title: Cacao procyanidins reduce plasma cholesterol and increase fecal steroid excretion in rats fed a high-cholesterol diet.
abstract: Cocoa powder is rich in polyphenols, such as catechins and oligomeric procyanidins, and has a hypocholesterolemic effect in humans. This study evaluated the principal active components and potential mechanism(s) for the hypocholesterolemic effect of polyphenolic substances from cocoa powder in rats. Male Wistar rats were fed a 1% high-cholesterol diet (HC) or a high-cholesterol diet containing 1% polyphenol extract from cocoa powder (PE) or a mixture of 0.024% catechin and 0.058% epicatechin (CE) for 4 weeks. We also examined the effects of these polyphenolic substances on micellar cholesterol solubility in vitro. The PE group had significantly lower plasma cholesterol concentrations, and had significantly greater fecal cholesterol and total bile acids excretion than the HC group. The CE group diet did not influence plasma cholesterol concentrations, or fecal cholesterol or total bile acids excretion. Micellar solubility of cholesterol in vitro was significantly lower for procyanidin B2 (dimer), B5 (dimer), C1 (trimer) and A2 (tetramer), which are the main components of polyphenol extract from cocoa powder, compared to catechin and epicatechin. These results suggest that oligomeric procyanidins from cocoa powder are the principal active components responsible for the hypocholesterolemic effect, and inhibit the intestinal absorption of cholesterol and bile acids through the decrease in micellar cholesterol solubility.
authors.affiliation: Food and Health R&D Laboratories, Meiji Seika Kaisha Ltd., Saitama, Japan.
authors.forename: Akiko
authors.initials: A
authors.lastname: Yasuda
authors.forename: Midori
authors.initials: M
authors.lastname: Natsume
authors.forename: Kazue
authors.initials: K
authors.lastname: Sasaki
authors.forename: Seigo
authors.initials: S
authors.lastname: Baba
authors.forename: Yuko
authors.initials: Y
authors.lastname: Nakamura
authors.forename: Minoru
authors.initials: M
authors.lastname: Kanegae
authors.forename: Satoshi
authors.initials: S
authors.lastname: Nagaoka
mesh_terms: D000818:Animals; D002099:Cacao / Q000737:chemistry*; D002784:Cholesterol / Q000097:blood; D002791:Cholesterol, Dietary / Q000008:administration & dosage; D005243:Feces / Q000737:chemistry; D008055:Lipids / Q000097:blood; D008297:Male; D044945:Proanthocyanidins / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D013256:Steroids / Q000378:metabolism; D002785:Sterol O-Acyltransferase / Q000378:metabolism
chemical_list: D002791:Cholesterol, Dietary; D008055:Lipids; D044945:Proanthocyanidins; D013256:Steroids; D002784:Cholesterol; D002785:Sterol O-Acyltransferase; C493492:sterol O-acyltransferase 1