Use of anionic polymer, poly(methyl vinyl ether-maleic anhydride)-coated beads for capture of respiratory syncytial virus.
Respiratory syncytial virus (RSV) is the single most important cause of severe lower respiratory tract infections in infants and young children, and a major public health concern in pediatrics. However, current diagnostic methods for RSV are not sufficiently sensitive. In addition, there is no simple method for enhancing RSV detection. Here, a method for capturing RSV from nasal fluid has been developed using magnetic beads coated with an anionic polymer, poly(methyl vinyl ether-maleic anhydrate). The beads were incubated with RSV-infected nasal fluid, then separated from the supernatant by applying a magnet field and washed. The adsorption [corrected] of RSV by the beads was confirmed by immunochromatography, reverse transcription-polymerase chain reaction, Western blotting and an enzyme-linked immunosorbent assay, which indicated the presence of nucleocapsid protein, fusion protein, and the viral genome of RSV on the incubated beads. Therefore, this capture method will contribute to the improvement of RSV detection.
Sakudo
Akikazu
A
Department of Virology, Center for Infectious Disease Control, Research Institute for Microbial Diseases, Osaka University, Yamadaoka, Suita, Osaka 565-0871, Japan. sakudo@biken.osaka-u.ac.jp
Baba
Koichi
K
Tsukamoto
Megumi
M
Ikuta
Kazuyoshi
K
D000838:Anions; D000998:Antiviral Agents / Q000138:chemical synthesis* / Q000494:pharmacology; D015153:Blotting, Western; D002626:Chemistry, Pharmaceutical / Q000379:methods; D002845:Chromatography / Q000379:methods; D015195:Drug Design; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D006801:Humans; D008298:Maleates / Q000138:chemical synthesis* / Q000494:pharmacology; D019590:Nucleocapsid Proteins / Q000737:chemistry; D011095:Polyethylenes / Q000138:chemical synthesis* / Q000494:pharmacology; D011108:Polymers / Q000737:chemistry*; D018357:Respiratory Syncytial Virus Infections / Q000188:drug therapy / Q000378:metabolism; D012136:Respiratory Syncytial Viruses / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction
D000838:Anions; D000998:Antiviral Agents; D008298:Maleates; D019590:Nucleocapsid Proteins; D011095:Polyethylenes; D011108:Polymers; C043105:poly(methyl vinyl ether-co-maleic anhydride)