Influence of methionine upon the activity of antioxidative enzymes in the kidney of rats exposed to sodium fluoride.
The intensified or uncontrolled formation of reactive oxygen species leads to disturbances of numerous biochemical processes. Among the factors inducing intensified free radical processes, fluoride ions are listed, among others. One of the organs most exposed to the toxic activity of fluorides is the kidney. In the study presented here, the influence of fluorine upon the activity of selected antioxidant enzymes in rat kidney has been examined, as well as antioxidant properties of methionine during intoxication with sodium fluoride. The experiment was carried out on Wistar FL rats (adult females) that for 35 days were administered water, NaF, NaF with methionine (doses: 10 mg NaF/kg bw/day, 10 mg Met/kg bw/day) . The influence of administered NaF and Met upon the antioxidative system in kidney was examined by analyzing the activity of the most important antioxidative enzymes (SOD, CAT, GPX, GR, GST). The studies carried out confirmed the disadvantageous effect of NaF upon the antioxidative system in rats (decrease in activity of antioxidative enzymes). Methionine increased the activity of antioxidative enzymes, most efficiently that of GPX, GR, and GST.
Błaszczyk
Iwona
I
Department of Biochemistry, Silesian Medical University, 41-808, Zabrze, Poland. iblaszczyk@sum.edu.pl
Grucka-Mamczar
Ewa
E
Kasperczyk
Sławomir
S
Birkner
Ewa
E
D000818:Animals; D002374:Catalase / Q000378:metabolism; D004798:Enzymes / Q000378:metabolism*; D005260:Female; D016166:Free Radical Scavengers / Q000378:metabolism; D005982:Glutathione Transferase / Q000378:metabolism; D007668:Kidney / Q000187:drug effects*; D008715:Methionine / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D012969:Sodium Fluoride / Q000633:toxicity*; D013482:Superoxide Dismutase / Q000378:metabolism
D004798:Enzymes; D016166:Free Radical Scavengers; D012969:Sodium Fluoride; D008715:Methionine; D002374:Catalase; D013482:Superoxide Dismutase; D005982:Glutathione Transferase