[Evaluation of HbA1c using different methods in haemoglobin variant, Hb J-Bangkok].
A 31-year-old Japanese man with haemoglobin variant, Hb J-Bangkok [beta56 (D7) Gly-->Asp], was found by discrepant values between HbA1c and glycated-albumin. We measured HbA1c using three different methods, HPLC, enzyme assay and turbidimetric immunoassay. HbA1c value measured by HPLC was much lower than those by others. Furthermore, we estimated calculated glyco-haemoglobin value measured by high-resolution HPLC, revealing that HbA1c values measured by enzyme assay and turbidimetric immunoassay were comparable with calculated value. When measuring HbA1c value in haemoglobin variant, Hb J Bangkok, enzyme assay and turbidimetric immunoassay are useful methods.
Sawaragi
Wakana
W
Division of Laboratory and Transfusion Medicine, Hokkaido University Hospital, Sapporo 060-8648, Japan. xswakana@med.hokudai.ac.jp
Shibuya
Hitoshi
H
Yasuda
Keiko
K
Suzuki
Haruki
H
Shimizu
Chikara
C
Matsuno
Kazuhiko
K
D000328:Adult; D015415:Biomarkers / Q000032:analysis; D002851:Chromatography, High Pressure Liquid; D006442:Glycated Hemoglobin / Q000032:analysis*; D017127:Glycation End Products, Advanced; D006448:Hemoglobin J; D006453:Hemoglobinopathies / Q000097:blood* / Q000175:diagnosis*; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D007124:Immunoenzyme Techniques; D008297:Male; D009391:Nephelometry and Turbidimetry / Q000379:methods*; D012709:Serum Albumin / Q000032:analysis; D000093423:Glycated Serum Albumin
D015415:Biomarkers; D006442:Glycated Hemoglobin; D017127:Glycation End Products, Advanced; D006448:Hemoglobin J; D012709:Serum Albumin; D000093423:Glycated Serum Albumin; C517652:hemoglobin A1c protein, human; C029522:hemoglobin J Bangkok