Protein microarray analysis of nasal polyps from aspirin-sensitive and aspirin-tolerant patients with chronic rhinosinusitis.
BACKGROUND
The purpose of this study was to apply protein microarray technology to the study of sinonasal tissue and to identify differential protein expression in nasal polyps from aspirin-sensitive (AS) versus aspirin-tolerant (AT) patients with chronic rhinosinusitis (CRS) and CRS with nasal polyps (CRSwNPs).


METHODS
Nasal polyp specimens were prospectively obtained from two groups of patients with CRSwNP. The test group (AS) consisted of five patients that were diagnosed with CRSwNP and intolerance to aspirin based on medical history and physical exam. The control group (AT) consisted of four AT patients with CRSwNP. Protein was extracted and labeled from harvested polyps and the Sigma Panorama Antibody Microarray-Cell Signaling Kit was used to identify differences in protein expression between the two polyp groups. Western blot analysis was used to validate the results of the protein microarray.


RESULTS
The protein microarray showed a greater than twofold change in expression of both beta-adaptin and heat shock protein 70 (HSP70). Western blot analysis confirmed up-regulation of beta-adaptin and HSP70 in nasal polyp tissue from AS patients.


CONCLUSIONS
Pooled samples of AS and AT nasal polyps evaluated by protein microarray show distinct protein expression profiles in the stress response and receptor-mediated endocytosis pathways. This study establishes the successful application of protein microarray technology to study nasal polyposis, which in turn can be validated by Western blot analysis.
Zander
Kelly A
KA
Department of Otolaryngology-Head and Neck Surgery, University of Colorado Denver, Denver, Colorado, USA.
Saavedra
Milene T
MT
West
James
J
Scapa
Victor
V
Sanders
Linda
L
Kingdom
Todd T
TT
D033966:Adaptor Protein Complex beta Subunits / Q000032:analysis / Q000235:genetics; D000328:Adult; D000368:Aged; D001241:Aspirin / Q000494:pharmacology*; D053181:Caspase 8 / Q000032:analysis; D053453:Caspase 9 / Q000032:analysis; D002908:Chronic Disease; D004361:Drug Tolerance; D005260:Female; D018840:HSP70 Heat-Shock Proteins / Q000032:analysis / Q000235:genetics; D006801:Humans; D008297:Male; D008875:Middle Aged; D009298:Nasal Polyps / Q000378:metabolism*; D040081:Protein Array Analysis / Q000379:methods*; D012220:Rhinitis / Q000378:metabolism*; D012852:Sinusitis / Q000378:metabolism*
D033966:Adaptor Protein Complex beta Subunits; D018840:HSP70 Heat-Shock Proteins; D053181:Caspase 8; D053453:Caspase 9; D001241:Aspirin