Resonant four-wave mixing of gold nanoparticles for three-dimensional cell microscopy.
By detecting the transient four-wave mixing from gold nanoparticles in resonance with their surface plasmon, we demonstrate a multiphoton imaging modality suited for cell microscopy. Four-wave mixing is measured free from background using a three-beam excitation geometry and heterodyne detection. We achieve a spatial resolution of 140 nm in-plane and 470 nm in the axial direction, surpassing the one-photon diffraction limit. With this technique, high-contrast photostable imaging of Golgi structures is demonstrated in HepG2 cells labeled with gold nanoparticles of 10 nm and 5 nm diameter.
Masia
Francesco
F
School of Biosciences, Cardiff University, Museum Avenue, Cardiff CF10 3AX, United Kingdom.
Langbein
Wolfgang
W
Watson
Peter
P
Borri
Paola
P
D045744:Cell Line, Tumor; D006046:Gold / Q000737:chemistry*; D006056:Golgi Apparatus / Q000648:ultrastructure; D006801:Humans; D021621:Imaging, Three-Dimensional; D053768:Metal Nanoparticles / Q000737:chemistry*; D008853:Microscopy / Q000295:instrumentation / Q000379:methods; D036103:Nanotechnology; D020349:Surface Plasmon Resonance / Q000379:methods
D006046:Gold