title: [Seroprevalence of Anaplasma marginale in cattle from Center-South Region of Paraná State, Brazil by a competitive ELISA test with recombinant MSP5-PR1 protein].
abstract: Anaplasma marginale prevalence was determined in 223 sera samples in 2-year old or older cattle, from the Center-Southern Region of the Paraná State, including Ponta Grossa, Guarapuava and Laranjeiras do Sul municipalities. A survey of antibodies IgG class against Anaplasma marginale was performed through a competitive immune absorbent assay (cELISA-PR1). From the 223 sera examined, 130 (58.74%) reacted to cELISA-PR1 test, suggesting an region of enzootic instability, with a significant percentage of animals susceptible to infection by A. marginale and potentially in risk to develop anaplasmosis. The kind of exploration in the property, the breeding and handling system, the presence of other animals (ovine/caprine, horses, wild animals), and means of commercialization of animals were analyzed. The statistical analysis showed that there were no significant differences among the analyzed variables.
authors.affiliation: Departamento de Medicina Veterinária Preventiva, Universidade Estadual de Londrina--UEL, Brazil.
authors.forename: Elizabete Regina Marangoni
authors.initials: ER
authors.lastname: Marana
authors.forename: Juliana Alves
authors.initials: JA
authors.lastname: Dias
authors.forename: Roberta Lemos
authors.initials: RL
authors.lastname: Freire
authors.forename: Josy Campanhã
authors.initials: JC
authors.lastname: Vicentini
authors.forename: Marilda Carlos
authors.initials: MC
authors.lastname: Vidotto
authors.forename: Odilon
authors.initials: O
authors.lastname: Vidotto
mesh_terms: D042322:Anaplasma marginale / Q000276:immunology*; D000818:Animals; D001425:Bacterial Outer Membrane Proteins; D001938:Brazil; D002417:Cattle / Q000097:blood*; D004797:Enzyme-Linked Immunosorbent Assay; D007074:Immunoglobulin G / Q000097:blood*; D011994:Recombinant Proteins; D016036:Seroepidemiologic Studies
chemical_list: D001425:Bacterial Outer Membrane Proteins; D007074:Immunoglobulin G; D011994:Recombinant Proteins; C111578:major surface protein 5, Anaplasma