Scavenger receptor B2 is a cellular receptor for enterovirus 71.
Enterovirus 71 (EV71) belongs to human enterovirus species A of the genus Enterovirus within the family Picornaviridae. EV71, together with coxsackievirus A16 (CVA16), are most frequently associated with hand, foot and mouth disease (HFMD). Although HFMD is considered a mild exanthematous infection, infections involving EV71, but not CVA16, can progress to severe neurological disease, including fatal encephalitis, aseptic meningitis and acute flaccid paralysis. In recent years, epidemic and sporadic outbreaks of neurovirulent EV71 infections have been reported in Taiwan, Malaysia, Singapore, Japan and China. Here, we show that human scavenger receptor class B, member 2 (SCARB2, also known as lysosomal integral membrane protein II or CD36b like-2) is a receptor for EV71. EV71 binds soluble SCARB2 or cells expressing SCARB2, and the binding is inhibited by an antibody to SCARB2. Expression of human SCARB2 enables normally unsusceptible cell lines to support EV71 propagation and develop cytopathic effects. EV71 infection is hampered by the antibody to SCARB2 and soluble SCARB2. SCARB2 also supports the infection of the milder pathogen CVA16. The identification of SCARB2 as an EV71 and CVA16 receptor contributes to a better understanding of the pathogenicity of these viruses.
Yamayoshi
Seiya
S
Department of Microbiology and Immunology, Tokyo Metropolitan Institute for Neuroscience, Tokyo Metropolitan Organization for Medical Research, Tokyo, Japan.
Yamashita
Yasuko
Y
Li
Jifen
J
Hanagata
Nobutaka
N
Minowa
Takashi
T
Takemura
Taro
T
Koike
Satoshi
S
D000818:Animals; D029821:Enterovirus A, Human / Q000472:pathogenicity*; D020869:Gene Expression Profiling; D006801:Humans; D051907:Lysosomal Membrane Proteins / Q000032:analysis / Q000235:genetics / Q000502:physiology*; D051379:Mice; D051116:Receptors, Scavenger / Q000032:analysis / Q000235:genetics / Q000502:physiology*; D011991:Receptors, Virus / Q000032:analysis / Q000502:physiology*; D012208:Rhabdomyosarcoma / Q000821:virology
D051907:Lysosomal Membrane Proteins; D051116:Receptors, Scavenger; D011991:Receptors, Virus; C074299:SCARB2 protein, human