In vivo indirect measurement of cytochrome P450-associated activities in freshwater gastropod molluscs.
The cytochrome P450 (CYP) system is widely distributed across phyla and plays a key role in the metabolism of xenobiotic compounds. However, most studies on CYP system were developed on vertebrates and among invertebrates, gastropod molluscs are rarely used. In this context, ethoxycoumarin-O-deethylase (ECOD), ethoxyresorufin-O-deethylase (EROD), and pentoxyresorufin-O-dealkylase (PROD) activities, which are indirect measurements of CYP system, were characterized in two freshwater gastropod molluscs, Potamopyrgus antipodarum, and Valvata piscinalis, to ascertain their potential interest as biomarkers of exposure to chemicals. Activities were measured using an in vivo non lethal method based on the measurement of formed product (resorufin or hydroxycoumarin). This in vivo assay allowed to measure the three activities in P. antipodarum and two of them (ECOD and PROD) in V. piscinalis. The detection of activities and the optimization of experimental design were carried out first and allowed to measure the selected activities for one individual. The modulation of the detected activities was secondly assessed using a polycyclic aromatic hydrocarbon (Benzo(a)pyrene). Based on this non destructive measurement, effect of BaP exposure could be detected on ECOD and EROD activity in P. antipodarum, as well on PROD activity of V. piscinalis after 96 h of exposure. Such an in vivo assay must be further developed to be valuably used to screen the exposure of gastropod species to CYP inducer chemicals and its consequences in terms of fitness of the organisms and of the population.
Gagnaire
Beatrice
B
CEMAGREF, Unité Biologie des Ecosystèmes Aquatiques, 3 bis quai Chauveau, CP 220, 69336 Lyon Cedex 09, France.
Geffard
Olivier
O
Noury
Patrice
P
Garric
Jeanne
J
D015660:7-Alkoxycoumarin O-Dealkylase / Q000032:analysis; D000818:Animals; D001564:Benzo(a)pyrene / Q000378:metabolism; D003374:Coumarins / Q000378:metabolism; D019363:Cytochrome P-450 CYP1A1 / Q000032:analysis; D019362:Cytochrome P-450 CYP2B1 / Q000032:analysis; D003577:Cytochrome P-450 Enzyme System / Q000032:analysis*; D049851:Gastropoda / Q000201:enzymology*; D006899:Mixed Function Oxygenases / Q000032:analysis*; D010078:Oxazines / Q000378:metabolism; D014468:Umbelliferones / Q000378:metabolism
D003374:Coumarins; D010078:Oxazines; D014468:Umbelliferones; C017299:7-ethoxycoumarin; D001564:Benzo(a)pyrene; C007649:ethoxyresorufin; C031477:7-hydroxycoumarin; C014180:resorufin; C044943:pentoxyresorufin; D003577:Cytochrome P-450 Enzyme System; D006899:Mixed Function Oxygenases; D015660:7-Alkoxycoumarin O-Dealkylase; D019363:Cytochrome P-450 CYP1A1; D019362:Cytochrome P-450 CYP2B1