Purification strategy for recombinant forms of the human mitochondrial DNA helicase.
In this chapter, we present a streamlined purification for the production of near-homogeneous and high yield recombinant forms of the human mitochondrial DNA helicase. Minimizing the number of steps and the time elapsed for purification of this enzyme facilitates studies of its structure and mechanism and allows elucidation of native features of both wild-type- and human disease-related forms.
Ziebarth
Tawn D
TD
Department of Biochemistry and Molecular Biology, Michigan State University, East Lansing, MI, USA.
Kaguni
Laurie S
LS
D000251:Adenosine Triphosphatases / Q000378:metabolism; D002846:Chromatography, Affinity; D004265:DNA Helicases / Q000302:isolation & purification* / Q000378:metabolism; D006801:Humans; D008928:Mitochondria / Q000201:enzymology / Q000378:metabolism; D009532:Nickel / Q000737:chemistry; D011994:Recombinant Proteins / Q000302:isolation & purification* / Q000378:metabolism; D012685:Sepharose / Q000031:analogs & derivatives / Q000737:chemistry
D011994:Recombinant Proteins; C037405:heparin-sepharose; D009532:Nickel; D012685:Sepharose; D000251:Adenosine Triphosphatases; D004265:DNA Helicases