Light-addressed stimulation under Ca(2+) imaging of cultured neurons.
Light addressing is an emerging and sophisticated technique that can induce pinpoint and/or patterned neuronal activation in cultured neurons. We previously developed a light-addressable electrode using hydrogenated amorphous silicon (a-Si:H), which was sandwiched between a tin oxide (SnO(2)) substrate and a passivation layer of zinc antimonate (ZnOSb(2)O(5)) dispersed epoxy. This research developed an experimental system that simultaneously implemented light-addressed stimulation and Ca(2+) imaging of neuronal activities. The translucent and thin laminated structure of our electrode permitted optical accesses from two directions: Ca(2+) imaging from above and light addressing from beneath. The submillisecond bright/dark switching property of our electrode offered light-addressed stimulation without causing interference with Ca(2+) imaging. To provide patterned illumination for light addressing, a digital micromirror device was installed in the system as an active photomask. The system could induce pinpoint neuronal activation at a cellular level.
Suzurikawa
Jun
J
Graduate School of Information Science and Technology, University of Tokyo, Tokyo, Japan. suzuri@brain.imi.i.u-tokyo.ac.jp
Nakao
Masayuki
M
Jimbo
Yasuhiko
Y
Kanzaki
Ryohei
R
Takahashi
Hirokazu
H
D000200:Action Potentials / Q000502:physiology; D000818:Animals; D002118:Calcium / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000166:cytology; D018920:Coculture Techniques; D004558:Electric Stimulation / Q000295:instrumentation* / Q000379:methods*; D004566:Electrodes; D004867:Equipment Design; D008027:Light; D008839:Microelectrodes; D009457:Neuroglia / Q000166:cytology / Q000502:physiology; D009474:Neurons / Q000166:cytology / Q000378:metabolism / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar; D012825:Silicon / Q000737:chemistry; D017971:Tin Compounds / Q000737:chemistry
D017971:Tin Compounds; C045358:stannic oxide; D002118:Calcium; D012825:Silicon