[Effects of inhibition of ANG-2 expression in Ishikawa cell line].
OBJECTIVE
To investigate the effect of RNA interference mediated angiopoietin-2 (ANG-2) gene silencing on human endometrial carcinoma cell line Ishikawa.


METHODS
Short hairpin RNA (shRNA) targeting ANG-2 gene was designed and transfected into Ishikawa cells with Lipofectamine 2000. The mRNA and protein expression level of ANG-2, proliferation, morphological changes, apoptosis, cell cycle and invasive ability of the cells after transfection were analyzed.


RESULTS
The shRNA targeting the human ANG-2 gene effectively decreased the expression of ANG-2 on both mRNA and protein level, the proliferation inhibition rate of the Ishikawa cells was 63.11%, cell apoptosis was induced, and the cell cycle was arrested in G1 phase. The apoptotic rate of the Ishikawa cells in the transfected group was significantly higher, and the invasive ability was decreased markedly, than that of control groups.


CONCLUSIONS
The shRNA targeting human ANG-2 gene could reduce ANG-2 expression, inhibit cellular growth and invasion in Ishikawa cells in vitro.
Zhou
Huai-jun
HJ
Department of Obstetrics and Gynecology, the Affiliated Drum Tower Hospital, Nanjing University School of Medicine, Nanjing, Jiangsu, 210008 PR China.
He
Rong-rong
RR
Xia
Bao-mei
BM
Xun
Qing-ying
QY
Pan
Jin-shun
JS
Hu
Ya-li
YL
D042702:Angiopoietin-2 / Q000037:antagonists & inhibitors / Q000235:genetics*; D017209:Apoptosis / Q000187:drug effects / Q000235:genetics*; D002453:Cell Cycle / Q000187:drug effects / Q000235:genetics; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D016889:Endometrial Neoplasms / Q000235:genetics* / Q000473:pathology; D005260:Female; D015870:Gene Expression / Q000187:drug effects; D015972:Gene Expression Regulation, Neoplastic; D020868:Gene Silencing / Q000187:drug effects* / Q000502:physiology; D006801:Humans; D007202:Indicators and Reagents; D008055:Lipids / Q000494:pharmacology*; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics / Q000494:pharmacology*; D014162:Transfection; D014407:Tumor Cells, Cultured
D042702:Angiopoietin-2; D007202:Indicators and Reagents; D008055:Lipids; C086724:Lipofectamine; D034741:RNA, Small Interfering