Relevance of testicular sperm DNA oxidation for the outcome of ovum donation cycles.
OBJECTIVE
To determine the relevance of sperm DNA oxidation caused by free radicals in samples obtained via testicular biopsies by means of flow cytometry by correlating the measurements of 8-hydroxy-2'-deoxyguanosine (8-OHdG) with embryo features and pregnancy achievement.


METHODS
Prospective cross-sectional study.


METHODS
Private University-affiliated setting.


METHODS
Fifty-seven azoospermic patients undergoing testicular sperm extraction (TESE) were analyzed in their corresponding assisted reporductive technology cycles using ovum donation to standardize female's characteristics.


METHODS
None.


METHODS
Quantification of the adduct 8-OHdG in testicular tissue samples, and its effect on markers of embryo quality and reproductive success, and its relevance as marker of TESE sperm quality.


RESULTS
We found the status of sperm DNA oxidation to have very little clinical relevance for several parameters of embryo quality, fertilization rates, early (days 2-3) and late (days 5-6) development, and achievement of pregnancy.


CONCLUSIONS
The TESE obtained cells from azoospermic males do not possess a DNA oxidation status of significant importance in the success of assisted reproduction treatments, as determined by 8-OHdG measurement of each category of cell ploidy.
Aguilar
Cristóbal
C
Instituto Universitario IVI and FIVI, (Fundación IVI), Valencia, Spain.
Meseguer
Marcos
M
García-Herrero
Sandra
S
Gil-Salom
Manuel
M
O'Connor
José E
JE
Garrido
Nicolás
N
D000328:Adult; D053713:Azoospermia / Q000628:therapy; D004247:DNA / Q000378:metabolism*; D004249:DNA Damage / Q000502:physiology; D005260:Female; D006801:Humans; D008297:Male; D018587:Oocyte Donation / Q000379:methods* / Q000592:standards; D010084:Oxidation-Reduction; D018384:Oxidative Stress / Q000502:physiology*; D011003:Ploidies; D011247:Pregnancy; D020554:Sperm Injections, Intracytoplasmic / Q000379:methods* / Q000592:standards; D053625:Sperm Retrieval; D013094:Spermatozoa / Q000378:metabolism*; D016896:Treatment Outcome
D004247:DNA