title: Electrochemical behavior of lactate dehydrogenase immobilized on "silica sol-gel/nanometre-sized tridecameric aluminum polycation" modified gold electrode and its application.
abstract: This paper reports the electrochemical behavior of lactate dehydrogenase (LDH) immobilized in the silica sol-gel film on gold electrode after adding nanometre-sized tridecameric aluminium polycation (nano-Al13, also called nanopolynuclear Al13) as a promoter. A pair of surface controlled quasi-reversible cyclic voltammetry peaks with the formal potential (E0') of 154 mV (vs. SCE) was found in the presence of nano-Al13. A potential application of the nano-Al13-LDH electrode for the determination of resorcinol and p-xylene was also investigated. The experimental results showed that both resorcinol and p-xylene inhibited LDH activity, and the calibration ranges were 5.0 x 10(-6)-3.0 x 10(-4) mol L(-1) for resorcinol and 1.0 x 10(-6)-1.0 x 10(-5) mol L(-1) for p-xylene, respectively. The nano-Al13-LDH electrode can be anticipated to be applied to environmental toxic assessments.
authors: School of Chemistry & Chemical Engineering, State Key Laboratory of Coordination Chemistry of China & Key Laboratory of MOE for Life Science, Nanjing University, Nanjing, 210093, People's Republic of China.
authors: Jiongjia
authors: J
authors: Cheng
authors: Deqian
authors: D
authors: Huang
authors: Jing
authors: J
authors: Zhang
authors: Wenjing
authors: W
authors: Yang
authors: Na
authors: N
authors: Wang
authors: Yongbo
authors: Y
authors: Sun
authors: Keyu
authors: K
authors: Wang
authors: Xiangyin
authors: X
authors: Mo
authors: Shuping
authors: S
authors: Bi
mesh_terms: D000535:Aluminum / Q000737:chemistry*; D000818:Animals; D015374:Biosensing Techniques; D002417:Cattle; D004563:Electrochemistry; D004566:Electrodes; D004785:Environmental Pollutants / Q000032:analysis; D004800:Enzymes, Immobilized / Q000737:chemistry* / Q000378:metabolism; D005782:Gels; D006046:Gold / Q000737:chemistry*; D007770:L-Lactate Dehydrogenase / Q000737:chemistry* / Q000378:metabolism; D053768:Metal Nanoparticles / Q000737:chemistry*; D012822:Silicon Dioxide / Q000737:chemistry*
chemical_list: D004785:Environmental Pollutants; D004800:Enzymes, Immobilized; D005782:Gels; D006046:Gold; D012822:Silicon Dioxide; D000535:Aluminum; D007770:L-Lactate Dehydrogenase