Synthesis and microcontact printing of dual end-functionalized mucin-like glycopolymers for microarray applications.
Click to view: Glycopolymers can be used to display glycans on microarrays in native-like architectures. The structurally uniform alkyne-terminated mucin mimetic glycopolymers (see picture; TR = fluorophore) were printed on azide-functionalized chips by microcontact printing in the presence of a copper catalyst. The surface-bound glycopolymers bind lectins in a ligand-specific manner.
Godula
Kamil
K
Department of Chemistry, University of California and The Molecular Foundry, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA.
Rabuka
David
D
Nam
Ki Tae
KT
Bertozzi
Carolyn R
CR
D005456:Fluorescent Dyes / Q000737:chemistry; D006023:Glycoproteins / Q000737:chemistry; D037102:Lectins / Q000737:chemistry*; D046228:Microarray Analysis; D009077:Mucins / Q000737:chemistry*; D011134:Polysaccharides / Q000138:chemical synthesis* / Q000737:chemistry; D013499:Surface Properties
D005456:Fluorescent Dyes; D006023:Glycoproteins; D037102:Lectins; D009077:Mucins; D011134:Polysaccharides