Identification of the major proteins produced by cultured germline stem cells in chicken.
Although chicken spermatogonial stem cells (SCs) are important in spermatogenesis and transgenic research, little is known about these cells. Recently, our group constructed an in vitro culture system to establish germline stem cells (GSCs). To examine the mechanism of chicken spermatogonial SC development, we constructed a proteome map of GSCs from 4-week-old chicken testes. Soluble extracts of the GSCs were fractionated by 2-dimensional gel electrophoresis (pH 4-7). Several protein spots, including those that displayed significantly high levels, were identified by matrix-assisted laser desorption/ionization-time of flight mass spectrometry and liquid chromatography-tandem mass spectrometry. Of the 82/250 GSC spots examined, 56 yielded mass spectra that matched avian proteins found in the on-line databases. All of the identified proteins were classified into functional groups. This type of proteome map is an important resource for research on spermatogenesis and transgenesis.
Han
Beom Ku
BK
Avicore Biotechnology Institute, Optifarm Solution Inc, Geumjeong-Dong, Gyeonggi-Do, Korea.
Jung
Jin Gyoung
JG
Nam
Jungrye
J
Moon
Jong Kook
JK
Kim
Jin Nam
JN
Lee
Sang In
SI
Kim
Jin Kyoo
JK
Kim
Heebal
H
Han
Jae Yong
JY
D000818:Animals; D002478:Cells, Cultured; D002645:Chickens; D020869:Gene Expression Profiling; D008297:Male; D020543:Proteome; D013093:Spermatogonia / Q000378:metabolism*
D020543:Proteome