Electricity generation using chocolate industry wastewater and its treatment in activated sludge based microbial fuel cell and analysis of developed microbial community in the anode chamber.
Feasibility of using chocolate industry wastewater as a substrate for electricity generation using activated sludge as a source of microorganisms was investigated in two-chambered microbial fuel cell. The maximum current generated with membrane and salt bridge MFCs was 3.02 and 2.3 A/m(2), respectively, at 100 ohms external resistance, whereas the maximum current generated in glucose powered MFC was 3.1 A/m(2). The use of chocolate industry wastewater in cathode chamber was promising with 4.1 mA current output. Significant reduction in COD, BOD, total solids and total dissolved solids of wastewater by 75%, 65%, 68%, 50%, respectively, indicated effective wastewater treatment in batch experiments. The 16S rDNA analysis of anode biofilm and suspended cells revealed predominance of beta-Proteobacteria clones with 50.6% followed by unclassified bacteria (9.9%), alpha-Proteobacteria (9.1%), other Proteobacteria (9%), Planctomycetes (5.8%), Firmicutes (4.9%), Nitrospora (3.3%), Spirochaetes (3.3%), Bacteroides (2.4%) and gamma-Proteobacteria (0.8%). Diverse bacterial groups represented as members of the anode chamber community.
Patil
Sunil A
SA
Department of Microbiology, University of Pune, Pune 411 007, Maharashtra, India.
Surakasi
Venkata Prasad
VP
Koul
Sandeep
S
Ijmulwar
Shrikant
S
Vivek
Amar
A
Shouche
Y S
YS
Kapadnis
B P
BP
D001419:Bacteria / Q000166:cytology* / Q000378:metabolism; D001674:Bioelectric Energy Sources / Q000382:microbiology*; D002099:Cacao / Q000737:chemistry*; D002999:Clone Cells; D003246:Conservation of Energy Resources; D003470:Culture Media; D004560:Electricity; D004566:Electrodes / Q000382:microbiology; D004573:Electrolytes; D005947:Glucose / Q000378:metabolism; D007220:Industrial Waste; D008567:Membranes, Artificial; D010802:Phylogeny; D020560:Proteobacteria / Q000166:cytology / Q000235:genetics; D011522:Protons; D012722:Sewage / Q000382:microbiology*; D012965:Sodium Chloride / Q000737:chemistry; D014865:Waste Disposal, Fluid; D018508:Water Purification
D003470:Culture Media; D004573:Electrolytes; D007220:Industrial Waste; D008567:Membranes, Artificial; D011522:Protons; D012722:Sewage; D012965:Sodium Chloride; D005947:Glucose