title: Phenotypic and genetic diversity of rice seed-associated bacteria and their role in pathogenicity and biological control.
abstract: OBJECTIVE
  To study the phenotypic and genetic diversity of culturable bacteria associated with rice seed and to asses the antagonistic and pathogenic potential of the isolated bacteria.
  
  
  RESULTS
  Seed of rice cultivar PSBRc14 was collected from farmers' fields of irrigated lowland in southern Luzon, Philippines. Isolations of distinct colonies yielded 498 isolates. Classification of the isolates according to similarities in cellular characteristics, whole-cell fatty acid composition, and colony appearance differentiated 101 morphotype groups. Predominant bacteria were Coryneform spp., Pantoea spp. and Pseudomonas spp. Other bacteria regularly present were Actinomycetes spp., Bacillus pumilus, B. subtilis, Burkholderia glumae, Enterobacter cloacae, Paenibacillus polymyxa, Staphylococcus spp. and Xanthomonas spp. The genetic diversity among isolates was assessed by BOX-PCR fingerprinting of genomic DNA and represented 284 fingerprint types (FPTs). Most FPTs (78%) were not shared among samples, while eight FPTs occurred frequently in the samples. Seven of these FPTs also occurred frequently in a previous collection made from rainfed lowlands of Iloilo island, Philippines. Sixteen per cent of the isolates inhibited in vitro the mycelial growth of the rice pathogens Rhizoctonia solani and Pyricularia grisea, whereas 4% were pathogens identified as Burkholderia glumae, Burkholderia gladioli and Acidovorax avenae ssp. avenae.
  
  
  CONCLUSIONS
  This study reveals a broad morphological and genetic diversity of bacteria present on seed of a single rice cultivar.
  
  
  CONCLUSIONS
  This line of work contributes to a better understanding of the microbial diversity present on rice seed and stresses its importance as a carrier of antagonists and pathogens.
authors.affiliation: Entomology and Plant Pathology Division, International Rice Research Institute (IRRI), Metro Manila, Philippines.
authors.forename: B
authors.initials: B
authors.lastname: Cottyn
authors.forename: J
authors.initials: J
authors.lastname: Debode
authors.forename: E
authors.initials: E
authors.lastname: Regalado
authors.forename: T W
authors.initials: TW
authors.lastname: Mew
authors.forename: J
authors.initials: J
authors.lastname: Swings
mesh_terms: D000898:Antibiosis; D015169:Colony Count, Microbial; D005658:Fungi / Q000254:growth & development*; D014644:Genetic Variation; D006090:Gram-Negative Bacteria / Q000145:classification / Q000254:growth & development / Q000302:isolation & purification* / Q000472:pathogenicity*; D006094:Gram-Positive Bacteria / Q000145:classification / Q000254:growth & development / Q000302:isolation & purification* / Q000472:pathogenicity*; D025282:Mycelium / Q000254:growth & development; D012275:Oryza / Q000382:microbiology*; D010572:Pest Control, Biological; D010641:Phenotype; D012639:Seeds / Q000382:microbiology*