title: Confocal microscopy reveals Myzitiras and Vthela morphotypes as new signatures of malignancy progression.
abstract: G3S1 cells are a new line derived from EM-G3 breast cancer cells by chronic nutritional stress and treatments with 12-O-tetradecanoylphorbol-13-acetate. These cells are capable of growing in standard medium. G3S1 cells exhibited elevated invasiveness in Matrigel invasion chambers as compared with parental EM-G3 cells. Elevated invasiveness of G3S1 cells was accompanied by higher incidence of myzitiras morphotype (sucker-like) and newly observed vthela morphotype (leech-like) both inducible in Hanks' Balanced Salt Solution test. Time-lapse phase contrast microscopy showed a capacity of G3S1 cells to form lobopodial protrusions already 20 min after seeding on gelatin. These protrusions could make contact with the dish and possibly produce the vthela shape. The possible relationship of mysitiras and vthela morphotypes to an increase in malignant potential marked by enhanced invasiveness was thus indicated.
authors.affiliation: Institute of Molecular Genetics AS, Prague, Czech Republic.
authors.forename: Pavel
authors.initials: P
authors.lastname: Veselý
authors.forename: Daniel
authors.initials: D
authors.lastname: Rösel
authors.forename: Daniela
authors.initials: D
authors.lastname: Panková
authors.forename: Ondrej
authors.initials: O
authors.lastname: Tolde
authors.forename: Chris
authors.initials: C
authors.lastname: Blase
authors.forename: Eva
authors.initials: E
authors.lastname: Matousková
authors.forename: Petr
authors.initials: P
authors.lastname: Folk
authors.forename: Jan
authors.initials: J
authors.lastname: Brábek
authors.forename: Juergen
authors.initials: J
authors.lastname: Bereiter-Hahn
mesh_terms: D000818:Animals; D001943:Breast Neoplasms / Q000473:pathology*; D045744:Cell Line, Tumor; D005260:Female; D006801:Humans; D051379:Mice; D018613:Microscopy, Confocal; D008858:Microscopy, Phase-Contrast; D018715:Microscopy, Video