Inhibition of cell proliferation, migration and invasion by DNAzyme targeting MMP-9 in A549 cells.
Matrix metalloproteinases (MMPs) have been regarded as major critical molecules assisting tumor cells during angiogenesis and metastasis. Enhanced expression of matrix metalloproteinase-9 (MMP-9) is associated with human non-small cell lung cancer (NSCLC) invasion and metastasis. DNAzyme is a single-stranded DNA catalyst that can be engineered to bind to its complementary sequence in the target gene and cleave the mRNA. In this study, DNAzyme targeting MMP-9 was designed and synthesized. We found it strongly inhibited MMP-9 mRNA and protein expression in the NSCLC cell line A549. Inhibition of cell proliferation, adhesion, migration and invasion was also demonstrated. Thus, DNAzyme targeting MMP-9 may be a promising anti-invasion and anti-metastasis strategy for cancer gene therapy in NSCLC.
Yang
Lifang
L
Cancer Research Institute, Xiangya School of Medicine, Central South University, Hunan, P.R. China.
Zeng
Weizhong
W
Li
Dan
D
Zhou
Rui
R
D002448:Cell Adhesion; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D021881:DNA, Catalytic / Q000235:genetics / Q000378:metabolism*; D015536:Down-Regulation; D015971:Gene Expression Regulation, Enzymologic; D015972:Gene Expression Regulation, Neoplastic; D015316:Genetic Therapy; D006801:Humans; D008175:Lung Neoplasms / Q000201:enzymology* / Q000235:genetics / Q000473:pathology; D020780:Matrix Metalloproteinase 9 / Q000235:genetics / Q000378:metabolism*; D009361:Neoplasm Invasiveness; D012333:RNA, Messenger / Q000378:metabolism; D013997:Time Factors; D014162:Transfection
D021881:DNA, Catalytic; D012333:RNA, Messenger; D020780:Matrix Metalloproteinase 9