title: Bifidobacterium mongoliense sp. nov., from airag, a traditional fermented mare's milk product from Mongolia.
abstract: Two novel micro-organisms, designated strains YIT 10443(T) and YIT 10738, were isolated from airag, a traditional fermented mare's milk from Mongolia. The two strains were Gram-positive-staining, non-motile, asporogenous, catalase-negative, facultatively anaerobic rods of various shapes. Comparative analyses of 16S rRNA and ClpC ATPase (clpC) gene sequences and the presence of fructose-6-phosphate phosphoketolase (F6PPK) demonstrated that the novel strains were members of the genus Bifidobacterium. On the basis of 16S rRNA gene sequence similarity, the type strains of Bifidobacterium minimum (96.6 %) and Bifidobacterium psychraerophilum (95.7 %) were the closest neighbours of the novel strains, and DNA-DNA reassociation values with these strains were found to be lower than 15 %. The phenotypic and genotypic features demonstrated that the two strains represent a single, novel Bifidobacterium species, for which the name Bifidobacterium mongoliense sp. nov. is proposed. The type strain is YIT 10443(T) (=JCM 15461(T) =DSM 21395(T)).
authors.affiliation: Yakult Central Institute for Microbiological Research, 1796 Yaho, Kunitachi, Tokyo 186-8650, Japan. koichi-watanabe@yakult.co.jp
authors.forename: Koichi
authors.initials: K
authors.lastname: Watanabe
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Makino
authors.forename: Masae
authors.initials: M
authors.lastname: Sasamoto
authors.forename: Yuko
authors.initials: Y
authors.lastname: Kudo
authors.forename: Junji
authors.initials: J
authors.lastname: Fujimoto
authors.forename: Shirchin
authors.initials: S
authors.lastname: Demberel
mesh_terms: D000251:Adenosine Triphosphatases / Q000235:genetics; D000818:Animals; D015373:Bacterial Typing Techniques; D001483:Base Sequence; D001644:Bifidobacterium / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology; D043302:Cultured Milk Products / Q000382:microbiology*; D004269:DNA, Bacterial / Q000032:analysis; D005260:Female; D005285:Fermentation; D005838:Genotype; D006736:Horses; D008969:Molecular Sequence Data; D008986:Mongolia; D009693:Nucleic Acid Hybridization; D010641:Phenotype; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013045:Species Specificity
chemical_list: D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S; D000251:Adenosine Triphosphatases