title: Construction and immunogenicity of a DNA vaccine containing clumping factor A of Staphylococcus aureus and bovine IL18.
abstract: Selection of potent cytokine adjuvants is important for the development of Staphylococcus aureus DNA vaccines. Several potential cytokines have been proven to induce enhanced immune responses in animal models and clinical tests. There is still no reported use of IL18 as an adjuvant to design DNA vaccines against S. aureus. In this study, we cloned the main fibronectin binding protein gene (a fragment from clumping factor A, ClfA(221-550)) of S. aureus and bovine interleukin 18 (bIL18). Then recombinant plasmids were constructed based on the eukaryotic expression vector pVAX1 with or without bIL18. Indirect immunofluorescence assays in transfected HeLa cells indicated that the recombinant DNAs (rDNAs) could be expressed correctly and had antigenicity. BALB/c mice were used as experimental models to examine the immunogenicity of rDNAs in vivo. The ClfA(221-550) rDNA provoked antibody production. The bIL18 rDNA induced production of the Th1 type cytokines IL2 and IFNgamma, and ClfA(221-550) and bIL18 synergistically stimulated T-lymphocyte proliferation. The data demonstrated that bIL18 is a potent adjuvant that could be used to enhance cellular immunity.
authors.affiliation: College of Animal Science and Veterinary Medicine, Jilin University, 5333 Xi'an Road, Changchun 130062, Jilin, China.
authors.forename: Rong-Lan
authors.initials: RL
authors.lastname: Yin
authors.forename: Chang
authors.initials: C
authors.lastname: Li
authors.forename: Zheng-Tao
authors.initials: ZT
authors.lastname: Yang
authors.forename: Yan-Jing
authors.initials: YJ
authors.lastname: Zhang
authors.forename: Wen-Lin
authors.initials: WL
authors.lastname: Bai
authors.forename: Xiao
authors.initials: X
authors.lastname: Li
authors.forename: Rong-Huan
authors.initials: RH
authors.lastname: Yin
authors.forename: Hui
authors.initials: H
authors.lastname: Liu
authors.forename: Shan
authors.initials: S
authors.lastname: Liu
authors.forename: Qi
authors.initials: Q
authors.lastname: Yang
authors.forename: Yong-Guo
authors.initials: YG
authors.lastname: Cao
authors.forename: Nai-Sheng
authors.initials: NS
authors.lastname: Zhang
mesh_terms: D000276:Adjuvants, Immunologic / Q000008:administration & dosage; D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis; D001428:Bacterial Vaccines / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification; D001483:Base Sequence; D002417:Cattle; D003030:Coagulase / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*; D016207:Cytokines / Q000097:blood; D017931:DNA Primers / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D005260:Female; D019084:Fluorescent Antibody Technique, Indirect; D006367:HeLa Cells; D006801:Humans; D020382:Interleukin-18 / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*; D008213:Lymphocyte Activation; D008414:Mastitis, Bovine / Q000276:immunology* / Q000382:microbiology / Q000517:prevention & control*; D051379:Mice; D008807:Mice, Inbred BALB C; D010957:Plasmids / Q000235:genetics; D013203:Staphylococcal Infections / Q000276:immunology / Q000382:microbiology / Q000517:prevention & control / Q000662:veterinary*; D013211:Staphylococcus aureus / Q000235:genetics / Q000276:immunology* / Q000472:pathogenicity; D018417:Th1 Cells / Q000276:immunology; D014162:Transfection; D019444:Vaccines, DNA / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification
chemical_list: D000276:Adjuvants, Immunologic; D000907:Antibodies, Bacterial; D001428:Bacterial Vaccines; C515944:ClfA protein, Staphylococcus aureus; D003030:Coagulase; D016207:Cytokines; D017931:DNA Primers; D004269:DNA, Bacterial; D020382:Interleukin-18; D019444:Vaccines, DNA