title: Rapid identification of biothreat and other clinically relevant bacterial species by use of universal PCR coupled with high-resolution melting analysis.
abstract: A rapid assay for eubacterial species identification is described using high-resolution melt analysis to characterize PCR products. Unique melt profiles generated from multiple hypervariable regions of the 16S rRNA gene for 100 clinically relevant bacterial pathogens, including category A and B biothreat agents and their surrogates, allowed highly specific species identification.
authors.affiliation: Johns Hopkins University, Department of Emergency Medicine, Baltimore, MD 21209, USA. syang10@jhmi.edu
authors.forename: Samuel
authors.initials: S
authors.lastname: Yang
authors.forename: Padmini
authors.initials: P
authors.lastname: Ramachandran
authors.forename: Richard
authors.initials: R
authors.lastname: Rothman
authors.forename: Yu-Hsiang
authors.initials: YH
authors.lastname: Hsieh
authors.forename: Andrew
authors.initials: A
authors.lastname: Hardick
authors.forename: Helen
authors.initials: H
authors.lastname: Won
authors.forename: Aleksandar
authors.initials: A
authors.lastname: Kecojevic
authors.forename: Joany
authors.initials: J
authors.lastname: Jackman
authors.forename: Charlotte
authors.initials: C
authors.lastname: Gaydos
mesh_terms: D001419:Bacteria / Q000302:isolation & purification*; D001424:Bacterial Infections / Q000175:diagnosis*; D023482:Bioterrorism; D004269:DNA, Bacterial / Q000235:genetics*; D004275:DNA, Ribosomal / Q000235:genetics; D004783:Environmental Microbiology; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D044366:Transition Temperature
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S