Formation of DNA toroids inside confined droplets adsorbed on mica surfaces.
We report observations of in vitro DNA compaction into toroids in the absence of any condensing agent. The DNA toroid formation is induced by geometry confinement from microdroplets on mica surfaces. With AFM imaging we show that the confined DNA molecules may take the form of random coils or semiordered folded loops with large microdroplets, while they readily take the form of compact and ordered toroids when the microdroplet sizes are small enough. To better understand these phenomena, we carried out coarse-grained Brownian dynamics simulation, obtaining results that were in good agreement with the experimental observations. The simulation reveals that the toroid formation is sensitive to not only the microdroplet size, but also the DNA stiffness.
Hou
Xi-Miao
XM
Laboratory of Soft Matter Physics, Beijing National Laboratory for Condensed Matter Physics, Institute of Physics, Chinese Academy of Sciences, Beijing 100190, China.
Li
Wei
W
Dou
Shuo-Xing
SX
Zhang
Ling-Yun
LY
Xie
Ping
P
Wang
Wei-Chi
WC
Wang
Peng-Ye
PY
D000538:Aluminum Silicates / Q000737:chemistry*; D003198:Computer Simulation; D004247:DNA / Q000737:chemistry* / Q000648:ultrastructure*; D008956:Models, Chemical; D008958:Models, Molecular; D009690:Nucleic Acid Conformation; D012996:Solutions; D013499:Surface Properties
D000538:Aluminum Silicates; D012996:Solutions; D004247:DNA; C011934:mica