title: Development of an industrial medium for economical 2,3-butanediol production through co-fermentation of glucose and xylose by Klebsiella oxytoca.
abstract: An industrial medium containing urea as a sole nitrogen source, low levels of corn steep liquor and mineral salts as nutrition factors to retain high 2,3-butanediol production through co-fermentation of glucose and xylose (2:1, wt/wt) by Klebsiella oxytoca was developed. Urea and corn steep liquor were identified as the most significant factors by the two-level Plackett-Burman design. Steepest ascent experiments were applied to approach the optimal region of the two factors and a central composite design was employed to determine their optimal levels. Under the optimal medium, the yield of 2,3-butanediol plus acetoin relative to glucose and xylose was up to 0.428 g/g, which was 85.6% of theoretical value. The cheap nitrogen source and nutrition factors combining the co-fermentation process using lignocellulose derived glucose and xylose as the carbon source in the developed medium would be a potential solution to improve the economics of microbial 2,3-butanediol production.
authors.affiliation: State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, No. 5 Xinmofan Road, Nanjing 210009, People's Republic of China.
authors.forename: Xiao-Jun
authors.initials: XJ
authors.lastname: Ji
authors.forename: He
authors.initials: H
authors.lastname: Huang
authors.forename: Jun
authors.initials: J
authors.lastname: Du
authors.forename: Jian-Guo
authors.initials: JG
authors.lastname: Zhu
authors.forename: Lu-Jing
authors.initials: LJ
authors.lastname: Ren
authors.forename: Shuang
authors.initials: S
authors.lastname: Li
authors.forename: Zhi-Kui
authors.initials: ZK
authors.lastname: Nie
mesh_terms: D002072:Butylene Glycols / Q000378:metabolism*; D003470:Culture Media / Q000138:chemical synthesis*; D005285:Fermentation; D005947:Glucose / Q000378:metabolism*; D007221:Industry; D041121:Klebsiella oxytoca / Q000378:metabolism*; D008954:Models, Biological; D012044:Regression Analysis; D015203:Reproducibility of Results; D013499:Surface Properties; D013997:Time Factors; D014994:Xylose / Q000378:metabolism*
chemical_list: D002072:Butylene Glycols; D003470:Culture Media; C026978:2,3-butylene glycol; D014994:Xylose; D005947:Glucose