title: Direct pulp capping with a self-etching adhesive system: histopathologic evaluation in dogs' teeth.
abstract: OBJECTIVE
  This study evaluated histopathologically the response of pulp and periradicular tissues after pulp capping with an all-in-one self-etching adhesive system in dogs' teeth.
  
  
  METHODS
  Forty teeth of 4 dogs were assigned to 3 groups according to the pulp capping material: G1 (n = 20): self-etching adhesive system; G2 (n = 10): Ca(OH)(2); G3 (n = 10): zinc oxide-eugenol. The animals were killed 7 and 70 days after pulp capping. The pieces containing the pulp-capped teeth were removed and processed for histologic analysis.
  
  
  RESULTS
  At 7 days, no dentin bridge formation was observed; G1 and G3 exhibited inflammatory pulpal alterations, whereas G2 presented only mild inflammatory infiltrate in the pulp tissue adjacent to the capping material, the remainder being intact. At 70 days, no specimen in G1 or G3 presented dentin bridge formation. The remaining pulp tissue exhibited severe inflammatory alterations and areas of necrosis. In G2, all specimens showed dentin bridge formation and absence of inflammation and mineralized tissue resorption. No bacteria were identified using Brown and Brenn staining techniques in all 3 groups at any observation period.
  
  
  CONCLUSIONS
  According to the conditions of this study, direct pulp capping with the self-etching adhesive system did not allow pulp tissue repair and failed histopathologically in 100% of the cases.
authors.affiliation: Department of Pediatric Clinics, Preventive and Community Dentistry, School of Dentistry of Ribeirão Preto, University of São Paulo, Ribeirão Preto, Brazil. lea@forp.usp.br
authors.forename: Lea A B
authors.initials: LA
authors.lastname: da Silva
authors.forename: Aldevina C
authors.initials: AC
authors.lastname: de Freitas
authors.forename: Fabrício K
authors.initials: FK
authors.lastname: de Carvalho
authors.forename: Alexandra M
authors.initials: AM
authors.lastname: de Queiroz
authors.forename: Paulo
authors.initials: P
authors.lastname: Nelson-Filho
authors.forename: Sizenando T
authors.initials: ST
authors.lastname: Porto-Neto
mesh_terms: D000818:Animals; D002126:Calcium Hydroxide / Q000627:therapeutic use; D003094:Collagen; D003188:Composite Resins; D003738:Dental Cements / Q000627:therapeutic use; D003764:Dental Materials / Q000627:therapeutic use; D003782:Dental Pulp / Q000473:pathology*; D003785:Dental Pulp Capping / Q000379:methods*; D003789:Dental Pulp Exposure / Q000628:therapy; D003790:Dental Pulp Necrosis / Q000473:pathology; D003793:Dental Restoration, Permanent; D003804:Dentin / Q000473:pathology; D003809:Dentin, Secondary / Q000473:pathology; D017220:Dentin-Bonding Agents / Q000627:therapeutic use*; D004285:Dogs; D005260:Female; D005347:Fibroblasts / Q000473:pathology; D008297:Male; D009804:Odontoblasts / Q000473:pathology; D010486:Periapical Tissue / Q000473:pathology*; D011671:Pulpitis / Q000473:pathology; D019279:Resin Cements / Q000627:therapeutic use; D015035:Zinc Oxide-Eugenol Cement / Q000627:therapeutic use
chemical_list: D003188:Composite Resins; D003738:Dental Cements; D003764:Dental Materials; D017220:Dentin-Bonding Agents; C444701:Filtek Z250; C408584:Prompt L-Pop; D019279:Resin Cements; D015035:Zinc Oxide-Eugenol Cement; D003094:Collagen; D002126:Calcium Hydroxide