title: Induced pluripotent stem cells at nanoscale.
abstract: Reprogramming of mouse and human somatic cells into induced pluripotent stem (iPS) cells has been made possible with the expression of the transcription factor quartet Oct4, Sox2, c-Myc, and Klf4. Here, we compared iPS cells derived from mouse embryonic fibroblasts with the 4 factors to embryonic stem cells by electron microscopy. Both cell types are almost indistinguishable at the ultrastructural level, providing further evidence for the similarity of these 2 pluripotent stem cell populations.
authors.affiliation: Department of Cell and Developmental Biology, Max Planck Institute for Molecular Biomedicine , Münster, Germany.
authors.forename: Dagmar
authors.initials: D
authors.lastname: Zeuschner
authors.forename: Karina
authors.initials: K
authors.lastname: Mildner
authors.forename: Holm
authors.initials: H
authors.lastname: Zaehres
authors.forename: Hans R
authors.initials: HR
authors.lastname: Schöler
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D065150:Cellular Reprogramming / Q000187:drug effects*; D053595:Embryonic Stem Cells / Q000166:cytology; D005347:Fibroblasts / Q000166:cytology; D057026:Induced Pluripotent Stem Cells / Q000166:cytology*; D000090062:Kruppel-Like Factor 4; D051741:Kruppel-Like Transcription Factors / Q000494:pharmacology; D051379:Mice; D008854:Microscopy, Electron; D050814:Octamer Transcription Factor-3 / Q000494:pharmacology; D016271:Proto-Oncogene Proteins c-myc / Q000494:pharmacology; D055748:SOXB1 Transcription Factors / Q000494:pharmacology; D014157:Transcription Factors / Q000494:pharmacology*
chemical_list: C000715467:KLF4 protein, human; C000715468:Klf4 protein, mouse; D000090062:Kruppel-Like Factor 4; D051741:Kruppel-Like Transcription Factors; C489428:Myc protein, mouse; D050814:Octamer Transcription Factor-3; C494174:Pou5f1 protein, mouse; D016271:Proto-Oncogene Proteins c-myc; D055748:SOXB1 Transcription Factors; C527783:Sox2 protein, mouse; D014157:Transcription Factors