Production of two monomer structures containing medium-chain-length polyhydroxyalkanoates by beta-oxidation-impaired mutant of Pseudomonas putida KT2442.
Pseudomonas putida KT2442 produces medium-chain-length (MCL) polyhydroxyalkanoates (PHA) from fatty acids. When gene encoding 3-hydroxyacyl-CoA dehydrogenase which catalyzes long-chain-3-hydroxyacyl-CoA to 3-ketoacyl-CoA, was partially or completely deleted in P. putida KTOY08, the PHA accumulated was shown to contain only two different monomer structures dominated by a monomer of the same chain length as that of the fatty acids fed and another monomer two carbon atoms shorter. Among the PHA copolymers, P(44% 3HD-co-3HDD) containing 44% 3HD and 56% 3HDD was demonstrated to have a melting temperature T(m), an apparent heat of fusion DeltaH(m) and a Young's modulus E of 75 degrees C, 51 J g(-1) and 2.0 MPa, respectively, the highest among all the MCL PHA studied.
Ma
Long
L
Multidisciplinary Research Center, Shantou University, Shantou, Guangdong, China.
Zhang
Haifeng
H
Liu
Qian
Q
Chen
Jiong
J
Zhang
Jing
J
Chen
Guo-Qiang
GQ
D005798:Genes, Bacterial; D009154:Mutation / Q000235:genetics*; D010084:Oxidation-Reduction; D054813:Polyhydroxyalkanoates / Q000096:biosynthesis* / Q000737:chemistry*; D016958:Pseudomonas putida / Q000235:genetics* / Q000254:growth & development; D013379:Substrate Specificity
D054813:Polyhydroxyalkanoates