Regulation of DNA demethylation during maturation of CD4+ naive T cells by the conserved noncoding sequence 1.
Demethylation of transcriptional regulatory elements and gene coding regions is an important step in the epigenetic regulation of gene expression. Several noncoding conserved regions are required for the efficient transcription of cytokine genes. In this paper, we show that the deletion of one such sequence, conserved noncoding sequence 1 (CNS-1), interferes with the efficient demethylation of Th2 cytokine genes but has little effect on histone modifications in the area. Th2 cells derived from CD4 single-positive (SP) mature thymocytes exhibit more rapid demethylation of CNS-1 and Th2-specific cytokine genes and produce more Th2 cytokines than do Th2 cells derived from CD4-positive peripheral naive T cells. De-repression of the Th1 cytokine IFN-gamma was also detected in Th2-primed CD4 SP thymocytes but not in naive T cells. Our results indicate that susceptibility to demethylation determines the efficiency and kinetics of cytokine gene transcription. The extrathymic maturation step undergone by naive T cells suppresses robust and rapid cytokine expression, whereas mature CD4 SP thymocytes maintain a rapid and less-specific cytokine expression profile. Finally, we detected the methyl cytosine binding protein MBD2 at CNS-1 in mature thymocytes, suggesting that this protein may regulate the demethylation of this region.
Aoki
Kazuhisa
K
Cytokine Project, The Tokyo Metropolitan Institute of Medical Science, Tokyo, Japan.
Sato
Noriko
N
Yamaguchi
Atsumi
A
Kaminuma
Osamu
O
Hosozawa
Takumi
T
Miyatake
Shoichiro
S
D000818:Animals; D015496:CD4-Positive T-Lymphocytes / Q000166:cytology / Q000276:immunology* / Q000378:metabolism*; D002454:Cell Differentiation / Q000276:immunology*; D002478:Cells, Cultured; D017124:Conserved Sequence; D016207:Cytokines / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D019175:DNA Methylation; D017353:Gene Deletion; D006657:Histones / Q000378:metabolism; D007113:Immunity, Innate / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D022661:RNA, Untranslated / Q000235:genetics*; D013950:Thymus Gland / Q000276:immunology / Q000378:metabolism; D013997:Time Factors; D014158:Transcription, Genetic / Q000235:genetics
D016207:Cytokines; D006657:Histones; D022661:RNA, Untranslated