Methods for detection and characterization of lipases: A comprehensive review.
Microbial lipases are very prominent biocatalysts because of their ability to catalyze a wide variety of reactions in aqueous and non-aqueous media. The chemo-, regio- and enantio-specific behaviour of these enzymes has caused tremendous interest among scientists and industrialists. Lipases from a large number of bacterial, fungal and a few plant and animal sources have been purified to homogeneity. This article presents a critical review of different strategies which have been employed for the detection, purification and characterization of microbial lipases.
Hasan
Fariha
F
Department of Microbiology, Quaid-i-Azam University, Islamabad, Pakistan.
Shah
Aamer Ali
AA
Department of Microbiology, Quaid-i-Azam University, Islamabad, Pakistan. Electronic address: alishah_75@yahoo.com.
Hameed
Abdul
A
Department of Microbiology, Quaid-i-Azam University, Islamabad, Pakistan.
D055162:Biocatalysis; D008049:Lipase / Q000096:biosynthesis / Q000302:isolation & purification / Q000378:metabolism*; D008066:Lipolysis
D008049:Lipase