Expression of bone morphogenetic proteins in giant cell tumor of bone.
BACKGROUND
A giant cell tumor (GCT) of bone is a locally aggressive tumor with a propensity for local recurrence. A characteristic pattern of peripheral bone formation has been described in GCT recurrence in soft tissue, and in some pulmonary metastases from benign GCT. Although the bone formation in GCT in supposedly due to bone morphogenetic proteins (BMPs), the expression pattern of BMPs in GCT has not been well investigated.


METHODS
The expression of BMPs in GCT tissues, cultured stromal cells from GCT, and osteoclast-like giant cells harvested by laser microdissection (LM), as well as from control osteosarcoma (NOS-1) cells was analyzed using reverse transcriptional-semiquantitative PCR.


RESULTS
BMP 2, 3, 4, 5 and 6 were expressed in the GCT tissue. The cultured GCT cells expressed BMP 2, 4, 5 and 6. The osteoclast-like giant cells expressed BMP 2, 3, 5 and 6 and BMP 5 was expressed at the highest level.


CONCLUSIONS
Both stromal cells and osteoclast-like cells in GCT expressed several kinds of BMPs.
Kudo
Naoko
N
Division of Orthopedic Surgery, Department of Regenerative Transplant Medicine, Niigata University Graduate School of Medical and Dental Sciences, Niigata City, Niigata 951-8510, Japan.
Ogose
Akira
A
Ariizumi
Takashi
T
Kawashima
Hiroyuki
H
Hotta
Tetsuo
T
Hatano
Hiroshi
H
Morita
Tetsuro
T
Nagata
Masaki
M
Siki
Yukie
Y
Kawai
Akira
A
Hotta
Yuko
Y
Hoshino
Makiko
M
Endo
Naoto
N
D000328:Adult; D019485:Bone Morphogenetic Proteins / Q000235:genetics* / Q000378:metabolism; D001859:Bone Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D005260:Female; D018212:Giant Cell Tumor of Bone / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D006801:Humans; D007834:Lasers; D008297:Male; D042282:Microdissection; D008875:Middle Aged; D012516:Osteosarcoma / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017154:Stromal Cells / Q000378:metabolism / Q000473:pathology; D014407:Tumor Cells, Cultured; D055815:Young Adult
D019485:Bone Morphogenetic Proteins; D012333:RNA, Messenger