title: [Influence of PAHs exposure and GSTT1, GSTM1 genotypes on urinary 1-OHP as exposure biomarker].
abstract: OBJECTIVE
  To study the influence of polycyclic aromatic hydrocarbons (PAHs) exposure (ambient concentration and smoking) and GSTT1, GSTM1, genotypes on urinary 1-OHP as exposure biomarker.
  
  
  METHODS
  51 Cops who working outside were selected as study group, 48 Cops who working in office were selected as control Group. The ambient concentrations of PAHs both of two circumstances and the urinary 1-OHP of subjects were measured. The ambient concentrations of PAHs and the urinary 1-OHP were determined by high performance liquid chromatography (HPLC). The GSTT1, GSTM1 genotypes were determined by PCR.
  
  
  RESULTS
  The ambient concentrations of PAHs in the study group and the control group were 20.85 ng/m3 and 12.79 ng/m3 respectively. There were not significant difference in urinary 1-OHP among the persons who had the deference GSTT1, GSTM1, genotypes and the same smoking habits in the study group and the control group. The urinary 1-OHP concentrations of the study group were higher than those of the control group when they had the same genotypes for non-smokers. Smoking stratified analysis showed that urinary 1-OHP concentrations of smokers were higher than those of non-smokers, the urinary 1-OHP concentrations of smokers in control group were higher than those of the non-smokers in study group.
  
  
  CONCLUSIONS
  The ambient PAHs concentration and smoking could be the important factors generated the urinary 1-OHP concentration. While the levels of ambient PAHs were low, the smoking could change more prominent. The GSTT1, GSTM1 genotypes could not be the major influential factors.
authors: Institute for Environmental Health and Related Product Safety, Chinese Center for Disease Control and Prevention, Beijing 100021, China. junruichang@yahoo.com.cn
authors: Junrui
authors: J
authors: Chang
authors: Dongqun
authors: D
authors: Xu
authors: Wenjie
authors: W
authors: Zhang
authors: Changming
authors: C
authors: Ding
mesh_terms: D000328:Adult; D015415:Biomarkers / Q000652:urine; D004781:Environmental Exposure / Q000009:adverse effects*; D005260:Female; D005838:Genotype; D005982:Glutathione Transferase / Q000235:genetics*; D006801:Humans; D008297:Male; D016273:Occupational Exposure / Q000009:adverse effects; D011084:Polycyclic Aromatic Hydrocarbons / Q000633:toxicity*; D011721:Pyrenes / Q000378:metabolism*
chemical_list: D015415:Biomarkers; D005982:Glutathione Transferase; D011084:Polycyclic Aromatic Hydrocarbons; D011721:Pyrenes; C413545:glutathione S-transferase T1; C033146:1-hydroxypyrene