title: Expressions of adiponectin receptors in placenta and their correlation with preeclampsia.
abstract: BACKGROUND
  Adiponectin and its receptors, adiponectin receptor 1 (Adipo-R1) and adiponectin receptor 2 (Adipo-R2), may contribute to preeclampsia; however, the reports up to date are conflicting. Here we further explore this issue.
  
  
  METHODS
  We studied 20 pregnant women with term normal pregnancy, 22 women with severe preeclampsia, and 12 mild cases. The levels of Adipo-Rs' protein and messenger RNA (mRNA) were detected by immunohistochemistry and real-time quantitative polymerized chain reaction (PCR) analysis, respectively.
  
  
  RESULTS
  The expression of Adipo-R2, but not Adipo-R1, was observed in the cytoplasm of both placental cytotrophoblasts and syncytiotrophoblasts of the mild preeclempsia, severe cases and normal pregnancy group. There was no significant difference of Adipo-R2 protein level between the maternal side and the fetal side in each group (all P > .05). Adipo-R2 protein and mRNA levels in severe preeclamptic group were significantly higher than those of mild cases (P < .001) and normal pregnancy group (P < .001). There was also no significant difference of Adipo-R2 protein and mRNA levels between term delivery and preterm delivery in severe preeclamptic group (P > .05). Nonetheless, both the protein and mRNA levels were significantly higher in comparison to those of the normal group (P < .05).
  
  
  CONCLUSIONS
  The abnormality of Adipo-R2 may be associated with the pathogenesis of preeclampsia.
authors.affiliation: Department of Obstetrics and Gynecology, West China Second University Hospital, Sichuan University, Chengdu, China.
authors.lastname: Tie Weiwei
authors.lastname: Yu Haiyan
authors.lastname: Chen Juan
authors.lastname: Wang Xiaodong
authors.lastname: Chen Weibo
authors.lastname: Zhou Rong
mesh_terms: D000328:Adult; D003430:Cross-Sectional Studies; D003593:Cytoplasm / Q000737:chemistry / Q000378:metabolism; D005260:Female; D005333:Fetus / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental / Q000502:physiology*; D006801:Humans; D010920:Placenta / Q000737:chemistry / Q000378:metabolism*; D011225:Pre-Eclampsia / Q000175:diagnosis* / Q000378:metabolism*; D011247:Pregnancy; D054419:Receptors, Adiponectin / Q000096:biosynthesis*; D012720:Severity of Illness Index; D014327:Trophoblasts / Q000737:chemistry / Q000378:metabolism
chemical_list: C475418:ADIPOR1 protein, human; C475420:ADIPOR2 protein, human; D054419:Receptors, Adiponectin