Cell fusion-induced activation of interferon-stimulated genes is not required for restriction of a herpes simplex virus VP16/ICP0 mutant in heterokarya formed between permissive and restrictive cells.
Herpes simplex virus VP16 and ICP0 mutants replicate efficiently in U2OS osteosarcoma cells but are restricted in other cell types. We previously showed that the restrictive phenotype is dominant in a transient cell fusion assay, suggesting that U2OS cells lack an antiviral mechanism present in other cells. Recent data indicate that unscheduled membrane fusion events can activate the expression of interferon-stimulated genes (ISGs) in fibroblasts, raising the possibility that our earlier results were due to a fusion-induced antiviral state. However, we show here that the permissive phenotype is also extinguished following fusion with Vero cells in the absence of ISG induction.
Hancock
Meaghan H
MH
Alberta Institute for Viral Immunology, Department of Medical Microbiology & Immunology, University of Alberta, Edmonton, Alberta T6G 2S2, Canada.
Mossman
Karen L
KL
Smiley
James R
JR
D000818:Animals; D002459:Cell Fusion; D045744:Cell Line, Tumor; D002522:Chlorocebus aethiops; D017405:Herpes Simplex Virus Protein Vmw65 / Q000172:deficiency*; D006801:Humans; D017874:Immediate-Early Proteins / Q000172:deficiency*; D007372:Interferons / Q000096:biosynthesis*; D018139:Simplexvirus / Q000276:immunology* / Q000502:physiology*; D044767:Ubiquitin-Protein Ligases / Q000172:deficiency*; D014709:Vero Cells; D014779:Virus Replication
D017405:Herpes Simplex Virus Protein Vmw65; D017874:Immediate-Early Proteins; D007372:Interferons; D044767:Ubiquitin-Protein Ligases; C051804:Vmw110 protein, Human herpesvirus 1