title: Differential methylation pattern of ID4, SFRP1, and SHP1 between acute myeloid leukemia and chronic myeloid leukemia.
abstract: To gain insight into the differential mechanism of gene promoter hypermethylation in acute and chronic leukemia, we identified the methylation status on one part of 5'CpG rich region of 8 genes, DAB2IP, DLC-1, H-cadherin, ID4, Integrin alpha4, RUNX3, SFRP1, and SHP1 in bone marrows from acute myeloid leukemia (AML) and chronic myeloid leukemia (CML) patients. Also, we compared the methylation status of genes in AML and CML using methylation-specific PCR (MSP). The frequencies of DNA methylation of ID4, SFRP1, and SHP1 were higher in AML patients compared to those in CML patients. In contrast, no statistical difference between AML and CML was detected for other genes such as DLC-1, DAB2IP, H-cadherin, Integrin alpha4, and RUNX3. Taken together, these results suggest that these methylation-controlled genes may have different roles in AML and CML, and thus, may act as a biological marker of AML.
authors: Institute of Human Genetics, Department of Anatomy, Brain Korea 21 Biomedical Sciences, Korea University College of Medicine, Seoul, Korea.
authors: Kyung-Ok
authors: KO
authors: Uhm
authors: Eun Soo
authors: ES
authors: Lee
authors: Yun Mi
authors: YM
authors: Lee
authors: Jeong Seon
authors: JS
authors: Park
authors: Seok Jin
authors: SJ
authors: Kim
authors: Byung Soo
authors: BS
authors: Kim
authors: Hyeon Soo
authors: HS
authors: Kim
authors: Sun-Hwa
authors: SH
authors: Park
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D018899:CpG Islands; D019175:DNA Methylation; D005260:Female; D006801:Humans; D051796:Inhibitor of Differentiation Proteins / Q000235:genetics* / Q000378:metabolism; D036341:Intercellular Signaling Peptides and Proteins / Q000235:genetics* / Q000378:metabolism; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000235:genetics / Q000378:metabolism; D015470:Leukemia, Myeloid, Acute / Q000235:genetics* / Q000378:metabolism; D008297:Male; D008565:Membrane Proteins / Q000235:genetics* / Q000378:metabolism; D008875:Middle Aged; D011401:Promoter Regions, Genetic; D053824:Protein Tyrosine Phosphatase, Non-Receptor Type 6 / Q000235:genetics* / Q000378:metabolism; D000094742:Secreted Frizzled-Related Proteins
chemical_list: D051796:Inhibitor of Differentiation Proteins; D036341:Intercellular Signaling Peptides and Proteins; D008565:Membrane Proteins; D053824:Protein Tyrosine Phosphatase, Non-Receptor Type 6; C490128:ID4 protein, human; C117636:SFRP1 protein, human; D000094742:Secreted Frizzled-Related Proteins