title: Cytotoxic activity of Enterobacter cloacae human isolates.
abstract: We examined 55 Enterobacter cloacae isolates from clinical specimens for the production of cytotonic and cytotoxic toxins and the presence of the type III secretion system (TTSS). Twelve isolates (22%) revealed cytotoxic activity that caused destruction of Vero cells, whereas 28 (51%) strains induced lysis of the murine macrophage J774 cell line. TTSS genes were present in 27% of the isolates. The results indicated that these bacteria may destroy phagocytes and epithelial cells, which may lead to spread within the host.
authors.affiliation: Department of Microbiology, Faculty of Biology, A. Mickiewicz University, 61-614 Poznań, ul. Umultowska 89, Poland. sylkrzym@amu.edu.pl
authors.forename: Sylwia
authors.initials: S
authors.lastname: Krzymińska
authors.forename: Joanna
authors.initials: J
authors.lastname: Mokracka
authors.forename: Ryszard
authors.initials: R
authors.lastname: Koczura
authors.forename: Adam
authors.initials: A
authors.lastname: Kaznowski
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics; D001427:Bacterial Toxins / Q000633:toxicity*; D002460:Cell Line; D002522:Chlorocebus aethiops; D016972:Enterobacter cloacae / Q000302:isolation & purification / Q000472:pathogenicity*; D004756:Enterobacteriaceae Infections / Q000382:microbiology*; D006801:Humans; D026901:Membrane Transport Proteins / Q000235:genetics; D051379:Mice
chemical_list: D001426:Bacterial Proteins; D001427:Bacterial Toxins; D026901:Membrane Transport Proteins