title: CD52 expression in peripheral T-cell lymphomas determined by combined immunophenotyping using tumor cell specific T-cell receptor antibodies.
abstract: For peripheral T-cell lymphomas (PTCL), anti-CD52 (alemtuzumab) therapy is currently under investigation. Previous studies reported widely divergent CD52 expression within PTCL using conventional immunohistochemistry. To accurately discriminate between the presumed mechanistically relevant CD52 expression in tumor versus bystander cells, we employed immunofluorescence double stains using CD52 in combination with an antibody directed against the rearranged T-cell receptor Vbeta-segment of the neoplastic clone in 6 angioimmunoblastic T-cell lymphomas (AITL) and 5 PTCL, unspecified (PTCL-NOS) and, in combination with CD30, in 18 anaplastic large cell lymphomas (ALCL). Tumor cells in all AITL and PTCL-NOS were CD52 positive, while in 17 of 18 ALCL no specific staining was observed. Conversely, the background T- and B-cell infiltrate showed a consistent positivity for CD52. Our approach helps to precisely define CD52 expression in the tumor cell population of PTCL and might therefore be valuable when evaluating the response to alemtuzumab therapy in prospective clinical trials.
authors.affiliation: Institute of Pathology, University of Wuerzburg, Wuerzburg, Germany. geissinger@mail.uni-wuerzburg.de
authors.forename: Eva
authors.initials: E
authors.lastname: Geissinger
authors.forename: Irina
authors.initials: I
authors.lastname: Bonzheim
authors.forename: Sabine
authors.initials: S
authors.lastname: Roth
authors.forename: Andreas
authors.initials: A
authors.lastname: Rosenwald
authors.forename: Hans Konrad
authors.initials: HK
authors.lastname: Müller-Hermelink
authors.forename: Thomas
authors.initials: T
authors.lastname: Rüdiger
mesh_terms: D000906:Antibodies / Q000276:immunology*; D015703:Antigens, CD / Q000032:analysis*; D000951:Antigens, Neoplasm / Q000032:analysis*; D000074301:CD52 Antigen; D005455:Fluorescent Antibody Technique / Q000379:methods; D006023:Glycoproteins / Q000032:analysis*; D006801:Humans; D016130:Immunophenotyping / Q000379:methods*; D017730:Ki-1 Antigen / Q000032:analysis; D017728:Lymphoma, Large-Cell, Anaplastic / Q000276:immunology / Q000473:pathology; D016411:Lymphoma, T-Cell, Peripheral / Q000276:immunology* / Q000473:pathology; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000276:immunology*
chemical_list: D000906:Antibodies; D015703:Antigens, CD; D000951:Antigens, Neoplasm; D000074301:CD52 Antigen; C000613862:CD52 protein, human; D006023:Glycoproteins; D017730:Ki-1 Antigen; D016693:Receptors, Antigen, T-Cell, alpha-beta