[Growth characteristics and metabolomic sampling strategy of Lactobacillus sp. 30a(ATCC33222)].
OBJECTIVE
Sampling strategy for metabolomic studies of ATCC33222 was discussed according to its grown patterns under six different media.


METHODS
Six media with different ingredients or pH value were prepared and ATCC33222 strain cultures were harvested from each medium. Culture samples were collected every 1-4 hours and OD600 and pH values were determined thereafter. Growth curves and pH curves were constructed according to these data.


RESULTS
Growth curves were different between ATCC33222 strain cultures. MRS + aa pH 3.5 and MRS + aa + COS media showed longer lag phase, medium with additional glucose had the longest log phase, MRS + aa under pH 6.5 and 5.0 media showed shorter stationary phase. MRS, MRS + aa + glucose and MRS + aa + COS had similar pH curves while the other three pH curves were different from each other.


CONCLUSIONS
ATCC33222 strain may have the ability of dynamic metabolic'regulation to accommodate different culture environment or energy source. In metabolomic studies, pH 3.5 and pH 6.5 were recommend for culture condition. Amino acids could be used as ingredient in MRS while glucose was only optional, pH 5.4-5.9 could be important for lactic acid bacteria metabolomic studies.
Zhong
Kai
K
Institute of Nutrition and Food Safety, Chinese Center for Disease Control and Prevention, Beijing 100050, China.
He
Qinghua
Q
Wu
Yongning
Y
D003470:Culture Media; D006863:Hydrogen-Ion Concentration; D007778:Lactobacillus / Q000145:classification / Q000254:growth & development* / Q000378:metabolism*; D055432:Metabolomics / Q000379:methods*
D003470:Culture Media