Effects of anserine on the renal sympathetic nerve activity and blood pressure in urethane-anesthetized rats.
Previous studies have demonstrated that central injection of L-carnosine (beta-alynyl-L-histidine), dipeptide synthesized in mammalian muscles, affects renal sympathetic nerve activity (RSNA) and blood pressure (BP) in anesthetized rats. In the present study, using urethane-anesthetized rats, we examined the dose-dependent effects of intravenous (IV) injection of various doses of anserine, dipeptide of similar structure to L-carnosine, on RSNA, BP and heart rate (HR). We found that injection of a low dose of anserine (1 microg) significantly suppressed RSNA, BP and HR. Conversely, a high dose (1000 microg) of anserine significantly elevated RSNA, BP and HR. Pretreatment with lateral cerebral ventricular (LCV) injection of thioperamide, a histaminergic H(3)-receptor antagonist, eliminated the effects of a low dose of anserine on RSNA, BP and HR. LCV injection of diphenhydramine, a histaminergic H(1)-receptor antagonist, abolished the effects of a high dose of anserine on RSNA, BP and HR. These findings suggest that anserine affects RSNA, BP and HR in a dose-dependent manner, and that the histaminergic nerve may be involved in the dose-different effects of anserine in rats.
Tanida
M
M
College of Life Sciences, Ritsumeikan University, Kusatsu, Shiga, Japan. mtanida@sk.ritsumei.ac.jp
Shen
J
J
Kubomura
D
D
Nagai
K
K
D018686:Anesthetics, Intravenous; D000818:Animals; D000861:Anserine / Q000494:pharmacology*; D001794:Blood Pressure / Q000187:drug effects* / Q000502:physiology; D004151:Dipeptides / Q000494:pharmacology; D004155:Diphenhydramine / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D006321:Heart / Q000187:drug effects / Q000502:physiology; D006634:Histamine H1 Antagonists / Q000494:pharmacology; D054828:Histamine H3 Antagonists / Q000494:pharmacology; D007275:Injections, Intravenous; D007668:Kidney / Q000098:blood supply / Q000294:innervation / Q000502:physiology; D008297:Male; D010880:Piperidines / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D013564:Sympathetic Nervous System / Q000187:drug effects* / Q000502:physiology; D014520:Urethane
D018686:Anesthetics, Intravenous; D004151:Dipeptides; D006634:Histamine H1 Antagonists; D054828:Histamine H3 Antagonists; D010880:Piperidines; D014520:Urethane; D004155:Diphenhydramine; D000861:Anserine; C052075:thioperamide