title: Regulation of interleukin 1alpha, activin and inhibin by lipopolysaccharide in Sertoli cells from prepubertal rats.
abstract: Bacterial lipopolysaccharide increased the production of interleukin 1alpha and activin A, and reduced production of inhibin B, in Sertoli cells from immature male rats measured by enzyme-linked immunosorbent assay (ELISA). The majority of immunoreactive interleukin 1alpha remained within the Sertoli cell, while both activin A and inhibin B were secreted. Lipopolysaccharide-stimulated expression of two interleukin 1alpha mRNA transcripts, measured by quantitative RT-PCR, but the levels of bioactive interleukin 1alpha in Sertoli cell extracts and medium, measured by in vitro bioassay, were comparatively low to undetectable. A specific antagonist of interleukin 1alpha had no effect on lipopolysaccharide-stimulated activin A or inhibin B responses. These data indicate that, in contrast to Sertoli cells from adult rats, lipopolysaccharide-induced regulation of activin A and inhibin B by prepubertal Sertoli cells does not involve secreted interleukin 1alpha. The data highlight the possibility of a role for intracellular interleukin 1alpha in the Sertoli cell response to inflammation, particularly in the immature testis.
authors.affiliation: Monash Institute of Medical Research, Monash University, Monash Medical Centre, Clayton, Melbourne, Victoria, Australia. wendy.winnall@med.monash.edu.au
authors.forename: W R
authors.initials: WR
authors.lastname: Winnall
authors.forename: Y
authors.initials: Y
authors.lastname: Okuma
authors.forename: K
authors.initials: K
authors.lastname: Saito
authors.forename: J A
authors.initials: JA
authors.lastname: Muir
authors.forename: M P
authors.initials: MP
authors.lastname: Hedger
mesh_terms: D028341:Activins / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D001681:Biological Assay; D002457:Cell Extracts; D003470:Culture Media; D005786:Gene Expression Regulation / Q000187:drug effects; D007265:Inhibins / Q000235:genetics / Q000378:metabolism*; D053582:Interleukin-1alpha / Q000235:genetics / Q000378:metabolism*; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008297:Male; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D012708:Sertoli Cells / Q000187:drug effects* / Q000378:metabolism*
chemical_list: D002457:Cell Extracts; D003470:Culture Media; D053582:Interleukin-1alpha; D008070:Lipopolysaccharides; D012333:RNA, Messenger; C422405:activin A; C422364:inhibin B; D028341:Activins; D007265:Inhibins