title: Humoral and cell-mediated immune responses in DNA immunized mink challenged with wild-type canine distemper virus.
abstract: The aim of the study was to investigate the different phases of the immune response after DNA immunization with the hemagglutinin and nucleoprotein genes from canine distemper virus (CDV). Although attenuated live CDV vaccines have effectively reduced the incidence of disease, canine distemper is still a problem worldwide. The broad host range of CDV creates a constant viral reservoir among wildlife animals. Our results demonstrated early humoral and cell-mediated immune responses (IFN-gamma) in DNA vaccinated mink compared to mock-vaccinated mink after challenge with a Danish wild-type CDV. The DNA vaccine-induced immunity protected the natural host against disease development.
authors.affiliation: Department of Veterinary Disease Biology, Faculty of Life Sciences, University of Copenhagen, Stigbøjlen 7, Frederiksberg C, Denmark. lin@life.ku.dk
authors.forename: Line
authors.initials: L
authors.lastname: Nielsen
authors.forename: Mette
authors.initials: M
authors.lastname: Søgaard
authors.forename: Peter
authors.initials: P
authors.lastname: Karlskov-Mortensen
authors.forename: Trine Hammer
authors.initials: TH
authors.lastname: Jensen
authors.forename: Tove Dannemann
authors.initials: TD
authors.lastname: Jensen
authors.forename: Bent
authors.initials: B
authors.lastname: Aasted
authors.forename: Merete
authors.initials: M
authors.lastname: Blixenkrone-Møller
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D002531:Cerebellum / Q000821:virology; D054022:Cerebrum / Q000821:virology; D004216:Distemper / Q000276:immunology / Q000517:prevention & control*; D004217:Distemper Virus, Canine / Q000235:genetics / Q000276:immunology*; D006389:Hemagglutinins, Viral / Q000235:genetics / Q000276:immunology; D007371:Interferon-gamma / Q000378:metabolism; D008168:Lung / Q000821:virology; D008231:Lymphopenia / Q000517:prevention & control; D008907:Mink; D009698:Nucleoproteins / Q000235:genetics / Q000276:immunology; D013154:Spleen / Q000821:virology; D013601:T-Lymphocytes / Q000276:immunology; D001743:Urinary Bladder / Q000821:virology; D019444:Vaccines, DNA / Q000235:genetics / Q000276:immunology*; D015678:Viral Structural Proteins / Q000235:genetics / Q000276:immunology; D014766:Viremia / Q000517:prevention & control
chemical_list: D000914:Antibodies, Viral; D006389:Hemagglutinins, Viral; D009698:Nucleoproteins; D019444:Vaccines, DNA; D015678:Viral Structural Proteins; D007371:Interferon-gamma