title: Apoptotic death mode of mitomycin C-treated HeLa cells and cellular localization of mitomycin C-induced P-glycoprotein.
abstract: Mitomycin C (MMC) is an active antineoplastic agent and is suggested to induce apoptosis in a caspase- dependent manner in human gastric, bladder, and breast cancer cells. In this study, the death mode of human cervical cancer cells (HeLa) induced by MMC and the cellular localization of MMC-induced P-glycoprotein (P-gp) were investigated. The results of caspase-3 activity, Annexin V binding, and DNA fragmentation suggested that the degree of caspase-dependent apoptosis induced by MMC was in a dose-, but not time-dependent, manner. Further, in low-dose (0.0299 microM) and long-term (2 months) treatment with MMC, P-gp is itself extruded from the cells and colocalized with nuclear DNA and the overexpression was achieved.
authors.affiliation: School of Dentistry, Chung Shan Medical University, Taichung, Taiwan, ROC.
authors.forename: Min-Hsiung
authors.initials: MH
authors.lastname: Cheng
authors.forename: Hao-Tsai
authors.initials: HT
authors.lastname: Cheng
authors.forename: Shih-Shen
authors.initials: SS
authors.lastname: Lin
authors.forename: Su-Chung
authors.initials: SC
authors.lastname: Young
authors.forename: Chien-Jung
authors.initials: CJ
authors.lastname: Pai
authors.forename: Pao-Hsin
authors.initials: PH
authors.lastname: Liao
authors.forename: Shu-Chen
authors.initials: SC
authors.lastname: Chen
authors.forename: Ming-Yung
authors.initials: MY
authors.lastname: Chou
authors.forename: Jaw-Ji
authors.initials: JJ
authors.lastname: Yang
authors.forename: Chi-Chiang
authors.initials: CC
authors.lastname: Yang
mesh_terms: D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1 / Q000187:drug effects / Q000378:metabolism*; D017304:Annexin A5 / Q000378:metabolism; D017209:Apoptosis / Q000187:drug effects*; D016923:Cell Death / Q000187:drug effects*; D045744:Cell Line, Tumor; D002478:Cells, Cultured; D053938:DNA Fragmentation / Q000187:drug effects; D006367:HeLa Cells; D006801:Humans; D008297:Male; D016685:Mitomycin / Q000494:pharmacology*
chemical_list: D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D017304:Annexin A5; D016685:Mitomycin