title: Histocompatibility assessment in the chicken colonies of the RIR-Y8/NU, YL, WL-G, and BL-E closed for 28-48 years.
abstract: Histocompatibility was assessed in the RIR-Y8/NU, BL-E, YL, and WL-G chicken closed colonies by hemagglutination test using anti-red blood cell (RBC) antibodies (HT), skin transplantation test (STT), and formation of isohemagglutinins (FIHs) during STT. The YL individuals all showed the survival of skingrafts for more than 17 days with no FIHs in STT and no RBC antigenic variations in HT, indicating a histocompatible nature together with high homogeneity at serological loci. The BL-E as well as WL-G closed colonies were also found to be histocompatible in the STT with no FIHs, although the HT showed heterogeneities at serological locus/loci other than the B and C blood group loci which have significant effects on histocompatibility or FIHs in chicken. In the RIR-Y8/NU closed colonies, one individual in 6 reciprocal combinations of the STT showed early skingraft rejection with positive FIHs caused by different B locus alleles, and the HT suggested relatively high heterogeneities at the other serological loci too. The closed colonies of YL, BL-E, and WL-G will be useful avian materials for transplantation or related experiments, but RIR-Y8/NU needs further pedigree selection for serological homogeneity.
authors.affiliation: Laboratory of Animal Genetics, Graduate School of Bioagricultural Sciences, Nagoya University, Chikusa, Nagoya, Japan.
authors.forename: Marcos B
authors.initials: MB
authors.lastname: Valdez
authors.forename: Keiji
authors.initials: K
authors.lastname: Kinoshita
authors.forename: Makoto
authors.initials: M
authors.lastname: Mizutani
authors.forename: Akira
authors.initials: A
authors.lastname: Fujiwara
authors.forename: Hajime
authors.initials: H
authors.lastname: Yazawa
authors.forename: Takahiro
authors.initials: T
authors.lastname: Yamagata
authors.forename: Kiyoshi
authors.initials: K
authors.lastname: Shimada
authors.forename: Takao
authors.initials: T
authors.lastname: Namikawa
mesh_terms: D000818:Animals; D002645:Chickens / Q000235:genetics* / Q000276:immunology; D005260:Female; D006386:Hemagglutination Tests; D006649:Histocompatibility Antigens / Q000235:genetics / Q000276:immunology; D006650:Histocompatibility Testing; D007178:Inbreeding; D008285:Major Histocompatibility Complex / Q000235:genetics* / Q000276:immunology; D008297:Male; D016038:Skin Transplantation / Q000276:immunology
chemical_list: D006649:Histocompatibility Antigens