Global and gene-specific promoter methylation changes are related to anti-B[a]PDE-DNA adduct levels and influence micronuclei levels in polycyclic aromatic hydrocarbon-exposed individuals.
We investigated the effect of chronic exposure to polycyclic aromatic hydrocarbons (PAHs) on DNA methylation states (percentage of methylated cytosines (%mC)) in Polish male nonsmoking coke-oven workers and matched controls. Methylation states of gene-specific promoters (p53, p16, HIC1 and IL-6) and of Alu and LINE-1 repetitive elements, as surrogate measures of global methylation, were quantified by pyrosequencing in peripheral blood lymphocytes (PBLs). DNA methylation was evaluated in relation to PAH exposure, assessed by urinary 1-pyrenol and anti-benzo[a]pyrene diolepoxide (anti-B[a]PDE)-DNA adduct levels, a critical genetic damage from B[a]P. We also evaluated whether PAH-induced DNA methylation states were in turn associated with micronuclei in PBLs, an indicator of chromosomal instability.
Pavanello
Sofia
S
Occupational Health Section, Department of Environmental Medicine and Public Health, University of Padova, Padova, Italy. sofia.pavanello@unipd.it
Bollati
Valentina
V
Pesatori
Angela Cecilia
AC
Kapka
Lucyna
L
Bolognesi
Claudia
C
Bertazzi
Pier Alberto
PA
Baccarelli
Andrea
A
D000328:Adult; D001483:Base Sequence; D015415:Biomarkers / Q000097:blood / Q000652:urine; D016022:Case-Control Studies; D003077:Coke; D018736:DNA Adducts / Q000097:blood*; D019175:DNA Methylation; D016158:Genes, p53; D006801:Humans; D019570:Inhalation Exposure; D051741:Kruppel-Like Transcription Factors; D016014:Linear Models; D008297:Male; D048629:Micronuclei, Chromosome-Defective; D008875:Middle Aged; D008969:Molecular Sequence Data; D016273:Occupational Exposure / Q000009:adverse effects*; D011044:Poland; D011084:Polycyclic Aromatic Hydrocarbons / Q000633:toxicity*; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic; D017422:Sequence Analysis, DNA
D015415:Biomarkers; D003077:Coke; D018736:DNA Adducts; C499808:HIC1 protein, human; D051741:Kruppel-Like Transcription Factors; D011084:Polycyclic Aromatic Hydrocarbons