title: [Research on an improved Lowry method to determine content of protein in Sabin IPV].
abstract: OBJECTIVE
  To establish a method for the content determination of protein in Sabin IPV.
  
  
  METHODS
  Using lowry method combined with being precipitated by trichloroacetic acid to determine the content of protein in Sabin IPV. Changing different conditions to optimize the experiment to establish a improved lowry method. And the sample recovery test was also conducted.
  
  
  RESULTS
  The method can exclude the interference of free aminoacid, phenols and some other additives. The calibration curve was in good linearity of protein within the range of 2.5 microg/ml-40 Microg/ml, r = 0.9998. Under the best conditions, the mean recovery was 95.32%, the CV in a batch and between batches were both < 10%.
  
  
  CONCLUSIONS
  The method can be used to determine the micro content of protein in vaccines.
authors: Institute of Medical Biology, Chinese Academy of Medical Sciences, Pekin Union Medical College, Kunming 650118, China.
authors: Wei
authors: W
authors: Cai
authors: Ming-bo
authors: MB
authors: Sun
authors: Hong-bo
authors: HB
authors: Chen
authors: Chao
authors: C
authors: Hong
authors: Xiao-yan
authors: XY
authors: Song
authors: Lei
authors: L
authors: Yu
authors: Shu-de
authors: SD
authors: Jiang
authors: Guo-yang
authors: GY
authors: Liao
authors: Jing-si
authors: JS
authors: Yang
mesh_terms: D000596:Amino Acids / Q000378:metabolism; D002138:Calibration; D002623:Chemistry Techniques, Analytical / Q000379:methods*; D010636:Phenols / Q000737:chemistry; D011055:Poliovirus Vaccine, Oral / Q000737:chemistry*; D011506:Proteins / Q000032:analysis*; D014238:Trichloroacetic Acid / Q000737:chemistry
chemical_list: D000596:Amino Acids; D010636:Phenols; D011055:Poliovirus Vaccine, Oral; D011506:Proteins; D014238:Trichloroacetic Acid