Ellagitannins from Terminalia calamansanai induced apoptosis in HL-60 cells.
Terminalia calamansanai (Blanco) Rolf. (Combretaceae) is used medicinally as lithontriptic in Philippines. The 70% acetone extracts of T. calamansanai leaves inhibited the viability of human promyelocytic leukemia HL-60 cells. 1-Alpha-O-galloylpunicalagin, punicalagin, 2-O-galloylpunicalin, sanguiin H-4, and methyl gallate were the main components isolated from T. calamansanai with the IC(50) values of 65.2, 74.8, 42.2, 38.0 and >100 microM, respectively, for HL-60 cells. Apoptosis of HL-60 cells treated with 1-Alpha-O-galloylpunicalagin, punicalagin, 2-O-galloylpunicalin, and sanguiin H-4 was noted by the appearance of a sub-G(1) peak in flow cytometric analysis and DNA fragmentation by gel electrophoresis. 2-O-Galloylpunicalin and sanguiin H-4 induced a decrease of the human poly(ADP-ribose)polymerase (PARP) cleavage-related procaspase-3 and elevated activity of caspase-3 in HL-60 cells, but not normal human peripheral blood mononuclear cells (PBMCs), suggesting that both compounds may be new candidates for drug development in the prevention and treatment of cancer.
Chen
Lih-Geeng
LG
Graduate Institute of Biomedical and Biopharmaceutical Sciences, College of Life Sciences, National Chiayi University, Chiayi 600, Taiwan, ROC.
Huang
Wen-Tsung
WT
Lee
Lain-Tze
LT
Wang
Ching-Chiung
CC
D017209:Apoptosis / Q000187:drug effects*; D053148:Caspase 3 / Q000502:physiology; D002470:Cell Survival / Q000187:drug effects; D018922:HL-60 Cells; D006801:Humans; D047348:Hydrolyzable Tannins / Q000494:pharmacology*; D029766:Terminalia / Q000737:chemistry*
D047348:Hydrolyzable Tannins; D053148:Caspase 3