Expression of IL-1beta, MMP-9 and TIMP-1 on the pressure side of gingiva under orthodontic loading.
OBJECTIVE
To test the hypothesis that orthodontic pressure does not induce gene transcription of IL-1beta, MMP-9, and TIMP-1 in pressure gingival soft tissue.


METHODS
A total of 14 male Wistar rats were used with three rats as no appliance controls and another three as the sham appliance group. On the 7th and the 14th day after orthodontic loading on the maxillary left molar, four rats were sacrificed, respectively. Maxillary right first molars served as the contralateral control side. A real-time RT-PCR for the excised gingiva was performed to measure the mRNA of IL-1beta, MMP-9, and TIMP-1.


RESULTS
Compared with the contralateral side, IL-1beta mRNA from the pressure side significantly increased on the 7th day, then decreased on the 14th day (P < .05). MMP-9 and TIMP-1 mRNA showed a significant constant increase on both the 7th and the 14th day (P < .05).


CONCLUSIONS
The hypothesis is rejected. Orthodontic loading led to increases in IL-1beta, MMP-9, and TIMP-1 mRNA in pressure side gingiva in rats.
Lee
Tae-Yeon
TY
Department of Orthodontics, College of Dentistry, Yonsei University, Seoul, South Korea.
Lee
Kee-Joon
KJ
Baik
Hyoung-Seon
HS
D000818:Animals; D003799:Dental Stress Analysis; D015870:Gene Expression; D005881:Gingiva / Q000378:metabolism*; D053583:Interleukin-1beta / Q000096:biosynthesis*; D008297:Male; D020780:Matrix Metalloproteinase 9 / Q000096:biosynthesis*; D009967:Orthodontic Appliances; D011312:Pressure; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017208:Rats, Wistar; D020133:Reverse Transcriptase Polymerase Chain Reaction; D019715:Tissue Inhibitor of Metalloproteinase-1 / Q000096:biosynthesis*; D014087:Tooth Movement Techniques
D053583:Interleukin-1beta; D012333:RNA, Messenger; D019715:Tissue Inhibitor of Metalloproteinase-1; D020780:Matrix Metalloproteinase 9