title: Quantification of domoic acid in shellfish tissues by pressurized capillary electrochromatography.
abstract: A method was developed to quantify domoic acid (DA), the chemical responsible for amnesic shellfish poisoning (ASP), by pressurized CEC (pCEC). The effect of different experimental conditions on the separation of DA and matrix solutes, such as the content of ACN in mobile phase, pH and concentration of buffer, supplementary pressure and applied voltage, were investigated. Under the optimal conditions, the pCEC method separated DA from shellfish matrices within 6 min. By using supplementary pressure, bubble formation in the capillary column was completely suppressed. The method was repeatable, sufficient accurate and sensitive for rapid screening of DA in shell seafood.
authors: Department of Chemistry, Fuzhou University, Fuzhou, China.
authors: Weimin
authors: W
authors: Wu
authors: Xiaoping
authors: X
authors: Wu
authors: Xucong
authors: X
authors: Lin
authors: Zenghong
authors: Z
authors: Xie
authors: John P
authors: JP
authors: Giesy
mesh_terms: D000818:Animals; D002021:Buffers; D002138:Calibration; D053801:Capillary Electrochromatography / Q000295:instrumentation / Q000379:methods*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007608:Kainic Acid / Q000031:analogs & derivatives* / Q000032:analysis; D009467:Neuromuscular Depolarizing Agents / Q000032:analysis*; D009498:Neurotoxins / Q000032:analysis*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012758:Shellfish / Q000032:analysis*
chemical_list: D002021:Buffers; D009467:Neuromuscular Depolarizing Agents; D009498:Neurotoxins; C012301:domoic acid; D007608:Kainic Acid