title: Detection and quantification of hepatitis C virus (HCV) by MultiCode-RTx real-time PCR targeting the HCV 3' untranslated region.
abstract: A prototype, real-time reverse-transcription PCR assay, based on MultiCode-RTx technology, quantifying hepatitis C virus (HCV) RNA by targeting the HCV 3' untranslated region demonstrated linearity over 7 logs, with a good correlation between the quantitative results of this assay and the results of two commercially available comparator assays for 466 clinical specimens comprising all six HCV genotypes.
authors.affiliation: EraGen Biosciences, Inc., Madison, WI 53717, USA.
authors.forename: Elizabeth K
authors.initials: EK
authors.lastname: Mulligan
authors.forename: J J
authors.initials: JJ
authors.lastname: Germer
authors.forename: Max Q
authors.initials: MQ
authors.lastname: Arens
authors.forename: Krista L
authors.initials: KL
authors.lastname: D'Amore
authors.forename: Adrian
authors.initials: A
authors.lastname: Di Bisceglie
authors.forename: Nathan A
authors.initials: NA
authors.lastname: Ledeboer
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Moser
authors.forename: Andrew C
authors.initials: AC
authors.lastname: Newman
authors.forename: Andy K
authors.initials: AK
authors.lastname: O'Guin
authors.forename: Paul D
authors.initials: PD
authors.lastname: Olivo
authors.forename: Diane S
authors.initials: DS
authors.lastname: Podzorski
authors.forename: Kimberly A
authors.initials: KA
authors.lastname: Vaughan
authors.forename: Joseph D
authors.initials: JD
authors.lastname: Yao
authors.forename: Slava A
authors.initials: SA
authors.lastname: Elagin
authors.forename: Scott C
authors.initials: SC
authors.lastname: Johnson
mesh_terms: D020413:3' Untranslated Regions; D016174:Hepacivirus / Q000235:genetics / Q000302:isolation & purification*; D006526:Hepatitis C / Q000175:diagnosis* / Q000821:virology; D006801:Humans; D010949:Plasma / Q000821:virology; D012367:RNA, Viral / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D044967:Serum / Q000821:virology; D019562:Viral Load / Q000379:methods*
chemical_list: D020413:3' Untranslated Regions; D012367:RNA, Viral