Microfabricated tissue gauges to measure and manipulate forces from 3D microtissues.
Physical forces generated by cells drive morphologic changes during development and can feedback to regulate cellular phenotypes. Because these phenomena typically occur within a 3-dimensional (3D) matrix in vivo, we used microelectromechanical systems (MEMS) technology to generate arrays of microtissues consisting of cells encapsulated within 3D micropatterned matrices. Microcantilevers were used to simultaneously constrain the remodeling of a collagen gel and to report forces generated during this process. By concurrently measuring forces and observing matrix remodeling at cellular length scales, we report an initial correlation and later decoupling between cellular contractile forces and changes in tissue morphology. Independently varying the mechanical stiffness of the cantilevers and collagen matrix revealed that cellular forces increased with boundary or matrix rigidity whereas levels of cytoskeletal and extracellular matrix (ECM) proteins correlated with levels of mechanical stress. By mapping these relationships between cellular and matrix mechanics, cellular forces, and protein expression onto a bio-chemo-mechanical model of microtissue contractility, we demonstrate how intratissue gradients of mechanical stress can emerge from collective cellular contractility and finally, how such gradients can be used to engineer protein composition and organization within a 3D tissue. Together, these findings highlight a complex and dynamic relationship between cellular forces, ECM remodeling, and cellular phenotype and describe a system to study and apply this relationship within engineered 3D microtissues.
Legant
Wesley R
WR
Department of Bioengineering, University of Pennsylvania, Philadelphia, PA 19104, USA.
Pathak
Amit
A
Yang
Michael T
MT
Deshpande
Vikram S
VS
McMeeking
Robert M
RM
Chen
Christopher S
CS
D000818:Animals; D003599:Cytoskeleton; D016326:Extracellular Matrix Proteins / Q000737:chemistry; D051379:Mice; D046228:Microarray Analysis; D008904:Miniaturization; D041681:NIH 3T3 Cells; D046509:Tissue Culture Techniques; D016610:Tissue Embedding / Q000379:methods*; D023822:Tissue Engineering
D016326:Extracellular Matrix Proteins