Expression of NAD+-dependent formate dehydrogenase in Enterobacter aerogenes and its involvement in anaerobic metabolism and H2 production.
An expression system for NAD(+)-dependent formate dehydrogenase gene (fdh1), from Candida boidinii, was constructed and cloned into Enterobacter aerogenes IAM1183. With the fdh1 expression, the total H(2) yield was attributed to a decrease in activity of the lactate pathway and an increase of the formate pathway flux due to the NADH regeneration. Analysis of the redox state balance and ethanol-to-acetate ratio in the fdhl-expressed strain showed that increased reducing power arose from the reconstruction of NADH regeneration pathway from formate thereby contributing to the improved H(2) production.
Lu
Yuan
Y
Institute of Biochemical Engineering, Department of Chemical Engineering, Tsinghua University, Beijing, 100084, China.
Zhao
Hongxin
H
Zhang
Chong
C
Lai
Qiheng
Q
Wu
Xi
X
Xing
Xin-Hui
XH
D019342:Acetic Acid / Q000032:analysis; D000693:Anaerobiosis; D002175:Candida / Q000201:enzymology* / Q000235:genetics; D003600:Cytosol / Q000737:chemistry; D021902:Enterobacter aerogenes / Q000235:genetics / Q000502:physiology*; D000431:Ethanol / Q000032:analysis; D005560:Formate Dehydrogenases / Q000096:biosynthesis* / Q000235:genetics; D015870:Gene Expression; D006859:Hydrogen / Q000378:metabolism*; D009243:NAD / Q000378:metabolism; D010084:Oxidation-Reduction; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics
D011994:Recombinant Proteins; D009243:NAD; D000431:Ethanol; D006859:Hydrogen; D005560:Formate Dehydrogenases; D019342:Acetic Acid