2D-IR spectroscopy: ultrafast insights into biomolecule structure and function.
Ultrafast 2D-IR spectroscopy is rapidly becoming a valuable tool for examining the relationship between structure and function of biomolecules. The unique combination of molecular-level structural information and ultrafast time resolution gives previously inaccessible insights relating to the rapid structural fluctuations, vibrational dynamics and solvent-solute interactions of proteins, all of which have important implications for the biological function of these species. In this tutorial review, the method and development of ultrafast 2D-IR spectroscopy is discussed, including an introduction to the two main experimental approaches, double resonance and photon echo 2D-IR, and the extension of the technique to non-equilibrium or transient 2D-IR measurements. The scope of the new information available through 2D-IR spectroscopy is then demonstrated by reference to the current state of the art of 2D-IR studies of molecules of biological interest.
Hunt
Neil T
NT
Department of Physics, University of Strathclyde, Glasgow, UKG4 0NG. nhunt@phys.strath.ac.uk
D000818:Animals; D004247:DNA / Q000737:chemistry; D006801:Humans; D008958:Models, Molecular; D011485:Protein Binding; D011487:Protein Conformation; D011506:Proteins / Q000737:chemistry* / Q000378:metabolism*; D013055:Spectrophotometry, Infrared / Q000295:instrumentation / Q000379:methods*
D011506:Proteins; D004247:DNA