Characterization and anti-tumor activities of sulfated polysaccharide SRBPS2a obtained from defatted rice bran.
A novel chemically sulfated polysaccharide SRBPS2a with potent anti-tumor activity was derived from defatted rice bran by chlorosulfonic acid-pyridine (CSA-Pyr) method. The average molecular weight of SRBPS2a was 3.5 x 10(5) Da and the degree of sulfation (DS) was 1.29. The Fourier-transform infrared spectra (FT-IR) and 13C NMR spectroscopy analysis revealed that SRBPS2a was mainly consist of beta-(1-->3)-D-galactopyranosyl residues, the sulfate substitution site was on C-2 and C-4 while the side chains were cut off during the sulfated reaction. Furthermore, SRBPS2a exhibited evident growth inhibition on mouse mammary tumor EMT-6 cells both in vitro and in vivo.
Wang
Li
L
State Key Laboratory of Food Science and Technology, Jiangnan University, Lihu Road 1800, Wuxi 214122, China.
Huang
Hongyu
H
Wei
Yunyu
Y
Li
Xiaoxuan
X
Chen
Zhengxing
Z
D000818:Animals; D000970:Antineoplastic Agents / Q000737:chemistry* / Q000494:pharmacology* / Q000627:therapeutic use; D001943:Breast Neoplasms / Q000188:drug therapy; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D005260:Female; D009682:Magnetic Resonance Spectroscopy; D051379:Mice; D008970:Molecular Weight; D012275:Oryza / Q000033:anatomy & histology* / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry* / Q000494:pharmacology* / Q000627:therapeutic use; D017550:Spectroscopy, Fourier Transform Infrared; D013329:Structure-Activity Relationship; D013431:Sulfates / Q000737:chemistry*
D000970:Antineoplastic Agents; D011134:Polysaccharides; D013431:Sulfates