Development of an antibody hapten-chip system for detecting the residues of multiple antibiotic drugs.
The abuse of antibiotic drugs during animal production remains a worldwide problem and the subsequent detection of the residues of various drugs present at low concentrations in complex biological matrices poses significant analytical challenges. The present study outlines a practical biochip assay system to identify antibiotic residues in different animal tissue extracts. The system uses a simple but efficient multiresidue sample extraction procedure to isolate the antibiotic residues which were then identified directly using high-affinity monoclonal antibodies presented in a competitive immunoassay with conjugated antibiotic hapten-chips. The hapten-chip can analyze six samples each for eight antibiotics on a single chip within 3 h. The analytical results with both artificial positive standard samples and the incurred samples show that the antibody hapten-chip system has a comparable accuracy and a similar sensitivity to a standard ultra performance liquid chromatography-mass spectrometry (MS)/MS assay. In conclusion, an effective analytical screening system based on antibody hapten-chip was developed for detecting multiple antibiotic residues from multiple samples.
Chen
Ailiang
A
Medical Systems Biology Research Center, Tsinghua University School of Medicine, Beijing 100084, China.
Wang
Guoqing
G
Cao
Qin
Q
Wang
Yan
Y
Zhang
Zhaohui
Z
Sun
Yimin
Y
Wang
Hui
H
Xu
Chaoyi
C
Zhou
Qi
Q
Han
Pei
P
Liu
Miao
M
Yang
Yang
Y
Xing
Wanli
W
Mitchelson
Keith R
KR
Cheng
Jing
J
D000818:Animals; D000900:Anti-Bacterial Agents / Q000032:analysis*; D000911:Antibodies, Monoclonal; D002645:Chickens; D002853:Chromatography, Liquid; D004350:Drug Residues; D006241:Haptens / Q000276:immunology*; D007118:Immunoassay / Q000379:methods*; D008099:Liver; D013058:Mass Spectrometry; D008461:Meat Products; D018482:Muscle, Skeletal; D013552:Swine; D046888:Tissue Array Analysis
D000900:Anti-Bacterial Agents; D000911:Antibodies, Monoclonal; D006241:Haptens