Solvation effects in the quartz crystal microbalance with dissipation monitoring response to biomolecular adsorption. A phenomenological approach.
Quartz crystal microbalance with dissipation monitoring (QCM-D) has become a popular tool to investigate biomolecular adsorption phenomena at surfaces. In contrast to optical mass-sensitive techniques, which commonly detect the adsorbed nonhydrated mass, the mechanically coupled mass measured by QCM-D includes a significant amount of water. A mechanistic and quantitative picture of how the surrounding liquid couples to the deposited solutes has so far been elusive for apparently simple phenomena like the random adsorption of nanometer-sized particles on a planar surface. Using a setup that enables simultaneous measurements by reflectometry and QCM-D on the same support, we have quantified the variations in coupled water, as sensed by the QCM frequency response, as a function of coverage for the formation of monolayers of globular proteins, virus particles, and small unilamellar vesicles. We found a close-to-linear relationship between the surface coverage and the relative contribution of water to the frequency response for these adsorption scenarios. The experimental hydration curves could be reproduced quantitatively using a theoretical model that assigns a pyramid-shaped hydration coat to each adsorbed particle and that accounts for the random distribution of adsorbents on the surface. This simple model fits the experimental data well and provides insight into the parameters that affect hydration.
Bingen
Pit
P
Biosurfaces Unit, CIC biomaGUNE, Paseo Miramon 182, 20009 Donostia, San Sebastian, Spain.
Wang
Guoliang
G
Steinmetz
Nicole F
NF
Rodahl
Michael
M
Richter
Ralf P
RP
D000327:Adsorption; D001360:Avidin / Q000737:chemistry; D017798:Comovirus / Q000737:chemistry*; D008832:Microchemistry / Q000295:instrumentation / Q000379:methods*; D008956:Models, Chemical; D010316:Particle Size; D011506:Proteins / Q000737:chemistry*; D011791:Quartz; D019809:Streptavidin / Q000737:chemistry; D013499:Surface Properties; D053835:Unilamellar Liposomes / Q000737:chemistry*; D014867:Water / Q000737:chemistry*
D011506:Proteins; D053835:Unilamellar Liposomes; D014867:Water; D001360:Avidin; D011791:Quartz; D019809:Streptavidin