title: [Human foreskin acellular matrix graft: a good scaffold for urethral tissue engineering].
abstract: OBJECTIVE
  To provide evidence of using the human foreskin acellular matrix graft for urethral tissue engineering. METH-ODS: The human foreskin acellular matrix graft was prepared, its safety and biocompatibility as urethral material were determined by histological observation, cytotoxicity test using primary epithelial cells and experiment in vivo.
  
  
  RESULTS
  Intact cells were absent from the foreskin acellular matrix graft. The cytotoxicity test showed that the relative growth rate of the cells was between 75% and 99%, and the cytotoxicity of the foreskin acellular matrix graft was grade 1, consistent with the national standard. With the lengthening of time, the foreskin acellular matrix graft became perfectly compatible with the urothelial cells and the urethral multi-layer structure was restored to normal gradually.
  
  
  CONCLUSIONS
  The human foreskin acellular matrix graft, with its low antigenicity and good biocompatibility, could be a good scaffold for urethral tissue engineering.
authors.affiliation: Department of Urology, The Ninth People's Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200011, China.
authors.forename: Hua
authors.initials: H
authors.lastname: Jiang
authors.forename: Li-Min
authors.initials: LM
authors.lastname: Ma
authors.forename: Juan
authors.initials: J
authors.lastname: Zhou
authors.forename: Mu-Jun
authors.initials: MJ
authors.lastname: Lu
authors.forename: Zhi-Kang
authors.initials: ZK
authors.lastname: Cai
authors.forename: Hao
authors.initials: H
authors.lastname: Wang
authors.forename: Jun
authors.initials: J
authors.lastname: Da
authors.forename: Qi
authors.initials: Q
authors.lastname: Chen
authors.forename: Zhong
authors.initials: Z
authors.lastname: Wang
mesh_terms: D002478:Cells, Cultured; D052816:Foreskin / Q000166:cytology / Q000637:transplantation*; D006801:Humans; D008297:Male; D019651:Plastic Surgery Procedures / Q000379:methods*; D046509:Tissue Culture Techniques; D023822:Tissue Engineering / Q000379:methods*; D014521:Urethra / Q000601:surgery*