title: Characterization and antineoplasic effect of extracts obtained from Pleurotus sajor-caju fruiting bodies.
abstract: Fungi of the Pleurotus genus present a great industrial interest due to their possibility of producing pharmacological compounds, pigments, aromas, organic acids, polysaccharides, enzymes, vitamins, amino acids, etc. Among the therapeutic products, we can highlight those with antineoplasic activity, attributed to the fungi cell wall components. Based on this, the objective of this work was to study the antineoplasic capacity of the polysaccharidic fractions obtained from Pleurotus sajor-caju fruiting bodies. Female Swiss mice were inoculated with the Ehrlich ascitic tumor (5 x 10(6) cells/animal) in ascitic form. The polysaccharidic fractions were administered intraperitoneally, during a 6-day period. Fractions FI and FII presented a lower volume of ascitic liquid (3.1 and 1.8 mL, respectively) and a higher reduction in the number of neoplasic cells present in the ascitic liquid (86.2% and 85%, respectively), when compared to the positive control (group inoculated with the tumor but without treatment). These fractions were characterized in terms of monosaccharide composition. Glucose was the major component detected, followed by galactose and mannose. The anomeric carbon configuration of the beta-glucan was confirmed by the (13)C NMR (delta 103.7). Substituted and free C3 and C6 were also detected. Protein bands were confirmed through infrared analysis.
authors.affiliation: University of the Region of Joinville-UNIVILLE, Campus Universitário, s/n Bom Retiro, 89201-974, Joinville, Santa Catarina, Brazil.
authors.forename: Nicole
authors.initials: N
authors.lastname: Dalonso
authors.forename: Rafaela
authors.initials: R
authors.lastname: Souza
authors.forename: Márcia Luciane Lange
authors.initials: ML
authors.lastname: Silveira
authors.forename: Angelo Adolfo
authors.initials: AA
authors.lastname: Ruzza
authors.forename: Theodoro Marcel
authors.initials: TM
authors.lastname: Wagner
authors.forename: Elisabeth
authors.initials: E
authors.lastname: Wisbeck
authors.forename: Sandra Aparecida
authors.initials: SA
authors.lastname: Furlan
mesh_terms: D000328:Adult; D000818:Animals; D000970:Antineoplastic Agents / Q000737:chemistry / Q000627:therapeutic use*; D001835:Body Weight; D002286:Carcinoma, Ehrlich Tumor / Q000188:drug therapy* / Q000473:pathology; D002457:Cell Extracts / Q000737:chemistry / Q000627:therapeutic use*; D002648:Child; D005260:Female; D048690:Fruiting Bodies, Fungal / Q000737:chemistry*; D006801:Humans; D051379:Mice; D009368:Neoplasm Transplantation; D019906:Nuclear Magnetic Resonance, Biomolecular; D020076:Pleurotus / Q000737:chemistry / Q000166:cytology; D011134:Polysaccharides / Q000737:chemistry / Q000627:therapeutic use; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D000970:Antineoplastic Agents; D002457:Cell Extracts; D011134:Polysaccharides