Cobalt chloride-induced downregulation of puromycin-sensitive aminopeptidase suppresses the migration and invasion of PC-3 cells.
Cobalt chloride (CoCl(2)) treatment of cells in vitro has been shown to induce cellular changes that are similar to those seen following hypoxia. To identify genes that are differentially expressed in response to treatment with CoCl(2), we compared the mRNA expression profiles of PC-3 cells that were treated with CoCl(2) with those of untreated PC-3 cells, using specific arbitrary primers and two anchored oligo(dT) primers provided in the ACP-based GeneFishing kits. The results of this study demonstrated that the puromycin-sensitive aminopeptidase (PSA) gene was downregulated in PC-3 cells that were treated with CoCl(2). This downregulation of PSA expression, in turn, suppressed the proliferation, migration, and invasion of PC-3 cells, as well as the secretion and expression of matrix metalloproteinase-9 (MMP-9).
Lee
Suk Hee
SH
Division of Biological Sciences, Research Center of Bioactive Materials, Chonbuk National University, Chonju 561-756, Korea.
Kim
Hwan Gyu
HG
D000626:Aminopeptidases / Q000235:genetics / Q000378:metabolism*; D016587:Antimutagenic Agents / Q000494:pharmacology; D015687:Cell Hypoxia / Q000235:genetics; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects; D003035:Cobalt / Q000494:pharmacology*; D015536:Down-Regulation / Q000187:drug effects*; D015870:Gene Expression / Q000187:drug effects; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D020780:Matrix Metalloproteinase 9 / Q000096:biosynthesis; D009389:Neovascularization, Pathologic / Q000235:genetics / Q000378:metabolism
D016587:Antimutagenic Agents; D003035:Cobalt; D000626:Aminopeptidases; C025579:enkephalin degrading enzyme; D020780:Matrix Metalloproteinase 9; C018021:cobaltous chloride