title: Determination of glutathione and glutathione disulfide in biological samples: an in-depth review.
abstract: Glutathione (GSH) is a thiol-containing tripeptide, which plays central roles in the defence against oxidative damage and in signaling pathways. Upon oxidation, GSH is transformed to glutathione disulfide (GSSG). The concentrations of GSH and GSSG and their molar ratio are indicators of cell functionality and oxidative stress. Assessment of redox homeostasis in various clinical states and medical applications for restoration of the glutathione status are of growing importance. This review is intended to provide a state-of-the-art overview of issues relating to sample pretreatment and choices for the separation and detection of GSH and GSSG. High-performance liquid chromatography, capillary electrophoresis and gas chromatography (as techniques with a separation step) with photometric, fluorimetric, electrochemical and mass spectrometric detection are discussed, stress being laid on novel approaches.
authors: Department of Pediatrics, University of Szeged, Szeged, Hungary.
authors: Péter
authors: P
authors: Monostori
authors: Gyula
authors: G
authors: Wittmann
authors: Eszter
authors: E
authors: Karg
authors: Sándor
authors: S
authors: Túri
mesh_terms: D000818:Animals; D002623:Chemistry Techniques, Analytical / Q000379:methods*; D005978:Glutathione / Q000032:analysis*; D019803:Glutathione Disulfide / Q000032:analysis*; D006801:Humans; D010084:Oxidation-Reduction
chemical_list: D005978:Glutathione; D019803:Glutathione Disulfide