title: Amphiphilic perfluoroalkyl carbohydrates as new tools for liver imaging.
abstract: The synthesis of three molecules containing a fluorocarbon chain (either C(6)F(13), C(8)F(17) or C(10)F(21)), a sugar moiety (derived from lactobionic acid) and a chelate (derived from DTPA) is reported. These molecules (C(6)F(13)-Gal-DTPA, C(8)F(17)-Gal-DTPA or C(10)F(21)-Gal-DTPA) have been dispersed in water and their critical micellar concentration (CMC) as well as their size were determined. Their interaction with serum was weak as evaluated by time resolved fluorimetry of europium complexes. The presence of sugar on the surface of the nanoparticles was confirmed by the agglutination test using ricin. Conditions of pH and concentrations were optimised for in vivo studies. Finally, the nanoparticles formed with C(10)F(21)-Gal-DTPA have been complexed with (99m)Tc and injected to rats in order to follow their biodistribution by scintigraphy while following their stability by transmission electronic microscopy. A majority of the compound was found in the liver post-bolus injection.
authors.affiliation: Unité de Pharmacologie Chimique et Génétique, CNRS, UMR 8151, Paris, F-75270 cedex, France.
authors.forename: C
authors.initials: C
authors.lastname: Richard
authors.forename: P
authors.initials: P
authors.lastname: Chaumet-Riffaud
authors.forename: A
authors.initials: A
authors.lastname: Belland
authors.forename: A
authors.initials: A
authors.lastname: Parat
authors.forename: C
authors.initials: C
authors.lastname: Contino-Pepin
authors.forename: M
authors.initials: M
authors.lastname: Bessodes
authors.forename: D
authors.initials: D
authors.lastname: Scherman
authors.forename: B
authors.initials: B
authors.lastname: Pucci
authors.forename: N
authors.initials: N
authors.lastname: Mignet
mesh_terms: D000818:Animals; D002241:Carbohydrates / Q000737:chemistry*; D003952:Diagnostic Imaging / Q000379:methods* / Q000639:trends; D005466:Fluorocarbons / Q000737:chemistry*; D008099:Liver / Q000000981:diagnostic imaging* / Q000378:metabolism; D011877:Radionuclide Imaging; D051381:Rats; D013501:Surface-Active Agents / Q000737:chemistry*; D014018:Tissue Distribution / Q000502:physiology
chemical_list: D002241:Carbohydrates; D005466:Fluorocarbons; D013501:Surface-Active Agents