title: An experimental investigation on aspects of infectious salmon anaemia virus (ISAV) infection dynamics in seawater Atlantic salmon, Salmo salar L.
abstract: This study investigated infection dynamics of infectious salmon anaemia virus (ISAV) by conducting two experiments to examine minimum infective dose and viral shedding of ISAV. In terms of minimum infective dose, the high variability between replicate tanks and the relatively slow spread of infection through the population at 1 x 10(1) TCID(50) mL(-1) indicated this dose is approaching the minimum infective dose for ISAV in seawater salmon populations. A novel qPCR assay incorporating an influenza virus control standard with each seawater sample was developed that enabled the quantity of ISAV shed from infected populations to be estimated in values equivalent to viral titres. Viral shedding was first detected at 7 days post-challenge (5.8 x 10(-2) TCID(50) mL(-1)kg(-1)) and rose to levels above the minimum infective dose (4.2 x 10(1) TCID(50) mL(-1)kg(-1)) on day 11 post-challenge, 2 days before mortalities in ISAV inoculated fish started. These results clearly demonstrate that a large viral shedding event occurs before death. Viral titres peaked at 7.0 x 10(1) TCID(50) mL(-1)kg(-1) 15 days post-infection. These data provide important information relevant to the management of ISA.
authors.affiliation: Fisheries Research Services, Aberdeen, UK.
authors.forename: A
authors.initials: A
authors.lastname: Gregory
authors.forename: L A
authors.initials: LA
authors.lastname: Munro
authors.forename: M
authors.initials: M
authors.lastname: Snow
authors.forename: K L
authors.initials: KL
authors.lastname: Urquhart
authors.forename: A G
authors.initials: AG
authors.lastname: Murray
authors.forename: R S
authors.initials: RS
authors.lastname: Raynard
mesh_terms: D000818:Animals; D005393:Fish Diseases / Q000635:transmission* / Q000821:virology*; D046829:Isavirus; D009841:Oligonucleotides / Q000235:genetics; D009976:Orthomyxoviridae Infections / Q000635:transmission / Q000662:veterinary*; D016133:Polymerase Chain Reaction / Q000662:veterinary; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D020317:Salmo salar; D012623:Seawater; D017201:Virus Shedding / Q000502:physiology*
chemical_list: D009841:Oligonucleotides