title: Mouririelliptica: validation of gastroprotective, healing and anti-Helicobacter pylori effects.
abstract: BACKGROUND
  Mouriri elliptica Martius (Melastomataceae) is species reputed in folk medicine to heal gastric ulcer and gastritis.
  
  
  OBJECTIVE
  Methanolic extract (ME) and ethyl acetate fraction (EAF) from leaves of Mouriri elliptica were evaluated for their gastroprotective, healing, immunological, toxicological and anti-Helicobacter pylori activities.
  
  
  METHODS
  The gastroprotective action of ME and EAF was evaluated in rodent experimental models and to elucidate mechanisms of action, the antisecretory action, involvements of NO, SH, PGE(2), anti-Helicobacter pylori action of ME was evaluated. We also used immunohistochemical (PCNA and COX-2) and immunomodulatory (murine peritoneal macrophages) assays to evaluate Mouriri elliptica effects.
  
  
  RESULTS
  ME present gastroprotective action without antisecretory effect. Otherwise, ME showed anti-Helicobacter pylori action (MIC=0.025mug/mL) and was able to inhibit NO production by macrophages. This species also accelerate the healing of ulcerated gastric mucosa by stimulating proliferation factors (PCNA), COX-2 and maintained basal PGE(2) level independent action of NSAID in gastric mucosa. The phytochemical investigation showed that this species possesses phenolic acid derivatives, acylglycoflavonoids and condensed tannins which probably influenced their pharmacological action.
  
  
  CONCLUSIONS
  All these results suggest the efficacy and safety of Mouriri elliptica in combating and healing gastric ulcer.
authors.affiliation: São Paulo State University-Departamento de Fisiologia, Instituto de Biociências, Cp 610, CEP 18618-000, UNESP, Botucatu, SP, Brazil.
authors.forename: Fábio Cruz
authors.initials: FC
authors.lastname: Moleiro
authors.forename: Márcio Aparecido
authors.initials: MA
authors.lastname: Andreo
authors.forename: Raquel de Cássia Dos
authors.initials: Rde C
authors.lastname: Santos
authors.forename: Thiago de Mello
authors.initials: Tde M
authors.lastname: Moraes
authors.forename: Clenilson Martins
authors.initials: CM
authors.lastname: Rodrigues
authors.forename: Camila Bernardes de Andrade
authors.initials: CB
authors.lastname: Carli
authors.forename: Flávia Cristine Mascia
authors.initials: FC
authors.lastname: Lopes
authors.forename: Cláudia Helena
authors.initials: CH
authors.lastname: Pellizzon
authors.forename: Iracilda Zeppone
authors.initials: IZ
authors.lastname: Carlos
authors.forename: Tais Maria
authors.initials: TM
authors.lastname: Bauab
authors.forename: Wagner
authors.initials: W
authors.lastname: Vilegas
authors.forename: Clélia Akiko
authors.initials: CA
authors.lastname: Hiruma-Lima
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000009:adverse effects / Q000494:pharmacology / Q000627:therapeutic use; D000894:Anti-Inflammatory Agents, Non-Steroidal; D000897:Anti-Ulcer Agents / Q000009:adverse effects / Q000494:pharmacology / Q000627:therapeutic use*; D051546:Cyclooxygenase 2 / Q000378:metabolism; D015232:Dinoprostone / Q000378:metabolism; D004195:Disease Models, Animal; D000431:Ethanol; D005260:Female; D005419:Flavonoids / Q000009:adverse effects / Q000494:pharmacology / Q000627:therapeutic use; D005753:Gastric Mucosa / Q000187:drug effects* / Q000378:metabolism; D016481:Helicobacter Infections / Q000139:chemically induced / Q000188:drug therapy*; D016480:Helicobacter pylori / Q000187:drug effects*; D008264:Macrophages / Q000187:drug effects; D008297:Male; D035322:Melastomataceae / Q000737:chemistry; D051379:Mice; D009569:Nitric Oxide / Q000037:antagonists & inhibitors; D010636:Phenols / Q000009:adverse effects / Q000494:pharmacology / Q000627:therapeutic use; D008517:Phytotherapy; D010936:Plant Extracts / Q000009:adverse effects / Q000494:pharmacology / Q000627:therapeutic use*; D018515:Plant Leaves; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism; D051381:Rats; D013276:Stomach Ulcer / Q000139:chemically induced / Q000188:drug therapy* / Q000382:microbiology; D013634:Tannins / Q000009:adverse effects / Q000494:pharmacology / Q000627:therapeutic use
chemical_list: D000900:Anti-Bacterial Agents; D000894:Anti-Inflammatory Agents, Non-Steroidal; D000897:Anti-Ulcer Agents; D005419:Flavonoids; D010636:Phenols; D010936:Plant Extracts; D018809:Proliferating Cell Nuclear Antigen; D013634:Tannins; D009569:Nitric Oxide; D000431:Ethanol; D051546:Cyclooxygenase 2; D015232:Dinoprostone