title: Reduction of nitro phenols using nitroreductase from E. coli in the presence of NADH.
abstract: The reductions of nitrophenols catalyzed by nitroreductase from E. coli in the presence of NADH were investigated in this paper. 4-Aminophenol and 4-hydroxylaminophenol were found in the reductive products of 4-nitrophenol and the maximum reductive ratio was about 83.49% when the reaction time was 70 min; 4,6-dinitro-2-pimelie kelone was found in the reductive products of 2,4-dinitrophenol and the maximum reductive ratio was about 75.28% when the reaction time was 80 min; 2,4-dinitrophenol and 4,6-dinitro-2-pimelie kelone were found in the reductive products of 2,4,6-trinitrophenol and the maximum reductive ratio was about 62.08% when the reaction time was 100 min. The similar reductive ratios of nitrophenols were obtained under aerobic and anaerobic conditions. The results indicated that nitroreductase was an oxygen-insensitive enzyme.
authors.affiliation: School of Life Science and Technology, Beijing Institute of Technology, Beijing 100081, China. dairongji@bit.edu.cn
authors.forename: Rongji
authors.initials: R
authors.lastname: Dai
authors.forename: Jie
authors.initials: J
authors.lastname: Chen
authors.forename: Jie
authors.initials: J
authors.lastname: Lin
authors.forename: Shenyuan
authors.initials: S
authors.lastname: Xiao
authors.forename: Shi
authors.initials: S
authors.lastname: Chen
authors.forename: Yulin
authors.initials: Y
authors.lastname: Deng
mesh_terms: D019297:2,4-Dinitrophenol; D000627:Aminophenols; D001673:Biodegradation, Environmental; D004396:Coloring Agents; D029968:Escherichia coli Proteins; D015386:Hazardous Substances / Q000378:metabolism; D007700:Kinetics; D009153:Mutagens; D009243:NAD; D009596:Nitrophenols / Q000378:metabolism*; D009601:Nitroreductases / Q000378:metabolism*
chemical_list: D000627:Aminophenols; D004396:Coloring Agents; D029968:Escherichia coli Proteins; D015386:Hazardous Substances; D009153:Mutagens; D009596:Nitrophenols; D009243:NAD; D009601:Nitroreductases; D019297:2,4-Dinitrophenol; C026729:4-aminophenol