title: Neospora caninum and bone marrow-derived dendritic cells: parasite survival, proliferation, and induction of cytokine expression.
abstract: Dendritic cells (DCs) represent the first line defence of the innate immune system following infection with pathogens. We exploratively addressed invasion and survival ability of Neospora caninum, a parasite causing abortion in cattle, in mouse bone marrow DCs (BMDCs), and respective cytokine expression patterns. Immature BMDCs were exposed to viable (untreated) and nonviable parasites that had been inactivated by different means. Invasion and/or internalization, as well as intracellular survival and proliferation of tachyzoites were determined by NcGRA2-RT-PCR and transmission electron microscopy (TEM). Cytokine expression was evaluated by reverse transcription (RT)-PCR and cytokine ELISA. Transmission electron microscopy of DCs stimulated with untreated viable parasites revealed that N. caninum was able to invade and proliferate within BMDCs. This was confirmed by NcGRA2-RT-PCR. On the other hand, no viable parasite organisms were revealed by TEM when exposing BMDCs to inactivated parasites (nonviability demonstrated by NcGRA2-RT-PCR). Cytokine expression analysis (as assessed by both RT-PCR and ELISA) demonstrated that both viable and nonviable parasites stimulated mBMDCs to express IL-12p40, IL-10 and TNF-alpha, whereas IL-4 RNA expression was not detected. Thus, exposure of mBMDCs to both viable and nonviable parasites results in the expression of cytokines that are relevant for a mixed Th1/Th2 immune response.
authors.affiliation: Institute of Parasitology, University of Berne, Bern, Switzerland.
authors.forename: M
authors.initials: M
authors.lastname: Strohbusch
authors.forename: N
authors.initials: N
authors.lastname: Müller
authors.forename: A
authors.initials: A
authors.lastname: Hemphill
authors.forename: M
authors.initials: M
authors.lastname: Margos
authors.forename: D
authors.initials: D
authors.lastname: Grandgirard
authors.forename: S
authors.initials: S
authors.lastname: Leib
authors.forename: G
authors.initials: G
authors.lastname: Greif
authors.forename: B
authors.initials: B
authors.lastname: Gottstein
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D016207:Cytokines / Q000096:biosynthesis*; D003600:Cytosol / Q000469:parasitology; D003713:Dendritic Cells / Q000276:immunology* / Q000469:parasitology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D051379:Mice; D008810:Mice, Inbred C57BL; D050296:Microbial Viability; D046529:Microscopy, Electron, Transmission / Q000379:methods; D018688:Neospora / Q000254:growth & development* / Q000276:immunology*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods
chemical_list: D016207:Cytokines