Root canal filling with cements based on mineral aggregates: an in vitro analysis of bacterial microleakage.
The aim of this study was to assess bacterial microleakage through 2 different cements used as root canal filling materials: mineral-trioxide-aggregate (White MTA, Angelus, Londrina, PR, Brazil); and an experimental material containing calcium aluminate plus additives (EndoBinder, University of São Carlos [UFSCar-Brazil], patent number PI0704502-6). Forty incisors were divided into 4 groups: (1) white-MTA, (2) calcium aluminate cement, and (3) positive and (4) negative control group. Falcon tubes were prepared for this experiment, divided into 2 separated chambers; the lower part was filled with RTF-transport media so that only the root apex was in contact with the transport media, whereas the coronal part was immersed in BHI containing Enterococcus faecalis to investigate bacterial microleakage. The evaluated materials used as root canal filling materials showed an absence of microbial growth. Both materials when used as the root canal filling were efficient in sealing root canals and preventing E. faecalis microleakage for the 30-day evaluation period using an in vitro model.
Jacobovitz
Marcos
M
Department of Endodontics, Hadassa Dental Medicine School, Hebrew University of Jerusalem, Jerusalem, Israel.
Vianna
Morgana E
ME
Pandolfelli
Victor C
VC
Oliveira
Ivone R
IR
Rossetto
Hebert L
HL
Gomes
Brenda P F A
BP
D017607:Aluminum Compounds / Q000737:chemistry*; D017610:Calcium Compounds / Q000737:chemistry*; D015169:Colony Count, Microbial; D003738:Dental Cements / Q000737:chemistry*; D003763:Dental Leakage / Q000382:microbiology*; D003786:Dental Pulp Cavity / Q000382:microbiology*; D004338:Drug Combinations; D004492:Edetic Acid / Q000627:therapeutic use; D013293:Enterococcus faecalis / Q000254:growth & development*; D006801:Humans; D006813:Humidity; D008422:Materials Testing; D010087:Oxides / Q000737:chemistry; D012387:Root Canal Filling Materials / Q000737:chemistry*; D012388:Root Canal Irrigants / Q000627:therapeutic use; D018915:Root Canal Preparation / Q000295:instrumentation / Q000379:methods; D017640:Silicates / Q000737:chemistry; D012973:Sodium Hypochlorite / Q000627:therapeutic use; D013696:Temperature; D013997:Time Factors; D019227:Tooth Apex / Q000382:microbiology
D017607:Aluminum Compounds; D017610:Calcium Compounds; D003738:Dental Cements; D004338:Drug Combinations; C557697:EndoBinder; D010087:Oxides; D012387:Root Canal Filling Materials; D012388:Root Canal Irrigants; D017640:Silicates; C086631:mineral trioxide aggregate; C035219:calcium aluminate; D004492:Edetic Acid; D012973:Sodium Hypochlorite