Transcriptional regulation of wheat VER2 promoter in rice in response to abscisic acid, jasmonate, and light.
VER2 is a key gene associated with vernalization process in winter wheat. The expression of VER2 can be induced by low temperature treatment. To further understand how the expression of this gene is mediated by various external and internal factors, different lengths of the VER2 promoter region have been transcriptionally fused with a reporter gene, green fluorescence protein (GFP), and transformed into the model plant, rice (Oryza sativa L.). Using confocal and Western blot analyses, we determined several possible response elements in the promoter region, which could sense ABA, JA, and other environmental cues.
Feng
Hong
H
College of Life Science, Sichuan University, Chendu, China.
Xu
Wen-Zhong
WZ
Lin
Hong-Hui
HH
Chong
Kang
K
D000040:Abscisic Acid / Q000378:metabolism*; D003517:Cyclopentanes / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant / Q000528:radiation effects; D017930:Genes, Reporter; D008027:Light; D012275:Oryza / Q000235:genetics / Q000378:metabolism / Q000528:radiation effects*; D054883:Oxylipins / Q000378:metabolism*; D010940:Plant Proteins / Q000235:genetics*; D011401:Promoter Regions, Genetic; D014158:Transcription, Genetic / Q000528:radiation effects; D014908:Triticum / Q000235:genetics*
D003517:Cyclopentanes; D054883:Oxylipins; D010940:Plant Proteins; C011006:jasmonic acid; D000040:Abscisic Acid