title: Crystal structure and metal binding properties of the lipoprotein MtsA, responsible for iron transport in Streptococcus pyogenes.
abstract: An ability to acquire iron is essential for the viability and growth of almost all organisms and in pathogenic bacteria is strongly correlated with virulence. The cell surface lipoprotein MtsA, a component of the MtsABC transporter of Streptococcus pyogenes, acts as the primary receptor for inorganic iron by this significant human pathogen. Iron is bound as Fe(2+), with the participation of bicarbonate. The crystal structure of MtsA has been determined and refined at 1.8 A resolution (R = 0.167, and R(free) = 0.194). MtsA has the classic bacterial metal binding receptor (MBR) fold, with the Fe(2+) ion bound to the side chains of His68, His140, Glu206, and Asp281, at a totally enclosed site between the two domains of the protein. The absence of bicarbonate from the binding site suggests that it is displaced during the final stages of metal binding. Both the fold and metal binding site are most similar to those of the manganese receptors PsaA and MntC, consistent with the similar coordination requirements of Fe(2+) and Mn(2+). Binding studies confirm a 10-fold preference for Fe(2+) over Mn(2+), although both may be carried in vivo. Mutational analysis of the binding site shows that His140 is critical for a fully functional binding site but that Glu206 is dispensable. The crystal structure explains the distinct roles of these ligands and also reveals potential secondary binding sites that may explain the binding behavior of MtsA for metal ions other than Fe(2+).
authors.affiliation: Institute of Life and Health Engineering and National Research Center of Genetic Medicine, Jinan University, Guangzhou 510632, PR China.
authors.forename: Xuesong
authors.initials: X
authors.lastname: Sun
authors.forename: Heather M
authors.initials: HM
authors.lastname: Baker
authors.forename: Ruiguang
authors.initials: R
authors.lastname: Ge
authors.forename: Hongzhe
authors.initials: H
authors.lastname: Sun
authors.forename: Qing-Yu
authors.initials: QY
authors.lastname: He
authors.forename: Edward N
authors.initials: EN
authors.lastname: Baker
mesh_terms: D019943:Amino Acid Substitution; D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D001665:Binding Sites; D001692:Biological Transport; D018360:Crystallography, X-Ray; D006860:Hydrogen Bonding; D007501:Iron / Q000378:metabolism*; D007700:Kinetics; D008074:Lipoproteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D008345:Manganese / Q000737:chemistry; D008670:Metals / Q000378:metabolism*; D008958:Models, Molecular; D011485:Protein Binding / Q000235:genetics; D011487:Protein Conformation; D017433:Protein Structure, Secondary; D011994:Recombinant Proteins / Q000737:chemistry; D013297:Streptococcus pyogenes / Q000737:chemistry*
chemical_list: D001426:Bacterial Proteins; D008074:Lipoproteins; D008670:Metals; C542731:MtsA lipoprotein, Streptococcus pyogenes; D011994:Recombinant Proteins; D008345:Manganese; D007501:Iron