The efficiency of a novel delivery system (PEI600-Tat) in development of potent DNA vaccine using HPV16 E7 as a model antigen.
DNA vaccination is a promising approach for inducing both humoral and cellular immune responses. The mode of plasmid DNA delivery is critical to make progress in DNA vaccination. Using human papillomavirus type 16 E7 as a model antigen, this study evaluated the effect of peptide-polymer hybrid including PEI600-Tat conjugate as a novel gene delivery system on the potency of antigen-specific immunity in mice model. At ratio of 10:50 PEI-Tat/E7DNA (w/w), both humoral and cellular immune responses were significantly enhanced as compared with E7DNA construct and induced Th1 response. Therefore, this new delivery system could have promising applications in gene therapy.
Bolhassani
Azam
A
Pasteur Institute of Iran, Tehran, Iran.
Ghasemi
Nahid
N
Servis
Catherine
C
Taghikhani
Mohammad
M
Rafati
Sima
S
D000818:Animals; D000917:Antibody Formation / Q000187:drug effects; D001360:Avidin / Q000737:chemistry; D004337:Drug Carriers / Q000008:administration & dosage / Q000737:chemistry; D016503:Drug Delivery Systems; D005260:Female; D006801:Humans; D007111:Immunity, Cellular / Q000187:drug effects; D051379:Mice; D008810:Mice, Inbred C57BL; D009856:Oncogene Proteins, Viral / Q000235:genetics* / Q000276:immunology; D050725:Papillomavirus E7 Proteins; D011094:Polyethyleneimine / Q000737:chemistry; D014162:Transfection; D019444:Vaccines, DNA / Q000008:administration & dosage* / Q000737:chemistry / Q000276:immunology
D004337:Drug Carriers; D009856:Oncogene Proteins, Viral; C528510:PEI600-avidin; D050725:Papillomavirus E7 Proteins; D019444:Vaccines, DNA; C059731:oncogene protein E7, Human papillomavirus type 16; D001360:Avidin; D011094:Polyethyleneimine