title: Experimental orthotopic lung transplantation model in rats with cold storage.
abstract: This report describes a new experimental procedure, a rat unilateral, orthotopic lung transplantation with cold storage, and evaluates its relevancy and reliability to study the early events during cold ischemia/reperfusion (I/R) injury. This model, using the cuff technique, does not require extensive training and is relatively easy to be established. The model can induce reproducible degrees of pulmonary graft injury including impaired gas exchange, proinflammatory cytokine upregulation, or inflammatory infiltrates, depending on the preservation time. The results are consistent with the previous clinical evidence, thus suggesting that this model is a valid and reliable animal model of cold I/R injury.
authors: Heart, Lung and Esophageal Surgery Institute, Department of Surgery, University of Pittsburgh, Pittsburgh, PA, USA.
authors: Ryujiro
authors: R
authors: Sugimoto
authors: Atsunori
authors: A
authors: Nakao
authors: Itaru
authors: I
authors: Nagahiro
authors: Junichi
authors: J
authors: Kohmoto
authors: Seiichiro
authors: S
authors: Sugimoto
authors: Mikio
authors: M
authors: Okazaki
authors: Masaomi
authors: M
authors: Yamane
authors: Hidetoshi
authors: H
authors: Inokawa
authors: Takahiro
authors: T
authors: Oto
authors: Kazunori
authors: K
authors: Tahara
authors: Jianghua
authors: J
authors: Zhan
authors: Yoshifumi
authors: Y
authors: Sano
authors: Kenneth R
authors: KR
authors: McCurry
mesh_terms: D000818:Animals; D015925:Cryopreservation; D004195:Disease Models, Animal; D055370:Lung Injury / Q000209:etiology*; D016040:Lung Transplantation / Q000009:adverse effects* / Q000379:methods; D008297:Male; D009926:Organ Preservation; D051381:Rats; D011917:Rats, Inbred Lew; D015427:Reperfusion Injury / Q000209:etiology*