Protective effect of an extract from Periplaneta americana on hematopoiesis in irradiated rats.
OBJECTIVE
To investigate the protective effect of W(11)-a(12), an extract from Periplaneta americana, on hematopoiesis in irradiated rats.


METHODS
Wistar rats receiving total body irradiation of (60)Co gamma-rays alone or with combined radiation and skin wound injury were used in this study. W(11)-a(12) was applied either topically into the skin wounds or systemically by intraperitoneal injection. The numbers of white blood cells in peripheral blood, the nucleated cells and the colony-forming unit of granulocyte/macrophage progenitors (CFU-GM) in bone marrow were measured, respectively.


RESULTS
Topical application of W(11)-a(12) into skin wounds in rats with combined 6 Gy total body irradiation and skin wound injury could increase the neutrophils and macrophages in the wounded area and the nucleated cells in bone marrow at 24 h and 48 h, while the peripheral white blood cells did not show significant change. However, in rats with 4 Gy total body irradiation alone, the peripheral white blood cells, bone marrow nucleated cells and the number of colony-forming unit of granulocyte-macrophage progenitors were all significantly higher in the treatment groups by intraperitoneal injection of W(11)-a(12) than those in the control groups by injection of normal saline at days 3 and days 5 after radiation.


CONCLUSIONS
W(11)-a(12) showed a protective effect on hematopoiesis after total body irradiation and could increase the inflammatory cells in wounded tissues at the initiation stage after irradiation, which will benefit the management of combined radiation and skin wound injury.
Chen
Xiao-Hong
XH
State Key Laboratory of Trauma, Burns and Combined Injury, Institute of Combined Injury of PLA, Chongqing, China.
Ran
Xin-Ze
XZ
Sun
Ren-Shan
RS
Shi
Chun-Meng
CM
Su
Yongping
Y
Guo
Chao-Hua
CH
Cheng
Tian-Min
TM
D000818:Animals; D006410:Hematopoiesis / Q000187:drug effects / Q000528:radiation effects*; D020556:Neutrophil Infiltration / Q000528:radiation effects; D010526:Periplaneta; D010936:Plant Extracts / Q000494:pharmacology*; D011837:Radiation-Protective Agents / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D014916:Whole-Body Irradiation; D014945:Wound Healing / Q000187:drug effects
D010936:Plant Extracts; D011837:Radiation-Protective Agents