title: Enhanced systemic immune reactivity to a Basal cell carcinoma associated antigen following photodynamic therapy.
abstract: OBJECTIVE
  Numerous preclinical studies have shown that local photodynamic therapy (PDT) of tumors enhances systemic antitumor immunity. However, other than single-case and anecdotal reports, this phenomenon has not been examined following clinical PDT. To determine whether PDT in a clinical setting enhances systemic recognition of tumor cells, we examined whether PDT of basal cell carcinoma resulted in an increased systemic immune response to Hip1, a tumor antigen associated with basal cell carcinoma.
  
  
  METHODS
  Basal cell carcinoma lesions were either treated with PDT or surgically removed. Blood was collected from patients immediately before or 7 to 10 days following treatment. Peripheral blood leukocytes were isolated from HLA-A2-expressing patients and reactivity to a HLA-A2-restricted Hip1 peptide was measured by INF-gamma ELISpot assay.
  
  
  RESULTS
  Immune recognition of Hip1 increased in patients whose basal cell carcinoma lesions were treated with PDT. This increase in reactivity was significantly greater than reactivity observed in patients whose lesions were surgically removed. Patients with superficial lesions exhibited greater enhancement of reactivity compared with patients with nodular lesions. Immune reactivity following PDT was inversely correlated with treatment area and light dose.
  
  
  CONCLUSIONS
  These findings show for the first time that local tumor PDT can enhance systemic immune responses to tumors in patients, and validate previous preclinical findings.
authors: Authors' Affiliations: PDT Center, Department of Cell Stress Biology, Department of Dermatology, Department of Biostatistics, Roswell Park Cancer Center, Buffalo, New York.
authors: Edith
authors: E
authors: Kabingu
authors: Allan R
authors: AR
authors: Oseroff
authors: Gregory E
authors: GE
authors: Wilding
authors: Sandra O
authors: SO
authors: Gollnick
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000595:Amino Acid Sequence; D000951:Antigens, Neoplasm / Q000276:immunology*; D002280:Basal Cell Carcinoma / Q000188:drug therapy* / Q000276:immunology*; D002648:Child; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000276:immunology / Q000378:metabolism; D018604:Epitope Mapping; D005260:Female; D015789:HLA-A2 Antigen / Q000378:metabolism; D006801:Humans; D007111:Immunity, Cellular / Q000502:physiology*; D008297:Male; D008875:Middle Aged; D010446:Peptide Fragments / Q000032:analysis / Q000276:immunology / Q000378:metabolism; D010778:Photochemotherapy; D011485:Protein Binding / Q000276:immunology; D012878:Skin Neoplasms / Q000188:drug therapy* / Q000276:immunology*; D015854:Up-Regulation / Q000276:immunology; D055815:Young Adult
chemical_list: D000951:Antigens, Neoplasm; D004268:DNA-Binding Proteins; C073368:HIP1 protein, human; D015789:HLA-A2 Antigen; D010446:Peptide Fragments