title: Potency fingerprint of herbal products Danshen injection for their quality evaluation.
abstract: The fingerprint technique has been studied frequently as a useful strategy for quality of traditional Chinese medicine. A novel potency fingerprint that can quantitatively analyze the antioxidant activity of individual constituent and provide the total antioxidant activities of the samples has been developed by high-performance liquid chromatography coupled with ultraviolet and pyrogallol-luminol chemiluminescence detection (HPLC-diode array detection (DAD)-PLD). Hierarchical clustering analysis has been used as a powerful pattern recognition tool to identify and classify Danshen injection from different factories. In addition, the combination use of the chromatographic fingerprint and potency fingerprint with principal component analysis was applied to quality control of Danshen injection. The results demonstrated that the proposed potency fingerprint was a useful means to control the quality and to clarify the possible mechanism of action of herbal products.
authors.affiliation: The Key Laboratory for Prescription of National Educational Ministry of China, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China.
authors.forename: Yan-Xu
authors.initials: YX
authors.lastname: Chang
authors.forename: Dong-Mei
authors.initials: DM
authors.lastname: Yan
authors.forename: Lin-Lin
authors.initials: LL
authors.lastname: Chen
authors.forename: Xiao-Ping
authors.initials: XP
authors.lastname: Ding
authors.forename: Jin
authors.initials: J
authors.lastname: Qi
authors.forename: Li-Yuan
authors.initials: LY
authors.lastname: Kang
authors.forename: Bo-Li
authors.initials: BL
authors.lastname: Zhang
authors.forename: Bo-Yang
authors.initials: BY
authors.lastname: Yu
mesh_terms: D000975:Antioxidants / Q000737:chemistry* / Q000592:standards; D002851:Chromatography, High Pressure Liquid; D003627:Data Interpretation, Statistical; D004365:Drugs, Chinese Herbal / Q000737:chemistry* / Q000592:standards; D007202:Indicators and Reagents; D007267:Injections; D049449:Luminescence; D010634:Phenobarbital / Q000737:chemistry; D025341:Principal Component Analysis; D011748:Pyrogallol / Q000737:chemistry; D011786:Quality Control; D017382:Reactive Oxygen Species / Q000737:chemistry; D027561:Salvia miltiorrhiza / Q000737:chemistry*; D012996:Solutions; D013056:Spectrophotometry, Ultraviolet
chemical_list: D000975:Antioxidants; D004365:Drugs, Chinese Herbal; D007202:Indicators and Reagents; D017382:Reactive Oxygen Species; D012996:Solutions; D011748:Pyrogallol; D010634:Phenobarbital