title: Proteomic analysis of Legionella-containing phagosomes isolated from Dictyostelium.
abstract: Legionella pneumophila, the agent of Legionnaires' disease, replicates intracellularly within specialized phagosomes of human macrophages and amoebae. In this study, we have developed a protocol for the isolation of Legionella-containing phagosomes from Dictyostelium discoideum. Cell fractionation, two-dimensional gel electrophoresis and MALDI-TOF MS combined with genomic data identified 157 phagosome host proteins. In addition to proteins with an evident role in phagosome maturation, we identified proteins for which a function remains to be elucidated. Possible interactions of coronin with cytosolic NADPH oxidase components and protein kinase C inhibitors which together may lead to an inhibition of phagosomal superoxide generation are discussed. Comparative proteomics of phagosomes containing highly virulent L. pneumophila Corby versus less virulent L. hackeliae revealed distinctive protein expression patterns, e.g., an abundance of RhoGDI in L. hackeliae degrading phagosomes versus little RhoGDI in L. pneumophila Corby replicative phagosomes. We present a kinetic dissection of phagosome maturation including the complex alterations of the phagosome protein composition. A reference flow chart suggests so far unrecognized consequences of infection for host cell physiology, actin degradation on phagosomes, and a putative cysteine proteinase inhibitor interference with lysosomal enzyme sorting and activation processes.
authors.affiliation: Institut für Mikrobiologie, Technische Universität Braunschweig, Braunschweig, Germany.
authors.forename: Olga
authors.initials: O
authors.lastname: Shevchuk
authors.forename: Christoph
authors.initials: C
authors.lastname: Batzilla
authors.forename: Sonja
authors.initials: S
authors.lastname: Hägele
authors.forename: Harald
authors.initials: H
authors.lastname: Kusch
authors.forename: Susanne
authors.initials: S
authors.lastname: Engelmann
authors.forename: Michael
authors.initials: M
authors.lastname: Hecker
authors.forename: Albert
authors.initials: A
authors.lastname: Haas
authors.forename: Klaus
authors.initials: K
authors.lastname: Heuner
authors.forename: Gernot
authors.initials: G
authors.lastname: Glöckner
authors.forename: Michael
authors.initials: M
authors.lastname: Steinert
mesh_terms: D000199:Actins / Q000378:metabolism; D000818:Animals; D002458:Cell Fractionation; D015853:Cysteine Proteinase Inhibitors / Q000378:metabolism; D004023:Dictyostelium / Q000737:chemistry* / Q000382:microbiology* / Q000502:physiology / Q000648:ultrastructure; D015180:Electrophoresis, Gel, Two-Dimensional; D023281:Genomics / Q000379:methods; D007875:Legionella / Q000254:growth & development*; D008247:Lysosomes / Q000201:enzymology / Q000378:metabolism; D008840:Microfilament Proteins / Q000378:metabolism; D046529:Microscopy, Electron, Transmission; D008954:Models, Biological; D019255:NADPH Oxidases / Q000494:pharmacology; D010588:Phagosomes / Q000737:chemistry* / Q000000981:diagnostic imaging / Q000378:metabolism / Q000382:microbiology*; D047428:Protein Kinase Inhibitors / Q000378:metabolism; D020543:Proteome / Q000032:analysis* / Q000302:isolation & purification; D040901:Proteomics / Q000379:methods; D015800:Protozoan Proteins / Q000032:analysis* / Q000302:isolation & purification / Q000378:metabolism; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013481:Superoxides / Q000378:metabolism; D014463:Ultrasonography
chemical_list: D000199:Actins; D015853:Cysteine Proteinase Inhibitors; D008840:Microfilament Proteins; D047428:Protein Kinase Inhibitors; D020543:Proteome; D015800:Protozoan Proteins; D013481:Superoxides; C072061:coronin proteins; D019255:NADPH Oxidases