[Effects of alginate on Th1/Th2 cytokines in vivo and in vitro of mice spleen lymphocytes].
OBJECTIVE
To study effects of alginate on Th1/Th2 cytokines in vivo and in vitro of mice spleen lymphocytes.


METHODS
In vivo test: Fifteen C57BL/6 mice were randomly divided into one negative control group and two Alginate groups, which were respectively given purified water or 0.50, 1.50 g/kg bw alginate everyday. After 30 days, mice spleen lymphocytes were separated and cultured for 48h. Culture supernatant were gathered for analysis of IL-2, IL-4, IL-5, TNF and IFN-gamma with cytometric bead array (CBA). In vitro test: C57BL/6 mice spleen lymphocytes were separated and respectively cultured with 25 mg/ml or 50 mg/ml alginate for 48h, and then culture supernatants were gathered for analysis of IL-2, IL-4, IL-5, TNF and IFN-gamma with cytometric bead array (CBA).


RESULTS
In vivo test: Compared with the negative control, the IFN-gamma of 0.5 g/kg bw and 1.5 g/kg bw alginate groups showed marked elevation (P < 0.01), while IL-2 and IL-4 were both decreased (P < 0.05, P < 0.01). In vitro test: Compared with control group, TNF, IL-2 and IL-4 of 25 mg/ml and 50 mg/ml alginate groups (P < 0.05, P < 0.01) and IL-5 of 50 mg/ml alginate group (P < 0.01) were all increased.


CONCLUSIONS
No matter in vivo or in vitro, alginate both can affect the Th1/Th2 cytokines of mice spleen lymphocytes.
Huang
Qiong
Q
Center for Disease Control and Prevention of Guangdong Province, Guangzhou 510300, China. huangqiong18@yahoo.com.cn
Huang
Junming
J
Yang
Xingfen
X
Cai
Min
M
D000464:Alginates / Q000494:pharmacology*; D000818:Animals; D016207:Cytokines / Q000276:immunology*; D005260:Female; D020723:Glucuronic Acid / Q000494:pharmacology; D006603:Hexuronic Acids / Q000494:pharmacology; D008214:Lymphocytes / Q000276:immunology* / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D013154:Spleen / Q000166:cytology; D018417:Th1 Cells / Q000276:immunology*; D018418:Th2 Cells / Q000276:immunology*
D000464:Alginates; D016207:Cytokines; D006603:Hexuronic Acids; D020723:Glucuronic Acid