Tetra primer ARMS-PCR for identification of SNP in beta-tubulin of Botrytis cinerea, responsible of resistance to benzimidazole.
A competitive polymerase chain reaction (PCR) technique called Tetra primer amplification refractory mutation system (ARMS) PCR was adapted to identify a single nucleotide polymorphism (SNP) in beta-tubulin gene of Botrytis cinerea. Over the 35 isolates analyzed, six of them showed a SNP in that gene, which was readily identified by the technique and in the six cases correspond to the resistant strain.
Muñoz
Claudio
C
Plant Virology Laboratory, EEA Mendoza INTA, San Martín 3853, Luján de Cuyo (5507), Mendoza, Argentina.
Gómez Talquenca
Sebastián
S
Volpe
Melisa Lanza
ML
D000935:Antifungal Agents / Q000494:pharmacology*; D001562:Benzimidazoles / Q000494:pharmacology*; D020171:Botrytis / Q000187:drug effects / Q000235:genetics*; D017931:DNA Primers / Q000235:genetics; D004271:DNA, Fungal / Q000235:genetics; D025141:Drug Resistance, Fungal; D016133:Polymerase Chain Reaction / Q000379:methods*; D020641:Polymorphism, Single Nucleotide; D014404:Tubulin / Q000235:genetics*
D000935:Antifungal Agents; D001562:Benzimidazoles; D017931:DNA Primers; D004271:DNA, Fungal; D014404:Tubulin; C031000:benzimidazole