Intracellular Ca2+ signaling and store-operated Ca2+ entry are required in Drosophila neurons for flight.
Neuronal Ca(2+) signals can affect excitability and neural circuit formation. Ca(2+) signals are modified by Ca(2+) flux from intracellular stores as well as the extracellular milieu. However, the contribution of intracellular Ca(2+) stores and their release to neuronal processes is poorly understood. Here, we show by neuron-specific siRNA depletion that activity of the recently identified store-operated channel encoded by dOrai and the endoplasmic reticulum Ca(2+) store sensor encoded by dSTIM are necessary for normal flight and associated patterns of rhythmic firing of the flight motoneurons of Drosophila melanogaster. Also, dOrai overexpression in flightless mutants for the Drosophila inositol 1,4,5-trisphosphate receptor (InsP(3)R) can partially compensate for their loss of flight. Ca(2+) measurements show that Orai gain-of-function contributes to the quanta of Ca(2+)-release through mutant InsP(3)Rs and elevates store-operated Ca(2+) entry in Drosophila neurons. Our data show that replenishment of intracellular store Ca(2+) in neurons is required for Drosophila flight.
Venkiteswaran
Gayatri
G
National Centre for Biological Sciences, Tata Institute of Fundamental Research, Bangalore, India.
Hasan
Gaiti
G
D000483:Alleles; D000818:Animals; D002118:Calcium / Q000378:metabolism*; D020013:Calcium Signaling; D002148:Calmodulin-Binding Proteins / Q000235:genetics / Q000378:metabolism; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism; D004331:Drosophila melanogaster / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D005426:Flight, Animal; D053496:Inositol 1,4,5-Trisphosphate Receptors / Q000378:metabolism; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism; D009474:Neurons / Q000378:metabolism*; D000071740:ORAI1 Protein; D034741:RNA, Small Interfering / Q000235:genetics; D000071737:Stromal Interaction Molecule 1
D002148:Calmodulin-Binding Proteins; D029721:Drosophila Proteins; D053496:Inositol 1,4,5-Trisphosphate Receptors; D008565:Membrane Proteins; D000071740:ORAI1 Protein; D034741:RNA, Small Interfering; C502662:Stim protein, Drosophila; D000071737:Stromal Interaction Molecule 1; C052944:calmodulin acceptor protein 60; C510747:olf186-F protein, Drosophila; D002118:Calcium