title: Analysis of protein-protein interactions by using droplet-based microfluidics.
abstract: Every little drop: The K(D) values of angiogenin (ANG) interactions as shown by FRET analysis of thousands of pL-sized droplets agree with data from bulk-fluorescence polarization measurements. Importantly, the use of fluorophores does not affect the activity of ANG or the binding of anti-ANG antibodies to ANG. Such an experimental platform could be applied to the high-throughput analysis of protein-protein interactions.
authors.affiliation: Department of Chemistry, Imperial College London, Exhibition Road, London, UK.
authors.forename: Monpichar
authors.initials: M
authors.lastname: Srisa-Art
authors.forename: Dong-Ku
authors.initials: DK
authors.lastname: Kang
authors.forename: Jongin
authors.initials: J
authors.lastname: Hong
authors.forename: Hyun
authors.initials: H
authors.lastname: Park
authors.forename: Robin J
authors.initials: RJ
authors.lastname: Leatherbarrow
authors.forename: Joshua B
authors.initials: JB
authors.lastname: Edel
authors.forename: Soo-Ik
authors.initials: SI
authors.lastname: Chang
authors.forename: Andrew J
authors.initials: AJ
authors.lastname: deMello
mesh_terms: D000906:Antibodies / Q000276:immunology; D002478:Cells, Cultured; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods*; D005456:Fluorescent Dyes / Q000737:chemistry; D006801:Humans; D007700:Kinetics; D044085:Microfluidics / Q000295:instrumentation / Q000379:methods*; D025941:Protein Interaction Mapping / Q000379:methods*; D012259:Ribonuclease, Pancreatic / Q000032:analysis* / Q000276:immunology / Q000378:metabolism; D013050:Spectrometry, Fluorescence; D013816:Thermodynamics
chemical_list: D000906:Antibodies; D005456:Fluorescent Dyes; C046366:angiogenin; D012259:Ribonuclease, Pancreatic