Stability evaluation of temoporfin-loaded liposomal gels for topical application.
Temoporfin (mTHPC) is a potent second-generation synthetic photosensitizer. Topical delivery of mTHPC is of great interest for the photodynamic therapy of psoriasis and superficial skin cancer lesions. The aim of this study was to evaluate the stability of hydrophilic gels containing mTHPC-loaded liposomes. Two different mTHPC-loaded liposome dispersions, composed of 15 % (w/w) nonhydrogenated soybean lecithin of different phosphatidylcholine content, were prepared and incorporated (2:1 w/w) into hydrogels of different carbomer concentrations (1.5, 2.25, and 3%; w/w). Obtained liposomal hydrogels, containing 0.15% (w/w) mTHPC, 10% (w/w) phospholipids, and 0, 0.5, or 1% (w/w) carbomer, were analyzed for flow properties, liposome particle size, and polydispersity index (PDI), pH value, and mTHPC content after their preparation and at predetermined time intervals during 6 months of storage at 4 and 23 degrees C. All hydrogels showed, during the whole period of investigation, adequate characteristics for topical application (i.e., they revealed shear-thinning plastic flow behavior). Rheological parameters, particle size, and PDI of liposomes in hydrogels, mTHPC content, and pH value did not show remarkable changes during the storage of gels, which could make them unacceptable for topical use. The obtained results indicated physical and chemical stability of liposomal gels containing mTHPC during 6 months of storage at both temperatures.
Dragicevic-Curic
Nina
N
Department of Pharmaceutical Technology, Friedrich Schiller University Jena, Jena, Germany. ninadragicevic@hotmail.com
Winter
Sven
S
Krajisnik
Danina
D
Stupar
Mirjana
M
Milic
Jela
J
Graefe
Susanna
S
Fahr
Alfred
A
D000180:Acrylic Resins; D000287:Administration, Topical; D005782:Gels; D020100:Hydrogels / Q000737:chemistry; D008081:Liposomes / Q000737:chemistry*; D008652:Mesoporphyrins; D010316:Particle Size; D010713:Phosphatidylcholines; D010778:Photochemotherapy; D017319:Photosensitizing Agents / Q000008:administration & dosage / Q000737:chemistry; D012212:Rheology
D000180:Acrylic Resins; D005782:Gels; D020100:Hydrogels; D008081:Liposomes; D008652:Mesoporphyrins; D010713:Phosphatidylcholines; D017319:Photosensitizing Agents; C479038:carbomer; C072269:temoporfin