Characterization of an adenylate cyclase gene (cyaB) deletion mutant of Corynebacterium glutamicum ATCC 13032.
Genome analysis of C. glutamicum ATCC 13032 has showed one putative adenylate cyclase gene, cyaB (cg0375) which encodes membrane protein belonging to class III adenylate cyclases. To characterize the function of cyaB, a deletion mutant was constructed, and the mutant showed decreased level of intracellular cyclic AMP compared to that of wild-type. Interestingly, the cyaB mutant displayed growth defect on acetate medium, and this effect was reversed by complementation with cyaB gene. Similarly, it showed growth defect on glucose-acetate mixture minimal medium, and the utilization of glucose was retarded in the presence of acetate. The deletion mutant retained the activity of glyoxylate bypass enzymes. Additionally, the mutant could grow on ethanol but not on propionate medium. The data obtained from this study suggests that adenylate cyclase plays an essential role in the acetate metabolism of C. glutamicum, even though detailed regulatory mechanisms involving cAMP are not yet clearly defined. The observation that glyoxylate bypass enzymes are derepressed in cyaB mutant indicates the involvement of cAMP in the repression of aceB and aceA.
Cha
Pu Hyeon
PH
Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon, Korea.
Park
Sun-Yang
SY
Park
Sun-Yung
SY
Moon
Min-Woo
MW
Subhadra
Bindu
B
Oh
Tae-Kwang
TK
Kim
Eungbin
E
Kim
Jihyun F
JF
Lee
Jung-Kee
JK
D000085:Acetates / Q000378:metabolism; D000262:Adenylyl Cyclases / Q000235:genetics / Q000378:metabolism*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D003001:Cloning, Molecular; D048230:Corynebacterium glutamicum / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development; D000242:Cyclic AMP / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics; D000431:Ethanol / Q000378:metabolism; D017353:Gene Deletion; D005798:Genes, Bacterial; D005947:Glucose / Q000378:metabolism; D008969:Molecular Sequence Data; D011422:Propionates / Q000378:metabolism
D000085:Acetates; D001426:Bacterial Proteins; D011422:Propionates; D000431:Ethanol; D000242:Cyclic AMP; D000262:Adenylyl Cyclases; D005947:Glucose