title: Generation and characterization of monoclonal antibodies specific to Drosophila presenilin.
abstract: The development of a monoclonal antibody (MAb) specific to Drosophila presenilin (Psn) proteins in vivo was the major aim of this study, since the absence of specific antibodies recognizing Psn proteins hampered our progress in understanding Psn functions during development, differentiation, and pathogenesis. By dot blot and immunofluorescence screenings, we found that MAb Psn2G6 specifically recognized Psn proteins in wing imaginal discs and brains of wild-type control W1118 larvae. MAb Psn2G6 also transgenically expressed a long form of wild-type Psn (Psn + 14 WT) proteins in wing imaginal discs of two independent transgenic lines. Transgenic expression of Psn + 14 WT proteins in psn(B3) larvae completely rescued the expression patterns of Psn proteins and the development of wing imaginal discs. In addition, neural hyperplasia observed in wing imaginal discs of psn(B3) larvae was also suppressed.
authors.affiliation: Ilsong Institute of Life Science, Hallym University , Dongan-gu, Anyang, Gyeonggi-do, Republic of Korea.
authors.forename: Ho Thi Thu
authors.initials: HT
authors.lastname: Cuc
authors.forename: Jong Bok
authors.initials: JB
authors.lastname: Seo
authors.forename: Jin Kyu
authors.initials: JK
authors.lastname: Choi
authors.forename: Won Tae
authors.initials: WT
authors.lastname: Kim
authors.forename: Seok Jou
authors.initials: SJ
authors.lastname: Park
authors.forename: Dae Weon
authors.initials: DW
authors.lastname: Lee
authors.forename: Yong Sun
authors.initials: YS
authors.lastname: Kim
authors.forename: Mark E
authors.initials: ME
authors.lastname: Fortini
authors.forename: Young Ho
authors.initials: YH
authors.lastname: Koh
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D030801:Animals, Genetically Modified; D000911:Antibodies, Monoclonal / Q000276:immunology*; D004330:Drosophila; D005455:Fluorescent Antibody Technique; D008969:Molecular Sequence Data; D053763:Presenilins / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D053763:Presenilins