[Immunomorphology in the diagnosis of orbital rhabdomyosarcoma].
Immunohistochemistry (IHC) using monoclonal antibodies is presently the most reliable method to verify tumor tissue belonging. The histological diagnosis of rhabdomyosarcoma (RMS) was immunohistochemically confirmed in 7 of 10 orbital neoplasms in children. In 3 cases the diagnosis of RMS was established after IHC study that revealed the atypical tumor cell expression of the muscular antigens--myoglobin, protein Myo D1, actin HHF-35, which is indicative of the rhabdomyogenic genesis of a tumor. IHC data indicated that, no matter what the histological pattern, orbital RMS is characterized by the expression of cytospecific proteins--myoglobin, Myo D1, actin HHF-35, and tissue-specific (intermediate filaments)--vimentin and desmin. The use of appropriate antibodies offers a means of making a histogenetic diagnosis even in cases of the atypical morphological pattern.
Andreeva
L D
LD
Khoroshilova-Maslova
I P
IP
D000293:Adolescent; D000912:Antibodies, Neoplasm / Q000032:analysis*; D014408:Biomarkers, Tumor / Q000276:immunology / Q000378:metabolism; D002648:Child; D002675:Child, Preschool; D003937:Diagnosis, Differential; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods*; D007223:Infant; D017570:MyoD Protein / Q000276:immunology / Q000378:metabolism*; D009211:Myoglobin / Q000276:immunology / Q000378:metabolism*; D009918:Orbital Neoplasms / Q000276:immunology / Q000378:metabolism / Q000473:pathology*; D012208:Rhabdomyosarcoma / Q000276:immunology / Q000378:metabolism / Q000473:pathology*
D000912:Antibodies, Neoplasm; D014408:Biomarkers, Tumor; D017570:MyoD Protein; C057363:MyoD1 myogenic differentiation protein; D009211:Myoglobin