Effect of coenzyme Q10 on cutaneous healing in skin-incised mice.
Coenzyme Q10 (CoQ10) is a biosynthesized quinone with 10 isoprene side chains in humans. To investigate the anti-inflammatory and wound healing effect of CoQ10, we performed in vivo and in vitro experiments. In vivo studies, there were 3 groups; Naive (without skin incision), Control (with skin incision) and CoQ10 (100 mg/kg treatment with skin incision). Collagen-like polymer (CLP) level of CoQ10 group was increased significantly compared to the control group (p<0.05). Also, CoQ10 group showed significant inhibition on myeloperoxidase (MPO) and PLA(2) level compared to the control group (p<0.05). These data show that CoQ10 may have an anti-inflammatory and a wound healing effect. CoQ10 showed significant antioxidant activity in vivo on malondialdehyde (MDA) and superoxide dismutase (SOD) levels compared to the control group (p<0.05). Although CoQ10 did not show antioxidant activity in cell free system of DPPH radical scavenge, it had a potent antioxidant activity in cell culture system of both silica- and zymosan-induced reactive oxygen species generation using Raw 264.7 cells. This result may be associated with the conversion of CoQ10 to the reduced form (CoQ10H(2)) in the presence of some kinds of intracellular reducing agents. In conclusion, it is considered that CoQ10 appears to have a cutaneous healing effect in vivo, which may be related to the secondary action of CoQ10.
Choi
Bang Shil
BS
Department of Pathophysiology, College of Pharmacy, Chung-Ang University, Seoul 156-756, Korea.
Song
Ho Sun
HS
Kim
Hee Rae
HR
Park
Tae Wook
TW
Kim
Tae Doo
TD
Cho
Bong Jae
BJ
Kim
Chang Jong
CJ
Sim
Sang Soo
SS
D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology* / Q000627:therapeutic use*; D002460:Cell Line; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D009195:Peroxidase / Q000378:metabolism; D054467:Phospholipases A2 / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000037:antagonists & inhibitors; D012867:Skin / Q000293:injuries* / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism; D014451:Ubiquinone / Q000031:analogs & derivatives* / Q000494:pharmacology / Q000627:therapeutic use; D014945:Wound Healing / Q000187:drug effects*
D000893:Anti-Inflammatory Agents; D017382:Reactive Oxygen Species; D014451:Ubiquinone; D008315:Malondialdehyde; D009195:Peroxidase; D013482:Superoxide Dismutase; D054467:Phospholipases A2; C024989:coenzyme Q10