title: Staurosporine-induced apoptosis in P388D1 macrophages involves both extrinsic and intrinsic pathways.
abstract: Treatment of P388D1, a macrophage-like cell line, with staurosporine triggered apoptosis through the activation of caspase-9 and caspase-3. Unexpected effects of staurosporine on the induction of apoptosis were the activation of caspase-8, and an increase of the levels of TNF-alpha. The increased TNF-alpha levels led to activation of caspase-8 by an autocrine effect via the TNF receptor expressed by the P388D1 macrophages. In contrast, P388D1 macrophages that either had been exposed to UV light or treated with dexamethasone did not undergo apoptosis.
authors.affiliation: Laboratory of Virology, Department of Microbiology and Parasitology, Universidad Nacional Autónoma de México,Ciudad Universitaria, México DF 04510, Mexico.
authors.forename: Yuko
authors.initials: Y
authors.lastname: Nakamura-López
authors.forename: Rosa Elena
authors.initials: RE
authors.lastname: Sarmiento-Silva
authors.forename: Julio
authors.initials: J
authors.lastname: Moran-Andrade
authors.forename: Beatriz
authors.initials: B
authors.lastname: Gómez-García
mesh_terms: D000818:Animals; D018931:Antineoplastic Agents, Hormonal / Q000494:pharmacology; D017209:Apoptosis; D053148:Caspase 3 / Q000187:drug effects / Q000378:metabolism; D053181:Caspase 8 / Q000187:drug effects / Q000378:metabolism; D053453:Caspase 9 / Q000187:drug effects / Q000378:metabolism; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects / Q000502:physiology; D053938:DNA Fragmentation / Q000187:drug effects; D003907:Dexamethasone / Q000494:pharmacology; D008264:Macrophages / Q000187:drug effects* / Q000528:radiation effects; D051379:Mice; D047428:Protein Kinase Inhibitors / Q000494:pharmacology*; D047888:Receptors, Tumor Necrosis Factor, Type I / Q000819:agonists / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects / Q000502:physiology; D019311:Staurosporine / Q000494:pharmacology*; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis / Q000187:drug effects; D014466:Ultraviolet Rays / Q000009:adverse effects
chemical_list: D018931:Antineoplastic Agents, Hormonal; D047428:Protein Kinase Inhibitors; D047888:Receptors, Tumor Necrosis Factor, Type I; D014409:Tumor Necrosis Factor-alpha; D003907:Dexamethasone; D053148:Caspase 3; D053181:Caspase 8; D053453:Caspase 9; D019311:Staurosporine