title: [Role of erythrocyte cytoplasmic structures in changes in the affinity of haemoglobin for oxygen].
abstract: Changes in the refractive index of the cytoplasm and the affinity of haemoporphyrin of erythrocyte haemoglobin to oxygen (pH, 2,3-diphosphoglycerate) have been investigated using laser interference microscopy and Raman spectroscopy. It has been established that a decrease in pH and an increase in the content of 2,3-diphosphoglycerate are accompanied by changes in both the form of the cell and the refractive index of the cytoplasm and the affinity of haemoporphyrin of hemoglobin to oxygen. It has been shown that as pH is reduced, the capacity of haemoporphyrin for binding oxygen decreases and as the concentration of 2,3-diphosphoglycerate is increased, the ability of haemoporphyrin for oxygen reabsorption increases.
authors: N Iu
authors: NIu
authors: Bryzgalova
authors: N A
authors: NA
authors: Brazhe
authors: A U
authors: AU
authors: Iusipovich
authors: G V
authors: GV
authors: Maksimov
authors: A B
authors: AB
authors: Rubin
mesh_terms: D019794:2,3-Diphosphoglycerate / Q000494:pharmacology; D048430:Cell Shape; D003593:Cytoplasm / Q000648:ultrastructure*; D004912:Erythrocytes / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D045604:Extracellular Fluid / Q000737:chemistry; D006415:Hematoporphyrins / Q000737:chemistry / Q000378:metabolism; D006454:Hemoglobins / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D018613:Microscopy, Confocal; D008857:Microscopy, Interference; D010100:Oxygen / Q000378:metabolism*; D011487:Protein Conformation; D013059:Spectrum Analysis, Raman
chemical_list: D006415:Hematoporphyrins; D006454:Hemoglobins; D019794:2,3-Diphosphoglycerate; D010100:Oxygen