title: Label-free detection of specific DNA sequence-telomere using unmodified gold nanoparticles as colorimetric probes.
abstract: A simple and sensitive label-free colorimetric detection of telomere DNA has been developed. It was based on the color change of gold nanoparticles (AuNPs) due to DNA hybridization. UV-vis spectra and transmission electron microscopy (TEM) were used to investigate the change of AuNPs. Under the optimized conditions, the linear range for determination of telomere DNA was 5.7 x 10(-13) to 4.5 x 10(-6)mol/L. The detection limit (3 sigma) of this method has decreased to pico-molar level.
authors.affiliation: Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry and Materials Science, Shaanxi Normal University, Xi'an 710062, China.
authors.forename: Yingying
authors.initials: Y
authors.lastname: Qi
authors.forename: Li
authors.initials: L
authors.lastname: Li
authors.forename: Baoxin
authors.initials: B
authors.lastname: Li
mesh_terms: D001483:Base Sequence; D015374:Biosensing Techniques / Q000379:methods; D003124:Colorimetry / Q000379:methods; D004247:DNA / Q000032:analysis*; D004526:Efficiency; D006046:Gold / Q000737:chemistry / Q000494:pharmacology*; D053768:Metal Nanoparticles / Q000737:chemistry; D008954:Models, Biological; D009693:Nucleic Acid Hybridization / Q000187:drug effects; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet; D013194:Staining and Labeling / Q000379:methods*; D016615:Telomere / Q000737:chemistry*
chemical_list: D006046:Gold; D004247:DNA