title: Photochemical regulation of restriction endonuclease activity.
abstract: Removal by the light: The photochemical regulation of restriction endonucleases, which are important enzymes in molecular biology, has been investigated. Photolabile protecting groups have been installed on DNA substrates and have been demonstrated to inhibit restriction endonuclease activity until removed by UV light irradiation. Interestingly, these groups do not appear to dramatically affect initial binding of the enzyme to the DNA substrate, but rather prevent recognition of the specific cleavage site.
authors.affiliation: Department of Chemistry, North Carolina State University, Raleigh, NC 27695, USA.
authors.forename: Douglas D
authors.initials: DD
authors.lastname: Young
authors.forename: Jeane M
authors.initials: JM
authors.lastname: Govan
authors.forename: Mark O
authors.initials: MO
authors.lastname: Lively
authors.forename: Alexander
authors.initials: A
authors.lastname: Deiters
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D001483:Base Sequence; D052117:Benzodioxoles / Q000737:chemistry*; D004247:DNA / Q000737:chemistry* / Q000528:radiation effects; D053837:DNA Cleavage; D004262:DNA Restriction Enzymes / Q000235:genetics / Q000378:metabolism*; D015245:Deoxyribonuclease BamHI / Q000235:genetics / Q000378:metabolism; D015246:Deoxyribonuclease EcoRI / Q000235:genetics / Q000378:metabolism; D015252:Deoxyribonucleases, Type II Site-Specific / Q000235:genetics / Q000378:metabolism; D013379:Substrate Specificity; D013936:Thymidine / Q000031:analogs & derivatives* / Q000737:chemistry; D014466:Ultraviolet Rays
chemical_list: D001426:Bacterial Proteins; D052117:Benzodioxoles; D004247:DNA; D004262:DNA Restriction Enzymes; D015245:Deoxyribonuclease BamHI; D015246:Deoxyribonuclease EcoRI; C024404:BglII endonuclease; D015252:Deoxyribonucleases, Type II Site-Specific; D013936:Thymidine