Antioxidant probucol attenuates myocardial oxidative stress and collagen expressions in post-myocardial infarction rats.
This study was designed to evaluate the effects of the antioxidant probucol on myocardial oxidative stress and collagen remodeling by determining type I and III collagen together with relevant collagen mRNA expressions in both the infarcted and noninfarcted myocardium in post-myocardial infarction (MI) rats. Acute myocardial infarction was induced by ligation of the left anterior coronary artery in rats. Rats surviving 24 h after MI were randomly assigned to the group treated with vehicle or probucol. Sham-operated rats served as controls. Cardiac hemodynamics, parameters of oxidative stress in noninfarcted myocardium, collagen content, collagen volume density fraction, collagen type I and III together with the ratio, type I and III collagen mRNA were evaluated after 6 weeks. Probucol decreased oxidative stress as assessed by increased myocardial total antioxidative capacity, superoxide dismutase (SOD) activity, and SOD-to-myocardial malondialdehyde (MDA) ratio accompanied by decreased MDA level, decreased left ventricular end diastolic pressure and LV -dP/dtmax, and decreased collagen content and CVF in the noninfarcted area accompanied by decrease of type I and III mRNA expressions. The increase of collagen type I/III ratio in noninfarcted area was suppressed by probucol accompanied by inhibition of the increase in type I/III collagen mRNA ratio. Probucol did not affect collagen type I/III ratio and the corresponding mRNA ratio in the infarcted area. These results suggest that suppression of oxidative stress by probucol may attenuate collagen synthesis by inhibition of collagen mRNA expressions and improve diastolic function.
Zhou
Shu-xian
SX
Division of Cardiology, Department of Internal Medicine, Second Affiliated Hospital of Sun Yat-Sen University, Guangzhou 510120, China. zzhoushuxian@yahoo.com.cn
Zhou
Yan
Y
Zhang
Yu-ling
YL
Lei
Juan
J
Wang
Jing-feng
JF
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D003094:Collagen / Q000096:biosynthesis / Q000187:drug effects / Q000378:metabolism; D024042:Collagen Type I / Q000187:drug effects / Q000235:genetics; D024061:Collagen Type III / Q000187:drug effects / Q000235:genetics; D003971:Diastole / Q000187:drug effects; D004195:Disease Models, Animal; D005786:Gene Expression Regulation / Q000187:drug effects; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D009203:Myocardial Infarction / Q000188:drug therapy* / Q000503:physiopathology; D009206:Myocardium / Q000473:pathology; D018384:Oxidative Stress / Q000187:drug effects*; D011341:Probucol / Q000494:pharmacology*; D012333:RNA, Messenger / Q000187:drug effects / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D013482:Superoxide Dismutase / Q000187:drug effects / Q000378:metabolism
D000975:Antioxidants; D024042:Collagen Type I; D024061:Collagen Type III; D012333:RNA, Messenger; D008315:Malondialdehyde; D003094:Collagen; D013482:Superoxide Dismutase; D011341:Probucol