Regulatory role of Sirt1 on the gene expression of fatty acid-binding protein 3 in cultured porcine adipocytes.
To investigate whether Sirt1 could modulate fatty acid-binding protein 3 (FABP3), we treated porcine adipocytes either with the Sirt1 inhibitor nicotinamide (NAM), with the Sirt1 activator resveratrol (RES), or by knockdown of Sirt1 by Sirt1-siRNA. NAM or knockdown with Sirt1-siRNA significantly inhibited Sirt1 mRNA expression, while increasing FABP3 mRNA levels. RES or RES + Sirt1-siRNA treatments further proved that Sirt1 negatively regulated FABP3 gene expression in adipocytes. We also found a similar Sirt1 regulation pattern for PPAR gamma to that of FABP3 in adipocytes. Furthermore, NAM/RES + PPAR gamma-siRNA treatments showed that Sirt1 may regulate the FABP3 gene expression partly through the PPAR gamma-mediated signals. In summary, Sirt1 regulates the expression of FABP3 gene in adipocytes, and PPAR gamma apparently plays an important role in this process.
Shan
Ti Z
TZ
The Key Laboratory of Molecular Animal Nutrition, Ministry of Education, College of Animal Science, Zhejiang University, No. 164 Qiutao North Road, Hangzhou 310029, PR China.
Ren
Yang
Y
Wu
Ting
T
Liu
Chen X
CX
Wang
Yi Z
YZ
D017667:Adipocytes / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D000818:Animals; D002478:Cells, Cultured; D015536:Down-Regulation / Q000187:drug effects; D050556:Fatty Acid-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D005786:Gene Expression Regulation / Q000187:drug effects; D055785:Gene Knockdown Techniques; D009536:Niacinamide / Q000494:pharmacology; D047495:PPAR gamma / Q000235:genetics / Q000378:metabolism; D034741:RNA, Small Interfering / Q000378:metabolism; D000077185:Resveratrol; D037761:Sirtuins / Q000037:antagonists & inhibitors / Q000378:metabolism*; D013267:Stilbenes / Q000494:pharmacology; D034421:Sus scrofa; D013997:Time Factors; D015854:Up-Regulation / Q000187:drug effects
D050556:Fatty Acid-Binding Proteins; D047495:PPAR gamma; D034741:RNA, Small Interfering; D013267:Stilbenes; D009536:Niacinamide; D037761:Sirtuins; D000077185:Resveratrol