Identification of the human mature B cell miRNome.
The full set of microRNAs (miRNAs) in the human genome is not known. Because presently known miRNAs have been identified by virtue of their abundant expression in a few cell types, many tissue-specific miRNAs remain unrevealed. To understand the role of miRNAs in B cell function and lymphomagenesis, we generated short-RNA libraries from normal human B cells at different stages of development (naive, germinal center, memory) and from a Burkitt lymphoma cell line. A combination of cloning and computational analysis identified 178 miRNAs (miRNome) expressed in normal and/or transformed B cell libraries. Most notably, the B cell miRNome included 75 miRNAs which to our knowledge have not been previously reported and of which 66 have been validated by RNA blot and/or RT-PCR analyses. Numerous miRNAs were expressed in a stage- or transformation-specific fashion in B cells, suggesting specific functional or pathologic roles. These results provide a resource for studying the role of miRNAs in B cell development, immune function, and lymphomagenesis.
Basso
Katia
K
Institute of Cancer Genetics and Herbert Irving Comprehensive Cancer Center, Columbia University, New York, NY 10032, USA.
Sumazin
Pavel
P
Morozov
Pavel
P
Schneider
Christof
C
Maute
Roy L
RL
Kitagawa
Yukiko
Y
Mandelbaum
Jonathan
J
Haddad
Joseph
J
Chen
Chang-Zheng
CZ
Califano
Andrea
A
Dalla-Favera
Riccardo
R
D000818:Animals; D016175:B-Lymphocyte Subsets / Q000276:immunology*; D045744:Cell Line, Tumor; D004285:Dogs; D019143:Evolution, Molecular; D005786:Gene Expression Regulation; D000882:Haplorhini; D006801:Humans; D051379:Mice; D035683:MicroRNAs / Q000032:analysis / Q000235:genetics / Q000502:physiology*; D020411:Oligonucleotide Array Sequence Analysis; D014066:Palatine Tonsil / Q000378:metabolism; D051381:Rats
D035683:MicroRNAs