GC-MS/MS multi-residue method for the determination of organochlorine pesticides, polychlorinated biphenyls and polybrominated diphenyl ethers in human breast tissues.
In this work, a multiresidue method for the quantification and confirmation of around 30 organohalogenated compounds in human breast tissue samples has been developed. Analytes tested included organochlorine (OC) (pesticides and polychlorinated biphenyls) and organobromine (OBr) (polybrominated diphenyl ether) compounds. The approach is based on a simple extraction with hexane, followed by a SPE clean-up using silica cartridges and final measurement by GC coupled to triple quadrupole MS. Analyses were performed in both ionizations, electron impact (EI) (selected reaction monitoring (SRM) mode) and negative chemical ionization (NCI) (selected ion recording (SIR) mode). Three isotopically labeled standards were added before extraction and used as surrogates: HCB-13C6, lindane-D6 and p,p'-DDE-D8. The method was validated in terms of accuracy, precision, LOQ and LOD and confirmation reliability, using breast tissue spiked at three concentration levels in the range 1-100 ng/g for OC compounds and at two levels 0.1 and 10 ng/g for OBr compounds (0.5 and 50 ng/g for BDE 209). The usefulness of the developed method was tested by the analysis of real human samples, giving as a result the detection of several OC and OBr compounds in different samples analyzed. The acquisition of at least two SRM transitions (in EI) or ions (in NCI) per analyte allowed positive findings to be confirmed by accomplishment of ion ratios between the quantification and the confirmation transitions or ions.
Medina
Cecilia M
CM
Research Institute for Pesticides and Water, University Jaume I, Castellón, Spain.
Pitarch
Elena
E
Portolés
Tania
T
López
Franciso J
FJ
Hernández
Félix
F
D001940:Breast / Q000737:chemistry*; D004785:Environmental Pollutants / Q000032:analysis*; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry / Q000295:instrumentation / Q000379:methods*; D055768:Halogenated Diphenyl Ethers / Q000032:analysis*; D006801:Humans; D006843:Hydrocarbons, Chlorinated / Q000032:analysis*; D010575:Pesticides / Q000032:analysis*; D011078:Polychlorinated Biphenyls / Q000032:analysis*; D015203:Reproducibility of Results
D004785:Environmental Pollutants; D055768:Halogenated Diphenyl Ethers; D006843:Hydrocarbons, Chlorinated; D010575:Pesticides; D011078:Polychlorinated Biphenyls