Surface coating with a thermoresponsive copolymer for the culture and non-enzymatic recovery of mouse embryonic stem cells.
A thermoresponsive substrate based on a triblock copolymer, poly(N-isopropylacrylamide)-block-poly[(R)-3-hydroxybutyrate]-block-poly(N-isopropylacrylamide) (PNIPAAm-PHB-PNIPAAm), co-coated with gelatin, was developed for the culture and non-enzymatic recovery of mouse embryonic stem cells. After culture, the cells could be detached by cooling at 4 degrees C for 20 min without trypsin digestion. The embryonic stem cells remained undifferentiated after culture on the gelatin/copolymer-coated surfaces, similar to standard culture techniques. Overall, the triblock copolymer coating was superior to the PNIPAAm homopolymer coating in terms of supporting better cell growth, being more stable, presenting a more homogeneous surface coating, and maintaining pluripotency of the embryonic stem cells.
Loh
Xian Jun
XJ
Nano Medical Engineering Laboratory, RIKEN Advanced Science Institute, 2-1 Hirosawa, Wako-shi, Saitama 351-0198, Japan.
Gong
Jiansheng
J
Sakuragi
Makoto
M
Kitajima
Takashi
T
Liu
Mingzhe
M
Li
Jun
J
Ito
Yoshihiro
Y
D000818:Animals; D002448:Cell Adhesion; D018929:Cell Culture Techniques / Q000379:methods*; D002454:Cell Differentiation; D048430:Cell Shape; D002478:Cells, Cultured; D020099:Coated Materials, Biocompatible / Q000737:chemistry; D053595:Embryonic Stem Cells / Q000166:cytology* / Q000502:physiology; D008422:Materials Testing; D051379:Mice; D015394:Molecular Structure; D011108:Polymers / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared; D013499:Surface Properties; D013696:Temperature
D020099:Coated Materials, Biocompatible; D011108:Polymers