title: Characterization of in vivo effects of platelet-rich plasma and biodegradable gelatin hydrogel microspheres on degenerated intervertebral discs.
abstract: We have previously shown that administration of platelet-rich plasma-impregnated gelatin hydrogel microspheres (PRP-GHMs) into a degenerated intervertebral disc (IVD) markedly suppresses progression of IVD degeneration. In the current study, we characterized the in vivo effects of PRP-GHM treatment in a degenerated IVD model in rabbit. On magnetic resonance images, the IVD height was significantly greater after treatment with PRP-GHMs compared with phosphate-buffered saline-impregnated GHMs, PRP without GHMs, and needle puncture only. Water content was also preserved in PRP-GHM-treated IVDs. Consistent with this observation, the mRNA expression of proteoglycan core protein and type II collagen was significantly higher after PRP-GHM treatment compared with other treatment groups. No proliferating cells were found in the nucleus pulposus and inner annulus fibrosus in any groups, but the number of apoptotic cells in the nucleus pulposus after PRP-GHM treatment was significantly lower than that after other treatments. These results provide an improved understanding of the therapeutic effects of PRP-GHM treatment of degenerated IVDs.
authors.affiliation: Department of Orthopaedics, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, Kyoto, Japan. sawamura0513@cup.ocn.ne.jp
authors.forename: Kazuhide
authors.initials: K
authors.lastname: Sawamura
authors.forename: Takumi
authors.initials: T
authors.lastname: Ikeda
authors.forename: Masateru
authors.initials: M
authors.lastname: Nagae
authors.forename: Shin-ichi
authors.initials: S
authors.lastname: Okamoto
authors.forename: Yasuo
authors.initials: Y
authors.lastname: Mikami
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Hase
authors.forename: Kazuya
authors.initials: K
authors.lastname: Ikoma
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Yamada
authors.forename: Hirotaka
authors.initials: H
authors.lastname: Sakamoto
authors.forename: Ken-ichi
authors.initials: K
authors.lastname: Matsuda
authors.forename: Yasuhiko
authors.initials: Y
authors.lastname: Tabata
authors.forename: Mitsuhiro
authors.initials: M
authors.lastname: Kawata
authors.forename: Toshikazu
authors.initials: T
authors.lastname: Kubo
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D001672:Biocompatible Materials / Q000494:pharmacology*; D002417:Cattle; D049109:Cell Proliferation / Q000187:drug effects; D024043:Collagen Type II / Q000235:genetics / Q000378:metabolism; D003692:Delayed-Action Preparations / Q000494:pharmacology; D005780:Gelatin / Q000494:pharmacology*; D020869:Gene Expression Profiling; D006801:Humans; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000494:pharmacology*; D020287:In Situ Nick-End Labeling; D007403:Intervertebral Disc / Q000187:drug effects / Q000473:pathology*; D055959:Intervertebral Disc Degeneration / Q000473:pathology / Q000628:therapy*; D008279:Magnetic Resonance Imaging; D008863:Microspheres; D053657:Platelet-Rich Plasma / Q000378:metabolism*; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism; D011509:Proteoglycans / Q000235:genetics / Q000378:metabolism; D011817:Rabbits; D016212:Transforming Growth Factor beta / Q000494:pharmacology
chemical_list: D001672:Biocompatible Materials; D024043:Collagen Type II; D003692:Delayed-Action Preparations; D018809:Proliferating Cell Nuclear Antigen; D011509:Proteoglycans; D016212:Transforming Growth Factor beta; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D005780:Gelatin