The influence of different glycosylation patterns on factor VII biological activity.
In this study the bioactivity of three differently glycosylated blood coagulation factor VII (FVII) variants (human plasma FVII, recombinant human FVII produced in CHO and BHK cell cultures) were analyzed and compared. Surface plasmon resonance studies of FVII interaction with soluble and full length TF together with FVII autoactivation assays revealed that BHK-derived FVII has the highest bioactivity, while human plasma and CHO-derived FVII showed very similar bioactivity. The affinity of FVII variants to TF correlates with FVII autoactivation rates--the higher the affinity, the faster the autoactivation rate.
Sutkeviciute
Ieva
I
Department of Chemistry and Bioengineering, Vilnius Gediminas Technical University, Vilnius, Lithuania. ieva.sutkeviciute@gmail.com
Mistiniene
Edita
E
Sereikaite
Jolanta
J
Bumelis
Vladas A
VA
D000818:Animals; D016466:CHO Cells; D002460:Cell Line; D002851:Chromatography, High Pressure Liquid; D006224:Cricetinae; D003412:Cricetulus; D005167:Factor VII / Q000235:genetics / Q000378:metabolism*; D006031:Glycosylation; D006801:Humans; D007700:Kinetics; D011485:Protein Binding; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism*; D020349:Surface Plasmon Resonance; D013925:Thromboplastin / Q000378:metabolism
D011994:Recombinant Proteins; D005167:Factor VII; D013925:Thromboplastin