Effect of extracts from pine needle against oxidative DNA damage and apoptosis induced by hydroxyl radical via antioxidant activity.
In this study, the protective effects of water extracts from pine needle (WEPN) against DNA damage and apoptosis induced by hydroxyl radical were investigated in non-cellular and cellular system. WEPN exhibited strong scavenging action on hydroxyl radical and intracellular ROS, and chelating action of Fe(2+) ion. WEPN inhibited oxidative DNA damage by hydroxyl radical. Also, WEPN prevented the cells from oxidative damage through lowering p21 and BAX protein expression, blocking the cleavage of PARP and increasing Bcl-2 protein, which was confirmed by Hoechst 33342 staining. These data indicate that WEPN possesses a spectrum of antioxidant and DNA-protective properties common to cancer chemopreventive agents.
Jeong
Jin Boo
JB
College of Natural Science, Andong National University, 388, Songcheon-Dong, Andong 760749, Republic of Korea.
Seo
Eul Won
EW
Jeong
Hyung Jin
HJ
D016475:3T3 Cells; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D010584:Bacteriophage phi X 174 / Q000235:genetics; D001562:Benzimidazoles; D015153:Blotting, Western; D004249:DNA Damage; D004996:Ethidium; D005456:Fluorescent Dyes; D017665:Hydroxyl Radical / Q000037:antagonists & inhibitors* / Q000633:toxicity*; D007150:Immunohistochemistry; D007502:Iron Chelating Agents / Q000737:chemistry / Q000494:pharmacology; D051379:Mice; D016877:Oxidants / Q000633:toxicity*; D018384:Oxidative Stress / Q000187:drug effects*; D028223:Pinus / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology; D018515:Plant Leaves / Q000737:chemistry; D017382:Reactive Oxygen Species / Q000378:metabolism
D000975:Antioxidants; D001562:Benzimidazoles; D005456:Fluorescent Dyes; D007502:Iron Chelating Agents; D016877:Oxidants; D010936:Plant Extracts; D017382:Reactive Oxygen Species; D017665:Hydroxyl Radical; D004996:Ethidium; C017807:bisbenzimide ethoxide trihydrochloride