title: Guided sputum sample collection and culture contamination rates in the diagnosis of pulmonary TB.
abstract: A comparative study to evaluate contamination in cultures of morning sputum samples, comparing those collected at home under currently recommended conditions and those collected under supervision after patient orientation and education. The home and supervised collection groups produced 43 and 76 sputum samples, respectively. The contamination rate was nearly 3-times higher among samples collected at home than among those collected under supervision (37% vs. 13%, p < 0.05; OR = 0.25). The simple educational and hygiene measures described can decrease the contamination rate among sputum samples collected for diagnostic culture.
authors: Universidade Federal do Espírito Santo, Vitória, Brazil. emaciel@ndi.ufes.br
authors: Ethel Leonor Noia
authors: EL
authors: Maciel
authors: Thiago Nascimento do
authors: TN
authors: Prado
authors: Renata Lyrio
authors: RL
authors: Peres
authors: Moises
authors: M
authors: Palaci
authors: John L
authors: JL
authors: Johnson
authors: Reynaldo
authors: R
authors: Dietze
mesh_terms: D000328:Adult; D004866:Equipment Contamination / Q000517:prevention & control; D005260:Female; D006798:Housing; D006801:Humans; D006920:Hygiene; D008297:Male; D013048:Specimen Handling / Q000379:methods*; D013183:Sputum / Q000382:microbiology*; D014397:Tuberculosis, Pulmonary / Q000175:diagnosis*