Postnatal stress produces hyperglycemia in adult rats exposed to hypoxia-ischemia.
Fetal or early postnatal stressors may predispose infants to develop diabetes, metabolic syndrome, or stroke. We hypothesized that postnatal stress will predispose animals to develop metabolic syndrome and impair the physiologic response to hypoxic-ischemic brain injury. We characterized the short- and long-term physiologic responses to postnatal stress by examining corticosterone (CS), glucose metabolism, and brain injury in neonatal and adult rats exposed to hypoxia-ischemia (H-I). Rat pups were divided into three levels of postnatal stress from postnatal day (P) 3 to P7. All rats underwent unilateral brain injury on either P7 or P134. We measured brain injury, growth, blood pressure, urine/plasma CS, plasma leptin, insulin, and glucose before and after H-I. Postnatal stress increased neonatal CS production, exacerbated neonatal white matter injury, and was associated with adult hyperglycemia after H-I despite increased insulin production. There were no group differences in adult weight, blood pressure, or leptin. Postnatal stress exacerbated brain injury and produced adult hyperglycemia, triggered after hypoxia exposure, consistent with the hypotheses that neonates exposed to early stress are more vulnerable to hypoxia and may be predisposed to develop metabolic syndrome in adulthood. Prolonged maternal separation produced more hyperglycemia than did brief daily handling.
McPherson
Ronald J
RJ
Department of Pediatrics, University of Washington, Seattle, Washington 98195, USA.
Mascher-Denen
Marcella
M
Juul
Sandra E
SE
D000818:Animals; D000831:Animals, Newborn / Q000502:physiology; D001786:Blood Glucose / Q000378:metabolism; D001794:Blood Pressure / Q000502:physiology; D001835:Body Weight; D001930:Brain Injuries / Q000378:metabolism / Q000473:pathology; D003345:Corticosterone / Q000097:blood / Q000652:urine; D005260:Female; D006801:Humans; D006943:Hyperglycemia / Q000378:metabolism*; D020925:Hypoxia-Ischemia, Brain / Q000503:physiopathology*; D007328:Insulin / Q000097:blood; D020738:Leptin / Q000097:blood; D011247:Pregnancy; D051381:Rats; D017207:Rats, Sprague-Dawley; D013312:Stress, Physiological
D001786:Blood Glucose; D007328:Insulin; D020738:Leptin; D003345:Corticosterone