title: Expression and function of intestinal hexose transporters after small intestinal denervation.
abstract: BACKGROUND
  The role of neural regulation in expression and function of intestinal hexose transporters is unknown. The aim of this study is to determine the role of intestinal innervation in gene expression and function of the membrane hexose transporters, SGLT1, GLUT2, and GLUT5 in the enterocyte. We hypothesize that denervation of the small intestine decreases expression of hexose transporters, which leads to decreased glucose absorption.
  
  
  METHODS
  Six groups of Lewis rats were studied (n = 6 each) as follows: control, 1 week after sham laparotomy, 1 and 8 weeks after syngeneic (no immune rejection) orthotopic small-bowel transplantation (SBT) (SBT1 and SBT8) to induce complete extrinsic denervation, and 1 and 8 weeks after selective disruption of intrinsic neural continuity to jejunoileum by gut transection and reanastomosis (T/A1 and T/A8). All tissue was harvested between 8 AM and 10 AM. In duodenum, jejunum, and ileum, mucosal messenger RNA (mRNA) levels were quantitated by real-time polymerase chain reaction (PCR), protein by Western blotting, and transporter-mediated glucose absorption using the everted sleeve technique.
  
  
  RESULTS
  Across the 6 groups, the relative gene expression of hexose transporter mRNA and protein levels were unchanged, and no difference in transporter-mediated glucose uptake was evident in any region. The glucose transporter affinity (K(m)) and functional transporter levels (V(max)) calculated for duodenum and jejunum showed no difference among the 6 groups.
  
  
  CONCLUSIONS
  Baseline regulation of hexose transporter function is not mediated tonically by intrinsic or extrinsic neural continuity to the jejunoileum.
authors.affiliation: Department of Surgery, Mayo Clinic College of Medicine, Rochester, MN 55905, USA.
authors.forename: Corey W
authors.initials: CW
authors.lastname: Iqbal
authors.forename: Javairiah
authors.initials: J
authors.lastname: Fatima
authors.forename: Judith
authors.initials: J
authors.lastname: Duenes
authors.forename: Scott G
authors.initials: SG
authors.lastname: Houghton
authors.forename: Michael S
authors.initials: MS
authors.lastname: Kasparek
authors.forename: Michael G
authors.initials: MG
authors.lastname: Sarr
mesh_terms: D000818:Animals; D003714:Denervation; D004386:Duodenum / Q000294:innervation / Q000378:metabolism / Q000637:transplantation; D005786:Gene Expression Regulation / Q000502:physiology; D051273:Glucose Transporter Type 2 / Q000235:genetics / Q000378:metabolism*; D051296:Glucose Transporter Type 5 / Q000235:genetics / Q000378:metabolism*; D007082:Ileum / Q000294:innervation / Q000378:metabolism / Q000637:transplantation; D007421:Intestine, Small / Q000294:innervation* / Q000378:metabolism* / Q000637:transplantation; D007583:Jejunum / Q000294:innervation / Q000378:metabolism / Q000637:transplantation; D008297:Male; D023421:Models, Animal; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D011917:Rats, Inbred Lew; D051269:Sodium-Glucose Transporter 1 / Q000235:genetics / Q000378:metabolism*
chemical_list: D051273:Glucose Transporter Type 2; D051296:Glucose Transporter Type 5; D012333:RNA, Messenger; C495683:Slc2a2 protein, rat; C495727:Slc2a5 protein, rat; C495676:Slc5a1 protein, rat; D051269:Sodium-Glucose Transporter 1