title: Comparability of ELISA and toxin neutralization to measure immunogenicity of Protective Antigen in mice, as part of a potency test for anthrax vaccines.
abstract: Complexities of lethal challenge models have prompted the investigation of immunogenicity assays as potency tests of anthrax vaccines. An ELISA and a lethal toxin neutralization assay (TNA) were used to measure antibody response to Protective Antigen (PA) in mice immunized once with either a commercial or a recombinant PA (rPA) vaccine formulated in-house. Even though ELISA and TNA results showed correlation, ELISA results may not be able to accurately predict TNA results in this single immunization model.
authors.affiliation: Oswaldo Cruz Foundation, Research Center Rene Rachou, Av. Augusto de Lima 1715, Belo Horizonte, MG 30190-002, Brazil.
authors.forename: P M
authors.initials: PM
authors.lastname: Parreiras
authors.forename: L A
authors.initials: LA
authors.lastname: Sirota
authors.forename: L D
authors.initials: LD
authors.lastname: Wagner
authors.forename: S L
authors.initials: SL
authors.lastname: Menzies
authors.forename: J L
authors.initials: JL
authors.lastname: Arciniega
mesh_terms: D000818:Animals; D000881:Anthrax / Q000276:immunology / Q000517:prevention & control*; D022122:Anthrax Vaccines / Q000276:immunology*; D000907:Antibodies, Bacterial / Q000276:immunology; D000942:Antigens, Bacterial / Q000276:immunology*; D001427:Bacterial Toxins / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D051379:Mice; D009500:Neutralization Tests; D011994:Recombinant Proteins / Q000276:immunology; D014614:Vaccines, Synthetic / Q000276:immunology
chemical_list: D022122:Anthrax Vaccines; D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001427:Bacterial Toxins; D011994:Recombinant Proteins; D014614:Vaccines, Synthetic; C030325:anthrax toxin