title: Cl- and F- anions regulate the architecture of protofibrils in fibrin gel.
abstract: Ischemic heart disease is the leading cause of serious morbidity and mortality in Western society. One of the therapeutic approaches is based on the use of thrombolitic drugs that promote clot lysis. Even if the mechanisms leading to clot lysis are not completely understood, it is widely accepted that they depend on the complex biochemical reactions that occur among fibrin fibers and fibrinolitic agents, and by their ready diffusion into the fibers. Here we investigate the effects of specific anions on the architecture of protofibrils within fibrin fibers in fibrin gels prepared in a para-physiological solution. The results obtained through small-angle X-ray scattering (SAXS) demonstrate that the characteristic axial and longitudinal repeat distances among protofibrils are strongly affected by the action of Cl(-) and F(-) anions.
authors.affiliation: Istituto di Fisica, Università Cattolica del Sacro Cuore, Largo F. Vito 1, Rome, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Missori
authors.forename: M
authors.initials: M
authors.lastname: Papi
authors.forename: G
authors.initials: G
authors.lastname: Maulucci
authors.forename: G
authors.initials: G
authors.lastname: Arcovito
authors.forename: G
authors.initials: G
authors.lastname: Boumis
authors.forename: A
authors.initials: A
authors.lastname: Bellelli
authors.forename: G
authors.initials: G
authors.lastname: Amiconi
authors.forename: M
authors.initials: M
authors.lastname: De Spirito
mesh_terms: D000838:Anions / Q000737:chemistry; D002712:Chlorides / Q000737:chemistry*; D005337:Fibrin / Q000737:chemistry*; D005343:Fibrinolytic Agents / Q000378:metabolism; D005459:Fluorides / Q000737:chemistry*; D005782:Gels / Q000737:chemistry*; D011487:Protein Conformation; D012542:Scattering, Radiation; D053838:Scattering, Small Angle; D014961:X-Ray Diffraction / Q000379:methods; D014965:X-Rays
chemical_list: D000838:Anions; D002712:Chlorides; D005343:Fibrinolytic Agents; D005782:Gels; D005337:Fibrin; D005459:Fluorides