Anti-obesity effect of sulfated glucosamine by AMPK signal pathway in 3T3-L1 adipocytes.
In this study, we investigated the effect of sulfated glucosamine (SGlc) on adipogenesis of 3T3-L1 adipocytes during differentiation of preadipocytes into adipocytes by measuring lipid accumulation and adipogenesis related factors. Treatment with SGlc reduced the triglyceride content in Oil-Red O staining and enhanced glycerol secretion in adipocytes in a dose-dependent manner. In addition, SGlc induced the down-regulation of adipogenesis related factors and adipocyte specific gene promoters. Moreover, treatment of 3T3-L1 adipocytes with SGlc activated the phosphorylated adenosine monophosphate-activated protein kinase (AMPK) alpha and beta along with their substrate, acetyl-CoA carboxylase (ACC). These results suggest that inhibitory effect of SGlc on adipocyte differentiation might be mediated through the up-regulation of AMPK pathway.
Kong
Chang-Suk
CS
Marine Bioprocess Research Center, Pukyong National University, Busan 608-737, Republic of Korea.
Kim
Jung-Ae
JA
Kim
Se-Kwon
SK
D016475:3T3 Cells / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D055372:AMP-Activated Protein Kinases / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D000103:Acetyl-CoA Carboxylase / Q000235:genetics / Q000378:metabolism; D017667:Adipocytes / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D050156:Adipogenesis / Q000187:drug effects* / Q000235:genetics; D000818:Animals; D019440:Anti-Obesity Agents / Q000494:pharmacology*; D002352:Carrier Proteins; D002454:Cell Differentiation / Q000187:drug effects; D005786:Gene Expression Regulation / Q000187:drug effects; D005944:Glucosamine / Q000031:analogs & derivatives* / Q000494:pharmacology; D005990:Glycerol / Q000378:metabolism; D050356:Lipid Metabolism / Q000187:drug effects; D051379:Mice; D000070778:Perilipin-1; D010750:Phosphoproteins / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics; D015398:Signal Transduction / Q000187:drug effects; D002787:Sterol Esterase / Q000235:genetics / Q000378:metabolism; D014280:Triglycerides / Q000378:metabolism
D019440:Anti-Obesity Agents; D002352:Carrier Proteins; D000070778:Perilipin-1; D010750:Phosphoproteins; D012333:RNA, Messenger; D014280:Triglycerides; C046905:glucosamine 6-O-sulfate; D055372:AMP-Activated Protein Kinases; D002787:Sterol Esterase; D000103:Acetyl-CoA Carboxylase; D005944:Glucosamine; D005990:Glycerol