title: Engineering and improvement of the efficiency of a chimeric [P450cam-RhFRed reductase domain] enzyme.
abstract: A chimeric oxygenase, in which the P450cam domain was fused to the reductase host domains of a P450RhF from Rhodococcus sp. strain NCIMB 9784 was optimised to allow for a biotransformation at 30 mM substrate in 80% overall yield, with the linker region between P450 and FMN domain proving to be important for the effective biotransformation of (+)-camphor to 5-exo-hydroxycamphor.
authors.affiliation: Manchester Interdisciplinary Biocentre, University of Manchester, UK.
authors.forename: Aélig
authors.initials: A
authors.lastname: Robin
authors.forename: Gareth A
authors.initials: GA
authors.lastname: Roberts
authors.forename: Johannes
authors.initials: J
authors.lastname: Kisch
authors.forename: Federico
authors.initials: F
authors.lastname: Sabbadin
authors.forename: Gideon
authors.initials: G
authors.lastname: Grogan
authors.forename: Neil
authors.initials: N
authors.lastname: Bruce
authors.forename: Nicholas J
authors.initials: NJ
authors.lastname: Turner
authors.forename: Sabine L
authors.initials: SL
authors.lastname: Flitsch
mesh_terms: D019475:Camphor 5-Monooxygenase / Q000737:chemistry* / Q000235:genetics*; D010088:Oxidoreductases / Q000737:chemistry* / Q000235:genetics*; D015202:Protein Engineering / Q000379:methods*; D017434:Protein Structure, Tertiary; D011993:Recombinant Fusion Proteins / Q000737:chemistry* / Q000235:genetics*; D012240:Rhodococcus / Q000201:enzymology; D014170:Transformation, Genetic
chemical_list: D011993:Recombinant Fusion Proteins; D010088:Oxidoreductases; D019475:Camphor 5-Monooxygenase