Blue Dry Western: simple, economic, informative, and fast way of immunodetection.
The analysis by electrophoresis followed by transfer to membranes and immunodetection (Western blot) is probably the most popular technique in protein study. Accordingly, it is a time- and money-consuming procedure. Here a protocol is described where immunodetection can be accomplished in 30 min. This approach also allows permanent staining of proteins by Coomassie Blue R on the membrane before immune staining with clear background and high sensitivity.
Naryzhny
Stanislav N
SN
Tumor Biology Group, Northeastern Ontario Regional Cancer Program at Sudbury Regional Hospital, Sudbury, Ont. P3E5J1, Canada. snaryzhny@hrsrh.on.ca
D015153:Blotting, Western / Q000191:economics / Q000379:methods*; D002460:Cell Line; D004396:Coloring Agents / Q000032:analysis*; D006801:Humans; D012394:Rosaniline Dyes / Q000032:analysis*; D013997:Time Factors
D004396:Coloring Agents; D012394:Rosaniline Dyes; C048139:Coomassie blue