Isolation and characterization of a novel lysine racemase from a soil metagenomic library.
A lysine racemase (lyr) gene was isolated from a soil metagenome by functional complementation for the first time by using Escherichia coli BCRC 51734 cells as the host and d-lysine as the selection agent. The lyr gene consisted of a 1,182-bp nucleotide sequence encoding a protein of 393 amino acids with a molecular mass of about 42.7 kDa. The enzyme exhibited higher specific activity toward lysine in the l-lysine-to-d-lysine direction than in the reverse reaction.
Chen
I-Chien
IC
Institute of Molecular Biology, College of Life Sciences, [corrected] National Chung Hsing University, Taichung, Taiwan.
Lin
Wei-De
WD
Hsu
Shin-Kuang
SK
Thiruvengadam
Venkatesan
V
Hsu
Wen-Hwei
WH
D000591:Amino Acid Isomerases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D000595:Amino Acid Sequence; D001483:Base Sequence; D002413:Cations, Divalent / Q000494:pharmacology; D003001:Cloning, Molecular; D003067:Coenzymes / Q000494:pharmacology; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D015723:Gene Library; D005816:Genetic Complementation Test; D008239:Lysine / Q000378:metabolism*; D008969:Molecular Sequence Data; D008970:Molecular Weight; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology
D002413:Cations, Divalent; D003067:Coenzymes; D004269:DNA, Bacterial; D000591:Amino Acid Isomerases; C084211:lysine racemase; D008239:Lysine