title: Atrial natriuretic peptide decreases aorta stiffness in cholesterol-fed anesthetized rabbits.
abstract: Aortas from Watanabe heritable hyperlipidemic rabbits show in vitro impaired vasodilatory response to atrial natriuretic peptide (ANP) during atherosclerosis progression. To test a similar reaction in vivo, the effect of ANP administration on pulse wave velocity (PWV, index of aorta stiffness) was investigated in 10 normal and 10 cholesterol-fed (2% cholesterol-loaded feeding for 4 weeks) anesthetized male New Zealand White (NZW) rabbits. Invasively taken carotid and femoral blood pressures (BP) were recorded, simultaneously with ECG, and blood samples for ANP measurement (by RIA) were taken at 0 min and 20, 40, 60 min following an intravenous 20-min administration of either 0.2 microg kg(-1)min(-1) hANP in 5 ml normal saline or only 5 ml saline. Mild to moderate atherosclerosis was found in ascending aorta. BP decreased by ANP only at 20 min in both groups, whereas only in cholesterol-fed rabbits the borderline (p=0.09) increased at 0 min PWV was lowered (p=0.008) in all recording times. With any degree of increase of systolic BP (SBP) PWV increased less in ANP receivers. Atherosclerosis and SBP were the most important determinants of PWV and the effect of ANP was independent of confounding factors. It is concluded that short-term ANP administration in doses to achieve levels approximately threefold the pretreatment ones in normal and mildly to moderately atherosclerotic anesthetized NZW rabbits, causes an improvement of aorta stiffness only in atherosclerotic rabbits.
authors.affiliation: Department of Experimental Physiology, Medical School, Aristotle University, University Campus, 54124 Thessaloniki, Greece. kallaras@med.auth.gr
authors.forename: Konstantinos
authors.initials: K
authors.lastname: Kallaras
authors.forename: George
authors.initials: G
authors.lastname: Babas
authors.forename: Vassiliki
authors.initials: V
authors.lastname: Stergiou-Michailidou
authors.forename: Michael
authors.initials: M
authors.lastname: Karamouzis
authors.forename: Thomas
authors.initials: T
authors.lastname: Zaraboukas
mesh_terms: D000818:Animals; D001011:Aorta / Q000473:pathology* / Q000503:physiopathology; D050197:Atherosclerosis / Q000473:pathology / Q000503:physiopathology*; D009320:Atrial Natriuretic Factor / Q000008:administration & dosage / Q000097:blood / Q000378:metabolism*; D001794:Blood Pressure; D002791:Cholesterol, Dietary / Q000008:administration & dosage / Q000378:metabolism*; D004562:Electrocardiography; D006339:Heart Rate; D008055:Lipids / Q000097:blood; D008297:Male; D011817:Rabbits
chemical_list: D002791:Cholesterol, Dietary; D008055:Lipids; D009320:Atrial Natriuretic Factor