title: Trans-cyclization of phosphatidylinositol catalyzed by phospholipase C from Streptomyces antibioticus.
abstract: Calcium-dependent phosphatidylinositol-specific phospholipase C from Streptomyces antibioticus (saPLC1) catalyzes the cleavage of phosphatidylinositol (PI) by an unusual mechanism involving a 1,6-cyclization with formation of inositol trans-1,6-cyclic phosphate (1,6-IcP), rather then inositol cis-1,2-cyclic phosphate (1,2-IcP). This conclusion has been reached based on the comparison of the released cyclic phosphate intermediate by the H16A mutant of saPLC1 with a genuine 1,6-IcP synthesized by a chemoenzymatic approach.
authors.affiliation: Department of Medicinal Chemistry and Pharmacognosy, The University of Illinois at Chicago, Chicago, Illinois 60612, USA.
authors.forename: Chuan
authors.initials: C
authors.lastname: Bai
authors.forename: Li
authors.initials: L
authors.lastname: Zhao
authors.forename: Mario
authors.initials: M
authors.lastname: Rebecchi
authors.forename: Ming-Daw
authors.initials: MD
authors.lastname: Tsai
authors.forename: Karol S
authors.initials: KS
authors.lastname: Bruzik
mesh_terms: D002384:Catalysis; D003500:Cyclization; D019906:Nuclear Magnetic Resonance, Biomolecular; D010716:Phosphatidylinositols / Q000737:chemistry / Q000378:metabolism*; D013303:Streptomyces antibioticus / Q000201:enzymology*; D013379:Substrate Specificity; D010738:Type C Phospholipases / Q000737:chemistry / Q000378:metabolism*
chemical_list: D010716:Phosphatidylinositols; D010738:Type C Phospholipases