title: Organoselenides from Nicotiana tabacum genetically modified to accumulate selenium.
abstract: Nicotiana tabacum L. (tobacco) plants were transformed to overexpress a selenocysteine methyltransferase gene from the selenium hyperaccumulator Astragalus bisulcatus (Hook.) A. Gray (two-grooved milkvetch), and an ATP-sulfurylase gene from Brassica oleracea L. var. italica (broccoli). Solvent extraction of leaves harvested from plants treated with selenate revealed five selenium-containing compounds, of which four were identified by chemical synthesis as 2-(methylseleno)acetaldehyde, 2,2-bis(methylseleno)acetaldehyde, 4-(methylseleno)-(2E)-nonenal, and 4-(methylseleno)-(2E,6Z)-nonadienal. These four compounds have not previously been reported in nature.
authors: The New Zealand Institute for Plant and Food Research Limited (Plant and Food Research), Private Bag 11600, Palmerston North 4442, New Zealand. Electronic address: amatich@hortresearch.co.nz.
authors: Adam J
authors: AJ
authors: Matich
authors: The New Zealand Institute for Plant and Food Research Limited (Plant and Food Research), Private Bag 11600, Palmerston North 4442, New Zealand.
authors: Marian J
authors: MJ
authors: McKenzie
authors: The New Zealand Institute for Plant and Food Research Limited (Plant and Food Research), Private Bag 11600, Palmerston North 4442, New Zealand.
authors: David A
authors: DA
authors: Brummell
authors: The New Zealand Institute for Plant and Food Research Limited (Plant and Food Research), Private Bag 11600, Palmerston North 4442, New Zealand.
authors: Daryl D
authors: DD
authors: Rowan
mesh_terms: D027883:Astragalus Plant / Q000201:enzymology / Q000235:genetics; D008780:Methyltransferases / Q000378:metabolism*; D015394:Molecular Structure; D016566:Organoselenium Compounds / Q000737:chemistry / Q000302:isolation & purification*; D018515:Plant Leaves / Q000737:chemistry; D030821:Plants, Genetically Modified / Q000737:chemistry* / Q000235:genetics*; D012643:Selenium / Q000737:chemistry / Q000378:metabolism*; D014026:Nicotiana / Q000737:chemistry* / Q000235:genetics*
chemical_list: D016566:Organoselenium Compounds; D008780:Methyltransferases; C100751:selenocysteine methyltransferase; D012643:Selenium