[Toxicity evaluation of sewage treatment plant effluent of chemical industrial park along the Yangtze River on rat testicular germ cells in vitro].
By using rat testicular germ cells in vitro toxicity testing method based on original cells culture, the reproduction toxicity of sewage treatment plant effluent of Chemical Industrial Park along the Yangtze River was evaluated, through cells changes in morphologic, activity and viability parameters. The results showed that both of the effluents from new developed Chemical Industrial Park A and provincial Chemical Industrial Park B contain reproductive toxic substances. The toxicity of Park A has more significant undergone changes in cells activity of sertoli cells (p < 0.01), spermatogenic cells (p < 0.05) and leyding cells (p < 0.05), lactate dehydrogenase activity (p < 0.01) and testosterone secretion (p < 0.01) than that of Park B. Sepermatogenic cells are more sensitive in indicating reproduction toxicity for testicular, compared with leyding cells and sertoli cells. This study demonstrated that, as an indispensable and complementary tool for water quality assessment, rat testicular germ cells in vitro toxicity testing based on original cells culture can be used to comprehensively evaluate the reproduction toxicity of sewage treatment plant effluent, and provide prompt and useful discharge quality information.
Hu
Guan-Jiu
GJ
State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing 210093, China. fanfan_67946@163.com
Wang
Xiao-Yi
XY
Shi
Wei
W
Bai
Chou-Yong
CY
Wu
Jiang
J
Liu
Hong-Ling
HL
Yu
Hong-Xia
HX
D000818:Animals; D002478:Cells, Cultured; D005854:Germ Cells / Q000166:cytology / Q000187:drug effects*; D007220:Industrial Waste; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D012722:Sewage / Q000009:adverse effects; D013737:Testis / Q000166:cytology*; D013739:Testosterone / Q000378:metabolism; D018675:Toxicity Tests; D014865:Waste Disposal, Fluid / Q000379:methods*; D014874:Water Pollutants, Chemical / Q000633:toxicity*
D007220:Industrial Waste; D012722:Sewage; D014874:Water Pollutants, Chemical; D013739:Testosterone