Roseomonas frigidaquae sp. nov., isolated from a water-cooling system.
A non-motile, coccobacilli-shaped, pale-pink-pigmented bacterium, designated strain CW67(T), was isolated from a water-cooling system in Gwangyang, Republic of Korea. Cells were found to be Gram-negative, catalase-positive and oxidase-positive, the major fatty acids were C(18 : 1)omega7c (43.6 %) and C(16 : 0) (15.8 %), the predominant respiratory lipoquinone was Q-10 and the DNA G+C content was 69.5 mol%. A phylogenetic tree based on 16S rRNA gene sequence comparisons showed that strain CW67(T) forms an evolutionary lineage within the radiation of the genus Roseomonas and that its closest relative is Roseomonas gilardii subsp. rosea MDA5605(T) (94.7 % sequence similarity). Evidence from this polyphasic study showed that strain CW67(T) could not be assigned to any recognized species. It therefore represents a novel species, for which the name Roseomonas frigidaquae sp. nov. is proposed, with CW67(T) (=KCTC 22211(T) =JCM 15073(T)) as the type strain.
Kim
Mi Sun
MS
Department of Biology, College of Life Science and Natural Resources, Sunchon National University, Suncheon 540-742, Republic of Korea.
Baik
Keun Sik
KS
Park
Seong Chan
SC
Rhee
Moon Soo
MS
Oh
Hee-Mock
HM
Seong
Chi Nam
CN
D016947:Acetobacteraceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology; D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000032:analysis; D005227:Fatty Acids / Q000032:analysis; D005618:Fresh Water / Q000382:microbiology*; D020459:Genes, rRNA; D007723:Korea; D008969:Molecular Sequence Data; D010641:Phenotype; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012034:Refrigeration; D017422:Sequence Analysis, DNA; D013045:Species Specificity
D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S