title: Upregulation of CENP-H in tongue cancer correlates with poor prognosis and progression.
abstract: BACKGROUND
  Centromere protein H (CENP-H) is one of the fundamental components of the human active kinetochore. Recently, CENP-H was identified to be associated with tumorigenesis. This study was aimed to investigate the clinicopathologic significance of CENP-H in tongue cancer.
  
  
  METHODS
  RT-PCR, real time RT-PCR and Western blot were used to examine the expression of CENP-H in tongue cancer cell lines and biopsies. CENP-H protein level in paraffin-embedded tongue cancer tissues were tested by immunohistochemical staining and undergone statistical analysis. CENP-H-knockdown stable cell line was established by infecting cells with a retroviral vector pSuper-retro-CENP-H-siRNA. The biological function of CENP-H was tested by MTT assay, colony formation assay, and Bromodeoxyuridine (BrdU) incorporation assay.
  
  
  RESULTS
  CENP-H expression was higher in tongue cancer cell lines and cancer tissues (T) than that in normal cell and adjacent noncancerous tongue tissues (N), respectively. It was overexpressed in 55.95% (94/168) of the paraffin-embedded tongue cancer tissues, and there was a strong correlation between CENP-H expression and clinical stage, as well as T classification. CENP-H can predict the prognosis of tongue cancer patients especially those in early stage. Depletion of CENP-H can inhibit the proliferation of tongue cancer cells (Tca8113) and downregulate the expression of Survivin.
  
  
  CONCLUSIONS
  These findings suggested that CENP-H involves in the development and progression of tongue cancer. CENP-H might be a valuable prognostic indicator for tongue cancer patients within early stage.
authors.affiliation: Department of Oral & Maxillofacial Surgery, The Second Affiliated Hospital of Sun Yat-sen University, Guangzhou, PR China. liaowt2002@gmail.com
authors.forename: Wen-Ting
authors.initials: WT
authors.lastname: Liao
authors.forename: Chun-Ping
authors.initials: CP
authors.lastname: Yu
authors.forename: Dong-Hui
authors.initials: DH
authors.lastname: Wu
authors.forename: Ling
authors.initials: L
authors.lastname: Zhang
authors.forename: Li-Hua
authors.initials: LH
authors.lastname: Xu
authors.forename: Gui-Xiang
authors.initials: GX
authors.lastname: Weng
authors.forename: Mu-Sheng
authors.initials: MS
authors.lastname: Zeng
authors.forename: Li-Bing
authors.initials: LB
authors.lastname: Song
authors.forename: Jin-Song
authors.initials: JS
authors.lastname: Li
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D017209:Apoptosis; D014408:Biomarkers, Tumor / Q000235:genetics* / Q000378:metabolism; D015153:Blotting, Western; D049109:Cell Proliferation; D002868:Chromosomal Proteins, Non-Histone / Q000235:genetics* / Q000378:metabolism; D003114:Colony-Forming Units Assay; D018450:Disease Progression; D005260:Female; D005455:Fluorescent Antibody Technique; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics*; D006801:Humans; D007124:Immunoenzyme Techniques; D008297:Male; D008875:Middle Aged; D011379:Prognosis; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015996:Survival Rate; D014059:Tongue / Q000378:metabolism / Q000473:pathology; D014062:Tongue Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D014162:Transfection; D015854:Up-Regulation
chemical_list: D014408:Biomarkers, Tumor; C121933:CENPH protein, human; D002868:Chromosomal Proteins, Non-Histone; D012333:RNA, Messenger