Human formyl peptide receptor 1 (FPR1) c.32C>T SNP is associated with decreased soluble E-selectin levels.
OBJECTIVE
The human formyl peptide receptor (FPR) is a G protein-coupled chemoattractant receptor that is thought to mediate inflammatory responses. The FPR1 gene is highly polymorphic. In a recent study, the FPR1 c.32C>T SNP, resulting in the amino-acid substitution I11T, was reported to be significantly associated with C-reactive protein levels. Therefore, this study sought to determine if the impact of such a genetic variation extends to other clinical parameters associated with inflammation, including cytokines, adhesion molecules and inflammatory markers.


METHODS
This study was carried out on a subsample of 325 adults selected from the STANISLAS cohort study. The FPR1 c.32C>T SNP was genotyped using PCR amplification followed by restriction enzyme digestion. Anthropometric measurements and biochemical profiles were assessed for each individual.


RESULTS
The allele frequencies of FPR1 c.32C>T were 0.74 for the 32C allele and 0.26 for the 32T allele. Genotype frequencies were 0.55 for C/C, 0.38 for C/T and 0.07 for T/T. After adjusting for age, sex, BMI, alcohol and cigarette consumption, oral contraceptive, antibiotics and anti-inflammatory drug use, statistical analysis (under a recessive model of inheritance) demonstrated that serum E-selectin levels were 68% lower in individuals homozygous for T/T than in those with C/T or C/C genotypes (p = 0.001). However, no significant correlations were found for C-reactive protein or the other 18 tested clinical parameters that were analyzed in this study.


CONCLUSIONS
The FPR1 c.32C>T SNP may be associated with E-selectin levels in the French population. Although of importance, these findings need confirmation in larger studies.
Benachour
Hamanou
H
Nancy-Université, Nancy, France.
Zaiou
Mohamed
M
Herbeth
Bernard
B
Lambert
Daniel
D
Lamont
John V
JV
Pfister
Michèle
M
Siest
Gérard
G
Tiret
Laurence
L
Blankenberg
Stefan
S
Fitzgerald
Peter S
PS
Visvikis-Siest
Sophie
S
D000328:Adult; D000483:Alleles; D000886:Anthropometry; D015415:Biomarkers / Q000097:blood; D015331:Cohort Studies; D019040:E-Selectin / Q000097:blood*; D005260:Female; D005602:France; D005787:Gene Frequency; D005808:Genes, Recessive; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006720:Homozygote; D006801:Humans; D007249:Inflammation / Q000209:etiology; D008297:Male; D008875:Middle Aged; D008957:Models, Genetic; D020641:Polymorphism, Single Nucleotide; D044042:Receptors, Formyl Peptide / Q000235:genetics*; D012995:Solubility
D015415:Biomarkers; D019040:E-Selectin; C469986:FPR1 protein, human; D044042:Receptors, Formyl Peptide