Curcumin attenuates 6-hydroxydopamine-induced cytotoxicity by anti-oxidation and nuclear factor-kappa B modulation in MES23.5 cells.
Oxidative stress has been implicated in the degeneration of dopaminergic neurons in the substantia nigra of Parkinson's disease patients, and several anti-oxidants have been shown to be effective on the treatment of Parkinson's disease. Curcumin has been previously reported to possess radical scavenger, iron chelating, anti-inflammatory properties in different tissues. The aim of present study is to explore the cytoprotection of curcumin against 6-hydroxydopamine (6-OHDA)-induced neuronal death, as well as the underlying mechanisms in MES23.5 cells. Our results showed that 6-OHDA significantly reduced the cell viability of MES23.5 cells. Curcumin protected MES23.5 cells against 6-OHDA neurotoxicity by partially restoring the mitochondrial membrane potential, increasing the level of Cu-Zn superoxide dismutase and suppressing an increase in intracellular reactive oxygen species. Furthermore, curcumin pretreatment significantly inhibited 6-OHDA induced nuclear factor-kappaB translocation. These results suggest that the neuroprotective effects of curcumin are attributed to the antioxidative properties and the modulation of nuclear factor-kappaB translocation.
Wang
Jun
J
National Key Disciplines: Physiology (in incubation), Department of Physiology, Medical College of Qingdao University, No. 308 Ningxia Road, Qingdao, 266071, China.
Du
Xi-Xun
XX
Jiang
Hong
H
Xie
Jun-Xia
JX
D000818:Animals; D000975:Antioxidants / Q000502:physiology* / Q000633:toxicity; D016923:Cell Death / Q000187:drug effects / Q000502:physiology; D002478:Cells, Cultured; D003474:Curcumin / Q000494:pharmacology*; D051379:Mice; D016328:NF-kappa B / Q000378:metabolism* / Q000502:physiology; D018384:Oxidative Stress / Q000187:drug effects / Q000502:physiology; D016627:Oxidopamine / Q000037:antagonists & inhibitors* / Q000633:toxicity*; D021381:Protein Transport / Q000187:drug effects; D017382:Reactive Oxygen Species / Q000378:metabolism
D000975:Antioxidants; D016328:NF-kappa B; D017382:Reactive Oxygen Species; D016627:Oxidopamine; D003474:Curcumin