[Antibody detection and sequencing analysis of hepatitis E virus in human population, swine and chicken in Sichuan region in China].
OBJECTIVE
To investigate the seroprevalence of HEV infection and genotype.


METHODS
ELISA were used for detecting anti-HEV IgG of the serum samples, the nested reverse transcriptase PCR (RT-nPCR) was used for detecting HEV RNA in patient serum and swine bile samples. All samples were collected in 2005-2007 in some districts in Sichuan province. The primers used for genotyping were the ORF2 region of HEV genome.


RESULTS
The anti-HEV IgG was detected positive in childrens 6.10% (41/672), adults 42.26% (280/ 661), swines 88.89% (32/36), chickens negative (0/59). 1 case of 15 serum samples of anti-HEV IgM positive and 3 of 54 swine bile samples were positive for HEV RNA by RT-PCR.Sequence analysis of 4 isolates has 92.1% to 98.6% nucleotide sequence homology. These isolates from human and swine were identified closely related to Ch-T21 strain 90.1%-96.9% sequence homology, which belonged to HEV genotype 4B.


CONCLUSIONS
The swine were the risk factors in the spread of hepatitis E virus.
Jiang
Yong-zhen
YZ
National Institute for Viral Disease Control and Prevention, China CDC, Beijing 100052, China. jiangyz1115@sina.com
Lu
Jian
J
Zhang
Li-ping
LP
Tian
Rui-guang
RG
Liu
Qing-lian
QL
Bi
Sheng-li
SL
D000818:Animals; D002645:Chickens; D002648:Child; D002675:Child, Preschool; D002681:China; D004797:Enzyme-Linked Immunosorbent Assay; D005838:Genotype; D006508:Hepatitis Antibodies / Q000276:immunology*; D016751:Hepatitis E / Q000145:classification / Q000453:epidemiology* / Q000235:genetics; D016752:Hepatitis E virus / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D006801:Humans; D010802:Phylogeny; D016036:Seroepidemiologic Studies; D013552:Swine
D006508:Hepatitis Antibodies