Improved isolation protocol for equine cord blood-derived mesenchymal stromal cells.
OBJECTIVE
A robust methodology for the isolation of cord blood-derived multipotent mesenchymal stromal cells (CB-MSCs) from fresh umbilical cord blood has not been reported in any species. The objective of this study was to improve the isolation procedure for equine CB-MSCs.


METHODS
Pre-culture separation of red and white blood cells was done using either PrepaCyte?-EQ medium or Ficoll-Paque? PREMIUM density medium. Regular FBS and MSC-qualified FBS were compared for their ability to support the establishment of putative primary MSC colonies.


CONCLUSIONS
Our results indicate that PrepaCyte-EQ medium is superior to Ficoll-Paque PREMIUM density medium for the isolation of putative equine CB MSC and that MSC-qualified FBS may improve the isolation efficiency.
Koch
Thomas G
TG
Department of Biomedical Sciences, University of Guelph, Ontario, Guelph, Canada. tkoch@uoguelph.ca
Thomsen
Preben D
PD
Betts
Dean H
DH
D050156:Adipogenesis; D000818:Animals; D002469:Cell Separation / Q000379:methods*; D020219:Chondrogenesis; D003114:Colony-Forming Units Assay; D005312:Fetal Blood / Q000166:cytology*; D006736:Horses; D007963:Leukocytes, Mononuclear / Q000166:cytology; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D010012:Osteogenesis; D017154:Stromal Cells / Q000166:cytology*