title: Differential effects of zinc and magnesium ions on mineralization activity of phosphatidylserine calcium phosphate complexes.
abstract: Mg(2+) and Zn(2+) are present in the mineral of matrix vesicles (MVs) and biological apatites, and are known to influence the onset and progression of mineral formation by amorphous calcium phosphate (ACP) and hydroxyapatite (HAP). However, neither has been studied systematically for its effect on mineral formation by phosphatidylserine-Ca(2+)-Pi complexes (PS-CPLX), an important constituent of the MV nucleation core. Presented here are studies on the effects of increasing levels of Mg(2+) and Zn(2+) on the process of mineral formation, either when present in synthetic cartilage lymph (SCL), or when incorporated during the formation of PS-CPLX. Pure HAP and PS-CPLX proved to be powerful nucleators, but ACP took much longer to induce mineral formation. In SCL, Mg(2+) and Zn(2+) had significantly different inhibitory effects on the onset and amount of mineral formation; HAP and PS-CPLX were less affected than ACP. Mg(2+) and Zn(2+) caused similar reductions in the rate and length of rapid mineral formation, but Zn(2+) was a more potent inhibitor on a molar basis. When incorporated into PS-CPLX, Mg(2+) and Zn(2+) caused significantly different effects than when present in SCL. Even low, subphysiological levels of Mg(2+) altered the inherent structure of PS-CPLX and markedly reduced its ability to induce and propagate mineral formation. Incorporated Zn(2+) caused significantly less effect, low (<20 microM) levels causing almost no inhibition. Levels of Zn(2+) present in MVs do not appear to inhibit their nucleational activity.
authors.affiliation: Department of Chemistry and Biochemistry, University of South Carolina, Columbia, SC 29208, USA.
authors.forename: Licia N Y
authors.initials: LN
authors.lastname: Wu
authors.forename: Brian R
authors.initials: BR
authors.lastname: Genge
authors.forename: Roy E
authors.initials: RE
authors.lastname: Wuthier
mesh_terms: D002113:Calcification, Physiologic / Q000187:drug effects* / Q000502:physiology; D002130:Calcium Phosphates / Q000138:chemical synthesis / Q000378:metabolism*; D017886:Durapatite / Q000138:chemical synthesis / Q000378:metabolism*; D008274:Magnesium / Q000494:pharmacology*; D010718:Phosphatidylserines / Q000378:metabolism*; D015032:Zinc / Q000494:pharmacology*
chemical_list: D002130:Calcium Phosphates; D010718:Phosphatidylserines; C519480:amorphous calcium phosphate; D017886:Durapatite; D008274:Magnesium; D015032:Zinc