The role of the catalytic domain of E. coli GluRS in tRNAGln discrimination.
Discrimination of tRNA(Gln) is an integral function of several bacterial glutamyl-tRNA synthetases (GluRS). The origin of the discrimination is thought to arise from unfavorable interactions between tRNA(Gln) and the anticodon-binding domain of GluRS. From experiments on an anticodon-binding domain truncated Escherichia coli (E. coli) GluRS (catalytic domain) and a chimeric protein, constructed from the catalytic domain of E. coli GluRS and the anticodon-binding domain of E. coli glutaminyl-tRNA synthetase (GlnRS), we show that both proteins discriminate against E. coli tRNA(Gln). Our results demonstrate that in addition to the anticodon-binding domain, tRNA(Gln) discriminatory elements may be present in the catalytic domain in E. coli GluRS as well.
Dasgupta
Saumya
S
Department of Biophysics, Bose Institute, P-1/12 CIT Scheme VIIM, Kolkata, India.
Saha
Rajesh
R
Dey
Chiranjeeb
C
Banerjee
Rajat
R
Roy
Siddhartha
S
Basu
Gautam
G
D000926:Anticodon / Q000378:metabolism; D001483:Base Sequence; D020134:Catalytic Domain; D002942:Circular Dichroism; D017931:DNA Primers / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics; D029968:Escherichia coli Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D005975:Glutamate-tRNA Ligase / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D007700:Kinetics; D008958:Models, Molecular; D011487:Protein Conformation; D017434:Protein Structure, Tertiary; D012329:RNA, Bacterial / Q000378:metabolism*; D012351:RNA, Transfer, Gln / Q000378:metabolism*; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D013050:Spectrometry, Fluorescence; D013379:Substrate Specificity; D046249:Transfer RNA Aminoacylation
D000926:Anticodon; D017931:DNA Primers; D004269:DNA, Bacterial; D029968:Escherichia coli Proteins; D012329:RNA, Bacterial; D012351:RNA, Transfer, Gln; D011994:Recombinant Proteins; D005975:Glutamate-tRNA Ligase