Chickens and cattle as sources of sporadic domestically acquired Campylobacter jejuni infections in Finland.
A substantial sampling among domestic human campylobacter cases, chicken process lots, and cattle at slaughter was performed during the seasonal peak of human infections. Campylobacter jejuni isolates (n = 419) were subtyped using pulsed-field gel electrophoresis with SmaI, and isolates representing overlapping types (n = 212) were further subtyped using KpnI for restriction. The SmaI/KpnI profiles of 55.4% (97/175) of the human isolates were indistinguishable from those of the chicken or cattle isolates. The overlapping SmaI/KpnI subtypes accounted for 69.8% (30/43) and 15.9% (32/201) of the chicken and cattle isolates, respectively. The occurrence of identical SmaI/KpnI subtypes with human C. jejuni isolates was significantly associated with animal host species (P < 0.001). A temporal association of isolates from chickens and patients was possible in 31.4% (55/175) of the human infections. Besides chickens as sources of C. jejuni in the sporadic infections, the role of cattle appears notable. New approaches to restrict the occurrence of campylobacters in other farm animals may be needed in addition to hygienic measures in chicken production. However, only about half of the human infections were attributable to these sources.
Hakkinen
Marjaana
M
Research Department, Finnish Food Safety Authority Evira, Mustialankatu 3, Helsinki FI-00790, Finland. marjaana.hakkinen@evira.fi
Nakari
Ulla-Maija
UM
Siitonen
Anja
A
D000818:Animals; D000829:Animals, Domestic / Q000382:microbiology; D015373:Bacterial Typing Techniques; D002169:Campylobacter Infections / Q000453:epidemiology* / Q000382:microbiology; D016123:Campylobacter jejuni / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D002417:Cattle / Q000382:microbiology*; D002645:Chickens / Q000382:microbiology*; D015252:Deoxyribonucleases, Type II Site-Specific; D016521:Electrophoresis, Gel, Pulsed-Field; D005387:Finland / Q000453:epidemiology; D005516:Food Microbiology; D005838:Genotype; D006801:Humans; D008460:Meat / Q000382:microbiology*; D011201:Poultry Diseases / Q000382:microbiology; D012621:Seasons
C025651:CCCGGG-specific type II deoxyribonucleases; D015252:Deoxyribonucleases, Type II Site-Specific; C029312:GGTACC-specific type II deoxyribonucleases