title: Evaluation of new preanalysis sample treatment tools and DNA isolation protocols to improve bacterial pathogen detection in whole blood.
abstract: Two novel preanalysis sample treatment tools were evaluated in combination with four DNA extraction kits for the selective isolation of bacterial DNA from whole blood. The combination of performing a preanalysis sample treatment and using a larger sample volume increased the detection limit to 50 CFU per ml.
authors.affiliation: Department of Medical Microbiology, Maastricht University Medical Center, The Netherlands.
authors.forename: Wendy L J
authors.initials: WL
authors.lastname: Hansen
authors.forename: Cathrien A
authors.initials: CA
authors.lastname: Bruggeman
authors.forename: Petra F G
authors.initials: PF
authors.lastname: Wolffs
mesh_terms: D001419:Bacteria / Q000235:genetics / Q000302:isolation & purification*; D001769:Blood / Q000382:microbiology*; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D011933:Reagent Kits, Diagnostic; D012680:Sensitivity and Specificity; D013048:Specimen Handling / Q000379:methods*
chemical_list: D004269:DNA, Bacterial; D011933:Reagent Kits, Diagnostic