Anion sensor-based ratiometric peptide probe for protein kinase activity.
A new fluorescent sensor consisting of Cd(II)-cylcen appended aminocoumarin and a substrate peptide for protein kinase A (PKA) has been designed. Upon phosphorylation by PKA, the metal complex moiety binds to a phosphorylated residue, which in turn displaces the coumarin fluorophore, and this event results in ratiometric change of excitation spectrum in neutral aqueous solution.
Kikuchi
Kazuya
K
PRESTO, JST Corporation, Kawaguchi, Saitama, Japan.
Hashimoto
Shigeki
S
Mizukami
Shin
S
Nagano
Tetsuo
T
D000838:Anions; D015374:Biosensing Techniques / Q000379:methods; D002104:Cadmium / Q000737:chemistry; D003374:Coumarins / Q000138:chemical synthesis* / Q000737:chemistry; D017868:Cyclic AMP-Dependent Protein Kinases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D005456:Fluorescent Dyes / Q000138:chemical synthesis* / Q000737:chemistry; D015394:Molecular Structure; D010455:Peptides / Q000032:analysis / Q000494:pharmacology; D010766:Phosphorylation; D014867:Water / Q000737:chemistry
D000838:Anions; D003374:Coumarins; D005456:Fluorescent Dyes; D010455:Peptides; D002104:Cadmium; D014867:Water; D017868:Cyclic AMP-Dependent Protein Kinases