(-)-Epigallocatechin-3-gallate reduces experimental colon injury in rats by regulating macrophage and mast cell.
The ameliorative effect of (-)-epigallocatechin-3-gallate (EGCG) on inflammatory bowel disease (IBD) induced by ethanol 2,4,6-trinitrobenzene sulfonic acid (TNBS) was studied in 7-week-old male rats. Intestinal lesions were measured as an increase in myeloperoxidase (MPO) activity in mucosa. The supplementation of EGCG significantly inhibited MPO activity and histamine levels in the distal colon mucosa. The EGCG inhibited macrophage chemotaxis toward N-formyl-L-methionyl-L-leucyl-L-phenylalanine in a concentration-dependent manner. These observations confirmed that EGCG can ameliorate acute experimental colitis by the suppression of mast cells and macrophage activities.
Mochizuki
Miyako
M
Department of Human Life Science, Graduate School of Human Life Science, Gifu Women's University, 80 Taromaru, Gifu 501-2592, Japan.
Hasegawa
Noboru
N
D000818:Animals; D002392:Catechin / Q000031:analogs & derivatives* / Q000494:pharmacology; D002633:Chemotaxis / Q000187:drug effects; D003092:Colitis / Q000139:chemically induced / Q000188:drug therapy* / Q000473:pathology; D006632:Histamine / Q000378:metabolism; D008264:Macrophages / Q000187:drug effects*; D008297:Male; D008407:Mast Cells / Q000187:drug effects*; D009240:N-Formylmethionine Leucyl-Phenylalanine; D009195:Peroxidase / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D014302:Trinitrobenzenesulfonic Acid
D009240:N-Formylmethionine Leucyl-Phenylalanine; D006632:Histamine; D002392:Catechin; D014302:Trinitrobenzenesulfonic Acid; C045651:epigallocatechin gallate; D009195:Peroxidase