title: Epigenomic profiling indicates a role for DNA methylation in early postnatal liver development.
abstract: The question of whether DNA methylation contributes to the stabilization of gene expression patterns in differentiated mammalian tissues remains controversial. Using genome-wide methylation profiling, we screened 3757 gene promoters for changes in methylation during postnatal liver development to test the hypothesis that developmental changes in methylation and expression are temporally correlated. We identified 31 genes that gained methylation and 111 that lost methylation from embryonic day 17.5 to postnatal day 21. Promoters undergoing methylation changes in postnatal liver tended not to be associated with CpG islands. At most genes studied, developmental changes in promoter methylation were associated with expression changes, suggesting both that transcriptional inactivity attracts de novo methylation, and that transcriptional activity can override DNA methylation and successively induce developmental hypomethylation. These in vivo data clearly indicate a role for DNA methylation in mammalian differentiation, and provide the novel insight that critical windows in mammalian developmental epigenetics extend well beyond early embryonic development.
authors.affiliation: Department of Pediatrics, Baylor College of Medicine, USDA Children's Nutrition Research Center, 1100 Bates St., Ste. 5080, Houston, TX 77030, USA. waterland@bcm.edu
authors.forename: Robert A
authors.initials: RA
authors.lastname: Waterland
authors.forename: Richard
authors.initials: R
authors.lastname: Kellermayer
authors.forename: Marie-Therese
authors.initials: MT
authors.lastname: Rached
authors.forename: Nina
authors.initials: N
authors.lastname: Tatevian
authors.forename: Marcus V
authors.initials: MV
authors.lastname: Gomes
authors.forename: Jiexin
authors.initials: J
authors.lastname: Zhang
authors.forename: Li
authors.initials: L
authors.lastname: Zhang
authors.forename: Abrita
authors.initials: A
authors.lastname: Chakravarty
authors.forename: Wei
authors.initials: W
authors.lastname: Zhu
authors.forename: Eleonora
authors.initials: E
authors.lastname: Laritsky
authors.forename: Wenjuan
authors.initials: W
authors.lastname: Zhang
authors.forename: Xiaodan
authors.initials: X
authors.lastname: Wang
authors.forename: Lanlan
authors.initials: L
authors.lastname: Shen
mesh_terms: D000818:Animals; D019175:DNA Methylation; D044127:Epigenesis, Genetic; D005260:Female; D008099:Liver / Q000196:embryology / Q000254:growth & development* / Q000378:metabolism; D008297:Male; D051379:Mice / Q000196:embryology / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D011401:Promoter Regions, Genetic