title: Key role of teichoic acids on aflatoxin B binding by probiotic bacteria.
abstract: OBJECTIVE
  To assess the ability of five probiotic bacteria to bind aflatoxin B(1) and to determine the key role of teichoic acids in the binding mechanism.
  
  
  RESULTS
  The strains were incubated in aqueous solutions containing aflatoxin B(1) (AFB(1)). The amount of free toxin was quantified by HPLC. Stability of the bacteria-aflatoxin complex was evaluated by repeated washes with buffer. In order to understand the binding process, protoplasts, spheroplasts and cell wall components of two strains were analysed to assess their capacity to bind AFB(1). Additionally, the role of teichoic acids in the AFB(1) binding process was assessed. Lactobacillus reuteri strain NRRL14171 and Lactobacillus casei strain Shirota were the most efficient strains for binding AFB(1). The stability of the AFB(1)-bacteria complex appears to be related to the binding ability of a particular strain; AFB(1) binding was also pH-dependent. Our results suggest that teichoic acids could be responsible for this ability.
  
  
  CONCLUSIONS
  Our results provide information concerning AFB(1) binding by previously untested strains, leading to enhanced understanding of the mechanism by which probiotic bacteria bind AFB(1).
  
  
  CONCLUSIONS
  Our results support the suggestion that some probiotic bacteria could prevent absorption of aflatoxin from the gastrointestinal tract.
authors.affiliation: UNIDA-Instituto Tecnológico de Veracruz, Col. Formando Hogar, Veracruz, México.
authors.forename: A
authors.initials: A
authors.lastname: Hernandez-Mendoza
authors.forename: D
authors.initials: D
authors.lastname: Guzman-de-Peña
authors.forename: H S
authors.initials: HS
authors.lastname: Garcia
mesh_terms: D016604:Aflatoxin B1 / Q000378:metabolism*; D001422:Bacterial Adhesion / Q000187:drug effects*; D002473:Cell Wall / Q000187:drug effects*; D002851:Chromatography, High Pressure Liquid; D007780:Lacticaseibacillus casei / Q000378:metabolism*; D052200:Limosilactobacillus reuteri / Q000378:metabolism*; D019936:Probiotics; D011523:Protoplasts / Q000187:drug effects; D013104:Spheroplasts / Q000187:drug effects; D013682:Teichoic Acids / Q000378:metabolism*; D013997:Time Factors
chemical_list: D013682:Teichoic Acids; D016604:Aflatoxin B1