Characterization of apolipoprotein A-I as a potential biomarker for cholangiocarcinoma.
Serum samples from 60 cholangiocarcinoma (CC), 60 benign diseases of hepatobiliary and 53 normal individuals were analysed by SELDI-TOF-MS (Surface Enhanced Laser Desorption/Ionization Time of Flight Mass Spectrometry). It was found that a 28 k m/z peak was significantly decreased in CC and retained discriminatory value between CC and normal group, also between CC and benign groups. Then 1-D, 2-D gel electrophoresis and tandem mass spectroscopy were employed to isolate and identify the protein that correlates with observed SELDI-TOF-MS (m/z) value. The results demonstrated that 28 k m/z peak was apolipoprotein A-I (ApoA-I) and its identity was further validated by immunodepletion and Western blotting analysis. Subsequently, it was inspiring found that the decreased level of ApoA-I analysed by enzyme linked immunosorbent assay was consistent with SELDI-TOF-MS analysis. Therefore, it suggested that ApoA-I could be a potential useful biomarker for CC.
Wang
X
X
Key laboratory for Cell Proliferation and Regulation Biology Ministry of Education; Universities' Confederated Institute of Proteomics, Beijing, China.
Dai
S
S
Zhang
Z
Z
Liu
L
L
Wang
J
J
Xiao
X
X
He
D
D
Liu
B
B
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D016632:Apolipoprotein A-I / Q000097:blood*; D001650:Bile Duct Neoplasms / Q000175:diagnosis*; D001653:Bile Ducts, Intrahepatic; D014408:Biomarkers, Tumor / Q000097:blood*; D015153:Blotting, Western; D018281:Cholangiocarcinoma / Q000175:diagnosis*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods
D016632:Apolipoprotein A-I; D014408:Biomarkers, Tumor