Animal cloning by somatic cell nuclear transfer.
Animal cloning is becoming increasingly useful for its applications in biological inquiry and for its potential use in pharmaceutical, medical, and agricultural fields. Due to the complexity of the numerous steps required in reconstructing oocytes by nuclear transfer, detailed protocols are required to minimize the developmental damages inflicted during these manipulations and to standardize procedures across laboratories. Moreover, because oogenesis and early embryogenesis differ widely among mammalian species, it is essential that protocols be adapted according to each species concerned. Our objective here is to detail the protocols that have been most successful in producing laboratory and domestic animal clones.
Smith
Lawrence C
LC
Animal Reproduction Research Centre, Faculty of Veterinary Medicine, University of Montreal, St-Hyacinthe, Québec, Canada.
Yoo
Jae-Gyu
JG
D000818:Animals; D002417:Cattle; D019976:Cloning, Organism; D003470:Culture Media; D051379:Mice; D053652:Nuclear Transfer Techniques; D009865:Oocytes; D013552:Swine
D003470:Culture Media