title: Major proteins and antigens of Treponema denticola.
abstract: Treponema denticola is a Gram-negative, motile, asaccharolytic, anaerobic spirochaete which along with Porphyromonas gingivalis and Tannerella forsythia has been shown to form a bacterial consortium called the Red Complex that is strongly associated with the clinical progression of chronic periodontitis. T. denticola was grown in continuous culture in a complex medium with a mean generation time of 15.75 h. Samples from two different membrane-enriched preparations and a cytoplasm-enriched preparation were separated by two-dimensional gel electrophoresis and the proteins identified by MALDI-TOF/TOF mass spectrometry. In total, 219 non-redundant proteins were identified including numerous virulence factors, lipoproteins, ABC transporter proteins and enzymes involved in the metabolism of nine different amino acids of which glycine seems to be of particular importance. Novel findings include the identification of several abundant peptide uptake systems, and the identification of three flagellar filament outer layer proteins. Two-dimensional Western blot analysis using sera from mice immunized with formalin-killed T. denticola cells suggested that Msp, PrcA, OppA, OppA10, MglB, TmpC and several flagellar filament proteins are antigenic.
authors.affiliation: Melbourne Dental School, Bio21 Molecular Science and Biotechnology Institute, the University of Melbourne, Victoria, 3010, Australia.
authors.forename: Paul D
authors.initials: PD
authors.lastname: Veith
authors.forename: Stuart G
authors.initials: SG
authors.lastname: Dashper
authors.forename: Neil M
authors.initials: NM
authors.lastname: O'Brien-Simpson
authors.forename: Rita A
authors.initials: RA
authors.lastname: Paolini
authors.forename: Rebecca
authors.initials: R
authors.lastname: Orth
authors.forename: Katrina A
authors.initials: KA
authors.lastname: Walsh
authors.forename: Eric C
authors.initials: EC
authors.lastname: Reynolds
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis; D000942:Antigens, Bacterial / Q000302:isolation & purification*; D001426:Bacterial Proteins / Q000302:isolation & purification*; D015153:Blotting, Western; D002352:Carrier Proteins / Q000302:isolation & purification; D015180:Electrophoresis, Gel, Two-Dimensional; D004798:Enzymes / Q000302:isolation & purification; D005260:Female; D005407:Flagella / Q000737:chemistry; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D053719:Tandem Mass Spectrometry; D048373:Treponema denticola / Q000737:chemistry* / Q000254:growth & development / Q000276:immunology* / Q000472:pathogenicity
chemical_list: D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D002352:Carrier Proteins; D004798:Enzymes