[The polymorphism of ten STR loci in Chinese Han population in Chengdu].
OBJECTIVE
To obtain data of polymorphism distribution of 10 short tandem repeat (STR) D1S2145, D3S2433, D5S1507, D5S2502, D8S2319, D9S926, D16S767, D17S2181, GATA140E03, GATA196B10 in Chinese Han population in Chengdu and to evaluate their usefulness in the field of forensic science and their species specificity.


METHODS
DNA of 100 unrelated individuals of Chengdu Han population was extracted with Chelex method, amplified by PCR, then typed with silver staining after polyacrylamide gel electrophoresis(PAGE). Ten different animals were selected as the controls in this study for evaluating the species specificity of the ten STR loci.


RESULTS
In the ten STR loci of Chengdu Han population, 6, 5, 8, 5, 6, 7, 7, 5, 7 and 7 alleles were found, respectively. 17, 14, 28, 15, 16, 18, 15, 14, 19 and 21 genotypes were observed in the ten loci, respectively. The allele and genotype frequency distributions of the ten loci were detected no deviation from the Hardy-Weinberg law of equilibrium. By comparison with the data from 10 different animals, the species specificity of D3S2433, D5S1507, D5S2502, D8S2319 and GATA196B10 was good, but part of animals had amplification product at typing field of the other loci.


CONCLUSIONS
The 10 STR loci mentioned above are highly polymorphic and can be used in the forensic personal identification and paternity testing.
Luo
Hao
H
Department of Forensic Biology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China.
Yan
Jing
J
Zhang
Lin
L
Liao
Miao
M
Li
Ying-Bi
YB
Hou
Yi-Ping
YP
Wu
Jin
J
D000483:Alleles; D000818:Animals; D044466:Asian People / Q000235:genetics; D002681:China / Q000208:ethnology; D004247:DNA / Q000235:genetics; D005260:Female; D053803:Forensic Genetics / Q000379:methods*; D005838:Genotype; D006801:Humans; D008297:Male; D018895:Microsatellite Repeats / Q000235:genetics*; D010334:Paternity; D011110:Polymorphism, Genetic; D013045:Species Specificity
D004247:DNA