title: Collection and culture of alveolar bone marrow multipotent mesenchymal stromal cells from older individuals.
abstract: In this work, we examined the culture condition of alveolar bone marrow multipotent mesenchymal stromal cells (ABMMSCs), aiming to apply regenerative therapy to older periodontitis patients. To better understand the character of cultured cells from alveolar bone marrow, the expression profiles of well-known genes and their responses to the induction of osteogenic, chondrogenic, or adipogenic differentiation were examined. Using alpha MEM-based culture, ABMMSCs could be obtained from older individuals than in previous reports. Interestingly, ABMMSCs expressing Klf4 were able to differentiate into osteoblasts. The prediction of differentiation potential by Klf4 could be a useful guide for further improvement of the culture conditions required to culture ABMMSCs derived from older individuals.
authors.affiliation: Department of Pediatric Dentistry, Nihon University School of Dentistry at Matsudo, Matsudo, Chiba, Japan.
authors.forename: Juan
authors.initials: J
authors.lastname: Han
authors.forename: Hironori
authors.initials: H
authors.lastname: Okada
authors.forename: Hideki
authors.initials: H
authors.lastname: Takai
authors.forename: Youhei
authors.initials: Y
authors.lastname: Nakayama
authors.forename: Takahide
authors.initials: T
authors.lastname: Maeda
authors.forename: Yorimasa
authors.initials: Y
authors.lastname: Ogata
mesh_terms: D000539:Alveolar Process / Q000166:cytology*; D001854:Bone Marrow Cells / Q000166:cytology; D018929:Cell Culture Techniques / Q000379:methods; D002454:Cell Differentiation; D005260:Female; D006801:Humans; D000090062:Kruppel-Like Factor 4; D051741:Kruppel-Like Transcription Factors; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D008875:Middle Aged; D039902:Multipotent Stem Cells / Q000166:cytology; D010006:Osteoblasts / Q000166:cytology; D010518:Periodontitis / Q000628:therapy; D044968:Regenerative Medicine; D017154:Stromal Cells / Q000166:cytology*
chemical_list: C000715467:KLF4 protein, human; D000090062:Kruppel-Like Factor 4; D051741:Kruppel-Like Transcription Factors