Identification of a bovine viral diarrhea virus 2 isolated from cattle in China.
The identification and characterization of bovine viral diarrhea virus 2 (BVDV-2) strain SD-06 isolated from cattle in China is reported. We performed sequence analysis of 5'-untranslated region (5'-UTR) and E2 sequences and the identity at the nucleotide and amino acid level indicated that the isolate was closely related to BVDV-2. The BVDV-2 strain New York'93 showed the highest sequence homology with the isolate SD-06. The phylogenetic analysis revealed that the isolate SD-06 belonged to BVDV-2a subtype. Furthermore, immunofluorescence assay with the monoclonal antibody specific for BVDV-2 glycoprotein E2 confirmed this identification. Thus, the strain SD-06 was the first isolate of BVDV-2 identified in China.
Zhu
L Q
LQ
College of Veterinary Medicine, Yangzhou University, Yangzhou, PR China.
Ren
M
M
Lin
Y Q
YQ
Ding
X Y
XY
Zhang
G P
GP
Zhao
X
X
Zhu
G Q
GQ
D020121:5' Untranslated Regions / Q000235:genetics; D000818:Animals; D001912:Bovine Virus Diarrhea-Mucosal Disease / Q000453:epidemiology / Q000821:virology; D002417:Cattle; D002418:Cattle Diseases / Q000453:epidemiology / Q000821:virology; D002460:Cell Line; D002681:China / Q000453:epidemiology; D030242:Diarrhea Virus 2, Bovine Viral / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D007668:Kidney / Q000166:cytology / Q000821:virology; D008969:Molecular Sequence Data; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D014759:Viral Envelope Proteins / Q000235:genetics*
D020121:5' Untranslated Regions; D014759:Viral Envelope Proteins; C076551:gp53, bovine viral diarrhea virus