title: [Extraction of lobetyolin from codonopsis with supercritical CO2].
abstract: OBJECTIVE
  To develop a green and rapid method for extraction of lobetyolin from C. pilosula.
  
  
  METHODS
  Extraction of lobetyolin from C. pilosula with supercritical carbon dioxide in the presence of ethanol was studied. The effects of pressure, temperature, volume of cosolvent and extraction time on efficiency and their interactive relationships were discussed, based on central composite design and response surface methodology (RSM).
  
  
  RESULTS
  The key effect factor was volume of cosolvent. The extraction yield of lobetyolin was 0.078 6 mg x g(-1) when C. pilosula (40-60 mesh) was extracted at 30 MPa, 60 degrees C and 2 L x min(-1) (as CO2 in normal pressure and temperature) for 100 minutes with supercritical CO2 and 1 mL x min(-1) ethanol as dynamic cosolvent.
  
  
  CONCLUSIONS
  This result is better than that obtained from traditional method. Therefore, the optimized process is valuable for extraction of lobetyolin from C. pilosula.
authors.affiliation: Key Laboratory for Green Chemical Technology of State Education Ministry, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China.
authors.forename: Tongju
authors.initials: T
authors.lastname: Liu
authors.forename: Shufen
authors.initials: S
authors.lastname: Li
authors.forename: Jiang
authors.initials: J
authors.lastname: Min
authors.forename: Xiaomei
authors.initials: X
authors.lastname: Bao
mesh_terms: D002245:Carbon Dioxide / Q000737:chemistry*; D005591:Chemical Fractionation / Q000379:methods*; D029745:Codonopsis / Q000737:chemistry*; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D000431:Ethanol / Q000737:chemistry; D053279:Polyynes / Q000737:chemistry*
chemical_list: D004365:Drugs, Chinese Herbal; C521561:lobetyolin; D002245:Carbon Dioxide; D053279:Polyynes; D000431:Ethanol