title: [The inhibitive effects of 5-fluorouracil-loaded polylactic acid nanoparticles on human Tenon's capsule fibroblast in vitro].
abstract: OBJECTIVE
  To investigate the inhibitive effects of 5-fluorouracil-loaded polylactic acid nanoparticles (5-FU-PLA-NPs) on human Tenon's capsule fibroblasts in vitro.
  
  
  METHODS
  This paper was experimental study. MTT assay was performed to estimate the effects of 0.1 mg/L, 1.0 mg/L, 10.0 mg/L, 100.0 mg/L, 1000.0 mg/L unloaded polylactic acid nanoparticles (PLA-NPs) on fibroblasts proliferation on 48 h, 72 h. MTT assay was performed to estimate the effects of 0.1 mg/L, 1.0 mg/L, 10.0 mg/L, 100.0 mg/L, 1000.0 mg/L original 5-FU and 5-FU-PLA-NPs on fibroblasts' proliferation on 7 consecutive days respectively. The inhibitory rate of the two drugs against the fibroblasts was calculated. Cells were exposed to 100 mg/L original 5-FU and 5-FU-PLA-NPs respectively for 7 days. Semi-quantitative RT-PCR was used to observe the mRNA expression of type III procollagen at the 1st day, 5th day and 7th day.
  
  
  RESULTS
  PLA-NPs had no cytotoxicity on the fibroblasts. Both 5-FU-PLA-NPs and original 5-FU could inhibit the proliferation of the fibroblasts and make the expression of type III procollagen mRNA lower in dose-dependent and time-dependent manner. The inhibitive effect of original 5-FU was more effective than 5-FU-PLA-NPs in the initial period, but the inhibitive effect of 5-FU-PLA-NPs was more effective than original 5-FU in long time.
  
  
  CONCLUSIONS
  PLA has good biocompatibility and safety, and can be used as a carrier of ophthalmic drugs. 5-FU-PLA-NPs shows slow-releasing function. 5-FU-PLA-NPs can be proposed as a potential controlled and targeted ophthalmic delivery system for the treatment of antifibrosis after glaucoma filtering surgery.
authors.affiliation: Department of Ophthalmology, Second Hospital Affiliated to Sun Yatsun University, Guangzhou 510020, China. lyqglp@163.com
authors.forename: Yu-Qing
authors.initials: YQ
authors.lastname: Lan
authors.forename: Xin
authors.initials: X
authors.lastname: Song
authors.forename: Jian-Hui
authors.initials: JH
authors.lastname: Xiao
authors.forename: Ye-Hong
authors.initials: YH
authors.lastname: Zhuo
authors.forename: Hui
authors.initials: H
authors.lastname: Guo
authors.forename: Chi
authors.initials: C
authors.lastname: Zhang
authors.forename: Kai-Hong
authors.initials: KH
authors.lastname: Huang
mesh_terms: D049109:Cell Proliferation; D002478:Cells, Cultured; D005123:Eye / Q000166:cytology; D005205:Fascia / Q000166:cytology / Q000187:drug effects*; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D005472:Fluorouracil / Q000008:administration & dosage / Q000494:pharmacology*; D006801:Humans; D019344:Lactic Acid; D053758:Nanoparticles; D011091:Polyesters; D011108:Polymers
chemical_list: D011091:Polyesters; D011108:Polymers; D019344:Lactic Acid; C033616:poly(lactide); D005472:Fluorouracil