Enabling IMAC purification of low abundance recombinant proteins from E. coli lysates.
Magnusdottir
Audur
A
Johansson
Ida
I
Dahlgren
Lars-Göran
LG
Nordlund
Pär
P
Berglund
Helena
H
D002614:Chelating Agents; D002846:Chromatography, Affinity / Q000379:methods*; D004926:Escherichia coli / Q000737:chemistry* / Q000235:genetics*; D049452:Green Fluorescent Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D006639:Histidine / Q000235:genetics; D008970:Molecular Weight; D009532:Nickel; D009842:Oligopeptides / Q000235:genetics; D009997:Osmotic Pressure; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification*
D002614:Chelating Agents; C471213:His-His-His-His-His-His; D009842:Oligopeptides; D011994:Recombinant Proteins; D049452:Green Fluorescent Proteins; D006639:Histidine; D009532:Nickel