beta-1,4-Galactosyltransferase-I participates in lipopolysaccharide induced reactive microgliosis.
beta-1,4-Galactosyltransferase-I (beta-1,4-GalT-I) is one of the best studied glycosyltransferases. Previous studies demonstrated that beta-1,4-GalT-I was a major galactosyltransferase responsible for selectin-ligand biosynthesis and that inflammatory responses of beta-1,4-GalT-I deficient mice were impaired. Our previous study suggest that beta-1,4-GalT-I may play an important role in regulating immune cell migration into the inflammatory site. In this study, we investigate beta-1,4-GalT-I may play an important role in mediating microgliosis. The results of this study demonstrated that beta-1,4-GalT-I was strongly induced in the ventral midbrain by intranigral injection of LPS. Most galactose-containing glycans and beta-1,4-GalT-I were expressed in microglia. Moreover, an Ab against beta-1,4-GalT-I attenuated both LPS-induced microglial activation and phagocytosis. We therefore suggest that beta-1,4-GalT-I may play an important role in regulating immune cell migration into the inflammatory site and mediating microgliosis.
Chen
Jianping
J
Laboratory Medicine Center, Affiliated Hospital of Nantong University, The Jiangsu Province Key Laboratory of Neuroregeneration, Nantong University, Nantong 226001, People's Republic of China.
Wang
HuiMin
H
Yang
Huiguang
H
Huang
Xiaodong
X
Zhu
Jianchun
J
Hu
Ling
L
Qian
Ji
J
Yang
Junling
J
Zhou
Dan
D
Ni
Xiaohui
X
Shen
Aiguo
A
Cheng
Chun
C
D000818:Animals; D001921:Brain / Q000166:cytology; D002478:Cells, Cultured; D004622:Embryo, Mammalian; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005700:Galactosyltransferases / Q000235:genetics / Q000378:metabolism*; D005786:Gene Expression Regulation / Q000187:drug effects; D005911:Gliosis / Q000139:chemically induced* / Q000378:metabolism*; D006023:Glycoproteins / Q000235:genetics / Q000378:metabolism; D037102:Lectins / Q000378:metabolism; D008070:Lipopolysaccharides; D008297:Male; D017628:Microglia / Q000187:drug effects; D009457:Neuroglia / Q000187:drug effects; D009474:Neurons / Q000187:drug effects; D010587:Phagocytosis / Q000187:drug effects; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D013997:Time Factors
D006023:Glycoproteins; D037102:Lectins; D008070:Lipopolysaccharides; D012333:RNA, Messenger; D005700:Galactosyltransferases; C119701:beta-1,4-galactosyltransferase I