title: Hepatoprotective constituents from Cleome droserifolia.
abstract: The effect of ethanol extract from aerial parts of Cleome droserifolia was investigated against carbon tetrachloride induced liver injury. The hepatoprotective activity was evaluated through the quantification of biochemical parameters and confirmed using histopathology analysis. Efficient hepatoprotective effect was achieved by crude extract, fractions and some pure compounds. The phytochemical studies showed that the petroleum ether fraction afforded two known guaiane sesquiterpenes buchariol (1) and teucladiol (2) in addition to daucosterol (beta-sitosterol glucoside) (3). The CHCl(3) fraction afforded three known flavonoid derivatives; 5,3'-dihydroxy-3,6,7,4',5'-pentamethoxyflavone (4), 5'-hydroxy-3,6,7,3',4',5'-hexamethoxyflavone (5) and luteolin (6) and a known dolabellane diterpene (1R,2R,3E,7E,11R,12S)-2-O-acetyl-16-O-(3-hydroxy-3-methylglutaryl)-dolabella-3,7-dien-2,16,18-triol (7). The active parts of the MeOH fraction afforded the previously unreported 3'-methoxy-3,5,4'-trihydroxy flavone-7-neohesperidoside (8) and a known megastigmane norterpene; (6S,9R)-roseoside (9).
authors.affiliation: Department of Pharmacognosy, College of Pharmacy, King Saud University, P.O. Box 2457, Riyadh 11451, Kingdom of Saudi Arabia. mpharm101@hotmail.com
authors.forename: Maged Saad
authors.initials: MS
authors.lastname: Abdel-Kader
authors.forename: Saleh Ibrahim
authors.initials: SI
authors.lastname: Alqasoumi
authors.forename: Areej Mohammad
authors.initials: AM
authors.lastname: Al-Taweel
mesh_terms: D000410:Alanine Transaminase / Q000097:blood; D000469:Alkaline Phosphatase / Q000097:blood; D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood; D001663:Bilirubin / Q000378:metabolism; D002240:Carbohydrate Sequence; D056486:Chemical and Drug Induced Liver Injury / Q000473:pathology / Q000517:prevention & control*; D002725:Chloroform / Q000737:chemistry; D031485:Cleome / Q000737:chemistry*; D000431:Ethanol; D008099:Liver / Q000473:pathology; D008111:Liver Function Tests; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D000432:Methanol / Q000737:chemistry; D008969:Molecular Sequence Data; D010936:Plant Extracts / Q000737:chemistry; D020011:Protective Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D012997:Solvents; D013056:Spectrophotometry, Ultraviolet
chemical_list: D010936:Plant Extracts; D020011:Protective Agents; D012997:Solvents; D000431:Ethanol; D002725:Chloroform; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D000469:Alkaline Phosphatase; D001663:Bilirubin; D000432:Methanol