title: Subinhibitory concentrations of fluconazole increase the intracellular sodium content in both fluconazole-resistant and -sensitive Candida albicans strains.
abstract: Fluconazole-sensitive (SC 5314) and -resistant (clinical isolate 1173) Candida albicans strains were compared in terms of their osmotolerance and tolerance to toxic sodium cations. The two strains did not differ in their tolerance to high osmotic pressure in general but exhibited distinct sensitivities to sodium cations. Although the fluconazole-resistant 1173 strain contained, under all conditions tested, significantly lower intracellular amounts of Na+, it was much more sodium sensitive than the SC 5314 strain. The addition of subinhibitory concentrations of fluconazole to media supplemented with NaCl significantly influenced the growth of both strains and resulted in substantially elevated intracellular sodium concentrations compared with growth in medium containing NaCl but no fluconazole.
authors.affiliation: Department of Microbiology and Virology, Faculty of Natural Sciences, Comenius University, Mlynska dolina B-2, Bratislava, Slovakia.
authors.forename: Anna
authors.initials: A
authors.lastname: Kolecka
authors.forename: Yannick
authors.initials: Y
authors.lastname: Krauke
authors.forename: Helena
authors.initials: H
authors.lastname: Bujdakova
authors.forename: Hana
authors.initials: H
authors.lastname: Sychrova
mesh_terms: D000935:Antifungal Agents / Q000494:pharmacology*; D002176:Candida albicans / Q000737:chemistry* / Q000187:drug effects* / Q000254:growth & development; D003470:Culture Media / Q000737:chemistry; D003600:Cytosol / Q000737:chemistry*; D025141:Drug Resistance, Fungal; D015725:Fluconazole / Q000494:pharmacology*; D006801:Humans; D009997:Osmotic Pressure; D012964:Sodium / Q000032:analysis* / Q000494:pharmacology; D013696:Temperature
chemical_list: D000935:Antifungal Agents; D003470:Culture Media; D015725:Fluconazole; D012964:Sodium