Intracerebral Mycobacterium bovis bacilli Calmette-Guerin infection-induced immune responses in the CNS.
To study whether cerebral mycobacterial infection induces granuloma and protective immunity similar to systemic infection, we intracerebrally infected mice with Mycobacterium bovis bacilli Calmette-Guerin. Granuloma and IFN-gamma(+)CD4(+) T cell responses are induced in the central nervous system (CNS) similar to periphery, but the presence of IFN-gammaIL-17 double-positive CD4(+) T cells is unique to the CNS. The major CNS source of TNF-alpha is microglia, with modest production by CD4(+) T cells and macrophage. Protective immunity is accompanied by accumulation of Foxp3(+)CD4(+) T cells and PD-L2(+) dendritic cells, suggesting that both inflammatory and anti-inflammatory responses develop in the CNS following mycobacterial infection.
Lee
JangEun
J
Department of Pathology and Laboratory Medicine, University of Wisconsin, School of Medicine and Public Health, 1300 University Avenue, Madison, WI 53706, USA.
Ling
Changying
C
Kosmalski
Michelle M
MM
Hulseberg
Paul
P
Schreiber
Heidi A
HA
Sandor
Matyas
M
Fabry
Zsuzsanna
Z
D000818:Animals; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology / Q000378:metabolism; D002490:Central Nervous System / Q000276:immunology* / Q000382:microbiology / Q000503:physiopathology; D002540:Cerebral Cortex / Q000276:immunology / Q000382:microbiology / Q000503:physiopathology; D003713:Dendritic Cells / Q000276:immunology / Q000378:metabolism; D004195:Disease Models, Animal; D004660:Encephalitis / Q000276:immunology* / Q000382:microbiology / Q000503:physiopathology; D005260:Female; D051858:Forkhead Transcription Factors / Q000378:metabolism; D006099:Granuloma / Q000276:immunology* / Q000382:microbiology / Q000503:physiopathology; D007371:Interferon-gamma / Q000378:metabolism; D020381:Interleukin-17 / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D009163:Mycobacterium bovis / Q000276:immunology*; D010455:Peptides / Q000378:metabolism; D061027:Programmed Cell Death 1 Ligand 2 Protein; D014376:Tuberculosis / Q000276:immunology* / Q000662:veterinary*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
D051858:Forkhead Transcription Factors; C498833:Foxp3 protein, mouse; D020381:Interleukin-17; C556978:Pdcd1lg2 protein, mouse; D010455:Peptides; D061027:Programmed Cell Death 1 Ligand 2 Protein; D014409:Tumor Necrosis Factor-alpha; D007371:Interferon-gamma