title: [Simultaneous determination of clozapine, olanzapine and mirtazapine in human plasma by LC-MS/MS].
abstract: OBJECTIVE
  To develop a method for determination of clozapine, olanzapine and mirtazapine in human plasma by liquid chromatography-tandem mass spectrometry(LC-MS/MS).
  
  
  METHODS
  Clozapine, olanzapine and mirtazapine were extracted from plasma samples by using diethyl ether and separated by Agilent Zorbax SB-C18 column(2.1 mm x 150 mm, 5 microm). Electrospray ionization source was applied, positive ion mode was used to detect and multiple reaction monitoring mode was used to quantify clozapine, olanzapine and mirtazapine. Carbamazepine was the internal standard.
  
  
  RESULTS
  The detection limits of clozapine, olanzapine and mirtazapine were within 0.41-0.92 ng/mL. The calibration curve in the concentration range of 10.0-2000.0 ng/mL showed a good linear distribution (r > or = 0.992 4). The average extraction recoveries were within 65.7%-94.2%. Intra-day RSD and inter-day RSD were less than 6% (n = 5).
  
  
  CONCLUSIONS
  This method seems to be quite specific, sensitive and accurate, and can be used to detect clozapine, olanzapine and mirtazapine in forensic and clinical analytic toxicology.
authors.affiliation: Analytical and Testing Center, Wenzhou Medical College, Wenzhou 325035, China. lankywang@163.com
authors.forename: Xian-Qin
authors.initials: XQ
authors.lastname: Wang
authors.forename: Xiao-Jun
authors.initials: XJ
authors.lastname: Pan
authors.forename: Guan-Yang
authors.initials: GY
authors.lastname: Lin
authors.forename: Zheng
authors.initials: Z
authors.lastname: Xiang
authors.forename: Xue-Bao
authors.initials: XB
authors.lastname: Wang
authors.forename: Jian-Zhang
authors.initials: JZ
authors.lastname: Wu
authors.forename: Dan
authors.initials: D
authors.lastname: Lin
mesh_terms: D001569:Benzodiazepines / Q000097:blood*; D002853:Chromatography, Liquid / Q000379:methods; D003024:Clozapine / Q000097:blood*; D053593:Forensic Toxicology; D006801:Humans; D008803:Mianserin / Q000031:analogs & derivatives* / Q000097:blood; D000078785:Mirtazapine; D000077152:Olanzapine; D053719:Tandem Mass Spectrometry / Q000379:methods
chemical_list: D001569:Benzodiazepines; D008803:Mianserin; D000078785:Mirtazapine; D003024:Clozapine; D000077152:Olanzapine