Paeonol from Paeonia suffruticosa prevents TNF-alpha-induced monocytic cell adhesion to rat aortic endothelial cells by suppression of VCAM-1 expression.
Paeonol (2'-hydroxy-4'-methoxyacetophenone), the main active compound of the radix of Paeonia suffruticosa, has been reported to have a beneficial effect in prevention and treatment of cardiovascular diseases. Vascular cell adhesion molecule-1 (VCAM-1), plays a crucial role in case of early inflammatory responses, including atherosclerosis. In this study, we investigated the effect of paeonol on TNF-alpha-induced VCAM-1 expression in rat aortic endothelial cells (RAECs). The VCAM-1 expression in paeonol treated RAECs was measured. Paeonol inhibited TNF-alpha-induced VCAM-1 expression in a concentration-dependent manner. TNF-alpha induced p38 and extracellular signal-regulated kinase 1/2 (ERK1/2) activities that contributed to VCAM-1expression was obviously attenuated after pre-treating RAECs with paeonol. The decrease of VCAM-1 expression by paeonol pretreatment led to a reduction of monocytes adhesion to RAECs. Taken together, our results demonstrated that paeonol inhibited VCAM-1 expression by the attenuation of p38 and ERK1/2 signal transduction pathways. We concluded that paeonol had the potential therapeutic development for use in anti-inflammatory and vascular disorders.
Pan
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Anhui College of Traditional Chinese Medicine, 103 Meishan Road, Hefei, 230038 Anhui, PR China.
Dai
M
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D000098:Acetophenones / Q000494:pharmacology*; D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects; D002478:Cells, Cultured; D042783:Endothelial Cells / Q000187:drug effects*; D020935:MAP Kinase Signaling System / Q000187:drug effects; D009000:Monocytes / Q000187:drug effects*; D029596:Paeonia; D010936:Plant Extracts / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D014409:Tumor Necrosis Factor-alpha; D019010:Vascular Cell Adhesion Molecule-1 / Q000378:metabolism*
D000098:Acetophenones; D010936:Plant Extracts; D014409:Tumor Necrosis Factor-alpha; D019010:Vascular Cell Adhesion Molecule-1; C013638:paeonol