title: Clinical factors such as B-type natriuretic peptide link to factor VII, endothelial NO synthase and estrogen receptor alpha polymorphism in elderly women.
abstract: OBJECTIVE
  This study evaluated the presence of genetic mutations in relation to thrombosis or atherosclerosis in elderly women.
  
  
  METHODS
  This is an observational study of 93 Japanese women with a mean age of 80.9 years recruited from outpatient clinics of Nagoya University and its related hospitals. Ten single nucleotide polymorphisms (SNPs) were studied. Each gene studied acts in or is related to either blood coagulation (factor V Leiden, prothrombin G20210A, factor XIII Val34Leu, factor VII Arg353Gln, MTHFR C677T, beta-fibrinogen G-455A, PAI-1 4G/5G), metabolic syndrome-related pathways (PPARalpha Leu162Val), or endothelium/estrogen system (eNOS Glu298Asp, ERalpha IVS1-401). SNPs were analyzed for their relation to clinical values including lipids, B-type natriuretic peptide (BNP), fasting plasma glucose, tumor necrosis factor-alpha, interleukin-6, cyclic GMP, and nitric oxide metabolites.
  
  
  RESULTS
  Comparisons between the distributions of different genotypes and clinical values showed three relationships. First, factor VII Arg353Gln and HDL-cholesterol (HDL-C) were linked to Arg/Arg carriers at higher levels (P=.049). The HDL-C to LDL-cholesterol ratio supported this link (P=.027). Second, eNOS Glu298Asp and triglycerides were linked to Glu/Glu carriers at higher levels (P=.031). Third, ERalpha IVS1-401 and BNP were related to CC genotype at lower levels (P=.031). Additionally, the last two relations showed that genotype does not influence the demarcation line of biomarkers, but the plasma/serum levels of biomarkers instead.
  
  
  CONCLUSIONS
  Correlations of factor VII Arg353Gln with HDL-C and eNOS Glu298Asp with triglycerides are new findings. Polymorphisms in the endothelium/estrogen system and the heart failure marker BNP are also correlated, with ERalpha IVS1-401 being the first identified marker. SNPs may be helpful for understanding the pathophysiology of atherosclerotic diseases in elderly women.
authors: Department of Geriatrics, Nagoya University Graduate School of Medicine, Tsuruma-cho 65, Showa-ku, Nagoya, Aichi 466-8550, Japan.
authors: Jun
authors: J
authors: Funami
authors: Toshio
authors: T
authors: Hayashi
authors: Hideki
authors: H
authors: Nomura
authors: Qun-Fang
authors: QF
authors: Ding
authors: Asako
authors: A
authors: Ishitsuka-Watanabe
authors: Hisako
authors: H
authors: Matsui-Hirai
authors: Koichiro
authors: K
authors: Ina
authors: Jie
authors: J
authors: Zhang
authors: Ze-Yun
authors: ZY
authors: Yu
authors: Akihisa
authors: A
authors: Iguchi
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D000375:Aging / Q000235:genetics*; D050197:Atherosclerosis / Q000097:blood / Q000235:genetics*; D004247:DNA / Q000235:genetics; D047628:Estrogen Receptor alpha / Q000235:genetics; D005167:Factor VII / Q000235:genetics; D005260:Female; D005838:Genotype; D006801:Humans; D007564:Japan; D008875:Middle Aged; D020097:Natriuretic Peptide, Brain / Q000097:blood*; D052250:Nitric Oxide Synthase Type III / Q000235:genetics; D020641:Polymorphism, Single Nucleotide; D013927:Thrombosis / Q000097:blood / Q000235:genetics*
chemical_list: D047628:Estrogen Receptor alpha; D020097:Natriuretic Peptide, Brain; D005167:Factor VII; D004247:DNA; C496314:NOS3 protein, human; D052250:Nitric Oxide Synthase Type III