Effects of acupuncture on expressions of the transcription factors NF-E2, YB-1, LRG47 in the SAMP10 mice.
OBJECTIVE
To explore the mechanism of acupuncture for delaying aging.


METHODS
Using the senescence accelerated mouse pattern SAMP10 and the normal aging mice SAMR1 as models and applying RT-PCR and digoxin (DIG)-labeled Northern blot technique to observe expressions of NF-E2, YB-1, LRG47 genes in the forebrain, cortex and hippocampus in a 8-month old SAMR1 control group, a 8-month old SAMP10 control group, a 8-month old SAMP10 acupuncture group and a 8-month old SAMP10 non-point stimulation group.


RESULTS
In the SAMP10 control group, the expressions of NF-E2, YB-1 and LRG47 were down-regulated in the forebrain, cortex and hippocampus, and after acupuncture they were up-regulated and tended to normal.


CONCLUSIONS
The brain aging of the SAMP10 mice is related with abnormal expressions of NF-E2, YB-1 and LRG47 genes; and acupuncture can regulate the expressions of NF-E2, YB-1 and LRG47 genes, strengthening the functions of erythrocyte series, increasing the proliferation of cells and enhancing the cellular immune function in anti-bacteria, hence delaying aging.
Fu
Yu
Y
The First Hospital Affiliated to Tianjin College of Traditional Chinese Medicine, Tianjin 300193, China.
Yu
Jian-chun
JC
Ding
Xiao-rong
XR
Han
Jingoxian
J
D015670:Acupuncture Therapy; D000375:Aging / Q000235:genetics* / Q000378:metabolism; D000818:Animals; D005260:Female; D019204:GTP-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D015870:Gene Expression; D006801:Humans; D008297:Male; D051379:Mice; D008822:Mice, Transgenic; D023421:Models, Animal; D050984:NF-E2 Transcription Factor, p45 Subunit / Q000235:genetics* / Q000378:metabolism; D011897:Random Allocation; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism
D019204:GTP-Binding Proteins; D050984:NF-E2 Transcription Factor, p45 Subunit; D014157:Transcription Factors; C096000:Ifi1 protein, mouse; C494693:Nfe2 protein, mouse; C541744:Ybx1 protein, mouse