Production of angiotensin I-converting enzyme inhibitory peptides from defatted canola meal.
To simplify the method of ACE-inhibitory peptide production, defatted canola meal was subjected to enzymatic proteolysis. Alcalase 2.4 L and protease M "Amano" were found to be the most efficient enzymes in releasing ACE-inhibitory peptides from canola proteins among 13 tested enzymes. The IC(50) values of canola protein hydrolysates ranged from 18.1 to 82.5 microg protein/mL. Differences in ACE-inhibitory activities of various protein hydrolysates reflected varied enzyme specificities. A positive correlation was determined between ACE-inhibitory activity and the degree of hydrolysis (r=0.5916, p<0.001). Ion-exchange chromatography of canola protein hydrolysate increased the protein content greater than 95% without loss of ACE-inhibitory activity. This fraction was resistant to the degradation of gastrointestinal enzyme and ACE during in vitro incubation and may be a useful ingredient in the formulation of hypotensive functional food products.
Wu
Jianping
J
Agriculture and Agri-Food Canada, Saskatoon Research Station, 107 Science Place, Saskatoon, SK, Canada S7N OX2. jwu3@ualberta.ca
Aluko
Rotimi E
RE
Muir
Alister D
AD
D000806:Angiotensin-Converting Enzyme Inhibitors / Q000138:chemical synthesis*; D005591:Chemical Fractionation; D000431:Ethanol; D005229:Fatty Acids, Monounsaturated / Q000737:chemistry*; D006868:Hydrolysis / Q000187:drug effects; D020128:Inhibitory Concentration 50; D007474:Ion Exchange; D008567:Membranes, Artificial; D008970:Molecular Weight; D010455:Peptides / Q000138:chemical synthesis*; D011492:Protein Hydrolysates / Q000494:pharmacology; D000074262:Rapeseed Oil; D014462:Ultrafiltration
D000806:Angiotensin-Converting Enzyme Inhibitors; D005229:Fatty Acids, Monounsaturated; D008567:Membranes, Artificial; D010455:Peptides; D011492:Protein Hydrolysates; D000074262:Rapeseed Oil; D000431:Ethanol