Sequence, expression divergence, and complementation of homologous ALCATRAZ loci in Brassica napus.
The genomic era provides new perspectives in understanding polyploidy evolution, mostly on the genome-wide scale. In this paper, we show the sequence and expression divergence between the homologous ALCATRAZ (ALC) loci in Brassica napus, responsible for silique dehiscence. We cloned two homologous ALC loci, namely BnaC.ALC.a and BnaA.ALC.a in B. napus. Driven by the 35S promoter, both the loci complemented to the alc mutation of Arabidopsis thaliana, yet only the expression of BnaC.ALC.a was detectable in the siliques of B. napus. Sequence alignment indicated that BnaC.ALC.a and BolC.ALC.a, or BnaA.ALC.a and BraA.ALC.a, possess a high level of similarity. The understanding of the sequence and expression divergence among homologous loci of a gene is of due importance for an effective gene manipulation and TILLING (or ECOTILLING) analysis for the allelic DNA variation at a given locus.
Hua
Shuijin
S
Institute of Crop Science, College of Agriculture and Biotechnology, Zhejiang University, 268 Kaixuan Road, 310029, Hangzhou, People's Republic of China.
Shamsi
Imran Haider
IH
Guo
Yuan
Y
Pak
Haksong
H
Chen
Mingxun
M
Shi
Congguang
C
Meng
Huabing
H
Jiang
Lixi
L
D000595:Amino Acid Sequence; D001483:Base Sequence; D029688:Brassica napus / Q000235:genetics*; D017343:Genes, Plant; D005816:Genetic Complementation Test; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000235:genetics*; D011123:Polyploidy; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid
D010940:Plant Proteins