The tight junction protein ZO-2 blocks cell cycle progression and inhibits cyclin D1 expression.
ZO-2 is an adaptor protein of the tight junction that belongs to the MAGUK protein family. ZO-2 is a dual localization protein that in sparse cultures is present at the cell borders and the nuclei, whereas in confluent cultures it is concentrated at the cell boundaries. Here we have studied whether ZO-2 is able to regulate the expression of cyclin D1 (CD1) and cell proliferation. We have demonstrated that ZO-2 negatively regulates CD1 transcription by interacting with c-Myc at an E box present in CD1 promoter. We have further found that ZO-2 transfection into epithelial MDCK cells triggers a diminished expression of CD1 protein and decreases the rate of cell proliferation in a wound-healing assay.
Gonzalez-Mariscal
Lorenza
L
Department of Physiology, Center for Research and Advanced Studies (CINVESTAV), Mexico City, Mexico. lorenza@fisio.cinvestav.mx
Tapia
Rocio
R
Huerta
Miriam
M
Lopez-Bayghen
Esther
E
D000818:Animals; D002453:Cell Cycle; D002478:Cells, Cultured; D019938:Cyclin D1 / Q000235:genetics* / Q000378:metabolism; D004285:Dogs; D015536:Down-Regulation; D015870:Gene Expression; D020570:Genes, myb; D006801:Humans; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D010750:Phosphoproteins / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D019108:Tight Junctions / Q000378:metabolism*; D014158:Transcription, Genetic; D014162:Transfection; D062826:Zonula Occludens-1 Protein; D062827:Zonula Occludens-2 Protein
D008565:Membrane Proteins; D010750:Phosphoproteins; D012333:RNA, Messenger; C569042:TJP1 protein, human; C569046:TJP2 protein, human; D062826:Zonula Occludens-1 Protein; D062827:Zonula Occludens-2 Protein; D019938:Cyclin D1