title: The effect of microwave irradiation on DNA hybridization.
abstract: The effect of microwave irradiation on DNA/DNA hybridization has been studied under controlled power and temperature conditions. It was discovered that microwave irradiation led to the melting of double-stranded deoxyoligonucleotides well below their thermal melting temperature and independent of the length of the deoxyoligonucleotides. These observations indicate a specific interaction of microwaves with DNA, and have important implications in the chemical or enzymatic processing of DNA under microwave heating.
authors.affiliation: Department of Chemistry, North Carolina State University, Raleigh, NC 27695-8204, USA.
authors.forename: Wesleigh F
authors.initials: WF
authors.lastname: Edwards
authors.forename: Douglas D
authors.initials: DD
authors.lastname: Young
authors.forename: Alexander
authors.initials: A
authors.lastname: Deiters
mesh_terms: D001483:Base Sequence; D004247:DNA / Q000737:chemistry* / Q000235:genetics / Q000528:radiation effects*; D008872:Microwaves; D009691:Nucleic Acid Denaturation / Q000528:radiation effects; D009693:Nucleic Acid Hybridization / Q000528:radiation effects; D013050:Spectrometry, Fluorescence; D044366:Transition Temperature
chemical_list: D004247:DNA