title: Bacterial genome sequencing.
abstract: For over 30 yr, the Sanger method has been the standard for DNA sequencing. Instruments have been developed and improved over time to increase throughput, but they always relied on the same technology. Today, we are facing a revolution in DNA sequencing with many drastically different platforms that have become or will soon become available on the market. We review a number of sequencing technologies and provide examples of applications. We also discuss the impact genomics and new DNA sequencing approaches have had on various fields of biological research.
authors.affiliation: Institute for Genome Sciences, Department of Microbiology and Immunology, University of Maryland School of Medicine, Baltimore, MD, USA.
authors.forename: Hervé
authors.initials: H
authors.lastname: Tettelin
authors.forename: Tamara
authors.initials: T
authors.lastname: Feldblyum
mesh_terms: D001419:Bacteria / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification; D001424:Bacterial Infections / Q000453:epidemiology / Q000382:microbiology; D001428:Bacterial Vaccines / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification; D030561:Databases, Nucleic Acid; D020224:Expressed Sequence Tags; D014644:Genetic Variation; D016680:Genome, Bacterial; D023281:Genomics / Q000379:methods; D006801:Humans; D017720:Molecular Epidemiology / Q000379:methods*; D017422:Sequence Analysis, DNA / Q000379:methods*
chemical_list: D001428:Bacterial Vaccines; D004269:DNA, Bacterial