Multivalent binding of carbohydrates by the human alpha-defensin, HD5.
Four of the six human alpha-defensins (human neutrophil peptides 1-3 and human alpha-defensin 5; HD5) have a lectin-like ability to bind glycosylated proteins. Using HD5 as a model, we applied surface plasmon resonance techniques to gain insights into this property. HD5 bound natural glycoproteins > neoglycoproteins based on BSA > nonglycosylated BSA >> free sugars. The affinity of HD5 for simple sugars covalently bound to BSA was orders of magnitude greater than its affinity for the same sugars in solution. The affinity of HD5 for protein-bound carbohydrates resulted from multivalent interactions which may also involve noncarbohydrate residues of the proteins. HD5 showed concentration-dependent self-association that began at submicromolar concentrations and proceeded to dimer and tetramer formation at concentrations below 5 microM. The (R9A, R28A) and (R13A, R32A) analogs of HD5 showed greatly reduced self-association as well as minimal binding to BSA and to BSA-affixed sugars. From this and other evidence, we conclude that the extensive binding of HD5 to (neo)glycoproteins results from multivalent nonspecific interactions of individual HD5 molecules with carbohydrate and noncarbohydrate moieties of the target molecule and that the primary binding events are magnified and enhanced by subsequent in situ assembly and oligomerization of HD5. Self-association and multivalent binding may play integral roles in the ability of HD5 to protect against infections caused by viruses and other infectious agents.
Lehrer
Robert I
RI
David Geffen School of Medicine at University of California at Los Angeles, 90095, USA. rlehrer@mednet.ucla.edu
Jung
Grace
G
Ruchala
Piotr
P
Andre
Sabine
S
Gabius
Hans J
HJ
Lu
Wuyuan
W
D000117:Acetylglucosamine / Q000378:metabolism; D000998:Antiviral Agents / Q000378:metabolism*; D050260:Carbohydrate Metabolism / Q000276:immunology*; D002460:Cell Line; D004306:Dose-Response Relationship, Immunologic; D006031:Glycosylation; D015699:HIV Envelope Protein gp120 / Q000378:metabolism; D015497:HIV-1 / Q000276:immunology / Q000378:metabolism; D006389:Hemagglutinins, Viral / Q000378:metabolism; D018259:Herpesvirus 1, Human / Q000276:immunology / Q000378:metabolism; D006801:Humans; D011485:Protein Binding / Q000276:immunology; D012710:Serum Albumin, Bovine / Q000378:metabolism; D014759:Viral Envelope Proteins / Q000378:metabolism; D023084:alpha-Defensins / Q000378:metabolism*
D000998:Antiviral Agents; C500225:DEFA5 protein, human; D015699:HIV Envelope Protein gp120; D006389:Hemagglutinins, Viral; D014759:Viral Envelope Proteins; D023084:alpha-Defensins; C039447:glycoprotein D, Human herpesvirus 1; D012710:Serum Albumin, Bovine; D000117:Acetylglucosamine