IL-17 potentiates neuronal injury induced by oxygen-glucose deprivation and affects neuronal IL-17 receptor expression.
Interleukin-17 (IL-17) is active in a variety of brain injuries, including ischemia. The objective of this study was to test the hypothesis that IL-17 potentiates neuronal injury after stroke. Increased expression of IL-17 and IL-17 receptor (IL-17R) in serum and cortex was evaluated by ELISA, RT-PCR and immunohistochemistry. In the in vitro model of oxygen-glucose deprivation (OGD), IL-17 showed a dose-dependent effect in promoting neuronal injury through IL-17-IL-17R combination which can be blocked by IL-17R/Fc chimera. Our results demonstrated the up-regulation of IL-17 and IL-17R following permanent middle cerebral artery occlusion and suggested that they contributed to stroke outcome.
Wang
Dan-dan
DD
Department of Neurobiology, Harbin Medical University, Heilongjiang Provincial Key Laboratory of Neurobiology, 157 Bao Jian Road, Harbin, China, 150081.
Zhao
Yan-feng
YF
Wang
Guang-you
GY
Sun
Bo
B
Kong
Qing-fei
QF
Zhao
Kai
K
Zhang
Yao
Y
Wang
Jing-hua
JH
Liu
Yu-mei
YM
Mu
Li-li
LL
Wang
De-sheng
DS
Li
Hu-lun
HL
D000818:Animals; D017209:Apoptosis; D002545:Brain Ischemia / Q000473:pathology*; D015687:Cell Hypoxia; D002478:Cells, Cultured; D005947:Glucose / Q000378:metabolism*; D006624:Hippocampus / Q000473:pathology; D006801:Humans; D020381:Interleukin-17 / Q000032:analysis / Q000235:genetics / Q000502:physiology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009474:Neurons / Q000473:pathology*; D053722:Receptors, Interleukin-17 / Q000032:analysis / Q000235:genetics / Q000502:physiology*
D020381:Interleukin-17; D053722:Receptors, Interleukin-17; D005947:Glucose