title: Cytogenetic and molecular identification of three Triticum aestivum-Leymus racemosus translocation addition lines.
abstract: Chromosome 2C from Aegilops cylindrica has the ability to induce chromosome breakage in common wheat (Tritivum aestivum). In the BC(1)F(3) generation of the T. aestivum cv. Chinese Spring and a hybrid between T. aestivum-Leymus racemosus Lr.7 addition line and T. aestivum-Ae. cylindrica 2C addition line, three disomic translocation addition lines (2n = 44) were selected by mitotic chromosome C-banding and genomic in situ hybridization. We further characterized these T. aestivum-L. racemosus translocation addition lines, NAU636, NAU637 and NAU638, by chromosome C-banding, in situ hybridization using the A- and D-genome-specific bacterial artificial chromosome (BAC) clones 676D4 and 9M13; plasmids pAs1 and pSc119.2, and 45S rDNA; as well as genomic DNA of L. racemosus as probes, in combination with double ditelosomic test cross and SSR marker analysis. The translocation chromosomes were designated as T3AS-Lr7S, T6BS-Lr7S, and T5DS-Lr7L. The translocation line T3AS-Lr7S was highly resistant to Fusarium head blight and will be useful germplasm for resistance breeding.
authors.affiliation: National Key Laboratory of Crop Genetics and Germplasm Enhancement, Cytogenetics Institute, Nanjing Agricultural University, Nanjing, China.
authors.forename: Le
authors.initials: L
authors.lastname: Wang
authors.forename: Jianhua
authors.initials: J
authors.lastname: Yuan
authors.forename: Tongde
authors.initials: T
authors.lastname: Bie
authors.forename: Bo
authors.initials: B
authors.lastname: Zhou
authors.forename: Peidu
authors.initials: P
authors.lastname: Chen
mesh_terms: D002678:Chimera / Q000235:genetics*; D002871:Chromosome Banding; D032461:Chromosomes, Plant / Q000235:genetics; D003582:Cytogenetics; D006824:Hybridization, Genetic; D006109:Poaceae / Q000235:genetics*; D014178:Translocation, Genetic; D014908:Triticum / Q000235:genetics*