Establishment of mouse erythroleukemia cell lines expressing complete Influenza C virus CM2 protein or chimeric protein consisting of CM2 and Influenza A virus M2.
The role of the Influenza C virus (ICV) CM2 protein in virus replication as well as its precise function as an ion channel remains to be elucidated. For this purpose, we established a CM2-expressing mouse erythroleukemia (MEL) cell line and determined the biochemical characteristics of the expressed CM2. The features of the expressed CM2 were similar to those of the viral CM2 synthesized in ICV-infected cells. Furthermore, we established MEL cell line expressing a chimeric protein consisting of characteristic regions of CM2 and Influenza A virus (IAV) M2 protein that could be helpful in elucidation of the specific ion conductance properties.
Muraki
Y
Y
Division of Virology, National Institute for Medical Research, London, UK. ymuraki@med.id.yamagata-u.ac.jp
Hay
A
A
D000818:Animals; D002461:Cell Line, Transformed; D045744:Cell Line, Tumor; D006801:Humans; D009980:Influenza A virus / Q000235:genetics / Q000378:metabolism; D009982:Gammainfluenzavirus / Q000235:genetics / Q000378:metabolism; D004915:Leukemia, Erythroblastic, Acute / Q000821:virology; D051379:Mice; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism*; D014763:Viral Matrix Proteins / Q000235:genetics / Q000378:metabolism*; D014773:Virology / Q000379:methods; D000086082:Viroporin Proteins
D011993:Recombinant Fusion Proteins; D014763:Viral Matrix Proteins; C090877:CM2 protein, Influenza C virus; C488937:M2 protein, Influenza A virus; D000086082:Viroporin Proteins