Intracellular disruption of mitochondria in a living HeLa cell with a 76-MHz femtosecond laser oscillator.
Femtosecond laser pulses can be used to selectively disrupt and dissect intracellular organelles. We report on disruption of mitochondria in living HeLa cells using a femtosecond laser oscillator with a repetition rate of 76 MHz. We studied the laser parameters used for disruption. The longterm viability of the cells after disruption of a single mitochondrion was confirmed by the observation of cell division, indicating that intracellular disruption of organelles using a femtosecond laser oscillator can be performed without compromising the long-term cell viability.
Shimada
Tomoko
T
Watanabe
Wataru
W
Matsunaga
Sachihiro
S
Higashi
Tsunehito
T
Ishii
Hiroshi
H
Fukui
Kiichi
K
Isobe
Keisuke
K
Itoh
Kazuyoshi
K