title: Biodistribution of canine leucocytes labelled with technetium-99m stannous fluoride colloid in whole blood and their ability to localise to sites of induced inflammation.
abstract: This study assessed the biodistribution of autologous leucocytes radiolabelled with technetium-99m stannous fluoride colloid (99mTcSnC) for detection of foci of induced inflammation in dogs. Venous blood was collected from seven healthy dogs and incubated with 99mTcSnC for 1h at room temperature. Radiolabelled samples were injected intravenously (IV) and the dogs were scanned using a gamma camera. Another seven healthy dogs were injected intradermally with tumour necrosis factor alpha and then IV with 99mTcSnC radiolabelled autologous blood 3h later before being scanned. The radiolabelled leucocytes localised to sites of inflammation by 30 min post-injection. IV injection of autologous leucocytes radiolabelled with 99mTcSnC appears to be a sensitive method for localisation of induced foci of inflammation in dogs.
authors: Faculty of Veterinary Science, The University of Melbourne, Werribee, Victoria 3030, Australia. m.abushhiwa@pgrad.unimelb.edu.au
authors: Mohamed H
authors: MH
authors: Abushhiwa
authors: Nouria S
authors: NS
authors: Salehi
authors: Meir
authors: M
authors: Lichtenstein
authors: Peter M
authors: PM
authors: Lording
authors: Peter J
authors: PJ
authors: Finnin
authors: Robert C
authors: RC
authors: Whitton
authors: Jennifer A
authors: JA
authors: Charles
authors: Bruce W
authors: BW
authors: Parry
mesh_terms: D000818:Animals; D004283:Dog Diseases / Q000175:diagnosis / Q000000981:diagnostic imaging* / Q000378:metabolism*; D004285:Dogs; D005260:Female; D007249:Inflammation / Q000175:diagnosis / Q000000981:diagnostic imaging / Q000378:metabolism / Q000662:veterinary*; D007275:Injections, Intravenous / Q000662:veterinary; D007962:Leukocytes / Q000737:chemistry / Q000378:metabolism*; D008297:Male; D011877:Radionuclide Imaging; D017556:Technetium Compounds; D014018:Tissue Distribution; D014409:Tumor Necrosis Factor-alpha / Q000008:administration & dosage
chemical_list: D017556:Technetium Compounds; D014409:Tumor Necrosis Factor-alpha