title: Immunization with inflammatory proteome of Brugia malayi adult worm induces a Th1/Th2-immune response and confers protection against the filarial infection.
abstract: Mastomys coucha and jirds (Meriones unguiculatus) were immunized with four cytokine-stimulating SDS-PAGE resolved fractions F5 (68-84 kDa), F6 (54-68 kDa), F10 (38-42 kDa) and F14 (20-28 kDa) of Brugia malayi adult worm to determine which of these fractions has the potential to influence the establishment of subsequently introduced B. malayi infection in the animals. The proteins in the fractions were analyzed by 2DE and MALDI-TOF. Immunization with F6 suppressed the establishment of third stage larva (L(3)) initiated infection in M. coucha (64%; P<0.01) and jird (42%; P<0.01). Survival of intraperitoneally implanted adult worms in M. coucha was lowered by F6 (72%; P<0.01) and F14 (66%; P<0.05) but not by F5 and F10. Immunization with F6 intensely upregulated both Th1 (IFN-gamma, TNF-alpha, IL-1 beta, IL-2, IL-6, IgG1, IgG2a and lymphoproliferation) and Th2 (IgG2b and IL-10) responses and NO release. Immunostimulatory proteins HSP60, intermediate filament protein, and translation elongation factor EF-2 were identified in F6 fraction by 2DE and MALDI. The findings suggest that F6 protects the host from the parasite via Th1/Th2 type responses and thus holds promise for development as a vaccine.
authors.affiliation: Division of Parasitology, Central Drug Research Institute, Lucknow 226001, India.
authors.forename: M K
authors.initials: MK
authors.lastname: Sahoo
authors.forename: B S
authors.initials: BS
authors.lastname: Sisodia
authors.forename: S
authors.initials: S
authors.lastname: Dixit
authors.forename: S K
authors.initials: SK
authors.lastname: Joseph
authors.forename: R L
authors.initials: RL
authors.lastname: Gaur
authors.forename: S K
authors.initials: SK
authors.lastname: Verma
authors.forename: A K
authors.initials: AK
authors.lastname: Verma
authors.forename: A K
authors.initials: AK
authors.lastname: Shasany
authors.forename: A A
authors.initials: AA
authors.lastname: Dowle
authors.forename: P Kalpana
authors.initials: PK
authors.lastname: Murthy
mesh_terms: D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood; D000947:Antigens, Helminth / Q000276:immunology* / Q000302:isolation & purification; D017178:Brugia malayi / Q000737:chemistry / Q000276:immunology*; D016207:Cytokines / Q000378:metabolism; D015180:Electrophoresis, Gel, Two-Dimensional; D005368:Filariasis / Q000517:prevention & control*; D005849:Gerbillinae; D015801:Helminth Proteins / Q000276:immunology* / Q000302:isolation & purification; D008297:Male; D013058:Mass Spectrometry; D051189:Murinae; D020543:Proteome / Q000032:analysis; D016019:Survival Analysis; D018417:Th1 Cells / Q000276:immunology*; D018418:Th2 Cells / Q000276:immunology*
chemical_list: D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D016207:Cytokines; D015801:Helminth Proteins; D020543:Proteome