title: Biological effects of brachytherapy using a (32)P-patch on the skin of Sencar mice.
abstract: In recent years, specially designed patches containing beta emitters have been developed for contact brachytherapy of skin lesions. The aim of the present work was to evaluate the biological effects of the (32)P-patch on the skin of Sencar mice as a result of a brachytherapy treatment. For this purpose, a (32)P-patch was prepared with Chromic (32)P-phosphate and silicone and the classical model of two-stage skin carcinogenesis was reproduced in Sencar mice. Animals were divided in six groups. Four groups received the contact brachytherapy treatments using a scheme of a single session of 40 and 60Gy (SD40 and SD60) and a scheme of two sessions of 40 and 60Gy each (FD40 and FD60). The other two groups were used as controls of the single (CSD) and the fractionated (CFD) treatments. Radiation doses were estimated with equations derived from the MIRD DOSE scheme, and biologically effective doses (BED) were calculated according to equations derived from the linear-quadratic model. The endpoint to evaluate the treatments effects was tumor size after a follow-up period of 44 days. Finally, animals were sacrificed in order to get samples of all tumors for histological analysis and PCNA staining. Erythema, dermatitis and skin ulceration developed in almost all treated animals, but they gradually healed with regeneration of tissue during the follow-up period. Radiation effects on the skin of SD40, SD60, FD40 and FD60 showed a significant reduction of the tumor size with regard to controls, independently of the scheme and the radiation dose considered. PCNA staining scores of control groups were higher than for treated groups, independently of the scheme and the radiation dose considered. This radioactive (32)P-silicone-patch which is easy to prepare and use in the treatment of skin diseases, seems promising as a radioactive device for clinical use.
authors.affiliation: Radioisotope Laboratory, School of Pharmacy and Biochemistry, University of Buenos Aires, Buenos Aires, Argentina. jsalgueiro@ffyb.uba.ar
authors.forename: M J
authors.initials: MJ
authors.lastname: Salgueiro
authors.forename: N
authors.initials: N
authors.lastname: Collia
authors.forename: H
authors.initials: H
authors.lastname: Durán
authors.forename: M
authors.initials: M
authors.lastname: Palmieri
authors.forename: V
authors.initials: V
authors.lastname: Medina
authors.forename: R
authors.initials: R
authors.lastname: Ughetti
authors.forename: J
authors.initials: J
authors.lastname: Nicolini
authors.forename: M
authors.initials: M
authors.lastname: Zubillaga
mesh_terms: D000279:Administration, Cutaneous; D000818:Animals; D001918:Brachytherapy / Q000379:methods*; D005260:Female; D051379:Mice; D018632:Mice, Inbred SENCAR; D010761:Phosphorus Radioisotopes / Q000627:therapeutic use*; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism; D011879:Radiotherapy Dosage; D012867:Skin / Q000528:radiation effects*; D012878:Skin Neoplasms / Q000378:metabolism / Q000532:radiotherapy*
chemical_list: D010761:Phosphorus Radioisotopes; D018809:Proliferating Cell Nuclear Antigen