title: A novel tyrosine-phosphorylated protein inhibiting the growth of Streptomyces cells.
abstract: Very few of the tyrosine-phosphorylated proteins in Streptomyces have been identified. Here, we identify a tyrosine-phosphorylated protein from Streptomyces coelicolor A3(2), designated as SCO5717. The protein possesses Walker motifs and a tyrosine cluster at the C-terminus. When sco5717 harboring its own promoter was introduced into the S. coelicolor cell, the growth was inhibited. An sco5717-disrupted mutant formed aerial mycelium earlier than the wild-type strain, suggesting that SCO5717 controls the cell growth of S. coelicolor. Although the recombinant SCO5717 showed an ATPase activity, it lacked self-phosphorylation ability, suggesting that SCO5717 is a novel tyrosine-phosphorylated protein, which is distinguishable from bacterial protein tyrosine kinases known so far.
authors.affiliation: Department of Molecular Microbiology and Biotechnology, Graduate School of Biomedical Sciences, Hiroshima University, Kasumi 1-2-3, Minami-ku, Hiroshima 734-8551, Japan.
authors.forename: Takanori
authors.initials: T
authors.lastname: Kumagai
authors.forename: Haruhi
authors.initials: H
authors.lastname: Kihara
authors.forename: Wakako
authors.initials: W
authors.lastname: Watanabe
authors.forename: Masafumi
authors.initials: M
authors.lastname: Noda
authors.forename: Yasuyuki
authors.initials: Y
authors.lastname: Matoba
authors.forename: Masanori
authors.initials: M
authors.lastname: Sugiyama
mesh_terms: D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D008969:Molecular Sequence Data; D010766:Phosphorylation; D011505:Protein-Tyrosine Kinases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D048372:Streptomyces coelicolor / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development*; D014443:Tyrosine / Q000378:metabolism*
chemical_list: D011994:Recombinant Proteins; D014443:Tyrosine; D011505:Protein-Tyrosine Kinases