Development and validation of an improved liquid chromatography-mass spectrometry method for the determination of pemetrexed in human plasma.
An improved liquid chromatography-mass spectrometry method for the determination of pemetrexed in human plasma was developed and validated using a simple quadrupole LC-MS and a new SPE cartridge (Plexa Bond Elut). The analysis was achieved with a C18 analytical column using a mobile phase consisting of formic acid/acetonitrile and isocratic flow for 7 min. The linear ranges (r(2)>0.99) were found from 5 to 5000 ng/mL. The lower limit of detection was 2.5 ng/mL. Within-day and between-day precisions were less than 7.2% and inaccuracy did not exceed 2.8%. This new method is suitable to support pharmacokinetic studies and drug monitoring.
Bobin-Dubigeon
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C
Department of Oncology Biology, CLCC René Gauducheau, Bd J Monod 44805 Nantes Saint Herblain Cedex, France. c-bobin@nantes.fnclcc.fr
Amiand
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MB
Herrenknecht
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C
Bard
J M
JM
D000970:Antineoplastic Agents / Q000097:blood*; D002853:Chromatography, Liquid / Q000379:methods*; D016903:Drug Monitoring / Q000379:methods; D005971:Glutamates / Q000097:blood*; D006147:Guanine / Q000031:analogs & derivatives* / Q000097:blood; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods*; D000068437:Pemetrexed; D012680:Sensitivity and Specificity
D000970:Antineoplastic Agents; D005971:Glutamates; D000068437:Pemetrexed; D006147:Guanine