title: Visualizing intermolecular interactions in T cells.
abstract: The use of appropriate fluorescent proteins has allowed the use of FRET microscopy for investigation of intermolecular interactions in living cells. This method has the advantage of both being dynamic and of working at the subcellular level, so that the time and place where proteins interact can be visualized. We have used FRET microscopy to analyze the interactions between the T cell antigen receptor and the coreceptors CD4 and CD8. This chapter reviews data on how these coreceptors are recruited to the immunological synapse, and how they interact when the T cell is stimulated by different ligands.
authors.affiliation: Department of Immunology and Microbial Science, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA. Gascoigne@scripps.edu
authors.forename: Nicholas R J
authors.initials: NR
authors.lastname: Gascoigne
authors.forename: Jeanette
authors.initials: J
authors.lastname: Ampudia
authors.forename: Jean-Pierre
authors.initials: JP
authors.lastname: Clamme
authors.forename: Guo
authors.initials: G
authors.lastname: Fu
authors.forename: Carina
authors.initials: C
authors.lastname: Lotz
authors.forename: Michel
authors.initials: M
authors.lastname: Mallaun
authors.forename: Nathalie
authors.initials: N
authors.lastname: Niederberger
authors.forename: Ed
authors.initials: E
authors.lastname: Palmer
authors.forename: Vasily
authors.initials: V
authors.lastname: Rybakin
authors.forename: Pia P
authors.initials: PP
authors.lastname: Yachi
authors.forename: Tomasz
authors.initials: T
authors.lastname: Zal
mesh_terms: D000818:Animals; D017951:Antigen Presentation; D015704:CD4 Antigens / Q000378:metabolism*; D016827:CD8 Antigens / Q000378:metabolism*; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods*; D006801:Humans; D043942:Nanotubes; D011948:Receptors, Antigen, T-Cell / Q000378:metabolism*; D013601:T-Lymphocytes / Q000276:immunology* / Q000378:metabolism
chemical_list: D015704:CD4 Antigens; D016827:CD8 Antigens; D011948:Receptors, Antigen, T-Cell