Time-resolved tracking of a minimum gene expression system reconstituted in giant liposomes.
Individual expression: We describe a method that allows the observation of real-time gene expression in a large number of individual giant liposomes encapsulating identical genetic material. We followed the gene expression profiles from DNA and mRNA templates coding for different proteins. Although the average profiles of individual liposomes were similar to those measured in bulk solution, strong variability between individual liposomes was observed at both transcription and translation.
Saito
Hirohide
H
Department of Gene Mechanisms, Graduate School of Biostudies, Kyoto University, Kyoto, Japan. h-saito@kuchem.kyoto-u.ac.jp
Kato
Yusho
Y
Le Berre
Maël
M
Yamada
Ayako
A
Inoue
Tan
T
Yosikawa
Kenichi
K
Baigl
Damien
D
D004247:DNA / Q000378:metabolism; D015870:Gene Expression; D020869:Gene Expression Profiling; D049452:Green Fluorescent Proteins / Q000235:genetics; D008081:Liposomes / Q000737:chemistry* / Q000494:pharmacology; D012333:RNA, Messenger / Q000378:metabolism; D013997:Time Factors
D008081:Liposomes; D012333:RNA, Messenger; D049452:Green Fluorescent Proteins; D004247:DNA