title: Standardization of flow cytometry in myelodysplastic syndromes: report from the first European LeukemiaNet working conference on flow cytometry in myelodysplastic syndromes.
abstract: The myelodysplastic syndromes are a group of clonal hematopoietic stem cell diseases characterized by cytopenia(s), dysplasia in one or more cell lineages and increased risk of evolution to acute myeloid leukemia (AML). Recent advances in immunophenotyping of hematopoietic progenitor and maturing cells in dysplastic bone marrow point to a useful role for multiparameter flow cytometry (FCM) in the diagnosis and prognostication of myelodysplastic syndromes. In March 2008, representatives from 18 European institutes participated in a European LeukemiaNet (ELN) workshop held in Amsterdam as a first step towards standardization of FCM in myelodysplastic syndromes. Consensus was reached regarding standard methods for cell sampling, handling and processing. The group also defined minimal combinations of antibodies to analyze aberrant immunophenotypes and thus dysplasia. Examples are altered numbers of CD34(+) precursors, aberrant expression of markers on myeloblasts, maturing myeloid cells, monocytes or erythroid precursors and the expression of lineage infidelity markers. When applied in practice, aberrant FCM patterns correlate well with morphology, the subclassification of myelodysplastic syndromes, and prognostic scoring systems. However, the group also concluded that despite strong evidence for an impact of FCM in myelodysplastic syndromes, further (prospective) validation of markers and immunophenotypic patterns are required against control patient groups as well as further standardization in multi-center studies. Standardization of FCM in myelodysplastic syndromes may thus contribute to improved diagnosis and prognostication of myelodysplastic syndromes in the future.
authors: Department of Hematology, VU University Medical Center, Amsterdam, The Netherlands. a.vandeloosdrecht@vumc.nl
authors: Arjan A
authors: AA
authors: van de Loosdrecht
authors: Canan
authors: C
authors: Alhan
authors: Marie Christine
authors: MC
authors: Béné
authors: Matteo G
authors: MG
authors: Della Porta
authors: Angelika M
authors: AM
authors: Dräger
authors: Jean
authors: J
authors: Feuillard
authors: Patricia
authors: P
authors: Font
authors: Ulrich
authors: U
authors: Germing
authors: Detlef
authors: D
authors: Haase
authors: Christa H
authors: CH
authors: Homburg
authors: Robin
authors: R
authors: Ireland
authors: Joop H
authors: JH
authors: Jansen
authors: Wolfgang
authors: W
authors: Kern
authors: Luca
authors: L
authors: Malcovati
authors: Jeroen G
authors: JG
authors: Te Marvelde
authors: Ghulam J
authors: GJ
authors: Mufti
authors: Kiyoyuki
authors: K
authors: Ogata
authors: Alberto
authors: A
authors: Orfao
authors: Gert J
authors: GJ
authors: Ossenkoppele
authors: Anna
authors: A
authors: Porwit
authors: Frank W
authors: FW
authors: Preijers
authors: Stephen J
authors: SJ
authors: Richards
authors: Gerrit Jan
authors: GJ
authors: Schuurhuis
authors: Dolores
authors: D
authors: Subirá
authors: Peter
authors: P
authors: Valent
authors: Vincent H J
authors: VH
authors: van der Velden
authors: Paresh
authors: P
authors: Vyas
authors: August H
authors: AH
authors: Westra
authors: Theo M
authors: TM
authors: de Witte
authors: Denise A
authors: DA
authors: Wells
authors: Michael R
authors: MR
authors: Loken
authors: Theresia M
authors: TM
authors: Westers
mesh_terms: D015703:Antigens, CD / Q000276:immunology; D005434:Flow Cytometry / Q000379:methods* / Q000592:standards; D006801:Humans; D016130:Immunophenotyping / Q000379:methods; D009190:Myelodysplastic Syndromes / Q000175:diagnosis* / Q000276:immunology; D012015:Reference Standards
chemical_list: D015703:Antigens, CD