Controlled gene expression in the plant pathogen Cryphonectria parasitica by use of a copper-responsive element.
We have developed a tool for controlled expression of heterologous or ectopic genes in the chestnut pathogen Cryphonectria parasitica using the promoter region from a putative copper-regulated transporter gene. In addition, we have found that expression control via this system is not affected by the virulence-attenuating hypovirus CHV1-EP713.
Willyerd
Karyn L
KL
Department of Biology, New Mexico State University, Las Cruces, NM 88003, USA.
Kemp
Amanda M
AM
Dawe
Angus L
AL
D001203:Ascomycota / Q000235:genetics / Q000378:metabolism / Q000472:pathogenicity / Q000821:virology; D003300:Copper / Q000494:pharmacology*; D005656:Fungal Proteins / Q000235:genetics* / Q000378:metabolism; D015966:Gene Expression Regulation, Fungal / Q000187:drug effects*; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D054884:Host-Pathogen Interactions; D008969:Molecular Sequence Data; D010935:Plant Diseases / Q000821:virology*; D011401:Promoter Regions, Genetic; D012328:RNA Viruses / Q000502:physiology; D017422:Sequence Analysis, DNA; D014774:Virulence
D005656:Fungal Proteins; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins; D003300:Copper