title: Sequence analysis of the large (L) polymerase gene and trailer of the peste des petits ruminants virus vaccine strain Nigeria 75/1: expression and use of the L protein in reverse genetics.
abstract: The large (L) polymerase gene and the 5'-terminal UTR of the genome of peste des petits ruminants virus (PPRV), vaccine strain Nigeria 75/1, were cloned and sequenced. The L protein was also expressed in eukaryotic cells and its polymerase activity was quantitatively measured in a PPR reverse genetics assay using a reporter minigenome. Comparative sequence analysis of this functional L gene with corresponding genes of other morbilliviruses showed a degree of conservation exceeding 70%. The multiple sequence alignment and the phylogenetic study of L gene discriminated the morbilliviruses in 6 clusters, which are more closely related to Tupaia and Henipaviruses than to other paramyxoviruses. Important protein domains and functional motifs of the L polymerase of the PPRV Nigeria 75/1 vaccine were also identified by using different bioinformatics tools.
authors.affiliation: CIRAD, UMR CIRAD/INRA Contrôle des Maladies, Montpellier, France.
authors.forename: C
authors.initials: C
authors.lastname: Minet
authors.forename: M
authors.initials: M
authors.lastname: Yami
authors.forename: B
authors.initials: B
authors.lastname: Egzabhier
authors.forename: P
authors.initials: P
authors.lastname: Gil
authors.forename: F
authors.initials: F
authors.lastname: Tangy
authors.forename: M
authors.initials: M
authors.lastname: Brémont
authors.forename: G
authors.initials: G
authors.lastname: Libeau
authors.forename: A
authors.initials: A
authors.lastname: Diallo
authors.forename: E
authors.initials: E
authors.lastname: Albina
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002522:Chlorocebus aethiops; D012321:DNA-Directed RNA Polymerases / Q000096:biosynthesis / Q000235:genetics; D005814:Genes, Viral; D016679:Genome, Viral; D008969:Molecular Sequence Data; D029021:Peste-des-Petits-Ruminants / Q000378:metabolism / Q000821:virology*; D018107:Peste-des-petits-ruminants virus / Q000235:genetics* / Q000378:metabolism; D010802:Phylogeny; D012367:RNA, Viral / Q000032:analysis / Q000235:genetics; D016415:Sequence Alignment; D017423:Sequence Analysis, RNA; D014709:Vero Cells; D014764:Viral Proteins / Q000235:genetics*
chemical_list: D012367:RNA, Viral; D014764:Viral Proteins; D012321:DNA-Directed RNA Polymerases