title: Correlation of p53, MDM2 and p14(ARF) protein expression in human esophageal squamous cell carcinoma.
abstract: OBJECTIVE
  To determine the interrelationships of p53, MDM2, and p14(ARF) protein expression in primary esophageal squamous cell carcinoma (ESCC) and their prognostic value in ESCC.
  
  
  METHODS
  In total, 119 patients treated for ESCC with esophagectomy were enrolled in this study. Demographic and clinical data including gender, age, depth of tumor invasion, lymph node involvement, and 5-year survival rate were collected by chart review. p53, MDM2, and p14(ARF) were detected immunohistochemically in the resected tumors to evaluate their usefulness as biomarkers of clinical outcome.
  
  
  RESULTS
  p53, MDM2, and p14(ARF) were expressed in 61 (51.3%), 34 (28.6%), and 22 (18.5%) of 119 tumor specimens, respectively. Overall, p53 protein expression was positively correlated with MDM2 (P = 0.024) and p14(ARF) expression (P = 0.026). In addition, p14(ARF) expression was most often found in specimens that were positive for both p53 and MDM2. Changes in the p53, MDM2, and p14(ARF) protein levels were not correlated with 5-year survival rate.
  
  
  CONCLUSIONS
  Expression of p53 protein correlates with increased MDM2 and p14(ARF) protein levels in ESCC. In addition, status of p53 (wild-type versus mutant) rather than expression level of p53, MDM2, or p14(ARF) is likely to be the more critical determinant of clinical outcome.
authors.affiliation: Institute of Biochemistry and Molecular Biology, National Yang-Ming University, Taipei, Taiwan, ROC.
authors.forename: Tzu-Hao
authors.initials: TH
authors.lastname: Cheng
authors.forename: Po-Kuei
authors.initials: PK
authors.lastname: Hsu
authors.forename: Anna Fen-Yau
authors.initials: AF
authors.lastname: Li
authors.forename: I-Chun
authors.initials: IC
authors.lastname: Hung
authors.forename: Min-Hsiung
authors.initials: MH
authors.lastname: Huang
authors.forename: Han-Shui
authors.initials: HS
authors.lastname: Hsu
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002294:Carcinoma, Squamous Cell / Q000737:chemistry* / Q000401:mortality / Q000473:pathology; D004938:Esophageal Neoplasms / Q000737:chemistry* / Q000401:mortality / Q000473:pathology; D005260:Female; D016158:Genes, p53; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D051736:Proto-Oncogene Proteins c-mdm2 / Q000032:analysis* / Q000502:physiology; D025803:Tumor Suppressor Protein p14ARF / Q000032:analysis* / Q000502:physiology; D016159:Tumor Suppressor Protein p53 / Q000032:analysis* / Q000502:physiology
chemical_list: D025803:Tumor Suppressor Protein p14ARF; D016159:Tumor Suppressor Protein p53; C497206:MDM2 protein, human; D051736:Proto-Oncogene Proteins c-mdm2