Use of quantum dots to detect human papillomavirus in oral squamous cell carcinoma.
OBJECTIVE
To examine the association of oral squamous cell carcinoma with human papillomavirus (HPV) using quantum dots (QD) in situ hybridization (ISH).


METHODS
Expression of HPV16/18 was analyzed in a representative collection of 21 oral squamous cell carcinomas by tissue microarrays. The presence of HPV16/18 high risk was detected by applying QDISH which is compared with conventional ISH.


RESULTS
Seven cases out of 21 (33.3%) were positive for QDISH while 1 out of 21 (4.8%) was positive for ISH, although all of HPV DNA were localized in the nuclei in the spinous and basal cell layer of the epithelium. The difference between these two methods was significant (P < 0.05).


CONCLUSIONS
These results demonstrate that the QD might be an efficient method for determination of HPV infection and HPV-associated oral squamous cell carcinoma.
Xue
J
J
Department of Pathology and Pathophysiology, School of Basic Medical Science, Wuhan University, Wuhan 430079, China.
Chen
H
H
Fan
M
M
Zhu
F
F
Diao
L
L
Chen
X
X
Fan
L
L
Li
P
P
Xia
D
D
D002294:Carcinoma, Squamous Cell / Q000473:pathology / Q000821:virology*; D005260:Female; D006801:Humans; D017403:In Situ Hybridization / Q000379:methods*; D008297:Male; D046228:Microarray Analysis / Q000379:methods; D008875:Middle Aged; D009062:Mouth Neoplasms / Q000473:pathology / Q000821:virology*; D027383:Papillomaviridae / Q000302:isolation & purification*; D030361:Papillomavirus Infections / Q000473:pathology / Q000821:virology*; D045663:Quantum Dots; D012680:Sensitivity and Specificity