Identification and comparative analysis of the structural proteomes of phiKZ and EL, two giant Pseudomonas aeruginosa bacteriophages.
Giant bacteriophages phiKZ and EL of Pseudomonas aeruginosa contain 62 and 64 structural proteins, respectively, identified by ESI-MS/MS on total virion particle proteins. These identifications verify gene predictions and delineate the genomic regions dedicated to phage assembly and capsid formation (30 proteins were identified from a tailless phiKZ mutant). These data form the basis for future structural studies and provide insights into the relatedness of these large phages. The phiKZ structural proteome strongly correlates to that of Pseudomonas chlororaphis bacteriophage 201phi2-1. Phage EL is more distantly related, shown by its 26 non-conserved structural proteins and the presence of genomic inversions.
Lecoutere
Elke
E
Division of Gene Technology, Katholieke Universiteit Leuven, Leuven, Belgium.
Ceyssens
Pieter-Jan
PJ
Miroshnikov
Konstantin A
KA
Mesyanzhinov
Vadim V
VV
Krylov
Victor N
VN
Noben
Jean-Paul
JP
Robben
Johan
J
Hertveldt
Kirsten
K
Volckaert
Guido
G
Lavigne
Rob
R
D020543:Proteome / Q000737:chemistry*; D017105:Pseudomonas Phages / Q000737:chemistry*; D011550:Pseudomonas aeruginosa / Q000821:virology; D021241:Spectrometry, Mass, Electrospray Ionization; D015678:Viral Structural Proteins / Q000737:chemistry*
D020543:Proteome; D015678:Viral Structural Proteins