Adhesion of human U937 monocytes to nitrogen-rich organic thin films: novel insights into the mechanism of cellular adhesion.
We present a two-fold study designed to elucidate the adhesion mechanism of human U937 monocytes on novel N-rich thin films deposited by plasma- and VUV photo-polymerisation, so-called "PVP:N" materials. It is shown that there exist sharply-defined ("critical") surface-chemical conditions that are necessary to induce cell adhesion. By comparing the film chemistries at the "critical" conditions, we demonstrate the dominant role of primary amines in the cell adhesion mechanism. Quantitative real-time RT-PCR experiments using U937 cells that had adhered to PVP:N materials for up to 24 h are presented. The adhesion induces a transient expression of cytokines, markers of macrophage activation, as well as a more sustained expression of PPAR gamma and ICAM-I.
Girard-Lauriault
Pierre-Luc
PL
Groupe de Recherche en Physique et Technologie des Couches Minces (GCM) and Department of Engineering Physics, Ecole Polytechnique de Montréal, P.O. Box 6079, Station Centre-Ville, Montréal QC H3C 3A7, Canada.
Truica-Marasescu
Florina
F
Petit
Alain
A
Wang
Hong T
HT
Desjardins
Patrick
P
Antoniou
John
J
Mwale
Fackson
F
Wertheimer
Michael R
MR
D000818:Animals; D002448:Cell Adhesion / Q000502:physiology*; D002460:Cell Line; D016207:Cytokines / Q000235:genetics / Q000378:metabolism; D051766:Early Growth Response Protein 1 / Q000235:genetics / Q000378:metabolism; D055664:Electrochemical Techniques / Q000295:instrumentation / Q000379:methods; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000235:genetics / Q000378:metabolism; D009000:Monocytes / Q000166:cytology / Q000502:physiology*; D009584:Nitrogen / Q000737:chemistry*; D047495:PPAR gamma / Q000235:genetics / Q000378:metabolism; D011518:Proto-Oncogene Proteins / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015534:Trans-Activators / Q000235:genetics / Q000378:metabolism; D000099232:Proto-Oncogene Protein Spi-1
D016207:Cytokines; D051766:Early Growth Response Protein 1; D018799:Intercellular Adhesion Molecule-1; D009584:Nitrogen; D047495:PPAR gamma; D011518:Proto-Oncogene Proteins; D015534:Trans-Activators; D000099232:Proto-Oncogene Protein Spi-1; C497325:EGR1 protein, human