[Clinical research of AFP variant with microcentrifugalcolumn method and crossed affinity immunoelectrophoresis autoradiography method].
OBJECTIVE
To compare the clinical value of microcentrifugalcolumn method with crossed affinity immunoelectrophoresis autoradiography method for the measurement of alpha-fetoprotein variant (AFP-L3) in differentiation of benign and malignant liver.


METHODS
Serum AFP-L3 variants in 102 primary hepatocellular carcinoma patients and 41 chronic and cirrhosis patients were separated by microcentrifugalcolumn method and crossed affinity immunoelectrophoresis autoradiography method and the clinical value of both method were compared.


RESULTS
In 102 primary hepatocellular carcinoma patients, the sensitivity of AFP-L3 was 79.4% and 91.2% respectively, in 41 chronic and cirrhosis patients. The specificity of AFP-L3 was 70.7% and 29.3% respectively. The diagnostic accuracy was 76.9% and 73.4% respectively. The area in the ROC curve was 0.791 and 0.758 respectively. In the 4 primary hepatocellular carcinoma patients with lower AFP-L3, the AFP-L3 was positive by useing microcentrifugalcolumn method, but none of AFP-L3 were found by useing crossed affinity immunoelectrophoresis autoradiography method.


CONCLUSIONS
The micro centrifugalcolumn method is more simple and rapid, it may be more useful in differentiation of benign and malignant liver than traditional crossed affinity immunoelectrophoresis autoradiography method.
Chen
Yan
Y
Fujian Oncology Hospital, Fuzhou 350014, China. yanc99@sina.com
Lin
Ying-ying
YY
Hu
Min-hua
MH
Chen
Yan-song
YS
D000328:Adult; D000368:Aged; D001345:Autoradiography / Q000379:methods*; D006528:Carcinoma, Hepatocellular / Q000737:chemistry; D005260:Female; D006801:Humans; D007122:Immunoelectrophoresis / Q000379:methods*; D008297:Male; D008875:Middle Aged; D020033:Protein Isoforms / Q000235:genetics* / Q000276:immunology; D000509:alpha-Fetoproteins / Q000235:genetics* / Q000276:immunology
D020033:Protein Isoforms; D000509:alpha-Fetoproteins