Role of poly-L-lysine-coated plates and fetal calf serum concentration in sheep chondroprogenitor cell culturing.
Conventional methods for differentiation of chondroprogenitor cells on plastic plates face several problems that hinder the application of this method for the treatment of chondrogenic injury. This work focused on the effect of poly-L-lysine (PLL)-coated plastic surfaces and fetal calf serum concentration on the chondroprogenitor cells. In the present study, cartilage was isolated from the articular cartilages of sheep and the cells were seeded on PLL-coated plates in various serum concentrations. Histochemical analysis was used to determine chondrogenic differentiation of the cells. According to our results, the cells formed three-dimensional masses and chondrogenic cells. In the present investigation, the best culture conditions for maximum proliferation of isolated cells were examined. Taken together, the results indicated that PLL may have some effect on the adhesive properties of chondroprogenitor cells and could be used for cartilage engineering.
Atashi
Amir
A
Hematology Department, Faculty of Medical Science, Tarbiat Modares University, Tehran, Iran.
Nadri
Samad
S
Hafizi
Maryam
M
Soleimani
Masoud
M
D000818:Animals; D002356:Cartilage / Q000166:cytology*; D002417:Cattle; D018929:Cell Culture Techniques; D049109:Cell Proliferation; D003470:Culture Media; D007150:Immunohistochemistry; D011107:Polylysine; D044967:Serum; D012756:Sheep; D013234:Stem Cells
D003470:Culture Media; D011107:Polylysine