[Experimental study on overactivation of lymphocytes and cytokines in immunopathogenesis of nasal polyp].
OBJECTIVE
To investigate the expression of CD4, CD69, CD34, RANTES, IL-5 and IL-8 in nasal polyp tissues, and study their roles in the formation of nasal polyp.


METHODS
The expression of CD4, CD69, CD34, RANTES, IL-5 and IL-8 were detected by immunohistochemical method and image analysis in 34 cases of nasal polyps and 30 cases of nasal concha mucosa (LNT).


RESULTS
The positive rate of glandular organ hyperplasia, formation of beaker cell, fiber hyperplasia, interstitial edema and infiltration of lymphocyte and eosinophilic granulocyte in nasal polyps were significantly higher than those in nasal concha mucosa (P<0.01). The cell density (piece/mm2) of CD4+, CD69+, IL-5, IL-8, RANTES in 34 nasal polyps was significantly higher than those in nasal concha mucosa (P<0.05). Marked positive correlations were found between expression of CD4, CD69 and RANTES, IL-5 and IL-8 (P<0.05, P<0.01 and P<0.05), expression of IL-5 and RANTES and infiltration level of eosinophilic granulocyte (P<0.05 and P<0.01), and expression of IL-8 and vaso formation on nasal polyps tissue (P<0.01).


CONCLUSIONS
T lymphocytes and correlated cytokines participate in the immunopathogenesis of nasal polyps; IL-5 and RANTES can prompt the infiltration, the aggregation and the activation of eosinophilic granulocytes; IL-8 can promote the vaso formation in nasal polyps.
Song
Zhongyi
Z
Institute for Preclinical Medicine, Shandong Academy of Medical Science, Jinan, 250062, China.
Zhang
Ling
L
Li
Jiandong
J
Li
Xingong
X
Li
Cuiling
C
D018946:Chemokine CCL5 / Q000378:metabolism; D005260:Female; D006098:Granulocytes / Q000276:immunology; D006801:Humans; D015848:Interleukin-5 / Q000378:metabolism; D016209:Interleukin-8 / Q000378:metabolism; D008213:Lymphocyte Activation; D018655:Lymphocyte Count; D008297:Male; D008875:Middle Aged; D009297:Nasal Mucosa / Q000276:immunology / Q000378:metabolism*; D009298:Nasal Polyps / Q000276:immunology / Q000378:metabolism*; D013601:T-Lymphocytes / Q000276:immunology*
C508606:CXCL8 protein, human; D018946:Chemokine CCL5; C508599:IL5 protein, human; D015848:Interleukin-5; D016209:Interleukin-8