title: Identification of inhibitors of chromatin modifying enzymes using the yeast phenotypic screens.
abstract: A multitude of enzymes that modify histones and remodel nucleosomes are required for the formation, maintenance, and propagation of the transcriptionally repressed chromatin state in eukaryotes. Robust phenotypic screens in yeast S. cerevisiae have proved instrumental in identifying these activities and for providing mechanistic insights into epigenetic regulation. These phenotypic assays, amenable for high throughput small molecule screening, enable identification and characterization of inhibitors of chromatin modifying enzymes largely bypassing traditional biochemical approaches.
authors.affiliation: Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA.
authors.forename: Benjamin
authors.initials: B
authors.lastname: Newcomb
authors.forename: Antonio
authors.initials: A
authors.lastname: Bedalov
mesh_terms: D002843:Chromatin / Q000187:drug effects / Q000201:enzymology / Q000235:genetics; D042002:Chromatin Assembly and Disassembly / Q000187:drug effects* / Q000235:genetics / Q000502:physiology; D003470:Culture Media / Q000737:chemistry; D004271:DNA, Fungal / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D004353:Drug Evaluation, Preclinical / Q000379:methods; D044127:Epigenesis, Genetic; D020868:Gene Silencing; D049770:Genes, Mating Type, Fungal; D006657:Histones / Q000378:metabolism; D010641:Phenotype; D012441:Saccharomyces cerevisiae / Q000187:drug effects* / Q000201:enzymology* / Q000235:genetics; D016615:Telomere / Q000235:genetics
chemical_list: D002843:Chromatin; D003470:Culture Media; D004271:DNA, Fungal; D004275:DNA, Ribosomal; D006657:Histones