Assessing human immunodeficiency virus type 1 tropism: Comparison of assays using replication-competent virus versus plasma-derived pseudotyped virions.
Detection of CXCR4-using human immunodeficiency virus by the Trofile assay was compared to that by assays using virus isolates or replication-competent recombinants. Concordance with the Trofile assay was good, but assays using replicating viruses did not increase substantially the ability to detect the presence of CXCR4-using virus.
Hosoya
Noriaki
N
Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts, USA.
Su
Zhaohui
Z
Wilkin
Timothy
T
Gulick
Roy M
RM
Flexner
Charles
C
Hughes
Michael D
MD
Skolnik
Paul R
PR
Giguel
Françoise
F
Greaves
Wayne L
WL
Coakley
Eoin
E
Kuritzkes
Daniel R
DR
D015497:HIV-1 / Q000235:genetics / Q000502:physiology*; D006801:Humans; D010949:Plasma / Q000821:virology; D011991:Receptors, Virus / Q000032:analysis*; D012680:Sensitivity and Specificity; D014773:Virology / Q000379:methods*; D053586:Virus Internalization; D014779:Virus Replication
D011991:Receptors, Virus