Establishment and characterization of the Bombyx mandarina cell line.
A new cell line, designated as NIAS-Boma-529b, was established from the larval fat bodies of Bombyx mandarina (B. mandarina), which is believed to be an ancestor of Bombyx mori (B. mori). This cell line has been cultured for approximately 150 passages during 2years in an IPL-41 medium supplemented with 10% fetal bovine serum at a constant temperature of 26 degrees C. The morphology of this line includes adhesive round and spindle-shaped cells. Random-amplified polymorphic DNA analysis (RAPD) using 7 primers and a statistical analysis based on Nei's genetic distance revealed that this cell line was closely related to B. mori-derived cell lines. An infection study also revealed that this cell line was susceptible to B. mori nucleopolyhedrovirus (BmNPV); however, it had no apparent susceptibility to Autographa californica NPV (AcNPV), which is closely related to BmNPV. Nevertheless, cells infected with AcNPV showed an extensive cytopathic effect (CPE), including a rough cell surface, rounding, nuclear expansion, and cell blebbing. These results suggest that this cell line can be useful to clarify the mechanism of host range determination of BmNPV and AcNPV.
Iwanaga
Masashi
M
Department of Bioproductive Science, Utsunomiya University, Minemachi, Tochigi, Japan. iwanaga@cc.utsunomiya-u.ac.jp
Arai
Rika
R
Shibano
Yuka
Y
Kawasaki
Hideki
H
Imanishi
Shigeo
S
D000818:Animals; D001483:Base Sequence; D012831:Bombyx / Q000166:cytology* / Q000821:virology; D018929:Cell Culture Techniques; D002460:Cell Line; D049109:Cell Proliferation; D003576:Electron Transport Complex IV / Q000737:chemistry / Q000235:genetics; D005216:Fat Body / Q000166:cytology* / Q000821:virology; D019476:Insect Proteins / Q000737:chemistry / Q000235:genetics; D007814:Larva / Q000166:cytology / Q000821:virology; D008969:Molecular Sequence Data; D017924:Nucleopolyhedroviruses / Q000502:physiology; D016133:Polymerase Chain Reaction; D016415:Sequence Alignment
D019476:Insect Proteins; D003576:Electron Transport Complex IV