CpG oligonucleotides activate the immune response in burned mice.
BACKGROUND
Immunosuppression after burn injury increases the risk of sepsis and multiple organ failure. We examined changes of immune function in mice after burn injury and investigated the immunostimulatory effect of oligodeoxynucleotides containing CpG motifs.


METHODS
Male BALB/c mice (8-10 wk old) received a full-thickness burn to 20% of their body surface area, after which the immunological parameters of splenic macrophages were evaluated. To assess the immunostimulatory effect of oligodeoxynucleotide treatment, splenic macrophages harvested from burned mice were incubated with oligodeoxynucleotides. Then cytokine production and major histocompatibility complex class II antigen expression were measured. To assess the in vivo effect of oligodeoxynucleotides, intraperitoneal administration was done on day 4 after burn injury, and class II antigen expression by splenic macrophages was measured 10 d later.


RESULTS
Class II antigen expression and the synthesis of cytokines (interleukin-12, tumor necrosis factor-alpha, interleukin-6, and interleukin-1) by splenic macrophages were significantly reduced after burn injury, while incubation of splenic macrophages from burned mice with oligodeoxynucleotides partially enhanced the production of interleukin-12, tumor necrosis factor-alpha, interleukin-6, and interleukin-1. In addition, intraperitoneal administration of oligodeoxynucleotides enhanced class II antigen expression by splenic macrophages.


CONCLUSIONS
The reduction of class II antigen expression and synthesis of cytokines (interleukin-12, tumor necrosis factor-alpha, interleukin-6, and interleukin-1) by splenic macrophages after burn injury was partially reversed by oligodeoxynucleotide treatment. Therefore, immunostimulatory oligodeoxynucleotides may be a potential treatment for post-burn immunosuppression.
Yabuki
Teru
T
Department of Emergency and Critical Care Medicine, Kansai Medical University, Moriguchi, Osaka, Japan. yabuki@aichi-med-u.ac.jp
Takeyama
Naoshi
N
Tsuda
Masanobu
M
Saitoh
Fukuki
F
Tanaka
Takaya
T
Noguchi
Hiroshi
H
Nakatani
Toshio
T
D000818:Animals; D002056:Burns / Q000188:drug therapy* / Q000276:immunology; D018899:CpG Islands / Q000276:immunology*; D016207:Cytokines / Q000378:metabolism; D015536:Down-Regulation; D015870:Gene Expression; D005802:Genes, MHC Class II; D008264:Macrophages / Q000378:metabolism; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009838:Oligodeoxyribonucleotides / Q000276:immunology / Q000627:therapeutic use*; D013154:Spleen / Q000276:immunology
D016207:Cytokines; D009838:Oligodeoxyribonucleotides