title: Grape-seed procyanidins modulate inflammation on human differentiated adipocytes in vitro.
abstract: Flavonoids are functional constituents of many fruits and vegetables. Procyanidins are flavonoids with an oligomeric structure, and it has been shown that they can improve the pathological oxidative state of a diabetic situation. To evaluate whether procyanidins can modulate inflammation, an event strongly associated with obesity, diabetes and insulin resistance states, we used human adipocytes (SGBS) and macrophage-like (THP-1) cell lines and administered an extract of grape-seed procyanidins (GSPE). THP-1 and SGBS cells pre-treated with GSPE showed a reduction of IL-6 and MCP-1 expression after an inflammatory stimulus. GSPE stimuli alone modulate adipokine (APM1 and LEP) and cytokine (IL-6 and MCP-1) gene expression. GSPE partially inhibited NF-kappaB translocation to the nucleus in both cell lines. These preliminary findings demonstrate that GSPE reduces the expression of IL-6 and MCP-1 and enhances the production of the anti-inflammatory adipokine adiponectin suggesting that may have a beneficial effect on low-grade inflammatory diseases such obesity and type 2 diabetes.
authors: Centro de Investigación Biomédica en Red de Diabetes y Enfermedades Metabólicas Asociadas (CIBERDEM), University Hospital of Tarragona, Joan XXIII, IISPV, Universitat Rovira i Virgili, Tarragona, Spain. mrodriguez.hj23.ics@gencat.cat
authors: Matilde R
authors: MR
authors: Chacón
authors: Victòria
authors: V
authors: Ceperuelo-Mallafré
authors: Elsa
authors: E
authors: Maymó-Masip
authors: Josep Maria
authors: JM
authors: Mateo-Sanz
authors: Lluis
authors: L
authors: Arola
authors: Cristina
authors: C
authors: Guitiérrez
authors: José Manuel
authors: JM
authors: Fernandez-Real
authors: Ana
authors: A
authors: Ardèvol
authors: Imma
authors: I
authors: Simón
authors: Joan
authors: J
authors: Vendrell
mesh_terms: D017667:Adipocytes / Q000166:cytology / Q000187:drug effects / Q000276:immunology*; D054392:Adipokines / Q000378:metabolism; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D002454:Cell Differentiation; D002460:Cell Line; D016207:Cytokines / Q000378:metabolism*; D006801:Humans; D018836:Inflammation Mediators / Q000378:metabolism; D008264:Macrophages / Q000187:drug effects / Q000276:immunology; D016328:NF-kappa B / Q000378:metabolism; D010936:Plant Extracts / Q000494:pharmacology; D044945:Proanthocyanidins / Q000494:pharmacology*; D012639:Seeds / Q000737:chemistry; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology; D027843:Vitis / Q000737:chemistry*
chemical_list: D054392:Adipokines; D000893:Anti-Inflammatory Agents; D016207:Cytokines; D018836:Inflammation Mediators; D016328:NF-kappa B; D010936:Plant Extracts; D044945:Proanthocyanidins; D014409:Tumor Necrosis Factor-alpha