Regulation of the anaphase-promoting complex by the COP9 signalosome.
The COP9 complex (signalosome) is a known regulator of the proteasome/ubiquitin pathway. Furthermore it regulates the activity of the cullin-RING ligase (CRL) families of ubiquitin E3-complexes. Besides the CRL family, the anaphase-promoting complex (APC/C) is a major regulator of the cell cycle. To investigate a possible connection between both complexes we assessed interacting partners of COP9 using an in vivo protein-protein interaction assay. Hereby, we were able to show for the first time that CSN2, a subunit of the COP9 signalosome, interacts physically with APC/C. Furthermore, we detected a functional influence of the COP9 complex regarding the stability of several targets of the APC/C. Consistent with these data we showed a genetic instability of cells overexpressing CSN2.
Kob
Robert
R
Core Unit Chip Application, Institute of Human Genetics and Anthropology, Jena University Hospital, Jena, Germany.
Kelm
Juliane
J
Posorski
Nicole
N
Baniahmad
Aria
A
von Eggeling
Ferdinand
F
Melle
Christian
C
D064173:Anaphase-Promoting Complex-Cyclosome; D017209:Apoptosis; D000075686:COP9 Signalosome Complex; D045744:Cell Line, Tumor; D042822:Genomic Instability; D006801:Humans; D046912:Multiprotein Complexes / Q000378:metabolism*; D010447:Peptide Hydrolases / Q000378:metabolism*; D012097:Repressor Proteins / Q000378:metabolism*; D043743:Ubiquitin-Protein Ligase Complexes / Q000378:metabolism*
C402762:COPS2 protein, human; D046912:Multiprotein Complexes; D012097:Repressor Proteins; D043743:Ubiquitin-Protein Ligase Complexes; D064173:Anaphase-Promoting Complex-Cyclosome; D010447:Peptide Hydrolases; D000075686:COP9 Signalosome Complex