title: Genetic variant of CCND1: association with HPV-mediated cervical cancer in Indian population.
abstract: The potential association of single nucleotide polymorphisms (SNPs) (G870A and G1722C) of CCND1 with susceptibility to cervical cancer was investigated. The study included 200 cervical cancer cases along with an equal number of healthy controls. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis and direct sequencing were employed for genotyping. We found that women carrying the 870AA genotype have a 2.49-fold increased risk for the development of cervical cancer (odds ratio (OR) 2.49; 95% confidence interval (CI) 1.51-4.09; p = 0.0004) compared with GG+GA genotypes. For the 1722 locus, the frequency of the polymorphic 'C' allele was strongly associated with a reduced risk of cervical cancer (p = 0.019; OR 0.71; 95% CI 0.54-0.94). Our data suggest that CCND1 G870A polymorphism could act as a risk factor for the development of cervical cancer. And G1722C polymorphism may play a protective role against the development of human papillomavirus-associated cervical cancer among Indian women.
authors.affiliation: Divisions of Molecular Genetics & Biochemistry, Institute of Cytology & Preventive Oncology (ICMR), Noida, India.
authors.forename: Nisha
authors.initials: N
authors.lastname: Thakur
authors.forename: Showket
authors.initials: S
authors.lastname: Hussain
authors.forename: Indu
authors.initials: I
authors.lastname: Kohaar
authors.forename: Rubina
authors.initials: R
authors.lastname: Tabassum
authors.forename: Vilas
authors.initials: V
authors.lastname: Nasare
authors.forename: Pratibha
authors.initials: P
authors.lastname: Tiwari
authors.forename: Swaraj
authors.initials: S
authors.lastname: Batra
authors.forename: Suresh
authors.initials: S
authors.lastname: Bhambhani
authors.forename: Bhudev C
authors.initials: BC
authors.lastname: Das
authors.forename: Seemi Farhat
authors.initials: SF
authors.lastname: Basir
authors.forename: Dwaipayan
authors.initials: D
authors.lastname: Bharadwaj
authors.forename: Mausumi
authors.initials: M
authors.lastname: Bharadwaj
mesh_terms: D000328:Adult; D019938:Cyclin D1 / Q000235:genetics*; D005260:Female; D020022:Genetic Predisposition to Disease; D052162:Human papillomavirus 16 / Q000235:genetics*; D006801:Humans; D007194:India; D015810:Linkage Disequilibrium; D008875:Middle Aged; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide; D002583:Uterine Cervical Neoplasms / Q000235:genetics* / Q000821:virology*
chemical_list: C530104:CCND1 protein, human; D019938:Cyclin D1