The cryoprotective effect of trehalose supplementation on boar spermatozoa quality.
In order to improve boar sperm quality during frozen-thawed process, the influence of the presence of trehalose on success of cryopreservation of boar sperm were investigated. We evaluated freeze-thawing tolerance of boar spermatozoa in a base cooling extender with the addition of different trehalose concentrations (0, 25, 50, 100 and 200 mm), and try to determine the optimum concentration of trehalose. We chose sperm motility, mitochondrial activity, acrosome integrity and membrane integrity as parameters to evaluate cryopreservation capacity of boar spermatozoa. We obtained the best results for 100 mm trehalose-supplemented extenders, with values of 49.89% for motility, 44.69% for mitochondrial activity, 66.52% for acrosome integrity and 44.61% for membrane integrity, while freeze-thawing tolerance diminished significantly for 200 . The synergic effect of trehalose and glycerol resulted in better cryosurvival of boar spermatozoa than that of a single cryoprotectant. In conclusion, when trehalose-supplementation was added up to 100 mm, trehalose confers a greater cryoprotective capacity to the extender, and the sperm motility, mitochondrial activity, membrane integrity and acrosome integrity parameters were significantly improved during frozen-thawed process.
Hu
J-H
JH
College of Animal Science and Technology, Northwest A & F University, Yangling, Shaanxi, China.
Li
Q-W
QW
Jiang
Z-L
ZL
Yang
H
H
Zhang
S-S
SS
Zhao
H-W
HW
D000177:Acrosome / Q000648:ultrastructure; D000818:Animals; D002462:Cell Membrane / Q000648:ultrastructure; D015925:Cryopreservation / Q000379:methods / Q000662:veterinary*; D003451:Cryoprotective Agents / Q000008:administration & dosage*; D004357:Drug Synergism; D005990:Glycerol / Q000008:administration & dosage; D008297:Male; D008856:Microscopy, Fluorescence; D008928:Mitochondria / Q000502:physiology; D012662:Semen Preservation / Q000379:methods / Q000662:veterinary*; D013081:Sperm Motility; D013094:Spermatozoa / Q000502:physiology* / Q000648:ultrastructure; D013552:Swine; D014199:Trehalose / Q000008:administration & dosage*
D003451:Cryoprotective Agents; D014199:Trehalose; D005990:Glycerol