title: Comparative sequence analysis of the SALT OVERLY SENSITIVE1 orthologous region in Thellungiella halophila and Arabidopsis thaliana.
abstract: To provide a framework for studies to understand the contribution of SALT OVERLY SENSITIVE1 (SOS1) to salt tolerance in Thellungiella halophila, we sequenced and annotated a 193-kb T. halophila BAC containing a putative SOS1 locus (ThSOS1) and compared the sequence to the orthologous 146-kb region of the genome of its salt-sensitive relative, Arabidopsis thaliana. Overall, the two sequences were colinear, but three major expansion/contraction regions in T. halophila were found to contain five Long Terminal Repeat retrotransposons, MuDR DNA transposons and intergenic sequences that contribute to the 47.8-kb size variation in this region of the genome. Twenty-seven genes were annotated in the T. halophila BAC including the putative ThSOS1 locus. ThSOS1 shares gene structure and sequence with A. thaliana SOS1 including 11 predicted transmembrane domains and a cyclic nucleotide-binding domain; however, different patterns of Simple Sequence Repeats were found within a 540-bp region upstream of SOS1 in the two species.
authors.affiliation: Department of Plant Sciences, University of Arizona, Tucson, 85721-0036, USA.
authors.forename: Gyoungju
authors.initials: G
authors.lastname: Nah
authors.forename: Christopher L
authors.initials: CL
authors.lastname: Pagliarulo
authors.forename: Peter G
authors.initials: PG
authors.lastname: Mohr
authors.forename: Meizhong
authors.initials: M
authors.lastname: Luo
authors.forename: Nick
authors.initials: N
authors.lastname: Sisneros
authors.forename: Yeisoo
authors.initials: Y
authors.lastname: Yu
authors.forename: Kristi
authors.initials: K
authors.lastname: Collura
authors.forename: Jennifer
authors.initials: J
authors.lastname: Currie
authors.forename: Jose Luis
authors.initials: JL
authors.lastname: Goicoechea
authors.forename: Rod A
authors.initials: RA
authors.lastname: Wing
authors.forename: Karen S
authors.initials: KS
authors.lastname: Schumaker
mesh_terms: D017360:Arabidopsis / Q000235:genetics*; D029681:Arabidopsis Proteins; D019607:Brassicaceae / Q000235:genetics*; D022202:Chromosomes, Artificial, Bacterial; D003001:Cloning, Molecular; D004251:DNA Transposable Elements; D019143:Evolution, Molecular; D018745:Genome, Plant; D018598:Minisatellite Repeats; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000235:genetics*; D055049:Salt Tolerance; D017923:Sodium-Hydrogen Exchangers / Q000235:genetics*
chemical_list: D029681:Arabidopsis Proteins; D004251:DNA Transposable Elements; D010940:Plant Proteins; C477653:SOS1 protein, Arabidopsis; D017923:Sodium-Hydrogen Exchangers