title: Studies on the production of nigerloxin using agro-industrial residues by solid-state fermentation.
abstract: Nigerloxin, a new and potent lipoxygenase inhibitor, was discovered in our laboratory through solid-state fermentation of wheat bran by Aspergillus niger V. Teigh (MTCC-5166). The aim of this study is to investigate the possibility of using different agro-industrial residues as nutritional supplements along with wheat bran to enhance the production of nigerloxin. Nigerloxin produced by SSF was quantified spectrophotometrically at 292 nm. The results indicate that the inhibitor production was influenced by the type of solid substrate supplemented, moisture content, pH and size of the inoculum. Individually optimized supplements were tested in different combinations to determine their effects on nigerloxin production. A twofold increase in the production of nigerloxin (4.9 +/- 0.3 mg gds(-1)) was achieved by supplementing wheat bran with 10% w/w sweet lemon peel and 5% v/w methanol at optimized process parameters, that is, an initial moisture content of 65% v/w and incubation period of 6 days with an initial inoculum size of 2 ml (8 x 10(5) spores gds(-1)). Nigerloxin production was stable between pH of 4 and 5.
authors.affiliation: Fermentation Technology and Bio Engineering Department, Central Food Technological Research Institute, Mysore 570020, India. dasagrandhichakradhar@gmail.com
authors.forename: Dasagrandhi
authors.initials: D
authors.lastname: Chakradhar
authors.forename: Saleem
authors.initials: S
authors.lastname: Javeed
authors.forename: Avinash P
authors.initials: AP
authors.lastname: Sattur
mesh_terms: D000383:Agriculture; D001234:Aspergillus niger / Q000254:growth & development / Q000302:isolation & purification / Q000378:metabolism*; D001565:Benzoates / Q000737:chemistry / Q000378:metabolism*; D018533:Biomass; D019149:Bioreactors; D001709:Biotechnology / Q000379:methods*; D002957:Citrus; D003470:Culture Media / Q000737:chemistry*; D004043:Dietary Fiber; D004791:Enzyme Inhibitors / Q000737:chemistry / Q000378:metabolism*; D005285:Fermentation; D006863:Hydrogen-Ion Concentration; D007220:Industrial Waste; D010105:Oxygenases / Q000037:antagonists & inhibitors; D011407:Propane / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism; D013379:Substrate Specificity; D014867:Water
chemical_list: D001565:Benzoates; D003470:Culture Media; D004043:Dietary Fiber; D004791:Enzyme Inhibitors; D007220:Industrial Waste; C469528:nigerloxin; D014867:Water; D010105:Oxygenases; D011407:Propane