title: Two-photon bioimaging with picosecond optical pulses from a semiconductor laser.
abstract: Toward a practical light source for two-photon bioimaging, we have generated kilowatt peak power of 0.77 mum wavelength and 5 ps optical pulse via second-harmonic generation of the amplified output from a gain-switched 1.55 mum semiconductor laser. This compact scheme and stable optical-pulse-source has been successfully used for the two-photon fluorescence bioimaging of actin filaments in PtK2 cells.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Yokoyama
authors.forename: Hengchang
authors.initials: H
authors.lastname: Guo
authors.forename: Takuya
authors.initials: T
authors.lastname: Yoda
authors.forename: Keijiro
authors.initials: K
authors.lastname: Takashima
authors.forename: Ki-Ichi
authors.initials: K
authors.lastname: Sato
authors.forename: Hirokazu
authors.initials: H
authors.lastname: Taniguchi
authors.forename: Hiromasa
authors.initials: H
authors.lastname: Ito