Long-term survival of Salmonella enterica serovar Typhimurium reveals an infectious state that is underrepresented on laboratory media containing bile salts.
Cells in desiccated Salmonella enterica serovar Typhimurium rdar (red, dry, and rough) morphotype colonies were examined for culturability and infectivity after 30 months. Culturability decreased only 10-fold; however, cells were underrepresented on Salmonella selective media containing bile salts. These cells were mildly attenuated compared to the infectivity of freshly grown cells but still able to cause systemic infections in mice.
Apel
Dmitry
D
Department of Microbiology and Infectious Diseases, Faculty of Medicine, University of Calgary, 3330 Hospital Dr. NW, Calgary, Canada.
White
Aaron P
AP
Grassl
Guntram A
GA
Finlay
B Brett
BB
Surette
Michael G
MG
D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001647:Bile Acids and Salts / Q000494:pharmacology; D015169:Colony Count, Microbial; D003470:Culture Media / Q000737:chemistry; D051379:Mice; D008810:Mice, Inbred C57BL; D050296:Microbial Viability; D012480:Salmonella Infections / Q000382:microbiology*; D012486:Salmonella typhimurium / Q000302:isolation & purification* / Q000472:pathogenicity*
D000900:Anti-Bacterial Agents; D001647:Bile Acids and Salts; D003470:Culture Media