Ferromagnetic levan composite: an affinity matrix to purify lectin.
A simple and inexpensive procedure used magnetite and levan to synthesize a composite recovered by a magnetic field. Lectins from Canavalia ensiformis (Con A) and Cratylia mollis (Cramoll 1 and Cramoll 1, 4) did bind specifically to composite. The magnetic property of derivative favored washing out contaminating proteins and recovery of pure lectins with glucose elution. Cramoll 1 was purified by this affinity binding procedure in two steps instead of a previous three-step protocol with ammonium sulfate fractionation, affinity chromatography on Sephadex G-75, and ion exchange chromatography through a CM-cellulose column.
Angeli
Renata
R
Laboratório de Glicoproteínas, Departamento de Bioquímica, Universidade Federal de Pernambuco, 50670-901 Recife, PE, Brazil.
da Paz
Nathalia V N
NV
Maciel
Jackeline C
JC
Araújo
Flávia F B
FF
Paiva
Patrícia M G
PM
Calazans
Glícia M T
GM
Valente
Ana Paula
AP
Almeida
Fábio C L
FC
Coelho
Luana C B B
LC
Carvalho
Luiz B
LB
Silva
Maria da Paz C
Mda P
dos Santos Correia
Maria Tereza
MT
D032449:Canavalia / Q000737:chemistry; D002853:Chromatography, Liquid; D003208:Concanavalin A / Q000302:isolation & purification; D007887:Fabaceae / Q000737:chemistry; D052203:Ferrosoferric Oxide / Q000737:chemistry*; D005630:Fructans / Q000737:chemistry* / Q000378:metabolism; D006385:Hemagglutination Inhibition Tests; D019906:Nuclear Magnetic Resonance, Biomolecular; D037121:Plant Lectins / Q000302:isolation & purification* / Q000378:metabolism; D017355:Zymomonas / Q000737:chemistry
D005630:Fructans; D037121:Plant Lectins; D003208:Concanavalin A; C072599:levan; D052203:Ferrosoferric Oxide