title: Improved phase sensitivity in spectral domain phase microscopy using line-field illumination and self phase-referencing.
abstract: We report a quantitative phase microscope based on spectral domain optical coherence tomography and line-field illumination. The line illumination allows self phase-referencing method to reject common-mode phase noise. The quantitative phase microscope also features a separate reference arm, permitting the use of high numerical aperture (NA > 1) microscope objectives for high resolution phase measurement at multiple points along the line of illumination. We demonstrate that the path-length sensitivity of the instrument can be as good as 41 pm/square root of Hz, which makes it suitable for nanometer scale study of cell motility. We present the detection of natural motions of cell surface and two-dimensional surface profiling of a HeLa cell.
authors: G. R. Harrison Spectroscopy Laboratory, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA.
authors: Zahid
authors: Z
authors: Yaqoob
authors: Wonshik
authors: W
authors: Choi
authors: Seungeun
authors: S
authors: Oh
authors: Niyom
authors: N
authors: Lue
authors: Yongkeun
authors: Y
authors: Park
authors: Christopher
authors: C
authors: Fang-Yen
authors: Ramachandra R
authors: RR
authors: Dasari
authors: Kamran
authors: K
authors: Badizadegan
authors: Michael S
authors: MS
authors: Feld
mesh_terms: D002462:Cell Membrane / Q000378:metabolism; D004867:Equipment Design; D006367:HeLa Cells; D006801:Humans; D007834:Lasers; D008853:Microscopy / Q000295:instrumentation / Q000379:methods*; D009474:Neurons / Q000473:pathology; D055095:Optics and Photonics; D013053:Spectrophotometry / Q000379:methods; D013499:Surface Properties; D041623:Tomography, Optical Coherence / Q000379:methods*