DNA preservation in skeletal elements from the World Trade Center disaster: recommendations for mass fatality management.
The World Trade Center (WTC) victim identification effort highlights taphonomic influences on the degradation of DNA from victims of mass fatality incidents. This study uses a subset of the WTC-Human Remains Database to evaluate differential preservation of DNA by skeletal element. Recovery location, sex, and victim type (civilian, firefighter, or plane passenger) do not appear to influence DNA preservation. Results indicate that more intact elements, as well as elements encased in soft tissue, produced slightly higher identification rates than more fragmented remains. DNA identification rates by element type conform to previous findings, with higher rates generally found in denser, weight-bearing bones. However, smaller bones including patellae, metatarsals, and foot phalanges yielded rates comparable to both femora and tibiae. These elements can be easily sampled with a disposable scalpel, and thus reduce potential DNA contamination. These findings have implications for DNA sampling guidelines in future mass fatality incidents.
Mundorff
Amy Z
AZ
Office of Chief Medical Examiner, New York City, NY, USA. amy_mundorff@sfu.ca
Bartelink
Eric J
EJ
Mar-Cash
Elaine
E
D001842:Bone and Bones / Q000737:chemistry; D004247:DNA / Q000302:isolation & purification*; D053836:DNA Degradation, Necrotic; D016172:DNA Fingerprinting / Q000379:methods*; D004209:Disposable Equipment; D005260:Female; D018732:Forensic Anthropology / Q000458:organization & administration; D006801:Humans; D008297:Male; D054527:Mass Casualty Incidents; D047851:September 11 Terrorist Attacks; D013048:Specimen Handling; D013525:Surgical Instruments
D004247:DNA