title: Fluorescence logic-signal-based multiplex detection of nucleases with the assembly of a cationic conjugated polymer and branched DNA.
abstract: An energy-transfer cascade is generated from a cationic conjugated polymer (PFP) and negatively charged, Y-shaped DNA labeled with three dyes at its termini (fluorescein (Fl), Tex Red, and Cy5). Multistep fluorescence resonance energy transfer regulates the fluorescence intensities of PFP and the dyes. Different types of logic gates can be operated by observing the emission wavelengths of different dyes with multiplex nucleases as inputs.
authors.affiliation: Beijing National Laboratory for Molecular Sciences, Key Laboratory of Organic Solids, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190 PR China.
authors.forename: Xuli
authors.initials: X
authors.lastname: Feng
authors.forename: Xinrui
authors.initials: X
authors.lastname: Duan
authors.forename: Libing
authors.initials: L
authors.lastname: Liu
authors.forename: Fude
authors.initials: F
authors.lastname: Feng
authors.forename: Shu
authors.initials: S
authors.lastname: Wang
authors.forename: Yuliang
authors.initials: Y
authors.lastname: Li
authors.forename: Daoben
authors.initials: D
authors.lastname: Zhu
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D002412:Cations / Q000737:chemistry*; D004247:DNA / Q000737:chemistry*; D004262:DNA Restriction Enzymes / Q000032:analysis*; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods*; D005456:Fluorescent Dyes; D009690:Nucleic Acid Conformation; D011108:Polymers / Q000737:chemistry*
chemical_list: D002412:Cations; D005456:Fluorescent Dyes; D011108:Polymers; D004247:DNA; D004262:DNA Restriction Enzymes