title: [Analysis of variant translocation der ins (17; 15) in patient with APL by G-banding technique and interphase fluorescence in situ hybridization].
abstract: To investigate the biological characteristics of the variant translocation der ins (17;15) in a patient with acute promyelocytic leukemia (APL), the conventional G-banding technique, interphase fluorescence in situ hybridization (int-FISH), RT-PCR, gene scanning, gene sequence and flow cytometry were performed. The results indicated that the variant translocation der ins (17, 15) observed by G banding technique was a rare type, the int-FISH assay by using dual-color pml/raralpha fusion probes confirmed the cytogenetic findings. The detection results of other molecular methods demonstrated the existence of the whole pml/raralpha fusion gene, while this case had insertion variant translocation. This patient got complete remission by using combined chemotherapy, and survives with continuous complete remission during following up for 1 year. In conclusion, the variant translocation der ins (17; 15) is rare type in APL, its incidence is lower, several signal types in detection of int-FISH were observed and the combination chemotherapy for this patient showed more obvious efficacy.
authors: Laboratory of Cytogenetics, Specific Examination Center, Beijing Daopei Hospital, Beijing, China.
authors: Tong
authors: T
authors: Wang
authors: Jing-Ying
authors: JY
authors: Qiu
authors: Chun-Fu
authors: CF
authors: Yu
authors: Xiao-Lan
authors: XL
authors: Ma
authors: Xiao-Peng
authors: XP
authors: Jia
authors: Yan-Ping
authors: YP
authors: Wang
authors: Hong-Xing
authors: HX
authors: Liu
authors: Yue-Hui
authors: YH
authors: Lin
authors: Chun-Rong
authors: CR
authors: Tong
authors: Dao-Pei
authors: DP
authors: Lu
mesh_terms: D002871:Chromosome Banding; D002884:Chromosomes, Human, Pair 15; D002886:Chromosomes, Human, Pair 17; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D007399:Interphase / Q000235:genetics; D015473:Leukemia, Promyelocytic, Acute / Q000235:genetics*; D008297:Male; D014178:Translocation, Genetic; D055815:Young Adult