title: Population structure and diversity of the pathogenic fungus Aspergillus fumigatus isolated from different sources and geographic origins.
abstract: Fifty-five clinical and environmental Aspergillus fumigatus isolates from Mexico, Argentina, France and Peru were analyzed to determine their genetic variability, reproductive system and level of differentiation using amplified fragment length polymorphism markers. The level of genetic variability was assessed by measuring the percentage of polymorphic loci, number of effective alleles, expected heterozygocity and by performing an association index test (I(A)). The degree of genetic differentiation and variation was determined using analysis of molecular variance at three levels. Using the paired genetic distances, a dendrogram was built to detect the genetic relationship among alleles. Finally, a network of haplotypes was constructed to determine the geographic relationship among them. The results indicate that the clinical isolates have greater genetic variability than the environmental isolates. The I(A) of the clinical and environmental isolates suggests a recombining population structure. The genetic differentiation among isolates and the dendrogram suggest that the groups of isolates are different. The network of haplotypes demonstrates that the majority of the isolates are grouped according to geographic origin.
authors: Departamento de Microbiología-Parasitología, Facultad de Medicina, Universidad Nacional Autónoma de México, México, DF, México.
authors: Esperanza
authors: E
authors: Duarte-Escalante
authors: Gerardo
authors: G
authors: Zúñiga
authors: Oscar Nava
authors: ON
authors: Ramírez
authors: Susana
authors: S
authors: Córdoba
authors: Nicolás
authors: N
authors: Refojo
authors: Roberto
authors: R
authors: Arenas
authors: Laurence
authors: L
authors: Delhaes
authors: María del Rocío
authors: Mdel R
authors: Reyes-Montes
mesh_terms: D054458:Amplified Fragment Length Polymorphism Analysis; D001118:Argentina; D001232:Aspergillus fumigatus / Q000235:genetics* / Q000302:isolation & purification / Q000502:physiology; D004777:Environment; D005602:France; D005787:Gene Frequency; D014644:Genetic Variation / Q000235:genetics*; D006239:Haplotypes; D008800:Mexico; D010568:Peru; D012098:Reproduction / Q000502:physiology