title: Molecular variation analysis of porcine reproductive and respiratory syndrome virus in China.
abstract: Porcine reproductive and respiratory syndrome virus (PRRSV) is characteristic of genetically extensive variation. The objective of the present study was to analyze the molecular variation and evolution of porcine reproductive and respiratory syndrome virus in China based on the complete genomic sequences of three highly pathogenic Chinese PRRSV strains isolated in 2006 and the sequences of the amplified Nsp2, ORF5 and ORF7 genes from clinical specimens during 2006-2008. Full-length genome sequencing and phylogenetic analysis showed that the three strains (JXwn06, BJsy06 and NX06) had a unique 30-amino-acid discontinuous deletion in Nsp2, and were classified into the same subgroup that consisted of the most Chinese strains isolated during 2006-2007, the pandemic period of atypical PRRS. The evolution analysis suggested that the emergence of the highly pathogenic PRRSV in China experienced a gradual variation and evolution accumulation progress from Chinese domestic virus. The variation analysis of the amplified 41 Nsp2, 59 ORF5 and 59 ORF7 genes indicated that the diversity of PRRSV strain existed in the field, and the highly pathogenic PRRSV strain with the 30-amino-acid deletion in Nsp2 was the dominating virus in China in recent years. Our data contribute to the understanding of molecular variation and epidemiology surveillance of PRRSV in China.
authors: Key Laboratory of Zoonosis of Ministry of Agriculture, College of Veterinary Medicine and State Key Laboratory of Agrobiotechnology, China Agricultural University, Beijing, PR China.
authors: Lei
authors: L
authors: Zhou
authors: Shuxian
authors: S
authors: Chen
authors: Jialong
authors: J
authors: Zhang
authors: Jingwen
authors: J
authors: Zeng
authors: Xin
authors: X
authors: Guo
authors: Xinna
authors: X
authors: Ge
authors: Dabing
authors: D
authors: Zhang
authors: Hanchun
authors: H
authors: Yang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002681:China / Q000453:epidemiology; D003546:Cysteine Endopeptidases / Q000235:genetics; D019143:Evolution, Molecular; D014644:Genetic Variation; D016679:Genome, Viral; D008969:Molecular Sequence Data; D010802:Phylogeny; D019318:Porcine Reproductive and Respiratory Syndrome / Q000453:epidemiology* / Q000821:virology; D019316:Porcine respiratory and reproductive syndrome virus / Q000235:genetics* / Q000302:isolation & purification; D012367:RNA, Viral / Q000032:analysis / Q000235:genetics; D016415:Sequence Alignment; D017423:Sequence Analysis, RNA; D013552:Swine
chemical_list: D012367:RNA, Viral; D003546:Cysteine Endopeptidases; C078306:nsP2 proteinase