Association of tumor necrosis factor-alpha with fever and pulmonary lesion score in pigs experimentally infected with swine influenza virus subtype H1N2.
The relationship between tumor necrosis factor (TNF)-alpha level, body temperature, and pulmonary lesion score was determined in 3-week-old pigs inoculated intranasally with swine influenza virus (SIV) subtype H1N2. The expression of TNF-alpha was measured in bronchoalveolar lavage (BAL) fluids by enzyme-linked immunosorbent assay and lung tissues by immunohistochemistry. In BAL fluid, TNF-alpha concentration was maximal at 1 days post-inoculation (dpi), declined markedly by 3 dpi (P<0.05) and steady thereafter. Mean rectal temperatures were above 40 degrees C for the infected groups at 1 dpi and declined markedly by 3 dpi. The body temperatures were correlated with the levels of TNF-alpha in BAL fluids from pigs experimentally infected with SIV (r(s)=0.9754, P<0.05). The pulmonary lesion scores were correlated with the means of positive cells by immunohistochemistry for TNF- alpha (r(s)=0.9949, P<0.001). The results suggest that the expression of TNF-alpha protein plays an important role in induction of pulmonary lesion and clinical sign such as fever in SIV infection.
Kim
Bongtae
B
Department of Veterinary Pathology, Seoul National University, Seoul, Republic of Korea.
Ahn
Kyoung Kyu
KK
Ha
Yooncheol
Y
Lee
Yong Hoon
YH
Kim
Duyeol
D
Lim
Jeong Han
JH
Kim
Sung-Hoon
SH
Kim
Mi-Young
MY
Cho
Kyung-Dong
KD
Lee
Bog-Hieu
BH
Chae
Chanhee
C
D000818:Animals; D001831:Body Temperature / Q000276:immunology; D001992:Bronchoalveolar Lavage Fluid / Q000276:immunology / Q000821:virology; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D007150:Immunohistochemistry / Q000662:veterinary; D053118:Influenza A Virus, H1N1 Subtype / Q000235:genetics / Q000276:immunology*; D008171:Lung Diseases / Q000276:immunology / Q000662:veterinary* / Q000821:virology; D016676:Macrophages, Alveolar / Q000276:immunology / Q000821:virology; D009976:Orthomyxoviridae Infections / Q000276:immunology / Q000662:veterinary* / Q000821:virology; D011897:Random Allocation; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D013552:Swine; D013553:Swine Diseases / Q000276:immunology / Q000821:virology*; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis* / Q000235:genetics / Q000276:immunology
D014409:Tumor Necrosis Factor-alpha