title: The mycobacterial glycolipid glucose monomycolate induces a memory T cell response comparable to a model protein antigen and no B cell response upon experimental vaccination of cattle.
abstract: Glycolipids are presented to T cells by human group 1 CD1 proteins, but are not used as subunit vaccines yet. Experimental immunizations with pure mycobacterial glucose monomycolate (GMM) and keyhole limpet haemocyanin (KLH) in cattle, a species which, unlike mice, expresses group 1 CD1, showed that GMM was equally efficient as KLH in generating T cell responses in blood, but not in the draining lymph node. Also, KLH induced strong antibody responses whereas GMM did not. These data suggest that non-overlapping T cell populations are targeted and demonstrate the potential of glycolipids as a special class of subunit vaccine candidates.
authors.affiliation: Department of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, Utrecht University, Yalelaan 1, Utrecht, The Netherlands.
authors.forename: Thi Kim Anh
authors.initials: TK
authors.lastname: Nguyen
authors.forename: Ad P
authors.initials: AP
authors.lastname: Koets
authors.forename: Wiebren J
authors.initials: WJ
authors.lastname: Santema
authors.forename: Willem
authors.initials: W
authors.lastname: van Eden
authors.forename: Victor P M G
authors.initials: VP
authors.lastname: Rutten
authors.forename: Ildiko
authors.initials: I
authors.lastname: Van Rhijn
mesh_terms: D000276:Adjuvants, Immunologic / Q000008:administration & dosage / Q000494:pharmacology; D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology; D002417:Cattle; D006017:Glycolipids / Q000276:immunology* / Q000302:isolation & purification; D006433:Hemocyanins / Q000276:immunology; D007156:Immunologic Memory; D009163:Mycobacterium bovis / Q000737:chemistry* / Q000276:immunology; D000644:Quaternary Ammonium Compounds / Q000008:administration & dosage / Q000494:pharmacology; D013601:T-Lymphocytes / Q000276:immunology*; D032581:Tuberculosis Vaccines / Q000276:immunology*
chemical_list: D000276:Adjuvants, Immunologic; D000907:Antibodies, Bacterial; D006017:Glycolipids; D000644:Quaternary Ammonium Compounds; D032581:Tuberculosis Vaccines; C048938:glucose mycolate; C015831:dimethyldioctadecylammonium; D006433:Hemocyanins; C032808:keyhole-limpet hemocyanin