title: Plasmonic nanoprobes for SERS biosensing and bioimaging.
abstract: This article provides an overview of the development and application of plasmonic nanoprobes developed in our laboratory for biosensing and bioimaging. We describe the use of plasmonics surface-enhanced Raman scattering (SERS) gene probes for the detection of diseases using DNA hybridization to target biospecies (HIV gene, breast cancer genes etc.). For molecular imaging, we describe a hyperspectral surface-enhanced Raman imaging (HSERI) system that combines imaging capabilities with SERS detection to identify cellular components using Raman dye-labeled silver nanoparticles in cellular systems The detection of specific target DNA sequences associated with breast cancer using "molecular sentinel" nanoprobes and the use of a plasmonic nanosensor to monitor pH in single cells are presented and discussed.
authors.affiliation: Fitzpatrick Institute for Photonics, Departments of Biomedical Engineering and Chemistry, Duke University, Durham, NC 27708, USA. tuan.vodinh@duke.edu
authors.forename: Tuan
authors.initials: T
authors.lastname: Vo-Dinh
authors.forename: Hsin-Neng
authors.initials: HN
authors.lastname: Wang
authors.forename: Jonathan
authors.initials: J
authors.lastname: Scaffidi
mesh_terms: D004247:DNA / Q000737:chemistry; D006801:Humans; D053768:Metal Nanoparticles / Q000737:chemistry; D055095:Optics and Photonics / Q000295:instrumentation* / Q000379:methods*; D012834:Silver / Q000737:chemistry
chemical_list: D012834:Silver; D004247:DNA