title: Dependence of swarming in Escherichia coli K-12 on spermidine and the spermidine importer.
abstract: In a previous work, it was observed that the swarming of polyamine-deficient Proteus mirabilis (speB::sm) was severely inhibited on Luria-Bertani (LB) swarming plates (LBSw) (LB, 0.5% glucose, 0.5% agar), and it was clarified that extracellular putrescine was important as a signaling molecule for the induction of swarming in P. mirabilis. However, a polyamine-deficient strain (delta-speAB delta-speC) of Escherichia coli swarmed as well as the parental strain on LBSw plates. We report that the swarming phenotype of a polyamine-deficient E. coli strain is dependent on spermidine and PotABCD, a spermidine importer.
authors.affiliation: Japan Collection of Microorganisms, Microbe Division, RIKEN BioResource Center, Wako, Saitama, Japan. kuri711@jcm.riken.jp
authors.forename: Shin
authors.initials: S
authors.lastname: Kurihara
authors.forename: Hideyuki
authors.initials: H
authors.lastname: Suzuki
authors.forename: Yuichi
authors.initials: Y
authors.lastname: Tsuboi
authors.forename: Yoshimi
authors.initials: Y
authors.lastname: Benno
mesh_terms: D018528:ATP-Binding Cassette Transporters / Q000235:genetics / Q000378:metabolism*; D048168:Escherichia coli K12 / Q000502:physiology*; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism*; D017353:Gene Deletion; D008124:Locomotion; D026901:Membrane Transport Proteins / Q000235:genetics / Q000378:metabolism*; D033902:Periplasmic Binding Proteins / Q000235:genetics / Q000378:metabolism*; D013095:Spermidine / Q000378:metabolism*
chemical_list: D018528:ATP-Binding Cassette Transporters; D029968:Escherichia coli Proteins; D026901:Membrane Transport Proteins; D033902:Periplasmic Binding Proteins; C096374:PotA protein, E coli; C096375:PotB protein, E coli; C096376:PotC protein, E coli; C099274:PotD protein, E coli; D013095:Spermidine