High specificity of quantitative methylation-specific PCR analysis for MGMT promoter hypermethylation detection in gliomas.
Normal brain tissue from 28 individuals and 50 glioma samples were analyzed by real-time Quantitative Methylation-Specific PCR (QMSP). Data from this analysis were compared with results obtained on the same samples by MSP. QMSP analysis demonstrated a statistically significant difference in both methylation level (P = .000009 Mann Whitney Test) and frequencies (P = .0000007, Z-test) in tumour samples as compared with normal brain tissues. Although QMSP and MSP showed similar sensitivity, the specificity of QMSP analysis was significantly higher (93%; CI95%: 84%-100%) as compared with MSP (64%; 95%CI: 46%-82%). Our results suggest that QMSP analysis may represent a powerful tool to identify glioma patients that will benefit from alkylating agents chemotherapy.
Parrella
Paola
P
Oncology Laboratory, IRCCS Casa Sollievo della Sofferenza Hospital, 71013 San Giovanni Rotondo, Italy. pparrella@operapadrepio.it
la Torre
Antonella
A
Copetti
Massimiliano
M
Valori
Vanna M
VM
Barbano
Raffaela
R
Notarangelo
Angelo
A
Bisceglia
Michele
M
Gallo
Antonietta Pia
AP
Balsamo
Teresa
T
Poeta
Maria Luana
ML
Carella
Massimo
M
Catapano
Domenico
D
Parisi
Salvatore
S
Dallapiccola
Bruno
B
Maiello
Evaristo
E
D'Angelo
Vincenzo
V
Fazio
Vito Michele
VM
D019540:Area Under Curve; D001921:Brain / Q000378:metabolism; D001923:Brain Chemistry; D019175:DNA Methylation; D015254:DNA Modification Methylases / Q000235:genetics*; D045643:DNA Repair Enzymes / Q000235:genetics*; D005260:Female; D005910:Glioma / Q000235:genetics*; D006801:Humans; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction / Q000379:methods*; D011401:Promoter Regions, Genetic; D012372:ROC Curve; D012680:Sensitivity and Specificity; D018709:Statistics, Nonparametric; D025521:Tumor Suppressor Proteins / Q000235:genetics*
D025521:Tumor Suppressor Proteins; D015254:DNA Modification Methylases; C515491:MGMT protein, human; D045643:DNA Repair Enzymes