title: [Comparing and evaluating six methods of extracting human genomic DNA from whole blood].
abstract: OBJECTIVE
  Comparing the differences in purity and yield among six methods of extracting human genomic DNA from whole blood, which included Classic Phenol-chloroform extraction, modified combined technique composed of improved Phenol-chloroform extraction and Chelex-100 extraction, Chelex-100 extraction, IQ, Qiagen and SP.
  
  
  METHODS
  Ten samples of intravenous whole blood (5 mL/sample) were collected and human genomic DNA was extracted with these six methods. The purity and concentration of the DNA products were detected by ultraviolet spectrophotometry and fluorescent quantitation technique, the yield was calculated and tested with statistical software.
  
  
  RESULTS
  The Chelex-100 extraction was inferior in DNA purity to other methods while the other five methods showed no statistical difference. Modified combined technique was the poorest and IQ was the best in yield among the six methods of extraction. Statistical result showed that the extraction with high quality kits was better than that with classic Phenol-chloroform extraction, Chelex-100 extraction and modified combined technique composed of improved Phenol-chloroform. There was statistical difference between them.
  
  
  CONCLUSIONS
  Comparing to Phenol-chloroform extraction and Chelex-100 extraction, high quality kits are more useful in DNA extraction from forensic materials.
authors.affiliation: Department of Forensic Medicine, Shanghai Medical College, Fudan University, Shanghai 200032, China.
authors.forename: Jing-Jing
authors.initials: JJ
authors.lastname: Chang
authors.forename: Su-Hua
authors.initials: SH
authors.lastname: Zhang
authors.forename: Li
authors.initials: L
authors.lastname: Li
mesh_terms: D002725:Chloroform / Q000737:chemistry*; D004247:DNA / Q000097:blood* / Q000302:isolation & purification*; D005554:Forensic Medicine / Q000379:methods; D023281:Genomics / Q000379:methods*; D006801:Humans; D019800:Phenol / Q000737:chemistry*; D011933:Reagent Kits, Diagnostic; D012117:Resins, Synthetic / Q000737:chemistry
chemical_list: D011933:Reagent Kits, Diagnostic; D012117:Resins, Synthetic; C024997:Chelex 100; D019800:Phenol; D002725:Chloroform; D004247:DNA