In vitro synergistic effects of metergoline and antifungal agents against Candida krusei.
Metergoline, a serotonin receptor antagonist, was evaluated for its antifungal activity against the opportunistic human fungal pathogen Candida krusei by a broth microdilution assay. The minimal inhibitory concentration and minimal fungicidal concentration of metergoline against C. krusei were 4 and 8 μg ml(-1) respectively. Significant synergism was found in combination of metergoline with amphotericin B (fractional inhibitory concentration index: 0.375-0.5) by a chequerboard assay. Metergoline also inhibited extracellular phospholipase secretion in a dose-dependent manner, which may be a possible action mechanism of metergoline on C. krusei.
Kang
K
K
Department of Biochemistry (Science), The Chinese University of Hong Kong, Shatin, Hong Kong, China.
Wong
K-S
KS
Seneviratne
C Jayampath
CJ
Samaranayake
L P
LP
Fong
W-P
WP
Tsang
P W-K
PW
D000666:Amphotericin B / Q000494:pharmacology; D000935:Antifungal Agents / Q000494:pharmacology*; D002175:Candida / Q000187:drug effects*; D004357:Drug Synergism; D005656:Fungal Proteins / Q000037:antagonists & inhibitors; D008711:Metergoline / Q000494:pharmacology*; D008826:Microbial Sensitivity Tests; D050296:Microbial Viability / Q000187:drug effects; D010740:Phospholipases / Q000037:antagonists & inhibitors
D000935:Antifungal Agents; D005656:Fungal Proteins; D008711:Metergoline; D000666:Amphotericin B; D010740:Phospholipases