title: Use of coherent control for selective two-photon fluorescence microscopy in live organisms.
abstract: We demonstrate selective fluorescence We demonstrate selective fluorescence excitation of specific molecular species in live organisms by using coherent control of two-photon excitation. We have acquired quasi-simultaneous images in live fluorescently-labeled Drosophila embryos by rapid switching between appropriate pulse shapes. Linear combinations of these images demonstrate that a high degree of fluorophore selectivity is attainable through phase-shaping. Broadband phase-shaped excitation opens up new possibilities for single-laser, multiplex, in-vivo fluorescence microscopy.
authors.forename: Jennifer P
authors.initials: JP
authors.lastname: Ogilvie
authors.forename: Delphine
authors.initials: D
authors.lastname: Débarre
authors.forename: Xavier
authors.initials: X
authors.lastname: Solinas
authors.forename: Jean-Louis
authors.initials: JL
authors.lastname: Martin
authors.forename: Emmanuel
authors.initials: E
authors.lastname: Beaurepaire
authors.forename: Manuel
authors.initials: M
authors.lastname: Joffre