Interaction between ascorbic acid and follicle-stimulating hormone maintains follicular viability after long-term in vitro culture of caprine preantral follicles.
This study evaluates the effects of ascorbic acid and its interaction with follicle-stimulating hormone (FSH) on the morphology, activation, and in vitro growth of caprine preantral follicles. Ovarian fragments were cultured for 1, 7, or 14 d in minimum essential medium (MEM) containing ascorbic acid (50 or 100microg/mL), FSH (50ng/mL), or both of these substances. Ovarian tissue that was either fresh (control) or cultured for 1, 7, or 14 d was processed for histological and ultrastructural evaluation. The results showed that after 14 d of culture, medium supplemented with 50microg/mL of ascorbic acid alone or combined with FSH showed higher rates of follicular survival compared with MEM. After 7 d of culture, FSH, ascorbic acid at 50microg/mL with or without FSH, and ascorbic acid at 100microg/mL increased the percentage of follicular activation compared to fresh control. In addition, FSH alone significantly increased the percentage of growing follicles after 14 d. The combination of 50microg/mL of ascorbic acid and FSH promoted a significant increase in oocyte and follicular diameter after 7 d of culture. Ultrastructural and fluorescent analysis confirmed the integrity of follicles cultured with 50microg/mL of ascorbic acid and FSH after 14 d. In conclusion, the combination of 50microg/mL of ascorbic acid and FSH maintained follicular integrity and promoted follicular activation and growth after long-term in vitro culture of caprine preantral follicles.
Rossetto
R
R
Faculty of Veterinary Medicine, UPIS - Pioneer Union of Social Integration, DF, Brazil. rafael.rossetto@hotmail.com
Lima-Verde
I B
IB
Matos
M H T
MH
Saraiva
M V A
MV
Martins
F S
FS
Faustino
L R
LR
Araújo
V R
VR
Silva
C M G
CM
Name
K P O
KP
SN
S N Báo
SN
Campello
C C
CC
Figueiredo
J R
JR
Blume
H
H
D000818:Animals; D001205:Ascorbic Acid / Q000008:administration & dosage*; D003470:Culture Media; D004347:Drug Interactions; D005260:Female; D005456:Fluorescent Dyes; D005640:Follicle Stimulating Hormone / Q000008:administration & dosage*; D006041:Goats; D046529:Microscopy, Electron, Transmission; D008856:Microscopy, Fluorescence; D009924:Organ Culture Techniques; D006080:Ovarian Follicle / Q000033:anatomy & histology / Q000187:drug effects / Q000502:physiology*; D013997:Time Factors
D003470:Culture Media; D005456:Fluorescent Dyes; D005640:Follicle Stimulating Hormone; D001205:Ascorbic Acid