title: Identification of circulating tumour cells in early stage breast cancer patients using multi marker immunobead RT-PCR.
abstract: BACKGROUND
  The ability to screen blood of early stage operable breast cancer patients for circulating tumour cells is of potential importance for identifying patients at risk of developing distant relapse. We present the results of a study of the efficacy of the immunobead RT-PCR method in identifying patients with circulating tumour cells.
  
  
  RESULTS
  Immunomagnetic enrichment of circulating tumour cells followed by RT-PCR (immunobead RT-PCR) with a panel of five epithelial specific markers (ELF3, EPHB4, EGFR, MGB1 and TACSTD1) was used to screen for circulating tumour cells in the peripheral blood of 56 breast cancer patients. Twenty patients were positive for two or more RT-PCR markers, including seven patients who were node negative by conventional techniques. Significant increases in the frequency of marker positivity was seen in lymph node positive patients, in patients with high grade tumours and in patients with lymphovascular invasion. A strong trend towards improved disease free survival was seen for marker negative patients although it did not reach significance (p = 0.08).
  
  
  CONCLUSIONS
  Multi-marker immunobead RT-PCR analysis of peripheral blood is a robust assay that is capable of detecting circulating tumour cells in early stage breast cancer patients.
authors.affiliation: Department of Haematology/Oncology, The Queen Elizabeth Hospital, Adelaide, South Australia 5011, Australia. michael.raynor@adelaide.edu.au
authors.forename: Michael P
authors.initials: MP
authors.lastname: Raynor
authors.forename: Sally-Anne
authors.initials: SA
authors.lastname: Stephenson
authors.forename: Kenneth B
authors.initials: KB
authors.lastname: Pittman
authors.forename: David C A
authors.initials: DC
authors.lastname: Walsh
authors.forename: Michael A
authors.initials: MA
authors.lastname: Henderson
authors.forename: Alexander
authors.initials: A
authors.lastname: Dobrovic
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000032:analysis* / Q000235:genetics; D001943:Breast Neoplasms / Q000097:blood / Q000175:diagnosis* / Q000235:genetics / Q000473:pathology*; D045744:Cell Line, Tumor; D002469:Cell Separation / Q000379:methods; D055088:Early Detection of Cancer / Q000379:methods; D005260:Female; D006801:Humans; D007163:Immunosorbent Techniques; D008280:Magnetics / Q000379:methods; D008863:Microspheres; D008875:Middle Aged; D009367:Neoplasm Staging / Q000379:methods; D009360:Neoplastic Cells, Circulating / Q000737:chemistry / Q000473:pathology*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*
chemical_list: D014408:Biomarkers, Tumor