title: Enzymatic assay of D-glucuronate using uronate dehydrogenase.
abstract: D-Glucuronate is a key metabolite in the process of detoxification of xenobiotics and in a recently constructed synthetic pathway to produce D-glucaric acid, a "top value-added chemical" from biomass. A simple and specific assay of D-glucuronate would be useful for studying these processes, but existing assays are either time-consuming or nonspecific. Using uronate dehydrogenase cloned from Agrobacterium tumefaciens, we developed an assay for D-glucuronate with a detection limit of 5 microM. This method was shown to be more suitable for a system with many interfering compounds than previous methods and was also applied to assays for myo-inositol oxygenase activity.
authors.affiliation: Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
authors.forename: Tae Seok
authors.initials: TS
authors.lastname: Moon
authors.forename: Sang-Hwal
authors.initials: SH
authors.lastname: Yoon
authors.forename: Mary-Jane
authors.initials: MJ
authors.lastname: Tsang Mui Ching
authors.forename: Amanda M
authors.initials: AM
authors.lastname: Lanza
authors.forename: Kristala L Jones
authors.initials: KL
authors.lastname: Prather
mesh_terms: D000445:Aldehyde Oxidoreductases / Q000378:metabolism*; D004926:Escherichia coli / Q000201:enzymology; D005965:Glucuronates / Q000032:analysis* / Q000378:metabolism
chemical_list: D005965:Glucuronates; C023280:uronate dehydrogenase; D000445:Aldehyde Oxidoreductases