Common sets of promoter elements determine the expression characteristics of three Arabidopsis genes encoding isoforms of mitochondrial cytochrome c oxidase subunit 6b.
The promoters of the three Arabidopsis nuclear genes encoding mitochondrial cytochrome c oxidase subunit 6b (AtCOX6b) have similar expression patterns, with preferential expression in anthers and meristems, and are induced by sucrose and etiolation. Additionally, induction of AtCOX6b-1 by GA(3) and AtCOX6b-3 by 6-benzylaminopurine was observed. Site II elements (TGGGCC/T) present in the three promoters bind common nuclear proteins and are important for basal and induced expression. Induction by sucrose requires, in addition, the integrity of elements with the sequence TACTAA. The results imply the participation of common regulatory factors in the expression of the three Arabidopsis COX6b genes.
Mufarrege
Eduardo F
EF
Instituto de Agrobiotecnología del Litoral (CONICET-UNL), Cátedra de Biología Celular y Molecular, Facultad de Bioquímica y Ciencias Biológicas, Universidad Nacional del Litoral, Santa Fe, Argentina.
Curi
Graciela C
GC
Gonzalez
Daniel H
DH
D017360:Arabidopsis / Q000201:enzymology / Q000235:genetics*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D001593:Benzyl Compounds; D003576:Electron Transport Complex IV / Q000235:genetics / Q000378:metabolism*; D015971:Gene Expression Regulation, Enzymologic; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D017930:Genes, Reporter; D005875:Gibberellins / Q000494:pharmacology; D007701:Kinetin / Q000494:pharmacology; D030821:Plants, Genetically Modified / Q000201:enzymology / Q000235:genetics; D011401:Promoter Regions, Genetic; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism; D011687:Purines; D013395:Sucrose / Q000378:metabolism
D029681:Arabidopsis Proteins; D001593:Benzyl Compounds; D005875:Gibberellins; D020033:Protein Isoforms; D011687:Purines; D013395:Sucrose; C007842:gibberellic acid; D003576:Electron Transport Complex IV; C480551:benzylaminopurine; D007701:Kinetin