Dynamic disorder in horseradish peroxidase observed with total internal reflection fluorescence correlation spectroscopy.
This paper discusses the application of objective-type total internal reflection fluorescence correlation spectroscopy (TIR-FCS) to the study of the kinetics of immobilized horseradish peroxidase on a single molecule level. Objective-type TIR-FCS combines the advantages of FCS with TIRF microscopy in a way that allows for simultaneous ultra-sensitive spectroscopic measurements using a single-point detector and convenient localization of single molecules on a surface by means of parallel imaging.
Hassler
Kai
K
Rigler
Per
P
Blom
Hans
H
Rigler
Rudolf
R
Widengren
Jerker
J
Lasser
Theo
T