Lactic acid bacteria affect serum cholesterol levels, harmful fecal enzyme activity, and fecal water content.
BACKGROUND
Lactic acid bacteria (LAB) are beneficial probiotic organisms that contribute to improved nutrition, microbial balance, and immuno-enhancement of the intestinal tract, as well as lower cholesterol. Although present in many foods, most trials have been in spreads or dairy products. Here we tested whether Bifidobacteria isolates could lower cholesterol, inhibit harmful enzyme activities, and control fecal water content.


METHODS
In vitro culture experiments were performed to evaluate the ability of Bifidobacterium spp. isolated from healthy Koreans (20 approximately 30 years old) to reduce cholesterol-levels in MRS broth containing polyoxyethanylcholesterol sebacate. Animal experiments were performed to investigate the effects on lowering cholesterol, inhibiting harmful enzyme activities, and controlling fecal water content. For animal studies, 0.2 ml of the selected strain cultures (108 approximately 109 CFU/ml) were orally administered to SD rats (fed a high-cholesterol diet) every day for 2 weeks.


RESULTS
B. longum SPM1207 reduced serum total cholesterol and LDL levels significantly (p < 0.05), and slightly increased serum HDL. B. longum SPM1207 also increased fecal LAB levels and fecal water content, and reduced body weight and harmful intestinal enzyme activities.


CONCLUSIONS
Daily consumption of B. longum SPM1207 can help in managing mild to moderate hypercholesterolemia, with potential to improve human health by helping to prevent colon cancer and constipation.
Lee
Do Kyung
DK
Department of Pharmacy, Sahmyook University, Seoul 139-742, Republic of Korea. 015790@hanmail.net
Jang
Seok
S
Baek
Eun Hye
EH
Kim
Mi Jin
MJ
Lee
Kyung Soon
KS
Shin
Hea Soon
HS
Chung
Myung Jun
MJ
Kim
Jin Eung
JE
Lee
Kang Oh
KO
Ha
Nam Joo
NJ
D000328:Adult; D000818:Animals; D001483:Base Sequence; D001644:Bifidobacterium / Q000187:drug effects / Q000302:isolation & purification / Q000502:physiology*; D001835:Body Weight / Q000187:drug effects; D002784:Cholesterol / Q000097:blood*; D015169:Colony Count, Microbial; D004041:Dietary Fats / Q000494:pharmacology; D005243:Feces / Q000737:chemistry / Q000201:enzymology* / Q000382:microbiology*; D006801:Humans; D007422:Intestines / Q000187:drug effects / Q000201:enzymology / Q000382:microbiology; D008297:Male; D008969:Molecular Sequence Data; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D014867:Water / Q000032:analysis*
D004041:Dietary Fats; D012336:RNA, Ribosomal, 16S; D014867:Water; D002784:Cholesterol