A super-family of genes coding for secreted salivary gland proteins from the Hessian fly, Mayetiola destructor.
We have previously characterized a gene coding for the secreted-salivary-gland-protein 11A1 (SSGP-11A1) from the Hessian fly, Mayetiola destructor (Say) (Diptera Cecidomyiidae). Here we report the cloning and characterization of three new genes coding for proteins designated SSGP-11B1, SSGP-11C1, and SSGP-11C2, and their relationship with the SSGP-11A1-encoding gene. Based on their structural conservation, similar regulation, and clustered genomic organization, we conclude that the four genes represent a gene superfamily, designated SSGP-11, which originated from a common ancestor. Cloning, Southern blot and in situ hybridization data suggest that each of theSSGP-11 families has multiple members that cluster within short chromosome regions. The presence of a secretion signal peptide, the exclusive expression in the larval stage, and the clustered genomic organization indicate that this superfamily might be important for Hessian fly virulence/avirulence.
Chen
Ming-Shun
MS
USDA-ARS, Plant Science and Entomology Research Unit, Manhattan, KS 66506, USA. ming-shun.chen@gmprc.ksu.edu
Fellers
John P
JP
Zhu
Yu Cheng
YC
Stuart
Jeffrey J
JJ
Hulbert
Scot
S
El-Bouhssini
Mustapha
M
Liu
Xiang
X
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D002874:Chromosome Mapping; D017124:Conserved Sequence; D004175:Diptera / Q000235:genetics* / Q000378:metabolism; D017344:Genes, Insect / Q000235:genetics*; D008969:Molecular Sequence Data; D012471:Salivary Proteins and Peptides / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D016415:Sequence Alignment
D012471:Salivary Proteins and Peptides