Multiplexed LC-MS/MS analysis of horse plasma proteins to study doping in sport.
The development of protein biomarkers for the indirect detection of doping in horse is a potential solution to doping threats such as gene and protein doping. A method for biomarker candidate discovery in horse plasma is presented using targeted analysis of proteotypic peptides from horse proteins. These peptides were first identified in a novel list of the abundant proteins in horse plasma. To monitor these peptides, an LC-MS/MS method using multiple reaction monitoring was developed to study the quantity of 49 proteins in horse plasma in a single run. The method was optimised and validated, and then applied to a population of race-horses to study protein variance within a population. The method was finally applied to longitudinal time courses of horse plasma collected after administration of an anabolic steroid to demonstrate utility for hypothesis-driven discovery of doping biomarker candidates.
Barton
Chris
C
HFL Sport Science, Quotient Bioresearch Ltd, Fordham, Cambridgeshire, UK. chris.barton@quotientbioresearch.com
Beck
Paul
P
Kay
Richard
R
Teale
Phil
P
Roberts
Jane
J
D000818:Animals; D015415:Biomarkers / Q000032:analysis / Q000378:metabolism; D001798:Blood Proteins / Q000032:analysis* / Q000378:metabolism; D002853:Chromatography, Liquid / Q000379:methods*; D004300:Doping in Sports; D006736:Horses / Q000097:blood*; D016014:Linear Models; D008297:Male; D020543:Proteome / Q000032:analysis / Q000378:metabolism; D015203:Reproducibility of Results; D053719:Tandem Mass Spectrometry / Q000379:methods*; D013739:Testosterone / Q000008:administration & dosage; D013997:Time Factors
D015415:Biomarkers; D001798:Blood Proteins; D020543:Proteome; D013739:Testosterone