Dual optical coherence tomography/fluorescence microscopy for monitoring of Drosophila melanogaster larval heart.
This article demonstrates a combined instrument of two imaging modalities to acquire information on cardiac function in larval Drosophila melanogaster: optical coherence tomography (OCT) and laser scanning fluorescence microscopy (LSFM). For this purpose, a dedicated imaging instrument able to sequentially provide cross-sectional OCT and C-scan LSFM images has been developed. With this dual-imaging system, the heart can be easily located and visualized within the specimen and the change of the heart shape in a cardiac cycle can be monitored.
Bradu
Adrian
A
Applied Optics Group, School of Physical Sciences, University of Kent, CT2 7NH Canterbury, UK. a.bradu@kent.ac.uk
Ma
Lisha
L
Bloor
James W
JW
Podoleanu
Adrian
A
D000818:Animals; D004331:Drosophila melanogaster / Q000033:anatomy & histology / Q000254:growth & development / Q000502:physiology*; D005260:Female; D005583:Fourier Analysis; D005786:Gene Expression Regulation; D006321:Heart / Q000502:physiology*; D007814:Larva / Q000502:physiology; D008840:Microfilament Proteins / Q000378:metabolism; D018613:Microscopy, Confocal / Q000295:instrumentation / Q000379:methods*; D008856:Microscopy, Fluorescence / Q000295:instrumentation / Q000379:methods*; D021381:Protein Transport; D018511:Systems Integration; D041623:Tomography, Optical Coherence / Q000295:instrumentation / Q000379:methods*
D008840:Microfilament Proteins; C071058:moesin