Addition of adenosine to University of Wisconsin solution: does it help?
Adenosine Triphosphate (ATP) precursors are sometimes added to preservation solutions in the belief that once the organ is reperfused, these precursors will build up ATP rapidly, returning it to its original metabolic state. This work studied ATP and metabolites during preservation of the rat liver using University of Wisconsin solution (UW), which contains adenosine, versus histidine tryptophan ketaglutarate solution a new phosphate-based preservation solution, or leeds solution (LS), which is under development at our institution (neither of the latter 2 contains adenosine). Tissue samples of perfused livers were analyzed for ATP and metabolites by high-performance liquid chromatography. UW did initially show the expected significant difference in overall adenosine levels, but the advantage had disappeared by 4 hours. At no time did UW show significantly higher levels of ATP; this was not seen following adenosine addition to LS. Only in living donor transplants where the cold ischemic time is short may there be some advantage to the addition of adenosine.
Corps
C L
CL
St James's University Teaching Hospital, Leeds, UK.
Smolenski
R
R
Potts
D
D
Pratt
J
J
Lodge
J P A
JP
D000241:Adenosine / Q000378:metabolism* / Q000627:therapeutic use; D000255:Adenosine Triphosphate / Q000378:metabolism*; D000493:Allopurinol; D000818:Animals; D001011:Aorta; D002851:Chromatography, High Pressure Liquid; D005978:Glutathione; D007328:Insulin; D007813:Laparotomy; D008099:Liver / Q000378:metabolism*; D008297:Male; D019188:Organ Preservation Solutions; D010477:Perfusion / Q000379:methods; D011887:Raffinose; D051381:Rats; D017208:Rats, Wistar
D007328:Insulin; D019188:Organ Preservation Solutions; C055206:University of Wisconsin-lactobionate solution; D000493:Allopurinol; D000255:Adenosine Triphosphate; D005978:Glutathione; D000241:Adenosine; D011887:Raffinose