Characterization of in vivo effects of platelet-rich plasma and biodegradable gelatin hydrogel microspheres on degenerated intervertebral discs.
We have previously shown that administration of platelet-rich plasma-impregnated gelatin hydrogel microspheres (PRP-GHMs) into a degenerated intervertebral disc (IVD) markedly suppresses progression of IVD degeneration. In the current study, we characterized the in vivo effects of PRP-GHM treatment in a degenerated IVD model in rabbit. On magnetic resonance images, the IVD height was significantly greater after treatment with PRP-GHMs compared with phosphate-buffered saline-impregnated GHMs, PRP without GHMs, and needle puncture only. Water content was also preserved in PRP-GHM-treated IVDs. Consistent with this observation, the mRNA expression of proteoglycan core protein and type II collagen was significantly higher after PRP-GHM treatment compared with other treatment groups. No proliferating cells were found in the nucleus pulposus and inner annulus fibrosus in any groups, but the number of apoptotic cells in the nucleus pulposus after PRP-GHM treatment was significantly lower than that after other treatments. These results provide an improved understanding of the therapeutic effects of PRP-GHM treatment of degenerated IVDs.
Sawamura
Kazuhide
K
Department of Orthopaedics, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, Kyoto, Japan. sawamura0513@cup.ocn.ne.jp
Ikeda
Takumi
T
Nagae
Masateru
M
Okamoto
Shin-ichi
S
Mikami
Yasuo
Y
Hase
Hitoshi
H
Ikoma
Kazuya
K
Yamada
Tetsuya
T
Sakamoto
Hirotaka
H
Matsuda
Ken-ichi
K
Tabata
Yasuhiko
Y
Kawata
Mitsuhiro
M
Kubo
Toshikazu
T
D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D001672:Biocompatible Materials / Q000494:pharmacology*; D002417:Cattle; D049109:Cell Proliferation / Q000187:drug effects; D024043:Collagen Type II / Q000235:genetics / Q000378:metabolism; D003692:Delayed-Action Preparations / Q000494:pharmacology; D005780:Gelatin / Q000494:pharmacology*; D020869:Gene Expression Profiling; D006801:Humans; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000494:pharmacology*; D020287:In Situ Nick-End Labeling; D007403:Intervertebral Disc / Q000187:drug effects / Q000473:pathology*; D055959:Intervertebral Disc Degeneration / Q000473:pathology / Q000628:therapy*; D008279:Magnetic Resonance Imaging; D008863:Microspheres; D053657:Platelet-Rich Plasma / Q000378:metabolism*; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism; D011509:Proteoglycans / Q000235:genetics / Q000378:metabolism; D011817:Rabbits; D016212:Transforming Growth Factor beta / Q000494:pharmacology
D001672:Biocompatible Materials; D024043:Collagen Type II; D003692:Delayed-Action Preparations; D018809:Proliferating Cell Nuclear Antigen; D011509:Proteoglycans; D016212:Transforming Growth Factor beta; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D005780:Gelatin