The combined effect of mineral trioxide aggregate and enamel matrix derivative on odontoblastic differentiation in human dental pulp cells.
BACKGROUND
The current study investigated the effectiveness of enamel matrix derivative (EMD) and mineral trioxide aggregate (MTA) in inducing the differentiation of human dental pulp cells (HDPCs) into odontoblast-like cells in vitro.


METHODS
The effects of MTA and EMD on odontoblastic differentiation were indexed by alkaline phosphatase (ALP) activity and the expression of odontoblastic/osteoblastic markers, as determined by reverse-transcription polymerase chain reaction analysis. Mineralization was also evaluated by the staining of calcium deposits with Alizarin red. Cells were treated with a combination of MTA and EMD or with MTA alone.


RESULTS
Compared with MTA-treated cells on day 3, MTA/EMD-treated cells exhibited significantly greater increases in ALP activity and in dentin sialophosphoprotein and bone sialoprotein expression. Mineralization was significantly greater on day 7 in MTA/EMD-treated cells than in MTA-treated cells.


CONCLUSIONS
These results suggest that a combination of MTA and EMD promotes more rapid differentiation in HDPCs than MTA alone and that this combination is thus a potential pulp-capping agent.
Min
Kyung-San
KS
Department of Conservative Dentistry, School of Dentistry, Wonkwang University, Iksan, South Korea.
Yang
Seong-Hak
SH
Kim
Eun-Cheol
EC
D017607:Aluminum Compounds / Q000494:pharmacology*; D002113:Calcification, Physiologic / Q000187:drug effects; D017610:Calcium Compounds / Q000494:pharmacology*; D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D003746:Dental Enamel Proteins / Q000494:pharmacology*; D003782:Dental Pulp / Q000166:cytology / Q000187:drug effects*; D003809:Dentin, Secondary / Q000254:growth & development / Q000378:metabolism; D004338:Drug Combinations; D004357:Drug Synergism; D016326:Extracellular Matrix Proteins / Q000096:biosynthesis; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D058574:Integrin-Binding Sialoprotein; D009804:Odontoblasts / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D010087:Oxides / Q000494:pharmacology*; D010750:Phosphoproteins; D012333:RNA, Messenger / Q000096:biosynthesis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012387:Root Canal Filling Materials / Q000494:pharmacology*; D012795:Sialoglycoproteins / Q000096:biosynthesis; D017640:Silicates / Q000494:pharmacology*; D000099311:Dentin Sialophosphoprotein
D017607:Aluminum Compounds; D017610:Calcium Compounds; D003746:Dental Enamel Proteins; D004338:Drug Combinations; D016326:Extracellular Matrix Proteins; D058574:Integrin-Binding Sialoprotein; D010087:Oxides; D010750:Phosphoproteins; D012333:RNA, Messenger; D012387:Root Canal Filling Materials; D012795:Sialoglycoproteins; D017640:Silicates; D000099311:Dentin Sialophosphoprotein; C546871:IBSP protein, human; C109069:enamel matrix proteins; C086631:mineral trioxide aggregate