title: Multiple dual-mode centrifugal partition chromatography as an efficient method for the purification of a mycosporine from a crude methanolic extract of Lichina pygmaea.
abstract: Centrifugal partition chromatography method was applied to the separation and purification of a crude methanolic extract of a cyanobacterial lichen, Lichina pygmaea. A multiple dual-mode was used to separate two compounds of interest, namely mycosporine-serinol and a glutamic acid derivative. These compounds are described here for the first time in a lichen. Their structures were identified by UV, IR, ESI-MS, (1)H NMR, (13)C NMR, and 2D NMR.
authors.affiliation: EA 4090 Substances lichéniques et photoprotection, UFR Sciences pharmaceutiques et Biologiques, Université Européenne de Bretagne, Université de Rennes 1, 2 Av. du Pr. Léon Bernard, 35043 Rennes Cedex, France.
authors.forename: Catherine
authors.initials: C
authors.lastname: Roullier
authors.forename: Marylène
authors.initials: M
authors.lastname: Chollet-Krugler
authors.forename: Aurélie
authors.initials: A
authors.lastname: Bernard
authors.forename: Joël
authors.initials: J
authors.lastname: Boustie
mesh_terms: D001203:Ascomycota / Q000737:chemistry*; D002853:Chromatography, Liquid / Q000379:methods*; D000458:Cyanobacteria / Q000737:chemistry*; D003512:Cyclohexanones / Q000737:chemistry / Q000302:isolation & purification*; D008011:Lichens / Q000737:chemistry*; D000432:Methanol / Q000737:chemistry; D011409:Propylene Glycols / Q000737:chemistry / Q000302:isolation & purification*
chemical_list: D003512:Cyclohexanones; D011409:Propylene Glycols; C544070:mycosporine-serinol; D000432:Methanol