title: [Application of a simple method for the detection of measles virus genome by loop-mediated isothermal amplification (LAMP)].
abstract: OBJECTIVE
  A new simple RT-LAMP method was applied to detect measles virus nucleic acid and compared with nest-RT-PCR.
  
  
  METHODS
  Compare the detection rate of the RT-LAMP method with that of nest-RT-PCR by detecting measles virus nucleic acid from measles virus and clinical samples.
  
  
  RESULTS
  The nucleic acid positive rates of all 23 strains of measles virus are all 100% by the two methods. But to the detection of 18 clinical samples which are negative in measles isolation, the nest-RT-LAMP showed 56.52% positive rate of nucleic acid of measles virus and nest-RT-PCR showed 47.83%.
  
  
  CONCLUSIONS
  RT-LAMP is more sensitive than nest-RT-PCR.
authors: Jilin Provincial Center for Disease Control and Prevention, Changchun 130062, China.
authors: Jian-hui
authors: JH
authors: Zhou
authors: Xiang
authors: X
authors: Hou
authors: Chao
authors: C
authors: Chen
authors: Shuang
authors: S
authors: Wang
authors: Xin
authors: X
authors: Chang
authors: Gui-yan
authors: GY
authors: Liu
authors: Zhao-nan
authors: ZN
authors: Wang
authors: Song-tao
authors: ST
authors: Xu
authors: Yi-xin
authors: YX
authors: Ji
authors: Wen-bo
authors: WB
authors: Xu
mesh_terms: D016679:Genome, Viral; D006801:Humans; D008457:Measles / Q000821:virology*; D008459:Measles virus / Q000302:isolation & purification*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*