title: No association of the NFKB1 insertion/deletion promoter polymorphism with survival in patients with gastric cancer.
abstract: OBJECTIVE
  The present study analyzed the functional insertion/deletion polymorphism in the promoter region of NFKB1 gene and their impact on the prognosis for patients with gastric adenocarcinoma.
  
  
  METHODS
  Four hundred and seventy two consecutive patients with curatively resected gastric adenocarcinoma were enrolled in the present study. The genomic DNA was extracted from paraffin-embedded tissue and the -94 insertion/deletion ATTG polymorphism of NFKB1 determined using a polymerase chain reaction-restriction fragment length polymorphism assay.
  
  
  RESULTS
  The NFKB1 promoter gene polymorphism was successfully amplified in 97.8% of the cases. There were no sexual differences in relation to the genotype and allele. No correlation was observed between the frequency of the genotype or allele and the T, N or M stage. The multivariate survival analysis showed no association between the NFKB1 -94 insertion/deletion promoter polymorphism and the disease-free survival or overall survival of the patients with gastric cancer.
  
  
  CONCLUSIONS
  The functional NFKB1 promoter polymorphism was not found to be a prognostic marker for Korean patients with surgically resected gastric adenocarcinoma.
authors.affiliation: Department of Surgery, Kyungpook National University Hospital, Kyungpook National University School of Medicine, 50 Samduck 2-Ga, Jung-Gu, Daegu 700-712, Korea. wyu@mail.knu.ac.kr
authors.forename: Jong Gwang
authors.initials: JG
authors.lastname: Kim
authors.forename: Sang Kyun
authors.initials: SK
authors.lastname: Sohn
authors.forename: Yee Soo
authors.initials: YS
authors.lastname: Chae
authors.forename: Joon Ho
authors.initials: JH
authors.lastname: Moon
authors.forename: Shi Nae
authors.initials: SN
authors.lastname: Kim
authors.forename: Byung Woog
authors.initials: BW
authors.lastname: Kang
authors.forename: Gab Chul
authors.initials: GC
authors.lastname: Kim
authors.forename: Myung-Hoon
authors.initials: MH
authors.lastname: Lee
authors.forename: Seong Woo
authors.initials: SW
authors.lastname: Jeon
authors.forename: Ho Young
authors.initials: HY
authors.lastname: Chung
authors.forename: Wansik
authors.initials: W
authors.lastname: Yu
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics / Q000401:mortality / Q000556:secondary; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D004273:DNA, Neoplasm / Q000235:genetics / Q000378:metabolism; D005240:Feasibility Studies; D005260:Female; D017353:Gene Deletion; D006801:Humans; D007723:Korea; D008297:Male; D008875:Middle Aged; D016254:Mutagenesis, Insertional; D052002:NF-kappa B p50 Subunit / Q000235:genetics*; D009367:Neoplasm Staging; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D011379:Prognosis; D011401:Promoter Regions, Genetic / Q000235:genetics*; D013274:Stomach Neoplasms / Q000235:genetics* / Q000401:mortality* / Q000473:pathology; D015996:Survival Rate; D016896:Treatment Outcome
chemical_list: D004273:DNA, Neoplasm; D052002:NF-kappa B p50 Subunit; C489548:NFKB1 protein, human