[Effects of Batroxobin on distal anastomotic intimal hyperplasia after expanded polytetrafluoroethylene bypass grafting in dog common carotid artery].
OBJECTIVE
To study the morphological behavior, the expression of MCP-1 and PDGF-B of distal anastomotic neointima after expanded polytetrafluoroethylene (ePTFE) bypass grafting. And to identify the effects of Batroxobin (BX) on distal anastomotic intimal hyperplasia.


METHODS
12 adult mongrel dogs underwent end to side bypass grafting in left common carotid artery using ePTFE vascular graft (6mm in diameter, 5cm in length) were divided randomly into two groups. All 12 bypass grafts were removed 28 days after grafting and distal anastomotic parts were obtained. Using HE, Masson, IHC, RT-PCR and western blot, we analyzed the thickness, relative content of ECM, SMC PCNA index, expression of MCP-1 and PDGF-B in distal anastomotic neointima. And we compared the differences between the two groups.


RESULTS
28 day after grafting, the neointima was formed by SMC and ECM and was covered by epithelial cells. After using BX, the thickness of neointima was reduced [( 381.3 +/- 144.7) microm versus (213.8 +/- 29.0) microm, P < 0.05] and the relative content of ECM were significantly decreased (P = 0.006). The PCNA index (P = 0.109) and PDGF-B level (P = 0.055 by RT-PCR and P = 0.337 by WB) between the two groups had no significant difference. MCP-1 level was markedly decreased (P = 0.025 by RT-PCR and P = 0.016 by WB).


CONCLUSIONS
Using BX after ePTFE grafting could effectively reduce the relative content of ECM, reduce the expression of MCP-1 and decrease the thickness of neointima in the early time.
Chen
Ye-Ye
YY
Department of Vascular Surgery, Peking Union Medical College Hospital, Chinese Academy of Medical Science and Peking Union Medical College, Beijing 100730, China.
Liu
Chang-Wei
CW
Ye
Wei
W
Zhang
Ran
R
Wei
Yu-Sheng
YS
Liu
De-Pei
DP
D000714:Anastomosis, Surgical; D000818:Animals; D012103:Batroxobin / Q000494:pharmacology*; D001807:Blood Vessel Prosthesis; D017536:Carotid Artery, Common / Q000601:surgery*; D018932:Chemokine CCL2 / Q000378:metabolism; D004285:Dogs; D005260:Female; D006965:Hyperplasia; D008297:Male; D011138:Polytetrafluoroethylene; D020574:Proto-Oncogene Proteins c-sis / Q000378:metabolism; D054047:Surgical Stomas / Q000473:pathology*; D017539:Tunica Intima / Q000187:drug effects* / Q000473:pathology*; D014656:Vascular Surgical Procedures
D018932:Chemokine CCL2; D020574:Proto-Oncogene Proteins c-sis; D011138:Polytetrafluoroethylene; D012103:Batroxobin