title: A generally applicable assay for the quantification of inhibitory effects on PCR.
abstract: PCR amplification of target genes from environmental DNA extracts can suffer from PCR inhibition, caused by co-extracted substances. No simple assay has been available to quantify this inhibition. Therefore, a generally applicable PCR inhibition-assay was developed, which allows determination of statistically significant inhibition of PCR. This information is important when documenting quality of DNA in environmental extracts used as template for PCR.
authors.affiliation: Molecular Ecology, Agroscope Reckenholz-Tänikon Research Station ART, Reckenholzstrasse 191, 8046 Zürich, Switzerland.
authors.forename: Salome
authors.initials: S
authors.lastname: Schneider
authors.forename: Jürg
authors.initials: J
authors.lastname: Enkerli
authors.forename: Franco
authors.initials: F
authors.lastname: Widmer
mesh_terms: D004783:Environmental Microbiology; D004791:Enzyme Inhibitors / Q000032:analysis*; D019384:Nucleic Acid Synthesis Inhibitors; D016133:Polymerase Chain Reaction / Q000379:methods* / Q000592:standards*
chemical_list: D004791:Enzyme Inhibitors; D019384:Nucleic Acid Synthesis Inhibitors