The inhibitory influence of adiponectin on the growth of the murine endothelial cell line HECa 10 in vitro.
BACKGROUND
Adiponectin, a peptide hormone secreted from the adipose tissue, has anti-diabetic, anti-atherogenic, and anti-inflammatory properties and is also involved in the regulation of angiogenesis. However, there are discrepancies among the results of the published data regarding its pro- or anti-angiogenic properties. The aim of our study was to examine the direct effect of various adiponectin concentrations applied separately or in combination with thalidomide on the growth of the murine endothelial cell line HECa 10 in 24- and 72-hour cell cultures.


METHODS
We used immortalized murine endothelial cell line received from endothelial cells of the mouse peripheral lymph node. The effect of adiponectin was examined at concentrations from 10(-5) to 10(-12)M. Thalidomide was used at 10(-3)M concentration. The growth of HECa10 cells was assessed by the colorimetric Mosmann method.


RESULTS
We found that adiponectin inhibited the growth of HECa 10 line at all examined concentrations in the 24-hour culture, with moderate potency. There were no dose- or time-response effects. In the 72-hour cell culture, adiponectin inhibited the growth with the same or weaker potency and we did not observe its inhibitory effect at 10(-12)M concentration. There was no beneficial interaction between adiponectin and thalidomide. In this study, however, thalidomide alone did not cause any inhibitory effect on this cell line.


CONCLUSIONS
The obtained data show that adiponectin inhibits endothelial cell growth and may participate in angiogenesis regulation as an endogenous antiangiogenic factor.
Połowinczak-Przybyłek
Joanna
J
Department of the Proliferative Diseases, Regional Oncological Centre, Łódź. g.m-mucha@wp.pl
Mełeń-Mucha
Gabriela
G
D052242:Adiponectin / Q000378:metabolism* / Q000494:pharmacology*; D000818:Animals; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D042783:Endothelial Cells / Q000166:cytology*; D051379:Mice; D018919:Neovascularization, Physiologic / Q000502:physiology*; D013792:Thalidomide / Q000008:administration & dosage
D052242:Adiponectin; D013792:Thalidomide