p21(Waf1/Cip1) as a molecular sensor for BoHV-4 replication.
BoHV-4 replication cycle is dependent on the S-phase of the cell-cycle at the stage of viral DNA synthesis. Because p21 is a rate-limiting regulator of the G1/S-phase transition and up-regulated by DNA-damaging agents, in this study p21 expression in BoHV-4 infected cells was investigated. The p21 promoter was found to be highly activated in a dose- and time-dependent manner following BoHV-4 infection only in cells which are permissive for BoHV-4 replication. Thus p21 expression reports on BoHV-4 replication and could represent a host cell defensive response to infection-associated cellular damage.
Capocefalo
A
A
Facoltà di Medicina Veterinaria, Dipartimento di Salute Animale, Sezione di Malattie Infettive degli Animali, Parma, Italy.
Franceschi
V
V
Whitelaw
C B A
CB
Vasey
D B
DB
Lillico
S G
SG
Cavirani
S
S
Donofrio
G
G
D000818:Animals; D015415:Biomarkers; D002417:Cattle; D002418:Cattle Diseases / Q000378:metabolism / Q000821:virology*; D002460:Cell Line; D050759:Cyclin-Dependent Kinase Inhibitor p21 / Q000096:biosynthesis*; D017930:Genes, Reporter; D006566:Herpesviridae Infections / Q000378:metabolism / Q000662:veterinary* / Q000821:virology; D026021:Herpesvirus 4, Bovine / Q000502:physiology*; D054884:Host-Pathogen Interactions; D051379:Mice; D011401:Promoter Regions, Genetic; D013997:Time Factors; D014412:Tumor Virus Infections / Q000378:metabolism / Q000662:veterinary* / Q000821:virology; D014779:Virus Replication
D015415:Biomarkers; D050759:Cyclin-Dependent Kinase Inhibitor p21