Beta-ketoacyl-ACP synthase I/II from Plasmodium falciparum (PfFabB/F)--is it B or F?
Condensing enzymes play an important and decisive role in terms of fatty acid composition of any organism. They can be classified as condensing enzymes involved in initiating the cycle and enzymes involved in elongating the initiated fatty acyl chain. In E. coli, two isoforms for the elongation condensing enzymes (FabB and FabF) exists whereas Plasmodium genome contains only one isoform. By in vitro complementation studies in E. coli CY244 cells, we show that PfFabB/F functions like E. coli FabF as the growth of the mutant cells could be rescued only in the presence of oleic acid. But unlike bacterial enzyme, PfFabB/F does not increase the cis-vaccenic acid content in the mutant cells upon lowering the growth temperature. This study thus highlights the distinct properties of P. falciparum FabF which sets it apart from E. coli and most other enzymes of this family described so far.
Sharma
Shilpi
S
Molecular Biophysics Unit, Indian Institute of Science, Bangalore, India.
Sharma
Shailendra Kumar
SK
Surolia
Namita
N
Surolia
Avadhesha
A
D015099:3-Oxoacyl-(Acyl-Carrier-Protein) Synthase / Q000378:metabolism*; D000818:Animals; D004926:Escherichia coli / Q000201:enzymology; D029968:Escherichia coli Proteins / Q000378:metabolism; D054889:Fatty Acid Synthase, Type II; D007527:Isoenzymes / Q000378:metabolism*; D013058:Mass Spectrometry; D009829:Oleic Acids / Q000378:metabolism; D010963:Plasmodium falciparum / Q000201:enzymology*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D029968:Escherichia coli Proteins; D007527:Isoenzymes; D009829:Oleic Acids; C065593:cis-vaccenic acid; C078182:beta-ketoacyl-acyl carrier protein synthase I; D015099:3-Oxoacyl-(Acyl-Carrier-Protein) Synthase; C490369:FabB protein, E coli; D054889:Fatty Acid Synthase, Type II