title: Bioreduction of alpha-chloroacetophenone by whole cells of marine fungi.
abstract: The asymmetric reduction of 2-chloro-1-phenylethanone (1) by seven strains of marine fungi was evaluated and afforded (S)-(-)-2-chloro-1-phenylethanol with, in the best case, an enantiomeric excess of 50% and an isolated yield of 60%. The ability of marine fungi to catalyse the reduction was directly dependent on growth in artificial sea water-based medium containing a high concentration of Cl(-) (1.2 M). When fungi were grown in the absence of artificial sea water, no reduction of 1 by whole cells was observed. The biocatalytic reduction of 1 was more efficient at neutral rather than acidic pH values and in the absence of glucose as co-substrate.
authors.affiliation: Instituto de Química de São Carlos, Universidade de São Paulo, Av. Trabalhador Sãocarlense, 400, CP 780, São Carlos, SP, CEP 13560-970, Brazil.
authors.forename: Lenilson C
authors.initials: LC
authors.lastname: Rocha
authors.forename: Hercules V
authors.initials: HV
authors.lastname: Ferreira
authors.forename: Eli F
authors.initials: EF
authors.lastname: Pimenta
authors.forename: Roberto G S
authors.initials: RG
authors.lastname: Berlinck
authors.forename: Mirna H R
authors.initials: MH
authors.lastname: Seleghim
authors.forename: Darci C D
authors.initials: DC
authors.lastname: Javaroti
authors.forename: Lara D
authors.initials: LD
authors.lastname: Sette
authors.forename: Rafaella C
authors.initials: RC
authors.lastname: Bonugli
authors.forename: André L M
authors.initials: AL
authors.lastname: Porto
mesh_terms: D001711:Biotransformation; D003470:Culture Media / Q000737:chemistry; D005658:Fungi / Q000302:isolation & purification / Q000378:metabolism*; D010084:Oxidation-Reduction; D012623:Seawater / Q000382:microbiology*; D002721:omega-Chloroacetophenone / Q000378:metabolism*
chemical_list: D003470:Culture Media; D002721:omega-Chloroacetophenone