Characterization of cytotoxic necrotizing factor 1-producing Escherichia coli strains from faeces of healthy macaques.
Twenty-five (27 %) of 92 clinically normal macaques were found to have beta-haemolytic Escherichia coli isolated from their faeces. Five of six isolates chosen for further characterization had multiple antibiotic resistance and were PCR-positive for cytotoxic necrotizing factor 1 (cnf1) with a demonstrated cytopathic effect in vitro. By repetitive element sequence-based PCR genotyping, genetic similarity was established for selected isolates. We believe this to be the first report of E. coli strains producing CNF1 in non-human primates.
Martin
Heather R
HR
Division of Comparative Medicine, Massachusetts Institute of Technology, Cambridge, MA, USA.
Taylor
Nancy S
NS
Division of Comparative Medicine, Massachusetts Institute of Technology, Cambridge, MA, USA.
Buckley
Ellen M
EM
Division of Comparative Medicine, Massachusetts Institute of Technology, Cambridge, MA, USA.
Marini
Robert P
RP
Division of Comparative Medicine, Massachusetts Institute of Technology, Cambridge, MA, USA.
Patterson
Mary M
MM
Division of Comparative Medicine, Massachusetts Institute of Technology, Cambridge, MA, USA.
Fox
James G
JG
Division of Comparative Medicine, Massachusetts Institute of Technology, Cambridge, MA, USA.
D000818:Animals; D001427:Bacterial Toxins / Q000096:biosynthesis* / Q000235:genetics; D016172:DNA Fingerprinting; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification; D004926:Escherichia coli / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000472:pathogenicity; D029968:Escherichia coli Proteins / Q000096:biosynthesis* / Q000235:genetics; D005243:Feces / Q000382:microbiology; D005798:Genes, Bacterial; D006367:HeLa Cells; D006801:Humans; D008252:Macaca fascicularis / Q000382:microbiology*; D008253:Macaca mulatta / Q000382:microbiology*; D012703:Serotyping; D014774:Virulence / Q000235:genetics
D001427:Bacterial Toxins; D004269:DNA, Bacterial; D029968:Escherichia coli Proteins; C060296:cytotoxic necrotizing factor type 1