Transient receptor potential A1 increase glutamate release on brain stem neurons.
Low temperature and noxious chemicals activate transient receptor potential (TRP) A1 channel in spinal nociceptive neurons. TRPA1 ligands are found as common ingredients of pungent spices and can trigger autonomic reflexes and alter blood pressure. Recent discovery of TRPA1 on visceral afferent pathway suggests such nonnociceptive responses may be closely related to TRPA1. To understand potential contributions of TRPA1 on autonomic response, we have tested TRPA1 agonist allyl isothiocyanate (AITC) effects on mechanically dispersed nucleus tractus solitarii neurons. AITC of 200 microM increased glutamate release in 58% of tested neurons and TRP channel antagonist ruthenium red (50 microM) blocked the AITC actions. tetrodotoxin and Cd did not eliminate AITC-evoked glutamate release increase. TRPA1 expressed in capsaicin-sensitive and insensitive neurons.
Sun
Boram
B
Department of Physiology and Medical Research Center, School of Medicine, Graduate School, Kyung Hee University, Seoul, Korea.
Bang
Sung-Il
SI
Jin
Young-Ho
YH
D000818:Animals; D017487:Ankyrins; D002104:Cadmium / Q000378:metabolism; D002120:Calcium Channel Agonists / Q000494:pharmacology; D002121:Calcium Channel Blockers / Q000494:pharmacology; D015220:Calcium Channels / Q000378:metabolism*; D002211:Capsaicin / Q000494:pharmacology; D002478:Cells, Cultured; D019706:Excitatory Postsynaptic Potentials / Q000187:drug effects; D018698:Glutamic Acid / Q000378:metabolism*; D017879:Isothiocyanates / Q000494:pharmacology; D008297:Male; D009474:Neurons / Q000187:drug effects / Q000378:metabolism*; D018408:Patch-Clamp Techniques; D051381:Rats; D017207:Rats, Sprague-Dawley; D012430:Ruthenium Red / Q000494:pharmacology; D017552:Solitary Nucleus / Q000187:drug effects / Q000378:metabolism*; D000074025:TRPA1 Cation Channel; D050052:TRPC Cation Channels; D013779:Tetrodotoxin / Q000494:pharmacology
D017487:Ankyrins; D002120:Calcium Channel Agonists; D002121:Calcium Channel Blockers; D015220:Calcium Channels; D017879:Isothiocyanates; D000074025:TRPA1 Cation Channel; D050052:TRPC Cation Channels; C480976:Trpa1 protein, rat; D002104:Cadmium; D012430:Ruthenium Red; D018698:Glutamic Acid; D013779:Tetrodotoxin; C004471:allyl isothiocyanate; D002211:Capsaicin