title: Dy3+ and Nd3+ induced genetic mutation of bacillus alpha-amylase.
abstract: After treatment with DyCl(3) and NdCl(3), two strains of mutated bacilli showing increased alpha-amylase activity were isolated. The alpha-amylase genes were amplified, cloned, and sequenced. Sequencing revealed that there were either 11 or 14 bases altered in the two genes. These alterations took the form of base substitutions, and transversion was more common than transition. Based on these results, it was concluded that the rare earth compounds DyCl(3) and NdCl(3) were mutagens.
authors.affiliation: School of Chemical Engineering, Department of Biological Engineering, Inner Mongolia University of Technology, Hohhot, China. xym@imut.edu.cn
authors.forename: Dongyan
authors.initials: D
authors.lastname: Zhang
authors.forename: Jianhua
authors.initials: J
authors.lastname: Hu
authors.forename: Bater
authors.initials: B
authors.lastname: Siqin
authors.forename: Tong
authors.initials: T
authors.lastname: Zhang
authors.forename: Ying
authors.initials: Y
authors.lastname: Chang
mesh_terms: D001412:Bacillus subtilis / Q000187:drug effects* / Q000201:enzymology / Q000235:genetics; D001483:Base Sequence; D003001:Cloning, Molecular; D004419:Dysprosium / Q000494:pharmacology*; D008969:Molecular Sequence Data; D009153:Mutagens / Q000494:pharmacology*; D009154:Mutation; D009354:Neodymium / Q000494:pharmacology*; D000516:alpha-Amylases / Q000096:biosynthesis / Q000235:genetics*
chemical_list: D009153:Mutagens; D004419:Dysprosium; C053739:neodymium chloride; D009354:Neodymium; D000516:alpha-Amylases