title: De novo synthesis of human dermis in vitro in the absence of a three-dimensional scaffold.
abstract: Neonatal human dermal fibroblasts cultured in vitro synthesize an organized and physically substantial three-dimensional extracellular matrix, without the addition of exogenous matrix components or synthetic scaffolds. De novo matrix synthesis proceeds in an orderly manner over a 21-d culture period and beyond. Analysis of the fibroblast phenotype, i.e., matrix synthesis by the fibroblasts, suggests that both serum and serum-free conditions are conducive to the production of a human tissue-engineered "dermal equivalent". We report that given the appropriate permissive environment, the fibroblasts establish and grow a tissue in vitro, which bears striking biochemical and physical resemblance to normal human dermis.
authors.affiliation: Research and Development, Organogenesis Inc, Canton, MA 02021, USA. t.pouyani@neu.edu
authors.forename: Tara
authors.initials: T
authors.lastname: Pouyani
authors.forename: Vincent
authors.initials: V
authors.lastname: Ronfard
authors.forename: Paul G
authors.initials: PG
authors.lastname: Scott
authors.forename: Carole M
authors.initials: CM
authors.lastname: Dodd
authors.forename: Aftab
authors.initials: A
authors.lastname: Ahmed
authors.forename: Richard L
authors.initials: RL
authors.lastname: Gallo
authors.forename: Nancy L
authors.initials: NL
authors.lastname: Parenteau
mesh_terms: D003094:Collagen / Q000378:metabolism* / Q000648:ultrastructure; D020405:Dermis / Q000502:physiology* / Q000648:ultrastructure; D004187:Disaccharides / Q000032:analysis; D005347:Fibroblasts / Q000502:physiology / Q000648:ultrastructure; D006801:Humans; D006820:Hyaluronic Acid / Q000096:biosynthesis; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D019063:Tenascin / Q000096:biosynthesis; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds
chemical_list: D004187:Disaccharides; D019063:Tenascin; D006820:Hyaluronic Acid; D003094:Collagen