Role of protein kinase C in advanced glycation end products-induced epithelial-mesenchymal transition in renal proximal tubular epithelial cells.
The role of protein kinase C (PKC) activation in advanced glycation end products (AGEs)-induced epithelial-mesenchymal transition in renal proximal tubular epithelial cells was investigated. HKC cells were divided into three groups: normal group, AGE-BSA group (100 mg/L AGE-BSA) and AGE-BSA+PKC inhibitor (10 mumol/L chelerythrine chloride) group. PKC activity was measured by PKC assay kit. The expression of Vimentin, and phosphorylated beta-catenin was detected by using Western blotting, and the content of TGF-beta1 was examined by ELISA method. The intracellular disposition of Vimentin was observed by fluorescence microscopy. As compared with normal group, PKC activity was increased significantly in AGE-BSA group. The expression of Vimentin, phosphorylated beta-catenin, and TGF-beta1 was enhanced significantly in AGE-BSA group. The expression of Vimentin, phosphorylated beta-catenin, and TGF-beta1 was significantly blocked by chelerythrine chloride. High expression of Vimentin, phosphorylated beta-catenin, and TGF-beta1 induced by AGE-BSA may be mediated via the activation of PKC signal transduction pathway.
Ge
Shuwang
S
Department of Nephrology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China. geshuwang@yahoo.com.cn
Zeng
Rui
R
Luo
Yun
Y
Liu
Lin
L
Wei
Honglan
H
Zhang
Juan
J
Zhou
Huan
H
Xu
Gang
G
D002460:Cell Line; D054338:Cell Transdifferentiation / Q000187:drug effects; D004847:Epithelial Cells / Q000166:cytology* / Q000378:metabolism; D017127:Glycation End Products, Advanced / Q000494:pharmacology*; D006801:Humans; D007687:Kidney Tubules, Proximal / Q000166:cytology* / Q000187:drug effects; D008648:Mesoderm / Q000166:cytology*; D011493:Protein Kinase C / Q000378:metabolism*; D053773:Transforming Growth Factor beta1 / Q000378:metabolism; D014746:Vimentin / Q000378:metabolism; D051176:beta Catenin / Q000378:metabolism
D017127:Glycation End Products, Advanced; D053773:Transforming Growth Factor beta1; D014746:Vimentin; D051176:beta Catenin; D011493:Protein Kinase C