title: Influence of some detoxification enzyme polymorphisms on cytogenetic biomarkers between individuals exposed to very low doses of 1,3-butadiene.
abstract: OBJECTIVE
  To evaluate the variation of some biomarkers related to the level of enzymatic activity dependent on the different polymorphisms.
  
  
  METHODS
  We studied 27 butadiene-exposed workers and 37 controls using different biomarkers of the genotoxic effect. The genotypes were determined using polymerase chain reaction and restriction fragment length polymorphism-polymerase chain reaction techniques; the subjects were assigned to a specific group based on the microsomal epoxide hydrolase (mEH) activity predicted by their genotype (low, intermediate, high).
  
  
  RESULTS
  The studied biomarkers were not able to discriminate between exposed and control individuals, but sister chromatid exchange (SCE) and high frequency cells were influenced by smoking habits. Smokers having fast microsomal epoxide hydrolase activity showed higher SCE frequency (7.61) respect to those presenting intermediate (5.86) or slow (6.65) enzymatic activity.
  
  
  CONCLUSIONS
  On the basis of these results, can we suppose the existence of an "intermediate genotype" advantage (at least for induction of SCE)?
authors.affiliation: Department of Internal and Public Medicine, Section of Medical Genetics, University of Bari, Bari, Italy. nenadbukvic@virgilio.it
authors.forename: Nenad
authors.initials: N
authors.lastname: Bukvic
authors.forename: Piero
authors.initials: P
authors.lastname: Lovreglio
authors.forename: Margherita
authors.initials: M
authors.lastname: Fanelli
authors.forename: Francesco C
authors.initials: FC
authors.lastname: Susca
authors.forename: Andrea
authors.initials: A
authors.lastname: Ballini
authors.forename: Patrizia
authors.initials: P
authors.lastname: Lastella
authors.forename: Vito
authors.initials: V
authors.lastname: Foà
authors.forename: Silvia
authors.initials: S
authors.lastname: Fustinoni
authors.forename: Leonardo
authors.initials: L
authors.lastname: Soleo
authors.forename: Ginevra
authors.initials: G
authors.lastname: Guanti
mesh_terms: D000328:Adult; D015415:Biomarkers; D002070:Butadienes / Q000008:administration & dosage / Q000378:metabolism / Q000493:pharmacokinetics* / Q000506:poisoning; D020732:Cytogenetic Analysis; D006801:Humans; D008658:Inactivation, Metabolic; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D009153:Mutagens / Q000008:administration & dosage / Q000378:metabolism / Q000493:pharmacokinetics* / Q000506:poisoning; D016273:Occupational Exposure / Q000009:adverse effects*; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012854:Sister Chromatid Exchange; D055815:Young Adult
chemical_list: D015415:Biomarkers; D002070:Butadienes; D009153:Mutagens; C031763:1,3-butadiene