title: Production of plant-specific tyramine derivatives by dual expression of tyramine N-hydroxycinnamoyltransferase and 4-coumarate:coenzyme A ligase in Escherichia coli.
abstract: Plant-specific bioactive compounds including feruloyltyramine (FT), 4-coumaroyltyramine (CT), and caffeoyltyramine (CaT) were simultaneously produced in Escherichia coli by heterologous expression of two biosynthetic genes encoding 4-coumarate:coenzyme A ligase and tyramine N-hydroxycinnamoyltransferase (THT) cloned from Arabidopsis thaliana and pepper, respectively. Simultaneous supplementation of substrates to the recombinant E. coli resulted in the secretion of multiple tyramine derivatives into the medium at high yield: CT (189 mg l(-1)), FT (135 mg l(-1)), CaT (40 mg l(-1)). In addition, the recombinant E. coli also produced, albeit at low concentration, a range of dopamine derivatives such as feruloyldopamine due to THT's ability to accept dopamine as a substrate.
authors.affiliation: Department of Biotechnology, Interdisciplinary Program for Bioenergy and Biomaterials of Graduate School, Biotechnology Research Institute, Chonnam National University, Gwangju 500-757, Republic of Korea.
authors.forename: Kiyoon
authors.initials: K
authors.lastname: Kang
authors.forename: Munyoung
authors.initials: M
authors.lastname: Park
authors.forename: Sangkyu
authors.initials: S
authors.lastname: Park
authors.forename: Young Soon
authors.initials: YS
authors.lastname: Kim
authors.forename: Sungbeom
authors.initials: S
authors.lastname: Lee
authors.forename: Seong-Gene
authors.initials: SG
authors.lastname: Lee
authors.forename: Kyoungwhan
authors.initials: K
authors.lastname: Back
mesh_terms: D000217:Acyltransferases / Q000235:genetics* / Q000378:metabolism*; D017360:Arabidopsis / Q000201:enzymology* / Q000235:genetics; D002109:Caffeic Acids / Q000378:metabolism; D003066:Coenzyme A Ligases / Q000235:genetics* / Q000378:metabolism*; D003373:Coumaric Acids / Q000378:metabolism; D003470:Culture Media / Q000737:chemistry; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D015870:Gene Expression; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D014439:Tyramine / Q000031:analogs & derivatives* / Q000378:metabolism*
chemical_list: D002109:Caffeic Acids; D003373:Coumaric Acids; D003470:Culture Media; C001411:N-(4-hydroxy-beta-phenethyl)-4-hydroxycinnamide; D011994:Recombinant Proteins; C481438:N-caffeoyltyramine; C074004:feruloyltyramine; D000217:Acyltransferases; C108168:hydroxycinnamoyl-CoA-tyramine N-(hydroxycinnamoyl)transferase; D003066:Coenzyme A Ligases; C019943:4-coumarate-CoA ligase; D014439:Tyramine