title: Subunit a of the F(1)F(0) ATP synthase requires YidC and SecYEG for membrane insertion.
abstract: The insertion of inner membrane proteins in Escherichia coli occurs almost exclusively via the SecYEG pathway, while some membrane proteins require the membrane protein insertase YidC. In vitro analysis demonstrates that subunit a of the F(1)F(0) ATP synthase (F(0)a) is strictly dependent on Ffh, SecYEG and YidC for its membrane insertion but independent of the proton motive force. The insertion of the first transmembrane segment of F(0)a also depends on Ffh and SecYEG but not on YidC, whereas the insertion is strongly dependent on the proton motive force, unlike the full-length F(0)a protein. These data demonstrate an extensive role of YidC in the assembly of the F(0) sector of the F(1)F(0) ATP synthase.
authors.affiliation: Department of Molecular Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Haren, The Netherlands.
authors.forename: Stefan
authors.initials: S
authors.lastname: Kol
authors.forename: Wiktor
authors.initials: W
authors.lastname: Majczak
authors.forename: René
authors.initials: R
authors.lastname: Heerlien
authors.forename: Jan Pieter
authors.initials: JP
authors.lastname: van der Berg
authors.forename: Nico
authors.initials: N
authors.lastname: Nouwen
authors.forename: Arnold J M
authors.initials: AJ
authors.lastname: Driessen
mesh_terms: D025243:Bacterial Proton-Translocating ATPases / Q000378:metabolism*; D002462:Cell Membrane / Q000201:enzymology*; D004926:Escherichia coli / Q000201:enzymology*; D029968:Escherichia coli Proteins / Q000378:metabolism*; D026901:Membrane Transport Proteins / Q000378:metabolism*; D008958:Models, Molecular; D050505:Mutant Proteins / Q000378:metabolism; D021122:Protein Subunits / Q000378:metabolism*; D011510:Proteolipids / Q000378:metabolism; D018892:Proton-Motive Force; D000069816:SEC Translocation Channels; D018271:Signal Recognition Particle / Q000378:metabolism
chemical_list: D029968:Escherichia coli Proteins; D026901:Membrane Transport Proteins; D050505:Mutant Proteins; D021122:Protein Subunits; D011510:Proteolipids; D000069816:SEC Translocation Channels; D018271:Signal Recognition Particle; C412443:YIDC protein, E coli; C020485:proteoliposomes; D025243:Bacterial Proton-Translocating ATPases