Mandibular repair in rats with premineralized silk scaffolds and BMP-2-modified bMSCs.
Premineralized silk fibroin protein scaffolds (mSS) were prepared to combine the osteoconductive properties of biological apatite with aqueous-derived silk scaffold (SS) as a composite scaffold for bone regeneration. The aim of present study was to evaluate the effect of premineralized silk scaffolds combined with bone morphogenetic protein-2 (BMP-2) modified bone marrow stromal cells (bMSCs) to repair mandibular bony defects in a rat model. bMSCs were expanded and transduced with adenovirus AdBMP-2, AdLacZ gene in vitro. These genetically modified bMSCs were then combined with premineralized silk scaffolds to form tissue-engineered bone. Mandibular repairs with AdBMP-2 transduced bMSCs/mSS constructs were compared with those treated with AdLacZ-transduced bMSCs/mSS constructs, native (nontransduced) bMSCs/mSS constructs and mSS alone. Eight weeks after post-operation, the mandibles were explanted and evaluated by radiographic observation, micro-CT, histological analysis and immunohistochemistry. The presence of BMP-2 gene enhanced tissue-engineered bone in terms of the most new bone formed and the highest local bone mineral densities (BMD) found. These results demonstrated that premineralized silk scaffold could serve as a potential substrate for bMSCs to construct tissue-engineered bone for mandibular bony defects. BMP-2 gene therapy and tissue engineering techniques could be used in mandibular repair and bone regeneration.
Jiang
Xinquan
X
Oral Bioengineering Lab, Shanghai Research Institute of Stomatology, Ninth People's Hospital Affiliated to Shanghai Jiao Tong University, School of Medicine, Shanghai Key Laboratory of Stomatology, Shanghai 200011, China.
Zhao
Jun
J
Wang
Shaoyi
S
Sun
Xiaojuan
X
Zhang
Xiuli
X
Chen
Jake
J
Kaplan
David L
DL
Zhang
Zhiyuan
Z
D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D001854:Bone Marrow Cells / Q000166:cytology / Q000648:ultrastructure; D055396:Bone Morphogenetic Protein 2 / Q000235:genetics / Q000378:metabolism*; D001861:Bone Regeneration; D002113:Calcification, Physiologic; D002454:Cell Differentiation; D049109:Cell Proliferation; D007150:Immunohistochemistry; D020007:Implants, Experimental; D008334:Mandible / Q000000981:diagnostic imaging / Q000473:pathology* / Q000601:surgery; D010012:Osteogenesis / Q000235:genetics; D051381:Rats; D020133:Reverse Transcriptase Polymerase Chain Reaction; D047011:Silk / Q000378:metabolism*; D017154:Stromal Cells / Q000166:cytology / Q000378:metabolism* / Q000648:ultrastructure; D054457:Tissue Scaffolds / Q000737:chemistry*; D014161:Transduction, Genetic; D014945:Wound Healing; D055114:X-Ray Microtomography
D015415:Biomarkers; D055396:Bone Morphogenetic Protein 2; D047011:Silk