The importance of starch and sucrose digestion in nutritive biology of synanthropic acaridid mites: alpha-amylases and alpha-glucosidases are suitable targets for inhibitor-based strategies of mite control.
The adaptation of nine species of mites that infest stored products for starch utilization was tested by (1) enzymatic analysis using feces and whole mite extracts, (2) biotests, and (3) inhibition experiments. Acarus siro, Aleuroglyphus ovatus, and Tyroborus lini were associated with the starch-type substrates and maltose, with higher enzymatic activities observed in whole mite extracts. Lepidoglyphus destructor was associated with the same substrates but had higher activities in feces. Dermatophagoides farinae, Chortoglyphus arcuatus, and Caloglyphus redickorzevi were associated with sucrose. Tyrophagus putrescentiae and Carpoglyphus lactis had low or intermediate enzymatic activity on the tested substrates. Biotests on starch additive diets showed accelerated growth of species associated with the starch-type substrates. The inhibitor acarbose suppressed starch hydrolysis and growth of the mites. We suggest that the species with higher starch hydrolytic activity in feces were more tolerant to acarbose, and alpha-amylase and alpha-glucosidase of synanthropic mites are suitable targets for inhibitor-based strategies of mite control.
Erban
Tomas
T
Crop Research Institute, Prague, Czech Republic. arachnid@centrum.cz
Erbanova
Michaela
M
Nesvorna
Marta
M
Hubert
Jan
J
D040041:Acaridae / Q000145:classification / Q000502:physiology*; D000818:Animals; D004063:Digestion; D004791:Enzyme Inhibitors / Q000494:pharmacology; D065089:Glycoside Hydrolase Inhibitors; D006868:Hydrolysis; D008320:Maltose / Q000378:metabolism; D010571:Pest Control / Q000379:methods*; D013045:Species Specificity; D013213:Starch / Q000378:metabolism*; D013395:Sucrose / Q000378:metabolism*; D000516:alpha-Amylases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D000520:alpha-Glucosidases / Q000378:metabolism*
D004791:Enzyme Inhibitors; D065089:Glycoside Hydrolase Inhibitors; D013395:Sucrose; D008320:Maltose; D013213:Starch; D000516:alpha-Amylases; D000520:alpha-Glucosidases