title: Gene-specific silencing induced by parallel complementary RNA in Pseudomonas aeruginosa.
abstract: To investigate whether parallel complementary RNA (pRNA) could induce gene-specific silencing in Pseudomonas aeruginosa, pRNA of the mexA gene was expressed in it. Compared to the control strains, the strain expressing pRNA of mexA showed a 50% decrease in minimum inhibitory concentrations (MICs) of several antimicrobial agents and a twofold increase in the initial accumulation rate of ethidium bromide, all of which are substrates of the MexAB-OprM efflux pump. These results suggest that gene-specific silencing was induced by pRNA. This is the first time that such a route for gene silencing has been reported in a bacterium other than Escherichia coli. Gene-specific silencing induced by pRNA may be useful as a novel biotechnology tool for gene regulation in prokaryotes.
authors: Institute of Medicinal Biotechnology, Chinese Academy of Medical Science and Peking Union Medical College, Beijing, 100050, China.
authors: Yi Shuang
authors: YS
authors: Liu
authors: Yue Qin
authors: YQ
authors: Zhang
authors: Li Xia
authors: LX
authors: Yang
authors: Tian Jue
authors: TJ
authors: Yao
authors: Chun Ling
authors: CL
authors: Xiao
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001425:Bacterial Outer Membrane Proteins / Q000037:antagonists & inhibitors; D055785:Gene Knockdown Techniques / Q000379:methods*; D020868:Gene Silencing; D026901:Membrane Transport Proteins; D008826:Microbial Sensitivity Tests; D011550:Pseudomonas aeruginosa / Q000235:genetics*; D018075:RNA, Complementary / Q000235:genetics*
chemical_list: D000900:Anti-Bacterial Agents; D001425:Bacterial Outer Membrane Proteins; D026901:Membrane Transport Proteins; C118598:MexA protein, Pseudomonas aeruginosa; D018075:RNA, Complementary