title: In vivo nonlinear spectral imaging in mouse skin.
abstract: We report on two-photon autofluorescence and second harmonic spectral imaging of live mouse tissues. The use of a high sensitivity detector and ultraviolet optics allowed us to record razor-sharp deep-tissue spectral images of weak autofluorescence and short-wavelength second harmonic generation by mouse skin. Real-color image representation combined with depth-resolved spectral analysis enabled us to identify tissue structures. The results show that linking nonlinear deep-tissue imaging microscopy with autofluorescence spectroscopy has the potential to provide important information for the diagnosis of skin tissues.
authors.forename: Jonathan A
authors.initials: JA
authors.lastname: Palero
authors.forename: H S
authors.initials: HS
authors.lastname: de Bruijn
authors.forename: A
authors.initials: A
authors.lastname: van der Ploeg-van den Heuvel
authors.forename: H J C M
authors.initials: HJ
authors.lastname: Sterenborg
authors.forename: H C
authors.initials: HC
authors.lastname: Gerritsen