Lack of salicylic acid in Arabidopsis protects plants against moderate salt stress.
Previous studies showed that salicylic acid (SA)-deficient transgenic Arabidopsis expressing the salicylate hydroxylase gene NahG had a higher tolerance to moderate salt stress. SA may potentiate the stress response of germination and growth of Arabidopsis seedlings by inducing reactive oxygen species (ROS). However, the detailed mechanism for a better adaption of NahG plants to moderate salt stress is largely unknown. In the present study we found that a higher GSH/GSSG (glutathione/oxidized glutathione) ratio and ASA/DHA (ascorbic acid/dehydroascorbate) ratio in NahG plants during the stress may be the key reason for their stress-tolerance advantage. NahG plants actually could not produce more active antioxidant enzymes than the wild-type ones under natural conditions, but maintain higher activities of glutathione reductase (GR) and dehydroascorbate reductase (DHAR) during the stress. Hereby, the reduced glutathione and reduced ascorbic acid contents are higher in NahG plants under salt stress. However, NahG plants do not adapt better under severe salt stress. All antioxidant enzyme activities, GSH/GSSG ratio and ASA/DHA ratio declined substantively at 400 mM NaCl stress in both NahG and wild-type seedlings.
Cao
Yang
Y
Ministry of Education Key Laboratory for Bio-Resource and Eco-Environment, College of Life Science, Sichuan University, Chengdu 610064, China.
Zhang
Zhong-Wei
ZW
Xue
Li-Wei
LW
Du
Jun-Bo
JB
Shang
Jing
J
Xu
Fei
F
Yuan
Shu
S
Lin
Hong-Hui
HH
D017360:Arabidopsis / Q000187:drug effects / Q000235:genetics / Q000254:growth & development / Q000502:physiology*; D029681:Arabidopsis Proteins / Q000378:metabolism; D001205:Ascorbic Acid / Q000378:metabolism; D005978:Glutathione / Q000378:metabolism; D019803:Glutathione Disulfide / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000235:genetics / Q000378:metabolism; D005980:Glutathione Reductase / Q000378:metabolism; D030821:Plants, Genetically Modified / Q000187:drug effects / Q000502:physiology*; D016133:Polymerase Chain Reaction; D018749:RNA, Plant / Q000235:genetics / Q000302:isolation & purification; D020156:Salicylic Acid / Q000378:metabolism*; D012492:Salts / Q000009:adverse effects; D014158:Transcription, Genetic
D029681:Arabidopsis Proteins; D018749:RNA, Plant; D012492:Salts; D005979:Glutathione Peroxidase; D005980:Glutathione Reductase; D005978:Glutathione; D020156:Salicylic Acid; D001205:Ascorbic Acid; D019803:Glutathione Disulfide