The effects of maternal deprivation on the hippocampal structure in adult rats.
OBJECTIVE
To examine the ultrastructural effects of maternal deprivation during developmental periods of limbic-hypothalamo-pituitary-adrenal system on hippocampal dendritic structures in adult rats.


METHODS
The experiments were carried out with male and female Wistar rats in our department. The rats were mated and, after birth, the pups were divided into four groups. The first group (control group) pups remained undisturbed with their dam until postweaning day 22. Maternal deprived groups were separated from their dams for 24 hours at postnatal day 4, 9 and 18. The subjects were provided with food and water ad libitum until 3-months-of-age. At the third month, the rats were transcardially perfused, samples were taken from CA1 and CA3 regions of the hippocampus. Tissues were prepared for electron microscopy.


RESULTS
When the data were analyzed, there were no differences between male and female rats in both ultrastructure and semiquantitative analysis of axodendritic synapses. The ultrastructure of Group 1 was seen as normal while in the second Group some neurons nuclear envelope made deep invagination into the nucleus. Additionally, axodendritic synapses were found normal. In Group 3, micrographs and axodendritic synapses were showed normal structure. However, in Group 4 in some neurons invaginations were seen similar to Group 2. Axodendritic synapses were found to be normal.


CONCLUSIONS
These experiments establish that MD in rats produces slight ultrastructural changes and decreases the number of synapses in CA1 and CA3 subregions of the hippocampus.
Karakaş
Pinar
P
Department of Anatomy, Cukurova University, Faculty of Medicine, 01330 Adana, Turkey.
Bozkir
Memduha Gülhal
MG
Dere
Fahri
F
Melik
Enver
E
Melik
Emine Babar
EB
Kaya
Mehmet
M
Polat
Sait
S
D000367:Age Factors; D000704:Analysis of Variance; D000818:Animals; D000831:Animals, Newborn; D003712:Dendrites / Q000648:ultrastructure; D005260:Female; D006624:Hippocampus / Q000166:cytology* / Q000254:growth & development; D008297:Male; D008426:Maternal Deprivation; D046529:Microscopy, Electron, Transmission / Q000379:methods; D009474:Neurons / Q000166:cytology*; D051381:Rats; D017208:Rats, Wistar