title: Electrochemistry of cytochromes p450: analysis of current-voltage characteristics of electrodes with immobilized cytochromes p450 for the screening of substrates and inhibitors.
abstract: In the current study, an approach to elucidating the substrate specificity of cytochromes P450 based on the analysis of current-voltage characteristics of voltammograms and amperograms is proposed. Data on the electrochemical behavior of bioelectrodes with immobilized cytochromes P450 2B4, 1A2, 3A4, 11A1 (P450scc), and 51b1 (Mycobacterium tuberculosis sterol 14alpha-demethylase or CYP51 MT) in the presence of typical substrates and inhibitors for these hemoprotein forms are reported. Immobilization of the enzymes was accomplished by using graphite screen-printed electrodes modified with gold nanoparticles and with the synthetic membrane-like compound didodecyldimethylammonium bromide. The method of electro-analysis can be applied to the search of potential substrates and inhibitors of cytochromes P450 and to creation of multichannel electrochemical plates (chips, panels) with immobilized cytochromes P450.
authors.affiliation: Institute of Biomedical Chemistry, Russian Academy of Medical Sciences, Moscow, 119121, Russia. viktoria.shumyantseva@ibmc.msk.ru
authors.forename: V V
authors.initials: VV
authors.lastname: Shumyantseva
authors.forename: T V
authors.initials: TV
authors.lastname: Bulko
authors.forename: G P
authors.initials: GP
authors.lastname: Kuznetsova
authors.forename: N F
authors.initials: NF
authors.lastname: Samenkova
authors.forename: A I
authors.initials: AI
authors.lastname: Archakov
mesh_terms: D000818:Animals; D065607:Cytochrome P-450 Enzyme Inhibitors; D003577:Cytochrome P-450 Enzyme System / Q000737:chemistry* / Q000378:metabolism; D004353:Drug Evaluation, Preclinical / Q000379:methods*; D004563:Electrochemistry / Q000379:methods*; D004566:Electrodes; D004791:Enzyme Inhibitors / Q000737:chemistry*; D004800:Enzymes, Immobilized / Q000037:antagonists & inhibitors* / Q000737:chemistry* / Q000378:metabolism; D009169:Mycobacterium tuberculosis / Q000737:chemistry / Q000201:enzymology; D011817:Rabbits; D013379:Substrate Specificity
chemical_list: D065607:Cytochrome P-450 Enzyme Inhibitors; D004791:Enzyme Inhibitors; D004800:Enzymes, Immobilized; D003577:Cytochrome P-450 Enzyme System