title: Biochemical characterization of haloalkane dehalogenases DrbA and DmbC, Representatives of a Novel Subfamily.
abstract: This study focuses on two representatives of experimentally uncharacterized haloalkane dehalogenases from the subfamily HLD-III. We report biochemical characterization of the expression products of haloalkane dehalogenase genes drbA from Rhodopirellula baltica SH1 and dmbC from Mycobacterium bovis 5033/66. The DrbA and DmbC enzymes show highly oligomeric structures and very low activities with typical substrates of haloalkane dehalogenases.
authors.affiliation: Institute of Experimental Biology and National Center for Biomolecular Research, Masaryk University, Brno, Czech Republic.
authors.forename: Andrea
authors.initials: A
authors.lastname: Jesenská
authors.forename: Marta
authors.initials: M
authors.lastname: Monincová
authors.forename: Tána
authors.initials: T
authors.lastname: Koudeláková
authors.forename: Khomaini
authors.initials: K
authors.lastname: Hasan
authors.forename: Radka
authors.initials: R
authors.lastname: Chaloupková
authors.forename: Zbynek
authors.initials: Z
authors.lastname: Prokop
authors.forename: Arie
authors.initials: A
authors.lastname: Geerlof
authors.forename: Jirí
authors.initials: J
authors.lastname: Damborsky
mesh_terms: D001419:Bacteria / Q000201:enzymology*; D002942:Circular Dichroism; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D006867:Hydrolases / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*; D008969:Molecular Sequence Data; D009163:Mycobacterium bovis / Q000201:enzymology; D055503:Protein Multimerization; D017434:Protein Structure, Tertiary; D017422:Sequence Analysis, DNA; D013379:Substrate Specificity
chemical_list: D004269:DNA, Bacterial; D006867:Hydrolases; C046137:haloalkane dehalogenase