Transient gene suppression in a red alga, Cyanidioschyzon merolae 10D.
Antisense suppression is a powerful tool to analyze gene function. In this study, we show that antisense RNA suppressed the expression of a target gene in the unicellular red alga, Cyanidioschyzon merolae. In this study, the antisense strand of the catalase gene was cloned and inserted into an expression vector upstream of the GFP gene. This plasmid was introduced into C. merolae cells using a polyethylene glycol-mediated transformation protocol. Using the expression of GFP as a marker of transformed cells, the expression of catalase was examined by immunocytochemistry. Decreased expression of catalase was observed in cells that were transformed with the antisense strand of the catalase gene. These results indicate the utility of this antisense suppression system.
Ohnuma
Mio
M
Research Information Center for Extremophile, Rikkyo University, 3-34-1 Nishiikebukuro, Toshima-ku, Tokyo, 171-8501, Japan. mioohnuma@rikkyo.ac.jp
Misumi
Osami
O
Fujiwara
Takayuki
T
Watanabe
Satoru
S
Tanaka
Kan
K
Kuroiwa
Tsuneyoshi
T
D002374:Catalase / Q000235:genetics / Q000378:metabolism; D015971:Gene Expression Regulation, Enzymologic; D007150:Immunohistochemistry; D016372:RNA, Antisense / Q000235:genetics / Q000502:physiology; D000461:Rhodophyta / Q000201:enzymology / Q000235:genetics*
D016372:RNA, Antisense; D002374:Catalase