Reduced ability of hemagglutinin of the CAM-70 measles virus vaccine strain to use receptors CD46 and SLAM.
The CAM-70 measles virus (MV) vaccine strain is currently used for vaccination against measles. We examined the fusion-inducing ability of the CAM-70 hemagglutinin (H) protein and found that it was impaired in both CD46- and signaling lymphocyte activation molecule (SLAM)-expressing cells. We also generated recombinant MVs possessing H genes derived from the CAM-70 strain. The CAM-70 H protein impaired viral growth in both CD46- and SLAM-expressing cells. In peripheral blood lymphocytes (PBL) and monocyte-derived dendritic cells (Mo-DC), the CAM-70 strain did not grow efficiently. Infection with recombinant MVs revealed that impaired growth of the CAM-70 strain was attributed to the H gene only partly in PBL and largely in Mo-DC. Thus, impaired fusion-inducing ability of the H protein may be one of the underlying molecular mechanisms resulting in the attenuation of the CAM-70 strain.
Kato
Seiichi
S
Department of Virology, Osaka City University Medical School, Osaka 545-8585, Japan.
Ohgimoto
Shinji
S
Sharma
Luna Bhatta
LB
Kurazono
Sekiko
S
Ayata
Minoru
M
Komase
Katsuhiro
K
Takeda
Makoto
M
Takeuchi
Kaoru
K
Ihara
Toshiaki
T
Ogura
Hisashi
H
D019943:Amino Acid Substitution / Q000235:genetics; D000818:Animals; D015703:Antigens, CD / Q000378:metabolism*; D002478:Cells, Cultured; D002522:Chlorocebus aethiops; D003713:Dendritic Cells / Q000821:virology; D006367:HeLa Cells; D006389:Hemagglutinins, Viral / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D008214:Lymphocytes / Q000821:virology; D008459:Measles virus / Q000235:genetics / Q000502:physiology*; D051916:Membrane Cofactor Protein / Q000378:metabolism*; D008969:Molecular Sequence Data; D011485:Protein Binding; D011956:Receptors, Cell Surface / Q000378:metabolism*; D011991:Receptors, Virus / Q000378:metabolism*; D017422:Sequence Analysis, DNA; D000071177:Signaling Lymphocytic Activation Molecule Family Member 1; D014709:Vero Cells; D053585:Virus Attachment
D015703:Antigens, CD; C497857:CD46 protein, human; D006389:Hemagglutinins, Viral; D051916:Membrane Cofactor Protein; D011956:Receptors, Cell Surface; D011991:Receptors, Virus; C032397:hemagglutinin protein G, measles virus; D000071177:Signaling Lymphocytic Activation Molecule Family Member 1