Rapid detection and quantification of Aspergillus fumigatus in environmental air samples using solid-phase cytometry.
Aspergillus fumigatus is an ubiquitous fungus capable of causing severe infections such as aspergilloma, allergic bronchopulmonary aspergillosis, and invasive aspergillosis, especially in immunocompromised patients. Monitoring the number of Aspergillus fumigatus spores in the air is crucial for infection control. In the present study, a novel approach for the quantification of Aspergillus fumigatus, based on solid-phase cytometry (SPC) and immunofluorescent labeling, was developed. The sensitivity and specificity of the assay were confirmed by testing pure cultures. Paecilomyces variotii and Rhizopus stolonifer were codetected but could be excluded on the basis of morphology of the microcolonies. The SPC method has considerable advantages compared to the culture-based method, including its low detection limit (4 cells/m3), its speed (results are obtained within 24 h), and the straightforward microscopic identification of Aspergillus fumigatus. Additionally, comparison of results obtained with both methods demonstrated that they are equally accurate for the quantification of Aspergillus fumigatus in environmental air samples.
Vanhee
Lies M E
LM
Laboratory of Pharmaceutical Microbiology, Ghent University, Harelbekestraat 72, B-9000 Ghent, Belgium.
Nelis
Hans J
HJ
Coenye
Tom
T
D000391:Air Microbiology; D001232:Aspergillus fumigatus / Q000166:cytology / Q000302:isolation & purification*; D015169:Colony Count, Microbial; D004785:Environmental Pollutants / Q000302:isolation & purification*; D008163:Luminescent Measurements; D008828:Microbiological Techniques / Q000379:methods*; D011142:Polyvinyl Alcohol / Q000737:chemistry; D013172:Spores, Fungal / Q000166:cytology
D004785:Environmental Pollutants; D011142:Polyvinyl Alcohol