Molecular cloning of the canine fragile histidine triad (FHIT) gene and Fhit protein expression in canine peripheral blood mononuclear cells.
A fragile histidine triad (FHIT) gene has been studied as a tumor-associated gene in humans. The aberrant FHIT gene and its protein expression have been reported in many types of human cancers. The present study explored the canine FHIT gene structure and its protein expression in the peripheral blood mononuclear cells of healthy dogs by RT-PCR, RACE and immunoblot analysis. The obtained canine FHIT gene contained nine small exons and was located on canine chromosome 20. Furthermore, we identified an alternative splicing form of the FHIT transcript. The deduced amino acid sequence was well conserved between species, and anti-human Fhit antibody could be used to detect the canine Fhit protein. These findings will be useful for future research.
Hiraoka
Hiroko
H
Faculty of Agriculture, Yamaguchi University, Yamaguchi, Japan.
Minami
Koji
K
Kaneko
Naoki
N
Shimokawa Miyama
Takako
T
Mizuno
Takuya
T
Okuda
Masaru
M
D017766:Acid Anhydride Hydrolases / Q000096:biosynthesis / Q000235:genetics*; D017398:Alternative Splicing; D000818:Animals; D002874:Chromosome Mapping / Q000662:veterinary; D003001:Cloning, Molecular; D004285:Dogs / Q000097:blood / Q000235:genetics*; D015151:Immunoblotting / Q000662:veterinary; D007963:Leukocytes, Mononuclear / Q000378:metabolism / Q000502:physiology*; D009363:Neoplasm Proteins / Q000096:biosynthesis / Q000235:genetics*; D019105:Random Amplified Polymorphic DNA Technique / Q000662:veterinary; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D000099170:Bis(5'-adenosyl)triphosphatase
D017766:Acid Anhydride Hydrolases; D009363:Neoplasm Proteins; D000099170:Bis(5'-adenosyl)triphosphatase