HLA-B*15 subtypes in Burmese population by sequence-based typing.
Human leukocyte antigen (HLA)-B*15 encompasses an increasing number of subtypes of more than 150. Frequency studies and a strong genetic association between HLA subtypes and susceptibility to drug hypersensitivity have been reported in different ethnic populations. To identify HLA-B*15 subtypes in Burmese using sequence-based typing (SBT) method, we selected 65 HLA-B*15-positive samples from 170 unrelated healthy Burmese who were genotyped HLA-B* by polymerase chain reaction with the sequence-specific primer method. The frequency of HLA-B*15 in Burmese was found to be 38.2%. By the SBT method, results showed 10 alleles of HLA-B*15 subtypes. Four common alleles, B*1502 (45.2%), B*1532 (16.4%), B*1525 (12.3%), and B*1501 (8.2%), were found in 82.1% of HLA-B*15-positive Burmese. Whereas the B*1501 was the highest in the Caucasians, Koreans, and Japanese, the highest frequency of HLA-B*15 alleles in Burmese was B*1502 (45.2%) that is similar to the frequency found in northeastern Thais and Vietnamese. This study is the first report of HLA-B*15 subtypes in Burmese. These results will provide the basic data in the further study in transplantations, genetic association with diseases, and drug hypersensitivity.
Kongmaroeng
C
C
PhD Program in Biomedical Science, Faculty of Graduate School, Khon Kaen University, Khon Kaen, Thailand. arunrant@kku.ac.th
Romphruk
A
A
Ruangwerayut
R
R
Paupairoj
C
C
Leelayuwat
C
C
Inoko
H
H
Romphruk
A
A
D005787:Gene Frequency; D005838:Genotype; D015235:HLA-B Antigens / Q000032:analysis / Q000145:classification / Q000235:genetics*; D059910:HLA-B15 Antigen; D006650:Histocompatibility Testing / Q000379:methods*; D006801:Humans; D002052:Myanmar / Q000208:ethnology; D011110:Polymorphism, Genetic; D017422:Sequence Analysis, DNA; D013785:Thailand
D015235:HLA-B Antigens; D059910:HLA-B15 Antigen