Visualizing intermolecular interactions in T cells.
The use of appropriate fluorescent proteins has allowed the use of FRET microscopy for investigation of intermolecular interactions in living cells. This method has the advantage of both being dynamic and of working at the subcellular level, so that the time and place where proteins interact can be visualized. We have used FRET microscopy to analyze the interactions between the T cell antigen receptor and the coreceptors CD4 and CD8. This chapter reviews data on how these coreceptors are recruited to the immunological synapse, and how they interact when the T cell is stimulated by different ligands.
Gascoigne
Nicholas R J
NR
Department of Immunology and Microbial Science, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA. Gascoigne@scripps.edu
Ampudia
Jeanette
J
Clamme
Jean-Pierre
JP
Fu
Guo
G
Lotz
Carina
C
Mallaun
Michel
M
Niederberger
Nathalie
N
Palmer
Ed
E
Rybakin
Vasily
V
Yachi
Pia P
PP
Zal
Tomasz
T
D000818:Animals; D017951:Antigen Presentation; D015704:CD4 Antigens / Q000378:metabolism*; D016827:CD8 Antigens / Q000378:metabolism*; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods*; D006801:Humans; D043942:Nanotubes; D011948:Receptors, Antigen, T-Cell / Q000378:metabolism*; D013601:T-Lymphocytes / Q000276:immunology* / Q000378:metabolism
D015704:CD4 Antigens; D016827:CD8 Antigens; D011948:Receptors, Antigen, T-Cell