title: Development of an antibody hapten-chip system for detecting the residues of multiple antibiotic drugs.
abstract: The abuse of antibiotic drugs during animal production remains a worldwide problem and the subsequent detection of the residues of various drugs present at low concentrations in complex biological matrices poses significant analytical challenges. The present study outlines a practical biochip assay system to identify antibiotic residues in different animal tissue extracts. The system uses a simple but efficient multiresidue sample extraction procedure to isolate the antibiotic residues which were then identified directly using high-affinity monoclonal antibodies presented in a competitive immunoassay with conjugated antibiotic hapten-chips. The hapten-chip can analyze six samples each for eight antibiotics on a single chip within 3 h. The analytical results with both artificial positive standard samples and the incurred samples show that the antibody hapten-chip system has a comparable accuracy and a similar sensitivity to a standard ultra performance liquid chromatography-mass spectrometry (MS)/MS assay. In conclusion, an effective analytical screening system based on antibody hapten-chip was developed for detecting multiple antibiotic residues from multiple samples.
authors.affiliation: Medical Systems Biology Research Center, Tsinghua University School of Medicine, Beijing 100084, China.
authors.forename: Ailiang
authors.initials: A
authors.lastname: Chen
authors.forename: Guoqing
authors.initials: G
authors.lastname: Wang
authors.forename: Qin
authors.initials: Q
authors.lastname: Cao
authors.forename: Yan
authors.initials: Y
authors.lastname: Wang
authors.forename: Zhaohui
authors.initials: Z
authors.lastname: Zhang
authors.forename: Yimin
authors.initials: Y
authors.lastname: Sun
authors.forename: Hui
authors.initials: H
authors.lastname: Wang
authors.forename: Chaoyi
authors.initials: C
authors.lastname: Xu
authors.forename: Qi
authors.initials: Q
authors.lastname: Zhou
authors.forename: Pei
authors.initials: P
authors.lastname: Han
authors.forename: Miao
authors.initials: M
authors.lastname: Liu
authors.forename: Yang
authors.initials: Y
authors.lastname: Yang
authors.forename: Wanli
authors.initials: W
authors.lastname: Xing
authors.forename: Keith R
authors.initials: KR
authors.lastname: Mitchelson
authors.forename: Jing
authors.initials: J
authors.lastname: Cheng
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000032:analysis*; D000911:Antibodies, Monoclonal; D002645:Chickens; D002853:Chromatography, Liquid; D004350:Drug Residues; D006241:Haptens / Q000276:immunology*; D007118:Immunoassay / Q000379:methods*; D008099:Liver; D013058:Mass Spectrometry; D008461:Meat Products; D018482:Muscle, Skeletal; D013552:Swine; D046888:Tissue Array Analysis
chemical_list: D000900:Anti-Bacterial Agents; D000911:Antibodies, Monoclonal; D006241:Haptens