title: Angiotensin-converting enzyme gene insertion/deletion polymorphism in Egyptian patients with myocardial infarction.
abstract: BACKGROUND
  This work aimed to test the association of the angiotensin-converting enzyme gene insertion/deletion (I/D) polymorphism with myocardial infarction.
  
  
  METHODS
  This study comprised 79 Egyptian myocardial infarction cases with a mean age of 54.4+/-9.9 years including 60 males and 19 females, plus 238 healthy unrelated individuals of nearly matched age and sex as a control group. For all subjects, DNA testing for the angiotensin-converting enzyme gene I/D polymorphism was done using PCR amplification for detection of both the D and I alleles followed by a second run PCR specific for the I allele for samples typed as DD in the first run.
  
  
  RESULTS
  Cases had a higher frequency of DD (29.1%) and ID (62.0%) genotypes than II (8.9%) genotype, with a higher frequency of D allele than I allele (64.4% vs. 33.6%). Compared to controls, cases had a significantly higher frequency of ID genotype (62.0% vs. 47.5%, p<0.05).This was more apparent among cases in the low risk group (p=0.002) than in the high risk group (p=0.041).
  
  
  CONCLUSIONS
  The angiotensin-converting enzyme gene I/D polymorphism is probably a risk factor for ischaemic heart disease among Egyptian cases, particularly if integrated with other environmental and genetic risk factors.
authors.affiliation: Department of Genetics, Faculty of Medicine, Mansoura University, Mansoura, Egypt. settin60@gmail.com
authors.forename: Ahmad
authors.initials: A
authors.lastname: Settin
authors.forename: Rizk
authors.initials: R
authors.lastname: Elbaz
authors.forename: Amr
authors.initials: A
authors.lastname: Abbas
authors.forename: Ayman
authors.initials: A
authors.lastname: Abd-Al-Samad
authors.forename: Ahmed
authors.initials: A
authors.lastname: Noaman
mesh_terms: D000328:Adult; D004534:Egypt; D005260:Female; D017353:Gene Deletion; D006801:Humans; D008297:Male; D008875:Middle Aged; D016254:Mutagenesis, Insertional; D009203:Myocardial Infarction / Q000235:genetics*; D007703:Peptidyl-Dipeptidase A / Q000235:genetics*; D011110:Polymorphism, Genetic
chemical_list: D007703:Peptidyl-Dipeptidase A