Simultaneous determination of thiamine and its phosphate esters by a liquid chromatographic method based on post-column photolysis and chemiluminescence detection.
A sensitive method for the post-column detection of thiamine (T) and its phosphate esters is described. The procedure is based on the on-line photolysis of the analytes into photoproducts which have a strong enhancing effect on the CL permanganate-luminol reaction. The complete separation of the thiamines was obtained on a RP-amide C(16) column in isocratic elution with an analysis time of less than 7 min. Under the optimum conditions, analytical curves, based on standard solutions, were linear over the range 10-1000 nM for thiamine and 100-2000 nM for its mono- and di-phosphate esters. Intra- and inter-day precision values of less than 1.14% relative standard deviation (RSD) (n=10) and 1.86% RSD (n=15), respectively, were obtained. The method was successfully applied to the determination of the thiamines in pharmaceutical preparations and baby foods.
Pérez-Ruiz
Tomás
T
Department of Analytical Chemistry, Faculty of Chemistry, University of Murcia, 30071 Murcia, Spain. tpr@um.es
Martínez-Lozano
Carmen
C
García-Martínez
María Dolores
MD
D002853:Chromatography, Liquid / Q000379:methods*; D007225:Infant Food; D015203:Reproducibility of Results; D013831:Thiamine / Q000032:analysis*; D013833:Thiamine Monophosphate / Q000032:analysis*; D013835:Thiamine Pyrophosphate / Q000032:analysis*; D014803:Vitamin B Complex / Q000032:analysis
D014803:Vitamin B Complex; D013833:Thiamine Monophosphate; D013835:Thiamine Pyrophosphate; D013831:Thiamine