title: Prenatal tobacco smoke exposure affects global and gene-specific DNA methylation.
abstract: BACKGROUND
  Prenatal exposure to tobacco smoke increases the risk for diseases later in the child's life that may be mediated through alterations in DNA methylation.
  
  
  OBJECTIVE
  To demonstrate that differences in DNA methylation patterns occur in children exposed to tobacco smoke and that variation in detoxification genes may alter these associations.
  
  
  METHODS
  Methylation of DNA repetitive elements, LINE1 and AluYb8, was measured using bisulfite conversion and pyrosequencing in buccal cells of 348 children participating in the Children's Health Study. Gene-specific CpG methylation differences associated with smoke exposure were screened in 272 participants in the Children's Health Study children using an Illumina GoldenGate panel. CpG loci that demonstrated a statistically significant difference in methylation were validated by pyrosequencing. Estimates were standardized across loci using a Z score to enable cross-comparison of results.
  
  
  RESULTS
  DNA methylation patterns were associated with in utero exposure to maternal smoking. Exposed children had significantly lower methylation of AluYb8 (beta, -0.31; P = 0.03). Differences in smoking-related effects on LINE1 methylation were observed in children with the common GSTM1 null genotype. Differential methylation of CpG loci in eight genes was identified through the screen. Two genes, AXL and PTPRO, were validated by pyrosequencing and showed significant increases in methylation of 0.37 (P = 0.005) and 0.34 (P = 0.02) in exposed children. The associations with maternal smoking varied by a common GSTP1 haplotype.
  
  
  CONCLUSIONS
  Life-long effects of in utero exposures may be mediated through alterations in DNA methylation. Variants in detoxification genes may modulate the effects of in utero exposure through epigenetic mechanisms.
authors.affiliation: Department of Preventive Medicine, University of Southern California, Keck School of Medicine, Los Angeles, CA 90033, USA.
authors.forename: Carrie V
authors.initials: CV
authors.lastname: Breton
authors.forename: Hyang-Min
authors.initials: HM
authors.lastname: Byun
authors.forename: Made
authors.initials: M
authors.lastname: Wenten
authors.forename: Fei
authors.initials: F
authors.lastname: Pan
authors.forename: Allen
authors.initials: A
authors.lastname: Yang
authors.forename: Frank D
authors.initials: FD
authors.lastname: Gilliland
mesh_terms: D002648:Child; D019175:DNA Methylation; D005260:Female; D005838:Genotype; D051549:Glutathione S-Transferase pi / Q000235:genetics*; D005982:Glutathione Transferase / Q000235:genetics*; D006801:Humans; D020084:Long Interspersed Nucleotide Elements / Q000187:drug effects / Q000235:genetics; D008297:Male; D018811:Maternal Exposure / Q000009:adverse effects*; D011247:Pregnancy; D011297:Prenatal Exposure Delayed Effects / Q000235:genetics*; D020086:Short Interspersed Nucleotide Elements / Q000187:drug effects / Q000235:genetics; D012907:Smoking / Q000009:adverse effects* / Q000235:genetics*; D014028:Tobacco Smoke Pollution / Q000009:adverse effects
chemical_list: D051549:Glutathione S-Transferase pi; D005982:Glutathione Transferase; D014028:Tobacco Smoke Pollution; C496556:GSTP1 protein, human