Accelerated type III secretion system 2-dependent enteropathogenesis by a Salmonella enterica serovar enteritidis PT4/6 strain.
Salmonella enterica subsp. I serovars Typhimurium and Enteritidis are major causes of enteric disease. The pathomechanism of enteric infection by serovar Typhimurium has been studied in detail. Serovar Typhimurium employs two pathways in parallel for triggering disease, i.e., the "classical" pathway, triggered by type III secretion system 1 (TTSS-1), and the "alternative" pathway, mediated by TTSS-2. It had remained unclear whether these two pathways would also explain the enteropathogenesis of strains from other serovars. We chose the isolate P125109 of the epidemic serovar Enteritidis PT4/6, generated isogenic mutants, and studied their virulence. Using in vitro and in vivo infection experiments, a dendritic cell depletion strategy, and MyD88(-/-) knockout mice, we found that P125109 employs both the "classical" and "alternative" pathways for triggering mucosal inflammation. The "classical" pathway was phenotypically similar in serovar Typhimurium strain SL1344 and in P125109. However, the kinetics of the "alternative" pathway differed significantly. Via TTSS-2, P125109 colonized the gut tissue more efficiently and triggered mucosal inflammation approximately 1 day faster than SL1344 did. In conclusion, our data demonstrate that different Salmonella spp. can differ in their capacity to trigger mucosal inflammation via the "alternative" pathway in vivo.
Suar
Mrutyunjay
M
Institute of Microbiology, D-BIOL, ETH Zürich, CH-8093 Zurich, Switzerland.
Periaswamy
Balamurugan
B
Songhet
Pascal
P
Misselwitz
Benjamin
B
Müller
Andreas
A
Käppeli
Rina
R
Kremer
Marcus
M
Heikenwalder
Mathias
M
Hardt
Wolf-Dietrich
WD
D000208:Acute Disease; D000818:Animals; D003092:Colitis / Q000276:immunology / Q000382:microbiology*; D003713:Dendritic Cells / Q000502:physiology; D006367:HeLa Cells; D006801:Humans; D051379:Mice; D008810:Mice, Inbred C57BL; D053594:Myeloid Differentiation Factor 88 / Q000502:physiology; D012480:Salmonella Infections / Q000276:immunology / Q000382:microbiology*; D012477:Salmonella enteritidis / Q000145:classification / Q000378:metabolism / Q000472:pathogenicity*; D012486:Salmonella typhimurium / Q000472:pathogenicity
C507780:Myd88 protein, mouse; D053594:Myeloid Differentiation Factor 88