Lipopolysaccharide induced upregulation of beta-1,4-galactosyltransferase-I in Schwann cell.
beta4 Galactosylation of glycoproteins is one of the most important post-translational modifications. Recent studies have demonstrated that aberrant galactosylation associates with some inflammation diseases. beta-1,4-galactosyltransferase-I (beta-1,4-GalT-I), which transfers galactose to the terminal N-acetylglucosamine of N- and O-linked glycans in a beta-1,4- linkage, considered to be the major galactosyltransferse among the seven members of the subfamily responsible for beta4 galactosylation. In the present study, we investigated the expression of beta-1,4-GalT-I in Schwann cells under Lipopolysaccharide (LPS) treatment. RT-PCR revealed that the beta-1,4-GalT-I mRNA was significant increased as early as 2 h after LPS stimulation. Immunofluorescence showed that beta-1,4-GalT-I was located in Golgi apparatus and membrane of Schwann cells. With the 1 microg/ml LPS treatment, expression levels of beta-1,4-GalT-I was much higher compared with control group. In addition, lectin blot indicated that the beta4 galactosylation of glycoproteins such as integrin alpha5 was enhanced, which may due to the induced beta-1,4-GalT-I expression. These results suggested that beta-1,4-GalT-I may play an important role in adhesion and migration of Schwann cells during inflammation.
Yang
Huiguang
H
Department of Orthopaedics, Affiliated Jiangyin Renmin Hospital of Nantong University, Key Laboratory for Neuroregeneration of JiangSu Province, Nantong University, Jiangsu, People's Republic of China.
Hu
Ling
L
Chen
Jianping
J
Zhu
Jianchun
J
Tao
Tao
T
Zhang
Fupeng
F
Li
Xiaohong
X
He
Xingxin
X
Shen
Aiguo
A
Cheng
Chun
C
D000818:Animals; D002460:Cell Line; D016650:Fluorescein-5-isothiocyanate / Q000378:metabolism; D019085:Fluorescent Antibody Technique, Direct; D005456:Fluorescent Dyes / Q000378:metabolism; D005700:Galactosyltransferases / Q000235:genetics* / Q000378:metabolism*; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D007249:Inflammation / Q000139:chemically induced / Q000201:enzymology / Q000473:pathology; D008070:Lipopolysaccharides / Q000378:metabolism / Q000494:pharmacology*; D009237:N-Acetyllactosamine Synthase / Q000235:genetics* / Q000378:metabolism*; D051381:Rats; D012583:Schwann Cells / Q000378:metabolism*; D015854:Up-Regulation / Q000187:drug effects*
D005456:Fluorescent Dyes; D008070:Lipopolysaccharides; D005700:Galactosyltransferases; D009237:N-Acetyllactosamine Synthase; D016650:Fluorescein-5-isothiocyanate