title: Phylogenetic composition and properties of bacteria coexisting with the fungus Hypholoma fasciculare in decaying wood.
abstract: White-rot fungi are major degraders of woody materials in terrestrial environments because of their ability to decompose lignin. However, little is known on the possible associations of white-rot fungi with other microorganisms during wood decay. We investigated the numbers, community composition and functional traits of bacteria present in natural wood samples under advanced decay by the white-rot basidiomycete Hypholoma fasciculare. The wood samples contained high numbers of cultivable bacteria (0.2-8 x 10(9) colony forming units (CFU) per g of dry wood). Most cultivable bacteria belonged to Proteobacteria and Acidobacteria (75% and 23% of sequences, respectively). The same phyla were also found to be dominant (59% and 23%, respectively) using a non-culturable quantification technique, namely, direct cloning and sequencing of 16sRNA genes extracted from wood. Bacteria that could be subcultured consisted of acid-tolerant strains that seemed to rely on substrates released by lignocellulolytic enzyme activities of the fungus. There were no indications for antagonism (antibiosis) of the bacteria against the fungus.
authors.affiliation: Laboratory of Environmental Microbiology, Institute of Microbiology of the ASCR, v.v.i., Prague 4 14220, Czech Republic.
authors.forename: Vendula
authors.initials: V
authors.lastname: Valásková
authors.forename: Wietse
authors.initials: W
authors.lastname: de Boer
authors.forename: Paulien J A Klein
authors.initials: PJ
authors.lastname: Gunnewiek
authors.forename: Martin
authors.initials: M
authors.lastname: Pospísek
authors.forename: Petr
authors.initials: P
authors.lastname: Baldrian
mesh_terms: D000363:Agaricales / Q000254:growth & development*; D001419:Bacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D014934:Wood / Q000382:microbiology*
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S