Effect of inducible expressed human cytomegalovirus immediate early 86 protein on cell apoptosis.
Human cytomegalovirus is a common human pathogen that can cause life-threatening disease under certain conditions. During infection of host cells, the virus expresses regulatory proteins such as IE72 and IE86 that are important for viral propagation. IE86 plays a critical role in the modulation of viral replication as well as host cell cycle control and apoptosis. In this study, a Tet-On system was used to quantify the effect of IE86 on apoptosis and p53 expression. Our results indicate that IE86 inhibits tumor necrosis factor (TNF)-alpha induced apoptosis and that the anti-apoptotic activity of this viral protein correlates with its expression levels. In addition, IE86 did not alter the mRNA level of p53. The system developed should provide a method for functional analysis of human cytomegalovirus (HCMV) IE86 protein.
Bai
Zhiqiang
Z
Department of Microbiology, Key Laboratory of Medicine and Biotechnology of Qingdao, Qingdao University Medical College, Qingdao, Shandong, China.
Li
Ling
L
Wang
Bin
B
Liu
Zhijun
Z
Wang
Haitao
H
Yan
Zhiyong
Z
Qian
Dongmeng
D
Ding
Shouyi
S
Song
Xuxia
X
D017209:Apoptosis / Q000502:physiology*; D001483:Base Sequence; D002453:Cell Cycle / Q000502:physiology; D002460:Cell Line; D017931:DNA Primers; D004318:Doxycycline / Q000494:pharmacology; D005434:Flow Cytometry; D015967:Gene Expression Regulation, Viral / Q000187:drug effects; D006801:Humans; D017874:Immediate-Early Proteins / Q000502:physiology*; D007150:Immunohistochemistry; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015534:Trans-Activators / Q000502:physiology*; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology
D017931:DNA Primers; C069186:IE2 protein, Cytomegalovirus; D017874:Immediate-Early Proteins; D015534:Trans-Activators; D014409:Tumor Necrosis Factor-alpha; D004318:Doxycycline