title: Effect of radiofrequency electromagnetic field exposure on in vitro models of neurodegenerative disease.
abstract: In this work we tested viability, proliferation, and vulnerability of neural cells, after continuous radiofrequency (RF) electromagnetic fields exposure (global system for mobile telecommunications (GSM) modulated 900 MHz signal at a specific absorption rate (SAR) of 1 W/kg and maximum duration 144 h) generated by transverse electromagnetic cells. We used two cellular systems, SN56 cholinergic for example, SN56 cholinergic cell line and rat primary cortical neurons, and well-known neurotoxic challenges, such as glutamate, 25-35AA beta-amyloid, and hydrogen peroxide. Exposure to RF did not change viability/proliferation rate of the SN56 cholinergic cells or viability of cortical neurons. Co-exposure to RF exacerbated neurotoxic effect of hydrogen peroxide in SN56, but not in primary cortical neurons, whereas no cooperative effects of RF with glutamate and 25-35AA beta-amyloid were found. These data suggest that only under particular circumstances exposure to GSM modulated, 900 MHz signal act as a co-stressor for oxidative damage of neural cells.
authors.affiliation: Department of Veterinary Morphophysiology and Animal Production, Bologna University, Bologna, Italy.
authors.forename: Giovanna
authors.initials: G
authors.lastname: Del Vecchio
authors.forename: Alessandro
authors.initials: A
authors.lastname: Giuliani
authors.forename: Mercedes
authors.initials: M
authors.lastname: Fernandez
authors.forename: Pietro
authors.initials: P
authors.lastname: Mesirca
authors.forename: Ferdinando
authors.initials: F
authors.lastname: Bersani
authors.forename: Rosanna
authors.initials: R
authors.lastname: Pinto
authors.forename: Lucia
authors.initials: L
authors.lastname: Ardoino
authors.forename: Giorgio A
authors.initials: GA
authors.lastname: Lovisolo
authors.forename: Luciana
authors.initials: L
authors.lastname: Giardino
authors.forename: Laura
authors.initials: L
authors.lastname: Calzà
mesh_terms: D000818:Animals; D002470:Cell Survival / Q000187:drug effects*; D002478:Cells, Cultured; D004781:Environmental Exposure; D018698:Glutamic Acid / Q000378:metabolism*; D008872:Microwaves; D019636:Neurodegenerative Diseases / Q000503:physiopathology*; D009474:Neurons / Q000528:radiation effects*; D051381:Rats
chemical_list: D018698:Glutamic Acid