Glucose-sensitivity of liposomes incorporating conjugates of glucose oxidase and poly(N-isopropylacrylamide-co-methacrylic acid-co-octadecylacrylate).
Liposomes, which release their contents in response to the concentration of glucose, were prepared by modifying the liposomal surface with the conjugate of poly(N-isopropylacrylamide-co-methacrylic acid-co-octadecylacrylate) (P(NIPAM-co-MAA-co-ODA)) and glucose oxidase (GOD). The maximum enzymatic activity of copolymer conjugated GOD (Polym-GOD) was observed around pH 5.0 and the value was about 40% of that of native GOD. Nine lysine residues per GOD molecule, on average, were found to be covalently attached to the copolymers. Egg phosphatidylcholine liposomes bearing Polym-GOD released their contents in response to the concentration of glucose and the sensitivity was higher than dipalmitoylphosphatidylcholine liposomes.
Jo
Seong-Min
SM
School of Biotechnology & Bioengineering and Institute of Bioscience and Biotechnology, Kangwon National University, 192-1, Hyoja 2-dong, Chunchon, Kangwon-do 200-701, Republic of Korea.
Lee
Hyeon Yong
HY
Kim
Jin-Chul
JC
D015060:1,2-Dipalmitoylphosphatidylcholine / Q000737:chemistry; D000180:Acrylic Resins / Q000737:chemistry*; D000388:Air; D004800:Enzymes, Immobilized / Q000737:chemistry / Q000378:metabolism; D005947:Glucose / Q000378:metabolism / Q000494:pharmacology*; D005949:Glucose Oxidase / Q000737:chemistry* / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D057927:Hydrophobic and Hydrophilic Interactions; D008081:Liposomes / Q000737:chemistry / Q000378:metabolism*; D046529:Microscopy, Electron, Transmission; D008968:Molecular Conformation; D011108:Polymers / Q000737:chemistry*; D013500:Surface Tension; D013535:Suspensions; D014867:Water / Q000737:chemistry
D000180:Acrylic Resins; D004800:Enzymes, Immobilized; D008081:Liposomes; D011108:Polymers; D013535:Suspensions; C546457:poly(N-isopropylacrylamide-co-methacrylic acid-co-octadecylacrylate); D014867:Water; D015060:1,2-Dipalmitoylphosphatidylcholine; D005949:Glucose Oxidase; D005947:Glucose