[Effect of hydroquinone on the expression of ubiquitin-conjugating enzyme Rad6B in human L-02 hepatic cells].
OBJECTIVE
To study the effects of hydroquinone (HQ) on expression of ubiquitin-conjugating enzyme Rad6B in human L-02 hepatic cells.


METHODS
After L-02 hepatic cells were at the concentrations of 0, 5, 10, 20, 40, 80, and 160 micromol/L HQ for 24h, and the levels of Rad6B mRNA and protein expressions in L-02 hepatic cells were detected by real-time fluorescent quantitative polymerase chain reaction (QPCR) technique and Western blot method respectively.


RESULTS
It was found that HQ in the range of 0-160 micromol/L could induce increases in the expressions of Rad6B mRNA and protein, which was in a dose-dependent manner. The levels of Rad6B mRNA and protein expressions in the treated group of 5, 10, 20, 40, 80, and 160 micromol/L were higher than those in the control (P < 0.01), levels of Rad6B mRNA and protein expressions reached the maximun when L-02 hepatic cells were treated by HQ at the concentrations of 160 micromol/L, and the relative quantity values were 4.35 and 0.85, respectively.


CONCLUSIONS
HQ could regulate up the expression of Rad6B in L-02 hepatic cells.
Hu
Gonghua
G
Department of Preventive Medicine, Gannan Medical College, Ganzhou 341000, China. hgh0129@sohu.com
Zhuang
Zhixiong
Z
Yang
Jianping
J
Huang
Haiyan
H
D002460:Cell Line; D022781:Hepatocytes / Q000166:cytology / Q000201:enzymology*; D006801:Humans; D006873:Hydroquinones / Q000633:toxicity*; D009153:Mutagens / Q000633:toxicity; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D044763:Ubiquitin-Conjugating Enzymes / Q000235:genetics / Q000378:metabolism*; D015854:Up-Regulation / Q000187:drug effects
D006873:Hydroquinones; D009153:Mutagens; D012333:RNA, Messenger; C471546:UBE2B protein, human; D044763:Ubiquitin-Conjugating Enzymes; C031927:hydroquinone