title: PECAM-1 is involved in neutrophil transmigration across Histophilus somni treated bovine brain endothelial cells.
abstract: Histophilus somni (H. somni) is a gram-negative bacterial pathogen that causes respiratory, reproductive, and central nervous system disease in cattle. The hallmark of systemic H. somni infection is diffused vasculitis that can lead to an acute central nervous system disease known as thrombotic meningoencephalitis (TME). Because platelet endothelial cell adhesion molecule-1 (PECAM-1) and endothelial nitric oxide synthase (eNOS) play fundamental roles in maintaining homeostasis in blood vessels, we sought to determine if PECAM-1 and eNOS expression play a role in events related to the pathogenesis of TME. Our findings demonstrate that neutrophil transmigration across H. somni-treated TBBEC (SV-40 transformed bovine brain endothelial cell line) was reduced by treatment with anti-PECAM-1 antibodies. Confocal microscopy indicated that H. somni treatment leads to redistribution of PECAM-1 and eNOS on the surface of TBBEC. These findings suggest that PECAM-1 and eNOS may play a role in the early pathogenesis of TME.
authors.affiliation: Department of Pathobiological Sciences, University of Wisconsin-Madison, 53706, USA.
authors.forename: Raksha
authors.initials: R
authors.lastname: Tiwari
authors.forename: J
authors.initials: J
authors.lastname: Sullivan
authors.forename: C J
authors.initials: CJ
authors.lastname: Czuprynski
mesh_terms: D000818:Animals; D001921:Brain / Q000201:enzymology / Q000276:immunology / Q000382:microbiology; D002417:Cattle; D002418:Cattle Diseases / Q000201:enzymology / Q000276:immunology* / Q000382:microbiology; D002461:Cell Line, Transformed; D002478:Cells, Cultured; D002634:Chemotaxis, Leukocyte; D042783:Endothelial Cells / Q000201:enzymology / Q000276:immunology* / Q000382:microbiology; D044138:Haemophilus somnus / Q000276:immunology*; D008590:Meningoencephalitis / Q000201:enzymology / Q000276:immunology / Q000382:microbiology / Q000662:veterinary*; D009504:Neutrophils / Q000201:enzymology / Q000276:immunology* / Q000382:microbiology; D052250:Nitric Oxide Synthase Type III / Q000235:genetics / Q000276:immunology; D019408:Platelet Endothelial Cell Adhesion Molecule-1 / Q000235:genetics / Q000276:immunology*
chemical_list: D019408:Platelet Endothelial Cell Adhesion Molecule-1; D052250:Nitric Oxide Synthase Type III