Relevance of the genetic polymorphism of NOD1 in Chlamydia pneumoniae seropositive stroke patients.
OBJECTIVE
Chronic infections with certain pathogens, such as Chlamydia pneumoniae, and genetic parameters that influence inflammatory reactions have been suggested to contribute to ischaemic stroke. NOD1 is a potent cytosolic receptor for C. pneumoniae. The aim of this study was to investigate the genetic polymorphism of NOD1 from the aspect of the development of stroke.


METHODS
A total of 280 patients with ischaemic stroke were enrolled in the study; 150 healthy blood donors served as controls. The G796A (E266K) NOD1 polymorphism was determined by restriction fragment length polymorphism. Chlamydia pneumoniae seropositivity was tested by ELISA.


RESULTS
There was a significant difference in NOD1 G796A genotype distribution between the controls and the stroke patients with C. pneumoniae seropositivity. The AA homozygote and GA heterozygote mutant variants were detected in 16% (25 of 152) and in 50% (77 of 152) of the C. pneumoniae-positive stroke patients, as compared with 8% (6 of 84), and 28% (24 of 84), respectively, in the C. pneumoniae-positive healthy controls. (OR = 2.559; 95% CI = 1.105-6.517, P = 0.04 and OR = 2.567; 95% CI = 1.451-4.540 P < 0.001, respectively). The stroke patients with the large vessel pathology exhibited the highest frequency of the mutant allele A (51%). In contrast, amongst the C. pneumoniae-negative subjects, no difference in genotype frequency was observed between the stroke patients and the controls.


CONCLUSIONS
Polymorphism in NOD1 G796A alone did not prove to be a risk factor for stroke in general, but in association with C. pneumoniae infection it appeared to be accompanied by an increased risk of the development of stroke.
Tiszlavicz
Z
Z
Department of Medical Microbiology and Immunobiology, University of Szeged, Szeged, Hungary.
Somogyvári
F
F
Kocsis
A K
AK
Szolnoki
Z
Z
Sztriha
L K
LK
Kis
Z
Z
Vécsei
L
L
Mándi
Y
Y
D000368:Aged; D000483:Alleles; D054458:Amplified Fragment Length Polymorphism Analysis; D016009:Chi-Square Distribution; D023521:Chlamydophila Infections / Q000150:complications* / Q000235:genetics; D016993:Chlamydophila pneumoniae / Q000235:genetics; D002908:Chronic Disease; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D008297:Male; D008875:Middle Aged; D053474:Nod1 Signaling Adaptor Protein / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide; D020521:Stroke / Q000150:complications* / Q000235:genetics
C119067:NOD1 protein, human; D053474:Nod1 Signaling Adaptor Protein