Improved adherence and spreading of Saos-2 cells on polypropylene surfaces achieved by surface texturing and carbon nitride coating.
The adhesion and contact guidance of human primary osteogenic sarcoma cells (Saos-2) were characterized on smooth, microstructured (MST) and micro- and nano-structured (MNST) polypropylene (PP) and on the same samples with a silicon-doped carbon nitride (C(3)N(4)-Si) coating. Injection molding was used to pattern the PP surfaces and the coating was obtained by using ultra-short pulsed laser deposition (USPLD). Surfaces were characterized using atomic force microscopy and surface energy components were calculated according to the Owens-Wendt model. The results showed C(3)N(4)-Si coated surfaces to be significantly more hydrophilic than uncoated ones. In addition, there were 86% more cells in the smooth C(3)N(4)-Si coated PP compared to smooth uncoated PP and 551%/476% more cells with MST/MNST C(3)N(4)-Si coated PP than could be obtained with MST/MNST uncoated PP. Thus the adhesion, spreading and contact guidance of osteoblast-like cells was effectively improved by combining surface texturing and deposition of osteocompatible C(3)N(4)-Si coating.
Myllymaa
Katja
K
Department of Physics, University of Kuopio, PO Box 1627, 70211 Kuopio, Finland. katja.myllymaa@uku.fi
Myllymaa
Sami
S
Korhonen
Hannu
H
Lammi
Mikko J
MJ
Saarenpää
Hanna
H
Suvanto
Mika
M
Pakkanen
Tapani A
TA
Tiitu
Virpi
V
Lappalainen
Reijo
R
D001672:Biocompatible Materials / Q000737:chemistry; D002244:Carbon / Q000737:chemistry; D002448:Cell Adhesion; D045744:Cell Line, Tumor; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D007834:Lasers; D018625:Microscopy, Atomic Force / Q000379:methods; D008855:Microscopy, Electron, Scanning / Q000379:methods; D053758:Nanoparticles / Q000737:chemistry; D036103:Nanotechnology / Q000379:methods; D009570:Nitriles / Q000737:chemistry*; D009584:Nitrogen / Q000737:chemistry; D010006:Osteoblasts / Q000166:cytology; D011126:Polypropylenes / Q000737:chemistry*; D012825:Silicon / Q000737:chemistry; D013499:Surface Properties; D013778:Tetrazolium Salts / Q000494:pharmacology; D013844:Thiazoles / Q000494:pharmacology; D013997:Time Factors
D001672:Biocompatible Materials; D009570:Nitriles; D011126:Polypropylenes; D013778:Tetrazolium Salts; D013844:Thiazoles; C011206:cyanogen; D002244:Carbon; C022616:thiazolyl blue; D009584:Nitrogen; D012825:Silicon