Reverse transfection using gold nanoparticles.
Reverse transfection from a solid surface has the potential to deliver genes into various types of cell and tissue more effectively than conventional methods of transfection. We present a method for reverse transfection using a gold colloid (GC) as a nanoscaffold by generating nanoclusters of the DNA/reagent complex on a glass surface, which could then be used for the regulation of the particle size of the complex and delivery of DNA into nuclei. With this method, we have found that the conjugation of gold nanoparticles (20 nm in particle size) to the pEGFP-N1/Jet-PEI complex resulted in an increase in the intensity of fluorescence of enhanced green fluorescent protein (EGFP) (based on the efficiency of transfection) from human mesenchymal stem cells (hMSCs), as compared with the control without GC. In this manner, we constructed a method for reverse transfection using GC to deliver genes into the cells effectively.
Yamada
Shigeru
S
National Institute of Advanced Industrial Science and Technology, 2-41-6 OMI, Koto-ku, Tokyo, 135-0064, Japan.
Fujita
Satoshi
S
Uchimura
Eiichiro
E
Miyake
Masato
M
Miyake
Jun
J
D017979:Gold Colloid; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000378:metabolism; D053768:Metal Nanoparticles; D008856:Microscopy, Fluorescence; D036103:Nanotechnology; D010316:Particle Size; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D014162:Transfection / Q000379:methods*
D017979:Gold Colloid; D011994:Recombinant Proteins; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins