[UP-PCR diversity analysis of Fusarium population isolated from greenhouse melon soils].
A total of 112 Fusarium isolates were obtained from 36 soil samples collected from the greenhouse melon fields of Liaoning Province, among which, 11 species were identified by traditional morphological classification and rDNA sequence analysis. Universally Primed PCR (UP-PCR) was conducted to analyze the 25 strains of test Fusarium isolates and 3 strains of positive control Fusarium isolates. The results indicated that a total of 73 bands appeared after amplification by using 6 primers, and 66 bands (90.4%) were polymorphic. The isolates were clustered into eight groups at the similarity of 0.736 by cluster analysis, among which, 14 isolates were clustered into one group. It was concluded that UP-PCR could present the genetic relationship and difference among Fusarium strains, being able to be used as an assistant method for Fusarium classification.
Zhao
Bai-Xia
BX
Ministry of Agriculture Key Laboratory of Northern Crop Immunology, College of Plant Protection, Shenyang Agricultural University, Shenyang 110161, China. zhaobaixia1979@126.com
Gao
Zeng-Gui
ZG
Zhuang
Jing-Hua
JH
Zhang
Xiao-Fei
XF
Zhao
Hui
H
D044822:Biodiversity; D002681:China; D019662:Cucurbitaceae / Q000254:growth & development*; D004271:DNA, Fungal / Q000032:analysis; D005670:Fusarium / Q000145:classification / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology
D004271:DNA, Fungal