title: Non-Hodgkin lymphoma (NHL) subtypes defined by common translocations: utility of fluorescence in situ hybridization (FISH) in a case-control study.
abstract: We used fluorescence in situ hybridization (FISH) assays to identify t(14;18) translocations in archival paraffin-embedded tumor sections from non-Hodgkin lymphoma (NHL) cases enrolled in a population-based study. t(14;18) was identified in 54% of 152 cases, including 39% of diffuse large cell lymphomas (26 of 66 cases) and 84% of follicular lymphomas (36 of 43 cases). Eighty-seven percent of t(14;18)-positive cases and 57% of t(14;18)-negative cases expressed bcl-2. FISH assays detected twice as many t(14;18)-positive follicular lymphomas as PCR assays. Overall, study findings support the use of FISH assays to detect t(14;18) in archival tumor samples for epidemiologic studies of NHL subtypes.
authors.affiliation: Infections and Immunoepidemiology Branch, Division of Cancer Epidemiology & Genetics, National Cancer Institute, National Institutes of Health, 6120 Executive Blvd., EPS 7074, Rockville, MD 20892, USA. changcm@mail.nih.gov
authors.forename: Cindy M
authors.initials: CM
authors.lastname: Chang
authors.forename: Jane C
authors.initials: JC
authors.lastname: Schroeder
authors.forename: Wen-Yi
authors.initials: WY
authors.lastname: Huang
authors.forename: Cherie H
authors.initials: CH
authors.lastname: Dunphy
authors.forename: Ralph S
authors.initials: RS
authors.lastname: Baric
authors.forename: Andrew F
authors.initials: AF
authors.lastname: Olshan
authors.forename: Kathleen C
authors.initials: KC
authors.lastname: Dorsey
authors.forename: Georgette A
authors.initials: GA
authors.lastname: Dent
authors.forename: James R
authors.initials: JR
authors.lastname: Cerhan
authors.forename: Charles F
authors.initials: CF
authors.lastname: Lynch
authors.forename: Nathaniel
authors.initials: N
authors.lastname: Rothman
authors.forename: Kenneth P
authors.initials: KP
authors.lastname: Cantor
authors.forename: Aaron
authors.initials: A
authors.lastname: Blair
mesh_terms: D016022:Case-Control Studies; D002883:Chromosomes, Human, Pair 14; D002887:Chromosomes, Human, Pair 18; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods* / Q000592:standards; D008224:Lymphoma, Follicular / Q000235:genetics*; D016403:Lymphoma, Large B-Cell, Diffuse / Q000235:genetics*; D008228:Lymphoma, Non-Hodgkin / Q000145:classification* / Q000235:genetics*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000032:analysis; D014178:Translocation, Genetic
chemical_list: D019253:Proto-Oncogene Proteins c-bcl-2