Antibacterial activity of recombinant human lactoferrin from rice: effect of heat treatment.
The antibacterial activity of recombinant human lactoferrin from rice (rhLF) compared with that of human milk lactoferrin (hLF) was evaluated against Escherichia coli O157:H7, Salmonella Enteritidis and Listeria monocytogenes. The hydrolysates of rhLF and hLF were found to be more active than native proteins against E. coli O157:H7, and their activity was independent of their iron saturation. The effect of different heat treatments on the antibacterial activity of apo-rhLF was studied and compared with hLF. We observed that an HTST pasteurization treatment did not affect the antimicrobial activity of lactoferrin against the pathogens studied. Furthermore, the activity of apo-rhLF and hLF against E. coli O157:H7 and L. monocytogenes in UHT milk and whey was assayed, finding a decrease in the number of bacteria, although lower than that observed in a broth medium. This study shows the similar antibacterial activity of rhLF and hLF which is important in order to consider the addition of rhLF as a supplement in special products.
Conesa
Celia
C
Department of Animal Production and Food Science, Food Technology Unit, Veterinary Faculty, University of Zaragoza, Zaragoza, Spain.
Rota
Carmen
C
Castillo
Eduardo
E
Pérez
María-Dolores
MD
Calvo
Miguel
M
Sánchez
Lourdes
L
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D003470:Culture Media; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000187:drug effects; D006358:Hot Temperature; D006801:Humans; D006868:Hydrolysis; D007781:Lactoferrin / Q000235:genetics / Q000494:pharmacology*; D008089:Listeria monocytogenes / Q000187:drug effects; D008826:Microbial Sensitivity Tests; D012275:Oryza / Q000235:genetics*; D011994:Recombinant Proteins / Q000235:genetics / Q000494:pharmacology; D012477:Salmonella enteritidis / Q000187:drug effects
D000900:Anti-Bacterial Agents; D003470:Culture Media; D011994:Recombinant Proteins; D007781:Lactoferrin