Hepatitis B virus genetic typing using mass-spectrometry.
Mini-sequencing with subsequent result registration using MALDI-ToF mass-spectrometry was employed for hepatitis B virus genetic typing in Russian population. This approach was employed for hepatitis B virus genetic typing in HBsAg-positive patients with chronic hepatitis B, hepatitis of combined etiology and hepatic cirrhosis and allowed to show the prevalence of D genotype (83.3%) in all groups of patients. Other hepatitis B virus genotypes: genotype A (5.9%), genotype C (3.6%), and mixed infection with D and C (7.2%) were also found in patients with chronic hepatitis B and hepatic cirrhosis. All genotypes were found in patients with chronic hepatitis B and hepatic cirrhosis. Chronic hepatitis of combined etiology was noted only in patients with genotype D. Possibility of detection of mixed infection with hepatitis B viruses of various genotypes is a distinct advantage of mini-sequencing approach over direct nucleotide sequence evaluation for hepatitis B virus genetic typing.
Malakhova
M V
MV
Scientific Research Institute of Physical-Chemical Medicine, Moscow State Medical-Stomatological University, Moscow, Russia. maja_m@mail.ru
Ilina
E N
EN
Govorun
V M
VM
Shutko
S A
SA
Dudina
K R
KR
Znoyko
O O
OO
Klimova
E A
EA
Iushchuk
N D
ND
D001483:Base Sequence; D005838:Genotype; D006509:Hepatitis B / Q000821:virology; D006515:Hepatitis B virus / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D008828:Microbiological Techniques / Q000379:methods*; D008969:Molecular Sequence Data; D012689:Sequence Homology, Nucleic Acid; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization