Combination of methods for in vitro study of antioxidant properties of chemical compounds.
We proposed a combination of methods to study antioxidant properties of compounds, including evaluation of the capacity of the test preparations to inhibit peroxidation of unsaturated lipids (in model systems with oxidation of ethyl oleate; aqueous emulsion medium) and low-density lipoproteins (in the presence of transition metal ions), generation of superoxide anion radical (system of lucigenin, xanthine oxidase, and xanthine) and NO(*)/ONOO(-) (system of SIN-1 and lucigenin), and induction of respiratory burst in blood granulocytes (luminol-induced and lucigenin-induced reaction after zymosan stimulation). In vitro study showed that the antioxidant properties of synthetic water-soluble phenols depend strongly on masking of the phenol OH group and nature of the ionogenic fragment in the p-propyl substituent.
Menshchikova
E B
EB
Research Center of Clinical and Experimental Medicine, Siberian Division of the Russian Academy of Medical Sciences. Novosibirsk State Pedagogical University, Russia. lemen@soramn.ru
Zenkov
N K
NK
Kandalintseva
N V
NV
Prosenko
A E
AE
D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D006098:Granulocytes / Q000187:drug effects / Q000378:metabolism; D008077:Lipoproteins, LDL / Q000378:metabolism; D049408:Luminescent Agents / Q000494:pharmacology; D008165:Luminol / Q000494:pharmacology; D010084:Oxidation-Reduction; D051381:Rats; D016897:Respiratory Burst / Q000187:drug effects; D013481:Superoxides / Q000378:metabolism; D019820:Xanthine / Q000378:metabolism; D014969:Xanthine Oxidase / Q000378:metabolism; D015054:Zymosan / Q000494:pharmacology
D000975:Antioxidants; D008077:Lipoproteins, LDL; D049408:Luminescent Agents; D013481:Superoxides; D019820:Xanthine; D008165:Luminol; D015054:Zymosan; D014969:Xanthine Oxidase