The maintenance of specific aspects of neuronal function and behavior is dependent on programmed cell death of adult-generated neurons in the dentate gyrus.
A considerable number of new neurons are generated daily in the dentate gyrus (DG) of the adult hippocampus, but only a subset of these survive, as many adult-generated neurons undergo programmed cell death (PCD). However, the significance of PCD in the adult brain for the functionality of DG circuits is not known. Here, we examined the electrophysiological and behavioral characteristics of Bax-knockout (Bax-KO) mice in which PCD of post-mitotic neurons is prevented. The continuous increase in DG cell numbers in Bax-KO mice resulted in the readjustment of afferent and efferent synaptic connections, represented by age-dependent reductions in the dendritic arborization of DG neurons and in the synaptic contact ratio of mossy fibers with CA3 dendritic spines. These neuroanatomical changes were associated with reductions in synaptic transmission and reduced performance in a contextual fear memory task in 6-month-old Bax-KO mice. These results suggest that the elimination of excess DG neurons via Bax-dependent PCD in the adult brain is required for the normal organization and function of the hippocampus.
Kim
Woon Ryoung
WR
Department of Anatomy, Brain Korea 21, Korea University College of Medicine, Anam-Dong, Seoul, Korea. woongsun@korea.ac.kr
Park
Ok-Hee
OH
Choi
Sukwoo
S
Choi
Se-Young
SY
Park
Soon Kwon
SK
Lee
Kea Joo
KJ
Rhyu
Im Joo
IJ
Kim
Hyun
H
Lee
Yeon Kyung
YK
Kim
Hyun Taek
HT
Oppenheim
Ronald W
RW
Sun
Woong
W
D000818:Animals; D002452:Cell Count; D016923:Cell Death / Q000502:physiology*; D018891:Dentate Gyrus / Q000166:cytology / Q000254:growth & development* / Q000502:physiology*; D019706:Excitatory Postsynaptic Potentials / Q000502:physiology; D005239:Fear; D066298:In Vitro Techniques; D017774:Long-Term Potentiation / Q000502:physiology; D008568:Memory / Q000502:physiology; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D009434:Neural Pathways / Q000166:cytology / Q000254:growth & development / Q000502:physiology; D009474:Neurons / Q000166:cytology / Q000502:physiology*; D013569:Synapses / Q000502:physiology*; D009435:Synaptic Transmission / Q000502:physiology*; D051028:bcl-2-Associated X Protein / Q000235:genetics
C494827:Bax protein, mouse; D051028:bcl-2-Associated X Protein