Electrospray mass spectrometry for quantitative plasma proteome analysis.
Electrospray ionization mass spectrometry (ESI-MS) is an efficient soft ionization procedure for macro biomolecules. However, it is a rather delicate process to produce charged molecules for mass-to-charge ratio (m/z) based measurement. In this chapter, the mechanism of ESI is briefly presented, and the experimental pipeline for quantitative profiling of plasma proteins (prefractionation immunodepletion, protein isotope tagging, 2D-HPLC separation of intact proteins, and LC-MS) is presented as applied by our group in studies of cancer biomarker discovery.
Wang
Hong
H
Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA.
Hanash
Sam
S
D001798:Blood Proteins / Q000032:analysis*; D002846:Chromatography, Affinity; D002853:Chromatography, Liquid / Q000379:methods; D015180:Electrophoresis, Gel, Two-Dimensional; D006801:Humans; D010446:Peptide Fragments / Q000032:analysis; D010449:Peptide Mapping; D020543:Proteome / Q000032:analysis*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
D001798:Blood Proteins; D010446:Peptide Fragments; D020543:Proteome