Additive effects of insulin and muscle contraction on fatty acid transport and fatty acid transporters, FAT/CD36, FABPpm, FATP1, 4 and 6.
Insulin and muscle contraction increase fatty acid transport into muscle by inducing the translocation of FAT/CD36. We examined (a) whether these effects are additive, and (b) whether other fatty acid transporters (FABPpm, FATP1, FATP4, and FATP6) are also induced to translocate. Insulin and muscle contraction increased glucose transport and plasmalemmal GLUT4 independently and additively (positive control). Palmitate transport was also stimulated independently and additively by insulin and by muscle contraction. Insulin and muscle contraction increased plasmalemmal FAT/CD36, FABPpm, FATP1, and FATP4, but not FATP6. Only FAT/CD36 and FATP1 were stimulated in an additive manner by insulin and by muscle contraction.
Jain
Swati S
SS
Department of Human Health and Nutritional Sciences, University of Guelph, Guelph, Ontario, Canada.
Chabowski
A
A
Snook
Laelie A
LA
Schwenk
Robert W
RW
Glatz
Jan F C
JF
Luiken
Joost J F P
JJ
Bonen
Arend
A
D000818:Animals; D024442:Aspartate Aminotransferase, Mitochondrial / Q000378:metabolism; D001692:Biological Transport; D018955:CD36 Antigens / Q000378:metabolism*; D050612:Fatty Acid Transport Proteins / Q000378:metabolism*; D050556:Fatty Acid-Binding Proteins / Q000378:metabolism*; D051275:Glucose Transporter Type 4 / Q000378:metabolism; D007004:Hypoglycemic Agents / Q000494:pharmacology*; D007328:Insulin / Q000494:pharmacology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009119:Muscle Contraction / Q000187:drug effects / Q000502:physiology*
D018955:CD36 Antigens; C543322:FATP6 protein, mouse; D050612:Fatty Acid Transport Proteins; D050556:Fatty Acid-Binding Proteins; D051275:Glucose Transporter Type 4; D007004:Hypoglycemic Agents; D007328:Insulin; C493569:Slc27a1 protein, mouse; C493572:Slc27a4 protein, mouse; D024442:Aspartate Aminotransferase, Mitochondrial