Local anesthetics induce chondrocyte death in bovine articular cartilage disks in a dose- and duration-dependent manner.
OBJECTIVE
The purpose of this study was to evaluate the effect of various local anesthetics on chondrocyte viability in articular cartilage by use of a bovine disk model.


METHODS
Full-thickness bovine cartilage disks were isolated from the condylar surfaces of the radial-carpal joint by use of a 4-mm biopsy punch and were incubated in various concentrations of local anesthetics (e.g., bupivacaine) for varying amounts of time and stained for membrane integrity by use of ethidium bromide and SYTO 13 stain (Molecular Probes, Carlsbad, CA). Cell and nuclear morphology was assessed by transmission electron microscopy.


RESULTS
The addition of local anesthetics (i.e., 0.25% bupivacaine, 1% lidocaine, and 0.5% ropivacaine) to bovine articular cartilage disks had a negative effect on chondrocyte viability. Culturing bovine articular cartilage disks for increasing periods of time decreased chondrocyte viability for each of the local anesthetics, with significant negative correlations being shown between time of exposure to the drug and chondrocyte viability. These effects were also affected by the presence or absence of epinephrine in local anesthetic preparations.


CONCLUSIONS
Our results suggest that local anesthetics (i.e., bupivacaine, lidocaine, or ropivacaine) can have a detrimental effect on chondrocyte viability in bovine articular cartilage disks in a dose- and duration-dependent manner.


CONCLUSIONS
After arthroscopic surgery, it has been common practice to inject various local anesthetics into the joint for pain relief. Because adult chondrocytes have little or no capacity to regenerate, these results suggest that high-dose, long-term intra-articular administration of local anesthetics should be performed with caution.
Lo
Ian K Y
IK
Department of Surgery, Faculty of Medicine, University of Calgary, Calgary, Alberta, Canada. ikylo@ucalgary.ca
Sciore
Paul
P
Chung
May
M
Liang
Sherri
S
Boorman
Richard B
RB
Thornton
Gail M
GM
Rattner
Jerome B
JB
Muldrew
Kenneth
K
D000577:Amides / Q000494:pharmacology; D000779:Anesthetics, Local / Q000633:toxicity*; D000818:Animals; D002045:Bupivacaine / Q000494:pharmacology; D002358:Cartilage, Articular / Q000187:drug effects* / Q000473:pathology; D002417:Cattle; D018929:Cell Culture Techniques; D016923:Cell Death; D002470:Cell Survival / Q000187:drug effects; D019902:Chondrocytes / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D008012:Lidocaine / Q000494:pharmacology; D023421:Models, Animal; D000077212:Ropivacaine; D013997:Time Factors
D000577:Amides; D000779:Anesthetics, Local; D000077212:Ropivacaine; D008012:Lidocaine; D002045:Bupivacaine