title: Activation of intracellular signaling pathways by the murine cytomegalovirus G protein-coupled receptor M33 occurs via PLC-{beta}/PKC-dependent and -independent mechanisms.
abstract: The presence of numerous G protein-coupled receptor (GPCR) homologs within the herpesvirus genomes suggests an essential role for these genes in viral replication in the infected host. Such is the case for murine cytomegalovirus (MCMV), where deletion of the M33 GPCR or replacement of M33 with a signaling defective mutant has been shown to severely attenuate replication in vivo. In the present study we utilized a genetically altered version of M33 (termed R131A) in combination with pharmacological inhibitors to further characterize the mechanisms by which M33 activates downstream signaling pathways. This R131A mutant of M33 fails to support salivary gland replication in vivo and, as such, is an important tool that can be used to examine the signaling activities of M33. We show that M33 stimulates the transcription factor CREB via heterotrimeric G(q/11) proteins and not through promiscuous coupling of M33 to the G(s) pathway. Using inhibitors of signaling molecules downstream of G(q/11), we demonstrate that M33 stimulates CREB transcriptional activity in a phospholipase C-beta and protein kinase C (PKC)-dependent manner. Finally, utilizing wild-type and R131A versions of M33, we show that M33-mediated activation of other signaling nodes, including the mitogen-activated protein kinase family member p38alpha and transcription factor NF-kappaB, occurs in the absence of G(q/11) and PKC signaling. The results from the present study indicate that M33 utilizes multiple mechanisms to modulate intracellular signaling cascades and suggest that signaling through PLC-beta and PKC plays a central role in MCMV pathogenesis in vivo.
authors.affiliation: Department of Molecular Genetics, Biochemistry, and Microbiology, University of Cincinnati College of Medicine, OH 45267-0524, USA.
authors.forename: Joseph D
authors.initials: JD
authors.lastname: Sherrill
authors.forename: Melissa P
authors.initials: MP
authors.lastname: Stropes
authors.forename: Olivia D
authors.initials: OD
authors.lastname: Schneider
authors.forename: Diana E
authors.initials: DE
authors.lastname: Koch
authors.forename: Fabiola M
authors.initials: FM
authors.lastname: Bittencourt
authors.forename: Jeanette L C
authors.initials: JL
authors.lastname: Miller
authors.forename: William E
authors.initials: WE
authors.lastname: Miller
mesh_terms: D000818:Animals; D002460:Cell Line; D005260:Female; D006566:Herpesviridae Infections / Q000201:enzymology / Q000378:metabolism* / Q000821:virology; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D018146:Muromegalovirus / Q000235:genetics / Q000502:physiology*; D054799:Phospholipase C beta / Q000235:genetics / Q000378:metabolism*; D011493:Protein Kinase C / Q000235:genetics / Q000378:metabolism*; D043562:Receptors, G-Protein-Coupled / Q000235:genetics / Q000378:metabolism*; D012469:Salivary Glands / Q000201:enzymology / Q000378:metabolism / Q000821:virology; D015398:Signal Transduction; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism*; D014779:Virus Replication
chemical_list: D043562:Receptors, G-Protein-Coupled; D014764:Viral Proteins; D011493:Protein Kinase C; D054799:Phospholipase C beta