Somatostatin can alter fertility genes expression, oocytes maturation, and embryo development in cattle.
This study was designed to investigate the effect of somatostatin on oocytes maturation and subsequent embryo development in cattle. Bovine granulosa cells separated from oocytes, cultured for 24 h and transfected with pEGFP-N1 vector with mouse SST gene (Experimental) and with out plasmid transfection (Control). RT-PCR and Real-Time PCR were used to estimate the expression of bovine receptors of androgen, estrogen beta, growth hormone, and follicular stimulating hormone. Culture media concentrations of hormones were measured by kits using radioimmunoassay. COCs aspirated from ovaries were co-cultured with granulosa cells layers (transfected or control) at 38.5 degrees C in CO(2) incubator for maturation. We found a significant (2.37X) increase in estrogen receptor beta expression in experimental group. There was a decrease in androgen receptor, growth hormone releasing hormone receptor, and follicular stimulating hormone receptor (P < 0.05). But, 96 h of post transfection, culture media concentration of estradiol-17beta was increased significantly (P < 0.05) and testosterone, growth hormone and follicular stimulating hormone showed opposite trend (P < 0.05) in experimental groups. Co-culture of somatostatin transfected granulosa cells with oocytes, reduced the maturation rate from 70% to 66% but had no effect on subsequent fertilization and embryo development.
Moaeen-ud-Din
Muhammad
M
China Education Ministry's Key Laboratory of Animal Breeding, Genetics and Reproduction, College of Animal Science & Technology, Huazhong Agricultural University, Wuhan, Hubei, P.R. China.
Malik
Nosheen
N
Yang
Li Guo
LG
D000818:Animals; D001483:Base Sequence; D002417:Cattle; D018920:Coculture Techniques; D017931:DNA Primers / Q000235:genetics; D047108:Embryonic Development / Q000235:genetics; D047629:Estrogen Receptor beta / Q000235:genetics; D005260:Female; D005298:Fertility / Q000235:genetics*; D015870:Gene Expression; D006107:Granulosa Cells / Q000378:metabolism; D051379:Mice; D009865:Oocytes / Q000254:growth & development / Q000378:metabolism; D011247:Pregnancy; D011944:Receptors, Androgen / Q000235:genetics; D011962:Receptors, FSH / Q000235:genetics; D011986:Receptors, Somatotropin / Q000235:genetics; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D013004:Somatostatin / Q000235:genetics* / Q000378:metabolism*; D014162:Transfection
D017931:DNA Primers; D047629:Estrogen Receptor beta; D011944:Receptors, Androgen; D011962:Receptors, FSH; D011986:Receptors, Somatotropin; D011994:Recombinant Proteins; D013004:Somatostatin