Identification of antigenic Brugia adult worm proteins by peptide mass fingerprinting.
With the recent completion of the Brugia malayi genome, proteomics offers a new resource for a deeper understanding of the biology of filarial parasites. We employed 2-dimensional (2D) gel electrophoresis followed by peptide mass fingerprinting on a matrix-assisted laser desorption/ionization time-of-flight (MALDI-ToF) mass spectrometer to identify Brugia adult worm proteins and then determined which proteins were recognized by the host humoral immune response. We identified 18 unique proteins, several of which were determined to be antigenic by immunoblot. The proteins identified here may contribute to future studies to analyze the transmission and pathogenesis of lymphatic filariasis.
Weinkopff
Tiffany
T
Department of Cell Biology, 724 Biological Sciences Building, University of Georgia, Athens, Georgia 30602, USA.
Atwood
James A
JA
Punkosdy
George A
GA
Moss
Delynn
D
Weatherly
D Brent
DB
Orlando
Ron
R
Lammie
Patrick
P
D000595:Amino Acid Sequence; D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood; D000947:Antigens, Helminth / Q000032:analysis* / Q000737:chemistry / Q000276:immunology; D017179:Brugia pahangi / Q000737:chemistry* / Q000276:immunology; D015180:Electrophoresis, Gel, Two-Dimensional; D004591:Electrophoresis, Polyacrylamide Gel; D004605:Elephantiasis, Filarial / Q000097:blood / Q000276:immunology / Q000469:parasitology; D005849:Gerbillinae; D015801:Helminth Proteins / Q000032:analysis* / Q000737:chemistry / Q000276:immunology; D006801:Humans; D007091:Image Processing, Computer-Assisted; D015151:Immunoblotting; D008969:Molecular Sequence Data; D040901:Proteomics; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D015801:Helminth Proteins