[Study on extraction of polyphenols from the leaves of Acer truncatum and their inhibitory abilities on fatty acid synthase].
OBJECTIVE
To optimize a technical process for extracting the polyphenols that can inhibit fatty acid synthase by ethanol from the leaves of Acer truncatum.


METHODS
The extracting time, extracting temperature, extracting solvent and the ratio of solvent to raw material were studied by L16 (4(5)) orthogonal experiments. The extraction rate of polyphenols and the inhibitory efficiency on the fatty acid synthase were utilized as evaluating criteria, and the optimum conditions for the extraction were obtained through summing up the above factors.


RESULTS
The efficient technological conditions were as follows: the concentration of ethanol was 60% (v/v), the ratio of solvent to raw material was 1 g: 30 mL; and the stirring time was 1.5 h at 50 degrees C.


CONCLUSIONS
The polyphenols that extracted from the leaves of Acer truncatum have inhibitory activity against fatty acid synthase and some of them have stronger inhibitory effect.
Ye
Yan-bin
YB
College of Chemical Biology and Pharmaceutical Sciences, Capital Medical University, Beijing 100069, China.
Yang
Shuai
S
Gao
Wen
W
Zhang
Feng
F
Guo
Jian-qiang
JQ
Zhang
Ying-xia
YX
D031002:Acer / Q000737:chemistry*; D000704:Analysis of Variance; D064429:Fatty Acid Synthases / Q000037:antagonists & inhibitors; D010636:Phenols / Q000032:analysis / Q000302:isolation & purification* / Q000494:pharmacology; D018515:Plant Leaves / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D013678:Technology, Pharmaceutical / Q000379:methods*; D013696:Temperature; D013997:Time Factors
D010636:Phenols; D064429:Fatty Acid Synthases