The first virally encoded cytochrome p450.
The genome sequence of the giant virus Acanthamoeba polyphaga mimivirus revealed the presence of two putative cytochrome P450 (CYP) genes. The product of one of the two predicted CYP genes (YP_143162) showed low-level homology to sterol 14-demethylase (CYP51) and contained a C-terminal polypeptide domain of unknown function. YP_143162 expression (without an N-terminal membrane binding domain) in Escherichia coli yields a CYP protein which gives a reduced CO difference maximum at 448 nm and was formally demonstrated as the first viral cytochrome P450. Analysis of binding of lipid and sterol substrates indicated no perturbation in CYP heme environment, and an absence of activity was seen when 14-methyl sterols were used as a substrate. The function of the CYP protein and its C-terminal domain remain unknown.
Lamb
David C
DC
Institute of Life Science, Swansea University, United Kingdom. d.c.lamb@swansea.ac.uk
Lei
Li
L
Warrilow
Andrew G S
AG
Lepesheva
Galina I
GI
Mullins
Jonathan G L
JG
Waterman
Michael R
MR
Kelly
Steven L
SL
D000048:Acanthamoeba / Q000821:virology*; D000595:Amino Acid Sequence; D000818:Animals; D003577:Cytochrome P-450 Enzyme System / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D004267:DNA Viruses / Q000737:chemistry / Q000145:classification / Q000201:enzymology* / Q000235:genetics; D008968:Molecular Conformation; D008969:Molecular Sequence Data; D010802:Phylogeny; D016415:Sequence Alignment; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism
D014764:Viral Proteins; D003577:Cytochrome P-450 Enzyme System