Human papillomavirus genotype specificity of hybrid capture 2 low-risk probe cocktail.
A genotyping study of 285 Hybrid Capture 2 low-risk probe cocktail-positive specimens showed cross-reactivity with several untargeted human papillomavirus genotypes. Cross-reactivity was often clinically beneficial due to the detection of untargeted low-risk genotypes. A total of 8.4% of positive results, usually weak, were due to cross-reactivity with high-risk genotypes. Establishment of a gray zone is recommended.
Poljak
Mario
M
Institute of Microbiology and Immunology, University of Ljubljana, Slovenia. mario.poljak@mf.uni-lj.si
Kocjan
Bostjan J
BJ
Kovanda
Anja
A
Lunar
Maja M
MM
Lepej
Snjezana Zidovec
SZ
Planinic
Ana
A
Seme
Katja
K
Vince
Adriana
A
D000293:Adolescent; D000328:Adult; D003429:Cross Reactions; D004279:DNA, Viral / Q000235:genetics* / Q000302:isolation & purification*; D005260:Female; D005838:Genotype; D006801:Humans; D009693:Nucleic Acid Hybridization / Q000379:methods*; D015345:Oligonucleotide Probes / Q000235:genetics; D027383:Papillomaviridae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D030361:Papillomavirus Infections / Q000175:diagnosis*; D012680:Sensitivity and Specificity; D055815:Young Adult
D004279:DNA, Viral; D015345:Oligonucleotide Probes