title: Shifts in replication timing actively affect histone acetylation during nucleosome reassembly.
abstract: The entire genome is replicated in a programmed manner, with specific regions undergoing DNA synthesis at different times in S phase. Active genes generally replicate in early S phase, while repressed genes replicate late, and for some loci this process is developmentally regulated. Using a nuclear microinjection system, we demonstrate that DNA sequences originally packaged into nucleosomes containing deacetylated histones during late S become reassembled with acetylated histones after undergoing replication in early S. Conversely, a change from early to late replication timing is accompanied by repackaging into nucleosomes containing deacetylated histones. This is carried out by differential cell-cycle-controlled acetylation and deacetylation of histones H3 and H4. These studies provide strong evidence that switches in replication timing may play a role in the regulation of nucleosome structure during development.
authors: Department of Developmental Biology and Cancer Research, Institute for Medical Research Israel-Canada, Hebrew University Medical School, Ein Kerem, Jerusalem 91120, Israel.
authors: Laura
authors: L
authors: Lande-Diner
authors: Jianmin
authors: J
authors: Zhang
authors: Howard
authors: H
authors: Cedar
mesh_terms: D000107:Acetylation; D000818:Animals; D002460:Cell Line; D004261:DNA Replication / Q000502:physiology*; D006657:Histones / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D008845:Microinjections; D008957:Models, Genetic; D009707:Nucleosomes / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D011499:Protein Processing, Post-Translational; D051381:Rats; D016196:S Phase
chemical_list: D006657:Histones; D009707:Nucleosomes