Identification of Vibrio harveyi isolated from diseased cultured wedge sole Dicologoglossa cuneata.
We report the first isolation of Vibrio harveyi from wedge sole Dicologoglossa cuneata. The pathogen was recovered from ulcers and internal organs of ailing cultured fish, from 7 different outbreaks between 2004 and 2006. The 15 isolates found were phenotypically characterized using biochemical tests and BIOLOG GN plates, which revealed high phenotypic diversity. Diagnosis was confirmed with PCR using V harveyi specific primers and partial 16S and 23S rRNA gene sequencing. A virulence evaluation of the isolates was also performed using fry and juvenile wedge sole. Significant mortalities were recorded by intraperitoneal injection; however, no mortalities were recorded by bath immersion.
López
Jose R
JR
IFAPA Centro Agua del Pino, Junta de Andalucía, 21450 Huelva, Spain. lasacias@yahoo.es
de la Roca
Elena
E
Núñez
Soledad
S
de la Herran
Roberto
R
Navas
José I
JI
Manchado
Manuel
M
Herrera
Marcelino
M
Toranzo
Alicia E
AE
D000818:Animals; D017756:Aquaculture; D005393:Fish Diseases / Q000382:microbiology*; D005412:Flatfishes / Q000382:microbiology*; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012338:RNA, Ribosomal, 23S / Q000235:genetics; D014733:Vibrio / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000472:pathogenicity
D012336:RNA, Ribosomal, 16S; D012338:RNA, Ribosomal, 23S