title: KLOTHO gene polymorphisms G-395A and C1818T are associated with lipid and glucose metabolism, bone mineral density and systolic blood pressure in Japanese healthy subjects.
abstract: BACKGROUND
  We investigated the association of KLOTHO gene single nucleotide polymorphisms (SNPs) with various laboratory data in 476 Japanese healthy subjects.
  
  
  METHODS
  The genotyping of G-395A in the promoter region and C1818T in exon 4 was performed using PCR with confronting 2-pair primers assay.
  
  
  RESULTS
  Multivariate analysis adjusted for age demonstrated that in men, body-fat ratio was high, and HDL cholesterol level was low in A allele carriers of G-395A compared with GG. In women, glucose was high in A allele carriers of G-395A compared with GG, and also in T allele carriers of C1818T compared with CC. When divided into 2 groups according to age, in men <60 y, body mass index, body-fat ratio and waist circumference were high in A carriers of G-395A compared with GG. In women <60 y, bone mineral density was high in A allele carriers of G-395A compared with GG, and systolic blood pressure and glucose were high in T carriers of C1818T compared with CC.
  
  
  CONCLUSIONS
  KLOTHO gene SNPs G-395A and C1818T are associated with lipid metabolism in men, and glucose metabolism, bone mineral density and systolic blood pressure in women.
authors.affiliation: Clinical Preventive Medicine, Nagoya University Graduate School of Medicine, 65 Tsurumai-cho, Nagoya 466-8550, Japan.
authors.forename: Yasuhiko
authors.initials: Y
authors.lastname: Shimoyama
authors.forename: Kazuko
authors.initials: K
authors.lastname: Nishio
authors.forename: Nobuyuki
authors.initials: N
authors.lastname: Hamajima
authors.forename: Toshimitsu
authors.initials: T
authors.lastname: Niwa
mesh_terms: D000375:Aging / Q000235:genetics / Q000378:metabolism / Q000502:physiology; D044466:Asian People / Q000235:genetics*; D001794:Blood Pressure / Q000235:genetics*; D015519:Bone Density / Q000235:genetics*; D005091:Exons / Q000235:genetics; D005260:Female; D005838:Genotype; D005947:Glucose / Q000378:metabolism*; D005966:Glucuronidase / Q000235:genetics*; D006801:Humans; D007564:Japan; D000090265:Klotho Proteins; D050356:Lipid Metabolism / Q000235:genetics*; D008297:Male; D008593:Menopause / Q000235:genetics / Q000502:physiology; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D011401:Promoter Regions, Genetic / Q000235:genetics
chemical_list: D005966:Glucuronidase; D000090265:Klotho Proteins; D005947:Glucose