Cloning, overproduction, purification, crystallization and preliminary X-ray diffraction analysis of yeast glutaredoxin Grx5.
Grx5 from the yeast Saccharomyces cerevisiae is a monothiol glutaredoxin that is involved in iron-sulfur cluster biogenesis. Here, yeast Grx5 was cloned and overproduced in Escherichia coli. The purified protein was crystallized using the hanging-drop vapour-diffusion method. Diffraction data for Grx5 were collected to 1.67 A resolution. The crystal of Grx5 belonged to space group R3, with unit-cell parameters a = b = 85.12, c = 48.95 A, alpha = beta = 90.00, gamma = 120.00 degrees .
Wang
Yi
Y
Hefei National Laboratory for Physical Sciences at Microscale and School of Life Sciences, University of Science and Technology of China, Hefei, Anhui 230026, People's Republic of China.
He
Yong Xing
YX
Yu
Jiang
J
Zhou
Cong Zhao
CZ
D003001:Cloning, Molecular; D003460:Crystallization; D004271:DNA, Fungal / Q000235:genetics; D003625:Data Collection; D004926:Escherichia coli / Q000235:genetics; D005822:Genetic Vectors; D054477:Glutaredoxins / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D010957:Plasmids; D012441:Saccharomyces cerevisiae / Q000737:chemistry*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*; D013223:Statistics as Topic; D013696:Temperature; D013698:Templates, Genetic; D013997:Time Factors; D014169:Transformation, Bacterial; D014961:X-Ray Diffraction
D004271:DNA, Fungal; D054477:Glutaredoxins; C528773:Grx5 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins