Immunohistochemical detection of dysbindin at the astroglial endfeet around the capillaries of mouse brain.
Dysbindin was first identified by the yeast two hybrid assay as a binding partner of dystrobrevin which is a cytoplasmic member of dystrophin glycoprotein complex. Immunolocalization of dystrobrevin in the astrocyte endfeet and endothelial cells in the rat cerebellum was reported. Therefore, we were interested in the expression and localization of dystrobrevin binding protein dysbindin in the mouse brain capillary wall and its surrounding astroglial endfeet. We examined whether the dysbindin expression is present in astroglial endfeet and/or capillary endothelial cells at light and electron microscopic levels. Using brain samples from five normal mice (C57BL/6ScSn), we prepared the anti-dysbindin antibody stained brain samples with immunoperoxidase method at light microscopic level and with immunogold method at ultrastructural level. Immunohistochemistry showed that dysbindin was located in the brain capillary at light microscopic level. Immunogold electron microscopy revealed that dysbindin signal was observed at the inside surface of plasma membrane of glial endfeet which surrounded the brain capillary endothelial cells and pericytes.
Iijima
Shoji
S
Department of Neurology, Showa University Fujigaoka Hospital, 1-30 Fujigaoka, Aoba-ku, Yokohama 227-8501, Japan.
Masaki
Hisatsugu
H
Wakayama
Yoshihiro
Y
Inoue
Masahiko
M
Jimi
Takahiro
T
Hara
Hajime
H
Unaki
Akihiko
A
Oniki
Hiroaki
H
Nakano
Kiyoko
K
Hirayama
Yoshiko
Y
Kishimoto
Koji
K
D000818:Animals; D001253:Astrocytes / Q000378:metabolism* / Q000648:ultrastructure; D001921:Brain / Q000378:metabolism* / Q000648:ultrastructure; D002196:Capillaries / Q000378:metabolism* / Q000648:ultrastructure; D002352:Carrier Proteins / Q000378:metabolism*; D000074765:Dysbindin; D049029:Dystrophin-Associated Proteins; D007150:Immunohistochemistry; D051379:Mice; D016253:Microscopy, Immunoelectron
D002352:Carrier Proteins; C498992:Dtnbp1 protein, mouse; D000074765:Dysbindin; D049029:Dystrophin-Associated Proteins