Interleukin-12 gene polymorphism and cervical cancer risk.
OBJECTIVE
: To investigate the effect of interleukin-12 p40 gene (IL-12β) 3'-untranslated region polymorphism on the risk of cervical cancer.


METHODS
DNA was isolated from peripheral blood of 200 patients with cervical cancer and 200 healthy controls. Polymerase chain reaction restriction fragment length polymorphism was used for the detection of IL-12β (1188A/C) gene polymorphism.


RESULTS
It was observed that genotypes AC [odds ratio (OR) = 1.80, 95% confidence interval (CI) = 1.17-2.78] and AC + CC (OR = 1.71, 95% CI = 1.11-2.63, P = 0.01) increase the risk of cervical cancer. The strength of the risk in passive smokers with AC and AC + CC genotypes was increased to more than 2.8 times in comparison with a nonsmoker with AA genotype (OR = 2.94, 95% CI = 1.56-5.55, P < 0.001; OR = 2.83, 95% CI = 1.52-5.31, P < 0.001, respectively).


CONCLUSIONS
This is the first study to provide an evidence for the association of IL-12β (1188A/C) gene polymorphism with the risk of cervical cancer.
Tamandani
Dor Mohammad Kordi
DM
Department of Biotechnology, Panjab University, Chandigarh, India. dor_kordi@yahoo.com
Shekari
Mohammad
M
Suri
V
V
D000328:Adult; D016022:Case-Control Studies; D005260:Female; D020022:Genetic Predisposition to Disease; D006801:Humans; D053762:Interleukin-12 Subunit p40 / Q000235:genetics*; D008423:Maternal Age; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D012306:Risk; D002583:Uterine Cervical Neoplasms / Q000235:genetics*
D053762:Interleukin-12 Subunit p40