title: Prevalence of Burkholderia pseudomallei in Guangxi, China.
abstract: Melioidosis, an infectious disease caused by the Gram-negative bacterium Burkholderia pseudomallei, is now recognized as an important public health problem in Southeast Asia and tropical northern Australia. Although B. pseudomallei has been detected in various water and soil samples in southeast China, the enviromental distribution of B. pseudomallei in China is unclear. In the winter months of 2007, 154 and 130 soil and water samples, respectively, were collected from several locations in Guangxi, China. The samples were screened for B. pseudomallei by bacterial culture and identification and confirmed by PCR for species-specific 16S rDNA and flagellin genes. B. pseudomallei was detected in 8.4% of the soil samples but in none of the water samples. All positive samples were confined to a single low-lying region from rice paddy fields. Counts of B. pseudomallei ranged from 23 to 521 c.f.u./g soil. This is the first geographical distribution survey of B. pseudomallei in soil in Guangxi, China, and the data are of importance for further evaluating the impact of this pathogen on melioidosis in this region.
authors.affiliation: State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, China.
authors.forename: G
authors.initials: G
authors.lastname: Ma
authors.forename: D
authors.initials: D
authors.lastname: Zheng
authors.forename: Q
authors.initials: Q
authors.lastname: Cai
authors.forename: Z
authors.initials: Z
authors.lastname: Yuan
mesh_terms: D016957:Burkholderia pseudomallei / Q000235:genetics / Q000302:isolation & purification*; D002681:China; D006801:Humans; D016133:Polymerase Chain Reaction; D012988:Soil Microbiology; D014871:Water Microbiology