Identification of plasmid-mediated quinolone resistance qnr-like genes in Romanian clinical isolates of Escherichia coli and Klebsiella pneumoniae.
A collection of putative ESBL-producing Escherichia coli (119 isolates) and Klebsiella pneumoniae (122 isolates) originating from extraintestinal human specimens was screened for qnrA, qnrB, and qnrS-like genes by PCR. Seven K. pneumoniae isolates, which were resistant to ciprofloxacin, were detected as carrying qnrA1-like genes, while one K. pneumoniae and one E. coli isolate were positive for qnrS-like determinant. The latter isolates were susceptible to ciprofloxacin. This is the first study identifying qnr-like genes in our area. Further studies are needed to document the contribution of the plasmid mediated quinolone resistance to the increase in bacterial resistance to fluoroquinolones in Romania.
Usein
Codruţa-Romaniţa
CR
Molecular Microbiology Laboratory, National Institute of Research-Development for Microbiology and Immunology Cantacuzino.
Palade
Andi-Marian
AM
Tatu-Chiţoiu
Dorina
D
Ciontea
Simona
S
Ceciu
Stefania
S
Nica
Maria
M
Damian
Maria
M
D000890:Anti-Infective Agents / Q000494:pharmacology; D001426:Bacterial Proteins / Q000032:analysis / Q000235:genetics*; D002939:Ciprofloxacin / Q000494:pharmacology; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics; D024901:Drug Resistance, Multiple, Bacterial; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification; D004927:Escherichia coli Infections / Q000188:drug therapy / Q000235:genetics / Q000382:microbiology*; D029968:Escherichia coli Proteins / Q000032:analysis / Q000235:genetics*; D005798:Genes, Bacterial; D006801:Humans; D007710:Klebsiella Infections / Q000188:drug therapy / Q000235:genetics / Q000382:microbiology*; D007711:Klebsiella pneumoniae / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification; D010957:Plasmids / Q000032:analysis / Q000235:genetics*; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D012383:Romania
D000890:Anti-Infective Agents; D001426:Bacterial Proteins; D004269:DNA, Bacterial; D029968:Escherichia coli Proteins; C485384:Qnr protein, E coli; C485385:Qnr protein, Klebsiella pneumoniae; D002939:Ciprofloxacin