title: Genic expression of the uterine leiomyoma in reproductive-aged women after treatment with goserelin.
abstract: OBJECTIVE
  To identify the genes presenting different expression in uterine leiomyomas after goserelin treatment.
  
  
  METHODS
  Retrospective analyses of tissue obtained in a prospective clinical study.
  
  
  METHODS
  School of Medicine of the University of São Paulo.
  
  
  METHODS
  30 nulliparous black women aged 20 to 45 years with symptoms of uterine leiomyoma, uterine volume over 300 mL, and surgical indications for myomectomy.
  
  
  METHODS
  Fifteen patients were given a monthly dose of 3.6 mg of goserelin over 3 months before surgery (group A), and 15 patients underwent surgery without any previous treatment (group B). Five random samples from each group were analyzed using the microarray technique with the Affymetrix platform (GeneChip Rat Genome 230 2.0 Array).
  
  
  METHODS
  Quantification of transcript expression levels of uterine fibroids in patients treated or not treated with goserelin.
  
  
  RESULTS
  Of the total of 47,000 sequences that were analyzed, representing approximately 38,500 human genes already characterized, we found a differential expression of 174 genes. Of these, 70 were up-regulated (33 genes with known function) and 104 were down-regulated (65 genes with known function) in samples from group A (treated) when compared with group B (nontreated).
  
  
  CONCLUSIONS
  The genic expression of uterine leiomyomas changes in women who have had goserelin treatment when compared with nontreated patients.
authors.affiliation: LIM-58 of the Division of Gynecology, Department of Obstetrics and Gynecology, School of Medicine, Faculdade de Medicina da Universidade de São Paulo (FMUSP), São Paulo, Brazil. roborsari@hotmail.com
authors.forename: Rodrigo
authors.initials: R
authors.lastname: Borsari
authors.forename: Nilo
authors.initials: N
authors.lastname: Bozzini
authors.forename: Consuelo Rodrigues
authors.initials: CR
authors.lastname: Junqueira
authors.forename: José Maria
authors.initials: JM
authors.lastname: Soares
authors.forename: Sandro Garcia
authors.initials: SG
authors.lastname: Hilário
authors.forename: Edmund C
authors.initials: EC
authors.lastname: Baracat
mesh_terms: D000328:Adult; D000367:Age Factors; D018931:Antineoplastic Agents, Hormonal / Q000494:pharmacology / Q000627:therapeutic use; D005260:Female; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects*; D017273:Goserelin / Q000494:pharmacology* / Q000627:therapeutic use; D006801:Humans; D007889:Leiomyoma / Q000188:drug therapy / Q000235:genetics* / Q000473:pathology; D008875:Middle Aged; D012098:Reproduction / Q000502:physiology; D012189:Retrospective Studies; D046888:Tissue Array Analysis; D014594:Uterine Neoplasms / Q000188:drug therapy / Q000235:genetics* / Q000473:pathology; D055815:Young Adult
chemical_list: D018931:Antineoplastic Agents, Hormonal; D017273:Goserelin