title: In vivo fluorescence imaging of primate retinal ganglion cells and retinal pigment epithelial cells.
abstract: The ability to resolve single cells noninvasively in the living retina has important applications for the study of normal retina, diseased retina, and the efficacy of therapies for retinal disease. We describe a new instrument for high-resolution, in vivo imaging of the mammalian retina that combines the benefits of confocal detection, adaptive optics, multispectral, and fluorescence imaging. The instrument is capable of imaging single ganglion cells and their axons through retrograde transport in ganglion cells of fluorescent dyes injected into the monkey lateral geniculate nucleus (LGN). In addition, we demonstrate a method involving simultaneous imaging in two spectral bands that allows the integration of very weak signals across many frames despite inter-frame movement of the eye. With this method, we are also able to resolve the smallest retinal capillaries in fluorescein angiography and the mosaic of retinal pigment epithelium (RPE) cells with lipofuscin autofluorescence.
authors.affiliation: Center for Visual Science, University of Rochester, Rochester, NY 146271, USA. dgray@cvs.rochester.edu
authors.forename: Daniel C
authors.initials: DC
authors.lastname: Gray
authors.forename: William
authors.initials: W
authors.lastname: Merigan
authors.forename: Jessica I
authors.initials: JI
authors.lastname: Wolfing
authors.forename: Bernard P
authors.initials: BP
authors.lastname: Gee
authors.forename: Jason
authors.initials: J
authors.lastname: Porter
authors.forename: Alfredo
authors.initials: A
authors.lastname: Dubra
authors.forename: Ted H
authors.initials: TH
authors.lastname: Twietmeyer
authors.forename: Kamran
authors.initials: K
authors.lastname: Ahamd
authors.forename: Remy
authors.initials: R
authors.lastname: Tumbar
authors.forename: Fred
authors.initials: F
authors.lastname: Reinholz
authors.forename: David R
authors.initials: DR
authors.lastname: Williams
mesh_terms: D000818:Animals; D005456:Fluorescent Dyes; D008254:Macaca nemestrina; D008856:Microscopy, Fluorescence / Q000379:methods*; D015203:Reproducibility of Results; D012165:Retinal Ganglion Cells / Q000166:cytology*; D055213:Retinal Pigment Epithelium / Q000166:cytology*
chemical_list: D005456:Fluorescent Dyes