title: Lettuce-induced anaphylaxis. Identification of the allergen involved.
abstract: BACKGROUND
  Only 2 allergenic proteins have been described in lettuce allergy: a 16-kDa protein (putative profilin) and a lipid transfer protein (LTP) named Lac s 1.
  
  
  OBJECTIVE
  Our aim was to identify the allergens involved in the anaphylactic reactions of 2 patients who had eaten lettuce.
  
  
  METHODS
  The study was performed by Ig (immunoglobulin)-E immunodetection and immunodetection-inhibition assays.
  
  
  RESULTS
  Both patients' sera showed specific IgE binding to a single protein from the crude lettuce extract (apparent molecular weight of 14 kDa). To characterize the allergen detected, the lettuce extract underwent proteolytic digestion and heat treatment and was highly resistant to both. The patients' sera also recognized the major peach allergen Pru p 3 by immunodetection. When the lettuce allergen was incubated with both Pru p 3 from peach peel and recombinant Pru p 3, the immunodetection-inhibition assay indicated that patients were sensitized to the lettuce LTP Lac s 1.
  
  
  CONCLUSIONS
  The allergen involved in the lettuce-induced anaphylaxis of our patients was the LTP Lac s 1.
authors.affiliation: Department of Allergology, Hospital General Yagüe, Burgos, Spain.
authors.forename: O
authors.initials: O
authors.lastname: Bascones
authors.forename: R
authors.initials: R
authors.lastname: Rodríguez-Pérez
authors.forename: S
authors.initials: S
authors.lastname: Juste
authors.forename: I
authors.initials: I
authors.lastname: Moneo
authors.forename: M L
authors.initials: ML
authors.lastname: Caballero
mesh_terms: D000328:Adult; D000485:Allergens / Q000276:immunology / Q000378:metabolism; D000707:Anaphylaxis / Q000209:etiology / Q000276:immunology*; D000799:Angioedema; D052179:Antigens, Plant / Q000276:immunology* / Q000378:metabolism; D001986:Bronchial Spasm; D005260:Female; D005512:Food Hypersensitivity / Q000097:blood / Q000150:complications / Q000276:immunology* / Q000503:physiopathology; D006358:Hot Temperature; D006801:Humans; D007073:Immunoglobulin E / Q000097:blood / Q000276:immunology; D018545:Lactuca / Q000009:adverse effects*; D027845:Malus / Q000009:adverse effects; D018716:Molecular Mimicry; D010447:Peptide Hydrolases / Q000378:metabolism; D010940:Plant Proteins; D027861:Prunus / Q000009:adverse effects; D017386:Sequence Homology, Amino Acid; D012882:Skin Tests; D014581:Urticaria
chemical_list: D000485:Allergens; D052179:Antigens, Plant; C521002:Lac s 1 protein, Lactuca sativa; D010940:Plant Proteins; C463181:Pru p 3 allergen; D007073:Immunoglobulin E; D010447:Peptide Hydrolases