title: Characterization of a specific interaction between ADAM23 and cellular prion protein.
abstract: ADAMs are transmembrane proteins implicated in several biological functions, including cytokine and growth factor shedding, fertilization, muscle and nervous system development. Here, we show for the first time that ADAM23, which is predominantly expressed in the central nervous system, co-localizes with cellular prion protein (PrP(C)) at plasma membrane of mouse hippocampal neurons and neuroblastoma cells. Co-immunoprecipitation and pull-down assay showed a physical interaction between ADAM23 and both recombinant and endogenous PrP(C). Glycosylation seems to be not relevant to the observed interaction since both ADAM23 and PrP(C) recombinant proteins expressed in bacteria or extracted from eukaryotic cells treated with tunicamycin are still able to bind each other. In vitro binding assays also suggested that the disintegrin domain of ADAM23 is able to interact directly with PrP(C). Taken together, these findings point out PrP(C) as a novel molecular partner for ADAM23 in the nervous systems.
authors.affiliation: Department of Basic Pathology, Universidade Federal do Paraná, Centro Politécnico, 81531-990 Curitiba, PR, Brazil.
authors.forename: Michele D M
authors.initials: MD
authors.lastname: Costa
authors.forename: Katia S
authors.initials: KS
authors.lastname: Paludo
authors.forename: Giseli
authors.initials: G
authors.lastname: Klassen
authors.forename: Marilene H
authors.initials: MH
authors.lastname: Lopes
authors.forename: Adriana F
authors.initials: AF
authors.lastname: Mercadante
authors.forename: Vilma R
authors.initials: VR
authors.lastname: Martins
authors.forename: Anamaria A
authors.initials: AA
authors.lastname: Camargo
authors.forename: Lia S
authors.initials: LS
authors.lastname: Nakao
authors.forename: Silvio M
authors.initials: SM
authors.lastname: Zanata
mesh_terms: D051722:ADAM Proteins / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D002462:Cell Membrane / Q000378:metabolism; D002478:Cells, Cultured; D006031:Glycosylation; D006624:Hippocampus / Q000166:cytology; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism*; D009447:Neuroblastoma; D009474:Neurons / Q000378:metabolism; D018621:PrPC Proteins / Q000235:genetics / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D009419:Nerve Tissue Proteins; D018621:PrPC Proteins; D011993:Recombinant Fusion Proteins; D051722:ADAM Proteins; C114633:ADAM23 protein, human; C121259:Adam23 protein, mouse