title: AKAP-independent localization of type-II protein kinase A to dynamic actin microspikes.
abstract: Regulation of the cyclic AMP-dependent protein kinase (PKA) in subcellular space is required for cytoskeletal dynamics and chemotaxis. Currently, spatial regulation of PKA is thought to require the association of PKA regulatory (R) subunits with A-kinase anchoring proteins (AKAPs). Here, we show that the regulatory RIIalpha subunit of PKA associates with dynamic actin microspikes in an AKAP-independent manner. Both endogenous RIIalpha and a GFP-RIIalpha fusion protein co-localize with F-actin in microspikes within hippocampal neuron growth cones and the leading edge lamellae of NG108-15 cells. Live-cell imaging demonstrates that RIIalpha-associated microspikes are highly dynamic and that the coupling of RIIalpha to actin is tight, as the movement of both actin and RIIalpha are immediately and coincidently stopped by low-dose cytochalasin D. Importantly, co-localization of RIIalpha and actin in these structures is resistant to displacement by a cell-permeable disrupter of PKA-AKAP interactions. Biochemical fractionation confirms that a substantial pool of PKA RIIalpha is associated with the detergent-insoluble cytoskeleton and is resistant to extraction by a peptide inhibitor of AKAP interactions. Finally, mutation of the AKAP-binding domain of RIIalpha fails to disrupt its association with actin microspikes. These data provide the first demonstration of the physical association of a kinase with such dynamic actin structures, as well as the first demonstration of the ability of type-II PKA to localize to discrete subcellular structures independently of canonical AKAP function. This association is likely to be important for microfilament dynamics and cell migration and may prime the investigation of novel mechanisms for localizing PKA activity.
authors.affiliation: Department of Pharmacology, University of Vermont College of Medicine, Burlington, VT 05405, USA.
authors.forename: Robert L
authors.initials: RL
authors.lastname: Rivard
authors.forename: Monique
authors.initials: M
authors.lastname: Birger
authors.forename: Kara J
authors.initials: KJ
authors.lastname: Gaston
authors.forename: Alan K
authors.initials: AK
authors.lastname: Howe
mesh_terms: D054758:A Kinase Anchor Proteins / Q000187:drug effects / Q000378:metabolism*; D000199:Actins / Q000187:drug effects / Q000378:metabolism*; D000818:Animals; D045744:Cell Line, Tumor; D054746:Cyclic AMP-Dependent Protein Kinase Type II / Q000187:drug effects / Q000378:metabolism*; D015638:Cytochalasin D / Q000494:pharmacology; D003599:Cytoskeleton / Q000187:drug effects / Q000201:enzymology*; D051379:Mice; D009474:Neurons / Q000166:cytology / Q000187:drug effects / Q000201:enzymology*; D019384:Nucleic Acid Synthesis Inhibitors / Q000494:pharmacology; D051381:Rats
chemical_list: D054758:A Kinase Anchor Proteins; D000199:Actins; D019384:Nucleic Acid Synthesis Inhibitors; D015638:Cytochalasin D; D054746:Cyclic AMP-Dependent Protein Kinase Type II