Proteomic analysis of indium embryotoxicity in cultured postimplantation rat embryos.
Indium embryotoxicity was investigated by proteomic analysis with two-dimensional electrophoresis of rat embryos cultured from day 10.5 of gestation for 24h in the presence of 50 microM indium trichloride. In the embryo proper, indium increased quantity of several protein spots including those identified as serum albumin, phosphorylated cofilin 1, phosphorylated destrin and tyrosyl-tRNA synthetase. The increased serum albumin, derived from the culture medium composed of rat serum, may decrease the toxicity of indium. The increase of phosphorylated cofilin 1 might be involved in dysmorphogenicity of indium through perturbation of actin functions. In the yolk sac membrane, indium induced quantitative and qualitative changes in the protein spots. Proteins from appeared spots included stress proteins, and those from decreased or disappeared spots included serum proteins, glycolytic pathway enzymes and cytoskeletal proteins, indicating yolk sac dysfunction. Thus, several candidate proteins that might be involved in indium embryotoxicity were identified.
Usami
Makoto
M
Division of Pharmacology, National Institute of Health Sciences, 1-18-1, Kamiyoga, Setagaya, Tokyo 158-8501, Japan. usami@nish.go.jp
Nakajima
Mikio
M
Mitsunaga
Katsuyoshi
K
Miyajima
Atsuko
A
Sunouchi
Momoko
M
Doi
Osamu
O
D000818:Animals; D051338:Cofilin 1 / Q000378:metabolism; D051345:Destrin / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D004527:Egg Proteins / Q000187:drug effects / Q000378:metabolism; D046149:Embryo Culture Techniques; D004622:Embryo, Mammalian / Q000187:drug effects* / Q000378:metabolism; D047108:Embryonic Development / Q000187:drug effects*; D005260:Female; D007204:Indium / Q000494:pharmacology / Q000633:toxicity*; D008565:Membrane Proteins / Q000187:drug effects / Q000378:metabolism; D009924:Organ Culture Techniques; D011247:Pregnancy; D020543:Proteome / Q000032:analysis* / Q000187:drug effects; D040901:Proteomics; D051381:Rats; D017208:Rats, Wistar; D015017:Yolk Sac / Q000737:chemistry / Q000187:drug effects
D051338:Cofilin 1; D051345:Destrin; D004527:Egg Proteins; D008565:Membrane Proteins; D020543:Proteome; D007204:Indium