Phage display selection of cyclic peptides that inhibit Andes virus infection.
Specific therapy is not available for hantavirus cardiopulmonary syndrome caused by Andes virus (ANDV). Peptides capable of blocking ANDV infection in vitro were identified using antibodies against ANDV surface glycoproteins Gn and Gc to competitively elute a cyclic nonapeptide-bearing phage display library from purified ANDV particles. Phage was examined for ANDV infection inhibition in vitro, and nonapeptides were synthesized based on the most-potent phage sequences. Three peptides showed levels of viral inhibition which were significantly increased by combination treatment with anti-Gn- and anti-Gc-targeting peptides. These peptides will be valuable tools for further development of both peptide and nonpeptide therapeutic agents.
Hall
Pamela R
PR
Department of Pathology, University of New Mexico Health Sciences Center, Albuquerque, New Mexico 87131, USA.
Hjelle
Brian
B
Njus
Hadya
H
Ye
Chunyan
C
Bondu-Hawkins
Virginie
V
Brown
David C
DC
Kilpatrick
Kathleen A
KA
Larson
Richard S
RS
D000595:Amino Acid Sequence; D000818:Animals; D000998:Antiviral Agents / Q000138:chemical synthesis / Q000302:isolation & purification* / Q000494:pharmacology*; D002522:Chlorocebus aethiops; D006476:Orthohantavirus / Q000187:drug effects*; D006801:Humans; D008826:Microbial Sensitivity Tests; D008958:Models, Molecular; D008969:Molecular Sequence Data; D019151:Peptide Library; D010456:Peptides, Cyclic / Q000138:chemical synthesis / Q000235:genetics / Q000302:isolation & purification* / Q000494:pharmacology*; D014709:Vero Cells
D000998:Antiviral Agents; D019151:Peptide Library; D010456:Peptides, Cyclic