Antitumorigenic effect of brain proline rich polypeptide-1 in human chondrosarcoma.
Proline rich polypeptide (PRP-1) produced by neurosecretory cells of the hypothalamus is one of the fragments of neurophysin-vasopressin-associated glycoprotein. The primary structure of the neuropeptide PRP-1 isolated from neurosecretory granules of bovine neurohypophysis. We investigated PRP-1 action on chondrosarcoma, the second most common malignancy in bone, which primarily affects the cartilage cells. This deadly disease does not have any effective treatment. Earlier we demonstrated MYC oncogene inactivating effect by 1 lg/ml concentration brain PRP-1 In the present study we observed reduced viable sarcoma JJ012 cell numbers in comparison with control (89% growth inhibition) when treated with low concentrations of PRP-1 (0.5–1 lg/ml). Higher concentrations did not exhibit inhibitory effect. We assume that PRP-1 in low concentration impedes cell cycle progression. The fact that low concentrations of PRP-1 abolished Myc activity prompts to think that the antitumorigenic effect of PRP-1 in low concentrations is mediated through oncogene inactivation.
Galoian
Karina
K
Department of Orthopedics, UHealth, Miller School of Medicine, University of Miami, 1600 NW 10th Ave, Suite 8006 (r-2), Miami, FL 33136, USA. kgaloian@med.miami.edu
Scully
Sean
S
McNamara
George
G
Flynn
Patrick
P
Galoyan
Armen
A
D023181:Antimicrobial Cationic Peptides; D001859:Bone Neoplasms / Q000188:drug therapy*; D045744:Cell Line, Tumor; D002813:Chondrosarcoma / Q000188:drug therapy*; D016259:Genes, myc / Q000187:drug effects; D006801:Humans; D010455:Peptides / Q000627:therapeutic use*
D023181:Antimicrobial Cationic Peptides; C540268:PRP-1 peptide; D010455:Peptides