title: Treatment of metastatic melanoma with autologous Melan-A/MART-1-specific cytotoxic T lymphocyte clones.
abstract: Immunotherapy by adoptive T-cell transfer aims at maximizing tumor antigen-specific T-cell responses. We treated 14 patients at the metastatic stage in a phase II study with Melan-A-specific T-cell clones generated from patient blood. During the period required for T-cell clone generation, the patients were treated by dacarbazine. Every patient received a T-cell clone suspension followed by subcutaneous injections of interleukin 2 and interferon alpha. Patients were monitored until disease progression occurred. We succeeded in obtaining autologous Melan-A-specific cytotoxic T lymphocyte clones, which were highly reactive against tumor cells for all the patients. Of the 14 patients treated, six (43%) experienced an objective response (CR + PR) with long-term complete remission for two patients (1 CR for 5 years and 1 CR for 28 months). Furthermore, we showed that all the clinical responses were significantly associated with in vivo expansion of the Melan-A-specific T-cell repertoire. This phenomenon appeared to be significantly associated with clinical responses. Thus, over the course of an adoptive cell transfer, monitoring this melanoma-specific T-cell expansion in patient blood appears crucial for predicting the clinical efficiency of such an immunological approach.
authors: Skin Cancer Unit, Nantes CHU, Nantes, cedex 01 44093, France.
authors: Amir
authors: A
authors: Khammari
authors: Nathalie
authors: N
authors: Labarrière
authors: Virginie
authors: V
authors: Vignard
authors: Jean-Michel
authors: JM
authors: Nguyen
authors: Marie-Christine
authors: MC
authors: Pandolfino
authors: Anne C
authors: AC
authors: Knol
authors: Gaëlle
authors: G
authors: Quéreux
authors: Soraya
authors: S
authors: Saiagh
authors: Anabelle
authors: A
authors: Brocard
authors: Francine
authors: F
authors: Jotereau
authors: Brigitte
authors: B
authors: Dreno
mesh_terms: D019264:Adoptive Transfer / Q000379:methods*; D000368:Aged; D000951:Antigens, Neoplasm / Q000276:immunology*; D018414:CD8-Positive T-Lymphocytes / Q000166:cytology; D002999:Clone Cells; D005260:Female; D006801:Humans; D058965:MART-1 Antigen; D008297:Male; D008545:Melanoma / Q000276:immunology / Q000556:secondary / Q000628:therapy*; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000276:immunology*; D012878:Skin Neoplasms / Q000276:immunology / Q000473:pathology / Q000628:therapy*; D012880:Skin Pigmentation / Q000276:immunology; D013602:T-Lymphocytes, Cytotoxic / Q000166:cytology / Q000276:immunology / Q000637:transplantation*; D016896:Treatment Outcome
chemical_list: D000951:Antigens, Neoplasm; D058965:MART-1 Antigen; C548667:MLANA protein, human; D009363:Neoplasm Proteins