title: Single-molecule mechanical unfolding of amyloidogenic beta2-microglobulin: the force-spectroscopy approach.
abstract: The recombinant production of a novel chimeric polyprotein is described. The new protein contains either wild-type beta(2)-microglobulin (beta(2)m) or its truncated variant (DeltaN6 beta(2)m) (see picture). Structural characterization is achieved by means of single-molecule force spectroscopy studies of specific beta(2)m regions which could be involved in amyloidogenesis.
authors.affiliation: National Nanotechnology Laboratory of INFM-CNR, Italian Institute of Technology, Via Arnesano 16, 73100 Lecce, Italy.
authors.forename: B
authors.initials: B
authors.lastname: Sorce
authors.forename: S
authors.initials: S
authors.lastname: Sabella
authors.forename: M
authors.initials: M
authors.lastname: Sandal
authors.forename: B
authors.initials: B
authors.lastname: Samorì
authors.forename: A
authors.initials: A
authors.lastname: Santino
authors.forename: R
authors.initials: R
authors.lastname: Cingolani
authors.forename: R
authors.initials: R
authors.lastname: Rinaldi
authors.forename: P P
authors.initials: PP
authors.lastname: Pompa
mesh_terms: D000686:Amyloidosis; D018625:Microscopy, Atomic Force; D015202:Protein Engineering; D017510:Protein Folding; D011994:Recombinant Proteins / Q000737:chemistry; D013050:Spectrometry, Fluorescence; D001613:beta 2-Microglobulin / Q000737:chemistry*
chemical_list: D011994:Recombinant Proteins; D001613:beta 2-Microglobulin