title: Sarcoglycan complex: implications for metabolic defects in muscular dystrophies.
abstract: The sarcoglycans are known as an integral subcomplex of the dystrophin glycoprotein complex, the function of which is best characterized in skeletal muscle in relation to muscular dystrophies. Here we demonstrate that the white adipocytes, which share a common precursor with the myocytes, express a cell-specific sarcoglycan complex containing beta-, delta-, and epsilon-sarcoglycan. In addition, the adipose sarcoglycan complex associates with sarcospan and laminin binding dystroglycan. Using multiple sarcoglycan null mouse models, we show that loss of alpha-sarcoglycan has no consequence on the expression of the adipocyte sarcoglycan complex. However, loss of beta- or delta-sarcoglycan leads to a concomitant loss of the sarcoglycan complex as well as sarcospan and a dramatic reduction in dystroglycan in adipocytes. We further demonstrate that beta-sarcoglycan null mice, which lack the sarcoglycan complex in adipose tissue and skeletal muscle, are glucose-intolerant and exhibit whole body insulin resistance specifically due to impaired insulin-stimulated glucose uptake in skeletal muscles. Thus, our data demonstrate a novel function of the sarcoglycan complex in whole body glucose homeostasis and skeletal muscle metabolism, suggesting that the impairment of the skeletal muscle metabolism influences the pathogenesis of muscular dystrophy.
authors.affiliation: Howard Hughes Medical Institute, University of Iowa Roy J. and Lucille A. Carver College of Medicine, Iowa City, Iowa 52242-1101, USA.
authors.forename: Séverine
authors.initials: S
authors.lastname: Groh
authors.forename: Haihong
authors.initials: H
authors.lastname: Zong
authors.forename: Matthew M
authors.initials: MM
authors.lastname: Goddeeris
authors.forename: Connie S
authors.initials: CS
authors.lastname: Lebakken
authors.forename: David
authors.initials: D
authors.lastname: Venzke
authors.forename: Jeffrey E
authors.initials: JE
authors.lastname: Pessin
authors.forename: Kevin P
authors.initials: KP
authors.lastname: Campbell
mesh_terms: D017667:Adipocytes / Q000378:metabolism; D000818:Animals; D015153:Blotting, Western; D049028:Dystrophin-Associated Protein Complex / Q000235:genetics / Q000378:metabolism*; D018149:Glucose Intolerance / Q000235:genetics / Q000378:metabolism; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D018482:Muscle, Skeletal / Q000378:metabolism; D009137:Muscular Dystrophy, Animal / Q000235:genetics / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D049031:Sarcoglycans / Q000235:genetics / Q000378:metabolism*
chemical_list: D049028:Dystrophin-Associated Protein Complex; D049031:Sarcoglycans