Retrieval of immature oocytes after chemotherapy for Hodgkin's disease and prolonged ovarian down-regulation with gonadotropin-releasing hormone agonist.
OBJECTIVE
To describe isolation and in vitro maturation of primary oocytes from the ovarian cortex in the presence of hypothalamic pituitary down-regulation.


METHODS
Case report.


METHODS
Tertiary care university-affiliated hospital.


METHODS
An 18-year-old patient was given treatment with the ABVD (Adriamycin, bleomycin, vinblastine, and dacarbazine) protocol for Hodgkin's disease. She underwent ovarian tissue cryopreservation while being cotreated with GnRH agonist because of disease relapse.


METHODS
Laparoscopic oophorectomy, ovarian tissue cryopreservation, and in vitro maturation of primary oocytes.


METHODS
Maturation of primary oocytes isolated from the medium used for preparation of ovarian tissue.


RESULTS
Twenty-one immature germinal vesicle-stage oocytes were isolated from the medium of dissection. All were incubated in in vitro maturation medium, and five were maturated and frozen.


CONCLUSIONS
The fact that germinal vesicle-stage oocytes were present in our patient's medium despite hormonal down-regulation demonstrates that GnRH agonist does not completely inhibit antral follicle development.
Azem
Foad
F
Racine In Vitro Fertilization Unit, Lis Maternity Hospital, Tel Aviv Sourasky Medical Center, Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv, Israel. azemf@tasmc.health.gov.il
Hasson
Joseph
J
Cohen
Tanya
T
Shwartz
Tamar
T
Mey-Raz
Nava
N
Almog
Beni
B
Amit
Ami
A
Ben-Yosef
Dalit
D
D000328:Adult; D000970:Antineoplastic Agents / Q000008:administration & dosage*; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D015536:Down-Regulation / Q000187:drug effects; D004334:Drug Administration Schedule; D005260:Female; D005300:Fertility Agents, Female / Q000008:administration & dosage*; D007987:Gonadotropin-Releasing Hormone / Q000819:agonists*; D006689:Hodgkin Disease / Q000188:drug therapy* / Q000473:pathology*; D006801:Humans; D007247:Infertility, Female / Q000628:therapy*; D054315:Oocyte Retrieval / Q000379:methods*; D010062:Ovulation Induction / Q000379:methods*; D012189:Retrospective Studies; D016896:Treatment Outcome
D000970:Antineoplastic Agents; D005300:Fertility Agents, Female; D007987:Gonadotropin-Releasing Hormone