Integration of differentiation signals during indirect flight muscle formation by a novel enhancer of Drosophila vestigial gene.
The gene vestigial (vg) plays a key role in indirect flight muscle (IFM) development. We show here that vg is controlled by the Notch anti-myogenic signaling pathway in myoblasts and is regulated by a novel 822 bp enhancer during IFM differentiation. Interestingly, this muscle enhancer is activated in developing fibers and in a small number of myoblasts before the fusion of myoblasts with the developing muscle fibers. Moreover, we show that this enhancer is activated by Drosophila Myocyte enhancing factor 2 (MEF2), Scalloped (SD) and VG but repressed by Twist, demonstrating a sensitivity to differentiation in vivo. In vitro experiments reveal that SD can directly bind this enhancer and MEF2 can physically interact with both SD and TWI. Cumulatively, our data reveal the interplay between different myogenic factors responsible for the expression of an enhancer activated during muscle differentiation.
Bernard
Frédéric
F
Equipe Génétique Moléculaire de la Différenciation, Institut Jacques Monod, CNRS-Université Paris Diderot, Paris, France.
Kasherov
Petar
P
Grenetier
Sabrina
S
Dutriaux
Annie
A
Zider
Alain
A
Silber
Joël
J
Lalouette
Alexis
A
D000818:Animals; D002454:Cell Differentiation / Q000502:physiology*; D002460:Cell Line; D029721:Drosophila Proteins / Q000235:genetics* / Q000378:metabolism; D004331:Drosophila melanogaster / Q000033:anatomy & histology / Q000196:embryology / Q000235:genetics; D004742:Enhancer Elements, Genetic; D005426:Flight, Animal; D018507:Gene Expression Regulation, Developmental; D009132:Muscles / Q000196:embryology / Q000502:physiology; D032446:Myoblasts / Q000166:cytology / Q000502:physiology; D018006:Myogenic Regulatory Factors / Q000235:genetics / Q000378:metabolism; D009687:Nuclear Proteins / Q000235:genetics* / Q000378:metabolism; D051880:Receptors, Notch / Q000235:genetics / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction / Q000502:physiology*; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D051879:Twist-Related Protein 1 / Q000235:genetics / Q000378:metabolism
D029721:Drosophila Proteins; C499409:Mef2 protein, Drosophila; D018006:Myogenic Regulatory Factors; C497759:N protein, Drosophila; D009687:Nuclear Proteins; D051880:Receptors, Notch; D011993:Recombinant Fusion Proteins; D014157:Transcription Factors; C497753:Twi protein, Drosophila; D051879:Twist-Related Protein 1; C087984:sd protein, Drosophila; C095064:vg protein, Drosophila