title: Association between omental adipose tissue macrophages and liver histopathology in morbid obesity: influence of glycemic status.
abstract: OBJECTIVE
  Recently we showed that macrophage accumulation in omental adipose tissue is associated with liver fibro-inflammation in morbidly obese subjects. Here, we evaluated the influence of glycemic status and extended the analysis to the spectrum of obesity-linked liver damage.
  
  
  METHODS
  Liver biopsies, subcutaneous and omental adipose tissue were collected in 132 obese subjects during gastric bypass surgery. HAM56+ adipose tissue macrophages were counted in subjects classified by liver histopathology and by their degree of insulin resistance.
  
  
  RESULTS
  In the whole population, the number of omental macrophages increased with the score of steatosis, the non-alcoholic fatty liver disease activity score, the stage of fibrosis and with fibro-inflammation index. None of these relationships were significant with subcutaneous macrophage count. In insulin-sensitive participants, omental macrophages accumulation was higher in subjects with high indexes of fibro-inflammation (p=0.012 vs. low indexes). In insulin-resistant including type 2 diabetic participants, omental macrophage count was higher both in subjects with high scores of steatosis and in subjects with high indexes of fibro-inflammation (p<0.05 vs. low scores).
  
  
  CONCLUSIONS
  Macrophage accumulation in omental adipose tissue is associated with aggravated steatosis and fibro-inflammation in insulin-resistant obese subjects independently of altered glycemic status.
authors.affiliation: INSERM, U872, Eq7, 15 Rue de l'Ecole de Medicine, 75007 Paris, France.
authors.forename: Joan
authors.initials: J
authors.lastname: Tordjman
authors.forename: Christine
authors.initials: C
authors.lastname: Poitou
authors.forename: Danielle
authors.initials: D
authors.lastname: Hugol
authors.forename: Jean-Luc
authors.initials: JL
authors.lastname: Bouillot
authors.forename: Arnaud
authors.initials: A
authors.lastname: Basdevant
authors.forename: Pierre
authors.initials: P
authors.lastname: Bedossa
authors.forename: Michèle
authors.initials: M
authors.lastname: Guerre-Millo
authors.forename: Karine
authors.initials: K
authors.lastname: Clement
mesh_terms: D050153:Abdominal Fat / Q000473:pathology*; D000328:Adult; D001786:Blood Glucose / Q000378:metabolism*; D002452:Cell Count; D003924:Diabetes Mellitus, Type 2 / Q000097:blood / Q000150:complications / Q000473:pathology; D005234:Fatty Liver / Q000097:blood / Q000150:complications / Q000473:pathology; D005260:Female; D006801:Humans; D007333:Insulin Resistance; D008099:Liver / Q000473:pathology*; D008103:Liver Cirrhosis / Q000097:blood / Q000150:complications / Q000473:pathology; D008264:Macrophages / Q000473:pathology*; D008297:Male; D009767:Obesity, Morbid / Q000097:blood* / Q000150:complications / Q000473:pathology*; D009852:Omentum / Q000473:pathology*
chemical_list: D001786:Blood Glucose