Synthesis of 9-beta-d-arabinofuranosylguanine by combined use of two whole cell biocatalysts.
Unlike the preparation of other purine nucleosides, transglycosylation from a pyrimidine nucleoside and guanine is difficult because of the low solubility of this base. Thus, another strategy, based on the coupled action of two whole cell biocatalyzed reactions, transglycosylation and deamination, was used. Enterobacter gergoviae and Arthrobacter oxydans were employed to synthesize 9-beta-d-arabinofuranosylguanine (AraG), an efficient anti leukemic drug.
Médici
Rosario
R
Biotransformations Laboratory, Universidad Nacional de Quilmes. R.S. Peña 352, 1876 Bernal, Buenos Aires, Argentina.
Iribarren
Adolfo M
AM
Lewkowicz
Elizabeth S
ES
D000970:Antineoplastic Agents / Q000138:chemical synthesis / Q000494:pharmacology*; D001087:Arabinonucleosides / Q000138:chemical synthesis* / Q000494:pharmacology; D001173:Arthrobacter / Q000378:metabolism; D002384:Catalysis; D002460:Cell Line; D045744:Cell Line, Tumor; D002626:Chemistry, Pharmaceutical / Q000379:methods*; D015195:Drug Design; D004354:Drug Screening Assays, Antitumor; D004754:Enterobacter / Q000378:metabolism; D006031:Glycosylation; D006801:Humans; D007938:Leukemia / Q000188:drug therapy*; D008956:Models, Chemical
D000970:Antineoplastic Agents; D001087:Arabinonucleosides; C028771:9-arabinofuranosylguanine