title: [Effects of pigment epithelial derived factor gene on growth of lung cancer cell and neovascularization: experiments with lung cancer cells and chick embryos].
abstract: OBJECTIVE
  To investigate the effect of pigment epithelium derived factor (PEDF) on the growth of lung cancer cells and the cancer-related neovascularization.
  
  
  METHODS
  The full length of human PEDF gene was amplified by polymerase chain reaction (PCR). Human lung cancer cells of the line SKEMS1 were cultured and transfected with PEDF(exp), An eukaryotic expression vector constructed by recombinant DNA technology, so as to construct the SKMES1(PEDFexp) cells over-expressing PEDF protein. RT-PCR and Western blotting were used to confirm the mRNA and protein expression of PEDF in these cells. Another SKMES1 lung cancer cells were transfected with blank plasmids (SKMES1(pEF/His) cells). The SKMES1 cells not transfected were called SKMES1(WT) cells. The 3 kinds of SKMES1 cells were inoculated in the chorio-allantoic membrane (CAM) of chick embryo hatched for 7 days respectively. The size and weigh of the tumor were measured. The vessels density was examined.
  
  
  RESULTS
  The tumor volume of the SKMES1(PEDFexp) group was (0.10 +/- 0.05) cm(3), significantly smaller than that of the control group [(0.17 +/- 0.07) cm(3), P = 0.016], and the mass of the SKME(SPEDFexp) group was (0.008 +/- 0.004) mg, significantly smaller than that of the control group too [(0.024 +/- 0.009) mg, P = 0.006]. The amount of first class neo-vessels of the SKMES1(PEDFexp) group was (15 +/- 3), significantly fewer than that of the control group [(41 +/- 9), P < 0.001]. The amount of second class neo-vessels of the SKMES1(PEDFexp) group was (75 +/- 22), also significantly fewer than that of the control group [(175 +/- 39), P = 0.001].
  
  
  CONCLUSIONS
  Inhibiting the growth of lung cancer cells and neovascularization, PEDF protein may be used as a potential biological drug to treat lung cancer.
authors: Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education), Department of Thoracic Surgery, Peking University School of Oncology, Beijing Cancer Hospital & Institute, Beijing 100142, China.
authors: Jin-Feng
authors: JF
authors: Chen
authors: Wei
authors: W
authors: Zhao
authors: Jian-Zhi
authors: JZ
authors: Zhang
authors: Wen G
authors: WG
authors: Jiang
authors: Li-Jian
authors: LJ
authors: Zhang
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D002642:Chick Embryo; D005136:Eye Proteins / Q000235:genetics*; D015870:Gene Expression; D005822:Genetic Vectors; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics / Q000473:pathology*; D009389:Neovascularization, Pathologic; D009414:Nerve Growth Factors / Q000235:genetics*; D010957:Plasmids; D015843:Serpins / Q000235:genetics*; D014162:Transfection; D000099174:Pigment Epithelium-Derived Factor
chemical_list: D005136:Eye Proteins; D009414:Nerve Growth Factors; D015843:Serpins; D000099174:Pigment Epithelium-Derived Factor