[Experimental study on effects of energy controllable steep pulses on cytoskeleton of human ovarian cancer cells SKOV3].
The aim of our study was to determine the effects of energy controllable steep pulse (ECSP) on the cytoskeleton of human ovarian cancer cells SKOV3. SKOV3 cells were divided into five groups under ECSP treatment with different parameters (frequency, pulse duration, peak value of voltage). The positive control group included SKOV3 cells treated with volchicine; the negative control group included SKOV3 cells subjected to sham-lightning stroke. Rhodamine-phalloidine was used to label microfilament directly. After using immunofluorescence to label microbules, we observed them by means of Confocal Laser Scanning Microscope. Making specimen and using electronmicroscope, we observed the ultramicrostructure of cystoskeleton. The results showed that ECSP-treated-SKOV3 cells lost their normal cystoskeleton network structure. There were obvious microfilament disaggregation, diffused skeleton protein, and disappearance of cystoskeleton network structure. Also noticeable were microbule disaggregation, reduction of pseudopod, obvious microfilament disaggregation, permutation disorder and structure disappearance. Moreover, this effect bears a direct relation with dosage.
Lin
Anping
A
Department of Gynaecology and Obstetrics of The Second Affiliated Hospital, Chongqing University of Medical Science, Chongqing 400010, China.
Hu
Lina
L
Tang
Yao
Y
Sun
Caixin
C
Mi
Yan
Y
Yao
Chenguo
C
D045744:Cell Line, Tumor; D003599:Cytoskeleton / Q000648:ultrastructure*; D004553:Electric Conductivity; D004574:Electromagnetic Fields; D018274:Electroporation; D005260:Female; D006801:Humans; D010051:Ovarian Neoplasms / Q000473:pathology*; D011674:Pulse