title: Methylation of HACE1 in gastric carcinoma.
abstract: BACKGROUND
  We recently examined the methylation status of the HACE1 gene in primary carcinomas derived from 32 patients with colorectal cancer. A significant increase was observed in the maximal tumor size of the tumors with methylated HACE1 (p=0.0304). Moreover, a trend was shown toward preferentially developing lymph node metastasis in the carcinomas with methylated HACE1 (p=0.0612), suggesting that HACE1 might present a malignant potential in colorectal cancer. These results prompted us to examine the methylation status of the HACE1 gene in gastric carcinomas.
  
  
  METHODS
  The methylation status of the HACE1 gene was examined in primary carcinomas and the corresponding normal tissues derived from 34 patients with gastric carcinoma using quantitative methylation-specific PCR (qMSP) and the correlation between the methylation status and the clinicopathological findings was evaluated.
  
  
  RESULTS
  An aberrant methylation of the HACE1 gene was detected in 9 out of 34 (26%) primary gastric carcinomas. Subsequently, clinicopathological data were tested for correlation with the methylation score. A significant difference was observed in patient gender (p=0.0429).
  
  
  CONCLUSIONS
  HACE1 was frequently methylated in gastric carcinoma derived from male patients.
authors.affiliation: Department of Surgery, Showa University Fujigaoka Hospital, Aoba-ku, Yokohama 227-8501, Japan.
authors.forename: Makiko
authors.initials: M
authors.lastname: Sakata
authors.forename: Yo-Hei
authors.initials: YH
authors.lastname: Kitamura
authors.forename: Kazuma
authors.initials: K
authors.lastname: Sakuraba
authors.forename: Tetsuhiro
authors.initials: T
authors.lastname: Goto
authors.forename: Hiroki
authors.initials: H
authors.lastname: Mizukami
authors.forename: Mitsuo
authors.initials: M
authors.lastname: Saito
authors.forename: Kazuyoshi
authors.initials: K
authors.lastname: Ishibashi
authors.forename: Gaku
authors.initials: G
authors.lastname: Kigawa
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Nemoto
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Sanada
authors.forename: Kenji
authors.initials: K
authors.lastname: Hibi
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D019175:DNA Methylation; D005260:Female; D005753:Gastric Mucosa / Q000378:metabolism / Q000473:pathology; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008207:Lymphatic Metastasis; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D016133:Polymerase Chain Reaction; D011379:Prognosis; D011401:Promoter Regions, Genetic; D013274:Stomach Neoplasms / Q000235:genetics* / Q000473:pathology*; D044767:Ubiquitin-Protein Ligases / Q000235:genetics*
chemical_list: C497506:HACE1 protein, human; D044767:Ubiquitin-Protein Ligases