Combined microwave energy and fixative agent for cataract induction in pig eyes.
We describe a technique used in enucleated pig eyes to create a uniformly hardened lens nucleus and an anterior capsule similar to the cataractous human lens. After treatment with microwave heat and a fixative agent, the pig eyes have clear corneas, anterior capsules with less tension and elasticity, and harder lens nuclei, with a consistency similar to that of a nuclear sclerosis grade I to V in the Emery-Little classification. The eyes can be used by residents in training to practice various phacoemulsification techniques, including continuous curvilinear capsulorhexis, hydrodelineation, sculpting, divide and conquer, phaco chop, and nondividing phacoemulsification.
Shentu
Xingchao
X
Eye Center, Affiliated Second Hospital, College of Medicine, Zhejiang University, Hangzhou, China.
Tang
Xiajing
X
Ye
Panpan
P
Yao
Ke
K
D000818:Animals; D002386:Cataract / Q000209:etiology* / Q000473:pathology; D003315:Cornea / Q000502:physiology; D004195:Disease Models, Animal; D004338:Drug Combinations; D015353:Eye Enucleation; D005404:Fixatives / Q000633:toxicity*; D005557:Formaldehyde / Q000633:toxicity; D007908:Lens, Crystalline / Q000187:drug effects* / Q000528:radiation effects*; D000432:Methanol / Q000633:toxicity; D008872:Microwaves; D018918:Phacoemulsification / Q000193:education*; D013552:Swine
D004338:Drug Combinations; D005404:Fixatives; D005557:Formaldehyde; D000432:Methanol