Induced pluripotent stem cells at nanoscale.
Reprogramming of mouse and human somatic cells into induced pluripotent stem (iPS) cells has been made possible with the expression of the transcription factor quartet Oct4, Sox2, c-Myc, and Klf4. Here, we compared iPS cells derived from mouse embryonic fibroblasts with the 4 factors to embryonic stem cells by electron microscopy. Both cell types are almost indistinguishable at the ultrastructural level, providing further evidence for the similarity of these 2 pluripotent stem cell populations.
Zeuschner
Dagmar
D
Department of Cell and Developmental Biology, Max Planck Institute for Molecular Biomedicine , Münster, Germany.
Mildner
Karina
K
Zaehres
Holm
H
Schöler
Hans R
HR
D000818:Animals; D002478:Cells, Cultured; D065150:Cellular Reprogramming / Q000187:drug effects*; D053595:Embryonic Stem Cells / Q000166:cytology; D005347:Fibroblasts / Q000166:cytology; D057026:Induced Pluripotent Stem Cells / Q000166:cytology*; D000090062:Kruppel-Like Factor 4; D051741:Kruppel-Like Transcription Factors / Q000494:pharmacology; D051379:Mice; D008854:Microscopy, Electron; D050814:Octamer Transcription Factor-3 / Q000494:pharmacology; D016271:Proto-Oncogene Proteins c-myc / Q000494:pharmacology; D055748:SOXB1 Transcription Factors / Q000494:pharmacology; D014157:Transcription Factors / Q000494:pharmacology*
C000715467:KLF4 protein, human; C000715468:Klf4 protein, mouse; D000090062:Kruppel-Like Factor 4; D051741:Kruppel-Like Transcription Factors; C489428:Myc protein, mouse; D050814:Octamer Transcription Factor-3; C494174:Pou5f1 protein, mouse; D016271:Proto-Oncogene Proteins c-myc; D055748:SOXB1 Transcription Factors; C527783:Sox2 protein, mouse; D014157:Transcription Factors