title: The effect of beta interferon on dendritic cells and cytokine synthesis by CD4+ T cells.
abstract: BACKGROUND
  Dendritic cells (DC) are a key regulator of the immune response, and interferon-beta (IFN-beta) is considered an immunomodulatory molecule for DC.
  
  
  OBJECTIVE
  The purpose of this study was to evaluate the ability of IFN-beta treated DC to induce cytokine secretion by CD4+ T cells.
  
  
  METHODS
  Dendritic cells were generated from blood monocytes with granulocyte-monocyte colony-stimulating factor and interleukin-4 with or without IFN-beta. We analyzed the production of CD4+ T helper cytokines (IL-17, IFN-gamma and IL-10) in the supernatant of the dendritic cell-T cell co- cultures by ELISA. We also studied the effects of HLA-G and costimulatory molecules on immature and mature DC.
  
  
  RESULTS
  IFN-gamma and IL-17 decreased significantly in the presence of HLA-G-bearing DC compared to control cultures (p<0.05).
  
  
  CONCLUSIONS
  Using the mixed leukocyte reaction, we found that DC treated with IFN-beta mediated the inhibition of T cell activation via cytokine production. We conclude that this is important for preventing overactivation of the immune system.
authors: Department of Microbiology and Immunology, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran. abedianlab@yahoo.co.uk
authors: Saeid
authors: S
authors: Abediankenari
authors: Davoud
authors: D
authors: Shaker
authors: Farshideh
authors: F
authors: Abedian
authors: Arazmohammad
authors: A
authors: Mirabi
mesh_terms: D015496:CD4-Positive T-Lymphocytes / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D002450:Cell Communication / Q000276:immunology; D002454:Cell Differentiation; D018920:Coculture Techniques; D003713:Dendritic Cells / Q000187:drug effects / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D004797:Enzyme-Linked Immunosorbent Assay; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000276:immunology / Q000378:metabolism; D006680:HLA Antigens / Q000378:metabolism; D059951:HLA-G Antigens; D015395:Histocompatibility Antigens Class I / Q000378:metabolism; D006801:Humans; D016899:Interferon-beta / Q000276:immunology / Q000378:metabolism* / Q000494:pharmacology; D007371:Interferon-gamma / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D007378:Interleukins / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D008213:Lymphocyte Activation / Q000187:drug effects; D007959:Lymphocyte Culture Test, Mixed; D009000:Monocytes / Q000473:pathology
chemical_list: D006680:HLA Antigens; D059951:HLA-G Antigens; D015395:Histocompatibility Antigens Class I; D007378:Interleukins; D016899:Interferon-beta; D007371:Interferon-gamma; D016178:Granulocyte-Macrophage Colony-Stimulating Factor