Isolation and characterization of Xenorhabdus nematophila transposon insertion mutants defective in lipase activity against Tween.
We identified Xenorhabdus nematophila transposon mutants with defects in lipase activity. One of the mutations, in yigL, a conserved gene of unknown function, resulted in attenuated virulence against Manduca sexta insects. We discuss possible connections between lipase production, YigL, and specific metabolic pathways.
Richards
Gregory R
GR
Department of Bacteriology, University of Wisconsin-Madison, Madison, Wisconsin, USA.
Vivas
Eugenio I
EI
Andersen
Aaron W
AW
Rivera-Santos
Delmarie
D
Gilmore
Sara
S
Suen
Garret
G
Goodrich-Blair
Heidi
H
D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics / Q000502:physiology*; D004251:DNA Transposable Elements / Q000235:genetics / Q000502:physiology*; D008049:Lipase / Q000235:genetics / Q000502:physiology*; D018402:Manduca / Q000382:microbiology*; D005810:Multigene Family / Q000235:genetics / Q000502:physiology; D016254:Mutagenesis, Insertional / Q000379:methods*; D016366:Open Reading Frames / Q000235:genetics / Q000502:physiology; D014774:Virulence / Q000235:genetics; D020593:Xenorhabdus / Q000201:enzymology* / Q000235:genetics / Q000472:pathogenicity*
D001426:Bacterial Proteins; D004251:DNA Transposable Elements; D008049:Lipase