Resistance of the cell wall to degradation is a critical parameter for isolation of high quality RNA from natural isolates of Bacillus subtilis.
Natural isolates of Bacillus subtilis are known for their ability to produce a large panel of bioactive compounds. Unfortunately, their recalcitrance to conventional molecular techniques limits their transcript studies. In this work, difficulties to isolate RNA attributed to the cell wall were overcome, finally authorising powerful RT-PCR's.
Guez
Jean-Sébastien
JS
Laboratoire ProBioGEM, UPRES-EA 1026, Polytech-Lille, IUT A, Université des Sciences et Technologies de Lille, Bd Paul Langevin, 59655, Villeneuve d'Ascq, France. jean-sebastien.guez@polytech-lille.fr
Coutte
François
F
Drucbert
Anne-Sophie
AS
Chihib
Nour-Eddine
NE
Danzé
Pierre-Marie
PM
Jacques
Philippe
P
D001412:Bacillus subtilis / Q000166:cytology* / Q000235:genetics; D001431:Bacteriological Techniques; D002473:Cell Wall / Q000378:metabolism*; D012329:RNA, Bacterial / Q000302:isolation & purification*; D020133:Reverse Transcriptase Polymerase Chain Reaction
D012329:RNA, Bacterial