title: The S. pombe mitotic regulator Cut12 promotes spindle pole body activation and integration into the nuclear envelope.
abstract: The fission yeast spindle pole body (SPB) comprises a cytoplasmic structure that is separated from an ill-defined nuclear component by the nuclear envelope. Upon mitotic commitment, the nuclear envelope separating these domains disperses as the two SPBs integrate into a hole that forms in the nuclear envelope. The SPB component Cut12 is linked to cell cycle control, as dominant cut12.s11 mutations suppress the mitotic commitment defect of cdc25.22 cells and elevated Cdc25 levels suppress the monopolar spindle phenotype of cut12.1 loss of function mutations. We show that the cut12.1 monopolar phenotype arises from a failure to activate and integrate the new SPB into the nuclear envelope. The activation of the old SPB was frequently delayed, and its integration into the nuclear envelope was defective, resulting in leakage of the nucleoplasm into the cytoplasm through large gaps in the nuclear envelope. We propose that these activation/integration defects arise from a local deficiency in mitosis-promoting factor activation at the new SPB.
authors.affiliation: Cancer Research UK Cell Division Group, Paterson Institute for Cancer Research, University of Manchester, Manchester M204BX, England, UK.
authors.forename: Victor A
authors.initials: VA
authors.lastname: Tallada
authors.forename: Kenji
authors.initials: K
authors.lastname: Tanaka
authors.forename: Mitsuhiro
authors.initials: M
authors.lastname: Yanagida
authors.forename: Iain M
authors.initials: IM
authors.lastname: Hagan
mesh_terms: D002467:Cell Nucleus / Q000378:metabolism / Q000648:ultrastructure; D003593:Cytoplasm / Q000378:metabolism / Q000648:ultrastructure; D008869:Microtubule-Associated Proteins / Q000502:physiology*; D008870:Microtubules / Q000378:metabolism / Q000648:ultrastructure; D008938:Mitosis / Q000502:physiology; D008954:Models, Biological; D009685:Nuclear Envelope / Q000378:metabolism* / Q000648:ultrastructure; D010750:Phosphoproteins / Q000502:physiology*; D012568:Schizosaccharomyces / Q000166:cytology / Q000235:genetics / Q000378:metabolism*; D029702:Schizosaccharomyces pombe Proteins / Q000502:physiology*; D008941:Spindle Apparatus / Q000378:metabolism*
chemical_list: C111708:Cut12 protein, S pombe; D008869:Microtubule-Associated Proteins; D010750:Phosphoproteins; D029702:Schizosaccharomyces pombe Proteins