A perspective: metatranscriptomics as a tool for the discovery of novel biocatalysts.
In this mini-review, advantages and current bottlenecks of metatranscriptomic-sequencing for the discovery of novel enzymes for biotechnological applications will be discussed. Comparison of this innovative approach to traditional metagenomic-sequencing will illustrate that expression profiling has a promising future as a direct and very efficient method for the discovery of novel biocatalysts, even from complex microbial communities.
Warnecke
Falk
F
DOE Joint Genome Institute, Microbial Ecology Program, 2800 Mitchell Drive, Walnut Creek, CA 94598, USA. FWarnecke@lbl.gov
Hess
Matthias
M
D001105:Archaea / Q000201:enzymology* / Q000235:genetics; D001419:Bacteria / Q000201:enzymology* / Q000235:genetics; D055162:Biocatalysis; D004798:Enzymes / Q000378:metabolism*; D020869:Gene Expression Profiling; D020745:Genome, Archaeal; D016680:Genome, Bacterial; D023281:Genomics / Q000379:methods*; D017422:Sequence Analysis, DNA
D004798:Enzymes