Paramagnetic labelling of proteins and oligonucleotides for NMR.
Paramagnetic effects offer a rich source of long-range structural restraints. Here we review current methods for site-specific tagging of proteins and oligonucleotides with paramagnetic molecules. The paramagnetic tags include nitroxide radicals and metal chelators. Particular emphasis is placed on tags suitable for site-specific and rigid attachment of lanthanide ions to macromolecules.
Su
Xun-Cheng
XC
The Australian National University, Canberra, Australia.
Otting
Gottfried
G
D004578:Electron Spin Resonance Spectroscopy / Q000379:methods*; D028581:Lanthanoid Series Elements / Q000737:chemistry; D008958:Models, Molecular; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods*; D009841:Oligonucleotides / Q000737:chemistry*; D011487:Protein Conformation; D011506:Proteins / Q000737:chemistry*; D013113:Spin Labels
D028581:Lanthanoid Series Elements; D009841:Oligonucleotides; D011506:Proteins; D013113:Spin Labels