[Study on the inducement and influential factors of callus with different organs of Glycyrrhiza uralensis].
OBJECTIVE
To investigate the influential factors and establish culture method for G. uralensis callus.


METHODS
To study the possible effective factors of culture condition by comparing with different explants, light, plant hormones and its ratio.


RESULTS
The hypocotyl was the best among different explants, its inducing ratios was 94%, and the callus occurred earliest. When the calluses were inoculated on MS + 6 - BA (1.0-2.0) mg/L + NAA (0.5-1.0) mg/L, adventitious buds formed. 2,4-D could induce non-embryogenic callus, the compounding proportions of 6 - BA and NAA could induce embryogenic callus. Light influenced induction and growth of callus.


CONCLUSIONS
Different explants, components of hormone and light are the influencial factors of callus induction and growth of G. uralensis.
Fan
Xiao-feng
XF
Department of Life Science, Longdong University, Qingyang 745000, China. qyszfxf@126.com
Yang
Ying-li
YL
Guo
Xiao-qiang
XQ
Liu
Xiu-li
XL
D015084:2,4-Dichlorophenoxyacetic Acid / Q000494:pharmacology; D003470:Culture Media; D029882:Glycyrrhiza uralensis / Q000145:classification / Q000187:drug effects / Q000254:growth & development*; D018546:Hypocotyl / Q000187:drug effects / Q000254:growth & development; D010937:Plant Growth Regulators / Q000494:pharmacology*; D018520:Plant Shoots / Q000187:drug effects / Q000254:growth & development; D010946:Plants, Medicinal / Q000187:drug effects / Q000254:growth & development*; D012639:Seeds / Q000187:drug effects / Q000254:growth & development; D013472:Sunlight; D046509:Tissue Culture Techniques / Q000379:methods*
D003470:Culture Media; D010937:Plant Growth Regulators; D015084:2,4-Dichlorophenoxyacetic Acid