Conformational recovery and preservation of protein nature from heat-induced denaturation by water-soluble phospholipid polymer conjugation.
The effect of water-soluble phospholipid polymer conjugated to a protein on conformational change during heat-quenched stress was investigated in this study. Well-defined pyridine disulfide end-functional poly(2-methacryloyloxyethyl phosphorylcholine) (PMPC) was synthesized by well controlled atom transfer radical polymerization method. Synthesized PMPC was the site specifically conjugated to a protein, and the effect on conformational change during heat-quenched stress was estimated by circular dichroism and fluorescence study. As a result, a single phospholipid polymer chain conjugated to a protein give a great effect on conformational preservation in both secondary and tertiary structures even after the heat-quenched process. Moreover, even the conformational recovery which gave the completely reversible conformational preservation was observed in circular dichroism study. The resulting protein activity was also confirmed, and no significant decline was induced by heat-quenched stress.
Seo
Ji-Hun
JH
Department of Materials Engineering, School of Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.
Matsuno
Ryosuke
R
Lee
Yan
Y
Takai
Madoka
M
Ishihara
Kazuhiko
K
D000818:Animals; D002417:Cattle; D004220:Disulfides / Q000737:chemistry; D006358:Hot Temperature; D008689:Methacrylates / Q000737:chemistry*; D009113:Muramidase / Q000737:chemistry* / Q000378:metabolism; D010767:Phosphorylcholine / Q000031:analogs & derivatives* / Q000737:chemistry; D011109:Polymethacrylic Acids; D011487:Protein Conformation; D011489:Protein Denaturation; D011725:Pyridines / Q000737:chemistry; D012710:Serum Albumin, Bovine / Q000737:chemistry* / Q000378:metabolism
D004220:Disulfides; D008689:Methacrylates; D011109:Polymethacrylic Acids; D011725:Pyridines; C115766:poly(2-methacryloyloxyethyl-phosphorylcholine); D010767:Phosphorylcholine; D012710:Serum Albumin, Bovine; D009113:Muramidase