title: Planomicrobium glaciei sp. nov., a psychrotolerant bacterium isolated from a glacier.
abstract: A novel aerobic, psychrotolerant, yellow-to-orange bacterium (strain 0423(T)) was isolated from the China No. 1 glacier. Strain 0423(T) displayed phenotypic and chemotaxonomic features of the genus Planomicrobium, containing anteiso-C(15 : 0) and iso-C(14 : 0) as the major fatty acids. The temperature range for growth was 4-28 degrees C, with optimum growth at 20-21 degrees C. The genomic DNA G+C content was 49 mol%. Phylogenetic analysis based on 16S rRNA gene sequence similarity showed that strain 0423(T) was related to members of the genus Planomicrobium, sharing the highest sequence similarities with the type strains of Planomicrobium chinense, P. mcmeekinii, P. okeanokoites and P. koreense. On the basis of phenotypic characteristics, phylogenetic analysis and DNA-DNA relatedness data, a novel species, Planomicrobium glaciei sp. nov., is proposed. The type strain is 0423(T) (=CGMCC 1.6846(T) =JCM 15088(T)).
authors.affiliation: China General Microbiological Culture Collection Center and State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PR China.
authors.forename: De-Chao
authors.initials: DC
authors.lastname: Zhang
authors.forename: Hong-Can
authors.initials: HC
authors.lastname: Liu
authors.forename: Yu-Hua
authors.initials: YH
authors.lastname: Xin
authors.forename: Yong
authors.initials: Y
authors.lastname: Yu
authors.forename: Pei-Jin
authors.initials: PJ
authors.lastname: Zhou
authors.forename: Yu-Guang
authors.initials: YG
authors.lastname: Zhou
mesh_terms: D015373:Bacterial Typing Techniques; D002681:China; D003080:Cold Temperature; D004269:DNA, Bacterial / Q000032:analysis; D004275:DNA, Ribosomal / Q000032:analysis; D005227:Fatty Acids / Q000032:analysis; D006094:Gram-Positive Bacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology; D046448:Ice Cover / Q000382:microbiology*; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010641:Phenotype; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013045:Species Specificity
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S