Klebsiella pneumoniae Carbapenemase-producing Enterobacteriaceae, Northeast Florida.
BACKGROUND
Since 2001 there have been several reported outbreaks due to carbapenem-resistant Klebsiella pneumoniae (Kp), particularly in the northeastern states.


METHODS
Carbapenemase-producing Enterobacteriaceae from healthcare facilities in Northeast Florida were phenotypically identified and confirmed using PCR amplification and sequencing of the blaKPC gene.


RESULTS
Results from PFGE analysis of these isolates demonstrated possible horizontal spread from two possible "outbreak" strains during the study period.


CONCLUSIONS
We present the first published cluster of Kp and Escherichia coli (Ec) cases in Florida carrying the KPC-2 or KPC-3 gene.
Halstead
Diane C
DC
Jacksonville Pathology Associates, PA and Baptist Health, Microbiology Laboratory, Memorial Hospital Jacksonville, Jacksonville, FL 32207, USA. s65@fdn.com
Sellen
Timothy J
TJ
Adams-Haduch
Jennifer M
JM
Dossenback
Deborah A
DA
Abid
Joan
J
Doi
Yohei
Y
Paterson
David L
DL
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D015780:Carbapenems / Q000378:metabolism; D004196:Disease Outbreaks; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000187:drug effects / Q000201:enzymology / Q000235:genetics; D004927:Escherichia coli Infections / Q000453:epidemiology / Q000382:microbiology; D005260:Female; D005431:Florida / Q000453:epidemiology; D005838:Genotype; D006801:Humans; D007710:Klebsiella Infections / Q000453:epidemiology* / Q000382:microbiology*; D007711:Klebsiella pneumoniae / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics; D008297:Male; D008826:Microbial Sensitivity Tests; D008875:Middle Aged; D055815:Young Adult; D001618:beta-Lactamases / Q000235:genetics / Q000378:metabolism*
D001426:Bacterial Proteins; D015780:Carbapenems; D001618:beta-Lactamases; C495220:beta-lactamase KPC-3, Klebsiella pneumoniae; C531581:carbapenemase-2, Klebsiella pneumoniae