title: Regulation and action of fibroblast growth factor 17 in bovine follicles.
abstract: Fibroblast growth factor 17 (FGF17) is a member of the FGF8 subfamily that appears to be relevant to folliculogenesis and oogenesis, as the prototype member FGF8 is an oocyte-derived protein that signals to cumulus cells. FGF8 has structural and receptor-binding similarities to FGF17, whose expression in the ovary has not been reported. In this study, we demonstrate localization of FGF17 protein to the oocyte of preantral follicles, and to the oocyte and granulosa cells of antral follicles. Real-time PCR demonstrated the presence of mRNA in oocytes and, to a lesser extent, in granulosa and theca cells. FGF17 mRNA abundance was low in granulosa and theca cells from healthy follicles and increased significantly in atretic follicles. Addition of FSH or IGF-I to granulosa cells in vitro decreased FGF17 mRNA abundance, and treatment with FGF17 inhibited estradiol and progesterone secretion from granulosa cells in relation to control cultures without these additives. We conclude that FGF17 is a potential mediator of granulosa cell differentiation.
authors.affiliation: Departamento de Reprodução Animal, Faculdade de Medicina Veterinária e Zootecnia, Universidade Estadual Paulista, Botucatu, São Paulo 18618-000, Brazil.
authors.forename: M F
authors.initials: MF
authors.lastname: Machado
authors.forename: V M
authors.initials: VM
authors.lastname: Portela
authors.forename: C A
authors.initials: CA
authors.lastname: Price
authors.forename: I B
authors.initials: IB
authors.lastname: Costa
authors.forename: P
authors.initials: P
authors.lastname: Ripamonte
authors.forename: R L
authors.initials: RL
authors.lastname: Amorim
authors.forename: J
authors.initials: J
authors.lastname: Buratini
mesh_terms: D000818:Animals; D002417:Cattle; D002454:Cell Differentiation / Q000187:drug effects / Q000502:physiology; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D004958:Estradiol / Q000378:metabolism; D005260:Female; D005346:Fibroblast Growth Factors / Q000235:genetics* / Q000378:metabolism* / Q000494:pharmacology; D005786:Gene Expression Regulation / Q000502:physiology; D006107:Granulosa Cells / Q000166:cytology / Q000187:drug effects / Q000502:physiology; D007334:Insulin-Like Growth Factor I / Q000494:pharmacology; D009865:Oocytes / Q000166:cytology / Q000187:drug effects / Q000502:physiology; D009866:Oogenesis / Q000502:physiology*; D006080:Ovarian Follicle / Q000166:cytology* / Q000502:physiology*; D011374:Progesterone / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D013799:Theca Cells / Q000166:cytology / Q000187:drug effects / Q000502:physiology
chemical_list: C484724:FGF17 protein, human; D012333:RNA, Messenger; D011374:Progesterone; D004958:Estradiol; D005346:Fibroblast Growth Factors; D007334:Insulin-Like Growth Factor I