The influence of powdered coconut water (ACP-318®) in in vitro maturation of canine oocytes.
The objective of this study was to determine the influence of powdered coconut water (ACP-318(®)) diluted in high glucose (11.0 mM) TCM199 in the achievement of nuclear in vitro maturation (IVM) of canine oocytes. Cumulus oocyte complexes (COCs) (n = 632) were randomly allocated into three experimental groups named as group 1 (control group), group 2 (5% powdered coconut water) and group 3 (10% powdered coconut water). The percentage of meiotic resumption (MR) (GVBD to MII) was 39.1% (81/207), 50.2% (108/215) and 46.6% (98/210) for groups 1, 2 and 3 respectively (p < 0.05). There were no differences in MR rates among groups 2 and 3. The medium with ACP-318(®) slightly enhanced the nuclear maturation of canine oocytes when a comparison was established with rates of maturation exhibited by oocytes in the experimental group 1 without ACP-318(®) (p < 0.05). The results suggest that oocytes' nuclear morphology integrity and meiosis achievement were positively influenced when exposed to high glucose TCM199 supplemented with 5% powdered coconut water. Further investigation must be performed for a better understanding of powdered coconut water influence in cellular events during IVM of dog oocytes.
Silva
A E F
AE
Laboratory of Embryology and Biotechnics of Reproduction, Faculty of Veterinary Medicine, UFRGS, Porto Alegre, RS, Brazil.
Cavalcante
L F
LF
Rodrigues
B A
BA
Rodrigues
J L
JL
D000818:Animals; D018929:Cell Culture Techniques; D003059:Cocos / Q000737:chemistry*; D003470:Culture Media / Q000737:chemistry*; D004285:Dogs; D005260:Female; D008540:Meiosis; D009865:Oocytes / Q000166:cytology* / Q000502:physiology*; D014867:Water / Q000737:chemistry*
D003470:Culture Media; D014867:Water