Developments in fluorescent probes for receptor research.
Early reports on the identification of fluorescent probes for receptors date back to mid-1970s. Fluorescent probes were initially used to visualize molecular targets in an analogous way to the use of fluorescent antibodies but with the same resolution as isotopically labelled ligands. In parallel to the rapid development of techniques, such as fluorescence correlation spectroscopy, multi-photon excitation fluorescence microscopy, fluorescence polarization and in vivo fluorescence imaging, fluorescent probes are becoming multifaceted tools in life science. The present review will focus on how the design of fluorescent ligands for receptors has evolved to meet the needs of most recent fluorescence applications.
Leopoldo
Marcello
M
Dipartimento Farmaco-Chimico, Università degli Studi di Bari, via Orabona, 4, 70125, Bari, Italy. leopoldo@farmchim.uniba.it
Lacivita
Enza
E
Berardi
Francesco
F
Perrone
Roberto
R
D000818:Animals; D035843:Biomedical Research / Q000379:methods / Q000639:trends*; D055808:Drug Discovery / Q000379:methods / Q000639:trends; D005454:Fluorescence Polarization / Q000379:methods; D005456:Fluorescent Dyes / Q000138:chemical synthesis*; D006801:Humans; D011956:Receptors, Cell Surface / Q000032:analysis*; D013050:Spectrometry, Fluorescence / Q000379:methods
D005456:Fluorescent Dyes; D011956:Receptors, Cell Surface