Glucose-induced alteration of accumulation of organotechnetium complexes accumulation in Pgp-negative tumor-bearing mice.
The biologic and microenvironmental factors determining (99m)Tc sestamibi (MIBI) and (99m)Tc tetrofosmin (TF) uptake in breast tumors are incompletely understood, especially in P-glycoprotein (Pgp)-negative tumors. We analyzed the influence of glucose administration on the uptake and retention of MIBI and TF in Pgp-negative tumor-bearing mice in vivo. Twenty (20) mice bearing Ehrlich ascites tumor cell (EATC) xenografts were divided into four groups: (1) MIBI, (2) MIBI+glucose, (3) TF, and (4) TF+glucose. Glucose was administered (5.0 g/kg body weight) intraperitoreally (i.p.) 1 hour before scintigraphy. There were significant differences between the E-UPR MIBI and MIBI+glucose groups (p = 0.009) and minor differences in L-UPR between these groups (p = 0.04). There was a significant inverse correlation between E-UPR of MIBI and glucose levels (r = 0.71, p = 0.02). Comparing the four groups, the highest E-UPR was obtained in the MIBI group (p = 0.006). Other parameters were not different in the MIBI and MIBI+glucose groups and in the TF and TF+glucose groups. Increased blood glucose level affected the MIBI uptake of tumor tissue, particularly for E-UPR. We suggest that these findings were due to basically decreased blood flow and secondarily decreased extracellular pH. However, glucose administration did not affect TF.
Ustun
Funda
F
Faculty of Medicine, Department of Nuclear Medicine, Canakkale Onsekiz Mart University, Canakkale, Turkey. fundaustun@comu.edu.tr
Durmus-Altun
Gulay
G
Cukur
Ziya
Z
Altaner
Semsi
S
Berkarda
Sakir
S
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1 / Q000172:deficiency* / Q000235:genetics; D000818:Animals; D001786:Blood Glucose / Q000187:drug effects / Q000378:metabolism; D002286:Carcinoma, Ehrlich Tumor / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D003627:Data Interpretation, Statistical; D005260:Female; D005947:Glucose / Q000494:pharmacology*; D008660:Metabolism / Q000187:drug effects; D051379:Mice; D008807:Mice, Inbred BALB C; D009369:Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D009943:Organophosphorus Compounds / Q000378:metabolism; D015609:Organotechnetium Compounds / Q000378:metabolism*; D019275:Radiopharmaceuticals / Q000378:metabolism; D017256:Technetium Tc 99m Sestamibi / Q000378:metabolism
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D001786:Blood Glucose; D009943:Organophosphorus Compounds; D015609:Organotechnetium Compounds; D019275:Radiopharmaceuticals; C078700:technetium tc-99m tetrofosmin; D017256:Technetium Tc 99m Sestamibi; D005947:Glucose