title: Dynamin participates in the maintenance of anterior polarity in the Caenorhabditis elegans embryo.
abstract: Cell polarity is crucial for the generation of cell diversity. Recent evidence suggests that the actin cytoskeleton plays a key role in establishment of embryonic polarity, yet the mechanisms that maintain polarity cues in particular membrane domains during development remain unclear. Dynamin, a large GTPase, functions in both endocytosis and actin dynamics. Here, the Caenorhabditis elegans dynamin ortholog, DYN-1, maintains anterior polarity cues. DYN-1-GFP foci are enriched in the anterior cortex in a manner dependent on the anterior polarity proteins, PAR-6 and PKC-3. Membrane internalization and actin comet formation are enriched in the anterior, and are dependent on DYN-1. PAR-6-labeled puncta are also internalized from cortical accumulations of DYN-1-GFP. Our results demonstrate a mechanism for the spatial and temporal regulation of endocytosis in the anterior of the embryo, contributing to the precise localization and maintenance of polarity factors within a dynamic plasma membrane.
authors.affiliation: Department of Genetics, University of Wisconsin-Madison, Madison, WI 53706, USA.
authors.forename: Yuji
authors.initials: Y
authors.lastname: Nakayama
authors.forename: Jessica M
authors.initials: JM
authors.lastname: Shivas
authors.forename: Daniel S
authors.initials: DS
authors.lastname: Poole
authors.forename: Jayne M
authors.initials: JM
authors.lastname: Squirrell
authors.forename: Jennifer M
authors.initials: JM
authors.lastname: Kulkoski
authors.forename: Justin B
authors.initials: JB
authors.lastname: Schleede
authors.forename: Ahna R
authors.initials: AR
authors.lastname: Skop
mesh_terms: D000199:Actins / Q000378:metabolism; D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D017173:Caenorhabditis elegans / Q000166:cytology* / Q000196:embryology*; D029742:Caenorhabditis elegans Proteins / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism; D016764:Cell Polarity; D034281:Dynamins / Q000378:metabolism*; D004625:Embryo, Nonmammalian / Q000166:cytology* / Q000201:enzymology; D004705:Endocytosis; D017353:Gene Deletion; D021381:Protein Transport; D008941:Spindle Apparatus / Q000378:metabolism
chemical_list: D000199:Actins; D015415:Biomarkers; D029742:Caenorhabditis elegans Proteins; C511792:Dyn-1 protein, C elegans; D034281:Dynamins