Simultaneous RP-HPLC determination of sotalol, metoprolol, alpha-hydroxymetoprolol, paracetamol and its glucuronide and sulfate metabolites in human urine.
The HPLC method for the determination of sotalol (SOT), metoprolol (MET) and alpha-hydroxymetoprolol metabolite (MET-H), paracetamol (PAR), paracetamol glucuronide (PAR-G) and paracetamol sulfate (PAR-S) in human urine is described. Analyses were carried out on a reversed-phase LiChroCART Purospher C18e column (125 mm x 3 mm, 5 microm particles) (Merck) with gradient elution as well as spectrophotometric and fluorometric detection. Good resolution of the analyzed substances was obtained within a time range of no longer than 15 min. The linearity ranges of the callibration curves in human urine (as matrix) were: 3.25-45 microg ml(-1) (SOT), 0.75-40 microg ml(-1) (MET), 0.6-40 microg ml(-1) (MET-H), 4.6-60 microg ml(-1) (PAR-G), 4.95-50 microg ml(-1) (PAR-S), 1.95-45 microg ml(-1) (PAR). An application to human urine samples was performed.
Baranowska
Irena
I
Department of Analytical and General Chemistry, Chemical Faculty, Silesian University of Technology, Strzody 7, 44-100 Gliwice, Poland. irena.baranowska@polsl.pl
Wilczek
Andrzej
A
D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D020719:Glucuronides / Q000378:metabolism / Q000652:urine*; D006801:Humans; D008790:Metoprolol / Q000031:analogs & derivatives* / Q000652:urine*; D015394:Molecular Structure; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013015:Sotalol / Q000652:urine*; D013431:Sulfates / Q000378:metabolism / Q000652:urine*
D020719:Glucuronides; D013431:Sulfates; C029504:alpha-hydroxymetoprolol; D013015:Sotalol; D008790:Metoprolol