title: Early metabolic reactivation versus antioxidant therapy after a traumatic spinal cord injury in adult rats.
abstract: Disability after traumatic spinal cord injury (TSCI) results from physical trauma and from "secondary mechanisms of injury" such as low metabolic energy levels, oxidative damage and lipid peroxidation. In order to prove if early metabolic reactivation is a better therapeutic option than antioxidant therapy in the acute phase of TSCI, spinal cord contusions were performed in adult rats using a well-characterized weight drop technique at thoracic 9 level. After TSCI, pyrophosphate of thiamine or non-degradable cocarboxylase (NDC) enzyme was used to maintain energy levels, antioxidants such as superoxide dismutase and catalase (ANT) were used to decrease oxidative damage and methylprednisolone (MP), which has both therapeutic properties, was used as a control. Rats were divided into one sham group and six with TSCI; one of them received no treatment, and the rest were treated with NDC, MP, NDC + MP, NDC + ANT or ANT. The ANT group decreased lactate and creatine phosphokinase levels and increased the amount of preserved tissue (morphometric analysis) as well as functional recovery (Basso, Beattie and Bresnahan or BBB motor scale). In contrast, NDC treatment increased lipid peroxidation, measured through thiobarbituric acid reactive substances (TBARS) levels, as well as spinal cord tissue destruction and functional deficit. Early metabolic reactivation after a TSCI may be deleterious, while natural early metabolic inhibition may not be a "secondary mechanism of injury" but a "secondary neuroprotective response". While increased antioxidant defence after a TSCI may currently be an ideal therapeutic strategy, the usefulness of metabolic reactivation should be tested in the sub-acute or chronic phases of TSCI and new strategies must continue to be tested for the early ones.
authors: Research Medical Unit in Neurological Diseases, National Medical Center, XXI Century, IMSS, Mexico City, Mexico.
authors: Sergio
authors: S
authors: Torres
authors: Hermelinda
authors: H
authors: Salgado-Ceballos
authors: José Luis
authors: JL
authors: Torres
authors: Sandra
authors: S
authors: Orozco-Suarez
authors: Araceli
authors: A
authors: Díaz-Ruíz
authors: Angelina
authors: A
authors: Martínez
authors: Mario
authors: M
authors: Rivera-Cruz
authors: Camilo
authors: C
authors: Ríos
authors: Alicia
authors: A
authors: Lara
authors: Carlos
authors: C
authors: Collado
authors: Gabriel
authors: G
authors: Guizar-Sahagún
mesh_terms: D000208:Acute Disease; D000375:Aging; D000818:Animals; D000975:Antioxidants / Q000627:therapeutic use*; D002374:Catalase / Q000627:therapeutic use; D003402:Creatine Kinase / Q000378:metabolism; D005260:Female; D019344:Lactic Acid / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D011897:Random Allocation; D051381:Rats; D020318:Rats, Long-Evans; D020127:Recovery of Function; D013116:Spinal Cord / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D013119:Spinal Cord Injuries / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D013482:Superoxide Dismutase / Q000627:therapeutic use; D013835:Thiamine Pyrophosphate / Q000627:therapeutic use*; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism; D016896:Treatment Outcome; D014803:Vitamin B Complex / Q000627:therapeutic use*
chemical_list: D000975:Antioxidants; D017392:Thiobarbituric Acid Reactive Substances; D014803:Vitamin B Complex; D019344:Lactic Acid; D002374:Catalase; D013482:Superoxide Dismutase; D003402:Creatine Kinase; D013835:Thiamine Pyrophosphate