title: Bactrocerin-1: a novel inducible antimicrobial peptide from pupae of oriental fruit fly Bactrocera dorsalis Hendel.
abstract: A novel antimicrobial peptide, Bactrocerin-1, was purified and characterized from an immunized dipteran insect, Bactrocera dorsalis. Bactrocerin-1 has 20 amino acid residues with a mass of 2,325.95 Da. The amino acid sequence of Bactrocerin-1 showed very high similarity to the active fragment (46V-65S-NH(2)) of Coleoptericin A. The composition of amino acid residues revealed that Bactrocerin-1 is a hydrophobic, positively charged, and Lys/Ile/Gly-rich peptide. Minimal growth inhibition concentration (MIC) measurements for synthesized Bactrocerin-1 showed a very broad spectrum of anti-microbial activity against Gram-positive bacteria, Gram-negative bacteria, and fungi. Bactrocerin-1 did not show hemolytic activity toward mouse red blood cells even at a concentration of 50 microM. Analysis of the Helical-wheel projection and the CD spectrum suggested that Bactrocerin-1 contains the amphipathic alpha-helix.
authors.affiliation: Zhejiang Institute of Subtropical Crops, Wenzhou, P.R. China.
authors.forename: Xiang-Li
authors.initials: XL
authors.lastname: Dang
authors.forename: Jin-Huan
authors.initials: JH
authors.lastname: Tian
authors.forename: Wan-Ying
authors.initials: WY
authors.lastname: Yang
authors.forename: Wen-Xian
authors.initials: WX
authors.lastname: Wang
authors.forename: Jun
authors.initials: J
authors.lastname: Ishibashi
authors.forename: Ai
authors.initials: A
authors.lastname: Asaoka
authors.forename: Hui-Yu
authors.initials: HY
authors.lastname: Yi
authors.forename: Yi-Feng
authors.initials: YF
authors.lastname: Li
authors.forename: Yang
authors.initials: Y
authors.lastname: Cao
authors.forename: Minoru
authors.initials: M
authors.lastname: Yamakawa
authors.forename: Shuo-Yang
authors.initials: SY
authors.lastname: Wen
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000890:Anti-Infective Agents / Q000494:pharmacology*; D002942:Circular Dichroism; D004591:Electrophoresis, Polyacrylamide Gel; D005658:Fungi / Q000187:drug effects; D006090:Gram-Negative Bacteria / Q000187:drug effects; D006094:Gram-Positive Bacteria / Q000187:drug effects; D019476:Insect Proteins / Q000302:isolation & purification / Q000494:pharmacology*; D051379:Mice; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D017386:Sequence Homology, Amino Acid; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D033621:Tephritidae / Q000737:chemistry* / Q000254:growth & development
chemical_list: D000890:Anti-Infective Agents; D019476:Insect Proteins; C543939:bactrocerin-1, Bactrocera dorsalis