title: Alterations of the synovial T cell repertoire in anti-citrullinated protein antibody-positive rheumatoid arthritis.
abstract: OBJECTIVE
  The association of HLA-DRB1 alleles with anti-citrullinated protein antibodies (ACPAs) in rheumatoid arthritis (RA) suggests the potential involvement of T lymphocytes in ACPA-seropositive disease. The purpose of this study was to investigate this hypothesis by systematic histologic and molecular analyses of synovial T cells in ACPA+ versus ACPA- RA patients.
  
  
  METHODS
  Synovial biopsy samples were obtained from 158 RA patients. Inflammation was determined histologically and immunohistochemically. RNA was extracted from peripheral blood mononuclear cells and synovial tissues obtained from 11 ACPA+ RA patients, 7 ACPA- RA patients, and 10 spondylarthritis (SpA) patients (arthritis controls). T lymphocyte clonality was studied by combined quantitative and qualitative T cell receptor CDR3 length distribution (LD) analysis and direct sequencing analysis.
  
  
  RESULTS
  ACPA+ and ACPA- RA patients were similar at both the clinical and histologic levels. At the molecular level, however, patients with ACPA+ synovitis displayed a marked elevation of qualitative CDR3 LD alterations as compared with those with ACPA- synovitis and with the SpA controls. These differences in CDR3 LD were not observed in the peripheral blood, indicating a selective recruitment and/or local expansion of T cells in the synovial compartment. The CDR3 LD alterations reflected true monoclonal or oligoclonal expansions, as confirmed by direct sequencing of the T cell receptor. The CDR3 LD alterations in RA synovium did not correlate with B cell clonal expansions but were inversely associated with synovial lymphoid neogenesis.
  
  
  CONCLUSIONS
  The T cell repertoire is specifically restricted in RA patients with ACPA+ synovitis. Whereas the origin and role of these clonal alterations remain to be determined, our data suggest the preferential involvement of T lymphocytes in ACPA-seropositive RA.
authors: Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands.
authors: Tineke
authors: T
authors: Cantaert
authors: Sophie
authors: S
authors: Brouard
authors: Rogier M
authors: RM
authors: Thurlings
authors: Annaick
authors: A
authors: Pallier
authors: Gabriela Franco
authors: GF
authors: Salinas
authors: Christophe
authors: C
authors: Braud
authors: Paul L
authors: PL
authors: Klarenbeek
authors: Niek
authors: N
authors: de Vries
authors: Yiping
authors: Y
authors: Zhang
authors: Jean-Paul
authors: JP
authors: Soulillou
authors: Paul P
authors: PP
authors: Tak
authors: Dominique
authors: D
authors: Baeten
mesh_terms: D000328:Adult; D000368:Aged; D000483:Alleles; D000888:Antibodies, Anti-Idiotypic / Q000378:metabolism*; D001172:Arthritis, Rheumatoid / Q000276:immunology* / Q000378:metabolism / Q000473:pathology*; D001483:Base Sequence; D001706:Biopsy; D016022:Case-Control Studies; D002465:Cell Movement; D022801:Complementarity Determining Regions / Q000235:genetics / Q000378:metabolism; D005260:Female; D006684:HLA-DR Antigens / Q000235:genetics / Q000378:metabolism; D059811:HLA-DRB1 Chains; D006801:Humans; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D010456:Peptides, Cyclic / Q000276:immunology*; D011948:Receptors, Antigen, T-Cell / Q000235:genetics / Q000378:metabolism; D025241:Spondylarthritis / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D013583:Synovial Membrane / Q000378:metabolism / Q000473:pathology*; D013585:Synovitis / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D013601:T-Lymphocytes / Q000378:metabolism / Q000473:pathology*
chemical_list: D000888:Antibodies, Anti-Idiotypic; D022801:Complementarity Determining Regions; D006684:HLA-DR Antigens; D059811:HLA-DRB1 Chains; D010456:Peptides, Cyclic; D011948:Receptors, Antigen, T-Cell; C487763:cyclic citrullinated peptide