Deinococcus gobiensis sp. nov., an extremely radiation-resistant bacterium.
A Gram-positive, non-motile, spherical, red-pigmented and facultatively anaerobic bacterium, designated strain I-0(T), was isolated from a sand sample of the Gobi desert in Xinjiang Autonomous Region, China. Phylogenetic analysis based on 16S rRNA gene sequences indicated that this isolate represents a novel member of the genus Deinococcus, with low sequence similarities (<94 %) to recognized Deinococcus species. The major cellular fatty acids were C(16 : 1)omega7c and C(16 : 0). Its polar lipid profile contained several unidentified glycolipids, phosphoglycolipids, phospholipids, pigments and an aminophospholipid. The peptidoglycan type was Orn-Gly(2) (A3beta) and the predominant respiratory quinone was MK-8. The DNA G+C content was 65.4 mol%. DNA-DNA relatedness between strain I-0(T) and Deinococcus radiodurans ACCC 10492(T) was 37 %. The strain was shown to be extremely resistant to gamma radiation (>15 kGy) and UV light (>600 J m(-2)). On the basis of the phylogenetic, chemotaxonomic and phenotypic data presented, strain I-0(T) represents a novel species of the genus Deinococcus, for which the name Deinococcus gobiensis sp. nov. is proposed. The type strain is I-0(T) (=DSM 21396(T) =CGMCC 1.7299(T)).
Yuan
Menglong
M
Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, PR China.
Zhang
Wei
W
Dai
Shiming
S
Wu
Jing
J
Wang
Yingdian
Y
Tao
Tianshen
T
Chen
Ming
M
Lin
Min
M
D015373:Bacterial Typing Techniques; D001482:Base Composition; D002681:China; D004269:DNA, Bacterial / Q000032:analysis; D004275:DNA, Ribosomal / Q000032:analysis; D034301:Deinococcus / Q000145:classification* / Q000302:isolation & purification / Q000502:physiology / Q000528:radiation effects*; D003889:Desert Climate; D005720:Gamma Rays; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010641:Phenotype; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012822:Silicon Dioxide; D012988:Soil Microbiology; D013045:Species Specificity; D014466:Ultraviolet Rays
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D012822:Silicon Dioxide