Basophils contribute to T(H)2-IgE responses in vivo via IL-4 production and presentation of peptide-MHC class II complexes to CD4+ T cells.
Basophils express major histocompatibility complex class II, CD80 and CD86 and produce interleukin 4 (IL-4) in various conditions. Here we show that when incubated with IL-3 and antigen or complexes of antigen and immunoglobulin E (IgE), basophils internalized, processed and presented antigen as complexes of peptide and major histocompatibility complex class II and produced IL-4. Intravenous administration of ovalbumin-pulsed basophils into naive mice 'preferentially' induced the development of naive ovalbumin-specific CD4+ T cells into T helper type 2 (T(H)2) cells. Mice immunized in this way, when challenged by intravenous administration of ovalbumin, promptly produced ovalbumin-specific IgG1 and IgE. Finally, intravenous administration of IgE complexes rapidly induced T(H)2 cells only in the presence of endogenous basophils, which suggests that basophils are potent antigen-presenting cells that 'preferentially' augment T(H)2-IgE responses by capturing IgE complex.
Yoshimoto
Tomohiro
T
Department of Immunology and Medical Zoology, Hyogo College of Medicine, Nishinomiya, Hyogo, Japan.
Yasuda
Koubun
K
Tanaka
Hidehisa
H
Nakahira
Masakiyo
M
Imai
Yasutomo
Y
Fujimori
Yoshihiro
Y
Nakanishi
Kenji
K
D019264:Adoptive Transfer; D000818:Animals; D000938:Antigen-Presenting Cells / Q000166:cytology / Q000276:immunology* / Q000378:metabolism; D001491:Basophils / Q000276:immunology* / Q000378:metabolism / Q000637:transplantation; D015496:CD4-Positive T-Lymphocytes / Q000166:cytology / Q000276:immunology* / Q000378:metabolism; D005434:Flow Cytometry; D000949:Histocompatibility Antigens Class II / Q000737:chemistry / Q000276:immunology*; D007073:Immunoglobulin E / Q000276:immunology*; D015847:Interleukin-4 / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D018345:Mice, Knockout; D010047:Ovalbumin / Q000276:immunology; D010455:Peptides / Q000737:chemistry / Q000276:immunology; D013154:Spleen / Q000166:cytology / Q000276:immunology; D013321:Strongyloides / Q000276:immunology; D013322:Strongyloidiasis / Q000276:immunology / Q000469:parasitology; D018418:Th2 Cells / Q000276:immunology
D000949:Histocompatibility Antigens Class II; D010455:Peptides; D015847:Interleukin-4; D007073:Immunoglobulin E; D010047:Ovalbumin