Use of the DNA Checkerboard hybridization method for detection and quantitation of Candida species in oral microbiota.
The DNA Checkerboard method enables the simultaneous identification of distinct microorganisms in a large number of samples and employs up to 45 whole genomic DNA probes to gram-negative and gram-positive bacterial species present in subgingival biofilms. Collectively, they account for 55%-60% of the bacteria in subgingival biofilms. In this study, we present the DNA Checkerboard hybridization as an alternative method for the detection and quantitation of Candida species in oral cavities. Our results reveal that DNA Checkerboard is sensitive enough and constitutes a powerful and appropriate method for detecting and quantifying Candida species found in the oral cavity.
do Nascimento
Cássio
C
Ribeirão Preto Dental School, University of São Paulo, Department of Dental Materials and Prosthodontics, Avenida do Café S/N, Monte Alegre, Ribeirão Preto, São Paulo, Brazil.
Ferreira de Albuquerque Junior
Rubens
R
Issa
João Paulo Mardegan
JP
Ito
Izabel Yoko
IY
Lovato da Silva
Cláudia Helena
CH
de Freitas Oliveira Paranhos
Helena
H
de Souza
Raphael Freitas
RF
D000368:Aged; D000369:Aged, 80 and over; D018441:Biofilms; D002175:Candida / Q000235:genetics* / Q000302:isolation & purification*; D002177:Candidiasis / Q000382:microbiology*; D015169:Colony Count, Microbial / Q000379:methods*; D004271:DNA, Fungal / Q000235:genetics*; D005260:Female; D005881:Gingiva / Q000382:microbiology; D006801:Humans; D008297:Male; D009693:Nucleic Acid Hybridization / Q000379:methods*; D012680:Sensitivity and Specificity
D004271:DNA, Fungal