Bacillus solisalsi sp. nov., a halotolerant, alkaliphilic bacterium isolated from soil around a salt lake.
A novel Gram-positive, motile, rod-shaped bacterium isolated from a saline soil in China was characterized by a polyphasic taxonomic approach. The strain, designated YC1(T), was halotolerant [tolerating up to 15 % (w/v) NaCl] and alkaliphilic (growing at a broad pH range of 5-13). 16S rRNA gene sequence analysis revealed that the isolate belonged to the genus Bacillus, showing highest similarity to Bacillus macauensis JCM 13285(T) (98.0 %). However, DNA-DNA hybridization indicated low levels of genomic relatedness with B. macauensis JCM 13285(T) (8.5 %). The major isoprenoid quinone was MK-7 and the cellular fatty acid profile consisted of significant amounts of iso-C(15 : 0) (38.6 %) and anteiso-C(15 : 0) (35.9 %). The predominant polar lipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. The G+C content of the genomic DNA was 41.8 mol%. On the basis of the polyphasic evidence from this study, strain YC1(T) (=KCTC 13181(T)=CGMCC 1.6854(T)) should be classified as the type strain of a novel species of the genus Bacillus, for which the name Bacillus solisalsi sp. nov. is proposed.
Liu
Huan
H
Biotoxin Units of Key Laboratory of Animal Models and Human Disease Mechanisms, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, Yunnan, PR China.
Zhou
Yu
Y
Liu
Rui
R
Zhang
Ke-Yun
KY
Lai
Ren
R
D001407:Bacillus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology; D015373:Bacterial Typing Techniques; D001482:Base Composition; D002681:China; D004269:DNA, Bacterial / Q000032:analysis; D004275:DNA, Ribosomal / Q000032:analysis; D005227:Fatty Acids / Q000032:analysis; D005618:Fresh Water / Q000382:microbiology*; D006863:Hydrogen-Ion Concentration; D008055:Lipids / Q000032:analysis; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010641:Phenotype; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012965:Sodium Chloride / Q000378:metabolism; D012988:Soil Microbiology; D013045:Species Specificity
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D008055:Lipids; D012336:RNA, Ribosomal, 16S; D012965:Sodium Chloride