Generation and characterization of monoclonal antibodies specific to Drosophila presenilin.
The development of a monoclonal antibody (MAb) specific to Drosophila presenilin (Psn) proteins in vivo was the major aim of this study, since the absence of specific antibodies recognizing Psn proteins hampered our progress in understanding Psn functions during development, differentiation, and pathogenesis. By dot blot and immunofluorescence screenings, we found that MAb Psn2G6 specifically recognized Psn proteins in wing imaginal discs and brains of wild-type control W1118 larvae. MAb Psn2G6 also transgenically expressed a long form of wild-type Psn (Psn + 14 WT) proteins in wing imaginal discs of two independent transgenic lines. Transgenic expression of Psn + 14 WT proteins in psn(B3) larvae completely rescued the expression patterns of Psn proteins and the development of wing imaginal discs. In addition, neural hyperplasia observed in wing imaginal discs of psn(B3) larvae was also suppressed.
Cuc
Ho Thi Thu
HT
Ilsong Institute of Life Science, Hallym University , Dongan-gu, Anyang, Gyeonggi-do, Republic of Korea.
Seo
Jong Bok
JB
Choi
Jin Kyu
JK
Kim
Won Tae
WT
Park
Seok Jou
SJ
Lee
Dae Weon
DW
Kim
Yong Sun
YS
Fortini
Mark E
ME
Koh
Young Ho
YH
D000595:Amino Acid Sequence; D000818:Animals; D030801:Animals, Genetically Modified; D000911:Antibodies, Monoclonal / Q000276:immunology*; D004330:Drosophila; D005455:Fluorescent Antibody Technique; D008969:Molecular Sequence Data; D053763:Presenilins / Q000276:immunology*
D000911:Antibodies, Monoclonal; D053763:Presenilins