Arabidopsis radical-induced cell death1 is involved in UV-B signaling.
The Arabidopsis radical-induced cell death1 (rcd1) mutant is sensitive to ozone fumigation and apoplastic superoxide, but tolerant to methyl viologen. In the present article, we report that the rcd1 mutant is also tolerant to supplementary UV-B radiation. The rcd1-1 mutant exhibits less accumulation of TT dimers, increased hypocotyl growth inhibition and higher accumulation of flavonoids under supplemental UV-B radiation. Moreover, the expression of HY5 (elongated hypocotyl5) is increased in the mutant after UV-B treatment. Gene expression downstream of UV-B signaling reveals that COP1 (constitutively photomorphogenic1)-regulated genes have an elevated expression in rcd1-1 mutant under UV-B radiation, while expression of UVR8 (UV resistance locus 8)-regulated and HY5-independent genes are not changed. Interestingly, the expression of RCD1 genes is not significantly changed by UV-B radiation. Previous study has shown that STO protein is interacting with RCD1 in vitro. Here, we found the mRNA level of STO (salt tolerance) is greatly increased in rcd1-1 mutant after UV-B radiation. However, UV-B-induced HY5 and CHS expression is partially inhibited in sto mutant. Based on the above results, it is deduced that the RCD1, working together with STO, is involved in Arabidopsis UV-B signaling.
Jiang
Lei
L
Key Laboratory of Ecology and Environmental Science in Guangdong Higher Education, School of Life Science, South China Normal University, Guangzhou 510631, China.
Wang
Yan
Y
Björn
Lars Olof
LO
Li
Shaoshan
S
D017360:Arabidopsis / Q000235:genetics / Q000254:growth & development / Q000378:metabolism* / Q000528:radiation effects*; D029681:Arabidopsis Proteins / Q000235:genetics* / Q000378:metabolism*; D050976:Basic-Leucine Zipper Transcription Factors / Q000235:genetics; D004249:DNA Damage / Q000235:genetics; D004260:DNA Repair / Q000235:genetics; D003624:Darkness; D018506:Gene Expression Regulation, Plant / Q000528:radiation effects; D018546:Hypocotyl / Q000235:genetics / Q000254:growth & development / Q000528:radiation effects; D009154:Mutation; D009687:Nuclear Proteins / Q000235:genetics* / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011836:Radiation Tolerance / Q000235:genetics; D015398:Signal Transduction / Q000235:genetics / Q000528:radiation effects*; D044767:Ubiquitin-Protein Ligases / Q000235:genetics; D014466:Ultraviolet Rays / Q000009:adverse effects
D029681:Arabidopsis Proteins; D050976:Basic-Leucine Zipper Transcription Factors; C109254:HY5 protein, Arabidopsis; D009687:Nuclear Proteins; C492758:RCD1 protein, Arabidopsis; D012333:RNA, Messenger; C100336:STO protein, Arabidopsis; C471983:AT2G32950 protein, Arabidopsis; D044767:Ubiquitin-Protein Ligases