De novo synthesis of human dermis in vitro in the absence of a three-dimensional scaffold.
Neonatal human dermal fibroblasts cultured in vitro synthesize an organized and physically substantial three-dimensional extracellular matrix, without the addition of exogenous matrix components or synthetic scaffolds. De novo matrix synthesis proceeds in an orderly manner over a 21-d culture period and beyond. Analysis of the fibroblast phenotype, i.e., matrix synthesis by the fibroblasts, suggests that both serum and serum-free conditions are conducive to the production of a human tissue-engineered "dermal equivalent". We report that given the appropriate permissive environment, the fibroblasts establish and grow a tissue in vitro, which bears striking biochemical and physical resemblance to normal human dermis.
Pouyani
Tara
T
Research and Development, Organogenesis Inc, Canton, MA 02021, USA. t.pouyani@neu.edu
Ronfard
Vincent
V
Scott
Paul G
PG
Dodd
Carole M
CM
Ahmed
Aftab
A
Gallo
Richard L
RL
Parenteau
Nancy L
NL
D003094:Collagen / Q000378:metabolism* / Q000648:ultrastructure; D020405:Dermis / Q000502:physiology* / Q000648:ultrastructure; D004187:Disaccharides / Q000032:analysis; D005347:Fibroblasts / Q000502:physiology / Q000648:ultrastructure; D006801:Humans; D006820:Hyaluronic Acid / Q000096:biosynthesis; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D019063:Tenascin / Q000096:biosynthesis; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds
D004187:Disaccharides; D019063:Tenascin; D006820:Hyaluronic Acid; D003094:Collagen