The positive effects of Midazolam on functional activity of white rat brain cells in conditions of halothane anesthesia.
For the elimination of postoperative complications, which are evident in infants with congenital palatine and upper lip cleft after halothane anesthesia during standard premedication (Atropine, Dimedrol) the usage of agents of benzodiazepine group with anti-hypoxic effects is advised. The latter may modulate the blocked ion channels by neuromediators, which are activated by GABA and GABA receptor function. The neuro-protective ability of halothane is demonstrated. To reveal the mechanisms of positive effects using benzodiazepine group for premedication we have investigated the effects of midazolam of brain cell functional activity of experimental animals (white rats) in conditions of halothane anesthesia. For the estimation of white rat brain cells functional activity the nuclear transcriptional activity was studied (based on the intensity of [14C]-UTF inclusion), also the glutamic acid decarboxilaze activity (GAD65/67) using immunohistochemistry. It is estimated that halothane inhibits the transcription in rat brain cells. During midazolam premedication the halothane inhibitory effect on RNA synthesis is not revealed. After an hour of pseudo-operation halothane also induces proved decrease of quantity of GAD65/67 positive cells in CA3 hippocampal field. At the same time the quantity of similar cells are increased in CA1 field. The increase of GAD65/67 positive cells in CA1 is more evident during midazolam premedication. Based on the data received we can conclude that the positive effect of midazolam results in increase of GAD65/67 positive cells in CA1 hippocampal field.
Vadachkoria
Z
Z
Tbilisi State Medical University, Department of Children and Adult Surgical Stomatology, Tbilisi, Georgia.
Dzidziguri
L
L
Bakuradze
E
E
Dzidziguri
D
D
D018685:Anesthetics, Inhalation / Q000008:administration & dosage / Q000009:adverse effects*; D000818:Animals; D001921:Brain / Q000166:cytology / Q000187:drug effects* / Q000502:physiology; D006221:Halothane / Q000008:administration & dosage / Q000009:adverse effects*; D006993:Hypnotics and Sedatives / Q000008:administration & dosage*; D008874:Midazolam / Q000008:administration & dosage*; D011183:Postoperative Complications / Q000517:prevention & control*; D011292:Premedication; D051381:Rats
D018685:Anesthetics, Inhalation; D006993:Hypnotics and Sedatives; D008874:Midazolam; D006221:Halothane