title: Pronuclear DNA injection for the production of transgenic rats.
abstract: In the absence of germ-line-competent ES cells in the rat, pronuclear microinjection remains an essential tool to generate transgenic rat models. However, DNA microinjection procedures first developed for mouse do not provide scientists with satisfying results when applied to rat. Here we describe optimized procedures for rat with a special focus on rat embryo production, in vitro incubation and culture, DNA pronuclear injection, and the transfer of embryos into foster females.
authors.affiliation: genOway SA, 69362, Lyon, France.
authors.forename: Jean
authors.initials: J
authors.lastname: Cozzi
authors.forename: Ignacio
authors.initials: I
authors.lastname: Anegon
authors.forename: Valérie
authors.initials: V
authors.lastname: Braun
authors.forename: Anne-Catherine
authors.initials: AC
authors.lastname: Gross
authors.forename: Christel
authors.initials: C
authors.lastname: Merrouche
authors.forename: Yacine
authors.initials: Y
authors.lastname: Cherifi
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified / Q000196:embryology*; D002467:Cell Nucleus / Q000235:genetics*; D004247:DNA / Q000008:administration & dosage*; D004624:Embryo Transfer; D004622:Embryo, Mammalian / Q000166:cytology / Q000502:physiology; D005260:Female; D018014:Gene Transfer Techniques / Q000662:veterinary*; D008297:Male; D008845:Microinjections / Q000379:methods*; D051381:Rats; D017207:Rats, Sprague-Dawley / Q000196:embryology* / Q000235:genetics; D019076:Transgenes / Q000502:physiology*; D015053:Zygote / Q000502:physiology
chemical_list: D004247:DNA