[Effects of precursor feeding and fungal elicitors on secondary metabolits in cell suspension culture of Cistanche deserticola].
OBJECTIVE
To investigate the effects of precursor feeding and fungal elicitors on cell growth and the contents of PeGs and Echin in the cell supension culture of Cistanche deserticola.


METHODS
The combination of precursor feeding and fungal elicitors were added on the cell suspension culture.


RESULTS
The combination of L-phenylalanine and L-Tyrosing were added with Cladosporium fulvum, the dry weight of cell and the contents of PeGs and Echin in suspention all reached the highest value, 14.69 gDW/L, 50.55 mg/g and 23.86 mg/g, which were 1.81, 4.18 and 3.99 times as much as the values of controls, respectively.


CONCLUSIONS
Precursor feeding and fungal elicitors had obvious promotion effects on cell growth and contents of PeGs and Echin in cell suspension culture of C. deserticola.
Lv
Jian-jun
JJ
School of Traditional Chinese Meteria Medica, Shenyang Pharmaceutical University, Shenyang 110016, China.
Hu
Gao-sheng
GS
Li
Jing-kui
JK
Jia
Jing-ming
JM
D018929:Cell Culture Techniques; D002455:Cell Division / Q000187:drug effects / Q000502:physiology; D002478:Cells, Cultured; D031671:Cistanche / Q000166:cytology / Q000378:metabolism* / Q000502:physiology; D003470:Culture Media; D004305:Dose-Response Relationship, Drug; D006027:Glycosides / Q000096:biosynthesis*; D010649:Phenylalanine / Q000494:pharmacology*; D014443:Tyrosine / Q000494:pharmacology*
D003470:Culture Media; D006027:Glycosides; D014443:Tyrosine; D010649:Phenylalanine; C060297:echinacoside