Decreased levels of both Stat1 and Stat3 in T lymphocytes from mice bearing mammary tumors.
BACKGROUND
Lymphocytes from tumor-bearing animals have been shown to lack antitumor function. The objective of this study was to investigate the status of the signal transducers, Stat1 and Stat3, in T lymphocytes of animals bearing D1-DMBA-3 mammary tumors and to elucidate if any alterations in these signal transducers can be explained by the presence of tumor-derived factors and correlated with the lack of antitumor function in these cells.


METHODS
T Lymphocytes from spleens of normal and tumor-bearing mice were purified and assayed for the presence of Stat1 and Stat3 by Western blot analysis.


RESULTS
It was found that levels of both Stat1 and Stat3 were reduced in T lymphocytes of tumor-bearers not only in their active, phosphorylated form but in total protein levels.


CONCLUSIONS
These findings indicate that during mammary tumor progression, alteration of various transcription factors may contribute to the down-regulation of immune function.
Handel-Fernandez
Mary Ellen
ME
Department of Microbiology and Immunology, Miller School of Medicine, University of Miami, Miami, FL 33136, U.S.A.
Ilkovitch
Dan
D
Iragavarapu-Charyulu
Vijaya
V
Herbert
Lynn M
LM
Lopez
Diana M
DM
D015127:9,10-Dimethyl-1,2-benzanthracene / Q000633:toxicity; D000230:Adenocarcinoma / Q000139:chemically induced / Q000276:immunology / Q000378:metabolism*; D000818:Animals; D015153:Blotting, Western; D002273:Carcinogens / Q000633:toxicity; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D008325:Mammary Neoplasms, Experimental / Q000139:chemically induced / Q000276:immunology / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D010766:Phosphorylation; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D050794:STAT1 Transcription Factor / Q000235:genetics / Q000378:metabolism*; D050796:STAT3 Transcription Factor / Q000235:genetics / Q000378:metabolism*; D013601:T-Lymphocytes / Q000378:metabolism*
D002273:Carcinogens; D012333:RNA, Messenger; D050794:STAT1 Transcription Factor; D050796:STAT3 Transcription Factor; C494080:Stat1 protein, mouse; C494087:Stat3 protein, mouse; D015127:9,10-Dimethyl-1,2-benzanthracene