Determination of ethylglucuronide in oral fluid by ultra-performance liquid chromatography- tandem mass spectrometry.
An ultra-performance liquid chromatography-tandem mass spectrometry method has been developed and validated for the determination of ethylglucuronide (EtG) in oral fluid. Sample clean-up was achieved by solid-phase extraction with a Hyper-SEP SAX column. Negative ionization was performed in the multiple reaction monitoring mode. Two transitions were monitored for the analyte and one for the internal standard EtG-d(5). The calibration range was 4.4-222 ng/mL. The recovery of the analyte ranged from 86 to 99%, and the between-assay precisions ranged from 5 to 9% RSD. The limit of quantification was found to be 4.4 ng/mL. The concentration of EtG in oral fluid collected 2-14 h after a moderate alcohol intake varied from 13.3 to 57.7 ng/mL.
Hegstad
S
S
Norwegian Institute of Public Health, Division of Forensic Toxicology and Drug Abuse, Nydalen, NO-0403 Oslo, Norway. solfrid.hegstad@stoalv.no
Johnsen
L
L
Mørland
J
J
Christophersen
A S
AS
D000428:Alcohol Drinking / Q000378:metabolism*; D001711:Biotransformation; D002138:Calibration; D002853:Chromatography, Liquid / Q000592:standards; D000431:Ethanol / Q000378:metabolism*; D005965:Glucuronates / Q000378:metabolism*; D006801:Humans; D015203:Reproducibility of Results; D012463:Saliva / Q000378:metabolism*; D015813:Substance Abuse Detection / Q000379:methods* / Q000592:standards; D053719:Tandem Mass Spectrometry / Q000592:standards; D013997:Time Factors
D005965:Glucuronates; C093924:ethyl glucuronide; D000431:Ethanol