A 1 : 2 copper(II)-tripeptide complex for DNA binding and cleavage agent under physiological conditions.
A 1 : 2 copper-tripeptide complex, [Cu(II)(Boc-His-Gly-His-OMe)(2)](2+), was synthesized and structurally characterized. The absorption band at 577 nm suggests a square-planar geometry around Cu(II). The DNA-binding and DNA-cleavage properties of the Cu(II) complex were investigated. The complex binds to calf thymus DNA (CT DNA) in an intercalative fashion and cleaves plasmid pUC-19 DNA hydrolytically at micromolar concentrations under physiological conditions. The intrinsic binding constant (K(b)=1.2x10(2) M(-1)) for the binding of Cu-tripeptide complex with DNA suggests that this complex is suitable for rapid diffusion on the pharmacological time scale.
Reddy
Pulimamidi R
PR
Department of Chemistry, Osmania University, Hyderabad, India.
Manjula
Pallerla
P
Chakraborty
Tushar K
TK
Samanta
Rajarshi
R
D003300:Copper / Q000737:chemistry*; D004247:DNA / Q000737:chemistry*; D053837:DNA Cleavage / Q000187:drug effects*; D009842:Oligopeptides / Q000737:chemistry*; D009942:Organometallic Compounds / Q000737:chemistry*; D010455:Peptides / Q000737:chemistry*
D009842:Oligopeptides; D009942:Organometallic Compounds; D010455:Peptides; C542668:bis(tert-butoxycarbonyl-histidyl-glycyl-histidine methyl ester)copper(II); D003300:Copper; D004247:DNA