title: Tryptophan auxotrophs were obtained by random transposon insertions in the Methanococcus maripaludis tryptophan operon.
abstract: Methanococcus maripaludis is an anaerobic, methane-producing archaeon that utilizes H(2) or formate for the reduction of CO(2) to methane. Tryptophan auxotrophs were constructed by in vitro insertions of the Tn5 transposon into the tryptophan operon, followed by transformation into M. maripaludis. This method could serve for rapid insertions into large cloned DNA regions.
authors: Iris
authors: I
authors: Porat
authors: William B
authors: WB
authors: Whitman
mesh_terms: D052818:Autotrophic Processes; D004251:DNA Transposable Elements; D017017:Methanococcus / Q000235:genetics* / Q000378:metabolism*; D016254:Mutagenesis, Insertional / Q000379:methods*; D009876:Operon; D014364:Tryptophan / Q000378:metabolism*
chemical_list: D004251:DNA Transposable Elements; D014364:Tryptophan