title: Development of an integrative database with 499 novel microsatellite markers for Macaca fascicularis.
abstract: BACKGROUND
  Cynomolgus macaques (Macaca fascicularis) are a valuable resource for linkage studies of genetic disorders, but their microsatellite markers are not sufficient. In genetic studies, a prerequisite for mapping genes is development of a genome-wide set of microsatellite markers in target organisms. A whole genome sequence and its annotation also facilitate identification of markers for causative mutations. The aim of this study is to establish hundreds of microsatellite markers and to develop an integrative cynomolgus macaque genome database with a variety of datasets including marker and gene information that will be useful for further genetic analyses in this species.
  
  
  RESULTS
  We investigated the level of polymorphisms in cynomolgus monkeys for 671 microsatellite markers that are covered by our established Bacterial Artificial Chromosome (BAC) clones. Four hundred and ninety-nine (74.4%) of the markers were found to be polymorphic using standard PCR analysis. The average number of alleles and average expected heterozygosity at these polymorphic loci in ten cynomolgus macaques were 8.20 and 0.75, respectively.
  
  
  CONCLUSIONS
  BAC clones and novel microsatellite markers were assigned to the rhesus genome sequence and linked with our cynomolgus macaque cDNA database (QFbase). Our novel microsatellite marker set and genomic database will be valuable integrative resources in analyzing genetic disorders in cynomolgus macaques.
authors.affiliation: Tsukuba Primate Research Center, National Institute of Biomedical Innovation, Tsukuba, Ibaraki, Japan. higashino@nibio.go.jp
authors.forename: Atsunori
authors.initials: A
authors.lastname: Higashino
authors.forename: Naoki
authors.initials: N
authors.lastname: Osada
authors.forename: Yumiko
authors.initials: Y
authors.lastname: Suto
authors.forename: Makoto
authors.initials: M
authors.lastname: Hirata
authors.forename: Yosuke
authors.initials: Y
authors.lastname: Kameoka
authors.forename: Ichiro
authors.initials: I
authors.lastname: Takahashi
authors.forename: Keiji
authors.initials: K
authors.lastname: Terao
mesh_terms: D000483:Alleles; D000818:Animals; D022202:Chromosomes, Artificial, Bacterial; D030541:Databases, Genetic; D005260:Female; D005819:Genetic Markers; D023281:Genomics; D006801:Humans; D008252:Macaca fascicularis / Q000235:genetics*; D008297:Male; D018895:Microsatellite Repeats; D011110:Polymorphism, Genetic; D017422:Sequence Analysis, DNA
chemical_list: D005819:Genetic Markers