title: An alternative method of promoter assessment by confocal laser scanning microscopy.
abstract: A rapid and useful method of promoter activity analysis using techniques of confocal laser scanning microscopy (CLSM) is described in the present study. The activities of some pararetroviral promoters such as CaMV35S (Cauliflower mosaic virus), FMVSgt3 (Figwort mosaic virus sub-genomic transcript) and MMVFLt12 (Mirabilis mosaic virus full-length transcript) coupled to GFP (green fluorescent protein) and GUS (beta-glucuronidase) reporter genes were determined simultaneously by the CLSM technique and other available conventional methods for reporter gene assay based on relevant biochemical and molecular approaches. Consistent and comparable results obtained by CLSM as well as by other conventional assay methods confirm the effectiveness of the CLSM approach for assessment of promoter activity. Hence the CLSM method can be suggested as an alternative way for promoter analysis on the basis of high throughput.
authors.affiliation: Institute of Life Sciences, Nalco Square, Chandrasekhar Pur, Bhubaneswar, Orissa, India.
authors.forename: Dipak K
authors.initials: DK
authors.lastname: Sahoo
authors.forename: Rajiv
authors.initials: R
authors.lastname: Ranjan
authors.forename: Deepak
authors.initials: D
authors.lastname: Kumar
authors.forename: Alok
authors.initials: A
authors.lastname: Kumar
authors.forename: Bhabani S
authors.initials: BS
authors.lastname: Sahoo
authors.forename: Sumita
authors.initials: S
authors.lastname: Raha
authors.forename: Indu B
authors.initials: IB
authors.lastname: Maiti
authors.forename: Nrisingha
authors.initials: N
authors.lastname: Dey
mesh_terms: D019677:Artificial Gene Fusion; D017796:Caulimovirus / Q000235:genetics*; D015870:Gene Expression; D017930:Genes, Reporter; D005966:Glucuronidase / Q000235:genetics / Q000378:metabolism; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D018613:Microscopy, Confocal / Q000379:methods*; D011401:Promoter Regions, Genetic
chemical_list: C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins; D005966:Glucuronidase