Periodontal wound healing/regeneration following implantation of recombinant human growth/differentiation factor-5 (rhGDF-5) in an absorbable collagen sponge carrier into one-wall intrabony defects in dogs: a dose-range study.
OBJECTIVE
Recombinant human growth/differentiation factor-5 (rhGDF-5) is being evaluated as a candidate therapy in support of periodontal regeneration. The objective of this study was to evaluate cementum and alveolar bone formation, and aberrant healing events following surgical implantation of rhGDF-5 in an absorbable collagen sponge (ACS) carrier using an established periodontal defect model.


METHODS
Bilateral 4 x 5 mm (width x depth), one-wall, critical-size, intrabony periodontal defects were surgically created at the mandibular second and fourth pre-molar teeth in 15 Beagle dogs. Five animals received 1 microg/defect and five animals 20 microg/defect rhGDF-5 in unilateral defect sites. Contralateral sites received treatments reported elsewhere. Five animals received rhGDF-5/ACS with 0 (buffer control) and 100 microg/defect rhGDF-5 in contralateral defect sites. The animals were euthanized at 8 weeks post-surgery for histologic and histometric evaluation.


RESULTS
Surgical implantation of rhGDF-5 stimulated significant periodontal regeneration. Cementum formation was significantly enhanced in sites implanted with rhGDF-5 (1 and 100 microg) compared with control (p<0.05). Similarly, bone formation height was significantly greater in sites receiving rhGDF-5 (1 and 100 microg) compared with control (p<0.05). There were no significant or remarkable differences in bone and cementum formation within the selected dose interval (1, 20 and 100 microg rhGDF-5). None of the control or the rhGDF-5 sites exhibited root resorption, ankylosis, or other aberrant tissue reactions.


CONCLUSIONS
Surgical implantation of rhGDF-5/ACS may be used safely to support periodontal wound healing/regeneration in intrabony periodontal defects without complications.
Kim
Tae-Gyun
TG
Department of Periodontology, Research Institute for Periodontal Regeneration, College of Dentistry, Yonsei University, Seoul, Korea.
Wikesjö
Ulf M E
UM
Cho
Kyoo-Sung
KS
Chai
Jung-Kiu
JK
Pippig
Susanne D
SD
Siedler
Michael
M
Kim
Chong-Kwan
CK
D020341:Absorbable Implants; D000539:Alveolar Process / Q000187:drug effects* / Q000601:surgery; D000704:Analysis of Variance; D000818:Animals; D001861:Bone Regeneration / Q000187:drug effects* / Q000502:physiology; D003094:Collagen; D003739:Dental Cementum / Q000187:drug effects* / Q000601:surgery; D004285:Dogs; D004305:Dose-Response Relationship, Drug; D055428:Growth Differentiation Factor 5 / Q000008:administration & dosage* / Q000502:physiology; D006801:Humans; D008297:Male; D008334:Mandible; D010513:Periodontal Ligament / Q000187:drug effects* / Q000502:physiology; D011994:Recombinant Proteins / Q000008:administration & dosage; D016037:Single-Blind Method; D015503:Surgical Sponges; D014945:Wound Healing / Q000187:drug effects / Q000502:physiology
D055428:Growth Differentiation Factor 5; D011994:Recombinant Proteins; D003094:Collagen