Genic expression of the uterine leiomyoma in reproductive-aged women after treatment with goserelin.
OBJECTIVE
To identify the genes presenting different expression in uterine leiomyomas after goserelin treatment.


METHODS
Retrospective analyses of tissue obtained in a prospective clinical study.


METHODS
School of Medicine of the University of São Paulo.


METHODS
30 nulliparous black women aged 20 to 45 years with symptoms of uterine leiomyoma, uterine volume over 300 mL, and surgical indications for myomectomy.


METHODS
Fifteen patients were given a monthly dose of 3.6 mg of goserelin over 3 months before surgery (group A), and 15 patients underwent surgery without any previous treatment (group B). Five random samples from each group were analyzed using the microarray technique with the Affymetrix platform (GeneChip Rat Genome 230 2.0 Array).


METHODS
Quantification of transcript expression levels of uterine fibroids in patients treated or not treated with goserelin.


RESULTS
Of the total of 47,000 sequences that were analyzed, representing approximately 38,500 human genes already characterized, we found a differential expression of 174 genes. Of these, 70 were up-regulated (33 genes with known function) and 104 were down-regulated (65 genes with known function) in samples from group A (treated) when compared with group B (nontreated).


CONCLUSIONS
The genic expression of uterine leiomyomas changes in women who have had goserelin treatment when compared with nontreated patients.
Borsari
Rodrigo
R
LIM-58 of the Division of Gynecology, Department of Obstetrics and Gynecology, School of Medicine, Faculdade de Medicina da Universidade de São Paulo (FMUSP), São Paulo, Brazil. roborsari@hotmail.com
Bozzini
Nilo
N
Junqueira
Consuelo Rodrigues
CR
Soares
José Maria
JM
Hilário
Sandro Garcia
SG
Baracat
Edmund C
EC
D000328:Adult; D000367:Age Factors; D018931:Antineoplastic Agents, Hormonal / Q000494:pharmacology / Q000627:therapeutic use; D005260:Female; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects*; D017273:Goserelin / Q000494:pharmacology* / Q000627:therapeutic use; D006801:Humans; D007889:Leiomyoma / Q000188:drug therapy / Q000235:genetics* / Q000473:pathology; D008875:Middle Aged; D012098:Reproduction / Q000502:physiology; D012189:Retrospective Studies; D046888:Tissue Array Analysis; D014594:Uterine Neoplasms / Q000188:drug therapy / Q000235:genetics* / Q000473:pathology; D055815:Young Adult
D018931:Antineoplastic Agents, Hormonal; D017273:Goserelin