Effect of serum from mice immunized with group A streptococcus antigens on the efficiency of in vitro cloning of stromal precursor cells.
The efficiency of cloning of stromal precursor cell in mouse bone marrow culture increases significantly (2-3-fold) in the presence of serum from mice immunized with type 5 group A streptococcus antigens (5-20 microl serum/ml culture medium) in comparison with intact animal serum. The levels of TNF-alpha and IFN-gamma are significantly reduced (2.7 times and more than 6-fold, respectively) in the sera of immunized mice in comparison with normal serum. Serum levels of IL-2, -4, -5, -10, and -12 were about the same in both groups; no granulocyte-macrophage CSF was detected. These data attest to appreciable effect of immunization with streptococcal antigens on the bone marrow stromal tissue; this effect is presumably mediated through serum cytokines.
Gorskaya
U F
UF
Laboratory of Immunity Regulation, N. F. Gamaleya Institute of Epidemiology and Microbiology, the Russian Academy of Medical Sciences, Moscow, Russia. uliya.gorskaya@nearmedic.ru
Danilova
T A
TA
Lunin
V G
VG
Grabko
V I
VI
Sharapova
N E
NE
Nesterenko
V G
VG
D000818:Animals; D000942:Antigens, Bacterial / Q000276:immunology*; D001854:Bone Marrow Cells / Q000166:cytology*; D002478:Cells, Cultured; D002999:Clone Cells; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000378:metabolism; D006168:Guinea Pigs; D007371:Interferon-gamma / Q000378:metabolism; D016753:Interleukin-10 / Q000378:metabolism; D018664:Interleukin-12 / Q000378:metabolism; D007376:Interleukin-2 / Q000378:metabolism; D015847:Interleukin-4 / Q000378:metabolism; D015848:Interleukin-5 / Q000378:metabolism; D051379:Mice; D008808:Mice, Inbred CBA; D044967:Serum / Q000276:immunology* / Q000378:metabolism*; D013297:Streptococcus pyogenes / Q000276:immunology*; D017154:Stromal Cells / Q000166:cytology*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
D000942:Antigens, Bacterial; D007376:Interleukin-2; D015848:Interleukin-5; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10; D018664:Interleukin-12; D015847:Interleukin-4; D007371:Interferon-gamma; D016178:Granulocyte-Macrophage Colony-Stimulating Factor