The combination of urine DD3(PCA3) mRNA and PSA mRNA as molecular markers of prostate cancer.
The identification of reliable molecular biomarkers in prostate cancer early diagnosis is clinically desirable. We quantitatively detected prostate cancer specifically overexpressed genes, DD3 and PSA, in urine sediments of men suffering from prostate cancer or benign prostate hyperplasia, after prostatic massage. As both genes are exclusively expressed in androgen receptor expressing human prostate carcinoma cell lines, we further investigated the possible effect of androgens on PSA and DD3 gene expression. DD3 and PSA mRNA levels were measured by real-time polymerase chain reaction. The combined markers test had a sensitivity of 80.2% and a specificity of 100%. Both gene transcripts were significantly upregulated by androgens. Results indicated the clinical usefulness of the combination of DD3 and PSA as molecular markers in the early diagnosis of prostate cancer and the need of combining as many as possible analytical data with the clinical and demographic ones to achieve the maximum level of diagnostic accuracy.
Mearini
Ettore
E
University of Perugia, Department of Medical-Surgical Specialties and Public Health, Division of Urology and Andrology, Perugia, Italy. talesa@unipg.it
Antognelli
Cinzia
C
Del Buono
Chiara
C
Cochetti
Giovanni
G
Giannantoni
Antonella
A
Nardelli
Emanuela
E
Talesa
Vincenzo N
VN
D000368:Aged; D000369:Aged, 80 and over; D000951:Antigens, Neoplasm / Q000652:urine*; D014408:Biomarkers, Tumor / Q000652:urine*; D006801:Humans; D008297:Male; D008875:Middle Aged; D017430:Prostate-Specific Antigen / Q000652:urine*; D011470:Prostatic Hyperplasia / Q000235:genetics / Q000652:urine; D011471:Prostatic Neoplasms / Q000235:genetics* / Q000652:urine; D012333:RNA, Messenger / Q000652:urine*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013739:Testosterone
D000951:Antigens, Neoplasm; D014408:Biomarkers, Tumor; D012333:RNA, Messenger; C479341:prostate cancer antigen 3, human; D013739:Testosterone; D017430:Prostate-Specific Antigen