Assessment of liver function in dogs using the 13C-galactose breath test.
The aim of this study was to evaluate the application of the 13C-galactose breath test (13C-GBT) in assessing canine liver function by applying it to a group of healthy dogs, and to a group with clinicopathological evidence of liver dysfunction. Breath samples were collected 30 min before ingestion of 13C-galactose, and then at regular intervals thereafter for 6 h. The proportion of 13CO2/12CO2 in the breath samples was measured by isotope-ratio mass spectrometry. There was no significant difference in recovery of 13CO2 in the diseased group, compared to the healthy controls, but there was considerable inter-subject variation in both groups, possibly due to differences in the rate of gastric emptying, which could preclude detection of alterations in hepatic metabolism of galactose. The results of this study do not support the application of the 13C-GBT for assessment of canine liver function.
Silva
S
S
Division of Companion Animal Studies, Department of Clinical Veterinary Science, University of Bristol, Langford House, Langford, Bristol, BS40 5DU, UK. Susana.Silva@bristol.ac.uk
Wyse
C A
CA
Goodfellow
M R
MR
Yam
P S
PS
Preston
T
T
Papasouliotis
K
K
Hall
E J
EJ
D000818:Animals; D001944:Breath Tests; D002245:Carbon Dioxide / Q000032:analysis; D002247:Carbon Isotopes; D016022:Case-Control Studies; D004283:Dog Diseases / Q000175:diagnosis* / Q000378:metabolism; D004285:Dogs; D005260:Female; D005690:Galactose / Q000378:metabolism*; D008107:Liver Diseases / Q000175:diagnosis / Q000378:metabolism / Q000662:veterinary*; D008111:Liver Function Tests / Q000662:veterinary*; D008297:Male
D002247:Carbon Isotopes; D002245:Carbon Dioxide; D005690:Galactose