Evaluation of use of a dicationic liquid stationary phase in the fast and conventional gas chromatographic analysis of health-hazardous C18 cis/trans fatty acids.
The present research is focused on the evaluation of one 0.10 mm i.d. and two 0.25 mm i.d., ionic liquid (IL) stationary phase [1,9-di(3-vinyl-imidazolium) nonane bis(trifluoromethyl) sulfonyl imidate] columns, with lengths of 12 (the microbore capillary), 30 and 100 m, in the GC analysis of cis/trans fatty acid methyl esters (FAMEs). The selectivity of the IL columns toward a series of standard C(18:1), C(18:2), and C(18:3) geometric isomers (a group of 22 compounds was subjected to GC analysis) was compared to the performance of a widely used column in the cis/trans FAMEs analysis field, viz., a 100 m x 0.25 mm i.d. capillary with a 0.20 microm stationary phase film of bis-cyanopropyl polysiloxane (SP-2560). The selectivity provided by the IL phase was superior if compared to that of the other well-established capillary. An optimized IL method, using the longer column, was subjected to validation: retention time and peak area intraday precision (n = 5) were good, with RSD values lower than 0.07% and 6.6%, respectively; LODs (considering a S/N of 3) for C(18:1Delta)(9tr) and C(18:2Delta)(9tr,12tr) were 0.15 (7.3 ppm) and 0.18 ng (9.1 ppm) on-column, respectively, while LOQs (considering a S/N of 10) were 0.49 (24.3 ppm) and 0.60 ng (30.2 ppm), respectively; the method was found to be linear, for both trans FAMEs, in the 10-2000 ppm range. For the evaluation of accuracy, a hydrogenated margarine, spiked with known amounts of C(18:3Delta)(9c,12c,15c), was subjected to analysis using C(13:0) as an internal standard.
Ragonese
Carla
C
Dipartimento Farmaco-Chimico, Facoltà di Farmacia, Università dei Messina, Viale Annunziata, I-98168, Messina, Italy.
Tranchida
Peter Quinto
PQ
Dugo
Paola
P
Dugo
Giovanni
G
Sidisky
Leonard M
LM
Robillard
Mark V
MV
Mondello
Luigi
L
D002849:Chromatography, Gas / Q000379:methods*; D005069:Evaluation Studies as Topic; D052578:Ionic Liquids / Q000737:chemistry*; D007536:Isomerism; D044242:Trans Fatty Acids / Q000032:analysis*
D052578:Ionic Liquids; D044242:Trans Fatty Acids