Investigation of biological activity of polar extracts isolated from Phlomis crinita Cav ssp. mauritanica Munby.
The lyophilized infusion, the methanol, the ethyl acetate, and the total oligomer flavonoid (TOF)-enriched extracts prepared from the dried leaves of Phlomis crinita Cav. ssp. mauritanica Munby were investigated for the contents of flavonoids, tannins, coumarines and steroids. Antibacterial activity was investigated toward five bacterial strains. An inhibitory effect was observed against Staphyllococcus aureus and Enterococcus feacalis, and the minimal inhibitory concentrations ranged from 2.5 to 5 mg/mL of extract. The tested extracts exhibit an important free radical scavenging activity toward the 1,1-diphenyl 2-picrylhydrazyl (DPPH) free radical; with IC(50) values of 30.5, 6, 32, and 31.5 microg/mL, respectively, in the presence of lyophilized infusion, the TOF, the methanol, and the ethyl acetate extracts. Genotoxic and antigenotoxic properties of the different extracts were studied by using the SOS chromotest with Escherichia coli PQ37. The lyophilized infusion and TOF extracts obtained from P. crinita ssp. mauritanica showed no genotoxicity, whereas methanol and ethyl acetate extracts are considered as marginally genotoxic. On the other hand, we showed that each extract inhibited the mutagenicity induced by aflatoxin B1 (AFB1) (10 microg/assay) and nifuroxazide (NF) (10 microg/assay). The ethyl acetate extract showed the strongest level of protection toward the genotoxicity induced by both directly and indirectly genotoxic NF and AFB1. These tests proved that the lyophilized infusion possesses an antiradical activity likewise, it showed no genotoxic effect; that is why we choose this extract to assess its antiulcerogenic activity by using an ethanol-induced ulcerogenesis model in the rat. This test demonstrates that 300 mg/kg of a P. crinita ssp. mauritanica lyophilized infusion was more effective than the reference compound, cimetidine.
Limem-Ben Amor
Ilef
I
Laboratory of Cellular and Molecular Biology, Faculty of Dental Medicine, Monastir, Tunisia.
Skandrani
Ines
I
Boubaker
Jihed
J
Ben Sghaïer
Mohamed
M
Neffati
Aicha
A
Bhouri
Wissem
W
Bouhlel
Ines
I
Chouchane
Nabil
N
Kilani
Soumaya
S
Guedon
Emmanuel
E
Ghoul
Mohamed
M
Ghedira
Kamel
K
Chekir-Ghedira
Leila
L
D000818:Animals; D000900:Anti-Bacterial Agents / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology*; D000897:Anti-Ulcer Agents / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology*; D016587:Antimutagenic Agents / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology; D002927:Cimetidine / Q000494:pharmacology; D005260:Female; D016166:Free Radical Scavengers / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology; D005612:Freeze Drying; D020128:Inhibitory Concentration 50; D008297:Male; D008826:Microbial Sensitivity Tests; D009152:Mutagenicity Tests; D031345:Phlomis / Q000737:chemistry*; D010936:Plant Extracts / Q000008:administration & dosage / Q000494:pharmacology*; D018515:Plant Leaves; D051381:Rats
D000900:Anti-Bacterial Agents; D000897:Anti-Ulcer Agents; D016587:Antimutagenic Agents; D016166:Free Radical Scavengers; D010936:Plant Extracts; D002927:Cimetidine