[Increased efficiency of recombinant proteins production in plants due to optimized translation of RNA of viral vector].
One of the most efficient methods for fast and efficient production of the target proteins in plants is based on the use of self-replicating recombinant viral vectors. We constructed phytoviral vector based on the genome of potato X virus containing the sequence of 5'-untranslated region of RNA 4 of alfalfa mosaic virus immediately upstream of the target gene. We demonstrated that incorporation of this sequence into the viral vector results in 3-4 fold elevation of the level of production of the target protein in plant due to increased efficiency of translation of viral subgenomic RNA comprising the target gene. The new vector may be used for production of recombinant proteins in plants.
Mardanova
E S
ES
Kotliarov
R Iu
RIu
Ravin
N V
NV
D017794:Alfalfa mosaic virus / Q000235:genetics; D005822:Genetic Vectors; D030821:Plants, Genetically Modified / Q000235:genetics / Q000378:metabolism*; D017863:Potexvirus / Q000235:genetics*; D012367:RNA, Viral / Q000096:biosynthesis*; D011994:Recombinant Proteins / Q000096:biosynthesis*; D012231:Rhizobium / Q000235:genetics; D014026:Nicotiana / Q000235:genetics / Q000378:metabolism*; D014162:Transfection
D012367:RNA, Viral; D011994:Recombinant Proteins