Production of isoflavonoids in callus cultures of Pueraria candollei var. mirifica.
Pueraria candollei Wall. ex Benth. var. mirifica (Airy Shaw & Suvat.) Niyomdham was investigated for callus induction using Murashige and Skoog (MS) medium containing different plant growth regulators. After 8 weeks of culture, 66-100% of leaf or stem explants formed calli. Calli from stem explants cultured on MS medium supplemented with 0.5 mg/l thidiazuron (TDZ) gave the maximum of shoot induction (16%) and the highest level of total isoflavonoids [(50.39 +/- 7.06) mg/g dry wt], which was 7-fold higher than that of the native tuber [(7.04 +/- 0.29) mg/g dry wt]. These results suggest that addition of TDZ to the culture medium markedly enhances the production of isoflavonoids in calli induced from stem explants of P. candollei var. mirifica.
Udomsuk
Latiporn
L
Faculty of Pharmaceutical Sciences, Khon Kaen University, Khon Kaen, 40002, Thailand.
Jarukamjorn
Kanokwan
K
Tanaka
Hiroyuki
H
Putalun
Waraporn
W
D002478:Cells, Cultured; D005419:Flavonoids / Q000096:biosynthesis*; D010937:Plant Growth Regulators / Q000378:metabolism; D018515:Plant Leaves / Q000378:metabolism; D018520:Plant Shoots / Q000254:growth & development / Q000378:metabolism; D018547:Plant Stems / Q000378:metabolism; D010946:Plants, Medicinal / Q000166:cytology / Q000378:metabolism; D029908:Pueraria / Q000166:cytology / Q000378:metabolism*
D005419:Flavonoids; D010937:Plant Growth Regulators