title: Formation of DNA toroids inside confined droplets adsorbed on mica surfaces.
abstract: We report observations of in vitro DNA compaction into toroids in the absence of any condensing agent. The DNA toroid formation is induced by geometry confinement from microdroplets on mica surfaces. With AFM imaging we show that the confined DNA molecules may take the form of random coils or semiordered folded loops with large microdroplets, while they readily take the form of compact and ordered toroids when the microdroplet sizes are small enough. To better understand these phenomena, we carried out coarse-grained Brownian dynamics simulation, obtaining results that were in good agreement with the experimental observations. The simulation reveals that the toroid formation is sensitive to not only the microdroplet size, but also the DNA stiffness.
authors.affiliation: Laboratory of Soft Matter Physics, Beijing National Laboratory for Condensed Matter Physics, Institute of Physics, Chinese Academy of Sciences, Beijing 100190, China.
authors.forename: Xi-Miao
authors.initials: XM
authors.lastname: Hou
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.forename: Shuo-Xing
authors.initials: SX
authors.lastname: Dou
authors.forename: Ling-Yun
authors.initials: LY
authors.lastname: Zhang
authors.forename: Ping
authors.initials: P
authors.lastname: Xie
authors.forename: Wei-Chi
authors.initials: WC
authors.lastname: Wang
authors.forename: Peng-Ye
authors.initials: PY
authors.lastname: Wang
mesh_terms: D000538:Aluminum Silicates / Q000737:chemistry*; D003198:Computer Simulation; D004247:DNA / Q000737:chemistry* / Q000648:ultrastructure*; D008956:Models, Chemical; D008958:Models, Molecular; D009690:Nucleic Acid Conformation; D012996:Solutions; D013499:Surface Properties
chemical_list: D000538:Aluminum Silicates; D012996:Solutions; D004247:DNA; C011934:mica