title: Autologous bone marrow cell transplantation increases leg perfusion and reduces amputations in patients with advanced critical limb ischemia due to peripheral artery disease.
abstract: Bone marrow cell transplantation has been shown to induce angiogenesis and thus improve ischemic artery disease. This study evaluates the effects of intramuscular bone marrow cell transplantation in patients with limb-threatening critical limb ischemia with a very high risk for major amputation. After failed or impossible operative and/or interventional revascularization and after unsuccessful maximum conservative therapy, 51 patients with impending major amputation due to severe critical limb ischemia had autologous bone marrow cells (BMC) transplanted into the ischemic leg. Patients 1-12 received Ficoll-isolated bone marrow mononuclear cells (total cell number 1.1 +/- 1.1 x 10(9)), patients 13-51 received point of care isolated bone marrow total nucleated cells (3.0 +/- 1.7 x 10(9)). Limb salvage was 59% at 6 months and 53% at last follow-up (mean 411 +/- 261 days, range 175-1186). Perfusion measured with ankle-brachial index (ABI) and transcutaneous oxygen tension (tcpO(2)) at baseline and after 6 months increased in patients with consecutive limb salvage (ABI 0.33 +/- 0.18 to 0.46 +/- 0.15, tcpO(2) 12 +/- 12 to 25 +/- 15 mmHg) and did not change in patients eventually undergoing major amputation. No difference in clinical outcome between the isolation methods were seen. Clinically most important, patients with limb salvage improved from a mean Rutherford category of 4.9 at baseline to 3.3 at 6 months (p = 0.0001). Analgesics consumption was reduced by 62%. Total walking distance improved in nonamputees from zero to 40 m. Three severe periprocedural adverse events resolved without sequelae, and no unexpected long-term adverse events occurred. In no-option patients with end-stage critical limb ischemia due to peripheral artery disease, bone marrow cell transplantation is a safe procedure that can improve leg perfusion sufficiently to reduce major amputations and permit durable limb salvage.
authors.affiliation: Department of Medicine, Franziskuskrankenhaus, Berlin Vascular Center, Berlin, Germany. amann@franziskus-berlin.de
authors.forename: Berthold
authors.initials: B
authors.lastname: Amann
authors.forename: Claas
authors.initials: C
authors.lastname: Luedemann
authors.forename: Richard
authors.initials: R
authors.lastname: Ratei
authors.forename: J André
authors.initials: JA
authors.lastname: Schmidt-Lucke
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000671:Amputation, Surgical; D000792:Angiography; D055109:Ankle Brachial Index; D016026:Bone Marrow Transplantation; D005080:Exercise Test; D005260:Female; D006801:Humans; D007511:Ischemia / Q000150:complications* / Q000503:physiopathology / Q000601:surgery*; D007866:Leg / Q000098:blood supply* / Q000601:surgery*; D008297:Male; D008875:Middle Aged; D010477:Perfusion; D016491:Peripheral Vascular Diseases / Q000150:complications* / Q000503:physiopathology / Q000601:surgery; D016019:Survival Analysis; D014182:Transplantation, Autologous; D016896:Treatment Outcome; D016138:Walking; D014945:Wound Healing