title: Characterization and expression analysis of type I interferon in common carp Cyprinus carpio L.
abstract: The type I interferon (I-IFN) gene has recently been cloned and sequenced in the common carp species Cyprinus carpio L. Carp I-IFN cDNA is composed of 675 base pairs and is translated into a protein of 186 amino acid residues. The carp I-IFN encodes a predicted signal peptide of 23 amino acid residues and contains the I-IFN family signature His(140)-Trp(158). Analysis of the homology between carp I-IFN and other known I-IFN and type II interferon (II-IFN) family members has revealed significant similarities to grass carp I-IFN. Phylogenetic analysis demonstrated that carp I-IFN clusters with I-IFN in teleosts, away from the other II-IFN family members. In addition, the gene structure for carp I-IFN is composed of 5 exons and 4 introns, a composition that is similar to that of the teleost I-IFN gene. RT-PCR analysis did not reveal gene expression in un-stimulated tissues including intestine, liver, gill, head kidney, muscle, spleen, mid-kidney and skin. However, I-IFN expression levels increased following stimulation with imiquimod in the head kidney cells. Furthermore, recombinant carp I-IFN protein (mature form) produced via the cell-free protein synthesis system stimulated the expression of the interferon-inducible Mx gene in the head kidney cells.
authors.affiliation: University of Miyazaki, Japan.
authors.forename: Yoichi
authors.initials: Y
authors.lastname: Kitao
authors.forename: Tomoya
authors.initials: T
authors.lastname: Kono
authors.forename: Hiroki
authors.initials: H
authors.lastname: Korenaga
authors.forename: Toshiyuki
authors.initials: T
authors.lastname: Iizasa
authors.forename: Keisuke
authors.initials: K
authors.lastname: Nakamura
authors.forename: Ram
authors.initials: R
authors.lastname: Savan
authors.forename: Masahiro
authors.initials: M
authors.lastname: Sakai
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002347:Carps / Q000235:genetics*; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D029941:Fish Proteins / Q000235:genetics*; D020869:Gene Expression Profiling; D007370:Interferon Type I / Q000145:classification / Q000235:genetics*; D008969:Molecular Sequence Data; D010802:Phylogeny; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
chemical_list: D018076:DNA, Complementary; D029941:Fish Proteins; D007370:Interferon Type I