Application of whole mouse embryo culture system on stem cell research.
The normal development of mouse embryo in vivo could be maintained in vitro up to 72 h in the presence of rat serum which is continuously supplied with the appropriate concentration of O(2) and CO(2). There are several applications of the whole mouse embryo culture model for study of cellular dynamics in hematopoiesis and its interaction with vasculogenesis. In this protocol, we have described details of manipulation techniques in combination with the whole embryo culture and also some advance techniques applied to the mouse embryo such as intra-cardiac inoculation of acetylated low density lipoprotein for cell-specific labeling and engraftment of donor yolk-sac from different genotype/phenotype mouse embryo onto the yolk-sac of host mouse for study of the dynamic distribution of hematopoietic cell.
Kulkeaw
Kasem
K
Faculty of Medical Sciences, Department of Hematopoietic Stem Cells, SSP Stem Cell Unit, Kyushu University, Fukuoka, Japan.
Mizuochi
Chiyo
C
Horio
Yuka
Y
Osumi
Noriko
N
Tsuji
Kohichiro
K
Sugiyama
Daisuke
D
D000818:Animals; D046149:Embryo Culture Techniques / Q000295:instrumentation* / Q000379:methods*; D033041:Embryo Research; D051379:Mice; D008810:Mice, Inbred C57BL; D013234:Stem Cells