[Construction of standard allelic ladder of miniSTR loci by molecular cloning].
OBJECTIVE
To investigate the genetic polymorphism of five miniSTR loci (D9S2157, D9S1122, D10S1435, D12ATA63, D2S1776) in Hebei Han population and to construct standard allelic ladders.


METHODS
Polymorphism of the five miniSTR loci in 120 unrelated individuals was analyzed by fluorescence PCR and ABI 310 Genetic Analyzer. Molecular cloning technique was employed to construct standard allelic ladder of the 5 loci.


RESULTS
Of the five miniSTR loci, 8, 8, 7, 5 and 8 alleles were found, respectively. The polymorphism information component were 0.790, 0.720, 0.750, 0.630 and 0.850, respectively.


CONCLUSIONS
The five loci have relatively abundant polymorphic information and their standard allelic ladders constructed by molecular cloning technique are useful in forensic science.
Bai
Xue
X
Hebei Key Laboratory of Forensic Medicine, Department of Forensic Medicine, Hebei Medical University, Shijiazhuang 050017, China. snowhome2004@126.com
Cong
Bin
B
Li
Shu-Jin
SJ
Hou
Zhi-Ping
ZP
Gu
Jian-Li
JL
Liu
Ning
N
Li
Xia
X
Guo
Xia
X
D000483:Alleles; D044466:Asian People / Q000235:genetics; D002681:China / Q000208:ethnology; D003001:Cloning, Molecular; D053803:Forensic Genetics; D005787:Gene Frequency; D005828:Genetics, Population; D006801:Humans; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic / Q000235:genetics*; D020080:Tandem Repeat Sequences / Q000235:genetics*