Antipyretic and anti-inflammatory properties of the ethanolic extract, dichloromethane fraction and costunolide from Magnolia ovata (Magnoliaceae).
OBJECTIVE
Magnolia ovata (A.St.-Hil.) Spreng (formerly Talauma ovata), known as "pinha-do-brejo" or "baguaçu", is a large tree widely distributed in Brazil. Its trunk bark has been used in folk medicine against fever. However, no data have been published to support the antipyretic ethnopharmacological use. This study investigated the antipyretic and anti-inflammatory effects of the ethanolic extract (EEMO), dichloromethane fraction (DCM), and the isolated compound costunolide.


METHODS
The antipyretic and anti-inflammatory activities were evaluated in experimental models of fever and inflammation in mice.


RESULTS
The oral administration of EEMO, DCM and costunolide inhibited carrageenan (Cg)-induced paw oedema (ID(50) 72.35 (38.64-135.46) mg/kg, 5.8 (2.41-14.04) mg/kg and 0.18 (0.12-0.27) mg/kg, respectively) and was effective in abolishing lipopolysaccharide (LPS)-induced fever (30 mg/kg, 4.5 mg/kg and 0.15 mg/kg, respectively). EEMO was also effective in reducing cell migration in the pleurisy model. Intraplantar injection of costunolide also reduced the paw oedema, myeloperoxidase and N-acetyl-glucosaminidase activity induced by Cg in mice.


CONCLUSIONS
Collectively, these results show, for the first time, that extracts obtained from Magnolia ovata possess antipyretic and anti-inflammatory properties, and costunolide appears to be the compound responsible for these effects.
Kassuya
Cândida Aparecida Leite
CA
Department of Pharmacology, Universidade Federal do Paraná, Curitiba, PR, Brazil.
Cremoneze
Aline
A
Barros
Letícia Ferrari Lemos
LF
Simas
Alex Sandro
AS
Lapa
Fernanda da Rocha
Fda R
Mello-Silva
Renato
R
Stefanello
Maria Elida Alves
ME
Zampronio
Aleksander Roberto
AR
D000118:Acetylglucosaminidase / Q000378:metabolism; D018712:Analgesics, Non-Narcotic / Q000494:pharmacology*; D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology*; D002351:Carrageenan; D002465:Cell Movement / Q000187:drug effects; D004487:Edema / Q000139:chemically induced / Q000517:prevention & control; D000431:Ethanol; D005122:Exudates and Transudates / Q000378:metabolism; D005334:Fever / Q000139:chemically induced / Q000517:prevention & control; D007202:Indicators and Reagents; D007962:Leukocytes / Q000187:drug effects; D008070:Lipopolysaccharides; D031566:Magnolia / Q000737:chemistry*; D008297:Male; D008752:Methylene Chloride; D051379:Mice; D009195:Peroxidase / Q000378:metabolism; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology; D035422:Pleural Cavity / Q000166:cytology; D012717:Sesquiterpenes / Q000737:chemistry / Q000494:pharmacology*; D012997:Solvents; D007783:Lactones
D000118:Acetylglucosaminidase; D018712:Analgesics, Non-Narcotic; D000894:Anti-Inflammatory Agents, Non-Steroidal; D002351:Carrageenan; D000431:Ethanol; D007202:Indicators and Reagents; D008070:Lipopolysaccharides; D008752:Methylene Chloride; D009195:Peroxidase; D010936:Plant Extracts; D012717:Sesquiterpenes; D012997:Solvents; C002602:costunolide; D007783:Lactones