3-Phenyllactic acid production by substrate feeding and pH-control in fed-batch fermentation of Lactobacillus sp. SK007.
3-Phenyllactic acid (PLA), which is produced by some strains of lactic acid bacteria (LAB), is a known antimicrobial agent with a broad spectrum. Batch and fed-batch fermentation by the strain Lactobacillus sp. SK007 for PLA production have been reported. With batch fermentation without pH-control, PLA production yield was 2.42 g L(-1). When fed-batch fermentation by Lactobacillus sp. SK007 was conducted in 3 L initial volume with pH-control at 6.0 and intermittent feeding, which was developed after fermentation for 12 h and every 2 h with 120 mL 100 g L(-1) PPA phenylpyruvic acid (PPA) and 50 mL 500 g L(-1) glucose each time, PLA production yield reached 17.38 g L(-1). The final conversion ratio of PPA to PLA was 51.1%, and the PLA production rate was 0.241 g L(-1) h(-1). This indicated that PPA was the ideal substrate for PLA fermentation production, and fed-batch fermentation with intermittent PPA feeding and pH-control was an effective approach to improve PLA production yield.
Mu
Wanmeng
W
State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, China. wmmu@jiangnan.edu.cn
Liu
Fengli
F
Jia
Jianghua
J
Chen
Chao
C
Zhang
Tao
T
Jiang
Bo
B
D005285:Fermentation; D006863:Hydrogen-Ion Concentration; D007773:Lactates / Q000378:metabolism*; D007778:Lactobacillus / Q000378:metabolism*; D010667:Phenylpyruvic Acids / Q000378:metabolism; D013696:Temperature; D013997:Time Factors
D007773:Lactates; D010667:Phenylpyruvic Acids; C017648:3-phenyllactic acid; C031606:phenylpyruvic acid