Resveratrol-loaded polymeric micelles protect cells from Abeta-induced oxidative stress.
Resveratrol has been reported to protect several types of cells against beta-amyloid peptide (Abeta) toxicity by scavenging reactive oxygen species (ROS) and inactivating caspase-3. However, other studies found that long-term treatment with resveratrol inhibited cells by inducing ROS generation and activating caspase-3. In the current report, a 48-h incubation of resveratrol at the concentrations of 5 and 10 microM significantly attenuated the viability of PC12 cells and a 12-h pre-incubation of resveratrol did not protect PC12 cells against Abeta exposure (even further inhibited PC12 cells at the concentrations of 10 microM) by acting as a pro-oxidant. Due to the lipophilicity of resveratrol, resveratrol-loaded polymeric micelles basing on amphiphilic block copolymer were developed. Then, the effects of resveratrol-loaded polymeric micelles on the viability and Abeta protection of PC12 cells were investigated. At the equivalent concentrations of 5 and 10 microM resveratrol, a 48-h incubation of resveratrol-loaded nanoparticles did not show toxicity to cells, while 12-h pre-incubation of resveratrol-loaded nanoparticles protected PC12 cells from Abeta-induced damage in a dose dependent manner (1-10 microM) by attenuating intracellular oxidative stress and caspase-3 activity. Further investigations are absolutely needed to evaluate the feasibility and advantages of in vivo applications of resveratrol-loaded nanoparticles.
Lu
Xiaowei
X
Department of Neurology, The First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, PR China.
Ji
Chenbo
C
Xu
Huae
H
Li
Xiaolin
X
Ding
Haixia
H
Ye
Min
M
Zhu
Zhenshu
Z
Ding
Dan
D
Jiang
Xiqun
X
Ding
Xinsheng
X
Guo
Xirong
X
D016229:Amyloid beta-Peptides / Q000633:toxicity*; D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage / Q000494:pharmacology*; D053148:Caspase 3 / Q000378:metabolism; D002470:Cell Survival / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D008823:Micelles; D053758:Nanoparticles; D018384:Oxidative Stress / Q000187:drug effects*; D016716:PC12 Cells; D011108:Polymers / Q000737:chemistry; D051381:Rats; D017382:Reactive Oxygen Species / Q000378:metabolism; D000077185:Resveratrol; D013267:Stilbenes / Q000008:administration & dosage / Q000009:adverse effects / Q000494:pharmacology*
D016229:Amyloid beta-Peptides; D000975:Antioxidants; D008823:Micelles; D011108:Polymers; D017382:Reactive Oxygen Species; D013267:Stilbenes; D053148:Caspase 3; D000077185:Resveratrol