title: Expanded multilocus sequence typing for burkholderia species.
abstract: PCR primers targeting loci in the current Burkholderia cepacia complex multilocus sequence typing scheme were redesigned to (i) more reliably amplify these loci from B. cepacia complex species, (ii) amplify these same loci from additional Burkholderia species, and (iii) enable the use of a single primer set per locus for both amplification and DNA sequencing.
authors.affiliation: Department of Pediatrics and Communicable Diseases, University of Michigan Medical School, Ann Arbor, 48109-0646, USA.
authors.forename: Theodore
authors.initials: T
authors.lastname: Spilker
authors.forename: Adam
authors.initials: A
authors.lastname: Baldwin
authors.forename: Amy
authors.initials: A
authors.lastname: Bumford
authors.forename: Chris G
authors.initials: CG
authors.lastname: Dowson
authors.forename: Eshwar
authors.initials: E
authors.lastname: Mahenthiralingam
authors.forename: John J
authors.initials: JJ
authors.lastname: LiPuma
mesh_terms: D015373:Bacterial Typing Techniques / Q000379:methods*; D019117:Burkholderia / Q000145:classification* / Q000235:genetics*; D016000:Cluster Analysis; D016172:DNA Fingerprinting / Q000379:methods*; D017931:DNA Primers / Q000235:genetics; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics*; D010802:Phylogeny; D017422:Sequence Analysis, DNA
chemical_list: D017931:DNA Primers; D004269:DNA, Bacterial