Effects of hydroxylpropyl-β-cyclodextrin on in vitro insulin stability.
The objective of this study was to elucidate the effects of hydroxylpropyl-beta-cyclodextrin (HP-beta-CD) on the in vitro stability of insulin. It was found that HP-beta-CD had positive effects on the stability of insulin in acid and base and under high temperature conditions. Furthermore, use of HP-beta-CD could also increase the stability of disulfide bonds which are important to the conformation of insulin. Through (1)H-NMR experiments it was found that the protective effect of HP-beta-CD was due to complexation with insulin. The results suggest that the presence of HP-beta-CD could improve the stability of insulin in different environments.
Zhang
Liefeng
L
Jiangsu Key Laboratory for Supramolecular Medicinal and Applications, Nanjing Normal University, Nanjing 210097, P.R. China.
Zhu
Wenjie
W
Jiangsu Key Laboratory for Supramolecular Medicinal and Applications, Nanjing Normal University, Nanjing 210097, P.R. China.
Song
Lingling
L
Jiangsu Key Laboratory for Supramolecular Medicinal and Applications, Nanjing Normal University, Nanjing 210097, P.R. China.
Wang
Yifan
Y
Jiangsu Key Laboratory for Supramolecular Medicinal and Applications, Nanjing Normal University, Nanjing 210097, P.R. China.
Jiang
Hui
H
Jiangsu Key Laboratory for Supramolecular Medicinal and Applications, Nanjing Normal University, Nanjing 210097, P.R. China.
Xian
Suyun
S
Jiangsu Key Laboratory for Supramolecular Medicinal and Applications, Nanjing Normal University, Nanjing 210097, P.R. China.
Ren
Yong
Y
Jiangsu Key Laboratory for Supramolecular Medicinal and Applications, Nanjing Normal University, Nanjing 210097, P.R. China.
D000073738:2-Hydroxypropyl-beta-cyclodextrin; D003505:Cyclodextrins / Q000737:chemistry / Q000378:metabolism*; D004355:Drug Stability; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D007328:Insulin / Q000378:metabolism*; D004364:Pharmaceutical Preparations / Q000737:chemistry / Q000378:metabolism*; D047392:beta-Cyclodextrins / Q000737:chemistry / Q000378:metabolism*
D003505:Cyclodextrins; D007328:Insulin; D004364:Pharmaceutical Preparations; D047392:beta-Cyclodextrins; D000073738:2-Hydroxypropyl-beta-cyclodextrin