Role of Na+/Ca2+ exchanger-mediated Ca2+ entry in pressure-induced myogenic constriction in rat posterior cerebral arteries.
The involvement of Ca2+ entry via the Na+/Ca2+ exchanger (NCX) in myogenic constriction of rat posterior cerebral arteries was investigated. RT-PCR identified mRNA for NCX1, 2, and 3 in the arteries. Na+ removal increased [Ca2+]i, which was reduced by the NCX inhibitor SEA0400. SEA0400 inhibited the development, but not the steady-state, of pressure-induced myogenic constriction, whereas it decreased both the initial and sustained phases of [Ca2+]i elevation. These results suggest that Ca2+ entry via NCX is involved in the development, but not the steady-state, of pressure-induced myogenic constriction.
Kashihara
Toshihide
T
Department of Pharmacology, Graduate School of Pharmaceutical Sciences, University of Shizuoka, Japan.
Nakayama
Koichi
K
Matsuda
Toshio
T
Baba
Akemichi
A
Ishikawa
Tomohisa
T
D000792:Angiography; D000814:Aniline Compounds / Q000494:pharmacology; D000818:Animals; D002118:Calcium / Q000378:metabolism*; D008297:Male; D010647:Phenyl Ethers / Q000494:pharmacology; D020769:Posterior Cerebral Artery / Q000187:drug effects / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D020133:Reverse Transcriptase Polymerase Chain Reaction; D019831:Sodium-Calcium Exchanger / Q000037:antagonists & inhibitors / Q000378:metabolism*; D014661:Vasoconstriction / Q000187:drug effects / Q000502:physiology
D000814:Aniline Compounds; D010647:Phenyl Ethers; D012333:RNA, Messenger; C430918:SEA 0400; C110927:Slc8a2 protein, rat; C491102:Slc8a3 protein, rat; D019831:Sodium-Calcium Exchanger; C110374:sodium-calcium exchanger 1; D002118:Calcium