title: Molecular cloning, expression and characterization of a novel mouse SULT6 cytosolic sulfotransferase.
abstract: By searching the mouse EST database, we identified a novel mouse cytosolic sulfotransferase (SULT) cDNA (RIKEN cDNA 2410078J06). Sequence analysis revealed that this new SULT belongs to the cytosolic SULT6 gene family. The recombinant form of this newly identified SULT, designated SULT6B1, was expressed using the pGEX-4T-1 glutathione S-transferase fusion system and purified from transformed BL21 Escherichia coli cells. Purified mouse SULT6B1 exhibited sulfonating activity toward thyroxine and bithionol among a variety of endogenous and xenobiotic compounds tested as substrates. pH optimum of purified mouse SULT6B1 was determined to be 8.0. Tissue-specific expression of mouse and human SULT6B1 was examined by RT-PCR. While human SULT6B1 was specifically expressed in kidney and testis, mouse SULT6B1 was detected in brain, heart, kidney, thymus, lung, liver and testis. Further studies are needed in order to clarify the role of SULT6B1 in the metabolism of thyroxine and possibly some xenobiotics in mouse.
authors.affiliation: Department of Biochemistry, University of Miyazaki, Miyazaki, Miyazaki 889-2192, Japan.
authors.forename: Saki
authors.initials: S
authors.lastname: Takahashi
authors.forename: Yoichi
authors.initials: Y
authors.lastname: Sakakibara
authors.forename: Emi
authors.initials: E
authors.lastname: Mishiro
authors.forename: Haruna
authors.initials: H
authors.lastname: Kouriki
authors.forename: Rika
authors.initials: R
authors.lastname: Nobe
authors.forename: Katsuhisa
authors.initials: K
authors.lastname: Kurogi
authors.forename: Shin
authors.initials: S
authors.lastname: Yasuda
authors.forename: Ming-Cheh
authors.initials: MC
authors.lastname: Liu
authors.forename: Masahito
authors.initials: M
authors.lastname: Suiko
mesh_terms: D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D000818:Animals; D001735:Bithionol / Q000378:metabolism; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000378:metabolism; D020224:Expressed Sequence Tags; D005260:Female; D015870:Gene Expression; D015723:Gene Library; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D009206:Myocardium / Q000201:enzymology; D009928:Organ Specificity; D010724:Phosphoadenosine Phosphosulfate / Q000378:metabolism; D010802:Phylogeny; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D013379:Substrate Specificity; D015238:Sulfotransferases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D013974:Thyroxine / Q000378:metabolism
chemical_list: D018076:DNA, Complementary; D011993:Recombinant Fusion Proteins; D010724:Phosphoadenosine Phosphosulfate; D001735:Bithionol; C544761:SULT6B1 protein, human; C544760:SULT6B1 protein, mouse; D015238:Sulfotransferases; D013974:Thyroxine