Genetic transformation of cork oak (Quercus suber L.) for herbicide resistance.
The bar gene was introduced into the cork oak genome. Cork oak embryogenic masses were transformed using the Agrobacterium strain AGL1 which carried the plasmid pBINUbiBar. This vector harbours the genes, nptII and bar, the latter under control of the maize ubiquitin promoter. The transgenic embryogenic lines were cryopreserved. Varying activities of phosphinothricin acetyl transferase were detected among the lines, which carried 1-4 copies of the insert. Molecular and biochemical assays confirmed the stability and expression of the transgenes 3 months after thawing the cultures. These results demonstrate genetic engineering of herbicide tolerance in Quercus spp.
Alvarez
Rubén
R
Department of Plant Sciences, University of Cambridge, Downing Street, Cambridge CB2 3EA, UK. ra352@cam.ac.uk
Alvarez
José M
JM
Humara
Jaime M
JM
Revilla
Angeles
A
Ordás
Ricardo J
RJ
D000123:Acetyltransferases / Q000235:genetics / Q000378:metabolism; D018628:Gene Dosage; D042822:Genomic Instability; D053209:Herbicide Resistance; D006540:Herbicides / Q000633:toxicity*; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D030821:Plants, Genetically Modified / Q000187:drug effects / Q000235:genetics* / Q000502:physiology*; D010957:Plasmids; D011401:Promoter Regions, Genetic; D029963:Quercus / Q000187:drug effects / Q000235:genetics* / Q000502:physiology*; D012231:Rhizobium / Q000235:genetics; D014170:Transformation, Genetic; D003313:Zea mays / Q000235:genetics
D006540:Herbicides; D010940:Plant Proteins; D000123:Acetyltransferases; C056243:phosphinothricin N-acetyltransferase