title: High heterogeneity of plasma membrane microfluidity in multidrug-resistant cancer cells.
abstract: Diffusion-time distribution analysis (DDA) has been used to explore the plasma membrane fluidity of multidrug-resistant cancer cells (LR73 carcinoma cells) and also to characterize the influence of various membrane agents present in the extracellular medium. DDA is a recent single-molecule technique, based on fluorescence correlation spectroscopy (FCS), well suited to retrieve local organization of cell membrane. The method was conducted on a large number of living cells, which enabled us to get a detailed overview of plasma membrane microviscosity, and plasma membrane micro-organization, between the cells of the same line. Thus, we clearly reveal the higher heterogeneity of plasma membrane in multidrug-resistant cancer cells in comparison with the nonresistant ones (denoted sensitive cells). We also display distinct modifications related to a membrane fluidity modulator, benzyl alcohol, and two revertants of multidrug resistance, verapamil and cyclosporin-A. A relation between the distribution of the diffusion-time values and the modification of membrane lateral heterogeneities is proposed.
authors: Universite de Technologie de Troyes, Laboratoire de Nanotechnologie et d'Instrumentation Optique, LRC CEA, Institut Charles Delaunay, FRE CNRS 2848, 12 rue Marie Curie, BP 2060, Troyes Cedex 10010, France.
authors: Céline
authors: C
authors: Boutin
authors: Yann
authors: Y
authors: Roche
authors: Christine
authors: C
authors: Millot
authors: Régis
authors: R
authors: Deturche
authors: Pascal
authors: P
authors: Royer
authors: Michel
authors: M
authors: Manfait
authors: Jéro Me
authors: JM
authors: Plain
authors: Pierre
authors: P
authors: Jeannesson
authors: Jean-Marc
authors: JM
authors: Millot
authors: Rodolphe
authors: R
authors: Jaffiol
mesh_terms: D000465:Algorithms; D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D019905:Benzyl Alcohol / Q000494:pharmacology; D016466:CHO Cells; D045744:Cell Line, Tumor; D002462:Cell Membrane / Q000737:chemistry* / Q000187:drug effects; D006224:Cricetinae; D003412:Cricetulus; D016572:Cyclosporine / Q000494:pharmacology; D004058:Diffusion; D018432:Drug Resistance, Multiple; D019008:Drug Resistance, Neoplasm; D004867:Equipment Design; D005260:Female; D008563:Membrane Lipids / Q000737:chemistry; D051379:Mice; D044085:Microfluidics / Q000379:methods*; D008954:Models, Biological; D010051:Ovarian Neoplasms / Q000737:chemistry* / Q000188:drug therapy*; D013050:Spectrometry, Fluorescence / Q000379:methods*; D018709:Statistics, Nonparametric; D013997:Time Factors; D014700:Verapamil / Q000494:pharmacology; D014783:Viscosity
chemical_list: D000970:Antineoplastic Agents; D008563:Membrane Lipids; D016572:Cyclosporine; D014700:Verapamil; D019905:Benzyl Alcohol