Suspension-Vero cell cultures as a platform for viral vaccine production.
Since Vero cells are currently considered as an acceptable cell substrate to produce a wide range of viruses, we developed a virus production platform using Vero cells adapted to grow in suspension in serum-free media. After adapting anchorage-dependent Vero cells to grow as a free-cell suspension, vesicular stomatitis virus, herpes simplex virus 1 and polio virus 1 production rates were evaluated in batch cultures using spinner flasks and perfused cultures in a bioreactor. The achieved results constitute valuable information for the development of a low-cost high-productivity process using a suspension culture of Vero cells to produce viral vaccines.
Paillet
Cristian
C
Zelltek S.A., Lisandro de la Torre 3124, Dpto. 2, (S3000CPR) Santa Fe, Pcia, Santa Fe, Argentina.
Forno
Guillermina
G
Kratje
Ricardo
R
Etcheverrigaray
Marina
M
D000818:Animals; D019149:Bioreactors; D018929:Cell Culture Techniques / Q000379:methods*; D002522:Chlorocebus aethiops; D016895:Culture Media, Serum-Free; D018259:Herpesvirus 1, Human / Q000254:growth & development; D017955:Poliovirus / Q000254:growth & development; D014709:Vero Cells; D014721:Vesicular stomatitis Indiana virus / Q000254:growth & development; D014765:Viral Vaccines / Q000096:biosynthesis*; D014776:Virus Cultivation / Q000379:methods
D016895:Culture Media, Serum-Free; D014765:Viral Vaccines