Time course of Newcastle disease virus-induced apoptotic pathways.
Newcastle disease virus (NDV) causes economically significant Newcastle disease (ND) in almost all birds worldwide. Previous studies have shown that NDV induces caspase dependent apoptotic pathways in infected cells. In the present study, time course induction of apoptotic pathways in Vero cells is described. In NDV-infected cells, caspase-8 activity, percentage of cells showing TRAIL expression was higher at 24h p.i. (post-infection) compared to 48 h p.i. In contrast, caspase-9 activity, efflux of cytochrome c, loss of mitochondrial membrane potential was higher at 48 h compared to 24h p.i. The caspase-3 activity was high both times. Based on these results, it was concluded that at 24h p.i., NDV induces apoptosis through extrinsic apoptotic pathway while at 48 h p.i. predominantly through intrinsic apoptotic pathway.
Ravindra
P V
PV
Molecular Biology Laboratory, Division of Animal Biotechnology, Indian Veterinary Research Institute, Izatnagar 243122, UP, India. raviravindra1@gmail.com
Tiwari
Ashok K
AK
Ratta
Barkha
B
Bais
Manish V
MV
Chaturvedi
Uttara
U
Palia
Sudesh Kumar
SK
Sharma
Bhaskar
B
Chauhan
R S
RS
D000818:Animals; D017209:Apoptosis; D053181:Caspase 8 / Q000378:metabolism; D002522:Chlorocebus aethiops; D045304:Cytochromes c / Q000032:analysis; D003593:Cytoplasm / Q000737:chemistry; D020869:Gene Expression Profiling; D053078:Membrane Potential, Mitochondrial / Q000502:physiology; D009522:Newcastle disease virus / Q000472:pathogenicity*; D053221:TNF-Related Apoptosis-Inducing Ligand / Q000378:metabolism; D013997:Time Factors; D014709:Vero Cells
D053221:TNF-Related Apoptosis-Inducing Ligand; D045304:Cytochromes c; D053181:Caspase 8