title: Haemocyte lysosomal fragility facing an environmental reality: a toxicological perspective with atrazine and Lymnaea stagnalis (Gastropoda, Pulmonata) as a test case.
abstract: The present study investigates the potential use of haemocyte lysosomal fragility as a reliable indicator of toxic injury in snails subjected to herbicide atrazine. Two cytochemical assays were used. The first one was based on microscopic observations of neutral red dye, and the second one on a fluorescence spectroscopy approach using acridine orange, which had the advantage of being more reliable in assessing minor cell damage. Two sets of experiments were carried out in controlled laboratory conditions (temperature, photoperiod) and in outdoor microcosms, which produce more realistic ecological conditions. An unequivocal relationship was shown, linking atrazine exposure to Lymnaea stagnalis haemocytes lysosomal permeability. High temperatures also had a negative effect on the lysosomal membrane. In the microcosms, the response was affected by seasonal factors, and led to an integrated cell response. It was concluded that lysosomal sensitivity reflects environmental changes. Moreover, the technique using acridine orange was shown to be sufficiently sensitive and reliable to serve as a lysosomal marker in a controlled environment.
authors.affiliation: UMR 6553 Ecobio, Université Rennes I, CNRS, Campus de Beaulieu, avenue du Général Leclerc, 35042 Rennes, France. jacqueline.russo@univ-rennes1.fr
authors.forename: Russo
authors.initials: R
authors.lastname: Jacqueline
authors.forename: Madec
authors.initials: M
authors.lastname: Luc
authors.forename: Brehélin
authors.initials: B
authors.lastname: Michel
mesh_terms: D000165:Acridine Orange / Q000032:analysis; D000818:Animals; D001280:Atrazine / Q000633:toxicity*; D015415:Biomarkers; D002470:Cell Survival / Q000187:drug effects; D005456:Fluorescent Dyes / Q000032:analysis; D006434:Hemocytes / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D006540:Herbicides / Q000633:toxicity*; D007425:Intracellular Membranes / Q000187:drug effects; D008195:Lymnaea / Q000187:drug effects* / Q000378:metabolism; D008247:Lysosomes / Q000187:drug effects* / Q000378:metabolism; D010539:Permeability; D013696:Temperature
chemical_list: D015415:Biomarkers; D005456:Fluorescent Dyes; D006540:Herbicides; D000165:Acridine Orange; D001280:Atrazine