Transcriptome analysis of hepatitis B virus-associated small hepatocellular carcinoma by serial analysis of gene expression.
Serial analysis of gene expression (SAGE) is a sequence-based tool that enables quantitative study of comprehensive gene expression profiles. In this study, we generated a SAGE library of hepatitis B virus (HBV)-associated small hepatocellular carcinoma (HCC) and compared it with libraries of normal liver and other organs in order to identify genes which are specifically expressed in HBV-associated small HCC. A total of 18,107 SAGE tags were obtained from the HCC SAGE library. Among the 7,405 unique tags, 240 were significantly overexpressed in HCC compared to normal liver. Seventeen genes were unequivocally matched to SAGE tags which were up-regulated 15-fold or higher in HCC compared to normal liver: four genes (fatty acid desaturase 2, alpha-L-2-fucosidase, testis-specific protein Y-encoded-like 2 and Gon-4-like) were significantly overexpressed in the HCC SAGE library compared to any other SAGE libraries of human normal tissues. The significance of these genes with respect to carcinogenesis and early diagnosis of HCC needs to be elucidated in further studies.
Kim
Jin-Wook
JW
Department of Medicine, Seoul National University Bundang Hospital, Bundang-gu, Seongnam 463-802, Korea. kimjw@snubh.org
Lee
Sang Hyub
SH
Park
Young Soo
YS
Jeong
Sook-Hyang
SH
Kim
Nayoung
N
Lee
Dong Ho
DH
Lee
Hyo-Suk
HS
D000328:Adult; D000368:Aged; D044466:Asian People / Q000235:genetics; D014408:Biomarkers, Tumor / Q000235:genetics*; D006528:Carcinoma, Hepatocellular / Q000208:ethnology / Q000235:genetics* / Q000821:virology; D005260:Female; D020869:Gene Expression Profiling / Q000379:methods*; D015972:Gene Expression Regulation, Neoplastic; D015723:Gene Library; D006509:Hepatitis B / Q000150:complications* / Q000208:ethnology; D006801:Humans; D007723:Korea; D008113:Liver Neoplasms / Q000208:ethnology / Q000235:genetics* / Q000821:virology; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D015203:Reproducibility of Results; D017422:Sequence Analysis, DNA
D014408:Biomarkers, Tumor