Analysis of antigenic cross-reactivity between subgroup C avian pneumovirus and human metapneumovirus by using recombinant fusion proteins.
Avian pneumovirus subgroup C (APV/C) has recently been reported to be more closely related to human metapneumovirus (hMPV) as determined by sequence analysis. To examine the antigenic relationship between APV/C and hMPV, the APV/C fusion (F) gene was cloned and expressed as an uncleaved glycoprotein in a baculovirus system. The reactivity of the APV/C F protein with antibodies against APV subgroups A, B, C, and hMPV was examined by Western blot analysis. The results showed that the expressed APV/C F protein was not only recognized by APV/C-specific antibodies but also by antibodies raised against hMPV. Previously expressed recombinant hMPV F protein also reacted with APV/C-specific antibodies, suggesting that there was significant antigenic cross-reactivity and a potential evolutionary relationship between hMPV and APV/C. Interestingly, the recombinant F proteins from APV/C and hMPV were not recognized by polyclonal antibodies specific to APV subgroups A and B.
Luo
L
L
National Centre for Foreign Animal Disease, Manitoba, Canada. luol@inspection.gc.ca
Sabara
M I
MI
Li
Y
Y
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood / Q000276:immunology; D000918:Antibody Specificity; D000956:Antigens, Viral / Q000276:immunology*; D015153:Blotting, Western / Q000662:veterinary; D002478:Cells, Cultured; D002645:Chickens / Q000821:virology; D003429:Cross Reactions; D006801:Humans; D029121:Metapneumovirus / Q000145:classification / Q000235:genetics / Q000276:immunology*; D018184:Paramyxoviridae Infections / Q000662:veterinary* / Q000821:virology; D010802:Phylogeny; D011201:Poultry Diseases / Q000175:diagnosis / Q000276:immunology / Q000821:virology; D011993:Recombinant Fusion Proteins; D014422:Turkeys / Q000821:virology
D000914:Antibodies, Viral; D000956:Antigens, Viral; D011993:Recombinant Fusion Proteins