Current trends, biological foundations and future prospects of oocyte and embryo cryopreservation.
For a long time, the cryopreservation of gametes and embryos remained a major hurdle for the clinicians and scientists in terms of success. However, recent technical advancement in the field of cryobiology has opened up various options for freezing gametes and embryos at different developmental stages. The tendency of the IVF world to switch over to natural-cycle IVF and to elective single-embryo transfer has put cryotechnology in the forefront of research. Still, the intricacies of the cold-induced changes in human gametes and embryos that could affect the intracellular and developmental processes need to be known. The transcriptomics, proteomics and metabolomic platforms hold promise for elucidating these complex processes during cryopreservation processes.
Varghese
Alex C
AC
Center for Reproductive Medicine, Cleveland Clinic, Cleveland, Ohio, USA.
Nagy
Zsolt Peter
ZP
Agarwal
Ashok
A
D021301:Antifreeze Proteins / Q000378:metabolism; D000975:Antioxidants / Q000378:metabolism; D050260:Carbohydrate Metabolism; D002470:Cell Survival; D015925:Cryopreservation; D004622:Embryo, Mammalian; D005260:Female; D005307:Fertilization in Vitro; D006801:Humans; D009865:Oocytes; D011247:Pregnancy; D011256:Pregnancy Outcome
D021301:Antifreeze Proteins; D000975:Antioxidants