title: Protein composition of oil bodies from mature Brassica napus seeds.
abstract: Seed oil bodies (OBs) are intracellular particles storing lipids as food or biofuel reserves in oleaginous plants. Since Brassica napus OBs could be easily contaminated with protein bodies and/or myrosin cells, they must be purified step by step using floatation technique in order to remove non-specifically trapped proteins. An exhaustive description of the protein composition of rapeseed OBs from two double-zero varieties was achieved by a combination of proteomic and genomic tools. Genomic analysis led to the identification of sequences coding for major seed oil body proteins, including 19 oleosins, 5 steroleosins and 9 caleosins. Most of these proteins were also identified through proteomic analysis and displayed a high level of sequence conservation with their Arabidopsis thaliana counterparts. Two rapeseed oleosin orthologs appeared acetylated on their N-terminal alanine residue and both caleosins and steroleosins displayed a low level of phosphorylation.
authors: NRA, UMR 206, Chimie Biologique, Thiverval-Grignon, France. jolivet@grignon.inra.fr
authors: Pascale
authors: P
authors: Jolivet
authors: Céline
authors: C
authors: Boulard
authors: Annick
authors: A
authors: Bellamy
authors: Colette
authors: C
authors: Larré
authors: Marion
authors: M
authors: Barre
authors: Hélène
authors: H
authors: Rogniaux
authors: Sabine
authors: S
authors: d'Andréa
authors: Thierry
authors: T
authors: Chardot
authors: Nathalie
authors: N
authors: Nesi
mesh_terms: D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000235:genetics; D029688:Brassica napus / Q000737:chemistry* / Q000235:genetics; D029741:Brassica rapa / Q000737:chemistry / Q000235:genetics; D002135:Calcium-Binding Proteins / Q000032:analysis; D015151:Immunoblotting; D002479:Inclusion Bodies / Q000737:chemistry*; D008969:Molecular Sequence Data; D010802:Phylogeny; D010940:Plant Proteins / Q000032:analysis; D011499:Protein Processing, Post-Translational; D055314:Seed Storage Proteins / Q000032:analysis* / Q000737:chemistry / Q000235:genetics; D012639:Seeds / Q000737:chemistry*; D016415:Sequence Alignment
chemical_list: D002135:Calcium-Binding Proteins; D010940:Plant Proteins; D055314:Seed Storage Proteins; C402836:caleosin protein, Sesamum indicum; C079356:oleosin protein, Brassica napus