Cytoprotective role of astaxanthin against glycated protein/iron chelate-induced toxicity in human umbilical vein endothelial cells.
Astaxanthin (ASX), a red carotenoid pigment with no pro-vitamin A activity, is a biological antioxidant that occurs naturally in a wide variety of plants, algae and seafoods. This study investigated whether ASX could inhibit glycated protein/iron chelate-induced toxicity in human umbilical-vein endothelial cells (HUVEC) by interfering with ROS generation in these cells. Glycated fetal bovine serum (GFBS) was prepared by incubating fetal bovine serum (FBS) with high-concentration glucose. Stimulation of cultured HUVECs with 50 mm 1 mL of GFBS significantly enhanced lipid peroxidation and decreased antioxidant enzyme activities and levels of phase II enzymes. However, preincubation of the cultures with ASX resulted in a marked decrease in the level of lipid peroxide (LPO) and an increase in the levels of antioxidant enzymes in an ASX concentration-dependent manner. These results demonstrate that ASX could inhibit LPO formation and enhance the antioxidant enzyme status in GFBS/iron chelate-exposed endothelial cells by suppressing ROS generation, thereby limiting the effects of the AGE-RAGE interaction. The results indicate that ASX could have a beneficial role against glycated protein/iron chelate-induced toxicity by preventing lipid and protein oxidation and increasing the activity of antioxidant enzymes.
Nishigaki
Ikuo
I
NPO International Laboratory of Biochemistry, 1-166 Uchide, Nakagawa-ku Nagoya 454-0926, Japan. nishigaki@se.starcat.ne.jp
Rajendran
Peramaiyan
P
Venugopal
Ramachandran
R
Ekambaram
Gnapathy
G
Sakthisekaran
Dhanapal
D
Nishigaki
Yutaka
Y
D000975:Antioxidants / Q000378:metabolism*; D002460:Cell Line; D042783:Endothelial Cells / Q000187:drug effects*; D006801:Humans; D007502:Iron Chelating Agents; D015227:Lipid Peroxidation / Q000187:drug effects*; D008054:Lipid Peroxides / Q000378:metabolism; D018384:Oxidative Stress; D010088:Oxidoreductases / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D014471:Umbilical Veins / Q000166:cytology; D024341:Xanthophylls / Q000494:pharmacology
D000975:Antioxidants; D007502:Iron Chelating Agents; D008054:Lipid Peroxides; D017382:Reactive Oxygen Species; D024341:Xanthophylls; C005948:astaxanthine; D010088:Oxidoreductases