Two novel transposon delivery vectors based on mariner transposon for random mutagenesis of Bacillus thuringiensis.
Two new mariner transposon delivery vector systems, pMarA333 and pMarB333, had been constructed to randomly mutagenize Bacillus thuringiensis in vivo. The results showed that both systems could randomly insert into the genome of B. thuringiensis YBT881 (CCAM 020673). These systems could have potential for further construction of mutant libraries of B. thuringiensis and other Bacillus strains.
Li
Mingshun
M
State Key Laboratory of Agricultural Microbiology and National Engineering Research Center for Microbial Pesticides, Huazhong Agricultural University, Wuhan 430070, PR China.
Li
Minglei
M
Yin
Wen
W
He
Jin
J
Yu
Ziniu
Z
D001413:Bacillus thuringiensis / Q000235:genetics*; D004251:DNA Transposable Elements; D005822:Genetic Vectors; D016254:Mutagenesis, Insertional / Q000379:methods*; D010957:Plasmids
D004251:DNA Transposable Elements