title: Functional analysis of transcription factors in Arabidopsis.
abstract: Transcription factors (TFs) regulate the expression of genes at the transcriptional level. Modification of TF activity dynamically alters the transcriptome, which leads to metabolic and phenotypic changes. Thus, functional analysis of TFs using 'omics-based' methodologies is one of the most important areas of the post-genome era. In this mini-review, we present an overview of Arabidopsis TFs and introduce strategies for the functional analysis of plant TFs, which include both traditional and recently developed technologies. These strategies can be assigned to five categories: bioinformatic analysis; analysis of molecular function; expression analysis; phenotype analysis; and network analysis for the description of entire transcriptional regulatory networks.
authors.affiliation: Research Institute of Genome-Based Biofactory, National Institute of Advanced Industrial Science and Technology (AIST), Central 4, Higashi, Tsukuba, Japan.
authors.forename: Nobutaka
authors.initials: N
authors.lastname: Mitsuda
authors.forename: Masaru
authors.initials: M
authors.lastname: Ohme-Takagi
mesh_terms: D017360:Arabidopsis / Q000235:genetics* / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000235:genetics* / Q000378:metabolism; D019295:Computational Biology; D030541:Databases, Genetic; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D053263:Gene Regulatory Networks; D010641:Phenotype; D018749:RNA, Plant / Q000235:genetics; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism
chemical_list: D029681:Arabidopsis Proteins; D018749:RNA, Plant; D014157:Transcription Factors