title: Plasmid-based system for high-level gene expression and antisense gene knockdown in Bartonella henselae.
abstract: Six broad-host-range plasmid vectors were developed to study gene expression in Bartonella henselae. The vectors were used to express a beta-galactosidase reporter gene in B. henselae and to generate antisense RNA for gene knockdown. When applied to ompR, a putative transcription response regulator of B. henselae, this antisense RNA gene knockdown strategy reduced bacterial invasion of human endothelial cells by over 60%.
authors: Department of Molecular Medicine, College of Medicine, MDC7, University of South Florida, Tampa, FL 33612, USA.
authors: Devin
authors: D
authors: Gillaspie
authors: Izabella
authors: I
authors: Perkins
authors: Kellie
authors: K
authors: Larsen
authors: Amy
authors: A
authors: McCord
authors: Scott
authors: S
authors: Pangonis
authors: Debra
authors: D
authors: Sweger
authors: Mohamed N
authors: MN
authors: Seleem
authors: Nammalwar
authors: N
authors: Sriranganathan
authors: Burt E
authors: BE
authors: Anderson
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D018416:Bartonella henselae / Q000235:genetics / Q000378:metabolism / Q000472:pathogenicity*; D042783:Endothelial Cells / Q000382:microbiology*; D015964:Gene Expression Regulation, Bacterial; D055785:Gene Knockdown Techniques; D017930:Genes, Reporter; D005822:Genetic Vectors; D006801:Humans; D010957:Plasmids / Q000235:genetics*; D016372:RNA, Antisense; D001616:beta-Galactosidase / Q000235:genetics / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D016372:RNA, Antisense; D001616:beta-Galactosidase