title: Infrared radiation influence on molt and regeneration of Neohelice granulata Dana, 1851 (Grapsidae, Sesarminae).
abstract: This paper analyzes the influence of infrared radiation (IR) on regeneration, after autotomy of limb buds of Neohelice granulata and consequently the time molt. Eyestalks were ablated to synchronize the start of molt. Afterward, animals were autotomized of five pereopods and divided into control and irradiated groups. The irradiated group was treated for 30 min daily until molt. Limb buds from five animals of days 4, 16 and 20 were collected and histological sections were made from them. These sections were photographed and chitin and epithelium content measured. Another group was made, and after 15 days limb buds were extracted to analyze mitochondrial enzymatic activity from complex I and II. The irradiated group showed a significant reduction in molt time (19.38+/-1.22 days) compared with the control group (32.69+/-1.57 days) and also a significant increase in mitochondrial complex I (388.9+/-27.94%) and II (175.63+/-7.66%) in the irradiated group when compared with the control group (100+/-17.90; 100+/-7.82, respectively). However, these effects were not accompanied by histological alterations in relation to chitin and epithelium. This way, it was possible to demonstrate that IR increases complex I and II activity, reduces the time molt and consequently increases the appendage regeneration rate.
authors.affiliation: Instituto de Ciências Biológicas, Fundação Universidade Federal do Rio Grande (FURG), Rio Grande, Brazil.
authors.forename: Vinícius Cunha
authors.initials: VC
authors.lastname: Gonzalez
authors.forename: Alexandre Baldez
authors.initials: AB
authors.lastname: Lena
authors.forename: Wendel Aculha
authors.initials: WA
authors.lastname: Espindola
authors.forename: Ana Paula
authors.initials: AP
authors.lastname: de Souza Votto
authors.forename: Marianna Basso
authors.initials: MB
authors.lastname: Jorge
authors.forename: Daza
authors.initials: D
authors.lastname: de Moraes Vaz Batista Filgueira
authors.forename: Adalto
authors.initials: A
authors.lastname: Bianchini
authors.forename: Antônio
authors.initials: A
authors.lastname: Sérgio Varela Júnior
authors.forename: Luiz Eduardo Maia
authors.initials: LE
authors.lastname: Nery
authors.forename: Gilma Santos
authors.initials: GS
authors.lastname: Trindade
mesh_terms: D000818:Animals; D003445:Crustacea / Q000502:physiology / Q000528:radiation effects*; D007259:Infrared Rays; D018878:Limb Buds / Q000254:growth & development / Q000502:physiology / Q000528:radiation effects; D018978:Molting; D012038:Regeneration