Interaction of olfactory ensheathing cells with nerve repairing scaffolds.
OBJECTIVE
To investigate a new way to yield plenty of high purity olfactory ensheathing cells (OECs) and its biocompatibility with appropriate scaffolds.


METHODS
OECs were prepared from neonatal Wister rats and co-cultured with poly [LA-co-(Glc-alt-Lys)] (PLGL). Its contact angle, adherent rate, and activity rate were tested.


RESULTS
The contact angle of poly (D, L-lactic acid) (PDLLA) (84.5 degree+/-1.5 degree) was significantly higher than that of PLGL (52.6 degree+/-0.8 degree), the adherent rate of PLGL (80%) was significantly higher than that of the PDLLA (57%), and the activity rate of PLGL (88%) was much higher than that of the PDLLA (76%).


CONCLUSIONS
PLGL possesses better hydrophilicity and biocompatibility than PDLLA, and it can provide a better cell growth circumstance which is helpful for the effective treatment of nerve injury.
Wang
Yonghong
Y
Department of Internal Medicine, Hospital of Wuhan University of Technology, Wuhan 430070, China. wangyonghong02@sina.com
Wang
Yonghong
Y
Yin
Yixia
Y
Li
Shipu
S
Yan
Qiongjiao
Q
Wan
Zhitao
Z
Han
Yingchao
Y
D000818:Animals; D000831:Animals, Newborn; D001672:Biocompatible Materials; D002478:Cells, Cultured; D019344:Lactic Acid / Q000138:chemical synthesis / Q000494:pharmacology*; D009416:Nerve Regeneration; D009830:Olfactory Bulb / Q000166:cytology*; D011100:Polyglycolic Acid / Q000138:chemical synthesis / Q000494:pharmacology*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D051381:Rats; D017208:Rats, Wistar; D013119:Spinal Cord Injuries / Q000503:physiopathology; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry*
D001672:Biocompatible Materials; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid