D-amino acid detection in peptides by MALDI-TOF-TOF.
Detection of a D-amino acid residue in natural peptides by mass spectrometry remains a challenging task, as this post-translational modification does not induce any change in molecular mass. To our knowledge, the present article is the first report using matrix-assisted laser desorption/ionization (MALDI) for the discrimination and the quantification of peptide isomers. In this work, we used synthetic hepta- and decapeptides of biological relevance and their isomers. All-L sequences and some isomers containing a D-residue in various positions were analyzed.
Sachon
Emmanuelle
E
Laboratoire des Biomolécules, UMR-CNRS 7206, Université P. et M. Curie, 4 place Jussieu, case courrier 182, 75005 Paris, France.
Clodic
Gilles
G
Galanth
Cécile
C
Amiche
Mohamed
M
Ollivaux
Céline
C
Soyez
Daniel
D
Bolbach
Gérard
G
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis / Q000737:chemistry*; D002352:Carrier Proteins / Q000032:analysis; D007447:Invertebrate Hormones / Q000032:analysis; D007536:Isomerism; D018847:Opioid Peptides / Q000032:analysis; D010455:Peptides / Q000032:analysis* / Q000737:chemistry; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
D000596:Amino Acids; D002352:Carrier Proteins; D007447:Invertebrate Hormones; D018847:Opioid Peptides; D010455:Peptides; C073189:vitellogenesis inhibiting hormone; C030058:dermorphin