title: Alcohol dependence-related increase of glial cell density in the anterior cingulate cortex of suicide completers.
abstract: BACKGROUND
  Suicide is the most serious consequence of major depressive disorder (MDD). Although the anterior cingulate cortex (ACC; Brodmann area [BA] 24) has been increasingly investigated for its role in the etiology of MDD, there is surprisingly very little information about the possible implication of this brain region in suicide. We hypothesized that changes in BA24 cell densities occur in depressed individuals who commit suicide, possibly reflecting an altered state of cortical plasticity that is thought to occur in depression.
  
  
  METHODS
  We investigated cell densities and sizes in BA24 among suicide completers and matched sudden-death controls. We examined a 1-cm(3) tissue block from BA24a of the supracallosal ACC in 26 human postmortem brain specimens (13 depressed individuals who committed suicide and 13 controls). We assessed neuronal and glial cell densities as well as neuronal soma sizes in the upper and lower cortical layers using optical fractionator and nucleator 3-dimensional stereological probes.
  
  
  RESULTS
  Glial densities, neuronal densities and soma sizes measured in BA24a did not differ significantly between controls and suicide completers. Secondary analyses showed a significant and robust increase in glial cell densities in BA24a of alcohol-dependent depressed suicide completers compared with depressed suicide completers who were not alcohol-dependent (38%) and, to a lesser extent, controls (30%).
  
  
  CONCLUSIONS
  Our study, conducted with tissue samples from men only, made use of a nonspecific stain that does not distinguish between neuronal or glial cell subtypes. Furthermore, the quantitative analysis concerned upper and lower cortical contours rather than individual cortical layers.
  
  
  CONCLUSIONS
  Our results indicate that in BA24, glial density, neuronal density and soma size are not affected in MDD and suicide. They also suggest that alcohol dependence has an important influence on glial densities in this key limbic structure.
authors: Departments of Psychiatry & Neurology and Neurosurgery, McGill University, Douglas Mental Health University Institute, Verdun, Canada.
authors: Christa
authors: C
authors: Hercher
authors: Martin
authors: M
authors: Parent
authors: Cecilia
authors: C
authors: Flores
authors: Lilian
authors: L
authors: Canetti
authors: Gustavo
authors: G
authors: Turecki
authors: Naguib
authors: N
authors: Mechawar
mesh_terms: D000293:Adolescent; D000328:Adult; D000437:Alcoholism / Q000150:complications / Q000473:pathology*; D016022:Case-Control Studies; D002452:Cell Count; D048429:Cell Size; D003865:Major Depressive Disorder / Q000150:complications / Q000473:pathology; D017831:Diagnosis, Dual (Psychiatry); D006179:Gyrus Cinguli / Q000473:pathology*; D006801:Humans; D008297:Male; D008875:Middle Aged; D009457:Neuroglia / Q000473:pathology*; D009474:Neurons / Q000473:pathology; D013405:Suicide