title: Cellular dichotomy between anchorage-independent growth responses to bFGF and TPA reflects molecular switch in commitment to carcinogenesis.
abstract: We have investigated gene expression patterns underlying reversible and irreversible anchorage-independent growth (AIG) phenotypes to identify more sensitive markers of cell transformation for studies directed at interrogating carcinogenesis responses. In JB6 mouse epidermal cells, basic fibroblast growth factor (bFGF) induces an unusually efficient and reversible AIG response, relative to 12-O-tetradecanoyl phorbol-13-acetate (TPA)-induced AIG which is irreversible. The reversible and irreversible AIG phenotypes are characterized by largely nonoverlapping global gene expression profiles. However, a subset of differentially expressed genes were identified as common to reversible and irreversible AIG phenotypes, including genes regulated in a reciprocal fashion. Hepatic leukemia factor (HLF) and D-site albumin promoter-binding protein (DBP) were increased in both bFGF and TPA soft agar colonies and selected for functional validation. Ectopic expression of human HLF and DBP in JB6 cells resulted in a marked increase in TPA- and bFGF-regulated AIG responses. HLF and DBP expression were increased in soft agar colonies arising from JB6 cells exposed to gamma radiation and in a human basal cell carcinoma tumor tissue, relative to paired nontumor tissue. Subsequent biological network analysis suggests that many of the differentially expressed genes that are common to bFGF- and TPA-dependent AIG are regulated by c-Myc, SP-1, and HNF-4 transcription factors. Collectively, we have identified a potential molecular switch that mediates the transition from reversible to irreversible AIG.
authors.affiliation: Computational Biology and Bioinformatics Groups, Pacific Northwest National Laboratory, Richland, Washington 99354, USA.
authors.forename: Katrina M
authors.initials: KM
authors.lastname: Waters
authors.forename: Ruimin
authors.initials: R
authors.lastname: Tan
authors.forename: Lee K
authors.initials: LK
authors.lastname: Opresko
authors.forename: Ryan D
authors.initials: RD
authors.lastname: Quesenberry
authors.forename: Somnath
authors.initials: S
authors.lastname: Bandyopadhyay
authors.forename: William B
authors.initials: WB
authors.lastname: Chrisler
authors.forename: Thomas J
authors.initials: TJ
authors.lastname: Weber
mesh_terms: D000818:Animals; D001483:Base Sequence; D050976:Basic-Leucine Zipper Transcription Factors / Q000378:metabolism; D002448:Cell Adhesion / Q000187:drug effects*; D002455:Cell Division / Q000187:drug effects*; D045744:Cell Line, Tumor; D002471:Cell Transformation, Neoplastic; D017931:DNA Primers; D004268:DNA-Binding Proteins / Q000378:metabolism; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology*; D006801:Humans; D051379:Mice; D020411:Oligonucleotide Array Sequence Analysis; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*; D014157:Transcription Factors / Q000378:metabolism; D014158:Transcription, Genetic
chemical_list: D050976:Basic-Leucine Zipper Transcription Factors; C063898:DBP protein, human; D017931:DNA Primers; D004268:DNA-Binding Proteins; C490671:HLF protein, human; D014157:Transcription Factors; D016222:Fibroblast Growth Factor 2; D013755:Tetradecanoylphorbol Acetate