Cyclic stretch upregulates SDF-1alpha/CXCR4 axis in human saphenous vein smooth muscle cells.
Cyclic stretch (CS) mediates different cellular functions in vascular smooth muscle cells and involves in neointimal hyperplasia and subsequent atherosclerosis of vein grafts. Here, we investigated whether CS can modulate stromal cell-derived factor-1alpha (SDF-1alpha)/CXCR4 axis in human saphenous vein smooth muscle cells. We found CS induced the upregulation of SDF-1alpha and CXCR4 in human saphenous vein smooth muscle cells in vitro, which was dependent on PI3K/Akt/mTOR pathway. Furthermore, CS augmented human saphenous vein smooth muscle migration and focal adhesion kinase (FAK) activation by PI3K/Akt/mTOR pathway. Interestingly, the upregulation of SDF-1alpha/CXCR4 axis was instrumental in CS-induced saphenous vein smooth muscle cell migration and FAK activation, as showed by AMD3100, an inhibitor of SDF-1alpha/CXCR4 axis, partially but significantly blocked the CS-induced cellular effects. Thus, those data suggested SDF-1alpha/CXCR4 axis involves in CS-mediated cellular functions in human saphenous vein smooth muscle cells.
Li
Fei
F
Department of Cardiology, Xijing Hospital, Fourth Military Medical University, Xi'an, China.
Guo
Wen-yi
WY
Li
Wei-jie
WJ
Zhang
Dian-xin
DX
Lv
An-lin
AL
Luan
Rong-hua
RH
Liu
Bing
B
Wang
Hai-chang
HC
D002465:Cell Movement; D054377:Chemokine CXCL12 / Q000096:biosynthesis* / Q000235:genetics; D006801:Humans; D040542:Mechanotransduction, Cellular; D009131:Muscle, Smooth, Vascular / Q000378:metabolism* / Q000502:physiology; D032389:Myocytes, Smooth Muscle / Q000378:metabolism* / Q000502:physiology; D019718:Receptors, CXCR4 / Q000096:biosynthesis* / Q000235:genetics; D012501:Saphenous Vein / Q000166:cytology / Q000378:metabolism* / Q000502:physiology; D013718:Tensile Strength; D015854:Up-Regulation
D054377:Chemokine CXCL12; D019718:Receptors, CXCR4