Quantum dot molecular beacons for DNA detection.
Molecular beacons have become an important fluorescent probe for sequence-specific DNA detection. To improve the sensitivity and robustness of molecular beacon assays, fluorescent semiconductor quantum dots (QDs) are now being used as the fluorescent moiety for molecular beacon synthesis. Multiple linkage strategies can be used for attaching molecular beacon DNA to QDs, and multiple quenchers, including gold particles, can be used for fluorescence quenching. Covalent attachment of QDs to DNA can be achieved through amide linkage, and affinity-based attachment can be achieved with streptavidin-biotin linkage. We have shown that these linkage strategies can be used to successfully create quantum dot molecular beacons that can be used in DNA detection assays with high specificity.
Cady
Nathaniel C
NC
College of Nanoscale Science and Engineering, University at Albany, Albany, NY 12203, USA. ncady@uamail.albany.edu
D000577:Amides / Q000737:chemistry; D015374:Biosensing Techniques; D001710:Biotin; D004247:DNA / Q000032:analysis*; D024202:Electrophoretic Mobility Shift Assay; D005456:Fluorescent Dyes / Q000737:chemistry; D005470:Fluorometry; D006046:Gold; D053768:Metal Nanoparticles; D009841:Oligonucleotides / Q000737:chemistry; D045663:Quantum Dots; D019809:Streptavidin
D000577:Amides; D005456:Fluorescent Dyes; D009841:Oligonucleotides; D001710:Biotin; D006046:Gold; D004247:DNA; D019809:Streptavidin