Second harmonic imaging and scoring of collagen in fibrotic tissues.
We compare second harmonic generation (SHG) to histological and immunohistochemical techniques for the visualization and scoring of collagen in biological tissues. We show that SHG microscopy is highly specific for fibrillar collagens and that combined SHG and two-photon excited fluorescence (2PEF) imaging can provide simultaneous three-dimensional visualization of collagen synthesis and assembly sites in transgenic animal models expressing GFP constructs. Finally, we propose several scores for characterizing collagen accumulation based on SHG images and appropriate for different types of collagen distributions. We illustrate the sensitivity of these scores in a murine model of renal fibrosis using a morphological segmentation of the tissue based on endogenous 2PEF signals.
Strupler
M
M
Pena
A-M
AM
Hernest
M
M
Tharaux
P-L
PL
Martin
J-L
JL
Beaurepaire
E
E
Schanne-Klein
M-C
MC