Recombinant Modified Vaccinia Ankara (MVA) effectively boosts DNA-primed HIV-specific immune responses in humans despite pre-existing vaccinia immunity.
The presence of vector-specific immune responses may hamper the induction of responses to a foreign antigen encoded by the vector. We evaluated the impact of pre-existing immunity to vaccinia virus on the induction of HIV-specific responses after immunization of healthy volunteers with a HIV-1 DNA prime-MVA boost vaccine. Following three priming immunizations with HIV-1 DNA plasmids, the volunteers were boosted with a single injection of recombinant MVA encoding HIV-1 proteins. Pre-existing immunity to vaccinia virus did not reduce the proportion of individuals who responded to HIV-1, but did lower the magnitude of responses. Our results suggest that vaccinia-based vectors can be used to efficiently induce immune responses to vectored HIV-1 antigens, even in individuals with pre-existing immunity to vaccinia virus.
Gudmundsdotter
Lindvi
L
Karolinska Institutet and Swedish Institute for Infectious Disease Control, Stockholm, Sweden. L.gudmundsdotter@smi.se
Nilsson
Charlotta
C
Brave
Andreas
A
Hejdeman
Bo
B
Earl
Patricia
P
Moss
Bernard
B
Robb
Merlin
M
Cox
Josephine
J
Michael
Nelson
N
Marovich
Mary
M
Biberfeld
Gunnel
G
Sandström
Eric
E
Wahren
Britta
B
D016915:AIDS Vaccines / Q000276:immunology*; D000328:Adult; D000367:Age Factors; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood / Q000276:immunology; D002460:Cell Line; D002522:Chlorocebus aethiops; D005260:Female; D015488:HIV Antigens / Q000276:immunology; D015658:HIV Infections / Q000276:immunology / Q000517:prevention & control*; D015497:HIV-1 / Q000276:immunology*; D006801:Humans; D007111:Immunity, Cellular; D007117:Immunization, Secondary; D008297:Male; D008875:Middle Aged; D009500:Neutralization Tests; D019444:Vaccines, DNA / Q000276:immunology; D014614:Vaccines, Synthetic / Q000276:immunology; D014616:Vaccinia virus / Q000276:immunology*; D055815:Young Adult
D016915:AIDS Vaccines; D000914:Antibodies, Viral; D015488:HIV Antigens; D019444:Vaccines, DNA; D014614:Vaccines, Synthetic