title: High-throughput genotyping of KIR2DL2/L3, KIR3DL1/S1, and their HLA class I ligands using real-time PCR.
abstract: Killer immunoglobulin-like receptors (KIRs) expressed on natural killer cells are critical components of innate immunity. Interactions between KIRs and their human leukocyte antigen (HLA) ligands have been shown to influence autoimmune and infectious disease course in defined populations. However, the low throughput and high cost of current methods impede confirmation of the universality of these findings. To support large epidemiology surveys, we developed a high-throughput real-time polymerase chain reaction-based assay to identify carriers of KIR3DL1, KIR3DS1, KIR2DL2, and KIR2DL3 and their HLA ligands. The platform performed with 100% sensitivity and specificity in detection of carrier and non-carrier on reference samples. The application of this platform will further clarify the nature and impact of the KIR-HLA epistatic interaction on disease course in large global population-based studies.
authors.affiliation: Division of Retrovirology, US Military HIV Research Program/Henry M. Jackson Foundation, Rockville, MD 20850, USA. rkoehler@hivresearch.org
authors.forename: R N
authors.initials: RN
authors.lastname: Koehler
authors.forename: A M
authors.initials: AM
authors.lastname: Walsh
authors.forename: N
authors.initials: N
authors.lastname: Moqueet
authors.forename: J R
authors.initials: JR
authors.lastname: Currier
authors.forename: M A
authors.initials: MA
authors.lastname: Eller
authors.forename: L A
authors.initials: LA
authors.lastname: Eller
authors.forename: F
authors.initials: F
authors.lastname: Wabwire-Mangen
authors.forename: N L
authors.initials: NL
authors.lastname: Michael
authors.forename: M L
authors.initials: ML
authors.lastname: Robb
authors.forename: F E
authors.initials: FE
authors.lastname: McCutchan
authors.forename: G H
authors.initials: GH
authors.lastname: Kijak
mesh_terms: D000483:Alleles; D005838:Genotype; D015395:Histocompatibility Antigens Class I / Q000235:genetics*; D006801:Humans; D008024:Ligands; D016133:Polymerase Chain Reaction / Q000379:methods*; D054343:Receptors, KIR2DL2 / Q000235:genetics*; D054344:Receptors, KIR2DL3 / Q000235:genetics*; D054341:Receptors, KIR3DL1 / Q000235:genetics*; D054349:Receptors, KIR3DS1 / Q000235:genetics*; D012680:Sensitivity and Specificity
chemical_list: D015395:Histocompatibility Antigens Class I; C515077:KIR2DL2 protein, human; C515078:KIR2DL3 protein, human; C515083:KIR3DL1 protein, human; D008024:Ligands; D054343:Receptors, KIR2DL2; D054344:Receptors, KIR2DL3; D054341:Receptors, KIR3DL1; D054349:Receptors, KIR3DS1