title: Numerical chromosome abnormalities are associated with sperm tail swelling patterns.
abstract: OBJECTIVE
  To assess whether the differential sperm tail swelling patterns observed following hypo-osmotic shock are useful in discriminating normal sperm from aneuploid sperm.
  
  
  METHODS
  Prospective study.
  
  
  METHODS
  University research setting.
  
  
  METHODS
  Fluorescence in situ hybridization (FISH) was combined with hypo-osmotic swelling for a simultaneous assessment of aneuploidy and viability in human spermatozoa.
  
  
  METHODS
  FISH for chromosomes 1, 13, 18, 21, X, and Y after hypo-osmotic stress was used to investigate the distribution of sperm aneuploidy related to sperm-tail swelling patterns. A total of 16,473 sperm cells were scored from three normal fertile donors and six oligoasthenoteratozoospermic (OAT) patients.
  
  
  RESULTS
  There was a 17.2-fold decrease in the frequency of total aneuploidy in the sperm with a tail-tip swelling pattern compared with the initial nonselected sperm in the OAT patients. Strikingly, when the sperm with tail-tip swelling patterns were screened from the patients, the frequency of total aneuploidy was actually lower by a factor of four than in the nonselected sperm from fertile donors.
  
  
  CONCLUSIONS
  The sperm cells with tail-tip swelling patterns are related to a low frequency of aneuploidy.
authors.affiliation: Department of Animal Science and Technology and BET Research Institute, Chung-Ang University, Ansung, Gyeonggi-Do, South Korea. mgpang@cau.ac.kr
authors.forename: Myung-Geol
authors.initials: MG
authors.lastname: Pang
authors.forename: Young-Ah
authors.initials: YA
authors.lastname: You
authors.forename: Yoo-Jin
authors.initials: YJ
authors.lastname: Park
authors.forename: Shin-Ae
authors.initials: SA
authors.lastname: Oh
authors.forename: Dai-Sik
authors.initials: DS
authors.lastname: Kim
authors.forename: Young-Ju
authors.initials: YJ
authors.lastname: Kim
mesh_terms: D000327:Adsorption; D000782:Aneuploidy; D016022:Case-Control Studies; D002470:Cell Survival / Q000502:physiology; D002869:Chromosome Aberrations; D025063:Chromosome Disorders / Q000175:diagnosis / Q000235:genetics / Q000473:pathology; D002878:Chromosomes, Human, Pair 1; D002882:Chromosomes, Human, Pair 13; D002891:Chromosomes, Human, Pair 21; D056915:DNA Copy Number Variations / Q000502:physiology; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007248:Infertility, Male / Q000235:genetics; D008297:Male; D009997:Osmotic Pressure / Q000502:physiology*; D013082:Sperm Tail / Q000378:metabolism / Q000473:pathology*; D014867:Water / Q000378:metabolism
chemical_list: D014867:Water