An oral Mycobacterium bovis BCG vaccine for wildlife produced in the absence of animal-derived reagents.
Cultures of Mycobacterium bovis BCG, comprising predominantly single-cell bacilli, were prepared in broth without animal-derived reagents. When formulated into a vegetable-derived lipid matrix, the vaccine was stable in vitro and was immunogenic in vivo upon feeding it to mice. This formulation could be useful for oral vaccination of wildlife against tuberculosis, where concern over transmissible prions may preclude the field use of vaccines containing animal products.
Cross
Martin L
ML
Centre for Innovation, University of Otago, P.O. Box 56, Dunedin, New Zealand. frank.cross@otago.ac.nz
Lambeth
Matthew R
MR
Aldwell
Frank E
FE
D000276:Adjuvants, Immunologic / Q000008:administration & dosage; D000284:Administration, Oral; D000818:Animals; D001500:BCG Vaccine / Q000008:administration & dosage / Q000276:immunology*; D003470:Culture Media / Q000737:chemistry*; D004355:Drug Stability; D005260:Female; D007371:Interferon-gamma / Q000378:metabolism; D007963:Leukocytes, Mononuclear / Q000276:immunology; D008055:Lipids / Q000008:administration & dosage; D051379:Mice; D008807:Mice, Inbred BALB C; D009163:Mycobacterium bovis / Q000254:growth & development*; D013154:Spleen / Q000276:immunology; D013678:Technology, Pharmaceutical / Q000379:methods*
D000276:Adjuvants, Immunologic; D001500:BCG Vaccine; D003470:Culture Media; D008055:Lipids; D007371:Interferon-gamma