Arginine kinase peptide mass fingerprinting as a proteomic approach for species identification and taxonomic analysis of commercially relevant shrimp species.
A proteomic approach aimed at species identification and taxonomic analysis of shrimp species of commercial interest is presented. Six different species belonging to the order Decapoda were considered. Preliminary, two-dimensional gel electrophoresis (2-DE) analysis of the sarcoplasmic proteome revealed interspecific variability in the isoelectric point (pI) of arginine kinase. For this reason, arginine kinase spot was selected as a potential molecular marker and subjected to tryptic digestion followed by matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) peptide mass fingerprinting (PMF) analysis. Arginine kinase PMF allowed the differentiation of the six species studied. Four samples of commercial origin obtained in local markets were analyzed to validate the methodology. The PMF cluster analysis also provided information about the phylogenetic relationships in these species. The application of this methodology may be of interest for the differentiation and taxonomic analysis of shrimp species complementing DNA-based phylogenetic studies.
Ortea
Ignacio
I
Instituto de Investigaciones Marinas, Consejo Superior de Investigaciones Científicas, Vigo, Spain. nachoog@iim.csic.es
Cañas
Benito
B
Calo-Mata
Pilar
P
Barros-Velázquez
Jorge
J
Gallardo
José M
JM
D000818:Animals; D001122:Arginine Kinase / Q000737:chemistry*; D004247:DNA / Q000032:analysis; D033364:Decapoda / Q000145:classification* / Q000201:enzymology* / Q000235:genetics; D015180:Electrophoresis, Gel, Two-Dimensional; D007526:Isoelectric Point; D009132:Muscles / Q000737:chemistry; D010449:Peptide Mapping / Q000379:methods*; D010802:Phylogeny; D013045:Species Specificity; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014357:Trypsin / Q000378:metabolism
D004247:DNA; D001122:Arginine Kinase; D014357:Trypsin