Molecular diagnosis in allergology: application of the microarray technique.
Recombinant and purified allergens are currently available for determining specific IgE targeted to different allergenic components. In this way it is possible to diagnose the sensitization profile of each individual patient. The microarray technique makes it possible to determine specific IgE against multiple allergens simultaneously in one same patient, with a minimum amount of serum, and even allows the determination of IgG and IgM against the same allergens in one same serum sample. Microarray procedures are being developed not only for the determination of antibodies but also for cell activation tests. In addition, microarray technology will help explain cross-reactions, and will facilitate the evaluation of subjects in which skin tests cannot be performed. These techniques will allow a great step forward in the development of immunotherapy specifically targeted to the sensitizations found in each individual patient, yielding especially hypoallergenic forms of great immunogenic capacity, and thus improving the safety and efficacy of immunotherapy. Lastly, microarrays will improve our understanding of the physiopathology of allergic diseases.
Ferrer
M
M
Departamento de Alergia e Immunología Clinica, Clinica Universidad de Navarra, Pamplona, Spain. mferrerp@unav.es
Sanz
M L
ML
Sastre
J
J
Bartra
J
J
del Cuvillo
A
A
Montoro
J
J
Jáuregui
I
I
Dávila
I
I
Mullol
J
J
Valero
A
A
D000485:Allergens / Q000276:immunology*; D006801:Humans; D006967:Hypersensitivity / Q000175:diagnosis* / Q000276:immunology; D007073:Immunoglobulin E / Q000032:analysis / Q000276:immunology*; D040081:Protein Array Analysis / Q000379:methods*; D011994:Recombinant Proteins / Q000737:chemistry / Q000276:immunology
D000485:Allergens; D011994:Recombinant Proteins; D007073:Immunoglobulin E