Characterization and antineoplasic effect of extracts obtained from Pleurotus sajor-caju fruiting bodies.
Fungi of the Pleurotus genus present a great industrial interest due to their possibility of producing pharmacological compounds, pigments, aromas, organic acids, polysaccharides, enzymes, vitamins, amino acids, etc. Among the therapeutic products, we can highlight those with antineoplasic activity, attributed to the fungi cell wall components. Based on this, the objective of this work was to study the antineoplasic capacity of the polysaccharidic fractions obtained from Pleurotus sajor-caju fruiting bodies. Female Swiss mice were inoculated with the Ehrlich ascitic tumor (5 x 10(6) cells/animal) in ascitic form. The polysaccharidic fractions were administered intraperitoneally, during a 6-day period. Fractions FI and FII presented a lower volume of ascitic liquid (3.1 and 1.8 mL, respectively) and a higher reduction in the number of neoplasic cells present in the ascitic liquid (86.2% and 85%, respectively), when compared to the positive control (group inoculated with the tumor but without treatment). These fractions were characterized in terms of monosaccharide composition. Glucose was the major component detected, followed by galactose and mannose. The anomeric carbon configuration of the beta-glucan was confirmed by the (13)C NMR (delta 103.7). Substituted and free C3 and C6 were also detected. Protein bands were confirmed through infrared analysis.
Dalonso
Nicole
N
University of the Region of Joinville-UNIVILLE, Campus Universitário, s/n Bom Retiro, 89201-974, Joinville, Santa Catarina, Brazil.
Souza
Rafaela
R
Silveira
Márcia Luciane Lange
ML
Ruzza
Angelo Adolfo
AA
Wagner
Theodoro Marcel
TM
Wisbeck
Elisabeth
E
Furlan
Sandra Aparecida
SA
D000328:Adult; D000818:Animals; D000970:Antineoplastic Agents / Q000737:chemistry / Q000627:therapeutic use*; D001835:Body Weight; D002286:Carcinoma, Ehrlich Tumor / Q000188:drug therapy* / Q000473:pathology; D002457:Cell Extracts / Q000737:chemistry / Q000627:therapeutic use*; D002648:Child; D005260:Female; D048690:Fruiting Bodies, Fungal / Q000737:chemistry*; D006801:Humans; D051379:Mice; D009368:Neoplasm Transplantation; D019906:Nuclear Magnetic Resonance, Biomolecular; D020076:Pleurotus / Q000737:chemistry / Q000166:cytology; D011134:Polysaccharides / Q000737:chemistry / Q000627:therapeutic use; D017550:Spectroscopy, Fourier Transform Infrared
D000970:Antineoplastic Agents; D002457:Cell Extracts; D011134:Polysaccharides