title: Characterization of marine microalga, Scenedesmus sp. strain JPCC GA0024 toward biofuel production.
abstract: A marine microalga, strain JPCC GA0024 was selected as high amount of neutral lipid producers from marine microalgal culture collection toward biofuel production. The strain was tentatively identified as Scenedesmus rubescens by 18S rDNA analysis. The growth of strain JPCC GA0024 was influenced by artificial seawater concentrations. The optimum growth of 0.79 g/l was obtained at 100% artificial seawater. The lipid accumulation reached 73.0% of dry cell weight at 100% artificial seawater without additional nutrients for 11 days. Gas chromatography/mass spectrometry analysis indicates that lipid fraction mainly contained hydrocarbons including mainly hexadecane (C(16) H(34)) and 1-docosene (C(22) H(44)). Furthermore, calorimetric analysis revealed that the energy content of strain JPCC GA0024 was 6,160 kcal/kg (25.8 MJ/kg) of calorific value, which was equivalent to the coal engery. The strain JPCC GA0024, S. rubescens, will become a promising resource that can grow as a dominant species in the seawater for the production of both liquid and solid biofuels.
authors.affiliation: Department of Biotechnology, Tokyo University of Agriculture and Technology, 2-24-16, Naka-cho, Koganei, Tokyo 184-8588, Japan. tmatsuna@cc.tuat.ac.jp
authors.forename: Tadashi
authors.initials: T
authors.lastname: Matsunaga
authors.forename: Mitsufumi
authors.initials: M
authors.lastname: Matsumoto
authors.forename: Yoshiaki
authors.initials: Y
authors.lastname: Maeda
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Sugiyama
authors.forename: Reiko
authors.initials: R
authors.lastname: Sato
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Tanaka
mesh_terms: D048028:DNA, Algal / Q000737:chemistry / Q000235:genetics; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D008401:Gas Chromatography-Mass Spectrometry; D020459:Genes, rRNA; D008055:Lipids / Q000032:analysis*; D010802:Phylogeny; D048030:RNA, Algal / Q000235:genetics; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012337:RNA, Ribosomal, 18S / Q000235:genetics; D044444:Scenedesmus / Q000737:chemistry / Q000254:growth & development / Q000302:isolation & purification / Q000378:metabolism*; D012623:Seawater / Q000382:microbiology; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid
chemical_list: D048028:DNA, Algal; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D008055:Lipids; D048030:RNA, Algal; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D012337:RNA, Ribosomal, 18S