title: Characterization of two alternative Interleukin(IL)-10 5'UTR mRNA sequences, induced by lipopolysaccharide (LPS) stimulation of peripheral blood mononuclear cells.
abstract: IL-10 production shows a broad-spectrum of individual response, suggesting a genetic component of approximately 75%. Different polymorphisms located close to, or within the IL-10 gene has been demonstrated to influence its transcription rate whereas the post-transcriptional regulation of IL-10 production has not well elucidated. The main responsible elements at this control level are both the 5'- and 3'-untranslated regions (UTR's) of mRNAs, and as the 3'-UTR regions are mainly involved in the stability and decay rate of mRNAs, the 5'-UTR regions mediate the binding rate of the molecule with ribosomal 40S subunit as a cis-acting element. Herein are report data on the identification of two IL10 mRNA that differ by the length of respective 5'UTR regions (160 and 288 nucleotides, respectively; EMBL accession nrs: EU751618 and EU751619) produced after stimulation of human blood samples with bacterial lipopolysaccharide (LPS). The longer 5'UTR is constitutively expressed in unstimulated PBMC cells cultured at 37 degrees C for 24h, while in LPS stimulated cells an additional IL-10 mRNA molecule, containing a shorter 5'UTR, is synthesized. RNADRAW software (http://www.rnadraw.com/) analysis have indicated that the secondary structures of the shorter 5'UTR IL-10 mRNA region is more available for the binding to the 40S ribosomal subunit. In conclusion, our data seem to suggest that LPS could influence the post-transcriptional control of IL-10 production inducing an alternative mRNA immediately available in response to the inflammatory stimulation.
authors.affiliation: Clinical Pathology, Section of General Pathology, Department of Pathobiology and Biomedical Methodology, University of Palermo, Italy.
authors.forename: Giusi Irma
authors.initials: GI
authors.lastname: Forte
authors.forename: Letizia
authors.initials: L
authors.lastname: Scola
authors.forename: Daniele
authors.initials: D
authors.lastname: Bellavia
authors.forename: Loredana
authors.initials: L
authors.lastname: Vaccarino
authors.forename: Maria
authors.initials: M
authors.lastname: Sanacore
authors.forename: Giorgia
authors.initials: G
authors.lastname: Sisino
authors.forename: Concetta
authors.initials: C
authors.lastname: Scazzone
authors.forename: Calogero
authors.initials: C
authors.lastname: Caruso
authors.forename: Rainer
authors.initials: R
authors.lastname: Barbieri
authors.forename: Domenico
authors.initials: D
authors.lastname: Lio
mesh_terms: D020121:5' Untranslated Regions; D001483:Base Sequence; D002478:Cells, Cultured; D005786:Gene Expression Regulation; D006801:Humans; D016753:Interleukin-10 / Q000235:genetics / Q000378:metabolism*; D007963:Leukocytes, Mononuclear / Q000187:drug effects / Q000378:metabolism*; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism*; D012045:Regulatory Sequences, Nucleic Acid
chemical_list: D020121:5' Untranslated Regions; D008070:Lipopolysaccharides; D012333:RNA, Messenger; D016753:Interleukin-10