title: The expression of MUC1 in human Fallopian tube during the menstrual cycle and in ectopic pregnancy.
abstract: BACKGROUND
  Ectopic pregnancy is a major cause of maternal morbidity and mortality with increasing incidence worldwide. We have investigated whether epithelia from Fallopian tubes (FTs) bearing an ectopic pregnancy differ from normal tubes in expression of MUC1.
  
  
  METHODS
  Since it is not possible to collect FTs from women carrying a healthy pregnancy, we studied tissue collected at the time of hysterectomy for benign disease. Women were injected with hCG in the days leading up to hysterectomy, and pseudopregnancy confirmed by the presence of high serum progesterone levels and the decidualization of the endometrium. FTs from the different stages of the menstrual cycle (n = 24), tubes bearing an ectopic pregnancy (n = 15) and pseudo-pregnant tubes (n = 6) were collected and examined using immunohistochemistry and quantitative RT-PCR.
  
  
  RESULTS
  MUC1 was present at the apical surface of the tubal epithelial cells throughout the menstrual cycle, but intracellular localization was minimal in the follicular phase, increasing to a maximum in the luteal phase. MUC1, including the glycoform recognized by antibody 214D4, was found at the apical surface of tubal epithelium in both the ectopic and pseudo-pregnant groups and the intracellular expression was much stronger in the pseudo-pregnant group than in the ectopic group. The 214D4 epitope was absent from tubal tissue adjacent to ectopic implants.
  
  
  CONCLUSIONS
  The decrease in MUC1 expression and altered glycosylation in tubal epithelium from ectopic pregnancy may reflect an increase in receptivity.
authors: Academic Unit of Reproductive and Developmental Medicine, Level 4, The Jessop Wing, Royal Hallamshire Hospital, Sheffield S10 2SF, UK. alazemimajda@hsc.edu.kw
authors: M
authors: M
authors: Al-Azemi
authors: B
authors: B
authors: Refaat
authors: J
authors: J
authors: Aplin
authors: W
authors: W
authors: Ledger
mesh_terms: D000328:Adult; D005187:Fallopian Tubes / Q000378:metabolism*; D005260:Female; D005498:Follicular Phase / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D006801:Humans; D007150:Immunohistochemistry; D008183:Luteal Phase / Q000235:genetics / Q000378:metabolism; D008597:Menstrual Cycle / Q000235:genetics / Q000378:metabolism*; D018396:Mucin-1 / Q000235:genetics / Q000378:metabolism*; D011247:Pregnancy; D011271:Pregnancy, Ectopic / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism
chemical_list: C060192:MUC1 protein, human; D018396:Mucin-1; D012333:RNA, Messenger