cDNA cloning and mRNA expression of bovine GPR39.
GPR39 is an orphan G protein-coupled receptor that is thought to be involved in gastrointestinal and metabolic function. In this study, we cloned bovine GPR39 cDNA that encoded 462 amino acids showing high sequence homology to other mammalian GPR39 proteins. Real-time PCR showed expression of GPR39 mRNA in the liver, kidney, abomasums, small intestine, colon, rectum and uterus, with the highest level in the abomasums. Significant promoter activity was observed within the -2.3 kb 5'-upstream region of bovine GPR39 gene with human colon carcinoma-derived CACO-2 cells. These findings suggest that GPR39 may have important roles in gastrointestinal and metabolic functions in bovines as in other mammals.
Yamamoto
Ichiro
I
School of Veterinary Medicine, Nippon Veterinary and Life Science University, Japan.
Kimura
Nobuhiro
N
Arai
Toshiro
T
Tanaka
Minoru
M
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D018938:Caco-2 Cells; D002417:Cattle / Q000235:genetics*; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D005260:Female; D006801:Humans; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D043562:Receptors, G-Protein-Coupled / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D016415:Sequence Alignment
D018076:DNA, Complementary; D012333:RNA, Messenger; D043562:Receptors, G-Protein-Coupled