Synthesis of a novel biodegradable poly(ester amine) (PEAs) copolymer based on low-molecular-weight polyethyleneimine for gene delivery.
In this paper, a novel biodegradable poly(ester amine) (PEA) copolymer was successfully prepared from low-molecular-weight polyethyleneimine (PEI, Mn=1800) and poly(epsilon-caprolactone)-Pluronic-poly(epsilon-caprolactone) (PCFC) copolymers. According to the results of agarose gel electrophoresis, particle sizes and zeta potential measurement and transfection efficiency, these PEA copolymers showed great ability to condense plasmid DNA effectively into nano-complexes with small particle size (< or =200 nm) and moderate zeta potential (> or =12 mV) at proper polymeric carrier/DNA weight ratio. Compared with low-molecular-PEI (Mn=1800), the obtained PEAs exhibited higher transfection efficiency as well as lower cytotoxicity. These results indicated that such PEAs might have great potential application in gene delivery system.
Guo
Qingfa
Q
State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, West China Medical School, Sichuan University, Chengdu, 610041, China.
Shi
Shuai
S
Wang
Xiuhong
X
Kan
Bing
B
Gu
Yingchun
Y
Shi
Xingyu
X
Luo
Feng
F
Zhao
Xia
X
Wei
Yuquan
Y
Qian
Zhiyong
Z
D002460:Cell Line; D004247:DNA / Q000008:administration & dosage; D004587:Electrophoresis, Agar Gel; D018014:Gene Transfer Techniques; D006801:Humans; D010316:Particle Size; D010957:Plasmids / Q000008:administration & dosage; D020442:Poloxamer / Q000737:chemistry; D011073:Polyamines / Q000138:chemical synthesis*; D011091:Polyesters / Q000138:chemical synthesis* / Q000737:chemistry; D011094:Polyethyleneimine / Q000737:chemistry; D014162:Transfection
D011073:Polyamines; D011091:Polyesters; D020442:Poloxamer; C016240:polycaprolactone; D011094:Polyethyleneimine; D004247:DNA