title: Development of an analytical methodology for simultaneous determination of vincristine and doxorubicin in pharmaceutical preparations for oncology by HPLC-UV.
abstract: A high-performance liquid chromatography-UV methodology (lambda=230 nm) was developed and validated for the simultaneous determination of vincristine and doxorubicin in pharmaceutical preparations used in oncology. The chromatography was carried out on a C18 column using acetonitrile 90% in water-potassium hydrogenphosphate buffer 50 mM, pH 3.2+/-0.1 (32:68, v/v) as mobile phase at a flow rate of 1.5 mL/min. The method proved to be specific, exact, and accurate, in accordance with the ICH standards, presenting linearity in the 1-5 microg/mL and 5-100 microg/mL intervals, and detection (0.19x0.51 microg/mL) and quantification (0.63x1.7 microg/mL) limits for vincristine and doxorubicin, respectively.
authors.affiliation: Grupo de Bromatologia-Centro de Estudos Farmacêuticos, Faculdade de Farmácia da Universidade de Coimbra, 3000-295 Coimbra, Portugal.
authors.forename: Ana Sofia
authors.initials: AS
authors.lastname: Rodrigues
authors.forename: Ana Rita
authors.initials: AR
authors.lastname: Lopes
authors.forename: Ana
authors.initials: A
authors.lastname: Leão
authors.forename: Alda
authors.initials: A
authors.lastname: Couceiro
authors.forename: Ana Bela Sarmento
authors.initials: AB
authors.lastname: Ribeiro
authors.forename: Fernando
authors.initials: F
authors.lastname: Ramos
authors.forename: Maria Irene
authors.initials: MI
authors.lastname: Noronha da Silveira
authors.forename: Catarina
authors.initials: C
authors.lastname: Resende de Oliveira
mesh_terms: D000970:Antineoplastic Agents / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004317:Doxorubicin / Q000032:analysis*; D006863:Hydrogen-Ion Concentration; D004364:Pharmaceutical Preparations / Q000737:chemistry*; D012015:Reference Standards; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet / Q000379:methods*; D014750:Vincristine / Q000032:analysis*
chemical_list: D000970:Antineoplastic Agents; D004364:Pharmaceutical Preparations; D014750:Vincristine; D004317:Doxorubicin