title: Evaluation of angiogenesis in colorectal carcinoma with multidetector-row CT multislice perfusion imaging.
abstract: To evaluate the correlation between 64 multidetector-row CT (64MDCT) perfusion imaging in colorectal carcinoma and microvessel density (MVD) and vascular endothelial growth factor (VEGF), 64MDCT perfusion imaging was performed in 33 patients with pathologically verified colorectal carcinoma. These images were analyzed with perfusion functional software, and time-density curves (TDC) were created for the region of interest (ROI) encompassing the tumor, the target artery and vein. The individual perfusion maps generated indicated blood flow (BF), blood volume (BV), mean transit time (MTT), and permeability-surface area product (PS). MVD and VEGF were evaluated by immunohistochemical staining with anti-CD34 and anti-VEGF, respectively. Correlations between MVD or VEGF with CT perfusion parameters and clinicopathological factors (Dukes' stages, invasion depth, and lymph node and liver metastasis) were also investigated. MVD in the colorectal carcinoma was 22.61+/-9.01 per x200 field. The scores obtained for VEGF expression were 4.15+/-1.09. VEGF staining was positive in 25 of 29 tumors (86.2%). There was no significant correlation between the presence of MVD, VEGF expression and clinicopathological factors (P>0.05). There was also no correlation between MVD, VEGF expression, and any dynamic CT parameters (P>0.05). The BV and MTT were significantly higher in tumors demonstrating serous coat invasion than in those without it (t=-2.63, -2.24, P=0.0137, 0.0331, respectively). BV was also significantly correlated with tumor size (r=0.41, P=0.02). Neither BF nor PS was correlated with clinicopathological factors. In conclusion, 64MDCT perfusion imaging, MVD, and VEGF may reflect angiogenic activity, but no significant correlation among these factors.
authors.affiliation: Department of Radiology, The First Affiliated Hospital of SunYat-sen University, Guangzhou 510080, China. fst1977@163.com
authors.forename: Shi-Ting
authors.initials: ST
authors.lastname: Feng
authors.forename: Can-Hui
authors.initials: CH
authors.lastname: Sun
authors.forename: Zi-Ping
authors.initials: ZP
authors.lastname: Li
authors.forename: Henry Ka-Fung
authors.initials: HK
authors.lastname: Mak
authors.forename: Zhen-Peng
authors.initials: ZP
authors.lastname: Peng
authors.forename: Huan-Yi
authors.initials: HY
authors.lastname: Guo
authors.forename: Quan-Fei
authors.initials: QF
authors.lastname: Meng
mesh_terms: D000230:Adenocarcinoma / Q000098:blood supply* / Q000000981:diagnostic imaging / Q000378:metabolism; D000328:Adult; D000368:Aged; D001783:Blood Flow Velocity; D001810:Blood Volume; D015179:Colorectal Neoplasms / Q000098:blood supply* / Q000000981:diagnostic imaging* / Q000378:metabolism; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D055806:Microvessels / Q000473:pathology; D008875:Middle Aged; D009389:Neovascularization, Pathologic / Q000000981:diagnostic imaging*; D055420:Perfusion Imaging; D014057:Tomography, X-Ray Computed; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism; D055815:Young Adult
chemical_list: D042461:Vascular Endothelial Growth Factor A