Fluorescence quenching properties of multiple pyrene-modified RNAs.
RNA molecules with multiple pyrenylmethyl substituents on the 2'-O-sugar residues can form duplexes with complementary RNA sequences without losing thermal stability. In the RNA duplexes, covalently incorporated pyrenes can assemble in a helical manner along the minor grooves of the duplex. These helically assembled pyrene arrays exhibit intense excimer emissions that are efficiently quenched with methyl viologen.
Maie
Kenji
K
Department of Materials Science and Chemistry, Graduate School of Engineering, University of Hyogo, 2167 Shosha, Himeji, Hyogo 671-2280, Japan.
Nakamura
Mitsunobu
M
Takada
Tadao
T
Yamana
Kazushige
K
D001483:Base Sequence; D005453:Fluorescence; D008697:Methane / Q000031:analogs & derivatives / Q000737:chemistry; D008958:Models, Molecular; D009690:Nucleic Acid Conformation; D010269:Paraquat / Q000737:chemistry*; D011721:Pyrenes / Q000737:chemistry*; D012313:RNA / Q000737:chemistry*; D020871:RNA Stability; D044366:Transition Temperature
D011721:Pyrenes; C051224:methyl radical; D012313:RNA; C030984:pyrene; D008697:Methane; D010269:Paraquat