title: Characterisation of eGFP-transgenic BALB/c mouse strain established by lentiviral transgenesis.
abstract: Lentiviral technology is a powerful tool for the creation of stable transgenic animals. However, uncertainties have remained whether constitutive promoters resist long-term silencing. We used concentrated HIV-1 based lentiviral vectors to create stable transgenic BALB/c mice by perivitelline injection. In our vectors eGFP expression was driven by the human EF1alpha promoter. The established transgenic animals were analyzed for eGFP expression by in vivo fluorescence imaging, PCR, histology and flow-cytometry. eGFP expression showed even distribution without mosaicism; however, tissue-dependent differences of eGFP expression were observed. Up to the sixth generation only one newborn showed eGFP inactivation. eGFP + transgenic bone marrow cells efficiently provided long-term haemopoietic repopulation in radiation chimeras, regenerating all bone marrow-derived lineages with eGFP + cells with distinct eGFP expression profiles. The established eGFP + BALB/c mouse strain is expected to be extremely useful in various immunological experiments.
authors.affiliation: Department of Immunology and Biotechnology, University of Pecs, Pecs, Hungary.
authors.forename: Krisztián
authors.initials: K
authors.lastname: Kvell
authors.forename: Tamás
authors.initials: T
authors.lastname: Czömpöly
authors.forename: László
authors.initials: L
authors.lastname: Hiripi
authors.forename: Péter
authors.initials: P
authors.lastname: Balogh
authors.forename: József
authors.initials: J
authors.lastname: Kóbor
authors.forename: Lilla
authors.initials: L
authors.lastname: Bodrogi
authors.forename: Judit E
authors.initials: JE
authors.lastname: Pongrácz
authors.forename: William A
authors.initials: WA
authors.lastname: Ritchie
authors.forename: Zsuzsanna
authors.initials: Z
authors.lastname: Bosze
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D004526:Efficiency; D005260:Female; D020868:Gene Silencing / Q000502:physiology; D018014:Gene Transfer Techniques; D049452:Green Fluorescent Proteins / Q000235:genetics*; D040582:Inheritance Patterns; D016086:Lentivirus / Q000235:genetics*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008822:Mice, Transgenic / Q000235:genetics*; D011993:Recombinant Fusion Proteins / Q000235:genetics
chemical_list: D011993:Recombinant Fusion Proteins; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins