Nup211, the fission yeast homolog of Mlp1/Tpr, is involved in mRNA export.
Synthetic lethal mutants have been previously isolated in fission yeast Schizosaccharomyces pombe, which genetically interact with spmex67, in order to identify the genes involved in mRNA export. The nup211 gene was isolated by complementation of the growth defect in one of the synthetic lethal mutants, SLMex2, under synthetic lethal condition. We showed that Nup211, fission yeast homolog of Mlpl/Mlp2/Tpr, is essential for vegetative growth and Nup211-GFP proteins expressed at endogenous level are localized mainly in nuclear periphery. The accumulation of poly(A)(+) RNA in the nucleus is exhibited when expression of nup211 is repressed or over-expressed. These results suggest that the Nup211 protein plays a pivotal role of mRNA export in fission yeast.
Bae
Jin-Ah
JA
Department of Education, Sungshin Women's University, Seoul 136-742, Republic of Korea.
Moon
Donggerami
D
Yoon
Jin Ho
JH
D021581:Active Transport, Cell Nucleus; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism*; D020043:Genes, Essential; D005800:Genes, Fungal; D005816:Genetic Complementation Test; D028884:Karyopherins / Q000235:genetics / Q000378:metabolism*; D012331:RNA, Fungal / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism*; D012568:Schizosaccharomyces / Q000235:genetics / Q000502:physiology*
D005656:Fungal Proteins; D028884:Karyopherins; D012331:RNA, Fungal; D012333:RNA, Messenger