Optimization and production of a biosurfactant from the sponge-associated marine fungus Aspergillus ustus MSF3.
Marine endosymbiotic fungi Aspergillus ustus (MSF3) which produce high yield of biosurfactant was isolated from the marine sponge Fasciospongia cavernosa collected from the peninsular coast of India. Maximum production of biosurfactant was obtained in Sabouraud dextrose broth. The optimized bioprocess conditions for the maximum production was pH 7.0, temperature 20 degrees C, salt concentration 3%, glucose and yeast extract as carbon source and nitrogen sources respectively. The response surface methodology based analysis of carbon and nitrogen ratio revealed that the carbon source can increase the biosurfactant yield. The biosurfactant produced by MSF3 was partially characterized as glycolipoprotein based on the estimation of macromolecules and TLC analysis. The partially purified biosurfactant showed broad spectrum of antimicrobial activity. The strain MSF3 can be used for the microbially enhanced oil recovery process.
Kiran
G Seghal
GS
Department of Microbiology, Bharathidasan University, Tiruchirappalli, India.
Hema
T A
TA
Gandhimathi
R
R
Selvin
Joseph
J
Thomas
T Anto
TA
Rajeetha Ravji
T
T
Natarajaseenivasan
K
K
D000818:Animals; D000890:Anti-Infective Agents / Q000494:pharmacology; D001230:Aspergillus / Q000737:chemistry* / Q000302:isolation & purification; D002244:Carbon / Q000494:pharmacology; D002412:Cations / Q000494:pharmacology; D003247:Conservation of Natural Resources; D003470:Culture Media; D006863:Hydrogen-Ion Concentration / Q000187:drug effects; D008826:Microbial Sensitivity Tests; D009584:Nitrogen / Q000494:pharmacology; D009821:Oils / Q000032:analysis; D011161:Porifera / Q000382:microbiology*; D012965:Sodium Chloride / Q000494:pharmacology; D013501:Surface-Active Agents / Q000032:analysis / Q000138:chemical synthesis* / Q000737:chemistry; D013696:Temperature; D013997:Time Factors
D000890:Anti-Infective Agents; D002412:Cations; D003470:Culture Media; D009821:Oils; D013501:Surface-Active Agents; D012965:Sodium Chloride; D002244:Carbon; D009584:Nitrogen