title: Separation and quantitation of Z-isomer in lanoconazole by normal phase HPLC.
abstract: An isocratic normal phase high-performance liquid chromatographic (NP-HPLC) method has been developed and validated for the quantitation of Z-isomer in lanoconazole. Separation was achieved with a Thermo Hypersil Silica column. The ratio of 2-propanol, n-hexane and triethylamine in the mobile phase were optimized to obtain the best separation. UV detection was performed at 296 nm. The described method is linear over a range of LOQ--15.0 microg/ml of Z-isomer. The mean recovery of Z-isomer was found to be in the range of 97-99%. The method is simple, rapid, selective, accurate and precise, useful in the quality control of bulk manufacturing.
authors.affiliation: Analytical Research, SMS Pharma Research Center, Sanathnagar, Hyderabad, 500 018, India. phani@smspharma.com
authors.forename: A Phani
authors.initials: AP
authors.lastname: Kumar
authors.forename: V R L
authors.initials: VR
authors.lastname: Ganesh
authors.forename: D V Subba
authors.initials: DV
authors.lastname: Rao
authors.forename: C
authors.initials: C
authors.lastname: Anil
authors.forename: V S
authors.initials: VS
authors.lastname: Hariharakrishnan
mesh_terms: D000935:Antifungal Agents / Q000032:analysis* / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D007093:Imidazoles / Q000032:analysis* / Q000737:chemistry; D011786:Quality Control; D015203:Reproducibility of Results; D013237:Stereoisomerism
chemical_list: D000935:Antifungal Agents; D007093:Imidazoles; C072774:latoconazole