title: Global gene analysis of Erigeron canadensis-treated TNF-alpha-, IL-4- and IL-1beta-stimulated A549 human epithelial cells.
abstract: OBJECTIVE
  This study was conducted to evaluate the anti-inflammatory mechanisms of Erigeron canadensis (EC) on the tumor necrosis factor-alpha (TNF-alpha)-, interleukin (IL)-4- and IL-1beta-induced stimulation of A549 cells.
  
  
  METHODS
  In the present study, the anti-inflammatory effects of EC on TNF-alpha-, IL-4- and IL-1beta-induced A549 cells were determined by analyzing eotaxin secretion using ELISA. In addition, the effects of ECon gene expression profiles in stimulated A549 cells were evaluated by microarray analysis.
  
  
  RESULTS
  Oligonucleotide microarray analysis revealed that inflammatory-related genes such as NOS1, NOS2A, IL-1beta, IL-8 and CSF2 and cell adhesion-related genes such as SELE, MMP3, VCAM1, ICAM1, ITGA7 and ITGB2 were downregulated in EC-treated A549 cells that had been pretreated with TNF-alpha, IL-4 and IL-1beta. In addition, significant decreases in Eotaxin, ICAM, VCAM and IL-8 gene expression were observed in EC-treated A549 cells.
  
  
  CONCLUSIONS
  EC has an anti-inflammatory effect in stimulated A549 cells. Microarray-based genomic survey is a high-throughput approach that is suitable for the evaluation of gene expression in cell lines that have been treated with EC.
authors.affiliation: Department of Physiology, BK21 Oriental Medical Science Center, Kyung Hee University, Seoul, Republic of Korea.
authors.forename: Sung-Hwa
authors.initials: SH
authors.lastname: Sohn
authors.forename: Eunjung
authors.initials: E
authors.lastname: Ko
authors.forename: Bang-Gul
authors.initials: BG
authors.lastname: Oh
authors.forename: Jinju
authors.initials: J
authors.lastname: Kim
authors.forename: Eunsung
authors.initials: E
authors.lastname: Choi
authors.forename: Sung-Hoon
authors.initials: SH
authors.lastname: Kim
authors.forename: Yangseok
authors.initials: Y
authors.lastname: Kim
authors.forename: Minkyu
authors.initials: M
authors.lastname: Shin
authors.forename: Moochang
authors.initials: M
authors.lastname: Hong
authors.forename: Hyunsu
authors.initials: H
authors.lastname: Bae
mesh_terms: D000465:Algorithms; D000704:Analysis of Variance; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology*; D045744:Cell Line, Tumor; D054413:Chemokine CCL11 / Q000378:metabolism; D016207:Cytokines / Q000276:immunology*; D019587:Dietary Supplements; D004847:Epithelial Cells / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism; D049919:Erigeron; D005786:Gene Expression Regulation / Q000187:drug effects*; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000235:genetics / Q000378:metabolism; D053583:Interleukin-1beta / Q000276:immunology; D015847:Interleukin-4 / Q000276:immunology; D016209:Interleukin-8 / Q000235:genetics / Q000378:metabolism; D020935:MAP Kinase Signaling System / Q000187:drug effects; D020411:Oligonucleotide Array Sequence Analysis; D010936:Plant Extracts / Q000494:pharmacology*; D011650:Pulmonary Alveoli / Q000166:cytology*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014409:Tumor Necrosis Factor-alpha / Q000276:immunology; D019010:Vascular Cell Adhesion Molecule-1 / Q000235:genetics / Q000378:metabolism
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D054413:Chemokine CCL11; D016207:Cytokines; D053583:Interleukin-1beta; D016209:Interleukin-8; D010936:Plant Extracts; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha; D019010:Vascular Cell Adhesion Molecule-1; D018799:Intercellular Adhesion Molecule-1; D015847:Interleukin-4