[Catalytic properties of glutathione reductase from rats liver at norm and toxic hepatitis].
Homogeneous preparations of liver glutathione reductase (GR, EC 1.6.4.2.), obtained from control rats and animals with toxic hepatitis, catalytic properties of this enzyme have been investigated. A number of the enzyme properties did not change under conditions of hepatitis. These included electrophoretic mobility (R(f) = 0.23 +/- 0.01), molecular mass (104 +/- 5.20 kDa), pH-optimum (7.4 +/- 0.37). There was similarity in pK values of functional groups. At the same time, decrease in affinity of enzyme to a substrate and coenzyme and occurrence substrate-linked inhibition was observed under conditions of this pathology. There were some differencies in regulation of GR activity by metabolites of tricarboxylic acid cycle.
Agarkov
A A
AA
Popova
T N
TN
Semenikhina
A V
AV
D000818:Animals; D002384:Catalysis; D056486:Chemical and Drug Induced Liver Injury / Q000201:enzymology*; D002952:Citric Acid Cycle; D005980:Glutathione Reductase / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D006520:Hepatitis, Animal / Q000201:enzymology; D008099:Liver / Q000201:enzymology*; D051381:Rats
D005980:Glutathione Reductase