title: Inhibition of 11beta-hydroxysteroid dehydrogenase type 1 by plant extracts used as traditional antidiabetic medicines.
abstract: Elevated glucocorticoids are a key risk factor for metabolic diseases, and the glucocorticoid-activating enzyme 11beta-hydroxysteroid dehydrogenase 1 (11beta-HSD1) represents a promising therapeutic target. We measured the potential of six traditional antidiabetic medicinal plants extracts to inhibit 11beta-HSD1 activity and glucocorticoid receptor (GR) activation in transfected HEK-293 cells. Leave extracts of Eriobotrya japonica preferentially inhibited 11beta-HSD1 over 11beta-HSD2. Extracts of roasted but not native coffee beans preferentially inhibited 11beta-HSD1 over 11beta-HSD2, emphasizing the importance of sample preparation. Thus, natural compounds inhibiting 11beta-HSD1 may contribute to the antidiabetic effect of the investigated plant extracts.
authors.affiliation: Division of Molecular and Systems Toxicology, Department of Pharmaceutical Sciences, University of Basel, Klingelbergstrasse 50, CH-4056 Basel, Switzerland. christel.gumy@gmail.com
authors.forename: Christel
authors.initials: C
authors.lastname: Gumy
authors.forename: Carmen
authors.initials: C
authors.lastname: Thurnbichler
authors.forename: Evelyne M
authors.initials: EM
authors.lastname: Aubry
authors.forename: Zoltan
authors.initials: Z
authors.lastname: Balazs
authors.forename: Petra
authors.initials: P
authors.lastname: Pfisterer
authors.forename: Lisa
authors.initials: L
authors.lastname: Baumgartner
authors.forename: Hermann
authors.initials: H
authors.lastname: Stuppner
authors.forename: Alex
authors.initials: A
authors.lastname: Odermatt
authors.forename: Judith M
authors.initials: JM
authors.lastname: Rollinger
mesh_terms: D043205:11-beta-Hydroxysteroid Dehydrogenase Type 1 / Q000037:antagonists & inhibitors*; D002460:Cell Line; D040503:Coffea; D031983:Eriobotrya; D006801:Humans; D007004:Hypoglycemic Agents / Q000494:pharmacology*; D008519:Medicine, Traditional; D010936:Plant Extracts / Q000494:pharmacology*; D018515:Plant Leaves; D011965:Receptors, Glucocorticoid / Q000037:antagonists & inhibitors*; D012639:Seeds
chemical_list: D007004:Hypoglycemic Agents; D010936:Plant Extracts; D011965:Receptors, Glucocorticoid; D043205:11-beta-Hydroxysteroid Dehydrogenase Type 1