Composition of extracellular polymeric substances influences the autoaggregation capability of hydrogen-producing bacterium Ethanoligenens harbinense.
Non-spore-forming Ethanoligenens, a novel genus of hydrogen-producing bacteria, is endowed with great application potential in biohydrogen production due to acidophilic and autoaggregating growth. In order to elucidate the mechanism of autoaggregation of Ethanoligenens harbinense, extracellular polymeric substances (EPS) from YUAN-3 had been extracted and analyzed. The EPS was mainly produced during the exponential phase and with protein, carbohydrate and DNA as its main components, with yields of 21.0+/-0.8 mg/g-cell dry weight (CDW), 16.9+/-0.8 mg/g-CDW and 3.5+/-0.5 mg/g-CDW, respectively. Compared with the EPS composition of semi-autoaggregating hydrogen-producing bacteria W1 and non-autoaggregating hydrogen-producing bacteria B49, carbohydrate and protein played an important part in the autoaggregation of YUAN-3.
Ren
Nanqi
N
State Key Laboratory of Urban Water Resource and Environment, School of Municipal and Environmental Engineering, Harbin Institute of Technology, Harbin 150090, China.
Xie
Tianhui
T
Xing
Defeng
D
D001704:Biopolymers / Q000737:chemistry*; D002411:Cation Exchange Resins / Q000737:chemistry; D005110:Extracellular Space / Q000737:chemistry*; D006094:Gram-Positive Bacteria / Q000166:cytology* / Q000254:growth & development; D006859:Hydrogen / Q000378:metabolism*; D013997:Time Factors
D001704:Biopolymers; D002411:Cation Exchange Resins; D006859:Hydrogen