[Study on the optimal extaction of total flavonoids from Tagetes erecta by central composite design-response surface methodology].
OBJECTIVE
To optimize the process of extracting total flavonoids from Tagetes erecta.


METHODS
The influential factors were extraction temperature, ethanol concentration, reflux time and solvent volume fold. The evaluating indicator was the extraction rate of total flavonoids from Tagetes erecta. The central composite design-response surface methodology was used to optimize the process and the prediction was carried out.


RESULTS
The optimum conditions of extraction were 80% ethanol, 2.5 hours for reflux, 35 volume folds of solvent and 70 degrees C.


CONCLUSIONS
It shows that the optimum model is simple and highly predictive.
Jia
Chang-Ping
CP
Heilongjiang University of Chinese Medicine, Harbin 150040, China. jcp841006@yahoo.com.cn
Tian
Bao-Cheng
BC
Li
Yan-Bing
YB
Gao
Ying-Bo
YB
Yu
Bo-Meng
BM
Pan
Wei-Ling
WL
D000431:Ethanol / Q000737:chemistry; D005419:Flavonoids / Q000737:chemistry / Q000302:isolation & purification*; D035264:Flowers / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D012997:Solvents; D013056:Spectrophotometry, Ultraviolet; D031686:Tagetes / Q000737:chemistry*; D013678:Technology, Pharmaceutical / Q000379:methods*; D013696:Temperature; D013997:Time Factors
D005419:Flavonoids; D012997:Solvents; D000431:Ethanol