Competitive RT-PCR Strategy for Quantitative Evaluation of the Expression of Tilapia (Oreochromis niloticus) Growth Hormone Receptor Type I.
Quantization of gene expression requires that an accurate measurement of a specific transcript is made. In this paper, a quantitative reverse transcription-polymerase chain reaction (RT-PCR) by competition for tilapia growth hormone receptor type I is designed and validated. This experimental procedure was used to determine the abundance of growth hormone receptor type I transcript in different tilapia tissues. The results obtained with this developed competitive RT-PCR were similar to real-time PCR results reported recently. This protocol provides a reliable alternative, but less expensive than real-time PCR to quantify specific genes.


BACKGROUND
The online version of this article (DOI:10.1007/s12575-009-9002-3) contains supplementary material, which is available to authorized users.
Rodríguez-Mallon
Alina
A
Aquatic Biotechnology Department, Animal Biotechnology Division, Center for Genetic Engineering and Biotechnology, P.O. Box 6162, Havana, 10 600 Cuba.
Cárdenas
Yamilet
Y
Lugo
Juana María
JM
Oliva
Aymé
A
Morales
Antonio
A
Estrada
Mario Pablo
MP