The effect of downhill running on Notch signaling in regenerating skeletal muscle.
Notch signaling expression in regenerating muscle following injurious downhill running (DHR) was characterized in male C57BL/J6 mice (3 mo). Hindlimb muscles were harvested from control mice or at 24, 48, 72, 96 or 120 h post-DHR. Muscle injury was observed at 96 h (3.3-fold) and 120 h (3.7-fold) post-DHR (P < 0.01) and elevated MCadherin expression at 72 h (2.7-fold), 96 h (2.4-fold) post-DHR (P < 0.05) and 120 h (3.3-fold) post-DHR (P < 0.01). Desmin increased at 72 h (2.2-fold), 96 h (3-fold) and 120 h (1.8-fold) post-DHR (P < 0.05). Delta1 +/MCadherin + cells increased approximately 2-fold at 72, 96 (P < 0.01) and 120 h post-DHR (P < 0.05). Isolated muscle-associated cells increased Delta1 (2.6-fold) (P < 0.05) and Notch1 (fourfold) (P < 0.01) expression at 120 h post-DHR. The results of this novel study indicate that DHR up-regulates Notch components within myoblasts and regenerating muscle.
Tsivitse
Susan K
SK
Exercise Physiology Laboratory, Department of Kinesiology, University North Carolina, Charlotte, Charlotte, NC 28223, USA. susantsivitse@uncc.edu
Peters
Michael G
MG
Stoy
Angel L
AL
Mundy
Jeffrey A
JA
Bowen
Robert S
RS
D000818:Animals; D015820:Cadherins / Q000378:metabolism; D002478:Cells, Cultured; D003893:Desmin / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018482:Muscle, Skeletal / Q000293:injuries / Q000378:metabolism / Q000502:physiology*; D010805:Physical Conditioning, Animal / Q000502:physiology; D051880:Receptors, Notch / Q000378:metabolism / Q000502:physiology*; D012038:Regeneration / Q000502:physiology*; D012420:Running / Q000502:physiology*; D015398:Signal Transduction / Q000502:physiology; D013997:Time Factors
D015820:Cadherins; D003893:Desmin; D051880:Receptors, Notch