Electrochemical behavior of lactate dehydrogenase immobilized on "silica sol-gel/nanometre-sized tridecameric aluminum polycation" modified gold electrode and its application.
This paper reports the electrochemical behavior of lactate dehydrogenase (LDH) immobilized in the silica sol-gel film on gold electrode after adding nanometre-sized tridecameric aluminium polycation (nano-Al13, also called nanopolynuclear Al13) as a promoter. A pair of surface controlled quasi-reversible cyclic voltammetry peaks with the formal potential (E0') of 154 mV (vs. SCE) was found in the presence of nano-Al13. A potential application of the nano-Al13-LDH electrode for the determination of resorcinol and p-xylene was also investigated. The experimental results showed that both resorcinol and p-xylene inhibited LDH activity, and the calibration ranges were 5.0 x 10(-6)-3.0 x 10(-4) mol L(-1) for resorcinol and 1.0 x 10(-6)-1.0 x 10(-5) mol L(-1) for p-xylene, respectively. The nano-Al13-LDH electrode can be anticipated to be applied to environmental toxic assessments.
Cheng
Jiongjia
J
School of Chemistry & Chemical Engineering, State Key Laboratory of Coordination Chemistry of China & Key Laboratory of MOE for Life Science, Nanjing University, Nanjing, 210093, People's Republic of China.
Huang
Deqian
D
Zhang
Jing
J
Yang
Wenjing
W
Wang
Na
N
Sun
Yongbo
Y
Wang
Keyu
K
Mo
Xiangyin
X
Bi
Shuping
S
D000535:Aluminum / Q000737:chemistry*; D000818:Animals; D015374:Biosensing Techniques; D002417:Cattle; D004563:Electrochemistry; D004566:Electrodes; D004785:Environmental Pollutants / Q000032:analysis; D004800:Enzymes, Immobilized / Q000737:chemistry* / Q000378:metabolism; D005782:Gels; D006046:Gold / Q000737:chemistry*; D007770:L-Lactate Dehydrogenase / Q000737:chemistry* / Q000378:metabolism; D053768:Metal Nanoparticles / Q000737:chemistry*; D012822:Silicon Dioxide / Q000737:chemistry*
D004785:Environmental Pollutants; D004800:Enzymes, Immobilized; D005782:Gels; D006046:Gold; D012822:Silicon Dioxide; D000535:Aluminum; D007770:L-Lactate Dehydrogenase