Analysis of major isoforms of human growth hormone before and after intensive physical exercise.
We developed a semiquantitative method for the analysis of main growth hormone isoforms. The use of immunoaffinity sorbents, two-dimensional electrophoresis, and immunoblotting allows detection of more than 90% circulating growth hormone. It was demonstrated that the proportion of growth hormone isoforms in human serum before and after strenuous exercise remained unchanged.
Sakharov
D A
DA
Russian Physical Training and Sports Institute, Moscow. dimitri_sakharov@mail.ru
Thevis
M
M
Tonevitsky
A G
AG
D000293:Adolescent; D015180:Electrophoresis, Gel, Two-Dimensional; D015444:Exercise / Q000502:physiology*; D005260:Female; D019382:Human Growth Hormone / Q000032:analysis / Q000097:blood*; D006801:Humans; D015151:Immunoblotting; D008297:Male; D020033:Protein Isoforms / Q000032:analysis / Q000097:blood*; D055815:Young Adult
D020033:Protein Isoforms; D019382:Human Growth Hormone