Synthesis, DNA-binding and photocleavage studies of [Ru(phen)2(pbtp)]2+ and [Ru(bpy)2(pbtp)]2+ (phen=1,10-phenanthroline; bpy=2,2'-bipyridine; pbtp=4,5,9,11,14-pentaaza-benzo[b]triphenylene).
Based on the ligand dppz (dppz=dipyrido-[3,2-a:2',3'-c]phenazine), a new ligand pbtp (pbtp=4,5,9,11,14-pentaaza-benzo[b]triphenylene) and its polypyridyl ruthenium(II) complexes [Ru(phen)(2)(pbtp)](2+) (1) (phen=1,10-phenanthroline and [Ru(bpy)(2)(pbtp)](2+) (2) (bpy=2,2'-bipyridine) have been synthesized and characterized by elemental analysis, ES-MS and (1)H NMR spectroscopy. The DNA-binding of these complexes were investigated by spectroscopic methods and viscosity measurements. The experimental results indicate that both complexes 1 and 2 bind to CT-DNA in classical intercalation mode, and can enantioselectively interact with CT-DNA. It is interesting to note that the pbtp ruthenium(II) complexes, in contrast to the analogous dppz complexes, do not show fluorescent behavior when intercalated into DNA. When irradiated at 365 nm, both complexes promote the photocleavage of pBR 322 DNA.
Tan
Lifeng
L
College of Chemistry, Xiangtan University, Xiangtan 411105, PR China. lfwyxh@yahoo.com.cn
Xiao
Yue
Y
Liu
Xiaohua
X
Zhang
Sheng
S
D000818:Animals; D001665:Binding Sites; D001667:Binding, Competitive; D002417:Cattle; D004247:DNA / Q000378:metabolism*; D007364:Intercalating Agents / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology*; D008024:Ligands; D015394:Molecular Structure; D009690:Nucleic Acid Conformation; D009942:Organometallic Compounds / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology*; D055668:Photochemical Processes; D013696:Temperature; D014783:Viscosity
C543397:(4,5,9,11,14-pentaazabenzo(b)triphenylene)bis(1,10-phenanthroline)ruthenium(II); D007364:Intercalating Agents; D008024:Ligands; D009942:Organometallic Compounds; C543398:bis(2,2'-bipyridine)(4,5,9,11,14-pentaazabenzo(b)triphenylene)ruthenium(II); D004247:DNA; C427203:calf thymus DNA