Anti-inflammatory effect of coumarins isolated from Corydalis heterocarpa in HT-29 human colon carcinoma cells.
We investigated anti-inflammatory effects of two coumarins, columbianetin (A) and libanoridin (B), isolated from Corydalis heterocarpa in lipopolysaccharide (LPS)-stimulated HT-29 human colon carcinoma cells. Treatment with compound B inhibited the protein expression levels of inflammatory mediators such as inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), tumor necrosis factor-alpha (TNF-alpha), and interleukin-1 beta (IL-1 beta) in a dose-dependent manner in LPS-stimulated HT-29 cells, but compound A did not. Also, compound B had a higher inhibitory effect on production of cytokines such as IL-1 beta and TNF-alpha in LPS-stimulated HT-29 human colon carcinoma cells than those of compound A. Furthermore, we confirmed that LPS-induced transcription activity of NF-kappaB was inhibited by compound B. As a result of this study, compound B can be considered as a potential anti-inflammatory agent.
Kang
Kyong-Hwa
KH
Department of Chemistry, Pukyong National University, Busan 608-737, Republic of Korea.
Kong
Chang-Suk
CS
Seo
Youngwan
Y
Kim
Moon-Moo
MM
Kim
Se-Kwon
SK
D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000302:isolation & purification* / Q000494:pharmacology*; D000972:Antineoplastic Agents, Phytogenic / Q000302:isolation & purification / Q000494:pharmacology; D015153:Blotting, Western; D002470:Cell Survival / Q000187:drug effects; D004396:Coloring Agents; D031311:Corydalis / Q000737:chemistry*; D003374:Coumarins / Q000302:isolation & purification* / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004797:Enzyme-Linked Immunosorbent Assay; D011564:Furocoumarins / Q000302:isolation & purification* / Q000494:pharmacology*; D019073:HT29 Cells; D006801:Humans; D018836:Inflammation Mediators / Q000032:analysis; D008070:Lipopolysaccharides / Q000494:pharmacology; D009682:Magnetic Resonance Spectroscopy; D021241:Spectrometry, Mass, Electrospray Ionization; D013056:Spectrophotometry, Ultraviolet; D013778:Tetrazolium Salts; D013844:Thiazoles
D000894:Anti-Inflammatory Agents, Non-Steroidal; D000972:Antineoplastic Agents, Phytogenic; D004396:Coloring Agents; D003374:Coumarins; D011564:Furocoumarins; D018836:Inflammation Mediators; D008070:Lipopolysaccharides; D013778:Tetrazolium Salts; D013844:Thiazoles; C544330:libanoridin; C046733:columbianetin; C022616:thiazolyl blue