Cold-adapted esterases and lipases: from fundamentals to application.
Micro-organisms that thrive at low temperatures produce cold-adapted enzymes which display high catalytic efficiency, generally associated with low thermal stability. In the recent past, researchers and industries have focused the attention on cold-adapted enzymes, whose peculiar properties make them particularly interesting either for investigating stability/flexibility relationships, or for their potential application in industrial processes. Among these enzymes, lipases and esterases, have potential utilisations in a broad range of biotechnological applications. In fact, these biocatalysts represent the most widely used enzymes in biotechnology and organic chemistry. Modern methods of genetic engineering combined with growing knowledge of structure and function allow further adaptation to industrial needs and exploration of novel applications. Hence, in this review we attempt to offer an overview on some psychrophilic esterases and lipases; major details will be presented for ORF PSHAa0051 from Pseudoalteromonas haloplanktis TAC125, recently investigated by our team. In addition, potential biotechnological applications will be discussed.
Tutino
M Luisa
ML
Department of Organic Chemistry and Biochemistry, University of Naples Federico II, Complesso Universitario Monte Sant'Angelo, Via Cinthia 4, I-80126 Naples, Italy.
di Prisco
Guido
G
Marino
Gennaro
G
de Pascale
Donatella
D
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D003080:Cold Temperature; D004950:Esterases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D008049:Lipase / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D008956:Models, Chemical; D008969:Molecular Sequence Data; D017434:Protein Structure, Tertiary; D031161:Pseudoalteromonas / Q000201:enzymology / Q000235:genetics / Q000254:growth & development; D013379:Substrate Specificity
D001426:Bacterial Proteins; D004950:Esterases; D008049:Lipase