title: Circulation of Streptococcus pneumoniae clone Colombia 5 ST289 in nine Latin American countries.
abstract: OBJECTIVE
  To determine genetic relatedness of clone Colombia(5) ST289 with invasive Streptococcus pneumoniae serotype 5 isolates recovered in nine Latin American countries.
  
  
  METHODS
  Forty-four invasive S. pneumoniae serotype 5 isolates recovered from children under 5 years of age in Bolivia, Chile, Dominican Republic, Ecuador, Nicaragua, Panama, Paraguay, Peru, and Venezuela were studied. Pulsed-field gel electrophoresis patterns of DNA treated with SmaI restriction enzyme were classified using Tenover's criteria and analyzed with the Fingerprinting II program to determine their genetic relatedness with the Colombian clone.
  
  
  RESULTS
  All isolates had a genetic similarity of 78.5% or more with the Colombian clone. Thirteen electrophoretic subtypes derived of pattern A were identified, and five of them (A5, A6, A8, A13, A27) comprised 61.4% of the isolates.
  
  
  CONCLUSIONS
  Clone Colombia(5) ST289 is disseminated in Latin America. This is important because S. pneumoniae serotype 5 frequently causes invasive disease in the region and is associated with trimethoprim-sulfamethoxazole resistance.
authors.affiliation: Grupo de Microbiología, Instituto Nacional de Salud, Bogotá, Colombia.
authors.forename: Carolina
authors.initials: C
authors.lastname: Firacative
authors.forename: Jaime
authors.initials: J
authors.lastname: Moreno
authors.forename: Patricia
authors.initials: P
authors.lastname: Rosales
authors.forename: Aurora
authors.initials: A
authors.lastname: Maldonado
authors.forename: Jacqueline
authors.initials: J
authors.lastname: Sánchez
authors.forename: Carmen
authors.initials: C
authors.lastname: Pesantes
authors.forename: Sergio
authors.initials: S
authors.lastname: López
authors.forename: Markela
authors.initials: M
authors.lastname: de Quinzada
authors.forename: Gustavo
authors.initials: G
authors.lastname: Chamorro
authors.forename: Sara
authors.initials: S
authors.lastname: Morales
authors.forename: Enza
authors.initials: E
authors.lastname: Spadola
authors.forename: Jean-Marc
authors.initials: JM
authors.lastname: Gabastou
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Castañeda
mesh_terms: D003105:Colombia; D006801:Humans; D007843:Latin America; D013296:Streptococcus pneumoniae / Q000235:genetics* / Q000302:isolation & purification*