Interacting with HBsAg compromises resistance of jumping translocation breakpoint protein to ultraviolet radiation-induced apoptosis in 293FT cells.
Jumping translocation breakpoint protein (JTB) is suppressed in many cancers, implying it plays a role in the neoplastic transformation of cells. In order to explore the role of JTB in the carcinogenesis of liver, we used mammalian two-hybrid, co-immunoprecipitation, GST pull-down and laser scanning confocal to verify the interaction between HBs and JTB. According to the results, HBs interacts with JTB. In addition, we further determined that S region within HBs is sufficient for binding JTB. Overexpression of JTB conferred resistance to apoptosis induced by ultraviolet radiation, whereas this effect was compromised by the co-overexpression of HBs.
Pan
Jin-Shui
JS
Division of Gastroenterology, Zhongshan Hospital Xiamen University, Xiamen 361004, Fujian Province, China.
Cai
Jia-Yan
JY
Xie
Chen-Xi
CX
Zhou
Fei
F
Zhang
Zhi-Ping
ZP
Dong
Jing
J
Xu
Hong-Zhi
HZ
Shi
Hua-Xiu
HX
Ren
Jian-Lin
JL
D017209:Apoptosis / Q000502:physiology / Q000528:radiation effects; D015153:Blotting, Western; D002471:Cell Transformation, Neoplastic / Q000235:genetics / Q000378:metabolism* / Q000528:radiation effects; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D006514:Hepatitis B Surface Antigens / Q000378:metabolism*; D019694:Hepatitis B, Chronic / Q000150:complications; D006801:Humans; D047468:Immunoprecipitation; D008113:Liver Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000821:virology; D008565:Membrane Proteins / Q000378:metabolism*; D018613:Microscopy, Confocal; D009363:Neoplasm Proteins / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D011836:Radiation Tolerance / Q000502:physiology*; D014162:Transfection; D020798:Two-Hybrid System Techniques; D014466:Ultraviolet Rays
D006514:Hepatitis B Surface Antigens; C407989:JTB protein, human; D008565:Membrane Proteins; D009363:Neoplasm Proteins