Coiled-coil interactions are required for post-Golgi R-SNARE trafficking.
The sorting of post-Golgi R-SNAREs (vesicle-associated membrane protein (VAMP)1, 2, 3, 4, 7 and 8) is still poorly understood. To address this, we developed a system to investigate their localization, trafficking and cell-surface levels. Here, we show that the distribution and internalization of VAMPs 3 and 8 are determined solely through a new conserved mechanism that uses coiled-coil interactions, and that VAMP4 does not require these interactions for its trafficking. We propose that VAMPs 3 and 8 are trafficked while in a complex with Q-SNAREs. We also show that the dileucine motif of VAMP4 is required for both its internalization and retrieval to the trans-Golgi network. However, when the dileucine motif is mutated, the construct can still be internalized potentially through coiled-coil interactions with Q-SNAREs.
Gordon
David E
DE
Department of Clinical Biochemistry, University of Cambridge, Cambridge, UK.
Mirza
Myriam
M
Sahlender
Daniela A
DA
Jakovleska
Jovana
J
Peden
Andrew A
AA
D000818:Animals; D006056:Golgi Apparatus / Q000378:metabolism*; D006801:Humans; D008954:Models, Biological; D011485:Protein Binding; D021381:Protein Transport / Q000502:physiology; D050682:Q-SNARE Proteins / Q000378:metabolism; D050683:R-SNARE Proteins / Q000737:chemistry / Q000378:metabolism*; D021601:trans-Golgi Network / Q000378:metabolism
D050682:Q-SNARE Proteins; D050683:R-SNARE Proteins