Oral administration of curcumin emulsified in carboxymethyl cellulose has a potent anti-inflammatory effect in the IL-10 gene-deficient mouse model of IBD.
Curcumin is a tumeric-derived, water-insoluble polyphenol with potential beneficial health effects for humans. It has been shown to have preventive as well as therapeutic effects in chemically induced murine models of colitis. To investigate whether curcumin exerts a similar effect on the spontaneous colitis in interleukin (IL)-10 gene-deficient mice, we gavaged these mice daily for 2 weeks with 200 mg/kg per day curcumin emulsified in carboxymethyl cellulose, a food additive generally used as a viscosity modifier. Mice fed the curcumin/carboxymethyl cellulose mixture and those receiving carboxymethyl cellulose alone demonstrated similar reductions in histological injury score and colon weight/length ratio compared to water-fed controls. However, significant reductions in pro-inflammatory cytokine release in intestinal explant cultures were only seen in mice treated with the curcumin mixture. Our data demonstrate that in IL-10 gene-deficient mice, both oral curcumin and carboxymethyl cellulose, appear to have modifying effects on colitis. However, curcumin has additional anti-inflammatory effects mediated through a reduced production of potent pro-inflammatory mucosal cytokines.
Ung
Victoria Y L
VY
Department of Medicine, Division of Gastroenterology, Center of Excellence for Gastrointestinal Inflammation and Immunity Research, University of Alberta, Zeidler Ledcor Building, Edmonton, AB T6G 2X8, Canada.
Foshaug
Rae R
RR
MacFarlane
Sarah M
SM
Churchill
Thomas A
TA
Doyle
Jason S G
JS
Sydora
Beate C
BC
Fedorak
Richard N
RN
D000284:Administration, Oral; D000704:Analysis of Variance; D000818:Animals; D002266:Carboxymethylcellulose Sodium / Q000008:administration & dosage* / Q000494:pharmacology; D003092:Colitis / Q000235:genetics / Q000378:metabolism / Q000517:prevention & control*; D003474:Curcumin / Q000008:administration & dosage* / Q000494:pharmacology; D004195:Disease Models, Animal; D004655:Emulsions; D004797:Enzyme-Linked Immunosorbent Assay; D007371:Interferon-gamma / Q000378:metabolism; D016753:Interleukin-10 / Q000235:genetics; D020381:Interleukin-17 / Q000378:metabolism; D051379:Mice; D009195:Peroxidase / Q000378:metabolism
D004655:Emulsions; D020381:Interleukin-17; D016753:Interleukin-10; D007371:Interferon-gamma; D009195:Peroxidase; D003474:Curcumin; D002266:Carboxymethylcellulose Sodium