[Effect on bioactive components of Xuesaitong injection in the process of ultra-filter].
OBJECTIVE
To study the effect on bioactive components of Xuesaitong injection in the process of ultra-filter by orthogonal test.


METHODS
The change of the contents of active components was examined by HPLC method, using notoginsennoside R1 , ginsennoside Rg1, ginsennoside Rb1 and ginsennoside Rd as the mark components. The effect of the transmissivity of active components was studied with four different membrane parameters (such as pore diameter of membrane, operational pressure, the temperature and the concentration of the officinal liquid) by orthogonal test.


RESULTS
In the process of ultra-filter the transmissivity of notoginsennoside R, and ginsennoside Rg, had the same characteristics. Both of the average transmissivities of these two components were in the range of 45% to 95%. The concentration of the officinal liquid showed significant influence. The transmissivities of ginsennoside Rb, and ginsennoside Rd in the process of ultra-filiter had the same characteristic. The average transmissivities of these two components were in the range of 2 8%-62% and 1.8%-45%, respectively. The pore diameter of membrane and the temperature showed significant influence. The concentration of the officinal liquid also showed significant influence on the transmissivity of ginsennoside Rd.


CONCLUSIONS
This study provides an external direction for the preparation of Xuesaitong injection.
Xiong
Hai-Wei
HW
Research Institute of Chinese Medicine, Nanjing University of Traditional Chinese Medicine, Nanjing 210029, China.
Peng
Guo-Ping
GP
Wen
Hong-Mei
HM
Guo
Ke-Jia
KJ
Wang
Xin-Zhi
XZ
Guo
Li-Wei
LW
D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000302:isolation & purification; D036145:Ginsenosides / Q000032:analysis*; D053723:Panax notoginseng / Q000737:chemistry*; D018517:Plant Roots / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry; D011786:Quality Control; D012503:Saponins / Q000737:chemistry* / Q000302:isolation & purification*; D013678:Technology, Pharmaceutical / Q000379:methods*; D014462:Ultrafiltration
D004365:Drugs, Chinese Herbal; D036145:Ginsenosides; D012503:Saponins; C509799:xuesetong; C035054:ginsenoside Rg1