A proteomic study of pectin-degrading enzymes secreted by Botrytis cinerea grown in liquid culture.
Botrytis cinerea is a pathogenic filamentous fungus, which infects more than 200 plant species. The enzymes secreted by B. cinerea play an important role in the successful colonization of a host plant. Some of the secreted enzymes are involved in the degradation of pectin, a major component of the plant cell wall. A total of 126 proteins secreted by B. cinerea were identified by growing the fungus on highly or partially esterified pectin, or on sucrose in liquid culture. Sixty-seven common proteins were identified in each of the growth conditions, of which 50 proteins exhibited a SignalP motif. Thirteen B. cinerea proteins with functions related to pectin degradation were identified in both pectin growth conditions, while only four were identified in sucrose. Our results indicate it is unlikely that the activation of B. cinerea from the dormant state to active infection is solely dependent on changes in the degree of esterification of the pectin component of the plant cell wall. Further, these results suggest that future studies of the B. cinerea secretome in infections of ripe and unripe fruits will provide important information that will describe the mechanisms that the fungus employs to access nutrients and decompose tissues.
Shah
Punit
P
Complex Carbohydrate Research Center, University of Georgia, Athens, GA 30602-4712, USA.
Gutierrez-Sanchez
Gerardo
G
Orlando
Ron
R
Bergmann
Carl
C
D020171:Botrytis / Q000201:enzymology*; D018929:Cell Culture Techniques; D004951:Esterification; D005638:Fruit / Q000378:metabolism; D005656:Fungal Proteins / Q000032:analysis / Q000378:metabolism*; D010368:Pectins / Q000378:metabolism*; D010446:Peptide Fragments / Q000032:analysis / Q000378:metabolism; D011134:Polysaccharides / Q000378:metabolism; D020543:Proteome / Q000378:metabolism*; D040901:Proteomics / Q000379:methods*; D013395:Sucrose / Q000378:metabolism; D053719:Tandem Mass Spectrometry / Q000379:methods
D005656:Fungal Proteins; D010446:Peptide Fragments; D011134:Polysaccharides; D020543:Proteome; D013395:Sucrose; D010368:Pectins