Influence of the microenvironment on gene and protein expression of odontogenic-like and osteogenic-like cells.
Progenitor cells play an important biological role in tooth and bone formation, and previous analyses during bone and dentine induction have indicated that they may be a good alternative for tissue engineering. Thus, to clarify the influence of the microenvironment on protein and gene expression, MDPC-23 cells (mouse dental papilla cell line) and KUSA/A1 cells (bone marrow stromal cell line) were used, both in vitro cell culture and in intra-abdominal diffusion chambers implanted in 4-week-old male immunodefficient mice (SCID mice). Our results indicate that KUSA/A1 cells differentiated into osteoblast-like cells and induced bone tissue inside the chamber, whereas, MDPC-23 showed odontoblast-like characteristics but with a low ability to induce dentin formation. This study shows that MDPC-23 cells are especial cells, which possess morphological and functional characteristics of odontoblast-like cells expressing dentin sialophosphoprotein in vivo. In contrast, dentin sialophosphoprotein gene and protein expression was not detected in both cell lines in vitro. The intra-abdominal diffusion chamber appears as an interesting experimental model for studying phenotypic expression of dental pulp cells in vivo.
Rodriguez
Andrea P
AP
Department of Oral Pathology and Medicine, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, 2-5-1 Shikata-Cho, Okayama 700-8558, Japan.
Tsujigiwa
Hidetsugu
H
Gunduz
Mehmet
M
Cengiz
Beyhan
B
Nagai
Noriyuki
N
Tamamura
Ryo
R
Borkosky
Silvia S
SS
Takagi
Tohru
T
Inoue
Miho
M
Nagatsuka
Hitoshi
H
D000818:Animals; D001861:Bone Regeneration / Q000235:genetics / Q000502:physiology*; D001842:Bone and Bones / Q000166:cytology / Q000378:metabolism; D002454:Cell Differentiation / Q000235:genetics / Q000502:physiology*; D002460:Cell Line; D024042:Collagen Type I / Q000096:biosynthesis / Q000235:genetics; D003771:Dental Papilla / Q000166:cytology / Q000378:metabolism; D015194:Diffusion Chambers, Culture / Q000379:methods; D015870:Gene Expression; D008297:Male; D051379:Mice; D016513:Mice, SCID; D009804:Odontoblasts / Q000166:cytology* / Q000378:metabolism; D009805:Odontogenesis / Q000235:genetics / Q000502:physiology*; D010006:Osteoblasts / Q000166:cytology* / Q000378:metabolism; D015675:Osteocalcin / Q000096:biosynthesis / Q000235:genetics; D015676:Osteonectin / Q000096:biosynthesis / Q000235:genetics; D053495:Osteopontin / Q000096:biosynthesis / Q000235:genetics; D014176:Protein Biosynthesis; D012795:Sialoglycoproteins / Q000096:biosynthesis / Q000235:genetics
D024042:Collagen Type I; D015676:Osteonectin; D012795:Sialoglycoproteins; C506896:Spp1 protein, mouse; D015675:Osteocalcin; D053495:Osteopontin