Contrast and depth enhancement in two-photon microscopy of human skin ex vivo by use of optical clearing agents.
We investigate the application of hyperosmotic optical clearing agents to improve the image contrast and penetration depth in two-photon microscopy of human dermis ex vivo. We show that the agents glycerol, propylene glycol, and glucose all convey significant improvements and we provide results on their dynamic behaviour and the reversibility of the effect. At suitable concentrations, such agents have the potential to be compatible with living tissue and may possibly enhance in-vivo deep-tissue imaging.
Cicchi
R
R
Sampson
D
D
Massi
D
D
Pavone
F
F