title: Proteomic studies of rat tibialis anterior muscle during postnatal growth and development.
abstract: In this study, a proteomic analysis consisting of two-dimensional gel electrophoresis and MALDI-TOF/TOF mass spectrometry was accomplished to investigate the complex protein expression patterns in rat tibialis anterior muscle during postnatal 3-month period. We determined the time-dependent expression alterations of 107 protein spots, among which 53 protein spots were identified. These identified proteins included skeletal contractile proteins, metabolic enzymes, chaperone, intermediate filament, and signal transduction proteins. The time-dependent expression of three proteins, such as Mylpf, desmin, and RKIP, was confirmed by Western blot analysis and immunohistochemistry. The functional implication of these expression changes was also discussed. We further analyzed the linkage and interactions among the differentially expressed proteins (MAPK1, RKIP, AHSG, etc.). Collectively, the results might add to the understanding of the molecular mechanisms regulating postnatal growth and development of rat tibialis anterior muscle.
authors: Jiangsu Key Laboratory of Neuroregeneration, Nantong University, 19 Qi-Xiu Road, Nantong, Jiangsu Province, 226001, People's Republic of China.
authors: Hualin
authors: H
authors: Sun
authors: Ting
authors: T
authors: Zhu
authors: Fei
authors: F
authors: Ding
authors: Nan
authors: N
authors: Hu
authors: Xiaosong
authors: X
authors: Gu
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D015153:Blotting, Western; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D007124:Immunoenzyme Techniques; D009124:Muscle Proteins / Q000378:metabolism*; D018482:Muscle, Skeletal / Q000254:growth & development* / Q000378:metabolism*; D020543:Proteome / Q000032:analysis*; D040901:Proteomics / Q000379:methods*; D051381:Rats; D017207:Rats, Sprague-Dawley; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D009124:Muscle Proteins; D020543:Proteome