A new strategy to apply Bacillus subtilis MA139 for the production of solid-state fermentation feed.
OBJECTIVE
The study investigated the potential of using Bacillus subtilis MA139 in combination with Lactobacillus fermentum and Saccharomyces cerevisae to produce solid-state fermentation feed.


RESULTS
In a pure fermentation, B. subtilis MA139 was able to grow and synthesize antimicrobial substances at temperatures from 25 to 37 degrees C and at a pH from 5.0 to 9.0. Subsequently, B. subtilis MA139, Lact. fermentum and S. cerevisae were used as starter strains co-inoculated in unsterilized substrate (feed-grade soybean meal and wheat bran). Following 10 days of fermentation in a newly developed plastic bag equipped with a one-way valve, lactic acid bacteria and Bacillus became the predominant strains while S. cerevisae cells decreased slightly. Enterobacteriaceae (Escherichia coli K88 and Salmonella typhimurium) were not detected.


CONCLUSIONS
Use of B. subtilis MA139 as a starter strain co-inoculated with S. cerevisae and Lact. fermentum successfully controlled the growth of enterobacteriaceae.


CONCLUSIONS
This study provided a facile and low-cost way to produce solid-state fermentation feed.
Ying
W
W
State Key Laboratory of Animal Nutrition, China Agricultural University, Beijing, China.
Zhu
R
R
Lu
W
W
Gong
L
L
D000821:Animal Feed / Q000382:microbiology*; D000900:Anti-Bacterial Agents / Q000096:biosynthesis; D001412:Bacillus subtilis / Q000254:growth & development* / Q000378:metabolism*; D004043:Dietary Fiber / Q000378:metabolism / Q000382:microbiology; D004755:Enterobacteriaceae / Q000302:isolation & purification; D005285:Fermentation; D006863:Hydrogen-Ion Concentration; D052197:Limosilactobacillus fermentum / Q000254:growth & development / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000254:growth & development / Q000378:metabolism; D013025:Glycine max / Q000378:metabolism / Q000382:microbiology; D013696:Temperature
D000900:Anti-Bacterial Agents; D004043:Dietary Fiber