GPCR microspot assays on solid substrates.
Multiplexing and miniaturization make microarrays an attractive tool for biomolecular interaction analysis. Adequate shelf life, mechanical stability through multiple assay steps, and amenability to a microplate format for screening are core requirements for the practical large-scale implementation of microarray technology. G protein-coupled receptor (GPCR) microarrays require the co-immobilization of the receptors and their associated lipid membranes. The vulnerability of solid-supported membranes to desorption and the unique surface requirements for GPCR function provide formidable challenges for the fabrication of GPCR microarrays. The chapter describes air-stable GPCR microarrays and their utility for selectivity profiling of GPCR drugs with high fidelity.
Fang
Ye
Y
Biochemical Technologies, Science and Technology Division, Corning Incorporated, Corning, NY, USA.
Lahiri
Joydeep
J
D001681:Biological Assay / Q000379:methods*; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D043562:Receptors, G-Protein-Coupled / Q000378:metabolism*
D043562:Receptors, G-Protein-Coupled