Gamma-radiation-induced phosphorylation of p53 on serine 15 is dose-dependent in MOLT-4 leukaemia cells.
Molecular indicators of the absorbed dose of ionizing radiation are powerful tools in biodosimetry. The studies reported here were undertaken with the motivation to find such a marker among the mo lecules involved in ataxia-telangiectasia mutated kinase- dependent signalling induced by ionizing radiation (ATM-kinase, checkpoint kinase-2, protein p53, and oncoprotein Mdm2). In our previous work on T-lymphocyte leukaemia MOLT-4 cells we described the mentioned molecules of ATM-dependent pathway and none of them showed a pronounced dosedependent response. Here we employed Western blotting and ELISA assay to investigate the response of post-translationally modified p53 (particularly phosphorylated on serine 15) after gamma-irradiation. We have found the amount of phosphorylated p53 to be homogenously increased after irradiation by the doses of 0.5 to 7.5 Gy. The dose-dependent response was pronounced especially after the doses up to 3.0 Gy. The presented data indicate that p53 phosphorylated on serine 15 might be used as a potential biodosimetric marker.
Tichý
A
A
Department of Radiobiology, Faculty of Health Sciences, University of Defence in Brno, Hradec Králové, Czech Republic.
Záskodová
D
D
Zoelzer
F
F
Vávrová
J
J
Sinkorová
Z
Z
Pejchal
J
J
Osterreicher
J
J
Rezácová
M
M
D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000528:radiation effects; D005720:Gamma Rays; D006801:Humans; D007938:Leukemia; D010766:Phosphorylation / Q000528:radiation effects; D012694:Serine / Q000378:metabolism*; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism*
D016159:Tumor Suppressor Protein p53; D012694:Serine