title: Differential requirement of ribosomal protein S6 by plant RNA viruses with different translation initiation strategies.
abstract: Potyvirus infection has been reported to cause an increase in the mRNA transcripts of many plant ribosomal proteins (r-proteins). In this study, increased expression of r-protein mRNA transcripts was determined to occur in Nicotiana benthamiana during infection by potyviruses as well as a tobamovirus demonstrating that this response is not unique to potyviruses. Five r-protein genes, RPS6, RPL19, RPL13, RPL7, and RPS2, were silenced in N. benthamiana to test their roles in viral infection. The accumulation of both Turnip mosaic virus (TuMV), a potyvirus, and Tobacco mosaic virus (TMV), a tobamovirus, was dependent on RPL19, RPL13, RPL7, and RPS2. However, TMV was able to accumulate in RPS6-silenced plants while accumulation of TuMV and Tomato bushy stunt virus (TBSV) was abolished. These results demonstrate that cap-independent TuMV and TBSV require RPS6 for their accumulation, whereas accumulation of TMV is independent of RPS6.
authors.affiliation: Department of Plant Pathology, Iowa State University, 351 Bessey Hall, Ames, IA 50011-1020, USA.
authors.forename: Chunling
authors.initials: C
authors.lastname: Yang
authors.forename: Chunquan
authors.initials: C
authors.lastname: Zhang
authors.forename: Jaime D
authors.initials: JD
authors.lastname: Dittman
authors.forename: Steven A
authors.initials: SA
authors.lastname: Whitham
mesh_terms: D020868:Gene Silencing; D010442:Peptide Chain Initiation, Translational; D017800:Potyvirus / Q000254:growth & development / Q000502:physiology*; D038601:Ribosomal Protein S6 / Q000096:biosynthesis*; D014026:Nicotiana / Q000821:virology*; D017898:Tobamovirus / Q000254:growth & development / Q000502:physiology*
chemical_list: D038601:Ribosomal Protein S6