Chemoselective immobilization of biomolecules through aqueous Diels-Alder and PEG chemistry.
Aqueous Diels-Alder chemistry combined with a poly(ethylene glycol) (PEG) spacer was used to immobilize a diverse group of biomolecules onto a solid surface. Briefly, α, ω linear PEG conjugates were synthesized containing cyclopentadiene in the α position and either biotin, lactose, or protein A in the ω position. Linkers were coupled to N-maleimide (EMC)-functionalized glass substrates, and surface immobilization of biomolecules was confirmed by confocal fluorescence imaging.
Sun
Xue-Long
XL
Department of Chemistry, Cleveland State University, 2121 Euclid Avenue SR 361, Cleveland, OH 44115, United States.
Yang
Liuchun
L
Chaikof
Elliot L
EL