Variable levels of 37-kDa/67-kDa laminin receptor (RPSA) mRNA in ovine tissues: potential contribution to the regulatory processes of PrPSc propagation?
The 37-kDa laminin receptor precursor/67-kDa laminin receptor (LRP/LR, also known as ribosomal protein SA, RPSA) has been reported to be involved in cancer development and prion internalization. Previous studies have shown that the LRP/LR is expressed in a wide variety of tissues. In particular, expression of LRP/LR mRNA may be closely related to the degree of PrP(Sc) propagation. This study presents a detailed investigation of the LRP/LR mRNA expression levels in eleven normal ovine tissues. Using real-time quantitative PCR, the highest LRP/LR expression was found in neocortex (p < 0.05). Slightly lower levels were found in the heart and obex. Intermediate levels were seen in hippocampus, cerebellum, spleen, thalamus, mesenteric lymph node, and the lowest levels were present in liver, kidney, and lung. In general, the LRP/LR mRNA levels were much higher in neuronal tissues than in peripheral tissues. The observation that differences in LRP/LR mRNA expression levels are consistent with the corresponding variation in PrP(Sc) accumulation suggests that the 37-kDa/67-kDa laminin receptor may be involved in the regulation of PrP(Sc) propagation.
Qiao
Jun-Wen
JW
National Animal TSE Laboratory, College of Veterinary Medicine, China Agriculture University, Beijing, P.R. China.
Su
Xiao-Ou
XO
Li
Yu-Xing
YX
Yang
Jian-Min
JM
Wang
Yi-Qin
YQ
Kouadir
Mohammed
M
Zhou
Xiang-Mei
XM
Yang
Li-Feng
LF
Yin
Xiao-Min
XM
Zhao
De-Ming
DM
D000818:Animals; D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics; D008970:Molecular Weight; D018620:PrPSc Proteins / Q000235:genetics / Q000378:metabolism*; D011498:Protein Precursors / Q000737:chemistry / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics* / Q000378:metabolism*; D017474:Receptors, Laminin / Q000737:chemistry / Q000235:genetics*; D012269:Ribosomal Proteins / Q000737:chemistry / Q000235:genetics; D012608:Scrapie / Q000209:etiology / Q000235:genetics / Q000378:metabolism; D012756:Sheep / Q000235:genetics* / Q000378:metabolism*; D014018:Tissue Distribution
D017931:DNA Primers; D018620:PrPSc Proteins; D011498:Protein Precursors; D012333:RNA, Messenger; D017474:Receptors, Laminin; D012269:Ribosomal Proteins