title: Ovarian toxicity of paclitaxel and effect on fertility in the rat.
abstract: OBJECTIVE
  Taxanes are regarded as key chemotherapy agents for breast cancer and gynecologic malignancies; therefore the ovarian toxicity of paclitaxel (PTX) is a matter of importance to younger women with malignancies. We examined the ovarian toxicity of PTX and its influence on fertility in rats.
  
  
  METHODS
  Female Wistar rats aged 6-8 weeks received five doses of PTX at 3-day intervals. Ovarian toxicity was assessed by counting follicles, corpora lutea and atretic follicles, as well as by detecting apoptosis and measuring serum levels of estradiol (E2) and progesterone (P4). Fertility was assessed by mating females immediately or 24 days after PTX treatment. The number of fetuses, implantations and resorption sites was counted in each group.
  
  
  RESULTS
  Exposure to PTX caused a decrease of antral follicles, but not primordial or pre-antral follicles. The number of corpora lutea showed a significant decrease, while follicular atresia was increased significantly. Apoptosis was only detected in antral follicles. Serum E2 levels were lower than in control rats, but not significantly, while P4 levels did not differ from those in control rats. Rats mated immediately after PTX treatment showed a significant decrease of fetuses and implantations, but these effects were not detected in rats mated at 24 days after treatment.
  
  
  CONCLUSIONS
  Our findings suggest that the ovarian toxicity of PTX is mild and transient. Use of PTX may help to maintain the fertility of younger women because the fertility of rats was not influenced at 24 days after exposure to this drug.
authors.affiliation: Department of Bioresource Sciences, Nihon University, Japan.
authors.forename: Wataru
authors.initials: W
authors.lastname: Tarumi
authors.forename: Nao
authors.initials: N
authors.lastname: Suzuki
authors.forename: Noriyuki
authors.initials: N
authors.lastname: Takahashi
authors.forename: Yoichi
authors.initials: Y
authors.lastname: Kobayashi
authors.forename: Kazushige
authors.initials: K
authors.lastname: Kiguchi
authors.forename: Kahei
authors.initials: K
authors.lastname: Sato
authors.forename: Bunpei
authors.initials: B
authors.lastname: Ishizuka
mesh_terms: D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000633:toxicity*; D017209:Apoptosis / Q000187:drug effects; D003338:Corpus Luteum / Q000187:drug effects; D004958:Estradiol / Q000097:blood; D005260:Female; D005298:Fertility / Q000187:drug effects*; D006080:Ovarian Follicle / Q000187:drug effects; D010053:Ovary / Q000187:drug effects*; D017239:Paclitaxel / Q000633:toxicity*; D011247:Pregnancy; D011374:Progesterone / Q000097:blood; D051381:Rats; D017208:Rats, Wistar
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D011374:Progesterone; D004958:Estradiol; D017239:Paclitaxel