Crystallization and preliminary X-ray analysis of mouse RANK and its complex with RANKL.
The interaction between the TNF-family molecule receptor activator of NF-kappaB ligand (RANKL) and its receptor RANK induces osteoclast formation, activation and survival in the process of bone remodelling. RANKL-RANK also plays critical roles in T-cell/dendritic cell communication and lymph-node formation and in a variety of pathologic conditions such as tumour-cell migration and bone metastasis. Both the ectodomain of mouse RANKL and the extracellular domain of mouse RANK have been cloned, expressed and purified. Crystals of RANK alone and of RANK in complex with RANKL have been obtained that are suitable for structure determination.
Walter
Thomas S
TS
Division of Structural Biology and Oxford Protein Production Facility, The Wellcome Trust Centre for Human Genetics, The Henry Wellcome Building for Genomic Medicine, University of Oxford, Roosevelt Drive, Oxford OX3 7BN, England.
Liu
Changzhen
C
Huang
Peng
P
Zhang
Shiqian
S
Wedderburn
Lucy R
LR
Gao
Bin
B
Owens
Raymond J
RJ
Stuart
David I
DI
Tang
Peifu
P
Ren
Jingshan
J
D000595:Amino Acid Sequence; D000818:Animals; D003001:Cloning, Molecular; D003460:Crystallization; D003625:Data Collection; D004926:Escherichia coli / Q000235:genetics; D005822:Genetic Vectors; D005982:Glutathione Transferase / Q000378:metabolism; D006639:Histidine / Q000737:chemistry; D002479:Inclusion Bodies / Q000378:metabolism; D051379:Mice; D008969:Molecular Sequence Data; D008970:Molecular Weight; D017510:Protein Folding; D017434:Protein Structure, Tertiary; D053245:RANK Ligand / Q000737:chemistry / Q000378:metabolism*; D053246:Receptor Activator of Nuclear Factor-kappa B / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D012399:Rotation; D012995:Solubility; D013223:Statistics as Topic; D014961:X-Ray Diffraction
D053245:RANK Ligand; D053246:Receptor Activator of Nuclear Factor-kappa B; D011993:Recombinant Fusion Proteins; D006639:Histidine; D005982:Glutathione Transferase