title: Characterization in vivo and in vitro of a strain of Leishmania (Viannia) shawi from the Amazon Region.
abstract: The present study analyses complement resistance, cell surface carbohydrates expression, lipidic composition and morphology in vivo and in vitro, of Leishmania (Viannia) shawi, a parasite identified in the Amazon region, Pará state, in 1989. We demonstrated that promastigotes in the stationary (STAT) growth phase are more resistant to complement lysis than in the logarithmic (LOG) growth phase. Ultrastructural analyses and imidazol technique showed accumulation of lipids in STAT growth phase promastigotes, which was confirmed by biochemical approach. Light and electron microscopy of skin lesion in hamster footpads caused by promastigotes in STAT growth phase, 90 days post inoculation, showed amastigotes inside of macrophage and free in the tissue surrounded by collagen fibers as well as extensive inflammatory reaction with tissue destruction. We also demonstrated, using lectins by agglutination assays and flow cytometry, the presence of fucose, mannose and/or glucose carbohydrate residues on the surface of LOG and STAT promastigotes. The results constitute the first characterization essay combining biochemical and morphological approaches dedicated to LOG and STAT growth phase promastigotes of L. (V) shawi contributing for a better knowledge of this poorly studied species of the New World.
authors: Instituto Evandro Chagas, Unidade de Microscopia Eletrônica, Av. Almirante Barroso 492, Bairro Marco, 66090-000, Belém, Pará, Brazil.
authors: Patrícia K S
authors: PK
authors: Ramos
authors: José A P
authors: JA
authors: Diniz
authors: Edilene O
authors: EO
authors: Silva
authors: Juarez A S
authors: JA
authors: Quaresma
authors: Elvira M
authors: EM
authors: Saraiva
authors: Sérgio H
authors: SH
authors: Seabra
authors: Geórgia C
authors: GC
authors: Atella
authors: Wanderley
authors: W
authors: de Souza
mesh_terms: D000818:Animals; D001938:Brazil; D002241:Carbohydrates / Q000032:analysis / Q000737:chemistry; D003165:Complement System Proteins / Q000276:immunology; D006224:Cricetinae; D006168:Guinea Pigs; D006801:Humans; D037102:Lectins / Q000378:metabolism; D007891:Leishmania / Q000145:classification* / Q000254:growth & development / Q000472:pathogenicity* / Q000648:ultrastructure; D016773:Leishmaniasis, Cutaneous / Q000276:immunology / Q000469:parasitology / Q000473:pathology; D008647:Mesocricetus; D008853:Microscopy / Q000295:instrumentation; D008854:Microscopy, Electron
chemical_list: D002241:Carbohydrates; D037102:Lectins; D003165:Complement System Proteins