Protein profile of the luteal phase endometrium by tissue microarray assessment.
To investigate the luteal phase endometrial expression of leukemia inhibitor factor (LIF), insulin-like growth factor 1 (IGF-1), progesterone receptor (PR), claudin 4 (CLDN4), vascular-endothelial growth factor receptor 3 (VEGFR-3), bone morphogenetic protein 4 (BMP-4) and citokeratin 7 (CK-7), we obtained luteal phase endometrial samples from 52 women. Samples were dated and integrated using a tissue microarray (TMA). Samples were immunostained for LIF, IGF-1, PR, CLDN4, VEGFR-3, BMP-4 and CK-7. Frequencies of positive expressions at the early, mid and late luteal phases were compared by two proportions test. Concomitant expression of these proteins was assessed with Chi-square or Fischer's test. The frequency of LIF was positively correlated to the frequency of IGF-1 (r = 0.99; p < 0.05) and PR (r = 0.99; p < 0.05), and the correlation between IGF-1 and PR tended to be significant (r = 0.98; p < 0.1). The expression of PR was associated with the absence of CLDN4 (p < 0.001). Thus, expression of LIF, IGF-1 and PR are correlated during the luteal phase, and immunohistochemistry for these proteins might be used to assist in the assessment of endometrial maturation. In addition, the expression of CLDN4 and PR was not concomitant, warranting further investigation on the relationship of their endometrial expression.
Serafini
Paulo
P
Department of Gynecology, Faculty of Medicine, University of São Paulo, São Paulo, Brazil. pserafini@huntington.com.br
Da Rocha
André Monteiro
AM
De Toledo Osório
Cyntia Aparecida Bueno
CA
Smith
Gary Daniel
GD
Hassun
Pericles Assad
PA
da Silva
Ismael Guerreiro Dale Cotrim Guerreiro
IG
Da Motta
Eduardo Leme Alves
EL
Baracat
Edmund Chada
EC
D000328:Adult; D055415:Bone Morphogenetic Protein 4 / Q000378:metabolism; D016009:Chi-Square Distribution; D062506:Claudin-4; D004717:Endometrium / Q000378:metabolism*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D007334:Insulin-Like Growth Factor I / Q000378:metabolism; D053552:Keratin-7 / Q000378:metabolism; D053605:Leukemia Inhibitory Factor / Q000378:metabolism; D008183:Luteal Phase / Q000378:metabolism*; D008565:Membrane Proteins / Q000378:metabolism; D018579:Patient Selection; D040081:Protein Array Analysis; D011980:Receptors, Progesterone / Q000378:metabolism; D040321:Vascular Endothelial Growth Factor Receptor-3 / Q000378:metabolism
D055415:Bone Morphogenetic Protein 4; C568157:CLDN4 protein, human; D062506:Claudin-4; D053552:Keratin-7; D053605:Leukemia Inhibitory Factor; D008565:Membrane Proteins; D011980:Receptors, Progesterone; D007334:Insulin-Like Growth Factor I; D040321:Vascular Endothelial Growth Factor Receptor-3