Localization of D-β-aspartyl residue-containing proteins in various tissues.
Prior to the emergence of life, it is believed that only l-amino acids were selected for formation of protein and that d-amino acids were eliminated on the primitive Earth. Whilst homochirality is essential for life, the occurrence of proteins containing d-beta-aspartyl (Asp) residues in various tissues from elderly subjects has been reported recently. Here, we demonstrate the presence of a d-beta-Asp-containing protein in the cardiac muscle of heart, blood vessels of the lung, chief cells of the stomach, longitudinal and circular muscle of the stomach, small intestine and large intestine. Since the d-beta-Asp residue occurs through a succinimide intermediate, this isomer may potentially be generated in proteins more easily than initially thought. Formation of the d-beta-Asp residue in proteins may be related to stress.
Motoie
Ryota
R
Research Reactor Institute, Kyoto University, Kumatori, Sennan, Osaka 590-0494, Japan.
Fujii
Noriko
N
Research Reactor Institute, Kyoto University, Kumatori, Sennan, Osaka 590-0494, Japan.
Tsunoda
Shigeru
S
Graduate School of Science, Osaka Prefecture University, Sakai, Osaka 599-8531, Japan.
Nagata
Kenji
K
Research Reactor Institute, Kyoto University, Kumatori, Sennan, Osaka 590-0494, Japan.
Shimo-Oka
Tadashi
T
Life Science Center, Asahi Techno Glass Corp, Funabashi, Chiba 273-0044, Japan.
Kinouchi
Tadatoshi
T
Research Reactor Institute, Kyoto University, Kumatori, Sennan, Osaka 590-0494, Japan.
Fujii
Norihiko
N
Research Reactor Institute, Kyoto University, Kumatori, Sennan, Osaka 590-0494, Japan.
Saito
Takeshi
T
Research Reactor Institute, Kyoto University, Kumatori, Sennan, Osaka 590-0494, Japan.
Ono
Koji
K
Research Reactor Institute, Kyoto University, Kumatori, Sennan, Osaka 590-0494, Japan.
D000818:Animals; D001224:Aspartic Acid / Q000737:chemistry*; D001808:Blood Vessels / Q000378:metabolism; D005260:Female; D005753:Gastric Mucosa / Q000378:metabolism; D007150:Immunohistochemistry; D007420:Intestine, Large / Q000378:metabolism; D007421:Intestine, Small / Q000378:metabolism; D007536:Isomerism; D051379:Mice; D008809:Mice, Inbred C3H; D009206:Myocardium / Q000378:metabolism; D018384:Oxidative Stress; D011506:Proteins / Q000032:analysis / Q000737:chemistry* / Q000378:metabolism
D011506:Proteins; D001224:Aspartic Acid