Non-invasive method for evaluation of gastrocecal transit time by using a breath test in conscious rats.
The aim of this study was to establish a method for evaluating gastrocecal transit time using a simple and noninvasive 13C breath-test system in conscious rats. After fasting, rats were orally administered lactose-[13C]ureide. 13CO2 level in the expired air was measured at appropriate intervals. It was confirmed that priming dose of lactose-ureide was necessary for determining gastrocecal transit time. The level of 13CO2 in the expired air increased in a lactose-[13C]ureide dose-dependent manner. The gastrocecal transit time determined with 30 mg/kg of lactose-[13C]ureide was 160+/-24.5 min. This method is expected to contribute to the further understanding of intestinal pathophysiology.
Uchida
Masayuki
M
Food Science Institute, Division of Research and Development, Meiji Dairies Corporation, Kanagawa, Japan. masayuki_uchida@meiji-milk.com
Yoshida
Kaori
K
D000284:Administration, Oral; D000818:Animals; D001944:Breath Tests / Q000379:methods; D002245:Carbon Dioxide / Q000032:analysis*; D002247:Carbon Isotopes; D004305:Dose-Response Relationship, Drug; D005772:Gastrointestinal Transit; D007785:Lactose / Q000008:administration & dosage; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D014508:Urea / Q000008:administration & dosage / Q000031:analogs & derivatives*
D002247:Carbon Isotopes; D002245:Carbon Dioxide; C121442:lactose ureide; D014508:Urea; D007785:Lactose