High-performance liquid chromatographic purification of oligomeric procyanidins, trimers up to nonamers, derived from the bark of Jatoba (Hymenaea courbaril).
Procyanidin oligomers with different degrees of polymerization (up to nonamers) were efficiently purified from the bark of Jatoba (Hymenaea courbaril) by using a recently developed chromatographic separation method. Purification relied on a hydrogen bonding interaction between phenolic hydroxyl groups of the procyanidins and polyethylene glycol (PEG)-coated resin in a packed column. The individual procyanidins were identified by using electrospray ionization mass spectrometry (ESI-MS) and verified by a thiolytic degradation analysis. Our results demonstrate that Jatoba bark contained a large amount of procyanidins from monomer to nonamers or higher polymers composed of only B-type linked units (flavan-3-ol units linked through C-4 to C-8 (or C-6)) of epicatechin (EC) without gallate esters.
Sasaki
Katsunori
K
Central Laboratories for Frontier Technology, Kirin Holdings Co., Ltd., Yokohama, Japan.
Matsukura
Yasuko
Y
Shijima
Kumiko
K
Miyake
Mika
M
Fujiwara
Daisuke
D
Konishi
Yutaka
Y
D001704:Biopolymers / Q000737:chemistry / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D052597:Hymenaea / Q000737:chemistry*; D024301:Plant Bark / Q000737:chemistry*; D044945:Proanthocyanidins / Q000737:chemistry / Q000302:isolation & purification*; D021241:Spectrometry, Mass, Electrospray Ionization; D016339:Spectrometry, Mass, Fast Atom Bombardment
D001704:Biopolymers; D044945:Proanthocyanidins