Polysaccharides of basidiomycetes. alkali-soluble polysaccharides from the mycelium of white rot fungus Ganoderma lucidum (Curt.: Fr.) P. Karst.
Two polysaccharides were isolated from submergedly cultured mycelium of the basidiomycete Ganoderma lucidum by extraction with alkali followed by fractionation with Fehling reagent. The polysaccharides were shown to be a linear (1-->3)-alpha-D-glucan and a highly branched xylomannan containing a backbone built up of (1-->3)-linked alpha-D-mannopyranose residues, the majority of which are substituted at O-4 by single beta-D-xylopyranose residues or by disaccharide fragments beta-D-Manp-(1-->3)-beta-D-Xylp-(1-->. Polysaccharide structures were elucidated by NMR spectroscopy in combination with methylation analysis and periodate oxidation. An interesting feature of the xylomannan is the simultaneous presence of alpha-D-mannopyranose and beta-D-mannopyranose residues, the first forming the backbone, and the second being the non-reducing terminal units of disaccharide side chains.
Evsenko
M S
MS
Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, 119991, Russia.
Shashkov
A S
AS
Avtonomova
A V
AV
Krasnopolskaya
L M
LM
Usov
A I
AI
D000468:Alkalies / Q000737:chemistry; D002240:Carbohydrate Sequence; D008969:Molecular Sequence Data; D025282:Mycelium / Q000737:chemistry / Q000378:metabolism; D011134:Polysaccharides / Q000737:chemistry* / Q000378:metabolism; D020940:Reishi / Q000737:chemistry* / Q000378:metabolism; D012995:Solubility
D000468:Alkalies; D011134:Polysaccharides