Dynamic light-activated control of local chemical concentration in a fluid.
This article reports a method for controlling the chemical concentration in a localized region of a fluid using optoelectrofluidic mechanisms. Optoelectrofluidic fluorescence microscopy (OFM), in which an optoelectrofluidic device is integrated into a conventional fluorescence microscope, allows both modulation and detection of local chemical concentration in an easy and simple way. Here, we present the first experimental investigation of the concentration change of polysaccharides, proteins, and fluorophores, due to the frequency-dependent ac electrokinetics and electrostatic interactions in an optoelectrofluidic device. The dynamic modulation of the local concentration of biomolecules such as dextran and serum albumin was demonstrated in a temporal and spatial manner. This OFM can be a useful tool for controlling the local chemical concentration in several chemical and biological applications.
Hwang
Hyundoo
H
Department of Bio and Brain Engineering, College of Life Science and Bioengineering, KAIST, 335 Gwanhangno, Yuseong-gu, Daejeon 305-701, Republic of Korea.
Park
Je-Kyun
JK
D000818:Animals; D002417:Cattle; D003911:Dextrans / Q000737:chemistry; D004560:Electricity; D016650:Fluorescein-5-isothiocyanate / Q000737:chemistry; D005452:Fluoresceins / Q000737:chemistry; D008027:Light; D008856:Microscopy, Fluorescence / Q000295:instrumentation* / Q000379:methods; D012710:Serum Albumin, Bovine / Q000737:chemistry; D055672:Static Electricity
D003911:Dextrans; D005452:Fluoresceins; D012710:Serum Albumin, Bovine; D016650:Fluorescein-5-isothiocyanate