title: An integrated genetic and cytogenetic map of the cucumber genome.
abstract: The Cucurbitaceae includes important crops such as cucumber, melon, watermelon, squash and pumpkin. However, few genetic and genomic resources are available for plant improvement. Some cucurbit species such as cucumber have a narrow genetic base, which impedes construction of saturated molecular linkage maps. We report herein the development of highly polymorphic simple sequence repeat (SSR) markers originated from whole genome shotgun sequencing and the subsequent construction of a high-density genetic linkage map. This map includes 995 SSRs in seven linkage groups which spans in total 573 cM, and defines approximately 680 recombination breakpoints with an average of 0.58 cM between two markers. These linkage groups were then assigned to seven corresponding chromosomes using fluorescent in situ hybridization (FISH). FISH assays also revealed a chromosomal inversion between Cucumis subspecies [C. sativus var. sativus L. and var. hardwickii (R.) Alef], which resulted in marker clustering on the genetic map. A quarter of the mapped markers showed relatively high polymorphism levels among 11 inbred lines of cucumber. Among the 995 markers, 49%, 26% and 22% were conserved in melon, watermelon and pumpkin, respectively. This map will facilitate whole genome sequencing, positional cloning, and molecular breeding in cucumber, and enable the integration of knowledge of gene and trait in cucurbits.
authors.affiliation: Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Sino-Dutch Joint Lab of Horticultural Genomics, Opening Lab of Genetic Improvement of Agricultural Crops of Ministry of Agriculture, Beijing, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Ren
authors.forename: Zhonghua
authors.initials: Z
authors.lastname: Zhang
authors.forename: Jinhua
authors.initials: J
authors.lastname: Liu
authors.forename: Jack E
authors.initials: JE
authors.lastname: Staub
authors.forename: Yonghua
authors.initials: Y
authors.lastname: Han
authors.forename: Zhouchao
authors.initials: Z
authors.lastname: Cheng
authors.forename: Xuefeng
authors.initials: X
authors.lastname: Li
authors.forename: Jingyuan
authors.initials: J
authors.lastname: Lu
authors.forename: Han
authors.initials: H
authors.lastname: Miao
authors.forename: Houxiang
authors.initials: H
authors.lastname: Kang
authors.forename: Bingyan
authors.initials: B
authors.lastname: Xie
authors.forename: Xingfang
authors.initials: X
authors.lastname: Gu
authors.forename: Xiaowu
authors.initials: X
authors.lastname: Wang
authors.forename: Yongchen
authors.initials: Y
authors.lastname: Du
authors.forename: Weiwei
authors.initials: W
authors.lastname: Jin
authors.forename: Sanwen
authors.initials: S
authors.lastname: Huang
mesh_terms: D002874:Chromosome Mapping / Q000379:methods*; D029773:Citrullus / Q000235:genetics; D003001:Cloning, Molecular; D018553:Cucumis sativus / Q000235:genetics*; D028464:Cucurbita / Q000235:genetics; D019662:Cucurbitaceae / Q000235:genetics; D020732:Cytogenetic Analysis; D018744:DNA, Plant; D017343:Genes, Plant; D008040:Genetic Linkage; D005819:Genetic Markers / Q000235:genetics; D018745:Genome, Plant; D017404:In Situ Hybridization, Fluorescence; D011110:Polymorphism, Genetic; D013045:Species Specificity
chemical_list: D018744:DNA, Plant; D005819:Genetic Markers