title: Structural study and conformational behavior of the two different lipopolysaccharide O-antigens produced by the cystic fibrosis pathogen Burkholderia multivorans.
abstract: Lipopolysaccharides (LPSs) are virulence factors expressed by gram-negative bacteria; they are among those mainly responsible for bacterial virulence. In this work we define the primary structure and the conformational features of the O-chain from the LPS produced by the highly virulent clinical isolate Burkholderia multivorans strain C1576, an opportunistic human pathogen isolated in a cystic fibrosis center and causative of an outbreak with lethal outcome. We demonstrate that the LPS from this clinical isolate consists of two O-polysaccharide chains present in different amounts and made up of repeating units, both containing deoxy sugar. Additionally, conformational studies have been performed to establish and compare the spatial arrangements of the two polysaccharides and differences in their shape have been highlighted. The comprehension of the structural and conformational features of the two repeating units may help to explain their biological significance, the molecular shape of the bacterial external surface, and the comprehension at the molecular level of the recognition mechanisms of the antibodies.
authors.affiliation: Dipartimento di Chimica Organica e Biochimica, Università di Napoli Federico II, Complesso Universitario Monte S. Angelo, Via Cintia 4, 80126 Napoli, Italy.
authors.forename: Teresa
authors.initials: T
authors.lastname: Ieranò
authors.forename: Alba
authors.initials: A
authors.lastname: Silipo
authors.forename: Paola
authors.initials: P
authors.lastname: Cescutti
authors.forename: Maria Rosaria
authors.initials: MR
authors.lastname: Leone
authors.forename: Roberto
authors.initials: R
authors.lastname: Rizzo
authors.forename: Rosa
authors.initials: R
authors.lastname: Lanzetta
authors.forename: Michelangelo
authors.initials: M
authors.lastname: Parrilli
authors.forename: Antonio
authors.initials: A
authors.lastname: Molinaro
mesh_terms: D019117:Burkholderia / Q000276:immunology*; D019121:Burkholderia Infections / Q000276:immunology / Q000382:microbiology*; D002236:Carbohydrate Conformation; D003550:Cystic Fibrosis / Q000276:immunology* / Q000382:microbiology; D006801:Humans; D008070:Lipopolysaccharides / Q000737:chemistry / Q000276:immunology*; D009682:Magnetic Resonance Spectroscopy; D008958:Models, Molecular; D008969:Molecular Sequence Data; D019081:O Antigens / Q000737:chemistry / Q000276:immunology*; D037521:Virulence Factors
chemical_list: D008070:Lipopolysaccharides; D019081:O Antigens; D037521:Virulence Factors