Dependence of swarming in Escherichia coli K-12 on spermidine and the spermidine importer.
In a previous work, it was observed that the swarming of polyamine-deficient Proteus mirabilis (speB::sm) was severely inhibited on Luria-Bertani (LB) swarming plates (LBSw) (LB, 0.5% glucose, 0.5% agar), and it was clarified that extracellular putrescine was important as a signaling molecule for the induction of swarming in P. mirabilis. However, a polyamine-deficient strain (delta-speAB delta-speC) of Escherichia coli swarmed as well as the parental strain on LBSw plates. We report that the swarming phenotype of a polyamine-deficient E. coli strain is dependent on spermidine and PotABCD, a spermidine importer.
Kurihara
Shin
S
Japan Collection of Microorganisms, Microbe Division, RIKEN BioResource Center, Wako, Saitama, Japan. kuri711@jcm.riken.jp
Suzuki
Hideyuki
H
Tsuboi
Yuichi
Y
Benno
Yoshimi
Y
D018528:ATP-Binding Cassette Transporters / Q000235:genetics / Q000378:metabolism*; D048168:Escherichia coli K12 / Q000502:physiology*; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism*; D017353:Gene Deletion; D008124:Locomotion; D026901:Membrane Transport Proteins / Q000235:genetics / Q000378:metabolism*; D033902:Periplasmic Binding Proteins / Q000235:genetics / Q000378:metabolism*; D013095:Spermidine / Q000378:metabolism*
D018528:ATP-Binding Cassette Transporters; D029968:Escherichia coli Proteins; D026901:Membrane Transport Proteins; D033902:Periplasmic Binding Proteins; C096374:PotA protein, E coli; C096375:PotB protein, E coli; C096376:PotC protein, E coli; C099274:PotD protein, E coli; D013095:Spermidine