title: Large gradient high magnetic field affects the association of MACF1 with actin and microtubule cytoskeleton.
abstract: The intense inhomogeneous magnetic fields acting on the diamagnetic materials naturally present in cells can generate strong magnetic forces. We have developed a superconducting magnet platform with large gradient high magnetic field (LG-HMF), which can produce three magnetic force fields of -1360, 0, and 1312 T(2)/m, and three corresponding apparent gravity levels, namely 0, 1, and 2-g for diamagnetic materials. In this study, the effects of different magnetic force fields on osteoblast-like cells (MG-63 and MC3T3-E1) viability, microtubule actin crosslinking factor 1 (MACF1) expression and its association with cytoskeleton were investigated. Results showed that cell viability increased to different degrees after exposure to 0 or 1-g conditions for 24 h, but it decreased by about 30% under 2-g conditions compared with control conditions. An increase in MACF1 expression at the RNA or protein level was observed in osteoblast-like cells under the magnetic force field of -1360 T(2)/m (0-g) relative to 1312 T(2)/m (2-g). Under control conditions, anti-MACF1 staining was scattered in the cytoplasm and partially colocalized with actin filaments (AFs) or microtubules (MTs) in the majority of osteoblast-like cells. Under 0-g conditions, MACF1 labeling was concentrated at perinuclear region and colocalization was not apparent. The patterns of anti-MACF1 labeling on MTs varied with MTs' changing under LG-HMF environment. In conclusion, LG-HMF affects osteoblast-like cell viability, MACF1 distribution, expression, and its association with cytoskeleton to some extent.
authors.affiliation: Key Laboratory for Space Biosciences & Biotechnology, Institute of Special Environmental Biophysics, Faculty of Life Sciences, Northwestern Polytechnical University, Xi'an, Shaan Xi Province, PR China.
authors.forename: Ai-Rong
authors.initials: AR
authors.lastname: Qian
authors.forename: Li-Fang
authors.initials: LF
authors.lastname: Hu
authors.forename: Xiang
authors.initials: X
authors.lastname: Gao
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.forename: Sheng-Meng
authors.initials: SM
authors.lastname: Di
authors.forename: Zong-Cheng
authors.initials: ZC
authors.lastname: Tian
authors.forename: Peng-Fei
authors.initials: PF
authors.lastname: Yang
authors.forename: Da-Chuan
authors.initials: DC
authors.lastname: Yin
authors.forename: Yuan-Yuan
authors.initials: YY
authors.lastname: Weng
authors.forename: Peng
authors.initials: P
authors.lastname: Shang
mesh_terms: D000199:Actins / Q000378:metabolism*; D000818:Animals; D002460:Cell Line; D003599:Cytoskeleton / Q000378:metabolism* / Q000528:radiation effects*; D004307:Dose-Response Relationship, Radiation; D004574:Electromagnetic Fields; D051379:Mice; D008840:Microfilament Proteins / Q000378:metabolism*; D008870:Microtubules / Q000378:metabolism* / Q000528:radiation effects*; D010006:Osteoblasts / Q000378:metabolism* / Q000528:radiation effects; D011829:Radiation Dosage
chemical_list: D000199:Actins; C490632:Macf1 protein, mouse; D008840:Microfilament Proteins