title: Activity of lysosomal exoglycosidases in serum and synovial fluid in patients with chronic Lyme and rheumatoid arthritis.
abstract: Lysosomal exoglycosidases participate in the destruction of the articular cartilage by cleaving glycoside bonds in glycoproteins and proteoglycans. The aim of the study was to determine the activity of exoglycosidases: hexosaminidase, beta-glucuronidase, beta-galactosidase, alpha-mannosidase and alpha-fucosidase in serum and synovial fluid of patients with Lyme and rheumatoid arthritis. The study group consisted of 10 patients with chronic Lyme arthritis (age 18 - 74 y), 13 with rheumatoid arthritis (age 32 - 70 y) and 10 with juvenile idiopathic arthritis (age 8 - 17 y). The control group consisted of 9 healthy volunteers (age 24 - 62 y). The activity of the exoglycosidases was determined with the p-nitrophenyl derivatives of sugars as substrates. A significant increase of the activity of all the exoglycosidases in serum and in synovial fluid of the patients with different forms of arthritis was found. The ratio of synovial fluid/serum activity of exoglycosidases was above 2.0 in LA but not in JIA and RA patients. As the main source of exoglycosidases in the joint is the synovial membrane, this result supports the appropriateness of therapeutic synovectomy in chronic Lyme arthritis with knee effusion. The serum activity of hexosaminidase may be used in monitoring the course of Lyme arthritis and the efficiency of treatment.
authors.affiliation: Department of Infectious Diseases, Medical University of Bial ystok, Bial ystok, Poland.
authors.forename: Slawomir
authors.initials: S
authors.lastname: Pancewicz
authors.forename: Janusz
authors.initials: J
authors.lastname: Popko
authors.forename: Ryszard
authors.initials: R
authors.lastname: Rutkowski
authors.forename: Malgorzata
authors.initials: M
authors.lastname: Knaś
authors.forename: Sambor
authors.initials: S
authors.lastname: Grygorczuk
authors.forename: Tomasz
authors.initials: T
authors.lastname: Guszczyn
authors.forename: Marta
authors.initials: M
authors.lastname: Bruczko
authors.forename: Slawomir
authors.initials: S
authors.lastname: Szajda
authors.forename: Joanna
authors.initials: J
authors.lastname: Zajkowska
authors.forename: Maciej
authors.initials: M
authors.lastname: Kondrusik
authors.forename: Stanislaw
authors.initials: S
authors.lastname: Sierakowski
authors.forename: Krzysztof
authors.initials: K
authors.lastname: Zwierz
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D001168:Arthritis / Q000473:pathology*; D001172:Arthritis, Rheumatoid / Q000473:pathology*; D015415:Biomarkers; D002863:Chromogenic Compounds; D005260:Female; D006026:Glycoside Hydrolases / Q000378:metabolism*; D006801:Humans; D008193:Lyme Disease / Q000150:complications / Q000473:pathology*; D008247:Lysosomes / Q000201:enzymology*; D008297:Male; D008875:Middle Aged; D044967:Serum / Q000201:enzymology*; D013582:Synovial Fluid / Q000201:enzymology*; D055815:Young Adult
chemical_list: D015415:Biomarkers; D002863:Chromogenic Compounds; D006026:Glycoside Hydrolases