Glycan-binding profile and cell adhesion activity of American bullfrog (Rana catesbeiana) oocyte galectin-1.
The glycan-binding profile of a beta-galactoside-binding 15 kDa lectin (Galectin-1) purified from the oocytes of the American bullfrog, Rana catesbeiana, was studied using 61 pyridyl-aminated oligosaccharides by frontal affinity chromatography. Human blood type-A-hexasaccharide (GalNAcalpha1-3(Fucalpha1-2)Galbeta;1-4GlcNAcbeta1-4Galbeta1-4Glc) was found to exhibit the strongest ligand binding to the galectin while Forssman antigen (GalNAcalpha1-3GalNAcbeta1-3Galalpha1-4Galbeta1-4Glc) and type-A-tetrasaccharide (GalNAcalpha1-3(Fucalpha1-2)Galbeta1-4GlcNAcbeta1-4Glc) were also extensively recognized. The kinetics of affinity of galectin-1 to type-A oligosaccharide was analysed by surface plasmon resonance using neoglycoprotein with type-A oligosaccharides. R. catesbeiana oocyte galectin adhered to human rhabdomyosarcoma cells dose dependently and the activity was specifically cancelled by the neoglycoprotein. It was concluded that galectin-1 from R. catesbeiana oocytes possesses different and rare glycan-binding properties from typical members in galectin family.
Kawsar
Sarkar M A
SM
Department of Environmental Biosciences, Research Units of Marine Biochemistry and Developmental Biology, International Graduate School of Arts and Sciences, Yokohama City University, Yokohama, Japan.
Matsumoto
Ryo
R
Fujii
Yuki
Y
Yasumitsu
Hidetaro
H
Uchiyama
Hideho
H
Hosono
Masahiro
M
Nitta
Kazuo
K
Hamako
Jiharu
J
Matsui
Taei
T
Kojima
Noriaki
N
Ozeki
Yasuhiro
Y
D000017:ABO Blood-Group System / Q000378:metabolism; D000818:Animals; D002448:Cell Adhesion; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D005260:Female; D005577:Forssman Antigen / Q000378:metabolism; D037483:Galectin 1 / Q000378:metabolism*; D006801:Humans; D007700:Kinetics; D009865:Oocytes / Q000737:chemistry*; D011134:Polysaccharides / Q000378:metabolism*; D011485:Protein Binding; D011892:Rana catesbeiana; D012208:Rhabdomyosarcoma / Q000378:metabolism; D020349:Surface Plasmon Resonance
D000017:ABO Blood-Group System; D037483:Galectin 1; D011134:Polysaccharides; D005577:Forssman Antigen