title: Assay conditions and validation of a new UV spectrophotometric method using microplates for the determination of polyphenol content.
abstract: A new spectrophotometric assay for the quantification of polyphenolic content has been validated. It is based on Prussian Blue method and adapted to microplate spectrophotometry. Prussian Blue reaction is critically dependent on reaction time, but microplate spectrophotometry permits exact measures of many samples at the same time. This new method is precise, reproducible, repeatable and exact.
authors.affiliation: Department of Pharmacy and Pharmaceutical Technology, School of Pharmacy, University of Navarra, Pamplona (Navarra), Spain. iuripue@yahoo.com
authors.forename: Iñigo Uriarte
authors.initials: IU
authors.lastname: Pueyo
authors.forename: María Isabel
authors.initials: MI
authors.lastname: Calvo
mesh_terms: D000975:Antioxidants / Q000032:analysis; D005419:Flavonoids / Q000032:analysis*; D010636:Phenols / Q000032:analysis*; D010936:Plant Extracts / Q000737:chemistry*; D010944:Plants / Q000737:chemistry*; D059808:Polyphenols; D011540:Prussian Blue Reaction; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet / Q000295:instrumentation / Q000379:methods*
chemical_list: D000975:Antioxidants; D005419:Flavonoids; D010636:Phenols; D010936:Plant Extracts; D059808:Polyphenols