A surface-charge study on cellular-uptake behavior of F3-peptide-conjugated iron oxide nanoparticles.
Surface-charge measurements of mammalian cells in terms of Zeta potential are demonstrated as a useful biological characteristic in identifying cellular interactions with specific nanomaterials. A theoretical model of the changes in Zeta potential of cells after incubation with nanoparticles is established to predict the possible patterns of Zeta-potential change to reveal the binding and internalization effects. The experimental results show a distinct pattern of Zeta-potential change that allows the discrimination of human normal breast epithelial cells (MCF-10A) from human cancer breast epithelial cells (MCF-7) when the cells are incubated with dextran coated iron oxide nanoparticles that contain tumor-homing F3 peptides, where the tumor-homing F3 peptide specifically bound to nucleolin receptors that are overexpressed in cancer breast cells.
Zhang
Yu
Y
Department of Mechanical Engineering, University of California at Riverside, Riverside, CA 92521, USA.
Yang
Mo
M
Park
Ji-Ho
JH
Singelyn
Jennifer
J
Ma
Huiqing
H
Sailor
Michael J
MJ
Ruoslahti
Erkki
E
Ozkan
Mihrimah
M
Ozkan
Cengiz
C
D045744:Cell Line, Tumor; D003911:Dextrans / Q000378:metabolism; D005290:Ferric Compounds / Q000378:metabolism*; D006801:Humans; D018613:Microscopy, Confocal; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D010455:Peptides / Q000378:metabolism*; D055672:Static Electricity
D003911:Dextrans; D005290:Ferric Compounds; D010455:Peptides; C000499:ferric oxide