[Research on the evaluation methods for antioxidant capacity of several bioactive substances in vitro].
OBJECTIVE
To study the evaluation methods for antioxidant capacity of bioactive substance in vitro, and to apply the methods which is established in physicochemical environment in order to give the preliminary assessment of test subjects.


METHODS
The combined application of ABTS and FRAP methods in the assessments, using the UV-VIS spectrophotometer and the autoanalyzer respectively test the antioxidant capacity of quercetin, curcumin, DL-alpha-tocopherol and procyanidine at 734 nm in ABTS and at 595 nm in FRAP.


RESULTS
ABTS: The TEAC values of quercetin and curcumin were about 2.02 and 0.50. 1 g DL-alpha-tocopherol and anthocyanins were equivalent to 2.06 mmol, 2.897 mmol of Trolox in scavenging free radicals capacity. FRAP: Used 1.0 mmol/L FeSO4 as the reference standard, quercetin, curcumin and Trolox equivalent molar about 5.73, 1.18 and 2.09. DL-alpha-tocopherol, antioxidant activity of proanthocyanidins were 207.7mg and 156.36 mg.


CONCLUSIONS
It was supported support that combined ABTS and FRAP methods, because of their conveniently, and their reliable results, especially in appropriately be used as the evaluation methods for antioxidant capacity of bioactive substance in vitro.
Liu
Xiaobing
X
Institute of Nutrition and Food Safety, Chinese Center for Disease Control and Prevention, Beijing 100050, China.
Piao
Jianhua
J
Tian
Yuan
Y
D000975:Antioxidants / Q000737:chemistry*; D052160:Benzothiazoles / Q000737:chemistry; D001681:Biological Assay; D003474:Curcumin / Q000737:chemistry; D005290:Ferric Compounds / Q000737:chemistry; D016166:Free Radical Scavengers / Q000737:chemistry*; D006801:Humans; D010936:Plant Extracts / Q000737:chemistry*; D011794:Quercetin / Q000737:chemistry; D013053:Spectrophotometry / Q000379:methods; D013451:Sulfonic Acids / Q000737:chemistry; D024502:alpha-Tocopherol / Q000737:chemistry
D000975:Antioxidants; D052160:Benzothiazoles; D005290:Ferric Compounds; D016166:Free Radical Scavengers; D010936:Plant Extracts; D013451:Sulfonic Acids; C002502:2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid; D011794:Quercetin; D024502:alpha-Tocopherol; D003474:Curcumin