Study on the antidiabetic mechanism of a shark liver peptide, S-8300, in alloxan-induced diabetes.
OBJECTIVE
The aim was to evaluate the antidiabetic mechanism of S-8300 in alloxan-diabetic mice.


METHODS
Diabetes was induced by a single intravenous injection of alloxan (60 mg/kg). The effects of S-8300 on diabetic mice were investigated by observing the change in fasting plasma glucose, detecting Fas mRNA by reverse transcriptase-polymerase chain reaction, Fas protein expression in the pancreas by immunohistochemistry and Western blot, and the DNA fragmentation pattern forming a ladder by electrophoresis.


RESULTS
A significant decrease in fasting plasma glucose was observed, and Fas mRNA and Fas protein expression in the pancreas were attenuated in diabetic mice treated with S-8300. Treatment with S-8300 also attenuated DNA ladder formation.


CONCLUSIONS
The results suggest that S-8300 inhibits Fas protein-mediated apoptosis of pancreas cells.
Huang
Fengjie
F
School of life Science and Technology, China Pharmaceutical University, Nanjing, China.
Wu
Wutong
W
D000496:Alloxan; D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D001786:Blood Glucose / Q000378:metabolism; D003921:Diabetes Mellitus, Experimental / Q000097:blood / Q000188:drug therapy*; D004305:Dose-Response Relationship, Drug; D005260:Female; D007004:Hypoglycemic Agents / Q000494:pharmacology / Q000627:therapeutic use*; D008110:Liver Extracts / Q000494:pharmacology / Q000627:therapeutic use*; D051379:Mice; D008813:Mice, Inbred ICR; D010179:Pancreas / Q000187:drug effects / Q000473:pathology; D010455:Peptides / Q000494:pharmacology / Q000627:therapeutic use*; D012754:Sharks
D001786:Blood Glucose; D007004:Hypoglycemic Agents; D008110:Liver Extracts; D010455:Peptides; C510807:peptide S-8300; D000496:Alloxan