Separation and quantitation of Z-isomer in lanoconazole by normal phase HPLC.
An isocratic normal phase high-performance liquid chromatographic (NP-HPLC) method has been developed and validated for the quantitation of Z-isomer in lanoconazole. Separation was achieved with a Thermo Hypersil Silica column. The ratio of 2-propanol, n-hexane and triethylamine in the mobile phase were optimized to obtain the best separation. UV detection was performed at 296 nm. The described method is linear over a range of LOQ--15.0 microg/ml of Z-isomer. The mean recovery of Z-isomer was found to be in the range of 97-99%. The method is simple, rapid, selective, accurate and precise, useful in the quality control of bulk manufacturing.
Kumar
A Phani
AP
Analytical Research, SMS Pharma Research Center, Sanathnagar, Hyderabad, 500 018, India. phani@smspharma.com
Ganesh
V R L
VR
Rao
D V Subba
DV
Anil
C
C
Hariharakrishnan
V S
VS
D000935:Antifungal Agents / Q000032:analysis* / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D007093:Imidazoles / Q000032:analysis* / Q000737:chemistry; D011786:Quality Control; D015203:Reproducibility of Results; D013237:Stereoisomerism
D000935:Antifungal Agents; D007093:Imidazoles; C072774:latoconazole