Aerobic biodegradation characteristics and metabolic products of quinoline by a Pseudomonas strain.
A bacterial strain, BW003, which utilized quinoline as its sole C, N and energy source, was isolated and identified as Pseudomonas sp. BW003 degraded 192-911 mg/l quinoline within 3-8 h with removal rates ranging from 96% to 98%. The optimum conditions for the degradation were 30 degrees C and pH 8. In the process of biodegradation, at least 43% of quinoline was transformed into 2-hydroxyquinoline, then 0.69% of 2-hydroxyquinoline was transformed into 2,8-dihydroxyquinoline, and then, presumably, into 8-hydroxycoumarin. Meanwhile, at least 48% of the nitrogen in quinoline was directly transformed into ammonia-N. An extra carbon source enhanced the nitrogen transformation from ammonia-N. Further experiments showed that, besides cell synthesis, BW003 transformed less than 6% of ammonia-N into nitrate through heterotrophic nitrification. In addition, BW003 contained a large plasmid, which may be involved in quinoline metabolism. The study indicates that quinoline and its metabolic products can be eliminated from wastewater by controlling the C/N ratio using BW003 as the bioaugmentation inoculum.
Sun
Qinghua
Q
College of Environmental Sciences and Engineering, Peking University, Beijing 100871, People's Republic of China.
Bai
Yaohui
Y
Zhao
Cui
C
Xiao
Yana
Y
Wen
Donghui
D
Tang
Xiaoyan
X
D000332:Aerobiosis / Q000187:drug effects; D000641:Ammonia / Q000032:analysis; D001673:Biodegradation, Environmental / Q000187:drug effects; D003470:Culture Media; D008401:Gas Chromatography-Mass Spectrometry; D005798:Genes, Bacterial; D006863:Hydrogen-Ion Concentration / Q000187:drug effects; D007287:Inorganic Chemicals / Q000032:analysis; D009584:Nitrogen / Q000032:analysis; D009930:Organic Chemicals / Q000032:analysis; D010957:Plasmids / Q000302:isolation & purification; D016133:Polymerase Chain Reaction; D011549:Pseudomonas / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D011804:Quinolines / Q000378:metabolism* / Q000494:pharmacology; D013696:Temperature
D003470:Culture Media; D007287:Inorganic Chemicals; D009930:Organic Chemicals; D011804:Quinolines; D000641:Ammonia; C037219:quinoline; D009584:Nitrogen