Coaxial electrohydrodynamic spraying: a novel one-step technique to prepare oligodeoxynucleotide encapsulated lipoplex nanoparticles.
This study investigates coaxial electrohydrodynamic spraying (electrospray for short) as a novel, rapid, real time and single-step method to produce oligodeoxynucleotide (ODN) encapsulated lipoplex nanoparticles for either intravenous injection or, potentially, pulmonary delivery. Using a coaxial needle setup, we produced G3139 (oblimerson sodium, or Genasense) encapsulated lipoplex nanoparticles, and investigated the effects of production parameters on nanoparticle size and structure. Careful control of production parameters yielded lipoplex nanoparticles 190 +/- 39 nm in diameter with unilamellar structure and 90 +/- 6% encapsulation efficiency of G3139. Both nontargeted and transferrin-targeted G3139 lipoplex nanoparticles were efficiently delivered to K562 cells and downregulated the bcl-2 protein expression by 34 +/- 6% and 57 +/- 3% respectively.
Wu
Yun
Y
William G. Lowrie Department of Chemical and Biomolecular Engineering, The Ohio State University, 140 West 19th Avenue, Columbus, Ohio 43210, USA.
Yu
Bo
B
Jackson
Andrew
A
Zha
Weibin
W
Lee
L James
LJ
Wyslouzil
Barbara E
BE
D001483:Base Sequence; D020285:Cryoelectron Microscopy; D015536:Down-Regulation / Q000187:drug effects; D016503:Drug Delivery Systems; D055664:Electrochemical Techniques / Q000379:methods*; D019254:Genes, bcl-2; D006801:Humans; D020014:K562 Cells; D008051:Lipid Bilayers / Q000737:chemistry* / Q000493:pharmacokinetics; D008564:Membrane Potentials; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D036103:Nanotechnology / Q000379:methods; D033363:Neutron Diffraction; D009838:Oligodeoxyribonucleotides / Q000008:administration & dosage* / Q000235:genetics / Q000493:pharmacokinetics; D010316:Particle Size; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D053838:Scattering, Small Angle; D014168:Transferrin / Q000378:metabolism
D008051:Lipid Bilayers; D009838:Oligodeoxyribonucleotides; D019253:Proto-Oncogene Proteins c-bcl-2; D014168:Transferrin