Design and synthesis of membrane fusion inhibitors against the feline immunodeficiency virus.
Feline immunodeficiency virus (FIV) is a pathogenic virus that causes an AIDS-like syndrome in the domestic cats. For viral entry and infection, fusion between the virus and the cell membrane is the critical process and this process is mediated by an envelope glycoprotein gp40. We have identified fusion inhibitory peptides from the heptad repeat-2 (HR2) of gp40. Remodeling of the original sequences using alpha-helix-inducible motifs revealed the interactive residues of gp40. Comparative analysis of HR2 peptides derived from four FIV strains demonstrated that the interactive surface of the Shizuoka strain-derived HR2 peptides provides the highest affinity of all the FIV strains examined.
Oishi
Shinya
S
Graduate School of Pharmaceutical Sciences, Kyoto University, Sakyo-ku, Kyoto 606-8501, Japan. soishi@pharm.kyoto-u.ac.jp
Kodera
Yasuyo
Y
Nishikawa
Hiroki
H
Kamitani
Hirotaka
H
Watabe
Tsuyoshi
T
Ohno
Hiroaki
H
Tochikura
Tadafumi
T
Shimane
Kazuki
K
Kodama
Eiichi
E
Matsuoka
Masao
M
Mizukoshi
Fuminori
F
Tsujimoto
Hajime
H
Fujii
Nobutaka
N
D000595:Amino Acid Sequence; D000818:Animals; D002415:Cats; D016089:Immunodeficiency Virus, Feline / Q000187:drug effects*; D008561:Membrane Fusion / Q000187:drug effects*; D008969:Molecular Sequence Data; D010455:Peptides / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology*; D011485:Protein Binding; D017433:Protein Structure, Secondary; D020449:Repetitive Sequences, Amino Acid; D013329:Structure-Activity Relationship; D014759:Viral Envelope Proteins / Q000737:chemistry / Q000378:metabolism*
D010455:Peptides; D014759:Viral Envelope Proteins