X-ray spectromicroscopy study of protein adsorption to a polystyrene-polylactide blend.
Synchrotron-based X-ray photoemission electron microscopy (X-PEEM) was used to study the adsorption of human serum albumin (HSA) to polystyrene-polylactide (40:60 PS-PLA, 0.7 wt %) thin films, annealed under various conditions. The rugosity of the substrate varied from 35 to 90 nm, depending on the annealing conditions. However, the characteristics of the protein adsorption (amounts and phase preference) were not affected by the changes in topography. The adsorption was also not changed by the phase inversion which occurred when the PS-PLA substrate was annealed above T(g) of the PLA. The amount of protein adsorbed depended on whether adsorption took place from distilled water or phosphate buffered saline solution. These differences are interpreted as a result of ionic strength induced changes in the protein conformation in solution.
Leung
Bonnie O
BO
BIMR, McMaster University, Hamilton, Ontario, Canada L8S 4M1. leungbo@mcmaster.ca
Hitchcock
Adam P
AP
Cornelius
Rena
R
Brash
John L
JL
Scholl
Andreas
A
Doran
Andrew
A
D000327:Adsorption; D006801:Humans; D008854:Microscopy, Electron / Q000295:instrumentation / Q000379:methods*; D009994:Osmolar Concentration; D011091:Polyesters / Q000737:chemistry*; D011137:Polystyrenes / Q000737:chemistry*; D011506:Proteins / Q000737:chemistry*; D012709:Serum Albumin / Q000737:chemistry; D012996:Solutions / Q000737:chemistry; D013499:Surface Properties; D017356:Synchrotrons; D014965:X-Rays
D011091:Polyesters; D011137:Polystyrenes; D011506:Proteins; D012709:Serum Albumin; D012996:Solutions; C033616:poly(lactide)