[Isolation, identification and clinical significance of ascites-derived exosomes from patients with ovarian epithelial cancer].
OBJECTIVE
To isolate and identify the ascites-derived exosomes from patients with ovarian epithelial carcinoma, and explore the relationship between exosomes and the prognosis of ovarian epithelial carcinoma.


METHODS
Ascites-derived exosomes were isolated by ultracentrifugation on sucrose and D2O gradients from 41 ovarian epithelial carcinoma patients, and identified by transmission electron microscope and western blot analyses. Ascites-derived exosomes were evaluated for effect on prognosis of ovarian epithelial carcinoma.


RESULTS
Exosomes were isolated and purified from the ascites in 85% (35/41) of ovarian epithelial carcinoma patients; major histocompability complex-I (MHC-I) could be detected in 100% (35/35) of the ascites-derived exosomes samples, heat shock protein-70 (Hsp70) in 91% (32/35), and CD(81) in 86% (30/35). The patients with positive ascites-derived exosomes had no significant difference in age, pathological type and the degree of differentiation of tumor, surgical-pathological staging, the optimal operation and the responsibility to chemotherapy (P > 0.05). The patients with positive ascites-derived exosomes, the reduction of CA(125) level after cytoreductive surgery was (66 +/- 27)%, which was more than that of the patients without ascites-derived exosomes (37 +/- 86)% and all the whole patients not considering the condition of exosomes (61 +/- 44)% (P < 0.01), and there were also no difference whether or no optimal operation ration (P < 0.01).


CONCLUSIONS
There are ascites-derived exosomes in ascites from most patients with ovarian epithelial carcinoma. While the relationship between exosomes and prognosis of ovarian epithelial carcinoma needs further research.
Peng
Peng
P
Department of Obstetrics and Gynecology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences, Beijing 100730, China.
You
Yan
Y
Shen
Keng
K
D000328:Adult; D000368:Aged; D001201:Ascites / Q000378:metabolism; D018394:CA-125 Antigen / Q000097:blood; D002498:Centrifugation / Q000379:methods; D018284:Cystadenocarcinoma, Serous / Q000378:metabolism / Q000473:pathology* / Q000601:surgery; D055354:Exosomes / Q000648:ultrastructure; D005260:Female; D018840:HSP70 Heat-Shock Proteins / Q000378:metabolism; D015395:Histocompatibility Antigens Class I / Q000378:metabolism; D006801:Humans; D046529:Microscopy, Electron, Transmission; D008875:Middle Aged; D009367:Neoplasm Staging; D010051:Ovarian Neoplasms / Q000378:metabolism / Q000473:pathology* / Q000601:surgery; D011379:Prognosis; D012189:Retrospective Studies
D018394:CA-125 Antigen; D018840:HSP70 Heat-Shock Proteins; D015395:Histocompatibility Antigens Class I