title: 7-Oxoorobanchyl acetate and 7-Oxoorobanchol as germination stimulants for root parasitic plants from flax (Linum usitatissimum).
abstract: Germination stimulants for root parasitic plants produced by flax (Linum usitatissimum L.) were purified and characterized. The root exudate of flax contained at least 8 active fractions, and liquid chromatography-tandem mass spectrometry (LC-MS/MS) and gas chromatography mass spectrometry (GC-MS) analyses suggested that there were 6 strigolactones. Two of them were identified as orobanchol and orobanchyl acetate by comparing NMR and GC-MS and LC-MS/MS data with those of synthetic standards. One of the two novel strigolactones was purified and determined as 7-oxoorobanchyl acetate [((3aS,4S,8bS,E)-8,8-dimethyl-3-(((R)-4-methyl-5-oxo-2,5-dihydrofuran-2-yloxy)methylene)-2,7-dioxo-3,3a,4,5,6,7,8,8b-octahydro-2H-indeno[1,2-b]furan-4-yl acetate) by 1D and 2D NMR spectroscopic, and ESI- and EI-MS spectrometric analyses. The other one was also purified and identified as 7-oxoorobanchol. The remaining two compounds could not been characterized due to their scarcity.
authors.affiliation: Weed Science Center, Utsunomiya University, Utsunomiya, Japan.
authors.forename: Xiaonan
authors.initials: X
authors.lastname: Xie
authors.forename: Kaori
authors.initials: K
authors.lastname: Yoneyama
authors.forename: Jun-ya
authors.initials: JY
authors.lastname: Kurita
authors.forename: Yuta
authors.initials: Y
authors.lastname: Harada
authors.forename: Yoichi
authors.initials: Y
authors.lastname: Yamada
authors.forename: Yasutomo
authors.initials: Y
authors.lastname: Takeuchi
authors.forename: Koichi
authors.initials: K
authors.lastname: Yoneyama
mesh_terms: D002853:Chromatography, Liquid; D019597:Flax / Q000737:chemistry*; D005663:Furans / Q000494:pharmacology*; D008401:Gas Chromatography-Mass Spectrometry; D018525:Germination / Q000187:drug effects*; D007192:Indenes / Q000494:pharmacology*; D009682:Magnetic Resonance Spectroscopy; D010936:Plant Extracts / Q000494:pharmacology*; D018517:Plant Roots / Q000187:drug effects*; D053719:Tandem Mass Spectrometry
chemical_list: C544056:7-oxoorobanchol; C544055:7-oxoorobanchyl acetate; D005663:Furans; D007192:Indenes; D010936:Plant Extracts