title: Studying the proliferation of human peripheral blood T lymphocytes in serum-free medium.
abstract: We compared the cultivation of human peripheral blood lymphocytes in serum-free medium Hybris-2 and RPMI 1640 medium with 10% fetal bovine serum in the presence of phytohemagglutinin and interleukin-2. The optimal concentration of phytohemagglutinin significantly differed in serum-free and serum-containing media (0.5 and 5 microg/ml, [corrected] respectively). Both mitogens were more potent in stimulating the proliferation of lymphocytes in serum-free medium than in serum-containing medium. Strong proliferation of CD3(+) and CD4(+) T lymphocytes was observed in both media. The dynamics of other markers was similar in serum-free and serum-containing media. However, significant differences were revealed between individual donors. Our results indicate that the developed serum-free medium may be used in lymphocyte cultivation for scientific, diagnostic, and therapeutic purposes.
authors.affiliation: Medical Genetic Research Center, Russian Academy of Medical Sciences, Moscow, Russia. biovitr@rambler.ru
authors.forename: V U
authors.initials: VU
authors.lastname: Tabakov
authors.forename: M M
authors.initials: MM
authors.lastname: Litvina
authors.forename: J V
authors.initials: JV
authors.lastname: Schepkina
authors.forename: A A
authors.initials: AA
authors.lastname: Jarilin
authors.forename: V V
authors.initials: VV
authors.lastname: Chestkov
mesh_terms: D017252:CD3 Complex / Q000276:immunology; D015704:CD4 Antigens / Q000276:immunology; D049109:Cell Proliferation; D002478:Cells, Cultured; D016895:Culture Media, Serum-Free; D006801:Humans; D007377:Interleukin-3 / Q000276:immunology; D010835:Phytohemagglutinins; D013601:T-Lymphocytes / Q000166:cytology* / Q000276:immunology
chemical_list: D017252:CD3 Complex; D015704:CD4 Antigens; D016895:Culture Media, Serum-Free; D007377:Interleukin-3; D010835:Phytohemagglutinins