Effect of synthetic vernix biofilms on barrier recovery of damaged mouse skin.
The aim of this work was to investigate whether topical application of synthetic biofilms supports and accelerates the recovery of the murine skin barrier, disrupted by sequential tape stripping. Therefore, various biofilms were applied topically on disrupted mouse skin to determine which formulation could improve barrier function, as was observed previously for the natural biofilm vernix caseosa (VC). The biofilms [i.e. particles (synthetic corneocytes) embedded in a synthetic lipid matrix] mimic closely the physicochemical properties and structure of VC. Various formulations were prepared using different particle:lipid ratios, particles with different initial water content and uncoated or lipid-coated particles. It was observed that application of all tested formulations improved the skin barrier recovery rate and reduced crust formation and epidermal hyperproliferation. However, only one of the biofilms [i.e. B1; composed of uncoated particles with 50% (w/w) initial water content and particle:lipid ratio of 2:1] mimicked the effects of native VC most closely. This indicates the importance of the presence of individual components, i.e. barrier lipids and water, as well as the ratio of these components. Consequently, these observations suggest the potential use of this biofilm treatment clinically.
Oudshoorn
Marion H M
MH
Department of Pharmaceutics, Utrecht Institute for Pharmaceutical Sciences (UIPS), Utrecht University, Utrecht, The Netherlands.
Rissmann
Robert
R
van der Coelen
Dennis
D
Hennink
Wim E
WE
Ponec
Maria
M
Bouwstra
Joke A
JA
D000818:Animals; D019540:Area Under Curve; D018441:Biofilms; D001706:Biopsy; D049109:Cell Proliferation; D000078404:Epidermal Cells; D006801:Humans; D008055:Lipids / Q000737:chemistry; D008297:Male; D051379:Mice; D012867:Skin / Q000187:drug effects* / Q000378:metabolism; D012879:Skin Physiological Phenomena; D013997:Time Factors; D014708:Vernix Caseosa / Q000378:metabolism*; D014867:Water / Q000737:chemistry
D008055:Lipids; D014867:Water