title: High levels of E4-PHA-reactive oligosaccharides: potential as marker for cells with characteristics of hepatic progenitor cells.
abstract: Oligosaccharides serve as markers of the cell surface and have been used as certain kinds of tumor markers. In the present study, we established a simple method for isolating hepatic progenitor cells using a lectin, which recognizes a characteristic oligosaccharide structure. Rat liver epithelial (RLE) cells, which have been established as a hepatic stem-like cell, were used to identify characteristic oligosaccharide structures on hepatic stem cells. As a result from lectin micro array, several types of lectin including E4-PHA were identified to bind RLE cells specifically. Furthermore, lectin blot and lectin flow cytometry analyses showed that binding to E(4)-PHA lectin was significantly increased in RLE cells, compared to hepatocytes, and hepatoma cells. The induction of differentiation into a hepatocyte lineage of RLE cells by treatment with Oncostatin M and dexamethasone resulted in a decrease in E(4)-PHA binding. Using an E(4)-PHA column, we succeeded in isolating hepatic stem cells from LEC (Long-Evans with cinnamon coat color) rat livers with fluminant hepatitis. The characteristics of the established cells were similar to RLE cells and had a potential of proliferating in rat liver. These results suggest that oligosaccharides can serve as a novel marker for the isolation of the hepatic progenitor cells.
authors.affiliation: Department of Molecular Biochemistry & Clinical Investigation, Osaka University Graduate School of Medicine, 1-7 Yamada-oka, Suita, 565-0871, Japan.
authors.forename: Nozomi
authors.initials: N
authors.lastname: Sasaki
authors.forename: Kenta
authors.initials: K
authors.lastname: Moriwaki
authors.forename: Naofumi
authors.initials: N
authors.lastname: Uozumi
authors.forename: Katsuhisa
authors.initials: K
authors.lastname: Noda
authors.forename: Naoyuki
authors.initials: N
authors.lastname: Taniguchi
authors.forename: Akihiko
authors.initials: A
authors.lastname: Kameyama
authors.forename: Hisashi
authors.initials: H
authors.lastname: Narimatsu
authors.forename: Shunsaku
authors.initials: S
authors.lastname: Takeishi
authors.forename: Masao
authors.initials: M
authors.lastname: Yamada
authors.forename: Nobuto
authors.initials: N
authors.lastname: Koyama
authors.forename: Eiji
authors.initials: E
authors.lastname: Miyoshi
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D002240:Carbohydrate Sequence; D002454:Cell Differentiation; D049109:Cell Proliferation; D002469:Cell Separation; D004847:Epithelial Cells / Q000166:cytology / Q000378:metabolism; D037102:Lectins / Q000378:metabolism; D008099:Liver / Q000166:cytology*; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000737:chemistry / Q000378:metabolism*; D010835:Phytohemagglutinins / Q000378:metabolism*; D040081:Protein Array Analysis; D051381:Rats; D013234:Stem Cells / Q000166:cytology* / Q000378:metabolism*
chemical_list: D015415:Biomarkers; D037102:Lectins; D009844:Oligosaccharides; D010835:Phytohemagglutinins