title: Occurrence and genotypes of Giardia isolated from lambs in Spain.
abstract: Three hundred and eighty six faecal specimens were randomly collected from 1- to 3-month-old lambs from 16 farms in Spain to investigate the presence of different genotypes of Giardia duodenalis. Individual specimens were examined by IFA (Immunofluorescence assay) and beta-giardin PCR polymerase chain reaction. Cysts of G. duodenalis were shed by lambs in every flock analyzed, showing a prevalence by farms of 100%. The average prevalence of G. duodenalis for the 386 specimens was 42%, ranging from 8.3 to 80% depending on the farm. beta-giardin PCR positive samples were sequenced to determine the genotypes present at each farm and seven new subtypes of beta-giardin Assemblage E are reported in this study. In each farm, one to six different beta-giardin subtypes were found, showing the high variability of the target. Also, one flock had the zoonotic Assemblage A. This is the first report of Giardia subgenotype A-1 in sheep in Spain.
authors.affiliation: Departamento de Producción Animal, Sanidad Animal y Ciencia y Tecnología de los Alimentos, Facultad de Veterinaria, Universidad CEU Cardenal Herrera, Avda. Seminario S/N 46113 Moncada, Valencia, Spain. mtgomez@uch.ceu.es
authors.forename: María Teresa
authors.initials: MT
authors.lastname: Gómez-Muñoz
authors.forename: Cristina
authors.initials: C
authors.lastname: Navarro
authors.forename: María Magdalena
authors.initials: MM
authors.lastname: Garijo-Toledo
authors.forename: María Auxiliadora
authors.initials: MA
authors.lastname: Dea-Ayuela
authors.forename: Salceda
authors.initials: S
authors.lastname: Fernández-Barredo
authors.forename: María Teresa
authors.initials: MT
authors.lastname: Pérez-Gracia
authors.forename: María Victoria
authors.initials: MV
authors.lastname: Domínguez-Márquez
authors.forename: Rafael
authors.initials: R
authors.lastname: Borrás
mesh_terms: D000818:Animals; D003598:Cytoskeletal Proteins / Q000235:genetics; D005243:Feces / Q000469:parasitology; D005455:Fluorescent Antibody Technique / Q000379:methods; D005838:Genotype; D005872:Giardia / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D005873:Giardiasis / Q000453:epidemiology / Q000469:parasitology / Q000662:veterinary*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000662:veterinary; D015995:Prevalence; D015800:Protozoan Proteins / Q000235:genetics; D017422:Sequence Analysis, DNA; D012756:Sheep / Q000469:parasitology*; D012757:Sheep Diseases / Q000453:epidemiology* / Q000469:parasitology; D013030:Spain / Q000453:epidemiology; D015047:Zoonoses / Q000453:epidemiology / Q000469:parasitology
chemical_list: D003598:Cytoskeletal Proteins; D015800:Protozoan Proteins; C038207:giardin protein, Giardia lamblia