In vitro and in vivo antifibrotic effects of rosmarinic acid on experimental liver fibrosis.
This study was carried out to investigate whether rosmarinic acid (RA) has antifibrotic effect on experimental liver fibrosis in vitro and in vivo and its possible mechanism. Culture of hepatic stellate cells (HSCs) determine proliferation and expression of transforming growth factor-beta1 (TGF-beta1), connective transforming growth factor (CTGF) and alpha-smooth muscle actin (alpha-SMA). In carbon tetrachloride (CCL(4))-induced rat liver fibrosis model, determined biochemical indicator, liver fibrosis grade and histopathological changes, immunohistochemical detected liver TGF-beta1 and CTGF expression. The results indicated that RA could inhibit HSCs proliferation, inhibit TGF-beta1, CTGF and alpha-SMA expression in cultured HSCs. It has marked evident in reducing fibrosis grade, ameliorating biochemical indicator and histopathological morphology, reducing liver TGF-beta1 and CTGF expression in CCL(4)-induced liver fibrosis. These findings suggest that RA has potentially conferring antifibrogenic effects.
Li
Gui-Sheng
GS
School of Pharmacy, Yantai University, 32# Qingquan Road, Laishan District, Yantai 264003, PR China.
Jiang
Wang-Lin
WL
Tian
Jing-Wei
JW
Qu
Gui-Wu
GW
Zhu
Hai-Bo
HB
Fu
Feng-Hua
FH
D000199:Actins / Q000378:metabolism; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology / Q000627:therapeutic use*; D002252:Carbon Tetrachloride Poisoning / Q000150:complications / Q000188:drug therapy*; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D002934:Cinnamates / Q000494:pharmacology / Q000627:therapeutic use*; D055513:Connective Tissue Growth Factor / Q000037:antagonists & inhibitors; D053630:Depsides / Q000494:pharmacology / Q000627:therapeutic use*; D004195:Disease Models, Animal; D055166:Hepatic Stellate Cells / Q000187:drug effects*; D008099:Liver / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D008103:Liver Cirrhosis / Q000188:drug therapy* / Q000209:etiology / Q000378:metabolism; D008297:Male; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology / Q000627:therapeutic use*; D051381:Rats; D017207:Rats, Sprague-Dawley; D053773:Transforming Growth Factor beta1 / Q000037:antagonists & inhibitors; D000097123:Rosmarinic Acid
D000199:Actins; D000975:Antioxidants; D002934:Cinnamates; D055513:Connective Tissue Growth Factor; D053630:Depsides; D010936:Plant Extracts; D053773:Transforming Growth Factor beta1; D000097123:Rosmarinic Acid