Prion strain discrimination based on rapid in vivo amplification and analysis by the cell panel assay.
Prion strain identification has been hitherto achieved using time-consuming incubation time determinations in one or more mouse lines and elaborate neuropathological assessment. In the present work, we make a detailed study of the properties of PrP-overproducing Tga20 mice. We show that in these mice the four prion strains examined are rapidly and faithfully amplified and can subsequently be discriminated by a cell-based procedure, the Cell Panel Assay.
Karapetyan
Yervand Eduard
YE
Department of Infectology, The Scripps Research Institute, Scripps Florida, Jupiter, Florida, United States of America.
Saá
Paula
P
Mahal
Sukhvir Paul
SP
Sferrazza
Gian Franco
GF
Sherman
Alexandra
A
Salès
Nicole
N
Weissmann
Charles
C
Lasmézas
Corinne Ida
CI
D000818:Animals; D001681:Biological Assay / Q000379:methods*; D001921:Brain / Q000378:metabolism / Q000473:pathology; D051379:Mice; D008810:Mice, Inbred C57BL; D018621:PrPC Proteins / Q000378:metabolism; D018620:PrPSc Proteins / Q000378:metabolism; D011328:Prions / Q000145:classification* / Q000378:metabolism* / Q000472:pathogenicity; D013045:Species Specificity
D018621:PrPC Proteins; D018620:PrPSc Proteins; D011328:Prions