MicroPET, MicroSPECT, and NIR fluorescence imaging of biomolecules in vivo.
Molecular imaging is a newly merged multidisciplinary subject that requires contributions from biology, medical physics, and chemistry/radiochemistry. Integrin alpha(v)beta(3), a cell adhesion molecule, plays pivotal roles in regulating tumor angiogenesis and the growth of new blood vessels. In this chapter, we use the cell adhesion molecule integrin alpha(v)beta(3) as an example to demonstrate how one can synthesize appropriate arginine-glycine-aspartic acid (RGD) peptide-containing probes for visualizing and quantifying the receptor expression in vivo by means of microPET, microSPECT, and NIR fluorescence.
Li
Zi-Bo
ZB
Department of Radiology, Stanford University School of Medicine, 94305-5484, Stanford, CA, USA.
Chen
Xiaoyuan
X
D000818:Animals; D001943:Breast Neoplasms / Q000098:blood supply / Q000175:diagnosis / Q000378:metabolism; D045744:Cell Line, Tumor; D005260:Female; D005453:Fluorescence; D005462:Fluorine Radioisotopes; D005909:Glioblastoma / Q000098:blood supply / Q000175:diagnosis / Q000378:metabolism; D006801:Humans; D007259:Infrared Rays; D039302:Integrin alphaVbeta3 / Q000378:metabolism; D051379:Mice; D008819:Mice, Nude; D015336:Molecular Probe Techniques; D015335:Molecular Probes / Q000138:chemical synthesis / Q000378:metabolism*; D009389:Neovascularization, Pathologic; D009842:Oligopeptides / Q000138:chemical synthesis / Q000378:metabolism*; D049268:Positron-Emission Tomography / Q000379:methods*; D013667:Technetium; D015899:Tomography, Emission-Computed, Single-Photon / Q000379:methods*
D005462:Fluorine Radioisotopes; D039302:Integrin alphaVbeta3; D015335:Molecular Probes; D009842:Oligopeptides; D013667:Technetium; C047981:arginyl-glycyl-aspartic acid