Cross-reactivity of Schistosoma mansoni-Fasciola gigantica influenced by saponins.
The aim of the present work was to investigate the Schistosoma mansoni and Fasciola gigantica cross-reactivity between adult worms and egg homogenates of the parasites. Immunoprophylactic effects of crude Schistosoma mansoni worms and egg antigens mixed with or without saponins extracted from Atriplex nummularia were studied followed by challenge with 80 cercariae of Schistosoma mansoni. Our results showed that post 1st immunization with schistosome egg antigens (SEA) there was a significant change (P approximately 0.05) in the IgM levels against Fasciola egg homogenate (FgEH) without saponins. Post 2nd immunization with SEA mixed with saponins the levels of IgM increased significantly (P approximately 0.05) against Fasciola worm homogenate (FgWH) as compared with a non-immunized group. Post 2nd immunization the level of IgG was significantly elevated (P approximately 0.05) by SEA mixed with saponins against FgWH. Post 2nd immunizations with SEA mixed with saponins showed a significant change (P approximately 0.05) in IgG levels against FgEH. These results clearly demonstrated that there is a cross-reactivity between Schistosoma mansoni eggs and Fasciola gigantica worms and eggs. Saponins were found to be immunostimulatory adjuvants in our study.
Maghraby
Amany Sayed
AS
Therapeutical Chemistry Department, Infectious Diseases and Immunology Group, Centre of Excellence for Advanced Sciences (CEAS), the Road to Nobel Project at the National Research Centre, Dokki, 12311 Cairo, Egypt. maghraby_amany@yahoo.com
Shaker
Kamel H
KH
Gaber
Hanaa M
HM
D000818:Animals; D000941:Antigens / Q000276:immunology*; D003429:Cross Reactions; D004531:Eggs; D005209:Fasciola / Q000187:drug effects / Q000276:immunology*; D007114:Immunization; D007075:Immunoglobulin M / Q000276:immunology; D051379:Mice; D008958:Models, Molecular; D012503:Saponins / Q000737:chemistry / Q000494:pharmacology*; D012550:Schistosoma mansoni / Q000187:drug effects / Q000276:immunology*
D000941:Antigens; D007075:Immunoglobulin M; D012503:Saponins