title: Molecular and biological characterization of natural variants of Citrus dwarfing viroid.
abstract: Citrus dwarfing viroid has been proposed as an agent to control tree size in high-density plantations. Thirty-three field isolates have been characterized, and the most frequent sequence/s have been identified. Five distinct variants were selected for biological characterization. Symptom expression analysis demonstrated a good correlation between leaf/stem symptoms and plant growth. The discriminating nucleotide sequence differences included two deletions and an insertion resulting in a reorganization of the base pairing of the terminal left loop, two (G42 --> A and C52 --> U) changes found in one of the variants, and as many as thirteen changes located in the right and left regions flanking the CCR.
authors: Departamento de Protección Vegetal y Biotecnología, Instituto Valenciano de Investigaciones Agrarias, Centro de Protección Vegetal y Biotecnología, Apartado oficial, 46113 Moncada, Valencia, Spain.
authors: N
authors: N
authors: Murcia
authors: L
authors: L
authors: Bernad
authors: P
authors: P
authors: Serra
authors: S M
authors: SM
authors: Bani Hashemian
authors: Núria
authors: N
authors: Duran-Vila
mesh_terms: D002957:Citrus / Q000254:growth & development / Q000821:virology*; D014644:Genetic Variation; D010935:Plant Diseases / Q000821:virology*; D018515:Plant Leaves / Q000254:growth & development / Q000821:virology; D018547:Plant Stems / Q000254:growth & development / Q000821:virology; D014772:Viroids / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity*