Elicitation studies in cell suspension cultures of Cannabis sativa L.
Cannabis sativa L. plants produce a diverse array of secondary metabolites. Cannabis cell cultures were treated with biotic and abiotic elicitors to evaluate their effect on secondary metabolism. Metabolic profiles analysed by (1)H NMR spectroscopy and principal component analysis (PCA) showed variations in some of the metabolite pools. However, no cannabinoids were found in either control or elicited cannabis cell cultures. Tetrahydrocannabinolic acid (THCA) synthase gene expression was monitored during a time course. Results suggest that other components in the signaling pathway can be controlling the cannabinoid pathway.
Flores-Sanchez
Isvett Josefina
IJ
Pharmacognosy Department/Metabolomics, Institute of Biology, Gorlaeus Laboratories, P.O. Box 9502, Leiden University, 2300 RA Leiden, The Netherlands.
Pec
Jaroslav
J
Fei
Junni
J
Choi
Young Hae
YH
Dusek
Jaroslav
J
Verpoorte
Robert
R
D000704:Analysis of Variance; D002188:Cannabis / Q000187:drug effects / Q000201:enzymology* / Q000378:metabolism; D018929:Cell Culture Techniques; D002478:Cells, Cultured; D003517:Cyclopentanes / Q000494:pharmacology; D013759:Dronabinol / Q000031:analogs & derivatives* / Q000378:metabolism; D019746:Intramolecular Oxidoreductases / Q000235:genetics / Q000378:metabolism; D055442:Metabolome / Q000187:drug effects; D055432:Metabolomics / Q000379:methods; D000432:Methanol / Q000737:chemistry; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods; D054883:Oxylipins / Q000494:pharmacology; D010368:Pectins / Q000494:pharmacology; D010940:Plant Proteins / Q000378:metabolism*; D025341:Principal Component Analysis / Q000379:methods; D014867:Water / Q000737:chemistry
D003517:Cyclopentanes; D054883:Oxylipins; D010940:Plant Proteins; D014867:Water; C016780:11-nor-delta(9)-tetrahydrocannabinol-9-carboxylic acid; C011006:jasmonic acid; D013759:Dronabinol; D010368:Pectins; D019746:Intramolecular Oxidoreductases; C100677:delta(1)-tetrahydrocannabinolic acid synthase; D000432:Methanol