Combinatorial chemistry on solid support in the search for central nervous system agents.
The advent of combinatorial chemistry was one of the most important developments, that has significantly contributed to the drug discovery process. Within just a few years, its initial concept aimed at production of libraries containing huge number of compounds (thousands to millions), so called screening libraries, has shifted towards preparation of small and medium-sized rationally designed libraries. When applicable, the use of solid supports for the generation of libraries has been a real breakthrough in enhancing productivity. With a limited amount of resin and simple manual workups, the split/mix procedure may generate thousands of bead-tethered compounds. Beads can be chemically or physically encoded to facilitate the identification of a hit after the biological assay. Compartmentalization of solid supports using small reactors like teabags, kans or pellicular discrete supports like Lanterns resulted in powerful sort and combine technologies, relying on codes 'written' on the reactor, and thus reducing the need for automation and improving the number of compounds synthesized. These methods of solid-phase combinatorial chemistry have been recently supported by introduction of solid-supported reagents and scavenger resins. The first part of this review discusses the general premises of combinatorial chemistry and some methods used in the design of primary and focused combinatorial libraries. The aim of the second part is to present combinatorial chemistry methodologies aimed at discovering bioactive compounds acting on diverse GPCR involved in central nervous system disorders.
Zajdel
Paweł
P
Department of Medicinal Chemistry, Jagiellonian University Medical College, 30-688 Kraków, Poland. mfzajdel@cyf-kr.edu.pl
Pawłowski
Maciej
M
Martinez
Jean
J
Subra
Gilles
G
D000818:Animals; D001569:Benzodiazepines / Q000737:chemistry; D002491:Central Nervous System Agents / Q000138:chemical synthesis*; D020650:Combinatorial Chemistry Techniques / Q000379:methods*; D015195:Drug Design; D006801:Humans; D015394:Molecular Structure
D002491:Central Nervous System Agents; D001569:Benzodiazepines