Effects of theaflavins on melanin biosynthesis in mouse b16 melanoma cells.
In this study, we examined the effects of theaflavins, polyphenols in black tea, on alpha melanocyte-stimulating hormone (alphaMSH)-induced melanogenesis in mouse B16 melanoma cells. Among theaflavins, theaflavin-3,3'-digallate (TF-3) exhibited a potent inhibitory effect on melanogenesis. Our results indicate that the inhibitory effect of TF-3 on melanogenesis is due to suppression of tyrosinase protein and mRNA levels.
Yamaoka
Yuriko
Y
Aichikonan Junior College, Konan, Aichi, Japan.
Ohguchi
Kenji
K
Itoh
Tomohiro
T
Nozawa
Yoshinori
Y
Akao
Yukihiro
Y
D000818:Animals; D001483:Base Sequence; D044946:Biflavonoids / Q000494:pharmacology*; D002392:Catechin / Q000494:pharmacology*; D045744:Cell Line, Tumor; D017931:DNA Primers; D004591:Electrophoresis, Polyacrylamide Gel; D008543:Melanins / Q000096:biosynthesis*; D008546:Melanoma, Experimental / Q000201:enzymology / Q000378:metabolism* / Q000473:pathology; D051379:Mice; D014442:Monophenol Monooxygenase / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction
D044946:Biflavonoids; D017931:DNA Primers; D008543:Melanins; D012333:RNA, Messenger; C056068:theaflavin; D002392:Catechin; D014442:Monophenol Monooxygenase