Novel system for storage of buffy-coat-derived platelet concentrates in a glucose-based platelet additive solution: parameters and metabolism during storage and comparison to plasma.
BACKGROUND
In Europe, buffy-coat processing allows for the use of platelet additive solutions (PAS). These solutions, however, have long been questioned for their lack of glucose, a potentially essential nutrient for platelet storage. Using a novel, practical, two-part system for incorporation of glucose into an additive solution (PAS-G), this study compares platelet storage in plasma to storage in PAS-G.


METHODS
A paired study design of platelet concentrates (PC) were prepared from leucoreduced pools of eight buffy coats (BCP) split into two equal pools, with suspension in autologous plasma, or PAS-G. On days 2, 5, 7 and 9 of storage, samples were tested using standard in vitro platelet parameters. Data were analysed by paired Student's t-tests.


RESULTS
During storage, PCs in PAS-G maintain a quality profile that is strikingly similar to PCs stored in plasma in terms of platelet activation (CD62) morphology score, swirl, glucose metabolism and pH. However, PCs in PAS-G perform lower (P < 0.05) in the %ESC and %HSR assays.


CONCLUSIONS
PAS-G's novel presentation allows incorporation of glucose into the additive solution so that it is roughly equivalent to plasma for the maintenance of buffy-coat PCs.
Gyongyossy-Issa
M I C
MI
Canadian Blood Services, UBC Center for Blood Research, University of British Columbia, 2350 Health Sciences Mall, Vancouver, BC, Canada. missa@interchange.ubc.ca
Zhang
J G
JG
Culibrk
B
B
Hunter
F
F
Levin
E
E
Scammell
K
K
Weiss
S
S
Holmes
D L
DL
Holme
S
S
D001792:Blood Platelets / Q000166:cytology* / Q000378:metabolism; D001793:Blood Preservation / Q000379:methods*; D005060:Europe; D005947:Glucose / Q000378:metabolism*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D047589:Leukocyte Reduction Procedures; D019999:Pharmaceutical Solutions / Q000737:chemistry; D010949:Plasma; D015539:Platelet Activation
D019999:Pharmaceutical Solutions; D005947:Glucose