Measurement of sulfur-containing compounds involved in the metabolism and transport of cysteamine and cystamine. Regional differences in cerebral metabolism.
An HPLC method with coulometric detection is presented for the quantitation of cysteamine, cystamine, thialysine, glutathione, glutathione disulfide and an oxidized metabolite of thialysine [S-(2-aminoethyl)-L-cysteine ketimine decarboxylated dimer (AECK-DD)]. The advantage of coulometric detection is that derivatization is unnecessary if the analyte is redox sensitive. The method was used to quantitate several sulfur-containing compounds in plasma and brain following gavage feeding of cysteamine to rats. Cysteamine, cystamine, thialysine and AECK-DD were detected in the brains of these animals. Interestingly, cysteamine treatment resulted in greatly elevated levels of cerebral methionine, despite the fact that cysteamine is not a precursor of methionine.
Pinto
John T
JT
Department of Biochemistry and Molecular Biology, New York Medical College, Valhalla, NY 10595, USA.
Khomenko
Tetyana
T
Szabo
Sandor
S
McLaren
Gordon D
GD
Denton
Travis T
TT
Krasnikov
Boris F
BF
Jeitner
Thomas M
TM
Cooper
Arthur J L
AJ
D000818:Animals; D001692:Biological Transport; D054022:Cerebrum / Q000737:chemistry / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D003538:Cystamine / Q000378:metabolism*; D003543:Cysteamine / Q000378:metabolism*; D008297:Male; D010084:Oxidation-Reduction; D051381:Rats; D017207:Rats, Sprague-Dawley; D013457:Sulfur Compounds / Q000032:analysis* / Q000097:blood / Q000378:metabolism
D013457:Sulfur Compounds; D003543:Cysteamine; D003538:Cystamine