title: Diffraction phase and fluorescence microscopy.
abstract: We have developed diffraction phase and fluorescence (DPF) microscopy as a new technique for simultaneous quantitative phase imaging and epi-fluorescence investigation of live cells. The DPF instrument consists of an interference microscope, which is incorporated into a conventional inverted fluorescence microscope. The quantitative phase images are characterized by sub-nanometer optical path-length stability over periods from milliseconds to a cell lifetime. The potential of the technique for quantifying rapid nanoscale motions in live cells is demonstrated by experiments on red blood cells, while the composite phase-fluorescence imaging mode is exemplified with mitotic kidney cells.
authors.forename: Yongkeun
authors.initials: Y
authors.lastname: Park
authors.forename: Gabriel
authors.initials: G
authors.lastname: Popescu
authors.forename: Kamran
authors.initials: K
authors.lastname: Badizadegan
authors.forename: Ramachandra R
authors.initials: RR
authors.lastname: Dasari
authors.forename: Michael S
authors.initials: MS
authors.lastname: Feld