Gastroprotective activity of isopulegol on experimentally induced gastric lesions in mice: investigation of possible mechanisms of action.
The present study investigated whether isopulegol, a monoterpene present in essential oils of several aromatic plants, would be able to promote some gastroprotective effect and also verified the possible mechanisms involved in this action. For this study, ethanol- and indomethacin-induced gastric ulcer models in mice and histopathological assessment were used. The roles of NO, sulfhydryls (glutathione, GSH), ATP-sensitive K(+) channels (K(ATP) channels), and prostaglandins were also investigated. Isopulegol exhibited a dose-related gastroprotective effect against ethanol-induced lesions, while the pretreatment with glibenclamide and indomethacin [but not with N(G)-nitro-L-arginine methyl ester] were able to reverse this action. The pretreatment with isopulegol also restored GSH levels to normal levels and exhibited dose-related gastroprotective effect against indomethacin-induced ulcer. The results suggested that isopulegol presents significant gastroprotective effects in both ethanol- and indomethacin-induced ulcer models, which appear to be mediated, at least in part, by endogenous prostaglandins, K(ATP) channel opening, and antioxidant properties.
Silva
Maria Izabel Gomes
MI
Department of Physiology and Pharmacology, Faculty of Medicine, Federal University of Ceará, Rua Cel. Nunes de Melo 1127, Fortaleza, Brazil.
Moura
Brinell Arcanjo
BA
Neto
Manuel Rufino de Aquino
MR
Tomé
Adriana da Rocha
Ada R
Rocha
Nayrton Flávio Moura
NF
de Carvalho
Alyne Mara Rodrigues
AM
Macêdo
Danielle Silveira
DS
Vasconcelos
Silvânia Maria Mendes
SM
de Sousa
Damião Pergentino
DP
Viana
Glauce Socorro de Barros
GS
de Sousa
Francisca Cléa Florenço
FC
D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage / Q000494:pharmacology*; D000081005:Cyclohexane Monoterpenes; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D000431:Ethanol / Q000633:toxicity; D005978:Glutathione / Q000187:drug effects / Q000378:metabolism; D007213:Indomethacin / Q000633:toxicity; D054086:KATP Channels / Q000378:metabolism; D008297:Male; D051379:Mice; D011453:Prostaglandins / Q000378:metabolism; D013276:Stomach Ulcer / Q000139:chemically induced / Q000517:prevention & control*; D013729:Terpenes / Q000008:administration & dosage / Q000494:pharmacology*
D000975:Antioxidants; D000081005:Cyclohexane Monoterpenes; D054086:KATP Channels; D011453:Prostaglandins; D013729:Terpenes; D000431:Ethanol; C409816:isopulegol; D005978:Glutathione; D007213:Indomethacin