title: Pseudomonas cuatrocienegasensis sp. nov., isolated from an evaporating lagoon in the Cuatro Cienegas valley in Coahuila, Mexico.
abstract: Nine Gram-negative, rod-shaped, non-spore-forming isolates with identical or very similar repetitive-sequence-based PCR profiles were recovered from an evaporative lagoon in Mexico. Two strains, designated 1N(T) and 3N, had virtually identical 16S rRNA gene sequences and, on the basis of these sequences, were identified as members of the genus Pseudomonas, with Pseudomonas peli R-20805(T) as the closest relative. All nine isolates had practically identical whole-cell protein profiles. The major fatty acids [C(16 : 0,) C(18 : 1)omega7c and summed feature a (C(16 : 1)omega7 and/or C(16 : 1)omega6c)] of strains 1N(T) and 3N supported their affiliation with the genus Pseudomonas. The DNA-DNA reassociation values with respect to P. peli LMG 23201(T) and other closely related Pseudomonas species were <15 %. Physiological and biochemical tests allowed phenotypic differentiation of the strains analysed, including strain 1N(T), from the five phylogenetically closest Pseudomonas species. On the basis of the data obtained by using this polyphasic taxonomic approach, the nine strains represent a novel species, for which the name Pseudomonas cuatrocienegasensis sp. nov. is proposed. The type strain is 1N(T) (=LMG 24676(T)=CIP 109853(T)).
authors.affiliation: Departamento de Ecología Evolutiva, Instituto de Ecología, Universidad Nacional Autónoma de México, Apartado Postal 70-275, México DF 04510, Mexico.
authors.forename: Ana E
authors.initials: AE
authors.lastname: Escalante
authors.forename: Jesús
authors.initials: J
authors.lastname: Caballero-Mellado
authors.forename: Lourdes
authors.initials: L
authors.lastname: Martínez-Aguilar
authors.forename: Alejandra
authors.initials: A
authors.lastname: Rodríguez-Verdugo
authors.forename: Andrea
authors.initials: A
authors.lastname: González-González
authors.forename: Jeiry
authors.initials: J
authors.lastname: Toribio-Jiménez
authors.forename: Valeria
authors.initials: V
authors.lastname: Souza
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000032:analysis; D004275:DNA, Ribosomal / Q000032:analysis; D005227:Fatty Acids / Q000032:analysis; D005618:Fresh Water / Q000382:microbiology*; D005838:Genotype; D008800:Mexico; D008969:Molecular Sequence Data; D010641:Phenotype; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods; D011549:Pseudomonas / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013045:Species Specificity
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S