Isolation and fluorescence spectrum of cardiac myosin from pig heart.
Cardiac myosin is the most abundant and bioactive protein presented in heart tissue. In this work, a simple method was described for preparation of high purity cardiac myosin for further research. With the use of a domestic pig left ventricle as the starting tissue, this method yielded 4.5 mg myosin per gram of wet tissue with a specific activity of 0.293 U/mg. The purification factor was 4.9-fold with an activity recovery of 42.1%. In addition, the investigation of fluorescence spectrum shown that the emission intensity of cardiac myosin has a dramatic decrease under the influence of an acidic environment or at temperatures higher than 30 degrees C, which could influence the normal physiological function of cardiac myosin.
Liu
Yang
Y
School of Life Science and Technology, Wuhan University of Science and Technology Zhongnan Branch, 430223 Wuhan, Hubei, People's Republic of China. znmdly@sina.com
Li
Buhai
B
Sun
Xiaomei
X
Lin
Aihua
A
D000645:Ammonium Sulfate / Q000737:chemistry; D000818:Animals; D024722:Cardiac Myosins / Q000737:chemistry* / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D006352:Heart Ventricles / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D009206:Myocardium / Q000737:chemistry*; D013050:Spectrometry, Fluorescence / Q000379:methods*; D013552:Swine; D013696:Temperature; D014961:X-Ray Diffraction
D024722:Cardiac Myosins; D000645:Ammonium Sulfate