title: Expression of angiotensin II type 1 (AT1) and angiotensin II type 2 (AT2) receptors in human granulosa-lutein (GL) cells: correlation with infertility diagnoses.
abstract: OBJECTIVE
  To correlate angiotensin II (AngII) receptor expression by granulosa-lutein (GL) cells from gonadotropin-stimulated follicles with infertility diagnosis and IVF parameters.
  
  
  METHODS
  The mRNA of angiotensin receptors type 1 (AT1) and type 2 (AT2) was studied in aspirated GL cells.
  
  
  METHODS
  University laboratory and private IVF center.
  
  
  METHODS
  Seventy-three IVF patients.
  
  
  METHODS
  Reverse-transcription polymerase chain reaction analysis for relative expression of AT1 and AT2 receptor mRNA in women with no ovarian factor (NOF), poor ovarian reserve (PR), endometriosis (ENDO), and polycystic ovary syndrome (PCOS).
  
  
  METHODS
  Expression of AT1 and AT2 receptor mRNA.
  
  
  RESULTS
  There was a constant approximately 7:1 ratio between AT1 and AT2 receptors and a negative correlation between the AT1/AT2 ratio and patient age. There were statistically significant differences in AngII receptors in individual conditions: NOF showed a correlation between AT1 and AT2 receptors and a negative correlation between AT1 receptor expression, embryo fragmentation and number of metaphase II (MII) oocytes; PR showed a negative correlation between AT2 receptor expression and number of MII oocytes; PCOS AT1 receptor expression correlated negatively with the units of FSH administered and with patients' age; ENDO showed no significant correlations.
  
  
  CONCLUSIONS
  Mural GL cells express AT1 receptor much more than AT2 receptor. AngII receptor expression varies with age and infertility diagnosis. Low expression of AngII receptors was associated with high-dose stimulation in women with PR. Embryo fragmentation in NOF is associated with decreased AT1 receptor expression, supporting a role for AngII in GL cell apoptosis.
authors.affiliation: Laboratorio de Biología del Desarrollo, Departamento de Bioquímica y Biología Molecular, Universidad de La Laguna, La Laguna, Tenerife, Spain.
authors.forename: Oscar
authors.initials: O
authors.lastname: Peña
authors.forename: Angela
authors.initials: A
authors.lastname: Palumbo
authors.forename: Rebeca
authors.initials: R
authors.lastname: González-Fernández
authors.forename: Jairo
authors.initials: J
authors.lastname: Hernández
authors.forename: Frederick
authors.initials: F
authors.lastname: Naftolin
authors.forename: Julio
authors.initials: J
authors.lastname: Avila
mesh_terms: D000328:Adult; D000367:Age Factors; D017209:Apoptosis; D046149:Embryo Culture Techniques; D020964:Embryo Loss; D004715:Endometriosis / Q000175:diagnosis / Q000235:genetics* / Q000628:therapy; D005260:Female; D015571:Follicular Fluid / Q000166:cytology; D018507:Gene Expression Regulation, Developmental; D006801:Humans; D007247:Infertility, Female / Q000175:diagnosis / Q000235:genetics* / Q000628:therapy; D008184:Luteal Cells / Q000378:metabolism* / Q000473:pathology; D008677:Metaphase; D011085:Polycystic Ovary Syndrome / Q000175:diagnosis / Q000235:genetics* / Q000628:therapy; D012333:RNA, Messenger / Q000032:analysis*; D044140:Receptor, Angiotensin, Type 1 / Q000235:genetics* / Q000378:metabolism; D044139:Receptor, Angiotensin, Type 2 / Q000235:genetics* / Q000378:metabolism; D027724:Reproductive Techniques, Assisted; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013997:Time Factors
chemical_list: C544065:AGTR1 protein, human; D012333:RNA, Messenger; D044140:Receptor, Angiotensin, Type 1; D044139:Receptor, Angiotensin, Type 2