title: [Investigating the risk of two model virus in the laboratory].
abstract: OBJECTIVE
  To study the survival time of recombination rival in environment and inactivation ability of different disinfectant and ultraviolet radiation against virus.
  
  
  METHODS
  NC membranes absorbed the recombinant adenovirus (rADV) or herpes simplex virus (rHSV) with green fluorescence protein (GFP) were laid, or immersed in various concentration of different disinfectants such as ethanol, sodium hypochlorite, lysol and geramine and then taked out them every 15 min, or exposed under ultraviolet radiation, then the NC membranes were adsorbed 1 h in cell, 37 degrees C 5% CO2 48 h. The results were observed under the fluorescence microscope.
  
  
  RESULTS
  (1) the average survival time of rHSV under environment is less than 60 min, rADV is almost up to 2 h. (2) The infection ability of rHSV and rADV was inactived 15 min by both ethanol (100%, 70% and 50%) and sodium hypochlorite (5%, 2.5% and 1.25%). (3) Two virus can be killed by 0.1% bromogeramine. (4) Both 5% and 2.5% lysol, but rADV can not lost the infection on Vero Cell until 75 min by 1.25% Lysol. (5) The rHSV was inactivated under ultraviolet radiation, but rADV was not.
  
  
  CONCLUSIONS
  The survival time of is different from both envelope rival and the no-envelope viral under nature environment and the inactivate ability of disinfectant also is different between two model virus; Disinfectant should be choose according to virus type.
authors.affiliation: Medical School, Anhui University of Science & Technology, Huainan 232001, China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Li
authors.forename: Guo-yong
authors.initials: GY
authors.lastname: Mei
authors.forename: Hui-ying
authors.initials: HY
authors.lastname: Jiang
authors.forename: Gui-rong
authors.initials: GR
authors.lastname: Wang
authors.forename: Chan
authors.initials: C
authors.lastname: Tian
authors.forename: Lu
authors.initials: L
authors.lastname: Han
authors.forename: Wei-fang
authors.initials: WF
authors.lastname: Han
authors.forename: Qiang
authors.initials: Q
authors.lastname: Wei
authors.forename: Yong-yun
authors.initials: YY
authors.lastname: Zhou
authors.forename: Gui-zhen
authors.initials: GZ
authors.lastname: Wu
authors.forename: Ke-xia
authors.initials: KX
authors.lastname: Wang
authors.forename: Jun
authors.initials: J
authors.lastname: Han
mesh_terms: D000256:Adenoviridae / Q000187:drug effects / Q000528:radiation effects; D004202:Disinfectants / Q000633:toxicity*; D004203:Disinfection / Q000379:methods; D012306:Risk; D018139:Simplexvirus / Q000187:drug effects / Q000528:radiation effects; D012973:Sodium Hypochlorite / Q000633:toxicity; D013242:Sterilization / Q000379:methods; D014466:Ultraviolet Rays; D014777:Virus Diseases; D038301:Virus Inactivation; D018406:Virus Physiological Phenomena / Q000187:drug effects / Q000528:radiation effects; D014780:Viruses / Q000187:drug effects* / Q000528:radiation effects*
chemical_list: D004202:Disinfectants; D012973:Sodium Hypochlorite