Simultaneous determination of anti-diabetes/anti-obesity drugs by LC/PDA, and targeted analysis of sibutramine analog in dietary supplements by LC/MS/MS.
The safety of dietary supplements is questionable as there have been occasional reports of products contaminated with illegal adulterants. The present study was carried out to develop trustworthy methodologies to screen for six anti-diabetic drugs (phenformin, rosiglitazone, glipizide, glimepiride, glybenclamide and gliclazide) and six anti-obesity drugs (ephedrine, fenfluramine, T3, T4, fluoxetine and sibutramine) in dietary supplements. A simultaneous determination method of the 12 drugs by liquid chromatography coupled with a photodiode array (LC/PDA) was established and was validated for linearity (r(2) > 0.99), precision (RSD <13.3%), recoveries (88.8-115.9%) and reproducibility. Sibutramine and its analogs, N-desmethylsibutramine, were subject to further investigation by LC/MS/MS because they were one of the major illegal adulterants. Our proposed method to monitor illegal drug adulterations in dietary supplements using LC/PDA is a simple and reliable, and therefore applicable to routine drug-adulteration screening.
Kim
So Hyun
SH
College of Pharmacy and Research Institute of Pharmaceutical Sciences, Seoul National University, Seoul, Korea.
Lee
Jeongmi
J
Yoon
Taehyung
T
Choi
Jangduck
J
Choi
Dongmi
D
Kim
Deukjoon
D
Kwon
Sung Won
SW
D019440:Anti-Obesity Agents / Q000032:analysis*; D002853:Chromatography, Liquid / Q000379:methods*; D003503:Cyclobutanes / Q000737:chemistry*; D019587:Dietary Supplements / Q000032:analysis*; D007004:Hypoglycemic Agents / Q000032:analysis*; D053719:Tandem Mass Spectrometry / Q000379:methods*
D019440:Anti-Obesity Agents; D003503:Cyclobutanes; D007004:Hypoglycemic Agents; C058254:sibutramine