The local lymph node assay compared with the human maximization test as an indicator of allergic potency in humans using patch test clinic populations.
The human maximization test (HMT) is a method to evaluate potency in humans, while the local lymph node assay (LLNA) is a test method that allows for the measuring of the allergic potency of a substance in a rodent. It has been proposed that an EC3 value (the value obtained by the LLNA test, ie, the concentration of an allergen leading to a 3-fold increase of baseline proliferation rate) would be a reliable indicator for a compound's allergic potency in humans. This paper compares the correlation between the EC3 value of a compound and its allergic occurrence in the general population with the correlation between the HMT of the compound and its allergic occurrence in the general population, to determine the relationship to potency. The correlation values when outliers were removed from the sample were -0.56 and -0.71 for LLNA and HMT, respectively, suggesting that there is a possible 20% error margin in LLNA's ability to predict potency. The data also suggest that other factors (such as exposure) could play up to a 30% role in the determination of allergic occurrence in the general population. The potency assays might be made more clinically relevant for predicting allergic frequencies by including a frequency factor and other factors in its dermatotoxicological interpretation.
Zaghi
Danny
D
Albert Einstein College of Medicine, NY, USA.
Maibach
Howard I
HI
D000485:Allergens / Q000633:toxicity*; D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects; D015510:Clinical Medicine; D017449:Dermatitis, Allergic Contact / Q000175:diagnosis / Q000209:etiology*; D006801:Humans; D023441:Local Lymph Node Assay; D008198:Lymph Nodes / Q000187:drug effects / Q000473:pathology; D051379:Mice; D010328:Patch Tests / Q000379:methods; D011237:Predictive Value of Tests; D012684:Sensory Thresholds; D012867:Skin / Q000187:drug effects / Q000473:pathology
D000485:Allergens