title: Stable expression of the chlorocatechol dioxygenase gene from Ralstonia eutropha NH9 in hybrid poplar cells.
abstract: The cbnA gene encoding chlorocatechol dioxygenase from the soil bacterium Ralstonia eutropha NH9 under the control of a modified cauliflower mosaic virus 35S promoter was introduced into a hybrid poplar (Populus tremula x P. tremuloides). Integration of the cbnA gene in transgenic poplar was confirmed by PCR and genomic Southern blot analysis. Expression of the cbnA gene was analyzed by Western blot analysis. Transgenic poplar calli efficiently converted 3-chlorocatechol to 2-chloro-cis,cis-muconate.
authors.affiliation: Forest Tree Breeding Center, Hitachi, Ibaraki, Japan.
authors.forename: Yasunori
authors.initials: Y
authors.lastname: Ohmiya
authors.forename: Toshiro
authors.initials: T
authors.lastname: Ono
authors.forename: Toru
authors.initials: T
authors.lastname: Taniguchi
authors.forename: Naoei
authors.initials: N
authors.lastname: Itahana
authors.forename: Naoto
authors.initials: N
authors.lastname: Ogawa
authors.forename: Kiyotaka
authors.initials: K
authors.lastname: Miyashita
authors.forename: Kunio
authors.initials: K
authors.lastname: Ohmiya
authors.forename: Kazuo
authors.initials: K
authors.lastname: Sakka
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Kimura
mesh_terms: D015139:Blotting, Southern; D015153:Blotting, Western; D002853:Chromatography, Liquid; D020118:Cupriavidus necator / Q000201:enzymology*; D049308:Dioxygenases / Q000235:genetics*; D017343:Genes, Plant; D030821:Plants, Genetically Modified; D016133:Polymerase Chain Reaction; D032107:Populus / Q000166:cytology / Q000235:genetics*
chemical_list: D049308:Dioxygenases; C044173:chlorocatechol 1,2-dioxygenase