title: [In vitro analysis of intake of minocycline by mature rat mandibular osteoblasts].
abstract: OBJECTIVE
  To observe the intake of minocycline and its amount in mature rat mandibular osteoblasts (MRMOB) in vitro, and to identify the feasibility of intracellular anti-bacterial activity of minocycline.
  
  
  METHODS
  Four groups of MRMOB were incubated in 100 mg/L minocycline for 15, 30, 45 and 60 minutes respectively, and the accumulation of minocycline within MRMOB was measured using a fluorescence spectrophotometer.
  
  
  RESULTS
  The intracellular accumulation amount of minocycline in the four groups of MRMOB was (17.29 +/- 1.49), (16.87 +/- 1.57), (16.96 +/- 1.67) and (17.94 +/- 1.63) mg/g respectively after osteoblasts were cultured in Dulbecco's modified Eagle's medium (DMEM) which contained minocycline for 15, 30, 45 and 60 min. There was no significant difference in amount of minocycline among the four groups of MRMOB.
  
  
  CONCLUSIONS
  The mature rat mandibular osteoblasts can ingest minocycline, and the accumulation amount of minocycline in MRMOB is irrelevant with the exposure time of MRMOB to minocycline.
authors.affiliation: Stomatological Institute, General Hospital of PLA, Beijing 100853, China.
authors.forename: Wen-Yi
authors.initials: WY
authors.lastname: Zhang
authors.forename: Hong-Chen
authors.initials: HC
authors.lastname: Liu
authors.forename: Xia
authors.initials: X
authors.lastname: Wu
authors.forename: Ling-Ling
authors.initials: LL
authors.lastname: E
authors.forename: Yan
authors.initials: Y
authors.lastname: Lü
authors.forename: Jian
authors.initials: J
authors.lastname: Zhang
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D008334:Mandible / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D008911:Minocycline / Q000493:pharmacokinetics*; D010006:Osteoblasts / Q000187:drug effects* / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D008911:Minocycline