title: Crystallization and preliminary X-ray studies of the N-domain of the Wilson disease associated protein.
abstract: Wilson disease associated protein (ATP7B) is essential for copper transport in human cells. Mutations that affect ATP7B function result in Wilson's disease, a chronic copper toxicosis. Disease-causing mutations within the N-domain of ATP7B (WND) are known to disrupt ATP binding, but a high-resolution X-ray structure of the ATP-binding site has not been reported. The N-domain was modified to delete the disordered loop comprising residues His1115-Asp1138 (WNDDelta(1115-1138)). Unlike the wild-type N-domain, WNDDelta(1115-1138) formed good-quality crystals. Synchrotron diffraction data have been collected from WNDDelta(1115-1138) at the Canadian Light Source. A native WNDDelta(1115-1138) crystal diffracted to 1.7 A resolution and belonged to space group P4(2)2(1)2, with unit-cell parameters a = 39.2, b = 39.2, c = 168.9 A. MAD data were collected to 2.7 A resolution from a SeMet-derivative crystal with unit-cell parameters a = 38.4, b = 38.4, c = 166.7 A. The WNDDelta(1115-1138) structure is likely to be solved by phasing from multiwavelength anomalous diffraction (MAD) experiments.
authors.affiliation: Department of Biochemistry, University of Saskatchewan, Saskatoon, Saskatchewan, S7N 5E5, Canada.
authors.forename: Lili
authors.initials: L
authors.lastname: Liu
authors.forename: Christopher
authors.initials: C
authors.lastname: O'Grady
authors.forename: Sean A
authors.initials: SA
authors.lastname: Dalrymple
authors.forename: Lata
authors.initials: L
authors.lastname: Prasad
authors.forename: Oleg Y
authors.initials: OY
authors.lastname: Dmitriev
authors.forename: Louis T J
authors.initials: LT
authors.lastname: Delbaere
mesh_terms: D000251:Adenosine Triphosphatases / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000378:metabolism; D000595:Amino Acid Sequence; D001665:Binding Sites; D027682:Cation Transport Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D017124:Conserved Sequence; D003300:Copper / Q000737:chemistry* / Q000378:metabolism; D000073840:Copper-Transporting ATPases; D003460:Crystallization; D018360:Crystallography, X-Ray; D003625:Data Collection; D004926:Escherichia coli / Q000235:genetics; D006527:Hepatolenticular Degeneration / Q000235:genetics; D006801:Humans; D008958:Models, Molecular; D008969:Molecular Sequence Data; D019906:Nuclear Magnetic Resonance, Biomolecular; D011485:Protein Binding; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D013223:Statistics as Topic; D017356:Synchrotrons; D014169:Transformation, Bacterial; D014961:X-Ray Diffraction
chemical_list: D027682:Cation Transport Proteins; D003300:Copper; D000255:Adenosine Triphosphate; D000251:Adenosine Triphosphatases; C000612464:ATP7B protein, human; D000073840:Copper-Transporting ATPases