title: Low copy number and high 4977 deletion of mitochondrial DNA in uterosacral ligaments are associated with pelvic organ prolapse progression.
abstract: OBJECTIVE
  The pathophysiology of pelvic organ prolapse (POP) is related to aging in the pelvic organ support, and mitochondrial dysfunction is one of the major contributors to aging. Therefore, the objective of this study was to investigate the correlation between alternations of mitochondrial DNA and progression of POP.
  
  
  METHODS
  Polymerase chain reaction (PCR) was applied in the present study. Uterosacral ligaments (UL) were obtained from 45 POP patients and 38 myoma patients without POP. Chi-square test, Student's t-test, Mann-Whitney U test, and Spearman correlation analysis were applied in the comparison between POP and non-POP patients.
  
  
  RESULTS
  The results revealed that significant depletion of mitochondrial DNA (mtDNA) and an increase in the incidence of 4977 deletion of mtDNA (mtDNA(4977)) in the UL tissue of POP patients.
  
  
  CONCLUSIONS
  The alternations of mtDNA may play an important role in the molecular pathogenesis and process of POP formation.
authors.affiliation: Department of Obstetrics and Gynecology, Changhua Christian Hospital, Changhua, Taiwan.
authors.forename: Mou-Jong
authors.initials: MJ
authors.lastname: Sun
authors.forename: Wen-Ling
authors.initials: WL
authors.lastname: Cheng
authors.forename: Yau-Huei
authors.initials: YH
authors.lastname: Wei
authors.forename: Chen-Ling
authors.initials: CL
authors.lastname: Kuo
authors.forename: Samuel
authors.initials: S
authors.lastname: Sun
authors.forename: Horng-Der
authors.initials: HD
authors.lastname: Tsai
authors.forename: Hui-Mei
authors.initials: HM
authors.lastname: Lin
authors.forename: Chin-San
authors.initials: CS
authors.lastname: Liu
mesh_terms: D000328:Adult; D000368:Aged; D000375:Aging / Q000378:metabolism; D001706:Biopsy; D016022:Case-Control Studies; D004272:DNA, Mitochondrial / Q000235:genetics* / Q000378:metabolism*; D018450:Disease Progression; D005260:Female; D017353:Gene Deletion; D018628:Gene Dosage / Q000235:genetics*; D006801:Humans; D007889:Leiomyoma; D008022:Ligaments / Q000378:metabolism* / Q000473:pathology / Q000503:physiopathology; D008875:Middle Aged; D008928:Mitochondria / Q000378:metabolism*; D018908:Muscle Weakness / Q000378:metabolism / Q000503:physiopathology; D009214:Myoma; D012447:Sacrum; D014594:Uterine Neoplasms; D014596:Uterine Prolapse / Q000209:etiology / Q000378:metabolism* / Q000503:physiopathology; D014599:Uterus
chemical_list: D004272:DNA, Mitochondrial