[Comparative quantification of plasma hnRNP B1 mRNA in non-small cell lung cancer patients by real-time PCR].
BACKGROUND
Circulating cell-free nucleic acids are known to be a noninvasive diagnostic tool for cancer detection. Heterogeneous nuclear ribonucleoprotein (hnRNP) B1, a nuclear core complex, is overexpressed in early stage lung cancer. We intended to evaluate the usefulness of plasma hnRNP B1 mRNA in differentiating non-small cell lung cancer (NSCLC) from other benign lung diseases, especially pulmonary tuberculosis, which is highly prevalent in Korea and often difficult to distinguish from lung cancer.


METHODS
Plasma RNA was extracted from 30 patients with NSCLC, 30 patients with benign lung diseases including pulmonary tuberculosis, and 10 healthy controls. Plasma hnRNP B1 mRNA was measured by TaqMan Gene Expression Assay (Applied Biosystems, USA), and pre-developed beta-actin (ACTB) mRNA was used for normalization. We analyzed the relative gene expression data using the delta-delta Ct method.


RESULTS
Plasma hnRPN B1 mRNA was measurable in 93.3% (28/30) of NSCLC patients. Normalized 2-DeltaDeltaCt of plasma hnRPN B1 mRNA was 62.2 (95%Cl, 6.4-210.1) in NSCLC patients and 2.7 (95%Cl, 0.5-13.6) in benign lung disease patients (P<0.001, Mann-Whitney U test).


CONCLUSIONS
Plasma hnRNP B1 mRNA was significantly increased in patients with lung cancer compared with that in patients with other benign lung diseases. Plasma hnRNP B1 mRNA may be useful as a potential marker for the detection of NSCLC.
Kim
Jeong Man
JM
Department of Laboratory Medicine, Dong-A University College of Medicine, Seo-Gu, Busan, Korea.
Hwang
Sang Hyun
SH
Song
Eun Ju
EJ
Lee
Sang Yull
SY
Kim
Yeong Dae
YD
Lee
Chang Hun
CH
Lee
Min Ki
MK
Chang
Chulhun L
CL
Lee
Eun Yup
EY
D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor; D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics*; D005260:Female; D034481:Heterogeneous-Nuclear Ribonucleoprotein Group A-B / Q000097:blood* / Q000235:genetics; D006801:Humans; D008171:Lung Diseases / Q000097:blood / Q000235:genetics; D008175:Lung Neoplasms / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000097:blood* / Q000378:metabolism
D014408:Biomarkers, Tumor; D034481:Heterogeneous-Nuclear Ribonucleoprotein Group A-B; D012333:RNA, Messenger; C439768:hnRNP A2