[Structural-functional study of glycine-and-proline-containing peptides (glyprolines) as potential neuroprotectors].
A synthetic scheme for preparation of (Gly-Pro)n, (Pro-Gly)n (n = 2, 3), and (Pro-Gly-Pro)n (n = 1, 2) peptides was elaborated. The effect of the synthesized peptides and the Gly-Pro and Pro-Gly dipeptides on survival of cultured cells of PC12 rat pheochromocytoma was studied under the conditions of oxidative stress induced by brief incubation of the cells with hydrogen peroxide. Peptides of the general formula (Gly-Pro)n and the Pro-Gly-Pro peptide at a concentration of 0.2-100 microM were shown to decrease the number of damaged cells. The Gly-Pro peptide was the most active and decreased the number of damaged cells by 49% on average at a concentration of 100 microM.
Martynova
K V
KV
Andreeva
L A
LA
Klimova
P A
PA
Kirillova
Iu G
IuG
Shevchenko
V P
VP
Nagaev
I Iu
IIu
Shram
I Iu
IIu
Shvets
V I
VI
Miasoedov
N F
NF
D000818:Animals; D004305:Dose-Response Relationship, Drug; D006861:Hydrogen Peroxide / Q000494:pharmacology; D018696:Neuroprotective Agents / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D016877:Oxidants / Q000494:pharmacology; D018384:Oxidative Stress / Q000187:drug effects*; D016716:PC12 Cells; D010455:Peptides / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D051381:Rats
D018696:Neuroprotective Agents; D016877:Oxidants; D010455:Peptides; D006861:Hydrogen Peroxide