Stability analysis of recombinant human TFF2 and its therapeutic effect on burn-induced gastric injury in mice.
The trefoil factor family 2 (TFF2), a member of the trefoil factor family, plays a critical role in maintaining homeostasis throughout the gastrointestinal tract. In the present study, we expressed recombinant human TFF2 by Escherichia coli expression system with the purity above 95%. In an in vitro simulated gastrointestinal environment, rhTFF2 was shown to exhibit resistance to proteases, heat, acid and alkali. In addition, in mouse burn-induced gastric injury models, rhTFF2 was demonstrated to accelerate the healing of gastric mucosal lesions. This study provides a new way to treat burn-induced gastric injury.
Sun
Yong
Y
Institute of Burn Research, Southwest Hospital, State Key Laboratory of Trauma, Burns and Combined Injury, The Third Military Medical University, Chongqing 40038, PR China. sunyong_97@hotmail.com
Wu
Wei
W
Zhang
Yong
Y
Lv
Shangjun
S
Wang
Lin
L
Wang
Shiliang
S
Peng
Xi
X
D000818:Animals; D002056:Burns / Q000188:drug therapy* / Q000473:pathology; D004353:Drug Evaluation, Preclinical; D004355:Drug Stability; D005753:Gastric Mucosa / Q000187:drug effects / Q000293:injuries* / Q000473:pathology; D006358:Hot Temperature; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008297:Male; D051379:Mice; D009336:Necrosis / Q000517:prevention & control; D010447:Peptide Hydrolases / Q000737:chemistry; D010455:Peptides / Q000737:chemistry / Q000627:therapeutic use*; D011994:Recombinant Proteins / Q000737:chemistry / Q000627:therapeutic use; D000071164:Trefoil Factor-2; D014945:Wound Healing / Q000187:drug effects
D010455:Peptides; D011994:Recombinant Proteins; C000604007:TFF2 protein, human; D000071164:Trefoil Factor-2; D010447:Peptide Hydrolases