title: TOE1 interacts with p53 to modulate its transactivation potential.
abstract: The TOE1 gene was discovered as a target of the Egr1 transcription factor that participates in cell growth regulation through the upregulation of p21 and a cell cycle delay at the G2/M phase. We report here that TOE1 is able to bind to the p53 tumor suppressor protein, specifically interacting with the C terminal tetramerization domain of p53. We have further characterized this interaction through determination of binding kinetics using nanoporous optical interferometry and demonstrated that this interaction is capable of enhancing the transcriptional activity of p53-dependent gene targets. These results suggest a mechanistic role for TOE1 as a co-regulator of p53.
authors.affiliation: Centre de Recherche du CHUL, Department of Molecular Endocrinology and Oncology, Laval University, Quebec, QC Canada.
authors.forename: Sabina
authors.initials: S
authors.lastname: Sperandio
authors.forename: Saverio
authors.initials: S
authors.lastname: Tardito
authors.forename: Aleksandra
authors.initials: A
authors.lastname: Surzycki
authors.forename: Martin
authors.initials: M
authors.lastname: Latterich
authors.forename: Ian
authors.initials: I
authors.lastname: de Belle
mesh_terms: D001665:Binding Sites; D002478:Cells, Cultured; D006367:HeLa Cells; D006801:Humans; D007700:Kinetics; D009687:Nuclear Proteins / Q000378:metabolism*; D017434:Protein Structure, Tertiary; D015533:Transcriptional Activation; D016159:Tumor Suppressor Protein p53 / Q000235:genetics* / Q000378:metabolism
chemical_list: D009687:Nuclear Proteins; C474027:TOE1 protein, human; D016159:Tumor Suppressor Protein p53