Low mass cutoff evasion with q(z) value optimization in ion trap.
An ion trap is a powerful analyzer because of its high resolution, high sensitivity, and multistage mass analysis (MS(n)) capabilities. Multiple fragmentation analysis provides useful information regarding peptide sequence and biomolecular structure; however, this approach is limited by an inherent low mass cutoff (LMCO) derived from collision-induced dissociation (CID). To avoid the LMCO for application of an ion trap to iTRAQ, we optimized the q(z) value, which is a parameter that is proportional to the applied fundamental AC radio frequency voltage of a tandem mass spectrometry (MS/MS) event. Considering that many ion trap MS analyses employ CID as the MS/MS method, this method can be a practical one without any instrumental changes.
Yang
Yung-Hun
YH
School of Chemical and Biological Engineering and Interdisciplinary Program for Biochemical Engineering and Biotechnology, Institute of Bioengineering, Seoul National University, Seoul 110-744, Republic of Korea.
Lee
Kwangwon
K
Jang
Kyoung-Soon
KS
Kim
Yun-Gon
YG
Park
Sung-Hee
SH
Lee
Chang-Soo
CS
Kim
Byung-Gee
BG
D053719:Tandem Mass Spectrometry / Q000379:methods*