QTL analysis of early stage heterosis for biomass in Arabidopsis.
The main objective of this study was to identify genomic regions involved in biomass heterosis using QTL, generation means, and mode-of-inheritance classification analyses. In a modified North Carolina Design III we backcrossed 429 recombinant inbred line and 140 introgression line populations to the two parental accessions, C24 and Col-0, whose F (1) hybrid exhibited 44% heterosis for biomass. Mid-parent heterosis in the RILs ranged from -31 to 99% for dry weight and from -58 to 143% for leaf area. We detected ten genomic positions involved in biomass heterosis at an early developmental stage, individually explaining between 2.4 and 15.7% of the phenotypic variation. While overdominant gene action was prevalent in heterotic QTL, our results suggest that a combination of dominance, overdominance and epistasis is involved in biomass heterosis in this Arabidopsis cross.
Meyer
Rhonda Christiane
RC
Department of Molecular Genetics, Leibniz Institute of Plant Genetics and Crop Plant Research, Corrensstrasse 3, 06466 Gatersleben, Germany. meyer@ipk-gatersleben.de
Kusterer
Barbara
B
Lisec
Jan
J
Steinfath
Matthias
M
Becher
Martina
M
Scharr
Hanno
H
Melchinger
Albrecht E
AE
Selbig
Joachim
J
Schurr
Ulrich
U
Willmitzer
Lothar
L
Altmann
Thomas
T
D017360:Arabidopsis / Q000235:genetics* / Q000254:growth & development; D018533:Biomass; D018745:Genome, Plant; D006823:Hybrid Vigor; D007178:Inbreeding; D010641:Phenotype; D040641:Quantitative Trait Loci