Rapid method for measurement of acetylcholinesterase activity.
Fluorescence intensity of thioflavin T fluorogenic label increased significantly as a result of formation of enzyme-inhibitory complex with acetylcholinesterase of human blood erythrocytes. Thioflavin T is a reversible inhibitor, selectively reacting with acetylcholinesterase. Thioflavin T fluorescence intensity is proportional to acetylcholinesterase activity for the studied interval of enzyme activities. A rapid fluorescent method for measuring acetylcholinesterase activity is proposed.
Antokhin
A M
AM
Research Center, Ministry of Defense of the Russian Federation, Moscow, Russia.
Gainullina
E T
ET
Ryzhikov
S B
SB
Taranchenko
V F
VF
Yavaeva
D K
DK
D000110:Acetylcholinesterase / Q000097:blood* / Q000187:drug effects; D052160:Benzothiazoles; D002800:Cholinesterase Inhibitors / Q000494:pharmacology; D004912:Erythrocytes / Q000201:enzymology*; D005456:Fluorescent Dyes / Q000737:chemistry*; D006801:Humans; D013050:Spectrometry, Fluorescence / Q000379:methods*; D013844:Thiazoles / Q000737:chemistry*
D052160:Benzothiazoles; D002800:Cholinesterase Inhibitors; D005456:Fluorescent Dyes; D013844:Thiazoles; C009462:thioflavin T; D000110:Acetylcholinesterase