[Effects of anti-heparanase antibody on the growth and invasion of HCCLM6 human hepatocellular carcinoma cells].
OBJECTIVE
To investigate the effects of the self-developed anti-heparanase polypeptide antibodies on growth and invasion of human hepatocellular carcinoma HCCLM6 cells.


METHODS
Using MTT, flow cytometry, plate clone formation, transwell invasion and heparan degrading enzyme assay, the growth and invasion changes of human hepatocellular carcinoma HCCLM6 cells by co-culture with each of three self-developed rabbit anti-heparanase polyclonal antibodies were detected.


RESULTS
Compared with normal rabbit IgG, in the presence of each anti-heparanase polypeptide antibody, the growth, cell cycle and clone formation remained unchanged, and under the P1 or P2 anti-heparanase polypeptide antibody (with final concentration 100 microg/ml), the cell invasiveness was inhibited by 52.5% and 36.6%, respectively, and the heparanase activity was inhibited by 42.9% and 39.1%, respectively.


CONCLUSIONS
The P1 and P2 anti-heparanase polypeptide antibodies can effectively inhibit the invasion ability and heparanase activity of liver cancer HCCLM6 cells. However, All the three antibodies have no effects on its growth, cell cycle and clone formation.
Han
Xiao-mei
XM
Department of Gastroenterology, Zhejiang Provincial Key Gastroenterology Laboratory, Zhejiang Provincial People's Hospital, Hangzhou 310014, China.
Wang
Hui-ju
HJ
Yang
Jian-min
JM
Zhou
Yong-lie
YL
Tao
Hou-quan
HQ
Gao
Han
H
Ye
Zai-yuan
ZY
D000906:Antibodies / Q000494:pharmacology; D006528:Carcinoma, Hepatocellular / Q000201:enzymology / Q000473:pathology*; D002448:Cell Adhesion; D002454:Cell Differentiation; D045744:Cell Line, Tumor; D002465:Cell Movement; D018920:Coculture Techniques; D004789:Enzyme Activation; D005966:Glucuronidase / Q000276:immunology* / Q000378:metabolism; D006801:Humans; D008113:Liver Neoplasms / Q000201:enzymology / Q000473:pathology*; D009361:Neoplasm Invasiveness; D000099175:Heparanase
D000906:Antibodies; D005966:Glucuronidase; D000099175:Heparanase