Simultaneous determination of phenolic acids and phthalide compounds by liquid chromatography for quality assessment of Rhizoma cnidii.
This paper describes a simple, rapid, and validated reversed-phase high-performance liquid chromatographic method developed for the determination of 4 major bioactive constituents, namely, chlorogenic acid, ferulic acid, senkyunolide A, and Z-ligustilide in Rhizoma Cnidii extract. A Capcell Pak C18 chromatographic column (150 x 4.6 mm, 3 microm) was used with mobile phases consisting of 0.1% formic acid, acetonitrile, and methanol at a flow rate of 0.8 mL/min and UV detection at 285 nm. Comprehensive validation of the method included evaluation of linearity, repeatability, recovery, and stability. Excellent linear behavior (r2>0.99) was observed over the concentration range of 2-100 microg/mL for the compounds under investigation. Repeatability and accuracy were evaluated by intra- and interday assays; the relative standard deviation (RSD) values were < or = 5.37% and accuracies ranged from 97.1 to 104.9%. Recoveries of the compounds ranged from 94.2 to 104.2% with RSD values of < or = 9.50%. The developed method was successfully applied to the analysis of ethanolic extracts of Rhizoma Cnidii samples. As a result, the concentrations of chlorogenic acid, ferulic acid, Z-ligustilide, and senkyunolide A were determined to be 0.84-5.35, 0.45-1.65, 0.74-4.39, and 0.32-1.14 mg/g herb, respectively. Thus, the developed method was found to be accurate and reproducible and is considered suitable for the qualitative and quantitative analysis of Rhizoma Cnidii for bioactive compounds.
Islam
M Nurul
MN
Korea Institute of Science and Technology, Doping Control Center, PO Box 131, Chungryang, Seoul 130-650, Korea.
Yoo
Hye Hyun
HH
Sung
Chung-Ki
CK
Dong
Mi-sook
MS
Park
Young In
YI
Jin
Changbae
C
Kim
Dong-Hyun
DH
D015107:4-Butyrolactone / Q000031:analogs & derivatives / Q000032:analysis; D001572:Benzofurans / Q000032:analysis* / Q000592:standards; D002726:Chlorogenic Acid / Q000032:analysis; D002851:Chromatography, High Pressure Liquid / Q000379:methods* / Q000592:standards; D031048:Cnidium / Q000737:chemistry*; D003373:Coumaric Acids / Q000032:analysis; D062385:Hydroxybenzoates / Q000032:analysis* / Q000592:standards; D007723:Korea; D055670:Medicine, Korean Traditional; D010936:Plant Extracts / Q000032:analysis / Q000592:standards; D011786:Quality Control; D015203:Reproducibility of Results; D021241:Spectrometry, Mass, Electrospray Ionization
D001572:Benzofurans; D003373:Coumaric Acids; D062385:Hydroxybenzoates; D010936:Plant Extracts; D002726:Chlorogenic Acid; C027820:ligustilide; C045855:3-N-butyl-4,5-dihydrophthalide; C082022:phthalide; C004999:ferulic acid; C017616:phenolic acid; D015107:4-Butyrolactone