title: Determination of cadmium and lead species and phytochelatins in pea (Pisum sativum) by HPLC-ICP-MS and HPLC-ESI-MSn.
abstract: An analytical approach based on hyphenated techniques was used for studying the speciation of cadmium and lead in Pisum sativum. Proper preservation conditions were employed to avoid the oxidation of -SH groups and corresponding decomposition of metal-binding complexes. SEC column was washed with 5 mM beta-mercaptoethanol and then samples were analysed using ICP-MS as a detector. Results showed that cadmium is the inhibitor of lead uptake. HPLC-ESI-MS(n) assays revealed fragmentation pathways of phytochelatins.
authors: Department of Trace Elements Analysis by Spectroscopy Method, Faculty of Chemistry, Adam Mickiewicz University, Grunwaldzka 6, 60-780 Poznań, Poland. danutaba@amu.edu.pl
authors: Danuta
authors: D
authors: Barałkiewicz
authors: Małgorzata
authors: M
authors: Kózka
authors: Aneta
authors: A
authors: Piechalak
authors: Barbara
authors: B
authors: Tomaszewska
authors: Paweł
authors: P
authors: Sobczak
mesh_terms: D002104:Cadmium / Q000032:analysis* / Q000737:chemistry; D002614:Chelating Agents / Q000032:analysis; D002851:Chromatography, High Pressure Liquid; D007854:Lead / Q000032:analysis* / Q000493:pharmacokinetics; D018532:Pisum sativum / Q000737:chemistry* / Q000378:metabolism; D054811:Phytochelatins / Q000378:metabolism*; D021241:Spectrometry, Mass, Electrospray Ionization; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D002614:Chelating Agents; D002104:Cadmium; D007854:Lead; D054811:Phytochelatins