miR-29b expression is associated with disease-free survival in patients with ovarian serous carcinoma.
Micro-RNAs are a group of small noncoding RNAs approximately 22 nucleotides in length. Recent work has shown differential expression of mature micro-RNAs in human cancers. We characterized the alteration in expression of miR-29b in ovarian serous carcinoma. miR-29b expression was analyzed using quantitative stem-loop reverse transcriptase polymerase chain reaction on a set of 50 formalin-fixed, paraffin-embedded ovarian serous carcinoma samples. Protein expression of p53, estrogen receptor, progesterone receptor, human epidermal growth factor receptor 2, Ki-67, and insulinlike growth factor 1 was quantified in the corresponding tissue microarray. The expression profile of miR-29b was correlated with clinicopathological and patient survival data. We provide definitive evidence that miR-29b is down-regulated in a significant proportion of ovarian serous carcinomas and is associated with specific clinicopathological features, most notably high miR-29b expression being associated with reduced disease-free survival.
Flavin
Richard
R
Department of Histopathology, Trinity College, Dublin, Ireland. flavinr@tcd.ie
Smyth
Paul
P
Barrett
Ciara
C
Russell
S
S
Wen
Hannah
H
Wei
Jianjun
J
Laios
Alex
A
O'Toole
Sharon
S
Ring
M
M
Denning
K
K
Li
J
J
Aherne
S
S
Sammarae
D
D
Aziz
N A
NA
Alhadi
A
A
Finn
Sephen P
SP
Loda
M
M
B
Sheppard
S
Sheils
Orla
O
O'Leary
John J
JJ
D018284:Cystadenocarcinoma, Serous / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D018572:Disease-Free Survival; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D035683:MicroRNAs / Q000096:biosynthesis* / Q000235:genetics; D010051:Ovarian Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D020133:Reverse Transcriptase Polymerase Chain Reaction
C532045:MIRN29a microRNA, human; D035683:MicroRNAs