Evaluation of Blatta orientalis (Q) nasal gel formulation in milk aspiration induced eosinophilia.
BACKGROUND
The purpose of the present study was to develop intranasal delivery systems of the homeopathic anti-asthmatic remedy Blatta orientalis mother tincture (Q) using thermoreversible polymer Pluronic F127 (PF127) and mucoadhesive polymer Carbopol 934P (C934P).


METHODS
Formulations were modulated so as to have a gelation temperature below 34 degrees C to ensure gelation at physiological temperature after intranasal administration. Its gelation temperature, mucoadhesive strength, viscosity and gel strength were studied. B. orientalis (Q) nasal gel was tested with recurrent milk aspiration to determine whether it produces changes in eosinophilia in a murine model of asthma.


RESULTS
The gelation temperatures of the formulations and mucoadhesive strength, determined using sheep nasal mucosal membrane, increased by the addition of increasing concentrations of Carbopol. The results of milk aspiration induced eosinophilia, B. orientalis (Q) nasal gel significantly (P < 0.001), decreased eosinophil cell count as compared with toxicant by using in absolute eosinophilia count method. Finally, histopathological examination did not detect any damage during in vivo studies.


CONCLUSIONS
The PF127 gel formulation of B. orientalis (Q) with in situ gelling and mucoadhesive properties with increased permeation rate is promising for prolonging nasal residence time and thereby nasal absorption.
Nimgulkar
Chetan Chandrakant
CC
Dept. of Pharmacology, R.C. Patel Institute of Pharmaceutical Education and Research, Shirpur, Dhule Maharashtra State, India. chetanmhrpharma@gmail.com
Patil
Savita Dattatray
SD
Chauk
Dheeraj S
DS
D000179:Acrylates / Q000737:chemistry; D000268:Adhesiveness; D000281:Administration, Intranasal; D000818:Animals; D001249:Asthma / Q000188:drug therapy / Q000503:physiopathology; D003058:Cockroaches / Q000737:chemistry*; D004195:Disease Models, Animal; D016503:Drug Delivery Systems; D005079:Excipients / Q000737:chemistry*; D005260:Female; D005782:Gels; D006168:Guinea Pigs; D006705:Homeopathy; D008297:Male; D051379:Mice; D008892:Milk; D009297:Nasal Mucosa / Q000378:metabolism; D010539:Permeability; D020442:Poloxamer / Q000737:chemistry; D011657:Pulmonary Eosinophilia / Q000188:drug therapy* / Q000209:etiology; D051381:Rats; D017208:Rats, Wistar; D053120:Respiratory Aspiration / Q000150:complications*; D012756:Sheep; D013696:Temperature; D014783:Viscosity
D000179:Acrylates; D005079:Excipients; D005782:Gels; D020442:Poloxamer; C453622:carbopol 934P