Recombinant S-layer proteins of Lactobacillus brevis mediating antibody adhesion to calf intestine alleviated neonatal diarrhea syndrome.
A chimeric gene encoding enhanced green fluorescent protein (EGFP) and a S-layer protein from Lactobacillus brevis KCTC3102, and/or two copies of the Fc-binding Z-domain, a synthetic analog of the B-domain of protein A, was constructed and expressed in Escherichia coli BL21(DE3). The S-layer fusion proteins produced in a 500-l fermentor were likely to be stable in the range of pH 5 to 8 and 0 degree to 40 degrees . Their adhesive property enabled an easy and rapid immobilization of enzymes or antibodies on solid materials such as plastics, glass, sol-gel films, and intestinal epithelial cells. Owing to their affinity towards intestinal cells and immunoglobulin G, the Slayer fusion proteins enabled the adhesion of antibodies to human epithelial cells. In addition, feeding a mixture of the S-layer fusion proteins and antibodies against neonatal calf diarrhea (coronavirus, rotavirus, Escherichia coli, and Salmonella typhimurium) to Hanwoo calves resulted in 100% prevention of neonatal calf diarrhea syndrome (p<0.01),whereas feeding antibodies only resulted in 56% prevention.
Khang
Yong-Ho
YH
School of Biotechnology, Yeungnam University, Gyeongsan 712-749, Korea. yhkhang@ynu.ac.kr
Park
Hee-Young
HY
Jeong
Yoo-Seok
YS
Kim
Jung-Ae
JA
Kim
Young-Hwan
YH
D000818:Animals; D000915:Antibody Affinity; D002417:Cattle; D002418:Cattle Diseases / Q000276:immunology* / Q000378:metabolism / Q000382:microbiology; D003967:Diarrhea / Q000276:immunology / Q000382:microbiology / Q000662:veterinary*; D004847:Epithelial Cells / Q000276:immunology / Q000378:metabolism; D049452:Green Fluorescent Proteins; D006801:Humans; D007141:Immunoglobulin Fc Fragments / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology; D007413:Intestinal Mucosa / Q000378:metabolism*; D007422:Intestines / Q000276:immunology; D052196:Levilactobacillus brevis / Q000235:genetics*; D008562:Membrane Glycoproteins / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology*; D051379:Mice; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000276:immunology; D013205:Staphylococcal Protein A / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology
D007141:Immunoglobulin Fc Fragments; D008562:Membrane Glycoproteins; D011993:Recombinant Fusion Proteins; C055764:S-layer proteins; D013205:Staphylococcal Protein A; D049452:Green Fluorescent Proteins