title: Differential regulation of inducible nitric oxide synthase and cyclooxygenase-2 expression by superoxide dismutase in lipopolysaccharide stimulated RAW 264.7 cells.
abstract: Inducible nitric oxide synthase (iNOS) and cyclooxygenase 2 (COX-2) have been known to be involved in various pathophysiological processes such as inflammation. This study was performed to determine the regulatory function of superoxide dismutase (SOD) on the LPS-induced expression of iNOS, and COX-2 in RAW 264.7 cells. When a cell-permeable SOD, Tat- SOD, was added to the culture medium of RAW 264.7 cells, it rapidly entered the cells in a dose-dependent manner. Treatment of RAW 264.7 cells with Tat-SOD led to decrease in LPS-induced ROS generation. Pretreatment with Tat-SOD significantly inhibited LPS-induced expression of iNOS and NO production but had no effect on the expression of COX-2 and PGE((2)) production in RAW 264.7 cells. Tat-SOD inhibited LPS-induced NF-kappaB DNA binding activity, IkappaBalpha degradation and activation of MAP kinases. These data suggest that SOD differentially regulate expression of iNOS and COX-2 in LPS-stimulated RAW 264.7 cells.
authors.affiliation: Department of Biomedical Science, College of Natural Sciences, Hallym University, Chunchon 200-702, Korea.
authors.forename: Ji Ae
authors.initials: JA
authors.lastname: Lee
authors.forename: Ha Yong
authors.initials: HY
authors.lastname: Song
authors.forename: Sung Mi
authors.initials: SM
authors.lastname: Ju
authors.forename: Su Jin
authors.initials: SJ
authors.lastname: Lee
authors.forename: Hyung Joo
authors.initials: HJ
authors.lastname: Kwon
authors.forename: Won Sik
authors.initials: WS
authors.lastname: Eum
authors.forename: Sang Ho
authors.initials: SH
authors.lastname: Jang
authors.forename: Soo Young
authors.initials: SY
authors.lastname: Choi
authors.forename: Jin Seu
authors.initials: JS
authors.lastname: Park
mesh_terms: D000818:Animals; D002460:Cell Line; D051546:Cyclooxygenase 2 / Q000235:genetics* / Q000378:metabolism; D016207:Cytokines / Q000276:immunology; D005786:Gene Expression Regulation; D008070:Lipopolysaccharides / Q000276:immunology / Q000378:metabolism; D051379:Mice; D020929:Mitogen-Activated Protein Kinase Kinases / Q000378:metabolism; D016328:NF-kappa B / Q000378:metabolism; D009569:Nitric Oxide / Q000378:metabolism; D052247:Nitric Oxide Synthase Type II / Q000235:genetics* / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism*
chemical_list: D016207:Cytokines; D008070:Lipopolysaccharides; D016328:NF-kappa B; D017382:Reactive Oxygen Species; D009569:Nitric Oxide; D052247:Nitric Oxide Synthase Type II; D051546:Cyclooxygenase 2; D013482:Superoxide Dismutase; D020929:Mitogen-Activated Protein Kinase Kinases