats that received the deficient diet showed significant loss of weight with findings from malnutrition and all of them had at least some degree of macrovesicular steatosis. The diagnosis of nonalcoholic steatohepatitis was performed in 27 (70%) of the 39 rats that received this deficient diet (1 rat died during the study). None of the 10 rats that received the standardized diet had histological abnormalities.
  
  
  CONCLUSIONS
  The diet restricted in methionine and choline induced steatosis and steatohepatitis in an animal model with low cost.
authors.affiliation: Fundação Faculdade Federal de Ciências Médicas de Porto Alegre, RS. izamin@terra.com.br
authors.forename: Idilio
authors.initials: I
authors.lastname: Zamin
authors.forename: Angelo Alves de
authors.initials: AA
authors.lastname: Mattos
authors.forename: Angelo Zambam de
authors.initials: AZ
authors.lastname: Mattos
authors.forename: Eduardo
authors.initials: E
authors.lastname: Migon
authors.forename: Ernesto
authors.initials: E
authors.lastname: Soares
authors.forename: Marcos Luiz Santos
authors.initials: ML
authors.lastname: Perry
mesh_terms: D000821:Animal Feed / Q000009:adverse effects*; D000818:Animals; D002796:Choline Deficiency / Q000150:complications*; D004195:Disease Models, Animal; D005234:Fatty Liver / Q000209:etiology* / Q000473:pathology; D008099:Liver / Q000473:pathology; D008297:Male; D008715:Methionine / Q000172:deficiency*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D008715:Methionine