title: Novel strategy for measuring creatine kinase reaction rate in the in vivo heart.
abstract: In the heart, the creatine kinase (CK) system plays an important role in the cascade of ATP production, transportation, and utilization. The forward pseudo-first-order rate constant for the CK reaction can be measured noninvasively by the (31)P-magnetic resonance (MR) spectroscopy magnetization saturation transfer (MST) techniques. However, the measurement of MST in the in vivo heart is limited by the lengthy data acquisition time, especially for studies requiring spatial localization. This technical report presents a new method for measuring ATP production rate via CK that can reduce the MST data acquisition time by 82%. This method is validated using an in vivo pig model to evaluate the forward pseudo-first-order rate constant of myocardial CK reaction noninvasively.
authors: Cardiovascular Division, Department of Medicine, University of Minnesota Medical School, Minneapolis, Minnesota, USA.
authors: Qiang
authors: Q
authors: Xiong
authors: Qinglu
authors: Q
authors: Li
authors: Abdul
authors: A
authors: Mansoor
authors: Mohammad Nurulqadr
authors: MN
authors: Jameel
authors: Fei
authors: F
authors: Du
authors: Wei
authors: W
authors: Chen
authors: Jianyi
authors: J
authors: Zhang
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism*; D000818:Animals; D003402:Creatine Kinase / Q000378:metabolism*; D006333:Heart Failure / Q000175:diagnosis / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy / Q000379:methods* / Q000592:standards*; D008954:Models, Biological; D009206:Myocardium / Q000201:enzymology*; D010759:Phosphorus Isotopes; D051381:Rats; D015203:Reproducibility of Results; D034421:Sus scrofa
chemical_list: D010759:Phosphorus Isotopes; D000255:Adenosine Triphosphate; D003402:Creatine Kinase