The nanodisc: a novel tool for membrane protein studies.
A major challenge in the research on membrane-anchored and integral membrane protein complexes is to obtain these in a functionally active, water-soluble, and monodisperse form. This requires the incorporation of the membrane proteins into a native-like membrane or detergent micelle that mimics the properties of the original biological membrane. However, solubilization in detergents or reconstitution in liposomes or supported monolayers sometimes suffers from loss of activity and problematic analyses due to heterogeneity and aggregation. A developing technology termed nanodiscs exploits discoidal phospholipid bilayers encircled by a stabilizing amphipatic helical membrane scaffold protein to reconstitute membranes with integral proteins. After reconstitution, the membrane nanodisc is soluble, stable, and monodisperse. In the present review, we outline the biological inspiration for nanodiscs as discoidal high-density lipoproteins, the assembly and handling of nanodiscs, and finally their diverse biochemical applications. In our view, major advantages of nanodisc technology for integral membrane proteins is homogeneity, control of oligomerization state, access to both sides of the membrane, and control of lipids in the local membrane environment of the integral protein.
Borch
Jonas
J
Department of Biochemistry and Molecular Biology, University of Southern Denmark, Campusvej 55, DK-5230 Odense M, Denmark. jonasb@bmb.sdu.dk
Hamann
Thomas
T
D016632:Apolipoprotein A-I / Q000737:chemistry; D008051:Lipid Bilayers / Q000737:chemistry; D008075:Lipoproteins, HDL / Q000737:chemistry; D008081:Liposomes; D008565:Membrane Proteins / Q000737:chemistry*; D026901:Membrane Transport Proteins / Q000737:chemistry; D008958:Models, Molecular; D049329:Nanostructures / Q000737:chemistry*; D010743:Phospholipids / Q000737:chemistry; D012995:Solubility
D016632:Apolipoprotein A-I; D008051:Lipid Bilayers; D008075:Lipoproteins, HDL; D008081:Liposomes; D008565:Membrane Proteins; D026901:Membrane Transport Proteins; D010743:Phospholipids