title: Gene expression analysis of Paracoccidioides brasiliensis transition from conidium to yeast cell.
abstract: Paracoccidioides brasiliensis infectious process relies on the initial expression of virulence factors that are assumed to be controlled by molecular mechanisms through which the conidia and/or mycelial fragments convert to yeast cells. In order to analyze the profile of the thermally-induced dimorphic gene expression, 48 h C-L transition cultures which had been incubated at 36 degrees C were studied. By this time approximately 50% of the conidial population had already reverted to yeast form cells. At this transition time, an EST-Orestes library was constructed and characterized. As a result, 79 sequences were obtained, of which 39 (49.4%) had not been described previously in other libraries of this fungus and which could represent novel exclusive C-Y transition genes. Two of these sequences are, among others, cholestanol delta-isomerase, and electron transfer flavoprotein-ubiquinoneoxidoreductase (ETF-QO). The other 40/79 (50.6%) sequences were shared with Mycelia (M), Yeast (Y) or Mycelia to yest transition (M-Y) libraries. An important component of this group of sequences is a putative response regulator receiver SKN7, a protein of high importance in stress adaptation and a regulator of virulence in some bacteria and fungi. This is the first report identifying genes expressed during the C-Y transition process, the initial step required to understand the natural history of P. brasiliensis conidia induced infection.
authors: Unidad de Biología Celular y Molecular Corporación para Investigaciones Biológicas, Medellín, Colombia. agarcia@cib.org.co
authors: Ana M
authors: AM
authors: García
authors: Orville
authors: O
authors: Hernández
authors: Beatriz H
authors: BH
authors: Aristizabal
authors: Luciano A
authors: LA
authors: De Souza Bernardes
authors: Rosana
authors: R
authors: Puccia
authors: Tony W
authors: TW
authors: Naranjo
authors: Gustavo H
authors: GH
authors: Goldman
authors: Maria H
authors: MH
authors: Goldman
authors: Luz E
authors: LE
authors: Cano
authors: Angela
authors: A
authors: Restrepo
authors: Juan G
authors: JG
authors: McEwen
mesh_terms: D005656:Fungal Proteins / Q000096:biosynthesis / Q000235:genetics; D020869:Gene Expression Profiling; D015966:Gene Expression Regulation, Fungal; D025282:Mycelium / Q000235:genetics / Q000254:growth & development / Q000502:physiology; D010228:Paracoccidioides / Q000235:genetics / Q000254:growth & development / Q000502:physiology*; D013172:Spores, Fungal / Q000235:genetics / Q000254:growth & development / Q000502:physiology; D015003:Yeasts / Q000235:genetics / Q000254:growth & development / Q000502:physiology
chemical_list: D005656:Fungal Proteins