title: High prevalence of human papillomavirus type 58 in patients with cervical pre-malignant lesions in southern Brazil.
abstract: Cervical cancer is the second most common type of cancer in women worldwide. Several human papillomavirus (HPV) genotypes, sexual behavior, and socioeconomic profile represent major risk factors for the development of this carcinoma. Cervical invasive cancer is preceded by cellular abnormalities that can be identified by cytological or histological exams. In order to determine the prevalence and genotypes of HPV in women with abnormal cytology or histopathology, cervical cell samples from 256 patients were evaluated for the presence of HPV/DNA by polymerase chain reaction (PCR), followed by virus genotyping by restriction fragment length polymorphism (RFLP). A total of 113 samples (51.2%) were HPV/DNA positive. Viral genotyping showed that the most prevalent genotypes were HPV 16 (34.7%) and 58 (13.8%), followed by HPV 33 (9.72%), 11 (8.33%), 18 (5.55%), 53 (5.55%), and 6 (4.2%). Four samples (5.55%) exhibited multiple infections due to the great similarity of socioeconomic characteristics and sexual behavior of HPV positive women, it was not possible to establish a risk profile for female HPV infection.
authors.affiliation: Laboratório de Diagnóstico Molecular, Instituto de Biotecnologia, Universidade de Caxias do Sul, Caxias do Sul, Rio Grande do Sul, Brazil.
authors.forename: Suelen
authors.initials: S
authors.lastname: Paesi
authors.forename: Eduardo Pretto
authors.initials: EP
authors.lastname: Serafini
authors.forename: Fernanda
authors.initials: F
authors.lastname: Barea
authors.forename: Sonia Regina Cabral
authors.initials: SR
authors.lastname: Madi
authors.forename: Sergio
authors.initials: S
authors.lastname: Echeverrigaray
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D001938:Brazil / Q000453:epidemiology; D002584:Cervix Uteri / Q000166:cytology* / Q000821:virology*; D016172:DNA Fingerprinting; D004279:DNA, Viral / Q000235:genetics; D005260:Female; D005838:Genotype; D006801:Humans; D008875:Middle Aged; D027383:Papillomaviridae / Q000145:classification* / Q000302:isolation & purification*; D030361:Papillomavirus Infections / Q000453:epidemiology* / Q000821:virology*; D012150:Polymorphism, Restriction Fragment Length; D015995:Prevalence; D012307:Risk Factors; D055815:Young Adult
chemical_list: D004279:DNA, Viral