[Isolation and identification of arboviruses from mosquito pools in some regions of Liaoning province, China].
OBJECTIVE
To isolate and identify arboviruses from mosquito pools in some regions of Liaoning province.


METHODS
Mosquitoes were collected from Shenyang, Yingkou, Panjin, Jinzhou and Dandong cities of Liaoning province in 2006. Viruses were isolated by inoculating the specimens onto C6/ 36 and BHK-21cells. The new isolates were identified using serological and molecular biological methods.


RESULTS
5410 mosquitoes were collected from the five cities in total. Three isolates produced CPE in C6/ 36 cell and five isolates produced CPE in both C6/36 and BHK-21 cell. Three isolates (LN0684, LN0688 and LN0689) were identified as Banna virus and one isolate (LN0636) was identified as Getah virus. Phylogenetic analysis showed that the three Banna virus strains were clustered into the same evolution branch as the other Chinese isolates. The identity of nucleotide sequence was between 91.2% and 94.7%, compared with other Banna virus strains. The new isolated Getah virus was clustered into the same branch with the strain of South Korea (swine). The identity of nucleotide sequence was 99.2%, when comparing with the strain of South Korea and was 95% to 99% with the strains from Russia, mainland of China and Taiwan region. Conclusion Eight virus isolates, including three Banna virus, one Getah virus and four unknown virus strains were isolated from mosquitoes in Liaoning province. Banna virus and Getah virus were reported for the first time in Liaoning province, while Getah virus showed the highest nucleotide homology with the South Korea strains.
Meng
Wei-Shan
WS
Department of Viral Encephalitis, Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 100052, China.
Zhang
Ji-Bo
JB
Sun
Xiao-Hong
XH
Liu
Qi-Nan
QN
Chen
Zhe
Z
Zhai
You-Gang
YG
Fu
Shi-Hong
SH
Cao
Yu-Xi
YX
Wang
Huan-Yu
HY
Ding
Jun
J
Chu
Fa-Jun
FJ
Li
Zhi
Z
Zhang
Li-Tian
LT
Zhao
Yu-Jun
YJ
Liang
Guo-Dong
GD
D000524:Alphavirus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D000818:Animals; D001103:Arboviruses / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D002460:Cell Line; D002681:China; D018162:Coltivirus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D009033:Culicidae / Q000821:virology*; D010802:Phylogeny; D012367:RNA, Viral / Q000235:genetics; D017422:Sequence Analysis, DNA; D017423:Sequence Analysis, RNA
D012367:RNA, Viral