Two-parameter monitoring in a lab-on-valve manifold, applied to intracellular H2O2 measurements.
This work introduces, for the first time, simultaneous monitoring of fluorescence and absorbance using Bead Injection in a Lab-on-valve format. The aim of the paper is to show that when the target species, cells immobilized on a stationary phase, are exposed to reagents under well-controlled reaction conditions, dual monitoring yields valuable information. The applicability of this technique is demonstrated by the development of a Bead Injection method for automated measurement of cell density and intracellular hydrogen peroxide.
Lähdesmäki
Ilkka
I
Department of Chemistry, University of Washington, Box 351700, Seattle, WA 98195-1700, USA.
Chocholous
Petr
P
Carroll
Andrea D
AD
Anderson
Judy
J
Rabinovitch
Peter S
PS
Ruzicka
Jaromir
J
D000818:Animals; D016477:Artifacts; D002138:Calibration; D002452:Cell Count; D005347:Fibroblasts / Q000166:cytology; D005456:Fluorescent Dyes / Q000737:chemistry; D006861:Hydrogen Peroxide / Q000032:analysis*; D042541:Intracellular Space / Q000737:chemistry*; D008027:Light; D051379:Mice; D044085:Microfluidics; D013050:Spectrometry, Fluorescence / Q000295:instrumentation / Q000379:methods*
D005456:Fluorescent Dyes; D006861:Hydrogen Peroxide