title: Phospholipid-based ultrasonic microbubbles for loading protein and ultrasound-triggered release.
abstract: BACKGROUND
  Ultrasonic microbubbles are used as ultrasound-triggered delivery carriers for protein drugs.
  
  
  OBJECTIVE
  This work was to prepare stabilized protein-loaded phospholipid-based ultrasonic microbubbles (PUM) and to determine its value as a protein delivery system.
  
  
  METHODS
  Bovine serum albumin (BSA) was used as a model protein drug. BSA-containing PUM were prepared by dissolving lyophilized PUM powder in BSA solution. The particle size and microbubble concentration of BSA-containing PUM were measured. The BSA encapsulation efficiency as a function of BSA concentration was determined. Contrast enhancement of BSA-containing PUM in vivo was detected. The release profile of BSA from PUM was also investigated.
  
  
  RESULTS
  The mean particle size and microbubble concentration of PUM were unchanged by the presence of BSA for at least 30 minutes after preparation. The net amount of BSA entrapped in PUM was maintained unchanged with increasing BSA concentration. BSA-containing PUM were shown easily to be visible in in vivo rabbit kidney. There was no difference in echogenicity between the loaded and unloaded PUM. Ultrasound duration had a positive relationship with BSA release. Ultrasound of 30 seconds stimulated 94.1% and 93.3% of BSA release from PUM solutions containing 0.3% and 1.5% BSA, respectively.
  
  
  CONCLUSIONS
  Protein-loaded PUM exhibited satisfactory physical characteristics and were potent for using in ultrasound-triggered delivery.
authors: School of Pharmacy, Wenzhou Medical College, Zhejiang Province, China.
authors: Ying-Zheng
authors: YZ
authors: Zhao
authors: Cui-Tao
authors: CT
authors: Lu
authors: Hong-Xing
authors: HX
authors: Fu
authors: Xiao-Kun
authors: XK
authors: Li
authors: Zhi-Cai
authors: ZC
authors: Zhou
authors: Gang-Tao
authors: GT
authors: Zhao
authors: Ji-Lai
authors: JL
authors: Tian
authors: Hui-Sheng
authors: HS
authors: Gao
authors: Yi-Na
authors: YN
authors: Jiang
authors: Shu-Ping
authors: SP
authors: Hu
authors: Wei
authors: W
authors: Yang
mesh_terms: D000818:Animals; D004337:Drug Carriers; D004355:Drug Stability; D008099:Liver / Q000000981:diagnostic imaging; D008297:Male; D008970:Molecular Weight; D010316:Particle Size; D010743:Phospholipids / Q000737:chemistry*; D011506:Proteins / Q000008:administration & dosage* / Q000737:chemistry*; D011817:Rabbits; D012710:Serum Albumin, Bovine; D014465:Ultrasonics; D014463:Ultrasonography
chemical_list: D004337:Drug Carriers; D010743:Phospholipids; D011506:Proteins; D012710:Serum Albumin, Bovine