title: Lipin is required for efficient breakdown of the nuclear envelope in Caenorhabditis elegans.
abstract: The nuclear envelope (NE) is a double lipid bilayer that separates nucleus and cytoplasm. In metazoa, NE breakdown (NEBD) occurs during prophase and NE reformation around segregated chromatids occurs at anaphase-telophase. We identified Caenorhabditis elegans Lipin homologue (called Lpin-1) as an essential factor with roles in endoplasmic reticulum (ER) organization and NEBD. RNAi-mediated downregulation of Lpin-1 had no effect on timely entry into mitosis or on the early steps of NEBD, but Lpin-1 was required for disassembly of the nuclear lamina during late NEBD. This Lpin-1 requirement appears to be separable from the effect of Lpin-1 on the peripheral ER.
authors.affiliation: European Molecular Biology Laboratory, Meyerhofstrasse 1, 69117, Heidelberg, Germany.
authors.forename: Mátyás
authors.initials: M
authors.lastname: Gorjánácz
authors.forename: Iain W
authors.initials: IW
authors.lastname: Mattaj
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D017173:Caenorhabditis elegans / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D029742:Caenorhabditis elegans Proteins / Q000502:physiology; D048750:Cell Nucleus Division / Q000502:physiology*; D004721:Endoplasmic Reticulum / Q000502:physiology; D008938:Mitosis / Q000502:physiology; D009685:Nuclear Envelope / Q000378:metabolism / Q000502:physiology*; D034881:Nuclear Lamina / Q000378:metabolism / Q000502:physiology; D009687:Nuclear Proteins / Q000502:physiology
chemical_list: D029742:Caenorhabditis elegans Proteins; D009687:Nuclear Proteins