In vitro toxicity of MTA compared with other primary teeth pulpotomy agents.
OBJECTIVE
The main goal of this work is to compare the In vitro toxicity of MTA with other primary teeth pulpotomy agents.


METHODS
The In vitro toxicity of MTA, calcium hydroxide, ferric sulphate solution, diluted formocresol and Buckley's formocresol were tested using MTT and Neutral Red Uptake cell viability assays. The results for MTA were compared to those obtained for the other substances using ANOVA and Tukey statistical tests (p < 0.05).


RESULTS
MTA had the lower in vitro toxicity and Buckley's formocresol, the higher, with statically significant difference.


CONCLUSIONS
Among the primary teeth pulpotomy agents tested, MTA showed the lower In vitro toxicity, standing as the most promising substitute to formocresol.
de Menezes
José Vitor Nogara Borges
JV
Pediatric Dentistry, Universidade Federal do Paraná, Curitiba, Brazil. jvmenezes@gmail.com
Takamori
Esther Rieko
ER
Bijella
Maria Francisca Thereza Borro
MF
Granjeiro
José Mauro
JM
D016475:3T3 Cells; D017607:Aluminum Compounds / Q000633:toxicity*; D000818:Animals; D017610:Calcium Compounds / Q000633:toxicity*; D002126:Calcium Hydroxide / Q000633:toxicity; D002462:Cell Membrane / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D004396:Coloring Agents; D004338:Drug Combinations; D005290:Ferric Compounds / Q000633:toxicity; D005566:Formocresols / Q000633:toxicity; D007928:Lethal Dose 50; D051379:Mice; D008928:Mitochondria / Q000187:drug effects; D009499:Neutral Red; D010087:Oxides / Q000633:toxicity*; D011672:Pulpotomy / Q000009:adverse effects; D012387:Root Canal Filling Materials / Q000633:toxicity*; D017640:Silicates / Q000633:toxicity*; D013778:Tetrazolium Salts; D013844:Thiazoles
D017607:Aluminum Compounds; D017610:Calcium Compounds; D004396:Coloring Agents; D004338:Drug Combinations; D005290:Ferric Compounds; D005566:Formocresols; D010087:Oxides; D012387:Root Canal Filling Materials; D017640:Silicates; D013778:Tetrazolium Salts; D013844:Thiazoles; C086631:mineral trioxide aggregate; D009499:Neutral Red; C031025:formocresol; C024823:ferric sulfate; C022616:thiazolyl blue; D002126:Calcium Hydroxide