Simple and reliable methods for the determination of sixteen marker components for quality control of Daochi pill by HPLC coupled with diode array detection.
BACKGROUND
Traditional Chinese medicine plays a very important role in the healthcare system of China and thus the quality control of medicinal herb products is of paramount concern.


OBJECTIVE
To establish a simple and effective high-performance liquid chromatography (HPLC) method to evaluate the quality of Daochi pill.


METHODS
Two HPLC methods were developed for the determination of 16 marker components in Daochi pills. In method A, the analytes were separated on a Lichrosorb C(18) column using a gradient elution of methanol and 1% aqueous phosphoric acid (pH 2.9, adjusted by triethylamine). In method B, the separation was achieved on an Agilent Eclipse Plus C(18) column using a gradient elution of methanol and 3% aqueous phosphoric acid (pH 2.0, adjusted by triethylamine) in a gradient elution mode.


RESULTS
Methods were linear over the range 0.27-500 microg/mL (r(2) >or= 0.9995). Accuracy, precision and repeatability were all within the required limits. The mean recoveries measured at three concentrations were higher than 95% with RSD <or= 4% for the targets.


CONCLUSIONS
The established methods are simple and can be used as tools for quality evaluation and control of this natural product.
Lu
Binan
B
College of Pharmacy, Dalian Medical University, Dalian, China.
Liu
Yuetao
Y
Yin
Lianhong
L
Wang
Xiaona
X
Peng
Jinyong
J
D000880:Anthraquinones / Q000032:analysis; D001565:Benzoates / Q000032:analysis; D001599:Berberine / Q000031:analogs & derivatives / Q000032:analysis; D001600:Berberine Alkaloids / Q000032:analysis; D001952:Bridged-Ring Compounds / Q000032:analysis; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation* / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000737:chemistry* / Q000592:standards; D004642:Emodin / Q000031:analogs & derivatives / Q000032:analysis; D044950:Flavanones / Q000032:analysis; D005419:Flavonoids / Q000032:analysis; D005960:Glucosides / Q000032:analysis; D039823:Iridoids / Q000032:analysis; D000432:Methanol / Q000737:chemistry; D015394:Molecular Structure; D039821:Monoterpenes; D010756:Phosphoric Acids / Q000737:chemistry; D011786:Quality Control; D015203:Reproducibility of Results; D006027:Glycosides
D000880:Anthraquinones; D001565:Benzoates; D001599:Berberine; D001600:Berberine Alkaloids; D001952:Bridged-Ring Compounds; D004365:Drugs, Chinese Herbal; D004642:Emodin; D044950:Flavanones; D005419:Flavonoids; D005960:Glucosides; D039823:Iridoids; D000432:Methanol; D039821:Monoterpenes; D010756:Phosphoric Acids; C055785:jatrorrhizine; C034384:coptisine; C007835:geniposide; C015423:peoniflorin; D006027:Glycosides; C038044:baicalin; C043561:chrysin; C006680:baicalein; C061432:epiberberine; C030242:phosphoric acid; C473995:wogonoside; C005413:palmatine; C008905:physcione; C085514:wogonin; C020491:rhein