Bactrocerin-1: a novel inducible antimicrobial peptide from pupae of oriental fruit fly Bactrocera dorsalis Hendel.
A novel antimicrobial peptide, Bactrocerin-1, was purified and characterized from an immunized dipteran insect, Bactrocera dorsalis. Bactrocerin-1 has 20 amino acid residues with a mass of 2,325.95 Da. The amino acid sequence of Bactrocerin-1 showed very high similarity to the active fragment (46V-65S-NH(2)) of Coleoptericin A. The composition of amino acid residues revealed that Bactrocerin-1 is a hydrophobic, positively charged, and Lys/Ile/Gly-rich peptide. Minimal growth inhibition concentration (MIC) measurements for synthesized Bactrocerin-1 showed a very broad spectrum of anti-microbial activity against Gram-positive bacteria, Gram-negative bacteria, and fungi. Bactrocerin-1 did not show hemolytic activity toward mouse red blood cells even at a concentration of 50 microM. Analysis of the Helical-wheel projection and the CD spectrum suggested that Bactrocerin-1 contains the amphipathic alpha-helix.
Dang
Xiang-Li
XL
Zhejiang Institute of Subtropical Crops, Wenzhou, P.R. China.
Tian
Jin-Huan
JH
Yang
Wan-Ying
WY
Wang
Wen-Xian
WX
Ishibashi
Jun
J
Asaoka
Ai
A
Yi
Hui-Yu
HY
Li
Yi-Feng
YF
Cao
Yang
Y
Yamakawa
Minoru
M
Wen
Shuo-Yang
SY
D000595:Amino Acid Sequence; D000818:Animals; D000890:Anti-Infective Agents / Q000494:pharmacology*; D002942:Circular Dichroism; D004591:Electrophoresis, Polyacrylamide Gel; D005658:Fungi / Q000187:drug effects; D006090:Gram-Negative Bacteria / Q000187:drug effects; D006094:Gram-Positive Bacteria / Q000187:drug effects; D019476:Insect Proteins / Q000302:isolation & purification / Q000494:pharmacology*; D051379:Mice; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D017386:Sequence Homology, Amino Acid; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D033621:Tephritidae / Q000737:chemistry* / Q000254:growth & development
D000890:Anti-Infective Agents; D019476:Insect Proteins; C543939:bactrocerin-1, Bactrocera dorsalis