Oxidoreductase Macrophage Migration Inhibitory Factor is simultaneously increased in leukocyte subsets of patients with severe sepsis.
The oxidoreductase Macrophage Migration Inhibitory Factor (MIF) is discussed as a promising target for immunomodulatory therapy in patients with severe sepsis. Moreover, MIF expresses tautomerase as well as thiol-protein oxidoreductase activities and has a potential role in cellular redox homeostasis, apoptosis inhibition, endotoxin responsiveness as well as regulation of nuclear transcription factors. To further elucidate a potential role of intracellular MIF in severe sepsis, we assessed alterations of intracellular MIF content in peripheral blood leukocytes of patients with severe sepsis in comparison to healthy controls and non-septic patients after major surgery. Intracellular MIF was significantly elevated simultaneously in lymphocytes, B-cells, macrophages and granulocytes of patients with severe sepsis when compared to healthy control individuals (p < 0.05) and increased when compared to non-septic patients after major surgery. In parallel, plasma MIF levels were elevated in severe sepsis (p < 0.05). There was no difference of intracellular MIF in lymphocytes, B-cells, macrophages or granulocytes between surviving and non-surviving patients with severe sepsis (p > 0.05). However, in survivors LPS ex vivo stimulation increased MIF secretion but not in non-survivors of sepsis (p < 0.05). This finding underlines the role of intracellular MIF in inflammatory diseases. It suggests monitoring of intracellular MIF in further clinical and non-clinical research valuable.
Lehmann
Lutz E
LE
Klinik und Poliklinik für Anästhesie und operative Intensivmedizin, Universität Bonn, Bonn, Germany. Lutz.Lehmann@ukb.uni-bonn.de
Weber
Stefan U
SU
Fuchs
Dagmar
D
Book
Malte
M
Klaschik
Sven
S
Schewe
Jens-Christian
JC
Hoeft
Andreas
A
Stüber
Frank
F
D015703:Antigens, CD / Q000097:blood; D018941:Antigens, CD19 / Q000097:blood; D017252:CD3 Complex / Q000097:blood; D015704:CD4 Antigens / Q000097:blood; D016827:CD8 Antigens / Q000097:blood; D015815:Cell Adhesion Molecules / Q000097:blood; D005260:Female; D058851:GPI-Linked Proteins; D006801:Humans; D007962:Leukocytes / Q000201:enzymology*; D018950:Lipopolysaccharide Receptors / Q000097:blood; D016131:Lymphocyte Subsets / Q000201:enzymology; D008263:Macrophage Migration-Inhibitory Factors / Q000097:blood*; D008297:Male; D010088:Oxidoreductases / Q000097:blood*; D018805:Sepsis / Q000097:blood* / Q000201:enzymology / Q000401:mortality
D015703:Antigens, CD; D018941:Antigens, CD19; D017252:CD3 Complex; D015704:CD4 Antigens; D016827:CD8 Antigens; C504120:CEACAM8 protein, human; D015815:Cell Adhesion Molecules; D058851:GPI-Linked Proteins; D018950:Lipopolysaccharide Receptors; D008263:Macrophage Migration-Inhibitory Factors; D010088:Oxidoreductases