Rapid and simple determination of ciprofloxacin resistance in clinical strains of Escherichia coli.
We recently reported a simple new in situ diffusion assay, developed as a kit, to visualize DNA fragmentation in single bacterial cells. Use of this assay in a collection of 95 genetically unrelated Escherichia coli clinical strains resulted in correct identification of all of the isolates as resistant or susceptible to ciprofloxacin, consistent with the MIC results. This relevant information is obtained in 80 min.
Santiso
Rebeca
R
INIBIC-Complejo Hospitalario Universitario Juan Canalejo, A Coruña, Spain
Tamayo
María
M
Fernández
José Luis
JL
del Carmen Fernández
María
M
Molina
Francisca
F
Villanueva
Rosa
R
Gosálvez
Jaime
J
Bou
Germán
G
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002939:Ciprofloxacin / Q000494:pharmacology*; D053938:DNA Fragmentation; D004269:DNA, Bacterial / Q000235:genetics; D024881:Drug Resistance, Bacterial; D004926:Escherichia coli / Q000187:drug effects* / Q000302:isolation & purification; D004927:Escherichia coli Infections / Q000382:microbiology*; D006801:Humans; D008826:Microbial Sensitivity Tests / Q000379:methods*; D012680:Sensitivity and Specificity
D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; D002939:Ciprofloxacin