Pro-apoptotic activities of novel synthetic quinones in human cancer cell lines.
Four novel quinonoid compounds are evaluated for their apoptotic potential against three cancer cell lines, i.e. MCF-7, HeLa and MG-63. APOPercentage assay was used for the screening of the compounds and it was established that the apoptotic potential of these compounds is cell line specific. The compound 2-(1',4'-benzoquinon-2'-yl)-5-hydroxy-7-methyl-1,4-naphthoquinone (S4) was found to be most active, which has a potential to kill approximately 99% of HeLa cancer cells in vitro after 72h at a concentration of 10microM. The compound's ability to induce specific markers of apoptosis in HeLa cells is further confirmed by DNA fragmentation and cell cycle assays.
Sagar
Sunil
S
Department of Chemistry, University of the Western Cape, Bellville, Cape Town, South Africa. sunil@sanbi.ac.za
Green
Ivan R
IR
D000970:Antineoplastic Agents / Q000138:chemical synthesis / Q000494:pharmacology*; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects*; D002166:Camptothecin / Q000494:pharmacology; D002453:Cell Cycle / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D053938:DNA Fragmentation; D004305:Dose-Response Relationship, Drug; D006367:HeLa Cells; D006801:Humans; D009369:Neoplasms / Q000473:pathology*; D011809:Quinones / Q000138:chemical synthesis / Q000494:pharmacology*; D013997:Time Factors
D000970:Antineoplastic Agents; D000972:Antineoplastic Agents, Phytogenic; D011809:Quinones; D002166:Camptothecin