title: Effect of a biological response modifier on expression of CD14 receptor and tumor necrosis factor-alpha in Staphylococcus aureus-infected mammary glands at drying off.
abstract: Agents that increase natural protective mechanisms have been proposed for prevention and treatment of intramammary infections. The objectives of this study were to get an insight of innate immune mechanisms that occur during bovine mammary involution in both uninfected and chronically Staphylococcus aureus-infected glands and to describe the effects on those mechanisms of a single intramammary infusion of a LPS-based biological response modifier (BRM) at the end of lactation. Three groups of 12 cows, each one including 6 S. aureus-infected and 6 uninfected, were infused in two mammary quarters with BRM or placebo and sacrificed at 7, 14 and 21 d of involution. In uninfected and S. aureus-infected quarters treated with a BRM, the number of monocytes/macrophages detected with CD14 antibody was significantly higher (P<0.05) than in placebo-treated quarters at every sampling evaluation period. In uninfected quarters, the TNF-alpha staining area was not affected by BRM treatment. However, in infected quarters, the immunostained area for TNF-alpha was significantly higher than in uninfected quarters and BRM treatment was associated with increased staining at 21 d of involution.
authors.affiliation: Facultad de Ciencias Veterinarias, Universidad Nacional del Litoral, R. P. Kreder 2805, (3080) Esperanza, Santa Fe, Argentina.
authors.forename: B E
authors.initials: BE
authors.lastname: Dallard
authors.forename: C
authors.initials: C
authors.lastname: Baravalle
authors.forename: H H
authors.initials: HH
authors.lastname: Ortega
authors.forename: M
authors.initials: M
authors.lastname: Tumini
authors.forename: V R
authors.initials: VR
authors.lastname: Canavesio
authors.forename: V E
authors.initials: VE
authors.lastname: Neder
authors.forename: L F
authors.initials: LF
authors.lastname: Calvinho
mesh_terms: D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000188:drug therapy* / Q000276:immunology*; D002452:Cell Count; D005260:Female; D007113:Immunity, Innate / Q000187:drug effects; D007150:Immunohistochemistry; D007155:Immunologic Factors / Q000494:pharmacology*; D007774:Lactation / Q000276:immunology; D018950:Lipopolysaccharide Receptors / Q000378:metabolism*; D008070:Lipopolysaccharides / Q000494:pharmacology; D008264:Macrophages / Q000166:cytology / Q000276:immunology; D008321:Mammary Glands, Animal / Q000166:cytology / Q000187:drug effects / Q000276:immunology* / Q000382:microbiology*; D009000:Monocytes / Q000166:cytology / Q000276:immunology; D013203:Staphylococcal Infections / Q000188:drug therapy / Q000276:immunology / Q000662:veterinary*; D013211:Staphylococcus aureus / Q000472:pathogenicity; D013997:Time Factors; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*
chemical_list: D007155:Immunologic Factors; D018950:Lipopolysaccharide Receptors; D008070:Lipopolysaccharides; D014409:Tumor Necrosis Factor-alpha