title: HIV endocytosis after dendritic cell to T cell viral transfer leads to productive virus infection.
abstract: Contacts between HIV-producing T cells and primary CD4+ T cells may induce the uptake of HIV by target cells that are endocytosed into trypsin-resistant compartments. We have now compared the mechanism of virus transmission from T cell-to-T cell versus infected dendritic cells (DCs)-to-T cell. In cocultures of HIV-1-infected DCs with primary CD4+ T cells, virus transmission to the target cells was resistant to trypsin treatment and could only be prevented by the anti-SUgp120 antibody IgGb12 but not by TAK-779, C34 or AZT. Importantly, upon stimulation of purified HIV-1-loaded CD4+ T cells with PHA/IL-2, cells became productively infected as measured by intracellular CAp24 staining and antigen determination in the cell supernatant. These results suggest that the viral endocytic transfer may represent a escape mechanism in the presence of drugs targeting HIV-1 entry or the host immune system.
authors.affiliation: Retrovirology Laboratory IrsiCaixa, Hospital Universitari Germans Trias i Pujol, Universitat Autònoma de Barcelona, Badalona, Spain.
authors.forename: Imma
authors.initials: I
authors.lastname: Clotet-Codina
authors.forename: Berta
authors.initials: B
authors.lastname: Bosch
authors.forename: Jordi
authors.initials: J
authors.lastname: Senserrich
authors.forename: María Teresa
authors.initials: MT
authors.lastname: Fernández-Figueras
authors.forename: Ruth
authors.initials: R
authors.lastname: Peña
authors.forename: Ester
authors.initials: E
authors.lastname: Ballana
authors.forename: Margarita
authors.initials: M
authors.lastname: Bofill
authors.forename: Bonaventura
authors.initials: B
authors.lastname: Clotet
authors.forename: José A
authors.initials: JA
authors.lastname: Esté
mesh_terms: D015496:CD4-Positive T-Lymphocytes / Q000821:virology*; D002478:Cells, Cultured; D018920:Coculture Techniques; D003713:Dendritic Cells / Q000821:virology*; D004705:Endocytosis; D015497:HIV-1 / Q000254:growth & development*; D006801:Humans