Systematic analysis of biochemical performance and the microbial community of an activated sludge process using ozone-treated sludge for sludge reduction.
Two lab-scale bioreactors (reactors 1 and 2) were employed to examine the changes in biological performance and the microbial community of an activated sludge process fed with ozonated sludge for sludge reduction. During the 122 d operation, the microbial activities and community in the two reactors were evaluated. The results indicated that, when compared with the conventional reactor (reactor 1), the reactor that was fed with the ozonated sludge (reactor 2) showed good removal of COD, TN and cell debris, without formation of any excess sludge. In addition, the protease activity and intracellular ATP concentration of reactor 2 were increased when compared to reactor 1, indicating that reactor 2 had a better ability to digest proteins and cell debris. DGGE analysis revealed that the bacterial communities in the two reactors were different, and that the dissimilarity of the bacterial population was nearly 40%. Reactor 2 also contained more protozoa and metazoa, which could graze on the ozone-treated sludge debris directly.
Yan
Sang-Tian
ST
Department of Chemical Engineering, Tsinghua University, Beijing 100084, China.
Zheng
Hao
H
Li
An
A
Zhang
Xue
X
Xing
Xin-Hui
XH
Chu
Li-Bing
LB
Ding
Guoji
G
Sun
Xu-Lin
XL
Jurcik
Benjamin
B
D000251:Adenosine Triphosphatases / Q000378:metabolism; D000818:Animals; D001419:Bacteria / Q000235:genetics / Q000378:metabolism*; D020029:Base Pairing; D001673:Biodegradation, Environmental; D044822:Biodiversity; D019149:Bioreactors / Q000382:microbiology / Q000469:parasitology; D004586:Electrophoresis; D056890:Eukaryota; D042541:Intracellular Space / Q000378:metabolism; D010126:Ozone / Q000737:chemistry*; D010447:Peptide Hydrolases / Q000378:metabolism; D010802:Phylogeny; D012722:Sewage / Q000382:microbiology*; D014865:Waste Disposal, Fluid
D012722:Sewage; D010126:Ozone; D010447:Peptide Hydrolases; D000251:Adenosine Triphosphatases