[Efficient approach for potato viral pathogen sensitive diagnostic and identification].
Potato, one of the most widespread agricultural plants in Russia, is strongly affected by various pathogens of viral, bacterial, and fungal origin as well as by pests. Their simple and accurate diagnostics and identification sound rather important both for production of virus free planting material and to perform monitoring of the phytosanitary state of planting areas. Based on qualitative Fluorescent Amplification--based Specific Hybridization Polymerase Chain Reaction (FLASH-PCR) we have developed the diagnostic systems, which provided fast, careful, and with the minimum risk of contamination in the working zone by amplification products, detection of the major potato pathogens, i. e. A, Y, X, M, S potato viruses, potato leafroll virus, potato mop top virus, as well as potato spindle tuber viroids.
Riazantsev
D Iu
DIu
Zavriev
S K
SK
D009693:Nucleic Acid Hybridization; D010935:Plant Diseases / Q000821:virology*; D010942:Plant Viruses / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction; D011198:Solanum tuberosum / Q000821:virology*