title: Two-photon fluorescence imaging with a pulse source based on a 980-nm gain-switched laser diode.
abstract: We developed a compact, high-peak-power picosecond pulse source based on a gain-switched laser diode with an emission wavelength of 980 nm and successfully demonstrated two-photon fluorescence imaging. The pulse source consisted of the gain-switched laser diode, a pulse compressor, a pulse reshaper, and optical amplifiers, all connected by single-mode fibers. The optical pulse generated by the pulse source had a pulse width of 3.5 ps, an average power of 35 mW, and kilowatt-level peak power at a repetition rate of 10 MHz. Alexa488-stained Glomeruli and convoluted tubules of a mouse kidney section were observed by two-photon imaging with the developed pulse source.
authors.forename: Kenji
authors.initials: K
authors.lastname: Taira
authors.forename: Takeshi
authors.initials: T
authors.lastname: Hashimoto
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Yokoyama