Characterization of the replication-competent porcine endogenous  retrovirus class B molecular clone originated from Korean  domestic pig.
Xenotransplantation from pigs offers an opportunity to resolve the shortage of human organs. The porcine endogenous retrovirus (PERV) cannot be eliminated because of its presence in the germline DNA. Three subgroups of the replication-competent PERV (PERV-A, PERV-B, and PERV-C) have been identified in pigs. We constructed a molecular clone of PERV-B from a Korean domestic pig BAC clone containing PERV genomes, and its replication competency was characterized in human cells. The pol region of PERV-B was detected in the genomic DNA of 293T cells transfected with PERV-B (465D1) and in the genomic DNA of 293T cells infected with PERV-B (465D1) viruses. The 293T cells transfected with PERV-B (465D1) were maintained for 140 days. PERV-B (465D1) showed the stationary replication competence with no toxicity in the cell growth.
Kim
Na Young
NY
Department of Life Science, Sogang University, Seoul 121-742, South Korea.
Lee
Donghee
D
Lee
Jungeun
J
Park
Eung Woo
EW
Jung
Woon-Won
WW
Yang
Jai Myung
JM
Kim
Young Bong
YB
D000818:Animals; D002460:Cell Line; D002470:Cell Survival; D022202:Chromosomes, Artificial, Bacterial; D016000:Cluster Analysis; D020077:Endogenous Retroviruses / Q000235:genetics* / Q000302:isolation & purification* / Q000472:pathogenicity; D006801:Humans; D007723:Korea; D008969:Molecular Sequence Data; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D034421:Sus scrofa / Q000821:virology*; D014779:Virus Replication