title: Direct electron transfer of glucose oxidase immobilized in an ionic liquid reconstituted cellulose-carbon nanotube matrix.
abstract: Conductive cellulose-multiwalled carbon nanotube (MWCNT) matrix with a porous structure and good biocompatibility has been prepared using a room temperature ionic liquid (1-ethyl-3-methylimidazolium acetate) as solvent. Glucose oxidase (GOx) was encapsulated in this matrix and thereby immobilized on a glassy carbon surface. The direct electron transfer and electrocatalysis of the encapsulated GOx has been investigated using cyclic voltammetry and chronoamperometry. The GOx exhibited a pair of stable, well defined and nearly symmetric reversible redox peaks. The experimental results also demonstrate that the immobilized GOx retains its biocatalytic activity toward the oxidation of glucose and therefore can be employed in a glucose biosensor. The results show that the bioelectrode modified by the cellulose-MWCNT matrix has potential for use in biosensors and other bioelectronics devices.
authors.affiliation: Chemical Sciences, University of Surrey, Guildford, GU2 7XH, United Kingdom. Xuee.wu@surrey.ac.uk
authors.forename: Xuee
authors.initials: X
authors.lastname: Wu
authors.forename: Feng
authors.initials: F
authors.lastname: Zhao
authors.forename: John R
authors.initials: JR
authors.lastname: Varcoe
authors.forename: Alfred E
authors.initials: AE
authors.lastname: Thumser
authors.forename: Claudio
authors.initials: C
authors.lastname: Avignone-Rossa
authors.forename: Robert C T
authors.initials: RC
authors.lastname: Slade
mesh_terms: D001234:Aspergillus niger / Q000201:enzymology; D055162:Biocatalysis; D015374:Biosensing Techniques; D020134:Catalytic Domain; D002482:Cellulose / Q000737:chemistry*; D004563:Electrochemistry; D004579:Electron Transport; D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000737:chemistry* / Q000378:metabolism*; D005949:Glucose Oxidase / Q000737:chemistry* / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007093:Imidazoles / Q000737:chemistry; D052578:Ionic Liquids / Q000737:chemistry*; D037742:Nanotubes, Carbon / Q000737:chemistry*; D015203:Reproducibility of Results; D012996:Solutions; D012997:Solvents / Q000737:chemistry
chemical_list: D004800:Enzymes, Immobilized; D007093:Imidazoles; D052578:Ionic Liquids; D037742:Nanotubes, Carbon; D012996:Solutions; D012997:Solvents; C502841:1-butyl-3-methylimidazolium chloride; D002482:Cellulose; D005949:Glucose Oxidase; C518739:1-ethyl-3-methylimidazolium