Renal capsule-parathymic lymph node complex: a new in vivo metastatic model in rats.
BACKGROUND
The ultimate cause of cancer death is, in most cases, the appearance of metastases. The aim of the present study was to contribute to animal experimental investigations of metastatic tumor development.


METHODS
Rat hepatocarcinoma (He/De), mesoblastic nephroma (Ne/De) cells, and in other cases tumor-bearing lymph nodes were transplanted under the renal capsule of F344 rats. Metastatic potential of tumor cells was examined by whole body autoradiography and phosphor image analysis. The organ distribution of cells was also investigated.


RESULTS
Transplanted tumor cells resulted in metastases in the parathymic lymph nodes. Implanted India ink also demonstrated connection between the lymphatic vessels of the renal capsule and the parathymic lymph nodes. The metastatic potential was independent of the primary tumor growth rate.


CONCLUSIONS
The renal capsule-parathymic lymph node complex seems to be suitable for the isolated in vivo examination of metastatic development and for the detailed analysis of secondary tumors.
Trencsenyi
Gyorgy
G
Department of Microbial Biotechnology and Cell Biology, Faculty of Medicine, University of Debrecen, Debrecen, Hungary.
Kertai
Pal
P
Bako
Fruzsina
F
Hunyadi
Janos
J
Marian
Terez
T
Hargitai
Zoltan
Z
Pocsi
Istvan
I
Muranyi
Edit
E
Hornyak
Laszlo
L
Banfalvi
Gaspar
G
D000818:Animals; D002292:Carcinoma, Renal Cell / Q000473:pathology / Q000556:secondary*; D004195:Disease Models, Animal; D005260:Female; D007680:Kidney Neoplasms / Q000473:pathology / Q000556:secondary*; D008113:Liver Neoplasms / Q000473:pathology*; D008198:Lymph Nodes / Q000473:pathology*; D008207:Lymphatic Metastasis; D008297:Male; D051381:Rats; D011916:Rats, Inbred F344; D013370:Subrenal Capsule Assay; D013950:Thymus Gland / Q000473:pathology*; D014407:Tumor Cells, Cultured; D009396:Wilms Tumor / Q000473:pathology / Q000556:secondary*