title: Genomic sequence of a Japanese encephalitis virus isolate from southern China.
abstract: We determined the complete nucleotide sequence of a Japanese encephalitis virus (JEV) isolate (designated SH17M-2007) from a pool of Culex tritaeniorhynchus collected in southern China in 2007. The genome consisted of 10,965 nucleotides and included a single open reading frame (10,296 nucleotides) that encodes a 3,432-amino-acid polyprotein. The SH17M-2007 had 97.3 to 98.4% nucleotide identity with two Korean strains (KV1899, K94P05) and two Japanese strains (Ishikawa, JEV/sw/Mie/40/2004), but only 88.8% identity with the Chinese vaccine strain SA14-14-2. Five unique amino acid substitutions including one in the envelope (E) protein (Glu(E-306)-Lys) were found in the SH17M-2007 strain. Phylogenetic relationships based on the full-length nucleotide sequences were similar to those based on the E gene.
authors: Beijing Institute of Microbiology and Epidemiology, People's Republic of China. Zhang_jiusong@yahoo.com.cn
authors: Jiu-Song
authors: JS
authors: Zhang
authors: Qiu-Min
authors: QM
authors: Zhao
authors: Pan-He
authors: PH
authors: Zhang
authors: Na
authors: N
authors: Jia
authors: Wu-Chun
authors: WC
authors: Cao
mesh_terms: D000818:Animals; D001483:Base Sequence; D002681:China; D003465:Culex / Q000821:virology; D004664:Encephalitis Virus, Japanese / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D016679:Genome, Viral; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010802:Phylogeny