Long lasting recruitment of immune cells and altered epi-perineurial thickness in focal nerve inflammation induced by complete Freund's adjuvant.
Immune-mediated nerve inflammation is involved in many painful states in humans, and causes axonal and behavioral changes in rats. While models of nerve inflammation have been characterized using electrophysiological and behavioral methods, the presence of immune cells has not been fully assessed. We inflamed rat sciatic nerves using complete Freund's adjuvant and quantified the presence of ED-1 macrophages and TCR-alphabeta T-cells for up to 12 weeks. We report that these immune cells are prominent extraneurally up to 12 weeks following the induction of inflammation. This observation does not easily correlate with inflammation-induced axonal mechanical sensitivity, which peaks within 1 week and is resolved after 8 weeks.
Bove
Geoffrey M
GM
Dept. of Research Administration, University of Southern Maine, 178 Science Building, P.O. Box 9300, Portland, ME 04104-9300, USA. geoffreybove@gmail.com
Weissner
Wendy
W
Barbe
Mary F
MF
D000818:Animals; D002634:Chemotaxis, Leukocyte / Q000187:drug effects / Q000276:immunology*; D004195:Disease Models, Animal; D005620:Freund's Adjuvant / Q000494:pharmacology; D008213:Lymphocyte Activation / Q000187:drug effects / Q000276:immunology*; D008264:Macrophages / Q000187:drug effects / Q000276:immunology / Q000378:metabolism; D008297:Male; D009443:Neuritis / Q000139:chemically induced / Q000276:immunology / Q000503:physiopathology; D010525:Peripheral Nerves / Q000187:drug effects / Q000276:immunology* / Q000473:pathology; D051381:Rats; D017208:Rats, Wistar; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000378:metabolism; D020426:Sciatic Neuropathy / Q000139:chemically induced / Q000276:immunology / Q000503:physiopathology; D013601:T-Lymphocytes / Q000187:drug effects / Q000276:immunology / Q000378:metabolism; D013995:Time; D013997:Time Factors
D016693:Receptors, Antigen, T-Cell, alpha-beta; D005620:Freund's Adjuvant