title: Pharmacological analysis of the neutrophil migration induced by D. rostrata lectin: involvement of cytokines and nitric oxide.
abstract: In the present study, we investigated the involvement of resident cell and inflammatory mediators in the neutrophil migration induced by chemotactic activity of a glucose/mannose-specific lectin isolated from Dioclea rostrata seeds (DrosL). Rats were injected i.p. with DrosL (125-1000 microg/cavity), and at 2-96 h thereafter the leukocyte counts in peritoneal fluid were determined. DrosL-induced a dose-dependent neutrophil migration accumulation, which reached maximal response at 24 h after injection and declines thereafter. The carbohydrate ligand nearly abolished the neutrophil influx. Pre-treatment of peritoneal cavities with thioglycolate which increases peritoneal macrophage numbers, enhanced neutrophil migration induced by DrosL by 303%. However, the reduction of peritoneal mast cell numbers by treatment of the cavities with compound 48/80 did not modify DrosL-induced neutrophil migration. The injection into peritoneal cavities of supernatants from macrophage cultures stimulated with DrosL (125, 250 and 500 microg/ml) induced neutrophil migration. In addition, DrosL treatment induced cytokines (TNF-alpha, IL-1beta and CINC-1) and NO release into the peritoneal cavity of rats. Finally, neutrophil chemotaxis assay in vitro showed that the lectin (15 and 31 microg/ml) induced neutrophil chemotaxis by even 180%. In conclusion, neutrophil migration induced by D. rostrata lectin occurs by way of the release of NO and cytokines such as IL-1beta, TNF-alpha and CINC-1.
authors.affiliation: Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Ceará, Caixa Postal 60451-970, Fortaleza, CE, Brazil. jozigodoy@gmail.com
authors.forename: Jozi G
authors.initials: JG
authors.lastname: Figueiredo
authors.forename: Flávio S
authors.initials: FS
authors.lastname: Bitencourt
authors.forename: Mário R L
authors.initials: MR
authors.lastname: Mota
authors.forename: Priscila P
authors.initials: PP
authors.lastname: Silvestre
authors.forename: Cibele N
authors.initials: CN
authors.lastname: Aguiar
authors.forename: Raquel G
authors.initials: RG
authors.lastname: Benevides
authors.forename: Kyria S
authors.initials: KS
authors.lastname: Nascimento
authors.forename: Tales R
authors.initials: TR
authors.lastname: de Moura
authors.forename: Daniela
authors.initials: D
authors.lastname: Dal-Secco
authors.forename: Ana M S
authors.initials: AM
authors.lastname: Assreuy
authors.forename: Fernando de Q
authors.initials: Fde Q
authors.lastname: Cunha
authors.forename: Marcus R
authors.initials: MR
authors.lastname: Vale
authors.forename: Benildo S
authors.initials: BS
authors.lastname: Cavada
authors.forename: Nylane M N
authors.initials: NM
authors.lastname: Alencar
mesh_terms: D000818:Animals; D002465:Cell Movement / Q000187:drug effects*; D016207:Cytokines / Q000502:physiology*; D052598:Dioclea / Q000737:chemistry*; D037102:Lectins / Q000494:pharmacology*; D008297:Male; D009504:Neutrophils / Q000166:cytology / Q000187:drug effects*; D009569:Nitric Oxide / Q000502:physiology*; D010936:Plant Extracts / Q000737:chemistry*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D016207:Cytokines; D037102:Lectins; D010936:Plant Extracts; D009569:Nitric Oxide