Anti-inflammatory activity of Motherwort (Leonurus sibiricus L.).
Motherwort (MW), a Korean folk medicine, has been applied to treat inflammatory disease. However, its effect on inflammatory cytokine release from mast cells is not well known. We investigated the anti- inflammatory effect of MW on the secretion of inflammatory cytokine such as tumor necrosis factor (TNF)-alpha and interleukin (IL)-6 and IL-8 in human mast cell line (HMC-1). MW was treated in vitro before activation of HMC-1 cells with phorbol 12-myristate 13-acetate (PMA) plus calcium ionophore A23187. MW had no cytotoxic effects on HMC-1 cell viability. MW (1 mg/ml) inhibited PMA plus A23187-stimulated gene expression and production of TNF-alpha, IL-6, and IL-8. Stimulation with PMA plus A23187 induced NF-kappaB activation in HMC-1 cells, which was inhibited by MW (1 mg/ml). MW inhibited secretion of TNF-alpha, IL-6, and IL-8 possibly by inhibiting NF-kappaB activation. These results indicate that MW may be helpful in regulating inflammatory diseases.
Shin
Hye-Young
HY
College of Pharmacy, Woosuk University, Jeonbuk, South Korea.
Kim
Sang-Hyun
SH
Kang
Sin-Myoung
SM
Chang
Ik-Jun
IJ
Kim
Sang-Yong
SY
Jeon
Hoon
H
Leem
Kang-Hyun
KH
Park
Won-Hwan
WH
Lim
Jong-Pil
JP
Shin
Tae-Yong
TY
D000893:Anti-Inflammatory Agents / Q000378:metabolism / Q000494:pharmacology*; D000001:Calcimycin / Q000494:pharmacology; D006801:Humans; D007249:Inflammation / Q000188:drug therapy; D015850:Interleukin-6 / Q000378:metabolism; D016209:Interleukin-8 / Q000378:metabolism; D031332:Leonurus / Q000378:metabolism*; D008407:Mast Cells / Q000187:drug effects* / Q000276:immunology; D008519:Medicine, Traditional; D016328:NF-kappa B / Q000378:metabolism; D008517:Phytotherapy; D010936:Plant Extracts / Q000378:metabolism / Q000494:pharmacology*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
D000893:Anti-Inflammatory Agents; D015850:Interleukin-6; D016209:Interleukin-8; D016328:NF-kappa B; D010936:Plant Extracts; D014409:Tumor Necrosis Factor-alpha; D000001:Calcimycin; D013755:Tetradecanoylphorbol Acetate