Collection and culture of alveolar bone marrow multipotent mesenchymal stromal cells from older individuals.
In this work, we examined the culture condition of alveolar bone marrow multipotent mesenchymal stromal cells (ABMMSCs), aiming to apply regenerative therapy to older periodontitis patients. To better understand the character of cultured cells from alveolar bone marrow, the expression profiles of well-known genes and their responses to the induction of osteogenic, chondrogenic, or adipogenic differentiation were examined. Using alpha MEM-based culture, ABMMSCs could be obtained from older individuals than in previous reports. Interestingly, ABMMSCs expressing Klf4 were able to differentiate into osteoblasts. The prediction of differentiation potential by Klf4 could be a useful guide for further improvement of the culture conditions required to culture ABMMSCs derived from older individuals.
Han
Juan
J
Department of Pediatric Dentistry, Nihon University School of Dentistry at Matsudo, Matsudo, Chiba, Japan.
Okada
Hironori
H
Takai
Hideki
H
Nakayama
Youhei
Y
Maeda
Takahide
T
Ogata
Yorimasa
Y
D000539:Alveolar Process / Q000166:cytology*; D001854:Bone Marrow Cells / Q000166:cytology; D018929:Cell Culture Techniques / Q000379:methods; D002454:Cell Differentiation; D005260:Female; D006801:Humans; D000090062:Kruppel-Like Factor 4; D051741:Kruppel-Like Transcription Factors; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D008875:Middle Aged; D039902:Multipotent Stem Cells / Q000166:cytology; D010006:Osteoblasts / Q000166:cytology; D010518:Periodontitis / Q000628:therapy; D044968:Regenerative Medicine; D017154:Stromal Cells / Q000166:cytology*
C000715467:KLF4 protein, human; D000090062:Kruppel-Like Factor 4; D051741:Kruppel-Like Transcription Factors