Expression or secretion of IL-17 in the peripheral blood mononuclear cells from patients with dilated cardiomyopathy.
OBJECTIVE
The objective of the study was to investigate the relation between interleukin-17 (IL-17) level in the plasma and in peripheral blood mononuclear cells (PBMCs), and dilated cardiomyopathy (DCM) disease severity.


RESULTS
30 patients with DCM and 20 normal adults as control were studied. IL-17 protein level in plasma, PBMC culture supernatants, and phytohaemagglutinin-stimulated PBMC culture supernatants were measured with ELISA. IL-17 mRNA expression in PBMCs was detected by reverse transcription-polymerase chain reaction. Results showed that the IL-17 protein level in PHA-stimulated PBMC culture supernatants or its mRNA level in the PHA-stimulated PBMC, but not in plasma or in PBMC culture supernatants, was significantly elevated in DCM patients compared with normal control subjects.The IL-17 protein level in cultured supernatants and the IL-17 mRNA level in the PBMCs were strongly correlated with the heart function of DCM patients.


CONCLUSIONS
The ability to express IL-17 protein or mRNA in PBMC is abnormal and the change strongly correlates with the heart function of DCM patients.
Huang
Yanlan
Y
Dept. of Cardiology, the First Affiliated Hospital of Guangxi Medical University, Nanning, People's Republic of China.
Wu
Weifeng
W
Wang
Yan
Y
D015415:Biomarkers / Q000097:blood*; D002311:Cardiomyopathy, Dilated / Q000097:blood* / Q000235:genetics; D002478:Cells, Cultured; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005786:Gene Expression Regulation; D020022:Genetic Predisposition to Disease; D006801:Humans; D020381:Interleukin-17 / Q000096:biosynthesis* / Q000235:genetics*; D007963:Leukocytes, Mononuclear / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D011379:Prognosis; D012333:RNA, Messenger / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction
D015415:Biomarkers; D020381:Interleukin-17; D012333:RNA, Messenger