Iron depletion decreases proliferation and induces apoptosis in a human colonic adenocarcinoma cell line, Caco2.
Iron is essential for maintaining cellular metabolism of most organisms. Iron chelators such as desferrioxamine have been used clinically in the treatment of iron overload diseases. In the present study, we used human colon adenocarcinoma cells as a proliferating cell model to validate that desferrioxamine inhibits cell proliferation and induces apoptosis. Proteomic analysis revealed that proteins involved in cell proliferation, signal transduction, metabolism and protein synthesis were significantly regulated by the availability of iron, rendering a close correlation between cell apoptosis and the disturbance of mitochondrial, signaling and metabolic pathways. These results provide new insights into the mechanisms of cell proliferation inhibition attributed to iron depletion.
Sun
Xuesong
X
Institute of Life and Health Engineering, Jinan University, Guangzhou, China.
Ge
Ruiguang
R
Cai
Zongwei
Z
Sun
Hongzhe
H
He
Qing-Yu
QY
D017209:Apoptosis; D018938:Caco-2 Cells; D049109:Cell Proliferation / Q000187:drug effects*; D003676:Deferoxamine / Q000494:pharmacology*; D015536:Down-Regulation; D006801:Humans; D007501:Iron / Q000378:metabolism; D007502:Iron Chelating Agents / Q000494:pharmacology*; D000090463:Iron Deficiencies; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D020543:Proteome / Q000187:drug effects*; D017262:Siderophores / Q000494:pharmacology; D015854:Up-Regulation
D007502:Iron Chelating Agents; D020543:Proteome; D017262:Siderophores; D007501:Iron; D003676:Deferoxamine