Butyrate reduces the frequency of micronuclei in human colon carcinoma cells in vitro.
Butyrate, formed by bacterial fermentation of plant foods, has been shown to protect human colon cells from selected genotoxic substances. The mechanism for this effect could be the enhancement of toxicological defence leading to an increased detoxification of genotoxic risk factors and thus to a reduction of DNA and chromosome damage. Previous protective properties of butyrate against DNA damage induction in colon cells were demonstrated using the comet assay. In the present study the effect of butyrate on chromosome damage induced by ferric nitrilotriacetate (Fe-NTA) and hydrogen peroxide (H(2)O(2)) (suggested to be putative risk factors of colorectal carcinogenesis) was investigated using the cytokinesis-block micronucleus (CBMN) test. It was possible to reveal that pre-treatment of HT29 colon carcinoma cells with butyrate (2 and 4mM) for 15 min caused a reduction of micronuclei induced with H(2)O(2) (75 microM; p<0.01) and Fe-NTA (500 and 1000 microM; p<0.05). The decrease in the level of Fe-NTA- and H(2)O(2)-induced micronuclei was also confirmed in most of the corresponding variants of 24h pre-treatment of cells with butyrate. The results obtained demonstrate for the first time protective properties of butyrate against chromosome damage induced by H(2)O(2) and Fe-NTA in human colon carcinoma cells.
Hovhannisyan
Galina
G
Department of Genetics and Cytology, Biological Faculty, State University, Yerevan 375025, Armenia.
Aroutiounian
Rouben
R
Glei
Michael
M
D000230:Adenocarcinoma / Q000378:metabolism; D002087:Butyrates / Q000008:administration & dosage / Q000494:pharmacology*; D003110:Colonic Neoplasms / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D005260:Female; D005290:Ferric Compounds / Q000008:administration & dosage / Q000633:toxicity; D019073:HT29 Cells; D006801:Humans; D006861:Hydrogen Peroxide / Q000008:administration & dosage / Q000633:toxicity; D048629:Micronuclei, Chromosome-Defective / Q000187:drug effects*; D015162:Micronucleus Tests; D009571:Nitrilotriacetic Acid / Q000008:administration & dosage / Q000031:analogs & derivatives / Q000633:toxicity
D002087:Butyrates; D005290:Ferric Compounds; D006861:Hydrogen Peroxide; D009571:Nitrilotriacetic Acid; C020326:ferric nitrilotriacetate