title: Influence of polyelectrolyte capillary coating conditions on protein analysis in CE.
abstract: CE of biomolecules is limited by analyte adsorption on the capillary wall. To prevent this, monolayer or successive multiple ionic-polymer layers (SMILs) of highly charged polyelectrolytes can be physically adsorbed on the inner capillary surface. Although these coatings have become commonly used in CE, no systematic investigation of their performance under different coating conditions has been carried out so far. In a previous study (Nehmé, R., Perrin, C., Cottet, H., Blanchin, M. D., Fabre, H., Electrophoresis 2008, 29, 3013-3023), we investigated the influence of different experimental parameters on coating stability, repeatability and peptide peak efficiency. Optimal coating conditions for monolayer and multilayer (SMILs) poly(diallyldimethylammonium) chloride/ poly(sodium 4-styrenesulfonate) coated capillaries were determined. In this study, the influence of polyelectrolyte concentration and ionic strength of the coating solutions, and the number of coating layers on coating stability and performance in limiting protein adsorption was carried out. EOF magnitude and repeatability were used to monitor coating stability. Coating ability to limit protein adsorption was investigated by monitoring variations of migration times, time-corrected peak areas and separation efficiency of test proteins. The separation performance of polyelectrolyte coatings were compared with those obtained with bare silica capillaries.
authors.affiliation: Institut des Biomolécules Max Mousseron, UMR, Université Montpellier 1, Université Montpellier 2, CNRS, Montpellier, France.
authors.forename: Reine
authors.initials: R
authors.lastname: Nehmé
authors.forename: Catherine
authors.initials: C
authors.lastname: Perrin
authors.forename: Hervé
authors.initials: H
authors.lastname: Cottet
authors.forename: Marie-Dominique
authors.initials: MD
authors.lastname: Blanchin
authors.forename: Huguette
authors.initials: H
authors.lastname: Fabre
mesh_terms: D000327:Adsorption; D004573:Electrolytes / Q000737:chemistry*; D053841:Electroosmosis; D019075:Electrophoresis, Capillary / Q000295:instrumentation / Q000379:methods*; D009994:Osmolar Concentration; D011095:Polyethylenes / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*; D011489:Protein Denaturation; D017510:Protein Folding; D011506:Proteins / Q000737:chemistry / Q000302:isolation & purification*; D000644:Quaternary Ammonium Compounds / Q000737:chemistry*; D015203:Reproducibility of Results; D013451:Sulfonic Acids / Q000737:chemistry
chemical_list: D004573:Electrolytes; D011095:Polyethylenes; D011108:Polymers; D011506:Proteins; D000644:Quaternary Ammonium Compounds; D013451:Sulfonic Acids; C041004:poly-N,N-dimethyl-N,N-diallylammonium chloride; C077114:styrenesulfonic acid polymer