title: PHA(MCL) biosynthesis systems in Pseudomonas aeruginosa and Pseudomonas putida strains show differences on monomer specificities.
abstract: The production of PHA from plant oils by Pseudomonas species soil isolated from a sugarcane crop was evaluated. Out of 22 bacterial strains three were able to use efficiently plant oils to grow and to accumulate PHA. Pseudomonas putida and Pseudomonas aeruginosa strains produced PHA presenting differences on monomer composition compatible with variability on monomer specificity of their PHA biosynthesis system. The molar fraction of 3-hydroxydodecanoate detected in the PHA was linearly correlated to the oleic acid supplied. A non-linear relationship between the molar fractions of 3-hydroxy-6-dodecenoate (3HDdDelta(6)) detected in PHA and the linoleic acid supplied was observed, compatible with saturation in the biosynthesis system capability to channel intermediate of beta-oxidation to PHA synthesis. Although P. putida showed a higher 3HDdDelta(6) yield from linoleic acid when compared to P. aeruginosa, in both species it was less than 10% of the maximum theoretical value. These results contribute to the knowledge about the biosynthesis of PHA with a controlled composition from plant oils allowing in the future establishing the production of these polyesters as tailor-made polymers.
authors.affiliation: Programa de Pós-Graduação Interunidades em Biotecnologia USP, IPT, Instituto Butantan, SP, Brazil.
authors.forename: S R
authors.initials: SR
authors.lastname: Silva-Queiroz
authors.forename: L F
authors.initials: LF
authors.lastname: Silva
authors.forename: J G C
authors.initials: JG
authors.lastname: Pradella
authors.forename: E M
authors.initials: EM
authors.lastname: Pereira
authors.forename: J G C
authors.initials: JG
authors.lastname: Gomez
mesh_terms: D002849:Chromatography, Gas; D019787:Linoleic Acid / Q000378:metabolism; D013058:Mass Spectrometry; D019301:Oleic Acid / Q000378:metabolism; D010938:Plant Oils / Q000378:metabolism; D054813:Polyhydroxyalkanoates / Q000032:analysis / Q000096:biosynthesis*; D011550:Pseudomonas aeruginosa / Q000378:metabolism*; D016958:Pseudomonas putida / Q000378:metabolism*; D013024:Soybean Oil / Q000378:metabolism; D014280:Triglycerides / Q000378:metabolism
chemical_list: D010938:Plant Oils; D054813:Polyhydroxyalkanoates; D014280:Triglycerides; D019301:Oleic Acid; D013024:Soybean Oil; D019787:Linoleic Acid