Utility of a protein fraction with cathepsin L-Like activity purified from cysticercus fluid of Taenia solium in the diagnosis of human cysticercosis.
Neurocysticercosis, an endemic parasitic disease in most developing countries, is caused by Taenia solium and compromises the human central nervous system. Cathepsin L-like proteases are secreted by several parasites including T. solium and constitute important antigens for immunodiagnostics. A protein fraction with cathepsin L-like activity was purified from the cysticercus fluid by size exclusion and ion exchange chromatography. Cathepsin L-like activity was measured fluorometrically by detecting the hydrolysis of the fluorogenic substrate Z-Phe-Arg-AMC. The purified protein fraction included antigens of 53 and 25 kD that were tested in a Western immunoblot and in an enzyme-linked immunosorbent assay (ELISA) for detection of human cysticercosis. The sensitivity of the Western immunoblot was 96% for patients infected with multiple cysts and 78% for patients with a single cyst. Specificity was 98%. The sensitivity of the ELISA was 98% in patients with multiple cysts and 84% in patients with a single cyst. Specificity was 92.7%.
Zimic
Mirko
M
Laboratorios de Investigación y Desarrollo. Facultad de Ciencias, Universidad Peruana Cayetano Heredia, Lima, Perú.
Pajuelo
Mónica
M
Rueda
Daniel
D
López
César
C
Arana
Yanina
Y
Castillo
Yesenia
Y
Calderón
Maritza
M
Rodriguez
Silvia
S
Sheen
Patricia
P
Vinetz
Joseph M
JM
Gonzales
Armando
A
García
Héctor H
HH
Gilman
Robert H
RH
D000818:Animals; D015153:Blotting, Western; D056668:Cathepsin L; D002403:Cathepsins / Q000276:immunology / Q000302:isolation & purification / Q000378:metabolism*; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D003546:Cysteine Endopeptidases / Q000276:immunology / Q000302:isolation & purification / Q000378:metabolism*; D003551:Cysticercosis / Q000175:diagnosis*; D003552:Cysticercus / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D015801:Helminth Proteins / Q000276:immunology / Q000302:isolation & purification / Q000378:metabolism*; D006801:Humans; D012680:Sensitivity and Specificity; D013552:Swine; D041201:Taenia solium / Q000378:metabolism*
D015801:Helminth Proteins; D002403:Cathepsins; D003546:Cysteine Endopeptidases; C534281:CTSL protein, human; D056668:Cathepsin L