Absorption of andrographolides from Andrographis paniculata and its effect on CCl(4)-induced oxidative stress in rats.
A simple and validated high-performance liquid chromatography (HPLC) method with UV detection has been used to determine the content of andrographolide (AP) and 14-deoxy-11,12-didehydroandrographolide (DIAP) in rat plasma after oral dose of methanol extract (1 g/kg body weight) of Andrographis paniculata leaf. An increase in plasma concentration of AP and DIAP was observed from 30 min to 3 h after oral administration of the extract. The maximum plasma concentrations of AP and DIAP were 1.42+/-0.09 microg/ml and 1.31+/-0.04 microg/ml, respectively. Fourteen days oral treatment of rats with the methanol extract (1 g/kg body weight) followed by CCl(4) administration preserved catalase (CAT), and superoxide dismutase (SOD) activities in erythrocytes, whereas plasma lipid peroxidation, alanine transaminase (ALT) and aspartate transaminase (AST) activities were restored to values comparable with control values. Treatment of rats with CCl(4) did not showed significant alteration (p>0.05) in plasma total antioxidant status (TAS) as compare to values of control group.
Akowuah
G A
GA
School of Pharmacy, UCSI University, Cheras, Kuala Lumpur, Malaysia. wuahmy@yahoo.com
Zhari
I
I
Mariam
A
A
Yam
M F
MF
D000410:Alanine Transaminase / Q000097:blood; D035685:Andrographis / Q000737:chemistry*; D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000097:blood / Q000493:pharmacokinetics*; D001219:Aspartate Aminotransferases / Q000097:blood; D002252:Carbon Tetrachloride Poisoning / Q000188:drug therapy* / Q000378:metabolism; D002374:Catalase / Q000378:metabolism; D004224:Diterpenes / Q000097:blood / Q000493:pharmacokinetics*; D004912:Erythrocytes / Q000187:drug effects / Q000201:enzymology; D015227:Lipid Peroxidation / Q000187:drug effects; D018384:Oxidative Stress / Q000187:drug effects*; D010936:Plant Extracts / Q000493:pharmacokinetics; D010946:Plants, Medicinal / Q000737:chemistry; D051381:Rats; D017207:Rats, Sprague-Dawley; D013482:Superoxide Dismutase / Q000378:metabolism; D017392:Thiobarbituric Acid Reactive Substances / Q000032:analysis
C495626:14-deoxy-11,12-didehydroandrographolide; D000894:Anti-Inflammatory Agents, Non-Steroidal; D004224:Diterpenes; D010936:Plant Extracts; D017392:Thiobarbituric Acid Reactive Substances; C030419:andrographolide; D002374:Catalase; D013482:Superoxide Dismutase; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase