title: Role of oxysterol binding protein in hepatitis C virus infection.
abstract: Hepatitis C virus (HCV) RNA genome replicates within the ribonucleoprotein (RNP) complex in the modified membranous structures extended from endoplasmic reticulum. A proteomic analysis of HCV RNP complexes revealed the association of oxysterol binding protein (OSBP) as one of the components of these complexes. OSBP interacted with the N-terminal domain I of the HCV NS5A protein and colocalized to the Golgi compartment with NS5A. An OSBP-specific short hairpin RNA that partially downregulated OSBP expression resulted in a decrease of the HCV particle release in culture supernatant with little effect on viral RNA replication. The pleckstrin homology (PH) domain located in the N-terminal region of OSBP targeted this protein to the Golgi apparatus. OSBP deletion mutation in the PH (DeltaPH) domain failed to localize to the Golgi apparatus and inhibited the HCV particle release. These studies suggest a possible functional role of OSBP in the HCV maturation process.
authors: Department of Medicine, University of California, San Diego, La Jolla, 92093, USA.
authors: Yutaka
authors: Y
authors: Amako
authors: Ali
authors: A
authors: Sarkeshik
authors: Hak
authors: H
authors: Hotta
authors: John
authors: J
authors: Yates
authors: Aleem
authors: A
authors: Siddiqui
mesh_terms: D045744:Cell Line, Tumor; D006056:Golgi Apparatus / Q000378:metabolism; D016174:Hepacivirus / Q000502:physiology; D006526:Hepatitis C / Q000209:etiology*; D006801:Humans; D011485:Protein Binding; D021381:Protein Transport; D034741:RNA, Small Interfering / Q000494:pharmacology; D011987:Receptors, Steroid / Q000378:metabolism / Q000502:physiology*; D012261:Ribonucleoproteins; D017361:Viral Nonstructural Proteins / Q000378:metabolism*; D014764:Viral Proteins; D014779:Virus Replication; D000098825:Oxysterol Binding Proteins; D012324:RNA-Dependent RNA Polymerase
chemical_list: D034741:RNA, Small Interfering; D011987:Receptors, Steroid; D012261:Ribonucleoproteins; D017361:Viral Nonstructural Proteins; D014764:Viral Proteins; D000098825:Oxysterol Binding Proteins; C087036:NS-5 protein, hepatitis C virus; D012324:RNA-Dependent RNA Polymerase