title: Intramuscular delivery of a cholera DNA vaccine primes both systemic and mucosal protective antibody responses against cholera.
abstract: Cholera is a potentially lethal diarrhea disease caused by the gram-negative bacterium Vibrio cholerae. The need for an effective cholera vaccine is clearly indicated but the challenges of eliciting both systemic and mucosal immune responses remains a significant challenge. In the current report, we discovered that a DNA vaccine expressing a protective cholera antigen, cholera toxin B subunit (CTB), delivered parenterally can elicit both systemic and mucosal anti-CTB antibody responses in mice. The priming effect by DNA immunization was demonstrated by higher mucosal antibody responses following one boost with the inactivated cholera vaccine (KWC-B) delivered orally when compared to the twice oral administration of KWC-B alone. This finding indicates that DNA vaccines delivered parenterally are effective in eliciting mucosal protective immune responses--a unique advantage for DNA vaccination that has not yet been well realized and should bring value to the development of novel vaccination approaches against mucosally transmitted diseases.
authors.affiliation: Department of Infectious Diseases, First Affiliated Hospital, Nanjing Medical University, Nanjing 210029, China.
authors.forename: Guifang
authors.initials: G
authors.lastname: Xu
authors.forename: Shixia
authors.initials: S
authors.lastname: Wang
authors.forename: Ling
authors.initials: L
authors.lastname: Zhuang
authors.forename: Anthony
authors.initials: A
authors.lastname: Hackett
authors.forename: Ling
authors.initials: L
authors.lastname: Gu
authors.forename: Lu
authors.initials: L
authors.lastname: Zhang
authors.forename: Chunhua
authors.initials: C
authors.lastname: Zhang
authors.forename: Hua
authors.initials: H
authors.lastname: Wang
authors.forename: Zuhu
authors.initials: Z
authors.lastname: Huang
authors.forename: Shan
authors.initials: S
authors.lastname: Lu
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis; D000992:Antitoxins / Q000032:analysis; D002771:Cholera / Q000276:immunology* / Q000517:prevention & control; D002772:Cholera Toxin / Q000008:administration & dosage* / Q000276:immunology*; D022121:Cholera Vaccines / Q000008:administration & dosage* / Q000276:immunology*; D018928:Immunity, Mucosal; D007273:Injections, Intramuscular; D051379:Mice; D008807:Mice, Inbred BALB C; D011817:Rabbits; D019444:Vaccines, DNA / Q000008:administration & dosage* / Q000276:immunology*
chemical_list: D000907:Antibodies, Bacterial; D000992:Antitoxins; D022121:Cholera Vaccines; D019444:Vaccines, DNA; D002772:Cholera Toxin