[Immune function changes caused by contact dermatitis in mice after intracutaneous injection of trichloroethylene].
OBJECTIVE
To investigate the contact dermatitis of BALB/c mice exposed to Trichloroethylene (TCE) via back intradermal injection and observe the influence on immune function of BALB/c mice.


METHODS
BALB/c mice were exposed to TCE through intradermal injection. The organ coefficient of spleen and thymus were calculated. Lymphocytes viability and lymphocytes proliferation assay, Macrophage activation and NK cell activity assay were carried out; the content of TNF-alpha and IFN-gamma in the serum were measured.


RESULTS
The thymic organ coefficient of TCE group was significantly lower than that of the negative control group (P < 0.01). The lymphocytes viability and lymphocytes proliferation of TCE group were obviously higher than those of the negative control group (P < 0.01). Compared to negative control group, the response of macrophage activation and NK cell activity of the TCE group were enhanced markedly (P < 0.01). In TCE group the serous content of TNF-alpha was evidently higher than that of the negative control group (P < 0.01).


CONCLUSIONS
Trichloroethylene as an allergen can cause contact dermatitis whose mechanism was supposed to be a delayed-type hypersensitivity.
Wang
Yan
Y
Department of Toxicology, Public Health College, Harbin Medical University, Harbin 150086, China. wangyan782913@163.com
Li
Baixiang
B
Gao
Shuying
S
D000818:Animals; D003877:Dermatitis, Contact / Q000276:immunology*; D005260:Female; D006968:Hypersensitivity, Delayed / Q000276:immunology*; D007694:Killer Cells, Natural / Q000276:immunology; D008213:Lymphocyte Activation / Q000187:drug effects; D008264:Macrophages / Q000276:immunology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D013601:T-Lymphocytes / Q000276:immunology; D014241:Trichloroethylene / Q000633:toxicity*
D014241:Trichloroethylene