title: Anti-inflammatory activity of Motherwort (Leonurus sibiricus L.).
abstract: Motherwort (MW), a Korean folk medicine, has been applied to treat inflammatory disease. However, its effect on inflammatory cytokine release from mast cells is not well known. We investigated the anti- inflammatory effect of MW on the secretion of inflammatory cytokine such as tumor necrosis factor (TNF)-alpha and interleukin (IL)-6 and IL-8 in human mast cell line (HMC-1). MW was treated in vitro before activation of HMC-1 cells with phorbol 12-myristate 13-acetate (PMA) plus calcium ionophore A23187. MW had no cytotoxic effects on HMC-1 cell viability. MW (1 mg/ml) inhibited PMA plus A23187-stimulated gene expression and production of TNF-alpha, IL-6, and IL-8. Stimulation with PMA plus A23187 induced NF-kappaB activation in HMC-1 cells, which was inhibited by MW (1 mg/ml). MW inhibited secretion of TNF-alpha, IL-6, and IL-8 possibly by inhibiting NF-kappaB activation. These results indicate that MW may be helpful in regulating inflammatory diseases.
authors.affiliation: College of Pharmacy, Woosuk University, Jeonbuk, South Korea.
authors.forename: Hye-Young
authors.initials: HY
authors.lastname: Shin
authors.forename: Sang-Hyun
authors.initials: SH
authors.lastname: Kim
authors.forename: Sin-Myoung
authors.initials: SM
authors.lastname: Kang
authors.forename: Ik-Jun
authors.initials: IJ
authors.lastname: Chang
authors.forename: Sang-Yong
authors.initials: SY
authors.lastname: Kim
authors.forename: Hoon
authors.initials: H
authors.lastname: Jeon
authors.forename: Kang-Hyun
authors.initials: KH
authors.lastname: Leem
authors.forename: Won-Hwan
authors.initials: WH
authors.lastname: Park
authors.forename: Jong-Pil
authors.initials: JP
authors.lastname: Lim
authors.forename: Tae-Yong
authors.initials: TY
authors.lastname: Shin
mesh_terms: D000893:Anti-Inflammatory Agents / Q000378:metabolism / Q000494:pharmacology*; D000001:Calcimycin / Q000494:pharmacology; D006801:Humans; D007249:Inflammation / Q000188:drug therapy; D015850:Interleukin-6 / Q000378:metabolism; D016209:Interleukin-8 / Q000378:metabolism; D031332:Leonurus / Q000378:metabolism*; D008407:Mast Cells / Q000187:drug effects* / Q000276:immunology; D008519:Medicine, Traditional; D016328:NF-kappa B / Q000378:metabolism; D008517:Phytotherapy; D010936:Plant Extracts / Q000378:metabolism / Q000494:pharmacology*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D000893:Anti-Inflammatory Agents; D015850:Interleukin-6; D016209:Interleukin-8; D016328:NF-kappa B; D010936:Plant Extracts; D014409:Tumor Necrosis Factor-alpha; D000001:Calcimycin; D013755:Tetradecanoylphorbol Acetate