title: Effects of the domestic use of a disclosing solution on the denture biofilm: a preliminary study.
abstract: To investigate the effect of the home use of a disclosing agent on the microbial composition of denture biofilm, by means of a cross-over randomized clinical trial. Two interventions were tested during 7 days each: (I) oral and denture hygiene instructions and (II) instructions associated with the home use of a disclosing agent (1% neutral red). Eleven participants with visible biofilm deposits over their maxillary complete dentures were randomly assigned to one of the two sequences of interventions: (i) I followed by II, and (ii) II followed by I. A washout period of 7 days was established. After each intervention, samples of denture biofilm were evaluated by DNA checkerboard hybridization for the detection of Candida spp. and 17 bacterial species. Counts were low for all the tested species, and no significant difference was found between the tested interventions (Wilcoxon test, P > 0.05). The home use of a disclosing agent does not remarkably change the composition of denture biofilm.
authors.affiliation: Department of Dental Materials and Prosthodontics, Ribeirão Preto School of Dentistry, University of São Paulo, Ribeirão Preto, Brazil. raphael@forp.usp.br
authors.forename: R F
authors.initials: RF
authors.lastname: De Souza
authors.forename: C
authors.initials: C
authors.lastname: Nascimento
authors.forename: R R
authors.initials: RR
authors.lastname: Regis
authors.forename: C H
authors.initials: CH
authors.lastname: Silva-Lovato
authors.forename: H F O
authors.initials: HF
authors.lastname: Paranhos
mesh_terms: D000368:Aged; D018441:Biofilms / Q000254:growth & development*; D002175:Candida / Q000502:physiology; D004396:Coloring Agents; D018592:Cross-Over Studies; D003824:Denture, Complete / Q000382:microbiology*; D004926:Escherichia coli / Q000502:physiology; D005260:Female; D006801:Humans; D008297:Male; D009910:Oral Hygiene / Q000592:standards*; D009911:Oral Hygiene Index; D011550:Pseudomonas aeruginosa / Q000502:physiology; D013210:Staphylococcus / Q000502:physiology; D014099:Toothbrushing / Q000379:methods
chemical_list: D004396:Coloring Agents