Salivary levels of IL-1beta, IL-6, IL-8, and TNF-alpha in patients with burning mouth syndrome.
OBJECTIVE
To compare salivary IL-1beta, IL-6, IL-8, and TNF-alpha levels between patients with burning mouth syndrome (BMS) and controls.


METHODS
Forty female patients with BMS (mean age: 61.6+/-10.1 years) and 20 female control subjects (mean age: 65.1+/-9.0 years) were included in the study. Unstimulated (UWS) and stimulated whole saliva samples (SWS) were collected and their flow rates were determined. Salivary IL-1beta, IL-6, IL-8, and TNF-alpha levels and total protein concentration were also determined. Salivary transferrin level was determined to investigate the level of blood contamination in saliva samples. Gingival index of the subjects was also examined. Student's t-test, Pearson's correlation analysis, and analysis of covariance were used.


RESULTS
No significant differences were found in the salivary levels of IL-1beta, IL-6, IL-8, and TNF-alpha in BMS patients compared with controls. Salivary flow rates and their total protein concentrations did not differ significantly between the groups. The levels of salivary cytokines and total protein concentration correlated significantly with the level of blood contamination in both UWS and SWS.


CONCLUSIONS
There were no differences in the salivary levels of IL-1beta, IL-6, IL-8, and TNF-alpha in BMS patients compared with controls. Cytokine levels in whole saliva were affected mainly by the amount of blood contamination.
Suh
Kyung-Im
KI
Dept. of Oral Medicine and Oral Diagnosis, School of Dentistry and Dental Research Institute, Seoul National University, Yunkeun-Dong 28, Chongro-Ku, Seoul 110-749, Republic of Korea.
Kim
Young-Ku
YK
Kho
Hong-Seop
HS
D000368:Aged; D001769:Blood; D002054:Burning Mouth Syndrome / Q000378:metabolism*; D016022:Case-Control Studies; D005260:Female; D005884:Gingival Hemorrhage / Q000145:classification; D005891:Gingivitis / Q000145:classification; D006801:Humans; D053583:Interleukin-1beta / Q000032:analysis*; D015850:Interleukin-6 / Q000032:analysis*; D016209:Interleukin-8 / Q000032:analysis*; D008875:Middle Aged; D010512:Periodontal Index; D012463:Saliva / Q000737:chemistry* / Q000378:metabolism; D012471:Salivary Proteins and Peptides / Q000032:analysis; D012636:Secretory Rate; D014168:Transferrin / Q000032:analysis; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis*
D053583:Interleukin-1beta; D015850:Interleukin-6; D016209:Interleukin-8; D012471:Salivary Proteins and Peptides; D014168:Transferrin; D014409:Tumor Necrosis Factor-alpha