title: Association of Filaggrin loss-of-function-mutations with atopic dermatitis and asthma in the Early Treatment of the Atopic Child (ETAC) population.
abstract: Many candidate gene studies for atopic dermatitis (AD) and associated phenotypes have been conducted so far, but replication of significant results has been a major problem. Two loss of function polymorphisms FLG R501X- and 2282del4, in the Filaggrin (FLG) gene encoding for an epidermal barrier protein were recently identified. They were reported to be predisposing factors for AD and concomitant asthma. Several groups confirmed the initial results in independent populations. The aim of this study is to further investigate the importance of these FLG variants in the development of AD and subsequent asthma symptoms in pre-school children, we investigated children and parents of the Early Treatment of the Atopic Child (ETAC)-trial. We genotyped 496 children and 488 parents of the ETAC population for the two FLG variants, evaluating an association by family based analysis (transmission disequilibrium test). We found a highly significant association of the FLG null variants R501X- and 2282del4 with AD (combined genotype p < 0.0001) and asthma (combined genotype p < 0.0001). The replication and its statistical significance underlines the importance of the FLG polymorphisms and the importance of the skin barrier function in the development of AD and subsequent asthma.
authors.affiliation: Department of Pediatric Pneumology and Immunology, Charité-Universitätsmedizin Berlin, Berlin, Germany.
authors.forename: Sebastian
authors.initials: S
authors.lastname: Müller
authors.forename: Ingo
authors.initials: I
authors.lastname: Marenholz
authors.forename: Young-Ae
authors.initials: YA
authors.lastname: Lee
authors.forename: Claudia
authors.initials: C
authors.lastname: Sengler
authors.forename: Simona Eva
authors.initials: SE
authors.lastname: Zitnik
authors.forename: Rupino W
authors.initials: RW
authors.lastname: Griffioen
authors.forename: Paolo
authors.initials: P
authors.lastname: Meglio
authors.forename: Ulrich
authors.initials: U
authors.lastname: Wahn
authors.forename: Renate
authors.initials: R
authors.lastname: Nickel
mesh_terms: D000483:Alleles; D000485:Allergens / Q000276:immunology; D001249:Asthma / Q000188:drug therapy / Q000235:genetics* / Q000276:immunology; D017332:Cetirizine / Q000627:therapeutic use; D003876:Dermatitis, Atopic / Q000188:drug therapy / Q000235:genetics* / Q000276:immunology; D004311:Double-Blind Method; D000091344:Filaggrin Proteins; D005500:Follow-Up Studies; D005787:Gene Frequency / Q000235:genetics / Q000276:immunology; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D007073:Immunoglobulin E / Q000097:blood; D007223:Infant; D007381:Intermediate Filament Proteins / Q000235:genetics* / Q000276:immunology; D009154:Mutation / Q000235:genetics; D016032:Randomized Controlled Trials as Topic
chemical_list: D000485:Allergens; C000717249:FLG protein, human; D000091344:Filaggrin Proteins; D007381:Intermediate Filament Proteins; D007073:Immunoglobulin E; D017332:Cetirizine