Human fallopian tube: a new source of multipotent adult mesenchymal stem cells discarded in surgical procedures.
BACKGROUND
The possibility of using stem cells for regenerative medicine has opened a new field of investigation. The search for sources to obtain multipotent stem cells from discarded tissues or through non-invasive procedures is of great interest. It has been shown that mesenchymal stem cells (MSCs) obtained from umbilical cords, dental pulp and adipose tissue, which are all biological discards, are able to differentiate into muscle, fat, bone and cartilage cell lineages. The aim of this study was to isolate, expand, characterize and assess the differentiation potential of MSCs from human fallopian tubes (hFTs).


METHODS
Lineages of hFTs were expanded, had their karyotype analyzed, were characterized by flow cytometry and underwent in vitro adipogenic, chondrogenic, osteogenic, and myogenic differentiation.


RESULTS
Here we show for the first time that hFTs, which are discarded after some gynecological procedures, are a rich additional source of MSCs, which we designated as human tube MSCs (htMSCs).


CONCLUSIONS
Human tube MSCs can be easily isolated, expanded in vitro, present a mesenchymal profile and are able to differentiate into muscle, fat, cartilage and bone in vitro.
Jazedje
Tatiana
T
Human Genome Research Center, Biosciences Institute, University of São Paulo, n degrees 106, Cidade Universitária São Paulo SP, CEP: 05508-090, Brazil. tatiana@ib.usp.br
Perin
Paulo M
PM
Czeresnia
Carlos E
CE
Maluf
Mariangela
M
Halpern
Silvio
S
Secco
Mariane
M
Bueno
Daniela F
DF
Vieira
Natassia M
NM
Zucconi
Eder
E
Zatz
Mayana
M
D017667:Adipocytes / Q000166:cytology; D000328:Adult; D002454:Cell Differentiation / Q000502:physiology; D019070:Cell Lineage; D049109:Cell Proliferation; D002469:Cell Separation; D002478:Cells, Cultured; D019902:Chondrocytes / Q000166:cytology; D005187:Fallopian Tubes / Q000166:cytology* / Q000601:surgery; D005260:Female; D006801:Humans; D007621:Karyotyping; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000502:physiology; D039902:Multipotent Stem Cells / Q000166:cytology*; D032342:Muscle Cells / Q000166:cytology; D010006:Osteoblasts / Q000166:cytology