AFM phase imaging of soft-hydrated samples: a versatile tool to complete the chemical-physical study of liposomes.
Despite of the several approaches applied to the physicochemical characterization of liposomes, few techniques are really useful to obtain information about the surface properties of these colloidal drug-delivery systems. In this paper, we demonstrate a possible new application of tapping mode atomic force microscopy (AFM) to discriminate between conventional and pegylated liposomes. We showed that the differences on liposomal surface properties revealed by the phase images AFM approach well correlate with the data obtained using classical methods, such as light scattering, hydrodynamic, and nuclear magnetic resonance analysis.
Ruozi
Barbara
B
Department of Pharmaceutical Science, University of Modena and Reggio Emilia, Modena, Italy.
Tosi
Giovanni
G
Tonelli
Massimo
M
Bondioli
Lucia
L
Mucci
Adele
A
Forni
Flavio
F
Vandelli
Maria Angela
MA
D008081:Liposomes / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D018625:Microscopy, Atomic Force / Q000379:methods*; D011092:Polyethylene Glycols / Q000737:chemistry*; D013499:Surface Properties
D008081:Liposomes; D011092:Polyethylene Glycols