Isoprostanes 8-iPF2alpha-III: risk markers of premature rupture of fetal membranes?
OBJECTIVE
Isoprostanes may serve as sensitive and specific markers of in vivo oxidative stress intensity. We wanted to determine, whether or not isoprostane concentration may be considered as a risk marker of premature rupture of fetal membranes (PROM).


METHODS
On the basis of the presence of PROM and gestational maturity, a total of 128 patients were divided into: (1) preterm PROM (pPROM) group; (2) PROM at term group; (3) control preterm (C1) group and (4) control at term (C2) group. The concentrations of 8-iPF(2alpha)-III were determined using the enzyme-linked immunosorbent assay method.


RESULTS
The mean free isoprostane concentrations, examined in amniotic fluid and maternal plasma in the PROM at term patients were significantly higher than in C2 individuals (p < 0.01). The mean concentrations of free 8-iPF(2alpha)-III measured in blood plasma from women in the C1 group were significantly lower than in patients from the pPROM, PROM at term and C2 groups (p < 0.001, p < 0.00001 and p < 0.00001, respectively).


CONCLUSIONS
The measurement of free isoprostane concentration in maternal plasma and amniotic fluid may be considered as a laboratory marker of a PROM-risk pregnancy.
Kwiatkowski
Sebastian
S
Department of Obstetrics and Gynecology, Pomeranian Medical University, Szczecin, Poland.
Torbé
Andrzej
A
Dołegowska
Barbara
B
Błogowski
Wojciech
W
Czajka
Ryszard
R
Chlubek
Dariusz
D
Rzepka
Rafał
R
D000328:Adult; D000653:Amniotic Fluid / Q000737:chemistry; D015415:Biomarkers / Q000097:blood; D016022:Case-Control Studies; D019411:Clinical Laboratory Techniques; D005260:Female; D005322:Premature Rupture of Fetal Membranes / Q000175:diagnosis* / Q000209:etiology; D005865:Gestational Age; D006801:Humans; D028421:Isoprostanes / Q000032:analysis* / Q000097:blood; D018384:Oxidative Stress; D011247:Pregnancy; D012307:Risk Factors
D015415:Biomarkers; D028421:Isoprostanes; C438786:isoprostane F2alpha III