Immunoliposomal containers as systems of directed transport of minor interfering RNA into Schwann cells.
Immunoliposomal container system for targeted delivery of minor interfering RNA into Schwann cells was developed. Monoclonal antibodies to myelin basic protein served as the vector. Analysis by sandwich ELISA of myelin basic protein showed specific suppression of the target protein gene expression in Schwann cells after incubation with PEG-conjugated immunoliposomes loaded with minor interfering RNA blocking the synthesis of myelin basic protein. Prospects of effective use of this system for targeting of minor interfering RNA for the treatment of diseases of genetic etiology are discussed.
Chekhonin
V P
VP
V. P. Serbskii Center of Social and Forensic Psychiatry, Russian Ministry of Health, Moscow, Russia.
Tsibulkina
E A
EA
Ryabinina
A E
AE
Zhirkov
Yu A
YA
Savchenko
E A
EA
Shvets
V I
VI
D000818:Animals; D000911:Antibodies, Monoclonal / Q000737:chemistry / Q000235:genetics; D002478:Cells, Cultured; D004797:Enzyme-Linked Immunosorbent Assay; D005822:Genetic Vectors / Q000737:chemistry / Q000235:genetics; D006801:Humans; D008081:Liposomes / Q000737:chemistry*; D004676:Myelin Basic Protein / Q000235:genetics / Q000276:immunology; D011092:Polyethylene Glycols / Q000737:chemistry; D034741:RNA, Small Interfering / Q000737:chemistry / Q000235:genetics*; D051381:Rats; D012583:Schwann Cells / Q000378:metabolism*
D000911:Antibodies, Monoclonal; D008081:Liposomes; D004676:Myelin Basic Protein; D034741:RNA, Small Interfering; D011092:Polyethylene Glycols