[Application of real-time PCR and LightCycler system for investigating the presence of human herpesvirus 7 DNA].
Human herpesvirus 7 (HHV-7) is a beta-herpesvirus widely spread within a population and has been recognized as a potential pathogen in immunocompromised hosts. The goal of the study was development of real-time PCR assay for detection of human herpesvirus 7 DNA in clinical samples, using primers targeting a conserved region of the viral DNA major capside proteine gene and a specific TaqMan hydrolyzing probe. Sixty four plasma samples taken from a group of adult recipients of allogeneic HSCT, during detectable CMV viremia or neutropenic fever, were tested for the presence of viral DNA in the LightCycler system with method described above. HHV-7 DNA was detected in 40 specimens (62.5%). The conclusion is that developed TaqMan-based probes real-time PCR test is very reliable and valuable tool for detection of HHV-7 viremia in plasma samples. The high level of sensitivity and accuracy provided by the LightCycler instrument is favorable for the use of this method in the detection of human herpesvirus 7 DNA in clinical specimens.
Dzieciatkowski
Tomasz
T
Katedra i Zakład Mikrobiologii Lekarskiej, Warszawski Uniwersytet Medyczny. dzieciatkowski@wp.pl
Przybylski
Maciej
M
Kawecki
Dariusz
D
Midak-Siewirska
Anna
A
Gieryńska
Małgorzata
M
Łuczak
Mirosław
M
Młynarczyk
Grazyna
G
D000328:Adult; D001483:Base Sequence; D004279:DNA, Viral / Q000302:isolation & purification*; D016199:Herpesvirus 7, Human / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D019349:Roseolovirus Infections / Q000821:virology*; D014766:Viremia / Q000821:virology*
D004279:DNA, Viral