[Fluorescent linear labelling method for biochip applications].
Fluorescent Linear Labelling technique is an effective, single-tube and linear amplification method. The sample cDNA was synthesized from total RNA, and was labelled antisense cRNA from double-stranded cDNA with T7RNA polymerase; it can be used for hybridization to oligonucleotide biochip. Fluorescent Linear Labelling Method can result in 50- to 100-fold higher degrees of amplification, and has been shown to retain information on transcript abundance, thus making it an efficient, robust technique for fluorescent labelling on biochip.
Ding
Dapeng
D
Institute of Genetic Engineering, Nanfang Medical University, Guangzhou 510515, China.
Ma
Wenli
W
Zhang
Ying
Y
Shi
Rong
R
Zhou
Jueyu
J
Zheng
Wenling
W
D005456:Fluorescent Dyes / Q000737:chemistry*; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D013194:Staining and Labeling / Q000379:methods*
D005456:Fluorescent Dyes