Sodium caseinate induces secretion of macrophage colony-stimulating factor from neutrophils.
In this work we provide evidence that granulocytes produce macrophage colony-stimulating factor (M-CSF) in the band cell stage and secrete it upon sodium caseinate-mediated differentiation to polymorphonuclear cells. We identified M-CSF in an enriched population of myeloid band cells from murine bone marrow using a chromophore-labeled monoclonal anti-M-CSF antibody. An ELISA assay was then used to detect secreted M-CSF in culture supernatants of enriched band cells differentiated to mature neutrophils using sodium caseinate. Colony formation in vitro by the supernatants from differentiating band cells was blocked by anti-M-CSF, thus suggesting that this factor is the only one responsible for this activity. Our data imply that casein can modulate hematopoiesis possibly via M-CSF production. Finally we discuss the possibility whether this M-CSF in concert with G-CSF could establish a cellular communication network between macrophages and granulocytes allowing them to simultaneously arrive at the inflammatory site.
Santiago-Osorio
E
E
Laboratorio de Hematopoyesis y Leucemia, Laboratorio 8, PB, UMIEZ. Campus II. Facultad de Estudios Superiores Zaragoza, UNAM, Batalla 5 de Mayo s/n, Col. E. Oriente, Esquina Fuerte Loreto, Iztapalapa, CP 09230, México, DF., México. edelmiro@servidor.unam.mx
Mora
L
L
Bautista
M
M
Montesinos
J J
JJ
Martínez
I
I
Ramos-Mandujano
G
G
Zambrano
R
R
Monroy-García
A
A
Weiss-Steider
B
B
Ledesma-Martínez
E
E
Aguiñiga
I
I
D000818:Animals; D001854:Bone Marrow Cells / Q000187:drug effects / Q000502:physiology; D002364:Caseins / Q000494:pharmacology*; D002454:Cell Differentiation; D005260:Female; D016173:Macrophage Colony-Stimulating Factor / Q000378:metabolism*; D008297:Male; D051379:Mice; D009504:Neutrophils / Q000187:drug effects* / Q000378:metabolism
D002364:Caseins; D016173:Macrophage Colony-Stimulating Factor