Localized electrochemistry on a 10 microm spot on a monolith large electrode: an avenue for electrochemical microarray analysis.
By measurement of the modulation of ions under an ac field at the electrode/electrolyte interface layer (EIL) at a spatial modulation of 0.0001 nm, a quantitative measurement of local redox current on a 10 microm spot of a working electrode is achieved at a sensitivity of 0.02 nA/spot. The spot is defined by the probe laser beam. We show the principle for two redox ions, [Fe(CN)(6)](3-) and [Ru(NH(3))(6)](3+), on a 4 mm(2) Au electrode. The redox peaks from the optical and the electrochemical (cyclic voltammetry (CV)) responses, which are concomitantly measured, are within 0.005 V. The setup is identical to the conventional three-electrode measurement, and the laser beam does not perturb the electrochemical process. We demonstrate high sensitivity to "read" binding between two single-stranded DNA (ssDNA) on a 10 microm spot for DNA microarray analysis where the signal is "blind" to nonspecific binding. Potentially, quantitative combinatorial electrochemistry can be performed on a monolith electrode by scanning the laser beam on the electrode.
Singh
Gaurav
G
Chemical and Biomolecular Engineering, University of Nebraska-Lincoln, Lincoln, Nebraska 68588-0642, USA.
Moore
David
D
Saraf
Ravi F
RF
D015374:Biosensing Techniques; D004277:DNA, Single-Stranded / Q000737:chemistry*; D004563:Electrochemistry; D004566:Electrodes; D053768:Metal Nanoparticles / Q000737:chemistry; D036103:Nanotechnology; D020411:Oligonucleotide Array Sequence Analysis; D010084:Oxidation-Reduction
D004277:DNA, Single-Stranded