[Lipid-associated membrane lipopeptides of M. arginini activate NF-kB by interacting with TLR2/1, TLR2/6, and TLR2/CD14].
Various strains of mycoplasmas cause activation of transcriptional factor NF-kB as a result of interaction with different combinations of Toll-like receptors (TLR). It is well known that the MALP-2 protein of M. fermentans activates the NF-kB through interaction with the TLR2/6, lipid-associated membrane lipopeptides (LAMPs) of M. penetrans through the TLR1/2, LAMPs of M. pneumoniae through combinations of Toll-like receptors (TLR2/6 and TLR1/2), and superantigene of M. arthritidis through the TLR2 and TLR4-dependent pathways. In this study, we defined specific Toll-like receptors for LAMPs of M. arginini. For carrying out the research we used cell lines 293-null, 293-hTLR2, 293-hTLR1/2, 293-hTLR2/CD14, 293-hTLR2/6, 293-hTLR4/ CD14-MD2 expressing certain combinations of TLR and their coreceptors. It was shown that LAMPs of M. arginini cause activation of NF-kB interacting with TLR2/1, TLR2/6 and TLR2/ CD14, but not with TLR2 alone or TLR4.
Logunov
D Iu
DIu
Shchebliakov
D V
DV
Zubkova
O V
OV
Shmarov
M M
MM
Rakovskaia
I V
IV
Gintsburg
L A
LA
Gudkov
A V
AV
Naroditskiĭ
B S
BS
D001426:Bacterial Proteins / Q000378:metabolism*; D002461:Cell Line, Transformed; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins; D055666:Lipopeptides / Q000378:metabolism*; D008565:Membrane Proteins / Q000378:metabolism*; D009174:Mycoplasma / Q000378:metabolism* / Q000472:pathogenicity; D016328:NF-kappa B / Q000378:metabolism*; D010455:Peptides / Q000378:metabolism* / Q000494:pharmacology; D051193:Toll-Like Receptors / Q000378:metabolism*; D015854:Up-Regulation
D001426:Bacterial Proteins; D036341:Intercellular Signaling Peptides and Proteins; D055666:Lipopeptides; D008565:Membrane Proteins; D016328:NF-kappa B; D010455:Peptides; D051193:Toll-Like Receptors; C043948:lipid-associating peptides