[Antiviral effects of an effective section of a prescription of traditional Chinese medicine on influenza virus A in vitro].
OBJECTIVE
To study the inhibitive effects of an effective section of a prescription of traditional Chinese medicine (TCM-ES) on influenza virus A FM1 strain in vitro.


METHODS
The experiments were performed by microcytopathic-inhibiting-assay, Neutral Red stain and inhibiting plaque-forming units (PFU) test on MDCK cell strain. By means of observing the cytopathic effects (CPE), measuring the absorbance [D(lambda)] and counting the PFU, according to Reed-Muench assay, the TCM-ES's effective dosage of 50 percentage (EC50) and treatment index (TI) to FM1 were calculated. The inhibiting dose of 50 percentage of PFU (IC50) was also figured up.


RESULTS
By CPE assay, TCM-ES'S EC50, MTC and TI to 100TCID50 FM1 strain infection were (300 +/- 18.3) mg/L, (75 +/- 6.8) mg/L and (7.1 +/- 0.7), respectively; Whereas, ribavirin's EC50, MTC and TI was (52.3 +/- 10.1) mg/L, (25 +/- 4.1) mg/L and (20.8 +/- 5.1), respectively. By Neutral Red stain assay,TCM-ES's IC50 and TI was (285.0 +/- 19.2) mg/L and (7.2 +/- 0.6), respectively; whereas ribavirin's IC50 and TI was (45.3 +/- 4. 9) mg/L and (21.2 +/- 3.1), respectively. By reducing PFU assay, the IC50 of TCM-ES and ribavirin was 300 mg/L and 50 mg/L, respectively. All the results above were almost consistent with each other (P > 0.05).


CONCLUSIONS
TCM-ES assumes antiviral action on IFV-FM1 strain in a certain degree in vitro and can rebel intracellular virus. But it is worse than the positive control medicine of ribavirin and is worthy of further study.
Huang
Sheng-Hai
SH
Department of Microbiology, Anhui Medical University, Hefei 230032, China. shhuang@ahmu.edu.cn
Feng
Xiao-Liang
XL
Zhang
Ling-Ling
LL
Xu
Shu-Ping
SP
Wu
Cheng-Yi
CY
Wei
Wei
W
D000818:Animals; D000998:Antiviral Agents / Q000302:isolation & purification / Q000494:pharmacology*; D002478:Cells, Cultured; D004285:Dogs; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000302:isolation & purification / Q000494:pharmacology*; D009980:Influenza A virus / Q000187:drug effects* / Q000502:physiology; D020128:Inhibitory Concentration 50; D010946:Plants, Medicinal / Q000737:chemistry*; D012367:RNA, Viral / Q000187:drug effects; D012254:Ribavirin / Q000494:pharmacology; D014779:Virus Replication / Q000187:drug effects
D000998:Antiviral Agents; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; D012367:RNA, Viral; D012254:Ribavirin