title: Characterization of glycoside hydrolase family 6 enzymes from Coprinopsis cinerea.
abstract: Recombinants, Cel6 from Coprinopsis cinerea (CcCel6)A, -B, and -C, were expressed by Escherichia coli. These enzymes produced cellobiose from phosphoric acid-swollen cellulose. When Avicel was used as the substrate, CcCel6A showed higher activity toward the substrate than CcCel6B or -C. In reaction with carboxymethylcellulose, CcCel6B and -C hydrolyzed the substrate, whereas CcCel6A failed to react.
authors.affiliation: Department of Environmental and Natural Resource Science, Faculty of Agriculture, Tokyo University of Agriculture and Technology, Fuchu, Tokyo, Japan.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Liu
authors.forename: Kiyohiko
authors.initials: K
authors.lastname: Igarashi
authors.forename: Satoshi
authors.initials: S
authors.lastname: Kaneko
authors.forename: Takashi
authors.initials: T
authors.lastname: Tonozuka
authors.forename: Masahiro
authors.initials: M
authors.lastname: Samejima
authors.forename: Kiyoharu
authors.initials: K
authors.lastname: Fukuda
authors.forename: Makoto
authors.initials: M
authors.lastname: Yoshida
mesh_terms: D001483:Base Sequence; D001487:Basidiomycota / Q000201:enzymology*; D002855:Chromatography, Thin Layer; D017931:DNA Primers; D004591:Electrophoresis, Polyacrylamide Gel; D006026:Glycoside Hydrolases / Q000378:metabolism*; D013379:Substrate Specificity
chemical_list: D017931:DNA Primers; D006026:Glycoside Hydrolases