title: miR-210 promotes osteoblastic differentiation through inhibition of AcvR1b.
abstract: Although microRNAs (miRNAs) are involved in many biological processes, the mechanisms whereby miRNAs regulate osteoblastic differentiation are poorly understood. Here, we found that BMP-4-induced osteoblastic differentiation of bone marrow-derived ST2 stromal cells was promoted and repressed after transfection of sense and antisense miR-210, respectively. A reporter assay demonstrated that the activin A receptor type 1B (AcvR1b) gene was a target for miR-210. Furthermore, inhibition of transforming growth factor-beta (TGF-beta)/activin signaling in ST2 cells with SB431542 promoted osteoblastic differentiation. We conclude that miR-210 acts as a positive regulator of osteoblastic differentiation by inhibiting the TGF-beta/activin signaling pathway through inhibition of AcvR1b.
authors.affiliation: Division of Functional Genomics and Systems Medicine, Research Center for Genomic Medicine, Saitama Medical University, Hidaka, Japan.
authors.forename: Yosuke
authors.initials: Y
authors.lastname: Mizuno
authors.forename: Yoshimi
authors.initials: Y
authors.lastname: Tokuzawa
authors.forename: Yuichi
authors.initials: Y
authors.lastname: Ninomiya
authors.forename: Ken
authors.initials: K
authors.lastname: Yagi
authors.forename: Yukiko
authors.initials: Y
authors.lastname: Yatsuka-Kanesaki
authors.forename: Tatsuo
authors.initials: T
authors.lastname: Suda
authors.forename: Toru
authors.initials: T
authors.lastname: Fukuda
authors.forename: Takenobu
authors.initials: T
authors.lastname: Katagiri
authors.forename: Yasumitsu
authors.initials: Y
authors.lastname: Kondoh
authors.forename: Tomoyuki
authors.initials: T
authors.lastname: Amemiya
authors.forename: Hideo
authors.initials: H
authors.lastname: Tashiro
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Okazaki
mesh_terms: D030201:Activin Receptors, Type I / Q000037:antagonists & inhibitors* / Q000378:metabolism; D000818:Animals; D055415:Bone Morphogenetic Protein 4 / Q000378:metabolism; D002454:Cell Differentiation; D002478:Cells, Cultured; D051379:Mice; D035683:MicroRNAs / Q000378:metabolism*; D010006:Osteoblasts / Q000166:cytology* / Q000378:metabolism; D015398:Signal Transduction; D014162:Transfection; D016212:Transforming Growth Factor beta / Q000378:metabolism
chemical_list: D055415:Bone Morphogenetic Protein 4; D035683:MicroRNAs; D016212:Transforming Growth Factor beta; D030201:Activin Receptors, Type I