title: Acceptor-photobleaching FRET analysis of core kinetochore and NAC proteins in living human cells.
abstract: Faithful chromatin segregation is mediated and controlled by the kinetochore protein network which assembles at centromeres. In this study, the neighbourhood relations of inner kinetochore and nucleosome-associated complex (NAC) proteins were analysed in living human interphase cells by acceptor photobleaching FRET. The data indicate that CENP-U is in close vicinity to CENP-I as well as to CENP-B and that CENP-M is close to CENP-T.
authors.affiliation: Department of Molecular Biology, Leibniz-Institute for Age Research, Fritz Lipmann Institute, Jena, Germany.
authors.forename: D
authors.initials: D
authors.lastname: Hellwig
authors.forename: C
authors.initials: C
authors.lastname: Hoischen
authors.forename: T
authors.initials: T
authors.lastname: Ulbricht
authors.forename: Stephan
authors.initials: S
authors.lastname: Diekmann
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics; D015153:Blotting, Western; D045744:Cell Line, Tumor; D051936:Centromere Protein B / Q000737:chemistry / Q000378:metabolism; D002868:Chromosomal Proteins, Non-Histone / Q000737:chemistry / Q000378:metabolism; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods; D049452:Green Fluorescent Proteins / Q000235:genetics; D006801:Humans; D007399:Interphase / Q000502:physiology; D018386:Kinetochores / Q000737:chemistry* / Q000378:metabolism; D008164:Luminescent Proteins / Q000235:genetics; D009687:Nuclear Proteins / Q000737:chemistry* / Q000378:metabolism; D038761:Photobleaching; D014162:Transfection
chemical_list: D001426:Bacterial Proteins; C497926:CENPB protein, human; C534001:CENPT protein, human; D051936:Centromere Protein B; D002868:Chromosomal Proteins, Non-Histone; D008164:Luminescent Proteins; D009687:Nuclear Proteins; C060905:centromere protein C; C485184:enhanced green fluorescent protein; C054562:yellow fluorescent protein, Bacteria; D049452:Green Fluorescent Proteins