title: Metabolic profiles of serum from rats after subchronic exposure to chlorpyrifos and carbaryl.
abstract: Chlorpyrifos (CPF) and carbaryl (CAR) have been widely used in agricultural and domestic settings. Previous studies have demonstrated that CPF and CAR are generally neurotoxic to mammals, whereas the toxicities of these pesticides to other organs and their potential interactive effects remain unclear. The purpose of this study assessed the alterations of histopathology, biochemical parameters, and metabolic profiles of serum in rats following the treatment with CPF and CAR alone or in combination. No histopathological changes were observed in the liver and kidney tissues. Biochemical analysis of blood showed that alanine aminotransferase and total bilirubin in serum increased slightly in CPF-treated rats as compared to controls. Metabonomic analysis revealed alternations in a number of metabolites involving the metabolism of glucose, free fatty acids, and amino acids in liver mitochondria. The treatment of rats with CPF alone resulted in a decrease in lactate, low- and very low-density lipoprotein (LDL/VLDL), dimethylglycine (DMG), and aspartate. This was accompanied by an increase in isoleucine and leucine, 3-hydroxybutyrate (3-HB), N-acetylglycoprotein (NAC), acetone, succinate, glutamine, choline, creatine, glucose, and amino acids in a dose-dependent manner. Similarly, treatment with a high dose of CAR alone led to a decrease in DMG, aspartate, LDL/VLDL, and dimethylamine and an increase in taurine, glucose, and amino acids. The levels of lactate and LDL/VLDL decreased, while those of 3-HB, NAC, acetone, succinate, and glutamine elevated in the group of rats treated with a mixture of CPF and CAR as compared to the groups of CPF or CAR alone. Our results suggest that subchronic exposure to CPF and CAR alone, or in combination, could cause a disturbance in energy and fatty acid metabolism in the liver mitochondria of rats. Overall, we have shown that analysis of metabolic profiles can make exceptional contributions to the understanding of the individual or mutual effects following exposure to a low dose of pesticides.
authors.affiliation: Laboratory of Molecular Toxicology, State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, Datunlu Road, Beijing 100101, People's Republic of China.
authors.forename: Hui-Ping
authors.initials: HP
authors.lastname: Wang
authors.forename: Yu-Jie
authors.initials: YJ
authors.lastname: Liang
authors.forename: Ding-Xin
authors.initials: DX
authors.lastname: Long
authors.forename: Jia-Xiang
authors.initials: JX
authors.lastname: Chen
authors.forename: Wei-Yuan
authors.initials: WY
authors.lastname: Hou
authors.forename: Yi-Jun
authors.initials: YJ
authors.lastname: Wu
mesh_terms: D000818:Animals; D012721:Carbaryl / Q000097:blood / Q000378:metabolism / Q000633:toxicity*; D004390:Chlorpyrifos / Q000097:blood / Q000378:metabolism / Q000633:toxicity*; D019963:Clinical Chemistry Tests; D005260:Female; D007306:Insecticides / Q000097:blood / Q000378:metabolism / Q000633:toxicity*; D008099:Liver / Q000473:pathology; D008168:Lung / Q000473:pathology; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D055442:Metabolome / Q000187:drug effects*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D007306:Insecticides; D004390:Chlorpyrifos; D012721:Carbaryl