Rapid qualitative and quantitative analysis of opiates in extract of poppy head via FTIR and chemometrics: towards in-field sensors.
Identification and quantification of the opiates morphine and thebaine has been achieved in three commercial poppy cultivars using FTIR-ATR spectroscopy, from a simple and rapid methanolic extraction, suitable for field analysis. The limits of detection were 0.13 mg/ml (0.013%, w/v) and 0.3 mg/ml (0.03%, w/v) respectively. The concentrations of opiates present were verified with HPLC-MS. The chemometrics has been used to identify specific "signature" peaks in the poppy IR spectra for characterisation of cultivar by its unique fingerprint offering a potential forensic application in opiate crop analysis.
Turner
Nicholas W
NW
Cranfield University, Cranfield, Bedfordshire, UK. nicholas.turner@newcastle.edu.au
Cauchi
Michael
M
Piletska
Elena V
EV
Preston
Christopher
C
Piletsky
Sergey A
SA
D000465:Algorithms; D000701:Analgesics, Opioid / Q000032:analysis*; D015374:Biosensing Techniques / Q000379:methods*; D020650:Combinatorial Chemistry Techniques / Q000379:methods*; D010207:Papaver / Q000737:chemistry*; D010936:Plant Extracts / Q000032:analysis*; D012680:Sensitivity and Specificity; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods*
D000701:Analgesics, Opioid; D010936:Plant Extracts