title: Comparison of MRI signatures in pattern I and II multiple sclerosis models.
abstract: The majority of individuals with multiple sclerosis (MS) exhibit T-cell- and macrophage-dominated lesions (patterns I and II; as opposed to III and IV). These lesions, in turn, may be distinguished on the basis of whether or not there are immunoglobulin and complement depositions at the sites of active myelin destruction; such depositions are found exclusively in pattern II lesions. The main aim of this study was to determine whether pattern I and pattern II MS lesions exhibit distinct MRI signatures. We have used a recently described focal MOG-induced EAE model of the rat brain, which recapitulates many of the hallmarks of pattern II MS; we compared this with our previous work in a delayed type hypersensitivity model of a pattern I type lesion in the rat brain. Demyelinating lesions with extensive inflammation were generated, in which the T2-weighted signal was increased. Magnetisation transfer ratio (MTR) maps revealed loss and subsequent incomplete recovery of the structure of the corpus callosum, together with changes in tissue water diffusion and an associated increase in ventricle size. Notably, the MTR changes preceeded histological demyelination and may report on the processes leading to demyelination, rather than demyelination per se. Immunohistochemically, these MRI-detectable signal changes correlated with both inflammatory cell infiltration and later loss of myelin. Breakdown of the blood-brain barrier and an increase in the regional cerebral blood volume were also evident in and around the lesion site at the early stage of the disease. Interestingly, however, the MRI signal changes in this pattern II type MS lesion were remarkably consistent with those previously observed in a pattern I lesion. These findings suggest that the observed signal changes reflect the convergent histopathology of the two models rather than the underlying mechanisms of the disease.
authors.affiliation: Department of Physiology, Anatomy & Genetics, University of Oxford, Oxford, UK. sebastien.serres@rob.ox.ac.uk
authors.forename: Sébastien
authors.initials: S
authors.lastname: Serres
authors.forename: Daniel C
authors.initials: DC
authors.lastname: Anthony
authors.forename: Yanyan
authors.initials: Y
authors.lastname: Jiang
authors.forename: Sandra J
authors.initials: SJ
authors.lastname: Campbell
authors.forename: Kerry A
authors.initials: KA
authors.lastname: Broom
authors.forename: Alexandre
authors.initials: A
authors.lastname: Khrapitchev
authors.forename: Nicola R
authors.initials: NR
authors.lastname: Sibson
mesh_terms: D000818:Animals; D001812:Blood-Brain Barrier / Q000378:metabolism / Q000473:pathology; D001921:Brain / Q000033:anatomy & histology / Q000473:pathology*; D002560:Cerebrovascular Circulation; D004195:Disease Models, Animal; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000473:pathology*; D006801:Humans; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D008279:Magnetic Resonance Imaging / Q000379:methods*; D008297:Male; D009103:Multiple Sclerosis / Q000473:pathology*; D051381:Rats; D011917:Rats, Inbred Lew; D012039:Regional Blood Flow; D014867:Water / Q000378:metabolism
chemical_list: D014867:Water