Involvement of afadin in the formation and remodeling of synapses in the hippocampus.
In the hippocampus, synapses are formed between mossy fiber terminals and CA3 pyramidal cell dendrites and comprise highly developed synaptic junctions (SJs) and puncta adherentia junctions (PAJs). Dynamic remodeling of synapses in the hippocampus is implicated in learning and memory. Components of both the nectin-afadin and cadherin-catenin cell adhesion systems exclusively accumulate at PAJs. We investigated the role of afadin at synapses in mice in which the afadin gene was conditionally inactivated in hippocampal neurons. In these mutant mice, the signals for not only nectins, but also N-cadherin and beta-catenin, were hardly detected in the CA3 area, in addition to loss of the signal for afadin, resulting in disruption of PAJs. Ultrastructural analysis revealed an increase in the number of perforated synapses, suggesting the instability of SJs. These results indicate that afadin is involved not only in the assembly of nectins and cadherins at synapses, but also in synaptic remodeling.
Majima
Takashi
T
Department of Biochemistry, Osaka University Graduate School of Medicine, Japan.
Ogita
Hisakazu
H
Yamada
Tomohiro
T
Amano
Hisayuki
H
Togashi
Hideru
H
Sakisaka
Toshiaki
T
Tanaka-Okamoto
Miki
M
Ishizaki
Hiroyoshi
H
Miyoshi
Jun
J
Takai
Yoshimi
Y
D000818:Animals; D015820:Cadherins / Q000378:metabolism; D015815:Cell Adhesion Molecules / Q000378:metabolism; D006624:Hippocampus / Q000166:cytology / Q000201:enzymology*; D011495:Histone-Lysine N-Methyltransferase / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D018345:Mice, Knockout; D008840:Microfilament Proteins / Q000235:genetics / Q000378:metabolism*; D000075983:Nectins; D017966:Pyramidal Cells / Q000201:enzymology / Q000648:ultrastructure; D013569:Synapses / Q000201:enzymology*; D051176:beta Catenin / Q000378:metabolism
D015820:Cadherins; D015815:Cell Adhesion Molecules; D008840:Microfilament Proteins; D000075983:Nectins; C108835:afadin; D051176:beta Catenin; D011495:Histone-Lysine N-Methyltransferase; C516439:MLL4 protein, mouse