Bowmanella pacifica sp. nov., isolated from a pyrene-degrading consortium.
A taxonomic study was carried out on a strain, designated W3-3A(T), which was isolated from a pyrene-degrading consortium, enriched from sediment of the Pacific Ocean. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain W3-3A(T) belonged to the genus Bowmanella, with the highest sequence similarity (99.0 %) with Bowmanella denitrificans BD1(T), whereas sequence similarities with other species were less than 93 %. The nucleotide sequence similarity of both gyrB and rpoD genes of strain W3-3A(T) and B. denitrificans BD1(T) was 81.1 %. However, the protein sequence similarities of the gyrB and rpoD genes of strain W3-3A(T) and B. denitrificans BD1(T) were 96.1 % and 91.0 %, respectively. Phylogenetic trees based on these housekeeping genes showed that strain W3-3A(T) and B. denitrificans BD1(T) formed a distinct lineage in the Gammaproteobacteria. The DNA-DNA hybridization value between strain W3-3A(T) and B. denitrificans BD1(T) was 43 %. Strain W3-3A(T) could also be differentiated from B. denitrificans BD1(T) based on the repetitive extragenic palindromic DNA-PCR fingerprint. The G+C content of the chromosomal DNA of strain W3-3A(T) was 49 mol%. The combined genotypic and phenotypic data showed that strain W3-3A(T) represents a novel species of the genus Bowmanella, for which the name Bowmanella pacifica sp. nov. is proposed, with the type strain W3-3A(T) (=CGMCC 1.7086(T)=LMG 24568(T)=MCCC 1A01018(T)).
Lai
Qiliang
Q
Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography, State Oceanic Administration, Xiamen 361005, PR China.
Yuan
Jun
J
Wang
Baojiang
B
Sun
Fengqin
F
Qiao
Nan
N
Zheng
Tianling
T
Shao
Zongze
Z
D034241:Alteromonadaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology; D015373:Bacterial Typing Techniques; D001482:Base Composition; D001673:Biodegradation, Environmental; D027081:DNA Gyrase / Q000235:genetics; D004269:DNA, Bacterial / Q000032:analysis; D012321:DNA-Directed RNA Polymerases / Q000235:genetics; D020459:Genes, rRNA; D005838:Genotype; D019015:Geologic Sediments / Q000382:microbiology*; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010140:Pacific Ocean; D010641:Phenotype; D010802:Phylogeny; D011721:Pyrenes / Q000378:metabolism*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012808:Sigma Factor / Q000235:genetics; D013045:Species Specificity
D004269:DNA, Bacterial; D011721:Pyrenes; D012336:RNA, Ribosomal, 16S; D012808:Sigma Factor; C030984:pyrene; C081775:RNA polymerase sigma 70; D012321:DNA-Directed RNA Polymerases; D027081:DNA Gyrase