Atypical protein kinase C activity is required for extracellular matrix degradation and invasion by Src-transformed cells.
Atypical protein kinase C (aPKC) isoforms have been shown to mediate Src-dependent signaling in response to growth factor stimulation. To determine if aPKC activity contributes to the transformed phenotype of cells expressing oncogenic Src, we have examined the activity and function of aPKCs in 3T3 cells expressing viral Src (v-Src). aPKC activity and tyrosine phosphorylation were found to be elevated in some but not all clones of mouse fibroblasts expressing v-Src. aPKC activity was inhibited either by addition of a membrane-permeable pseudosubstrate, by expression of a dominant-negative aPKC, or by RNAi-mediated knockdown of specific aPKC isoforms. aPKC activity contributes to morphological transformation and stress fiber disruption, and is required for migration of Src-transformed cells and for their ability to polarize at the edge of a monolayer. The lambda isoform of aPKC is specifically required for invasion through extracellular matrix in Boyden chamber assays and for degradation of the extracellular matrix in in situ zymography assays. Tyrosine phosphorylation of aPKClambda is required for its ability to promote cell invasion. The defect in invasion upon aPKC inhibition appears to result from a defect in the assembly and/or function of podosomes, invasive adhesions on the ventral surface of the cell that are sites of protease secretion. aPKC was also found to localize to podosomes of v-Src transformed cells, suggesting a direct role for aPKC in podosome assembly and/or function. We conclude that basal or elevated aPKC activity is required for the ability of Src-transformed cells to degrade and invade the extracellular matrix.
Rodriguez
Elena M
EM
Department of Molecular and Cell Biology, University of California at Berkeley, Berkeley, California 94720-3204, USA.
Dunham
Elizabeth E
EE
Martin
G Steven
GS
D000818:Animals; D002461:Cell Line, Transformed; D002465:Cell Movement; D016764:Cell Polarity; D002999:Clone Cells; D004789:Enzyme Activation; D005109:Extracellular Matrix / Q000201:enzymology* / Q000378:metabolism*; D005347:Fibroblasts / Q000166:cytology / Q000201:enzymology; D007527:Isoenzymes / Q000037:antagonists & inhibitors / Q000378:metabolism; D051379:Mice; D015688:Oncogene Protein pp60(v-src) / Q000378:metabolism*; D010766:Phosphorylation; D019000:Phosphotyrosine / Q000378:metabolism; D011493:Protein Kinase C / Q000037:antagonists & inhibitors / Q000378:metabolism*; D000099293:Protein Kinase C-lambda; D000099221:Protein Kinase C zeta
D007527:Isoenzymes; D015688:Oncogene Protein pp60(v-src); D019000:Phosphotyrosine; D011493:Protein Kinase C; D000099293:Protein Kinase C-lambda; D000099221:Protein Kinase C zeta; C115015:PKC-3 protein