Isolation and structure elucidation of two different polysaccharides from the lipopolysaccharide of Rahnella aquatilis 33071T.
Two different polysaccharides were obtained by mild acid degradation of the lipopolysaccharide of Rahnella aquatilis 33071(T). These were studied by sugar and methylation analyses along with 1D and 2D (1)H and (13)C NMR spectroscopy. The following structures were established for the polysaccharides: -->4)-alpha-D-Rhap-(1-->3)-alpha-D-Rhap-(1-->3)beta-D-Galp-(1-->3)-alpha-D-Manp-(1-->2)-alpha-D-Manp-(1--> beta-D-Glcp-(1-->3)-alpha-D-galp-(1-->4)-alpha-D-GlcpA-(1-->2). The former structure is new, whereas the latter has been reported earlier as the structure of the O-specific polysaccharide of R. aquatilis 95 U003 (Zdorovenko, E. L.; Varbanets, L. D.; Zatonsky, G. V.; Kachala, V. V.; Zdorovenko, G. M.; Shashkov, A. S.; Knirel, Y. A. Carbohydr. Res.2008, 343, 2494-2497).
Zdorovenko
Evelina L
EL
N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Leninsky Prospeckt 47, 119991 Moscow, Russia. evelina@ioc.ac.ru
Varbanets
Ludmila D
LD
Zatonsky
George V
GV
Zdorovenko
Galina M
GM
Shashkov
Alexander S
AS
Knirel
Yuriy A
YA
D002240:Carbohydrate Sequence; D008070:Lipopolysaccharides / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D011134:Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification*; D020638:Rahnella / Q000737:chemistry*
D008070:Lipopolysaccharides; D011134:Polysaccharides