Separation of oligonucleotides with single-base mutation by capillary electrophoresis using specific interaction of metal ion with nucleotide.
A unique tactic for the separation of single-base sequential isomers of oligomeric single-stranded DNA by a CE separation system employing the specific interaction of metal ion with nucleotide was demonstrated, enabling the separation of the mixture of a 12-mer oligonucleotide and its single-base mutants, as well as their positional isomers.
Takahashi
Toru
T
Division of Environmentally Benign Systems, Graduate School of Environmental Studies, Tohoku University, 20 Aoba, Aramaki, Aoba-ku, Sendai 980-8579, Japan. toru@analchem.che.tohoku.ac.jp
Sakurai
Takao
T
Hirata
Koichiro
K
Hoshino
Hitoshi
H
D001483:Base Sequence; D004277:DNA, Single-Stranded / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D019075:Electrophoresis, Capillary; D007536:Isomerism; D008670:Metals / Q000737:chemistry*; D009154:Mutation; D009841:Oligonucleotides / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification*; D013379:Substrate Specificity
D004277:DNA, Single-Stranded; D008670:Metals; D009841:Oligonucleotides