Evaluation of new selective culture media and a rapid fluorescence in situ hybridization assay for identification of Clostridium difficile from stool samples.
Two new Clostridium difficile-selective agars, from Oxoid (according to Brazier) and from BD, were compared with cycloserine-cefoxitin-fructose agar (Oxoid) for their sensitivity of recovery of toxigenic C. difficile from stool samples. For the culture-positive samples, the sensitivities were 84.0, 42.6 and 90.4 %, respectively. In addition, a C. difficile-specific fluorescence in situ hybridization assay was developed, facilitating rapid and reliable identification of cultured isolates.
Bloedt
Kathrin
K
Institute of Medical Microbiology and Hygiene, University Hospital of Ulm, Ulm, Germany.
Riecker
Melanie
M
Institute of Medical Microbiology and Hygiene, University Hospital of Ulm, Ulm, Germany.
Poppert
Sven
S
Department of Molecular Parasitology, Bernhard Nocht Institute for Tropical Medicine, Hamburg, Germany.
Wellinghausen
Nele
N
Dr Gaertner & Partner Laboratories, Ravensburg, Germany.|Institute of Medical Microbiology and Hygiene, University Hospital of Ulm, Ulm, Germany.
D000328:Adult; D002648:Child; D016360:Clostridioides difficile / Q000302:isolation & purification*; D003470:Culture Media / Q000737:chemistry*; D004761:Enterocolitis, Pseudomembranous / Q000175:diagnosis* / Q000382:microbiology*; D005243:Feces / Q000382:microbiology*; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*
D003470:Culture Media