title: Atomic-level mapping of antibody epitopes on a GPCR.
abstract: Epitopes that define the immunodominant regions of conformationally complex integral membrane proteins have been difficult to reliably delineate. Here, a high-throughput approach termed shotgun mutagenesis was used to map the binding epitopes of five different monoclonal antibodies targeting the GPCR CCR5. The amino acids, and in some cases the atoms, that comprise the critical contact points of each epitope were identified, defining the immunodominant structures of this GPCR and their physicochemistry.
authors.affiliation: Integral Molecular, Inc., 3701 Market Street, Fourth Floor, Philadelphia, Pennsylvania 19104, USA.
authors.forename: Cheryl
authors.initials: C
authors.lastname: Paes
authors.forename: Jada
authors.initials: J
authors.lastname: Ingalls
authors.forename: Karan
authors.initials: K
authors.lastname: Kampani
authors.forename: Chidananda
authors.initials: C
authors.lastname: Sulli
authors.forename: Esha
authors.initials: E
authors.lastname: Kakkar
authors.forename: Meredith
authors.initials: M
authors.lastname: Murray
authors.forename: Valery
authors.initials: V
authors.lastname: Kotelnikov
authors.forename: Tiffani A
authors.initials: TA
authors.lastname: Greene
authors.forename: Joseph B
authors.initials: JB
authors.lastname: Rucker
authors.forename: Benjamin J
authors.initials: BJ
authors.lastname: Doranz
mesh_terms: D000911:Antibodies, Monoclonal / Q000737:chemistry / Q000276:immunology*; D018604:Epitope Mapping / Q000379:methods*; D005455:Fluorescent Antibody Technique / Q000379:methods; D016056:Immunodominant Epitopes / Q000032:analysis*; D008958:Models, Molecular; D016296:Mutagenesis; D016133:Polymerase Chain Reaction / Q000379:methods; D019713:Receptors, CCR5 / Q000235:genetics / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D016056:Immunodominant Epitopes; D019713:Receptors, CCR5