title: Anti-inflammatory effect of Columbianetin on activated human mast cells.
abstract: In the present study, we extracted Corydalis heterocarpa with various solvents in order to find the bioactive constituents that demonstrated anti-inflammatory effects. We isolated the active compound, Columbianetin. Anti-inflammatory effect of Columbianetin has been reported but the precise effects of Columbianetin in experimental models have remained unknown. In the present study, we investigate the effect of Columbianetin on the production of histamine, interleukin (IL)-1beta, IL-6, IL-8, and tumor necrosis factor (TNF)-alpha and expression of cyclooxygenase-2 (COX-2) by using the human mast cell line (HMC-1). Various concentrations of Columbianetin were treated before the activation of HMC-1 cells with phorbol 12-myristate 13-acetate (PMA) plus calcium ionophore, A23187. PMA plus A23187 significantly increased IL-1beta, IL-6, IL-8, and TNF-alpha production compared with media control (p<0.05). We also show that the increased cytokines IL-1beta, IL-6, IL-8, and TNF-alpha level was significantly inhibited by Columbianetin in a dose-dependent manner (p<0.05). Maximal inhibition rates of IL-1beta, IL-6, IL-8, and TNF-alpha production by Columbianetin were about 102.6%, 101.1%, 95.8%, and 103.9%, respectively. Columbianetin inhibited expression of COX-2. In addition, the effect of Columbianetin was investigated on the histamine release from HMC-1 stimulated by substance P, which promotes histamine release. Columbianetin also inhibited the histamine release by substance P. In conclusion, these results indicate that Columbianetin may be helpful in regulating mast cell-mediated allergic inflammatory responses.
authors.affiliation: Biochip Research Center, Hoseo University, Chungnam, Republic of Korea.
authors.forename: Hyun-Ja
authors.initials: HJ
authors.lastname: Jeong
authors.forename: Ho-Jeong
authors.initials: HJ
authors.lastname: Na
authors.forename: Su-Jin
authors.initials: SJ
authors.lastname: Kim
authors.forename: Hong-Kun
authors.initials: HK
authors.lastname: Rim
authors.forename: Noh-Yil
authors.initials: NY
authors.lastname: Myung
authors.forename: Phil-Dong
authors.initials: PD
authors.lastname: Moon
authors.forename: Na-Ra
authors.initials: NR
authors.lastname: Han
authors.forename: Jae-Uk
authors.initials: JU
authors.lastname: Seo
authors.forename: Tae-Hee
authors.initials: TH
authors.lastname: Kang
authors.forename: Jae-Joong
authors.initials: JJ
authors.lastname: Kim
authors.forename: Youngjin
authors.initials: Y
authors.lastname: Choi
authors.forename: In-Cheol
authors.initials: IC
authors.lastname: Kang
authors.forename: Seung-Heon
authors.initials: SH
authors.lastname: Hong
authors.forename: You-Ah
authors.initials: YA
authors.lastname: Kim
authors.forename: Young-Wan
authors.initials: YW
authors.lastname: Seo
authors.forename: Hyung-Min
authors.initials: HM
authors.lastname: Kim
authors.forename: Jae-Young
authors.initials: JY
authors.lastname: Um
mesh_terms: D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000302:isolation & purification / Q000494:pharmacology*; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D031311:Corydalis / Q000737:chemistry; D051546:Cyclooxygenase 2 / Q000096:biosynthesis; D011564:Furocoumarins / Q000302:isolation & purification / Q000494:pharmacology*; D006636:Histamine Release / Q000187:drug effects; D006801:Humans; D007378:Interleukins / Q000096:biosynthesis; D008407:Mast Cells / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D011564:Furocoumarins; D007378:Interleukins; D014409:Tumor Necrosis Factor-alpha; C046733:columbianetin; D051546:Cyclooxygenase 2