Expression of angiotensin II type 1 (AT1) and angiotensin II type 2 (AT2) receptors in human granulosa-lutein (GL) cells: correlation with infertility diagnoses.
OBJECTIVE
To correlate angiotensin II (AngII) receptor expression by granulosa-lutein (GL) cells from gonadotropin-stimulated follicles with infertility diagnosis and IVF parameters.


METHODS
The mRNA of angiotensin receptors type 1 (AT1) and type 2 (AT2) was studied in aspirated GL cells.


METHODS
University laboratory and private IVF center.


METHODS
Seventy-three IVF patients.


METHODS
Reverse-transcription polymerase chain reaction analysis for relative expression of AT1 and AT2 receptor mRNA in women with no ovarian factor (NOF), poor ovarian reserve (PR), endometriosis (ENDO), and polycystic ovary syndrome (PCOS).


METHODS
Expression of AT1 and AT2 receptor mRNA.


RESULTS
There was a constant approximately 7:1 ratio between AT1 and AT2 receptors and a negative correlation between the AT1/AT2 ratio and patient age. There were statistically significant differences in AngII receptors in individual conditions: NOF showed a correlation between AT1 and AT2 receptors and a negative correlation between AT1 receptor expression, embryo fragmentation and number of metaphase II (MII) oocytes; PR showed a negative correlation between AT2 receptor expression and number of MII oocytes; PCOS AT1 receptor expression correlated negatively with the units of FSH administered and with patients' age; ENDO showed no significant correlations.


CONCLUSIONS
Mural GL cells express AT1 receptor much more than AT2 receptor. AngII receptor expression varies with age and infertility diagnosis. Low expression of AngII receptors was associated with high-dose stimulation in women with PR. Embryo fragmentation in NOF is associated with decreased AT1 receptor expression, supporting a role for AngII in GL cell apoptosis.
Peña
Oscar
O
Laboratorio de Biología del Desarrollo, Departamento de Bioquímica y Biología Molecular, Universidad de La Laguna, La Laguna, Tenerife, Spain.
Palumbo
Angela
A
González-Fernández
Rebeca
R
Hernández
Jairo
J
Naftolin
Frederick
F
Avila
Julio
J
D000328:Adult; D000367:Age Factors; D017209:Apoptosis; D046149:Embryo Culture Techniques; D020964:Embryo Loss; D004715:Endometriosis / Q000175:diagnosis / Q000235:genetics* / Q000628:therapy; D005260:Female; D015571:Follicular Fluid / Q000166:cytology; D018507:Gene Expression Regulation, Developmental; D006801:Humans; D007247:Infertility, Female / Q000175:diagnosis / Q000235:genetics* / Q000628:therapy; D008184:Luteal Cells / Q000378:metabolism* / Q000473:pathology; D008677:Metaphase; D011085:Polycystic Ovary Syndrome / Q000175:diagnosis / Q000235:genetics* / Q000628:therapy; D012333:RNA, Messenger / Q000032:analysis*; D044140:Receptor, Angiotensin, Type 1 / Q000235:genetics* / Q000378:metabolism; D044139:Receptor, Angiotensin, Type 2 / Q000235:genetics* / Q000378:metabolism; D027724:Reproductive Techniques, Assisted; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013997:Time Factors
C544065:AGTR1 protein, human; D012333:RNA, Messenger; D044140:Receptor, Angiotensin, Type 1; D044139:Receptor, Angiotensin, Type 2