title: X-ray crystallographic studies of RNase A variants engineered at the most destabilizing positions of the main hydrophobic core: further insight into protein stability.
abstract: To investigate the structural origin of decreased pressure and temperature stability, the crystal structure of bovine pancreatic ribonuclease A variants V47A, V54A, V57A, I81A, I106A, and V108A was solved at 1.4-2.0 A resolution and compared with the structure of wild-type protein. The introduced mutations had only minor influence on the global structure of ribonuclease A. The structural changes had individual character that depends on the localization of mutated residue, however, they seemed to expand from mutation site to the rest of the structure. Several different parameters have been evaluated to find correlation with decrease of free energy of unfolding DeltaDeltaG(T), and the most significant correlation was found for main cavity volume change. Analysis of the difference distance matrices revealed that the ribonuclease A molecule is organized into five relatively rigid subdomains with individual response to mutation. This behavior consistent with results of unfolding experiments is an intrinsic feature of ribonuclease A that might be surviving remnants of folding intermediates and reflects the dynamic nature of the molecule.
authors: Faculty of Chemistry, Jagiellonian University, Ingardena 3, Kraków 30-060, Poland.
authors: Katarzyna
authors: K
authors: Kurpiewska
authors: Josep
authors: J
authors: Font
authors: Marc
authors: M
authors: Ribó
authors: Maria
authors: M
authors: Vilanova
authors: Krzysztof
authors: K
authors: Lewiński
mesh_terms: D000818:Animals; D002417:Cattle; D018360:Crystallography, X-Ray / Q000379:methods; D004926:Escherichia coli / Q000378:metabolism; D008958:Models, Molecular; D016296:Mutagenesis; D016297:Mutagenesis, Site-Directed; D009154:Mutation; D010179:Pancreas / Q000201:enzymology; D017510:Protein Folding; D017433:Protein Structure, Secondary; D012259:Ribonuclease, Pancreatic / Q000737:chemistry*; D012260:Ribonucleases / Q000737:chemistry; D013696:Temperature; D013816:Thermodynamics
chemical_list: D012260:Ribonucleases; D012259:Ribonuclease, Pancreatic