title: Molecular mechanisms of endothelial hyperpermeability: implications in inflammation.
abstract: Endothelial hyperpermeability is a significant problem in vascular inflammation associated with trauma, ischaemia-reperfusion injury, sepsis, adult respiratory distress syndrome, diabetes, thrombosis and cancer. An important mechanism underlying this process is increased paracellular leakage of plasma fluid and protein. Inflammatory stimuli such as histamine, thrombin, vascular endothelial growth factor and activated neutrophils can cause dissociation of cell-cell junctions between endothelial cells as well as cytoskeleton contraction, leading to a widened intercellular space that facilitates transendothelial flux. Such structural changes initiate with agonist-receptor binding, followed by activation of intracellular signalling molecules including calcium, protein kinase C, tyrosine kinases, myosin light chain kinase, and small Rho-GTPases; these kinases and GTPases then phosphorylate or alter the conformation of different subcellular components that control cell-cell adhesion, resulting in paracellular hypermeability. Targeting key signalling molecules that mediate endothelial-junction-cytoskeleton dissociation demonstrates a therapeutic potential to improve vascular barrier function during inflammatory injury.
authors: Division of Research, Department of Surgery, University of California at Davis School of Medicine, Sacramento, CA 95758, USA.
authors: Puneet
authors: P
authors: Kumar
authors: Qiang
authors: Q
authors: Shen
authors: Christopher D
authors: CD
authors: Pivetti
authors: Eugene S
authors: ES
authors: Lee
authors: Mack H
authors: MH
authors: Wu
authors: Sarah Y
authors: SY
authors: Yuan
mesh_terms: D000818:Animals; D002199:Capillary Permeability / Q000502:physiology*; D003599:Cytoskeleton / Q000378:metabolism; D004730:Endothelium, Vascular / Q000378:metabolism*; D006801:Humans; D007249:Inflammation / Q000378:metabolism* / Q000503:physiopathology*; D007365:Intercellular Junctions / Q000378:metabolism; D008954:Models, Biological