title: [T cell receptor diversity of CD8+ T lymphocytes and its association with viral load in individuals with HIV-1 infection].
abstract: OBJECTIVE
  To determine the complementary determining region 3 (CDR3) length diversity of T cell receptor Vbeta repertoires of CD8+ T lymphocytes and to explore its association with viral load in individuals with HIV-1 infection.
  
  
  METHODS
  Separation of CD8+ T cells from peripheral blood mononuclear cells (PBMCs) was carried out by using immunomagnetic beads coated with anti-CD8 antibody. Total RNAs from the purified CD8+ T lymphocytes were isolated and used to perform polymerase chain reaction (PCR) amplifications in CDR3 of 22 T cell receptor (TCR) gene families. CDR3 diversity and its association with viral load in individuals with HIV-1 infection were analyzed.
  
  
  RESULTS
  An average diversity for all CDR3 profiles in CD8+ T cells from 9 HIV-infected individuals was significantly different as compared to 7 age-matched healthy donors (P<0.05) with the HIV-infected individuals losing diversity in the CDR3 profiles. There was positive correlation between changes in TCR CDR3 diversity and viral load (r=0.771, P<0.05). The changes in CDR3 length diversity of Vbeta families in HIV-infected individuals, particular in Vbeta2, Vbeta4, Vbeta5, Vbeta17, Vbeta20, Vbeta21, Vbeta23, Vbeta24, were statistically different from the healthy controls.
  
  
  CONCLUSIONS
  HIV-1 infection might induce the loss of TCR Vbeta repertoire diversity and disrupt the CDR3 distributions within CD8+ T cells. There should be positive correlation between changes in TCR CDR3 diversity and the viral load in HIV-1 infected patients.
authors.affiliation: National Center for AIDS/STD Control and Prevention, Chinese Center for Disease Control and Prevention, State Key Laboratory for Infectious Disease Prevention and Control, Beijing 100050, China.
authors.forename: Guo-liang
authors.initials: GL
authors.lastname: Ren
authors.forename: Jian-ping
authors.initials: JP
authors.lastname: Chen
authors.forename: Ming-ming
authors.initials: MM
authors.lastname: Jia
authors.forename: Zhong-chen
authors.initials: ZC
authors.lastname: Kou
authors.forename: Sha
authors.initials: S
authors.lastname: Liu
authors.forename: Peng-fei
authors.initials: PF
authors.lastname: Ma
authors.forename: Yi-ming
authors.initials: YM
authors.lastname: Shao
authors.forename: Kun-xue
authors.initials: KX
authors.lastname: Hong
mesh_terms: D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D015658:HIV Infections / Q000235:genetics* / Q000821:virology*; D015497:HIV-1 / Q000276:immunology; D006801:Humans; D011110:Polymorphism, Genetic; D011948:Receptors, Antigen, T-Cell / Q000235:genetics*; D019562:Viral Load
chemical_list: D011948:Receptors, Antigen, T-Cell