Vaccinia virus CD8+ T-cell dominance hierarchies cannot be altered by prior immunization with individual peptides.
Heterologous prime-boost is a common vaccination strategy to elicit CD8(+) T cells (T(CD8+)), and vaccinia virus (VACV) has been widely used as a boosting vector. Studies with other viruses have suggested that priming may reduce responses to native epitopes in boosting vectors as well as improve responses to primed epitopes. We explored this possibility with a VACV model in mice and find that irrespective of an epitope's dominance, prior priming was able to double T(CD8+) responses. More surprisingly, and in contrast to findings for other viruses, responses to remaining epitopes were undisturbed, leaving the overall dominance hierarchy unchanged.
Wang
Yang
Y
Department of Biochemistry & Molecular Biology, Research School of Biology, College of Medicine, Biology and the Environment, Australian National University, Canberra, ACT, Australia.
Flesch
Inge E A
IE
Tscharke
David C
DC
D000818:Animals; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D018984:Epitopes, T-Lymphocyte / Q000276:immunology*; D007117:Immunization, Secondary; D016056:Immunodominant Epitopes / Q000276:immunology*; D007371:Interferon-gamma / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D010446:Peptide Fragments / Q000378:metabolism; D014616:Vaccinia virus / Q000276:immunology*
D018984:Epitopes, T-Lymphocyte; D016056:Immunodominant Epitopes; D010446:Peptide Fragments; C069261:interferon gamma (1-39); D007371:Interferon-gamma