[Genotype and haplotype analysis of killer cell immunoglobulin-like receptors in ankylosing spondylitis].
OBJECTIVE
To investigate the association of killer cell immunoglobulin-like receptors (KIR) genotype and haplotype with ankylosing spondylitis (AS).


METHODS
Peripheral blood samples were collected from 105 AS patients, 62 patients of osteoarthritis (OA), and 412 randomly selected healthy controls. Polymerase chain reaction with sequence-specific primers (PCR-SSP) was used to detect the KIR genotype and haplotype.


RESULTS
The genotype frequency of 3DL3-2DL3-2DL1-2DP1-2DL4-3DL1-2DS4-3DL2 (6.67%) was significantly lower in the AS patients than in the control subjects (20.15%) and OA patients (17.74%, P = 0.001, 0.037 respectively). The genotype frequency of 3DL3-2DL3-2DL2-2DL1-2DP1-2DLA-3DL1-2DL5-2DS1-2DS2-2DS3-2DS4-2DS5-3DS1-3DL2 and 3DL3-2DL3-2DL2- 2DL1-2DP1-2DL4-3DL1-2DL5-2DS1-2DS4-3DL2 of the AS patients (9.52%, 5.71%)was significantly higher than that of the controls(2.18%, 0.49%; P = 0.001, 0.001), and these two genotypes were not detected in the OA patients. There were not significant differences in the haplotypes A and B among the AS patients, OA patients, and healthy controls.


CONCLUSIONS
KIR genotypes may be associated with the susceptibility to AS.
Zhang
Bing-Chang
BC
Department of Clinical Laboratory, Shandong Provincial Hospital Affiliated to Shandong University, Jinan 250021, China.
Liu
Yun
Y
Jiao
Yu-Lian
YL
Zhao
Yue-Ran
YR
Li
Jian-Feng
JF
D000293:Adolescent; D000328:Adult; D016022:Case-Control Studies; D002648:Child; D005260:Female; D005787:Gene Frequency; D005838:Genotype; D006239:Haplotypes; D006801:Humans; D008297:Male; D008875:Middle Aged; D010003:Osteoarthritis / Q000235:genetics / Q000276:immunology; D054340:Receptors, KIR / Q000235:genetics*; D017422:Sequence Analysis, DNA; D013167:Spondylitis, Ankylosing / Q000235:genetics* / Q000276:immunology*; D055815:Young Adult
D054340:Receptors, KIR