Conjugated linoleic acid prevents cell growth and cytokine production induced by TPA in human keratinocytes NCTC 2544.
Conjugated linoleic acid (CLA) is reported to have anti-cancer activity, based on animal and in vitro studies. Since it has been suggested that CLA anti-carcinogenic effect stems from its anti-inflammatory properties, this study investigated whether CLA can prevent cell proliferation induced by TPA in human keratinocytes NCTC 2544 contemporary to inhibition of inflammation. Results obtained showed that CLA prevents increased cell proliferation and production of pro-inflammatory molecules determined by TPA, being this effect due to modulation of PPARs and NFkB activity. The involvement of PPARalpha in CLA effect was demonstrated by adding to the cells an antagonist of PPARalpha.
Martinasso
Germana
G
Dipartimento di Medicina ed Oncologia Sperimentale, Università di Torino, Corso Raffaello 30, 10125 Torino, Italy.
Saracino
Silvia
S
Maggiora
Marina
M
Oraldi
Manuela
M
Canuto
Rosa A
RA
Muzio
Giuliana
G
D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D016207:Cytokines / Q000096:biosynthesis*; D004247:DNA / Q000378:metabolism; D006801:Humans; D015603:Keratinocytes / Q000187:drug effects*; D044243:Linoleic Acids, Conjugated / Q000494:pharmacology*; D016328:NF-kappa B / Q000378:metabolism; D047493:PPAR alpha / Q000037:antagonists & inhibitors / Q000502:physiology; D048068:PPAR-beta / Q000502:physiology; D013755:Tetradecanoylphorbol Acetate / Q000633:toxicity*
D016207:Cytokines; D044243:Linoleic Acids, Conjugated; D016328:NF-kappa B; D047493:PPAR alpha; D048068:PPAR-beta; D004247:DNA; D013755:Tetradecanoylphorbol Acetate