Studying the proliferation of human peripheral blood T lymphocytes in serum-free medium.
We compared the cultivation of human peripheral blood lymphocytes in serum-free medium Hybris-2 and RPMI 1640 medium with 10% fetal bovine serum in the presence of phytohemagglutinin and interleukin-2. The optimal concentration of phytohemagglutinin significantly differed in serum-free and serum-containing media (0.5 and 5 microg/ml, [corrected] respectively). Both mitogens were more potent in stimulating the proliferation of lymphocytes in serum-free medium than in serum-containing medium. Strong proliferation of CD3(+) and CD4(+) T lymphocytes was observed in both media. The dynamics of other markers was similar in serum-free and serum-containing media. However, significant differences were revealed between individual donors. Our results indicate that the developed serum-free medium may be used in lymphocyte cultivation for scientific, diagnostic, and therapeutic purposes.
Tabakov
V U
VU
Medical Genetic Research Center, Russian Academy of Medical Sciences, Moscow, Russia. biovitr@rambler.ru
Litvina
M M
MM
Schepkina
J V
JV
Jarilin
A A
AA
Chestkov
V V
VV
D017252:CD3 Complex / Q000276:immunology; D015704:CD4 Antigens / Q000276:immunology; D049109:Cell Proliferation; D002478:Cells, Cultured; D016895:Culture Media, Serum-Free; D006801:Humans; D007377:Interleukin-3 / Q000276:immunology; D010835:Phytohemagglutinins; D013601:T-Lymphocytes / Q000166:cytology* / Q000276:immunology
D017252:CD3 Complex; D015704:CD4 Antigens; D016895:Culture Media, Serum-Free; D007377:Interleukin-3; D010835:Phytohemagglutinins