[NO may function in the downstream of Ca2+ in ethylene induced stomatal closure in Vicia faba L].
Through pharmacological combined with laser scanning confocal microscope (LSCM) and spectrophotography to study the role of Ca2+ and NO in signaling during Vicia faba L. stomatal movement response to ethylene (Eth). The results showed that treatment with ethephon (0.004%, 0.04%, 0.4%) resulted in a time- and dose-dependent stomatal closure under light. NO scavenger cPTIO, nitrate reductase inhibitor NaN3, or extracellular Ca2+ chelation EGTA reduced ethylene-induced stomatal closure. Moreover, ethylene was shown to enhance nitric oxide levels and, corresponding, nitrate reductase activity. Inhibition of the nitrate reductase diminished ethylene-induced NO production in both stomatal guard cell and leaf. Finally, ethylene-induced NO levels and nitrate reductase activity decreased when Ca2+ was compromised. On the basis of biochemical and pharmacological experimental results, we can conclude that Ca2+ and NO were involved in the signal transduction pathway of ethylene induced stomatal closure. Nitrate reductase-derived NO may represents a novel downstream component of Ca2+ signaling cascade during ethylene-induced stomatal movement in Vicia faba L.
Liu
Guo Hua
GH
Life Sciences College, Qingdao Agriculture University, Qingdao 266109.
Liu
Jing
J
Hou
Li Xia
LX
Tang
Jing
J
Liu
Xin
X
D002118:Calcium / Q000378:metabolism*; D005030:Ethylenes / Q000494:pharmacology*; D050901:Nitrate Reductase / Q000378:metabolism; D009569:Nitric Oxide / Q000502:physiology*; D054046:Plant Stomata / Q000187:drug effects* / Q000502:physiology; D019810:Sodium Azide / Q000032:analysis / Q000506:poisoning; D031307:Vicia faba / Q000166:cytology* / Q000502:physiology
D005030:Ethylenes; D009569:Nitric Oxide; C036216:ethylene; D019810:Sodium Azide; D050901:Nitrate Reductase; D002118:Calcium