title: [Effect of chemokine receptor 7 small interfering RNA on proliferation and invasion of squamous cell carcinoma of head and neck].
abstract: OBJECTIVE
  To study the effect of chemokine receptor 7 (CCR-7) small interfering RNA (siRNA) on proliferation and invasion of squamous cell carcinoma of head and neck (SCCHN).
  
  
  METHODS
  CCR-7 siRNA was co-transfected into SCCHN cell line PCI-4B using Lipofectamine 2000. CCR-7 protein level was detected by western blotting. SCCHN cell proliferation was detected by MTT, and the change of actin cytoskeleton observed by confocal laser scanning microscope. Transwell assays were used to determine chemotaxis and invasion of SCCHN cells. The activity and nuclear translocation of nuclear factor-kappa B (NF-kappa B) were detected by TransAM NF-kappa B p65 kit and fluorescence microscope respectively.
  
  
  RESULTS
  After CCR-7 siRNA transfection, the protein level of CCR-7 was significantly decreased. The changes induced by CCL-19, including increased proliferation rate, polarized actin polymerization, increased chemotaxis rate and invasion rate, were all abolished by CCR-7 siRNA transfection. CCR-7 siRNA also diminished CCL-19-induced NF-kappaB activation and nuclear translocation.
  
  
  CONCLUSIONS
  CCR-7 siRNA could inhibit expression of CCR-7 and diminish the increased proliferation and invasion of SCCHN induced by CCL-19 in vitro. CCR-7 siRNA may provide a potential treatment strategy for SCCHN.
authors.affiliation: Department of Oral and Maxillofacial Surgery, School of Stomatology, China Medical University, Shenyang 110002, China.
authors.forename: Zhen-Jin
authors.initials: ZJ
authors.lastname: Zhao
authors.forename: Fa-Yu
authors.initials: FY
authors.lastname: Liu
authors.forename: Chang-Fu
authors.initials: CF
authors.lastname: Sun
mesh_terms: D002294:Carcinoma, Squamous Cell / Q000235:genetics / Q000473:pathology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D006258:Head and Neck Neoplasms / Q000235:genetics / Q000473:pathology*; D006801:Humans; D009361:Neoplasm Invasiveness; D034741:RNA, Small Interfering; D054400:Receptors, CCR7 / Q000235:genetics*; D051996:Transcription Factor RelA / Q000378:metabolism
chemical_list: C515370:CCR7 protein, human; C498143:RELA protein, human; D034741:RNA, Small Interfering; D054400:Receptors, CCR7; D051996:Transcription Factor RelA