title: Isoform-specific inhibition of cyclophilins.
abstract: Cyclophilins belong to the enzyme class of peptidyl prolyl cis-trans isomerases which catalyze the cis-trans isomerization of prolyl bonds in peptides and proteins in different folding states. Cyclophilins have been shown to be involved in a multitude of cellular functions like cell growth, proliferation, and motility. Among the 20 human cyclophilin isoenzymes, the two most abundant members of the cyclophilin family, CypA and CypB, exhibit specific cellular functions in several inflammatory diseases, cancer development, and HCV replication. A small-molecule inhibitor on the basis of aryl 1-indanylketones has now been shown to discriminate between CypA and CypB in vitro. CypA binding of this inhibitor has been characterized by fluorescence anisotropy- and isothermal titration calorimetry-based cyclosporin competition assays. Inhibition of CypA- but not CypB-mediated chemotaxis of mouse CD4(+) T cells by the inhibitor provided biological proof of discrimination in vivo.
authors.affiliation: Max Planck Research Unit for Enzymology of Protein Folding, Weinbergweg 22, 06120 Halle/Saale, Germany.
authors.forename: Sebastian
authors.initials: S
authors.lastname: Daum
authors.forename: Michael
authors.initials: M
authors.lastname: Schumann
authors.forename: Sebastian
authors.initials: S
authors.lastname: Mathea
authors.forename: Tobias
authors.initials: T
authors.lastname: Aumüller
authors.forename: Molly A
authors.initials: MA
authors.lastname: Balsley
authors.forename: Stephanie L
authors.initials: SL
authors.lastname: Constant
authors.forename: Boris Féaux
authors.initials: BF
authors.lastname: de Lacroix
authors.forename: Fabian
authors.initials: F
authors.lastname: Kruska
authors.forename: Manfred
authors.initials: M
authors.lastname: Braun
authors.forename: Cordelia
authors.initials: C
authors.lastname: Schiene-Fischer
mesh_terms: D000818:Animals; D001572:Benzofurans / Q000737:chemistry; D001667:Binding, Competitive; D055162:Biocatalysis; D015496:CD4-Positive T-Lymphocytes / Q000166:cytology; D020134:Catalytic Domain; D002633:Chemotaxis / Q000187:drug effects; D021984:Cyclophilin A / Q000037:antagonists & inhibitors / Q000737:chemistry / Q000378:metabolism; D021983:Cyclophilins / Q000037:antagonists & inhibitors* / Q000737:chemistry* / Q000378:metabolism; D016572:Cyclosporine / Q000737:chemistry / Q000378:metabolism; D004791:Enzyme Inhibitors / Q000737:chemistry* / Q000494:pharmacology*; D005454:Fluorescence Polarization; D006801:Humans; D007189:Indans / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D007527:Isoenzymes / Q000037:antagonists & inhibitors / Q000737:chemistry / Q000378:metabolism; D007700:Kinetics; D051379:Mice; D008815:Mice, Inbred Strains; D015394:Molecular Structure; D011485:Protein Binding; D011994:Recombinant Proteins / Q000037:antagonists & inhibitors / Q000737:chemistry / Q000378:metabolism; D013816:Thermodynamics
chemical_list: D001572:Benzofurans; D004791:Enzyme Inhibitors; D007189:Indans; D007527:Isoenzymes; D011994:Recombinant Proteins; C081786:cyclophilin B; D016572:Cyclosporine; D021984:Cyclophilin A; D021983:Cyclophilins