A genetic test for yeast two-hybrid bait competency using RanBPM.
We describe a simple genetic test for assessing the competency of Gal4-based baits prior to a yeast two-hybrid screen, which allows determination of whether a bait protein is expressed appropriately for an interaction to be detected. The novel test, based on interaction with the protein RanBPM, is easier and more predictive than other methods such as Western blotting, allowing identification of approximately 80% of incompetent baits prior to screening.
Tucker
Chandra L
CL
Department of Biology, Duke University, Durham, North Carolina 27708, USA. chandra@duke.edu
Peteya
Leslie A
LA
Pittman
Adrianne M C
AM
Zhong
Jing
J
D048868:Adaptor Proteins, Signal Transducing / Q000235:genetics*; D003598:Cytoskeletal Proteins / Q000235:genetics*; D004268:DNA-Binding Proteins / Q000235:genetics; D005069:Evaluation Studies as Topic; D005821:Genetic Techniques; D008722:Methods; D009687:Nuclear Proteins / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics; D014157:Transcription Factors / Q000235:genetics; D020798:Two-Hybrid System Techniques / Q000592:standards*
D048868:Adaptor Proteins, Signal Transducing; D003598:Cytoskeletal Proteins; D004268:DNA-Binding Proteins; C052130:GAL4 protein, S cerevisiae; D009687:Nuclear Proteins; C116005:Ran binding protein 9; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors