title: First isolation and characterization of Lactococcus garvieae from Brazilian Nile tilapia, Oreochromis niloticus (L.), and pintado, Pseudoplathystoma corruscans (Spix & Agassiz).
abstract: Lactococcus garvieae infection in cultured Nile tilapia, Oreochromis niloticus (L.), and pintado, Pseudoplathystoma corruscans (Spix & Agassiz), from Brazil is reported. The commercial bacterial identification system, Biolog Microlog, confirmed the identity of L. garvieae. Infectivity trials conducted in Nile tilapia using Brazilian Nile tilapia L. garvieae isolates resulted in a median lethal dose-50 of 1.4 x 10(5) colony-forming units (CFU)/fish. This is the first evidence of the presence of this pathogen from Brazilian fish. In addition, this is the first report of L. garvieae infection in either Nile tilapia or pintado. Collectively, this evidence expands the geographical range of fish hosts, number of fish hosts harbouring L. garvieae and carbon source utilization by L. garvieae fish isolates. Furthermore, the Biolog system may be an alternative technique to polymerase chain reaction for the identification of L. garvieae and discrimination between closely related bacterial species.
authors.affiliation: USDA, ARS Aquatic Animal Health Laboratory, Chestertown, MD 21620, USA. Joyce.Evans@ars.usda.gov
authors.forename: J J
authors.initials: JJ
authors.lastname: Evans
authors.forename: P H
authors.initials: PH
authors.lastname: Klesius
authors.forename: C A
authors.initials: CA
authors.lastname: Shoemaker
mesh_terms: D000818:Animals; D015373:Bacterial Typing Techniques / Q000662:veterinary; D001938:Brazil; D002397:Catfishes / Q000382:microbiology*; D023681:Cichlids / Q000382:microbiology*; D005393:Fish Diseases / Q000382:microbiology* / Q000401:mortality; D005399:Fishes; D016908:Gram-Positive Bacterial Infections / Q000382:microbiology / Q000401:mortality / Q000662:veterinary*; D017045:Lactococcus / Q000145:classification / Q000302:isolation & purification* / Q000378:metabolism / Q000472:pathogenicity / Q000502:physiology*; D013997:Time Factors