Protein kinases A and C in post-mortem prefrontal cortex from persons with major depression and normal controls.
Major depression (MDD) is a common and potentially life-threatening condition. Widespread neurobiological abnormalities suggest abnormalities in fundamental cellular mechanisms as possible physiological mediators. Cyclic AMP-dependent protein kinase [also known as protein kinase A (PKA)] and protein kinase C (PKC) are important components of intracellular signal transduction cascades that are linked to G-coupled receptors. Previous research using both human peripheral and post-mortem brain tissue specimens suggests that a subset of depressed patients exhibit reduced PKA and PKC activity, which has been associated with reduced levels of specific protein isoforms. Prior research also suggests that specific clinical phenotypes, particularly melancholia and suicide, may be particularly associated with low activity. This study examined PKA and PKC protein levels in human post-mortem brain tissue samples from persons with MDD (n=20) and age- and sex-matched controls (n=20). Specific PKA subunits and PKC isoforms were assessed using Western blot analysis in post-mortem samples from Brodmann area 10, which has been implicated in reinforcement and reward mechanisms. The MDD sample exhibited significantly lower protein expression of PKA regulatory Ialpha (RIalpha), PKA catalytic alpha (Calpha) and Cbeta, PKCbeta1, and PKCepsilon relative to controls. The melancholic subgroup showed low PKA RIalpha and PKA Cbeta, while the portion of the MDD sample who died by suicide had low PKA RIalpha and PKA Calpha. These data continue to support the significance of abnormalities of these two key kinases, and suggest linkages between molecular endophenotypes and specific clinical phenotypes.
Shelton
Richard C
RC
Department of Psychiatry, Vanderbilt University, Nashville, TN 37212, USA. richard.shelton@vanderbilt.edu
Hal Manier
D
D
Lewis
David A
DA
D000328:Adult; D000368:Aged; D001344:Autopsy; D015153:Blotting, Western; D016022:Case-Control Studies; D054751:Cyclic AMP-Dependent Protein Kinase Catalytic Subunits / Q000032:analysis; D054756:Cyclic AMP-Dependent Protein Kinase RIalpha Subunit / Q000032:analysis; D017868:Cyclic AMP-Dependent Protein Kinases / Q000032:analysis*; D003865:Major Depressive Disorder / Q000201:enzymology* / Q000523:psychology; D015536:Down-Regulation; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D010641:Phenotype; D017397:Prefrontal Cortex / Q000201:enzymology*; D011493:Protein Kinase C / Q000032:analysis*; D064546:Protein Kinase C beta; D051744:Protein Kinase C-epsilon / Q000032:analysis; D013405:Suicide; D055815:Young Adult
D054756:Cyclic AMP-Dependent Protein Kinase RIalpha Subunit; C517834:PRKAR1A protein, human; D054751:Cyclic AMP-Dependent Protein Kinase Catalytic Subunits; D017868:Cyclic AMP-Dependent Protein Kinases; C517757:PRKACA protein, human; C517760:PRKACB protein, human; C497255:PRKCE protein, human; D011493:Protein Kinase C; D064546:Protein Kinase C beta; D051744:Protein Kinase C-epsilon