Development of a radioimmunoassay for the measurement of Bisphenol A in biological samples.
Bisphenol A (BPA) is widely used in the manufacturing of polycarbonate plastic food and drink packaging. Possessing a weak estrogenic activity, BPA is listed among a growing list of endocrine disrupting compounds. In this study, a polyclonal anti-BPA antibody was obtained by immunization with BPA-monocarboxymethylether covalently linked to BSA. The antibody demonstrates negligible cross-reactivity with most analogous BPA phenolic structures, and no cross-reactivity with endogenous steroids. An extraction step with ethyl acetate minimized matrix effects and allowed the BPA measurement in plasma and other biological samples. Recovery after loading test was 96 +/- 4% and dilution tests had a linear profile (r2 > 0.93). The limit of detection of the BPA RIA was 0.08 microg L(-1) with an IC50 of 1.25 microg L(-1). The intra- and inter-assay coefficients of variation were 5.6 and 8.6%, respectively at a BPA concentration of 0.7 microg L(-1) and 6.9 and 5.7% at a BPA concentration of 1.3 microg L(-1). A significant correlation was found between the values obtained by the RIA and HPLC-MS (r2 = 0.92) or HPLC coupled to a fluorescence detector (r2 = 0.80). In conclusion, we described a BPA-RIA that is a suitable tool for evaluating human exposure to BPA.
Kaddar
Nisrin
N
Université de Lyon 1 ISBP, France.
Bendridi
Nadia
N
Harthé
Catherine
C
de Ravel
Marc Rolland
MR
Bienvenu
Anne-Lise
AL
Cuilleron
Claude-Yves
CY
Mappus
Elisabeth
E
Pugeat
Michel
M
Déchaud
Henri
H
D000818:Animals; D000906:Antibodies / Q000276:immunology; D001559:Benzhydryl Compounds; D002417:Cattle; D003429:Cross Reactions; D005260:Female; D015571:Follicular Fluid / Q000737:chemistry; D006801:Humans; D008297:Male; D010636:Phenols / Q000032:analysis* / Q000097:blood / Q000276:immunology*; D011863:Radioimmunoassay / Q000191:economics / Q000379:methods*; D018401:Sample Size; D012661:Semen / Q000737:chemistry; D012680:Sensitivity and Specificity; D000098826:Bisphenol A Compounds
D000906:Antibodies; D001559:Benzhydryl Compounds; D010636:Phenols; C006780:bisphenol A; D000098826:Bisphenol A Compounds