title: Use of random amplified polymorphic DNA to identify several novel markers for sex identification in the crested serpent eagle and crested goshawk.
abstract: The crested serpent eagle (Spilornis cheela hoya) has no distinct sexual dimorphic traits. In the current study, we report the results of an EE0.6 (EcoRI 0.6-kb fragment) sequence applied to S. cheela hoya and a novel random amplified polymorphic DNA (RAPD) marker that can be used to sex individuals within the species S. cheela hoya and Accipiter trivigatus formosae (crested goshawk). We used sex-specific primers for the avian CHD1 (chromo-helicase-DNA-binding 1) gene and the EE0.6 sequence in PCR assays to determine sex. In addition, 120 random primers were used for RAPD fingerprinting to search for novel sex-specific fragments of S. cheela hoya. The OPBB08 random primer generated a 1241-bp sex-specific fragment in all female S. cheela hoya. From the nucleotide sequence, PCR primers were designed to amplify 553-, 895-, and 194-bp sex-specific fragments present in all female S. cheela hoya. One of these primer pairs (ScBB08-7F/R) also amplified a male/female common fragment that can be used as an internal control (543bp). Moreover, one of the primer pairs (ScBB08-5aF/5bR) could be used to identify genders of A. trivigatus formosae. In conclusion, we identified novel sex-specific DNA markers of S. cheela hoya and A. trivigatus formosae that can be used for rapid and accurate sex identification.
authors: Department of Animal Science and Technology, National Taiwan University, Taipei, Taiwan.
authors: H A
authors: HA
authors: Hsu
authors: P H
authors: PH
authors: Wang
authors: M C
authors: MC
authors: Chao
authors: F T
authors: FT
authors: Chan
authors: L M
authors: LM
authors: Wang
authors: P I
authors: PI
authors: Lin
authors: C H
authors: CH
authors: Chang
authors: H W
authors: HW
authors: Yuan
authors: S T
authors: ST
authors: Ding
mesh_terms: D000818:Animals; D001483:Base Sequence; D016172:DNA Fingerprinting; D004268:DNA-Binding Proteins / Q000235:genetics; D020307:Eagles / Q000033:anatomy & histology / Q000235:genetics*; D005260:Female; D005819:Genetic Markers; D046428:Hawks / Q000033:anatomy & histology / Q000235:genetics*; D008297:Male; D008969:Molecular Sequence Data; D019105:Random Amplified Polymorphic DNA Technique; D016415:Sequence Alignment; D012727:Sex Characteristics; D012732:Sex Determination Analysis / Q000379:methods*
chemical_list: D004268:DNA-Binding Proteins; D005819:Genetic Markers