title: [RP-HPLC determination of tiliroside in Daphne genkwa].
abstract: OBJECTIVE
  To determine the contents of tiliroside [kaempferol 3-O-beta-D-(6 " -p-coumaroyl) glucopyranoside] in the Daphne genkwa.
  
  
  METHODS
  The separation was performed on ZORBAX XDB C18 column (4.6 mm x 250 mm, 5 microm), using acetonitrile and water/acetic acid mixture (1,000/1) (gradient elution: 0-18 min, 26 : 74; 18-33 min, 80 : 20) as mobile phase with the rate of 1.0 mL x min(-1) at 25 degrees C, the wavelength for measurement was 310 nm.
  
  
  RESULTS
  The linearity was in the range of 0.1-5.0 microg (n=6), and the correlation coefficient was 0.9999. The average recovery for compound tiliroside was 101.50% (RSD 0.90%) (n=6).
  
  
  CONCLUSIONS
  This method is accurate, reliable and reproducible to determine tiliroside in the D. genkwa.
authors.affiliation: College of Chemistry of JiLin University, Changchun 130012, China.
authors.forename: Lili
authors.initials: L
authors.lastname: Song
authors.forename: Juan
authors.initials: J
authors.lastname: Lu
authors.forename: Peifang
authors.initials: P
authors.lastname: Yan
authors.forename: Lina
authors.initials: L
authors.lastname: Tan
authors.forename: Xuwen
authors.initials: X
authors.lastname: Li
authors.forename: Yongri
authors.initials: Y
authors.lastname: Jin
mesh_terms: D000097:Acetonitriles / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D032364:Daphne / Q000737:chemistry*; D005419:Flavonoids / Q000302:isolation & purification*; D009822:Oils, Volatile / Q000494:pharmacology; D018547:Plant Stems / Q000737:chemistry
chemical_list: D000097:Acetonitriles; C118943:Daphne repellent; D005419:Flavonoids; D009822:Oils, Volatile; C052083:tiliroside; C032159:acetonitrile