Immunoaffinity column clean-up techniques in food analysis: A review.
This review provides a critical assessment of the applications of immunoaffinity columns for sample clean-up in the field of food safety. The performance of immunoaffinity columns are compared in terms of specificity, binding capacity and recovery, and commercial disposable columns are contrasted with home-made columns. Areas covered include multiple-use of columns, multiple-analyte columns, use with automated systems and validation of IAC methods. Publications illustrating the many varied applications of IAC for sample clean-up in the areas of mycotoxins, veterinary drug residues, pesticide residues, environmental contaminants and vitamins have been compiled, comparing extraction methods, achievable recovery, and illustrating the variety of end-detection methods that have been employed.
Senyuva
Hamide Z
HZ
Scientific and Technological Research Council of Turkey, Ankara, Turkey. hamide.senyuva@tubitak.gov.tr
Gilbert
John
J
D000906:Antibodies / Q000737:chemistry; D002846:Chromatography, Affinity / Q000379:methods*; D005504:Food Analysis / Q000379:methods*; D005506:Food Contamination / Q000032:analysis*; D007163:Immunosorbent Techniques
D000906:Antibodies