Effect of 2-octyl-cyanoacrylate on ischemic anastomosis of the left colon.
BACKGROUND
2-octyl-cyanoacrylate may be a good alternative in ischemic colon anastomosis without impairment of tissue perfusion.


METHODS
Forty male Wistar albino rats were randomized into four experimental groups (n = 10 in each group). In group 1 (control), a well-perfused left colonic segment was transected, and free ends were anastomosed by propylene sutures. In groups 2, 3, and 4, the animals underwent a standardized surgical procedure to induce ischemic left colon anastomosis. In group 2, an end-to-end anastomosis was created using six interrupted 6-0 polypropylene sutures between ischemic edges. In group 3, after approximating the mesenteric and antimesenteric edges of the anastomosis with two 6-0 polypropylene sutures, 2-octyl-cyanoacrylate was applied between the edges. In group 4, anastomosis was created by the same technique as described in group 2, and additionally 2-octyl-cyanoacrylate was applied on suture line. Rats were killed on day 4 following operation. Anastomotic integrity, intraperitoneal adhesion scores, anastomotic bursting pressures, and tissue hydroxyproline levels were recorded. Histopathological examination of the anastomosis was also performed.


RESULTS
There were no statistically significant differences among groups with respect to anastomotic integrity (p =.142). The mean bursting pressure values were 81.1 +/- 23.83, 43.3 +/- 26.06, 15.8 +/- 11.3, and 17.6 +/- 18.02 in groups 1, 2, 3, and 4, respectively. There were no statistically significant differences among groups 2, 3, and 4 with respect to tissue hydroxyproline levels and bursting pressure levels. The highest adhesion scores were observed in groups 3 and 4.


CONCLUSIONS
2-octyl cyanoacrylate does not have additional advantages in the healing of experimental ischemic colon anastomosis.
Irkorucu
Oktay
O
Department of General Surgery, Zonguldak Karaelmas University, Medical School Zonguldak, Turkey. oktaytip@yahoo.com
Ucan
Bulent Hamdi
BH
Cakmak
Guldeniz Karadeniz
GK
Tascilar
Oge
O
Emre
Ali Ugur
AU
Ofluoglu
Ebru
E
Bahadir
Burak
B
Karakaya
Kemal
K
Demirtas
Canan
C
Pasaoglu
Hatice
H
Ankarali
Handan
H
Comert
Mustafa
M
D000714:Anastomosis, Surgical / Q000379:methods; D000818:Animals; D015415:Biomarkers; D003106:Colon / Q000098:blood supply* / Q000737:chemistry / Q000473:pathology / Q000601:surgery; D019245:Compressive Strength; D003487:Cyanoacrylates / Q000627:therapeutic use*; D006909:Hydroxyproline / Q000032:analysis; D007511:Ischemia / Q000601:surgery*; D008297:Male; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D013529:Surgical Wound Dehiscence / Q000517:prevention & control; D013536:Suture Techniques; D014014:Tissue Adhesives / Q000627:therapeutic use*; D014945:Wound Healing
D015415:Biomarkers; D003487:Cyanoacrylates; D014014:Tissue Adhesives; C100832:octyl 2-cyanoacrylate; D006909:Hydroxyproline