title: Chemoradiation interactions under reduced oxygen conditions: Cellular characteristics of an in vitro model.
abstract: Hypoxic tumour regions often contain viable cells that are more resistant to chemotherapy and/or radiotherapy, making it of key importance to analyse new combination treatments under both normoxic and hypoxic conditions. In this study, the impact of moderate hypoxia and anoxia on cellular characteristics was investigated in isogenic A549 cells differing in p53 status. VEGF expression, doubling time, cell cycle distribution, induction of apoptosis and p53 protein expression were evaluated. Radiation survival curves yielded an oxygen enhancement ratio of 1.16-1.67. In conclusion, an in vitro hypoxia model that will be highly useful to analyse chemoradiation interactions is presented.
authors.affiliation: Laboratory of Cancer Research and Clinical Oncology, Department of Medical Oncology, University of Antwerp, 2610 Wilrijk, Belgium. An.Wouters@ua.ac.be
authors.forename: An
authors.initials: A
authors.lastname: Wouters
authors.forename: Bea
authors.initials: B
authors.lastname: Pauwels
authors.forename: Hilde A J
authors.initials: HA
authors.lastname: Lambrechts
authors.forename: Greet G O
authors.initials: GG
authors.lastname: Pattyn
authors.forename: Johan
authors.initials: J
authors.lastname: Ides
authors.forename: Marc
authors.initials: M
authors.lastname: Baay
authors.forename: Paul
authors.initials: P
authors.lastname: Meijnders
authors.forename: Sylvia
authors.initials: S
authors.lastname: Dewilde
authors.forename: Jan B
authors.initials: JB
authors.lastname: Vermorken
authors.forename: Filip
authors.initials: F
authors.lastname: Lardon
mesh_terms: D017209:Apoptosis / Q000187:drug effects* / Q000528:radiation effects; D015153:Blotting, Western; D002453:Cell Cycle / Q000187:drug effects / Q000528:radiation effects; D015687:Cell Hypoxia; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects / Q000528:radiation effects; D002470:Cell Survival / Q000187:drug effects / Q000528:radiation effects; D006801:Humans; D010100:Oxygen / Q000378:metabolism / Q000494:pharmacology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013997:Time Factors; D016159:Tumor Suppressor Protein p53 / Q000235:genetics / Q000378:metabolism*; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics / Q000378:metabolism*
chemical_list: D016159:Tumor Suppressor Protein p53; D042461:Vascular Endothelial Growth Factor A; D010100:Oxygen