[Protective effect of blue light-absorbing IOLs on the human retinal pigment epithelium].
METHODS
Primary human RPE cells were exposed to white light and either a SN60AT or SA60AT IOL was placed in the light beam. After 15-60 min of irradiation, viability, induction of apoptosis and cell death were determined in primary human RPE cells. Expression of vascular endothelial growth factor A (VEGF-A) and the anti-apoptotic XIAP protein and their mRNA were determined by RT-PCR, Western blot analysis and ELISA.


RESULTS
Light exposure decreased cell viability depending on the duration of irradiation. Light-induced cell death and apoptosis as well as decrease of XIAP expression and cellular viability were significantly reduced by both the SN60AT and SA60AT IOL. In addition, these protective effects regarding light-induced cell damage were significantly stronger in the presence of the blue light-filtering SN60AT IOL compared to the SA60AT IOL.


CONCLUSIONS
Both UV-filtering and blue light-absorbing IOLs reduce light-induced RPE damage. The blue light-absorbing IOL further reduced damage compared to the conventional IOL, which supports the hypothesis of possibly also preventing retinal damage in clinical use.
Kernt
M
M
Augenklinik der Ludwig-Maximilians-Universität, Mathildenstrasse 8, 80336, München, Deutschland. Marcus.Kernt@med.uni-muenchen.de
Hirneiss
C
C
Neubauer
A S
AS
Lackerbauer
C A
CA
Eibl
K H
KH
Wolf
A
A
Ulbig
Mw
M
Kampik
A
A
D017209:Apoptosis / Q000528:radiation effects; D002470:Cell Survival / Q000528:radiation effects; D004797:Enzyme-Linked Immunosorbent Assay; D005374:Filtration / Q000295:instrumentation*; D005786:Gene Expression Regulation / Q000528:radiation effects; D006801:Humans; D066298:In Vitro Techniques; D007910:Lenses, Intraocular; D008027:Light / Q000009:adverse effects*; D008268:Macular Degeneration / Q000209:etiology / Q000517:prevention & control; D010857:Pigment Epithelium of Eye / Q000528:radiation effects*; D011474:Prosthesis Design; D012333:RNA, Messenger / Q000235:genetics; D011835:Radiation Protection / Q000295:instrumentation*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014466:Ultraviolet Rays / Q000009:adverse effects*; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics; D051636:X-Linked Inhibitor of Apoptosis Protein / Q000235:genetics
D012333:RNA, Messenger; C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A; D051636:X-Linked Inhibitor of Apoptosis Protein; C496866:XIAP protein, human