A phenotypic study of TFS1 mutants differentially altered in the inhibition of Ira2p or CPY.
The Saccharomyces cerevisiae protein Tfs1p is known as a dual protein. On the one hand, it inhibits the carboxypeptidase Y protease, and on the other, it inhibits Ira2p, a GTPase-activating protein of Ras. We managed to dissect precise areas of Tfs1p specifically involved in only one of those functions. Based on these data, specific Tfs1p point mutants affected in only one of these two functions were constructed. In order to obtain insights on the physiological role of these functions, systematic phenotypic tests were performed on strains expressing these specific Tfs1p mutants. The results obtained demonstrate that the inhibition of Ira2p by Tfs1p is the predominant function under the conditions tested.
Gombault
Aurélie
A
Centre de Biophysique Moléculaire, Centre National de la Recherche Scientifique, UPR 4301, Affiliated to the University of Orléans and INSERM, Orléans, France.
Warringer
Jonas
J
Caesar
Robert
R
Godin
Fabienne
F
Vallée
Béatrice
B
Doudeau
Michel
M
Chautard
Hélène
H
Blomberg
Anders
A
Bénédetti
Hélène
H
D019943:Amino Acid Substitution; D002268:Carboxypeptidases / Q000037:antagonists & inhibitors*; D002352:Carrier Proteins / Q000235:genetics / Q000502:physiology*; D020690:GTPase-Activating Proteins / Q000037:antagonists & inhibitors*; D015966:Gene Expression Regulation, Fungal; D047908:Intracellular Signaling Peptides and Proteins; D050505:Mutant Proteins / Q000235:genetics / Q000502:physiology; D020125:Mutation, Missense; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000502:physiology*; D029701:Saccharomyces cerevisiae Proteins / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000502:physiology*
D002352:Carrier Proteins; D020690:GTPase-Activating Proteins; C064960:IRA2 protein, S cerevisiae; D047908:Intracellular Signaling Peptides and Proteins; D050505:Mutant Proteins; D029701:Saccharomyces cerevisiae Proteins; C492432:Tfs1 protein, S cerevisiae; D002268:Carboxypeptidases; C520242:CPY protein, S cerevisiae