BacMam: versatile gene delivery technology for GPCR assays.
BacMam viruses are modified baculoviruses that contain mammalian expression cassettes for viral gene delivery and transient expression in mammalian cells. They are easily, inexpensively, and rapidly generated and provide a versatile solution for G protein-coupled receptor (GPCR) cell-based assay development. Using BacMam technology, target gene expression levels are easily controlled and simultaneous delivery of multiple genes is possible, for example, coexpression of a receptor and a G protein or a reporter gene. BacMam viruses are compatible with the GPCR cell-based assay formats typically used in high-throughput screening and provide an unparalleled level of experimental flexibility that is simply not possible when using stable recombinant cell lines.
Davenport
Elizabeth A
EA
Molecular Discovery Research, Biological Reagents and Assay Development, GlaxoSmithKline R&D, Collegeville, PA, USA.
Nuthulaganti
Parvathi
P
Ames
Robert S
RS
D016367:Baculoviridae / Q000235:genetics*; D001859:Bone Neoplasms / Q000235:genetics / Q000378:metabolism*; D018014:Gene Transfer Techniques; D005822:Genetic Vectors; D006801:Humans; D012516:Osteosarcoma / Q000235:genetics / Q000378:metabolism*; D043562:Receptors, G-Protein-Coupled / Q000235:genetics* / Q000378:metabolism; D014161:Transduction, Genetic; D014407:Tumor Cells, Cultured
D043562:Receptors, G-Protein-Coupled