title: Automated soft agar assay for the high-throughput screening of anticancer compounds.
abstract: We have established an automated soft agar colony formation assay that can be used as a potent tool in experimental tumor therapy studies as well as anticancer compound screening. It allows the direct and simultaneous comparison of the effects of a high number of anticancer compounds on the anchorage-independent growth of a variety of tumor cell lines. By making use of a commercially available automated pipetting system, the user gets results of excellent quality within 1 week and does not need special cell culture practice.
authors.affiliation: Institute for Food Toxicology and Analytical Chemistry, University of Veterinary Medicine Hannover, Bischofsholer Damm 15, 30173 Hannover, Germany. rene.thierbach@tiho-hannover.de
authors.forename: René
authors.initials: R
authors.lastname: Thierbach
authors.forename: Pablo
authors.initials: P
authors.lastname: Steinberg
mesh_terms: D000362:Agar; D045744:Cell Line, Tumor; D004354:Drug Screening Assays, Antitumor / Q000379:methods*; D006801:Humans
chemical_list: D000362:Agar