title: Prevalence of respiratory syncytial virus group B genotype BA-IV strains among children with acute respiratory tract infection in Kolkata, Eastern India.
abstract: BACKGROUND
  Although recognized as a significant respiratory pathogen, there is a dearth of information on genetic diversity of human respiratory syncytial virus (HRSV) strains among children in India.
  
  
  OBJECTIVE
  To study prevalence and genomic diversity of HRSV strains among children (<5 years of age) with acute respiratory tract infection (ARTI) at Dr. B.C. Roy Memorial Hospital for Children, Kolkata, Eastern India.
  
  
  METHODS
  During September 2005 to August 2008, nasal and/or throat swabs from children with ARTI were screened for presence of HRSVs by RT-PCR of nucleocapsid (N) and glycoprotein (G) genes. Classification of G gene of HRSV strains were achieved with different primer pairs designed for amplification of N'- and/or C'-terminal hypervariable regions (HVR1 and HVR2) of HRSV A and B strains. The HVR1 and HVR2 of G gene of 27 and 22 HRSV B strains were sequenced.
  
  
  RESULTS
  One hundred seventy seven of 1720 clinical samples were positive for HRSVs. Group B strains were detected at higher rates (95% against 5%, n=80) than A in 2005-2006, whereas in consecutive years, the rate of detection of group A were higher (94.84% against 5.16%, n=97). The group B strains were genetically related to globally spreading BA genotype, exhibiting conserved nature of stop codon, six nucleotide deletions in HVR1 and formed single phylogenetic clusters for both HVR1 and HVR2.
  
  
  CONCLUSIONS
  The detection of significant high rates of group B strains in 2005-2006 followed by increase in prevalence of A strains in subsequent years highlight the dynamic nature of prevalence of HRSV subtypes among children with ARTI in Eastern India.
authors: Division of Virology, National Institute of Cholera and Enteric Diseases, P-33 C.I.T. Road, Scheme XM, Beliaghata, Kolkata 700010, India.
authors: Anurodh Shankar
authors: AS
authors: Agrawal
authors: Mehuli
authors: M
authors: Sarkar
authors: Souvik
authors: S
authors: Ghosh
authors: Mamta
authors: M
authors: Chawla-Sarkar
authors: Nilanjan
authors: N
authors: Chakraborty
authors: Meena
authors: M
authors: Basak
authors: Trailokya Nath
authors: TN
authors: Naik
mesh_terms: D002675:Child, Preschool; D016000:Cluster Analysis; D014644:Genetic Variation; D005838:Genotype; D006761:Hospitals; D006801:Humans; D007194:India / Q000453:epidemiology; D007223:Infant; D007231:Infant, Newborn; D017720:Molecular Epidemiology; D008969:Molecular Sequence Data; D010802:Phylogeny; D015995:Prevalence; D012367:RNA, Viral / Q000235:genetics; D018357:Respiratory Syncytial Virus Infections / Q000453:epidemiology* / Q000821:virology*; D018113:Respiratory Syncytial Virus, Human / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D012141:Respiratory Tract Infections / Q000453:epidemiology* / Q000821:virology*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D015678:Viral Structural Proteins / Q000235:genetics
chemical_list: D012367:RNA, Viral; D015678:Viral Structural Proteins