A flow-injection chemiluminescent method for the evaluation of the antioxidant activity of 5'-nucleotides.
In the present work, a simple chemiluminescence (CL) method coupled with flow-injection analysis for the evaluation of antioxidant activity of 5'-nucleotides (5'-AMP, 5'-CMP, 5'-GMP, 5'-UMP) was proposed. It is based on inhibition effect of the studied substances on CL emission of luminol-potassium ferricyanide-pyrogallol. Experiments were performed to evaluate the nature of the inhibition by 5'-nucleotides of the CL reaction and their antioxidant activities. Based on the experimental results, it was observed that 5'-nucleotides are available antioxidants that could effectively scavenge superoxide anion free radicals in a concentration-dependent way. This will provide a basis for further development of the use of nucleotides.
Wang
Zhouping
Z
State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China. wangzp1974@hotmail.com
Xu
Huan
H
Qian
Jia
J
Ye
Jing
J
Yang
Zhen
Z
Sun
Huachao
H
Shi
Yonghui
Y
D000975:Antioxidants / Q000737:chemistry*; D005292:Ferricyanides / Q000737:chemistry; D017022:Flow Injection Analysis; D016166:Free Radical Scavengers / Q000737:chemistry; D008163:Luminescent Measurements / Q000379:methods*; D008165:Luminol / Q000737:chemistry; D009711:Nucleotides / Q000737:chemistry*; D011748:Pyrogallol / Q000737:chemistry; D013481:Superoxides / Q000737:chemistry
D000975:Antioxidants; D005292:Ferricyanides; D016166:Free Radical Scavengers; D009711:Nucleotides; D011748:Pyrogallol; D013481:Superoxides; D008165:Luminol; C028033:potassium ferricyanide