The augmenting activity of 6-(methylsulfinyl)hexyl isothiocyanate on cellular glutathione levels is less sensitive to thiol compounds than its cytotoxic activity.
We analyzed the effects of thiol compounds on the biological activities of 6-(methylsulfinyl)hexyl isothiocyanate (6-MITC). Thiol compounds abolished the cytotoxic activity of 6-MITC, but did not abolish its activity augmenting cellular total glutathione levels and gamma-glutamylcysteine ligase gene expression. Thiol compounds might play an important role in the augmentation of several significant biological activities by overcoming the inherent limitations of 6-MITC.
Kidachi
Yumi
Y
Department of Clinical Pharmacy, Aomori University, Aomori, Japan.
Noshita
Toshiro
T
Yamaguchi
Hideaki
H
Umetsu
Hironori
H
Fuke
Yoko
Y
Ryoyama
Kazuo
K
D000818:Animals; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects*; D005978:Glutathione / Q000378:metabolism*; D017879:Isothiocyanates / Q000494:pharmacology*; D051379:Mice; D013438:Sulfhydryl Compounds / Q000494:pharmacology*
C505386:6-(Methylsulfinyl)hexyl isothiocyanate; D017879:Isothiocyanates; D013438:Sulfhydryl Compounds; D005978:Glutathione