Phage-displayed antibody fragments recognizing dengue 3 and dengue 4 viruses as tools for viral serotyping in sera from infected individuals.
The current study shows the usefulness of dengue-3- and dengue-4-specific phage-displayed antibody fragments as tools for viral detection and serotyping in sera from infected individuals. C6/36 HT cells were inoculated with acute-phase sera from patients, and supernatants were collected daily and analyzed by ELISA using phage-displayed antibody fragments as serotype-specific detector reagents. Serotyping of most samples was possible as early as two to three days postinoculation. Results were comparable with those obtained by indirect immunofluorescence assay but were obtained in a shorter period of time (<1 week). Phage-displayed antibody fragments were better tools for diagnosis and serotyping than their soluble counterparts. Our approach combines the advantages of viral isolation and ELISA techniques. These results could be the basis for the development of a high-throughput method for identifying dengue virus serotypes, which is crucial for the management and control of the disease.
Cabezas
Sheila
S
Department of Virology, PAHO/WHO Collaborating Center for the Study of Dengue and its Vector, "Pedro Kourí" Tropical Medicine Institute, Marianao, Havana, Cuba. sheila@ipk.sld.cu
Rojas
Gertrudis
G
Pavon
Alequis
A
Bernardo
Lidice
L
Castellanos
Yinet
Y
Alvarez
Mayling
M
Pupo
Maritza
M
Guillen
Gerardo
G
Guzman
Maria G
MG
D000810:Angola / Q000453:epidemiology; D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral / Q000276:immunology*; D000917:Antibody Formation; D003462:Cuba / Q000453:epidemiology; D003715:Dengue / Q000097:blood / Q000175:diagnosis* / Q000453:epidemiology / Q000276:immunology*; D003716:Dengue Virus / Q000145:classification / Q000276:immunology* / Q000302:isolation & purification; D004196:Disease Outbreaks; D019424:Dominica / Q000453:epidemiology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D006801:Humans; D010446:Peptide Fragments / Q000276:immunology; D012703:Serotyping; D010948:Viral Plaque Assay
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D010446:Peptide Fragments