Analysis of intestinal injuries induced by ricin in vitro using SPR technology and MS identification.
The present study found that ricin toxicity did not only manifest itself as inhibition of protein synthesis, but also induced apoptosis of immune cells and played an extremely significant role in intestinal injury. In this report, we describe a novel method to estimate binding events occurring on intestinal brush border membranes (BBM) based on SPR technology in an attempt to mimic the real intestinal surface capable of interacting physically and/or actively with certain biological molecules. Combined with HPCE-ESI-MS indentification, we obtained 28 kinds of proteins in BBM that interacted with ricin.
Liu
Linna
L
Institute of Military Veterinary, Academy of Military Medical Science, Changchun 130062, China.|Department of Theoretic Veterinary Medicine, Colledge of Animal Science and Veterinary, Jilin University, Changchun 130062, China.
Gao
Hongwei
H
Institute of Military Veterinary, Academy of Military Medical Science, Changchun 130062, China.
Li
Jiping
J
Institute of Military Veterinary, Academy of Military Medical Science, Changchun 130062, China.
Dong
Ying
Y
Institute of Military Veterinary, Academy of Military Medical Science, Changchun 130062, China.
Liu
Ning
N
Institute of Military Veterinary, Academy of Military Medical Science, Changchun 130062, China.
Wan
Jiayu
J
Institute of Military Veterinary, Academy of Military Medical Science, Changchun 130062, China.
Liu
Wensen
W
Institute of Military Veterinary, Academy of Military Medical Science, Changchun 130062, China.
Sun
Yucheng
Y
Institute of Military Veterinary, Academy of Military Medical Science, Changchun 130062, China.
Xu
Ming
M
Institute of Military Veterinary, Academy of Military Medical Science, Changchun 130062, China.
D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D015374:Biosensing Techniques; D007413:Intestinal Mucosa / Q000187:drug effects*; D007422:Intestines / Q000187:drug effects / Q000293:injuries*; D013058:Mass Spectrometry; D008567:Membranes, Artificial; D051379:Mice; D008871:Microvilli / Q000187:drug effects* / Q000378:metabolism; D014176:Protein Biosynthesis / Q000187:drug effects; D051381:Rats; D017207:Rats, Sprague-Dawley; D012276:Ricin / Q000378:metabolism / Q000633:toxicity*; D020349:Surface Plasmon Resonance
D008567:Membranes, Artificial; D012276:Ricin