title: Molecular analysis of rice plants harboring a multi-functional T-DNA tagging system.
abstract: About 25,000 rice T-DNA insertional mutant lines were generated using the vector pCAS04 which has both promoter-trapping and activation-tagging function. Southern blot analysis revealed that about 40% of these mutants were single copy integration and the average T-DNA insertion number was 2.28. By extensive phenotyping in the field, quite a number of agronomically important mutants were obtained. Histochemical GUS assay with 4,310 primary mutants revealed that the GUS-staining frequency was higher than that of the previous reports in various tissues and especially high in flowers. The T-DNA flanking sequences of some mutants were isolated and the T-DNA insertion sites were mapped to the rice genome. The flanking sequence analysis demonstrated the different integration pattern of the right border and left border into rice genome. Compared with Arabidopsis and poplar, it is much varied in the T-DNA border junctions in rice.
authors.affiliation: State Key Laboratory of Plant Genomics and National Plant Gene Research Center (Beijing), Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.
authors.forename: Yimian
authors.initials: Y
authors.lastname: Ma
authors.forename: Luo
authors.initials: L
authors.lastname: Liu
authors.forename: Chengguang
authors.initials: C
authors.lastname: Zhu
authors.forename: Changhui
authors.initials: C
authors.lastname: Sun
authors.forename: Bo
authors.initials: B
authors.lastname: Xu
authors.forename: Jun
authors.initials: J
authors.lastname: Fang
authors.forename: Jiuyou
authors.initials: J
authors.lastname: Tang
authors.forename: Anding
authors.initials: A
authors.lastname: Luo
authors.forename: Shouyun
authors.initials: S
authors.lastname: Cao
authors.forename: Gupo
authors.initials: G
authors.lastname: Li
authors.forename: Qian
authors.initials: Q
authors.lastname: Qian
authors.forename: Yongbiao
authors.initials: Y
authors.lastname: Xue
authors.forename: Chengcai
authors.initials: C
authors.lastname: Chu
mesh_terms: D032461:Chromosomes, Plant / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics*; D017930:Genes, Reporter; D005822:Genetic Vectors / Q000235:genetics; D018745:Genome, Plant; D005966:Glucuronidase / Q000235:genetics / Q000378:metabolism; D016254:Mutagenesis, Insertional; D012275:Oryza / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D012231:Rhizobium / Q000235:genetics / Q000378:metabolism; D014170:Transformation, Genetic
chemical_list: D004269:DNA, Bacterial; C036483:T-DNA; D005966:Glucuronidase