Expressions of eNOS3 and Ve-cadherin in microvascular endothelium at 7-day of reperfused acute myocardial infarction.
OBJECTIVE
To investigate the expressions of eNOS3 and Ve-cadherin at the first week of reperfused acute myocardial infarction (AMI).


METHODS
16 of mini-swines (20 to 30 Kg) were randomly assigned to the sham-operated group and the AMI group. Pathologic myocardial tissue was collected at 7-day of LAD reperfusion and assessed by immunofluorescence and laser co-focus microscope scan, in-situ hybridization, real-time quantitative polymerase chain reaction and western blot.


RESULTS
At 7-day of reperfusion, the eNOS3 mRNA and protein expressions in the infarcted and marginal areas were lower than those in the normal area and sham-operated area (all P<0.05), similarly the infarcted and marginal areas had lower Ve-cadherin mRNA expression than the normal area and the sham-operated area (all P<0.05), and Ve-cadherin protein expression was lower in the infarcted area compared with the marginal area, the normal area and the sham-operated area (all P<0.05).


CONCLUSIONS
Low expressions of eNOS3 and Ve-cadherin in the salvaged sub-healthy microvascular endothelium of infarcted and marginal areas suggest that endothelial system is impaired at 7-day of reperfused acute myocardial infarction.
Kang
Sheng
S
Center of Cardiology, Shanghai East Hospital, Tongji University, Jimo Rd 150, Shanghai 200120, People's Republic of China. kangsheng2008@163.com
Yang
Yue-jin
YJ
Wang
Qing-zhi
QZ
Li
Yue
Y
Tian
Yi
Y
Cheng
Yu-tong
YT
D000818:Animals; D015703:Antigens, CD / Q000096:biosynthesis* / Q000235:genetics; D015820:Cadherins / Q000096:biosynthesis* / Q000235:genetics; D004730:Endothelium, Vascular / Q000378:metabolism*; D066298:In Vitro Techniques; D055806:Microvessels / Q000378:metabolism*; D009203:Myocardial Infarction / Q000378:metabolism* / Q000473:pathology; D015425:Myocardial Reperfusion; D052250:Nitric Oxide Synthase Type III / Q000096:biosynthesis* / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis; D013552:Swine; D013556:Swine, Miniature; D000099152:Cadherin 5
D015703:Antigens, CD; D015820:Cadherins; D052250:Nitric Oxide Synthase Type III; D012333:RNA, Messenger; D000099152:Cadherin 5