Leishmania infection inhibits cycloheximide-induced macrophage apoptosis in a strain-dependent manner.
Activation of apoptosis is one of the most ancient mechanisms to eliminate intracellular infections; the capacity to subvert this programed cell death provides an adaptive advantage to pathogens that persist in an intracellular environment. Leishmania species are obligate intracellular parasites that primarily reside within host macrophages. We demonstrate here that Leishmania infection protects macrophages from cycloheximide-induced apoptosis in a species and strain specific manner. Our data further reveal that Leishmania phosphoglycans and direct contact between parasites and host cells are required for the inhibitory phenotype.
Donovan
Michael J
MJ
Eck Institute for Global Health, Department of Biological Sciences, University of Notre Dame, Notre Dame, IN 46556, USA.
Maciuba
Britta Z
BZ
Mahan
Caitlin E
CE
McDowell
Mary Ann
MA
D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D002460:Cell Line; D003513:Cycloheximide / Q000494:pharmacology; D018320:Leishmania major / Q000502:physiology*; D008264:Macrophages / Q000166:cytology* / Q000469:parasitology*; D051379:Mice; D011500:Protein Synthesis Inhibitors / Q000494:pharmacology; D013045:Species Specificity
D011500:Protein Synthesis Inhibitors; D003513:Cycloheximide