Cholesterol-modified superporous poly(2-hydroxyethyl methacrylate) scaffolds for tissue engineering.
Modifications of poly(2-hydroxyethyl methacrylate) (PHEMA) with cholesterol and laminin have been developed to design scaffolds that promote cell-surface interaction. Cholesterol-modified superporous PHEMA scaffolds have been prepared by the bulk radical copolymerization of 2-hydroxyethyl methacrylate (HEMA), cholesterol methacrylate (CHLMA) and the cross-linking agent ethylene dimethacrylate (EDMA) in the presence of ammonium oxalate crystals to introduce interconnected superpores in the matrix. With the aim of immobilizing laminin (LN), carboxyl groups were also introduced to the scaffold by the copolymerization of the above monomers with 2-[(methoxycarbonyl)methoxy]ethyl methacrylate (MCMEMA). Subsequently, the MCMEMA moiety in the resulting hydrogel was hydrolyzed to [2-(methacryloyloxy)ethoxy]acetic acid (MOEAA), and laminin was immobilized via carbodiimide and N-hydroxysulfosuccinimide chemistry. The attachment, viability and morphology of mesenchymal stem cells (MSCs) were evaluated on both nonporous and superporous laminin-modified as well as laminin-unmodified PHEMA and poly(2-hydroxyethyl methacrylate-co-cholesterol methacrylate) P(HEMA-CHLMA) hydrogels. Neat PHEMA and laminin-modified PHEMA (LN-PHEMA) scaffolds facilitated MSC attachment, but did not support cell spreading and proliferation; the viability of the attached cells decreased with time of cultivation. In contrast, MSCs spread and proliferated on P(HEMA-CHLMA) and LN-P(HEMA-CHLMA) hydrogels.
Kubinová
Sárka
S
Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, Vídenská 1083, 142 20 Prague 4, Czech Republic.
Horák
Daniel
D
Syková
Eva
E
D000818:Animals; D002234:Carbodiimides / Q000737:chemistry; D002452:Cell Count; D002470:Cell Survival / Q000187:drug effects; D002784:Cholesterol / Q000378:metabolism*; D020100:Hydrogels / Q000737:chemistry; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000187:drug effects; D008689:Methacrylates / Q000737:chemistry / Q000494:pharmacology*; D018613:Microscopy, Confocal; D008855:Microscopy, Electron, Scanning; D016062:Porosity / Q000187:drug effects; D051381:Rats; D012996:Solutions; D013056:Spectrophotometry, Ultraviolet; D023822:Tissue Engineering; D054457:Tissue Scaffolds / Q000737:chemistry*
D002234:Carbodiimides; D020100:Hydrogels; D008689:Methacrylates; D012996:Solutions; C005044:hydroxyethyl methacrylate; D002784:Cholesterol