Detection of camel pox and vaccinia viruses by polymerase chain reaction.
PCR following two methods of DNA extraction was used to confirm the growth of camel pox virus (CPV) and vaccinia virus in cell culture and chorioallantoic membrane. Results were compared with the commonly used neutralization test. The first method of DNA extraction was accomplished by using viral DNA in tissue culture supernatant and Chorioallantoic membrane, which was released by initial heating for 15 min at 99 degrees C followed by ordinary PCR. In the second method DNA was extracted by using DNA Isolation Kit from tissue culture supernatant and used as a template. Rapid identification and differentiation of CPV and Vaccinia virus were achieved by PCR and this assay proved to be fast and feasible, and can be an alternative to orthodox serological methods.
Sheikh Ali
Hanan M
HM
Department of Microbiology, College of Veterinary Medicine & Animal Production, Sudan University of Science & Technology, P.O. Box: 204, Khartoum North, Sudan. diktora@yahoo.com
Khalafalla
A I
AI
Nimir
A H
AH
D000818:Animals; D002522:Chlorocebus aethiops; D003588:Cytopathogenic Effect, Viral; D009500:Neutralization Tests; D018155:Orthopoxvirus / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000379:methods*; D014616:Vaccinia virus / Q000302:isolation & purification*; D014709:Vero Cells