Dentate granule cells form hilar basal dendrites in a rat model of hypoxia-ischemia.
Hilar basal dendrites form on dentate granule cells following seizures. To determine whether other brain insults cause the formation of hilar basal dendrites, a model of global cerebral hypoxia/ischemia was used. Rats underwent a transient induction of ischemia by occlusion of both common carotid arteries followed by reperfusion. Hippocampal slices were prepared from these animals 1 month after the ischemic insult, and granule cells were labeled with a retrograde tracing technique after biocytin injections into stratum lucidum of CA3b. Ischemic rats had numerous biocytin-labeled granule cells with hilar basal dendrites located at the hilar border of the granule cell layer. Quantitative analysis of ischemic rats compared to controls showed a significant increase in the percentage of biocytin-labeled granule cells with hilar basal dendrites. These data demonstrate that other brain insults in addition to epilepsy may result in the formation of hilar basal dendrites on granule cells.
Díaz-Cintra
Sofia
S
Departamento de Neurobiología del Desarrollo y Neurofisiología, Instituto de Neurobiología, Campus UNAM-Juriquilla, C.P., Mexico.
Xue
Baogang
B
Spigelman
Igor
I
Van
K
K
Wong
Alan M
AM
Obenaus
Andre
A
Ribak
Charles E
CE
D000818:Animals; D020520:Brain Infarction / Q000473:pathology* / Q000503:physiopathology; D003712:Dendrites / Q000473:pathology*; D018891:Dentate Gyrus / Q000473:pathology* / Q000503:physiopathology; D004195:Disease Models, Animal; D020925:Hypoxia-Ischemia, Brain / Q000473:pathology* / Q000503:physiopathology; D008239:Lysine / Q000031:analogs & derivatives; D008297:Male; D009410:Nerve Degeneration / Q000209:etiology / Q000473:pathology* / Q000503:physiopathology; D009434:Neural Pathways / Q000473:pathology / Q000503:physiopathology; D009473:Neuronal Plasticity / Q000502:physiology; D009924:Organ Culture Techniques; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000473:pathology / Q000503:physiopathology; D013194:Staining and Labeling
C013411:biocytin; D008239:Lysine