title: The infectious molecular clone and pseudotyped virus models of human immunodeficiency virus type 1 exhibit significant differences in virion composition with only moderate differences in infectivity and inhibition sensitivity.
abstract: Two frequently employed methods for generating well-characterized, genetically defined infectious human immunodeficiency virus type 1 in vitro include the use of infectious molecular clones (IMCs) and pseudoviruses (PVs) competent for single-round infection. We compared six matched pairs of IMCs and PVs. The relative amounts of Env incorporated and efficiency of cleavage differed substantially between the two systems. Altering the ratio of proviral genome and env expression plasmids can produce pseudovirions that are structurally more similar to the matched IMCs. Differences in Env incorporation and cleavage translated into moderate differences in assays infectivity and sensitivity to neutralizing antibodies and entry inhibitors.
authors.affiliation: Department of Microbiology, University of Washington, Seattle, Washington 98195, USA.
authors.forename: Nicholas M
authors.initials: NM
authors.lastname: Provine
authors.forename: Wendy Blay
authors.initials: WB
authors.lastname: Puryear
authors.forename: Xueling
authors.initials: X
authors.lastname: Wu
authors.forename: Julie
authors.initials: J
authors.lastname: Overbaugh
authors.forename: Nancy L
authors.initials: NL
authors.lastname: Haigwood
mesh_terms: D000914:Antibodies, Viral / Q000276:immunology*; D002460:Cell Line; D023581:HIV Fusion Inhibitors / Q000494:pharmacology*; D015497:HIV-1 / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development* / Q000276:immunology; D006801:Humans; D014771:Virion / Q000737:chemistry*; D054299:env Gene Products, Human Immunodeficiency Virus / Q000032:analysis*
chemical_list: D000914:Antibodies, Viral; D023581:HIV Fusion Inhibitors; D054299:env Gene Products, Human Immunodeficiency Virus