title: Detection of retinal metabolic stress resulting from central serous retinopathy.
abstract: OBJECTIVE
  To test whether eyes with central serous retinopathy have elevated retinal flavoprotein fluorescence (FPF) using a novel clinical imaging method.
  
  
  METHODS
  Three male patients with unilateral central serous retinopathy were examined for FPF at 535 nm induced by 1-msec flashes of 467 nm light. FPF was captured with an electron multiplying charged-coupled device camera with a 512 x 512 pixel chip. Average intensity of retinal FPF for each affected eye was compared with the contralateral, unaffected eye and with six age-matched control eyes by analyzing histograms of pixel intensities plotted for each eye.
  
  
  RESULTS
  For each patient, the central serous retinopathy-affected eye had a significantly greater retinal FPF when compared with the retinal FPF of the unaffected eye. Eyes affected with central serous retinopathy had retinal FPF values that averaged 98% greater than the retinal FPF of age-matched control eyes.
  
  
  CONCLUSIONS
  Flavoprotein fluorescence analysis may be useful for rapidly and noninvasively identifying metabolic tissue stress of central serous retinopathy.
authors.affiliation: Department of Ophthalmology and Visual Sciences, University of Michigan, Ann Arbor, Michigan 48105, USA.
authors.forename: Matthew G
authors.initials: MG
authors.lastname: Field
authors.forename: Victor M
authors.initials: VM
authors.lastname: Elner
authors.forename: Seung
authors.initials: S
authors.lastname: Park
authors.forename: Richard
authors.initials: R
authors.lastname: Hackel
authors.forename: John R
authors.initials: JR
authors.lastname: Heckenlively
authors.forename: Susan G
authors.initials: SG
authors.lastname: Elner
authors.forename: Howard R
authors.initials: HR
authors.lastname: Petty
mesh_terms: D000328:Adult; D015415:Biomarkers; D056833:Central Serous Chorioretinopathy / Q000378:metabolism* / Q000473:pathology / Q000503:physiopathology; D005420:Flavoproteins / Q000032:analysis / Q000378:metabolism; D005451:Fluorescein Angiography; D005453:Fluorescence; D006801:Humans; D008297:Male; D018384:Oxidative Stress; D015203:Reproducibility of Results; D012160:Retina / Q000378:metabolism* / Q000473:pathology / Q000503:physiopathology
chemical_list: D015415:Biomarkers; D005420:Flavoproteins