A novel type of dual-modality molecular probe for MR and nuclear imaging of tumor: preparation, characterization and in vivo application.
A novel dual-modality molecular probe composed of biocompatible Fe(3)O(4) nanocrystal, monoclonal antibody and radionuclide was designed and prepared. All functional components in the dual-modality molecular probe, i.e., Fe(3)O(4), PEG, mAb 3H11 and (125)I, were chemically bonded together for forming a stable molecular probe. Systematic in vitro experiments were carried out for evaluating the biological activity of the antibody in the targeting probe. A series of in vivo experiments were performed based on the dual-modality imaging probe for detecting xenografted tumors in nude mice by MRI and gamma-imaging techniques. The pharmacokinetics of the dual-modality molecular probe in tumor-bearing nude mice was studied.
Liu
Shujie
S
Institute of Chemistry, CAS, Bei Yi Jie 2, Beijing 100190, China.
Jia
Bing
B
Qiao
Ruirui
R
Yang
Zhi
Z
Yu
Zilin
Z
Liu
Zhaofei
Z
Liu
Kan
K
Shi
Jiyun
J
Ouyang
Han
H
Wang
Fan
F
Gao
Mingyuan
M
D000818:Animals; D000911:Antibodies, Monoclonal / Q000737:chemistry / Q000493:pharmacokinetics*; D003952:Diagnostic Imaging; D005260:Female; D052203:Ferrosoferric Oxide / Q000737:chemistry*; D006801:Humans; D007457:Iodine Radioisotopes / Q000737:chemistry / Q000493:pharmacokinetics*; D008279:Magnetic Resonance Imaging; D053768:Metal Nanoparticles; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D015335:Molecular Probes / Q000737:chemistry / Q000493:pharmacokinetics*; D009374:Neoplasms, Experimental / Q000175:diagnosis*; D049268:Positron-Emission Tomography; D019275:Radiopharmaceuticals; D014018:Tissue Distribution; D023041:Xenograft Model Antitumor Assays
D000911:Antibodies, Monoclonal; D007457:Iodine Radioisotopes; D015335:Molecular Probes; D019275:Radiopharmaceuticals; D052203:Ferrosoferric Oxide