title: Structural characterization of the lipopolysaccharide O-antigen from atypical isolate of Vibrio anguillarum strain 1282.
abstract: Vibrio anguillarum is a Gram-negative bacterium associated with vibriosis in Atlantic cod (Gadus morhua L.). Although farmed cod in Norway is routinely vaccinated against the infection, outbreaks of V. anguillarum-associated vibriosis still occur. Here, we describe the structural characterization of the LPS O-chain polysaccharide (O-PS) from atypical isolates of V. anguillarum strain 1282 and show that it is distinct from that previously established for V. anguillarum serotype O2. The structure of the purified O-PS was shown by 1D/2D NMR ((1)H, (13)C) spectroscopy and CE-MS studies to be a high-molecular mass linear polymer of tetrasaccharide repeating units, composed of 2-acetamido-3-(N-formyl-L-alanyl)amido-2,3-dideoxy-D-glucuronamide [GlcNAc3N(Fo-L-Ala)AN], 2-acetamido-3-acetamidino-2,3-dideoxy-D-mannuronic acid (ManNAc3NAmA), 3-acetamido-3-dideoxy-D-quinovose (Qui3NAc), and 2,4-diacetamido-2,4-dideoxy-D-fucose (FucNAc4NAc). [carbohydrate structure: see text]. NMR analysis of the partial hydrolysis-derived oligosaccharides confirmed the presence of an O-acetyl group at position O-4 of GlcNAc3N(Fo-L-Ala)AN and established that the above-mentioned structure represents the biological repeating unit of the O-PS. In addition, it was demonstrated that some of 2,3-diamino-2,3-dideoxy-glucuronamide in the O-PS was present in the form of 2,3-diamino-2,3-dideoxy-glucose.
authors.affiliation: Institute for Biological Sciences, National Research Council of Canada, Ottawa, Ontario, Canada K1A 0R6.
authors.forename: Zhan
authors.initials: Z
authors.lastname: Wang
authors.forename: Evgeny
authors.initials: E
authors.lastname: Vinogradov
authors.forename: Jianjun
authors.initials: J
authors.lastname: Li
authors.forename: Vera
authors.initials: V
authors.lastname: Lund
authors.forename: Eleonora
authors.initials: E
authors.lastname: Altman
mesh_terms: D002240:Carbohydrate Sequence; D053526:Listonella / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D019081:O Antigens / Q000737:chemistry* / Q000302:isolation & purification*
chemical_list: D019081:O Antigens