Super-resolution fluorescence microscopy.
Achieving a spatial resolution that is not limited by the diffraction of light, recent developments of super-resolution fluorescence microscopy techniques allow the observation of many biological structures not resolvable in conventional fluorescence microscopy. New advances in these techniques now give them the ability to image three-dimensional (3D) structures, measure interactions by multicolor colocalization, and record dynamic processes in living cells at the nanometer scale. It is anticipated that super-resolution fluorescence microscopy will become a widely used tool for cell and tissue imaging to provide previously unobserved details of biological structures and processes.
Huang
Bo
B
Howard Hughes Medical Institute, Harvard University, Cambridge, MA 02138, USA. bohuang@fas.harvard.edu
Bates
Mark
M
Zhuang
Xiaowei
X
D003584:Cytological Techniques; D006801:Humans; D021621:Imaging, Three-Dimensional; D008856:Microscopy, Fluorescence / Q000295:instrumentation / Q000379:methods*