[Determination of beta-estradiol, bisphenol A, diethylstilbestrol and salbutamol in human urine by GC/MS].
To establish a GC/MS method for analysis of beta-estradiol (beta-E2), bisphenol A (BPA), diethylstilbestrol (DES) and salbutamol (SAL) in human urine.



Human urine samples were extracted by cleanert PCX and cleanert PEP cartridges; and derivatized after dried completely. beta-E2, BPA, DES and SAL in the extracts were measured by GC/MS method with DB-5MS capillary column and EI ion-source.



The calibration curves for beta-E2 in samples were linear over the concentration ranges of 1 approximately 300 ng ml(-1), for BPA were 1 approximately 200 ng ml(-1), for DES were 2 approximately 300 ng ml(-1) and for SAL were 0.01 approximately 1.2 microg ml(-1). The limits of detection were 0.15 ng ml(-1), 0.19 ng ml(-1), 0.23 ng ml(-1) and 1.0 ng ml(-1)for beta-E2, BPA, DES and SAL, respectively. The assay recoveries for beta-E2, BPA, DES and SAL ranged from 93.4 % approximately 110.5 %, 84.7 % approximately 104.9 %, 87.0 % approximately 105.4 % and 81.8 % approximately 96.8 %, respectively.



The established GC/MS method can detect beta-E2, BPA, DES and SAL in urine samples simultaneously, which can be used in routine assessment and monitoring of beta-E2, BPA, DES and SAL in human body.
Wu
Yuan-Yang
YY
Institute of Pharmacology, Toxicology and Biological Pharmaceutics, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058, China.
Shi
Wei-Xing
WX
Chen
Shu-Qing
SQ
D000420:Albuterol / Q000652:urine*; D001559:Benzhydryl Compounds; D004054:Diethylstilbestrol / Q000652:urine*; D004785:Environmental Pollutants / Q000652:urine; D004958:Estradiol / Q000652:urine*; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D010636:Phenols / Q000652:urine*; D000098826:Bisphenol A Compounds
D000420:Albuterol; D001559:Benzhydryl Compounds; D004054:Diethylstilbestrol; D004785:Environmental Pollutants; D004958:Estradiol; D010636:Phenols; C006780:bisphenol A; D000098826:Bisphenol A Compounds