title: In vitro study on DNA binding of ruthenium(II) complexes with polypyridyl ligands.
abstract: A new ligand {2-(5-nitro-furan-2-yl)-1H-1,3,7,8-tetraaza-cyclopenta[l]-phenanthrene} (nftp) and its Ru(II) complexes [Ru(phen)(2)(nftp)](2+) (1) (phen = 1,10-phenanthroline) and [Ru(bpy)(2)(nftp)](2+) (2) (bpy = 2,2'-bipyridine) were synthesized and characterized. The binding properties of the two complexes to calf thymus-DNA (CT-DNA) were investigated by different spectrophotometric methods and viscosity measurements together with equilibrium dialysis and circular dichroism spectroscopy. The results suggest that both complexes bind to DNA through intercalation and enantioselectively interact with CT-DNA. However, complex 1 is a better candidate as an enantioselective binder to CT-DNA than complex 2. Although no emission is generally observed in water or organic solvents for Ru(II) polypyridyl complexes with a nitro group, complexes 1 and 2 can emit luminescence in both media. When irradiated at 365 nm, complex 1 cleaves DNA more effectively than complex 2.
authors: College of Chemistry, Xiangtan University, Xiangtan, People's Republic of China. lfwyxh@yahoo.com.cn
authors: Li-Feng
authors: LF
authors: Tan
authors: JianLiang
authors: J
authors: Shen
authors: XueJiao
authors: X
authors: Chen
authors: XiLing
authors: X
authors: Liang
mesh_terms: D015082:2,2'-Dipyridyl / Q000737:chemistry*; D000818:Animals; D001665:Binding Sites; D002417:Cattle; D002942:Circular Dichroism; D004247:DNA / Q000737:chemistry*; D007364:Intercalating Agents / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy; D008956:Models, Chemical; D015394:Molecular Structure; D009942:Organometallic Compounds / Q000138:chemical synthesis / Q000737:chemistry*; D010616:Phenanthrenes / Q000737:chemistry; D010618:Phenanthrolines / Q000737:chemistry; D012428:Ruthenium / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D013237:Stereoisomerism
chemical_list: D007364:Intercalating Agents; D009942:Organometallic Compounds; D010616:Phenanthrenes; D010618:Phenanthrolines; C031181:phenanthrene; D015082:2,2'-Dipyridyl; D012428:Ruthenium; D004247:DNA; C427203:calf thymus DNA; C025205:1,10-phenanthroline