Rapid detection of matrix metalloproteinase-7 and carcinoembryonic antigen mRNA expression in intraoperative pleural lavage samples using the reverse transcriptase loop-mediated isothermal amplification method.
OBJECTIVE
To detect the RNA of the tumor origin in intraoperative pleural lavage accurately by examining using rapid nucleic acid amplification method because patients with lung cancer often have recurrence detected by a cytologic examination of cancer cells in intraoperative pleural lavage.


METHODS
We used reverse transcriptase loop-mediated isothermal amplification (RT-LAMP), which enables rapid gene amplification, and examined the expression of matrix metalloproteinase-7 (MMP-7) and carcinoembryonic antigen (CEA) mRNA in intraoperative pleural lavage samples obtained from 79 consecutive surgical patients with lung adenocarcinoma.


RESULTS
MMP-7 mRNA was detected in cancer tissues from 76 (96%) of those cases, while it was also detected in the intraoperative pleural lavage samples of 23 (30%) of those 76 cases. In addition, CEA mRNA was detected in cancer tissues from 55 (70%) cases and in 8 (15%) of the lavage samples. MMP-7 mRNA-positive cases showed a significantly positive correlation with the p and n factors, and moderate to poor differentiation. Three cases shown to be positive in cytology examinations were also positive for both MMP-7 and CEA mRNA by RT-LAMP.


CONCLUSIONS
This detection method can be utilized during an operation and may provide useful information.
Ikeda
Satoshi
S
Department of Pathology, Tsuchiura Kyodo General Hospital, 11-7 Manabe-shinmachi, Tsuchiura 300-0053, Japan. isohama2587@proof.ocn.ne.jp
Inagaki
Masaharu
M
Takabe
Kazuhiko
K
Kumagai
Jiro
J
Suzuki
Keiko
K
D000230:Adenocarcinoma / Q000175:diagnosis / Q000235:genetics / Q000601:surgery; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000235:genetics / Q000378:metabolism; D002272:Carcinoembryonic Antigen / Q000235:genetics* / Q000378:metabolism; D003581:Cytodiagnosis / Q000379:methods / Q000639:trends; D005260:Female; D015870:Gene Expression; D006801:Humans; D007430:Intraoperative Care; D008175:Lung Neoplasms / Q000175:diagnosis / Q000235:genetics / Q000601:surgery; D008297:Male; D020783:Matrix Metalloproteinase 7 / Q000235:genetics* / Q000378:metabolism; D008875:Middle Aged; D035422:Pleural Cavity / Q000473:pathology*; D012333:RNA, Messenger / Q000378:metabolism; D012334:RNA, Neoplasm / Q000032:analysis*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*
D014408:Biomarkers, Tumor; D002272:Carcinoembryonic Antigen; D012333:RNA, Messenger; D012334:RNA, Neoplasm; C060932:MMP7 protein, human; D020783:Matrix Metalloproteinase 7