Rapid synthesis, screening, and identification of xanthone- and xanthene-based fluorophores using click chemistry.
A panel of new fluorophores with emission wavelengths from blue to yellow regions using the Cu(I)-catalyzed 1,3-dipolar cycloaddition reaction of alkyne-functionalized xanthones and xanthenes with various azides have been synthesized. Screening of the "click" products led to the identification of "hit" fluorophores which showed a fluorescence increase upon triazole formation. These novel "click" fluorophores could potentially be used for bioconjugation and bioimaging purposes.
Li
Junqi
J
Department of Chemistry, NUS MedChem Program of the Life Sciences Institute, National University of Singapore, Singapore 117543.
Hu
Mingyu
M
Yao
Shao Q
SQ
D000480:Alkynes / Q000737:chemistry; D001386:Azides / Q000737:chemistry; D002384:Catalysis; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000138:chemical synthesis* / Q000737:chemistry; D015394:Molecular Structure; D014966:Xanthenes / Q000138:chemical synthesis* / Q000737:chemistry; D044004:Xanthones / Q000138:chemical synthesis* / Q000737:chemistry
D000480:Alkynes; D001386:Azides; D005456:Fluorescent Dyes; D014966:Xanthenes; D044004:Xanthones