A force-spectroscopy-based single-molecule metal-binding assay.
Quantifying metal-binding by force: A quantitative single-molecule force-spectroscopy-based assay is developed to measure the binding affinity of metal ions to proteins. The method uses the unfolding force of a protein as a direct probe to distinguish the apo and metal-ion-bound forms of that protein and quantify the partitioning between the two forms (see figure).
Cao
Yi
Y
Department of Chemistry, The University of British Columbia, 2036 Main Mall, Vancouver, BC, V6T 1Z1, Canada.
Er
Kai Shih
KS
Parhar
Rakesh
R
Li
Hongbin
H
D007700:Kinetics; D008670:Metals / Q000737:chemistry*; D018625:Microscopy, Atomic Force; D011485:Protein Binding; D011506:Proteins / Q000737:chemistry*; D011994:Recombinant Proteins / Q000737:chemistry; D013056:Spectrophotometry, Ultraviolet; D013816:Thermodynamics
D008670:Metals; D011506:Proteins; D011994:Recombinant Proteins