Antioxidative effect and active components from leaves of Lotus ( Nelumbo nucifera ).
The DPPH scavenging effect, the inhibition of human low-density lipoprotein oxidation, and antioxidative contents were employed for the activity-guided purification to identify the antioxidant components of lotus leaves (leaves of Nelumbo nucifera Gaertn.). The methanolic extract of lotus leaves (LLM) was separated into ethyl acetate (LLME), n-butanol (LLMB), and water (LLMW) fractions. LLME and LLMB exhibited greater capacity to scavenge DPPH radical, delayed LDL oxidation, and had higher antioxidative contents than LLMW. Seven flavonoids were isolated from both fractions by column chromatography. On the basis of 1D- and 2D-NMR experiments and MS data analyses, these compounds were identified as catechin (1), quercetin (2), quercetin-3-O-glucopyranoside (3), quercetin-3-O-glucuronide (4), quercetin-3-O-galactopyranoside (5), kaempferol-3-O-glucopyranoside (6), and myricetin-3-O-glucopyranoside (7). Quercetin and its glycosides (compounds 2-5) exerted potent inhibition of LDL oxidation, whereas myricetin-3-O-glucopyranoside (7) showed stronger DPPH scavenging activity. These results indicate that the antioxidant capacity of lotus leaves is partially relevant to its flavonoids.
Lin
Hong-Yu
HY
Graduate Institute of Food Science and Technology, Center for Food and Biomolecules, College of Bioresources and Agriculture, National Taiwan University, Taipei 106, Taiwan.
Kuo
Yueh-Hsiung
YH
Lin
Yun-Lian
YL
Chiang
Wenchang
W
D000975:Antioxidants / Q000737:chemistry* / Q000494:pharmacology; D001769:Blood / Q000378:metabolism; D006801:Humans; D015227:Lipid Peroxidation / Q000187:drug effects; D008077:Lipoproteins, LDL / Q000737:chemistry / Q000378:metabolism; D008297:Male; D031653:Nelumbo / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry* / Q000494:pharmacology; D018515:Plant Leaves / Q000737:chemistry*
D000975:Antioxidants; D008077:Lipoproteins, LDL; D010936:Plant Extracts