title: A facile method for reversibly linking a recombinant protein to DNA.
abstract: We present a facile method for linking recombinant proteins to DNA. It is based on the nickel-mediated interaction between a hexahistidine tag (His(6)-tag) and DNA functionalized with three nitrilotriacetic acid (NTA) groups. The resulting DNA-protein linkage is site-specific. It can be broken quickly and controllably by the addition of a chelating agent that binds nickel. We have used this new linker to bind proteins to a variety of DNA motifs commonly used in the fabrication of nanostructures by DNA self-assembly.
authors.affiliation: Department of Physics, Clarendon Laboratory, University of Oxford, Parks Road, Oxford OX13PU, UK.
authors.forename: Russell P
authors.initials: RP
authors.lastname: Goodman
authors.forename: Christoph M
authors.initials: CM
authors.lastname: Erben
authors.forename: Jonathan
authors.initials: J
authors.lastname: Malo
authors.forename: Wei M
authors.initials: WM
authors.lastname: Ho
authors.forename: Mireya L
authors.initials: ML
authors.lastname: McKee
authors.forename: Achillefs N
authors.initials: AN
authors.lastname: Kapanidis
authors.forename: Andrew J
authors.initials: AJ
authors.lastname: Turberfield
mesh_terms: D004247:DNA / Q000737:chemistry*; D006639:Histidine / Q000737:chemistry; D009532:Nickel / Q000737:chemistry; D009571:Nitrilotriacetic Acid / Q000737:chemistry; D020411:Oligonucleotide Array Sequence Analysis; D009841:Oligonucleotides / Q000737:chemistry; D009842:Oligopeptides / Q000737:chemistry; D011485:Protein Binding; D011994:Recombinant Proteins / Q000737:chemistry*
chemical_list: C471213:His-His-His-His-His-His; D009841:Oligonucleotides; D009842:Oligopeptides; D011994:Recombinant Proteins; D006639:Histidine; D009532:Nickel; D004247:DNA; D009571:Nitrilotriacetic Acid