Multiphoton adaptation of a commercial low-cost confocal microscope for live tissue imaging.
The Nikon C1 confocal laser scanning microscope is a relatively inexpensive and user-friendly instrument. We describe a straightforward method to convert the C1 for multiphoton microscopy utilizing direct coupling of a femtosecond near-infrared laser into the scan head and fiber optic transmission of emission light to the three-channel detector box. Our adapted system can be rapidly switched between confocal and multiphoton mode, requires no modification to the original system, and uses only a few custom-made parts. The entire system, including scan mirrors and detector box, remain under the control of the user-friendly Nikon EZ-C1 software without modification.
Mancuso
James J
JJ
Baylor College of Medicine, Verna and Marrs McLean Department of Biochemistry, One Baylor Plaza, Houston, Texas 77030, USA.
Larson
Adam M
AM
Wensel
Theodore G
TG
Saggau
Peter
P
D000818:Animals; D004867:Equipment Design; D049452:Green Fluorescent Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics; D006801:Humans; D007091:Image Processing, Computer-Assisted / Q000295:instrumentation / Q000379:methods; D051379:Mice; D008822:Mice, Transgenic; D018613:Microscopy, Confocal / Q000295:instrumentation* / Q000379:methods; D036641:Microscopy, Fluorescence, Multiphoton / Q000295:instrumentation* / Q000379:methods; D009474:Neurons; D055100:Optical Fibers; D011058:Pollen / Q000648:ultrastructure; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics; D012160:Retina / Q000166:cytology / Q000378:metabolism; D012243:Rhodopsin / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics
D011993:Recombinant Fusion Proteins; D049452:Green Fluorescent Proteins; D012243:Rhodopsin