Product profile of PEN3: the last unexamined oxidosqualene cyclase in Arabidopsis thaliana.
The triterpene product profile is reported for At5g36150 (PEN3), the last unexamined oxidosqualene cyclase in the reference plant Arabidopsis thaliana. PEN3 makes tirucalla-7,24-dien-3beta-ol ( approximately 85%) and several minor products. Also discussed are the unexpectedly facile convergent evolution of another Arabidopsis tirucalladienol synthase (LUP5), mechanistic origins of the 20S configuration, and active-site remodeling necessary to accommodate the 17alpha side chain. This work marks the first completed functional characterization of all triterpene synthases in a higher plant.
Morlacchi
Pietro
P
Department of Chemistry, Rice University, Houston, Texas 77005, USA.
Wilson
William K
WK
Xiong
Quanbo
Q
Bhaduri
Aparna
A
Sttivend
Diana
D
Kolesnikova
Mariya D
MD
Matsuda
Seiichi P T
SP
D018528:ATP-Binding Cassette Transporters / Q000378:metabolism*; D017360:Arabidopsis / Q000201:enzymology*; D029681:Arabidopsis Proteins / Q000378:metabolism*; D003500:Cyclization; D006801:Humans; D019751:Intramolecular Transferases / Q000378:metabolism*; D015394:Molecular Structure; D014315:Triterpenes / Q000138:chemical synthesis* / Q000378:metabolism*
D018528:ATP-Binding Cassette Transporters; D029681:Arabidopsis Proteins; C510424:PDR8 protein, Arabidopsis; D014315:Triterpenes; D019751:Intramolecular Transferases; C021924:lanosterol synthase