Microfluidic ARray Synthesizer (MArS) for rapid preparation and hybridization of custom DNA microarray.
The purpose of this work is to demonstrate a simple method that integrates custom DNA array synthesis and hybridization in a microfluidic channel. Commercial DLP projectors and a classical oligonucleotide synthesizer were integrated with a microfluidic chamber and constitute a Microfluidic ARray Synthesizer (MArS). Utilizing the benefit of microfluidics, the MArS produces two custom oligonucleotide arrays with reagent consumption for one array. The quality of the two arrays was examined by hybridization and shown to be almost identical. Post-synthesis deprotection of the array and hybridization performed in the same microfluidic chamber reduced overall DNA assay time by two orders. In addition, mismatch discrimination was enhanced using the microfluidic chamber. In summary, a simple method to build a custom array synthesizer is demonstrated and its performance is examined.
Cheng
Ji-Yen
JY
Research Center for Applied Sciences, Academia Sinica Taiwan, 128 Sec.2 Academia Rd., Taipei City 11529, Taiwan. jycheng@gate.sinica.edu.tw
Chen
Huai-Yi
HY
D044085:Microfluidics / Q000379:methods*; D009693:Nucleic Acid Hybridization / Q000379:methods*; D020411:Oligonucleotide Array Sequence Analysis / Q000191:economics / Q000379:methods*; D013997:Time Factors