[The anti-proliferation and anti-migration dual effects of aloe-emodin on KB cells and its mechanism].
OBJECTIVE
To investigate the anti-proliferation and anti-migration dual effects of aloe-emodin on KB cells and its mechanisms.


METHODS
KB cells were treated with 2.5, 5, 10, 20 and 40 micromol/L aloe-emodin. Crystal violet assay was used to determine the long-term growth inhibition of aloe-emodin on human oral cancer KB cells. Scratch wound-healing motility assay was used to measure the antimigration effect The protein kinase C alpha and c-myc expression changes in protein levels were detected by Western blotting.


RESULTS
A durable cell growth inhibitory effect of aloe-emodin on KB cells was found. Treatment of aloe-emodin resulted in the inhibition of cell migration. The protein kinase C alpha and c-myc in protein levels were decreased upon treatment with aloe-emodin compared with controls.


CONCLUSIONS
The anti-proliferation and anti-migration effects of aloe-emodin on KB cells are associated with the suppression of protein kinase C pathway.
Xiao
Bing-Xiu
BX
Department of Biochemistry, Ningbo University School of Medicine, Ningbo Zhejiang 315211, China,
Guo
Junming
J
D000880:Anthraquinones / Q000494:pharmacology*; D002465:Cell Movement / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects*; D016259:Genes, myc; D006801:Humans; D007624:KB Cells; D011493:Protein Kinase C / Q000378:metabolism
D000880:Anthraquinones; C518327:aloe emodin; D011493:Protein Kinase C