Capillary electrophoresis determination of thiopurine methyl transferase activity in erythrocytes.
Thiopurine S-methyltransferase (TPMT) catalyzes methylation of thiopurine drugs (e.g. 6-mercaptopurine, azathioprine). Decreased activity of TPMT is associated with hematopoietic toxicity after administration of standard doses of the drugs. We developed capillary electrophoretic method for determination of TPMT enzyme activity in erythrocytes. Limit of quantification of the method is 1.5 micromol/L (S/N=6). The recovery of 6-methylmercaptopurine was 87.5-94.8%, imprecision value (as CV, n=10) was 1.68% (within-day) and 2.53% (between-day). Erythrocyte TPMT activities were measured in 60 healthy adult volunteers.
Tomkova
Jana
J
Laboratory for Inherited Metabolic Disorders, Department of Clinical Biochemistry, University Hospital and Palacky University Olomouc, Olomouc, Czech Republic. janatomkova@centrum.cz
Friedecky
David
D
Polynkova
Adriana
A
Adam
Tomas
T
D000328:Adult; D019075:Electrophoresis, Capillary / Q000379:methods*; D004912:Erythrocytes / Q000201:enzymology / Q000378:metabolism*; D006801:Humans; D008780:Methyltransferases / Q000378:metabolism*
D008780:Methyltransferases; C022745:thiopurine methyltransferase