title: Inhibition of macrophage activation by the myxoma virus M141 protein (vCD200).
abstract: The M141 protein of myxoma virus (MYXV) is a viral CD200 homolog (also called vOX-2) that inhibits macrophage activation in infected rabbits. Here, we show that murine myeloid RAW 264.7 cells became activated when infected with MYXV in which the M141 gene was deleted (vMyx-M141KO) but not with the parental wild-type MYXV. Moreover, transcript and protein levels of tumor necrosis factor and granulocyte colony-stimulating factor were rapidly upregulated in an NF-kappaB-dependent fashion in the RAW 264.7 cells infected with vMyx-M141KO. M141 protein is present in the virion and counteracts this NF-kappaB activation pathway upon infection with the wild-type MYXV. Our data suggest that upregulation of these classic macrophage-related proinflammatory cytokine markers following infection of myeloid cells with the M141-knockout MYXV is mediated via the rapid activation of the cellular NF-kappaB pathway.
authors: Department of Molecular Genetics and Microbiology, University of Florida, Gainesville, 32610, USA.
authors: Leiliang
authors: L
authors: Zhang
authors: Marianne
authors: M
authors: Stanford
authors: Jia
authors: J
authors: Liu
authors: Catherine
authors: C
authors: Barrett
authors: Lei
authors: L
authors: Jiang
authors: A Neil
authors: AN
authors: Barclay
authors: Grant
authors: G
authors: McFadden
mesh_terms: D000818:Animals; D015703:Antigens, CD; D002460:Cell Line; D016179:Granulocyte Colony-Stimulating Factor / Q000235:genetics; D008262:Macrophage Activation / Q000276:immunology*; D008264:Macrophages / Q000821:virology*; D051379:Mice; D022423:Myeloid Cells / Q000821:virology; D009233:Myxoma virus / Q000276:immunology* / Q000472:pathogenicity; D016328:NF-kappa B / Q000037:antagonists & inhibitors / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics; D015854:Up-Regulation; D014764:Viral Proteins / Q000502:physiology*
chemical_list: D015703:Antigens, CD; C501251:M141R protein, myxoma virus; D016328:NF-kappa B; D014409:Tumor Necrosis Factor-alpha; D014764:Viral Proteins; D016179:Granulocyte Colony-Stimulating Factor; C042739:antigens, CD200