Using a microfluidic device for high-content analysis of cell signaling.
Quantitative analysis and understanding of signaling networks require measurements of the location and activities of key proteins over time, at the level of single cells, in response to various perturbations. Microfluidic devices enable such analyses to be conducted in a high-throughput and in a highly controlled manner. We describe in detail how to design and use a microfluidic device to perform such information-rich experiments.
Cheong
Raymond
R
Department of Biomedical Engineering and Whitaker Institute of Biomedical Engineering and Institute for Cell Engineering, The Johns Hopkins University, Baltimore, MD 21218, USA.
Wang
Chiaochun Joanne
CJ
Levchenko
Andre
A
D000818:Animals; D006801:Humans; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation* / Q000379:methods*; D015398:Signal Transduction