title: SELDI-TOF MS profiling of serum for detection of nasopharyngeal carcinoma.
abstract: BACKGROUND
  No satisfactory biomarkers are currently available to screen for nasopharyngeal carcinoma (NPC). We have developed and evaluated surface-enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF MS) for detection and analysis of multiple proteins for distinguishing individuals with NPC from control individuals.
  
  
  METHODS
  A preliminary learning set and a classification tree of spectra derived from 24 patients with NPC and a group of 24 noncancer controls were used to develop a proteomic model that discriminated cancer from noncancer effectively. Then, the validity of the classification tree was challenged with a blind test set, which included another 20 patients with NPC and 12 noncancer controls.
  
  
  RESULTS
  A panel of 3 biomarkers ranging m/z 3-20 k was selected to establish Decision Tree model by BPS with sensitivity of 91.66% and specificity of 95.83%. The ability to detect NPC patients was evaluated, a sensitivity of 95.0% and specificity of 83.33% were validated in blind testing set.
  
  
  CONCLUSIONS
  This high-flux proteomic classification system will provide a highly accurate and innovative approach for the detection/diagnosis of NPC.
authors.affiliation: Guangxi Medical Scientific Research Center, Guangxi Medical University, Nanning, PR China. hyjgxmu@126.com
authors.forename: Yuan-Jiao
authors.initials: YJ
authors.lastname: Huang
authors.forename: Chao
authors.initials: C
authors.lastname: Xuan
authors.forename: Bei-Bei
authors.initials: BB
authors.lastname: Zhang
authors.forename: Ming
authors.initials: M
authors.lastname: Liao
authors.forename: Kai-Feng
authors.initials: KF
authors.lastname: Deng
authors.forename: Min
authors.initials: M
authors.lastname: He
authors.forename: Jin-Min
authors.initials: JM
authors.lastname: Zhao
mesh_terms: D014408:Biomarkers, Tumor / Q000097:blood*; D002294:Carcinoma, Squamous Cell / Q000175:diagnosis*; D016022:Case-Control Studies; D003663:Decision Trees; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009303:Nasopharyngeal Neoplasms / Q000175:diagnosis*; D009363:Neoplasm Proteins / Q000097:blood*; D040901:Proteomics; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
chemical_list: D014408:Biomarkers, Tumor; D009363:Neoplasm Proteins