title: Species-specific detection of Listeria monocytogenes using polymerase chain reaction assays targeting the prfA virulence gene cluster.
abstract: Sequence comparison of individual genes in the prfA virulence gene cluster (pVGC), a central virulence gene cluster, from different Listeria species indicated that priming sites within the genes appeared to be specific for Listeria monocytogenes exclusively. Therefore, the pVGC was targeted for polymerase chain reaction (PCR) assays to detect and specifically identify L. monocytogenes. Each gene of the pVGC was specifically amplified in L. monocytogenes but not in other Listeria species.
authors.affiliation: Department of Food Science and Technology, College of Industrial Sciences, Chung-Ang University, Ansung, Kyungki-do, Korea.
authors.forename: Hye-Jin
authors.initials: HJ
authors.lastname: Jung
authors.forename: Sung-Hee
authors.initials: SH
authors.lastname: Park
authors.forename: Sang-Do
authors.initials: SD
authors.lastname: Ha
authors.forename: Kyu-Ho
authors.initials: KH
authors.lastname: Lee
authors.forename: Duck Hwa
authors.initials: DH
authors.lastname: Chung
authors.forename: Cheol-Ho
authors.initials: CH
authors.lastname: Kim
authors.forename: Min-Gon
authors.initials: MG
authors.lastname: Kim
authors.forename: Kwang-Yup
authors.initials: KY
authors.lastname: Kim
authors.forename: Keun-Sung
authors.initials: KS
authors.lastname: Kim
mesh_terms: D001483:Base Sequence; D017931:DNA Primers; D005798:Genes, Bacterial; D008089:Listeria monocytogenes / Q000235:genetics / Q000472:pathogenicity*; D005810:Multigene Family; D016133:Polymerase Chain Reaction / Q000379:methods*; D013045:Species Specificity; D014774:Virulence / Q000235:genetics*
chemical_list: D017931:DNA Primers