title: Resistance of the cell wall to degradation is a critical parameter for isolation of high quality RNA from natural isolates of Bacillus subtilis.
abstract: Natural isolates of Bacillus subtilis are known for their ability to produce a large panel of bioactive compounds. Unfortunately, their recalcitrance to conventional molecular techniques limits their transcript studies. In this work, difficulties to isolate RNA attributed to the cell wall were overcome, finally authorising powerful RT-PCR's.
authors.affiliation: Laboratoire ProBioGEM, UPRES-EA 1026, Polytech-Lille, IUT A, Université des Sciences et Technologies de Lille, Bd Paul Langevin, 59655, Villeneuve d'Ascq, France. jean-sebastien.guez@polytech-lille.fr
authors.forename: Jean-Sébastien
authors.initials: JS
authors.lastname: Guez
authors.forename: François
authors.initials: F
authors.lastname: Coutte
authors.forename: Anne-Sophie
authors.initials: AS
authors.lastname: Drucbert
authors.forename: Nour-Eddine
authors.initials: NE
authors.lastname: Chihib
authors.forename: Pierre-Marie
authors.initials: PM
authors.lastname: Danzé
authors.forename: Philippe
authors.initials: P
authors.lastname: Jacques
mesh_terms: D001412:Bacillus subtilis / Q000166:cytology* / Q000235:genetics; D001431:Bacteriological Techniques; D002473:Cell Wall / Q000378:metabolism*; D012329:RNA, Bacterial / Q000302:isolation & purification*; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D012329:RNA, Bacterial