Soluble angiopoietin receptor Tie-2 in patients with acute myocardial infarction and its detection by optical protein-chip.
The Tie-2 receptor has been shown to play a role in angiogenesis in atherosclerosis. The conventional method assaying the level of soluble Tie-2 (sTie-2) was ELISA. However, this method has some disadvantages. The aims of this research are to establish a more simple detection method, the optical protein-chip based on imaging ellipsomtry (OPC-IE) applying to Tie-2 assay. The sTie-2 biosensor surface on silicon wafer was prepared first, and then serum levels of sTie-2 in 38 patients with AMI were measured on admission (day 1), day 2, day 3 and day 7 after onset of chest pain and 41 healthy controls by ELISA and OPC-IE in parallel. Median level of sTie-2 increased significantly in the AMI patients when compared with the controls. Statistics showed there was a significant correlation in sTie-2 results between the two methods (r=0.923, P<0.01). The result of this study showed that the level of sTie-2 increased in AMI, and OPC-IE assay was a fast, reliable, and convenient technique to measure sTie-2 in serum.
Wang
Chun-Ling
CL
Heart Centre, China Mei-tan General Hospital, Beijing, China. wangchlcams@yahoo.com.cn
Li
Jun-Qiu
JQ
Li
Hong-Wei
HW
Jin
Gang
G
Wang
Zhan-Hui
ZH
Meng
Yong-Hong
YH
Xiu
Rui-Juan
RJ
D000818:Animals; D015374:Biosensing Techniques; D004797:Enzyme-Linked Immunosorbent Assay; D005240:Feasibility Studies; D006801:Humans; D051379:Mice; D009203:Myocardial Infarction / Q000097:blood* / Q000175:diagnosis*; D055620:Optical Phenomena; D040081:Protein Array Analysis / Q000379:methods*; D042787:Receptor, TIE-2 / Q000097:blood* / Q000737:chemistry*; D012995:Solubility
D042787:Receptor, TIE-2