Isolation of stem cells from adult rat kidneys.
The kidney has an inherent ability for recovery and regeneration following acute damage. However, there has been much contention as to the source of regenerating renal cells. The aim of this study was to isolate and characterize these cells. Normal rat kidneys were minced and cells were isolated with collagenase I and were cultured in an expansion medium. Adherent cells were isolated and expanded for more than 120 days in vitro. These cells had the potential of trans-lineage differentiation into neural cells, adipocytes and osteocytes. These cells also expressed Nucleostemin, Cyclin D1, Notch1 and Survivin which are commonly expressed in stem cells. The results of the current work show that the adult kidney contains a population of multipotent stem cells.
Gheisari
Yousof
Y
Stem Cell Technology Company, Tehran, Iran.
Soleimani
Masoud
M
Zeinali
Sirous
S
Arefian
Ehsan
E
Atashi
Amir
A
Zarif
Mahin Nikougoftar
MN
D000818:Animals; D002352:Carrier Proteins / Q000378:metabolism; D002454:Cell Differentiation / Q000502:physiology; D002469:Cell Separation / Q000379:methods*; D002478:Cells, Cultured; D019938:Cyclin D1 / Q000378:metabolism; D005260:Female; D019204:GTP-Binding Proteins; D007668:Kidney / Q000166:cytology* / Q000502:physiology; D008869:Microtubule-Associated Proteins / Q000378:metabolism; D039902:Multipotent Stem Cells / Q000166:cytology* / Q000378:metabolism; D009687:Nuclear Proteins / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D051881:Receptor, Notch1 / Q000378:metabolism; D012038:Regeneration; D000077022:Survivin; D001613:beta 2-Microglobulin / Q000378:metabolism
C516989:Birc5 protein, rat; D002352:Carrier Proteins; C470310:Gnl3 protein, rat; D008869:Microtubule-Associated Proteins; C497763:Notch1 protein, rat; D009687:Nuclear Proteins; D051881:Receptor, Notch1; D000077022:Survivin; D001613:beta 2-Microglobulin; D019938:Cyclin D1; D019204:GTP-Binding Proteins