CD26/DPPIV cell membrane expression and DPPIV activity in plasma of patients with acute leukemia.
CD26/DPPIV (dipeptidil peptidase IV) displays an array of diverse functional properties, with a role in the development of several human cancers. This enzyme is found mainly anchored in the membrane of cells although it also has an enzymatically active plasma isoform. The regulation of biological activities of cytokines by DPP IV activity has a potential role in the homeostatic regulation of hematopoiesis. In this study, we analyzed the CD26 antigen cell membrane expression by flow cytometry and the DPPIV activity in plasma of patients of acute leukemia. The results showed that the plasma DPPIV activity is significantly higher in leukemia patients and could be 100% inhibited by Januvia (Merck Sharp & Dohme) a selective DPPIV inhibitor. Although CD26 expression on immune cells were not leukemia-dependent the analysis of the correlation between CD26 expression and the DPPIV plasma activity were statistically significant (p < 0.01) in acute lymphoid leukemia (B-ALL and T-ALL).
de Andrade
Camilla F C G
CF
Laboratório de Genética Aplicada, Serviço de Hematologia do Hospital do Câncer I, Instituto Nacional de Câncer, Praça da Cruz Vermelha 23CEP 20230-130, Rio de Janeiro, RJ, Brazil.
Bigni
Ricardo
R
Pombo-de-Oliveira
Maria S
MS
Alves
Gilda
G
Pereira
Denise A
DA
D000293:Adolescent; D002462:Cell Membrane / Q000737:chemistry / Q000378:metabolism*; D002648:Child; D018819:Dipeptidyl Peptidase 4 / Q000097:blood* / Q000378:metabolism*; D054873:Dipeptidyl-Peptidase IV Inhibitors; D004305:Dose-Response Relationship, Drug; D005434:Flow Cytometry; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D016130:Immunophenotyping; D007700:Kinetics; D008213:Lymphocyte Activation / Q000187:drug effects / Q000235:genetics / Q000502:physiology; D054218:Precursor T-Cell Lymphoblastic Leukemia-Lymphoma / Q000097:blood* / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D011719:Pyrazines / Q000097:blood / Q000494:pharmacology; D012016:Reference Values; D000068900:Sitagliptin Phosphate; D014230:Triazoles / Q000097:blood / Q000494:pharmacology; D055815:Young Adult
D054873:Dipeptidyl-Peptidase IV Inhibitors; D011719:Pyrazines; D014230:Triazoles; C042807:DPP4 protein, human; D018819:Dipeptidyl Peptidase 4; D000068900:Sitagliptin Phosphate