Immobilization of RGD peptide on HA coating through a chemical bonding approach.
In this work, Arg-Gly-Asp (RGD) sequence containing peptide was immobilized on hydroxyapatite (HA) coatings through a chemical bonding approach in two steps, surface modification with 3-aminopropyltriethoxysilane (APTES) and RGD immobilization. The results indicate that RGD has been successfully immobilized on HA coatings. Comparing with physical adsorption coatings, the chemically bonded RGD on the coatings shows much better anti-wash-out ability. Since RGD is able to recognize cell-membrane integrins on biointerfaces, the present method will be an effective way to favor interaction of cells with HA coatings.
Yang
Chunli
C
Department of Materials Science and Engineering, State Key Laboratory of Silicon Materials, Zhejiang University, Hangzhou, Zhejiang 310027, People's Republic of China.
Cheng
Kui
K
Weng
Wenjian
W
Yang
Chunyu
C
D000327:Adsorption; D001672:Biocompatible Materials / Q000737:chemistry*; D002448:Cell Adhesion / Q000187:drug effects; D002462:Cell Membrane / Q000378:metabolism; D017886:Durapatite / Q000737:chemistry*; D005109:Extracellular Matrix / Q000378:metabolism; D006801:Humans; D016023:Integrins / Q000378:metabolism; D008024:Ligands; D009842:Oligopeptides / Q000737:chemistry*; D010455:Peptides / Q000737:chemistry; D011437:Propylamines; D011485:Protein Binding; D012821:Silanes / Q000737:chemistry; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods; D013499:Surface Properties; D014465:Ultrasonics; D014867:Water / Q000737:chemistry
D001672:Biocompatible Materials; D016023:Integrins; D008024:Ligands; D009842:Oligopeptides; D010455:Peptides; D011437:Propylamines; D012821:Silanes; D014867:Water; C047981:arginyl-glycyl-aspartic acid; D017886:Durapatite; C477625:amino-propyl-triethoxysilane