title: GT1-7 cell-based cytoxicity screening assay on 96-well microplates as a platform for the safety assessment of genetically modified Gerbera hybrida extracts.
abstract: In this investigation, a GT1-7 cell-based cytotoxicity screening assay in 96-well microplates was set up. The assay, using propidium iodide fluorescence, was proven to be reliable, with good quality (Z' = 0.51) and low plate-to-plate and day-to-day variations. Further on, a library containing extracts from 227 genetic modification (GM) Gerbera hybrida and 42 Gerbera varieties was screened; however, no differences between them were found. Based on these findings, we propose the use of the current assay within the first-tier screening studies of large collections. Also, these results provide valuable information for GM Gerbera risk-assessment purposes and offer a model for the toxicity cell-based screening of GM crops.
authors.affiliation: Department of Biochemistry and Pharmacy, Faculty of Mathematics and Natural Sciences, Abo Akademi University, Abo, Finland.
authors.forename: Adyary
authors.initials: A
authors.lastname: Fallarero
authors.forename: Miia
authors.initials: M
authors.lastname: Ainasoja
authors.forename: Malena
authors.initials: M
authors.lastname: Sandberg
authors.forename: Teemu H
authors.initials: TH
authors.lastname: Teeri
authors.forename: Pia M
authors.initials: PM
authors.lastname: Vuorela
mesh_terms: D019659:Asteraceae / Q000166:cytology* / Q000235:genetics; D002478:Cells, Cultured; D003257:Consumer Product Safety / Q000331:legislation & jurisprudence*; D018556:Crops, Agricultural; D004304:Dosage Forms; D004353:Drug Evaluation, Preclinical; D018506:Gene Expression Regulation, Plant; D005818:Genetic Engineering / Q000379:methods*; D030821:Plants, Genetically Modified / Q000166:cytology* / Q000235:genetics; D018570:Risk Assessment / Q000379:methods
chemical_list: D004304:Dosage Forms