In silico analysis for identification of tick phagotopes selected by phage-displayed libraries.
Phage display techniques have been widely employed to map epitope structures which have served as the basis for developing molecular vaccines. We have applied this technique to map specific epitopes of Rhipicephalus (Boophilus) microplus. In the present study, we have identified the potential immunogens using a process in which the selected phage clones were analyzed through bioinformatics, prior to final field tests. The present study demonstrates the feasibility of identifying important R. (B.) microplus phagotopes for vaccine development through screening of phage-displayed random peptide libraries and bioinformatics tools.
Prudencio
Carlos Roberto
CR
Laboratório de Bionanotecnologia, Instituto de Genética e Bioquímica, Universidade Federal de Uberlândia--UFU, Brazil. crprudencio@gmail.com
Nascimento
Rafael
R
Filho
Moacir Marchiori
MM
Marra
Andrea de Oliveira Marques
Ade O
de Souza
Guilherme Rocha Lino
GR
Almeida
Juliana Franco
JF
Cardoso
Rone
R
Szabó
Matias Pablo Juan
MP
Goulart
Luiz Ricardo
LR
D000818:Animals; D019151:Peptide Library; D013987:Ticks / Q000145:classification*
D019151:Peptide Library