title: Construction of EST database for comparative gene studies of Acanthamoeba.
abstract: The genus Acanthamoeba can cause severe infections such as granulomatous amebic encephalitis and amebic keratitis in humans. However, little genomic information of Acanthamoeba has been reported. Here, we constructed Acanthamoeba expressed sequence tags (EST) database (Acanthamoeba EST DB) derived from our 4 kinds of Acanthamoeba cDNA library. The Acanthamoeba EST DB contains 3,897 EST generated from amebae under various conditions of long term in vitro culture, mouse brain passage, or encystation, and downloaded data of Acanthamoeba from National Center for Biotechnology Information (NCBI) and Taxonomically Broad EST Database (TBestDB). The almost reported cDNA/genomic sequences of Acanthamoeba provide stand alone BLAST system with nucleotide (BLAST NT) and amino acid (BLAST AA) sequence database. In BLAST results, each gene links for the significant information including sequence data, gene orthology annotations, relevant references, and a BlastX result. This is the first attempt for construction of Acanthamoeba database with genes expressed in diverse conditions. These data were integrated into a database (http://www.amoeba.or.kr).
authors.affiliation: Department of Parasitology, Kyungpook National University School of Medicine, Daegu, Korea.
authors.forename: Eun-Kyung
authors.initials: EK
authors.lastname: Moon
authors.forename: Joung-Ok
authors.initials: JO
authors.lastname: Kim
authors.forename: Ying-Hua
authors.initials: YH
authors.lastname: Xuan
authors.forename: Young-Sun
authors.initials: YS
authors.lastname: Yun
authors.forename: Se Won
authors.initials: SW
authors.lastname: Kang
authors.forename: Yong Seok
authors.initials: YS
authors.lastname: Lee
authors.forename: Tae-In
authors.initials: TI
authors.lastname: Ahn
authors.forename: Yeon-Chul
authors.initials: YC
authors.lastname: Hong
authors.forename: Dong-Il
authors.initials: DI
authors.lastname: Chung
authors.forename: Hyun-Hee
authors.initials: HH
authors.lastname: Kong
mesh_terms: D000048:Acanthamoeba / Q000235:genetics*; D000818:Animals; D030541:Databases, Genetic; D020224:Expressed Sequence Tags