A highly sensitive and robust UPLC-MS with electrospray ionization method for quantitation of taxifolin in rat plasma.
A sensitive ultra performance liquid chromatography-mass spectrometry method has been developed and validated for the quantification of taxifolin in rat plasma. Following liquid/liquid extraction by ethyl acetate, the analytes were separated on a Sunfire (2.1 mm x 50 mm, 3.5 microm) column and analyzed in the selected ion recording with a negative electrospray ionization mode. The method was linear over the concentration range of 6-6750 ng/mL. Intra- and inter-day precisions were all within 8% and accuracy ranged from 92.9% to 105.1%. The lower limit of quantification was 6 ng/mL. The present method was successfully applied to the estimation of the pharmacokinetic parameters of taxifolin following intravenous and oral administration to rats. The absolute bioavailability of taxifolin was 0.17% in rat.
Wang
Xiaodan
X
College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058, Zhejiang Province, PR China.
Xia
Hongjun
H
Xing
Feng
F
Deng
Guifeng
G
Shen
Qi
Q
Zeng
Su
S
D000818:Animals; D002853:Chromatography, Liquid / Q000379:methods*; D011794:Quercetin / Q000031:analogs & derivatives* / Q000097:blood / Q000493:pharmacokinetics; D051381:Rats; D012680:Sensitivity and Specificity; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
D011794:Quercetin; C003377:taxifolin