[Detection of Th17 cells in splenocytes of BALB/c mouse].
OBJECTIVE
To observe the role of IL-12 in Th17 cells differentiation.


METHODS
Mouse splenocytes were prepared and cultured with alphaCD3 alone or plus different concentration of recombined mice IL-12, 3 days later, IL-17 in the culture supernatant was assayed by ELISA. In addition, Mouse splenocytes were stimulated byalphaCD3 with or without IL-12 for 48 h, then cell were washed, and restimulated by PMA and Ionomycin, the expression of intracellular IL-17 and IFN-gamma by CD4(+) and CD8(+) T cells were examined at single cell level by Flow Cytometric analysis.


RESULTS
Th-17 cells is a independent cell population, which did not express IL-5, IL-10, IFN-gamma and Foxp3. IL-12 could induce active mouse T cells produce large amount of IFN-gamma and the differentiation toward Th1 cell. On the contrary, IL-12 could inhabit the production of IL-17 and differentiation toward Th17 cells.


CONCLUSIONS
IL-12 inhabit T cell differentiation toward Th17 cells.
Huang
Jun
J
Department of Pathogenic Biology and Immunology, Guangzhou Medical College, Guangzhou 510182, China.
Zhao
Jing-jing
JJ
Li
Jian
J
Li
Zi
Z
D000818:Animals; D015496:CD4-Positive T-Lymphocytes / Q000166:cytology / Q000378:metabolism; D018414:CD8-Positive T-Lymphocytes / Q000166:cytology / Q000378:metabolism; D002454:Cell Differentiation / Q000187:drug effects*; D002478:Cells, Cultured; D017077:Culture Media, Conditioned / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005434:Flow Cytometry; D007371:Interferon-gamma / Q000378:metabolism; D018664:Interleukin-12 / Q000494:pharmacology*; D020381:Interleukin-17 / Q000378:metabolism*; D015759:Ionomycin / Q000494:pharmacology; D051379:Mice; D008807:Mice, Inbred BALB C; D013154:Spleen / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D006377:T-Lymphocytes, Helper-Inducer / Q000166:cytology* / Q000378:metabolism; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D013997:Time Factors
D017077:Culture Media, Conditioned; D020381:Interleukin-17; D018664:Interleukin-12; D015759:Ionomycin; D007371:Interferon-gamma; D013755:Tetradecanoylphorbol Acetate