title: Development of an mlrA gene-directed TaqMan PCR assay for quantitative assessment of microcystin-degrading bacteria within water treatment plant sand filter biofilms.
abstract: We report for the first time a quantitative mlrA gene-directed TaqMan PCR assay for the rapid detection of microcystin-degrading bacteria. This was applied, in combination with 16S ribosomal DNA-directed quantitative PCR and denaturing gradient gel electrophoresis, to study virgin sand filter column biofilm development and to correlate mlrA gene abundance with microcystin removal efficiency.
authors.affiliation: Australian Water Quality Centre, South Australian Water Corporation, Adelaide, Australia. daniel.hoefel@sawater.com.au
authors.forename: Daniel
authors.initials: D
authors.lastname: Hoefel
authors.forename: Caroline M M
authors.initials: CM
authors.lastname: Adriansen
authors.forename: Magali A C
authors.initials: MA
authors.lastname: Bouyssou
authors.forename: Christopher P
authors.initials: CP
authors.lastname: Saint
authors.forename: Gayle
authors.initials: G
authors.lastname: Newcombe
authors.forename: Lionel
authors.initials: L
authors.lastname: Ho
mesh_terms: D001419:Bacteria / Q000302:isolation & purification*; D001426:Bacterial Proteins / Q000235:genetics*; D018441:Biofilms; D016000:Cluster Analysis; D015169:Colony Count, Microbial / Q000379:methods*; D016172:DNA Fingerprinting; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D052998:Microcystins / Q000378:metabolism*; D008969:Molecular Sequence Data; D009691:Nucleic Acid Denaturation; D016133:Polymerase Chain Reaction / Q000379:methods*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D018508:Water Purification
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D052998:Microcystins; D012336:RNA, Ribosomal, 16S; C078588:microcystin