Different messenger RNA expression for the antimicrobial peptides beta-defensins between Meishan and crossbred pigs.
beta-Defensins are cysteine-rich endogenously produced antimicrobial peptides that play an important role in innate immunity. In this study, the expressions of genes porcine beta-defensins-1(pBD-1), pBD-2 and pBD-3 were determined using real-time PCR for Chinese Meishan pigs and Crossbred (Duroc x Yorkshire x Landrace) pigs of 7 days old in various tissues. The results showed that expressions of pBD-1, 2 and 3 of Meishan pigs in most tissues were higher than those of crossbred pigs and main expression sites for pBD-1 and pBD-3 were tongue and oral mucosa in two varieties of pigs, whereas pBD-2 of crossbred pig was mainly expressed in kidney and liver, and pBD-2 of Meishan pigs mainly in tongue and oral mucosa. The higher expression of pBDs might be the reason of Meishan pigs has higher immunity and disease resistance. The mechanisms of this need a further research.
Chen
Jinyong
J
Institute of Animal Nutrition, Key Laboratory of Animal Disease-Resistance Nutrition of China Ministry of Education, Sichuan Agricultural University, Sichuan, People's Republic China. cjyyjc@tom.com
Qi
Sharina
S
Guo
Rongfu
R
Yu
Bing
B
Chen
Daiwen
D
D000704:Analysis of Variance; D000818:Animals; D001483:Base Sequence; D003433:Crosses, Genetic; D017931:DNA Primers / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics; D007113:Immunity, Innate / Q000235:genetics*; D007668:Kidney / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D008969:Molecular Sequence Data; D009061:Mouth Mucosa / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D013045:Species Specificity; D034421:Sus scrofa / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D014059:Tongue / Q000378:metabolism; D023083:beta-Defensins / Q000235:genetics / Q000378:metabolism*
D017931:DNA Primers; D018076:DNA, Complementary; D012333:RNA, Messenger; D023083:beta-Defensins