title: Electrotaxis of lung cancer cells in a multiple-electric-field chip.
abstract: We report a microfluidic cell culture chip that was used for long-term electrotaxis study on a microscope. The cellular response under three different electric field strengths was studied in a single channel microfluidic chip. Electric field (EF) inside the microchamber was numerically simulated and compared to the measured value. Lung cancer cell lines with high and weak metastasis potential, CL1-5 and CL1-0, respectively, were used to demonstrate the function of the multi-field chip (MFC). The two cell lines exhibited greatly different response under the applied EF of E=74-375 mV/mm. CL1-5 cells migrated toward the anode while CL1-0 cells did not show obvious response. Under the applied EF, cell orientation was observed accompanying the cell migration. Judging from the different temporal responses of the orientation and the migration, it is proposed that the two EF-induced responses may involve different signaling pathways.
authors.affiliation: Institute of Biomedical Engineering, National Taiwan University, Taiwan.
authors.forename: Ching-Wen
authors.initials: CW
authors.lastname: Huang
authors.forename: Ji-Yen
authors.initials: JY
authors.lastname: Cheng
authors.forename: Meng-Hua
authors.initials: MH
authors.lastname: Yen
authors.forename: Tai-Horng
authors.initials: TH
authors.lastname: Young
mesh_terms: D018929:Cell Culture Techniques / Q000295:instrumentation*; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000528:radiation effects*; D002469:Cell Separation / Q000295:instrumentation*; D004563:Electrochemistry / Q000295:instrumentation*; D006801:Humans; D008175:Lung Neoplasms / Q000503:physiopathology*; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D008846:Micromanipulation / Q000295:instrumentation*; D011829:Radiation Dosage