[Experimental verification of a model of complement-dependent immune lysis of liposomes].
The quantitative dependences of the immune complement-dependent lysis of a monodisperse suspension of 200-nm liposomes whose membrane was sensitized by monovalent hapten (2,4-DNP-epsilon-caproyl-DPPE) or a polyvalent antigen (LPS from F. tularensis) on the initial concentration of specific antibodies (IgG) to hapten and the antigen have been investigated. The quantity of antibodies binding sites on the surface of liposome was evaluated. The difference between the complement-dependent immune lysis of poly- and monodisperse suspensions of liposomes was shown. The experimental results are well described by the direct binding model.
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D000911:Antibodies, Monoclonal / Q000737:chemistry; D000936:Antigen-Antibody Complex / Q000737:chemistry*; D002784:Cholesterol / Q000737:chemistry; D003165:Complement System Proteins / Q000737:chemistry*; D005604:Francisella tularensis / Q000737:chemistry; D006241:Haptens / Q000737:chemistry; D007074:Immunoglobulin G / Q000737:chemistry; D054709:Lecithins / Q000737:chemistry; D008070:Lipopolysaccharides / Q000737:chemistry; D010755:Organophosphates / Q000737:chemistry; D010714:Phosphatidylethanolamines / Q000737:chemistry / Q000276:immunology; D053835:Unilamellar Liposomes / Q000737:chemistry* / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex; D006241:Haptens; D007074:Immunoglobulin G; D054709:Lecithins; D008070:Lipopolysaccharides; D010755:Organophosphates; D010714:Phosphatidylethanolamines; D053835:Unilamellar Liposomes; C002290:dicetylphosphate; C033368:N-(2,4,6-trinitrophenyl-6-N-aminocaproyl)-1,2-dipalmitoylphosphatidylethanolamine; D003165:Complement System Proteins; D002784:Cholesterol