Development of an analytical methodology for simultaneous determination of vincristine and doxorubicin in pharmaceutical preparations for oncology by HPLC-UV.
A high-performance liquid chromatography-UV methodology (lambda=230 nm) was developed and validated for the simultaneous determination of vincristine and doxorubicin in pharmaceutical preparations used in oncology. The chromatography was carried out on a C18 column using acetonitrile 90% in water-potassium hydrogenphosphate buffer 50 mM, pH 3.2+/-0.1 (32:68, v/v) as mobile phase at a flow rate of 1.5 mL/min. The method proved to be specific, exact, and accurate, in accordance with the ICH standards, presenting linearity in the 1-5 microg/mL and 5-100 microg/mL intervals, and detection (0.19x0.51 microg/mL) and quantification (0.63x1.7 microg/mL) limits for vincristine and doxorubicin, respectively.
Rodrigues
Ana Sofia
AS
Grupo de Bromatologia-Centro de Estudos Farmacêuticos, Faculdade de Farmácia da Universidade de Coimbra, 3000-295 Coimbra, Portugal.
Lopes
Ana Rita
AR
Leão
Ana
A
Couceiro
Alda
A
Ribeiro
Ana Bela Sarmento
AB
Ramos
Fernando
F
Noronha da Silveira
Maria Irene
MI
Resende de Oliveira
Catarina
C
D000970:Antineoplastic Agents / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004317:Doxorubicin / Q000032:analysis*; D006863:Hydrogen-Ion Concentration; D004364:Pharmaceutical Preparations / Q000737:chemistry*; D012015:Reference Standards; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet / Q000379:methods*; D014750:Vincristine / Q000032:analysis*
D000970:Antineoplastic Agents; D004364:Pharmaceutical Preparations; D014750:Vincristine; D004317:Doxorubicin