Drug modulation of transductor function of Na+,K+-ATPase.
Experimental proof of the hypothesis on modulation of the transductor function of Na+,K+-ATPase by the ouabain-Ca2+ chelate complex was obtained by the method organotypic tissue culture. Quantum chemical estimations detected two principally different modes of Ca2+ ion chelation by ouabain molecule. It is hypothesized that the ouabain-Ca2+-Na+,K+-ATPase ligand-receptor complex is formed due to ion-ion bonds. The formation of the complex serves as the signal triggering the enzyme transductor function. It is experimentally proven that ouabain is incapable of inhibiting neurite growth in sensory neuron and heart tissue explants after removal of free calcium from the nutrient medium with EGTA.
Lopatina
E V
EV
Department of Experimental Pharmacology, V. A. Almazov Federal Center of the Heart, Blood, and Endocrinology, Federal Agency for Medical Technologies, St. Petersburg, Russia. evl112@infran.ru
Pennyaynen
V A
VA
Rogachevskyi
I V
IV
Krylov
B V
BV
D000818:Animals; D002118:Calcium / Q000737:chemistry; D002642:Chick Embryo; D002645:Chickens; D004789:Enzyme Activation / Q000187:drug effects; D006321:Heart / Q000187:drug effects; D015394:Molecular Structure; D016501:Neurites / Q000187:drug effects; D010042:Ouabain / Q000737:chemistry / Q000494:pharmacology*; D011984:Sensory Receptor Cells / Q000187:drug effects; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism*; D046509:Tissue Culture Techniques
D010042:Ouabain; D000254:Sodium-Potassium-Exchanging ATPase; D002118:Calcium