title: Comparative analysis of the production of nitric oxide (NO) and tumor necrosis factor-alpha (TNF-alpha) from macrophages exposed to high virulent and low virulent strains of Edwardsiella tarda.
abstract: We previously reported that high virulent strain (NUF251) of Edwardsiella tarda has an ability to prevent the production of reactive oxygen species by macrophages, and is even capable of surviving and multiplying within Japanese flounder (Paralichthys olivaceus) peritoneal macrophages, whereas the low virulent strain (NUF194) has no such ability. In this study, we found that NUF251 and NUF194 induced NO and TNF-alpha production from Japanese flounder peritoneal macrophages, and NUF251 caused faster induction of NO release and much higher level of TNF-alpha production than NUF194. In addition, similar differences between two strains in terms of the induction of NO and TNF-alpha production were also observed in mouse macrophage cell line RAW264.7 cells. Our results suggest that the potent ability to induce the production of NO and TNF-alpha from macrophages may be one of the factors responsible for the virulence of E. tarda.
authors.affiliation: Graduate School of Science and Technology, Nagasaki University, Nagasaki 852-8521, Japan.
authors.forename: Keiko
authors.initials: K
authors.lastname: Ishibe
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Yamanishi
authors.forename: Yajun
authors.initials: Y
authors.lastname: Wang
authors.forename: Kiyoshi
authors.initials: K
authors.lastname: Osatomi
authors.forename: Kenji
authors.initials: K
authors.lastname: Hara
authors.forename: Kinya
authors.initials: K
authors.lastname: Kanai
authors.forename: Kenichi
authors.initials: K
authors.lastname: Yamaguchi
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Oda
mesh_terms: D000818:Animals; D002460:Cell Line; D020609:Edwardsiella tarda / Q000472:pathogenicity*; D004756:Enterobacteriaceae Infections / Q000276:immunology*; D005393:Fish Diseases / Q000276:immunology; D005432:Flounder / Q000276:immunology*; D017737:Macrophages, Peritoneal / Q000276:immunology*; D051379:Mice; D009569:Nitric Oxide / Q000276:immunology*; D013997:Time Factors; D014409:Tumor Necrosis Factor-alpha / Q000276:immunology*
chemical_list: D014409:Tumor Necrosis Factor-alpha; D009569:Nitric Oxide