Detection and quantification of hepatitis C virus (HCV) by MultiCode-RTx real-time PCR targeting the HCV 3' untranslated region.
A prototype, real-time reverse-transcription PCR assay, based on MultiCode-RTx technology, quantifying hepatitis C virus (HCV) RNA by targeting the HCV 3' untranslated region demonstrated linearity over 7 logs, with a good correlation between the quantitative results of this assay and the results of two commercially available comparator assays for 466 clinical specimens comprising all six HCV genotypes.
Mulligan
Elizabeth K
EK
EraGen Biosciences, Inc., Madison, WI 53717, USA.
Germer
J J
JJ
Arens
Max Q
MQ
D'Amore
Krista L
KL
Di Bisceglie
Adrian
A
Ledeboer
Nathan A
NA
Moser
Michael J
MJ
Newman
Andrew C
AC
O'Guin
Andy K
AK
Olivo
Paul D
PD
Podzorski
Diane S
DS
Vaughan
Kimberly A
KA
Yao
Joseph D
JD
Elagin
Slava A
SA
Johnson
Scott C
SC
D020413:3' Untranslated Regions; D016174:Hepacivirus / Q000235:genetics / Q000302:isolation & purification*; D006526:Hepatitis C / Q000175:diagnosis* / Q000821:virology; D006801:Humans; D010949:Plasma / Q000821:virology; D012367:RNA, Viral / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D044967:Serum / Q000821:virology; D019562:Viral Load / Q000379:methods*
D020413:3' Untranslated Regions; D012367:RNA, Viral