Toxic effects of Cu(2+) on growth, nutrition, root morphology, and distribution of Cu in roots of Sabi grass.
Sabi grass (Urochloa mosambicensis (Hack.) Dandy) (a C4 species of Poaceae) is commonly used to revegetate disturbed sites in low-rainfall environments, but comparatively little is known regarding copper (Cu) toxicity in this species. A dilute nutrient solution culture experiment was conducted for 10 d to examine the effects of elevated Cu(2+) activities ({Cu(2+)}) on the growth of Sabi grass. Growth was inhibited by high Cu in solution, with a 50% reduction in the relative fresh mass occurring at 1.0 microM {Cu(2+)} for the roots and 1.2 microM {Cu(2+)} for the shoots. In solutions containing 1.2-1.9 microM {Cu(2+)}, many of the roots ruptured due to the tearing and separation of the rhizodermis and outer cortex from the underlying tissues. Transmission electron microscopy revealed that Cu-rich deposits were found to accumulate predominantly within vacuoles. Due to limited translocation of Cu from the roots to the shoots, phytotoxicity is likely to be more of a problem in remediation of Cu-toxic sites than is Cu toxicity of fauna consuming the above-ground biomass.
Kopittke
P M
PM
School of Land, Crop and Food Sciences, The University of Queensland, St. Lucia, Queensland 4072, Australia. p.kopittke@uq.edu.au
Asher
C J
CJ
Blamey
F P C
FP
Menzies
N W
NW
D003300:Copper / Q000493:pharmacokinetics / Q000633:toxicity*; D004785:Environmental Pollutants / Q000493:pharmacokinetics / Q000633:toxicity*; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D008954:Models, Biological; D018517:Plant Roots / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism / Q000648:ultrastructure; D018520:Plant Shoots / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism / Q000648:ultrastructure; D006109:Poaceae / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism / Q000648:ultrastructure; D013054:Spectrophotometry, Atomic
D004785:Environmental Pollutants; D003300:Copper; C029892:cupric chloride