title: Parallax: high accuracy three-dimensional single molecule tracking using split images.
abstract: Three-dimensional (3D) tracking can provide valuable biological insights that are missing in conventional microscopy. Here we developed a single molecule 3D tracking microscopy technique, named Parallax, with high localization precision and temporal resolution. We demonstrated its capabilities by studying the 3D trafficking of glucose-transporter-4 containing vesicles in living adipocytes as well as the walking path of single myosin VI molecules along actin filaments.
authors.affiliation: Pennsylvania Muscle Institute, University of Pennsylvania, 3700 Hamilton Walk, Philadelphia, Pennsylvania 19104, USA.
authors.forename: Yujie
authors.initials: Y
authors.lastname: Sun
authors.forename: Jennine Dawicki
authors.initials: JD
authors.lastname: McKenna
authors.forename: John M
authors.initials: JM
authors.lastname: Murray
authors.forename: E Michael
authors.initials: EM
authors.lastname: Ostap
authors.forename: Yale E
authors.initials: YE
authors.lastname: Goldman
mesh_terms: D017667:Adipocytes / Q000737:chemistry; D000818:Animals; D051275:Glucose Transporter Type 4 / Q000737:chemistry; D007090:Image Interpretation, Computer-Assisted / Q000379:methods; D021621:Imaging, Three-Dimensional / Q000379:methods*
chemical_list: D051275:Glucose Transporter Type 4