title: Sequence, expression divergence, and complementation of homologous ALCATRAZ loci in Brassica napus.
abstract: The genomic era provides new perspectives in understanding polyploidy evolution, mostly on the genome-wide scale. In this paper, we show the sequence and expression divergence between the homologous ALCATRAZ (ALC) loci in Brassica napus, responsible for silique dehiscence. We cloned two homologous ALC loci, namely BnaC.ALC.a and BnaA.ALC.a in B. napus. Driven by the 35S promoter, both the loci complemented to the alc mutation of Arabidopsis thaliana, yet only the expression of BnaC.ALC.a was detectable in the siliques of B. napus. Sequence alignment indicated that BnaC.ALC.a and BolC.ALC.a, or BnaA.ALC.a and BraA.ALC.a, possess a high level of similarity. The understanding of the sequence and expression divergence among homologous loci of a gene is of due importance for an effective gene manipulation and TILLING (or ECOTILLING) analysis for the allelic DNA variation at a given locus.
authors.affiliation: Institute of Crop Science, College of Agriculture and Biotechnology, Zhejiang University, 268 Kaixuan Road, 310029, Hangzhou, People's Republic of China.
authors.forename: Shuijin
authors.initials: S
authors.lastname: Hua
authors.forename: Imran Haider
authors.initials: IH
authors.lastname: Shamsi
authors.forename: Yuan
authors.initials: Y
authors.lastname: Guo
authors.forename: Haksong
authors.initials: H
authors.lastname: Pak
authors.forename: Mingxun
authors.initials: M
authors.lastname: Chen
authors.forename: Congguang
authors.initials: C
authors.lastname: Shi
authors.forename: Huabing
authors.initials: H
authors.lastname: Meng
authors.forename: Lixi
authors.initials: L
authors.lastname: Jiang
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D029688:Brassica napus / Q000235:genetics*; D017343:Genes, Plant; D005816:Genetic Complementation Test; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000235:genetics*; D011123:Polyploidy; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid
chemical_list: D010940:Plant Proteins