Self-powered microfluidic chips for multiplexed protein assays from whole blood.
We report herein on a self-powered, self-contained microfluidic-based chip designed to separate plasma from whole blood, and then execute an assay of a multiplexed panel of plasma biomarker proteins. The power source is based upon a chemical reaction that is catalytically triggered by the push of a button on the chip. We demonstrate assays of a dozen blood-based protein biomarkers using this automated, self-contained device. This platform can potentially permit high throughput, accurate, multiplexed blood diagnostic measurements in remote locations and by minimally trained individuals.
Qin
Lidong
L
NanoSystems Biology Cancer Center(NSBCC), Kavli Nanoscience Institute, Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, California 91125, USA.
Vermesh
Ophir
O
Shi
Qihui
Q
Heath
James R
JR
D015415:Biomarkers / Q000097:blood; D001774:Blood Chemical Analysis / Q000295:instrumentation*; D001798:Blood Proteins / Q000032:analysis*; D004129:Dimethylpolysiloxanes / Q000737:chemistry; D006801:Humans; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D009757:Nylons / Q000737:chemistry; D010545:Peroxides / Q000737:chemistry; D011312:Pressure; D012680:Sensitivity and Specificity
D015415:Biomarkers; D001798:Blood Proteins; D004129:Dimethylpolysiloxanes; D009757:Nylons; D010545:Peroxides; C085707:poly(dimethylsiloxane)-polyamide copolymer