Evaluation of genome-derived amplicon length polymorphism PCR primers for the genetic evaluation of related strains of Salmonella.
OBJECTIVE
The intent of this study is to exploit both the genetic diversity and conservation demonstrated between the Salmonella enterica ssp. enterica serovar Typhi CT18 and Salm. enterica ssp. enterica serovar Typhimurium LT2 genomes by utilizing amplicon length polymorphism (ALP) to detect and differentiate various Salmonella strains.


RESULTS
Methods of ALP-PCR analysis were developed based on identifying DNA sequence deletions within highly homologous regions of the Salm. Typhi CT18 and Salm. Typhimurium LT2 genomes. This study describes the application of genome-based ALP-PCR using primer pairs designed to detect genomic differences present within both Salmonella genomes and evaluated against a reference set of Salmonella strains.


CONCLUSIONS
This study defines a collection of primer sequences broadly distributed along the Salmonella genome that can differentiate various Salmonella strains.


CONCLUSIONS
Genome-based ALP-PCR provides a useful and powerful analytical method to evaluate variability within a group of Salmonella strains independent of serological, phenotypic or other molecular approaches.
Rice
W C
WC
USDA-ARS Conservation and Production Research Laboratory, Bushland, TX 79012-0010, USA. William.Rice@ars.usda.gov
D016000:Cluster Analysis; D017931:DNA Primers; D016680:Genome, Bacterial; D016133:Polymerase Chain Reaction / Q000379:methods*; D012475:Salmonella / Q000145:classification / Q000235:genetics*
D017931:DNA Primers