[Influence of DNAzymes against cyclin D1 in tumor cell cycle].
In this study, DNAzymes against cyclin D1 (cyclin D1-DRz) were designed according to the secondary structure of cyclin D1 mRNA which was computed with RNAdraw and Mfold. Cyclin D1-DRz were transfected into tumor cell line u251 and HeLa by oligofectamine. The expression of cyclin D1 was detected by RT-PCR. It was shown that the expression of cyclin D1 gene was suppressed obviously, and the expressions of other cell-cycle related genes such as cyclin E1, cyclin A1 and cyclin B1 were also declined. The cell cycle analysis of tumor cells tansfected with cyclin D1-DRz revealed an arrestment in the G0/G1 phase. In conclusion, the approach is effective and feasible for designing DNAzyme. Cyclin D1-DRz is useful for interfering with the cell cycle procession of tumor cells.
Li
Dan
D
Institute of Life Science and Biotechnology, Hunan University, Changsha 410082, China.
Zhu
Junyi
J
Zhou
Yayan
Y
Liu
Xuanming
X
D001483:Base Sequence; D002453:Cell Cycle / Q000187:drug effects*; D019938:Cyclin D1 / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000378:metabolism; D021881:DNA, Catalytic / Q000235:genetics / Q000378:metabolism* / Q000494:pharmacology*; D006367:HeLa Cells; D006801:Humans; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D014162:Transfection
D021881:DNA, Catalytic; D012333:RNA, Messenger; D019938:Cyclin D1