title: Quorum-sensing signal production by Agrobacterium vitis strains and their tumor-inducing and tartrate-catabolic plasmids.
abstract: Agrobacterium vitis strains, their tumor-inducing (pTi) and tartrate utilization (pTr) plasmid transconjugants and grapevine tumors were analyzed for the presence of N-acyl-homoserine lactones (AHLs). All wild-type A. vitis strains produced long-chain signals. PCR analysis of the A. vitis long-chain AHL synthase gene, avsI, showed the predicted amplicon. Agrobacterium tumefaciens UBAPF2 harboring various A. vitis pTi plasmids produced N-(3-oxo-octanoyl)-l-homoserine lactone encoded also by pTis of A. tumefaciens. UBAPF2 transconjugants carrying pTrs except for pTrTm4 and pTrAB3, also produced an AHL. UBAPF2 transconjugants carrying pTrAT6, pTrAB4 and pTrRr4 or pTiNi1 produced two additional AHLs not observed in the corresponding wild-type strains. We also provide evidence for in situ production of AHLs in grapevine crown gall tumors of greenhouse and field origin.
authors.affiliation: Department of Biological Sciences, Rochester Institute of Technology, Rochester, NY, USA.
authors.forename: Nathanial
authors.initials: N
authors.lastname: Lowe
authors.forename: Han Ming
authors.initials: HM
authors.lastname: Gan
authors.forename: Vandana
authors.initials: V
authors.lastname: Chakravartty
authors.forename: Russell
authors.initials: R
authors.lastname: Scott
authors.forename: Erno
authors.initials: E
authors.lastname: Szegedi
authors.forename: Thomas J
authors.initials: TJ
authors.lastname: Burr
authors.forename: Michael A
authors.initials: MA
authors.lastname: Savka
mesh_terms: D054742:Acyl-Butyrolactones / Q000032:analysis*; D005798:Genes, Bacterial; D010957:Plasmids; D053038:Quorum Sensing; D012231:Rhizobium / Q000737:chemistry / Q000502:physiology*; D027843:Vitis / Q000382:microbiology
chemical_list: D054742:Acyl-Butyrolactones