Amlodipine alone or combined with terazosin improves lower urinary tract disorder in rat models of benign prostatic hyperplasia or detrusor instability: focus on detrusor overactivity.
OBJECTIVE
To investigate the effects of amlodipine, a dihydropyridine calcium-channel blocker, alone or combined with terazosin, on urodynamics in rats with benign prostatic hyperplasia (BPH) and in female rats with detrusor instability (DI).


METHODS
The rat model of BPH was induced by subcutaneous injection of testosterone (0.5 mg per rat for 21 days). The female rat model of DI was induced by partial bladder outlet ligation for 6 weeks. The rats were intragastrically administered with assigned drugs (amlodipine, terazosin or both combined) for 14 days in three experiments. Continuous cystometry was assessed in rats under urethane anaesthesia.


RESULTS
Amlodipine at 0.5, 1 and 3 mg/kg significantly decreased the bladder index (BI), threshold pressure (TP), and micturition pressure (MP), and significantly increased intermicturition duration (IMD) in BPH rats. Moreover, amlodipine 0.5 mg/kg plus terazosin 0.4 mg/kg gave the greatest improvements in all urodynamic variables, with a greater decrease in BI, TP, MP and greater increase in IMD than with either of the drugs used alone in BPH and DI rats, and had a similar efficacy to terazosin 1 mg/kg. The combined treatment also gave a greater decrease in nonvoiding bladder contractions in DI rats.


CONCLUSIONS
Amlodipine alone or combined with terazosin might have the potential to alleviate lower urinary tract symptoms (LUTS). The combined therapy appears to be more suitable for LUTS with predominantly irritative symptoms.
Liu
Hai-Peng
HP
School of Life Science, University of Science and Technology of China, Hefei, Anhui, China.
Chen
Guang-Liang
GL
Liu
Ping
P
Xu
Xi-Ping
XP
D000317:Adrenergic alpha-Antagonists / Q000627:therapeutic use*; D017311:Amlodipine / Q000627:therapeutic use*; D000818:Animals; D002121:Calcium Channel Blockers / Q000627:therapeutic use*; D004359:Drug Therapy, Combination; D005260:Female; D008297:Male; D011224:Prazosin / Q000031:analogs & derivatives* / Q000627:therapeutic use; D011470:Prostatic Hyperplasia / Q000150:complications / Q000188:drug therapy*; D053448:Prostatism / Q000188:drug therapy / Q000209:etiology; D051381:Rats; D017207:Rats, Sprague-Dawley; D016896:Treatment Outcome; D053201:Urinary Bladder, Overactive / Q000188:drug therapy*
D000317:Adrenergic alpha-Antagonists; D002121:Calcium Channel Blockers; D017311:Amlodipine; C041226:Terazosin; D011224:Prazosin