[Effects of different proportions of cryoprotectant to seminal plasma on the motility of post-thaw human sperm].
OBJECTIVE
To investigate the effects of different proportions of cryoprotectant to seminal plasma on the motility of post-thaw human sperm.


METHODS
Different proportions of cryoprotectant to seminal plasma (1:1 and 1:3) were used for freezing sperm, and the forward movement and total motility rates of the frozen-thawed sperm were compared.


RESULTS
The forward movement and total motility rates were (58.60 +/- 5.57)% and (66.17 +/- 5.24)% before cryopreservation. The 1:1 proportion achieved post-thaw forward movement and total motility rates of (40.53 +/- 8.97)% and (51.23 +/- 9. 30)%, while the 1:3 (44.7 +/- 8.67)% and (51.50 +/- 7.40)%, respectively. Significantly decreased sperm motility was observed after cryopreservation (P < 0.05). Statistically significant differences were found in the forward movement but not in the total motility of the frozen-thawed sperm between the two proportions.


CONCLUSIONS
Cryopreservation causes obvious damage to human sperm. Higher proportion of cryoprotectant to seminal plasma (1:3) can improve the forward movement of post-thaw sperm as compared with the lower one (1:1).
Hu
Jing-Mei
JM
Center for Reproductive Medicine, Shandong Provincial Hospital, Shandong University, Jinan, Shandong 250021, China. jingmei_hu8@hotmail.com
Xu
Cheng-Yan
CY
Li
Yuan
Y
Geng
Ling
L
Ma
Gang
G
Wang
Li
L
Chen
Zi-Jiang
ZJ
D000328:Adult; D003451:Cryoprotective Agents / Q000009:adverse effects / Q000494:pharmacology*; D005615:Freezing; D006801:Humans; D008297:Male; D012661:Semen / Q000187:drug effects*; D013081:Sperm Motility / Q000187:drug effects*
D003451:Cryoprotective Agents