title: The expression analysis of periostin in human breast cancer.
abstract: BACKGROUND
  To investigate the expression of periostin in breast cancer and its clinical relevance.
  
  
  METHODS
  Periostin mRNA expression in breast tissues was measured by RT-PCR. Periostin protein in breast tissues was evaluated by Western blot. Immunohistochemistry method (Envision method) was carried out to detect the expression of periostin in breast cancer tissues of different clinical stages and metastasized lymph nodes, as well as benign breast disease.
  
  
  RESULTS
  The expression of periostin in cancer tissue was increased at both mRNA level (P < 0.05) and protein level (P < 0.05) compared with benign and normal breast tissues. The periostin protein expression level increased with breast cancer clinical stages as shown by IHC.
  
  
  CONCLUSIONS
  Periostin was overexpressed in breast cancer tissues as opposed to normal breast, which implies its role in the pathogenesis and development of this disorder.
authors.affiliation: Department of General Surgery, Affiliated Zhong-Da Hospital, Southeast University, Nanjing, China. zhangyanan79@yahoo.com.cn
authors.forename: Yanan
authors.initials: Y
authors.lastname: Zhang
authors.forename: Guangyuan
authors.initials: G
authors.lastname: Zhang
authors.forename: Junsheng
authors.initials: J
authors.lastname: Li
authors.forename: Qingsong
authors.initials: Q
authors.lastname: Tao
authors.forename: Wenhao
authors.initials: W
authors.lastname: Tang
mesh_terms: D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000378:metabolism*; D001943:Breast Neoplasms / Q000378:metabolism*; D018270:Carcinoma, Ductal, Breast / Q000378:metabolism*; D015815:Cell Adhesion Molecules / Q000378:metabolism*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007150:Immunohistochemistry; D008875:Middle Aged; D012333:RNA, Messenger / Q000378:metabolism; D015854:Up-Regulation
chemical_list: D014408:Biomarkers, Tumor; D015815:Cell Adhesion Molecules; C512835:POSTN protein, human; D012333:RNA, Messenger