title: Receptor binding activities of Chlorella on cysteinyl leukotriene CysLT, glutamate AMPA, ion channels, purinergic P 2Y, tachykinin NK2 receptors and adenosine transporter.
abstract: A Chlorella powder was tested in a total of 129 in vitro receptor binding assay systems. The results showed a potent inhibition of this powder on cysteinyl leukotriene CysLT2, and glutamate AMPA in a dose-concentration manner with IC(50) mean +/- SEM values of 20 +/- 4.5 microg/mL and 44 +/- 14 microg/mL, respectively. Other moderate and weak activities reflected in competitive binding experiments were seen versus adenosine transporter; calcium channel L-type, benzothiazepine; gabapentin; kainate, NMDA-glycine; inositol trisphosphate IP(3); cysteinyl CysLT(1), LTB(4); purinergic P(2Y); tachykinin NK(2); serotonin 5-HT(2B) and prostanoid, thromboxane A(2). Together, the results suggest that the various inhibitory effects of Chlorella powder in these receptor binding assays could reflect its actions in modulating Ca(2+)-dependent signal related targets and might be relevant to the mechanisms of its biological effects. These results reveal important potential biochemical activities that might be exploited for the prevention or treatment of several pathologies. From these results, the possible therapeutic usage of the product is discussed.
authors.affiliation: MDS Pharma Services Taiwan Ltd, 158 Li-Teh Road, Peitou, Taipei, 112, Taiwan, ROC. fong-chi.cheng@mdsinc.com
authors.forename: Fong-Chi
authors.initials: FC
authors.lastname: Cheng
authors.forename: Jin-Jye
authors.initials: JJ
authors.lastname: Feng
authors.forename: Kuo-Hsin
authors.initials: KH
authors.lastname: Chen
authors.forename: Hideyo
authors.initials: H
authors.lastname: Imanishi
authors.forename: Masaki
authors.initials: M
authors.lastname: Fujishima
authors.forename: Hideo
authors.initials: H
authors.lastname: Takekoshi
authors.forename: Yo
authors.initials: Y
authors.lastname: Naoki
authors.forename: Minoru
authors.initials: M
authors.lastname: Shimoda
mesh_terms: D000818:Animals; D016466:CHO Cells; D045744:Cell Line, Tumor; D002708:Chlorella / Q000737:chemistry*; D006224:Cricetinae; D003412:Cricetulus; D004305:Dose-Response Relationship, Drug; D006168:Guinea Pigs; D006801:Humans; D020128:Inhibitory Concentration 50; D007473:Ion Channels / Q000378:metabolism*; D008297:Male; D033703:Nucleoside Transport Proteins / Q000378:metabolism*; D011485:Protein Binding; D051381:Rats; D017208:Rats, Wistar; D018077:Receptors, Leukotriene / Q000378:metabolism*; D017981:Receptors, Neurotransmitter / Q000378:metabolism*
chemical_list: D007473:Ion Channels; D033703:Nucleoside Transport Proteins; D018077:Receptors, Leukotriene; D017981:Receptors, Neurotransmitter; C444719:adenosine transporter