title: Multiplex immune serum biomarker profiling in sarcoidosis and systemic sclerosis.
abstract: Multiplex protein technology has the potential to identify biomarkers for the differentiation, classification and improved understanding of the pathogenesis of interstitial lung disease. The aim of this study was to determine whether a 30-inflammatory biomarker panel could discriminate between healthy controls, sarcoidosis and systemic sclerosis (SSc) patients independently of other clinical indicators. We also evaluated whether a panel of biomarkers could differentiate between the presence or absence of lung fibrosis in SSc patients. We measured 30 circulating biomarkers in 20 SSc patients, 21 sarcoidosis patients and 20 healthy controls using Luminex bead technology and used Fisher's discriminant function analysis to establish the groups of classification mediators. There were significant differences in median concentration measurements between study groups for 20 of the mediators but with considerable range overlap between the groups, limiting group differentiation by single analyte measurements. However, a 17-analyte biomarker model correctly classified 90% of study individuals to their respective group and another 14-biomarker panel correctly identified the presence of lung fibrosis in SSc patients. These findings, if they are corroborated by independent studies in other centres, have potential for clinical application and may generate novel insights into the modulation of immune profiles during disease evolution.
authors.affiliation: Dept of Respiratory Medicine, Royal Brompton & Harefield NHS Trust, London, UK. p.kelleher@imperial.ac.uk
authors.forename: P
authors.initials: P
authors.lastname: Beirne
authors.forename: P
authors.initials: P
authors.lastname: Pantelidis
authors.forename: P
authors.initials: P
authors.lastname: Charles
authors.forename: A U
authors.initials: AU
authors.lastname: Wells
authors.forename: D J
authors.initials: DJ
authors.lastname: Abraham
authors.forename: C P
authors.initials: CP
authors.lastname: Denton
authors.forename: K I
authors.initials: KI
authors.lastname: Welsh
authors.forename: P L
authors.initials: PL
authors.lastname: Shah
authors.forename: R M
authors.initials: RM
authors.lastname: du Bois
authors.forename: P
authors.initials: P
authors.lastname: Kelleher
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D015415:Biomarkers / Q000378:metabolism*; D016022:Case-Control Studies; D005260:Female; D005355:Fibrosis / Q000097:blood; D006801:Humans; D007107:Immune System; D036341:Intercellular Signaling Peptides and Proteins / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D015272:Pulmonary Medicine / Q000379:methods*; D012507:Sarcoidosis / Q000097:blood*; D012595:Scleroderma, Systemic / Q000097:blood*
chemical_list: D015415:Biomarkers; D036341:Intercellular Signaling Peptides and Proteins