title: Mitochondrial carrier family inventory of Trypanosoma brucei brucei: Identification, expression and subcellular localisation.
abstract: The mitochondrial carrier family (MCF) is a group of structurally conserved proteins that mediate the transport of a wide range of metabolic intermediates across the mitochondrial inner membrane. In this paper, an overview of the mitochondrial carrier proteins (MCPs) of the early-branching kinetoplastid parasite Trypanosoma brucei brucei is presented. Sequence analysis and phylogenetic reconstruction gave insight into the evolution and conservation of the 24 identified TbMCPs; for most of these, putative transport functions could be predicted. Comparison of the kinetoplastid MCP inventory to those previously reported for other eukaryotes revealed remarkable deviations: T. b. brucei lacks genes encoding some prototypical MCF members, such as the citrate carrier and uncoupling proteins. The in vivo expression of the identified TbMCPs in the two replicating life-cycle forms of T. b. brucei, the bloodstream-form and procyclic-form, was quantitatively assessed at the mRNA level by Northern blot analysis. Immunolocalisation studies confirmed that majority of the 24 identified TbMCPs is found in the mitochondrion of procyclic-form T. b. brucei.
authors.affiliation: Department of Biological Sciences and Hull York Medical School (HYMS), University of Hull, HU6 7RX Hull, United Kingdom. colasanteclaudia@gmx.de
authors.forename: Claudia
authors.initials: C
authors.lastname: Colasante
authors.forename: P
authors.initials: P
authors.lastname: Peña Diaz
authors.forename: Christine
authors.initials: C
authors.lastname: Clayton
authors.forename: Frank
authors.initials: F
authors.lastname: Voncken
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D015152:Blotting, Northern; D002352:Carrier Proteins / Q000032:analysis* / Q000235:genetics*; D016000:Cluster Analysis; D017124:Conserved Sequence; D020869:Gene Expression Profiling; D026901:Membrane Transport Proteins / Q000032:analysis / Q000235:genetics; D008856:Microscopy, Fluorescence; D024101:Mitochondrial Proteins / Q000032:analysis* / Q000235:genetics*; D008954:Models, Biological; D008958:Models, Molecular; D008969:Molecular Sequence Data; D017434:Protein Structure, Tertiary; D015800:Protozoan Proteins / Q000032:analysis* / Q000235:genetics*; D012333:RNA, Messenger / Q000096:biosynthesis; D017386:Sequence Homology, Amino Acid; D014346:Trypanosoma brucei brucei / Q000737:chemistry* / Q000235:genetics*
chemical_list: D002352:Carrier Proteins; D026901:Membrane Transport Proteins; D024101:Mitochondrial Proteins; D015800:Protozoan Proteins; D012333:RNA, Messenger