[Role of B7-H1 in pancreatic carcinoma immune evasion].
OBJECTIVE
To investigate the role of B7-H1 expression in IL-10 production, the B7-H1 and IL-10 expression levels in pancreatic carcinoma tissues and to analyze the correlation between B7-H1 expression and IL-10 level.


METHODS
The mRNA and protein levels expressions of B7-H1 and IL-10 in 35 cases of pancreatic cancer and corresponding paracarcinoma tissues and 5 cases of normal pancreas tissues were detected by RT-PCR, Western blot and immunohistochemistry respectively.


RESULTS
The findings for the first time provided the evidences that there was a clear trend for B7-H1 and IL-10 expressions to be most highly expressed in carcinoma tissue, intermediately expressed in paracarcinoma tissue, and expressed at the lowest level in normal pancreatic tissue at mRNA and protein levels. Moreover, there were statistically significant differences in B7-H1 and IL-10 expression between pancreatic carcinoma tissues, corresponding paracarcinoma tissues and normal pancreatic tissues at mRNA and protein levels (P < 0.05). Furthermore, the immunohistochemistry indicated that there were high expression levels of B7-H1 (60.5% +/- 12.7%) and IL-10 (65.3% +/- 16.2%) in pancreatic carcinoma tissues while there were no significant expressions in normal pancreatic tissues. Meanwhile, correlation analysis revealed that B7-H1 expression was significant associated with IL-10 level in tumor tissues at mRNA (P = 0.008, r = 0.841) and protein levels (P = 0.007, r = 0.838).


CONCLUSIONS
Over-expression of B7-H1 may be responsible for the increasing IL-10 production in pancreatic cancer, which caused reduced immune response to tumor cells and contributed to pancreatic carcinoma escape from immune attack.
Huang
Dong-Sheng
DS
Key Laboratory of Biotherapy of Zhejiang Province, Department of General Surgery, Sir Run Run Shaw Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310016, China. dshuang@zju.edu.cn
Liu
Jun-Wei
JW
Geng
Lei
L
Jiang
Guo-Ping
GP
Shen
Guo-Liang
GL
Yao
Wei-Feng
WF
D015703:Antigens, CD / Q000276:immunology*; D060890:B7-H1 Antigen; D006801:Humans; D057131:Immune Evasion; D016753:Interleukin-10 / Q000276:immunology*; D010190:Pancreatic Neoplasms / Q000276:immunology*
D015703:Antigens, CD; D060890:B7-H1 Antigen; C423236:CD274 protein, human; C508609:IL10 protein, human; D016753:Interleukin-10