title: In Vivo Measurements of Tumor Metabolism and Growth after Administration of Enzastaurin Using Small Animal FDG Positron Emission Tomography.
abstract: Background. The use of 2-[(18)F]fluoro-2-deoxy-D-glucose ([(18)F]FDG) may help to establish the antitumor activity of enzastaurin, a novel protein kinase C-beta II (PKC-betaII) inhibitor, in mouse xenografts. Methods. The hematologic cell line RAJI and the solid tumor cell line U87MG were each implanted in NOD/SCID mice. Standard tumor growth measurements and [(18)F]FDG PET imaging were performed weekly for up to three weeks after tumor implantation and growth. Results. Concomitant with caliper measurements, [(18)F]FDG PET imaging was performed to monitor glucose metabolism. Heterogeneity of glucose uptake in various areas of the tumors was observed after vehicle or enzastaurin treatment. This heterogeneity may limit the use of [(18)F]FDG PET imaging to measure enzastaurin-associated changes in xenograft tumors. Conclusion. [(18)F]FDG PET imaging technique does not correlate with standard caliper assessments in xenografts to assess the antitumor activity of enzastaurin. Future studies are needed to determine the use of [(18)F]FDG PET imaging in preclinical models.
authors.affiliation: Section of Pediatric Hematology & Oncology, Herman B Wells Center for Pediatric Research, Indiana University Simon Cancer Center, 1044 West Walnut Street R4 321, Indianapolis, IN 46202, USA.
authors.forename: Karen E
authors.initials: KE
authors.lastname: Pollok
authors.forename: Michael
authors.initials: M
authors.lastname: Lahn
authors.forename: Nathan
authors.initials: N
authors.lastname: Enas
authors.forename: Ann
authors.initials: A
authors.lastname: McNulty
authors.forename: Jeremy
authors.initials: J
authors.lastname: Graff
authors.forename: Shanbao
authors.initials: S
authors.lastname: Cai
authors.forename: Jennifer R
authors.initials: JR
authors.lastname: Hartwell
authors.forename: Aaron
authors.initials: A
authors.lastname: Ernstberger
authors.forename: Donald
authors.initials: D
authors.lastname: Thornton
authors.forename: Les
authors.initials: L
authors.lastname: Brail
authors.forename: Gary
authors.initials: G
authors.lastname: Hutchins