A genome map of Salmonella enterica serovar Agona: numerous insertions and deletions reflecting the evolutionary history of a human pathogen.
Salmonella enterica serovar Agona is an important zoonotic pathogen, causing serious human illness worldwide, but knowledge about its genetics and evolution, especially regarding the genomic events that might have contributed to the formation of S. Agona as an important pathogen, is lacking. As a first step toward understanding this pathogen and characterizing its genomic differences with other salmonellae, we constructed a physical map of S. Agona in strain SARB1 using I-CeuI, XbaI, AvrII and Tn10 insertions with pulsed-field gel electrophoresis techniques. On the 4815-kb genomic map, we located 82 genes, revealed one inversion of about 1000 kb and resolved seven deletions and seven insertions ranging from 10 to 67 kb relative to the genome of Salmonella typhimurium LT2. These genomic features clearly distinguish S. Agona from other previously analyzed salmonellae and provide clues to the molecular basis for its genomic divergence. Additionally, these kinds of physical maps, combined with emerging high-speed sequencing technologies, such as the Solexa or SOLiD techniques, which require a pre-existing high-resolution physical map such as the S. Agona map reported here, will play important roles in genomic comparative studies of bacteria involving large numbers of strains.
Chen
Fang
F
Department of Microbiology, Peking University Health Science Center, Beijing, China.
Poppe
Cornelis
C
Liu
Gui-Rong
GR
Li
Yong-Guo
YG
Peng
Yi-Hong
YH
Sanderson
Kenneth E
KE
Johnston
Randal N
RN
Liu
Shu-Lin
SL
D007446:Chromosome Inversion; D004251:DNA Transposable Elements; D004269:DNA, Bacterial / Q000235:genetics*; D016521:Electrophoresis, Gel, Pulsed-Field; D019143:Evolution, Molecular; D016680:Genome, Bacterial; D006801:Humans; D016254:Mutagenesis, Insertional; D011995:Recombination, Genetic; D015183:Restriction Mapping; D019779:Salmonella enterica / Q000235:genetics*; D012486:Salmonella typhimurium / Q000235:genetics; D017384:Sequence Deletion
D004251:DNA Transposable Elements; D004269:DNA, Bacterial