Biotransformation of ginsenoside Rf to Rh1 by recombinant beta-glucosidase.
An Aspergillus niger strain was isolated from the soil around ginseng fruit. In vitro enzyme assays showed that this strain had the ability to transform total ginsenosides (TGS) into several new products. In a further biochemical study, a beta-glucosidase gene isolated from this strain, bgl1, was expressed in Saccharomyces cerevisiae. His-tagged BGL1 protein (approximately 170 kD) showed the ability to transform ginsenoside Rf into Rh(1).
Ruan
Chang-Chun
CC
College of Chinese Medicinal Materials, Jilin Agricultural University, Changchun, 130118, China.
Zhang
Hao
H
Zhang
Lian-Xue
LX
Liu
Zhi
Z
Sun
Guang-Zhi
GZ
Lei
Jun
J
Qin
Yu-Xia
YX
Zheng
Yi-Nan
YN
Li
Xiang
X
Pan
Hong-Yu
HY
D001234:Aspergillus niger / Q000201:enzymology; D002851:Chromatography, High Pressure Liquid; D036145:Ginsenosides / Q000737:chemistry / Q000378:metabolism*; D015394:Molecular Structure; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism; D001617:beta-Glucosidase / Q000235:genetics / Q000378:metabolism*
D036145:Ginsenosides; D011994:Recombinant Proteins; C425564:ginsenoside Rh1; D001617:beta-Glucosidase; C055328:ginsenoside Rf