title: Organic solvent extraction and metabonomic profiling of the metabolites in erythrocytes.
abstract: We used erythrocytes as the model tissue to evaluate an optimal solution for the extraction of intracellular metabolites and time-dependent variation of the metabolome in living cells. Projection to latent structure (PLS) of the GC/MS and LC/MS data suggested that the most efficient solution for the extraction of metabolites from wet erythrocytes (50 mg) could be a methanol-chloroform-water mixture (950 microL, 700:200:50, v/v/v). PLS-discriminant analysis (DA) clearly profiled a time-dependent alternation of metabolic phenotype of erythrocytes. Identification of the metabolites showed that the process was characterized by accumulating of metabolic products and depleting of nutritious substances in erythrocytes during incubation.
authors.affiliation: Laboratory of Metabonomics, China Pharmaceutical University, Nanjing 210009, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhang
authors.forename: Jiye
authors.initials: J
authors.lastname: A
authors.forename: Guangji
authors.initials: G
authors.lastname: Wang
authors.forename: Qing
authors.initials: Q
authors.lastname: Huang
authors.forename: Bei
authors.initials: B
authors.lastname: Yan
authors.forename: Weibin
authors.initials: W
authors.lastname: Zha
authors.forename: Shenghua
authors.initials: S
authors.lastname: Gu
authors.forename: Linsheng
authors.initials: L
authors.lastname: Liu
authors.forename: Hongcan
authors.initials: H
authors.lastname: Ren
authors.forename: Meiting
authors.initials: M
authors.lastname: Ren
authors.forename: Longsheng
authors.initials: L
authors.lastname: Sheng
mesh_terms: D000328:Adult; D004912:Erythrocytes / Q000378:metabolism*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006801:Humans; D008297:Male; D055432:Metabolomics / Q000379:methods*; D000432:Methanol / Q000737:chemistry; D012997:Solvents / Q000737:chemistry*
chemical_list: D012997:Solvents; D000432:Methanol