Detection of drug transporter expression using a 25-multiplex RT-PCR assay.
Membrane transporters play important roles in mediating chemoresistance and chemosensitivity of tumor cells. Five sets of 5 genes were designed to simultaneously detect drug transporter expression in a single reaction tube using multiplex RT-PCR for 25 genes, including 17 ABC transporters, one non-ABC transporter, 4 SLC transporters, 2 copper transporters and one housekeeping gene. We optimized the multiplex RT-PCR conditions using chemosensitive cancer cells and then validated the system using chemoresistant cancer cells. This reliable multiplex RT-PCR assay can be utilized not only to investigate anticancer drug-resistance mechanisms but also to estimate the efficacy of anticancer chemotherapy in the clinic.
Lee
Tae-Bum
TB
Research Center for Resistant Cells, Chosun University Medical School, Gwangju, 501-759, Korea.
Choi
Cheol-Hee
CH
D000970:Antineoplastic Agents / Q000378:metabolism; D045744:Cell Line, Tumor; D019008:Drug Resistance, Neoplasm; D020869:Gene Expression Profiling; D006801:Humans; D026901:Membrane Transport Proteins / Q000096:biosynthesis* / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*
D000970:Antineoplastic Agents; D026901:Membrane Transport Proteins