title: Sodium caseinate induces secretion of macrophage colony-stimulating factor from neutrophils.
abstract: In this work we provide evidence that granulocytes produce macrophage colony-stimulating factor (M-CSF) in the band cell stage and secrete it upon sodium caseinate-mediated differentiation to polymorphonuclear cells. We identified M-CSF in an enriched population of myeloid band cells from murine bone marrow using a chromophore-labeled monoclonal anti-M-CSF antibody. An ELISA assay was then used to detect secreted M-CSF in culture supernatants of enriched band cells differentiated to mature neutrophils using sodium caseinate. Colony formation in vitro by the supernatants from differentiating band cells was blocked by anti-M-CSF, thus suggesting that this factor is the only one responsible for this activity. Our data imply that casein can modulate hematopoiesis possibly via M-CSF production. Finally we discuss the possibility whether this M-CSF in concert with G-CSF could establish a cellular communication network between macrophages and granulocytes allowing them to simultaneously arrive at the inflammatory site.
authors.affiliation: Laboratorio de Hematopoyesis y Leucemia, Laboratorio 8, PB, UMIEZ. Campus II. Facultad de Estudios Superiores Zaragoza, UNAM, Batalla 5 de Mayo s/n, Col. E. Oriente, Esquina Fuerte Loreto, Iztapalapa, CP 09230, México, DF., México. edelmiro@servidor.unam.mx
authors.forename: E
authors.initials: E
authors.lastname: Santiago-Osorio
authors.forename: L
authors.initials: L
authors.lastname: Mora
authors.forename: M
authors.initials: M
authors.lastname: Bautista
authors.forename: J J
authors.initials: JJ
authors.lastname: Montesinos
authors.forename: I
authors.initials: I
authors.lastname: Martínez
authors.forename: G
authors.initials: G
authors.lastname: Ramos-Mandujano
authors.forename: R
authors.initials: R
authors.lastname: Zambrano
authors.forename: A
authors.initials: A
authors.lastname: Monroy-García
authors.forename: B
authors.initials: B
authors.lastname: Weiss-Steider
authors.forename: E
authors.initials: E
authors.lastname: Ledesma-Martínez
authors.forename: I
authors.initials: I
authors.lastname: Aguiñiga
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000187:drug effects / Q000502:physiology; D002364:Caseins / Q000494:pharmacology*; D002454:Cell Differentiation; D005260:Female; D016173:Macrophage Colony-Stimulating Factor / Q000378:metabolism*; D008297:Male; D051379:Mice; D009504:Neutrophils / Q000187:drug effects* / Q000378:metabolism
chemical_list: D002364:Caseins; D016173:Macrophage Colony-Stimulating Factor