A microfluidic platform to synthesize a G-quadruplex binding ligand.
An aromatic triarylpyridine chromophore promotes pi-stacking interactions with the terminal G-tetrad in quadruplex DNA, stabilizing the structure and presenting a pathway towards cancer treatment by inhibition of telomerase. An interesting parent compound in this class is the dimethylamino functionalised 4'-aryl-2,6-bis(4-aminophenyl)pyridine. However, access to this compound using traditional batch synthetic methodology is limited, due to thermodynamic and kinetic constraints. A novel approach to the synthesis of this compound has been developed, involving dynamic thin films, overcoming a series of competing reactions, effectively controlling chemical reactivity and selectivity.
Smith
N M
NM
Centre for Strategic Nano-fabrication, School of Biomedical, Biomolecular and Chemical Sciences, Crawley, WA-6009, Australia.
Corry
B
B
Swaminathan Iyer
K
K
Norret
M
M
Raston
C L
CL
D001483:Base Sequence; D004247:DNA / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D031541:Fluorescence Resonance Energy Transfer; D054856:G-Quadruplexes; D008024:Ligands; D044085:Microfluidics / Q000295:instrumentation / Q000379:methods*; D008958:Models, Molecular; D008968:Molecular Conformation; D011725:Pyridines / Q000138:chemical synthesis* / Q000737:chemistry / Q000378:metabolism*
D008024:Ligands; D011725:Pyridines; D004247:DNA