A toolkit for high-throughput, cross-species gene engineering in Drosophila.
We generated two complementary genomic fosmid libraries for Drosophila melanogaster and Drosophila pseudoobscura that permit seamless modification of large genomic clones by high-throughput recombineering and direct transgenesis. The fosmid transgenes recapitulated endogenous gene expression patterns. These libraries, in combination with recombineering technology, will be useful to rescue mutant phenotypes, allow imaging of gene products in living flies and enable systematic analysis and manipulation of gene activity across species.
Ejsmont
Radoslaw K
RK
Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany.
Sarov
Mihail
M
Winkler
Sylke
S
Lipinski
Kamil A
KA
Tomancak
Pavel
P
D000818:Animals; D030801:Animals, Genetically Modified / Q000235:genetics*; D002874:Chromosome Mapping / Q000379:methods*; D003001:Cloning, Molecular / Q000379:methods*; D004330:Drosophila / Q000235:genetics*; D015723:Gene Library; D005818:Genetic Engineering / Q000379:methods*