Effect of oxidized fibrinogen on aggregation of activated platelets and neutrophils.
The effect of oxidized fibrinogen on platelet-neutrophil complex formation was evaluated by studying the platelet aggregation (changes in light transmission and turbidimetric assay). Activation of cells by thrombin (0.015 U/ml) in the presence of oxidized fibrinogen was accompanied by the formation of larger intermolecular aggregates of platelets and leukocytes as compared to those detected in experiments with non-oxidized fibrinogen. Addition of thrombin (0.2 U/ml) in the presence of oxidized fibrinogen was followed by the formation of more stable complexes of platelets and leukocytes as compared to those revealed in experiments with non-oxidized fibrinogen. An increase in the width of aggregation curves was most pronounced in the system of 10(-4) M Fe(2+) and 10(-4) M H(2)O(2) with oxidized fibrinogen. Our results indicate that oxidized fibrinogen contributes to the "floating" or suspension of platelet-leukocyte complexes.
Aseychev
A V
AV
Laboratory for Biophysical Bases of Pathology, Institute of Physicochemical Medicine, Federal Agency for Healthcare and Social Development, Moscow, Russia.
Azizova
O A
OA
Shulenina
L V
LV
Piryazev
A P
AP
D005340:Fibrinogen / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology*; D006490:Hemostatics / Q000494:pharmacology; D018375:Neutrophil Activation / Q000187:drug effects / Q000502:physiology*; D009504:Neutrophils / Q000187:drug effects* / Q000502:physiology; D010084:Oxidation-Reduction; D015539:Platelet Activation / Q000187:drug effects / Q000502:physiology*; D010974:Platelet Aggregation / Q000187:drug effects* / Q000502:physiology; D013917:Thrombin / Q000494:pharmacology
D006490:Hemostatics; D005340:Fibrinogen; D013917:Thrombin