title: Analytical sensitivity of rapid influenza antigen detection tests for swine-origin influenza virus (H1N1).
abstract: BACKGROUND
  A novel swine origin influenza virus (S-OIV) (H1N1) is spreading worldwide and threatens to become pandemic.
  
  
  OBJECTIVE
  Determine analytical sensitivity of selected commercially available rapid influenza antigen detection tests in detecting S-OIV H1N1.
  
  
  METHODS
  Serial dilutions of two S-OIV isolates, one seasonal influenza A (H1N1) isolate and a nasopharyngeal aspirate from a patient with S-OIV disease were tested in five commercially available influenza antigen detection tests and by virus isolation in cell culture. Viral M gene copy number was determined by quantitative PCR methods.
  
  
  RESULTS
  The analytical sensitivity of the five influenza antigen detection tests for S-OIV (tissue culture infectious dose 50 (TCID(50)) log(10)3.3-4.7 was comparable with that of seasonal influenza (TCID(50) log(10)4.0-4.5).
  
  
  CONCLUSIONS
  The analytical sensitivity of the selected influenza A antigen detection tests for detection of S-IOV was comparable with that of seasonal influenza H1N1.
authors.affiliation: Department of Microbiology, The University of Hong Kong, Hong Kong Special Administrative Region, China.
authors.forename: K H
authors.initials: KH
authors.lastname: Chan
authors.forename: S T
authors.initials: ST
authors.lastname: Lai
authors.forename: L L M
authors.initials: LL
authors.lastname: Poon
authors.forename: Y
authors.initials: Y
authors.lastname: Guan
authors.forename: K Y
authors.initials: KY
authors.lastname: Yuen
authors.forename: J S M
authors.initials: JS
authors.lastname: Peiris
mesh_terms: D000818:Animals; D000956:Antigens, Viral / Q000032:analysis* / Q000276:immunology; D006801:Humans; D007118:Immunoassay / Q000379:methods; D053118:Influenza A Virus, H1N1 Subtype / Q000276:immunology / Q000302:isolation & purification*; D007251:Influenza, Human / Q000175:diagnosis* / Q000821:virology*; D011933:Reagent Kits, Diagnostic; D016865:Reassortant Viruses / Q000276:immunology / Q000302:isolation & purification*; D012680:Sensitivity and Specificity; D013552:Swine
chemical_list: D000956:Antigens, Viral; D011933:Reagent Kits, Diagnostic