[Effects of enrofloxacin on DNA sequence diversity of soil cultural bacterial community].
In order to understand the effects of remained enrofloxacin (ENR) in environment on the diversity of soil microbial communities, amplified ribosomal DNA restriction analysis (ARDRA) approach and genomic fingerprinting technique enterobacterial repetitive intergenic consensus sequence-polymerase chain reaction (ERIC-PCR) were used to analyze the molecular diversity of 16S rDNA from soil bacteria after ENR addition. The results showed that after the ENR addition for 35 days, the total count of soil bacteria was less than that of CK, and decreased with increasing ENR concentration. The ARDRA divided the separated soil bacteria into different operational taxonomic units (OTU) groups, and the count of group I to group VI was 15, 13, 10, 8, 6, and 6, respectively. Genomic fingerprinting analysis indicated that the Shannon-Wiener index of group I to group VI was 2.78, 2.14, 1.78, 1.11, 0.69 and 0.31, respectively, and the Margalef index, Simpson index, and Pielou index of soil microbial community in CK were higher than that in the soils in which ENR was added.
Ma
Yi
Y
Guangdong Key Laboratory for Veterinary Pharmaceutics Development and Safety Evaluation, South China Agricultural University, Guangzhou 510642, China. mayi761@163.com
Chen
Zhang-Liu
ZL
D001419:Bacteria / Q000145:classification / Q000235:genetics*; D044822:Biodiversity; D004269:DNA, Bacterial / Q000032:analysis; D004275:DNA, Ribosomal / Q000032:analysis; D000077422:Enrofloxacin; D024841:Fluoroquinolones / Q000494:pharmacology*; D019105:Random Amplified Polymorphic DNA Technique; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000494:pharmacology*
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D024841:Fluoroquinolones; D012989:Soil Pollutants; D000077422:Enrofloxacin