[Chemical synthesis of lactococcin B and functional evaluation of the N-terminal domain using a truncated synthetic analogue].
The lactococcin B (LnB) is a hydrophobic, positively charged bacteriocin, produced by Lactococcus lactis ssp. cremoris 9B4. It consists of a peptidic chain made up of 47 amino acid residues, and inhibits Lactococcus exclusively. In order to study its biological activity a synthetic lactococcin B (LnBs) was obtained by solid-phase chemical synthesis using a Fmoc strategy. LnBs was shown to be indistinguishable from the natural peptide. In addition, a synthetic (7-47) LnBst analogue was obtained by withdrawal of peptidyl-resin after the 41 cycle of LnBs peptide chain assembly. The synthetic N-terminal truncated (7-47) LnBst analogue was found to be inactive on indicator strains. Our results strongly suggest that the first six N-terminal amino acid residues are involved in the bactericidal activity of LnB.
Lasta
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Laboratoire de Biochimie, CNRS FRE 2738, Faculté de Médecine Nord, Rd Pierre Dramard, 13916 Marseille Cedex 20, France. lasta_samar@yahoo.fr
Fajloun
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Mansuelle
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P
Sabatier
J M
JM
Boudabous
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A
Sampieri
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F
D000595:Amino Acid Sequence / Q000235:genetics; D000596:Amino Acids / Q000235:genetics; D001430:Bacteriocins / Q000138:chemical synthesis* / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid; D005449:Fluorenes; D057927:Hydrophobic and Hydrophilic Interactions; D013294:Lactococcus lactis / Q000737:chemistry / Q000235:genetics / Q000502:physiology; D008969:Molecular Sequence Data; D010455:Peptides / Q000138:chemical synthesis / Q000737:chemistry / Q000235:genetics; D017433:Protein Structure, Secondary / Q000235:genetics; D016415:Sequence Alignment; D020539:Sequence Analysis, Protein; D017386:Sequence Homology, Amino Acid; D052616:Solid Phase Extraction / Q000379:methods
D000596:Amino Acids; D001430:Bacteriocins; D005449:Fluorenes; C016456:N(alpha)-fluorenylmethyloxycarbonylamino acids; D010455:Peptides; C075150:lactococcin B