[The influence of glaucoma filtration surgery on anterior chamber associated immune deviation in mice].
OBJECTIVE
To study glaucoma infiltration surgery induced the anterior chamber associated immune deviation (ACAID) by with or without anterior chamber (AC) implantation of foreign epithelium removed corneal graft in mice.


METHODS
In this study, fifty Wistar mice were randomly divided into five groups (ten in each group) and additional five SD and one Wistar mouse were used as the providers of epithelium removed corneal grafts to induce ACAID as follows: Group A: corneal grafts from Wistar mouse were implanted into the AC 1 week after the infiltration surgery. Group B: the spleen cells were injected into the nape of neck 1 week after the glaucoma infiltration surgery. In group C, D, and E, the corneal grafts from SD mice were implanted into the AC at 1 week, 4 week, and 8 week after the glaucoma infiltration surgery, respectively. To induce the delayed type hypersensitivity (DTH), spleen cells were injected into the right ear pinna 1 week after the neck injection in group B, 2 weeks after the AC implantation of corneal grafts in other groups. At the same time points as the induction of DTH, heart blood was collected to detect the concentration of IL-4 and IL-10. The spleens were removed to evaluate the expression of the GATA-3 mRNA by RT-PCR. The eyeballs were enucleated and used to evaluate the histopathological changes.


RESULTS
(1) The DTH were found in group B, C, D, and E with corneal grafts of SD, but not in group A with that of Wistar. (2) The serum concentrations of IL-4 and IL-10 were statistically different in group C, D, and E (F = 49.124, 6.336; P < 0.01). The concentrations of IL-4 and IL-10 were significantly (P = 0.002) lower in group D than those in group C and E (3.759 +/- 0.250 vs 5.916 +/- 0.500 or 4.566 +/- 0.518, and 17.170 +/- 3.943 vs 44.447 +/- 17.167 or 35.643 +/- 21.233 microg/L, n = 10, respectively), which showed a biphase response fluctuation of ILs. (3) The GATA-3 mRNA was obviously up-regulated in group D and E. (4) The exudation and a few inflammatory cells in the AC were observed in group C, but not in group D and E.


CONCLUSIONS
The eye with the glaucoma infiltration surgery results in a temporary incapable of inducing ACAID, which is gradually recovered following the diminish of intraocular inflammation and the increase of levels of IL4, IL-10 and GATA-3 mRNA.
Xu
Jian-Jiang
JJ
Department of Ophthalmology, Eye & ENT Hospital, Fudan University, Shanghai 200031, China.
Sun
Xing-Huai
XH
D000818:Animals; D000867:Anterior Chamber / Q000276:immunology* / Q000473:pathology; D016039:Corneal Transplantation; D018463:Filtering Surgery; D050990:GATA3 Transcription Factor / Q000378:metabolism; D006968:Hypersensitivity, Delayed / Q000209:etiology*; D016753:Interleukin-10 / Q000378:metabolism; D015847:Interleukin-4 / Q000378:metabolism; D011184:Postoperative Period; D051381:Rats; D017207:Rats, Sprague-Dawley; D017208:Rats, Wistar; D012590:Sclera / Q000601:surgery; D013154:Spleen / Q000276:immunology / Q000378:metabolism*
D050990:GATA3 Transcription Factor; D016753:Interleukin-10; D015847:Interleukin-4