title: Lebectin increases N-cadherin-mediated adhesion through PI3K/AKT pathway.
abstract: Cell adhesion molecules, including cadherins and integrins, play an essential role during tumor progression and represent potential targets for the development of new therapeutic agents. We previously showed that lebectin, a C-type lectin protein (CLP) issued from Macrovipera lebectina snake venom, inhibits integrin-mediated migration of IGR39 melanoma cells. Here we assessed whether lebectin modulates cell-cell adhesion. We demonstrated that lebectin promotes N-cadherin/catenin complex reorganization at cell-cell contacts, inducing a strengthening of intercellular adhesion. This reorganization is associated to phosphorylation of beta-catenin on tyrosine 142 residue. Interestingly, lebectin acts on N-cadherin-mediated cell-cell contacts through PI3K/Akt pathway. This effect could contribute to the blockage of tumor cell migration previously observed.
authors.affiliation: Institut Pasteur de Tunis, Tunis, Tunisia.
authors.forename: Sameh
authors.initials: S
authors.lastname: Sarray
authors.forename: Carole
authors.initials: C
authors.lastname: Siret
authors.forename: Maxime
authors.initials: M
authors.lastname: Lehmann
authors.forename: Naziha
authors.initials: N
authors.lastname: Marrakchi
authors.forename: José
authors.initials: J
authors.lastname: Luis
authors.forename: Mohamed
authors.initials: M
authors.lastname: El Ayeb
authors.forename: Frédéric
authors.initials: F
authors.lastname: André
mesh_terms: D015153:Blotting, Western; D015820:Cadherins / Q000187:drug effects* / Q000378:metabolism; D002448:Cell Adhesion / Q000187:drug effects; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects; D006801:Humans; D007150:Immunohistochemistry; D047468:Immunoprecipitation; D037181:Lectins, C-Type; D019869:Phosphatidylinositol 3-Kinases / Q000187:drug effects* / Q000378:metabolism; D051057:Proto-Oncogene Proteins c-akt / Q000187:drug effects* / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects* / Q000502:physiology; D014757:Viper Venoms / Q000494:pharmacology*
chemical_list: D015820:Cadherins; D037181:Lectins, C-Type; D014757:Viper Venoms; C519594:lebectin protein, Macrovipera lebetina; D019869:Phosphatidylinositol 3-Kinases; D051057:Proto-Oncogene Proteins c-akt