title: Connexin40 and connexin43 determine gating properties of atrial gap junction channels.
abstract: While ventricular gap junctions contain only Cx43, atrial gap junctions contain both Cx40 and Cx43; yet the functional consequences of this co-expression remain poorly understood. We quantitated the expression of Cx40 and Cx43 and their contributions to atrial gap junctional conductance (g(j)). Neonatal murine atrial myocytes showed similar abundances of Cx40 and Cx43 proteins, while ventricular myocytes contained at least 20 times more Cx43 than Cx40. Since Cx40 gap junction channels are blocked by 2 mM spermine while Cx43 channels are unaffected, we used spermine block as a functional dual whole cell patch clamp assay to determine Cx40 contributions to cardiac g(j). Slightly more than half of atrial g(j) and <or=20% of ventricular g(j) were inhibited. In myocytes from Cx40 null mice, the inhibition of ventricular g(j) was completely abolished, and the block of atrial g(j) was reduced to <20%. Compared to ventricular gap junctions, the transjunctional voltage (V(j))-dependent inactivation of atrial g(j) was reduced and kinetically slowed, while the V(j)-dependence of fast and slow inactivation was unchanged. We conclude that Cx40 and Cx43 are equally abundant in atrium and make similar contributions to atrial g(j). Co-expression of Cx40 accounts for most, but not all, of the differences in the V(j)-dependent gating properties between atrium and ventricle that may play a role in the genesis of slow myocardial conduction and arrhythmias.
authors.affiliation: Department of Pharmacology, SUNY Upstate Medical University, 750 East Adams Street, Syracuse, NY 13210, USA.
authors.forename: Xianming
authors.initials: X
authors.lastname: Lin
authors.forename: Joanna
authors.initials: J
authors.lastname: Gemel
authors.forename: Aaron
authors.initials: A
authors.lastname: Glass
authors.forename: Christian W
authors.initials: CW
authors.lastname: Zemlin
authors.forename: Eric C
authors.initials: EC
authors.lastname: Beyer
authors.forename: Richard D
authors.initials: RD
authors.lastname: Veenstra
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D002460:Cell Line; D002478:Cells, Cultured; D018031:Connexin 43 / Q000378:metabolism*; D017630:Connexins / Q000378:metabolism*; D004594:Electrophysiology; D017629:Gap Junctions / Q000378:metabolism*; D006325:Heart Atria / Q000166:cytology*; D015151:Immunoblotting; D007150:Immunohistochemistry; D007473:Ion Channels / Q000037:antagonists & inhibitors / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D008856:Microscopy, Fluorescence; D032383:Myocytes, Cardiac / Q000187:drug effects / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D013096:Spermine / Q000494:pharmacology
chemical_list: D018031:Connexin 43; D017630:Connexins; D007473:Ion Channels; D013096:Spermine