Modulation of the substance P release from cultured rat primary afferent neurons by zinc ions.
The present study was conducted to determine whether zinc ions are involved in the modulation of substance P release from cultured rat dorsal root ganglion (DRG) neurons. We show here that that lower concentrations (10(-8) - 10(-7) M) of zinc ions may evoke an extracellular Ca(2+) influx through L-, N-, and T-type voltage-dependent Ca(2+) channels, thereby increasing the substance P release from cultured DRG neurons, but higher concentrations (10(-6) - 10(-4) M) of zinc ions attenuate or even completely mask these responses. Zinc ions therefore seem to be an important modulator of substance P release from primary afferent neurons.
Tang
He-Bin
HB
College of Pharmacy, South-Central University for Nationalities, Wuhan, China.
Miyano
Kanako
K
Nakata
Yoshihiro
Y
D000818:Animals; D015220:Calcium Channels / Q000378:metabolism; D002412:Cations / Q000494:pharmacology; D002478:Cells, Cultured; D002712:Chlorides / Q000494:pharmacology*; D005727:Ganglia, Spinal / Q000166:cytology / Q000187:drug effects; D009475:Neurons, Afferent / Q000378:metabolism*; D011863:Radioimmunoassay; D051381:Rats; D017208:Rats, Wistar; D013373:Substance P / Q000378:metabolism*; D017967:Zinc Compounds / Q000494:pharmacology*
D015220:Calcium Channels; D002412:Cations; D002712:Chlorides; D017967:Zinc Compounds; D013373:Substance P; C016837:zinc chloride