title: Genotyping and in vitro antifungal susceptibility of Neoscytalidium dimidiatum isolates from different origins.
abstract: This study evaluated the genetic variability and in vitro susceptibility patterns of isolates of Neoscytalidium dimidiatum and Scytalidium hyalinum from different geographical origins. Partial sequences of four loci (the ITS region and D1/D2 domains of the 28S rRNA gene and the tubulin and chitin synthase genes) were analysed. Among a total of 1970 bp sequenced in 24 isolates, 7 polymorphic positions (0.36%) were detected, representing five different sequence types (ST1-ST5), from which two (ST2 and ST3) were detected exclusively in isolates from plants, two (ST1 and ST5) were found only in clinical isolates and one (ST4) was observed in isolates from humans and from a mango tree. We propose subordinating S. hyalinum as a variety of N. dimidiatum. Amphotericin B was the most active drug, but low minimum inhibitory concentrations were also detected for voriconazole, terbinafine and anidulafungin.
authors: Unitat de Microbiologia, Facultat de Medicina i Ciències de la Salut, Universitat Rovira i Virgili, Reus, Spain.
authors: Hugo
authors: H
authors: Madrid
authors: Mery
authors: M
authors: Ruíz-Cendoya
authors: Josep
authors: J
authors: Cano
authors: Alberto
authors: A
authors: Stchigel
authors: Rosane
authors: R
authors: Orofino
authors: Josep
authors: J
authors: Guarro
mesh_terms: D000935:Antifungal Agents / Q000494:pharmacology*; D001203:Ascomycota / Q000145:classification / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification; D004271:DNA, Fungal / Q000032:analysis; D004275:DNA, Ribosomal / Q000032:analysis; D005656:Fungal Proteins / Q000235:genetics; D005838:Genotype; D006801:Humans; D031022:Mangifera / Q000382:microbiology*; D008826:Microbial Sensitivity Tests; D003904:Mitosporic Fungi / Q000145:classification / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification; D016533:Mycological Typing Techniques; D009181:Mycoses / Q000382:microbiology*; D010944:Plants / Q000382:microbiology*; D016133:Polymerase Chain Reaction / Q000379:methods; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D012339:RNA, Ribosomal, 28S / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D000935:Antifungal Agents; D004271:DNA, Fungal; D004275:DNA, Ribosomal; D005656:Fungal Proteins; D012339:RNA, Ribosomal, 28S