Toxoplasma gondii: P30 peptides recognition pattern in human toxoplasmosis.
In this study, human sera reactivity against nine peptides derived from the Toxoplasma gondii P30 protein was assessed by ELISA in patients with different clinical forms of toxoplasmosis. Same as has been reported in mice, sera from congenital, ocular and chronic asymptomatic toxoplasmosis patients recognized more strongly peptides from the protein's carboxy-terminus, being peptide 2017 (amino acids 301-320) the one most strongly recognized by sera from patients with ocular toxoplasmosis. Serum samples collected from 13 patients without ocular infection, 13 with inactive chorioretinal scars, 6 with active ocular infection and 10 seronegative individuals were then screened for anti-2017 IgG. Peptide 2017 was recognized by all patients' samples but not by sera from T. gondii-seronegative individuals. No statistically significant differences were found between the absorbance levels of groups with and without lesions or with active or inactive ocular lesions, as determined by ANOVA.
Cardona
Nestor
N
Centro de Investigaciones Biomedicas, Universidad del Quindio, Armenia, Colombia.
de-la-Torre
Alejandra
A
Siachoque
Heber
H
Patarroyo
Manuel A
MA
Gomez-Marin
Jorge E
JE
D000595:Amino Acid Sequence; D000704:Analysis of Variance; D000818:Animals; D000953:Antigens, Protozoan / Q000737:chemistry / Q000276:immunology*; D000954:Antigens, Surface / Q000737:chemistry / Q000276:immunology; D002908:Chronic Disease; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007106:Immune Sera / Q000276:immunology; D007074:Immunoglobulin G / Q000276:immunology; D007223:Infant; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry / Q000276:immunology; D015800:Protozoan Proteins / Q000737:chemistry / Q000276:immunology*; D014122:Toxoplasma / Q000737:chemistry / Q000276:immunology*; D014123:Toxoplasmosis / Q000276:immunology*; D014125:Toxoplasmosis, Congenital / Q000276:immunology; D014126:Toxoplasmosis, Ocular / Q000276:immunology
D000953:Antigens, Protozoan; D000954:Antigens, Surface; D007106:Immune Sera; D007074:Immunoglobulin G; D010455:Peptides; D015800:Protozoan Proteins; C047168:SAG1 antigen, Toxoplasma