[Initial RAPD analysis of Isatis indigotica somatic clones' resistance to root rot disease].
OBJECTIVE
To make initial identification on Isatis Indigotica Somatic Clones' resistant mutant.


METHODS
The resistant plants were selected under the treatment of root-rot disease crude toxin. The DNA of the same clones and their parents were analysed by RAPD technology.


RESULTS
We got initial resistance of the plants and selected 12 primers of which most were identical but the less were different. The genetic background of the somatic clones was identical but the individuals were mutated on the DNA level.


CONCLUSIONS
It provides experimental foundation for further study on separating resistant mutant and resistant gene.
Chen
Gui-Ping
GP
Department of Biology Science, Tangshan Teachers College, Tangshan 063000, China. chenguiping968@sohu.com
Ke
Shao-Ying
SY
Chen
Yu-Qin
YQ
D002999:Clone Cells; D017931:DNA Primers; D018744:DNA, Plant / Q000235:genetics / Q000302:isolation & purification; D031219:Isatis / Q000235:genetics* / Q000254:growth & development; D010935:Plant Diseases / Q000235:genetics* / Q000382:microbiology; D010946:Plants, Medicinal / Q000235:genetics* / Q000254:growth & development; D019105:Random Amplified Polymorphic DNA Technique; D012639:Seeds / Q000254:growth & development; D046509:Tissue Culture Techniques
D017931:DNA Primers; D018744:DNA, Plant