Homo-D-lactic acid fermentation from arabinose by redirection of the phosphoketolase pathway to the pentose phosphate pathway in L-lactate dehydrogenase gene-deficient Lactobacillus plantarum.
Optically pure d-lactic acid fermentation from arabinose was achieved by using the Lactobacillus plantarum NCIMB 8826 strain whose l-lactate dehydrogenase gene was deficient and whose phosphoketolase gene was substituted with a heterologous transketolase gene. After 27 h of fermentation, 38.6 g/liter of d-lactic acid was produced from 50 g/liter of arabinose.
Okano
Kenji
K
Department of Chemical Science and Engineering, Kobe University, Rokkodaicho, Nada-ku, Japan.
Yoshida
Shogo
S
Tanaka
Tsutomu
T
Ogino
Chiaki
C
Fukuda
Hideki
H
Kondo
Akihiko
A
D000446:Aldehyde-Lyases / Q000378:metabolism; D001089:Arabinose / Q000378:metabolism*; D005285:Fermentation; D005818:Genetic Engineering / Q000379:methods*; D006801:Humans; D007770:L-Lactate Dehydrogenase / Q000172:deficiency; D019344:Lactic Acid / Q000378:metabolism*; D048191:Lactiplantibacillus plantarum / Q000235:genetics* / Q000378:metabolism*; D053858:Metabolic Networks and Pathways / Q000235:genetics*; D008954:Models, Biological; D010427:Pentose Phosphate Pathway / Q000235:genetics
D019344:Lactic Acid; D001089:Arabinose; D007770:L-Lactate Dehydrogenase; D000446:Aldehyde-Lyases; C022367:phosphoketolase