Study of anaerobic lactate metabolism under biosulfidogenic conditions.
Biological sulfate reduction (BSR) has been reported to have potential for the treatment of acid mine drainage (AMD). The provision of a suitable carbon source and electron donor for this process remains a challenge. Lactate offers potential advantages as carbon source and electron donor in the biological sulfate reduction process. As this substrate is utilized by both fermentative bacteria and oxidative sulfate-reducing bacteria (SRB), the effect of feed sulfate concentration on the lactate pathways utilized under biosulfidogenic conditions was investigated. Studies were carried out in chemostat bioreactors across a range of residence times, using an enriched culture of SRB. The stoichiometry of biological sulfate reduction was affected by feed sulfate concentration and dilution rate. Incomplete oxidation of lactate was dominant at low feed sulfate concentration (1.0 g/L), while the yield of propionate from lactate metabolism increased at feed sulfate concentrations of 2.5-10.0 g/L, indicating the occurrence of lactate fermentation. Furthermore, at each sulfate feed concentration, in the range 2.5-10.0 g/L, the ratio in which lactate was metabolized by the oxidative and fermentative pathways varied with varying dilution rates. Lactate oxidation was higher at a feed sulfate concentration of 10.0 g/L relative to 2.5 and 5.0 g/L. The volumetric lactate utilization rate was enhanced by increasing the feed sulfate concentration. However, the proportion of total lactate consumed that was channelled into providing electrons for other activities apart from sulfate reduction also increased over the range of increasing sulfate concentrations studied and appeared to be a function of residual lactate and sulfide concentrations.
Oyekola
Oluwaseun O
OO
Centre for Bioprocess Engineering Research, Department of Chemical Engineering, University of Cape Town, Private Bag X3, Rondebosch 7701, South Africa.
van Hille
Robert P
RP
Harrison
Susan T L
ST
D000693:Anaerobiosis; D019149:Bioreactors / Q000382:microbiology*; D005285:Fermentation; D007700:Kinetics; D019344:Lactic Acid / Q000378:metabolism*; D010084:Oxidation-Reduction; D013431:Sulfates / Q000378:metabolism*; D013440:Sulfides / Q000378:metabolism*; D013816:Thermodynamics
D013431:Sulfates; D013440:Sulfides; D019344:Lactic Acid