Endocrine-disrupting equivalents in industrial effluents discharged into Yangtze River.
The endocrine-disrupting equivalents in effluents from three chemical industry wastewater treatment systems in the vicinity of Yangtze River were determined by several transactivation reporter gene assays. Transient transfections of African green monkey kidney cell line (CV-1) were used to determine the estrogenic, anti-androgenic and anti-thyroid equivalents in the effluents. Organic extracts of the effluents contained compounds that were potent anti-androgens and the activities measured as an equivalent concentration of flutamide were 45.53, 34.65 and 91.61 nM, respectively. The extracts also contained detectable concentrations of thyroid antagonists. Estrogenic activities, measured with the reporter gene assay, were near or below the method detection limit (0.58 pM as E2). Concentrations of some of the major constituents such as di(2-ethylhexyl)phthalate, dibutyl phthalate, 2,6-dinitrotoluene and nitrobenzene were quantified. The data suggest that the reporter gene assay is useful to predication of endocrine disrupting effects in polluted aquatic body.
Shi
Wei
W
State Key Laboratory of Pollution Control and Resource Reuse & School of the Environment, Nanjing University, 210093 Nanjing, People's Republic of China.
Wang
Xiaoyi
X
Hu
Wei
W
Sun
Hong
H
Shen
Ouxi
O
Liu
Hongling
H
Wang
Xinru
X
Giesy
John P
JP
Cheng
Shupei
S
Yu
Hongxia
H
D000818:Animals; D002460:Cell Line; D002681:China; D002522:Chlorocebus aethiops; D052244:Endocrine Disruptors / Q000032:analysis* / Q000633:toxicity*; D004784:Environmental Monitoring; D017930:Genes, Reporter; D007220:Industrial Waste / Q000032:analysis*; D008156:Luciferases / Q000235:genetics; D011944:Receptors, Androgen / Q000235:genetics; D011960:Receptors, Estrogen / Q000235:genetics; D011988:Receptors, Thyroid Hormone / Q000235:genetics; D045483:Rivers / Q000737:chemistry*; D015533:Transcriptional Activation; D014162:Transfection; D014874:Water Pollutants, Chemical / Q000032:analysis* / Q000633:toxicity*
D052244:Endocrine Disruptors; D007220:Industrial Waste; D011944:Receptors, Androgen; D011960:Receptors, Estrogen; D011988:Receptors, Thyroid Hormone; D014874:Water Pollutants, Chemical; D008156:Luciferases