[In vitro analysis of intake of minocycline by mature rat mandibular osteoblasts].
OBJECTIVE
To observe the intake of minocycline and its amount in mature rat mandibular osteoblasts (MRMOB) in vitro, and to identify the feasibility of intracellular anti-bacterial activity of minocycline.


METHODS
Four groups of MRMOB were incubated in 100 mg/L minocycline for 15, 30, 45 and 60 minutes respectively, and the accumulation of minocycline within MRMOB was measured using a fluorescence spectrophotometer.


RESULTS
The intracellular accumulation amount of minocycline in the four groups of MRMOB was (17.29 +/- 1.49), (16.87 +/- 1.57), (16.96 +/- 1.67) and (17.94 +/- 1.63) mg/g respectively after osteoblasts were cultured in Dulbecco's modified Eagle's medium (DMEM) which contained minocycline for 15, 30, 45 and 60 min. There was no significant difference in amount of minocycline among the four groups of MRMOB.


CONCLUSIONS
The mature rat mandibular osteoblasts can ingest minocycline, and the accumulation amount of minocycline in MRMOB is irrelevant with the exposure time of MRMOB to minocycline.
Zhang
Wen-Yi
WY
Stomatological Institute, General Hospital of PLA, Beijing 100853, China.
Liu
Hong-Chen
HC
Wu
Xia
X
E
Ling-Ling
LL
Lü
Yan
Y
Zhang
Jian
J
D000818:Animals; D002478:Cells, Cultured; D008334:Mandible / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D008911:Minocycline / Q000493:pharmacokinetics*; D010006:Osteoblasts / Q000187:drug effects* / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley
D008911:Minocycline