Grafting of silica with sulfobetaine polymers via aqueous reversible addition fragmentation chain transfer polymerization and its use as a stationary phase in HILIC.
Porous silica particles of 3 microm diameter and 100 A nominal pore size were first activated for vinylic polymerization by functionalization with 3-methacryloyloxypropyl trimethoxysilane (MAPTMS) and thereafter dressed with zwitterionic grafts of the sulfoalkylbetaine type in the "grafting through" fashion by polymerizing 3-(2-(N-methacryloyloxyethyl)-N,N-dimethylammonio)propane sulfonate (SPE), using either free radical polymerization or controlled reversible addition fragmentation chain transfer polymerization (RAFT). Particles polymerized using RAFT had a lower overall coating which seemed to be more evenly distributed in the pore volume. Both approaches resulted in columns with similar separation properties in HILIC mode.
Wikberg
Erika
E
Department of Chemistry, Umeå University, Umeå, Sweden.
Verhage
Jeroen J
JJ
Viklund
Camilla
C
Irgum
Knut
K
D000595:Amino Acid Sequence; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation* / Q000379:methods; D008422:Materials Testing; D008689:Methacrylates / Q000737:chemistry; D008969:Molecular Sequence Data; D015394:Molecular Structure; D009584:Nitrogen / Q000737:chemistry; D010316:Particle Size; D010455:Peptides / Q000032:analysis / Q000235:genetics; D011108:Polymers / Q000138:chemical synthesis / Q000737:chemistry*; D016062:Porosity; D012821:Silanes / Q000737:chemistry; D012822:Silicon Dioxide / Q000737:chemistry*; D013499:Surface Properties
C542237:3-methacryloxypropyltrimethoxysilane; D008689:Methacrylates; D010455:Peptides; D011108:Polymers; D012821:Silanes; D012822:Silicon Dioxide; D009584:Nitrogen