Quantitative real-time polymerase chain reaction for the core facility using TaqMan and the Perkin-Elmer/Applied Biosystems Division 7700 Sequence Detector.
Quantitative real-time polymerase chain reaction (PCR) using the Perkin Elmer/Applied Biosystems Division 7700 Sequence Detector provides an accurate method for determination of levels of specific DNA and RNA sequences in tissue samples. It is based on detection of a fluorescent signal produced proportionally during amplification of a PCR product. Turn-around time for data acquisition and analysis by real-time PCR with the 7700 model is short, and results are more reliable than by traditional PCR methods. This technology can be successfully offered as a service in a core faculty setting.
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Nucleic Acid Facility, Life Science Consortium, The Pennsylvania State University, University Park, PA 16802, USA.