Expression of immune modulator cytokines in human fulminant amoebic colitis.
Human fulminant amoebic colitis (FAC) is characterized by ulceration and inflammation of the colon. The specific mixture of pro-inflammatory and anti-inflammatory cytokines may participate in either the host defense or in the pathogenesis of amoebic colitis. Therefore, we studied the expression of IL-8, IL-10, IL-4, TGF-beta and IFN-gamma in human FAC patients and controls through immunohistochemistry analysis. The number of cells expressing IL-8, IL-4 and IL-10 was significantly enhanced in all FAC samples compared to the control samples. However, the expression of TGF- beta in patients was low in the colonic mucosa and high in the lamina propria compared with the control. No expression of IFN-gamma was found in the controls or FAC samples. The production of IL-8 by intestinal epithelial cells may play a role in the pathogenesis of amoebic infection, because this cytokine attracts neutrophils, which lead to an inflammatory reaction that results in tissue damage. The predominant expression of the macrophage down-regulating cytokines, IL-4, IL-10 and TGF-beta, or the Th2-type immune response could inhibit a cell-mediated immune response, which in turn would facilitate parasite invasion in these tissues.
Sierra-Puente
R E
RE
Departamento de Histología, Facultad de Medicina, Universidad Juárez del Estado de Durango, Durango, México.
Campos-Rodríguez
R
R
Jarillo-Luna
R A
RA
Muñoz-Fernández
L
L
Rodríguez
M G
MG
Muñoz-Ortega
M H
MH
Ventura-Juárez
J
J
D003106:Colon / Q000276:immunology* / Q000469:parasitology* / Q000473:pathology; D016207:Cytokines / Q000096:biosynthesis*; D004404:Dysentery, Amebic / Q000276:immunology*; D020869:Gene Expression Profiling; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D007413:Intestinal Mucosa / Q000276:immunology* / Q000473:pathology
D016207:Cytokines