Monocular deprivation provokes alteration of the neuronal cytoskeleton in developing cat lateral geniculate nucleus.
Monocular deprivation early in development produces considerable change in the organization of connections within the central mammalian visual system. In the dorsal lateral geniculate nucleus, the soma, dendrites, and axon terminal fields of deprived cells become considerably smaller than nondeprived counterparts. We have examined the possibility that subcellular events enabling structural modification of deprived neurons include modification of proteins comprising the cytoskeleton. We examined the integrity of the cytoskeleton by measuring the response of a subset of its proteins to varying durations of monocular deprivation. Loss of all three neurofilament subunits (light, medium, and heavy) within deprived layers was observed to parallel changes in neuron gross structure. Monocular deprivation initiated beyond early life produced neither a change in structure nor a loss of neurofilament labeling.
Duffy
Kevin R
KR
Department of Psychology/Neuroscience, Dalhousie University, Life Sciences Centre, Nova Scotia, Canada. kevin.duffy@dal.ca
Slusar
Joanna E
JE
D000818:Animals; D000831:Animals, Newborn; D002415:Cats; D003599:Cytoskeleton / Q000648:ultrastructure*; D003712:Dendrites / Q000648:ultrastructure; D005829:Geniculate Bodies / Q000166:cytology* / Q000254:growth & development* / Q000378:metabolism / Q000502:physiology; D016900:Neurofilament Proteins / Q000378:metabolism; D009473:Neuronal Plasticity / Q000502:physiology*; D009474:Neurons / Q000378:metabolism / Q000648:ultrastructure*; D017729:Presynaptic Terminals / Q000648:ultrastructure; D012683:Sensory Deprivation / Q000502:physiology*; D015349:Vision, Monocular / Q000502:physiology*
D016900:Neurofilament Proteins