Production of heterologous proteins using the fission-yeast (Schizosaccharomyces pombe) expression system.
The fission yeast Schizosaccharomyces pombe is a particularly useful model for studying the function and regulation of genes from higher eukaryotes. The genome of Sc. pombe has been sequenced, and DNA microarray, proteome and transcriptome analyses have been carried out. Among the well-characterized yeast species, Sc. pombe is considered an attractive host for the production of heterologous proteins. Expression vectors for high-level expression in Sc. pombe have been developed and many foreign proteins have been successfully expressed. However, further improvements in the protein-expressing host systems are still required for the production of heterologous proteins involved in post-translational modification, metabolism and intracellular trafficking. This minireview focuses on recent advances in heterologous protein production by use of engineered fission-yeast strains.
Takegawa
Kaoru
K
Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University, Hakozaki 6-10-1, Fukuoka 812-8581, Japan. takegawa@agr.kyushu-u.ac.jp
Tohda
Hideki
H
Sasaki
Mayumi
M
Idiris
Alimjan
A
Ohashi
Takao
T
Mukaiyama
Hiroyuki
H
Giga-Hama
Yuko
Y
Kumagai
Hiromichi
H
D005818:Genetic Engineering; D005822:Genetic Vectors / Q000235:genetics; D020411:Oligonucleotide Array Sequence Analysis; D016133:Polymerase Chain Reaction; D011994:Recombinant Proteins / Q000096:biosynthesis* / Q000235:genetics*; D012568:Schizosaccharomyces / Q000235:genetics* / Q000378:metabolism
D011994:Recombinant Proteins