title: Use of serology and polymerase chain reaction for the rapid eradication of small ruminant lentivirus infections from a sheep flock: a case report.
abstract: A SRLV-free sheep flock incurred infection which led to an SRLV infection rate of over 50% of the ewes (34/64) within a 30 months period, indicating that environmental conditions were favourable to transmission. An intensive regimen of sampling at short intervals and testing for SRLV antibodies and proviral DNA combined with strict management was implemented for the entire flock, lambs and yearlings included. This resulted in eradication of the infection within two testing and culling rounds with a 3 months interval. The additional value of the proviral DNA detection by PCR in identifying infected animals was clear in that nine infected animals were found that would have been missed if tested by serology alone. PCR also saved two lambs from being culled; they were sero-positive probably due to maternal antibodies, but not infected.
authors.affiliation: Department for Diagnostics, Research and Epidemiology, Animal Health Service Deventer, 7400 AA Deventer, The Netherlands. j.brinkhof@gddeventer.com
authors.forename: J M A
authors.initials: JM
authors.lastname: Brinkhof
authors.forename: L
authors.initials: L
authors.lastname: Moll
authors.forename: C
authors.initials: C
authors.lastname: van Maanen
authors.forename: D J
authors.initials: DJ
authors.lastname: Houwers
mesh_terms: D000367:Age Factors; D000818:Animals; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005260:Female; D016180:Lentivirus Infections / Q000175:diagnosis / Q000517:prevention & control / Q000662:veterinary*; D017972:Lentiviruses, Ovine-Caprine; D016133:Polymerase Chain Reaction / Q000662:veterinary; D012756:Sheep / Q000097:blood / Q000821:virology; D012757:Sheep Diseases / Q000097:blood / Q000175:diagnosis / Q000517:prevention & control / Q000821:virology*