Enzymatic assay of D-glucuronate using uronate dehydrogenase.
D-Glucuronate is a key metabolite in the process of detoxification of xenobiotics and in a recently constructed synthetic pathway to produce D-glucaric acid, a "top value-added chemical" from biomass. A simple and specific assay of D-glucuronate would be useful for studying these processes, but existing assays are either time-consuming or nonspecific. Using uronate dehydrogenase cloned from Agrobacterium tumefaciens, we developed an assay for D-glucuronate with a detection limit of 5 microM. This method was shown to be more suitable for a system with many interfering compounds than previous methods and was also applied to assays for myo-inositol oxygenase activity.
Moon
Tae Seok
TS
Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
Yoon
Sang-Hwal
SH
Tsang Mui Ching
Mary-Jane
MJ
Lanza
Amanda M
AM
Prather
Kristala L Jones
KL
D000445:Aldehyde Oxidoreductases / Q000378:metabolism*; D004926:Escherichia coli / Q000201:enzymology; D005965:Glucuronates / Q000032:analysis* / Q000378:metabolism
D005965:Glucuronates; C023280:uronate dehydrogenase; D000445:Aldehyde Oxidoreductases