[Protective effect of polysaccharides from sea cucumber on glu-induced neurotoxicity in PC12 cells].
OBJECTIVE
To investigate the protective effect of polysaccharides from sea cucumber (HS) on Glu-induced neurotoxicity in PC12 cells.


METHODS
The toxicity model was established by treating PC12 cells with Glu in vitro. Cell viability was assayed with MTT method. The vitality of antioxidant enzymes (SOD) and the level of malondialdehyde (MDA) and lactic dehydrogenase (LDH) were evaluated by chromatometry.


RESULTS
The vitality of SOD increased and the levels of MDA and LDH significantly decreased in PC12 cells of HS group compared with the model group. HS in different concentration showed protective effects as it increased the vitality of SOD and decreased the content of MDA and LDH in a dose-dependent manner.


CONCLUSIONS
Polysaccharide from sea cucumber can protect PC12 cells from the injury induced by Glu.
Diao
Hong-Xia
HX
Weihai International Biotechnology R & D Center of Shandong University, Weihai 264209, China.
Song
Shu-Liang
SL
Liang
Hao
H
Wang
Yun-Shan
YS
Wang
Wei-Li
WL
Ji
Ai-Guo
AG
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D002470:Cell Survival / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D005971:Glutamates / Q000633:toxicity; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008315:Malondialdehyde / Q000378:metabolism; D018696:Neuroprotective Agents / Q000494:pharmacology*; D018384:Oxidative Stress / Q000187:drug effects; D016716:PC12 Cells; D011134:Polysaccharides / Q000494:pharmacology*; D051381:Rats; D012616:Sea Cucumbers / Q000737:chemistry*; D013482:Superoxide Dismutase / Q000378:metabolism
D000975:Antioxidants; D005971:Glutamates; D018696:Neuroprotective Agents; D011134:Polysaccharides; D008315:Malondialdehyde; D007770:L-Lactate Dehydrogenase; D013482:Superoxide Dismutase