Surface display of IgG Fc on baculovirus vectors enhances binding to antigen-presenting cells and cell lines expressing Fc receptors.
Recombinant baculoviruses (recBV) were constructed with dual cassettes for constitutive expression of human IgG Fc following infection of insect cells and the structural proteins of hepatitis C virus (core, E1 and E2) following transduction of mammalian cells. The IgG Fc was expressed in insect cells as a fusion protein with the signal sequence and transmembrane region of either the native baculovirus envelope protein gp64 or the human transferrin receptor as a type I or type II integral membrane protein, respectively. The IgG Fc fusion proteins formed functional homodimers on the surface of recBV-infected insect cells and were incorporated into the envelope of recBV particles during egress from the infected cell. Both pseudotyped recBV bound specifically to recombinant soluble FcgammaRIIalpha receptor and to cell lines and antigen-presenting cells expressing Fc receptors (FcRs). These novel baculoviral vectors, which target cells of the immune system that express FcRs, have potential applications for vaccination or gene therapy.
Martyn
John C
JC
The Macfarlane Burnet Institute for Medical Research and Public Health, Melbourne, VIC, Australia.
Cardin
Anthony J
AJ
Wines
Bruce D
BD
Cendron
Angela
A
Li
Shuo
S
Mackenzie
Jason
J
Powell
Maree
M
Gowans
Eric J
EJ
D000818:Animals; D000938:Antigen-Presenting Cells / Q000276:immunology*; D016367:Baculoviridae / Q000235:genetics* / Q000276:immunology*; D002460:Cell Line; D017931:DNA Primers; D005434:Flow Cytometry; D016174:Hepacivirus / Q000235:genetics; D006801:Humans; D007141:Immunoglobulin Fc Fragments / Q000235:genetics* / Q000276:immunology; D007074:Immunoglobulin G / Q000235:genetics*; D007313:Insecta / Q000821:virology; D010957:Plasmids / Q000235:genetics; D011961:Receptors, Fc / Q000276:immunology*; D011990:Receptors, Transferrin / Q000235:genetics*; D011993:Recombinant Fusion Proteins / Q000276:immunology; D018411:Spodoptera / Q000276:immunology; D015678:Viral Structural Proteins / Q000235:genetics
D017931:DNA Primers; D007141:Immunoglobulin Fc Fragments; D007074:Immunoglobulin G; D011961:Receptors, Fc; D011990:Receptors, Transferrin; D011993:Recombinant Fusion Proteins; D015678:Viral Structural Proteins