title: [Replication of HCV in bone marrow of patients with haematological disorders].
abstract: The aim of the study was to evaluate the presence of HCV replication in bone marrow cells derived from patients displaying hematological disorders. We analysed serum, peripheral blood mononuclear cells (PBMC) and bone marrow samples obtained from 27 patients displaying the following dysfunctions: lymphoma, trombocytopenia, haemophilia, pancytopenia and acute myeloid leukemia (AML). The presence of HCV-RNA in samples was detected by RT-PCR. All the serum samples were HCV-RNA positive as well as 9 out of 27 (33%) PBMC and 17 out of 27 (63%) of bone marrow samples. Independently to the disorder type, the co-presence of HCV-RNA in serum and bone marrow with the simultaneous absence of the viral genetic material in PBMC was detected in 5 (18.5%) of patients. This result suggests that bone marrow is a site of active viral replication. To check whether a viral replication generates any mutations, an SSCP analysis of the 5'UTR viral region was performed. The difference in the viral sequence derived from serum, PBMC and bone marrow was detected in one case. This result may indicate the occurrence of mutation process during the viral replication in bone marrow. An immunohistochemical analysis of bone marrow smears showed the presence of HCV antigens.
  
  
  CONCLUSIONS
  bone marrow cells of patients displaying hematological disorders represent a putative site of extrahepatic HCV replication.
authors.affiliation: Zakład Immunopatologii Chorób Zakaźnych i Pasozytniczych Warszawski Uniwersytet Medyczny. agnieszka.pawelczyk@wum.edu.pl
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Pawełczyk
authors.forename: Marta
authors.initials: M
authors.lastname: Polańska
authors.forename: Elzbieta
authors.initials: E
authors.lastname: Kisiel
authors.forename: Marcin
authors.initials: M
authors.lastname: Chmielewski
authors.forename: Iwona
authors.initials: I
authors.lastname: Bukowska
authors.forename: Maria
authors.initials: M
authors.lastname: Fic
authors.forename: Marek
authors.initials: M
authors.lastname: Radkowski
mesh_terms: D001853:Bone Marrow / Q000821:virology*; D001854:Bone Marrow Cells / Q000821:virology; D005260:Female; D006402:Hematologic Diseases / Q000821:virology*; D006467:Hemophilia A / Q000821:virology; D016174:Hepacivirus / Q000502:physiology*; D006526:Hepatitis C / Q000150:complications / Q000175:diagnosis*; D006801:Humans; D015470:Leukemia, Myeloid, Acute / Q000821:virology; D007963:Leukocytes, Mononuclear / Q000821:virology*; D008223:Lymphoma / Q000821:virology; D008297:Male; D010198:Pancytopenia / Q000821:virology; D011044:Poland; D012367:RNA, Viral / Q000032:analysis; D014779:Virus Replication
chemical_list: D012367:RNA, Viral