Detection of trace amounts of abamectin used as an antiparasitic agent in fallow deer tissues.
A sensitive and reliable method for the determination of trace amounts of abamectin in muscles, kidneys and fat tissue of fallow deer is presented. Abamectin was extracted from the tissues with acetonitrile and the extract was cleaned up on a C8 solid-phase extraction cartridge. Abamectin residue was derivatised with trifluoroacetic acid anhydride and 1-methylimidazole, and determined using reversed-phase high-performance liquid chromatography under isocratic conditions and fluorescence detection. The recoveries of the method were high and consistent, ranging from 78% to 90%. The limit of detection of the method was below 1 microg/kg when analysing muscle, kidney and fat tissue. Matrix-matched calibration was used in order to obtain accurate values and to avoid matrix interference.
Zele
Diana
D
Institute for Breeding and Health Care of Wild Animals, Fishes and Bees, Veterinary Faculty, University of Ljubljana, Gerbiceva 60, 1000 Ljubljana, Slovenia. diana.zele@vf.uni-lj.si
Kobal
Silvestra
S
Vengust
Gorazd
G
Bidovec
Andrej
A
Vengust
Anton
A
Tavcar-Kalcher
Gabrijela
G
D000273:Adipose Tissue / Q000737:chemistry*; D000818:Animals; D000977:Antiparasitic Agents / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000662:veterinary; D003670:Deer / Q000378:metabolism*; D007559:Ivermectin / Q000031:analogs & derivatives* / Q000032:analysis; D007668:Kidney / Q000737:chemistry*; D018482:Muscle, Skeletal / Q000737:chemistry*
D000977:Antiparasitic Agents; C048324:abamectin; D007559:Ivermectin