[Comparison of 3 techniques for artificial shrinkage of blastocoelic cavity before vitrification of expanded mouse blastocysts].
OBJECTIVE
To establish an effective pretreatment method for the vitrification of expanded mouse blastocysts by comparing 3 techniques for the artificial shrinkage of the blastocoelic cavity.


METHODS
The blastocoelic cavity was artificially shrunk by micro-needle aspiration, pipetting or laser drilling prior to the vitrification of the expanded blastocysts. The rates of survival and hatching achieved with the three techniques were compared with those of the non-shrinkage group.


RESULTS
The rates of survival were 72.9, 72.0 and 94.0%, and those of hatching were 64.6, 32.0 and 62.0% in the three shrinkage groups, obviously higher than in the nonshrinkage group (40.0 and 16.0%).


CONCLUSIONS
Artificial shrinkage of the blastocoelic cavity was an effective pretreatment technique for the vitrification of expanded mouse blastocysts, especially by micro-needle aspiration and laser drilling.
Zhang
Ning-yuan
NY
Reproductive Medicine Center, Drum Tower Hospital Affiliated to Nanjing University School of Medicine, Nanjing, Jiangsu 210008, China.
Sun
Hai-xiang
HX
Hu
Ya-li
YL
Xu
Zhi-peng
ZP
Chen
Lin-jun
LJ
Zhao
Xia
X
D000818:Animals; D001755:Blastocyst; D015925:Cryopreservation / Q000379:methods*; D046149:Embryo Culture Techniques / Q000379:methods*; D005260:Female; D005307:Fertilization in Vitro / Q000379:methods; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR