title: Reduced NGF secretion by HT-29 human colon cancer cells treated with a GRPR antagonist.
abstract: The gastrin-releasing peptide receptor (GRPR) is a therapeutic target in colon cancer. Here we show that the GRPR antagonist RC-3095 (10(-3), 10(-6), or 1 microM) decreases nerve growth factor (NGF) secretion measured by enzyme-linked immunosorbent assay (ELISA) in HT-29 human colon carcinoma cells. The results suggest that decreased secretion of neurotrophins might be a novel mechanism by which GRPR antagonists exert their antiproliferative effects in cancer cells.
authors.affiliation: Cancer Research Laboratory, Academic Hospital Research Center, Federal University of Rio Grande do Sul, Porto Alegre, RS, Brazil.
authors.forename: Caroline Brunetto
authors.initials: CB
authors.lastname: de Farias
authors.forename: Laura
authors.initials: L
authors.lastname: Stertz
authors.forename: Rodrigo Cruz
authors.initials: RC
authors.lastname: Lima
authors.forename: Flávio
authors.initials: F
authors.lastname: Kapczinski
authors.forename: Gilberto
authors.initials: G
authors.lastname: Schwartsmann
authors.forename: Rafael
authors.initials: R
authors.lastname: Roesler
mesh_terms: D000704:Analysis of Variance; D001839:Bombesin / Q000031:analogs & derivatives* / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004797:Enzyme-Linked Immunosorbent Assay; D019073:HT29 Cells; D006801:Humans; D020932:Nerve Growth Factor / Q000378:metabolism*; D010446:Peptide Fragments / Q000494:pharmacology*; D018004:Receptors, Bombesin / Q000037:antagonists & inhibitors*
chemical_list: C502240:NGF protein, human; D010446:Peptide Fragments; D018004:Receptors, Bombesin; C071363:bombesin (6-14), Tpi(6)-Leu(13)-psi(CH2NH)-Leu(14)-; D020932:Nerve Growth Factor; D001839:Bombesin