Chemical constituents in roots of Osbeckia opipara.
OBJECTIVE
To study the chemical constituents of the roots of Osbeckia opipara.


METHODS
Repeated column chromatography over silica gel, RP-18 and Sephadex LH-20, and preparative thin layer chromatography(PTLC) were used to isolate the compounds, whose structures were determined by spectroscopic methods by direct comparing spectral data with those reported references.


RESULTS
From the MeOH extract of the roots O. opipara, twelve compounds were isolated and identified as follows: lasiodiplodin (1) , de-O-methyllasiodiplodin (2), 2, 3- dihydro-2-hydroxy-2, 4-dimethyl-5-trans-propenylfuran-3-one (3), integracin (4), 5alpha, 8alpha-epidioxy-(22E, 24R)-ergosta-6, 22-dien-3beta-ol (5), 3, 3', 4'-tri-O-methylellagic acid (6), 5-hydroxymethyl furaldehyde (7), vomifolio (8) , betulintic acid (9), 2alpha-hydroxyursolic acid (10), (24R)-stigmast-4-ene-3-one (11), and eugenitin (12).


CONCLUSIONS
Compounds 1-12 were isolated from O. opipara for the first time.
Wang
Hongsheng
H
Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650204, China. whsh2007@163.com
Wang
Yuehu
Y
Shi
Yana
Y
Li
Xingyu
X
Long
Chunlin
C
D002783:Cholestenones / Q000737:chemistry / Q000302:isolation & purification*; D005285:Fermentation; D048690:Fruiting Bodies, Fungal / Q000737:chemistry; D035322:Melastomataceae / Q000737:chemistry*; D015394:Molecular Structure; D010936:Plant Extracts / Q000737:chemistry; D018517:Plant Roots / Q000737:chemistry*; D013057:Spectrum Analysis; D014315:Triterpenes / Q000737:chemistry / Q000302:isolation & purification*
C412810:2-hydroxyursolic acid; D002783:Cholestenones; D010936:Plant Extracts; D014315:Triterpenes