title: [Primry research of separation and culture of adult hepatocytes].
abstract: OBJECTIVE
  To explore the separation and culture method of adult hepatocytes.
  
  
  METHODS
  The isolated adult hepatocytes were cultivated by RPMI 1640 medium at 37 degrees C in vitro. The characteristics of the growing hepatocytes were observed. Their synthesis of urea was detected. The transformation efficiency and density's change of lidocaine were analyzed.
  
  
  RESULTS
  Hepatocytes were successful separated from adult liver. And they were cultivated in common condition and hollow fiber reactor. The functional capacity of hepatocytes was for lidocaine metabolism and urea excretion.
  
  
  CONCLUSIONS
  The adult hepatocytes have been successful separated from liver. And they can be cultivated in common condition and hollow fiber reactor. And it could provide a great quantity and high activity of hepatocytes for bioartificial liver.
authors.affiliation: Department of Virology, Institute of Infectious Diseases, No. 302 Hospital, Beijing 100039, China.
authors.forename: Hong-ling
authors.initials: HL
authors.lastname: Liu
authors.forename: Shao-jie
authors.initials: SJ
authors.lastname: Xin
authors.forename: Shao-li
authors.initials: SL
authors.lastname: You
authors.forename: Yan
authors.initials: Y
authors.lastname: Hu
authors.forename: Yi-hui
authors.initials: YH
authors.lastname: Rong
authors.forename: Lin
authors.initials: L
authors.lastname: Zhou
authors.forename: Yong-ping
authors.initials: YP
authors.lastname: Yang
authors.forename: Zhi-wei
authors.initials: ZW
authors.lastname: Li
authors.forename: Zhen-wen
authors.initials: ZW
authors.lastname: Liu
authors.forename: Pan-yong
authors.initials: PY
authors.lastname: Mao
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques; D002478:Cells, Cultured; D022781:Hepatocytes / Q000378:metabolism*; D006801:Humans; D008099:Liver / Q000166:cytology*; D019164:Liver, Artificial / Q000639:trends*; D012106:Research