title: [In vitro study of directional inducible differentiation of inner ear hair cells from human adipose-derived mesenchymal stem cells].
abstract: OBJECTIVE
  To explore the feasibility of directionally inducing human adipose-derived mesenchymal stem cells (hAD-MSCs) towards inner ear hair cells.
  
  
  METHODS
  Mesenchymal stem cells from human adipose tissue were isolated, purified and cultured in vitro. hAD-MSCs were induced to neural stem/progenitor-like cell, and then co-cultured with embryonic chick otic vesicle cells. Processed hAD-MSCs were tested by immunostaining to ascertain whether they expressed characteristic hair cell markers.
  
  
  RESULTS
  Morphologically, hAD-MSCs were induced to differentiate into neural stem/progenitor cells and expressed specific neural markers. After being co-cultured with embryonic chick otic vesicle cells, hAD-MSCs expressed specific surface markers of inner ear hair cells.
  
  
  CONCLUSIONS
  hAD-MSCs can be directionally induced to differentiate towards hair cell-like cells in vitro.
authors: Department of Otorhinolaryngoogy, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100730, China.
authors: Xian-dao
authors: XD
authors: Yuan
authors: Xi
authors: X
authors: Yan
authors: Hua
authors: H
authors: Yang
authors: Zhi-qiang
authors: ZQ
authors: Gao
authors: Kang-hua
authors: KH
authors: Li
authors: Qin
authors: Q
authors: Han
authors: Shan
authors: S
authors: Lu
authors: Xiao-wei
authors: XW
authors: Chen
authors: Hong
authors: H
authors: Jiang
authors: Chun-hua
authors: CH
authors: Zhao
mesh_terms: D000273:Adipose Tissue / Q000166:cytology; D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods*; D002454:Cell Differentiation; D002478:Cells, Cultured; D002642:Chick Embryo; D018920:Coculture Techniques; D006199:Hair Cells, Auditory, Inner / Q000166:cytology*; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology*