Distinct origins and genetic programs of head muscle satellite cells.
Adult skeletal muscle possesses a remarkable regenerative capacity, due to the presence of satellite cells, adult muscle stem cells. We used fate-mapping techniques in avian and mouse models to show that trunk (Pax3(+)) and cranial (MesP1(+)) skeletal muscle and satellite cells derive from separate genetic lineages. Similar lineage heterogeneity is seen within the head musculature and satellite cells, due to their shared, heterogenic embryonic origins. Lineage tracing experiments with Isl1Cre mice demonstrated the robust contribution of Isl1(+) cells to distinct jaw muscle-derived satellite cells. Transplantation of myofiber-associated, Isl1-derived satellite cells into damaged limb muscle contributed to muscle regeneration. In vitro experiments demonstrated the cardiogenic nature of cranial- but not trunk-derived satellite cells. Finally, overexpression of Isl1 in the branchiomeric muscles of chick embryos inhibited skeletal muscle differentiation in vitro and in vivo, suggesting that this gene plays a role in the specification of cardiovascular and skeletal muscle stem cell progenitors.
Harel
Itamar
I
Department of Biological Regulation, Weizmann Institute of Science, Rehovot, Israel.
Nathan
Elisha
E
Tirosh-Finkel
Libbat
L
Zigdon
Hila
H
Guimarães-Camboa
Nuno
N
Evans
Sylvia M
SM
Tzahor
Eldad
E
D000818:Animals; D051792:Basic Helix-Loop-Helix Proteins / Q000378:metabolism; D002454:Cell Differentiation; D019070:Cell Lineage; D002478:Cells, Cultured; D002642:Chick Embryo; D004622:Embryo, Mammalian / Q000166:cytology / Q000378:metabolism; D004625:Embryo, Nonmammalian / Q000166:cytology / Q000378:metabolism; D005121:Extremities / Q000473:pathology; D018507:Gene Expression Regulation, Developmental; D018740:Genetic Heterogeneity; D006257:Head; D018398:Homeodomain Proteins / Q000378:metabolism; D060850:LIM-Homeodomain Proteins; D008648:Mesoderm / Q000166:cytology / Q000637:transplantation; D051379:Mice; D024510:Muscle Development; D009132:Muscles / Q000473:pathology / Q000502:physiology; D009206:Myocardium / Q000378:metabolism; D000072041:PAX3 Transcription Factor; D051761:Paired Box Transcription Factors / Q000378:metabolism; D011784:Quail / Q000196:embryology; D012038:Regeneration; D032496:Satellite Cells, Skeletal Muscle / Q000166:cytology* / Q000378:metabolism*; D014157:Transcription Factors
D051792:Basic Helix-Loop-Helix Proteins; D018398:Homeodomain Proteins; D060850:LIM-Homeodomain Proteins; D000072041:PAX3 Transcription Factor; D051761:Paired Box Transcription Factors; D014157:Transcription Factors; C101538:Mesp1 protein, mouse; C063843:insulin gene enhancer binding protein Isl-1; C068686:Pax3 protein, mouse