Assessment of the total effective xenoestrogen burden in extracts of human placentas.
We have standardized a method to assess the total effective xenoestrogen burden (TEXB) in human placentas by the extraction and separation by high-performance liquid chromatography of two fractions containing lipophilic xenoestrogens (alpha) and endogenous hormones (beta), followed by assessing their estrogenicity in MCF-7 breast cancer cell-based E-Screen and Yeast Estrogen Screen (YES) bioassays. The means of TEXB alpha concentrations (in estradiol equivalent (Eeq) units) were 1.32 and 0.77 Eeq pM g(-1) placenta in the E-Screen and YES, respectively; TEXB beta concentrations were 6.97 and 11.56 Eeq pM g(-1) placenta, respectively. The interclass correlation coefficient was low and a fair level of agreement was observed after kappa test correction. According to the E-Screen and YES, TEXB alpha was > or = LOD in 70.0 and 55.0% of the placentas and 92.5 and 82.5% in beta, respectively. Although both bioassays can be recommended for assessing TEXB, there is greater experience with the use of the E-Screen for estrogenic assessment after extensive extraction of complex human matrices.
Lopez-Espinosa
Maria-Jose
MJ
Laboratory of Medical Investigations, San Cecilio University Hospital, University of Granada, CIBER Epidemiología y Salud Pública, Granada, Spain.
Silva
Elisabete
E
Granada
Alicia
A
Molina-Molina
Jose-Manuel
JM
Fernandez
Mariana F
MF
Aguilar-Garduño
Clemente
C
Olea-Serrano
Fatima
F
Kortenkamp
Andreas
A
Olea
Nicolas
N
D000328:Adult; D001681:Biological Assay / Q000592:standards*; D002457:Cell Extracts / Q000737:chemistry*; D045744:Cell Line, Tumor; D002851:Chromatography, High Pressure Liquid; D004967:Estrogens / Q000032:analysis*; D005260:Female; D006801:Humans; D010920:Placenta / Q000737:chemistry*; D011247:Pregnancy; D015262:Xenobiotics
D002457:Cell Extracts; D004967:Estrogens; D015262:Xenobiotics