title: UV Melting of G-Quadruplexes.
abstract: Absorbance versus temperature curves can provide information on the thermal stability of DNA or RNA quadruplexes. Quadruplex denaturation or renaturation can be followed by recording absorbance at 295 nm rather than 260 nm, the wavelength used to monitor duplex denaturation. This unit describes the use of absorbance versus temperature curves to determine melting temperatures (T(m) values) and model-dependent thermodynamic parameters.
authors.affiliation: Laboratoire de Biophysique, Inserm U565, Muséum National d'Histoire Naturelle, Paris, France.
authors.forename: Jean-Louis
authors.initials: JL
authors.lastname: Mergny
authors.affiliation: Laboratoire de Biophysique, Inserm U565, Muséum National d'Histoire Naturelle, Paris, France.
authors.forename: Laurent
authors.initials: L
authors.lastname: Lacroix
mesh_terms: D054856:G-Quadruplexes / Q000528:radiation effects*; D009691:Nucleic Acid Denaturation / Q000528:radiation effects*; D013056:Spectrophotometry, Ultraviolet / Q000379:methods; D013696:Temperature; D014466:Ultraviolet Rays