Prevalence and diversity of extended-spectrum beta-lactamases in faecal Escherichia coli isolates from healthy humans in Spain.
Extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli isolates were detected in seven of 105 faecal samples from healthy humans, from two Spanish cities, during 2007. In these isolates, five ESBLs were detected, CTX-M-14 (n = 2), CTX-M-1 (n = 2), CTX-M-32 (n = 1), CTX-M-8 (n = 1) and TEM-52 (n = 1). Both bla(CTX-M-14a) (surrounded by ISEcp1-IS903) and bla(CTX-M-14b) variants (included in an integron structure) were identified in this study. This is the first time that the bla(CTX-M-8) gene and ESBLs of the CTX-M-8 group have been found in Europe and Spain, respectively. Faecal E. coli of healthy humans therefore constitute a reservoir of bla(CTX-M) genes with different surrounding genetic elements.
Vinué
L
L
Area de Bioquímica y Biología Molecular, Universidad de La Rioja, Logroño, Spain.
Sáenz
Y
Y
Martínez
S
S
Somalo
S
S
Moreno
M A
MA
Torres
C
C
Zarazaga
M
M
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002648:Child; D002675:Child, Preschool; D004269:DNA, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000201:enzymology* / Q000302:isolation & purification*; D029968:Escherichia coli Proteins / Q000096:biosynthesis* / Q000145:classification* / Q000235:genetics; D005243:Feces / Q000382:microbiology*; D005260:Female; D023061:Gene Order; D006801:Humans; D008297:Male; D008826:Microbial Sensitivity Tests; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D013030:Spain; D055815:Young Adult; D001618:beta-Lactamases / Q000096:biosynthesis* / Q000145:classification* / Q000235:genetics; D047090:beta-Lactams / Q000494:pharmacology
D004269:DNA, Bacterial; D029968:Escherichia coli Proteins; D047090:beta-Lactams; D001618:beta-Lactamases