Identification of FAM46D as a novel cancer/testis antigen using EST data and serological analysis.
Cancer/testis Antigens (CTAs) are immunogenic proteins with a restricted expression pattern in normal tissues and aberrant expression in different types of tumors being considered promising candidates for immunotherapy. We used the alignment between EST sequences and the human genome sequence to identify novel CT genes. By examining the EST tissue composition of known CT clusters we defined parameters for the selection of 1184 EST clusters corresponding to putative CT genes. The expression pattern of 70 CT gene candidates was evaluated by RT-PCR in 21 normal tissues, 17 tumor cell lines and 160 primary tumors. We were able to identify 4 CT genes expressed in different types of tumors. The presence of antibodies against the protein encoded by 1 of these 4 CT genes (FAM46D) was exclusively detected in plasma samples from cancer patients. Due to its restricted expression pattern and immunogenicity FAM46D represents a novel target for cancer immunotherapy.
Bettoni
Fabiana
F
Ludwig Institute for Cancer Research, Hospital Alemão Oswaldo Cruz, São Paulo, SP, Brazil.
Filho
Fernando Camargo
FC
Grosso
Daniela M
DM
Galante
Pedro A F
PA
Parmigiani
Raphael B
RB
Geraldo
Murilo V
MV
Henrique-Silva
Flávio
F
Oba-Shinjo
Sueli M
SM
Marie
Suely K N
SK
Soares
Fernando A
FA
Brentani
Helena P
HP
Simpson
Andrew J G
AJ
de Souza
Sandro J
SJ
Camargo
Anamaria A
AA
D000951:Antigens, Neoplasm / Q000235:genetics / Q000276:immunology*; D016022:Case-Control Studies; D030561:Databases, Nucleic Acid; D020224:Expressed Sequence Tags; D015894:Genome, Human; D006801:Humans; D008297:Male; D009363:Neoplasm Proteins / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D009369:Neoplasms / Q000097:blood* / Q000473:pathology; D009713:Nucleotidyltransferases; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology; D013737:Testis / Q000276:immunology; D014407:Tumor Cells, Cultured
D000951:Antigens, Neoplasm; D009363:Neoplasm Proteins; D011994:Recombinant Proteins; D009713:Nucleotidyltransferases; C545355:TENT5D protein, human