Association between MGMT promoter hypermethylation and p53 mutation in glioblastoma.
O(6)-methylguanine-DNA methyltransferase (MGMT) is a DNA repair enzyme that removes alkyl groups from the O(6) position of guanine. MGMT is transcriptionally silenced by promoter hypermethylation in several human neoplasia. We used methylation-specific PCR (MSP) to analyze the MGMT promoter methylation status of 50 glioblastoma tumors. Hypermethylation was detected in 24 of 50 (48%) samples. We also analyzed mutant p53 expression by immunohistochemical analysis of glioblastoma tissue samples. A significant association was found between MGMT methylation and p53 mutation status (p< .05). These results suggested that epigenetic inactivation of MGMT plays an important role in the survival of glioblastoma patients and this inactivated gene involved in p53 mutation.
Shamsara
Jamal
J
Biotechnology Research Center, Mashhad, Iran.
Sharif
Samaneh
S
Afsharnezhad
Sima
S
Lotfi
Marzieh
M
Raziee
Hamid Reza
HR
Ghaffarzadegan
Kamran
K
Moradi
Afshin
A
Rahighi
Saeed
S
Behravan
Javad
J
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001932:Brain Neoplasms / Q000737:chemistry / Q000201:enzymology / Q000235:genetics*; D002648:Child; D018899:CpG Islands; D019175:DNA Methylation; D015254:DNA Modification Methylases / Q000235:genetics*; D045643:DNA Repair Enzymes / Q000235:genetics*; D015972:Gene Expression Regulation, Neoplastic; D005909:Glioblastoma / Q000737:chemistry / Q000201:enzymology / Q000235:genetics*; D006801:Humans; D007150:Immunohistochemistry; D007492:Iran; D008875:Middle Aged; D009154:Mutation; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic; D016159:Tumor Suppressor Protein p53 / Q000032:analysis / Q000235:genetics*; D025521:Tumor Suppressor Proteins / Q000235:genetics*; D055815:Young Adult
C495901:TP53 protein, human; D016159:Tumor Suppressor Protein p53; D025521:Tumor Suppressor Proteins; D015254:DNA Modification Methylases; C515491:MGMT protein, human; D045643:DNA Repair Enzymes