title: Metabolomic evaluation of pulsed electric field-induced stress on potato tissue.
abstract: Metabolite profiling was used to characterize stress responses of potato tissue subjected to reversible electroporation, providing insights on how potato tissue responds to a physical stimulus such as pulsed electric fields (PEF), which is an artificial stress. Wounded potato tissue was subjected to field strengths ranging from 200 to 400 V/cm, with a single rectangular pulse of 1 ms. Electroporation was demonstrated by propidium iodide staining of the cell nucleae. Metabolic profiling of data obtained through GC/TOF-MS and UPLC/TOF-MS complemented with orthogonal projections to latent structures clustering analysis showed that 24 h after the application of PEF, potato metabolism shows PEF-specific responses characterized by the changes in the hexose pool that may involve starch and ascorbic acid degradation.
authors.affiliation: Centro de Engenharia Biológica, Institute for Biotechnology and Bioengineering, Universidade do Minho, Campus de Gualtar, 4710-057, Braga, Portugal. Federico.Gomez@food.lth.se
authors.forename: Federico Gómez
authors.initials: FG
authors.lastname: Galindo
authors.forename: Petr
authors.initials: P
authors.lastname: Dejmek
authors.forename: Krister
authors.initials: K
authors.lastname: Lundgren
authors.forename: Allan G
authors.initials: AG
authors.lastname: Rasmusson
authors.forename: António
authors.initials: A
authors.lastname: Vicente
authors.forename: Thomas
authors.initials: T
authors.lastname: Moritz
mesh_terms: D002849:Chromatography, Gas; D002853:Chromatography, Liquid; D016000:Cluster Analysis; D004560:Electricity; D018274:Electroporation; D013058:Mass Spectrometry; D008856:Microscopy, Fluorescence; D011198:Solanum tuberosum / Q000378:metabolism* / Q000502:physiology; D013312:Stress, Physiological