[Isolation and primary identification of viruses in mosquitoes in the south of Xinjiang].
OBJECTIVE
To isolate viruses from mosquitoes in the south of Xinjiang and identify these viruses primarily.


METHODS
A total of 13 491 mosquitoes were collected in the south of Xinjiang from Jul to Aug, 2005. These mosquitoes were divided into 130 groups and grinded respectively. The supernates were inoculated in C6/36 and Vero cells. Viruses isolated were detected, the genomic nucleic types by electrophoresis of viral genomes and the morphologies observed under electronmicroscope.


RESULTS
All 42 viruses were isolated, which caused CPEs on C6/36 but not on Vero cells. 27 viruses showed similar genomic profiles with 12 dsRNA segments. 1 virus displayed genomic profile with 10 dsRNA segments. 5 viruses took on similar genomic profiles with about 4 kbp DNA band. 9 viruses did not get any taxonomy information. Electromicroscopic pictures of these viruses revealed that above four types of viruses had distinguished morphologies indicating different virus species.


CONCLUSIONS
There should be several virus species in the mosquitoes in the south of Xinjiang. dsRNA virus with 12 genomic segments should play analysis a predominant role in the south of Xinjiang.
Lü
Xin-jun
XJ
Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 100052, China.
Lü
Zhi
Z
Sun
Xiao-hong
XH
Fu
Shi-hong
SH
Wang
Huan-qin
HQ
Tong
Su-xiang
SX
Zhang
Song
S
Liang
Guo-dong
GD
D000818:Animals; D001820:Bluetongue virus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D002681:China; D002522:Chlorocebus aethiops; D009033:Culicidae / Q000821:virology*; D003716:Dengue Virus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D016679:Genome, Viral; D007304:Insect Viruses / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D012330:RNA, Double-Stranded / Q000235:genetics; D012367:RNA, Viral / Q000235:genetics; D016865:Reassortant Viruses / Q000235:genetics; D017422:Sequence Analysis, DNA; D014709:Vero Cells
D012330:RNA, Double-Stranded; D012367:RNA, Viral