First isolation of Tenacibaculum maritimum from wedge sole, Dicologoglossa cuneata (Moreau).
The first isolation of Tenacibaculum maritimum from wedge sole, Dicologoglossa cuneata, is reported. The pathogen was recovered from ulcers of cultured fish, from three different outbreaks. The six isolates obtained were biochemically and serologically characterized and diagnosis was confirmed by polymerase chain reaction using specific primers and partial 16S rRNA gene sequencing. The isolates constituted a homogeneous phenotypic group; however, they belong to two of the different serotypes described within this species. A virulence evaluation of the isolates using Wedge sole fry was also performed.
López
J R
JR
IFAPA Centro Agua del Pino, Huelva, Spain.
Núñez
S
S
Magariños
B
B
Castro
N
N
Navas
J I
JI
de la Herran
R
R
Toranzo
A E
AE
D000818:Animals; D001483:Base Sequence; D016000:Cluster Analysis; D017931:DNA Primers / Q000235:genetics; D005412:Flatfishes / Q000382:microbiology*; D041965:Flavobacteriaceae / Q000235:genetics* / Q000302:isolation & purification* / Q000472:pathogenicity; D008969:Molecular Sequence Data; D010641:Phenotype; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012703:Serotyping; D014774:Virulence
D017931:DNA Primers; D012336:RNA, Ribosomal, 16S