title: Chromosomal homology between the human and the bovine DMRT1 genes.
abstract: Doublesex and mab-3 related transcription factor 1 (DMRT1) is considered to be the most conserved gene among loci involved in the molecular pathways of animal sexual development. In the majority of the extensively examined vertebrates, its function is limited to the upstream or downstream testis regulators acting during embryogenesis. Our present study demonstrated the structural homology between DMRT1 orthologos in human and cattle. A BAC clone with a specific bovine sequence of the gene was used in the FISH mapping experiments. The physical localization of DMRT1 in cattle (BTA 8q17) was determined and its homology to the human locus was shown (HSA 9p24.3). Furthermore, another BAC probe, containing the sequence of the human homologue (pBACe3.6), generated hybridisation signals on bovine metaphase chromosomes and indicated the physical location of the autosomal bovine DMRT1 locus. Further investigations of the gene in domestic animals might provide more support for its conservative status and may help in understanding the molecular mechanisms involved in the occurrence of sexual abnormalities often diagnosed in livestock.
authors.affiliation: Department of Immuno- and Cytogenetics of Animals, National Research Institute of Animal Production, Krakowska 1, 32-083 Balice n. Kraków, Poland. abratus@izoo.krakow.pl
authors.forename: Anna
authors.initials: A
authors.lastname: Bratuś
authors.forename: Monika
authors.initials: M
authors.lastname: Bugno
authors.forename: Jolanta
authors.initials: J
authors.lastname: Klukowska-Rötzler
authors.forename: Małgorzata
authors.initials: M
authors.lastname: Sawińska
authors.forename: Andre
authors.initials: A
authors.lastname: Eggen
authors.forename: Ewa
authors.initials: E
authors.lastname: Słota
mesh_terms: D000818:Animals; D002417:Cattle / Q000235:genetics*; D002874:Chromosome Mapping; D017124:Conserved Sequence; D004247:DNA / Q000235:genetics; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008297:Male; D014157:Transcription Factors / Q000235:genetics*
chemical_list: C110547:DMRT1 protein; D014157:Transcription Factors; D004247:DNA