Nudel and FAK as antagonizing strength modulators of nascent adhesions through paxillin.
Adhesion and detachment are coordinated critical steps during cell migration. Conceptually, efficient migration requires both effective stabilization of membrane protrusions at the leading edge via nascent adhesions and their successful persistence during retraction of the trailing side via disruption of focal adhesions. As nascent adhesions are much smaller in size than focal adhesions, they are expected to exhibit a stronger adhesivity in order to achieve the coordination between cell front and back. Here, we show that Nudel knockdown by interference RNA (RNAi) resulted in cell edge shrinkage due to poor adhesions of membrane protrusions. Nudel bound to paxillin, a scaffold protein of focal contacts, and colocalized with it in areas of active membrane protrusions, presumably at nascent adhesions. The Nudel-paxillin interaction was disrupted by focal adhesion kinase (FAK) in a paxillin-binding-dependent manner. Forced localization of Nudel in all focal contacts by fusing it to paxillin markedly strengthened their adhesivity, whereas overexpression of structurally activated FAK or any paxillin-binding FAK mutant lacking the N-terminal autoinhibitory domain caused cell edge shrinkage. These results suggest a novel mechanism for selective reinforcement of nascent adhesions via interplays of Nudel and FAK with paxillin to facilitate cell migration.
Shan
Yongli
Y
Laboratory of Molecular Cell Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China.
Yu
Lihou
L
Li
Yan
Y
Pan
Youdong
Y
Zhang
Qiangge
Q
Wang
Fubin
F
Chen
Jianfeng
J
Zhu
Xueliang
X
D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D002448:Cell Adhesion; D002460:Cell Line; D002465:Cell Movement; D004847:Epithelial Cells / Q000378:metabolism; D051417:Focal Adhesion Kinase 1 / Q000235:genetics / Q000378:metabolism*; D005786:Gene Expression Regulation; D006367:HeLa Cells; D006801:Humans; D007668:Kidney / Q000166:cytology; D018613:Microscopy, Confocal / Q000379:methods; D009154:Mutation; D051419:Paxillin / Q000235:genetics / Q000378:metabolism*; D034622:RNA Interference; D013997:Time Factors; D001743:Urinary Bladder / Q000166:cytology
D002352:Carrier Proteins; C421108:NDEL1 protein, human; D051419:Paxillin; D051417:Focal Adhesion Kinase 1; C496050:PTK2 protein, human