[Gas chromatography-mass spectrometric analysis of Jatropha curcas leaf extracts prepared by supercritical fluid CO2 extraction].
OBJECTIVE
To analyze the bioactive components in Jatropha curcas leaves using gas chromatography-mass spectrometry (GC-MS).


METHODS
The bioactive components were extracted from J. curcas leaves by supercritical fluid CO2 extraction and analyzed by using GC-MS.


RESULTS
Seventy peaks were detected by GC-MS, and 43 compounds were identified (61.43%). Among the identified compounds, 16 had a content of more than 1%, and the total contents of these 16 compounds reached 81.36%. The four most abundant components were 22,23-dihydro-stigmasterol (16.14%), alpha-tocopherol (15.18%), beta-amylin (7.73%) and dotriacontanol (7.02%). The content of gamma-tocopherol reached 2.88% and vitamin E reached 18.06% in the extract.


CONCLUSIONS
J. curcas leaves contain multiple compounds with anti-tumor, anti-virus and antimicrobial activities.
Wang
Zhao-yu
ZY
School of Life Science and Biopharmacology, Guangdong Pharmaceutical University, and Department of Pharmaceutics, Zhujiang Hospital, Guangzhou 510006, China. wangzhaoyu77@yahoo.com.cn
Chen
Fei-long
FL
Lin
Jing-ming
JM
Huang
Shu-lin
SL
D025924:Chromatography, Supercritical Fluid / Q000379:methods; D008401:Gas Chromatography-Mass Spectrometry; D031286:Jatropha / Q000737:chemistry*; D010936:Plant Extracts / Q000032:analysis* / Q000302:isolation & purification; D018515:Plant Leaves / Q000737:chemistry
D010936:Plant Extracts