title: Poly(methyl metacrylate) conductive fiber optic transducers as dual biosensor platforms.
abstract: Herein the development of an alternative optic-conductive fiber configuration applied for the construction of biosensing platforms. This new approach is based on applying the chemical polymerization of pyrrole onto the surface of polymethyl metacrylate (PMMA) fibers to create a polymer--a conductive surface, onto which an additional photoactive polypyrrole-benzophenone (PpyBz) film is electrochemically generated upon the fiber surface. Irradiation of the benzophenone groups embedded in the Ppy films with UV radiation (350 nm) formed active radicals that allowed the covalent attachment of the desired bioreceptors. Characterization of the amperometric biosensing matrix was accomplished by using a model Urease (Urs) through electrochemical impedance spectroscopy (EIS) and amperometry. Both techniques have shown a low charge transfer resistance (340 k Omega) and a high sensitivity (12.3 microA mM(-1)cm(-2)). Thereafter, the construction of an optical biosensing matrix based on horseradish peroxidase (HRP) production of photons was carried out. The high signal to noise (S/N) ratio (1600) indicated clearly that this approach can serve as a new platform to replace glass optical fibers based on biosensors.
authors.affiliation: Department of Virology, Ben-Gurion University of the Negev, P.O. Box 653, Beer-Sheva 84105, Israel.
authors.forename: Danit
authors.initials: D
authors.lastname: Atias
authors.forename: Khalil
authors.initials: K
authors.lastname: Abu-Rabeah
authors.forename: Sebastien
authors.initials: S
authors.lastname: Herrmann
authors.forename: Julia
authors.initials: J
authors.lastname: Frenkel
authors.forename: Dorith
authors.initials: D
authors.lastname: Tavor
authors.forename: Serge
authors.initials: S
authors.lastname: Cosnier
authors.forename: Robert S
authors.initials: RS
authors.lastname: Marks
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation*; D004553:Electric Conductivity; D004563:Electrochemistry / Q000295:instrumentation*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005336:Fiber Optic Technology / Q000295:instrumentation*; D008839:Microelectrodes; D019904:Polymethyl Methacrylate / Q000737:chemistry*; D014159:Transducers
chemical_list: D019904:Polymethyl Methacrylate