title: The janus face of resveratrol in astroglial cells.
abstract: Astroglial cells are key modulators of neuropathology events. Resveratrol, a redox-active compound present in grapes and wine, has a wide range of biological effects. The aim of this study was to investigate whether resveratrol is able to prevent hydrogen peroxide (H(2)O(2))-induced oxidative damage in C6 astroglial cells. We found that following a short oxidative insult (Model I-1 mM H(2)O(2)/30 min), resveratrol increased glutamate uptake (60%), glutamine synthetase (GS) (139%), glutathione (GSH) (120%), and S100B secretion (24%); and attenuated DCFH oxidation (34%) as compared to H(2)O(2) values. Under less intense (0.1 mM H(2)O(2)), but lasting (6 h) insult (Model II), resveratrol had an opposite effect, potentiating the H(2)O(2)-induced decrease in glutamate uptake (from 34 to 63%), in GS (from 22 to 50%), in GSH (from 22 to 54%), and also potentiating DCFH oxidation (from 24 to 38%). The transcription factor, NF-kappaB, was activated in both models. Cell morphology alterations were also observed in the presence of H(2)O(2) with process-bearing cells, accompanied by cell body retraction and actin reorganization. This effect was not prevented by resveratrol, but was prevented by lysophosphatidic acid (LPA), a specific upstream positive regulator of Rho A. In summary, these findings showed that resveratrol, a redox-active compound, was able to modulate important neurotrophic function of astroglial cells under different oxidative conditions.
authors.affiliation: Departamento de Bioquímica, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, Rua Ramiro Barcelos 2600 anexo, Porto Alegre, RS, 90035-003, Brazil.
authors.forename: André
authors.initials: A
authors.lastname: Quincozes-Santos
authors.forename: Patrícia
authors.initials: P
authors.lastname: Nardin
authors.forename: Daniela Fraga
authors.initials: DF
authors.lastname: de Souza
authors.forename: Daniel P
authors.initials: DP
authors.lastname: Gelain
authors.forename: José Cláudio
authors.initials: JC
authors.lastname: Moreira
authors.forename: Alexandra
authors.initials: A
authors.lastname: Latini
authors.forename: Carlos-Alberto
authors.initials: CA
authors.lastname: Gonçalves
authors.forename: Carmem
authors.initials: C
authors.lastname: Gottfried
mesh_terms: D000199:Actins / Q000378:metabolism; D000704:Analysis of Variance; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001253:Astrocytes / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D004347:Drug Interactions; D024202:Electrophoretic Mobility Shift Assay / Q000379:methods; D005910:Glioma; D005974:Glutamate-Ammonia Ligase / Q000378:metabolism; D018698:Glutamic Acid / Q000378:metabolism; D005978:Glutathione / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000494:pharmacology; D016328:NF-kappa B / Q000378:metabolism; D009414:Nerve Growth Factors / Q000378:metabolism; D016877:Oxidants / Q000494:pharmacology; D010703:Phorbol Esters / Q000494:pharmacology; D011419:Propidium; D051381:Rats; D017382:Reactive Oxygen Species / Q000378:metabolism; D000077185:Resveratrol; D064568:S100 Calcium Binding Protein beta Subunit; D009418:S100 Proteins / Q000378:metabolism; D013267:Stilbenes / Q000494:pharmacology*
chemical_list: D000199:Actins; D000975:Antioxidants; D016328:NF-kappa B; D009414:Nerve Growth Factors; D016877:Oxidants; D010703:Phorbol Esters; D017382:Reactive Oxygen Species; D064568:S100 Calcium Binding Protein beta Subunit; D009418:S100 Proteins; C579158:S100b protein, rat; D013267:Stilbenes; D011419:Propidium; D018698:Glutamic Acid; D006861:Hydrogen Peroxide; D005974:Glutamate-Ammonia Ligase; D005978:Glutathione; D000077185:Resveratrol