Synthesis and evaluation of an acyl-chain unsaturated analog of the Th2 biasing, immunostimulatory glycolipid, OCH.
An alpha-galactosyl ceramide (alpha-GalCer) 2 was synthesized and evaluated for its ability to stimulate iNKT-cell proliferation and elicit T-helper cytokines, IL-4 and IFNgamma. Compound 2 combines the acyl chain of the potent, Th2 biasing alpha-GalCer 1 with a sphingoid base of the same length as that found in OCH, which also exhibits Th2 skewing, Such complementation may enhance cytokine bias, which is thought to be important for therapeutic applications of iNKT cell stimulation. Two related alpha-GalCers, 3 and 4, with saturated acyl chains were prepared for comparison.
Velmourougane
Geetha
G
Department of Chemistry, University of Connecticut, Storrs, CT 06269-3060, United States.
Raju
Ravinder
R
Bricard
Gabriel
G
Im
Jin S
JS
Besra
Gurdyal S
GS
Porcelli
Steven A
SA
Howell
Amy R
AR
D000276:Adjuvants, Immunologic / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology; D000473:Alkanes / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology; D000818:Animals; D055610:Antigens, CD1d / Q000276:immunology / Q000378:metabolism; D005699:Galactosylceramides / Q000737:chemistry*; D007371:Interferon-gamma / Q000378:metabolism; D015847:Interleukin-4 / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D009005:Monosaccharides / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology; D055611:Natural Killer T-Cells / Q000187:drug effects / Q000276:immunology; D018418:Th2 Cells / Q000187:drug effects / Q000276:immunology*
D000276:Adjuvants, Immunologic; D000473:Alkanes; D055610:Antigens, CD1d; D005699:Galactosylceramides; D009005:Monosaccharides; C542851:OCH compound; C493154:alpha-galactosylceramide; D015847:Interleukin-4; D007371:Interferon-gamma