title: Detection of protein complex interactions via a Blue Native-PAGE retardation assay.
abstract: We describe the Blue Native (BN)-PAGE retardation assay for the detection of interactions of biomolecules with protein complexes. Potential interactors of proteins are included in the BN gel matrix, resulting in retardation of proteins that interact with the added molecule. After validation using the T-cell antigen receptor, we applied the assay for a general identification of dextran interactors in combination with mass spectroscopy. The proteomic screen revealed triosephosphate isomerase oligomer as a dextran-binding, high M(R) complex.
authors.affiliation: Department for Molecular Immunology, Max Planck-Institute for Immunobiology, 79108 Freiburg, Germany.
authors.forename: Mahima
authors.initials: M
authors.lastname: Swamy
authors.forename: Eszter
authors.initials: E
authors.lastname: Molnar
authors.forename: Thomas
authors.initials: T
authors.lastname: Bock
authors.forename: Damaris
authors.initials: D
authors.lastname: Bausch-Fluck
authors.forename: Bernd
authors.initials: B
authors.lastname: Wollscheid
authors.forename: Wolfgang W A
authors.initials: WW
authors.lastname: Schamel
mesh_terms: D000818:Animals; D002460:Cell Line; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D006801:Humans; D051379:Mice; D055503:Protein Multimerization; D011948:Receptors, Antigen, T-Cell / Q000032:analysis* / Q000378:metabolism; D012394:Rosaniline Dyes
chemical_list: D011948:Receptors, Antigen, T-Cell; D012394:Rosaniline Dyes; C004692:coomassie Brilliant Blue