title: Functionally relevant decreases in activatory receptor expression on NK cells are associated with pulmonary tuberculosis in vivo and persist after successful treatment.
abstract: Correlates for the initiation of Mycobacterium tuberculosis hominis (Mth) replication from latency are needed in order to improve Mth control. In order to analyze if perturbations of peripheral NK cells may be associated with exit from Mth latency, sequential patients with newly diagnosed lung tuberculosis (TB) were studied. Peripheral NK cells were analyzed by cytofluorometry, in vitro culture and functional assays. At the onset of lung TB, imbalances in NK cell subsets were evident. Decreased CD56(bright)CD16(+/-) subsets with significantly compromised NKp30 and NKp46 expression and with specifically decreased gamma-IFN production upon triggering were evident. These features were not completely restored when purified NK cells were cultured in vitro. Culture supplementation with alpha-IFN increased only NKp30 expression in TB and healthy donors. Extensive peripheral NK cell triggering was evident in these patients, as shown by the expression of NK cell activation markers and of the lymph node-homing chemokine receptor CCR7 on CD16(+) CD56(dull) cells. Significant persistence of decreased NKp30 and NKp46 after successful treatment with a standard four-drug regimen was detected after full recovery. NK cell function is deeply affected in patients at the onset of pulmonary TB. The involvement of multiple activatory receptors may provide a relevant contribution to the spread of mycobacteria exiting from latency.
authors.affiliation: Centro di Eccellenza per la Ricerca Biomedica, Università di Genova, Genova, Italy.
authors.forename: Federica
authors.initials: F
authors.lastname: Bozzano
authors.forename: Paola
authors.initials: P
authors.lastname: Costa
authors.forename: Giovanni
authors.initials: G
authors.lastname: Passalacqua
authors.forename: Ferdinando
authors.initials: F
authors.lastname: Dodi
authors.forename: Silvia
authors.initials: S
authors.lastname: Ravera
authors.forename: Gabriella
authors.initials: G
authors.lastname: Pagano
authors.forename: Giorgio W
authors.initials: GW
authors.lastname: Canonica
authors.forename: Lorenzo
authors.initials: L
authors.lastname: Moretta
authors.forename: Andrea
authors.initials: A
authors.lastname: De Maria
mesh_terms: D006684:HLA-DR Antigens / Q000276:immunology / Q000378:metabolism; D006801:Humans; D016898:Interferon-alpha / Q000494:pharmacology; D007371:Interferon-gamma / Q000096:biosynthesis / Q000276:immunology; D007694:Killer Cells, Natural / Q000187:drug effects / Q000276:immunology* / Q000382:microbiology; D009169:Mycobacterium tuberculosis; D055627:Natural Cytotoxicity Triggering Receptor 1 / Q000276:immunology / Q000378:metabolism; D055628:Natural Cytotoxicity Triggering Receptor 3 / Q000276:immunology / Q000378:metabolism; D054400:Receptors, CCR7 / Q000276:immunology / Q000378:metabolism; D014397:Tuberculosis, Pulmonary / Q000276:immunology*
chemical_list: C515370:CCR7 protein, human; D006684:HLA-DR Antigens; D016898:Interferon-alpha; C112260:NCR1 protein, human; C431710:NCR3 protein, human; D055627:Natural Cytotoxicity Triggering Receptor 1; D055628:Natural Cytotoxicity Triggering Receptor 3; D054400:Receptors, CCR7; D007371:Interferon-gamma