Methylation of HACE1 in gastric carcinoma.
BACKGROUND
We recently examined the methylation status of the HACE1 gene in primary carcinomas derived from 32 patients with colorectal cancer. A significant increase was observed in the maximal tumor size of the tumors with methylated HACE1 (p=0.0304). Moreover, a trend was shown toward preferentially developing lymph node metastasis in the carcinomas with methylated HACE1 (p=0.0612), suggesting that HACE1 might present a malignant potential in colorectal cancer. These results prompted us to examine the methylation status of the HACE1 gene in gastric carcinomas.


METHODS
The methylation status of the HACE1 gene was examined in primary carcinomas and the corresponding normal tissues derived from 34 patients with gastric carcinoma using quantitative methylation-specific PCR (qMSP) and the correlation between the methylation status and the clinicopathological findings was evaluated.


RESULTS
An aberrant methylation of the HACE1 gene was detected in 9 out of 34 (26%) primary gastric carcinomas. Subsequently, clinicopathological data were tested for correlation with the methylation score. A significant difference was observed in patient gender (p=0.0429).


CONCLUSIONS
HACE1 was frequently methylated in gastric carcinoma derived from male patients.
Sakata
Makiko
M
Department of Surgery, Showa University Fujigaoka Hospital, Aoba-ku, Yokohama 227-8501, Japan.
Kitamura
Yo-Hei
YH
Sakuraba
Kazuma
K
Goto
Tetsuhiro
T
Mizukami
Hiroki
H
Saito
Mitsuo
M
Ishibashi
Kazuyoshi
K
Kigawa
Gaku
G
Nemoto
Hiroshi
H
Sanada
Yutaka
Y
Hibi
Kenji
K
D000368:Aged; D000369:Aged, 80 and over; D019175:DNA Methylation; D005260:Female; D005753:Gastric Mucosa / Q000378:metabolism / Q000473:pathology; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008207:Lymphatic Metastasis; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D016133:Polymerase Chain Reaction; D011379:Prognosis; D011401:Promoter Regions, Genetic; D013274:Stomach Neoplasms / Q000235:genetics* / Q000473:pathology*; D044767:Ubiquitin-Protein Ligases / Q000235:genetics*
C497506:HACE1 protein, human; D044767:Ubiquitin-Protein Ligases