title: Dual functional molecular imaging probe targeting CD20 with PET and optical imaging.
abstract: The present study aimed to develop a monoclonal antibody (mAb)-based double functional probe for PET and near-infrared fluorescence (NIRF) targeting CD20 and to cross validate the targeting efficacy of this dual functional probe. NuB2, anti CD20 mAb was conjugated with Alexa Fluor 750 and 64Cu through DOTA chelator. PET and NIRF imaging was carried out at 30 min and 24 h post-injection with 64Cu-DOTA-NuB2-Alexa Fluor 750 in CD20 positive Raji lymphoma-bearing mice. Fluorescence intensity and radio-activity were studied by ex vivo biodistribution study at 30 min, 24 and 48 h after injection. Raji tumor showed significantly higher uptake of DOTA-NuB2-Alexa Fluor 750 than that of DOTA-Alexa Fluor 750 (p<0.05). Significant correlation was obtained between the organ-to-muscle ratios measured by the radioactivity and fluorescence intensity (p<2.2e-16, r=0.94). Our findings demonstrate the effectiveness and feasibility of preparing an effective mAb-based dual functional imaging agent for PET and NIRF targeting the CD20 expression in lymphoma.
authors.affiliation: Department of Diagnostic Radiology and Nuclear Medicine, Gunma University Graduate School of Medicine, Maebashi, Gunma, Japan. pramila@med.gunma-u.ac.jp
authors.forename: Pramila
authors.initials: P
authors.lastname: Paudyal
authors.forename: Bishnuhari
authors.initials: B
authors.lastname: Paudyal
authors.forename: Yasuhiko
authors.initials: Y
authors.lastname: Iida
authors.forename: Noboru
authors.initials: N
authors.lastname: Oriuchi
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Hanaoka
authors.forename: Hideyuki
authors.initials: H
authors.lastname: Tominaga
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Ishikita
authors.forename: Hiroki
authors.initials: H
authors.lastname: Yoshioka
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Higuchi
authors.forename: Keigo
authors.initials: K
authors.lastname: Endo
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D018951:Antigens, CD20 / Q000276:immunology*; D045744:Cell Line, Tumor; D002614:Chelating Agents; D003301:Copper Radioisotopes; D005240:Feasibility Studies; D005260:Female; D005456:Fluorescent Dyes; D006573:Heterocyclic Compounds, 1-Ring; D006801:Humans; D008223:Lymphoma / Q000175:diagnosis* / Q000000981:diagnostic imaging / Q000276:immunology / Q000473:pathology; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D015336:Molecular Probe Techniques; D049268:Positron-Emission Tomography; D015203:Reproducibility of Results; D019265:Spectroscopy, Near-Infrared; D013388:Succinimides; D013997:Time Factors
chemical_list: C502599:Alexa Fluor 750; D000911:Antibodies, Monoclonal; D018951:Antigens, CD20; D002614:Chelating Agents; D003301:Copper Radioisotopes; D005456:Fluorescent Dyes; D006573:Heterocyclic Compounds, 1-Ring; D013388:Succinimides; C071349:1,4,7,10-tetraazacyclododecane- 1,4,7,10-tetraacetic acid