[Human foreskin acellular matrix graft: a good scaffold for urethral tissue engineering].
OBJECTIVE
To provide evidence of using the human foreskin acellular matrix graft for urethral tissue engineering. METH-ODS: The human foreskin acellular matrix graft was prepared, its safety and biocompatibility as urethral material were determined by histological observation, cytotoxicity test using primary epithelial cells and experiment in vivo.


RESULTS
Intact cells were absent from the foreskin acellular matrix graft. The cytotoxicity test showed that the relative growth rate of the cells was between 75% and 99%, and the cytotoxicity of the foreskin acellular matrix graft was grade 1, consistent with the national standard. With the lengthening of time, the foreskin acellular matrix graft became perfectly compatible with the urothelial cells and the urethral multi-layer structure was restored to normal gradually.


CONCLUSIONS
The human foreskin acellular matrix graft, with its low antigenicity and good biocompatibility, could be a good scaffold for urethral tissue engineering.
Jiang
Hua
H
Department of Urology, The Ninth People's Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200011, China.
Ma
Li-Min
LM
Zhou
Juan
J
Lu
Mu-Jun
MJ
Cai
Zhi-Kang
ZK
Wang
Hao
H
Da
Jun
J
Chen
Qi
Q
Wang
Zhong
Z
D002478:Cells, Cultured; D052816:Foreskin / Q000166:cytology / Q000637:transplantation*; D006801:Humans; D008297:Male; D019651:Plastic Surgery Procedures / Q000379:methods*; D046509:Tissue Culture Techniques; D023822:Tissue Engineering / Q000379:methods*; D014521:Urethra / Q000601:surgery*