[Association between Fas/Fas L genes promoter polymorphisms and pathogenic risk of cervical cancer].
OBJECTIVE
To investigate the association between apoptosis genes Fas/Fas L promoter polymorphisms and the risk of the development of cervical cancer.


METHODS
Blood samples were collected from 314 cases with primary cervical cancer and 615 healthy controls. Genotypes of Fas/Fas L genes were determined by polymerase chain reaction-based restriction fragment length polymorphism. The associations with the risk of cervical cancer and impact of clinicopathological characteristics were estimated by logistic regression.


RESULTS
Fas L-844CC genotype was significantly associated with increased risk of cervical cancer compared with Fas L-844TC or -TT genotype (OR = 3.05; P < 0.01). However, there was no significant difference of Fas-670A/G or -1377G/A genotypes. Interaction of genetic polymorphism between Fas and Fas L was observed. Stratification analysis revealed that Fas-670G or -1377A allele was significantly higher in squamous carcinoma in situ (OR = 1.77 or 1.93; P < 0.05) while Fas L-844CC genotype had an increased risk of invasive squamous carcinoma compared with that of Fas L-844TT genotype (OR = 3.33; P < 0.01). No significant associations were observed between polymorphisms in Fas/Fas L and clinical FIGO stage, cell differentiation, size of tumors, serum squamous cell carcinoma antigen value at the diagnosis and so on.


CONCLUSIONS
The results of this study suggest that genetic polymorphisms of Fas and Fas L in apoptotic pathway are associated with the risk of development of cervical carcinoma.
Li
Hua
H
Department of Obstetrics and Gynecology, Third Hospital, Peking University, Beijing 100083, China. huali88@sina.com
Guo
Hong-yan
HY
Sun
Tong
T
Zhou
Yi-feng
YF
Lin
Dong-xin
DX
Zhang
Wen-hua
WH
Qiao
Jie
J
D000328:Adult; D000483:Alleles; D002278:Carcinoma in Situ / Q000235:genetics; D002294:Carcinoma, Squamous Cell / Q000235:genetics*; D053222:Fas Ligand Protein / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D016015:Logistic Models; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D018570:Risk Assessment; D012307:Risk Factors; D002583:Uterine Cervical Neoplasms / Q000235:genetics*; D019014:fas Receptor / Q000235:genetics*
C491901:FAS protein, human; D053222:Fas Ligand Protein; D019014:fas Receptor