title: Phase contrast microradiography of mouse lung using synchrotron X-ray: correlation with optical microscopy.
abstract: OBJECTIVE
  The purpose of this study is to evaluate the feasibility of phase contrast X-ray microtomography and microradiography, using a polychromatic synchrotron X-ray, for analysis of the mouse lung microstructure.
  
  
  METHODS
  Normal mice were used for experiments. Some of the mouse lungs were prepared by the lung fixation-inflation method. The resulting sponge-like inflated lung samples were used for microtomography. The remaining mouse lungs were cut into 10 microm sections and were used for microradiography and optical microscopic correlation. The experiments on mouse lung samples were performed at the 7B2 beamline of the Pohang Light Source in Korea.
  
  
  RESULTS
  Phase contrast X-ray microtomography of inflated lung samples showed individual alveolar structure on 3-D reconstruction. Phase contrast microradiographs of thin lung samples showed microstructure of lung, such as alveoli and bronchioles, and were well correlated with optical microscopic images.
  
  
  CONCLUSIONS
  The results indicate that the phase contrast X-ray microtomography and microradiography using polychromatic synchrotron X-ray is feasible for evaluation of microstructure of the lung.
authors: Department of Radiology, Korea University Guro Hospital, 97 Guro-dong, Guro-gu, Seoul, Korea.
authors: Hwan Seok
authors: HS
authors: Yong
authors: Eun-Young
authors: EY
authors: Kang
authors: Yoon Kyung
authors: YK
authors: Kim
authors: Ok Hee
authors: OH
authors: Woo
authors: Bong Kyoung
authors: BK
authors: Shin
authors: Chil Hwan
authors: CH
authors: Oh
authors: Jung Ho
authors: JH
authors: Je
authors: Heon
authors: H
authors: Han
authors: Jae Seung
authors: JS
authors: Seo
mesh_terms: D000818:Animals; D066298:In Vitro Techniques; D008168:Lung / Q000166:cytology* / Q000000981:diagnostic imaging*; D051379:Mice; D008853:Microscopy / Q000379:methods*; D008858:Microscopy, Phase-Contrast; D055114:X-Ray Microtomography / Q000379:methods*