title: Protective effect of an extract from Periplaneta americana on hematopoiesis in irradiated rats.
abstract: OBJECTIVE
  To investigate the protective effect of W(11)-a(12), an extract from Periplaneta americana, on hematopoiesis in irradiated rats.
  
  
  METHODS
  Wistar rats receiving total body irradiation of (60)Co gamma-rays alone or with combined radiation and skin wound injury were used in this study. W(11)-a(12) was applied either topically into the skin wounds or systemically by intraperitoneal injection. The numbers of white blood cells in peripheral blood, the nucleated cells and the colony-forming unit of granulocyte/macrophage progenitors (CFU-GM) in bone marrow were measured, respectively.
  
  
  RESULTS
  Topical application of W(11)-a(12) into skin wounds in rats with combined 6 Gy total body irradiation and skin wound injury could increase the neutrophils and macrophages in the wounded area and the nucleated cells in bone marrow at 24 h and 48 h, while the peripheral white blood cells did not show significant change. However, in rats with 4 Gy total body irradiation alone, the peripheral white blood cells, bone marrow nucleated cells and the number of colony-forming unit of granulocyte-macrophage progenitors were all significantly higher in the treatment groups by intraperitoneal injection of W(11)-a(12) than those in the control groups by injection of normal saline at days 3 and days 5 after radiation.
  
  
  CONCLUSIONS
  W(11)-a(12) showed a protective effect on hematopoiesis after total body irradiation and could increase the inflammatory cells in wounded tissues at the initiation stage after irradiation, which will benefit the management of combined radiation and skin wound injury.
authors.affiliation: State Key Laboratory of Trauma, Burns and Combined Injury, Institute of Combined Injury of PLA, Chongqing, China.
authors.forename: Xiao-Hong
authors.initials: XH
authors.lastname: Chen
authors.forename: Xin-Ze
authors.initials: XZ
authors.lastname: Ran
authors.forename: Ren-Shan
authors.initials: RS
authors.lastname: Sun
authors.forename: Chun-Meng
authors.initials: CM
authors.lastname: Shi
authors.forename: Yongping
authors.initials: Y
authors.lastname: Su
authors.forename: Chao-Hua
authors.initials: CH
authors.lastname: Guo
authors.forename: Tian-Min
authors.initials: TM
authors.lastname: Cheng
mesh_terms: D000818:Animals; D006410:Hematopoiesis / Q000187:drug effects / Q000528:radiation effects*; D020556:Neutrophil Infiltration / Q000528:radiation effects; D010526:Periplaneta; D010936:Plant Extracts / Q000494:pharmacology*; D011837:Radiation-Protective Agents / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D014916:Whole-Body Irradiation; D014945:Wound Healing / Q000187:drug effects
chemical_list: D010936:Plant Extracts; D011837:Radiation-Protective Agents