title: Vaccination of dogs with six different candidate leishmaniasis vaccines composed of a chimerical recombinant protein containing ribosomal and histone protein epitopes in combination with different adjuvants.
abstract: Chimerical protein "Q", composed of antigenic ribosomal and histone sequences, in combination with live BCG is a promising canine leishmaniasis vaccine candidate; one of the few vaccine candidates that have been tested successfully in dogs. Unfortunately, live BCG is not an appropriate adjuvant for commercial application due to safety problems in dogs. In order to find a safe adjuvant with similar efficacy to live BCG, muramyl dipeptide, aluminium hydroxide, Matrix C and killed Propionibacterium acnes in combination with either E. coli- or baculovirus-produced recombinant JPCM5_Q protein were tested. Groups of five or seven dogs were vaccinated with six different adjuvant-antigen combinations and challenged with a high dose intravenous injection of Leishmania infantum JPC strain promastigotes. All candidate vaccines proved to be safe, and both humoral and cellular responses to the recombinant proteins were detected at the end of the prime-boost vaccination scheme. However, clinical and parasitological data obtained during the 10 month follow-up period indicated that protection was not induced by either of the six candidate vaccines. Although no direct evidence was obtained, our data suggest that live BCG may have a significant protective effect against challenge with L. infantum in dogs.
authors.affiliation: Intervet International B.V., Wim de Körverstraat 35, 5831AN Boxmeer, the Netherlands. jacqueline.poot@sp.intervet.com
authors.forename: J
authors.initials: J
authors.lastname: Poot
authors.forename: L H M
authors.initials: LH
authors.lastname: Janssen
authors.forename: T J
authors.initials: TJ
authors.lastname: van Kasteren-Westerneng
authors.forename: K H A
authors.initials: KH
authors.lastname: van der Heijden-Liefkens
authors.forename: V E J C
authors.initials: VE
authors.lastname: Schijns
authors.forename: A
authors.initials: A
authors.lastname: Heckeroth
mesh_terms: D000276:Adjuvants, Immunologic; D000595:Amino Acid Sequence; D000818:Animals; D000913:Antibodies, Protozoan / Q000276:immunology; D000953:Antigens, Protozoan / Q000276:immunology*; D001483:Base Sequence; D049109:Cell Proliferation; D004283:Dog Diseases / Q000276:immunology / Q000517:prevention & control*; D004285:Dogs; D000939:Epitopes / Q000276:immunology; D005260:Female; D006657:Histones / Q000276:immunology*; D006968:Hypersensitivity, Delayed / Q000276:immunology; D018314:Leishmania infantum / Q000276:immunology; D054332:Leishmaniasis Vaccines / Q000276:immunology*; D007898:Leishmaniasis, Visceral / Q000276:immunology / Q000517:prevention & control / Q000662:veterinary*; D008213:Lymphocyte Activation / Q000276:immunology; D008297:Male; D008969:Molecular Sequence Data; D009163:Mycobacterium bovis / Q000276:immunology; D011994:Recombinant Proteins / Q000276:immunology; D012269:Ribosomal Proteins / Q000276:immunology*
chemical_list: D000276:Adjuvants, Immunologic; D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D000939:Epitopes; D006657:Histones; D054332:Leishmaniasis Vaccines; D011994:Recombinant Proteins; D012269:Ribosomal Proteins