title: Direct protein detection with a nano-interdigitated array gate MOSFET.
abstract: A new protein sensor is demonstrated by replacing the gate of a metal oxide semiconductor field effect transistor (MOSFET) with a nano-interdigitated array (nIDA). The sensor is able to detect the binding reaction of a typical antibody Ixodes ricinus immunosuppressor (anti-Iris) protein at a concentration lower than 1 ng/ml. The sensor exhibits a high selectivity and reproducible specific detection. We provide a simple model that describes the behavior of the sensor and explains the origin of its high sensitivity. The simulated and experimental results indicate that the drain current of nIDA-gate MOSFET sensor is significantly increased with the successive binding of the thiol layer, Iris and anti-Iris protein layers. It is found that the sensor detection limit can be improved by well optimizing the geometrical parameters of nIDA-gate MOSFET. This nanobiosensor, with real-time and label-free capabilities, can easily be used for the detection of other proteins, DNA, virus and cancer markers. Moreover, an on-chip associated electronics nearby the sensor can be integrated since its fabrication is compatible with complementary metal oxide semiconductor (CMOS) technology.
authors.affiliation: Microelectronics Laboratory (DICE) and CERMIN, Université catholique de Louvain (UCL), Place du Levant 3, 1348 Louvain-la-Neuve, Belgium. Xiaohui.tang@uclouvain.be
authors.forename: Xiaohui
authors.initials: X
authors.lastname: Tang
authors.forename: Alain M
authors.initials: AM
authors.lastname: Jonas
authors.forename: Bernard
authors.initials: B
authors.lastname: Nysten
authors.forename: Sophie
authors.initials: S
authors.lastname: Demoustier-Champagne
authors.forename: Franoise
authors.initials: F
authors.lastname: Blondeau
authors.forename: Pierre-Paul
authors.initials: PP
authors.lastname: Prévot
authors.forename: Rémi
authors.initials: R
authors.lastname: Pampin
authors.forename: Edmond
authors.initials: E
authors.lastname: Godfroid
authors.forename: Benjamin
authors.initials: B
authors.lastname: Iñiguez
authors.forename: Jean-Pierre
authors.initials: JP
authors.lastname: Colinge
authors.forename: Jean-Pierre
authors.initials: JP
authors.lastname: Raskin
authors.forename: Denis
authors.initials: D
authors.lastname: Flandre
authors.forename: Vincent
authors.initials: V
authors.lastname: Bayot
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation*; D017076:Computer-Aided Design; D004563:Electrochemistry / Q000295:instrumentation*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D036103:Nanotechnology / Q000295:instrumentation*; D040081:Protein Array Analysis / Q000295:instrumentation*; D025941:Protein Interaction Mapping / Q000295:instrumentation*; D012815:Signal Processing, Computer-Assisted / Q000295:instrumentation*; D014159:Transducers; D014173:Transistors, Electronic