title: Metformin improves postprandial glucose homeostasis in rainbow trout fed dietary carbohydrates: a link with the induction of hepatic lipogenic capacities?
abstract: Carnivorous fish are poor users of dietary carbohydrates and are considered to be glucose intolerant. In this context, we have tested, for the first time in rainbow trout, metformin, a common anti-diabetic drug, known to modify muscle and liver metabolism and to control hyperglycemia in mammals. In the present study, juvenile trout were fed with very high levels of carbohydrates (30% of the diet) for this species during 10 days followed by feeding with pellets supplemented with metformin (0.25% of the diet) for three additional days. Dietary metformin led to a significant reduction in postprandial glycemia in trout, demonstrating unambiguously the hypoglycemic effect of this drug. No effect of metformin was detected on mRNA levels for glucose transporter type 4 (GLUT4), or enzymes involved in glycolysis, mitochondrial energy metabolism, or on glycogen level in the white muscle. Expected inhibition of hepatic gluconeogenic (glucose-6-phosphatase, fructose-1,6-bisphosphatase, and phosphoenolpyruvate carboxykinase) mRNA levels was not found, showing instead paradoxically higher mRNA levels for these genes after drug treatment. Finally, metformin treatment was associated with higher mRNA levels and activities for lipogenic enzymes (fatty acid synthase and glucose-6-phosphate dehydrogenase). Overall, this study strongly supports that the induction of hepatic lipogenesis by dietary glucose may permit a more efficient control of postprandial glycemia in carnivorous fish fed with high carbohydrate diets.
authors: Institut National de la Recherche Agronomique, Joint Research Unit 1067 Nutrition Aquaculture et Génomique, Saint-Pée-sur-Nivelle, France. panserat@st-pee.inra.fr
authors: S
authors: S
authors: Panserat
authors: S
authors: S
authors: Skiba-Cassy
authors: I
authors: I
authors: Seiliez
authors: M
authors: M
authors: Lansard
authors: E
authors: E
authors: Plagnes-Juan
authors: C
authors: C
authors: Vachot
authors: P
authors: P
authors: Aguirre
authors: L
authors: L
authors: Larroquet
authors: G
authors: G
authors: Chavernac
authors: F
authors: F
authors: Medale
authors: G
authors: G
authors: Corraze
authors: S
authors: S
authors: Kaushik
authors: T W
authors: TW
authors: Moon
mesh_terms: D000824:Animal Nutritional Physiological Phenomena; D000818:Animals; D001786:Blood Glucose / Q000187:drug effects* / Q000378:metabolism; D004040:Dietary Carbohydrates / Q000008:administration & dosage / Q000378:metabolism*; D004734:Energy Metabolism / Q000187:drug effects* / Q000235:genetics; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D006706:Homeostasis; D007004:Hypoglycemic Agents / Q000494:pharmacology*; D050155:Lipogenesis / Q000187:drug effects* / Q000235:genetics; D008099:Liver / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D008687:Metformin / Q000494:pharmacology*; D017686:Oncorhynchus mykiss / Q000378:metabolism*; D019518:Postprandial Period; D012333:RNA, Messenger / Q000378:metabolism; D013997:Time Factors
chemical_list: D001786:Blood Glucose; D004040:Dietary Carbohydrates; D007004:Hypoglycemic Agents; D012333:RNA, Messenger; D008687:Metformin