title: Clean absorption mode NMR data acquisition based on time-proportional phase incrementation.
abstract: Clean absorption mode NMR data acquisition is presented based on mirrored time domain sampling and widely used time-proportional phase incrementation (TPPI) for quadrature detection. The resulting NMR spectra are devoid of dispersive frequency domain peak components. Those peak components exacerbate peak identification and shift peak maxima, and thus impede automated spectral analysis. The new approach is also of unique value for obtaining clean absorption mode reduced-dimensionality projection NMR spectra, which can rapidly provide high-dimensional spectral information for high-throughput NMR structure determination.
authors.affiliation: Department of Chemistry, The State University of New York at Buffalo, Buffalo, NY 14260, USA.
authors.forename: Yibing
authors.initials: Y
authors.lastname: Wu
authors.forename: Arindam
authors.initials: A
authors.lastname: Ghosh
authors.forename: Thomas
authors.initials: T
authors.lastname: Szyperski
mesh_terms: D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods*; D011487:Protein Conformation; D011506:Proteins / Q000737:chemistry* / Q000378:metabolism
chemical_list: D011506:Proteins