Piceatannol, a natural stilbene from grapes, induces G1 cell cycle arrest in androgen-insensitive DU145 human prostate cancer cells via the inhibition of CDK activity.
We have examined whether and by what mechanism piceatannol inhibits cell cycle progression in DU145 cells. The treatment of cells with piceatannol for 24h resulted in an increase in the percentage of cells in G1 phase and dose-dependent decreases in [(3)H]thymidine incorporation, as well as in protein levels of cyclin A, cyclin D1, and cyclin-dependent kinase (CDK)2 and CDK4. Piceatannol exerted no effect on the levels of p21(WAF1/CIP1) or p27(KIP1). Piceatannol reduced CDK4 and CDK2 activity. These results indicate that delaying G1 cell cycle progression contributes to the piceatannol-mediated inhibition of DU145 cell growth, which may be mediated via the inhibition of CDK activity.
Lee
Yeo Myeong
YM
Department of Food Science and Nutrition, Hallym University, Chuncheon 200-702, Republic of Korea.
Lim
Do Young
DY
Cho
Han Jin
HJ
Seon
Mi Ra
MR
Kim
Jin-Kyung
JK
Lee
Boo-Yong
BY
Park
Jung Han Yoon
JH
D000970:Antineoplastic Agents / Q000494:pharmacology*; D002453:Cell Cycle / Q000187:drug effects*; D045744:Cell Line, Tumor; D018844:Cyclin-Dependent Kinases / Q000187:drug effects* / Q000378:metabolism; D005434:Flow Cytometry; D006801:Humans; D015151:Immunoblotting; D047468:Immunoprecipitation; D008297:Male; D011471:Prostatic Neoplasms / Q000378:metabolism*; D013267:Stilbenes / Q000494:pharmacology*
D000970:Antineoplastic Agents; D013267:Stilbenes; C041525:3,3',4,5'-tetrahydroxystilbene; D018844:Cyclin-Dependent Kinases