Relationship between electropherotypes and VP7/VP4 genotypes of group A rotaviruses detected between 2000 and 2007 in Tunisian children.
BACKGROUND
Rotaviruses are the most frequent agents associated with diarrhoea in children worldwide. Analysis of mobility of the 11 segments of genomic RNA by polyacrylamide gel electrophoresis (PAGE) yields a pattern which is characteristic for a particular rotavirus isolate. The group A rotaviruses can be further characterized by analysis of VP7 and VP4 genes specificities, responsible for rotavirus classification into G and P genotypes, respectively. The aim of the present study was to detect a relationship between electropherotype pattern and molecular characteristics of the rotavirus strains.


METHODS
Were analyzed 278 rotavirus-positive specimens by PAGE and G/P-genotyped by multiplex semi-nested RT-PCR. Pearson's correlation tests were used for statistical analysis.


RESULTS
Twelve different electropherotypes were visualized, eight with a long profile (186 cases) and four with a short one (87 cases). Concerning VP7 types, G2 viral strains were found to be predominant and were detected in 91 specimens (32.7%). Strains with G1, G3, G4, G8 and G9 specificities were detected in 62 (22.3%), 82 (29.5%), 13 (4.7%), two (0.7%) and seven cases (2.5%), respectively. The results of VP4 genotyping showed a predominance of P[8] genotype which comprised half of the strains identified (139 cases, 50%). VP4 P[4], P[6] and P[11] were found in 83 (29.9%), 31 (11.1%) and 11 (4.0%) specimens, respectively. A high rate of mixed strains was also found (1.8% mixed electropherotypes, 7.6% G-mixed and 5% P-mixed strains). Electropherotype pattern of rotavirus strains was significantly correlated with VP7 genotype (p=0.018) and with VP4 genotype specificities (p<0.001).
Chouikha
A
A
Laboratory of Transmitted Diseases (MDT-01), Faculty of Pharmacy, Monastir, Tunisia.
Fodha
I
I
Ben Hadj Fredj
M
M
Ardhaoui
M
M
Teleb
N
N
Brini
I
I
Messaadi
F
F
Mastouri
M
M
Sfar
T
T
Hachicha
M
M
Kammoun
T
T
Bouaaziz
A
A
Amri
F
F
Harbi
A
A
Zribi
M
M
Bousnina
S
S
Khemakhem
A
A
Boujaafar
N
N
Trabelsi
A
A
Steele
A D
AD
D000956:Antigens, Viral / Q000032:analysis* / Q000235:genetics; D036022:Capsid Proteins / Q000032:analysis* / Q000235:genetics; D002648:Child; D003967:Diarrhea / Q000453:epidemiology / Q000821:virology*; D004591:Electrophoresis, Polyacrylamide Gel; D005243:Feces / Q000821:virology; D005838:Genotype; D006801:Humans; D012367:RNA, Viral / Q000032:analysis* / Q000235:genetics; D012401:Rotavirus / Q000737:chemistry / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D012400:Rotavirus Infections / Q000453:epidemiology / Q000821:virology*; D016622:Silver Staining; D014416:Tunisia / Q000453:epidemiology
D000956:Antigens, Viral; D036022:Capsid Proteins; D012367:RNA, Viral; C060339:VP4 protein, Rotavirus; C050779:VP7 protein, Rotavirus