In vivo fluorescence imaging with high-resolution microlenses.
Micro-optics are increasingly used for minimally invasive in vivo imaging, in miniaturized microscopes and in lab-on-a-chip devices. Owing to optical aberrations and lower numerical apertures, a main class of microlens, gradient refractive index lenses, has not achieved resolution comparable to conventional microscopy. Here we describe high-resolution microlenses, and illustrate two-photon imaging of dendritic spines on hippocampal neurons and dual-color nonlinear optical imaging of neuromuscular junctions in live mice.
Barretto
Robert P J
RP
James H Clark Center for Biomedical Engineering and Sciences, Stanford University, Stanford, California, USA.
Messerschmidt
Bernhard
B
Schnitzer
Mark J
MJ
D000818:Animals; D049229:Dendritic Spines / Q000378:metabolism / Q000648:ultrastructure; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D007909:Lenses; D051379:Mice; D008822:Mice, Transgenic; D036641:Microscopy, Fluorescence, Multiphoton / Q000295:instrumentation* / Q000379:methods; D009469:Neuromuscular Junction / Q000378:metabolism / Q000648:ultrastructure; D017966:Pyramidal Cells / Q000378:metabolism / Q000648:ultrastructure; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism
D011993:Recombinant Fusion Proteins; D049452:Green Fluorescent Proteins