The effect of beta interferon on dendritic cells and cytokine synthesis by CD4+ T cells.
BACKGROUND
Dendritic cells (DC) are a key regulator of the immune response, and interferon-beta (IFN-beta) is considered an immunomodulatory molecule for DC.


OBJECTIVE
The purpose of this study was to evaluate the ability of IFN-beta treated DC to induce cytokine secretion by CD4+ T cells.


METHODS
Dendritic cells were generated from blood monocytes with granulocyte-monocyte colony-stimulating factor and interleukin-4 with or without IFN-beta. We analyzed the production of CD4+ T helper cytokines (IL-17, IFN-gamma and IL-10) in the supernatant of the dendritic cell-T cell co- cultures by ELISA. We also studied the effects of HLA-G and costimulatory molecules on immature and mature DC.


RESULTS
IFN-gamma and IL-17 decreased significantly in the presence of HLA-G-bearing DC compared to control cultures (p<0.05).


CONCLUSIONS
Using the mixed leukocyte reaction, we found that DC treated with IFN-beta mediated the inhibition of T cell activation via cytokine production. We conclude that this is important for preventing overactivation of the immune system.
Abediankenari
Saeid
S
Department of Microbiology and Immunology, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran. abedianlab@yahoo.co.uk
Shaker
Davoud
D
Abedian
Farshideh
F
Mirabi
Arazmohammad
A
D015496:CD4-Positive T-Lymphocytes / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D002450:Cell Communication / Q000276:immunology; D002454:Cell Differentiation; D018920:Coculture Techniques; D003713:Dendritic Cells / Q000187:drug effects / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D004797:Enzyme-Linked Immunosorbent Assay; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000276:immunology / Q000378:metabolism; D006680:HLA Antigens / Q000378:metabolism; D059951:HLA-G Antigens; D015395:Histocompatibility Antigens Class I / Q000378:metabolism; D006801:Humans; D016899:Interferon-beta / Q000276:immunology / Q000378:metabolism* / Q000494:pharmacology; D007371:Interferon-gamma / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D007378:Interleukins / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D008213:Lymphocyte Activation / Q000187:drug effects; D007959:Lymphocyte Culture Test, Mixed; D009000:Monocytes / Q000473:pathology
D006680:HLA Antigens; D059951:HLA-G Antigens; D015395:Histocompatibility Antigens Class I; D007378:Interleukins; D016899:Interferon-beta; D007371:Interferon-gamma; D016178:Granulocyte-Macrophage Colony-Stimulating Factor