title: Long term exposure to the chemokine CCL2 activates the nigrostriatal dopamine system: a novel mechanism for the control of dopamine release.
abstract: Accumulating evidence show that chemokines can modulate the activity of neurons through various mechanisms. Recently, we demonstrated that CCR2, the main receptor for the chemokine CCL2, is constitutively expressed in dopamine neurons in the rat substantia nigra. Here we show that unilateral intranigral injections of CCL2 (50 ng) in freely moving rats increase extracellular concentrations of dopamine and its metabolites and decrease dopamine content in the ipsilateral dorsal striatum. Furthermore, these CCL2 injections are responsible for an increase in locomotor activity resulting in contralateral circling behavior. Using patch-clamp recordings of dopaminergic neurons in slices of the rat substantia nigra, we observed that a prolonged exposure (>8 min) to 10 nM CCL2 significantly increases the membrane resistance of dopaminergic neurons by closure of background channels mainly selective to potassium ions. This leads to an enhancement of dopaminergic neuron discharge in pacemaker or burst mode necessary for dopamine release. We provide here the first evidence that application of CCL2 on dopaminergic neurons increases their excitability, dopamine release and related locomotor activity.
authors.affiliation: Institut de Pharmacologie Moléculaire et Cellulaire, CNRS UNSA, UMR, 6097 CNRS, 660 Route des Lucioles, Sophia Antipolis, 06560, Valbonne, France. alice.guyon@ipmc.cnrs.fr
authors.forename: A
authors.initials: A
authors.lastname: Guyon
authors.forename: D
authors.initials: D
authors.lastname: Skrzydelski
authors.forename: I
authors.initials: I
authors.lastname: De Giry
authors.forename: C
authors.initials: C
authors.lastname: Rovère
authors.forename: G
authors.initials: G
authors.lastname: Conductier
authors.forename: J M
authors.initials: JM
authors.lastname: Trocello
authors.forename: V
authors.initials: V
authors.lastname: Daugé
authors.forename: P
authors.initials: P
authors.lastname: Kitabgi
authors.forename: W
authors.initials: W
authors.lastname: Rostène
authors.forename: J L
authors.initials: JL
authors.lastname: Nahon
authors.forename: S
authors.initials: S
authors.lastname: Mélik Parsadaniantz
mesh_terms: D000818:Animals; D002462:Cell Membrane / Q000502:physiology; D018932:Chemokine CCL2 / Q000494:pharmacology / Q000502:physiology*; D003342:Corpus Striatum / Q000187:drug effects / Q000378:metabolism*; D004298:Dopamine / Q000378:metabolism*; D066298:In Vitro Techniques; D015640:Ion Channel Gating; D008297:Male; D017551:Microdialysis; D009043:Motor Activity / Q000187:drug effects; D009474:Neurons / Q000187:drug effects / Q000502:physiology; D018408:Patch-Clamp Techniques; D015221:Potassium Channels / Q000502:physiology; D051381:Rats; D017208:Rats, Wistar; D013239:Stereotyped Behavior / Q000187:drug effects; D013378:Substantia Nigra / Q000187:drug effects / Q000378:metabolism*; D013997:Time Factors
chemical_list: C491766:Ccl2 protein, rat; D018932:Chemokine CCL2; D015221:Potassium Channels; D004298:Dopamine