A stopped flow system with hydrodynamic injection for red blood cells osmotic fragility test: possibility for automatic screening of beta-thalassemia trait.
Simple instrumentation and procedure with stopped flow system coupled to hydrodynamic injection and spectrophotometric detection were developed for automatic osmotic fragility test (OFT). OFT is a test for abnormal red blood cells based on the kinetics of their rupturing in a hypotonic saline solution. A portion of red cells was merged on-line with a hypotonic saline solution. They were mixed while flowing into the detector and were stopped for a short period for continuously monitoring the change of turbidity based on a transmission signal. In this work, a possible application of the system for screening of beta-thalassemia trait is demonstrated. Descriptions of instrumentation development and parameter optimization are presented. The system offers advantages over the conventional batch-wise OFT in terms of automation, precision, analysis time and sample volume.
Khonyoung
Supada
S
Department of Chemistry and Center for Innovation in Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand.
Kradtap Hartwell
Supaporn
S
Jakmunee
Jaroon
J
Lapanantnoppakhun
Somchai
S
Sanguansermsri
Torpong
T
Grudpan
Kate
K
D004912:Erythrocytes / Q000378:metabolism*; D017022:Flow Injection Analysis / Q000295:instrumentation / Q000379:methods*; D006801:Humans; D009996:Osmotic Fragility; D012680:Sensitivity and Specificity; D013053:Spectrophotometry; D017086:beta-Thalassemia / Q000175:diagnosis* / Q000473:pathology*