Fast separation of microcystins and nodularins on narrow-bore reversed-phase columns coupled to a conventional HPLC system.
Six modern narrow-bore (50 mm length x 2-2.1 mm internal diameter) reversed-phase HPLC columns were tested in the rapid separation of ten microcystins and three nodularins, cyanobacterial peptide hepatotoxins. The columns had either a sub-3 microm particle size or were of monolithic silica technology for high efficiency and rapid run times. A standard low-pressure gradient HPLC equipment with diode-array UV detector was used for the chromatography. The gradient mobile phase consisted of water and acetonitrile, both acidified with trifluoroacetic acid, and the gradient rise times were 1-4 min. Chromatographic performance was assessed by visual judgement and by calculating parameters such as capacity factors, resolution, peak width, selectivity and peak form. Several chromatographic conditions produced excellent results. We recommend sub-3 microm particle-based or monolithic reversed-phase columns for routine use in microcystin and nodularin HPLC analyses.
Spoof
Lisa
L
Department of Biochemistry and Pharmacy, Abo Akademi University, Tykistökatu 6A, 20520 Turku, Finland.
Neffling
Milla-Riina
MR
Meriluoto
Jussi
J
D001427:Bacterial Toxins / Q000302:isolation & purification* / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D000458:Cyanobacteria / Q000378:metabolism*; D000087522:Cyanobacteria Toxins; D008387:Marine Toxins / Q000302:isolation & purification* / Q000378:metabolism; D052998:Microcystins / Q000302:isolation & purification* / Q000378:metabolism; D010456:Peptides, Cyclic / Q000302:isolation & purification* / Q000378:metabolism
D001427:Bacterial Toxins; D000087522:Cyanobacteria Toxins; D008387:Marine Toxins; D052998:Microcystins; D010456:Peptides, Cyclic; C063998:nodularin