Involvement of cellular proteins in Junin arenavirus entry.
Junin arenavirus (JUNV) entry is dependent on clathrin-mediated pathways and it relies on the integrity of the actin cytoskeleton as well as the dynamics of microtubules. To determine the method of entry used by this human pathogen, we have demonstrated that in Vero cells JUNV is trafficked via the cellular dynamin 2 (dyn2) endocytic pathway and it is dependent on the Eps15 GTPase. In addition, we have shown that the virus travels through Rab5-mediated early and Rab7-mediated late endosomes in its pH-dependent entry. Altogether, this study gives further inside into the endocytic pathway utilized by the arenavirus JUNV.
Martinez
M Guadalupe
MG
Laboratorio de Virología, Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina.
Forlenza
M Belen
MB
Candurra
Nélida A
NA
D000818:Animals; D002135:Calcium-Binding Proteins / Q000378:metabolism; D002522:Chlorocebus aethiops; D034285:Dynamin II / Q000378:metabolism; D004705:Endocytosis / Q000502:physiology; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins / Q000378:metabolism; D018051:Junin virus / Q000378:metabolism / Q000502:physiology*; D051379:Mice; D010750:Phosphoproteins / Q000378:metabolism; D014709:Vero Cells; D019562:Viral Load; D014764:Viral Proteins / Q000378:metabolism / Q000502:physiology*; D053586:Virus Internalization; D020691:rab GTP-Binding Proteins / Q000378:metabolism; D020696:rab5 GTP-Binding Proteins / Q000378:metabolism; D000090802:rab7 GTP-Binding Proteins
D002135:Calcium-Binding Proteins; D047908:Intracellular Signaling Peptides and Proteins; D010750:Phosphoproteins; D014764:Viral Proteins; D000090802:rab7 GTP-Binding Proteins; C000716463:rab7 GTP-binding proteins, human; C000716465:rab7 GTP-binding proteins, mouse; D020691:rab GTP-Binding Proteins; D020696:rab5 GTP-Binding Proteins; D034285:Dynamin II