title: Cytotoxic and genotoxic effects of sodium hypochlorite on human peripheral lymphocytes in vitro.
abstract: Chlorination is widely used method in the disinfection of drinking and utility water worldwide. In this study, cytotoxic and genotoxic effects of sodium hypochlorite were investigated by the cytokinesis-block micronucleus assay and chromosomal aberration analysis on human peripheral lymphocytes in vitro. A significant increase in chromosomal aberration frequency was observed in all treatments of NaOCl (0.030, 0.065, 0.100, 0.25, 0.5, 1, 2, 4 mug/mL) at 24 and 48 h compared with the negative control and mitomycin C (MMC, 0.3 mug/mL), which was used as a positive control. NaOCl significantly increased the frequency of micronuclei in a dose dependent manner. The results showed that there was a significant correlation between NaOCl concentration and chromosomal aberration, micronuclei frequency, necrotic cells, apoptotic cells and binucleated cells.
authors.affiliation: Department of Biology, Faculty of Sciences and Arts, University of Kafkas, Kars, Turkey, suleymangul@hotmail.com.
authors.forename: Süleyman
authors.initials: S
authors.lastname: Gül
authors.forename: Asu
authors.initials: A
authors.lastname: Savsar
authors.forename: Zeynep
authors.initials: Z
authors.lastname: Tayfa