Raman-assisted X-ray crystallography for the analysis of biomolecules.
In this chapter, we describe Raman microspectrophotometry applied to crystals of biomolecules. Raman spectra collected in crystallo provide structural information highly complementary to X-ray diffraction, relate the crystalline state to the solution state, and allow the identification of ligand-bound or intermediate states of macromolecules. Nonresonant Raman spectroscopy is particularly suitable to the study of macromolecular crystals, and therefore applies to a wide range of noncolored crystalline proteins. Practical issues related to the investigation of crystals by Raman microspectrophotometry are reviewed, and the current limitations are highlighted.
Bourgeois
Dominique
D
Institut de Biologie Structurale Jean-Pierre Ebel, 41 Rue Jules Horowitz, 38027, Grenoble Cedex, France. dominique.bourgeois@ibs.fr
Katona
Gergely
G
de Rosny
Eve
E
Carpentier
Philippe
P
D001704:Biopolymers / Q000032:analysis* / Q000302:isolation & purification; D003460:Crystallization; D018360:Crystallography, X-Ray / Q000379:methods*; D003625:Data Collection; D046912:Multiprotein Complexes / Q000032:analysis / Q000302:isolation & purification; D013059:Spectrum Analysis, Raman / Q000295:instrumentation / Q000379:methods*
D001704:Biopolymers; D046912:Multiprotein Complexes