title: Association of tenascin-W expression with mineralization in mouse calvarial development.
abstract: Tenascin-W is a tenascin family member that forms part of a complex extracellular matrix, and previous studies have suggested its association with osteogenesis. In the present study we investigated the roles of tenascin-W in osteogenesis. We found that tenascin-W is expressed in osteoblasts at the edge of the developing bone domain prior to mineralization in mouse fetuses. Expression of tenascin-W was induced during the course of mineralization of the Kusa-A1 osteoblast cell line. In the interfrontal suture of postnatal mice, the anterior portion remains patent and the posterior portion closes by 4 weeks of age. Tenascin-W expression was downregulated at 1 week of age in the posterior frontal suture, whereas in the anterior suture, expression was maintained until the mice reached 4 weeks of age. Fibroblast growth factor 2 (FGF2)-bead application to the mouse fetal skull by ex-utero surgery accelerated osteoblast differentiation, but inhibited mineralization with a downregulation of tenascin-W expression. These results suggest that tenascin-W is involved in osteoblast maturation (i.e. mineralization).
authors.affiliation: Section of Molecular Craniofacial Embryology, Tokyo Medical and Dental University Graduate School of Medical and Dental Sciences, Tokyo, Japan.
authors.forename: Ayako
authors.initials: A
authors.lastname: Mikura
authors.forename: Shigeru
authors.initials: S
authors.lastname: Okuhara
authors.forename: Masahiro
authors.initials: M
authors.lastname: Saito
authors.forename: Masato
authors.initials: M
authors.lastname: Ota
authors.forename: Koichi
authors.initials: K
authors.lastname: Ueda
authors.forename: Sachiko
authors.initials: S
authors.lastname: Iseki
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D001846:Bone Development; D002113:Calcification, Physiologic; D002454:Cell Differentiation; D016222:Fibroblast Growth Factor 2 / Q000378:metabolism; D017403:In Situ Hybridization; D051379:Mice; D008810:Mice, Inbred C57BL; D010006:Osteoblasts / Q000166:cytology / Q000378:metabolism; D010012:Osteogenesis; D012886:Skull / Q000196:embryology* / Q000378:metabolism*; D019063:Tenascin / Q000378:metabolism*
chemical_list: D019063:Tenascin; C477504:tenascin-N protein, mouse; D016222:Fibroblast Growth Factor 2