Dynamics in the tomato root transcriptome on infection with the potato cyst nematode Globodera rostochiensis.
Plant parasitic nematodes infect roots and trigger the formation of specialized feeding sites by substantial reprogramming of the developmental process of root cells. In this article, we describe the dynamic changes in the tomato root transcriptome during early interactions with the potato cyst nematode Globodera rostochiensis. Using amplified fragment length polymorphism-based mRNA fingerprinting (cDNA-AFLP), we monitored 17 600 transcript-derived fragments (TDFs) in infected and uninfected tomato roots, 1-14 days after inoculation with nematode larvae. Six hundred and twenty-four TDFs (3.5%) showed significant differential expression on nematode infection. We employed GenEST, a computer program which links gene expression profiles generated by cDNA-AFLP and databases of cDNA sequences, to identify 135 tomato sequences. These sequences were grouped into eight functional categories based on the presence of genes involved in hormone regulation, plant pathogen defence response, cell cycle and cytoskeleton regulation, cell wall modification, cellular signalling, transcriptional regulation, primary metabolism and allocation. The presence of unclassified genes was also taken into consideration. This article describes the responsiveness of numerous tomato genes hitherto uncharacterized during infection with endoparasitic cyst nematodes. The analysis of transcriptome profiles allowed the sequential order of expression to be dissected for many groups of genes and the genes to be connected with the biological processes involved in compatible interactions between the plant and nematode.
Swiecicka
Magdalena
M
Department of Plant Genetics Breeding and Biotechnology, Warsaw University of Life Sciences, Poland.
Filipecki
Marcin
M
Lont
Dieuwertje
D
Van Vliet
Joke
J
Qin
Ling
L
Goverse
Aska
A
Bakker
Jaap
J
Helder
Johannes
J
D054458:Amplified Fragment Length Polymorphism Analysis; D000818:Animals; D016000:Cluster Analysis; D020224:Expressed Sequence Tags; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D018551:Solanum lycopersicum / Q000235:genetics* / Q000469:parasitology*; D018517:Plant Roots / Q000235:genetics* / Q000469:parasitology*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D015203:Reproducibility of Results; D020133:Reverse Transcriptase Polymerase Chain Reaction; D011198:Solanum tuberosum / Q000469:parasitology*; D014431:Tylenchoidea / Q000235:genetics / Q000502:physiology*; D015854:Up-Regulation / Q000235:genetics
D012333:RNA, Messenger