Identification and quantification of sophorolipid analogs using ultra-fast liquid chromatography-mass spectrometry.
An ultra-fast liquid chromatographic method combined with atmospheric pressure chemical ionization mass detection (UHPLC/APCI-MS) has been developed for the separation and quantification of sophorolipid analogs produced by the yeast Candida bombicola. The sophorolipid mixture was produced by growing the yeast in the presence of glucose and oleic acid under higher aeration. It was found that more than 95% of the analogs are lactonic sophorolipids and all the produced sophorolipids produced were either mono- or di-acetylated. Also observed was a sophorolipid analog with a tri-unsaturated fatty acid, which has not been reported previously.
Ratsep
Peter
P
Shimadzu Scientific Instruments Inc., 262 D Old New Brunswick Rd, Piscataway, NJ 08854, United States.
Shah
Vishal
V
D000332:Aerobiosis; D002175:Candida / Q000737:chemistry* / Q000254:growth & development / Q000378:metabolism; D002853:Chromatography, Liquid / Q000379:methods*; D005947:Glucose / Q000378:metabolism; D006017:Glycolipids / Q000032:analysis*; D013058:Mass Spectrometry / Q000379:methods*; D019301:Oleic Acid / Q000378:metabolism
D006017:Glycolipids; C067958:16-(2'-O-glucopyranosylglucopyranosyloxy)hexadecanoic acid 1',4''-lactone 6',6''-diacetate; D019301:Oleic Acid; D005947:Glucose