Favorable effects of the detergent and enzyme extraction method for preparing decellularized bovine pericardium scaffold for tissue engineered heart valves.
Bovine pericardium has been extensively applied as the biomaterial for artificial heart valves and may potentially be used as a scaffold for tissue-engineered heart valves after decellularization. Although various methods of decellularization are currently available, it is unknown which method is the most ideal one for the decellularization for bovine pericardium. We compared three decellularization methods, namely, the detergent and enzyme extraction (DEE), the trypsin (TS), and the Triton X-100 and sodium-deoxycholate (TSD) method, to examine their efficacy on cell removal and their preservation of the mechanical function and the tissue matrix structure. Results indicated that decellularization was achieved by all the three methods as confirmed by hematoxylin-eosin staining, scanning electron microscopy, as well as quantitative DNA measurement. However, TS and TSD methods resulted in severe structural destruction of the bovine pericardium as shown by von Gieson staining and Gomori staining. Furthermore, both TS and TSD methods changed the mechanical property of the bovine pericardium, as evidenced by a lower elastic modulus, maximal-stress, maximal-disfiguration, maximal-load, and maximal-strain. In conclusion, the DEE method achieved both a complete decellularization and preservation of the mechanical function and tissue structure of the bovine pericardium. Thus, this method is superior to either the TS or the TSD method for preparing decellularized bovine pericardium scaffold for constructing tissue-engineered heart valves.
Yang
Min
M
Department of Cardiothoracic Surgery, Renji Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, China.
Chen
Chang-Zhi
CZ
Wang
Xue-Ning
XN
Zhu
Ya-Bin
YB
Gu
Y John
YJ
D000818:Animals; D002417:Cattle; D003840:Deoxycholic Acid / Q000737:chemistry; D003902:Detergents / Q000737:chemistry*; D006350:Heart Valve Prosthesis; D019918:Heart Valve Prosthesis Implantation; D006351:Heart Valves / Q000166:cytology; D008422:Materials Testing; D017830:Octoxynol / Q000737:chemistry; D010496:Pericardium / Q000737:chemistry / Q000166:cytology / Q000378:metabolism; D011897:Random Allocation; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds; D014357:Trypsin / Q000378:metabolism
D003902:Detergents; D003840:Deoxycholic Acid; D017830:Octoxynol; D014357:Trypsin