title: The cis-regulatory sequences required for expression of the Drosophila melanogaster adult cuticle gene ACP65A.
abstract: Post-embryonic development in insects requires successive molts. Molts are triggered by ecdysteroids, and the nature of the molt (larval, pupal or adult) is determined by juvenile hormones. The genes encoding cuticle proteins are targets of both classes of hormones, and therefore are interesting models to study hormone action at the molecular level. The Drosophila ACP65A cuticle gene is expressed exclusively during the synthesis of the adult exoskeleton, in epidermal domains synthesising flexible cuticle. We have examined the cis-regulatory sequences of ACP65A using phylogenetic comparisons and functional analysis, and find that only about 180 bp are essential, including an 81 bp intron. The restriction of ACP65A expression appears to depend on a strong repression mechanism.
authors.affiliation: UMR CNRS 5548 Développement-Communication Chimique, Université de Bourgogne, 21000 Dijon, France.
authors.forename: M
authors.initials: M
authors.lastname: Lestradet
authors.forename: E
authors.initials: E
authors.lastname: Gervasio
authors.forename: S
authors.initials: S
authors.lastname: Fraichard
authors.forename: S
authors.initials: S
authors.lastname: Dupas
authors.forename: J
authors.initials: J
authors.lastname: Alabouvette
authors.forename: A
authors.initials: A
authors.lastname: Lemoine
authors.forename: J P
authors.initials: JP
authors.lastname: Charles
mesh_terms: D000818:Animals; D001483:Base Sequence; D004331:Drosophila melanogaster / Q000502:physiology*; D005786:Gene Expression Regulation / Q000502:physiology*; D019476:Insect Proteins / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D010802:Phylogeny; D050436:Regulatory Elements, Transcriptional; D012045:Regulatory Sequences, Nucleic Acid
chemical_list: D019476:Insect Proteins; C047663:cuticle proteins, insects