Arsenic speciation in marine product samples: comparison of extraction-HPLC method and digestion-cryogenic trap method.
For the arsenic speciation in marine product samples, two types of pretreatment-analysis combination were compared. One is the combination of solvent extraction and high performance liquid chromatography (HPLC) followed by a highly sensitive arsenic detection, while the other is the combination of alkaline digestion and cryogenic trap (CT) method followed by a highly sensitive arsenic detection. For six certified reference materials (CRMs) of marine animal samples, the concentrations of arsenobetaine (AsB) obtained from the extraction-HPLC method were very consistent with those of trimethylated arsenic species measured by the digestion-CT method. For four seaweed samples, the determination of three arsenosugars (Sugar-1, Sugar-2, and Sugar-3) was favorably carried out by the extraction-HPLC method. Those seaweed samples were also subjected to the digestion-CT method, and the amounts of dimethylated arsenic species measured by the method were approximately equal to the sum of the amounts of dimethylarsinic acid (DMAA) and three arsenosugars (Sugar-1+Sugar-2+Sugar-3) obtained from the extraction-HPLC method.
Geng
Wenhua
W
Department of Bioengineering, Faculty of Engineering, Kagoshima University, 1-21-40 Korimoto, Kagoshima 890-0065, Japan.
Komine
Rieko
R
Ohta
Toshiharu
T
Nakajima
Tsunenori
T
Takanashi
Hirokazu
H
Ohki
Akira
A
D001149:Arsenates / Q000032:analysis; D001151:Arsenic / Q000032:analysis*; D001152:Arsenicals / Q000032:analysis; D002101:Cacodylic Acid / Q000032:analysis; D005591:Chemical Fractionation; D002851:Chromatography, High Pressure Liquid; D009005:Monosaccharides / Q000032:analysis; D012015:Reference Standards; D012624:Seaweed / Q000737:chemistry*
D001149:Arsenates; D001152:Arsenicals; D009005:Monosaccharides; C449161:arsenosugar; D002101:Cacodylic Acid; D001151:Arsenic; C038992:arsenobetaine