title: Effect and mechanism of intense pulsed laser on skin aging in rats.
abstract: OBJECTIVE
  To explore the effect of intense pulsed laser (IPL) on anti-aging and its mechanism.
  
  
  METHODS
  Rat skin was continuously irradiated ex vivo by IPL at certain wavelengths with different energy densities. The rats were irradiated twice with an interval of 2 weeks. We measured superoxide dismutase (SOD) activity, malondialdehyde (MDA) content, hydroproline (Hyp) content, and the level of apoptosis-related gene B-cell lymphoma-2 (Bcl-2) and Bcl-2 associated X protein (Bax) in the IPL treatment groups, and then compared with those in the model groups and the control group.
  
  
  RESULTS
  SOD activity and Hyp content in the IPL treatment groups were higher than those in the model groups, and MDA content in the IPL treatment group was lower than that in the model group (P<0.05). IPL irradiation increased Bcl-2 protein content in rat skin cells, but had no effect on Bax protein expression.
  
  
  CONCLUSIONS
  IPL is effective to improve the appearance of aging skin. Its mechanism may relate to maintaining the balance of oxidation and anti-oxidation, restoring oxidase activity, and regulating the death of skin cells by increasing Bcl-2 expression.
authors.affiliation: Department of Dermatology, Third Xiangya Hospital, Central South University, Changsha 410013, China.
authors.forename: Xiang
authors.initials: X
authors.lastname: Yaping
authors.forename: Bin
authors.initials: B
authors.lastname: Wang
authors.forename: Jianyun
authors.initials: J
authors.lastname: Lu
authors.forename: Jing
authors.initials: J
authors.lastname: Chen
authors.forename: Chengxi
authors.initials: C
authors.lastname: Zuo
authors.forename: Lina
authors.initials: L
authors.lastname: Tan
authors.forename: Jinhua
authors.initials: J
authors.lastname: Huang
mesh_terms: D000818:Animals; D005260:Female; D007834:Lasers; D011392:Proline / Q000378:metabolism; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism*; D051381:Rats; D012867:Skin / Q000201:enzymology* / Q000378:metabolism; D015595:Skin Aging / Q000528:radiation effects*; D013482:Superoxide Dismutase / Q000378:metabolism*; D051028:bcl-2-Associated X Protein / Q000378:metabolism
chemical_list: C494828:Bax protein, rat; D019253:Proto-Oncogene Proteins c-bcl-2; D051028:bcl-2-Associated X Protein; D011392:Proline; D013482:Superoxide Dismutase