title: Generation of asparagine-linked glycan structure databases and their use.
abstract: Asparagine linked glycans (N-glycans) are important in biological processes. Yet, their structural complexity and lack of databases hinder progress in glycomics and glycobiology. We present a way for in silico generation of very large N-glycan structure databases and their use in high throughput composition and primary structure determination of N-glycans attached to peptides, based on CID (collision induced dissociation) MS/MS (tandem mass spectrometric data). The database and the integrated search engine is called Glyquest and is available to the glycomics community.
authors: BioPharmaSoft, Boston, Massachusetts 02478, USA. h.gao@biopharmasoft.com
authors: Hui Ying
authors: HY
authors: Gao
mesh_terms: D001216:Asparagine / Q000737:chemistry*; D002240:Carbohydrate Sequence; D003628:Database Management Systems; D016208:Databases, Factual; D054794:Glycomics / Q000379:methods*; D016247:Information Storage and Retrieval / Q000379:methods*; D013058:Mass Spectrometry / Q000379:methods*; D011134:Polysaccharides / Q000737:chemistry*
chemical_list: D011134:Polysaccharides; D001216:Asparagine