Selective detection of antibodies in microstructured polymer optical fibers.
We demonstrate selective detection of fluorophore labeled antibodies from minute samples probed by a sensor layer of complementary biomolecules immobilized inside the air holes of microstructured Polymer Optical Fiber (mPOF). The fiber core is defined by a ring of 6 air holes and a simple procedure was applied to selectively capture either alpha-streptavidin or alpha-CRP antibodies inside these air holes. A sensitive and easy-to-use fluorescence method was used for the optical detection. Our results show that mPOF based biosensors can provide reliable and selective antibody detection in ultra small sample volumes.
Jensen
Jesper
J
Hoiby
Poul
P
Emiliyanov
Grigoriy
G
Bang
Ole
O
Pedersen
Lars
L
Bjarklev
Anders
A