MicroRNA-1 regulates cardiomyocyte apoptosis by targeting Bcl-2.
MicroRNA-1 (miR-1) is preferentially expressed in cardiac muscles, and the expression has been demonstrated to be involved in cardiac development and cardiovascular diseases. Here we report that miR-1 is closely related with ischemia/reperfusion injury in a rat model. The level of miR-1 is inversely correlated with Bcl-2 protein expression in cardiomyocytes of the I/R rat model. In vitro, the level of miR-1 was dramatically increased in response to H(2)O(2). Overexpression of miR-1 facilitated H(2)O(2)-induced apoptosis in cardiomyocytes. Inhibition of miR-1 by antisense inhibitory oligonucleotides caused marked resistance to H(2)O(2). Through bioinformatics, we identified the potential target sites for miR-1 on the 3' UTR of Bcl-2. miR-1 significantly reduced the expression of Bcl-2 in the levels of mRNA and protein. The post-transcriptional repression of Bcl-2 was further confirmed by luciferase reporter experiments. These data demonstrated that miR-1 plays an important role in the regulation of cardiomyocyte apoptosis, which is involved in post-transcriptional repression of Bcl-2.
Tang
Yehua
Y
Department of Cardiology, Shanghai Changzheng Hospital, Second Military Medical University, Shanghai, China.
Zheng
Jiaoyang
J
Sun
Yan
Y
Wu
Zonggui
Z
Liu
Zhimin
Z
Huang
Gaozhong
G
D000818:Animals; D017209:Apoptosis / Q000235:genetics*; D002460:Cell Line; D005786:Gene Expression Regulation / Q000235:genetics; D035683:MicroRNAs / Q000235:genetics; D032383:Myocytes, Cardiac / Q000378:metabolism*; D011499:Protein Processing, Post-Translational / Q000235:genetics; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000235:genetics*; D051381:Rats; D020133:Reverse Transcriptase Polymerase Chain Reaction
C542191:MIRN1 microRNA, rat; D035683:MicroRNAs; D019253:Proto-Oncogene Proteins c-bcl-2