title: Spontaneous [Ca2+]i oscillations in G1/S phase-synchronized cells.
abstract: Ca(2+) signaling controls a wide range of cellular functions such as division, fertilization, apoptosis and necrosis. Specifically, calcium signaling is thought to play a crucial role in driving cells through the different stages of the cell-division cycle. In most cells, however, this fact is far from being established. Few studies have examined this question from a different perspective: whether cells exhibit some characteristic cell cycle-dependent intracellular calcium-signaling patterns. This approach is effective in discerning the causal relationship between Ca(2+) signaling and the cell cycle. Through synchronization of the cell cycle, flow cytometry and confocal scanning microscopic intracellular calcium ion concentration ([Ca(2+)](i)) imaging, the present study shows that the G1/S phase transition is uniquely characterized by spontaneous [Ca(2+)](i) oscillations that last for up to 40 min. Most likely, these oscillations emanate from the [Ca(2+)](i) signaling that accompanies DNA replication as the cell prepares for the next division cycle. These temporal signals further affirm the significance of Ca(2+) in the cell cycle.
authors.forename: Afadhali Denis
authors.initials: AD
authors.lastname: Russa
authors.forename: Chihaya
authors.initials: C
authors.lastname: Maesawa
authors.forename: Yoh-ichi
authors.initials: Y
authors.lastname: Satoh
mesh_terms: D000818:Animals; D019556:COS Cells / Q000166:cytology / Q000378:metabolism; D002118:Calcium / Q000378:metabolism*; D020013:Calcium Signaling; D002453:Cell Cycle / Q000187:drug effects; D002522:Chlorocebus aethiops; D004261:DNA Replication; D005434:Flow Cytometry; D016193:G1 Phase / Q000187:drug effects* / Q000502:physiology; D006367:HeLa Cells / Q000166:cytology / Q000378:metabolism; D006801:Humans; D018613:Microscopy, Confocal; D016196:S Phase / Q000187:drug effects* / Q000502:physiology
chemical_list: D002118:Calcium