title: [Effects of inhibition of ANG-2 expression in Ishikawa cell line].
abstract: OBJECTIVE
  To investigate the effect of RNA interference mediated angiopoietin-2 (ANG-2) gene silencing on human endometrial carcinoma cell line Ishikawa.
  
  
  METHODS
  Short hairpin RNA (shRNA) targeting ANG-2 gene was designed and transfected into Ishikawa cells with Lipofectamine 2000. The mRNA and protein expression level of ANG-2, proliferation, morphological changes, apoptosis, cell cycle and invasive ability of the cells after transfection were analyzed.
  
  
  RESULTS
  The shRNA targeting the human ANG-2 gene effectively decreased the expression of ANG-2 on both mRNA and protein level, the proliferation inhibition rate of the Ishikawa cells was 63.11%, cell apoptosis was induced, and the cell cycle was arrested in G1 phase. The apoptotic rate of the Ishikawa cells in the transfected group was significantly higher, and the invasive ability was decreased markedly, than that of control groups.
  
  
  CONCLUSIONS
  The shRNA targeting human ANG-2 gene could reduce ANG-2 expression, inhibit cellular growth and invasion in Ishikawa cells in vitro.
authors.affiliation: Department of Obstetrics and Gynecology, the Affiliated Drum Tower Hospital, Nanjing University School of Medicine, Nanjing, Jiangsu, 210008 PR China.
authors.forename: Huai-jun
authors.initials: HJ
authors.lastname: Zhou
authors.forename: Rong-rong
authors.initials: RR
authors.lastname: He
authors.forename: Bao-mei
authors.initials: BM
authors.lastname: Xia
authors.forename: Qing-ying
authors.initials: QY
authors.lastname: Xun
authors.forename: Jin-shun
authors.initials: JS
authors.lastname: Pan
authors.forename: Ya-li
authors.initials: YL
authors.lastname: Hu
mesh_terms: D042702:Angiopoietin-2 / Q000037:antagonists & inhibitors / Q000235:genetics*; D017209:Apoptosis / Q000187:drug effects / Q000235:genetics*; D002453:Cell Cycle / Q000187:drug effects / Q000235:genetics; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D016889:Endometrial Neoplasms / Q000235:genetics* / Q000473:pathology; D005260:Female; D015870:Gene Expression / Q000187:drug effects; D015972:Gene Expression Regulation, Neoplastic; D020868:Gene Silencing / Q000187:drug effects* / Q000502:physiology; D006801:Humans; D007202:Indicators and Reagents; D008055:Lipids / Q000494:pharmacology*; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics / Q000494:pharmacology*; D014162:Transfection; D014407:Tumor Cells, Cultured
chemical_list: D042702:Angiopoietin-2; D007202:Indicators and Reagents; D008055:Lipids; C086724:Lipofectamine; D034741:RNA, Small Interfering