The expression analysis of periostin in human breast cancer.
BACKGROUND
To investigate the expression of periostin in breast cancer and its clinical relevance.


METHODS
Periostin mRNA expression in breast tissues was measured by RT-PCR. Periostin protein in breast tissues was evaluated by Western blot. Immunohistochemistry method (Envision method) was carried out to detect the expression of periostin in breast cancer tissues of different clinical stages and metastasized lymph nodes, as well as benign breast disease.


RESULTS
The expression of periostin in cancer tissue was increased at both mRNA level (P < 0.05) and protein level (P < 0.05) compared with benign and normal breast tissues. The periostin protein expression level increased with breast cancer clinical stages as shown by IHC.


CONCLUSIONS
Periostin was overexpressed in breast cancer tissues as opposed to normal breast, which implies its role in the pathogenesis and development of this disorder.
Zhang
Yanan
Y
Department of General Surgery, Affiliated Zhong-Da Hospital, Southeast University, Nanjing, China. zhangyanan79@yahoo.com.cn
Zhang
Guangyuan
G
Li
Junsheng
J
Tao
Qingsong
Q
Tang
Wenhao
W
D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000378:metabolism*; D001943:Breast Neoplasms / Q000378:metabolism*; D018270:Carcinoma, Ductal, Breast / Q000378:metabolism*; D015815:Cell Adhesion Molecules / Q000378:metabolism*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007150:Immunohistochemistry; D008875:Middle Aged; D012333:RNA, Messenger / Q000378:metabolism; D015854:Up-Regulation
D014408:Biomarkers, Tumor; D015815:Cell Adhesion Molecules; C512835:POSTN protein, human; D012333:RNA, Messenger