Role of protein kinase C-delta in angiotensin II induced cardiac fibrosis.
Previous studies have demonstrated a role for angiotensin II (AngII) and myofibroblasts (myoFb) in cardiac fibrosis. However, the role of PKC-delta in AngII mediated cardiac fibrosis is unclear. Therefore, the present study was designed to investigate the role of PKC-delta in AngII induced cardiac collagen expression and fibrosis. AngII treatment significantly (p<0.05) increased myoFb collagen expression, whereas PKC-delta siRNA treatment or rottlerin, a PKC-delta inhibitor abrogated (p<0.05) AngII induced collagen expression. MyoFb transfected with PKC-delta over expression vector showed significant increase (p<0.05) in the collagen expression as compared to control. Two weeks of chronic AngII infused rats showed significant (p<0.05) increase in collagen expression compared to sham operated rats. This increase in cardiac collagen expression was abrogated by rottlerin treatment. In conclusion, both in vitro and in vivo data strongly suggest a role for PKC-delta in AngII induced cardiac fibrosis.
Chintalgattu
Vishnu
V
Department of Physiology, Brody School of Medicine at East Carolina University, Greenville, NC 27834, USA.
Katwa
Laxmansa C
LC
D000098:Acetophenones / Q000494:pharmacology; D000804:Angiotensin II / Q000378:metabolism* / Q000494:pharmacology; D000818:Animals; D001578:Benzopyrans / Q000494:pharmacology; D049109:Cell Proliferation; D003094:Collagen / Q000096:biosynthesis*; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005347:Fibroblasts / Q000201:enzymology / Q000473:pathology; D005355:Fibrosis; D008297:Male; D009206:Myocardium / Q000201:enzymology / Q000473:pathology*; D051745:Protein Kinase C-delta / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D034741:RNA, Small Interfering / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley
D000098:Acetophenones; D001578:Benzopyrans; D004791:Enzyme Inhibitors; D034741:RNA, Small Interfering; D000804:Angiotensin II; D003094:Collagen; C085746:rottlerin; D051745:Protein Kinase C-delta