Insulin-loaded poly(acrylic acid)-cysteine nanoparticles: stability studies towards digestive enzymes of the intestine.
This study evaluated thiolated poly(acrylic acid) nanoparticles as a valuable tool to protect insulin from degradation by serinproteases of the intestine. Nanaoparticles were characterized concerning particle size, zeta potential, and drug load. Furthermore, in vitro release studies were performed. Within in vitro degradation studies with trypsin, alpha-chymotrypsin, and elastase it could be demonstrated that the obtained nanoparticles are capable of protecting 44.47 +/- 0.89% of the initial insulin amount from tryptic degradation, 21.33 +/- 5.34% from chymotryptic degradation, and 45.01 +/- 1.40% from degradation by elastase compared to insulin solutions.
Perera
Glen
G
Institute of Pharmacy, Department of Pharmaceutical Technology, University of Innsbruck, Innsbruck, Austria.
Greindl
Melanie
M
Palmberger
Thomas F
TF
Bernkop-Schnürch
Andreas
A
D000179:Acrylates / Q000737:chemistry*; D000284:Administration, Oral; D002918:Chymotrypsin / Q000378:metabolism; D003545:Cysteine / Q000737:chemistry*; D004337:Drug Carriers / Q000008:administration & dosage / Q000378:metabolism; D004355:Drug Stability; D007004:Hypoglycemic Agents / Q000008:administration & dosage / Q000378:metabolism*; D007328:Insulin / Q000008:administration & dosage / Q000378:metabolism*; D007422:Intestines / Q000201:enzymology*; D053758:Nanoparticles / Q000737:chemistry*; D010196:Pancreatic Elastase / Q000378:metabolism; D012697:Serine Endopeptidases / Q000378:metabolism; D013678:Technology, Pharmaceutical; D014357:Trypsin / Q000378:metabolism
D000179:Acrylates; D004337:Drug Carriers; D007004:Hypoglycemic Agents; D007328:Insulin; D012697:Serine Endopeptidases; D002918:Chymotrypsin; C117608:alpha-chymotrypsin; D010196:Pancreatic Elastase; D014357:Trypsin; C036658:acrylic acid; D003545:Cysteine