Development of a VX2 pancreatic cancer model in rabbits: a pilot study.
OBJECTIVE
An animal model of pancreatic cancer that is large enough to permit imaging and catheterization would be desirable for interventional radiologists to develop novel therapies for pancreatic cancer. The purpose of this study was to test the hypothesis that the VX2 rabbit model of pancreatic cancer could be developed as a suitable platform to test future interventional therapies.


METHODS
The authors implanted and grew three pancreatic VX2 tumors per rabbit in six rabbits. Magnetic resonance (MR) imaging was performed at 2 weeks to confirm tumor growth. At 3 weeks, the authors selectively catheterized the gastroduodenal artery under guidance of x-ray digital subtraction angiography (DSA). T2-weighted anatomic imaging, diffusion-weighted MR imaging, and transcatheter intraarterial perfusion (TRIP) MR imaging were then performed. After imaging, tumors were confirmed at necropsy and histopathologically. Tumor sizes at 2 and 3 weeks were compared with a paired t test (P = .05).


RESULTS
VX2 pancreatic tumors were grown in all six rabbits. The difference between tumor sizes at 2 and 3 weeks (1.29 cm +/- 0.39 vs 1.91 cm +/- 0.50, respectively) was significant (P < .001). All tumors were confirmed to be located within pancreatic tissue via histopathologic analysis. DSA and TRIP MR imaging were successful in five rabbits. Diffusion-weighted and anatomic MR imaging were successful in all six rabbits.


CONCLUSIONS
The VX2 rabbit model of pancreatic cancer is feasible, as verified by imaging and pathologic correlation, and may be a suitable platform to test future interventional therapies.
Eifler
Aaron C
AC
Department of Radiology, Feinberg School of Medicine, Northwestern University, 737 North Michigan Avenue, Chicago, IL 60611, USA.
Lewandowski
Robert J
RJ
Virmani
Sumeet
S
Chung
Johnathan C
JC
Wang
Dingxin
D
Tang
Richard L
RL
Szolc-Kowalska
Barbara
B
Woloschak
Gayle E
GE
Yang
Guang-Yu
GY
Ryu
Robert K
RK
Salem
Riad
R
Larson
Andrew C
AC
Cheon
Eric
E
Strouch
Matthew
M
Bentrem
David J
DJ
Omary
Reed A
RA
D000818:Animals; D045744:Cell Line, Tumor; D004195:Disease Models, Animal; D006801:Humans; D010190:Pancreatic Neoplasms / Q000000981:diagnostic imaging* / Q000601:surgery*; D010865:Pilot Projects; D011817:Rabbits; D015641:Radiography, Interventional / Q000379:methods*