title: Production of heterologous proteins using the fission-yeast (Schizosaccharomyces pombe) expression system.
abstract: The fission yeast Schizosaccharomyces pombe is a particularly useful model for studying the function and regulation of genes from higher eukaryotes. The genome of Sc. pombe has been sequenced, and DNA microarray, proteome and transcriptome analyses have been carried out. Among the well-characterized yeast species, Sc. pombe is considered an attractive host for the production of heterologous proteins. Expression vectors for high-level expression in Sc. pombe have been developed and many foreign proteins have been successfully expressed. However, further improvements in the protein-expressing host systems are still required for the production of heterologous proteins involved in post-translational modification, metabolism and intracellular trafficking. This minireview focuses on recent advances in heterologous protein production by use of engineered fission-yeast strains.
authors.affiliation: Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University, Hakozaki 6-10-1, Fukuoka 812-8581, Japan. takegawa@agr.kyushu-u.ac.jp
authors.forename: Kaoru
authors.initials: K
authors.lastname: Takegawa
authors.forename: Hideki
authors.initials: H
authors.lastname: Tohda
authors.forename: Mayumi
authors.initials: M
authors.lastname: Sasaki
authors.forename: Alimjan
authors.initials: A
authors.lastname: Idiris
authors.forename: Takao
authors.initials: T
authors.lastname: Ohashi
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Mukaiyama
authors.forename: Yuko
authors.initials: Y
authors.lastname: Giga-Hama
authors.forename: Hiromichi
authors.initials: H
authors.lastname: Kumagai
mesh_terms: D005818:Genetic Engineering; D005822:Genetic Vectors / Q000235:genetics; D020411:Oligonucleotide Array Sequence Analysis; D016133:Polymerase Chain Reaction; D011994:Recombinant Proteins / Q000096:biosynthesis* / Q000235:genetics*; D012568:Schizosaccharomyces / Q000235:genetics* / Q000378:metabolism
chemical_list: D011994:Recombinant Proteins