Detection of cryptic genospecies misidentified as Haemophilus influenzae in routine clinical samples by assessment of marker genes fucK, hap, and sodC.
Clinical isolates of Haemophilus influenzae were assessed for the presence of fucK, hap, and sodC by hybridization with gene-specific probes, and isolates diverging from the expected H. influenzae genotype were characterized by phenotype and 16S rRNA gene sequencing. Two of 480 isolates were finally classified as variant strains ("nonhemolytic Haemophilus haemolyticus").
Nørskov-Lauritsen
Niels
N
Department of Clinical Microbiology, Aarhus University Hospital Skejby, Denmark. nielnoer@rm.dk
D001425:Bacterial Outer Membrane Proteins / Q000235:genetics; D001426:Bacterial Proteins / Q000235:genetics; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics*; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005798:Genes, Bacterial; D006190:Haemophilus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D006192:Haemophilus Infections / Q000382:microbiology*; D006801:Humans; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000235:genetics; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012697:Serine Endopeptidases / Q000235:genetics; D013482:Superoxide Dismutase / Q000235:genetics
D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; C091552:Hap protein, Hemophilus influenzae; D012336:RNA, Ribosomal, 16S; D013482:Superoxide Dismutase; D017853:Phosphotransferases (Alcohol Group Acceptor); D012697:Serine Endopeptidases