[Study on the difference of genes and the type identification of hantavirus from Lishui, Zhejiang province].
OBJECTIVE
To isolate hantavirus from Lishui county--one of the epidemic regions for hemorrhagic fever with renal syndrome (HFRS), in Zhejiang province, and to identify the serotype and molecular/biological characteristics of a new HTN subtype hantavirus (HV) strains, hopefully to provide evidence for HFRS prevention and therapy.


METHODS
Data on the host animals was collected from Lishui, Zhejiang province in 2007. Direct immunofluorscece assay was adopted to determine HFRS antigens and the lung tissues from HV infected Vero-E6 cells for HV isolation, then total RNA was extracted from Hantavirus Lishui strains and amplified by RT-PCR M, S segments of strains genome were also cloned and sequenced and compared with those of other strains of HV.


RESULTS
2 strains virus (ZLS6-11 and ZLS-12) were successfully isolated from 7 positive lung samples of mice and were identified as HTNV by anti-McAb and phylogenetic analysis. With sequence compation,we found that 2 strains with complete M and S segment had higher homology with HTN-type strains than with other types of HV, but 13.4%-20.7% and 10.3%-16.1% of the genes were found which were different from HTNV. The phylogenetic trees constructed by complete S and M segment showed that ZLS6-11 and ZLS-12 strains were located in HTNV group,and structured independent embranchment.


CONCLUSIONS
ZLS6-11 and ZLS-12 Strains were believed to belong to HTN-type and phylogenetically different from the HTNV.
Yao
Ping-ping
PP
Zhejiang Center for Disease Control and Prevention, Hangzhou 310051, China.
Zhu
Han-ping
HP
Xu
Fang
F
Wang
Zhi-gang
ZG
Mei
Ling-ling
LL
Lei
Yong-liang
YL
Xie
Rong-hui
RH
Wang
Fu-su
FS
Zhu
Zhi-yong
ZY
Deng
Xiao-zhao
XZ
Zhang
Yun
Y
D000818:Animals; D000956:Antigens, Viral / Q000032:analysis; D002681:China / Q000453:epidemiology; D002522:Chlorocebus aethiops; D005838:Genotype; D006476:Orthohantavirus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D006480:Hemorrhagic Fever with Renal Syndrome / Q000453:epidemiology / Q000821:virology*; D051379:Mice; D051189:Murinae; D010802:Phylogeny; D012367:RNA, Viral / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017423:Sequence Analysis, RNA; D014709:Vero Cells
D000956:Antigens, Viral; D012367:RNA, Viral