Histological and ultrastructural observation reveals significant cellular differences between Agrobacterium transformed embryogenic and non-embryogenic calli of cotton.
Over the past few decades genetic engineering has been applied to improve cotton breeding. Agrobacterium medicated transformation is nowadays widely used as an efficient approach to introduce exogenous genes into cotton for genetically modified organisms. However, it still needs to be improved for better transformation efficiency and higher embryogenic callus induction ratios. To research further the difference of mechanisms for morphogenesis between embryogenic callus and non-embryogenic callus, we carried out a systematical study on the histological and cellular ultrastructure of Agrobacterium transformed calli. Results showed that the embryogenic callus developed nodule-like structures, which were formed by small, tightly packed, hemispherical cells. The surface of some embryogenic callus was covered with a fibrilar-like structure named extracellular matrix. The cells of embryogenic calli had similar morphological characteristics. Organelles of embryogenic callus cells were located near the nucleus, and chloroplasts degraded to proplastid-like structures with some starch grains. In contrast, the non-embryogenic calli were covered by oval or sphere cells or small clusters of cells. It was observed that cells had vacuolation of cytoplasm and plastids with a well organized endomembrane system. This study aims to understand the mechanisms of embryogenic callus morphogenesis and to improve the efficiency of cotton transformation in future.
Shang
Hai-Hong
HH
Key Laboratory of Cotton Genetic Improvement, Ministry of Agriculture, Chinese Cotton Research Institute, Chinese Agricultural Academy of Sciences, Anyang 455004, China.
Liu
Chuan-Liang
CL
Zhang
Chao-Jun
CJ
Li
Feng-Lian
FL
Hong
Wei-Dong
WD
Li
Fu-Guang
FG
D018506:Gene Expression Regulation, Plant; D003368:Gossypium / Q000196:embryology / Q000235:genetics / Q000382:microbiology* / Q000648:ultrastructure*; D046529:Microscopy, Electron, Transmission; D008856:Microscopy, Fluorescence; D030821:Plants, Genetically Modified / Q000196:embryology / Q000235:genetics / Q000382:microbiology* / Q000648:ultrastructure*; D012231:Rhizobium / Q000235:genetics / Q000502:physiology*; D014170:Transformation, Genetic / Q000235:genetics / Q000502:physiology