[Improved expression of human rotavirus G1VP7 and G3VP7 antigens in the recombinant adenoviruses by codon optimization].
OBJECTIVE
To increase the recombinant adenovirus vector mediated human rotavirus G1VP7 and G3VP7 gene expression through coden optimization.


METHODS
We have artificially synthesized two genes of group A human rotavirus that encode G1VP7 and G3VP7 according to the human biased codon. The modified genes were transfected into 293 cells using adenovirus vectors and the gene products, the respective proteins were produced. The expression level of optimized genes and wild type genes was detected by Western Blot.


RESULTS
A remarkable increase of the expression level of optimized G1VP7 and G3VP7 genes in comparison with the wild type control.


CONCLUSIONS
The coden optimization indeed help increasing the recombinant adenovirus mediated human rotavirus gene expression, which indicated the potential applicationof such recombinant adenoviruses in the development of adenoviral-vectored rotavirus vaccines.
Wang
Min
M
Institute for Viral Disease Control and Prevention, China CDC, Beijing 100052, China.
Shu
Yi
Y
Qu
Jian-guo
JG
Wang
Jian-wei
JW
Hong
Tao
T
D000256:Adenoviridae / Q000235:genetics*; D000956:Antigens, Viral / Q000235:genetics / Q000378:metabolism*; D003001:Cloning, Molecular / Q000379:methods; D003062:Codon / Q000235:genetics*; D015870:Gene Expression; D005822:Genetic Vectors / Q000235:genetics; D006801:Humans; D012401:Rotavirus / Q000235:genetics*; D014162:Transfection
D000956:Antigens, Viral; D003062:Codon