[Protection effects of acetylcholinesterase inhibitors on apoptosis].
OBJECTIVE
To investigate whether tacrine and donepezil can prevent apoptosis induced by Lipopolysaccharides.


METHODS
Phase-contrast microscopes was used to observe the morphological changes of Vero cells. Cell counting kit-8 was used to measure cell survival vitro. DNA fragment was analyzed by agarose gel electrophoresis to determine the apoptotic biochemical changes.


RESULTS
Vero cells treated with 400 microg/ml and 500 microg/ml Lipopolysaccharides exhibited cell apoptosis. 10 micromol/L tacrine provided protective effect to 500 microg/ml Lipopolysaccharides induced cell apoptosis measured by Phase-contrast microscopes, cell counting kit-8 and DNA fragment analyze. However, donepezil did not show any protective effect of the apoptosis induced by 500 microg/ml Lipopolysaccharides.


CONCLUSIONS
Lipopolysaccharides can induce apoptosis in Vero cells to built an apoptotic model in vitro. Tacrine rather than donepezil can inhibit Lipopolysaccharides induced apoptosis in Vero cells.
Huang
Jing
J
Beliing An Ding Hospital, Capital Medical University, Beijing 100088, China.
Yi
Yao
Y
Bi
Sheng-li
SL
Chen
Si-yong
SY
Wang
Chuan-yue
CY
D000818:Animals; D017209:Apoptosis / Q000187:drug effects* / Q000502:physiology; D002452:Cell Count / Q000706:statistics & numerical data; D049109:Cell Proliferation / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects*; D002522:Chlorocebus aethiops; D002800:Cholinesterase Inhibitors / Q000494:pharmacology*; D000077265:Donepezil; D004336:Drug Antagonism; D006367:HeLa Cells / Q000187:drug effects / Q000473:pathology; D006509:Hepatitis B / Q000473:pathology*; D006801:Humans; D007189:Indans / Q000494:pharmacology; D008070:Lipopolysaccharides / Q000037:antagonists & inhibitors / Q000633:toxicity; D010880:Piperidines / Q000494:pharmacology; D013619:Tacrine / Q000494:pharmacology; D014709:Vero Cells
D002800:Cholinesterase Inhibitors; D007189:Indans; D008070:Lipopolysaccharides; D010880:Piperidines; D013619:Tacrine; D000077265:Donepezil