Integrated microscopy for real-time imaging of mechanotransduction studies in live cells.
Mechanical force is an important stimulus and determinant of many vascular smooth muscle cell functions including contraction, proliferation, migration, and cell attachment. Transmission of force from outside the cell through focal adhesions controls the dynamics of these adhesion sites and initiates intracellular signaling cascades that alter cellular behavior. To understand the mechanism by which living cells sense mechanical forces, and how they respond and adapt to their environment, a critical first step is to develop a new technology to investigate cellular behavior at subcellular level that integrates an atomic force microscope (AFM) with total internal reflection fluorescence (TIRF) and fast-spinning disk (FSD) confocal microscopy, providing high spatial and temporal resolution. AFM uses a nanosensor to measure the cell surface topography and can apply and measure mechanical force with high precision. TIRF microscopy is an optical imaging technique that provides high-contrast images with high z-resolution of fluorescently labeled molecules in the immediate vicinity of the cell-coverslip interface. FSD confocal microscopy allows rapid 3-D imaging throughout the cell in real time. The integrated system is broadly applicable across a wide range of molecular dynamic studies in any adherent live cells, allowing direct optical imaging of cell responses to mechanical stimulation in real time.
Trache
Andreea
A
Texas A&M Health Science Center, Cardiovascular Research Institute, College of Medicine, Department of Systems Biology and Translational Medicine, College Station, Texas 77843-1114, USA. trache@tamu.edu
Lim
Soon-Mi
SM
D000818:Animals; D018929:Cell Culture Techniques; D002468:Cell Physiological Phenomena; D002477:Cells / Q000166:cytology*; D003599:Cytoskeleton / Q000502:physiology; D004847:Epithelial Cells / Q000166:cytology; D004867:Equipment Design; D007834:Lasers; D008099:Liver / Q000166:cytology; D040542:Mechanotransduction, Cellular / Q000502:physiology*; D008853:Microscopy / Q000295:instrumentation / Q000379:methods*; D018625:Microscopy, Atomic Force / Q000295:instrumentation / Q000379:methods; D018613:Microscopy, Confocal / Q000379:methods; D008856:Microscopy, Fluorescence / Q000295:instrumentation / Q000379:methods; D018715:Microscopy, Video / Q000295:instrumentation / Q000379:methods; D051381:Rats; D012984:Software; D018511:Systems Integration; D013997:Time Factors