title: Improvement of Stearidonic acid production in Oleaginous Saccharomyces cerevisiae.
abstract: Rat Delta6 desaturase was introduced in "oleaginous" Saccharomyces cerevisiae genetically modified by the snf2 disruption, DGA1 overexpression, and LEU2 expression to improve stearidonic acid (18:4n-3 (Delta6, 9, 12, 15)) production. When this transformed yeast was cultured in the presence of 0.7 g/l alpha-linolenic acid for 7 d, 0.4 g of alpha-linolenic acid was accumulated and 44 mg of stearidonic acid was produced, mainly as a triacylglycerol in the 1 liter broth.
authors.affiliation: Institute for Biological Resources and Functions, National Institute of Advanced Industrial Science and Technology, Tsukuba, Ibaraki, Japan. kykimura@ni.aist.go.jp
authors.forename: Kazuyoshi
authors.initials: K
authors.lastname: Kimura
authors.forename: Nao
authors.initials: N
authors.lastname: Tomita
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Uemura
authors.forename: Tsunehiro
authors.initials: T
authors.lastname: Aki
authors.forename: Kazuhisa
authors.initials: K
authors.lastname: Ono
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Kamisaka
mesh_terms: D002849:Chromatography, Gas; D051048:Diacylglycerol O-Acyltransferase / Q000235:genetics; D015525:Fatty Acids, Omega-3 / Q000096:biosynthesis*; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics
chemical_list: D015525:Fatty Acids, Omega-3; D029701:Saccharomyces cerevisiae Proteins; C494880:DGA1 protein, S cerevisiae; D051048:Diacylglycerol O-Acyltransferase; C062895:stearidonic acid