Basophil activation tests in the evaluation of immediate drug hypersensitivity.
OBJECTIVE
The aim of this study was to confirm the applicability of the basophil activation test (BAT) in the in-vitro diagnosis of drug allergy reactions.


RESULTS
The results obtained in terms of sensitivity and specificity with BAT are encouraging and in significant number of cases can establish the diagnosis.


CONCLUSIONS
BAT sensitivity in beta-lactam allergy was 50%, and specificity ranged from 89 to 97%. There are several studies to validate the BAT in allergy to muscle relaxants showing a sensitivity ranging from 54 to 64% with a specificity of 100 and 93%. The sensitivity of a test for evaluating immediate allergic reactions to drugs may decrease over time. To date, the BAT is the only in-vitro diagnostic method that has been validated for the diagnosis of both IgE-mediated and hypersensitivity reactions to NSAIDs. With respect to other drugs, they are nonetheless interesting as they include the evaluation of allergy to drugs that cannot be studied by other in-vitro techniques. All these data suggest that although a full validation of the test is required, BAT is a potential diagnostic method for evaluating immediate allergic reactions to drugs and NSAID hypersensitivity reactions.
Sanz
María L
ML
Department of Allergology and Clinical Immunology, University Clinic of Navarra, School of Medicine, University of Navarra, Apartado 4209, Pamplona 31080, Spain.mlsanzlar@unav.es
Gamboa
Pedro M
PM
Mayorga
Cristobalina
C
D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal; D015549:Basophil Degranulation Test / Q000379:methods*; D004342:Drug Hypersensitivity / Q000175:diagnosis* / Q000276:immunology; D000939:Epitopes; D005240:Feasibility Studies; D006801:Humans; D006969:Hypersensitivity, Immediate / Q000175:diagnosis* / Q000276:immunology; D011237:Predictive Value of Tests; D012680:Sensitivity and Specificity; D013997:Time Factors; D047090:beta-Lactams / Q000008:administration & dosage / Q000009:adverse effects* / Q000737:chemistry
D000894:Anti-Inflammatory Agents, Non-Steroidal; D000939:Epitopes; D047090:beta-Lactams