title: Donor variation in in vitro HIV-1 susceptibility of monocyte-derived macrophages.
abstract: Primary human cells from different donors vary in their susceptibility to in vitro infection with HIV-1. In order to perform genetic analysis to identify host factors that affect HIV-1 susceptibility, it is important that a clear phenotype is defined. Here, we report a standardized method to study variation for in vitro HIV-1 infection in monocyte-derived macrophages (MDM) from large numbers of individuals. With this assay, HIV-1 susceptibility of MDM from 489 different donors shows more than 3 log variation and a good correlation with the 32 base pair deletion in the CCR5 co-receptor (ccr5 Delta 32 genotype) of the donors. However, in 7 of 12 donors completely resistant to infection with CCR5-using HIV-1, this was not explained by the ccr5 Delta 32 genotype, showing evidence that other host factors are likely to influence HIV-1 replication in MDM. Infections with VSV-G pseudotyped HIV-1 indeed confirmed the existence of post-entry level restrictions in MDM.
authors.affiliation: Department of Experimental Immunology, Sanquin Research, Landsteiner Laboratory and Center for Infectious Diseases and Immunity Amsterdam (CINIMA), Academic Medical Center of The University of Amsterdam, Amsterdam, The Netherlands.
authors.forename: Sebastiaan M
authors.initials: SM
authors.lastname: Bol
authors.forename: Yvonne
authors.initials: Y
authors.lastname: van Remmerden
authors.forename: Jantine G
authors.initials: JG
authors.lastname: Sietzema
authors.forename: Neeltje A
authors.initials: NA
authors.lastname: Kootstra
authors.forename: Hanneke
authors.initials: H
authors.lastname: Schuitemaker
authors.forename: Angélique B
authors.initials: AB
authors.lastname: van't Wout
mesh_terms: D000328:Adult; D002478:Cells, Cultured; D005260:Female; D020022:Genetic Predisposition to Disease; D014644:Genetic Variation; D015497:HIV-1 / Q000254:growth & development* / Q000276:immunology; D006801:Humans; D008264:Macrophages / Q000276:immunology / Q000821:virology*; D008297:Male; D008875:Middle Aged; D019713:Receptors, CCR5 / Q000235:genetics; D017384:Sequence Deletion; D055815:Young Adult
chemical_list: D019713:Receptors, CCR5