Zeatin prevents amyloid beta-induced neurotoxicity and scopolamine-induced cognitive deficits.
The antioxidative and protective effects of zeatin against amyloid beta-protein (Abeta)-induced neurotoxicity were investigated using PC12 cells. Zeatin showed antioxidative and cell protective effects against Abeta-induced neurotoxicity. In this study, we also evaluated the effect of zeatin on learning and memory capacity in vivo using ICR mice with amnesia induced by scopolamine (1 mg/kg of body weight). Zeatin, when administered to mice at 4.5 mg/kg of body weight, significantly ameliorated scopolamine-induced amnesia as measured in both the passive avoidance test and Y-maze test. Injecting mice with scopolamine impaired performance on the passive avoidance test (48 +/- 4.5% decrease) and on the Y-maze test (12 +/- 1.3% decrease). In contrast, mice treated with zeatin before scopolamine injections were protected from these changes (5-34% decrease in step-through latency; 1-4% decrease in alternation behavior). The present results suggest a possible chemopreventive role of zeatin in Alzheimer's disease.
Choi
Soo Jung
SJ
Graduate School of Biotechnology, Korea University, Seoul, Republic of Korea.
Jeong
Chang-Ho
CH
Choi
Sung-Gil
SG
Chun
Ji-Yeon
JY
Kim
Young Jun
YJ
Lee
JeongMin
J
Shin
Dong-Hoon
DH
Heo
Ho Jin
HJ
D000544:Alzheimer Disease / Q000517:prevention & control; D000647:Amnesia / Q000139:chemically induced / Q000188:drug therapy*; D016229:Amyloid beta-Peptides / Q000009:adverse effects*; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology / Q000627:therapeutic use*; D001522:Behavior, Animal / Q000187:drug effects; D001921:Brain / Q000187:drug effects; D018680:Cholinergic Antagonists / Q000009:adverse effects; D003072:Cognition Disorders / Q000517:prevention & control*; D007858:Learning / Q000187:drug effects; D015227:Lipid Peroxidation / Q000187:drug effects; D008568:Memory / Q000187:drug effects; D051379:Mice; D008813:Mice, Inbred ICR; D031625:Morus / Q000737:chemistry; D020258:Neurotoxicity Syndromes / Q000188:drug therapy*; D016716:PC12 Cells; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology / Q000627:therapeutic use*; D018515:Plant Leaves; D020011:Protective Agents / Q000494:pharmacology / Q000627:therapeutic use; D051381:Rats; D017382:Reactive Oxygen Species / Q000037:antagonists & inhibitors; D012601:Scopolamine / Q000009:adverse effects; D015026:Zeatin / Q000494:pharmacology / Q000627:therapeutic use*
D016229:Amyloid beta-Peptides; D000975:Antioxidants; D018680:Cholinergic Antagonists; D010936:Plant Extracts; D020011:Protective Agents; D017382:Reactive Oxygen Species; D015026:Zeatin; D012601:Scopolamine