title: Surface-enhanced Raman scattering as a tool to probe cytochrome P450-catalysed substrate oxidation.
abstract: Surface-enhanced Raman scattering was used as a spectroscopic tool to investigate the changes brought upon cytochrome P450BSss after fatty acid binding. Differences in the spectra of substrate-free and substrate-bound enzyme were observed indicating the potential for this method to be used in the screening of P450 substrates. In particular, the binding characteristics of myristic acid, an inherent substrate, and hydroxylauric acid, a product of fatty acid oxidation, towards P450BSss in the presence of H(2)O(2) were investigated. Specific spectral changes could be assigned to changes in the heme environment only for myristic acid, indicating an occurrence of oxidation process characteristic for the enzymatic substrate.
authors.affiliation: Institute for Analytical Sciences (ISAS), Bunsen-Kirchhoff-Str. 11, 44139, Dortmund, Germany.
authors.forename: Elena
authors.initials: E
authors.lastname: Bailo
authors.forename: Ljiljana
authors.initials: L
authors.lastname: Fruk
authors.forename: Christof M
authors.initials: CM
authors.lastname: Niemeyer
authors.forename: Volker
authors.initials: V
authors.lastname: Deckert
mesh_terms: D001665:Binding Sites; D055162:Biocatalysis; D003577:Cytochrome P-450 Enzyme System / Q000737:chemistry / Q000378:metabolism*; D006861:Hydrogen Peroxide / Q000737:chemistry; D007850:Lauric Acids / Q000737:chemistry*; D008958:Models, Molecular; D019814:Myristic Acid / Q000737:chemistry*; D010084:Oxidation-Reduction; D012680:Sensitivity and Specificity; D013059:Spectrum Analysis, Raman / Q000295:instrumentation / Q000379:methods*; D013499:Surface Properties
chemical_list: D007850:Lauric Acids; D019814:Myristic Acid; C031263:12-hydroxydodecanoic acid; D003577:Cytochrome P-450 Enzyme System; D006861:Hydrogen Peroxide