title: Characterization of airborne microbial communities at a high-elevation site and their potential to act as atmospheric ice nuclei.
abstract: Bacteria and fungi are ubiquitous in the atmosphere. The diversity and abundance of airborne microbes may be strongly influenced by atmospheric conditions or even influence atmospheric conditions themselves by acting as ice nucleators. However, few comprehensive studies have described the diversity and dynamics of airborne bacteria and fungi based on culture-independent techniques. We document atmospheric microbial abundance, community composition, and ice nucleation at a high-elevation site in northwestern Colorado. We used a standard small-subunit rRNA gene Sanger sequencing approach for total microbial community analysis and a bacteria-specific 16S rRNA bar-coded pyrosequencing approach (4,864 sequences total). During the 2-week collection period, total microbial abundances were relatively constant, ranging from 9.6 x 10(5) to 6.6 x 10(6) cells m(-3) of air, and the diversity and composition of the airborne microbial communities were also relatively static. Bacteria and fungi were nearly equivalent, and members of the proteobacterial groups Burkholderiales and Moraxellaceae (particularly the genus Psychrobacter) were dominant. These taxa were not always the most abundant in freshly fallen snow samples collected at this site. Although there was minimal variability in microbial abundances and composition within the atmosphere, the number of biological ice nuclei increased significantly during periods of high relative humidity. However, these changes in ice nuclei numbers were not associated with changes in the relative abundances of the most commonly studied ice-nucleating bacteria.
authors.affiliation: Department of Ecology and Evolutionary Biology, University of Colorado, Boulder, 80309, USA. robert.bowers@colorado.edu
authors.forename: Robert M
authors.initials: RM
authors.lastname: Bowers
authors.forename: Christian L
authors.initials: CL
authors.lastname: Lauber
authors.forename: Christine
authors.initials: C
authors.lastname: Wiedinmyer
authors.forename: Micah
authors.initials: M
authors.lastname: Hamady
authors.forename: Anna G
authors.initials: AG
authors.lastname: Hallar
authors.forename: Ray
authors.initials: R
authors.lastname: Fall
authors.forename: Rob
authors.initials: R
authors.lastname: Knight
authors.forename: Noah
authors.initials: N
authors.lastname: Fierer
mesh_terms: D000391:Air Microbiology; D001419:Bacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D044822:Biodiversity; D016000:Cluster Analysis; D015169:Colony Count, Microbial; D003120:Colorado; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005658:Fungi / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D007053:Ice; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012337:RNA, Ribosomal, 18S / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D004269:DNA, Bacterial; D004271:DNA, Fungal; D004275:DNA, Ribosomal; D007053:Ice; D012336:RNA, Ribosomal, 16S; D012337:RNA, Ribosomal, 18S