title: [Fluorescent linear labelling method for biochip applications].
abstract: Fluorescent Linear Labelling technique is an effective, single-tube and linear amplification method. The sample cDNA was synthesized from total RNA, and was labelled antisense cRNA from double-stranded cDNA with T7RNA polymerase; it can be used for hybridization to oligonucleotide biochip. Fluorescent Linear Labelling Method can result in 50- to 100-fold higher degrees of amplification, and has been shown to retain information on transcript abundance, thus making it an efficient, robust technique for fluorescent labelling on biochip.
authors.affiliation: Institute of Genetic Engineering, Nanfang Medical University, Guangzhou 510515, China.
authors.forename: Dapeng
authors.initials: D
authors.lastname: Ding
authors.forename: Wenli
authors.initials: W
authors.lastname: Ma
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhang
authors.forename: Rong
authors.initials: R
authors.lastname: Shi
authors.forename: Jueyu
authors.initials: J
authors.lastname: Zhou
authors.forename: Wenling
authors.initials: W
authors.lastname: Zheng
mesh_terms: D005456:Fluorescent Dyes / Q000737:chemistry*; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D013194:Staining and Labeling / Q000379:methods*
chemical_list: D005456:Fluorescent Dyes