Species-specific detection of Listeria monocytogenes using polymerase chain reaction assays targeting the prfA virulence gene cluster.
Sequence comparison of individual genes in the prfA virulence gene cluster (pVGC), a central virulence gene cluster, from different Listeria species indicated that priming sites within the genes appeared to be specific for Listeria monocytogenes exclusively. Therefore, the pVGC was targeted for polymerase chain reaction (PCR) assays to detect and specifically identify L. monocytogenes. Each gene of the pVGC was specifically amplified in L. monocytogenes but not in other Listeria species.
Jung
Hye-Jin
HJ
Department of Food Science and Technology, College of Industrial Sciences, Chung-Ang University, Ansung, Kyungki-do, Korea.
Park
Sung-Hee
SH
Ha
Sang-Do
SD
Lee
Kyu-Ho
KH
Chung
Duck Hwa
DH
Kim
Cheol-Ho
CH
Kim
Min-Gon
MG
Kim
Kwang-Yup
KY
Kim
Keun-Sung
KS
D001483:Base Sequence; D017931:DNA Primers; D005798:Genes, Bacterial; D008089:Listeria monocytogenes / Q000235:genetics / Q000472:pathogenicity*; D005810:Multigene Family; D016133:Polymerase Chain Reaction / Q000379:methods*; D013045:Species Specificity; D014774:Virulence / Q000235:genetics*
D017931:DNA Primers