title: EWOD-driven droplet microfluidic device integrated with optoelectronic tweezers as an automated platform for cellular isolation and analysis.
abstract: We report the integration of two technologies: droplet microfluidics using electrowetting-on-dielectric (EWOD) and individual particle manipulation using optoelectronic tweezers (OET)-in one microfluidic device. The integrated device successfully demonstrates a sequence involving both EWOD and OET operations. We encountered various challenges during integration of the two different technologies and present how they are addressed. To show the applicability of the device in cellular biology, live HeLa cells are used in the experiments. The unique advantages of EWOD and OET make their integration a significant step towards a powerful tool for many applications, such as single cell studies involving multiplexed environmental stimuli.
authors.affiliation: UCLA, Engineering IV Bldg, Los Angeles, CA, USA. gjshah@ucla.edu
authors.forename: Gaurav J
authors.initials: GJ
authors.lastname: Shah
authors.forename: Aaron T
authors.initials: AT
authors.lastname: Ohta
authors.forename: Eric P-Y
authors.initials: EP
authors.lastname: Chiou
authors.forename: Ming C
authors.initials: MC
authors.lastname: Wu
authors.forename: Chang-Jin C J
authors.initials: CJ
authors.lastname: Kim
mesh_terms: D000388:Air; D001331:Automation; D002469:Cell Separation / Q000295:instrumentation*; D017097:Electric Impedance; D004560:Electricity; D004867:Equipment Design; D006367:HeLa Cells; D006801:Humans; D008280:Magnetics; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D052898:Optical Tweezers; D013499:Surface Properties; D013501:Surface-Active Agents / Q000737:chemistry; D018511:Systems Integration
chemical_list: D013501:Surface-Active Agents