[Hepatitis B virus P22(e) inhibit hepatocyte apoptosis via nuclear factor kappa B].
OBJECTIVE
To test whether nuclear factor kappa B plays an important role in the apoptosis-inhibitory effect of hepatitis B virus (HBV) P22(e) protein.


METHODS
HepG2 cells were transfected with recombination plasmid pEGFP-HBVP22(e). The Act-D and TNF alpha were used to induce apoptosis. NF-kappa B inhibitor ALLN were used to inhibit the signaling pathway. The activation of NF-kappa B was EMSA, and the nulear translocation of NF-kappa B was determined by immuno-staining.


RESULTS
Laser scanning confocal microscopy and EMSA indicated that HBV P22(e) protein enhanced the nuclear translocation of NF-kappa B after apoptosis induction. ALLN treatment inhibited the nuclear translocation of NF-kappa B, and blocked the apoptosis-inhibiting effect of HBV P22(e) protein.


CONCLUSIONS
This study indicates that HBV P22(e) protein inhibits apoptosis of hepatocyte via the NF-kappa B signaling pathway.
DIAO
Zhi-hong
ZH
Department of Infectious Diseases, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
ZHANG
Ming-xia
MX
ZHU
You-fu
YF
SHI
Yu-ling
YL
HOU
Jin-lin
JL
D017209:Apoptosis; D006528:Carcinoma, Hepatocellular / Q000378:metabolism*; D056945:Hep G2 Cells; D006512:Hepatitis B Core Antigens / Q000378:metabolism*; D006515:Hepatitis B virus / Q000235:genetics; D006801:Humans; D007976:Leupeptins / Q000494:pharmacology; D008113:Liver Neoplasms / Q000378:metabolism*; D016328:NF-kappa B / Q000037:antagonists & inhibitors* / Q000378:metabolism*; D010957:Plasmids; D015398:Signal Transduction / Q000187:drug effects; D014162:Transfection; D014758:Viral Core Proteins / Q000378:metabolism*
D006512:Hepatitis B Core Antigens; D007976:Leupeptins; D016328:NF-kappa B; D014758:Viral Core Proteins; C534392:p22 core protein, hepatitis B virus