title: A novel transcriptomic approach to identify candidate genes for grain quality traits in wheat.
abstract: A novel methodology is described in which transcriptomics is combined with the measurement of bread-making quality and other agronomic traits for wheat genotypes grown in different environments (wet and cool or hot and dry conditions) to identify transcripts associated with these traits. Seven doubled haploid lines from the Spark x Rialto mapping population were selected to be matched for development and known alleles affecting quality. These were grown in polytunnels with different environments applied 14 days post-anthesis, and the whole experiment was repeated over 2 years. Transcriptomics using the wheat Affymetrix chip was carried out on whole caryopsis samples at two stages during grain filling. Transcript abundance was correlated with the traits for approximately 400 transcripts. About 30 of these were selected as being of most interest, and markers were derived from them and mapped using the population. Expression was identified as being under cis control for 11 of these and under trans control for 18. These transcripts are candidates for involvement in the biological processes which underlie genotypic variation in these traits.
authors.affiliation: Rothamsted Research, Harpenden, Hertfordshire, UK.
authors.forename: Yongfang
authors.initials: Y
authors.lastname: Wan
authors.forename: Claudia
authors.initials: C
authors.lastname: Underwood
authors.forename: Geraldine
authors.initials: G
authors.lastname: Toole
authors.forename: Peter
authors.initials: P
authors.lastname: Skeggs
authors.forename: Tong
authors.initials: T
authors.lastname: Zhu
authors.forename: Michelle
authors.initials: M
authors.lastname: Leverington
authors.forename: Simon
authors.initials: S
authors.lastname: Griffiths
authors.forename: Tim
authors.initials: T
authors.lastname: Wheeler
authors.forename: Mike
authors.initials: M
authors.lastname: Gooding
authors.forename: Rebecca
authors.initials: R
authors.lastname: Poole
authors.forename: Keith J
authors.initials: KJ
authors.lastname: Edwards
authors.forename: Salvador
authors.initials: S
authors.lastname: Gezan
authors.forename: Sue
authors.initials: S
authors.lastname: Welham
authors.forename: John
authors.initials: J
authors.lastname: Snape
authors.forename: E N Clare
authors.initials: EN
authors.lastname: Mills
authors.forename: Rowan A C
authors.initials: RA
authors.lastname: Mitchell
authors.forename: Peter R
authors.initials: PR
authors.lastname: Shewry
mesh_terms: D018556:Crops, Agricultural / Q000235:genetics; D003433:Crosses, Genetic; D020869:Gene Expression Profiling / Q000379:methods*; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D005838:Genotype; D006238:Haploidy; D019655:Quantitative Trait, Heritable; D018749:RNA, Plant / Q000235:genetics; D012639:Seeds / Q000235:genetics*; D014908:Triticum / Q000235:genetics*
chemical_list: D018749:RNA, Plant