Applications of nucleic acid chaperone activity of CspA and its homologues.
In Escherichia coli, the cold shock response is exerted upon temperature change from 37 to 15 degrees C and is characterized by induction of several cold shock proteins including its major cold shock protein, CspA. E. coli CspA family consists of nine members, CspA to CspI. CspA and some of its homologues play a critical role in cold acclimation of cells as RNA chaperones by destabilizing secondary structures in RNAs. Here, we showed that the nucleic acid melting activity of Csp proteins can be used to facilitate reactions, such as RT-PCR or RNA cleavage reactions by endoribonucleases, which are hindered by presence of secondary structures in the DNA/RNA substrate used. The low substrate specificity of Csps together with their compatibility with various enzymes and their stability and activity over a broad temperature range makes them ideal candidates to be used for a variety of processes.
Phadtare
Sangita
S
Department of Biochemistry, Robert Wood Johnson Medical School, Piscataway, NJ 08854, USA. phadtasa@umdnj.edu
Zhu
Ling
L
Uemori
Takashi
T
Mukai
Hiroyuki
H
Kato
Ikunoshin
I
Inouye
Masayori
M
D057773:Cold Shock Proteins and Peptides; D003080:Cold Temperature; D004722:Endoribonucleases / Q000378:metabolism; D004926:Escherichia coli / Q000502:physiology*; D029968:Escherichia coli Proteins / Q000378:metabolism*; D006360:Heat-Shock Proteins / Q000378:metabolism*; D006801:Humans; D018832:Molecular Chaperones / Q000378:metabolism*; D009696:Nucleic Acids / Q000378:metabolism*; D012194:RNA-Directed DNA Polymerase / Q000378:metabolism; D013312:Stress, Physiological; D013379:Substrate Specificity
D057773:Cold Shock Proteins and Peptides; C498192:CspA protein, E coli; D029968:Escherichia coli Proteins; D006360:Heat-Shock Proteins; D018832:Molecular Chaperones; D009696:Nucleic Acids; D012194:RNA-Directed DNA Polymerase; D004722:Endoribonucleases