Contractility of diabetic human corpus cavernosum smooth muscle in response to serotonin mediated via Rho-kinase.
OBJECTIVE
Serotonin (5-hydroxytryptamine, 5-HT)-induced contraction and the involvement of RhoA/Rho-kinase pathway in the 5-HT-induced contraction was investigated isometrically in vitro in both diabetic and nondiabetic human corpus cavernosum (HCC) tissues.


METHODS
HCC tissues were obtained from 12 patients. The response to 5-HT (10(-9) to 10(-5) mol/l) was studied in isolated HCC tissues in the absence and in the presence of a Rho-kinase inhibitor (Y-27632).


RESULTS
Preincubation with Y-27632 attenuated maximum contractions induced by 5-HT in tissues of both nondiabetics and diabetics. When diabetic and nondiabetic groups were compared, no significant difference was seen in 5-HT-induced contraction alone, but in the presence of Y-27632, 5-HT-induced contraction was significantly higher in the diabetic group.


CONCLUSIONS
These results suggest that the Rho-kinase-mediated pathway plays an important role for 5-HT-induced contraction in diabetic corpus cavernosum tissues.
Murat
Nergis
N
Advanced Professional School of Health Sciences, Dokuz Eylul University, Medical Faculty, Izmir, Turkey.
Soner
Burak Cem
BC
Demir
Omer
O
Esen
Adil
A
Gidener
Sedef
S
D000577:Amides / Q000494:pharmacology; D003920:Diabetes Mellitus / Q000378:metabolism / Q000503:physiopathology*; D004305:Dose-Response Relationship, Drug; D006801:Humans; D066298:In Vitro Techniques; D007537:Isometric Contraction / Q000187:drug effects; D008297:Male; D009126:Muscle Relaxation / Q000187:drug effects; D009130:Muscle, Smooth / Q000378:metabolism / Q000503:physiopathology*; D010413:Penis / Q000378:metabolism / Q000503:physiopathology*; D011725:Pyridines / Q000494:pharmacology; D012701:Serotonin / Q000494:pharmacology* / Q000502:physiology; D054460:rho-Associated Kinases / Q000037:antagonists & inhibitors / Q000378:metabolism*
D000577:Amides; D011725:Pyridines; C108830:Y 27632; D012701:Serotonin; D054460:rho-Associated Kinases