title: [Simultaneous determination of four alkaloids in Lindera aggregate by high performance liquid chromatography].
abstract: OBJECTIVE
  To develop an HPLC method for simultaneous determination of four major alkaloids in Lindera aggregate.
  
  
  METHODS
  The analysis was carried out on an Agilent ZORBAX SB-C18 column (4.6 mm x 250 mm, 5 microm) with gradient elution using acetonitrile-water (containing 0. 15% diethylamine, adjusted to pH = 3.0 with acetic acid) as mobile phase. Flow rate was 1.0 mL x min(-1) and the detection wavelength was at 289 nm.
  
  
  RESULTS
  The calibration curves were linear over the range of 0.428-8.560 microg for boldine, 2.122-31.83 microg for norboldine, 0.760-15.20 microg for reticuline and 0.020 4-0.400 8 microg for linderegatine, respectively. The average recoveries were 99.18% for boldine, 101.0% for norboldine, 100.3% for reticuline and 99.17% for linderegatine, respectively. with RSD not more than 3.0%.
  
  
  CONCLUSIONS
  The described method is reliable and convenient and could be used for the quality control of Lindera aggregate.
authors.affiliation: Institute of Modern Chinese Medicine, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058, China.
authors.forename: Zheng
authors.initials: Z
authors.lastname: Han
authors.forename: Huili
authors.initials: H
authors.lastname: Su
authors.forename: Na
authors.initials: N
authors.lastname: Chen
authors.forename: Lianjun
authors.initials: L
authors.lastname: Luan
authors.forename: Yongjiang
authors.initials: Y
authors.lastname: Wu
mesh_terms: D000470:Alkaloids / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D027443:Lindera / Q000737:chemistry*
chemical_list: D000470:Alkaloids