title: Gastroprotective activity of isopulegol on experimentally induced gastric lesions in mice: investigation of possible mechanisms of action.
abstract: The present study investigated whether isopulegol, a monoterpene present in essential oils of several aromatic plants, would be able to promote some gastroprotective effect and also verified the possible mechanisms involved in this action. For this study, ethanol- and indomethacin-induced gastric ulcer models in mice and histopathological assessment were used. The roles of NO, sulfhydryls (glutathione, GSH), ATP-sensitive K(+) channels (K(ATP) channels), and prostaglandins were also investigated. Isopulegol exhibited a dose-related gastroprotective effect against ethanol-induced lesions, while the pretreatment with glibenclamide and indomethacin [but not with N(G)-nitro-L-arginine methyl ester] were able to reverse this action. The pretreatment with isopulegol also restored GSH levels to normal levels and exhibited dose-related gastroprotective effect against indomethacin-induced ulcer. The results suggested that isopulegol presents significant gastroprotective effects in both ethanol- and indomethacin-induced ulcer models, which appear to be mediated, at least in part, by endogenous prostaglandins, K(ATP) channel opening, and antioxidant properties.
authors.affiliation: Department of Physiology and Pharmacology, Faculty of Medicine, Federal University of Ceará, Rua Cel. Nunes de Melo 1127, Fortaleza, Brazil.
authors.forename: Maria Izabel Gomes
authors.initials: MI
authors.lastname: Silva
authors.forename: Brinell Arcanjo
authors.initials: BA
authors.lastname: Moura
authors.forename: Manuel Rufino de Aquino
authors.initials: MR
authors.lastname: Neto
authors.forename: Adriana da Rocha
authors.initials: Ada R
authors.lastname: Tomé
authors.forename: Nayrton Flávio Moura
authors.initials: NF
authors.lastname: Rocha
authors.forename: Alyne Mara Rodrigues
authors.initials: AM
authors.lastname: de Carvalho
authors.forename: Danielle Silveira
authors.initials: DS
authors.lastname: Macêdo
authors.forename: Silvânia Maria Mendes
authors.initials: SM
authors.lastname: Vasconcelos
authors.forename: Damião Pergentino
authors.initials: DP
authors.lastname: de Sousa
authors.forename: Glauce Socorro de Barros
authors.initials: GS
authors.lastname: Viana
authors.forename: Francisca Cléa Florenço
authors.initials: FC
authors.lastname: de Sousa
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage / Q000494:pharmacology*; D000081005:Cyclohexane Monoterpenes; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D000431:Ethanol / Q000633:toxicity; D005978:Glutathione / Q000187:drug effects / Q000378:metabolism; D007213:Indomethacin / Q000633:toxicity; D054086:KATP Channels / Q000378:metabolism; D008297:Male; D051379:Mice; D011453:Prostaglandins / Q000378:metabolism; D013276:Stomach Ulcer / Q000139:chemically induced / Q000517:prevention & control*; D013729:Terpenes / Q000008:administration & dosage / Q000494:pharmacology*
chemical_list: D000975:Antioxidants; D000081005:Cyclohexane Monoterpenes; D054086:KATP Channels; D011453:Prostaglandins; D013729:Terpenes; D000431:Ethanol; C409816:isopulegol; D005978:Glutathione; D007213:Indomethacin