title: [Comparative quantification of plasma hnRNP B1 mRNA in non-small cell lung cancer patients by real-time PCR].
abstract: BACKGROUND
  Circulating cell-free nucleic acids are known to be a noninvasive diagnostic tool for cancer detection. Heterogeneous nuclear ribonucleoprotein (hnRNP) B1, a nuclear core complex, is overexpressed in early stage lung cancer. We intended to evaluate the usefulness of plasma hnRNP B1 mRNA in differentiating non-small cell lung cancer (NSCLC) from other benign lung diseases, especially pulmonary tuberculosis, which is highly prevalent in Korea and often difficult to distinguish from lung cancer.
  
  
  METHODS
  Plasma RNA was extracted from 30 patients with NSCLC, 30 patients with benign lung diseases including pulmonary tuberculosis, and 10 healthy controls. Plasma hnRNP B1 mRNA was measured by TaqMan Gene Expression Assay (Applied Biosystems, USA), and pre-developed beta-actin (ACTB) mRNA was used for normalization. We analyzed the relative gene expression data using the delta-delta Ct method.
  
  
  RESULTS
  Plasma hnRPN B1 mRNA was measurable in 93.3% (28/30) of NSCLC patients. Normalized 2-DeltaDeltaCt of plasma hnRPN B1 mRNA was 62.2 (95%Cl, 6.4-210.1) in NSCLC patients and 2.7 (95%Cl, 0.5-13.6) in benign lung disease patients (P<0.001, Mann-Whitney U test).
  
  
  CONCLUSIONS
  Plasma hnRNP B1 mRNA was significantly increased in patients with lung cancer compared with that in patients with other benign lung diseases. Plasma hnRNP B1 mRNA may be useful as a potential marker for the detection of NSCLC.
authors: Department of Laboratory Medicine, Dong-A University College of Medicine, Seo-Gu, Busan, Korea.
authors: Jeong Man
authors: JM
authors: Kim
authors: Sang Hyun
authors: SH
authors: Hwang
authors: Eun Ju
authors: EJ
authors: Song
authors: Sang Yull
authors: SY
authors: Lee
authors: Yeong Dae
authors: YD
authors: Kim
authors: Chang Hun
authors: CH
authors: Lee
authors: Min Ki
authors: MK
authors: Lee
authors: Chulhun L
authors: CL
authors: Chang
authors: Eun Yup
authors: EY
authors: Lee
mesh_terms: D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor; D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics*; D005260:Female; D034481:Heterogeneous-Nuclear Ribonucleoprotein Group A-B / Q000097:blood* / Q000235:genetics; D006801:Humans; D008171:Lung Diseases / Q000097:blood / Q000235:genetics; D008175:Lung Neoplasms / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000097:blood* / Q000378:metabolism
chemical_list: D014408:Biomarkers, Tumor; D034481:Heterogeneous-Nuclear Ribonucleoprotein Group A-B; D012333:RNA, Messenger; C439768:hnRNP A2