Pontibacillus halophilus sp. nov., a moderately halophilic bacterium isolated from a sea urchin.
A Gram-positive-staining, moderately halophilic, strictly aerobic, catalase- and oxidase-positive, rod-shaped bacterium, designated strain JSM 076056(T), was isolated from a sea urchin collected from the South China Sea. Cells were motile by means of peritrichous flagella and formed ellipsoidal endospores lying in subterminal swollen sporangia. Strain JSM 076056(T) was able to grow at salinities of 2-25 % (w/v) total salts and at pH 6.0-10.0 and 15-40 degrees C; optimum growth was observed with 5-10 % (w/v) total salts and at pH 7.0-8.0 and 25-30 degrees C. It was incapable of growing with NaCl as the sole salt. meso-Diaminopimelic acid was present in the cell-wall peptidoglycan. The major cellular fatty acids were iso-C(15 : 0), iso-C(16 : 0), iso-C(14 : 0,) anteiso-C(15 : 0) and C(16 : 1)omega7c alcohol. The predominant respiratory quinone was MK-7 and the genomic DNA G+C content was 45.5 mol%. A phylogenetic analysis based on 16S rRNA gene sequences indicated that strain JSM 076056(T) belonged to the family Bacillaceae and was related most closely to the type strains of the two recognized species of the genus Pontibacillus, namely Pontibacillus chungwhensis BH030062(T) (96.4 % sequence similarity) and Pontibacillus marinus BH030004(T) (96.2 %); these three strains formed a robust cluster in the phylogenetic tree. In combination, the phylogenetic, phenotypic and chemotaxonomic data indicate that strain JSM 076056(T) represents a novel species of the genus Pontibacillus, for which the name Pontibacillus halophilus sp. nov. is proposed. The type strain is JSM 076056(T) (=CCTCC AA 207029(T) =DSM 19796(T) =KCTC 13190(T)).
Chen
Yi-Guang
YG
Key Laboratory for Conservation and Utilization of Plant Resources of Hunan Province, College of Biology and Environmental Sciences, Jishou University, Jishou, Hunan 416000, PR China. mchenjsu@yahoo.com.cn
Zhang
Yu-Qin
YQ
Xiao
Huai-Dong
HD
Liu
Zhu-Xiang
ZX
Yi
Lang-Bo
LB
Shi
Jin-Xiao
JX
Zhi
Xiao-Yang
XY
Cui
Xiao-Long
XL
Li
Wen-Jun
WJ
D000818:Animals; D001406:Bacillaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology; D015373:Bacterial Typing Techniques; D001482:Base Composition; D002681:China; D004269:DNA, Bacterial / Q000032:analysis; D005227:Fatty Acids / Q000032:analysis; D020459:Genes, rRNA; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010641:Phenotype; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012617:Sea Urchins / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D012965:Sodium Chloride; D013045:Species Specificity
D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D012965:Sodium Chloride