Optimal self-referenced sensing using long- and short- range surface plasmons.
Dual-mode surface-plasmon resonance (SPR) sensors use both long- and short- range surface plasmon waves to differentiate surface binding interactions from interfering bulk effects. We have optimized the design of these sensors for minimum surface limit of detection (LOD) using a Cramer-Rao lower bound for spectral shift estimation. Despite trade-offs between resonance width, minimum reflectivity, and sensitivity for the two modes, a range of reasonable design parameters provides nearly optimal performance. Experimental verification using biotin-streptavidin binding as a model system reveals that sensitivity and LOD for dual-mode sensors remains competitive with single-mode sensors while compensating for bulk effects.
Hastings
J T
JT
Department of Electrical and Computer Engineering, University of Kentucky, Lexington, KY 40506, USA. hastings@engr.uky.edu
Guo
J
J
Keathley
P D
PD
Kumaresh
P B
PB
Wei
Y
Y
Law
S
S
Bachas
L G
LG
D017076:Computer-Aided Design; D004867:Equipment Design; D019544:Equipment Failure Analysis; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D020349:Surface Plasmon Resonance / Q000295:instrumentation*; D014159:Transducers