A depletion strategy for improved detection of human proteins from urine.
With rapidly growing interest in the urine proteome, methods for reducing sample complexity are becoming increasingly important. Depletion strategies for removal of high-abundance proteins from human urine have not been reported. A commercial kit designed for depletion of abundant proteins from plasma was evaluated for removing top proteins from urine of patients with proteinuria. The number of low-abundance proteins identified in urine after depletion increased nearly 2.5-fold.
Kushnir
Mark M
MM
ARUP Institute for Clinical and Experimental Pathology, 500 Chipeta Way, Salt Lake City, UT 84132, USA.
Mrozinski
Peter
P
Rockwood
Alan L
AL
Crockett
David K
DK
D001798:Blood Proteins / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification; D016022:Case-Control Studies; D002846:Chromatography, Affinity; D002853:Chromatography, Liquid; D015180:Electrophoresis, Gel, Two-Dimensional; D006801:Humans; D011507:Proteinuria; D020543:Proteome / Q000032:analysis*; D040901:Proteomics / Q000379:methods*; D011933:Reagent Kits, Diagnostic; D016622:Silver Staining; D053719:Tandem Mass Spectrometry; D014462:Ultrafiltration
D001798:Blood Proteins; D020543:Proteome; D011933:Reagent Kits, Diagnostic