Detection of breast micro-metastases in axillary lymph nodes by infrared micro-spectral imaging.
We report the ability of infrared micro-spectral imaging, coupled with completely unsupervised methods of multivariate statistical analysis, to accurately reproduce the histological architecture of axillary lymph nodes and detect metastatic breast cancer cells. The acquisition of spectral data from tissue embedded in paraffin provided spectra free of dispersive artefacts that may be observed for infrared microscopic measurements using a 'reflection/absorption' methodology. As a consequence, superior tissue classification and identification of cellular abnormality unattainable for deparaffinised tissue was achieved.
Bird
Benjamin
B
Laboratory for Spectral Diagnosis (LSpD), Department of Chemistry and Chemical Biology, Northeastern University, 360 Huntington Avenue, Boston, USA. benjamin_bird@hotmail.com
Bedrossian
Kristi
K
Laver
Nora
N
Miljković
Milos
M
Romeo
Melissa J
MJ
Diem
Max
M
D001943:Breast Neoplasms / Q000378:metabolism / Q000473:pathology*; D006801:Humans; D008207:Lymphatic Metastasis / Q000175:diagnosis* / Q000473:pathology; D008853:Microscopy; D015591:Microspectrophotometry; D013055:Spectrophotometry, Infrared; D013194:Staining and Labeling