Therapeutic monoclonal antibody treatment targeting respiratory syncytial virus (RSV) G protein mediates viral clearance and reduces the pathogenesis of RSV infection in BALB/c mice.
Because the G protein of respiratory syncytial virus (RSV) has a CX3C chemokine motif that has been associated with the ability of RSV G protein to modulate the virus-induced host immune response, we examined whether therapeutic treatment with an anti-RSV G monoclonal antibody (mAb), 131-2G, that blocks the CX3C-associated activity of RSV G protein might decrease the pulmonary inflammation associated with infection in BALB/c mice. The results show that treatment with mAb 131-2G on day 3 after RSV infection reduces both inflammation and RSV titer in the lungs. Later administration of anti-RSV G mAb (day 5 after RSV infection) effectively reduced the viral titer but had a minimal effect on pulmonary inflammation. This study suggests that an anti-RSV G mAb might be an effective antiviral, either alone or in combination with anti-RSV F protein neutralizing antibodies, for decreasing the virus-induced host response to infection and improve treatment outcome.
Haynes
Lia M
LM
National Centers for Immunization and Respiratory Diseases, Div of Viral Diseases, Respiratory and Gastroenteritis Viruses Branch, 1600 Clifton Rd NE, Mailstop G-18, Atlanta, GA 30333, USA. loh5@cdc.gov
Caidi
Hayat
H
Radu
Gertrud U
GU
Miao
Congrong
C
Harcourt
Jennifer L
JL
Tripp
Ralph A
RA
Anderson
Larry J
LJ
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology* / Q000627:therapeutic use*; D000914:Antibodies, Viral; D004306:Dose-Response Relationship, Immunologic; D005260:Female; D008171:Lung Diseases / Q000276:immunology / Q000473:pathology / Q000821:virology; D051379:Mice; D008807:Mice, Inbred BALB C; D018357:Respiratory Syncytial Virus Infections / Q000276:immunology*; D012136:Respiratory Syncytial Viruses / Q000276:immunology*; D013047:Specific Pathogen-Free Organisms; D014760:Viral Fusion Proteins / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; C502275:G glycoprotein, Respiratory syncytial virus; D014760:Viral Fusion Proteins