title: Ferromagnetic levan composite: an affinity matrix to purify lectin.
abstract: A simple and inexpensive procedure used magnetite and levan to synthesize a composite recovered by a magnetic field. Lectins from Canavalia ensiformis (Con A) and Cratylia mollis (Cramoll 1 and Cramoll 1, 4) did bind specifically to composite. The magnetic property of derivative favored washing out contaminating proteins and recovery of pure lectins with glucose elution. Cramoll 1 was purified by this affinity binding procedure in two steps instead of a previous three-step protocol with ammonium sulfate fractionation, affinity chromatography on Sephadex G-75, and ion exchange chromatography through a CM-cellulose column.
authors: Laboratório de Glicoproteínas, Departamento de Bioquímica, Universidade Federal de Pernambuco, 50670-901 Recife, PE, Brazil.
authors: Renata
authors: R
authors: Angeli
authors: Nathalia V N
authors: NV
authors: da Paz
authors: Jackeline C
authors: JC
authors: Maciel
authors: Flávia F B
authors: FF
authors: Araújo
authors: Patrícia M G
authors: PM
authors: Paiva
authors: Glícia M T
authors: GM
authors: Calazans
authors: Ana Paula
authors: AP
authors: Valente
authors: Fábio C L
authors: FC
authors: Almeida
authors: Luana C B B
authors: LC
authors: Coelho
authors: Luiz B
authors: LB
authors: Carvalho
authors: Maria da Paz C
authors: Mda P
authors: Silva
authors: Maria Tereza
authors: MT
authors: dos Santos Correia
mesh_terms: D032449:Canavalia / Q000737:chemistry; D002853:Chromatography, Liquid; D003208:Concanavalin A / Q000302:isolation & purification; D007887:Fabaceae / Q000737:chemistry; D052203:Ferrosoferric Oxide / Q000737:chemistry*; D005630:Fructans / Q000737:chemistry* / Q000378:metabolism; D006385:Hemagglutination Inhibition Tests; D019906:Nuclear Magnetic Resonance, Biomolecular; D037121:Plant Lectins / Q000302:isolation & purification* / Q000378:metabolism; D017355:Zymomonas / Q000737:chemistry
chemical_list: D005630:Fructans; D037121:Plant Lectins; D003208:Concanavalin A; C072599:levan; D052203:Ferrosoferric Oxide