title: Therapeutic silencing of an endogenous gene by siRNA cream in an arthritis model mouse.
abstract: Recent advances of biotechnology have laid the groundwork for potent and specific molecular-targeting therapies including RNA interference. The largest remaining hurdle for widespread use of this technology in skin is an effective delivery system. Here, we demonstrate an effective topical delivery system using a cream formulation containing a small-interfering RNA (siRNA) that specifically targets osteopontin (OPN). OPN is a validated target in numerous inflammatory diseases, including rheumatoid arthritis (RA). The siRNA targeting OPN was incorporated into a cream formulation GeneCream that penetrates the stratum corneum, depositing siRNA in the epidermis, dermis, and to a lesser extent, subcutaneous tissue. In addition, when the OPN siRNA cream was topically applied to the skin of a collagen antibody-induced RA mouse model, the siRNA cream prevented the occurrence of severe, irreversible damage to bone and cartilage. Thus, the siRNA cream provides effective delivery of active OPN siRNA, suggesting this formulation may represent a platform technology for delivery of siRNAs for treating various disorders including RA.
authors.affiliation: Department of Pathology, Tokyo Medical University, Tokyo, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Takanashi
authors.forename: K
authors.initials: K
authors.lastname: Oikawa
authors.forename: K
authors.initials: K
authors.lastname: Sudo
authors.forename: M
authors.initials: M
authors.lastname: Tanaka
authors.forename: K
authors.initials: K
authors.lastname: Fujita
authors.forename: A
authors.initials: A
authors.lastname: Ishikawa
authors.forename: S
authors.initials: S
authors.lastname: Nakae
authors.forename: R L
authors.initials: RL
authors.lastname: Kaspar
authors.forename: M
authors.initials: M
authors.lastname: Matsuzaki
authors.forename: M
authors.initials: M
authors.lastname: Kudo
authors.forename: M
authors.initials: M
authors.lastname: Kuroda
mesh_terms: D000287:Administration, Topical; D000818:Animals; D001169:Arthritis, Experimental / Q000628:therapy*; D001172:Arthritis, Rheumatoid / Q000628:therapy; D005260:Female; D005786:Gene Expression Regulation; D018390:Gene Targeting / Q000379:methods; D018014:Gene Transfer Techniques; D015316:Genetic Therapy / Q000379:methods*; D051379:Mice; D008807:Mice, Inbred BALB C; D009824:Ointments / Q000009:adverse effects; D053495:Osteopontin / Q000235:genetics* / Q000378:metabolism; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000008:administration & dosage; D012867:Skin; D012869:Skin Absorption
chemical_list: D009824:Ointments; D034741:RNA, Small Interfering; D053495:Osteopontin