Two new spirobiflavonoids from Abies chensiensis with moderate NO production inhibitory activity.
Phytochemical investigation of the aerial parts of Abies chensiensis afforded two new (compounds 2 and 3) and 27 known compounds, including the related compound larixinol ( 1). The structures of spirobiflavonoids 1- 3 were established using 1D and 2D NMR spectroscopic techniques. In addition, the structure of larixinol ( 1) was confirmed by X-ray crystallographic analysis. Compounds 1- 3 were evaluated for inhibitory activities against LPS-induced NO production in macrophages. Larixinol ( 1) showed moderate effects, with an IC(50) value of 60.0 microg/mL. In addition, it did not show any cytotoxicity on RAW 264.7 macrophages at 100 microg/mL.
Li
Yong-Li
YL
Department of Natural Product Chemistry, School of Pharmacy, Second Military Medical University, Shanghai, P. R. China.
Yang
Xian-Wen
XW
Li
Su-Mei
SM
Tang
Jian
J
Tian
Jun-Mian
JM
Peng
Xiao-Yang
XY
Huang
Da-Seng
DS
Zhang
Wei-Dong
WD
D028202:Abies / Q000737:chemistry*; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000302:isolation & purification / Q000494:pharmacology*; D002460:Cell Line; D018360:Crystallography, X-Ray; D005419:Flavonoids / Q000302:isolation & purification / Q000494:pharmacology*; D020128:Inhibitory Concentration 50; D008070:Lipopolysaccharides; D008264:Macrophages / Q000187:drug effects*; D009682:Magnetic Resonance Spectroscopy; D051379:Mice; D009569:Nitric Oxide / Q000096:biosynthesis*; D035261:Plant Components, Aerial; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D013141:Spiro Compounds / Q000302:isolation & purification / Q000494:pharmacology*
D000893:Anti-Inflammatory Agents; D005419:Flavonoids; D008070:Lipopolysaccharides; D010936:Plant Extracts; D013141:Spiro Compounds; C548292:larixinol; D009569:Nitric Oxide