20K-GH and its use in detecting GH abuse.
Following the successful production of recombinant 20K-GH, several studies investigating the physiology of this GH isoform have been undertaken. In this report, we review studies of its biological effect, measurement and secretion. To use serum 20K-GH level in detecting GH abuse, new method has been established and serum 22K-GH, 20K-GH were measured in normal subjects and athletes, and no abnormal results were found among athletes. Another study confirmed that serum 22K-GH increased remarkably and 20K-GH decreased following the exogenous administration of 22K-GH. The duration was relatively short, approximately 24-36h in our and other studies. The increase of the ratio, 22K-GH/20K-GH was the most suitable indicator of GH abuse. Studies supported by the WADA were undertaken in collaboration with an Australian Group. A new approach for the GH isoform assay by beads assay platform is being developed. It is concluded that the direct measurement of 20K-GH is a valid scientific approach, for detecting GH abuse, although the duration of the positive results is short. Our method will be useful in combination with the marker method, an out-of-competition test or test for target cases. Furthermore, its application for the doping test passport is considered to be a possible future strategy.
Irie
Minoru
M
The Foundation for Growth Science and Toho University, Tokyo, Japan. iriemc@concerto.plala.or.jp
Ueki
Makoto
M
Kishikawa
Yoshifumi
Y
Nishii
Manabu
M
Kawahara
Takashi
T
D000293:Adolescent; D000328:Adult; D000595:Amino Acid Sequence; D056352:Athletes; D002624:Chemistry, Clinical / Q000379:methods; D004300:Doping in Sports; D005260:Female; D019382:Human Growth Hormone / Q000032:analysis / Q000627:therapeutic use*; D006801:Humans; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D010446:Peptide Fragments; D020033:Protein Isoforms; D015813:Substance Abuse Detection / Q000379:methods*
D010446:Peptide Fragments; D020033:Protein Isoforms; C404802:human growth hormone 22K; C039519:somatotropin 20K; D019382:Human Growth Hormone