[Application of microfluidic chip analytical systems in ABO genotyping].
Limitations of polyacrylamide gel or agarose gel electrophoretic methods in genotyping research affect the interpreting of detection results. In order to develop a simple and reliable method for appraising results of ABO genotyping detection, the microfluidic chip analysis system was established by using microfluidic chip to replace the gel electrophoresis and combining with multiplex-PCR-RFLP technique. 150 blood samples were tested by this microfluidic chip analysis system with multiplex-PCR-RFLP technique to evaluate its stability and accuracy. The results showed that all the testing results were consistent with serologic ABO genotyping results and 1 blood sample with decrease of B antigen caused by CML was identified. In conclusion, the established microfluidic chip analysis system is stable and reliable technique. Application of this technique enables the ABO genotyping results to be more objective and accurate.
Liu
Chang-Li
CL
Beijing Red Cross Blood Center, Beijing, China.
Gong
Xiao-Yan
XY
Wang
Zhuo-Yan
ZY
Ren
Fu-Rong
FR
Lü
Qiu-Shuang
QS
Miao
Tian-Hong
TH
Dai
Su-Na
SN
D000017:ABO Blood-Group System / Q000235:genetics*; D001788:Blood Grouping and Crossmatching / Q000379:methods*; D017931:DNA Primers / Q000235:genetics; D005838:Genotype; D006801:Humans; D046210:Microfluidic Analytical Techniques; D044085:Microfluidics; D020411:Oligonucleotide Array Sequence Analysis
D000017:ABO Blood-Group System; D017931:DNA Primers