Highly sensitive mutation detection based on digital amplification coupled with hydrogel bead-array.
In order to detect small amounts of mutants for early cancer diagnosis, we have developed the novel method of using amplicon-coated microbeads and single-molecule-PCR in water-in-oil emulsions, which we coupled with a new detection platform, the hydrogel bead-array, a 3-D polyacrylamide gel network used as a carrier to immobilize the beads.
Huang
Huan
H
Huadong Research Institute for Medicine and Biotechnics, Nanjing 210002, China.
Qi
Zongtai
Z
Deng
Lili
L
Zhou
Guohua
G
Kajiyama
Tomoharu
T
Kambara
Hideki
H
D015179:Colorectal Neoplasms / Q000175:diagnosis / Q000235:genetics; D004247:DNA / Q000235:genetics*; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D004655:Emulsions; D006801:Humans; D020100:Hydrogels / Q000737:chemistry*; D008863:Microspheres; D009154:Mutation; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity
D004655:Emulsions; D020100:Hydrogels; D004247:DNA