The genetics of green thorax, a new larval colour mutant, non-linked with ruby - eye locus in the malaria mosquito, Anopheles stephensi.
OBJECTIVE
Anopheles stephensi, an important vector of malaria continues to be distributed widely in the Indian subcontinent. The natural vigour of the species combined with its new tolerance, indeed resistance to insecticides has made it obligatory that we look for control methods involving genetic manipulation. Hence, there is an immediate need for greater understanding of the genetics of this vector species. One of the requirements for such genetic studies is the establishment of naturally occurring mutants, establishment of the genetic basis for the same and use of such mutants in the genetic transformation studies and other genetic control programme(s). This paper describes the isolation and genetic studies of a larval colour mutant, green thorax (gt), and linkage studies involving another autosomal recessive mutant ruby- eye (ru) in An. stephensi.


METHODS
After the initial discovery, the mutant green thorax was crossed inter se and pure homozygous stock of the mutant was established. The stock of the mutant ruby- eye, which has been maintained as a pure stock in the laboratory. Crosses were made between the wild type and mutant, green thorax to determine the mode of inheritance of green thorax. For linkage studies, crosses were made between the mutant green thorax and another autosomal recessive mutant ruby-eye. The percentage cross-over was calculated for the genes linkage relationship for gt and gt ru.


RESULTS
Results of crosses between mutant and wild type showed that the inheritance of green thorax (gt) in An. stephensi is monofactorial in nature. The gt allele is recessive to wild type and is autosomal. The linkage studies showed no linkage between ru and gt.


CONCLUSIONS
The mutant gt represents an excellent marker for An. stephensi as it is expressed in late III instar stage of larvae and is prominent in IV instar and pupal stages with complete penetrance and high viability. The said mutant could be easily identified without the aid of a microscope. This mutant can be used extensively to conduct basic and applied research. The mutant has been maintained in two large cages in our laboratory.
Sanil
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Centre for Applied Genetics, Bangalore University, J.B. Campus, Bengaluru & Janardhana Foundation, Nagadevanahalli,Jnanabharathi Post, Bengaluru, India.
Shetty
N J
NJ
D000818:Animals; D000852:Anopheles / Q000235:genetics* / Q000254:growth & development; D054910:Compound Eye, Arthropod; D003433:Crosses, Genetic; D005127:Eye Color / Q000235:genetics; D005260:Female; D017344:Genes, Insect; D005808:Genes, Recessive; D008040:Genetic Linkage; D007303:Insect Vectors / Q000235:genetics; D007814:Larva / Q000033:anatomy & histology / Q000235:genetics / Q000502:physiology; D008288:Malaria / Q000635:transmission; D008297:Male; D009154:Mutation; D017956:Photoreceptor Cells, Invertebrate; D010858:Pigmentation / Q000235:genetics*