title: Pattern recognition analysis for 1H NMR spectra of plasma from hemodialysis patients.
abstract: 1H NMR spectroscopic and pattern recognition-based methods (NMR-PR) were applied to the metabolic profiling studies on hemodialysis (HD). Plasma samples were collected from 37 patients before and after HD and measured by 600 MHz NMR spectroscopy. Each spectrum was data-processed and subjected to principal component analysis for pattern recognition. Spectral patterns of plasma between pre- and post-dialyses were clearly discriminated, together with significant fluctuations in the levels of creatinine, trimethylamine-N-oxide, glucose, lactate, and acetate, which were quantitated. We have first observed the significant elevation of lactate levels in post-dialysis plasma. The present study has demonstrated the high feasibility of NMR-PR method for monitoring the dialysis condition and comprehensive profiling of the change of low-molecular-weight metabolites in HD.
authors.affiliation: Graduate School of Pharmaceutical Sciences, Tohoku University, 6-3, Aramaki-aza-aoba, Aoba-ku, Sendai 980-8578, Japan. fmasako@m.tains.tohoku.ac.jp
authors.forename: Masako
authors.initials: M
authors.lastname: Fujiwara
authors.forename: Takeshi
authors.initials: T
authors.lastname: Kobayashi
authors.forename: Takahiro
authors.initials: T
authors.lastname: Jomori
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Maruyama
authors.forename: Yoshitomo
authors.initials: Y
authors.lastname: Oka
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Sekino
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Imai
authors.forename: Kazuhisa
authors.initials: K
authors.lastname: Takeuchi
mesh_terms: D000085:Acetates / Q000097:blood; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001786:Blood Glucose / Q000378:metabolism; D003401:Creatine / Q000097:blood; D005260:Female; D006801:Humans; D019344:Lactic Acid / Q000097:blood; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D008297:Male; D055442:Metabolome; D008744:Methylamines / Q000097:blood; D008875:Middle Aged; D011522:Protons; D006435:Renal Dialysis
chemical_list: D000085:Acetates; D001786:Blood Glucose; D008744:Methylamines; D011522:Protons; D019344:Lactic Acid; C005855:trimethyloxamine; D003401:Creatine