Distinct genotype and antigenicity among genogroup II sapoviruses.
SaV, a pathogen of acute gastroenteritis, is divided into five genogroups, GI to GV. However, the relation between SaV antigenicity and genetic clusters is not fully understood. We have recently identified two GII SaV strains, Mc10 and C12, which are grouped into the same cluster based on the polymerase but are grouped into distinct clusters based on the capsid. To evaluate the difference in antigenicity between these two strains, VLP were expressed in mammalian cells. An antigen ELISA demonstrated for the first time that strains in the same GII SaV genogroup, but within different clusters, have distinct antigenicities.
Oka
Tomoichiro
T
Department of Virology II, National Institute of Infectious Diseases, Gakuen 4-7-1, Tokyo 208-0011, Japan. oka-t@nih.go.jp
Miyashita
Kana
K
Katayama
Kazuhiko
K
Wakita
Takaji
T
Takeda
Naokazu
N
D000818:Animals; D000956:Antigens, Viral / Q000276:immunology*; D019556:COS Cells; D017250:Caliciviridae Infections / Q000821:virology; D002522:Chlorocebus aethiops; D004797:Enzyme-Linked Immunosorbent Assay; D005759:Gastroenteritis / Q000821:virology; D005838:Genotype; D006168:Guinea Pigs; D007106:Immune Sera / Q000276:immunology; D011817:Rabbits; D029341:Sapovirus / Q000145:classification* / Q000235:genetics* / Q000276:immunology; D014162:Transfection; D014771:Virion / Q000276:immunology* / Q000378:metabolism
D000956:Antigens, Viral; D007106:Immune Sera