title: Elicitation studies in cell suspension cultures of Cannabis sativa L.
abstract: Cannabis sativa L. plants produce a diverse array of secondary metabolites. Cannabis cell cultures were treated with biotic and abiotic elicitors to evaluate their effect on secondary metabolism. Metabolic profiles analysed by (1)H NMR spectroscopy and principal component analysis (PCA) showed variations in some of the metabolite pools. However, no cannabinoids were found in either control or elicited cannabis cell cultures. Tetrahydrocannabinolic acid (THCA) synthase gene expression was monitored during a time course. Results suggest that other components in the signaling pathway can be controlling the cannabinoid pathway.
authors.affiliation: Pharmacognosy Department/Metabolomics, Institute of Biology, Gorlaeus Laboratories, P.O. Box 9502, Leiden University, 2300 RA Leiden, The Netherlands.
authors.forename: Isvett Josefina
authors.initials: IJ
authors.lastname: Flores-Sanchez
authors.forename: Jaroslav
authors.initials: J
authors.lastname: Pec
authors.forename: Junni
authors.initials: J
authors.lastname: Fei
authors.forename: Young Hae
authors.initials: YH
authors.lastname: Choi
authors.forename: Jaroslav
authors.initials: J
authors.lastname: Dusek
authors.forename: Robert
authors.initials: R
authors.lastname: Verpoorte
mesh_terms: D000704:Analysis of Variance; D002188:Cannabis / Q000187:drug effects / Q000201:enzymology* / Q000378:metabolism; D018929:Cell Culture Techniques; D002478:Cells, Cultured; D003517:Cyclopentanes / Q000494:pharmacology; D013759:Dronabinol / Q000031:analogs & derivatives* / Q000378:metabolism; D019746:Intramolecular Oxidoreductases / Q000235:genetics / Q000378:metabolism; D055442:Metabolome / Q000187:drug effects; D055432:Metabolomics / Q000379:methods; D000432:Methanol / Q000737:chemistry; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods; D054883:Oxylipins / Q000494:pharmacology; D010368:Pectins / Q000494:pharmacology; D010940:Plant Proteins / Q000378:metabolism*; D025341:Principal Component Analysis / Q000379:methods; D014867:Water / Q000737:chemistry
chemical_list: D003517:Cyclopentanes; D054883:Oxylipins; D010940:Plant Proteins; D014867:Water; C016780:11-nor-delta(9)-tetrahydrocannabinol-9-carboxylic acid; C011006:jasmonic acid; D013759:Dronabinol; D010368:Pectins; D019746:Intramolecular Oxidoreductases; C100677:delta(1)-tetrahydrocannabinolic acid synthase; D000432:Methanol