Optimized nutrient medium for galanthamine production in Leucojum aestivum L. in vitro shoot system.
The common effect of NH4+, NO3-, KH2PO4 and sucrose on the biosynthesis of galanthamine by a Leucojum aestivum shoot culture was studied. Polynominal regression models were elaborated for the description of the galanthamine biosynthesis as a consequence of variation of the investigated variables (NH4+ between 0.20 and 0.54 g/L; NO3- between 1.44 and 3.44 g/L; KH2PO4 between 0.10 and 0.24 g/L, and sucrose between 30.00 and 60.00 g/L). Optimization procedures allowed us to establish the optimal concentrations of the investigated variables and to propose the modified MS nutrient medium, with 4.50 g/L KNO3, 0.89 g/L NH4NO3, 1.25 g/L (NH4)2SO4, 0.10 g/L KH2PO4 and 60 g/L sucrose, for the galanthamine production by a Leucojum aestivum shoot culture. The proposed modified MS medium provided considerable increase of both the production yield and the relative content of the target alkaloid in the alkaloid mixture.
Georgiev
Vasil
V
Department of Microbial Biosynthesis and Biotechnologies, Laboratory in Plovdiv, The Stephan Angeloff Institute of Microbiology, Bulgarian Academy of Sciences, 26 Maritza Blvd., 4002 Plovdiv, Bulgaria.
Berkov
Strahil
S
Georgiev
Milen
M
Burrus
Monique
M
Codina
Carles
C
Bastida
Jaume
J
Ilieva
Mladenka
M
Pavlov
Atanas
A
D000470:Alkaloids / Q000096:biosynthesis / Q000737:chemistry; D018533:Biomass; D003470:Culture Media; D005702:Galantamine / Q000096:biosynthesis* / Q000737:chemistry / Q000302:isolation & purification; D008401:Gas Chromatography-Mass Spectrometry; D007700:Kinetics; D019668:Liliaceae / Q000378:metabolism*; D018520:Plant Shoots / Q000378:metabolism*
D000470:Alkaloids; D003470:Culture Media; D005702:Galantamine