Genetic biomonitoring of an urban population exposed to mutagenic airborne pollutants.
Biomonitoring studies have increased as a consequence of risks and effects to human health on exposure to environmental contaminants, mainly air pollutants. Genetic biomarkers are useful tools for the early assessment of exposure to occupational and environmental pollution. The objective of the present study was to investigate genotoxic effects on people residing and/or working downwind from an oil refinery in southern Brazil and the mutagenic activity of airborne particulate matter (PM10). Samples of peripheral blood and buccal mucosa cells were evaluated using the single-cell gel electrophoresis assay (comet assay) and the micronucleus (MN) assay, respectively. PM10 samples were collected in the target site and the organic matter extraced with dichloromethane was assessed for mutagenic activity in the Salmonella/microsome assay. The exposed group (n=37) was compared to a reference group (n=37) of subjects living in an urban area with limited traffic and industrial influence, located far from the main industrial areas. All PM10 organic extracts showed mutagenic positive responses and the effect decreased in the presence of S9 mix indicating that the predominant compounds present were direct-acting mutagens. The responses of YGs strains are consistent with aromatic amines and nitroarenes being present in the PM10 extracts. The group in the area under the influence of the oil refinery (exposed group) showed significantly higher DNA damage in lymphocytes than the reference group. The MN frequencies in buccal mucosa were very low for both groups and no difference between groups was observed. No association was found between age and tobacco smoking habit and level of DNA damages measured by the comet assay. The results indicate that the comet assay was a sensitive tool to detect DNA damage in subjects under the influence of an oil refinery, with marked genotoxic activity in the atmospheric environment.
Coronas
Mariana V
MV
Programa de Pesquisas Ambientais, Fundação Estadual de Proteção Ambiental Henrique Luís Roessler (FEPAM), CEP: 90690-000, Porto Alegre, RS, Brazil.
Pereira
Tatiana S
TS
Rocha
Jocelita A V
JA
Lemos
Andréia T
AT
Fachel
Jandyra M G
JM
Salvadori
Daisy M F
DM
Vargas
Vera M F
VM
D000328:Adult; D000393:Air Pollutants / Q000032:analysis* / Q000097:blood / Q000633:toxicity; D004249:DNA Damage; D004781:Environmental Exposure / Q000032:analysis*; D004784:Environmental Monitoring; D019652:Extraction and Processing Industry; D006801:Humans; D019570:Inhalation Exposure / Q000032:analysis*; D009061:Mouth Mucosa / Q000378:metabolism; D009152:Mutagenicity Tests; D009153:Mutagens / Q000032:analysis* / Q000378:metabolism / Q000633:toxicity; D052638:Particulate Matter / Q000032:analysis / Q000097:blood / Q000633:toxicity; D014505:Urban Population / Q000706:statistics & numerical data
D000393:Air Pollutants; D009153:Mutagens; D052638:Particulate Matter