Simple and efficient enzymatic transglycosylation of stevioside by beta-cyclodextrin glucanotransferase from Bacillus firmus.
Stevioside was subjected to 1,4-intermolecular transglycosylation using beta-cyclodextrin glucanotransferase (beta-CGtase) produced from an alkalophilic strain of Bacillus firmus. The reaction was carried out by traditional, ultrasound-assisted and microwave-assisted techniques. Reaction under microwave conditions was faster and was completed in 1 min yielding two 1,4 transglycosylated products, 4'-O-alpha-D: -glycosyl stevioside (I) and 4''-O-alpha-D: -maltosyl stevioside (II) in 66% and 24%, respectively. The optimum transglycosylation occurred by using stevioside (1.24 mmol), beta-CD (1.76 mmol) and beta-CGtase (2 U/g) under microwave assisted reaction (MAR) in 5 ml sodium phosphate buffer (pH 7) at 50 degrees C and 80 W power. MAR is therefore potentially a useful and economical method for faster transglycosylation of stevioside.
Jaitak
V
V
Natural Plant Products Division, Institute of Himalayan Bioresource Technology (CSIR), Post Bag#06, Palampur 176061, H.P, India.
Kaul
V Kumar
VK
Bandna
Kumar
N
N
Singh
B
B
Savergave
L S
LS
Jogdand
V V
VV
Nene
S
S
D001407:Bacillus / Q000201:enzymology*; D001426:Bacterial Proteins / Q000378:metabolism*; D002021:Buffers; D045786:Diterpenes, Kaurane / Q000378:metabolism*; D005960:Glucosides / Q000378:metabolism*; D005964:Glucosyltransferases / Q000378:metabolism*; D006031:Glycosylation; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008872:Microwaves; D013696:Temperature; D047392:beta-Cyclodextrins / Q000378:metabolism*
D001426:Bacterial Proteins; D002021:Buffers; D045786:Diterpenes, Kaurane; D005960:Glucosides; D047392:beta-Cyclodextrins; C012043:stevioside; D005964:Glucosyltransferases; C019963:cyclomaltodextrin glucanotransferase; C031215:betadex