Gene expression analysis of human endometrial endothelial cells exposed to Bisphenol A.
Bisphenol A (BPA) can affect reproductive tissues in several species. Recently, treatment of human endometrial endothelial cells (HEECs) with 100 microM BPA decreased their proliferation and viability. In the present study, 50 microM BPA decreased HEEC proliferation, and microarray analyses of five HEEC cultures revealed that BPA affected biological processes associated with proliferation. Expression of three of the most differentially expressed genes identified in the gene array analysis, SPBC25, SGOL2 and CDCA8, was verified by real-time qRT-PCR in five HEEC cultures obtained from women in the proliferative phase and in five cultures obtained from women in the secretory phase of the menstrual cycle after treatment with BPA. This study supports our previous findings of decreased cell proliferation and increased cell death in response to BPA, and may offer important clues to the mechanisms of action of BPA. Furthermore, the study implies a possible impact of BPA on female fertility functions.
Bredhult
Carolina
C
Department of Women's and Children's Health, Uppsala University, SE-751 85 Uppsala, Sweden. Carolina.Bredhult@kbh.uu.se
Sahlin
Lena
L
Olovsson
Matts
M
D001559:Benzhydryl Compounds; D018797:Cell Cycle Proteins / Q000235:genetics; D049109:Cell Proliferation / Q000187:drug effects; D016000:Cluster Analysis; D052244:Endocrine Disruptors / Q000633:toxicity*; D004717:Endometrium / Q000098:blood supply*; D042783:Endothelial Cells / Q000187:drug effects* / Q000378:metabolism; D005260:Female; D005498:Follicular Phase; D020869:Gene Expression Profiling / Q000379:methods; D005786:Gene Expression Regulation / Q000187:drug effects*; D006801:Humans; D008183:Luteal Phase; D020411:Oligonucleotide Array Sequence Analysis; D010636:Phenols / Q000633:toxicity*; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D000098826:Bisphenol A Compounds
D001559:Benzhydryl Compounds; D018797:Cell Cycle Proteins; D052244:Endocrine Disruptors; D010636:Phenols; D012333:RNA, Messenger; C490157:CDCA8 protein, human; C510590:SGO2 protein, human; C006780:bisphenol A; D000098826:Bisphenol A Compounds