title: [Acute toxicity analysis performance of CellSense biosensor with E. coli].
abstract: E. coli microbial electrodes for CellSense biosensor were prepared by polycarbonate membrane immobilization process, and their performance for heavy metals and toxic organic compounds acute toxicity determination were studied. The results showed that when E. coli was in logarithmic and stationary phase, the CellSense biosensor with E. coli showed good performance in heavy metal ions and organic pollutants acute toxicity analysis, when E. coli was in its decline phase, the stability and sensitivity of the CellSense biosensor was poor. The EC50 values of Hg2+, Cu2+, Zn2+, o-chlorophenol (2-CP) and p-nitrophenol (4-NP) detected by CellSense biosensor with E. coli were 0.6, 3.1, 5.8, 180 and 94 microg/mL, respectively. The immobilized E. coli electrodes could still suit for acute toxicity assessment after 2 months storage at 4 degrees C.
authors: State Key Laboratory of Pollution Control and Resource Reuse, College of Environmental Science and Engineering, Tongji University, Shanghai 200092, China. wangxj@mail.tongji.edu.cn
authors: Xue-Jiang
authors: XJ
authors: Wang
authors: Hong
authors: H
authors: Wang
authors: Jian-Fu
authors: JF
authors: Zhao
authors: Si-Qing
authors: SQ
authors: Xia
authors: Hong-Ning
authors: HN
authors: Zhao
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D004926:Escherichia coli / Q000235:genetics / Q000254:growth & development*; D019216:Metals, Heavy / Q000633:toxicity*; D018675:Toxicity Tests / Q000379:methods; D014874:Water Pollutants, Chemical / Q000633:toxicity*
chemical_list: D019216:Metals, Heavy; D014874:Water Pollutants, Chemical