title: Viral infection of cells in culture detected using infrared microscopy.
abstract: FTIR microscopy has been used to collect spectra for uninfected (mock) Vero cells, and cells that have been infected with herpes simplex virus type 1 (HSV-1) and human adenovirus type 5 (Ad-5). Cells were infected at a multiplicity of infection of 10, and studied at 24 hours post exposure. The spectra for infected samples display many differences compared to the spectra for uninfected samples. To estimate how well the spectra for uninfected and infected samples could be discriminated, we used logistic and partial least squares regression methods. We show that the spectra for HSV-1 and mock infected samples are well differentiated and, for a sensitivity of 95%, we calculate a specificity of 0.999 using partial least squares regression. Spectra for Ad-5 and mock infected samples are also well differentiated. We find that applying our regression models constructed with one data set to a new validating data set still gives very high levels of specificity for a given sensitivity. Spectra for Ad-5 and HSV-1 infected samples are also differentiable. Applying our constructed regression models to new validating data, however, leads to a decrease in the discrimination capability in this instance. If one is simply interested in differentiating spectra associated with uninfected and infected cells, without distinguishing the type of infection, then we show that logistic regression models can break down whereas partial least squares regression models perform well.
authors: Department of Physics and Astronomy, Georgia State University, Atlanta, GA 30303, USA. ghastings@gsu.edu
authors: Gary
authors: G
authors: Hastings
authors: Peter
authors: P
authors: Krug
authors: Ruili
authors: R
authors: Wang
authors: Jing
authors: J
authors: Guo
authors: Hari P
authors: HP
authors: Lamichhane
authors: Tian
authors: T
authors: Tang
authors: Yu-Sheng
authors: YS
authors: Hsu
authors: John
authors: J
authors: Ward
authors: David
authors: D
authors: Katz
authors: Julia
authors: J
authors: Hilliard
mesh_terms: D000260:Adenoviruses, Human / Q000502:physiology*; D000818:Animals; D002477:Cells / Q000821:virology*; D002522:Chlorocebus aethiops; D018259:Herpesvirus 1, Human / Q000502:physiology*; D008853:Microscopy; D017550:Spectroscopy, Fourier Transform Infrared; D013535:Suspensions; D014709:Vero Cells
chemical_list: D013535:Suspensions