title: Irradiation enhances the efficiency of testicular germ cell transplantation in sheep.
abstract: Testis germ cell transplantation in livestock has the potential for production of transgenic genotypes and for use as an alternative to artificial insemination in animal breeding systems. In a pilot experiment, we investigated a workable protocol for testis germ cell transplantation in sheep, including donor cell isolation, rete testis injection, and microsatellite detection of donor spermatozoa in recipient semen. In a second experiment, the effect of depletion of endogenous stem cells with a single irradiation dose of 9 Gy (n = 5) or 15 Gy (n = 5) on the outcome of germ cell transplantation was investigated. Irradiation of recipient testes with a single dose of 15 Gy, followed by transplantation 6 wk after depletion, may be most advantageous because it resulted in all recipients (five of five) producing donor-derived spermatozoa, while the 9-Gy and control groups had limited success rates (two of five and one of three, respectively). Using microsatellite markers to detect the presence of donor DNA, 10 rams were identified that produced spermatozoa of donor origin. The proportion of donor DNA was between 1% and 30% of total ejaculate DNA. When three of these positive rams were used in breeding experiments, four donor-derived offspring (four of 50 [8% of progeny])resulted from a recipient in Merino to Merino transplantation. Six lambs (six of 41 [15% of progeny]) were sired by donor-derived Border Leicester sperm produced in a Merino recipient ram; however, no donor-derived offspring were detected among 34 progeny from a second Border Leicester to Merino combination. These results confirm that preparation of recipient animals with a correct dose of irradiation not only enhances the success rate of the transplantation procedure but also increases the proportion of donor spermatozoa in recipient semen. This study represents the first report of the production of live progeny following testis germ cell transplantation using irradiated recipients in a livestock species.
authors: CSIRO Livestock Industries, FD McMaster Laboratory, Armidale, New South Wales, Australia. muren.herrid@csiro.au
authors: Muren
authors: M
authors: Herrid
authors: Jeanette
authors: J
authors: Olejnik
authors: Michael
authors: M
authors: Jackson
authors: Natalka
authors: N
authors: Suchowerska
authors: Sally
authors: S
authors: Stockwell
authors: Rhonda
authors: R
authors: Davey
authors: Keryn
authors: K
authors: Hutton
authors: Shelly
authors: S
authors: Hope
authors: Jonathan R
authors: JR
authors: Hill
mesh_terms: D000818:Animals; D017690:Cell Transplantation / Q000379:methods; D004973:Estrus Synchronization; D005260:Female; D005838:Genotype; D007150:Immunohistochemistry; D007315:Insemination, Artificial; D008297:Male; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012661:Semen; D012756:Sheep; D013094:Spermatozoa / Q000528:radiation effects* / Q000637:transplantation*; D013737:Testis / Q000166:cytology / Q000528:radiation effects*