Selective and tunable gradient device for cell culture and chemotaxis study.
This article describes a microfluidic device for cell culture and chemotaxis studies under various temporal and spatial concentration gradients of the medium or chemoattractant. Vertical membranes formed using in situ fabrication are employed to avoid fluid flow inside the cell observation chamber. Thus, the medium and chemoattractants are primarily provided by diffusion, maintaining cell-cell communication via secreted factors. Neutrophils were used to demonstrate the capability of the device for chemotaxis research. Experiments exhibited successful migration up a concentration gradient of interleukin 8.
Kim
Dongshin
D
Department of Mechanical Engineering, University of Wisconsin, Madison, WI 53706, USA.
Lokuta
Mary A
MA
Huttenlocher
Anna
A
Beebe
David J
DJ
D002449:Cell Aggregation; D018929:Cell Culture Techniques / Q000295:instrumentation*; D002633:Chemotaxis; D002634:Chemotaxis, Leukocyte; D004058:Diffusion; D006801:Humans; D008567:Membranes, Artificial; D046210:Microfluidic Analytical Techniques; D009504:Neutrophils / Q000166:cytology / Q000378:metabolism
D008567:Membranes, Artificial