Immunohistochemical analysis of cyclooxygenase-2 induction during wound healing in dog skin.
Cyclooxygenase (COX)-2 is an inducible isoform of COX and is expressed under abnormal health conditions. This study elucidated the cutaneous induction of COX-2 during the wound healing processes in dog skin. Dog skin was sutured after punch biopsy and investigated histologically and immunohistochemically on days 0 (normal), 1, 3, 5, 7, 10, and 14 after injury. Histological changes, including infiltration of inflammatory cells and proliferation of fibroblast-like cells, were observed as predicted, and there was a close and significant correlation between these 2 events. COX-2-positive cells were detected in the epidermis between days 1 and 7, and bimodal peaks were observed in the case of the percentage of COX-2-positive cells. In inflammatory cells, COX-positive signals were detected on day 3 only. Here, we clarified the localization and pattern of the induced COX-2 expression during wound healing in dog skin.
Hamamoto
T
T
Laboratory of Veterinary Anatomy, Department of Veterinary Medicine, Kagoshima University, Japan.
Yabuki
A
A
Yamato
O
O
Fujiki
M
M
Misumi
K
K
Matsumoto
M
M
D000818:Animals; D051546:Cyclooxygenase 2 / Q000235:genetics / Q000378:metabolism*; D004285:Dogs / Q000293:injuries* / Q000502:physiology*; D015971:Gene Expression Regulation, Enzymologic / Q000502:physiology*; D007150:Immunohistochemistry / Q000662:veterinary; D021381:Protein Transport; D012867:Skin / Q000201:enzymology*; D013997:Time Factors; D014945:Wound Healing / Q000502:physiology*
D051546:Cyclooxygenase 2