Determination of Prometryne in water and soil by HPLC-UV using cloud-point extraction.
A CPE-HPLC (UV) method has been developed for the determination of Prometryne. In this method, non-ionic surfactant Triton X-114 was first used to extract and pre-concentrate Prometryne from water and soil samples. The separation and determination of Prometryne were then carried out in an HPLC-UV system with isocratic elution using a detector set at 254 nm wavelength. The parameters and variables that affected the extraction were also investigated and the optimal conditions were found to be 0.5% of Triton X-114 (w/v), 3% of NaCl (w/v) and heat-assisted at 50 degrees C for 30 min. Using these conditions, the recovery rates of Prometryne ranged from 92.84% to 99.23% in water and 85.48% to 93.67% in soil, respectively, with all the relative standard deviations less than 3.05%. Limit of detection (LOD) and limit of quantification (LOQ) were 3.5 microg L(-1) and 11.0 microg L(-1) in water and 4.0 microg kg(-1) and 13.0 microg kg(-1) in soil, respectively. Thus, we developed a method that has proven to be an efficient, green, rapid and inexpensive approach for extraction and determination of Prometryne from soil samples.
Zhou
Jihai
J
College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing, Jiangsu Province 210095, China.
Chen
Jiandong
J
Cheng
Yanhong
Y
Li
Daming
D
Hu
Feng
F
Li
Huixin
H
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D055772:Green Chemistry Technology; D006540:Herbicides / Q000032:analysis; D006358:Hot Temperature; D017830:Octoxynol; D011092:Polyethylene Glycols; D011400:Prometryne / Q000032:analysis / Q000302:isolation & purification*; D012989:Soil Pollutants / Q000032:analysis / Q000302:isolation & purification*; D014874:Water Pollutants, Chemical / Q000032:analysis / Q000302:isolation & purification*
D006540:Herbicides; D012989:Soil Pollutants; D014874:Water Pollutants, Chemical; D011400:Prometryne; D011092:Polyethylene Glycols; D017830:Octoxynol; C010615:Nonidet P-40