Patterning of 293T fibroblasts on a mica surface.
Controllable cell growth on the defined areas of surfaces is important for potential applications in biosensor fabrication and tissue engineering. In this study, controllable cell growth was achieved by culturing 293 T fibroblast cells on a mica surface which had been patterned with collagen strips by a microcontact printing (microCP) technique. The collagen area was designed to support cell adhesion and the native mica surface was designed to repel cell adhesion. Consequently, the resulting cell patterns should follow the micro-patterns of the collagen. X-ray photoelectron spectroscopy (XPS), water contact angle (WCA) measurement, atomic-force microscope (AFM) observation, and force-curve measurement were used to monitor property changes before and after the collagen adsorption process. Further data showed that the patterned cells were of good viability and able to perform a gene-transfection experiment in vitro. This technique should be of potential applications in the fields of biosensor fabrication and tissue engineering.
Hou
Sen
S
College of Life Science, Nankai University, Tianjin 300071, China.
Li
Xin-Xin
XX
Li
Xiao-Yu
XY
Feng
Xi-Zeng
XZ
Guan
Li
L
Yang
Yan-Lian
YL
Wang
Chen
C
D000538:Aluminum Silicates / Q000737:chemistry*; D002448:Cell Adhesion; D018929:Cell Culture Techniques / Q000379:methods*; D002460:Cell Line; D002470:Cell Survival; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D003094:Collagen / Q000737:chemistry*; D005347:Fibroblasts / Q000166:cytology*; D006801:Humans; D018625:Microscopy, Atomic Force; D013052:Spectrometry, X-Ray Emission; D014162:Transfection; D017155:Wettability
D000538:Aluminum Silicates; D020099:Coated Materials, Biocompatible; D003094:Collagen; C011934:mica