title: Velocity, processivity, and individual steps of single myosin V molecules in live cells.
abstract: We report the tracking of single myosin V molecules in their natural environment, the cell. Myosin V molecules, labeled with quantum dots, are introduced into the cytoplasm of living HeLa cells and their motion is recorded at the single molecule level with high spatial and temporal resolution. We perform an intracellular measurement of key parameters of this molecular transporter: velocity, processivity, step size, and dwell time. Our experiments bridge the gap between in vitro single molecule assays and the indirect measurements of the motor features deduced from the tracking of organelles in live cells.
authors.affiliation: Institut Curie, Centre de Recherche, Laboratoire Physico-Chimie Curie, Centre National de la Recherche Scientifique, UMR 168, Université Pierre et Marie Curie-Paris, Paris, France.
authors.forename: Paolo
authors.initials: P
authors.lastname: Pierobon
authors.forename: Sarra
authors.initials: S
authors.lastname: Achouri
authors.forename: Sébastien
authors.initials: S
authors.lastname: Courty
authors.forename: Alexander R
authors.initials: AR
authors.lastname: Dunn
authors.forename: James A
authors.initials: JA
authors.lastname: Spudich
authors.forename: Maxime
authors.initials: M
authors.lastname: Dahan
authors.forename: Giovanni
authors.initials: G
authors.lastname: Cappello
mesh_terms: D000818:Animals; D019921:Biotinylation; D002147:Calmodulin / Q000378:metabolism; D002470:Cell Survival; D003593:Cytoplasm / Q000378:metabolism; D005456:Fluorescent Dyes / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D042541:Intracellular Space / Q000378:metabolism; D009068:Movement; D024701:Myosin Type V / Q000032:analysis / Q000378:metabolism*; D021381:Protein Transport; D045663:Quantum Dots; D011817:Rabbits; D012666:Semiconductors; D013194:Staining and Labeling; D019809:Streptavidin / Q000378:metabolism; D013997:Time Factors
chemical_list: D002147:Calmodulin; D005456:Fluorescent Dyes; D019809:Streptavidin; D024701:Myosin Type V