Plasmonic nanoprobes for SERS biosensing and bioimaging.
This article provides an overview of the development and application of plasmonic nanoprobes developed in our laboratory for biosensing and bioimaging. We describe the use of plasmonics surface-enhanced Raman scattering (SERS) gene probes for the detection of diseases using DNA hybridization to target biospecies (HIV gene, breast cancer genes etc.). For molecular imaging, we describe a hyperspectral surface-enhanced Raman imaging (HSERI) system that combines imaging capabilities with SERS detection to identify cellular components using Raman dye-labeled silver nanoparticles in cellular systems The detection of specific target DNA sequences associated with breast cancer using "molecular sentinel" nanoprobes and the use of a plasmonic nanosensor to monitor pH in single cells are presented and discussed.
Vo-Dinh
Tuan
T
Fitzpatrick Institute for Photonics, Departments of Biomedical Engineering and Chemistry, Duke University, Durham, NC 27708, USA. tuan.vodinh@duke.edu
Wang
Hsin-Neng
HN
Scaffidi
Jonathan
J
D004247:DNA / Q000737:chemistry; D006801:Humans; D053768:Metal Nanoparticles / Q000737:chemistry; D055095:Optics and Photonics / Q000295:instrumentation* / Q000379:methods*; D012834:Silver / Q000737:chemistry
D012834:Silver; D004247:DNA