Highly expressed recombinant human follicle-stimulating hormone from Chinese hamster ovary cells grown in serum-free medium and its effect on induction of folliculogenesis and ovulation.
OBJECTIVE
To develop efficient Chinese hamster ovary (CHO) cells that express recombinant human FSH (rhFSH) in serum-free conditions and to investigate the effect of this newly synthesized rhFSH on folliculogenesis and ovulation.


METHODS
Experimental study.


METHODS
Seoul National University, South Korea.


METHODS
Forty immature hypophysectomized rats and 40 androgen-sterilized mice.


METHODS
A stable single CHO cell that expresses rhFSH at a high level was obtained by introducing the human chorionic gonadotropin (hCG) alpha-subunit and FSH beta-subunit genes. After purification processing, we investigated the effect of this newly synthesized rhFSH on folliculogenesis in hypophysectomized rats and ovulation in androgen-sterilized mice.


METHODS
The ovary weight, uterine weight, number of follicles, and ovarian morphology were evaluated in immature hypophysectomized rats. The number of ovulated oocytes and ovarian morphology were examined in androgen-sterilized mice.


RESULTS
After purification processing, we analyzed the new rhFSH using matrix-associated laser desorption ionization-time of flight and found that this new rhFSH increased both ovarian weight and uterine weight in hypophysectomized rats and induced ovulation in androgen-sterilized mice.


CONCLUSIONS
This newly synthesized rhFSH might be safely used in anovulatory infertile woman as well as in ovulation induction protocols for subfertile women.
Kim
Dong-Jae
DJ
Department of Laboratory Animal Medicine, College of Veterinary Medicine and BK2 Program for Veterinary Science, Seoul National University, Seoul, South Korea.
Seok
Seung-Hyeok
SH
Baek
Min-Won
MW
Lee
Hui-Young
HY
Juhn
Jae-Hyeon
JH
Lee
Seungwon
S
Yun
Minae
M
Park
Jae-Hak
JH
D000818:Animals; D016466:CHO Cells; D006224:Cricetinae; D003412:Cricetulus; D016895:Culture Media, Serum-Free; D005260:Female; D043373:Follicle Stimulating Hormone, Human / Q000494:pharmacology*; D006801:Humans; D007016:Hypophysectomy; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D009929:Organ Size; D006080:Ovarian Follicle / Q000187:drug effects / Q000502:physiology*; D010053:Ovary / Q000187:drug effects; D010060:Ovulation / Q000187:drug effects*; D051381:Rats; D017207:Rats, Sprague-Dawley; D011994:Recombinant Proteins / Q000494:pharmacology*; D014162:Transfection; D014599:Uterus / Q000033:anatomy & histology / Q000187:drug effects
D016895:Culture Media, Serum-Free; D043373:Follicle Stimulating Hormone, Human; D011994:Recombinant Proteins