Headspace solid-phase microextraction gas chromatography-mass spectrometry analysis of Eupatorium odoratum extract as an oviposition repellent.
Headspace solid-phase microextraction (HS-SPME) coupled with gas chromatography-mass spectrometry (GC-MS) analysis was used to study volatile and semi-volatile compounds emitted by the Eupatorium odoratum (E. odoratum) extract. Variables of HS-SPME such as the type of SPME fiber, extraction time and temperature, incubation time, desorption time and temperature have been optimized. Optimized conditions were obtained by the use of divinylbenzene/carboxen/polydimethylsiloxane (DVB/CAR/PDMS) fiber, 5 min/20 min incubation/extraction time at 65 degrees C, 5 min desorption time at 260 degrees C. Using three different polar chromatographic columns to get retention index and mass spectrometry data, 99 volatile and semi-volatile compounds were tentatively identified in the E. odoratum extract. This study has identified the promising source of E. odoratum oviposition repellent.
Cui
Shufen
S
Department of Biological Applied Engineering, Shenzhen Polytechnic, Shenzhen 518055, China. shufencui@163.com
Tan
Shuo
S
Ouyang
Gangfeng
G
Jiang
Shihong
S
Pawliszyn
Janusz
J
D036604:Chromolaena / Q000737:chemistry*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D007302:Insect Repellents / Q000032:analysis* / Q000737:chemistry / Q000494:pharmacology; D010058:Oviposition / Q000187:drug effects; D010936:Plant Extracts / Q000032:analysis* / Q000737:chemistry / Q000494:pharmacology; D052617:Solid Phase Microextraction / Q000379:methods*; D014835:Volatilization
D007302:Insect Repellents; D010936:Plant Extracts