title: Design of a novel interleukin-13 antagonist from analysis of informational structure.
abstract: Interleukin-13 (IL-13) is one of the cytokines involved in the development of Th2-type immune response. It plays an important role in the pathogenesis of asthma and other allergic diseases. Two deletion forms of IL-13 were constructed on a basis of informational structure analysis and expressed in E. coli cells. They were found to differ in ability to stimulate proliferation of TF-1 cell line. Deletion variant 146 (DV146) completely lacks such activity, whereas DV148 provides about 50% of the proliferation stimulation. The simultaneous addition of DV146 with full-length IL-13 suppresses proliferation depending on the concentration of the deletion form. Thus, the designed protein acts as an antagonist of IL-13.
authors.affiliation: Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, 117997, Russia. alexei_nekrasov@mail.ru
authors.forename: A N
authors.initials: AN
authors.lastname: Nekrasov
authors.forename: L E
authors.initials: LE
authors.lastname: Petrovskaya
authors.forename: V A
authors.initials: VA
authors.lastname: Toporova
authors.forename: E A
authors.initials: EA
authors.lastname: Kryukova
authors.forename: A V
authors.initials: AV
authors.lastname: Rodina
authors.forename: E Yu
authors.initials: EY
authors.lastname: Moskaleva
authors.forename: M P
authors.initials: MP
authors.lastname: Kirpichnikov
mesh_terms: D000595:Amino Acid Sequence; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D015195:Drug Design; D006801:Humans; D018793:Interleukin-13 / Q000037:antagonists & inhibitors* / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D008968:Molecular Conformation; D008969:Molecular Sequence Data; D017384:Sequence Deletion
chemical_list: D018793:Interleukin-13