ITPase-deficient mice show growth retardation and die before weaning.
Inosine triphosphate pyrophosphatase (ITPase), the enzyme that hydrolyzes ITP and other deaminated purine nucleoside triphosphates to the corresponding purine nucleoside monophosphate and pyrophosphate, is encoded by the Itpa gene. In this study, we established Itpa knockout (KO) mice and used them to show that ITPase is required for the normal organization of sarcomeres in the heart. Itpa(-/-) mice died about 2 weeks after birth with features of growth retardation and cardiac myofiber disarray, similar to the phenotype of the cardiac alpha-actin KO mouse. Inosine nucleotides were found to accumulate in both the nucleotide pool and RNA of Itpa(-/-) mice. These data suggest that the role of ITPase in mice is to exclude ITP from the ATP pool, and the main target substrate of this enzyme is rITP. Our data also suggest that cardiomyopathy, which is mainly caused by mutations in sarcomeric protein-encoding genes, is also caused by a defect in maintaining the quality of the ATP pool, which is an essential requirement for sarcomere function.
Behmanesh
M
M
Division of Neurofunctional Genomics, Department of Immunobiology and Neuroscience, Medical Institute of Bioregulation, Kyushu University, Fukuoka 812-8582, Japan.
Sakumi
K
K
Abolhassani
N
N
Toyokuni
S
S
Oka
S
S
Ohnishi
Y N
YN
Tsuchimoto
D
D
Nakabeppu
Y
Y
D000199:Actins / Q000235:genetics / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D009202:Cardiomyopathies / Q000201:enzymology* / Q000235:genetics / Q000473:pathology; D005260:Female; D005838:Genotype; D006130:Growth Disorders / Q000201:enzymology* / Q000235:genetics / Q000401:mortality; D007292:Inosine Nucleotides / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D008969:Molecular Sequence Data; D009206:Myocardium / Q000473:pathology; D010641:Phenotype; D011755:Pyrophosphatases / Q000172:deficiency / Q000235:genetics / Q000502:physiology*; D012333:RNA, Messenger / Q000378:metabolism; D012518:Sarcomeres / Q000378:metabolism / Q000502:physiology; D014886:Weaning; D000097630:Inosine Triphosphatase
D000199:Actins; D000255:Adenosine Triphosphate; D007292:Inosine Nucleotides; D011755:Pyrophosphatases; D012333:RNA, Messenger; D000097630:Inosine Triphosphatase