Expression of semaphorin 5A and its receptor plexin B3 contributes to invasion and metastasis of gastric carcinoma.
OBJECTIVE
To investigate the protein and mRNA expression of semaphorin 5A and its receptor plexin B3 in gastric carcinoma and explore its role in the invasion and metastasis of gastric carcinoma.


METHODS
Expression of semaphorin 5A and its receptor plexin B3 in 48 samples of primary gastric carcinoma, its corresponding non-neoplastic mucosa, and matched regional lymph node metastasis was assayed by reverse transcription-polymerase chain reaction (RT-PCR), real-time RT-PCR and Western blotting.


RESULTS
The protein and mRNA expression of semaphorin 5A and its receptor plexin B3 increased gradually in non-neoplastic mucosa, primary gastric carcinoma and lymph node metastasis (P < 0.05). Moreover, the expression of semaphorin 5A was closely correlated with that of plexin B3.


CONCLUSIONS
Semaphorin 5A and its receptor plexin B3 play an important role in the invasion and metastasis of gastric carcinoma.
Pan
Guo-Qing
GQ
Department of Pathology, Xiangya Medical College, Central South University, Changsha, Hunan Province, China.
Ren
Hong-Zheng
HZ
Zhang
Shu-Fang
SF
Wang
Xi-Mei
XM
Wen
Ji-Fang
JF
D000368:Aged; D000369:Aged, 80 and over; D005260:Female; D005753:Gastric Mucosa / Q000378:metabolism / Q000473:pathology; D006801:Humans; D008207:Lymphatic Metastasis / Q000473:pathology*; D008297:Male; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D008875:Middle Aged; D009361:Neoplasm Invasiveness / Q000473:pathology*; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism*; D019006:Neural Cell Adhesion Molecules / Q000235:genetics / Q000378:metabolism*; D039961:Semaphorins; D013274:Stomach Neoplasms / Q000378:metabolism / Q000473:pathology
D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; D019006:Neural Cell Adhesion Molecules; C496426:PLXNB3 protein, human; C491228:SEMA5A protein, human; D039961:Semaphorins