[Effects of low temperature on functions of learning, memory and activities of ATPase in brain tissue of mice].
OBJECTIVE
To explore the effects of low temperature on the functions of learning and memory and activities of ATPase in brain tissue of mice.


METHODS
120 mice were randomly divided into 3 groups of A, B, and C with different cold exposure time. After low temperature test, learning and memory ability and activities of ATPase in brain tissue of the mice were measured.


RESULTS
Compared with corresponding control group, in the test of learning ability, the total electric shock period [(41.00 +/- 12.06), (45.90 +/- 13.61) min], the total electric times (85.00 +/- 15.81, 89.00 +/- 17.29), and the error reaction times (33.60 +/- 10.69, 39.00 +/- 11.63) were increased in group A and group C significantly (P < 0.01, P < 0.05), but the rate of right reaction (53.60% +/- 11.23%, 54.59% +/- 6.14%) were decreased in group A and group C significantly (P < 0.01, P < 0.05). In the test of memory ability, the total electric shock period [(19.00 +/- 4.62), (51.70 +/- 15.19) min] in group A and group C were increased significantly (P < 0.05, P < 0.01), the rate of right reaction (86.17% +/- 6.34%, 65.92% +/- 8.17%) in group A and group C were decreased significantly (P < 0.05). In test of activities of ATPase in brain tissue, the activities of Na(+)-K(+)-ATPase and activities of Ca(2+)-Mg(2+)-ATPase in brain tissue in group A and group C were decreased significantly (P < 0.05, P < 0.01).


CONCLUSIONS
Low temperature could decrease the functions of learning and memory and the activities of Na+-K+-ATPase and Ca(2+)-Mg(2+)-ATPase in brain tissue of mice.
Han
Yun-mei
YM
Key Laboratory of Occupational Health and Environmental Medicine, Jining Medical College, Jining 272013, China.
Zhang
Chun-zhi
CZ
Lin
Li
L
Chen
Lei
L
D000818:Animals; D001921:Brain / Q000201:enzymology*; D017301:Ca(2+) Mg(2+)-ATPase / Q000378:metabolism*; D003080:Cold Temperature / Q000009:adverse effects*; D004781:Environmental Exposure / Q000009:adverse effects; D005260:Female; D008297:Male; D018782:Maze Learning; D008568:Memory; D051379:Mice; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism*
D017301:Ca(2+) Mg(2+)-ATPase; D000254:Sodium-Potassium-Exchanging ATPase