title: Application of polymerase chain reaction to analysis of antigen receptor rearrangements to support endoscopic diagnosis of canine alimentary lymphoma.
abstract: We evaluated the usefulness of polymerase chain reaction for antigen receptor gene rearrangement analysis (PARR) of endoscopic biopsy specimens for diagnosis of canine alimentary lymphoma. Two endoscopic biopsy specimens were obtained from each lesion in 78 dogs with gastrointestinal symptoms. One specimen was histopathologically examined by a pathologist, and the other was analyzed by PARR. All samples were categorized into three groups [lymphoma (n=4), adenocarcinoma (n=5) and enteritis groups (n=69)] based on the histopathological diagnosis. In the lymphoma group, one case was IgH major-positive, and three cases were TCRgamma-positive, representing clonal expansion of B- and T-cells, respectively. PARR produced negative results for all cases in the adenocarcinoma group. In the enteritis group, six cases were TCRgamma-positive. Two of the six TCRgamma-positive enteritis cases were cytologically diagnosed as lymphoma by fine needle aspiration during a laparotomy. In the enteritis group, the survival times were compared between the TCRgamma-positive and TCRgamma-negative cases. The overall survival time of the TCRgamma-positive enteritis cases was significantly shorter than that of the TCRgamma-negative enteritis cases according to a log-rank test (p<0.0001). With regard to other factors, such as age, clinical signs and the serum albumin concentration, there were no significant differences between the TCRgamma-positive and TCRgamma-negative enteritis cases. In conclusion, PARR is capable of detecting alimentary lymphoma and latent alimentary lymphoma, which cannot be histopathologically diagnosed using endoscopic biopsy specimens. Furthermore, a TCRgamma-positive result in PARR may imply a poor prognosis.
authors.affiliation: Faculty of Agriculture, Yamaguchi University, Yamaguchi, Japan.
authors.forename: Naoki
authors.initials: N
authors.lastname: Kaneko
authors.forename: Yoshifumi
authors.initials: Y
authors.lastname: Yamamoto
authors.forename: Yuko
authors.initials: Y
authors.lastname: Wada
authors.forename: Takako
authors.initials: T
authors.lastname: Shimokawa Miyama
authors.forename: Hiroko
authors.initials: H
authors.lastname: Hiraoka
authors.forename: Kazuhito
authors.initials: K
authors.lastname: Itamoto
authors.forename: Takuya
authors.initials: T
authors.lastname: Mizuno
authors.forename: Munekazu
authors.initials: M
authors.lastname: Nakaichi
authors.forename: Tomoko
authors.initials: T
authors.lastname: Takahashi
authors.forename: Toshihiro
authors.initials: T
authors.lastname: Watari
authors.forename: Masaru
authors.initials: M
authors.lastname: Okuda
mesh_terms: D000818:Animals; D001706:Biopsy / Q000662:veterinary; D004247:DNA / Q000737:chemistry / Q000235:genetics; D004283:Dog Diseases / Q000175:diagnosis / Q000235:genetics* / Q000473:pathology; D004285:Dogs; D016099:Endoscopy, Gastrointestinal / Q000662:veterinary; D005260:Female; D015334:Gene Rearrangement, gamma-Chain T-Cell Antigen Receptor; D006651:Histocytochemistry / Q000662:veterinary; D007414:Intestinal Neoplasms / Q000175:diagnosis / Q000235:genetics / Q000473:pathology / Q000662:veterinary*; D053208:Kaplan-Meier Estimate; D008223:Lymphoma / Q000175:diagnosis / Q000235:genetics / Q000473:pathology / Q000662:veterinary*; D008297:Male; D016133:Polymerase Chain Reaction / Q000662:veterinary*
chemical_list: D004247:DNA