Stem cell factor and its soluble receptor (c-kit) in serum of asthmatic patients- correlation with disease severity.
BACKGROUND
SCF (stem cell factor) is a pleiotropic cytokine exerting its role at different stages of bone marrow development and affecting eosinophil activation, mast cells and basophil chemotaxis and survival. The aim of the study was to assess concentration of SCF and its soluble receptor c-kit (sc-kit) in peripheral blood of patients with asthma referring it to asthma severity and phenotype.


METHODS
The study involved 107 patients with bronchial asthma, well characterized with respect to severity and 21 healthy controls. Concentration of SCF and sc-kit in the patients serum were measured by ELISA method.


RESULTS
Mean serum SCF level in the group of asthmatics (n = 88) was significantly higher as compared to healthy controls (1010 pg/ml +/- 37 vs 799 +/- 33; p < 0,001). The level of SCF was higher in patients with severe asthma as compared to patients with non-severe asthma (1054 +/- 41 pg/ml vs 819 +/- 50; p < 0,01) and correlated with dose of inhaled glucocorticosteroids taken by the patients to achieve asthma control (R = 0,28; p < 0,01). The mean sc-kit serum level did not differ between asthmatic patients and healthy controls, however the level of sc-kit in non-severe asthmatics was significantly higher as compared to patients with severe asthma and healthy controls. In asthmatic patients (n = 63) the level of sc-kit correlated positively with FEV1% predicted value (R = 0,45; p < 0,001) and MEF25% predicted value (R = 0,33; p < 0,01). The level of sc-kit inversely correlated with the dose of inhaled glucocorticosteroids taken by the patients (R = -0,26; p < 0,01).


CONCLUSIONS
Serum levels of SCF and its soluble receptor c-kit seem to be reflect asthma severity suggesting a role for these molecules in asthmatic inflammation.
Makowska
Joanna S
JS
Department of Immunology, Rheumatology and Allergy, Medical University of Lodz, Lodz, Poland. czajka@csk.umed.lodz.pl
Cieslak
Malgorzata
M
Kowalski
Marek L
ML
D000328:Adult; D000368:Aged; D001249:Asthma / Q000276:immunology* / Q000378:metabolism* / Q000503:physiopathology; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005541:Forced Expiratory Volume; D006801:Humans; D008297:Male; D008875:Middle Aged; D011014:Pneumonia / Q000276:immunology / Q000378:metabolism / Q000503:physiopathology; D011237:Predictive Value of Tests; D019009:Proto-Oncogene Proteins c-kit / Q000097:blood*; D012720:Severity of Illness Index; D013147:Spirometry; D019089:Stem Cell Factor / Q000097:blood*; D055815:Young Adult
D019089:Stem Cell Factor; D019009:Proto-Oncogene Proteins c-kit