The lipophilicity indices of flavonoids estimated by reversed-phase liquid chromatography using different computation methods.
The chromatographic behavior of some flavonoids was established by RP-HPLC on RP-18 (LiChroCART, LiChrosphere RP-18e), RP-8 (Zorbax, Eclipse XDB-C8), CN (Säulentechnik, LiChrosphere CN100) columns. The mobile phases were mixtures of methanol-water in different volume proportions from 70 to 80% v/v for RP-18 and RP-8, while for the CN column the proportions were between 66 and 70% v/v. The lipophilicity was expressed through different lipophilicity descriptors such as mean of k (mk), mean of log k (mlog k), log k(W), S, f() and scores of k and log k corresponding to the first principal component. The experimental lipophilicity indices are directly correlated with the computed values, via computer software and internet module, at a high analytical level. Furthermore, the results obtained applying principal component analysis to k and/or log k values allow the prediction and explanation of the interaction involved in the retention mechanism which takes place between the compounds and the employed stationary phases during the development.
Briciu
Rodica Domnica
RD
Faculty of Chemistry and Chemical Engineering, Babes-Bolyai University, Cluj Napoca, Romania.
Kot-Wasik
Agata
A
Namiesnik
Jacek
J
Sârbu
Costel
C
D000465:Algorithms; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D005419:Flavonoids / Q000032:analysis*; D006801:Humans; D000432:Methanol / Q000737:chemistry; D025341:Principal Component Analysis / Q000379:methods*; D012984:Software; D014867:Water / Q000737:chemistry
D005419:Flavonoids; D014867:Water; D000432:Methanol