title: Potentiometric monitoring DNA hybridization.
abstract: The usual procedure to monitor the ion exchange of small ions utilizes a potentiometer with a selective membrane as part of the working electrode. As the next step, we have applied polyaniline electrodes to the monitoring the activity macromolecular ions during DNA hybridization. Single-strand oligonucleotide (ssODN) probes were immobilized using a nucleophilic substitution reaction of the thiolated ssODN molecules with polyaniline. The anionic phosphate groups of the probe molecules also interacted with the cationic-doped polyaniline surface. Three useful findings were observed with the potentiometric experiments. First, the binding of the complimentary target molecules with the immobilized probes revealed a substantial potential change. Further, potential change was observed neither with the non-complimentary targets nor with the samples with a mutation in the sequence. The last two experiments were important for the future evaluation of the impact of medium and potential interfering compounds: anionic groups and hydrogen bonding groups in the non-complimentary samples did not cause any interactions.
authors.affiliation: Polymer Research Institute, Polytechnic Institute of New York University, NY, NY 11201, USA.
authors.forename: Y
authors.initials: Y
authors.lastname: Zhou
authors.forename: B
authors.initials: B
authors.lastname: Yu
authors.forename: A
authors.initials: A
authors.lastname: Guiseppi-Elie
authors.forename: V
authors.initials: V
authors.lastname: Sergeyev
authors.forename: K
authors.initials: K
authors.lastname: Levon
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation*; D004247:DNA / Q000032:analysis* / Q000737:chemistry* / Q000235:genetics; D004563:Electrochemistry / Q000295:instrumentation*; D004566:Electrodes; D004867:Equipment Design; D019544:Equipment Failure Analysis; D009693:Nucleic Acid Hybridization / Q000379:methods*
chemical_list: D004247:DNA