title: Immobilization of poly (ethylene imine) on poly (L-lactide) promotes MG63 cell proliferation and function.
abstract: Poly (ethylene imine) (PEI) is a polycation widely used for DNA transfection to cells but also applied as primary polycation for layer-by-layer (LBL) assembly of polyelectrolytes. The aim of the present study was to investigate the effect of modification with PEI on the biocompatibility of poly (L-lactide) (PLLA) films. PEI with different molecular weight was immobilized on PLLA by either adsorption or covalent binding. Cell morphologies, immuno-fluorescence staining, cell proliferation by lactate dehydrogenase assay and cell differentiation by alkaline phosphatase assay were utilized to assess the biocompatibility of the modified PLLA using osteoblast cell line MG63. Results revealed that PEI modification remarkably improved cell adhesion, viability, proliferation and function compared with plain PLLA. Hence, PEI-modified PLLA is acceptable as transfection vehicle for engineering of bone and other tissues, or as primary layer to allow LBL assembly to generate biomimetic surface coatings.
authors: Institute of Polymer Science, Zhejiang University, Hangzhou 310027, People's Republic of China. zmliu@zju.edu.cn
authors: Zhen-Mei
authors: ZM
authors: Liu
authors: Soo-Yeon
authors: SY
authors: Lee
authors: Sukhéna
authors: S
authors: Sarun
authors: Dieter
authors: D
authors: Peschel
authors: Thomas
authors: T
authors: Groth
mesh_terms: D000327:Adsorption; D001672:Biocompatible Materials / Q000737:chemistry*; D032701:Biomimetics; D002448:Cell Adhesion; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D002470:Cell Survival; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007097:Imines / Q000737:chemistry*; D007770:L-Lactate Dehydrogenase / Q000737:chemistry; D008856:Microscopy, Fluorescence / Q000379:methods; D008858:Microscopy, Phase-Contrast / Q000379:methods; D008970:Molecular Weight; D010006:Osteoblasts / Q000378:metabolism*; D011091:Polyesters / Q000737:chemistry*; D011095:Polyethylenes / Q000737:chemistry*; D011485:Protein Binding; D013499:Surface Properties
chemical_list: D001672:Biocompatible Materials; D007097:Imines; D011091:Polyesters; D011095:Polyethylenes; C505405:poly(ethylene imine); C033616:poly(lactide); D007770:L-Lactate Dehydrogenase