title: MCE-electrochemical detection for following interactions of ssDNA and dsDNA with methylene blue.
abstract: The interaction between the organic dye, methylene blue and DNA has been studied by MCE with electrochemical detection. Interaction produces two different signals, one corresponding to free methylene blue and other, for the complex methylene blue-DNA. The hybridization between a ssDNA and a complementary sequence, specific to the severe acute respiratory syndrome virus, has been performed and studied in a thermoplastic olefin polymer of amorphous structure CE-microchip with an end-channel gold wire detector. Moreover, studies with a longer dsDNA, an expression vector involved in the transitory or stable expression in mammals cells, pFLAG-CMV4, has also been performed.
authors.affiliation: Departamento de Química Física y Analítica, Universidad de Oviedo, Asturias, Spain.
authors.forename: Mario
authors.initials: M
authors.lastname: Castaño-Alvarez
authors.forename: Ana
authors.initials: A
authors.lastname: Fernández-la-Villa
authors.forename: María Teresa
authors.initials: MT
authors.lastname: Fernández-Abedul
authors.forename: Agustín
authors.initials: A
authors.lastname: Costa-García
mesh_terms: D004247:DNA / Q000737:chemistry* / Q000378:metabolism; D004277:DNA, Single-Stranded / Q000737:chemistry* / Q000378:metabolism; D055664:Electrochemical Techniques / Q000379:methods; D019075:Electrophoresis, Capillary / Q000379:methods; D046209:Electrophoresis, Microchip / Q000379:methods*; D006046:Gold / Q000737:chemistry; D007364:Intercalating Agents / Q000737:chemistry* / Q000378:metabolism; D008751:Methylene Blue / Q000737:chemistry* / Q000378:metabolism; D009693:Nucleic Acid Hybridization
chemical_list: D004277:DNA, Single-Stranded; D007364:Intercalating Agents; D006046:Gold; D004247:DNA; D008751:Methylene Blue