Experimental challenge of bovine mammary glands with Enterococcus faecium during early and late lactation.
Mammary glands of early and late lactation cows were challenged with Enterococcus faecium of bovine origin to determine in vivo pathogenicity and milk somatic cell count (SCC) responses. A total of 20 early lactation and 18 late lactation mammary glands were challenged. Two isolates highly adaptive and 2 isolates poorly adaptive for in vitro growth in mammary secretion were used as challenge strains of bacteria. Challenged quarters of early lactation cows were more susceptible to intramammary infection caused by E. faecium than those of late lactation cows. Intramammary challenge with isolates poorly adaptive for in vitro growth in mammary secretions resulted in 94.7% of quarters infected compared with 36.8% of the quarters infused with the isolates highly adaptive for in vitro growth in mammary secretions. Milk from quarters infused with the isolates poorly adaptive for in vitro growth had higher SCC and bacterial counts compared with quarters challenged with the isolates highly adaptive for in vitro growth. A stage of lactation effect within treatment groups was measured when milk SCC were compared between early and late lactation cows. Milk SCC in uninfused (negative control) quarters were lower in early lactation cows compared with late lactation cows. Conversely, in quarters infused with isolates poorly adaptive for in vitro growth, SCC were higher in early lactation cows compared with late lactation cows on d 2, 3, 4, 15, 16, and 17 postchallenge. In quarters infused with isolates highly adaptive for in vitro growth, SCC response did not differ between early and late lactation cows. In vitro growth of E. faecium in mammary secretion was inversely related to in vivo pathogenicity in the mammary glands of early and late lactation cows.
Petersson-Wolfe
C S
CS
Department of Animal Sciences, The Ohio State University, Ohio Agricultural Research and Development Center, Wooster 44691, USA.
Wolf
S L
SL
Hogan
J S
JS
D000818:Animals; D002417:Cattle; D002452:Cell Count; D015169:Colony Count, Microbial; D016984:Enterococcus faecium / Q000472:pathogenicity* / Q000502:physiology; D005260:Female; D016908:Gram-Positive Bacterial Infections / Q000662:veterinary*; D007774:Lactation / Q000502:physiology*; D008321:Mammary Glands, Animal / Q000382:microbiology*; D008414:Mastitis, Bovine / Q000382:microbiology* / Q000503:physiopathology; D008892:Milk / Q000166:cytology / Q000382:microbiology