title: Electroacoustic polymer microchip as an alternative to quartz crystal microbalance for biosensor development.
abstract: Laser photoablation of poly(ethylene terephthalate) (PET), a flexible dielectric organic polymer, was used to design an acoustic miniaturized DNA biosensor. The microchip device includes a 100-microm-thick PET layer, with two microband electrodes patterned in photoablated microchannels on one side and a depressed photoablated disk decorated by gold sputtered layer on the other side. Upon application of an electric signal between the two electrodes, an electroacoustic resonance phenomenon at approximately 30 MHz was established through the microelectrodes/PET/ gold layer interface. The electroacoustic resonance response was fitted with a series RLC motional arm in parallel with a static Co arm of a Buttlerworth-Van Dyke equivalent circuit: admittance spectra recorded after successive cycles of DNA hybridization on the gold surface showed reproducible changes on R, L, and C parameters. The same hybridizations runs were performed concomitantly on a 27-MHz (9 MHz, third overtone) quartz crystal microbalance in order to validate the PET device developed for bioanalysis applications. The electroacoustic PET device, approximately 100 times smaller than a microbalance quartz crystal, is interesting for the large-scale integration of acoustic sensors in biochips.
authors: Laboratoire Interfaces et Systèmes Electrochimiques, CNRS UPR15-LISE, Université Pierre et Marie Curie, 4 Place Jussieu, Paris F-75005, France. jean.gamby@upmc.fr
authors: Jean
authors: J
authors: Gamby
authors: Mathieu
authors: M
authors: Lazerges
authors: Hubert H
authors: HH
authors: Girault
authors: Claude
authors: C
authors: Deslouis
authors: Claude
authors: C
authors: Gabrielli
authors: Hubert
authors: H
authors: Perrot
authors: Bernard
authors: B
authors: Tribollet
mesh_terms: D000162:Acoustics / Q000295:instrumentation*; D001483:Base Sequence; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D048028:DNA, Algal / Q000032:analysis*; D004563:Electrochemistry / Q000295:instrumentation / Q000379:methods; D004867:Equipment Design; D056890:Eukaryota / Q000235:genetics*; D006046:Gold / Q000737:chemistry; D009693:Nucleic Acid Hybridization / Q000379:methods*; D011093:Polyethylene Terephthalates / Q000737:chemistry*; D011791:Quartz / Q000737:chemistry; D015203:Reproducibility of Results; D013499:Surface Properties
chemical_list: D048028:DNA, Algal; D011093:Polyethylene Terephthalates; D011791:Quartz; D006046:Gold