Dexamethasone regulates expression of BRUCE/Apollon and the proliferation of neural progenitor cells.
Glucocorticoid hormones (GHs) regulate cell proliferation of neural progenitor cells (NPCs) contributing to reduction of neurogenesis after stress. We show here that dexamethasone (Dex) decreases BRUCE/Apollon (BRUCE) in cultured NPCs in a GH-receptor-dependent manner. Downregulation of BRUCE by Dex or using silencing RNA reduced the number of proliferating NPCs, whilst overexpression of BRUCE counteracted the effect of Dex. Dex also elevated the deubiquitinating enzyme, Usp8/Ubpy, which via Nrdp1 decreases BRUCE. The results show that BRUCE is a target for GHs in the NPCs, and that BRUCE controls cell division of NPCs and possibly of other stem cells.
Sippel
Maria
M
Minerva Medical Research Institute, Biomedicum, Helsinki, Finland.
Rajala
Raili
R
Korhonen
Laura
L
Bornhauser
Beat
B
Sokka
Anna-Leena
AL
Naito
Mikihiko
M
Lindholm
Dan
D
D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects*; D003907:Dexamethasone / Q000494:pharmacology*; D015536:Down-Regulation; D004622:Embryo, Mammalian / Q000378:metabolism; D005786:Gene Expression Regulation; D055495:Neurogenesis; D009474:Neurons / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D051381:Rats; D013234:Stem Cells / Q000166:cytology / Q000187:drug effects*; D044763:Ubiquitin-Conjugating Enzymes / Q000235:genetics* / Q000378:metabolism; D064570:Ubiquitin-Specific Proteases / Q000235:genetics / Q000378:metabolism
D003907:Dexamethasone; D044763:Ubiquitin-Conjugating Enzymes; C000598578:USP8 protein, rat; D064570:Ubiquitin-Specific Proteases; C506881:Birc6 protein, rat