[Association study on the mitochondrial genome region np16181-16193 variation with type 2 diabetes mellitus].
OBJECTIVE
To investigate the association of the mitochondrial DNA region np16181-16193 variations with type 2 diabetes mellitus (T2DM).


METHODS
Blood samples of 199 unrelated T2DM patients and 205 normal controls were collected to detect the mitochondrial DNA region np16181-16193 variations by PCR and sequencing, and to analyze the association of the variations with the major clinical symptoms.


RESULTS
The mitochondrial DNA np16181-16193 region is a hypervariable area, with several polymorphisms. Four types of np16181-16193 region variations were found only in T2DM. The 1-hour postprandial blood glucose (P1BG) in the T2DM individuals with np16181-16193 region variations was significantly higher than those without variations (P<0.05), while there was no significant difference in other biochemical parameters (P>0.05).


CONCLUSIONS
The mitochondrial DNA np16181-16193 variations could not be regarded as a risk factor for T2DM.
Chen
Fang-jian
FJ
Zhejiang Provincial Key Laboratory of Medical Genetics, Wenzhou Medical College, Wenzhou, Zhejiang, 325035 PR China.
Yu
Hong
H
Lin
Hong
H
Hu
Chao-hui
CH
Hu
Ya-guo
YG
Lv
Jian-xin
JX
D000328:Adult; D022801:Complementarity Determining Regions / Q000235:genetics*; D004252:DNA Mutational Analysis; D004272:DNA, Mitochondrial / Q000032:analysis*; D003924:Diabetes Mellitus, Type 2 / Q000235:genetics*; D005260:Female; D020022:Genetic Predisposition to Disease; D054629:Genome, Mitochondrial / Q000235:genetics*; D006801:Humans; D008297:Male; D017422:Sequence Analysis, DNA
D022801:Complementarity Determining Regions; D004272:DNA, Mitochondrial