title: Association of polymorphisms -1154G/A and -2578C/A in the vascular endothelial growth factor gene with decreased risk of endometriosis in Chinese women.
abstract: BACKGROUND
  Vascular endothelial growth factor (VEGF) plays an important role in the development of endometriosis. The aim of this study was to investigate the association of polymorphisms in the VEGF gene with the susceptibility to endometriosis.
  
  
  METHODS
  This study comprised 344 North Chinese women with endometriosis and 360 healthy women without endometriosis recruited as control. Genotyping of the VEGF gene polymorphisms at -460C/T, -1154G/A, -2578C/A and +936C/T were performed by PCR and restriction fragment length polymorphism analysis.
  
  
  RESULTS
  No significant difference was found in allele and genotype distributions of the -460C/T, +936C/T polymorphisms between patients and controls. However, the frequencies of -1154G/A, -2578C/A genotype and allele were significantly different between the two groups (all P-value <0.013). The -2578A/A, -1154A/A genotypes were found less frequently in patients with endometriosis compared with controls. The haplotype distributions derived from three polymorphisms (-2578C/A, -1154G/A, -460C/T) differed between the two groups (P = 0.000).
  
  
  CONCLUSIONS
  The VEGF-460/-1154/-2578 TGC, CAA, TAA and TAC haplotypes were associated with endometriosis. The -1154A and -2578A alleles may be protective against the development of endometriosis in North Chinese women.
authors.affiliation: Department of Obstetrics and Gynaecology, Fourth Affiliated Hospital, Hebei Medical University, Jiankanglu 12, Shijiazhuang, China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Liu
authors.forename: Yan
authors.initials: Y
authors.lastname: Li
authors.forename: Jian
authors.initials: J
authors.lastname: Zhao
authors.forename: Dong-Lan
authors.initials: DL
authors.lastname: Sun
authors.forename: Ya-Nan
authors.initials: YN
authors.lastname: Duan
authors.forename: Na
authors.initials: N
authors.lastname: Wang
authors.forename: Rong-Miao
authors.initials: RM
authors.lastname: Zhou
authors.forename: Shan
authors.initials: S
authors.lastname: Kang
mesh_terms: D000328:Adult; D016022:Case-Control Studies; D002681:China; D004715:Endometriosis / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D006239:Haplotypes; D006801:Humans; D011110:Polymorphism, Genetic; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics*
chemical_list: C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A