Evaluation of Fusarium oxysporum cellulolytic system for an efficient hydrolysis of hydrothermally treated wheat straw.
The crude multienzyme extract produced by Fusarium oxysporum cultivated under submerged conditions in 20 L bioreactor using brewers spent grain and corn cobs in a ratio 2:1 as the carbon source was evaluated with regard to an efficient saccharification of hydrothermally treated wheat straw. Several factors concerning the obtained hydrolysis yield and reaction rate were investigated. The takeout of product sugars (in situ) was effective at reducing end-product inhibition and lead to a bioconversion about 80% of the theoretical. A kinetic model incorporating dynamic adsorption, enzymatic hydrolysis, and product inhibition was developed. The model predicted very satisfactorily the experimental data.
Xiros
Charilaos
C
Biotechnology Laboratory, School of Chemical Engineering, National Technical University of Athens, 9 Iroon Polytechniou Street, Zografou Campus, 15700 Athens, Greece.
Katapodis
Petros
P
Christakopoulos
Paul
P
D018533:Biomass; D019149:Bioreactors; D002482:Cellulose / Q000378:metabolism*; D005670:Fusarium / Q000187:drug effects* / Q000378:metabolism*; D005947:Glucose / Q000302:isolation & purification / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration / Q000187:drug effects; D006868:Hydrolysis / Q000187:drug effects; D007700:Kinetics; D008954:Models, Biological; D012710:Serum Albumin, Bovine / Q000378:metabolism; D013501:Surface-Active Agents / Q000494:pharmacology; D013696:Temperature; D014908:Triticum / Q000187:drug effects* / Q000378:metabolism*; D014867:Water / Q000494:pharmacology*
D013501:Surface-Active Agents; D014867:Water; D012710:Serum Albumin, Bovine; D002482:Cellulose; D005947:Glucose