Characterizing and tracking single colloidal particles with video holographic microscopy.
We use digital holographic microscopy and Mie scattering theory to simultaneously characterize and track individual colloidal particles. Each holographic snapshot provides enough information to measure a colloidal sphere's radius and refractive index to within 1%, and simultaneously to measure its three-dimensional position with nanometer in-plane precision and 10 nanometer axial resolution.
Lee
Sang-Hyuk
SH
Department of Physics and Center for Soft Matter Research, New York University, New York, NY 10003, USA.
Roichman
Yohai
Y
Yi
Gi-Ra
GR
Kim
Shin-Hyun
SH
Yang
Seung-Man
SM
van Blaaderen
Alfons
A
van Oostrum
Peter
P
Grier
David G
DG
D000465:Algorithms; D003102:Colloids / Q000032:analysis* / Q000737:chemistry*; D006696:Holography / Q000379:methods*; D021621:Imaging, Three-Dimensional / Q000379:methods*; D044085:Microfluidics / Q000379:methods*; D018715:Microscopy, Video / Q000379:methods*; D010316:Particle Size; D012031:Refractometry / Q000379:methods*
D003102:Colloids