title: Synthetic oligoribonucleotides-containing secondary structures act as agonists of Toll-like receptors 7 and 8.
abstract: Single-stranded RNAs act as ligands of Toll-like receptors (TLRs) 7 and 8 and induce immune responses. In the present study, we have designed and synthesized phosphorothioate oligoribonucleotides (ORNs) with self-complementary sequences that form duplex structures with either 3'- or 5'-overhanging sequences. We studied the new ORNs for their duplex formation, nuclease stability, and ability to induce immune-stimulatory activate through TLR7 and TLR8 in TLR-transfected cell lines, human PBMCs, human pDCs, and in vivo in mice. Thermal melting and gel electrophoresis studies showed that all ORNs formed secondary structures and that the thermal stability of the duplex is depended on the length and GC composition of the duplex. Nuclease stability of ORNs increased with increasing thermal stability of the duplex formed. All ORN showed TLR8 activity in HEK293 cells, and induced cytokine and chemokine production in human PBMC cultures. In addition to TLR8 activity, two ORNs containing a 'CUGAAUU' motif in the duplex-forming region induced immune stimulation through TLR7 in HEK293 cells, human PBMC and pDC cultures, and in vivo in mice. These results suggest that secondary structures in ORN provide nuclease stability and lead to stimulation of immune responses through TLR8 as well as TLR7 depending on the presence of specific nucleotide motifs.
authors.affiliation: Idera Pharmaceuticals, Inc., 167 Sidney Street, Cambridge, MA 02139, USA.
authors.forename: Tao
authors.initials: T
authors.lastname: Lan
authors.forename: Mallikarjuna Reddy
authors.initials: MR
authors.lastname: Putta
authors.forename: Daqing
authors.initials: D
authors.lastname: Wang
authors.forename: Meiru
authors.initials: M
authors.lastname: Dai
authors.forename: Dong
authors.initials: D
authors.lastname: Yu
authors.forename: Ekambar R
authors.initials: ER
authors.lastname: Kandimalla
authors.forename: Sudhir
authors.initials: S
authors.lastname: Agrawal
mesh_terms: D000276:Adjuvants, Immunologic / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology*; D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D018925:Chemokines / Q000096:biosynthesis; D016207:Cytokines / Q000096:biosynthesis; D006801:Humans; D008024:Ligands; D051379:Mice; D009690:Nucleic Acid Conformation; D009843:Oligoribonucleotides / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology*; D012260:Ribonucleases / Q000737:chemistry; D051199:Toll-Like Receptor 7 / Q000819:agonists*; D051200:Toll-Like Receptor 8 / Q000819:agonists*
chemical_list: D000276:Adjuvants, Immunologic; D018925:Chemokines; D016207:Cytokines; D008024:Ligands; D009843:Oligoribonucleotides; D051199:Toll-Like Receptor 7; D051200:Toll-Like Receptor 8; D012260:Ribonucleases