Fluorescent cassettes for monitoring three-component interactions in vitro and in living cells.
Two water-soluble "through-bond energy transfer cassettes" (TBET-cassettes) were prepared. They have good extinction coefficients at the donor part and transfer energy to the acceptor parts with good "overall quantum yields" (0.30 and 0.24 in pH 7.4 phosphate buffer). Fluorescence resonance energy transfer from one protein functionalized with an appropriate donor can be used to excite the TBET-cassettes on different proteins to probe protein-protein interactions under conditions that would not be possible for single-dye acceptor systems.
Wu
Liangxing
L
Department of Chemistry, Texas A & M University, Box 30012, College Station, Texas 77841, USA.
Loudet
Aurore
A
Barhoumi
Rola
R
Burghardt
Robert C
RC
Burgess
Kevin
K
D000818:Animals; D001360:Avidin / Q000032:analysis / Q000737:chemistry / Q000378:metabolism*; D019921:Biotinylation; D019556:COS Cells; D002417:Cattle; D002522:Chlorocebus aethiops; D031541:Fluorescence Resonance Energy Transfer; D005456:Fluorescent Dyes / Q000737:chemistry*; D025941:Protein Interaction Mapping / Q000379:methods*; D012710:Serum Albumin, Bovine / Q000032:analysis / Q000737:chemistry / Q000378:metabolism*; D019809:Streptavidin / Q000032:analysis / Q000737:chemistry / Q000378:metabolism*
D005456:Fluorescent Dyes; D001360:Avidin; D012710:Serum Albumin, Bovine; D019809:Streptavidin