The bactericidal effect of a complement-independent antibody is osmolytic and specific to Borrelia.
A complement-independent bactericidal IgG1 against the OspB of Borrelia burgdorferi increased the permeability of the outer membrane through the creation of openings of 2.8 - 4.4 nm, resulting in its osmotic lysis. Cryo-electron microscopy and tomography demonstrated that exposure to the antibody causes the formation of outer membrane projections and large breaks which may precede the increase in permeability of the outer membrane. The bactericidal effect of this antibody is not transferable to Escherichia coli expressing rOspB on its outer membrane. Additionally, the porin P66, the only protein that coprecipitated with OspB, is dispensable for the bactericidal mechanism.
LaRocca
Timothy J
TJ
Department of Molecular Genetics and Microbiology, Center for Infectious Diseases, Stony Brook University, Stony Brook, NY 11794-5120, USA.
Holthausen
David J
DJ
Hsieh
Chyongere
C
Renken
Christian
C
Mannella
Carmen A
CA
Benach
Jorge L
JL
D000900:Anti-Bacterial Agents / Q000276:immunology / Q000494:pharmacology*; D000911:Antibodies, Monoclonal / Q000276:immunology / Q000494:pharmacology*; D000942:Antigens, Bacterial / Q000276:immunology; D001425:Bacterial Outer Membrane Proteins / Q000276:immunology; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D001433:Bacteriolysis / Q000187:drug effects*; D025065:Borrelia burgdorferi / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure; D002463:Cell Membrane Permeability / Q000187:drug effects; D020285:Cryoelectron Microscopy; D004305:Dose-Response Relationship, Drug; D004926:Escherichia coli / Q000187:drug effects; D015151:Immunoblotting; D007074:Immunoglobulin G / Q000276:immunology / Q000494:pharmacology; D047468:Immunoprecipitation; D009154:Mutation; D009997:Osmotic Pressure; D018272:Porins / Q000235:genetics / Q000378:metabolism
D000900:Anti-Bacterial Agents; D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D007074:Immunoglobulin G; C107893:Oms66 protein, Borrelia; D018272:Porins; C077030:OspB protein, Borrelia burgdorferi