title: Immunisation of the equine uterus against Streptococcus equi subspecies zooepidemicus using an intranasal attenuated Salmonella vector.
abstract: Attenuated Salmonella enterica serovar Typhimurium MGN707, expressing the SzP protective protein of the MB9 serovar of Streptococcus equi subspecies zooepidemicus (SzP-MB9) was tested for its safety and efficacy as a nebulised intranasal vaccine against streptococcal uterine infections in mares. In a preliminary study, vaccinated mares (n=5) displayed serum, nasal and uterine responses (P<0.05) to S. Typhimurium lipopolysaccharide (St-LPS). Subsequently, vaccinated mares (expressor group, n=7), but not mares vaccinated with the vector only (control group, n=7), displayed significant increases in SzP-MB9 antibodies in serum, nasal and uterine washes (P<0.05). Assuming the uteri of all nine mares were free of streptococci prior to challenge with 6.3 x 10(9) colony forming units of S. e. zooepidemicus MB9, significantly fewer S. e. zooepidemicus were cultured from the uterine flushings of expressor-vaccinated mares (n=4) compared to control-vaccinated mares (n=5) (P<0.001). The only adverse reaction to vaccination was nasal haemorrhage in one mare.
authors.affiliation: Department of Animal and Veterinary Sciences and the Maine Agriculture and Forestry Experiment Station, University of Maine, Orono, ME 04469-5735, USA.
authors.forename: Robert C
authors.initials: RC
authors.lastname: Causey
authors.forename: Sergey C
authors.initials: SC
authors.lastname: Artiushin
authors.forename: Ian F
authors.initials: IF
authors.lastname: Crowley
authors.forename: James A
authors.initials: JA
authors.lastname: Weber
authors.forename: Alma D
authors.initials: AD
authors.lastname: Homola
authors.forename: Antoinette
authors.initials: A
authors.lastname: Kelley
authors.forename: Lisa A
authors.initials: LA
authors.lastname: Stephenson
authors.forename: H Michael
authors.initials: HM
authors.lastname: Opitz
authors.forename: Sarah
authors.initials: S
authors.lastname: Guilmain
authors.forename: John F
authors.initials: JF
authors.lastname: Timoney
mesh_terms: D000281:Administration, Intranasal; D000818:Animals; D005260:Female; D005822:Genetic Vectors / Q000235:genetics / Q000276:immunology; D006734:Horse Diseases / Q000276:immunology* / Q000517:prevention & control*; D006736:Horses; D011897:Random Allocation; D012486:Salmonella typhimurium / Q000235:genetics / Q000276:immunology*; D013290:Streptococcal Infections / Q000276:immunology / Q000517:prevention & control / Q000662:veterinary*; D018502:Streptococcus equi / Q000276:immunology*; D014599:Uterus / Q000382:microbiology; D014611:Vaccination / Q000662:veterinary*; D014613:Vaccines, Attenuated
chemical_list: D014613:Vaccines, Attenuated