Estimation of peptide concentration by a modified bicinchoninic acid assay.
Although biuret based protein assays are theoretically applicable to peptide measurement, there is a high level of interpeptide variation, determined largely by peptide hydrophobicity. This variation in peptide reactivity can be significantly reduced by heat-denaturation of peptides at 95 degrees C for 5 min in the presence of 0.1 M NaOH containing 1% (w/v) SDS, prior to incubation for 30 min at 37 degrees C in BCA standard working reagent. This modification to the standard bicinchoninic acid (BCA) assay protocol allows for an accurate, rapid, and economical estimation of the peptide concentration within an unknown sample.
Kapoor
Krishan N
KN
School of Science and Technology, Nottingham Trent University, Nottingham NG11 8NS, UK.
Barry
Danielle T
DT
Rees
Robert C
RC
Dodi
I Anthony
IA
McArdle
Stephanie E B
SE
Creaser
Colin S
CS
Bonner
Philip L R
PL
D000818:Animals; D002460:Cell Line; D006168:Guinea Pigs; D010455:Peptides / Q000032:analysis*; D011804:Quinolines / Q000737:chemistry*
D010455:Peptides; D011804:Quinolines; C047117:bicinchoninic acid