Detection of retinal metabolic stress resulting from central serous retinopathy.
OBJECTIVE
To test whether eyes with central serous retinopathy have elevated retinal flavoprotein fluorescence (FPF) using a novel clinical imaging method.


METHODS
Three male patients with unilateral central serous retinopathy were examined for FPF at 535 nm induced by 1-msec flashes of 467 nm light. FPF was captured with an electron multiplying charged-coupled device camera with a 512 x 512 pixel chip. Average intensity of retinal FPF for each affected eye was compared with the contralateral, unaffected eye and with six age-matched control eyes by analyzing histograms of pixel intensities plotted for each eye.


RESULTS
For each patient, the central serous retinopathy-affected eye had a significantly greater retinal FPF when compared with the retinal FPF of the unaffected eye. Eyes affected with central serous retinopathy had retinal FPF values that averaged 98% greater than the retinal FPF of age-matched control eyes.


CONCLUSIONS
Flavoprotein fluorescence analysis may be useful for rapidly and noninvasively identifying metabolic tissue stress of central serous retinopathy.
Field
Matthew G
MG
Department of Ophthalmology and Visual Sciences, University of Michigan, Ann Arbor, Michigan 48105, USA.
Elner
Victor M
VM
Park
Seung
S
Hackel
Richard
R
Heckenlively
John R
JR
Elner
Susan G
SG
Petty
Howard R
HR
D000328:Adult; D015415:Biomarkers; D056833:Central Serous Chorioretinopathy / Q000378:metabolism* / Q000473:pathology / Q000503:physiopathology; D005420:Flavoproteins / Q000032:analysis / Q000378:metabolism; D005451:Fluorescein Angiography; D005453:Fluorescence; D006801:Humans; D008297:Male; D018384:Oxidative Stress; D015203:Reproducibility of Results; D012160:Retina / Q000378:metabolism* / Q000473:pathology / Q000503:physiopathology
D015415:Biomarkers; D005420:Flavoproteins