Toll-like receptor-mediated anti-inflammatory action of glaucine and oxoglaucine.
Two isochinoline alkaloids, glaucine and oxoglaucine were investigated for their suggested anti-inflammatory influence concerning nitric oxide and cytokine production. Mouse peritoneal macrophages were stimulated with different Toll-like receptor (TLR) ligands such as LPS for TLR4, zymosan for TLR2 and CpG for TLR9. The alkaloids inhibited TNF-alpha and IL-6 production induced by these ligands. In regard to IL-12 suppressive effect was registered in the case of CpG stimulation. Glaucine succeeded to enhance LPS and zymosan-induced IL-10 production. The reduction of pro-inflammatory cytokines and increase of anti-inflammatory IL-10 are indicative for their use in different acute and chronic inflammatory diseases.
Remichkova
Mimi
M
Department of Immunology, The Stephan Angeloff Institute of Microbiology, 1113 Sofia, Bulgaria.
Dimitrova
Petya
P
Philipov
Stefan
S
Ivanovska
Nina
N
D000470:Alkaloids / Q000494:pharmacology*; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D001058:Apomorphine / Q000031:analogs & derivatives* / Q000494:pharmacology; D001060:Aporphines / Q000494:pharmacology*; D016207:Cytokines / Q000378:metabolism; D007109:Immunity / Q000187:drug effects; D008070:Lipopolysaccharides; D008264:Macrophages / Q000187:drug effects* / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D009569:Nitric Oxide / Q000096:biosynthesis; D028101:Papaveraceae / Q000737:chemistry*; D035261:Plant Components, Aerial; D010936:Plant Extracts / Q000494:pharmacology*; D013329:Structure-Activity Relationship; D051193:Toll-Like Receptors / Q000378:metabolism*; D015054:Zymosan
D000470:Alkaloids; D000893:Anti-Inflammatory Agents; D001060:Aporphines; D016207:Cytokines; D008070:Lipopolysaccharides; D010936:Plant Extracts; D051193:Toll-Like Receptors; D009569:Nitric Oxide; C111587:oxoglaucine; D015054:Zymosan; D001058:Apomorphine; C005004:glaucine