The protein import channel in the outer mitosomal membrane of Giardia intestinalis.
The identification of mitosomes in Giardia generated significant debate on the evolutionary origin of these organelles, whether they were highly reduced mitochondria or the product of a unique endosymbiotic event in an amitochondrial organism. As the protein import pathway is a defining characteristic of mitochondria, we sought to discover a TOM (translocase in the outer mitochondrial membrane) complex in Giardia. A Hidden Markov model search of the Giardia genome identified a Tom40 homologous sequence (GiTom40), where Tom40 is the protein translocation channel of the TOM complex. The GiTom40 protein is located in the membrane of mitosomes in a approximately 200-kDa TOM complex. As Tom40 was derived in the development of mitochondria to serve as the protein import channel in the outer membrane, its presence in Giardia evidences the mitochondrial ancestry of mitosomes.
Dagley
Michael J
MJ
Department of Biochemistry and Molecular Biology, University of Melbourne, Parkville, Australia.
Dolezal
Pavel
P
Likic
Vladimir A
VA
Smid
Ondrej
O
Purcell
Anthony W
AW
Buchanan
Susan K
SK
Tachezy
Jan
J
Lithgow
Trevor
T
D000595:Amino Acid Sequence; D000818:Animals; D019143:Evolution, Molecular; D016829:Giardia lamblia / Q000378:metabolism*; D047468:Immunoprecipitation; D008928:Mitochondria / Q000378:metabolism*; D051336:Mitochondrial Membranes / Q000378:metabolism*; D024101:Mitochondrial Proteins / Q000737:chemistry / Q000378:metabolism*; D008969:Molecular Sequence Data; D020836:Protein Structure, Quaternary; D021381:Protein Transport; D015800:Protozoan Proteins / Q000737:chemistry / Q000378:metabolism*; D016415:Sequence Alignment
D024101:Mitochondrial Proteins; D015800:Protozoan Proteins