title: Effects of ultraviolet radiation on human cutaneous nerve fibres.
abstract: Due to an increasing number of skin diseases as a result of exposure to ultraviolet (UV) radiation, it is necessary to evaluate the effectiveness of new skin care formulations with broad-spectrum sunscreens.
  
  
  OBJECTIVE
  This study aims to assess the status of nerve fibres in healthy human skin, to quantify effects of UV radiation on nerve endings, and to evaluate neuroprotective effects of new skin care formulations against UV exposure damage.
  
  
  METHODS
  Samples were obtained from 34 female patients enrolled for plastic surgery and were immediately treated (10 min) with three emulsions: Cream 1, Cream 2 (placebo) and a sunscreen with sun protection factor 15 (SPF15). Control samples and those treated with the cream emulsions were exposed to UVA and UVB for 60 min. Nerve fibres were identified by immunofluorescence using a monoclonal antibody (anti-human CD56/NCAM). Cell damage was assessed by image analysis.
  
  
  RESULTS
  Several cellular nervous structures were identified in the skin samples, including free nerve endings. UVA and UVB significantly decreased (40-60%) density of nerve endings in the control samples and those treated with placebo (Cream 2) or SPF15 (all P < 0.001). Cream 1 completely blocked effects of UV radiation on nerve endings (P > 0.05 vs. control).
  
  
  CONCLUSIONS
  Quantification of cell damage induced by UV radiation provides useful information for identification of new skin care compounds with neuroprotective properties.
authors.affiliation: Institute of Biomedical Research, Hospital São Lucas, RS, Brazil.
authors.forename: A L
authors.initials: AL
authors.lastname: Rodriguez
authors.forename: F S
authors.initials: FS
authors.lastname: Stefani
authors.forename: C E
authors.initials: CE
authors.lastname: de Oliveira Praes
authors.forename: A
authors.initials: A
authors.lastname: Piaceski
authors.forename: M P
authors.initials: MP
authors.lastname: Oliveira
authors.forename: P
authors.initials: P
authors.lastname: Martins
authors.forename: V D
authors.initials: VD
authors.lastname: da Silva
authors.forename: C
authors.initials: C
authors.lastname: Bonorino
authors.forename: M E
authors.initials: ME
authors.lastname: Bauer
mesh_terms: D000328:Adult; D005260:Female; D005455:Fluorescent Antibody Technique; D006801:Humans; D008875:Middle Aged; D009412:Nerve Fibers / Q000187:drug effects* / Q000473:pathology / Q000528:radiation effects*; D012867:Skin / Q000187:drug effects* / Q000473:pathology / Q000528:radiation effects*; D013473:Sunscreening Agents / Q000627:therapeutic use; D014466:Ultraviolet Rays / Q000009:adverse effects*; D055815:Young Adult
chemical_list: D013473:Sunscreening Agents