The mycotoxin ochratoxin A inhibits DNA topoisomerase II and induces polyploidy in cultured CHO cells.
Ochratoxin A (OTA), a known nephrotoxin and carcinogenic mycotoxin, was investigated to examine its effectiveness to induce cytotoxicity and DNA damage (Comet assay), as well as its possible inhibition of topoisomerase II (topo II) catalytic activity in cultured Chinese hamster ovary (CHO) cells. The analysis of OTA-induced DNA strand breaks as well as the flow cytometric assessment of polyploidy has provided evidence that is consistent with the idea of a mixed mode of action of the mycotoxin: in addition to its genotoxic activity, OTA may also interfere with chromosome distribution during cell division.
Cosimi
Simona
S
Department of Cell Biology, Faculty of Biology, University of Seville, Av. Reina Mercedes 6, E-41012 Seville, Spain.
Orta
Luis
L
Mateos
Santiago
S
Cortés
Felipe
F
D000818:Animals; D016466:CHO Cells; D002478:Cells, Cultured; D020552:Comet Assay; D006224:Cricetinae; D003412:Cricetulus; D053960:DNA Breaks / Q000187:drug effects; D004249:DNA Damage / Q000187:drug effects; D004250:DNA Topoisomerases, Type II / Q000378:metabolism; D005434:Flow Cytometry; D009183:Mycotoxins / Q000633:toxicity*; D009793:Ochratoxins / Q000633:toxicity*; D011123:Polyploidy; D059005:Topoisomerase II Inhibitors
D009183:Mycotoxins; D009793:Ochratoxins; D059005:Topoisomerase II Inhibitors; C025589:ochratoxin A; D004250:DNA Topoisomerases, Type II