Preparative isolation and purification of anthocyanins from purple sweet potato by high-speed counter-current chromatography.
High-speed counter-current chromatography (HSCCC) was applied to the preparative isolation and purification of peonidin 3-O-(6-O-(E)-caffeoyl-2-O-beta-D-glucopyranosyl-beta-D-glucopyranoside)-5-O-beta-D-glucoside (1), cyanidin 3-O-(6-O-p-coumaroyl)-beta-D-glucopyranoside (2), peonidin 3-O-(2-O-(6-O-(E)-caffeoyl-beta-D-glucopyranosyl)-6-O-(E)-caffeoyl-beta-D-glucopyranoside)-5-O-beta-D-glucopyranoside (3), peonidin 3-O-(2-O-(6-O-(E)-feruloyl-beta-D-glucopyranosyl)-6-O-(E)-caffeoyl-beta-D-glucopyranoside)-5-O-beta-D-glucopyranoside (4) from purple sweet potato. Separation of crude extracts (200 mg) from the roots of purple sweet potato using methyl tert-butyl ether/n-butanol/acetonitrile/water/trifluoroacetic acid (1:4:1:5:0.01, v/v) as the two-phase solvent system yielded 1 (15 mg), 2 (7 mg), 3 (10 mg), and 4 (12 mg). The purities of 1-4 were 95.5%, 95.0%, 97.8%, and 96.3%, respectively, as determined by HPLC. Compound 2 was isolated from purple sweet potato for the first time. The chemical structures of these components were identified by 1H NMR, 13C NMR and ESI-MS(n).
Qiu
Fan
F
Department of Natural Medicinal Chemistry, China Pharmaceutical University, Nanjing, People's Republic of China.
Luo
Jianguang
J
Yao
Shun
S
Ma
Li
L
Kong
Lingyi
L
D000872:Anthocyanins / Q000302:isolation & purification* / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods; D003377:Countercurrent Distribution / Q000295:instrumentation / Q000379:methods*; D065089:Glycoside Hydrolase Inhibitors; D027723:Ipomoea batatas / Q000737:chemistry*; D015394:Molecular Structure; D010936:Plant Extracts / Q000737:chemistry; D012997:Solvents / Q000737:chemistry; D000520:alpha-Glucosidases / Q000378:metabolism
D000872:Anthocyanins; D065089:Glycoside Hydrolase Inhibitors; D010936:Plant Extracts; D012997:Solvents; D000520:alpha-Glucosidases