Intrabody expression in eukaryotic cells.
We describe procedures for intracellular expression of scFv in eukaryotic cells. Starting from a scFv gene cloned in a phage-display vector, we describe the cloning step into a mammalian expression vector, the transient transfection of a HeLa cell line, and the monitoring of intrabody expression by immunofluorescence staining and FACS analysis.
Guglielmi
Laurence
L
IRCM, Institut de Recherche en Cancérologie de Montpellier, Montpellier F-34298, France.
Martineau
Pierre
P
D005434:Flow Cytometry / Q000379:methods*; D005822:Genetic Vectors; D006367:HeLa Cells / Q000276:immunology; D006801:Humans; D007135:Immunoglobulin Variable Region / Q000276:immunology*; D008856:Microscopy, Fluorescence / Q000379:methods*
D007135:Immunoglobulin Variable Region