title: Epidemiology and phylogenetic analysis of crimean-congo hemorrhagic fever viruses in xinjiang, china.
abstract: In 2004 and 2005, an epidemiological survey of Crimean-Congo hemorrhagic fever virus (CCHFV) was conducted in Xinjiang, China. A total of 5,629 serum samples of human and livestock were collected and tested for the CCHFV antibody, and 17,319 ticks were collected for viral identification. Reverse passive hemagglutination inhibition assays showed that the average prevalence of CCHFV antibody was 1.7% for the humans and 12.7% for the livestock. A relatively high antibody prevalence, ranging from 19.1% to 23.4%, was found in the livestock of the northwest, southwest, and northeast parts of the Tarim Basin. When the ticks were pooled to inoculate suckling mice, followed by reverse transcription-PCR (RT-PCR) to detect CCHFV RNA, the average RT-PCR-positive rates for Hyalomma asiaticum kozlovi and H. asiaticum asiaticum were 12.9% and 2.6%, respectively. A significant correlation was found between the antibody prevalence in the livestock and the CCHFV prevalence in H. asiaticum of the same geographic region. No CCHFV RNA was detected in Dermacentor nivenus, Rhipicephalus turanius, or Rhipicephalus sanguineus. A total of 27 partial S segments of CCHFVs were sequenced and used for phylogeny analysis. All but one Chinese isolate grouped into the Asia 1 clade, which contains the strains from Xinjiang and Uzbekistan, while the other strain, Fub90009, grouped with strains from the Middle East.
authors: Xinjiang University, Urmuqi, China.
authors: Surong
authors: S
authors: Sun
authors: Xiang
authors: X
authors: Dai
authors: Muhetaer
authors: M
authors: Aishan
authors: Xinhui
authors: X
authors: Wang
authors: Weiwei
authors: W
authors: Meng
authors: Conghui
authors: C
authors: Feng
authors: Fuchun
authors: F
authors: Zhang
authors: Changshou
authors: C
authors: Hang
authors: Zhihong
authors: Z
authors: Hu
authors: Yujiang
authors: Y
authors: Zhang
mesh_terms: D000818:Animals; D000829:Animals, Domestic / Q000276:immunology; D000914:Antibodies, Viral / Q000097:blood; D002681:China / Q000453:epidemiology; D016000:Cluster Analysis; D006385:Hemagglutination Inhibition Tests / Q000379:methods; D003225:Hemorrhagic Fever Virus, Crimean-Congo / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D006479:Hemorrhagic Fever, Crimean / Q000453:epidemiology* / Q000662:veterinary* / Q000821:virology; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D016036:Seroepidemiologic Studies; D044967:Serum / Q000276:immunology; D013987:Ticks / Q000821:virology
chemical_list: D000914:Antibodies, Viral