title: Metallo-beta-lactamase-producing Pseudomonas spp. in Korea: high prevalence of isolates with VIM-2 type and emergence of isolates with IMP-1 type.
abstract: OBJECTIVE
  Two Korean nationwide studies showed that metallo-beta-lactamases (MBLs)-producing-Pseudomonas spp. are not rare. The aim of this study was to assess the trends of MBL-producing isolates among imipenem-resistant isolates of Pseudomonas spp.
  
  
  METHODS
  Imipenem-resistant clinical isolates were collected from 23 hospitals and one commercial laboratory participating in the KONSAR program in 2005. Polymerase chain reaction (PCR) was used to detect MBL genes.
  
  
  RESULTS
  Alleles of MBL genes were detected in 10.8% of 415 Pseudomonas aeruginosa and 66.7% of 12 P. putida isolates from 18 of 24 hospitals/laboratory. Among the 14 IMP-1-like and 39 VIM-2-like MBLs, emergence of IMP-6 was detected for the first time.
  
  
  CONCLUSIONS
  Prevalence of MBL-producing P. aeruginosa has not significantly increased, but IMP-6 emerged in P. aeruginosa.
authors: Department of Laboratory Medicine, Yonsei University College of Medicine, 250 Seongsan-ro, Seodaemun-gu,Seoul, Korea.
authors: Kyungwon
authors: K
authors: Lee
authors: Ae Ja
authors: AJ
authors: Park
authors: Moon Yeun
authors: MY
authors: Kim
authors: Hee Joo
authors: HJ
authors: Lee
authors: Ji-Hyun
authors: JH
authors: Cho
authors: Jung Oak
authors: JO
authors: Kang
authors: Dongeun
authors: D
authors: Yong
authors: Yunsop
authors: Y
authors: Chong
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D016521:Electrophoresis, Gel, Pulsed-Field; D006801:Humans; D015378:Imipenem / Q000494:pharmacology; D007723:Korea; D016133:Polymerase Chain Reaction; D011552:Pseudomonas Infections / Q000382:microbiology*; D011550:Pseudomonas aeruginosa / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D001618:beta-Lactamases / Q000235:genetics / Q000378:metabolism*
chemical_list: D000900:Anti-Bacterial Agents; D015378:Imipenem; C409201:beta-lactamase IMP-1; C425108:beta-lactamase bla(vim-2); D001618:beta-Lactamases