title: Three-dimensional structure of the two-peptide bacteriocin plantaricin JK.
abstract: The three-dimensional structures of the two peptides, PlnJ and PlnK, that constitutes the two-peptide bacteriocin plantaricin JK have been solved in water/TFE and water/DPC-micellar solutions using nuclear magnetic resonance (NMR) spectroscopy. PlnJ, a 25 residue peptide, has an N-terminal amphiphilic alpha-helix between Trp-3 and Tyr-15. The 32 residues long PlnK forms a central amphiphilic alpha-helix between Gly-9 and Leu-24. Measurements of the effect on anti-microbial activity of single glycine replacements in PlnJ and PlnK show that Gly-13 and Gly-17 in both peptides are very sensitive, giving more than a 100-fold reduction in activity when large residues replace glycine. In variants where other glycine residues, Gly-20 in PlnJ and Gly-7, Gly-9, Gly-24 and Gly-25 in PlnK, were replaced, the activity was reduced less than 10-fold. It is proposed that the detrimental effect on activity when exchanging Gly-13 and Gly-17 in PlnJ and PlnK is a result of reduced ability of the two peptides to interact through the GxxxG-motifs constituting Gly-13 and Gly-17.
authors.affiliation: Department of Molecular Biosciences, University of Oslo, Blindern, Oslo, Norway. p.a.rogne@imbv.uio.no
authors.forename: Per
authors.initials: P
authors.lastname: Rogne
authors.forename: Christofer
authors.initials: C
authors.lastname: Haugen
authors.forename: Gunnar
authors.initials: G
authors.lastname: Fimland
authors.forename: Jon
authors.initials: J
authors.lastname: Nissen-Meyer
authors.forename: Per Eugen
authors.initials: PE
authors.lastname: Kristiansen
mesh_terms: D000595:Amino Acid Sequence / Q000235:genetics; D019943:Amino Acid Substitution / Q000235:genetics; D001430:Bacteriocins / Q000096:biosynthesis / Q000737:chemistry* / Q000493:pharmacokinetics / Q000494:pharmacology; D049109:Cell Proliferation / Q000187:drug effects; D002942:Circular Dichroism; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D005998:Glycine / Q000235:genetics; D007778:Lactobacillus / Q000187:drug effects; D048191:Lactiplantibacillus plantarum / Q000187:drug effects / Q000235:genetics; D008081:Liposomes / Q000737:chemistry; D008823:Micelles; D008958:Models, Molecular; D009587:Nitrogen Isotopes / Q000378:metabolism; D019906:Nuclear Magnetic Resonance, Biomolecular; D010767:Phosphorylcholine / Q000031:analogs & derivatives / Q000737:chemistry; D017434:Protein Structure, Tertiary; D021122:Protein Subunits / Q000096:biosynthesis / Q000737:chemistry / Q000494:pharmacology; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000494:pharmacology; D014270:Trifluoroethanol / Q000737:chemistry
chemical_list: D001430:Bacteriocins; D008081:Liposomes; D008823:Micelles; D009587:Nitrogen Isotopes; D021122:Protein Subunits; D011994:Recombinant Proteins; C117245:plantaricin JK; D010767:Phosphorylcholine; C028810:dodecylphosphocholine; D014270:Trifluoroethanol; D005998:Glycine