title: Expression of bone morphogenetic proteins in giant cell tumor of bone.
abstract: BACKGROUND
  A giant cell tumor (GCT) of bone is a locally aggressive tumor with a propensity for local recurrence. A characteristic pattern of peripheral bone formation has been described in GCT recurrence in soft tissue, and in some pulmonary metastases from benign GCT. Although the bone formation in GCT in supposedly due to bone morphogenetic proteins (BMPs), the expression pattern of BMPs in GCT has not been well investigated.
  
  
  METHODS
  The expression of BMPs in GCT tissues, cultured stromal cells from GCT, and osteoclast-like giant cells harvested by laser microdissection (LM), as well as from control osteosarcoma (NOS-1) cells was analyzed using reverse transcriptional-semiquantitative PCR.
  
  
  RESULTS
  BMP 2, 3, 4, 5 and 6 were expressed in the GCT tissue. The cultured GCT cells expressed BMP 2, 4, 5 and 6. The osteoclast-like giant cells expressed BMP 2, 3, 5 and 6 and BMP 5 was expressed at the highest level.
  
  
  CONCLUSIONS
  Both stromal cells and osteoclast-like cells in GCT expressed several kinds of BMPs.
authors.affiliation: Division of Orthopedic Surgery, Department of Regenerative Transplant Medicine, Niigata University Graduate School of Medical and Dental Sciences, Niigata City, Niigata 951-8510, Japan.
authors.forename: Naoko
authors.initials: N
authors.lastname: Kudo
authors.forename: Akira
authors.initials: A
authors.lastname: Ogose
authors.forename: Takashi
authors.initials: T
authors.lastname: Ariizumi
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Kawashima
authors.forename: Tetsuo
authors.initials: T
authors.lastname: Hotta
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Hatano
authors.forename: Tetsuro
authors.initials: T
authors.lastname: Morita
authors.forename: Masaki
authors.initials: M
authors.lastname: Nagata
authors.forename: Yukie
authors.initials: Y
authors.lastname: Siki
authors.forename: Akira
authors.initials: A
authors.lastname: Kawai
authors.forename: Yuko
authors.initials: Y
authors.lastname: Hotta
authors.forename: Makiko
authors.initials: M
authors.lastname: Hoshino
authors.forename: Naoto
authors.initials: N
authors.lastname: Endo
mesh_terms: D000328:Adult; D019485:Bone Morphogenetic Proteins / Q000235:genetics* / Q000378:metabolism; D001859:Bone Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D005260:Female; D018212:Giant Cell Tumor of Bone / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D006801:Humans; D007834:Lasers; D008297:Male; D042282:Microdissection; D008875:Middle Aged; D012516:Osteosarcoma / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017154:Stromal Cells / Q000378:metabolism / Q000473:pathology; D014407:Tumor Cells, Cultured; D055815:Young Adult
chemical_list: D019485:Bone Morphogenetic Proteins; D012333:RNA, Messenger