title: Melatonin plus porcine bone on discrete calcium deposit implant surface stimulates osteointegration in dental implants.
abstract: The aim of this study was to evaluate the effect of the topical application of melatonin mixed with collagenized porcine bone to accelerate the osteointegration on the rough discrete calcium deposit (DCD) surface implants in Beagle dogs 3 months after their insertion. In preparation for subsequent insertion of dental implants, lower premolars and molars were extracted from 12 Beagle dogs. Each mandible received three parallel wall implants with discrete calcium deposit (DCD) surface of 4 mm in diameter and 10 mm in length. The implants were randomly assigned to the distal sites on each side of the mandible in three groups: group I implants alone, group II implants with melatonin and group III implants with melatonin and porcine bone. Prior to implanting, 5 mg lyophylized powdered melatonin was applied to one bone hole at each side of the mandible. None was applied at the control sites. Ten histological sections per implant were obtained for histomorphometric studies. After a 4-wk treatment period, melatonin significantly increased the perimeter of bone that was in direct contact with the treated implants (P < 0.0001), bone density (P < 0.0001), new bone formation (P < 0.0001) in comparison with control implants. Topical application of melatonin on DCD surface may act as a biomimetic agent in the placement of endo-osseous dental implants and enhance the osteointegration. Melatonin combined with porcine bone on DCD implants reveals more bone to implant contact at 12 wk (84.5 +/- 1.5%) compared with melatonin treated (75.1 +/- 1.4%) and nonmelatonin treated surface implants (64 +/- 1.4%).
authors: Department of General and Implant Dentistry, Faculty of Medicine and Dentistry, University of Murcia, Murcia, Spain.
authors: José Luis
authors: JL
authors: Calvo-Guirado
authors: Department of Special Care in Dentistry, School of Dentistry, University of Granada, Granada, Spain.
authors: Gerardo
authors: G
authors: Gómez-Moreno
authors: Department of Oral Surgery, Faculty of Medicine, University of Genova, Genova, Italy.
authors: Antonio
authors: A
authors: Barone
authors: Department of Special Care in Dentistry, School of Dentistry, University of Granada, Granada, Spain.
authors: Antonio
authors: A
authors: Cutando
authors: Department of Radiology, Faculty of Medicine and Dentistry, University of Murcia, Murcia, Spain.
authors: Miguel
authors: M
authors: Alcaraz-Baños
authors: Department of Restorative Dentistry, Faculty of Medicine and Dentistry, University of Murcia, Murcia, Spain.
authors: Fernando
authors: F
authors: Chiva
authors: Department of General and Implant Dentistry, Faculty of Medicine and Dentistry, University of Murcia, Murcia, Spain.
authors: Laura
authors: L
authors: López-Marí
authors: Department of Special Care in Dentistry, School of Dentistry, University of Granada, Granada, Spain.
authors: Javier
authors: J
authors: Guardia
mesh_terms: D000818:Animals; D001842:Bone and Bones; D002118:Calcium / Q000494:pharmacology*; D015921:Dental Implants; D004285:Dogs; D006651:Histocytochemistry; D008297:Male; D008550:Melatonin / Q000494:pharmacology*; D016348:Osseointegration / Q000187:drug effects*; D023861:Photography, Dental; D013552:Swine
chemical_list: D015921:Dental Implants; D008550:Melatonin; D002118:Calcium