title: Density transfer as a method to analyze the progression of DNA replication forks.
abstract: The density transfer technique is a valuable tool to examine the progression of individual DNA replication forks. It is based on the transfer of cells from a medium containing dense isotopes to a medium with light (normal) isotopes (or vice versa), to obtain DNA sequences hybrid in density that can be identified as replicated molecules. Using specific DNA probes along a chromosome, the dense isotope transfer method allows determining the extent of replication at any position of a replicon and the rate of replication fork progression. In the eukaryotic model budding yeast, this technique has been useful to establish a role for different proteins during the elongation of chromosomal replication and to analyze the movement and stability of DNA replication forks under different experimental conditions.
authors: Centro de Biología Molecular Severo Ochoa, CSIC/UAM, Cantoblanco, Madrid, Spain.
authors: José Antonio
authors: JA
authors: Tercero
mesh_terms: D002247:Carbon Isotopes; D002499:Centrifugation, Density Gradient; D015825:Chromosomes, Fungal / Q000378:metabolism; D003470:Culture Media; D015342:DNA Probes; D004261:DNA Replication; D004271:DNA, Fungal / Q000096:biosynthesis* / Q000235:genetics / Q000302:isolation & purification*; D009587:Nitrogen Isotopes; D009693:Nucleic Acid Hybridization; D012093:Replicon; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism
chemical_list: D002247:Carbon Isotopes; D003470:Culture Media; D015342:DNA Probes; D004271:DNA, Fungal; D009587:Nitrogen Isotopes; D029701:Saccharomyces cerevisiae Proteins