Biodegradation of brominated aromatics by cultures and laccase of Trametes versicolor.
2-Bromophenol (1), 4-bromophenol (2), 2,4-dibromophenol (3), 2,6-dibromophenol (4), 2,4,6-tribromophenol (5) and tetrabromobisphenol A (6) (1 mM each) added to growing submerged cultures of Trametes versicolor CCBAS 612 were eliminated by 65-85% from the culture medium within 4d. Extracellular laccase activity in the culture medium was influenced by the type of brominated compound added. Maximum level of laccase (63 U L(-1)) was found in the culture with 2-bromophenol. Tetrabromobisphenol A was degraded by a commercial laccase from Trametes versicolor in absence of any oxidation mediator, hydroxylated dibrominated compounds being detected as soluble reaction products by LC/MS. A significant degradation of brominated phenols by laccase was achieved only in the presence of ABTS structural characterization of major products suggesting reaction between bromophenol and ABTS radicals.
Uhnáková
B
B
Institute of Microbiology, Academy of Sciences, 142 20 Prague, Czech Republic. brona@biomed.cas.cz
Petrícková
A
A
Biedermann
D
D
Homolka
L
L
Vejvoda
V
V
Bednár
P
P
Papousková
B
B
Sulc
M
M
Martínková
L
L
D001673:Biodegradation, Environmental; D006842:Hydrocarbons, Brominated / Q000737:chemistry / Q000378:metabolism*; D042845:Laccase / Q000378:metabolism*; D010636:Phenols / Q000737:chemistry / Q000378:metabolism*; D011075:Polybrominated Biphenyls / Q000378:metabolism; D055454:Trametes / Q000201:enzymology*; D014874:Water Pollutants, Chemical / Q000737:chemistry / Q000378:metabolism*
D006842:Hydrocarbons, Brominated; D010636:Phenols; D011075:Polybrominated Biphenyls; D014874:Water Pollutants, Chemical; C038964:2,6-dibromophenol; C032038:2-bromophenol; D042845:Laccase; C020806:tetrabromobisphenol A; C013930:2,4-dibromophenol; C032037:4-bromophenol; C004554:2,4,6-tribromophenol