Amino acid precursors for the detection of transketolase activity in Escherichia coli auxotrophs.
Probes were developed for the in vivo detection of transketolase activity by the use of a complementation assay in Escherichia coli auxotrophs They combine the d-threo ketose moiety recognised by transketolase and the side chain of leucine or methionine. These compounds were donor substrates of yeast transketolase leading to the release of the corresponding alpha-hydroxyaldehydes which could be converted in E. coli by a cascade of reactions into leucine or methionine required for cellular growth.
Simon
Grégory
G
Clermont Université, Université Blaise Pascal, Laboratoire SEESIB, UMR 6504 CNRS, 63177 Aubière Cedex, France.
Bouzon
Madeleine
M
Charmantray
Franck
F
Hélaine
Virgil
V
Légeret
Bertrand
B
Marlière
Philippe
P
Hecquet
Laurence
L
D000447:Aldehydes / Q000737:chemistry*; D004926:Escherichia coli / Q000201:enzymology* / Q000254:growth & development; D006588:Hexanones / Q000737:chemistry*; D007930:Leucine / Q000096:biosynthesis; D008715:Methionine / Q000096:biosynthesis; D009154:Mutation; D013440:Sulfides / Q000737:chemistry*; D014174:Transketolase / Q000737:chemistry / Q000378:metabolism*
D000447:Aldehydes; D006588:Hexanones; D013440:Sulfides; D008715:Methionine; D014174:Transketolase; D007930:Leucine