title: Optical trapping and fluorescence excitation with violet diode lasers and extended cavity surface emitting lasers.
abstract: Violet diode lasers and vertical extended cavity surface emitting lasers are used within an optical trapping system. Two distinct but related studies are performed. Firstly, the optical trapping efficiency in terms of the Q parameter for micron and sub-micron sized particles is determined. Secondly, we use the violet diode laser to observe and fluoresce 4'-6- Diamidino-2-phenylindole (DAPI) stained chromosomes, green fluorescent protein (GFP) transfected neuroblastomas, and fluorescent polymer spheres within an optical tweezers using a 1064nm trap laser. This work paves the way for both reduced trap volumes and for biological tagging, chromosome selection, or observing protein dynamics.
authors.forename: Tanya
authors.initials: T
authors.lastname: Lake
authors.forename: Antonia
authors.initials: A
authors.lastname: Carruthers
authors.forename: Margaret
authors.initials: M
authors.lastname: Taylor
authors.forename: Lynn
authors.initials: L
authors.lastname: Paterson
authors.forename: Frank
authors.initials: F
authors.lastname: Gunn-Moore
authors.forename: John
authors.initials: J
authors.lastname: Allen
authors.forename: Wilson
authors.initials: W
authors.lastname: Sibbett
authors.forename: Kishan
authors.initials: K
authors.lastname: Dholakia