title: Role of Inn1 and its interactions with Hof1 and Cyk3 in promoting cleavage furrow and septum formation in S. cerevisiae.
abstract: Cytokinesis requires coordination of actomyosin ring (AMR) contraction with rearrangements of the plasma membrane and extracellular matrix. In Saccharomyces cerevisiae, new membrane, the chitin synthase Chs2 (which forms the primary septum [PS]), and the protein Inn1 are all delivered to the division site upon mitotic exit even when the AMR is absent. Inn1 is essential for PS formation but not for Chs2 localization. The Inn1 C-terminal region is necessary for localization, and distinct PXXP motifs in this region mediate functionally important interactions with SH3 domains in the cytokinesis proteins Hof1 (an F-BAR protein) and Cyk3 (whose overexpression can restore PS formation in inn1Delta cells). The Inn1 N terminus resembles C2 domains but does not appear to bind phospholipids; nonetheless, when overexpressed or fused to Hof1, it can provide Inn1 function even in the absence of the AMR. Thus, Inn1 and Cyk3 appear to cooperate in activating Chs2 for PS formation, which allows coordination of AMR contraction with ingression of the cleavage furrow.
authors.affiliation: Department of Genetics, Stanford University School of Medicine, Stanford, CA 94305, USA.
authors.forename: Ryuichi
authors.initials: R
authors.lastname: Nishihama
authors.forename: Jennifer H
authors.initials: JH
authors.lastname: Schreiter
authors.forename: Masayuki
authors.initials: M
authors.lastname: Onishi
authors.forename: Elizabeth A
authors.initials: EA
authors.lastname: Vallen
authors.forename: Julia
authors.initials: J
authors.lastname: Hanna
authors.forename: Katarina
authors.initials: K
authors.lastname: Moravcevic
authors.forename: Margaret F
authors.initials: MF
authors.lastname: Lippincott
authors.forename: Haesun
authors.initials: H
authors.lastname: Han
authors.forename: Mark A
authors.initials: MA
authors.lastname: Lemmon
authors.forename: John R
authors.initials: JR
authors.lastname: Pringle
authors.forename: Erfei
authors.initials: E
authors.lastname: Bi
mesh_terms: D000818:Animals; D018797:Cell Cycle Proteins / Q000235:genetics / Q000378:metabolism*; D002687:Chitin Synthase / Q000235:genetics / Q000378:metabolism; D048749:Cytokinesis / Q000502:physiology; D003599:Cytoskeleton / Q000378:metabolism / Q000648:ultrastructure; D004789:Enzyme Activation; D006801:Humans; D008869:Microtubule-Associated Proteins / Q000235:genetics / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000378:metabolism / Q000648:ultrastructure; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D018797:Cell Cycle Proteins; C418803:Cyk3 protein, S cerevisiae; C116847:HOF1 protein, S cerevisiae; C528682:Inn1 protein, S cerevisiae; D008869:Microtubule-Associated Proteins; D011993:Recombinant Fusion Proteins; D029701:Saccharomyces cerevisiae Proteins; D002687:Chitin Synthase; C400821:chitin synthase 2