High-performance liquid chromatography with diode array detection coupled to electrospray time-of-flight and ion-trap tandem mass spectrometry to identify phenolic compounds from a lemon verbena extract.
High-performance liquid chromatography with diode array and electrospray ionization mass spectrometric detection was used to carry out the comprehensive characterization of a lemon verbena extract with demonstrated antioxidant and antiinflammatory activity. Two different MS techniques have been coupled to HPLC: on one hand, time-of-flight mass spectrometry, and on the other hand, tandem mass spectrometry on an ion-trap. The use of a small particle size C18 column (1.8 microm) provided a great resolution and made possible the separation of several isomers. The UV-visible spectrophotometry was used to delimit the class of phenolic compound and the accurate mass measurements on time-of-flight spectrometer enabled to identify the compounds present in the extract. Finally, the fragmentation pattern obtained in MS-MS experiments confirmed the proposed structures. This procedure was able to determine many well-known phenolic compounds present in lemon verbena such as verbascoside and its derivatives, diglucuronide derivatives of apigenin and luteolin, and eukovoside. Also gardoside, verbasoside, cistanoside F, theveside, campneoside I, chrysoeriol-7-diglucuronide, forsythoside A and acacetin-7-diglucuronide were found for the first time in lemon verbena.
Quirantes-Piné
R
R
Department of Analytical Chemistry, University of Granada, c/Fuentenueva s/n, 18003 Granada, Spain.
Funes
L
L
Micol
V
V
Segura-Carretero
A
A
Fernández-Gutiérrez
A
A
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005960:Glucosides / Q000302:isolation & purification; D010636:Phenols / Q000302:isolation & purification*; D010936:Plant Extracts / Q000302:isolation & purification*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D053719:Tandem Mass Spectrometry / Q000379:methods*; D032412:Verbena / Q000737:chemistry*; D059808:Polyphenols
D005960:Glucosides; D010636:Phenols; D010936:Plant Extracts; C058956:acteoside; D059808:Polyphenols