Assessment of new 2'-O-acetalester protecting groups for regular RNA synthesis and original 2'-modified proRNA.
New base-labile acyloxymethyl groups were evaluated to protect 2'-OH functions of ribonucleotides for regular RNA synthesis in order to shorten the deprotection procedure upon ammonia. These same acetalester groups were assessed in 2'-modified proRNA as biolabile 2'-protections removable by cell enzymes to generate parent RNA. Demasking of 2'-modified pro-uridylates was studied in cell extracts.
Martin
Anthony R
AR
IBMM, UMR 5247 CNRS-UM1-UM2, cc1704, Université Montpellier 2, Place Eugène Bataillon, 34095 Montpellier Cedex 05, France.
Lavergne
Thomas
T
Vasseur
Jean-Jacques
JJ
Debart
Françoise
F
D000641:Ammonia / Q000737:chemistry; D002460:Cell Line; D002625:Chemistry, Organic / Q000379:methods; D002626:Chemistry, Pharmaceutical / Q000379:methods; D015195:Drug Design; D004952:Esters / Q000737:chemistry; D006801:Humans; D006868:Hydrolysis; D008956:Models, Chemical; D009843:Oligoribonucleotides / Q000737:chemistry; D012313:RNA / Q000737:chemistry*; D034622:RNA Interference; D012265:Ribonucleotides / Q000737:chemistry*; D013696:Temperature
D004952:Esters; D009843:Oligoribonucleotides; D012265:Ribonucleotides; D012313:RNA; D000641:Ammonia