Expression patterns of the Fam83h gene during murine tooth development.
OBJECTIVE
Recently a novel gene, FAM83H, was identified by a genetic linkage study in the hypocalcified form of the amelogenesis imperfecta family with an autosomal dominant hereditary pattern. Little is known about this novel gene, and so we investigated the expression pattern of Fam83h in murine tooth development using serial sectional in situ hybridisation.


METHODS
Using mandibles of ICR mouse at specific developmental stages, in situ hybridisation was performed by DIG-labeled RNA probe.


RESULTS
Faint expression was detected in limited cells at embryonic day 14 (E14) in the molar. At the bell stage, E16, Fam83h was localised in the outer and inner enamel epithelium, as well as dental papilla. Fam83h expression begins on E15 in the developing incisor. At E18, Fam83h was expressed in the inner enamel epithelium of the apical bud, ameloblasts and odontoblasts. The expression was stronger in the presecretory stages than the secretory stages.


CONCLUSIONS
Fam83h was detected in the ameloblasts from the presecretory to the secretory stage, and also the odontoblasts layer and surrounding alveolar bone.
Lee
Min-Jung
MJ
Department of Oral Biology, Research Center for Orofacial Hard Tissue Regeneration, Brain Korea 21 project, Oral Science Research Center, College of Dentistry, Yonsei Center of Biotechnology, Yonsei University, Seoul, Republic of Korea.
Lee
Sook-Kyung
SK
Lee
Kyung-Eun
KE
Kang
Hee-Yun
HY
Jung
Han-Sung
HS
Kim
Jung-Wook
JW
D000539:Alveolar Process / Q000196:embryology; D000565:Ameloblasts / Q000166:cytology; D000567:Amelogenesis Imperfecta / Q000235:genetics*; D000818:Animals; D003743:Dental Enamel / Q000196:embryology; D003771:Dental Papilla / Q000196:embryology; D004848:Epithelium / Q000196:embryology; D018507:Gene Expression Regulation, Developmental / Q000235:genetics; D017403:In Situ Hybridization; D007180:Incisor / Q000196:embryology; D008648:Mesoderm / Q000166:cytology; D051379:Mice; D008813:Mice, Inbred ICR; D008963:Molar / Q000196:embryology; D009804:Odontoblasts / Q000166:cytology; D009805:Odontogenesis / Q000235:genetics*; D011506:Proteins / Q000032:analysis / Q000235:genetics*; D014083:Tooth Germ / Q000196:embryology
D011506:Proteins