title: Cell fusion-induced activation of interferon-stimulated genes is not required for restriction of a herpes simplex virus VP16/ICP0 mutant in heterokarya formed between permissive and restrictive cells.
abstract: Herpes simplex virus VP16 and ICP0 mutants replicate efficiently in U2OS osteosarcoma cells but are restricted in other cell types. We previously showed that the restrictive phenotype is dominant in a transient cell fusion assay, suggesting that U2OS cells lack an antiviral mechanism present in other cells. Recent data indicate that unscheduled membrane fusion events can activate the expression of interferon-stimulated genes (ISGs) in fibroblasts, raising the possibility that our earlier results were due to a fusion-induced antiviral state. However, we show here that the permissive phenotype is also extinguished following fusion with Vero cells in the absence of ISG induction.
authors.affiliation: Alberta Institute for Viral Immunology, Department of Medical Microbiology & Immunology, University of Alberta, Edmonton, Alberta T6G 2S2, Canada.
authors.forename: Meaghan H
authors.initials: MH
authors.lastname: Hancock
authors.forename: Karen L
authors.initials: KL
authors.lastname: Mossman
authors.forename: James R
authors.initials: JR
authors.lastname: Smiley
mesh_terms: D000818:Animals; D002459:Cell Fusion; D045744:Cell Line, Tumor; D002522:Chlorocebus aethiops; D017405:Herpes Simplex Virus Protein Vmw65 / Q000172:deficiency*; D006801:Humans; D017874:Immediate-Early Proteins / Q000172:deficiency*; D007372:Interferons / Q000096:biosynthesis*; D018139:Simplexvirus / Q000276:immunology* / Q000502:physiology*; D044767:Ubiquitin-Protein Ligases / Q000172:deficiency*; D014709:Vero Cells; D014779:Virus Replication
chemical_list: D017405:Herpes Simplex Virus Protein Vmw65; D017874:Immediate-Early Proteins; D007372:Interferons; D044767:Ubiquitin-Protein Ligases; C051804:Vmw110 protein, Human herpesvirus 1