Non-lethal PCR genotyping of single Drosophila.
In Drosophila, genetic techniques relying on stochastic chromosomal rearrangements involve the generation and screening of a large number of fly stocks to isolate a few lines of interest. Here, we describe a PCR-based method allowing non-lethal molecular characterization of single flies. Using this procedure, individual candidate recombinant animals can be genotyped and selected one generation earlier than with extant methodology and, importantly, before stocks are established. This advance should significantly facilitate several of the most fundamental and routine techniques in Drosophila genetics.
Carvalho
Gil B
GB
Division of Biology, California Institute of Technology, Pasadena, CA 91125, USA. gil@caltech.edu
Ja
William W
WW
Benzer
Seymour
S
D055011:Ablation Techniques; D000818:Animals; D004251:DNA Transposable Elements; D004330:Drosophila / Q000235:genetics*; D005260:Female; D005298:Fertility / Q000502:physiology; D005838:Genotype; D008297:Male; D016133:Polymerase Chain Reaction; D011995:Recombination, Genetic; D014921:Wings, Animal / Q000737:chemistry
D004251:DNA Transposable Elements