Microfluidic chips designed for measuring biomolecules through a microbead-based quantum dot fluorescence assay.
This chapter introduces the demonstration of specific antibody detection by using a microbead-based assay with quantum dot (QD) fluorescence on a polydimethylsiloxane (PDMS) microfluidic chip. The microfluidic chip is designed to isolate a single microbead where the binding reaction of antibodies occurs on the surface. The microfluidic chip is fabricated on a glass substrate using a transparent silicone elastomer, PDMS, for easy access of monitoring and flexible gate operations to capture the single microbead. For antibody detection, a sequence of functionalized assays has been performed in the fabricated chip, including the capturing of microbeads, antibody injection into a microchamber, quantum dot injection, and fluorescence detection. Various concentrations of human IgG antibodies have been introduced to bind to a single microbead captured and isolated inside a designated microchamber in a small volume of 75 pL. Fluorescence detection is monitored using a CCD camera after the second binding with the QDs conjugated with anti-human IgG. In this experiment, a human IgG antibody concentration below 0.1 microg/mL has been successfully detected.
Yun
Kwang-Seok
KS
Department of Electronic Engineering, Sogang University, Seoul, Mapo-gu, Korea. ksyun@sogang.ac.kr
Lee
Dohoon
D
Kim
Hak-Sung
HS
Yoon
Euisik
E
D000888:Antibodies, Anti-Idiotypic; D004129:Dimethylpolysiloxanes; D004867:Equipment Design; D005453:Fluorescence; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation* / Q000379:methods; D011506:Proteins / Q000032:analysis; D045663:Quantum Dots
D000888:Antibodies, Anti-Idiotypic; D004129:Dimethylpolysiloxanes; D007074:Immunoglobulin G; D011506:Proteins; C013830:baysilon