Enhanced electrochemiluminescence of CdSe quantum dots composited with CNTs and PDDA for sensitive immunoassay.
Electrochemiluminescence (ECL) of CdSe quantum dots (QDs) was greatly enhanced by the combination of carbon nanotubes (CNTs) and poly (diallyldimethylammonium chloride) (PDDA) in the CdSe QDs film, and could successfully be used to develop a sensitive ECL immunosensor for the detection of human IgG (Ag). The novel CdSe QDs-CNTs composites exhibited high ECL intensity, good biocompatibility, and high stability, which held great promise for the fabrication of the ECL biosensors with improved sensitivity. After PDDA as a binding linker was conjugated to the CdSe QDs-CNTs composite film on the electrode, the ECL signal was significantly enhanced. Subsequently, gold nanoparticles (GNPs) assembled onto the CdSe QDs-CNTs/PDDA modified electrode could amplify the ECL signal once again. After antibody (Ab) was immobilized onto the electrode through GNPs, the ECL immunosensor was successfully fabricated. It is for the first time that the unique function of PDDA for enhancing QDs ECL was explored and used to develop an ECL biosensor. The principle of ECL detection for target Ag is based on the increment of steric hindrance after immunoreaction, which resulted in the decrease of ECL intensity. The Ag concentration was determined in the linear range of 0.002-500 ng L(-1) with a detection limit of 0.6 pg mL(-1). The sensor showed good fabrication and detection reproducibility, and the assay results were in acceptable agreement with the clinical sera tests, showing a promising clinical application. This work opened the new avenues for applying QDs ECL in highly sensitive bioassays.
Jie
Guifen
G
Key Laboratory of Analytical Chemistry for Life Science (Ministry of Education of China), School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093, PR China.
Li
Lingling
L
Chen
Chao
C
Xuan
Jie
J
Zhu
Jun-Jie
JJ
D000911:Antibodies, Monoclonal / Q000737:chemistry / Q000276:immunology; D015374:Biosensing Techniques / Q000295:instrumentation*; D004563:Electrochemistry / Q000295:instrumentation; D004566:Electrodes; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006801:Humans; D007118:Immunoassay / Q000295:instrumentation*; D007074:Immunoglobulin G / Q000032:analysis* / Q000737:chemistry / Q000276:immunology; D008163:Luminescent Measurements / Q000295:instrumentation*; D037742:Nanotubes, Carbon / Q000737:chemistry* / Q000648:ultrastructure; D011095:Polyethylenes / Q000737:chemistry*; D045663:Quantum Dots; D000644:Quaternary Ammonium Compounds / Q000737:chemistry*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D014159:Transducers
D000911:Antibodies, Monoclonal; D007074:Immunoglobulin G; D037742:Nanotubes, Carbon; D011095:Polyethylenes; D000644:Quaternary Ammonium Compounds; C041004:poly-N,N-dimethyl-N,N-diallylammonium chloride