title: Electrospray ionization mass spectrometric observation of ligand exchange of zinc pyrithione with amino acids.
abstract: Zinc pyrithione (ZnPT) is widely used as an antidandruff or antifouling reagent. However, this compound is considered toxic, such as the teratogenic effect, to aquatic lives, and it is important to clarify the mechanism of its toxicity. In this study, the interactions between ZnPT and amino acids were observed using electrospray ionization mass spectrometry (ESI-MS) in order to obtain information on the activity of ZnPT within the living body. The ZnPT complex ([ZnPT-ligand+Amino acid]+), in which the ligand of ZnPT was exchanged by the amino acid, was detected in ZnPT solutions mixed with one of 20 amino acids by ESI-MS. Histidine and cysteine, in particular, showed a high reactivity with ZnPT, while serine and glycine showed a low reactivity. The complexes of ZnPT and a peptide were also observed by the ESI-MS measurement of the solution containing ZnPT with the peptide. These results would be useful to understand the mechanism of ZnPT toxicities to living creatures.
authors.affiliation: Shinshu University, Faculty of Textile Science and Technology, Division of Applied Biology, 3-15-1, Tokida, Ueda 386-8567, Japan. moriwaki@shinshu-u.ac.jp
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Moriwaki
authors.forename: Masanori
authors.initials: M
authors.lastname: Okabayashi
authors.forename: Takehiro
authors.initials: T
authors.lastname: Watanabe
authors.forename: Hideya
authors.initials: H
authors.lastname: Kawasaki
authors.forename: Ryuichi
authors.initials: R
authors.lastname: Arakawa
mesh_terms: D000596:Amino Acids / Q000737:chemistry*; D008024:Ligands; D009942:Organometallic Compounds / Q000737:chemistry* / Q000633:toxicity; D011725:Pyridines / Q000737:chemistry* / Q000633:toxicity; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
chemical_list: D000596:Amino Acids; D008024:Ligands; D009942:Organometallic Compounds; D011725:Pyridines; C010423:pyrithione zinc