Protein assembly onto cationic supported bilayers.
Cationic supported bilayers on latex are useful to isolate and immobilize oppositely charged proteins as a monomolecular layer over a range of low protein concentrations and particle number densities. Cholera toxin (CT) from Vibrio cholerae, an 87 kDa AB5 hexameric protein and bovine serum albumin (BSA) self-assembled on dioctadecyldimethylammonium bromide (DODAB) supported bilayers with high affinity yielding highly organized and monodisperse particulates at 5 x 10(9) particles/mL, over a range of low protein concentrations (0-0.025 mg/mL BSA or CT). Protein association onto the bilayer-covered polystyrene sulfate (PSS) was determined from adsorption isotherms, dynamic light scattering for size distributions and zeta-potential analysis revealing a monomolecular, thin and highly organized protein layer surrounding each particle with potential for biospecific recognition such as antigen-antibody, receptor-ligand, hybridization of oligonucleotide sequences, all of them important in immunodiagnosis, selective biomolecular chromatographic separations, microarrays design and others.
Lincopan
Nilton
N
Departamento de Bioquímica, Instituto de Química, Universidade de São Paulo, São Paulo,Caixa Postal 26077, São Paulo-SF, Brazil.
Carmona-Ribeiro
Ana Maria
AM
D000327:Adsorption; D002412:Cations; D002772:Cholera Toxin / Q000737:chemistry; D008051:Lipid Bilayers; D018716:Molecular Mimicry; D011506:Proteins / Q000737:chemistry*; D000644:Quaternary Ammonium Compounds / Q000737:chemistry; D012710:Serum Albumin, Bovine / Q000737:chemistry; D013816:Thermodynamics
D002412:Cations; D008051:Lipid Bilayers; D011506:Proteins; D000644:Quaternary Ammonium Compounds; C015831:dimethyldioctadecylammonium; D012710:Serum Albumin, Bovine; D002772:Cholera Toxin