Antigenotoxic and antioxidant activities of isorhamnetin 3-O neohesperidoside from Acacia salicina.
Antioxidant activity of isorhamnetin 3-O neohesperidoside (I3ON), isolated from the leaves of Acacia salicina, was determined by the ability of this compound to inhibit lipid peroxidation and to protect against hydroxyl radical-induced DNA damage in pKS plasmid DNA and Escherichia coli cultures. Antigenotoxic activity was assessed by using the comet assay. The IC(50) value of the inhibitory activity toward lipid peroxidation by I3ON is 0.6 mM. This compound was also able to protect against hydroxyl radical-induced DNA damage in pKS plasmid DNA. Moreover, this compound induced an inhibitory activity toward H2O2-induced genotoxicity. The protective effect exhibited by this molecule was also determined by analysis of gene expression as a response to an oxidative stress, using a cDNA microarray. Transcription of several genes related to the antioxidant system (HMOX2 and TXNL) and to the DNA repair pathway (XPC, POLD1, POLD2, PCNA, DDIT3, APEX, and LIG4) were upregulated after incubation with I3ON. Taken together, these observations provide evidence that the I3ON, isolated from the leaves of A. salicina, is able to protect cells against oxidative stress.
Bouhlel
Ines
I
Unité de Pharmacognosie/Biologie Moléculaire 99/UR/07-03, Faculté de Pharmacie/Médecine Dentaire de Monastir, Rue Avicenne, 5000 Monastir, Tunisie.
Skandrani
Ines
I
Nefatti
Aicha
A
Valenti
Kita
K
Ghedira
Kamel
K
Mariotte
Anne Marie
AM
Hininger-Favier
Isabelle
I
Laporte
Francois
F
Dijoux-Franca
Marie G
MG
Chekir-Ghedira
Leila
L
D000045:Acacia / Q000737:chemistry*; D016587:Antimutagenic Agents / Q000494:pharmacology*; D000975:Antioxidants / Q000494:pharmacology*; D045744:Cell Line, Tumor; D020552:Comet Assay; D004249:DNA Damage; D004260:DNA Repair / Q000187:drug effects / Q000235:genetics; D044948:Flavonols / Q000494:pharmacology; D015870:Gene Expression / Q000187:drug effects; D006801:Humans; D006861:Hydrogen Peroxide / Q000633:toxicity; D017665:Hydroxyl Radical / Q000633:toxicity; D015227:Lipid Peroxidation / Q000187:drug effects; D020411:Oligonucleotide Array Sequence Analysis; D016877:Oxidants / Q000633:toxicity; D018384:Oxidative Stress / Q000187:drug effects* / Q000235:genetics; D018515:Plant Leaves / Q000737:chemistry; D010957:Plasmids / Q000187:drug effects / Q000235:genetics; D015854:Up-Regulation / Q000187:drug effects
D016587:Antimutagenic Agents; D000975:Antioxidants; D044948:Flavonols; D016877:Oxidants; C469309:isorhamnetin-3-O-neohesperidoside; D017665:Hydroxyl Radical; D006861:Hydrogen Peroxide