Dynamin participates in the maintenance of anterior polarity in the Caenorhabditis elegans embryo.
Cell polarity is crucial for the generation of cell diversity. Recent evidence suggests that the actin cytoskeleton plays a key role in establishment of embryonic polarity, yet the mechanisms that maintain polarity cues in particular membrane domains during development remain unclear. Dynamin, a large GTPase, functions in both endocytosis and actin dynamics. Here, the Caenorhabditis elegans dynamin ortholog, DYN-1, maintains anterior polarity cues. DYN-1-GFP foci are enriched in the anterior cortex in a manner dependent on the anterior polarity proteins, PAR-6 and PKC-3. Membrane internalization and actin comet formation are enriched in the anterior, and are dependent on DYN-1. PAR-6-labeled puncta are also internalized from cortical accumulations of DYN-1-GFP. Our results demonstrate a mechanism for the spatial and temporal regulation of endocytosis in the anterior of the embryo, contributing to the precise localization and maintenance of polarity factors within a dynamic plasma membrane.
Nakayama
Yuji
Y
Department of Genetics, University of Wisconsin-Madison, Madison, WI 53706, USA.
Shivas
Jessica M
JM
Poole
Daniel S
DS
Squirrell
Jayne M
JM
Kulkoski
Jennifer M
JM
Schleede
Justin B
JB
Skop
Ahna R
AR
D000199:Actins / Q000378:metabolism; D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D017173:Caenorhabditis elegans / Q000166:cytology* / Q000196:embryology*; D029742:Caenorhabditis elegans Proteins / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism; D016764:Cell Polarity; D034281:Dynamins / Q000378:metabolism*; D004625:Embryo, Nonmammalian / Q000166:cytology* / Q000201:enzymology; D004705:Endocytosis; D017353:Gene Deletion; D021381:Protein Transport; D008941:Spindle Apparatus / Q000378:metabolism
D000199:Actins; D015415:Biomarkers; D029742:Caenorhabditis elegans Proteins; C511792:Dyn-1 protein, C elegans; D034281:Dynamins