title: Molecular characterization and kinetic properties of a novel two-domain taurocyamine kinase from the lung fluke Paragonimus westermani.
abstract: Taurocyamine kinase (TK) was previously reported to be restricted to certain marine annelids; however, the present study has proven otherwise. The lung fluke Paragonimus westermani has a contiguous two-domain TK with a mass of 80216 Da consisting of 713 amino acid residues sharing higher sequence identity with molluscan arginine kinase (AK). Both domains of P. westermani TK have significant activity for the substrate taurocyamine and exhibited synergism during substrate binding. Since TK plays a key role in energy metabolism and is not present in mammals, inhibitors against P. westermani TK could be effective novel chemotherapeutic agents and could be utilized for the development of specific diagnostic tools for the detection of paragonimiasis.
authors.affiliation: Department of Environmental Health Sciences, Kochi Medical School, Oko, Nankoku City, Kochi, Japan.
authors.forename: Blanca R
authors.initials: BR
authors.lastname: Jarilla
authors.forename: Shinji
authors.initials: S
authors.lastname: Tokuhiro
authors.forename: Mitsuru
authors.initials: M
authors.lastname: Nagataki
authors.forename: Sung-Jong
authors.initials: SJ
authors.lastname: Hong
authors.forename: Kouji
authors.initials: K
authors.lastname: Uda
authors.forename: Tomohiko
authors.initials: T
authors.lastname: Suzuki
authors.forename: Takeshi
authors.initials: T
authors.lastname: Agatsuma
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D015801:Helminth Proteins / Q000737:chemistry* / Q000378:metabolism; D007700:Kinetics; D008969:Molecular Sequence Data; D048492:Paragonimus westermani / Q000201:enzymology* / Q000378:metabolism; D017852:Phosphotransferases (Nitrogenous Group Acceptor) / Q000737:chemistry* / Q000378:metabolism; D010802:Phylogeny; D016415:Sequence Alignment
chemical_list: D015801:Helminth Proteins; D017852:Phosphotransferases (Nitrogenous Group Acceptor); C022716:taurocyamine kinase