Effect of inducers on the decolorization and biodegradation of textile azo dye Navy blue 2GL by Bacillus sp. VUS.
Bacillus sp. VUS decolorized azo dye Navy blue 2GL in 48 h at static anoxic condition in yeast extract medium, whereas it took only 18 h for the decolorization in presence of CaCl(2). Different inducers played role in the decolorization of Navy blue 2GL. CaCl(2) found to be the most effective inducer among all inducers tested. The activity of enzymes like lignin peroxidase, laccase and reductases viz. NADH-DCIP, azo and riboflavin induced during decolorization represents their role in the biodegradation. Extracellular LiP and intracellular laccase activity induced with CaCl(2). Yeast extract was best medium for faster decolorization than other media. UV-vis spectrophotometer analysis and visual examinations showed decolorization of dye. High performance liquid chromatography, Fourier transforms infrared spectroscopy showed degradation of dye. Gas Chromatography-Mass Spectroscopy revealed formation of 4-Amino-3-(2-bromo-4, 6-dinitro-phenylazo)-phenol and acetic acid 2-(-acetoxy-ethylamino)-ethyl ester as final products. Bacillus sp. VUS also decolorized synthetic effluent. Phytotoxicity study showed detoxification of Navy blue 2GL.
Dawkar
Vishal V
VV
Department of Biochemistry, Shivaji University, Kolhapur 416004, India. vvdawkar@yahoo.co.in
Jadhav
Umesh U
UU
Ghodake
Gajanan S
GS
Govindwar
Sanjay P
SP
D001391:Azo Compounds / Q000378:metabolism*; D001407:Bacillus / Q000187:drug effects / Q000201:enzymology / Q000254:growth & development / Q000378:metabolism*; D001673:Biodegradation, Environmental / Q000187:drug effects; D018533:Biomass; D003116:Color; D004396:Coloring Agents / Q000378:metabolism*; D003470:Culture Media; D006861:Hydrogen Peroxide / Q000494:pharmacology; D007700:Kinetics; D042845:Laccase / Q000378:metabolism; D014442:Monophenol Monooxygenase / Q000378:metabolism; D009195:Peroxidase / Q000378:metabolism; D010655:Phenylenediamines / Q000378:metabolism*; D045868:Sorghum / Q000187:drug effects; D013056:Spectrophotometry, Ultraviolet; D017550:Spectroscopy, Fourier Transform Infrared; D013784:Textiles; D013997:Time Factors; D014908:Triticum / Q000187:drug effects
D001391:Azo Compounds; D004396:Coloring Agents; D003470:Culture Media; D010655:Phenylenediamines; C546225:navy blue 2GL; D006861:Hydrogen Peroxide; D042845:Laccase; D009195:Peroxidase; D014442:Monophenol Monooxygenase