title: Effect of the oligodendrocyte myelin glycoprotein (OMgp) on the expansion and neuronal differentiation of rat neural stem cells.
abstract: The oligodendrocyte myelin glycoprotein (OMgp) inhibits axon regeneration after injury in the adult mammalian central nervous system. However its function during brain development remains largely unknown. The present study aims to analyze a possible role for OMgp during neurogenesis. We showed that neural stem cells (NSC) extracted from the whole mesencephalon of rat embryos (E14) and cultured as free floating neurospheres expressed both OMgp and its receptor Nogo-R1. An over-expression of OMgp affected NSC expansion by reducing cell proliferation, but did not affect their differentiation into neurons. These findings indicate a new role for OMgp during brain development as a possible regulator of neurogenesis. Moreover, they suggest a possible implication for OMG gene in the etiology of neurofibromatosis type 1 forms characterized by a deletion of the NF1 gene locus containing OMG.
authors.affiliation: UMR Inserm U930, Université François Rabelais de Tours, Tours, France.
authors.forename: Isabelle
authors.initials: I
authors.lastname: Martin
authors.forename: Christian R
authors.initials: CR
authors.lastname: Andres
authors.forename: Sylviane
authors.initials: S
authors.lastname: Védrine
authors.forename: Refaat
authors.initials: R
authors.lastname: Tabagh
authors.forename: Caroline
authors.initials: C
authors.lastname: Michelle
authors.forename: Marie-Lise
authors.initials: ML
authors.lastname: Jourdan
authors.forename: Nathalie
authors.initials: N
authors.lastname: Heuze-Vourc'h
authors.forename: Philippe
authors.initials: P
authors.lastname: Corcia
authors.forename: Anne
authors.initials: A
authors.lastname: Duittoz
authors.forename: Patrick
authors.initials: P
authors.lastname: Vourc'h
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000502:physiology; D002454:Cell Differentiation / Q000502:physiology*; D049109:Cell Proliferation; D002478:Cells, Cultured; D005434:Flow Cytometry; D058851:GPI-Linked Proteins; D009185:Myelin Proteins; D018992:Myelin-Associated Glycoprotein / Q000378:metabolism*; D063308:Myelin-Oligodendrocyte Glycoprotein; D055495:Neurogenesis / Q000502:physiology*; D009474:Neurons / Q000166:cytology* / Q000378:metabolism; D000070816:Nogo Receptor 1; D051381:Rats; D017208:Rats, Wistar; D011956:Receptors, Cell Surface; D018000:Receptors, Peptide / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013234:Stem Cells / Q000166:cytology* / Q000378:metabolism
chemical_list: D058851:GPI-Linked Proteins; C571328:Mog protein, rat; D009185:Myelin Proteins; D018992:Myelin-Associated Glycoprotein; D063308:Myelin-Oligodendrocyte Glycoprotein; D000070816:Nogo Receptor 1; C560532:Omg protein, rat; D011956:Receptors, Cell Surface; D018000:Receptors, Peptide; C486581:Rtn4r protein, rat