The in vivo bone formation by mesenchymal stem cells in zein scaffolds.
In our previous study, a three-dimensional zein porous scaffold was prepared. This scaffold showed proper mechanical properties, good biocompatibility, and controllable biodegradation. In addition, it allowed blood vessels to form inside in vivo. In the current study, we prepared the complexes of zein scaffolds and rabbit MSCs, and investigated ectopic bone formation in nude mice. Furthermore, we implanted them into the radius defects of rabbits and assessed whether they could be helpful in the repair of critical-sized bone defects. The results showed that the complexes of zein scaffolds and rabbit MSCs could undergo ectopic bone formation in the thigh muscle pouches of nude mice. More importantly, the complexes could lead to the repair of critical-sized radius defects in rabbits accompanied with blood vessels' formation, which clearly demonstrates promise for the treatment of bone defects through tissue engineering.
Tu
Jinwen
J
The Key Laboratory of Stem Cell Biology, Institute of Health Sciences, Shanghai Jiao Tong University School of Medicine, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 225 South Chongqing Road, Shanghai 200025, PR China.
Wang
Huajie
H
Li
Huiwu
H
Dai
Kerong
K
Wang
Jinye
J
Zhang
Xiaoling
X
D000818:Animals; D020007:Implants, Experimental; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000187:drug effects* / Q000378:metabolism; D051379:Mice; D008819:Mice, Nude; D010012:Osteogenesis / Q000187:drug effects*; D011817:Rabbits; D011884:Radius / Q000000981:diagnostic imaging / Q000187:drug effects / Q000473:pathology; D054457:Tissue Scaffolds; D014945:Wound Healing / Q000187:drug effects; D055114:X-Ray Microtomography; D015028:Zein / Q000494:pharmacology*
D015028:Zein