title: A three-phase microfluidic chip for rapid sample clean-up of alkaloids from plant extracts.
abstract: A three-phase microchip was developed for the rapid and efficient small-scale purification of alkaloids from plant extracts. As part of the development of such a three-phase microchip, first a two-phase microchip with two channels (3.2 cm and 9.3 cm) was used to study the extraction efficiency of strychnine nitrate and strychnine at various flow rates. Strychnine was extracted from a basic aqueous phase to a chloroform phase (extraction) or strychnine was extracted from a chloroform phase into an acidic aqueous phase (back extraction). Subsequently, the "simultaneous extraction and back extraction" of strychnine was carried out in a three-phase microchip. The experimental extraction rate and yield were compared with model data. At a residence time of 25 sec, 79.5% of strychnine was extracted into the acidic aqueous phase using the three-phase microchip. In general, a good correlation was found between experimental results and model data for both two- and three-phase extractions. Finally, the three-phase microchip was employed in the purification of alkaloids (strychnine and brucine) from Strychnos seed extracts.
authors: Natural Products Chemistry Group, Laboratory of Organic Chemistry, Wageningen University, 6703 HB Wageningen, The Netherlands.
authors: Kishore K R
authors: KK
authors: Tetala
authors: Jan W
authors: JW
authors: Swarts
authors: Bo
authors: B
authors: Chen
authors: Anja E M
authors: AE
authors: Janssen
authors: Teris A
authors: TA
authors: van Beek
mesh_terms: D000470:Alkaloids / Q000737:chemistry / Q000302:isolation & purification*; D002725:Chloroform / Q000737:chemistry; D046210:Microfluidic Analytical Techniques / Q000379:methods*; D008956:Models, Chemical; D036103:Nanotechnology; D010936:Plant Extracts / Q000737:chemistry*; D012997:Solvents / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization; D013331:Strychnine / Q000031:analogs & derivatives / Q000737:chemistry / Q000302:isolation & purification; D013997:Time Factors
chemical_list: D000470:Alkaloids; D010936:Plant Extracts; D012997:Solvents; C083806:brucine; D002725:Chloroform; D013331:Strychnine