title: [Use of the real time RT-PCR for immune related gene expression quantitation in experimentally infected Neospora caninum bovine calves].
abstract: Neospora caninum is one of the main causes of abortion and natimortality in cattle. Host immune defense is capable to inhibit tachyzoite activity during acute infection, but there is no action against bradyzoites in tissue cysts. Activation and modulation of this response is controlled by cell mediators. The real-time RT-PCR technique was employed to detect some of those mediators during N. caninum infection. Holstein and Nelore calves intramuscularly infected with tachyzoites and uninfected controls were slaughtered at the sixth day post-infection and popliteal lymph node, liver and brain cortex samples were analyzed. Real-time RT-PCR detected gene expression in all tissues. No significant variation of GAPDH gene expression was detected among groups, its amplification efficiency was similar to the other genes tested and it was used as the endogenous control for the analysis. Comparisons between infected and uninfected groups allowed the relative gene expression quantification. IFN-gamma and TNF-alpha genes showed increased expression in some samples. iNOS and TGF-beta1 genes had some non-significant variations and IL-4 and IL-10 stayed practically unaltered.
authors.affiliation: Departamento de Medicina Veterinária Preventiva e Saúde Animal, Faculdade de Medicina Veterinária e Zootecnia--FMVZ, Universidade de São Paulo--USP, Brazil. smnishi@usp.br.
authors.forename: Sandra Mayumi
authors.initials: SM
authors.lastname: Nishi
authors.forename: Luciana Mori
authors.initials: LM
authors.lastname: Viero
authors.forename: Rodrigo Martins
authors.initials: RM
authors.lastname: Soares
authors.forename: Paulo Cesar
authors.initials: PC
authors.lastname: Maiorka
authors.forename: Solange Maria
authors.initials: SM
authors.lastname: Gennari
mesh_terms: D000818:Animals; D002417:Cattle; D003048:Coccidiosis / Q000235:genetics* / Q000276:immunology*; D015870:Gene Expression / Q000276:immunology*; D018688:Neospora; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013997:Time Factors