Environmental applications of photoluminescence-based biosensors.
For monitoring and treatment of soil and water, environmental scientists and engineers require measurements of the concentration of chemical contaminants. Although laboratory-based methods relying on gas or liquid chromatography can yield very accurate measurements, they are also complex, time consuming, expensive, and require sample pretreatment. Furthermore, they are not readily adapted for in situ measurements.Sensors are devices that can provide continuous, in situ measurements, ideally without the addition of reagents. A biosensor incorporates a biological component coupled to a transducer, which translates the interaction between the analyte and the biocomponent into a signal that can be processed and reported. A wide range of transducers have been employed in biosensors, the most common of which are electrochemical and optical. In this contribution, we focus on photoluminescence-based biosensors of potential use in the applications described above.Following a review of photoluminescence and a discussion of the optoelectronic hardware part of these biosensor systems, we provide explanations and examples of optical biosensors for specific chemical groups: hydrocarbons and alcohols, halogenated organics, nitro-, phospho-, sulfo-, and other substituted organics, and metals and other inorganics. We also describe approaches that have been taken to describe chemical mixtures as a whole (biological oxygen demand and toxicity) since most environmental samples contain mixtures of unknown (and changing) composition. Finally, we end with some thoughts on future research directions that are necessary to achieve the full potential of environmental biosensors.
Reardon
Kenneth F
KF
Department of Chemical and Biological Engineering, Colorado State University, Fort Collins, CO, USA.
Zhong
Zhong
Z
Lear
Kevin L
KL
D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D002623:Chemistry Techniques, Analytical; D055615:Electrical Equipment and Supplies; D004777:Environment; D049449:Luminescence