New approaches in the diagnosis of small-cell round tumors of bone and soft tissue.
The diagnosis of 17 small-cell round tumors of the bone and soft tissue, which were histologically similar to Ewing's sarcoma (EWS), was verified on paraffin sections, by using tissue microarray (TMA) technology, immunohistochemistry, cytogenetic (FISH) and molecular biological (RT-PCR) methods. Classical EWS was found to be in 8 patients, large-cell EWS in 1 patient; atypical EWS in 1, and endothelial ESW in 1. Two patients were diagnosed as having primitive neuroectodermal tumor (PNET), synovial sarcoma was present in 1 patient, embryonic rhabdomyosarcoma in 1, high-grade undifferentiated sarcoma in 1 and diffuse B-cell large-cell lymphoma in 1. TMA makes it possible to perform a number of diagnostic procedures on the same block containing a copious number of tumor samples and to assess the results of their use. It is emphasized that the diagnosis of small-cell round tumors requires the use of a package of the currently available methods providing the qualitative characteristics of each neoplasm.
Petrov
Semen V
SV
Department of Pathology, Kazan Cancer Center, Kazan 420029, Russia.
Machado
Isidro
I
Boulytcheva
Irena V
IV
Noguera
Rosa
R
Pellin
Antonio
A
Bacchini
Patricia
P
Bertoni
Franco
F
Llombart-Bosch
Antonio
A
D000293:Adolescent; D000328:Adult; D000368:Aged; D001859:Bone Neoplasms / Q000175:diagnosis* / Q000378:metabolism* / Q000473:pathology; D002648:Child; D002675:Child, Preschool; D005260:Female; D006801:Humans; D007223:Infant; D008297:Male; D046228:Microarray Analysis / Q000379:methods; D008875:Middle Aged; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D012512:Sarcoma, Ewing / Q000175:diagnosis / Q000378:metabolism / Q000473:pathology; D012983:Soft Tissue Neoplasms / Q000175:diagnosis* / Q000378:metabolism* / Q000473:pathology