title: Identification of antigenic Brugia adult worm proteins by peptide mass fingerprinting.
abstract: With the recent completion of the Brugia malayi genome, proteomics offers a new resource for a deeper understanding of the biology of filarial parasites. We employed 2-dimensional (2D) gel electrophoresis followed by peptide mass fingerprinting on a matrix-assisted laser desorption/ionization time-of-flight (MALDI-ToF) mass spectrometer to identify Brugia adult worm proteins and then determined which proteins were recognized by the host humoral immune response. We identified 18 unique proteins, several of which were determined to be antigenic by immunoblot. The proteins identified here may contribute to future studies to analyze the transmission and pathogenesis of lymphatic filariasis.
authors.affiliation: Department of Cell Biology, 724 Biological Sciences Building, University of Georgia, Athens, Georgia 30602, USA.
authors.forename: Tiffany
authors.initials: T
authors.lastname: Weinkopff
authors.forename: James A
authors.initials: JA
authors.lastname: Atwood
authors.forename: George A
authors.initials: GA
authors.lastname: Punkosdy
authors.forename: Delynn
authors.initials: D
authors.lastname: Moss
authors.forename: D Brent
authors.initials: DB
authors.lastname: Weatherly
authors.forename: Ron
authors.initials: R
authors.lastname: Orlando
authors.forename: Patrick
authors.initials: P
authors.lastname: Lammie
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood; D000947:Antigens, Helminth / Q000032:analysis* / Q000737:chemistry / Q000276:immunology; D017179:Brugia pahangi / Q000737:chemistry* / Q000276:immunology; D015180:Electrophoresis, Gel, Two-Dimensional; D004591:Electrophoresis, Polyacrylamide Gel; D004605:Elephantiasis, Filarial / Q000097:blood / Q000276:immunology / Q000469:parasitology; D005849:Gerbillinae; D015801:Helminth Proteins / Q000032:analysis* / Q000737:chemistry / Q000276:immunology; D006801:Humans; D007091:Image Processing, Computer-Assisted; D015151:Immunoblotting; D008969:Molecular Sequence Data; D040901:Proteomics; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D015801:Helminth Proteins