title: Differential induction of apoptosis of leukemic cells by rhizochalin, two headed sphingolipids from sponge and its derivatives.
abstract: Two-headed sphingolipids bear a certain similarity with sphingolipids in the structure and but differing from classical sphingolipids in alpha,omega-position of the basic groups. We analyzed the apoptotic effects of some two-headed sphingolipids including rhizochalin (Rhz) and its derivatives isolated from sponge Rhizochalina incrustata on human leukemia HL-60 cells. Direct addition of Rhz induced apoptosis of HL-60 cells. The aglycon of Rhz, which has no galactosyl residue, showed a stronger ability to induce apoptotic activity than Rhz. Rhz congeners with acetate derivatives only weakly induced apoptosis. The usual mitochondrial membrane permeability changes and the decrease of protein levels of procaspases-8, -9, and -3 correlated with the apoptotic activity of Rhzs. These results suggest that derivatives of two-headed sphingolipids potently induce apoptosis in mammalian cells when administered exogenously and this cell death was dependent on caspase activation pathways.
authors.affiliation: Department of Biochemistry, School of Medicine, Dong-A University, Busan, Korea.
authors.forename: Jun-O
authors.initials: JO
authors.lastname: Jin
authors.forename: Valeria
authors.initials: V
authors.lastname: Shastina
authors.forename: Joo-In
authors.initials: JI
authors.lastname: Park
authors.forename: Jin-Yeong
authors.initials: JY
authors.lastname: Han
authors.forename: Tatyana
authors.initials: T
authors.lastname: Makarieva
authors.forename: Sergey
authors.initials: S
authors.lastname: Fedorov
authors.forename: Valery
authors.initials: V
authors.lastname: Rasskazov
authors.forename: Valentin
authors.initials: V
authors.lastname: Stonik
authors.forename: Jong-Young
authors.initials: JY
authors.lastname: Kwak
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D000975:Antioxidants / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects*; D061945:Caspase Inhibitors; D020169:Caspases / Q000378:metabolism; D002453:Cell Cycle / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005233:Fatty Alcohols / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D006027:Glycosides / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D018922:HL-60 Cells; D006801:Humans; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D015394:Molecular Structure; D011161:Porifera / Q000737:chemistry*; D013107:Sphingolipids / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D013329:Structure-Activity Relationship
chemical_list: D000970:Antineoplastic Agents; D000975:Antioxidants; D061945:Caspase Inhibitors; D004791:Enzyme Inhibitors; D005233:Fatty Alcohols; D006027:Glycosides; D013107:Sphingolipids; C068290:rhizochaline; D020169:Caspases