Identification of a new pheromone-binding protein in the antennae of a geometrid species and preparation of its antibody to analyze the antennal proteins of moths secreting type II sex pheromone components.
The full-length cDNA sequence of a new pheromone-binding protein (AscrPBP2) was determined from a geometrid moth, Ascotis selenaria cretacea, which secreted a Type II sex pheromone, and an antiserum against its recombinant protein overexpressed in Escherichia coli was prepared. In addition to this antiserum against AscrPBP2, antibodies against AscrPBP1 and general odorant-binding proteins of Bombyx mori were used in Western blotting experiments to analyze the proteins in the antennae of several lepidopteran species secreting Type II sex pheromone components.
Watanabe
Hayaki
H
Graduate School of Bio-Applications and Systems Engineering, Tokyo University of Agriculture and Technology, Koganei, Tokyo, Japan.
Tabunoki
Hiroko
H
Miura
Nami
N
Matsui
Aya
A
Sato
Ryoichi
R
Ando
Tetsu
T
D000595:Amino Acid Sequence; D000818:Animals; D000906:Antibodies / Q000276:immunology; D001483:Base Sequence; D012831:Bombyx; D017931:DNA Primers; D018076:DNA, Complementary; D019476:Insect Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D008969:Molecular Sequence Data; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012724:Sex Attractants / Q000378:metabolism*
D000906:Antibodies; D017931:DNA Primers; D018076:DNA, Complementary; D019476:Insect Proteins; D012724:Sex Attractants