title: Protocols for in vitro culture and phytochemical analysis of Phyllanthus species (euphorbiaceae).
abstract: We developed reproducible protocols for micropropagation, callus culture, and root culture of the medicinal plant Phyllanthus urinaria, P. niruri, P. tenellus, P. corcovadensis, P. caroliniensis, P. stipulatus, and P. fraternus by using single node explants. Genotype-linked differences are visible among the Phyllanthus species concerning shoot culture, callus culture, and root culture growth. The protocols developed for phytochemical screening of callus and root extracts of P. urinaria, P. caroliniensis, P. stipulatus, and P. fraternus have shown the production of sterols and triterpenes. Both compounds are known to account for the antinociceptive activity of the methanolic extracts as glochidone and stigmasterol have strong activity against neurogenic and inflammatory pain. Similarly, methanolic callus extracts of P. tenellus, P. niruri and P. corcovadensis have potent analgesic properties, however phenolics are major compounds isolated from these species. The optimized micropropagation, callus culture, and root culture protocols offer the possibility to use cell/root culture techniques for vegetative propagation and secondary metabolite studies.
authors.affiliation: Departamento de Botânica, Universidade Federal de Santa Catarina, Florianópolis, SC, Brasil.
authors.forename: Elizabete
authors.initials: E
authors.lastname: Catapan
authors.forename: Fábio Netto
authors.initials: FN
authors.lastname: Moreno
authors.forename: Márcio
authors.initials: M
authors.lastname: Luís Busi da Silva
authors.forename: Michel Fleith
authors.initials: MF
authors.lastname: Otuki
authors.forename: Rivaldo
authors.initials: R
authors.lastname: Niero
authors.forename: Valdir Cechinel
authors.initials: VC
authors.lastname: Filho
authors.forename: Rosendo
authors.initials: R
authors.lastname: Augusto Yunes
authors.forename: Ana Maria
authors.initials: AM
authors.lastname: Viana
mesh_terms: D002849:Chromatography, Gas; D002851:Chromatography, High Pressure Liquid; D029803:Phyllanthus / Q000737:chemistry* / Q000145:classification / Q000254:growth & development*; D013045:Species Specificity; D046509:Tissue Culture Techniques