Arginine vasopressin antinociception in the rat nucleus raphe magnus is involved in the endogenous opiate peptide and serotonin system.
Arginine vasopressin (AVP) in the nucleus raphe magnus (NRM) has been implicated in antinociception. This communication was designed to investigate which neuropeptide and neurotransmitter are involved in AVP antinociception in the rat NRM. The results showed that (1) in the NRM perfuse liquid, pain stimulation could increase the concentrations of AVP, leucine-enkephalin (L-Ek), methionine-enkephalin (M-Ek), beta-endorphin (beta-Ep), serotonin (5-HT) and 5-hydroxyindoleacetic acid (5-HIAA), but not change the concentrations of dynorphinA(1-13) (DynA(1-13)), oxytocin, achetylcholine, choline, gamma-aminobutyric acid, glutamate, dopamine, 3,4-dihydroxyphenylacetic acid, homovanilic acid, norepinephrine and epinephrine; (2) in the NRM perfuse liquid, AVP increased the concentrations of L-Ek, M-Ek, beta-Ep, DynA(1-13), 5-HT and 5-HIAA, but did not change the concentrations of oxytocin and the other studied neurotransmitters; (3) AVP antinociception in the NRM was attenuated by cypoheptadine (a 5-HT-receptor antagonist) or naloxone (an opiate receptor antagonist), but was not influenced by the other studied receptor antagonists. The data suggested that AVP antinociception in the NRM might be involved in endogenous opiate peptide and 5-HT system.
Yang
Jun
J
State Key Laboratory of New Technology for Pharmaceuticals, Jiangsu Provincial Institute for Novel Pharmaceuticals at Taizhou, Yangtze River Pharmaceutical Group, Taizhou, Jiangsu 225321, China. yj6676@yahoo.com
Yuan
Huifeng
H
Chu
Jiegen
J
Yang
Yu
Y
Xu
Hongtao
H
Wang
Gen
G
Liu
Wen-Yan
WY
Lin
Bao-Cheng
BC
D000700:Analgesics / Q000378:metabolism*; D000818:Animals; D001127:Arginine Vasopressin / Q000378:metabolism*; D002794:Choline / Q000378:metabolism; D004743:Enkephalin, Leucine / Q000378:metabolism; D004744:Enkephalin, Methionine / Q000378:metabolism; D018698:Glutamic Acid / Q000378:metabolism; D007211:Indoles / Q000378:metabolism; D008297:Male; D018847:Opioid Peptides / Q000378:metabolism*; D010121:Oxytocin / Q000378:metabolism; D010146:Pain / Q000188:drug therapy; D010147:Pain Measurement; D011903:Raphe Nuclei / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012701:Serotonin / Q000378:metabolism*; D005680:gamma-Aminobutyric Acid / Q000378:metabolism
D000700:Analgesics; D007211:Indoles; D018847:Opioid Peptides; D001127:Arginine Vasopressin; D012701:Serotonin; D018698:Glutamic Acid; D010121:Oxytocin; D005680:gamma-Aminobutyric Acid; D004744:Enkephalin, Methionine; D004743:Enkephalin, Leucine; C018079:5-(hydroxy-3-indolyl)lactic acid; D002794:Choline