title: Induced pluripotent stem cells and embryonic stem cells are distinguished by gene expression signatures.
abstract: Induced pluripotent stem cells (iPSCs) outwardly appear to be indistinguishable from embryonic stem cells (ESCs). A study of gene expression profiles of mouse and human ESCs and iPSCs suggests that, while iPSCs are quite similar to their embryonic counterparts, a recurrent gene expression signature appears in iPSCs regardless of their origin or the method by which they were generated. Upon extended culture, hiPSCs adopt a gene expression profile more similar to hESCs; however, they still retain a gene expression signature unique from hESCs that extends to miRNA expression. Genome-wide data suggested that the iPSC signature gene expression differences are due to differential promoter binding by the reprogramming factors. High-resolution array profiling demonstrated that there is no common specific subkaryotypic alteration that is required for reprogramming and that reprogramming does not lead to genomic instability. Together, these data suggest that iPSCs should be considered a unique subtype of pluripotent cell.
authors: Department of Biological Chemistry, David Geffen School of Medicine, University of California, Los Angeles, CA 90095, USA.
authors: Mark H
authors: MH
authors: Chin
authors: Mike J
authors: MJ
authors: Mason
authors: Wei
authors: W
authors: Xie
authors: Stefano
authors: S
authors: Volinia
authors: Mike
authors: M
authors: Singer
authors: Cory
authors: C
authors: Peterson
authors: Gayane
authors: G
authors: Ambartsumyan
authors: Otaren
authors: O
authors: Aimiuwu
authors: Laura
authors: L
authors: Richter
authors: Jin
authors: J
authors: Zhang
authors: Ivan
authors: I
authors: Khvorostov
authors: Vanessa
authors: V
authors: Ott
authors: Michael
authors: M
authors: Grunstein
authors: Neta
authors: N
authors: Lavon
authors: Nissim
authors: N
authors: Benvenisty
authors: Carlo M
authors: CM
authors: Croce
authors: Amander T
authors: AT
authors: Clark
authors: Tim
authors: T
authors: Baxter
authors: April D
authors: AD
authors: Pyle
authors: Mike A
authors: MA
authors: Teitell
authors: Matteo
authors: M
authors: Pelegrini
authors: Kathrin
authors: K
authors: Plath
authors: William E
authors: WE
authors: Lowry
mesh_terms: D000818:Animals; D002460:Cell Line; D019175:DNA Methylation; D053595:Embryonic Stem Cells / Q000166:cytology / Q000378:metabolism*; D015870:Gene Expression; D020869:Gene Expression Profiling; D042822:Genomic Instability; D006657:Histones / Q000235:genetics; D006801:Humans; D051379:Mice; D035683:MicroRNAs / Q000378:metabolism; D039904:Pluripotent Stem Cells / Q000166:cytology / Q000378:metabolism*; D011401:Promoter Regions, Genetic
chemical_list: D006657:Histones; D035683:MicroRNAs