title: Monitoring of glucose and glutamate using enzyme microstructures and scanning electrochemical microscopy.
abstract: Glucose oxidase and glutamate oxidase lines, with typical width of 100 microm, were patterned on gold surfaces using a micro-dispensing system, by shooting 100 pl droplets of the corresponding enzyme solutions. The probe of a scanning electrochemical microscope (SECM) was then carefully positioned in the close proximity of the enzyme microstructure and poised to +600 mV vs. Ag/AgCl, KCl 0.1 M. The H(2)O(2), generated by the enzyme lines at different concentrations of glucose and glutamate in the surrounding solution, was sequentially monitored. Reproducible calibration curves for glucose and glutamate were obtained in one single experiment, proving that the combination of enzyme microstructures with SECM can provide a new way of achieving multianalyte detection.
authors.affiliation: Department of Analytical Chemistry, Lund University, P.O. Box 124, SE-22100 Lund, Sweden. lamur@chem.ubbcluj.ro
authors.forename: Laura
authors.initials: L
authors.lastname: Mureşan
authors.forename: Mihaela
authors.initials: M
authors.lastname: Nistor
authors.forename: Szilveszter
authors.initials: S
authors.lastname: Gáspár
authors.forename: Ionel Cătălin
authors.initials: IC
authors.lastname: Popescu
authors.forename: Elisabeth
authors.initials: E
authors.lastname: Csöregi
mesh_terms: D001234:Aspergillus niger / Q000201:enzymology; D015374:Biosensing Techniques / Q000379:methods*; D002138:Calibration; D004563:Electrochemistry; D005947:Glucose / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D005949:Glucose Oxidase / Q000737:chemistry* / Q000378:metabolism; D018698:Glutamic Acid / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D008853:Microscopy; D015203:Reproducibility of Results
chemical_list: D018698:Glutamic Acid; D005949:Glucose Oxidase; D005947:Glucose