Sequence and structural analysis of artemin based on ferritin: a comparative study.
Artemia cysts can tolerate extreme environments, partly due to a heat-stable protein called artemin. According to previous studies, artemin shares structural similarity with ferritins. Actually, there is still no strong structural information about artemin three-dimensional (3-D) structure. In this research, the artemin encoding gene from Artemia urmiana was cloned and sequenced. A reliable 3-D model of artemin was initially built using ferritin as template and refined using Molecular Dynamic (MD) Simulation. It is interesting that the proposed model, confirmed by circular dichroism (CD), shows significant differences in secondary structure contents with ferritin. Three conserved regions (ferroxidase center, iron nucleation center and 3-fold channel) in ferritins, cooperating in iron-interaction, have been substantially changed in artemin. Analysis of C-terminal region of the model revealed its major role in preventing artemin from iron-binding due to some suitable interactions. Finally, it is concluded that significant differences between artemin and ferritin, both in conserved regions related to iron-interaction and three-dimensional structure, can justify their functional differences.
Rasti
Behnam
B
Department of Biology, Faculty of Science, University of Guilan, Rasht, Iran.
Shahangian
S Shirin
SS
Sajedi
Reza H
RH
Taghdir
Majid
M
Hasannia
Sadegh
S
Ranjbar
Bijan
B
D000595:Amino Acid Sequence; D000818:Animals; D001156:Artemia / Q000737:chemistry* / Q000235:genetics*; D060829:Arthropod Proteins; D001483:Base Sequence; D002570:Ceruloplasmin / Q000737:chemistry / Q000235:genetics; D002942:Circular Dichroism; D003001:Cloning, Molecular; D017124:Conserved Sequence; D017931:DNA Primers / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics; D005293:Ferritins / Q000737:chemistry* / Q000235:genetics*; D007447:Invertebrate Hormones / Q000737:chemistry* / Q000235:genetics*; D007501:Iron / Q000737:chemistry; D033862:Iron-Binding Proteins; D008958:Models, Molecular; D008969:Molecular Sequence Data; D011487:Protein Conformation; D017433:Protein Structure, Secondary; D016601:RNA-Binding Proteins / Q000737:chemistry* / Q000235:genetics*; D017386:Sequence Homology, Amino Acid; D013816:Thermodynamics
D060829:Arthropod Proteins; D017931:DNA Primers; D018076:DNA, Complementary; D007447:Invertebrate Hormones; D033862:Iron-Binding Proteins; D016601:RNA-Binding Proteins; C490888:artemin protein, brine shrimp; D005293:Ferritins; D007501:Iron; D002570:Ceruloplasmin