Miniaturized side-viewing imaging probe for fluorescence lifetime imaging (FLIM): validation with fluorescence dyes, tissue structural proteins and tissue specimens.
We report a side viewing fibre-based endoscope that is compatible with intravascular imaging and fluorescence lifetime imaging microscopy (FLIM). The instrument has been validated through testing with fluorescent dyes and collagen and elastin powders using the Laguerre expansion deconvolution technique to calculate the fluorescence lifetimes. The instrument has also been tested on freshly excised unstained animal vascular tissues.
Elson
D S
DS
Institute of Biomedical Engineering and Department of Biosurgery and Surgical Technology, Imperial College London, Exhibition Road, London SW7 2AZ, UK.
Jo
J A
JA
Marcu
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