Targeting and internalization of sterically stabilized liposome modified with ZCH-4-2E8.
Sterically stabilized liposome (SL) modified with 2E8 monoclonal antibody (2E8-SL) was prepared in order to evaluate its targeting ability and internalization efficiency against tumor cells with high expression of CD19. 2E8 was coupled to the surface of SL using post-insertion technique. The shape of liposomes was observed under a transmission electronic microscope. The average size of liposomes was determined by using the Zetasizer instrument. The binding and internalization of 2E8-SL against tumor cells with higher expression of CD19 were tested by flow cytometry and confocal microscopy. The mean diameter of 2E8-SL was 121.25 nm. 2E8-SL was stable after up to 24 days in various buffers. 2E8-SL showed specific binding to tumor cells with high expression of CD19, including B cells in peripheral blood mononuclear cells (PBMCs). 2E8-SL could efficiently internalize into Nalm6 cells with CD19 high expression. It is suggested that 2E8-SL may serve as useful delivery carrier of anti-cancer drugs targeting to hematological malignant tumors with CD19 high expression.
Zhang
Jingying
J
Department of Hematology-Oncology, Children' Hospital, Zhejiang University School of Medicine, Hangzhou, 310003, China. zhangjy423@126.com
Tang
Yongmin
Y
Shen
Hongqiang
H
Qian
Baiqin
B
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000276:immunology*; D000912:Antibodies, Neoplasm / Q000032:analysis / Q000276:immunology; D018941:Antigens, CD19 / Q000096:biosynthesis / Q000276:immunology*; D000970:Antineoplastic Agents / Q000008:administration & dosage; D001402:B-Lymphocytes / Q000166:cytology / Q000276:immunology; D045744:Cell Line, Tumor; D004337:Drug Carriers; D016503:Drug Delivery Systems; D004355:Drug Stability; D005260:Female; D006801:Humans; D007140:Immunoglobulin Fab Fragments / Q000276:immunology; D020014:K562 Cells; D008081:Liposomes / Q000737:chemistry / Q000276:immunology / Q000378:metabolism; D016393:Lymphoma, B-Cell / Q000473:pathology; D051379:Mice; D008807:Mice, Inbred BALB C; D011092:Polyethylene Glycols / Q000737:chemistry
D000911:Antibodies, Monoclonal; D000912:Antibodies, Neoplasm; D018941:Antigens, CD19; D000970:Antineoplastic Agents; D004337:Drug Carriers; D007140:Immunoglobulin Fab Fragments; D008081:Liposomes; D011092:Polyethylene Glycols