title: Identification and characterization of a novel Tritimovirus species isolated from wild Trisetum flavescens L., family Poaceae.
abstract: Yellow oat-grass plants (Trisetum flavescens L.) with mild mosaic and pronounced dwarfing symptoms were observed at different locations in the Czech Republic. Electron microscope observations of symptomatic plants revealed the presence of filamentous particles and inclusion bodies characteristic of the family Potyviridae. The virus was readily mechanically transmitted to its original host plus a narrow host range of monocot species. Serological assays of infected plant extracts using antiserum specific to the closest species in the family Potyviridae were negative. The 3' end of the viral genome was cloned, sequenced and compared to sequences of species in the family Potyviridae. The virus is more closely related to viruses in the genus Tritimovirus than to other genera within the Potyviridae. Based on phylogenetic analyses of the coat protein cistron and flanking genomic regions, we propose this is a distinct viral species of the genus Tritimovirus, tentatively named Yellow oat-grass mosaic virus (YOgMV).
authors.affiliation: Agricultural Botany Department (Plant Pathology Sector), Fayoum University, Fayoum, Egypt. Moha_hassan@yahoo.com
authors.forename: Mohamed
authors.initials: M
authors.lastname: Hassan
authors.forename: Lenka
authors.initials: L
authors.lastname: Sirlova
authors.forename: Milan
authors.initials: M
authors.lastname: Jokes
authors.forename: Josef
authors.initials: J
authors.lastname: Vacke
mesh_terms: D000595:Amino Acid Sequence; D036022:Capsid Proteins / Q000235:genetics; D016000:Cluster Analysis; D018153:Czech Republic; D002479:Inclusion Bodies / Q000821:virology; D008969:Molecular Sequence Data; D010802:Phylogeny; D010935:Plant Diseases / Q000821:virology*; D006109:Poaceae / Q000821:virology*; D019176:Potyviridae / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D012367:RNA, Viral / Q000235:genetics; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D014771:Virion / Q000648:ultrastructure
chemical_list: D036022:Capsid Proteins; D012367:RNA, Viral