Identification of novel allelic variants of integrin beta 2 (ITGB2) gene and screening for Bubaline leukocyte adhesion deficiency syndrome in Indian water buffaloes (Bubalus bubalis).
A fragment of 570 bp corresponding to exon 5 and 6 of integrin beta 2 (ITGB2) gene was amplified for screening D128G mutation in one hundred and fifty two buffaloes (Bubalus bubalis) which causes bovine leukocyte adhesion deficiency syndrome (BLAD) in cattle, as well as to ascertain polymorphism. TaqI PCR-RFLP revealed no such mutation thus indicating the absence of bubaline leukocyte adhesion deficiency (BuLAD) allele in animals under study. However, the polymorphism studies using MspI restriction enzyme revealed two genotypic patterns viz. AA pattern (bands of 293, 141, 105, and 31 bp) and BB pattern (bands of 293, 105, 77, 64, and 31 bp). The sequences of A and B alleles were submitted to the GenBank (EU853307 and AY821799).
Sharma
Deepak
D
Animal Genetics Division, Indian Veterinary Research Institute, Indian Council of Agricultural Research, Izatnagar, Bareilly (UP), India.
Kumar
Subodh
S
Deb
Sitangsu M
SM
Mitra
Abhijit
A
Niranjan
Saket K
SK
Naskar
Soumen
S
Sharma
Arjava
A
D000483:Alleles; D000818:Animals; D001483:Base Sequence; D002020:Buffaloes / Q000235:genetics* / Q000276:immunology*; D018821:CD18 Antigens / Q000235:genetics*; D002417:Cattle; D004252:DNA Mutational Analysis; D017931:DNA Primers / Q000235:genetics; D005820:Genetic Testing; D007194:India; D018370:Leukocyte-Adhesion Deficiency Syndrome / Q000235:genetics / Q000276:immunology / Q000662:veterinary*; D008969:Molecular Sequence Data; D012150:Polymorphism, Restriction Fragment Length; D013045:Species Specificity
D018821:CD18 Antigens; D017931:DNA Primers