[Application of a simple method for the detection of measles virus genome by loop-mediated isothermal amplification (LAMP)].
OBJECTIVE
A new simple RT-LAMP method was applied to detect measles virus nucleic acid and compared with nest-RT-PCR.


METHODS
Compare the detection rate of the RT-LAMP method with that of nest-RT-PCR by detecting measles virus nucleic acid from measles virus and clinical samples.


RESULTS
The nucleic acid positive rates of all 23 strains of measles virus are all 100% by the two methods. But to the detection of 18 clinical samples which are negative in measles isolation, the nest-RT-LAMP showed 56.52% positive rate of nucleic acid of measles virus and nest-RT-PCR showed 47.83%.


CONCLUSIONS
RT-LAMP is more sensitive than nest-RT-PCR.
Zhou
Jian-hui
JH
Jilin Provincial Center for Disease Control and Prevention, Changchun 130062, China.
Hou
Xiang
X
Chen
Chao
C
Wang
Shuang
S
Chang
Xin
X
Liu
Gui-yan
GY
Wang
Zhao-nan
ZN
Xu
Song-tao
ST
Ji
Yi-xin
YX
Xu
Wen-bo
WB
D016679:Genome, Viral; D006801:Humans; D008457:Measles / Q000821:virology*; D008459:Measles virus / Q000302:isolation & purification*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*