Relationships between single nucleotide polymorphisms of the H-FABP gene and slaughter and meat quality traits in chicken.
Using PCR-SSCP with five primer pairs, we detected six single nucleotide polymorphisms of the H-FABP gene: 332G --> A, 534G --> A, 783C --> T, 835C --> T, 1198T --> C, and 2329C --> T. Chi-square results showed significant differences (P < 0.05) in genotype frequency among breeds in Fragment 1 and extremely significant differences (P < 0.01) in Fragments 2-4. We found a significant association between Fragment 2 genotype and muscle fiber number, Arg and Thr (P < 0.05); between Fragment 3 genotype and living weight, carcass weight, breast muscle weight, abdominal fat weight, and abdominal fat percentage (P < 0.05); between Fragment 4 genotype and Thr, Phe, and inosinic acid (P < 0.05). It was concluded that H-FABP was the major gene influencing slaughter performance and meat quality or was linked with the major gene in these strains and that the C783T mutation could be used as a candidate molecular genetic marker for breeding selection. The combination M1C2-B2B2-D1D1 is an ideal model for breeding in these strains because it can improve slaughter and meat quality traits.
You
Xiaoyan
X
College of Animal Science and Technology, Sichuan Agriculture University, Ya'an, Sichuan, China.
Liu
Yiping
Y
Jiang
Xiaosong
X
Du
Huarui
H
Liu
Zuohua
Z
Zhu
Qing
Q
D000003:Abattoirs; D000818:Animals; D001483:Base Sequence; D001947:Breeding; D002645:Chickens / Q000033:anatomy & histology* / Q000145:classification / Q000235:genetics*; D050556:Fatty Acid-Binding Proteins / Q000235:genetics*; D005260:Female; D005524:Food Technology; D005787:Gene Frequency; D005838:Genotype; D008297:Male; D008460:Meat; D020641:Polymorphism, Single Nucleotide
D050556:Fatty Acid-Binding Proteins