[Comparative study of five techniques of preparation of platelet-rich plasma].
OBJECTIVE
Injections of platelet-rich plasma (PRP) constitute a new therapeutic for treating chronic tendinopathies. The injection being carried out in the tendon, the volume of PRP should thus be minimal (to decrease the intratendinous pressure and to minimize pain). This PRP should also have a raised platelet count. The quantity of released growth factors could be related to the system of preparation employed. We thus carried out a comparative study of five techniques of preparation of PRP described in the literature.


METHODS
Samples of venous blood were taken among five patients in order to compare five techniques of preparation of PRP: University Hospital of Liège technique, Curasan(®) PRP Kit, Plateltex(®), GPS(®)II and RegenLab(®).


RESULTS
The various techniques make it possible to obtain more important platelet concentration than in blood, with variable volumes (0,3 to 6ml). The number of platelets per microlitre appears higher with Plateltex(®) and obtains smallest volume of PRP. The other techniques also give small volumes except for the GPS(®)II. The number of collected platelets with this technique appears thus higher. The best collect efficiency is obtained with RegenLab(®).


CONCLUSIONS
The technique Plateltex(®) makes it possible to collect the highest concentration of platelets in the smallest volume available.
Kaux
J-F
JF
Médecine de l'appareil locomoteur et traumatologie du sport, université de Liège, CHU Sart-Tilman, avenue de l'Hôpital, B35, 4000 Liège, Belgique.
Le Goff
C
C
Seidel
L
L
Péters
P
P
Gothot
A
A
Albert
A
A
Crielaard
J-M
JM
D000328:Adult; D000925:Anticoagulants / Q000494:pharmacology; D001781:Blood Component Removal / Q000379:methods*; D002498:Centrifugation; D019343:Citric Acid / Q000494:pharmacology; D004906:Erythrocyte Count; D005260:Female; D005947:Glucose / Q000031:analogs & derivatives / Q000494:pharmacology; D006400:Hematocrit; D006801:Humans; D007958:Leukocyte Count; D008297:Male; D008875:Middle Aged; D010976:Platelet Count; D053657:Platelet-Rich Plasma; D013048:Specimen Handling / Q000295:instrumentation / Q000379:methods; D052256:Tendinopathy / Q000628:therapy; D055815:Young Adult
D000925:Anticoagulants; C002113:acid citrate dextrose; D019343:Citric Acid; D005947:Glucose