The fabrication and characterization of dicalcium phosphate dihydrate-modified magnetic nanoparticles and their performance in hyperthermia processes in vitro.
Many different types of magnetic particles have been developed for the purpose of hyperthermia cancer therapy. In this study, a magnetic nanoparticle based on dicalcium phosphate dihydrate (DCPD) was formed by co-precipitation method. Addition of different concentrations of ferrous chloride to DCPD can alter its material properties. Various physical, chemical and magnetic tests of the magnetic DCPD nanoparticles (mDCPD) were performed, including X-ray diffraction (XRD), inductively coupled plasma-optical emission spectrometer (ICP-OES), superconducting quantum interference device (SQUID), and transmission electron microscopy (TEM). The heating efficiency of mDCPD in alternating magnetic field was proved to be suitable for hyperthermia. The results of cytotoxicity tests (WST-1 and LDH assay) showed no harmful effect. The mDCPD showed relative cancer-killing ability without damaging normal cells in vitro.
Hou
Chun-han
CH
Institute of Biomedical Engineering, College of Medicine and College of Engineering, National Taiwan University, Taipei, Taiwan.
Chen
Ching-wei
CW
Hou
Sheng-mou
SM
Li
Yu-ting
YT
Lin
Feng-huei
FH
D000818:Animals; D001681:Biological Assay; D002130:Calcium Phosphates / Q000494:pharmacology*; D016923:Cell Death / Q000187:drug effects; D045744:Cell Line, Tumor; D006801:Humans; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008280:Magnetics; D051379:Mice; D008928:Mitochondria / Q000187:drug effects / Q000648:ultrastructure; D053758:Nanoparticles / Q000737:chemistry*; D009369:Neoplasms / Q000473:pathology; D010316:Particle Size; D013054:Spectrophotometry, Atomic; D013696:Temperature; D013778:Tetrazolium Salts; D013997:Time Factors; D014961:X-Ray Diffraction
C110656:2-(4-iodophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium; D002130:Calcium Phosphates; D013778:Tetrazolium Salts; D007770:L-Lactate Dehydrogenase; C494366:calcium phosphate, dibasic, dihydrate