Molecular characterization of hypoxia inducible factor-1 (HIF-1) from the white shrimp Litopenaeus vannamei and tissue-specific expression under hypoxia.
Hypoxia inducible factor 1 (HIF-1) is a key transcription factor that regulates a variety of molecular responses to hypoxia. Some marine crustaceans experience changes of oxygen tension in their aquatic environment, but knowledge about the function and expression of HIF-1 is very limited. HIF-1 is a heterodimer composed by alpha and beta subunits. We report the complete cDNA sequences of HIF-1alpha and HIF-1beta from the white shrimp Litopenaeus vannamei. HIF-1alpha (LvHIF-1alpha) is 3672bp and codes for 1050 amino acids, while HIF-1beta is 2135bp (LvHIF-1beta) and 608 amino acids. Both, the alpha and beta subunits have the helix-loop-helix (bHLH) and PAS domains. HIF-1alpha also has the oxygen dependent degradation (ODD) and the C-terminal transactivation domain (C-TAD), important for regulation in normoxia. Phylogenetic analyses of the proteins indicate separation of invertebrates from vertebrates. Large differences of HIF-1alpha and HIF-1beta transcripts abundance were detected in gills, hepatopancreas and muscle under normoxia (6mg/L dissolved oxygen, DO) and hypoxia (2.5 and 1.5mg/L DO). HIF-1alpha was more abundant in gills and HIF-1beta in hepatopancreas. Large changes in response to hypoxia were detected for HIF-1alpha in gills, while HIF-1beta remained fairly constant. Glucose and lactate in hemolymph increased rapidly in hypoxia in all cases and up to 4.7 and 5.0-fold, respectively, in response to 1.5mg/L DO for 1h.
Soñanez-Organis
José G
JG
Centro de Investigación en Alimentación y Desarrollo (CIAD), Sonora, Mexico.
Peregrino-Uriarte
Alma B
AB
Gómez-Jiménez
Silvia
S
López-Zavala
Alonso
A
Forman
Henry Jay
HJ
Yepiz-Plascencia
Gloria
G
D000595:Amino Acid Sequence; D000818:Animals; D051784:Aryl Hydrocarbon Receptor Nuclear Translocator / Q000235:genetics; D001483:Base Sequence; D018076:DNA, Complementary / Q000737:chemistry; D005880:Gills / Q000378:metabolism; D043143:Hepatopancreas / Q000378:metabolism; D000860:Hypoxia / Q000378:metabolism*; D051793:Hypoxia-Inducible Factor 1 / Q000235:genetics / Q000378:metabolism*; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000235:genetics; D008969:Molecular Sequence Data; D033561:Penaeidae / Q000235:genetics / Q000378:metabolism; D010802:Phylogeny
D018076:DNA, Complementary; D051793:Hypoxia-Inducible Factor 1; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D051784:Aryl Hydrocarbon Receptor Nuclear Translocator