Ginsenoside Rg1 protects rat cardiomyocyte from hypoxia/reoxygenation oxidative injury via antioxidant and intracellular calcium homeostasis.
Ginsenoside Rg1 is a major active ingredient of Panax notoginseng radix which has demonstrated a number of pharmacological actions including a cardioprotective effect in vivo. This study investigated the protective effect and mechanism of ginsenoside Rg1 in cardiomyocytes hypoxia/reoxygenation (H/R) model. Pretreatment with ginsenoside Rg1 (60-120 microM) reduced lactate dehydrogenase release and increased cell viability in a dose-dependent manner. Fluorescence analysis demonstrated ginsenoside Rg1 reduced intracellular ROS and suppressed the intracellular [Ca(2+)] level. Cell lysate detected an increase of T-SOD, CAT, and GSH levels. The myocardial protection of ginsenoside Rg1 during H/R is partially due to its antioxidative effect and intracellular calcium homeostasis.
Zhu
Dan
D
Beijing Institute of Radiation Medicine, Beijing 100850, PR China.
Wu
Lei
L
Li
Chun-Rong
CR
Wang
Xiao-Wei
XW
Ma
Yong-Jie
YJ
Zhong
Zhi-Yin
ZY
Zhao
Hai-Bao
HB
Cui
Jian
J
Xun
Si-Fan
SF
Huang
Xiu-Lan
XL
Zhou
Zhe
Z
Wang
Sheng-Qi
SQ
D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D002118:Calcium / Q000378:metabolism*; D015687:Cell Hypoxia; D002470:Cell Survival; D005260:Female; D036145:Ginsenosides / Q000494:pharmacology*; D006706:Homeostasis; D008297:Male; D032383:Myocytes, Cardiac / Q000187:drug effects* / Q000378:metabolism; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D010100:Oxygen / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D017382:Reactive Oxygen Species / Q000378:metabolism
D000975:Antioxidants; D036145:Ginsenosides; D017382:Reactive Oxygen Species; C035054:ginsenoside Rg1; D010100:Oxygen; D002118:Calcium