title: [Label-free quantitative analysis of proteins using mass-spectrometry].
abstract: In the present paper we highlight the dependence of normalized mass spectrometry intensity on protein concentration. We have defined dynamic range of concentrations from 1 nM to 1 mcM. It has been shown that the dependence holds linearity along the whole concentration range as for pure proteins as for proteins mixtures. Our observation revealed that the correlation curve possess protein-specific tangent between 1 nM and 1 mcM. The efficacy of the method evaluated by AQUA and 18O-labelling techniques of quantitative proteomics.
authors.forename: A T
authors.initials: AT
authors.lastname: Kopylov
authors.forename: V G
authors.initials: VG
authors.lastname: Zgoda
authors.forename: A I
authors.initials: AI
authors.lastname: Archakov
mesh_terms: D000818:Animals; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods*; D011506:Proteins / Q000032:analysis* / Q000737:chemistry; D040901:Proteomics / Q000379:methods*; D012680:Sensitivity and Specificity
chemical_list: D011506:Proteins