title: Use of Tomato leaf curl virus (TYLCV) truncated Rep gene sequence to engineer TYLCV resistance in tomato plants.
abstract: Tomato yellow leaf curl disease causes severe losses in tomato production throughout Mediterranean countries including Tunisia. In order to generate engineered resistance to this disease, an intron-hairpin RNA construct harboring a Tomato yellow leaf curl Sardinia virus (TYLCSV) truncated replication-associated protein (Rep) gene was used to transform genotype of tomato plants. Prepared transgenic plants were agro-inoculated with Tunisian infectious strain of TYLCSV and screened for the resistance to infection. The infected transgenic plants were divided into 3 different groups according to their specific symptoms. Only one of them contained transgenic plants fully resistant to the tomato yellow leaf curl disease.
authors.affiliation: Laboratory of Molecular Genetics, Immunology, and Biotechnology, Faculty of Science of Tunis, Tunis, Tunisia.
authors.forename: H
authors.initials: H
authors.lastname: Ben Tamarzizt
authors.forename: S
authors.initials: S
authors.lastname: Gharsallah Chouchane
authors.forename: R
authors.initials: R
authors.lastname: Lengliz
authors.forename: D P
authors.initials: DP
authors.lastname: Maxwell
authors.forename: M
authors.initials: M
authors.lastname: Marrakchi
authors.forename: H
authors.initials: H
authors.lastname: Fakhfakh
authors.forename: F
authors.initials: F
authors.lastname: Gorsane
mesh_terms: D053839:Begomovirus / Q000235:genetics / Q000472:pathogenicity*; D004265:DNA Helicases / Q000235:genetics*; D005818:Genetic Engineering / Q000379:methods; D018551:Solanum lycopersicum / Q000821:virology*; D010935:Plant Diseases / Q000821:virology; D018515:Plant Leaves / Q000821:virology; D030821:Plants, Genetically Modified; D034622:RNA Interference; D034741:RNA, Small Interfering; D015534:Trans-Activators / Q000235:genetics*; D014764:Viral Proteins / Q000235:genetics*
chemical_list: D034741:RNA, Small Interfering; D015534:Trans-Activators; D014764:Viral Proteins; C045264:replication initiator protein; D004265:DNA Helicases