Hepatoprotective constituents from Cleome droserifolia.
The effect of ethanol extract from aerial parts of Cleome droserifolia was investigated against carbon tetrachloride induced liver injury. The hepatoprotective activity was evaluated through the quantification of biochemical parameters and confirmed using histopathology analysis. Efficient hepatoprotective effect was achieved by crude extract, fractions and some pure compounds. The phytochemical studies showed that the petroleum ether fraction afforded two known guaiane sesquiterpenes buchariol (1) and teucladiol (2) in addition to daucosterol (beta-sitosterol glucoside) (3). The CHCl(3) fraction afforded three known flavonoid derivatives; 5,3'-dihydroxy-3,6,7,4',5'-pentamethoxyflavone (4), 5'-hydroxy-3,6,7,3',4',5'-hexamethoxyflavone (5) and luteolin (6) and a known dolabellane diterpene (1R,2R,3E,7E,11R,12S)-2-O-acetyl-16-O-(3-hydroxy-3-methylglutaryl)-dolabella-3,7-dien-2,16,18-triol (7). The active parts of the MeOH fraction afforded the previously unreported 3'-methoxy-3,5,4'-trihydroxy flavone-7-neohesperidoside (8) and a known megastigmane norterpene; (6S,9R)-roseoside (9).
Abdel-Kader
Maged Saad
MS
Department of Pharmacognosy, College of Pharmacy, King Saud University, P.O. Box 2457, Riyadh 11451, Kingdom of Saudi Arabia. mpharm101@hotmail.com
Alqasoumi
Saleh Ibrahim
SI
Al-Taweel
Areej Mohammad
AM
D000410:Alanine Transaminase / Q000097:blood; D000469:Alkaline Phosphatase / Q000097:blood; D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood; D001663:Bilirubin / Q000378:metabolism; D002240:Carbohydrate Sequence; D056486:Chemical and Drug Induced Liver Injury / Q000473:pathology / Q000517:prevention & control*; D002725:Chloroform / Q000737:chemistry; D031485:Cleome / Q000737:chemistry*; D000431:Ethanol; D008099:Liver / Q000473:pathology; D008111:Liver Function Tests; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D000432:Methanol / Q000737:chemistry; D008969:Molecular Sequence Data; D010936:Plant Extracts / Q000737:chemistry; D020011:Protective Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D012997:Solvents; D013056:Spectrophotometry, Ultraviolet
D010936:Plant Extracts; D020011:Protective Agents; D012997:Solvents; D000431:Ethanol; D002725:Chloroform; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D000469:Alkaline Phosphatase; D001663:Bilirubin; D000432:Methanol