title: Degradome expression profiling in human articular cartilage.
abstract: BACKGROUND
  The molecular mechanisms underlying cartilage destruction in osteoarthritis are poorly understood. Proteolysis is a key feature in the turnover and degradation of cartilage extracellular matrix where the focus of research has been on the metzincin family of metalloproteinases. However, there is strong evidence to indicate important roles for other catalytic classes of proteases, with both extracellular and intracellular activities. The aim of this study was to profile the expression of the majority of protease genes in all catalytic classes in normal human cartilage and that from patients with osteoarthritis (OA) using a quantitative method.
  
  
  METHODS
  Human cartilage was obtained from femoral heads at joint replacement for either osteoarthritis or following fracture to the neck of femur (NOF). Total RNA was purified, and expression of genes assayed using Taqman low-density array quantitative RT-PCR.
  
  
  RESULTS
  A total of 538 protease genes were profiled, of which 431 were expressed in cartilage. A total of 179 genes were differentially expressed in OA versus NOF cartilage: eight aspartic proteases, 44 cysteine proteases, 76 metalloproteases, 46 serine proteases and five threonine proteases. Wilcoxon ranking as well as the LogitBoost-NR machine learning approach were used to assign significance to each gene, with the most highly ranked genes broadly similar using each method.
  
  
  CONCLUSIONS
  This study is the most complete quantitative analysis of protease gene expression in cartilage to date. The data help give direction to future research on the specific function(s) of individual proteases or protease families in cartilage and may help to refine anti-proteolytic strategies in OA.
authors: School of Biological Sciences, University of East Anglia, Norwich NR4 7TJ, UK. t.swingler@uea.ac.uk
authors: Tracey E
authors: TE
authors: Swingler
authors: Jasmine G
authors: JG
authors: Waters
authors: Rosemary K
authors: RK
authors: Davidson
authors: Caroline J
authors: CJ
authors: Pennington
authors: Xose S
authors: XS
authors: Puente
authors: Clare
authors: C
authors: Darrah
authors: Adele
authors: A
authors: Cooper
authors: Simon T
authors: ST
authors: Donell
authors: Geoffrey R
authors: GR
authors: Guile
authors: Wenjia
authors: W
authors: Wang
authors: Ian M
authors: IM
authors: Clark
mesh_terms: D051722:ADAM Proteins / Q000096:biosynthesis / Q000235:genetics; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002358:Cartilage, Articular / Q000201:enzymology / Q000473:pathology* / Q000502:physiology; D004792:Enzyme Precursors / Q000096:biosynthesis / Q000235:genetics; D005260:Female; D005270:Femur Head / Q000201:enzymology / Q000378:metabolism / Q000473:pathology; D020869:Gene Expression Profiling / Q000379:methods*; D006801:Humans; D008297:Male; D020782:Matrix Metalloproteinases / Q000096:biosynthesis / Q000235:genetics; D008875:Middle Aged; D010003:Osteoarthritis / Q000201:enzymology / Q000378:metabolism / Q000473:pathology; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D004792:Enzyme Precursors; D051722:ADAM Proteins; D020782:Matrix Metalloproteinases