Synthesis of Lewis X epitopes on plant N-glycans.
Glycoproteins from tobacco line xFxG1, in which expression of a hybrid beta-(1-->4)-galactosyltransferase (GalT) and a hybrid alpha-(1-->3)-fucosyltransferase IXa (FUT9a) is combined, contained an abundance of hybrid N-glycans with Lewis X (Le(X)) epitopes. A comparison with N-glycan profiles from plants expressing only the hybrid beta-(1-->4)-galactosyltransferase suggested that the fucosylation of the LacNAc residues in line xFxG1 protected galactosylated N-glycans from endogenous plant beta-galactosidase activity.
Rouwendal
Gerard J A
GJ
Plant Research International B.V., Wageningen University and Research Center, Droevendaalsesteeg 1, 6708 PB Wageningen, The Netherlands. gerard.rouwendal@synthon.nl
Florack
Dion E A
DE
Hesselink
Thamara
T
Cordewener
Jan H
JH
Helsper
Johannes P F G
JP
Bosch
Dirk
D
D000818:Animals; D000939:Epitopes / Q000096:biosynthesis* / Q000276:immunology; D005647:Fucosyltransferases / Q000235:genetics / Q000378:metabolism; D005700:Galactosyltransferases / Q000235:genetics / Q000378:metabolism; D006801:Humans; D030821:Plants, Genetically Modified / Q000235:genetics / Q000378:metabolism*; D011134:Polysaccharides / Q000096:biosynthesis* / Q000276:immunology; D021241:Spectrometry, Mass, Electrospray Ionization; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D053719:Tandem Mass Spectrometry; D014026:Nicotiana / Q000235:genetics / Q000378:metabolism*
D000939:Epitopes; D011134:Polysaccharides; D005647:Fucosyltransferases; D005700:Galactosyltransferases