title: Ginsenoside Rg1 protects rat cardiomyocyte from hypoxia/reoxygenation oxidative injury via antioxidant and intracellular calcium homeostasis.
abstract: Ginsenoside Rg1 is a major active ingredient of Panax notoginseng radix which has demonstrated a number of pharmacological actions including a cardioprotective effect in vivo. This study investigated the protective effect and mechanism of ginsenoside Rg1 in cardiomyocytes hypoxia/reoxygenation (H/R) model. Pretreatment with ginsenoside Rg1 (60-120 microM) reduced lactate dehydrogenase release and increased cell viability in a dose-dependent manner. Fluorescence analysis demonstrated ginsenoside Rg1 reduced intracellular ROS and suppressed the intracellular [Ca(2+)] level. Cell lysate detected an increase of T-SOD, CAT, and GSH levels. The myocardial protection of ginsenoside Rg1 during H/R is partially due to its antioxidative effect and intracellular calcium homeostasis.
authors.affiliation: Beijing Institute of Radiation Medicine, Beijing 100850, PR China.
authors.forename: Dan
authors.initials: D
authors.lastname: Zhu
authors.forename: Lei
authors.initials: L
authors.lastname: Wu
authors.forename: Chun-Rong
authors.initials: CR
authors.lastname: Li
authors.forename: Xiao-Wei
authors.initials: XW
authors.lastname: Wang
authors.forename: Yong-Jie
authors.initials: YJ
authors.lastname: Ma
authors.forename: Zhi-Yin
authors.initials: ZY
authors.lastname: Zhong
authors.forename: Hai-Bao
authors.initials: HB
authors.lastname: Zhao
authors.forename: Jian
authors.initials: J
authors.lastname: Cui
authors.forename: Si-Fan
authors.initials: SF
authors.lastname: Xun
authors.forename: Xiu-Lan
authors.initials: XL
authors.lastname: Huang
authors.forename: Zhe
authors.initials: Z
authors.lastname: Zhou
authors.forename: Sheng-Qi
authors.initials: SQ
authors.lastname: Wang
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D002118:Calcium / Q000378:metabolism*; D015687:Cell Hypoxia; D002470:Cell Survival; D005260:Female; D036145:Ginsenosides / Q000494:pharmacology*; D006706:Homeostasis; D008297:Male; D032383:Myocytes, Cardiac / Q000187:drug effects* / Q000378:metabolism; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D010100:Oxygen / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D017382:Reactive Oxygen Species / Q000378:metabolism
chemical_list: D000975:Antioxidants; D036145:Ginsenosides; D017382:Reactive Oxygen Species; C035054:ginsenoside Rg1; D010100:Oxygen; D002118:Calcium