Ion chromatography tandem mass spectrometry for simultaneous confirmation and determination of indandione rodenticides in serum.
This paper describes a simple method for the simultaneous determination and confirmation of the indandione rodenticides in serum. After samples were extracted with 10% (v/v) methanol in acetonitrile and cleaned by solid-phase extraction, chromatographic separation was performed on an IonPac AS11 analytical column (250 x 4.0 mm) using gradient KOH eluent with 10% (v/v) methanol as organic modifier. Confirmation was depended on the extensive fragmentation of the indandione molecule under MS/MS conditions which provides sufficient structural information. Quantification was performed by negative electrospray ionization in multiple reaction monitoring mode. All the method parameters were validated. It was confirmed that this method could be used in clinical diagnosis and forensic toxicology.
Chen
Xiao-Hong
XH
Ningbo Key Laboratory of Poison Research and Control, Ningbo Municipal Center for Disease Control and Prevention, Ningbo 315010, China.
Cai
Mei-Qiang
MQ
Ouyang
Xiao-Kun
XK
Jin
Mi-Cong
MC
D002852:Chromatography, Ion Exchange; D006801:Humans; D007189:Indans / Q000097:blood*; D000432:Methanol / Q000737:chemistry; D011189:Potassium Chloride / Q000737:chemistry; D015203:Reproducibility of Results; D012378:Rodenticides / Q000097:blood*; D012680:Sensitivity and Specificity; D052616:Solid Phase Extraction; D053719:Tandem Mass Spectrometry
D007189:Indans; D012378:Rodenticides; D011189:Potassium Chloride; D000432:Methanol