title: Human decidual stromal cells protect lymphocytes from apoptosis.
abstract: Human decidual stromal cells (DSC) have been shown to be involved in different immune functions that may be relevant for the relationship between the mother and fetus and hence for successful pregnancy. The expression of death ligands by fetal trophoblast and maternal decidual cells has been proposed as a mechanism for the establishment of materno-fetal immunotolerance. This study intended to elucidate the interrelations between DSC and lymphocytes. We analyzed the expression and function of death receptors and ligands in DSC maintained in culture. These DSC lines expressed CD95 and TNF-related apoptosis-inducing ligand receptor-2 (TRAIL-R2), although they were resistant to death receptor-mediated apoptosis. Regarding the expression of CD95L and TRAIL, it was variable among DSC lines although none of them induced apoptosis in death ligand-sensitive Jurkat T cells. Interestingly, most of the DSC lines, as well as fresh DSC, reduced apoptosis in Jurkat cells induced by anti-CD95 antibody and recombinant TRAIL. The protective effect of DSC was observed when they were co-cultured with Jurkat cells in Transwell plates, indicating that DSC may produce soluble factors of importance for lymphocyte survival. Moreover, the viability of peripheral blood lymphocytes and decidual lymphocytes was improved when co-cultured with DSC. Our results suggest that DSC, far from inducing apoptosis, may be relevant in the regulation of lymphocyte survival at the materno-fetal interface.
authors: Unidad de Inmunología, IBIMER, Universidad de Granada, Centro de Investigación Biomédica, Avda. del Conocimiento s/n, 18100 Armilla, Granada, Spain.
authors: O
authors: O
authors: Blanco
authors: E
authors: E
authors: Leno-Durán
authors: J C
authors: JC
authors: Morales
authors: E G
authors: EG
authors: Olivares
authors: C
authors: C
authors: Ruiz-Ruiz
mesh_terms: D017209:Apoptosis / Q000187:drug effects / Q000276:immunology*; D002460:Cell Line; D002470:Cell Survival / Q000276:immunology; D018920:Coculture Techniques; D003656:Decidua / Q000166:cytology* / Q000276:immunology*; D004317:Doxorubicin / Q000494:pharmacology; D053222:Fas Ligand Protein / Q000378:metabolism; D005260:Female; D006801:Humans; D007108:Immune Tolerance; D019169:Jurkat Cells; D008214:Lymphocytes / Q000166:cytology* / Q000187:drug effects / Q000276:immunology*; D008431:Maternal-Fetal Exchange / Q000276:immunology*; D011247:Pregnancy; D053220:Receptors, TNF-Related Apoptosis-Inducing Ligand / Q000378:metabolism; D017154:Stromal Cells / Q000166:cytology* / Q000276:immunology*; D014635:Valproic Acid / Q000494:pharmacology
chemical_list: D053222:Fas Ligand Protein; D053220:Receptors, TNF-Related Apoptosis-Inducing Ligand; D014635:Valproic Acid; D004317:Doxorubicin