Spore display using Bacillus thuringiensis exosporium protein InhA.
A new spore display method is presented that enables recombinant proteins to be displayed on the surface of Bacillus spores via fusion with InhA, an exosporium component of Bacillus thuringiensis. The green fluorescent protein and beta-galactosidase as model proteins were fused to the C-terminal region of InhA, respectively. The surface expression of the proteins on the spores was confirmed by flow cytometry, confocal laser scanning microscopy, measurement of the enzyme activity, and an immunogold electron microscopy analysis. InhA-mediated anchoring of foreign proteins in the exosporium of Bacillus spores can provide a new method of microbial display, thereby broadening the potential for novel applications of microbial display.
Park
Tae Jung
TJ
Department of Chemical and Biomolecular Engineering (BK21 Program), BioProcess Engineering Research Center, Center for Systems and Synthetic Biotechnology, and Institute for the BioCentury, KAIST, Daejeon 305-701, Korea.
Choi
Soo-Keun
SK
Jung
Heung-Chae
HC
Lee
Sang Yup
SY
Pan
Jae-Gu
JG
D000954:Antigens, Surface / Q000378:metabolism; D001413:Bacillus thuringiensis / Q000378:metabolism* / Q000648:ultrastructure; D001426:Bacterial Proteins / Q000378:metabolism*; D005434:Flow Cytometry; D017930:Genes, Reporter; D049452:Green Fluorescent Proteins; D007150:Immunohistochemistry; D018613:Microscopy, Confocal; D046529:Microscopy, Electron, Transmission; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000235:genetics; D013171:Spores, Bacterial / Q000378:metabolism* / Q000648:ultrastructure
D000954:Antigens, Surface; D001426:Bacterial Proteins; D011993:Recombinant Fusion Proteins; D049452:Green Fluorescent Proteins