title: Characterization of intracellular superoxide dismutase alterations in premalignant and malignant lesions of the oral cavity: correlation with lymph node metastasis.
abstract: OBJECTIVE
  The purpose of this study was to characterize changes in the expression of copper-zinc superoxide dismutase (Cu/Zn-SOD) and manganese SOD (Mn-SOD) in oral squamous-cell carcinoma (OSCC).
  
  
  METHODS
  Real-time quantitative reverse transcriptase-polymerase chain reaction analysis of Cu/Zn-SOD and Mn-SOD mRNA expression was carried out in 50 pairs of OSCC tissue specimens and corresponding normal tissues. Mn-SOD protein expression was evaluated further in 65 OSCC tissue samples and 33 oral premalignant lesions (OPLs) using immunohistochemistry.
  
  
  RESULTS
  Significant (P < 0.001) upregulation of Mn-SOD mRNA expression was observed in OSCC tissues compared with the normal tissue counterparts, whereas no significant difference was detected in Cu/Zn-SOD expression. Significant increases in Mn-SOD protein expression were seen in both OPLs (P < 0.001) and OSCC tissue (P < 0.001) together with a high incidence of lymph node metastasis (P = 0.04).
  
  
  CONCLUSIONS
  Our findings suggested that Mn-SOD overexpression is a frequent and early event during oral carcinogenesis and could contribute to aggressive OSCC.
authors.affiliation: Division of Dentistry and Oral-Maxillofacial Surgery, Chiba University Hospital, 1-8-1 Inohana, Chuo-ku, Chiba 260-8670, Japan.
authors.forename: Hidetaka
authors.initials: H
authors.lastname: Yokoe
authors.forename: Hitomi
authors.initials: H
authors.lastname: Nomura
authors.forename: Yukio
authors.initials: Y
authors.lastname: Yamano
authors.forename: Kazuaki
authors.initials: K
authors.lastname: Fushimi
authors.forename: Yosuke
authors.initials: Y
authors.lastname: Sakamoto
authors.forename: Katsunori
authors.initials: K
authors.lastname: Ogawara
authors.forename: Masashi
authors.initials: M
authors.lastname: Shiiba
authors.forename: Hiroki
authors.initials: H
authors.lastname: Bukawa
authors.forename: Katsuhiro
authors.initials: K
authors.lastname: Uzawa
authors.forename: Yuichi
authors.initials: Y
authors.lastname: Takiguchi
authors.forename: Hideki
authors.initials: H
authors.lastname: Tanzawa
mesh_terms: D000328:Adult; D000368:Aged; D002294:Carcinoma, Squamous Cell / Q000201:enzymology* / Q000473:pathology; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008207:Lymphatic Metastasis; D008297:Male; D008875:Middle Aged; D009062:Mouth Neoplasms / Q000201:enzymology* / Q000473:pathology; D011230:Precancerous Conditions / Q000201:enzymology*; D012333:RNA, Messenger / Q000032:analysis; D013482:Superoxide Dismutase / Q000032:analysis / Q000235:genetics / Q000502:physiology*
chemical_list: D012333:RNA, Messenger; D013482:Superoxide Dismutase