title: Determination of nerve agent degradation products by capillary electrophoresis using field-amplified sample stacking injection with the electroosmotic flow pump and contactless conductivity detection.
abstract: In the present study, field-amplified sample stacking injection using the electroosmotic flow pump (FAEP) was developed for the capillary electrophoretic separation of the four nerve agent degradation products methylphosphonic acid (MPA), ethyl methylphosphonic acid (EMPA), isopropyl methylphosphonic acid (IMPA) and cyclohexyl methylphosphonic acid (CMPA). Coupled to contactless conductivity detection, direct quantification of these non-UV active compounds could be achieved. Sensitivity enhancement of up to 500 to 750-fold could be obtained. The newly established approach was applied to the determination of the analytes in river water and aqueous extracts of soil. Detection limits of 0.5, 0.7, 1.4 and 2.7 ng/mL were obtained for MPA, EMPA, IMPA and CMPA, respectively, in river water and 0.09, 0.14, 0.44 and 0.22 microg/g, respectively, in soil.
authors: College of Pharmacy, Tongji Medical Center, Huazhong University of Science and Technology, Wuhan 430030, China.
authors: Li
authors: L
authors: Xu
authors: Peter C
authors: PC
authors: Hauser
authors: Hian Kee
authors: HK
authors: Lee
mesh_terms: D000077286:Cetrimonium; D002593:Cetrimonium Compounds / Q000737:chemistry; D002619:Chemical Warfare Agents / Q000032:analysis*; D004553:Electric Conductivity; D053841:Electroosmosis / Q000295:instrumentation / Q000379:methods*; D019075:Electrophoresis, Capillary / Q000295:instrumentation / Q000379:methods*; D016014:Linear Models; D000432:Methanol / Q000737:chemistry; D063065:Organophosphonates / Q000032:analysis; D009943:Organophosphorus Compounds / Q000032:analysis*; D015203:Reproducibility of Results; D045483:Rivers / Q000737:chemistry; D012680:Sensitivity and Specificity; D012989:Soil Pollutants / Q000032:analysis; D014867:Water / Q000737:chemistry; D014874:Water Pollutants, Chemical / Q000032:analysis
chemical_list: D002593:Cetrimonium Compounds; D002619:Chemical Warfare Agents; D063065:Organophosphonates; D009943:Organophosphorus Compounds; D012989:Soil Pollutants; D014874:Water Pollutants, Chemical; D014867:Water; C108318:ethyl methylphosphonic acid; C048760:isopropyl methylphosphonic acid; C073636:cyclohexyl methylphosphonic acid; C032627:methylphosphonic acid; D000432:Methanol; D000077286:Cetrimonium