Single-molecule mechanical unfolding of amyloidogenic beta2-microglobulin: the force-spectroscopy approach.
The recombinant production of a novel chimeric polyprotein is described. The new protein contains either wild-type beta(2)-microglobulin (beta(2)m) or its truncated variant (DeltaN6 beta(2)m) (see picture). Structural characterization is achieved by means of single-molecule force spectroscopy studies of specific beta(2)m regions which could be involved in amyloidogenesis.
Sorce
B
B
National Nanotechnology Laboratory of INFM-CNR, Italian Institute of Technology, Via Arnesano 16, 73100 Lecce, Italy.
Sabella
S
S
Sandal
M
M
Samorì
B
B
Santino
A
A
Cingolani
R
R
Rinaldi
R
R
Pompa
P P
PP
D000686:Amyloidosis; D018625:Microscopy, Atomic Force; D015202:Protein Engineering; D017510:Protein Folding; D011994:Recombinant Proteins / Q000737:chemistry; D013050:Spectrometry, Fluorescence; D001613:beta 2-Microglobulin / Q000737:chemistry*
D011994:Recombinant Proteins; D001613:beta 2-Microglobulin