title: Systematic analysis of biochemical performance and the microbial community of an activated sludge process using ozone-treated sludge for sludge reduction.
abstract: Two lab-scale bioreactors (reactors 1 and 2) were employed to examine the changes in biological performance and the microbial community of an activated sludge process fed with ozonated sludge for sludge reduction. During the 122 d operation, the microbial activities and community in the two reactors were evaluated. The results indicated that, when compared with the conventional reactor (reactor 1), the reactor that was fed with the ozonated sludge (reactor 2) showed good removal of COD, TN and cell debris, without formation of any excess sludge. In addition, the protease activity and intracellular ATP concentration of reactor 2 were increased when compared to reactor 1, indicating that reactor 2 had a better ability to digest proteins and cell debris. DGGE analysis revealed that the bacterial communities in the two reactors were different, and that the dissimilarity of the bacterial population was nearly 40%. Reactor 2 also contained more protozoa and metazoa, which could graze on the ozone-treated sludge debris directly.
authors.affiliation: Department of Chemical Engineering, Tsinghua University, Beijing 100084, China.
authors.forename: Sang-Tian
authors.initials: ST
authors.lastname: Yan
authors.forename: Hao
authors.initials: H
authors.lastname: Zheng
authors.forename: An
authors.initials: A
authors.lastname: Li
authors.forename: Xue
authors.initials: X
authors.lastname: Zhang
authors.forename: Xin-Hui
authors.initials: XH
authors.lastname: Xing
authors.forename: Li-Bing
authors.initials: LB
authors.lastname: Chu
authors.forename: Guoji
authors.initials: G
authors.lastname: Ding
authors.forename: Xu-Lin
authors.initials: XL
authors.lastname: Sun
authors.forename: Benjamin
authors.initials: B
authors.lastname: Jurcik
mesh_terms: D000251:Adenosine Triphosphatases / Q000378:metabolism; D000818:Animals; D001419:Bacteria / Q000235:genetics / Q000378:metabolism*; D020029:Base Pairing; D001673:Biodegradation, Environmental; D044822:Biodiversity; D019149:Bioreactors / Q000382:microbiology / Q000469:parasitology; D004586:Electrophoresis; D056890:Eukaryota; D042541:Intracellular Space / Q000378:metabolism; D010126:Ozone / Q000737:chemistry*; D010447:Peptide Hydrolases / Q000378:metabolism; D010802:Phylogeny; D012722:Sewage / Q000382:microbiology*; D014865:Waste Disposal, Fluid
chemical_list: D012722:Sewage; D010126:Ozone; D010447:Peptide Hydrolases; D000251:Adenosine Triphosphatases