A novel method developed for acetylcholine detection in royal jelly by using capillary electrophoresis coupled with electrogenerated chemiluminescence based on a simple reaction.
A novel method for highly sensitive detection of acetylcholine in royal jelly was proposed by using CE coupled with electrogenerated chemiluminescence (ECL). Acetylcholine, which could not react with Tris(2,2'-bipyridine)ruthenium(II) to strengthen its ECL signals, decomposed into trimethylamine and strengthened the ECL signals sharply when it was heated to its melting point. This reaction needed no additional reagent and it was mild, simple, stable and rapid, without any side reaction. By combining the above process with CE separation technique, trimethylamine in royal jelly was completely separated from interfering substances and was successfully detected within 4 min. The limit of detection for acetylcholine was found to be 6.3 x 10(-8) g/mL with a signal-to-noise ratio of 3:1. Acetylcholine in the royal jelly was detected to be 912+/-58 microg/g. The recoveries of acetylcholine chloride in the sample were in the ranges of 92-106%. The coefficients of variation for intra-day and inter-day reproducibility were equal to or less than 4.9 and 6.8%, respectively.
Wei
Wei
W
School of Public Health, Southeast University, Dingjiaqiao, Nanjing, Jiangsu Province, P. R. China. wei_wei98@163.com
Wei
Min
M
Kang
Xuejun
X
Deng
Huihua
H
Lu
Zuhong
Z
D015082:2,2'-Dipyridyl / Q000031:analogs & derivatives / Q000737:chemistry; D000109:Acetylcholine / Q000032:analysis*; D019075:Electrophoresis, Capillary / Q000379:methods*; D005227:Fatty Acids / Q000737:chemistry*; D008163:Luminescent Measurements / Q000379:methods*; D008744:Methylamines / Q000032:analysis; D009942:Organometallic Compounds / Q000737:chemistry; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D000099219:Royal Jelly
D015082:2,2'-Dipyridyl; D000109:Acetylcholine; D005227:Fatty Acids; D008744:Methylamines; D009942:Organometallic Compounds; D000099219:Royal Jelly; C103599:bis(bipyridyl)ruthenium(II); C023336:trimethylamine