title: Simultaneous determination of ofloxacin and ornidazole in pharmaceutical preparations by capillary zone electrophoresis.
abstract: A simple, rapid and validated capillary electrophoretic method has been developed for the separation and determination of ofloxacin and ornidazole in pharmaceutical formulations with detection at 230 nm. Optimal conditions for the quantitative separations were investigated. Analysis times shorter than 4 min were obtained using a background electrolyte solution consisting of 25 mmol/L phosphoric acid adjusted with 1 M Tris buffer to pH 8.5, with hydrodynamic injection of 5 s and 20 kV separation voltage. The validation criteria for accuracy, precision, linearity and limits of detection and quantitation were examined and discussed. An excellent linearity was obtained in concentration range 25-250 microg/mL. The detection limits for ofloxacin and ornidazole were 1.03 +/- 0.11 and 1.80 +/- 0.06 microg/mL, respectively. The proposed method has been applied for the analysis of ofloxacin and ornidazole both individually and in a combined dosage tablet formulation. The proposed validated method showed recoveries between 96.16 and 105.23% of the nominal contents.
authors.affiliation: School of Chemical Sciences, Universiti Sains Malaysia, Penang, Malaysia.
authors.forename: Khoo Lay
authors.initials: KL
authors.lastname: See
authors.forename: Abdalla A
authors.initials: AA
authors.lastname: Elbashir
authors.forename: Bahruddin
authors.initials: B
authors.lastname: Saad
authors.forename: Abdussalam Salhin Mohamed
authors.initials: AS
authors.lastname: Ali
authors.forename: Hassan Y
authors.initials: HY
authors.lastname: Aboul-Enein
mesh_terms: D000900:Anti-Bacterial Agents / Q000032:analysis*; D002021:Buffers; D019075:Electrophoresis, Capillary / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D015242:Ofloxacin / Q000032:analysis*; D009950:Ornidazole / Q000032:analysis*; D004364:Pharmaceutical Preparations / Q000737:chemistry*; D012015:Reference Standards; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet
chemical_list: D000900:Anti-Bacterial Agents; D002021:Buffers; D004364:Pharmaceutical Preparations; D009950:Ornidazole; D015242:Ofloxacin