Studies on endophytic colonization ability of two upland rice endophytes, Rhizobium sp. and Burkholderia sp., using green fluorescent protein reporter.
Colonization ability of the two endophytic bacteria, isolated from surface sterilized roots of upland cultivated rice viz., Rhizobium sp. and Burkholderia sp., was compared after genetically tagging them with a constitutively expressing green fluorescent protein gene (gfp/gusA). Confocal laser scanning microscopy (CLSM) of gnotobiotically grown seedlings of Narendradhan 97, inoculated with gfp/gusA-tagged endophytes, revealed that both Rhizobium sp. and Burkholderia sp. colonized the intercellular spaces in the root cortex when inoculated separately. Colonization by gfp/gusA-tagged Rhizobium sp. was severely inhibited when co-inoculated with an equal number (10(6) cfu ml(-1)) of wild type Burkholderia sp. Burkholderia sp. was a more aggressive endophytic colonizer of rice than Rhizobium sp. The potential of using gfp/gusA reporter and CLSM as tools in evaluating competitive ability of colonization among endophytes is demonstrated in this study.
Singh
Manoj Kumar
MK
Microbial Genetics Laboratory, Genetics and Plant Breeding, Institute of Agricultural Sciences, Banaras Hindu University, Varanasi, 221005, India. mintusingh78@gmail.com
Kushwaha
Chanda
C
Singh
Ramesh Kumar
RK
D000898:Antibiosis; D019117:Burkholderia / Q000254:growth & development* / Q000302:isolation & purification; D017930:Genes, Reporter / Q000235:genetics; D049452:Green Fluorescent Proteins / Q000378:metabolism; D018613:Microscopy, Confocal; D012275:Oryza / Q000382:microbiology*; D018517:Plant Roots / Q000382:microbiology*; D012231:Rhizobium / Q000254:growth & development* / Q000302:isolation & purification; D013194:Staining and Labeling / Q000379:methods
D049452:Green Fluorescent Proteins