Life detection using glucose and tetrasaccharide enantiomer pairs.
A life-detection system based on the expectation that any viable organism will utilize stereoisomers of a given compound asymmetrically is examined. Aqueous extracts of common soil, Mars regolith simulant JSC Mars-1, and suspensions of E. coli and S. cerevisiae were incubated with stereoisomer pairs. The enantiomeric pairs were either D- and L-glucose or a pair of chiral tetrasaccharides. Following an incubation period of 10 days, stereoisomeric selectivity is detectable with the glucose pair by mass spectrometry in extracts made from soil at 0.5 g/ml, in extracts made from JSC Mars-1 at 2.5 g/ml, and in cell suspensions down to 1.0 x 10(7) cells/ml. For the tetrasaccharide pair, stereoisomeric selectivity was detected in extracts made from 0.5 g/ml or more of common soil but not in JSC Mars-1 simulant. The effective sensitivity in extracts was 2.5 x 10(7) cells/ml or better for the glucose pair and 5.0 x 10(8) cells/ml or better for the tetrasaccharide pair. The sensitivity of the glucose pair was such that it could detect life in samples that would be found to be devoid of organic matter by the GCMS system carried by the Viking landers. The results demonstrate the utility of the approach in the search for biological activity on Mars. However, sensitivity is a function of the enantiomer pair used, and this might also be different for hypothetical martian organisms. Therefore, it will be necessary to characterize additional stereoisomeric pairs and, ultimately, to include several in a single test environment.
Warmflash
David
D
Department of Biology and Biochemistry, University of Houston, Houston, Texas 77204-5001, USA.
Chu
Huanyi
H
Siefert
Johnathan
J
Fox
George E
GE
D018533:Biomass; D002021:Buffers; D004926:Escherichia coli / Q000378:metabolism; D018559:Exobiology / Q000379:methods; D005118:Extraterrestrial Environment; D008401:Gas Chromatography-Mass Spectrometry; D005947:Glucose / Q000032:analysis* / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D019369:Life; D013058:Mass Spectrometry; D008970:Molecular Weight; D010710:Phosphates / Q000737:chemistry; D012441:Saccharomyces cerevisiae / Q000378:metabolism; D012680:Sensitivity and Specificity; D012987:Soil / Q000032:analysis; D021241:Spectrometry, Mass, Electrospray Ionization; D013237:Stereoisomerism; D014867:Water / Q000737:chemistry
D002021:Buffers; D010710:Phosphates; D012987:Soil; D014867:Water; D005947:Glucose