title: Evaluation of new selective culture media and a rapid fluorescence in situ hybridization assay for identification of Clostridium difficile from stool samples.
abstract: Two new Clostridium difficile-selective agars, from Oxoid (according to Brazier) and from BD, were compared with cycloserine-cefoxitin-fructose agar (Oxoid) for their sensitivity of recovery of toxigenic C. difficile from stool samples. For the culture-positive samples, the sensitivities were 84.0, 42.6 and 90.4 %, respectively. In addition, a C. difficile-specific fluorescence in situ hybridization assay was developed, facilitating rapid and reliable identification of cultured isolates.
authors.affiliation: Institute of Medical Microbiology and Hygiene, University Hospital of Ulm, Ulm, Germany.
authors.forename: Kathrin
authors.initials: K
authors.lastname: Bloedt
authors.affiliation: Institute of Medical Microbiology and Hygiene, University Hospital of Ulm, Ulm, Germany.
authors.forename: Melanie
authors.initials: M
authors.lastname: Riecker
authors.affiliation: Department of Molecular Parasitology, Bernhard Nocht Institute for Tropical Medicine, Hamburg, Germany.
authors.forename: Sven
authors.initials: S
authors.lastname: Poppert
authors.affiliation: Dr Gaertner & Partner Laboratories, Ravensburg, Germany.|Institute of Medical Microbiology and Hygiene, University Hospital of Ulm, Ulm, Germany.
authors.forename: Nele
authors.initials: N
authors.lastname: Wellinghausen
mesh_terms: D000328:Adult; D002648:Child; D016360:Clostridioides difficile / Q000302:isolation & purification*; D003470:Culture Media / Q000737:chemistry*; D004761:Enterocolitis, Pseudomembranous / Q000175:diagnosis* / Q000382:microbiology*; D005243:Feces / Q000382:microbiology*; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*
chemical_list: D003470:Culture Media