Layer-by-Layer-Coated Gelatin Nanoparticles as a Vehicle for Delivery of Natural Polyphenols.
Natural polyphenols with previously demonstrated anticancer potential, epigallocatechin gallate (EGCG), tannic acid, curcumin, and theaflavin, were encased into gelatin-based 200 nm nanoparticles consisting of a soft gel-like interior with or without a surrounding LbL shell of polyelectrolytes (polystyrene sulfonate/polyallylamine hydrochloride, polyglutamic acid/poly-l-lysine, dextran sulfate/protamine sulfate, carboxymethyl cellulose/gelatin, type A) assembled using the layer-by-layer technique. The characteristics of polyphenol loading and factors affecting their release from the nanocapsules were investigated. Nanoparticle-encapsulated EGCG retained its biological activity and blocked hepatocyte growth factor (HGF)-induced intracellular signaling in the breast cancer cell line MBA-MD-231 as potently as free EGCG.
Shutava
Tatsiana G
TG
Institute for Micromanufacturing and Biomedical Engineering Program, Louisiana Tech University, Ruston, Louisiana 71272.
Balkundi
Shantanu S
SS
Vangala
Pranitha
P
Steffan
Joshua J
JJ
Bigelow
Rebecca L
RL
Cardelli
James A
JA
O'Neal
D Patrick
DP
Lvov
Yuri M
YM