title: Relative uptake of minoxidil into appendages and stratum corneum and permeation through human skin in vitro.
abstract: We examined uptake of the model therapeutic agent, minoxidil, into appendages, stratum corneum (SC), and through human skin, under the influence of different vehicles. Quantitative estimation of therapeutic drug deposition into all three areas has not previously been reported. Finite doses of minoxidil (2%, w/v) in formulations containing varying amounts of ethanol, propylene glycol (PG), and water (60:20:20, 80:20:0, and 0:80:20 by volume, respectively) were used. Minoxidil in SC (by tape stripping), appendages (by cyanoacrylate casting), and receptor fluid was determined by liquid scintillation counting. At early times (30 min, 2 h), ethanol-containing formulations (60:20:20 and 80:20:0) caused significantly greater minoxidil retention in SC and appendages, compared to the formulation lacking ethanol (0:80:20). A significant increase in minoxidil receptor penetration occurred with the PG-rich 0:80:20 formulation after 12 h. We showed that deposition of minoxidil into appendages, SC, and skin penetration into receptor fluid were similar in magnitude. Transport by the appendageal route is likely to be a key determinant of hair growth promotion by minoxidil.
authors: School of Medicine, The University of Queensland, Princess Alexandra Hospital, Brisbane, Woolloongabba, Queensland, Australia.
authors: Jeffrey E
authors: JE
authors: Grice
authors: Susan
authors: S
authors: Ciotti
authors: Norman
authors: N
authors: Weiner
authors: Peter
authors: P
authors: Lockwood
authors: Sheree E
authors: SE
authors: Cross
authors: Michael S
authors: MS
authors: Roberts
mesh_terms: D002626:Chemistry, Pharmaceutical; D003487:Cyanoacrylates / Q000737:chemistry; D015194:Diffusion Chambers, Culture; D018859:Hair Follicle / Q000378:metabolism; D006801:Humans; D066298:In Vitro Techniques; D008914:Minoxidil / Q000008:administration & dosage / Q000493:pharmacokinetics*; D010539:Permeability; D012869:Skin Absorption; D014665:Vasodilator Agents / Q000008:administration & dosage / Q000493:pharmacokinetics*; D014867:Water / Q000737:chemistry
chemical_list: D003487:Cyanoacrylates; D014665:Vasodilator Agents; D014867:Water; D008914:Minoxidil