Hyaluronic acid complexed to biodegradable poly L-arginine for targeted delivery of siRNAs.
BACKGROUND
Small interfering RNA (siRNA) has been recognized as a new therapeutic drug to treat various diseases by inhibition of oncogene or viral gene expression. Because hyaluronic acid (HA) has been described as a biocompatible biomaterial, we tested the nanoparticles formed by electrostatic complexation of negatively-charged HA and cationic poly L-arginine (PLR) for siRNA delivery systems.


METHODS
Different electrostatic complexes of HA and PLR (HPs) were formulated: HP101 with 50% (w/w) HA and HP110 with 9% (w/w) HA.


RESULTS
Gel retardation assays showed that HP101 and HP110 could form complexes with siRNAs. The diameters of these complexes were less than 200 nm. Cellular delivery efficiency of siRNAs by HPs depended on cell surface CD44 density. The HP-mediated delivery of siRNAs was highest in WM266.4 cells followed by B16F10 cells and COS-7 cells, in parallel with CD44 surface densities of these cell lines. TC(50) values (i.e. the HP concentrations at which 50% of cells were viable after treatment) were used as indicators of cytotoxicity. HP101 showed TC(50) values that were 2-fold and 23-fold higher than those of HP110 and PLR, respectively. After delivery into cells, siRNA exerted target-specific RNA interference effects on mRNA and protein levels. Three days after treatment of red fluorescent protein (RFP)-expressing B16F10 cells with RFP-specific siRNA complexed to HP101, cellular fluorescence signals were reduced. Intratumoral administration of RFP-specific siRNA via HP101 delivery significantly reduced the expression of RFP in tumor tissues.


CONCLUSIONS
HP101 may function as a biocompatible polymeric carrier of siRNAs and have possible application to localized siRNA delivery in vivo.
Kim
Eun-Joong
EJ
National Research Laboratory for Transporters, Targeted Drug Design, Research Institute of Pharmaceutical Sciences and Department of Pharmaceutics, College of Pharmacy, Seoul National University, Seoul, South Korea.
Shim
Gayong
G
Kim
Kwangmyeung
K
Kwon
Ick Chan
IC
Oh
Yu-Kyoung
YK
Shim
Chang-Koo
CK
D000818:Animals; D001483:Base Sequence; D001769:Blood; D002460:Cell Line; D017931:DNA Primers; D020868:Gene Silencing; D006820:Hyaluronic Acid / Q000737:chemistry*; D051379:Mice; D010316:Particle Size; D010455:Peptides / Q000737:chemistry*; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000008:administration & dosage*; D055672:Static Electricity
D017931:DNA Primers; D010455:Peptides; D034741:RNA, Small Interfering; C015462:polyarginine; D006820:Hyaluronic Acid