Enhanced saccharification of alkali-treated rice straw by cellulase from Trametes hirsuta and statistical optimization of hydrolysis conditions by RSM.
A white rot fungus, identified as Trametes hirsuta based on morphological and phylogenetic analysis, was found to contain efficient cellulose degrading enzymes. The strain showed maximum endoglucanase (EG), cellobiohydrolase (CBH) and beta-glucosidase (BGL) activities of 55, 0.28 and 5.0 U/mg-protein, respectively. Rice straw was found to be a potentially good substrate for growth of T. hirsuta for cellulase production. Statistical experimental design was used to optimize hydrolysis parameters such as pH, temperature, and concentrations of substrates and enzymes to achieve the highest saccharification yield. Enzyme concentration was identified as the limiting factor for saccharification of rice straw. A maximum saccharification rate of 88% was obtained at an enzyme concentration of 37.5 FPU/g-substrate after optimization of the hydrolysis parameters. The results of a confirmation experiment under the optimum conditions agreed well with model predictions. T. hirsuta may be a good choice for the production of reducing sugars from cellulosic biomass.
Jeya
Marimuthu
M
Department of Chemical Engineering, Konkuk University, Seoul 143-701, Republic of Korea.
Zhang
Ye-Wang
YW
Kim
In-Won
IW
Lee
Jung-Kul
JK
D001709:Biotechnology / Q000379:methods*; D050260:Carbohydrate Metabolism / Q000187:drug effects*; D002244:Carbon / Q000494:pharmacology; D002480:Cellulase / Q000378:metabolism*; D043366:Cellulose 1,4-beta-Cellobiosidase / Q000378:metabolism; D006868:Hydrolysis / Q000187:drug effects; D008954:Models, Biological; D009584:Nitrogen / Q000494:pharmacology; D012275:Oryza / Q000378:metabolism*; D010802:Phylogeny; D012044:Regression Analysis; D015203:Reproducibility of Results; D012972:Sodium Hydroxide / Q000494:pharmacology*; D013499:Surface Properties / Q000187:drug effects; D013997:Time Factors; D055454:Trametes / Q000201:enzymology* / Q000235:genetics / Q000302:isolation & purification; D001617:beta-Glucosidase / Q000378:metabolism
D012972:Sodium Hydroxide; D002244:Carbon; D001617:beta-Glucosidase; D002480:Cellulase; D043366:Cellulose 1,4-beta-Cellobiosidase; D009584:Nitrogen