title: High specificity of quantitative methylation-specific PCR analysis for MGMT promoter hypermethylation detection in gliomas.
abstract: Normal brain tissue from 28 individuals and 50 glioma samples were analyzed by real-time Quantitative Methylation-Specific PCR (QMSP). Data from this analysis were compared with results obtained on the same samples by MSP. QMSP analysis demonstrated a statistically significant difference in both methylation level (P = .000009 Mann Whitney Test) and frequencies (P = .0000007, Z-test) in tumour samples as compared with normal brain tissues. Although QMSP and MSP showed similar sensitivity, the specificity of QMSP analysis was significantly higher (93%; CI95%: 84%-100%) as compared with MSP (64%; 95%CI: 46%-82%). Our results suggest that QMSP analysis may represent a powerful tool to identify glioma patients that will benefit from alkylating agents chemotherapy.
authors.affiliation: Oncology Laboratory, IRCCS Casa Sollievo della Sofferenza Hospital, 71013 San Giovanni Rotondo, Italy. pparrella@operapadrepio.it
authors.forename: Paola
authors.initials: P
authors.lastname: Parrella
authors.forename: Antonella
authors.initials: A
authors.lastname: la Torre
authors.forename: Massimiliano
authors.initials: M
authors.lastname: Copetti
authors.forename: Vanna M
authors.initials: VM
authors.lastname: Valori
authors.forename: Raffaela
authors.initials: R
authors.lastname: Barbano
authors.forename: Angelo
authors.initials: A
authors.lastname: Notarangelo
authors.forename: Michele
authors.initials: M
authors.lastname: Bisceglia
authors.forename: Antonietta Pia
authors.initials: AP
authors.lastname: Gallo
authors.forename: Teresa
authors.initials: T
authors.lastname: Balsamo
authors.forename: Maria Luana
authors.initials: ML
authors.lastname: Poeta
authors.forename: Massimo
authors.initials: M
authors.lastname: Carella
authors.forename: Domenico
authors.initials: D
authors.lastname: Catapano
authors.forename: Salvatore
authors.initials: S
authors.lastname: Parisi
authors.forename: Bruno
authors.initials: B
authors.lastname: Dallapiccola
authors.forename: Evaristo
authors.initials: E
authors.lastname: Maiello
authors.forename: Vincenzo
authors.initials: V
authors.lastname: D'Angelo
authors.forename: Vito Michele
authors.initials: VM
authors.lastname: Fazio
mesh_terms: D019540:Area Under Curve; D001921:Brain / Q000378:metabolism; D001923:Brain Chemistry; D019175:DNA Methylation; D015254:DNA Modification Methylases / Q000235:genetics*; D045643:DNA Repair Enzymes / Q000235:genetics*; D005260:Female; D005910:Glioma / Q000235:genetics*; D006801:Humans; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction / Q000379:methods*; D011401:Promoter Regions, Genetic; D012372:ROC Curve; D012680:Sensitivity and Specificity; D018709:Statistics, Nonparametric; D025521:Tumor Suppressor Proteins / Q000235:genetics*
chemical_list: D025521:Tumor Suppressor Proteins; D015254:DNA Modification Methylases; C515491:MGMT protein, human; D045643:DNA Repair Enzymes