Differential gene expression in black tiger shrimp, Penaeus monodon, following administration of oxytetracycline and oxolinic acid.
The intensification of shrimp farming systems has led to the spreading of a variety of bacterial and viral diseases that continue to plague the shrimp industry worldwide. Efforts to combat these pathogenic organisms include the use of immunostimulants, probiotics, vaccines and antibiotics. Although a few studies have already reported on the effects of various stimuli on shrimp, the effect of antibiotics, particularly on the changes in the shrimp transcriptomic profile have yet to be reported. Here we show that injecting shrimp with oxytetracycline and oxolinic acid alters the expression of genes in the black tiger shrimp, Penaeus monodon, lymphoid organ. These antibiotics, especially oxylinic acid, down-regulated the expression of a few immune-related genes, most notably penaeidin, proPO, clotting protein, profilin and whey acidic protein.
Fagutao
Fernand F
FF
Graduate School of Marine Science and Technology, Tokyo University of Marine Science and Technology, Minato-ku, Tokyo 108-8477, Japan.
Yasuike
Motoshige
M
Santos
Mudjekeewis D
MD
Ruangpan
Lila
L
Sangrunggruang
Kulvara
K
Tassanakajon
Anchalee
A
Takahashi
Yuikinori
Y
Ueno
Ryuji
R
Kondo
Hidehiro
H
Hirono
Ikuo
I
Aoki
Takashi
T
D000818:Animals; D000900:Anti-Bacterial Agents / Q000008:administration & dosage / Q000494:pharmacology; D000890:Anti-Infective Agents / Q000008:administration & dosage / Q000494:pharmacology; D016000:Cluster Analysis; D015870:Gene Expression / Q000187:drug effects*; D020869:Gene Expression Profiling; D008221:Lymphoid Tissue / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D010093:Oxolinic Acid / Q000008:administration & dosage / Q000494:pharmacology*; D010118:Oxytetracycline / Q000008:administration & dosage / Q000494:pharmacology*; D033561:Penaeidae / Q000235:genetics*; D010455:Peptides / Q000235:genetics; D051304:Profilins / Q000235:genetics
D000900:Anti-Bacterial Agents; D000890:Anti-Infective Agents; D010455:Peptides; D051304:Profilins; C109156:penaeidin 1; D010093:Oxolinic Acid; D010118:Oxytetracycline