title: Modelling and sensitivity analysis of the reactions involving receptor, G-protein and effector in vertebrate olfactory receptor neurons.
abstract: A biochemical model of the receptor, G-protein and effector (RGE) interactions during transduction in the cilia of vertebrate olfactory receptor neurons (ORNs) was developed and calibrated to experimental recordings of cAMP levels and the receptor current (RC). The model describes the steps from odorant binding to activation of the effector enzyme which catalyzes the conversion of ATP to cAMP, and shows how odorant stimulation is amplified and delayed by the RGE transduction cascade. A time-dependent sensitivity analysis was performed on the model. The model output-the cAMP production rate-is particularly sensitive to a few, dominant parameters. During odorant stimulation it depends mainly on the initial density of G-proteins and the catalytic constant for cAMP production.
authors.affiliation: School of Computer Science and Communication, Royal Institute of Technology, AlbaNova University Center, 106 91 Stockholm, Sweden. geirh@kth.se
authors.forename: Geir
authors.initials: G
authors.lastname: Halnes
authors.forename: Erik
authors.initials: E
authors.lastname: Ulfhielm
authors.forename: Emma
authors.initials: E
authors.lastname: Eklöf Ljunggren
authors.forename: Jeanette Hellgren
authors.initials: JH
authors.lastname: Kotaleski
authors.forename: Jean-Pierre
authors.initials: JP
authors.lastname: Rospars
mesh_terms: D000818:Animals; D001703:Biophysics / Q000379:methods; D002923:Cilia / Q000378:metabolism; D000242:Cyclic AMP / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D019204:GTP-Binding Proteins / Q000378:metabolism*; D007313:Insecta; D008959:Models, Neurological; D009812:Odorants; D018034:Olfactory Receptor Neurons / Q000502:physiology*; D010812:Physical Stimulation / Q000379:methods; D012680:Sensitivity and Specificity; D015398:Signal Transduction / Q000502:physiology*; D013268:Stimulation, Chemical
chemical_list: D000242:Cyclic AMP; D019204:GTP-Binding Proteins