Effect of diabetes mellitus and insulin therapy on bone density around osseointegrated dental implants: a digital subtraction radiography study in rats.
OBJECTIVE
To evaluate the effect of diabetes mellitus (DM) and insulin therapy on bone density around osseointegrated dental implants by digital subtraction radiography (DSR).


METHODS
Forty implants were placed in tibiae of 40 adult rats. After a healing period of 2 months, the animals were divided into four groups of 10 animals each: a 2-month control group, sacrificed at time (A), a diabetic group (D), an insulin-treated group (I) and a 4-month control group (C). During 2 months, group I received subcutaneous doses of insulin, whereas groups C and D received only saline. The animals in groups D, I and C were thereafter sacrificed. The glucose plasma levels (GPLs) were monitored throughout the experiment. Film radiographs were taken at implant surgery and on the day of sacrifice. The radiographs were digitized, and bone density in regions of osseointegration (OR) around the implants was evaluated by quantitative DSR between baseline and final images. Differences in shades of gray among the groups were assessed using ANOVA.


RESULTS
GPLs were within normal range for groups A, C and I and higher for group D. There was a significant difference in mean gray shade values in the OR of subtraction images between groups D (122+/-7) and I (136+/-5) (P<0.05) while there were no significant differences between control groups A (128+/-13) and C (134+/-10) and the insulin group I.


CONCLUSIONS
DM impaired bone density around osseointegrated dental implant. Further, insulin therapy maintained bone density in diabetic rats.
de Morais
Juliana Aparecida Najarro Dearo
JA
Department of Oral Diagnosis and Surgery, Araraquara Dental School, São Paulo State University - UNESP, São Paulo, SP, Brazil. julianadearomorais@yahoo.com.br
Trindade-Suedam
Ivy Kiemle
IK
Pepato
Maria Teresa
MT
Marcantonio
Elcio
E
Wenzel
Ann
A
Scaf
Gulnara
G
D000539:Alveolar Process / Q000000981:diagnostic imaging* / Q000601:surgery*; D000704:Analysis of Variance; D000818:Animals; D015519:Bone Density / Q000187:drug effects* / Q000502:physiology*; D003758:Dental Implantation, Endosseous; D015921:Dental Implants; D003921:Diabetes Mellitus, Experimental / Q000188:drug therapy* / Q000503:physiopathology*; D007328:Insulin / Q000494:pharmacology*; D008297:Male; D016348:Osseointegration; D010012:Osteogenesis / Q000187:drug effects* / Q000502:physiology*; D011859:Radiography; D051381:Rats; D017208:Rats, Wistar; D013382:Subtraction Technique
D015921:Dental Implants; D007328:Insulin