[Omega-3 polyunsaturated fatty acids stimulate the expression of PPAR target genes].
In experiments on isolated neonatal cardiomyocytes and hearts of adult rats we investigated the influence of polyunsaturated fatty acids (PUFA) on expression of two peroxisome proliferator-activated receptor (PPAR) target genes--fatty acid transport protein (FATP) and interleukin-1 receptor antagonist (IL-1ra). We determined that cell membranes of adult rats heart and cardiomyocytes of neonatal rats which were born by females fed with omega-3 PUFA for 4 weeks, contain more omega-3 PUFA (alpha-linolenic and docosahexaenoic acids) in comparison with the control. It was shown that omega-3 PUFA elevated FATP and IL-1ra mRNA expression in 2.25 (P = 0.07) and 8.4 (P < 0.00001) folds correspondingly in adult rats tissues. In isolated cardiomyocytes, the mRNA expression of FATP and IL-1ra were augmented 2.5 folds (P = 0.22) and 8.1 folds (P < 0.00001), accordingly. Our data show that omega-3 PUFA stimulate PPAR target genes expression and allow to permit genetic mechanisms in cardioprotective effects of omega-3 PUFA.
Kaplins'kyĭ
S P
SP
Shysh
A M
AM
Nahibin
V S
VS
Dosenko
V Ie
VIe
Klimashevs'kyĭ
V M
VM
Moĭbenko
O O
OO
D000818:Animals; D000831:Animals, Newborn; D002316:Cardiotonic Agents / Q000494:pharmacology*; D050612:Fatty Acid Transport Proteins / Q000235:genetics*; D015525:Fatty Acids, Omega-3 / Q000494:pharmacology*; D005260:Female; D015870:Gene Expression / Q000187:drug effects*; D006321:Heart / Q000187:drug effects / Q000196:embryology / Q000254:growth & development; D053590:Interleukin 1 Receptor Antagonist Protein / Q000235:genetics*; D008297:Male; D018811:Maternal Exposure; D032383:Myocytes, Cardiac / Q000187:drug effects* / Q000378:metabolism; D047492:Peroxisome Proliferator-Activated Receptors / Q000235:genetics*; D011247:Pregnancy; D051381:Rats; D017208:Rats, Wistar
D002316:Cardiotonic Agents; D050612:Fatty Acid Transport Proteins; D015525:Fatty Acids, Omega-3; D053590:Interleukin 1 Receptor Antagonist Protein; D047492:Peroxisome Proliferator-Activated Receptors