Quantum dots-gold(III)-based indirect fluorescence immunoassay for high-throughput screening of APP.
Here we report a indirect fluorescence immunoassay for high-throughput screening of APP based on the fluorescence quenching of quantum dots by gold(III), which were dissolved from gold nanoparticles-Rabbit anti-Pig IgG conjugate, and further utilize this system to detect APP in pig serum with high sensitivity and specificity.
Sheng
Zonghai
Z
Huazhong Agricultural University, Wuhan, PR China.
Han
Heyou
H
Hu
Dehong
D
Liang
Jiangong
J
He
Qigai
Q
Jin
Meilin
M
Zhou
Rui
R
Chen
Huanchun
H
D016977:Actinobacillus pleuropneumoniae / Q000302:isolation & purification*; D000818:Animals; D019084:Fluorescent Antibody Technique, Indirect / Q000379:methods*; D005978:Glutathione / Q000737:chemistry; D006046:Gold / Q000737:chemistry*; D045663:Quantum Dots; D012680:Sensitivity and Specificity; D012995:Solubility; D013552:Swine / Q000097:blood / Q000382:microbiology; D014867:Water / Q000737:chemistry
D014867:Water; D006046:Gold; D005978:Glutathione