Potency fingerprint of herbal products Danshen injection for their quality evaluation.
The fingerprint technique has been studied frequently as a useful strategy for quality of traditional Chinese medicine. A novel potency fingerprint that can quantitatively analyze the antioxidant activity of individual constituent and provide the total antioxidant activities of the samples has been developed by high-performance liquid chromatography coupled with ultraviolet and pyrogallol-luminol chemiluminescence detection (HPLC-diode array detection (DAD)-PLD). Hierarchical clustering analysis has been used as a powerful pattern recognition tool to identify and classify Danshen injection from different factories. In addition, the combination use of the chromatographic fingerprint and potency fingerprint with principal component analysis was applied to quality control of Danshen injection. The results demonstrated that the proposed potency fingerprint was a useful means to control the quality and to clarify the possible mechanism of action of herbal products.
Chang
Yan-Xu
YX
The Key Laboratory for Prescription of National Educational Ministry of China, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China.
Yan
Dong-Mei
DM
Chen
Lin-Lin
LL
Ding
Xiao-Ping
XP
Qi
Jin
J
Kang
Li-Yuan
LY
Zhang
Bo-Li
BL
Yu
Bo-Yang
BY
D000975:Antioxidants / Q000737:chemistry* / Q000592:standards; D002851:Chromatography, High Pressure Liquid; D003627:Data Interpretation, Statistical; D004365:Drugs, Chinese Herbal / Q000737:chemistry* / Q000592:standards; D007202:Indicators and Reagents; D007267:Injections; D049449:Luminescence; D010634:Phenobarbital / Q000737:chemistry; D025341:Principal Component Analysis; D011748:Pyrogallol / Q000737:chemistry; D011786:Quality Control; D017382:Reactive Oxygen Species / Q000737:chemistry; D027561:Salvia miltiorrhiza / Q000737:chemistry*; D012996:Solutions; D013056:Spectrophotometry, Ultraviolet
D000975:Antioxidants; D004365:Drugs, Chinese Herbal; D007202:Indicators and Reagents; D017382:Reactive Oxygen Species; D012996:Solutions; D011748:Pyrogallol; D010634:Phenobarbital