Differential requirement of ribosomal protein S6 by plant RNA viruses with different translation initiation strategies.
Potyvirus infection has been reported to cause an increase in the mRNA transcripts of many plant ribosomal proteins (r-proteins). In this study, increased expression of r-protein mRNA transcripts was determined to occur in Nicotiana benthamiana during infection by potyviruses as well as a tobamovirus demonstrating that this response is not unique to potyviruses. Five r-protein genes, RPS6, RPL19, RPL13, RPL7, and RPS2, were silenced in N. benthamiana to test their roles in viral infection. The accumulation of both Turnip mosaic virus (TuMV), a potyvirus, and Tobacco mosaic virus (TMV), a tobamovirus, was dependent on RPL19, RPL13, RPL7, and RPS2. However, TMV was able to accumulate in RPS6-silenced plants while accumulation of TuMV and Tomato bushy stunt virus (TBSV) was abolished. These results demonstrate that cap-independent TuMV and TBSV require RPS6 for their accumulation, whereas accumulation of TMV is independent of RPS6.
Yang
Chunling
C
Department of Plant Pathology, Iowa State University, 351 Bessey Hall, Ames, IA 50011-1020, USA.
Zhang
Chunquan
C
Dittman
Jaime D
JD
Whitham
Steven A
SA
D020868:Gene Silencing; D010442:Peptide Chain Initiation, Translational; D017800:Potyvirus / Q000254:growth & development / Q000502:physiology*; D038601:Ribosomal Protein S6 / Q000096:biosynthesis*; D014026:Nicotiana / Q000821:virology*; D017898:Tobamovirus / Q000254:growth & development / Q000502:physiology*
D038601:Ribosomal Protein S6