title: Modulation of O-mediated retrograde signaling by the PLEIOTROPIC RESPONSE LOCUS 1 (PRL1) protein, a central integrator of stress and energy signaling.
abstract: Shortly after the release of singlet oxygen ((1)O(2)) in chloroplasts, changes in nuclear gene expression occur in the conditional flu mutant of Arabidopsis that reveal a rapid transfer of signals from the plastid to the nucleus. Extensive genetic screens aimed at identifying constituents involved in (1)O(2)-mediated plastid-to-nucleus signaling have failed to identify extraplastidic signaling components. This finding suggests that (1)O(2)-mediated signals are not translocated to the nucleus via a single linear pathway, but rather through a signaling network that is difficult to block by single mutations. The complexity of this signaling network has been tackled by mutagenizing a transgenic flu line expressing the luciferase reporter gene under the control of the promoter of a (1)O(2)-responsive AAA-ATPase gene (At3g28580) and isolating second site mutants that constitutively express the reporter gene at a high level. One of the mutants was shown by map-based cloning and sequencing to contain a single amino acid change in the PLEIOTROPIC RESPONSE LOCUS 1 (PRL1) protein. PRL1 suppresses the expression of AAA-ATPase and other (1)O(2)-responsive genes. PRL1 seems to play a major role in modulating responses of plants to environmental changes by interconnecting (1)O(2)-mediated retrograde signaling with other signaling pathways.
authors.affiliation: Institute of Plant Sciences, ETH Zurich, Zurich, Switzerland.
authors.forename: Aiswarya
authors.initials: A
authors.lastname: Baruah
authors.forename: Klára
authors.initials: K
authors.lastname: Simková
authors.forename: Dirk K
authors.initials: DK
authors.lastname: Hincha
authors.forename: Klaus
authors.initials: K
authors.lastname: Apel
authors.forename: Christophe
authors.initials: C
authors.lastname: Laloi
mesh_terms: D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D003001:Cloning, Molecular; D018506:Gene Expression Regulation, Plant; D005816:Genetic Complementation Test; D016296:Mutagenesis; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism*; D045332:Photosystem II Protein Complex; D030821:Plants, Genetically Modified / Q000235:genetics / Q000378:metabolism; D018749:RNA, Plant / Q000235:genetics; D015398:Signal Transduction; D026082:Singlet Oxygen / Q000378:metabolism*; D013312:Stress, Physiological
chemical_list: D029681:Arabidopsis Proteins; D002352:Carrier Proteins; D009687:Nuclear Proteins; C492005:PRL1 protein, Arabidopsis; D045332:Photosystem II Protein Complex; D018749:RNA, Plant; D026082:Singlet Oxygen