title: Multipotent CD15+ cancer stem cells in patched-1-deficient mouse medulloblastoma.
abstract: Subpopulations of tumorigenic cells have been identified in many human tumors, although these cells may not be very rare in some types of cancer. Here, we report that medulloblastomas arising from Patched-1-deficient mice contain a subpopulation of cells that show a neural precursor phenotype, clonogenic and multilineage differentiation capacity, activated Hedgehog signaling, wild-type Patched-1 expression, and the ability to initiate tumors following allogeneic orthotopic transplantation. The normal neural stem cell surface antigen CD15 enriches for the in vitro proliferative and in vivo tumorigenic potential from uncultured medulloblastomas, supporting the existence of a cancer stem cell hierarchy in this clinically relevant mouse model of cancer.
authors.affiliation: Developmental and Stem Cell Biology, Hospital for Sick Children, ON, Canada M5G 1X8.
authors.forename: Ryan J
authors.initials: RJ
authors.lastname: Ward
authors.forename: Lilian
authors.initials: L
authors.lastname: Lee
authors.forename: Kevin
authors.initials: K
authors.lastname: Graham
authors.forename: Thevagi
authors.initials: T
authors.lastname: Satkunendran
authors.forename: Koichi
authors.initials: K
authors.lastname: Yoshikawa
authors.forename: Erick
authors.initials: E
authors.lastname: Ling
authors.forename: Lauren
authors.initials: L
authors.lastname: Harper
authors.forename: Ryan
authors.initials: R
authors.lastname: Austin
authors.forename: Erica
authors.initials: E
authors.lastname: Nieuwenhuis
authors.forename: Ian D
authors.initials: ID
authors.lastname: Clarke
authors.forename: Chi-Chung
authors.initials: CC
authors.lastname: Hui
authors.forename: Peter B
authors.initials: PB
authors.lastname: Dirks
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D002528:Cerebellar Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D004622:Embryo, Mammalian; D005647:Fucosyltransferases / Q000378:metabolism; D016256:Lewis X Antigen / Q000378:metabolism*; D008527:Medulloblastoma / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D051379:Mice; D008810:Mice, Inbred C57BL; D016688:Mice, Inbred NOD; D016513:Mice, SCID; D008822:Mice, Transgenic; D008954:Models, Biological; D039902:Multipotent Stem Cells / Q000378:metabolism / Q000473:pathology*; D014411:Neoplastic Stem Cells / Q000378:metabolism / Q000473:pathology*; D000072079:Patched Receptors; D000072081:Patched-1 Receptor; D010641:Phenotype; D011956:Receptors, Cell Surface / Q000235:genetics* / Q000378:metabolism
chemical_list: D016256:Lewis X Antigen; C000606236:PTCH1 protein, human; D000072079:Patched Receptors; D000072081:Patched-1 Receptor; C000606240:Ptch1 protein, mouse; D011956:Receptors, Cell Surface; D005647:Fucosyltransferases; C541914:Fut4 protein, mouse