title: Antibacterial properties and mode of action of a short acyl-lysyl oligomer.
abstract: We investigated the potency, selectivity, and mode of action of the oligo-acyl-lysine (OAK) NC(12)-2 beta(12), which was recently suggested to represent the shortest OAK sequence that retains nonhemolytic antibacterial properties. A growth inhibition assay against a panel of 48 bacterial strains confirmed that NC(12)-2 beta(12) exerted potent activity against gram-positive bacteria while exhibiting negligible hemolysis up to at least 100 times the MIC. Interestingly, NC(12)-2 beta(12) demonstrated a bacteriostatic mode of action, unlike previously described OAKs that were bactericidal and essentially active against gram-negative bacteria only. The results of various experiments with binding to model phospholipid membranes correlated well with those of the cytotoxicity experiments and provided a plausible explanation for the observed activity profile. Thus, surface plasmon resonance experiments performed with model bilayers revealed high binding affinity to a membrane composition that mimicked the plasma membrane of staphylococci (global affinity constant [K(app)], 3.7 x 10(6) M(-1)) and significantly lower affinities to mimics of Escherichia coli or red blood cell cytoplasmic membranes. Additional insertion isotherms and epifluorescence microscopy experiments performed with model Langmuir monolayers mimicking the outer leaflet of plasma membranes demonstrated the preferential insertion of NC(12)-2 beta(12) into highly anionic membranes. Finally, we provide mechanistic studies in support of the view that the bacteriostatic effect resulted from a relatively slow process of plasma membrane permeabilization involving discrete leakage of small solutes, such as intracellular ATP. Collectively, the data point to short OAKs as a potential source for new antibacterial compounds that can selectively affect the growth of gram-positive bacteria while circumventing potential adverse effects linked to lytic compounds.
authors.affiliation: Department of Biotechnology and Food Engineering, Technion-Israel Institute of Technology, Haifa, Israel.
authors.forename: Fadia
authors.initials: F
authors.lastname: Zaknoon
authors.forename: Hadar
authors.initials: H
authors.lastname: Sarig
authors.forename: Shahar
authors.initials: S
authors.lastname: Rotem
authors.forename: Liran
authors.initials: L
authors.lastname: Livne
authors.forename: Andrey
authors.initials: A
authors.lastname: Ivankin
authors.forename: David
authors.initials: D
authors.lastname: Gidalevitz
authors.forename: Amram
authors.initials: A
authors.lastname: Mor
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology*; D023181:Antimicrobial Cationic Peptides / Q000737:chemistry*; D002462:Cell Membrane / Q000737:chemistry; D004912:Erythrocytes / Q000737:chemistry / Q000187:drug effects; D004926:Escherichia coli / Q000737:chemistry / Q000187:drug effects*; D006094:Gram-Positive Bacteria / Q000737:chemistry / Q000187:drug effects*; D006461:Hemolysis / Q000187:drug effects; D006801:Humans; D008856:Microscopy, Fluorescence; D013210:Staphylococcus / Q000737:chemistry / Q000187:drug effects; D020349:Surface Plasmon Resonance
chemical_list: D000900:Anti-Bacterial Agents; D023181:Antimicrobial Cationic Peptides