title: High-performance liquid chromatographic purification of oligomeric procyanidins, trimers up to nonamers, derived from the bark of Jatoba (Hymenaea courbaril).
abstract: Procyanidin oligomers with different degrees of polymerization (up to nonamers) were efficiently purified from the bark of Jatoba (Hymenaea courbaril) by using a recently developed chromatographic separation method. Purification relied on a hydrogen bonding interaction between phenolic hydroxyl groups of the procyanidins and polyethylene glycol (PEG)-coated resin in a packed column. The individual procyanidins were identified by using electrospray ionization mass spectrometry (ESI-MS) and verified by a thiolytic degradation analysis. Our results demonstrate that Jatoba bark contained a large amount of procyanidins from monomer to nonamers or higher polymers composed of only B-type linked units (flavan-3-ol units linked through C-4 to C-8 (or C-6)) of epicatechin (EC) without gallate esters.
authors.affiliation: Central Laboratories for Frontier Technology, Kirin Holdings Co., Ltd., Yokohama, Japan.
authors.forename: Katsunori
authors.initials: K
authors.lastname: Sasaki
authors.forename: Yasuko
authors.initials: Y
authors.lastname: Matsukura
authors.forename: Kumiko
authors.initials: K
authors.lastname: Shijima
authors.forename: Mika
authors.initials: M
authors.lastname: Miyake
authors.forename: Daisuke
authors.initials: D
authors.lastname: Fujiwara
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Konishi
mesh_terms: D001704:Biopolymers / Q000737:chemistry / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D052597:Hymenaea / Q000737:chemistry*; D024301:Plant Bark / Q000737:chemistry*; D044945:Proanthocyanidins / Q000737:chemistry / Q000302:isolation & purification*; D021241:Spectrometry, Mass, Electrospray Ionization; D016339:Spectrometry, Mass, Fast Atom Bombardment
chemical_list: D001704:Biopolymers; D044945:Proanthocyanidins