[Measles pathogenic surveillance from 2005 to 2007 in Guangdong Province].
OBJECTIVE
To develop pathogenic surveillance on measles and to effectively isolate measles virus. To know the genetic characterizations and molecular epidemiology of wildtype measles viruses from 2005 to 2007 in Guangdong Province, and provide the scientific basis for measles control and eradication.


METHODS
Vero/Slam cell line were used, measles viruses were isolated from throat swabs or urine specimens collected from uspected measles patients in outbreaks and sporadic patients. A 450 nucleotides fragment of the C-terminal of the nucleoprotein (N) gene was amplified and by RT-PCR and subjected to sequence and phylogenetic analysis using Bio-Edit software.


RESULTS
82 wild-type measels virus were obtained from 377 throat swabs and urine specimens from 2005 to 2007 in Guangdong Province measles lab. The measles isolation rate was 23.58% in 2005, 17.11% in 2006, 39.13% in 2007. The succeed rate of virus isolation is related to the quality of specimens collected and the days after rash occurrence.


CONCLUSIONS
We have grasped the technicalability of measles virus isolation and confirm action, and got higher isolation ratio. The wild-type measles virus isolated from Guangdong Province is of H1 genotype from 2005 to 2007, which is the same as the dominant genotype circulation.
Liu
Leng
L
Guangdong Provincial Center for Disease Prevention and control, Guangzhou 510300, China. llfest_1999@126.com
Zheng
Huan-ying
HY
Guo
Xue
X
Zhu
Jian-qiong
JQ
Ji
Yi-xin
YX
Xu
Wen-bo
WB
D002681:China / Q000453:epidemiology; D005838:Genotype; D006801:Humans; D008457:Measles / Q000175:diagnosis* / Q000453:epidemiology / Q000235:genetics; D008459:Measles virus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D017720:Molecular Epidemiology; D008969:Molecular Sequence Data; D010802:Phylogeny; D010949:Plasma / Q000821:virology; D011159:Population Surveillance / Q000379:methods*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014556:Urine / Q000821:virology