Determination of dehydroevodiamine in Evodia rutaecarpa (Juss.) Benth by high performance liquid chromatography and classification of the samples by using hierarchical clustering analysis.
A simple, sensitive and accurate liquid chromatographic method with photodiode-array detection was developed for determination of dehydroevodiamine with detection wavelength at 368 nm and column temperature at 30 degrees C. The separation was carried out on an Agilent Zorbax SB-C(18) column (250 mm x 4.6 mm, 5 microm) together with a C(18) guard column. The mobile phase was acetonitrile-water (containing 30 mM sodium acetate trihydrate and 0.15% acetic acid) in the ratio of 30:70 (v/v) delivered at a flow rate of 1 mL/min. Excellent linear behavior was observed over the concentration range investigated, with correlation coefficient (R(2))=0.9998. This validated method was applied to determine the contents of dehydroevodiamine in 36 samples from different regions of China, and hierarchical clustering analysis was firstly used to classify and differentiate Evodia rutaecarpa samples. The analysis is specific and can be successfully applied to analyze E. rutaecarpa which is helpful for quality control of the herb.
Zhao
Yang
Y
The Research Center for Quality Control of Natural Medicine, Guizhou Normal University, Guiyang 550001, China.
Zhou
Xin
X
Chen
Hua-Guo
HG
Gong
Xiao-Jian
XJ
Cai
Zong-Wei
ZW
Zhou
Chan-Yuan
CY
D000097:Acetonitriles; D000470:Alkaloids / Q000032:analysis* / Q000737:chemistry; D002681:China; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D016000:Cluster Analysis; D032086:Evodia / Q000737:chemistry* / Q000145:classification; D005638:Fruit; D010936:Plant Extracts / Q000737:chemistry*; D015203:Reproducibility of Results
D000097:Acetonitriles; D000470:Alkaloids; D010936:Plant Extracts; C028633:dehydroevodiamine; C032159:acetonitrile