High circulating autoantibodies against human oxidized low-density lipoprotein are related to stable and lower titers to unstable clinical situation.
BACKGROUND
Oxidized lipoproteins and antibodies anti-oxidized low-density lipoprotein (anti-oxLDL) have been detected in human plasma and in atherosclerotic lesions. However, the role of these autoantibodies in the maintenance of vascular health or in the pathogenesis of acute vascular insults remains unclear. We examined the relationship of human immunoglobulin G (IgG) anti-oxLDL antibodies with cardiovascular disease risk markers in stable subjects and in patients after an acute coronary syndrome (ACS).


METHODS
Titers of human anti-oxLDL antibodies were measured in hypertensive subjects in primary prevention (n=94), without other risk factors, and in individuals after a recent ACS event who also had metabolic syndrome (n=116). Autoantibodies against copper ion oxidized LDL were measured by enzyme-linked-immunosorbent assay.


RESULTS
Anti-oxLDL titers were higher in hypertensive patients and these subjects presented lower high sensitivity C-reactive protein (hs-CRP) than those with ACS (p<0.0001). We found significant correlations between anti-oxLDL and hs-CRP (r=-0.284), body mass index (r=-0.256), waist circumference (r=-0.368), apolipoprotein B (r=-0.191), glucose (r=-0.303), systolic blood pressure (r=0.319), diastolic blood pressure (r=0.167), high-density lipoprotein cholesterol (r=0.224) and apolipoprotein A1 (r=0.257) (p<0.02 for all). After multiple linear regression hs-CRP, fasting glucose and waist circumference remained independently and inversely associated with anti-oxLDL.


CONCLUSIONS
Acute inflammatory and metabolic conditions decrease titers of human antibodies of IgG class against oxidized LDL, and that circulating anti-oxLDL antibodies could be associated with a protective role in atherosclerosis.
Santos
Andreza O
AO
Cardiology Division, Department of Medicine, Federal University of Sao Paulo, Sao Paulo, SP, Brazil.
Fonseca
Francisco A H
FA
Fischer
Simone M
SM
Monteiro
Carlos M C
CM
Brandão
Sergio A B
SA
Póvoa
Rui M S
RM
Bombig
Maria T N
MT
Carvalho
Antonio C
AC
Monteiro
Andréa M
AM
Ramos
Eduardo
E
Gidlund
Magnus
M
Figueiredo Neto
Antonio M
AM
Izar
Maria C O
MC
D054058:Acute Coronary Syndrome / Q000097:blood / Q000150:complications / Q000276:immunology; D000328:Adult; D000368:Aged; D000704:Analysis of Variance; D001323:Autoantibodies / Q000097:blood* / Q000276:immunology*; D015415:Biomarkers / Q000097:blood; D002097:C-Reactive Protein / Q000378:metabolism; D005260:Female; D006801:Humans; D006973:Hypertension / Q000097:blood / Q000150:complications / Q000276:immunology; D016014:Linear Models; D008077:Lipoproteins, LDL / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D012307:Risk Factors
D001323:Autoantibodies; D015415:Biomarkers; D008077:Lipoproteins, LDL; C099252:oxidized low density lipoprotein; D002097:C-Reactive Protein