title: Use of the DNA Checkerboard hybridization method for detection and quantitation of Candida species in oral microbiota.
abstract: The DNA Checkerboard method enables the simultaneous identification of distinct microorganisms in a large number of samples and employs up to 45 whole genomic DNA probes to gram-negative and gram-positive bacterial species present in subgingival biofilms. Collectively, they account for 55%-60% of the bacteria in subgingival biofilms. In this study, we present the DNA Checkerboard hybridization as an alternative method for the detection and quantitation of Candida species in oral cavities. Our results reveal that DNA Checkerboard is sensitive enough and constitutes a powerful and appropriate method for detecting and quantifying Candida species found in the oral cavity.
authors.affiliation: Ribeirão Preto Dental School, University of São Paulo, Department of Dental Materials and Prosthodontics, Avenida do Café S/N, Monte Alegre, Ribeirão Preto, São Paulo, Brazil.
authors.forename: Cássio
authors.initials: C
authors.lastname: do Nascimento
authors.forename: Rubens
authors.initials: R
authors.lastname: Ferreira de Albuquerque Junior
authors.forename: João Paulo Mardegan
authors.initials: JP
authors.lastname: Issa
authors.forename: Izabel Yoko
authors.initials: IY
authors.lastname: Ito
authors.forename: Cláudia Helena
authors.initials: CH
authors.lastname: Lovato da Silva
authors.forename: Helena
authors.initials: H
authors.lastname: de Freitas Oliveira Paranhos
authors.forename: Raphael Freitas
authors.initials: RF
authors.lastname: de Souza
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D018441:Biofilms; D002175:Candida / Q000235:genetics* / Q000302:isolation & purification*; D002177:Candidiasis / Q000382:microbiology*; D015169:Colony Count, Microbial / Q000379:methods*; D004271:DNA, Fungal / Q000235:genetics*; D005260:Female; D005881:Gingiva / Q000382:microbiology; D006801:Humans; D008297:Male; D009693:Nucleic Acid Hybridization / Q000379:methods*; D012680:Sensitivity and Specificity
chemical_list: D004271:DNA, Fungal