title: Combining electron crystallography and X-ray crystallography to study the MlotiK1 cyclic nucleotide-regulated potassium channel.
abstract: We have recently reported the X-ray structure of the cyclic nucleotide-regulated potassium channel, MlotiK1. Here we describe the application of both electron and X-ray crystallography to obtain high quality crystals. We suggest that the combined application of these techniques provides a useful strategy for membrane protein structure determination. We also present negative stain projection and cryo-data projection maps. These maps provide new insights about the properties of the MlotiK1 channel. In particular, a comparison of a 9A cryo-data projection with calculated model maps strongly suggests that there is a very weak interaction between the pore and the S1-S4 domains of this 6 TM tetrameric cation channel and that the S1-S4 domains can adopt multiple orientations relative to the pore.
authors: Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT 06520, USA. gina_clayton@merck.com
authors: Gina M
authors: GM
authors: Clayton
authors: Steve G
authors: SG
authors: Aller
authors: Jimin
authors: J
authors: Wang
authors: Vinzenz
authors: V
authors: Unger
authors: João H
authors: JH
authors: Morais-Cabral
mesh_terms: D003461:Crystallography; D018360:Crystallography, X-Ray; D004583:Electrons; D008958:Models, Molecular; D015221:Potassium Channels / Q000737:chemistry*; D011487:Protein Conformation
chemical_list: D015221:Potassium Channels