Differential modulation of beta-defensin gene expression by Salmonella Enteritidis in intestinal epithelial cells from resistant and susceptible chicken inbred lines.
beta-Defensins are important components of innate immunity in mucosal tissue, a major entry site for several pathogens. These small cationic peptides possess antimicrobial activity against various microorganisms including Salmonella. Two chicken inbred lines, 6 and 15I, diverge phenotypically with respect to levels of Salmonella Enteritidis intestinal carriage and to level of gene expression of two beta-defensins, AvBD1 and AvBD2. The cellular source of these two defensins in the intestinal tissue has not previously been explored. Therefore embryonic intestinal cells were isolated from both chicken lines. Primary intestinal cell cultures expressed epithelial specific markers (villin and E-cadherin) and differentially expressed two beta-defensin genes AvBD1 and AvBD2 according to chicken line. Furthermore, S. Enteritidis interfered with AvBD2 expression only in the cells from the susceptible line 15I. Our embryonic cell culture model demonstrated that intestinal epithelium express beta-defensin antimicrobial peptides that may play a role in immunoprotection against Salmonella Enteritidis.
Derache
Chrystelle
C
INRA, UR 1282 Infectiologie Animale et Santé Publique, Nouzilly, France.
Esnault
Evelyne
E
Bonsergent
Claire
C
Le Vern
Yves
Y
Quéré
Pascale
P
Lalmanach
Anne-Christine
AC
D000818:Animals; D002645:Chickens / Q000235:genetics / Q000276:immunology* / Q000382:microbiology; D005786:Gene Expression Regulation; D054884:Host-Pathogen Interactions / Q000276:immunology; D007113:Immunity, Innate; D018928:Immunity, Mucosal; D007413:Intestinal Mucosa / Q000276:immunology* / Q000382:microbiology; D007422:Intestines / Q000276:immunology / Q000382:microbiology; D011201:Poultry Diseases / Q000276:immunology* / Q000382:microbiology; D012481:Salmonella Infections, Animal / Q000276:immunology* / Q000382:microbiology; D012477:Salmonella enteritidis; D023083:beta-Defensins / Q000235:genetics*
D023083:beta-Defensins