Effects of three diterpenoids on tumour cell proliferation and telomerase activity.
Xerophilusin B (XB), macrocalin B (MB) and eriocalyxin B (EB) belong to ent-kaurene diterpenoids. The inhibition effects of three ent-kaurane compounds on the proliferation of six tumour lines (K562, HL-60, A549, MKN, HCT and CA) were estimated by methyl-thiazol-tetrazolium, and the telomerase activity in K562 cells was measured by the telomeric repeat amplification protocol-enzyme-linked immunosorbent assay. We found that XB, MB and EB could significantly inhibit the proliferation of tumour cells. Within the concentration range of 10(-4)-10(-8) mol L(-1), three ent-kaurane compounds could markedly inhibit the telomerase activity in K562 cells, and their effects on telomerase were exhibited in a dose-dependent manner. Our results demonstrate that XB, MB and EB can inhibit the proliferation of tumour cells and the telomerase activity, which provides data for developing ent-compounds as antitumour drugs.
Yang
Yunshan
Y
Yunnan Pharmacological Laboratory for Natural Products, Kunming Medical College, Kunming, PR China.
Sun
Handong
H
Zhou
Yiping
Y
Ji
Shuyu
S
Li
Malin
M
D049109:Cell Proliferation / Q000187:drug effects*; D004224:Diterpenes / Q000737:chemistry / Q000494:pharmacology*; D006801:Humans; D037602:Isodon / Q000737:chemistry; D020014:K562 Cells; D015394:Molecular Structure; D019098:Telomerase / Q000187:drug effects*
D004224:Diterpenes; C516552:eriocalyxin B; C410911:xerophilusin B; C045862:macrocalin B; D019098:Telomerase