title: Investigation of biological activity of polar extracts isolated from Phlomis crinita Cav ssp. mauritanica Munby.
abstract: The lyophilized infusion, the methanol, the ethyl acetate, and the total oligomer flavonoid (TOF)-enriched extracts prepared from the dried leaves of Phlomis crinita Cav. ssp. mauritanica Munby were investigated for the contents of flavonoids, tannins, coumarines and steroids. Antibacterial activity was investigated toward five bacterial strains. An inhibitory effect was observed against Staphyllococcus aureus and Enterococcus feacalis, and the minimal inhibitory concentrations ranged from 2.5 to 5 mg/mL of extract. The tested extracts exhibit an important free radical scavenging activity toward the 1,1-diphenyl 2-picrylhydrazyl (DPPH) free radical; with IC(50) values of 30.5, 6, 32, and 31.5 microg/mL, respectively, in the presence of lyophilized infusion, the TOF, the methanol, and the ethyl acetate extracts. Genotoxic and antigenotoxic properties of the different extracts were studied by using the SOS chromotest with Escherichia coli PQ37. The lyophilized infusion and TOF extracts obtained from P. crinita ssp. mauritanica showed no genotoxicity, whereas methanol and ethyl acetate extracts are considered as marginally genotoxic. On the other hand, we showed that each extract inhibited the mutagenicity induced by aflatoxin B1 (AFB1) (10 microg/assay) and nifuroxazide (NF) (10 microg/assay). The ethyl acetate extract showed the strongest level of protection toward the genotoxicity induced by both directly and indirectly genotoxic NF and AFB1. These tests proved that the lyophilized infusion possesses an antiradical activity likewise, it showed no genotoxic effect; that is why we choose this extract to assess its antiulcerogenic activity by using an ethanol-induced ulcerogenesis model in the rat. This test demonstrates that 300 mg/kg of a P. crinita ssp. mauritanica lyophilized infusion was more effective than the reference compound, cimetidine.
authors.affiliation: Laboratory of Cellular and Molecular Biology, Faculty of Dental Medicine, Monastir, Tunisia.
authors.forename: Ilef
authors.initials: I
authors.lastname: Limem-Ben Amor
authors.forename: Ines
authors.initials: I
authors.lastname: Skandrani
authors.forename: Jihed
authors.initials: J
authors.lastname: Boubaker
authors.forename: Mohamed
authors.initials: M
authors.lastname: Ben Sghaïer
authors.forename: Aicha
authors.initials: A
authors.lastname: Neffati
authors.forename: Wissem
authors.initials: W
authors.lastname: Bhouri
authors.forename: Ines
authors.initials: I
authors.lastname: Bouhlel
authors.forename: Nabil
authors.initials: N
authors.lastname: Chouchane
authors.forename: Soumaya
authors.initials: S
authors.lastname: Kilani
authors.forename: Emmanuel
authors.initials: E
authors.lastname: Guedon
authors.forename: Mohamed
authors.initials: M
authors.lastname: Ghoul
authors.forename: Kamel
authors.initials: K
authors.lastname: Ghedira
authors.forename: Leila
authors.initials: L
authors.lastname: Chekir-Ghedira
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology*; D000897:Anti-Ulcer Agents / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology*; D016587:Antimutagenic Agents / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology; D002927:Cimetidine / Q000494:pharmacology; D005260:Female; D016166:Free Radical Scavengers / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology; D005612:Freeze Drying; D020128:Inhibitory Concentration 50; D008297:Male; D008826:Microbial Sensitivity Tests; D009152:Mutagenicity Tests; D031345:Phlomis / Q000737:chemistry*; D010936:Plant Extracts / Q000008:administration & dosage / Q000494:pharmacology*; D018515:Plant Leaves; D051381:Rats
chemical_list: D000900:Anti-Bacterial Agents; D000897:Anti-Ulcer Agents; D016587:Antimutagenic Agents; D016166:Free Radical Scavengers; D010936:Plant Extracts; D002927:Cimetidine