title: Microscopic particle discrimination using spatially-resolved Fourier-holographic light scattering angular spectroscopy.
abstract: We utilize Fourier-holographic light scattering angular spectroscopy to record the spatially resolved complex angular scattering spectra of samples over wide fields of view in a single or few image captures. Without resolving individual scatterers, we are able to generate spatially-resolved particle size maps for samples composed of spherical scatterers, by comparing generated spectra with Mie-theory predictions. We present a theoretical discussion of the fundamental principles of our technique and, in addition to the sphere samples, apply it experimentally to a biological sample which comprises red blood cells. Our method could possibly represent an efficient alternative to the time-consuming and laborious conventional procedure in light microscopy of image tiling and inspection, for the characterization of microscopic morphology over wide fields of view.
authors.forename: Timothy R
authors.initials: TR
authors.lastname: Hillman
authors.forename: Sergey A
authors.initials: SA
authors.lastname: Alexandrov
authors.forename: Thomas
authors.initials: T
authors.lastname: Gutzler
authors.forename: David D
authors.initials: DD
authors.lastname: Sampson