Correlated movement and bending of nucleic acid structures visualized by multicolor single-molecule spectroscopy.
At the end of the rainbow.... The structure and dynamics of three- and four- way DNA junctions are studied using triple alternating laser excitation. Steroid binding distorts the structure of three-way junctions with correlated bending of all branches towards a steeper pyramid (see picture). In four-way junctions, the dynamics of three distances are visualized simultaneously.
Person
Britta
B
Applied Physics-Biophysics, and Center for NanoScience, Ludwig-Maximilians Universität, München, Germany.
Stein
Ingo H
IH
Steinhauer
Christian
C
Vogelsang
Jan
J
Tinnefeld
Philip
P
D004247:DNA / Q000737:chemistry*; D045566:DNA, Cruciform / Q000737:chemistry*; D031541:Fluorescence Resonance Energy Transfer; D007834:Lasers; D009690:Nucleic Acid Conformation; D013050:Spectrometry, Fluorescence; D013256:Steroids / Q000737:chemistry
D045566:DNA, Cruciform; D013256:Steroids; D004247:DNA