[Comparative characteristics of immunological tests in differentiation of systemic and cutaneous forms of lupus erythematosus].
OBJECTIVE
To study immune disorders in cell immunity and cytokine profile in integument lupus erythematosus (ILE) and systemic lupus erythematosus (SLE).


METHODS
Lymphocyte phenotype, oxygen-dependent and non-oxygen-dependent functions of circulating neutrophils and monocytes, serum levels of circulating immune complexes (CIC), cytokines (IL-1beta, IL-4, IL-6, TNFalpha), antibodies to aDNA were studied in 177 patients with lupus erythematosus aged 20-60 years (143 had SLE and 34 ILE) and 33 healthy controls.


RESULTS
CIC levels, serum aDNA, IL-1beta and IL-4, oxygen-dependent neutrophil metabolism were higher both in SLE and ILE. Only SLE was characterized by high oxygen stress of monocytes, acid phosphatase and cation proteins in neutrophils and monocytes. Lymphocyte phenotype both in SLE and ILE was characterized by low count of CD3+ lymphocytes and high count of cells with markers of activation and apoptosis. SLE patients had lower count of HLA-DR+ cells and higher count of CD16+ and CD25+ lymphocytes.


CONCLUSIONS
Identification of metabolic functions of circulating phagocytes, serum cytokines and lymphocyte phenotype can be used for differentiation between SLE and ILE.
Romanova
N V
NV
Shilkina
N P
NP
D000328:Adult; D016207:Cytokines / Q000097:blood; D003937:Diagnosis, Differential; D005260:Female; D006801:Humans; D007111:Immunity, Cellular; D007159:Immunologic Tests; D008178:Lupus Erythematosus, Cutaneous / Q000097:blood / Q000175:diagnosis* / Q000276:immunology*; D008180:Lupus Erythematosus, Systemic / Q000097:blood / Q000175:diagnosis* / Q000276:immunology*; D008214:Lymphocytes / Q000276:immunology; D008297:Male; D008875:Middle Aged; D010586:Phagocytes / Q000276:immunology; D055815:Young Adult
D016207:Cytokines