title: Interleukin-6 expression on the biliary epithelia during inflammation-associated biliary carcinogenesis in bilioenterostomized hamsters.
abstract: BACKGROUND
  Chronic inflammatory conditions of the biliary tree strongly predispose patients to biliary carcinoma. The aim of this study was to evaluate the role of interleukin-6 (IL-6) expression during biliary carcinogenesis in bilioenterostomized hamsters.
  
  
  METHODS
  Syrian hamsters were subjected to either a choledochoduodenostomy (CD, n=11) or a simple laparotomy (SL, n=10) and then received N-nitrosobis(2-oxopropyl)amine (BOP) treatment. The animals were sacrificed 20 wk after surgery and the development of biliary carcinoma, the presence and degree of cholangitis, and IL-6 expression on the biliary epithelia were examined histologically.
  
  
  RESULTS
  In the CD group, eight hamsters (73%) demonstrated persistent cholangitis and six (55%) of them developed intrahepatic biliary carcinoma, while no hamster without cholangitis showed any biliary carcinoma. In the SL group, cholangitis was recognized in four hamsters (40%) and no development of biliary carcinoma was identified. A significantly high incidence of tumor development (P=0.024) and a close correlation between the presence of cholangitis and the occurrence of biliary carcinoma (P =0.013) were thus evident in the CD group. Moreover, the degree of cholangitis was significantly higher in the CD hamsters (P=0.041) and an IL-6 overexpression was identified in five hamsters that had undergone a CD, with a scattered expression on the intra- and extrahepatic biliary epithelia. Despite the fact that the induced biliary carcinomas showed a multicentric occurrence in the liver, these tumors originated from within the restricted area where IL-6 was expressed.
  
  
  CONCLUSIONS
  A deregulated IL-6 overexpression on the biliary epithelia may therefore be involved in inflammation-associated biliary carcinogenesis in hamsters that have undergone a bilioenterostomy.
authors: Department of Surgery, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Japan.
authors: Tomoo
authors: T
authors: Kitajima
authors: Yoshitsugu
authors: Y
authors: Tajima
authors: Tamotsu
authors: T
authors: Kuroki
authors: Noritsugu
authors: N
authors: Tsuneoka
authors: Tomohiko
authors: T
authors: Adachi
authors: Takashi
authors: T
authors: Kanematsu
mesh_terms: D000818:Animals; D001650:Bile Duct Neoplasms / Q000209:etiology / Q000378:metabolism*; D001659:Biliary Tract / Q000378:metabolism*; D002277:Carcinoma / Q000209:etiology / Q000378:metabolism*; D002761:Cholangitis / Q000150:complications*; D006224:Cricetinae; D004766:Enterostomy; D004848:Epithelium / Q000378:metabolism; D005260:Female; D015850:Interleukin-6 / Q000378:metabolism*; D008647:Mesocricetus
chemical_list: D015850:Interleukin-6