Iloprost, a prostacyclin (PGI2) analogue, reduces liver injury in hepatic ischemia-reperfusion in rats.
OBJECTIVE
To evaluate the effects of iloprost a prostacyclin analogue on the hepatic IR injury in rats.


METHODS
Forty male Sprague-Dawley rats (250-300 g) were divided into four groups each containing 10 rats;(1)--controls: data from unmanipulated animals; (2) sham group: rats subjected to the surgical procedure, except for liver I/R, and given saline; (3) I/R group: rats that underwent liver ischemia for 45 min followed by reperfusion for 45 min; (4) IR/ Iloprost group: rats pretreated with iloprost (10 microg kg-1, i.v). Liver tissues were taken to determine SOD, CAT, GSH, and MDA levels and for biochemical and histological evaluation.


RESULTS
The plasma ALT and AST levels were increased in group 3 than in group 4. MDA values and the liver injury score decreased, while the SOD, CAT, and GSH values increased in group 4 compared to group 3. In group 3, hepatocytes were swollen with marked vacuolization. In group 4, there were regular sinusoidal structures with normal morphology without any signs of congestion.


CONCLUSIONS
We demonstrated hepatoprotective effects of iloprost against severe ischemia and reperfusion injury in rat liver.
Gedik
Ercan
E
Department of General Surgery, Medical School, Dicle University, Diyarbakir, Turkey. ercan.gedik@yohoo.com.br
Girgin
Sadullah
S
Obay
Basra Deniz
BD
Ozturk
Hayrettin
H
Ozturk
Hulya
H
Buyukbayram
Huseyin
H
D000704:Analysis of Variance; D000818:Animals; D004195:Disease Models, Animal; D004353:Drug Evaluation, Preclinical; D016285:Iloprost / Q000627:therapeutic use*; D008099:Liver / Q000098:blood supply* / Q000187:drug effects; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects*; D010975:Platelet Aggregation Inhibitors / Q000627:therapeutic use*; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000188:drug therapy* / Q000517:prevention & control
D010975:Platelet Aggregation Inhibitors; D016285:Iloprost