Novel antimicrobial peptides isolated from skin secretions of the Mexican frog Hyla eximia.
In this work, we describe the original characterization of peptides and proteins present in the skin secretions of the Mexican amphibian Hyla eximia. To this purpose, a novel water/dark extraction method, as well as the classic electrical stimulation procedure, was applied in order to extract the skin secretion. Two novel antimicrobial peptides He-1 and He-2 were sequenced. In addition, a molecular mass fingerprint revealed more than one hundred different molecules. Eight peptides in homogeneous form were assayed against five species of bacteria. Thereafter, the peptide He-2 demonstrated high antiparasitic activity against ookinete forms of malaria parasites at low concentration.
Villa-Hernández
Oscar
O
Unidad de Proteómica, Instituto de Biotecnología-UNAM, Av. Universidad 2001, Col Chamilpa, CP 62210, Cuernavaca, Mor-México.
Hernández-Orihuela
Lorena
L
del Carmen Rodríguez
Maria
M
Zamudio-Zuñiga
Fernando
F
Castro-Franco
Ruben
R
Pando
Victoria
V
Batista
Cesar V F
CV
D000818:Animals; D023181:Antimicrobial Cationic Peptides / Q000302:isolation & purification* / Q000378:metabolism / Q000494:pharmacology; D001001:Anura; D001419:Bacteria; D012634:Bodily Secretions / Q000737:chemistry*; D049109:Cell Proliferation / Q000187:drug effects; D005591:Chemical Fractionation / Q000379:methods; D008800:Mexico; D008826:Microbial Sensitivity Tests; D010962:Plasmodium berghei; D020539:Sequence Analysis, Protein; D012867:Skin / Q000378:metabolism*
D023181:Antimicrobial Cationic Peptides