title: Domestic use of a disclosing solution for denture hygiene: a randomised trial.
abstract: OBJECTIVE
  The purpose of this study was to investigate the effect of the domestic use of a disclosing agent for denture hygiene.
  
  
  METHODS
  Completely edentulous participants wearing maxillary dentures were randomly assigned to one of the three intervention groups: (1) Follow-up only (control; n = 12); (2) Oral and denture hygiene instructions (n = 10); (3) Instructions associated with the home use of a disclosing agent (1% neutral red; n = 10). Biofilm coverage area (%) over internal and external surfaces of the maxillary denture was assessed at baseline and after 14 and 90 days. Data were evaluated by generalised estimating equations based on score tests (alpha = 0.05).
  
  
  RESULTS
  The participants presented low changes for areas of biofilm coverage (14 days (%): internal: GI = 1.4 +/- 0.9; GII = 1.5 +/- 1.3; GIII = -0.4 +/- 0.9; external: GI = 1.4 +/- 1.5; GII = 1.5 +/- 1.4; GIII = -0.4 +/- 0.9; 90 days (%): internal: GI = 2.0 +/- 0.9; GII = 2.2 +/- 1.4; GIII = 0.3 +/- 1.0; external: GI = 2.1 +/- 1.4; GII = 2.2 +/- 1.5; GIII = 0.3 +/- 0.9). Changes were similar for the three groups (p = 0.293) and were not influenced by the test time (p = 0.218).
  
  
  CONCLUSIONS
  It can be concluded that the home use of a disclosing agent for denture hygiene does not improve the removal of the biofilm, particularly for patients with adequate oral hygiene habits.
authors: Department of Dental Materials and Prosthodontics, Ribeirão Preto School of Dentistry, University of São Paulo, Ribeirão Preto, Brazil. raphael@forp.usp.br
authors: Raphael F
authors: RF
authors: Souza
authors: Rômulo R
authors: RR
authors: Regis
authors: Cássio
authors: C
authors: Nascimento
authors: Helena F O
authors: HF
authors: Paranhos
authors: Claudia H Lovato
authors: CH
authors: Silva
mesh_terms: D000368:Aged; D018441:Biofilms; D004396:Coloring Agents; D003773:Dental Plaque / Q000382:microbiology / Q000628:therapy; D003802:Dentifrices / Q000627:therapeutic use; D003827:Denture, Complete, Upper; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D008297:Male; D008875:Middle Aged; D009499:Neutral Red; D009910:Oral Hygiene; D010353:Patient Education as Topic; D016037:Single-Blind Method; D013499:Surface Properties; D014099:Toothbrushing / Q000295:instrumentation; D016896:Treatment Outcome
chemical_list: D004396:Coloring Agents; D003802:Dentifrices; D009499:Neutral Red