Recombinase-mediated cassette exchange provides a versatile platform for gene targeting: knockout of miR-31b.
A series of vectors has been designed to enhance the versatility of targeted homologous recombination. Recombinase-mediated cassette exchange permits sequential targeting at any locus and improves flexibility in making user-defined mutations. Application of RMCE to delete an intronic microRNA gene is described.
Weng
Ruifen
R
Temasek Life Sciences Laboratory, National University of Singapore, 117604, Republic of Singapore.
Chen
Ya-Wen
YW
Bushati
Natascha
N
Cliffe
Adam
A
Cohen
Stephen M
SM
D000818:Animals; D030801:Animals, Genetically Modified; D004331:Drosophila melanogaster / Q000235:genetics; D018390:Gene Targeting / Q000379:methods*; D018014:Gene Transfer Techniques; D005822:Genetic Vectors / Q000138:chemical synthesis / Q000235:genetics; D035683:MicroRNAs / Q000235:genetics*; D008954:Models, Biological; D016254:Mutagenesis, Insertional / Q000379:methods*; D045522:Recombinases / Q000502:physiology*; D011995:Recombination, Genetic
D035683:MicroRNAs; D045522:Recombinases