Regional distribution and the dynamics of n-decanoyl ghrelin, another acyl-form of ghrelin, upon fasting in rodents.
n-Decanoyl ghrelin (D-ghrelin), a member of ghrelin-derived peptides, is found in plasma and the stomach; however, there have so far been no studies describing its dynamics. A D-ghrelin-specific radioimmunoassay was established to examine the tissue distribution and the kinetics of D-ghrelin in mice. The effect of D-ghrelin on food intake was also examined and compared to n-octanoyl ghrelin (O-ghrelin). D-ghrelin was detected throughout the gastrointestinal tissue and plasma with highest level in the stomach. An immunofluorescent study revealed the co-localization of D- and O-ghrelin in the same stomach cells. Upon fasting, the levels of D-ghrelin in the stomach and plasma significantly increased, while that of O-ghrelin in the stomach declined. D-ghrelin increased the 2 h food consumption in mice as O-ghrelin does. These findings indicate that D-ghrelin is mainly produced in the stomach to work in concert with O-ghrelin. The different kinetics of D- and O-ghrelin in the stomach upon fasting implies the possibility of D-ghrelin-specific bioregulation.
Hiejima
Hiroshi
H
Department of Physiology, Kurume University School of Medicine, Kurume, 830-0011 Fukuoka, Japan.
Nishi
Yoshihiro
Y
Hosoda
Hiroshi
H
Yoh
Junko
J
Mifune
Hiroharu
H
Satou
Motoyasu
M
Sugimoto
Hiroyuki
H
Chiba
Seiichi
S
Kawahara
Yukie
Y
Tanaka
Eiichiro
E
Yoshimatsu
Hironobu
H
Uchimura
Naohisa
N
Kangawa
Kenji
K
Kojima
Masayasu
M
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D004435:Eating / Q000502:physiology; D005215:Fasting / Q000502:physiology*; D005455:Fluorescent Antibody Technique; D005753:Gastric Mucosa / Q000378:metabolism; D041981:Gastrointestinal Tract / Q000378:metabolism; D054439:Ghrelin / Q000097:blood* / Q000378:metabolism*; D007031:Hypothalamus / Q000378:metabolism; D066298:In Vitro Techniques; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D010179:Pancreas / Q000378:metabolism; D011863:Radioimmunoassay
D054439:Ghrelin