Does incorporation of gene for green fluorescent protein in BP6 fibrosarcoma tumor cells depress their intraperitoneal growth in rats? (In honour of Nobel Prize laureates 2008--Osamu Shimomura, Martin Chalfie, Roger Y. Tsien).
This manuscript was in honour of Nobel Prize in chemistry "for the discovery and development of the green fluorescent protein, GFP" to Osamu Shimomura, Martin Chalfie, and Roger Y. Tsien, simultaneously a brief information about experience with GFP in experimental tumorigenesis used this study is also presented. The experimental data have showed that BP6 cells incorporated with GFP gene have had smaller ability to induce both experimental intraperitoneal and subcutaneous tumor process. It was anticipated that incorporation of GFP gene might change physiological properties of cytoskeleton and worsen adhesive characteristics of tumor cells. It was also supposed that aftertime GFP will enable to monitor proliferation of cells not only within experimental work, but also in human medicine. GFP could help (supposedly) as reporter of proliferation, but also can serve as "target" for guide of tumorigenesis inhibiting substances. These ideas which are consequences of our experiments we append as congratulation to Nobel Prize in chemistry of the 2008 (Fig. 2, Ref. 44). Full Text (Free, PDF) www.bmj.sk.
Valaskova
Z
Z
Institute of Pathophysiology, Faculty of Medicine, Comenius University, Bratislava, Slovakia. zuzana.valaskova@fmed.uniba.sk
Lackovicova
L
L
Vrabcova
M
M
Bizik
J
J
Perzelova
A
A
Macikova
I
I
Danihel
L
L
Kinova
S
S
Buckingham
T
T
Hulin
I
I
D000818:Animals; D045744:Cell Line, Tumor / Q000473:pathology / Q000502:physiology; D005260:Female; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000502:physiology*; D008297:Male; D009368:Neoplasm Transplantation; D010534:Peritoneal Neoplasms / Q000503:physiopathology*; D051381:Rats; D017208:Rats, Wistar; D014162:Transfection
D049452:Green Fluorescent Proteins