Parallax: high accuracy three-dimensional single molecule tracking using split images.
Three-dimensional (3D) tracking can provide valuable biological insights that are missing in conventional microscopy. Here we developed a single molecule 3D tracking microscopy technique, named Parallax, with high localization precision and temporal resolution. We demonstrated its capabilities by studying the 3D trafficking of glucose-transporter-4 containing vesicles in living adipocytes as well as the walking path of single myosin VI molecules along actin filaments.
Sun
Yujie
Y
Pennsylvania Muscle Institute, University of Pennsylvania, 3700 Hamilton Walk, Philadelphia, Pennsylvania 19104, USA.
McKenna
Jennine Dawicki
JD
Murray
John M
JM
Ostap
E Michael
EM
Goldman
Yale E
YE
D017667:Adipocytes / Q000737:chemistry; D000818:Animals; D051275:Glucose Transporter Type 4 / Q000737:chemistry; D007090:Image Interpretation, Computer-Assisted / Q000379:methods; D021621:Imaging, Three-Dimensional / Q000379:methods*
D051275:Glucose Transporter Type 4