[Label-free quantitative analysis of proteins using mass-spectrometry].
In the present paper we highlight the dependence of normalized mass spectrometry intensity on protein concentration. We have defined dynamic range of concentrations from 1 nM to 1 mcM. It has been shown that the dependence holds linearity along the whole concentration range as for pure proteins as for proteins mixtures. Our observation revealed that the correlation curve possess protein-specific tangent between 1 nM and 1 mcM. The efficacy of the method evaluated by AQUA and 18O-labelling techniques of quantitative proteomics.
Kopylov
A T
AT
Zgoda
V G
VG
Archakov
A I
AI
D000818:Animals; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods*; D011506:Proteins / Q000032:analysis* / Q000737:chemistry; D040901:Proteomics / Q000379:methods*; D012680:Sensitivity and Specificity
D011506:Proteins