Cloning, expression, purification, crystallization and preliminary X-ray crystallographic analysis of interleukin-4-inducing principle from Schistosoma mansoni eggs (IPSE/alpha-1).
The interleukin-4-inducing principle from Schistosoma mansoni eggs (IPSE/alpha-1) triggers the release of large amounts of interleukin-4 from human blood basophils, thus presumably playing an immunomodulatory role during schistosome infection. IPSE/alpha-1 was crystallized and a native X-ray data set was collected to 1.66 A resolution from a single crystal at 100 K using synchrotron radiation. The crystal belonged to space group P6(1) or P6(5), with one molecule per asymmetric unit.
Mayerhofer
Hubert
H
EMBL Hamburg Outstation, c/o DESY, Notkestrasse 85, D-22603 Hamburg, Germany.
Schramm
Gabriele
G
Hatzopoulos
Georgios N
GN
Mueller-Dieckmann
Christoph
C
Haas
Helmut
H
Mueller-Dieckmann
Jochen
J
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D003460:Crystallization; D003545:Cysteine / Q000737:chemistry; D003625:Data Collection; D019281:Dimerization; D004220:Disulfides / Q000737:chemistry; D004527:Egg Proteins / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics; D005260:Female; D015801:Helminth Proteins / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*; D006639:Histidine / Q000737:chemistry; D015847:Interleukin-4 / Q000378:metabolism*; D008969:Molecular Sequence Data; D009154:Mutation; D019913:Nuclear Localization Signals; D011993:Recombinant Fusion Proteins / Q000302:isolation & purification / Q000378:metabolism; D012550:Schistosoma mansoni / Q000235:genetics / Q000302:isolation & purification*; D013223:Statistics as Topic; D013696:Temperature; D014961:X-Ray Diffraction
D004220:Disulfides; D004527:Egg Proteins; D015801:Helminth Proteins; C474807:IPSE protein, Schistosoma mansoni; D019913:Nuclear Localization Signals; D011993:Recombinant Fusion Proteins; D015847:Interleukin-4; D006639:Histidine; D003545:Cysteine