title: Leukoreduction by centrifugation does not eliminate Trypanosoma cruzi from infected blood units.
abstract: Current strategies to prevent transfusion-associated Chagas disease include the identification of Trypanosoma cruzi-infected blood donors through questionnaires and serologic tests. There are other procedures such as leukoreduction that prevent the transmission of infectious agents associated to white cells. The objective of the present work was to estimate the seroprevalence, evaluate the efficacy of leukoreduction by centrifugation to eliminate T. cruzi in infected blood units, and the correlation of immunoglobulin G (IgG) subclasses of seropositive blood donors with chronic chagasic cardiopathy. Over a period of 14 months, 33 out of 6600 blood donors (0.5%) at Centro Estatal de la Transfusión Sanguínea in Campeche State, México were seropositive for T. cruzi. Twenty seropositive blood units were submitted through leukoreduction by centrifugation, and in the fractions generated (red cell fraction, platelets, and the buffy-coat), we searched for the presence of T. cruzi using specific polymerase chain reaction. We detected parasite DNA in 50% to 60% of the fractions tested, suggesting that leukoreduction by centrifugation does not eliminate the microorganisms in the infected blood unit. We also observed that the level of IgG2 and IgG4 subclasses specific for T. cruzi in seropositive blood donors was lower than in chronic cardiopathic chagasic patients. In conclusion, leukoreduction by centrifugation has a limited role in eliminating T. cruzi in infected blood supply, and the low level of specific IgG2 and IgG4 could be a marker in the indeterminate phase of infection.
authors.affiliation: Centro Estatal de la Transfusión Sanguínea de Campeche, SSA, Campeche, Mexico.
authors.forename: Doris
authors.initials: D
authors.lastname: Dzib
authors.forename: Virginia Peña
authors.initials: VP
authors.lastname: Hernández
authors.forename: Baldemar Canche
authors.initials: BC
authors.lastname: Ake
authors.forename: Ruth Alacantara
authors.initials: RA
authors.lastname: López
authors.forename: Victor Manuel
authors.initials: VM
authors.lastname: Monteón
mesh_terms: D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood; D001771:Blood Banks; D001782:Blood Donors; D002498:Centrifugation / Q000379:methods*; D014355:Chagas Disease / Q000453:epidemiology* / Q000635:transmission; D006801:Humans; D008800:Mexico / Q000453:epidemiology; D016036:Seroepidemiologic Studies; D065227:Transfusion Reaction; D014349:Trypanosoma cruzi
chemical_list: D000913:Antibodies, Protozoan