Expression profile and localization of mouse calcitonin (CT) sense/antisense transcripts in pre- and postnatal tissue development.
Calcitonin (CT) has been shown to have various functions including osteoclast activity and calcium and phosphorus metabolism in mammals. In the present study, we measured the amounts of CT mRNA in the mouse brain, liver, kidney, heart and testis at various development stages, 14 days post-coitum (dpc), 17-dpc, newborn, 1 week and 8 weeks (adult), using real-time PCR. In the brain and kidney, the amount of CT mRNA decreased with development. In the testis, elevated amounts were observed at 17-dpc and 8 weeks. In the liver, the amount increased from the 14 dpc embryo to newborn stage and then decreased. In the heart, elevated amounts were observed at 17-dpc. Additionally, the CT antisense transcript was determined using a modified RT-PCR and nucleotide sequencing in the present study. Organs with high mRNA expressions were examined for localization of transcripts using in situ hybridization. The CT sense and antisense transcripts in the 14 dpc brain were mainly localized in the mesencephalon. In the pre- and postnatal stages, sense and antisense transcripts were shown to exist rather uniformly in the kidney, heart, liver and testis. In the 17-dpc rib and thyroid lobe and the adult ovary, the sense and antisense transcripts were found to be densely localized.
Rezaeian
Abdol Hossein
AH
National Institute of Agrobiological Science, Ibaraki, Japan.
Nishibori
Masahide
M
Hiraiwa
Noriko
N
Yoshizawa
Midori
M
Yasue
Hiroshi
H
D000818:Animals; D000831:Animals, Newborn; D001921:Brain / Q000502:physiology; D002116:Calcitonin / Q000096:biosynthesis* / Q000235:genetics; D047108:Embryonic Development / Q000235:genetics / Q000502:physiology; D020869:Gene Expression Profiling; D006321:Heart / Q000502:physiology; D017403:In Situ Hybridization; D007668:Kidney / Q000502:physiology; D008099:Liver / Q000502:physiology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013737:Testis / Q000502:physiology; D014158:Transcription, Genetic
D012333:RNA, Messenger; D002116:Calcitonin