A Bombyx mandarina mutant exhibiting translucent larval skin is controlled by the molybdenum cofactor sulfurase gene.
During the maintenance of the wild silkworm, Bombyx mandarina, a mutant phenotype exhibiting translucent skin was identified. Based on the crossing experiments with the domesticated silkworm, Bombyx mori, we found that the mutant was controlled by molybdenum cofactor sulfurase (MoCoS) gene. We designated the mutant ''Ozaki's translucent'' (og(Z)). We found a 2.1-kb deletion containing the transcription initiation site, exons 1 and 2, and the 5' end of exon 3 of the MoCoS gene. The transcript of the MoCoS gene was not detected in the og(Z) homozygote. We concluded that og(Z) is a complete loss-of-function allele generated by a disruption of the MoCoS gene.
Fujii
Tsuguru
T
Department of Agricultural and Environmental Biology, Graduate School of Agricultual and Life Sciences, The University of Tokyo, Bunkyo-ku, Tokyo, Japan.
Ozaki
Masataka
M
Masamoto
Takaaki
T
Katsuma
Susumu
S
Abe
Hiroaki
H
Shimada
Toru
T
D000818:Animals; D012831:Bombyx / Q000201:enzymology / Q000235:genetics* / Q000378:metabolism; D003001:Cloning, Molecular; D003067:Coenzymes / Q000378:metabolism; D003433:Crosses, Genetic; D005260:Female; D015971:Gene Expression Regulation, Enzymologic; D019476:Insect Proteins / Q000235:genetics* / Q000378:metabolism; D007814:Larva / Q000201:enzymology / Q000235:genetics / Q000378:metabolism; D008297:Male; D008667:Metalloproteins / Q000378:metabolism; D008969:Molecular Sequence Data; D000090006:Molybdenum Cofactors; D009154:Mutation; D011621:Pteridines / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D013466:Sulfurtransferases / Q000235:genetics* / Q000378:metabolism
D003067:Coenzymes; D019476:Insect Proteins; D008667:Metalloproteins; D000090006:Molybdenum Cofactors; D011621:Pteridines; D013466:Sulfurtransferases; C022261:molybdopterin