Direct detection of genomic DNA with fluidic force discrimination assays.
Herein, we describe the direct detection of genomic DNA using fluidic force discrimination (FFD) assays. Starting with extracted bacterial DNA, samples are fragmented by restriction enzymes or sonication, then thermocycled in the presence of blocking and labeling sequences, and finally detected with microbead-based FFD assays. Both strain and species identification of extracted Bacillus DNA have been demonstrated in <30 min, without amplification (e.g., PCR). Femtomolar assays can be achieved with this rapid and simple procedure.
Mulvaney
S P
SP
Naval Research Laboratory, Washington, DC 20375-5342, USA. shawn.mulvaney@nrl.navy.mil
Ibe
C N
CN
Tamanaha
C R
CR
Whitman
L J
LJ
D001408:Bacillus anthracis / Q000235:genetics; D001413:Bacillus thuringiensis / Q000235:genetics; D004269:DNA, Bacterial / Q000032:analysis* / Q000235:genetics; D016680:Genome, Bacterial; D046210:Microfluidic Analytical Techniques / Q000379:methods*
D004269:DNA, Bacterial