Lung cancer xenografting alters microRNA profile but not immunophenotype.
Lung tumor xenografts grown in immunocompromised mice provide a renewable source of tumor tissue for research and a means to study individualized response to chemotherapy. Critical to this utility is verification that the xenograft cells retain core phenotypic characteristics of the original tumor. We compared eight non-small cell lung carcinomas with their corresponding xenografts grown in mice with severe combined immunodeficiency by way of histology, immunohistochemistry, and microRNA expression profiling. Six of the eight xenografts closely resembled their original tumor by light microscopy. The xenografts also largely retained key immunophenotypic features. With expression profiling of human microRNAs, however, xenografts clustered separately from the original tumors. While this may be partly due to contamination by non-neoplastic human and mouse stroma, the results suggest that miRNA expression may be altered in xenografts and that this possibility should be further evaluated.
Bogner
Paul N
PN
Department of Pathology, Roswell Park Cancer Institute, Elm and Carlton Streets, Buffalo, NY 14263, USA. paul.bogner@roswellpark.org
Patnaik
Santosh K
SK
Pitoniak
Rose
R
Kannisto
Eric
E
Repasky
Elizabeth
E
Hylander
Bonnie
B
Yendamuri
Sai
S
Ramnath
Nithya
N
D000818:Animals; D020869:Gene Expression Profiling; D008175:Lung Neoplasms / Q000235:genetics*; D051379:Mice; D016513:Mice, SCID; D035683:MicroRNAs / Q000235:genetics*; D023041:Xenograft Model Antitumor Assays
D035683:MicroRNAs