Analytical contribution to the determination of the purity and the degree of polymerization of Hyaluronic Acid.
Analytical contribution to the determination of the purity and the degree of polymerization of Hyaluronic Acid Hyaluronic Acid (H.A.) is of importance in cosmetic formulation because of its physical chemical properties and its role in biological tissues; it occurs in association with chondroitin sulphates and a method of analysis of separating the two substances has been developed. The method is based on gel permeation chromatography, or 'exclusion'chromatography, and requires extremely small quantities of product. The conditions which have been found give two neighbouring peaks of which the surfaces are proportional to the contents of H.A. and the chondroitin sulphates. In addition to the quantity, the shape of the H.A. peak and the relative retention volume give further information as to the polymerization character of the sample. Using a known control sample it is possible, in 30 minutes, to determine the relative proportions of the polysaccharides H.A. and chondroitin sulphates and to determine the degree of polymerization of the H.A. Results are given of the analysis of a commercial sample of H.A.
David
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Centre d'Analyses et de Recherches Expérimentales de Fougeres (CAREF), la Boitardière, Javene, 35133 Fougeres, France.
Blandel
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