title: Paramagnetic labelling of proteins and oligonucleotides for NMR.
abstract: Paramagnetic effects offer a rich source of long-range structural restraints. Here we review current methods for site-specific tagging of proteins and oligonucleotides with paramagnetic molecules. The paramagnetic tags include nitroxide radicals and metal chelators. Particular emphasis is placed on tags suitable for site-specific and rigid attachment of lanthanide ions to macromolecules.
authors.affiliation: The Australian National University, Canberra, Australia.
authors.forename: Xun-Cheng
authors.initials: XC
authors.lastname: Su
authors.forename: Gottfried
authors.initials: G
authors.lastname: Otting
mesh_terms: D004578:Electron Spin Resonance Spectroscopy / Q000379:methods*; D028581:Lanthanoid Series Elements / Q000737:chemistry; D008958:Models, Molecular; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods*; D009841:Oligonucleotides / Q000737:chemistry*; D011487:Protein Conformation; D011506:Proteins / Q000737:chemistry*; D013113:Spin Labels
chemical_list: D028581:Lanthanoid Series Elements; D009841:Oligonucleotides; D011506:Proteins; D013113:Spin Labels