Expression of Potato virus Y cytoplasmic inclusion protein in tobacco results in disorganization of parenchyma cells, distortion of epidermal cells, and induces mitochondrial and chloroplast abnormalities, formation of membrane whorls and atypical lipid accumulation.
Infection of plant cells by potyviruses induces the formation of cytoplasmic inclusions ranging in size from 200 to 1000 nm. To determine if the ability to form these ordered, insoluble structures is intrinsic to the potyviral cytoplasmic inclusion protein, we have expressed the cytoplasmic inclusion protein from Potato virus Y in tobacco under the control of the chrysanthemum ribulose-1,5-bisphosphate carboxylase small subunit promoter, a highly active, green tissue promoter. No cytoplasmic inclusions were observed in the leaves of transgenic tobacco using transmission electron microscopy, despite being able to clearly visualize these inclusions in Potato virus Y infected tobacco leaves under the same conditions. However, we did observe a wide range of tissue and sub-cellular abnormalities associated with the expression of the Potato virus Y cytoplasmic inclusion protein. These changes included the disruption of normal cell morphology and organization in leaves, mitochondrial and chloroplast internal reorganization, and the formation of atypical lipid accumulations. Despite these significant structural changes, however, transgenic tobacco plants were viable and the results are discussed in the context of potyviral cytoplasmic inclusion protein function.
Shand
Kylie
K
Centre for Tropical Crops and Biocommodities, Queensland University of Technology, 2 George Street, Brisbane, Queensland 4001, Australia.
Theodoropoulos
Christina
C
Stenzel
Deborah
D
Dale
James L
JL
Harrison
Mark D
MD
D000595:Amino Acid Sequence; D002462:Cell Membrane / Q000473:pathology / Q000648:ultrastructure; D002736:Chloroplasts / Q000473:pathology / Q000648:ultrastructure; D008563:Membrane Lipids / Q000378:metabolism; D008928:Mitochondria / Q000473:pathology / Q000648:ultrastructure; D008969:Molecular Sequence Data; D019441:Plant Epidermis / Q000648:ultrastructure; D018515:Plant Leaves / Q000648:ultrastructure / Q000821:virology; D017800:Potyvirus / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D014026:Nicotiana / Q000648:ultrastructure / Q000821:virology; D014764:Viral Proteins / Q000096:biosynthesis*
D008563:Membrane Lipids; D011993:Recombinant Fusion Proteins; D014764:Viral Proteins; C079046:cytoplasmic inclusion protein, Potato virus Y