PDCD4 inhibits the malignant phenotype of ovarian cancer cells.
Programmed cell death 4 (PDCD4) is a newly identified tumor suppressor that can inhibit activator protein (AP)-1 activation and protein translation. Our previous studies indicate that lost or reduced PDCD4 expression is associated with the progression of ovarian carcinoma. However, direct evidence that PDCD4 inhibits malignant phenotype of human cancer cells is limited. In the present study, we found that PDCD4 expression in ovarian cancer cell lines (SKOV3, 3AO, and CAOV3) inhibited significantly their proliferation and cell cycle progression, and induced apoptosis. More importantly, up-regulation of PDCD4 expression decreased the colony-forming capacity of ovarian cancer cells in vitro and tumorigenic capacity in mice. These results demonstrate that PDCD4 can suppress the malignant phenotype of ovarian cancer cells, and may represent a novel therapeutic target for the treatment of ovarian cancer.
Wei
Zeng-Tao
ZT
Institute of Immunology, Chinese Ministry of Education and Chinese Ministry of Health, Qilu Hospital, Jinan, China.
Zhang
Xia
X
Wang
Xiao-Yan
XY
Gao
Fei
F
Zhou
Cheng-Jun
CJ
Zhu
Fa-Liang
FL
Wang
Qun
Q
Gao
Qi
Q
Ma
Chun-Hong
CH
Sun
Wen-Sheng
WS
Fu
Qing-Zhao
QZ
Chen
You-Hai H
YH
Zhang
Li-Ning
LN
D000818:Animals; D017209:Apoptosis / Q000235:genetics; D051017:Apoptosis Regulatory Proteins / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D002277:Carcinoma / Q000235:genetics / Q000473:pathology*; D002453:Cell Cycle; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D016147:Genes, Tumor Suppressor; D006801:Humans; D007150:Immunohistochemistry; D051379:Mice; D008819:Mice, Nude; D010051:Ovarian Neoplasms / Q000235:genetics / Q000473:pathology*; D016601:RNA-Binding Proteins / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D011897:Random Allocation; D018808:Transcription Factor AP-1 / Q000037:antagonists & inhibitors; D014162:Transfection; D047368:Tumor Burden; D023041:Xenograft Model Antitumor Assays
D051017:Apoptosis Regulatory Proteins; C114487:PDCD4 protein, human; D016601:RNA-Binding Proteins; D018808:Transcription Factor AP-1