[Osteogenic and adipogenic differentiation of bone marrow-derived mesenchymal stem cells in patients with aplastic anemia].
OBJECTIVE
To investigate the osteogenic and adipogenic difference of bone marrow-derived mesenchymal stem cells (MSCs) between patients with aplastic anemia (AA) and healthy volunteers and to explore the role of MSCs adipo-differentiation in the pathogenetic mechanism of AA.


METHODS
MSCs were isolated from bone marrow of patients with AA and healthy donors and expanded in vitro. MSCs derived from the AA patients and healthy volunteers were compared with respect to morphology, in vitro proliferation capacity, phenotype, differentiation ability and gene expression during differentiation.


RESULTS
The MSCs clones in the AA patients were (19.30 +/- 4.77)/(5 x 10(5) MNCs)7 days after culture, being significantly lower than those in the healthy volunteers, which was (47.72 +/- 3.46)/(5 x 10(5) MSCs) (P < 0.05). Compared with those the healthy donors, MSCs from the AA patients had similar proliferative capacity in the first 8 passages and then decreased in the following passages. MSCs from different sources had the same phenotype. MSCs from the AA patients could differentiate more easily into adipocytes but less easily and slower into osteoblasts than those from the healthy volunteers.


CONCLUSIONS
The increased adipogenic capacity and decreased osteogenic capacity of MSCs in AA patients may contribute to the development and progress of AA.
Wang
Hai-yan
HY
Department of Hematology, Huashan Hospital, Fudan University, Shanghai 200032, China.
Ding
Tian-ling
TL
Xie
Yi
Y
Xu
Xiao-ping
XP
Yu
Long
L
Chen
Tong
T
D017667:Adipocytes / Q000166:cytology; D000293:Adolescent; D000328:Adult; D000741:Anemia, Aplastic / Q000473:pathology*; D001854:Bone Marrow Cells / Q000166:cytology*; D002454:Cell Differentiation; D002478:Cells, Cultured; D005260:Female; D006801:Humans; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D008875:Middle Aged; D010006:Osteoblasts / Q000166:cytology; D055815:Young Adult