Cell protection induced by Acacia salicina extracts: inhibition of genotoxic damage and determination of its antioxidant capacity.
Antioxidant activity of Acacia salicina extracts was determined by the ability of each extract to inhibit lipid peroxidation, to protect against DNA strand scission induced by hydroxyl radicals, and to scavenge the free radical, 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS(*+)). The IC(50) values of the inhibitory activity toward lipid peroxidation of total oligomer flavonoids (TOF), methanol, ethyl acetate, and aqueous extracts were respectively 28, 52, 472, and 480 microg/mL. All extracts have the ability to scavenge the ABTS(*+) radical by a hydrogen-donating mechanism and to protect pKS plasmid DNA against hydroxyl radicals- induced DNA damage. An assay for the ability of A. salicina extracts to prevent mutations induced by various mutagens in Salmonella typhimurium TA102 and TA104 cells was conducted. TOF, methanol, ethyl acetate, and aqueous extracts from leaf parts of A. salicina showed no mutagenicity either with or without the metabolic enzymes preparation (S9). Protection against methylmethanesulfonate-induced mutagenicity was observed for TOF, methanol, and ethyl acetate extracts. Likewise, all extracts exhibited a high inhibition level of the Ames response induced by the indirect mutagen, 2-aminoanthracene. The antigenotoxic activity could be ascribed, at least in part, to their antioxidant properties, but we cannot exclude additionally mechanisms. Thus, A. salicina may serve as an ideal candidate for a cost- effective, readily exploitable natural phytochemical compound.
Bouhlel
Ines
I
Unité de Pharmacognosie/Biologie Moléculaire (99/UR/07-03), Faculté de Pharmacie de Monastir, Monastir, Tunisia.
Bhouri
Wissem
W
Limem
Ilef
I
Boubaker
Jihed
J
Nefatti
Aicha
A
Skandrani
Ines
I
Ben Sghaier
Mohammed
M
Kilani
Soumaya
S
Ghedira
Kamel
K
Chekir Ghedira
Leila
L
D000045:Acacia / Q000737:chemistry*; D016587:Antimutagenic Agents / Q000494:pharmacology*; D000975:Antioxidants / Q000494:pharmacology*; D045744:Cell Line, Tumor; D019610:Cytoprotection / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D005419:Flavonoids / Q000494:pharmacology; D016166:Free Radical Scavengers; D006801:Humans; D020014:K562 Cells; D015227:Lipid Peroxidation / Q000187:drug effects*; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology*; D020011:Protective Agents / Q000494:pharmacology
D016587:Antimutagenic Agents; D000975:Antioxidants; D005419:Flavonoids; D016166:Free Radical Scavengers; D010936:Plant Extracts; D020011:Protective Agents