title: Effect of different sample volumes on the DNA extraction of Aspergillus fumigatus from whole blood.
abstract: Five methods were compared, using conventional PCR, for the isolation of DNA from Aspergillus fumigatus conidia from 1-3-mL samples of whole blood. A lower detection threshold of Aspergillus conidia was achieved using 3-mL rather than 1-mL samples with three of five methods tested.
authors.affiliation: Division of Oncology and Haematology, Department of Medicine, Charité-Universitätsmedizin Berlin, Berlin, Germany.
authors.forename: B
authors.initials: B
authors.lastname: Schulz
authors.forename: K
authors.initials: K
authors.lastname: Weber
authors.forename: C
authors.initials: C
authors.lastname: Radecke
authors.forename: C
authors.initials: C
authors.lastname: Scheer
authors.forename: M
authors.initials: M
authors.lastname: Ruhnke
mesh_terms: D001228:Aspergillosis / Q000097:blood / Q000382:microbiology; D001232:Aspergillus fumigatus / Q000235:genetics / Q000302:isolation & purification*; D001800:Blood Specimen Collection / Q000379:methods*; D004271:DNA, Fungal / Q000097:blood* / Q000302:isolation & purification*; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D011933:Reagent Kits, Diagnostic
chemical_list: D004271:DNA, Fungal; D011933:Reagent Kits, Diagnostic