title: Rapid change of neuropeptide Y levels and gene-expression in the brain of ovariectomized mice after administration of 17beta-estradiol.
abstract: Estrogen alters excitability and changes synaptic morphology in the rat hippocampal formation. We have compared, by means of radioimmunoassay and in situ hybridization, the effects of short-term treatment with 17beta-estradiol on neuropeptide Y (NPY) in the brain of ovariectomized mice. A highly significant reduction in concentrations of NPY-like immunoreactivity (LI) was observed in the hippocampal formation, some cortical areas and the caudate nucleus 1h after administration of 17beta-estradiol as compared to the control group. In contrast, NPY transcript levels increased in the hippocampal formation (dentate gyrus) and the caudate nucleus, possibly representing a compensatory increase of NPY synthesis following increased estradiol-induced NPY release. These data suggest that 17beta-estradiol, via membrane-related mechanisms, increases NPY release and synthesis in forebrain areas involved in cognition, mood and motor functions.
authors.affiliation: Department of Clinical and Experimental Medicine, Division of Clinical Chemistry, Faculty of Health and Sciences, University Hospital, SE-581 85 Linköping, Sweden. sushi@ibk.liu.se
authors.forename: Susanne
authors.initials: S
authors.lastname: Hilke
authors.forename: Lovisa
authors.initials: L
authors.lastname: Holm
authors.forename: Katarina
authors.initials: K
authors.lastname: Man
authors.forename: Tomas
authors.initials: T
authors.lastname: Hökfelt
authors.forename: Elvar
authors.initials: E
authors.lastname: Theodorsson
mesh_terms: D000818:Animals; D001921:Brain / Q000033:anatomy & histology / Q000187:drug effects / Q000378:metabolism*; D004958:Estradiol / Q000097:blood / Q000494:pharmacology*; D005260:Female; D015870:Gene Expression / Q000187:drug effects*; D051379:Mice; D009478:Neuropeptide Y / Q000235:genetics* / Q000378:metabolism*; D010052:Ovariectomy; D011863:Radioimmunoassay; D051381:Rats
chemical_list: D009478:Neuropeptide Y; D004958:Estradiol