Increased potency of a novel D-beta-naphthylalanine-substituted antimicrobial peptide against fluconazole-resistant fungal pathogens.
The antifungal activities of the known antimicrobial peptide, P-113, as well as a new type of Trp-rich peptide, Ac-KWRRWVRWI-NH(2), Pac-525, and its modified peptide, D-Nal-Pac-525, were determined using the broth microdilution method in three different media. All peptides had similar activities against yeast pathogens in low-salt LYM media. However, only D-Nal-Pac-525 retained its antifungal activity in the media containing high concentrations of salt. Hence, D-Nal-Pac-525 has the potential of becoming a promising antifungal agent, especially for fungal pathogens with intrinsic resistance to fluconazole.
Wang
Chih-Wei
CW
Division of Infectious Diseases, National Health Research Institutes, Miaoli, Taiwan.
Yip
Bak-Sau
BS
Cheng
His-Tsung
HT
Wang
An-Huei
AH
Chen
Heng-Li
HL
Cheng
Jya-Wei
JW
Lo
Hsiu-Jung
HJ
D019943:Amino Acid Substitution / Q000235:genetics*; D000935:Antifungal Agents / Q000494:pharmacology*; D023181:Antimicrobial Cationic Peptides / Q000235:genetics / Q000494:pharmacology*; D025141:Drug Resistance, Fungal; D015725:Fluconazole / Q000494:pharmacology*; D005658:Fungi / Q000187:drug effects*; D008826:Microbial Sensitivity Tests / Q000379:methods; D015091:beta-Alanine / Q000031:analogs & derivatives* / Q000235:genetics
D000935:Antifungal Agents; D023181:Antimicrobial Cationic Peptides; D015091:beta-Alanine; C063914:beta-naphthylalanine; D015725:Fluconazole