title: MATER protein as substrate of PKCepsilon in human cumulus cells.
abstract: High activity of the phosphoinositide 3-kinase/Akt pathway in cumulus cells plays an important role in FSH regulation of cell function and Protein Kinase C epsilon (PKCepsilon) collaborates with these signalling pathways to regulate cell proliferation. Relevant roles in follicular development are played by Maternal Antigen That Embryos Require (MATER) that is a cumulus cell- and oocyte-specific protein dependent on the maternal genome. We recently demonstrated that human MATER localizes at specific domains of oocytes and, for the first time, also in cumulus cells. MATER contains a carboxy-terminal leucine-rich repeat domain involved in protein-protein interactions regulating different cellular functions. Here we investigated the functional role of MATER. Thus, we performed coimmunoprecipitation experiments using HEK293T cells expressing human MATER; a similar approach was then followed in human cumulus/follicular cells. In MATER(+)HEK293T cells, we observed that this protein acts as a phosphorylation substrate of PKCepsilon. Western blot experiments indicate that, unlike oocytes, human cumulus cells express PKCepsilon. Immunoprecipitation and confocal analysis suggest for the first time that MATER protein interacts with this protein kinase in cumulus cells under physiological conditions. Since PKCepsilon is known to collaborate with antiapoptotic signalling pathways, this suggests a novel mechanism for the function of MATER in follicular maturation.
authors: Department of Anatomy and Histology, University of Modena and Reggio Emilia, Via Del Pozzo 71, 41100 Modena, Italy. tullia.maraldi@unimo.it
authors: T
authors: T
authors: Maraldi
authors: M
authors: M
authors: Riccio
authors: P
authors: P
authors: Sena
authors: L
authors: L
authors: Marzona
authors: A
authors: A
authors: Nicoli
authors: A
authors: A
authors: La Marca
authors: S
authors: S
authors: Marmiroli
authors: J
authors: J
authors: Bertacchini
authors: G
authors: G
authors: La Sala
authors: A
authors: A
authors: De Pol
mesh_terms: D001324:Autoantigens / Q000235:genetics / Q000378:metabolism*; D002460:Cell Line; D002478:Cells, Cultured; D054885:Cumulus Cells / Q000378:metabolism*; D004586:Electrophoresis; D006801:Humans; D047468:Immunoprecipitation; D018613:Microscopy, Confocal; D008856:Microscopy, Fluorescence; D024101:Mitochondrial Proteins; D009687:Nuclear Proteins; D010766:Phosphorylation; D011485:Protein Binding; D051744:Protein Kinase C-epsilon / Q000378:metabolism*
chemical_list: D001324:Autoantigens; D024101:Mitochondrial Proteins; C527221:NLRP5 protein, human; D009687:Nuclear Proteins; D051744:Protein Kinase C-epsilon