Molecular and biological characterization of natural variants of Citrus dwarfing viroid.
Citrus dwarfing viroid has been proposed as an agent to control tree size in high-density plantations. Thirty-three field isolates have been characterized, and the most frequent sequence/s have been identified. Five distinct variants were selected for biological characterization. Symptom expression analysis demonstrated a good correlation between leaf/stem symptoms and plant growth. The discriminating nucleotide sequence differences included two deletions and an insertion resulting in a reorganization of the base pairing of the terminal left loop, two (G42 --> A and C52 --> U) changes found in one of the variants, and as many as thirteen changes located in the right and left regions flanking the CCR.
Murcia
N
N
Departamento de Protección Vegetal y Biotecnología, Instituto Valenciano de Investigaciones Agrarias, Centro de Protección Vegetal y Biotecnología, Apartado oficial, 46113 Moncada, Valencia, Spain.
Bernad
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L
Serra
P
P
Bani Hashemian
S M
SM
Duran-Vila
Núria
N
D002957:Citrus / Q000254:growth & development / Q000821:virology*; D014644:Genetic Variation; D010935:Plant Diseases / Q000821:virology*; D018515:Plant Leaves / Q000254:growth & development / Q000821:virology; D018547:Plant Stems / Q000254:growth & development / Q000821:virology; D014772:Viroids / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity*