title: Regulation of nitrate transport in citrus rootstocks depending on nitrogen availability.
abstract: Previously, we reported that in Citrus plants, nitrate influx through the plasmalemma of roots cells follows a biphasic pattern, suggesting the existence of at least two different uptake systems, a high and low affinity transport system (HATS and LATS, respectively). Here, we describe a novel inducible high affinity transport system (iHATS). This new nitrate transport system has a high capacity to uptake nitrate in two different Citrus rootstocks (Cleopatra mandarin and Troyer citrange). The iHATS was saturable, showing higher affinity than constitutive high affinity transport system (cHATS) to the substrate NO(3) (-). The V(max) for this saturable component iHATS was higher than cHATS, reaching similar values in both rootstocks.Additionally, we studied the regulation of root NO(3) (-) uptake mediated by both HATS (iHATS and cHATS) and LATS. In both rootstocks, cHATS is constitutive and independent of N-status. Concerning the regulation of iHATS, this system is upregulated by NO(3) (-) and down-regulated by the N status and by NO(3) (-) itself when plants are exposed to it for a longer period of time. LATS in Cleopatra mandarin and Troyer citrange rootstocks is repressed by the N-status.The use of various metabolic uncouplers or inhibitors indicated that NO(3) (-) net uptake mediated by iHATS and LATS was an active transport system in both rootstocks.
authors.affiliation: Area de Fisiología Vegetal; Departamento de Ciencias Agrarias y del Medio Natural; ESTCE; Universitat Jaume I, Campus de Riu; Castellón, Spain.
authors.forename: Miguel
authors.initials: M
authors.lastname: Cerezo
authors.forename: Gemma
authors.initials: G
authors.lastname: Camañes
authors.forename: Víctor
authors.initials: V
authors.lastname: Flors
authors.forename: Eduardo
authors.initials: E
authors.lastname: Primo-Millo
authors.forename: Pilar
authors.initials: P
authors.lastname: García-Agustín