Subunit a of the F(1)F(0) ATP synthase requires YidC and SecYEG for membrane insertion.
The insertion of inner membrane proteins in Escherichia coli occurs almost exclusively via the SecYEG pathway, while some membrane proteins require the membrane protein insertase YidC. In vitro analysis demonstrates that subunit a of the F(1)F(0) ATP synthase (F(0)a) is strictly dependent on Ffh, SecYEG and YidC for its membrane insertion but independent of the proton motive force. The insertion of the first transmembrane segment of F(0)a also depends on Ffh and SecYEG but not on YidC, whereas the insertion is strongly dependent on the proton motive force, unlike the full-length F(0)a protein. These data demonstrate an extensive role of YidC in the assembly of the F(0) sector of the F(1)F(0) ATP synthase.
Kol
Stefan
S
Department of Molecular Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Haren, The Netherlands.
Majczak
Wiktor
W
Heerlien
René
R
van der Berg
Jan Pieter
JP
Nouwen
Nico
N
Driessen
Arnold J M
AJ
D025243:Bacterial Proton-Translocating ATPases / Q000378:metabolism*; D002462:Cell Membrane / Q000201:enzymology*; D004926:Escherichia coli / Q000201:enzymology*; D029968:Escherichia coli Proteins / Q000378:metabolism*; D026901:Membrane Transport Proteins / Q000378:metabolism*; D008958:Models, Molecular; D050505:Mutant Proteins / Q000378:metabolism; D021122:Protein Subunits / Q000378:metabolism*; D011510:Proteolipids / Q000378:metabolism; D018892:Proton-Motive Force; D000069816:SEC Translocation Channels; D018271:Signal Recognition Particle / Q000378:metabolism
D029968:Escherichia coli Proteins; D026901:Membrane Transport Proteins; D050505:Mutant Proteins; D021122:Protein Subunits; D011510:Proteolipids; D000069816:SEC Translocation Channels; D018271:Signal Recognition Particle; C412443:YIDC protein, E coli; C020485:proteoliposomes; D025243:Bacterial Proton-Translocating ATPases