title: Endogenous cGMP regulates adult longevity via the insulin signaling pathway in Caenorhabditis elegans.
abstract: G-proteins, including GPA-3, play an important role in regulating physiological responses in Caenorhabditis elegans. When confronted with an environmental stimulus such as dauer pheromone, or poor nutrients, C. elegans receives and integrates external signals through its nervous system (i.e. amphid neurons), which interprets and translates them into biological action. Here it is shown that a suppressed neuronal cGMP level caused by GPA-3 activation leads to a significant increase (47.3%) in the mean lifespan of adult C. elegans through forkhead transcription factor family O (FOXO)-mediated signal. A reduced neuronal cGMP level was found to be caused by an increased cGMP-specific phosphodiesterase activity at the transcriptional level. Our results using C. elegans mutants with specific deficits in TGF-beta and FOXO RNAi system suggest a mechanism in that cGMP, TGF-beta, and FOXO signaling interact to differentially produce the insulin-like molecules, ins-7 and daf-28, causing suppression of the insulin/IGF-1 pathway and promoting lifespan extension. Our findings provide not only a new mechanism of cGMP-mediated induction of longevity in adult C. elegans but also a possible therapeutic strategy for neuronal disease, which has been likened to brain diabetes.
authors.affiliation: Department of Biochemistry, Yonsei Proteome Research Center, Yonsei University, Seoul, Korea.
authors.forename: Jeong-Hoon
authors.initials: JH
authors.lastname: Hahm
authors.forename: Sunhee
authors.initials: S
authors.lastname: Kim
authors.forename: Young-Ki
authors.initials: YK
authors.lastname: Paik
mesh_terms: D000818:Animals; D017173:Caenorhabditis elegans / Q000235:genetics / Q000378:metabolism*; D029742:Caenorhabditis elegans Proteins / Q000235:genetics / Q000378:metabolism; D006152:Cyclic GMP / Q000378:metabolism*; D015536:Down-Regulation; D051858:Forkhead Transcription Factors / Q000235:genetics / Q000378:metabolism; D019206:GTP-Binding Protein alpha Subunits, Gi-Go / Q000235:genetics / Q000378:metabolism; D007328:Insulin / Q000378:metabolism*; D061385:Insulins; D008136:Longevity; D018384:Oxidative Stress; D036361:Peptide Hormones / Q000235:genetics / Q000378:metabolism; D011972:Receptor, Insulin / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D014158:Transcription, Genetic; D016212:Transforming Growth Factor beta / Q000378:metabolism
chemical_list: D029742:Caenorhabditis elegans Proteins; D051858:Forkhead Transcription Factors; C525653:Ins-7 protein, C elegans; D007328:Insulin; D061385:Insulins; D036361:Peptide Hormones; D014157:Transcription Factors; D016212:Transforming Growth Factor beta; C108831:daf-16 protein, C elegans; C473608:daf-28 protein, C elegans; C501263:gpa-3 protein, C elegans; D011972:Receptor, Insulin; D019206:GTP-Binding Protein alpha Subunits, Gi-Go; D006152:Cyclic GMP