title: Cyclic stretch upregulates SDF-1alpha/CXCR4 axis in human saphenous vein smooth muscle cells.
abstract: Cyclic stretch (CS) mediates different cellular functions in vascular smooth muscle cells and involves in neointimal hyperplasia and subsequent atherosclerosis of vein grafts. Here, we investigated whether CS can modulate stromal cell-derived factor-1alpha (SDF-1alpha)/CXCR4 axis in human saphenous vein smooth muscle cells. We found CS induced the upregulation of SDF-1alpha and CXCR4 in human saphenous vein smooth muscle cells in vitro, which was dependent on PI3K/Akt/mTOR pathway. Furthermore, CS augmented human saphenous vein smooth muscle migration and focal adhesion kinase (FAK) activation by PI3K/Akt/mTOR pathway. Interestingly, the upregulation of SDF-1alpha/CXCR4 axis was instrumental in CS-induced saphenous vein smooth muscle cell migration and FAK activation, as showed by AMD3100, an inhibitor of SDF-1alpha/CXCR4 axis, partially but significantly blocked the CS-induced cellular effects. Thus, those data suggested SDF-1alpha/CXCR4 axis involves in CS-mediated cellular functions in human saphenous vein smooth muscle cells.
authors.affiliation: Department of Cardiology, Xijing Hospital, Fourth Military Medical University, Xi'an, China.
authors.forename: Fei
authors.initials: F
authors.lastname: Li
authors.forename: Wen-yi
authors.initials: WY
authors.lastname: Guo
authors.forename: Wei-jie
authors.initials: WJ
authors.lastname: Li
authors.forename: Dian-xin
authors.initials: DX
authors.lastname: Zhang
authors.forename: An-lin
authors.initials: AL
authors.lastname: Lv
authors.forename: Rong-hua
authors.initials: RH
authors.lastname: Luan
authors.forename: Bing
authors.initials: B
authors.lastname: Liu
authors.forename: Hai-chang
authors.initials: HC
authors.lastname: Wang
mesh_terms: D002465:Cell Movement; D054377:Chemokine CXCL12 / Q000096:biosynthesis* / Q000235:genetics; D006801:Humans; D040542:Mechanotransduction, Cellular; D009131:Muscle, Smooth, Vascular / Q000378:metabolism* / Q000502:physiology; D032389:Myocytes, Smooth Muscle / Q000378:metabolism* / Q000502:physiology; D019718:Receptors, CXCR4 / Q000096:biosynthesis* / Q000235:genetics; D012501:Saphenous Vein / Q000166:cytology / Q000378:metabolism* / Q000502:physiology; D013718:Tensile Strength; D015854:Up-Regulation
chemical_list: D054377:Chemokine CXCL12; D019718:Receptors, CXCR4