Metabolomic evaluation of pulsed electric field-induced stress on potato tissue.
Metabolite profiling was used to characterize stress responses of potato tissue subjected to reversible electroporation, providing insights on how potato tissue responds to a physical stimulus such as pulsed electric fields (PEF), which is an artificial stress. Wounded potato tissue was subjected to field strengths ranging from 200 to 400 V/cm, with a single rectangular pulse of 1 ms. Electroporation was demonstrated by propidium iodide staining of the cell nucleae. Metabolic profiling of data obtained through GC/TOF-MS and UPLC/TOF-MS complemented with orthogonal projections to latent structures clustering analysis showed that 24 h after the application of PEF, potato metabolism shows PEF-specific responses characterized by the changes in the hexose pool that may involve starch and ascorbic acid degradation.
Galindo
Federico Gómez
FG
Centro de Engenharia Biológica, Institute for Biotechnology and Bioengineering, Universidade do Minho, Campus de Gualtar, 4710-057, Braga, Portugal. Federico.Gomez@food.lth.se
Dejmek
Petr
P
Lundgren
Krister
K
Rasmusson
Allan G
AG
Vicente
António
A
Moritz
Thomas
T
D002849:Chromatography, Gas; D002853:Chromatography, Liquid; D016000:Cluster Analysis; D004560:Electricity; D018274:Electroporation; D013058:Mass Spectrometry; D008856:Microscopy, Fluorescence; D011198:Solanum tuberosum / Q000378:metabolism* / Q000502:physiology; D013312:Stress, Physiological