title: Investigation of the elasticity of a cisplatin-DNA adduct via single-molecule measurements and bimodal modeling.
abstract: Cisplatin has been known as an anticancer drug for a long time. It is therapeutically active upon binding to DNA. A double-bound cisplatin bends DNA into a localized kink. We model the elastic properties of cisplatin-DNA adducts at moderate tension (<6 pN). It is shown that from the mechanical point of view the action of cisplatin can be revealed by reduced persistence length. We derived two expressions for the persistence length, which apply in the linear-response and the strong-force regimes, respectively. Experimental data for DNA adducts stretched by magnetic tweezers are consistently fitted by these expressions. This allows us to estimate the degree of platination at various salt concentrations.
authors.affiliation: Department of Physics, Institute of Fundamental Physics, Sejong University, Seoul 143-743, Korea. lee@sejong.ac.kr
authors.forename: Nam-Kyung
authors.initials: NK
authors.lastname: Lee
authors.forename: Jin-Sung
authors.initials: JS
authors.lastname: Park
authors.forename: Albert
authors.initials: A
authors.lastname: Johner
authors.forename: Sergei
authors.initials: S
authors.lastname: Obukhov
authors.forename: Ju-Yong
authors.initials: JY
authors.lastname: Hyon
authors.forename: Kyoung J
authors.initials: KJ
authors.lastname: Lee
authors.forename: Seok-Cheol
authors.initials: SC
authors.lastname: Hong
mesh_terms: D000465:Algorithms; D000970:Antineoplastic Agents / Q000737:chemistry; D002945:Cisplatin / Q000737:chemistry*; D004247:DNA / Q000737:chemistry; D018736:DNA Adducts / Q000737:chemistry*; D004548:Elasticity; D008958:Models, Molecular
chemical_list: D000970:Antineoplastic Agents; D018736:DNA Adducts; C057270:cisplatin-DNA adduct; D004247:DNA; D002945:Cisplatin