Rapid and dynamic intracellular patterning of cell-internalized magnetic fluorescent nanoparticles.
Conjugated magnetic nanoparticles have recently demonstrated potential in activating unique and specific activity within cells. Leveraging microfabrication, we have developed a technique of localizing nanoparticles to specific, subcellular locations by a micropatterned ferromagnetic substrate. Controlled patterns of nanoparticles were assembled and dynamically controlled with submicrometer precision within live cells. We anticipate that the technique will be useful as a compact, simple method of generating localizable, subcellular chemical and mechanical signals, compatible with standard microscopy.
Tseng
Peter
P
Department of Electrical Engineering, California Nanosystems Institute, University of California, Los Angeles, Los Angeles, California, USA. ptseng@ucla.edu
Di Carlo
Dino
D
Judy
Jack W
JW
D002478:Cells, Cultured; D008280:Magnetics; D008856:Microscopy, Fluorescence; D053758:Nanoparticles / Q000737:chemistry*; D013499:Surface Properties; D013997:Time Factors