title: Antioxidant sensors based on iron diethylenetriaminepentaacetic acid, hematin, and hemoglobin modified TiO2 nanoparticle printed electrodes.
abstract: Antioxidant amperometric sensors based on iron-containing complexes and protein modified electrodes were developed. Indium tin oxide glass was printed with TiO(2) nanoparticles, onto which iron-containing compounds and protein were adsorbed. When applied with negative potentials, the dissolved oxygen is reduced to H(2)O(2) at the electrode surface, and the H(2)O(2) generated in situ oxidizes Fe(II) to Fe(III), and then electrochemical reduction of Fe(III) therefore gives rise to a catalytic current. In the presence of antioxidants, H(2)O(2) was scavenged, the catalytic current was reduced, and the decreased current signal was proportional to the quantity of existing antioxidants. A kinetic model was proposed to quantify the H(2)O(2) scavenging capacities of the antioxidants. With the use of the sensor developed here, antioxidant measurements can be done quite simply: put the sensor into the sample solutions (in aerobic atmosphere), perform a cathodic polarization scan, and then read the antioxidant activity values. The present work can be complementary to the previous studies of antioxidant sensor techniques based on OH radicals and superoxide ions scavenging methods, but the sensor developed here is much easier to fabricate and use.
authors.affiliation: Department of Chemistry, Liaocheng University, Liaocheng, 252059, Shandong, China.
authors.forename: Qingqing
authors.initials: Q
authors.lastname: Guo
authors.forename: Shujun
authors.initials: S
authors.lastname: Ji
authors.forename: Qiaoli
authors.initials: Q
authors.lastname: Yue
authors.forename: Lei
authors.initials: L
authors.lastname: Wang
authors.forename: Jifeng
authors.initials: J
authors.lastname: Liu
authors.forename: Jianbo
authors.initials: J
authors.lastname: Jia
mesh_terms: D000975:Antioxidants / Q000032:analysis* / Q000737:chemistry; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D004566:Electrodes; D006427:Hemin / Q000737:chemistry*; D006454:Hemoglobins / Q000737:chemistry*; D006861:Hydrogen Peroxide / Q000378:metabolism; D007501:Iron / Q000737:chemistry / Q000378:metabolism*; D007700:Kinetics; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D010084:Oxidation-Reduction; D004369:Pentetic Acid / Q000031:analogs & derivatives* / Q000737:chemistry; D011199:Potentiometry; D017971:Tin Compounds / Q000737:chemistry; D014025:Titanium / Q000737:chemistry*
chemical_list: D000975:Antioxidants; D006454:Hemoglobins; D017971:Tin Compounds; C060068:diethylenetriaminetetraacetic acid; C009495:titanium dioxide; C109984:indium tin oxide; D006427:Hemin; D004369:Pentetic Acid; D006861:Hydrogen Peroxide; D014025:Titanium; D007501:Iron