Effect of using a stand-off pad on Campylobacter jejuni strain diversity in a herd of dairy cows.
OBJECTIVE
To determine the effect of stand-off pad (SOP) use on the prevalence and strain diversity of Campylobacter jejuni in a small herd of dairy cows.


RESULTS
Faecal samples were collected from 21 cows on four sampling occasions (events), one in each season, over 1 year. The cows usually grazed on pasture but during winter they spent 18 h a day on a SOP. Campylobacter prevalence ranged from 48-52% on pasture but was 62% on the SOP. The diversity of 386 C. jejuni isolates was determined using Enterobacterial Repetitive Intergenic Consensus polymerase chain reaction (ERIC/PCR). There were 11 ERIC types identified for the herd over the course of the study. Of those 11, four to seven (per event) were present when the cows were grazing pasture but only two during SOP use.


CONCLUSIONS
The use of the SOP was associated with an increase in prevalence and a reduction in diversity of C. jejuni.


CONCLUSIONS
The reduction in ERIC types on the SOP indicated an increase in transfer of only some strains of C. jejuni among the cows. One of these strains persisted throughout the study. The zoonotic potential of this strain warrants further investigation.
Ross
C M
CM
AgResearch, Hamilton, New Zealand.
Donnison
A M
AM
Clark
D A
DA
D000818:Animals; D016123:Campylobacter jejuni / Q000235:genetics / Q000302:isolation & purification*; D002417:Cattle / Q000382:microbiology*; D005260:Female; D016133:Polymerase Chain Reaction; D015203:Reproducibility of Results