[Polymorphism of D10S1248, D2S441, D1S1677 in Hunan Han population].
OBJECTIVE
To establish a multiplex set including D10S1248, D2S441, D1S1677 and to investigate the genetic polymorphism of the three miniSTR in Hunan Han population.


METHODS
Three miniSTR loci of 186 unrelated individuals were amplified with different multiplex fluorescence-labeled primers. The amplified products were analyzed by ABI 310 Genetic Analyzer to identify genotype.


RESULTS
Each locus was successfully genotyped. Among the 186 individuals investigated, 9, 7 and 7 alleles, as well as 21, 19 and 15 genotypes were detected at D10S1248, D2S441 and D1S1677, respectively. No significant deviation from Hardy-Weinberg equilibrium was observed. The excluding probability of paternity and the discrimination power were 0,465, 0.491 and 0.361, as well as 0.886, 0.899 and 0.818 for D10S1248, D2S441 and D1S1677, respectively.


CONCLUSIONS
The miniSTR multiplex set can benefit forensic analysis of degraded samples. It has shown good polymorphism in Hunan Han population and can be used in personal identification and paternity test.
Li
Xia
X
Hebei Key Laboratory of Forensic Medicine, Department of Forensic Medicine, Hebei Medical University, Shijiazhuang 050017, China. yutongli116@163.com
Cong
Bin
B
Li
Shu-Jin
SJ
Bai
Xue
X
Xu
Li
L
Hou
Zhi-Ping
ZP
Guo
Xia
X
D000483:Alleles; D044466:Asian People / Q000235:genetics; D001483:Base Sequence; D002681:China / Q000208:ethnology; D053803:Forensic Genetics; D005828:Genetics, Population; D005838:Genotype; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic / Q000235:genetics*; D017422:Sequence Analysis, DNA; D020080:Tandem Repeat Sequences / Q000235:genetics*