title: The dose effect of human bone marrow-derived mesenchymal stem cells on epidermal development in organotypic co-culture.
abstract: BACKGROUND
  A wealth of evidences have shown the participation and benefits of bone marrow-derived mesenchymal stem cells (BM-MSCs) in wound healing and skin tissue repair in vivo. However, their role in epidermal development and reconstitution is not clearly investigated.
  
  
  OBJECTIVE
  Here we examine the quantitative effect of human BM-MSCs on epidermal regeneration in vitro.
  
  
  METHODS
  Human keratinocytes and BM-MSCs are cultured at ratios from 0% to 100% on top of a fibroblast-embedded collagen gel in a three-dimensional organotypic co-culture model at an air-liquid interface up to 20 days and analyzed by histochemical and immunochemical staining of filaggrin, involucrin and keratin 10 on days 14 and 20. Human BM-MSCs were tracked with quantum dots in organotypic co-cultures.
  
  
  RESULTS
  It was found that epidermal development is strongly influenced by the percentage of co-cultured BM-MSCs. A strong chemotactic effect between keratinocytes and MSCs was seen in the group with 50% of BM-MSCs, which resulted in an impaired epidermal development, whereas at a low percentage of BM-MSCs (10%), a stratified epidermal structure resembling native skin was established on day 14 of culture. Moreover, the immunostaining studies revealed that BM-MSCs in the low percentage (10%) participated in the basal periphery of reconstructed epidermis and a similar pattern characteristic of native epidermis was demonstrated in this experimental group, which was superior to all other experimental groups in terms of the thickness of stratum corneum and the expression profile of epidermal differentiation markers.
  
  
  CONCLUSIONS
  This study indicates the advantage of using a new skin equivalent model incorporating a small fraction of MSCs to develop biologically useful tissues for maintaining homeostasis during skin regeneration and wound healing process.
authors: Department of Medical Technology and Technomathematics, University of Applied Sciences, Aachen, Germany. fillip.laco@strath.ac.uk
authors: Filip
authors: F
authors: Laco
authors: Ma
authors: M
authors: Kun
authors: Hans Joachim
authors: HJ
authors: Weber
authors: S
authors: S
authors: Ramakrishna
authors: Casey K
authors: CK
authors: Chan
mesh_terms: D001854:Bone Marrow Cells / Q000166:cytology / Q000502:physiology*; D018920:Coculture Techniques; D000078404:Epidermal Cells; D004817:Epidermis / Q000502:physiology*; D000091344:Filaggrin Proteins; D006706:Homeostasis / Q000502:physiology; D006801:Humans; D007381:Intermediate Filament Proteins / Q000378:metabolism; D053550:Keratin-10 / Q000378:metabolism; D015603:Keratinocytes / Q000166:cytology / Q000502:physiology*; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000502:physiology*; D008954:Models, Biological; D009924:Organ Culture Techniques; D011498:Protein Precursors / Q000378:metabolism; D045663:Quantum Dots; D012038:Regeneration
chemical_list: C000717249:FLG protein, human; D000091344:Filaggrin Proteins; D007381:Intermediate Filament Proteins; C507493:KRT10 protein, human; D011498:Protein Precursors; D053550:Keratin-10; C032253:involucrin