title: [Preparation and identification of the rat antibody against recombinant buckwheat trypsin inhibitor].
abstract: OBJECTIVE
  To prepare the rat antibody against the recombinant buckwheat trypsin inhibitor.
  
  
  METHODS
  The recombinant buckwheat trypsin inhibitor was expressed in E.coli. BL21 (DE3) and used as an immunogen to immunize the rat. The titer and specificity of the anti-BTI antibody from the rat were analyzed by ELISA, Western blot and immunohistochemistry, respectively.
  
  
  RESULTS
  ELISA detection indicated the titer of the antiserum was about 1:128 000. Western blot analysis showed the antibody reacted specifically with rBTI. The immunohistochemistry analysis proved that rBTI was expressed in the cytoplast and nucleus of EC9706 cells.
  
  
  CONCLUSIONS
  The rat antibody against rBTI has high titer and specificity, which is beneficial to further study on the molecular mechanisms in rBTI-induced apoptosis of tumor cells and also provide an important theory basis for further exploring the relationship between the structure and function of BTI.
authors.affiliation: Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Institute of Biotechnology, Shanxi University, Taiyuan 030006, China.
authors.forename: Yu-ying
authors.initials: YY
authors.lastname: Li
authors.forename: Xiao-dong
authors.initials: XD
authors.lastname: Cui
authors.forename: Zheng
authors.initials: Z
authors.lastname: Zhang
authors.forename: Zhuan-hua
authors.initials: ZH
authors.lastname: Wang
mesh_terms: D000818:Animals; D000906:Antibodies / Q000276:immunology*; D015153:Blotting, Western; D045744:Cell Line, Tumor; D002467:Cell Nucleus / Q000378:metabolism; D003593:Cytoplasm / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D019612:Fagopyrum / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D007106:Immune Sera / Q000276:immunology; D007150:Immunohistochemistry; D010940:Plant Proteins / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D051381:Rats; D011994:Recombinant Proteins / Q000276:immunology / Q000378:metabolism; D014361:Trypsin Inhibitors / Q000235:genetics / Q000276:immunology* / Q000378:metabolism
chemical_list: D000906:Antibodies; D007106:Immune Sera; D010940:Plant Proteins; D011994:Recombinant Proteins; D014361:Trypsin Inhibitors