Antimalarial drug discovery: in silico structural biology and rational drug design.
Malaria remains one of the most burdensome human infectious diseases, with a high rate of resistance outbreaks and a constant need for the discovery of novel antimalarials and drug targets. For several reasons, Plasmodial proteins are difficult to characterise structurally using traditional physical approaches. However, these problems can be partially overcome using a number of in silico approaches. This review describes the peculiarities of malaria proteins and then details various in silico strategies to select and allow descriptions of the molecular structures of drug target candidates as well as subsequent rational approaches for drug design. Chiefly, homology modelling with specific focus on unique aspects of malaria proteins including low homology, large protein size and the presence of parasite-specific inserts is addressed and alternative strategies including multiple sequence and structure-based prediction methods, sampling-based approaches that aim to reveal likely global or shared features of a Plasmodial structure and the value of molecular dynamics understanding of unique features of Plasmodial proteins are discussed. Once a detailed description of the drug target is available, in silico approaches to the specific design of an inhibitory drug thereof becomes invaluable as an economic and rational alternative to chemical library screening.
de Beer
T A P
TA
Bioinformatics and Computational Biology Unit, Department of Biochemistry, School of Biological Sciences, University of Pretoria, Pretoria 0002, South Africa.
Wells
G A
GA
Burger
P B
PB
Joubert
F
F
Marechal
E
E
Birkholtz
L
L
Louw
A I
AI
D000962:Antimalarials / Q000737:chemistry*; D003198:Computer Simulation; D015195:Drug Design; D055808:Drug Discovery; D006801:Humans; D008288:Malaria / Q000188:drug therapy*; D008958:Models, Molecular; D015800:Protozoan Proteins / Q000737:chemistry*; D013329:Structure-Activity Relationship
D000962:Antimalarials; D015800:Protozoan Proteins