title: A human glomerular SAGE transcriptome database.
abstract: BACKGROUND
  To facilitate in the identification of gene products important in regulating renal glomerular structure and function, we have produced an annotated transcriptome database for normal human glomeruli using the SAGE approach.
  
  
  METHODS
  The database contains 22,907 unique SAGE tag sequences, with a total tag count of 48,905. For each SAGE tag, the ratio of its frequency in glomeruli relative to that in 115 non-glomerular tissues or cells, a measure of transcript enrichment in glomeruli, was calculated. A total of 133 SAGE tags representing well-characterized transcripts were enriched 10-fold or more in glomeruli compared to other tissues. Comparison of data from this study with a previous human glomerular Sau3A-anchored SAGE library reveals that 47 of the highly enriched transcripts are common to both libraries. Among these are the SAGE tags representing many podocyte-predominant transcripts like WT-1, podocin and synaptopodin. Enrichment of podocyte transcript tags SAGE library indicates that other SAGE tags observed at much higher frequencies in this glomerular compared to non-glomerular SAGE libraries are likely to be glomerulus-predominant. A higher level of mRNA expression for 19 transcripts represented by glomerulus-enriched SAGE tags was verified by RT-PCR comparing glomeruli to lung, liver and spleen.
  
  
  CONCLUSIONS
  The database can be retrieved from, or interrogated online at http://cgap.nci.nih.gov/SAGE. The annotated database is also provided as an additional file with gene identification for 9,022, and matches to the human genome or transcript homologs in other species for 1,433 tags. It should be a useful tool for in silico mining of glomerular gene expression.
authors.affiliation: Department of Medicine, University of Alberta, Edmonton, Alberta, Canada. Jenny.Nystroem@wlab.gu.se
authors.forename: Jenny
authors.initials: J
authors.lastname: Nyström
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Fierlbeck
authors.forename: Anna
authors.initials: A
authors.lastname: Granqvist
authors.forename: Stephen C
authors.initials: SC
authors.lastname: Kulak
authors.forename: Barbara J
authors.initials: BJ
authors.lastname: Ballermann
mesh_terms: D000368:Aged; D000951:Antigens, Neoplasm / Q000235:genetics* / Q000378:metabolism*; D030541:Databases, Genetic; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics / Q000378:metabolism; D007678:Kidney Glomerulus / Q000378:metabolism*; D008297:Male; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism; D008840:Microfilament Proteins / Q000235:genetics / Q000378:metabolism; D008875:Middle Aged; D025721:WT1 Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D000951:Antigens, Neoplasm; D047908:Intracellular Signaling Peptides and Proteins; D008565:Membrane Proteins; D008840:Microfilament Proteins; C407223:NPHS2 protein; C412325:SAGE1 protein, human; C503967:SYNPO protein, human; D025721:WT1 Proteins