title: Layer-by-Layer-Coated Gelatin Nanoparticles as a Vehicle for Delivery of Natural Polyphenols.
abstract: Natural polyphenols with previously demonstrated anticancer potential, epigallocatechin gallate (EGCG), tannic acid, curcumin, and theaflavin, were encased into gelatin-based 200 nm nanoparticles consisting of a soft gel-like interior with or without a surrounding LbL shell of polyelectrolytes (polystyrene sulfonate/polyallylamine hydrochloride, polyglutamic acid/poly-l-lysine, dextran sulfate/protamine sulfate, carboxymethyl cellulose/gelatin, type A) assembled using the layer-by-layer technique. The characteristics of polyphenol loading and factors affecting their release from the nanocapsules were investigated. Nanoparticle-encapsulated EGCG retained its biological activity and blocked hepatocyte growth factor (HGF)-induced intracellular signaling in the breast cancer cell line MBA-MD-231 as potently as free EGCG.
authors.affiliation: Institute for Micromanufacturing and Biomedical Engineering Program, Louisiana Tech University, Ruston, Louisiana 71272.
authors.forename: Tatsiana G
authors.initials: TG
authors.lastname: Shutava
authors.forename: Shantanu S
authors.initials: SS
authors.lastname: Balkundi
authors.forename: Pranitha
authors.initials: P
authors.lastname: Vangala
authors.forename: Joshua J
authors.initials: JJ
authors.lastname: Steffan
authors.forename: Rebecca L
authors.initials: RL
authors.lastname: Bigelow
authors.forename: James A
authors.initials: JA
authors.lastname: Cardelli
authors.forename: D Patrick
authors.initials: DP
authors.lastname: O'Neal
authors.forename: Yuri M
authors.initials: YM
authors.lastname: Lvov