Activity measurement of chitosanase by an amperometric biosensor.
An amperometric biosensor for the detection of glucosamine (GlcN) and chitosan oligosaccharides ((GlcN)n) was introduced for an activity measurement of chitosanase. By using the biosensor, an increase in the anodic current due to the production of GlcN and (GlcN)n by chitosanase was measured in a chitosan solution. The maximum value of the slope of the current increase was proportional to the enzyme concentration up to 1.4 microg mL(-1). The present method had the advantages of being simple and rapid over the conventional Elson-Morgan method.
Tatsumi
Hirosuke
H
International Young Researchers Empowerment Center, Shinshu University, 3-1-1 Asahi, Matsumoto, Nagano 390-8621, Japan. tatsumi@shinshu-u.ac.jp
Katano
Hajime
H
Ikeda
Tokuji
T
Kimoto
Hisashi
H
D015374:Biosensing Techniques / Q000379:methods*; D048271:Chitosan / Q000032:analysis; D004563:Electrochemistry; D004789:Enzyme Activation; D005944:Glucosamine / Q000032:analysis; D006026:Glycoside Hydrolases / Q000378:metabolism*; D009844:Oligosaccharides / Q000032:analysis
D009844:Oligosaccharides; D048271:Chitosan; D006026:Glycoside Hydrolases; C019868:chitosanase; D005944:Glucosamine