title: Identification of genes expressed during the self-incompatibility response in perennial ryegrass (Lolium perenne L.).
abstract: Self-incompatibility (SI) in Lolium perenne is controlled gametophytically by the S-Z two-locus system. S and Z loci mapped to L. perenne linkage groups 1 and 2, respectively, with their corresponding putative-syntenic regions on rice chromosome 5 (R5) and R4. None of the gene products of S and Z have yet been identified. SI cDNA libraries were developed to enrich for SI expressed genes in L. perenne. Transcripts were identified from the SI libraries that were orthologous to sequences on rice R4 and R5. These represent potential SI candidate genes. Altogether ten expressed SI candidate genes were identified. A rapid increase in gene expression within two minutes after pollen-stigma contact was revealed, reaching a maximum between 2 and 10 min. The potential involvement of these genes in the SI reactions is discussed.
authors.affiliation: Teagasc Crops Research Centre, Oak Park, Carlow, Ireland.
authors.forename: Bicheng
authors.initials: B
authors.lastname: Yang
authors.forename: Daniel
authors.initials: D
authors.lastname: Thorogood
authors.forename: Ian P
authors.initials: IP
authors.lastname: Armstead
authors.forename: F C H
authors.initials: FC
authors.lastname: Franklin
authors.forename: Susanne
authors.initials: S
authors.lastname: Barth
mesh_terms: D002874:Chromosome Mapping; D018076:DNA, Complementary / Q000235:genetics; D018744:DNA, Plant / Q000235:genetics; D015870:Gene Expression; D015723:Gene Library; D017343:Genes, Plant; D008129:Lolium / Q000235:genetics* / Q000502:physiology*; D008969:Molecular Sequence Data; D054817:Pollination / Q000235:genetics / Q000502:physiology; D012098:Reproduction / Q000235:genetics / Q000502:physiology; D016324:Sequence Tagged Sites
chemical_list: D018076:DNA, Complementary; D018744:DNA, Plant