title: Isolation of Candida dubliniensis from denture wearers.
abstract: Candida albicans is considered the most important Candida species able to cause oral infections in denture wearers. In recent years, Candida dubliniensis has emerged as a pathogenic yeast in humans. The close phenotypic similarities of C. albicans and C. dubliniensis have led to the misidentification of these species. In this work, our aim was to verify through PCR the presence of C. dubliniensis in palate and maxillary denture samples from 112 denture wearers presenting with or without denture-related stomatitis (DRS). C. dubliniensis was isolated at low rates from both palate (5.3 % and 10.7 %) and maxillary denture (5.3 % and 8.9 %) samples from wearers regardless of the presence of the disease. However, when C. dubliniensis was detected in individuals with DRS, it was always associated with C. albicans. In addition, our results showed that C. albicans was the most commonly identified candidal species in maxillary denture and hard palate samples from DRS patients (78.5 % and 89.2 %, respectively) as well as from controls (31.2 % and 28.5 %, respectively). In conclusion, C. dubliniensis was detected in the oral environment of denture wearers. The association of C. dubliniensis with C. albicans occurred in approximately 10 % of the DRS cases.
authors.affiliation: Department of Biological Sciences, Bauru School of Dentistry, University of São Paulo, Bauru, SP, Brazil.
authors.forename: Thaís Helena
authors.initials: TH
authors.lastname: Gasparoto
authors.affiliation: Department of Biological Sciences, Bauru School of Dentistry, University of São Paulo, Bauru, SP, Brazil.
authors.forename: Thiago José
authors.initials: TJ
authors.lastname: Dionísio
authors.affiliation: Department of Stomatology (Pathology), Bauru School of Dentistry, University of São Paulo, Bauru, SP, Brazil.
authors.forename: Carine Ervolino
authors.initials: CE
authors.lastname: de Oliveira
authors.affiliation: Department of Prosthodontics, Bauru School of Dentistry, University of São Paulo, Bauru, SP, Brazil.
authors.forename: Vinicius Carvalho
authors.initials: VC
authors.lastname: Porto
authors.affiliation: Department of Biological Sciences, Bauru School of Dentistry, University of São Paulo, Bauru, SP, Brazil.
authors.forename: Valéria
authors.initials: V
authors.lastname: Gelani
authors.affiliation: Department of Biological Sciences, Bauru School of Dentistry, University of São Paulo, Bauru, SP, Brazil.
authors.forename: Carlos Ferreira
authors.initials: CF
authors.lastname: Santos
authors.affiliation: Department of Biological Sciences, Bauru School of Dentistry, University of São Paulo, Bauru, SP, Brazil.
authors.forename: Ana Paula
authors.initials: AP
authors.lastname: Campanelli
authors.affiliation: Department of Stomatology (Pathology), Bauru School of Dentistry, University of São Paulo, Bauru, SP, Brazil.
authors.forename: Vanessa Soares
authors.initials: VS
authors.lastname: Lara
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002175:Candida / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D002180:Candidiasis, Oral / Q000382:microbiology*; D004271:DNA, Fungal / Q000737:chemistry / Q000302:isolation & purification; D003778:Dentures / Q000009:adverse effects; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D013282:Stomatitis, Denture / Q000382:microbiology*
chemical_list: D004271:DNA, Fungal