Bacteriolytic activity of the alternative pathway of complement differs kinetically from the classical pathway.
The interaction between bacterial cells and activated complement components as a kinetic biological event is described. The bacteriolytic activity of complement in human and fish serum was assayed by measuring the decrease of bioluminescence of Escherichia coli transformed with lux genes. From the kinetic curves, the bacteriolytic CB(50)- and AB(50)-units were derived at any desired time point. It was observed that these values were irregular but decreased as a function of incubation time, and reached equal values during prolonged incubation, suggesting that the difference between the classical and alternative pathway activity is kinetic. From the kinetic curves, entirely new parameters could be derived: rate of the activation phase, rate of killing by the lytic phase and rate of killing by the entire pathway in undiluted serum. The rates of human and fish classical pathway were about five and two times higher than those of the alternative pathway respectively.
Kilpi
Maaria K
MK
Department of Biochemistry and Food Chemistry, University of Turku, 20014 Turku, Finland. maakil@utu.fi
Atosuo
Janne T
JT
Lilius
Esa-Matti E
EM
D000818:Animals; D001433:Bacteriolysis / Q000276:immunology*; D003167:Complement Activation / Q000276:immunology; D003170:Complement Pathway, Alternative / Q000276:immunology*; D003165:Complement System Proteins / Q000276:immunology*; D004926:Escherichia coli / Q000276:immunology; D029941:Fish Proteins / Q000276:immunology; D005453:Fluorescence; D006801:Humans; D007700:Kinetics; D049449:Luminescence; D008163:Luminescent Measurements / Q000379:methods; D017686:Oncorhynchus mykiss / Q000097:blood / Q000276:immunology; D044967:Serum / Q000276:immunology*
D029941:Fish Proteins; D003165:Complement System Proteins