Streptomyces plumbiresistens sp. nov., a lead-resistant actinomycete isolated from lead-polluted soil in north-west China.
The taxonomic position of an actinomycete isolated from a lead-polluted soil in Gansu province, north-west China, was determined by using a polyphasic approach. Chemical and morphological properties of the isolate, designated strain CCNWHX 13-160(T), were similar to those of streptomycetes. Analysis of the almost complete 16S rRNA gene sequence placed strain CCNWHX 13-160(T) in the genus Streptomyces where it formed a distinct phyletic line with recognized Streptomyces species. The strain was most similar to Streptomyces pseudovenezuelae NBRC 12904(T) (98.9 %) and Streptomyces resistomycificus NBRC 12814(T) (98.8 %). Furthermore, DNA-DNA hybridization studies between the novel isolate and these two strains showed relatedness values of 49.7+/-0.8 and 43.2+/-1.1 %, respectively. It is proposed that strain CCNWHX 13-160(T) (=ACCC 41207(T)=HAMBI 2991(T)) be classified as the type strain of a novel species in the genus Streptomyces, Streptomyces plumbiresistens sp. nov. The MIC of Pb(2+) for growth of strain CCNWHX 13-160(T) was 4.0 mM.
Guo
Jun Kang
JK
College of Life Sciences, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Northwest A & F University, Yangling Shaanxi 712100, PR China.
Lin
Yan Bing
YB
Zhao
Mei Ling
ML
Sun
Ran
R
Wang
Ting Ting
TT
Tang
Ming
M
Wei
Ge Hong
GH
D015373:Bacterial Typing Techniques; D001482:Base Composition; D002681:China; D004269:DNA, Bacterial / Q000032:analysis; D004275:DNA, Ribosomal / Q000032:analysis; D024881:Drug Resistance, Bacterial; D005838:Genotype; D007854:Lead / Q000494:pharmacology*; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010641:Phenotype; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000494:pharmacology*; D013302:Streptomyces / Q000145:classification* / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D012989:Soil Pollutants; D007854:Lead