[Prognosis of affinity change of the TATA-binding protein to TATA-boxes upon polymorphisms of the human gene promoter TATA boxes].
TATA-binding protein (TBP) is a subunit of basal transcription factor TFIID that recognizes and binds to the TATA-box on TATA-containing promoters of class II genes, and starts assembling RNA polymerase II basal transcription complex. It is shown in many works that the sequence of TATA-box with its flanking regions affects the level of basal and activated transcription. TATA-box polymorphisms and human hereditary diseases associated with them show that TBP/TATA interaction may indirectly affect gene regulation in vivo. The object of this work is to determine changes in the TBP/TATA affinity upon polymorphisms in TATA-boxes of human gene promoters. We assess changes in TBP/TATA affinities in silico by using our formula of equilibrium TBP/TATA binding upon four consecutive steps: nonspecific binding <--> sliding <--> braking (stopping) <--> stabilization. Our prognoses agree with known examples of TATA-box polymorphisms and human hereditary diseases associated with them.
Ponomarenko
P M
PM
Ponomarenko
M P
MP
Drachkova
I A
IA
Lysova
M V
MV
Arshinova
T V
TV
Savinkova
L K
LK
Kolchanov
N A
NA
D030342:Genetic Diseases, Inborn / Q000235:genetics; D020022:Genetic Predisposition to Disease; D006801:Humans; D008957:Models, Genetic; D020641:Polymorphism, Single Nucleotide; D016385:TATA Box; D035181:TATA-Box Binding Protein / Q000737:chemistry*
D035181:TATA-Box Binding Protein