title: A randomly integrated transgenic H19 imprinting control region acquires methylation imprinting independently of its establishment in germ cells.
abstract: The imprinted expression of the mouse Igf2/H19 locus is governed by the differential methylation of the imprinting control region (ICR), which is established initially in germ cells and subsequently maintained in somatic cells, depending on its parental origin. By grafting a 2.9-kbp H19 ICR fragment into a human beta-globin yeast artificial chromosome in transgenic mice, we previously showed that the ICR could recapitulate imprinted methylation and expression at a heterologous locus, suggesting that the H19 ICR in the beta-globin locus contained sufficient information to maintain the methylation mark (K. Tanimoto, M. Shimotsuma, H. Matsuzaki, A. Omori, J. Bungert, J. D. Engel, and A. Fukamizu, Proc. Natl. Acad. Sci. USA 102:10250-10255, 2005). Curiously, however, the transgenic H19 ICR was not methylated in sperm, which was distinct from that seen in the endogenous locus. Here, we reevaluated the ability of the H19 ICR to mark the parental origin using more rigid criteria. In the testis, the methylation levels of the solitary 2.9-kbp transgenic ICR fragment varied significantly between six transgenic mouse lines. However, in somatic cells, the paternally inherited ICR fragment exhibited consistently higher methylation levels at five out of six randomly integrated sites in the mouse genome. These results clearly demonstrated that the H19 ICR could acquire parent-of-origin-dependent methylation after fertilization independently of the chromosomal integration site or the prerequisite methylation acquisition in male germ cells.
authors: Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Ibaraki, Japan.
authors: Hitomi
authors: H
authors: Matsuzaki
authors: Eiichi
authors: E
authors: Okamura
authors: Motoshi
authors: M
authors: Shimotsuma
authors: Akiyoshi
authors: A
authors: Fukamizu
authors: Keiji
authors: K
authors: Tanimoto
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D019175:DNA Methylation; D005260:Female; D018392:Genomic Imprinting; D005854:Germ Cells / Q000502:physiology*; D006801:Humans; D007335:Insulin-Like Growth Factor II / Q000235:genetics*; D019901:Locus Control Region; D008297:Male; D051379:Mice; D008822:Mice, Transgenic; D010375:Pedigree; D062085:RNA, Long Noncoding; D022661:RNA, Untranslated / Q000235:genetics*; D013737:Testis / Q000166:cytology / Q000502:physiology; D019076:Transgenes
chemical_list: C094335:H19 long non-coding RNA; C513121:IGF2 protein, mouse; D062085:RNA, Long Noncoding; D022661:RNA, Untranslated; D007335:Insulin-Like Growth Factor II