Biological methods for archiving and maintaining mutant laboratory mice. Part I: conserving mutant strains.
The mouse is now firmly established as the model organism of choice for scientists studying mammalian biology and human disease. Consequently, a plethora of novel, genetically altered (GA) mouse lines have been created. In addition, the output from the large scale mutagenesis programmes currently under way around the world will increase the collection of GA mouse strains still further. Because of the implications for animal welfare and the constraints on resources, it would be unreasonable to expect anything other than those strains essential for ongoing research programmes to be maintained as breeding colonies. Unfortunately, unless the redundant strains are preserved using robust procedures, which guarantee their recovery, they will be lost to future generations of researchers.This chapter describes some of the preservation methods currently used in laboratories around the world to archive novel mouse strains.
Fray
Martin D
MD
Frozen Embryo & Sperm Archive (FESA), Medical Research Council, Mammalian Genetics Unit, Harwell, Oxfordshire, UK.
D000822:Animal Husbandry / Q000379:methods*; D000818:Animals; D018070:Biological Specimen Banks; D015925:Cryopreservation; D004622:Embryo, Mammalian / Q000166:cytology / Q000502:physiology; D005260:Female; D008297:Male; D051379:Mice; D008817:Mice, Mutant Strains / Q000235:genetics*; D009865:Oocytes / Q000166:cytology / Q000502:physiology; D013094:Spermatozoa / Q000166:cytology / Q000502:physiology