title: Assessment of the ability of a fourth-generation immunoassay for human immunodeficiency virus (HIV) antibody and p24 antigen to detect both acute and recent HIV infections in a high-risk setting.
abstract: An immunoassay (IA) that simultaneously detects both antibody to human immunodeficiency virus (HIV) and HIV p24 antigen (Architect HIV Ag/Ab Combo) was evaluated for its ability to detect HIV infection by using a panel of specimens collected from individuals recently infected with HIV type 1 (HIV-1). This IA was found to be capable of detecting the majority (89%) of infections, including 80% of those considered acute infections based on the presence of HIV RNA and the lack of detectable antibody to HIV. Substantial improvements in detection of recent infections by the Architect HIV Ag/Ab Combo relative to previous generations of IAs as well as the capacity to detect acute infections have important implications for HIV prevention strategies.
authors.affiliation: San Francisco Department of Public Health, CA 94102, USA. Mark_Pandori@sfdph.org
authors.forename: Mark W
authors.initials: MW
authors.lastname: Pandori
authors.forename: John
authors.initials: J
authors.lastname: Hackett
authors.forename: Brian
authors.initials: B
authors.lastname: Louie
authors.forename: Ana
authors.initials: A
authors.lastname: Vallari
authors.forename: Teri
authors.initials: T
authors.lastname: Dowling
authors.forename: Sally
authors.initials: S
authors.lastname: Liska
authors.forename: Jeffrey D
authors.initials: JD
authors.lastname: Klausner
mesh_terms: D000956:Antigens, Viral / Q000097:blood*; D015483:HIV Antibodies / Q000097:blood*; D016655:HIV Core Protein p24 / Q000097:blood*; D015658:HIV Infections / Q000175:diagnosis*; D015497:HIV-1 / Q000276:immunology / Q000302:isolation & purification*; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D012680:Sensitivity and Specificity
chemical_list: D000956:Antigens, Viral; D015483:HIV Antibodies; D016655:HIV Core Protein p24