title: Klebsiella pneumoniae Carbapenemase-producing Enterobacteriaceae, Northeast Florida.
abstract: BACKGROUND
  Since 2001 there have been several reported outbreaks due to carbapenem-resistant Klebsiella pneumoniae (Kp), particularly in the northeastern states.
  
  
  METHODS
  Carbapenemase-producing Enterobacteriaceae from healthcare facilities in Northeast Florida were phenotypically identified and confirmed using PCR amplification and sequencing of the blaKPC gene.
  
  
  RESULTS
  Results from PFGE analysis of these isolates demonstrated possible horizontal spread from two possible "outbreak" strains during the study period.
  
  
  CONCLUSIONS
  We present the first published cluster of Kp and Escherichia coli (Ec) cases in Florida carrying the KPC-2 or KPC-3 gene.
authors.affiliation: Jacksonville Pathology Associates, PA and Baptist Health, Microbiology Laboratory, Memorial Hospital Jacksonville, Jacksonville, FL 32207, USA. s65@fdn.com
authors.forename: Diane C
authors.initials: DC
authors.lastname: Halstead
authors.forename: Timothy J
authors.initials: TJ
authors.lastname: Sellen
authors.forename: Jennifer M
authors.initials: JM
authors.lastname: Adams-Haduch
authors.forename: Deborah A
authors.initials: DA
authors.lastname: Dossenback
authors.forename: Joan
authors.initials: J
authors.lastname: Abid
authors.forename: Yohei
authors.initials: Y
authors.lastname: Doi
authors.forename: David L
authors.initials: DL
authors.lastname: Paterson
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D015780:Carbapenems / Q000378:metabolism; D004196:Disease Outbreaks; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000187:drug effects / Q000201:enzymology / Q000235:genetics; D004927:Escherichia coli Infections / Q000453:epidemiology / Q000382:microbiology; D005260:Female; D005431:Florida / Q000453:epidemiology; D005838:Genotype; D006801:Humans; D007710:Klebsiella Infections / Q000453:epidemiology* / Q000382:microbiology*; D007711:Klebsiella pneumoniae / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics; D008297:Male; D008826:Microbial Sensitivity Tests; D008875:Middle Aged; D055815:Young Adult; D001618:beta-Lactamases / Q000235:genetics / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D015780:Carbapenems; D001618:beta-Lactamases; C495220:beta-lactamase KPC-3, Klebsiella pneumoniae; C531581:carbapenemase-2, Klebsiella pneumoniae