Antiviral and anticancer activities of 13(E)-labd-13-ene-8alpha,15-diol isolated from Brachyglottis monroi.
The antiviral activity of 13(E)-labd-13-ene-8alpha,15-diol (1), isolated from Brachyglottis monroi, was examined against human rhinovirus 2 (HRV2) and 3 (HRV3), and the anticancer activity on human cancer cells (A549 and Hep2). Compound (1) showed strong anti-HRV2 and HRV3 activity with a 50% inhibitory concentration (IC(50)) of 2.68 and 0.87 microg/mL, respectively, and a 50% cytotoxicity concentration (CC(50)) of 59.45 microg/mL. Ribavirin only showed anti-HRV3 activity with an IC(50) of 30.48 microg/mL and a CC(50) > 100 microg/mL. The addition of compound (1) to HRV-infected HeLa cells directly reduced the formation of visible cytopathic effect (CPE) and it directly interacted with HRV particles. Furthermore, A549 and Hep2 cells incubated with 32 microg/mL of compound (1) for 48 h exhibited antilung and antilaryngeal cancer activities, with a viability of less than 50%. These results suggest that compound (1) may be used as a potential antiviral and anticancer agent.
Choi
Hwa Jung
HJ
Natural Medicines Research Centre, Korea Research Institute of Bioscience and Biotechnology, Daejeon, Republic of Korea.
Song
Jae Hyoung
JH
Kwon
Dur Han
DH
Baek
Seung Hwa
SH
D000972:Antineoplastic Agents, Phytogenic / Q000302:isolation & purification / Q000494:pharmacology*; D000998:Antiviral Agents / Q000302:isolation & purification / Q000494:pharmacology*; D019659:Asteraceae / Q000737:chemistry*; D004224:Diterpenes / Q000494:pharmacology*; D006367:HeLa Cells; D006801:Humans; D020128:Inhibitory Concentration 50; D012229:Rhinovirus / Q000187:drug effects
D000972:Antineoplastic Agents, Phytogenic; D000998:Antiviral Agents; D004224:Diterpenes; C440092:labd-13(E)-ene,-8alpha,15-diol