Sustained NPY overexpression in the PVN results in obesity via temporarily increasing food intake.
Increasing neuropeptide Y (NPY) signaling in the paraventricular nucleus (PVN) by recombinant adeno-associated virus (rAAV)-mediated overexpression of NPY in rats, results in hyperphagia and obesity in rats. To determine the importance of hyperphagia in the observed obesity phenotype, we pair-fed a group of AAV-NPY-injected rats to AAV-control-injected rats and compared parameters of energy balance to ad libitum fed AAV-NPY-injected rats. For 3 weeks, AAV-NPY-injected rats, received the same amount of food as ad libitum-fed rats injected with control rAAV They did not gain more body weight than these controls. When allowed access to food ad libitum, these AAV-NPY-injected rats increased food intake, which subsequently decreased when rats reached the same body weight as AAV-NPY-injected rats that were fed ad libitum for the entire study. These data indicate that overexpression of NPY in the PVN results in obesity by increasing food intake until a certain body weight is achieved.
Tiesjema
Birgitte
B
Department of Neuroscience and Pharmacology, Rudolf Magnus Institute of Neuroscience, University Medical Center Utrecht, Utrecht, The Netherlands.
la Fleur
Susanne E
SE
Luijendijk
Mieneke C M
MC
Adan
Roger A H
RA
D000256:Adenoviridae; D000818:Animals; D001831:Body Temperature / Q000502:physiology; D001835:Body Weight / Q000502:physiology; D004195:Disease Models, Animal; D004435:Eating / Q000187:drug effects / Q000502:physiology; D006963:Hyperphagia / Q000150:complications* / Q000378:metabolism* / Q000503:physiopathology; D007267:Injections; D008297:Male; D009478:Neuropeptide Y / Q000008:administration & dosage / Q000378:metabolism* / Q000494:pharmacology; D009765:Obesity / Q000209:etiology* / Q000378:metabolism* / Q000503:physiopathology; D010286:Paraventricular Hypothalamic Nucleus / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar
D009478:Neuropeptide Y