title: Importance of junctional adhesion molecule-C for neointimal hyperplasia and monocyte recruitment in atherosclerosis-prone mice-brief report.
abstract: OBJECTIVE
  Although junctional adhesion molecule (JAM)-C has been implicated in the control of inflammatory leukocyte recruitment, its role in neointima formation after arterial injury has not been elucidated.
  
  
  RESULTS
  In apolipoprotein E-deficient (Apoe(-/-)) mice fed an atherogenic diet, antibody blockade of JAM-C significantly reduced neointimal hyperplasia after wire injury of carotid arteries without altering medial area and decreased neointimal macrophage but not smooth muscle cell (SMC) content. An increased expression of JAM-C was detected in colocalization with luminal SMCs 1 day after injury and neointimal SMCs after 3 weeks. Blocking JAM-C inhibited monocytic cell arrest and leukocyte adhesion to carotid arteries perfused ex vivo and in vivo. Furthermore, monocyte adhesion to activated coronary artery SMCs under flow conditions in vitro was diminished by blocking JAM-C.
  
  
  CONCLUSIONS
  Our data provide the first evidence for a crucial role of JAM-C in accelerated lesion formation and leukocyte recruitment in atherosclerosis-prone mice.
authors.affiliation: Institut für Molekulare Herz-Kreislaufforschung, Universitätsklinikum der RWTH Aachen, Pauwelsstrasse 30, 52074 Aachen, Germany.
authors.forename: Erdenechimeg
authors.initials: E
authors.lastname: Shagdarsuren
authors.forename: Yassin
authors.initials: Y
authors.lastname: Djalali-Talab
authors.forename: Michel
authors.initials: M
authors.lastname: Aurrand-Lions
authors.forename: Kiril
authors.initials: K
authors.lastname: Bidzhekov
authors.forename: Elisa A
authors.initials: EA
authors.lastname: Liehn
authors.forename: Beat A
authors.initials: BA
authors.lastname: Imhof
authors.forename: Christian
authors.initials: C
authors.lastname: Weber
authors.forename: Alma
authors.initials: A
authors.lastname: Zernecke
mesh_terms: D000818:Animals; D050197:Atherosclerosis / Q000378:metabolism / Q000473:pathology*; D002339:Carotid Arteries / Q000378:metabolism / Q000473:pathology*; D002448:Cell Adhesion; D015815:Cell Adhesion Molecules / Q000378:metabolism*; D004195:Disease Models, Animal; D005434:Flow Cytometry; D006965:Hyperplasia; D007136:Immunoglobulins / Q000378:metabolism*; D051379:Mice; D009000:Monocytes / Q000473:pathology*; D017539:Tunica Intima / Q000378:metabolism / Q000473:pathology*
chemical_list: D015815:Cell Adhesion Molecules; D007136:Immunoglobulins; C486723:Jam3 protein, mouse