title: Elicitation of silymarin in cell cultures of Silybum marianum: effect of subculture and repeated addition of methyl jasmonate.
abstract: Production of silymarin and the effect of the elicitor, methyl jasmonate (MeJA), was monitored in cell cultures of Silybum marianum over 4 years. Silymarin concentrations gradually declined after prolonged subculture, making the success of elicitor strategy limited in long-term cultures. The continuous presence of MeJA in cultures for an extended period was necessary for induction of silymarin accumulation. A repeated elicitor strategy was not a good option for improving silymarin productivity in batch cultures. Removal of medium from elicited cultures and addition of fresh medium avoided the toxic effects of elicitor accumulation, allowing the system to respond to a repeated MeJA treatment without loss of productivity.
authors.affiliation: Department of Plant Physiology, Faculty of Pharmacy, University of Salamanca, Campus Miguel de Unamuno, 37007, Salamanca, Spain.
authors.forename: Maria Angeles
authors.initials: MA
authors.lastname: Sánchez-Sampedro
authors.forename: Jorge
authors.initials: J
authors.lastname: Fernández-Tárrago
authors.forename: Purificación
authors.initials: P
authors.lastname: Corchete
mesh_terms: D000085:Acetates / Q000378:metabolism*; D018929:Cell Culture Techniques; D003517:Cyclopentanes / Q000378:metabolism*; D020944:Silybum marianum / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism*; D054883:Oxylipins / Q000378:metabolism*; D012838:Silymarin / Q000096:biosynthesis*
chemical_list: D000085:Acetates; D003517:Cyclopentanes; D054883:Oxylipins; D012838:Silymarin; C072239:methyl jasmonate