The dynamic side of the Hofmeister effect: a single-molecule nanopore study of specific complex formation.
Beyond measurements of equilibria: An alpha-hemolysin nanopore is used as a single-molecule sensor to follow the effects of different salts on the complexation reaction of gamma-cyclodextrin and adamantane carboxylate (see picture). The kinetics underlying the dynamic equilibrium are studied to reveal qualitatively different dynamic actions of various cosolute salts.
Gurnev
Philip A
PA
Laboratory of Physical and Structural Biology, Program in Physical Biology, NICHD, National Institutes of Health, 9000 Rockville Pike, Bldg. 9, Bethesda, MD 20892, USA.
Harries
Daniel
D
Parsegian
V Adrian
VA
Bezrukov
Sergey M
SM
D000218:Adamantane / Q000737:chemistry; D006460:Hemolysin Proteins / Q000737:chemistry*; D057927:Hydrophobic and Hydrophilic Interactions; D007700:Kinetics; D016062:Porosity; D013816:Thermodynamics; D047408:gamma-Cyclodextrins / Q000737:chemistry
D006460:Hemolysin Proteins; D047408:gamma-Cyclodextrins; C023792:gamma-cyclodextrin; D000218:Adamantane