Characterization of a leucine aminopeptidase of Babesia gibsoni.
Peptidases of parasitic protozoa are currently under intense investigation in order to identify novel virulence factors, drug targets, and vaccine candidates, except in Babesia. Leucine aminopeptidases in protozoa, such as Plasmodium and Leishmania, have been identified to be involved in free amino acid regulation. We report here the molecular and enzymatic characterization, as well as the localization of a leucine aminopeptidase, a member of the M17 cytosolic aminopeptidase family, from B. gibsoni (BgLAP). A functional recombinant BgLAP (rBgLAP) expressed in Escherichia coli efficiently hydrolysed synthetic substrates for aminopeptidase, a leucine substrate. Enzyme activity of the rBgLAP was found to be optimum at pH 8.0 and at 37 degrees C. The substrate profile was slightly different from its homologue in P. falciprum. The activity was also strongly dependent on metal divalent cations, and was inhibited by bestatin, which is a specific inhibitor for metalloprotease. These results indicated that BgLAP played an important role in free amino acid regulation.
Jia
H
H
National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, Obihiro, Hokkaido 080-8555, Japan.
Terkawi
M A
MA
Aboge
G O
GO
Goo
Y-K
YK
Luo
Y
Y
Li
Y
Y
Yamagishi
J
J
Nishikawa
Y
Y
Igarashi
I
I
Sugimoto
C
C
Fujisaki
K
K
Xuan
X
X
D000595:Amino Acid Sequence; D000818:Animals; D000913:Antibodies, Protozoan; D001403:Babesia / Q000201:enzymology*; D003001:Cloning, Molecular; D020224:Expressed Sequence Tags; D005786:Gene Expression Regulation / Q000502:physiology; D007931:Leucyl Aminopeptidase / Q000737:chemistry / Q000378:metabolism*; D051379:Mice; D008813:Mice, Inbred ICR; D008969:Molecular Sequence Data; D010802:Phylogeny; D013379:Substrate Specificity
D000913:Antibodies, Protozoan; D007931:Leucyl Aminopeptidase