title: The gene bap, involved in biofilm production, is present in Staphylococcus spp. strains from nosocomial infections.
abstract: This study analyzed ten strains of coagulase-negative staphylococci (CNS) involved in nosocomial infections in three Brazilian hospitals. Their antibiotic susceptibility profile showed that most strains exhibited multiple antibiotic resistance and possessed the mecA gene. The ability of these strains to adhere to polystyrene microtiter plates was also tested and nine of them proved to be biofilm producers at least in one of the three conditions tested: growth in TSB, in TSB supplemented with NaCl, or in TSB supplemented with glucose. The presence of the bap gene, which codes for the biofilm-associated protein (Bap), was investigated in all ten strains by PCR. AU strains were bop-positive and DNA sequencing experiments confirmed that the fragments amplified were indeed part of a bap gene. The presence of the icaA gene, one of the genes involved in polysaccharide intercellular adhesin (PIA) formation, was also detected by PCR in eight of the ten strains tested. The two icaA-negative strains were either weak biofilm producer or no biofilm producer, although they were bop-positive. To our knowledge, this is the first report demonstrating the presence of the bap gene in nosocomial isolates of CNS, being also the first report on the presence of this gene in Staphylococcus haemolyticus and S. cohnii.
authors: Instituto de Microbiologia Prof. Paulo de Góes, Universidade Federal do Rio de Janeiro, Rio de Janeiro 21941-590, Brasil.
authors: Amina
authors: A
authors: Potter
authors: Hilana
authors: H
authors: Ceotto
authors: Marcia
authors: M
authors: Giambiagi-Demarval
authors: Kátia Regina Netto
authors: KR
authors: dos Santos
authors: Ingolf F
authors: IF
authors: Nes
authors: Maria do Carmo de Freire
authors: Mdo C
authors: Bastos
mesh_terms: D001422:Bacterial Adhesion; D001426:Bacterial Proteins / Q000235:genetics*; D018441:Biofilms / Q000254:growth & development; D001938:Brazil; D003428:Cross Infection / Q000382:microbiology*; D004269:DNA, Bacterial / Q000235:genetics; D024901:Drug Resistance, Multiple, Bacterial; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods; D017422:Sequence Analysis, DNA; D013203:Staphylococcal Infections / Q000382:microbiology*; D013210:Staphylococcus / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification* / Q000502:physiology
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial