title: Proposal of a low-cost protocol for colorimetric semi-quantification of secretory phospholipase by Candida albicans grown in planktonic and biofilm phases.
abstract: Biofilms are aggregates of microorganisms living in multilayered structures inside polymeric matrices onto surfaces. These biofilms may subvert the physiological properties of adjacent tissues causing morphofunctional failure. Many studies have shown that the expression of virulence attributes is maximized when microbes form such communities. This study evaluated the differential phospholipasic activity of Candida albicans SC5314 grown in planktonic phase and in biofilm. We propose two distinct protocols for the colorimetric evaluation of phosphatidylcholine hydrolysis in neutral and acidic conditions. The results showed that both protocols are suitable for the proposed intention and that 72 h-old planktonic cultures of C. albicans SC5314 secrete higher quantities of neutral (6.42-fold) and acidic (3.85-fold) phospholipases than biofilms.
authors.affiliation: Laboratory of Stomatology, Faculty of Dentistry, The Pontifical Catholic University of Paraná, Rua Imaculada Conceição 1155, Curitiba, Brazil.
authors.forename: Lisa
authors.initials: L
authors.lastname: Taniguchi
authors.forename: Berenice
authors.initials: B
authors.lastname: de Fátima Faria
authors.forename: Rosimeire Takaki
authors.initials: RT
authors.lastname: Rosa
authors.forename: Alessandra
authors.initials: A
authors.lastname: de Paula E Carvalho
authors.forename: Lauren Christine
authors.initials: LC
authors.lastname: Gursky
authors.forename: Selene Lobo
authors.initials: SL
authors.lastname: Elifio-Esposito
authors.forename: Nipuna
authors.initials: N
authors.lastname: Parahitiyawa
authors.forename: Lakshman Perera
authors.initials: LP
authors.lastname: Samaranayake
authors.forename: Edvaldo Antonio Ribeiro
authors.initials: EA
authors.lastname: Rosa
mesh_terms: D018441:Biofilms; D002176:Candida albicans / Q000201:enzymology* / Q000502:physiology; D003124:Colorimetry / Q000191:economics* / Q000379:methods*; D005656:Fungal Proteins / Q000378:metabolism*; D010713:Phosphatidylcholines / Q000378:metabolism; D010740:Phospholipases / Q000378:metabolism*
chemical_list: D005656:Fungal Proteins; D010713:Phosphatidylcholines; D010740:Phospholipases