Glioma stem cells: better flat than round.
Glioma-initiating cells/glioma stem cells (GIC/GSCs) are grown in vitro using a cumbersome and inefficient spheroid assay. In this issue of Cell Stem Cell, Pollard and coworkers present a protocol for the efficient derivation of GIC/GSC lines that may greatly improve the isolation and the potential clinical application of these cells.
Woolard
Kevin
K
Neuro-Oncology Branch, National Cancer Institute, National Institutes of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD 20892, USA.
Fine
Howard A
HA
D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods*; D048430:Cell Shape; D005910:Glioma / Q000473:pathology*; D006801:Humans; D014411:Neoplastic Stem Cells / Q000166:cytology*