title: Generation of transgenic non-human primates with germline transmission.
abstract: The common marmoset (Callithrix jacchus) is increasingly attractive for use as a non-human primate animal model in biomedical research. It has a relatively high reproduction rate for a primate, making it potentially suitable for transgenic modification. Although several attempts have been made to produce non-human transgenic primates, transgene expression in the somatic tissues of live infants has not been demonstrated by objective analyses such as polymerase chain reaction with reverse transcription or western blots. Here we show that the injection of a self-inactivating lentiviral vector in sucrose solution into marmoset embryos results in transgenic common marmosets that expressed the transgene in several organs. Notably, we achieved germline transmission of the transgene, and the transgenic offspring developed normally. The successful creation of transgenic marmosets provides a new animal model for human disease that has the great advantage of a close genetic relationship with humans. This model will be valuable to many fields of biomedical research.
authors.affiliation: Central Institute for Experimental Animals, 1430 Nogawa, Miyamae-ku, Kawasaki, Kanagawa 216-0001, Japan. esasaki@ciea.or.jp
authors.forename: Erika
authors.initials: E
authors.lastname: Sasaki
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Suemizu
authors.forename: Akiko
authors.initials: A
authors.lastname: Shimada
authors.forename: Kisaburo
authors.initials: K
authors.lastname: Hanazawa
authors.forename: Ryo
authors.initials: R
authors.lastname: Oiwa
authors.forename: Michiko
authors.initials: M
authors.lastname: Kamioka
authors.forename: Ikuo
authors.initials: I
authors.lastname: Tomioka
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Sotomaru
authors.forename: Reiko
authors.initials: R
authors.lastname: Hirakawa
authors.forename: Tomoo
authors.initials: T
authors.lastname: Eto
authors.forename: Seiji
authors.initials: S
authors.lastname: Shiozawa
authors.forename: Takuji
authors.initials: T
authors.lastname: Maeda
authors.forename: Mamoru
authors.initials: M
authors.lastname: Ito
authors.forename: Ryoji
authors.initials: R
authors.lastname: Ito
authors.forename: Chika
authors.initials: C
authors.lastname: Kito
authors.forename: Chie
authors.initials: C
authors.lastname: Yagihashi
authors.forename: Kenji
authors.initials: K
authors.lastname: Kawai
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Miyoshi
authors.forename: Yoshikuni
authors.initials: Y
authors.lastname: Tanioka
authors.forename: Norikazu
authors.initials: N
authors.lastname: Tamaoki
authors.forename: Sonoko
authors.initials: S
authors.lastname: Habu
authors.forename: Hideyuki
authors.initials: H
authors.lastname: Okano
authors.forename: Tatsuji
authors.initials: T
authors.lastname: Nomura
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified / Q000235:genetics*; D000831:Animals, Newborn; D002144:Callithrix / Q000196:embryology / Q000235:genetics*; D004195:Disease Models, Animal; D020869:Gene Expression Profiling; D005854:Germ Cells / Q000378:metabolism*; D049452:Green Fluorescent Proteins / Q000235:genetics; D040941:Heredity / Q000235:genetics*; D006801:Humans; D014158:Transcription, Genetic; D019076:Transgenes / Q000235:genetics*
chemical_list: C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins