title: Expression of beta-catenin and its mechanism of delocalization in intestinal-type early gastric cancer based on mucin expression.
abstract: The biological characteristics of intestinal-type early gastric cancers (ICs) differ based on mucin phenotypes. Beta-catenin delocalization is a predictive marker of aggressive biological behavior (submucosal invasion and lymph node metastasis) of ICs. The presumptive causative genetic alterations leading to delocalization of beta-catenin in ICs are still controversial, and there are only a few reports regarding beta-catenin expression in gastric cancer based on mucin phenotypes. Therefore, in the current study, the expression and mechanisms of delocalization of beta-catenin were elucidated on the basis of mucin phenotypes in 109 cases of ICs. There was increased cytoplasmic and nuclear beta-catenin expression (delocalization) in ICs with a predominant intestinal mucin phenotype (ICIP; 46.3% [25/54 cases]) compared to ICs with a predominant gastric mucin phenotype (ICGP; 20% [11/55 cases]). There were no beta-catenin or APC mutations in ICs. APC promoter hypermethylation was present in 49 of 105 (46.7%) cases of ICs. There was a significant relationship between APC promoter hypermethylation and beta-catenin delocalization in ICs, especially in ICIPs. There was no relationship between beta-catenin delocalization and APC gene loss of heterozygosity in ICs. In conclusion, we showed that beta-catenin delocalization was more evident in ICIPs, and APC promoter hypermethylation might play a role in delocalization of beta-catenin, especially in ICIPs.
authors.affiliation: Department of Pathology, Pusan National University School of Medicine, Busan, Republic of Korea.
authors.forename: Soo Han
authors.initials: SH
authors.lastname: Lee
authors.forename: Hyun Jeong
authors.initials: HJ
authors.lastname: Kang
authors.forename: Dong-Hun
authors.initials: DH
authors.lastname: Shin
authors.forename: Duk-Yeon
authors.initials: DY
authors.lastname: Cho
authors.forename: Jin Mi
authors.initials: JM
authors.lastname: Song
authors.forename: Han Cheol
authors.initials: HC
authors.lastname: Lee
authors.forename: Gwang Ha
authors.initials: GH
authors.lastname: Kim
authors.forename: Geun Am
authors.initials: GA
authors.lastname: Song
authors.forename: Mee Young
authors.initials: MY
authors.lastname: Sol
authors.forename: Jee Yeon
authors.initials: JY
authors.lastname: Kim
authors.forename: Kyung Un
authors.initials: KU
authors.lastname: Choi
authors.forename: Chang Hun
authors.initials: CH
authors.lastname: Lee
authors.forename: Gi Young
authors.initials: GY
authors.lastname: Huh
authors.forename: Do Youn
authors.initials: DY
authors.lastname: Park
mesh_terms: D000328:Adult; D000368:Aged; D000483:Alleles; D001483:Base Sequence; D002467:Cell Nucleus / Q000378:metabolism; D019175:DNA Methylation; D004252:DNA Mutational Analysis; D004273:DNA, Neoplasm / Q000235:genetics / Q000302:isolation & purification; D005260:Female; D005743:Gastrectomy; D005753:Gastric Mucosa / Q000378:metabolism / Q000473:pathology; D015972:Gene Expression Regulation, Neoplastic; D017491:Genes, APC; D006801:Humans; D007150:Immunohistochemistry; D019656:Loss of Heterozygosity; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D009077:Mucins / Q000378:metabolism*; D009154:Mutation; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D018807:Polymorphism, Single-Stranded Conformational; D011401:Promoter Regions, Genetic; D012189:Retrospective Studies; D017422:Sequence Analysis, DNA; D013274:Stomach Neoplasms / Q000235:genetics* / Q000378:metabolism* / Q000473:pathology; D013997:Time Factors; D051176:beta Catenin / Q000235:genetics / Q000378:metabolism*
chemical_list: C495270:CTNNB1 protein, human; D004273:DNA, Neoplasm; D009077:Mucins; D051176:beta Catenin