Efficient degradation of lignocellulosic plant biomass, without pretreatment, by the thermophilic anaerobe "Anaerocellum thermophilum" DSM 6725.
Very few cultivated microorganisms can degrade lignocellulosic biomass without chemical pretreatment. We show here that "Anaerocellum thermophilum" DSM 6725, an anaerobic bacterium that grows optimally at 75 degrees C, efficiently utilizes various types of untreated plant biomass, as well as crystalline cellulose and xylan. These include hardwoods such as poplar, low-lignin grasses such as napier and Bermuda grasses, and high-lignin grasses such as switchgrass. The organism did not utilize only the soluble fraction of the untreated biomass, since insoluble plant biomass (as well as cellulose and xylan) obtained after washing at 75 degrees C for 18 h also served as a growth substrate. The predominant end products from all growth substrates were hydrogen, acetate, and lactate. Glucose and cellobiose (on crystalline cellulose) and xylose and xylobiose (on xylan) also accumulated in the growth media during growth on the defined substrates but not during growth on the plant biomass. A. thermophilum DSM 6725 grew well on first- and second-spent biomass derived from poplar and switchgrass, where spent biomass is defined as the insoluble growth substrate recovered after the organism has reached late stationary phase. No evidence was found for the direct attachment of A. thermophilum DSM 6725 to the plant biomass. This organism differs from the closely related strain A. thermophilum Z-1320 in its ability to grow on xylose and pectin. Caldicellulosiruptor saccharolyticus DSM 8903 (optimum growth temperature, 70 degrees C), a close relative of A. thermophilum DSM 6725, grew well on switchgrass but not on poplar, indicating a significant difference in the biomass-degrading abilities of these two otherwise very similar organisms.
Yang
Sung-Jae
SJ
Department of Biochemistry and Molecular Biology, University of Georgia, Athens, GA 30602, USA.
Kataeva
Irina
I
Hamilton-Brehm
Scott D
SD
Engle
Nancy L
NL
Tschaplinski
Timothy J
TJ
Doeppke
Crissa
C
Davis
Mark
M
Westpheling
Janet
J
Adams
Michael W W
MW
D019342:Acetic Acid / Q000378:metabolism; D000693:Anaerobiosis; D018533:Biomass; D002482:Cellulose / Q000378:metabolism; D015169:Colony Count, Microbial; D006094:Gram-Positive Bacteria / Q000254:growth & development / Q000378:metabolism*; D006358:Hot Temperature; D006859:Hydrogen / Q000378:metabolism; D019344:Lactic Acid / Q000378:metabolism; D008031:Lignin / Q000378:metabolism*; D010944:Plants / Q000378:metabolism* / Q000382:microbiology*; D014990:Xylans / Q000378:metabolism
D014990:Xylans; C036909:lignocellulose; D019344:Lactic Acid; D006859:Hydrogen; D002482:Cellulose; D008031:Lignin; D019342:Acetic Acid