title: Whole-blood polymerase chain reaction and restriction fragment length polymorphism: a simplified method by microwave irradiation.
abstract: OBJECTIVE
  The aim of the present study was to develop a simple, quick and cheap method to process whole-blood samples for the molecular techniques polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) without the use of expensive reagents or sophisticated machines.
  
  
  METHODS
  Venous whole-blood samples were collected from 40 individuals. The samples were frozen at -80 degrees C, and then rapidly thawed at 37 degrees C. Each sample was incubated with distilled water, then boiled in a microwave and centrifuged. The supernatant was taken directly for PCR and RFLP. For comparison, PCR and RFLP were performed on DNA purified from the same samples using the phenol-chloroform method and two commercial DNA extraction kits.
  
  
  RESULTS
  PCR/RFLP results using the presented method were qualitatively similar to those obtained by DNA extracted using the other three methods.
  
  
  CONCLUSIONS
  The presented method proved to be a simpler and cheaper way of processing whole-blood samples for PCR and RFLP analyses.
authors.affiliation: Department of Medical Laboratory Sciences, Faculty of Allied Health Sciences, Kuwait University, Sulaibekhat 90805, Kuwait. mehrez@hsc.edu.kw
authors.forename: Mehrez M
authors.initials: MM
authors.lastname: Jadaon
authors.forename: Ali A
authors.initials: AA
authors.lastname: Dashti
authors.forename: Hend L
authors.initials: HL
authors.lastname: Lewis
authors.forename: Fatema M
authors.initials: FM
authors.lastname: Habeeb
mesh_terms: D054458:Amplified Fragment Length Polymorphism Analysis; D004247:DNA / Q000097:blood / Q000302:isolation & purification; D004587:Electrophoresis, Agar Gel; D006801:Humans; D007962:Leukocytes / Q000737:chemistry; D008872:Microwaves; D016133:Polymerase Chain Reaction / Q000379:methods*; D012150:Polymorphism, Restriction Fragment Length
chemical_list: D004247:DNA