Determination of phenylalanine in blood by high-performance anion-exchange chromatography-pulsed amperometric detection to diagnose phenylketonuria.
We have developed a high-performance anion-exchange chromatography with pulsed amperometric detection method for the detection of phenylalanine (Phe) and diagnosis of phenylketonuria (PKU). Sample pretreatment steps were simplified without derivatization. The analyte was separated within 5 min. The detection limit (S/N=3) for Phe was 50 pg. Linear dynamic range was 1.23-14.43 mg/dL (r(2)=0.9999) for a dried blood spot. The mean recoveries of Phe for intra- and inter-day assays were found to be 96.87-104.16%. This method clearly differentiated PKU-positive groups from normal groups, and proved to be a practical procedure for rapid screening and follow-up monitoring of PKU.
Jeong
Ji-Seon
JS
Department of Oriental pharmaceutical sciences and Kyung Hee East-West Pharmaceutical Research Institute, College of Pharmacy, Kyung Hee University, Dongdaemoon-gu, Seoul 130-701, South Korea.
Sim
Hee-Jung
HJ
Lee
Yong-Moon
YM
Yoon
Hye-Ran
HR
Lee
Dong Hwan
DH
Hong
Seon-Pyo
SP
D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D002852:Chromatography, Ion Exchange / Q000379:methods*; D006801:Humans; D007231:Infant, Newborn; D015997:Neonatal Screening / Q000379:methods; D010649:Phenylalanine / Q000097:blood*; D010661:Phenylketonurias / Q000175:diagnosis*; D012680:Sensitivity and Specificity
D010649:Phenylalanine