Effect of gamma-irradiation on agaritine, gamma-glutaminyl-4-hydroxybenzene (GHB), antioxidant capacity, and total phenolic content of mushrooms ( Agaricus bisporus ).
Fresh mushrooms ( Agaricus bisporus ) were irradiated at doses of 1, 3, and 5 kGy to assess the effect of gamma-irradiation on the major aromatic compounds agaritine (beta-N-(gamma-L-(+)-glutamyl)-4-(hydroxymethyl)phenylhydrazine) and GHB (gamma-glutaminyl-4-hydroxybenzene) as well as on the total phenolic content and antioxidant capacity. Up to 3 kGy, agaritine was not affected. At 5 kGy, a significant reduction (p = 0.05) from 1.54 (0 kGy) to 1.35 g/kg dry weight (DW) was observed. gamma-Glutaminyl-4-hydroxybenzene decreased by 22% at 1 kGy and by 31% at 5 kGy. Additionally, agaritine standard solutions at concentrations of 10(-4) and 5 x 10(-5) mol/L were irradiated to compare the effect on agaritine content in aqueous solutions and in the sample matrix. A rapid decay was observed, 50% at 750 Gy (10(-4) mol/L) and 400 Gy (5 x 10(-5) mol/L). The total phenolic content and antioxidant capacity were not significantly (p = 0.05) influenced by irradiation.
Sommer
Isolde
I
Department of Nutritional Sciences, University of Vienna, Althanstrasse 14, A-1090 Vienna, Austria.
Schwartz
Heidi
H
Solar
Sonja
S
Sontag
Gerhard
G
D000364:Agaricus / Q000737:chemistry* / Q000528:radiation effects; D000975:Antioxidants / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D004307:Dose-Response Relationship, Radiation; D005514:Food Irradiation / Q000009:adverse effects*; D005720:Gamma Rays; D005973:Glutamine / Q000031:analogs & derivatives* / Q000737:chemistry / Q000528:radiation effects; D010636:Phenols / Q000032:analysis*; D010659:Phenylhydrazines / Q000737:chemistry / Q000528:radiation effects*; D021241:Spectrometry, Mass, Electrospray Ionization
D000975:Antioxidants; D010636:Phenols; D010659:Phenylhydrazines; D005973:Glutamine; C001298:gamma-L-glutaminyl-4-hydroxybenzene; C015654:agaritine