Syntaxin specificity of aquaporins in the inner medullary collecting duct.
Proper targeting of the aquaporin-2 (AQP2) water channel to the collecting duct apical plasma membrane is critical for the urine concentrating mechanism and body water homeostasis. However, the trafficking mechanisms that recruit AQP2 to the plasma membrane are still unclear. Snapin is emerging as an important mediator in the initial interaction of trafficked proteins with target soluble N-ethylmaleimide-sensitive factor attachment protein (SNAP) receptor (t-SNARE) proteins, and this interaction is functionally important for AQP2 regulation. We show that in AQP2-Madin-Darby canine kidney cells subjected to adenoviral-mediated expression of both snapin and syntaxins, the association of AQP2 with both syntaxin-3 and syntaxin-4 is highly enhanced by the presence of snapin. In pull-down studies, snapin detected AQP2, syntaxin-3, syntaxin-4, and SNAP23 from the inner medullary collecting duct. AQP2 transport activity, as probed by AQP2's urea permeability, was greatly enhanced in oocytes that were coinjected with cRNAs of SNARE components (snapin+syntaxin-3+SNAP23) over those injected with AQP2 cRNA alone. It was not enhanced when syntaxin-3 was replaced by syntaxin-4 (snapin+syntaxin-4+SNAP23). On the other hand, the latter combination significantly enhanced the transport activity of the related AQP3 water channel while the presence of syntaxin-3 did not. This AQP-syntaxin interaction agrees with the polarity of these proteins' expression in the inner medullary collecting duct epithelium. Thus our findings suggest a selectivity of interactions between different aquaporin and syntaxin isoforms, and thus in the regulation of AQP2 and AQP3 activities in the plasma membrane. Snapin plays an important role as a linker between the water channel and the t-SNARE complex, leading to the fusion event, and the pairing with specific t-SNAREs is essential for the specificity of membrane recognition and fusion.
Mistry
Abinash C
AC
Department of Physiology, Emory University School of Medicine, Atlanta, Georgia 30322, USA.
Mallick
Rickta
R
Klein
Janet D
JD
Weimbs
Thomas
T
Sands
Jeff M
JM
Fröhlich
Otto
O
D000818:Animals; D051399:Aquaporin 2 / Q000235:genetics / Q000378:metabolism*; D051400:Aquaporin 3 / Q000235:genetics / Q000378:metabolism*; D001692:Biological Transport; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism*; D002463:Cell Membrane Permeability; D000242:Cyclic AMP / Q000378:metabolism; D017868:Cyclic AMP-Dependent Protein Kinases / Q000378:metabolism; D004285:Dogs; D005260:Female; D006801:Humans; D007685:Kidney Tubules, Collecting / Q000378:metabolism*; D007700:Kinetics; D008297:Male; D026901:Membrane Transport Proteins / Q000378:metabolism; D009154:Mutation; D009865:Oocytes; D011485:Protein Binding; D021381:Protein Transport; D050765:Qa-SNARE Proteins / Q000235:genetics / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D014162:Transfection; D014508:Urea / Q000378:metabolism; D050988:Vesicle-Associated Membrane Protein 2 / Q000378:metabolism; D033921:Vesicular Transport Proteins / Q000378:metabolism; D014882:Water-Electrolyte Balance; D014982:Xenopus laevis
C496025:Aqp2 protein, rat; C496029:Aqp3 protein, rat; D051399:Aquaporin 2; D026901:Membrane Transport Proteins; D050765:Qa-SNARE Proteins; D011993:Recombinant Fusion Proteins; C118519:SNAPIN protein, human; C494706:Vamp2 protein, rat; D050988:Vesicle-Associated Membrane Protein 2; D033921:Vesicular Transport Proteins; D051400:Aquaporin 3; D014508:Urea; D000242:Cyclic AMP; D017868:Cyclic AMP-Dependent Protein Kinases