Characterization of start-up performance and archaeal community shifts during anaerobic self-degradation of waste-activated sludge.
Successful start-up strategy for anaerobic digestion of waste-activated sludge using internal inoculum and relationship between the shift of methanogenic community and the digester performance during start-up was investigated. Combination of TS control of inoculum and batch operation during early days enabled the successful start-up operation without serious volatile fatty acid accumulation, followed by the stable continuous operation. However, the propionate degradation was rate-limiting step during the batch operation. The results of real-time quantitative polymerase chain reaction analysis suggested that there was a correlation between the population of the genus Methanosarcina and the methane production rate coupled with acetate consumption during batch operation, and the results of terminal-restriction fragment length polymorphism (T-RFLP) revealed that the increasing intensity of T-RF peaks of hydrogenotrophic methanogens was associated with a decrease in the level of C3-acids.
Kobayashi
Takuro
T
Department of Civil and Environmental Engineering, Tohoku University, 6-6-06 Aza-Aoba, Aramaki, Aoba-ku, Sendai, Miyagi 980-8579, Japan.
Yasuda
Daisuke
D
Li
Yu-You
YY
Kubota
Kengo
K
Harada
Hideki
H
Yu
Han-Qing
HQ
D000693:Anaerobiosis; D001105:Archaea / Q000235:genetics / Q000378:metabolism*; D001673:Biodegradation, Environmental; D019149:Bioreactors / Q000382:microbiology; D002999:Clone Cells; D005232:Fatty Acids, Volatile / Q000032:analysis; D008697:Methane / Q000378:metabolism; D010802:Phylogeny; D012150:Polymorphism, Restriction Fragment Length; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012722:Sewage / Q000382:microbiology*; D013997:Time Factors; D014866:Waste Products / Q000032:analysis*
D005232:Fatty Acids, Volatile; D012336:RNA, Ribosomal, 16S; D012722:Sewage; D014866:Waste Products; D008697:Methane