Lidocaine priming reduces ADP-induced P-selectin expression and platelet-leukocyte aggregation.
BACKGROUND
Activation of platelets, which plays an important role in inflammation, has recently been reported to enhance platelet P-selectin expression and form platelet-leukocyte aggregation (PLA). Platelet P-selectin expression and PLA formation have been reported to be potential markers of inflammatory diseases such as sepsis, thrombosis, myocardial ischemic disorders and stroke. Lidocaine, one of the most commonly used anesthetics, is known to inhibit platelet function, but its effect on platelet P-selectin expression and PLA remains unclear.


METHODS
To determine the effect of lidocaine on platelet activation, and on platelet activation-related septic condition (lipopolysaccharide-induced), we treated platelets with lidocaine (0.03-3 mM) and then measured platelet P-selectin expression and PLA. Whole blood for in vitro study was obtained from healthy men aged 27 to 33 years who had not taken any medication for at least 15 days.


RESULTS
All samples were analyzed by flow cytometry. We found that lidocaine produced a concentration-dependent inhibition of P-selectin expression and PLA. Moreover, in lipopolysaccharide-challenged samples, lidocaine at concentrations of 1-3 mM inhibited PLA.


CONCLUSIONS
Our findings may help to elucidate the inhibitory role of lidocaine on platelet P-selectin expression and PLA and infer possible therapeutic targets in the treatment of inflammatory diseases. However, further investigations are needed to determine whether the observed attenuation of excessive inflammatory responses has clinical implications. These results suggest that lidocaine might have potential clinical application in the modulation of excessive platelet activation, inflammatory response and septic condition.
Huang
Go-Shine
GS
Department of Anesthesiology, Tri-Service General Hospital, National Defense Medical Center, Taipei, Taiwan, R.O.C.
Lin
Tso-Chou
TC
Wang
Jia-Yi
JY
Ku
Chih-Hung
CH
Ho
Shung-Tai
ST
Li
Chi-Yuan
CY
D000244:Adenosine Diphosphate / Q000494:pharmacology*; D000328:Adult; D000779:Anesthetics, Local / Q000494:pharmacology*; D001792:Blood Platelets / Q000187:drug effects* / Q000502:physiology; D002118:Calcium / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D006801:Humans; D007962:Leukocytes / Q000187:drug effects* / Q000502:physiology; D008012:Lidocaine / Q000494:pharmacology*; D008070:Lipopolysaccharides / Q000633:toxicity; D019007:P-Selectin / Q000097:blood*; D010974:Platelet Aggregation / Q000187:drug effects*
D000779:Anesthetics, Local; D008070:Lipopolysaccharides; D019007:P-Selectin; D000244:Adenosine Diphosphate; D008012:Lidocaine; D002118:Calcium