title: Genetic variation and drought response in two Populus x euramericana genotypes through 2-DE proteomic analysis of leaves from field and glasshouse cultivated plants.
abstract: Genotype and water deficit effects on leaf 2-DE protein profiles of two Populus deltoides x Populus nigra, cv. 'Agathe_F' and 'Cima', were analysed over a short-term period of 18 days in glasshouse using 4-month-old rooted cuttings and over a long-lasting period of 86 days in open field using 4-year-old rooted cuttings. Leaf proteomes were analyzed using two-dimensional gel electrophoresis, and proteins were identified after database searching from MS peptide spectra. A reliable genotype effect was observed in the leaf proteome over experiment locations, water regimes and sampling dates. Quantitative differences between genotypes were found. Most of them corresponded to proteins matching isoforms or post-translational modification variants. However, 'Cima' displayed the highest abundance of antioxidant enzymes. In response to water deficit, about 10% of the reproducible spots significantly varied regardless of the experiment location, among which about 25% also displayed genotype-dependent variations. As a whole, while 'Cima' differed from 'Agathe_F' by increased abundance of enzymes involved in photorespiration and in oxidative stress, 'Agathe_F' was mainly differentiated by increased abundance of enzymes involved in photosynthesis.
authors: Université d'Orléans, UFR-Faculté des Sciences, Laboratoire de Biologie des Ligneux et des Grandes Cultures, UPRES EA 1207, rue de Chartres, BP 6759, F-45067 Orléans Cedex 02, France.
authors: Ludovic
authors: L
authors: Bonhomme
authors: Romain
authors: R
authors: Monclus
authors: Delphine
authors: D
authors: Vincent
authors: Sabine
authors: S
authors: Carpin
authors: Stéphane
authors: S
authors: Claverol
authors: Anne-Marie
authors: AM
authors: Lomenech
authors: Valérie
authors: V
authors: Labas
authors: Christophe
authors: C
authors: Plomion
authors: Franck
authors: F
authors: Brignolas
authors: Domenico
authors: D
authors: Morabito
mesh_terms: D055864:Droughts; D015180:Electrophoresis, Gel, Two-Dimensional; D014644:Genetic Variation; D018384:Oxidative Stress; D018515:Plant Leaves / Q000235:genetics / Q000502:physiology; D032107:Populus / Q000201:enzymology / Q000235:genetics* / Q000378:metabolism*; D040901:Proteomics; D014867:Water / Q000378:metabolism*
chemical_list: D014867:Water