A novel jet-based nano-hydroxyapatite patterning technique for osteoblast guidance.
Surface topography is well known to play a crucial role in influencing cellular responses to an implant material and is therefore important in bone tissue regeneration. A novel jet-based patterning technique, template-assisted electrohydrodynamic atomization spraying, was recently devised to control precisely the surface structure as well as its dimensions. In the present study, a detailed investigation of this patterning process was carried out. A range of nano-hydroxyapatite (nHA) line-shaped patterns <20 microm in width were successfully deposited on a commercially pure Ti surface by controlling the flow of an nHA suspension in an electric field. In vitro studies showed that the nHA patterns generated are capable of regulating the human osteoblast cell attachment and orientation.
Li
Xiang
X
Department of Mechanical Engineering, University College London, Torrington's Place, London WC1E 7JE, UK.
Koller
Garrit
G
Huang
Jie
J
Di Silvio
Lucy
L
Renton
Tara
T
Esat
Minoo
M
Bonfield
William
W
Edirisinghe
Mohan
M
D001672:Biocompatible Materials / Q000737:chemistry; D002448:Cell Adhesion / Q000502:physiology; D002478:Cells, Cultured; D017886:Durapatite / Q000737:chemistry*; D048091:Guided Tissue Regeneration / Q000379:methods*; D006801:Humans; D008422:Materials Testing; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure*; D010006:Osteoblasts / Q000166:cytology* / Q000502:physiology*; D010316:Particle Size; D023822:Tissue Engineering / Q000379:methods*
D001672:Biocompatible Materials; D017886:Durapatite