Identification and characterization of a novel oligomeric decaprenyl diphosphate synthase.
A novel oligomeric decaprenyl pyrophosphate synthase was identified. Circular dichroism measurements indicated that it is alpha-helical, and stable against pH changes and denaturants. Three peptides corresponding to the conserved regions were synthesized and metal affinities were investigated. Crystallographic analyses of this oligomeric DDS are currently in progress.
Choe
Hyeong H
HH
Department of Molecular Science and Technology, Graduate School of Interdisciplinary Program, Ajou University, Suwon, South Korea, 443-749.
Hwang
Heejin
H
Kim
SeungBum
S
Yoon
Sangyoung
S
Jo
Do Hyun
DH
Ryu
Yeonwoo
Y
Kim
T Doohun
TD
D016960:Agrobacterium tumefaciens / Q000201:enzymology*; D019883:Alkyl and Aryl Transferases / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D000595:Amino Acid Sequence; D017124:Conserved Sequence; D008670:Metals / Q000378:metabolism
D008670:Metals; D019883:Alkyl and Aryl Transferases; C039821:decaprenyl pyrophosphate synthetase