Performance evaluation of the automated NucliSens easyMAG nucleic acid extraction platform in comparison with QIAamp Mini kit from clinical specimens.
The performance of the NucliSens easyMAG platform for the extraction of nucleic acid from different clinical specimens was compared with a manual procedure. A total of 308 specimens were analyzed: 209 plasma samples collected for virus detection and quantification of cytomegalovirus (CMV) and Epstein-Barr virus (EBV) (n = 70), and 29 for HIV genotyping for drug resistance. Linearity of extraction was tested on dilution series of CMV and EBV; the correlation coefficient (R(2)) for standard curves based on repeated extraction runs was 0.99 for CMV and EBV. Inter- and intrarun variability was in accordance with previous studies, and the correlation between automated and manual extraction was very high. The concordant results were 95.7% for CMV and 100% for EBV. The results of sequence analysis for HIV drug resistance showed a concordance in 24 of 29 specimens. The NucliSens easyMAG is extremely easy to perform, is automated, and resulted in a strong reduction of hands-on time compared with manual protocol.
Perandin
Francesca
F
Department of Experimental and Applied Medicine, University of Brescia, Italy. perandin@med.unibs.it
Pollara
Patrizia C
PC
Gargiulo
Franco
F
Bonfanti
Carlo
C
Manca
Nino
N
D000704:Analysis of Variance; D001331:Automation; D003587:Cytomegalovirus / Q000302:isolation & purification; D004279:DNA, Viral / Q000302:isolation & purification*; D006678:HIV / Q000302:isolation & purification; D004854:Herpesvirus 4, Human / Q000302:isolation & purification; D006801:Humans; D025202:Molecular Diagnostic Techniques; D010949:Plasma / Q000821:virology; D012367:RNA, Viral / Q000302:isolation & purification*; D011933:Reagent Kits, Diagnostic; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D014777:Virus Diseases / Q000175:diagnosis* / Q000821:virology*
D004279:DNA, Viral; D012367:RNA, Viral; D011933:Reagent Kits, Diagnostic