Near infrared sensing based on fluorescence resonance energy transfer between Mn:CdTe quantum dots and Au nanorods.
A novel sensing system based on the near infrared (NIR) fluorescence resonance energy transfer (FRET) between Mn:CdTe quantum dots (Qdots) and Au nanorods (AuNRs) was established for the detection of human IgG. The NIR-emitting Qdots linked with goat anti-human IgG (Mn:CdTe-Ab1) and AuNRs linked with rabbit anti-human IgG (AuNRs-Ab2) acted as fluorescence donors and acceptors, respectively. FRET occurred by human IgG with the specific antigen-antibody interaction. And human IgG was detected based on the modulation in FRET efficiency. The calibration graph was linear over the range of 0.05-2.5 microM of human IgG under optimal conditions. The proposed sensing system can decrease the interference of biomolecules in NIR region and increase FRET efficiency in optimizing the spectral overlap of AuNRs with Mn:CdTe Qdots. This method has great potential for multiplex assay with different donor-acceptor pairs.
Liang
Guo-Xi
GX
Key Lab. of Analytical Chemistry for Life Science (MOE), School of Chemistry and Chemical Engineering, Nanjing University, Hankou Road 22, Nanjing 210093, PR China.
Pan
Hong-Cheng
HC
Li
Ye
Y
Jiang
Li-Ping
LP
Zhang
Jian-Rong
JR
Zhu
Jun-Jie
JJ
D015374:Biosensing Techniques / Q000295:instrumentation*; D019187:Cadmium Compounds / Q000737:chemistry*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D031541:Fluorescence Resonance Energy Transfer / Q000295:instrumentation*; D006046:Gold / Q000737:chemistry*; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis; D008345:Manganese / Q000737:chemistry; D036103:Nanotechnology / Q000295:instrumentation; D043942:Nanotubes / Q000737:chemistry* / Q000648:ultrastructure; D045663:Quantum Dots; D019265:Spectroscopy, Near-Infrared / Q000295:instrumentation*; D013691:Tellurium / Q000737:chemistry*
D019187:Cadmium Compounds; D007074:Immunoglobulin G; D008345:Manganese; D006046:Gold; D013691:Tellurium; C028337:cadmium telluride