title: Connexin43 interacts with Caveolin-3 in the heart.
abstract: Gap junctions (GJs), collections of multiple intercellular channels between neighboring cells, are specialized channels facilitating intercellular electrical and chemical communication. GJs are important for synchronizing coupling and coordinated contraction in the heart, and are crucial regulators of heart gene transcription, cardiac development, and protection of ischemic cardiomyocytes through second messenger communication. Identification of proteins that interact with Connexin43 (Cx43), the predominant protein in cardiac GJs, may contribute to the understanding of GJ functional regulation. Using a yeast two-hybrid system, we identified Caveolin-3 (Cav3) as a new Cx43-interacting protein. This interaction was confirmed by co-immunoprecipitation and co-localization experiments. CX43 interacts with Cav3, suggesting that Cav3 may participate in the functional regulation of GJs.
authors: Department of Cardiology, The First Affiliated Hospital, Shantou University Medical College, 57 Changping Road, 515041, Shantou, People's Republic of China.
authors: Limei
authors: L
authors: Liu
authors: Yuguang
authors: Y
authors: Li
authors: Jijin
authors: J
authors: Lin
authors: Qing
authors: Q
authors: Liang
authors: Xiuzhang
authors: X
authors: Sheng
authors: Jun
authors: J
authors: Wu
authors: Ruiyan
authors: R
authors: Huang
authors: Shukai
authors: S
authors: Liu
authors: Yanyan
authors: Y
authors: Li
mesh_terms: D001483:Base Sequence; D051244:Caveolin 3 / Q000378:metabolism*; D018031:Connexin 43 / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D049452:Green Fluorescent Proteins / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D047468:Immunoprecipitation; D008969:Molecular Sequence Data; D009206:Myocardium / Q000378:metabolism*; D010957:Plasmids / Q000235:genetics; D011485:Protein Binding; D021381:Protein Transport; D020798:Two-Hybrid System Techniques
chemical_list: D051244:Caveolin 3; D018031:Connexin 43; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins