Preventive effect of Tinospora cordifolia against high-fructose diet-induced insulin resistance and oxidative stress in male Wistar rats.
High intake of dietary fructose exerts a number of adverse metabolic effects. The aim of the present study was to investigate whether aqueous extract of Tinospora cordifolia stem (TCAE) alleviates high-fructose diet-induced insulin resistance and oxidative stress in rats. High-fructose diet (66% of fructose) and TCAE (400 mg/kg/day) were given simultaneously for a period of 60 days. Fructose fed rats showed hyperglycemia, hyperinsulinemia, hypertriglyceridemia, impaired glucose tolerance and impaired insulin sensitivity (P<0.05). TCAE treatment prevented the rise in glucose levels by 21.3%, insulin by 51.5%, triglycerides by 54.12% and glucose-insulin index by 59.8% of the fructose fed rats. Regarding liver antioxidant status, fructose fed rats showed higher values of lipid peroxidation (91.3%), protein carbonyl groups (44%) and lowered GSH levels (42.1%) and, lowered activities of enzymatic antioxidants, while TCAE treatment prevented all these observed abnormalities. In conclusion, our data indicate the preventive role of T. cordifolia against fructose-induced insulin resistance and oxidative stress; hence this plant could be used as an adjuvant therapy for the prevention and/or management of chronic diseases characterized by hyperinsulinemia, hypertriglyceridemia, insulin resistance and aggravated antioxidant status.
Reddy
Singareddy Sreenivasa
SS
Department of Biochemistry, Sri Krishnadevaraya University, Anantapur, Andhra Pradesh, India.
Ramatholisamma
Pasurla
P
Karuna
Rasineni
R
Saralakumari
Desireddy
D
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D004195:Disease Models, Animal; D004336:Drug Antagonism; D005632:Fructose / Q000009:adverse effects* / Q000378:metabolism; D005951:Glucose Tolerance Test; D005978:Glutathione / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects / Q000502:physiology; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008297:Male; D024821:Metabolic Syndrome / Q000209:etiology / Q000378:metabolism / Q000517:prevention & control*; D018384:Oxidative Stress / Q000187:drug effects* / Q000502:physiology; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D050050:Protein Carbonylation / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar; D031610:Tinospora / Q000737:chemistry*
D000975:Antioxidants; D010936:Plant Extracts; D005632:Fructose; D005978:Glutathione