Comparative genomic hybridization on spotted oligonucleotide microarrays.
Recent advances in DNA microarray technology have enabled researchers to comprehensively characterize the complex genomes of higher eukaryotic organisms at an unprecedented level of detail. Array-based comparative genomic hybridization (Array-CGH) has been widely used for detecting DNA copy number alterations on a genomic scale, where the mapping resolution is limited only by the number of probes on the DNA microarray. In this chapter, we present a validated protocol utilizing print-tip spotted HEEBO (Human Exonic Evidence Based Oligonucleotide) microarrays for conducting array-CGH using as little as 25 ng of genomic DNA from a wide variety of sources, including cultured cell lines and clinical specimens, with high spatial resolution and array-to-array reproducibility.
Kim
Young H
YH
Department of Pathology, Stanford University, Stanford, CA, USA.
Pollack
Jonathan R
JR
D055028:Comparative Genomic Hybridization / Q000295:instrumentation / Q000379:methods; D016678:Genome; D006801:Humans; D007091:Image Processing, Computer-Assisted / Q000379:methods; D020411:Oligonucleotide Array Sequence Analysis / Q000295:instrumentation / Q000379:methods; D015203:Reproducibility of Results