Role of heparanase-1 in gastric carcinoma invasion.
OBJECTIVE
The heparan sulfate-degrading endoglycosidase may mediate tumor invasion and metastasis. It is known that heparanase-1 (HPA-1) plays an important role in cleaving heparan sulfate. In this study we investigated its potential role in gastric carcinoma malignant behaviour.


METHODS
To assess the role of HPA-1, we suppressed its expression using small interfering RNA (siRNA). The human heparanase-1 specific siRNA was transfected to the human gastric carcinoma cell line SGC7901. The effect of gene silencing was assessed by Real-Time PCR and Western Blot. The invasion potential of the cells was evaluated using an in vitro cell invasion model system.


RESULTS
The HPA-1 specific siRNA significantly suppressed expression of heparanase-1 in SGC7901 cells. Invasion after RNAi treatment through membranes in a model system was significantly decreased.


CONCLUSIONS
HPA-1 facilitates gastric carcinoma invasion by up-regulating endoglycosidase activity in tumor cells.
Yingying
Xu
X
Department of Surgical Oncology and General Surgery, the First Hospital of China Medical University, Shenyang, P.R. China.
Yong
Zhang
Z
Zhenning
Wang
W
Xue
Zhang
Z
Li
Jiang
J
Yang
Luo
L
Huimian
Xu
X
D045744:Cell Line, Tumor; D055785:Gene Knockdown Techniques; D020868:Gene Silencing; D005966:Glucuronidase / Q000235:genetics / Q000502:physiology*; D006801:Humans; D009361:Neoplasm Invasiveness / Q000235:genetics / Q000503:physiopathology; D034741:RNA, Small Interfering / Q000235:genetics; D013274:Stomach Neoplasms / Q000235:genetics / Q000473:pathology*; D014407:Tumor Cells, Cultured; D000099175:Heparanase
D005966:Glucuronidase; D034741:RNA, Small Interfering; D000099175:Heparanase