title: In vitro toxicity of MTA compared with other primary teeth pulpotomy agents.
abstract: OBJECTIVE
  The main goal of this work is to compare the In vitro toxicity of MTA with other primary teeth pulpotomy agents.
  
  
  METHODS
  The In vitro toxicity of MTA, calcium hydroxide, ferric sulphate solution, diluted formocresol and Buckley's formocresol were tested using MTT and Neutral Red Uptake cell viability assays. The results for MTA were compared to those obtained for the other substances using ANOVA and Tukey statistical tests (p < 0.05).
  
  
  RESULTS
  MTA had the lower in vitro toxicity and Buckley's formocresol, the higher, with statically significant difference.
  
  
  CONCLUSIONS
  Among the primary teeth pulpotomy agents tested, MTA showed the lower In vitro toxicity, standing as the most promising substitute to formocresol.
authors.affiliation: Pediatric Dentistry, Universidade Federal do Paraná, Curitiba, Brazil. jvmenezes@gmail.com
authors.forename: José Vitor Nogara Borges
authors.initials: JV
authors.lastname: de Menezes
authors.forename: Esther Rieko
authors.initials: ER
authors.lastname: Takamori
authors.forename: Maria Francisca Thereza Borro
authors.initials: MF
authors.lastname: Bijella
authors.forename: José Mauro
authors.initials: JM
authors.lastname: Granjeiro
mesh_terms: D016475:3T3 Cells; D017607:Aluminum Compounds / Q000633:toxicity*; D000818:Animals; D017610:Calcium Compounds / Q000633:toxicity*; D002126:Calcium Hydroxide / Q000633:toxicity; D002462:Cell Membrane / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D004396:Coloring Agents; D004338:Drug Combinations; D005290:Ferric Compounds / Q000633:toxicity; D005566:Formocresols / Q000633:toxicity; D007928:Lethal Dose 50; D051379:Mice; D008928:Mitochondria / Q000187:drug effects; D009499:Neutral Red; D010087:Oxides / Q000633:toxicity*; D011672:Pulpotomy / Q000009:adverse effects; D012387:Root Canal Filling Materials / Q000633:toxicity*; D017640:Silicates / Q000633:toxicity*; D013778:Tetrazolium Salts; D013844:Thiazoles
chemical_list: D017607:Aluminum Compounds; D017610:Calcium Compounds; D004396:Coloring Agents; D004338:Drug Combinations; D005290:Ferric Compounds; D005566:Formocresols; D010087:Oxides; D012387:Root Canal Filling Materials; D017640:Silicates; D013778:Tetrazolium Salts; D013844:Thiazoles; C086631:mineral trioxide aggregate; D009499:Neutral Red; C031025:formocresol; C024823:ferric sulfate; C022616:thiazolyl blue; D002126:Calcium Hydroxide