Recovery of anti-Toxoplasma gondii immunoglobulin M in stored guthrie card blood spots.
Retrospective testing of neonatal Guthrie card blood spots for specific immunoglobulin M (IgM) can distinguish congenital toxoplasmosis from acquired toxoplasmosis. We determined whether storage temperature reduced IgM detection, using filter paper blood samples "spiked" with anti-Toxoplasma IgM. After 300 days, IgM detection deteriorated with storage at room temperature but not at temperatures of 4 degrees C or lower.
Tan
Hooi-Kuan
HK
MRC Centre of Epidemiology for Child Health, Institute of Child Health, London, United Kingdom.
Petersen
Eskild
E
Møller
Lone N
LN
Phillips
Pamela
P
Neto
Eurico Camargo
EC
Gilbert
Ruth E
RE
D000293:Adolescent; D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology*; D001769:Blood / Q000276:immunology*; D002648:Child; D002675:Child, Preschool; D003890:Desiccation; D006801:Humans; D007075:Immunoglobulin M / Q000276:immunology*; D007223:Infant; D007231:Infant, Newborn; D012034:Refrigeration; D013048:Specimen Handling / Q000379:methods*; D013696:Temperature; D013997:Time Factors; D014122:Toxoplasma / Q000276:immunology*; D014123:Toxoplasmosis / Q000175:diagnosis*
D000907:Antibodies, Bacterial; D007075:Immunoglobulin M