title: Decreased sputum mature dendritic cells in healthy smokers and patients with chronic obstructive pulmonary disease.
abstract: BACKGROUND
  Asthmatics who smoke have decreased pulmonary mature dendritic cells (DCs). Chronic obstructive pulmonary disease (COPD) patients have an increased amount of pulmonary immature DCs. We hypothesized that healthy smokers and patients with COPD have decreased pulmonary mature DCs.
  
  
  METHODS
  We identified sputum DCs expressing the maturation markers CD83 and DC-lysosome associated membrane protein (DC-LAMP) and DC subpopulations (i.e. myeloid and plasmacytoid DCs) by flow cytometry in healthy smokers before they entered a smoking cessation trial (n = 30), in the same smokers after 6 months of smoking cessation (n = 11) and in COPD patients (n = 28, 14 current and 14 ex-smokers). 12 healthy never-smokers served as controls. DC numbers were expressed as percentage of total sputum CD45(+) leukocytes.
  
  
  RESULTS
  CD83(+) and DC-LAMP(+) mature DCs were decreased in healthy smokers before they ceased smoking compared to after (p = 0.003 and p = 0.049, respectively) and in smokers before smoking cessation compared to never-smokers (p = 0.027 and p = 0.028, respectively). COPD patients, both current and ex-smokers, showed decreased CD83(+) mature DCs compared to never-smokers and smokers after cessation (p = 0.042 and p = 0.004, respectively).
  
  
  CONCLUSIONS
  Cigarette smoking and COPD per se are associated with a decrease in pulmonary mature DCs. We speculate that this reduction is involved in the immunopathogenesis of smoking-related respiratory disorders, such as COPD.
authors: Department of Thoracic Medicine, University Hospital of Heraklion, GR-71110 Heraklion, Greece. tsoumak@yahoo.gr
authors: Maria
authors: M
authors: Tsoumakidou
authors: Izolde
authors: I
authors: Bouloukaki
authors: Heleni
authors: H
authors: Koutala
authors: Katerina
authors: K
authors: Kouvidi
authors: Ioanna
authors: I
authors: Mitrouska
authors: Spyros
authors: S
authors: Zakynthinos
authors: Nikolaos
authors: N
authors: Tzanakis
authors: Peter K
authors: PK
authors: Jeffery
authors: Nikolaos M
authors: NM
authors: Siafakas
mesh_terms: D000328:Adult; D000368:Aged; D015703:Antigens, CD / Q000096:biosynthesis; D000943:Antigens, Differentiation / Q000276:immunology / Q000378:metabolism*; D002452:Cell Count; D003713:Dendritic Cells / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D005260:Female; D006801:Humans; D007136:Immunoglobulins / Q000096:biosynthesis; D056747:Immunomodulation; D017493:Leukocyte Common Antigens / Q000096:biosynthesis; D051907:Lysosomal Membrane Proteins / Q000096:biosynthesis; D008297:Male; D008562:Membrane Glycoproteins / Q000096:biosynthesis; D008875:Middle Aged; D029424:Pulmonary Disease, Chronic Obstructive / Q000209:etiology / Q000276:immunology* / Q000473:pathology / Q000503:physiopathology / Q000517:prevention & control; D012907:Smoking / Q000009:adverse effects / Q000276:immunology* / Q000473:pathology / Q000503:physiopathology / Q000628:therapy; D016540:Smoking Cessation; D013183:Sputum / Q000166:cytology / Q000276:immunology*; D000097802:CD83 Antigen
chemical_list: D015703:Antigens, CD; D000943:Antigens, Differentiation; D007136:Immunoglobulins; D017493:Leukocyte Common Antigens; D051907:Lysosomal Membrane Proteins; D008562:Membrane Glycoproteins; D000097802:CD83 Antigen