Simple spatial phase modulator for focal modulation microscopy.
Focal modulation microscopy (FMM) is an emerging microscopy technique for fluorescence imaging of thick biological tissue in vivo. A spatial phase modulator is a critical component whose characteristics have a significant impact on the performance of a FMM system. We have designed a simple spatial phase modulator based on a tilting glass plate that provides superb modulation stability. Image quality has been improved remarkably after integrating such a modulator into a FMM system.
Wong
Chee Howe
CH
Department of Electrical and Computer Engineering, National University of Singapore, Singapore.
Chong
Shau Poh
SP
Sheppard
Colin J R
CJ
Chen
Nanguang
N
D000465:Algorithms; D000818:Animals; D002478:Cells, Cultured; D019902:Chondrocytes; D008856:Microscopy, Fluorescence / Q000295:instrumentation* / Q000379:methods; D008858:Microscopy, Phase-Contrast / Q000295:instrumentation / Q000379:methods