Simultaneous determination of three antioxidants in foods and cosmetics by flow injection coupled to an ultra-short monolithic column.
The combination of an ultra-short C18 monolithic column (5 mm long) with flow injection analysis results in a versatile and efficient system that has been used for the determination of three antioxidants [propylgallate (PG), butylhydroxyanisole (BA), and butylhydroxytoluene (BT)]. Due to the wide variety of polarities of the analytes, two different carriers (carrier A: methanol-water 42% and carrier B: methanol-water 70%) were able to separate the analytes in only 85 s. The applicable concentration range, the detection, and the relative standard deviation (n=10) were: for PG, from 2.77 to 300 microg/mL, 0.84 microg/mL, 2.84%; for BA, between 1.51 and 300 microg/mL, 0.46 microg/mL, and 2.70%; and for BT, between 1.65 and 100 microg/mL, 0.55 microg/mL, and 2.22%, respectively. The method was applied and validated satisfactorily for the determination of PG, BA, and BT in food and cosmetic samples.
García-Jiménez
J F
JF
Solid Phase Spectrometry Research Group, Department of Analytical Chemistry, Campus Fuentenueva, Faculty of Sciences, University of Granada, E-18071 Granada, Spain.
Valencia
M C
MC
Capitán-Vallvey
L F
LF