title: Competitive RT-PCR Strategy for Quantitative Evaluation of the Expression of Tilapia (Oreochromis niloticus) Growth Hormone Receptor Type I.
abstract: Quantization of gene expression requires that an accurate measurement of a specific transcript is made. In this paper, a quantitative reverse transcription-polymerase chain reaction (RT-PCR) by competition for tilapia growth hormone receptor type I is designed and validated. This experimental procedure was used to determine the abundance of growth hormone receptor type I transcript in different tilapia tissues. The results obtained with this developed competitive RT-PCR were similar to real-time PCR results reported recently. This protocol provides a reliable alternative, but less expensive than real-time PCR to quantify specific genes.
  
  
  BACKGROUND
  The online version of this article (DOI:10.1007/s12575-009-9002-3) contains supplementary material, which is available to authorized users.
authors.affiliation: Aquatic Biotechnology Department, Animal Biotechnology Division, Center for Genetic Engineering and Biotechnology, P.O. Box 6162, Havana, 10 600 Cuba.
authors.forename: Alina
authors.initials: A
authors.lastname: Rodríguez-Mallon
authors.forename: Yamilet
authors.initials: Y
authors.lastname: Cárdenas
authors.forename: Juana María
authors.initials: JM
authors.lastname: Lugo
authors.forename: Aymé
authors.initials: A
authors.lastname: Oliva
authors.forename: Antonio
authors.initials: A
authors.lastname: Morales
authors.forename: Mario Pablo
authors.initials: MP
authors.lastname: Estrada