title: Survey of feline leukemia virus and feline coronaviruses in captive neotropical wild felids from Southern Brazil.
abstract: A total of 57 captive neotropical felids (one Leopardus geoffroyi, 14 Leopardus pardalis, 17 Leopardus wiedii, 22 Leopardus tigrinus, and three Puma yagouaroundi) from the Itaipu Binacional Wildlife Research Center (Refúgio Bela Vista, Southern Brazil) were anesthetized for blood collection. Feces samples were available for 44 animals, including one L. geoffroyi, eight L. pardalis, 14 L. wiedii, 20 L. tigrinus, and one P. yagouaroundi. Total DNA and RNA were extracted from blood and feces, respectively, using commercial kits. Blood DNA samples were evaluated by polymerase chain reaction (PCR) for feline leukemia virus (FeLV) proviral DNA, whereas reverse transcriptase-PCR was run on fecal samples for detection of coronavirus RNA. None of the samples were positive for coronaviruses. A male L. pardalis and a female L. tigrinus were positive for FeLV proviral DNA, and identities of PCR products were confirmed by sequencing. This is the first evidence of FeLV proviral DNA in these species in Southern Brazil.
authors: Instituto de Ciências Biomédicas, USP, Av. Prof. Lineu Prestes, 1374, 05508900, São Paulo, Brazil.
authors: Ana M S
authors: AM
authors: Guimaraes
authors: Paulo E
authors: PE
authors: Brandão
authors: Wanderlei
authors: W
authors: de Moraes
authors: Zalmir S
authors: ZS
authors: Cubas
authors: Leonilda C
authors: LC
authors: Santos
authors: Laura Y B
authors: LY
authors: Villarreal
authors: Rogério R
authors: RR
authors: Robes
authors: Fabiana M
authors: FM
authors: Coelho
authors: Mauricio
authors: M
authors: Resende
authors: Renata C F
authors: RC
authors: Santos
authors: Rosangela C
authors: RC
authors: Oliveira
authors: Mauricio
authors: M
authors: Yamaguti
authors: Lucas M
authors: LM
authors: Marques
authors: Renata L
authors: RL
authors: Neto
authors: Melissa
authors: M
authors: Buzinhani
authors: Regina
authors: R
authors: Marques
authors: Joanne B
authors: JB
authors: Messick
authors: Alexander W
authors: AW
authors: Biondo
authors: Jorge
authors: J
authors: Timenetsky
mesh_terms: D000818:Animals; D000835:Animals, Wild; D000836:Animals, Zoo; D001938:Brazil / Q000453:epidemiology; D018352:Coronavirus Infections / Q000175:diagnosis / Q000453:epidemiology / Q000662:veterinary*; D016765:Coronavirus, Feline / Q000302:isolation & purification; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics; D005243:Feces / Q000821:virology; D045989:Felidae / Q000821:virology*; D005260:Female; D005256:Leukemia Virus, Feline / Q000302:isolation & purification; D008297:Male; D016133:Polymerase Chain Reaction / Q000662:veterinary; D015995:Prevalence; D046011:Puma / Q000821:virology*; D012192:Retroviridae Infections / Q000175:diagnosis / Q000453:epidemiology / Q000662:veterinary*; D013045:Species Specificity; D014412:Tumor Virus Infections / Q000175:diagnosis / Q000453:epidemiology / Q000662:veterinary*
chemical_list: D004279:DNA, Viral