Silver nanoparticles inhibit VEGF induced cell proliferation and migration in bovine retinal endothelial cells.
Angiogenesis, the growth of new blood vessels from pre-existing vasculature is of physiological and pathological importance. We have investigated the anti-angiogenic potential of silver nanoparticles, produced by Bacillus licheniformis. Bovine retinal endothelial cells (BRECs) were treated with the different concentrations of silver nanoparticles for 24 h in the presence and absence of vascular endothelial growth factor (VEGF), where 500 nM (IC50) of silver nanoparticle concentration, was able to block the proliferation and migration of BRECs. The cells showed a clear enhancement in caspase-3 activity and formation of DNA ladders, evidence of induction of apoptosis. Here we report for the first time that silver nanoparticles inhibit cell survival via PI3K/Akt dependent pathway in Bovine retinal endothelial cells.
Kalishwaralal
Kalimuthu
K
Department of Biotechnology, Division of Molecular and Cellular Biology, Kalasalingam University, Anand Nagar, Krishnankoil 626190, Tamil Nadu, India.
Banumathi
Elayappan
E
Ram Kumar Pandian
SureshBabu
S
Deepak
Venkataraman
V
Muniyandi
Jeyaraj
J
Eom
Soo Hyun
SH
Gurunathan
Sangiliyandi
S
D020533:Angiogenesis Inhibitors / Q000633:toxicity; D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D001407:Bacillus / Q000737:chemistry; D015153:Blotting, Western; D053148:Caspase 3 / Q000378:metabolism; D002417:Cattle; D002465:Cell Movement / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D053938:DNA Fragmentation / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D042783:Endothelial Cells / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D020128:Inhibitory Concentration 50; D053768:Metal Nanoparticles / Q000737:chemistry / Q000633:toxicity*; D018919:Neovascularization, Physiologic / Q000187:drug effects; D010766:Phosphorylation / Q000187:drug effects; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D012160:Retina / Q000166:cytology; D012834:Silver / Q000737:chemistry; D042461:Vascular Endothelial Growth Factor A / Q000494:pharmacology*
D020533:Angiogenesis Inhibitors; D042461:Vascular Endothelial Growth Factor A; D012834:Silver; D051057:Proto-Oncogene Proteins c-akt; D053148:Caspase 3