title: Degradation of rutin by Thermoactinomyces vulgaris and other thermophilic compost isolates.
abstract: The mutagenic effects of rutin and quercetin have aroused the interest of many investigators. To develop the microbial degradation of rutin, a thermophilic actinomycete, which could hydrolyze rutin, was isolated from compost soil. The taxonomical characteristics of this thermophilic actinomycete were examined and identified as Thermoactinomyces vulgaris PU18-2. After cultivation of T. vulgaris PU18-2 in the rutin-CYC medium for 60 h, the culture filtrate had a rutin-degrading ability, but the cell-free extract did not. There was no quercetin, rutinose, rhamnose, and glucose accumulated in the rutin hydrolysate of the culture filtrate. Both alpha-rhamnosidase and beta-glucosidase activities were not found in the culture filtrate of the T. vulgaris PU18-2 in the rutin-CYC medium. These results showed that the initial attack on rutin by the extracellular enzymes of T. vulgaris PU18-2 apparently was not through the glycosidase-mediated hydrolysis of glycosidic bond.
authors.affiliation: Department of Cosmetic Science, Providence University, Shalu, Taichung, Taiwan. chyang@pu.edu.tw
authors.forename: Chao-Hsun
authors.initials: CH
authors.lastname: Yang
authors.forename: Yu-Chun
authors.initials: YC
authors.lastname: Huang
authors.forename: Cheng-Yu
authors.initials: CY
authors.lastname: Chen
mesh_terms: D001426:Bacterial Proteins / Q000378:metabolism; D006026:Glycoside Hydrolases / Q000378:metabolism; D006868:Hydrolysis; D008848:Micromonosporaceae / Q000201:enzymology / Q000302:isolation & purification / Q000378:metabolism*; D012431:Rutin / Q000378:metabolism*; D012987:Soil / Q000032:analysis*; D012988:Soil Microbiology; D001617:beta-Glucosidase / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D012987:Soil; D012431:Rutin; D006026:Glycoside Hydrolases; D001617:beta-Glucosidase; C022525:alpha-L-rhamnosidase