7-ketocholesterol induces endoplasmic reticulum stress in HT-29 cells.
7-Ketocholesterol (7-Kchol, oxidized cholesterol) is an important mediator of cell death in atherosclerosis mediated by up-regulated Nox 4 gene expression. In the current study using the human colon cancer HT-29 cell line, we have demonstrated that 7-Kchol promotes endoplasmic reticulum (ER) stress via gene up-regulation of ER chaperone and membrane kinases.
Lee
Won-Hyo
WH
Department of Pathology, Chungnam National University, College of Medicine, Taejon 301-747, Korea.
Lee
Choong Sik
CS
Kwon
Kisang
K
Kwon
Young-Sook
YS
Kim
Seung-Whan
SW
Goo
Tae-Won
TW
Kwon
O-Yu
OY
D045744:Cell Line, Tumor; D003110:Colonic Neoplasms; D017931:DNA Primers; D004721:Endoplasmic Reticulum / Q000187:drug effects / Q000502:physiology*; D019073:HT29 Cells / Q000187:drug effects / Q000502:physiology*; D006801:Humans; D007653:Ketocholesterols / Q000494:pharmacology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction / Q000187:drug effects
D017931:DNA Primers; D007653:Ketocholesterols; C003001:7-ketocholesterol