Molecular adaptation of sourdough Lactobacillus plantarum DC400 under co-cultivation with other lactobacilli.
This work was aimed at investigating the molecular mechanisms of Quorum Sensing (QS) in Lactobacillus plantarum DC400 when co-cultured with other sourdough lactobacilli. The growth and survival of L. plantarum DC400 was not affected when co-cultivated with Lactobacillus sanfranciscensis DPPMA174 or Lactobacillus rossiae A7. Nevertheless, 2-DE analysis showed that the level of protein expression of L. plantarum DC400 increased under co-culture conditions. Although several proteins were commonly induced in both co-cultures, the highest induction was found in co-culture with L. rossiae A7. Overexpressed proteins, related to QS and stress response mechanisms, were identified: DnaK, GroEL, 30S ribosomal protein S1 and S6, ATP synthase subunit beta, adenosylmethionine synthetase (MetK), phosphopyruvate hydratase, phosphoglycerate kinase, elongation factor Tu, putative manganese-dependent inorganic pyrophosphatase, d-lactate dehydrogenase, triosephosphate isomerase, fructose-bisphosphate aldolase and nucleoside-diphosphate kinase. As shown by real-time PCR, expression of the luxS gene of L. plantarum DC400 was also affected during co-cultivation. According to overexpression of MetK and luxS during co-cultivation, synthesis of AI-2-like substances was also influenced by the type of microbial co-cultures. This study showed that expression of some genes/proteins, also QS-related, in L. plantarum was influenced by co-cultivation of other sourdough lactobacilli.
Di Cagno
Raffaella
R
Dipartimento di Protezione delle Piante e Microbiologia Applicata, Università degli Studi di Bari, Italy.
De Angelis
Maria
M
Coda
Rossana
R
Minervini
Fabio
F
Gobbetti
Marco
M
D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D015180:Electrophoresis, Gel, Two-Dimensional; D005516:Food Microbiology; D015964:Gene Expression Regulation, Bacterial; D007778:Lactobacillus / Q000737:chemistry / Q000201:enzymology / Q000235:genetics / Q000502:physiology*; D048191:Lactiplantibacillus plantarum / Q000737:chemistry / Q000201:enzymology / Q000235:genetics / Q000502:physiology*; D050296:Microbial Viability; D008969:Molecular Sequence Data; D053038:Quorum Sensing
D001426:Bacterial Proteins