title: Arginine kinase peptide mass fingerprinting as a proteomic approach for species identification and taxonomic analysis of commercially relevant shrimp species.
abstract: A proteomic approach aimed at species identification and taxonomic analysis of shrimp species of commercial interest is presented. Six different species belonging to the order Decapoda were considered. Preliminary, two-dimensional gel electrophoresis (2-DE) analysis of the sarcoplasmic proteome revealed interspecific variability in the isoelectric point (pI) of arginine kinase. For this reason, arginine kinase spot was selected as a potential molecular marker and subjected to tryptic digestion followed by matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) peptide mass fingerprinting (PMF) analysis. Arginine kinase PMF allowed the differentiation of the six species studied. Four samples of commercial origin obtained in local markets were analyzed to validate the methodology. The PMF cluster analysis also provided information about the phylogenetic relationships in these species. The application of this methodology may be of interest for the differentiation and taxonomic analysis of shrimp species complementing DNA-based phylogenetic studies.
authors.affiliation: Instituto de Investigaciones Marinas, Consejo Superior de Investigaciones Científicas, Vigo, Spain. nachoog@iim.csic.es
authors.forename: Ignacio
authors.initials: I
authors.lastname: Ortea
authors.forename: Benito
authors.initials: B
authors.lastname: Cañas
authors.forename: Pilar
authors.initials: P
authors.lastname: Calo-Mata
authors.forename: Jorge
authors.initials: J
authors.lastname: Barros-Velázquez
authors.forename: José M
authors.initials: JM
authors.lastname: Gallardo
mesh_terms: D000818:Animals; D001122:Arginine Kinase / Q000737:chemistry*; D004247:DNA / Q000032:analysis; D033364:Decapoda / Q000145:classification* / Q000201:enzymology* / Q000235:genetics; D015180:Electrophoresis, Gel, Two-Dimensional; D007526:Isoelectric Point; D009132:Muscles / Q000737:chemistry; D010449:Peptide Mapping / Q000379:methods*; D010802:Phylogeny; D013045:Species Specificity; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014357:Trypsin / Q000378:metabolism
chemical_list: D004247:DNA; D001122:Arginine Kinase; D014357:Trypsin