title: Separation of structural, geometrical and optical isomers of epoxycarotenoids using triacontyl-bonded stationary phases.
abstract: The efficiency of C30 stationary phases in the separation of carotenes and diverse hydroxycarotenoids has been the subject of several studies. However, little is known concerning their ability to resolve epoxycarotenoids isomers, whose study is of great importance due to the functions they serve and the information they can reveal concerning the processing of foods. We have concluded that C30 columns provide an excellent separation of structural, geometrical and optical isomers of epoxycarotenoids and that the presence of 5,8-epoxide groups leads to a better shape recognition, to the extent that over 10 geometrical-optical isomers of 5,8-epoxycarotenoids have been separated. Additionally, it was observed that these carotenoids elute later than their 5,6-epoxide counterparts, albeit the latter have a longer chromophore.
authors.affiliation: Laboratory of Food Color & Quality, Department of Nutrition and Food Science, Faculty of Pharmacy, University of Seville, Seville, Spain.
authors.forename: Antonio J
authors.initials: AJ
authors.lastname: Meléndez-Martínez
authors.forename: Maria L
authors.initials: ML
authors.lastname: Escudero-Gilete
authors.forename: Isabel M
authors.initials: IM
authors.lastname: Vicario
authors.forename: Francisco J
authors.initials: FJ
authors.lastname: Heredia
mesh_terms: D002338:Carotenoids / Q000737:chemistry* / Q000302:isolation & purification*; D002853:Chromatography, Liquid / Q000379:methods*; D004852:Epoxy Compounds / Q000737:chemistry* / Q000302:isolation & purification*; D015394:Molecular Structure; D013237:Stereoisomerism
chemical_list: D004852:Epoxy Compounds; D002338:Carotenoids