Isolation of the new minor constituents dihydropyranochromone and furanocoumarin from fruits of Peucedanum alsaticum L. by high-speed counter-current chromatography.
A preparative high-speed counter-current chromatography (HSCCC) method was successfully used for isolation of two new minor compounds--alsaticol and alsaticocoumarin A. A two-phase solvent system composed of n-hexane-ethyl acetate-methanol-water (1:1:1:1) was developed. Compounds were obtained from the dichloromethane extract of Peucedanum alsaticum fruits and their identification was performed with NMR and MS methods. Optimized HSCCC offers a rapid method of obtaining new natural compounds.
Skalicka-Woźniak
Krystyna
K
Department of Pharmacognosy with Medicinal Plant Laboratory, Medical University of Lublin, 1 Chodzki Street, 20-093 Lublin, Poland. kskalicka@pharmacognosy.org
Mroczek
Tomasz
T
Garrard
Ian
I
Głowniak
Kazimierz
K
D019661:Apiaceae / Q000737:chemistry*; D002867:Chromones / Q000302:isolation & purification*; D003377:Countercurrent Distribution / Q000379:methods*; D005638:Fruit / Q000737:chemistry*; D011564:Furocoumarins / Q000302:isolation & purification*; D010936:Plant Extracts / Q000302:isolation & purification
D002867:Chromones; D011564:Furocoumarins; D010936:Plant Extracts; C543205:dihydropyranochromone