Molecular cloning, characterization and three-dimensional modeling of porcine nectin-2/CD112.
Nectin-2, also known as poliovirus receptor-related 2 or CD112, has been identified in many animals and plays a crucial role in cell recognition and adhesion. Here we report the identification of two porcine Nectin-2 (poNectin-2) isoforms. The open reading frame (ORF) of 1440 nucleotides (nt) of poNectin-2alpha encodes 479 amino acids (aa). poNectin-2delta gene consists of 1620nt of ORF with 539aa. The deduced aa sequences of poNectin-2alpha and poNectin-2delta gene exhibit high identity with human (74% and 79%) and mouse (71% and 76%) orthologs, respectively. The ectodomains of deduced poNectin-2 protein share the structural feature of mammalian Nectin-2, including a conserved cysteine skeleton important for the formation of the three-dimensional structure. RT-PCR analysis showed that the mRNA of both poNectin-2 isoforms was broadly expressed in various tissues and cells. poNectin-2 gene was mapped to chromosome 6q21. Information from this study will help to elucidate the Nectin-2 pathway in xenotransplant immunity.
Wang
Lu
L
Institute of Organ Transplantation, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, PR China.
Zhang
Wei
W
Wu
Ding-An
DA
Chen
Chao
C
Xu
Qin-Zi
QZ
Zhao
Bo
B
Liao
Yong-De
YD
Zhao
Shu-Hong
SH
Fu
Xiang-Ning
XN
Chen
Shi
S
Zhu
Min
M
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015815:Cell Adhesion Molecules / Q000737:chemistry* / Q000235:genetics*; D002460:Cell Line; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D006801:Humans; D008297:Male; D051379:Mice; D008958:Models, Molecular; D008969:Molecular Sequence Data; D000075983:Nectins; D010802:Phylogeny; D017434:Protein Structure, Tertiary; D017386:Sequence Homology, Amino Acid; D040681:Structural Homology, Protein; D034421:Sus scrofa; D013552:Swine / Q000235:genetics* / Q000276:immunology*
D015815:Cell Adhesion Molecules; D018076:DNA, Complementary; C000619016:Nectin2 protein, mouse; D000075983:Nectins