title: Cytogenetical damage in exfoliated oral mucosa cells in elderly people suffering denture stomatitis.
abstract: OBJECTIVE
  The aim of this study was to evaluate comparatively the DNA damage (micronucleus) and cellular death (pyknosis, karyolysis and karyorrhexis) in exfoliated oral mucosa cells from chronic denture stomatitis patients and healthy controls.
  
  
  BACKGROUND
  Over the course of ageing, individuals may develop many diseases such as denture stomatitis.
  
  
  METHODS
  A total of 23 chronic denture stomatitis patients and 23 controls presenting good oral conditions were included in this study. Individuals had epithelial cells mechanically exfoliated, placed in fixative and placed on clean slides, which were checked for nuclear phenotypes.
  
  
  RESULTS
  The results indicated no statistically significant differences (p > 0.05) of micronucleated oral mucosa cells from chronic denture stomatitis patients when compared to healthy controls. Nevertheless, chronic denture stomatitis was able to increase other nuclear alterations closely related to cytotoxicity such as karyorrhexis, pyknosis and karyolysis as depicted by significant differences (p < 0.05) between groups. No interaction was observed between smoking and chronic denture stomatitis.
  
  
  CONCLUSIONS
  In summary, these data indicated that chronic denture stomatitis was able to induce cytotoxic effects as assessed by a micronucleus test.
authors.affiliation: Department of Dental Clinics, University of Sacred Heart, USC, Bauru, SP, Brazil.
authors.forename: Mariza A
authors.initials: MA
authors.lastname: Matsumoto
authors.forename: Juliane
authors.initials: J
authors.lastname: Castanho
authors.forename: Roberto Y
authors.initials: RY
authors.lastname: Kawakami
authors.forename: Daniel A
authors.initials: DA
authors.lastname: Ribeiro
mesh_terms: D000368:Aged; D016923:Cell Death; D002467:Cell Nucleus / Q000648:ultrastructure; D002908:Chronic Disease; D004249:DNA Damage / Q000235:genetics*; D004847:Epithelial Cells / Q000473:pathology / Q000648:ultrastructure; D005260:Female; D006801:Humans; D008297:Male; D048629:Micronuclei, Chromosome-Defective / Q000145:classification; D015162:Micronucleus Tests; D008875:Middle Aged; D009061:Mouth Mucosa / Q000473:pathology* / Q000648:ultrastructure; D010641:Phenotype; D013282:Stomatitis, Denture / Q000235:genetics* / Q000473:pathology