Effect of titanium surface modified by plasma energy source on genotoxic response in vitro.
Titanium (Ti) is currently the most widely used material for the manufacture of orthopedic and dental implants. Changes in the surface of commercial pure Ti (cp Ti) can determine the functional response of cells, and is therefore a critical factor for the success of the implant. However, the genotoxicity of titanium surfaces has been poorly studied. Thus, the purpose of this study was to evaluate the genotoxic potential of a new titanium surface developed by plasma treatment using argon-ion bombardment and compare it with an untreated titanium surface. Accordingly, comet assay, analysis of chromosomal aberrations (CAs), and Cytokinesis Block Micronucleus (CBMN) assay were carried out, using CHO-K1 (Chinese hamster ovary) cells grown on both titanium surfaces. Our results show that the untreated titanium surface caused a significant increase in % tail moment, in the number of cells with CAs, tetraploidy, micronucleus frequency, and other nuclear alterations when compared with the negative control and with the plasma-treated titanium surface. This difference may be attributed to increased surface roughness and changes in titanium oxide layer thickness.
Tavares
Joana Cristina Medeiros
JC
Department of Cell Biology and Genetics, Bioscience Center (BC), Universidade Federal do Rio Grande do Norte (UFRN), Natal-RN, ZC 59072-970, Brazil.
Cornélio
Deborah Afonso
DA
da Silva
Naisandra Bezerra
NB
de Moura
Carlos Eduardo Bezerra
CE
de Queiroz
Jana Dara Freires
JD
Sá
Juliana Carvalho
JC
Alves
Clodomiro
C
de Medeiros
Silvia Regina Batistuzzo
SR
D000818:Animals; D001128:Argon; D016466:CHO Cells; D002467:Cell Nucleus / Q000187:drug effects / Q000473:pathology; D002470:Cell Survival / Q000187:drug effects; D002869:Chromosome Aberrations / Q000139:chemically induced*; D020099:Coated Materials, Biocompatible / Q000528:radiation effects; D020552:Comet Assay; D006224:Cricetinae; D003412:Cricetulus; D007477:Ions; D048629:Micronuclei, Chromosome-Defective / Q000139:chemically induced*; D015162:Micronucleus Tests; D009153:Mutagens / Q000528:radiation effects / Q000633:toxicity*; D013499:Surface Properties / Q000528:radiation effects; D014025:Titanium / Q000528:radiation effects / Q000633:toxicity*
D020099:Coated Materials, Biocompatible; D007477:Ions; D009153:Mutagens; D001128:Argon; D014025:Titanium