title: Crosslinking heparin to collagen scaffolds for the delivery of human platelet-derived growth factor.
abstract: Platelet-derived growth factor (PDGF) plays an important role in tissue regeneration and wound repair. However, the lack of effective delivery and the efficient targeting specificity limits its clinical applications. Here, heparin possessing PDGF binding domain was crosslinked to the collagen-based demineralized bone matrix (DBM) for the delivery of human PDGF(HC-PDGF). In in vitro experiments, heparin improves the binding of PDGF to collagen. In vitro activity assay indicates that collagen-heparin-PDGF (CH-PDGF) promotes human fibroblasts to proliferate on collagen gel. In addition, HC-PDGF stimulates cells to migrate into DBM scaffolds after implantation. The histological analysis shows that HC-PDGF promotes vascularization of the implants. In summary, heparin-DBM/PDGF could prevent the diffusion of PDGF, prolong its activity, and promote the cellularization and vascularization of the scaffold.
authors.affiliation: College of Life Sciences, Shandong University, Jinan 250100, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Sun
authors.forename: Bing
authors.initials: B
authors.lastname: Chen
authors.forename: Yannan
authors.initials: Y
authors.lastname: Zhao
authors.forename: Wenjie
authors.initials: W
authors.lastname: Sun
authors.forename: Kaoshan
authors.initials: K
authors.lastname: Chen
authors.forename: Jing
authors.initials: J
authors.lastname: Zhang
authors.forename: Zhanliang
authors.initials: Z
authors.lastname: Wei
authors.forename: Zhifeng
authors.initials: Z
authors.lastname: Xiao
authors.forename: Jianwu
authors.initials: J
authors.lastname: Dai
mesh_terms: D003094:Collagen / Q000737:chemistry*; D003432:Cross-Linking Reagents / Q000737:chemistry*; D004337:Drug Carriers / Q000737:chemistry*; D016503:Drug Delivery Systems; D048091:Guided Tissue Regeneration / Q000379:methods; D006493:Heparin / Q000737:chemistry*; D006801:Humans; D010982:Platelet-Derived Growth Factor / Q000737:chemistry / Q000378:metabolism*; D054457:Tissue Scaffolds; D014945:Wound Healing
chemical_list: D003432:Cross-Linking Reagents; D004337:Drug Carriers; D010982:Platelet-Derived Growth Factor; D006493:Heparin; D003094:Collagen