GDI-1 preferably interacts with Rab10 in insulin-stimulated GLUT4 translocation.
Insulin stimulates GLUT4 (glucose transporter 4) translocation in adipocytes and muscles. An emerging picture is that Rab10 could bridge the gap between the insulin signalling cascade and GLUT4 translocation in adipocytes. In the present study, two potential effectors of Rab10, GDI (guanine-nucleotide-dissociation inhibitor)-1 and GDI-2, are characterized in respect to their roles in insulin-stimulated GLUT4 translocation. It is shown that both GDI-1 and GDI-2 exhibit similar distribution to GLUT4 and Rab10 at the TGN (trans-Golgi network) and periphery structures. Meanwhile, GDI-1 clearly interacts with Rab10 with higher affinity, as shown by both immunoprecipitation and in vivo FRET (fluorescence resonance energy transfer). In addition, the participation of GDIs in GLUT4 translocation is illustrated when overexpression of either GDI inhibits insulin-stimulated GLUT4 translocation in 3T3-L1 adipocytes. Taken together, we propose that GDI-1 is preferentially involved in insulin-stimulated GLUT4 translocation through facilitating Rab10 recycling.
Chen
Yu
Y
National Key Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing, 100101, China.
Deng
Yongqiang
Y
Zhang
Jinzhong
J
Yang
Lu
L
Xie
Xiangyang
X
Xu
Tao
T
D017667:Adipocytes / Q000187:drug effects / Q000378:metabolism; D000818:Animals; D002460:Cell Line; D051275:Glucose Transporter Type 4 / Q000378:metabolism*; D020730:Guanine Nucleotide Dissociation Inhibitors / Q000378:metabolism* / Q000502:physiology; D006801:Humans; D007328:Insulin / Q000494:pharmacology*; D051379:Mice; D041681:NIH 3T3 Cells; D011485:Protein Binding / Q000187:drug effects / Q000502:physiology; D021381:Protein Transport; D020691:rab GTP-Binding Proteins / Q000378:metabolism*
C086796:GDP dissociation inhibitor 1; D051275:Glucose Transporter Type 4; D020730:Guanine Nucleotide Dissociation Inhibitors; D007328:Insulin; C538901:Rab10 protein, human; D020691:rab GTP-Binding Proteins