title: A recombinant-based ELISA evaluating the efficacy of netobimin and albendazole in ruminants with naturally acquired fascioliasis.
abstract: The therapeutic efficacy of albendazole and netobimin in ruminants with naturally occurring fascioliasis was investigated using a recombinant-based ELISA. The variation in the IgG response against a 2.9-kDa recombinant protein (FhrAPS), termed efficacy index (EI) 1, and the egg-output changes, termed EI 2, were used to evaluate drug efficacy. The values of EI 1 ranged between 0% and 50% in sheep, and between 0% and 30% in cattle after treatment with albendazole and netobimin. Similar EI 2 values were observed in sheep receiving albendazole or netobimin, but the highest values were found in cattle treated with netobimin. The significant reduction in the IgG response to FhrAPS found in this study shows promise in terms of developing alternative methods for evaluating the efficacy of chemotherapy against Fasciola hepatica in grazing ruminants.
authors.affiliation: Animal Pathology Department, Epidemiology, Zoonoses and Parasitic Diseases, Veterinary Faculty, Santiago de Compostela University, 27002 Lugo, Spain.
authors.forename: María Sol
authors.initials: MS
authors.lastname: Arias
authors.forename: José Luis
authors.initials: JL
authors.lastname: Suárez
authors.forename: G V
authors.initials: GV
authors.lastname: Hillyer
authors.forename: Iván
authors.initials: I
authors.lastname: Francisco
authors.forename: Enrique
authors.initials: E
authors.lastname: Calvo
authors.forename: Rita
authors.initials: R
authors.lastname: Sánchez-Andrade
authors.forename: Pablo
authors.initials: P
authors.lastname: Díaz
authors.forename: Rubén
authors.initials: R
authors.lastname: Francisco
authors.forename: Pablo
authors.initials: P
authors.lastname: Díez-Baños
authors.forename: Patrocinio
authors.initials: P
authors.lastname: Morrondo
authors.forename: Adolfo
authors.initials: A
authors.lastname: Paz-Silva
mesh_terms: D015766:Albendazole / Q000627:therapeutic use*; D000818:Animals; D000871:Anthelmintics / Q000627:therapeutic use*; D000909:Antibodies, Helminth / Q000097:blood / Q000276:immunology; D002417:Cattle; D002418:Cattle Diseases / Q000188:drug therapy*; D004797:Enzyme-Linked Immunosorbent Assay / Q000592:standards / Q000662:veterinary*; D005210:Fasciola hepatica / Q000276:immunology; D005211:Fascioliasis / Q000188:drug therapy / Q000662:veterinary*; D005243:Feces / Q000469:parasitology; D005260:Female; D006146:Guanidines / Q000627:therapeutic use*; D015801:Helminth Proteins / Q000276:immunology; D007074:Immunoglobulin G / Q000097:blood / Q000276:immunology; D010270:Parasite Egg Count / Q000662:veterinary; D011897:Random Allocation; D011994:Recombinant Proteins / Q000276:immunology; D012680:Sensitivity and Specificity; D012756:Sheep; D012757:Sheep Diseases / Q000188:drug therapy*; D016896:Treatment Outcome
chemical_list: D000871:Anthelmintics; D000909:Antibodies, Helminth; D006146:Guanidines; D015801:Helminth Proteins; D007074:Immunoglobulin G; D011994:Recombinant Proteins; D015766:Albendazole; C049626:netobimin