title: Biotransformation of ginsenoside Rf to Rh1 by recombinant beta-glucosidase.
abstract: An Aspergillus niger strain was isolated from the soil around ginseng fruit. In vitro enzyme assays showed that this strain had the ability to transform total ginsenosides (TGS) into several new products. In a further biochemical study, a beta-glucosidase gene isolated from this strain, bgl1, was expressed in Saccharomyces cerevisiae. His-tagged BGL1 protein (approximately 170 kD) showed the ability to transform ginsenoside Rf into Rh(1).
authors.affiliation: College of Chinese Medicinal Materials, Jilin Agricultural University, Changchun, 130118, China.
authors.forename: Chang-Chun
authors.initials: CC
authors.lastname: Ruan
authors.forename: Hao
authors.initials: H
authors.lastname: Zhang
authors.forename: Lian-Xue
authors.initials: LX
authors.lastname: Zhang
authors.forename: Zhi
authors.initials: Z
authors.lastname: Liu
authors.forename: Guang-Zhi
authors.initials: GZ
authors.lastname: Sun
authors.forename: Jun
authors.initials: J
authors.lastname: Lei
authors.forename: Yu-Xia
authors.initials: YX
authors.lastname: Qin
authors.forename: Yi-Nan
authors.initials: YN
authors.lastname: Zheng
authors.forename: Xiang
authors.initials: X
authors.lastname: Li
authors.forename: Hong-Yu
authors.initials: HY
authors.lastname: Pan
mesh_terms: D001234:Aspergillus niger / Q000201:enzymology; D002851:Chromatography, High Pressure Liquid; D036145:Ginsenosides / Q000737:chemistry / Q000378:metabolism*; D015394:Molecular Structure; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism; D001617:beta-Glucosidase / Q000235:genetics / Q000378:metabolism*
chemical_list: D036145:Ginsenosides; D011994:Recombinant Proteins; C425564:ginsenoside Rh1; D001617:beta-Glucosidase; C055328:ginsenoside Rf