Screen of multifunctional monoclonal antibodies against hepatitis B core virus-like particles.
HBc-VLP can be used in an epitope presentation system to carry foreign epitopes and mimic live virus in order to study viral particle uptake, virion-mediated activation and antigen presentation by dendritic cells. In this study, a multifunctional mAb was produced using a novel research strategy. A truncated HBc-VLP bone vector with a special conformation was used as an immunogen and the target hybridoma cell lines were screened by a series of tests; including ELISA, Western blot, and cellular immunofluorescence based on the epitope presentation system. The screened monoclonal antibody was used to identify the HBc-VLP vector, a fusion HBc-VLP vaccine, and intracellular HBV capsids. The new strategy facilitated acquisition of the desired mAbs and will serve as a reference for other VLP-related research.
Sun
Chang
C
Department of Gastroenterology, Changhai Hospital, and Department of Medical Genetics, the Second Military Medical University, Shanghai, China.
Ding
Fei-Xiang
FX
Wang
Fang
F
He
Xiao-Wen
XW
He
Ying
Y
Li
Zhao-Shen
ZS
Sun
Shu-Han
SH
D000818:Animals; D000911:Antibodies, Monoclonal / Q000097:blood / Q000276:immunology*; D000914:Antibodies, Viral / Q000097:blood / Q000276:immunology*; D017951:Antigen Presentation; D002460:Cell Line; D045744:Cell Line, Tumor; D000939:Epitopes / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D005260:Female; D006512:Hepatitis B Core Antigens / Q000235:genetics / Q000276:immunology* / Q000378:metabolism*; D006515:Hepatitis B virus / Q000235:genetics / Q000276:immunology*; D006801:Humans; D007114:Immunization; D008264:Macrophages; D051379:Mice; D008807:Mice, Inbred BALB C; D011487:Protein Conformation; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D014765:Viral Vaccines / Q000276:immunology; D014771:Virion / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D000939:Epitopes; D006512:Hepatitis B Core Antigens; D011994:Recombinant Proteins; D014765:Viral Vaccines