title: Antimicrobial ent-pimarane diterpenes from Viguiera arenaria against Gram-positive bacteria.
abstract: The dichloromethane crude extract from the roots of Viguiera arenaria (VaDRE) has been employed in an antimicrobial screening against several bacteria responsible for human pathologies. The main diterpenes isolated from this extract, as well as two semi-synthetic pimarane derivatives, were also investigated for the pathogens that were significantly inhibited by the extract (MIC values lower than 100 microg mL(-1)). The VaDRE extract was significantly active only against Gram-positive microorganisms. The compounds ent-pimara-8(14),15-dien-19-oic acid (PA); PA sodium salt; ent-8(14),15-pimaradien-3beta-ol; ent-15-pimarene-8 beta,19-diol; and ent-8(14),15-pimaradien-3beta-acetoxy displayed the highest antibacterial activities (MIC values lower than 10 microg mL(-1) for most pathogens). In conclusion, our results suggest that pimaranes are an important class of natural products for further investigations in the search of new antibacterial agents.
authors.affiliation: Núcleo de Pesquisas em Ciências Exatas e Tecnológicas, Universidade de Franca, Franca, SP, Brazil.
authors.forename: Thiago Souza
authors.initials: TS
authors.lastname: Porto
authors.forename: Niege A J C
authors.initials: NA
authors.lastname: Furtado
authors.forename: Vladimir C G
authors.initials: VC
authors.lastname: Heleno
authors.forename: Carlos H G
authors.initials: CH
authors.lastname: Martins
authors.forename: Fernando B
authors.initials: FB
authors.lastname: Da Costa
authors.forename: Marcela E
authors.initials: ME
authors.lastname: Severiano
authors.forename: Aline N
authors.initials: AN
authors.lastname: Silva
authors.forename: Rodrigo C S
authors.initials: RC
authors.lastname: Veneziani
authors.forename: Sérgio R
authors.initials: SR
authors.lastname: Ambrósio
mesh_terms: D045784:Abietanes / Q000302:isolation & purification / Q000494:pharmacology*; D000900:Anti-Bacterial Agents / Q000302:isolation & purification / Q000494:pharmacology*; D019659:Asteraceae / Q000737:chemistry*; D004224:Diterpenes / Q000302:isolation & purification / Q000494:pharmacology*; D006094:Gram-Positive Bacteria / Q000187:drug effects*; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D018517:Plant Roots
chemical_list: D045784:Abietanes; D000900:Anti-Bacterial Agents; D004224:Diterpenes; D010936:Plant Extracts