Acute homing of bone marrow-derived mononuclear cells in intramyocardial vs. intracoronary transplantation.
OBJECTIVE
Cell homing optimisation after transplantation is critical in myocardial infarction (MI) cell therapy.


METHODS
Eight pigs were randomized to receiving autologous purified (111)indium-labeled bone marrow mononuclear cells (BMMCs) (10(8) cells/2 ml) by intramyocardial (IM) (n=4) or by intracoronary (IC) (n=4) transplantation after 90 minutes occlusion of the CX-coronary artery. Dual isotope SPECT imaging was performed 2 and 24 hours postoperatively. Two animals were additionally analyzed on the sixth postoperative day. Tissue samples from the major organs were analyzed.


RESULTS
In SPECT imaging revealed that BMMCs administered using IM injection remained in the injured area. In contrast, minor proportion of IC transplanted cells remained in the myocardium, as most of the cells showed homing in the lungs. Analysis of the biopsies showed a seven-fold greater number of cells in the myocardium for the IM method and a 10-fold greater number of cells in the lungs in the IC group (p < 0.001).


CONCLUSIONS
In producing persistently high cell homing at the infarction site, the IM transplantation is superior to the IC transplantation. However, the IC administration might be more specific in targeting injured capillaries and epithelial cells within the infarcted myocardium.
Mäkelä
Jussi
J
Department of Surgery, Oulu University Hospital, University of Oulu and Clinical Research Center, 90221, Oulu, Finland.
Anttila
Vesa
V
Ylitalo
Kari
K
Takalo
Reijo
R
Lehtonen
Siri
S
Mäkikallio
Timo
T
Niemelä
Eija
E
Dahlbacka
Sebastian
S
Tikkanen
Jonne
J
Kiviluoma
Kai
K
Juvonen
Tatu
T
Lehenkari
Petri
P
D000818:Animals; D016026:Bone Marrow Transplantation / Q000379:methods*; D002470:Cell Survival / Q000187:drug effects; D018380:Hematopoietic Stem Cell Transplantation / Q000379:methods*; D006412:Hematopoietic Stem Cells / Q000502:physiology*; D007205:Indium Radioisotopes / Q000009:adverse effects; D007261:Infusions, Intra-Arterial; D007273:Injections, Intramuscular; D009203:Myocardial Infarction / Q000000981:diagnostic imaging / Q000601:surgery*; D011877:Radionuclide Imaging; D013552:Swine
D007205:Indium Radioisotopes