title: Negligible expression of arsenic in some commercially available brands of Portland cement and mineral trioxide aggregate.
abstract: BACKGROUND
  This study was designed aiming to determine and compare the amount of arsenic in some brands of mineral trioxide aggregate (MTA) and Portland cement.
  
  
  METHODS
  In the present study, arsenic species (As[III], As[V], and dimethylarsinic acid) were separated by high-performance liquid chromatography (HPLC) using a strong anion exchange column and converted into arsines by online HG. The instrumental coupling, HPLC-HG-AFS, was applied to 0.2 g of each cement that was prior digested in a solution of HCl, HNO(3), and HBF(4). Data were expressed as a part per million, and the preliminary analysis of the raw pooled data revealed a bell-shaped distribution. Statistical analysis was performed using one-way analysis of variance for multiple comparisons.
  
  
  RESULTS
  In all chromatograms obtained, only type III arsenic could be detected. The minimum amount of arsenic was detected in samples of white MTA ProRoot (3.3 x 10-4) and the maximum in the samples MTA Bio Angelus (Angelus, Londrina, PR, Brazil) (8.6 x 10-4). In the Gray MTA (Angelus), gray ProRoot MTA (Tulsa/Dentsply, Tulsa, OK) and CP Juntalider (Brasilatex Ltda, Diadema, SP, Brazil) did not detect any trace of arsenic. The values of arsenic found in CP Irajazinho (Votorantim Cimentos, Rio Branco, SP, Brazil) and white MTA Angelus were intermediaries to minimum and maximum values. The nonparametric test Kruskal-Wallis showed statistically similar results among all cements tested (p > 0.5).
  
  
  CONCLUSIONS
  Overall, the present study showed that all cements showed insignificant amounts of type III arsenic as well as no trace of arsenic DMA and type V could be detected.
authors.affiliation: Department of Endodontics, Rio de Janeiro State University, Rio de Janeiro, Brazil. endogus@gmail.com
authors.forename: Gustavo
authors.initials: G
authors.lastname: De-Deus
authors.forename: Maria Claudia Brandão
authors.initials: MC
authors.lastname: de Souza
authors.forename: Rivail Antonio
authors.initials: RA
authors.lastname: Sergio Fidel
authors.forename: Sandra Rivera
authors.initials: SR
authors.lastname: Fidel
authors.forename: Reinaldo Calixto
authors.initials: RC
authors.lastname: de Campos
authors.forename: Aderval S
authors.initials: AS
authors.lastname: Luna
mesh_terms: D017607:Aluminum Compounds / Q000737:chemistry*; D001151:Arsenic / Q000032:analysis*; D017610:Calcium Compounds / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D003738:Dental Cements / Q000737:chemistry*; D004338:Drug Combinations; D007202:Indicators and Reagents; D010087:Oxides / Q000737:chemistry*; D012387:Root Canal Filling Materials / Q000737:chemistry*; D017640:Silicates / Q000737:chemistry*
chemical_list: D017607:Aluminum Compounds; D017610:Calcium Compounds; D003738:Dental Cements; D004338:Drug Combinations; D007202:Indicators and Reagents; D010087:Oxides; D012387:Root Canal Filling Materials; D017640:Silicates; C472247:accelerated Portland cement; C086631:mineral trioxide aggregate; D001151:Arsenic