title: The Hippo pathway regulates apical-domain size independently of its growth-control function.
abstract: The Hippo pathway, identified in Drosophila and conserved in vertebrates, regulates tissue growth by promoting cell cycle exit and apoptosis. In addition to their well-characterised overproliferation phenotype, adult Drosophila epithelial cells mutant for the kinases Hippo and Warts have hypertrophic apical domains. Here we examine the molecular basis of this apical hypertrophy and its impact on cell proliferation. In the wing imaginal disc epithelium, we observe increased staining for members of the apical polarity complexes aPKC and Crumbs as well as adherens junction components when Hippo activity is compromised, while basolateral markers are not affected. This increase in apical proteins is correlated with a hypertrophy of the apical domain and adherens junctions. The cell surface localisation of the Notch receptor is also increased in mutant clones, opening the possibility that aberrant receptor signalling may participate in overgrowth of hpo-deficient tissue. Interestingly, however, although the polarity determinant Crumbs is required for the accumulation of apical proteins, this does not appear to significantly contribute to the overproliferation defect elicited by loss of Hippo signalling. Therefore, Hippo signalling controls growth and apical domain size by distinct mechanisms.
authors.affiliation: Apoptosis and Proliferation Control Laboratory, Cancer Research UK, London Research Institute, 44 Lincoln's Inn Fields, London WC2A 3PX, UK.
authors.forename: Alice
authors.initials: A
authors.lastname: Genevet
authors.forename: Cédric
authors.initials: C
authors.lastname: Polesello
authors.forename: Ken
authors.initials: K
authors.lastname: Blight
authors.forename: Francesca
authors.initials: F
authors.lastname: Robertson
authors.forename: Lucy M
authors.initials: LM
authors.lastname: Collinson
authors.forename: Franck
authors.initials: F
authors.lastname: Pichaud
authors.forename: Nicolas
authors.initials: N
authors.lastname: Tapon
mesh_terms: D022005:Adherens Junctions / Q000378:metabolism; D000818:Animals; D017209:Apoptosis; D015820:Cadherins / Q000378:metabolism; D002462:Cell Membrane / Q000378:metabolism; D016764:Cell Polarity; D049109:Cell Proliferation; D004330:Drosophila / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D029721:Drosophila Proteins / Q000172:deficiency / Q000235:genetics / Q000378:metabolism*; D004847:Epithelial Cells / Q000378:metabolism*; D005838:Genotype; D006984:Hypertrophy; D047908:Intracellular Signaling Peptides and Proteins / Q000172:deficiency / Q000235:genetics / Q000378:metabolism*; D008565:Membrane Proteins / Q000378:metabolism; D009154:Mutation; D009687:Nuclear Proteins / Q000378:metabolism; D010641:Phenotype; D011493:Protein Kinase C / Q000378:metabolism; D017346:Protein Serine-Threonine Kinases / Q000172:deficiency / Q000235:genetics / Q000378:metabolism*; D051880:Receptors, Notch / Q000378:metabolism; D015398:Signal Transduction / Q000235:genetics; D015534:Trans-Activators / Q000378:metabolism; D014921:Wings, Animal / Q000254:growth & development / Q000378:metabolism*; D000091102:YAP-Signaling Proteins; D000099317:Hippo Kinases
chemical_list: D015820:Cadherins; D029721:Drosophila Proteins; D047908:Intracellular Signaling Peptides and Proteins; D008565:Membrane Proteins; D009687:Nuclear Proteins; D011493:Protein Kinase C; D017346:Protein Serine-Threonine Kinases; D051880:Receptors, Notch; D015534:Trans-Activators; D000091102:YAP-Signaling Proteins; C497759:N protein, Drosophila; C503814:Yki protein, Drosophila; C064360:crb protein, Drosophila; C501889:shg protein, Drosophila; C499474:hpo protein, Drosophila; D000099317:Hippo Kinases; C115015:PKC-3 protein