title: Mass spectrometric quantification of MIF-1 in mouse brain by multiple reaction monitoring.
abstract: MIF-1 (Pro-Leu-Gly-NH(2)) has potent therapeutic effects in depression and Parkinson's disease, but its CNS sites of production are not yet clear. In this study, the concentration of MIF-1 in different brain regions was measured by the multiple reaction monitoring technique on a 4000 QTRAP mass spectrometer. The limit of quantification was 300 fg of MIF-1, and limit of detection was 60 fg. The low molecular weight fractions of tissue homogenates from different regions of mouse brain were analyzed. The concentration of MIF-1 ranged from 22+/-3 fg/microg protein in cerebral cortex to 930+/-60 fg/microg protein in the hypothalamus. Moderate concentrations were also detected in all other regions tested, including the striatum, thalamus, and hippocampus. By incubation of stable isotope-labeled oxytocin with tissue preparations, it was also confirmed that oxytocin at least partially contributed to the production of MIF-1 in the hypothalamus by action of peptidases. Regional differences were also found. The results are the first to show the ultrasensitive quantification of MIF-1 in different brain regions, and support the neuromodulatory actions of MIF-1 in the striatum.
authors.affiliation: Pennington Biomedical Research Center, Baton Rouge, LA 70808, USA.
authors.forename: Indu
authors.initials: I
authors.lastname: Kheterpal
authors.forename: Abba J
authors.initials: AJ
authors.lastname: Kastin
authors.forename: Sahana
authors.initials: S
authors.lastname: Mollah
authors.forename: Chuanhui
authors.initials: C
authors.lastname: Yu
authors.forename: Hung
authors.initials: H
authors.lastname: Hsuchou
authors.forename: Weihong
authors.initials: W
authors.lastname: Pan
mesh_terms: D000818:Animals; D001921:Brain / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005260:Female; D007031:Hypothalamus / Q000378:metabolism; D009075:MSH Release-Inhibiting Hormone / Q000378:metabolism*; D051379:Mice; D010121:Oxytocin / Q000378:metabolism; D053719:Tandem Mass Spectrometry
chemical_list: D010121:Oxytocin; D009075:MSH Release-Inhibiting Hormone