[Study on genetic relationship of Astragalus membranaceus var mongholicus in different producing area using SRAP].
OBJECTIVE
To study the genetic relationship of Astragalus membranaceus var. mongholicus in different producing area and provide theoretical basis for the evaluation of Astragalus germplasm resources.


METHODS
Through quence-related amplified polymorphism (SRAP) analysis, the systematic diagram of genetic relationship was constructed by UPGMA method.


RESULTS
A total of 141 SRAP markers were scored. By the use of UPGMA cluster analysis with genetic distance, Astragalus could be divided into two provenance plots of Gansu and Shanxi.


CONCLUSIONS
The genetic differentiation among populations of A. membranaceus var. mongholicus is remarkable. SRAP marker could be efficiently used for the study of the genetic relationship of Astragalus.
Qian
Dan
D
Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China.
Huang
Luqi
L
Cui
Guanghong
G
Chen
Min
M
D027883:Astragalus Plant / Q000145:classification / Q000235:genetics; D027885:Astragalus propinquus / Q000235:genetics*; D018744:DNA, Plant / Q000032:analysis*; D004365:Drugs, Chinese Herbal / Q000145:classification; D005819:Genetic Markers / Q000235:genetics*; D010802:Phylogeny; D011110:Polymorphism, Genetic; D012337:RNA, Ribosomal, 18S / Q000235:genetics
D018744:DNA, Plant; D004365:Drugs, Chinese Herbal; D005819:Genetic Markers; D012337:RNA, Ribosomal, 18S