Quantitative phase-gradient imaging at high resolution with asymmetric illumination-based differential phase contrast.
We describe a full-field phase-gradient imaging method: asymmetric illumination-based differential phase contrast (AIDPC). Imaging properties of AIDPC are evaluated using the phase-gradient transfer-function approach and elucidated with experimental images of an optical fiber and a histochemical preparation of a skeletal muscle section. In comparison with full-field differential interference contrast, AIDPC does not require phase shifting for quantitative imaging of phase gradient, provides artifact-free images of birefringent specimens, requires shorter camera exposure, and has larger depth of focus. It is amenable to transfer-function engineering, simultaneous fluorescence imaging, and automated live cell imaging.
Mehta
Shalin B
SB
Optical Bioimaging Lab, National University of Singapore, Graduate School for Integrative Sciences and Engineering, Department of Biological Sciences, Singapore. shalin@nus.edu.sg
Sheppard
Colin J R
CJ
D000042:Absorption; D008029:Lighting / Q000295:instrumentation / Q000379:methods*; D008858:Microscopy, Phase-Contrast / Q000295:instrumentation / Q000379:methods*; D018482:Muscle, Skeletal / Q000378:metabolism; D055100:Optical Fibers