Effect of topically applied sphingomyelin-based liposomes on the ceramide level in a three-dimensional cultured human skin model.
Sphingomyelin-based liposomes were prepared and applied to the stratum corneum side or basal layer side of a three-dimensional (3D) cultured human skin model, and the increase in the type II ceramide (ceramide II) content of the cultured skin model was evaluated. The sphingomyelin-based liposomes were prepared by a high-pressure emulsification method, and the obtained liposomes were characterized; the particle diameter and zeta potential of the liposomes were 155.3 nm and -11.4 mV, respectively. Their spherical shape and lamella structure were observed by transmission electron microscopy. The sphingomyelin-based liposomes or saline were applied to the cultured skin model, and ceramide II was extracted from the skin model. The extracted ceramide II was separated by high-performance thin-layer chromatography and quantified by a densitometer. The amount of ceramide II in the cultured skin model was significantly increased by the application of the sphingomyelin-based liposomes, compared with the nonapplication group. Thus, sphingomyelin-based liposomes are useful for enriching the ceramide level in 3D cultured skin models.
Tokudome
Yoshihiro
Y
Faculty of Pharmaceutical Sciences, Josai University, Saitama, Japan.
Uchida
Raina
R
Yokote
Takeshi
T
Todo
Hiroaki
H
Hada
Nobuko
N
Kon
Tatsuhiko
T
Yasuda
Junko
J
Hayashi
Hidenori
H
Hashimoto
Fumie
F
Sugibayashi
Kenji
K
D000287:Administration, Topical; D002518:Ceramides / Q000032:analysis* / Q000737:chemistry; D002855:Chromatography, Thin Layer; D004817:Epidermis / Q000737:chemistry; D006801:Humans; D008081:Liposomes / Q000032:analysis / Q000737:chemistry*; D046529:Microscopy, Electron, Transmission; D012867:Skin / Q000737:chemistry; D013109:Sphingomyelins / Q000032:analysis
D002518:Ceramides; D008081:Liposomes; D013109:Sphingomyelins