title: Production of isoflavonoids in callus cultures of Pueraria candollei var. mirifica.
abstract: Pueraria candollei Wall. ex Benth. var. mirifica (Airy Shaw & Suvat.) Niyomdham was investigated for callus induction using Murashige and Skoog (MS) medium containing different plant growth regulators. After 8 weeks of culture, 66-100% of leaf or stem explants formed calli. Calli from stem explants cultured on MS medium supplemented with 0.5 mg/l thidiazuron (TDZ) gave the maximum of shoot induction (16%) and the highest level of total isoflavonoids [(50.39 +/- 7.06) mg/g dry wt], which was 7-fold higher than that of the native tuber [(7.04 +/- 0.29) mg/g dry wt]. These results suggest that addition of TDZ to the culture medium markedly enhances the production of isoflavonoids in calli induced from stem explants of P. candollei var. mirifica.
authors.affiliation: Faculty of Pharmaceutical Sciences, Khon Kaen University, Khon Kaen, 40002, Thailand.
authors.forename: Latiporn
authors.initials: L
authors.lastname: Udomsuk
authors.forename: Kanokwan
authors.initials: K
authors.lastname: Jarukamjorn
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Tanaka
authors.forename: Waraporn
authors.initials: W
authors.lastname: Putalun
mesh_terms: D002478:Cells, Cultured; D005419:Flavonoids / Q000096:biosynthesis*; D010937:Plant Growth Regulators / Q000378:metabolism; D018515:Plant Leaves / Q000378:metabolism; D018520:Plant Shoots / Q000254:growth & development / Q000378:metabolism; D018547:Plant Stems / Q000378:metabolism; D010946:Plants, Medicinal / Q000166:cytology / Q000378:metabolism; D029908:Pueraria / Q000166:cytology / Q000378:metabolism*
chemical_list: D005419:Flavonoids; D010937:Plant Growth Regulators