Occurrence and genotypes of Giardia isolated from lambs in Spain.
Three hundred and eighty six faecal specimens were randomly collected from 1- to 3-month-old lambs from 16 farms in Spain to investigate the presence of different genotypes of Giardia duodenalis. Individual specimens were examined by IFA (Immunofluorescence assay) and beta-giardin PCR polymerase chain reaction. Cysts of G. duodenalis were shed by lambs in every flock analyzed, showing a prevalence by farms of 100%. The average prevalence of G. duodenalis for the 386 specimens was 42%, ranging from 8.3 to 80% depending on the farm. beta-giardin PCR positive samples were sequenced to determine the genotypes present at each farm and seven new subtypes of beta-giardin Assemblage E are reported in this study. In each farm, one to six different beta-giardin subtypes were found, showing the high variability of the target. Also, one flock had the zoonotic Assemblage A. This is the first report of Giardia subgenotype A-1 in sheep in Spain.
Gómez-Muñoz
María Teresa
MT
Departamento de Producción Animal, Sanidad Animal y Ciencia y Tecnología de los Alimentos, Facultad de Veterinaria, Universidad CEU Cardenal Herrera, Avda. Seminario S/N 46113 Moncada, Valencia, Spain. mtgomez@uch.ceu.es
Navarro
Cristina
C
Garijo-Toledo
María Magdalena
MM
Dea-Ayuela
María Auxiliadora
MA
Fernández-Barredo
Salceda
S
Pérez-Gracia
María Teresa
MT
Domínguez-Márquez
María Victoria
MV
Borrás
Rafael
R
D000818:Animals; D003598:Cytoskeletal Proteins / Q000235:genetics; D005243:Feces / Q000469:parasitology; D005455:Fluorescent Antibody Technique / Q000379:methods; D005838:Genotype; D005872:Giardia / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D005873:Giardiasis / Q000453:epidemiology / Q000469:parasitology / Q000662:veterinary*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000662:veterinary; D015995:Prevalence; D015800:Protozoan Proteins / Q000235:genetics; D017422:Sequence Analysis, DNA; D012756:Sheep / Q000469:parasitology*; D012757:Sheep Diseases / Q000453:epidemiology* / Q000469:parasitology; D013030:Spain / Q000453:epidemiology; D015047:Zoonoses / Q000453:epidemiology / Q000469:parasitology
D003598:Cytoskeletal Proteins; D015800:Protozoan Proteins; C038207:giardin protein, Giardia lamblia