title: Immunohistochemical expression of basement membrane proteins of verrucous carcinoma of the oral mucosa.
abstract: Squamous cell carcinoma (SCC) of the oral cavity is an extremely invasive tumour of stratified squamous epithelium that spreads throughout degradation of the basement membrane (BM) and extra-cellular matrix. Oral verrucous carcinoma (VC) is a rare low-grade variant of oral SCC that penetrates into the subepithelial connective tissue. It also has a different clinical behaviour from classical oral SCC. We investigated the immunohistochemical expression of laminin, laminin-5, collagen IV and fibronectin in VC, severe epithelial dysplasia (SED) and SCC in order to analyse if the pattern of these molecules expression contributes to the differences in the biological behaviour of these diseases. The staining pattern of laminin was less intensive in SCC compared with SED and VC, and collagen IV expression was increased in VC compared with SED. Discontinuities of laminin, collagen IV and fibronectin were more evident in SED than in VC. This study indicates that VC has a biological behaviour different from SED or SCC, observable by immunohistochemistry in the BM zone.
authors.affiliation: Division of Otorhinolaryngology, Department of Clinical Physiopathology, Oral Medicine Section, University of Turin, Unito Lingotto Dental Institute, Via Nizza 230, 10126 Turin, Italy. paolo.arduino@gmail.com
authors.forename: Paolo G
authors.initials: PG
authors.lastname: Arduino
authors.forename: Marco
authors.initials: M
authors.lastname: Carrozzo
authors.forename: Marco
authors.initials: M
authors.lastname: Pagano
authors.forename: Roberto
authors.initials: R
authors.lastname: Broccoletti
authors.forename: Crispian
authors.initials: C
authors.lastname: Scully
authors.forename: Sergio
authors.initials: S
authors.lastname: Gandolfo
mesh_terms: D000368:Aged; D001485:Basement Membrane / Q000737:chemistry*; D002278:Carcinoma in Situ / Q000473:pathology; D002294:Carcinoma, Squamous Cell / Q000473:pathology; D018289:Carcinoma, Verrucous / Q000473:pathology*; D015815:Cell Adhesion Molecules / Q000032:analysis*; D024141:Collagen Type IV / Q000032:analysis; D004396:Coloring Agents; D003238:Connective Tissue / Q000473:pathology; D004848:Epithelium / Q000473:pathology; D016326:Extracellular Matrix Proteins / Q000032:analysis*; D005260:Female; D005353:Fibronectins / Q000032:analysis; D006801:Humans; D007150:Immunohistochemistry; D007797:Laminin / Q000032:analysis; D008297:Male; D009062:Mouth Neoplasms / Q000473:pathology*; D000097794:Kalinin
chemical_list: D015815:Cell Adhesion Molecules; D024141:Collagen Type IV; D004396:Coloring Agents; D016326:Extracellular Matrix Proteins; D005353:Fibronectins; D007797:Laminin; D000097794:Kalinin