title: Clostridium tagluense sp. nov., a psychrotolerant, anaerobic, spore-forming bacterium from permafrost.
abstract: A strictly anaerobic, Gram-positive, psychrotolerant, endospore-forming bacterium (strain A121(T)) was isolated from a permafrost sample collected in the Canadian High Arctic. Phylogenetic analysis of the 16S rRNA gene sequence of strain A121(T) showed its affiliation with the group of psychrophilic and psychrotolerant members of cluster I of the genus Clostridium, Clostridium bowmanii DSM 14206(T) being the closest relative (sequence similarity 98.5 %). Levels of DNA-DNA relatedness between strain A121(T) and the type strains of phylogenetically related species ranged from 33 to 52 %. Strain A121(T) grew in PY broth at temperatures between 4 and 28 degrees C (optimum 15-20 degrees C), at pH 6.0-8.0 (optimum pH 6.5-7.2) and in NaCl concentrations of 0-10.0 % (optimum 0-2.0 %). The strain utilized a narrow range of carbohydrates as sources of carbon and energy, including glucose, fructose, trehalose, maltose and starch; it also hydrolysed gelatin. Predominant fatty acids were C(16 : 1) cis9, C(16 : 1) cis9 DMA, C(16 : 0) and C(14 : 0). The DNA G+C content was 31.5 mol%. On the basis of its overall genotypic and phenotypic characteristics, strain A121(T) is classified within a novel species of the genus Clostridium, Clostridium tagluense sp. nov. The type strain is A121(T) (=VKM B-2369(T) =DSM 17763(T)).
authors.affiliation: Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Pushchino 142290, Moscow Region, Russian Federation. suetin@ibpm.pushchino.ru
authors.forename: Stanislav V
authors.initials: SV
authors.lastname: Suetin
authors.forename: Viktoria A
authors.initials: VA
authors.lastname: Shcherbakova
authors.forename: Natalya A
authors.initials: NA
authors.lastname: Chuvilskaya
authors.forename: Elisaveta M
authors.initials: EM
authors.lastname: Rivkina
authors.forename: Natalya E
authors.initials: NE
authors.lastname: Suzina
authors.forename: Anatoliy M
authors.initials: AM
authors.lastname: Lysenko
authors.forename: David A
authors.initials: DA
authors.lastname: Gilichinsky
mesh_terms: D000693:Anaerobiosis; D001110:Arctic Regions; D015373:Bacterial Typing Techniques; D002170:Canada; D003013:Clostridium / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology*; D003080:Cold Temperature; D004269:DNA, Bacterial / Q000032:analysis; D004275:DNA, Ribosomal / Q000032:analysis; D005227:Fatty Acids / Q000032:analysis; D005838:Genotype; D046448:Ice Cover / Q000382:microbiology*; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010641:Phenotype; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods; D012150:Polymorphism, Restriction Fragment Length; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013045:Species Specificity; D013171:Spores, Bacterial / Q000502:physiology
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S