title: Efficient replication bypass of size-expanded DNA base pairs in bacterial cells.
abstract: Supersize me! Size-expanded DNA bases (xDNA) are able to encode natural DNA sequences in replication. In vitro experiments with a DNA polymerase show nucleotide incorporation opposite the xDNA bases with correct pairing. In vivo experiments using E. coli show that two xDNA bases (xA and xC, see picture) encode the correct replication partners.
authors.affiliation: Department of Chemistry, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
authors.forename: James C
authors.initials: JC
authors.lastname: Delaney
authors.forename: Jianmin
authors.initials: J
authors.lastname: Gao
authors.forename: Haibo
authors.initials: H
authors.lastname: Liu
authors.forename: Nidhi
authors.initials: N
authors.lastname: Shrivastav
authors.forename: John M
authors.initials: JM
authors.lastname: Essigmann
authors.forename: Eric T
authors.initials: ET
authors.lastname: Kool
mesh_terms: D020029:Base Pairing; D004247:DNA / Q000737:chemistry*; D004261:DNA Replication; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D007700:Kinetics; D009711:Nucleotides / Q000737:chemistry*
chemical_list: D009711:Nucleotides; D004247:DNA; D004259:DNA-Directed DNA Polymerase