title: Measurement of sulfur-containing compounds involved in the metabolism and transport of cysteamine and cystamine. Regional differences in cerebral metabolism.
abstract: An HPLC method with coulometric detection is presented for the quantitation of cysteamine, cystamine, thialysine, glutathione, glutathione disulfide and an oxidized metabolite of thialysine [S-(2-aminoethyl)-L-cysteine ketimine decarboxylated dimer (AECK-DD)]. The advantage of coulometric detection is that derivatization is unnecessary if the analyte is redox sensitive. The method was used to quantitate several sulfur-containing compounds in plasma and brain following gavage feeding of cysteamine to rats. Cysteamine, cystamine, thialysine and AECK-DD were detected in the brains of these animals. Interestingly, cysteamine treatment resulted in greatly elevated levels of cerebral methionine, despite the fact that cysteamine is not a precursor of methionine.
authors.affiliation: Department of Biochemistry and Molecular Biology, New York Medical College, Valhalla, NY 10595, USA.
authors.forename: John T
authors.initials: JT
authors.lastname: Pinto
authors.forename: Tetyana
authors.initials: T
authors.lastname: Khomenko
authors.forename: Sandor
authors.initials: S
authors.lastname: Szabo
authors.forename: Gordon D
authors.initials: GD
authors.lastname: McLaren
authors.forename: Travis T
authors.initials: TT
authors.lastname: Denton
authors.forename: Boris F
authors.initials: BF
authors.lastname: Krasnikov
authors.forename: Thomas M
authors.initials: TM
authors.lastname: Jeitner
authors.forename: Arthur J L
authors.initials: AJ
authors.lastname: Cooper
mesh_terms: D000818:Animals; D001692:Biological Transport; D054022:Cerebrum / Q000737:chemistry / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D003538:Cystamine / Q000378:metabolism*; D003543:Cysteamine / Q000378:metabolism*; D008297:Male; D010084:Oxidation-Reduction; D051381:Rats; D017207:Rats, Sprague-Dawley; D013457:Sulfur Compounds / Q000032:analysis* / Q000097:blood / Q000378:metabolism
chemical_list: D013457:Sulfur Compounds; D003543:Cysteamine; D003538:Cystamine