Sediment genotoxicity in the Tietê River (São Paulo, Brazil): in vitro comet assay versus in situ micronucleus assay studies.
The in vitro comet assay with the permanent fish cell line RTL-W1 and the in situ micronucleus assay using erythrocytes from indigenous tilapia (Oreochromis niloticus) were used to detect genotoxicity in Tietê River sediments (São Paulo, Brazil). Either test was successful in identifying site-specific differences in genotoxicity, with a high correlation between in situ and in vitro results indicating the relevance of the latter even for environmental studies. Discharges from São Paulo city have major impact on genotoxic effects by sediment-bound contaminants; however, overall genotoxicity decreases downstream. The high genotoxic burden of the Tietê River warrants measures to reduce the input of toxic effluents.
Rocha
Paula Suares
PS
Aquatic Ecology and Toxicology Section, Heidelberg Institute of Zoology, University of Heidelberg, Im Neuenheimer Feld 230, 69120 Heidelberg, Germany. rocha@zoo.uni-heidelberg.de
Luvizotto
George Luiz
GL
Kosmehl
Thomas
T
Böttcher
Melanie
M
Storch
Volker
V
Braunbeck
Thomas
T
Hollert
Henner
H
D000818:Animals; D001938:Brazil; D002460:Cell Line; D020552:Comet Assay; D004249:DNA Damage; D004784:Environmental Monitoring / Q000379:methods*; D004912:Erythrocytes / Q000187:drug effects; D005347:Fibroblasts / Q000187:drug effects; D019015:Geologic Sediments / Q000737:chemistry*; D008099:Liver / Q000166:cytology; D048629:Micronuclei, Chromosome-Defective / Q000139:chemically induced*; D015162:Micronucleus Tests; D017686:Oncorhynchus mykiss; D045483:Rivers / Q000737:chemistry*; D017210:Tilapia / Q000097:blood; D014874:Water Pollutants, Chemical / Q000633:toxicity*
D014874:Water Pollutants, Chemical