Tissue microarray and immunohistochemistry as tools for evaluation of antibodies against Chlamydia-like bacteria.
Tissue microarray technology was used to establish immunohistochemistry protocols and to determine the specificity of new antisera against various Chlamydia-like bacteria for future use on formalin-fixed and paraffin-embedded tissues. The antisera exhibited strong reactivity against autologous antigen and closely related heterologous antigen, but no cross-reactivity with distantly related species.
Borel
Nicole
N
Institute of Veterinary Pathology, Vetsuisse Faculty, University of Zurich, Zurich, Switzerland.
Casson
Nicola
N
Center for Research on Intracellular Bacteria, Institute of Microbiology, University of Lausanne, Lausanne, Switzerland.
Entenza
José M
JM
Department of Fundamental Microbiology, University of Lausanne, Lausanne, Switzerland.
Kaiser
Carmen
C
Institute of Veterinary Pathology, Vetsuisse Faculty, University of Zurich, Zurich, Switzerland.
Pospischil
Andreas
A
Institute of Veterinary Pathology, Vetsuisse Faculty, University of Zurich, Zurich, Switzerland.
Greub
Gilbert
G
Center for Research on Intracellular Bacteria, Institute of Microbiology, University of Lausanne, Lausanne, Switzerland.
D048248:Acanthamoeba castellanii / Q000382:microbiology; D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology*; D000918:Antibody Specificity; D002695:Chlamydiales / Q000276:immunology*; D002522:Chlorocebus aethiops; D003429:Cross Reactions; D007106:Immune Sera / Q000276:immunology; D007150:Immunohistochemistry; D051379:Mice; D046228:Microarray Analysis / Q000379:methods*; D014709:Vero Cells
D000907:Antibodies, Bacterial; D007106:Immune Sera