Brain extracellular matrix affects AMPA receptor lateral mobility and short-term synaptic plasticity.
Many synapses in the mature CNS are wrapped by a dense extracellular matrix (ECM). Using single-particle tracking and fluorescence recovery after photobleaching, we found that this net-like ECM formed surface compartments on rat primary neurons that acted as lateral diffusion barriers for AMPA-type glutamate receptors. Enzymatic removal of the ECM increased extrasynaptic receptor diffusion and the exchange of synaptic AMPA receptors. Whole-cell patch-clamp recording revealed an increased paired-pulse ratio as a functional consequence of ECM removal. These results suggest that the surface compartments formed by the ECM hinder lateral diffusion of AMPA receptors and may therefore modulate short-term synaptic plasticity.
Frischknecht
Renato
R
Leibniz Institute for Neurobiology, Magdeburg, Germany.
Heine
Martin
M
Perrais
David
D
Seidenbecher
Constanze I
CI
Choquet
Daniel
D
Gundelfinger
Eckart D
ED
D000818:Animals; D001921:Brain / Q000502:physiology*; D002478:Cells, Cultured; D003712:Dendrites / Q000502:physiology; D005109:Extracellular Matrix / Q000502:physiology*; D036681:Fluorescence Recovery After Photobleaching; D006821:Hyaluronoglucosaminidase / Q000378:metabolism; D066298:In Vitro Techniques; D051379:Mice; D009473:Neuronal Plasticity / Q000502:physiology*; D009474:Neurons / Q000502:physiology; D018408:Patch-Clamp Techniques; D051381:Rats; D017207:Rats, Sprague-Dawley; D018091:Receptors, AMPA / Q000378:metabolism*; D016194:Receptors, N-Methyl-D-Aspartate / Q000378:metabolism; D013569:Synapses / Q000502:physiology*; D009435:Synaptic Transmission / Q000502:physiology
D006821:Hyaluronoglucosaminidase; D018091:Receptors, AMPA; D016194:Receptors, N-Methyl-D-Aspartate; C409466:NR1 NMDA receptor; C120997:NR2A NMDA receptor; C104722:glutamate receptor ionotropic, AMPA 2