title: HIV-1 interactions with cells: from viral binding to cell-cell transmission.
abstract: Characterization of HIV-1 interactions with host cells is critical for cell biology studies of HIV-1. This unit describes a set of methods and protocols to perform quantitative assays of HIV-1 binding, internalization, infection, and cell-cell transmission. The protocols include: (1) generating infectious single-cycle or replication-competent HIV-1 stocks, (2) an HIV-1 binding and internalization assay, (3) HIV-1 infection of target cells and quantification of viral infection, and (4) HIV-1 cell-cell transmission assays. These functional assays provide useful tools to quantitatively study HIV-1 infection and viral transmission.
authors.affiliation: Department of Microbiology and Molecular Genetics, Medical College of Wisconsin, Milwaukee, Wisconsin, USA.
authors.forename: Alicia M
authors.initials: AM
authors.lastname: Janas
authors.forename: Li
authors.initials: L
authors.lastname: Wu
mesh_terms: D000818:Animals; D002460:Cell Line; D015658:HIV Infections / Q000473:pathology / Q000821:virology*; D015497:HIV-1 / Q000472:pathogenicity / Q000502:physiology*; D054884:Host-Pathogen Interactions / Q000502:physiology; D006801:Humans; D008156:Luciferases; D017751:Safety Management; D014773:Virology / Q000379:methods*; D014774:Virulence; D037521:Virulence Factors; D053585:Virus Attachment; D014779:Virus Replication
chemical_list: D037521:Virulence Factors; D008156:Luciferases