title: Volatile compounds characteristic of sinus-related bacteria and infected sinus mucus: analysis by solid-phase microextraction and gas chromatography-mass spectrometry.
abstract: Volatile compounds from human breath are a potential source of information for disease diagnosis. Breath may include volatile organic compounds (VOCs) originating in the nasal sinuses. If the sinuses are infected, disease-specific volatiles may enter exhaled air. Sinus infections are commonly caused by several known bacteria. We examined the volatiles characteristic of infectious bacteria in culture using solid-phase microextraction to collect and gas chromatography-mass spectrometry as well as gas chromatography with flame photometric detection to separate and analyze the resulting VOCs. Infected sinus mucus samples were also collected and their VOCs examined. Similar characteristic volatiles were seen from both cultures of individual "pure" bacteria and several mucus samples. However, the relative amounts of characteristic VOCs from individual bacteria differ greatly between cultures and sinus mucus. New compounds, not seen in culture were also seen in some mucus samples. Our results suggest an important role for growth substrate and environment. Our data further suggests that in some sinus mucus samples identification of bacteria-specific volatiles is possible and can suggest the identity of an infecting organism to physicians. Knowledge of these bacteria-related volatiles is necessary to create electronic nose-based, volatile-specific sensors for non-invasive examination for suspected sinus infection.
authors: Monell Chemical Senses Center, 3500 Market Street, Philadelphia, PA 19104, USA. preti@monell.org
authors: George
authors: G
authors: Preti
authors: Erica
authors: E
authors: Thaler
authors: C William
authors: CW
authors: Hanson
authors: Michelle
authors: M
authors: Troy
authors: Jason
authors: J
authors: Eades
authors: Alan
authors: A
authors: Gelperin
mesh_terms: D001419:Bacteria / Q000737:chemistry* / Q000378:metabolism; D001424:Bacterial Infections / Q000382:microbiology*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006801:Humans; D009093:Mucus / Q000737:chemistry / Q000382:microbiology*; D010256:Paranasal Sinuses / Q000737:chemistry / Q000382:microbiology*; D052617:Solid Phase Microextraction / Q000379:methods*; D055549:Volatile Organic Compounds / Q000032:analysis* / Q000378:metabolism
chemical_list: D055549:Volatile Organic Compounds