[Modification of recombinant proteins by covalent polysialation illustrated with the example of human insulin].
Methods of selective and nonselective covalent immobilization of genetically engineered proteins on molecules of natural polysialic acid are described by the example of human insulin. Such modification increases insulin lifetime in vivo.
Bezuglov
V V
VV
Gretskaia
N M
NM
Bobrov
M Iu
MIu
Akimov
M G
MG
Fomina-Ageeva
E V
EV
Zinchenko
G N
GN
Bairamashvili
D I
DI
Miroshnikov
A I
AI
D006801:Humans; D007328:Insulin / Q000737:chemistry*; D011994:Recombinant Proteins / Q000737:chemistry; D012794:Sialic Acids / Q000737:chemistry*
D007328:Insulin; D011994:Recombinant Proteins; D012794:Sialic Acids; C021319:polysialic acid