title: Molecular genotyping of microbes by multilocus PCR and mass spectrometry: a new tool for hospital infection control and public health surveillance.
abstract: We describe a new technology for the molecular genotyping of microbes using a platform known commercially as the Ibis T5000. The technology couples multilocus polymerase chain reaction (PCR) to electrospray ionization/mass spectrometry (PCR/ESI-MS) and was developed to provide rapid, high-throughput, and precise digital analysis of either isolated colonies or original patient specimens on a platform suitable for use in hospital or reference diagnostic laboratories or public health settings. The PCR/ESI-MS method measures digital molecular signatures from microbes, enabling real-time epidemiological surveillance and outbreak investigation. This technology will facilitate understanding of the pathways by which infectious organisms spread and will enable appropriate interventions on a time frame not previously achievable.
authors.affiliation: Ibis Biosciences, 1896 Rutherford Rd., Carlsbad, CA, USA.
authors.forename: David J
authors.initials: DJ
authors.lastname: Ecker
authors.forename: Christian
authors.initials: C
authors.lastname: Massire
authors.forename: Lawrence B
authors.initials: LB
authors.lastname: Blyn
authors.forename: Steven A
authors.initials: SA
authors.lastname: Hofstadler
authors.forename: James C
authors.initials: JC
authors.lastname: Hannis
authors.forename: Mark W
authors.initials: MW
authors.lastname: Eshoo
authors.forename: Thomas A
authors.initials: TA
authors.lastname: Hall
authors.forename: Rangarajan
authors.initials: R
authors.lastname: Sampath
mesh_terms: D000151:Acinetobacter Infections / Q000453:epidemiology / Q000382:microbiology; D040981:Acinetobacter baumannii / Q000235:genetics / Q000302:isolation & purification; D003428:Cross Infection / Q000175:diagnosis / Q000382:microbiology / Q000517:prevention & control*; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification; D004279:DNA, Viral / Q000235:genetics / Q000302:isolation & purification; D004196:Disease Outbreaks; D005798:Genes, Bacterial; D005827:Genetics, Microbial / Q000379:methods*; D005838:Genotype; D006801:Humans; D009980:Influenza A virus / Q000235:genetics / Q000302:isolation & purification; D017720:Molecular Epidemiology / Q000379:methods*; D016133:Polymerase Chain Reaction / Q000379:methods; D020641:Polymorphism, Single Nucleotide; D011159:Population Surveillance / Q000379:methods*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D013290:Streptococcal Infections / Q000453:epidemiology / Q000382:microbiology; D013297:Streptococcus pyogenes / Q000235:genetics / Q000302:isolation & purification
chemical_list: D004269:DNA, Bacterial; D004279:DNA, Viral