Association of renal damage and oxidative stress in a rat model of metabolic syndrome. Influence of gender.
This study investigated the association between nephropathy and oxidative stress, by measurement of systolic blood pressure, lipid peroxidation, activities of catalase, manganese- and copper-zinc-superoxide dismutase and endothelial nitric oxide synthase expression and concentrations of nitrates/nitrites in kidneys from rats with Metabolic Syndrome. Weaning female or male rats had 30% sucrose to drink for 24 weeks (Metabolic Syndrome). Modulation by sex hormones was investigated by gonadectomy and hormone replacement. In Metabolic Syndrome, Castrated Metabolic Syndrome + Testosterone males and Ovariectomized Metabolic Syndrome females had increased blood pressure, proteinuria and lipid peroxidation. Nitrates/nitrites and activities of catalase, manganese and copper-zinc-superoxide dismutase decreased vs intact Control, Castrated Metabolic Syndrome males, intact Metabolic Syndrome and Ovariectomized Metabolic Syndrome + Estradiol females. The results suggest that sex hormones modulate the activity of superoxide-dismutase, catalase and endothelial nitric oxide-synthase. Ovariectomy decreased the protection against oxidative stress in females; the opposite occurred in castrated males.
Pérez-Torres
Israel
I
Department of Pathology, Instituto Nacional de Cardiología Ignacio Chávez, JuanBadiano 1, Sección XVI, Tlalpan, 14080 México DF, México.
Roque
Priscilla
P
El Hafidi
Mohammed
M
Diaz-Diaz
Eulises
E
Baños
Guadalupe
G
D000818:Animals; D002374:Catalase / Q000378:metabolism; D004958:Estradiol / Q000008:administration & dosage / Q000097:blood; D015914:Estrogen Replacement Therapy; D004967:Estrogens / Q000502:physiology*; D005260:Female; D020249:Hormone Replacement Therapy; D006973:Hypertension / Q000139:chemically induced; D007674:Kidney Diseases / Q000209:etiology* / Q000378:metabolism / Q000503:physiopathology; D015227:Lipid Peroxidation; D008297:Male; D024821:Metabolic Syndrome / Q000150:complications / Q000378:metabolism / Q000503:physiopathology*; D009566:Nitrates / Q000032:analysis; D052250:Nitric Oxide Synthase Type III / Q000378:metabolism; D009573:Nitrites / Q000032:analysis; D009919:Orchiectomy; D010052:Ovariectomy; D018384:Oxidative Stress; D051381:Rats; D017208:Rats, Wistar; D012727:Sex Characteristics; D013395:Sucrose / Q000633:toxicity; D013482:Superoxide Dismutase / Q000378:metabolism; D013739:Testosterone / Q000008:administration & dosage / Q000097:blood / Q000502:physiology*
D004967:Estrogens; D009566:Nitrates; D009573:Nitrites; D013739:Testosterone; D004958:Estradiol; D013395:Sucrose; D002374:Catalase; D052250:Nitric Oxide Synthase Type III; C496316:Nos3 protein, rat; D013482:Superoxide Dismutase