'Injecting' yeast.
Yeast is a powerful genetic model system, but its rigid cell wall has prohibited microinjection. Using microfabricated channels to constrain the fission yeast Schizosaccharomyces pombe, we sheared local regions of individual cells with a piezoelectric unit. The cells remained viable, we detected actin patches in the cell after introduction of fluorescent phalloidin into the medium, and the cytokinetic ring was disrupted after injection of the myosin II inhibitor blebbistatin.
Riveline
Daniel
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Laboratory of Yeast Genetics and Cell Biology, The Rockefeller University, New York, New York, USA. daniel.riveline@ujf-grenoble.fr
Nurse
Paul
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D000199:Actins / Q000378:metabolism; D005456:Fluorescent Dyes / Q000008:administration & dosage; D006576:Heterocyclic Compounds, 4 or More Rings / Q000008:administration & dosage; D008845:Microinjections / Q000295:instrumentation / Q000379:methods*; D008856:Microscopy, Fluorescence; D008858:Microscopy, Phase-Contrast; D010590:Phalloidine / Q000008:administration & dosage; D012568:Schizosaccharomyces / Q000187:drug effects / Q000235:genetics / Q000378:metabolism* / Q000648:ultrastructure*; D029702:Schizosaccharomyces pombe Proteins / Q000378:metabolism
D000199:Actins; D005456:Fluorescent Dyes; D006576:Heterocyclic Compounds, 4 or More Rings; D029702:Schizosaccharomyces pombe Proteins; D010590:Phalloidine; C472645:blebbistatin