title: Recognition of anti-mycolic acid antibody at self-assembled mycolic acid antigens on a gold electrode: a potential impedimetric immunosensing platform for active tuberculosis.
abstract: Electrochemical impedimetric recognition by anti-mycolic acid antibodies, present in tuberculosis (TB)-positive human serum co-infected with human immunodeficiency virus (HIV), of mycolic acids (MA) integrated into a self-assembled monolayer of N-(2-mercaptoethyl)octadecanamide on a gold electrode is described, proving that the MA-based electrode can satisfactorily discriminate between a TB-positive and a TB-negative serum, thus offering promise as a potential impedimetric immunosensing platform for active tuberculosis.
authors.affiliation: Department of Chemistry, University of Pretoria, South Africa.
authors.forename: Nsovo S
authors.initials: NS
authors.lastname: Mathebula
authors.forename: Jeseelan
authors.initials: J
authors.lastname: Pillay
authors.forename: Gianna
authors.initials: G
authors.lastname: Toschi
authors.forename: Jan A
authors.initials: JA
authors.lastname: Verschoor
authors.forename: Kenneth I
authors.initials: KI
authors.lastname: Ozoemena
mesh_terms: D000577:Amides / Q000737:chemistry; D000906:Antibodies / Q000032:analysis* / Q000276:immunology*; D000941:Antigens / Q000276:immunology; D015415:Biomarkers / Q000032:analysis; D017097:Electric Impedance; D004563:Electrochemistry; D004566:Electrodes; D006046:Gold / Q000737:chemistry*; D015658:HIV Infections / Q000097:blood / Q000150:complications; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D009171:Mycolic Acids / Q000737:chemistry / Q000276:immunology*; D012680:Sensitivity and Specificity; D014376:Tuberculosis / Q000097:blood / Q000150:complications / Q000175:diagnosis* / Q000276:immunology*
chemical_list: D000577:Amides; D000906:Antibodies; D000941:Antigens; D015415:Biomarkers; D009171:Mycolic Acids; D006046:Gold