[Flavonoids extracts from Inula britannica inhibit oxidative stress in vessel after balloon injury].
OBJECTIVE
To observe the inhibition of flavonoids extract (FE) from Inula britannica on oxidative stress in rat aorta after balloon injury.


METHODS
The model of vascular intimal hyperplasia was established by balloon injury. The rats were randomly divided into four groups: control, model, FE and captopril (CAP, positive control). The FE group was treated by intragastric administration with FE in dose of 12.5, 25, 50 mg x kg(-1) x d(-1). The level of malondialdehyde (MDA) and superoxide dismutase (SOD) in serum were detected by thiobarbituric acid (TAB) method and xanthine oxidase method respectively, and superoxide anion (O2-) in vessel was detected by dihydroethidium (DHE) staining. The histochemistry, immunohistochemistry and Western blot were used to observe the changes of appearance and SOD expression in vascular tissues after balloon injury.


RESULTS
The best concentration of FE to rats was 50 mg x kg(-1) x d(-1). The neointima thickness in the model group was significantly higher than that in the FE group (50 mg x kg(-1) x d(-1)) and control group at 14 days after balloon injury (P < 0.01). The lever of MDA in serum of FE group was decreased (P < 0.01) and SOD was increased (P < 0.05) in both serum and vascular tissues. The level of O2*- in the drug group was lower than that in the model group.


CONCLUSIONS
FE can enhance the antioxidation capacities of vessel tissues by suppressing the formation of O2- induced by injury, by which FE inhibites neointima formation after balloon injury in rat.
Zhang
Hongbing
H
Hebei Laboratory of Medical Biotechnology, Institute of Basic Medicine, Heibei Medical University, Shijiazhuang 050017, China.
Han
Mei
M
Wen
Jinkun
J
D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage* / Q000737:chemistry; D001011:Aorta / Q000187:drug effects / Q000378:metabolism; D001808:Blood Vessels / Q000187:drug effects* / Q000378:metabolism; D002404:Catheterization / Q000009:adverse effects*; D015536:Down-Regulation; D004996:Ethidium / Q000031:analogs & derivatives / Q000097:blood / Q000378:metabolism; D005419:Flavonoids / Q000008:administration & dosage* / Q000737:chemistry; D038143:Inula / Q000737:chemistry*; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects*; D011897:Random Allocation; D051381:Rats; D013482:Superoxide Dismutase / Q000097:blood / Q000378:metabolism
D000975:Antioxidants; D005419:Flavonoids; C067883:dihydroethidium; D013482:Superoxide Dismutase; D004996:Ethidium