title: Reverse transfection using gold nanoparticles.
abstract: Reverse transfection from a solid surface has the potential to deliver genes into various types of cell and tissue more effectively than conventional methods of transfection. We present a method for reverse transfection using a gold colloid (GC) as a nanoscaffold by generating nanoclusters of the DNA/reagent complex on a glass surface, which could then be used for the regulation of the particle size of the complex and delivery of DNA into nuclei. With this method, we have found that the conjugation of gold nanoparticles (20 nm in particle size) to the pEGFP-N1/Jet-PEI complex resulted in an increase in the intensity of fluorescence of enhanced green fluorescent protein (EGFP) (based on the efficiency of transfection) from human mesenchymal stem cells (hMSCs), as compared with the control without GC. In this manner, we constructed a method for reverse transfection using GC to deliver genes into the cells effectively.
authors.affiliation: National Institute of Advanced Industrial Science and Technology, 2-41-6 OMI, Koto-ku, Tokyo, 135-0064, Japan.
authors.forename: Shigeru
authors.initials: S
authors.lastname: Yamada
authors.forename: Satoshi
authors.initials: S
authors.lastname: Fujita
authors.forename: Eiichiro
authors.initials: E
authors.lastname: Uchimura
authors.forename: Masato
authors.initials: M
authors.lastname: Miyake
authors.forename: Jun
authors.initials: J
authors.lastname: Miyake
mesh_terms: D017979:Gold Colloid; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000378:metabolism; D053768:Metal Nanoparticles; D008856:Microscopy, Fluorescence; D036103:Nanotechnology; D010316:Particle Size; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D014162:Transfection / Q000379:methods*
chemical_list: D017979:Gold Colloid; D011994:Recombinant Proteins; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins