Evaluation of MeningoFinder, a novel multiplex ligation-dependent probe amplification assay for simultaneous detection of six virus species causing central nervous system infections.
A multiplex ligation-dependent probe amplification assay for simultaneous detection of six virus species was developed and tested on clinical cerebrospinal fluid (CSF) samples. The assay, termed MeningoFinder, showed an accordance of 97%, concordance of 96%, interlaboratory sensitivity of 90%, and interlaboratory specificity of 94% compared to PCRs.
Wolffs
P F G
PF
Department of Medical Microbiology, Maastricht University Medical Center, The Netherlands. p.wolffs@mumc.nl
Vink
C
C
Keijdener
J
J
Habek
B
B
Reijans
M
M
Simons
G
G
Bruggeman
C A
CA
van den Brule
A J C
AJ
D001431:Bacteriological Techniques / Q000379:methods*; D002494:Central Nervous System Infections / Q000821:virology*; D002555:Cerebrospinal Fluid / Q000821:virology; D006801:Humans; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D014777:Virus Diseases / Q000175:diagnosis*; D014780:Viruses / Q000235:genetics / Q000302:isolation & purification*