title: An in vivo autotransplant model of renal preservation: cold storage versus machine perfusion in the prevention of ischemia/reperfusion injury.
abstract: There is increasing proof that organ preservation by machine perfusion is able to limit ischemia/reperfusion injury in kidney transplantation. This study was designed to compare the efficiency in hypothermic organ preservation by machine perfusion or cold storage in an animal model of kidney autotransplantation. Twelve pigs underwent left nephrectomy after warm ischemic time; the organs were preserved in machine perfusion (n = 6) or cold storage (n = 6) and then autotransplanted with immediate contralateral nephrectomy. The following parameters were compared between the two groups of animals: hematological and urine indexes of renal function, blood/gas analysis values, histological features, tissue adenosine-5'-triphosphate (ATP) content, perforin gene expression in kidney biopsies, and organ weight changes were compared before and after preservation. The amount of cellular ATP was significantly higher in organs preserved by machine perfusion; moreover, the study of apoptosis induction revealed an enhanced perforin expression in the kidneys, which underwent simple hypothermic preservation compared to the machine-preserved ones. Organ weight was significantly decreased after cold storage, but it remained quite stable for machine-perfused kidneys. The present model seems to suggest that organ preservation by hypothermic machine perfusion is able to better control cellular impairment in comparison with cold storage.
authors: Nephrology, Dialysis and Renal Transplant Unit, University of Bologna, St. Orsola Hospital, Bologna, Italy. gaetano.lamanna@unibo.it
authors: Gaetano
authors: G
authors: La Manna
authors: Diletta
authors: D
authors: Conte
authors: Maria Laura
authors: ML
authors: Cappuccilli
authors: Bruno
authors: B
authors: Nardo
authors: Francesca
authors: F
authors: D'Addio
authors: Lorenza
authors: L
authors: Puviani
authors: Giorgia
authors: G
authors: Comai
authors: Francesca
authors: F
authors: Bianchi
authors: Riccardo
authors: R
authors: Bertelli
authors: Nicole
authors: N
authors: Lanci
authors: Gabriele
authors: G
authors: Donati
authors: Maria Piera
authors: MP
authors: Scolari
authors: Alessandro
authors: A
authors: Faenza
authors: Sergio
authors: S
authors: Stefoni
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D017209:Apoptosis; D003080:Cold Temperature; D005260:Female; D015870:Gene Expression; D007668:Kidney / Q000473:pathology / Q000503:physiopathology*; D016030:Kidney Transplantation; D009926:Organ Preservation / Q000295:instrumentation* / Q000379:methods*; D009929:Organ Size; D054353:Perforin / Q000235:genetics / Q000378:metabolism; D015427:Reperfusion Injury / Q000473:pathology / Q000503:physiopathology / Q000517:prevention & control*; D013552:Swine; D014182:Transplantation, Autologous
chemical_list: D054353:Perforin; D000255:Adenosine Triphosphate