Simultaneous determination of 5-hydroxyindoles and catechols from urine using polymer monolith microextraction coupled to high-performance liquid chromatography with fluorescence detection.
To make analytes amenable for fluorescence (FL) detection, polymer monolith microextraction (PMME) coupled to high-performance liquid chromatography with FL detection was developed for the simultaneous determination of catechols and 5-hydroxyindoleamines (5-HIAs) from urine samples. In this method, a two-step pre-column derivatization method was employed to derivatize the analytes and a poly(methacrylic acid-co-ethylene glycol dimethacrylate) monolithic capillary column was used as the extraction medium for PMME. The conditions for the derivatization and subsequent extraction of 5-HIAs and catechols derivatives were optimized. Using our optimum conditions, the detection limit of the target analytes were 0.11-21 nM. Reproducibility of the method was obtained with intra-day and inter-day relative standard deviations less than 12% and a recovery of higher than 82%. In this study, we show how our proposed method can be used as a rapid sensitive technique for the determination of catechols and 5-HIAs from urine samples.
Wu
Yu-Bo
YB
Department of Chemistry, Wuhan University, Wuhan 430072, China.
Wu
Jian-Hong
JH
Shi
Zhi-Guo
ZG
Feng
Yu-Qi
YQ
D000328:Adult; D002396:Catechols / Q000652:urine*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005260:Female; D006801:Humans; D007211:Indoles / Q000652:urine*; D008297:Male; D008875:Middle Aged; D015203:Reproducibility of Results; D052617:Solid Phase Microextraction / Q000379:methods*; D013050:Spectrometry, Fluorescence / Q000379:methods*
D002396:Catechols; D007211:Indoles; C026785:5-hydroxyindole