Adenosine modulates alpha2-adrenergic receptors through a phospholipase C pathway in brainstem cell culture of rats.
Adenosine acts in the nucleus tractus solitarii (NTS), one of the main brain sites related to cardiovascular control. In the present study we show that A(1) adenosine receptor (A(1R)) activation promotes an increase on alpha(2)-adrenoceptor (Alpha(2R)) binding in brainstem cell culture from newborn rats. We investigated the intracellular cascade involved in such modulatory process using different intracellular signaling molecule inhibitors as well as calcium chelators. Phospholipase C, protein kinase Ca(2+)-dependent, IP(3) receptor and intracellular calcium were shown to participate in A(1R)/Alpha(2R) interaction. In conclusion, this result might be important to understand the role of adenosine within the NTS regarding autonomic cardiovascular control.
Carrettiero
Daniel Carneiro
DC
Natural and Human Science Center, Federal University of ABC-UFABC, Santo André, SP, Brazil.
da Silva
Sergio Marinho
SM
Fior-Chadi
Débora Rejane
DR
D000241:Adenosine / Q000378:metabolism* / Q000494:pharmacology; D000818:Animals; D017776:Autonomic Pathways / Q000187:drug effects / Q000378:metabolism; D001933:Brain Stem / Q000187:drug effects / Q000378:metabolism*; D002118:Calcium / Q000378:metabolism; D020013:Calcium Signaling / Q000187:drug effects / Q000502:physiology; D002320:Cardiovascular Physiological Phenomena / Q000187:drug effects; D002478:Cells, Cultured; D002614:Chelating Agents / Q000494:pharmacology; D006321:Heart / Q000294:innervation / Q000502:physiology; D053496:Inositol 1,4,5-Trisphosphate Receptors / Q000187:drug effects / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D018341:Receptors, Adrenergic, alpha-2 / Q000187:drug effects / Q000378:metabolism*; D015398:Signal Transduction / Q000187:drug effects / Q000502:physiology*; D017552:Solitary Nucleus / Q000187:drug effects / Q000378:metabolism*; D010738:Type C Phospholipases / Q000187:drug effects / Q000378:metabolism*
D002614:Chelating Agents; D053496:Inositol 1,4,5-Trisphosphate Receptors; D018341:Receptors, Adrenergic, alpha-2; D010738:Type C Phospholipases; D000241:Adenosine; D002118:Calcium