title: Saccharospirillum salsuginis sp. nov., a gammaproteobacterium from a subterranean brine.
abstract: A novel Gram-negative, non-sporulating, moderately halophilic, facultatively alkaliphilic, catalase- and oxidase-positive, obligately aerobic bacterium, strain YIM-Y25(T), was isolated from a subterranean brine sample collected from a salt mine in Yunnan, south-west China. Cells were spirilla, motile by monopolar flagella, with meso-diaminopimelic acid in the cell-wall peptidoglycan. Growth occurred with 1-15 % (w/v) NaCl (optimum 5 %), at pH 6.0-10.0 (optimum pH 8.0) and at 15-50 degrees C (optimum 35-40 degrees C). Ubiquinone Q-8 was the predominant respiratory quinone. Polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylmonomethylamine and an unidentified phospholipid. The major cellular fatty acids were C(18 : 1)omega7c, iso-C(16 : 0), C(16 : 0) and C(16 : 1). The genomic DNA G+C content was 58.5 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain YIM-Y25(T) was most closely related to the type strain of the sole recognized species of the genus Saccharospirillum, Saccharospirillum impatiens EL-105(T) (sequence similarity 97.0 %), and these two strains formed a robust lineage in the phylogenetic tree. The level of DNA-DNA relatedness between them was 12.6 %. The combination of phylogenetic analysis, phenotypic differences, chemotaxonomic properties and DNA-DNA hybridization data supported the view that this strain represents a novel species of the genus Saccharospirillum, for which the name Saccharospirillum salsuginis sp. nov. is proposed, with YIM-Y25(T) (=CCTCC AA 207033(T) =KCTC 22169(T)) as the type strain.
authors.affiliation: Yunnan Institute of Microbiology, Yunnan University, Kunming, Yunnan 650091, PR China.
authors.forename: Yi-Guang
authors.initials: YG
authors.lastname: Chen
authors.forename: Xiao-Long
authors.initials: XL
authors.lastname: Cui
authors.forename: Qin-Yuan
authors.initials: QY
authors.lastname: Li
authors.forename: Yong-Xia
authors.initials: YX
authors.lastname: Wang
authors.forename: Shu-Kun
authors.initials: SK
authors.lastname: Tang
authors.forename: Zhu-Xiang
authors.initials: ZX
authors.lastname: Liu
authors.forename: Meng-Liang
authors.initials: ML
authors.lastname: Wen
authors.forename: Qian
authors.initials: Q
authors.lastname: Peng
authors.forename: Li-Hua
authors.initials: LH
authors.lastname: Xu
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D002681:China; D004269:DNA, Bacterial / Q000032:analysis; D004275:DNA, Ribosomal / Q000032:analysis; D005227:Fatty Acids / Q000032:analysis; D020563:Gammaproteobacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010641:Phenotype; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012965:Sodium Chloride; D012988:Soil Microbiology; D013045:Species Specificity
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D012965:Sodium Chloride