title: Biliary tract injury caused by different relative warm ischemia time in liver transplantation in rats.
abstract: BACKGROUND
  There is a controversy over the degree of liver and biliary injury caused by the period of secondary warm ischemia. A liver autotransplantation model was adopted because it excludes the effects of infection and immunological rejection on bile duct injury. This study was undertaken to assess biliary tract injury caused by relative warm ischemia (secondary warm ischemia time in the biliary tract) and reperfusion.
  
  
  METHODS
  One hundred and two rats were randomly divided into 5 groups: group I (control); groups II to V, relative warm ischemia times of 0 minute, 30 minutes, 1 hour and 2 hours. In addition to the levels of serum alkaline phosphatase, and total bilirubin, pathomorphology assessment and TUNEL assay were performed to evaluate biliary tract damage.
  
  
  RESULTS
  Under the conditions that there were no significant differences in warm ischemia time, cold perfusion time and anhepatic phase, group comparisons showed statistically significant differences. The least injury occurred in group II (portal vein and hepatic artery reperfused simultaneously) but the most severe injury occurred in group V (biliary tract relative warm ischemia time 2 hours).
  
  
  CONCLUSIONS
  Relative warm ischemia is one of the factors that result in bile duct injury, and the relationship between relative warm ischemia time the bile injury degree is time-dependent. Simultaneous arterial and portal reperfusion is the best choice to avoid the bile duct injury caused by relative warm ischemia.
authors.affiliation: Department of Hepatobiliary Surgery, Nanfang Hospital, Southern Medical University, Guangzhou, China.
authors.forename: Hong-Feng
authors.initials: HF
authors.lastname: Zhao
authors.forename: Guo-Wei
authors.initials: GW
authors.lastname: Zhang
authors.forename: Jie
authors.initials: J
authors.lastname: Zhou
authors.forename: Jian-Hua
authors.initials: JH
authors.lastname: Lin
authors.forename: Zhong-Lin
authors.initials: ZL
authors.lastname: Cui
authors.forename: Xiang-Hong
authors.initials: XH
authors.lastname: Li
mesh_terms: D000469:Alkaline Phosphatase / Q000097:blood; D000818:Animals; D017209:Apoptosis; D001652:Bile Ducts / Q000473:pathology / Q000503:physiopathology; D001659:Biliary Tract / Q000098:blood supply*; D001663:Bilirubin / Q000097:blood; D005260:Female; D006499:Hepatic Artery; D016031:Liver Transplantation; D008297:Male; D011169:Portal Vein; D051381:Rats; D017207:Rats, Sprague-Dawley; D015424:Reperfusion; D015427:Reperfusion Injury / Q000209:etiology*; D014182:Transplantation, Autologous; D052096:Warm Ischemia / Q000009:adverse effects*
chemical_list: D000469:Alkaline Phosphatase; D001663:Bilirubin