title: A new microdialysis-electrochemical device for in vivo simultaneous determination of acetylcholine and choline in rat brain treated with N-methyl-(R)-salsolinol.
abstract: Acetylcholine (ACh) and choline (Ch) play a critical role in cholinergic neurotransmission and the abnormalities in their concentrations are related to several neural diseases. Therefore, the in vivo determination of ACh and Ch is important to the research on neurodegenerative disorders. In this work, electrochemical biosensors based on poly(m-(1,3)-phenylenediamine) (pmPD) and polytyramine (PTy) modified enzyme electrodes were fabricated. The electropolymerized pmPD polymer was used to exclude interfering substances and the PTy layer facilitated the immobilization of acetylcholinesterase (AChE) and choline oxidase (ChOx). Then, ACh/Ch sensor and Ch sensor were coupled with microdialysis to produce a novel device, which provides a sensitive and selective method for simultaneous determination of ACh and Ch. This method has detection limits of 63.0+/-3.4 nM for ACh and 25.0+/-1.2 nM for Ch. The integrated device was successfully applied to assessing the impact of endogenous neurotoxin N-methyl-(R)-salsolinol [1(R),2-dimethyl-6,7-dihydroxy-1,2,3,4-tetrahydroisoquinoline, (R)-NMSal] on ACh and Ch concentration, which is of great benefit to understand the pathogenesis of Parkinson's disease.
authors.affiliation: Department of Chemistry, East China Normal University, 3663 Zhongshan Rd.(N), Shanghai 200062, PR China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhu
authors.forename: Yarui
authors.initials: Y
authors.lastname: An
authors.forename: Jiaohong
authors.initials: J
authors.lastname: Zheng
authors.forename: Linlin
authors.initials: L
authors.lastname: Tang
authors.forename: Wen
authors.initials: W
authors.lastname: Zhang
authors.forename: Litong
authors.initials: L
authors.lastname: Jin
authors.forename: Lai
authors.initials: L
authors.lastname: Jiang
mesh_terms: D000109:Acetylcholine / Q000032:analysis*; D000818:Animals; D015374:Biosensing Techniques / Q000295:instrumentation; D001921:Brain / Q000187:drug effects* / Q000378:metabolism*; D002794:Choline / Q000032:analysis*; D004563:Electrochemistry / Q000295:instrumentation*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D008297:Male; D017551:Microdialysis / Q000295:instrumentation*; D010302:Parkinson Disease, Secondary / Q000139:chemically induced / Q000175:diagnosis / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012490:Salsoline Alkaloids
chemical_list: D012490:Salsoline Alkaloids; C032837:salsoline; D002794:Choline; D000109:Acetylcholine