Effect of human umbilical cord blood cells on Ang-II-induced hypertrophy in mice.
We have assessed the capacity of human umbilical cord blood (hUCB)-derived stem cells to differentiate into cardiomyocytes and repair angiotensin II induced insult in culture and in mouse hearts when injected. hUCB were able to differentiate into cardiomyocyte-like cells, when induced with 5-azacytidine or co-cultured with rat neonatal cardiomyocytes (NRCM). When co-cultured, hUCB reversed the pathological effects induced by angiotensin II (Ang-II) in NRCM and in mice injected after Ang-II infusion. As assessed by increased heart weight to body mass ratio and Ang-II-induced fibrosis, cardiac hypertrophy was also reduced after hUCB were injected. hUCB also reversed the pathological heart failure markers induced by Ang-II in mice. Further, we observed a shift from pathological hypertrophy towards physiological hypertrophy by hUCB in Ang-II-challenged mice. Our findings support hUCB as a feasible model for experimentation in stem cell therapy and emphasize the relevance of the hUCB in reversing heart failure conditions.
Vanamala
Sravan K
SK
Department of Cancer Biology & Pharmacology, University of Illinois, College of Medicine at Peoria, Peoria, IL 61656, USA.
Gopinath
Sreelatha
S
Gondi
Christopher S
CS
Rao
Jasti S
JS
D000804:Angiotensin II / Q000494:pharmacology; D000818:Animals; D017209:Apoptosis; D015415:Biomarkers / Q000378:metabolism; D006332:Cardiomegaly / Q000139:chemically induced / Q000473:pathology / Q000628:therapy*; D002454:Cell Differentiation; D018920:Coculture Techniques; D005312:Fetal Blood / Q000166:cytology* / Q000637:transplantation*; D015395:Histocompatibility Antigens Class I / Q000096:biosynthesis; D006801:Humans; D051379:Mice; D032383:Myocytes, Cardiac / Q000502:physiology / Q000637:transplantation*; D051381:Rats; D033581:Stem Cell Transplantation; D013234:Stem Cells / Q000502:physiology*
D015415:Biomarkers; D015395:Histocompatibility Antigens Class I; D000804:Angiotensin II