title: Protein interaction platforms: visualization of interacting proteins in yeast.
abstract: Here we describe the protein interaction platform assay, a method for identifying interacting proteins in Saccharomyces cerevisiae. This assay relies on the reovirus scaffolding protein microNS, which forms large focal inclusions in living cells. When a query protein is fused to microNS and potential interaction partners are fused to a fluorescent reporter, interactors can be identified by screening for yeast that display fluorescent foci.
authors.affiliation: Department of Medicine (Microbiology and Molecular Genetics), Division of Infectious Diseases, Massachusetts General Hospital and Harvard Medical School, Cambridge, Massachusetts, USA.
authors.forename: Alexa M
authors.initials: AM
authors.lastname: Schmitz
authors.forename: Monica F
authors.initials: MF
authors.lastname: Morrison
authors.forename: Akochi O
authors.initials: AO
authors.lastname: Agunwamba
authors.forename: Max L
authors.initials: ML
authors.lastname: Nibert
authors.forename: Cammie F
authors.initials: CF
authors.lastname: Lesser
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D009154:Mutation; D010957:Plasmids / Q000235:genetics; D025941:Protein Interaction Mapping / Q000379:methods*; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*; D012486:Salmonella typhimurium / Q000235:genetics / Q000472:pathogenicity; D012763:Shigella flexneri / Q000235:genetics / Q000472:pathogenicity; D014774:Virulence / Q000235:genetics
chemical_list: D001426:Bacterial Proteins; D011993:Recombinant Fusion Proteins; D029701:Saccharomyces cerevisiae Proteins