title: Influence of surface wettability on competitive protein adsorption and initial attachment of osteoblasts.
abstract: This study investigated the influence of surface wettability on competitive protein adsorption and the initial attachment of osteoblasts. A thin-film coating of hexamethyldisiloxane (HMDSO) and subsequent O(2)-plasma treatment was carried out on substrates with a mirror surface in order to create a wide range of wettabilities. The adsorption behavior of fibronectin (Fn) and albumin (Alb) in both individual and competitive mode, and the initial attachment of mouse osteoblastic cells (MC3T3-E1) over a wide range of wettabilities were investigated. The contact angle of HMDSO coatings without O(2)-plasma treatment against double-distilled water was more than 100 degrees, whereas it dramatically decreased after the O(2)-plasma treatment to almost 0 degrees, resulting in super-hydrophilicity. Individually, Fn adsorption showed a biphasic inclination, whereas Alb showed greater adsorption to hydrophobic surfaces. In the competitive mode, in a solution containing both Fn and Alb, Fn showed greater adsorption on hydrophilic surfaces, whereas Alb predominantly adsorbed on hydrophobic surfaces. The initial attachment of osteoblastic cells increased with an increase in surface wettability, in particular, on a super-hydrophilic surface, which correlated well with Fn adsorption in the competitive mode. These results suggest that Fn adsorption may be responsible for increasing cell adhesion on hydrophilic surfaces in a body fluid or culture media under physiological conditions.
authors.affiliation: Department of Oral and Maxillofacial Surgery, School of Stomatology, Fourth Military Medical University, 145 West Changle Road, Xi'an 710032, People's Republic of China.
authors.forename: Jianhua
authors.initials: J
authors.lastname: Wei
authors.forename: Toshio
authors.initials: T
authors.lastname: Igarashi
authors.forename: Naoto
authors.initials: N
authors.lastname: Okumori
authors.forename: Takayasu
authors.initials: T
authors.lastname: Igarashi
authors.forename: Takashi
authors.initials: T
authors.lastname: Maetani
authors.forename: Baolin
authors.initials: B
authors.lastname: Liu
authors.forename: Masao
authors.initials: M
authors.lastname: Yoshinari
mesh_terms: D000327:Adsorption; D000418:Albumins / Q000737:chemistry*; D000818:Animals; D002448:Cell Adhesion; D002478:Cells, Cultured; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D005353:Fibronectins / Q000737:chemistry*; D016650:Fluorescein-5-isothiocyanate / Q000378:metabolism; D019084:Fluorescent Antibody Technique, Indirect; D005456:Fluorescent Dyes / Q000378:metabolism; D057927:Hydrophobic and Hydrophilic Interactions; D051379:Mice; D010006:Osteoblasts / Q000166:cytology / Q000502:physiology* / Q000648:ultrastructure; D012235:Rhodamines / Q000378:metabolism; D012833:Siloxanes / Q000737:chemistry*; D013499:Surface Properties; D013997:Time Factors; D017155:Wettability
chemical_list: D000418:Albumins; D020099:Coated Materials, Biocompatible; D005353:Fibronectins; D005456:Fluorescent Dyes; D012235:Rhodamines; D012833:Siloxanes; C015917:hexamethyldisiloxane; D016650:Fluorescein-5-isothiocyanate