title: Proteomic analysis of cell surface-associated proteins from probiotic Lactobacillus plantarum.
abstract: In the present study, we used a proteomic approach to identify surface-associated proteins from the probiotic bacterium Lactobacillus plantarum 299v. Proteins were extracted from the cell surface using a mild wash in phosphate buffer and analysed by sodium dodecyl sulphate-polyacrylamide gel electrophoresis. Gel bands were excised and in-gel digested with trypsin. The resulting peptides were analysed by capillary-LC-ESI-MS/MS. The peptide sequences were used for a database search and allowed identification of a total of 29 proteins, many of which could potentially be involved in the action of probiotics in the gastrointestinal tract. The results provide the basis for future studies on the molecular mechanisms of probiotics.
authors.affiliation: Danish Technological Institute, Kolding, Denmark. hans.christian.beck@teknologisk.dk
authors.forename: Hans Christian
authors.initials: HC
authors.lastname: Beck
authors.forename: Søren M
authors.initials: SM
authors.lastname: Madsen
authors.forename: Jacob
authors.initials: J
authors.lastname: Glenting
authors.forename: Jørgen
authors.initials: J
authors.lastname: Petersen
authors.forename: Hans
authors.initials: H
authors.lastname: Israelsen
authors.forename: Mette Rindom
authors.initials: MR
authors.lastname: Nørrelykke
authors.forename: Martin
authors.initials: M
authors.lastname: Antonsson
authors.forename: Anne Maria
authors.initials: AM
authors.lastname: Hansen
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D048191:Lactiplantibacillus plantarum / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D008565:Membrane Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D019936:Probiotics / Q000737:chemistry* / Q000378:metabolism; D040901:Proteomics
chemical_list: D001426:Bacterial Proteins; D008565:Membrane Proteins