Probiotic fortified diet reduces bacterial colonization and translocation in a long-term neonatal rabbit model.
BACKGROUND
Probiotic fortified diet reduces bacterial colonization and translocation in a short-term neonatal rabbit model when continuously challenged with pathogen. The purpose of this study was to determine if live probiotic diet could remain effective at decreasing colonization/translocation of pathogens in a long-term neonatal rabbit model without ill effects of the probiotic outside the gastrointestinal (GI) tract.


METHODS
Rabbit pups were born via cesarean delivery 1 day preterm and assigned to 2 diets: a newly formulated diet (controls) vs the same diet fortified with the live probiotic Lactoccocus lactis. Enterobacter cloacae was added to both preparations before each feed. Pups were gavage fed twice daily, and weights were recorded. Rabbits were sacrificed on day 7, and organs were harvested and cultured for target organism growth.


RESULTS
The probiotic fortified diet resulted in a significant decrease in Enterobacter translocation to the liver and decreased colonization in the stomach and lungs. There was no evidence of Lactococccus translocation or colonization outside of the GI tract.


CONCLUSIONS
This probiotic fortified diet was effective at decreasing pathogenic bacteria colonization and translocation in a long-term neonatal model. The addition of L lactis to the diet resulted in appropriate growth without any colonization or translocation of the probiotic outside of the GI tract.
Copeland
Daniel R
DR
Pediatric Surgery, Arkansas Children's Hospital, Little Rock, AR 72202-3591, USA.
McVay
Marcene R
MR
Dassinger
Melvin S
MS
Jackson
Richard J
RJ
Smith
Samuel D
SD
D000818:Animals; D000831:Animals, Newborn; D018988:Bacterial Translocation; D004195:Disease Models, Animal; D016972:Enterobacter cloacae / Q000502:physiology*; D004756:Enterobacteriaceae Infections / Q000517:prevention & control*; D005527:Food, Fortified; D019936:Probiotics / Q000008:administration & dosage*; D011817:Rabbits