Infrared radiation influence on molt and regeneration of Neohelice granulata Dana, 1851 (Grapsidae, Sesarminae).
This paper analyzes the influence of infrared radiation (IR) on regeneration, after autotomy of limb buds of Neohelice granulata and consequently the time molt. Eyestalks were ablated to synchronize the start of molt. Afterward, animals were autotomized of five pereopods and divided into control and irradiated groups. The irradiated group was treated for 30 min daily until molt. Limb buds from five animals of days 4, 16 and 20 were collected and histological sections were made from them. These sections were photographed and chitin and epithelium content measured. Another group was made, and after 15 days limb buds were extracted to analyze mitochondrial enzymatic activity from complex I and II. The irradiated group showed a significant reduction in molt time (19.38+/-1.22 days) compared with the control group (32.69+/-1.57 days) and also a significant increase in mitochondrial complex I (388.9+/-27.94%) and II (175.63+/-7.66%) in the irradiated group when compared with the control group (100+/-17.90; 100+/-7.82, respectively). However, these effects were not accompanied by histological alterations in relation to chitin and epithelium. This way, it was possible to demonstrate that IR increases complex I and II activity, reduces the time molt and consequently increases the appendage regeneration rate.
Gonzalez
Vinícius Cunha
VC
Instituto de Ciências Biológicas, Fundação Universidade Federal do Rio Grande (FURG), Rio Grande, Brazil.
Lena
Alexandre Baldez
AB
Espindola
Wendel Aculha
WA
de Souza Votto
Ana Paula
AP
Jorge
Marianna Basso
MB
de Moraes Vaz Batista Filgueira
Daza
D
Bianchini
Adalto
A
Sérgio Varela Júnior
Antônio
A
Nery
Luiz Eduardo Maia
LE
Trindade
Gilma Santos
GS
D000818:Animals; D003445:Crustacea / Q000502:physiology / Q000528:radiation effects*; D007259:Infrared Rays; D018878:Limb Buds / Q000254:growth & development / Q000502:physiology / Q000528:radiation effects; D018978:Molting; D012038:Regeneration