Characterization of glycoside hydrolase family 6 enzymes from Coprinopsis cinerea.
Recombinants, Cel6 from Coprinopsis cinerea (CcCel6)A, -B, and -C, were expressed by Escherichia coli. These enzymes produced cellobiose from phosphoric acid-swollen cellulose. When Avicel was used as the substrate, CcCel6A showed higher activity toward the substrate than CcCel6B or -C. In reaction with carboxymethylcellulose, CcCel6B and -C hydrolyzed the substrate, whereas CcCel6A failed to react.
Liu
Yuan
Y
Department of Environmental and Natural Resource Science, Faculty of Agriculture, Tokyo University of Agriculture and Technology, Fuchu, Tokyo, Japan.
Igarashi
Kiyohiko
K
Kaneko
Satoshi
S
Tonozuka
Takashi
T
Samejima
Masahiro
M
Fukuda
Kiyoharu
K
Yoshida
Makoto
M
D001483:Base Sequence; D001487:Basidiomycota / Q000201:enzymology*; D002855:Chromatography, Thin Layer; D017931:DNA Primers; D004591:Electrophoresis, Polyacrylamide Gel; D006026:Glycoside Hydrolases / Q000378:metabolism*; D013379:Substrate Specificity
D017931:DNA Primers; D006026:Glycoside Hydrolases