High-density integrated linkage map based on SSR markers in soybean.
A well-saturated molecular linkage map is a prerequisite for modern plant breeding. Several genetic maps have been developed for soybean with various types of molecular markers. Simple sequence repeats (SSRs) are single-locus markers with high allelic variation and are widely applicable to different genotypes. We have now mapped 1810 SSR or sequence-tagged site markers in one or more of three recombinant inbred populations of soybean (the US cultivar 'Jack' x the Japanese cultivar 'Fukuyutaka', the Chinese cultivar 'Peking' x the Japanese cultivar 'Akita', and the Japanese cultivar 'Misuzudaizu' x the Chinese breeding line 'Moshidou Gong 503') and have aligned these markers with the 20 consensus linkage groups (LGs). The total length of the integrated linkage map was 2442.9 cM, and the average number of molecular markers was 90.5 (range of 70-114) for the 20 LGs. We examined allelic diversity for 1238 of the SSR markers among 23 soybean cultivars or lines and a wild accession. The number of alleles per locus ranged from 2 to 7, with an average of 2.8. Our high-density linkage map should facilitate ongoing and future genomic research such as analysis of quantitative trait loci and positional cloning in addition to marker-assisted selection in soybean breeding.
Hwang
Tae-Young
TY
National Agricultural Research Center for Hokkaido Region, Sapporo, Hokkaido 062-8555, Japan.
Sayama
Takashi
T
Takahashi
Masakazu
M
Takada
Yoshitake
Y
Nakamoto
Yumi
Y
Funatsuki
Hideyuki
H
Hisano
Hiroshi
H
Sasamoto
Shigemi
S
Sato
Shusei
S
Tabata
Satoshi
S
Kono
Izumi
I
Hoshi
Masako
M
Hanawa
Masayoshi
M
Yano
Chizuru
C
Xia
Zhengjun
Z
Harada
Kyuya
K
Kitamura
Keisuke
K
Ishimoto
Masao
M
D002874:Chromosome Mapping; D003433:Crosses, Genetic; D018744:DNA, Plant / Q000032:analysis / Q000302:isolation & purification; D020224:Expressed Sequence Tags; D005819:Genetic Markers / Q000235:genetics; D018745:Genome, Plant; D018895:Microsatellite Repeats / Q000235:genetics*; D011110:Polymorphism, Genetic; D016324:Sequence Tagged Sites; D013025:Glycine max / Q000145:classification / Q000235:genetics*
D018744:DNA, Plant; D005819:Genetic Markers