Degradation of rutin by Thermoactinomyces vulgaris and other thermophilic compost isolates.
The mutagenic effects of rutin and quercetin have aroused the interest of many investigators. To develop the microbial degradation of rutin, a thermophilic actinomycete, which could hydrolyze rutin, was isolated from compost soil. The taxonomical characteristics of this thermophilic actinomycete were examined and identified as Thermoactinomyces vulgaris PU18-2. After cultivation of T. vulgaris PU18-2 in the rutin-CYC medium for 60 h, the culture filtrate had a rutin-degrading ability, but the cell-free extract did not. There was no quercetin, rutinose, rhamnose, and glucose accumulated in the rutin hydrolysate of the culture filtrate. Both alpha-rhamnosidase and beta-glucosidase activities were not found in the culture filtrate of the T. vulgaris PU18-2 in the rutin-CYC medium. These results showed that the initial attack on rutin by the extracellular enzymes of T. vulgaris PU18-2 apparently was not through the glycosidase-mediated hydrolysis of glycosidic bond.
Yang
Chao-Hsun
CH
Department of Cosmetic Science, Providence University, Shalu, Taichung, Taiwan. chyang@pu.edu.tw
Huang
Yu-Chun
YC
Chen
Cheng-Yu
CY
D001426:Bacterial Proteins / Q000378:metabolism; D006026:Glycoside Hydrolases / Q000378:metabolism; D006868:Hydrolysis; D008848:Micromonosporaceae / Q000201:enzymology / Q000302:isolation & purification / Q000378:metabolism*; D012431:Rutin / Q000378:metabolism*; D012987:Soil / Q000032:analysis*; D012988:Soil Microbiology; D001617:beta-Glucosidase / Q000378:metabolism
D001426:Bacterial Proteins; D012987:Soil; D012431:Rutin; D006026:Glycoside Hydrolases; D001617:beta-Glucosidase; C022525:alpha-L-rhamnosidase