LeY oligosaccharide upregulates DAG/PKC signaling pathway in the human endometrial cells.
LeY oligosaccharide is stage specifically expressed by the embryo and uterine endometrium, and it plays important roles in embryo implantation. In addition to participating in the recognition and adhesion on fetal-maternal interface, LeY potentially regulates the expression of some implantation-related factors. However, it remains elusive whether it can mediate the involved signaling pathway. In this study, agarose-LeY beads were used to mimic the embryos, and the effects of LeY oligosaccharide on DAG/PKC signaling pathway was studied in human endometrial epithelial cells. Results showed that LeY could significantly trigger the activation of cPKCalpha and cPKCbeta2, and their translocation from the cytosol to the plasma membrane. The cellular DAG content was also upregulated, and the activation of PLCgamma1 was promoted. On the contrary, DAG/PKC signaling pathway was significantly inhibited when anti-LeY antibody was used after confirmation of LeY expression in human endometrial epithelial cells by immunohistochemistry and flow cytometry. These results suggest that LeY oligosaccharide acts as a signal molecule to modulate DAG/PKC signaling pathway.
Li
Yali
Y
Department of Biochemistry and Molecular Biology, Liaoning Provincial Core Lab of Glycobiology and Glycoengineering, Dalian Medical University, Dalian, 116044, People's Republic of China.
Ma
Keli
K
Sun
Ping
P
Liu
Shuai
S
Qin
Huamin
H
Zhu
Zhengmei
Z
Wang
Xiaoqi
X
Yan
Qiu
Q
D002460:Cell Line; D004075:Diglycerides / Q000378:metabolism*; D004717:Endometrium / Q000166:cytology* / Q000201:enzymology*; D004847:Epithelial Cells / Q000166:cytology / Q000201:enzymology; D005260:Female; D006801:Humans; D007983:Lewis Blood Group Antigens / Q000378:metabolism*; D009844:Oligosaccharides / Q000378:metabolism; D051966:Phospholipase C gamma / Q000378:metabolism; D010766:Phosphorylation; D011493:Protein Kinase C / Q000378:metabolism*; D021381:Protein Transport; D015398:Signal Transduction; D015854:Up-Regulation
D004075:Diglycerides; D007983:Lewis Blood Group Antigens; C094162:Lewis Y antigen; D009844:Oligosaccharides; D011493:Protein Kinase C; D051966:Phospholipase C gamma