Self-assembled dithiothreitol on Au surfaces for biological applications: phospholipid bilayer formation.
Self-assembly of dithiothreitol (DTT) on Au(111) from solution deposition has been studied by X-ray photoelectron spectroscopy and electrochemical data. DTT molecules self-assemble on Au(111) in a lying-down configuration irrespective of the concentration and temperature. XPS and electrochemical data indicate a DTT surface coverage of theta approximately 0.16 with two S-head-Au covalent bonds per DTT molecule. The DTT monolayer turns the Au surface hydrophilic enough to allow the formation of fluid dimyristoylphosphatidylcholine (DMPC) bilayer domains by vesicle fusion as revealed by in situ atomic force imaging. Methylene blue (MB) and flavin adenine dinucleotide (FAD) have been used as probes to study molecule transport across the bilayer.
Creczynski-Pasa
Tânia B
TB
Departamento de Ciências Farmacêuticas and Departamento de Física, UFSC, C. P. 476, Florianópolis, 88.040-900, Brazil. pasa@fisica.ufsc.br
Millone
M Antonieta Daza
MA
Munford
Maximiliano L
ML
de Lima
Vânia R
VR
Vieira
Tiago O
TO
Benitez
Guillermo A
GA
Pasa
André A
AA
Salvarezza
Roberto C
RC
Vela
María E
ME
D002462:Cell Membrane / Q000737:chemistry / Q000187:drug effects; D004134:Dimyristoylphosphatidylcholine / Q000737:chemistry; D004229:Dithiothreitol / Q000737:chemistry* / Q000494:pharmacology; D004563:Electrochemistry; D005182:Flavin-Adenine Dinucleotide / Q000737:chemistry; D006046:Gold / Q000737:chemistry*; D008051:Lipid Bilayers / Q000737:chemistry*; D008751:Methylene Blue / Q000737:chemistry; D015335:Molecular Probes / Q000737:chemistry; D010743:Phospholipids / Q000737:chemistry*; D013057:Spectrum Analysis; D013455:Sulfur / Q000737:chemistry; D013499:Surface Properties
D008051:Lipid Bilayers; D015335:Molecular Probes; D010743:Phospholipids; D005182:Flavin-Adenine Dinucleotide; D013455:Sulfur; D006046:Gold; D008751:Methylene Blue; D004229:Dithiothreitol; D004134:Dimyristoylphosphatidylcholine