Hepatoprotective effect of electrolyzed reduced water against carbon tetrachloride-induced liver damage in mice.
The study investigated the protective effect of electrolyzed reduced water (ERW) against carbon tetrachloride (CCl(4))-induced liver damage. Male ICR mice were randomly divided into control, CCl(4), CCl(4)+silymarin, and CCl(4)+ERW groups. CCl(4)-induced liver lesions include leukocytes infiltration, hepatocyte necrosis, ballooning degeneration, mitosis, calcification, fibrosis and an increase of serum alanine aminotransferase (ALT), and aminotransferase (AST) activity. In addition, CCl(4) also significantly decreased the activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px). By contrast, ERW or silymarin supplement significantly ameliorated the CCl(4)-induced liver lesions, lowered the serum levels of hepatic enzyme markers (ALT and AST) and increased the activities of SOD, catalase, and GSH-Px in liver. Therefore, the results of this study show that ERW can be proposed to protect the liver against CCl(4)-induced oxidative damage in mice, and the hepatoprotective effect might be correlated with its antioxidant and free radical scavenging effect.
Tsai
Chia-Fang
CF
Institute of Medicine, College of Medicine, Chung Shan Medical University, No. 110, Sec. 1, Jianguo N. Rd., Taichung 402, Taiwan.
Hsu
Yu-Wen
YW
Chen
Wen-Kang
WK
Chang
Wen-Huei
WH
Yen
Cheng-Chieh
CC
Ho
Yung-Chyuan
YC
Lu
Fung-Jou
FJ
D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D001652:Bile Ducts / Q000473:pathology; D001835:Body Weight / Q000187:drug effects; D002252:Carbon Tetrachloride Poisoning / Q000201:enzymology / Q000473:pathology* / Q000517:prevention & control*; D056486:Chemical and Drug Induced Liver Injury / Q000517:prevention & control*; D002784:Cholesterol / Q000097:blood; D004572:Electrolysis; D008099:Liver / Q000201:enzymology / Q000473:pathology*; D008111:Liver Function Tests; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D020556:Neutrophil Infiltration / Q000187:drug effects; D009929:Organ Size / Q000187:drug effects; D020011:Protective Agents / Q000627:therapeutic use; D012838:Silymarin / Q000627:therapeutic use; D014280:Triglycerides / Q000097:blood; D014867:Water / Q000737:chemistry*
D000975:Antioxidants; D020011:Protective Agents; D012838:Silymarin; D014280:Triglycerides; D014867:Water; D002784:Cholesterol