Demonstration of Borrelia burgdorferi sensu stricto infection in ticks from the northeast of Mexico.
Borrelia burgdorferisensu lato infection has been confirmed in clinical cases in the northeast of Mexico; however, the bacterium has not been identified as infecting the tick vector Ixodes, Amblyomma and Dermacentor ticks were collected from mammals and plants in northeastern Mexico and examined for Borrelia. Eighteen of 214 ticks were PCR-positive for the fla and 16S rRNA genes and 15 for the ospA gene. Southern blotting with a fla probe and sequencing of ospA genes confirmed infection with B. burgdorferi sensu stricto. These findings, together with reports of indigenous cases, fulfil the criteria that allow northeastern Mexico to be considered as a zone endemic for Lyme disease.
Gordillo-Pérez
G
G
Unidad de Investigación Médica en Enfermedades Infecciosas, Hospital de Pediatría, Centro Medico Nacional SXXI-IMSS, Mexico City, Mexico. lugope2@yahoo.com.mx
Vargas
M
M
Solórzano-Santos
F
F
Rivera
A
A
Polaco
O J
OJ
Alvarado
L
L
Muñóz
O
O
Torres
J
J
D000818:Animals; D000954:Antigens, Surface / Q000235:genetics; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics; D001426:Bacterial Proteins / Q000235:genetics; D001428:Bacterial Vaccines / Q000235:genetics; D015139:Blotting, Southern; D001899:Borrelia Infections / Q000382:microbiology / Q000662:veterinary*; D025065:Borrelia burgdorferi / Q000235:genetics / Q000302:isolation & purification*; D004269:DNA, Bacterial / Q000235:genetics; D026863:Ixodidae / Q000382:microbiology*; D008074:Lipoproteins / Q000235:genetics; D008800:Mexico; D016133:Polymerase Chain Reaction / Q000379:methods; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA
D000954:Antigens, Surface; D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D001428:Bacterial Vaccines; D004269:DNA, Bacterial; D008074:Lipoproteins; C072045:OspA protein; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S