title: Intracellular disruption of mitochondria in a living HeLa cell with a 76-MHz femtosecond laser oscillator.
abstract: Femtosecond laser pulses can be used to selectively disrupt and dissect intracellular organelles. We report on disruption of mitochondria in living HeLa cells using a femtosecond laser oscillator with a repetition rate of 76 MHz. We studied the laser parameters used for disruption. The longterm viability of the cells after disruption of a single mitochondrion was confirmed by the observation of cell division, indicating that intracellular disruption of organelles using a femtosecond laser oscillator can be performed without compromising the long-term cell viability.
authors.forename: Tomoko
authors.initials: T
authors.lastname: Shimada
authors.forename: Wataru
authors.initials: W
authors.lastname: Watanabe
authors.forename: Sachihiro
authors.initials: S
authors.lastname: Matsunaga
authors.forename: Tsunehito
authors.initials: T
authors.lastname: Higashi
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Ishii
authors.forename: Kiichi
authors.initials: K
authors.lastname: Fukui
authors.forename: Keisuke
authors.initials: K
authors.lastname: Isobe
authors.forename: Kazuyoshi
authors.initials: K
authors.lastname: Itoh