[Rebamipide inhibited expression of TLR4 and TNF-alpha release in pulmonary epithelial cell line A549 induced by lipopolysaccharide].
OBJECTIVE
To determine the effect of rebamipide on the expression of Toll-like recepter 4 (TLR4) and TNF-alpha release in pulmonary epithelial cell line A549.


METHODS
Lipopolysaccharide (LPS) was used to induce A549 in vitro, which was divided into 4 groups: a control group, a model group(LPS), and 2 intervention groups (10 mg/L rebamipide plus LPS; 30 mg/L rebamipide plus LPS). TNF-alpha release was detected with ELISA and expression of TLR4 was detected with RT-PCR and Western blot.


RESULTS
A549 cells were stimulated with LPS and TNF-alpha release was increased compared with the control group (P<0.01), peaking at 6 h. Expression of TLR4 was also increased compared with the control group (P<0.01), but it was inhibited by rebamipide compared with the model group (P<0.05). There was no significant difference between the 2 intervention groups (P>0.05).


CONCLUSIONS
The antiinflammatory mechanism of rebamipide may be reducing cytokine release by inhibiting TLR4 expression. Rebamipide may be used as a supplementary anti-infection drug.
Wen
Xiufang
X
Department of Respiratory Medicine, Third People's Hospital of Chongqing, Chongqing 400014, China wenranran221@126.com
Chen
Xia
X
Zhou
Xiangdong
X
D000409:Alanine / Q000031:analogs & derivatives* / Q000494:pharmacology; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology; D002460:Cell Line; D004847:Epithelial Cells / Q000166:cytology; D006801:Humans; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008168:Lung / Q000166:cytology / Q000378:metabolism*; D015363:Quinolones / Q000494:pharmacology*; D051197:Toll-Like Receptor 4 / Q000235:genetics / Q000378:metabolism*; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000378:metabolism*
D000894:Anti-Inflammatory Agents, Non-Steroidal; D008070:Lipopolysaccharides; D015363:Quinolones; C495345:TLR4 protein, human; D051197:Toll-Like Receptor 4; D014409:Tumor Necrosis Factor-alpha; C052785:rebamipide; D000409:Alanine