[Genotype validation of 12 STR loci by sequencing method].
OBJECTIVE
To validate the genotype of 12 STR loci by sequencing method.


METHODS
According to the special sequence of 12 STR loci (CSF1PO, FGA, TH01, TPOX, VWA, D5S818, D7S820, D8S1179, D13S317, D16S539, D18S51 and D21S11), the relative PCR primers were designed. PCR products of control DNA (9947A) and STR mutation samples were sequenced.


RESULTS
The sequencing results of both the control DNA (9947A) and STR mutation samples were consistent with their genotyping results.


CONCLUSIONS
The sequencing method developed are accurate and sensitive, can be used for the validation of STR genotyping results.
Li
Cheng-Tao
CT
Shanghai Key Laboratory of Forensic Medicine, Institute of Forensic Science, Ministry of Justice, P R China, Shanghai 200063, China. lichengtaohla@163.com
Zhao
Zhen-Min
ZM
Liu
Yan
Y
Li
Li
L
D000483:Alleles; D001483:Base Sequence; D053803:Forensic Genetics; D005838:Genotype; D006801:Humans; D008969:Molecular Sequence Data; D009154:Mutation; D010334:Paternity; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA / Q000379:methods*; D020080:Tandem Repeat Sequences / Q000235:genetics*