title: Composite electrospun scaffolds for engineering tubular bone grafts.
abstract: In this study, poly (epsilon-caprolactone) [PCL] and its collagen composite blend (PCL/Col) were fabricated to scaffolds using electrospinning method. Incorporated collagen was present on the surface of the fibers, and it modulated the attachment and proliferation of pig bone marrow mesenchymal cells (pBMMCs). Osteogenic differentiation markers were more pronounced when these cells were cultured on PCL/Col fibrous meshes, as determined by immunohistochemistry for collagen type I, osteopontin, and osteocalcin. Matrix mineralization was observed only on osteogenically induced PCL/Col constructs. Long bone analogs were created by wrapping osteogenic cell sheets around the PCL/Col meshes to form hollow cylindrical cell-scaffold constructs. Culturing these constructs under dynamic conditions enhanced bone-like tissue formation and mechanical strength. We conclude that electrospun PCL/Col mesh is a promising material for bone engineering applications. Its combination with osteogenic cell sheets offers a novel and promising strategy for engineering of tubular bone analogs.
authors.affiliation: Graduate Program in Bioengineering, National University of Singapore, Singapore.
authors.forename: Andrew Krishna
authors.initials: AK
authors.lastname: Ekaputra
authors.forename: Yefang
authors.initials: Y
authors.lastname: Zhou
authors.forename: Simon McKenzie
authors.initials: SM
authors.lastname: Cool
authors.forename: Dietmar Werner
authors.initials: DW
authors.lastname: Hutmacher
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology / Q000187:drug effects; D016025:Bone Transplantation; D001842:Bone and Bones / Q000166:cytology / Q000187:drug effects / Q000502:physiology; D002208:Caproates / Q000494:pharmacology; D048430:Cell Shape / Q000187:drug effects; D002478:Cells, Cultured; D024042:Collagen Type I / Q000494:pharmacology; D007150:Immunohistochemistry; D007783:Lactones / Q000494:pharmacology; D008648:Mesoderm / Q000166:cytology; D008855:Microscopy, Electron, Scanning; D034421:Sus scrofa; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D002208:Caproates; D024042:Collagen Type I; D007783:Lactones; C121056:caprolactone