title: Glucosinolate engineering identifies a gamma-glutamyl peptidase.
abstract: Consumption of cruciferous vegetables is associated with reduced risk of developing cancer, a phenomenon attributed to glucosinolates, which are characteristic of these vegetables. We report production of the bioactive benzylglucosinolate in the noncruciferous plant Nicotiana benthamiana through metabolic engineering. The study includes identification of gamma-glutamyl peptidase 1 (GGP1), which substantially increased glucosinolate production by metabolizing an accumulating glutathione conjugate, an activity not previously described for glucosinolate biosynthesis or for proteins containing glutamine amidotransferase domains.
authors.affiliation: Plant Biochemistry Laboratory, Department of Plant Biology, Faculty of Life Sciences, University of Copenhagen, Frederiksberg, Denmark.
authors.forename: Fernando
authors.initials: F
authors.lastname: Geu-Flores
authors.forename: Morten Thrane
authors.initials: MT
authors.lastname: Nielsen
authors.forename: Majse
authors.initials: M
authors.lastname: Nafisi
authors.forename: Morten Emil
authors.initials: ME
authors.lastname: Møldrup
authors.forename: Carl Erik
authors.initials: CE
authors.lastname: Olsen
authors.forename: Mohammed Saddik
authors.initials: MS
authors.lastname: Motawia
authors.forename: Barbara Ann
authors.initials: BA
authors.lastname: Halkier
mesh_terms: D005961:Glucosinolates / Q000096:biosynthesis*; D016366:Open Reading Frames; D018515:Plant Leaves / Q000201:enzymology / Q000235:genetics / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics*; D030821:Plants, Genetically Modified / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism; D013861:Thiocyanates; D013863:Thioglucosides / Q000096:biosynthesis*; D014026:Nicotiana / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism; D014170:Transformation, Genetic
chemical_list: D005961:Glucosinolates; D010940:Plant Proteins; D013861:Thiocyanates; D013863:Thioglucosides; C408882:benzylglucosinolic acid