Chromatic two-photon excitation fluorescence imaging.
We report on a chromatic axial scanning method for two-photon excitation fluorescence imaging. Effective axial scanning is achieved by incorporating a Fresnel lens in the system, which has large chromatic aberration and can therefore focus the excitation beam to different axial positions depending on its wavelength. We experimentally demonstrated this technique and used it to image the cross-section of fluorescent microspheres.
Xu
Q
Q
Department of Electrical Engineering, The Pennsylvania State University, University Park, Pennsylvania, USA.
Shi
K
K
Yin
S
S
Liu
Z
Z