title: Diversity of bacteria and fungi in aerosols during screening in a green waste composting plant.
abstract: This article outlines a comprehensive analysis of the microbial diversity of aerosols produced during screening in a green waste composting plant using both culture and molecular techniques. Bacteria, thermophilic actinomycetes and fungi were quantified in the aerosols. The structure of the microbial community was examined using a fingerprint technique and DNA libraries. The results show: (i) the very high diversity of bacteria and fungi in aerosols produced during the composting screening stage, (ii) the low percentage of cultivability for bacteria in aerosols, (iii) the abundance of Thermoactinomyces spp. and Aspergillus spp. in compost aerosols.
authors.affiliation: Laboratoire de Biotechnologie de l'Environnement, INRA, UR50, Avenue des Etangs, Narbonne, 11100, France.
authors.forename: Valérie
authors.initials: V
authors.lastname: Bru-Adan
authors.forename: Nathalie
authors.initials: N
authors.lastname: Wéry
authors.forename: Marina
authors.initials: M
authors.lastname: Moletta-Denat
authors.forename: Patrick
authors.initials: P
authors.lastname: Boiron
authors.forename: Jean-Philippe
authors.initials: JP
authors.lastname: Delgènes
authors.forename: Jean-Jacques
authors.initials: JJ
authors.lastname: Godon
mesh_terms: D000336:Aerosols; D001419:Bacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D044822:Biodiversity; D016000:Cluster Analysis; D015169:Colony Count, Microbial; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005658:Fungi / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D020459:Genes, rRNA; D008969:Molecular Sequence Data; D010802:Phylogeny; D012329:RNA, Bacterial / Q000235:genetics; D012331:RNA, Fungal / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012337:RNA, Ribosomal, 18S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D012987:Soil; D012988:Soil Microbiology; D018505:Waste Management / Q000379:methods
chemical_list: D000336:Aerosols; D004269:DNA, Bacterial; D004271:DNA, Fungal; D004275:DNA, Ribosomal; D012329:RNA, Bacterial; D012331:RNA, Fungal; D012336:RNA, Ribosomal, 16S; D012337:RNA, Ribosomal, 18S; D012987:Soil