Preparative purification of morroniside and loganin from Fructus corni by combination of macroporous absorption resin and HSCCC.
A method for preparative purification of loganin and morroniside from Fructus corni was established by combination of macroporous absorption resin column separation and high-speed counter-current chromatography (HSCCC). The crude extracts obtained from Fructus corni by ultrasonic extraction with 50% methanol were subjected to separation on a macroporous resin column and then eluted with 15% and 40% ethanol, respectively. A fraction of 40% ethanol was used as the sample for separation of morroniside and loganin by HSCCC. The two-phase solvent system used for HSCCC separation was n-butanol-methanol-1% acetic acid water (4:1:6, v/v). The upper phase was used as the stationary phase of HSCCC. Morroniside (28.7 mg) of 97.8% purity and loganin (11.5 mg) of 98.6% purity were obtained in a one-step HSCCC separation from 50 mg of sample. The structures of morroniside and loganin were identified by 1H-NMR and 13C-NMR.
Liu
Liangpo
L
College of Chemistry and Chemical Engineering, Liaocheng University, Liaocheng, 252059, PR China.
Sun
Ailing
A
Wu
Sujuan
S
Liu
Renmin
R
D000042:Absorption; D002851:Chromatography, High Pressure Liquid; D003377:Countercurrent Distribution / Q000379:methods*; D006027:Glycosides / Q000302:isolation & purification*; D039823:Iridoids / Q000302:isolation & purification*; D009682:Magnetic Resonance Spectroscopy; D010936:Plant Extracts / Q000737:chemistry*
D006027:Glycosides; D039823:Iridoids; D010936:Plant Extracts; C488401:morroniside; C059516:loganin