[Study of the clinical and laboratory features of hand-foot-mouth disease].
OBJECTIVE
To study the clinical and laboratory features of the mild and severe hand-foot-mouth diseases (HFMD) in Shenzhen in 2008.


METHODS
145 cases were observed in East-Lake Hospital and Shenzhen Children's Hospital. Of the 145 cases, 124 mild cases and 21 severe cases were involved.All the clinical data and laboratory findings were collected and summarized. After collection of the acute and convalescent consecutive stools and peripheral bloods from the patients with HFMDI, EV71 genes were amplified from these samples by RT-PCR. Enterovirus 71 were cultured and isolated using Vero cell line and R&D cell line.


RESULTS
The WBC counts and blood glucose levels of the severe cases were significantly elevated, but the ages of the severe ones significantly decreased compared with those of the mild cases (P < 0.05). EV71 genes could be detected by RT-PCR with 35% positive rate in mild cases and 67% in severe cases. The EV71 gene detection rate of the severe cases was significantly increased in contrast to that of the mild ones. The EV71 were isolated and cultured from the stools of 9 patients, one specimens from the dead's stool. Two severe cases died of neurogenic pulmonary edema and brain-stem encephalitis.


CONCLUSIONS
EV71 mainly contributes to HFMD and is responsible for death of some severe cases. High fever, less rash, elevated white blood cell counts and blood glucose concentrations as well as age less than 4 years old should be used for prediction of severe cases.
Liu
Ying-xia
YX
Shenzhen East-Lake Hospital, Children's Hospital, Shenzhen 518020, China.
Xie
Jing-jing
JJ
He
Yan-xia
YX
Liu
Wei-long
WL
Zhang
Ming-xia
MX
Le
Xiao-hua
XH
Fu
Dan
D
Chen
Xin-chun
XC
Yang
Gui-lin
GL
Cao
De-zhi
DZ
Zhang
Hong-mei
HM
Xu
Liu-me
LM
Zhu
Xiu-yun
XY
Tan
Ya
Y
Yang
Qian-ting
QT
Zhou
Bo-ping
BP
D000328:Adult; D001786:Blood Glucose / Q000502:physiology*; D002648:Child; D004770:Enterovirus / Q000302:isolation & purification*; D004769:Enterovirus Infections / Q000097:blood* / Q000473:pathology; D005260:Female; D006232:Hand, Foot and Mouth Disease / Q000097:blood* / Q000473:pathology / Q000821:virology; D006801:Humans; D007753:Laboratories; D007958:Leukocyte Count / Q000706:statistics & numerical data*; D008297:Male; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012720:Severity of Illness Index
D001786:Blood Glucose