title: Platform for controlled supramolecular nanoassembly.
abstract: We here present a two-dimensional (2D) micro/nano-fluidic technique where reactant-doped liquid-crystal films spread and mix on micro- and nanopatterned substrates. Surface-supported phospholipid monolayers are individually doped with complementary DNA molecules which hybridize when these lipid films mix. Using lipid films to convey reactants reduces the dimensionality of traditional 3D chemistry to 2D, and possibly to 1D by confining the lipid film to nanometer-sized lanes. The hybridization event was observed by FRET using single-molecule-sensitive confocal fluorescence detection. We could successfully detect hybridization in lipid streams on 250 nm wide lanes. Our results show that the number and density of reactants as well as sequence of reactant addition can be controlled within confined liquid crystal films, providing a platform for nanochemistry with potential for kinetic control.
authors.affiliation: Department of Chemical and Biological Engineering, Chalmers University of Technology, 412 96, Goteborg, Sweden.
authors.forename: Ilja
authors.initials: I
authors.lastname: Czolkos
authors.forename: Jonas K
authors.initials: JK
authors.lastname: Hannestad
authors.forename: Aldo
authors.initials: A
authors.lastname: Jesorka
authors.forename: Ravindra
authors.initials: R
authors.lastname: Kumar
authors.forename: Tom
authors.initials: T
authors.lastname: Brown
authors.forename: Bo
authors.initials: B
authors.lastname: Albinsson
authors.forename: Owe
authors.initials: O
authors.lastname: Orwar
mesh_terms: D004247:DNA / Q000737:chemistry*; D050866:Liquid Crystals / Q000737:chemistry*; D008567:Membranes, Artificial; D044085:Microfluidics / Q000379:methods*; D036103:Nanotechnology / Q000379:methods*; D009693:Nucleic Acid Hybridization / Q000379:methods*; D010743:Phospholipids / Q000737:chemistry*; D013499:Surface Properties
chemical_list: D008567:Membranes, Artificial; D010743:Phospholipids; D004247:DNA