Cadmium induction of lipid peroxidation and effects on root tip cells and antioxidant enzyme activities in Vicia faba L.
The effects of different concentrations (1-50 microM) of Cd on root growth, cell division and nucleoli in root tip cells, protective enzyme activities and lipid peroxidation in Vicia faba were investigated in order to better understand the processes of Cd-induced senescence. The results indicated that lower concentration of Cd (1 microM) had no obviously influence on the root growth during 24-48 h treatment, but higher concentrations (5-50 microM) inhibited significantly after 48 and 72 h. The mitotic index decreased with increasing of Cd concentration and duration of treatment except for the group exposed to 1 microM Cd. Cd induced c-mitosis, chromosome bridges, chromosome stickiness and lagging chromosomes. The rate of aberrant dividing cells increased with prolonging duration of treatment and increasing of Cd concentration. On nucleolus, some particulates containing the argyrophilic proteins were extruded from the nucleus into the cytoplasm in the cells stressed by Cd and some were scattered in the nucleus. After the treatment with Cd (10 microM Cd, 48 h), the nucleolus did not disaggregate normally and still remain its characteristic structure during metaphase and the particles of similar silver-stained materials were localized on chromosomes. In leaves, Catalase (CAT) activity declined but Peroxidase (POD) activity increased with increasing of the duration of treatment. In roots, CAT activity increased with increasing of the duration of treatment, POD activity increased during early days and then declined. Superoxide dismutase (SOD) activity showed an upward trend with increasing of the duration of treatment after 3 and 6 days, then declined both in leaves and roots (9 days). SOD and POD had highest activities at 50 microM Cd in leaves. CAT activity was lowest at 50 microM Cd. Malondialdehyde (MDA) content increased with the increasing of Cd concentrations and duration of treatment in leaves. In roots, MDA content showed an upward trend with increasing of the duration of treatment at early time and then declined.
Zhang
Shanshan
S
Department of Biology, College of Chemistry and Life Sciences, Tianjin Normal University, 300387, Tianjin, People's Republic of China.
Zhang
Huimin
H
Qin
Rong
R
Jiang
Wusheng
W
Liu
Donghua
D
D019256:Cadmium Chloride / Q000633:toxicity*; D002869:Chromosome Aberrations / Q000139:chemically induced; D018744:DNA, Plant / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D016166:Free Radical Scavengers / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects*; D018519:Meristem / Q000166:cytology / Q000187:drug effects* / Q000502:physiology; D008938:Mitosis / Q000187:drug effects; D008940:Mitotic Index; D009697:Nucleolus Organizer Region / Q000187:drug effects; D018384:Oxidative Stress / Q000187:drug effects*; D010544:Peroxidases / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism; D031307:Vicia faba / Q000166:cytology / Q000187:drug effects* / Q000502:physiology; D014874:Water Pollutants, Chemical / Q000633:toxicity*
D018744:DNA, Plant; D016166:Free Radical Scavengers; D014874:Water Pollutants, Chemical; D010544:Peroxidases; D013482:Superoxide Dismutase; D019256:Cadmium Chloride