title: In vitro photodynamic properties of methylene blue in a combination with laser illumination at 630 nm concerning Candida albicans.
abstract: The study had to define influence of combined applications of laser radiation and methylene blue (MB) on pathogenic culture of Candida albicans in vitro. The experimental study was done at standard techniques of method of cultivations in a broth. The laser irradiation of cultures was done at once after addition of MB in concentration 0.05%, 0.1% and 0.2%. During studying action of MB in dark, influence of MB to the growth of test-shtam without laser radiation, were prepared fluid Gissa's broth with glucose without Andrede's indicator. Activation of MB was done by laser with wave length 630 nm during 3 or 5 min. All experiments were passed in 4 parallels and 3 repeats. Maximal suppression of growth of microorganisms was noted in group with using 0.1% MB with laser radiation 3 minutes without centrifugation after 24 hours. Maximal suppression of growth was noted in group after centrifugation with 0.05% MB with exposure of laser 3 min. after 48 hours. Sensitivity of pathogenic culture of Candida albicans to application of B and laser raises accordingly to increase of concentration of MB.
authors.affiliation: State Institution The Filatov' s Institute of Eye Diseases and Tissue Therapy of AMS of Ukraine, Laboratory of Microbiology, Department of Uveitis.
authors.forename: Nataliya
authors.initials: N
authors.lastname: Pasyechnikova
authors.forename: Olexandra
authors.initials: O
authors.lastname: Zborovskaya
authors.forename: Taras
authors.initials: T
authors.lastname: Kustrin
mesh_terms: D002176:Candida albicans / Q000254:growth & development* / Q000528:radiation effects*; D007834:Lasers; D008029:Lighting; D008751:Methylene Blue / Q000737:chemistry*; D010775:Photic Stimulation
chemical_list: D008751:Methylene Blue