[Oxidized low-density lipoprotein enhances the expressions of SREBP-2 and HMGCR mRNA in macrophages derived from the monocytes of patients with acute coronary syndrome].
OBJECTIVE
To investigate the effect of oxidized low-density lipoprotein (ox-LDL) on the expressions of sterol regulatory element binding protein-2 (SREBP-2) and hydroxymethylglutaryl CoA reductase (HMGCR) in the macrophages derived from monocytes of patients with acute coronary syndrome (ACS).


METHODS
LDL was oxidized by Cu2+ to prepare ox-LDL, and peripheral monocytes were isolated by density gradient centrifugation from patients with ACS diagnosed by coronary arteriography. Macrophages derived from the monocytes after phorbol myristate acetate (PMA) stimulation were treated with ox-LDL at the concentrations of 0, 20, 40, and 100 ng/ml, and the changes in the expressions of SREBP-2 and HMGCR were detected by real-time RT-PCR.


RESULTS
Compared with the control cells, the macrophages treated with ox-LDL showed significantly increased expressions of SREBP-2 and HMGCR mRNA (P<0.05). In cells treated with ox-LDL, the expressions of SREBP-2 and HMGCR mRNA differed significantly with the dose administered (P<0.05).


CONCLUSIONS
Within a defined dose range, ox-LDL can dose-dependently enhance the expressions of SREBP-2 and HMGCR mRNA in macrophages from patients with ACS.
Zhang
Pei-dong
PD
Department of Cardiovascular Medicine, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, China. Zhangpeidong120@sina.com
Liu
Ying-feng
YF
Guo
Yang
Y
Miao
Fei
F
Yu
Zhi-guo
ZG
Wang
Shi-xiang
SX
D054058:Acute Coronary Syndrome / Q000097:blood*; D004305:Dose-Response Relationship, Drug; D006801:Humans; D006903:Hydroxymethylglutaryl CoA Reductases / Q000235:genetics / Q000378:metabolism*; D008077:Lipoproteins, LDL / Q000494:pharmacology*; D008264:Macrophages / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051782:Sterol Regulatory Element Binding Protein 2 / Q000235:genetics / Q000378:metabolism*
D008077:Lipoproteins, LDL; D012333:RNA, Messenger; C497390:SREBF2 protein, human; D051782:Sterol Regulatory Element Binding Protein 2; C099252:oxidized low density lipoprotein; D006903:Hydroxymethylglutaryl CoA Reductases