Cytotoxicity of withanolides isolated from Acnistus arborescens.
A new withanolide identified by spectroscopic analysis as 12beta-acetoxy-4-deoxy-5,6-deoxy-Delta(5)-withanolide D and Withanolide D, were isolated from the leaves of Acnistus arborescens. Cytotoxic activity of these two compounds against human tumor cell lines HT-29, MCF-7, MKN-45, HEp-2, HeLa, U-937 and two human normal fibroblast cultures, Fib04 and Fib05, were assessed. Withanolide D presented in vitro cytotoxic activity against tumor cell lines at the low micromolar range (LC(50):1.0 to 1.69 microM) and showed a slightly lower activity against Fib04, suggesting moderated selectivity among tumoral and normal cells. No cytotoxic effect was observed for 12beta-acetoxy-4-deoxy-5,6-deoxy-Delta(5)-withanolide D.
Cordero
Claudia P
CP
Cancer Pharmacogenetics Research Group, Departamento de Farmacia, Facultad de Ciencias, Universidad Nacional de Colombia, Carrera 30 No 45-03, Edificio 450, Ciudad Universitaria, Bogotá D.C., Colombia.
Morantes
Sandra J
SJ
Páez
Alexandra
A
Rincón
Javier
J
Aristizábal
Fabio A
FA
D055516:2S Albumins, Plant; D000972:Antineoplastic Agents, Phytogenic / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use*; D002460:Cell Line; D045744:Cell Line, Tumor; D004875:Ergosterol / Q000031:analogs & derivatives* / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use; D005347:Fibroblasts; D006367:HeLa Cells; D006801:Humans; D009369:Neoplasms / Q000188:drug therapy*; D008517:Phytotherapy; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use*; D019657:Solanaceae / Q000737:chemistry*; D054358:Withanolides / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use*
C544624:12-acetoxy-4-deoxy-5,6-deoxy-delta5-withanolide D; D055516:2S Albumins, Plant; D000972:Antineoplastic Agents, Phytogenic; D010936:Plant Extracts; D054358:Withanolides; C010376:withanolide D; D004875:Ergosterol