SAF-A/hnRNP-U localization in interphase and metaphase.
SAF-A/hnRNP U is an abundant nuclear protein that interacts specifically with nuclear matrix attachment region DNA (MAR) and RNA as a component of hnRNPs. SAF-A/hnRNP U was also shown to specifically bind mouse major satellite DNA (satMa). Antibodies against SAF-A and GFP-fusion constructs were used in the current work in order to trace SAF-A localization. In accordance with its diverse nucleic acid binding specificity, SAF-A was found to be localized in three different domains: outside the chromosomes, on the surface of the chromosome arms (probably MARs), and in the centromere region where it apparently binds specifically to the satMa. GFP-fusion constructs with different SAF-A/hnRNP U domains confirms the functional significance of the protein's functional domains in interphase cells. In telophase cells, the anti-SAF-A antibody signal appeared as a kind of network covering unfolded chromosomes.
Kukalev
A S
AS
Institute of Cytology, Russian Academy of Sciences, St. Petersburg, Russia.
Lobov
I B
IB
Percipalle
P
P
Podgornaya
O I
OI
D000818:Animals; D017209:Apoptosis; D019556:COS Cells; D002467:Cell Nucleus / Q000378:metabolism* / Q000648:ultrastructure; D002522:Chlorocebus aethiops; D033481:Chromosomes, Mammalian / Q000378:metabolism*; D034722:Heterogeneous-Nuclear Ribonucleoprotein U / Q000378:metabolism* / Q000648:ultrastructure; D007399:Interphase / Q000502:physiology; D008677:Metaphase / Q000502:physiology; D051379:Mice; D013692:Telophase / Q000502:physiology
D034722:Heterogeneous-Nuclear Ribonucleoprotein U