title: Response surface methodology for optimization of medium components in submerged culture of Aspergillus flavus for enhanced heparinase production.
abstract: OBJECTIVE
  Aim of the study was to develop a medium for optimal heparinase production with a strain of Aspergillus flavus (MTCC-8654) by using a multidimensional statistical approach.
  
  
  RESULTS
  Statistical optimization of intracellular heparinase production by A. flavus, a new isolate, was investigated. Plackett-Burman design was used to evaluate the affect of medium constituents on heparinase yield. The experimental results showed that the production of heparinase was dependent upon heparin, the inducer; chitin, structurally similar to heparin and NH(4)NO(3,) the nitrogen source. A central composite design was applied to derive a statistical model for optimizing the composition of the fermentation medium for the production of heparinase enzyme. The optimum fermentation medium consisted of (g l(-1)) Mannitol, 8.0; NH(4)NO(3), 2.5; K(2)HPO(4), 2.5; Na(2)HPO(4), 2.5; MgSO(4).7H(2)O, 0.5; Chitin, 17.1; Heparin, 0.6; trace salt solution (NaMoO(4).2H(2)O, CoCl(2).6H(2)O, CuSO(4).5H(2)O, FeSO(4).7H(2)O, CaCl(2)), 10(-4) mol l(-1).
  
  
  CONCLUSIONS
  A 2.37-fold increase in heparinase production was achieved in economic and effective manner by the application of statistical designs in medium optimization.
  
  
  CONCLUSIONS
  Heparinase production was doubled by statistical optimization in a cost-effective manner. This heparinase can find application in pharmaceutical industry and for the generation of low-molecular-weight heparins, active as antithrombotic and antitumour agents.
authors.affiliation: Fermentation Technology Division, Central Drug Research Institute, Lucknow, UP, India.
authors.forename: J
authors.initials: J
authors.lastname: Banga
authors.forename: C K M
authors.initials: CK
authors.lastname: Tripathi
mesh_terms: D001231:Aspergillus flavus / Q000201:enzymology* / Q000254:growth & development*; D002686:Chitin / Q000378:metabolism; D003470:Culture Media / Q000737:chemistry; D003627:Data Interpretation, Statistical; D006493:Heparin / Q000378:metabolism; D019763:Heparin Lyase / Q000096:biosynthesis*; D009566:Nitrates / Q000378:metabolism
chemical_list: D003470:Culture Media; D009566:Nitrates; D002686:Chitin; D006493:Heparin; D019763:Heparin Lyase; C006568:ammonium nitrate