Preparation and characterization of zedoary turmeric oil-loaded insulin-modified sterically stabilized liposomes.
The poor selectivity of anticancer drugs often leads to their multiplicate dose-limiting toxicities in humans, which severely restricts their clinical application. In this study, a novel liposomal formulation of zedoary turmeric oil (ZTO) targeting the insulin receptor (IR) was prepared by covalently conjugating insulin to the terminal of the polyethylene glycol (PEG) chain of sterically stabilized liposomes. In vitro assays indicated that a higher uptake of insulin-modified sterically stabilized liposomes (ISSLs) was observed in SMMC-7721 hepatocarcinoma cells overexpressing insulin receptors. IC(50) values of ISSLs, NTLs (nontargeted liposomes), and ZTO injection (free ZTO) against SMMC-7721, determined by MTT assays, were 157.2, 256.7, and 43.3 microg x ml(-1), respectively. Plasma-clearance profiles of ZTO in the liposomal formulations were then compared with that of ZTO injection. The liposomal formulations showed much longer terminal half-lives (11.24 and 14.73 hours for ISSLs and NTLs, respectively) than that of ZTO injection (1.45 hours). All results above indicated the ISSLs were potentially useful for the treatment of IR (+) tumors and are worthy of further investigation.
Yang
Zhiwen
Z
Department of Pharmaceutical Sciences, Gannan Medical College, Ganzhou, China. menghua001@sina.com
Fu
Dahua
D
Zhu
Liang
L
Yang
Muhua
M
Cheng
Qilai
Q
D030024:Curcuma / Q000737:chemistry*; D004304:Dosage Forms; D004317:Doxorubicin / Q000031:analogs & derivatives; D006801:Humans; D007328:Insulin; D008081:Liposomes; D009369:Neoplasms / Q000188:drug therapy; D009821:Oils; D010936:Plant Extracts; D011092:Polyethylene Glycols
D004304:Dosage Forms; D007328:Insulin; D008081:Liposomes; D009821:Oils; D010936:Plant Extracts; C506643:liposomal doxorubicin; D011092:Polyethylene Glycols; D004317:Doxorubicin; C012596:turmeric extract