Human extracellular matrix (ECM) powders for injectable cell delivery and adipose tissue engineering.
Here, we present extracellular matrix (ECM) powders derived from human adipose tissue as injectable cell delivery carriers for adipose tissue engineering. We postulate that human adipose tissue may provide an ideal biomaterial because it contains large amounts of ECM components including collagen. Fresh human adipose tissue was obtained by a simple surgical operation (liposuction). After removing blood and oil components, the tissue was homogenized, centrifuged, freeze-dried, and ground to powders by milling. In an in vitro study, the human ECM powders were highly effective for promotion of cell attachment and proliferation for three-dimensional (3D) cell culture. In in vivo studies, suspensions of human ECM powders containing human adipose-derived stem cells (hASCs) were subcutaneously injected into nude mice. At eight weeks post-injection, numerous blood vessels were observed and the newly formed tissue exhibited adipogenesis with accumulated intracellular small lipid droplets. Overall, the grafts showed well-organized adipose tissue constructs without any signs of tissue necrosis, cystic spaces, or fibrosis. We believe that human ECM powders could act as efficient injectable biomaterials for tissue engineering and have great potential for meeting clinical challenges in regenerative medicine, particularly in relation to adipose tissue engineering.
Choi
Ji Suk
JS
Department of Chemical Engineering, Hanyang University, Ansan, Gyeonggi-do 426-791, Republic of Korea.
Yang
Hyun-Jin
HJ
Kim
Beob Soo
BS
Kim
Jae Dong
JD
Kim
Jun Young
JY
Yoo
Bongyoung
B
Park
Kinam
K
Lee
Hee Young
HY
Cho
Yong Woo
YW
D017667:Adipocytes / Q000502:physiology*; D000273:Adipose Tissue / Q000737:chemistry*; D000328:Adult; D000818:Animals; D002448:Cell Adhesion; D049109:Cell Proliferation; D002469:Cell Separation; D004337:Drug Carriers; D016503:Drug Delivery Systems; D005109:Extracellular Matrix / Q000737:chemistry*; D006801:Humans; D051379:Mice; D008855:Microscopy, Electron, Scanning; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013234:Stem Cells / Q000502:physiology*; D023822:Tissue Engineering; D055815:Young Adult
D004337:Drug Carriers