Synthesis, characterization, DNA binding and cleavage studies of chiral Ru(II) salen complexes.
Interaction of chiral Ru(II) salen complexes (S)-1 and (R)-1 with Calf Thymus DNA (CT-DNA) was studied by absorption spectroscopy, competitive binding study, viscosity measurements, CD measurements, thermal denaturation study and cleavage studies by agarose gel electrophoresis. The DNA binding affinity of (S)-1 (6.25 x 10(3)M(-1)) was found to be greater than (R)-1 (3.0 x 10(3)M(-1)). The antimicrobial studies of these complexes on five different gram (+)/(-) bacteria and three different fungal organisms showed selective inhibition of the growth of gram (+) bacteria and were not affective against gram (-) and fungal organisms. Further, the (S)-1 enantiomer inhibited the growth of organisms to a greater extent as compared to (R)-1 enantiomer.
Khan
Noor-ul H
NU
Discipline of Inorganic Materials and Catalysis, Central Salt and Marine Chemicals Research Institute, Council of Scientific & Industrial Research, GB Marg, Bhavnagar 364002, Gujarat, India. khan2593@yahoo.co.in
Pandya
Nirali
N
Kureshy
Rukhsana I
RI
Abdi
Sayed H R
SH
Agrawal
Santosh
S
Bajaj
Hari C
HC
Pandya
Jagruti
J
Gupte
Akashya
A
D000890:Anti-Infective Agents / Q000138:chemical synthesis / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D001547:Benzaldehydes / Q000138:chemical synthesis* / Q000737:chemistry* / Q000378:metabolism* / Q000494:pharmacology; D001667:Binding, Competitive; D004247:DNA / Q000378:metabolism*; D053904:DNA Breaks, Single-Stranded / Q000187:drug effects; D005029:Ethylenediamines / Q000737:chemistry*; D046911:Macromolecular Substances / Q000138:chemical synthesis / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D008826:Microbial Sensitivity Tests; D008954:Models, Biological; D009691:Nucleic Acid Denaturation; D012428:Ruthenium / Q000737:chemistry*; D013057:Spectrum Analysis; D013237:Stereoisomerism; D013696:Temperature; D014783:Viscosity
D000890:Anti-Infective Agents; D001547:Benzaldehydes; D005029:Ethylenediamines; D046911:Macromolecular Substances; D012428:Ruthenium; D004247:DNA; C427203:calf thymus DNA; C011452:disalicylaldehyde ethylenediamine