Influence of sterilization method on the bond strength of caries-affected dentin.
This study evaluated the effect of sterilization method on the bond strength of caries-affected dentin before artificial caries development and after restoration. Twelve bovine incisors were sectioned perpendicularly to their long axes at 7 mm from the amelodentinal junction. They were painted with acid-resistant nail varnish, except on an exposed coronal dentin area. Four groups were formed (n = 3) in accordance with the sterilization method used, before artificial caries development and after complete restoration: NE - no sterilization (control group); G - gamma-rays before and after; A - steam autoclave before and after; AG - steam autoclave before and gamma-rays after. For artificial caries development, dentin sections were immersed in BHI broth with S. mutans. After the soft carious tissue was removed, dentin was restored with Scotchbond Multi-Purpose and Filtek Z250. Next, the samples were sterilized in accordance with the methods described above and microtensile testing was performed. The data were analyzed by the Mann-Whitney test (p < 0.05). The G (22.7 MPa) and AG groups (16.3 MPa) were not statistically different from the NE group (17.5 MPa). Nevertheless, there were statistical differences between groups A (6.3 MPa) and NE, A and G, A and AG, G and AG. The bond strength of caries-affected dentin was not influenced by gamma-ray sterilization irrespective of whether the sterilization was performed before or after restoration.
Carvalho
Fabíola Galbiatti
FG
Piracicaba Dental School, State University of Campinas, SP, Brazil.
Gonçalves
Luciano Souza
LS
Carlo
Hugo Lemes
HL
Soares
Carlos José
CJ
Correr-Sobrinho
Lourenço
L
Puppin-Rontani
Regina Maria
RM
D000818:Animals; D002417:Cattle; D003188:Composite Resins / Q000627:therapeutic use*; D003731:Dental Caries / Q000628:therapy*; D003793:Dental Restoration, Permanent; D013242:Sterilization / Q000379:methods*; D013718:Tensile Strength
D003188:Composite Resins