title: Time-resolved tracking of a minimum gene expression system reconstituted in giant liposomes.
abstract: Individual expression: We describe a method that allows the observation of real-time gene expression in a large number of individual giant liposomes encapsulating identical genetic material. We followed the gene expression profiles from DNA and mRNA templates coding for different proteins. Although the average profiles of individual liposomes were similar to those measured in bulk solution, strong variability between individual liposomes was observed at both transcription and translation.
authors.affiliation: Department of Gene Mechanisms, Graduate School of Biostudies, Kyoto University, Kyoto, Japan. h-saito@kuchem.kyoto-u.ac.jp
authors.forename: Hirohide
authors.initials: H
authors.lastname: Saito
authors.forename: Yusho
authors.initials: Y
authors.lastname: Kato
authors.forename: Maël
authors.initials: M
authors.lastname: Le Berre
authors.forename: Ayako
authors.initials: A
authors.lastname: Yamada
authors.forename: Tan
authors.initials: T
authors.lastname: Inoue
authors.forename: Kenichi
authors.initials: K
authors.lastname: Yosikawa
authors.forename: Damien
authors.initials: D
authors.lastname: Baigl
mesh_terms: D004247:DNA / Q000378:metabolism; D015870:Gene Expression; D020869:Gene Expression Profiling; D049452:Green Fluorescent Proteins / Q000235:genetics; D008081:Liposomes / Q000737:chemistry* / Q000494:pharmacology; D012333:RNA, Messenger / Q000378:metabolism; D013997:Time Factors
chemical_list: D008081:Liposomes; D012333:RNA, Messenger; D049452:Green Fluorescent Proteins; D004247:DNA