Evaluation of new preanalysis sample treatment tools and DNA isolation protocols to improve bacterial pathogen detection in whole blood.
Two novel preanalysis sample treatment tools were evaluated in combination with four DNA extraction kits for the selective isolation of bacterial DNA from whole blood. The combination of performing a preanalysis sample treatment and using a larger sample volume increased the detection limit to 50 CFU per ml.
Hansen
Wendy L J
WL
Department of Medical Microbiology, Maastricht University Medical Center, The Netherlands.
Bruggeman
Cathrien A
CA
Wolffs
Petra F G
PF
D001419:Bacteria / Q000235:genetics / Q000302:isolation & purification*; D001769:Blood / Q000382:microbiology*; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D011933:Reagent Kits, Diagnostic; D012680:Sensitivity and Specificity; D013048:Specimen Handling / Q000379:methods*
D004269:DNA, Bacterial; D011933:Reagent Kits, Diagnostic