Simultaneous evaluation of chemiluminescence and bioluminescence in a phagocytic system.
The spectra of luminol-dependent chemiluminescence of leukocytes, forming as a result of their oxygen-dependent bactericidal systems activation, and bacterial bioluminescence of Escherichia coli recombinant strain with cloned lux operon, used as the object of phagocytosis, are not identical. Mutual overlapping of these spectra reaches 87%, including overlapping of the photoemission maximums. However these spectra can be evaluated separately in the short-wave "arm" of the chemiluminescence spectrum (<420 nm) and the long-wave "arm" of the bioluminescence spectrum (>560). The kinetics of luminol-dependent chemiluminescence of phagocytes and of bacterial bioluminescence in their mixtures is characterized by mutually dependent phase-wise changes in the intensity of the analyzed parameters.
Deryabin
D G
DG
Orenburg State University, Russia. dgderyabin@yandex.ru
Karimov
I F
IF
D004926:Escherichia coli / Q000378:metabolism; D006801:Humans; D049449:Luminescence; D008163:Luminescent Measurements / Q000295:instrumentation* / Q000379:methods*; D008165:Luminol / Q000737:chemistry; D010586:Phagocytes / Q000378:metabolism*; D010587:Phagocytosis / Q000502:physiology
D008165:Luminol