title: Hydrogen mediates suppression of colon inflammation induced by dextran sodium sulfate.
abstract: By its antioxidant effect, molecular hydrogen gas (H2) was reported to protect organs from tissue damage induced by ischemia reperfusion. To evaluate its anti-inflammatory effects, we established a mouse model of human inflammatory bowel disease (IBD) by supplying mice with water containing (1) dextran sodium sulfate (DSS) (5%), (2) DSS (5%) and H2, or (3) H2 only ad libitum up to 7 days. At day-7, DSS-induced pathogenic outcomes including, loss of body weight, increase of colitis score, pathogenic shortening of colon length, elevated level of IL-12, TNF-alpha and IL-1beta in colon lesion, were significantly suppressed by the addition of H2 to DSS solution. Histological analysis also revealed that the DSS-mediated colonic tissue destruction accompanied by macrophage infiltration was remarkably suppressed by H2. Therefore, the present study indicated that H2 can prevent the development of DSS-induced colitis in mice.
authors.affiliation: Department of Immunology, The Forsyth Institute, Boston, MA 02115, USA.
authors.forename: Mikihito
authors.initials: M
authors.lastname: Kajiya
authors.forename: Marcelo J B
authors.initials: MJ
authors.lastname: Silva
authors.forename: Kimihiro
authors.initials: K
authors.lastname: Sato
authors.forename: Kazuhisa
authors.initials: K
authors.lastname: Ouhara
authors.forename: Toshihisa
authors.initials: T
authors.lastname: Kawai
mesh_terms: D000280:Administration, Inhalation; D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage*; D003092:Colitis / Q000139:chemically induced / Q000188:drug therapy* / Q000276:immunology / Q000473:pathology; D003106:Colon / Q000187:drug effects* / Q000276:immunology / Q000473:pathology; D016207:Cytokines / Q000096:biosynthesis; D016264:Dextran Sulfate / Q000633:toxicity; D004195:Disease Models, Animal; D015536:Down-Regulation; D006801:Humans; D006859:Hydrogen / Q000008:administration & dosage*; D008264:Macrophages / Q000187:drug effects / Q000276:immunology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C
chemical_list: D000975:Antioxidants; D016207:Cytokines; D006859:Hydrogen; D016264:Dextran Sulfate