title: Nuclear pregnane X receptor single nucleotide polymorphism (-25385C/T) is not associated with inflammatory bowel disease in pediatric patients.
abstract: OBJECTIVE
  Studies in adults characterized the role of the pregnane X receptor (PXR) in the pathophysiology of inflammatory bowel disease (IBD) with conflicting results; pediatric studies are still lacking.
  
  
  METHODS
  Genotyping for the -25385C/T polymorphism of the PXR gene in 187 white children with IBD and 185 controls. Determination of colonic PXR expression in selected patients with IBD.
  
  
  RESULTS
  Minor allele frequency was seen in 35.6% patients with IBD and 40.5% controls (P = 0.174), although no significant differences were seen between the genotypes (P = 0.366). PXR was underexpressed in colonic tissue of 7 out of 11 Crohn disease and in 4 out of 5 patients with ulcerative colitis.
  
  
  CONCLUSIONS
  We could not confirm an association of the -25385C/T polymorphism in pediatric patients with IBD.
authors.affiliation: Department of Pediatric Surgery, Dr von Haunersches Kinderspital, Ludwig-Maximilians-University of Munich, Lindwurmstr, Munich, Germany. martin.lacher@lmu.de
authors.forename: Martin
authors.initials: M
authors.lastname: Lacher
authors.forename: Roland
authors.initials: R
authors.lastname: Kappler
authors.forename: Sebastian
authors.initials: S
authors.lastname: Schroepf
authors.forename: Stefan
authors.initials: S
authors.lastname: Berkholz
authors.forename: Antje
authors.initials: A
authors.lastname: Ballauff
authors.forename: Philip
authors.initials: P
authors.lastname: Bufler
authors.forename: Hansjoerg
authors.initials: H
authors.lastname: Baurecht
authors.forename: Dietrich
authors.initials: D
authors.lastname: von Schweinitz
authors.forename: Sibylle
authors.initials: S
authors.lastname: Koletzko
mesh_terms: D000293:Adolescent; D000328:Adult; D002648:Child; D002675:Child, Preschool; D005260:Female; D006801:Humans; D007223:Infant; D015212:Inflammatory Bowel Diseases / Q000235:genetics*; D008297:Male; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide; D000077297:Pregnane X Receptor; D012333:RNA, Messenger / Q000378:metabolism; D011987:Receptors, Steroid / Q000235:genetics*
chemical_list: D000077297:Pregnane X Receptor; D012333:RNA, Messenger; D011987:Receptors, Steroid