title: AasP autotransporter protein of Actinobacillus pleuropneumoniae does not protect pigs against homologous challenge.
abstract: Actinobacillus pleuropneumoniae is a major respiratory pathogen of pigs; current vaccines provide only limited protection. AasP, a subtilisin-like serine protease, is a conserved outer membrane-localised autotransporter protein. We hypothesized that AasP would induce protective immunity and may thus constitute a useful component of a vaccine against A. pleuropneumoniae infection. Here we confirm experimentally that AasP is an antigenic in vivo-expressed protein. In pig protection studies, a detectable specific antibody response was induced in response to recombinant AasP. However, the vaccine was not capable of protecting pigs from colonization, infection or severe clinical disease resulting from challenge with the homologous A. pleuropneumoniae strain.
authors: Molecular Bacteriology and Immunology Group, Institute of Infection, Immunity & Inflammation, Centre for Biomolecular Sciences, University of Nottingham, University Park, Nottingham NG7 2RD, United Kingdom.
authors: Neil J
authors: NJ
authors: Oldfield
authors: Kathryn E
authors: KE
authors: Worrall
authors: Andrew N
authors: AN
authors: Rycroft
authors: Tehmeena
authors: T
authors: Ali
authors: Karl G
authors: KG
authors: Wooldridge
authors: Dlawer A A
authors: DA
authors: Ala'Aldeen
mesh_terms: D000189:Actinobacillus Infections / Q000276:immunology / Q000517:prevention & control / Q000662:veterinary*; D016977:Actinobacillus pleuropneumoniae / Q000276:immunology*; D000595:Amino Acid Sequence; D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood / Q000276:immunology; D001425:Bacterial Outer Membrane Proteins / Q000276:immunology*; D001428:Bacterial Vaccines / Q000276:immunology*; D017124:Conserved Sequence; D008969:Molecular Sequence Data; D011994:Recombinant Proteins / Q000276:immunology; D013381:Subtilisins / Q000276:immunology; D013552:Swine / Q000276:immunology* / Q000382:microbiology; D013553:Swine Diseases / Q000276:immunology / Q000517:prevention & control*
chemical_list: D000907:Antibodies, Bacterial; D001425:Bacterial Outer Membrane Proteins; D001428:Bacterial Vaccines; D011994:Recombinant Proteins; D013381:Subtilisins