AFM study of adsorption of protein A on a poly(dimethylsiloxane) surface.
In this paper, the morphology and kinetics of adsorption of protein A on a PDMS surface is studied by AFM. The results of effects of pH, protein concentration and contact time of the adsorption reveal that the morphology of adsorbed protein A is significantly affected by pH and adsorbed surface concentration, in which the pH away from the isoelectric point (IEP) of protein A could produce electrical repulsion to change the protein conformation, while the high adsorbed surface protein volume results in molecular networks. Protein A can form an adsorbed protein film on PDMS with a maximum volume of 2.45 x 10(-3) microm(3). This work enhances our fundamental understanding of protein A adsorption on PDMS, a frequently used substrate component in miniaturized immunoassay devices.
Yu
Ling
L
School of Chemical and Biomedical Engineering, Nanyang Technological University, Singapore.
Lu
Zhisong
Z
Gan
Ye
Y
Liu
Yingshuai
Y
Li
Chang Ming
CM
D000327:Adsorption; D004129:Dimethylpolysiloxanes / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D007526:Isoelectric Point; D018625:Microscopy, Atomic Force / Q000379:methods*; D013205:Staphylococcal Protein A / Q000737:chemistry*
D004129:Dimethylpolysiloxanes; D013205:Staphylococcal Protein A