MaxiK potassium channels in the function of chemoreceptor cells of the rat carotid body.
Hypoxia activates chemoreceptor cells of the carotid body (CB) promoting an increase in their normoxic release of neurotransmitters. Catecholamine (CA) release rate parallels the intensity of hypoxia. Coupling of hypoxia to CA release requires cell depolarization, produced by inhibition of O(2)-regulated K(+) channels, and Ca(2+) entering the cells via voltage-operated channels. In rat chemoreceptor cells hypoxia inhibits large-conductance, calcium-sensitive K channels (maxiK) and a two-pore domain weakly inward rectifying K(+) channel (TWIK)-like acid-sensitive K(+) channel (TASK)-like channel, but the significance of maxiK is controversial. A proposal envisions maxiK contributing to set the membrane potential (E(m)) and the hypoxic response, but the proposal is denied by authors finding that maxiK inhibition does not depolarize chemoreceptor cells or alters intracellular Ca(2+) concentration or CA release in normoxia or hypoxia. We found that maxiK channel blockers (tetraethylammonium and iberiotoxin) did not modify CA release in rat chemoreceptor cells, in either normoxia or hypoxia, and iberiotoxin did not alter the Ca(2+) transients elicited by hypoxia. On the contrary, both maxiK blockers increased the responses elicited by dinitrophenol, a stimulus we demonstrate does not affect maxiK channels in isolated patches of rat chemoreceptor cells. We conclude that in rat chemoreceptor cells maxiK channels do not contribute to the genesis of the E(m), and that their full inhibition by hypoxia, preclude further inhibition by maxiK channel blockers. We suggest that full inhibition of this channel is required to generate the spiking behavior of the cells in acute hypoxia.
Gomez-Niño
Angela
A
Departamento de Bioquímica y Biología Molecular y Fisiología, Universidad de Valladolid, Superior de Investigaciones Científicas, 47005 Valladolid, Spain.
Obeso
Ana
A
Baranda
Jose Antonio
JA
Santo-Domingo
Jaime
J
Lopez-Lopez
Jose Ramon
JR
Gonzalez
Constancio
C
D000818:Animals; D002344:Carotid Body / Q000166:cytology*; D002395:Catecholamines / Q000378:metabolism; D002628:Chemoreceptor Cells / Q000378:metabolism*; D004140:Dinitrophenols; D051036:Large-Conductance Calcium-Activated Potassium Channels / Q000037:antagonists & inhibitors / Q000378:metabolism*; D009538:Nicotine / Q000494:pharmacology; D010047:Ovalbumin; D010455:Peptides / Q000494:pharmacology; D011817:Rabbits; D051381:Rats; D017207:Rats, Sprague-Dawley; D019789:Tetraethylammonium / Q000494:pharmacology
D002395:Catecholamines; D004140:Dinitrophenols; D051036:Large-Conductance Calcium-Activated Potassium Channels; D010455:Peptides; C045521:dinitrophenol-ovalbumin; D019789:Tetraethylammonium; D009538:Nicotine; C064719:iberiotoxin; D010047:Ovalbumin