title: N-glycosylation status of beta-haptoglobin in sera of patients with colon cancer, chronic inflammatory diseases and normal subjects.
abstract: N-glycosylation status of purified beta-haptoglobin from sera of 17 patients, and from sera of 14 healthy volunteer subjects, was compared by blotting with various lectins and antibodies. Patients in this study were diagnosed as having colon cancer through histological examination of each tumor tissue by biopsy. Blotting index of serum beta-haptoglobin with Aleuria aurantia lectin (AAL) was clearly higher for cancer patients than for healthy subjects. No such distinction was observed for blotting with three other lectins and two monoclonal antibodies. To determine tumor-associated reactivity of AAL binding as compared to inflammatory processes in colonic tissues, beta-haptoglobin separated from sera of 5 patients with Crohn's disease (CD), and 4 patients with ulcerative colitis (UC), was studied. All these cases, except one case of UC, showed AAL index lower than that in cancer cases, similarly to healthy subjects. The higher AAL binding of beta-haptoglobin in colon cancer patients than in healthy subjects appeared to be due to alpha-L-fucosyl residue, since it was eliminated by bovine kidney alpha-fucosidase treatment. N-linked glycans of serum haptoglobin from colon cancer patients vs. healthy subjects were released by N-glycanase, fluorescence-labeled, and subjected to normal-phase high performance liquid chromatography (NP-HPLC). Glycan structures were determined based on glucose unit (GU) values and their changes upon sequential treatment with various exoglycosidases. Glycosyl sequences and their branching status of glycans from 14 cases of serum beta-haptoglobin were characterized. The identified glycans were sialylated or nonsialylated, bi-antennary or tri-antennary structures, with or without terminal fucosylation.
authors: Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Daejeon, Republic of Korea. hakomori@u.washington.edu
authors: Seung-Yeol
authors: SY
authors: Park
authors: Seon-Joo
authors: SJ
authors: Yoon
authors: Yeon-Tae
authors: YT
authors: Jeong
authors: Jin-Man
authors: JM
authors: Kim
authors: Ji-Yeon
authors: JY
authors: Kim
authors: Bradford
authors: B
authors: Bernert
authors: Thomas
authors: T
authors: Ullman
authors: Steven H
authors: SH
authors: Itzkowitz
authors: Jung-Hoe
authors: JH
authors: Kim
authors: Sen-itiroh
authors: SI
authors: Hakomori
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D016022:Case-Control Studies; D045744:Cell Line, Tumor; D002908:Chronic Disease; D003110:Colonic Neoplasms / Q000097:blood* / Q000473:pathology; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006031:Glycosylation; D006242:Haptoglobins / Q000378:metabolism*; D006801:Humans; D015212:Inflammatory Bowel Diseases / Q000097:blood*; D008297:Male; D008875:Middle Aged
chemical_list: D006242:Haptoglobins