Genetic contribution of chemokine receptor 2 (CCR2) polymorphisms towards increased serum total IgE levels in Indian asthmatics.
The chemokine (C-C motif) receptors (CCR) 2 and 5 are members of a large family of G protein-coupled receptors, playing important roles in asthma pathogenesis. Using standard sequencing techniques, a total of 15 single nucleotide and 8 insertion/deletion polymorphisms (DIPs) (5 novels) were identified in and around these two genes. None of the studied polymorphisms (N=7, selected on the basis on linkage disequilibrium) was associated with asthma in a case (N=315) - control (N=337) study and showed no evidence for non-random transmission to individuals with asthma/atopy in Indian pedigrees (n=235). However, multilocus haplotype analysis based on simulations yielded a P=0.00005 in the case-control study and a P=0.03 for the family-based association studies. Furthermore, rs3918356 and rs743660 polymorphisms in CCR2 were found to be associated with total serum IgE levels in both the study designs. Thus, our study supports a significant role for chemokine receptor polymorphisms in genetic susceptibility to asthma.
Batra
Jyotsna
J
Institute of Genomics and Integrative Biology, Delhi, India. jbatra9@hotmail.com
Ghosh
Balaram
B
D001249:Asthma / Q000097:blood* / Q000235:genetics* / Q000378:metabolism; D016022:Case-Control Studies; D008040:Genetic Linkage; D005819:Genetic Markers / Q000235:genetics; D020022:Genetic Predisposition to Disease; D006239:Haplotypes / Q000235:genetics*; D006801:Humans; D007073:Immunoglobulin E / Q000097:blood*; D007194:India; D015810:Linkage Disequilibrium; D011110:Polymorphism, Genetic / Q000235:genetics*; D054390:Receptors, CCR2 / Q000235:genetics*
C515334:CCR2 protein, human; D005819:Genetic Markers; D054390:Receptors, CCR2; D007073:Immunoglobulin E