[Morphological features of regenerative-reparative processes in the cornea exposed to synthetic analogues of endogenous peptide fragments of protein S100b in an experiment].
The effects of two 5-membered (SP5, SP6) endogenous synthetic peptide fragments of protein S100b on the course of regenerative-reparative processes in the corneas of Wistar rats were morphologically studied after making a graduated injury. Conjunctival or endonasal injection of the peptides was made at two concentrations of 10(-6) and 10(-8) M. The study peptides were found to have a modulating effect on regenerative-reparative processes regardless of the route of administration and to induce accelerated and qualitative corneal wound healing as compared with the controls. The specific feature of SP5 was the formation of a fibroblastic membrane. When SP5 was injected at a concentration of 10(-6) M, the membrane was more pronounced and occupied the whole area of keratectomy whereas when this peptide was administered at a concentration of 10(-8) M, its formation was observed in the deep and middle corneal layers. The most marked effect of SP6 revealed during the study was the formation of newly-formed vessels over the entire wound surface independently of the concentration of the injected substance. Of the two study peptide fragments, SP5 is, in the authors' opinion, promising for clinical application. Significant keratocytic proliferation noted in the use of SP5 at a concentration of 10(-6) M may be used to accelerate scarring when the sclera and cutaneous covering are damaged. The effects detected with administration of SP6 at two concentrations were regarded as negative as they did not answer the purpose set during the study performed.
Olinevich
V B
VB
Ziangirova
G G
GG
Mamikonian
V R
VR
Gruden'
M A
MA
Sherstnev
V V
VV
D000818:Animals; D001324:Autoantigens; D003315:Cornea / Q000502:physiology*; D003316:Corneal Diseases / Q000473:pathology / Q000503:physiopathology*; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D008297:Male; D009414:Nerve Growth Factors / Q000008:administration & dosage*; D009883:Ophthalmic Solutions; D010446:Peptide Fragments / Q000008:administration & dosage*; D051381:Rats; D017208:Rats, Wistar; D012038:Regeneration / Q000502:physiology*; D064568:S100 Calcium Binding Protein beta Subunit; D009418:S100 Proteins / Q000008:administration & dosage*; D016896:Treatment Outcome
D001324:Autoantigens; D009414:Nerve Growth Factors; D009883:Ophthalmic Solutions; D010446:Peptide Fragments; D064568:S100 Calcium Binding Protein beta Subunit; D009418:S100 Proteins; C579158:S100b protein, rat