title: Expressions of connexin and par-3 in the distal margin of rectal cancer after ultra-low anterior resection.
abstract: This study examined the expression of connexin and protease-activated receptor 3 (par-3) in the distal resection margin of rectal cancer and the correlation of the expression of the two proteins with tumor relapse. A total of 40 patients with rectal cancer underwent ultra-low anterior resection with curved cutter stapler. The pathological specimens were divided into 3 groups in terms of sampling sites: tumor group, 2.0-cm group (in which the tissues were harvested 2.0 cm distal to the tumor tissues), 3.0-cm group (in which the tissues were taken 3.0 cm away from the tumor tissues). All the samples were pathologically observed and then measured for the expression of connexin and par-3 by employing immunohistochemistry and Western blotting. The operations in this series went uneventfully. No anastomotic stoma bleeding, stenosis and death occurred postoperatively. Histopathologically, in the tumor group, epithelial cells lost normal pattern of arrangement and polarity, and were loosely connected and even detached. In the 3.0-cm group, the epithelia had normal appearance, obvious cell polarity and essentially intact cell junction. Immunohistochemistry and Western blotting indicated that the 3.0-cm group had the strongest expression of connexin and par-3, and the expression in the 2.0-cm group and the tumor group was relatively weak. There existed significant difference in the expression of the two proteins among the three groups (P<0.05 for all). It was concluded that the down-regulated connexin and par-3 in the distal margin of rectal cancer tissues may indicate the progression of the disease and high likelihood of recurrence and metastasis. Although no tumor cells were found in the sections of the 2.0-cm group, the decreased expression of connexin and par-3 may suggest the development of anaplasia and the increased odds of tumor relapse. Therefore, we are led to speculate that tumor resection only including 2.0 cm of unaffected rectum could not completely avoid the distant metastasis and local relapse.
authors.affiliation: Department of Gastroenterological Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China. liujunxh@yahoo.cn
authors.forename: Jun
authors.initials: J
authors.lastname: Liu
authors.forename: Weikang
authors.initials: W
authors.lastname: Zhang
authors.forename: Jinlin
authors.initials: J
authors.lastname: Liu
authors.forename: Xiaoming
authors.initials: X
authors.lastname: Lu
authors.forename: Yaoping
authors.initials: Y
authors.lastname: Long
authors.forename: Yancai
authors.initials: Y
authors.lastname: Zhou
authors.forename: Shenghong
authors.initials: S
authors.lastname: Liu
mesh_terms: D017630:Connexins / Q000235:genetics / Q000378:metabolism*; D013505:Digestive System Surgical Procedures / Q000379:methods; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009364:Neoplasm Recurrence, Local / Q000517:prevention & control; D018179:Receptors, Thrombin / Q000235:genetics / Q000378:metabolism*; D012004:Rectal Neoplasms / Q000378:metabolism* / Q000601:surgery; D012007:Rectum / Q000378:metabolism; D013528:Surgical Staplers
chemical_list: D017630:Connexins; D018179:Receptors, Thrombin; C105295:protease-activated receptor 3