title: In vitro activities of three synthetic peptides derived from epinecidin-1 and an anti-lipopolysaccharide factor against Propionibacterium acnes, Candida albicans, and Trichomonas vaginalis.
abstract: The synthetic epinecidin-1(22-42) peptide was derived from positions 22-42 of Epinephelus coioides epinecidin-1. The synthetic SALF(55-76) cyclic peptide (csSALF(55-76)) and SALF(55-76) linear peptide (lsSALF(55-76)) contained sequences from positions 55 to 76 of the Penaeus monodon anti-lipopolysaccharide factor (ALF), respectively. We studied the in vitro activities of epinecidin-1(22-42), csSALF(55-76), and lsSALF(55-76) against Propionibacterium acnes, Candida albicans, and Trichomonas vaginalis. The minimum inhibitory concentrations (MICs) of epinecidin-1(22-42) for the test pathogen strains ranged 12.5-200 microg/ml, those of csSALF(55-76) ranged 100-200 microg/ml, and those of lsSALF(55-76) ranged 25-200 microg/ml. epinecidin-1(22-42) exhibited cytotoxicity towards P. acnes, C. albicans, and T. vaginalis (one strain of which was a metronidazole-resistant strain, while the other strain was not), suggesting that epinecidin-1 functions like a lytic peptide. Similar cytotoxicity was identified against T. vaginalis treated with the csSALF(55-76) and lsSALF(55-76) peptides. The antimicrobial activities of these peptides were confirmed by scanning electron microscopy (SEM), transmission electron microscopy (TEM), a viable cell count assay, and flow cytometric analysis. TEM and SEM examinations of T. vaginalis treated with these three peptides showed that severe swelling preceded cell death and breakage of the outer membrane, and the intracellular inclusion was found to have effluxed extracellularly. This phenomenon was also found with epinecidin-1(22-42) treatment of P. acnes and C. albicans. Our results suggest that the epinecidin-1(22-42), csSALF(55-76), and lsSALF(55-76) peptides may be good candidates for treating trichomoniasis, and epinecidin-1(22-42) may have potential as a drug supporting therapy for acne and candidiasis.
authors.affiliation: Department of Aquaculture, National Taiwan Ocean University, Keelung, Taiwan.
authors.forename: Chieh-Yu
authors.initials: CY
authors.lastname: Pan
authors.forename: Jyh-Yih
authors.initials: JY
authors.lastname: Chen
authors.forename: Tai-Lang
authors.initials: TL
authors.lastname: Lin
authors.forename: Cheng-Hui
authors.initials: CH
authors.lastname: Lin
mesh_terms: D000818:Animals; D023181:Antimicrobial Cationic Peptides / Q000737:chemistry*; D002176:Candida albicans / Q000187:drug effects*; D029941:Fish Proteins / Q000737:chemistry*; D008070:Lipopolysaccharides / Q000037:antagonists & inhibitors*; D008826:Microbial Sensitivity Tests; D010446:Peptide Fragments / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology*; D011425:Propionibacterium acnes / Q000187:drug effects*; D014246:Trichomonas vaginalis / Q000187:drug effects*
chemical_list: D023181:Antimicrobial Cationic Peptides; D029941:Fish Proteins; D008070:Lipopolysaccharides; D010446:Peptide Fragments; C521392:epinecidin-1, Epinephelus coioides