title: Cytogenotoxicity induced by PBDE-47 combined with PCB153 treatment in SH-SY5Y cells.
abstract: Polybrominated diphenyl ethers (PBDEs) and polychlorinated biphenyls (PCBs) are important recalcitrant halogenated compounds that have been regarded as major environmental pollutants. Recently, their concurrent appearance in the environment and humans and their structural and toxicological profile similarities have sparked interest in the potential toxicologic consequences of their coexposure. The aim of the current study was to evaluate the cytogenotoxic effects induced by 2,2',4,4'-tetrabromodiphenyl ether (PBDE-47) combined with 2,2',4,4',5,5'-hexachlorobiphenyl (PCB153) treatment in human neuroblastoma cells (SH-SY5Y) in vitro. SH-SY5Y cells were exposed to different concentrations of PBDE-47 (0, 2, 4, 8 μM) with or without PCB153 (5 μM) for 24 h. Thereafter, the cell viability, DNA damage, chromosomal abnormalities, and DNA-protein crosslinks (DPC) were determined. The results show that PBDE-47 and PCB153 alone and in combination induce DNA damage, with an increase in the frequency of micronuclei (MN) and DPC formation with increasing PBDE-47 concentration. In cells coexposed to PBDE-47 and PCB153, the cell viability significantly decreased while the MN frequency, DNA damage and DPC formation were all obviously increased compared to those of cells treated with the corresponding concentrations of PBDE-47 or PCB153 alone. Factorial analysis suggests that there were interactions between PBDE-47 and PCB153. The results imply that PBDE-47 interacts with PCB153 to inhibit cell viability and induce DNA damage, DPC formation, and chromosome abnormalities.
authors: Department of Environmental Health and MOE Key Laboratory of Environment and Health, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, Hubei, People's Republic of China.
authors: Weihong
authors: W
authors: He
authors: Aiguo
authors: A
authors: Wang
authors: Tao
authors: T
authors: Xia
authors: Ping
authors: P
authors: Gao
authors: Bayi
authors: B
authors: Xu
authors: Zhixia
authors: Z
authors: Xu
authors: Ping
authors: P
authors: He
authors: Xuemin
authors: X
authors: Chen
mesh_terms: D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D020552:Comet Assay; D004249:DNA Damage / Q000187:drug effects*; D004785:Environmental Pollutants / Q000633:toxicity*; D055768:Halogenated Diphenyl Ethers / Q000633:toxicity*; D006801:Humans; D015162:Micronucleus Tests; D011078:Polychlorinated Biphenyls / Q000633:toxicity*; D013778:Tetrazolium Salts / Q000032:analysis; D013844:Thiazoles / Q000032:analysis
chemical_list: D004785:Environmental Pollutants; D055768:Halogenated Diphenyl Ethers; D013778:Tetrazolium Salts; D013844:Thiazoles; C511295:2,2',4,4'-tetrabromodiphenyl ether; D011078:Polychlorinated Biphenyls; C022616:thiazolyl blue; C014024:2,4,5,2',4',5'-hexachlorobiphenyl