title: Characterization of electrogenerated polypyrrole-benzophenone films coated on poly(pyrrole-methyl metacrylate) optic-conductive fibers.
abstract: A conductive surface was created for the development of a biosensing platform via chemical polymerization of pyrrole onto the surface of poly(methyl methacrylate) (PMMA) fibers, with a subsequent electrogeneration of a photoactive linker pyrrole-benzophenone (PyBz) monomer on the fiber surface. Irradiation of the benzophenone groups embedded in the polypyrrole (Ppy) films by UV (350 nm) formed active radicals, allowing covalent attachment of the desired biomaterials. Characterization and optimization of this platform were carried out, with the platform showing conductive, stable, thin, controllable, and light-transmissible film features. Various parameters such as time deposition, process temperature, and activator plus pyrrole monomer concentrations were examined in the study. The morphology and permeability of the optic-fiber PMMA fibers were investigated to examine mass transfer ability. Cyclic voltammetry and amperometry techniques were applied to characterize the electrical features of the surface and charge transfer. The platform potential was then demonstrated by the construction of both amperometric and optical biosensors.
authors.affiliation: Department of Biotechnology Engineering. Ben-Gurion University of the Negev, P.O. Box 653, Beer-Sheva 84105, Israel.
authors.forename: Khalil
authors.initials: K
authors.lastname: Abu-Rabeah
authors.forename: Danit
authors.initials: D
authors.lastname: Atias
authors.forename: Sebastien
authors.initials: S
authors.lastname: Herrmann
authors.forename: Julia
authors.initials: J
authors.lastname: Frenkel
authors.forename: Dorith
authors.initials: D
authors.lastname: Tavor
authors.forename: Serge
authors.initials: S
authors.lastname: Cosnier
authors.forename: Robert S
authors.initials: RS
authors.lastname: Marks
mesh_terms: D001577:Benzophenones / Q000737:chemistry*; D015374:Biosensing Techniques; D002384:Catalysis; D004553:Electric Conductivity; D004563:Electrochemistry / Q000379:methods*; D008027:Light; D008855:Microscopy, Electron, Scanning / Q000379:methods; D055100:Optical Fibers; D055095:Optics and Photonics; D010539:Permeability; D019904:Polymethyl Methacrylate / Q000737:chemistry*; D011758:Pyrroles / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared; D013696:Temperature
chemical_list: D001577:Benzophenones; D011758:Pyrroles; C047723:benzophenone; D019904:Polymethyl Methacrylate