Determination of Tenacissoside A in rat plasma by liquid chromatography-tandem mass spectrometry method and its application to pharmacokinetic study.
A sensitive and specific liquid chromatography-tandem mass spectrometry method was developed and validated for the first time for the estimation of Tenacissoside A in the rats' plasma, which is the major active constituent in Marsdenia tenacissima. Tenacissoside A was extracted from the rats' plasma by using liquid-liquid extraction (LLE), medroxyprogesterone acetate was used as the internal standard. An Alltech C18 column (250 mm x 4.6mm, 5 microm) was used to provide chromatographic separation by detection with mass spectrometry operating in selected ion monitoring (SIM) mode. The method was validated over the concentration range of 1-250 ng/mL for Tenacissoside A. The precisions within and between-batch (CV%) were both less than 15% and accuracy ranged from 90 to 102%. The lower limit of quantification was 1 ng/mL and extraction recovery was 88.3% on average. The validated method was used to study the pharmacokinetic profile of Tenacissoside A in rat after administration.
Zhao
Luhua
L
Key Laboratory of Drug Quality Control and Pharmacovigilance (China Pharmaceutical University), Ministry of Education, Nanjing, Jiangsu 210009, China.
Xiang
Bingren
B
Chen
Jing
J
Tan
Xiying
X
Wang
Dawei
D
Chen
Daofeng
D
D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000032:analysis* / Q000737:chemistry / Q000493:pharmacokinetics; D008297:Male; D031101:Marsdenia / Q000737:chemistry*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013256:Steroids / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics; D053719:Tandem Mass Spectrometry / Q000379:methods*
D004365:Drugs, Chinese Herbal; D013256:Steroids; C531262:tenacissimoside A