Proposal of a low-cost protocol for colorimetric semi-quantification of secretory phospholipase by Candida albicans grown in planktonic and biofilm phases.
Biofilms are aggregates of microorganisms living in multilayered structures inside polymeric matrices onto surfaces. These biofilms may subvert the physiological properties of adjacent tissues causing morphofunctional failure. Many studies have shown that the expression of virulence attributes is maximized when microbes form such communities. This study evaluated the differential phospholipasic activity of Candida albicans SC5314 grown in planktonic phase and in biofilm. We propose two distinct protocols for the colorimetric evaluation of phosphatidylcholine hydrolysis in neutral and acidic conditions. The results showed that both protocols are suitable for the proposed intention and that 72 h-old planktonic cultures of C. albicans SC5314 secrete higher quantities of neutral (6.42-fold) and acidic (3.85-fold) phospholipases than biofilms.
Taniguchi
Lisa
L
Laboratory of Stomatology, Faculty of Dentistry, The Pontifical Catholic University of Paraná, Rua Imaculada Conceição 1155, Curitiba, Brazil.
de Fátima Faria
Berenice
B
Rosa
Rosimeire Takaki
RT
de Paula E Carvalho
Alessandra
A
Gursky
Lauren Christine
LC
Elifio-Esposito
Selene Lobo
SL
Parahitiyawa
Nipuna
N
Samaranayake
Lakshman Perera
LP
Rosa
Edvaldo Antonio Ribeiro
EA
D018441:Biofilms; D002176:Candida albicans / Q000201:enzymology* / Q000502:physiology; D003124:Colorimetry / Q000191:economics* / Q000379:methods*; D005656:Fungal Proteins / Q000378:metabolism*; D010713:Phosphatidylcholines / Q000378:metabolism; D010740:Phospholipases / Q000378:metabolism*
D005656:Fungal Proteins; D010713:Phosphatidylcholines; D010740:Phospholipases