Electrochemical assay of the nitrate and nitrite reductase activities of Rhizobium japonicum.
This work describes an electrochemical method for the determination of the nitrate and nitrite reductase activities of Rhizobium japonicum. The advantage of the method lies in the use of whole cells for the analysis and we earlier developed this protocol for the assay of NO. The results obtained are comparable to the spectrophotometric Griess assay. As the method is based on electrochemical reduction, the commonly interfering biological components like ascorbic acid, uric acid, dopamine, etc., will not interfere with the analysis. This method can be extended to the fabrication of biosensors for nitrate and nitrite using the same principle.
Salome
J Priscilla
JP
Central Electrochemical Research Institute, Electrodics and Electrocatalysis Division, Karaikudi 630006, Tamilnadu, India.
Amutha
R
R
Jagannathan
P
P
Josiah
J J M
JJ
Berchmans
Sheela
S
Yegnaraman
V
V
D015374:Biosensing Techniques / Q000295:instrumentation*; D004563:Electrochemistry / Q000295:instrumentation*; D004789:Enzyme Activation; D004867:Equipment Design; D019544:Equipment Failure Analysis; D050901:Nitrate Reductase / Q000032:analysis* / Q000737:chemistry*; D009572:Nitrite Reductases / Q000032:analysis* / Q000737:chemistry*; D012231:Rhizobium / Q000737:chemistry / Q000201:enzymology*
D009572:Nitrite Reductases; D050901:Nitrate Reductase