title: TIP150 interacts with and targets MCAK at the microtubule plus ends.
abstract: The microtubule (MT) cytoskeleton orchestrates the cellular plasticity and dynamics that underlie morphogenesis and cell division. Growing MT plus ends have emerged as dynamic regulatory machineries in which specialized proteins-called plus-end tracking proteins (+TIPs)-bind to and control the plus-end dynamics that are essential for cell division and migration. However, the molecular mechanisms underlying the plus-end regulation by +TIPs at spindle and astral MTs have remained elusive. Here, we show that TIP150 is a new +TIP that binds to end-binding protein 1 (EB1) in vitro and co-localizes with EB1 at the MT plus ends in vivo. Suppression of EB1 eliminates the plus-end localization of TIP150. Interestingly, TIP150 also binds to mitotic centromere-associated kinesin (MCAK), an MT depolymerase that localizes to the plus end of MTs. Suppression of TIP150 diminishes the plus-end localization of MCAK. Importantly, aurora B-mediated phosphorylation disrupts the TIP150-MCAK association in vitro. We reason that TIP150 facilitates the EB1-dependent loading of MCAK onto MT plus ends and orchestrates the dynamics at the plus end of MTs.
authors: Anhui Key Laboratory for Cellular Dynamics and Chemical Biology, University of Science and Technology of China, Hefei 230027, China.
authors: Kai
authors: K
authors: Jiang
authors: Jianyu
authors: J
authors: Wang
authors: Jing
authors: J
authors: Liu
authors: Tarsha
authors: T
authors: Ward
authors: Linda
authors: L
authors: Wordeman
authors: Alec
authors: A
authors: Davidson
authors: Fengsong
authors: F
authors: Wang
authors: Xuebiao
authors: X
authors: Yao
mesh_terms: D000595:Amino Acid Sequence; D002460:Cell Line; D006367:HeLa Cells; D006801:Humans; D007150:Immunohistochemistry; D016547:Kinesins / Q000378:metabolism*; D008869:Microtubule-Associated Proteins / Q000235:genetics / Q000378:metabolism*; D008870:Microtubules / Q000378:metabolism*; D008969:Molecular Sequence Data; D011485:Protein Binding; D017386:Sequence Homology, Amino Acid; D014162:Transfection
chemical_list: C091489:KIF2C protein, human; C500985:MAPRE1 protein, human; D008869:Microtubule-Associated Proteins; D016547:Kinesins