title: Identification of phagocytic cells, NK-like cytotoxic cell activity and the production of cellular exudates in the coelomic cavity of adult zebrafish.
abstract: Coelomic cavity (CC) cells of mature zebrafish harvested by lavage with media or trypsin-EDTA contained 0.80-1.20 x 10(5) and 2.0-3.5 x 10(5) cells, respectively. Media lavage was composed of granulocytes (60-80%), lymphocytes (10-20%), and NCC (4-10%). Granulocytes had large electron dense cytoplasmic paracrystalline granules and a segmented nucleus; they expressed plastin-1, myeloid specific peroxidase and MCSF mRNA; and they were NCAMP-1(+). Lymphocytes had B- and T-cell specific mRNA and were NCAMP-1(-) and NCCRP-1(-). NCC were 3 microm, NCAMP-1(+) and NCCRP-1(+) and did not express B- and T-cell specific mRNA. Additionally, trypsin lavage contained monocytes (marginated chromatin, low nuclear:cytoplasm ratio, sparse cytosolic granules) and macrophages (non-segmented nuclei, no margination of chromatin, abundant electron dense granules). E. coli injected into the CC were phagocytosed in a dose and time dependent fashion by granulocytes, monocytes and macrophages. NCC lysed mammalian target cells and NCAMP-1 expressing hybridoma cells in redirected lysis assays.
authors.affiliation: Department of Infectious Diseases, College of Veterinary Medicine, University of Georgia, Athens, GA 30602, USA.
authors.forename: Lauren D
authors.initials: LD
authors.lastname: Moss
authors.forename: Margaret M
authors.initials: MM
authors.lastname: Monette
authors.forename: Liliana
authors.initials: L
authors.lastname: Jaso-Friedmann
authors.forename: John H
authors.initials: JH
authors.lastname: Leary
authors.forename: Scott T
authors.initials: ST
authors.lastname: Dougan
authors.forename: Thomas
authors.initials: T
authors.lastname: Krunkosky
authors.forename: Donald L
authors.initials: DL
authors.lastname: Evans
mesh_terms: D034841:Abdominal Cavity; D000818:Animals; D045744:Cell Line, Tumor; D002478:Cells, Cultured; D003601:Cytotoxicity Tests, Immunologic; D003602:Cytotoxicity, Immunologic / Q000276:immunology*; D004926:Escherichia coli / Q000276:immunology; D005122:Exudates and Transudates / Q000378:metabolism*; D005260:Female; D005434:Flow Cytometry; D015870:Gene Expression; D018922:HL-60 Cells; D006801:Humans; D020014:K562 Cells; D007694:Killer Cells, Natural / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D007962:Leukocytes / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D018613:Microscopy, Confocal; D008854:Microscopy, Electron; D010586:Phagocytes / Q000166:cytology / Q000276:immunology* / Q000648:ultrastructure; D010587:Phagocytosis / Q000276:immunology; D011946:Receptors, Antigen / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015027:Zebrafish / Q000276:immunology* / Q000378:metabolism; D029961:Zebrafish Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D011946:Receptors, Antigen; D029961:Zebrafish Proteins; C110644:nonspecific cytotoxic cell receptor protein 1