title: Inaccuracy of single-target sequencing for discriminating species of the Mycobacterium abscessus group.
abstract: We determined nucleotide sequences of rpoB, hsp65, and sodA in 59 clinical isolates (from 58 patients) of the Mycobacterium abscessus group. Identification to the species level, based on three target genes, was concordant for 44 isolates (25 M. abscessus, 13 Mycobacterium massiliense, and 6 Mycobacterium bolletii isolates) and discordant for 15 isolates which had "interspecific composite patterns." Sequence analysis of five housekeeping genes also showed composite patterns in 8 of these 15 isolates.
authors.affiliation: Hôpital Ambroise Paré, Boulogne-Billancourt, France.
authors.forename: Edouard
authors.initials: E
authors.lastname: Macheras
authors.forename: Anne-Laure
authors.initials: AL
authors.lastname: Roux
authors.forename: Fabienne
authors.initials: F
authors.lastname: Ripoll
authors.forename: Valérie
authors.initials: V
authors.lastname: Sivadon-Tardy
authors.forename: Cristina
authors.initials: C
authors.lastname: Gutierrez
authors.forename: Jean-Louis
authors.initials: JL
authors.lastname: Gaillard
authors.forename: Beate
authors.initials: B
authors.lastname: Heym
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics; D015373:Bacterial Typing Techniques / Q000379:methods*; D018834:Chaperonin 60; D018833:Chaperonins / Q000235:genetics; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D012321:DNA-Directed RNA Polymerases / Q000235:genetics; D005838:Genotype; D006801:Humans; D008969:Molecular Sequence Data; D009161:Mycobacterium / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D009164:Mycobacterium Infections / Q000175:diagnosis* / Q000382:microbiology*; D010802:Phylogeny; D013482:Superoxide Dismutase / Q000235:genetics
chemical_list: D001426:Bacterial Proteins; D018834:Chaperonin 60; D004269:DNA, Bacterial; C074119:heat-shock protein 65, Mycobacterium; C095500:SodA protein, Bacteria; D013482:Superoxide Dismutase; D012321:DNA-Directed RNA Polymerases; C105078:RNA polymerase beta subunit; D018833:Chaperonins