title: Sediment genotoxicity in the Tietê River (São Paulo, Brazil): in vitro comet assay versus in situ micronucleus assay studies.
abstract: The in vitro comet assay with the permanent fish cell line RTL-W1 and the in situ micronucleus assay using erythrocytes from indigenous tilapia (Oreochromis niloticus) were used to detect genotoxicity in Tietê River sediments (São Paulo, Brazil). Either test was successful in identifying site-specific differences in genotoxicity, with a high correlation between in situ and in vitro results indicating the relevance of the latter even for environmental studies. Discharges from São Paulo city have major impact on genotoxic effects by sediment-bound contaminants; however, overall genotoxicity decreases downstream. The high genotoxic burden of the Tietê River warrants measures to reduce the input of toxic effluents.
authors.affiliation: Aquatic Ecology and Toxicology Section, Heidelberg Institute of Zoology, University of Heidelberg, Im Neuenheimer Feld 230, 69120 Heidelberg, Germany. rocha@zoo.uni-heidelberg.de
authors.forename: Paula Suares
authors.initials: PS
authors.lastname: Rocha
authors.forename: George Luiz
authors.initials: GL
authors.lastname: Luvizotto
authors.forename: Thomas
authors.initials: T
authors.lastname: Kosmehl
authors.forename: Melanie
authors.initials: M
authors.lastname: Böttcher
authors.forename: Volker
authors.initials: V
authors.lastname: Storch
authors.forename: Thomas
authors.initials: T
authors.lastname: Braunbeck
authors.forename: Henner
authors.initials: H
authors.lastname: Hollert
mesh_terms: D000818:Animals; D001938:Brazil; D002460:Cell Line; D020552:Comet Assay; D004249:DNA Damage; D004784:Environmental Monitoring / Q000379:methods*; D004912:Erythrocytes / Q000187:drug effects; D005347:Fibroblasts / Q000187:drug effects; D019015:Geologic Sediments / Q000737:chemistry*; D008099:Liver / Q000166:cytology; D048629:Micronuclei, Chromosome-Defective / Q000139:chemically induced*; D015162:Micronucleus Tests; D017686:Oncorhynchus mykiss; D045483:Rivers / Q000737:chemistry*; D017210:Tilapia / Q000097:blood; D014874:Water Pollutants, Chemical / Q000633:toxicity*
chemical_list: D014874:Water Pollutants, Chemical