title: Polyelectrolyte multilayer films functionalized with peptides for promoting osteoblast functions.
abstract: Layer-by-layer deposition of polyelectrolyte multilayer (PEM) thin films has recently been applied to biomaterial applications. This simple and versatile technique provides a wide variety of potential utilization by insertion of biomolecules such as cell adhesion peptides. In this work dual peptides containing RGD (a cell-binding domain) and LHRRVKI (a heparin-binding domain) were immobilized onto polystyrene by the PEM technique and the effects on osteoblast cell culture were investigated. These peptides were conjugated to the amino groups of poly(allylamine hydrochloride) and then adsorbed onto the top of a 10 layer poly(allylamine hydrochloride)/poly(acrylic acid) film assembled at either pH 2.0 or pH 6.5. Osteoblasts, isolated from neonatal rat calvariae, were then seeded and cultured on the peptide-conjugated surfaces. We found that the cells adhered and grew better on the RGD-conjugated PEM films. The osteoblasts exhibited a better differentiated phenotype on the pH 2.0 films than the pH 6.5 films with respect to calcium deposition. The incorporation of LHRRVKI did not support cell adhesion, growth and matrix mineral deposition. Our results showed that the efficacy of RGD conjugation on osteoblast behavior was affected by the base PEM film.
authors.affiliation: Department of Chemical Engineering, National Taiwan University, No. 1, Roosevelt Road, Section 4, Taipei 106, Taiwan. weibortsai@ntu.edu.tw
authors.forename: Wei-Bor
authors.initials: WB
authors.lastname: Tsai
authors.forename: Rita Pei-Yeh
authors.initials: RP
authors.lastname: Chen
authors.forename: Kuang-Ling
authors.initials: KL
authors.lastname: Wei
authors.forename: Yi-Ru
authors.initials: YR
authors.lastname: Chen
authors.forename: Tai-Yan
authors.initials: TY
authors.lastname: Liao
authors.forename: Hsuan-Liang
authors.initials: HL
authors.lastname: Liu
authors.forename: Juin-Yih
authors.initials: JY
authors.lastname: Lai
mesh_terms: D000499:Allylamine / Q000737:chemistry; D020816:Amino Acid Motifs; D000818:Animals; D002118:Calcium / Q000378:metabolism; D002448:Cell Adhesion; D018929:Cell Culture Techniques / Q000379:methods*; D002478:Cells, Cultured; D020099:Coated Materials, Biocompatible / Q000737:chemistry* / Q000378:metabolism; D008422:Materials Testing; D009842:Oligopeptides / Q000737:chemistry / Q000378:metabolism; D010006:Osteoblasts / Q000166:cytology / Q000502:physiology*; D010455:Peptides / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D011108:Polymers / Q000737:chemistry*; D011137:Polystyrenes / Q000737:chemistry; D051381:Rats; D013499:Surface Properties
chemical_list: D020099:Coated Materials, Biocompatible; D009842:Oligopeptides; D010455:Peptides; D011108:Polymers; D011137:Polystyrenes; D000499:Allylamine; C047981:arginyl-glycyl-aspartic acid; D002118:Calcium