title: Design and synthesis of membrane fusion inhibitors against the feline immunodeficiency virus.
abstract: Feline immunodeficiency virus (FIV) is a pathogenic virus that causes an AIDS-like syndrome in the domestic cats. For viral entry and infection, fusion between the virus and the cell membrane is the critical process and this process is mediated by an envelope glycoprotein gp40. We have identified fusion inhibitory peptides from the heptad repeat-2 (HR2) of gp40. Remodeling of the original sequences using alpha-helix-inducible motifs revealed the interactive residues of gp40. Comparative analysis of HR2 peptides derived from four FIV strains demonstrated that the interactive surface of the Shizuoka strain-derived HR2 peptides provides the highest affinity of all the FIV strains examined.
authors.affiliation: Graduate School of Pharmaceutical Sciences, Kyoto University, Sakyo-ku, Kyoto 606-8501, Japan. soishi@pharm.kyoto-u.ac.jp
authors.forename: Shinya
authors.initials: S
authors.lastname: Oishi
authors.forename: Yasuyo
authors.initials: Y
authors.lastname: Kodera
authors.forename: Hiroki
authors.initials: H
authors.lastname: Nishikawa
authors.forename: Hirotaka
authors.initials: H
authors.lastname: Kamitani
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Watabe
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Ohno
authors.forename: Tadafumi
authors.initials: T
authors.lastname: Tochikura
authors.forename: Kazuki
authors.initials: K
authors.lastname: Shimane
authors.forename: Eiichi
authors.initials: E
authors.lastname: Kodama
authors.forename: Masao
authors.initials: M
authors.lastname: Matsuoka
authors.forename: Fuminori
authors.initials: F
authors.lastname: Mizukoshi
authors.forename: Hajime
authors.initials: H
authors.lastname: Tsujimoto
authors.forename: Nobutaka
authors.initials: N
authors.lastname: Fujii
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002415:Cats; D016089:Immunodeficiency Virus, Feline / Q000187:drug effects*; D008561:Membrane Fusion / Q000187:drug effects*; D008969:Molecular Sequence Data; D010455:Peptides / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology*; D011485:Protein Binding; D017433:Protein Structure, Secondary; D020449:Repetitive Sequences, Amino Acid; D013329:Structure-Activity Relationship; D014759:Viral Envelope Proteins / Q000737:chemistry / Q000378:metabolism*
chemical_list: D010455:Peptides; D014759:Viral Envelope Proteins