Influence of selenium dose on mercury distribution and retention in suckling rats.
It is well known that metal-metal interactions in the body are age-dependent. We studied the influence of increasing selenium (Se) doses on mercury (Hg) distribution and retention in the postnatal period in Hg-exposed suckling rats. Seven-day-old Wistar pups were pretreated with three different oral doses of Se as sodium selenite (6.45, 12.9 and 19.4 micromol Se kg(-1) b.w.) over 3 days. This was followed by simultaneous Se (as sodium selenite) and Hg (as mercury chloride) oral administration over 4 days. The molar ratio between Se and Hg given to pups was 1:1, 2:1 and 3:1, respectively. Mercury and Se were measured in brain, kidneys, liver, plasma, erythrocytes and urine of pups on the day after the last administration by atomic absorption spectrometry. Results showed that in all samples Se concentrations rose almost proportionally to the dose of Se given to pups. Mercury concentration in organs, plasma and urine decreased with higher oral doses of Se. However, Hg concentration in erythrocytes increased with increasing Se dose. There was evidently a redistribution of Hg from plasma to erythrocytes at higher ratio of Se:Hg. Approximately equimolar doses of Se and Hg are necessary to produce maximum uptake of Hg by plasma and liver and minimum retention of Hg in the kidney and erythrocytes.
Orct
Tatjana
T
Analytical Toxicology and Mineral Metabolism Unit, Institute for Medical Research and Occupational Health, Ksaverska cesta 2, PO Box 291, HR-10001 Zagreb, Croatia. torct@imi.hr
Lazarus
Maja
M
Jurasović
Jasna
J
Blanusa
Maja
M
Piasek
Martina
M
Kostial
Krista
K
D000818:Animals; D000833:Animals, Suckling; D001921:Brain / Q000378:metabolism*; D003404:Creatinine / Q000652:urine; D004305:Dose-Response Relationship, Drug; D004347:Drug Interactions; D004912:Erythrocytes / Q000378:metabolism; D005260:Female; D007668:Kidney / Q000378:metabolism*; D008099:Liver / Q000378:metabolism*; D008297:Male; D008628:Mercury / Q000008:administration & dosage / Q000097:blood / Q000378:metabolism*; D009929:Organ Size / Q000187:drug effects; D011247:Pregnancy; D051381:Rats; D017208:Rats, Wistar; D012643:Selenium / Q000008:administration & dosage / Q000097:blood / Q000378:metabolism*; D013997:Time Factors; D014018:Tissue Distribution
D003404:Creatinine; D008628:Mercury; D012643:Selenium