title: Structural basis for the sequence-specific RNA-recognition mechanism of human CUG-BP1 RRM3.
abstract: The CUG-binding protein 1 (CUG-BP1) is a member of the CUG-BP1 and ETR-like factors (CELF) family or the Bruno-like family and is involved in the control of splicing, translation and mRNA degradation. Several target RNA sequences of CUG-BP1 have been predicted, such as the CUG triplet repeat, the GU-rich sequences and the AU-rich element of nuclear pre-mRNAs and/or cytoplasmic mRNA. CUG-BP1 has three RNA-recognition motifs (RRMs), among which the third RRM (RRM3) can bind to the target RNAs on its own. In this study, we solved the solution structure of the CUG-BP1 RRM3 by hetero-nuclear NMR spectroscopy. The CUG-BP1 RRM3 exhibited a noncanonical RRM fold, with the four-stranded beta-sheet surface tightly associated with the N-terminal extension. Furthermore, we determined the solution structure of the CUG-BP1 RRM3 in the complex with (UG)(3) RNA, and discovered that the UGU trinucleotide is specifically recognized through extensive stacking interactions and hydrogen bonds within the pocket formed by the beta-sheet surface and the N-terminal extension. This study revealed the unique mechanism that enables the CUG-BP1 RRM3 to discriminate the short RNA segment from other sequences, thus providing the molecular basis for the comprehension of the role of the RRM3s in the CELF/Bruno-like family.
authors: RIKEN Systems and Structural Biology Center, Tsurumi, Japan.
authors: Kengo
authors: K
authors: Tsuda
authors: Kanako
authors: K
authors: Kuwasako
authors: Mari
authors: M
authors: Takahashi
authors: Tatsuhiko
authors: T
authors: Someya
authors: Makoto
authors: M
authors: Inoue
authors: Takaho
authors: T
authors: Terada
authors: Naohiro
authors: N
authors: Kobayashi
authors: Mikako
authors: M
authors: Shirouzu
authors: Takanori
authors: T
authors: Kigawa
authors: Akiko
authors: A
authors: Tanaka
authors: Sumio
authors: S
authors: Sugano
authors: Peter
authors: P
authors: Güntert
authors: Yutaka
authors: Y
authors: Muto
authors: Shigeyuki
authors: S
authors: Yokoyama
mesh_terms: D020816:Amino Acid Motifs; D001483:Base Sequence; D001665:Binding Sites; D000067879:CELF1 Protein; D002151:Calorimetry; D006801:Humans; D008958:Models, Molecular; D008969:Molecular Sequence Data; D019906:Nuclear Magnetic Resonance, Biomolecular; D011485:Protein Binding; D012313:RNA / Q000737:chemistry*; D016601:RNA-Binding Proteins / Q000737:chemistry*; D012091:Repetitive Sequences, Nucleic Acid; D017386:Sequence Homology, Amino Acid
chemical_list: D000067879:CELF1 Protein; C102843:CELF1 protein, human; D016601:RNA-Binding Proteins; D012313:RNA