Characterization of short-lived intermediates produced during replication of baculovirus DNA.
In this report the short-lived DNA replication intermediates produced in both uninfected and Autographa californica multiple nucleopolyhedrovirus (AcMNPV) infected Spodoptera frugiperda cells were characterized. The methods used included pulse-labeling of DNA in permiabilized cells, treatment of nascent DNA with Mung bean nuclease, and electrophoresis in neutral and alkaline agarose gels. In contrast to uninfected cells that produced a population of small DNA fragments of about 200bp, a population of heterogeneous fragments of up to 5kb with an average size of 1-2kb derived randomly from the virus genome was identified as the short-lived intermediates produced during AcMNPV replication. The intermediates likely include Okazaki fragments derived from the lagging strands in viral replication forks as well as fragments produced during the recombination-dependent replication.
Mikhailov
Victor S
VS
N. K. Koltzov Institute of Developmental Biology, Russian Academy of Sciences, Moscow, Russia. mikhailov48@mail.ru
Rohrmann
George F
GF
D000818:Animals; D002460:Cell Line; D004247:DNA / Q000096:biosynthesis*; D004261:DNA Replication; D004279:DNA, Viral / Q000378:metabolism; D017924:Nucleopolyhedroviruses / Q000502:physiology*; D010940:Plant Proteins / Q000378:metabolism; D015719:Single-Strand Specific DNA and RNA Endonucleases / Q000378:metabolism; D018411:Spodoptera / Q000235:genetics / Q000821:virology*; D014779:Virus Replication
D004279:DNA, Viral; C526101:Mung Bean Nuclease; C022262:Okazaki fragments; D010940:Plant Proteins; D004247:DNA; D015719:Single-Strand Specific DNA and RNA Endonucleases