Distribution of elongation factor-1alpha in larval tissues of the fall armyworm, Spodoptera frugiperda.
Elongation factor-1alpha (EF-1alpha) promotes the delivery of aminoacyl-tRNA to the acceptor site of the ribosome during protein synthesis. The enzyme has a number of additional functions, including regulation of apoptosis and interaction with the cytoskeleton. We determined the distribution of EF-1alpha in larval tissues of the fall armyworm, Spodoptera frugiperda , with a monoclonal antibody generated to EF-1alpha from Sf21 cells, a cell line developed from ovarian tissue of S. frugiperda. Enzyme-linked immunosorbent assay showed that EF-1alpha comprised 1.9-9.9% of the total protein within the tissues that were examined, which included fat body, Malpighian tubules, midgut, muscle, salivary glands, trachea, and ventral nerve cord. To a certain extent, EF-1alpha concentrations reflected the expected metabolic activity level of each of the represented tissues. Closer examination by immunofluorescence microscopy revealed that EF-1alpha concentrations varied among different cell types within a given tissue, i.e. midgut columnar epithelial cells yielded strong signals, while goblet cells failed to react with the EF-1alpha-specific antibody.
Habibi
Javad
J
Department of Internal Medicine, University of Missouri, Columbia, MO, USA. habibij@health.missouri.edu
Goodman
Cynthia L
CL
Stuart
Melissa K
MK
D000818:Animals; D015153:Blotting, Western; D004797:Enzyme-Linked Immunosorbent Assay; D005786:Gene Expression Regulation; D007814:Larva / Q000378:metabolism; D008856:Microscopy, Fluorescence; D020648:Peptide Elongation Factor 1 / Q000378:metabolism*; D018411:Spodoptera / Q000378:metabolism*
D020648:Peptide Elongation Factor 1