Inhibiting gastric cancer-associated angiogenesis by CIAPIN1 siRNA.
Angiogenesis plays an essential role in tumor growth and metastasis and is a promising target for cancer therapy. We characterized the effects of selective CIAPIN1 inhibition on the angiogenesis gastric cancer cell line SGC7901 by stable transfection of CIAPIN1 siRNA. Our study has been shown that CIAPIN1 play the determined role in tumor growth and multidrug resistance. The conditioned media obtained from SGC7901 treated with CIAPIN1 siRNA suppressed in vitro the proliferation, migration and tube formation of human umbilical vein endothelial cells compared with untransfected cells or cells transfected with control vector alone. Furthermore, the stable transfection of CIAPIN1 siRNA inhibited in vivo tumorigenicity and angiogenesis. Our findings support that selective inhibition of CIAPIN1 alone plays an instrumental role on gastric cancer associated angiogenesis.
Yan
Kun
K
Department of General Surgery, Second Affiliated Hospital, Xi'an Jiaotong University, Xi'an, China.
He
Li-Jie
LJ
Cheng
Wei
W
Ji
Zong-Zheng
ZZ
Zhao
Bin-Xiu
BX
Hui
Xiao-Li
XL
Cao
Shan-Shan
SS
Chen
Bei
B
He
Li
L
Liang
Shu-Hui
SH
Miao
Yv
Y
D000818:Animals; D014408:Biomarkers, Tumor / Q000032:analysis; D015153:Blotting, Western; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D018432:Drug Resistance, Multiple; D042783:Endothelial Cells / Q000502:physiology; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins / Q000037:antagonists & inhibitors* / Q000235:genetics* / Q000378:metabolism; D051379:Mice; D008819:Mice, Nude; D009389:Neovascularization, Pathologic / Q000235:genetics; D034741:RNA, Small Interfering / Q000235:genetics* / Q000378:metabolism; D013274:Stomach Neoplasms / Q000098:blood supply* / Q000235:genetics* / Q000378:metabolism; D014162:Transfection; D014471:Umbilical Veins / Q000502:physiology
D014408:Biomarkers, Tumor; C489114:CIAPIN1 protein, human; D047908:Intracellular Signaling Peptides and Proteins; D034741:RNA, Small Interfering