title: Myeloperoxidase concentrations in EDTA-plasma of healthy subjects are discordant with concentrations in heparin-plasma and serum.
abstract: OBJECTIVE
  To examine the effect of blood anticoagulation type on the myeloperoxidase (MPO) concentration.
  
  
  METHODS
  MPO was measured in EDTA-plasma and matched heparin-plasma and serum samples collected from healthy volunteers.
  
  
  RESULTS
  MPO concentrations in heparin-plasma and serum were higher than in EDTA-plasma (both P<0.001). MPO in EDTA-plasma was not significantly correlated with MPO in either heparin-plasma or serum.
  
  
  CONCLUSIONS
  For MPO measurements, EDTA-plasma is the preferred specimen as it appears unaffected by ex vivo release of MPO from leukocytes.
authors.affiliation: Metabolic Laboratory, Department of Clinical Chemistry, VU University Medical Center, P.O. Box 7057, 1007 MB Amsterdam, The Netherlands. p.scheffer@vumc.nl
authors.forename: Peter G
authors.initials: PG
authors.lastname: Scheffer
authors.forename: Leonard P
authors.initials: LP
authors.lastname: van der Zwan
authors.forename: Roger K
authors.initials: RK
authors.lastname: Schindhelm
authors.forename: Hendrikus P A
authors.initials: HP
authors.lastname: Vermue
authors.forename: Tom
authors.initials: T
authors.lastname: Teerlink
mesh_terms: D000328:Adult; D000925:Anticoagulants / Q000494:pharmacology*; D001793:Blood Preservation / Q000379:methods; D004492:Edetic Acid / Q000494:pharmacology; D005260:Female; D006493:Heparin / Q000494:pharmacology; D006801:Humans; D008297:Male; D009195:Peroxidase / Q000097:blood*; D010949:Plasma / Q000187:drug effects* / Q000201:enzymology; D044967:Serum / Q000187:drug effects* / Q000201:enzymology; D013696:Temperature
chemical_list: D000925:Anticoagulants; D006493:Heparin; D004492:Edetic Acid; D009195:Peroxidase