Noninvasive characterization of the fission yeast cell cycle by monitoring dry mass with digital holographic microscopy.
Digital holography microscopy (DHM) is an optical technique which provides phase images yielding quantitative information about cell structure and cellular dynamics. Furthermore, the quantitative phase images allow the derivation of other parameters, including dry mass production, density, and spatial distribution. We have applied DHM to study the dry mass production rate and the dry mass surface density in wild-type and mutant fission yeast cells. Our study demonstrates the applicability of DHM as a tool for label-free quantitative analysis of the cell cycle and opens the possibility for its use in high-throughput screening.
Rappaz
Benjamin
B
Ecole Polytechnique Federale de Lausanne, Brain Mind Institute, CH-1015 Lausanne, Switzerland. benjamin.rappaz@mcgill.ca
Cano
Elena
E
Colomb
Tristan
T
Kühn
Jonas
J
Depeursinge
Christian
C
Simanis
Viesturs
V
Magistretti
Pierre J
PJ
Marquet
Pierre
P
D018797:Cell Cycle Proteins / Q000235:genetics; D048749:Cytokinesis / Q000502:physiology; D006696:Holography / Q000379:methods*; D016014:Linear Models; D008853:Microscopy / Q000379:methods*; D009154:Mutation; D012568:Schizosaccharomyces / Q000166:cytology* / Q000254:growth & development; D029702:Schizosaccharomyces pombe Proteins / Q000235:genetics
D018797:Cell Cycle Proteins; D029702:Schizosaccharomyces pombe Proteins; C498281:cdc16 protein, S pombe