title: Relevance of testicular sperm DNA oxidation for the outcome of ovum donation cycles.
abstract: OBJECTIVE
  To determine the relevance of sperm DNA oxidation caused by free radicals in samples obtained via testicular biopsies by means of flow cytometry by correlating the measurements of 8-hydroxy-2'-deoxyguanosine (8-OHdG) with embryo features and pregnancy achievement.
  
  
  METHODS
  Prospective cross-sectional study.
  
  
  METHODS
  Private University-affiliated setting.
  
  
  METHODS
  Fifty-seven azoospermic patients undergoing testicular sperm extraction (TESE) were analyzed in their corresponding assisted reporductive technology cycles using ovum donation to standardize female's characteristics.
  
  
  METHODS
  None.
  
  
  METHODS
  Quantification of the adduct 8-OHdG in testicular tissue samples, and its effect on markers of embryo quality and reproductive success, and its relevance as marker of TESE sperm quality.
  
  
  RESULTS
  We found the status of sperm DNA oxidation to have very little clinical relevance for several parameters of embryo quality, fertilization rates, early (days 2-3) and late (days 5-6) development, and achievement of pregnancy.
  
  
  CONCLUSIONS
  The TESE obtained cells from azoospermic males do not possess a DNA oxidation status of significant importance in the success of assisted reproduction treatments, as determined by 8-OHdG measurement of each category of cell ploidy.
authors.affiliation: Instituto Universitario IVI and FIVI, (Fundación IVI), Valencia, Spain.
authors.forename: Cristóbal
authors.initials: C
authors.lastname: Aguilar
authors.forename: Marcos
authors.initials: M
authors.lastname: Meseguer
authors.forename: Sandra
authors.initials: S
authors.lastname: García-Herrero
authors.forename: Manuel
authors.initials: M
authors.lastname: Gil-Salom
authors.forename: José E
authors.initials: JE
authors.lastname: O'Connor
authors.forename: Nicolás
authors.initials: N
authors.lastname: Garrido
mesh_terms: D000328:Adult; D053713:Azoospermia / Q000628:therapy; D004247:DNA / Q000378:metabolism*; D004249:DNA Damage / Q000502:physiology; D005260:Female; D006801:Humans; D008297:Male; D018587:Oocyte Donation / Q000379:methods* / Q000592:standards; D010084:Oxidation-Reduction; D018384:Oxidative Stress / Q000502:physiology*; D011003:Ploidies; D011247:Pregnancy; D020554:Sperm Injections, Intracytoplasmic / Q000379:methods* / Q000592:standards; D053625:Sperm Retrieval; D013094:Spermatozoa / Q000378:metabolism*; D016896:Treatment Outcome
chemical_list: D004247:DNA