title: Insulin-binding canavalin is present in Canavalia ensiformis seed coat.
abstract: An insulin-binding protein was isolated from Canavalia ensiformis seed coat, by using an insulin-Sepharose 4B affinity chromatography, and the protein was identified as canavalin (Canavalia 7S globulin) by mass spectrometry analysis. The major novelty of these data is the acidic nature of this globulin insulin-binding, in contrast to the basic Bg-like insulin-binding proteins so far reported in plants.
authors.affiliation: Laboratório de Química e Função de Proteínas e Peptídeos, Centro de Biociôncias e Biotecnologia, Universidade Estadual do Norte Fluminense, 28013-602, Campos dos Goytacazes, RJ, Brazil.
authors.forename: Elane da Silva
authors.initials: Eda S
authors.lastname: Ribeiro
authors.forename: Adriana Ferreira
authors.initials: AF
authors.lastname: Uchôa
authors.forename: Leonardo Gomes
authors.initials: LG
authors.lastname: da Silva
authors.forename: Daniela Gois
authors.initials: DG
authors.lastname: Beghini
authors.forename: Andre Teixeira da Silva
authors.initials: AT
authors.lastname: Ferreira
authors.forename: Jonas
authors.initials: J
authors.lastname: Perales
authors.forename: Tânia
authors.initials: T
authors.lastname: Jacinto
authors.forename: Katia Valevski Sales
authors.initials: KV
authors.lastname: Fernandes
authors.forename: José
authors.initials: J
authors.lastname: Xavier-Filho
authors.forename: Antonia Elenir Amancio
authors.initials: AE
authors.lastname: Oliveira
mesh_terms: D032449:Canavalia / Q000737:chemistry*; D002352:Carrier Proteins; D002846:Chromatography, Affinity; D005916:Globulins / Q000737:chemistry; D007328:Insulin / Q000737:chemistry*; D010940:Plant Proteins / Q000737:chemistry*; D012639:Seeds / Q000737:chemistry
chemical_list: D002352:Carrier Proteins; D005916:Globulins; D007328:Insulin; D010940:Plant Proteins; C006882:canavalin protein, Canavalia ensiformis