title: Prolactin suppresses malonyl-CoA concentration in human adipose tissue.
abstract: Prolactin is best known for its involvement in lactation, where it regulates mechanisms that supply nutrients for milk production. In individuals with pathological hyperprolactinemia, glucose and fat homeostasis have been reported to be negatively influenced. It is not previously known, however, whether prolactin regulates lipogenesis in human adipose tissue. The aim of this study was to investigate the effect of prolactin on lipogenesis in human adipose tissue in vitro. Prolactin decreased the concentration of malonyl-CoA, the product of the first committed step in lipogenesis, to 77+/-6% compared to control 100+/-5% (p=0.022) in cultured human adipose tissue. In addition, prolactin was found to decrease glucose transporter 4 ( GLUT4) mRNA expression, which may cause decreased glucose uptake. In conclusion, we propose that prolactin decreases lipogenesis in human adipose tissue as a consequence of suppressed malonyl-CoA concentration in parallel with decreased GLUT-4 expression. In the lactating woman, this regulation in adipose tissue may enhance the provision of nutrients for the infant instead of nutrients being stored in adipose tissue. In hyperprolactinemic individuals, a suppressed lipogenesis could contribute to an insulin resistant state with consequences for the health.
authors: Institute of Neuroscience and Physiology, Sahlgrenska Academy at University of Gothenburg, Göteborg, Sweden. louise.nilsson@gu.se
authors: L A
authors: LA
authors: Nilsson
authors: C
authors: C
authors: Roepstorff
authors: B
authors: B
authors: Kiens
authors: H
authors: H
authors: Billig
authors: C
authors: C
authors: Ling
mesh_terms: D000103:Acetyl-CoA Carboxylase / Q000235:genetics / Q000378:metabolism; D000273:Adipose Tissue / Q000201:enzymology / Q000378:metabolism*; D000328:Adult; D005260:Female; D051275:Glucose Transporter Type 4 / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D015151:Immunoblotting; D050155:Lipogenesis / Q000502:physiology*; D008316:Malonyl Coenzyme A / Q000378:metabolism*; D008875:Middle Aged; D010766:Phosphorylation / Q000502:physiology; D011388:Prolactin / Q000378:metabolism*; D012313:RNA; D054839:Retinol-Binding Proteins, Plasma / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D051275:Glucose Transporter Type 4; C101326:RBP4 protein, human; D054839:Retinol-Binding Proteins, Plasma; C495692:SLC2A4 protein, human; D008316:Malonyl Coenzyme A; D012313:RNA; D011388:Prolactin; D000103:Acetyl-CoA Carboxylase