UV Melting of G-Quadruplexes.
Absorbance versus temperature curves can provide information on the thermal stability of DNA or RNA quadruplexes. Quadruplex denaturation or renaturation can be followed by recording absorbance at 295 nm rather than 260 nm, the wavelength used to monitor duplex denaturation. This unit describes the use of absorbance versus temperature curves to determine melting temperatures (T(m) values) and model-dependent thermodynamic parameters.
Mergny
Jean-Louis
JL
Laboratoire de Biophysique, Inserm U565, Muséum National d'Histoire Naturelle, Paris, France.
Lacroix
Laurent
L
Laboratoire de Biophysique, Inserm U565, Muséum National d'Histoire Naturelle, Paris, France.
D054856:G-Quadruplexes / Q000528:radiation effects*; D009691:Nucleic Acid Denaturation / Q000528:radiation effects*; D013056:Spectrophotometry, Ultraviolet / Q000379:methods; D013696:Temperature; D014466:Ultraviolet Rays