Phosphoconjugation and dephosphorylation reactions of steroid hormone in insects.
In insects, the major products of phase II metabolism of ecdysteroids, which include the molting hormone, are phosphate esters. The phosphoconjugation pathway is a reversible process, comprising two enzyme systems: ecdysteroid 22-kinase (EcKinase) and ecdysteroid-phosphate phosphatase (EPPase). We report here that: (1) the biochemical characteristics of EcKinase and EPPase, (2) the physiological significance of the reciprocal conversion of ecdysteroids and ecdysteroid phosphates in the ovary-egg system in insects, (3) the biochemical mechanism by which ecdysteroid phosphates are synthesized in the ovary, transferred to eggs, and finally dephosphorylated in eggs, and (4) the possible catalytic steps of EcKinase and EPPase on the basis of the data obtained by an in silico study. From these studies, it is obvious that ecdysteroid phosphates as well as steroid sulfates, which are major products of phase II metabolism in mammals, function as precursors for the formation of biologically active hormones.
Sonobe
Haruyuki
H
Department of Biology, Konan University, Higashinada-ku, Kobe, Japan. sonobe@konan-u.ac.jp
Ito
Yoichi
Y
D000818:Animals; D026461:Ecdysteroids / Q000378:metabolism*; D005260:Female; D007313:Insecta / Q000201:enzymology / Q000378:metabolism*; D010053:Ovary / Q000378:metabolism; D010744:Phosphoric Monoester Hydrolases / Q000737:chemistry; D010766:Phosphorylation; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000737:chemistry
D026461:Ecdysteroids; D017853:Phosphotransferases (Alcohol Group Acceptor); C476613:ecdysteroid-phosphate phosphatase; D010744:Phosphoric Monoester Hydrolases