title: Polymorphisms of human platelet alloantigens HPA-1 and HPA-2 associated with severe coronary artery disease.
abstract: OBJECTIVE
  Insofar as platelet membrane glycoprotein (GP) polymorphisms were identified as potential risk factors for coronary artery disease (CAD), we investigated the contribution of human platelet antigen (HPA)-1 (GPIIb/IIIa) and HPA-2 (GPIb/IX) alleles and haplotypes to CAD pathogenesis.
  
  
  METHODS
  Study subjects comprised 247 middle-age CAD patients and 316 age-, gender-, and race-matched controls; HPA genotyping was performed by polymerase chain reaction with sequence specific primers.
  
  
  RESULTS
  The frequencies of HPA-1b (P<.001) and HPA-2b (P<.001) alleles and HPA-1a/1b (P<.001), HPA-1b/1b (P<.001), and HPA-2a/2b (P<.001) genotypes were higher in patients than control subjects. Select HPA haplotypes comprising the HPA-1b/2a (Pc=2.2 × 10(-4)) and HPA-1b/2b (Pc=.001) haplotypes which were positively associated, and the HPA-1a/2a (Pc=3.2 × 10(-5)) which was negatively associated with CAD, confer a disease susceptibility and protective nature to these haplotypes. Multivariate analysis confirmed the positive association of HPA-1b/2a [adjusted odds ratio (aOR)=3.63; 95% CI=2.42-5.43] and HPA-1b/2b (aOR=2.92; 95% CI=1.43-5.94) haplotypes with CAD, after adjustment for a number of covariates.
  
  
  CONCLUSIONS
  Our results suggest that HPA-1/HPA-2 haplotypes may be considered to be a major risk factor for CAD in middle-aged Tunisians.
authors.affiliation: Research unit of Hematological and Autoimmune Diseases, Faculty of Pharmacy, University of Monastir, Monastir, Tunisia.
authors.forename: Nesrine
authors.initials: N
authors.lastname: Abboud
authors.forename: Haitham
authors.initials: H
authors.lastname: Amin
authors.forename: Lakdhar
authors.initials: L
authors.lastname: Ghazouani
authors.forename: Sonia
authors.initials: S
authors.lastname: Ben Haj Khalifa
authors.forename: Ali
authors.initials: A
authors.lastname: Ben Khalafallah
authors.forename: Fawzi
authors.initials: F
authors.lastname: Aded
authors.forename: Wassim Y
authors.initials: WY
authors.lastname: Almawi
authors.forename: Touhami
authors.initials: T
authors.lastname: Mahjoub
mesh_terms: D016824:Antigens, Human Platelet / Q000235:genetics*; D001792:Blood Platelets; D003324:Coronary Artery Disease / Q000235:genetics*; D005260:Female; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006239:Haplotypes; D006801:Humans; D039661:Integrin beta3; D007519:Isoantigens; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide; D012307:Risk Factors; D014416:Tunisia
chemical_list: C115212:2a alloantigen, human; C071221:2b alloantigen, human; D016824:Antigens, Human Platelet; C061248:ITGB3 protein, human; D039661:Integrin beta3; D007519:Isoantigens