title: Genome-wide RNAi screen identifies genes involved in intestinal pathogenic bacterial infection.
abstract: Innate immunity represents the first line of defense in animals. We report a genome-wide in vivo Drosophila RNA interference screen to uncover genes involved in susceptibility or resistance to intestinal infection with the bacterium Serratia marcescens. We first employed whole-organism gene suppression, followed by tissue-specific silencing in gut epithelium or hemocytes to identify several hundred genes involved in intestinal antibacterial immunity. Among the pathways identified, we showed that the JAK-STAT signaling pathway controls host defense in the gut by regulating stem cell proliferation and thus epithelial cell homeostasis. Therefore, we revealed multiple genes involved in antibacterial defense and the regulation of innate immunity.
authors.affiliation: IMBA, Institute of Molecular Biotechnology of the Austrian Academy of Sciences, A-1030 Vienna, Austria.
authors.forename: Shane J F
authors.initials: SJ
authors.lastname: Cronin
authors.forename: Nadine T
authors.initials: NT
authors.lastname: Nehme
authors.forename: Stefanie
authors.initials: S
authors.lastname: Limmer
authors.forename: Samuel
authors.initials: S
authors.lastname: Liegeois
authors.forename: J Andrew
authors.initials: JA
authors.lastname: Pospisilik
authors.forename: Daniel
authors.initials: D
authors.lastname: Schramek
authors.forename: Andreas
authors.initials: A
authors.lastname: Leibbrandt
authors.forename: Ricardo de Matos
authors.initials: Rde M
authors.lastname: Simoes
authors.forename: Susanne
authors.initials: S
authors.lastname: Gruber
authors.forename: Urszula
authors.initials: U
authors.lastname: Puc
authors.forename: Ingo
authors.initials: I
authors.lastname: Ebersberger
authors.forename: Tamara
authors.initials: T
authors.lastname: Zoranovic
authors.forename: G Gregory
authors.initials: GG
authors.lastname: Neely
authors.forename: Arndt
authors.initials: A
authors.lastname: von Haeseler
authors.forename: Dominique
authors.initials: D
authors.lastname: Ferrandon
authors.forename: Josef M
authors.initials: JM
authors.lastname: Penninger
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D049109:Cell Proliferation; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism; D004331:Drosophila melanogaster / Q000235:genetics* / Q000276:immunology / Q000382:microbiology*; D004847:Epithelial Cells / Q000166:cytology / Q000502:physiology; D049750:Genome, Insect; D006434:Hemocytes / Q000276:immunology / Q000378:metabolism / Q000382:microbiology; D006706:Homeostasis; D007113:Immunity, Innate / Q000235:genetics*; D007413:Intestinal Mucosa / Q000166:cytology / Q000276:immunology / Q000378:metabolism / Q000382:microbiology; D053612:Janus Kinases / Q000235:genetics / Q000378:metabolism; D023421:Models, Animal; D034622:RNA Interference; D050791:STAT Transcription Factors / Q000235:genetics / Q000378:metabolism; D016868:Serratia Infections / Q000235:genetics / Q000276:immunology* / Q000382:microbiology; D012706:Serratia marcescens / Q000276:immunology* / Q000502:physiology; D015398:Signal Transduction; D013234:Stem Cells / Q000166:cytology / Q000502:physiology
chemical_list: D029721:Drosophila Proteins; D050791:STAT Transcription Factors; D053612:Janus Kinases