Monitoring of glucose and glutamate using enzyme microstructures and scanning electrochemical microscopy.
Glucose oxidase and glutamate oxidase lines, with typical width of 100 microm, were patterned on gold surfaces using a micro-dispensing system, by shooting 100 pl droplets of the corresponding enzyme solutions. The probe of a scanning electrochemical microscope (SECM) was then carefully positioned in the close proximity of the enzyme microstructure and poised to +600 mV vs. Ag/AgCl, KCl 0.1 M. The H(2)O(2), generated by the enzyme lines at different concentrations of glucose and glutamate in the surrounding solution, was sequentially monitored. Reproducible calibration curves for glucose and glutamate were obtained in one single experiment, proving that the combination of enzyme microstructures with SECM can provide a new way of achieving multianalyte detection.
Mureşan
Laura
L
Department of Analytical Chemistry, Lund University, P.O. Box 124, SE-22100 Lund, Sweden. lamur@chem.ubbcluj.ro
Nistor
Mihaela
M
Gáspár
Szilveszter
S
Popescu
Ionel Cătălin
IC
Csöregi
Elisabeth
E
D001234:Aspergillus niger / Q000201:enzymology; D015374:Biosensing Techniques / Q000379:methods*; D002138:Calibration; D004563:Electrochemistry; D005947:Glucose / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D005949:Glucose Oxidase / Q000737:chemistry* / Q000378:metabolism; D018698:Glutamic Acid / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D008853:Microscopy; D015203:Reproducibility of Results
D018698:Glutamic Acid; D005949:Glucose Oxidase; D005947:Glucose