[Detection of influenza viruses/avian influenza viruses and identification of virulence using a microarray].
OBJECTIVE
To establish the DNA microarray to detect influenza viruses and avian influenza viruses, and identify their virulence.


METHODS
Hemagglutinin (HA), neuramidinase (NA) and nucleoprotein(NP) genes were chosen simultaneously as targets for designing a microarray used for detection of viruses and identification virulence. The nucleic acid were amplified by single primer amplication (SPA). And then its specificity,sensitivity and reproducibility were evaluated.


RESULTS
The microarray was able to specially detect H1N1, H3N2, B influenza viruses and H5N1, H9N2 avian influenza viruses. Their limits were 8HAU, 16HAU, 32HAU, and 8HAU, 8HAU respectively. The limit for virulence was 32HAU. When samples were analyzed by both RT-PCR and microarray in parallel, the results agreed in 83.9% (47/56).


CONCLUSIONS
The microarray can detect and distinguish five tested viruses, and especially identify virulence. It not only supplies an assistant tool for clinical diagnosis and control of infectious disease, but also is valuable for controlling and preventing outbreak of avian influenza epidemic.
Jia
Fei
F
Life Science College, Liaoning University, Shenyang 110036, China.
Gao
Rong-bao
RB
Wang
Min
M
Guo
Yuan-ji
YJ
Wen
Le-ying
LY
Zhang
Ye
Y
Cheng
Yan-hui
YH
Shu
Yue-long
YL
Liu
Hong-sheng
HS
D000818:Animals; D001717:Birds; D006801:Humans; D009980:Influenza A virus / Q000235:genetics / Q000302:isolation & purification* / Q000472:pathogenicity; D005585:Influenza in Birds / Q000821:virology; D007251:Influenza, Human / Q000821:virology; D008828:Microbiological Techniques; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D009975:Orthomyxoviridae / Q000235:genetics / Q000302:isolation & purification* / Q000472:pathogenicity; D012367:RNA, Viral / Q000032:analysis*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012680:Sensitivity and Specificity; D014774:Virulence / Q000235:genetics*
D012367:RNA, Viral