Enhancement of substrate concentration in microbial stereoinversion through one-pot oxidation and reduction by aqueous two-phase system.
An extractive biocatalytic method of aqueous two-phase system was employed for stereoinversing (R)-1-phenyl-1,2-ethanediol into (S)-1-phenyl-1,2-ethanediol by Candida parapsilosis CCTCC M203011. It was observed that substrate and product inhibitions in microbial stereoinversion through one-pot oxidation and reduction were removed efficiently by extractive biocatalysis in aqueous two-phase system with PEG 4000/phosphate potassium system, and that the substrate concentration was enhanced from 15 to 30 g/L with product optical purity of 99.02% e.e. and yield of 90% after 60 h. Simultaneously, it was observed that change in cell morphology impedes the further enhancement of substrate concentration in this system but can be reversibly changed after stereoinversion or cultivation in systems without PEG.
Xu
Min-wei
MW
Key Laboratory of Industrial Biotechnology, School of Biotechnology, Jiangnan University, Ministry of Education, 214122 Wuxi, People's Republic of China.
Mu
Xiao-qing
XQ
Xu
Yan
Y
D001709:Biotechnology / Q000379:methods*; D002021:Buffers; D002175:Candida / Q000201:enzymology*; D002384:Catalysis; D002625:Chemistry, Organic / Q000379:methods; D004305:Dose-Response Relationship, Drug; D010100:Oxygen / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry; D011108:Polymers / Q000737:chemistry; D011188:Potassium / Q000737:chemistry; D013237:Stereoisomerism; D013379:Substrate Specificity; D013997:Time Factors
D002021:Buffers; D011108:Polymers; D011092:Polyethylene Glycols; D011188:Potassium; D010100:Oxygen