Improvement of Stearidonic acid production in Oleaginous Saccharomyces cerevisiae.
Rat Delta6 desaturase was introduced in "oleaginous" Saccharomyces cerevisiae genetically modified by the snf2 disruption, DGA1 overexpression, and LEU2 expression to improve stearidonic acid (18:4n-3 (Delta6, 9, 12, 15)) production. When this transformed yeast was cultured in the presence of 0.7 g/l alpha-linolenic acid for 7 d, 0.4 g of alpha-linolenic acid was accumulated and 44 mg of stearidonic acid was produced, mainly as a triacylglycerol in the 1 liter broth.
Kimura
Kazuyoshi
K
Institute for Biological Resources and Functions, National Institute of Advanced Industrial Science and Technology, Tsukuba, Ibaraki, Japan. kykimura@ni.aist.go.jp
Tomita
Nao
N
Uemura
Hiroshi
H
Aki
Tsunehiro
T
Ono
Kazuhisa
K
Kamisaka
Yasushi
Y
D002849:Chromatography, Gas; D051048:Diacylglycerol O-Acyltransferase / Q000235:genetics; D015525:Fatty Acids, Omega-3 / Q000096:biosynthesis*; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics
D015525:Fatty Acids, Omega-3; D029701:Saccharomyces cerevisiae Proteins; C494880:DGA1 protein, S cerevisiae; D051048:Diacylglycerol O-Acyltransferase; C062895:stearidonic acid