[Application of ITO method and discriminant functions in full sibling and half sibling identification].
OBJECTIVE
To investigate the application of ITO method and discriminant functions method in full sibling and half sibling identification.


METHODS
Five hundred pairs of full siblings (FS), 50 pairs of half siblings (HS) and 500 pairs of unrelated individuals (UR) were genotyped by PowerPlex 16 system. Full sibling index (FSI), half sibling index (HSI) and the FSI:HSI ratio were calculated with ITO method. Allelic matching of each pair of the three groups was compared. The locus numbers of no-allele sharing (x0), half-allele sharing (x1) and two-alleles sharing (x2) were calculated, respectively. The discriminant functions about full-siblings, half-siblings and unrelated individuals (UR) were established by SPSS 13.0 statistical software.


RESULTS
(1) Regard FSI > or = 19 or FSI < 1 as the standard of distinguishing full sibling from unrelated individual, the alternate correct percentage was 96.4%. Regard HSI > or = 19 or HSI < 1 as the standard of distinguishing half sibling from unrelated individual, the alternate correct percentage was 85.3%. Regard FSI:HSI > or = 1 or FSI:HSI < 1 as the standard of distinguishing full sibling from half sibling, the alternate correct percentage was 87.5%. (2) Four groups of discriminant functions were established. The alternate correct percentage of these discriminant functions were 84.4%-97.7%, with the highest one in full sibship-unrelated individual group.


CONCLUSIONS
Both ITO method and discriminant functions method are efficient in identification of full sibling or half sibling.
Lu
Hui-Ling
HL
Department of Forensic Biology, Zhongshan Medical College, Sun Yat-sen University, Guangzhou 510080, China. luhl@mail.sysu.edu.cn
Zhou
Ke-Wei
KW
Lü
De-Jian
DJ
Yao
Ya-Nan
YN
Zhang
Ya-Qing
YQ
D000483:Alleles; D016002:Discriminant Analysis; D053803:Forensic Genetics; D014644:Genetic Variation; D018392:Genomic Imprinting / Q000235:genetics*; D005838:Genotype; D006801:Humans; D010334:Paternity; D035781:Siblings; D020080:Tandem Repeat Sequences / Q000235:genetics*