Identification of a molten globule like state in HC-N fragment of botulinum neurotoxin A: shedding light on the poorly-known features of a conserved sub-domain.
C-terminal fragment of the Botulinum neurotoxin A comprises two sub-domains including H(C)-N and H(C)-C. Here, the conformational change of H(C)-N was studied by spectroscopic techniques. The results indicated that the partially unfolded state forms during unfolding of H(C)-N. This finding may shed light on poorly--known features of the protein.
Hasani
Leila
L
Department of Biophysics, Faculty of Basic Sciences, Tarbiat Modares University, Tehran, Iran.
Ranjbar
Bijan
B
Tavallaie
Mahmood
M
Sadeghizadeh
Majid
M
D019274:Botulinum Toxins, Type A / Q000737:chemistry*; D002942:Circular Dichroism; D019791:Guanidine / Q000737:chemistry; D010446:Peptide Fragments / Q000737:chemistry*; D017510:Protein Folding; D017434:Protein Structure, Tertiary; D012967:Sodium Dodecyl Sulfate / Q000737:chemistry; D013050:Spectrometry, Fluorescence
D010446:Peptide Fragments; D012967:Sodium Dodecyl Sulfate; D019274:Botulinum Toxins, Type A; D019791:Guanidine