Biotransformation of daidzein to equol by crude enzyme from Asaccharobacter celatus AHU1763 required an anaerobic environment.
Asaccharobacter celatus AHU1763 is a Gram-positive, obligate anaerobic, non-spore forming, rod-shaped bacteria that was successfully isolated from rat cecal content. Daizein was converted to equol via dihydrodaidzein by this bacterium. A crude enzyme that converted daidzein to dihydrodaidzein was detected mainly in the culture supernatant. The ability of this enzyme dropped after the culture supernatant was exposed to a normal atmospheric environment for even 5 min. Furthermore, the enzyme responsible for changing dihydrodaidzein to equol was detected mainly in the cell debris, which required anaerobic conditions for its activity.
Thawornkuno
Charin
C
Division of Applied Bioscience, Graduate School of Agriculture, Hokkaido University, Sapporo, Hokkaido, Japan.
Tanaka
Michiko
M
Sone
Teruo
T
Asano
Kozo
K
D000693:Anaerobiosis; D001711:Biotransformation; D060754:Equol; D006094:Gram-Positive Bacteria / Q000201:enzymology* / Q000254:growth & development; D006863:Hydrogen-Ion Concentration; D007529:Isoflavones / Q000493:pharmacokinetics*; D013696:Temperature
C007610:4',7-dihydroxy-3,4-dihydroisoflavone; D007529:Isoflavones; D060754:Equol; C004742:daidzein