High-throughput sequence analysis of variants of human cytomegalovirus strains Towne and AD169.
The genomes of commonly used variants of human cytomegalovirus (HCMV) strains Towne and AD169 each contain a substantial mutation in which a region (U(L)/b') at the right end of the long unique region has been replaced by an inverted duplication of a region from the left end of the genome. Using high-throughput technology, we have sequenced HCMV strain Towne (ATCC VR-977) and confirmed the presence of two variants, one exhibiting the replacement in U(L)/b' and the other intact in this region. Both variants are mutated in genes RL13, UL1, UL40, UL130, US1 and US9. We have also sequenced a novel AD169 variant (varUC) that is intact in U(L)/b' except for a small deletion that affects genes UL144, UL142, UL141 and UL140. Like other AD169 variants, varUC is mutated in genes RL5A, RL13, UL36 and UL131A. A subpopulation of varUC contains an additional deletion affecting genes IRS1, US1 and US2.
Bradley
Amanda J
AJ
MRC Virology Unit, Institute of Virology, Church Street, Glasgow G11 5JR, UK.
Lurain
Nell S
NS
Department of Immunology and Microbiology, Rush University Medical Center, 1653 West Congress Parkway, Chicago, IL 60612, USA.
Ghazal
Peter
P
Division of Pathway Medicine, University of Edinburgh Medical School, Chancellor's Building, 49 Little France Crescent, Edinburgh EH16 4SB, UK.
Trivedi
Urmi
U
The Gene Pool, Ashworth Laboratories, Institute of Evolutionary Biology, King's Buildings, Edinburgh EH9 3JT, UK.
Cunningham
Charles
C
MRC Virology Unit, Institute of Virology, Church Street, Glasgow G11 5JR, UK.
Baluchova
Katarina
K
MRC Virology Unit, Institute of Virology, Church Street, Glasgow G11 5JR, UK.
Gatherer
Derek
D
MRC Virology Unit, Institute of Virology, Church Street, Glasgow G11 5JR, UK.
Wilkinson
Gavin W G
GWG
Department of Medical Microbiology, Tenovus Building, School of Medicine, Cardiff University, Heath Park, Cardiff CF14 4XX, UK.
Dargan
Derrick J
DJ
MRC Virology Unit, Institute of Virology, Church Street, Glasgow G11 5JR, UK.
Davison
Andrew J
AJ
MRC Virology Unit, Institute of Virology, Church Street, Glasgow G11 5JR, UK.
D001483:Base Sequence; D003587:Cytomegalovirus / Q000145:classification* / Q000235:genetics*; D004279:DNA, Viral; D014644:Genetic Variation; D016679:Genome, Viral; D006801:Humans; D009154:Mutation
D004279:DNA, Viral