Two new flavonol glycosides from Epimedium koreanum Nakai.
Two new flavonol glycosides, named icarisid B (1) and icarisid C (2), along with seven known flavonol glycosides were isolated from the bioassay-directed fractions of the aqueous extract of Epimedium koreanum Nakai. The structures of the two new compounds were established on the basis of chemical and spectroscopic methods (ESI-MS, 1D, and 2D NMR) as 5-hydroxyl-4'-methoxy-8-(gamma-hydroxyl-gamma,gamma-dimethyl)-propyl-3-O-alpha-L-rhamnopyranosyl-flavonol-7-O-beta-D-glucopyranoside (1) and 5-hydroxyl-4'-methoxy-8-(gamma-methoxy-gamma,gamma-dimethyl)-propyl-3-O-beta-D-glucopyranosyl(1 --> 3)-alpha-L-rhamnopyranosyl-flavonol-7-O-beta-D-glucopyranoside (2), respectively. All the nine compounds were tested for their effects on proliferation and alkaline phosphatase (ALP) activity using UMR106 cells. As a result, five compounds showed stimulating effects on both the proliferation and ALP activity, which suggested that they might be used as potential leading compounds to cure osteoporosis.
Jiang
Fang
F
School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang, China.
Wang
Xin-Luan
XL
Wang
Nai-Li
NL
Yao
Xin-Sheng
XS
D000469:Alkaline Phosphatase / Q000187:drug effects*; D000818:Animals; D004195:Disease Models, Animal; D004354:Drug Screening Assays, Antitumor; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D034181:Epimedium / Q000737:chemistry*; D005419:Flavonoids / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D005960:Glucosides / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D051379:Mice; D015394:Molecular Structure; D010946:Plants, Medicinal / Q000737:chemistry*; D051381:Rats
D004365:Drugs, Chinese Herbal; D005419:Flavonoids; D005960:Glucosides; C541191:icarisid B; C541192:icarisid C; D000469:Alkaline Phosphatase