title: The apical/basal-polarity determinant Scribble cooperates with the PCP core factor Stbm/Vang and functions as one of its effectors.
abstract: Most tissues display several features of cellular polarization. Besides the ubiquitous epithelial polarization in the Apical-Basal (A/B) axis, many epithelia (and associated organs) display a Planar Cell Polarization (PCP). Recently, a crosstalk between the PCP and A/B polarity determinants has been suggested, i.e. the activity or stability of the PCP factor Frizzled is regulated by the A/B determinants aPKC and Bazooka in the Drosophila eye. We have systematically investigated genetic and physical interactions between the Drosophila A/B factors and the core PCP component Strabismus (Stbm)/Van Gogh (Vang). The A/B determinant Scribble was found to interact both genetically and physically with Stbm/Vang. We demonstrate that Scribble binds Stbm/Vang through its PDZ domain 3 and that it cooperates with Stbm/Vang in PCP establishment. Our data indicate that Scribble, in addition to its role in A/B polarity, has a distinct requirement in PCP establishment in the Drosophila eye and wing. We define a scribble allele that is largely PCP specific. Our data show that Scribble is part of the Stbm/Vang PCP complex and further suggest that it might act as an effector of Stbm/Vang during PCP establishment.
authors: Department of Developmental and Regenerative Biology, Mount Sinai School of Medicine, Annenberg Bldg 18-92, One Gustave L. Levy Place, New York, NY 10029, USA.
authors: Jean-Remy
authors: JR
authors: Courbard
authors: Alexandre
authors: A
authors: Djiane
authors: Jun
authors: J
authors: Wu
authors: Marek
authors: M
authors: Mlodzik
mesh_terms: D000818:Animals; D019521:Body Patterning / Q000502:physiology; D016764:Cell Polarity / Q000502:physiology; D054910:Compound Eye, Arthropod / Q000166:cytology / Q000254:growth & development* / Q000378:metabolism; D004330:Drosophila / Q000166:cytology / Q000254:growth & development* / Q000378:metabolism; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism*; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D009154:Mutation; D011485:Protein Binding; D014921:Wings, Animal / Q000166:cytology / Q000254:growth & development* / Q000378:metabolism
chemical_list: D029721:Drosophila Proteins; D008565:Membrane Proteins; C404683:Scrib protein, Drosophila; C111638:Vang protein, Drosophila