Rhizobium mesosinicum sp. nov., isolated from root nodules of three different legumes.
Thirteen novel strains were isolated from root nodules of three different leguminous plants of the genera Albizia, Kummerowia and Dalbergia grown in China. Cells of these strains were Gram-negative, strictly aerobic, non-spore-forming, motile rods. Phylogenetic analysis of 16S rRNA gene sequences revealed that they belong to the genus Rhizobium. A representative strain, CCBAU 25010T, showed 98.8% 16S rRNA gene sequence similarity to its closest phylogenetic relative, Rhizobium sullae IS123T. The 16S-23S intergenic spacer (ITS) sequence of CCBAU 25010T shared 91.5 and 87.2% sequence similarity, respectively, with those of Rhizobium etli CFN 42T and Rhizobium leguminosarum LMG 14904T. Analysis of the sequences of the housekeeping genes atpD and recA was in agreement with the results of ITS sequence analysis. The nodC gene sequence of CCBAU 25010T was identical to that of Rhizobium tropici CFN 299. DNA-DNA hybridization values for strain CCBAU 25010T ranged from 20.7% (with Rhizobium mongolense USDA 1844T) to 34.4% (with R. leguminosarum USDA 2370T). Cell protein SDS-PAGE, BOX-PCR and several phenotypic characteristics, such as use of sole carbon sources and antibiotic resistance, could differentiate the novel strains from defined Rhizobium species. We therefore propose that the novel strains reported in this study form a novel species, Rhizobium mesosinicum sp. nov., with the type strain CCBAU 25010T (=LMG 24135T =JCM 14777T).
Lin
Dong Xu
DX
State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing 100193, PR China.
Chen
Wen Feng
WF
Wang
Feng Qin
FQ
Hu
Dong
D
Wang
En Tao
ET
Sui
Xin Hua
XH
Chen
Wen Xin
WX
D000332:Aerobiosis; D001426:Bacterial Proteins / Q000235:genetics; D015373:Bacterial Typing Techniques; D002681:China; D016000:Cluster Analysis; D016172:DNA Fingerprinting / Q000379:methods; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000737:chemistry / Q000235:genetics; D007887:Fabaceae / Q000382:microbiology*; D008124:Locomotion; D008969:Molecular Sequence Data; D017351:N-Acetylglucosaminyltransferases / Q000235:genetics; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D018517:Plant Roots / Q000382:microbiology*; D020543:Proteome / Q000032:analysis; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D011938:Rec A Recombinases / Q000235:genetics; D012231:Rhizobium / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D017422:Sequence Analysis, DNA; D014157:Transcription Factors / Q000235:genetics
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D021903:DNA, Ribosomal Spacer; D020543:Proteome; D012336:RNA, Ribosomal, 16S; D014157:Transcription Factors; D017351:N-Acetylglucosaminyltransferases; C060771:NodC protein, Rhizobiales; D011938:Rec A Recombinases