Real-time and label-free detection of chloramphenicol residues with specific molecular interaction.
Molecular measurements with specific or non-specific molecular interaction are usually reported by using different methods. This paper shows a method for small-molecular label-free measurements in real time with specific and non-specific molecular interaction based on surface plasmon resonance. The quantitative measurement of glucose with non-specific molecular interaction was performed simply to validate our self-built angle-dependent surface plasmon resonance system, which showed a good sensitivity and stability, such as that of Fourier transform infrared spectrometer. As application, we present the measurement of chloramphenicol residues with specific molecular interaction employing an indirect competitive immunoreaction by using the same surface plasmon resonance system with a limit of detection about 0.5 ng mL(-1), which shows a potential application for food safety.
Dong
Z
Z
Department of Biomedical Engineering, the School of Medicine, Tsinghua University, Beijing 100084, China.
Huang
G
G
Xu
S
S
Deng
C
C
Zhu
J
J
Chen
S
S
Yang
X
X
Zhao
S
S
D002701:Chloramphenicol / Q000032:analysis*; D007118:Immunoassay / Q000379:methods; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D020349:Surface Plasmon Resonance / Q000379:methods*
D002701:Chloramphenicol