Fabrication and physical-chemical characterisation of polyelectrolyte microparticles: platform for controlled release of bioactives.
Porous CaCO(3) microparticles were fabricated by colloidal crystallization. Two oppositely charged polyelectrolytes, poly (styrene sulfonate, PSS) and poly (allylamine hydrochloride, PAH) were adsorbed layer-by-layer on the CaCO(3) templates. Polyelectrolyte microcapsules were then obtained by removing the CaCO(3) core. Scanning electron microscopy (SEM), energy-dispersion X-ray analysis (EDX), laser diffraction particle sizing and Raman spectroscopy were employed to characterize the physico-chemical properties of the constructed microcapsules. In vitro drug release studies were conducted using the model water-soluble drug Rhodamine B. Factors such as the number of polyelectrolyte layers and pH were investigated. SEM micrographs revealed uniform CaCO(3) microparticles, nearly spherical in shape with pronounced surface roughness, and highly developed interior porous structure. The surface of polyelectrolyte coated particles became rougher than the initial CaCO(3) microparticles. The acquired SEM micrographs of the (PSS/PAH)(n) microcapsules indicated that the number of layers affected the morphology of the microcapsules. The (PSS/PAH)(3) microcapsules revealed a very porous network with many holes resembling the initial morphology of CaCO(3) microparticles. Raman spectra showed peaks at 1125 cm(-1) (S=O bond) and 1600 cm(-1) (aromatic ring stretching) which represented the PSS molecule. The thickness of each layer was about 10 to 20 nm and it can be tailored to such nanometer level by controlling the number of adsorbed layers. The in vitro release of Rhodamine B was dependent on both the number of wall bilayers as well as the pH of the release media. These systems provide an opportunity for the development of controlled release dosage forms with greater effectiveness in the treatment of chronic conditions.
Sun
Yuhui
Y
Drug Delivery Research Unit (DDRU), School of Pharmacy, Faculty of Medical and Health Sciences, University of Auckland, Auckland, New Zealand.
Travas-Sejdic
Jadranka
J
Wen
Jingyuan
J
Alany
Raid G
RG
D002119:Calcium Carbonate / Q000737:chemistry*; D002214:Capsules; D003102:Colloids; D003460:Crystallization; D003692:Delayed-Action Preparations; D004573:Electrolytes / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D008855:Microscopy, Electron, Scanning; D011073:Polyamines / Q000737:chemistry*; D011137:Polystyrenes / Q000737:chemistry*; D016062:Porosity; D012235:Rhodamines / Q000737:chemistry*; D013059:Spectrum Analysis, Raman
D002214:Capsules; D003102:Colloids; D003692:Delayed-Action Preparations; D004573:Electrolytes; D011073:Polyamines; D011137:Polystyrenes; D012235:Rhodamines; C063994:polyallylamine; C003321:polystyrene sulfonic acid; D002119:Calcium Carbonate; C029773:rhodamine B