Protective effects of mutant of acidic fibroblast growth factor against cerebral ischaemia-reperfusion injury in rats.
OBJECTIVE
To investigate the protective effect of a mutant of acidic fibroblast growth factor (MaFGF) against cerebral ischaemia-reperfusion injury in rats.


METHODS
Sixty male Sprague-Dawley rats were randomly divided into six groups as follows: sham-operated group, untreated group, 20microg/kg, 40microg/kg and 80microg/kg MaFGF-treated groups and also the positive control group. Cerebral ischaemia-reperfusion injury was induced by middle cerebral artery occlusion (MCAO) for 2h followed by reperfusion for 24h. Different dose of MaFGF were infused intravenously at 1h after middle cerebral artery (MCA) occlusion. Nimodipine was used as positive control. The behaviour deficit score, brain-infarcted area, brain oedema degree, malondialdehyde (MDA) content and superoxide dismutase (SOD) activity were detected at 24h after reperfusion.


RESULTS
The results showed that MaFGF at the dose of 20microg/kg, 40microg/kg and 80microg/kg significantly alleviated brain injury. Compared to untreated group, the behaviour deficits were much less severe, the brain oedema alleviated obviously, the MDA contents decreased and SOD activity increased dramatically in MaFGF-treated groups respectively. The efficacy of MaFGF was similar to that of nimodipine.


CONCLUSIONS
The results demonstrate that MaFGF has neuroprotective effect against brain injury resulting from focal ischaemia-reperfusion in Sprague-Dawley rats.
Xu
Hua
H
Pharmacy College of Jinan University, Guangzhou, PR China. huax-mail@163.com
Li
Xiao-kun
XK
Zheng
Qing
Q
Huang
Ya-dong
YD
Yao
Cheng-can
CC
Su
Zhi-jian
ZJ
Zhao
Wen
W
Zhao
Zhuan-you
ZY
D000818:Animals; D001929:Brain Edema / Q000517:prevention & control; D002545:Brain Ischemia / Q000517:prevention & control*; D002544:Cerebral Infarction / Q000378:metabolism / Q000517:prevention & control; D004305:Dose-Response Relationship, Drug; D016220:Fibroblast Growth Factor 1 / Q000008:administration & dosage*; D020244:Infarction, Middle Cerebral Artery / Q000378:metabolism / Q000517:prevention & control*; D008026:Ligation; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D020768:Middle Cerebral Artery; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000378:metabolism / Q000517:prevention & control*; D013482:Superoxide Dismutase / Q000378:metabolism
D016220:Fibroblast Growth Factor 1; D008315:Malondialdehyde; D013482:Superoxide Dismutase