Discriminating foot-and-mouth disease virus-infected and vaccinated animals by use of beta-galactosidase allosteric biosensors.
Recombinant beta-galactosidases accommodating one or two different peptides from the foot-and-mouth disease virus (FMDV) nonstructural protein 3B per enzyme monomer showed a drastic enzymatic activity reduction, which mainly affected proteins with double insertions. Recombinant beta-galactosidases were enzymatically reactivated by 3B-specific murine monoclonal and rabbit polyclonal antibodies. Interestingly, these recombinant beta-galactosidases, particularly those including one copy of each of the two 3B sequences, were efficiently reactivated by sera from infected pigs. We found reaction conditions that allowed differentiation between sera of FMDV-infected pigs, cattle, and sheep and those of naïve and conventionally vaccinated animals. These FMDV infection-specific biosensors can provide an effective and versatile alternative for the serological distinction of FMDV-infected animals.
Sánchez-Aparicio
M Teresa
MT
Centro de Biología Molecular Severo Ochoa (CSIC-UAM), Universidad Autónoma de Madrid, Cantoblanco, Madrid, Spain.
Rosas
María Flora
MF
Ferraz
Rosa Maria
RM
Delgui
Laura
L
Veloso
Juan J
JJ
Blanco
Esther
E
Villaverde
Antonio
A
Sobrino
Francisco
F
D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology; D015374:Biosensing Techniques / Q000379:methods*; D002417:Cattle; D002418:Cattle Diseases / Q000175:diagnosis / Q000276:immunology; D003937:Diagnosis, Differential; D005536:Foot-and-Mouth Disease / Q000175:diagnosis* / Q000276:immunology*; D029544:Foot-and-Mouth Disease Virus / Q000276:immunology*; D051379:Mice; D011817:Rabbits; D012756:Sheep; D012757:Sheep Diseases / Q000175:diagnosis / Q000276:immunology; D013552:Swine; D013553:Swine Diseases / Q000175:diagnosis / Q000276:immunology; D014765:Viral Vaccines / Q000276:immunology*; D001616:beta-Galactosidase / Q000378:metabolism*
D000914:Antibodies, Viral; D014765:Viral Vaccines; D001616:beta-Galactosidase