title: [Construction of standard allelic ladder of miniSTR loci by molecular cloning].
abstract: OBJECTIVE
  To investigate the genetic polymorphism of five miniSTR loci (D9S2157, D9S1122, D10S1435, D12ATA63, D2S1776) in Hebei Han population and to construct standard allelic ladders.
  
  
  METHODS
  Polymorphism of the five miniSTR loci in 120 unrelated individuals was analyzed by fluorescence PCR and ABI 310 Genetic Analyzer. Molecular cloning technique was employed to construct standard allelic ladder of the 5 loci.
  
  
  RESULTS
  Of the five miniSTR loci, 8, 8, 7, 5 and 8 alleles were found, respectively. The polymorphism information component were 0.790, 0.720, 0.750, 0.630 and 0.850, respectively.
  
  
  CONCLUSIONS
  The five loci have relatively abundant polymorphic information and their standard allelic ladders constructed by molecular cloning technique are useful in forensic science.
authors.affiliation: Hebei Key Laboratory of Forensic Medicine, Department of Forensic Medicine, Hebei Medical University, Shijiazhuang 050017, China. snowhome2004@126.com
authors.forename: Xue
authors.initials: X
authors.lastname: Bai
authors.forename: Bin
authors.initials: B
authors.lastname: Cong
authors.forename: Shu-Jin
authors.initials: SJ
authors.lastname: Li
authors.forename: Zhi-Ping
authors.initials: ZP
authors.lastname: Hou
authors.forename: Jian-Li
authors.initials: JL
authors.lastname: Gu
authors.forename: Ning
authors.initials: N
authors.lastname: Liu
authors.forename: Xia
authors.initials: X
authors.lastname: Li
authors.forename: Xia
authors.initials: X
authors.lastname: Guo
mesh_terms: D000483:Alleles; D044466:Asian People / Q000235:genetics; D002681:China / Q000208:ethnology; D003001:Cloning, Molecular; D053803:Forensic Genetics; D005787:Gene Frequency; D005828:Genetics, Population; D006801:Humans; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic / Q000235:genetics*; D020080:Tandem Repeat Sequences / Q000235:genetics*