Comparison of the Salmonella/microsome microsuspension assay with the new microplate fluctuation protocol for testing the mutagenicity of environmental samples.
The objective of this study was to compare the responses of the Salmonella/microsome microsuspension assay with the new microplate fluctuation protocol (MPF) for the evaluation of the mutagenic activity of environmental samples. Organic extracts of total particulate atmospheric air samples, surface waters, and effluents were tested in dose-response experiments. The assays were performed with strain TA98 in the absence and presence of S9 mix. Both protocols produced similar results, despite the fact that the maximum score of the MPF is limited to 48 wells, whereas in the regular plate assay it is possible to count up to 1,500 colonies using an automatic counter. Similar sensitivities based on the lowest dose that resulted in a positive response were obtained for both assays. The MPF procedure is less laborious (e.g., all-liquid format, use of multichannel pipettors) and allows for automation of the pipetting and dispensing steps, thus, reducing time of the analysis which is particularly important in environmental quality monitoring programs or in effect-directed analysis. The results show that the MPF procedure is a promising tool to test environmental samples for mutagenic activity.
Umbuzeiro
Gisela de Aragão
Gde A
CETESB-Cia de Tecnologia de Saneamento Ambiental, Av. Prof. Frederico, Hermann Jr., 345, São Paulo, Brazil. giselau@usp.br
Rech
Célia Maria
CM
Correia
Simone
S
Bergamasco
Ana Marcela
AM
Cardenette
Giselli Helena Lima
GH
Flückiger-Isler
Sini
S
Kamber
Markus
M
D001681:Biological Assay / Q000295:instrumentation / Q000379:methods*; D004784:Environmental Monitoring / Q000295:instrumentation / Q000379:methods*; D008861:Microsomes / Q000737:chemistry*; D009152:Mutagenicity Tests / Q000295:instrumentation / Q000379:methods*; D012475:Salmonella / Q000187:drug effects*; D014874:Water Pollutants, Chemical / Q000032:analysis / Q000633:toxicity*
D014874:Water Pollutants, Chemical