Molecular isolation of the M gene suggests that a conserved-residue conversion induces the formation of bisexual flowers in cucumber plants.
Sex determination in plants involves a variety of mechanisms. Here, we report the map-based cloning and characterization of the unisexual-flower-controlling gene M. M was identified as a previously characterized putative 1-aminocyclopropane-1-carboxylic acid synthase gene, while the m allele that mutated at a conserved site (Gly33Cys) lost activity in the original enzymatically active allele.
Li
Zheng
Z
School of Agriculture and Biology, Shanghai Jiaotong University, 200240, China.
Huang
Sanwen
S
Liu
Shiqiang
S
Pan
Junsong
J
Zhang
Zhonghua
Z
Tao
Qianyi
Q
Shi
Qiuxiang
Q
Jia
Zhiqi
Z
Zhang
Weiwei
W
Chen
Huiming
H
Si
Longting
L
Zhu
Lihuang
L
Cai
Run
R
D000483:Alleles; D001483:Base Sequence; D003001:Cloning, Molecular / Q000379:methods; D017124:Conserved Sequence / Q000235:genetics; D018553:Cucumis sativus / Q000235:genetics*; D035264:Flowers / Q000235:genetics*; D017343:Genes, Plant / Q000235:genetics*; D008969:Molecular Sequence Data; D009154:Mutation; D010940:Plant Proteins / Q000235:genetics
D010940:Plant Proteins