title: Synthesis of a novel biodegradable poly(ester amine) (PEAs) copolymer based on low-molecular-weight polyethyleneimine for gene delivery.
abstract: In this paper, a novel biodegradable poly(ester amine) (PEA) copolymer was successfully prepared from low-molecular-weight polyethyleneimine (PEI, Mn=1800) and poly(epsilon-caprolactone)-Pluronic-poly(epsilon-caprolactone) (PCFC) copolymers. According to the results of agarose gel electrophoresis, particle sizes and zeta potential measurement and transfection efficiency, these PEA copolymers showed great ability to condense plasmid DNA effectively into nano-complexes with small particle size (< or =200 nm) and moderate zeta potential (> or =12 mV) at proper polymeric carrier/DNA weight ratio. Compared with low-molecular-PEI (Mn=1800), the obtained PEAs exhibited higher transfection efficiency as well as lower cytotoxicity. These results indicated that such PEAs might have great potential application in gene delivery system.
authors.affiliation: State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, West China Medical School, Sichuan University, Chengdu, 610041, China.
authors.forename: Qingfa
authors.initials: Q
authors.lastname: Guo
authors.forename: Shuai
authors.initials: S
authors.lastname: Shi
authors.forename: Xiuhong
authors.initials: X
authors.lastname: Wang
authors.forename: Bing
authors.initials: B
authors.lastname: Kan
authors.forename: Yingchun
authors.initials: Y
authors.lastname: Gu
authors.forename: Xingyu
authors.initials: X
authors.lastname: Shi
authors.forename: Feng
authors.initials: F
authors.lastname: Luo
authors.forename: Xia
authors.initials: X
authors.lastname: Zhao
authors.forename: Yuquan
authors.initials: Y
authors.lastname: Wei
authors.forename: Zhiyong
authors.initials: Z
authors.lastname: Qian
mesh_terms: D002460:Cell Line; D004247:DNA / Q000008:administration & dosage; D004587:Electrophoresis, Agar Gel; D018014:Gene Transfer Techniques; D006801:Humans; D010316:Particle Size; D010957:Plasmids / Q000008:administration & dosage; D020442:Poloxamer / Q000737:chemistry; D011073:Polyamines / Q000138:chemical synthesis*; D011091:Polyesters / Q000138:chemical synthesis* / Q000737:chemistry; D011094:Polyethyleneimine / Q000737:chemistry; D014162:Transfection
chemical_list: D011073:Polyamines; D011091:Polyesters; D020442:Poloxamer; C016240:polycaprolactone; D011094:Polyethyleneimine; D004247:DNA