title: Biotin deficiency and biotin excess: effects on the female reproductive system.
abstract: Biotin deficiency and biotin excess have both been found to affect reproduction and cause teratogenic effects. In the reproductive tract, however, the effects of biotin have not been well established yet. We investigated the effects of varying biotin content diets on the oestrus cycle, ovarian morphology, estradiol and progesterone serum levels, and the uterine mRNA abundance of their nuclear receptors, as well as on the activity of the estradiol-degrading group of enzymes cytochrome P450 (CYP) in the liver. Three-week-old female BALB/cAnN Hsd mice were fed a biotin-deficient, a biotin-control, or a biotin-supplemented diet (0, 7.2 or 400 micromol of free biotin/kg diet, respectively) over a period of nine weeks. Striking effects were observed in the biotin-deficient group: mice showed arrested estrous cycle on the day of diestrus and changes in ovary morphology. Estradiol serum concentration increased 49.2% in biotin-deficient mice compared to the control group, while the enzymatic activities of CYP1A2 and CYP2B2 increased (P<0.05). The mRNA abundance of nuclear estrogen and progesterone receptors decreased in the biotin-deficient mice. In the biotin-supplemented group we found that, in spite of a significant (P<0.05) decrease in the number of primary and Graafian follicles and in CYP1A2 activities, mice exhibited 105.4% higher serum estradiol concentration than the control group. No changes in the expression of the nuclear receptors were observed. No significant differences were observed in serum progesterone among the groups. Our results indicate that both the deficiency and the excess of biotin have significant effects on the female mouse reproductive system.
authors.affiliation: Departamento de Biología Celular, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, Mexico.
authors.forename: Armida
authors.initials: A
authors.lastname: Báez-Saldaña
authors.forename: Ignacio
authors.initials: I
authors.lastname: Camacho-Arroyo
authors.forename: J Javier
authors.initials: JJ
authors.lastname: Espinosa-Aguirre
authors.forename: Teresa
authors.initials: T
authors.lastname: Neri-Gómez
authors.forename: Alberto
authors.initials: A
authors.lastname: Rojas-Ochoa
authors.forename: Christian
authors.initials: C
authors.lastname: Guerra-Araiza
authors.forename: Elena
authors.initials: E
authors.lastname: Larrieta
authors.forename: Paz
authors.initials: P
authors.lastname: Vital
authors.forename: Georgina
authors.initials: G
authors.lastname: Díaz
authors.forename: Roberto
authors.initials: R
authors.lastname: Chavira
authors.forename: Cristina
authors.initials: C
authors.lastname: Fernandez-Mejia
mesh_terms: D000818:Animals; D001710:Biotin / Q000008:administration & dosage / Q000097:blood / Q000172:deficiency* / Q000494:pharmacology*; D001835:Body Weight / Q000187:drug effects; D004032:Diet; D004958:Estradiol / Q000097:blood; D030762:Estrous Cycle / Q000187:drug effects; D005260:Female; D008099:Liver / Q000033:anatomy & histology / Q000187:drug effects / Q000201:enzymology; D051379:Mice; D008807:Mice, Inbred BALB C; D009929:Organ Size / Q000187:drug effects; D010053:Ovary / Q000033:anatomy & histology / Q000187:drug effects; D011374:Progesterone / Q000097:blood; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011959:Receptors, Estradiol / Q000235:genetics; D011980:Receptors, Progesterone / Q000235:genetics; D012098:Reproduction / Q000187:drug effects* / Q000502:physiology*; D014599:Uterus / Q000187:drug effects / Q000378:metabolism
chemical_list: D012333:RNA, Messenger; D011959:Receptors, Estradiol; D011980:Receptors, Progesterone; D011374:Progesterone; D004958:Estradiol; D001710:Biotin