Baicalin suppresses expression of Chlamydia protease-like activity factor in Hep-2 cells infected by Chlamydia trachomatis.
In this study, we tested the ability of Baicalin to block Chlamydia trachomatis infection and found that the Baicalin blocked infection of Hep-2 cells. Then, we looked into the expression of RFX5 and CPAF gene in Chlamydia-infected cells. We found that RFX5 and CPAF were up-regulated and down-regulated respectively by Baicalin. Since CPAF is responsible for degrading RFX5, we suggest that CPAF was a primary target of Baicalin and played an important role in regulating RFX5. Our findings demonstrate that Baicalin can inhibit C. trachomatis effectively and therefore, can be considered as potential agents for therapy of Chlamydia infectious diseases.
Hao
Huang
H
Center of Experimental Medicine, Wuhan No.1 Hospital, Wuhan 430022, PR China. whhuanghao10@yahoo.com.cn
Aixia
Yang
Y
Dan
Li
L
Lei
Fu
F
Nancai
Yu
Y
Wen
Su
S
D000900:Anti-Bacterial Agents / Q000494:pharmacology* / Q000627:therapeutic use; D002460:Cell Line; D002690:Chlamydia Infections / Q000188:drug therapy*; D002692:Chlamydia trachomatis / Q000187:drug effects* / Q000235:genetics / Q000472:pathogenicity; D005419:Flavonoids / Q000494:pharmacology* / Q000627:therapeutic use; D015870:Gene Expression / Q000187:drug effects*; D005786:Gene Expression Regulation / Q000187:drug effects; D005798:Genes, Bacterial; D006801:Humans; D008517:Phytotherapy; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use; D012333:RNA, Messenger / Q000378:metabolism; D031364:Scutellaria baicalensis / Q000737:chemistry
D000900:Anti-Bacterial Agents; D005419:Flavonoids; D010936:Plant Extracts; D012333:RNA, Messenger; C499814:Scutellaria baicalensis extract; C038044:baicalin