Tec kinase mediating IL-8 transcription in monocytes stimulated with LPS.
The purpose of present study was to explore the possibility of Tec kinase as a mediator for IL-8 transcription in monocytes stimulated with LPS. Plasmids of mouse Tec kinase IV or Tec kinase IV with inactivating point mutations generated with QuikChange site-directed mutagenesis were co-transfected with IL-8 promoter driven luciferase construct into RAW264.7 cells, then luciferase activity was measured with a luminometer. The results shown Tec kinase could significantly enhance IL-8 transcription. Furthermore, point inactivating mutation in SH2, PH or PTK domain almost completely abolish the effects of Tec kinase on the transcription of IL-8. In the transfection experiment, PD98059, a MEK1 inhibitor, decreased the transcription of IL-8 in a dose dependent pattern. When siRNA for Tec kinase was transfected into THP-1 cells, it could efficiently block the production of IL-8 from THP-1 cells (p < 0.01) stimulated with LPS. In conclusion, Tec kinase may mediate the transcription of IL-8 in monocyte stimulated with LPS.
Wang
Guang-Qing
GQ
Institute of Burn Surgery, Changhai Hospital, Second Military Medical University, 174 Changhai Road, Shanghai 200433, People's Republic of China.
Yang
Xiao-Yan
XY
Jia
Yi-Tao
YT
Xia
Zhao-Fan
ZF
D000818:Animals; D002460:Cell Line; D016209:Interleukin-8 / Q000096:biosynthesis*; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008264:Macrophages; D051379:Mice; D009000:Monocytes / Q000187:drug effects / Q000378:metabolism*; D017354:Point Mutation; D011401:Promoter Regions, Genetic / Q000235:genetics; D011505:Protein-Tyrosine Kinases / Q000235:genetics / Q000502:physiology*; D014158:Transcription, Genetic
D016209:Interleukin-8; D008070:Lipopolysaccharides; C089848:Tec protein-tyrosine kinase; D011505:Protein-Tyrosine Kinases