Stochastic transfer function for structured illumination microscopy.
The stochastic transfer function extends the concept of the conventional transfer function by incorporating noise statistics, thus giving a measure of the signal-to-noise ratio at each spatial frequency. This provides a convenient and standardized metric to assess the trade-off in terms of spatial frequency bandwidth and signal-to-noise ratio for a diverse range of resolution enhancement techniques. We apply this concept to structured illumination microscopy and compare its noise performance as a function of frequency with the conventional wide-field fluorescent microscope. The result suggests how a hybrid algorithm further improves the photon efficiency of the structured illumination technique.
Somekh
Michael G
MG
Institute of Biophysics Imaging and Optical Science, The University of Nottingham, University Park, Nottingham, UK. mike.somekh@nottingham.ac.uk
Hsu
Ken
K
Pitter
Mark C
MC
D008027:Light; D008853:Microscopy / Q000379:methods*; D008856:Microscopy, Fluorescence; D009010:Monte Carlo Method; D013269:Stochastic Processes