Esterase D is essential for protection of Neisseria gonorrhoeae against nitrosative stress and for bacterial growth during interaction with cervical epithelial cells.
estD encodes a carboxylic ester hydrolase and is part of the NmlR regulon in Neisseria gonorrhoeae. An estD mutant was found to be susceptible to nitrite and to S-nitrosoglutathione. This mutant was also unable to infect and survive within human cervical epithelial cells, and it showed reduced ability to form a biofilm on these cells. We conclude that esterase D is an integral part of the nitrosative stress defense system of N. gonorrhoeae and that it has potential importance in pathogenesis.
Potter
Adam J
AJ
The University of Queensland, Brisbane, Queensland, Australia.
Kidd
Stephen P
SP
Edwards
Jennifer L
JL
Falsetta
Megan L
ML
Apicella
Michael A
MA
Jennings
Michael P
MP
McEwan
Alastair G
AG
D018441:Biofilms; D002584:Cervix Uteri / Q000166:cytology* / Q000382:microbiology; D004847:Epithelial Cells / Q000382:microbiology*; D005260:Female; D015964:Gene Expression Regulation, Bacterial / Q000502:physiology; D015971:Gene Expression Regulation, Enzymologic; D006801:Humans; D009344:Neisseria gonorrhoeae / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development*; D015538:Nitrosation; D012977:Sodium Nitrite / Q000494:pharmacology; D013869:Thiolester Hydrolases / Q000235:genetics / Q000378:metabolism*; D014774:Virulence
D013869:Thiolester Hydrolases; C020149:s-formylglutathione hydrolase; D012977:Sodium Nitrite