Pyrrolizidine alkaloid isoline-induced oxidative injury in various mouse tissues.
Isoline is a retronecine-type pyrrolizidine alkaloid (PA) isolated from the traditional Chinese medicinal herb Ligularia duciformis. The present investigation was carried out to evaluate isoline-induced oxidative injury in various important mouse organs. Various tissue samples were collected after mice were administrated with 100mg/kg isoline for 36h, and then lipid peroxidation (LPO) level, total antioxidant capacity, glutathione-S-transferase (GST), glutathione peroxidase (GPx) and catalase (CAT) activities were determined to evaluate the oxidative injury. Our results showed that the total antioxidant capacity of liver, brain and lung were all decreased after given isoline, and the LPO level was increased in liver and heart of isoline-treated mice. Further antioxidant-related enzyme activity assays showed that isoline (100mg/kg) decreased GPx activity in liver and heart, increased CAT activity in liver, brain and heart, and decreased the GST activity in lung. Taken together, our results demonstrate that isoline can induce different oxidative injury in various important mouse organs, and of which liver is the most sensitive organ.
Liu
Tian-Yu
TY
Key Laboratory of Standardization of Chinese Medicines of Ministry of Education, Shanghai Key Laboratory of Complex Prescription, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, 1200 Cailun Road, Shanghai 201203, People's Republic of China.
Chen
Ying
Y
Wang
Zai-Yong
ZY
Ji
Li-Li
LL
Wang
Zheng-Tao
ZT
D000818:Animals; D001921:Brain / Q000187:drug effects*; D002374:Catalase / Q000187:drug effects; D005979:Glutathione Peroxidase / Q000187:drug effects; D005982:Glutathione Transferase / Q000187:drug effects; D006321:Heart / Q000187:drug effects*; D015227:Lipid Peroxidation / Q000187:drug effects; D008099:Liver / Q000187:drug effects*; D008168:Lung / Q000187:drug effects*; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D018384:Oxidative Stress / Q000187:drug effects*; D011763:Pyrrolizidine Alkaloids / Q000633:toxicity*
D011763:Pyrrolizidine Alkaloids; C489556:isoline; D002374:Catalase; D005979:Glutathione Peroxidase; D005982:Glutathione Transferase