Raspberry-like assembly of cross-linked nanogels for protein delivery.
Raspberry-like assembly of nanogels (A-CHPNG) with a high potential as a carrier for protein delivery was prepared. Cross-linking of acrylate group-modified cholesterol-bearing pullulan nanogel (CHPANG) with thiol group-modified poly (ethylene glycol) (PEGSH) by Michael addition yielded A-CHPNG with narrow size distribution. The size of A-CHPNGs was controlled in the range of 40-120 nm by changing the concentration of CHPANG and PEGSH. A-CHPNG gradually degraded by hydrolysis under physiological condition and seemed to dissociate back to original nanogel. A-CHPNG encapsulated interleukin-12 (IL-12) efficiently (96%) and stably kept it in the presence of BSA (50 mg/ml). In addition, A-CHPNG had a high potential to maintain a high IL-12 level in plasma after subcutaneous injection in mice. Therefore, A-CHPNG is a promising carrier for long-term medications.
Hasegawa
Urara
U
Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, 2-3-10 Kanda-Surugadai, Chiyoda-ku, Tokyo 101-0062, Japan.
Sawada
Shin-Ichi
S
Shimizu
Takeshi
T
Kishida
Tsunao
T
Otsuji
Eigo
E
Mazda
Osam
O
Akiyoshi
Kazunari
K
D000179:Acrylates; D000818:Animals; D002784:Cholesterol / Q000737:chemistry; D003432:Cross-Linking Reagents; D004339:Drug Compounding; D016503:Drug Delivery Systems; D005936:Glucans / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D018664:Interleukin-12 / Q000096:biosynthesis / Q000235:genetics; D008027:Light; D051379:Mice; D018625:Microscopy, Atomic Force; D046529:Microscopy, Electron, Transmission; D011092:Polyethylene Glycols / Q000737:chemistry; D011506:Proteins / Q000008:administration & dosage*; D012542:Scattering, Radiation
D000179:Acrylates; D003432:Cross-Linking Reagents; D005936:Glucans; D011506:Proteins; D018664:Interleukin-12; D011092:Polyethylene Glycols; C009109:pullulan; D002784:Cholesterol