title: Bilberry extract reduces UVA-induced oxidative stress in HaCaT keratinocytes: a pilot study.
abstract: Exposure to UVA radiation is known to cause many adverse biological effects by inducing the stricken cells to produce reactive oxygen species (ROS). In recent years the use of botanicals has received considerable interest in the skin protection. Bilberry (Vaccinium myrtillus L.) fruit contains several polyphenols with strong antioxidant and anti-inflammatory properties. In this study we evaluated potential UVA preventive effect of V. myrtillus fruit extract (VME; anthocyanins, 25% w/w) in HaCaT keratinocytes. Pre-treatment (1 h) or post-treatment (4 h) of HaCaT with VME resulted in attenuation of UVA-caused damage. Application of the extract significantly reduced UVA-stimulated ROS formation in keratinocytes. VME also prevented/reduced UVA-caused peroxidation of membrane lipids and depletion of intracellular GSH. The observed cytoprotective effect may be linked to the antioxidant activity of the plant constituents, namely anthocyanins.
authors.affiliation: Department of Medical Chemistry and Biochemistry, Faculty of Medicine and Dentistry, Palacký University, Olomouc, Czech Republic. alf.svoboda@seznam
authors.forename: Alena
authors.initials: A
authors.lastname: Svobodová
authors.forename: Jana
authors.initials: J
authors.lastname: Rambousková
authors.forename: Daniela
authors.initials: D
authors.lastname: Walterová
authors.forename: Jitka
authors.initials: J
authors.lastname: Vostalová
mesh_terms: D000872:Anthocyanins / Q000494:pharmacology*; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects / Q000528:radiation effects; D004355:Drug Stability; D016166:Free Radical Scavengers / Q000494:pharmacology; D005978:Glutathione / Q000378:metabolism; D006801:Humans; D015603:Keratinocytes / Q000187:drug effects*; D015227:Lipid Peroxidation / Q000187:drug effects; D018384:Oxidative Stress / Q000187:drug effects* / Q000528:radiation effects*; D010936:Plant Extracts / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000378:metabolism; D014466:Ultraviolet Rays; D029800:Vaccinium myrtillus / Q000737:chemistry*
chemical_list: D000872:Anthocyanins; D016166:Free Radical Scavengers; D010936:Plant Extracts; D017382:Reactive Oxygen Species; C073305:Vaccinium myrtillus extract; D005978:Glutathione