Label-free optical imaging of mitochondria in live cells.
The far-field optical imaging of mitochondria of live cells without the use of any label is demonstrated. It uses a highly sensitive photothermal method and has a resolution comparable to confocal fluorescence setups. The morphological states of mitochondria were followed under different physiological treatments, and the role of cytochrome c was ruled out as the main origin of the photothermal signals. This label free optical method provides a high contrast imaging of live mitochondria and should find many applications in biosciences.
Lasne
David
D
Blab
Gerhard A
GA
De Giorgi
Francesca
F
Ichas
François
F
Lounis
Brahim
B
Cognet
Laurent
L