Effect of polypeptide from Chlamys farreri on UVB-induced ROS/NF-kappaB/COX-2 activation and apoptosis in HaCaT cells.
Polypeptide from Chlamys farreri (PCF) is a novel marine polypeptide compound isolated from gonochoric Chinese scallop Chlamys farreri, this study we further investigate the mechanisms of PCF exerting its anti-apoptotic effect. The results indicated that PCF, ROS scavenger NAC and NF-kappaB inhibitor MG132 effectively inhibited UVB-induced HaCaT cells apoptosis. PCF (2.84mM) showed potential ROS scavenging activities in a kinetic process. PCF (1.42-5.69mM) dose-dependently increased the expressions of Cu, Zn-SOD, CAT and GPx meanwhile decreased the expressions of p-NF-kappaB/p65 and COX-2 in UVB-induced HaCaT cells. Additionally, pretreatment with NAC significantly declined the generation of ROS and the expression of p-NF-kappaB/p65. We concluded that ROS, NF-kappaB and COX-2 are involved in UVB-induced HaCaT cells apoptosis, PCF exerts its protective effects via scavenging ROS, increasing the expression of antioxidative enzymes and inhibition the activation of NF-kappaB and COX-2.
Liu
XiaoJin
X
Medical College, Qingdao University, Boya Building, Qingdao, China.
Shi
ShaoTing
S
Ye
JunLi
J
Liu
LeQian
L
Sun
Mi
M
Wang
ChunBo
C
D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D015153:Blotting, Western; D002460:Cell Line; D051546:Cyclooxygenase 2 / Q000187:drug effects* / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D015536:Down-Regulation; D004789:Enzyme Activation / Q000187:drug effects*; D006801:Humans; D015603:Keratinocytes / Q000737:chemistry; D016328:NF-kappa B / Q000378:metabolism*; D049897:Pectinidae / Q000737:chemistry*; D010455:Peptides / Q000737:chemistry / Q000494:pharmacology*; D017382:Reactive Oxygen Species; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014466:Ultraviolet Rays
D016328:NF-kappa B; D010455:Peptides; D017382:Reactive Oxygen Species; D051546:Cyclooxygenase 2; C496540:PTGS2 protein, human