Chromatographic fingerprint analysis of Pycnogenol dietary supplements.
The bark of maritime pine (Pinus pinaster Aiton) has been widely used as a remedy for various degenerative diseases. A standard high-performance liquid chromatographic (HPLC) procedure for Pycnogenol analysis is a method specified in the United States Pharmacopeia (USP) monograph, which requires measurement of peak areas and identification of four components of the extract: caffeic acid, catechin, ferulic acid, and taxifolin. In this study, a fingerprint analysis using an HPLC method based on the USP monograph has been developed to provide additional qualitative information for the analysis of Pycnogenol-containing dietary supplements (PDS). Twelve commercially available PDS samples were purchased and analyzed along with a standard Pycnogenol extract. Their chromatographic fingerprints were analyzed using principal component analysis. The results showed that two of the samples were not consistent with the standard reference Pycnogenol extract. One contained other active ingredients in addition to Pycnogenol, and the other may have resulted from a quality control issue in manufacturing.
Chen
Pei
P
U.S. Department of Agriculture, Agricultural Research Service, Beltsville Human Nutrition Research Center, Food Composition and Method Development Laboratory, 161, BARC-East, Beltsville, MD 20705, USA. pei.chen@ars.usda.gov
Song
Fenhong
F
Lin
Long-Ze
LZ
D002851:Chromatography, High Pressure Liquid / Q000379:methods* / Q000592:standards / Q000706:statistics & numerical data; D019587:Dietary Supplements / Q000032:analysis* / Q000592:standards; D005419:Flavonoids / Q000032:analysis* / Q000592:standards; D005504:Food Analysis / Q000379:methods* / Q000592:standards / Q000706:statistics & numerical data; D028223:Pinus / Q000737:chemistry; D010936:Plant Extracts; D025341:Principal Component Analysis; D012015:Reference Standards; D021241:Spectrometry, Mass, Electrospray Ionization
D005419:Flavonoids; D010936:Plant Extracts; C024070:pycnogenols