title: Functional equivalence of the clathrin heavy chains CHC17 and CHC22 in endocytosis and mitosis.
abstract: Clathrin is crucial for endocytosis and plays a recently described role in mitosis. Two clathrin heavy chains (CHCs) are found in humans: the ubiquitous CHC17, and CHC22, a CHC that is enriched in skeletal muscle. Functional differences have been proposed for these clathrins despite high sequence similarity. Here, we compared each paralogue in functional assays of endocytosis and mitosis. We find that CHC17 and CHC22 are functionally equivalent. We also describe how previous work on CHC22 has involved a splice variant that is not usually expressed in cells.
authors.affiliation: The Physiological Laboratory, School of Biomedical Sciences, University of Liverpool, Liverpool L69 3BX, UK.
authors.forename: Fiona E
authors.initials: FE
authors.lastname: Hood
authors.forename: Stephen J
authors.initials: SJ
authors.lastname: Royle
mesh_terms: D002460:Cell Line; D002966:Clathrin / Q000235:genetics / Q000378:metabolism / Q000648:ultrastructure; D033922:Clathrin Heavy Chains / Q000235:genetics / Q000378:metabolism* / Q000648:ultrastructure; D004705:Endocytosis / Q000502:physiology*; D006801:Humans; D008938:Mitosis / Q000502:physiology*; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism* / Q000648:ultrastructure; D034622:RNA Interference; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: C543704:CLTC protein, human; C101272:CLTCL1 protein, human; D002966:Clathrin; D020033:Protein Isoforms; D011993:Recombinant Fusion Proteins; D033922:Clathrin Heavy Chains