Characterization of new types of stationary phases for fast liquid chromatographic applications.
The performance of a narrow bore silica based monolith column (5 cm x 2 mm) was compared to 5 cm long narrow bore (internal diameter < or = 2.1 mm) columns, packed with shell particles (2.7 microm) and totally porous sub-2 microm particles (1.5 microm, 1.7 microm and 1.9 microm) in gradient and isocratic elution separations of steroids. The highest peak capacity could be achieved with the column packed with 1.5 microm totally porous particles. The columns packed with porous 1.7 microm and shell 2.7 microm particles showed very similar capacity. The monolith column provided the lowest capacity during gradient elution. The plate height (HETP) of the 2.7 microm Ascentis Express column was very similar to the HETP obtained with 1.5 microm and 1.7 microm totally porous particles. The Chromolith monolithic column displayed an efficiency that is comparable to that of columns packed with spherical particles having their diameter between 3 microm and 4 microm. A kinetic plot analysis is presented to compare the theoretical analysis speed of different separation media. At 200 bar, the monolith column provided the highest performance when the required plate number was higher than 5000 (N>5000), however the efficiency drifted off faster in the range of N<5000 than in the case of packed columns. If the possibility of maximum performance was utilized (1000 bar for sub-2 microm particles, 600 bar for shell particles and 200 bar for monolith column) the monolith column would provide the poorest efficiency, while the column, packed with 1.5 microm particles offered the shortest impedance time.
Fekete
Szabolcs
S
Formulation Development, Gedeon Richter Plc, Hungary, Budapest X., Gyömroi út 19-21, Hungary. fekete.szabolcs1@chello.hu
Fekete
Jeno
J
Ganzler
Katalin
K
D002626:Chemistry, Pharmaceutical / Q000295:instrumentation / Q000379:methods; D002845:Chromatography / Q000379:methods; D002853:Chromatography, Liquid / Q000295:instrumentation* / Q000379:methods*; D004867:Equipment Design; D004997:Ethinyl Estradiol / Q000032:analysis; D007700:Kinetics; D016912:Levonorgestrel / Q000032:analysis; D010316:Particle Size; D015203:Reproducibility of Results; D012984:Software; D013607:Tablets; D013696:Temperature
D013607:Tablets; D004997:Ethinyl Estradiol; D016912:Levonorgestrel