A comparative study on oxidative damage and distributions of perfluorooctane sulfonate (PFOS) in mice at different postnatal developmental stages.
Effects of perfluorooctane sulfonate (PFOS) on maleic dialdehyde (MDA) content, superoxide dismutase (SOD) activity and total antioxidation capability (T-AOC) were compared in mice at different postnatal developmental stages, and concentrations and distributions of PFOS in different tissues were measured simultaneously. The male and female mice at postnatal day (PD) 7, PD 14, PD 21, PD 28 and PD 35 were distributed randomly to dosage group (50 mg/kg body weight) and control group (0 mg/kg body weight). Mice were administered with PFOS by once subcutaneous injection. Subsequently, after 24 hr, MDA content, SOD activity and T-AOC in brain and liver were analyzed. The PFOS concentrations in blood, brain and liver were determined by high-performance liquid chromatography negative electrospray tandem mass spectrometry (LC-MS). PFOS induced degression of the body weights of mice evidently and increase of relative weights of liver. Meanwhile, it depressed the SOD activity and T-AOC in brain and liver. The concentrations and distribution percentages of PFOS in blood, brain and liver of mice were significantly different at various postnatal developmental stages. Achieved results in this study indicate that younger mice pups were more sensitive to PFOS exposure. In addition, significant distinctions in concentrations and distribution percentages of PFOS in various tissues were demonstrated in this study. The gender difference observed was greater in the older mice. Thus it is worth giving attention especially to adverse effects of PFOS on foetus and children.
Liu
Li
L
Division of Hygienic Toxicology, School of Public Health, China Medical University, Liaoning, China.
Liu
Wei
W
Song
Jinlan
J
Yu
Hongyao
H
Jin
Yihe
Y
Oami
Kazunori
K
Sato
Itaru
I
Saito
Norimitsu
N
Tsuda
Shuji
S
D017738:Alkanesulfonic Acids / Q000493:pharmacokinetics / Q000633:toxicity; D000818:Animals; D000831:Animals, Newborn; D015415:Biomarkers / Q000378:metabolism; D001835:Body Weight / Q000187:drug effects; D001921:Brain / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D002851:Chromatography, High Pressure Liquid; D004785:Environmental Pollutants / Q000493:pharmacokinetics / Q000633:toxicity; D005260:Female; D005466:Fluorocarbons / Q000493:pharmacokinetics / Q000633:toxicity; D048788:Growth and Development / Q000187:drug effects*; D007279:Injections, Subcutaneous; D015227:Lipid Peroxidation; D008099:Liver / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D051379:Mice; D009929:Organ Size / Q000187:drug effects; D018384:Oxidative Stress / Q000187:drug effects*; D021241:Spectrometry, Mass, Electrospray Ionization; D013482:Superoxide Dismutase / Q000378:metabolism; D053719:Tandem Mass Spectrometry; D014018:Tissue Distribution
D017738:Alkanesulfonic Acids; D015415:Biomarkers; D004785:Environmental Pollutants; D005466:Fluorocarbons; D008315:Malondialdehyde; C076994:perfluorooctane sulfonic acid; D013482:Superoxide Dismutase