title: Respiratory syncytial virus subunit vaccine based on a recombinant fusion protein expressed transiently in mammalian cells.
abstract: Although respiratory syncytial virus (RSV) causes severe lower respiratory tract infection in infants and adults at risk, no RSV vaccine is currently available. In this report, efforts toward the generation of an RSV subunit vaccine using recombinant RSV fusion protein (rRSV-F) are described. The recombinant protein was produced by transient gene expression (TGE) in suspension-adapted human embryonic kidney cells (HEK-293E) in 4 L orbitally shaken bioreactors. It was then purified and formulated in immunostimulating reconstituted influenza virosomes (IRIVs). The candidate vaccine induced anti-RSV-F neutralizing antibodies in mice, and challenge studies in cotton rats are ongoing. If successful in preclinical and clinical trials, this will be the first recombinant subunit vaccine produced by large-scale TGE in mammalian cells.
authors: Ecole Polytechnique Fédérale de Lausanne (EPFL), Institute of Bioengineering, Laboratory of Cellular Biotechnology, CH-1015 Lausanne, Switzerland.
authors: Sophie
authors: S
authors: Nallet
authors: Mario
authors: M
authors: Amacker
authors: Nicole
authors: N
authors: Westerfeld
authors: Lucia
authors: L
authors: Baldi
authors: Iwo
authors: I
authors: König
authors: David L
authors: DL
authors: Hacker
authors: Christiane
authors: C
authors: Zaborosch
authors: Rinaldo
authors: R
authors: Zurbriggen
authors: Florian M
authors: FM
authors: Wurm
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D002460:Cell Line; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D009500:Neutralization Tests; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification; D018357:Respiratory Syncytial Virus Infections / Q000276:immunology / Q000517:prevention & control*; D022261:Respiratory Syncytial Virus Vaccines / Q000276:immunology*; D014162:Transfection; D022223:Vaccines, Subunit / Q000276:immunology; D014760:Viral Fusion Proteins / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification; D022701:Virosomes / Q000276:immunology
chemical_list: D000914:Antibodies, Viral; C526850:F protein, human respiratory syncytial virus; D011993:Recombinant Fusion Proteins; D022261:Respiratory Syncytial Virus Vaccines; D022223:Vaccines, Subunit; D014760:Viral Fusion Proteins; D022701:Virosomes