Electrochemical immunosensor for prostate-specific antigen using self-assembled oligophenylethynylenethiol monolayer containing dendrimer.
Electrochemical immunosensor for prostate-specific antigen (PSA) was fabricated with a self-assembled 4-(2-(4-(acetylthio)phenyl)ethynyl)benzoic acid (APBA) as a bioreceptor. In order to enhance the electrochemical activity of PSA detection, poly(amidoamine) dendrimer was linked on the APBA self-assembled monolayer (SAM). The formation and electrical properties of the SAMs were investigated by surface plasmon resonance and cyclic voltammetry, respectively. The surface morphology of PSA sandwich complex onto the APBA SAM was studied by atomic force microscopy.
Namgung
Mi-Ok
MO
Interdisciplinary Program of Integrated Biotechnology, Sogang University, Seoul 121-742, Republic of Korea.
Jung
Seul-Ki
SK
Chung
Chan Moon
CM
Oh
Se-Young
SY
D000480:Alkynes / Q000378:metabolism; D015374:Biosensing Techniques / Q000379:methods*; D050091:Dendrimers / Q000737:chemistry; D004560:Electricity; D004987:Ethers / Q000378:metabolism; D007118:Immunoassay / Q000379:methods; D018625:Microscopy, Atomic Force; D017430:Prostate-Specific Antigen / Q000032:analysis*; D020349:Surface Plasmon Resonance
D000480:Alkynes; D050091:Dendrimers; D004987:Ethers; C436384:phenyleneethynylene; D017430:Prostate-Specific Antigen