Direct protein detection with a nano-interdigitated array gate MOSFET.
A new protein sensor is demonstrated by replacing the gate of a metal oxide semiconductor field effect transistor (MOSFET) with a nano-interdigitated array (nIDA). The sensor is able to detect the binding reaction of a typical antibody Ixodes ricinus immunosuppressor (anti-Iris) protein at a concentration lower than 1 ng/ml. The sensor exhibits a high selectivity and reproducible specific detection. We provide a simple model that describes the behavior of the sensor and explains the origin of its high sensitivity. The simulated and experimental results indicate that the drain current of nIDA-gate MOSFET sensor is significantly increased with the successive binding of the thiol layer, Iris and anti-Iris protein layers. It is found that the sensor detection limit can be improved by well optimizing the geometrical parameters of nIDA-gate MOSFET. This nanobiosensor, with real-time and label-free capabilities, can easily be used for the detection of other proteins, DNA, virus and cancer markers. Moreover, an on-chip associated electronics nearby the sensor can be integrated since its fabrication is compatible with complementary metal oxide semiconductor (CMOS) technology.
Tang
Xiaohui
X
Microelectronics Laboratory (DICE) and CERMIN, Université catholique de Louvain (UCL), Place du Levant 3, 1348 Louvain-la-Neuve, Belgium. Xiaohui.tang@uclouvain.be
Jonas
Alain M
AM
Nysten
Bernard
B
Demoustier-Champagne
Sophie
S
Blondeau
Franoise
F
Prévot
Pierre-Paul
PP
Pampin
Rémi
R
Godfroid
Edmond
E
Iñiguez
Benjamin
B
Colinge
Jean-Pierre
JP
Raskin
Jean-Pierre
JP
Flandre
Denis
D
Bayot
Vincent
V
D015374:Biosensing Techniques / Q000295:instrumentation*; D017076:Computer-Aided Design; D004563:Electrochemistry / Q000295:instrumentation*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D036103:Nanotechnology / Q000295:instrumentation*; D040081:Protein Array Analysis / Q000295:instrumentation*; D025941:Protein Interaction Mapping / Q000295:instrumentation*; D012815:Signal Processing, Computer-Assisted / Q000295:instrumentation*; D014159:Transducers; D014173:Transistors, Electronic