title: Directed formation of lipid membrane microdomains as high affinity sites for His-tagged proteins.
abstract: Lipid membranes composed of an iminodiacetic acid functionalized lipid, DSIDA, in a POPC matrix exhibited switchable properties via Cu(2+) recognition to rapidly assemble microdomains that act as high affinity sites for His-tagged proteins. The microdomains demonstrated an order of magnitude enhanced affinity for the proteins compared to homogeneously functionalized POPC membranes with Ni(2+)-NTA DOGS or Cu(2+)-DOIDA, while a rapid release and restoration of the original membrane was accomplished with micromolar concentrations of EDTA.
authors.affiliation: Sandia National Laboratories, P.O. Box 969, Livermore, California 94551, USA.
authors.forename: Carl C
authors.initials: CC
authors.lastname: Hayden
authors.forename: Jane S
authors.initials: JS
authors.lastname: Hwang
authors.forename: Elisa A
authors.initials: EA
authors.lastname: Abate
authors.forename: Michael S
authors.initials: MS
authors.lastname: Kent
authors.forename: Darryl Y
authors.initials: DY
authors.lastname: Sasaki
mesh_terms: D000818:Animals; D002352:Carrier Proteins / Q000737:chemistry / Q000378:metabolism; D003300:Copper / Q000378:metabolism*; D006639:Histidine / Q000737:chemistry; D007098:Imino Acids / Q000737:chemistry* / Q000378:metabolism; D050356:Lipid Metabolism; D008055:Lipids / Q000737:chemistry*; D058619:Maltose-Binding Proteins; D021962:Membrane Microdomains / Q000737:chemistry* / Q000378:metabolism*; D008567:Membranes, Artificial; D010713:Phosphatidylcholines / Q000737:chemistry; D011506:Proteins / Q000737:chemistry / Q000378:metabolism*
chemical_list: D002352:Carrier Proteins; D007098:Imino Acids; D008055:Lipids; D058619:Maltose-Binding Proteins; D008567:Membranes, Artificial; D010713:Phosphatidylcholines; D011506:Proteins; D006639:Histidine; D003300:Copper; C028694:1-palmitoyl-2-oleoylphosphatidylcholine; C008109:iminodiacetic acid