Transgene design and delivery into the mouse genome: keys to success.
Mouse transgenesis by pronuclear injection generates random integration events resulting in variable overexpression efficiency. This chapter will outline current strategies for improved transgene design (using plasmids or bacterial artificial chromosomes) and delivery to the mouse genome. The choice of genetic background of embryos for microinjection will also be discussed.
Teboul
Lydia
L
Mary Lyon Centre, Medical Research Council, Harwell, UK. l.teboul@har.mrc.ac.uk
D000818:Animals; D030801:Animals, Genetically Modified; D018244:Chromosomes, Artificial, Yeast; D018014:Gene Transfer Techniques; D005822:Genetic Vectors; D016678:Genome; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D008822:Mice, Transgenic; D008845:Microinjections / Q000379:methods*; D019076:Transgenes / Q000502:physiology*