title: Label free capacitive immunosensor for detecting calpastatin--a meat tenderness biomarker.
abstract: An immunological capacitive biosensor for calpastatin was developed, optimized and applied for the analysis of meat extract samples. Anti-calpastatin antibody was immobilized on a gold electrode modified with a self-assembled monolayer of mercaptoundecanoic acid and Protein A from Staphylococcus aureus, and the obtained immunosensor was inserted as the working electrode in an electrochemical cell of a flow injection system. The dynamic range of the sensor was 20 to 160 ng/mL calpastatin. The electrode could be regenerated and re-used for more than 7 days with minimal reduction in sensitivity. For the analysis of real samples, the target analyte was extracted from the Longissimus dorsi muscle from beef carcasses directly after slaughtering. The extract was analyzed both with the developed immunosensor and microtiter plate ELISA, and a good correlation was obtained. However the immunosensor offers advantages of speed, simplicity, sensitivity and possibility for miniaturization over conventional assays for calpastatin quantification.
authors: Department of Analytical Chemistry, University of Lund, 221 00 Lund, Sweden. Kinga.Zor@analykem.lu.se
authors: Kinga
authors: K
authors: Zór
authors: Roberto
authors: R
authors: Ortiz
authors: Ebru
authors: E
authors: Saatci
authors: Ronald
authors: R
authors: Bardsley
authors: Tim
authors: T
authors: Parr
authors: Elisabeth
authors: E
authors: Csöregi
authors: Mihaela
authors: M
authors: Nistor
mesh_terms: D000818:Animals; D055910:Antibodies, Immobilized / Q000276:immunology; D015415:Biomarkers / Q000032:analysis; D015374:Biosensing Techniques / Q000379:methods*; D002135:Calcium-Binding Proteins / Q000032:analysis* / Q000276:immunology; D002417:Cattle; D025802:Electric Capacitance; D004566:Electrodes; D019649:Food Industry; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D008460:Meat; D051379:Mice; D013194:Staining and Labeling; D013499:Surface Properties; D013997:Time Factors
chemical_list: D055910:Antibodies, Immobilized; D015415:Biomarkers; D002135:Calcium-Binding Proteins; C033668:calpastatin