Ghrelin O-acyltransferase (GOAT) has a preference for n-hexanoyl-CoA over n-octanoyl-CoA as an acyl donor.
Ghrelin is a peptide hormone in which serine 3 is modified by n-octanoic acid through GOAT (ghrelin O-acyltransferase). However, the enzymological properties of GOAT remain to be elucidated. We analyzed the in vitro activity of GOAT using the recombinant enzyme. Unexpectedly, although the main active form of ghrelin is modified by n-octanoic acid, GOAT had a strong preference for n-hexanoyl-CoA over n-octanoyl-CoA as an acyl donor. Moreover, a four-amino acid peptide derived from the N-terminal sequence of ghrelin can be modified by GOAT, indicating that these four amino acids constitute the core motif for substrate recognition by the enzyme.
Ohgusu
Hideko
H
Molecular Genetics, Institute of Life Science, Kurume University, Hyakunenkohen 1-1, Kurume, Fukuoka 839-0864, Japan.
Shirouzu
Kaori
K
Nakamura
Yuki
Y
Nakashima
Yoshiki
Y
Ida
Takanori
T
Sato
Takahiro
T
Kojima
Masayasu
M
D000214:Acyl Coenzyme A / Q000737:chemistry*; D000217:Acyltransferases / Q000737:chemistry*; D000595:Amino Acid Sequence; D000818:Animals; D054439:Ghrelin / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D008565:Membrane Proteins; D051379:Mice; D010455:Peptides / Q000737:chemistry; D013379:Substrate Specificity; D013696:Temperature
D000214:Acyl Coenzyme A; D054439:Ghrelin; D008565:Membrane Proteins; D010455:Peptides; C034681:octanoyl-coenzyme A; C044181:hexanoyl-coenzyme A; D000217:Acyltransferases; C526983:Mboat4 protein, mouse