Antisense interleukin-5 reduces eosinophil infiltration and hyperresponsiveness in an allergic asthma model.
Interleukin-5 (IL-5) involves in the development of airway inflammation and hyperresponsiveness through activation of eosinophils. Thus, inhibition of IL-5 expression seems to be an attractive approach for asthma therapy. In this study, an antisense IL-5 gene transferred by recombinant adeno-associated virus (asIL-5) was constructed to transfect murine allergic asthma model. Our results showed that asIL-5 efficiently inhibited the IL-5 mRNA expression and significantly attenuated the inflammation in lung tissues. Significant decreasing of eosinophils and inflammatory cells were found in peripheral blood and bronchoalveolar lavage fluid (BALF). In addition, significant inhibition of airway hyperresponsiveness (AHR) was also found in the mice treated with asIL-5. These observations demonstrate that antisense oligonucleotid against IL-5 delivered by adeno-associated virus system is possibly an efficacious therapeutic strategy for allergic asthma and other eosinophil-related disorders.
Huang
Feng-ying
FY
Hainan Provincial Key Laboratory of Tropical Medicine, Hainan Medical College, Haikou 571101, the People's Republic of China.
Wang
Cai-chun
CC
Zhou
Song-lin
SL
Huang
Yong-hao
YH
Wang
Hua
H
Chen
Fan
F
Lin
Ying-ying
YY
Tan
Guang-hong
GH
Liu
Jun-bao
JB
D000818:Animals; D001249:Asthma / Q000235:genetics / Q000276:immunology / Q000628:therapy*; D002465:Cell Movement / Q000276:immunology*; D000229:Dependovirus; D004195:Disease Models, Animal; D004804:Eosinophils / Q000276:immunology*; D006801:Humans; D015848:Interleukin-5 / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000276:immunology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D020319:Oligodeoxyribonucleotides, Antisense; D014161:Transduction, Genetic
D015848:Interleukin-5; D020319:Oligodeoxyribonucleotides, Antisense