A direct micropipette-based calibration method for atomic force microscope cantilevers.
In this report, we describe a direct method for calibrating atomic force microscope (AFM) cantilevers with the micropipette aspiration technique (MAT). A closely fitting polystyrene bead inside a micropipette is driven by precisely controlled hydrostatic pressures to apply known loads on the sharp tip of AFM cantilevers, thus providing a calibration at the most functionally relevant position. The new method is capable of calibrating cantilevers with spring constants ranging from 0.01 to hundreds of newtons per meter. Under appropriate loading conditions, this new method yields measurement accuracy and precision both within 10%, with higher performance for softer cantilevers. Furthermore, this method may greatly enhance the accuracy and precision of calibration for colloidal probes.
Liu
Baoyu
B
Department of Biomedical Engineering, Washington University, Saint Louis, Missouri 63130, USA.
Yu
Yan
Y
Yao
Da-Kang
DK
Shao
Jin-Yu
JY
D000465:Algorithms; D000704:Analysis of Variance; D002138:Calibration; D004548:Elasticity; D016014:Linear Models; D018625:Microscopy, Atomic Force / Q000295:instrumentation* / Q000379:methods; D055616:Microtechnology; D011312:Pressure; D012399:Rotation; D013696:Temperature