Toxic effects of hexaammine cobalt(III) chloride on liver and kidney in mice: Implication of oxidative stress.
Hexaammine cobalt (III) chloride is a trivalent complex cation of Co(III) and amine that has previously been shown to act as an inhibitor of insulin secretion, radiosensitizing agent, and an antiviral agent. We have recently reported the anticancer potential of the compound against diethylnitrosamine-induced carcinogenesis in mice. However, there is no report on the potential toxicity of the compound. The present study was conducted to evaluate the tissue distribution of the compound and its potential toxicity following acute administration of the compound through intraperitoneal route in Balb/c mice. Our results showed that cobalt accumulated maximally in kidney, followed by liver, spleen, blood, and lung in a decreasing order and in a dose-dependent manner. Evaluation of liver and kidney function tests revealed that the compound exerted a relatively higher toxicity in kidney, as compared to liver, as evidenced by the sharp enhancement in the serum levels of urea and creatinine in a dose-dependent manner. Examination of levels of lipid peroxidation and selected oxidative stress-related parameters in kidney, liver, and lung suggest that higher accumulation of cobalt in kidney may promote higher oxidative stress in the organ, as compared to liver and lung, which may eventually impair kidney function.
Naura
Amarjit S
AS
Department of Biochemistry, Panjab University, Chandigarh, India.
Sharma
Rajeshwar
R
D000818:Animals; D016588:Anticarcinogenic Agents / Q000493:pharmacokinetics / Q000633:toxicity*; D003035:Cobalt / Q000493:pharmacokinetics / Q000633:toxicity*; D003401:Creatine / Q000097:blood; D004305:Dose-Response Relationship, Drug; D007274:Injections, Intraperitoneal; D007668:Kidney / Q000187:drug effects / Q000378:metabolism; D007677:Kidney Function Tests; D015227:Lipid Peroxidation / Q000187:drug effects; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008111:Liver Function Tests; D008168:Lung / Q000187:drug effects / Q000378:metabolism; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D018384:Oxidative Stress / Q000187:drug effects* / Q000502:physiology; D013054:Spectrophotometry, Atomic; D014018:Tissue Distribution; D023382:Toxicity Tests, Acute; D014508:Urea / Q000097:blood
D016588:Anticarcinogenic Agents; C030862:cobalt ammonium complex; D003035:Cobalt; D014508:Urea; D003401:Creatine