Comparative study of hop-containing products on human cytochrome p450-mediated metabolism.
Thirty-five national and international brands of beer were examined for their potential to affect human cytochrome P450 (CYP)-mediated metabolism. They represented the two main categories of beer, ales and lagers, and included a number of specialty products including bitter (porter, stout), coffee, ice, wheat, Pilsner, and hemp seed. Aliquots were examined for nonvolatile soluble solids, effect on CYP metabolism and P-glycoprotein (Pgp) transport, and major alpha- and beta-hop acids. Wide variance was detected in contents of alcohol, nonvolatile suspended solids, and hop acids and in the potential to affect CYP-mediated metabolism and Pgp-mediated efflux transport. Many of the products affected CYP2C9-mediated metabolism, and only two (NRP 306 and 307) markedly affected CYP3A4; hence, some products have the capacity to affect drug safety. CYP3A4, CYP3A5, CYP3A7, and CYP19 (aromatase) inhibition to the log concentration of beta-acid content was significant with r(2) > 0.37, suggesting that these components can account for some of the variation in inhibition of CYP metabolism.
Foster
Brian C
BC
Centre for Research in Biopharmaceuticals and Biotechnology, University of Ottawa, Ottawa, Ontario, Canada. brian_foster@hc-sc.gc.ca
Kearns
Nikia
N
Arnason
John T
JT
Saleem
Ammar
A
Ogrodowczyk
Carolina
C
Desjardins
Suzanne
S
D000085:Acetates / Q000032:analysis / Q000494:pharmacology*; D001515:Beer / Q000032:analysis*; D002460:Cell Line; D002478:Cells, Cultured; D065607:Cytochrome P-450 Enzyme Inhibitors; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism*; D006801:Humans; D009000:Monocytes / Q000737:chemistry / Q000187:drug effects / Q000201:enzymology; D011763:Pyrrolizidine Alkaloids / Q000032:analysis / Q000494:pharmacology*
C117972:(hexahydro-5-oxo-1H-pyrrolizinyl-1-yl)acetic acid; D000085:Acetates; D065607:Cytochrome P-450 Enzyme Inhibitors; D011763:Pyrrolizidine Alkaloids; D003577:Cytochrome P-450 Enzyme System