Effects of heating and glycation of beta-lactoglobulin on its recognition by IgE of sera from cow milk allergy patients.
beta-Lactoglobulin (beta-LG) is one of the cow's major milk proteins and the most abundant whey protein. This globular protein of about 18 kDa is folded, forming a beta-barrel (or calyx) structure. This structure is stabilized by two disulfide bonds and can be altered by heating above 65 degrees C. beta-LG is also one of the major allergens in milk. Heating is one of the most common technologic treatments applied during many milk transformations. During heating in the presence of reducing sugars, beta-LG is also submitted to the Maillard reaction, which at the first stage consists of the covalent fixation of sugars on the epsilon-amino groups of lysyl residues. The following steps are condensation and polymerization reactions leading to the formation of melanoidins (brown pigments). Despite the frequency of use of heating during milk transformation, the effects of heat-induced denaturation and of glycation of beta-LG on its recognition by IgE from cow's milk allergy (CMA) patients are not fully understood. The objectives of our work were to evaluate the effect of heat-induced denaturation of bovine beta-LG on binding of IgE from CMA patients and to determine the effect of moderate glycation on the degree of recognition by IgE. We showed that heat-induced denaturation (loss of tertiary and secondary structures) of beta-LG is associated with weaker binding of IgE from CMA patients. It was also shown that moderate glycation of beta-LG in early stages of Maillard reaction has only a small effect on its recognition by IgE, whereas a high degree of glycation has a clear "masking" effect on the recognition of epitopes. This demonstrates the importance of epsilon-amino groups of lysines in the definition of epitopes recognized by IgE.
Taheri-Kafrani
Asghar
A
UR 1268 Biopolymeres Interactions Assemblages, INRA, Nantes, France.
Gaudin
Jean-Charles
JC
Rabesona
Hanitra
H
Nioi
Claudia
C
Agarwal
Deepesh
D
Drouet
Martine
M
Chobert
Jean-Marc
JM
Bordbar
Abdol-Khalegh
AK
Haertle
Thomas
T
D000818:Animals; D002417:Cattle; D006358:Hot Temperature; D006801:Humans; D007073:Immunoglobulin E / Q000097:blood* / Q000276:immunology; D007782:Lactoglobulins / Q000737:chemistry* / Q000276:immunology*; D016269:Milk Hypersensitivity / Q000097:blood / Q000276:immunology*; D055601:Organic Chemistry Phenomena; D055550:Protein Stability
D007782:Lactoglobulins; D007073:Immunoglobulin E