title: Field assessment of an H5N1 inactivated vaccine in chickens and ducks in Lao PDR.
abstract: Despite the extensive use of poultry vaccines to control the spread of H5N1 influenza in poultry, H5N1 outbreaks continue to occur in domestic birds. Our objective was to determine the duration of the neutralizing antibody response under field conditions after vaccination with a laboratory-tested inactivated reverse genetics-derived H5N3 vaccine. H5N3 hemagglutination inhibition (HI) and virus neutralization (VN) antibodies were observed 40 weeks after vaccination of chickens with two doses and vaccination of ducks with one dose. Cross-clade antibodies to an H5N1 virus (A/chicken/Laos/A0464/07) antigenically distinct from the vaccine strain were detected in ducks after a single vaccination and were sustained for 28 weeks (for 40 weeks when a boost vaccination was given). Our results indicate that this inactivated H5N3 vaccine can produce long-lasting antibodies to homologous and heterologous viruses under field conditions.
authors.affiliation: Department of Infectious Diseases, Division of Virology, St. Jude Children's Research Hospital, 262 Danny Thomas Place, Memphis, TN 38105-3678, USA.
authors.forename: David A
authors.initials: DA
authors.lastname: Boltz
authors.forename: Bounlom
authors.initials: B
authors.lastname: Douangngeun
authors.forename: Settha
authors.initials: S
authors.lastname: Sinthasak
authors.forename: Phouvong
authors.initials: P
authors.lastname: Phommachanh
authors.forename: Phetlamphone
authors.initials: P
authors.lastname: Midouangchanh
authors.forename: David
authors.initials: D
authors.lastname: Walker
authors.forename: Rachael
authors.initials: R
authors.lastname: Keating
authors.forename: Alexey M
authors.initials: AM
authors.lastname: Khalenkov
authors.forename: Mahesh
authors.initials: M
authors.lastname: Kumar
authors.forename: Robert G
authors.initials: RG
authors.lastname: Webster
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D002645:Chickens; D003429:Cross Reactions; D004372:Ducks; D006385:Hemagglutination Inhibition Tests; D053124:Influenza A Virus, H5N1 Subtype / Q000276:immunology*; D007252:Influenza Vaccines / Q000276:immunology*; D005585:Influenza in Birds / Q000517:prevention & control*; D007812:Laos; D009500:Neutralization Tests; D015164:Vaccines, Inactivated / Q000276:immunology
chemical_list: D000914:Antibodies, Viral; D007252:Influenza Vaccines; D015164:Vaccines, Inactivated