title: Genetic copy number alterations and IL-13 expression differences in papillary thyroid cancers and benign nodules.
abstract: Thyroid nodules were the extremely common endocrine tumors, in which papillary thyroid carcinomas (PTCs) were the most prevalent endocrine malignancy, representing 80-90% of all thyroid malignancies. It was still a dilemma to discriminate PTCs and benign thyroid nodules. With a new molecular genetics technology of Multiplex ligation-dependent probe amplification (MLPA), we investigated 13 PTC and 14 benign nodule tissue samples. The results showed that PTCs had more genetic copy number alteration than benign nodules (P < 0.001). Receiver operating characteristic (ROC) curve analysis suggested that genomic aberrations would provide a moderate accuracy method to discriminate PTCs and benign nodules. The gain of interleukin 13 (IL-13) gene obviously identified the great difference between PTCs and benign nodules. Immunohistochemistry also confirmed significantly higher IL-13 expression in the PTCs (P < 0.001). The current study showed that MLPA should be an effective method to diagnose PTCs and benign thyroid nodules, and also provided a clue to another relationship between IL-13 and PTCs.
authors.affiliation: Shanghai Institute of Endocrinology and Metabolism, Shanghai JiaoTong University School of Medicine, Shanghai, 200025, People's Republic of China.
authors.forename: ZeFei
authors.initials: Z
authors.lastname: Zhao
authors.forename: Qing
authors.initials: Q
authors.lastname: Wei
authors.forename: Yongju
authors.initials: Y
authors.lastname: Zhao
authors.forename: Fukang
authors.initials: F
authors.lastname: Sun
authors.forename: Xiaolong
authors.initials: X
authors.lastname: Jin
authors.forename: Bin
authors.initials: B
authors.lastname: Cui
authors.forename: Guang
authors.initials: G
authors.lastname: Ning
mesh_terms: D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000235:genetics*; D002291:Carcinoma, Papillary / Q000235:genetics* / Q000473:pathology; D003937:Diagnosis, Differential; D005260:Female; D018628:Gene Dosage; D005820:Genetic Testing / Q000379:methods; D006801:Humans; D007150:Immunohistochemistry; D018793:Interleukin-13 / Q000235:genetics* / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D009369:Neoplasms / Q000235:genetics* / Q000473:pathology; D013961:Thyroid Gland / Q000378:metabolism / Q000473:pathology; D013964:Thyroid Neoplasms / Q000235:genetics* / Q000473:pathology; D016606:Thyroid Nodule / Q000235:genetics* / Q000473:pathology
chemical_list: D014408:Biomarkers, Tumor; D018793:Interleukin-13