A simple and rapid method for measurement of (10)B-para-boronophenylalanine in the blood for boron neutron capture therapy using fluorescence spectrophotometry.
Background and Purpose; (10)B deriving from (10)B-para-boronophenylalanine (BPA) and (10)B-borocaptate sodium (BSH) have been detected in blood samples of patients undergoing boron neutron capture therapy (BNCT) using prompt gamma ray spectrometer or Inductively Coupled Plasma (ICP) method, respectively. However, the concentration of each compound cannot be ascertained because boron atoms in both molecules are the target in these assays. Here, we propose a simple and rapid method to measure only BPA by detecting fluorescence based on the characteristics of phenylalanine. Material and Methods; (10)B concentrations of blood samples from human or mice were estimated by the fluorescence intensities at 275 nm of a BPA excited by light of wavelength 257 nm using a fluorescence spectrophotometer. Results; The relationship between fluorescence to increased BPA concentration showed a positive linear correlation. Moreover, we established an adequate condition for BPA measurement in blood samples containing BPA, and the estimated (10)B concentrations of blood samples derived from BPA treated mice were similar between the values obtained by our method and those by ICP method. Conclusion; This new assay will be useful to estimate BPA concentration in blood samples obtained from patients undergoing BNCT especially in a combination use of BSH and BPA.
Kashino
Genro
G
Particle Radiation Oncology Research Centre, Research Reactor Institute, Kyoto University.
Fukutani
Satoshi
S
Suzuki
Minoru
M
Liu
Yong
Y
Nagata
Kenji
K
Masunaga
Shin-Ichiro
S
Maruhashi
Akira
A
Tanaka
Hiroki
H
Sakurai
Yoshinori
Y
Kinashi
Yuko
Y
Fujii
Noriko
N
Ono
Koji
K
D000818:Animals; D001681:Biological Assay / Q000379:methods*; D001774:Blood Chemical Analysis / Q000379:methods*; D001894:Borohydrides / Q000097:blood*; D001896:Boron Compounds / Q000097:blood*; D016754:Boron Neutron Capture Therapy / Q000379:methods*; D006801:Humans; D051379:Mice; D008809:Mice, Inbred C3H; D010649:Phenylalanine / Q000031:analogs & derivatives* / Q000097:blood; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence / Q000379:methods*; D013438:Sulfhydryl Compounds / Q000097:blood*
D001894:Borohydrides; D001896:Boron Compounds; D013438:Sulfhydryl Compounds; C014651:mercaptoundecahydrododecaborate; D010649:Phenylalanine; C033685:4-boronophenylalanine