A closed concept of extractive whole cell microbial transformation of benzaldehyde into L-phenylacetylcarbinol by Saccharomyces cerevisiae in novel polyethylene-glycol-induced cloud-point system.
Extractive microbial transformation of benzaldehyde into L-phenylacetylcarbinol (L-PAC) by Saccharomyces cerevisiae (Baker's yeast) has been carried out in a novel polyethylene-glycol-induced cloud-point system (PEG-CPS). The extractive microbial transformation in the PEG-CPS and a downstream process for stripping of the product from the microbial transformation broth with microemulsion extraction are demonstrated. The results indicate that the PEG-CPS maintains the advantage of CPS for in situ extraction of polar product in the microbial transformation. At the same time, the utilization of hydrophilic nonionic surfactant in the PEG-CPS is favorable for stripping of product from the nonionic surfactant in the microbial transformation broth by Winsor I microemulsion extraction. Thus, a closed concept of in situ extraction of polar product in microbial transformation and its downstream process of product recovery are fulfilled at the same time.
Wang
Zhilong
Z
School of Pharmacy, Shanghai Jiao Tong University, Shanghai, 200240, People's Republic of China. zlwang@sjtu.edu.cn
Liang
Rui
R
Xu
Jian-He
JH
Liu
Yubo
Y
Qi
Hanshi
H
D000096:Acetone / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism; D001547:Benzaldehydes / Q000737:chemistry / Q000378:metabolism*; D001709:Biotechnology / Q000379:methods*; D001711:Biotransformation / Q000187:drug effects; D004655:Emulsions; D044367:Phase Transition / Q000187:drug effects; D011092:Polyethylene Glycols / Q000494:pharmacology*; D012441:Saccharomyces cerevisiae / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism*; D012997:Solvents; D013501:Surface-Active Agents / Q000494:pharmacology; D013696:Temperature
D001547:Benzaldehydes; D004655:Emulsions; D012997:Solvents; D013501:Surface-Active Agents; D000096:Acetone; D011092:Polyethylene Glycols; C035293:1-hydroxy-1-phenyl-2-propanone; C032175:benzaldehyde