title: Pericentrin, a centrosomal protein related to microcephalic primordial dwarfism, is required for olfactory cilia assembly in mice.
abstract: The Drosophila pericentrin-like protein has been shown to be essential for the formation of the sensory cilia of chemosensory and mechanosensory neurons by mutant analysis in flies, while the in vivo function of pericentrin, a well-studied mammalian centrosomal protein related to microcephalic primordial dwarfism, has been unclear. To determine whether pericentrin is required for ciliogenesis in mammals, we generated and analyzed mice with a hypomorphic mutation of Pcnt encoding the mouse pericentrin. Immunofluorescence analysis demonstrated that olfactory cilia of chemosensory neurons in the nasal olfactory epithelium were malformed in the homozygous mutant mice. On the other hand, the assembly of motile and primary cilia of non-neuronal epithelial cells and the formation of sperm flagella were not affected in the Pcnt-mutant mice. The defective assembly of olfactory cilia in the mutant was apparent from birth. The mutant animals displayed reduced olfactory performance in agreement with the compromised assembly of olfactory cilia. Our findings suggest that pericentrin is essential for the assembly of chemosensory cilia of olfactory receptor neurons, but it is not globally required for cilia formation in mammals.
authors.affiliation: Department of Brain Science, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, 2-5-1 Shikatacho, Okayama 700-8558, Japan. miyoshi@cc.okayama-u.ac.jp
authors.forename: Ko
authors.initials: K
authors.lastname: Miyoshi
authors.forename: Kyosuke
authors.initials: K
authors.lastname: Kasahara
authors.forename: Ikuko
authors.initials: I
authors.lastname: Miyazaki
authors.forename: Shoko
authors.initials: S
authors.lastname: Shimizu
authors.forename: Manabu
authors.initials: M
authors.lastname: Taniguchi
authors.forename: Shinsuke
authors.initials: S
authors.lastname: Matsuzaki
authors.forename: Masaya
authors.initials: M
authors.lastname: Tohyama
authors.forename: Masato
authors.initials: M
authors.lastname: Asanuma
mesh_terms: D000818:Animals; D000941:Antigens / Q000235:genetics / Q000378:metabolism*; D018385:Centrosome / Q000378:metabolism*; D002923:Cilia / Q000378:metabolism; D004392:Dwarfism / Q000235:genetics*; D051379:Mice; D008817:Mice, Mutant Strains; D008831:Microcephaly / Q000235:genetics*; D009830:Olfactory Bulb / Q000002:abnormalities*; D014158:Transcription, Genetic
chemical_list: D000941:Antigens; C086178:pericentrin