Stabilizing the urinary activity of fructose-1,6-bisphosphatase with EDTA and mercaptoethanol.
OBJECTIVE
The study aim was to establish conditions for stabilization the activity of fructose-1,6-bisphosphatase (FBP-1) in stored urine.


METHODS
The FBP-1 was determined by the method Kepka et. al in a collected fraction of purified urine.


RESULTS
EDTA and mercaptoethanol stabilized FBP-1 activity in stored urine.


CONCLUSIONS
At optimal conditions urine may be stored up to 7 days at a temperature of 4 degrees C.
Kepka
Alina
A
Department of Biochemistry and Experimental Medicine, Children's Memorial Health Institute, Warsaw, Poland.
Szajda
Sławomir Dariusz
SD
Waszkiewicz
Napoleon
N
Zalewska
Anna
A
Płudowski
Paweł
P
Zwierz-Gugała
Dorota
D
Zalewska-Szajda
Beata
B
Borzym-Kluczyk
Małgorzata
M
Kryśkiewicz
Edyta
E
Zwierz
Krzysztof
K
D002614:Chelating Agents / Q000494:pharmacology; D002648:Child; D003404:Creatinine / Q000652:urine; D004492:Edetic Acid / Q000494:pharmacology*; D004795:Enzyme Stability / Q000187:drug effects; D005260:Female; D006597:Fructose-Bisphosphatase / Q000378:metabolism* / Q000652:urine*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008297:Male; D008623:Mercaptoethanol / Q000494:pharmacology*; D011309:Preservation, Biological / Q000379:methods; D013696:Temperature; D013997:Time Factors
D002614:Chelating Agents; D008623:Mercaptoethanol; D004492:Edetic Acid; D003404:Creatinine; D006597:Fructose-Bisphosphatase