title: A novel calcium oxalate crystal growth inhibitory protein from the seeds of Dolichos biflorus (L.).
abstract: Recurrence and persistent side effects of present day treatment for urolithiasis restrict their use, so an alternate, using phytotherapy is being sought. Dolichos biflorus seeds, which are used as dietary food in India, possess antilithiatic properties. In the present study, a novel dimeric antilithiatic protein (98 kDa) from its seeds was purified based on its ability to inhibit calcium oxalate crystallization in vitro. Amino acid analysis of Dolichos biflorus antilithiatic protein showed abundant acidic amino acids. The mascot search engine presented sequence similarity with a calcium binding protein, calnexin of Pisum sativum from the m/z data obtained by MALDI TOF mass spectrometer. Above results demonstrate the anticalcifying/antilithiatic nature of a novel protein from the seeds of Dolichos biflorus and thus open new vistas for using plant proteins as therapeutic agents to treat urolithiasis.
authors.affiliation: Department of Biotechnology and Bioinformatics, Jaypee University of Information Technology, Waknaghat, Solan 173215, India. rkchaudhary81@gmail.com
authors.forename: Rakesh K
authors.initials: RK
authors.lastname: Bijarnia
authors.forename: Tanzeer
authors.initials: T
authors.lastname: Kaur
authors.forename: Surinder K
authors.initials: SK
authors.lastname: Singla
authors.forename: Chanderdeep
authors.initials: C
authors.lastname: Tandon
mesh_terms: D000596:Amino Acids / Q000032:analysis; D000645:Ammonium Sulfate / Q000737:chemistry; D002129:Calcium Oxalate / Q000037:antagonists & inhibitors / Q000737:chemistry* / Q000378:metabolism; D037281:Calnexin / Q000737:chemistry; D002852:Chromatography, Ion Exchange; D003460:Crystallization; D031294:Dolichos / Q000737:chemistry*; D007700:Kinetics; D018532:Pisum sativum; D010449:Peptide Mapping; D008517:Phytotherapy / Q000379:methods; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D010940:Plant Proteins / Q000737:chemistry / Q000494:pharmacology; D012639:Seeds / Q000737:chemistry; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D052878:Urolithiasis / Q000378:metabolism / Q000517:prevention & control
chemical_list: D000596:Amino Acids; D010936:Plant Extracts; D010940:Plant Proteins; D037281:Calnexin; D002129:Calcium Oxalate; D000645:Ammonium Sulfate