CpG oligodeoxynucleotides promote phospholipase D dependent phagolysosome maturation and intracellular mycobacterial killing in M. tuberculosis infected type II alveolar epithelial cells.
CpG oligodeoxynucleotides have been previously shown to enhance antimycobacterial response in human monocytes/macrophages. The present study reports evidences showing the capability of CpG oligodeoxynucleotides to induce (i) host phospholipase D (PLD) activation, (ii) PLD dependent reactive oxygen intermediate production, (iii) PLD dependent phagolysosome maturation and (iv) PLD dependent intracellular mycobacterial killing in type II alveolar epithelial cells. These are the first evidences showing that alveolar epithelial cells may represent efficient effecter cells during primary innate antimycobacterial immune response.
Greco
Emanuela
E
Department of Biology, University of Rome Tor Vergata, Rome, Italy.
Santucci
Marilina Benedetta
MB
Quintiliani
Gianluca
G
Papi
Massimiliano
M
De Spirito
Marco
M
Fraziano
Maurizio
M
D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D045744:Cell Line, Tumor; D006801:Humans; D009169:Mycobacterium tuberculosis / Q000276:immunology*; D009838:Oligodeoxyribonucleotides / Q000494:pharmacology*; D010588:Phagosomes / Q000187:drug effects / Q000201:enzymology / Q000382:microbiology; D010739:Phospholipase D / Q000096:biosynthesis / Q000187:drug effects*; D011650:Pulmonary Alveoli / Q000187:drug effects* / Q000276:immunology / Q000382:microbiology; D020545:Respiratory Mucosa / Q000187:drug effects* / Q000276:immunology / Q000382:microbiology
D000276:Adjuvants, Immunologic; C408982:CPG-oligonucleotide; D009838:Oligodeoxyribonucleotides; D010739:Phospholipase D