Combining electron crystallography and X-ray crystallography to study the MlotiK1 cyclic nucleotide-regulated potassium channel.
We have recently reported the X-ray structure of the cyclic nucleotide-regulated potassium channel, MlotiK1. Here we describe the application of both electron and X-ray crystallography to obtain high quality crystals. We suggest that the combined application of these techniques provides a useful strategy for membrane protein structure determination. We also present negative stain projection and cryo-data projection maps. These maps provide new insights about the properties of the MlotiK1 channel. In particular, a comparison of a 9A cryo-data projection with calculated model maps strongly suggests that there is a very weak interaction between the pore and the S1-S4 domains of this 6 TM tetrameric cation channel and that the S1-S4 domains can adopt multiple orientations relative to the pore.
Clayton
Gina M
GM
Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT 06520, USA. gina_clayton@merck.com
Aller
Steve G
SG
Wang
Jimin
J
Unger
Vinzenz
V
Morais-Cabral
João H
JH
D003461:Crystallography; D018360:Crystallography, X-Ray; D004583:Electrons; D008958:Models, Molecular; D015221:Potassium Channels / Q000737:chemistry*; D011487:Protein Conformation
D015221:Potassium Channels