title: Podophyllin, but not the constituents quercetin or kaempferol, induced genotoxicity in vitro and in vivo through ROS production.
abstract: The genotoxic potential of podophyllin (PD) was investigated in this study. PD increased bacterial revertants and abnormal chromosomal structures in a concentration-dependent manner, both with and without metabolic activating enzymes, and increased the incidence of micronuclei in imprinted control region mouse reticulocytes. Results from three studied constituents of PD, such as podophyllotoxin, kampferol, and quercetin, suggested that the mutagenic effect of PD was not due to the presence of podophyllotoxin, kampferol, and quercetin and might be related to other components and the formation of reactive oxygen species. The detailed mutagenic mechanisms need further investigation, and the medicinal use of PD needs to be cautioned against.
authors.affiliation: College of Pharmacy, Taipei Medical University, Taipei, Taiwan.
authors.forename: Mei Chih
authors.initials: MC
authors.lastname: Lin
authors.forename: Hui Wen
authors.initials: HW
authors.lastname: Cheng
authors.forename: Yi Chen
authors.initials: YC
authors.lastname: Tsai
authors.forename: Po Lin
authors.initials: PL
authors.lastname: Liao
authors.forename: Jaw Jou
authors.initials: JJ
authors.lastname: Kang
authors.forename: Yu Wen
authors.initials: YW
authors.lastname: Cheng
mesh_terms: D000818:Animals; D002869:Chromosome Aberrations / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D044949:Kaempferols / Q000302:isolation & purification / Q000633:toxicity*; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D048629:Micronuclei, Chromosome-Defective / Q000187:drug effects; D009152:Mutagenicity Tests; D009153:Mutagens / Q000737:chemistry / Q000633:toxicity; D011033:Podophyllin / Q000008:administration & dosage / Q000737:chemistry / Q000633:toxicity*; D011034:Podophyllotoxin / Q000302:isolation & purification / Q000633:toxicity; D011794:Quercetin / Q000302:isolation & purification / Q000633:toxicity*; D051381:Rats; D017208:Rats, Wistar; D017382:Reactive Oxygen Species / Q000378:metabolism*; D012156:Reticulocytes / Q000187:drug effects / Q000378:metabolism
chemical_list: D044949:Kaempferols; D009153:Mutagens; D017382:Reactive Oxygen Species; C006552:kaempferol; D011033:Podophyllin; D011794:Quercetin; D011034:Podophyllotoxin