title: Protein microarray analysis of nasal polyps from aspirin-sensitive and aspirin-tolerant patients with chronic rhinosinusitis.
abstract: BACKGROUND
  The purpose of this study was to apply protein microarray technology to the study of sinonasal tissue and to identify differential protein expression in nasal polyps from aspirin-sensitive (AS) versus aspirin-tolerant (AT) patients with chronic rhinosinusitis (CRS) and CRS with nasal polyps (CRSwNPs).
  
  
  METHODS
  Nasal polyp specimens were prospectively obtained from two groups of patients with CRSwNP. The test group (AS) consisted of five patients that were diagnosed with CRSwNP and intolerance to aspirin based on medical history and physical exam. The control group (AT) consisted of four AT patients with CRSwNP. Protein was extracted and labeled from harvested polyps and the Sigma Panorama Antibody Microarray-Cell Signaling Kit was used to identify differences in protein expression between the two polyp groups. Western blot analysis was used to validate the results of the protein microarray.
  
  
  RESULTS
  The protein microarray showed a greater than twofold change in expression of both beta-adaptin and heat shock protein 70 (HSP70). Western blot analysis confirmed up-regulation of beta-adaptin and HSP70 in nasal polyp tissue from AS patients.
  
  
  CONCLUSIONS
  Pooled samples of AS and AT nasal polyps evaluated by protein microarray show distinct protein expression profiles in the stress response and receptor-mediated endocytosis pathways. This study establishes the successful application of protein microarray technology to study nasal polyposis, which in turn can be validated by Western blot analysis.
authors.affiliation: Department of Otolaryngology-Head and Neck Surgery, University of Colorado Denver, Denver, Colorado, USA.
authors.forename: Kelly A
authors.initials: KA
authors.lastname: Zander
authors.forename: Milene T
authors.initials: MT
authors.lastname: Saavedra
authors.forename: James
authors.initials: J
authors.lastname: West
authors.forename: Victor
authors.initials: V
authors.lastname: Scapa
authors.forename: Linda
authors.initials: L
authors.lastname: Sanders
authors.forename: Todd T
authors.initials: TT
authors.lastname: Kingdom
mesh_terms: D033966:Adaptor Protein Complex beta Subunits / Q000032:analysis / Q000235:genetics; D000328:Adult; D000368:Aged; D001241:Aspirin / Q000494:pharmacology*; D053181:Caspase 8 / Q000032:analysis; D053453:Caspase 9 / Q000032:analysis; D002908:Chronic Disease; D004361:Drug Tolerance; D005260:Female; D018840:HSP70 Heat-Shock Proteins / Q000032:analysis / Q000235:genetics; D006801:Humans; D008297:Male; D008875:Middle Aged; D009298:Nasal Polyps / Q000378:metabolism*; D040081:Protein Array Analysis / Q000379:methods*; D012220:Rhinitis / Q000378:metabolism*; D012852:Sinusitis / Q000378:metabolism*
chemical_list: D033966:Adaptor Protein Complex beta Subunits; D018840:HSP70 Heat-Shock Proteins; D053181:Caspase 8; D053453:Caspase 9; D001241:Aspirin