An alternative method for DNA extraction and PCR identification of Entamoeba histolytica and E. dispar in fecal samples.
Since it is known that Entamoeba dispar is non-pathogenic and morphologically similar to E. histolytica, there are many targets used in PCR for differentiating these species. However, obtaining high quality DNA from fecal samples is fundamental for PCR. Most methods are laborious or use kits that make diagnosis expensive. In the present work, a new simple, fast and cheap technique of DNA extraction from fecal samples was combined with a PCR for an episomal target in order to identify E. histolytica and E. dispar in feces.
Vianna
E N
EN
Departamento de Parasitologia, ICB, Universidade Federal de Minas Gerais, Av. Antonio Carlos, 6627-31270-901, Belo Horizonte, Minas Gerais, Brazil.
Costa
J O
JO
Santos
C K S
CK
Cury
M C
MC
Silva
E F
EF
Costa
A O
AO
Gomes
M A
MA
D000818:Animals; D016054:DNA, Protozoan / Q000032:analysis / Q000302:isolation & purification*; D004747:Entamoeba / Q000145:classification* / Q000235:genetics; D004748:Entamoeba histolytica / Q000145:classification* / Q000235:genetics; D004749:Entamoebiasis / Q000175:diagnosis / Q000469:parasitology; D005243:Feces / Q000469:parasitology*; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D011933:Reagent Kits, Diagnostic; D013045:Species Specificity
D016054:DNA, Protozoan; D011933:Reagent Kits, Diagnostic