title: Dexamethasone regulates expression of BRUCE/Apollon and the proliferation of neural progenitor cells.
abstract: Glucocorticoid hormones (GHs) regulate cell proliferation of neural progenitor cells (NPCs) contributing to reduction of neurogenesis after stress. We show here that dexamethasone (Dex) decreases BRUCE/Apollon (BRUCE) in cultured NPCs in a GH-receptor-dependent manner. Downregulation of BRUCE by Dex or using silencing RNA reduced the number of proliferating NPCs, whilst overexpression of BRUCE counteracted the effect of Dex. Dex also elevated the deubiquitinating enzyme, Usp8/Ubpy, which via Nrdp1 decreases BRUCE. The results show that BRUCE is a target for GHs in the NPCs, and that BRUCE controls cell division of NPCs and possibly of other stem cells.
authors.affiliation: Minerva Medical Research Institute, Biomedicum, Helsinki, Finland.
authors.forename: Maria
authors.initials: M
authors.lastname: Sippel
authors.forename: Raili
authors.initials: R
authors.lastname: Rajala
authors.forename: Laura
authors.initials: L
authors.lastname: Korhonen
authors.forename: Beat
authors.initials: B
authors.lastname: Bornhauser
authors.forename: Anna-Leena
authors.initials: AL
authors.lastname: Sokka
authors.forename: Mikihiko
authors.initials: M
authors.lastname: Naito
authors.forename: Dan
authors.initials: D
authors.lastname: Lindholm
mesh_terms: D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects*; D003907:Dexamethasone / Q000494:pharmacology*; D015536:Down-Regulation; D004622:Embryo, Mammalian / Q000378:metabolism; D005786:Gene Expression Regulation; D055495:Neurogenesis; D009474:Neurons / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D051381:Rats; D013234:Stem Cells / Q000166:cytology / Q000187:drug effects*; D044763:Ubiquitin-Conjugating Enzymes / Q000235:genetics* / Q000378:metabolism; D064570:Ubiquitin-Specific Proteases / Q000235:genetics / Q000378:metabolism
chemical_list: D003907:Dexamethasone; D044763:Ubiquitin-Conjugating Enzymes; C000598578:USP8 protein, rat; D064570:Ubiquitin-Specific Proteases; C506881:Birc6 protein, rat