Mouririelliptica: validation of gastroprotective, healing and anti-Helicobacter pylori effects.
BACKGROUND
Mouriri elliptica Martius (Melastomataceae) is species reputed in folk medicine to heal gastric ulcer and gastritis.


OBJECTIVE
Methanolic extract (ME) and ethyl acetate fraction (EAF) from leaves of Mouriri elliptica were evaluated for their gastroprotective, healing, immunological, toxicological and anti-Helicobacter pylori activities.


METHODS
The gastroprotective action of ME and EAF was evaluated in rodent experimental models and to elucidate mechanisms of action, the antisecretory action, involvements of NO, SH, PGE(2), anti-Helicobacter pylori action of ME was evaluated. We also used immunohistochemical (PCNA and COX-2) and immunomodulatory (murine peritoneal macrophages) assays to evaluate Mouriri elliptica effects.


RESULTS
ME present gastroprotective action without antisecretory effect. Otherwise, ME showed anti-Helicobacter pylori action (MIC=0.025mug/mL) and was able to inhibit NO production by macrophages. This species also accelerate the healing of ulcerated gastric mucosa by stimulating proliferation factors (PCNA), COX-2 and maintained basal PGE(2) level independent action of NSAID in gastric mucosa. The phytochemical investigation showed that this species possesses phenolic acid derivatives, acylglycoflavonoids and condensed tannins which probably influenced their pharmacological action.


CONCLUSIONS
All these results suggest the efficacy and safety of Mouriri elliptica in combating and healing gastric ulcer.
Moleiro
Fábio Cruz
FC
São Paulo State University-Departamento de Fisiologia, Instituto de Biociências, Cp 610, CEP 18618-000, UNESP, Botucatu, SP, Brazil.
Andreo
Márcio Aparecido
MA
Santos
Raquel de Cássia Dos
Rde C
Moraes
Thiago de Mello
Tde M
Rodrigues
Clenilson Martins
CM
Carli
Camila Bernardes de Andrade
CB
Lopes
Flávia Cristine Mascia
FC
Pellizzon
Cláudia Helena
CH
Carlos
Iracilda Zeppone
IZ
Bauab
Tais Maria
TM
Vilegas
Wagner
W
Hiruma-Lima
Clélia Akiko
CA
D000818:Animals; D000900:Anti-Bacterial Agents / Q000009:adverse effects / Q000494:pharmacology / Q000627:therapeutic use; D000894:Anti-Inflammatory Agents, Non-Steroidal; D000897:Anti-Ulcer Agents / Q000009:adverse effects / Q000494:pharmacology / Q000627:therapeutic use*; D051546:Cyclooxygenase 2 / Q000378:metabolism; D015232:Dinoprostone / Q000378:metabolism; D004195:Disease Models, Animal; D000431:Ethanol; D005260:Female; D005419:Flavonoids / Q000009:adverse effects / Q000494:pharmacology / Q000627:therapeutic use; D005753:Gastric Mucosa / Q000187:drug effects* / Q000378:metabolism; D016481:Helicobacter Infections / Q000139:chemically induced / Q000188:drug therapy*; D016480:Helicobacter pylori / Q000187:drug effects*; D008264:Macrophages / Q000187:drug effects; D008297:Male; D035322:Melastomataceae / Q000737:chemistry; D051379:Mice; D009569:Nitric Oxide / Q000037:antagonists & inhibitors; D010636:Phenols / Q000009:adverse effects / Q000494:pharmacology / Q000627:therapeutic use; D008517:Phytotherapy; D010936:Plant Extracts / Q000009:adverse effects / Q000494:pharmacology / Q000627:therapeutic use*; D018515:Plant Leaves; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism; D051381:Rats; D013276:Stomach Ulcer / Q000139:chemically induced / Q000188:drug therapy* / Q000382:microbiology; D013634:Tannins / Q000009:adverse effects / Q000494:pharmacology / Q000627:therapeutic use
D000900:Anti-Bacterial Agents; D000894:Anti-Inflammatory Agents, Non-Steroidal; D000897:Anti-Ulcer Agents; D005419:Flavonoids; D010636:Phenols; D010936:Plant Extracts; D018809:Proliferating Cell Nuclear Antigen; D013634:Tannins; D009569:Nitric Oxide; D000431:Ethanol; D051546:Cyclooxygenase 2; D015232:Dinoprostone