title: Novel antimicrobial peptides isolated from skin secretions of the Mexican frog Hyla eximia.
abstract: In this work, we describe the original characterization of peptides and proteins present in the skin secretions of the Mexican amphibian Hyla eximia. To this purpose, a novel water/dark extraction method, as well as the classic electrical stimulation procedure, was applied in order to extract the skin secretion. Two novel antimicrobial peptides He-1 and He-2 were sequenced. In addition, a molecular mass fingerprint revealed more than one hundred different molecules. Eight peptides in homogeneous form were assayed against five species of bacteria. Thereafter, the peptide He-2 demonstrated high antiparasitic activity against ookinete forms of malaria parasites at low concentration.
authors.affiliation: Unidad de Proteómica, Instituto de Biotecnología-UNAM, Av. Universidad 2001, Col Chamilpa, CP 62210, Cuernavaca, Mor-México.
authors.forename: Oscar
authors.initials: O
authors.lastname: Villa-Hernández
authors.forename: Lorena
authors.initials: L
authors.lastname: Hernández-Orihuela
authors.forename: Maria
authors.initials: M
authors.lastname: del Carmen Rodríguez
authors.forename: Fernando
authors.initials: F
authors.lastname: Zamudio-Zuñiga
authors.forename: Ruben
authors.initials: R
authors.lastname: Castro-Franco
authors.forename: Victoria
authors.initials: V
authors.lastname: Pando
authors.forename: Cesar V F
authors.initials: CV
authors.lastname: Batista
mesh_terms: D000818:Animals; D023181:Antimicrobial Cationic Peptides / Q000302:isolation & purification* / Q000378:metabolism / Q000494:pharmacology; D001001:Anura; D001419:Bacteria; D012634:Bodily Secretions / Q000737:chemistry*; D049109:Cell Proliferation / Q000187:drug effects; D005591:Chemical Fractionation / Q000379:methods; D008800:Mexico; D008826:Microbial Sensitivity Tests; D010962:Plasmodium berghei; D020539:Sequence Analysis, Protein; D012867:Skin / Q000378:metabolism*
chemical_list: D023181:Antimicrobial Cationic Peptides