Using Ruditapes philippinarum conglutination mud to produce bioflocculant and its applications in wastewater treatment.
A novel bioflocculant-producing bacterium, ZHT4-13, was isolated from Ruditapes philippinarum conglutination mud. By biomicroscope morphological observation, 16S rDNA sequence identification and physiological and biochemical characteristics, strain ZHT4-13 was identified as Rothia sp. The bioflocculant MBF4-13 produced by strain ZHT4-13 had a flocculating efficiency of 86.22% for 5 g L(-1) Kaolin clay suspension when the initial pH was 9 and the temperature was 20 degrees C. It had flocculating effect in a wide range, pH 1-13 and temperature 4-100 degrees C. Analysis of MBF4-13 by UV-Vis spectrophotometer, Fourier-transform infrared spectrophotometer (FT-IR) and (1)H nuclear magnetic resonance (NMR) indicated that the main component of MBF4-13 is polysaccharide. The culture conditions to produce strain ZHT4-13 were optimized with orthogonal design of experiments. MBF4-13 had high efficiency in decolorizing dye solutions, had some abilities to remove heavy metal ions (Cr(2)O(7)(2-), Ni(2+)) and improve performance of activated sludge.
Gao
Qi
Q
School of Environmental and Chemical Engineering, Dalian Jiaotong University, Dalian 116028, China.
Zhu
Xiu-Hua
XH
Mu
Jun
J
Zhang
Yi
Y
Dong
Xue-Wei
XW
D000818:Animals; D001419:Bacteria / Q000166:cytology / Q000302:isolation & purification; D001673:Biodegradation, Environmental; D049872:Bivalvia / Q000382:microbiology*; D003116:Color; D004396:Coloring Agents / Q000737:chemistry; D005427:Flocculation; D005658:Fungi / Q000166:cytology; D006863:Hydrogen-Ion Concentration; D007477:Ions; D007616:Kaolin / Q000737:chemistry; D019216:Metals, Heavy / Q000302:isolation & purification; D016133:Polymerase Chain Reaction; D012722:Sewage / Q000382:microbiology; D012988:Soil Microbiology; D012996:Solutions; D013696:Temperature; D014865:Waste Disposal, Fluid; D018508:Water Purification
D004396:Coloring Agents; D007477:Ions; D019216:Metals, Heavy; D012722:Sewage; D012996:Solutions; D007616:Kaolin