title: Efficacy of a genetically-modified Salmonella typhimurium in an orthotopic human pancreatic cancer in nude mice.
abstract: We report here a tumor-targeting strategy for pancreatic cancer using a modified auxotrophic strain of Salmonella typhimurium. The genetically-modified strain of S. typhimurium requires the amino acids arginine and leucine. These mutations preclude growth in normal tissue but do not reduce bacterial virulence in tumor cells. The tumor-targeting strain of S. typhimurium, termed A1-R and expressing green fluorescent protein (GFP), was administered to an orthotopic human pancreatic tumor expressing red fluorescent protein (RFP) in nude mice. After 7 days of treatment, the pancreatic cancer had regressed without the need of chemotherapy or any other treatment. This new strategy demonstrates the clinical potential of bacterial targeting for pancreatic cancer.
authors.affiliation: AntiCancer, Inc., San Diego, CA 92111, U.S.A.
authors.forename: Chisa
authors.initials: C
authors.lastname: Nagakura
authors.forename: Katsuhiro
authors.initials: K
authors.lastname: Hayashi
authors.forename: Ming
authors.initials: M
authors.lastname: Zhao
authors.forename: Kensuke
authors.initials: K
authors.lastname: Yamauchi
authors.forename: Norio
authors.initials: N
authors.lastname: Yamamoto
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Tsuchiya
authors.forename: Katsuro
authors.initials: K
authors.lastname: Tomita
authors.forename: Michael
authors.initials: M
authors.lastname: Bouvet
authors.forename: Robert M
authors.initials: RM
authors.lastname: Hoffman
mesh_terms: D000818:Animals; D001120:Arginine / Q000378:metabolism; D045744:Cell Line, Tumor; D049452:Green Fluorescent Proteins / Q000235:genetics; D006801:Humans; D007930:Leucine / Q000378:metabolism; D051379:Mice; D008819:Mice, Nude; D009154:Mutation / Q000235:genetics; D030781:Organisms, Genetically Modified; D010190:Pancreatic Neoplasms / Q000382:microbiology* / Q000628:therapy*; D012486:Salmonella typhimurium / Q000502:physiology*; D014162:Transfection; D023041:Xenograft Model Antitumor Assays
chemical_list: D049452:Green Fluorescent Proteins; D001120:Arginine; D007930:Leucine