title: Multiplex, fluorescent single-strand conformation polymorphism using stepped polymerase chain reaction primers.
abstract: A method for high-throughput screening for a well-characterized mutation at the APC locus was developed using stepped PCR primers, pooled product, and fluorescent single-strand conformation polymorphism (SSCP). Using a panel of two known normals, three known heterozygous mutants, and one known homozygous mutant, eight stepped PCR products were developed that showed the same mobility shift on a PE Biosystems 373A. Using this method, up to eight different samples can be pooled for same lane analysis.
authors.affiliation: Genomics Core Facility, University of Utah, Salt Lake City, UT 84132, USA.
authors.forename: R
authors.initials: R
authors.lastname: Scholl
authors.forename: A
authors.initials: A
authors.lastname: Walker
authors.forename: L W
authors.initials: LW
authors.lastname: Ballard