title: Sodium metabisulfite modulation of potassium channels in pain-sensing dorsal root ganglion neurons.
abstract: The effects of sodium metabisulfite (SMB), a general food preservative, on potassium currents in rat dorsal root ganglion (DRG) neurons were investigated using the whole-cell patch-clamp technique. SMB increased the amplitudes of both transient outward potassium currents and delayed rectifier potassium current in concentration- and voltage-dependent manner. The transient outward potassium currents (TOCs) include a fast inactivating (A-current or IA) current and a slow inactivating (D-current or ID) current. SMB majorly increased IA, and ID was little affected. SMB did not affect the activation process of transient outward currents (TOCs), but the inactivation curve of TOCs was shifted to more positive potentials. The inactivation time constants of TOCs were also increased by SMB. For delayed rectifier potassium current (IK), SMB shifted the activation curve to hyperpolarizing direction. SMB differently affected TOCs and IK, its effects major on A-type K+ channels, which play a role in adjusting pain sensitivity in response to peripheral redox conditions. SMB did not increase TOCs and IK when adding DTT in pipette solution. These results suggested that SMB might oxidize potassium channels, which relate to adjusting pain sensitivity in pain-sensing DRG neurons.
authors: Shanghai Clinical Center for Endocrine and Metabolic Disease, Shanghai Institute of Endocrine and Metabolic Disease, Department of Endocrine and Metabolic Disease, Ruijin Hospital, Shanghai JiaoTong University School of Medicine, Shanghai 200025, China.
authors: Aifang
authors: A
authors: Nie
authors: Cailing
authors: C
authors: Wei
authors: Ziqiang
authors: Z
authors: Meng
mesh_terms: D000818:Animals; D005727:Ganglia, Spinal / Q000502:physiology*; D008564:Membrane Potentials / Q000187:drug effects; D009474:Neurons / Q000187:drug effects / Q000502:physiology*; D010146:Pain; D011188:Potassium / Q000378:metabolism; D015221:Potassium Channels / Q000187:drug effects* / Q000378:metabolism; D051381:Rats; D013447:Sulfites / Q000494:pharmacology*
chemical_list: D015221:Potassium Channels; D013447:Sulfites; C005200:sodium metabisulfite; D011188:Potassium