Effect of follicular wave synchronization on in vitro embryo production in heifers.
Aiming to achieve the ideal time of ovum pick-up (OPU) for in vitro embryo production (IVP) in crossbred heifers, two Latin square design studies investigated the effect of ovarian follicular wave synchronization with estradiol benzoate (EB) and progestins. For each experiment, crossbred heifers stage of estrous cycle was synchronized either with a norgestomet ear implant (Experiment 1) or a progesterone intravaginal device (Experiment 2) for 7d, followed by the administration of 150microg d-cloprostenol. On Day 7, all follicles >3mm in diameter were aspirated and implants/devices were replaced by new ones. Afterwards, implant/device replacement was conducted every 14d. Each experiment had three treatment groups. In Experiment 1 (n=12), heifers in Group 2X had their follicles aspirated twice a week and those in Groups 1X and 1X-EB were submitted to OPU once a week for a period of 28d. Heifers from Group 1X-EB also received 2mg EB i.m. immediately after each OPU session. In Experiment 2 (n=11), animals from Group 0EB did not receive EB while heifers in Groups 2EB and 5EB received 2 and 5mg of EB respectively, immediately after OPU. The OPU sessions were performed once weekly for 28d. Therefore, in both experiments, four OPU sessions were performed in heifers aspirated once a week and in Experiment 1, eight OPU sessions were done in heifers aspirated twice a week. Additionally, during the 7-d period following follicular aspiration, ovarian ultrasonography examinations were conducted to measure diameter of the largest follicle and blood samples were collected for FSH quantification by RIA. In Experiment 1, all viable oocytes recovered were in vitro matured and fertilized. Results indicated that while progestin and EB altered follicular wave patterns, this treatment did not prevent establishment of follicular dominance on the ovaries of heifers during OPU at 7-d intervals. Furthermore, the proposed stage of follicular wave synchronization strategies did not improve the number and quality of the recovered oocytes, or the number of in vitro produced embryos.
Ramos
A F
AF
Veterinary School of Federal University of Minas Gerais, Belo Horizonte, MG, Brazil. aleframos@cenargen.embrapa.br
Rumpf
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R
Câmara
J U
JU
Mollo
M R
MR
Pivato
I
I
Marques
A P
AP
Sartori
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D000818:Animals; D002417:Cattle; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D002970:Cleavage Stage, Ovum / Q000187:drug effects; D015237:Dinoprost / Q000008:administration & dosage; D004343:Drug Implants / Q000008:administration & dosage; D046149:Embryo Culture Techniques; D047108:Embryonic Development / Q000187:drug effects* / Q000502:physiology; D004958:Estradiol / Q000031:analogs & derivatives* / Q000494:pharmacology / Q000627:therapeutic use; D004973:Estrus Synchronization / Q000187:drug effects / Q000502:physiology*; D005260:Female; D005307:Fertilization in Vitro / Q000187:drug effects; D007273:Injections, Intramuscular; D054315:Oocyte Retrieval / Q000379:methods / Q000662:veterinary; D009865:Oocytes / Q000187:drug effects / Q000502:physiology; D006080:Ovarian Follicle / Q000000981:diagnostic imaging / Q000187:drug effects* / Q000502:physiology*; D011282:Pregnenediones / Q000008:administration & dosage / Q000494:pharmacology; D011372:Progestins / Q000494:pharmacology* / Q000627:therapeutic use; D011786:Quality Control; D014463:Ultrasonography
D004343:Drug Implants; D011282:Pregnenediones; D011372:Progestins; C074283:estradiol 3-benzoate; C100284:norgestomet; D004958:Estradiol; D015237:Dinoprost