Rep1p negatively regulating MDR1 efflux pump involved in drug resistance in Candida albicans.
Overexpression of MDR1 efflux pump is a major mechanism contributing to drug resistance in Candida albicans, the most common human fungal pathogen. To elucidate the regulatory pathway of drug resistance, we have identified a negative regulator of MDR1 and named it Regulator of Efflux Pump 1 (REP1). Overexpression of REP1 in Saccharomyces cerevisiae increased susceptibility to fluconazole. Furthermore, null mutations on REP1 decreased the susceptibility to antifungal drugs in C. albicans resulting from increased expression of MDR1 mRNA. Hence, Rep1p is involved in drug resistance by negatively regulating MDR1 in C. albicans.
Chen
Chia-Geun
CG
Institute of Preventive Medicine, National Defense Medical Center, Taiwan, ROC.
Yang
Yun-Liang
YL
Tseng
Kuo-Yun
KY
Shih
Hsin-I
HI
Liou
Ci-Hong
CH
Lin
Chih-Chao
CC
Lo
Hsiu-Jung
HJ
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1 / Q000235:genetics / Q000378:metabolism*; D000595:Amino Acid Sequence; D000935:Antifungal Agents / Q000494:pharmacology; D001483:Base Sequence; D002176:Candida albicans / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism*; D002177:Candidiasis / Q000188:drug therapy / Q000378:metabolism / Q000382:microbiology*; D003001:Cloning, Molecular; D025141:Drug Resistance, Fungal / Q000235:genetics*; D015725:Fluconazole / Q000494:pharmacology; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism*; D017353:Gene Deletion; D015966:Gene Expression Regulation, Fungal; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D012331:RNA, Fungal / Q000032:analysis / Q000096:biosynthesis / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis / Q000096:biosynthesis / Q000235:genetics
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D000935:Antifungal Agents; D005656:Fungal Proteins; D012331:RNA, Fungal; D012333:RNA, Messenger; D015725:Fluconazole