title: [Clinical research of AFP variant with microcentrifugalcolumn method and crossed affinity immunoelectrophoresis autoradiography method].
abstract: OBJECTIVE
  To compare the clinical value of microcentrifugalcolumn method with crossed affinity immunoelectrophoresis autoradiography method for the measurement of alpha-fetoprotein variant (AFP-L3) in differentiation of benign and malignant liver.
  
  
  METHODS
  Serum AFP-L3 variants in 102 primary hepatocellular carcinoma patients and 41 chronic and cirrhosis patients were separated by microcentrifugalcolumn method and crossed affinity immunoelectrophoresis autoradiography method and the clinical value of both method were compared.
  
  
  RESULTS
  In 102 primary hepatocellular carcinoma patients, the sensitivity of AFP-L3 was 79.4% and 91.2% respectively, in 41 chronic and cirrhosis patients. The specificity of AFP-L3 was 70.7% and 29.3% respectively. The diagnostic accuracy was 76.9% and 73.4% respectively. The area in the ROC curve was 0.791 and 0.758 respectively. In the 4 primary hepatocellular carcinoma patients with lower AFP-L3, the AFP-L3 was positive by useing microcentrifugalcolumn method, but none of AFP-L3 were found by useing crossed affinity immunoelectrophoresis autoradiography method.
  
  
  CONCLUSIONS
  The micro centrifugalcolumn method is more simple and rapid, it may be more useful in differentiation of benign and malignant liver than traditional crossed affinity immunoelectrophoresis autoradiography method.
authors.affiliation: Fujian Oncology Hospital, Fuzhou 350014, China. yanc99@sina.com
authors.forename: Yan
authors.initials: Y
authors.lastname: Chen
authors.forename: Ying-ying
authors.initials: YY
authors.lastname: Lin
authors.forename: Min-hua
authors.initials: MH
authors.lastname: Hu
authors.forename: Yan-song
authors.initials: YS
authors.lastname: Chen
mesh_terms: D000328:Adult; D000368:Aged; D001345:Autoradiography / Q000379:methods*; D006528:Carcinoma, Hepatocellular / Q000737:chemistry; D005260:Female; D006801:Humans; D007122:Immunoelectrophoresis / Q000379:methods*; D008297:Male; D008875:Middle Aged; D020033:Protein Isoforms / Q000235:genetics* / Q000276:immunology; D000509:alpha-Fetoproteins / Q000235:genetics* / Q000276:immunology
chemical_list: D020033:Protein Isoforms; D000509:alpha-Fetoproteins