Dual-thermoresponsive phase behavior of blood compatible zwitterionic copolymers containing nonionic poly(N-isopropyl acrylamide).
Thermoresponsive statistical copolymers of zwitterionic sulfobetaine methacrylate (SBMA) and nonionic N-isopropylacrylamide (NIPAAm) were prepared with an average molecular weight of about 6.0 kDa via homogeneous free radical copolymerization. The aqueous solution properties of poly(SBMA-co-NIPAAm) were measured using a UV--visible spectrophotometer. The copolymers exhibited controllable lower and upper critical solution temperatures in aqueous solution and showed stimuli-responsive phase transition in the presence of salts. Regulated zwitterionic and nonionic molar mass ratios led to poly(SBMA-co-NIPAAm) copolymers having double-critical solution temperatures, where the water-insoluble polymer microdomains are generated by the zwitterionic copolymer region of polySBMA or nonionic copolymer region of polyNIPAAm depending on temperature. A high content of the nonionic polyNIPAAm in poly(SBMA-co-NIPAAm) exhibits nonionic aggregation at high temperatures due to the desolvation of polyNIPAAm, whereas relatively low content of polyNIPAAm in poly(SBMA-co-NIPAAm) exhibits zwitterionic aggregation at low temperatures due to the desolvation of polySBMA. Plasma protein adsorption on the surface coated with poly(SBMA-co-NIPAAm) was measured with a surface plasmon resonance (SPR) sensor. The copolymers containing polySBMA above 29 mol % showed extremely low protein adsorption and high anticoagulant activity in human blood plasma. The tunable and switchable thermoresponsive phase behavior of poly(SBMA-co-NIPAAm), as well as its high plasma protein adsorption resistance and anticoagulant activity, suggests a potential for blood-contacting applications.
Chang
Yung
Y
Department of Chemical Engineering, R&D Center for Membrane Technology, Chung Yuan Christian University, Jhong-Li, Taoyuan 320, Taiwan.
Chen
Wen-Yih
WY
Yandi
Wetra
W
Shih
Yu-Ju
YJ
Chu
Wan-Ling
WL
Liu
Ying-Ling
YL
Chu
Chih-Wei
CW
Ruaan
Ruoh-Chyu
RC
Higuchi
Akon
A
D000178:Acrylamides / Q000737:chemistry*; D000925:Anticoagulants / Q000737:chemistry*; D001672:Biocompatible Materials / Q000737:chemistry*; D001798:Blood Proteins / Q000737:chemistry*; D006046:Gold / Q000737:chemistry; D006801:Humans; D044367:Phase Transition; D011108:Polymers / Q000737:chemistry*; D020349:Surface Plasmon Resonance; D013499:Surface Properties; D013696:Temperature
D000178:Acrylamides; D000925:Anticoagulants; D001672:Biocompatible Materials; D001798:Blood Proteins; D011108:Polymers; D006046:Gold; C067295:N-isopropylacrylamide