DNA-barcode directed capture and electrochemical metabolic analysis of single mammalian cells on a microelectrode array.
A microdevice is developed for DNA-barcode directed capture of single cells on an array of pH-sensitive microelectrodes for metabolic analysis. Cells are modified with membrane-bound single-stranded DNA, and specific single-cell capture is directed by the complementary strand bound in the sensor area of the iridium oxide pH microelectrodes within a microfluidic channel. This bifunctional microelectrode array is demonstrated for the pH monitoring and differentiation of primary T cells and Jurkat T lymphoma cells. Single Jurkat cells exhibited an extracellular acidification rate of 11 milli-pH min(-1), while primary T cells exhibited only 2 milli-pH min(-1). This system can be used to capture non-adherent cells specifically and to discriminate between visually similar healthy and cancerous cells in a heterogeneous ensemble based on their altered metabolic properties.
Douglas
Erik S
ES
UCSF/UC Berkeley Joint Graduate Group in Bioengineering, University of California, Berkeley, California 94720, USA. ramathies@berkeley.edu
Hsiao
Sonny C
SC
Onoe
Hiroaki
H
Bertozzi
Carolyn R
CR
Francis
Matthew B
MB
Mathies
Richard A
RA
D000818:Animals; D015374:Biosensing Techniques; D004247:DNA / Q000378:metabolism*; D004563:Electrochemistry; D001330:Electronic Data Processing; D006801:Humans; D006863:Hydrogen-Ion Concentration; D019169:Jurkat Cells; D008839:Microelectrodes; D008856:Microscopy, Fluorescence; D013601:T-Lymphocytes / Q000166:cytology / Q000378:metabolism* / Q000473:pathology
D004247:DNA