Strong antibody responses to Mycobacterium tuberculosis PE-PGRS62 protein are associated with latent and active tuberculosis.
Mycobacterium tuberculosis has a unique family of PE-PGRS proteins with conserved N-terminal domains (PE) containing site-specific proline-glutamine residues and polymorphic GC-rich repetitive sequences (PGRS). Tuberculosis (TB) patients produce antibodies against some such proteins, but it is not clear whether these responses correlate with disease. Clinical groups with different mycobacterium exposure were studied for their seroreactivity to PE-PGRS17 and PE-PGRS62 proteins and their respective PE domains. There were minimal antibody responses against both PE domains and full-length PE-PGRS17, even in patients with active TB. However, patients with active and latent TB showed significantly higher PE-PGRS62-specific immunoglobulin G antibody responses than treated TB patients and mycobacterium-reactive TB contacts without latent infection. Latently infected persons had high anti-PE-PGRS62 responses but low responses to the 38-kDa antigen commonly used for TB serology, while treated TB cases showed the opposite response. Thus, patterns of seroreactivity to PE-PGRS62 correlate with clinical status and are associated with latent TB infection.
Koh
Kah Wee
KW
Department of Microbiology, National University of Singapore, Singapore.
Soh
Shu E
SE
Seah
Geok Teng
GT
D000595:Amino Acid Sequence; D000907:Antibodies, Bacterial / Q000097:blood* / Q000276:immunology*; D000995:Antitubercular Agents / Q000627:therapeutic use; D001426:Bacterial Proteins / Q000276:immunology*; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood / Q000276:immunology; D008969:Molecular Sequence Data; D009169:Mycobacterium tuberculosis / Q000276:immunology*; D016415:Sequence Alignment; D014376:Tuberculosis / Q000188:drug therapy / Q000276:immunology*
D000907:Antibodies, Bacterial; D000995:Antitubercular Agents; D001426:Bacterial Proteins; D007074:Immunoglobulin G