Antioxidant phenylpropanoid glycosides from Buddleja davidii.
Phytochemical investigations on the n-BuOH-soluble fraction of the whole plant of Buddleja davidii led to the isolation of the phenylpropanoid glycosides 1-10. Their structures were determined by 1D and 2D NMR spectroscopic techniques. All the compounds showed potent antioxidative activity in three different tests, with IC(50) values in the range 4.15-9.47 microM in the hydroxyl radical ( OH) inhibitory activity test, 40.32-81.15 microM in the total ROS (reactive oxygen species) inhibitory activity test, and 2.26-7.79 microM in the peroxynitrite (ONOO(-)) scavenging activity test. Calceolarioside A (1) displayed the strongest scavenging potential with IC(50) values of (4.15 +/- 0.07, 40.32 +/- 0.09, 2.26 +/- 0.03 microM) for OH, total ROS and scavenging of ONOO(-), respectively.
Ahmad
Ijaz
I
Department of Chemistry, Kohat University of Science and Technology, Kohat 26000, NWFP, Pakistan. drijaz_chem@yahoo.com
Ahmad
Nisar
N
Wang
Fanghai
F
D000818:Animals; D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology*; D032861:Buddleja / Q000737:chemistry* / Q000145:classification; D002478:Cells, Cultured; D006027:Glycosides / Q000737:chemistry / Q000494:pharmacology*; D017665:Hydroxyl Radical / Q000037:antagonists & inhibitors; D020128:Inhibitory Concentration 50; D007668:Kidney / Q000166:cytology / Q000187:drug effects; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D015394:Molecular Structure; D010636:Phenols / Q000737:chemistry / Q000494:pharmacology*; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar
D000975:Antioxidants; D006027:Glycosides; D010636:Phenols; D010936:Plant Extracts; D017665:Hydroxyl Radical