Accurate molecular weight analysis of histones using FFE and RP-HPLC on monolithic capillary columns.
Due to their large diversity with respect to post-translational modifications (PTMs), the family of histones provides a major analytical challenge in current proteomics research. Their function has a large impact on the transcription of DNA, and as a result, on the expression of proteins. The variation in PTMs regulates transcription, and, as a result, many methods are being employed for the in-depth analysis of histones. In this paper, we present a separation strategy for histones based on free-flow electrophoresis (FFE) followed by an RP separation on capillary monolithic PS-DVB columns. The capillary columns are directly interfaced with an FT-ICR MS providing an online system for the detection and accurate molecular weight analysis of intact histones.
Sneekes
Evert-Jan
EJ
Dionex Corporation, Amsterdam, The Netherlands.
Han
Jun
J
Elliot
Monica
M
Ausio
Juan
J
Swart
Remco
R
Heck
Albert J R
AJ
Borchers
Christoph
C
D000818:Animals; D002417:Cattle; D002645:Chickens; D002853:Chromatography, Liquid / Q000295:instrumentation / Q000379:methods; D019075:Electrophoresis, Capillary / Q000295:instrumentation / Q000379:methods; D006657:Histones / Q000032:analysis / Q000302:isolation & purification; D013058:Mass Spectrometry / Q000295:instrumentation / Q000379:methods; D008970:Molecular Weight
D006657:Histones