In-vivo imaging of murine tumors using complete-angle projection fluorescence molecular tomography.
We interrogate the ability of free-space fluorescence tomography to image small animals in vivo using charge-coupled device (CCD) camera measurements over 360-deg noncontact projections. We demonstrate the performance of normalized dual-wavelength measurements that are essential for in-vivo use, as they account for the heterogeneous distribution of photons in tissue. In-vivo imaging is then showcased on mouse lung and brain tumors cross-validated by x-ray microcomputed tomography and histology.
Deliolanis
Nikolaos C
NC
Dunham
Joshua
J
Wurdinger
Thomas
T
Figueiredo
Jose-Luiz
JL
Tannous
Bakhos A
BA
Bakhos
Tannous
T
Ntziachristos
Vasilis
V
D000818:Animals; D001932:Brain Neoplasms / Q000175:diagnosis / Q000473:pathology*; D005453:Fluorescence; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D008175:Lung Neoplasms / Q000175:diagnosis / Q000473:pathology*; D051379:Mice; D015203:Reproducibility of Results; D014054:Tomography / Q000379:methods*; D055114:X-Ray Microtomography