Metabonomic characterization of early atherosclerosis in hamsters with induced cholesterol.
Atherosclerosis is a complicated and multifactorial disease, induced not only by genotype, but also, even more importantly, by environmental factors. Study on the metabolic perturbation of endogenous compounds may offer deeper insight into development of atherosclerosis. Gas chromatography/mass spectrometry (GC/MS)-based metabonomics was used to profile a metabolic fingerprint of serum obtained from hamsters with induced cholesterol. The deconvoluted GC/MS data were processed by multivariate statistical analysis tools, such as principal component analysis (PCA) and partial least squares projection to latent structure and discriminant analysis (PLS-DA). For the first time we showed a time-dependent development of the model animal from normal to hypercholesterolaemia, and further to early atherosclerosis. Twenty-one compounds were identified as markers involved in the development to atherosclerosis. Identification of the compounds suggests that amino acid metabolism and fatty acid oxidation are significantly perturbed following cholesterol overloading. The data provide novel information to approach the pathophysiological processes of the hypercholesterolaemia and atherosclerosis disease continuum.
Zha
Weibin
W
Key Laboratory of Drug Metabolism and Pharmacokinetics, China Pharmaceutical University, Nanjing, China.
A
Jiye
J
Wang
Guangji
G
Yan
Bei
B
Gu
Shenghua
S
Zhu
Xuanxuan
X
Hao
Haiping
H
Huang
Qing
Q
Sun
Jianguo
J
Zhang
Ying
Y
Cao
Bei
B
Ren
Hongcan
H
D000596:Amino Acids / Q000378:metabolism; D000818:Animals; D050197:Atherosclerosis / Q000209:etiology / Q000378:metabolism*; D002784:Cholesterol / Q000378:metabolism*; D006224:Cricetinae; D005227:Fatty Acids / Q000378:metabolism; D008401:Gas Chromatography-Mass Spectrometry; D006937:Hypercholesterolemia / Q000378:metabolism; D055432:Metabolomics; D010084:Oxidation-Reduction
D000596:Amino Acids; D005227:Fatty Acids; D002784:Cholesterol