Low dietary inorganic phosphate affects the lung growth of developing mice.
Inorganic phosphate (Pi) plays a critical role in diverse cellular functions, and regulating the Pi balance is accomplished by sodium-dependent Pi co-transporter (NPT). Pulmonary NPT has recently been identified in mammalian lungs. However, to date, many of the studies that have involved Pi have mainly focused on its effect on bone and kidney. Therefore, current study was performed to discover the potential effects of low Pi on the lung of developing transgenic mice expressing the renilla/firefly luciferase dual reporter gene. Two-weeks old male mice divided into 2 groups and these groups were fed either a low PI diet or a normal control diet (normal: 0.5% Pi, low: 0.1% Pi) for 4 weeks. After 4 weeks of the diet, all the mice were sacrificed. Their lungs were harvested and analyzed by performing luciferase assay, Western blotting, kinase assay and immunohistochemistry. Our results demonstrate that low Pi affects the lungs of developing mice by disturbing protein translation, the cell cycle and the expression of fibroblast growth factor-2. These results suggest that optimally regulating Pi consumption may be important to maintain health.
Xu
Cheng Xiong
CX
Laboratory of Toxicology, College of Veterinary Medicine, Seoul National University, Seoul, Korea.
Jin
Hua
H
Chung
Youn Sun
YS
Shin
Ji Young
JY
Hwang
Soon Kyung
SK
Kwon
Jung Taek
JT
Park
Sung Jin
SJ
Lee
Eun Sun
ES
Minai-Tehrani
Arash
A
Chang
Seung Hee
SH
Woo
Min Ah
MA
Noh
Mi Suk
MS
An
Gil Hwan
GH
Lee
Kee Ho
KH
Cho
Myung Haing
MH
D048868:Adaptor Proteins, Signal Transducing; D000818:Animals; D015153:Blotting, Western; D002352:Carrier Proteins / Q000378:metabolism; D018797:Cell Cycle Proteins; D039642:Eukaryotic Initiation Factors; D007150:Immunohistochemistry; D008168:Lung / Q000187:drug effects / Q000201:enzymology / Q000254:growth & development* / Q000378:metabolism; D008297:Male; D051379:Mice; D008822:Mice, Transgenic; D010750:Phosphoproteins / Q000378:metabolism; D016226:Phosphorus, Dietary / Q000008:administration & dosage*; D010766:Phosphorylation; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000378:metabolism; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D050613:Sodium-Phosphate Cotransporter Proteins, Type IIa / Q000378:metabolism*; D058570:TOR Serine-Threonine Kinases
D048868:Adaptor Proteins, Signal Transducing; D002352:Carrier Proteins; D018797:Cell Cycle Proteins; C499527:Eif4ebp1 protein, mouse; D039642:Eukaryotic Initiation Factors; D010750:Phosphoproteins; D016226:Phosphorus, Dietary; D050613:Sodium-Phosphate Cotransporter Proteins, Type IIa; D017853:Phosphotransferases (Alcohol Group Acceptor); C546843:mTOR protein, mouse; D051057:Proto-Oncogene Proteins c-akt; D058570:TOR Serine-Threonine Kinases