title: Zebrafish beta-adrenergic receptor mRNA expression and control of pigmentation.
abstract: Beta adrenergic receptors (beta-ARs) are members of the G-protein-coupled receptor superfamily and mediate various physiological processes in many species. The expression patterns and functions of beta-ARs in zebrafish are, however, largely unknown. We have identified zebrafish beta-AR orthologs, which we have designated as adrb1, adrb2a, adrb2b, adrb3a and adrb3b. adrb1 was found to be expressed in the heart and brain. Expression of adrb2a predominated in the brain and skin, whereas adrb2b was found to be highly expressed in muscle, pancreas and liver. Both adrb3a and adrb3b were exclusively expressed in blood. Knock-down of these beta-ARs by morpholino oligonucleotides revealed a functional importance of adrb2a in pigmentation. Expression of atp5a1 and atp5b, genes that encode subunits of F1F0-ATPase, which is known to be involved in pigmentation, was significantly increased by knock-down of adrb2a. Our data suggest that adrb2a may regulate pigmentation, partly by modulating F1F0-ATPase.
authors.affiliation: Department of Molecular and Cellular Pharmacology, Pharmacogenomics and Pharmacoinformatics, Mie University Graduate School of Medicine, Tsu, Mie, Japan.
authors.forename: Zhipeng
authors.initials: Z
authors.lastname: Wang
authors.forename: Yuhei
authors.initials: Y
authors.lastname: Nishimura
authors.forename: Yasuhito
authors.initials: Y
authors.lastname: Shimada
authors.forename: Noriko
authors.initials: N
authors.lastname: Umemoto
authors.forename: Minoru
authors.initials: M
authors.lastname: Hirano
authors.forename: Liqing
authors.initials: L
authors.lastname: Zang
authors.forename: Takehiko
authors.initials: T
authors.lastname: Oka
authors.forename: Chikara
authors.initials: C
authors.lastname: Sakamoto
authors.forename: Junya
authors.initials: J
authors.lastname: Kuroyanagi
authors.forename: Toshio
authors.initials: T
authors.lastname: Tanaka
mesh_terms: D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D020319:Oligodeoxyribonucleotides, Antisense / Q000235:genetics; D010641:Phenotype; D010802:Phylogeny; D010858:Pigmentation / Q000235:genetics* / Q000502:physiology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011943:Receptors, Adrenergic, beta / Q000235:genetics* / Q000502:physiology; D013045:Species Specificity; D014018:Tissue Distribution; D015027:Zebrafish / Q000196:embryology / Q000235:genetics* / Q000502:physiology*; D029961:Zebrafish Proteins / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000502:physiology
chemical_list: D018076:DNA, Complementary; D020319:Oligodeoxyribonucleotides, Antisense; D012333:RNA, Messenger; D011943:Receptors, Adrenergic, beta; D029961:Zebrafish Proteins