Detection of quantum-dot labelled proteins using soft glass microstructured optical fibers.
The detection of quantum-dot labeled proteins is demonstrated within lead silicate soft glass microstructured optical fibers using near infrared light. The protein concentration is measured using a new fluorescence capture approach. Light guided within the fiber is used both to excite and collect fluorescent photons, and the detection limit achieved without optimization of the fiber geometry is 1 nM, using just 3% of the guided mode of the fiber. Issues that currently restrict the detection of lower protein concentrations are discussed.
Ruan
Yinlan
Y
Centre of Expertise in Photonics, School of Chemistry & Physics, University of Adelaide, Adelaide 5005, Australia. yinlan.ruan@adelaide.edu
Schartner
Erik P
EP
Ebendorff-Heidepriem
Heike
H
Hoffmann
Peter
P
Monro
Tanya M
TM
D017076:Computer-Aided Design; D055119:Elastic Modulus; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005336:Fiber Optic Technology / Q000295:instrumentation*; D005898:Glass / Q000737:chemistry; D008163:Luminescent Measurements / Q000295:instrumentation*; D011506:Proteins / Q000032:analysis*; D045663:Quantum Dots; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence / Q000706:statistics & numerical data*
D011506:Proteins