title: Structural characteristics of the beta-sheet-like human and rat islet amyloid polypeptides as determined by scanning tunneling microscopy.
abstract: We demonstrate in this work that scanning tunneling microscopy (STM) provides a useful approach to obtaining structural information about human islet amyloid polypeptide (hIAPP) and rat islet amyloid polypeptide (rIAPP) assembly on highly oriented pyrolytic graphite (HOPG) with sub-molecular resolution. The observed hIAPP and rIAPP lamellae consisted of parallel stripes. The STM images of hIAPPs show multiple molecular folding structures, with an average of 11 amino acid residues for the core regions. In addition, the STM images also reveal the assembly characteristics of rIAPP lamellae and may indicate a secondary structural conformation from random coil to beta-sheet-like on the graphite surface.
authors.affiliation: National Center for Nanoscience and Technology, Beijing 100190, China.
authors.forename: Xiaobo
authors.initials: X
authors.lastname: Mao
authors.forename: Xiaojing
authors.initials: X
authors.lastname: Ma
authors.forename: Lei
authors.initials: L
authors.lastname: Liu
authors.forename: Lin
authors.initials: L
authors.lastname: Niu
authors.forename: Yanlian
authors.initials: Y
authors.lastname: Yang
authors.forename: Chen
authors.initials: C
authors.lastname: Wang
mesh_terms: D000682:Amyloid / Q000737:chemistry*; D000818:Animals; D006108:Graphite / Q000737:chemistry; D006801:Humans; D058228:Islet Amyloid Polypeptide; D018625:Microscopy, Atomic Force; D016252:Microscopy, Scanning Tunneling; D017433:Protein Structure, Secondary; D051381:Rats
chemical_list: D000682:Amyloid; D058228:Islet Amyloid Polypeptide; D006108:Graphite