title: Effect of dextran 500 on radial migration of erythrocytes in postcapillary venules at low flow rates.
abstract: Recently, we reported that collision efficiency (fraction of total collisions that result in the formation of aggregates) between red blood cells was an important factor in the formation of aggregates in postcapillary venules. In the present study, we focus on how high molecular weight dextran influences the overall radial migration trend of red blood cells in the postcapillary venule along a longitudinal distance of 50 microm from the bifurcation which would in turn affect collision behavior of these cells. A radial migration index, which defines the extent of radial migration of individual cells relative to the vessel center, was found to have a larger magnitude after infusion of dextran (1.9 +/- 2.73) compared to that before dextran infusion (1.48 +/- 3.89). This implied that dextran-induced aggregation might provide an external force to actively move cells towards the centerline of the vessel, which could contribute to the greater number of red blood cells participating in collision (16% increase) and aggregate formation. Further analysis of the collision behavior of individual red blood cells revealed that collision frequencies of individual cells decreased from a wide range (1 to 14) to a narrow range (1 to 5) after dextran treatment, indicating the alteration of collision behavior of red blood cells by the presence of aggregates along the flow stream.
authors.affiliation: Division of Bioengineering, National University of Singapore, Singapore.
authors.forename: Sangho
authors.initials: S
authors.lastname: Kim
authors.forename: Peng Kai
authors.initials: PK
authors.lastname: Ong
authors.forename: Paul C
authors.initials: PC
authors.lastname: Johnson
mesh_terms: D000818:Animals; D000925:Anticoagulants / Q000008:administration & dosage / Q000494:pharmacology; D001783:Blood Flow Velocity / Q000187:drug effects; D001794:Blood Pressure / Q000187:drug effects; D002196:Capillaries / Q000187:drug effects / Q000502:physiology; D002465:Cell Movement / Q000187:drug effects*; D003911:Dextrans / Q000008:administration & dosage / Q000494:pharmacology*; D004903:Erythrocyte Aggregation / Q000187:drug effects*; D004912:Erythrocytes / Q000166:cytology / Q000187:drug effects* / Q000502:physiology; D006439:Hemodynamics / Q000187:drug effects; D008297:Male; D008954:Models, Biological; D051381:Rats; D013997:Time Factors; D014699:Venules / Q000187:drug effects / Q000502:physiology
chemical_list: D000925:Anticoagulants; D003911:Dextrans