Preparative separation and purification of liensinine, isoliensinine and neferine from seed embryo of Nelumbo nucifera GAERTN using high-speed counter-current chromatography.
A preparative high-speed counter-current chromatography method for separation and purification of liensinine, isoliensinine and neferine from seed embryo of Nelumbo nucifera GAERTN was successfully established by using n-hexane-ethyl acetate-methanol-water (5:8:4:5, v/v, containing 0.5% NH(4)OH) as the two-phase solvent system. From 200 mg of crude extract, 18.4 mg of liensinine, 19.6 mg of isoliensinine and 58.4 mg of neferine were obtained with the purity of 96.8, 95.9, and 98.6%, respectively. The identification of the three alkaloids was performed with (1)H NMR and (13)C NMR.
Liu
Shao
S
Department of Pharmacy, Xiangya hospital, Central South University, Changsha, PR China.
Wang
Bin
B
Li
Xin-Zhong
XZ
Qi
Long-feng
LF
Liang
Yi-Zeng
YZ
D044182:Benzylisoquinolines / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid; D003377:Countercurrent Distribution / Q000295:instrumentation / Q000379:methods*; D007546:Isoquinolines / Q000302:isolation & purification*; D009682:Magnetic Resonance Spectroscopy; D031653:Nelumbo / Q000737:chemistry*; D010636:Phenols / Q000302:isolation & purification*; D010936:Plant Extracts / Q000302:isolation & purification*; D012639:Seeds / Q000737:chemistry*
D044182:Benzylisoquinolines; D007546:Isoquinolines; D010636:Phenols; D010936:Plant Extracts; C499779:isoliensinine; C057222:neferine; C080095:liensinine