title: Synthesis and characterization of chitosan-silicate hydrogel as resorbable vehicle for bonelike-bone graft.
abstract: The use of bone grafts is required to restore skeletal integrity and enhance bone healing of large defects in several areas of regenerative medicine, such as: orthopedic and maxillofacial procedures. Some of these bone grafts can be resorbed in a time controlled way, in order to allow the correct process of natural re-construction of the involved bone tissue to occur. The Bonelike graft is a bone substitute that mimics the inorganic composition of bone; this biomaterial was developed and characterized over the last decade. In a granular form, Bonelike has proved its highly bioactive behavior in medical applications, such as; maxillofacial and orthopedics surgery. The clinical applications in maxillary bone defects indicated a good bone bonding between new formed bone and the Bonelike granules. The purpose of this study was to develop a new injectable system for the application of Bonelike using a resorbable vehicle which may be used in minimal invasive surgery. A new hydrogel derived from chitosan and y-glycidoxypropyltrimethoxysilane (GPTMS) was synthesized and characterized. The mixture derived from chitosan and GPTMS existed in sol state at room temperature and formed a hydrogel at 37 degrees C. The degradability of the hydrogel could be controlled by the concentration of chitosan and GPTMS, and the presence the presence of Bonelike did not affect its degradability. The pH changes caused by the degradation of this hydrogel were small, so it is not expected to cause any deleterious effect in vivo conditions.
authors.affiliation: Faculdade de Engenharia da Universidade do Porto, Seccáo de Materiais, DEMM, Rua Dr Roberto Frias, 4200-465, Porto, Portugal.
authors.forename: Yuki
authors.initials: Y
authors.lastname: Shirosaki
authors.forename: Cláudia M
authors.initials: CM
authors.lastname: Botelho
authors.forename: Maria A
authors.initials: MA
authors.lastname: Lopes
authors.forename: José D
authors.initials: JD
authors.lastname: Santos
mesh_terms: D001672:Biocompatible Materials; D001842:Bone and Bones; D048271:Chitosan / Q000737:chemistry*; D020100:Hydrogels / Q000138:chemical synthesis* / Q000737:chemistry; D008855:Microscopy, Electron, Scanning; D019736:Prostheses and Implants; D017640:Silicates / Q000737:chemistry*; D013057:Spectrum Analysis
chemical_list: D001672:Biocompatible Materials; D020100:Hydrogels; D017640:Silicates; D048271:Chitosan