title: Antibacterial activity of recombinant hCAP18/LL37 protein secreted from Pichia pastoris.
abstract: Human antimicrobial peptide CAP18/LL37 (hCAP18/LL37) was expressed in Pichia pastoris and its antibacterial activity was tested against pathogenic bacteria. The full length ORF of hCAP18/LL37 was cloned into the pPICZaA vector followed by integration into the genomic AOX1 gene of P. pastoris. Agar diffusion assay demonstrated that the different hCAP18/LL37 transformants showed various antibacterial activities against Staphylococcus aureus, Micrococcus luteus, and Salmonella gastroenteritis. The secreted form of hCAP18/LL37 exhibited its maximum activity after 72 h incubation with 2% methanol in MM media, not in BMM. This result suggests that the yeast secreted expression system can be used as a production tool of antimicrobial peptides for industrial or pharmaceutical application.
authors: Department of Biochemistry, College of Medicine, Chungbuk National University, Cheongju 361-763, Republic of Korea. hankyol2@hanmail.net
authors: Soon-ja
authors: SJ
authors: Kim
authors: Renshu
authors: R
authors: Quan
authors: Sung-Jin
authors: SJ
authors: Lee
authors: Hak-Kyo
authors: HK
authors: Lee
authors: Joong-Kook
authors: JK
authors: Choi
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D023181:Antimicrobial Cationic Peptides / Q000235:genetics / Q000494:pharmacology*; D054804:Cathelicidins; D003470:Culture Media / Q000737:chemistry; D006801:Humans; D008826:Microbial Sensitivity Tests / Q000379:methods; D016982:Micrococcus luteus / Q000187:drug effects*; D010843:Pichia / Q000235:genetics; D011994:Recombinant Proteins / Q000235:genetics / Q000494:pharmacology; D012475:Salmonella / Q000187:drug effects*; D013211:Staphylococcus aureus / Q000187:drug effects*
chemical_list: D000900:Anti-Bacterial Agents; D023181:Antimicrobial Cationic Peptides; D054804:Cathelicidins; D003470:Culture Media; D011994:Recombinant Proteins