Comparison of two full automatic synthesis methods of 9-(4-[(18)F]fluoro-3-hydroxymethylbutyl)guanine using different chemistry modules.
We have developed synthesis methods for 9-(4-[(18)F]fluoro-3-hydroxymethylbutyl)guanine ([(18)F]FHBG) using two commercial automatic chemistry modules, Tracerlab MX and Explora RN, and compared radiochemical yields. Synthesis conditions and sequence programs were modified for two modules because both these modules have different mechanical structures, including heater type, vacuum system, reactor, and tubing size. Synthesis using the Tracerlab MX module showed a 21.0+/-3.8% yield of radiochemical, which was 98+/-0.9% pure; the total preparation time was 63.0+/-5.0min including an HPLC purification step. In contrast, synthesis using the Explora RN module showed a 32.0+/-1.2% yield of radiochemical, which was 99.0+/-0.6% pure; the total preparation time was 38+/-2min, using different HPLC purification conditions and without the HPLC solvent evaporation step.
Kang
Se Hun
SH
Molecular Imaging and Therapy Branch, National Cancer Center, Goyang, Republic of Korea.
Oh
Seung Jun
SJ
Lee
Sang Ju
SJ
Kang
Keon Wook
KW
Kim
Seok-ki
SK
Lee
Ho-young
HY
Lee
Sang Jin
SJ
Kim
In Hoo
IH
Lee
Won Koo
WK
D001331:Automation / Q000295:instrumentation; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005462:Fluorine Radioisotopes; D006147:Guanine / Q000031:analogs & derivatives* / Q000138:chemical synthesis; D007553:Isotope Labeling / Q000379:methods*; D011786:Quality Control; D011854:Radiochemistry / Q000295:instrumentation; D019275:Radiopharmaceuticals / Q000138:chemical synthesis*
C113308:9-(4-fluoro-3-hydroxymethylbutyl)guanine; D005462:Fluorine Radioisotopes; D019275:Radiopharmaceuticals; D006147:Guanine