Expression patterns of cell adhesion molecules in mice's lung after administration of meso-2,3-dimercaptosuccinic acid-coated maghemite nanoparticles.
We studied the expression pattern of cell adhesion molecules associated to transendothelial migration of leukocytes in different lung's vascular compartments after administration of a magnetic fluid sample containing maghemite nanoparticles surface-coated with meso-2,3-dimercaptosuccinic acid. The analyses were conducted in mice 4 and 12 h after endovenous administration of the magnetic fluid in control mice. Firstly, the migratory activity of leukocytes after magnetic fluid surface-coated with meso-2,3-dimercaptosuccinic acid administration was confirmed using broncho-alveolar lavage and light microscopy. Then, the expression of cell adhesion molecules in the lung's vascular compartments was investigated by immunofluorescence microscopy of frozen sections, using antibodies against L-selectin, P-selectin, E-selectin, macrophage antigen-1, and leukocyte function associated antigen-1. L- and P-selectin showed similar pattern of expression in the pulmonary vasculature in animals treated with magnetic fluid and in the control group. In contrast, macrophage antigen-1 and leukocyte function associated antigen-1 were found in capillary only in animals treated with magnetic fluid surface-coated with meso-2,3-dimercaptosuccinic acid administration. In addition, after magnetic fluid administration E-selectin was found in post-capillary sites. Our findings demonstrated that magnetic fluid surface-coated with meso-2,3-dimercaptosuccinic acid administration exhibits modulation effects on expression patterns of E-selectin, macrophage antigen-1, and leukocyte function associated antigen-1 in the lung's vascular compartments. These findings are very important in a strategy to reduce the potential toxicity of magnetic fluid surface-coated with meso-2,3-dimercaptosuccinic acid administration for medical applications.
Valois
Caroline R A
CR
University of Brasília, Institute of Biological Sciences, Department of Genetics and Morphology, Brasília DF 70910-900, Brazil.
Nunes
Eloiza S
ES
Jaeger
Ruy G
RG
Lima
Emília C D
EC
Morais
Paulo C
PC
Azevedo
Ricardo B
RB
D000818:Animals; D015815:Cell Adhesion Molecules / Q000378:metabolism*; D002465:Cell Movement; D005290:Ferric Compounds / Q000138:chemical synthesis / Q000737:chemistry*; D007962:Leukocytes / Q000166:cytology / Q000187:drug effects*; D008168:Lung / Q000098:blood supply / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure; D008280:Magnetics; D008297:Male; D051379:Mice; D053758:Nanoparticles / Q000008:administration & dosage* / Q000737:chemistry / Q000648:ultrastructure; D004113:Succimer / Q000008:administration & dosage / Q000494:pharmacology*
D015815:Cell Adhesion Molecules; D005290:Ferric Compounds; C000499:ferric oxide; D004113:Succimer