Downregulation of pro-inflammatory cytokines by lupeol measured using cytometric bead array immunoassay.
The objective of the study was to investigate the activity of Lupeol (LUP) on proinflammatory and anti-inflammatory cytokines in the pleural exudate from male swiss albino mice. We applied Cytometric bead array technology for simultaneously measurement of these cytokines in pleurisy induced mice treated with lupeol in graded oral doses. Cytometric bead array uses the sensitivity of amplified fluorescence detection by flowcytometer to measure soluble analytes in a particle based immune assay. This assay can accurately quantitate 5 cytokines in a 50 microlitre sample volume. Oral administration of LUP at doses of 25, 50, 100 and 200 mg/kg p.o. produced dose related inhibition of IL-2, IFN-gamma and TNF-alpha in the pleural exudate with the most significant effect at 100 mg/kg oral dose. LUP had a non significant inhibitory effect on the levels of IL-4 and IL-5.
Ahmad
Sheikh Fayaz
SF
Cell Biology Laboratory, Department of Pharmacology, Indian Institute of Integrative Medicine, CSIR, Canal Road, Jammu Tawi 180001, Jammu and Kashmir State, India.
Pandey
Anjali
A
Kour
Kiranjeet
K
Bani
Sarang
S
D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D015536:Down-Regulation; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D007371:Interferon-gamma / Q000378:metabolism*; D007376:Interleukin-2 / Q000378:metabolism*; D015847:Interleukin-4 / Q000378:metabolism; D015848:Interleukin-5 / Q000378:metabolism; D008297:Male; D051379:Mice; D053978:Pentacyclic Triterpenes / Q000494:pharmacology*; D010936:Plant Extracts / Q000494:pharmacology; D010996:Pleural Effusion / Q000276:immunology; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*; D000094042:Lupanes
D000893:Anti-Inflammatory Agents; D007371:Interferon-gamma; D007376:Interleukin-2; D015847:Interleukin-4; D015848:Interleukin-5; D053978:Pentacyclic Triterpenes; D010936:Plant Extracts; D014409:Tumor Necrosis Factor-alpha; C010480:lupeol; D000094042:Lupanes