Glucosinolate engineering identifies a gamma-glutamyl peptidase.
Consumption of cruciferous vegetables is associated with reduced risk of developing cancer, a phenomenon attributed to glucosinolates, which are characteristic of these vegetables. We report production of the bioactive benzylglucosinolate in the noncruciferous plant Nicotiana benthamiana through metabolic engineering. The study includes identification of gamma-glutamyl peptidase 1 (GGP1), which substantially increased glucosinolate production by metabolizing an accumulating glutathione conjugate, an activity not previously described for glucosinolate biosynthesis or for proteins containing glutamine amidotransferase domains.
Geu-Flores
Fernando
F
Plant Biochemistry Laboratory, Department of Plant Biology, Faculty of Life Sciences, University of Copenhagen, Frederiksberg, Denmark.
Nielsen
Morten Thrane
MT
Nafisi
Majse
M
Møldrup
Morten Emil
ME
Olsen
Carl Erik
CE
Motawia
Mohammed Saddik
MS
Halkier
Barbara Ann
BA
D005961:Glucosinolates / Q000096:biosynthesis*; D016366:Open Reading Frames; D018515:Plant Leaves / Q000201:enzymology / Q000235:genetics / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics*; D030821:Plants, Genetically Modified / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism; D013861:Thiocyanates; D013863:Thioglucosides / Q000096:biosynthesis*; D014026:Nicotiana / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism; D014170:Transformation, Genetic
D005961:Glucosinolates; D010940:Plant Proteins; D013861:Thiocyanates; D013863:Thioglucosides; C408882:benzylglucosinolic acid