Development of a multiplex real-time PCR for detection and differentiation of malignant catarrhal fever viruses in clinical samples.
A multiplex real-time PCR was developed using a single pair of primers and fluorescent probes specific for five malignant catarrhal fever viruses and an internal positive control. The assay was able to simultaneously detect and differentiate the viruses in clinical samples with high sensitivity (97.2%) and specificity (100%).
Cunha
Cristina W
CW
USDA-ARS, Washington State University, Pullman, 99164-6630, USA. cwcunha@vetmed.wsu.edu
Otto
Lisa
L
Taus
Naomi S
NS
Knowles
Donald P
DP
Li
Hong
H
D000818:Animals; D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics; D006564:Herpesviridae / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D006566:Herpesviridae Infections / Q000175:diagnosis / Q000662:veterinary*; D006801:Humans; D008232:Lymphoproliferative Disorders / Q000175:diagnosis / Q000662:veterinary*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*; D012418:Ruminants / Q000821:virology*; D012680:Sensitivity and Specificity; D016415:Sequence Alignment
D017931:DNA Primers