RGD-modified acellular bovine pericardium as a bioprosthetic scaffold for tissue engineering.
Acellular biological tissues, including bovine pericardia (BP), have been proposed as natural biomaterials for tissue engineering. However, small pore size, low porosity and lack of extra cellular matrix (ECM) after native cell extraction directly restrict the seed cell adhesion, migration and proliferation and which is a vital problem for ABP's application in the tissue engineered heart valve (TEHV). In the present study, we treated acellular BP with acetic acid, which increased the scaffold pore size and porosity and conjugated RGD polypeptides to ABP scaffolds. After 10 days of culture in vitro, the human mesenchymal stem cells (hMSCs) attached the best and proliferated the fastest on RGD-modified acellular scaffolds, and the cell has grown deep into the scaffold. In contrast, a low density of cells attached to the unmodified scaffolds, with few infiltrating into the acellular tissues. These findings support the potential use of modified acellular BP as a scaffold for tissue engineered heart valves.
Dong
Xiaochao
X
Institute of Cardiovascular Surgery, Xijing Hospital, The Fourth Military Medical University, 17 Changle Western Road, Xi'an, Shaanxi Province 710032, People's Republic of China.
Wei
Xufeng
X
Yi
Wei
W
Gu
Chunhu
C
Kang
Xiaojun
X
Liu
Yang
Y
Li
Qiang
Q
Yi
Dinghua
D
D019342:Acetic Acid / Q000737:chemistry; D000818:Animals; D002417:Cattle; D018929:Cell Culture Techniques / Q000379:methods; D002454:Cell Differentiation; D049109:Cell Proliferation; D005109:Extracellular Matrix / Q000378:metabolism; D006350:Heart Valve Prosthesis; D006801:Humans; D008422:Materials Testing; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D009842:Oligopeptides / Q000737:chemistry*; D010496:Pericardium / Q000378:metabolism*; D016062:Porosity; D013314:Stress, Mechanical; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry*
D009842:Oligopeptides; C047981:arginyl-glycyl-aspartic acid; D019342:Acetic Acid