Analysis of insoluble proteins.
The analysis of insoluble proteins represents a major technical challenge for the field of proteomics. For example, membrane proteins are often insoluble in common solvents and represent 20-30% of the proteins encoded by the human genome. Chemical analysis on an individual basis is often required and is laborious and time-consuming. This review presents an overview of methods for purification of expressed proteins using fusion tags as well as methods for analysis of insoluble proteins by mass spectrometry with a goal of achieving high-throughput analysis.
Trimpin
Sarah
S
Department of Chemistry, Wayne State University, Department of Chemistry, Detroit, Michigan, USA.
Brizzard
Bill
B
D000818:Animals; D006801:Humans; D013058:Mass Spectrometry; D011506:Proteins / Q000032:analysis* / Q000737:chemistry*; D040901:Proteomics; D011994:Recombinant Proteins / Q000302:isolation & purification / Q000378:metabolism; D012995:Solubility; D012997:Solvents
D011506:Proteins; D011994:Recombinant Proteins; D012997:Solvents