Maize AKINbetagamma dimerizes through the KIS/CBM domain and assembles into SnRK1 complexes.
The SNF1/AMPK/SnRK1 complex is an intracellular energy sensor composed of three types of subunits: the SnRK1 kinase and two regulatory, non-catalytic subunits (designated beta and gamma). We have previously described an atypical plant gamma-subunit, AKINbetagamma, which contains an N-terminal tail similar to the so-called KIS domain normally present in beta-subunits. However, it is not known whether AKINbetagamma normally associates with endogenous SnRK1 complexes in vivo, nor how its unique domain structure might contribute to SnRK1 function. Here, we present evidence that maize AKINbetagamma is an integral component of active SnRK1 complexes in plant cells. Using complementary methodological approaches, we also show that AKINbetagamma associates through homomeric interactions mediated by both, the gamma- and, unexpectedly, the KIS/CBM domain.
López-Paz
Cristina
C
Department of Molecular Genetics, Centre de Recerca en Agrigenomica (CRAG), CSIC-IRTA-UAB, Barcelona, Spain.
Vilela
Belmiro
B
Riera
Marta
M
Pagès
Montserrat
M
Lumbreras
Victoria
V
D000595:Amino Acid Sequence; D017360:Arabidopsis; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D050260:Carbohydrate Metabolism; D002478:Cells, Cultured; D019281:Dimerization; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D019697:Onions; D010940:Plant Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D030821:Plants, Genetically Modified; D017346:Protein Serine-Threonine Kinases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D017434:Protein Structure, Tertiary; D021122:Protein Subunits; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D003313:Zea mays / Q000201:enzymology* / Q000235:genetics
D001426:Bacterial Proteins; D008164:Luminescent Proteins; D010940:Plant Proteins; D021122:Protein Subunits; D011993:Recombinant Fusion Proteins; C054562:yellow fluorescent protein, Bacteria; C071570:SNF1-related protein kinases; D017346:Protein Serine-Threonine Kinases