title: [Effective inactivatian test of inactivated hepatitis A vaccine using integrated cell culture/strand-specific reverse transcriptase-polymerase chain reaction].
abstract: OBJECTIVE
  To establish an quick, sensitive and specific assay for effective inactivatian test of inactivated hepatitis A vaccine.
  
  
  METHODS
  effective inactivatian test of inactivated hepatitis A vaccine were carried out using integrated cell culture/strand-specific RT-PCR (ICC/strand-specific RT-PCR) assay compared with traditional ELISA and nest RT-PCR assay.
  
  
  RESULTS
  all the samples were infectious negative detecting by both ICC/ strand-specific RT-PCR and ELISA assay,while some samples appeared false positive detecting by nest RT-PCR.
  
  
  CONCLUSIONS
  ICC/strand-specific RT-PCR assay is a novel, rapid, sensitive and reliable method for effective inactivatian test of inactivated hepatitis A vaccine. Shorting detection period largely, this assay may be used as an alternative method for routine inactivated hepatitis A vaccines test.
authors: Chinese Academy of Medical Sciences and Peking Union Medical College, Kunming 650118, China.
authors: Jian
authors: J
authors: Zhou
authors: Guang
authors: G
authors: Ji
authors: Jia-na
authors: JN
authors: Wen
authors: Jia
authors: J
authors: Li
authors: Wei
authors: W
authors: Sheng
authors: Zhi-quan
authors: ZQ
authors: Guo
authors: Guo-yang
authors: GY
authors: Liao
authors: Shu-de
authors: SD
authors: Jiang
authors: Ming-bo
authors: MB
authors: Sun
mesh_terms: D000818:Animals; D001709:Biotechnology / Q000379:methods*; D018929:Cell Culture Techniques; D002478:Cells, Cultured; D004797:Enzyme-Linked Immunosorbent Assay; D022362:Hepatitis A Vaccines / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D012680:Sensitivity and Specificity; D015164:Vaccines, Inactivated / Q000378:metabolism*; D014765:Viral Vaccines / Q000378:metabolism*; D014776:Virus Cultivation / Q000379:methods; D038301:Virus Inactivation
chemical_list: D022362:Hepatitis A Vaccines; D015164:Vaccines, Inactivated; D014765:Viral Vaccines