title: Identification of novel functional differences in monocyte subsets using proteomic and transcriptomic methods.
abstract: Human blood monocytes can be broadly divided into two distinct subsets: CD14+CD16- and CD14+/lowCD16+ subsets. Perturbation in their proportions in the blood has been observed in several disease conditions. Although numerous phenotypic and functional differences between the two subsets have already been described, the roles contributed by each subset during homeostasis or disease conditions are still largely unclear. To uncover novel differences to aid in elucidating their functions, we perform a global analysis of the two subsets utilizing both proteomics and transcriptomics approaches. From the proteomics and transcriptomics data, the expression of 613 genes by the two subsets is detected at both the protein and mRNA levels. These 613 genes are assessed for up-regulation in each subset at the protein and mRNA levels using a cutoff fold change of > or =|1.5| between subsets. Proteins and mRNAs up-regulated in each subset are then mapped in silico into biological functions. This mapping reveals copious functional differences between the subsets, many of which are seen at both protein and mRNA levels. For instance, expression of genes involved in F(CY) receptor-mediated phagocytosis are up-regulated in the CD14+/lowCD16+ subset, while those involved in antimicrobial function are up-regulated in the CD14+CD16- subset. We uncover novel functional differences between the monocyte subsets from differences in gene expression at the protein and mRNA levels. These functional differences would provide new insights into the different roles of the two monocyte subsets in regulating innate and adaptive immune responses.
authors.affiliation: Singapore Immunology Network, Agency for Science, Technology and Research, Biopolis, Singapore.
authors.forename: Changqing
authors.initials: C
authors.lastname: Zhao
authors.forename: Huoming
authors.initials: H
authors.lastname: Zhang
authors.forename: Wing-Cheong
authors.initials: WC
authors.lastname: Wong
authors.forename: Xiaohui
authors.initials: X
authors.lastname: Sem
authors.forename: Hao
authors.initials: H
authors.lastname: Han
authors.forename: Siew-Min
authors.initials: SM
authors.lastname: Ong
authors.forename: Yann-Chong
authors.initials: YC
authors.lastname: Tan
authors.forename: Wei-Hseun
authors.initials: WH
authors.lastname: Yeap
authors.forename: Chee-Sian
authors.initials: CS
authors.lastname: Gan
authors.forename: Kok-Quan
authors.initials: KQ
authors.lastname: Ng
authors.forename: Mickey Boon-Chai
authors.initials: MB
authors.lastname: Koh
authors.forename: Philippe
authors.initials: P
authors.lastname: Kourilsky
authors.forename: Siu-Kwan
authors.initials: SK
authors.lastname: Sze
authors.forename: Siew-Cheng
authors.initials: SC
authors.lastname: Wong
mesh_terms: D001770:Blood Bactericidal Activity; D001798:Blood Proteins / Q000378:metabolism*; D016000:Cluster Analysis; D003198:Computer Simulation; D003627:Data Interpretation, Statistical; D058851:GPI-Linked Proteins; D020869:Gene Expression Profiling / Q000379:methods; D006801:Humans; D007553:Isotope Labeling; D018950:Lipopolysaccharide Receptors / Q000097:blood; D009000:Monocytes / Q000145:classification / Q000378:metabolism / Q000502:physiology*; D010587:Phagocytosis; D040901:Proteomics / Q000379:methods*; D017382:Reactive Oxygen Species / Q000378:metabolism; D017452:Receptors, IgG / Q000097:blood; D015203:Reproducibility of Results
chemical_list: D001798:Blood Proteins; C428200:FCGR3B protein, human; D058851:GPI-Linked Proteins; D018950:Lipopolysaccharide Receptors; D017382:Reactive Oxygen Species; D017452:Receptors, IgG