miR-210 promotes osteoblastic differentiation through inhibition of AcvR1b.
Although microRNAs (miRNAs) are involved in many biological processes, the mechanisms whereby miRNAs regulate osteoblastic differentiation are poorly understood. Here, we found that BMP-4-induced osteoblastic differentiation of bone marrow-derived ST2 stromal cells was promoted and repressed after transfection of sense and antisense miR-210, respectively. A reporter assay demonstrated that the activin A receptor type 1B (AcvR1b) gene was a target for miR-210. Furthermore, inhibition of transforming growth factor-beta (TGF-beta)/activin signaling in ST2 cells with SB431542 promoted osteoblastic differentiation. We conclude that miR-210 acts as a positive regulator of osteoblastic differentiation by inhibiting the TGF-beta/activin signaling pathway through inhibition of AcvR1b.
Mizuno
Yosuke
Y
Division of Functional Genomics and Systems Medicine, Research Center for Genomic Medicine, Saitama Medical University, Hidaka, Japan.
Tokuzawa
Yoshimi
Y
Ninomiya
Yuichi
Y
Yagi
Ken
K
Yatsuka-Kanesaki
Yukiko
Y
Suda
Tatsuo
T
Fukuda
Toru
T
Katagiri
Takenobu
T
Kondoh
Yasumitsu
Y
Amemiya
Tomoyuki
T
Tashiro
Hideo
H
Okazaki
Yasushi
Y
D030201:Activin Receptors, Type I / Q000037:antagonists & inhibitors* / Q000378:metabolism; D000818:Animals; D055415:Bone Morphogenetic Protein 4 / Q000378:metabolism; D002454:Cell Differentiation; D002478:Cells, Cultured; D051379:Mice; D035683:MicroRNAs / Q000378:metabolism*; D010006:Osteoblasts / Q000166:cytology* / Q000378:metabolism; D015398:Signal Transduction; D014162:Transfection; D016212:Transforming Growth Factor beta / Q000378:metabolism
D055415:Bone Morphogenetic Protein 4; D035683:MicroRNAs; D016212:Transforming Growth Factor beta; D030201:Activin Receptors, Type I