[SiRNA-mediated silencing of 5-lipoxygenase gene (ALOX5) reduces necrosis of neonatal cardiomyocytes in anoxia-reoxygenation].
In experiments on the primary culture of isolated neonatal rat cardiomyocytes it was determined that cardiomyocytes express ALOX5 gene encoding enzyme 5-lipoxygenase. Anoxia-reoxygenation does not affect significantly the expression of 5-lipoxygenase mRNA in cardiomyocytes. Transfection of 5-lipoxygenase-specific small interfering RNA's (siRNA) into cardiomyocytes lead to a significant reduction of 5-lipoxygenase mRNA expression in cardiomyocytes 24 hours after transfection. ALOX5 gene silencing resulted in improved viability of cell population (by 13.3% P < 0.001) due to decreased number of necrotic (by 14.6%, P < 0.001), but not apoptotic, cells during anoxia-reoxygenation. Our results indicate that siRNA against ALOX5 effectively protects cardiomyocytes against anoxia-reoxygenation injury.
Lisovyĭ
O O
OO
Nahibin
V S
VS
Surova
O V
OV
Tumanovs'ka
L V
LV
Dosenko
B Ie
BIe
Moĭbenko
O O
OO
D000818:Animals; D000831:Animals, Newborn; D017209:Apoptosis; D001094:Arachidonate 5-Lipoxygenase / Q000235:genetics*; D015687:Cell Hypoxia; D002478:Cells, Cultured; D020868:Gene Silencing; D032383:Myocytes, Cardiac / Q000201:enzymology* / Q000378:metabolism / Q000473:pathology*; D009336:Necrosis; D010100:Oxygen / Q000378:metabolism*; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000494:pharmacology*; D051381:Rats
D034741:RNA, Small Interfering; D001094:Arachidonate 5-Lipoxygenase; D010100:Oxygen