Development of a high-sensitivity chromatographic separation system for pyridylaminated aldopentoses and aldohexoses.
A rare sugar is considered to be a monosaccharide rarely found in nature. To investigate their natural distribution and biological roles, a robust analytical system must be used to isolate, identify, and quantify them. Herein, we report the development of such a system that can specifically quantify and chromatographically separate four aldopentoses and eight aldohexoses tagged with 2-aminopyridine. Purified monosaccharides derivatized with a pyridylamino moiety (PA-monosaccharides) are first chromatographed over a high-performance anion-exchange resin. But, because two of the PA-aldohexoses used in this study, PA-talose and PA-idose, co-elute with the common saccharides, PA-glucose and PA-mannose, respectively, a second chromatographic step, reversed-phase high-performance liquid chromatography, is used to completely separate them. Thus, as shown by the results of this study, chromatographic separation of PA-monosaccharides is achievable and provides a quantitative measurement of common and rare isomeric aldopentoses and aldohexoses.
Nakakita
Shin-ichi
S
Department of Functional Glycomics, Life Science Research Center, Kagawa University, 1750-1 Ikenobe, Miki-cho, Kita-gun, Kagawa 761-0793, Japan.
Hasehira
Kayo
K
Hosokawa
Tomohiro
T
Tokuda
Masaaki
M
Izumori
Ken
K
Takegawa
Kaoru
K
Hirabayashi
Jun
J
D000631:Aminopyridines / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D009005:Monosaccharides / Q000737:chemistry / Q000302:isolation & purification*; D012680:Sensitivity and Specificity
D000631:Aminopyridines; D009005:Monosaccharides