title: Mycobacterium kyorinense sp. nov., a novel, slow-growing species, related to Mycobacterium celatum, isolated from human clinical specimens.
abstract: A novel, non-pigmented, slow-growing mycobacterium was identified on the basis of biochemical and nucleic acid analyses, as well as growth characteristics. Three isolates were cultured from clinical samples (two from sputum and one from pus in lymph nodes) obtained from three immunocompetent patients with infections. Bacterial growth occurred at 28-42 degrees C on Middlebrook 7H11-OADC agar. The isolates showed negative results for Tween hydrolysis, nitrate reductase, semiquantitative catalase, urease activity, 3 day arylsulfatase activity, pyrazinamidase, tellurite reduction and niacin accumulation tests, but positive results for 14 day arylsulfatase activity and heat-stable catalase tests. The isolates contained alpha-, keto-, and dicarboxymycolates in their cell walls. Sequence analysis revealed that all isolates had identical, unique 16S rRNA sequences. Phylogenetic analysis of the 16S rRNA, rpoB, hsp65 and sodA gene sequences confirmed that these isolates are unique but closely related to Mycobacterium celatum. DNA-DNA hybridization of the isolates demonstrated less than 50 % reassociation with M. celatum and Mycobacterium branderi. On the basis of these findings, a novel species designated Mycobacterium kyorinense sp. nov. is proposed. The type strain is KUM 060204(T) (=JCM 15038(T)=DSM 45166(T)).
authors.affiliation: Department of Laboratory Medicine, Kyorin University School of Medicine, 6-20-2 Shinkawa, Mitaka-shi, Tokyo 181-8611, Japan.
authors.forename: Mitsuhiro
authors.initials: M
authors.lastname: Okazaki
authors.forename: Kiyofumi
authors.initials: K
authors.lastname: Ohkusu
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Hata
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Ohnishi
authors.forename: Keiko
authors.initials: K
authors.lastname: Sugahara
authors.forename: Chizuko
authors.initials: C
authors.lastname: Kawamura
authors.forename: Nagatoshi
authors.initials: N
authors.lastname: Fujiwara
authors.forename: Sohkichi
authors.initials: S
authors.lastname: Matsumoto
authors.forename: Yukiko
authors.initials: Y
authors.lastname: Nishiuchi
authors.forename: Kouichi
authors.initials: K
authors.lastname: Toyoda
authors.forename: Hajime
authors.initials: H
authors.lastname: Saito
authors.forename: Shota
authors.initials: S
authors.lastname: Yonetani
authors.forename: Yoko
authors.initials: Y
authors.lastname: Fukugawa
authors.forename: Masayuki
authors.initials: M
authors.lastname: Yamamoto
authors.forename: Hiroo
authors.initials: H
authors.lastname: Wada
authors.forename: Akiko
authors.initials: A
authors.lastname: Sejimo
authors.forename: Akio
authors.initials: A
authors.lastname: Ebina
authors.forename: Hajime
authors.initials: H
authors.lastname: Goto
authors.forename: Takayuki
authors.initials: T
authors.lastname: Ezaki
authors.forename: Takashi
authors.initials: T
authors.lastname: Watanabe
mesh_terms: D000368:Aged; D001426:Bacterial Proteins / Q000235:genetics; D015373:Bacterial Typing Techniques; D004269:DNA, Bacterial / Q000032:analysis / Q000302:isolation & purification; D005260:Female; D006801:Humans; D007564:Japan; D008297:Male; D008826:Microbial Sensitivity Tests; D008875:Middle Aged; D008969:Molecular Sequence Data; D009161:Mycobacterium / Q000145:classification* / Q000235:genetics / Q000254:growth & development* / Q000302:isolation & purification; D009164:Mycobacterium Infections / Q000382:microbiology*; D009171:Mycolic Acids / Q000032:analysis; D009693:Nucleic Acid Hybridization; D010641:Phenotype; D010802:Phylogeny; D018410:Pneumonia, Bacterial / Q000382:microbiology*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013045:Species Specificity; D013183:Sputum / Q000382:microbiology
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D009171:Mycolic Acids; D012336:RNA, Ribosomal, 16S