title: Comparative genomic hybridization on spotted oligonucleotide microarrays.
abstract: Recent advances in DNA microarray technology have enabled researchers to comprehensively characterize the complex genomes of higher eukaryotic organisms at an unprecedented level of detail. Array-based comparative genomic hybridization (Array-CGH) has been widely used for detecting DNA copy number alterations on a genomic scale, where the mapping resolution is limited only by the number of probes on the DNA microarray. In this chapter, we present a validated protocol utilizing print-tip spotted HEEBO (Human Exonic Evidence Based Oligonucleotide) microarrays for conducting array-CGH using as little as 25 ng of genomic DNA from a wide variety of sources, including cultured cell lines and clinical specimens, with high spatial resolution and array-to-array reproducibility.
authors.affiliation: Department of Pathology, Stanford University, Stanford, CA, USA.
authors.forename: Young H
authors.initials: YH
authors.lastname: Kim
authors.forename: Jonathan R
authors.initials: JR
authors.lastname: Pollack
mesh_terms: D055028:Comparative Genomic Hybridization / Q000295:instrumentation / Q000379:methods; D016678:Genome; D006801:Humans; D007091:Image Processing, Computer-Assisted / Q000379:methods; D020411:Oligonucleotide Array Sequence Analysis / Q000295:instrumentation / Q000379:methods; D015203:Reproducibility of Results