Peptidic sequence "HSEAETGPP" is recognized by the sera of pars planitis patients.
OBJECTIVE
HLA class II, p-36 protein, heat shock protein and retinal antigens have been associated with pars planitis (PP), but their participation in the development of the disease are unknown. A search for new molecules related to PP is necessary. This work focused on the identification of peptides recognized by PP patient sera using the phage display method.


METHODS
Sera of PP patients were used to isolate peptides fused to M13-phage pIII protein. The response of PP and healthy sera to peptides was determined by ELISA. PCR amplification and sequencing of peptide-encoding fragments from clones with high recognition by PP sera were used to characterize displayed peptides.


RESULTS
One hundred clones were randomly selected from a phage display library after three panning rounds using serum proteins from a PP patient. The immunologic response level of 100 clones selected were determined with a major number of patients, it was found that one clone was recognized stronger in PP patients sera than in healthy sera (PP vs. healthy; P < 0.05). The peptide-encoding region of this clone was sequenced and translated. The peptide sequence corresponded to HSEAETGPP. An identical amino acid sequence to HSEAETGPP is found in the human proline-rich transmembrane protein 2 which has not been related with eye diseases.


CONCLUSIONS
These results suggest that the peptide HSEAETGPP is associated with PP.
Castañeda-Sánchez
Jorge I
JI
Department of Biochemistry, Escuela Nacional de Ciencias Biológicas del Instituto Politécnico Nacional, Mexico.
Curiel-Quesada
Everardo
E
Pedrosa-Seres
Miguel
M
Cancino-Diaz
Mario E
ME
Rodríguez-Martínez
Sandra
S
Cancino-Diaz
Juan C
JC
D000595:Amino Acid Sequence; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D015868:Pars Planitis / Q000097:blood* / Q000276:immunology*; D019151:Peptide Library; D010455:Peptides / Q000737:chemistry* / Q000235:genetics / Q000276:immunology*; D016133:Polymerase Chain Reaction
D019151:Peptide Library; D010455:Peptides