A new small-angle X-ray scattering set-up on the crystallography beamline I711 at MAX-lab.
A small-angle X-ray scattering (SAXS) set-up has recently been developed at beamline I711 at the MAX II storage ring in Lund (Sweden). An overview of the required modifications is presented here together with a number of application examples. The accessible q range in a SAXS experiment is 0.009-0.3 A(-1) for the standard set-up but depends on the sample-to-detector distance, detector offset, beamstop size and wavelength. The SAXS camera has been designed to have a low background and has three collinear slit sets for collimating the incident beam. The standard beam size is about 0.37 mm x 0.37 mm (full width at half-maximum) at the sample position, with a flux of 4 x 10(10) photons s(-1) and lambda = 1.1 A. The vacuum is of the order of 0.05 mbar in the unbroken beam path from the first slits until the exit window in front of the detector. A large sample chamber with a number of lead-throughs allows different sample environments to be mounted. This station is used for measurements on weakly scattering proteins in solutions and also for colloids, polymers and other nanoscale structures. A special application supported by the beamline is the effort to establish a micro-fluidic sample environment for structural analysis of samples that are only available in limited quantities. Overall, this work demonstrates how a cost-effective SAXS station can be constructed on a multipurpose beamline.
Knaapila
M
M
MAX-lab, Lund University, POB 118, SE-22100 Lund, Sweden.
Svensson
C
C
Barauskas
J
J
Zackrisson
M
M
Nielsen
S S
SS
Toft
K N
KN
Vestergaard
B
B
Arleth
L
L
Olsson
U
U
Pedersen
J S
JS
Cerenius
Y
Y
D018360:Crystallography, X-Ray / Q000295:instrumentation*; D007768:Lactalbumin / Q000737:chemistry; D040901:Proteomics; D053838:Scattering, Small Angle
D007768:Lactalbumin