title: Spectroscopic diagnosis of laryngeal carcinoma using near-infrared Raman spectroscopy and random recursive partitioning ensemble techniques.
abstract: In this work, we evaluated the diagnostic ability of near-infrared (NIR) Raman spectroscopy associated with the ensemble recursive partitioning algorithm based on random forests for identifying cancer from normal tissue in the larynx. A rapid-acquisition NIR Raman system was utilized for tissue Raman measurements at 785 nm excitation, and 50 human laryngeal tissue specimens (20 normal; 30 malignant tumors) were used for NIR Raman studies. The random forests method was introduced to develop effective diagnostic algorithms for classification of Raman spectra of different laryngeal tissues. High-quality Raman spectra in the range of 800-1800 cm(-1) can be acquired from laryngeal tissue within 5 seconds. Raman spectra differed significantly between normal and malignant laryngeal tissues. Classification results obtained from the random forests algorithm on tissue Raman spectra yielded a diagnostic sensitivity of 88.0% and specificity of 91.4% for laryngeal malignancy identification. The random forests technique also provided variables importance that facilitates correlation of significant Raman spectral features with cancer transformation. This study shows that NIR Raman spectroscopy in conjunction with random forests algorithm has a great potential for the rapid diagnosis and detection of malignant tumors in the larynx.
authors.affiliation: Bioimaging Laboratory, Department of Bioengineering, Faculty of Engineering, National University of Singapore, 9 Engineering Drive 1, Singapore 117576.
authors.forename: Seng Khoon
authors.initials: SK
authors.lastname: Teh
authors.forename: Wei
authors.initials: W
authors.lastname: Zheng
authors.forename: David P
authors.initials: DP
authors.lastname: Lau
authors.forename: Zhiwei
authors.initials: Z
authors.lastname: Huang
mesh_terms: D000465:Algorithms; D005240:Feasibility Studies; D006801:Humans; D007259:Infrared Rays; D007822:Laryngeal Neoplasms / Q000175:diagnosis* / Q000473:pathology; D007830:Larynx / Q000166:cytology / Q000473:pathology; D011336:Probability; D013059:Spectrum Analysis, Raman / Q000379:methods*