[Effects of hydroxy safflor yellow A on blood vessel and mRNA expression with VEGF and bFGF of transplantation tumor with gastric adenocarcinoma cell line BGC-823 in nude mice].
OBJECTIVE
To investigate the effects of Hydroxy Safflor yellow A (HSYA) on the growth of blood vessel of transplantation tumor of gastric adenocarcinoma cell line BGC-823 in nude mice and its underlying mechanism of antagonizing tumor angiogenesis.


METHODS
The BGC-823 cells was subcutaneouly injected into the right anterior armpit of BALB/C nu/nu nude mice and established the animal model of transplantation tumor. Then nude mice were divided into 4 groups at random: model group, control group, high or low dosage of HSYA group. The model group were treated with normal sodium by intraperitoneal injection, HSYA groups were treated with HSYA at concentration of 0.056 g x L(-1) and 0.028 g x L(-1) by intraperitoneal injection, and in these groups each mouse was injected 2 times everyday with 0.2 mL by 4-6 hours interval. The control group were injected in enterocoelia 1 times every 2 days starting from the third day with cytoxan at 2 g x L(-1). 20 days later, the volume and weight of nude mice were observed. The pathological change of tumor tissue was observed under optical microscope. The mRNA expression of VEGF and bFGF of transplantation tumor were detected by real time quantitative PCR.


RESULTS
The volume (607.42 +/- 252.96) mm3, weight (0.88 +/- 0.14) g of transplantation tumor, the mRNA expression level of VEGF 0.49 +/- 0.13 and bFGF 0.60 +/- 0.48 are reduced significantly after treatment with HSYA at the concentration of 0.028 g x L(-1) compared with physiologic saline-treated group (P < 0.05 or P < 0.01). The tumor pathological angiogenesis of HSYA group is also less obvious than the normal sodium-treated group.


CONCLUSIONS
HSYA in given concentration can inhibit the growth of BGC-823 transplantation tumor, and decreasing the mRNA expression of VEGF and bFGF, which suggests that inhibiting tumor angiogenesis may be one of the mechanisms of HSYA antagonizing tumor.
Xi
Shengyan
S
School of Preclinical, Beijing University of Chinese Medicine, Beijing 100029, China.
Zhang
Qian
Q
Xie
Hua
H
Liu
Lintao
L
Liu
Chaoyang
C
Gao
Xuemin
X
Zhang
Jianjun
J
Wu
Likun
L
Qian
Lili
L
Deng
Xiaoying
X
D000230:Adenocarcinoma / Q000188:drug therapy* / Q000235:genetics / Q000378:metabolism; D020533:Angiogenesis Inhibitors / Q000008:administration & dosage*; D000818:Animals; D001808:Blood Vessels / Q000187:drug effects; D045744:Cell Line, Tumor; D002599:Chalcone / Q000008:administration & dosage / Q000031:analogs & derivatives*; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage; D005260:Female; D005346:Fibroblast Growth Factors / Q000235:genetics* / Q000378:metabolism; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects*; D006801:Humans; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D011809:Quinones / Q000008:administration & dosage*; D011897:Random Allocation; D013274:Stomach Neoplasms / Q000188:drug therapy* / Q000235:genetics / Q000378:metabolism; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics* / Q000378:metabolism
D020533:Angiogenesis Inhibitors; D004365:Drugs, Chinese Herbal; D011809:Quinones; D042461:Vascular Endothelial Growth Factor A; C467480:vascular endothelial growth factor A, mouse; C085278:hydroxysafflor yellow A; D002599:Chalcone; D005346:Fibroblast Growth Factors