Investigation of the elasticity of a cisplatin-DNA adduct via single-molecule measurements and bimodal modeling.
Cisplatin has been known as an anticancer drug for a long time. It is therapeutically active upon binding to DNA. A double-bound cisplatin bends DNA into a localized kink. We model the elastic properties of cisplatin-DNA adducts at moderate tension (<6 pN). It is shown that from the mechanical point of view the action of cisplatin can be revealed by reduced persistence length. We derived two expressions for the persistence length, which apply in the linear-response and the strong-force regimes, respectively. Experimental data for DNA adducts stretched by magnetic tweezers are consistently fitted by these expressions. This allows us to estimate the degree of platination at various salt concentrations.
Lee
Nam-Kyung
NK
Department of Physics, Institute of Fundamental Physics, Sejong University, Seoul 143-743, Korea. lee@sejong.ac.kr
Park
Jin-Sung
JS
Johner
Albert
A
Obukhov
Sergei
S
Hyon
Ju-Yong
JY
Lee
Kyoung J
KJ
Hong
Seok-Cheol
SC
D000465:Algorithms; D000970:Antineoplastic Agents / Q000737:chemistry; D002945:Cisplatin / Q000737:chemistry*; D004247:DNA / Q000737:chemistry; D018736:DNA Adducts / Q000737:chemistry*; D004548:Elasticity; D008958:Models, Molecular
D000970:Antineoplastic Agents; D018736:DNA Adducts; C057270:cisplatin-DNA adduct; D004247:DNA; D002945:Cisplatin