Analysis of protein-protein interactions by using droplet-based microfluidics.
Every little drop: The K(D) values of angiogenin (ANG) interactions as shown by FRET analysis of thousands of pL-sized droplets agree with data from bulk-fluorescence polarization measurements. Importantly, the use of fluorophores does not affect the activity of ANG or the binding of anti-ANG antibodies to ANG. Such an experimental platform could be applied to the high-throughput analysis of protein-protein interactions.
Srisa-Art
Monpichar
M
Department of Chemistry, Imperial College London, Exhibition Road, London, UK.
Kang
Dong-Ku
DK
Hong
Jongin
J
Park
Hyun
H
Leatherbarrow
Robin J
RJ
Edel
Joshua B
JB
Chang
Soo-Ik
SI
deMello
Andrew J
AJ
D000906:Antibodies / Q000276:immunology; D002478:Cells, Cultured; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods*; D005456:Fluorescent Dyes / Q000737:chemistry; D006801:Humans; D007700:Kinetics; D044085:Microfluidics / Q000295:instrumentation / Q000379:methods*; D025941:Protein Interaction Mapping / Q000379:methods*; D012259:Ribonuclease, Pancreatic / Q000032:analysis* / Q000276:immunology / Q000378:metabolism; D013050:Spectrometry, Fluorescence; D013816:Thermodynamics
D000906:Antibodies; D005456:Fluorescent Dyes; C046366:angiogenin; D012259:Ribonuclease, Pancreatic