title: Methylation analysis of BRCA1, RASSF1, GSTP1 and EPHB2 promoters in prostate biopsies according to different degrees of malignancy.
abstract: Prostate cancer is the most common cancer among men and the second leading cause of cancer-related deaths in the United States. CpG island methylation causes gene silencing and could be decisive in prostate carcinogenesis and progression. Its role was investigated at multiple gene sites during prostate carcinogenesis. Methylation-specific polymerase chain reaction (MS-PCR) was used to analyze 4 interest gene promoter status in 12 patients with adenocarcinoma, 7 patients with prostate intraepithelial neoplasia, 3 patients with peritumor tissues and 15 healthy patients, so a total of 37 prostate biopsy samples constituted the cohort of the study. Despite the biopsy histology, the results have confirmed that BRCA1, RASSF1, GSTP1 and EPHB2 promoter methylation was found in each sample, except two.
authors.affiliation: Département d'Oncogénétique du Centre Jean Perrin, 63011 Clermont-Ferrand Cedex 01, France.
authors.forename: Nadège
authors.initials: N
authors.lastname: Rabiau
authors.forename: Mohamed Oury
authors.initials: MO
authors.lastname: Thiam
authors.forename: Samir
authors.initials: S
authors.lastname: Satih
authors.forename: Laurent
authors.initials: L
authors.lastname: Guy
authors.forename: Jean-Louis
authors.initials: JL
authors.lastname: Kemeny
authors.forename: Jean-Paul
authors.initials: JP
authors.lastname: Boiteux
authors.forename: Luc
authors.initials: L
authors.lastname: Fontana
authors.forename: Yves-Jean
authors.initials: YJ
authors.lastname: Bignon
authors.forename: Dominique
authors.initials: D
authors.lastname: Bernard-Gallon
mesh_terms: D001483:Base Sequence; D019175:DNA Methylation; D017931:DNA Primers; D019398:Genes, BRCA1; D051549:Glutathione S-Transferase pi / Q000235:genetics*; D006801:Humans; D008297:Male; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic; D011471:Prostatic Neoplasms / Q000145:classification / Q000235:genetics* / Q000473:pathology; D036183:Receptor, EphB2 / Q000235:genetics*; D025521:Tumor Suppressor Proteins / Q000235:genetics*
chemical_list: D017931:DNA Primers; C411762:RASSF1 protein, human; D025521:Tumor Suppressor Proteins; D051549:Glutathione S-Transferase pi; D036183:Receptor, EphB2