Vaccination with EtpA glycoprotein or flagellin protects against colonization with enterotoxigenic Escherichia coli in a murine model.
Enterotoxigenic Escherichia coli (ETEC) remain a leading cause diarrheal illness, prompting a search for vaccine targets that led to the recent discovery of EtpA, a secreted adhesin of ETEC that acts by bridging flagella and host cells. In a murine model, immunization with recombinant EtpA glycoprotein inhibited colonization by two EtpA-producing human ETEC strains, H10407 and E24377A. In addition, vaccination with recombinant flagellin (serotype H11) generated antibodies that specifically recognized the tips of flagella from E24377A expressing a heterologous flagellar serotype (H28) and afforded significant protection against colonization. EtpA and/or flagellin could be valuable subunit antigens in the formulation of a broadly protective ETEC vaccine.
Roy
Koushik
K
Veterans Affairs Medical Center, Departments of Medicine, University of Tennessee Health Science Center, Memphis, TN, USA.
Hamilton
David
D
Ostmann
Marguerite M
MM
Fleckenstein
James M
JM
D000595:Amino Acid Sequence; D000818:Animals; D015169:Colony Count, Microbial; D054307:Enterotoxigenic Escherichia coli / Q000276:immunology*; D029968:Escherichia coli Proteins / Q000276:immunology*; D022361:Escherichia coli Vaccines / Q000276:immunology*; D005408:Flagellin / Q000276:immunology*; D007421:Intestine, Small / Q000382:microbiology; D008562:Membrane Glycoproteins / Q000276:immunology*; D051379:Mice; D008813:Mice, Inbred ICR; D008969:Molecular Sequence Data; D016415:Sequence Alignment; D022223:Vaccines, Subunit / Q000276:immunology; D014614:Vaccines, Synthetic / Q000276:immunology
D029968:Escherichia coli Proteins; D022361:Escherichia coli Vaccines; C510011:EtpA protein, E coli; D008562:Membrane Glycoproteins; D022223:Vaccines, Subunit; D014614:Vaccines, Synthetic; D005408:Flagellin