title: Stabilizing the urinary activity of fructose-1,6-bisphosphatase with EDTA and mercaptoethanol.
abstract: OBJECTIVE
  The study aim was to establish conditions for stabilization the activity of fructose-1,6-bisphosphatase (FBP-1) in stored urine.
  
  
  METHODS
  The FBP-1 was determined by the method Kepka et. al in a collected fraction of purified urine.
  
  
  RESULTS
  EDTA and mercaptoethanol stabilized FBP-1 activity in stored urine.
  
  
  CONCLUSIONS
  At optimal conditions urine may be stored up to 7 days at a temperature of 4 degrees C.
authors.affiliation: Department of Biochemistry and Experimental Medicine, Children's Memorial Health Institute, Warsaw, Poland.
authors.forename: Alina
authors.initials: A
authors.lastname: Kepka
authors.forename: Sławomir Dariusz
authors.initials: SD
authors.lastname: Szajda
authors.forename: Napoleon
authors.initials: N
authors.lastname: Waszkiewicz
authors.forename: Anna
authors.initials: A
authors.lastname: Zalewska
authors.forename: Paweł
authors.initials: P
authors.lastname: Płudowski
authors.forename: Dorota
authors.initials: D
authors.lastname: Zwierz-Gugała
authors.forename: Beata
authors.initials: B
authors.lastname: Zalewska-Szajda
authors.forename: Małgorzata
authors.initials: M
authors.lastname: Borzym-Kluczyk
authors.forename: Edyta
authors.initials: E
authors.lastname: Kryśkiewicz
authors.forename: Krzysztof
authors.initials: K
authors.lastname: Zwierz
mesh_terms: D002614:Chelating Agents / Q000494:pharmacology; D002648:Child; D003404:Creatinine / Q000652:urine; D004492:Edetic Acid / Q000494:pharmacology*; D004795:Enzyme Stability / Q000187:drug effects; D005260:Female; D006597:Fructose-Bisphosphatase / Q000378:metabolism* / Q000652:urine*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008297:Male; D008623:Mercaptoethanol / Q000494:pharmacology*; D011309:Preservation, Biological / Q000379:methods; D013696:Temperature; D013997:Time Factors
chemical_list: D002614:Chelating Agents; D008623:Mercaptoethanol; D004492:Edetic Acid; D003404:Creatinine; D006597:Fructose-Bisphosphatase