Authentication of the family Polygonaceae in Chinese pharmacopoeia by DNA barcoding technique.
BACKGROUND
Medicinal plants belonging to the family Polygonaceae in Chinese pharmacopoeia possess important medicinal efficacy in traditional Chinese medicines.


OBJECTIVE
DNA barcodes are first used to discriminate the Polygonaceae in Chinese pharmacopoeia and their adulterants.


METHODS
DNA samples, extracted from thirty-eight specimens belonging to eighteen species in Polygonaceae, were used as templates. Eight candidate barcodes were amplified by polymerase chain reaction. Sequence analysis was accomplished by CodonCode Aligner V 2.06 and DNAman V 6. Species identification was performed using MEGA V 4.0.


RESULTS
The amplification efficiency of six candidate DNA barcodes (rbcL, trnH-psbA, ndhJ, rpoB, rpoC1, accD) was 100%, while the efficiency of YCF5 and nrITS was 56% and 44%, respectively. The interspecific divergence was highest for the trnH-psbA (20.05%), followed by the nrITS (14.01%) across all species pairs, while intraspecific variation both within populations and between populations was absent (0.0%). The trnH-psbA can not only distinguish ten species of Polygonaceae in Chinese pharmacopoeia, but also recognize eight other species of Polygonaceae including their adulterants.


CONCLUSIONS
Our findings show that DNA barcoding is an efficient tool for identification of Polygonaceae in Chinese pharmacopoeia and their adulterants.
Song
Jingyuan
J
Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Peking Union Medical College, 151 Malianwa North Road, Haidian District, Beijing 100193, PR China.
Yao
Hui
H
Li
Ying
Y
Li
Xiwen
X
Lin
Yulin
Y
Liu
Chang
C
Han
Jianping
J
Xie
Caixiang
C
Chen
Shilin
S
D002681:China; D017931:DNA Primers; D018744:DNA, Plant / Q000737:chemistry / Q000145:classification*; D004340:Drug Contamination; D001330:Electronic Data Processing; D008516:Medicine, Chinese Traditional; D010602:Pharmacopoeias as Topic; D019664:Polygonaceae / Q000737:chemistry / Q000145:classification* / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013045:Species Specificity
D017931:DNA Primers; D018744:DNA, Plant