Electrochemical sensor for multiplex biomarkers detection.
OBJECTIVE
Multiplexing assay of biomarkers at the point-of-care is an elusive goal for molecular diagnostics.


METHODS
Here, we report an electrochemical (EC) sensor for oral cancer detection based on the simultaneous detection of two salivary biomarkers: interleukin (IL)-8 mRNA and IL-8 protein.


RESULTS
Under the multiplexing mode, the limit of detection of salivary IL-8 mRNA reaches to 3.9 fM and 7.4 pg/mL for IL-8 protein in saliva. Multiplex assay of these 2 biomarkers directly from 28 cancer and 28 matched control saliva samples shows significant difference between the two groups. From the receiver operating characteristic analysis, the EC sensor yields around 90% sensitivity and specificity for both IL-8 mRNA and IL-8 protein, which are very close to the data measured by traditional assays (ELISA and PCR) with the same group of saliva. Combined IL-8 mRNA and protein show better AUC compared with single biomarker.


CONCLUSIONS
We show, for the first time, concurrently multiplexing detection of salivary mRNA and protein biomarkers using point-of-care EC sensor.
Wei
Fang
F
Department of Mechanical and Aerospace Engineering, University of California, Los Angeles, California, USA.
Patel
Prabhudas
P
Liao
Wei
W
Chaudhry
Kishore
K
Zhang
Lei
L
Arellano-Garcia
Martha
M
Hu
Shen
S
Elashoff
David
D
Zhou
Hui
H
Shukla
Shilin
S
Shah
Franky
F
Ho
Chih-Ming
CM
Wong
David T
DT
D014408:Biomarkers, Tumor / Q000032:analysis* / Q000235:genetics / Q000378:metabolism; D015374:Biosensing Techniques / Q000379:methods*; D016022:Case-Control Studies; D055088:Early Detection of Cancer; D055664:Electrochemical Techniques; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D006801:Humans; D020382:Interleukin-18 / Q000032:analysis / Q000235:genetics / Q000378:metabolism; D008954:Models, Biological; D009062:Mouth Neoplasms / Q000175:diagnosis* / Q000235:genetics / Q000378:metabolism; D016133:Polymerase Chain Reaction / Q000379:methods; D012333:RNA, Messenger / Q000032:analysis; D012372:ROC Curve; D012463:Saliva / Q000737:chemistry / Q000378:metabolism; D012680:Sensitivity and Specificity
D014408:Biomarkers, Tumor; D020382:Interleukin-18; D012333:RNA, Messenger