[Detection of C-terminal 30 bp-deletion mutation of latent membrane protein 1 of EBV in childhood lymphoma].
OBJECTIVE
To study C-terminal 30 bp-deletion mutation of latent membrane protein 1 of the virus from childhood lymphoma.


METHODS
Nested-PCR was used to amplify C-terminal of EBV-LMP1 from childhood lymphoma and non-lymphoma associated to EBV, including Hodgkin lymphoma (HL), non-Hodgkin lymphoma (NHL)and reactive hyperplasia of lymph node (RL). Sequence analysis was performed on the positive PCR product.


RESULTS
LMP1 with 30 bp deletion in C-terminal was detected in 11/25 HL,3/8 NHL and 5/15 RL cases respectively. There were no significant differences among HL, NHL and RL (P = 0.793). Sequence analysis showed that LMP1 detected in this study belongs to the following three subgroups: B95.8, China1 and China2.


CONCLUSIONS
LMP1 with 30 bp deletion in C-terminal widely existed in childhood HL,NHL and RL. There was no correlation between special types of LMP1 and the diseases. There were 3 LMP1 subgroups of EBV in children's lymphoma in the cases studied, including B95.8, China 1 and China 2.
Xie
Zheng-de
ZD
Virology Laboratory, BeiJing Children's Hospital Affiliated to Capital Medical University, Beijing 100045, China. zhengde_xie@hotmail.com
Zhou
Chun-ju
CJ
Wang
Lin
L
Shen
Kun-ling
KL
D001483:Base Sequence; D002648:Child; D017353:Gene Deletion; D004854:Herpesvirus 4, Human / Q000145:classification / Q000235:genetics*; D006801:Humans; D008223:Lymphoma / Q000235:genetics*; D008969:Molecular Sequence Data; D010802:Phylogeny; D016415:Sequence Alignment; D014763:Viral Matrix Proteins / Q000145:classification / Q000235:genetics*
C037662:EBV-associated membrane antigen, Epstein-Barr virus; D014763:Viral Matrix Proteins