title: Transcriptional profiling of cellulase and expansin-related genes in a hypercellulolytic Trichoderma reesei.
abstract: Expression kinetics of six cellulase and four expansin-related genes were studied in the hypercellulolytic Trichoderma reesei CL847 mutant in response to Solka Floc cellulose and soluble inducers. Real-time PCR showed a parallel increase of transcript levels for the cellulase genes cbh1/cel7a, egl1/cel7b, egl4/cel61a, the beta-glucosidase genes bgl1/cel3a, bgl2/cel1a, and the swo1 gene, encoding the cell-wall loosening protein swollenin. To evaluate a putative implication of three newly identified expansin/family 45 endoglucanase-like (EEL) proteins in lignocellulose degradation, their expression was also analysed. Only eel2 was found to be transcribed under the present conditions, and showed constitutive expression similar to the endoglucanase encoding cel5b gene.
authors.affiliation: Département de Biotechnologie, IFP, 1 et 4 Avenue de Bois-Préau, 92852 Rueil-Malmaison, France.
authors.forename: Jonathan
authors.initials: J
authors.lastname: Verbeke
authors.forename: Pedro
authors.initials: P
authors.lastname: Coutinho
authors.forename: Hugues
authors.initials: H
authors.lastname: Mathis
authors.forename: Alain
authors.initials: A
authors.lastname: Quenot
authors.forename: Eric
authors.initials: E
authors.lastname: Record
authors.forename: Marcel
authors.initials: M
authors.lastname: Asther
authors.forename: Senta
authors.initials: S
authors.lastname: Heiss-Blanquet
mesh_terms: D002480:Cellulase / Q000096:biosynthesis*; D002482:Cellulose / Q000378:metabolism; D005656:Fungal Proteins / Q000096:biosynthesis*; D020869:Gene Expression Profiling; D015971:Gene Expression Regulation, Enzymologic; D015966:Gene Expression Regulation, Fungal; D005800:Genes, Fungal; D014242:Trichoderma / Q000201:enzymology* / Q000235:genetics* / Q000378:metabolism; D001617:beta-Glucosidase / Q000096:biosynthesis
chemical_list: D005656:Fungal Proteins; D002482:Cellulose; D001617:beta-Glucosidase; D002480:Cellulase