title: Chemical constituents in roots of Osbeckia opipara.
abstract: OBJECTIVE
  To study the chemical constituents of the roots of Osbeckia opipara.
  
  
  METHODS
  Repeated column chromatography over silica gel, RP-18 and Sephadex LH-20, and preparative thin layer chromatography(PTLC) were used to isolate the compounds, whose structures were determined by spectroscopic methods by direct comparing spectral data with those reported references.
  
  
  RESULTS
  From the MeOH extract of the roots O. opipara, twelve compounds were isolated and identified as follows: lasiodiplodin (1) , de-O-methyllasiodiplodin (2), 2, 3- dihydro-2-hydroxy-2, 4-dimethyl-5-trans-propenylfuran-3-one (3), integracin (4), 5alpha, 8alpha-epidioxy-(22E, 24R)-ergosta-6, 22-dien-3beta-ol (5), 3, 3', 4'-tri-O-methylellagic acid (6), 5-hydroxymethyl furaldehyde (7), vomifolio (8) , betulintic acid (9), 2alpha-hydroxyursolic acid (10), (24R)-stigmast-4-ene-3-one (11), and eugenitin (12).
  
  
  CONCLUSIONS
  Compounds 1-12 were isolated from O. opipara for the first time.
authors.affiliation: Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650204, China. whsh2007@163.com
authors.forename: Hongsheng
authors.initials: H
authors.lastname: Wang
authors.forename: Yuehu
authors.initials: Y
authors.lastname: Wang
authors.forename: Yana
authors.initials: Y
authors.lastname: Shi
authors.forename: Xingyu
authors.initials: X
authors.lastname: Li
authors.forename: Chunlin
authors.initials: C
authors.lastname: Long
mesh_terms: D002783:Cholestenones / Q000737:chemistry / Q000302:isolation & purification*; D005285:Fermentation; D048690:Fruiting Bodies, Fungal / Q000737:chemistry; D035322:Melastomataceae / Q000737:chemistry*; D015394:Molecular Structure; D010936:Plant Extracts / Q000737:chemistry; D018517:Plant Roots / Q000737:chemistry*; D013057:Spectrum Analysis; D014315:Triterpenes / Q000737:chemistry / Q000302:isolation & purification*
chemical_list: C412810:2-hydroxyursolic acid; D002783:Cholestenones; D010936:Plant Extracts; D014315:Triterpenes