Differential transcription-activating capability of NS1 proteins from different influenza virus subtypes expressed in yeast.
Influenza A virus NS1 protein is an important regulatory factor with multiple functions and contributes greatly to viral pathogenesis. In the present study, transcription-activating potential of NS1 from different influenza A virus subtypes was examined in yeast two-hybrid system. The bait vectors containing different NS1 genes, along with an empty prey vector, were transformed into yeast AH109(for growth assay on QDO plate and alpha-galactosidase assay), and Y187(for beta-galactosidase assay). AH109 transformants with NS1 gene from H1N1, H5N1, and H9N2 viruses grew vigorously on the QDO plate and secreted high level of alpha-galactosidase. Also, Y187 bearing the above NS1 genes exhibited enhanced beta-galactosidase activity. Nevertheless, H3N2-NS1-transformed AH109 and Y187 yeasts did not grow on QDO plate and secrete beta-galactosidase, respectively. These findings denote the remarkable variation in NS1 proteins from different influenza A virus subtypes on the transcription-stimulating capability in yeast.
LI
WeiZhong
W
Department of Microbiology and Immunology, Shantou University Medical College, Shantou 515041, China.
WANG
GeFei
G
ZENG
Jun
J
ZHANG
DanGui
D
ZHANG
Heng
H
CHEN
XiaoXuan
X
CHEN
YouYing
Y
LI
KangSheng
K
D000818:Animals; D006801:Humans; D009980:Influenza A virus / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D015533:Transcriptional Activation; D020798:Two-Hybrid System Techniques; D017361:Viral Nonstructural Proteins / Q000235:genetics / Q000378:metabolism*; D015003:Yeasts / Q000235:genetics / Q000378:metabolism; D000519:alpha-Galactosidase / Q000378:metabolism; D001616:beta-Galactosidase / Q000378:metabolism
C085820:INS1 protein, influenza virus; D017361:Viral Nonstructural Proteins; D000519:alpha-Galactosidase; D001616:beta-Galactosidase