Human single-donor composite skin substitutes based on collagen and polycaprolactone copolymer.
The development and characterization of an enhanced composite skin substitute based on collagen and poly(epsilon-caprolactone) are reported. Considering the features of excellent biocompatibility, easy-manipulated property and exempt from cross-linking related toxicity observed in the 1:20 biocomposites, skin substitutes were developed by seeding human single-donor keratinocytes and fibroblasts alone on both sides of the 1:20 biocomposite to allow for separation of two cell types and preserving cell signals transmission via micro-pores with a porosity of 28.8 +/- 16.1 microm. The bi-layered skin substitute exhibited both differentiated epidermis and fibrous dermis in vitro. Less Keratinocyte Growth Factor production was measured in the co-cultured skin model compared to fibroblast alone condition indicating a favorable microenvironment for epidermal homeostasis. Moreover, fast wound closure, epidermal differentiation, and abundant dermal collagen deposition were observed in composite skin in vivo. In summary, the beneficial characteristics of the new skin substitutes exploited the potential for pharmaceutical screening and clinical application.
Dai
Niann-Tzyy
NT
Division of Plastic and Reconstructive Surgery, Department of Surgery, Tri-Service General Hospital, National Defense Medical Center, Taiwan, ROC. niantzyy_dai@hotmail.com
Yeh
Ming-Kung
MK
Chiang
Chiao-Hsi
CH
Chen
Ke-Chi
KC
Liu
Tsung-Hsun
TH
Feng
An-Chieh
AC
Chao
Li-Lien
LL
Shih
Chien-Ming
CM
Sytwu
Huey-Kang
HK
Chen
Shao-Liang
SL
Chen
Tim-Mo
TM
Adams
E F
EF
D000818:Animals; D018920:Coculture Techniques; D003094:Collagen / Q000737:chemistry*; D051523:Fibroblast Growth Factor 7 / Q000096:biosynthesis; D005347:Fibroblasts / Q000378:metabolism / Q000502:physiology; D006801:Humans; D015603:Keratinocytes / Q000378:metabolism / Q000502:physiology; D008422:Materials Testing; D051379:Mice; D008819:Mice, Nude; D011091:Polyesters / Q000737:chemistry*; D016062:Porosity; D017768:Skin, Artificial; D014019:Tissue Donors
D011091:Polyesters; D051523:Fibroblast Growth Factor 7; C016240:polycaprolactone; D003094:Collagen