Structural study and conformational behavior of the two different lipopolysaccharide O-antigens produced by the cystic fibrosis pathogen Burkholderia multivorans.
Lipopolysaccharides (LPSs) are virulence factors expressed by gram-negative bacteria; they are among those mainly responsible for bacterial virulence. In this work we define the primary structure and the conformational features of the O-chain from the LPS produced by the highly virulent clinical isolate Burkholderia multivorans strain C1576, an opportunistic human pathogen isolated in a cystic fibrosis center and causative of an outbreak with lethal outcome. We demonstrate that the LPS from this clinical isolate consists of two O-polysaccharide chains present in different amounts and made up of repeating units, both containing deoxy sugar. Additionally, conformational studies have been performed to establish and compare the spatial arrangements of the two polysaccharides and differences in their shape have been highlighted. The comprehension of the structural and conformational features of the two repeating units may help to explain their biological significance, the molecular shape of the bacterial external surface, and the comprehension at the molecular level of the recognition mechanisms of the antibodies.
Ieranò
Teresa
T
Dipartimento di Chimica Organica e Biochimica, Università di Napoli Federico II, Complesso Universitario Monte S. Angelo, Via Cintia 4, 80126 Napoli, Italy.
Silipo
Alba
A
Cescutti
Paola
P
Leone
Maria Rosaria
MR
Rizzo
Roberto
R
Lanzetta
Rosa
R
Parrilli
Michelangelo
M
Molinaro
Antonio
A
D019117:Burkholderia / Q000276:immunology*; D019121:Burkholderia Infections / Q000276:immunology / Q000382:microbiology*; D002236:Carbohydrate Conformation; D003550:Cystic Fibrosis / Q000276:immunology* / Q000382:microbiology; D006801:Humans; D008070:Lipopolysaccharides / Q000737:chemistry / Q000276:immunology*; D009682:Magnetic Resonance Spectroscopy; D008958:Models, Molecular; D008969:Molecular Sequence Data; D019081:O Antigens / Q000737:chemistry / Q000276:immunology*; D037521:Virulence Factors
D008070:Lipopolysaccharides; D019081:O Antigens; D037521:Virulence Factors