title: Microfluidic chip for combinatorial mixing and screening of assays.
abstract: This paper reports the design, fabrication and validation of a microfluidic well plate for combinatorial screening applications. Each well within the array is comprised of two 200 picoliter compartments that each contain a photonic crystal biosensor to enable the on-chip, in situ detection of (bio-) molecular binding events. This microfluidic chip utilizes arrays of Actuate-to-Open valves to isolate all compartments, which allows the chip to be decoupled from pneumatic control lines and thus to be transported freely between filling, sensing and characterization platforms. A proof-of-principle 4 x 4 protein/antibody binding assay was performed to demonstrate the discrete mixing and on-chip sensing capabilities.
authors.affiliation: University of Illinois at Urbana-Champaign, Chemical & Biomolecular Engineering, Urbana, IL 61801, USA.
authors.forename: Benjamin R
authors.initials: BR
authors.lastname: Schudel
authors.forename: Charles J
authors.initials: CJ
authors.lastname: Choi
authors.forename: Brian T
authors.initials: BT
authors.lastname: Cunningham
authors.forename: Paul J A
authors.initials: PJ
authors.lastname: Kenis
mesh_terms: D000818:Animals; D000906:Antibodies / Q000378:metabolism; D015374:Biosensing Techniques / Q000295:instrumentation*; D004867:Equipment Design; D006801:Humans; D046210:Microfluidic Analytical Techniques / Q000379:methods*; D055616:Microtechnology; D055095:Optics and Photonics
chemical_list: D000906:Antibodies