title: Flavonoid characterization and antioxidant activity of hydroalcoholic extracts from Achillea ligustica All.
abstract: Ethanolic extracts of Achillea ligustica All. (Asteraceae) flowering tops were evaluated. High-performance liquid chromatography-electrospray ionisation-mass spectrometry was used for the identification and quantification of phenolic compounds. 6-Hydroxykaempferol-3,6,4'-trimethyl ether, apigenin-6-C-glucoside-8-C-arabinoside, luteolin, and apigenin were the most abundant flavonoids. For the first time C-glycosylflavones were detected in A. ligustica with apigenin-6-C-glucoside-8-C-arabinoside being the most representative. The radical scavenging activity of the extracts was determined by DPPH test and ranged between 4.18 and 12.3 mM. The ability of these extracts to inhibit non-enzymatic lipid peroxidation was studied using the simple in vitro system of linoleic acid oxidation: five of the nine extracts exerted a protective effect at the lower amount tested (5 microg). Protection on CaCo-2 intestinal cells against TBH-induced toxicity was also investigated: the results showed that two of the extracts tested in this cell system had the ability to protect against oxidative stress induced by TBH starting from concentrations as low as 10 microg/ml.
authors: Dipartimento di Tossicologia, University of Cagliari, via Ospedale 72, 09124 Cagliari, Italy. tuberoso@unica.it
authors: Carlo Ignazio Giovanni
authors: CI
authors: Tuberoso
authors: Paola
authors: P
authors: Montoro
authors: Sonia
authors: S
authors: Piacente
authors: Giulia
authors: G
authors: Corona
authors: Monica
authors: M
authors: Deiana
authors: Maria Assunta
authors: MA
authors: Dessì
authors: Cosimo
authors: C
authors: Pizza
authors: Paolo
authors: P
authors: Cabras
mesh_terms: D031162:Achillea / Q000737:chemistry*; D000975:Antioxidants / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology*; D018938:Caco-2 Cells; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004305:Dose-Response Relationship, Drug; D005419:Flavonoids / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology*; D016166:Free Radical Scavengers / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology; D006801:Humans; D018384:Oxidative Stress / Q000187:drug effects; D010636:Phenols / Q000302:isolation & purification / Q000494:pharmacology; D010936:Plant Extracts / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods
chemical_list: D000975:Antioxidants; D005419:Flavonoids; D016166:Free Radical Scavengers; D010636:Phenols; D010936:Plant Extracts