The effect of resveratrol in tracheal tissue of rats exposed to cigarette smoke.
OBJECTIVE
The aim of this study was to evaluate the effect of resveratrol on the tracheal tissue of rats exposed to cigarette smoke.


METHODS
40 adult Wistar albino rats were divided into four groups for an experiment of 6 weeks. Animals in group 1 were controls (n = 10). Rats in group 2 were exposed to cigarette smoke only, and rats in group 3 received daily intraperitoneal injections of resveratrol (10 mg/kg/d). Animals in group 4 were exposed to both cigarette smoke and intraperitoneal injections of resveratrol. Rats of all groups were sacrificed using cervical dislocation. The tracheas were removed and embedded in paraffin blocks. Sections of 4-5 mum thickness were prepared from the blocks. These sections were stained with hematoxylin and eosin, periodic acid-Schiff, and Alcian blue and viewed with a Leica DFC 280 light microscope.


RESULTS
Tracheal sections showed that, in group 2 (cigarette smoke group), there was desquamation of epithelial cells into the tracheal lumen, loss of cilia in the epithelial layer, an increase of goblet cells, activation of serous glands at the submucosa, and cell infiltration. In group 4 (cigarette smoke + resveratrol group), all these findings also existed but only a few sections were affected. It was observed that cigarette smoking caused morphological changes such as epithelial degeneration in the upper airway. These morphological changes were correlated with the amount of toxic substances in the cigarette smoke.


CONCLUSIONS
We found that resveratrol had a preventive role in the histopathological changes caused by cigarette smoking in the rat trachea.
Kurus
Meltem
M
Department of Histology-Embryology, School of Medicine, Inonu University, Malatya, Turkey. histomelita@yahoo.com
Firat
Yezdan
Y
Cetin
Asli
A
Kelles
Mehmet
M
Otlu
Ali
A
D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology*; D000975:Antioxidants / Q000494:pharmacology*; D001273:Atmosphere Exposure Chambers; D004847:Epithelial Cells / Q000473:pathology; D019570:Inhalation Exposure; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D000077185:Resveratrol; D013267:Stilbenes / Q000494:pharmacology*; D014028:Tobacco Smoke Pollution / Q000009:adverse effects*; D014132:Trachea / Q000187:drug effects / Q000473:pathology*
D000894:Anti-Inflammatory Agents, Non-Steroidal; D000975:Antioxidants; D013267:Stilbenes; D014028:Tobacco Smoke Pollution; D000077185:Resveratrol