Longitudinal changes of defensive and offensive factors in focal cerebral ischemia-reperfusion in rats.
The cerebral ischemia-reperfusion injury remains a major medical problem due to the lack of effective treatment. The mechanism of brain injury is still unknown. The defensive and offensive factors, such as platelet-derived growth factor-BB (PDGF-BB), 5-lipoxygenase (5-LO), aquaporin-4 (AQP-4) and insulin-like growth factor-1 (IGF-1) may play important roles. So far, only individual factors were reported. What are the relationships among them in brain ischemia-reperfusion injury remains obscure. The present study is to investigate simultaneously the expression of PDGF-BB, 5-LO, AQP-4 and IGF-1 in middle cerebral artery occlusion/reperfusion (MCAO/R) in rats. We found that 5-LO and IGF-1 reached the peak level at 24h after reperfusion, AQP-4 at 72 h and PDGF-BB at 7 days. With these results we inferred that both defensive factors, such as PDGF-BB, AQP-4 and IGF-1, and offensive factor, like 5-LO, play some roles in the ischemia-reperfusion injury.
Hu
Ming
M
Department of Neurology, The Second Hospital of Hebei Medical University, Shijiazhuang, Hebei 050000, China.
Zhang
Xiangjian
X
Liu
Wei
W
Cui
Haiying
H
Di
Nan
N
D000704:Analysis of Variance; D000818:Animals; D051401:Aquaporin 4 / Q000378:metabolism*; D001094:Arachidonate 5-Lipoxygenase / Q000378:metabolism*; D000077214:Becaplermin; D001921:Brain / Q000378:metabolism / Q000473:pathology; D007150:Immunohistochemistry; D020244:Infarction, Middle Cerebral Artery / Q000378:metabolism* / Q000473:pathology; D007334:Insulin-Like Growth Factor I / Q000378:metabolism*; D008297:Male; D009043:Motor Activity; D009483:Neuropsychological Tests; D010982:Platelet-Derived Growth Factor / Q000378:metabolism*; D020574:Proto-Oncogene Proteins c-sis; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000378:metabolism* / Q000473:pathology; D013997:Time Factors; D014867:Water / Q000378:metabolism
C496032:Aqp4 protein, rat; D051401:Aquaporin 4; D010982:Platelet-Derived Growth Factor; D020574:Proto-Oncogene Proteins c-sis; D014867:Water; D000077214:Becaplermin; D007334:Insulin-Like Growth Factor I; D001094:Arachidonate 5-Lipoxygenase