Biliary tract injury caused by different relative warm ischemia time in liver transplantation in rats.
BACKGROUND
There is a controversy over the degree of liver and biliary injury caused by the period of secondary warm ischemia. A liver autotransplantation model was adopted because it excludes the effects of infection and immunological rejection on bile duct injury. This study was undertaken to assess biliary tract injury caused by relative warm ischemia (secondary warm ischemia time in the biliary tract) and reperfusion.


METHODS
One hundred and two rats were randomly divided into 5 groups: group I (control); groups II to V, relative warm ischemia times of 0 minute, 30 minutes, 1 hour and 2 hours. In addition to the levels of serum alkaline phosphatase, and total bilirubin, pathomorphology assessment and TUNEL assay were performed to evaluate biliary tract damage.


RESULTS
Under the conditions that there were no significant differences in warm ischemia time, cold perfusion time and anhepatic phase, group comparisons showed statistically significant differences. The least injury occurred in group II (portal vein and hepatic artery reperfused simultaneously) but the most severe injury occurred in group V (biliary tract relative warm ischemia time 2 hours).


CONCLUSIONS
Relative warm ischemia is one of the factors that result in bile duct injury, and the relationship between relative warm ischemia time the bile injury degree is time-dependent. Simultaneous arterial and portal reperfusion is the best choice to avoid the bile duct injury caused by relative warm ischemia.
Zhao
Hong-Feng
HF
Department of Hepatobiliary Surgery, Nanfang Hospital, Southern Medical University, Guangzhou, China.
Zhang
Guo-Wei
GW
Zhou
Jie
J
Lin
Jian-Hua
JH
Cui
Zhong-Lin
ZL
Li
Xiang-Hong
XH
D000469:Alkaline Phosphatase / Q000097:blood; D000818:Animals; D017209:Apoptosis; D001652:Bile Ducts / Q000473:pathology / Q000503:physiopathology; D001659:Biliary Tract / Q000098:blood supply*; D001663:Bilirubin / Q000097:blood; D005260:Female; D006499:Hepatic Artery; D016031:Liver Transplantation; D008297:Male; D011169:Portal Vein; D051381:Rats; D017207:Rats, Sprague-Dawley; D015424:Reperfusion; D015427:Reperfusion Injury / Q000209:etiology*; D014182:Transplantation, Autologous; D052096:Warm Ischemia / Q000009:adverse effects*
D000469:Alkaline Phosphatase; D001663:Bilirubin