Purification and enzymatic characterization of two beta-endoxylanases from Trichoderma sp. K9301 and their actions in xylooligosaccharide production.
Trichoderma sp. K9301 secreting endoxylanases with an activity of 2836 U/g (dry weight) was screened for XOs production. Two acidic beta-endoxylanases EX1 (30.1 kDa) and EX2 (20.1 kDa) were purified from crude extract of the strain K9301 in solid fermentation. Action modes of EX1 and EX2 towards XOs showed similar hydrolysis characters to endoxylanases belonging to glycosyl hydrolase family 10 and 11, respectively. EX1 exhibited better affinity but lower hydrolytic efficiency than EX2 to xylans from beechwood, birchwood, and oat-spelt. They had synergistic action on xylan hydrolysis. The optimum condition to prepare XOs from corncobs was obtained as 10 mg/ml corncob xylan incubated with 10 U/mg crude enzymes at 50 degrees C for 3 h. The yield of XOs reached 43.3%, and only a little amount of xylose (3.1%) was simultaneously produced, suggesting the good potential of strain K9301 in XOs production.
Chen
Lei-Lei
LL
The State Key Lab of Microbial Technology, Marine Biotechnology Research Center, Shandong University, Jinan 250100, PR China.
Zhang
Min
M
Zhang
Dong-Hua
DH
Chen
Xiu-Lan
XL
Sun
Cai-Yun
CY
Zhou
Bai-Cheng
BC
Zhang
Yu-Zhong
YZ
D018554:Avena / Q000378:metabolism; D045424:Complex Mixtures / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D043364:Endo-1,4-beta Xylanases / Q000302:isolation & purification*; D004795:Enzyme Stability; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D007700:Kinetics; D013696:Temperature; D013997:Time Factors; D014242:Trichoderma / Q000201:enzymology*; D014990:Xylans / Q000096:biosynthesis*; D003313:Zea mays / Q000378:metabolism
D045424:Complex Mixtures; D014990:Xylans; D043364:Endo-1,4-beta Xylanases