[Study on quality standard of Mucuna pruriens var. utilis].
OBJECTIVE
To provide scientific basis for the utilization and development of Mucuna pruriens var. utilis by establishing its quality control standard.


METHODS
The bioactive constituents were analyzed by TLC and HPLC. Moisture, ash and the extracts of Mucuna pruriens var. utilis were all determined.


RESULTS
The TLC spots of levodopa had similar color with the control group at the same position. The results of HPLC quantitative analysis showed that the linear range of levodopa was 26.45 to approximately 132.25 microg/mL, r = 0.9992, and the average recovery rate was 103.8%, RSD = 1.85%.


CONCLUSIONS
This method is convenient, accurate, reliable with good reproducibility, so it can be used to establish quality standard for the medicinal material.
Wu
Shi-Hong
SH
Guangxi Medical University, Nanning 530021, China. wushihong719@yahoo.com.cn
Jiang
Wei-Zhe
WZ
Lv
Li
L
Wu
Ling-Ling
LL
Lv
Cong
C
Shi
Xiao-Xia
XX
Su
Gui-Liang
GL
D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D004365:Drugs, Chinese Herbal / Q000737:chemistry* / Q000302:isolation & purification / Q000592:standards; D007980:Levodopa / Q000032:analysis* / Q000302:isolation & purification; D031299:Mucuna / Q000737:chemistry* / Q000254:growth & development; D010598:Pharmacognosy; D010946:Plants, Medicinal / Q000737:chemistry* / Q000254:growth & development; D011786:Quality Control; D015203:Reproducibility of Results; D012639:Seeds / Q000737:chemistry / Q000254:growth & development
D004365:Drugs, Chinese Herbal; D007980:Levodopa