title: Cell nanomechanics and focal adhesions are regulated by retinol and conjugated linoleic acid in a dose-dependent manner.
abstract: Retinol and conjugated linoleic acid (CLA) have previously been shown to have an important role in gene expression and various cellular processes, including differentiation, proliferation and cell death. In this study we have investigated the effect of retinol and CLA, both individually and in combination, on the intracellular cytoskeleton, focal adhesions (FAs) and the nanomechanical properties of 3T3 fibroblasts. We observed a dose-dependent decrease in the formation of FAs following treatment with either compound, which was directly correlated to an increase in cell height (>30%) and a decrease in the measured Young's modulus (approximately 28%). Furthermore, treatments with both compounds demonstrated an increased effect and led to a reduction of >70% in the average number of FAs per cell and a decrease of >50% in average cell stiffness. These data reveal that retinol and CLA disrupt FA formation, leading to an increase in cell height and a significant decrease in stiffness. These results may broaden our understanding of the interplay between cell nanomechanics and cellular contact with the external microenvironment, and help to shed light on the important role of retinoids and CLA in health and disease.
authors: The London Centre for Nanotechnology and Centre for NanoMedicine, University College London, London, UK.
authors: Yaron R
authors: YR
authors: Silberberg
authors: Gleb E
authors: GE
authors: Yakubov
authors: Michael A
authors: MA
authors: Horton
authors: Andrew E
authors: AE
authors: Pelling
mesh_terms: D016475:3T3 Cells; D000818:Animals; D001696:Biomechanical Phenomena / Q000187:drug effects*; D048430:Cell Shape / Q000187:drug effects; D003599:Cytoskeleton / Q000187:drug effects / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D022001:Focal Adhesions / Q000378:metabolism*; D044243:Linoleic Acids, Conjugated / Q000494:pharmacology*; D051379:Mice; D018625:Microscopy, Atomic Force; D018613:Microscopy, Confocal; D014801:Vitamin A / Q000494:pharmacology*; D014815:Vitamins / Q000494:pharmacology*
chemical_list: D044243:Linoleic Acids, Conjugated; D014815:Vitamins; D014801:Vitamin A