In vitro culture of isolated islets of Langerhans: analysis of function.
In vitro culture has been well defined as a useful tool to improve survival and functionality of isolated islets of Langerhans. Evaluation of islet morphology and function is essential prior to use for experimental investigations. Novel techniques such as co-culture and the use of matrices have been shown to improve islet survival and functional viability, and can enhance the purity of the islet preparations, which is particularly important prior to experimental use or patient transplantation.
Nolan
Anna L
AL
Elixir Pharmaceuticals, 12 Emily Street, Cambridge, MA 02139, USA. anolan@elixirpharm.com
D000328:Adult; D000818:Animals; D002478:Cells, Cultured; D006801:Humans; D007515:Islets of Langerhans / Q000166:cytology* / Q000502:physiology*; D046509:Tissue Culture Techniques