The effect of collagen-binding NGF-beta on the promotion of sciatic nerve regeneration in a rat sciatic nerve crush injury model.
Nerve growth factor plays a critical role in peripheral nerve regeneration. However, the lack of efficient NGF delivery approach limits its clinical application. It has demonstrated in our previous work that the native human NGF-beta (NAT-NGF) fused with a collagen-binding domain (CBD) could bind to collagen specifically. Since collagen is the major component of nerve extracellular matrix, we speculated that the collagen-binding NGF would target to nerve cells and improve their regeneration. In this report, we found that the fusion protein could specifically bind to endogenous collagen of the rat sciatic nerves and maintain NGF activity both in vitro and in vivo. In the rat sciatic nerve crush injury model, we found that collagen-binding NGF could be retained and concentrated at the nerve injured site to promote nerve repair and enhance function recovery following nerve damage. Thus, the collagen-binding NGF could improve the repair of peripheral nerve injury.
Sun
Wenjie
W
Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, 3 Nanyitiao, Zhongguancun, Beijing 100080, China.
Sun
Changkai
C
Lin
Hang
H
Zhao
Hui
H
Wang
Jingyu
J
Ma
Hui
H
Chen
Bing
B
Xiao
Zhifeng
Z
Dai
Jianwu
J
D000818:Animals; D001681:Biological Assay; D003094:Collagen / Q000378:metabolism*; D003692:Delayed-Action Preparations; D004195:Disease Models, Animal; D055724:Electrophysiological Phenomena; D006801:Humans; D009186:Myelin Sheath / Q000378:metabolism; D009409:Nerve Crush; D020932:Nerve Growth Factor / Q000378:metabolism*; D009416:Nerve Regeneration; D011485:Protein Binding; D051381:Rats; D012584:Sciatic Nerve / Q000293:injuries* / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology
D003692:Delayed-Action Preparations; D003094:Collagen; D020932:Nerve Growth Factor