Estradiol modulates uterine 18 kDa translocator protein gene expression in uterus and kidney of rats.
We examined the effect of ovariectomy, with and without estradiol treatment, on 18 kDa translocator protein (TSPO) gene expression and its binding density in the uterus and kidney of rats. Ovariectomy causes a significant decrease in uterine, but not renal TSPO binding density, while estradiol treatment of ovariectomized rats restored TSPO binding density in the uterus. These TSPO density levels did not correlate with steady state or new RNA transcription. Our in vivo study suggests that estradiol is responsible for the maintenance of uterine TSPO density via transcriptional mechanisms. Our in vivo study also suggests that in the kidney estradiol appears to operate via post-transcriptional mechanisms to maintain TSPO density.
Mazurika
Caroline
C
Department of Molecular Pharmacology, the Bruce Rappaport Faculty of Medicine, Technion, Haifa, Israel.
Veenman
Leo
L
Weizman
Ronit
R
Bidder
Miri
M
Leschiner
Svetlana
S
Golani
Idit
I
Spanier
Ilana
I
Weisinger
Gary
G
Gavish
Moshe
M
D000818:Animals; D002352:Carrier Proteins / Q000235:genetics*; D004958:Estradiol / Q000494:pharmacology*; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects*; D007668:Kidney / Q000187:drug effects* / Q000378:metabolism*; D008970:Molecular Weight; D010052:Ovariectomy; D011485:Protein Binding / Q000187:drug effects; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D011963:Receptors, GABA-A / Q000235:genetics*; D014158:Transcription, Genetic / Q000187:drug effects; D014599:Uterus / Q000187:drug effects* / Q000378:metabolism*
D002352:Carrier Proteins; D012333:RNA, Messenger; D011963:Receptors, GABA-A; C069997:Tspo protein, rat; D004958:Estradiol