title: Nitric oxide in the solitary tract nucleus (STn) modulates glucose homeostasis and FOS-ir expression after carotid chemoreceptor stimulation.
abstract: We evaluate in rats the role of NO in the solitary tract nucleus (STn) after an anoxic stimulus to carotid body chemoreceptor cells (CChrc) with cyanide (NaCN), on the hyperglycemic reflex with glucose retention by the brain (BGR) and FOS expression (FOS-ir) in the STn. The results suggest that nitroxidergic pathways in the STn may play an important role in glucose homeostasis. A NO donor such as sodium nitroprusside (NPS) in the STn before CChrc stimulation increased arterial glucose level and significantly decreased BGR. NPS also induced a higher FOS-ir expression in STn neurons when compared to neurons in control rats that only received artificial cerebrospinal fluid (aCSF) before CChrc stimulation. In contrast, a selective NOS inhibitor such as Nomega-nitro-L-arginine methyl ester (L-NAME) in the STn before CChrc stimulation resulted in an increase of both, systemic glucose and BGR above control values. In this case, the number of FOS-ir positive neurons in the STn decreased when compared to control or to NPS experiments. FOS-ir expression in brainstem cells suggests that CChrc stimulation activates nitroxidergic pathways in the STn to regulate peripheral and central glucose homeostasis. The study of these functionally defined cells will be important to understand brain glucose homeostasis.
authors.affiliation: Centro Universitario de Investigaciones Biomédicas, Universidad de Colima, Colima, Col. 28045, Mexico.
authors.forename: M
authors.initials: M
authors.lastname: Lemus
authors.forename: S
authors.initials: S
authors.lastname: Montero
authors.forename: S
authors.initials: S
authors.lastname: Luquín
authors.forename: J
authors.initials: J
authors.lastname: García
authors.forename: E Roces
authors.initials: ER
authors.lastname: De Alvarez-Buylla
mesh_terms: D000818:Animals; D002344:Carotid Body / Q000187:drug effects / Q000378:metabolism*; D005786:Gene Expression Regulation / Q000187:drug effects; D005947:Glucose / Q000378:metabolism*; D006706:Homeostasis / Q000187:drug effects; D008297:Male; D009474:Neurons / Q000378:metabolism; D009569:Nitric Oxide / Q000378:metabolism*; D009599:Nitroprusside / Q000494:pharmacology; D010784:Photomicrography; D016760:Proto-Oncogene Proteins c-fos / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D012966:Sodium Cyanide / Q000494:pharmacology; D017552:Solitary Nucleus / Q000187:drug effects / Q000378:metabolism*; D013997:Time Factors
chemical_list: D016760:Proto-Oncogene Proteins c-fos; D009599:Nitroprusside; D009569:Nitric Oxide; D005947:Glucose; D012966:Sodium Cyanide