title: Stromal CD10 expression, as well as increased dermal macrophages and decreased Langerhans cells, are associated with malignant transformation of keratinocytes.
abstract: BACKGROUND
  It has become evident that resident stromal cells, such as fibroblasts and inflammatory cells, are involved in the metastatic process, including proliferation or migration of malignant neoplasms. We analyzed CD10+ stromal cells, dermal macrophages and Langerhans cells (LCs) in skin tumors.
  
  
  METHODS
  Immunohistological staining was performed with markers for macrophages (CD68), LC (CD1a), stromal fibroblasts (CD10) and cell proliferation (Ki67) in 12 normal skins (NSs) and 15 cases each of seborrheic keratosis (SK), actinic keratosis (AK), keratoacanthoma (KA), Bowen's disease (BD) and squamous cell carcinoma (SCC).
  
  
  RESULTS
  All SCCs showed weak to strong stromal CD10 expression, while all NS, SK and AK were negative. Weak CD10 expression was observed in only 2 of 15 samples in both BD and KA. The number of CD68+ cells and Ki67 labeling index in SCC and BD were significantly higher than that in KA, AK and SK. In contrast, the number of LC was lower in SCC and BD. The stromal CD10 expression was significantly correlated with the Ki67 labeling indices and CD68+ cells and negatively correlated with decreased LC.
  
  
  CONCLUSIONS
  The stromal CD10 expression is associated with malignant transformation of keratinocytes together with infiltration of dermal macrophages and loss of LC.
authors.affiliation: Department of Dermatology, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan. mtakahara@dermatol.med.kyushu-u.ac.jp
authors.forename: Masakazu
authors.initials: M
authors.lastname: Takahara
authors.forename: Shanjuan
authors.initials: S
authors.lastname: Chen
authors.forename: Makiko
authors.initials: M
authors.lastname: Kido
authors.forename: Satoshi
authors.initials: S
authors.lastname: Takeuchi
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Uchi
authors.forename: Yating
authors.initials: Y
authors.lastname: Tu
authors.forename: Yoichi
authors.initials: Y
authors.lastname: Moroi
authors.forename: Masutaka
authors.initials: M
authors.lastname: Furue
mesh_terms: D001913:Bowen's Disease / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D002294:Carcinoma, Squamous Cell / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D002471:Cell Transformation, Neoplastic / Q000276:immunology / Q000378:metabolism / Q000473:pathology*; D003238:Connective Tissue / Q000276:immunology / Q000378:metabolism; D005347:Fibroblasts / Q000378:metabolism / Q000473:pathology; D006801:Humans; D007150:Immunohistochemistry; D015603:Keratinocytes / Q000276:immunology / Q000378:metabolism / Q000473:pathology*; D007636:Keratoacanthoma / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D055623:Keratosis, Actinic / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D017492:Keratosis, Seborrheic / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D007801:Langerhans Cells / Q000276:immunology / Q000473:pathology*; D008264:Macrophages / Q000276:immunology / Q000473:pathology*; D015260:Neprilysin / Q000096:biosynthesis*; D012867:Skin / Q000166:cytology; D012871:Skin Diseases / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D012878:Skin Neoplasms / Q000276:immunology / Q000378:metabolism / Q000473:pathology*; D017154:Stromal Cells / Q000378:metabolism / Q000473:pathology
chemical_list: D015260:Neprilysin