[Effect of radiation on calcium-dependent pathway of nitroglycerin-evoked relaxation of rat aorta].
The effects of nitroglycerine (NG, 10(-7)-10(-4) M) on contractile force and intracellular calcium concentration ([Ca2+]i) in deendotelized thoracic aorta smooth muscles (SM) precontracted with high K+ in control and gamma-irradiated rats (6 Gy) were investigated. NG-induced activation of soluble guanylate cyclase (sGC) as dominating pathway of NG-induced SM relaxation without related decrease in [Ca2+]i level is demonstrated. Radiation was without effect on this sGC-dependent and [Ca2+]i -independent NG-induced relaxation of vascular smooth muscle but decreased the basal levels of[Ca2+]i by (28.4 +/- 11.9)% (6 Gy, 9th-day).
Zelens'kyĭ
S M
SM
Solovĭov
A I
AI
D000818:Animals; D001013:Aorta, Thoracic / Q000187:drug effects* / Q000378:metabolism / Q000528:radiation effects; D002118:Calcium / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D005720:Gamma Rays; D066298:In Vitro Techniques; D008297:Male; D009131:Muscle, Smooth, Vascular / Q000187:drug effects* / Q000378:metabolism / Q000528:radiation effects; D005996:Nitroglycerin / Q000494:pharmacology*; D051381:Rats; D011921:Rats, Inbred WKY; D014664:Vasodilation / Q000187:drug effects* / Q000528:radiation effects; D014665:Vasodilator Agents / Q000494:pharmacology*
D014665:Vasodilator Agents; D005996:Nitroglycerin; D002118:Calcium