title: The major outer membrane protein OprG of Pseudomonas aeruginosa contributes to cytotoxicity and forms an anaerobically regulated, cation-selective channel.
abstract: OprG of Pseudomonas aeruginosa is a member of the very large and widely distributed but poorly characterized OmpW (PF0392) family of outer membrane proteins. It was established here that OprG was highly transcribed in anaerobic environments rich in iron via the ANR regulator. In the absence of OprG, P. aeruginosa was significantly less cytotoxic toward human bronchial epithelial cells. Planar bilayer studies indicated that purified OprG formed cationic-selective channels with a conductance of 500 pS in 1 M KCl; however, contrary to previous reports, OprG did not appear to be involved in either iron or antibiotic uptake.
authors.affiliation: Centre for Microbial Diseases and Immunity Research, University of British Columbia, Lower Mall Research Station, Vancouver, BC, Canada.
authors.forename: Joseph B
authors.initials: JB
authors.lastname: McPhee
authors.forename: Sandeep
authors.initials: S
authors.lastname: Tamber
authors.forename: Manjeet
authors.initials: M
authors.lastname: Bains
authors.forename: Elke
authors.initials: E
authors.lastname: Maier
authors.forename: Shaan
authors.initials: S
authors.lastname: Gellatly
authors.forename: Andy
authors.initials: A
authors.lastname: Lo
authors.forename: Roland
authors.initials: R
authors.lastname: Benz
authors.forename: Robert E W
authors.initials: RE
authors.lastname: Hancock
mesh_terms: D000693:Anaerobiosis; D001425:Bacterial Outer Membrane Proteins / Q000378:metabolism* / Q000633:toxicity*; D002412:Cations / Q000378:metabolism*; D002460:Cell Line; D004847:Epithelial Cells / Q000382:microbiology; D020869:Gene Expression Profiling; D006801:Humans; D007473:Ion Channels / Q000378:metabolism* / Q000633:toxicity*; D007501:Iron / Q000378:metabolism; D011550:Pseudomonas aeruginosa / Q000378:metabolism* / Q000472:pathogenicity*; D020545:Respiratory Mucosa / Q000382:microbiology
chemical_list: D001425:Bacterial Outer Membrane Proteins; D002412:Cations; D007473:Ion Channels; C121501:OprG protein, Pseudomonas aeruginosa; D007501:Iron