title: Immunocytochemical detection of synaptophysin in C57BL/6 mice cochlea during aging process.
abstract: Aged mammals frequently exhibit a bilateral, progressive, and symmetric deafness related to the degeneration of auditory receptor. However, little is still known about aging effects on synapses in this receptor. Synaptophysin (Syp) is a 38 kDa Ca2+ binding glycoprotein widely found in presynaptic membrane and vesicles. The Syp has been found in presynaptic buttons of efferent auditory fibers, within the developing and adult auditory receptor. The detection of Syp in aged cochleae could provide relevant information about synaptic changes and receptor degeneration process observed in old animals. This paper focuses on aging linked changes related to the presence of Syp in cochleae of C57BL/6J mice (from 1 to 24 months old). Results showed that during the first months of age, no significant changes were observed in the Syp distribution under the basal pole of inner (IHCs) neither the outer (OHCs) hair cells. At six months of age, a significant decrease of Syp immunocytochemical detection appeared in fibers under the most external row of OHCs, but restricted to the cochlear basal coil. Only a very scarce reduction of Syp was noted under the IHC and the other OHC rows, also at the basal coil. From mice 9 months old on, a progressive decrease of the presence of Syp was found under IHC and all OHC rows starting at the basal coil and reaching the apical coil in the oldest mice. All these data could indicate that the cochlea aging process early affects to presynaptic membrane proteins of efferent endings fibers. This early alteration of cochleae efferent synapses could be involved in the whole degeneration of the Corti's organ.
authors: Department of Ophthalmology and Oto-Rhino-Laringology, Complutense University of Madrid, Apartado de Correos 60.075, 28080 Madrid, Spain.
authors: Maria Visitación
authors: MV
authors: Bartolome
authors: Pilar
authors: P
authors: Zuluaga
authors: Francisco
authors: F
authors: Carricondo
authors: Pablo
authors: P
authors: Gil-Loyzaga
mesh_terms: D000367:Age Factors; D000375:Aging / Q000502:physiology*; D000818:Animals; D003051:Cochlea / Q000166:cytology / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D016253:Microscopy, Immunoelectron / Q000379:methods; D009474:Neurons / Q000378:metabolism / Q000648:ultrastructure; D016708:Synaptophysin / Q000378:metabolism*
chemical_list: D016708:Synaptophysin