title: Studies on endophytic colonization ability of two upland rice endophytes, Rhizobium sp. and Burkholderia sp., using green fluorescent protein reporter.
abstract: Colonization ability of the two endophytic bacteria, isolated from surface sterilized roots of upland cultivated rice viz., Rhizobium sp. and Burkholderia sp., was compared after genetically tagging them with a constitutively expressing green fluorescent protein gene (gfp/gusA). Confocal laser scanning microscopy (CLSM) of gnotobiotically grown seedlings of Narendradhan 97, inoculated with gfp/gusA-tagged endophytes, revealed that both Rhizobium sp. and Burkholderia sp. colonized the intercellular spaces in the root cortex when inoculated separately. Colonization by gfp/gusA-tagged Rhizobium sp. was severely inhibited when co-inoculated with an equal number (10(6) cfu ml(-1)) of wild type Burkholderia sp. Burkholderia sp. was a more aggressive endophytic colonizer of rice than Rhizobium sp. The potential of using gfp/gusA reporter and CLSM as tools in evaluating competitive ability of colonization among endophytes is demonstrated in this study.
authors.affiliation: Microbial Genetics Laboratory, Genetics and Plant Breeding, Institute of Agricultural Sciences, Banaras Hindu University, Varanasi, 221005, India. mintusingh78@gmail.com
authors.forename: Manoj Kumar
authors.initials: MK
authors.lastname: Singh
authors.forename: Chanda
authors.initials: C
authors.lastname: Kushwaha
authors.forename: Ramesh Kumar
authors.initials: RK
authors.lastname: Singh
mesh_terms: D000898:Antibiosis; D019117:Burkholderia / Q000254:growth & development* / Q000302:isolation & purification; D017930:Genes, Reporter / Q000235:genetics; D049452:Green Fluorescent Proteins / Q000378:metabolism; D018613:Microscopy, Confocal; D012275:Oryza / Q000382:microbiology*; D018517:Plant Roots / Q000382:microbiology*; D012231:Rhizobium / Q000254:growth & development* / Q000302:isolation & purification; D013194:Staining and Labeling / Q000379:methods
chemical_list: D049452:Green Fluorescent Proteins