Investigation of natural phosphatidylcholine sources: separation and identification by liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS2) of molecular species.
This study is a contribution to the exploration of natural phospholipid (PL) sources rich in long-chain polyunsaturated fatty acids (LC-PUFAs) with nutritional interest. Phosphatidylcholines (PCs) were purified from total lipid extracts of different food matrices, and their molecular species were separated and identified by liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS(2)). Fragmentation of lithiated adducts allowed for the identification of fatty acids linked to the glycerol backbone. Soy PC was particularly rich in species containing essential fatty acids, such as (18:2-18:2)PC (34.0%), (16:0-18:2)PC (20.8%), and (18:1-18:2)PC (16.3%). PC from animal sources (ox liver and egg yolk) contained major molecular species, such as (16:0-18:2)PC, (16:0-18:1)PC, (18:0-18:2)PC, or (18:0-18:1)PC. Finally, marine source (krill oil), which was particularly rich in (16:0-20:5)PC and (16:0-22:6)PC, appeared to be an interesting potential source for food supplementation with LC-PUFA-PLs, particularly eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA).
Le Grandois
Julie
J
Laboratoire de Chimie Analytique et Sciences de l'Aliment, IPHC-DSA, Universite de Strasbourg, CNRS, 67400 Illkirch, France.
Marchioni
Eric
E
Zhao
Minjie
M
Giuffrida
Francesca
F
Ennahar
Saïd
S
Bindler
Françoise
F
D000818:Animals; D002417:Cattle; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004281:Docosahexaenoic Acids / Q000032:analysis; D004530:Egg Yolk / Q000737:chemistry; D015118:Eicosapentaenoic Acid / Q000032:analysis; D033201:Euphausiacea / Q000737:chemistry; D005231:Fatty Acids, Unsaturated / Q000032:analysis; D005504:Food Analysis / Q000379:methods*; D008099:Liver / Q000737:chemistry; D009821:Oils / Q000737:chemistry; D010713:Phosphatidylcholines / Q000737:chemistry* / Q000302:isolation & purification; D013025:Glycine max / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
D005231:Fatty Acids, Unsaturated; D009821:Oils; D010713:Phosphatidylcholines; D004281:Docosahexaenoic Acids; D015118:Eicosapentaenoic Acid