title: Isolation and characterization of Xenorhabdus nematophila transposon insertion mutants defective in lipase activity against Tween.
abstract: We identified Xenorhabdus nematophila transposon mutants with defects in lipase activity. One of the mutations, in yigL, a conserved gene of unknown function, resulted in attenuated virulence against Manduca sexta insects. We discuss possible connections between lipase production, YigL, and specific metabolic pathways.
authors: Department of Bacteriology, University of Wisconsin-Madison, Madison, Wisconsin, USA.
authors: Gregory R
authors: GR
authors: Richards
authors: Eugenio I
authors: EI
authors: Vivas
authors: Aaron W
authors: AW
authors: Andersen
authors: Delmarie
authors: D
authors: Rivera-Santos
authors: Sara
authors: S
authors: Gilmore
authors: Garret
authors: G
authors: Suen
authors: Heidi
authors: H
authors: Goodrich-Blair
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics / Q000502:physiology*; D004251:DNA Transposable Elements / Q000235:genetics / Q000502:physiology*; D008049:Lipase / Q000235:genetics / Q000502:physiology*; D018402:Manduca / Q000382:microbiology*; D005810:Multigene Family / Q000235:genetics / Q000502:physiology; D016254:Mutagenesis, Insertional / Q000379:methods*; D016366:Open Reading Frames / Q000235:genetics / Q000502:physiology; D014774:Virulence / Q000235:genetics; D020593:Xenorhabdus / Q000201:enzymology* / Q000235:genetics / Q000472:pathogenicity*
chemical_list: D001426:Bacterial Proteins; D004251:DNA Transposable Elements; D008049:Lipase