title: Dynamic disorder in horseradish peroxidase observed with total internal reflection fluorescence correlation spectroscopy.
abstract: This paper discusses the application of objective-type total internal reflection fluorescence correlation spectroscopy (TIR-FCS) to the study of the kinetics of immobilized horseradish peroxidase on a single molecule level. Objective-type TIR-FCS combines the advantages of FCS with TIRF microscopy in a way that allows for simultaneous ultra-sensitive spectroscopic measurements using a single-point detector and convenient localization of single molecules on a surface by means of parallel imaging.
authors.forename: Kai
authors.initials: K
authors.lastname: Hassler
authors.forename: Per
authors.initials: P
authors.lastname: Rigler
authors.forename: Hans
authors.initials: H
authors.lastname: Blom
authors.forename: Rudolf
authors.initials: R
authors.lastname: Rigler
authors.forename: Jerker
authors.initials: J
authors.lastname: Widengren
authors.forename: Theo
authors.initials: T
authors.lastname: Lasser