title: Hepatitis B virus genetic typing using mass-spectrometry.
abstract: Mini-sequencing with subsequent result registration using MALDI-ToF mass-spectrometry was employed for hepatitis B virus genetic typing in Russian population. This approach was employed for hepatitis B virus genetic typing in HBsAg-positive patients with chronic hepatitis B, hepatitis of combined etiology and hepatic cirrhosis and allowed to show the prevalence of D genotype (83.3%) in all groups of patients. Other hepatitis B virus genotypes: genotype A (5.9%), genotype C (3.6%), and mixed infection with D and C (7.2%) were also found in patients with chronic hepatitis B and hepatic cirrhosis. All genotypes were found in patients with chronic hepatitis B and hepatic cirrhosis. Chronic hepatitis of combined etiology was noted only in patients with genotype D. Possibility of detection of mixed infection with hepatitis B viruses of various genotypes is a distinct advantage of mini-sequencing approach over direct nucleotide sequence evaluation for hepatitis B virus genetic typing.
authors.affiliation: Scientific Research Institute of Physical-Chemical Medicine, Moscow State Medical-Stomatological University, Moscow, Russia. maja_m@mail.ru
authors.forename: M V
authors.initials: MV
authors.lastname: Malakhova
authors.forename: E N
authors.initials: EN
authors.lastname: Ilina
authors.forename: V M
authors.initials: VM
authors.lastname: Govorun
authors.forename: S A
authors.initials: SA
authors.lastname: Shutko
authors.forename: K R
authors.initials: KR
authors.lastname: Dudina
authors.forename: O O
authors.initials: OO
authors.lastname: Znoyko
authors.forename: E A
authors.initials: EA
authors.lastname: Klimova
authors.forename: N D
authors.initials: ND
authors.lastname: Iushchuk
mesh_terms: D001483:Base Sequence; D005838:Genotype; D006509:Hepatitis B / Q000821:virology; D006515:Hepatitis B virus / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D008828:Microbiological Techniques / Q000379:methods*; D008969:Molecular Sequence Data; D012689:Sequence Homology, Nucleic Acid; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization