Production of yellow fever virus in microcarrier-based Vero cell cultures.
In this work, the propagation of the 17DD yellow fever virus in Vero cells grown on Cytodex-1 microcarriers was evaluated. After verifying that upon infection the virus adsorption step could be performed under continuous agitation, experiments were carried out in spinners and sparged lab-scale stirred-tank bioreactor to evaluate the use of a commercial serum-free medium (VP-SFM) and to investigate the effects of multiplicity of infection (MOI) and time of infection (TOI) on virus production. Virus titers as high as 8.4 x 10(8)pfu/mL were obtained upon infection with MOI of 0.02 and TOI of 3 days, using the serum-free medium in the sparged bioreactor.
Souza
Marta Cristina O
MC
Oswaldo Cruz Foundation (FIOCRUZ), Bio-Manguinhos, Viral Vaccine Program, Avenida Brasil 4365, 21045-900 Rio de Janeiro/RJ, Brazil.
Freire
Marcos S
MS
Schulze
Erica A
EA
Gaspar
Luciane P
LP
Castilho
Leda R
LR
D000818:Animals; D019149:Bioreactors; D018929:Cell Culture Techniques; D002522:Chlorocebus aethiops; D016895:Culture Media, Serum-Free; D014709:Vero Cells; D014776:Virus Cultivation / Q000379:methods*; D015005:Yellow fever virus / Q000254:growth & development*
D016895:Culture Media, Serum-Free