title: Sustained hypoxia promotes the development of a pulmonary artery-specific chronic inflammatory microenvironment.
abstract: Recent studies demonstrate that sustained hypoxia induces the robust accumulation of leukocytes and mesenchymal progenitor cells in pulmonary arteries (PAs). Since the factors orchestrating hypoxia-induced vascular inflammation are not well-defined, the goal of this study was to identify mediators potentially responsible for recruitment to and retention and differentiation of circulating cells within the hypoxic PA. We analyzed mRNA expression of 44 different chemokine/chemokine receptor, cytokine, adhesion, and growth and differentiation genes in PAs obtained via laser capture microdissection in adjacent lung parenchyma and in systemic arteries by RT-PCR at several time points of hypoxic exposure (1, 7, and 28 days) in Wistar-Kyoto rats. Analysis of inflammatory cell accumulation and protein expression of selected genes was concomitantly assessed by immunochemistry. We found that hypoxia induced progressive accumulation of monocytes and dendritic cells in the vessel wall with few T cells and no B cells or neutrophils. Upregulation of stromal cell-derived factor-1 (SDF-1), VEGF, growth-related oncogene protein-alpha (GRO-alpha), C5, ICAM-1, osteopontin (OPN), and transforming growth factor-beta (TGF-beta) preceded mononuclear cell influx. With time, a more complex pattern of gene expression developed with persistent upregulation of adhesion molecules (ICAM-1, VCAM-1, and OPN) and monocyte/fibrocyte growth and differentiation factors (TGF-beta, endothelin-1, and 5-lipoxygenase). On return to normoxia, expression of many genes (including SDF-1, monocyte chemoattractant protein-1, C5, ICAM-1, and TGF-beta) rapidly returned to control levels, changes that preceded the disappearance of monocytes and reversal of vascular remodeling. In conclusion, sustained hypoxia leads to the development of a complex, PA-specific, proinflammatory microenvironment capable of promoting recruitment, retention, and differentiation of circulating monocytic cell populations that contribute to vascular remodeling.
authors.affiliation: Department of Pediatrics and Medicine, University of Colorado Denver, USA.
authors.forename: Danielle L
authors.initials: DL
authors.lastname: Burke
authors.forename: Maria G
authors.initials: MG
authors.lastname: Frid
authors.forename: Claudia L
authors.initials: CL
authors.lastname: Kunrath
authors.forename: Vijaya
authors.initials: V
authors.lastname: Karoor
authors.forename: Adil
authors.initials: A
authors.lastname: Anwar
authors.forename: Brandie D
authors.initials: BD
authors.lastname: Wagner
authors.forename: Derek
authors.initials: D
authors.lastname: Strassheim
authors.forename: Kurt R
authors.initials: KR
authors.lastname: Stenmark
mesh_terms: D000818:Animals; D001013:Aorta, Thoracic / Q000502:physiology; D015815:Cell Adhesion Molecules / Q000235:genetics; D018925:Chemokines / Q000235:genetics; D002908:Chronic Disease; D016207:Cytokines / Q000235:genetics; D003713:Dendritic Cells / Q000276:immunology / Q000473:pathology; D005786:Gene Expression Regulation / Q000276:immunology; D006976:Hypertension, Pulmonary / Q000276:immunology*; D000860:Hypoxia / Q000276:immunology*; D036341:Intercellular Signaling Peptides and Proteins / Q000235:genetics; D008168:Lung / Q000098:blood supply / Q000502:physiology; D009000:Monocytes / Q000276:immunology / Q000473:pathology; D020411:Oligonucleotide Array Sequence Analysis; D011014:Pneumonia / Q000276:immunology* / Q000473:pathology; D011651:Pulmonary Artery / Q000276:immunology* / Q000473:pathology; D011652:Pulmonary Circulation / Q000276:immunology*; D051381:Rats; D011921:Rats, Inbred WKY; D019707:Receptors, Chemokine / Q000235:genetics
chemical_list: D015815:Cell Adhesion Molecules; D018925:Chemokines; D016207:Cytokines; D036341:Intercellular Signaling Peptides and Proteins; D019707:Receptors, Chemokine