[Cyto-genotoxicity induced by cigarette smoke condensates in human peripheral blood lymphocytes in vitro].
OBJECTIVE
To investigate the cyto-genotoxicity of cigarette smoke condensates (CSCs) in human peripheral blood lymphocytes with different assays in vitro.


METHODS
Human lymphocytes were exposed to particle matter of cigarette smoke combined with or without S9 mixtures at doses of 25, 50, 75, 100 and 125 microg/ml for 3 h. The cytotoxicity induced by CSCs was detected by CCK-8 assay. The DNA damage, DNA repair (repair time: 30, 60, 90, 120 and 240 min, respectively) and the somatic cell mutations induced by 75 microg/ml CSCs were measured by comet assay, hprt gene and TCR gene mutation tests, respectively.


RESULTS
CCK-8 assay indicated that the cell viability decreased with CSCs doses. At the doses of 100, 125 microg/ml, the cell viability of CSCs +S9 group was significantly higher than that of CSCs -S9 group (P < 0.05, P < 0.01). In comet assay, DNA damage significantly increased in a dose-dependent manner, as compared with controls (P < 0.01). Moreover, there was significant difference between -S9 group and +S9 group (P < 0.05, P < 0.01). The Mf-TCR at each dose group was significantly higher than that of controls (P < 0.05, P < 0.01). The Mf-hprt at high-dose groups were significantly higher than that of controls (P < 0.01), and significant difference of Mf-TCR and Mf-hprt at high doses of CSCs between -S9 group and +S9 group (P < 0.05, P < 0.01). The DNA damage induced by CSCs +S9 or CSCs -S9 could be repaired, but DNA repair speed was different between -S9 group and +S9 group (P < 0.05, P < 0.01).


CONCLUSIONS
CSCs may induce cyto-genotoxicity in human peripheral blood lymphocytes in vitro, but S9 mix could reduce the toxicity of CSCs and impact DNA repair speed.
Lou
Jian-lin
JL
Technical Center, China Tobacco Zhejiang Industrial Co., Ltd, Hangzhou 310008, China.
Zhou
Guo-jun
GJ
Chu
Guo-hai
GH
Huang
Fang-fang
FF
Jiang
Jian
J
Zheng
Shu
S
Lu
Ye-zhen
YZ
Li
Xiao-xue
XX
Chen
Zhi-jian
ZJ
He
Ji-liang
JL
D002478:Cells, Cultured; D020552:Comet Assay; D004249:DNA Damage / Q000187:drug effects*; D004260:DNA Repair / Q000187:drug effects*; D006801:Humans; D008214:Lymphocytes / Q000187:drug effects*; D008297:Male; D009154:Mutation; D014028:Tobacco Smoke Pollution / Q000009:adverse effects*; D055815:Young Adult
D014028:Tobacco Smoke Pollution