Design and synthesis of novel hybrid metal complex-DNA conjugates: key building blocks for multimetallic linear DNA nanoarrays.
We describe here a short and efficient synthetic route for incorporating terpyridine based metal complexes at the 3'-extremity of both single and bis-oligonucleotide (bis-ODN) stretches to form novel metal complex-ODN conjugates. All single stranded mono and bis-ODN tethered metal complexes and the respective duplex ODNs were characterized by circular dichroism spectroscopy and UV-Vis melting experiments. Duplexes formed by these hybrid metal complex-DNA conjugates showed around 4-5 degrees C stabilization with respect to the unmodified duplexes. Moreover hybridization of two complementary bis-ODN tethered metal complexes at different ratios in solution gave rise to a self-assembled nanometric linear network, which was characterized by non-denaturing gel electrophoresis and TEM studies. Thus, our simple synthetic strategy would contribute to develop multimetallic 2D-DNA nanoarrays where we can place different metal complexes at regular intervals along the ODN stretches.
Ghosh
Sumana
S
Département de Chimie Moléculaire UMR CNRS 5250, Université Joseph Fourier BP 53, 38041, Grenoble Cedex 9, France.
Pignot-Paintrand
Isabelle
I
Dumy
Pascal
P
Defrancq
Eric
E
D002942:Circular Dichroism; D004247:DNA / Q000737:chemistry*; D004591:Electrophoresis, Polyacrylamide Gel; D007501:Iron / Q000737:chemistry*; D008024:Ligands; D046529:Microscopy, Electron, Transmission; D015394:Molecular Structure; D036103:Nanotechnology; D020411:Oligonucleotide Array Sequence Analysis; D009841:Oligonucleotides / Q000737:chemistry; D009942:Organometallic Compounds / Q000138:chemical synthesis* / Q000737:chemistry; D010316:Particle Size; D012428:Ruthenium / Q000737:chemistry*; D013237:Stereoisomerism; D013499:Surface Properties; D044366:Transition Temperature
D008024:Ligands; D009841:Oligonucleotides; D009942:Organometallic Compounds; D012428:Ruthenium; D004247:DNA; D007501:Iron