title: Adhesion of human leukocytes on mixtures of hydroxyl- and methyl-terminated self-assembled monolayers: effect of blood protein adsorption.
abstract: The adhesion of human leukocytes to nanostructured surfaces with different chemical properties and the effect of protein adsorption were investigated. Self-assembled monolayers (SAMs) prepared with mixtures of methyl- and hydroxyl-terminated alkanethiols in different percentages on gold were used. The surfaces were pre-immersed in distinct protein solutions (human serum albumin, human fibrinogen, and autologous plasma). Adherent leukocytes were analyzed both by light and SEM. SAMs submitted to pre-immersion in plasma presented higher numbers of adherent leukocytes in the pure OH-terminated SAM, whereas methyl-terminated surfaces accounted for the lowest number of adherent cells. We observed a general increase in the number of adherent human leukocytes as the percentage of OH groups on the surface of the SAMs increased for all the pre-immersion conditions investigated. The number of adherent human leukocytes is highly influenced by the pre-immersion conditions used, and this observation is particularly relevant in the case of the methyl-terminated SAMs. The results obtained demonstrate that surface chemistry has a major influence in leukocyte adhesion to biomaterials, and that pre-immersion in protein solutions has a determinant effect in leukocyte adhesion.
authors.affiliation: Divisão de Biomateriais, INEB-Instituto de Engenharia Biomédica, Universidade do Porto, Porto 4150-180, Portugal. judite@ineb.up.pt
authors.forename: Judite N
authors.initials: JN
authors.lastname: Barbosa
authors.forename: M C L
authors.initials: MC
authors.lastname: Martins
authors.forename: Sidónio C
authors.initials: SC
authors.lastname: Freitas
authors.forename: Inês C
authors.initials: IC
authors.lastname: Gonçalves
authors.forename: Artur P
authors.initials: AP
authors.lastname: Aguas
authors.forename: Mário A
authors.initials: MA
authors.lastname: Barbosa
mesh_terms: D000327:Adsorption / Q000187:drug effects; D001798:Blood Proteins / Q000378:metabolism*; D002448:Cell Adhesion / Q000187:drug effects; D006046:Gold / Q000494:pharmacology; D006801:Humans; D017665:Hydroxyl Radical / Q000737:chemistry*; D007962:Leukocytes / Q000166:cytology* / Q000187:drug effects / Q000648:ultrastructure; D013499:Surface Properties / Q000187:drug effects
chemical_list: D001798:Blood Proteins; D017665:Hydroxyl Radical; D006046:Gold