Hemoglobin conjugated micelles based on triblock biodegradable polymers as artificial oxygen carriers.
An artificial oxygen carrier is constructed by conjugating hemoglobin molecules to biodegradable micelles. Firstly a series of triblock copolymers (PEG-PMPC-PLA) in which the middle block contains pendant propargyl groups were synthesized and characterized. After the amphiphilic copolymer was self-assembled into core-shell micelles in aqueous solution, azidized hemoglobin molecules protected by carbon monoxide (CO) were conjugated to the micelles via click reaction between the propargyl and azido groups. The conjugation causes an increase of the micelle's mean diameter. Maximum conjugation ratio is 250 wt% in the hemoglobin-conjugated micelles (HCMs). Oxygen-binding ability of the HCMs was demonstrated by converting the CO-binding state of the HCMs into O(2)-binding state.
Shi
Quan
Q
State Key Laboratory of Polymer Physics and Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, People's Republic of China.
Huang
Yubin
Y
Chen
Xuesi
X
Wu
Meng
M
Sun
Jing
J
Jing
Xiabin
X
D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry; D001802:Blood Substitutes / Q000737:chemistry* / Q000378:metabolism*; D002248:Carbon Monoxide / Q000737:chemistry / Q000378:metabolism; D002417:Cattle; D008823:Micelles; D010100:Oxygen / Q000378:metabolism; D011092:Polyethylene Glycols / Q000737:chemistry; D011108:Polymers / Q000737:chemistry*
D001672:Biocompatible Materials; D001802:Blood Substitutes; D008823:Micelles; D011108:Polymers; C542623:monomethoxypolyethyleneglycol-polylactide block copolymer; D011092:Polyethylene Glycols; D002248:Carbon Monoxide; D010100:Oxygen