Anti-inflammatory effect of Columbianetin on activated human mast cells.
In the present study, we extracted Corydalis heterocarpa with various solvents in order to find the bioactive constituents that demonstrated anti-inflammatory effects. We isolated the active compound, Columbianetin. Anti-inflammatory effect of Columbianetin has been reported but the precise effects of Columbianetin in experimental models have remained unknown. In the present study, we investigate the effect of Columbianetin on the production of histamine, interleukin (IL)-1beta, IL-6, IL-8, and tumor necrosis factor (TNF)-alpha and expression of cyclooxygenase-2 (COX-2) by using the human mast cell line (HMC-1). Various concentrations of Columbianetin were treated before the activation of HMC-1 cells with phorbol 12-myristate 13-acetate (PMA) plus calcium ionophore, A23187. PMA plus A23187 significantly increased IL-1beta, IL-6, IL-8, and TNF-alpha production compared with media control (p<0.05). We also show that the increased cytokines IL-1beta, IL-6, IL-8, and TNF-alpha level was significantly inhibited by Columbianetin in a dose-dependent manner (p<0.05). Maximal inhibition rates of IL-1beta, IL-6, IL-8, and TNF-alpha production by Columbianetin were about 102.6%, 101.1%, 95.8%, and 103.9%, respectively. Columbianetin inhibited expression of COX-2. In addition, the effect of Columbianetin was investigated on the histamine release from HMC-1 stimulated by substance P, which promotes histamine release. Columbianetin also inhibited the histamine release by substance P. In conclusion, these results indicate that Columbianetin may be helpful in regulating mast cell-mediated allergic inflammatory responses.
Jeong
Hyun-Ja
HJ
Biochip Research Center, Hoseo University, Chungnam, Republic of Korea.
Na
Ho-Jeong
HJ
Kim
Su-Jin
SJ
Rim
Hong-Kun
HK
Myung
Noh-Yil
NY
Moon
Phil-Dong
PD
Han
Na-Ra
NR
Seo
Jae-Uk
JU
Kang
Tae-Hee
TH
Kim
Jae-Joong
JJ
Choi
Youngjin
Y
Kang
In-Cheol
IC
Hong
Seung-Heon
SH
Kim
You-Ah
YA
Seo
Young-Wan
YW
Kim
Hyung-Min
HM
Um
Jae-Young
JY
D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000302:isolation & purification / Q000494:pharmacology*; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D031311:Corydalis / Q000737:chemistry; D051546:Cyclooxygenase 2 / Q000096:biosynthesis; D011564:Furocoumarins / Q000302:isolation & purification / Q000494:pharmacology*; D006636:Histamine Release / Q000187:drug effects; D006801:Humans; D007378:Interleukins / Q000096:biosynthesis; D008407:Mast Cells / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis
D000894:Anti-Inflammatory Agents, Non-Steroidal; D011564:Furocoumarins; D007378:Interleukins; D014409:Tumor Necrosis Factor-alpha; C046733:columbianetin; D051546:Cyclooxygenase 2