title: Microfabricating high-aspect-ratio structures in polyurethane-methacrylate (PUMA) disposable microfluidic devices.
abstract: We recently reported a new UV-curable polyurethane-methacrylate (PUMA) resin that has excellent qualities as a disposable microfluidic substrate for clinical diagnostic applications. This article discusses strategies to improve the production yield of PUMA chips that contain dense and high-aspect-ratio features, which presents unique challenges in demolding and bonding steps. These fabrication improvements were deployed to produce a microfiltration device that contained closely spaced and high-aspect-ratio columns, suitable for retaining and concentrating cells or beads from a highly diluted suspension.
authors.affiliation: Department of Chemistry, University of Washington, Seattle, WA 98195-1700, USA.
authors.forename: Jason S
authors.initials: JS
authors.lastname: Kuo
authors.forename: Yongxi
authors.initials: Y
authors.lastname: Zhao
authors.forename: Laiying
authors.initials: L
authors.lastname: Ng
authors.forename: Gloria S
authors.initials: GS
authors.lastname: Yen
authors.forename: Robert M
authors.initials: RM
authors.lastname: Lorenz
authors.forename: David S W
authors.initials: DS
authors.lastname: Lim
authors.forename: Daniel T
authors.initials: DT
authors.lastname: Chiu
mesh_terms: D001943:Breast Neoplasms / Q000175:diagnosis; D002458:Cell Fractionation / Q000295:instrumentation; D045744:Cell Line, Tumor; D004867:Equipment Design; D005260:Female; D006801:Humans; D008689:Methacrylates / Q000737:chemistry*; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D008851:Micropore Filters; D011140:Polyurethanes / Q000737:chemistry*
chemical_list: D008689:Methacrylates; D011140:Polyurethanes