Comparative studies and identification of camptothecin produced by an endophyte at shake flask and bioreactor.
The fungus showing homology with Nodulisporium by 28S ribosomal gene sequencing, which has been discovered as an endophyte on medicinal plant Nothapodytes foetida, was found to produce 45 and 5.5 microg of camptothecin (CPT) per gram of mycelia at bioreactor and at shake flask, respectively, which was further quantified and characterised by various spectroscopic analyses.
Rehman
Suriya
S
Indian Institute of Integrative Medicine (CSIR), Sanatnagar, Srinagar, India. suriyamir@yahoo.com
Shawl
A S
AS
Kour
Amardeep
A
Sultan
Phalisteen
P
Ahmad
Khurheed
K
Khajuria
Ravi
R
Qazi
G N
GN
D000972:Antineoplastic Agents, Phytogenic / Q000096:biosynthesis* / Q000302:isolation & purification; D001203:Ascomycota / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification / Q000378:metabolism*; D019149:Bioreactors; D002166:Camptothecin / Q000096:biosynthesis* / Q000302:isolation & purification; D004271:DNA, Fungal / Q000235:genetics; D005285:Fermentation; D010946:Plants, Medicinal / Q000382:microbiology
D000972:Antineoplastic Agents, Phytogenic; D004271:DNA, Fungal; D002166:Camptothecin