title: Determination of aromatic amines in food products and composite food packaging bags by capillary electrophoresis coupled with transient isotachophoretic stacking.
abstract: A capillary electrophoretic method was explored to assay aromatic amines in food samples. With an inline-coupled transient isotachophoretic stacking approach, the method has yielded about 200-fold improvement of sensitivity in UV detection of three primary aromatic amines and melamine. By using K(+) as a leading ion and Tris(+) as a terminating ion, a plug of 10 cm (equivalent to 0.44 microL) sample solution was allowed to introduce into a 60 cm (50 cm effective) capillary for separation, giving limits of detection down to 2.0 x 10(-8)M. Baseline separation was achieved within 10 min, with relative standard deviation of 0.41-0.75% (intra-day) or 1.2-1.5% (inter-day) for migration time and 3.8-4.3% (intra-day) or 5.2-6.7% (inter-day) for peak area. The method was directly applicable to assaying the melamine in powder milk samples, with recovery in between 92.0% and 107.1%. The method could also be applied to the analysis of trace primary aromatic amines migrating from composite food packaging bags after combination of a 10-fold off-line concentration step, with limit of detection down to less than 1 microg/L. By this method, 4,4'-diaminophenylmethane and 2,4-diaminotoluene were thus found in three types of composite food packaging bags.
authors.affiliation: Laboratory of Analytical Chemistry for Life Science, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, China.
authors.forename: Xiaoyu
authors.initials: X
authors.lastname: Wang
authors.forename: Yi
authors.initials: Y
authors.lastname: Chen
mesh_terms: D000588:Amines / Q000032:analysis*; D000814:Aniline Compounds / Q000032:analysis; D000821:Animal Feed / Q000032:analysis; D000818:Animals; D000829:Animals, Domestic; D004586:Electrophoresis / Q000379:methods*; D019075:Electrophoresis, Capillary / Q000379:methods*; D005504:Food Analysis / Q000379:methods*; D018857:Food Packaging; D016014:Linear Models; D008892:Milk / Q000737:chemistry; D010655:Phenylenediamines / Q000032:analysis; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D014227:Triazines / Q000032:analysis; D001559:Benzhydryl Compounds
chemical_list: D000588:Amines; D000814:Aniline Compounds; D010655:Phenylenediamines; D014227:Triazines; C009505:4,4'-diaminodiphenylmethane; D001559:Benzhydryl Compounds; C010914:2,4-diaminotoluene; C011907:melamine