Expression and functional roles of Smad1 and BMPR-IB in glioma development.
Here we report the negative correlation of phosphorylation of Smad1 and BMPR-IB expression with the development of human glioma. Western blot analysis showed that expression of both phospho-Smad1/5/8 and BMPR-IB were decreased in malignant glioma tissues compared with normal brain tissues. Kaplan-Meier survival curves revealed that lower expression ratio of phospho-Smad1/5/8 to Smad1 expression significantly correlates with poor patient survival. Transient transfection of BMPR-IB activates Smad1 signaling and induces differentiation and apoptosis of U251 and U87 glioblastoma cells. The effects could be blocked by cotransfection of Smad6. These results might provide new molecular marker and target for glioma diagnosis and therapy.
Liu
Shuang
S
Department of Brain Protection and Plasticity Research, Beijing Institute of Basic Medical Sciences, Beijing, China.
Tian
Zengmin
Z
Yin
Feng
F
Zhang
Peng
P
W
Yanrui
Y
Ding
Xuefeng
X
Wu
Haitao
H
Wu
Yan
Y
Peng
Xiaozhong
X
Yuan
Jiangang
J
Qiang
Boqin
B
Fan
Wenhong
W
Fan
Ming
M
D017209:Apoptosis; D001254:Astrocytoma / Q000235:genetics / Q000378:metabolism* / Q000401:mortality; D052005:Bone Morphogenetic Protein Receptors, Type I / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology*; D001921:Brain / Q000378:metabolism; D001932:Brain Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000401:mortality; D045744:Cell Line, Tumor; D003864:Depression, Chemical; D015972:Gene Expression Regulation, Neoplastic; D005909:Glioblastoma / Q000235:genetics / Q000378:metabolism* / Q000401:mortality; D006801:Humans; D053208:Kaplan-Meier Estimate; D009363:Neoplasm Proteins / Q000235:genetics / Q000378:metabolism*; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000502:physiology; D010766:Phosphorylation; D011379:Prognosis; D011499:Protein Processing, Post-Translational; D051898:Smad1 Protein / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D051902:Smad5 Protein / Q000378:metabolism; D051904:Smad8 Protein / Q000378:metabolism; D016019:Survival Analysis; D014162:Transfection
D009363:Neoplasm Proteins; D009419:Nerve Tissue Proteins; C497399:SMAD1 protein, human; C497797:SMAD5 protein, human; C107514:SMAD9 protein, human; D051898:Smad1 Protein; D051902:Smad5 Protein; D051904:Smad8 Protein; D052005:Bone Morphogenetic Protein Receptors, Type I