title: A comparison of the packing behavior of egg phosphatidylcholine with cholesterol and biogenically related sterols in Langmuir monolayer films.
abstract: Cholesterol and selected derivatives were studied as mixed Langmuir monolayers with egg phosphatidylcholine (PC). As an extension of our earlier work, which employed binary sterol/PC mixtures, here we examined ternary mixed monolayers containing cholesterol along with an alternate sterol and PC in different molar ratios, using pressure-area isotherms. The ternary systems behaved similarly to the binary sterol/PC systems reported previously, with similar condensation noted for the sterol/PC films. To better understand how variations in sterol structure affect sterol packing in such membrane monolayers, binary mixtures containing cholestenone, cholestanol, and lanosterol with PC were also studied. Cholestanol behaved similarly to cholesterol when incorporated with PC, while cholestenone and lanosterol did not cause as much film condensation. The observed differences in molecular packing, and attributed sterol structural differences, are considered within the context of sterol/phospholipid mixtures in biological membranes.
authors.affiliation: Department of Chemistry, Saint Louis University, St. Louis, MO 63103-2010, USA.
authors.forename: Kimberly Borrenpohl
authors.initials: KB
authors.lastname: Lintker
authors.forename: Peter
authors.initials: P
authors.lastname: Kpere-Daibo
authors.forename: Steven J
authors.initials: SJ
authors.lastname: Fliesler
authors.forename: Alexa Barnoski
authors.initials: AB
authors.lastname: Serfis
mesh_terms: D000818:Animals; D004083:Cholestanol / Q000737:chemistry*; D008563:Membrane Lipids / Q000737:chemistry*; D008958:Models, Molecular; D015394:Molecular Structure; D010063:Ovum / Q000737:chemistry; D010713:Phosphatidylcholines / Q000737:chemistry*; D011312:Pressure; D013261:Sterols / Q000737:chemistry*; D013696:Temperature
chemical_list: D008563:Membrane Lipids; D010713:Phosphatidylcholines; D013261:Sterols; D004083:Cholestanol