Genome-wide gene expression profiling in gamma-irradiated green alga, Pseudokirchneriella subcapitata, by HiCEP.
Transcriptome was analyzed in gamma-irradiated green alga Pseudokirchneriella subcapitata by high coverage gene expression profiling (HiCEP). Approximately 7,800 expressed genes were detected. Expression levels of 623-707 genes were affected at 100-300 Gy. Nucleotide sequences of 41 up-regulated genes were determined. The quantitative reverse transcription polymerase chain reaction validated the up-regulation. Two genes had homology to genes related to ionizing radiation. These results indicate usefulness of HiCEP for screening of stress-responsive genes in species that are ecotoxicologically important but for which genomic sequence information is lacking.
Fuma
Shoichi
S
Environmental Radiation Effects Research Group, National Institute of Radiological Sciences, 4-9-1 Anagawa, Inage-ku, Chiba, 263-8555, Japan. fuma@nirs.go.jp
Nakamori
Taizo
T
Ishii
Nobuyoshi
N
Kubota
Yoshihisa
Y
Yoshida
Satoshi
S
Fujimori
Akira
A
D000460:Chlorophyta / Q000235:genetics* / Q000528:radiation effects*; D030562:Databases, Protein; D005720:Gamma Rays; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant / Q000235:genetics* / Q000528:radiation effects*; D055106:Genome-Wide Association Study; D018749:RNA, Plant / Q000096:biosynthesis / Q000528:radiation effects; D020133:Reverse Transcriptase Polymerase Chain Reaction
D018749:RNA, Plant