Neurite outgrowth is dependent on the association of c-Src and lipid rafts.
Regulation of neurite outgrowth is an important aspect not only for proper development of the nervous system but also for tissue regeneration after nerve injury and the treatment of neuropathological conditions. Here, we report that neurite outgrowth in cortical neuron and neuro 2A (N2A) cell was dependent on intact lipid rafts, as well as the enhanced localization of c-Src in the lipid rafts. Src inhibition or lipid rafts disruption could specifically block c-Src phosphorylation profile, pY416 Src increase and pY529 Src decrease, they also resulted in pY529 Src and c-terminal Src kinase (Csk) partition out of lipid rafts. Thus, we concluded that c-Src signal cascades within the lipid rafts is crucial for efficient neurite outgrowth.
Zhao
Hui
H
Department of Integrative Medicine and Neurobiology, National Key Lab of Medical Neurobiology, Institutes of Brain Research Sciences, Shanghai Medical College, Fudan University, 138 Yixueyuan Road, Box 291, 200032 Shanghai, People's Republic of China. mayzhao5375@yahoo.com
Cao
Xiaoding
X
Wu
Gencheng
G
Loh
Horace H
HH
Law
Ping-Yee
PY
D000818:Animals; D000081247:CSK Tyrosine-Protein Kinase; D021962:Membrane Microdomains / Q000502:physiology*; D051379:Mice; D016501:Neurites / Q000502:physiology*; D010766:Phosphorylation; D011505:Protein-Tyrosine Kinases / Q000378:metabolism*; D012038:Regeneration; D015398:Signal Transduction; D014443:Tyrosine / Q000378:metabolism; D019061:src-Family Kinases
D014443:Tyrosine; D011505:Protein-Tyrosine Kinases; D000081247:CSK Tyrosine-Protein Kinase; D019061:src-Family Kinases