Assay conditions and validation of a new UV spectrophotometric method using microplates for the determination of polyphenol content.
A new spectrophotometric assay for the quantification of polyphenolic content has been validated. It is based on Prussian Blue method and adapted to microplate spectrophotometry. Prussian Blue reaction is critically dependent on reaction time, but microplate spectrophotometry permits exact measures of many samples at the same time. This new method is precise, reproducible, repeatable and exact.
Pueyo
Iñigo Uriarte
IU
Department of Pharmacy and Pharmaceutical Technology, School of Pharmacy, University of Navarra, Pamplona (Navarra), Spain. iuripue@yahoo.com
Calvo
María Isabel
MI
D000975:Antioxidants / Q000032:analysis; D005419:Flavonoids / Q000032:analysis*; D010636:Phenols / Q000032:analysis*; D010936:Plant Extracts / Q000737:chemistry*; D010944:Plants / Q000737:chemistry*; D059808:Polyphenols; D011540:Prussian Blue Reaction; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet / Q000295:instrumentation / Q000379:methods*
D000975:Antioxidants; D005419:Flavonoids; D010636:Phenols; D010936:Plant Extracts; D059808:Polyphenols