Search for protein adhesin gene in Bifidobacterium longum genome using surface phage display technology.
A fragment of the nucleotide sequence encoding polypeptide binding to HT-29 epithelial cells was cloned from VMKB44 Bifidobacterium longum genome library using surface phage display technology. Sequencing of this polypeptide consisting of 26 amino acid residues showed that it is an extracellular fragment of a large BL0155 transmembrane protein belonging to the ABC transport protein superfamily. The genes encoding homologues of this protein were detected in genomes of not only bifidobacteria of different species, but also in many other enteric commencals and pathogens.
Shkoporov
A N
AN
Russian State Medical University, Federal Agency for Health care and Social Development, Russian Ministry of Health, Moscow, Russia. a.shkoporov@gmail.com
Khokhlova
E V
EV
Kafarskaia
L I
LI
Pavlov
K A
KA
Smeianov
V V
VV
Steele
J L
JL
Efimov
B A
BA
D018829:Adhesins, Bacterial / Q000737:chemistry / Q000235:genetics*; D000595:Amino Acid Sequence; D001644:Bifidobacterium / Q000235:genetics*; D016680:Genome, Bacterial / Q000235:genetics*; D019073:HT29 Cells; D006801:Humans; D019151:Peptide Library; D016415:Sequence Alignment
D018829:Adhesins, Bacterial; D019151:Peptide Library