title: Screening of antimutagenicity via antioxidant activity in different extracts from the flowers of Phlomis crinita Cav. ssp mauritanica munby from the center of Tunisia.
abstract: For centuries, plants have been used in traditional medicines, and there has been recent interest in the chemopreventive properties of compounds derived from plants. In the present study, we investigated the free-radical-scavenging, antioxidant, and antimutagenic potential of polar extracts from Phlomis crinita Cav. flowers. Ethyl acetate, chloroform, and methanol extracts were prepared from powdered Phlomis flowers and characterized for the presence of tannins, flavonoids, iridoids, sterols, cardiac glycosides, and anthraquinones. All the extracts showed increased activity in scavenging the ABTS free radical, but only ethyl acetate and methanol extracts were active in scavenging the superoxide anion generated by the xanthine/xanthine oxidase system. In addition, all the extracts significantly decreased the mutagenicity induced by 2-aminoanthracene in the presence of a metabolizing homogenate (S9) and methyl methane sulfonate in the absence of metabolizing system, using the Ames test with Salmonella typhimurium TA102 and TA104. The present study indicates that extracts of P. crinita flowers are a significant source of compounds with antigenotoxic and antioxidant activity (most likely phenolic compounds) and thus may be useful candidates for chemoprevention studies.
authors.affiliation: Unité de Recherche "Pharmacognosie/Biologie Moléculaire" (99/UR/07-03), Faculté de Pharmacie, Monastir, Tunisie.
authors.forename: Afef
authors.initials: A
authors.lastname: Dellai
authors.forename: Hedi Ben
authors.initials: HB
authors.lastname: Mansour
authors.forename: Ilef
authors.initials: I
authors.lastname: Limem
authors.forename: Ines
authors.initials: I
authors.lastname: Bouhlel
authors.forename: Mohamed Ben
authors.initials: MB
authors.lastname: Sghaier
authors.forename: Jihed
authors.initials: J
authors.lastname: Boubaker
authors.forename: Kamel
authors.initials: K
authors.lastname: Ghedira
authors.forename: Leila
authors.initials: L
authors.lastname: Chekir-Ghedira
mesh_terms: D000818:Animals; D000873:Anthracenes / Q000633:toxicity; D016587:Antimutagenic Agents / Q000494:pharmacology*; D035264:Flowers / Q000737:chemistry; D016166:Free Radical Scavengers / Q000494:pharmacology*; D008741:Methyl Methanesulfonate / Q000633:toxicity; D009153:Mutagens / Q000633:toxicity; D031345:Phlomis / Q000737:chemistry*; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology*; D051381:Rats; D000091243:Ribosomal Protein S9; D012269:Ribosomal Proteins / Q000187:drug effects / Q000378:metabolism; D012486:Salmonella typhimurium / Q000187:drug effects / Q000235:genetics; D012997:Solvents / Q000737:chemistry; D013481:Superoxides / Q000378:metabolism; D014416:Tunisia; D014969:Xanthine Oxidase / Q000037:antagonists & inhibitors
chemical_list: D000873:Anthracenes; D016587:Antimutagenic Agents; D016166:Free Radical Scavengers; D009153:Mutagens; D010936:Plant Extracts; D000091243:Ribosomal Protein S9; D012269:Ribosomal Proteins; D012997:Solvents; D013481:Superoxides; C006593:2-anthramine; D008741:Methyl Methanesulfonate; D014969:Xanthine Oxidase