title: Immunohistochemical discrimination of plasmacytoid dendritic cells from myeloid dendritic cells in human pathological tissues.
abstract: Until now, no method has been available to discriminate mature plasmacytoid DC (pDC) from myeloid DC (mDC) immunohistochemically. In this study, we report that these DC-subsets can be distinguished in routine pathological sections. Immature and mature monocyte-derived DCs (MoDCs) were S100 calcium binding protein B (S100B)+, while pDCs generated from pDC-precursors were S100B-. In contrast, both mature MoDC and pDC were fascin+. Epidermal Langerhans cells (LCs) were S100B+/fascin-. Although the majority of DCs were S100B+/fascin+ in the dermis with nonspecific inflammation, dermal DCs were mostly S100B-/fascin+ in psoriasis vulgaris, in which type I interferon secreted by pDC-precursors is thought to play a major role. S100B+/fascin+ DCs were accumulated in the superficial lymph node (LN), while they were scarce in the deep LN. In the superficial LN with dermatopathic lymphadenitis, a large number of S100B+/fascin+ DCs were accumulated in the T-zones, where numerous LC-derived DCs are accumulated. In contrast, almost all DCs were S100B-/fascin+ in the superficial LN with Kikuchi's lymphadenitis, in which numerous pDC-precursors are known to be present. In contrast to the superficial LN, the deep LN contained numerous S100B-/fascin+ DCs and a few S100B+ DCs. Thus, the distributions of S100B+ DC or S100B-/fascin+ DC correspond to the putative distribution of mDC or mature pDC, respectively.
authors.affiliation: Graduate School of Health Sciences, Okayama University.
authors.forename: Yuriko
authors.initials: Y
authors.lastname: Nishikawa
authors.forename: Hiaki
authors.initials: H
authors.lastname: Sato
authors.forename: Takashi
authors.initials: T
authors.lastname: Oka
authors.forename: Tadashi
authors.initials: T
authors.lastname: Yoshino
authors.forename: Kiyoshi
authors.initials: K
authors.lastname: Takahashi
mesh_terms: D002352:Carrier Proteins / Q000032:analysis*; D019070:Cell Lineage; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000145:classification / Q000166:cytology* / Q000473:pathology; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods*; D007801:Langerhans Cells / Q000166:cytology; D008199:Lymphadenitis / Q000473:pathology; D008840:Microfilament Proteins / Q000032:analysis*; D009414:Nerve Growth Factors / Q000032:analysis*; D011565:Psoriasis / Q000473:pathology; D064568:S100 Calcium Binding Protein beta Subunit; D009418:S100 Proteins / Q000032:analysis*; D012871:Skin Diseases / Q000473:pathology*; D014018:Tissue Distribution
chemical_list: D002352:Carrier Proteins; D008840:Microfilament Proteins; D009414:Nerve Growth Factors; D064568:S100 Calcium Binding Protein beta Subunit; D009418:S100 Proteins; C579160:S100B protein, human; C020324:fascin