title: Dual-thermoresponsive phase behavior of blood compatible zwitterionic copolymers containing nonionic poly(N-isopropyl acrylamide).
abstract: Thermoresponsive statistical copolymers of zwitterionic sulfobetaine methacrylate (SBMA) and nonionic N-isopropylacrylamide (NIPAAm) were prepared with an average molecular weight of about 6.0 kDa via homogeneous free radical copolymerization. The aqueous solution properties of poly(SBMA-co-NIPAAm) were measured using a UV--visible spectrophotometer. The copolymers exhibited controllable lower and upper critical solution temperatures in aqueous solution and showed stimuli-responsive phase transition in the presence of salts. Regulated zwitterionic and nonionic molar mass ratios led to poly(SBMA-co-NIPAAm) copolymers having double-critical solution temperatures, where the water-insoluble polymer microdomains are generated by the zwitterionic copolymer region of polySBMA or nonionic copolymer region of polyNIPAAm depending on temperature. A high content of the nonionic polyNIPAAm in poly(SBMA-co-NIPAAm) exhibits nonionic aggregation at high temperatures due to the desolvation of polyNIPAAm, whereas relatively low content of polyNIPAAm in poly(SBMA-co-NIPAAm) exhibits zwitterionic aggregation at low temperatures due to the desolvation of polySBMA. Plasma protein adsorption on the surface coated with poly(SBMA-co-NIPAAm) was measured with a surface plasmon resonance (SPR) sensor. The copolymers containing polySBMA above 29 mol % showed extremely low protein adsorption and high anticoagulant activity in human blood plasma. The tunable and switchable thermoresponsive phase behavior of poly(SBMA-co-NIPAAm), as well as its high plasma protein adsorption resistance and anticoagulant activity, suggests a potential for blood-contacting applications.
authors: Department of Chemical Engineering, R&D Center for Membrane Technology, Chung Yuan Christian University, Jhong-Li, Taoyuan 320, Taiwan.
authors: Yung
authors: Y
authors: Chang
authors: Wen-Yih
authors: WY
authors: Chen
authors: Wetra
authors: W
authors: Yandi
authors: Yu-Ju
authors: YJ
authors: Shih
authors: Wan-Ling
authors: WL
authors: Chu
authors: Ying-Ling
authors: YL
authors: Liu
authors: Chih-Wei
authors: CW
authors: Chu
authors: Ruoh-Chyu
authors: RC
authors: Ruaan
authors: Akon
authors: A
authors: Higuchi
mesh_terms: D000178:Acrylamides / Q000737:chemistry*; D000925:Anticoagulants / Q000737:chemistry*; D001672:Biocompatible Materials / Q000737:chemistry*; D001798:Blood Proteins / Q000737:chemistry*; D006046:Gold / Q000737:chemistry; D006801:Humans; D044367:Phase Transition; D011108:Polymers / Q000737:chemistry*; D020349:Surface Plasmon Resonance; D013499:Surface Properties; D013696:Temperature
chemical_list: D000178:Acrylamides; D000925:Anticoagulants; D001672:Biocompatible Materials; D001798:Blood Proteins; D011108:Polymers; D006046:Gold; C067295:N-isopropylacrylamide