title: First report of natural infection in cats with Leishmania infantum in Iran.
abstract: Visceral leishmaniasis (VL), caused by Leishmania infantum, is an endemic zoonosis in Iran. In recent years, Leishmania infection in cats has been reported in several countries where leishmaniasis is present. The aim of this study was to survey Leishmania infection in cats and to detect its causative agents in VL endemic areas in Iran. Forty stray cats were captured from two areas where VL is endemic, Fars and East Azerbaijan provinces. Infection with Leishmania was evaluated by parasitological and molecular methods. Leishmania parasites were detected in 4 out of 40 cats (10%). The parasite was isolated from the spleen of three and the liver of one cat and mass cultivated in the culture medium. Molecular and isoenzyme characterization revealed that the parasites isolated from the four cats were all L. infantum. Considering the high rate of feline Leishmania infection in this study, it may be suggested that cats have an epidemiological role in areas where VL is endemic in Iran.
authors.affiliation: Department of Parasitology and Mycology, Parasitology and Mycology Research Center, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
authors.forename: Gholam Reza
authors.initials: GR
authors.lastname: Hatam
authors.forename: Seyed Jafar
authors.initials: SJ
authors.lastname: Adnani
authors.forename: Qasem
authors.initials: Q
authors.lastname: Asgari
authors.forename: Esmael
authors.initials: E
authors.lastname: Fallah
authors.forename: Mohammad Hossein
authors.initials: MH
authors.lastname: Motazedian
authors.forename: Seyed Mahmoud
authors.initials: SM
authors.lastname: Sadjjadi
authors.forename: Bahador
authors.initials: B
authors.lastname: Sarkari
mesh_terms: D000818:Animals; D002371:Cat Diseases / Q000453:epidemiology / Q000469:parasitology*; D002415:Cats; D005260:Female; D007492:Iran / Q000453:epidemiology; D007527:Isoenzymes / Q000378:metabolism; D018314:Leishmania infantum / Q000302:isolation & purification*; D007898:Leishmaniasis, Visceral / Q000453:epidemiology / Q000469:parasitology / Q000662:veterinary*; D008099:Liver / Q000469:parasitology; D008297:Male; D016133:Polymerase Chain Reaction; D013154:Spleen / Q000469:parasitology
chemical_list: D007527:Isoenzymes