title: T-cadherin activates Rac1 and Cdc42 and changes endothelial permeability.
abstract: In the present study, expression of T-cadherin was shown to induce intracellular signaling in NIH3T3 fibroblasts: it activated Rac1 and Cdc42 (p < 0.01) but not RhoA. T-Cadherin overexpression in human umbilical vein endothelial cells (HUVEC) using adenoviral constructs induced disassembly of microtubules and polymerization of actin stress fibers, whereas down-regulation of endogenous T-cadherin expression in HUVEC using lentiviral constructs resulted in microtubule polymerization and a decrease in the number of actin stress fibers. Moreover, suppression of the T-cadherin expression significantly decreased the endothelial monolayer permeability as compared to the control (p < 0.001).
authors.affiliation: Institute of Experimental Cardiology, Cardiology Research Center, Moscow, 121552, Russia.
authors.forename: E V
authors.initials: EV
authors.lastname: Semina
authors.forename: K A
authors.initials: KA
authors.lastname: Rubina
authors.forename: P N
authors.initials: PN
authors.lastname: Rutkevich
authors.forename: T A
authors.initials: TA
authors.lastname: Voyno-Yasenetskaya
authors.forename: Y V
authors.initials: YV
authors.lastname: Parfyonova
authors.forename: V A
authors.initials: VA
authors.lastname: Tkachuk
mesh_terms: D000818:Animals; D015820:Cadherins / Q000235:genetics / Q000378:metabolism*; D002463:Cell Membrane Permeability; D002478:Cells, Cultured; D015536:Down-Regulation; D042783:Endothelial Cells / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D051379:Mice; D008870:Microtubules / Q000378:metabolism; D041681:NIH 3T3 Cells; D015398:Signal Transduction; D022502:Stress Fibers / Q000378:metabolism; D015533:Transcriptional Activation; D014471:Umbilical Veins / Q000737:chemistry / Q000378:metabolism; D020764:cdc42 GTP-Binding Protein / Q000235:genetics* / Q000378:metabolism; D020830:rac1 GTP-Binding Protein / Q000235:genetics* / Q000378:metabolism
chemical_list: D015820:Cadherins; C099972:H-cadherin; D020764:cdc42 GTP-Binding Protein; D020830:rac1 GTP-Binding Protein