Quantitative determination of urea concentrations in cell culture medium.
Urea is the major nitrogenous end product of protein metabolism in mammals. Here, we describe a quantitative, sensitive method for urea determination using a modified Jung reagent. This assay is specific for urea and is unaffected by ammonia, a common interferent in tissue and cell cultures. We demonstrate that this convenient colorimetric microplate-based, room temperature assay can be applied to determine urea synthesis in cell culture.
Zawada
Robert J X
RJ
BioAssay Systems LLC, Hayward, CA, USA.
Kwan
Peggy
P
Olszewski
Kellen L
KL
Llinas
Manuel
M
Huang
Shu-Gui
SG
D000818:Animals; D002138:Calibration; D003470:Culture Media / Q000737:chemistry*; D010963:Plasmodium falciparum / Q000166:cytology; D012015:Reference Standards; D014508:Urea / Q000032:analysis* / Q000592:standards
D003470:Culture Media; D014508:Urea