MicroRNA expression profiling in diabetic GK rat model.
MicroRNAs (miRNAs), which are a newly identified class of small single-stranded non-coding RNAs, regulate their target genes via post-transcriptional pathway. It has been proved that miRNAs play important roles in many biological processes. To better understand miRNA function on type 2 diabetes, we used an oligonucleotide microarray to monitor miRNA expression profiles of Goto-Kakizaki (GK) and Wistar rats' skeletal muscle. It was found that seven miRNAs were downexpressed and two miRNAs were over-expressed in the muscle of GK rats. Among them, miR-24 showed the most prominent change. p38 MAPK, which is a direct target of miR-24, also showed expression difference. All the data give a clue that miR-24 might be associated with diabetes through down-regulation of p38 MAPK.
Huang
Bing
B
State Key Laboratory of Molecular Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China.
Qin
Wenming
W
Zhao
Botao
B
Shi
Yi
Y
Yao
Chengguo
C
Li
Jin
J
Xiao
Huasheng
H
Jin
Youxin
Y
D000818:Animals; D001483:Base Sequence; D015153:Blotting, Western; D015342:DNA Probes; D003921:Diabetes Mellitus, Experimental / Q000235:genetics*; D004195:Disease Models, Animal; D020869:Gene Expression Profiling; D035683:MicroRNAs / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis; D051381:Rats; D017208:Rats, Wistar
D015342:DNA Probes; D035683:MicroRNAs