title: Prion strain discrimination based on rapid in vivo amplification and analysis by the cell panel assay.
abstract: Prion strain identification has been hitherto achieved using time-consuming incubation time determinations in one or more mouse lines and elaborate neuropathological assessment. In the present work, we make a detailed study of the properties of PrP-overproducing Tga20 mice. We show that in these mice the four prion strains examined are rapidly and faithfully amplified and can subsequently be discriminated by a cell-based procedure, the Cell Panel Assay.
authors.affiliation: Department of Infectology, The Scripps Research Institute, Scripps Florida, Jupiter, Florida, United States of America.
authors.forename: Yervand Eduard
authors.initials: YE
authors.lastname: Karapetyan
authors.forename: Paula
authors.initials: P
authors.lastname: Saá
authors.forename: Sukhvir Paul
authors.initials: SP
authors.lastname: Mahal
authors.forename: Gian Franco
authors.initials: GF
authors.lastname: Sferrazza
authors.forename: Alexandra
authors.initials: A
authors.lastname: Sherman
authors.forename: Nicole
authors.initials: N
authors.lastname: Salès
authors.forename: Charles
authors.initials: C
authors.lastname: Weissmann
authors.forename: Corinne Ida
authors.initials: CI
authors.lastname: Lasmézas
mesh_terms: D000818:Animals; D001681:Biological Assay / Q000379:methods*; D001921:Brain / Q000378:metabolism / Q000473:pathology; D051379:Mice; D008810:Mice, Inbred C57BL; D018621:PrPC Proteins / Q000378:metabolism; D018620:PrPSc Proteins / Q000378:metabolism; D011328:Prions / Q000145:classification* / Q000378:metabolism* / Q000472:pathogenicity; D013045:Species Specificity
chemical_list: D018621:PrPC Proteins; D018620:PrPSc Proteins; D011328:Prions