title: Glabridin inhibits lipopolysaccharide-induced activation of a microglial cell line, BV-2, by blocking NF-kappaB and AP-1.
abstract: Glabridin, a flavonoid present in licorice root, is known to have antiinflammatory and cardiovascular protective activities. The present study reports an inhibitory effect of glabridin on microglial activation. Glabridin dose-dependently attenuated lipopolysaccharide (LPS)-induced production of inflammatory mediators, including nitric oxide, tumor necrosis factor-alpha and interleukin-1beta, in BV-2 cells, a murine microglia cell line. Moreover, mRNA expression of these inflammatory mediators was also suppressed by glabridin in LPS-stimulated BV-2 cells. Further study demonstrated that glabridin inhibited LPS-induced DNA binding activity of NF-kappaB and AP-1 in BV-2 cells. Collectively, the results presented in this report demonstrate that glabridin inhibits the production of inflammatory mediators in BV-2 cells and this is mediated, at least in part, by blocking NF-kappaB and AP-1 activation. The results suggest that glabridin might be a potential therapeutic agent for the treatment of neuroinflammatory and neurodegenerative diseases.
authors.affiliation: Collage of Pharmacy, Chungnam National University, Daejeon, Korea.
authors.forename: Sun Hong
authors.initials: SH
authors.lastname: Park
authors.forename: Jong Soon
authors.initials: JS
authors.lastname: Kang
authors.forename: Yeo Dae
authors.initials: YD
authors.lastname: Yoon
authors.forename: Kiho
authors.initials: K
authors.lastname: Lee
authors.forename: Kang-Jeon
authors.initials: KJ
authors.lastname: Kim
authors.forename: Ki Hoon
authors.initials: KH
authors.lastname: Lee
authors.forename: Chang Woo
authors.initials: CW
authors.lastname: Lee
authors.forename: Eun-Yi
authors.initials: EY
authors.lastname: Moon
authors.forename: Sang-Bae
authors.initials: SB
authors.lastname: Han
authors.forename: Bong Hee
authors.initials: BH
authors.lastname: Kim
authors.forename: Hwan Mook
authors.initials: HM
authors.lastname: Kim
authors.forename: Song-Kyu
authors.initials: SK
authors.lastname: Park
mesh_terms: D000818:Animals; D002460:Cell Line; D053583:Interleukin-1beta / Q000378:metabolism; D007529:Isoflavones / Q000494:pharmacology*; D008070:Lipopolysaccharides; D051379:Mice; D017628:Microglia / Q000187:drug effects* / Q000378:metabolism; D016328:NF-kappa B / Q000037:antagonists & inhibitors*; D009569:Nitric Oxide / Q000378:metabolism; D010636:Phenols / Q000494:pharmacology*; D018808:Transcription Factor AP-1 / Q000037:antagonists & inhibitors*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D053583:Interleukin-1beta; D007529:Isoflavones; D008070:Lipopolysaccharides; D016328:NF-kappa B; D010636:Phenols; D018808:Transcription Factor AP-1; D014409:Tumor Necrosis Factor-alpha; D009569:Nitric Oxide; C107601:glabridin