title: [Experimental study on prevention of postsurgical adhesions by using O-carboxymethylchitosan solution after an abdominal operation].
abstract: O-Carboxymethylchitosan (O-CMC) in 1000 g batch was prepared from chitin as starting material and its chemical structure was confirmed by analysis of IR and NMR. O-CMC solution, sodium hyaluronate (HA) solution and physiological saline were used in Sprague-Dawley rat model for prevention of postsurgical adhesions; after 7 days of an abdominal operation, the 3 groups were evaluated according to Belluco standard, the mean scores of O-CMC group, HA group and physiological saline group were 2.5 +/- 3.1, 3.3 +/- 3.6 and 10.3 +/- 1.0, respectively. Histological inspection showed that in O-CMC group, mesothelial cells on peritonaeum or cecum surfaces were almost restored; in HA group the injured surface of peritonaeum was mostly repaired, but in physiological saline group the injured surface of cecum was just a little repaired; there were extensive adhesions between peritonaeum and cecum, and inflammatory response was quite serious. Experimental results indicated that O-CMC and HA had excellent efficiency and O-CMC was slightly better than HA for the prevention of postsurgical adhesions.
authors.affiliation: The First Affiliated Hospital, Sun Yat-Sen University, Guangzhou 510080, China. gzpshr@163.com
authors.forename: Shirong
authors.initials: S
authors.lastname: Pan
authors.forename: Jiacong
authors.initials: J
authors.lastname: Mo
authors.forename: Huanling
authors.initials: H
authors.lastname: Zheng
authors.forename: Wu
authors.initials: W
authors.lastname: Yi
authors.forename: Daquan
authors.initials: D
authors.lastname: Huang
authors.forename: Bi
authors.initials: B
authors.lastname: Wang
mesh_terms: D000005:Abdomen / Q000601:surgery*; D000818:Animals; D048271:Chitosan / Q000031:analogs & derivatives* / Q000627:therapeutic use; D005260:Female; D007410:Intestinal Diseases / Q000517:prevention & control*; D008297:Male; D011183:Postoperative Complications / Q000517:prevention & control*; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D000267:Tissue Adhesions / Q000517:prevention & control
chemical_list: C504164:O-carboxymethylchitosan; D048271:Chitosan