Soy lecithin replaces egg yolk for cryopreservation of human sperm without adversely affecting postthaw motility, morphology, sperm DNA integrity, or sperm binding to hyaluronate.
Semen specimens (one ejaculate from each of 20 consenting study participants) were subjected to routine semen analysis, an in vitro sperm binding assay (HBA), and a sperm chromatin dispersion assay (HaloSperm), both before and after cryopreservation using cryoprotectant media supplemented with either egg yolk or soy lecithin. Comparing the equivalency of the two phospholipid cryopreservation supplements with regard to postthaw functional parameters demonstrated that there were no statistically significant differences between the two supplements for [1] recovery of motile sperm, [2] maintenance of sperm cell morphology, [3] maintenance of the ability of sperm to bind to hyaluronate in vitro, or [4] maintenance of sperm DNA integrity.
Reed
Michael L
ML
Center for Reproductive Medicine of New Mexico, Albuquerque, New Mexico 87106, USA. mleroyreed@yahoo.com
Ezeh
Peace C
PC
Hamic
Amanda
A
Thompson
Douglas J
DJ
Caperton
Charles L
CL
D015925:Cryopreservation / Q000379:methods; D003451:Cryoprotective Agents / Q000737:chemistry / Q000494:pharmacology; D004247:DNA / Q000737:chemistry / Q000187:drug effects; D053938:DNA Fragmentation / Q000187:drug effects; D004527:Egg Proteins / Q000494:pharmacology*; D005240:Feasibility Studies; D006801:Humans; D006820:Hyaluronic Acid / Q000378:metabolism*; D054709:Lecithins / Q000494:pharmacology*; D008297:Male; D012662:Semen Preservation / Q000379:methods*; D030262:Soybean Proteins / Q000737:chemistry / Q000494:pharmacology*; D013081:Sperm Motility / Q000187:drug effects; D013094:Spermatozoa / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism / Q000502:physiology
D003451:Cryoprotective Agents; D004527:Egg Proteins; D054709:Lecithins; D030262:Soybean Proteins; D006820:Hyaluronic Acid; D004247:DNA