[Investigation on induced expansion of erythroid cells from cord blood CD34(+) cells in vitro].
This study was aimed to investigate a beneficial approach for resolving the deficiency of blood source, preventing the infection resulting from blood transfusion and overcoming the knotty match of patients with rare blood group by using massive expansion of erythroid cells from cord blood CD34(+) cells in vitro. The CD34(+) cells from human cord blood were cultured in serum-free medium supplemented with stem cell factor (SCF), interleukin-3 (IL-3) and erythropoietin (EPO) for 1 week, then expansion and differentiation of CD34(+) cells into erythroid cells were supported by co-culture with human mesenchymal stem cells (MSCs) derived from bone marrow for 2 weeks. The results indicated that after culture for 23 days, the expansion multiple of total cell number reached 2.52 x 10(5), and over 95% of these cells were erythroid cells as compared with less than 1% of myelomonocytic (CD14(+) or CD15(+)) cells and megakaryocytic (CD41(+)) cells. However, the culture system without MSC support was significantly disadvantaged both in expansion ability and ratio of erythroid cells when compared with MSC supporting system. It is concluded that the erythroid cells can be produced from CD34(+) cells in large scale by culturing in the system comprised of cytokine sets and MSC feeders, in which MSCs can support the proliferation and differentiation of erythroid cells.
Jia
Yan-Jun
YJ
Beijing Red Cross Blood Center, Beijing, China.
Liu
Jiang
J
Zhang
Ke-Ying
KY
Shan
Xiao-Yan
XY
Li
Wei
W
He
Xiao-Mei
XM
Wang
Li-Jun
LJ
Liu
Na
N
Wang
Lin
L
Cui
Shuang
S
Ni
Lei
L
Zhao
Bo-Tao
BT
Gong
Zhi-Yin
ZY
Wang
Dong-Mei
DM
Gao
Song-Ming
SM
Zhang
Zhi-Xin
ZX
D018952:Antigens, CD34; D018929:Cell Culture Techniques / Q000379:methods*; D002454:Cell Differentiation; D005312:Fetal Blood / Q000166:cytology*; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D006801:Humans
D018952:Antigens, CD34