title: Effect of alcohol consumption on the liver detoxication capacity as measured by [13C2]aminopyrine and L-[1-13C]phenylalanine breath tests.
abstract: The aim of this study was to investigate the hepatic microsomal and cytosolic functions by using the 13CO2 breath test in healthy subjects either before or after consumption of red wine. Twelve adults received [13C2]aminopyrine and L-[1-13C]phenylalanine together with a standardised dinner. Expired air samples were taken over 6 h. After a wash-out period, the subjects consumed 0.4 ml ethanol per kg per day together with dinner over a 7.5-day period on average. Thereafter, 13C-tracer administration was repeated under identical conditions. The 13CO2 enrichments were measured by isotope ratio mass spectrometry. The mean cumulative percentage 13C-dose recovery after administration of [13C2]aminopyrine and L-[1-13C]phenylalanine either without or with red wine consumption amounted to 17.0+/-4.4 vs. 14.7+/-3.1% (p=0.170) and 14.0+/-2.8 vs. 11.5+/-3.9% (p=0.084), respectively. Moderate alcohol consumption does not induce significant short-term changes of the microsomal and the cytosolic function of the human liver in healthy subjects.
authors.affiliation: Children's Hospital, Research Laboratory 'Gastroenterology and Nutrition', University of Rostock, Rostock, Germany. klaus-dieter.wutzke@med.uni-rostock.de
authors.forename: Klaus D
authors.initials: KD
authors.lastname: Wutzke
authors.forename: Marianne
authors.initials: M
authors.lastname: Wigger
mesh_terms: D000328:Adult; D000632:Aminopyrine / Q000032:analysis* / Q000378:metabolism; D001944:Breath Tests; D002245:Carbon Dioxide / Q000032:analysis; D002247:Carbon Isotopes; D018592:Cross-Over Studies; D004305:Dose-Response Relationship, Drug; D000431:Ethanol / Q000494:pharmacology*; D005260:Female; D006801:Humans; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D008862:Microsomes, Liver / Q000187:drug effects / Q000378:metabolism; D010649:Phenylalanine / Q000032:analysis* / Q000378:metabolism; D014920:Wine; D055815:Young Adult
chemical_list: D002247:Carbon Isotopes; D000632:Aminopyrine; D002245:Carbon Dioxide; D000431:Ethanol; D010649:Phenylalanine