Regulation of preprosomatostatin 1 (PSS1) gene expression by 17beta-estradiol and identification of the PSS1 promoter region in orange-spotted grouper (Epinephelus coioides).
In the present paper the effects of 17beta-estradiol on the expression of the preprosomatostatin 1 (PSS1) in the orange-spotted grouper hypothalamus and ovary were investigated. Results from in vivo of intraperitoneal injection and in vitro static cultures showed that estradiol increased the mRNA expression of PSS1 gene in both hypothalamus and ovary. To investigate the molecular basis of the estrogen regulation on PSS1 gene expression, we cloned the upstream region of 848bp from the translation initiation codon of the grouper PSS1 gene. The TATA-box and putative transcription factor binding sites were identified using computer analysis. Transient transfections with promoter-luciferase reporter constructs together with hER expression vector were carried out in MCF-7 cell line. The results suggest that the region from -848 to -373bp, containing five putative ERE half sites, may contribute to the promoter activity induced by estradiol. These results represent the first demonstration at the molecular level of the regulation of PSS1 gene by 17beta-estradiol in fish.
Zhang
Li
L
State Key Laboratory of Biocontrol, Institute of Aquatic Economic Animals, School of Life Sciences, Sun Yat-Sen University, Guangzhou 510275, PR China.
Li
Wensheng
W
Hong
Xun
X
Lin
Haoran
H
D024506:5' Flanking Region / Q000235:genetics; D000818:Animals; D001483:Base Sequence; D045744:Cell Line, Tumor; D003001:Cloning, Molecular; D004958:Estradiol / Q000494:pharmacology*; D004967:Estrogens / Q000494:pharmacology; D005260:Female; D005399:Fishes / Q000235:genetics*; D005786:Gene Expression Regulation / Q000187:drug effects*; D006801:Humans; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D017422:Sequence Analysis, DNA; D013004:Somatostatin / Q000235:genetics*
D004967:Estrogens; D012333:RNA, Messenger; D004958:Estradiol; D013004:Somatostatin