Identification of a novel relish homolog in Chinese shrimp Fenneropenaeus chinensis and its function in regulating the transcription of antimicrobial peptides.
Penaeid shrimp, as an invertebrate, relies on the innate immunity to oppose the microbial invaders. Antimicrobial peptides (AMP) are an integral component of the innate immune system in most organisms and function as an early first line of defense against pathogens, but the knowledge about the pathways to regulate the shrimp AMP gene expression is still absent up to date. In the current study, a Relish homolog (FcRelish) was cloned from Chinese shrimp Fenneropenaeus chinensis. The full length cDNA of FcRelish consists of 2157 bp, including 1512 bp open reading frame, encoding 504 amino acids. The predicted molecular weight of FcRelish is 57 kDa, and the theoretical PI is 7.00. Spatial expression profiles showed that FcRelish had the highest expression levels in the hemocytes and lymphoid organ. Both Vibrio anguillarium and Micrococcus lysodeikticus stimulation to shrimp can affect the transcription profile of FcRelish. Silencing of FcRelish through DsRNA interference can greatly change the transcription profile of AMP. Therefore, we suggest that FcRelish identified in the present study is closely related to the transcription of AMP, and then we inferred that Imd pathway might exist in shrimp.
Li
Fuhua
F
Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China. fhli@ms.qdio.ac.cn
Yan
Hui
H
Wang
Dongdong
D
Priya
T A Jose
TA
Li
Shihao
S
Wang
Bing
B
Zhang
Jiquan
J
Xiang
Jianhai
J
D000595:Amino Acid Sequence; D000818:Animals; D023181:Antimicrobial Cationic Peptides / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D006434:Hemocytes / Q000378:metabolism; D054884:Host-Pathogen Interactions; D008837:Micrococcus / Q000502:physiology; D008969:Molecular Sequence Data; D033561:Penaeidae / Q000235:genetics* / Q000382:microbiology; D010802:Phylogeny; D034622:RNA Interference; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D014157:Transcription Factors / Q000145:classification / Q000235:genetics*; D014158:Transcription, Genetic / Q000235:genetics*; D014733:Vibrio / Q000502:physiology
D023181:Antimicrobial Cationic Peptides; D018076:DNA, Complementary; D014157:Transcription Factors