Involvement of the bax and bcl-2 system in the induction of germ cell apoptosis is correlated with the time of reperfusion after testicular ischemia in a rat model.
The objective of this study was to examine the relationship between time of reperfusion and bax/bcl-2-dependent germ cell apoptosis after testicular ischemia-reperfusion injury in the rat. In ischemic testis, bax/bcl-2 ratio did not change significantly, and the elevation of germ cell apoptosis was not marked; in the contralateral testis, germ cell apoptosis increased after 6 hours of reperfusion, achieved statistical significance after 24 hours, and decreased after 72 hours of reperfusion and was initiated by decreased bcl-2 messenger RNA levels and elevated bax/bcl-2 ratio within the first 6 hours of reperfusion.
Sukhotnik
Igor
I
Department of Pediatric Surgery, Bnai Zion Medical Center, The Bruce Rappaport Faculty of Medicine, Technion-Israel Institute of Technology, Haifa, Israel.
Voskoboinik
Katya
K
Department of Pediatric Surgery, Bnai Zion Medical Center, The Bruce Rappaport Faculty of Medicine, Technion-Israel Institute of Technology, Haifa, Israel.
Lurie
Michael
M
Department of Pathology, Bnai Zion Medical Center, The Bruce Rappaport Faculty of Medicine, Technion-Israel Institute of Technology, Haifa, Israel.
Bejar
Yakov
Y
Department of Pathology, Bnai Zion Medical Center, The Bruce Rappaport Faculty of Medicine, Technion-Israel Institute of Technology, Haifa, Israel.
Coran
Arnold G
AG
Department of Section of Pediatric Surgery, C.S. Mott Children's Hospital and University of Michigan Medical School, Ann Arbor, Michigan.
Mogilner
Jorge G
JG
Department of Pediatric Surgery, Bnai Zion Medical Center, The Bruce Rappaport Faculty of Medicine, Technion-Israel Institute of Technology, Haifa, Israel. Electronic address: Jorge.mogilner@b-zion.org.il.
D000818:Animals; D017209:Apoptosis / Q000235:genetics / Q000502:physiology*; D004195:Disease Models, Animal; D005854:Germ Cells / Q000378:metabolism / Q000473:pathology / Q000502:physiology*; D007511:Ischemia / Q000209:etiology / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D008297:Male; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D015424:Reperfusion / Q000534:rehabilitation; D015398:Signal Transduction / Q000235:genetics / Q000502:physiology; D013086:Spermatic Cord Torsion / Q000150:complications / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D013737:Testis / Q000098:blood supply / Q000378:metabolism / Q000473:pathology; D013997:Time Factors; D051028:bcl-2-Associated X Protein / Q000235:genetics / Q000378:metabolism / Q000502:physiology*
D019253:Proto-Oncogene Proteins c-bcl-2; D051028:bcl-2-Associated X Protein