Qualitative electron microscopic analysis of cultured chick embryonic cardiac and skeletal muscle cells: the cellular effect of coenzyme q10 after exposure to triton x-100.
The numerous protective effects of coenzyme Q10 (CoQ10) evoked the question of whether it might be able to elicit protection to cell membranes after being challenged by the membrane disrupter Triton X-100. Cardiac and skeletal muscle tissue from chick embryos was cultured and exposed to increasing concentrations of CoQ10 and Triton X-100. Scanning electron microscopy was used to study cell morphology. Results suggested the ability of CoQ10 to offer protection to cells challenged by Triton X-100. The authors suggest that CoQ10 may offer protection to muscle cells, by enhancing membrane repair via patch formation by an unknown mechanism that possibly involves Ca(2+)-dependent ion channel activation.
Potgieter
Marnie
M
Department of Anatomy, University of Pretoria, Pretoria, Gauteng, South Africa.
Pretorius
Etheresia
E
Oberholzer
Hester Magdelena
HM
D000818:Animals; D015220:Calcium Channels / Q000187:drug effects; D020013:Calcium Signaling; D002462:Cell Membrane / Q000187:drug effects; D002478:Cells, Cultured; D002642:Chick Embryo; D047108:Embryonic Development; D008855:Microscopy, Electron, Scanning; D018485:Muscle Fibers, Skeletal / Q000187:drug effects* / Q000648:ultrastructure; D032383:Myocytes, Cardiac / Q000187:drug effects* / Q000648:ultrastructure; D017830:Octoxynol / Q000633:toxicity*; D013501:Surface-Active Agents / Q000633:toxicity*; D014451:Ubiquinone / Q000031:analogs & derivatives* / Q000494:pharmacology; D014815:Vitamins / Q000494:pharmacology*
D015220:Calcium Channels; D013501:Surface-Active Agents; D014815:Vitamins; D014451:Ubiquinone; D017830:Octoxynol; C024989:coenzyme Q10