Effects of oxytetracycline and sulfachloropyridazine residues on the reductive activity of Shewanella decolorationis S12.
Effects of oxytetracycline (OTC) and sulfachloropyridazine (SCP), two of the widely used antibiotics in livestock production, on beneficial environmental microorganisms were studied. Shewanella decolorationis S12 was selected as the target bacteria for the role in reduction of Fe(III) and dye under anaerobic conditions. The results showed that the antibiotics significantly inhibited Fe(III) reduction and dye decoloration in the reduction system. The rates of Fe(II) formed (-r) were 3.6 and 0.2 mg/L/day for the OTC concentrations of 0-1 mg/L and 1-50 mg/L, respectively, with 1 mg/L as the turning point of the inhibition effect. The turning point of inhibition effect was much higher for SCP treatments, at 4 mg/L. The results also showed higher production values for adsorbed Fe(II) than soluble Fe(II) in OTC treatments, but the reverse occurred in the SCP treatments. The difference between the treatments could be due to higher sorption coefficients of OTC as compared to SCP. Transmission electron micrographs showed changes in cell structures of S. decolorationis S12 grown in medium with OTC. Detached cell walls and large vacuoles in internal cell contents were found in OTC-treated cells. The results of the present study indicated that the inhibition of antibiotic on the reduction activity of S. decolorationis S12 may be due to a decrease in live S. decolorationis S12 population and/or damages of their cell structure in this reduction system.
Wang
Yan
Y
Guangdong Institute of Eco-Environmental and Soil Sciences, Guangzhou 510650, PR China.
Wang
Lusong
L
Li
Fangbai
F
Liang
Juanboo
J
Li
Yongtao
Y
Dai
Jun
J
Loh
Teck-Chwen
TC
Ho
Yin-Wan
YW
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D004396:Coloring Agents / Q000378:metabolism; D005296:Ferrous Compounds / Q000378:metabolism; D046529:Microscopy, Electron, Transmission; D010084:Oxidation-Reduction; D010118:Oxytetracycline / Q000494:pharmacology*; D020592:Shewanella / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure; D012988:Soil Microbiology; D013410:Sulfachlorpyridazine / Q000494:pharmacology*
D000900:Anti-Bacterial Agents; D004396:Coloring Agents; D005296:Ferrous Compounds; D013410:Sulfachlorpyridazine; D010118:Oxytetracycline