Identification of the plasticity-relevant fucose-alpha(1-2)-galactose proteome from the mouse olfactory bulb.
Fucose-alpha(1-2)-galactose [Fucalpha(1-2)Gal] sugars have been implicated in the molecular mechanisms that underlie neuronal development, learning, and memory. However, an understanding of their precise roles has been hampered by a lack of information regarding Fucalpha(1-2)Gal glycoproteins. Here, we report the first proteomic studies of this plasticity-relevant epitope. We identify five classes of putative Fucalpha(1-2)Gal glycoproteins: cell adhesion molecules, ion channels and solute carriers/transporters, ATP-binding proteins, synaptic vesicle-associated proteins, and mitochondrial proteins. In addition, we show that Fucalpha(1-2)Gal glycoproteins are enriched in the developing mouse olfactory bulb (OB) and exhibit a distinct spatiotemporal expression that is consistent with the presence of a "glycocode" to help direct olfactory sensory neuron (OSN) axonal pathfinding. We find that expression of Fucalpha(1-2)Gal sugars in the OB is regulated by the alpha(1-2)fucosyltransferase FUT1. FUT1-deficient mice exhibit developmental defects, including fewer and smaller glomeruli and a thinner olfactory nerve layer, suggesting that fucosylation contributes to OB development. Our findings significantly expand the number of Fucalpha(1-2)Gal glycoproteins and provide new insights into the molecular mechanisms by which fucosyl sugars contribute to neuronal processes.
Murrey
Heather E
HE
Howard Hughes Medical Institute and Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, California 91125, USA.
Ficarro
Scott B
SB
Krishnamurthy
Chithra
C
Domino
Steven E
SE
Peters
Eric C
EC
Hsieh-Wilson
Linda C
LC
D000818:Animals; D001921:Brain / Q000033:anatomy & histology / Q000378:metabolism; D002846:Chromatography, Affinity; D004187:Disaccharides / Q000032:analysis*; D000939:Epitopes; D005647:Fucosyltransferases / Q000235:genetics / Q000378:metabolism; D007527:Isoenzymes / Q000235:genetics / Q000378:metabolism; D037102:Lectins / Q000378:metabolism; D008297:Male; D013058:Mass Spectrometry; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D009473:Neuronal Plasticity / Q000502:physiology*; D009830:Olfactory Bulb / Q000737:chemistry / Q000166:cytology / Q000502:physiology; D020543:Proteome; D051381:Rats; D014018:Tissue Distribution; D000097631:Galactoside 2-alpha-L-fucosyltransferase
D004187:Disaccharides; D000939:Epitopes; D005647:Fucosyltransferases; D007527:Isoenzymes; D037102:Lectins; D020543:Proteome; D000097631:Galactoside 2-alpha-L-fucosyltransferase; C112685:fucosyl-1-2-galactose