A llama single domain anti-idiotypic antibody mimicking HER2 as a vaccine: Immunogenicity and efficacy.
HER2 over-expression in breast cancer correlates with reduced survival. Anti-idiotypic antibodies (Abs) that closely mimic HER2 could play a crucial role in the development of effective therapeutic vaccines. Here, we show that an anti-idiotypic single domain antibody (sdAb) 1HE isolated from a library generated from a Trastuzumab F(ab')(2)-immunized llama, closely mimics HER2. SdAb 1HE shows high affinity for Trastuzumab F(ab')(2), selectively inhibits HER2 binding to Trastuzumab F(ab')(2), and sera from sdAb 1HE-immunized BALB/c mice contain anti-HER2 antibodies that inhibit viability of HER2-positive cells. These results indicate that sdAb 1HE could be used as an anti-idiotype-based vaccine to boost immunity in patients bearing HER2-positive tumours.
Alvarez-Rueda
Nidia
N
IRCM (Institut de Recherche en Cancérologie de Montpellier), INSERM U896, Université Montpellier 1, CRLC Val d'Aurelle Paul Lamarque, Montpellier Cedex 5, France.
Ladjemi
Maha Zohra
MZ
Béhar
Ghislaine
G
Corgnac
Stéphanie
S
Pugnière
Martine
M
Roquet
Françoise
F
Bascoul-Mollevi
Caroline
C
Baty
Daniel
D
Pèlegrin
André
A
Navarro-Teulon
Isabelle
I
D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000276:immunology* / Q000302:isolation & purification; D000911:Antibodies, Monoclonal / Q000276:immunology; D061067:Antibodies, Monoclonal, Humanized; D002161:Camelids, New World / Q000276:immunology*; D019496:Cancer Vaccines / Q000276:immunology*; D045744:Cell Line, Tumor; D002470:Cell Survival; D005260:Female; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D010063:Ovum / Q000276:immunology / Q000502:physiology; D019151:Peptide Library; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000037:antagonists & inhibitors*; D000068878:Trastuzumab
D000888:Antibodies, Anti-Idiotypic; D000911:Antibodies, Monoclonal; D061067:Antibodies, Monoclonal, Humanized; D019496:Cancer Vaccines; D019151:Peptide Library; D018719:Erb-b2 Receptor Tyrosine Kinases; D000068878:Trastuzumab; C524056:Erbb2 protein, mouse