Murine Jagged1/Notch signaling in the second heart field orchestrates Fgf8 expression and tissue-tissue interactions during outflow tract development.
Notch signaling is vital for proper cardiovascular development and function in both humans and animal models. Indeed, mutations in either JAGGED or NOTCH cause congenital heart disease in humans and NOTCH mutations are associated with adult valvular disease. Notch typically functions to mediate developmental interactions between adjacent tissues. Here we show that either absence of the Notch ligand Jagged1 or inhibition of Notch signaling in second heart field tissues results in murine aortic arch artery and cardiac anomalies. In mid-gestation, these mutants displayed decreased Fgf8 and Bmp4 expression. Notch inhibition within the second heart field affected the development of neighboring tissues. For example, faulty migration of cardiac neural crest cells and defective endothelial-mesenchymal transition within the outflow tract endocardial cushions were observed. Furthermore, exogenous Fgf8 was sufficient to rescue the defect in endothelial-mesenchymal transition in explant assays of endocardial cushions following Notch inhibition within second heart field derivatives. These data support a model that relates second heart field, neural crest, and endocardial cushion development and suggests that perturbed Notch-Jagged signaling within second heart field progenitors accounts for some forms of congenital and adult cardiac disease.
High
Frances A
FA
Department of Cell and Developmental Biology, Cardiovascular Institute, and Institute for Regenerative Medicine, University of Pennsylvania, Philadelphia, PA, USA.
Jain
Rajan
R
Stoller
Jason Z
JZ
Antonucci
Nicole B
NB
Lu
Min Min
MM
Loomes
Kathleen M
KM
Kaestner
Klaus H
KH
Pear
Warren S
WS
Epstein
Jonathan A
JA
D000818:Animals; D055415:Bone Morphogenetic Protein 4 / Q000235:genetics; D002135:Calcium-Binding Proteins / Q000502:physiology*; D002465:Cell Movement; D005260:Female; D051524:Fibroblast Growth Factor 8 / Q000235:genetics / Q000502:physiology*; D018507:Gene Expression Regulation, Developmental; D006321:Heart / Q000196:embryology*; D006330:Heart Defects, Congenital / Q000209:etiology; D019426:Integrases / Q000502:physiology; D036341:Intercellular Signaling Peptides and Proteins / Q000502:physiology*; D000072100:Jagged-1 Protein; D008565:Membrane Proteins / Q000502:physiology*; D051379:Mice; D009432:Neural Crest / Q000166:cytology; D051880:Receptors, Notch / Q000502:physiology*; D000072099:Serrate-Jagged Proteins; D015398:Signal Transduction / Q000502:physiology*
C525810:Bmp4 protein, mouse; D055415:Bone Morphogenetic Protein 4; D002135:Calcium-Binding Proteins; C496457:Fgf8 protein, mouse; D036341:Intercellular Signaling Peptides and Proteins; C000606273:JAG1 protein, human; C000606274:Jag1 protein, mouse; D000072100:Jagged-1 Protein; D008565:Membrane Proteins; D051880:Receptors, Notch; D000072099:Serrate-Jagged Proteins; D051524:Fibroblast Growth Factor 8; C045073:Cre recombinase; D019426:Integrases