title: Quantifying methane oxidation in a landfill-cover soil by gas push-pull tests.
abstract: Methane (CH(4)) oxidation by aerobic methanotrophs in landfill-cover soils decreases emissions of landfill-produced CH(4) to the atmosphere. To quantify in situ rates of CH(4) oxidation we performed five gas push-pull tests (GPPTs) at each of two locations in the cover soil of the Lindenstock landfill (Liestal, Switzerland) over a 4 week period. GPPTs consist of the injection of a gas mixture containing CH(4), O(2) and noble gas tracers followed by extraction from the same location. Quantification of first-order rate constants was based upon comparison of breakthrough curves of CH(4) with either Ar or CH(4) itself from a subsequent inactive GPPT containing acetylene as an inhibitor of CH(4) oxidation. The maximum calculated first-order rate constant was 24.8+/-0.8 h(-1) at location 1 and 18.9+/-0.6 h(-1) at location 2. In general, location 2 had higher background CH(4) concentrations in vertical profile samples than location 1. High background CH(4) concentrations in the cover soil during some experiments adversely affected GPPT breakthrough curves and data interpretation. Real-time PCR verified the presence of a large population of methanotrophs at the two GPPT locations and comparison of stable carbon isotope fractionation of CH(4) in an active GPPT and a subsequent inactive GPPT confirmed that microbial activity was responsible for the CH(4) oxidation. The GPPT was shown to be a useful tool to reproducibly estimate in situ rates of CH(4) oxidation in a landfill-cover soil when background CH(4) concentrations were low.
authors.affiliation: Institute of Biogeochemistry and Pollutant Dynamics, ETH Zürich, Universitätstrasse 16, 8092 Zürich, Switzerland. gomezke@hotmail.com
authors.forename: K E
authors.initials: KE
authors.lastname: Gómez
authors.forename: G
authors.initials: G
authors.lastname: Gonzalez-Gil
authors.forename: A
authors.initials: A
authors.lastname: Lazzaro
authors.forename: M H
authors.initials: MH
authors.lastname: Schroth
mesh_terms: D002247:Carbon Isotopes / Q000032:analysis; D005591:Chemical Fractionation; D007700:Kinetics; D008697:Methane / Q000032:analysis / Q000378:metabolism*; D008772:Methylococcaceae / Q000378:metabolism*; D010084:Oxidation-Reduction; D016133:Polymerase Chain Reaction; D012037:Refuse Disposal; D012987:Soil / Q000032:analysis*; D012988:Soil Microbiology
chemical_list: D002247:Carbon Isotopes; D012987:Soil; D008697:Methane