title: [Isolation and primary identification of viruses in mosquitoes in the south of Xinjiang].
abstract: OBJECTIVE
  To isolate viruses from mosquitoes in the south of Xinjiang and identify these viruses primarily.
  
  
  METHODS
  A total of 13 491 mosquitoes were collected in the south of Xinjiang from Jul to Aug, 2005. These mosquitoes were divided into 130 groups and grinded respectively. The supernates were inoculated in C6/36 and Vero cells. Viruses isolated were detected, the genomic nucleic types by electrophoresis of viral genomes and the morphologies observed under electronmicroscope.
  
  
  RESULTS
  All 42 viruses were isolated, which caused CPEs on C6/36 but not on Vero cells. 27 viruses showed similar genomic profiles with 12 dsRNA segments. 1 virus displayed genomic profile with 10 dsRNA segments. 5 viruses took on similar genomic profiles with about 4 kbp DNA band. 9 viruses did not get any taxonomy information. Electromicroscopic pictures of these viruses revealed that above four types of viruses had distinguished morphologies indicating different virus species.
  
  
  CONCLUSIONS
  There should be several virus species in the mosquitoes in the south of Xinjiang. dsRNA virus with 12 genomic segments should play analysis a predominant role in the south of Xinjiang.
authors.affiliation: Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 100052, China.
authors.forename: Xin-jun
authors.initials: XJ
authors.lastname: Lü
authors.forename: Zhi
authors.initials: Z
authors.lastname: Lü
authors.forename: Xiao-hong
authors.initials: XH
authors.lastname: Sun
authors.forename: Shi-hong
authors.initials: SH
authors.lastname: Fu
authors.forename: Huan-qin
authors.initials: HQ
authors.lastname: Wang
authors.forename: Su-xiang
authors.initials: SX
authors.lastname: Tong
authors.forename: Song
authors.initials: S
authors.lastname: Zhang
authors.forename: Guo-dong
authors.initials: GD
authors.lastname: Liang
mesh_terms: D000818:Animals; D001820:Bluetongue virus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D002681:China; D002522:Chlorocebus aethiops; D009033:Culicidae / Q000821:virology*; D003716:Dengue Virus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D016679:Genome, Viral; D007304:Insect Viruses / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D012330:RNA, Double-Stranded / Q000235:genetics; D012367:RNA, Viral / Q000235:genetics; D016865:Reassortant Viruses / Q000235:genetics; D017422:Sequence Analysis, DNA; D014709:Vero Cells
chemical_list: D012330:RNA, Double-Stranded; D012367:RNA, Viral