title: Modulating glycosidase degradation and lectin recognition of gold glyconanoparticles.
abstract: Glyconanoparticles (GNPs) are water-soluble carbohydrate-functionalized gold nanoclusters with a promising potential to serve as versatile tools in studies ranging from basic chemical glycobiology to clinical applications. In this paper we evaluate the influence of ligand density and presentation on the recognition by protein receptors by examining the interaction of lactose-functionalized GNPs with two different galactose-specific carbohydrate-binding proteins: an enzyme, Escherichia coli beta-galactosidase, and a lectin, Viscum album agglutinin. The results suggest that the proper selection of ligand densities and spacers in GNP functionalization is an important requisite to match the topological requirements of the target receptor while escaping glycosidase degradation.
authors.affiliation: Laboratory of Glyconanotechnology, IIQ-CSIC, Seville, Spain.
authors.forename: Africa G
authors.initials: AG
authors.lastname: Barrientos
authors.forename: Jesús M
authors.initials: JM
authors.lastname: de la Fuente
authors.forename: Marta
authors.initials: M
authors.lastname: Jiménez
authors.forename: Dolores
authors.initials: D
authors.lastname: Solís
authors.forename: F Javier
authors.initials: FJ
authors.lastname: Cañada
authors.forename: Manuel
authors.initials: M
authors.lastname: Martín-Lomas
authors.forename: Soledad
authors.initials: S
authors.lastname: Penadés
mesh_terms: D000373:Agglutinins / Q000378:metabolism*; D002240:Carbohydrate Sequence; D029968:Escherichia coli Proteins / Q000378:metabolism; D006046:Gold / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry*; D008962:Models, Theoretical; D008969:Molecular Sequence Data; D037121:Plant Lectins / Q000378:metabolism*; D011485:Protein Binding; D028182:Viscum album / Q000378:metabolism; D001616:beta-Galactosidase / Q000378:metabolism*
chemical_list: D000373:Agglutinins; D029968:Escherichia coli Proteins; D037121:Plant Lectins; D006046:Gold; D001616:beta-Galactosidase