[The role of p53 and NF-kappaB transcription factors in redox-dependent dysregulation of mononuclear leukocyte apoptosis].
The aim of this work was to study programmed death of blood mononuclear leukocytes taken from healthy donors and patients with acute inflammatory diseases (acute appendicitis, community-acquired pneumonia). Cellular p53 and NF-kappaB transcription factors were detected by western blotting. Active form of NF-kappaB was shown to appear in mononuclear leukocytes undergoing oxidative stress in experiment and during acute inflammation, p53 was found only under oxidative stress conditions in vitro. Despite enhanced expression of target gene mRNA of these transcription factors in oxidative stress (proapoptotic protein Bax and antiapoptotic protein Bcl-XL), the resulting vector of p53 and NF-kappaB activation is stimulation of cell's apoptotic reaction.
Novitskiĭ
V V
VV
Riazantseva
N V
NV
Chasovskikh
N Iu
NIu
Starikova
E G
EG
Kaĭgorodova
E V
EV
Starikov
Iu V
IuV
Filipenko
M L
ML
Boiarskikh
U A
UA
D000208:Acute Disease; D000293:Adolescent; D000328:Adult; D017209:Apoptosis; D001064:Appendicitis / Q000097:blood / Q000378:metabolism; D001782:Blood Donors; D003141:Communicable Diseases / Q000097:blood; D005260:Female; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000166:cytology* / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D016328:NF-kappa B / Q000502:physiology*; D018384:Oxidative Stress; D011014:Pneumonia / Q000097:blood / Q000378:metabolism; D016159:Tumor Suppressor Protein p53 / Q000502:physiology*; D055815:Young Adult
D016328:NF-kappa B; C495901:TP53 protein, human; D016159:Tumor Suppressor Protein p53