Overexpression transgenesis in mouse: pronuclear injection.
Pronuclear injection is a mode of DNA delivery for the production of transgenic animals. We present protocols for the preparation of DNA for microinjection from small or large plasmids and from BACs. We describe the production of one-cell mouse embryos, unfertile males, and pseudo-pregnant females. Finally, we detail the set up and procedures for the microinjection of one-cell embryos and their transfer into foster females.
Gardiner
Wendy J K
WJ
Mary Lyon Centre, Medical Research Council, Harwell, UK.
Teboul
Lydia
L
D000818:Animals; D030801:Animals, Genetically Modified / Q000196:embryology*; D002467:Cell Nucleus / Q000235:genetics*; D004247:DNA / Q000008:administration & dosage*; D004624:Embryo Transfer; D004622:Embryo, Mammalian / Q000166:cytology / Q000502:physiology; D005260:Female; D018014:Gene Transfer Techniques; D008297:Male; D051379:Mice; D008845:Microinjections / Q000379:methods*; D019076:Transgenes / Q000502:physiology*
D004247:DNA