[Study of ALAD and VDR gene polymorphisms associated with lead nephrotoxicity susceptibility].
OBJECTIVE
To explore lead nephrotoxicity associated with delta-aminolevulinic acid dehydratase, vitamin D receptor gene polymorphism as well as joint effect of gene-gene, gene-environment.


METHODS
233 occupational lead-exposed workers were involved in this study. Then blood lead was detected as lead exposure biomarker. Urinary N-acetyl-beta-D-glucosamindase and beta2-microglobin of those workers were measured as biomarkers of nephrotoxicity. PCR-RFLP method was used for gene polymorphism analysing.


RESULTS
Lead exposure concentration was higher than national limit, NAG acticity were (2.12 +/- 0.07) U/mmol Cr in workers with ALAD1-2/ALAD2-2 genotype and (1.73 +/- 0.03) U/mmol Cr in workers with ALAD1-1 genotype (P < 0.05). In the same condition, beta2-MG were (20.94 +/- 1.12) microg/mmol Cr in workers with VDR-Bb genotype and (15.28 +/- 0.09) microg/mmol Cr in workers with VDR-bb genotype (P = 0.01). Analysis of logistic regression confirmed that lead exposure and high blood lead as well as combined effect of gene-environment were responsible for lead nephrotoxicity in workers (OR = 6.58, 2.41, 3.01).


CONCLUSIONS
Polymorphisms of ALAD and VDR gene may play an important role in lead nephrotoxicity in high lead-exposed workers. Joint effect of gene-environment could be involved in lead nephrotoxicity in workers.
Wang
Aihong
A
Ningbo Municipal Center For Disease Control and Prevention, Ningbo 315010, China.
Wang
Qunli
Q
Song
Qifa
Q
Xu
Jingye
J
D000118:Acetylglucosaminidase / Q000652:urine; D000328:Adult; D005260:Female; D020022:Genetic Predisposition to Disease; D006801:Humans; D007674:Kidney Diseases / Q000139:chemically induced* / Q000235:genetics; D007854:Lead / Q000097:blood; D007855:Lead Poisoning / Q000235:genetics* / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D016273:Occupational Exposure; D011110:Polymorphism, Genetic; D000623:Porphobilinogen Synthase / Q000235:genetics*; D018167:Receptors, Calcitriol / Q000235:genetics*; D055815:Young Adult; D001613:beta 2-Microglobulin / Q000652:urine
D018167:Receptors, Calcitriol; D001613:beta 2-Microglobulin; D007854:Lead; D000118:Acetylglucosaminidase; D000623:Porphobilinogen Synthase