Genomic structure of the whole D-J-C clusters and the upstream region coding V segments of the TRB locus in pig.
In the vertebrate immune system, T cells play a central role in host defense against microbial or viral infection. Previous studies suggested that at least two sets of TRBD-J-C clusters are harbored in the porcine genome. In this study, we determined 212,193 bp of a continuous porcine genomic sequence covering the entire TRBC region. EPHB6, TRPV6, TRY, and ten TRBV genes were conserved in the vicinity of the TRBD-J-C clusters. Interestingly, three TRBD-J-C clusters were identified in this sequence; each TRBD-J-C cluster consisted of one TRBD and seven TRBJ segments, with one TRBC region composed of four exons. The distribution of repetitive sequences and phylogenetic analysis indicated that the TRBD-J-C cluster, located at the center of the three clusters identified, had a structure combined with the others. Most of the TRBJ segments were available in public databases, suggesting that all three TRBD-J-C clusters are functional in pigs.
Eguchi-Ogawa
Tomoko
T
Division of Animal Sciences, National Institute of Agrobiological Sciences (NIAS), 2-1-2 Kannondai, Tsukuba, Ibaraki 305-8602, Japan.
Toki
Daisuke
D
Uenishi
Hirohide
H
D000818:Animals; D001188:Artiodactyla / Q000235:genetics; D022202:Chromosomes, Artificial, Bacterial / Q000235:genetics; D003001:Cloning, Molecular; D019674:Genes, T-Cell Receptor beta / Q000235:genetics; D016678:Genome / Q000235:genetics*; D020071:Interspersed Repetitive Sequences / Q000235:genetics; D005810:Multigene Family; D010802:Phylogeny; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000145:classification / Q000235:genetics*; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000145:classification / Q000235:genetics*; D017422:Sequence Analysis, DNA; D013552:Swine / Q000235:genetics*
D016693:Receptors, Antigen, T-Cell, alpha-beta; D016692:Receptors, Antigen, T-Cell, gamma-delta