title: Saccharothrix violaceirubra sp. nov., isolated from soil and plant litter.
abstract: Two strains of nocardioform actinomycetes, isolated from soil and plant litter in Yamanashi prefecture, Japan, showed substrate mycelium with purple to dark-red colours. 16S rRNA gene sequence analysis indicated that the organisms belonged to the family Actinosynnemataceae and were related closely to Saccharothrix strains (96.7-98.0 % sequence similarity). The isolates contained MK-9(H(4)) as the predominant menaquinone, meso-diaminopimelic acid, galactose as the diagnostic whole-cell sugar and phosphatidylethanolamine as the predominant polar lipid. Based on a combination of cultural, physiological and chemotaxonomic properties, in addition to the phylogenetic analysis and DNA-DNA hybridization data, we propose a novel species, Saccharothrix violaceirubra sp. nov., for these strains, with the type strain YU 692-1(T) (=NBRC 102064(T) =KCTC 19326(T)).
authors.affiliation: NITE Biological Resource Center (NBRC), Department of Biotechnology, National Institute of Technology and Evaluation (NITE), 2-5-8 Kazusakamatari, Kisarazu, Chiba 292-0818, Japan. otoguro-misa@nite.go.jp
authors.forename: Misa
authors.initials: M
authors.lastname: Otoguro
authors.forename: Tomohiko
authors.initials: T
authors.lastname: Tamura
authors.forename: Ken-Ichiro
authors.initials: K
authors.lastname: Suzuki
authors.forename: Masayuki
authors.initials: M
authors.lastname: Hayakawa
mesh_terms: D000192:Actinomycetales / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology; D015373:Bacterial Typing Techniques; D004269:DNA, Bacterial / Q000032:analysis; D004275:DNA, Ribosomal / Q000032:analysis; D005227:Fatty Acids / Q000032:analysis; D007564:Japan; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010641:Phenotype; D010802:Phylogeny; D010944:Plants / Q000378:metabolism; D016133:Polymerase Chain Reaction / Q000379:methods; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D013045:Species Specificity
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S