Insulin-binding canavalin is present in Canavalia ensiformis seed coat.
An insulin-binding protein was isolated from Canavalia ensiformis seed coat, by using an insulin-Sepharose 4B affinity chromatography, and the protein was identified as canavalin (Canavalia 7S globulin) by mass spectrometry analysis. The major novelty of these data is the acidic nature of this globulin insulin-binding, in contrast to the basic Bg-like insulin-binding proteins so far reported in plants.
Ribeiro
Elane da Silva
Eda S
Laboratório de Química e Função de Proteínas e Peptídeos, Centro de Biociôncias e Biotecnologia, Universidade Estadual do Norte Fluminense, 28013-602, Campos dos Goytacazes, RJ, Brazil.
Uchôa
Adriana Ferreira
AF
da Silva
Leonardo Gomes
LG
Beghini
Daniela Gois
DG
Ferreira
Andre Teixeira da Silva
AT
Perales
Jonas
J
Jacinto
Tânia
T
Fernandes
Katia Valevski Sales
KV
Xavier-Filho
José
J
Oliveira
Antonia Elenir Amancio
AE
D032449:Canavalia / Q000737:chemistry*; D002352:Carrier Proteins; D002846:Chromatography, Affinity; D005916:Globulins / Q000737:chemistry; D007328:Insulin / Q000737:chemistry*; D010940:Plant Proteins / Q000737:chemistry*; D012639:Seeds / Q000737:chemistry
D002352:Carrier Proteins; D005916:Globulins; D007328:Insulin; D010940:Plant Proteins; C006882:canavalin protein, Canavalia ensiformis