Glycolipids injected into the skin are presented to NKT cells in the draining lymph node independently of migratory skin dendritic cells.
APCs, such as dendritic cells (DC), can present glycolipid Ags on CD1d molecules to NKT cells. This interaction activates DC and NKT cells, leading to release of cytokines and enhanced T cell responses. Thus, glycolipid Ags are currently being tested as adjuvants for immunotherapy. We were interested in the interaction of murine skin DC with NKT cells in skin-draining lymph nodes. We observed that all skin DC subsets expressed CD1d upon migration to the lymph nodes. Moreover, skin DC were able to present the synthetic glycolipid Ag alpha-galactosylceramide (alpha-GalCer) to the NKT cell hybridoma DN32.D3. Intradermally injected alpha-GalCer was presented by migratory skin DC and lymph node DC to NKT hybridoma cells in vitro. When we injected alpha-GalCer intradermally into the skin, the numbers of various leukocyte subsets in the draining lymph nodes did not change significantly. However, T and B cells as well as NKT cells up-regulated the activation marker CD69. Coapplication of alpha-GalCer with the tumor model Ag OVA induced strong cytolytic CD8(+) T cell function that could inhibit the growth of B16 melanoma cells expressing OVA. However, mice that were devoid of migratory skin DC developed similar cytotoxic immune responses after intradermal immunization, indicating that skin DC are not required for the adjuvant properties of NKT cell activation and Ag presentation by this immunization route. In conclusion, migratory skin DC are able to interact with NKT cells; however, intradermally applied glycolipids are presented predominantly by lymph node DC to NKT cells.
Tripp
Christoph H
CH
Department of Dermatology and Venerology, Innsbruck Medical University, Innsbruck, Austria.
Sparber
Florian
F
Hermans
Ian F
IF
Romani
Nikolaus
N
Stoitzner
Patrizia
P
D000818:Animals; D055610:Antigens, CD1d / Q000276:immunology; D002460:Cell Line; D002465:Cell Movement / Q000276:immunology; D004195:Disease Models, Animal; D018450:Disease Progression; D005699:Galactosylceramides / Q000737:chemistry; D006017:Glycolipids / Q000737:chemistry / Q000276:immunology*; D007801:Langerhans Cells / Q000166:cytology / Q000276:immunology; D008198:Lymph Nodes / Q000276:immunology*; D051379:Mice; D008810:Mice, Inbred C57BL; D055611:Natural Killer T-Cells / Q000276:immunology*; D009368:Neoplasm Transplantation; D009369:Neoplasms / Q000276:immunology / Q000473:pathology; D012867:Skin / Q000166:cytology / Q000276:immunology*; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology; D046509:Tissue Culture Techniques
D055610:Antigens, CD1d; D005699:Galactosylceramides; D006017:Glycolipids; C493154:alpha-galactosylceramide