Expanded multilocus sequence typing for burkholderia species.
PCR primers targeting loci in the current Burkholderia cepacia complex multilocus sequence typing scheme were redesigned to (i) more reliably amplify these loci from B. cepacia complex species, (ii) amplify these same loci from additional Burkholderia species, and (iii) enable the use of a single primer set per locus for both amplification and DNA sequencing.
Spilker
Theodore
T
Department of Pediatrics and Communicable Diseases, University of Michigan Medical School, Ann Arbor, 48109-0646, USA.
Baldwin
Adam
A
Bumford
Amy
A
Dowson
Chris G
CG
Mahenthiralingam
Eshwar
E
LiPuma
John J
JJ
D015373:Bacterial Typing Techniques / Q000379:methods*; D019117:Burkholderia / Q000145:classification* / Q000235:genetics*; D016000:Cluster Analysis; D016172:DNA Fingerprinting / Q000379:methods*; D017931:DNA Primers / Q000235:genetics; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics*; D010802:Phylogeny; D017422:Sequence Analysis, DNA
D017931:DNA Primers; D004269:DNA, Bacterial