SELDI-TOF MS profiling of serum for detection of nasopharyngeal carcinoma.
BACKGROUND
No satisfactory biomarkers are currently available to screen for nasopharyngeal carcinoma (NPC). We have developed and evaluated surface-enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF MS) for detection and analysis of multiple proteins for distinguishing individuals with NPC from control individuals.


METHODS
A preliminary learning set and a classification tree of spectra derived from 24 patients with NPC and a group of 24 noncancer controls were used to develop a proteomic model that discriminated cancer from noncancer effectively. Then, the validity of the classification tree was challenged with a blind test set, which included another 20 patients with NPC and 12 noncancer controls.


RESULTS
A panel of 3 biomarkers ranging m/z 3-20 k was selected to establish Decision Tree model by BPS with sensitivity of 91.66% and specificity of 95.83%. The ability to detect NPC patients was evaluated, a sensitivity of 95.0% and specificity of 83.33% were validated in blind testing set.


CONCLUSIONS
This high-flux proteomic classification system will provide a highly accurate and innovative approach for the detection/diagnosis of NPC.
Huang
Yuan-Jiao
YJ
Guangxi Medical Scientific Research Center, Guangxi Medical University, Nanning, PR China. hyjgxmu@126.com
Xuan
Chao
C
Zhang
Bei-Bei
BB
Liao
Ming
M
Deng
Kai-Feng
KF
He
Min
M
Zhao
Jin-Min
JM
D014408:Biomarkers, Tumor / Q000097:blood*; D002294:Carcinoma, Squamous Cell / Q000175:diagnosis*; D016022:Case-Control Studies; D003663:Decision Trees; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009303:Nasopharyngeal Neoplasms / Q000175:diagnosis*; D009363:Neoplasm Proteins / Q000097:blood*; D040901:Proteomics; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
D014408:Biomarkers, Tumor; D009363:Neoplasm Proteins