title: Phage display selection of cyclic peptides that inhibit Andes virus infection.
abstract: Specific therapy is not available for hantavirus cardiopulmonary syndrome caused by Andes virus (ANDV). Peptides capable of blocking ANDV infection in vitro were identified using antibodies against ANDV surface glycoproteins Gn and Gc to competitively elute a cyclic nonapeptide-bearing phage display library from purified ANDV particles. Phage was examined for ANDV infection inhibition in vitro, and nonapeptides were synthesized based on the most-potent phage sequences. Three peptides showed levels of viral inhibition which were significantly increased by combination treatment with anti-Gn- and anti-Gc-targeting peptides. These peptides will be valuable tools for further development of both peptide and nonpeptide therapeutic agents.
authors.affiliation: Department of Pathology, University of New Mexico Health Sciences Center, Albuquerque, New Mexico 87131, USA.
authors.forename: Pamela R
authors.initials: PR
authors.lastname: Hall
authors.forename: Brian
authors.initials: B
authors.lastname: Hjelle
authors.forename: Hadya
authors.initials: H
authors.lastname: Njus
authors.forename: Chunyan
authors.initials: C
authors.lastname: Ye
authors.forename: Virginie
authors.initials: V
authors.lastname: Bondu-Hawkins
authors.forename: David C
authors.initials: DC
authors.lastname: Brown
authors.forename: Kathleen A
authors.initials: KA
authors.lastname: Kilpatrick
authors.forename: Richard S
authors.initials: RS
authors.lastname: Larson
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000998:Antiviral Agents / Q000138:chemical synthesis / Q000302:isolation & purification* / Q000494:pharmacology*; D002522:Chlorocebus aethiops; D006476:Orthohantavirus / Q000187:drug effects*; D006801:Humans; D008826:Microbial Sensitivity Tests; D008958:Models, Molecular; D008969:Molecular Sequence Data; D019151:Peptide Library; D010456:Peptides, Cyclic / Q000138:chemical synthesis / Q000235:genetics / Q000302:isolation & purification* / Q000494:pharmacology*; D014709:Vero Cells
chemical_list: D000998:Antiviral Agents; D019151:Peptide Library; D010456:Peptides, Cyclic