title: Risk of equine infectious anemia virus disease transmission through in vitro embryo production using somatic cell nuclear transfer.
abstract: Prevention and regulation of equine infectious anemia virus (EIAV) disease transmission solely depend on identification, isolation, and elimination of infected animals because of lack of an effective vaccine. Embryo production via the somatic cell nuclear transfer (SCNT) technology uses oocytes collected mainly from untested animals, which creates a potential risk of EIAV transmission through infected embryos. The current review examines the risk of EIAV disease transmission through SCNT embryo production and transfer. Equine infectious anemia virus is a lentivirus from the family Retroviridae. Because of a lack of direct reports on this subject, relevant information gathered from close relatives of EIAV, such as human immunodeficiency virus (HIV), bovine immunodeficiency virus (BIV), feline immunodeficiency virus (FIV), and small ruminant lentiviruses (SRLVs), is summarized and used to predict the biological plausibility of EIAV disease transmission through transfers of the equine SCNT embryos. Based on published information regarding interaction of oocytes with lentiviruses and the sufficiency of oocyte and embryo washing procedures to prevent lentivirus transmission from in vitro-produced embryos of various species, we predicted the risk of EIAV transmission through SCNT embryo production and transfer to be very small or absent.
authors.affiliation: Viagen, Inc., 12357-A Riata Trace Parkway, Suite 100, Austin, TX 78727, USA. Keqin.gregg@viagen.com
authors.forename: K
authors.initials: K
authors.lastname: Gregg
authors.forename: I
authors.initials: I
authors.lastname: Polejaeva
mesh_terms: D000818:Animals; D002415:Cats; D002417:Cattle; D019976:Cloning, Organism / Q000009:adverse effects / Q000379:methods*; D004624:Embryo Transfer / Q000662:veterinary; D004622:Embryo, Mammalian / Q000821:virology; D004859:Equine Infectious Anemia / Q000635:transmission*; D006736:Horses; D006801:Humans; D004860:Infectious Anemia Virus, Equine / Q000502:physiology*; D053652:Nuclear Transfer Techniques / Q000009:adverse effects / Q000662:veterinary*; D012307:Risk Factors