title: Cefixime-tellurite rhamnose MacConkey agar for isolation of Vero cytotoxin-producing Escherichia coli serogroup O26 from Scottish cattle and sheep faeces.
abstract: OBJECTIVE
  To compare rhamnose MacConkey agar supplemented with cefixime and tellurite (CT-RMac) and tryptone bile X-glucuronide (TBX) agars as isolation media for Vero cytotoxin-producing Escherichia coli (VTEC) serogroup O26 from animal faeces.
  
  
  RESULTS
  Nine VTEC O26 were isolated from sheep faeces; out of which six were isolated only on CT-RMac and one was isolated only on TBX. One hundred and twelve VTEC O26 were isolated from calf faeces; out of which 97% were from CT-RMac and 52% were from TBX. In a study of E. coli O26 strains, 84% of VT-positive O26 did not ferment rhamnose when compared with 16% of VT-negative O26. VT-positive (19%) and VT-negative (39%) E. coli O26 strains did not grow on CT-RMac agar.
  
  
  CONCLUSIONS
  It is important to consider that VTEC O26 strains either may ferment rhamnose or may be sensitive to the CT supplement of CT-RMac agar.
  
  
  CONCLUSIONS
  This work compares CT-RMac and TBX agars as isolation medium for VTEC O26 from Scottish animal faeces and highlights that VTEC O26 may be missed if only CT-RMac agar is used.
authors: SAC Epidemiology Research Unit, Animal Health Group, SAC Research, Edinburgh, UK. judith.evans@sac.ac.uk
authors: J
authors: J
authors: Evans
authors: H I
authors: HI
authors: Knight
authors: A W
authors: AW
authors: Smith
authors: M C
authors: MC
authors: Pearce
authors: M
authors: M
authors: Hall
authors: G
authors: G
authors: Foster
authors: J C
authors: JC
authors: Low
authors: G J
authors: GJ
authors: Gunn
mesh_terms: D000362:Agar; D000818:Animals; D002417:Cattle / Q000382:microbiology*; D020682:Cefixime / Q000494:pharmacology*; D003470:Culture Media; D005243:Feces / Q000382:microbiology*; D005285:Fermentation; D012210:Rhamnose / Q000378:metabolism*; D012756:Sheep / Q000382:microbiology*; D054323:Shiga-Toxigenic Escherichia coli / Q000302:isolation & purification* / Q000378:metabolism; D013691:Tellurium / Q000494:pharmacology*
chemical_list: D003470:Culture Media; D000362:Agar; D020682:Cefixime; C026660:tellurous acid; D013691:Tellurium; D012210:Rhamnose