The GW182 protein family in animal cells: new insights into domains required for miRNA-mediated gene silencing.
GW182 family proteins interact directly with Argonaute proteins and are required for miRNA-mediated gene silencing in animal cells. The domains of the GW182 proteins have recently been studied to determine their role in silencing. These studies revealed that the middle and C-terminal regions function as an autonomous domain with a repressive function that is independent of both the interaction with Argonaute proteins and of P-body localization. Such findings reinforce the idea that GW182 proteins are key components of miRNA repressor complexes in metazoa.
Eulalio
Ana
A
Department of Biochemistry, Max Planck Institute for Developmental Biology, D-72076 Tübingen, Germany.
Tritschler
Felix
F
Izaurralde
Elisa
E
D000595:Amino Acid Sequence; D000818:Animals; D001324:Autoantigens / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D001665:Binding Sites / Q000235:genetics; D029742:Caenorhabditis elegans Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D017124:Conserved Sequence; D029721:Drosophila Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D039642:Eukaryotic Initiation Factors / Q000378:metabolism; D020868:Gene Silencing; D006801:Humans; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism*; D008957:Models, Genetic; D008958:Models, Molecular; D008969:Molecular Sequence Data; D017434:Protein Structure, Tertiary; D012323:RNA Processing, Post-Transcriptional; D016601:RNA-Binding Proteins; D017386:Sequence Homology, Amino Acid
D001324:Autoantigens; D029742:Caenorhabditis elegans Proteins; D029721:Drosophila Proteins; D039642:Eukaryotic Initiation Factors; C513405:Gw protein, Drosophila; D035683:MicroRNAs; D016601:RNA-Binding Proteins; C465663:TNRC6A protein, human