Genotypic characterization of Pseudomonas aeruginosa isolated from human and animal sources in Egypt.
Two different techniques for the molecular typing of Pseudomonas aeruginosa were used to study the epidemiology of P. aeruginosa strains. Colonization with P. aeruginosa was studied by taking samples of human origin collected from urine, sputum samples of patients suffering from lung manifestations and patients exposed to third-degree burns. In addition, samples of animal origin were collected from mastitic milk and lung tissues of slaughtered calves and from the internal organs of diseased chickens. Typing of 18 isolates was performed by random amplified polymorphic DNA analysis and amplified fragment length polymorphism analysis. Computer-aided cluster analysis indicated that similar groups of related isolates were obtained by each method.
Osman
K M
KM
Department of Microbiology, Faculty of Veterinary Medicine, Cairo University, Cairo, Egypt. s_mougy@hotmail.com
Alabady
M S
MS
Ata
N S S M
NS
Ezzeldin
N A
NA
Aly
M A K
MA
D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000453:epidemiology / Q000382:microbiology; D002645:Chickens; D016000:Cluster Analysis; D004534:Egypt / Q000453:epidemiology; D014644:Genetic Variation; D005838:Genotype; D006801:Humans; D011201:Poultry Diseases / Q000453:epidemiology / Q000382:microbiology; D011552:Pseudomonas Infections / Q000453:epidemiology / Q000382:microbiology* / Q000662:veterinary; D011550:Pseudomonas aeruginosa / Q000235:genetics*; D019105:Random Amplified Polymorphic DNA Technique; D015047:Zoonoses