title: Chemotactic properties and absence of the formyl peptide receptor in ferret (Mustela putorius furo) neutrophils.
abstract: This study describes a chemotaxis assay of ferret polymorphonuclear cells (PMNs). The optimal conditions for this chemotaxis assay were investigated for three chemoattractants: zymosan activated serum (ZAS), recombinant human interleukin-8 (rhIL-8) and N-formyl-Met-Leu- Phe (fMLF). In this study, ferret polymorphonuclear cells (PMNs) reacted to ZAS and rhIL-8, but not fMLF. The optimal concentration of ZAS and rhIL-8 were 5% and 100 ng/ml, respectively. The optimal incubation time of each reagent was 60 min. Due to the lack of response shown from fMLF, the existence of formyl peptide receptors (FPR) on ferret PMNs was investigated by evaluating FPR binding using flow cytometry. The receptor was not detected, implying that ferret neutrophils may lack FPR. This study confirms the fundamental experimental conditions for ferret PMNs chemotaxis and elucidates new findings concerning FPR in ferret neutrophils.
authors.affiliation: Nihon University, Veterinary Research Center, Fujisawa, Kanagawa 252-8510, Japan. azu05pooh@hotmail.com
authors.forename: Makoto
authors.initials: M
authors.lastname: Nakata
authors.forename: Kouji
authors.initials: K
authors.lastname: Otsubo
authors.forename: Tomoko
authors.initials: T
authors.lastname: Kikuchi
authors.forename: Takuya
authors.initials: T
authors.lastname: Itou
authors.forename: Takeo
authors.initials: T
authors.lastname: Sakai
mesh_terms: D000818:Animals; D002634:Chemotaxis, Leukocyte / Q000502:physiology*; D005289:Ferrets / Q000276:immunology*; D005434:Flow Cytometry; D016209:Interleukin-8 / Q000378:metabolism; D008297:Male; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000378:metabolism; D009504:Neutrophils / Q000502:physiology*; D044042:Receptors, Formyl Peptide / Q000378:metabolism / Q000502:physiology*; D015054:Zymosan / Q000378:metabolism
chemical_list: D016209:Interleukin-8; D044042:Receptors, Formyl Peptide; D009240:N-Formylmethionine Leucyl-Phenylalanine; D015054:Zymosan