title: Short-term effects of synchrotron irradiation on vasculature and tissue in healthy mouse brain.
abstract: The purpose of this study is to measure the effects of a tomographic synchrotron irradiation on healthy mouse brain. The cerebral cortexes of healthy nude mice were irradiated with a monochromatic synchrotron beam of 79 keV at a dose of 15 Gy in accordance with a protocol of photoactivation of cisplatin previously tested in our laboratory. Forty-eight hours, one week and one month after irradiation, the blood brain barrier (BBB) permeability was measured in the irradiated area with intravital multiphoton microscopy using fluorescent dyes with molecular weights of 4 and 70 kDa. Vascular parameters and gliosis were also assessed using quantitative immunohistochemistry. No extravasation of the fluorescent dyes was observed in the irradiated area at any measurement time (48 h, 1 week, 1 month). It appears that the BBB remains impermeable to molecules with a molecular weight of 4 kDa and above. The vascular density and vascular surface were unaffected by irradiation and no gliosis was induced. These findings suggest that a 15 Gy/79 keV synchrotron irradiation does not induce important damage on brain vasculature and tissue on the short term following irradiation.
authors.affiliation: INSERM, U836, Grenoble Institut des Neurosciences, F-38042 Grenoble, France.
authors.forename: Clément
authors.initials: C
authors.lastname: Ricard
authors.forename: Manuel
authors.initials: M
authors.lastname: Fernández
authors.forename: Jérôme
authors.initials: J
authors.lastname: Gastaldo
authors.forename: Lucie
authors.initials: L
authors.lastname: Dupin
authors.forename: Laurette
authors.initials: L
authors.lastname: Somveille
authors.forename: Régine
authors.initials: R
authors.lastname: Farion
authors.forename: Herwig
authors.initials: H
authors.lastname: Requardt
authors.forename: Jean-Claude
authors.initials: JC
authors.lastname: Vial
authors.forename: Hélène
authors.initials: H
authors.lastname: Elleaume
authors.forename: Christoph
authors.initials: C
authors.lastname: Segebarth
authors.forename: Boudewijn
authors.initials: B
authors.lastname: van der Sanden
mesh_terms: D000818:Animals; D001485:Basement Membrane / Q000737:chemistry; D001812:Blood-Brain Barrier / Q000528:radiation effects*; D001921:Brain / Q000098:blood supply* / Q000473:pathology / Q000528:radiation effects*; D024141:Collagen Type IV / Q000032:analysis; D005260:Female; D005904:Glial Fibrillary Acidic Protein / Q000032:analysis; D005911:Gliosis / Q000473:pathology; D007150:Immunohistochemistry; D051379:Mice; D008819:Mice, Nude; D011879:Radiotherapy Dosage; D017356:Synchrotrons
chemical_list: D024141:Collagen Type IV; D005904:Glial Fibrillary Acidic Protein