Interactions of MAP/microtubule affinity regulating kinases with the adaptor complex AP-2 of clathrin-coated vesicles.
MARK [microtubule-associated proteins (MAPs)/microtubule affinity regulating kinase]/Par-1 (partition defective) phosphorylate MAPs tau, MAP2 and MAP4 at KXGS motifs and thereby regulate microtubule dynamics and transport processes in cells [Drewes et al., Cell1997;89:297-308]. We report here that MARK copurifies with clathrin-coated vesicles (CCVs) via interaction with the adaptor complex AP-2. The adaptin binding site on MARK includes the regulatory loop of its catalytic domain. Immunofluorescence demonstrates the colocalization of MARK with AP-2 and clathrin, as well as other MARK-interacting proteins such as PAK5. The results are consistent with an observed influence of MARK on the trafficking of CCVs. Cell Motil. Cytoskeleton 2009. (c) 2009 Wiley-Liss, Inc.
Schmitt-Ulms
Gerold
G
Centre for Research in Neurodegenerative Disease, University of Toronto, Toronto, Canada.
Matenia
Dorthe
D
Drewes
Gerard
G
Mandelkow
Eva-Maria
EM
D033962:Adaptor Protein Complex 2 / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D001665:Binding Sites; D016466:CHO Cells; D022163:Clathrin-Coated Vesicles / Q000378:metabolism*; D006224:Cricetinae; D003412:Cricetulus; D005455:Fluorescent Antibody Technique; D008856:Microscopy, Fluorescence; D008869:Microtubule-Associated Proteins / Q000235:genetics / Q000378:metabolism*; D008870:Microtubules / Q000378:metabolism*; D020928:Mitogen-Activated Protein Kinases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D010766:Phosphorylation; D011485:Protein Binding; D017433:Protein Structure, Secondary; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D013552:Swine; D054462:p21-Activated Kinases / Q000235:genetics / Q000378:metabolism
D033962:Adaptor Protein Complex 2; D008869:Microtubule-Associated Proteins; D011994:Recombinant Proteins; D054462:p21-Activated Kinases; D020928:Mitogen-Activated Protein Kinases