Use of coherent control for selective two-photon fluorescence microscopy in live organisms.
We demonstrate selective fluorescence We demonstrate selective fluorescence excitation of specific molecular species in live organisms by using coherent control of two-photon excitation. We have acquired quasi-simultaneous images in live fluorescently-labeled Drosophila embryos by rapid switching between appropriate pulse shapes. Linear combinations of these images demonstrate that a high degree of fluorophore selectivity is attainable through phase-shaping. Broadband phase-shaped excitation opens up new possibilities for single-laser, multiplex, in-vivo fluorescence microscopy.
Ogilvie
Jennifer P
JP
Débarre
Delphine
D
Solinas
Xavier
X
Martin
Jean-Louis
JL
Beaurepaire
Emmanuel
E
Joffre
Manuel
M