Nitric oxide-releasing S-nitrosothiol-modified xerogels.
The synthesis, material characterization, and in vitro biocompatibility of S-nitrosothiol (RSNO)-modified xerogels are described. Thiol-functionalized xerogel films were formed by hydrolysis and co-condensation of 3-mercaptopropyltrimethoxysilane (MPTMS) and methyltrimethoxysilane (MTMOS) sol-gel precursors at varying concentrations. Subsequent thiol nitrosation via acidified nitrite produced RSNO-modified xerogels capable of generating nitric oxide (NO) for up to 2 weeks under physiological conditions. Xerogels also exhibited NO generation upon irradiation with broad-spectrum light or exposure to copper, with NO fluxes proportional to wattage and concentration, respectively. Xerogels were capable of storing up to approximately 1.31 micromol NO mg(-1), and displayed negligible fragmentation over a 2-week period. Platelet and bacterial adhesion to nitrosated films was reduced compared to non-nitrosated controls, confirming the antithrombotic and antibacterial properties of the NO-releasing materials. Fibroblast cell viability was maintained on the xerogel surfaces illustrating the promise of RSNO-modified xerogels as biomedical device coatings.
Riccio
Daniel A
DA
Department of Chemistry, Caudill Laboratories, University of North Carolina, CB#3290, Chapel Hill, NC 27599, USA.
Dobmeier
Kevin P
KP
Hetrick
Evan M
EM
Privett
Benjamin J
BJ
Paul
Heather S
HS
Schoenfisch
Mark H
MH
D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D000991:Antithrombins / Q000494:pharmacology; D001422:Bacterial Adhesion / Q000187:drug effects; D001792:Blood Platelets / Q000187:drug effects / Q000648:ultrastructure; D002470:Cell Survival / Q000187:drug effects; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects; D005782:Gels / Q000378:metabolism*; D051379:Mice; D008826:Microbial Sensitivity Tests; D009569:Nitric Oxide / Q000378:metabolism*; D017646:Organosilicon Compounds; D015539:Platelet Activation / Q000187:drug effects; D011550:Pseudomonas aeruginosa / Q000187:drug effects; D026403:S-Nitrosothiols / Q000378:metabolism*; D012821:Silanes / Q000378:metabolism; D034421:Sus scrofa
D000900:Anti-Bacterial Agents; D000991:Antithrombins; D005782:Gels; D017646:Organosilicon Compounds; D026403:S-Nitrosothiols; D012821:Silanes; C071718:methyltrimethoxysilane; D009569:Nitric Oxide; C102833:(3-mercaptopropyl)trimethoxysilane