Overexpression of the HMG-CoA reductase gene leads to enhanced artemisinin biosynthesis in transgenic Artemisia annua plants.
An effective and affordable treatment against malaria is still a challenge for medicine. Most contemporary drugs either are too expensive to produce or are not effective against resistant strains of the malaria parasite Plasmodium falciparum. The plant Artemisia annua L. is the source of artemisinin, an effective drug against malaria for which no resistant strains of the bacterium have been reported. However, the artemisinin content of A. annua is very low, which makes its production expensive. Here we report the use of transgenic technology to increase the artemisinin content of A. annua. We report the production of transgenic plants of A. annua into which we transferred 3-hydroxy-3-methylglutaryl CoA reductase (HMGR) gene from Catharanthus roseus (L.) G. Don using Agrobacterium-mediated gene transfer technology. Transgene integration and copy number were assessed by PCR and Southern hybridization, which confirmed the stable integration of multiple copies of the transgene in 7 different transgenic lines of A. annua. The leaf tissue of three of the A. annua transgenic lines possessed significantly higher HMGR activity compared with wild-type controls, and this activity was associated exclusively with microsomal membranes. The artemisinin content of the shoots of one of the transgenic lines depicted an increase of 22.5 % artemisinin content compared with wild-type control A. annua plants.
Aquil
Samina
S
Centre for Transgenic Plant Development, Department of Biotechnology, Jamia Hamdard, New Delhi, India.
Husaini
Amjad Masood
AM
Abdin
Malik Zainul
MZ
Rather
Gulam Muhammad
GM
D000962:Antimalarials / Q000378:metabolism; D031172:Artemisia annua / Q000235:genetics* / Q000378:metabolism; D037621:Artemisinins / Q000378:metabolism*; D015139:Blotting, Southern; D030008:Catharanthus / Q000235:genetics; D015870:Gene Expression; D017343:Genes, Plant / Q000502:physiology*; D006903:Hydroxymethylglutaryl CoA Reductases / Q000235:genetics* / Q000378:metabolism; D007425:Intracellular Membranes; D008861:Microsomes; D010936:Plant Extracts / Q000235:genetics / Q000378:metabolism*; D018515:Plant Leaves; D030821:Plants, Genetically Modified; D016133:Polymerase Chain Reaction; D012231:Rhizobium
D000962:Antimalarials; D037621:Artemisinins; D010936:Plant Extracts; C031327:artemisinin; D006903:Hydroxymethylglutaryl CoA Reductases