Inhibition of uropathogens by lactic acid bacteria isolated from dairy foods and cow's intestine in western Nigeria.
A total of 96 lactic acid bacteria (LAB) were isolated from African indigenous fermented products and cow's intestines to study their inhibitory capability against multi-drug-resistant uropathogens. Escherichia coli accounted for approximately 45% of isolated uropathogens, followed by Staphylococcus spp. (20%). The Gram negative uropathogens were highly resistant to quinolones, co-trimoxazole, teicoplanin and some beta-lactams, while the Staphylococcus spp. showed high resistance to aminoglycosides, beta-lactams and macrolides. Twenty-four LAB isolates were selected based on their antimicrobial activity against two uropathogenic Staphylococcus aureus strains and bacteriocin production. LAB strains showing antimicrobial activity were grouped into smaller groups through amplified ribosomal DNA restriction analysis (ARDRA). Representative strains were identified as Weissella spp., Enterococcus faecium, Lactococcus lactis and Lactobacillus brevis through sequencing of 16S rDNA. The Weissella spp. and L. brevis strains demonstrated remarkable inhibitory activity against seven strains of Gram negative uropathogens. Two strains of L. lactis produced a bacteriocin-like inhibitory substance active against Lactobacillus sakei. In this study, an unusual high rate of co-trimoxazole, quinolones and macrolides resistance among uropathogens from south west Nigeria was discovered. Based on their sensitivity to Weissella spp., there is a potential for using these LAB as a natural approach for the protection against the uropathogens assayed.
Ayeni
Funmilola A
FA
Department of Pharmaceutical Microbiology, University of Ibadan, Ibadan, Nigeria. funmiyeni@yahoo.co.uk
Adeniyi
Bolanle A
BA
Ogunbanwo
Samuel T
ST
Tabasco
Raquel
R
Paarup
Torsten
T
Peláez
Carmen
C
Requena
Teresa
T
D000818:Animals; D000898:Antibiosis; D001430:Bacteriocins / Q000096:biosynthesis; D002417:Cattle / Q000382:microbiology*; D004269:DNA, Bacterial / Q000235:genetics; D003611:Dairy Products / Q000382:microbiology*; D024881:Drug Resistance, Bacterial; D004926:Escherichia coli / Q000235:genetics / Q000302:isolation & purification / Q000472:pathogenicity; D005516:Food Microbiology; D006801:Humans; D007422:Intestines / Q000382:microbiology*; D007778:Lactobacillus / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism / Q000502:physiology; D013294:Lactococcus lactis / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism / Q000502:physiology; D009549:Nigeria; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013211:Staphylococcus aureus / Q000235:genetics / Q000302:isolation & purification / Q000472:pathogenicity*; D014552:Urinary Tract Infections / Q000382:microbiology
D001430:Bacteriocins; D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S