title: Adiponectin deficiency modulates adhesion molecules expression and cytokine production but does not affect disease severity in the transfer model of colitis.
abstract: We investigated the effect of adiponectin (APN) deficiency in the CD4(+)CD45RB(high) transfer model of colitis. Recombination activating gene (Rag)-1 knockout (KO) and Rag-1 APN KO mice receiving CD4(+)CD45RB(high) cells developed colitis of comparable severity. Colonic mRNA expression of IL-6 and IL-17 was lower in Rag-1 APN KO mice compared to Rag-1 KO mice. Rag-1 APN KO and Rag-1 KO mice released comparable amounts of IL-6 from colon cultures, whereas release of IL-17 was higher in Rag-1 APN KO compared to Rag-1 KO mice. Expression of TNFalpha mRNA was comparable in Rag-1 KO and Rag-1 APN KO mice, but protein release was lower in Rag-1 APN KO mice compared to Rag-1 KO mice. Levels of IFNgamma and IL-10 at mRNA and protein were comparable in Rag-1 KO and Rag-1 APN KO mice. Higher mRNA expression of VCAM-1 was observed in the colon of healthy APN KO compared to WT mice, while induction of colitis resulted in a comparable increase in VCAM-1 expression in Rag-1 KO and Rag-1 APN KO mice. In conclusion, although APN regulates expression of cytokines and adhesion molecules in the colon, this does not result in alteration of overall colitis severity in the CD4(+)CD45RB(high) transfer model.
authors.affiliation: Department of Kinesiology and Nutrition, University of Illinois at Chicago, Chicago, IL 60612, USA.
authors.forename: Melissa E
authors.initials: ME
authors.lastname: Gove
authors.forename: Maria
authors.initials: M
authors.lastname: Pini
authors.forename: Raja
authors.initials: R
authors.lastname: Fayad
authors.forename: Robert J
authors.initials: RJ
authors.lastname: Cabay
authors.forename: Giamila
authors.initials: G
authors.lastname: Fantuzzi
mesh_terms: D052242:Adiponectin / Q000172:deficiency / Q000235:genetics / Q000378:metabolism; D000818:Animals; D015815:Cell Adhesion Molecules / Q000378:metabolism*; D003092:Colitis / Q000276:immunology* / Q000473:pathology; D003106:Colon / Q000276:immunology / Q000473:pathology; D016207:Cytokines / Q000096:biosynthesis*; D004195:Disease Models, Animal; D018398:Homeodomain Proteins / Q000235:genetics; D020738:Leptin / Q000378:metabolism; D017493:Leukocyte Common Antigens / Q000032:analysis; D007962:Leukocytes / Q000145:classification; D017710:Lymphocyte Transfusion; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout
chemical_list: D052242:Adiponectin; C499919:Adipoq protein, mouse; D015815:Cell Adhesion Molecules; D016207:Cytokines; D018398:Homeodomain Proteins; D020738:Leptin; C064658:RAG-1 protein; D017493:Leukocyte Common Antigens