Carrier PNA for shRNA delivery into cells.
A peptide nucleic acid (PNA)-cell-penetrating peptide (CPP) conjugate (carrier PNA) was used as 'bridge-builder' to connect a CPP with an shRNA. The carrier PNA successfully formed a hybrid with an shRNA bearing complementary dangling bases and the shRNA was introduced into cells by the carrier PNA, and RNAi was induced by the shRNA.
Kitamatsu
Mizuki
M
Department of Bioscience and Biotechnology, Faculty of Engineering, Okayama University, 3-1-1 Tsushimanaka, Okayama 700-0082, Japan. kitamatu@cc.okayama-u.ac.jp
Kubo
Takanori
T
Matsuzaki
Rino
R
Endoh
Tamaki
T
Ohtsuki
Takashi
T
Sisido
Masahiko
M
D000818:Animals; D001483:Base Sequence; D016466:CHO Cells; D006224:Cricetinae; D003412:Cricetulus; D020135:Peptide Nucleic Acids / Q000737:chemistry* / Q000378:metabolism; D034622:RNA Interference; D012330:RNA, Double-Stranded / Q000008:administration & dosage* / Q000737:chemistry / Q000378:metabolism; D044366:Transition Temperature
D020135:Peptide Nucleic Acids; D012330:RNA, Double-Stranded