[Antimicrobial activities of the culture supernatant of 293 cells transfected with recombinant HBD-2 gene eukyriotic expressive plasmid].
In this study, HBD-2 eukaryotic expression recombinant plasmid pcDNA 3.1-zeo(+)-HBD-2 was constructed by the molecular cloning technologies, and 293 cells were transfected by liposome. The cell culture supernatant was collected and its antibacterial activities against Staphylococcus aureus ATCC25923 and Pseudomonas aeruginosa ATCC27853 were tested. The antibacterial activity against S. aureus of the culture supernatant of the HBD-2 gene transfected-293 cells was detected, whereas in the same condition, the antibacterial activity against P. aeruginosa was not detected. This result suggested that HBD-2 may preferentially kill Staphylococcus aureus.
Wang
Hui
H
Department of Geriatrics, West China Hospital, Sichuan University, Chengdu 610041, China.
Dong
Birong
B
Zhou
Yan
Y
D000890:Anti-Infective Agents / Q000494:pharmacology*; D002460:Cell Line; D005822:Genetic Vectors; D006801:Humans; D007668:Kidney / Q000166:cytology* / Q000196:embryology; D010957:Plasmids; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology*; D014162:Transfection; D023083:beta-Defensins / Q000235:genetics / Q000378:metabolism* / Q000494:pharmacology
D000890:Anti-Infective Agents; C106641:DEFB4A protein, human; D011994:Recombinant Proteins; D023083:beta-Defensins