Reduction of nitro phenols using nitroreductase from E. coli in the presence of NADH.
The reductions of nitrophenols catalyzed by nitroreductase from E. coli in the presence of NADH were investigated in this paper. 4-Aminophenol and 4-hydroxylaminophenol were found in the reductive products of 4-nitrophenol and the maximum reductive ratio was about 83.49% when the reaction time was 70 min; 4,6-dinitro-2-pimelie kelone was found in the reductive products of 2,4-dinitrophenol and the maximum reductive ratio was about 75.28% when the reaction time was 80 min; 2,4-dinitrophenol and 4,6-dinitro-2-pimelie kelone were found in the reductive products of 2,4,6-trinitrophenol and the maximum reductive ratio was about 62.08% when the reaction time was 100 min. The similar reductive ratios of nitrophenols were obtained under aerobic and anaerobic conditions. The results indicated that nitroreductase was an oxygen-insensitive enzyme.
Dai
Rongji
R
School of Life Science and Technology, Beijing Institute of Technology, Beijing 100081, China. dairongji@bit.edu.cn
Chen
Jie
J
Lin
Jie
J
Xiao
Shenyuan
S
Chen
Shi
S
Deng
Yulin
Y
D019297:2,4-Dinitrophenol; D000627:Aminophenols; D001673:Biodegradation, Environmental; D004396:Coloring Agents; D029968:Escherichia coli Proteins; D015386:Hazardous Substances / Q000378:metabolism; D007700:Kinetics; D009153:Mutagens; D009243:NAD; D009596:Nitrophenols / Q000378:metabolism*; D009601:Nitroreductases / Q000378:metabolism*
D000627:Aminophenols; D004396:Coloring Agents; D029968:Escherichia coli Proteins; D015386:Hazardous Substances; D009153:Mutagens; D009596:Nitrophenols; D009243:NAD; D009601:Nitroreductases; D019297:2,4-Dinitrophenol; C026729:4-aminophenol