title: Early modification of c-myc, Ha-ras and p53 expressions by chemical carcinogens (DMBA, MNU).
abstract: 7,12-Dimethylbenz[a]anthracene (DMBA) and N-methyl-N-nitrosourea (MNU) are important environmental carcinogens. Their different biological effects were examined in CBA/Ca H-2(K) haplotype inbred mice on the gene expression of c-myc, Ha-ras and p53 through a 24 hour period. Elevated expression of c-myc and Ha-ras genes was found in the spleen, lung, thymus and lymph nodes 6 and 12 hours after DMBA treatment and in the lung and thymus 3 hours after MNU treatment. In the liver, DMBA induced strong onco/suppressor gene expression as early as 6 hours after the treatment, but MNU increased the p53 gene expression 12 hours after the treatment. The gene expression patterns reflected the different mechanism of the direct acting MNU and metabolically activated DMBA. This phenomenon provides evidence as to the usefulness of detection of onco/supressor key gene expression as early molecular epidemiological biomarkers of carcinogenesis and carcinogenic exposure in animal model, useful in human cancer prevention practice as well.
authors: Department of Public Health, University Medical School of Pécs, H-7643 Pécs, Hungary.
authors: Ferenc
authors: F
authors: Budán
authors: Tímea
authors: T
authors: Varjas
authors: Ghodratollah
authors: G
authors: Nowrasteh
authors: Ida
authors: I
authors: Prantner
authors: Zsuzsa
authors: Z
authors: Varga
authors: Agoston
authors: A
authors: Ember
authors: József
authors: J
authors: Cseh
authors: Katalin
authors: K
authors: Gombos
authors: Emese
authors: E
authors: Pázsit
authors: Gyula
authors: G
authors: Gobel
authors: Miklós
authors: M
authors: Bauer
authors: Tünde
authors: T
authors: Gracza
authors: István
authors: I
authors: Arany
authors: Pál
authors: P
authors: Perjési
authors: István
authors: I
authors: Ember
authors: István
authors: I
authors: Kiss
mesh_terms: D015127:9,10-Dimethyl-1,2-benzanthracene / Q000737:chemistry / Q000633:toxicity*; D000477:Alkylating Agents / Q000737:chemistry / Q000633:toxicity; D000818:Animals; D002273:Carcinogens / Q000737:chemistry / Q000633:toxicity*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D008297:Male; D008770:Methylnitrosourea / Q000737:chemistry / Q000633:toxicity; D051379:Mice; D008808:Mice, Inbred CBA; D016271:Proto-Oncogene Proteins c-myc / Q000235:genetics*; D016283:Proto-Oncogene Proteins p21(ras) / Q000235:genetics*; D016159:Tumor Suppressor Protein p53 / Q000235:genetics*
chemical_list: D000477:Alkylating Agents; D002273:Carcinogens; C489428:Myc protein, mouse; D016271:Proto-Oncogene Proteins c-myc; D016159:Tumor Suppressor Protein p53; D015127:9,10-Dimethyl-1,2-benzanthracene; D008770:Methylnitrosourea; D016283:Proto-Oncogene Proteins p21(ras)