Isolation and partial characterization of a novel lipid transfer protein (LTP) and antifungal activity of peptides from chilli pepper seeds.
The aims of this study were to isolate and characterize peptides present in chilli pepper seeds and evaluate their antifungal activities. An isolated peptide closer to 9 kDa showed high sequence homology to the antimicrobial peptide lipid transfer protein. The peptide fraction containing the LTP inhibited the growth of the fungi, Fusarium oxysporum, Colletotrium lindemunthianum, the yeasts, Saccharomyces cerevisiae, Pichia membranifaciens, Candida tropicalis, Candida albicans, inhibited glucose-stimulated acidification of the medium by yeast cells of S. cerevisiae and caused several morphological changes in P. membranifaciens.
Cruz
Luana P
LP
Universidade Estadual do Norte Fluminense, Centro de Biociôncias e Biotecnologia, Campos dos Goytacazes, 28015-602, RJ, Brazil.
Ribeiro
Suzanna F F
SF
Carvalho
André O
AO
Vasconcelos
Ilka M
IM
Rodrigues
Rosana
R
Da Cunha
Maura
M
Gomes
Valdirene M
VM
D000595:Amino Acid Sequence; D000935:Antifungal Agents / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D052179:Antigens, Plant / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D023181:Antimicrobial Cationic Peptides / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D002212:Capsicum / Q000737:chemistry*; D002352:Carrier Proteins / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D002852:Chromatography, Ion Exchange; D004591:Electrophoresis, Polyacrylamide Gel; D005658:Fungi / Q000187:drug effects / Q000254:growth & development; D008856:Microscopy, Fluorescence; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D012639:Seeds / Q000737:chemistry
D000935:Antifungal Agents; D052179:Antigens, Plant; D023181:Antimicrobial Cationic Peptides; D002352:Carrier Proteins; D010940:Plant Proteins; C511920:lipid transfer proteins, plant