title: Expression of epidermal growth factor receptor and vascular endothelial growth factor in malignant canine epithelial nasal tumours.
abstract: Epidermal growth factor (EGF) and vascular endothelial growth factor (VEGF) signalling pathways play a role in carcinogenesis. Inhibition of EGF receptor (EGFR) and of VEGF is effective in increasing the radiation responsiveness of neoplastic cells both in vitro and in human trials. In this study, immunohistochemical evaluation was employed to determine and characterize the potential protein expression levels and patterns of EGFR and VEGF in a variety of canine malignant epithelial nasal tumours. Of 24 malignant canine nasal tumours, 13 (54.2%) were positive for EGFR staining and 22 (91.7%) were positive for VEGF staining. The intensity and percentage of immunohistochemically positive neoplastic cells for EGFR varied. These findings indicate that EGFR and VEGF proteins were present in some malignant epithelial nasal tumours in the dogs, and therefore, it may be beneficial to treat canine patients with tumours that overexpress EGFR and VEGF with specific inhibitors in conjunction with radiation.
authors.affiliation: Department of Molecular Biomedical Science, College of Veterinary Medicine, North Carolina State University, Raleigh, NC, USA. kshiomitsu@vetmed.lsu.edu
authors.forename: K
authors.initials: K
authors.lastname: Shiomitsu
authors.forename: C L
authors.initials: CL
authors.lastname: Johnson
authors.forename: D E
authors.initials: DE
authors.lastname: Malarkey
authors.forename: A F
authors.initials: AF
authors.lastname: Pruitt
authors.forename: D E
authors.initials: DE
authors.lastname: Thrall
mesh_terms: D000818:Animals; D004283:Dog Diseases / Q000378:metabolism / Q000473:pathology* / Q000532:radiotherapy; D004285:Dogs; D066246:ErbB Receptors / Q000378:metabolism*; D015972:Gene Expression Regulation, Neoplastic; D007150:Immunohistochemistry / Q000662:veterinary; D009375:Neoplasms, Glandular and Epithelial / Q000378:metabolism / Q000473:pathology / Q000532:radiotherapy / Q000662:veterinary*; D009669:Nose Neoplasms / Q000532:radiotherapy / Q000662:veterinary*; D016896:Treatment Outcome; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism*
chemical_list: D042461:Vascular Endothelial Growth Factor A; D066246:ErbB Receptors