title: Aberrant gene expression profile in a mouse model of endometriosis mirrors that observed in women.
abstract: OBJECTIVE
  To define the altered gene expression profile of endometriotic lesions in a mouse model of surgically induced endometriosis.
  
  
  METHODS
  Autologous experimental mouse model.
  
  
  METHODS
  Medical school department.
  
  
  METHODS
  Adult C57Bl6 mice.
  
  
  METHODS
  Endometriosis was surgically induced by autotransplantation of uterine tissue to the intestinal mesentery. Endometriotic lesions and eutopic uteri were recovered at 3 or 29 days after induction.
  
  
  METHODS
  Altered gene expression was measured in the endometriotic lesion relative to the eutopic uterus by genome-wide complementary DNA microarray analysis and was confirmed by real-time reverse transcriptase-polymerase chain reaction for six genes. Relevant categories of altered genes were identified using gene ontology analysis to determine groups of genes enriched for altered expression.
  
  
  RESULTS
  The expression of 479 and 114 genes was altered in the endometriotic lesion compared with the eutopic uterus at 3 or 29 days after induction, respectively. Gene ontology enrichment analysis revealed that genes associated with the extracellular matrix, cell adhesions, immune function, cell growth, and angiogenesis were altered in the endometriotic lesion compared with the eutopic uterus.
  
  
  CONCLUSIONS
  According to gene expression analysis, the mouse model of surgically induced endometriosis is a good model for studying the pathophysiology and treatment of endometriosis.
authors.affiliation: Department of Obstetrics, Gynecology and Women's Health, University of Missouri, Columbia, Missouri 65212, USA.
authors.forename: Katherine E
authors.initials: KE
authors.lastname: Pelch
authors.forename: Amy L
authors.initials: AL
authors.lastname: Schroder
authors.forename: Paul A
authors.initials: PA
authors.lastname: Kimball
authors.forename: Kathy L
authors.initials: KL
authors.lastname: Sharpe-Timms
authors.forename: J Wade
authors.initials: JW
authors.lastname: Davis
authors.forename: Susan C
authors.initials: SC
authors.lastname: Nagel
mesh_terms: D000818:Animals; D049109:Cell Proliferation; D004195:Disease Models, Animal; D004715:Endometriosis / Q000235:genetics* / Q000473:pathology; D005260:Female; D020869:Gene Expression Profiling / Q000379:methods*; D005786:Gene Expression Regulation; D006801:Humans; D051379:Mice; D008810:Mice, Inbred C57BL; D020411:Oligonucleotide Array Sequence Analysis; D015203:Reproducibility of Results; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013045:Species Specificity; D013997:Time Factors; D014182:Transplantation, Autologous; D014599:Uterus / Q000637:transplantation