title: Detection of breast micro-metastases in axillary lymph nodes by infrared micro-spectral imaging.
abstract: We report the ability of infrared micro-spectral imaging, coupled with completely unsupervised methods of multivariate statistical analysis, to accurately reproduce the histological architecture of axillary lymph nodes and detect metastatic breast cancer cells. The acquisition of spectral data from tissue embedded in paraffin provided spectra free of dispersive artefacts that may be observed for infrared microscopic measurements using a 'reflection/absorption' methodology. As a consequence, superior tissue classification and identification of cellular abnormality unattainable for deparaffinised tissue was achieved.
authors.affiliation: Laboratory for Spectral Diagnosis (LSpD), Department of Chemistry and Chemical Biology, Northeastern University, 360 Huntington Avenue, Boston, USA. benjamin_bird@hotmail.com
authors.forename: Benjamin
authors.initials: B
authors.lastname: Bird
authors.forename: Kristi
authors.initials: K
authors.lastname: Bedrossian
authors.forename: Nora
authors.initials: N
authors.lastname: Laver
authors.forename: Milos
authors.initials: M
authors.lastname: Miljković
authors.forename: Melissa J
authors.initials: MJ
authors.lastname: Romeo
authors.forename: Max
authors.initials: M
authors.lastname: Diem
mesh_terms: D001943:Breast Neoplasms / Q000378:metabolism / Q000473:pathology*; D006801:Humans; D008207:Lymphatic Metastasis / Q000175:diagnosis* / Q000473:pathology; D008853:Microscopy; D015591:Microspectrophotometry; D013055:Spectrophotometry, Infrared; D013194:Staining and Labeling