title: Interferon-beta modulates protein synthesis in the central nervous system.
abstract: Interferon-beta (IFN-beta), acting canonically via the modulation of transcription, affects neocortical pyramidal neurons. By use of 2-D differential gel electrophoresis and subsequent mass spectrometry we identified IFN-beta regulated proteins in the central nervous system. These proteins are involved in cytoskeleton assembly, protein transport and nucleotide metabolism and, as such, serve regenerative and protective functions. Electrophysiologically, IFN-beta mediated protein synthesis is essential for part of the excitatory neuronal effects, as revealed under blockade of protein biosynthesis. This study presents novel effects of IFN-beta in the central nervous system and begins to unravel the mechanism behind the known excitability changes in neurons.
authors.affiliation: Dept. of Neurology, University of Rostock, Gehlsheimer Str. 20, 18147 Rostock, Germany.
authors.forename: Susanne
authors.initials: S
authors.lastname: Beyer
authors.forename: Gergana
authors.initials: G
authors.lastname: Raether
authors.forename: Konstantin
authors.initials: K
authors.lastname: Stadler
authors.forename: Raimund
authors.initials: R
authors.lastname: Hoffrogge
authors.forename: Christian
authors.initials: C
authors.lastname: Scharf
authors.forename: Arndt
authors.initials: A
authors.lastname: Rolfs
authors.forename: Eilhard
authors.initials: E
authors.lastname: Mix
authors.forename: Ulf
authors.initials: U
authors.lastname: Strauss
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000502:physiology; D002490:Central Nervous System / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D002540:Cerebral Cortex / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D003513:Cycloheximide / Q000494:pharmacology; D003599:Cytoskeleton / Q000187:drug effects / Q000378:metabolism; D015180:Electrophoresis, Gel, Two-Dimensional; D004594:Electrophysiology; D016899:Interferon-beta / Q000378:metabolism / Q000494:pharmacology*; D008297:Male; D013058:Mass Spectrometry; D008564:Membrane Potentials; D009419:Nerve Tissue Proteins / Q000096:biosynthesis / Q000187:drug effects*; D055495:Neurogenesis / Q000187:drug effects / Q000502:physiology; D009474:Neurons / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure; D009924:Organ Culture Techniques; D011500:Protein Synthesis Inhibitors / Q000494:pharmacology; D020543:Proteome / Q000032:analysis / Q000187:drug effects* / Q000378:metabolism; D040901:Proteomics / Q000379:methods; D017966:Pyramidal Cells / Q000187:drug effects / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D015854:Up-Regulation / Q000187:drug effects / Q000502:physiology
chemical_list: D009419:Nerve Tissue Proteins; D011500:Protein Synthesis Inhibitors; D020543:Proteome; D016899:Interferon-beta; D003513:Cycloheximide