[The relationship between CD4+ CD25+ regulatory T cell in the peripheral blood and poor or nonresponsiveness to hepatitis B vaccine].
OBJECTIVE
To investigate CD4+ CD25+ regulatory T cell frequencies in the peripheral blood of poor or non-responsiveness to Hepatitis B vaccine, and try to understand the relationship between CD4+ CD25+ regulatory T cell and poor or non-responsiveness to Hepatitis B vaccine.


METHODS
Flow cytometric analysis was employed for CD4+ CD25+ regulatory T cell frequencies in the peripheral blood of 25 cases of non-responsiveness, 30 cases of poor-responsiveness, and collected 20 cases of responsiveness as control.


RESULTS
CD4+ CD25+ regulatory T cell frequencies of responsiveness was (4.32 +/- 1.21)%, poor-responsiveness was (7.01 +/- 1.06)% and non-responsiveness was (12.75 +/- 2.01)%. It was found that non and poor-responsiveness showed a high percentage of CD4+ CD25+ regulatory T cell as compared with responsiveness (t = 8.426, t = 3.289, P<0.01).


CONCLUSIONS
The poor and non-responsiveness should be related with the increase of CD4+ CD25+ regulatory T cell and this might be considered as an important cause of poor and non-responsiveness.
Niu
Wei
W
Department of Immunology, Third Military Medical University, Chongqing 400038, China.
Yang
Zhao
Z
Shang
Xiao-yun
XY
Fu
Xiao-lan
XL
Tang
Yan
Y
Jiang
Man
M
Wang
Li
L
D000293:Adolescent; D000328:Adult; D001769:Blood / Q000276:immunology; D015704:CD4 Antigens / Q000276:immunology; D005260:Female; D005434:Flow Cytometry; D017325:Hepatitis B Vaccines / Q000276:immunology*; D006801:Humans; D053645:Interleukin-2 Receptor alpha Subunit / Q000276:immunology; D008297:Male; D008875:Middle Aged; D016176:T-Lymphocyte Subsets / Q000276:immunology*; D050378:T-Lymphocytes, Regulatory / Q000276:immunology* / Q000378:metabolism; D055815:Young Adult
D015704:CD4 Antigens; D017325:Hepatitis B Vaccines; D053645:Interleukin-2 Receptor alpha Subunit