BSA-imprinted synthetic receptor for reversible template recognition.
A novel approach to the manufacturing of protein-responsive imprints on a home-made chitosan substrate was established together with m-aminophenylboronic acid (APBA) as a functional monomer. The produced polymers were characterized using both (1) equilibrium adsorption assays and (2) high performance liquid chromatography analysis. Results confirmed that the synthesized BSA-MIP (molecularly imprinted polymer) has a high affinity towards its template compared to the determined control proteins. The produced BSA-MIP featured largely in its good adsorption reversibility, especially in competitive binding assays, which is of great biological significance in separations. Non-specific binding was reduced to almost zero in a BSA/BHb competitive binding event. An excellent HPLC profile of template recognition was found for BSA-MIP, even under harsh mobile phase conditions. In the present work, the adopted trapped-template-release method permits recovery of bound BSA [1]. The strategy of making an artificial protein-receptor with high adsorption affinity and reversibility is promising in on-line isolation of target protein from complicated biological environments.
Wang
Huafang
H
Department of Chemistry, Nankai University, Tianjin, P.R. China.
He
Yunhua
Y
He
Xiwen
X
Li
Wenyou
W
Chen
Langxing
L
Zhang
Yukui
Y
D000327:Adsorption; D000818:Animals; D001667:Binding, Competitive; D001897:Boronic Acids / Q000737:chemistry*; D002417:Cattle; D048271:Chitosan / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D054802:Molecular Imprinting; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D013997:Time Factors
D001897:Boronic Acids; D012710:Serum Albumin, Bovine; C028592:3-aminobenzeneboronic acid; D048271:Chitosan