title: Transfection of normal human epidermal keratinocytes with lipid/dna complexes in vitro.
abstract: Highly proliferative normal human epidermal keratinocytes (NHK) were isolated from human foreskin biopsies, cultivated in serum-free medium and characterized by flow cytometry. The expression of cytokeratin 19, cytokeratin 14 and vimentin indicated that the suspension contained a high percentage of undifferentiated cells of the basal epidermal layer. The NHK were transfected in vitro with lipid/DNA complexes made of Effectene or Lipofectamine and different reporter genes. The transfection efficiency of Effectene/DNA complexes was 20fold higher compared to Lipofectamine. Transfected keratinocytes continued to grow and developed within 2 weeks a cellular multilayer (3-D culture). Areas of transfected cells were detected within this layer.
authors.affiliation: Institute of Biochemistry, Medical Faculty, University Leipzig, Leipzig, D-04103, Germany.
authors.forename: S
authors.initials: S
authors.lastname: Zellmer
authors.forename: A
authors.initials: A
authors.lastname: Surovoy
authors.forename: F
authors.initials: F
authors.lastname: Gaunitz
authors.forename: J
authors.initials: J
authors.lastname: Salvetter
authors.forename: R
authors.initials: R
authors.lastname: Gebhardt
authors.forename: D
authors.initials: D
authors.lastname: Reissig