Shift of oxidants and antioxidants levels in rats as a reaction to exposure to sulfur mustard.
Antixodants as well as oxidants levels were investigated in plasma of rats exposed to sulfur mustard in doses of 0 (control), 5, 20 and 80 mg kg(-1) body weight. Cyclic voltammetry performed on screen-printed strips with platinum working electrode used as a tool for assaying oxidant and antioxidant levels. We found significant shifts in both examined analytes. A dose of sulfur mustard of 5 mg kg(-1) body weight caused only a small change in oxidant and antioxidant levels when compared with the control group. A dose of 20 mg kg(-1) body weight provided a significant increase in antioxidants as well oxidants; however, the ratio of both was similar to that in the control group. The most surprising facts were found when the highest dose of 80 mg kg(-1) body weight of sulfur mustard was applied. While antioxidants were significantly increased, oxidants were decreased on an extensive scale.
Pohanka
Miroslav
M
Center of Advanced Studies, Faculty of Military Health Sciences, University of Defence, Hradec Kralove, Czech Republic. rau@atlas.cz
Stetina
Rudolf
R
D000279:Administration, Cutaneous; D000818:Animals; D000975:Antioxidants / Q000032:analysis*; D002619:Chemical Warfare Agents / Q000633:toxicity*; D004305:Dose-Response Relationship, Drug; D055664:Electrochemical Techniques; D005260:Female; D008297:Male; D009151:Mustard Gas / Q000008:administration & dosage / Q000633:toxicity*; D016877:Oxidants / Q000097:blood*; D051381:Rats; D017208:Rats, Wistar
D000975:Antioxidants; D002619:Chemical Warfare Agents; D016877:Oxidants; D009151:Mustard Gas