RANTES In1.1C allele polymorphisms in 13 Chinese ethnic populations.
BACKGROUND
The In1.1C single nucleotide polymorphism (SNP) allele results in reduced RANTES transcription, which is associated with increased frequency of HIV-1 infection, and rapid progression to AIDS among HIV-1-infected individuals. This study aimed to study the mutant frequency and polymorphism of RANTES in Chinese populations.


METHODS
The genotypes of RANTES In1.1C were determined by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) with the digestion of restriction endonuclease Mbo II.


RESULTS
Of the 617 individuals, 290 (47%) were carriers of the RANTES In1.1C allele, 52 of whom were homozygotes, whereas 238 were heterozygotes. The frequency of the RANTES In1.1C allele in those tested individuals was 0.2840. The frequencies of In1.1C allele varied from 0.07 - 0.27 in most of the populations in South-west China except for the two Lisu populations, while the frequencies of In1.1C spans from 0.35 to 0.45 in North-west China. The prevalence of the allele varied substantially between the South-west groups and North-west groups (chi(2) = 7.838, P = 0.006).


CONCLUSIONS
The prevalence of the RANTES In1.1C allele varies substantially between the South-west groups and North-west groups. There is no significant difference between the groups with different languages, which suggests that language relationship is not consistent with the genetic relationship. These results have important implications for the design, assessment, and implementation of HIV-1 vaccines.
Qian
Yuan
Y
Institute of Medical Biology, Chinese Academy of Medical Science & Peking Union Medical College, Kunming 650118, China.
Sun
Hao
H
Chu
Jia-you
JY
D000483:Alleles; D044466:Asian People / Q000235:genetics; D018946:Chemokine CCL5 / Q000235:genetics*; D005006:Ethnicity / Q000235:genetics; D020022:Genetic Predisposition to Disease / Q000235:genetics; D005838:Genotype; D006801:Humans; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length / Q000235:genetics; D020641:Polymorphism, Single Nucleotide / Q000235:genetics; D015995:Prevalence
D018946:Chemokine CCL5