Lens transplantation in zebrafish and its application in the analysis of eye mutants.
The lens plays an important role in the development of the optic cup. Using the zebrafish as a model organism, questions regarding lens development can be addressed. The zebrafish is useful for genetic studies due to several advantageous characteristics, including small size, high fecundity, short lifecycle, and ease of care. Lens development occurs rapidly in zebrafish. By 72 hpf, the zebrafish lens is functionally mature. Abundant genetic and molecular resources are available to support research in zebrafish. In addition, the similarity of the zebrafish eye to those of other vertebrates provides basis for its use as an excellent animal model of human defects. Several zebrafish mutants exhibit lens abnormalities, including high levels of cell death, which in some cases leads to a complete degeneration of lens tissues. To determine whether lens abnormalities are due to intrinsic causes or to defective interactions with the surrounding tissues, transplantation of a mutant lens into a wild-type eye is performed. Using fire-polished metal needles, mutant or wild-type lenses are carefully dissected from the donor animal, and transferred into the host. To distinguish wild-type and mutant tissues, a transgenic line is used as the donor. This line expresses membrane-bound GFP in all tissues, including the lens. This transplantation technique is an essential tool in the studies of zebrafish lens mutants.
Zhang
Yan
Y
The Second Teaching Hospital of Jilin University.
McCulloch
Kyle
K
Malicki
Jarema
J
D000818:Animals; D005260:Female; D007908:Lens, Crystalline / Q000637:transplantation*; D008297:Male; D015027:Zebrafish / Q000196:embryology / Q000235:genetics* / Q000601:surgery*