Characterisation of mixed lithium dodecyl sulphate/lithium perfluorooctanesulphonate pseudo-stationary phases in MEKC.
Lipophilicity and methylene selectivity of mixed pseudo-stationary phases (PSPs) (containing lithium dodecyl sulphate (LDS) and lithium perfluorooctanesulphonate (LiPFOS) in different molar ratios) applied in MEKC have been investigated. Micellar proportion (t(prop,mic), a quantity expressing that how much time is spent by the analyte in the micellar phase related to its whole migration time), CLOGP(50) value (showing the value of hydrophobicity of a molecule spending exactly 50% of its migration time in the PSP) and methylene selectivity have been determined for different LDS/LiPFOS mixed phases. Values of the above-mentioned parameters have been determined for analytes with different chemical structures (alkylbenzene and alkylphenone homologous series, alcohols). Good linear correlation was obtained between either the micellar proportion, CLOGP(50), or methylene selectivity and the phase composition for the mixed phases. Lipophilicity and methylene selectivity of the mixed LDS/LiPFOS PSPs can be calculated and can continuously be changed by mixing the two single phases (LDS and LiPFOS) in the appropriate (and calculable) portion.
Varga
András
A
Pathobiochemistry Research Group of Hungarian Academy of Sciences and Semmelweis University, Hungary. andras.varga@kkk.org.hu
Huszár
Mónika
M
Dobos
Zsófia
Z
Kiss
Eva
E
Horváth
Anikó
A
Idei
Miklós
M
D017738:Alkanesulfonic Acids / Q000737:chemistry*; D020374:Chromatography, Micellar Electrokinetic Capillary / Q000379:methods*; D005466:Fluorocarbons / Q000737:chemistry*; D006838:Hydrocarbons / Q000737:chemistry; D057927:Hydrophobic and Hydrophilic Interactions; D016014:Linear Models; D018020:Lithium Compounds / Q000737:chemistry*; D008823:Micelles; D008956:Models, Chemical; D012680:Sensitivity and Specificity; D012967:Sodium Dodecyl Sulfate / Q000737:chemistry*
D017738:Alkanesulfonic Acids; D005466:Fluorocarbons; D006838:Hydrocarbons; D018020:Lithium Compounds; D008823:Micelles; D012967:Sodium Dodecyl Sulfate; C076994:perfluorooctane sulfonic acid; C028913:dodecyl sulfate