title: Crystallographic cryoenzymology and the legacy of Tony Fink.
abstract: During the past thirty years significant contributions to our understanding of the structural origins of the catalytic power of enzymes have come from solution and crystallographic studies of enzyme-substrate and enzyme-intermediate complexes trapped at subzero temperatures, a field that was pioneered in large part by Anthony L. Fink and Pierre Douzou. Here I review, from a personal perspective, the history of crystallographic cryoenzymology, with an emphasis on the contributions of Tony Fink. The story has a moral: if you choose your collaborators based not only on their scientific prowess but also on their human qualities, the resulting friendships will enrich your life.
authors.affiliation: Rosenstiel Basic Medical Sciences Research Center, Brandeis University, 415 South Street Waltham, MA 02454-9110, USA. petsko@brandeis.edu
authors.forename: Gregory A
authors.initials: GA
authors.lastname: Petsko
mesh_terms: D001671:Biochemistry / Q000266:history / Q000379:methods*; D002384:Catalysis; D003460:Crystallization; D018360:Crystallography, X-Ray / Q000379:methods*; D004798:Enzymes; D005615:Freezing; D005962:Glucosylceramidase / Q000737:chemistry; D049673:History, 20th Century; D006801:Humans; D007700:Kinetics; D013379:Substrate Specificity
chemical_list: D004798:Enzymes; D005962:Glucosylceramidase