Cartilage regeneration of adipose-derived stem cells in a hybrid scaffold from fibrin-modified PLGA.
Adipose-derived stem cells (ASCs) appear to be a useful stem cell population, which has been shown to possess multipotentiality. The aim of this study was to evaluate the utility of ASCs in tissue-engineered cartilage using a hybrid scaffold from fibrin-modified PLGA scaffold. ASCs were isolated from rabbit adipose tissue. The PLGA scaffold was prepared by low-temperature deposition technology and the hybrid scaffold was fabricated by a freeze-drying method. When ASCs were seeded onto fibrin-modified PLGA scaffold in vitro, enhanced cellular viability was observed compared to unmodified PLGA scaffold. The analysis of proteoglycan and collagen II revealed that fibrin-modified scaffold succeeded in inducing ASCs to differentiate into chondrocytes in vitro. A preliminary study on cartilage regeneration was also performed in vivo. Observation of histology and immunoblotting demonstrated that ASCs containing the hybrid scaffold promoted cartilage regeneration in the defects of articular cartilage much better than other groups. These results indicated that ASCs containing the hybrid scaffold are a more effective way to potentially enhance articular cartilage regeneration.
Wei
Yiyong
Y
Shanghai Institute of Traumatology and Orthopaedics, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, People's Republic of China. yiyong_wei@hotmail.com
Hu
Hunyu
H
Wang
Haiqiang
H
Wu
Yinsong
Y
Deng
Lianfu
L
Qi
Jin
J
D000273:Adipose Tissue / Q000166:cytology*; D000818:Animals; D002358:Cartilage, Articular / Q000166:cytology / Q000502:physiology*; D002454:Cell Differentiation; D002470:Cell Survival; D019902:Chondrocytes / Q000166:cytology; D024043:Collagen Type II / Q000378:metabolism; D005337:Fibrin; D019344:Lactic Acid; D011100:Polyglycolic Acid; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011509:Proteoglycans / Q000378:metabolism; D011817:Rabbits; D012038:Regeneration; D054457:Tissue Scaffolds
D024043:Collagen Type II; D011509:Proteoglycans; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; D005337:Fibrin