The influence of liposomal adjuvant on intranasal vaccination of chickens against Newcastle disease.
The adjuvant effect of liposomes formulated with three phospholipids including phosphatidylcholine-liposomes (PC-Lip), phosphatidylserine-liposomes (PS-Lip), and stearylamine-liposomes (SA-Lip) was compared with virus alone using inactivated Newcastle disease virus (NDV) as a model antigen. The difference in adjuvanticity was evaluated using the haemagglutination-inhibition (HI) test, enzyme-linked immunosorbent assay, and a challenge study following intranasal inoculation of specific pathogen-free chickens. After two inoculations, a liposomal vaccine consisting of NDV in PC-Lip resulted in a significant increase in HI titre, up to 32-fold higher than a vaccine containing virus alone and 320-fold higher than a vaccine containing NDV in SA-Lip. PC-Lip also elicited a significant mucosal secretary immunoglobulin A response (P<0.05) in tracheal lavages and a serum IgG response (P<0.05). In response to viral challenge, all control animals died, whereas 90% of animals which received PC-Lip survived. The results suggest that PC-Lip may be suitable as an adjuvant for mucosal vaccination against NDV in chickens.
Tseng
Li-Ping
LP
Graduate Institute of Biomedical Engineering, College of Engineering, College of Medicine, National Taiwan University, Taipei, Taiwan.
Liang
Hong-Jen
HJ
Deng
Ming-Chung
MC
Lee
Kuo-Ming
KM
Pan
Ryh-Nan
RN
Yang
Jen-Chang
JC
Huang
Yi-You
YY
Liu
Der-Zen
DZ
D000276:Adjuvants, Immunologic / Q000008:administration & dosage; D000281:Administration, Intranasal; D000818:Animals; D002645:Chickens / Q000276:immunology*; D008081:Liposomes / Q000008:administration & dosage; D009521:Newcastle Disease / Q000517:prevention & control*; D009522:Newcastle disease virus / Q000276:immunology*; D011201:Poultry Diseases / Q000517:prevention & control*; D013047:Specific Pathogen-Free Organisms; D019562:Viral Load; D014765:Viral Vaccines / Q000008:administration & dosage*
D000276:Adjuvants, Immunologic; D008081:Liposomes; D014765:Viral Vaccines