title: Abused inhalants enhance GABA-mediated synaptic inhibition.
abstract: Abused inhalants are widely used, especially among school-age children and teenagers, and are 'gateway' drugs leading to the abuse of alcohol and other addictive substances. In spite of this widespread use, little is known about the effects produced by inhalants on the central nervous system. The similarity in behavioral effects produced by inhalants and inhaled anesthetics, together with their common chemical features, prompted this study of inhalant actions on a well-characterized anesthetic target, GABA synapses. Whole-cell patch clamp recordings were conducted on CA1 pyramidal neurons in rat hippocampal brain slices to measure effects on resting membrane properties, action potential discharge, and GABA-mediated inhibitory responses. Toluene, 1,1,1-trichloroethane, and trichloroethylene depressed CA1 excitability in a concentration-dependent and reversible manner. This depression appeared to involve enhanced GABA-mediated inhibition, evident in its reversal by a GABA receptor antagonist. Consistent with this, the abused inhalants increased inhibitory postsynaptic potentials produced using minimal stimulation of stratum radiatum inputs to CA1 neurons, in the presence of CNQX and APV to block excitatory synaptic responses and GGP to block GABA(B) responses. The enhanced inhibition appeared to come about by a presynaptic action on GABA nerve terminals, because spontaneous inhibitory postsynaptic current (IPSC) frequency was increased with no change in the amplitude of postsynaptic currents, both in the presence and absence of tetrodotoxin used to block interneuron action potentials and cadmium used to block calcium influx into nerve terminals. The toluene-induced increase in mIPSC frequency was blocked by dantrolene or ryanodine, indicating that the abused inhalant acted to increase the release of calcium from intracellular nerve terminal stores. This presynaptic action produced by abused inhalants is shared by inhaled anesthetics and would contribute to the altered behavioral effects produced by both classes of drugs, and could be especially important in the context of a disruption of learning and memory by abused inhalants.
authors.affiliation: Department of Anesthesia, Stanford University School of Medicine, Stanford, CA 94305-5117, USA. maciver@stanford.edu
authors.forename: M Bruce
authors.initials: MB
authors.lastname: MacIver
mesh_terms: D000200:Action Potentials / Q000187:drug effects; D000818:Animals; D000831:Animals, Newborn; D001703:Biophysics; D002118:Calcium / Q000378:metabolism; D003620:Dantrolene / Q000494:pharmacology; D004347:Drug Interactions / Q000502:physiology; D004558:Electric Stimulation / Q000379:methods; D006624:Hippocampus / Q000166:cytology*; D066298:In Vitro Techniques; D053444:Inhibitory Postsynaptic Potentials / Q000187:drug effects* / Q000502:physiology; D009125:Muscle Relaxants, Central / Q000494:pharmacology; D009433:Neural Inhibition / Q000187:drug effects* / Q000502:physiology; D018408:Patch-Clamp Techniques / Q000379:methods; D017966:Pyramidal Cells / Q000187:drug effects*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012433:Ryanodine / Q000494:pharmacology; D026941:Sodium Channel Blockers / Q000494:pharmacology; D012997:Solvents / Q000494:pharmacology; D013569:Synapses / Q000187:drug effects*; D013779:Tetrodotoxin / Q000494:pharmacology; D014050:Toluene / Q000494:pharmacology; D014240:Trichloroethanes; D014241:Trichloroethylene; D005680:gamma-Aminobutyric Acid / Q000494:pharmacology*
chemical_list: D009125:Muscle Relaxants, Central; D026941:Sodium Channel Blockers; D012997:Solvents; D014240:Trichloroethanes; C024566:1,1,1-trichloroethane; D012433:Ryanodine; D014241:Trichloroethylene; D014050:Toluene; D013779:Tetrodotoxin; D005680:gamma-Aminobutyric Acid; D003620:Dantrolene; D002118:Calcium