title: A murine model for studying diffusely injected human fat.
abstract: BACKGROUND
  The study of human autologous fat grafting has been primarily anecdotal. In this study, the authors aim to develop a murine model that recapitulates human fat grafting to study the fate of injected fat and the cell populations contained within.
  
  
  METHODS
  The authors' method of fat harvesting and refinement has been described previously. The authors injected nude and tie2/lacZ mice with 2 ml of human lipoaspirate placed on the dorsal surface in a multipass, fan-like pattern. Fatty tissue was injected in small volumes of approximately 1/30 ml per withdrawal. The dorsal skin and associated fat was excised at various time points. Sections were stained with hematoxylin and eosin and cytochrome c oxidase IV. Transgenic tie2/lacZ samples were stained with X-galactosidase. At the 8-week time point, volumetric analysis was performed.
  
  
  RESULTS
  Volumetric analysis at the 8-week time point showed 82 percent persistence of the original volume. Gross analysis showed it to be healthy, nonfibrotic, and vascularized. Hematoxylin and eosin analysis showed minimal inflammatory or capsular reaction, with viable adipocytes. Fat grafted areas were vascularized with multiple blood vessels. Cytochrome c oxidase IV human-specific stain and beta-galactosidase expression revealed these vessels to be of human origin.
  
  
  CONCLUSIONS
  The authors have developed a murine model with which to study the fate of injected lipoaspirate. There is a high level of persistence of the grafted human fat, with minimal inflammatory reaction. The fat is viable and vascularized, demonstrating human-derived vessels in a mouse model. This model provides a platform for studying the populations of progenitor cells known to reside in lipoaspirate.
authors: New York, N.Y. From the New York University School of Medicine.
authors: Vishal D
authors: VD
authors: Thanik
authors: Christopher C
authors: CC
authors: Chang
authors: Oren Z
authors: OZ
authors: Lerman
authors: Robert J
authors: RJ
authors: Allen
authors: Phuong D
authors: PD
authors: Nguyen
authors: Pierre B
authors: PB
authors: Saadeh
authors: Stephen M
authors: SM
authors: Warren
authors: Jamie P
authors: JP
authors: Levine
authors: Sydney R
authors: SR
authors: Coleman
authors: Alexes
authors: A
authors: Hazen
mesh_terms: D000273:Adipose Tissue / Q000637:transplantation*; D000818:Animals; D006801:Humans; D007267:Injections; D008297:Male; D051379:Mice; D008819:Mice, Nude; D023421:Models, Animal