title: Identification of plasmid-mediated quinolone resistance qnr-like genes in Romanian clinical isolates of Escherichia coli and Klebsiella pneumoniae.
abstract: A collection of putative ESBL-producing Escherichia coli (119 isolates) and Klebsiella pneumoniae (122 isolates) originating from extraintestinal human specimens was screened for qnrA, qnrB, and qnrS-like genes by PCR. Seven K. pneumoniae isolates, which were resistant to ciprofloxacin, were detected as carrying qnrA1-like genes, while one K. pneumoniae and one E. coli isolate were positive for qnrS-like determinant. The latter isolates were susceptible to ciprofloxacin. This is the first study identifying qnr-like genes in our area. Further studies are needed to document the contribution of the plasmid mediated quinolone resistance to the increase in bacterial resistance to fluoroquinolones in Romania.
authors.affiliation: Molecular Microbiology Laboratory, National Institute of Research-Development for Microbiology and Immunology Cantacuzino.
authors.forename: Codruţa-Romaniţa
authors.initials: CR
authors.lastname: Usein
authors.forename: Andi-Marian
authors.initials: AM
authors.lastname: Palade
authors.forename: Dorina
authors.initials: D
authors.lastname: Tatu-Chiţoiu
authors.forename: Simona
authors.initials: S
authors.lastname: Ciontea
authors.forename: Stefania
authors.initials: S
authors.lastname: Ceciu
authors.forename: Maria
authors.initials: M
authors.lastname: Nica
authors.forename: Maria
authors.initials: M
authors.lastname: Damian
mesh_terms: D000890:Anti-Infective Agents / Q000494:pharmacology; D001426:Bacterial Proteins / Q000032:analysis / Q000235:genetics*; D002939:Ciprofloxacin / Q000494:pharmacology; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics; D024901:Drug Resistance, Multiple, Bacterial; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification; D004927:Escherichia coli Infections / Q000188:drug therapy / Q000235:genetics / Q000382:microbiology*; D029968:Escherichia coli Proteins / Q000032:analysis / Q000235:genetics*; D005798:Genes, Bacterial; D006801:Humans; D007710:Klebsiella Infections / Q000188:drug therapy / Q000235:genetics / Q000382:microbiology*; D007711:Klebsiella pneumoniae / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification; D010957:Plasmids / Q000032:analysis / Q000235:genetics*; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D012383:Romania
chemical_list: D000890:Anti-Infective Agents; D001426:Bacterial Proteins; D004269:DNA, Bacterial; D029968:Escherichia coli Proteins; C485384:Qnr protein, E coli; C485385:Qnr protein, Klebsiella pneumoniae; D002939:Ciprofloxacin