title: Genetic analysis of aquabirnaviruses isolated from wild fish reveals occurrence of natural reassortment of infectious pancreatic necrosis virus.
abstract: In this study, we report the sequencing of the whole genome [including the 5' and 3' non-coding regions (NCR) of both segments A and B] of seven birnavirus strains isolated from wild fish from the Flemish Cap (FC) fishery at Newfoundland, Canada. From analysis and comparison of the sequences, most of the FC isolates clustered with the North American reference strains West Buxton (WB), Dry Mill and Jasper. One strain was included in the same genotype as the European strain Ab. In addition, at least in one case cohabitation of both type strains in an individual fish was demonstrated. These results clearly suggest the acquisition of the viruses from two different sources. The prevalence of the American type is easily explained by the close proximity of this fishing bank to the American coast whereas, although surprising, the presence of the European type strain could be because of migration of fish from European waters. In one strain, segment A and B sequences were typed differently (WB and Ab, respectively). These findings indicate natural reassortment between two strains of aquabirnaviruses in a host.
authors.affiliation: Unidade de Ictiopatoloxía, Dpt Microbioloxía e Parasitoloxía, Instituto de Acuicultura, Universidade de Santiago de Compostela, Santiago de Compostela, Spain.
authors.forename: I
authors.initials: I
authors.lastname: Romero-Brey
authors.forename: I
authors.initials: I
authors.lastname: Bandín
authors.forename: J M
authors.initials: JM
authors.lastname: Cutrín
authors.forename: V N
authors.initials: VN
authors.lastname: Vakharia
authors.forename: C P
authors.initials: CP
authors.lastname: Dopazo
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D019201:Aquabirnavirus / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D016000:Cluster Analysis; D017931:DNA Primers / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D005399:Fishes / Q000821:virology*; D008969:Molecular Sequence Data; D009523:Newfoundland and Labrador; D010802:Phylogeny; D016865:Reassortant Viruses / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D013045:Species Specificity
chemical_list: D017931:DNA Primers; D018076:DNA, Complementary