Structured illumination microscopy of a living cell.
Due to diffraction, the resolution of imaging emitted light in a fluorescence microscope is limited to about 200 nm in the lateral direction. Resolution improvement by a factor of two can be achieved using structured illumination, where a fine grating is projected onto the sample, and the final image is reconstructed from a set of images taken at different grating positions. Here we demonstrate that with the help of a spatial light modulator, this technique can be used for imaging slowly moving structures in living cells.
Hirvonen
Liisa M
LM
Randall Division of Cell and Molecular Biophysics, King's College London, New Hunt's House, London, UK.
Wicker
Kai
K
Mandula
Ondrej
O
Heintzmann
Rainer
R
D000465:Algorithms; D000818:Animals; D019556:COS Cells; D002522:Chlorocebus aethiops; D007091:Image Processing, Computer-Assisted / Q000379:methods; D008027:Light; D008856:Microscopy, Fluorescence / Q000295:instrumentation / Q000379:methods*; D008863:Microspheres; D008928:Mitochondria / Q000502:physiology* / Q000648:ultrastructure*; D009038:Motion; D011137:Polystyrenes; D013997:Time Factors
D011137:Polystyrenes