title: Histoplanimetrical study on the spatial relationship of distribution of indigenous bacteria with mucosal lymphatic follicles in alimentary tract of rat.
abstract: The spatial relationship between the distribution of indigenous bacteria (IB) and the situation of mucosal lymphatic follicles (LF) is histoplanimetrically studied in the rat alimentary tract. From the oral cavity to the nonglandular part of the stomach, IB adhered to the corneal layer of the most luminal mucosa. In the glandular part of the stomach, IB adhered only to the most luminal mucosa but not in the gastric pits. In the small intestine, IB consistently adhered around the apices of both intestinal villi and the domes, and their amounts decreased toward their basal portions. No IB entered the intestinal crypts. In the large intestine, IB consistently adhered to the most luminal mucosa. Numerous IB were suspended in the intestinal crypts of both the cecum and the proximal colon, whereas there were no IB in the crypts of the distal colon and the rectum. When IB spread over the basal portions of the intestinal villi, IB with the same morphology were detected on the neighboring LF, whereas no bacteria were detected on the neighboring LF, when IB were located in the apical to middle portions of the intestinal villi. This close relationship between the distribution of IB and mucosal LF was also observed in the large intestine. These results suggest that the most luminal mucosae are a fundamental settlement site of IB throughout the alimentary tract and that the hyperproliferation of IB's colonies might be detected by neighboring LF in the rat intestine.
authors.affiliation: Department of Bioresource and Agrobiosciences, Kobe University, Kobe, Japan.
authors.forename: Kenkichi
authors.initials: K
authors.lastname: Yamamoto
authors.forename: Wang-Mei
authors.initials: WM
authors.lastname: Qi
authors.forename: Yuh
authors.initials: Y
authors.lastname: Yokoo
authors.forename: Hidenori
authors.initials: H
authors.lastname: Miyata
authors.forename: Kankanam Gamage Sanath
authors.initials: KG
authors.lastname: Udayanga
authors.forename: Junichi
authors.initials: J
authors.lastname: Kawano
authors.forename: Toshifumi
authors.initials: T
authors.lastname: Yokoyama
authors.forename: Nobuhiko
authors.initials: N
authors.lastname: Hoshi
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Kitagawa
mesh_terms: D000818:Animals; D015169:Colony Count, Microbial; D004064:Digestive System / Q000033:anatomy & histology / Q000382:microbiology*; D005753:Gastric Mucosa / Q000033:anatomy & histology / Q000382:microbiology*; D007413:Intestinal Mucosa / Q000033:anatomy & histology / Q000382:microbiology*; D008221:Lymphoid Tissue / Q000033:anatomy & histology / Q000382:microbiology*; D008297:Male; D051381:Rats; D017208:Rats, Wistar