title: [Clinical application of avidin-biotin ELISA to detect serum hepatoma-specific gamma-glutamyltransferase in patients with primary hepatic cancer].
abstract: OBJECTIVE
  To detect serum hepatoma-specific datura stramonium lectin-tightly binding gamma-glutamyl transferase (DSA-GGT) in patients with primary hepatic cancer (PHC) by avidin-biotin ELISA method which was established in our laboratory, and carry on a study of its clinical application.
  
  
  METHODS
  To detect serum DSA-GGT in 45 healthy control subjects, 58 PHC patients and 203 non-PHC patients (including 36 patients with other tumors and 167 patients with benign liver diseases) with the method was established; meanwhile, AFP was detected by ELISA method.
  
  
  RESULTS
  38 individuals were DSA-GGT positive in 58 PHC patients, the sensitivity was 65.5%. 18 individuals were DSA-GGT positive in 203 patients without PHC, the specificity was 91.1%. The sensitivity and specificity of AFP in diagnosis of PHC patients was 69.0% and 90.6%, respectively. The sensitivity and specificity of combination of DSA-GGT and AFP was 93.1% and 85.7%, respectively. The average intra-CV and inter-CV of DSA-GGT ELISA was 8.9% and 11.5%, respectively.
  
  
  CONCLUSIONS
  The sensitivity and specificity of DSA-GGT ELISA method established in our lab is similar with that of AFP assay and the accuracy is good. Combination of DSA-GGT and AFP may improve the diagnostic sensitivity. The method should be potentially as a new way to improve diagnosis of PHC.
authors.affiliation: Laboratory Center of Hepatic Diseases, Nanjing Second Hospital Affiliated to Southeast University Medical College, Nanjing 210003, China.
authors.forename: Nian-yue
authors.initials: NY
authors.lastname: Wang
authors.forename: Wei
authors.initials: W
authors.lastname: Zhao
authors.forename: Dai
authors.initials: D
authors.lastname: Zhang
authors.forename: Yong-cheng
authors.initials: YC
authors.lastname: Zhang
authors.forename: Mu-hao
authors.initials: MH
authors.lastname: Duan
mesh_terms: D000328:Adult; D001360:Avidin; D001710:Biotin; D006528:Carcinoma, Hepatocellular / Q000097:blood / Q000175:diagnosis* / Q000201:enzymology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005260:Female; D006801:Humans; D008113:Liver Neoplasms / Q000097:blood / Q000175:diagnosis* / Q000201:enzymology; D008297:Male; D008875:Middle Aged; D012680:Sensitivity and Specificity; D055815:Young Adult; D000509:alpha-Fetoproteins / Q000032:analysis; D005723:gamma-Glutamyltransferase / Q000097:blood*
chemical_list: D000509:alpha-Fetoproteins; D001360:Avidin; D001710:Biotin; D005723:gamma-Glutamyltransferase