DNA ligases from thermophilic bacteria enhance PCR amplification of long DNA sequences.
Bacterial NAD-dependent Taq and Tth DNA ligases are capable of significantly increasing the yield of long PCR products when the amplification is carried out using bacterial family A DNA polymerases, e.g. Taq or Tth DNA polymerases, or with enzymatic blends containing these polymerases. We also show that Taq and Tth DNA ligases improve the results of PCR in the absence of NAD and therefore in the absence of DNA ligase activity. These observations suggest that bacterial DNA ligases can interact with these DNA polymerases, presumably as accessory proteins, thereby enhancing the efficiency of DNA polymerization.
Ignatov
K B
KB
Vavilov Institute of General Genetics, Russian Academy of Sciences, Moscow, 119991, Russia. ignatovkb@bk.ru
Kramarov
V M
VM
D001426:Bacterial Proteins / Q000378:metabolism*; D004247:DNA / Q000235:genetics*; D011088:DNA Ligases / Q000378:metabolism*; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism*; D009243:NAD / Q000378:metabolism; D016133:Polymerase Chain Reaction; D019914:Taq Polymerase / Q000378:metabolism*; D013824:Thermus / Q000201:enzymology
D001426:Bacterial Proteins; D009243:NAD; D004247:DNA; D019914:Taq Polymerase; C068508:Tth polymerase; D004259:DNA-Directed DNA Polymerase; D011088:DNA Ligases