title: Acute homing of bone marrow-derived mononuclear cells in intramyocardial vs. intracoronary transplantation.
abstract: OBJECTIVE
  Cell homing optimisation after transplantation is critical in myocardial infarction (MI) cell therapy.
  
  
  METHODS
  Eight pigs were randomized to receiving autologous purified (111)indium-labeled bone marrow mononuclear cells (BMMCs) (10(8) cells/2 ml) by intramyocardial (IM) (n=4) or by intracoronary (IC) (n=4) transplantation after 90 minutes occlusion of the CX-coronary artery. Dual isotope SPECT imaging was performed 2 and 24 hours postoperatively. Two animals were additionally analyzed on the sixth postoperative day. Tissue samples from the major organs were analyzed.
  
  
  RESULTS
  In SPECT imaging revealed that BMMCs administered using IM injection remained in the injured area. In contrast, minor proportion of IC transplanted cells remained in the myocardium, as most of the cells showed homing in the lungs. Analysis of the biopsies showed a seven-fold greater number of cells in the myocardium for the IM method and a 10-fold greater number of cells in the lungs in the IC group (p < 0.001).
  
  
  CONCLUSIONS
  In producing persistently high cell homing at the infarction site, the IM transplantation is superior to the IC transplantation. However, the IC administration might be more specific in targeting injured capillaries and epithelial cells within the infarcted myocardium.
authors: Department of Surgery, Oulu University Hospital, University of Oulu and Clinical Research Center, 90221, Oulu, Finland.
authors: Jussi
authors: J
authors: Mäkelä
authors: Vesa
authors: V
authors: Anttila
authors: Kari
authors: K
authors: Ylitalo
authors: Reijo
authors: R
authors: Takalo
authors: Siri
authors: S
authors: Lehtonen
authors: Timo
authors: T
authors: Mäkikallio
authors: Eija
authors: E
authors: Niemelä
authors: Sebastian
authors: S
authors: Dahlbacka
authors: Jonne
authors: J
authors: Tikkanen
authors: Kai
authors: K
authors: Kiviluoma
authors: Tatu
authors: T
authors: Juvonen
authors: Petri
authors: P
authors: Lehenkari
mesh_terms: D000818:Animals; D016026:Bone Marrow Transplantation / Q000379:methods*; D002470:Cell Survival / Q000187:drug effects; D018380:Hematopoietic Stem Cell Transplantation / Q000379:methods*; D006412:Hematopoietic Stem Cells / Q000502:physiology*; D007205:Indium Radioisotopes / Q000009:adverse effects; D007261:Infusions, Intra-Arterial; D007273:Injections, Intramuscular; D009203:Myocardial Infarction / Q000000981:diagnostic imaging / Q000601:surgery*; D011877:Radionuclide Imaging; D013552:Swine
chemical_list: D007205:Indium Radioisotopes