Teucrium polium complex with molybdate enhance cultured islets secretory function.
Islet transplantation has become a promising treatment in the therapy of type 1 diabetes. Its function improvement, after isolation and before transplantation, is crucial because of their loss both in number and function of islets after isolation procedures. Trace elements sodium orthovanadate (SOV) and sodium molybdate (SM), as well as medicinal plant Teucrium polium L. (TP), showed and possessed high beneficial antioxidative potential and even hypoglycemic properties via their effect on islets. We evaluated the effect of these components in combination on cultured islet function in order to improve pancreatic islet transplantation. Rat pancreatic islets were cultured for 24 h then incubated with different concentrations of TP (0.01 and 0.1 mg/mL) alone and in combination with SOV (1 mM) or SM (1 mM). Insulin concentration in buffer media was measured as islet secretory function. Administration of TP (0.01 mg/mL), SM, and SOV alone or in combination with each other significantly increased insulin secretion at high glucose concentration (16.7 mM); insulin secretion was significantly greater in the group containing both TP and SM than other treated groups (p < 0.05). The combination of the mentioned trace elements especially molybdate with TP could improve islet cells function before transplantation.
Mohseni Salehi Monfared
Seyed Sajad
SS
Endocrinology and Metabolism Research Center, Tehran University of Medical Sciences, Tehran, Iran. sajad.salehi@gmail.com
Pournourmohammadi
Shirin
S
D000818:Animals; D002470:Cell Survival; D002478:Cells, Cultured; D003922:Diabetes Mellitus, Type 1 / Q000601:surgery; D007328:Insulin / Q000378:metabolism; D000078790:Insulin Secretion; D007515:Islets of Langerhans / Q000187:drug effects* / Q000378:metabolism; D016381:Islets of Langerhans Transplantation; D008297:Male; D008982:Molybdenum / Q000737:chemistry / Q000494:pharmacology; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D031367:Teucrium / Q000737:chemistry*
D007328:Insulin; D010936:Plant Extracts; C044659:molybdate; D008982:Molybdenum