Biochemical characterization of haloalkane dehalogenases DrbA and DmbC, Representatives of a Novel Subfamily.
This study focuses on two representatives of experimentally uncharacterized haloalkane dehalogenases from the subfamily HLD-III. We report biochemical characterization of the expression products of haloalkane dehalogenase genes drbA from Rhodopirellula baltica SH1 and dmbC from Mycobacterium bovis 5033/66. The DrbA and DmbC enzymes show highly oligomeric structures and very low activities with typical substrates of haloalkane dehalogenases.
Jesenská
Andrea
A
Institute of Experimental Biology and National Center for Biomolecular Research, Masaryk University, Brno, Czech Republic.
Monincová
Marta
M
Koudeláková
Tána
T
Hasan
Khomaini
K
Chaloupková
Radka
R
Prokop
Zbynek
Z
Geerlof
Arie
A
Damborsky
Jirí
J
D001419:Bacteria / Q000201:enzymology*; D002942:Circular Dichroism; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D006867:Hydrolases / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*; D008969:Molecular Sequence Data; D009163:Mycobacterium bovis / Q000201:enzymology; D055503:Protein Multimerization; D017434:Protein Structure, Tertiary; D017422:Sequence Analysis, DNA; D013379:Substrate Specificity
D004269:DNA, Bacterial; D006867:Hydrolases; C046137:haloalkane dehalogenase