2-Deoxy-D-glucose, but not mercaptoacetate, increases food intake in decerebrate rats.
We examined food intake in chronically maintained decerebrate rats in response to two antimetabolic drugs known to stimulate food intake, 2-mercaptoacetate (MA) and 2-deoxy-D-glucose (2DG). MA reduces fatty acid oxidation, and 2DG reduces glucose utilization. Because previous work has shown that insulin-induced hypoglycemia increases food intake in decerebrate rats, we predicted that 2DG would have this same effect. MA-induced feeding requires vagal sensory neurons that terminate in the hindbrain. Cholecystokinin-induced suppression of feeding, which likewise requires vagal sensory neurons, has been shown to suppress food intake in decerebrate rats. Therefore, we predicted that MA's effects on feeding would also persist in decerebrate rats. In our experiments, the test diet (40% milk, diluted with water) was infused intraorally through a chronic cheek fistula. We found that sham controls consumed 258% and 230% of their baseline milk intake in response to 2DG and MA, respectively. Decerebrates consumed 239% of their baseline milk intake in response to 2DG, but did not increase their intake in response to MA. Because decerebration separates the hindbrain from the forebrain, these results indicate that 2DG-induced glucoprivation is capable of acting within the hindbrain to activate fundamental reflex circuitry for consummatory feeding responses, as shown previously for hypoglycemia. In contrast, MA affects food consumption only after forebrain processing of MA-induced vagal afferent signals and in the presence of intact ascending and descending neural pathways.
Darling
Rebecca A
RA
Programs in Neuroscience, Washington State Univ., Pullman, WA, USA.
Ritter
Sue
S
D000818:Animals; D000963:Antimetabolites / Q000494:pharmacology; D001069:Appetite Regulation / Q000502:physiology; D003655:Decerebrate State / Q000503:physiopathology*; D003847:Deoxyglucose / Q000494:pharmacology*; D004435:Eating / Q000187:drug effects*; D008297:Male; D016548:Prosencephalon / Q000601:surgery; D051381:Rats; D017207:Rats, Sprague-Dawley; D012249:Rhombencephalon / Q000601:surgery; D013864:Thioglycolates / Q000494:pharmacology
D000963:Antimetabolites; D013864:Thioglycolates; C017487:2-mercaptoacetate; D003847:Deoxyglucose