A tubular biocontainer: metal ion-induced 1D assembly of a molecularly engineered chaperonin.
GroEL(SP/MC), prepared by genetic and chemical modifications of group I chaperonin protein GroEL, site-specifically possesses approximately 28 photochromic (spiropyran [SP] and merocyanine [MC]) units in the entrance parts of its cavity. Addition of divalent metal ions such as Mg(2+) to a tris-HCl buffer solution of GroEL(SP/MC) results in one-dimensional (1D) assembly of GroEL(SP/MC), affording cylindrical hollow fibers with a very large aspect ratio; the longest fiber was approximately 2.5 microm long, corresponding to a 170-mer of GroEL(SP/MC) (MW approximately 1.4 x 10(8)). When such long fibers are mixed with EDTA, they are cut into short-chain oligomers and eventually into monomeric GroEL(SP/MC). Similar to GroEL, GroEL(SP/MC) possesses a large binding affinity toward denatured proteins. When GroEL(SP/MC) undergoes 1D assembly after incubation with a denatured protein, guest-containing cylindrical fibers result.
Biswas
Shuvendu
S
Department of Chemistry and Biotechnology, School of Engineering and Center for NanoBio Integration, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan.
Kinbara
Kazushi
K
Oya
Nobuhiro
N
Ishii
Noriyuki
N
Taguchi
Hideki
H
Aida
Takuzo
T
D001578:Benzopyrans / Q000737:chemistry; D002413:Cations, Divalent / Q000737:chemistry; D018834:Chaperonin 60 / Q000737:chemistry* / Q000235:genetics; D007211:Indoles / Q000737:chemistry; D008274:Magnesium / Q000737:chemistry*; D009574:Nitro Compounds / Q000737:chemistry; D011489:Protein Denaturation; D015202:Protein Engineering / Q000379:methods*; D011744:Pyrimidinones / Q000737:chemistry
D001578:Benzopyrans; D002413:Cations, Divalent; D018834:Chaperonin 60; D007211:Indoles; D009574:Nitro Compounds; D011744:Pyrimidinones; C088184:spiropyran; C003954:merocyanine dye; D008274:Magnesium