Biodegradation of the sulfonylurea herbicide chlorimuron-ethyl by the strain Pseudomonas sp. LW3.
The chlorimuron-ethyl-degrading bacterium LW3 was isolated from contaminated soil and identified by 16S rRNA gene sequencing as Pseudomonas sp. When chlorimuron-ethyl was provided as the sole nitrogen source, the degradation efficiency in liquid medium was about 81.0% after 7 days of inoculation with strain LW3. The effects of chlorimuron-ethyl concentration and temperature on biodegradation were examined. Two metabolites of degradation were analyzed by LC/MS. Based on the identified products, strain LW3 seemed to be able to degrade chlorimuron-ethyl by cleavage of the sulfonylurea bridge. The inoculation of strain LW3 to chlorimuron-ethyl-treated soil resulted in a higher degradation rate than in uninoculated soil, regardless of the soil being sterilized or nonsterilized. This microbial culture has great potential for the bioremediation of soil contaminated with chlorimuron-ethyl.
Ma
Ji-Ping
JP
Key Laboratory for Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, College of Life Sciences, Nanjing Agricultural University, Nanjing, China.
Wang
Zhe
Z
Lu
Peng
P
Wang
Hui-jie
HJ
Waseem Ali
Shinawar
S
Li
Shun-Peng
SP
Huang
Xing
X
D001711:Biotransformation; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D006540:Herbicides / Q000378:metabolism*; D008969:Molecular Sequence Data; D010802:Phylogeny; D011549:Pseudomonas / Q000302:isolation & purification / Q000378:metabolism*; D011743:Pyrimidines / Q000378:metabolism*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D013453:Sulfonylurea Compounds / Q000378:metabolism*
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D006540:Herbicides; D011743:Pyrimidines; D012336:RNA, Ribosomal, 16S; D013453:Sulfonylurea Compounds; C112343:chlorimuron ethyl