title: Highly selective and simple method for determination of polythiols based on liquid chromatography with postcolumn excimer fluorescence derivatization.
abstract: An LC postcolumn derivatization method for determination of polythiols has been developed. This method involves separation using reversed-phase LC, postcolumn derivatization with N-(1-pyrenyl)maleimide, and excimer fluorescence detection. Analytes with a polythiol structure were converted into corresponding polypyrene-labeled derivatives, and the derivatives exhibited intramolecular excimer fluorescence (440-520 nm). In this study, dimercaprol and dithiothreitol were used as model polythiols. This polythiol analysis method is simple; it is also highly selective and sensitive and yields good calibration curves.
authors.affiliation: Faculty of Pharmaceutical Sciences, Fukuoka University, 8-19-1 Nanakuma, Johnan, Fukuoka 814-0180, Japan.
authors.forename: Hideyuki
authors.initials: H
authors.lastname: Yoshida
authors.forename: Maki
authors.initials: M
authors.lastname: Sudo
authors.forename: Kenichiro
authors.initials: K
authors.lastname: Todoroki
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Nohta
authors.forename: Masatoshi
authors.initials: M
authors.lastname: Yamaguchi
mesh_terms: D002853:Chromatography, Liquid / Q000295:instrumentation / Q000379:methods*; D005453:Fluorescence; D011108:Polymers / Q000032:analysis*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013438:Sulfhydryl Compounds / Q000032:analysis*; D013997:Time Factors
chemical_list: D011108:Polymers; D013438:Sulfhydryl Compounds