Development of square wave voltammetry method for the assessment of organophosphorus compound impact on the cholinesterase of Pheretima with 2,6-dichloroindophenol as a redox indicator.
A square wave voltammetry method was developed for the assessment of organophosphorus (OPs) compound impact on the cholinesterase of Pheretima with 2,6-dichloroindophenol (2,6-DCIP) as a redox indicator. The substrate of acetylthiocholine is hydrolysed by the cholinesterase (ChE) from soil animal pheretima, and the produced thiocholine reacts with the 2,6-DCIP to give obvious shift of electrochemical signal. The inhibition of ChE was assessed by measuring the enzyme activity before and after incubating with parathion-methyl. The reduction peak current of 2,6-DCIP decreases with the time of enzymatical reaction. The ChE loses almost 32.74% activity after 10 min incubation with 1ng mL(-1) paraoxon and 54.62% with 10 microg mL(-1) paraoxon, while the activity that corresponds to 100 microg mL(-1) paraoxon was nearly completely inhibited. This method can be employed to assess the inhibition of ChE and investigate OPs impact on environmental animals.
Qiu
Jingxia
J
Institute of Physical Chemistry, Zhejiang Normal University, Jinhua, China.
Chen
Jin
J
Ma
Qianqian
Q
Miao
Yuqing
Y
D015086:2,6-Dichloroindophenol / Q000737:chemistry*; D000818:Animals; D002138:Calibration; D002800:Cholinesterase Inhibitors / Q000737:chemistry / Q000494:pharmacology; D002802:Cholinesterases / Q000737:chemistry* / Q000378:metabolism; D004563:Electrochemistry / Q000379:methods*; D004566:Electrodes; D009835:Oligochaeta / Q000201:enzymology*; D009943:Organophosphorus Compounds / Q000737:chemistry* / Q000494:pharmacology*; D010084:Oxidation-Reduction; D012989:Soil Pollutants / Q000737:chemistry / Q000494:pharmacology; D013438:Sulfhydryl Compounds / Q000032:analysis / Q000737:chemistry; D013997:Time Factors
D002800:Cholinesterase Inhibitors; D009943:Organophosphorus Compounds; D012989:Soil Pollutants; D013438:Sulfhydryl Compounds; D015086:2,6-Dichloroindophenol; D002802:Cholinesterases