title: Immunogenicity and protective efficacy of Semliki forest virus replicon-based DNA vaccines encoding goatpox virus structural proteins.
abstract: Goatpox, caused by goatpox virus (GTPV), is an acute feverish and contagious disease in goats often associated with high morbidity and high mortality. To resolve potential safety risks and vaccination side effects of existing live attenuated goatpox vaccine (AV41), two Semliki forest virus (SFV) replicon-based bicistronic expression DNA vaccines (pCSm-AAL and pCSm-BAA) which encode GTPV structural proteins corresponding to the Vaccinia virus proteins A27, L1, A33, and B5, respectively, were constructed. Then, theirs ability to induce humoral and cellular response in mice and goats, and protect goats against virulent virus challenge were evaluated. The results showed that, vaccination with pCSm-AAL and pCSm-BAA in combination could elicit strong humoral and cellular responses in mice and goats, provide partial protection against viral challenge in goats, and reduce disease symptoms. Additionally, priming vaccination with the above-mentioned DNA vaccines could significantly reduce the goats' side reactions from boosting vaccinations with current live vaccine (AV41), which include skin lesions at the inoculation site and fevers. Data obtained in this study could not only facilitate improvement of the current goatpox vaccination strategy, but also provide valuable guidance to suitable candidates for evaluation and development of orthopoxvirus vaccines.
authors: Genetic Engineering Laboratory of PLA, Academy of Military Medical Sciences of PLA, Changchun 130062, PR China.
authors: Min
authors: M
authors: Zheng
authors: Ningyi
authors: N
authors: Jin
authors: Qi
authors: Q
authors: Liu
authors: Xiaowei
authors: X
authors: Huo
authors: Yang
authors: Y
authors: Li
authors: Bo
authors: B
authors: Hu
authors: Haili
authors: H
authors: Ma
authors: Zhanbo
authors: Z
authors: Zhu
authors: Yanzhao
authors: Y
authors: Cong
authors: Xiao
authors: X
authors: Li
authors: Minglan
authors: M
authors: Jin
authors: Guangze
authors: G
authors: Zhu
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood / Q000276:immunology; D000917:Antibody Formation; D018151:Capripoxvirus / Q000235:genetics / Q000276:immunology*; D049109:Cell Proliferation; D006041:Goats / Q000276:immunology* / Q000821:virology; D007111:Immunity, Cellular; D051379:Mice; D008807:Mice, Inbred BALB C; D009500:Neutralization Tests; D011213:Poxviridae Infections / Q000276:immunology; D012672:Semliki forest virus / Q000235:genetics / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology; D014611:Vaccination; D019444:Vaccines, DNA / Q000276:immunology*; D015678:Viral Structural Proteins / Q000235:genetics / Q000276:immunology*; D014765:Viral Vaccines / Q000276:immunology*
chemical_list: D000914:Antibodies, Viral; D019444:Vaccines, DNA; D015678:Viral Structural Proteins; D014765:Viral Vaccines