title: Tigecycline susceptibility report from an Indian tertiary care hospital.
abstract: OBJECTIVE
  Treatment of serious life threatening infections due to multi-drug resistant pathogens presents a difficult challenge due to the limited therapeutic options. Therefore, we studied the in vitro susceptibility of tigecycline, a new glycylcycline with promising broad spectrum of activity against Gram positive and Gram negative bacteria at a tertiary care hospital in north India.
  
  
  METHODS
  A total of 75 multi-drug resistant isolates of methicillin resistant Staphylococcus aureus (21), vancomycin resistant enterococci (14), vancomycin resistant Streptococcus spp. (3), extended spectrum beta lactamase producing Gram negative bacteria (11) and multi-resistant Acinetobacter spp. (26) were tested for tigecycline susceptibility by the E-test and disc diffusion methods. An additional 83 multi-resistant Gram negative clinical isolates were screened by disc diffusion method alone.
  
  
  RESULTS
  All the isolates of MRSA, VRE, vancomycin resistant Streptococcus spp. and ESBL producing enteric bacteria were sensitive to tigecycline by the E-test and disc diffusion methods. However, only 42 per cent of Acinetobacter spp. were found to be sensitive to tigecycline by the E-test method.
  
  
  CONCLUSIONS
  In conclusion, tigecycline was found to be highly effective against Gram-positive bacteria and Gram-negative members of Enterobacteriaceae, but a high prevalence of resistance in members of Acinetobacter spp. is worrisome.
authors.affiliation: Department of Laboratory Medicine, Jai Prakash Narayan Apex Trauma Centre, New Delhi, India.
authors.forename: Bijayini
authors.initials: B
authors.lastname: Behera
authors.forename: Anupam
authors.initials: A
authors.lastname: Das
authors.forename: Purva
authors.initials: P
authors.lastname: Mathur
authors.forename: Arti
authors.initials: A
authors.lastname: Kapil
authors.forename: Ravisekhar
authors.initials: R
authors.lastname: Gadepalli
authors.forename: Benu
authors.initials: B
authors.lastname: Dhawan
mesh_terms: D000150:Acinetobacter / Q000187:drug effects*; D000900:Anti-Bacterial Agents / Q000633:toxicity*; D052978:Disk Diffusion Antimicrobial Tests; D024901:Drug Resistance, Multiple, Bacterial; D006761:Hospitals; D066298:In Vitro Techniques; D008911:Minocycline / Q000031:analogs & derivatives* / Q000633:toxicity; D013045:Species Specificity; D013211:Staphylococcus aureus / Q000187:drug effects*; D013291:Streptococcus / Q000187:drug effects*; D000078304:Tigecycline
chemical_list: D000900:Anti-Bacterial Agents; D000078304:Tigecycline; D008911:Minocycline