Nucleotide sequence variation in the hexon gene of human adenovirus type 8 and 37 strains from epidemic keratoconjunctivitis patients in Japan.
Human adenovirus type 8 (HAdV-8) and 37 (HAdV-37) cause epidemic keratoconjunctivitis (EKC) associated with community-acquired and nosocomial infections. The nucleotide sequences of the entire hexon and fiber genes of eight HAdV-8 and 26 HAdV-37 strains were analysed and the transition mutations in each gene were compared among strains. Compared with prototype strains, the hexon gene of HAdV-8 and -37 strains showed between two and seven and one and twelve variations at nine and 21 different positions, respectively. All of these, except one position in HAdV-37, were located in the conserved region 4 (C4). There were only three polymorphisms in the fiber gene of both HAdV-8 and HAdV-37, fewer than those in C4. The nucleotide sequence of HAdV-8 and -37 C4 might be readily modified during EKC epidemics.
Kaneko
Hisatoshi
H
Department of Microbiology, Fukushima Medical University School of Medicine, Fukushima, Japan. h-kane@chive.ocn.ne.jp
Ishiko
Hiroaki
H
Ohguchi
Takeshi
T
Tagawa
Yoshitsugu
Y
Aoki
Koki
K
Suzutani
Tatsuo
T
Ohno
Shigeaki
S
D000258:Adenovirus Infections, Human / Q000453:epidemiology / Q000821:virology*; D000260:Adenoviruses, Human / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D001483:Base Sequence; D036022:Capsid Proteins / Q000235:genetics*; D004196:Disease Outbreaks; D014644:Genetic Variation; D006801:Humans; D007564:Japan / Q000453:epidemiology; D007637:Keratoconjunctivitis / Q000453:epidemiology / Q000821:virology*; D008969:Molecular Sequence Data; D009154:Mutation; D010802:Phylogeny
D036022:Capsid Proteins; C018315:hexon capsid protein, Adenovirus