title: Investigating the effect of temperature on transient partial unfolding by proteolysis.
abstract: Protein inactivation frequently occurs through partially unfolded states under native conditions, and temperature is an important parameter that affects the susceptibility of proteins to inactivation. While the effect of temperature on global unfolding is well documented, however, experimental characterizations of the temperature effect on partial unfolding are rare. Proteolysis offers a valuable chance to investigate the temperature effect on partial unfolding. By investigating proteolysis kinetics, the energetics of the partially unfolded state responsible for proteolysis (the cleavable state) can be studied. E. coli ribonuclease H (RNase H) has been shown to be cleaved by thermolysin at the amide bond between Thr92 and Ala93 through partial unfolding. Using this cleavage as a model system, we evaluated quantitatively the temperature effect on conformational equilibrium between the native state and a cleavable state. The analysis shows that decrease in temperature from 37 degrees C to 4 degrees C decreases the population in the cleavable state and reduces proteolytic susceptibility of the substrate protein. The conformational change leading to the cleavable state has a temperature-independent positive DeltaH degrees with negligible DeltaC(p) degrees . This thermodynamic characteristic of partial unfolding for proteolysis is quite distinct from that of global unfolding of RNase H that has a considerable DeltaC(p) degrees and a negative DeltaH degrees at low temperature. The distinct thermodynamic characteristics of partial unfolding from global unfolding mainly result from the difference in the changes of solvent-accessible surface area, which confirmed that the temperature effect on partial unfolding is strongly scale-dependent.
authors.affiliation: Department of Medicinal Chemistry and Molecular Pharmacology and Bindley Bioscience Center, 575 Stadium Mall Drive, Purdue University, West Lafayette, Indiana 47907, USA.
authors.forename: Kiwon
authors.initials: K
authors.lastname: Youn
authors.forename: Chiwook
authors.initials: C
authors.lastname: Park
mesh_terms: D029968:Escherichia coli Proteins / Q000737:chemistry* / Q000378:metabolism; D006868:Hydrolysis; D007700:Kinetics; D008958:Models, Molecular; D011489:Protein Denaturation; D017510:Protein Folding; D016914:Ribonuclease H / Q000737:chemistry* / Q000378:metabolism; D013696:Temperature; D013816:Thermodynamics; D013820:Thermolysin / Q000378:metabolism
chemical_list: D029968:Escherichia coli Proteins; D016914:Ribonuclease H; D013820:Thermolysin