[Protein profiles of multinodular hepatocellular carcinoma with multicentric occurrence or with intrahepatic metastasis].
OBJECTIVE
To analyze the protein expression profiles of multinodular hepatocellular carcinoma (HCC) with multicentric occurrence (MO) or with intrahepatic metastasis (IM).


METHODS
5 IM and 6 MO patients were divided into groups of IM1, IM2, MO1 and MO2 according to the size of node of HCC. Two dimensional gel electrophoresis (2-DE) and mass spectrum were used to analyze the protein expression profiles. Western blot was used to confirm the results obtained by mass spectrum.


RESULTS
2-DE of IM1, IM2, MO1 and MO2 indicated that 30 protein dots were differentially expressed in these tumors. By mass spectrum, 25 proteins were identified. Gene ontology classification indicated that these proteins are associated to cell movement, signal transduction, oxidoreduction, lipid metabolism, and amino acid metabolism.


CONCLUSIONS
The protein expression profiles of IM is different from that of MO, 2-DE and mass spectrum can be used to identify the molecular markers of IM and MO of HCC.
LI
Mei
M
Department of Clinical Laboratory, First Affiliated Hospital of Guangxi Medical University, Guangxi Medical University. Nanning 530021, China.
GUO
Kun
K
KANG
Xiao-nan
XN
SUN
Lu
L
SHU
Hong
H
LI
Ruo-lin
RL
XU
Ming-hui
MH
LIU
Yin-kun
YK
QIN
Xue
X
LI
Shan
S
D000328:Adult; D015153:Blotting, Western; D006528:Carcinoma, Hepatocellular / Q000378:metabolism* / Q000473:pathology; D015180:Electrophoresis, Gel, Two-Dimensional; D006801:Humans; D008113:Liver Neoplasms / Q000378:metabolism* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D009362:Neoplasm Metastasis; D009378:Neoplasms, Multiple Primary / Q000378:metabolism* / Q000473:pathology; D011379:Prognosis; D020543:Proteome / Q000378:metabolism*; D040901:Proteomics
D020543:Proteome