[Mechanism of trichosanthin inducing apoptosis of mouse prostatic cancer RM-1 cells in vitro].
OBJECTIVE
To study the inducing effect of trichosanthin on the apoptosis of mouse prostatic cancer RM-1 cells and its mechanism.


METHODS
MTT assay was used to identify the effect of trichosanthin on RM-1 cells in vitro. The cells apoptosis was detected by Hoechest 33258 fluorescent staining and flow cytometry. The levels of bax proteins were detected by western blot analysis as well as their apoptosis rate.


RESULTS
The IC50 value of trichosanthin for RM-1 cell was 117.32 mg/L. Cell cycle was analysised by flow cytometry, trichosanthin induced an arrest in G0/G1 phase. Hoechest 33258 fluorescent staining showed typical nucleolus changes in apoptosis cells. Expression of bax was up-regulated at protein levels in a time-dependent manner.


CONCLUSIONS
Trichosanthin can induce apoptosis in RM-1 cells. The induction of apoptosis is a very important mechanism of trichosanthin to inhibit prostatic cancer.
Shi
Zhu
Z
Dept. of Urinary Surgery of Tongji Hospital of Tongji University, Shanghai 200065, China.
Shan
Sheng-dao
SD
Yuan
Tao
T
Gui
Ya-ping
YP
Cao
Cheng-hua
CH
Zhang
Jin-fu
JF
D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D015153:Blotting, Western; D002453:Cell Cycle / Q000187:drug effects; D045744:Cell Line, Tumor; D005434:Flow Cytometry; D015972:Gene Expression Regulation, Neoplastic; D020128:Inhibitory Concentration 50; D008297:Male; D051379:Mice; D018517:Plant Roots / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D011471:Prostatic Neoplasms / Q000378:metabolism / Q000473:pathology*; D015978:Trichosanthin / Q000494:pharmacology*; D051028:bcl-2-Associated X Protein / Q000378:metabolism
D000972:Antineoplastic Agents, Phytogenic; D051028:bcl-2-Associated X Protein; D015978:Trichosanthin