Rapid detection of microcystins in cells and water.
Global occurrence and concern about microcystin contamination in water has prompted the development of a range of detection methods for their identification and quantification. However, most protocols are relatively time consuming, expensive and require laboratory expertise. The production of robust and sensitive recombinant antibodies has facilitated the development of a lateral flow immunoassay (ImmunoStrip) which can rapidly detect microcystins and nodularins in the field with minimal equipment or processing. Here we evaluate the sensitivity and cross-reactivity of the commercially produced ImmunoStrip) and apply them to the detection of microcystins in laboratory cultures and natural samples. It was observed that while the ImmunoStrip) are marketed for the detection of 10 microg/l microcystin, all 7 microcystins and nodularin that were tested were detected below 1 microg/l. Furthermore, microcystins and nodularins were successfully detected in a range of laboratory cultures and samples from irrigation ponds.
Lawton
Linda A
LA
School of Pharmacy and Life Sciences, Robert Gordon University, St Andrew Street, Aberdeen AB25 1HG, UK. l.lawton@rgu.ac.uk
Chambers
Heather
H
Edwards
Christine
C
Nwaopara
Assumpta A
AA
Healy
Mike
M
D001427:Bacterial Toxins / Q000032:analysis* / Q000276:immunology; D007118:Immunoassay / Q000379:methods*; D057230:Limit of Detection; D052998:Microcystins / Q000032:analysis* / Q000276:immunology; D010456:Peptides, Cyclic / Q000032:analysis* / Q000276:immunology; D011237:Predictive Value of Tests; D011934:Reagent Strips; D015203:Reproducibility of Results; D014874:Water Pollutants, Chemical / Q000032:analysis* / Q000276:immunology
D001427:Bacterial Toxins; D052998:Microcystins; D010456:Peptides, Cyclic; D011934:Reagent Strips; D014874:Water Pollutants, Chemical; C063998:nodularin