title: Assessing interethnic admixture using an X-linked insertion-deletion multiplex.
abstract: In this study, a PCR multiplex was optimized, allowing the simultaneous analysis of 13 X-chromosome Insertion/deletion polymorphisms (INDELs). Genetic variation observed in Africans, Europeans, and Native Americans reveals high inter-population variability. The estimated proportions of X-chromosomes in an admixed population from the Brazilian Amazon region show a predominant Amerindian contribution (approximately 41%), followed by European (approximately 32%) and African (approximately 27%) contributions. The proportion of Amerindian contribution based on X-linked data is similar to the expected value based on mtDNA and Y-chromosome information. The accuracy for assessing interethnic admixture, and the high differentiation between African, European, and Native American populations, demonstrates the suitability of this INDEL set to measure ancestry proportions in three-hybrid populations, as it is the case of Latin American populations.
authors.affiliation: Laboratório de Genética Humana e Médica, Departamento de Patologia, Universidade Federal do Pará, CEP: 66075-970 Belém, Pará, Brazil.
authors.forename: Elzemar Martins
authors.initials: EM
authors.lastname: Ribeiro-Rodrigues
authors.forename: Ney Pereira Carneiro
authors.initials: NP
authors.lastname: dos Santos
authors.forename: Andrea Kely Campos Ribeiro
authors.initials: AK
authors.lastname: dos Santos
authors.forename: Rui
authors.initials: R
authors.lastname: Pereira
authors.forename: António
authors.initials: A
authors.lastname: Amorim
authors.forename: Leonor
authors.initials: L
authors.lastname: Gusmão
authors.forename: Marco Antonio
authors.initials: MA
authors.lastname: Zago
authors.forename: Sidney Emanuel Batista
authors.initials: SE
authors.lastname: dos Santos
mesh_terms: D044467:American Indian or Alaska Native / Q000235:genetics*; D041321:Chromosomes, Human, X / Q000235:genetics*; D041322:Chromosomes, Human, Y / Q000235:genetics; D006801:Humans; D054643:INDEL Mutation; D007197:Indians, Central American / Q000235:genetics; D007198:Indians, North American / Q000235:genetics; D007199:Indians, South American / Q000235:genetics; D016133:Polymerase Chain Reaction; D044469:Racial Groups / Q000235:genetics*