Antitumor activity of deoxypodophyllotoxin isolated from Anthriscus sylvestris: Induction of G2/M cell cycle arrest and caspase-dependent apoptosis.
An active compound having antitumor activity was isolated from the root of Anthriscus sylvestris. Structural studies revealed that it was deoxypodophyllotoxin (DPPT), and its biological activity was evaluated in HeLa human cervix carcinoma cells. Flow cytometric analysis showed that DPPT arrests the cell cycle in the G2/M phase prior to apoptosis. The mechanisms of action of DPPT involve inhibition of tubulin polymerization, dysregulation of cyclin A and cyclin B1 expression, and activation of caspases-3 and -7.
Yong
Yeonjoong
Y
Division of Bioscience and Biotechnology, BMIC, RCD, Konkuk University, Seoul 143-701, Republic of Korea.
Shin
Soon Young
SY
Lee
Young Han
YH
Lim
Yoongho
Y
D053148:Caspase 3 / Q000378:metabolism; D053179:Caspase 7 / Q000378:metabolism; D020169:Caspases / Q000378:metabolism*; D002453:Cell Cycle; D002626:Chemistry, Pharmaceutical / Q000379:methods; D019925:Cyclin A / Q000096:biosynthesis; D056744:Cyclin B1 / Q000096:biosynthesis; D004365:Drugs, Chinese Herbal; D005434:Flow Cytometry; D006367:HeLa Cells; D006801:Humans; D008856:Microscopy, Fluorescence / Q000379:methods; D008517:Phytotherapy / Q000379:methods; D010936:Plant Extracts / Q000494:pharmacology*; D018517:Plant Roots / Q000378:metabolism*; D010946:Plants, Medicinal / Q000378:metabolism*; D011034:Podophyllotoxin / Q000031:analogs & derivatives* / Q000032:analysis / Q000494:pharmacology
D019925:Cyclin A; D056744:Cyclin B1; D004365:Drugs, Chinese Herbal; D010936:Plant Extracts; C014451:deoxypodophyllotoxin; D053148:Caspase 3; D053179:Caspase 7; D020169:Caspases; D011034:Podophyllotoxin