title: Detrimental effects of cigarette smoke constituents on physiological development of extraocular and intraocular structures.
abstract: No investigation has yet been accomplished to screen the detrimental effects of cigarette smoke condensate (CSC) and total particular matter solution (TPMS) on embryonic development of extraocular and intraocular structures. In this report, chicken embryo assay was utilized to undermine diverse ocular pathologies produced by exposure of CSC and TPM. Extraocular anomalies triggered after exposure of CSC and TPMS include degeneration of optic chiasma, medial rectus muscle, and inflammatory lesions in forebrain. Histological investigations of CSC and TPMS-treated embryos also exposed delayed differentiation of photoreceptor layer, degeneration of retinal ganglion and nerve cell layer. In addition, corneal thickness, deterioration and complete loss of hyaloid vasculature were observed. Extraocular and intraocular regions of TPMS-treated embryos also revealed widespread hemorrhages in the entire cephalic, optic disc, ganglion cell layer and vitreous humor area. The findings of our experiment demonstrate, for the first time, that exposure to CSC and TPMS is hazardous for developing embryos and it has potential detrimental effects on several underlying events of ocular development. Moreover, it was also intriguing that toxicity profile of TMP was much more higher than CSC with more profound detrimental effects on ocular development.
authors.affiliation: Department of Clinical Neurosciences, R3 Stroke Unit, Addenbrookes Hospital, University of Cambridge, Cambridge, CB2 0QQ, UK. se298@cam.ac.uk
authors.forename: Sohail
authors.initials: S
authors.lastname: Ejaz
authors.forename: Muhammad
authors.initials: M
authors.lastname: Adil
authors.forename: Myong Ho
authors.initials: MH
authors.lastname: Oh
authors.forename: Syed Muhammad Muneeb
authors.initials: SM
authors.lastname: Anjum
authors.forename: Muhammad
authors.initials: M
authors.lastname: Ashraf
authors.forename: Chae Woong
authors.initials: CW
authors.lastname: Lim
mesh_terms: D000818:Animals; D001921:Brain / Q000254:growth & development / Q000473:pathology; D002642:Chick Embryo; D005123:Eye / Q000187:drug effects / Q000196:embryology / Q000254:growth & development*; D007249:Inflammation / Q000139:chemically induced / Q000473:pathology; D009897:Optic Chiasm / Q000254:growth & development / Q000473:pathology; D017568:Rectus Abdominis / Q000254:growth & development / Q000473:pathology; D012160:Retina / Q000254:growth & development / Q000473:pathology; D012906:Smoke / Q000009:adverse effects*; D012996:Solutions; D014026:Nicotiana / Q000633:toxicity*
chemical_list: D012906:Smoke; D012996:Solutions