title: Fabrication of porous gelatin microfibers using an aqueous wet spinning process.
abstract: Alginate has a unique property of gel formation by chelating with divalent cations such as Ca(+2) in aqueous solution. The sol-gel characteristic of alginate has been utilized to fabricate both microsphere and microcapsule for cell immobilization. In this study, a wet spinning process was employed to prepare fibers comprised of gelatin and sodium alginate. Gelatin fibers containing interconnected porous structure were fabricated by the extraction of alginate from these gelatin/alginate composite fibers with phosphate buffer. The application of these porous gelatin fibers for enzyme immobilization was demonstrated by its usage as the base material of glucose biosensor.
authors.affiliation: Institute of Biomedical Engineering, National Yang Ming University, Taipei, Taiwan, Republic of China.
authors.forename: Chen Y
authors.initials: CY
authors.lastname: Yang
authors.forename: Chih T
authors.initials: CT
authors.lastname: Chiu
authors.forename: Yi P
authors.initials: YP
authors.lastname: Chang
authors.forename: Yng J
authors.initials: YJ
authors.lastname: Wang
mesh_terms: D000464:Alginates / Q000737:chemistry / Q000302:isolation & purification; D004566:Electrodes; D004800:Enzymes, Immobilized / Q000737:chemistry; D005780:Gelatin / Q000138:chemical synthesis* / Q000737:chemistry*; D005947:Glucose / Q000737:chemistry; D020723:Glucuronic Acid / Q000737:chemistry / Q000302:isolation & purification; D006603:Hexuronic Acids / Q000737:chemistry / Q000302:isolation & purification; D016062:Porosity; D012399:Rotation; D014867:Water / Q000737:chemistry*
chemical_list: D000464:Alginates; D004800:Enzymes, Immobilized; D006603:Hexuronic Acids; D014867:Water; D020723:Glucuronic Acid; D005780:Gelatin; D005947:Glucose