Genetic copy number alterations and IL-13 expression differences in papillary thyroid cancers and benign nodules.
Thyroid nodules were the extremely common endocrine tumors, in which papillary thyroid carcinomas (PTCs) were the most prevalent endocrine malignancy, representing 80-90% of all thyroid malignancies. It was still a dilemma to discriminate PTCs and benign thyroid nodules. With a new molecular genetics technology of Multiplex ligation-dependent probe amplification (MLPA), we investigated 13 PTC and 14 benign nodule tissue samples. The results showed that PTCs had more genetic copy number alteration than benign nodules (P < 0.001). Receiver operating characteristic (ROC) curve analysis suggested that genomic aberrations would provide a moderate accuracy method to discriminate PTCs and benign nodules. The gain of interleukin 13 (IL-13) gene obviously identified the great difference between PTCs and benign nodules. Immunohistochemistry also confirmed significantly higher IL-13 expression in the PTCs (P < 0.001). The current study showed that MLPA should be an effective method to diagnose PTCs and benign thyroid nodules, and also provided a clue to another relationship between IL-13 and PTCs.
Zhao
ZeFei
Z
Shanghai Institute of Endocrinology and Metabolism, Shanghai JiaoTong University School of Medicine, Shanghai, 200025, People's Republic of China.
Wei
Qing
Q
Zhao
Yongju
Y
Sun
Fukang
F
Jin
Xiaolong
X
Cui
Bin
B
Ning
Guang
G
D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000235:genetics*; D002291:Carcinoma, Papillary / Q000235:genetics* / Q000473:pathology; D003937:Diagnosis, Differential; D005260:Female; D018628:Gene Dosage; D005820:Genetic Testing / Q000379:methods; D006801:Humans; D007150:Immunohistochemistry; D018793:Interleukin-13 / Q000235:genetics* / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D009369:Neoplasms / Q000235:genetics* / Q000473:pathology; D013961:Thyroid Gland / Q000378:metabolism / Q000473:pathology; D013964:Thyroid Neoplasms / Q000235:genetics* / Q000473:pathology; D016606:Thyroid Nodule / Q000235:genetics* / Q000473:pathology
D014408:Biomarkers, Tumor; D018793:Interleukin-13