title: [Oxidized low-density lipoprotein enhances the expressions of SREBP-2 and HMGCR mRNA in macrophages derived from the monocytes of patients with acute coronary syndrome].
abstract: OBJECTIVE
  To investigate the effect of oxidized low-density lipoprotein (ox-LDL) on the expressions of sterol regulatory element binding protein-2 (SREBP-2) and hydroxymethylglutaryl CoA reductase (HMGCR) in the macrophages derived from monocytes of patients with acute coronary syndrome (ACS).
  
  
  METHODS
  LDL was oxidized by Cu2+ to prepare ox-LDL, and peripheral monocytes were isolated by density gradient centrifugation from patients with ACS diagnosed by coronary arteriography. Macrophages derived from the monocytes after phorbol myristate acetate (PMA) stimulation were treated with ox-LDL at the concentrations of 0, 20, 40, and 100 ng/ml, and the changes in the expressions of SREBP-2 and HMGCR were detected by real-time RT-PCR.
  
  
  RESULTS
  Compared with the control cells, the macrophages treated with ox-LDL showed significantly increased expressions of SREBP-2 and HMGCR mRNA (P<0.05). In cells treated with ox-LDL, the expressions of SREBP-2 and HMGCR mRNA differed significantly with the dose administered (P<0.05).
  
  
  CONCLUSIONS
  Within a defined dose range, ox-LDL can dose-dependently enhance the expressions of SREBP-2 and HMGCR mRNA in macrophages from patients with ACS.
authors.affiliation: Department of Cardiovascular Medicine, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, China. Zhangpeidong120@sina.com
authors.forename: Pei-dong
authors.initials: PD
authors.lastname: Zhang
authors.forename: Ying-feng
authors.initials: YF
authors.lastname: Liu
authors.forename: Yang
authors.initials: Y
authors.lastname: Guo
authors.forename: Fei
authors.initials: F
authors.lastname: Miao
authors.forename: Zhi-guo
authors.initials: ZG
authors.lastname: Yu
authors.forename: Shi-xiang
authors.initials: SX
authors.lastname: Wang
mesh_terms: D054058:Acute Coronary Syndrome / Q000097:blood*; D004305:Dose-Response Relationship, Drug; D006801:Humans; D006903:Hydroxymethylglutaryl CoA Reductases / Q000235:genetics / Q000378:metabolism*; D008077:Lipoproteins, LDL / Q000494:pharmacology*; D008264:Macrophages / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051782:Sterol Regulatory Element Binding Protein 2 / Q000235:genetics / Q000378:metabolism*
chemical_list: D008077:Lipoproteins, LDL; D012333:RNA, Messenger; C497390:SREBF2 protein, human; D051782:Sterol Regulatory Element Binding Protein 2; C099252:oxidized low density lipoprotein; D006903:Hydroxymethylglutaryl CoA Reductases