title: Rapid qualitative and quantitative analysis of opiates in extract of poppy head via FTIR and chemometrics: towards in-field sensors.
abstract: Identification and quantification of the opiates morphine and thebaine has been achieved in three commercial poppy cultivars using FTIR-ATR spectroscopy, from a simple and rapid methanolic extraction, suitable for field analysis. The limits of detection were 0.13 mg/ml (0.013%, w/v) and 0.3 mg/ml (0.03%, w/v) respectively. The concentrations of opiates present were verified with HPLC-MS. The chemometrics has been used to identify specific "signature" peaks in the poppy IR spectra for characterisation of cultivar by its unique fingerprint offering a potential forensic application in opiate crop analysis.
authors.affiliation: Cranfield University, Cranfield, Bedfordshire, UK. nicholas.turner@newcastle.edu.au
authors.forename: Nicholas W
authors.initials: NW
authors.lastname: Turner
authors.forename: Michael
authors.initials: M
authors.lastname: Cauchi
authors.forename: Elena V
authors.initials: EV
authors.lastname: Piletska
authors.forename: Christopher
authors.initials: C
authors.lastname: Preston
authors.forename: Sergey A
authors.initials: SA
authors.lastname: Piletsky
mesh_terms: D000465:Algorithms; D000701:Analgesics, Opioid / Q000032:analysis*; D015374:Biosensing Techniques / Q000379:methods*; D020650:Combinatorial Chemistry Techniques / Q000379:methods*; D010207:Papaver / Q000737:chemistry*; D010936:Plant Extracts / Q000032:analysis*; D012680:Sensitivity and Specificity; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods*
chemical_list: D000701:Analgesics, Opioid; D010936:Plant Extracts