Characterization of cationic liposomes having IL-2 expressed on their external surface, and their affinity to cervical cancer cells expressing the IL-2 receptor.
Anionic, cationic, and neutral liposomes were constructed to contain IL-2 in order to evaluate their affinity to a cervical cancer cell line (INBL) and to determine whether they can present IL-2 on their external surface. When these liposomes were co-cultured with INBL, the anionic liposomes were the only ones found to be cytotoxic. When neutral and cationic liposomes were constructed in the presence of IL-2, IL-2 was detected only on the surface of cationic liposomes by using a fluorescent anti-IL-2 antibody. By co-culturing INBL with IL-2-containing cationic liposomes, and by using fluorescent anti-IL-2 antibody, we found a strong IL-2 presence on the cell membranes thus suggesting a high affinity of the liposomes to the INBL cells.
Corona-Ortega
Teresa
T
FES-Zaragoza, Universidad Nacional Autónoma de México, Mexico City, Mexico. tcvaldes@servidor.unam.mx
Rangel-Corona
Rosalva
R
Hernández-Jiménez
Miriam
M
Baeza
Isabel
I
Ibáñez
Miguel
M
Weiss-Steider
Benny
B
D000838:Anions; D000970:Antineoplastic Agents / Q000008:administration & dosage*; D002412:Cations; D045744:Cell Line, Tumor; D002784:Cholesterol / Q000737:chemistry; D016503:Drug Delivery Systems; D005260:Female; D005455:Fluorescent Antibody Technique; D006801:Humans; D007376:Interleukin-2 / Q000008:administration & dosage*; D008081:Liposomes; D015375:Receptors, Interleukin-2 / Q000235:genetics*; D013095:Spermidine / Q000737:chemistry; D002583:Uterine Cervical Neoplasms / Q000235:genetics / Q000378:metabolism
D000838:Anions; D000970:Antineoplastic Agents; D002412:Cations; D007376:Interleukin-2; D008081:Liposomes; D015375:Receptors, Interleukin-2; D002784:Cholesterol; D013095:Spermidine