No association of the NFKB1 insertion/deletion promoter polymorphism with survival in patients with gastric cancer.
OBJECTIVE
The present study analyzed the functional insertion/deletion polymorphism in the promoter region of NFKB1 gene and their impact on the prognosis for patients with gastric adenocarcinoma.


METHODS
Four hundred and seventy two consecutive patients with curatively resected gastric adenocarcinoma were enrolled in the present study. The genomic DNA was extracted from paraffin-embedded tissue and the -94 insertion/deletion ATTG polymorphism of NFKB1 determined using a polymerase chain reaction-restriction fragment length polymorphism assay.


RESULTS
The NFKB1 promoter gene polymorphism was successfully amplified in 97.8% of the cases. There were no sexual differences in relation to the genotype and allele. No correlation was observed between the frequency of the genotype or allele and the T, N or M stage. The multivariate survival analysis showed no association between the NFKB1 -94 insertion/deletion promoter polymorphism and the disease-free survival or overall survival of the patients with gastric cancer.


CONCLUSIONS
The functional NFKB1 promoter polymorphism was not found to be a prognostic marker for Korean patients with surgically resected gastric adenocarcinoma.
Kim
Jong Gwang
JG
Department of Surgery, Kyungpook National University Hospital, Kyungpook National University School of Medicine, 50 Samduck 2-Ga, Jung-Gu, Daegu 700-712, Korea. wyu@mail.knu.ac.kr
Sohn
Sang Kyun
SK
Chae
Yee Soo
YS
Moon
Joon Ho
JH
Kim
Shi Nae
SN
Kang
Byung Woog
BW
Kim
Gab Chul
GC
Lee
Myung-Hoon
MH
Jeon
Seong Woo
SW
Chung
Ho Young
HY
Yu
Wansik
W
D000230:Adenocarcinoma / Q000235:genetics / Q000401:mortality / Q000556:secondary; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D004273:DNA, Neoplasm / Q000235:genetics / Q000378:metabolism; D005240:Feasibility Studies; D005260:Female; D017353:Gene Deletion; D006801:Humans; D007723:Korea; D008297:Male; D008875:Middle Aged; D016254:Mutagenesis, Insertional; D052002:NF-kappa B p50 Subunit / Q000235:genetics*; D009367:Neoplasm Staging; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D011379:Prognosis; D011401:Promoter Regions, Genetic / Q000235:genetics*; D013274:Stomach Neoplasms / Q000235:genetics* / Q000401:mortality* / Q000473:pathology; D015996:Survival Rate; D016896:Treatment Outcome
D004273:DNA, Neoplasm; D052002:NF-kappa B p50 Subunit; C489548:NFKB1 protein, human