Respiratory syncytial virus subunit vaccine based on a recombinant fusion protein expressed transiently in mammalian cells.
Although respiratory syncytial virus (RSV) causes severe lower respiratory tract infection in infants and adults at risk, no RSV vaccine is currently available. In this report, efforts toward the generation of an RSV subunit vaccine using recombinant RSV fusion protein (rRSV-F) are described. The recombinant protein was produced by transient gene expression (TGE) in suspension-adapted human embryonic kidney cells (HEK-293E) in 4 L orbitally shaken bioreactors. It was then purified and formulated in immunostimulating reconstituted influenza virosomes (IRIVs). The candidate vaccine induced anti-RSV-F neutralizing antibodies in mice, and challenge studies in cotton rats are ongoing. If successful in preclinical and clinical trials, this will be the first recombinant subunit vaccine produced by large-scale TGE in mammalian cells.
Nallet
Sophie
S
Ecole Polytechnique Fédérale de Lausanne (EPFL), Institute of Bioengineering, Laboratory of Cellular Biotechnology, CH-1015 Lausanne, Switzerland.
Amacker
Mario
M
Westerfeld
Nicole
N
Baldi
Lucia
L
König
Iwo
I
Hacker
David L
DL
Zaborosch
Christiane
C
Zurbriggen
Rinaldo
R
Wurm
Florian M
FM
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D002460:Cell Line; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D009500:Neutralization Tests; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification; D018357:Respiratory Syncytial Virus Infections / Q000276:immunology / Q000517:prevention & control*; D022261:Respiratory Syncytial Virus Vaccines / Q000276:immunology*; D014162:Transfection; D022223:Vaccines, Subunit / Q000276:immunology; D014760:Viral Fusion Proteins / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification; D022701:Virosomes / Q000276:immunology
D000914:Antibodies, Viral; C526850:F protein, human respiratory syncytial virus; D011993:Recombinant Fusion Proteins; D022261:Respiratory Syncytial Virus Vaccines; D022223:Vaccines, Subunit; D014760:Viral Fusion Proteins; D022701:Virosomes