Purification of rutin and nicotiflorin from the flowers of Edgeworthia chrysantha Lindl. by high-speed counter-current chromatography.
An ethanol extract of air-dried flowers of Edgeworthia chrysantha Lindl. was partitioned between water and petroleum, ethyl acetate, and n-butanol. The n-butanol extraction was initially purified by silica gel column chromatography to give a partially purified sample. The bioactive compound rutin, along with nicotiflorin, were successfully separated from the partially purified sample by high-speed counter-current chromatography. The two compounds were isolated from the plant of Edgeworthia genus for the first time. The two-phase solvent system used was composed of ethyl acetate-n-butanol-water at an optimized ratio of 4:1:5 (v/v/v). High-speed counter-current chromatography yielded, from 108 mg of the partially purified extract, 53 mg rutin and 32 mg nicotiflorin with 92.5% and 92.2% recovery, with each at over 96.5% purity by high-performance liquid chromatography analysis. Their structures were identified by 1H NMR and 13C NMR.
Tong
Shengqiang
S
College of Pharmaceutical Science, Zhejiang University of Technology, Hangzhou, 310032, China.
Yan
Jizhong
J
Chen
Gang
G
Lou
Jianzhong
J
D002851:Chromatography, High Pressure Liquid; D003377:Countercurrent Distribution / Q000379:methods*; D005419:Flavonoids / Q000302:isolation & purification*; D035264:Flowers / Q000737:chemistry*; D010636:Phenols / Q000302:isolation & purification*; D012431:Rutin / Q000302:isolation & purification*; D029645:Thymelaeaceae / Q000737:chemistry*
D005419:Flavonoids; D010636:Phenols; C513882:nicotiflorin; D012431:Rutin