Ultraviolet and tandem mass spectrometry for simultaneous quantification of 21 pivotal metabolites in plasma from patients with diabetic nephropathy.
A sensitive and specific method was developed for simultaneous determination of 21 compounds related to the diabetic nephropathy (DN) in a single analysis using high-performance liquid chromatography coupled to ultraviolet and tandem mass spectrometry (HPLC-UV/MS/MS) in human plasma. With retention times and MS/MS for peak identification, both UV and MS detectors were used for quantification. Calibration curves suitable for the analysis of plasma were linear (r(2)>0.998) with limits of detection (LOD) from 10 to 1000 ng/mL. Intraday relative standard deviation (R.S.D.) and interday R.S.D. were both lower than 15%. With the case and control study, we found five potential biomarkers of DN, including adenosine, inosine, uric acid, xanthine and creatinine.
Xia
Jian-Fei
JF
School of Pharmaceutics, East-China University of Science & Technology, China.
Liang
Qiong-Lin
QL
Liang
Xiao-Ping
XP
Wang
Yi-Ming
YM
Hu
Ping
P
Li
Ping
P
Luo
Guo-An
GA
D000368:Aged; D015415:Biomarkers / Q000097:blood; D016022:Case-Control Studies; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003928:Diabetic Nephropathies / Q000097:blood*; D006801:Humans; D055442:Metabolome; D055432:Metabolomics / Q000379:methods*; D008875:Middle Aged; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet / Q000379:methods*; D053719:Tandem Mass Spectrometry / Q000379:methods*
D015415:Biomarkers