Multiple component isolation in preparative multidimensional gas chromatography with characterisation by mass spectrometry and nuclear magnetic resonance spectroscopy.
The preparative scale isolation of multiple components from an essential oil matrix is described using multidimensional gas chromatography (prep-MDGC) which allows their further characterisation by mass spectrometry and nuclear magnetic resonance (NMR) spectroscopy. Menthol, linalyl acetate, carvone and geraniol were isolated individually, and were also collected in various combinations. It was demonstrated to be possible to collect multiple selected components from numerous repeat injections of the sample, to permit increased mass recovery from an external cryotrap collection device. Peak retention times remained reproducible (<0.3s) over the repeated injections and switching events. This methodology may be utilised to confirm peak identity or to produce unique mixed-component reference standards, for instance to allow their identification in other samples using GC/MS, or identify them in comprehensive two-dimensional gas chromatography (GCxGC) analysis.
Rühle
Christian
C
Australian Centre for Research on Separation Science, School of Applied Sciences, RMIT University, Melbourne 3001, Australia.
Eyres
Graham T
GT
Urban
Sylvia
S
Dufour
Jean-Pierre
JP
Morrison
Paul D
PD
Marriott
Philip J
PJ
D002849:Chromatography, Gas / Q000379:methods*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D013058:Mass Spectrometry / Q000379:methods*; D009822:Oils, Volatile / Q000737:chemistry* / Q000302:isolation & purification*; D010938:Plant Oils / Q000737:chemistry* / Q000302:isolation & purification*
D009822:Oils, Volatile; D010938:Plant Oils