title: Effect of acarbose on acute acidosis.
abstract: A challenge model was used to evaluate a new approach to controlling acute acidosis. Acute acidosis reduces performance in both dairy and beef cattle and most often occurs as a consequence of ingestion of large amounts of readily fermentable starch, resulting in increased production of volatile fatty acids (VFA) and lactic acid and a reduction in ruminal pH. Acarbose is an alpha-amylase and glucosidase inhibitor that slows the rate of degradation of starch to glucose, thereby reducing the rate of VFA production and maintaining rumen pH at a more stable level. It is commercially available (Glucobay, Bayer, Wuppertal, Germany) and indicated for the control of blood glucose in diabetic patients. The ability of acarbose to reduce the incidence of acidosis and the comparative efficacies of acarbose, sodium bicarbonate, and monensin were tested in 3 acute acidosis challenge experiments in cattle. Rumen-cannulated Holstein steers were challenged with a mixture of 48.4% cornstarch, 48.4% ground corn, 2.1% sodium caseinate, and 1.1% urea with or without test substance. The challenge was administered at a rate of 12.5 g/kg of body weight (BW) as a slurry through the cannula directly into the rumen. Ruminal pH was monitored at 10-min intervals throughout the study. Animals were removed from study and rumen contents replaced if they exhibited acute acidosis as defined as pH <4.5. If acidosis was not observed within 24 h, animals were subjected to a second challenge. Ruminal fluid samples were taken for measurement of VFA and lactate concentrations at various intervals after the challenge. In experiment 1, the carbohydrate challenge induced acidosis in 4 of 4 control animals and 0 of 4 animals treated with 2.14 or 21.4 mg of acarbose/kg of BW in the challenge based on the criterion of pH <4.5. In experiment 2, the carbohydrate challenge induced acidosis in 4 of 7 control animals and 1 of 7 animals when 1.07 mg of acarbose/kg of BW was included in the challenge. In experiment 3, acidosis was induced in 7 of 7 animals in the control, 1% sodium bicarbonate, and 12 mg of monensin/kg of dry matter intake groups and in 3 of 8 steers administered 1.07 mg of acarbose/kg of BW in the challenge. Increases in lactate concentrations and decreases in total VFA associated with acute acidosis were mitigated by acarbose. Thus, acarbose, an amylase and glucosidase inhibitor, prevented or reduced the incidence of acidosis in an acute challenge model in steers and was more effective than monensin or sodium bicarbonate.
authors.affiliation: Pfizer Animal Health, 7000 Portage Road, Kalamazoo, MI 49001, USA. carol.mclaughlin@pfizer.com
authors.forename: C L
authors.initials: CL
authors.lastname: McLaughlin
authors.forename: A
authors.initials: A
authors.lastname: Thompson
authors.forename: K
authors.initials: K
authors.lastname: Greenwood
authors.forename: J
authors.initials: J
authors.lastname: Sherington
authors.forename: C
authors.initials: C
authors.lastname: Bruce
mesh_terms: D020909:Acarbose / Q000494:pharmacology / Q000627:therapeutic use*; D000138:Acidosis / Q000188:drug therapy / Q000662:veterinary*; D000208:Acute Disease; D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000188:drug therapy*; D004040:Dietary Carbohydrates / Q000008:administration & dosage; D004435:Eating / Q000187:drug effects; D005232:Fatty Acids, Volatile / Q000032:analysis; D005766:Gastrointestinal Contents / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D007004:Hypoglycemic Agents / Q000494:pharmacology / Q000627:therapeutic use*; D019344:Lactic Acid / Q000032:analysis; D008297:Male; D011897:Random Allocation; D013997:Time Factors
chemical_list: D004040:Dietary Carbohydrates; D005232:Fatty Acids, Volatile; D007004:Hypoglycemic Agents; D019344:Lactic Acid; D020909:Acarbose