title: In silico analysis for identification of tick phagotopes selected by phage-displayed libraries.
abstract: Phage display techniques have been widely employed to map epitope structures which have served as the basis for developing molecular vaccines. We have applied this technique to map specific epitopes of Rhipicephalus (Boophilus) microplus. In the present study, we have identified the potential immunogens using a process in which the selected phage clones were analyzed through bioinformatics, prior to final field tests. The present study demonstrates the feasibility of identifying important R. (B.) microplus phagotopes for vaccine development through screening of phage-displayed random peptide libraries and bioinformatics tools.
authors.affiliation: Laboratório de Bionanotecnologia, Instituto de Genética e Bioquímica, Universidade Federal de Uberlândia--UFU, Brazil. crprudencio@gmail.com
authors.forename: Carlos Roberto
authors.initials: CR
authors.lastname: Prudencio
authors.forename: Rafael
authors.initials: R
authors.lastname: Nascimento
authors.forename: Moacir Marchiori
authors.initials: MM
authors.lastname: Filho
authors.forename: Andrea de Oliveira Marques
authors.initials: Ade O
authors.lastname: Marra
authors.forename: Guilherme Rocha Lino
authors.initials: GR
authors.lastname: de Souza
authors.forename: Juliana Franco
authors.initials: JF
authors.lastname: Almeida
authors.forename: Rone
authors.initials: R
authors.lastname: Cardoso
authors.forename: Matias Pablo Juan
authors.initials: MP
authors.lastname: Szabó
authors.forename: Luiz Ricardo
authors.initials: LR
authors.lastname: Goulart
mesh_terms: D000818:Animals; D019151:Peptide Library; D013987:Ticks / Q000145:classification*
chemical_list: D019151:Peptide Library