title: Molecular analysis of the copper-responsive CopRSCD of a pathogenic Pseudomonas fluorescens strain.
abstract: CopRS/CopABCD is one of the known systems that control copper homeostasis in bacteria. Although CopRS/CopABCD homologues are found to exist in Pseudomonas fluorescens, the potential role of this system in P. fluorescens has not been investigated. In this study a genetic cluster, consisting of copR, S, C, and D but lacking copAB, was identified in a pathogenic P. fluorescens strain (TSS) isolated from diseased fish. The copRSCD cluster was demonstrated to be required for full copper resistance and regulated at the transcription level by Cu. Expression of copCD is regulated directly by the two-component response regulator CopR, which also regulates its own expression. Interruption of the regulated expression of copR affected bacterial growth, biofilm formation, and tissue dissemination and survival. A mutant CopR, which lacks the N-terminal signal receiver domain and is constitutively active, was found to have an attenuating effect on bacterial virulence when expressed in TSS. To our knowledge, this is the first report that suggests a link between CopR and bacterial pathogenicity in P. fluorescens.
authors: Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, P. R. China.
authors: Yong-Hua
authors: YH
authors: Hu
authors: Hua-Lei
authors: HL
authors: Wang
authors: Min
authors: M
authors: Zhang
authors: Li
authors: L
authors: Sun
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D018441:Biofilms / Q000187:drug effects; D003300:Copper / Q000378:metabolism* / Q000633:toxicity; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D024881:Drug Resistance, Bacterial; D005393:Fish Diseases / Q000382:microbiology*; D005399:Fishes / Q000382:microbiology; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D026901:Membrane Transport Proteins / Q000235:genetics* / Q000378:metabolism*; D050296:Microbial Viability; D008969:Molecular Sequence Data; D005810:Multigene Family; D011552:Pseudomonas Infections / Q000382:microbiology / Q000662:veterinary*; D011551:Pseudomonas fluorescens / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity*; D017422:Sequence Analysis, DNA; D015398:Signal Transduction; D014774:Virulence
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D026901:Membrane Transport Proteins; D003300:Copper