Conservation across species identifies several transcriptional enhancers in the HEX genomic region.
The HEX gene encodes for a homeodomain-containing transcription factor that controls various phases of vertebrate development. During development, as well as in adult, HEX is expressed in several different tissues including thyroid, liver, lung, mammary gland, haematopoietic progenitors, and endothelial cells, suggesting that this gene is subjected to a complex transcriptional regulation. In this study, we have evaluated the presence of different enhancers in the HEX gene region by using a phylogenetic approach. Several non-coding sequences, conserved between human and mouse, were selected. Four conserved sequences showed enhancer activity in MCF-7 cells. Two of these enhancers (located in the first and third intron, respectively) have been previously identified by other experimental approaches. These elements, as well as one among the new identified enhancers (located 2 kb 3' to the HEX gene), are able to activate the HEX minimal promoter "in trans." The activity of the 3' enhancer was strongly reduced by overexpression of HDAC3.
D'Elia
Angela Valentina
AV
Azienda Ospedaliero-Universitaria S. Maria della Misericordia Udine, Udine, Italy.
Bregant
Elisa
E
Passon
Nadia
N
Puppin
Cinzia
C
Meneghel
Alessia
A
Damante
Giuseppe
G
D000328:Adult; D000818:Animals; D002478:Cells, Cultured; D047369:Chromatin Immunoprecipitation; D017124:Conserved Sequence; D017931:DNA Primers / Q000737:chemistry; D004742:Enhancer Elements, Genetic / Q000235:genetics*; D005786:Gene Expression Regulation; D016678:Genome; D006655:Histone Deacetylases / Q000378:metabolism; D018398:Homeodomain Proteins / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D051379:Mice; D010802:Phylogeny; D012045:Regulatory Sequences, Nucleic Acid; D014158:Transcription, Genetic / Q000235:genetics*; D000099186:Histone Deacetylase 3
D017931:DNA Primers; D006655:Histone Deacetylases; D018398:Homeodomain Proteins; D000099186:Histone Deacetylase 3