title: [Parasitological, immunohistochemical and histopathological study for Leishmania chagasi detection in splenic tissues of dogs with visceral leishmaniasis].
abstract: The purpose of this work was a Canine Visceral Leishmaniasis--CVL study by parasitological direct examination of Leishmania (L.) chagasi (imprinting and histological), immunohistochemical test and histopathological analysis using spleen tissues from 34 dogs euthanized by the Zoonotic Disease Control Centre from Ilha Solteira, SP, Brazil. According to the clinical signs, the dogs were divided in three groups: asymptomatics (8 dogs), oligosymptomatics (17 dogs) and symptomatics (9 dogs). After the accomplishment of all diagnostic tests, 22 dogs were considered positives (64.7%) and 12 (35.3%) were negatives to CVL. From these positive dogs, 1/22 (4.5%) was asymptomatic, 12/22(54.5%) were oligosymptomatics and 8/22 (40.1%) were symptomatics. The histopathological study in spleen tissues from positive, especially symptomatic dogs, showed a diffuse chronic inflammation with thickness of capsular and trabecular regions and there was extensive morphologic alteration of the red and white pulp by the presence of abundant macrophages full with amastigotes, the granulomatous inflammatory reaction and haemorrhagic areas. The data of this work from histopathologic examination and direct microscopic visualization of L. (L.) chagasi showed that the spleen was an useful organ to collect sample tissues for CVL diagnosis. The immunostaining detected the highest number of positive dogs and were considered an important and conclusive method to be used in addition to parasitological methods for CVL, particularly in asymptomatic or oligosymptomatic dogs.
authors.affiliation: Departamento de Biologia e Zootecnia, Faculdade de Engenharia--FEIS, Universidade Estadual Paulista--UNESP, Brazil.
authors.forename: Karen Ingrid
authors.initials: KI
authors.lastname: Tasca
authors.forename: Wilma Aparecida Starke
authors.initials: WA
authors.lastname: Buzetti
authors.forename: Michely da Silva
authors.initials: Mda S
authors.lastname: Tenorio
authors.forename: Silvana de Cássia
authors.initials: Sde C
authors.lastname: Paulan
authors.forename: Flávia Luna
authors.initials: FL
authors.lastname: Lima
authors.forename: Nina Mari Gual Pimenta
authors.initials: NM
authors.lastname: de Queiroz
authors.forename: Rosângela Zacarias
authors.initials: RZ
authors.lastname: Machado
authors.forename: Tricia Maria Ferreira de Souza
authors.initials: TM
authors.lastname: Oliveira
authors.forename: Maria Francisca
authors.initials: MF
authors.lastname: Neves
authors.forename: Antonio Carlos Faconti
authors.initials: AC
authors.lastname: de Noronha
authors.forename: Juliana
authors.initials: J
authors.lastname: de Assis
mesh_terms: D000818:Animals; D004283:Dog Diseases / Q000469:parasitology* / Q000473:pathology*; D004285:Dogs; D007150:Immunohistochemistry; D018314:Leishmania infantum / Q000302:isolation & purification*; D007898:Leishmaniasis, Visceral / Q000469:parasitology* / Q000473:pathology* / Q000662:veterinary*; D013154:Spleen / Q000469:parasitology*