Stress-induced curcin-L promoter in leaves of Jatropha curcas L. and characterization in transgenic tobacco.
Ribosome-inactivating proteins (RIPs) represent a type of protein that universally inactivates the ribosome thus inhibiting protein biosynthesis. Curcin-L was a type I RIP found in Jatropha curcas L.. Its expression could be activated in leaves by treatments with abscisic acid, salicylic acid, polyethylene glycol, temperature 4, 45 degrees C and ultraviolet light. A 654 bp fragment of a 5' flanking region preceding the curcin-L gene, designated CP2, was cloned from the J. curcas genome and its expression pattern was studied via the expression of the beta-glucuronidase (GUS) gene in transgenic tobacco. Analysis of GUS activities showed that the CP2 was leaf specific, and was able to drive the expression of the reporter gene under stress-induction conditions. Analysis of a series of 5'-deletions of the CP2 suggested that several promoter motifs were necessary to respond to environmental stresses.
Qin
Xiaobo
X
College of Life Sciences, Sichuan University, 610064, Chengdu, People's Republic of China. qin_ever@hotmail.com
Zheng
Xiaojiang
X
Shao
Caixia
C
Gao
Jihai
J
Jiang
Luding
L
Zhu
Xunlu
X
Yan
Fang
F
Tang
Lin
L
Xu
Ying
Y
Chen
Fang
F
D000040:Abscisic Acid / Q000494:pharmacology; D005470:Fluorometry; D018506:Gene Expression Regulation, Plant / Q000187:drug effects / Q000235:genetics / Q000528:radiation effects; D031286:Jatropha / Q000187:drug effects / Q000235:genetics* / Q000528:radiation effects; D008969:Molecular Sequence Data; D018515:Plant Leaves / Q000235:genetics*; D010940:Plant Proteins / Q000235:genetics; D030821:Plants, Genetically Modified / Q000187:drug effects / Q000235:genetics* / Q000528:radiation effects; D011092:Polyethylene Glycols / Q000494:pharmacology; D011401:Promoter Regions, Genetic / Q000235:genetics*; D054789:Ribosome Inactivating Proteins, Type 1 / Q000235:genetics*; D020156:Salicylic Acid / Q000494:pharmacology; D013312:Stress, Physiological; D013696:Temperature; D014026:Nicotiana / Q000187:drug effects / Q000235:genetics* / Q000528:radiation effects; D014466:Ultraviolet Rays
D010940:Plant Proteins; D054789:Ribosome Inactivating Proteins, Type 1; D011092:Polyethylene Glycols; D000040:Abscisic Acid; D020156:Salicylic Acid