title: A depletion strategy for improved detection of human proteins from urine.
abstract: With rapidly growing interest in the urine proteome, methods for reducing sample complexity are becoming increasingly important. Depletion strategies for removal of high-abundance proteins from human urine have not been reported. A commercial kit designed for depletion of abundant proteins from plasma was evaluated for removing top proteins from urine of patients with proteinuria. The number of low-abundance proteins identified in urine after depletion increased nearly 2.5-fold.
authors.affiliation: ARUP Institute for Clinical and Experimental Pathology, 500 Chipeta Way, Salt Lake City, UT 84132, USA.
authors.forename: Mark M
authors.initials: MM
authors.lastname: Kushnir
authors.forename: Peter
authors.initials: P
authors.lastname: Mrozinski
authors.forename: Alan L
authors.initials: AL
authors.lastname: Rockwood
authors.forename: David K
authors.initials: DK
authors.lastname: Crockett
mesh_terms: D001798:Blood Proteins / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification; D016022:Case-Control Studies; D002846:Chromatography, Affinity; D002853:Chromatography, Liquid; D015180:Electrophoresis, Gel, Two-Dimensional; D006801:Humans; D011507:Proteinuria; D020543:Proteome / Q000032:analysis*; D040901:Proteomics / Q000379:methods*; D011933:Reagent Kits, Diagnostic; D016622:Silver Staining; D053719:Tandem Mass Spectrometry; D014462:Ultrafiltration
chemical_list: D001798:Blood Proteins; D020543:Proteome; D011933:Reagent Kits, Diagnostic