Renal ischemia/reperfusion injury in rats is attenuated by a synthetic glycine derivative.
Renal ischemia/reperfusion is a common cause of acute renal failure. Glycine is an effective anti-inflammatory, cytoprotective agent and is reported to have a beneficial effect against ischemia/reperfusion injury in various organs. Previous research notes that free radicals and inflammatory leukocytes both play important roles in the pathogenesis of renal ischemia/reperfusion injury. To develop new therapeutic agents against renal ischemia/reperfusion injury, we sought to link an antioxidant moiety (nitronyl nitroxide) to glycine in the hope that the resulting glycine-nitronyl nitroxide conjugate (GNN) would provide a synergetic protection against renal ischemia/reperfusion injury. In this manuscript, we report the synthesis and biological evaluation of the GNN conjugate. The biological activity of the GNN conjugate was evaluated in an in vivo rat model of renal ischemia/reperfusion induced injury and oxidative change. Since the GNN conjugate markedly reduced elevated levels of tissue lipid peroxidation and attenuated renal dysfunction in rats subjected to renal ischemia/reperfusion, it might be possible to develop the GNN conjugate into a potential therapeutic agent against renal ischemia/reperfusion injury.
Bi
Wei
W
Department of Surgery, Second Hospital of HeBei Medical University, Shijiazhuang 050000, PR China.
Wang
Fengan
F
Bi
Yue
Y
Wang
Tianyang
T
Xue
Ping
P
Zhang
Yanrong
Y
Gao
Xiang
X
Liu
Sanguang
S
Wang
Zhibo
Z
Li
Meng
M
Baudy-Floc'h
Michèle
M
Robinson
Sara C
SC
Ngerebara
Nathaniel
N
Bi
Lanrong
L
D000109:Acetylcholine / Q000494:pharmacology; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000737:chemistry; D001806:Blood Urea Nitrogen; D016166:Free Radical Scavengers / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology*; D005978:Glutathione / Q000378:metabolism; D005998:Glycine / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000494:pharmacology* / Q000627:therapeutic use; D066298:In Vitro Techniques; D007668:Kidney / Q000098:blood supply / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D009589:Nitrogen Oxides / Q000737:chemistry; D018384:Oxidative Stress / Q000187:drug effects; D016716:PC12 Cells; D009195:Peroxidase / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000188:drug therapy* / Q000378:metabolism / Q000503:physiopathology; D014664:Vasodilation / Q000187:drug effects
D000893:Anti-Inflammatory Agents; D016166:Free Radical Scavengers; D009589:Nitrogen Oxides; D008315:Malondialdehyde; D009195:Peroxidase; D005978:Glutathione; C039900:nitroxyl; D000109:Acetylcholine; D005998:Glycine