title: Quantification of apolipoprotein A-I mimetic peptide D-4F in rabbit plasma by high-performance liquid chromatography coupled with electrospray ionization tandem mass spectrometry.
abstract: The apolipoprotein A-I mimetic peptide D-4F is a potential therapeutical agent effective in maintaining cardiovascular health. A bioanalytical assay based on high-performance liquid chromatography coupled with electrospray ionization tandem mass spectrometry (LC/MS/MS) to quantitate the D-4F amount in rabbit plasma was developed and validated. A compound with a close structure similarity to the D-4F (only one amino acid A-V altered) was used as an internal standard. Both D-4F and the internal standard were extracted by protein precipitation using acetonitrile/0.2% Triton XL 80N. The correlation coefficient of the calibration curve was 0.9991 in the range 20-40,000 ng/mL. This assay can be used for pharmacokinetic studies of the drug. Also, it may be adjusted for the quantification of other members of apolipoprotein A-I mimetic peptide family.
authors.affiliation: Abbott Laboratories, Santa Clara, CA 95054, USA. xiaoyi.hu@av.abbott.com
authors.forename: Xiaoyi
authors.initials: X
authors.lastname: Hu
authors.forename: Marika
authors.initials: M
authors.lastname: Kamberi
mesh_terms: D000818:Animals; D000924:Anticholesteremic Agents / Q000097:blood* / Q000493:pharmacokinetics; D016632:Apolipoprotein A-I / Q000097:blood* / Q000493:pharmacokinetics; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D011817:Rabbits; D012680:Sensitivity and Specificity; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
chemical_list: D000924:Anticholesteremic Agents; D016632:Apolipoprotein A-I; C465468:D-4F peptide