title: Panton-Valentine leukocidin-positive Staphylococcus aureus skin and soft tissue infections among children in an emergency department in Madrid, Spain.
abstract: Fifty-three children who attended the emergency department with community-associated (CA) Staphylococcus aureus skin and soft tissue infections (SSTIs) were enrolled in the study. Seven cases of infection (13.2%) were due to methicillin-resistant S. aureus (MRSA). Twelve of 46 available isolates (26.1%) were Panton-Valentine leukocidin (PVL)-positive. PVL-positive S. aureus SSTIs were more frequently associated with abscesses and cellulitis (75% vs. 38%, p 0.028), and more commonly required incision and drainage (75% vs. 21%, p 0.001). Most PVL-positive CA-MRSA isolates belonged to a single multilocus sequence type (ST8). In contrast, PVL-positive methicillin-susceptible S. aureus isolates belonged to four different sequence types (ST8, ST30, ST80, ST120).
authors.affiliation: Servicio de Microbiología, Hospital Universitario 12 de Octubre, Avenida de Cordoba sn, Madrid, Spain.
authors.forename: M
authors.initials: M
authors.lastname: Daskalaki
authors.forename: P
authors.initials: P
authors.lastname: Rojo
authors.forename: M
authors.initials: M
authors.lastname: Marin-Ferrer
authors.forename: M
authors.initials: M
authors.lastname: Barrios
authors.forename: J R
authors.initials: JR
authors.lastname: Otero
authors.forename: F
authors.initials: F
authors.lastname: Chaves
mesh_terms: D000293:Adolescent; D001427:Bacterial Toxins / Q000235:genetics*; D016009:Chi-Square Distribution; D002648:Child; D002675:Child, Preschool; D016521:Electrophoresis, Gel, Pulsed-Field; D004636:Emergency Service, Hospital; D005098:Exotoxins / Q000235:genetics*; D005260:Female; D006801:Humans; D007223:Infant; D007956:Leukocidins / Q000235:genetics*; D008297:Male; D016106:Methicillin Resistance; D055624:Methicillin-Resistant Staphylococcus aureus / Q000235:genetics; D016133:Polymerase Chain Reaction; D018461:Soft Tissue Infections / Q000453:epidemiology / Q000382:microbiology*; D013030:Spain; D013207:Staphylococcal Skin Infections / Q000453:epidemiology / Q000382:microbiology*; D013211:Staphylococcus aureus / Q000235:genetics*
chemical_list: D001427:Bacterial Toxins; D005098:Exotoxins; D007956:Leukocidins; C078284:Panton-Valentine leukocidin