Synthesis and properties of a novel molecular beacon containing a benzene-phosphate backbone at its stem moiety.
This paper describes the synthesis and properties of a novel molecular beacon (MB) containing a benzene-phosphate backbone at its stem moiety. The fluorescence intensity of MBs was found to stabilize by the introduction of the benzene-phosphate backbone at its stem moiety. Furthermore, an MB containing the benzene-phosphate backbone was more resistant to DNase I (endonuclease) than an MB comprising natural DNA and 2'-O-methyl-RNA. These results indicate that the MB with the benzene-phosphate backbone is superior as a molecular beacon as compared to the MB composed of natural DNA and 2'-O-methyl-RNA.
Ueno
Yoshihito
Y
Department of Biomolecular Science, Faculty of Engineering, Gifu University, Japan. uenoy@gifu-u.ac.jp
Kawamura
Akihiro
A
Takasu
Keiji
K
Komatsuzaki
Shinji
S
Kato
Takumi
T
Kuboe
Satoru
S
Kitamura
Yoshiaki
Y
Kitade
Yukio
Y
D001555:Benzene Derivatives / Q000138:chemical synthesis* / Q000737:chemistry*; D004247:DNA / Q000737:chemistry; D003850:Deoxyribonuclease I / Q000737:chemistry / Q000378:metabolism; D005453:Fluorescence; D006860:Hydrogen Bonding; D015394:Molecular Structure; D010710:Phosphates / Q000737:chemistry*; D012313:RNA / Q000737:chemistry; D013696:Temperature; D013997:Time Factors
D001555:Benzene Derivatives; D010710:Phosphates; D012313:RNA; D004247:DNA; D003850:Deoxyribonuclease I