[Study on apoptosis of human stomach SGC-7901 cells induced by extracts of Solanum lyratum].
OBJECTIVE
To investigate the effects of extracts of Solanum lyratum (ESL) on the apoptosis of Human stomach cancer SGC-7901 cells.


METHODS
Dried whole herbs of Solanum lyratum were extracted by boiling distilled water. SGC-7901 cells were randomly divided into control group, ESL-treated groups (12.5 g/L, 25 g/L, 50 g/L) and the positive control (25 mg/L DDP) group. The growth inhibitory rate was evaluated by MTT assay. Morphological changes of apoptosis were observed with fluorescence microscope. Cell apoptosis rate was determined by flow cytometry. Expressions of bcl-xl, Caspase-9 and bid mRNA were detected by semi-quantitive RT-PCR. The activity of Caspase-3 was detected by Fluorospectrophotometry.


RESULTS
Compared with control group, the cell proliferation inhibitory rate and apoptosis rate of human stomach cancer SGC-7901 cells increased obviously (P < 0.05). There were obvious changes of morphology of the SGC-7901 cells as the nuclear shrinkage, chromatin condensation and margination; The expression of bcl-xl mRNA decreased obviously (P < 0.05), the expression of Caspase-9 and bid mRNA increased obviously respectively (P < 0.05), and displayed effect in a dose-dependent manner in the SGC-7901 cells of the ESL-treated groups. The activity of Caspase-3 in the SGC-7901 cells of the ESL-treated groups were higher than that of the control group significantly (P < 0.01).


CONCLUSIONS
ESL can induce apoptosis and inhibit proliferation of the human stomach cancer SGC-7901 cells by regulating expression of bcl-xl, Caspase-9 and bid genes and strengthening the activity of Caspase-3.
Wan
Fu-sheng
FS
Department of Biochemistry and Molecular Biology, Medical College of Nanchang University, Nanchang 330006, China. wanfs01@163.com
Wu
Jian
J
Li
Hua
H
Tu
Shuo
S
Yu
Le-han
LH
D000972:Antineoplastic Agents, Phytogenic / Q000302:isolation & purification / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D020169:Caspases / Q000235:genetics / Q000378:metabolism; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002945:Cisplatin / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004365:Drugs, Chinese Herbal / Q000302:isolation & purification / Q000494:pharmacology*; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D006801:Humans; D010946:Plants, Medicinal / Q000737:chemistry; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D032322:Solanum / Q000737:chemistry*; D013274:Stomach Neoplasms / Q000378:metabolism / Q000473:pathology*; D051020:bcl-X Protein / Q000378:metabolism
D000972:Antineoplastic Agents, Phytogenic; D004365:Drugs, Chinese Herbal; D012333:RNA, Messenger; D051020:bcl-X Protein; D020169:Caspases; D002945:Cisplatin