Down-regulated miR-9 and miR-433 in human gastric carcinoma.
BACKGROUND
MircoRNAs(miRNAs) are short, endogenously non-coding RNAs. The abnormal expression of miRNAs may be valuable for the diagnosis and treatment of tumors.


METHODS
To screening the special miRNAs in gastric carcinoma, expression level of miRNAs in gastric carcinoma and normal gaster samples were detected by miRNA gene chip. Then, the expressions of miR-9 and miR-433 in gastric carcinoma tissue and SGC7901 cell line were validated by qRT-PCR. GRB2 and RAB34, targets of miR-433 and miR-9 respectively, were detected by Western blot.


RESULTS
We found 19 miRNAs and 7 miRNAs were down-regulated and up-regulated respectively. Compared with normal gaster samples, our data showed that miR-9 and miR-433 were down-regulated in gastric carcinoma. Meanwhile, we also found that miR-433 and miR-9 regulated the expression levels of GRB2 and RAB34 respectively.


CONCLUSIONS
Our data show miR-9 and miR-433 was down-regulated in gastric carcinoma. The targets of miR-433 and miR-9 were tumor-associated proteins GRB2 and RAB34 respectively. This result provided the related information of miRNAs in gastric carcinoma.
Luo
Hongchun
H
Department of Gastroenterology, The Second Affiliated Hospital, Chongqing Medical University, Chongqing 400010, PR China. wdlz129@sina.com.
Zhang
Hongbin
H
Zhang
Zhenzhen
Z
Zhang
Xia
X
Ning
Bo
B
Guo
Jinjun
J
Nie
Na
N
Liu
Bo
B
Wu
Xiaoling
X
D015153:Blotting, Western; D015536:Down-Regulation / Q000235:genetics*; D051380:GRB2 Adaptor Protein / Q000235:genetics / Q000378:metabolism; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic / Q000502:physiology*; D006801:Humans; D008156:Luciferases / Q000378:metabolism; D035683:MicroRNAs / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012334:RNA, Neoplasm / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013274:Stomach Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D014162:Transfection; D014407:Tumor Cells, Cultured; D020691:rab GTP-Binding Proteins / Q000235:genetics / Q000378:metabolism
D051380:GRB2 Adaptor Protein; C495948:GRB2 protein, human; D035683:MicroRNAs; D012333:RNA, Messenger; D012334:RNA, Neoplasm; D008156:Luciferases; C474116:RAB39A protein, human; D020691:rab GTP-Binding Proteins