title: One-pot fermentation of pladienolide D by Streptomyces platensis expressing a heterologous cytochrome P450 gene.
abstract: Pladienolide D is a 16-hydroxylated derivative of pladienolide B, produced by Streptomyces platensis. To facilitate the production of pladienolide D, the gene encoding a pladienolide B 16-hydroxylase from S. bungoensis was introduced into S. platensis. The recombinant produced pladienolide D at a production level comparable to that of pladienolide B.
authors.affiliation: Bioresource Laboratories, Mercian Corp., 1808 Nakaizumi, Iwata, Shizuoka 438-0078, Japan. machida-k@mercian.co.jp
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Machida
authors.forename: Yasuhide
authors.initials: Y
authors.lastname: Aritoku
authors.forename: Toshio
authors.initials: T
authors.lastname: Tsuchida
mesh_terms: D001426:Bacterial Proteins / Q000378:metabolism; D003001:Cloning, Molecular; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism*; D004852:Epoxy Compounds / Q000378:metabolism*; D005285:Fermentation; D018942:Macrolides / Q000378:metabolism*; D013302:Streptomyces / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D004852:Epoxy Compounds; D018942:Macrolides; C522342:pladienolide B; C522343:pladienolide D; D003577:Cytochrome P-450 Enzyme System