Effect of melatonin on burn-induced gastric mucosal injury in rats.
We studied the effect of melatonin treatment on gastric mucosal damage induced by experimental burns and its possible relation to changes in gastric lipid peroxidation status. Melatonin was intraperitoneally applied immediately after third-degree burns over 30% of total body skin surface area of rats. Malondialdehyde (MDA), uric acid (UA) and sulphydril (SH) levels were determined in gastric mucosa and blood plasma and used as biomarkers of the oxidative stress. The results showed that the skin burn caused oxidative stress evidenced by accumulation of MDA and UA as well as the depletion of SHs in gastric mucosa. Plasma MDA concentrations were elevated, while plasma SH concentrations were decreased after burns. Melatonin (10 mg per kg body weight) protected gastric mucosa from oxidative damage by suppressing lipid peroxidation and activating the antioxidant defence. It may be hypothesised that melatonin restores the redox balance in the gastric mucosa and protects it from burn-induced oxidative injury. Melatonin has no significant influence on the concentrations of plasma MDA and antioxidants after burn; therefore, it should largely be considered as a limiting factor for tissue-damage.
Bekyarova
G
G
Department of Pathophysiology, Medical University of Varna, 55 Marin Drinov Street, Varna 9002, Bulgaria. gabekyarova2001@yahoo.com
Galunska
B
B
Ivanova
D
D
Yankova
T
T
D000818:Animals; D000975:Antioxidants / Q000627:therapeutic use*; D015415:Biomarkers / Q000097:blood / Q000378:metabolism; D002056:Burns / Q000188:drug therapy* / Q000378:metabolism; D004353:Drug Evaluation, Preclinical / Q000379:methods; D005753:Gastric Mucosa / Q000187:drug effects / Q000293:injuries* / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D008315:Malondialdehyde / Q000097:blood / Q000378:metabolism; D008550:Melatonin / Q000627:therapeutic use*; D051381:Rats; D013438:Sulfhydryl Compounds / Q000097:blood / Q000378:metabolism; D014527:Uric Acid / Q000097:blood / Q000378:metabolism
D000975:Antioxidants; D015415:Biomarkers; D013438:Sulfhydryl Compounds; D014527:Uric Acid; D008315:Malondialdehyde; D008550:Melatonin