Methods for assessing binding of mitochondrial transcription factor A (TFAM) to DNA.
It is now recognized that mammalian mitochondrial DNA forms a higher structure called the nucleoid, corresponding to the nucleosome of nuclear DNA. Mitochondrial transcription factor A (TFAM), which was cloned as a transcription factor for mitochondrial DNA, is essential for the maintenance of mitochondrial DNA. In fact, TFAM markedly enhances the promoter-specific transcription of mitochondrial DNA. In addition, TFAM has an ability to bind to DNA in a sequence-independent manner and is abundant enough to cover an entire region of mitochondrial DNA. Over-expression of human TFAM in cells increases the amount of mitochondrial DNA almost in parallel with the TFAM. TFAM may stabilize mitochondrial DNA by packaging and regulate (or titrate) the amount of mitochondrial DNA. Thus, TFAM may play a crucial role in maintaining mitochondrial DNA as a main component of the nucleoid (or more appropriately mitochromosome).
Fukuoh
Atsushi
A
Department of Clinical Chemistry and Laboratory Medicine, Kyushu University Graduate School of Medical Sciences, Fukuoka, Japan.
Kang
Dongchon
D
D001483:Base Sequence; D047369:Chromatin Immunoprecipitation / Q000379:methods*; D002846:Chromatography, Affinity; D003432:Cross-Linking Reagents; D004261:DNA Replication; D004272:DNA, Mitochondrial / Q000235:genetics / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000032:analysis* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D024202:Electrophoretic Mobility Shift Assay / Q000379:methods*; D006801:Humans; D024101:Mitochondrial Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D011994:Recombinant Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D012689:Sequence Homology, Nucleic Acid; D014157:Transcription Factors / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*
D003432:Cross-Linking Reagents; D004272:DNA, Mitochondrial; D004268:DNA-Binding Proteins; D024101:Mitochondrial Proteins; D011994:Recombinant Proteins; C499631:TFAM protein, human; D014157:Transcription Factors