[Effect of small interfering RNA targeting CXCR4 on breast cancer angiogenesis].
OBJECTIVE
To study the effects of a small interfering RNA targeting CXCR-4 (shRNA-CXCR4) on angiogenesis of human breast cancer cells.


METHODS
The expression of CXCR4 mRNA and protein in 3 breast cancer cell lines with CXCR-4 silencing mediated by shRNA-CXCR4 was detected by RT-PCR and Western blotting, respectively. The morphological changes of human umbilical vein endothelial cells (HUVECs) were observed in co-culture with human breast cancer cells after CXCR4 gene silencing.


RESULTS
CXCR4 mRNA and protein expressions decreased significantly in MCF-7, MDA-MB-231 and MDA-MB-435s breast cancer cells after the gene silencing (P<0.05). Gene silencing with shRNA-CXCR4 in human breast cancer cells significantly inhibited the ability of HUVECs to form tubular structures in the co-culture (P<0.05).


CONCLUSIONS
Gene silencing by shRNA-CXCR4 can obviously lower the angiogenesis-inducing ability of human breast cancer cells.
Li
Hong-yuan
HY
Department of General Surgery, First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China. hongy_li@hotmail.com
Ren
Guo-sheng
GS
Tan
Jin-xiang
JX
D001943:Breast Neoplasms / Q000098:blood supply / Q000235:genetics*; D045744:Cell Line, Tumor; D018920:Coculture Techniques; D042783:Endothelial Cells / Q000166:cytology*; D005260:Female; D006801:Humans; D009389:Neovascularization, Pathologic / Q000235:genetics*; D034622:RNA Interference; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D034741:RNA, Small Interfering / Q000235:genetics*; D019718:Receptors, CXCR4 / Q000235:genetics* / Q000378:metabolism; D014471:Umbilical Veins / Q000166:cytology
C527276:CXCR4 protein, human; D012333:RNA, Messenger; D034741:RNA, Small Interfering; D019718:Receptors, CXCR4