title: Intracerebroventricular or intrathecal injection of glycine produces analgesia in thermal nociception and chemical nociception via glycine receptors.
abstract: The present study was designed to investigate the role of glycine receptors in analgesia induced by injection of glycine in vivo. Glycine was injected intracerebroventricularly or intrathecally and strychnine, a glycine receptor antagonist, was injected intracerebroventricularly or intrathecally before glycine injection. The effects on the pain threshold index in hot-plate test and the writhing times in acetic acid-induced writhing test were observed. The locomotor activity and motor performance (rotarod test) were also observed. The dosages of glycine and strychnine we choose had no effect on locomotor activity or motor performance in conscious mice. Glycine increased the pain threshold index in hot-plate test and decreased the writhing times of the mice. Strychnine antagonized the effects induced by glycine above. These results demonstrated that intracerebroventricular or intrathecal injection of glycine can produce analgesia in thermal nociception and chemical nociception in vivo, which is mediated by glycine receptors.
authors.affiliation: Jiangsu Province Key Laboratory of Anesthesiology, Xuzhou Medical College, Affiliated Hospital of Xuzhou Medical College, Jiangsu Province, Xuzhou, Jiangsu, PR China.
authors.forename: Wei
authors.initials: W
authors.lastname: Cheng
authors.forename: Qin
authors.initials: Q
authors.lastname: Yin
authors.forename: Ming-Yue
authors.initials: MY
authors.lastname: Cheng
authors.forename: Hong-Sheng
authors.initials: HS
authors.lastname: Chen
authors.forename: Shu
authors.initials: S
authors.lastname: Wang
authors.forename: Tao
authors.initials: T
authors.lastname: Feng
authors.forename: Yin-Ming
authors.initials: YM
authors.lastname: Zeng
authors.forename: Gong-Jian
authors.initials: GJ
authors.lastname: Liu
mesh_terms: D019342:Acetic Acid / Q000009:adverse effects; D000698:Analgesia; D000700:Analgesics / Q000008:administration & dosage* / Q000494:pharmacology*; D000818:Animals; D001522:Behavior, Animal / Q000187:drug effects; D005260:Female; D005998:Glycine / Q000008:administration & dosage* / Q000494:pharmacology*; D006358:Hot Temperature / Q000009:adverse effects*; D007276:Injections, Intraventricular; D007278:Injections, Spinal; D008297:Male; D051379:Mice; D009043:Motor Activity / Q000187:drug effects; D010146:Pain / Q000139:chemically induced / Q000188:drug therapy* / Q000378:metabolism / Q000503:physiopathology; D017288:Pain Threshold / Q000187:drug effects; D018009:Receptors, Glycine / Q000037:antagonists & inhibitors / Q000378:metabolism*; D045442:Rotarod Performance Test; D013331:Strychnine / Q000008:administration & dosage / Q000494:pharmacology
chemical_list: D000700:Analgesics; D018009:Receptors, Glycine; D013331:Strychnine; D019342:Acetic Acid; D005998:Glycine