Interleukin-17 and kidney allograft outcome.
Acute rejection episodes (ARE) are important complications that involve the interplay between mechanisms that maintain graft tolerance and promote rejection. The proinflammatory cytokine interleukin-17 (IL-17) has been implicated in many conditions in humans and mice. In kidney transplant patients, the evaluation IL-17 levels has been performed in only a few patients. We performed a cross-sectional study correlating quantitative IL-17 levels and clinical outcomes.


METHODS
We studied 19 specimens from biopsies performed in patients (n = 19) who received isolated kidney grafts. ARE signs were present in 9 (47%) patients who provide specimens; whereas, 10 (53%) others showed no signs of rejection. Eighteen healthy control sample IL-17 underwent measurement, all of which were performed by an enzyme-linked immunosorbent assay method. We assessed other factors, such as the recipients demographic data, cold ischemia time, HLA mismatches, time elapsed from transplantation to the biopsy, posttransplantation status, antibody panel, donor type, and immunosuppressive treatment.


RESULTS
IL-17 levels were clearly increased among samples derived from patients with ongoing rejection (125.7 +/- 27.06 pg/mL) in contrast, to the nonrejection group, (30 +/- 13.32 pg/mL) (P < .05). Healthy controls showed no detectable IL-17 levels.


CONCLUSIONS
These findings suggested that IL-17 was important in the pathophysiology of acute kidney rejection.
Crispim
J C O
JC
Department of Biochemistry and Immunology, School of Medicine of Ribeirão Preto, University of São Paulo, (FMRP-USP), São Paulo, Brazil. janacrispim@usp.br
Grespan
R
R
Martelli-Palomino
G
G
Rassi
D M
DM
Costa
R S
RS
Saber
L T
LT
Cunha
F Q
FQ
Donadi
E A
EA
D000328:Adult; D000818:Animals; D015415:Biomarkers / Q000097:blood*; D001706:Biopsy; D005260:Female; D006084:Graft Rejection / Q000097:blood* / Q000276:immunology; D006801:Humans; D007166:Immunosuppressive Agents / Q000627:therapeutic use; D020381:Interleukin-17 / Q000097:blood*; D008297:Male; D051379:Mice; D008875:Middle Aged; D012016:Reference Values
D015415:Biomarkers; D007166:Immunosuppressive Agents; D020381:Interleukin-17