title: Persistently elevated level of IL-8 in Chlamydia trachomatis infected HeLa 229 cells is dependent on intracellular available iron.
abstract: Chlamydia trachomatis is a leading cause of sexually transmitted infection worldwide and responsible for myriad of immunopathological changes associated with reproductive health. Delayed secretion of proinflammatory chemokine interleukin (IL)-8 is a hallmark of chlamydial infection and is dependent on chlamydial growth. We examined the effect of iron chelators on IL-8 production in HeLa 229 (cervix epitheloid cell, CCL2) cells infected with C. trachomatis. IL-8 production was induced by Iron chelator DFO and Mimosine, however, synergy with chlamydial infection was obtained with DFO only. Temporal expression of proinflammatory secreted cytokines IL-1beta, TNF-alpha, and IL-8 did not show synchrony in Chlamydia trachomatis infected cells. Secretion of IL-8 from Hela cells infected with C. trachomatis was not dependent on IL-1 beta and TNF- alpha induction. These results indicate towards involvement of iron in chlamydia induced IL-8 production.
authors.affiliation: Institute of Pathology (ICMR), Safdarjung Hospital Campus, New Delhi 110029, India.
authors.forename: Harsh
authors.initials: H
authors.lastname: Vardhan
authors.forename: Raini
authors.initials: R
authors.lastname: Dutta
authors.forename: Vikas
authors.initials: V
authors.lastname: Vats
authors.forename: Rishein
authors.initials: R
authors.lastname: Gupta
authors.forename: Rajneesh
authors.initials: R
authors.lastname: Jha
authors.forename: Hem Chandra
authors.initials: HC
authors.lastname: Jha
authors.forename: Pragya
authors.initials: P
authors.lastname: Srivastava
authors.forename: Apurb Rashmi
authors.initials: AR
authors.lastname: Bhengraj
authors.forename: Aruna
authors.initials: A
authors.lastname: Singh Mittal
mesh_terms: D002690:Chlamydia Infections / Q000378:metabolism*; D002692:Chlamydia trachomatis / Q000254:growth & development*; D004797:Enzyme-Linked Immunosorbent Assay; D006367:HeLa Cells; D006801:Humans; D053583:Interleukin-1beta / Q000378:metabolism; D016209:Interleukin-8 / Q000378:metabolism*; D007501:Iron / Q000378:metabolism*; D007502:Iron Chelating Agents / Q000494:pharmacology; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D053583:Interleukin-1beta; D016209:Interleukin-8; D007502:Iron Chelating Agents; D014409:Tumor Necrosis Factor-alpha; D007501:Iron