Pacific ciguatoxin 1B-induced modulation of inflammatory mediators in a murine macrophage cell line.
Ciguatoxins, potent marine neurotoxins responsible for ciguatera, exert their numerous damaging effects through primary binding to the voltage-sensitive sodium channels of excitable cells. Using RAW 264.7 murine macrophages, we report the first experimental study presenting evidence that P-CTX-1B (the most potent congener from the Pacific) could modulate mRNA expression of pro- and anti-inflammatory cytokines as well as of inducible nitric oxide synthase (iNOS). P-CTX-1B, unlike other less potent marine polyether toxins, P-CTX-3C and PbTx-3, induced the overexpression of interleukin (IL)-1beta, IL-6, IL-10, tumor necrosis factor-alpha and iNOS with different magnitude and kinetic profiles, as compared to bacterial lipopolysaccharide (LPS). Unlike LPS, P-CTX-1B did not modulate IL-11 expression. In this report, we provide new evidence of the P-CTX-1B iNOS- and cytokines-inducing ability and shed new light on host response to potent neurotoxins.
Matsui
Mariko
M
Laboratoire des Biotoxines, Institut Pasteur de Nouvelle-Calédonie, Noumea, New Caledonia.
Kumar-Roine
Shilpa
S
Darius
H Taiana
HT
Chinain
Mireille
M
Laurent
Dominique
D
Pauillac
Serge
S
D000818:Animals; D002460:Cell Line; D002922:Ciguatoxins / Q000633:toxicity*; D004790:Enzyme Induction; D018836:Inflammation Mediators / Q000378:metabolism*; D008070:Lipopolysaccharides / Q000494:pharmacology; D008264:Macrophages / Q000187:drug effects* / Q000378:metabolism; D051379:Mice; D052247:Nitric Oxide Synthase Type II / Q000096:biosynthesis; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000235:genetics
D018836:Inflammation Mediators; D008070:Lipopolysaccharides; D012333:RNA, Messenger; C454647:ciguatoxin 1B (CTX 1B); D002922:Ciguatoxins; D052247:Nitric Oxide Synthase Type II