An autophosphorylation site of the protein kinase SOS2 is important for salt tolerance in Arabidopsis.
The protein kinase SOS2 (Salt Overly Sensitive 2) is essential for salt-stress signaling and tolerance in Arabidopsis. SOS2 is known to be activated by calcium-SOS3 and by phosphorylation at its activation loop. SOS2 is autophosphorylated in vitro, but the autophosphorylation site and its role in salt tolerance are not known. In this study, we identified an autophosphorylation site in SOS2 and analyzed its role in the responses of Arabidopsis to salt stress. Mass spectrometry analysis showed that Ser 228 of SOS2 is autophosphorylated. When this site was mutated to Ala, the autophosphorylation rate of SOS2 decreased. The substrate phosphorylation by the mutated SOS2 was also less than that by the wild-type SOS2. In contrast, changing Ser228 to Asp to mimic the autophosphorylation enhanced substrate phosphorylation by SOS2. Complementation tests in a sos2 mutant showed that the S228A but not the S228D mutation partially disrupted the function of SOS2 in salt tolerance. We also show that activation loop phosphorylation at Thr168 and autophosphorylation at Ser228 cannot substitute for each other, suggesting that both are required for salt tolerance. Our results indicate that Ser 228 of SOS2 is autophosphorylated and that this autophosphorylation is important for SOS2 function under salt stress.
Fujii
Hiroaki
H
Department of Botany and Plant Sciences, 2150 Batchelor Hall, University of California, Riverside, CA 92521, USA.
Zhu
Jian-Kang
JK
D000222:Adaptation, Physiological; D017360:Arabidopsis / Q000201:enzymology / Q000502:physiology*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D001483:Base Sequence; D015152:Blotting, Northern; D017931:DNA Primers; D013058:Mass Spectrometry; D016297:Mutagenesis, Site-Directed; D010766:Phosphorylation; D030821:Plants, Genetically Modified / Q000201:enzymology / Q000502:physiology; D017346:Protein Serine-Threonine Kinases / Q000235:genetics / Q000378:metabolism*; D012965:Sodium Chloride
D029681:Arabidopsis Proteins; D017931:DNA Primers; D012965:Sodium Chloride; C406738:SOS2 protein, Arabidopsis; D017346:Protein Serine-Threonine Kinases