title: BacMam: versatile gene delivery technology for GPCR assays.
abstract: BacMam viruses are modified baculoviruses that contain mammalian expression cassettes for viral gene delivery and transient expression in mammalian cells. They are easily, inexpensively, and rapidly generated and provide a versatile solution for G protein-coupled receptor (GPCR) cell-based assay development. Using BacMam technology, target gene expression levels are easily controlled and simultaneous delivery of multiple genes is possible, for example, coexpression of a receptor and a G protein or a reporter gene. BacMam viruses are compatible with the GPCR cell-based assay formats typically used in high-throughput screening and provide an unparalleled level of experimental flexibility that is simply not possible when using stable recombinant cell lines.
authors.affiliation: Molecular Discovery Research, Biological Reagents and Assay Development, GlaxoSmithKline R&D, Collegeville, PA, USA.
authors.forename: Elizabeth A
authors.initials: EA
authors.lastname: Davenport
authors.forename: Parvathi
authors.initials: P
authors.lastname: Nuthulaganti
authors.forename: Robert S
authors.initials: RS
authors.lastname: Ames
mesh_terms: D016367:Baculoviridae / Q000235:genetics*; D001859:Bone Neoplasms / Q000235:genetics / Q000378:metabolism*; D018014:Gene Transfer Techniques; D005822:Genetic Vectors; D006801:Humans; D012516:Osteosarcoma / Q000235:genetics / Q000378:metabolism*; D043562:Receptors, G-Protein-Coupled / Q000235:genetics* / Q000378:metabolism; D014161:Transduction, Genetic; D014407:Tumor Cells, Cultured
chemical_list: D043562:Receptors, G-Protein-Coupled