The adaptor protein LAD/TSAd mediates laminin-dependent T cell migration via association with the 67 kDa laminin binding protein.
The adaptor protein, LAD/TSAd, plays essential roles in T cell activation. To further understand the functions of this protein, we performed yeast two-hybrid screening using TSAd as bait and identified 67 kDa laminin binding protein (LBP) as the interacting partner. Subsequently, TSAd-LBP interaction was confirmed in D1.1 T cell line. Upon costimulation by T cell receptor (TCR) plus laminin crosslinking or TCR plus integrin alpha6 crosslinking, LBP was coimmunoprecipitated with TSAd. Moreover, TCR plus laminin costimulation-dependent T cell migration was enhanced in D1.1 T cells overexpressing TSAd but was disrupted in D1.1 cells overexpressing dominant negative form of TSAd or TSAd shRNA. These data show that, upon TCR plus integrin costimulation, TSAd associates with LBP and mediates T lymphocyte migration.
Park
Eunkyung
E
Department of Life Science, Ewha Womans' University, Seoul 120-750, Korea.
Choi
Youngbong
Y
Ahn
Eunseon
E
Park
Inyoung
I
Yun
Yungdae
Y
D048868:Adaptor Proteins, Signal Transducing / Q000235:genetics / Q000378:metabolism*; D000599:Amino Acids, Diamino / Q000378:metabolism; D002352:Carrier Proteins / Q000378:metabolism*; D002465:Cell Movement; D006801:Humans; D039503:Integrin alpha6 / Q000378:metabolism; D019169:Jurkat Cells; D009154:Mutation; D011485:Protein Binding; D034741:RNA, Small Interfering / Q000235:genetics; D020239:Receptor Cross-Talk; D011948:Receptors, Antigen, T-Cell / Q000378:metabolism; D013601:T-Lymphocytes / Q000378:metabolism*; D019076:Transgenes; D020798:Two-Hybrid System Techniques
D048868:Adaptor Proteins, Signal Transducing; D000599:Amino Acids, Diamino; D002352:Carrier Proteins; D039503:Integrin alpha6; D034741:RNA, Small Interfering; D011948:Receptors, Antigen, T-Cell; C405691:SH2D2A protein, human; C045397:laminine