Berberine inhibits Staphylococcus epidermidis adhesion and biofilm formation on the surface of titanium alloy.
Biofilm formed by Staphylococcus epidermidis (S. epidermidis) is a common cause of periprosthetic infection. Recently, we have discovered that berberine is bacteriostatic for S. epidermidis. The purpose of the present study was to examine the effect of berberine on S. epidermidis adhesion and biofilm formation on the surface of titanium alloy, which is a popular material for orthopedic joint prostheses. Three strains of S. epidermidis (ATCC 35984, ATCC 12228, and SE 243) were used for in vitro experiment. Direct colony counting showed that berberine significantly inhibited S. epidermidis adhesion on the titanium alloy disk in 2 h at the concentration of 45 microg/mL. When examined with crystal violet staining, confocal laser scanning microscopy, and scanning electron microscopy, we found that higher concentrations (>30 microg/mL) of berberine effectively prevented the formation of S. epidermidis biofilm on the surface of the titanium disk in 24 h. These findings suggest that berberine is a potential agent for the treatment of periprosthetic infection.
Wang
Xiaoqing
X
Department of Orthopaedic Surgery, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
Qiu
Shijing
S
Yao
Xiao
X
Tang
Tingting
T
Dai
Kerong
K
Zhu
Zhen'an
Z
D000497:Alloys; D001422:Bacterial Adhesion / Q000187:drug effects*; D001599:Berberine / Q000494:pharmacology*; D018441:Biofilms / Q000187:drug effects / Q000254:growth & development; D018613:Microscopy, Confocal; D008855:Microscopy, Electron, Scanning; D019736:Prostheses and Implants / Q000009:adverse effects; D013203:Staphylococcal Infections / Q000517:prevention & control; D013212:Staphylococcus epidermidis / Q000187:drug effects*; D014025:Titanium
D000497:Alloys; D001599:Berberine; D014025:Titanium