title: FGF23 is mainly synthesized by osteocytes in the regularly distributed osteocytic lacunar canalicular system established after physiological bone remodeling.
abstract: This study aimed to evaluate whether the immunolocalization of fibroblast growth factor (FGF) 23 and dentin matrix protein 1 (DMP1) is associated with the spatial regularity of the osteocyte lacunar canalicular system(s) (OLCS). Femora of 12-weeks-old male ICR mice were fixed with 4% paraformaldehyde, decalcified with a 10% EDTA solution and then embedded in paraffin. We have devised a triple staining procedure that combines silver impregnation, alkaline phosphatase (ALPase) immunohistochemistry and tartrate-resistant acid phosphatase (TRAPase) enzyme histochemistry on a single paraffin section. This procedure permitted the visualization of ALPase-positive plump osteoblasts and several TRAPase-positive osteoclasts on those bone matrices featuring irregularly arranged OLCS, and of ALPase-positive bone lining cells on the bone matrix displaying the well-arranged OLCS. As observations proceeded from the metaphysis toward the diaphysis, the endosteal cortical bone displayed narrower bands of calcein labeling, accompanied by increased regularity of the OLCS. This implies that the speed of bone deposition during bone remodeling would affect the regularity of the OLCS. While DMP1 was evenly localized in all regions of the cortical bones, FGF23 was more abundantly localized in osteocytes of cortical bones with regularly arranged OLCS. In cortical bones, the endosteal area featuring regular OLCS exhibited more intense FGF23 immunoreaction when compared to the periosteal region, which tended to display irregular OLCS. In summary, FGF23 appears to be synthesized principally by osteocytes in the regularly distributed OLCS that have been established after bone remodeling.
authors: Center for Transdisciplinary Research, Graduate School of Medical and Dental Sciences, Niigata University, Niigata, Japan.
authors: Sobhan
authors: S
authors: Ubaidus
authors: Minqi
authors: M
authors: Li
authors: Sara
authors: S
authors: Sultana
authors: Paulo Henrique Luiz
authors: PH
authors: de Freitas
authors: Kimimitsu
authors: K
authors: Oda
authors: Takeyasu
authors: T
authors: Maeda
authors: Ritsuo
authors: R
authors: Takagi
authors: Norio
authors: N
authors: Amizuka
mesh_terms: D000818:Animals; D016723:Bone Remodeling / Q000502:physiology*; D016326:Extracellular Matrix Proteins / Q000096:biosynthesis; D005269:Femur / Q000502:physiology*; D000089703:Fibroblast Growth Factor-23; D005346:Fibroblast Growth Factors / Q000096:biosynthesis*; D007150:Immunohistochemistry / Q000379:methods; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D010011:Osteocytes / Q000378:metabolism*
chemical_list: C513308:Dmp1 protein, mouse; D016326:Extracellular Matrix Proteins; C000717428:Fgf23 protein, mouse; D005346:Fibroblast Growth Factors; D000089703:Fibroblast Growth Factor-23