title: [Evidence of Borrelia burgdorferi spirochetes in patients with early disseminated Lyme borreliosis].
abstract: Fifteen patients with clinically documented early disseminated lyme borreliosis were screened for the presence of spirochetes. In vitro trials of isolation of the pathogen were positive in 8 blood and 1 cerebrospinal fluid (CSF) samples. The positivity was further confirmed by electron microscopy, Western blot analysis and PCR. However, succesfull DNA extraction after using Borrelia specific primers proved the positivity in 9 blood and 1 CSF samples (67 %). Restriction fragment length polymorphism (RFLP) method using MseI restriction of PCR products of the amplified rrf-rrl region, allowed the identification of three species, namely the Borrelia garinii, B. afzelii and B. burgdorferi s.s.
authors.affiliation: Virologický Ustav, Slovenská Akadémia Vied, Bratislava. virukats@savba.sk
authors.forename: K
authors.initials: K
authors.lastname: Schwarzová
authors.forename: K
authors.initials: K
authors.lastname: Holecková
authors.forename: Z
authors.initials: Z
authors.lastname: Kostanová
mesh_terms: D000293:Adolescent; D000328:Adult; D025065:Borrelia burgdorferi / Q000302:isolation & purification*; D002648:Child; D004269:DNA, Bacterial / Q000097:blood / Q000134:cerebrospinal fluid; D005260:Female; D006801:Humans; D008193:Lyme Disease / Q000382:microbiology*; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length
chemical_list: D004269:DNA, Bacterial