title: Georgenia thermotolerans sp. nov., an actinobacterium isolated from forest soil.
abstract: A Gram-positive bacterium strain, designated TT02-04T, was isolated from a forest soil sample in Iriomote Island, Japan, and its taxonomic position was investigated by using a polyphasic approach. The peptidoglycan type of this organism was found to be A4alpha and lysine was the diagnostic cell-wall diamino acid of the peptidoglycan. The only menaquinone was MK-8(H4), and the major fatty acid was anteiso-C15:0. Galactose was detected as the cell-wall sugar. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol mannosides. The DNA G+C content was 73.0 mol%. 16S rRNA gene sequencing studies indicated that strain TT02-04T was closely related to the type strains of Georgenia ruanii (99.0%) and Georgenia muralis (97.7%). However, DNA-DNA hybridization results and phenotypic characteristics revealed that the strain differed from the currently recognized species of the genus Georgenia. Therefore, strain TT02-04T represents a novel species of the genus Georgenia, for which the name Georgenia thermotolerans sp. nov. is proposed. The type strain is TT02-04T (=NBRC 104148T=DSM 21501T).
authors: NITE Biological Resource Center (NBRC), National Institute of Technology and Evaluation, 2-5-8 Kazusakamatari, Kisarazu, Chiba 292-0818, Japan. hamada-moriyuki@nite.go.jp
authors: Moriyuki
authors: M
authors: Hamada
authors: Tomohiko
authors: T
authors: Tamura
authors: Yuumi
authors: Y
authors: Ishida
authors: Ken-ichiro
authors: K
authors: Suzuki
mesh_terms: D000192:Actinomycetales / Q000737:chemistry / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D000596:Amino Acids / Q000032:analysis; D015373:Bacterial Typing Techniques; D001482:Base Composition; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D005690:Galactose / Q000032:analysis; D007564:Japan; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010457:Peptidoglycan / Q000737:chemistry; D010743:Phospholipids / Q000032:analysis; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D014197:Trees; D024482:Vitamin K 2 / Q000032:analysis
chemical_list: D000596:Amino Acids; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D010457:Peptidoglycan; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; D005690:Galactose