KLOTHO gene polymorphisms G-395A and C1818T are associated with lipid and glucose metabolism, bone mineral density and systolic blood pressure in Japanese healthy subjects.
BACKGROUND
We investigated the association of KLOTHO gene single nucleotide polymorphisms (SNPs) with various laboratory data in 476 Japanese healthy subjects.


METHODS
The genotyping of G-395A in the promoter region and C1818T in exon 4 was performed using PCR with confronting 2-pair primers assay.


RESULTS
Multivariate analysis adjusted for age demonstrated that in men, body-fat ratio was high, and HDL cholesterol level was low in A allele carriers of G-395A compared with GG. In women, glucose was high in A allele carriers of G-395A compared with GG, and also in T allele carriers of C1818T compared with CC. When divided into 2 groups according to age, in men <60 y, body mass index, body-fat ratio and waist circumference were high in A carriers of G-395A compared with GG. In women <60 y, bone mineral density was high in A allele carriers of G-395A compared with GG, and systolic blood pressure and glucose were high in T carriers of C1818T compared with CC.


CONCLUSIONS
KLOTHO gene SNPs G-395A and C1818T are associated with lipid metabolism in men, and glucose metabolism, bone mineral density and systolic blood pressure in women.
Shimoyama
Yasuhiko
Y
Clinical Preventive Medicine, Nagoya University Graduate School of Medicine, 65 Tsurumai-cho, Nagoya 466-8550, Japan.
Nishio
Kazuko
K
Hamajima
Nobuyuki
N
Niwa
Toshimitsu
T
D000375:Aging / Q000235:genetics / Q000378:metabolism / Q000502:physiology; D044466:Asian People / Q000235:genetics*; D001794:Blood Pressure / Q000235:genetics*; D015519:Bone Density / Q000235:genetics*; D005091:Exons / Q000235:genetics; D005260:Female; D005838:Genotype; D005947:Glucose / Q000378:metabolism*; D005966:Glucuronidase / Q000235:genetics*; D006801:Humans; D007564:Japan; D000090265:Klotho Proteins; D050356:Lipid Metabolism / Q000235:genetics*; D008297:Male; D008593:Menopause / Q000235:genetics / Q000502:physiology; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D011401:Promoter Regions, Genetic / Q000235:genetics
D005966:Glucuronidase; D000090265:Klotho Proteins; D005947:Glucose