Fluorescence logic-signal-based multiplex detection of nucleases with the assembly of a cationic conjugated polymer and branched DNA.
An energy-transfer cascade is generated from a cationic conjugated polymer (PFP) and negatively charged, Y-shaped DNA labeled with three dyes at its termini (fluorescein (Fl), Tex Red, and Cy5). Multistep fluorescence resonance energy transfer regulates the fluorescence intensities of PFP and the dyes. Different types of logic gates can be operated by observing the emission wavelengths of different dyes with multiplex nucleases as inputs.
Feng
Xuli
X
Beijing National Laboratory for Molecular Sciences, Key Laboratory of Organic Solids, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190 PR China.
Duan
Xinrui
X
Liu
Libing
L
Feng
Fude
F
Wang
Shu
S
Li
Yuliang
Y
Zhu
Daoben
D
D015374:Biosensing Techniques / Q000379:methods*; D002412:Cations / Q000737:chemistry*; D004247:DNA / Q000737:chemistry*; D004262:DNA Restriction Enzymes / Q000032:analysis*; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods*; D005456:Fluorescent Dyes; D009690:Nucleic Acid Conformation; D011108:Polymers / Q000737:chemistry*
D002412:Cations; D005456:Fluorescent Dyes; D011108:Polymers; D004247:DNA; D004262:DNA Restriction Enzymes