Multiple dual-mode centrifugal partition chromatography as an efficient method for the purification of a mycosporine from a crude methanolic extract of Lichina pygmaea.
Centrifugal partition chromatography method was applied to the separation and purification of a crude methanolic extract of a cyanobacterial lichen, Lichina pygmaea. A multiple dual-mode was used to separate two compounds of interest, namely mycosporine-serinol and a glutamic acid derivative. These compounds are described here for the first time in a lichen. Their structures were identified by UV, IR, ESI-MS, (1)H NMR, (13)C NMR, and 2D NMR.
Roullier
Catherine
C
EA 4090 Substances lichéniques et photoprotection, UFR Sciences pharmaceutiques et Biologiques, Université Européenne de Bretagne, Université de Rennes 1, 2 Av. du Pr. Léon Bernard, 35043 Rennes Cedex, France.
Chollet-Krugler
Marylène
M
Bernard
Aurélie
A
Boustie
Joël
J
D001203:Ascomycota / Q000737:chemistry*; D002853:Chromatography, Liquid / Q000379:methods*; D000458:Cyanobacteria / Q000737:chemistry*; D003512:Cyclohexanones / Q000737:chemistry / Q000302:isolation & purification*; D008011:Lichens / Q000737:chemistry*; D000432:Methanol / Q000737:chemistry; D011409:Propylene Glycols / Q000737:chemistry / Q000302:isolation & purification*
D003512:Cyclohexanones; D011409:Propylene Glycols; C544070:mycosporine-serinol; D000432:Methanol