Comparison of the effect of multipurpose contact lens solutions on the viability of cultured corneal epithelial cells.
OBJECTIVE
To determine the effect of four marketed multipurpose contact lens solutions (MPSs) on corneal epithelial cell viability.


METHODS
Comparison of the effect of MPS A (Renu MultiPlus, Bausch & Lomb), MPS B (OPTI-FREE Express, Alcon), MPS C (AQuify, CibaVision), and MPS D (OPTI-FREE RepleniSH, Alcon) on cell viability was performed by quantifying cellular ATP content, resazurin reduction, and lactate dehydrogenase (LDH) release in transformed human corneal epithelial cells (HCEpiC) and primary bovine corneal epithelial cells (BCEpiC).


RESULTS
Significant reductions in cellular ATP content were observed at 40% solution and above with both MPS B and MPS D, compared to at 100% only for MPS A and MPS C, and similar results were obtained in BCEpiC. Effects on resazurin reduction were also less in HCEpiC exposed to increasing doses of MPS A and MPS C than in cells exposed to MPS B and MPS D. After 15 min, HCEpiC viability measured by both resazurin reduction and cellular ATP levels was significantly lower for cells exposed to MPS B, MPS D, and MPS C, while HCEpiC exposed to MPS A were not affected. MPS B and MPS D reduced cell viability more than MPS A and MPS C over a 2-h time course in both HCEpiC and BCEpiC.


CONCLUSIONS
Both MPS B and MPS D can cause large decreases in the viability of cultured corneal epithelial cells even with just a 2h exposure at multiple doses. Significant reduction in cell viability is evident at brief 15-30 min exposures. In contrast, MPS A and MPS C have significantly less effect on the cell viability of corneal epithelial cells at multiple doses, after these short exposure times.
Cavet
Megan E
ME
Pharmaceutical R&D, Bausch & Lomb, 1400 North Goodman Street, Rochester, NY 14609, USA. megan.cavet@bausch.com
Harrington
Karen L
KL
VanDerMeid
Karl R
KR
Ward
Keith W
KW
Zhang
Jin-Zhong
JZ
D000255:Adenosine Triphosphate / Q000032:analysis; D000818:Animals; D000890:Anti-Infective Agents / Q000008:administration & dosage; D002417:Cattle; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D018639:Contact Lens Solutions / Q000737:chemistry / Q000494:pharmacology*; D004847:Epithelial Cells / Q000737:chemistry / Q000187:drug effects* / Q000378:metabolism / Q000502:physiology*; D019573:Epithelium, Corneal / Q000737:chemistry / Q000166:cytology* / Q000378:metabolism; D006801:Humans; D066298:In Vitro Techniques; D007770:L-Lactate Dehydrogenase / Q000032:analysis; D010078:Oxazines / Q000378:metabolism; D010084:Oxidation-Reduction / Q000187:drug effects; D013997:Time Factors; D014966:Xanthenes / Q000378:metabolism
D000890:Anti-Infective Agents; D018639:Contact Lens Solutions; D010078:Oxazines; D014966:Xanthenes; C005843:resazurin; D000255:Adenosine Triphosphate; D007770:L-Lactate Dehydrogenase