Inhibition of 11beta-hydroxysteroid dehydrogenase type 1 by plant extracts used as traditional antidiabetic medicines.
Elevated glucocorticoids are a key risk factor for metabolic diseases, and the glucocorticoid-activating enzyme 11beta-hydroxysteroid dehydrogenase 1 (11beta-HSD1) represents a promising therapeutic target. We measured the potential of six traditional antidiabetic medicinal plants extracts to inhibit 11beta-HSD1 activity and glucocorticoid receptor (GR) activation in transfected HEK-293 cells. Leave extracts of Eriobotrya japonica preferentially inhibited 11beta-HSD1 over 11beta-HSD2. Extracts of roasted but not native coffee beans preferentially inhibited 11beta-HSD1 over 11beta-HSD2, emphasizing the importance of sample preparation. Thus, natural compounds inhibiting 11beta-HSD1 may contribute to the antidiabetic effect of the investigated plant extracts.
Gumy
Christel
C
Division of Molecular and Systems Toxicology, Department of Pharmaceutical Sciences, University of Basel, Klingelbergstrasse 50, CH-4056 Basel, Switzerland. christel.gumy@gmail.com
Thurnbichler
Carmen
C
Aubry
Evelyne M
EM
Balazs
Zoltan
Z
Pfisterer
Petra
P
Baumgartner
Lisa
L
Stuppner
Hermann
H
Odermatt
Alex
A
Rollinger
Judith M
JM
D043205:11-beta-Hydroxysteroid Dehydrogenase Type 1 / Q000037:antagonists & inhibitors*; D002460:Cell Line; D040503:Coffea; D031983:Eriobotrya; D006801:Humans; D007004:Hypoglycemic Agents / Q000494:pharmacology*; D008519:Medicine, Traditional; D010936:Plant Extracts / Q000494:pharmacology*; D018515:Plant Leaves; D011965:Receptors, Glucocorticoid / Q000037:antagonists & inhibitors*; D012639:Seeds
D007004:Hypoglycemic Agents; D010936:Plant Extracts; D011965:Receptors, Glucocorticoid; D043205:11-beta-Hydroxysteroid Dehydrogenase Type 1