[In vitro study of directional inducible differentiation of inner ear hair cells from human adipose-derived mesenchymal stem cells].
OBJECTIVE
To explore the feasibility of directionally inducing human adipose-derived mesenchymal stem cells (hAD-MSCs) towards inner ear hair cells.


METHODS
Mesenchymal stem cells from human adipose tissue were isolated, purified and cultured in vitro. hAD-MSCs were induced to neural stem/progenitor-like cell, and then co-cultured with embryonic chick otic vesicle cells. Processed hAD-MSCs were tested by immunostaining to ascertain whether they expressed characteristic hair cell markers.


RESULTS
Morphologically, hAD-MSCs were induced to differentiate into neural stem/progenitor cells and expressed specific neural markers. After being co-cultured with embryonic chick otic vesicle cells, hAD-MSCs expressed specific surface markers of inner ear hair cells.


CONCLUSIONS
hAD-MSCs can be directionally induced to differentiate towards hair cell-like cells in vitro.
Yuan
Xian-dao
XD
Department of Otorhinolaryngoogy, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100730, China.
Yan
Xi
X
Yang
Hua
H
Gao
Zhi-qiang
ZQ
Li
Kang-hua
KH
Han
Qin
Q
Lu
Shan
S
Chen
Xiao-wei
XW
Jiang
Hong
H
Zhao
Chun-hua
CH
D000273:Adipose Tissue / Q000166:cytology; D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods*; D002454:Cell Differentiation; D002478:Cells, Cultured; D002642:Chick Embryo; D018920:Coculture Techniques; D006199:Hair Cells, Auditory, Inner / Q000166:cytology*; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology*