title: Distinct phosphatase activity profiles in two strains of Trypanosoma cruzi.
abstract: Phosphorylation of parasite proteins plays a key role in the process of cell invasion by Trypanosoma cruzi, the etiologic agent of Chagas' disease. In this sense, characterization of parasite kinases and phosphatases could open new possibilities for the rational design of chemotherapeutic agents for the treatment of Chagas' disease. In this work, we analyzed phosphatase activities in T. cruzi homogenates from 2 strains belonging to different lineages and with different resistance to oxidative stress. Tulahuen 2 cells (Lineage I) showed higher phosphatase activities and specificity constants when compared to the Y strain (Lineage II). Tulahuen 2 had an optimum phosphatase activity at pH 4.0 and the Y strain at pH 7.0. In both cases, neutral–basic, but not acid, phosphatase activities were increased in the presence of Mg2+. Although calcium had an inhibitory effect at a pH of 7.0 and 8.0 in the Y strain, this inhibition was restricted to pH 8.0 in the other strain. Different substrates and acid phosphotyrosine and alkaline phosphatase inhibitors exhibited distinct effects on the phosphatase activity of both strains. Our results provide a better understanding of T. cruzi phosphatases and reinforce the notion of heterogeneity among T. cruzi populations.
authors.affiliation: Departamento de Bioquímica, IB, UNICAMP, Campinas, SP, Brazil.
authors.forename: R
authors.initials: R
authors.lastname: Morales-Neto
authors.forename: L
authors.initials: L
authors.lastname: Hulshof
authors.forename: C V
authors.initials: CV
authors.lastname: Ferreira
authors.forename: F R
authors.initials: FR
authors.lastname: Gadelha
mesh_terms: D003470:Culture Media; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D009596:Nitrophenols / Q000378:metabolism; D009943:Organophosphorus Compounds / Q000378:metabolism; D010710:Phosphates / Q000032:analysis / Q000378:metabolism; D010744:Phosphoric Monoester Hydrolases / Q000378:metabolism*; D010766:Phosphorylation; D015800:Protozoan Proteins / Q000378:metabolism; D014349:Trypanosoma cruzi / Q000145:classification / Q000201:enzymology* / Q000254:growth & development
chemical_list: D003470:Culture Media; D009596:Nitrophenols; D009943:Organophosphorus Compounds; D010710:Phosphates; D015800:Protozoan Proteins; C008644:nitrophenylphosphate; D010744:Phosphoric Monoester Hydrolases