title: Identification and expression pattern of putative odorant-binding proteins and chemosensory proteins in antennae of the Microplitis mediator (Hymenoptera: Braconidae).
abstract: The parasitoids of Cotton Bollworm Microplitis mediator (Hymenoptera: Braconidae) find their hosts through the odor released by stressed plants. In this study, preliminary characterization and isolation of cDNAs from male M. mediator antennal libraries identified 8 putative odorant-binding proteins (OBPs). Real-time polymerase chain reaction method was used to study the expression pattern of these isolated genes. Their gene expression profiles under a wide range of conditions indicated that only 4 OBP genes in M. mediator were antenna specific. The remaining 4 genes are either expressed ubiquitously or strictly regulated in specialized tissues or during different developmental stages. Some OBP genes were gender specific. These findings support that OBPs play dynamic roles during development of M. mediator and are likely to be involved in broader physiological functions.
authors.affiliation: State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, West Yuanmingyuan Road, Beijing, China.
authors.forename: Shuai
authors.initials: S
authors.lastname: Zhang
authors.forename: Yong-Jun
authors.initials: YJ
authors.lastname: Zhang
authors.forename: Hong-Hua
authors.initials: HH
authors.lastname: Su
authors.forename: Xi-Wu
authors.initials: XW
authors.lastname: Gao
authors.forename: Yu-Yuan
authors.initials: YY
authors.lastname: Guo
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D005260:Female; D018507:Gene Expression Regulation, Developmental; D006927:Hymenoptera / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D019476:Insect Proteins / Q000235:genetics / Q000378:metabolism*; D008297:Male; D008969:Molecular Sequence Data; D018035:Receptors, Odorant / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D012737:Sex Factors
chemical_list: D019476:Insect Proteins; D018035:Receptors, Odorant; C045648:odorant-binding protein