title: Continuous uninterrupted single medium culture without medium renewal versus sequential media culture: a sibling embryo study.
abstract: Eight hundred ninety-three sibling embryos from 80 IVF cycles were cultured side by side in either: 1) a single medium continuously, without medium renewal on day 3; or 2) sequential media. There were no significant differences between the two culture media systems regarding embryo quality or the proportion of embryos selected for transfer on day 3 from either media; however, for day 5 embryo transfer, a greater number of blastocysts were available, and were selected for transfer, from the continuous single medium culture compared with sequential media culture.
authors.affiliation: Center for Reproductive Medicine of New Mexico, Albuquerque, New Mexico 87106, USA. mleroyreed@yahoo.com
authors.forename: Michael L
authors.initials: ML
authors.lastname: Reed
authors.forename: Amanda
authors.initials: A
authors.lastname: Hamic
authors.forename: Douglas J
authors.initials: DJ
authors.lastname: Thompson
authors.forename: Charles L
authors.initials: CL
authors.lastname: Caperton
mesh_terms: D000328:Adult; D003470:Culture Media / Q000737:chemistry / Q000494:pharmacology*; D046149:Embryo Culture Techniques / Q000379:methods*; D004624:Embryo Transfer / Q000379:methods; D004622:Embryo, Mammalian / Q000187:drug effects*; D047108:Embryonic Development / Q000187:drug effects; D005260:Female; D006801:Humans; D008297:Male; D016555:Matched-Pair Analysis; D011247:Pregnancy; D018873:Pregnancy Rate; D035781:Siblings; D020554:Sperm Injections, Intracytoplasmic / Q000379:methods
chemical_list: D003470:Culture Media