The mycobacterial glycolipid glucose monomycolate induces a memory T cell response comparable to a model protein antigen and no B cell response upon experimental vaccination of cattle.
Glycolipids are presented to T cells by human group 1 CD1 proteins, but are not used as subunit vaccines yet. Experimental immunizations with pure mycobacterial glucose monomycolate (GMM) and keyhole limpet haemocyanin (KLH) in cattle, a species which, unlike mice, expresses group 1 CD1, showed that GMM was equally efficient as KLH in generating T cell responses in blood, but not in the draining lymph node. Also, KLH induced strong antibody responses whereas GMM did not. These data suggest that non-overlapping T cell populations are targeted and demonstrate the potential of glycolipids as a special class of subunit vaccine candidates.
Nguyen
Thi Kim Anh
TK
Department of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, Utrecht University, Yalelaan 1, Utrecht, The Netherlands.
Koets
Ad P
AP
Santema
Wiebren J
WJ
van Eden
Willem
W
Rutten
Victor P M G
VP
Van Rhijn
Ildiko
I
D000276:Adjuvants, Immunologic / Q000008:administration & dosage / Q000494:pharmacology; D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology; D002417:Cattle; D006017:Glycolipids / Q000276:immunology* / Q000302:isolation & purification; D006433:Hemocyanins / Q000276:immunology; D007156:Immunologic Memory; D009163:Mycobacterium bovis / Q000737:chemistry* / Q000276:immunology; D000644:Quaternary Ammonium Compounds / Q000008:administration & dosage / Q000494:pharmacology; D013601:T-Lymphocytes / Q000276:immunology*; D032581:Tuberculosis Vaccines / Q000276:immunology*
D000276:Adjuvants, Immunologic; D000907:Antibodies, Bacterial; D006017:Glycolipids; D000644:Quaternary Ammonium Compounds; D032581:Tuberculosis Vaccines; C048938:glucose mycolate; C015831:dimethyldioctadecylammonium; D006433:Hemocyanins; C032808:keyhole-limpet hemocyanin