3-(10'-Phenothiazinyl)propane-1-sulfonate is a potent enhancer of soybean peroxidase-induced chemiluminescence.
3-(10'-Phenothiazinyl)propane-1-sulfonate (SPTZ) was shown to be a potent enhancer of soybean peroxidase (SbP)-induced chemiluminescence. To the best of our knowledge, SPTZ is the first enhancer of SbP to be discovered. Optimal conditions for SbP-catalyzed oxidation of luminol in the presence of SPTZ were determined. The SbP-SPTZ system showed better sensitivity and a lower detection limit (LDL) with respect to the horseradish peroxidase-4-iodophenol system traditionally used in chemiluminescent enzyme-linked immunosorbent assay (ELISA). The addition of 4-morpholinopyridine (MORP) to the SbP-SPTZ system improved its analytical parameters by decreasing the LDL of SbP to 0.03pM. These results open up very promising perspectives for using the SbP-SPTZ-MORP system in ultrasensitive immunoassay.
Vdovenko
Marina M
MM
Department of Chemical Enzymology, Faculty of Chemistry, M.V. Lomonosov Moscow State University, 119991 Moscow, Russia.
Ciana
Leopoldo Della
LD
Sakharov
Ivan Yu
IY
D017738:Alkanesulfonic Acids / Q000737:chemistry*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D007700:Kinetics; D008163:Luminescent Measurements / Q000379:methods*; D015394:Molecular Structure; D009195:Peroxidase / Q000378:metabolism; D012680:Sensitivity and Specificity; D013025:Glycine max / Q000201:enzymology; D013379:Substrate Specificity; D013843:Thiazines / Q000737:chemistry*
C543492:3-(10'-Phenothiazinyl)propane-1-sulfonate; D017738:Alkanesulfonic Acids; D013843:Thiazines; D009195:Peroxidase