Effects of ethanol and lipopolysaccharide on the sphingomyelin cycle in rat hepatocytes.
Lipopolysaccharide toxin added to primary hepatocyte culture slightly modified the basal concentrations of (3)H-serine-labeled sphingomyelin, sphingosine, and ceramide. Ethanol reduced the levels of sphingomyelin and sphingosine by 20-25 and 15-20%, respectively, but increased ceramide content by 7-17%. Tumor necrosis factor reduced the concentrations of sphingomyelin and sphingosine, but did not modify the content of ceramide. Combined treatment with lipopolysaccharide toxin and ethanol potentiated the effect of alcohol.
Viktorov
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AV
Laboratory of Molecular Mechanisms of Infections, Institute of Epidemiology, Moscow, Russia. viktorov_av@yahoo.com
Yurkiv
V A
VA
D000818:Animals; D002478:Cells, Cultured; D002492:Central Nervous System Depressants / Q000633:toxicity*; D002518:Ceramides / Q000378:metabolism; D002855:Chromatography, Thin Layer; D000431:Ethanol / Q000633:toxicity*; D022781:Hepatocytes / Q000187:drug effects* / Q000378:metabolism*; D008070:Lipopolysaccharides / Q000633:toxicity*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013109:Sphingomyelins / Q000378:metabolism*; D013110:Sphingosine / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology
D002492:Central Nervous System Depressants; D002518:Ceramides; D008070:Lipopolysaccharides; D013109:Sphingomyelins; D014409:Tumor Necrosis Factor-alpha; D000431:Ethanol; D013110:Sphingosine