title: Characterization and influences of classical insect hormones on the expression profiles of a molting carboxypeptidase A from the cotton bollworm (Helicoverpa armigera).
abstract: Molting is a very important physiological behavior to arthropods. During molting, integument apolysis occurs, which is the digestion and absorption of the old endocuticle for new cuticle formation. Proteases play critical roles in this process. Molting carboxypeptidase A (Ha-CPA) is characterized from Helicoverpa armigera. The Ha-CPA transcript was mainly present in the integument from the 5th instar larvae. In the integument, the transcription level of the gene reached its peak at the 5th instar molting stage and the 6th instar prepupal stage, respectively. The examination of immunohistochemistry revealed that Ha-CPA could distribute into the molting fluid in the molting- and prepupal-stage larvae. The expression of Ha-CPA could be up-regulated by 20-hydroxyecdysone (20E). These facts indicate that Ha-CPA participates in the apolysis of the integument during larval molting and metamorphosis.
authors.affiliation: Shandong University, Jinan, China.
authors.forename: Y-P
authors.initials: YP
authors.lastname: Sui
authors.forename: X-B
authors.initials: XB
authors.lastname: Liu
authors.forename: L-Q
authors.initials: LQ
authors.lastname: Chai
authors.forename: J-X
authors.initials: JX
authors.lastname: Wang
authors.forename: X-F
authors.initials: XF
authors.lastname: Zhao
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D043422:Carboxypeptidases A / Q000235:genetics / Q000378:metabolism*; D003001:Cloning, Molecular; D020869:Gene Expression Profiling; D007150:Immunohistochemistry; D007605:Juvenile Hormones / Q000378:metabolism*; D008675:Metamorphosis, Biological; D008969:Molecular Sequence Data; D009036:Moths / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development
chemical_list: D007605:Juvenile Hormones; D043422:Carboxypeptidases A