Activities of antioxidant enzymes in three bacteria exposed to bensulfuron-methyl.
Oxidative stress enzymes, superoxide dismutase (SOD), catalase (CAT), and ATPase, from two Gram-positive bacteria and one Gram-negative bacterium, respectively, were tested for response to the oxidative stress caused by bensulfuron-methyl (BSM). Native PAGE was used to detect the SOD isoenzyme profiles of these bacteria. All three bacteria possessed a basal level of SOD, CAT, and ATPase activity prior to being exposed to BSM. Enzyme activities changed in a BSM-concentration-dependent manner after exposure to BSM for 24 h. Activity of all the enzymes was increased and reached the first activity peak after being exposed to BSM at 1 or 1.5 h, respectively, then a decline occurred, and after that another simulation appeared at 9h or 14 h. Only one and three detectable SOD isoenzyme bands were observed in Gram-positive strains and the Gram-negative strain, respectively. BSM could bring short-term ecotoxicity to three bacteria, but the effect of BSM was not lethal.
Lin
Xiaoyan
X
Department of Microbiology, College of Life Sciences, Zhejiang University, Hangzhou, People's Republic of China.
Xu
Xiaohong
X
Yang
Chinghong
C
Zhao
Yuhua
Y
Feng
Zhihong
Z
Dong
Yangyang
Y
D000251:Adenosine Triphosphatases / Q000378:metabolism; D000975:Antioxidants / Q000378:metabolism*; D001410:Bacillus megaterium / Q000187:drug effects / Q000201:enzymology; D001412:Bacillus subtilis / Q000187:drug effects / Q000201:enzymology; D002374:Catalase / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D004784:Environmental Monitoring; D004785:Environmental Pollutants / Q000633:toxicity*; D048168:Escherichia coli K12 / Q000187:drug effects / Q000201:enzymology; D006090:Gram-Negative Bacteria / Q000187:drug effects* / Q000201:enzymology*; D006094:Gram-Positive Bacteria / Q000187:drug effects* / Q000201:enzymology*; D007527:Isoenzymes; D018384:Oxidative Stress / Q000187:drug effects; D017382:Reactive Oxygen Species / Q000378:metabolism; D013453:Sulfonylurea Compounds / Q000633:toxicity*; D013482:Superoxide Dismutase / Q000378:metabolism; D018675:Toxicity Tests
D000975:Antioxidants; D004785:Environmental Pollutants; D007527:Isoenzymes; D017382:Reactive Oxygen Species; D013453:Sulfonylurea Compounds; C071475:methyl bensulfuron; D002374:Catalase; D013482:Superoxide Dismutase; D000251:Adenosine Triphosphatases