title: A llama single domain anti-idiotypic antibody mimicking HER2 as a vaccine: Immunogenicity and efficacy.
abstract: HER2 over-expression in breast cancer correlates with reduced survival. Anti-idiotypic antibodies (Abs) that closely mimic HER2 could play a crucial role in the development of effective therapeutic vaccines. Here, we show that an anti-idiotypic single domain antibody (sdAb) 1HE isolated from a library generated from a Trastuzumab F(ab')(2)-immunized llama, closely mimics HER2. SdAb 1HE shows high affinity for Trastuzumab F(ab')(2), selectively inhibits HER2 binding to Trastuzumab F(ab')(2), and sera from sdAb 1HE-immunized BALB/c mice contain anti-HER2 antibodies that inhibit viability of HER2-positive cells. These results indicate that sdAb 1HE could be used as an anti-idiotype-based vaccine to boost immunity in patients bearing HER2-positive tumours.
authors.affiliation: IRCM (Institut de Recherche en Cancérologie de Montpellier), INSERM U896, Université Montpellier 1, CRLC Val d'Aurelle Paul Lamarque, Montpellier Cedex 5, France.
authors.forename: Nidia
authors.initials: N
authors.lastname: Alvarez-Rueda
authors.forename: Maha Zohra
authors.initials: MZ
authors.lastname: Ladjemi
authors.forename: Ghislaine
authors.initials: G
authors.lastname: Béhar
authors.forename: Stéphanie
authors.initials: S
authors.lastname: Corgnac
authors.forename: Martine
authors.initials: M
authors.lastname: Pugnière
authors.forename: Françoise
authors.initials: F
authors.lastname: Roquet
authors.forename: Caroline
authors.initials: C
authors.lastname: Bascoul-Mollevi
authors.forename: Daniel
authors.initials: D
authors.lastname: Baty
authors.forename: André
authors.initials: A
authors.lastname: Pèlegrin
authors.forename: Isabelle
authors.initials: I
authors.lastname: Navarro-Teulon
mesh_terms: D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000276:immunology* / Q000302:isolation & purification; D000911:Antibodies, Monoclonal / Q000276:immunology; D061067:Antibodies, Monoclonal, Humanized; D002161:Camelids, New World / Q000276:immunology*; D019496:Cancer Vaccines / Q000276:immunology*; D045744:Cell Line, Tumor; D002470:Cell Survival; D005260:Female; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D010063:Ovum / Q000276:immunology / Q000502:physiology; D019151:Peptide Library; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000037:antagonists & inhibitors*; D000068878:Trastuzumab
chemical_list: D000888:Antibodies, Anti-Idiotypic; D000911:Antibodies, Monoclonal; D061067:Antibodies, Monoclonal, Humanized; D019496:Cancer Vaccines; D019151:Peptide Library; D018719:Erb-b2 Receptor Tyrosine Kinases; D000068878:Trastuzumab; C524056:Erbb2 protein, mouse