Cytoskeleton and mitochondrial morphology of saprotrophs and the pathogen Heterobasidion annosum in the presence of Suillus bovinus metabolites.
Ectomycorrhizal fungi are known to synthesize antifungal compounds both in vitro and in symbiosis with the host-plants. Culture filtrates of the ectomycorrhizal fungus Suillus bovinus (at pHs of 2.5-6) showed antifungal activity towards saprotrophs Trichoderma harzianum, and Trichoderma virens and the pathogen Heterobasidion annosum, by significantly suppressing their growth relative to sterile liquid medium at the same pHs. In the presence of the culture filtrates, hyphae of the saprotrophs and the pathogen were characterized by distensions, irregular and frequent branching, tip damage and cytoplasm coagulation. Since hyphal abnormalities may be evoked by disruptions in the cytoskeleton and mitochondria, their structural changes were also examined. Depolymerization of microtubules was confirmed for all of the fungi. Serious damage to mitochondria morphology may cause significant functional impairment. Growth of mycelia was inhibited in the lower pH S. bovinus culture filtrate, and the mitochondrial morphology was altered. This suggests that the activity of antifungal compounds synthesized by ectomycorrhizal fungus is significantly affected by pH.
Mucha
Joanna
J
Laboratory of Root System Pathology, Institute of Dendrology, Polish Academy of Science, Parkowa 5, 62-035 Kórnik, Poland. mucha_joanna@wp.pl
Zadworny
Marcin
M
Werner
Antoni
A
D000363:Agaricales / Q000166:cytology* / Q000254:growth & development / Q000378:metabolism*; D000935:Antifungal Agents / Q000737:chemistry / Q000378:metabolism; D003470:Culture Media / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D025301:Hyphae / Q000166:cytology / Q000254:growth & development / Q000378:metabolism; D008870:Microtubules / Q000378:metabolism / Q000648:ultrastructure*; D008928:Mitochondria / Q000378:metabolism / Q000648:ultrastructure*; D038821:Mycorrhizae / Q000378:metabolism*; D010935:Plant Diseases / Q000382:microbiology; D014242:Trichoderma / Q000166:cytology / Q000254:growth & development / Q000378:metabolism
D000935:Antifungal Agents; D003470:Culture Media