title: Antipyretic and anti-inflammatory properties of the ethanolic extract, dichloromethane fraction and costunolide from Magnolia ovata (Magnoliaceae).
abstract: OBJECTIVE
  Magnolia ovata (A.St.-Hil.) Spreng (formerly Talauma ovata), known as "pinha-do-brejo" or "baguaçu", is a large tree widely distributed in Brazil. Its trunk bark has been used in folk medicine against fever. However, no data have been published to support the antipyretic ethnopharmacological use. This study investigated the antipyretic and anti-inflammatory effects of the ethanolic extract (EEMO), dichloromethane fraction (DCM), and the isolated compound costunolide.
  
  
  METHODS
  The antipyretic and anti-inflammatory activities were evaluated in experimental models of fever and inflammation in mice.
  
  
  RESULTS
  The oral administration of EEMO, DCM and costunolide inhibited carrageenan (Cg)-induced paw oedema (ID(50) 72.35 (38.64-135.46) mg/kg, 5.8 (2.41-14.04) mg/kg and 0.18 (0.12-0.27) mg/kg, respectively) and was effective in abolishing lipopolysaccharide (LPS)-induced fever (30 mg/kg, 4.5 mg/kg and 0.15 mg/kg, respectively). EEMO was also effective in reducing cell migration in the pleurisy model. Intraplantar injection of costunolide also reduced the paw oedema, myeloperoxidase and N-acetyl-glucosaminidase activity induced by Cg in mice.
  
  
  CONCLUSIONS
  Collectively, these results show, for the first time, that extracts obtained from Magnolia ovata possess antipyretic and anti-inflammatory properties, and costunolide appears to be the compound responsible for these effects.
authors.affiliation: Department of Pharmacology, Universidade Federal do Paraná, Curitiba, PR, Brazil.
authors.forename: Cândida Aparecida Leite
authors.initials: CA
authors.lastname: Kassuya
authors.forename: Aline
authors.initials: A
authors.lastname: Cremoneze
authors.forename: Letícia Ferrari Lemos
authors.initials: LF
authors.lastname: Barros
authors.forename: Alex Sandro
authors.initials: AS
authors.lastname: Simas
authors.forename: Fernanda da Rocha
authors.initials: Fda R
authors.lastname: Lapa
authors.forename: Renato
authors.initials: R
authors.lastname: Mello-Silva
authors.forename: Maria Elida Alves
authors.initials: ME
authors.lastname: Stefanello
authors.forename: Aleksander Roberto
authors.initials: AR
authors.lastname: Zampronio
mesh_terms: D000118:Acetylglucosaminidase / Q000378:metabolism; D018712:Analgesics, Non-Narcotic / Q000494:pharmacology*; D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology*; D002351:Carrageenan; D002465:Cell Movement / Q000187:drug effects; D004487:Edema / Q000139:chemically induced / Q000517:prevention & control; D000431:Ethanol; D005122:Exudates and Transudates / Q000378:metabolism; D005334:Fever / Q000139:chemically induced / Q000517:prevention & control; D007202:Indicators and Reagents; D007962:Leukocytes / Q000187:drug effects; D008070:Lipopolysaccharides; D031566:Magnolia / Q000737:chemistry*; D008297:Male; D008752:Methylene Chloride; D051379:Mice; D009195:Peroxidase / Q000378:metabolism; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology; D035422:Pleural Cavity / Q000166:cytology; D012717:Sesquiterpenes / Q000737:chemistry / Q000494:pharmacology*; D012997:Solvents; D007783:Lactones
chemical_list: D000118:Acetylglucosaminidase; D018712:Analgesics, Non-Narcotic; D000894:Anti-Inflammatory Agents, Non-Steroidal; D002351:Carrageenan; D000431:Ethanol; D007202:Indicators and Reagents; D008070:Lipopolysaccharides; D008752:Methylene Chloride; D009195:Peroxidase; D010936:Plant Extracts; D012717:Sesquiterpenes; D012997:Solvents; C002602:costunolide; D007783:Lactones