Spontaneous cytokine production in children according to biological characteristics and environmental exposures.
BACKGROUND
Environmental factors are likely to have profound effects on the development of host immune responses, with serious implications for infectious diseases and inflammatory disorders such as asthma.


OBJECTIVE
This study was designed to investigate the effects of environmental exposures on the cytokine profile of children.


METHODS
The study involved measurement of T helper (Th) 1 (interferon-gamma), 2 [interleukin (IL)-5 and IL-13], and the regulatory cytokine IL-10 in unstimulated peripheral blood leukocytes from 1,376 children 4-11 years of age living in a poor urban area of the tropics. We also assessed the impact of environmental exposures in addition to biological characteristics recorded at the time of blood collection and earlier in childhood (0-3 years before blood collection).


RESULTS
The proportion of children producing IL-10 was greater among those without access to drinking water [p < 0.05, chi-square test, odds ratio (OR) = 1.67]. The proportion of children producing IL-5 and IL-10 (OR = 10.76) was significantly greater in households that had never had a sewage system (p < 0.05, trend test).


CONCLUSIONS
These data provide evidence for the profound effects of environmental exposures in early life as well as immune homeostasis in later childhood. Decreased hygiene (lack of access to clean drinking water and sanitation) in the first 3 years of life is associated with higher spontaneous IL-10 production up to 8 years later in life.
Figueiredo
Camila Alexandrina
CA
Instituto de Ciências da Saúde, Universidade Federal de Bahia, Salvador, Brazil. cavfigueiredo@gmail.com
Alcântara-Neves
Neuza Maria
NM
Veiga
Rafael
R
Amorim
Leila D
LD
Dattoli
Vitor
V
Mendonça
Lívia Ribeiro
LR
Junqueira
Samuel
S
Genser
Bernd
B
Santos
Mariese
M
de Carvalho
Lain Carlos Pontes
LC
Cooper
Philip J
PJ
Rodrigues
Laura
L
Barreto
Maurício L
ML
D000367:Age Factors; D002648:Child; D002675:Child, Preschool; D004781:Environmental Exposure; D004785:Environmental Pollutants / Q000633:toxicity*; D005260:Female; D006801:Humans; D007107:Immune System / Q000187:drug effects*; D007371:Interferon-gamma / Q000097:blood*; D016753:Interleukin-10 / Q000097:blood*; D018793:Interleukin-13 / Q000097:blood*; D015848:Interleukin-5 / Q000097:blood*; D008297:Male; D012722:Sewage / Q000009:adverse effects; D014881:Water Supply / Q000032:analysis
D004785:Environmental Pollutants; D018793:Interleukin-13; D015848:Interleukin-5; D012722:Sewage; D016753:Interleukin-10; D007371:Interferon-gamma