title: Determining the stoichiometry and binding constants of inclusion complexes formed between aromatic compounds and beta-cyclodextrin by solid-phase microextraction coupled to high-performance liquid chromatography.
abstract: The complexation of native beta-cyclodextrin (CD) and seven aromatic compounds, namely, phenetole, toluene, m-xylene, naphthalene, biphenyl, fluorene and phenanthrene, has been studied for first time utilizing a solid-phase microextraction (SPME)-high-performance liquid chromatography (HPLC) method. The stoichiometries of the analyte:beta-CD complexes were found to be either 1:1 or 1:2. The formation of 1:2 complexes was confirmed for naphthalene, biphenyl, fluorene, and phenanthrene only when utilizing relatively high concentrations of beta-CD (up to 6.6 mM). The 1:2 stoichiometries were confirmed using the classical modified Benesi-Hildebrand (BH) method. The calculated binding constants for 1:1 stoichiometries (K(1)) using the SPME method varied from 115.3M(-1) for toluene to 3510 M(-1) for phenanthrene, whereas the corresponding values to the 1:2 stoichiometries (K(3)) varied from 7.30 x 10(5) M(-2) for biphenyl to 9.03 x 10(6)M(-2) for naphthalene.
authors.affiliation: Department of Chemistry, The University of Toledo, Toledo, OH 43606, USA.
authors.forename: Guillaume
authors.initials: G
authors.lastname: Chalumot
authors.forename: Cong
authors.initials: C
authors.lastname: Yao
authors.forename: Verónica
authors.initials: V
authors.lastname: Pino
authors.forename: Jared L
authors.initials: JL
authors.lastname: Anderson
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006841:Hydrocarbons, Aromatic / Q000737:chemistry*; D007700:Kinetics; D052617:Solid Phase Microextraction / Q000379:methods*; D047392:beta-Cyclodextrins / Q000737:chemistry*
chemical_list: D006841:Hydrocarbons, Aromatic; D047392:beta-Cyclodextrins; C031215:betadex