In situ primary metabolites localization on a rat brain section by chemical mass spectrometry imaging.
We describe here the detection and identification of 13 primary metabolites (AMP, ADP, ATP, UDP-GlcNAc, ...) directly from rat brain sections by chemical mass spectrometry imaging. Matrix-assisted laser desorption/ionization tandem mass spectrometry (MALDI-MS/MS) was combined with 9-aminoacridine as a powerful matrix. We also demonstrate that a new robotic sprayer allows us to homogeneously coat the surface with the matrix, enabling the acquisition of chemical images at a 50 microm resolution, leading us to precisely and simultaneously localize each metabolite over the tissue surface. These experiments open a new field of investigation for chemical mass spectrometry imaging and are of great interest for both chemists and biologists.
Benabdellah
Farida
F
Institut de Chimie des Substances Naturelles, CNRS UPR 2301, Avenue de la Terrasse 91198 Gif-sur-Yvette Cedex, France.
Touboul
David
D
Brunelle
Alain
A
Laprévote
Olivier
O
D000585:Aminacrine / Q000737:chemistry*; D000818:Animals; D001921:Brain / Q000378:metabolism*; D055442:Metabolome; D051381:Rats; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D053719:Tandem Mass Spectrometry / Q000379:methods*
D000585:Aminacrine