Cartilage replacement by use of hybrid systems of autologous cells and polyethylene: an experimental study.
This study used porous polyethylene (PE) as a scaffold in an animal model system. The surface of the scaffolds was either modified with collagen II coating or first functionalized by oxygen plasma treatment and then coated with collagen II. The specimens were inoculated with autologous chondrocytes and transplanted into the concha of guinea pigs. Bare scaffolds were used as controls. Periods of 1, 6, and 12 months after implantation, samples of cells containing specimens and control samples were evaluated microscopically. As a result, the pre-seeded specimens were better integrated into the surrounding tissue than cell-free PE-specimens. Also a weaker immune reaction and an improved cartilage generation could be detected in the pre-seeded specimen. Compared to the other surface modifications, no further improvement of cartilage development was observed in the long term in vivo animal experimental study.
Schoen
Ilona
I
Department of Otorhinolaryngology, Head and Neck Surgery, University Hospital MLU Halle-Wittenberg, 06112 Halle, Germany. ilona.schoen@medizin.uni-halle.de
Rahne
Torsten
T
Markwart
Annekatrin
A
Neumann
Kerstin
K
Berghaus
Alexander
A
Roepke
Ernst
E
D000818:Animals; D002356:Cartilage / Q000187:drug effects / Q000502:physiology; D002478:Cells, Cultured; D019902:Chondrocytes / Q000187:drug effects / Q000637:transplantation*; D003094:Collagen / Q000378:metabolism; D003131:Combined Modality Therapy; D005260:Female; D015743:Giant Cells, Foreign-Body / Q000502:physiology; D048091:Guided Tissue Regeneration / Q000379:methods*; D006168:Guinea Pigs; D023421:Models, Animal; D020959:Polyethylene / Q000737:chemistry / Q000494:pharmacology*; D011485:Protein Binding / Q000187:drug effects; D012038:Regeneration / Q000187:drug effects; D013997:Time Factors; D054457:Tissue Scaffolds / Q000737:chemistry*; D014182:Transplantation, Autologous
D020959:Polyethylene; D003094:Collagen