[Development of a multiplex PCR-suspension array for simultaneous detection of five bioterrorism bacteria].
OBJECTIVE
To develop a rapid, high-throughput screening method of gene suspension array technique to simultaneously detect five bioterrorism bacteria: Bacillus anthracis, Francisella tularensis, Yersinia pestis, Brucella spp. and Burkholderia pseudomallei.


METHODS
Highly validated specific primers were used to amplify diagnostic regions unique to each pathogen. Biotin labelled PCR products were hybridized to corresponding probes coupling on the unique sets of fluorescent beads. The hybridized beads were processed through the Bio-plex, which identified the presence of PCR products.


RESULTS
Multiplex PCR-suspension array can detect five bioterrorism bacteria correctly with high specificity and high sensitivity, the results suggest the utility of suspension array system for high-throughput screening of bioterrorism samples.


CONCLUSIONS
A multiplex PCR-suspension array for rapid detection of five bioterrorism bacteria was established.
Wen
Hai-yan
HY
China Academy of Inspection and Quarantin, Beijing 100025, China.
Wang
Jing
J
Liu
Heng-chuan
HC
Yang
Yu
Y
Hu
Kong-xin
KX
Sun
Xiao-hong
XH
D001408:Bacillus anthracis / Q000235:genetics / Q000302:isolation & purification*; D023482:Bioterrorism; D002002:Brucella / Q000235:genetics / Q000302:isolation & purification; D016957:Burkholderia pseudomallei / Q000235:genetics / Q000302:isolation & purification; D005604:Francisella tularensis / Q000235:genetics / Q000302:isolation & purification*; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D015010:Yersinia pestis / Q000235:genetics / Q000302:isolation & purification*