Mouse adenovirus type 1 infection of macrophages.
Mouse adenovirus type 1 (MAV-1) causes acute and persistent infections in mice, with high levels of virus found in the brain, spinal cord and spleen in acute infections. MAV-1 infects endothelial cells throughout the mouse, and monocytes/macrophages have also been implicated as targets of the virus. Here we determined the extent and functional importance of macrophage infection by MAV-1. Bone marrow-derived macrophages expressed MAV-1 mRNAs and proteins upon ex vivo infection. Adherent peritoneal macrophages from infected mice expressed viral mRNAs and produced infectious virus. Infected chemokine (C-C motif) receptor 2 (CCR2) knockout mice, which are defective for macrophage recruitment, did not show differences in survival or MAV-1 load compared to controls. In contrast, macrophage depletion using clodronate-loaded liposomes resulted in increased virus replication in spleens of a MAV-1-resistant mouse strain, BALB/cJ. Thus macrophages serve both as targets of infection and as effectors of the host response.
Ashley
Shanna L
SL
Department of Microbiology and Immunology, University of Michigan, Ann Arbor, MI 48109, USA.
Welton
Amanda R
AR
Harwood
Kirsten M
KM
Van Rooijen
Nico
N
Spindler
Katherine R
KR
D000256:Adenoviridae / Q000276:immunology / Q000472:pathogenicity*; D000257:Adenoviridae Infections / Q000276:immunology / Q000662:veterinary* / Q000821:virology; D000818:Animals; D008264:Macrophages / Q000276:immunology / Q000821:virology*; D017737:Macrophages, Peritoneal / Q000382:microbiology / Q000821:virology*; D051379:Mice; D008807:Mice, Inbred BALB C; D018345:Mice, Knockout; D012333:RNA, Messenger / Q000096:biosynthesis; D012367:RNA, Viral / Q000096:biosynthesis; D054390:Receptors, CCR2 / Q000172:deficiency; D013154:Spleen / Q000821:virology; D016019:Survival Analysis; D014764:Viral Proteins / Q000096:biosynthesis
C515335:Ccr2 protein, mouse; D012333:RNA, Messenger; D012367:RNA, Viral; D054390:Receptors, CCR2; D014764:Viral Proteins