title: RASSF2A promoter methylation in hepatitis B virus-related hepatocellular carcinogenesis and its correlation with elevated serum alpha-fetoprotein level.
abstract: Loss of the RASSF2A expression induced by methylation-mediated silencing has been reported in several human cancers, but the methylation status of RASSF2A in hepatocellular carcinoma (HCC) is rarely studied so far. In this study, we investigated the RASSF2A expression and its methylation status in a cohort of 45 hepatitis B virus-associated HCC tissues and their adjacent non-carcinoma tissues by using RT-PCR and MS-PCR. Promoter methylation of RASSF2A was found in 31 (68.9%) out of 45 HCC tissues and 12 (40%) out of 30 adjacent normal tissues, respectively (P<0.05). The methylation status of PASSF2A was closely associated with the loss of RASSF2A expression and elevated serum alpha-fetoprotein level, but not significantly with clinical stage, hepatic fibrosis and K-ras mutation. It was concluded that aberrant methylation of the RASSF2A gene with the subsequent loss of RASSF2A expression plays an important role in the pathogenesis of HCC.
authors.affiliation: Cancer Center, Huazhong University of Science and Technology, Wuhan, 430022, China. hustren2007@163.com
authors.forename: Jinghua
authors.initials: J
authors.lastname: Ren
authors.forename: Wenshan
authors.initials: W
authors.lastname: He
authors.forename: Ruiguang
authors.initials: R
authors.lastname: Zhang
authors.forename: Zhenyu
authors.initials: Z
authors.lastname: Li
authors.forename: Wenmiao
authors.initials: W
authors.lastname: Cao
authors.forename: Jie
authors.initials: J
authors.lastname: Yao
authors.forename: Fang
authors.initials: F
authors.lastname: Zhu
authors.forename: Tao
authors.initials: T
authors.lastname: Zhang
authors.forename: Gang
authors.initials: G
authors.lastname: Wu
mesh_terms: D000328:Adult; D000368:Aged; D006528:Carcinoma, Hepatocellular / Q000097:blood / Q000235:genetics* / Q000821:virology; D019175:DNA Methylation / Q000235:genetics*; D005260:Female; D006509:Hepatitis B / Q000150:complications* / Q000821:virology; D006801:Humans; D008113:Liver Neoplasms / Q000097:blood / Q000235:genetics* / Q000821:virology; D008297:Male; D008875:Middle Aged; D011401:Promoter Regions, Genetic / Q000235:genetics; D025521:Tumor Suppressor Proteins / Q000235:genetics*; D055815:Young Adult; D000509:alpha-Fetoproteins / Q000378:metabolism*
chemical_list: C425300:RASSF2 protein, human; D025521:Tumor Suppressor Proteins; D000509:alpha-Fetoproteins