title: The fatty acid synthase of the basidiomycete Omphalotus olearius is a single polypeptide.
abstract: Fatty acids are essential components of almost all biological membranes. Additionally, they are important in energy storage, as second messengers during signal transduction, and in post-translational protein modification. De novo synthesis of fatty acids is essential for almost all organisms, and entails the iterative elongation of the growing fatty acid chain through a set of reactions conserved in all kingdoms. During our work on the biosynthesis of secondary metabolites, a 450-kDa protein was detected by SDS-PAGE of enriched fractions from mycelial lysates from the basidiomycete Omphalotus olearius. Protein sequencing of this protein band revealed the presence of peptides with homology to both alpha and beta subunits of the ascomycete fatty acid synthase (FAS) family. The FAS encoding gene of O. olearius was sequenced. The positions of its predicted 21 introns were verified. The gene encodes a 3931 amino acids single protein, with an equivalent of the ascomycetous beta subunit at the N-terminus and the a subunit at the C-terminus. This is the first report on an FAS protein from a homobasidiomycete and also the first fungal FAS which is comprised of a single polypeptide.
authors.affiliation: IBWF, Institut für Biotechnologie und Wirkstoff-Forschung e. V., Erwin-Schrödinger-Str. 56, D-67663 Kaiserslautern, Germany. antelo@ibwf.de
authors.forename: Luis
authors.initials: L
authors.lastname: Antelo
authors.forename: Angela
authors.initials: A
authors.lastname: Schlipp
authors.forename: Carolin
authors.initials: C
authors.lastname: Hof
authors.forename: Katrin
authors.initials: K
authors.lastname: Eisfeld
authors.forename: Holger
authors.initials: H
authors.lastname: Berg
authors.forename: Till
authors.initials: T
authors.lastname: Hornbogen
authors.forename: Rainer
authors.initials: R
authors.lastname: Zocher
authors.forename: Heidrun
authors.initials: H
authors.lastname: Anke
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001487:Basidiomycota / Q000201:enzymology*; D003455:Cryptococcus neoformans / Q000201:enzymology; D064429:Fatty Acid Synthases / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D005656:Fungal Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D008322:Mammals; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010455:Peptides / Q000737:chemistry
chemical_list: D005656:Fungal Proteins; D010455:Peptides; D064429:Fatty Acid Synthases