title: Cloning, overproduction, purification, crystallization and preliminary X-ray diffraction analysis of yeast glutaredoxin Grx5.
abstract: Grx5 from the yeast Saccharomyces cerevisiae is a monothiol glutaredoxin that is involved in iron-sulfur cluster biogenesis. Here, yeast Grx5 was cloned and overproduced in Escherichia coli. The purified protein was crystallized using the hanging-drop vapour-diffusion method. Diffraction data for Grx5 were collected to 1.67 A resolution. The crystal of Grx5 belonged to space group R3, with unit-cell parameters a = b = 85.12, c = 48.95 A, alpha = beta = 90.00, gamma = 120.00 degrees .
authors.affiliation: Hefei National Laboratory for Physical Sciences at Microscale and School of Life Sciences, University of Science and Technology of China, Hefei, Anhui 230026, People's Republic of China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Wang
authors.forename: Yong Xing
authors.initials: YX
authors.lastname: He
authors.forename: Jiang
authors.initials: J
authors.lastname: Yu
authors.forename: Cong Zhao
authors.initials: CZ
authors.lastname: Zhou
mesh_terms: D003001:Cloning, Molecular; D003460:Crystallization; D004271:DNA, Fungal / Q000235:genetics; D003625:Data Collection; D004926:Escherichia coli / Q000235:genetics; D005822:Genetic Vectors; D054477:Glutaredoxins / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D010957:Plasmids; D012441:Saccharomyces cerevisiae / Q000737:chemistry*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*; D013223:Statistics as Topic; D013696:Temperature; D013698:Templates, Genetic; D013997:Time Factors; D014169:Transformation, Bacterial; D014961:X-Ray Diffraction
chemical_list: D004271:DNA, Fungal; D054477:Glutaredoxins; C528773:Grx5 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins