CDK11p58 represses vitamin D receptor-mediated transcriptional activation through promoting its ubiquitin-proteasome degradation.
Vitamin D receptor (VDR) is a member of the nuclear receptor superfamily and regulates transcription of target genes. In this study, we identified CDK11(p58) as a novel protein involved in the regulation of VDR. CDK11(p58), a member of the large family of p34cdc2-related kinases, is associated with cell cycle progression, tumorigenesis, and apoptotic signaling. Our study demonstrated that CDK11(p58) interacted with VDR and repressed VDR-dependent transcriptional activation. Furthermore, overexpression of CDK11(p58) decreased the stability of VDR through promoting its ubiquitin-proteasome-mediated degradation. Taken together, these results suggest that CDK11(p58) is involved in the negative regulation of VDR.
Chi
Yayun
Y
Gene Research Center, Shanghai Medical College and Institutes of Biomedical, Shanghai 200032, People's Republic of China.
Hong
Yi
Y
Zong
Hongliang
H
Wang
Yanlin
Y
Zou
Weiying
W
Yang
Junwu
J
Kong
Xiangfei
X
Yun
Xiaojing
X
Gu
Jianxin
J
D000818:Animals; D019556:COS Cells; D002522:Chlorocebus aethiops; D056743:Cyclin D3; D016213:Cyclins / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D046988:Proteasome Endopeptidase Complex / Q000378:metabolism*; D018167:Receptors, Calcitriol / Q000235:genetics / Q000378:metabolism*; D015533:Transcriptional Activation; D014162:Transfection; D025801:Ubiquitin / Q000378:metabolism; D054875:Ubiquitination
C534696:CCND3 protein, human; D056743:Cyclin D3; D016213:Cyclins; D018167:Receptors, Calcitriol; D025801:Ubiquitin; D046988:Proteasome Endopeptidase Complex