title: [Study of expression of TLR9, NF-kappaB, TNFalpha genes in cells of cervical canal mucosa in pregnant women with herpesvirus infection].
abstract: OBJECTIVE
  To study changes in expression of TLR9, NF-kappaB, TNFalpha genes in cervical canal mucosa in pregnant women with herpesvirus infection.
  
  
  METHODS
  Using previously developed real-time polymerase chain reaction assays, levels of expression of genes, which corresponding proteins play important role in TLR9-dependent signaling pathway, was quantified.
  
  
  RESULTS
  Two-fold, 4.4-fold, and 3-fold rise in expression of TLR9, NF-kappaB, and TNFalpha genes respectively was revealed in pregnant women with herpesvirus infection compared to women with physiologically occurring pregnancy.
  
  
  CONCLUSIONS
  Obtained results demonstrated the activation of TLR-mediated mechanisms of innate immunity on the level of cervical canal mucosa during pregnancy complicated by viral infection.
authors.forename: O A
authors.initials: OA
authors.lastname: Gankovskaia
authors.forename: I V
authors.initials: IV
authors.lastname: Bakhareva
authors.forename: L V
authors.initials: LV
authors.lastname: Gankovskaia
authors.forename: O Iu
authors.initials: OIu
authors.lastname: Somova
authors.forename: V V
authors.initials: VV
authors.lastname: Zverev
mesh_terms: D000328:Adult; D002584:Cervix Uteri / Q000276:immunology* / Q000821:virology; D005260:Female; D015870:Gene Expression; D006566:Herpesviridae Infections / Q000235:genetics / Q000276:immunology*; D006801:Humans; D009092:Mucous Membrane / Q000276:immunology / Q000821:virology; D016328:NF-kappa B / Q000235:genetics*; D011247:Pregnancy; D011251:Pregnancy Complications, Infectious / Q000235:genetics / Q000276:immunology* / Q000821:virology; D051217:Toll-Like Receptor 9 / Q000235:genetics*; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics*; D015854:Up-Regulation
chemical_list: D016328:NF-kappa B; C495371:TLR9 protein, human; D051217:Toll-Like Receptor 9; D014409:Tumor Necrosis Factor-alpha