Functional dissection of mitotic regulators through gene targeting in human somatic cells.
With the human genome fully sequenced (1, 2), biologists continue to face the challenging task of evaluating the function of each of the approximately 25,000 genes contained within it. Gene targeting in human cells provides a powerful and unique experimental tool in this regard (3-8). Although somewhat more involved than RNAi or pharmacological approaches, somatic cell gene targeting is a precise technique that avoids both incomplete knockdown and off-target effects, but is still much quicker than analogous manipulations in the mouse. Moreover, immortal knockout cell lines provide excellent platforms for both complementation analysis and biochemical purification of multiprotein complexes in native form. Here we present a detailed gene-targeting protocol that was recently applied to the mitotic regulator Polo-like kinase 1 (Plk1) (9).
Berdougo
Eli
E
Molecular Biology Program, Memorial Sloan-Kettering Cancer Center, New York, NY, USA.
Terret
Marie-Emilie
ME
Jallepalli
Prasad V
PV
D018797:Cell Cycle Proteins / Q000235:genetics* / Q000502:physiology; D002460:Cell Line; D018390:Gene Targeting / Q000379:methods*; D006801:Humans; D008938:Mitosis; D017346:Protein Serine-Threonine Kinases / Q000235:genetics* / Q000502:physiology; D011518:Proto-Oncogene Proteins / Q000235:genetics* / Q000502:physiology; D034741:RNA, Small Interfering; D011995:Recombination, Genetic; D000097869:Polo-Like Kinase 1
D018797:Cell Cycle Proteins; D017346:Protein Serine-Threonine Kinases; D011518:Proto-Oncogene Proteins; D034741:RNA, Small Interfering; D000097869:Polo-Like Kinase 1