[Protective effect of Buyanghuanwu Decoction on myocardial ischemia induced by isoproterenol in rats].
OBJECTIVE
To study the protective effect of Buyanghuanwu Decoction on myocardial ischemia induced by isoproterenol in rats.


METHODS
Buyanghuanwu Decoction was given in different dose and the rat model of myocardial ischemia was established by peritoneal injection of isoproterenol. The expression of CD40 in whole blood was detected by flow cytometry,and the expression of CD40L in myocardial tissues was detected by immunohistochemistry. The activities of lactate dehydrogenase (LDH), creatine kinase (CK) and aspartate aminotransferase (AST) in blood serum were detected by biochemistry detector.


RESULTS
Compared with the model group, Buyanghuanwu Decoction in high and middle dose significantly inhibited the expression of CD40 in blood serum and CD40L in myocardial tissues (P < 0.01), and obviously decreased the activities of LDH, CK and AST in blood serum (P < 0.01).


CONCLUSIONS
Buyanghuanwu Decoction has a protective effect on myocardial ischemia induced by isoproterenol in rats, and it may be relevant to the decrease of the expression of CD40-CD40L and the activities of myocardial enzymes.
Liu
Yu
Y
Department of Pharmacology, Medicine School of Xi'an Jiaotong University, Xi'an 710061, China.
Lin
Rong
R
Zhang
Hui
H
Zhang
Ji-Ye
JY
Ji
Qiao-Li
QL
Yang
Yin-Jing
YJ
D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood; D019013:CD40 Antigens / Q000097:blood; D023201:CD40 Ligand / Q000378:metabolism; D002316:Cardiotonic Agents / Q000494:pharmacology*; D003402:Creatine Kinase / Q000097:blood; D004195:Disease Models, Animal; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000302:isolation & purification / Q000494:pharmacology*; D007150:Immunohistochemistry; D007267:Injections; D007545:Isoproterenol; D007770:L-Lactate Dehydrogenase / Q000097:blood; D008297:Male; D017202:Myocardial Ischemia / Q000139:chemically induced / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*; D009206:Myocardium / Q000378:metabolism* / Q000473:pathology; D010946:Plants, Medicinal / Q000737:chemistry; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley
D019013:CD40 Antigens; D002316:Cardiotonic Agents; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; D023201:CD40 Ligand; D007770:L-Lactate Dehydrogenase; D001219:Aspartate Aminotransferases; D003402:Creatine Kinase; D007545:Isoproterenol