Production of plant-specific tyramine derivatives by dual expression of tyramine N-hydroxycinnamoyltransferase and 4-coumarate:coenzyme A ligase in Escherichia coli.
Plant-specific bioactive compounds including feruloyltyramine (FT), 4-coumaroyltyramine (CT), and caffeoyltyramine (CaT) were simultaneously produced in Escherichia coli by heterologous expression of two biosynthetic genes encoding 4-coumarate:coenzyme A ligase and tyramine N-hydroxycinnamoyltransferase (THT) cloned from Arabidopsis thaliana and pepper, respectively. Simultaneous supplementation of substrates to the recombinant E. coli resulted in the secretion of multiple tyramine derivatives into the medium at high yield: CT (189 mg l(-1)), FT (135 mg l(-1)), CaT (40 mg l(-1)). In addition, the recombinant E. coli also produced, albeit at low concentration, a range of dopamine derivatives such as feruloyldopamine due to THT's ability to accept dopamine as a substrate.
Kang
Kiyoon
K
Department of Biotechnology, Interdisciplinary Program for Bioenergy and Biomaterials of Graduate School, Biotechnology Research Institute, Chonnam National University, Gwangju 500-757, Republic of Korea.
Park
Munyoung
M
Park
Sangkyu
S
Kim
Young Soon
YS
Lee
Sungbeom
S
Lee
Seong-Gene
SG
Back
Kyoungwhan
K
D000217:Acyltransferases / Q000235:genetics* / Q000378:metabolism*; D017360:Arabidopsis / Q000201:enzymology* / Q000235:genetics; D002109:Caffeic Acids / Q000378:metabolism; D003066:Coenzyme A Ligases / Q000235:genetics* / Q000378:metabolism*; D003373:Coumaric Acids / Q000378:metabolism; D003470:Culture Media / Q000737:chemistry; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D015870:Gene Expression; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D014439:Tyramine / Q000031:analogs & derivatives* / Q000378:metabolism*
D002109:Caffeic Acids; D003373:Coumaric Acids; D003470:Culture Media; C001411:N-(4-hydroxy-beta-phenethyl)-4-hydroxycinnamide; D011994:Recombinant Proteins; C481438:N-caffeoyltyramine; C074004:feruloyltyramine; D000217:Acyltransferases; C108168:hydroxycinnamoyl-CoA-tyramine N-(hydroxycinnamoyl)transferase; D003066:Coenzyme A Ligases; C019943:4-coumarate-CoA ligase; D014439:Tyramine