Regulatory role of nitric oxide in neutrophil apoptosis.
Apoptosis of blood neutrophils from healthy donors was studied under conditions of cell culturing with different concentrations of H(2)O(2), selective NO synthase inhibitor, and inductor of NO synthesis (L-arginine). In vitro incubation of neutrophilic leukocytes with 5 mM H(2)O(2) led to activation of the apoptotic program in neutrophils, which was seen from increased content of Bax protein in the cells and increased number of apoptotic cells in the culture. Increased content of annexin-positive cells after incubation of neutrophil culture with NO synthase inhibitor suggests involvement of NO in the regulation of neutrophil apoptosis under conditions of oxidative stress, while L-arginine prevented H(2)O(2)-induced programmed cell death.
Stepovaya
Ye A
YA
Department of Fundamentals of Clinical Medicine, Laboratory of Molecular Medicine, Siberian State Medical University, Tomsk, Russia.
Zhavoronok
T V
TV
Starikov
Yu V
YV
Bychkov
V A
VA
Chasovskih
N Yu
NY
Starikova
Ye G
YG
Petina
G V
GV
Novitsky
V V
VV
Ryazantseva
N V
NV
D000293:Adolescent; D000328:Adult; D017302:Annexins / Q000378:metabolism; D017209:Apoptosis / Q000187:drug effects; D001120:Arginine / Q000494:pharmacology; D015153:Blotting, Western; D002478:Cells, Cultured; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005260:Female; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology; D008297:Male; D019331:NG-Nitroarginine Methyl Ester / Q000494:pharmacology; D009504:Neutrophils / Q000187:drug effects / Q000378:metabolism*; D009569:Nitric Oxide / Q000378:metabolism / Q000502:physiology*; D019001:Nitric Oxide Synthase / Q000037:antagonists & inhibitors; D016877:Oxidants / Q000494:pharmacology; D018384:Oxidative Stress / Q000502:physiology; D017382:Reactive Oxygen Species / Q000378:metabolism; D055815:Young Adult; D051028:bcl-2-Associated X Protein / Q000378:metabolism
D017302:Annexins; D004791:Enzyme Inhibitors; D016877:Oxidants; D017382:Reactive Oxygen Species; D051028:bcl-2-Associated X Protein; D009569:Nitric Oxide; D001120:Arginine; D006861:Hydrogen Peroxide; D019001:Nitric Oxide Synthase; D019331:NG-Nitroarginine Methyl Ester