title: The ubiquitin extension protein S27a is differentially expressed in developing flower organs of Thompson seedless versus Thompson seeded grape isogenic clones.
abstract: In Vitis vinifera L. cv. Thompson Seedless, fertilization occurs but seeds abort, a type of stenospermocarpy. To clone transcripts with differential expression during flower development, suppressive subtractive hybridization was carried out using two isogenic clones 'Thompson seedless' and 'Thompson seeded', at three stages of inflorescence development (from bud break to ~20 days prior to anthesis). Differential screening and sequencing of a forward and reverse subtractive cDNA library yielded several singleton ESTs. One differentially expressed clone in 'Thompson' seeded versus seedless isogenic clones was the ubiquitin extension protein S27a. In situ hybridization demonstrated its significantly higher expression in the carpel and ovaries of 'Thompson' seedless versus seeded isogenic clones during flower development. Overexpression of this gene resulted in abnormal plant regeneration and inhibited shoot development compared to controls; its silencing in embryogenic callus induced cell necrosis and callus death, evidencing tight regulation of this gene in developing organs of grape. S27a overexpression in carpels and integuments of the seedless flower may interfere with normal development of these organs, leading to embryo abortion and seedlessness.
authors.affiliation: Department of Fruit Tree Sciences, Institute of Plant Sciences, Agricultural Research Organization, P.O. Box 6, 50250, Bet Dagan, Israel.
authors.forename: Uri
authors.initials: U
authors.lastname: Hanania
authors.forename: Margarita
authors.initials: M
authors.lastname: Velcheva
authors.forename: Nachman
authors.initials: N
authors.lastname: Sahar
authors.forename: Moshe
authors.initials: M
authors.lastname: Flaishman
authors.forename: Etti
authors.initials: E
authors.lastname: Or
authors.forename: Oded
authors.initials: O
authors.lastname: Degani
authors.forename: Avihai
authors.initials: A
authors.lastname: Perl
mesh_terms: D000595:Amino Acid Sequence; D035264:Flowers / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant; D015723:Gene Library; D020868:Gene Silencing; D017343:Genes, Plant; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D030821:Plants, Genetically Modified / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D018749:RNA, Plant / Q000235:genetics; D012269:Ribosomal Proteins / Q000235:genetics / Q000378:metabolism*; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D025801:Ubiquitin / Q000235:genetics / Q000378:metabolism*; D027843:Vitis / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism
chemical_list: D010940:Plant Proteins; D018749:RNA, Plant; D012269:Ribosomal Proteins; D025801:Ubiquitin; C096227:ribosomal protein S27a