Direct electron transfer of glucose oxidase immobilized in an ionic liquid reconstituted cellulose-carbon nanotube matrix.
Conductive cellulose-multiwalled carbon nanotube (MWCNT) matrix with a porous structure and good biocompatibility has been prepared using a room temperature ionic liquid (1-ethyl-3-methylimidazolium acetate) as solvent. Glucose oxidase (GOx) was encapsulated in this matrix and thereby immobilized on a glassy carbon surface. The direct electron transfer and electrocatalysis of the encapsulated GOx has been investigated using cyclic voltammetry and chronoamperometry. The GOx exhibited a pair of stable, well defined and nearly symmetric reversible redox peaks. The experimental results also demonstrate that the immobilized GOx retains its biocatalytic activity toward the oxidation of glucose and therefore can be employed in a glucose biosensor. The results show that the bioelectrode modified by the cellulose-MWCNT matrix has potential for use in biosensors and other bioelectronics devices.
Wu
Xuee
X
Chemical Sciences, University of Surrey, Guildford, GU2 7XH, United Kingdom. Xuee.wu@surrey.ac.uk
Zhao
Feng
F
Varcoe
John R
JR
Thumser
Alfred E
AE
Avignone-Rossa
Claudio
C
Slade
Robert C T
RC
D001234:Aspergillus niger / Q000201:enzymology; D055162:Biocatalysis; D015374:Biosensing Techniques; D020134:Catalytic Domain; D002482:Cellulose / Q000737:chemistry*; D004563:Electrochemistry; D004579:Electron Transport; D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000737:chemistry* / Q000378:metabolism*; D005949:Glucose Oxidase / Q000737:chemistry* / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007093:Imidazoles / Q000737:chemistry; D052578:Ionic Liquids / Q000737:chemistry*; D037742:Nanotubes, Carbon / Q000737:chemistry*; D015203:Reproducibility of Results; D012996:Solutions; D012997:Solvents / Q000737:chemistry
D004800:Enzymes, Immobilized; D007093:Imidazoles; D052578:Ionic Liquids; D037742:Nanotubes, Carbon; D012996:Solutions; D012997:Solvents; C502841:1-butyl-3-methylimidazolium chloride; D002482:Cellulose; D005949:Glucose Oxidase; C518739:1-ethyl-3-methylimidazolium