An effective method for hemoglobin E detection: DEAE sepharose microcolumn.
Hemoglobin E (Hb E) is a variant hemoglobin that can lead to considerable morbidity when it occurs in a compound heterozygous state with beta-thalassemia. Therefore, its detection is important because it permits antenatal counseling. Hb E is prevalent in economically weaker regions of the world. Thus, its recognition is often hindered by the high costs of sophisticated Hb E-detection assays. We developed a simple visual test based on identifying Hb E in blood samples with DEAE Sepharose microcolumn chromatography. The diagnostic accuracy of our new Hb E microcolumn assay was 100% for sensitivity, specificity, and positive and negative predictive values. Furthermore, the Hb E microcolumn assay is rapid, reproducible, and economical; hence, it offers affordable screening for Hb E in less well-equipped laboratories.
Tangvarasittichai
Orathai
O
Department of Medical Technology, Faculty of Allied Health Sciences, Naresuan University, Phitsanulok, Thailand. orathai19t@yahoo.com
Tangvarasittichai
Surapon
S
D002845:Chromatography / Q000379:methods* / Q000592:standards; D003362:Cost-Benefit Analysis; D006446:Hemoglobin E / Q000032:analysis*; D006801:Humans; D008722:Methods; D011237:Predictive Value of Tests; D012680:Sensitivity and Specificity; D012685:Sepharose / Q000031:analogs & derivatives*; D017086:beta-Thalassemia / Q000175:diagnosis*
C043982:DEAE-Sepharose CL-6B; D012685:Sepharose; D006446:Hemoglobin E