title: Improved method for Duddingtonia flagrans chlamydospores production for livestock use.
abstract: Duddingtonia flagrans is a nematode-trapping fungus responsible for attacking larval stages of helminthes in pasture. D. flagrans chlamydospores were produced using a two-step liquid/solid culture system. The inoculum grown in liquid medium was transferred to a rice substrate and kept at room temperature for 30 days. Grains were washed, filtered and centrifuged. The pellet was lyophilized and the obtained yield averaged 1 x 10(5) chlamydospores per gram of dried material. The lyophilized chlamydospores showed a trapping rate of 69% of infective larvae in vitro and were excreted entirely in ovine faeces. The results showed that most of the chlamydospores remained intact and viable after the lyophilization process.
authors.affiliation: Laboratório de Pesquisas Micológicas, Departamento de Microbiologia e Parasitologia, Universidade Federal de Santa Maria, 97105-900, Santa Maria, RS, Brazil. janio.santurio@gmail.com
authors.forename: Janio M
authors.initials: JM
authors.lastname: Santurio
authors.forename: Régis A
authors.initials: RA
authors.lastname: Zanette
authors.forename: Aleksandro S
authors.initials: AS
authors.lastname: Da Silva
authors.forename: Mario L
authors.initials: ML
authors.lastname: De La Rue
authors.forename: Silvia G
authors.initials: SG
authors.lastname: Monteiro
authors.forename: Sydney H
authors.initials: SH
authors.lastname: Alves
mesh_terms: D000818:Animals; D001203:Ascomycota / Q000502:physiology*; D005612:Freeze Drying / Q000662:veterinary*; D007814:Larva / Q000382:microbiology; D009348:Nematoda / Q000382:microbiology*; D010572:Pest Control, Biological / Q000379:methods*; D013172:Spores, Fungal / Q000502:physiology*