title: Cutting edge: B220+CCR9- dendritic cells are not plasmacytoid dendritic cells but are precursors of conventional dendritic cells.
abstract: Mouse lymphoid organs contain two major subsets of dendritic cells (DC) that differ in their phenotype and functions: conventional DC (cDC) and plasmacytoid DC (pDC). Recently, it has been proposed that differential expression of CCR9 could distinguish functionally distinct pDC subsets. We show that B220(+)CCR9(-) DC do not express classical pDC markers and have a developmental origin different from that of pDC. Furthermore, B220(+)CCR9(-) DC do not secrete IFN-alpha in response to CpG and, unlike pDC, can efficiently present exogenous Ags. Our results demonstrate that B220(+)CCR9(-) DC do not represent a subset of pDC. After in vivo transfer, these cells down-regulate B220 expression and convert into the two major cDC subsets, showing that they are a developmental stage of cDC differentiation.
authors: Immunology Division, Walter and Eliza Hall Institute of Medical Research, Parkville, Victoria, Australia. segura@wehi.edu.au
authors: Elodie
authors: E
authors: Segura
authors: June
authors: J
authors: Wong
authors: José A
authors: JA
authors: Villadangos
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D019070:Cell Lineage; D017690:Cell Transplantation; D003713:Dendritic Cells / Q000145:classification / Q000166:cytology* / Q000637:transplantation; D016130:Immunophenotyping; D017493:Leukocyte Common Antigens; D051379:Mice; D054388:Receptors, CCR
chemical_list: C119309:CC chemokine receptor 9; D054388:Receptors, CCR; D017493:Leukocyte Common Antigens