title: A force-spectroscopy-based single-molecule metal-binding assay.
abstract: Quantifying metal-binding by force: A quantitative single-molecule force-spectroscopy-based assay is developed to measure the binding affinity of metal ions to proteins. The method uses the unfolding force of a protein as a direct probe to distinguish the apo and metal-ion-bound forms of that protein and quantify the partitioning between the two forms (see figure).
authors.affiliation: Department of Chemistry, The University of British Columbia, 2036 Main Mall, Vancouver, BC, V6T 1Z1, Canada.
authors.forename: Yi
authors.initials: Y
authors.lastname: Cao
authors.forename: Kai Shih
authors.initials: KS
authors.lastname: Er
authors.forename: Rakesh
authors.initials: R
authors.lastname: Parhar
authors.forename: Hongbin
authors.initials: H
authors.lastname: Li
mesh_terms: D007700:Kinetics; D008670:Metals / Q000737:chemistry*; D018625:Microscopy, Atomic Force; D011485:Protein Binding; D011506:Proteins / Q000737:chemistry*; D011994:Recombinant Proteins / Q000737:chemistry; D013056:Spectrophotometry, Ultraviolet; D013816:Thermodynamics
chemical_list: D008670:Metals; D011506:Proteins; D011994:Recombinant Proteins