Engraftment of bone marrow stromal cells in lipopolysaccharide-injured mouse lungs.
OBJECTIVE
To explore a feasibility of engraftment of systemically transplanted bone marrow stromal cells (BMSCs) and differentiation into lung epithelial cells in lipopolysaccharides (LPS)-injured lungs.


METHODS
BMSCs were isolated from bone marrow of transgenic green fluorescent protein (GFP) C57BL/6J mice and systemically administered to bone marrow-suppressed wild-type C57BL/6J mice. A mouse model of lung injury was prepared by intratracheal instillation of LPS. Recipients were assigned to four groups: intratracheal PBS + BMSCs transplantation (CM), intratracheal LPS + BMSCs transplantation (LM), intratracheal PBS + irradiation + BMSCs transplantation (CIM) and intratracheal LPS+ irradiation + BMSCs transplantation (LIM). BMSCs engraftment in recipient lungs was determined by immunofluorescent staining 14 days after BMSCs administration. Alveolar epithelial type II cells were isolated from recipient lungs and the rate of GFP positive cells was measured by flow cytometry. Expression of surfactant protein (SP)-A, SP-C and aquaporin (AQP)-5 mRNA in the lungs was evaluated by real-time PCR.


RESULTS
GFP and cytokeratin positive cells were observed in lung parenchyma of the CIM and the LIM groups, but not in the CM and the LM groups. The LIM group had more positive cells than the CIM group. The rates of GFP positive cells were higher in the CIM (11.10+/- 3.19%) and the LIM groups (14.40+/- 2.40%) than those in the CM and the LM groups (2.82+/- 1.03% and 3.81+/- 0.93%, respectively; P< 0.05). The LIM group had higher mRNA expression of SP-C than the CM group (2.09+/- 0.18 vs 1.38+/- 0.30; P< 0.05).


CONCLUSIONS
Donor derived BMSCs can engraft in LPS-injured lungs and differentiate into lung epithelial cells, suggesting BMSCs transplantation might contribute to lung repair.
Wang
Wei
W
Laboratory of Pediatric Respiratory and Critical Care Medicine, Children's Hospital and the Institutes of Biomedical Sciences, Fudan University, Shanghai, China.
Qian
Li-Ling
LL
Liu
Hai-Pei
HP
Sun
Bo
B
D000818:Animals; D051402:Aquaporin 5 / Q000235:genetics; D001854:Bone Marrow Cells / Q000166:cytology*; D002454:Cell Differentiation; D005260:Female; D036341:Intercellular Signaling Peptides and Proteins; D008070:Lipopolysaccharides / Q000633:toxicity*; D008168:Lung / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D055370:Lung Injury / Q000628:therapy*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D010455:Peptides / Q000235:genetics; D037662:Pulmonary Surfactant-Associated Protein A / Q000235:genetics; D037721:Pulmonary Surfactant-Associated Protein C; D012333:RNA, Messenger / Q000032:analysis; D017154:Stromal Cells / Q000166:cytology / Q000637:transplantation*
C496038:Aqp5 protein, mouse; D051402:Aquaporin 5; D036341:Intercellular Signaling Peptides and Proteins; D008070:Lipopolysaccharides; D010455:Peptides; D037662:Pulmonary Surfactant-Associated Protein A; D037721:Pulmonary Surfactant-Associated Protein C; D012333:RNA, Messenger; C489680:Sftpc protein, mouse