Molecular characterization of canine parvovirus strains in Argentina: Detection of the pathogenic variant CPV2c in vaccinated dogs.
PCR amplification with sequence-specific primers was used to detect canine parvovirus (CPV) DNA in 38 rectal swabs from Argentine domestic dogs with symptoms compatible with parvovirus disease. Twenty-seven out of 38 samples analyzed were CPV positive. The classical CPV2 strain was not detected in any of the samples, but nine samples were identified as CPV2a variant and 18 samples as CPV2b variant. Further sequence analysis revealed a mutation at amino acid 426 of the VP2 gene (Asp426Glu), characteristic of the CPV2c variant, in 14 out of 18 of the samples identified initially by PCR as CPV2b. The appearance of CPV2c variant in Argentina might be dated at least to the year 2003. Three different pathogenic CPV variants circulating currently in the Argentine domestic dog population were identified, with CPV2c being the only variant affecting vaccinated and unvaccinated dogs during the year 2008.
Calderon
Marina Gallo
MG
Centro de Virología Animal, Instituto de Ciencia y Tecnología Dr. Cesar Milstein, CONICET, Saladillo 2468, C1440FFX Buenos Aires, Argentina.
Mattion
Nora
N
Bucafusco
Danilo
D
Fogel
Fernando
F
Remorini
Patricia
P
La Torre
Jose
J
D000595:Amino Acid Sequence; D000818:Animals; D001118:Argentina / Q000453:epidemiology; D004283:Dog Diseases / Q000453:epidemiology* / Q000276:immunology / Q000821:virology*; D004285:Dogs; D005260:Female; D008297:Male; D017720:Molecular Epidemiology; D008969:Molecular Sequence Data; D020125:Mutation, Missense; D010322:Parvoviridae Infections / Q000453:epidemiology / Q000276:immunology / Q000662:veterinary* / Q000821:virology; D017993:Parvovirus, Canine / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D016133:Polymerase Chain Reaction; D012007:Rectum / Q000821:virology; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D015678:Viral Structural Proteins / Q000235:genetics; D014765:Viral Vaccines / Q000276:immunology
D015678:Viral Structural Proteins; D014765:Viral Vaccines