title: Proteomic analysis of the rat ovary following chronic low-dose exposure to 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD).
abstract: 2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) is a ubiquitously distributed endocrine-disrupting chemical and reproductive toxicant. In order to elucidate low-dose TCDD-mediated effects on reproductive or endocrine functions, female Sprague-Dawley rats were orally administered various concentrations (20, 50, or 125 ng/kg once weekly) TCDD for 29 wk. A proteomic analysis of the ovaries by two-dimensional gel electrophoresis and matrix-assisted laser desorption/ionization (MALDI) tandem mass spectrometry showed distinct changes in the levels of several proteins that are relevant markers of TCDD toxicity. Serum estradiol (E2) levels of TCDD-treated animals were markedly lower than control. There were no significant differences in bone mineral density (BMD) of femurs. The body weight of the 125-ng/kg TCDD group was significantly decreased relative to control and there was also a significant reduction in absolute and relative ovarian weights. Expressions of selenium binding protein 2, glutathione S-transferase mu type 3, Lrpap1 protein, NADPH, and peptidylprolyl isomerase D were upregulated, while prohibitin and N-ethylmaleimide-sensitive factor expression levels were downregulated. Data provide further insight into the mechanisms by which TCDD disrupts ovarian function by indicating which differential protein expressions following low-dose TCDD exposure.
authors.affiliation: Department of Occupational and Environmental Health, School of Public Health, Tianjin Medical University, Tianjin, China.
authors.forename: Xi
authors.initials: X
authors.lastname: Chen
authors.forename: Xiao-ming
authors.initials: XM
authors.lastname: Ma
authors.forename: Shi-wei
authors.initials: SW
authors.lastname: Ma
authors.forename: Pieter-Jan
authors.initials: PJ
authors.lastname: Coenraads
authors.forename: Chun-mei
authors.initials: CM
authors.lastname: Zhang
authors.forename: Jing
authors.initials: J
authors.lastname: Liu
authors.forename: Li-jun
authors.initials: LJ
authors.lastname: Zhao
authors.forename: Min
authors.initials: M
authors.lastname: Sun
authors.forename: Nai-jun
authors.initials: NJ
authors.lastname: Tang
mesh_terms: D000284:Administration, Oral; D000818:Animals; D015536:Down-Regulation; D004785:Environmental Pollutants / Q000008:administration & dosage / Q000633:toxicity*; D004958:Estradiol / Q000378:metabolism; D005260:Female; D005982:Glutathione Transferase / Q000378:metabolism; D007527:Isoenzymes / Q000378:metabolism; D026563:LDL-Receptor Related Protein-Associated Protein; D052066:N-Ethylmaleimide-Sensitive Proteins / Q000378:metabolism; D009249:NADP / Q000378:metabolism; D009929:Organ Size / Q000187:drug effects*; D010053:Ovary / Q000187:drug effects* / Q000378:metabolism*; D019696:Peptidylprolyl Isomerase / Q000378:metabolism; D000072317:Polychlorinated Dibenzodioxins / Q000008:administration & dosage / Q000633:toxicity*; D000091402:Prohibitins; D040901:Proteomics; D051381:Rats; D017207:Rats, Sprague-Dawley; D012097:Repressor Proteins / Q000378:metabolism; D051137:Selenium-Binding Proteins; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D015854:Up-Regulation
chemical_list: D004785:Environmental Pollutants; D007527:Isoenzymes; D026563:LDL-Receptor Related Protein-Associated Protein; D000072317:Polychlorinated Dibenzodioxins; D000091402:Prohibitins; D012097:Repressor Proteins; C000622413:Selenbp1 protein, rat; D051137:Selenium-Binding Proteins; D004958:Estradiol; D009249:NADP; D005982:Glutathione Transferase; C416969:glutathione transferase M3-3; D052066:N-Ethylmaleimide-Sensitive Proteins; D019696:Peptidylprolyl Isomerase