title: A specific phospholipase C activity regulates phosphatidylinositol levels in lung surfactant of patients with acute respiratory distress syndrome.
abstract: Lung surfactant (LS) is a lipid-rich material lining the inside of the lungs. It reduces surface tension at the liquid/air interface and thus, it confers protection of the alveoli from collapsing. The surface-active component of LS is dipalmitoyl-phosphatidylcholine, while anionic phospholipids such as phosphatidylinositol (PtdIns) and primarily phosphatidylglycerol are involved in the stabilization of the LS monolayer. The exact role of PtdIns in this system is not well-understood; however, PtdIns levels change dramatically during the acute respiratory distress syndrome (ARDS) evolution. In this report we present evidence of a phosphoinositide-specific phospholipase C (PI-PLC) activity in bronchoalveolar lavage (BAL) fluid, which may regulate PtdIns levels. Characterization of this extracellular activity showed specificity for PtdIns and phosphatidylinositol 4,5-bisphosphate, sharing the typical substrate concentration-, pH-, and calcium-dependencies with mammalian PI-PLCs. Fractionation of BAL fluid showed that PI-PLC did not co-fractionate with large surfactant aggregates, but it was found mainly in the soluble fraction. Importantly, analysis of BAL samples from control subjects and from patients with ARDS showed that the PI-PLC specific activity was decreased by 4-fold in ARDS samples concurrently with the increase in BAL PtdIns levels. Thus, we have identified for the first time an extracellular PI-PLC enzyme activity that may be acutely involved in the regulation of PtdIns levels in LS.
authors.affiliation: Department of Chemistry, University of Athens, Zografou, 15771 Athens, Greece.
authors.forename: Spyros
authors.initials: S
authors.lastname: Spyridakis
authors.forename: George
authors.initials: G
authors.lastname: Leondaritis
authors.forename: George
authors.initials: G
authors.lastname: Nakos
authors.forename: Marilena E
authors.initials: ME
authors.lastname: Lekka
authors.forename: Dia
authors.initials: D
authors.lastname: Galanopoulou
mesh_terms: D000328:Adult; D000368:Aged; D001992:Bronchoalveolar Lavage Fluid; D002118:Calcium / Q000378:metabolism; D003710:Demography; D005110:Extracellular Space / Q000201:enzymology; D005260:Female; D006801:Humans; D006868:Hydrolysis; D042541:Intracellular Space / Q000201:enzymology; D008297:Male; D008875:Middle Aged; D019269:Phosphatidylinositol 4,5-Diphosphate; D018129:Phosphatidylinositol Phosphates / Q000378:metabolism*; D054801:Phosphoinositide Phospholipase C / Q000378:metabolism*; D011663:Pulmonary Surfactants / Q000378:metabolism*; D012128:Respiratory Distress Syndrome / Q000201:enzymology* / Q000473:pathology; D013379:Substrate Specificity
chemical_list: D019269:Phosphatidylinositol 4,5-Diphosphate; D018129:Phosphatidylinositol Phosphates; D011663:Pulmonary Surfactants; D054801:Phosphoinositide Phospholipase C; D002118:Calcium