Agouti C57BL/6N embryonic stem cells for mouse genetic resources.
We report the characterization of a highly germline competent C57BL/6N mouse embryonic stem cell line, JM8. To simplify breeding schemes, the dominant agouti coat color gene was restored in JM8 cells by targeted repair of the C57BL/6 nonagouti mutation. These cells provide a robust foundation for large-scale mouse knockout programs that aim to provide a public resource of targeted mutations in the C57BL/6 genetic background.
Pettitt
Stephen J
SJ
Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Cambridge, UK.
Liang
Qi
Q
Rairdan
Xin Y
XY
Moran
Jennifer L
JL
Prosser
Haydn M
HM
Beier
David R
DR
Lloyd
Kent C
KC
Bradley
Allan
A
Skarnes
William C
WC
D054366:Agouti Signaling Protein / Q000235:genetics*; D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D003433:Crosses, Genetic; D017931:DNA Primers / Q000235:genetics; D053595:Embryonic Stem Cells / Q000166:cytology* / Q000378:metabolism*; D005260:Female; D018390:Gene Targeting; D005821:Genetic Techniques; D018095:Germ-Line Mutation; D006200:Hair Color / Q000235:genetics; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL / Q000145:classification / Q000235:genetics*; D018345:Mice, Knockout; D008817:Mice, Mutant Strains; D008822:Mice, Transgenic; D020641:Polymorphism, Single Nucleotide; D011247:Pregnancy; D018183:Transplantation Chimera / Q000235:genetics
D054366:Agouti Signaling Protein; D017931:DNA Primers; C515183:a protein, mouse