Palmatine from Coptidis rhizoma reduces ischemia-reperfusion-mediated acute myocardial injury in the rat.
The aim of the present study was to evaluate the protective effect of palmatine, one of active ingredients of Coptidis rhizoma, against myocardial ischemia-reperfusion (I/R) injury is due to its antioxidant and anti-inflammatory action. Adult male rats were subjected to 30 min of ischemia and 6 or 24h of reperfusion. Rats were randomized to receive vehicle or palmatine 1h before reperfusion. Infarct size, myocardial function, and the antioxidant enzyme activity, such as malonaldehyde (MDA), lactate dehydrogenase (LDH), creatine phosphokinase (CK), superoxide dismutase (SOD) and catalase (CAT) were measured. Palmatine significantly improved I/R-induced myocardial dysfunction by increasing the values of the first derivative (+/-dp/dt) of left ventricular pressure and decreased infarct size by 50% (P<0.01 versus vehicle). As expected, palmatine markedly inhibited the increase of LDH, CK, and MDA contents in I/R rat serum, and it also significantly inhibited the decline of the activity of SOD and CAT in I/R cardiac tissues. In addition, COX-2 and iNOS expression in I/R myocardium was significantly reduced. Interestingly, palmatine increased heme oxygenase (HO)-1 induction in human aortic endothelial cells. We concluded that palmatine protects hearts from I/R injury in rats possibly by reducing oxidative stress and modulating inflammatory mediators.
Kim
Young Min
YM
Department of Pharmacology, School of Medicine, and Institute of Health Sciences, Gyeongsang National University, 92 Chilamdong, Jinju, Republic of Korea.
Ha
Yu Mi
YM
Jin
Yong Chun
YC
Shi
Lian Yu
LY
Lee
Yong Soo
YS
Kim
Hye Jung
HJ
Seo
Han Geuk
HG
Choi
Jae Soo
JS
Kim
Yeong Shik
YS
Kang
Sam Sik
SS
Lee
Jae Heun
JH
Chang
Ki Churl
KC
D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology; D000975:Antioxidants / Q000494:pharmacology; D001600:Berberine Alkaloids / Q000302:isolation & purification / Q000494:pharmacology*; D015153:Blotting, Western; D002316:Cardiotonic Agents; D000089042:Coptis chinensis; D003402:Creatine Kinase / Q000378:metabolism; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000494:pharmacology*; D006439:Hemodynamics / Q000187:drug effects; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D009203:Myocardial Infarction / Q000473:pathology; D015428:Myocardial Reperfusion Injury / Q000473:pathology / Q000517:prevention & control*; D009206:Myocardium / Q000473:pathology; D051381:Rats; D017207:Rats, Sprague-Dawley
D000894:Anti-Inflammatory Agents, Non-Steroidal; D000975:Antioxidants; D001600:Berberine Alkaloids; D002316:Cardiotonic Agents; D004365:Drugs, Chinese Herbal; D008315:Malondialdehyde; C107393:Coptidis rhizoma extract; D007770:L-Lactate Dehydrogenase; D003402:Creatine Kinase; C005413:palmatine