title: A genome-wide scan for quantitative trait loci affecting serum glucose and lipids in a White Duroc x Erhualian intercross F(2) population.
abstract: Serum glucose and lipid levels are associated with diabetes mellitus and cardiovascular disease. The purpose of this study was to identify quantitative trait loci (QTL) for serum glucose and lipids in a White Duroc x Erhualian resource population. Serum glucose, glycosylated serum proteins (GSP), and serum lipid levels were measured in a total of 760 F(2) animals at 240 days. Strong positive correlations were observed between total cholesterol (TC) and low-density-lipoprotein cholesterol (LDL-C)/high-density-lipoprotein cholesterol (HDL-C). A whole-genome scan was performed with 194 microsatellites covering the pig genome across the entire resource population, revealing 2 QTL for serum glucose and 15 QTL for serum lipids. Of them, three 1% genome-wide significant QTL were identified for LDL-C, TC, and triglycerides (TG) in an adjacent region (67-73 cM) on chromosome 2 (SSC2), and the QTL for LDL-C showed the largest effect with a 95% confidence interval of 5 cM. Another 1% genome-wide significant QTL was found for LDL-C at 87 cM on SSC8. Other QTL showed 5% genome-wide significant or suggestive effects on SSC4, 5, 7, 9, 11, 14, and 15. In total, five significant QTL for serum lipids and a suggestive QTL for GSP on SSC4 were identified for the first time in pigs. Most of the identified QTL are homologous to the previously reported QTL for serum lipids in humans and mice. As correlated traits, QTL for TC and LDL-C were always located in the same genomic regions. The results shed new light on studies of human atherosclerosis and cardiovascular-related diseases.
authors.affiliation: Key Laboratory for Animal Biotechnology of Jiangxi Province and the Ministry of Agriculture of China, Jiangxi Agricultural University, Nanchang, China.
authors.forename: Rongrong
authors.initials: R
authors.lastname: Chen
authors.forename: Jun
authors.initials: J
authors.lastname: Ren
authors.forename: Wanbo
authors.initials: W
authors.lastname: Li
authors.forename: Xiang
authors.initials: X
authors.lastname: Huang
authors.forename: Xueming
authors.initials: X
authors.lastname: Yan
authors.forename: Bin
authors.initials: B
authors.lastname: Yang
authors.forename: Yinggong
authors.initials: Y
authors.lastname: Zhao
authors.forename: Yuanmei
authors.initials: Y
authors.lastname: Guo
authors.forename: Huirong
authors.initials: H
authors.lastname: Mao
authors.forename: Lusheng
authors.initials: L
authors.lastname: Huang
mesh_terms: D000818:Animals; D001786:Blood Glucose; D005260:Female; D055106:Genome-Wide Association Study; D006824:Hybridization, Genetic; D008055:Lipids / Q000097:blood*; D008297:Male; D040641:Quantitative Trait Loci; D013552:Swine / Q000235:genetics* / Q000378:metabolism*
chemical_list: D001786:Blood Glucose; D008055:Lipids