Thermodynamic origin of cooperativity in actomyosin interactions: the coupling of short-range interactions with actin bending stiffness in an Ising-like model.
We present Monte Carlo simulations for a molecular motor system found in virtually all eukaryotic cells, the acto-myosin motor system, composed of a group of organic macromolecules. Cell motors were mapped to an Ising-like model, where the interaction field is transmitted through a tropomyosin polymer chain. The presence of Ca2+ induces tropomyosin to block or unblock binding sites of the myosin motor leading to its activation or deactivation. We used the Metropolis algorithm to find the transient and the equilibrium states of the acto-myosin system composed of solvent, actin, tropomyosin, troponin, Ca2+, and myosin-S1 at a given temperature, including the spatial configuration of tropomyosin on the actin filament surface. Our model describes the short- and long-range cooperativity during actin-myosin binding which emerges from the bending stiffness of the tropomyosin complex. We found all transition rates between the states only using the interaction energy of the constituents. The agreement between our model and experimental data also supports the recent theory of flexible tropomyosin.
Alencar
Adriano M
AM
Department of Environmental Health, Harvard School of Public Health, Boston, Massachusetts 02115, USA.
Butler
James P
JP
Mijailovich
Srboljub M
SM
D000199:Actins / Q000737:chemistry* / Q000378:metabolism*; D000205:Actomyosin / Q000737:chemistry / Q000378:metabolism*; D000465:Algorithms; D002118:Calcium / Q000378:metabolism; D003198:Computer Simulation; D004548:Elasticity; D007700:Kinetics; D008954:Models, Biological; D009010:Monte Carlo Method; D015879:Myosin Subfragments / Q000737:chemistry / Q000378:metabolism*; D011485:Protein Binding / Q000502:physiology; D013816:Thermodynamics; D014335:Tropomyosin / Q000378:metabolism; D014336:Troponin / Q000378:metabolism
D000199:Actins; D015879:Myosin Subfragments; D014335:Tropomyosin; D014336:Troponin; D000205:Actomyosin; D002118:Calcium