Changes in phenotype of monocyte-like THP-1 cells associated with transendothelial migration.
The level of expression of CD11b and HLA-DR surface molecules on monocyte-like THP-1 cells increased significantly as a result of transmigration of these cells through a monolayer of endothelial cells. The expression of all studied markers (CD11b, HLA-DR, and CD-14) increased significantly after transendothelial migration in the presence of TNF-alpha and IFN-gamma. The changes in surface phenotype of THP-1 cells after transendothelial migration in the presence of TNF-alpha were more pronounced than in the presence of IFN-gamma. Transendothelial migration of THP-1 cells in the presence of IL-4 caused less pronounced changes in the surface phenotype, which did not differ from changes in transmigration without cytokines.
Starikova
E A
EA
Institute of Experimental Medicine, Russian Academy of Medical Sciences, St. Petersburg, Russia. starickova@yandex.ru
Sokolov
D I
DI
Chernova
A A
AA
Freidlin
I S
IS
D039481:CD11b Antigen / Q000378:metabolism; D002460:Cell Line; D002465:Cell Movement / Q000187:drug effects; D042783:Endothelial Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D006684:HLA-DR Antigens / Q000378:metabolism; D006801:Humans; D007371:Interferon-gamma / Q000494:pharmacology; D015847:Interleukin-4 / Q000494:pharmacology; D018950:Lipopolysaccharide Receptors / Q000378:metabolism; D008264:Macrophages / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism*; D010641:Phenotype; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology
D039481:CD11b Antigen; D006684:HLA-DR Antigens; D018950:Lipopolysaccharide Receptors; D014409:Tumor Necrosis Factor-alpha; D015847:Interleukin-4; D007371:Interferon-gamma