title: Salmonella expressing a T-cell epitope from Sendai virus are able to induce anti-infection immunity.
abstract: Bacterial fimbriae can accept foreign peptides and display them on the cell surface. A highly efficient gene replacement method was used to generate peptide vaccines based on Salmonella enterica subsp. enterica serovar Typhimurium LT2. DNA encoding an epitope from Sendai virus, SV9 (Sendai virus nucleoprotein peptide 324-332, FAPGNYPAL), which is known to induce cytotoxic T lymphocytes, was incorporated into the gene encoding AgfA (the major subunit protein of thin aggregative fimbriae of Salmonella) by replacing an equal length DNA segment. To improve cytotoxic T lymphocyte recognition, both termini of the peptide were flanked by double alanine (AA) or arginine (RR) residues. Western blotting and immunofluorescence microscopy using AgfA-specific antiserum verified the expression of chimeric AgfA; expression was also proved by a Congo red binding assay. Oral immunizations of C57BL/6 mice with the four strains induced an epitope-specific T-cell response (detected by enzyme-linked immunosorbent spot assay). When the mice were challenged with the Sendai virus, the magnitude of the infection was significantly reduced in the immunized groups compared with the controls. The Salmonella fimbrial display system efficiently induces a cellular immune response and anti-infection immunity in vivo, providing a new strategy for the development of efficient peptide vaccination.
authors.affiliation: Department of Microbiology, Peking University Health Science Center, Beijing, PR China.|Laboratory for Immunology and Microbiology, BioMedical Education Center, Peking University Health Science Center, Beijing, PR China.
authors.forename: He
authors.initials: H
authors.lastname: Huang
authors.affiliation: Department of Microbiology, Peking University Health Science Center, Beijing, PR China.
authors.forename: Ye-Jun
authors.initials: YJ
authors.lastname: Wang
authors.affiliation: Department of Microbiology and Infectious Diseases, University of Calgary, Calgary, Canada.
authors.forename: Aaron P
authors.initials: AP
authors.lastname: White
authors.affiliation: Department of Immunology, Peking University Health Science Center, Beijing, PR China.|Laboratory for Immunology and Microbiology, BioMedical Education Center, Peking University Health Science Center, Beijing, PR China.
authors.forename: Jia-Zi
authors.initials: JZ
authors.lastname: Meng
authors.affiliation: Department of Microbiology and Infectious Diseases, University of Calgary, Calgary, Canada.|Department of Microbiology, Peking University Health Science Center, Beijing, PR China.
authors.forename: Gui-Rong
authors.initials: GR
authors.lastname: Liu
authors.affiliation: Genomics Research Center, Harbin Medical University, Harbin 150081, PR China.|Department of Microbiology and Infectious Diseases, University of Calgary, Calgary, Canada.|Department of Microbiology, Peking University Health Science Center, Beijing, PR China.
authors.forename: Shu-Lin
authors.initials: SL
authors.lastname: Liu
authors.affiliation: Department of Immunology, Peking University Health Science Center, Beijing, PR China.|Laboratory for Immunology and Microbiology, BioMedical Education Center, Peking University Health Science Center, Beijing, PR China.
authors.forename: Yue-Dan
authors.initials: YD
authors.lastname: Wang
mesh_terms: D000818:Animals; D000956:Antigens, Viral / Q000235:genetics / Q000276:immunology*; D002642:Chick Embryo; D018984:Epitopes, T-Lymphocyte / Q000235:genetics / Q000378:metabolism*; D005260:Female; D015964:Gene Expression Regulation, Bacterial / Q000235:genetics; D051379:Mice; D008810:Mice, Inbred C57BL; D010253:Respirovirus Infections / Q000517:prevention & control*; D012475:Salmonella / Q000235:genetics / Q000276:immunology*; D012481:Salmonella Infections, Animal / Q000276:immunology / Q000382:microbiology; D029082:Sendai virus / Q000235:genetics* / Q000276:immunology; D013047:Specific Pathogen-Free Organisms; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism; D014765:Viral Vaccines / Q000235:genetics / Q000276:immunology
chemical_list: D000956:Antigens, Viral; D018984:Epitopes, T-Lymphocyte; D014764:Viral Proteins; D014765:Viral Vaccines