Seroprevalence of Toxoplasma gondii in wild kangaroos using an ELISA.
Infection with Toxoplasma gondii is a significant problem in Australian marsupials, and can lead to devastating disease and predispose animals to predation. T. gondii infection in kangaroos is also of public health significance due to the kangaroo meat trade. A moderate seroprevalence of T. gondii was observed in a study of western grey kangaroos located in the Perth metropolitan area in Western Australia. Of 219 kangaroos tested, 15.5% (95%CI: 10.7-20.3) were positive for T. gondii antibodies using an ELISA developed to detect T. gondii IgG in macropod marsupials. When compared with the commercially available MAT (modified agglutination test), the ELISA developed was in absolute agreement and yielded a kappa coefficient of 1.00. Of 18 kangaroos tested for the presence of T. gondii DNA by PCR, the 9 ELISA positive kangaroos tested PCR positive and the 9 ELISA negative kangaroos tested PCR negative indicating the ELISA protocol was both highly specific and sensitive and correlated 100% with the more labour intensive PCR assay.
Parameswaran
N
N
WHO Collaborating Centre for the Molecular Epidemiology of Parasitic Infections, School of Veterinary and Biomedical Sciences, Murdoch University, Murdoch, WA 6150, Australia. nevi.parameswaran@gmail.com
O'Handley
R M
RM
Grigg
M E
ME
Fenwick
S G
SG
Thompson
R C A
RC
D000372:Agglutination Tests; D000818:Animals; D000835:Animals, Wild / Q000469:parasitology; D000913:Antibodies, Protozoan / Q000097:blood*; D001315:Australia / Q000453:epidemiology; D016054:DNA, Protozoan / Q000032:analysis / Q000302:isolation & purification; D004797:Enzyme-Linked Immunosorbent Assay; D007614:Macropodidae / Q000469:parasitology*; D016133:Polymerase Chain Reaction / Q000379:methods; D012680:Sensitivity and Specificity; D016036:Seroepidemiologic Studies; D014122:Toxoplasma / Q000235:genetics / Q000276:immunology*; D014124:Toxoplasmosis, Animal / Q000453:epidemiology* / Q000276:immunology / Q000469:parasitology
D000913:Antibodies, Protozoan; D016054:DNA, Protozoan