title: Upregulated BclG(L) expression enhances apoptosis of peripheral blood CD4+ T lymphocytes in patients with systemic lupus erythematosus.
abstract: Increased lymphocyte apoptosis has been suggested to contribute to the development of systemic lupus erythematosus (SLE), but the critical factors involved in the apoptotic pathways are still unknown. By long serial analysis of gene expression (LongSAGE) profiles and microarray analyses, a novel apoptosis-related gene BclG(L) expression was found significantly increased in peripheral blood CD4+ T cells of SLE patients, which was correlated with the enhanced CD4+ T cells apoptosis, anti-nuclear antibody (ANA) titer and proteinuria. In vitro, BclG(L) expression could be specially upregulated by SLE serum stimulation and positively correlated with induced CD4+ T cell apoptosis. Enforcing BclG(L) overexpression by lentivirus could directly enhance CD4+ T cell apoptosis, but these apoptosis-inducing effects could be partially inhibited by knockdown of BclG(L) expression. Collectively, these results indicate that increased BclG(L) expression may contribute to the aberrant CD4+ T cell apoptosis which causes an inappropriate immune response and impaired homeostasis in SLE.
authors.affiliation: Department of Dermatology, Southwest Hospital, PLA, Third Military Medical University, Chongqing 400038, China.
authors.forename: Na
authors.initials: N
authors.lastname: Luo
authors.forename: Yi
authors.initials: Y
authors.lastname: Wu
authors.forename: Yongwen
authors.initials: Y
authors.lastname: Chen
authors.forename: Zhao
authors.initials: Z
authors.lastname: Yang
authors.forename: Sheng
authors.initials: S
authors.lastname: Guo
authors.forename: Lei
authors.initials: L
authors.lastname: Fei
authors.forename: Di
authors.initials: D
authors.lastname: Zhou
authors.forename: Chengying
authors.initials: C
authors.lastname: Yang
authors.forename: Shengxi
authors.initials: S
authors.lastname: Wu
authors.forename: Bing
authors.initials: B
authors.lastname: Ni
authors.forename: Fei
authors.initials: F
authors.lastname: Hao
authors.forename: Yuzhang
authors.initials: Y
authors.lastname: Wu
mesh_terms: D000328:Adult; D000974:Antibodies, Antinuclear / Q000097:blood / Q000276:immunology; D017209:Apoptosis / Q000276:immunology*; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology* / Q000378:metabolism / Q000473:pathology; D015536:Down-Regulation / Q000235:genetics / Q000276:immunology; D005260:Female; D015870:Gene Expression / Q000276:immunology; D020869:Gene Expression Profiling; D006801:Humans; D008180:Lupus Erythematosus, Systemic / Q000276:immunology* / Q000378:metabolism / Q000473:pathology; D016131:Lymphocyte Subsets / Q000378:metabolism / Q000473:pathology; D008875:Middle Aged; D020411:Oligonucleotide Array Sequence Analysis; D011507:Proteinuria / Q000652:urine; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000235:genetics / Q000378:metabolism*; D034741:RNA, Small Interfering / Q000235:genetics; D044967:Serum / Q000276:immunology; D014162:Transfection; D015854:Up-Regulation / Q000235:genetics / Q000276:immunology*
chemical_list: D000974:Antibodies, Antinuclear; C428448:BCL2L14 protein, human; D019253:Proto-Oncogene Proteins c-bcl-2; D034741:RNA, Small Interfering