Fe(II) phthalocyanine catalyzed oxidation of dGMP by molecular oxygen.
We show that iron(II)-phthalocyanines are able to catalyze guanosine oxidation by molecular oxygen in the presence of reducing agents such as ascorbic acid and 2-mercaptoethanol. The products of 5'-monophosphate-2'-deoxyguanosine (dGMP) oxidation were directly analyzed using the HPLC-ESI/MS method. The main oxidation products were 5'-phospho-2'-deoxy-8-oxo-7,8-dihydroguanine and the 1,N2-glyoxal adduct of the 5'-monophosphate-2'-deoxyguanosine.
Kuznetsova
Aleksandra A
AA
Novosibirsk State University, 630090 Novosibirsk, Russia.
Solovyeva
Lyudmila I
LI
Kaliya
Oleg L
OL
Lukyanets
Evgeniy A
EA
Knorre
Dmitri G
DG
Fedorova
Olga S
OS
D000970:Antineoplastic Agents / Q000138:chemical synthesis / Q000494:pharmacology; D000998:Antiviral Agents / Q000494:pharmacology; D001205:Ascorbic Acid / Q000737:chemistry; D002384:Catalysis; D002626:Chemistry, Pharmaceutical / Q000379:methods; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003848:Deoxyguanine Nucleotides / Q000737:chemistry*; D015195:Drug Design; D006801:Humans; D017665:Hydroxyl Radical; D007211:Indoles / Q000737:chemistry*; D007501:Iron / Q000737:chemistry*; D054833:Isoindoles; D008623:Mercaptoethanol / Q000737:chemistry; D008956:Models, Chemical; D010100:Oxygen / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods
D000970:Antineoplastic Agents; D000998:Antiviral Agents; D003848:Deoxyguanine Nucleotides; D007211:Indoles; D054833:Isoindoles; D017665:Hydroxyl Radical; D008623:Mercaptoethanol; C007257:2'-deoxyguanosine 5'-phosphate; D007501:Iron; D001205:Ascorbic Acid; D010100:Oxygen; C013647:phthalocyanine