Thickness dependence of optical second harmonic generation in collagen fibrils.
Simultaneous backward and forward second harmonic generations from isolated type-I collagen matrix are observed. Optical interference behaviors of these nonlinear optical signals are studied with accurately determined fibril thickness by an atomic force microscope. The nonlinear emission directions are strongly dependent on the coherent interaction within and between collagen fibrils. A linear relationship is obtained to estimate collagen fibril thickness with nanometer precision noninvasively by evaluating the forward/backward second harmonic generation ratio.
Chu
Shi-Wei
SW
Tai
Shih-Peng
SP
Chan
Ming-Che
MC
Sun
Chi-Kuang
CK
Hsiao
I-Ching
IC
Lin
Chi-Hung
CH
Chen
Yung-Chih
YC
Lin
Bai-Ling
BL