title: Transdifferentiation of human adipose tissue-derived stromal cells into insulin-producing clusters.
abstract: Type 1 diabetes mellitus is caused by autoimmune destruction of insulin-producing beta cells. The major obstacle to transplantation of insulin-producing cells to cure the disease is the limited source of these cells. To overcome this problem, we describe here a multistep protocol for generation of insulin-producing islet-like clusters from human adipose tissue-derived stromal cells (ADSCs). Analysis using reverse transcription polymerase chain reaction detected enhanced expression of various pancreatic genes during the differentiation of ADSCs. Immunofluorescence analysis revealed functional similarities between cells derived from ADSCs and pancreatic islet cells, i.e., the presence of insulin- and C-peptide-coexpressing cells in the clusters and glucagon expression on the cell surface. The glucose challenge tests revealed the production of insulin, and such production was regulated via physiological signaling pathways. Our insulin-producing cells derived from ADSCs could be potentially used for cell therapy of type 1 diabetes mellitus.
authors.affiliation: Department of Surgery, Osaka University Graduate School of Medicine, Osaka, Japan.
authors.forename: Hanayuki
authors.initials: H
authors.lastname: Okura
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Komoda
authors.forename: Yuichi
authors.initials: Y
authors.lastname: Fumimoto
authors.forename: Chun-Man
authors.initials: CM
authors.lastname: Lee
authors.forename: Toshirou
authors.initials: T
authors.lastname: Nishida
authors.forename: Yoshiki
authors.initials: Y
authors.lastname: Sawa
authors.forename: Akifumi
authors.initials: A
authors.lastname: Matsuyama
mesh_terms: D000273:Adipose Tissue / Q000166:cytology*; D000328:Adult; D054338:Cell Transdifferentiation; D005260:Female; D015870:Gene Expression; D006801:Humans; D007328:Insulin / Q000378:metabolism; D000078790:Insulin Secretion; D050417:Insulin-Secreting Cells / Q000166:cytology* / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D010187:Pancreatic Hormones / Q000378:metabolism; D017154:Stromal Cells
chemical_list: D007328:Insulin; D010187:Pancreatic Hormones