Functional analysis of an Orc6 mutant in Drosophila.
The origin recognition complex (ORC) is a 6-subunit complex required for the initiation of DNA replication in eukaryotic organisms. ORC is also involved in other cell functions. The smallest Drosophila ORC subunit, Orc6, is important for both DNA replication and cytokinesis. To study the role of Orc6 in vivo, the orc6 gene was deleted by imprecise excision of P element. Lethal alleles of orc6 are defective in DNA replication and also show abnormal chromosome condensation and segregation. The analysis of cells containing the orc6 deletion revealed that they arrest in both the G(1) and mitotic stages of the cell cycle. Orc6 deletion can be rescued to viability by a full-length Orc6 transgene. The expression of mutant transgenes of Orc6 with deleted or mutated C-terminal domain results in a release of mutant cells from G(1) arrest and restoration of DNA replication, indicating that the DNA replication function of Orc6 is associated with its N-terminal domain. However, these mutant cells accumulate at mitosis, suggesting that the C-terminal domain of Orc6 is important for the passage through the M phase. In a cross-species complementation experiment, the expression of human Orc6 in Drosophila Orc6 mutant cells rescued DNA replication, suggesting that this function of the protein is conserved among metazoans.
Balasov
Maxim
M
Department of Biochemistry and Molecular Genetics, University of Alabama at Birmingham School of Medicine, Birmingham, AL 35294, USA.
Huijbregts
Richard P H
RP
Chesnokov
Igor
I
D000818:Animals; D030801:Animals, Genetically Modified; D002470:Cell Survival / Q000235:genetics / Q000502:physiology; D004261:DNA Replication / Q000235:genetics / Q000502:physiology; D004330:Drosophila / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D004331:Drosophila melanogaster / Q000235:genetics / Q000378:metabolism / Q000502:physiology; D005455:Fluorescent Antibody Technique; D016193:G1 Phase / Q000235:genetics / Q000502:physiology; D017353:Gene Deletion; D005816:Genetic Complementation Test; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D006801:Humans; D015151:Immunoblotting; D007150:Immunohistochemistry; D008938:Mitosis / Q000235:genetics / Q000502:physiology; D009154:Mutation; D009474:Neurons / Q000166:cytology / Q000378:metabolism; D051738:Origin Recognition Complex / Q000235:genetics / Q000378:metabolism / Q000502:physiology*
D029721:Drosophila Proteins; C477598:ORC6 protein, Drosophila; C497227:ORC6 protein, human; D051738:Origin Recognition Complex; D049452:Green Fluorescent Proteins