title: Frequency and function of circulating invariant NKT cells in autoimmune diabetes mellitus and thyroid diseases in Colombian patients.
abstract: The frequency and functionality of peripheral blood invariant (iNKT) cells and their subsets, as well as other regulatory T-cell subsets, were evaluated in patients with type 1A diabetes mellitus (DM1), Hashimoto's disease, and Graves' disease. In addition to healthy individuals (HC), patients with type 2 diabetes mellitus (DM2) were included as controls because this disease has a different physiopathology. A similar frequency of total iNKT cells, as well as their subsets, existed among HC and the different study groups. Similar results were reported when we compared the frequency of CD4(+)/CD25(high) T cells, CD8(+)/CD28(negative) T cells, and gamma-delta T cells among HC and study groups, whereas patients with DM2 exhibited a higher frequency of CD8(+)/CD28(negative) T cells compared with HC and DM1. Also, patients with DM2 exhibited a lower frequency of CD4(negative) and CD4(+) iNKT cells expressing tumor necrosis factor-alpha (TNF-alpha) than HC. We did not observe significant differences in the frequency of iNKT cells expressing interleukin-4 or interferon-gamma among study groups and controls. Our findings support a normal frequency and function of peripheral blood iNKT cells in different endocrine autoimmune diseases, but an abnormal expression of TNF-alpha by circulating iNKT cells from patients with DM2.
authors.affiliation: Group of Immunovirology, University of Antioquia, Medellin, Colombia.
authors.forename: Alejandro
authors.initials: A
authors.lastname: Roman-Gonzalez
authors.forename: Maria Eugenia
authors.initials: ME
authors.lastname: Moreno
authors.forename: Juan Manuel
authors.initials: JM
authors.lastname: Alfaro
authors.forename: Federico
authors.initials: F
authors.lastname: Uribe
authors.forename: Guillermo
authors.initials: G
authors.lastname: Latorre-Sierra
authors.forename: Maria Teresa
authors.initials: MT
authors.lastname: Rugeles
authors.forename: Carlos Julio
authors.initials: CJ
authors.lastname: Montoya
mesh_terms: D000328:Adult; D003105:Colombia; D003922:Diabetes Mellitus, Type 1 / Q000097:blood* / Q000473:pathology; D003924:Diabetes Mellitus, Type 2 / Q000097:blood / Q000473:pathology; D005260:Female; D005434:Flow Cytometry; D006111:Graves Disease / Q000097:blood / Q000473:pathology; D006801:Humans; D007371:Interferon-gamma / Q000097:blood; D015847:Interleukin-4 / Q000097:blood; D007694:Killer Cells, Natural / Q000378:metabolism* / Q000473:pathology; D018655:Lymphocyte Count; D008297:Male; D008875:Middle Aged; D013959:Thyroid Diseases / Q000097:blood* / Q000473:pathology; D014409:Tumor Necrosis Factor-alpha / Q000097:blood; D055815:Young Adult
chemical_list: D014409:Tumor Necrosis Factor-alpha; D015847:Interleukin-4; D007371:Interferon-gamma