Molecular variability of five Cucumber mosaic virus isolates from China.
Cucumber mosaic virus (CMV) isolates are currently divided into two main groups, I and II according to their genomic sequences. The group I is further divided into two subgroups IA and IB. We performed a phylogenetic analysis of the genome regions containing 1a, 2a, 2b, coat protein (CP), and movement protein (MP) genes of 5 CMV isolates from China and other 28 CMV isolates available in the GenBank. The results indicated that CMV isolates could be genetically divided into three groups I, II, and III according to the genes encoding MP, CP, 1a, and 2a proteins and to the 2 groups according to the gene 2b. Group I could be further divided into two subgroups (IA and IB) according to the genes encoding CP, MP, 2a, and 2b proteins and to the three subgroups (IA, IB, and IC) according to the gene encoding 1a protein. Four of 5 examined Chinese CMV isolates belonged to the subgroup IB, while the remaining isolate was a natural inter-subgroup reassortant. We found that the 2b gene of CMV was under positive selection, while the other genes were under negative selection. No evidence of the selection associated with a host adaptation or geographic distribution was found.
Liu
Y Y
YY
Department of Plant Pathology, College of Plant Protection, Shandong Agricultural University, Shandong, PR China.
Yu
S L
SL
Lan
Y F
YF
Zhang
C L
CL
Hou
S S
SS
Li
X D
XD
Chen
X Z
XZ
Zhu
X P
XP
D036022:Capsid Proteins / Q000235:genetics*; D002681:China; D003001:Cloning, Molecular; D017799:Cucumovirus / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D014644:Genetic Variation; D008969:Molecular Sequence Data; D032306:Petunia / Q000821:virology; D010802:Phylogeny; D010935:Plant Diseases / Q000821:virology; D053830:Plant Viral Movement Proteins / Q000235:genetics*; D016865:Reassortant Viruses / Q000235:genetics; D011995:Recombination, Genetic; D017422:Sequence Analysis, DNA; D014026:Nicotiana / Q000821:virology
D036022:Capsid Proteins; D053830:Plant Viral Movement Proteins