title: [Study on repair of full-thickness skin defect with collagen-chitosan dermal stent in pigs].
abstract: OBJECTIVE
  To investigate angiogenesis of collagen-chitosan porous scaffold, and to study survive of skin grafts on the scaffold after bilayer dermal equivalent (BDE) was transplanted on wounds with full thickness skin defects.
  
  
  METHODS
  The full thickness skin defects were made on 10 Bama miniature pigs and the BDE composed of collagen-chitosan porous scaffold and silicone membrane was transplanted on wound. Angiogenesis in dermal equivalent, wound healing, and healing and survive of skin grafts on dermal equivalent were observed in 1, 2, and 3 weeks after the BDE transplantation. At the same time, CD34 positive signals (neo-forming micro-vessels) were detected by immunohistochemical staining.
  
  
  RESULTS
  Inflammatory cells and fibroblasts infiltrated into dermal equivalent and a few new micro-vessels had been formed in 1 week after the BDE transplantation; neo-forming micro-vessels perpendicular to wound bed had increased significantly in 2 weeks after the BDE transplantation; neo-forming micro-vessels could be observed in almost all dermal equivalents in 3 weeks after the BDE transplantation. CD34 positive signals (neo-forming micro-vessels) in 3 weeks after the BDE transplantation was much more than those in 2 weeks after the BDE transplantation, and CD34 positive signals in 2 weeks after the BDE transplantation was much more than those in 1 week after the BDE transplantation. Survival rate of intermediate split thickness skin graft were 10%, 70% and 100% respectively after the skin grafts had been grafted for 2 weeks on surface of the scaffold which had been transplanted for 1, 2 and 3 weeks. Epidermis which had been grafted on surface of the scaffold for 1 or 2 weeks could perfectly survive after BDE had been transplanted for 1 or 2 weeks.
  
  
  CONCLUSIONS
  Collagen-chitosan porous scaffold plays a very important role in wound healing of full thickness skin defect and can induce fibroblast infiltration and new micro-vessel formation. Epidermis grafted on surface of collagen-chitosan porous scaffold can perfectly repair wounds, and it has brilliant applied prospects in repairing skin defect.
authors: Department of Plastic Surgery, the Affiliated Hospital of Hangzhou Normal University, Hangzhou 310015, China. shaojunxu5@yahoo.com.cn
authors: Shao-Jun
authors: SJ
authors: Xu
authors: Ai-Bin
authors: AB
authors: Huang
authors: Lie
authors: L
authors: Ma
authors: Jian-Ying
authors: JY
authors: Teng
authors: You-di
authors: YD
authors: Ni
authors: Chang-You
authors: CY
authors: Gao
authors: Zhi-Liang
authors: ZL
authors: Zhang
authors: Qing-Hua
authors: QH
authors: DU
authors: Zheng-Cheng
authors: ZC
authors: Dong
mesh_terms: D000818:Animals; D048271:Chitosan; D003094:Collagen; D004195:Disease Models, Animal; D005260:Female; D006085:Graft Survival; D018919:Neovascularization, Physiologic; D012828:Silicones; D012867:Skin / Q000293:injuries; D016038:Skin Transplantation; D013552:Swine; D013556:Swine, Miniature; D054457:Tissue Scaffolds; D014945:Wound Healing
chemical_list: D012828:Silicones; D003094:Collagen; D048271:Chitosan