The use of tannins to control Salmonella typhimurium infections in pigs.
The aim of this study was to determine whether a hydrolysable tannin extract of sweet chestnut wood (Globatan(®)) has an inhibitory effect on Salmonella Typhimurium survival both in vitro and in vivo in pigs. In a first experiment, the minimal inhibitory concentration of Globatan(®) on 57 Salmonella Typhimurium isolates was determined. For all isolates, an MIC of 160-320 μg/ml was found. The second in vitro study revealed that Salmonella growth was strongly reduced using Globatan(®) concentrations of 25-50 μg/ml and nearly completely inhibited at a concentration of 100 μg/ml Globatan(®). In an in vivo trial, two groups of six piglets, each group receiving feed with or without the addition of Globatan(®) (3 g/kg), were orally inoculated with 10(7) colony forming units of a Salmonella Typhimurium strain. Globatan(®) had no effect on faecal excretion of Salmonella, and no differences in colonization of the intestines and internal organs were demonstrated in pigs euthanized at 4 days post-inoculation. In conclusion, the hydrolysable tannin extract used in this study showed strong action against Salmonella Typhimurium in vitro but not in vivo.
Van Parys
A
A
Department of Pathology, Bacteriology and Avian Diseases, Faculty of Veterinary Medicine, University of Ghent, Merelbeke, Belgium. alexander.vanparys@ugent.be
Boyen
F
F
Dewulf
J
J
Haesebrouck
F
F
Pasmans
F
F
D000818:Animals; D015169:Colony Count, Microbial; D004305:Dose-Response Relationship, Drug; D005243:Feces / Q000382:microbiology; D047348:Hydrolyzable Tannins / Q000494:pharmacology*; D008826:Microbial Sensitivity Tests; D010936:Plant Extracts / Q000494:pharmacology; D012481:Salmonella Infections, Animal / Q000382:microbiology / Q000517:prevention & control*; D012486:Salmonella typhimurium / Q000187:drug effects* / Q000254:growth & development / Q000302:isolation & purification; D013552:Swine; D013553:Swine Diseases / Q000382:microbiology / Q000517:prevention & control*
D047348:Hydrolyzable Tannins; D010936:Plant Extracts