title: [Effects of different proportions of cryoprotectant to seminal plasma on the motility of post-thaw human sperm].
abstract: OBJECTIVE
  To investigate the effects of different proportions of cryoprotectant to seminal plasma on the motility of post-thaw human sperm.
  
  
  METHODS
  Different proportions of cryoprotectant to seminal plasma (1:1 and 1:3) were used for freezing sperm, and the forward movement and total motility rates of the frozen-thawed sperm were compared.
  
  
  RESULTS
  The forward movement and total motility rates were (58.60 +/- 5.57)% and (66.17 +/- 5.24)% before cryopreservation. The 1:1 proportion achieved post-thaw forward movement and total motility rates of (40.53 +/- 8.97)% and (51.23 +/- 9. 30)%, while the 1:3 (44.7 +/- 8.67)% and (51.50 +/- 7.40)%, respectively. Significantly decreased sperm motility was observed after cryopreservation (P < 0.05). Statistically significant differences were found in the forward movement but not in the total motility of the frozen-thawed sperm between the two proportions.
  
  
  CONCLUSIONS
  Cryopreservation causes obvious damage to human sperm. Higher proportion of cryoprotectant to seminal plasma (1:3) can improve the forward movement of post-thaw sperm as compared with the lower one (1:1).
authors.affiliation: Center for Reproductive Medicine, Shandong Provincial Hospital, Shandong University, Jinan, Shandong 250021, China. jingmei_hu8@hotmail.com
authors.forename: Jing-Mei
authors.initials: JM
authors.lastname: Hu
authors.forename: Cheng-Yan
authors.initials: CY
authors.lastname: Xu
authors.forename: Yuan
authors.initials: Y
authors.lastname: Li
authors.forename: Ling
authors.initials: L
authors.lastname: Geng
authors.forename: Gang
authors.initials: G
authors.lastname: Ma
authors.forename: Li
authors.initials: L
authors.lastname: Wang
authors.forename: Zi-Jiang
authors.initials: ZJ
authors.lastname: Chen
mesh_terms: D000328:Adult; D003451:Cryoprotective Agents / Q000009:adverse effects / Q000494:pharmacology*; D005615:Freezing; D006801:Humans; D008297:Male; D012661:Semen / Q000187:drug effects*; D013081:Sperm Motility / Q000187:drug effects*
chemical_list: D003451:Cryoprotective Agents