title: Agouti C57BL/6N embryonic stem cells for mouse genetic resources.
abstract: We report the characterization of a highly germline competent C57BL/6N mouse embryonic stem cell line, JM8. To simplify breeding schemes, the dominant agouti coat color gene was restored in JM8 cells by targeted repair of the C57BL/6 nonagouti mutation. These cells provide a robust foundation for large-scale mouse knockout programs that aim to provide a public resource of targeted mutations in the C57BL/6 genetic background.
authors.affiliation: Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Cambridge, UK.
authors.forename: Stephen J
authors.initials: SJ
authors.lastname: Pettitt
authors.forename: Qi
authors.initials: Q
authors.lastname: Liang
authors.forename: Xin Y
authors.initials: XY
authors.lastname: Rairdan
authors.forename: Jennifer L
authors.initials: JL
authors.lastname: Moran
authors.forename: Haydn M
authors.initials: HM
authors.lastname: Prosser
authors.forename: David R
authors.initials: DR
authors.lastname: Beier
authors.forename: Kent C
authors.initials: KC
authors.lastname: Lloyd
authors.forename: Allan
authors.initials: A
authors.lastname: Bradley
authors.forename: William C
authors.initials: WC
authors.lastname: Skarnes
mesh_terms: D054366:Agouti Signaling Protein / Q000235:genetics*; D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D003433:Crosses, Genetic; D017931:DNA Primers / Q000235:genetics; D053595:Embryonic Stem Cells / Q000166:cytology* / Q000378:metabolism*; D005260:Female; D018390:Gene Targeting; D005821:Genetic Techniques; D018095:Germ-Line Mutation; D006200:Hair Color / Q000235:genetics; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL / Q000145:classification / Q000235:genetics*; D018345:Mice, Knockout; D008817:Mice, Mutant Strains; D008822:Mice, Transgenic; D020641:Polymorphism, Single Nucleotide; D011247:Pregnancy; D018183:Transplantation Chimera / Q000235:genetics
chemical_list: D054366:Agouti Signaling Protein; D017931:DNA Primers; C515183:a protein, mouse