title: Optimized nutrient medium for galanthamine production in Leucojum aestivum L. in vitro shoot system.
abstract: The common effect of NH4+, NO3-, KH2PO4 and sucrose on the biosynthesis of galanthamine by a Leucojum aestivum shoot culture was studied. Polynominal regression models were elaborated for the description of the galanthamine biosynthesis as a consequence of variation of the investigated variables (NH4+ between 0.20 and 0.54 g/L; NO3- between 1.44 and 3.44 g/L; KH2PO4 between 0.10 and 0.24 g/L, and sucrose between 30.00 and 60.00 g/L). Optimization procedures allowed us to establish the optimal concentrations of the investigated variables and to propose the modified MS nutrient medium, with 4.50 g/L KNO3, 0.89 g/L NH4NO3, 1.25 g/L (NH4)2SO4, 0.10 g/L KH2PO4 and 60 g/L sucrose, for the galanthamine production by a Leucojum aestivum shoot culture. The proposed modified MS medium provided considerable increase of both the production yield and the relative content of the target alkaloid in the alkaloid mixture.
authors.affiliation: Department of Microbial Biosynthesis and Biotechnologies, Laboratory in Plovdiv, The Stephan Angeloff Institute of Microbiology, Bulgarian Academy of Sciences, 26 Maritza Blvd., 4002 Plovdiv, Bulgaria.
authors.forename: Vasil
authors.initials: V
authors.lastname: Georgiev
authors.forename: Strahil
authors.initials: S
authors.lastname: Berkov
authors.forename: Milen
authors.initials: M
authors.lastname: Georgiev
authors.forename: Monique
authors.initials: M
authors.lastname: Burrus
authors.forename: Carles
authors.initials: C
authors.lastname: Codina
authors.forename: Jaume
authors.initials: J
authors.lastname: Bastida
authors.forename: Mladenka
authors.initials: M
authors.lastname: Ilieva
authors.forename: Atanas
authors.initials: A
authors.lastname: Pavlov
mesh_terms: D000470:Alkaloids / Q000096:biosynthesis / Q000737:chemistry; D018533:Biomass; D003470:Culture Media; D005702:Galantamine / Q000096:biosynthesis* / Q000737:chemistry / Q000302:isolation & purification; D008401:Gas Chromatography-Mass Spectrometry; D007700:Kinetics; D019668:Liliaceae / Q000378:metabolism*; D018520:Plant Shoots / Q000378:metabolism*
chemical_list: D000470:Alkaloids; D003470:Culture Media; D005702:Galantamine