Reconstruction of peri-implant bone defects using impacted bone allograft and BMP-2 gene-modified bone marrow stromal cells.
Impaction bone allografting represents an attractive procedure for bone defects reconstruction in joint replacement. And it was found that bone morphogenetic protein-2(BMP-2) gene therapy can enhance bone healing. The purpose of this study was to determine if combined adenovirus mediated human BMP-2(Adv-hBMP-2) gene-modified bone marrow stromal cells(BMSCs) with allograft enhanced the defects healing and improved the strength of implant fixation in 3-mm bone defect around a titanium alloy implant. Using the impaction grafting technique, the defects were reconstructed using freeze-dried allograft, freeze-dried allografts loaded with autogenous BMSCs, or freeze-dried allografts loaded with autogenous BMSCs modified with the human bone morphogenetic protein-2 (hBMP-2) gene. At 6 and 12 weeks, the Bone-implant Contact rate and strength of the interface in the group with BMP-2 gene medication were significantly higher than those of the non-cell or cell groups. BMP-2 gene medication also showed significant effects on allograft healing and replacement compared with those of two other groups, as evidenced by increased new bone formation and reduced graft remnants. The results suggest that BMP-2 gene medication can enhance allograft healing and osseointegration of the bone-implant interface.
Yan
Meng-ning
MN
Department of Orthopaedics, Ninth People's Hospital, Shanghai Jiaotong University School of Medicine, People's Republic of China.
Dai
Ke-rong
KR
Tang
Ting-ting
TT
Zhu
Zhen-an
ZA
Lou
Jue-ren
JR
D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology*; D055396:Bone Morphogenetic Protein 2 / Q000235:genetics* / Q000378:metabolism; D016025:Bone Transplantation; D001842:Bone and Bones / Q000000981:diagnostic imaging / Q000473:pathology* / Q000648:ultrastructure; D002448:Cell Adhesion; D049109:Cell Proliferation; D004285:Dogs; D005612:Freeze Drying; D006801:Humans; D020007:Implants, Experimental; D051379:Mice; D010012:Osteogenesis; D011859:Radiography; D019651:Plastic Surgery Procedures / Q000379:methods*; D033081:Shear Strength; D017154:Stromal Cells / Q000166:cytology* / Q000378:metabolism; D023822:Tissue Engineering; D014184:Transplantation, Homologous
C525718:BMP2 protein, human; D055396:Bone Morphogenetic Protein 2