Myeloperoxidase concentrations in EDTA-plasma of healthy subjects are discordant with concentrations in heparin-plasma and serum.
OBJECTIVE
To examine the effect of blood anticoagulation type on the myeloperoxidase (MPO) concentration.


METHODS
MPO was measured in EDTA-plasma and matched heparin-plasma and serum samples collected from healthy volunteers.


RESULTS
MPO concentrations in heparin-plasma and serum were higher than in EDTA-plasma (both P<0.001). MPO in EDTA-plasma was not significantly correlated with MPO in either heparin-plasma or serum.


CONCLUSIONS
For MPO measurements, EDTA-plasma is the preferred specimen as it appears unaffected by ex vivo release of MPO from leukocytes.
Scheffer
Peter G
PG
Metabolic Laboratory, Department of Clinical Chemistry, VU University Medical Center, P.O. Box 7057, 1007 MB Amsterdam, The Netherlands. p.scheffer@vumc.nl
van der Zwan
Leonard P
LP
Schindhelm
Roger K
RK
Vermue
Hendrikus P A
HP
Teerlink
Tom
T
D000328:Adult; D000925:Anticoagulants / Q000494:pharmacology*; D001793:Blood Preservation / Q000379:methods; D004492:Edetic Acid / Q000494:pharmacology; D005260:Female; D006493:Heparin / Q000494:pharmacology; D006801:Humans; D008297:Male; D009195:Peroxidase / Q000097:blood*; D010949:Plasma / Q000187:drug effects* / Q000201:enzymology; D044967:Serum / Q000187:drug effects* / Q000201:enzymology; D013696:Temperature
D000925:Anticoagulants; D006493:Heparin; D004492:Edetic Acid; D009195:Peroxidase