Determination of ligustilide in rat brain after nasal administration of essential oil from Rhizoma Chuanxiong.
A simple and sensitive HPLC-UV method was developed to determine ligustilide in rat brain samples after nasal administration. The lower limit of quantification (LLOQ) was 245 ng/ml for ligustilide. The calibration curve was linear over a concentration range of 245-4900 ng/ml. Brain samples were obtained at regular time intervals after nasal administration of ligustilide (45 mg/kg). Ligustilide could be detected in rat brain only after 5 min of nasal administration; which showed that ligustilide may have a rapid onset of action. This result illustrates that intranasal administration of ligustilide may act as a promising alternative to conventional routes of administration.
Guo
Jianming
J
Jiangsu Key Laboratory for TCM Formulae Research, Nanjing University of Chinese Medicine, Nanjing 210046, PR China.
Duan
Jin-ao
JA
Shang
Er-xin
EX
Tang
Yuping
Y
Qian
Dawei
D
D015107:4-Butyrolactone / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000032:analysis / Q000493:pharmacokinetics; D000280:Administration, Inhalation; D000818:Animals; D001921:Brain / Q000378:metabolism*; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D005260:Female; D030004:Ligusticum / Q000737:chemistry*; D008297:Male; D009822:Oils, Volatile / Q000008:administration & dosage; D051381:Rats; D017207:Rats, Sprague-Dawley; D027343:Rhizome
D004365:Drugs, Chinese Herbal; D009822:Oils, Volatile; C027820:ligustilide; D015107:4-Butyrolactone; C101574:chuangxiong extract