Fingerprinting analysis of Rhizoma chuanxiong of commercial types using 1H nuclear magnetic resonance spectroscopy and high performance liquid chromatography method.
The (1)H nuclear magnetic resonance ((1)H NMR) fingerprints of fractionated non-polar extracts (control substance for a plant drug (CSPD) A) from Rhizoma chuanxiong, the rhizomes of Ligusticum chuanxiong Hort., of seven specimens from different sources were measured on Fourier Transform (FT)-NMR spectrometer and assigned by comparing them with the (1)H NMR spectra of the isolated pure compounds. The (1)H NMR fingerprints showed exclusively characteristic resonance signals of the major special constituents of the plant. Although the differences in the relative intensity of the (1)H NMR signals due to a discrepancy in the ratio of the major constituents among these samples could be confirmed by high performance liquid chromatography analysis, the general features of the (1)H NMR fingerprint established for an authentic sample of the rhizomes of L. chuanxiong exhibited exclusive data from those special compounds and can be used for authenticating L. Chuanxiong species.
Qin
Hai-Lin
HL
Institute of Materia Medica, Chinese Academy of Medical Sciences, Peking Union Medical College, Key Laboratory of Bioactive Substances and Resources of Chinese Herbal Medicine, Ministry of Education, Beijing, China. qinhailin@imm.ac.cn
Deng
An-Jun
AJ
Du
Guan-Hua
GH
Wang
Peng
P
Zhang
Jin-Lan
JL
Li
Zhi-Hong
ZH
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000032:analysis* / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*
D004365:Drugs, Chinese Herbal; C534193:chuanxiong rhizome preparation