Evaluation of cryopreserved donor skin viability: the experience of the regional tissue bank of Verona.
BACKGROUND
Allogeneic human skin removed from cadaveric donors is the covering of choice for deep burns, since it accelerates the re-epithelialisation of autologous skin. In this study we evaluated the cellular viability of cryopreserved skin at the regional tissue bank of Verona (Italy).


METHODS
From 1st June 2007 to 30th September 2007, tests of cutaneous cell viability were carried out on 21 consecutive skin donors using the MTT (tetrazolium salt) method on samples prior to freezing and on thawed samples after a period of cryopreservation.


RESULTS
The mean percentage viability was 45.1% (+/-20.1%), which is similar to results obtained in other tissue banks. It was noted that viability decreased with increasing age of the donor.


CONCLUSIONS
The results of the evaluation of cutaneous cell viability document the validity of the skin cryopreservation procedure in use at the tissue bank in Verona.
Franchini
Massimo
M
Servizio di Immunoematologia e Medicina Trasfusionale, Dipartimento di Patologia e Medicina di Laboratorio, Azienda Ospedaliero - Universitaria di Parma, Parma, Italy. mfranchini@ao.pr.it
Zanini
Daniela
D
Bosinelli
Adelia
A
Fiorini
Silvia
S
Rizzi
Sandra
S
D'Aloja
Chiara
C
Vassanelli
Aurora
A
Gandini
Giorgio
G
Aprili
Giuseppe
G
D000465:Algorithms; D002056:Burns / Q000601:surgery*; D002102:Cadaver; D002470:Cell Survival; D015925:Cryopreservation / Q000379:methods; D006801:Humans; D007202:Indicators and Reagents / Q000493:pharmacokinetics; D007558:Italy; D012189:Retrospective Studies; D012867:Skin; D016038:Skin Transplantation; D013778:Tetrazolium Salts; D014015:Tissue Banks; D014021:Tissue Preservation / Q000379:methods; D014184:Transplantation, Homologous
D007202:Indicators and Reagents; D013778:Tetrazolium Salts