Subcellular localization of rice histone deacetylases in organelles.
Histone deacetylases (HDACs) are known to function in the nucleus. Here, we report on the organellar localization of three rice HDACs, OsSIR2b, OsHDAC6, and OsHDAC10. The 35S:OsSIR2b-GFP and 35S:OsHDAC10-GFP constructs were introduced into tobacco BY2 cells. Co-localization analysis of the green fluorescent protein and MitoTracker fluorescent signals in the transformed BY2 cells indicated that OsSIR2b and OsHDAC10 are localized in the mitochondria. Transgenic Arabidopsis lines harboring 35S:OsHDAC6-GFP and 35S:OsHDAC10-GFP constructs were similarly analyzed, revealing that OsHDAC6-GFP is localized exclusively in chloroplasts, whereas OsHDAC10-GFP is localized in both mitochondria and chloroplasts. The presence of OsHDAC6-GFP and OsHDAC10-GFP in chloroplasts was verified by immunodetection.
Chung
Pil Joong
PJ
School of Biotechnology and Environmental Engineering, Myongji University, Yongin 449-728, Republic of Korea.
Kim
Youn Shic
YS
Park
Su-Hyun
SH
Nahm
Baek Hie
BH
Kim
Ju-Kon
JK
D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism; D002736:Chloroplasts / Q000201:enzymology*; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D006655:Histone Deacetylases / Q000032:analysis* / Q000378:metabolism; D008928:Mitochondria / Q000201:enzymology*; D015388:Organelles / Q000201:enzymology; D012275:Oryza / Q000201:enzymology*; D010940:Plant Proteins / Q000032:analysis* / Q000378:metabolism; D030821:Plants, Genetically Modified; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014026:Nicotiana / Q000235:genetics / Q000378:metabolism; D014162:Transfection
D010940:Plant Proteins; D049452:Green Fluorescent Proteins; D006655:Histone Deacetylases