title: Use of orbital shaken disposable bioreactors for mammalian cell cultures from the milliliter-scale to the 1,000-liter scale.
abstract: Driven by the commercial success of recombinant biopharmaceuticals, there is an increasing demand for novel mammalian cell culture bioreactor systems for the rapid production of biologicals that require mammalian protein processing. Recently, orbitally shaken bioreactors at scales from 50 mL to 1,000 L have been explored for the cultivation of mammalian cells and are considered to be attractive alternatives to conventional stirred-tank bioreactors because of increased flexibility and reduced costs. Adequate oxygen transfer capacity was maintained during the scale-up, and strategies to increase further oxygen transfer rates (OTR) were explored, while maintaining favorable mixing parameters and low-stress conditions for sensitive lipid membrane-enclosed cells. Investigations from process development to the engineering properties of shaken bioreactors are underway, but the feasibility of establishing a robust, standardized, and transferable technical platform for mammalian cell culture based on orbital shaking and disposable materials has been established with further optimizations and studies ongoing.
authors.affiliation: Laboratory of Cellular Biotechnology, Ecole Polytechnique Fédérale de Lausanne, CH-1015, Lausanne, Switzerland.
authors.forename: Xiaowei
authors.initials: X
authors.lastname: Zhang
authors.forename: Matthieu
authors.initials: M
authors.lastname: Stettler
authors.forename: Dario
authors.initials: D
authors.lastname: De Sanctis
authors.forename: Marco
authors.initials: M
authors.lastname: Perrone
authors.forename: Nicola
authors.initials: N
authors.lastname: Parolini
authors.forename: Marco
authors.initials: M
authors.lastname: Discacciati
authors.forename: Maria
authors.initials: M
authors.lastname: De Jesus
authors.forename: David
authors.initials: D
authors.lastname: Hacker
authors.forename: Alfio
authors.initials: A
authors.lastname: Quarteroni
authors.forename: Florian
authors.initials: F
authors.lastname: Wurm
mesh_terms: D000818:Animals; D001688:Biological Products / Q000378:metabolism / Q000592:standards*; D019149:Bioreactors; D001709:Biotechnology / Q000295:instrumentation* / Q000639:trends; D016466:CHO Cells; D002452:Cell Count; D018929:Cell Culture Techniques / Q000295:instrumentation*; D003412:Cricetulus; D004209:Disposable Equipment; D004867:Equipment Design; D006801:Humans; D007700:Kinetics; D010100:Oxygen / Q000378:metabolism; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000592:standards*; D014732:Vibration
chemical_list: D001688:Biological Products; D011994:Recombinant Proteins; D010100:Oxygen