Chebulagic acid, a COX-LOX dual inhibitor isolated from the fruits of Terminalia chebula Retz., induces apoptosis in COLO-205 cell line.
BACKGROUND
Terminalia chebula has an esteemed origin in Indian mythology; its fruits are used to treat many diseases such as digestive, diabetes, colic pain, chronic cough, sore throat, asthma, etc.


OBJECTIVE
The water or ethanolic extracts of the fruits were reported to have anti-oxidant, anti-inflammatory, anti-cancer and radio-protector properties. The present study is to isolate and identify the compounds that inhibit COX and 5-LOX, the key enzymes involved in inflammation and carcinogenesis.


METHODS
The ethanolic extract of the fruits was fractionated by RP-HPLC and fractions were tested for enzyme inhibition activity against COX and 5-LOX. One of the fractionated compounds showed potent dual inhibition against COX and 5-LOX. It was identified as chebulagic acid by LC-MS, NMR and IR analyses. The chebulagic acid was also tested for anti-proliferative activity.


RESULTS
Chebulagic acid showed potent COX-LOX dual inhibition activity with IC(50) values of 15+/-0.288, 0.92+/-0.011 and 2.1+/-0.057 microM for COX-1, COX-2 and 5-LOX respectively. It also showed anti-proliferative activity against HCT-15, COLO-205, MDA-MB-231, DU-145 and K562 cell lines. Further mechanistic studies on COLO-205 cells revealed induction of apoptosis by chebulagic acid.


CONCLUSIONS
Chebulagic acid, a COX-2 and 5-LOX dual inhibitor isolated from the fruits of Terminalia chebula, induces apoptosis in COLO-205 cells.
Reddy
D Bharat
DB
Department of Animal Sciences, School of Life Sciences, University of Hyderabad, Hyderabad 500046, India.
Reddy
T C M
TC
Jyotsna
G
G
Sharan
Satish
S
Priya
Nalini
N
Lakshmipathi
V
V
Reddanna
Pallu
P
D000975:Antioxidants / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects*; D001094:Arachidonate 5-Lipoxygenase / Q000302:isolation & purification; D001578:Benzopyrans / Q000494:pharmacology*; D001713:Biphenyl Compounds / Q000737:chemistry; D015153:Blotting, Western; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002851:Chromatography, High Pressure Liquid; D052246:Cyclooxygenase 2 Inhibitors / Q000494:pharmacology; D016861:Cyclooxygenase Inhibitors / Q000494:pharmacology*; D053938:DNA Fragmentation / Q000187:drug effects; D005434:Flow Cytometry; D016166:Free Radical Scavengers / Q000737:chemistry; D005609:Free Radicals / Q000737:chemistry; D005638:Fruit / Q000737:chemistry; D005960:Glucosides / Q000494:pharmacology*; D006801:Humans; D007202:Indicators and Reagents; D016859:Lipoxygenase Inhibitors / Q000494:pharmacology*; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D010851:Picrates / Q000737:chemistry; D013055:Spectrophotometry, Infrared; D029766:Terminalia / Q000737:chemistry*
D000975:Antioxidants; D001578:Benzopyrans; D001713:Biphenyl Compounds; D052246:Cyclooxygenase 2 Inhibitors; D016861:Cyclooxygenase Inhibitors; D016166:Free Radical Scavengers; D005609:Free Radicals; D005960:Glucosides; D007202:Indicators and Reagents; D016859:Lipoxygenase Inhibitors; D010851:Picrates; C076178:chebulagic acid; C004931:1,1-diphenyl-2-picrylhydrazyl; D001094:Arachidonate 5-Lipoxygenase