Photochemical control of FlAsH labeling of proteins.
Spatiotemporal control of protein fluorescence is a powerful tool in tracking protein movements within cells. Here we report an approach to using genetically encoded photo-caged amino acids to control labeling protein tetracysteine tags with biarsenical fluorescein dyes (FlAsH).
Wilkins
Bryan J
BJ
Department of Chemistry and Biochemistry, University of Maryland, College Park, MD 20742, USA.
Yang
Xu
X
Cropp
T Ashton
TA
D000596:Amino Acids / Q000737:chemistry; D000818:Animals; D002625:Chemistry, Organic / Q000379:methods; D003545:Cysteine / Q000737:chemistry*; D015195:Drug Design; D004591:Electrophoresis, Polyacrylamide Gel; D019793:Fluorescein / Q000138:chemical synthesis* / Q000494:pharmacology; D005456:Fluorescent Dyes / Q000138:chemical synthesis* / Q000494:pharmacology; D008968:Molecular Conformation; D015394:Molecular Structure; D010777:Photochemistry / Q000379:methods; D011506:Proteins / Q000737:chemistry; D012549:Schistosoma japonicum / Q000378:metabolism
D000596:Amino Acids; D005456:Fluorescent Dyes; D011506:Proteins; D003545:Cysteine; D019793:Fluorescein