title: [Investigation on induced expansion of erythroid cells from cord blood CD34(+) cells in vitro].
abstract: This study was aimed to investigate a beneficial approach for resolving the deficiency of blood source, preventing the infection resulting from blood transfusion and overcoming the knotty match of patients with rare blood group by using massive expansion of erythroid cells from cord blood CD34(+) cells in vitro. The CD34(+) cells from human cord blood were cultured in serum-free medium supplemented with stem cell factor (SCF), interleukin-3 (IL-3) and erythropoietin (EPO) for 1 week, then expansion and differentiation of CD34(+) cells into erythroid cells were supported by co-culture with human mesenchymal stem cells (MSCs) derived from bone marrow for 2 weeks. The results indicated that after culture for 23 days, the expansion multiple of total cell number reached 2.52 x 10(5), and over 95% of these cells were erythroid cells as compared with less than 1% of myelomonocytic (CD14(+) or CD15(+)) cells and megakaryocytic (CD41(+)) cells. However, the culture system without MSC support was significantly disadvantaged both in expansion ability and ratio of erythroid cells when compared with MSC supporting system. It is concluded that the erythroid cells can be produced from CD34(+) cells in large scale by culturing in the system comprised of cytokine sets and MSC feeders, in which MSCs can support the proliferation and differentiation of erythroid cells.
authors: Beijing Red Cross Blood Center, Beijing, China.
authors: Yan-Jun
authors: YJ
authors: Jia
authors: Jiang
authors: J
authors: Liu
authors: Ke-Ying
authors: KY
authors: Zhang
authors: Xiao-Yan
authors: XY
authors: Shan
authors: Wei
authors: W
authors: Li
authors: Xiao-Mei
authors: XM
authors: He
authors: Li-Jun
authors: LJ
authors: Wang
authors: Na
authors: N
authors: Liu
authors: Lin
authors: L
authors: Wang
authors: Shuang
authors: S
authors: Cui
authors: Lei
authors: L
authors: Ni
authors: Bo-Tao
authors: BT
authors: Zhao
authors: Zhi-Yin
authors: ZY
authors: Gong
authors: Dong-Mei
authors: DM
authors: Wang
authors: Song-Ming
authors: SM
authors: Gao
authors: Zhi-Xin
authors: ZX
authors: Zhang
mesh_terms: D018952:Antigens, CD34; D018929:Cell Culture Techniques / Q000379:methods*; D002454:Cell Differentiation; D005312:Fetal Blood / Q000166:cytology*; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D006801:Humans
chemical_list: D018952:Antigens, CD34