title: Natriuretic peptides enhance the production of adiponectin in human adipocytes and in patients with chronic heart failure.
abstract: OBJECTIVE
  We investigated the functional relationship between natriuretic peptides and adiponectin by performing both experimental and clinical studies.
  
  
  BACKGROUND
  Natriuretic peptides are promising candidates for the treatment of congestive heart failure (CHF) because of their wide range of beneficial effects on the cardiovascular system. Adiponectin is a cytokine derived from adipose tissue with various cardiovascular-protective effects that has been reported to show a positive association with plasma brain natriuretic peptide (BNP) levels in patients with heart failure.
  
  
  METHODS
  The expression of adiponectin messenger ribonucleic acid (mRNA) and its secretion were examined after atrial natriuretic peptide (ANP) or BNP was added to primary cultures of human adipocytes in the presence or absence of HS142-1 (a functional type A guanylyl cyclase receptor antagonist). Changes of the plasma adiponectin level were determined in 30 patients with CHF who were randomized to receive intravenous ANP (0.025 microg/kg/min human ANP for 3 days, n = 15) or saline (n = 15).
  
  
  RESULTS
  Both ANP and BNP dose-dependently enhanced the expression of adiponectin mRNA and its secretion, whereas such enhancement was inhibited by pre-treatment with HS142-1. The plasma adiponectin level was increased at 4 days after administration of human ANP compared with the baseline value (from 6.56 +/- 0.40 microg/ml to 7.34 +/- 0.47 microg/ml, p < 0.05), whereas there was no change of adiponectin in the saline group (from 6.53 +/- 0.57 microg/ml to 6.55 +/- 0.56 microg/ml).
  
  
  CONCLUSIONS
  Natriuretic peptides enhance adiponectin production by human adipocytes in vitro and even in patients with CHF, which might have a beneficial effect on cardiomyocytes in patients receiving recombinant natriuretic peptide therapy for heart failure.
authors.affiliation: Cardiovascular Division of Medicine, National Cardiovascular Center, Suita, Osaka, Japan.
authors.forename: Osamu
authors.initials: O
authors.lastname: Tsukamoto
authors.forename: Masashi
authors.initials: M
authors.lastname: Fujita
authors.forename: Mahoto
authors.initials: M
authors.lastname: Kato
authors.forename: Satoru
authors.initials: S
authors.lastname: Yamazaki
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Asano
authors.forename: Akiko
authors.initials: A
authors.lastname: Ogai
authors.forename: Hidetoshi
authors.initials: H
authors.lastname: Okazaki
authors.forename: Mitsutoshi
authors.initials: M
authors.lastname: Asai
authors.forename: Yoko
authors.initials: Y
authors.lastname: Nagamachi
authors.forename: Norikazu
authors.initials: N
authors.lastname: Maeda
authors.forename: Yasunori
authors.initials: Y
authors.lastname: Shintani
authors.forename: Tetsuo
authors.initials: T
authors.lastname: Minamino
authors.forename: Masanori
authors.initials: M
authors.lastname: Asakura
authors.forename: Ichiro
authors.initials: I
authors.lastname: Kishimoto
authors.forename: Tohru
authors.initials: T
authors.lastname: Funahashi
authors.forename: Hitonobu
authors.initials: H
authors.lastname: Tomoike
authors.forename: Masafumi
authors.initials: M
authors.lastname: Kitakaze
mesh_terms: D017667:Adipocytes / Q000187:drug effects / Q000378:metabolism*; D052242:Adiponectin / Q000096:biosynthesis* / Q000097:blood / Q000235:genetics; D000328:Adult; D009320:Atrial Natriuretic Factor / Q000627:therapeutic use; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D005260:Female; D006333:Heart Failure / Q000097:blood / Q000188:drug therapy / Q000378:metabolism*; D006801:Humans; D008875:Middle Aged; D045283:Natriuretic Agents / Q000627:therapeutic use; D045265:Natriuretic Peptides / Q000235:genetics / Q000494:pharmacology*; D011446:Prospective Studies; D012333:RNA, Messenger / Q000378:metabolism; D011994:Recombinant Proteins / Q000494:pharmacology
chemical_list: D052242:Adiponectin; D045283:Natriuretic Agents; D045265:Natriuretic Peptides; D012333:RNA, Messenger; D011994:Recombinant Proteins; D009320:Atrial Natriuretic Factor