Quantitative analysis of gene expression in a single cell by qPCR.
We developed a quantitative PCR method featuring a reusable single-cell cDNA library immobilized on beads for measuring the expression of multiple genes in a single cell. We used this method to analyze multiple cDNA targets (from several copies to several hundred thousand copies) with an experimental error of 15.9% or less. This method is sufficiently accurate to investigate the heterogeneity of single cells.
Taniguchi
Kiyomi
K
Hitachi Central Research Laboratory, Tokyo, Japan.
Kajiyama
Tomoharu
T
Kambara
Hideki
H
D018076:DNA, Complementary / Q000235:genetics; D015870:Gene Expression; D015723:Gene Library; D016133:Polymerase Chain Reaction / Q000379:methods* / Q000706:statistics & numerical data; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics
D018076:DNA, Complementary; D012333:RNA, Messenger