title: [Construction of retroviral vector with human brain-derived neurotrophic factor gene expression and in the fibroblasts expression].
abstract: OBJECTIVE
  To construct human brain-derived neurotrophic factor retroviral vector-pLXSN (hBDNF-pLXSN), and to evaluate the bioactivity of hBDNF.
  
  
  METHODS
  The genome mRNA was extracted from embryonic brain tissue of a 5-month-old infant, the hBDNF gene sequence was obtained with RT-PCR technology, and hBDNF-pLXSN constructed in vitro was used to infect the fibroblasts (NIH/3T3). The expression of hBDNF was identfied by the immunohistochemistry method, and the NIH/3T3 and BDNF biological activities were determined by culture of the PC12 cells and dorsal root ganglia.
  
  
  RESULTS
  The hBDNF-pLXSN was constructed successfully by sequencing analyses. The infected NIH/3T3 showed positive expression of hBDNF. The infected NIH/3T3 could product hBDNF. Bioactivity of the products could support the PC12 cell survival and neurite growth in the primary cultures of dorsal root ganglia neurons of mice.
  
  
  CONCLUSIONS
  hBDNF-pLXSN virus has the ability to infect NIH/3T3 and make it expressed and secreted hBDNF with the biological activity. It can be used to treat facial paralysis as a gene therapy.
authors.affiliation: Stomatological Center, General Hospital of Chinese People's Armed Police Forces, Beijing, 100039, P.R. China.
authors.forename: Cheng
authors.initials: C
authors.lastname: Yang
authors.forename: Rongzeng
authors.initials: R
authors.lastname: Yan
authors.forename: Hongyan
authors.initials: H
authors.lastname: Guo
authors.forename: Xiaoming
authors.initials: X
authors.lastname: Gu
mesh_terms: D000818:Animals; D019208:Brain-Derived Neurotrophic Factor / Q000235:genetics*; D002478:Cells, Cultured; D005347:Fibroblasts; D005822:Genetic Vectors; D006801:Humans; D051379:Mice; D041681:NIH 3T3 Cells; D016716:PC12 Cells; D051381:Rats; D017207:Rats, Sprague-Dawley; D012190:Retroviridae / Q000235:genetics; D014162:Transfection
chemical_list: D019208:Brain-Derived Neurotrophic Factor