title: Recruitment of a SAP18-HDAC1 complex into HIV-1 virions and its requirement for viral replication.
abstract: HIV-1 integrase (IN) is a virally encoded protein required for integration of viral cDNA into host chromosomes. INI1/hSNF5 is a component of the SWI/SNF complex that interacts with HIV-1 IN, is selectively incorporated into HIV-1 (but not other retroviral) virions, and modulates multiple steps, including particle production and infectivity. To gain further insight into the role of INI1 in HIV-1 replication, we screened for INI1-interacting proteins using the yeast two-hybrid system. We found that SAP18 (Sin3a associated protein 18 kD), a component of the Sin3a-HDAC1 complex, directly binds to INI1 in yeast, in vitro and in vivo. Interestingly, we found that IN also binds to SAP18 in vitro and in vivo. SAP18 and components of a Sin3A-HDAC1 complex were specifically incorporated into HIV-1 (but not SIV and HTLV-1) virions in an HIV-1 IN-dependent manner. Using a fluorescence-based assay, we found that HIV-1 (but not SIV) virion preparations harbour significant deacetylase activity, indicating the specific recruitment of catalytically active HDAC into the virions. To determine the requirement of virion-associated HDAC1 to HIV-1 replication, an inactive, transdominant negative mutant of HDAC1 (HDAC1(H141A)) was utilized. Incorporation of HDAC1(H141A) decreased the virion-associated histone deacetylase activity. Furthermore, incorporation of HDAC1(H141A) decreased the infectivity of HIV-1 (but not SIV) virions. The block in infectivity due to virion-associated HDAC1(H141A) occurred specifically at the early reverse transcription stage, while entry of the virions was unaffected. RNA-interference mediated knock-down of HDAC1 in producer cells resulted in decreased virion-associated HDAC1 activity and a reduction in infectivity of these virions. These studies indicate that HIV-1 IN and INI1/hSNF5 bind SAP18 and selectively recruit components of Sin3a-HDAC1 complex into HIV-1 virions. Furthermore, HIV-1 virion-associated HDAC1 is required for efficient early post-entry events, indicating a novel role for HDAC1 during HIV-1 replication.
authors.affiliation: Department of Genetics, Albert Einstein College of Medicine, Bronx, New York, United States of America.
authors.forename: Masha
authors.initials: M
authors.lastname: Sorin
authors.forename: Jennifer
authors.initials: J
authors.lastname: Cano
authors.forename: Supratik
authors.initials: S
authors.lastname: Das
authors.forename: Sheeba
authors.initials: S
authors.lastname: Mathew
authors.forename: Xuhong
authors.initials: X
authors.lastname: Wu
authors.forename: Kelvin P
authors.initials: KP
authors.lastname: Davies
authors.forename: Xuanling
authors.initials: X
authors.lastname: Shi
authors.forename: S-W Grace
authors.initials: SW
authors.lastname: Cheng
authors.forename: David
authors.initials: D
authors.lastname: Ott
authors.forename: Ganjam V
authors.initials: GV
authors.lastname: Kalpana
mesh_terms: D002352:Carrier Proteins / Q000378:metabolism*; D002460:Cell Line; D002868:Chromosomal Proteins, Non-Histone / Q000378:metabolism; D056970:Co-Repressor Proteins; D004268:DNA-Binding Proteins / Q000378:metabolism; D003627:Data Interpretation, Statistical; D019427:HIV Integrase / Q000378:metabolism*; D015497:HIV-1 / Q000378:metabolism / Q000502:physiology*; D056284:Histone Deacetylase 1; D006655:Histone Deacetylases / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D047468:Immunoprecipitation; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics / Q000378:metabolism; D016601:RNA-Binding Proteins; D012097:Repressor Proteins / Q000378:metabolism; D000071796:SMARCB1 Protein; D015302:Simian Immunodeficiency Virus / Q000378:metabolism; D056264:Sin3 Histone Deacetylase and Corepressor Complex; D014157:Transcription Factors / Q000378:metabolism; D014771:Virion / Q000378:metabolism; D014779:Virus Replication
chemical_list: D002352:Carrier Proteins; D002868:Chromosomal Proteins, Non-Histone; D056970:Co-Repressor Proteins; D004268:DNA-Binding Proteins; D034741:RNA, Small Interfering; D016601:RNA-Binding Proteins; D012097:Repressor Proteins; C498650:SAP18 protein, human; C099683:SIN3A transcription factor; D000071796:SMARCB1 Protein; C513266:SMARCB1 protein, human; D014157:Transcription Factors; D019427:HIV Integrase; C110550:HDAC1 protein, human; D056284:Histone Deacetylase 1; D006655:Histone Deacetylases; D056264:Sin3 Histone Deacetylase and Corepressor Complex