[The tear proteomics analysis of conjunctivochalasis].
OBJECTIVE
To analysis tear proteins by using shotgun strategy, and to study pathogenesis mechanism of conjunctivochalasis by comparing tear proteins between the conjunctivochalasis and normal body.


METHODS
Tears were obtained from 8 conjunctivochalasis cases and 8 normal controls. Fifteen microliters of tears was collected by microcapillary tubes from each eye. Shotgun strategy was used for tear protein analysis. Trypsin digestion in-solution, separation of peptide mixture by reverse-phase high-pressure liquid chromatograph (RP-HPLC) followed by electrospray ionization mass spectrometry (ESI-MS/MS) identification and bioinformation analysis were used in this study.


RESULTS
Three hundred and fifty-six proteins were excised in conjunctivochalasis patients and 352 proteins in the normal controls, and among them 119 proteins were the same. The functions of tear proteins were classified with GOA, which found some apoptosis regulation proteins and apoptosis related proteins and inflammatory response related proteins in conjuntivochalasis but not in normal controls group. Defensin was also found in conjuntivochalasis.


CONCLUSIONS
Shotgun strategy can separate and analysis tear proteins effectively, which provide a new method for tear protein component of conjunctivochalasis. The special component in conjunctivochalasis tear show conjunctivochalasis maybe related to cell apoptosis and inflammatory.
Zhang
Xing-ru
XR
Department of Ophthalmology, the Affiliated Putuo Hospita of Shanghai Chinese Traditional Medicine University, Shanghai 200062, China. zhangxingru928@hotmail.com
Xiang
Min-hong
MH
Wu
Qing-qing
QQ
Li
Qing-song
QS
Xu
Yan
Y
Sun
An-guo
AG
D000368:Aged; D000369:Aged, 80 and over; D016022:Case-Control Studies; D003229:Conjunctival Diseases / Q000378:metabolism*; D005136:Eye Proteins / Q000378:metabolism*; D005260:Female; D006801:Humans; D008297:Male; D040901:Proteomics; D013666:Tears / Q000378:metabolism*
D005136:Eye Proteins; C042434:tear proteins