Gene expression profiling of mice liver tissue after intragastric administration of Chinese nutgall extract.
Kunming mice (male, weight 20 +/- 2g) were daily intragastric administration of Chinese nutgall extract (0.2 mL/10 g body weight, equal to 8 g Chinese nutgall material/1 kg body weight) for 30 days. Liver tissue mRNA were extracted from normal control group and Chinese nutgall treated group respectively, then were reversely transcribed to cDNA with dUTP labeled by different fluorescence (Cy3, Cy5) as hybridization probes. Both of the cDNA probes were mixed equally in 20 microL of hybridization solution and hybridized with mice complete genome oligonucleotide microarray. The fluorescent signals were acquired by laser scanner and analyzed by GenePix Pro 4.0 software. The biological function analysis and the pathway analysis of differentially expressed genes were performed according to Gene Ontology database. As a result, there were 461 genes differentially expressed in Chinese nutgall treated group, in which 373 genes were function-known and the others were function-unknown. Among the 461 genes, 267 genes were up-regulated and the others were down-regulated. The differentially expressed genes were involved in metabolism, DNA binding and transcription, protein synthesis and modification, cell cytoskeleton and cell adhesion, cell cycle and differentiation, ion channels and transporters, signal transduction, immune response and apoptosis of liver cell. The presented work might be quite important for understanding the pathogenic mechanisms of liver injury induced by Chinese nutgall.
Han
Feng Mei
FM
Hubei Province Key Laboratory of Traditional Chinese Medicine & Biotechnology, Hubei University, Wuhan 430062, China.
Zhang
Xiao Ming
XM
Xia
Qi Song
QS
Wang
Jun Jun
JJ
Chen
Yong
Y
D000818:Animals; D002232:Carbocyanines / Q000494:pharmacology; D056486:Chemical and Drug Induced Liver Injury / Q000209:etiology; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D020869:Gene Expression Profiling; D017403:In Situ Hybridization; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D051379:Mice; D046228:Microarray Analysis; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D012333:RNA, Messenger / Q000187:drug effects / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction / Q000187:drug effects
D002232:Carbocyanines; D004365:Drugs, Chinese Herbal; D012333:RNA, Messenger; C085321:cyanine dye 5