Expression profiling of the genes responding to zearalenone and its analogues using estrogen-responsive genes.
To compare gene expression profiles in response to estrogen or 17beta-estradiol (E(2)) and a mycotoxin, zearalenone (ZEA), and its analogues (collectively termed ZEA compounds), breast cancer MCF-7 cells were treated with 10 nM of E(2) or ZEA compounds including ZEA, alpha-zearalenol, beta-zearalenol, zearalanone, alpha-zearalanol and beta-zearalanol. Expression profiles for 120 estrogen-responsive genes were subjected to cluster and statistical analyses using correlation coefficients or R-values. We found that all of the ZEA compounds stimulated the growth of MCF-7 cells, as much as E(2), and showed similar expression profiles to that of E(2) (R-values ranged from 0.82 to 0.96). The effect of ZEA compounds was likely mediated by estrogen-receptor-dependent Erk1/2-signaling. These results provide clues to understand the mechanism of their estrogen-like action.
Parveen
Meher
M
Signaling Molecules Research Group, Neuroscience Research Institute, National Institute of Advanced Industrial Science and Technology, Tsukuba, Ibaraki, Japan.
Zhu
Yun
Y
Kiyama
Ryoiti
R
D001943:Breast Neoplasms / Q000378:metabolism; D045744:Cell Line, Tumor / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004789:Enzyme Activation; D004958:Estradiol / Q000494:pharmacology; D004967:Estrogens / Q000494:pharmacology; D004968:Estrogens, Non-Steroidal / Q000737:chemistry / Q000494:pharmacology*; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism; D005260:Female; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects*; D006801:Humans; D015394:Molecular Structure; D020411:Oligonucleotide Array Sequence Analysis; D011960:Receptors, Estrogen / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects; D015025:Zearalenone / Q000031:analogs & derivatives / Q000494:pharmacology
D004967:Estrogens; D004968:Estrogens, Non-Steroidal; D011960:Receptors, Estrogen; D004958:Estradiol; D015025:Zearalenone; D048049:Extracellular Signal-Regulated MAP Kinases