title: Impaired in vivo vasculogenic potential of endothelial progenitor cells in comparison to human umbilical vein endothelial cells in a spheroid-based implantation model.
abstract: OBJECTIVE
  Neovascularization represents a major challenge in tissue engineering applications since implantation of voluminous grafts without sufficient vascularity results in hypoxic cell death of implanted cells. An attractive therapeutic approach to overcome this is based on co-implantation of endothelial cells to create vascular networks. We have investigated the potential of human endothelial progenitor cells (EPC) to form functional blood vessels in vivo in direct comparison to vascular-derived endothelial cells, represented by human umbilical vein endothelial cells (HUVEC).
  
  
  METHODS
  EPCs were isolated from human peripheral blood, expanded in vitro and analysed in vitro for phenotypical and functional parameters. In vivo vasculogenic potential of EPCs and HUVECs was evaluated in a xenograft model where spheroidal endothelial aggregates were implanted subcutaneously into immunodeficient mice.
  
  
  RESULTS
  EPCs were indistinguishable from HUVECs in terms of expression of classical endothelial markers CD31, von Willebrand factor, VE-cadherin and vascular endothelial growth factor-R2, and in their ability to endocytose acetylated low-density lipoprotein. Moreover, EPCs and HUVECs displayed almost identical angiogenic potential in vitro, as assessed by in vitro Matrigel sprouting assay. However in vivo, a striking and unexpected difference between EPCs and HUVECs was detected. Whereas implanted HUVEC spheroids gave rise to formation of a stable network of perfused microvessels, implanted EPC spheroids showed significantly impaired ability to form vascular structures under identical experimental conditions.
  
  
  CONCLUSIONS
  Our results indicate that vascular-derived endothelial cells, such as HUVECs are superior to EPCs in terms of promoting in vivo vascularization of engineered tissues.
authors.affiliation: Department of Plastic and Hand Surgery, University of Freiburg Medical Center, Freiburg, Germany. guenter.finkenzeller@uniklinik-freiburg.de
authors.forename: G
authors.initials: G
authors.lastname: Finkenzeller
authors.forename: S
authors.initials: S
authors.lastname: Graner
authors.forename: C J
authors.initials: CJ
authors.lastname: Kirkpatrick
authors.forename: S
authors.initials: S
authors.lastname: Fuchs
authors.forename: G B
authors.initials: GB
authors.lastname: Stark
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D042783:Endothelial Cells / Q000166:cytology* / Q000276:immunology / Q000637:transplantation; D006801:Humans; D051379:Mice; D016513:Mice, SCID; D018919:Neovascularization, Physiologic; D010641:Phenotype; D018874:Spheroids, Cellular / Q000166:cytology / Q000637:transplantation*; D033581:Stem Cell Transplantation; D013234:Stem Cells / Q000166:cytology* / Q000276:immunology; D023822:Tissue Engineering; D014183:Transplantation, Heterologous; D014471:Umbilical Veins / Q000166:cytology*