title: [Polymorphism of D10S1248, D2S441, D1S1677 in Hunan Han population].
abstract: OBJECTIVE
  To establish a multiplex set including D10S1248, D2S441, D1S1677 and to investigate the genetic polymorphism of the three miniSTR in Hunan Han population.
  
  
  METHODS
  Three miniSTR loci of 186 unrelated individuals were amplified with different multiplex fluorescence-labeled primers. The amplified products were analyzed by ABI 310 Genetic Analyzer to identify genotype.
  
  
  RESULTS
  Each locus was successfully genotyped. Among the 186 individuals investigated, 9, 7 and 7 alleles, as well as 21, 19 and 15 genotypes were detected at D10S1248, D2S441 and D1S1677, respectively. No significant deviation from Hardy-Weinberg equilibrium was observed. The excluding probability of paternity and the discrimination power were 0,465, 0.491 and 0.361, as well as 0.886, 0.899 and 0.818 for D10S1248, D2S441 and D1S1677, respectively.
  
  
  CONCLUSIONS
  The miniSTR multiplex set can benefit forensic analysis of degraded samples. It has shown good polymorphism in Hunan Han population and can be used in personal identification and paternity test.
authors.affiliation: Hebei Key Laboratory of Forensic Medicine, Department of Forensic Medicine, Hebei Medical University, Shijiazhuang 050017, China. yutongli116@163.com
authors.forename: Xia
authors.initials: X
authors.lastname: Li
authors.forename: Bin
authors.initials: B
authors.lastname: Cong
authors.forename: Shu-Jin
authors.initials: SJ
authors.lastname: Li
authors.forename: Xue
authors.initials: X
authors.lastname: Bai
authors.forename: Li
authors.initials: L
authors.lastname: Xu
authors.forename: Zhi-Ping
authors.initials: ZP
authors.lastname: Hou
authors.forename: Xia
authors.initials: X
authors.lastname: Guo
mesh_terms: D000483:Alleles; D044466:Asian People / Q000235:genetics; D001483:Base Sequence; D002681:China / Q000208:ethnology; D053803:Forensic Genetics; D005828:Genetics, Population; D005838:Genotype; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic / Q000235:genetics*; D017422:Sequence Analysis, DNA; D020080:Tandem Repeat Sequences / Q000235:genetics*