title: Iron transporters are differentially regulated by dietary iron, and modifications are associated with changes in manganese metabolism in young pigs.
abstract: To investigate the effects of dietary iron (Fe) on manganese (Mn) metabolism, 24 weaned pigs (21 d old) were blocked by litter and weight and randomly assigned to the following treatments: 1) no supplemental Fe [low Fe (L-Fe)]; 2) 100 mg supplemental Fe/kg [adequate Fe (A-Fe)]; and 3) 500 mg supplemental Fe/kg [high Fe (H-Fe)]. The basal diet was analyzed to contain 20 mg Fe/kg. Tissues were harvested after 32 d of feeding. Daily gain (least square means +/- SEM) was greater in A-Fe pigs (328.3 +/- 29.9 g/d) than in L-Fe pigs (224.0 +/- 11.2 g/d). Hemoglobin concentrations on d 32 were lower in L-Fe pigs (62 +/- 3.5 g/L) than in A-Fe pigs (128 +/- 5.6 g/L) and did not differ between pigs fed A-Fe and H-Fe (133 +/- 12.0 g/L). Liver Fe increased with increasing dietary Fe. Relative hepatic hepcidin expression was greater in pigs fed A-Fe and H-Fe than in those fed L-Fe. Relative expressions of duodenal divalent metal transporter 1 (DMT1) and solute carrier family 39 member 14 (ZIP14) were increased in L-Fe pigs compared with H-Fe pigs. Liver copper (Cu) was higher in L-Fe (0.56 +/- 0.04 mmol/kg) and H-Fe (0.58 +/- 0.04 mmol/kg) pigs than in A-Fe pigs (0.40 +/- 0.04 mmol/kg). Liver Mn was lower in H-Fe pigs (0.15 +/- 0.01 mmol/kg) than in A-Fe (0.23 +/- 0.02 mmol/kg) or L-Fe pigs (0.20 +/- 0.02 mmol/kg). Duodenal Mn concentrations were greater in L-Fe pigs than in A-Fe or H-Fe pigs. Fe deficiency in pigs increased gene expression of duodenal metal transporters (DMT1 and ZIP14) and supplementation with H-Fe reduced expression of DMT1 and ZIP14, which may have decreased absorption of Mn.
authors.affiliation: Department of Animal Science, North Carolina State University, Raleigh, NC 27695, USA.
authors.forename: Stephanie L
authors.initials: SL
authors.lastname: Hansen
authors.forename: Nares
authors.initials: N
authors.lastname: Trakooljul
authors.forename: Hsiao-Ching
authors.initials: HC
authors.lastname: Liu
authors.forename: Adam J
authors.initials: AJ
authors.lastname: Moeser
authors.forename: Jerry W
authors.initials: JW
authors.lastname: Spears
mesh_terms: D000818:Animals; D023181:Antimicrobial Cationic Peptides / Q000378:metabolism; D027682:Cation Transport Proteins / Q000235:genetics / Q000378:metabolism*; D003300:Copper / Q000378:metabolism; D019587:Dietary Supplements; D004386:Duodenum / Q000378:metabolism; D015870:Gene Expression; D006454:Hemoglobins / Q000378:metabolism; D064451:Hepcidins; D017136:Ion Transport / Q000187:drug effects*; D007501:Iron / Q000378:metabolism*; D019266:Iron, Dietary / Q000494:pharmacology*; D008099:Liver / Q000378:metabolism; D008297:Male; D008345:Manganese / Q000378:metabolism*; D013552:Swine; D014131:Trace Elements / Q000494:pharmacology; D015430:Weight Gain / Q000187:drug effects; D000099236:Solute Carrier Family 11, Member 2
chemical_list: D023181:Antimicrobial Cationic Peptides; D027682:Cation Transport Proteins; D003300:Copper; D006454:Hemoglobins; D064451:Hepcidins; D007501:Iron; D019266:Iron, Dietary; D008345:Manganese; D014131:Trace Elements; D000099236:Solute Carrier Family 11, Member 2