Structural characterization of dietary fiber of green chalcumra (Benincasa hispida) fruit by NMR spectroscopic analysis.
A water-soluble dietary fiber was isolated from the hot aqueous extract of Chalcumra (Benincasa hispida) fruit. The polysaccharide was found to contain D-galactose and D-methyl galacturonate in a molar ratio of 2:1. On the basis of acid hydrolysis, methylation analysis, periodate oxidation, and NMR spectroscopic studies (1H, 13C, TOCSY, DQF-COSY, NOESY, ROESY, HMQC, and HMBC), the repeating unit of the polysaccharide was established as -->4)-beta-D-Galp-(1-->4)-beta-D-Galp-(1-->2)-alpha-D-GalpA6Me-( 1-->.
Das
Debsankar
D
Department of Chemistry and Chemical Technology, Vidyasagar University, Midnapore 721102, West Bengal, India.
Mondal
Subhas
S
Maiti
Debabrata
D
Roy
Sadhan K
SK
Islam
Syed S
SS
D002236:Carbohydrate Conformation; D019662:Cucurbitaceae / Q000737:chemistry*; D004043:Dietary Fiber; D005638:Fruit / Q000737:chemistry*; D005690:Galactose / Q000737:chemistry / Q000302:isolation & purification; D006603:Hexuronic Acids / Q000737:chemistry / Q000302:isolation & purification; D019906:Nuclear Magnetic Resonance, Biomolecular; D011134:Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification
D004043:Dietary Fiber; D006603:Hexuronic Acids; D011134:Polysaccharides; C007819:galacturonic acid; D005690:Galactose