title: Mevalonate pathway modulation is associated with hepatitis C virus RNA presence in peripheral blood mononuclear cells.
abstract: Peripheral blood mononuclear cells (PBMC) constitute the main extrahepatic reservoir of hepatitis C virus (HCV). Lipid metabolism of host seems to play important role in HCV infection. The relationship between HCV presence in PBMC and the expression of mevalonate pathway has not been elucidated. The aim of this study was to investigate the association between mevalonate pathway and HCV RNA presence in PBMC after anti-HCV treatment. 67 serum and corresponding PBMC samples were collected from patients at the end of interferon alpha and ribavirin treatment. Serum total cholesterol, HDL-C and LDL-C fractions, triglycerides, as well as intracellular cholesterol and expression level of HMG-CoA reductase, geranylgeranyl pyrophosphate synthase in PBMC were measured and matched for the HCV RNA presence or absence in sera/PBMC. HCV RNA elimination from sera and PBMC was associated with higher serum cholesterol (118.5mg/dL) and LDL-C (66.42mg/dL) levels, compared to the group, where HCV RNA was detected only in PBMC (100.94 and 53.22mg/dL) or the group, where HCV RNA was found in both sera and PBMC (86.79 and 43.79mg/dL) after treatment. Increased expression of geranylgeranyl pyrophosphate synthase was found in the majority of PBMC samples that harbored HCV RNA after elimination of HCV RNA from sera. The expression of mevalonate pathway after antiviral treatment seems to be modulated depending on HCV RNA status in peripheral blood mononuclear cells.
authors.affiliation: Medical University of Lodz, Department of Medical Biochemistry, Lodz, Poland. msidor@zdn.am.lodz.pl
authors.forename: Malgorzata
authors.initials: M
authors.lastname: Sidorkiewicz
authors.forename: Barbara
authors.initials: B
authors.lastname: Józwiak
authors.forename: Barbara
authors.initials: B
authors.lastname: Durys
authors.forename: Ewa
authors.initials: E
authors.lastname: Majda-Stanislawska
authors.forename: Anna
authors.initials: A
authors.lastname: Piekarska
authors.forename: Natalia
authors.initials: N
authors.lastname: Kosciuk
authors.forename: Jadwiga
authors.initials: J
authors.lastname: Ciechowicz
authors.forename: Ewa
authors.initials: E
authors.lastname: Majewska
authors.forename: Jacek
authors.initials: J
authors.lastname: Bartkowiak
mesh_terms: D000293:Adolescent; D000998:Antiviral Agents / Q000627:therapeutic use; D002648:Child; D004359:Drug Therapy, Combination; D005260:Female; D016174:Hepacivirus / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D019698:Hepatitis C, Chronic / Q000188:drug therapy / Q000378:metabolism* / Q000821:virology; D006801:Humans; D000077190:Interferon alpha-2; D016898:Interferon-alpha / Q000627:therapeutic use; D007963:Leukocytes, Mononuclear / Q000187:drug effects / Q000821:virology*; D008297:Male; D053858:Metabolic Networks and Pathways / Q000187:drug effects; D008798:Mevalonic Acid / Q000378:metabolism*; D012367:RNA, Viral / Q000097:blood*; D011994:Recombinant Proteins; D012254:Ribavirin / Q000627:therapeutic use; D055815:Young Adult
chemical_list: D000998:Antiviral Agents; D000077190:Interferon alpha-2; D016898:Interferon-alpha; D012367:RNA, Viral; D011994:Recombinant Proteins; D012254:Ribavirin; D008798:Mevalonic Acid