title: Oxidative modification of fibrinogen inhibits its transformation into fibrin under the effect of thrombin.
abstract: Changes in the capacity of fibrinogen subjected to oxidative modification to transform into fibrin under the effect of thrombin and to form a fibrin clot were studied. The effects of oxidized fibrinogen preparations on the clot formation by citrate-treated donor plasma were evaluated by the thrombin time test. Oxidation impaired the capacity of isolated fibrinogen to form a fibrin clot under the effect of thrombin. Addition of oxidized fibrinogen solutions to donor plasma led to prolongation of the plasma clotting time. Maximum addition (33% volume) of oxidized fibrinogen led to a 10-26% prolongation of clotting time in comparison with addition of the same volume of the same solution without fibrinogen.
authors.affiliation: Laboratory of Biophysical Bases of Pathology, Institute of Physico-Chemical Medicine, Federal Agency for Public Health and Social Development, Moscow, Russia. o.azizova@mail.ru
authors.forename: O A
authors.initials: OA
authors.lastname: Azizova
authors.forename: A P
authors.initials: AP
authors.lastname: Piryazev
authors.forename: A V
authors.initials: AV
authors.lastname: Aseychev
authors.forename: A G
authors.initials: AG
authors.lastname: Shvachko
mesh_terms: D005337:Fibrin / Q000737:chemistry* / Q000378:metabolism; D005340:Fibrinogen / Q000737:chemistry* / Q000378:metabolism; D006801:Humans; D010084:Oxidation-Reduction; D018384:Oxidative Stress / Q000502:physiology; D013917:Thrombin / Q000737:chemistry* / Q000378:metabolism; D013918:Thrombin Time
chemical_list: D005337:Fibrin; D005340:Fibrinogen; D013917:Thrombin