[Study on enzyme-solvent extraction process of flavonoids from leaves of Ginkgo biloba].
OBJECTIVE
To study the optimum enzyme-solvent extraction conditions of flavonoids from leaves of Ginkgo biloba.


METHODS
To optimize the process by single factor experiments and orthogonal test according to the yield of flavonoids.


RESULTS
The optimum extraction conditions were established as follows: pH 5.7, the concentration of enzyme 0.4 mg/mL, enzymatic hydrolysis temperature 45 degrees C, enzymatic hydrolysis time 2 h, the rate of liquid to material 20:1, extraction temperature 70 degrees C, extraction time 2 h, the concentration of ethanol 70%, the average yield of flavonoids from leaves of Ginkgo biloba was 3.62%.


CONCLUSIONS
The method is simple and can increase material utility effectively.
Ouyang
Na-na
NN
Materials Science and Engineering Institute, Central South University of Forestry and Technology, Changsha 410004, China. oynn@netease.com
Li
Xiang-zhou
XZ
Luo
Zheng
Z
D000704:Analysis of Variance; D000431:Ethanol; D005419:Flavonoids / Q000032:analysis / Q000302:isolation & purification*; D020441:Ginkgo biloba / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D018515:Plant Leaves / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D012997:Solvents; D013678:Technology, Pharmaceutical / Q000379:methods*; D013696:Temperature; D013997:Time Factors
D005419:Flavonoids; D012997:Solvents; D000431:Ethanol