title: Signalling mechanisms in mouse bitter responsive taste cells.
abstract: To clarify the components involved in mouse intracellular bitter transduction in the mouse, we investigated the expression patterns of transduction components and the contribution of intracellular Ca2+ stores. Of 64 cells responding to bitter compounds, 63% of the cells expressed the taste tissue-specific G-protein alpha-gustducin. Of the alpha-gustducin immunoreactive cells, 78% expressed IP3R3, a receptor that mediates Ca2+ release from the intracellular store. When intracellular Ca2+ was depleted by treatment with the inhibitor thapsigargin, 83% of the taste cells lost their response to bitter. These data suggest that bitterness transduction involves the release of Ca2+ from intracellular stores.
authors: Graduate School of Agriculture, Kyoto University, Kyoto, Japan.
authors: Masataka
authors: M
authors: Narukawa
authors: Eiko
authors: E
authors: Minamisawa
authors: Yukako
authors: Y
authors: Hayashi
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism; D020013:Calcium Signaling / Q000502:physiology*; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005260:Female; D020962:Heterotrimeric GTP-Binding Proteins / Q000378:metabolism; D053496:Inositol 1,4,5-Trisphosphate Receptors / Q000378:metabolism; D007424:Intracellular Fluid / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D000644:Quaternary Ammonium Compounds / Q000494:pharmacology; D015398:Signal Transduction / Q000502:physiology*; D013649:Taste / Q000502:physiology*; D013650:Taste Buds / Q000166:cytology / Q000378:metabolism*; D019284:Thapsigargin / Q000494:pharmacology; D017833:Visceral Afferents / Q000187:drug effects / Q000502:physiology
chemical_list: D004791:Enzyme Inhibitors; D053496:Inositol 1,4,5-Trisphosphate Receptors; D000644:Quaternary Ammonium Compounds; C043414:denatonium; D019284:Thapsigargin; C524407:GNAT3 protein, mouse; D020962:Heterotrimeric GTP-Binding Proteins; D002118:Calcium