title: [Interference of osteopontin expression inhibits the invasion and metastasis of human hepatocellular carcinoma cell lines].
abstract: OBJECTIVE
  To investigate the effect of osteopontin (OPN) on the invasion and metastasis of human hapatocellular carcinoma (HCC).
  
  
  METHODS
  HCC cell lines (HCC-LM3) were transfected with the chemically synthesized small interfering RNA (siRNA). Real-time PCR and Western blot were used to quantify the mRNA and OPN protein levels. The malignant phenotypes including cellular growth, colony formation and invasion capability of the HCC cells were analyzed.
  
  
  RESULTS
  The OPN mRNA and proteins levels were decreased by 75% and 80% in OPN siRNA treated cells. Colony formation and migratory capability were reduced in OPN siRNA treated cells (P < 0.05).
  
  
  CONCLUSIONS
  The specific siRNA is able to reduce the OPN expression at both the mRNA and protein levels and significantly inhibits the invasiveness of HCC cells.
authors: Department of Hepatobilary Surgery and Gastroenterology, First Municipal People's Hospital of Guangzhou, Guangzhou Medical College, Guagnzhou, China.
authors: Fan
authors: F
authors: Lin
authors: Yu-yuan
authors: YY
authors: Li
authors: Jin-tang
authors: JT
authors: Xia
authors: Min-jie
authors: MJ
authors: Wen
authors: Yue-yuan
authors: YY
authors: Lai
authors: Wen-song
authors: WS
authors: Cai
authors: Zhao-feng
authors: ZF
authors: Wu
authors: Shao-feng
authors: SF
authors: Fan
mesh_terms: D006528:Carcinoma, Hepatocellular / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D015972:Gene Expression Regulation, Neoplastic; D005822:Genetic Vectors; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D009361:Neoplasm Invasiveness / Q000517:prevention & control; D009362:Neoplasm Metastasis / Q000517:prevention & control; D053495:Osteopontin / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D034741:RNA, Small Interfering / Q000235:genetics*; D014162:Transfection
chemical_list: D012333:RNA, Messenger; D034741:RNA, Small Interfering; D053495:Osteopontin