title: Vitamin B12 is the active corrinoid produced in cultivated white button mushrooms (Agaricus bisporus).
abstract: Analysis of vitamin B(12) in freshly harvested white button mushrooms ( Agaricus bisporus ) from five farms was performed by affinity chromatography and HPLC-ESI-MS techniques. The vitamin B(12) concentrations obtained varied from farm to farm, with higher concentrations of vitamin B(12) detected in outer peel than in cap, stalk, or flesh, suggesting that the vitamin B(12) is probably bacteria-derived. High concentrations of vitamin B(12) were also detected in the flush mushrooms including cups and flats. HPLC and mass spectrometry showed vitamin B(12) retention time and mass spectra identical to those of the standard vitamin B(12) and those of food products including beef, beef liver, salmon, egg, and milk but not of the pseudovitamin B(12), an inactive corrinoid in humans. The results suggest that the consumer may benefit from the consumption of mushroom to increase intake of this vitamin in the diet.
authors: Centre for Plant and Food Science, College of Health and Science, University of Western Sydney, Locked Bag 1797, Penrith South DC, NSW 1797, Australia.
authors: Sundar Rao
authors: SR
authors: Koyyalamudi
authors: Sang-Chul
authors: SC
authors: Jeong
authors: Kai Yip
authors: KY
authors: Cho
authors: Gerald
authors: G
authors: Pang
mesh_terms: D000364:Agaricus / Q000737:chemistry / Q000378:metabolism*; D000383:Agriculture; D002851:Chromatography, High Pressure Liquid; D005504:Food Analysis; D021241:Spectrometry, Mass, Electrospray Ionization; D014805:Vitamin B 12 / Q000032:analysis / Q000096:biosynthesis*
chemical_list: D014805:Vitamin B 12