title: Autoclave and beta-amylolysis lead to reduced in vitro digestibility of starch.
abstract: In this study, a combination of autoclave and beta-amylolysis was used to modulate the digestibility of normal corn starch (NCS) and wheat starch (WS). The modification procedure comprised three cycles of autoclave at 35% moisture content and 121 degrees C, beta-amylolysis, and one additional cycle of autoclave. Starch materials were sampled at each stage and characterized. The fine structure of starch was determined using high-performance size-exclusion chromatography, the micromorphology of starch dispersion was imaged using cryo-SEM, the crystalline pattern was evaluated using wide-angle X-ray powder diffraction, and the digestibility was measured using Englyst assay. After beta-amylolysis, amylose was enriched (from 25.4 to 33.2% for NCS and from 27.5 to 32.8% for WS) and the branch density was increased (from 5.2 to 7.7% for NCS and from 5.3 to 7.9% for WS). Cryo-SEM images showed that the autoclave treatment led to the formation of a low-swelling, high-density gel network, whereas beta-amylolysis nearly demolished the network structure. The loss of A-type crystalline structure and the formation of B- and V-type structures resulted from autoclave, which suggests the formation of amylose-based ordered structure. Englyst assay indicated that, due to beta-amylolysis, the resistant starch (RS) content was increased to 30 from 11% of native NCS and to 23 from 9% of native WS. In contrast, autoclave showed only minor impact on RS levels. The increase of RS observed in this study is associated with enhanced branch density, which is different from the four types of RS commonly defined.
authors: Whistler Center for Carbohydrate Research and Department of Food Science, Food Science Building, Purdue University, West Lafayette, Indiana 47907-1160, USA.
authors: B Elliot
authors: BE
authors: Hickman
authors: Srinivas
authors: S
authors: Janaswamy
authors: Yuan
authors: Y
authors: Yao
mesh_terms: D000688:Amylose / Q000737:chemistry*; D004063:Digestion; D005511:Food Handling / Q000379:methods*; D006358:Hot Temperature; D008954:Models, Biological; D014908:Triticum / Q000737:chemistry*; D014961:X-Ray Diffraction; D003313:Zea mays / Q000737:chemistry*
chemical_list: D000688:Amylose