A generally applicable assay for the quantification of inhibitory effects on PCR.
PCR amplification of target genes from environmental DNA extracts can suffer from PCR inhibition, caused by co-extracted substances. No simple assay has been available to quantify this inhibition. Therefore, a generally applicable PCR inhibition-assay was developed, which allows determination of statistically significant inhibition of PCR. This information is important when documenting quality of DNA in environmental extracts used as template for PCR.
Schneider
Salome
S
Molecular Ecology, Agroscope Reckenholz-Tänikon Research Station ART, Reckenholzstrasse 191, 8046 Zürich, Switzerland.
Enkerli
Jürg
J
Widmer
Franco
F
D004783:Environmental Microbiology; D004791:Enzyme Inhibitors / Q000032:analysis*; D019384:Nucleic Acid Synthesis Inhibitors; D016133:Polymerase Chain Reaction / Q000379:methods* / Q000592:standards*
D004791:Enzyme Inhibitors; D019384:Nucleic Acid Synthesis Inhibitors