Electron-transfer kinetics of microperoxidase-11 covalently immobilised onto the surface of multi-walled carbon nanotubes by reactive landing of mass-selected ions.
Controlled deposition of biological molecules on nanostructured materials is a basic step towards the realisation of biochip components. In this study we report the investigation of the first covalent immobilisation of mass-selected redox protein on a carboxyl-functionalised multi-walled carbon nanotube (MWCNT) electrode surface by means of ion soft landing. The immobilised protein maintains its biochemical properties, displaying an excellent electrochemical behaviour on the electrode surface. The deposition of mass-selected ions is influenced by several factors, including the charge state and the collision energy of the projectile ions. To elucidate the mechanism involved in the protein reactive landing onto the MWCNT surface, the data obtained from cyclic voltammetry experiments were modelled according to the Marcus theory. The proposed method opens up the way to the development of a new generation of biocomponents with potential use in biosensors, diagnostics, biofuel cells and bioactive films.
Mazzei
Franco
F
Sapienza University of Rome, Department of Chemistry and Drug Technologies, P.le Aldo Moro 5, 00185 Rome, Italy. Franco.Mazzei@uniroma1.it
Favero
Gabriele
G
Frasconi
Marco
M
Tata
Alessandra
A
Pepi
Federico
F
D015374:Biosensing Techniques; D004566:Electrodes; D004579:Electron Transport; D004800:Enzymes, Immobilized / Q000737:chemistry*; D006418:Heme / Q000737:chemistry; D007477:Ions / Q000737:chemistry; D007501:Iron / Q000737:chemistry; D008956:Models, Chemical; D037742:Nanotubes, Carbon / Q000737:chemistry*; D010544:Peroxidases / Q000737:chemistry* / Q000378:metabolism
D004800:Enzymes, Immobilized; D007477:Ions; D037742:Nanotubes, Carbon; D006418:Heme; D007501:Iron; D010544:Peroxidases; C021799:microperoxidase