A genomic approach to yeast chronological aging.
Yeast is a useful model organism to study the genetic and biochemical mechanisms of aging. Genomic studies of aging in yeast have been limited, however, by traditional methodologies that require a large investment of labor and resources. In this chapter, we describe a newly-developed method for quantitatively measuring the chronological life span of each strain contained in the yeast ORF deletion collection. Our approach involves determining population survival by monitoring outgrowth kinetics using a Bioscreen C MBR shaker/incubator/plate reader. This method has accuracy comparable to traditional assays, while allowing for higher throughput and decreased variability in measurement.
Burtner
Christopher R
CR
Department of Pathology, University of Washington, Seattle, WA 98195-7470, USA.
Murakami
Christopher J
CJ
Kaeberlein
Matt
M
D003470:Culture Media / Q000737:chemistry; D023281:Genomics / Q000295:instrumentation / Q000379:methods* / Q000706:statistics & numerical data; D008957:Models, Genetic; D016366:Open Reading Frames; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000235:genetics* / Q000254:growth & development*; D017384:Sequence Deletion; D013997:Time Factors
D003470:Culture Media