The mouse Pol I terminator is more efficient than the hepatitis delta virus ribozyme in generating influenza-virus-like RNAs with precise 3' ends in a plasmid-only-based virus rescue system.
Reverse genetics systems for generating recombinant influenza viruses are based on two different mechanisms for obtaining the 3' end of the viral RNA: one uses the self-cleaving hepatitis delta virus ribozyme (HDVR), and the other uses the murine RNA polymerase I (Pol I) terminator. In this study, we employed EGFP and Renilla luciferase reporter constructs to compare the efficiency of both methods. Our results indicate that the murine Pol I terminator was more efficient than the HDVR, which will be helpful in choosing an influenza virus rescue system, as well as in establishing other RNA virus rescue systems.
Feng
Liqiang
L
Center for Vaccines and Biotherapeutics, Guangzhou Institute of Biomedicine and Health, China.
Li
Feng
F
Zheng
Xuehua
X
Pan
Weiqi
W
Zhou
Kai
K
Liu
Yichu
Y
He
Hongxuan
H
Chen
Ling
L
D020413:3' Untranslated Regions / Q000235:genetics; D000818:Animals; D002522:Chlorocebus aethiops; D012321:DNA-Directed RNA Polymerases / Q000235:genetics; D017930:Genes, Reporter; D016679:Genome, Viral; D049452:Green Fluorescent Proteins / Q000235:genetics; D003698:Hepatitis Delta Virus / Q000235:genetics*; D006801:Humans; D051379:Mice; D010957:Plasmids; D011401:Promoter Regions, Genetic; D012318:RNA Polymerase I / Q000235:genetics*; D016337:RNA, Catalytic / Q000235:genetics; D012367:RNA, Viral / Q000235:genetics; D015183:Restriction Mapping; D013728:Terminator Regions, Genetic; D014158:Transcription, Genetic; D014709:Vero Cells; D014779:Virus Replication
D020413:3' Untranslated Regions; D016337:RNA, Catalytic; D012367:RNA, Viral; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins; D012321:DNA-Directed RNA Polymerases; D012318:RNA Polymerase I