title: Genome-wide analysis of histone methylation reveals chromatin state-based regulation of gene transcription and function of memory CD8+ T cells.
abstract: Memory lymphocytes are characterized by their ability to exhibit a rapid response to the recall antigen, in which differential transcription is important, yet the underlying mechanism is not understood. We report here a genome-wide analysis of histone methylation on two histone H3 lysine residues (H3K4me3 and H3K27me3) and gene expression profiles in naive and memory CD8(+) T cells. We found that specific correlation exists between gene expression and the amounts of H3K4me3 (positive correlation) and H3K27me3 (negative correlation) across the gene body. These correlations displayed four distinct modes (repressive, active, poised, and bivalent), reflecting different functions of these genes in CD8(+) T cells. Furthermore, a permissive chromatin state of each gene was established by a combination of different histone modifications. Our findings reveal a complex regulation by histone methylation in differential gene expression and suggest that histone methylation may be responsible for memory CD8(+) T cell function.
authors.affiliation: Laboratory of Immunology, National Institute on Aging, National Institutes of Health, Baltimore, MD 21224, USA.
authors.forename: Yasuto
authors.initials: Y
authors.lastname: Araki
authors.forename: Zhibin
authors.initials: Z
authors.lastname: Wang
authors.forename: Chongzhi
authors.initials: C
authors.lastname: Zang
authors.forename: William H
authors.initials: WH
authors.lastname: Wood
authors.forename: Dustin
authors.initials: D
authors.lastname: Schones
authors.forename: Kairong
authors.initials: K
authors.lastname: Cui
authors.forename: Tae-Young
authors.initials: TY
authors.lastname: Roh
authors.forename: Brad
authors.initials: B
authors.lastname: Lhotsky
authors.forename: Robert P
authors.initials: RP
authors.lastname: Wersto
authors.forename: Weiqun
authors.initials: W
authors.lastname: Peng
authors.forename: Kevin G
authors.initials: KG
authors.lastname: Becker
authors.forename: Keji
authors.initials: K
authors.lastname: Zhao
authors.forename: Nan-ping
authors.initials: NP
authors.lastname: Weng
mesh_terms: D018414:CD8-Positive T-Lymphocytes / Q000276:immunology* / Q000378:metabolism; D002454:Cell Differentiation / Q000235:genetics* / Q000276:immunology; D002843:Chromatin / Q000276:immunology / Q000378:metabolism*; D020869:Gene Expression Profiling; D055106:Genome-Wide Association Study; D006657:Histones / Q000276:immunology / Q000378:metabolism*; D006801:Humans; D007156:Immunologic Memory / Q000235:genetics*; D008745:Methylation
chemical_list: D002843:Chromatin; D006657:Histones