title: Atmospheric pressure infrared (10.6 microm) laser desorption electrospray ionization (IR-LDESI) coupled to a LTQ Fourier transform ion cyclotron resonance mass spectrometer.
abstract: We present the implementation of a CO(2) laser emitting infrared laser irradiation at 10.6 microm onto the versatile atmospheric pressure ionization platform. Infrared laser desorption electrospray ionization (IR-LDESI) is demonstrated from liquid-state samples at atmospheric pressure with and without ESI postionization. Multiply charged proteins ranging in molecular mass from 8.6 to 17 kDa were detected from liquid-state samples without the addition of matrix.
authors.affiliation: W. M. Keck FT-ICR Mass Spectrometry Laboratory, Department of Chemistry, North Carolina State University, Raleigh, NC 27695, USA.
authors.forename: Jason S
authors.initials: JS
authors.lastname: Sampson
authors.forename: David C
authors.initials: DC
authors.lastname: Muddiman
mesh_terms: D000818:Animals; D002417:Cattle; D008970:Molecular Weight; D011506:Proteins / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000295:instrumentation / Q000379:methods*; D017550:Spectroscopy, Fourier Transform Infrared / Q000295:instrumentation / Q000379:methods*
chemical_list: D011506:Proteins