title: Bone formation ability of carbonate apatite-collagen scaffolds with different carbonate contents.
abstract: Hydroxyapatite and carbonate apatites with different carbonate contents were synthesized, mixed with atelocollagen, and made into sponge scaffolds. The scaffolds were implanted into the bone sockets of the femurs of male New Zealand white rabbits for 2, 3, 12 and 24 weeks. carbonate apatite-collagen scaffold with 4.8 wt% carbonate content appeared to have similar crystallinity and chemical composition to human bone. When the scaffolds were implanted into the rabbit femurs, histological observation indicated that the carbonate apatites-collagen scaffolds with relatively higher carbonate contents were gradually deformed throughout the implantation period, and showed uniform surrounding bone after 24 weeks and could not be distinguished. The carbonate apatite-collagen scaffold with 4.8 wt% carbonate content showed the highest bone area ratio of all of the scaffolds. It is suggested that a carbonate apatite-collagen scaffold with carbonate content similar to that of human bone may have optimal bone formation ability.
authors.affiliation: Department of Advanced Prosthodontics, Graduate School of Biomedical Sciences, Hiroshima University, 1-2-3 Kasumi, Minami-ku, Hiroshima 734-8553, Japan.
authors.forename: Ayumu
authors.initials: A
authors.lastname: Matsuura
authors.forename: Takayasu
authors.initials: T
authors.lastname: Kubo
authors.forename: Kazuya
authors.initials: K
authors.lastname: Doi
authors.forename: Kazuhiko
authors.initials: K
authors.lastname: Hayashi
authors.forename: Kouji
authors.initials: K
authors.lastname: Morita
authors.forename: Rie
authors.initials: R
authors.lastname: Yokota
authors.forename: Hidetaka
authors.initials: H
authors.lastname: Hayashi
authors.forename: Isao
authors.initials: I
authors.lastname: Hirata
authors.forename: Masayuki
authors.initials: M
authors.lastname: Okazaki
authors.forename: Yasumasa
authors.initials: Y
authors.lastname: Akagawa
mesh_terms: D000818:Animals; D001031:Apatites / Q000032:analysis / Q000737:chemistry*; D001672:Biocompatible Materials / Q000032:analysis / Q000737:chemistry*; D001847:Bone Diseases / Q000473:pathology / Q000601:surgery; D018786:Bone Substitutes / Q000737:chemistry; D002118:Calcium / Q000032:analysis; D002254:Carbonates / Q000032:analysis; D003094:Collagen / Q000737:chemistry*; D017886:Durapatite / Q000737:chemistry; D005269:Femur / Q000473:pathology / Q000601:surgery; D008297:Male; D008855:Microscopy, Electron, Scanning; D010011:Osteocytes / Q000473:pathology; D010012:Osteogenesis / Q000502:physiology*; D010758:Phosphorus / Q000032:analysis; D011817:Rabbits; D017550:Spectroscopy, Fourier Transform Infrared; D013997:Time Factors; D054457:Tissue Scaffolds / Q000737:chemistry*; D014961:X-Ray Diffraction; D055114:X-Ray Microtomography
chemical_list: D001031:Apatites; D001672:Biocompatible Materials; D018786:Bone Substitutes; D002254:Carbonates; C059821:atelocollagen; D010758:Phosphorus; C030782:carboapatite; D003094:Collagen; D017886:Durapatite; D002118:Calcium