title: A novel method developed for acetylcholine detection in royal jelly by using capillary electrophoresis coupled with electrogenerated chemiluminescence based on a simple reaction.
abstract: A novel method for highly sensitive detection of acetylcholine in royal jelly was proposed by using CE coupled with electrogenerated chemiluminescence (ECL). Acetylcholine, which could not react with Tris(2,2'-bipyridine)ruthenium(II) to strengthen its ECL signals, decomposed into trimethylamine and strengthened the ECL signals sharply when it was heated to its melting point. This reaction needed no additional reagent and it was mild, simple, stable and rapid, without any side reaction. By combining the above process with CE separation technique, trimethylamine in royal jelly was completely separated from interfering substances and was successfully detected within 4 min. The limit of detection for acetylcholine was found to be 6.3 x 10(-8) g/mL with a signal-to-noise ratio of 3:1. Acetylcholine in the royal jelly was detected to be 912+/-58 microg/g. The recoveries of acetylcholine chloride in the sample were in the ranges of 92-106%. The coefficients of variation for intra-day and inter-day reproducibility were equal to or less than 4.9 and 6.8%, respectively.
authors.affiliation: School of Public Health, Southeast University, Dingjiaqiao, Nanjing, Jiangsu Province, P. R. China. wei_wei98@163.com
authors.forename: Wei
authors.initials: W
authors.lastname: Wei
authors.forename: Min
authors.initials: M
authors.lastname: Wei
authors.forename: Xuejun
authors.initials: X
authors.lastname: Kang
authors.forename: Huihua
authors.initials: H
authors.lastname: Deng
authors.forename: Zuhong
authors.initials: Z
authors.lastname: Lu
mesh_terms: D015082:2,2'-Dipyridyl / Q000031:analogs & derivatives / Q000737:chemistry; D000109:Acetylcholine / Q000032:analysis*; D019075:Electrophoresis, Capillary / Q000379:methods*; D005227:Fatty Acids / Q000737:chemistry*; D008163:Luminescent Measurements / Q000379:methods*; D008744:Methylamines / Q000032:analysis; D009942:Organometallic Compounds / Q000737:chemistry; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D000099219:Royal Jelly
chemical_list: D015082:2,2'-Dipyridyl; D000109:Acetylcholine; D005227:Fatty Acids; D008744:Methylamines; D009942:Organometallic Compounds; D000099219:Royal Jelly; C103599:bis(bipyridyl)ruthenium(II); C023336:trimethylamine