Generation of mice with a conditional allele for Ift172.
Ift172 encodes a gene product that is part of a complex that mediates intraflagellar transport (IFT), a process necessary for the genesis and maintenance of cilia. Genetic studies in mice have offered evidence that Ift172 also plays a role in hedgehog signaling. Disruption of Ift172 in mice is associated with lethality at about embryonic day 11, limiting studies to understand the role for Ift172 in later development and the adult. To further our understanding of the later roles of Ift172, we have generated mice with a conditional allele for Ift172. We have confirmed the phenotype of the disrupted allele by using CRE expression directed by the prx1 enhancer to disrupt the conditional Ift172 allele in the developing limb.
Howard
Paul W
PW
Department of Cell and Developmental Biology, L215, Oregon Health & Science University, Portland, OR 97239, USA.
Howard
Tiffani L
TL
Maurer
Richard A
RA
D048868:Adaptor Proteins, Signal Transducing; D000483:Alleles; D000818:Animals; D003598:Cytoskeletal Proteins; D004622:Embryo, Mammalian; D005121:Extremities / Q000196:embryology / Q000473:pathology; D018507:Gene Expression Regulation, Developmental; D018014:Gene Transfer Techniques; D005822:Genetic Vectors / Q000502:physiology; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics*; D017880:Limb Deformities, Congenital / Q000196:embryology / Q000235:genetics; D051379:Mice; D008822:Mice, Transgenic / Q000196:embryology / Q000235:genetics*; D016297:Mutagenesis, Site-Directed / Q000379:methods; D010641:Phenotype
D048868:Adaptor Proteins, Signal Transducing; D003598:Cytoskeletal Proteins; C479520:Ift172 protein, mouse; D047908:Intracellular Signaling Peptides and Proteins