Pronuclear DNA injection for the production of transgenic rats.
In the absence of germ-line-competent ES cells in the rat, pronuclear microinjection remains an essential tool to generate transgenic rat models. However, DNA microinjection procedures first developed for mouse do not provide scientists with satisfying results when applied to rat. Here we describe optimized procedures for rat with a special focus on rat embryo production, in vitro incubation and culture, DNA pronuclear injection, and the transfer of embryos into foster females.
Cozzi
Jean
J
genOway SA, 69362, Lyon, France.
Anegon
Ignacio
I
Braun
Valérie
V
Gross
Anne-Catherine
AC
Merrouche
Christel
C
Cherifi
Yacine
Y
D000818:Animals; D030801:Animals, Genetically Modified / Q000196:embryology*; D002467:Cell Nucleus / Q000235:genetics*; D004247:DNA / Q000008:administration & dosage*; D004624:Embryo Transfer; D004622:Embryo, Mammalian / Q000166:cytology / Q000502:physiology; D005260:Female; D018014:Gene Transfer Techniques / Q000662:veterinary*; D008297:Male; D008845:Microinjections / Q000379:methods*; D051381:Rats; D017207:Rats, Sprague-Dawley / Q000196:embryology* / Q000235:genetics; D019076:Transgenes / Q000502:physiology*; D015053:Zygote / Q000502:physiology
D004247:DNA