title: Effects of antisense oligodeoxynucleotide to type I collagen gene on hypertrophic scars in the transplanted nude mouse model.
abstract: BACKGROUND
  Antisense nucleic acids are effective in inhibiting harmful or uncontrolled gene expression. We had previously proved that the antisense DNA to type I collagen could effectively inhibit the synthesis of collagen type I in cultured hypertrophic scar fibroblasts, suggesting a potential role in anti-scarring, but there are no published reports of its effect on scar in the transplanted nude mouse model.
  
  
  OBJECTIVE
  To investigate the effects of antisense oligodeoxynucleotide (ASODN) to type I collagen gene on hypertrophic scars in the transplanted nude mouse model and clarify the potential of ASODN for the treatment of scars.
  
  
  METHODS
  The nudemousemodel of hypertrophic scar was created and subjected to daily injections with ASODN and LipofectamineTM for 2 ,4 or 6 weeks.We then examined the scars for changes in histopathological characteristics. The effects of ASODNon type I collagen gene expression were examined by reverse transcription-polymerase chain reaction and Western blots.
  
  
  RESULTS
  The ASODN could remarkably alleviate the scar in the nude mouse model and consistently inhibit type I collagen gene expression at both the mRNA and protein levels.
  
  
  CONCLUSIONS
  ASODN was effective in downregulating type I collagen gene expression and could prove to be useful in the treatment of scars.
authors.affiliation: Department of Burns, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, China. xiejl90@sina.com
authors.forename: Julin
authors.initials: J
authors.lastname: Xie
authors.forename: Shaohai
authors.initials: S
authors.lastname: Qi
authors.forename: Jishan
authors.initials: J
authors.lastname: Yuan
authors.forename: Yinbing
authors.initials: Y
authors.lastname: Xu
authors.forename: Tianzeng
authors.initials: T
authors.lastname: Li
authors.forename: Houdong
authors.initials: H
authors.lastname: Li
authors.forename: Xusheng
authors.initials: X
authors.lastname: Liu
authors.forename: Bin
authors.initials: B
authors.lastname: Shu
authors.forename: Huizeng
authors.initials: H
authors.lastname: Liang
mesh_terms: D000818:Animals; D015153:Blotting, Western; D017439:Cicatrix, Hypertrophic / Q000235:genetics* / Q000517:prevention & control; D024042:Collagen Type I / Q000235:genetics*; D015536:Down-Regulation; D004797:Enzyme-Linked Immunosorbent Assay; D015870:Gene Expression / Q000187:drug effects*; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D020319:Oligodeoxyribonucleotides, Antisense / Q000494:pharmacology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016038:Skin Transplantation
chemical_list: D024042:Collagen Type I; D020319:Oligodeoxyribonucleotides, Antisense