[Multilocus sequence typing of methicillin-resistant Staphylococcus aureus].
OBJECTIVE
To analyze multilocus sequence typing (MLST) of methicillin-resistant Staphylococcus aureus (MRSA) strains in 2000 and 2005, and get a primary knowledge of MLST Characterization of MRSA.


METHODS
Sequence analysis was conducted on seven allelic genes of 29 methicillin-resistant Staphylococcus aureus strains and 2 methicillin-sensitive Staphylococcus aureus (MSSA) strains and the allelic profiles were gained from internet database.


RESULTS
All 12 MRSA strains in 2000 were sequence type (ST) 239 and 10 MRSA strains in 2005 were ST239, while 7 MRSA strains in 2005 were new types, ST5 (41.18%, 7/17). ST6 and ST630 were allelic profiles of 2 MSSA strains. ST239 was the most prevalent allelic profile (75.86%, 22/29), while ST5 was the second prevalent allelic profile (24.14%, 7/29) among all isolates.


CONCLUSIONS
ST239 and ST5 are the most prevalent MRSA clones in this research. MRSA strains have different allelic profile from MSSA strains. MLST might provide an unambiguous method for assigning MRSA and MSSA isolates to known clones or assigning them as novel clones via the internet. Further studies need to be taken by increasing strains.
Yan
Xiao-mei
XM
National Institute of Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China.
Gu
Yi-xin
YX
He
Li-hua
LH
Zhang
Hui-fang
HF
Zhang
Jian-zhong
JZ
D015373:Bacterial Typing Techniques; D001483:Base Sequence; D004269:DNA, Bacterial / Q000235:genetics; D005798:Genes, Bacterial; D055624:Methicillin-Resistant Staphylococcus aureus / Q000145:classification* / Q000187:drug effects / Q000235:genetics*
D004269:DNA, Bacterial