Synthesis of semitelechelic maleimide poly(PEGA) for protein conjugation by RAFT polymerization.
Maleimide end functionalized polymers for site-selective conjugation to free cysteines of proteins were synthesized using reversible addition-fragmentation chain transfer (RAFT) polymerization. A furan-protected maleimide chain transfer agent (CTA) was employed in the RAFT polymerization of poly(ethylene glycol) methyl ether acrylate (PEGA). Gel permeation chromatography (GPC) with laser light scattering detection indicated that 20,000 and 39,000 Da polyPEGA had been made with polydispersity indices of 1.25 and 1.36, respectively. The maleimide group on the polymer chain end was exposed by heating the poly(PEGA)s for 4 h. The deprotection efficiency was estimated to be 80 and 60% for poly(PEGA)(20 kDa) and poly(PEGA)(39 kDa), respectively. Maleimide-poly(PEGA)s were conjugated to V131C T4 lysozyme (T4L), and the resultant polymer-protein conjugates were characterized by size exclusion chromatography and gel electrophoresis.
Bays
Emmanuelle
E
Department of Chemistry and Biochemistry and the California NanoSystems Institute, University of California, Los Angeles, 607 Charles E. Young Drive East, Los Angeles, California 90095-1569, USA.
Tao
Lei
L
Chang
Chien-Wen
CW
Maynard
Heather D
HD
D002850:Chromatography, Gel; D003545:Cysteine; D004591:Electrophoresis, Polyacrylamide Gel; D008301:Maleimides / Q000737:chemistry*; D008970:Molecular Weight; D009113:Muramidase / Q000737:chemistry; D011092:Polyethylene Glycols / Q000138:chemical synthesis*; D058105:Polymerization; D011506:Proteins / Q000737:chemistry*
D008301:Maleimides; D011506:Proteins; C109511:bis(polyoxyethylene bis(amine)); C043592:maleimide; D011092:Polyethylene Glycols; D009113:Muramidase; D003545:Cysteine