Effect of acylethanolamides on lipid peroxidation and paraoxonase activity.
N-acylethanolamides (NAEs) are hydrophobic molecules synthesized in many tissues. An increase in the plasma levels of NAEs has been observed in human diseases. Previous studies have suggested that NAEs could exert a protective effect against oxidative stress. Aim of the study was to investigate whether NAEs (oleoylethanolamide, palmitoylethanolamide and anandamide), differing for acyl chain length and unsaturation, exert a protective role against plasma lipid peroxidation triggered by incubation with Cu2+2 or AAPH (2,2'-azobis(2-amidinopropane) dihydrochloride). Moreover, we investigated the effect of NAEs on the activity of HDL-associated paraoxonase (PON1), an enzyme involved in the antioxidant end anti-inflammatory role of human high density lipoproteins (HDL). The results demonstrated that the NAEs protect plasma lipids and PON1 activity against AAPH and/or copper-induced oxidation.
Zolese
Giovanna
G
Dipartimento di Biochimica, Biologia e Genetica, Università Politecnica delle Marche, Ancona, Italy.
Bacchetti
Tiziana
T
Masciangelo
Simona
S
Ragni
Letizia
L
Ambrosi
Simona
S
Ambrosini
Annarina
A
Marini
Milvia
M
Ferretti
Gianna
G
D000328:Adult; D000577:Amides; D000578:Amidines / Q000494:pharmacology; D001095:Arachidonic Acids / Q000494:pharmacology*; D043303:Aryldialkylphosphatase / Q000097:blood* / Q000187:drug effects; D063388:Endocannabinoids; D004983:Ethanolamines; D006801:Humans; D015227:Lipid Peroxidation / Q000187:drug effects*; D008875:Middle Aged; D009829:Oleic Acids / Q000494:pharmacology*; D010169:Palmitic Acids / Q000494:pharmacology*; D053284:Polyunsaturated Alkamides / Q000494:pharmacology*
D000577:Amides; D000578:Amidines; D001095:Arachidonic Acids; D063388:Endocannabinoids; D004983:Ethanolamines; D009829:Oleic Acids; D010169:Palmitic Acids; D053284:Polyunsaturated Alkamides; C488250:oleoylethanolamide; C005958:palmidrol; C046728:2,2'-azobis(2-amidinopropane); D043303:Aryldialkylphosphatase; C078814:anandamide