Transformation and apoptosis of NIH/3T3 cells treated with nickel-smelting fumes.
The purpose of this study was to investigate the transformation and apoptosis of NIH/3T3 cells treated with nickel (Ni) smelting fumes. Cytotoxicity of NIH/3T3 cells was detected with a methyl thiazolyl tetrazolium (MTT) colorimetric assay. The cell translation model was established by cell focus translation using two types of Ni-smelting fumes from a Ni smelting plant in China. The transformed focus was determined by soft agar culture assay. The apoptotic characteristics of NIH/3T3 cells treated with Ni-smelting fumes were detected by flow cytometry using Annexin V-FITC and PI as markers. The DNA fragment of apoptosis in NIH/3T3 cells treated with nickel smelting fumes was detected by observing agarose electrophoresis and morphological characteristics of cells under electron microscopy. With increase in exposure time, growth of NIH/3T3 cells was inhibited. The NIH/3T3 cell transformation model was established successfully using two Ni-smelting fumes, and the transformed cells grow in soft agar. No apoptosis peak was detected by flow cytometry. Apoptotic cells characterized by necrosis were observed using electron microscopy. There was no apparent "ladder" observed by DNA fragment analysis. Data indicated that Ni-smelting fumes produced cytotoxicity by mechanisms associated with necrosis but not apoptosis.
Jin
Yan-Tao
YT
Department of Occupational Health, Public Health College, Harbin Medical University, Heilongjiang Province, People's Republic of China.
Wu
Yong-Hui
YH
Hu
Fu-Lan
FL
Hu
Xue-Ying
XY
D000393:Air Pollutants / Q000633:toxicity*; D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects*; D007928:Lethal Dose 50; D008669:Metallurgy; D051379:Mice; D041681:NIH 3T3 Cells; D009336:Necrosis / Q000473:pathology; D009532:Nickel
D000393:Air Pollutants; D009532:Nickel