Rapid detection of human papilloma virus using a novel leaky surface acoustic wave peptide nucleic acid biosensor.
A novel leaky surface acoustic wave (LSAW) bis-peptide nucleic acid (bis-PNA) biosensor with double two-port resonators has been constructed successfully for the quantitative detection of human papilloma virus (HPV). The bis-PNA probe can directly detect HPV genomic DNA without polymerase chain reaction (PCR) amplification, and it can bind to the target DNA sequences more effectively and specifically than a DNA probe. When the concentrations varied from 1 pg/L to 1000 microg/L, with 100 microg/L being the optimal, a typical linearity was found between the quantity of target and the phase shifts. The detection limit was 1.21 pg/L and the clinical specificity was 97.22% of that of real-time PCR. The bis-PNA probe was able to distinguish sequences that differ only in one base. Both the intraassay and interassay coefficients of variance (CVs) were <10%, and the biosensor can be regenerated for ten times without appreciable loss of activity. Therefore, this technical platform of LSAW biosensor can be applied to clinical samples for direct HPV detection.
Wang
Yunxia
Y
Department of Laboratory Medicine, Southwest Hospital, Third Military Medical University, Chongqing 400038, PR China. wang52306@tom.com
Chen
Ming
M
Zhang
Liqun
L
Ding
Yi
Y
Luo
Yang
Y
Xu
Qinghua
Q
Shi
Jianfeng
J
Cao
Liang
L
Fu
Weiling
W
D000162:Acoustics / Q000295:instrumentation*; D015374:Biosensing Techniques / Q000295:instrumentation*; D004279:DNA, Viral / Q000235:genetics* / Q000302:isolation & purification; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006801:Humans; D027383:Papillomaviridae / Q000235:genetics* / Q000302:isolation & purification*; D020135:Peptide Nucleic Acids / Q000235:genetics* / Q000302:isolation & purification*
D004279:DNA, Viral; D020135:Peptide Nucleic Acids