title: Down-regulation of HLA class I antigen-processing machinery components in esophageal squamous cell carcinomas: association with disease progression.
abstract: OBJECTIVE
  Lack of human leukocyte antigen (HLA) presentation has been proposed to contribute to the immune evasion of cancer cells in some cancers including esophageal cancer. The aim of this study was to examine the expression of HLA class I antigen and the antigen-processing machinery (APM) components in esophageal squamous cell carcinoma (ESCC) lesions and to assess their association with histopathological characteristics.
  
  
  METHODS
  A total of 143 formalin-fixed, paraffin-embedded ESCC lesions collected in two hospitals in Shandong Province of China were studied. The expression levels were determined by immunohistochemistry.
  
  
  RESULTS
  TAP1, TAP2, LMP2, LMP7, beta2m, and HLA class I antigen were lost or down-regulated in 30.8%, 35.0%, 45.0%, 48.0%, 56.0%, and 60.8% of the ESCC lesions tested, respectively. The loss of or down-regulated expressions of HLA class I, beta2m, TAP1, LMP2, and LMP7 in tumor lesions were all significantly correlated to tumor grade and lymph node status. Expression of HLA class I antigens was strongly correlated to the expression levels of beta2m, TAP1, TAP2, LMP2, and LMP7, suggesting APM component defects as a mechanism underlying HLA class I antigen down-regulation in ESCC lesions. Expression of APM components and HLA class I antigens was significantly associated with the extent of intratumoral T-cell infiltration.
  
  
  CONCLUSIONS
  Our results indicate that lack or reduction of HLA class I antigens and expression of APM components in ESCC may render some tumor cells to escape the immunosurveillance mediated by CD8(+) T cells and contribute to the clinical course of ESCC.
authors.affiliation: Department of Cellular and Molecular Biology, Cancer Institute & Hospital, Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing, China. liuqiao80@gmail.com
authors.forename: Qiao
authors.initials: Q
authors.lastname: Liu
authors.forename: Chunyan
authors.initials: C
authors.lastname: Hao
authors.forename: Peng
authors.initials: P
authors.lastname: Su
authors.forename: Juanhong
authors.initials: J
authors.lastname: Shi
mesh_terms: D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D002294:Carcinoma, Squamous Cell / Q000276:immunology* / Q000473:pathology; D002681:China; D018450:Disease Progression; D015536:Down-Regulation; D004938:Esophageal Neoplasms / Q000276:immunology* / Q000473:pathology; D005260:Female; D005805:Genes, MHC Class I / Q000276:immunology*; D006801:Humans; D008297:Male; D008875:Middle Aged