title: A rapid resolution liquid chromatographic method for fingerprint analysis of raw and processed caowu (Aconitum kusnezoffii).
abstract: A sensitive and reliable rapid resolution liquid chromatographic (RRLC) method coupled with diode array detection has been developed for the fingerprint analysis of raw and processed caowu (Aconitum kusnezoffii). The RRLC fingerprints were established with a Zorbax Extend C18 analytical column (4.6 x 50 mm, 1.8 microm) and gradient elution. Analysis run time was <10 min. The method displayed good precision, stability, and repeatability in fingerprint analysis. Characteristic RRLC fingerprints of caowu were generated and used to assess the consistency and differences in the products. Raw and processed caowu from different sources were analyzed under the developed RRLC conditions, yielding contrasting RRLC fingerprints. Comparison of the RRLC fingerprints of processed and raw caowu indicated that the major constituents changed during processing. Meanwhile, a peak area ratio analysis method was applied to assess their chromatographic fingerprints. In characterizing the constituents of caowu, 11 major chromatographic peaks were identified by mass spectrometry and compared with reference standards and reference data. In summary, RRLC fingerprinting is a rapid and useful way to evaluate the quality of raw and processed caowu.
authors.affiliation: Chinese Academy of Medical Sciences & Peking Union Medical College, Institute of Materia Medica, Beijing 100050, People's Republic of China.
authors.forename: Yan-Ling
authors.initials: YL
authors.lastname: Qiao
authors.forename: Ying-Hao
authors.initials: YH
authors.lastname: Zhang
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.forename: Jin-Lan
authors.initials: JL
authors.lastname: Zhang
mesh_terms: D000157:Aconitine / Q000032:analysis; D031841:Aconitum / Q000737:chemistry*; D002853:Chromatography, Liquid / Q000379:methods* / Q000592:standards / Q000706:statistics & numerical data; D004365:Drugs, Chinese Herbal / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification; D013058:Mass Spectrometry; D015394:Molecular Structure; D012015:Reference Standards
chemical_list: D004365:Drugs, Chinese Herbal; D000157:Aconitine