Effect of RhoA signaling transduction on expression of Ezrin in breast cancer cell lines.
OBJECTIVE
Both RhoA and Ezrin are confirmed to play an important role in the development and metastasis of tumors. However, the mechanism remains unclear. This study rudimentally investigates the regulatory effect of RhoA on the expression of Ezrin.


METHODS
After MDA-MB-231 cells were treated with epidermal growth factor (EGF) or following pretreatment of fasudil (the special inhibitor of RhoA), the expression of RhoA, p-RhoA and Ezrin in MDA-MB-231 cells was detected by western blot.


RESULTS
Stimulation of EGF triggered RhoA phosphorylation in MDA-MB-231 cells which reached the maximum at 30 min; RhoA expression did not change; Ezrin expression was enhanced and reached the maximum at 24 h. However, pretreatment of fasudil before EGF stimulation decreased RhoA phosphorylation and Ezrin expression in MDA-MB-231 cells by 72.73% and 51.28%, respectively.


CONCLUSIONS
RhoA may regulate the invasion and metastasis of breast cancer cells as an upstream signaling of Ezrin.
Ma
Li
L
Department of Pathology, The Fourth Affiliated Hospital, Hebei Medical University, Shijiazhuang, Hebei, PR China.
Liu
Yue-Ping
YP
Zhang
Xiang-Hong
XH
Xing
Ling-Xiao
LX
Wang
Jun-Ling
JL
Geng
Cui-Zhi
CZ
D019307:1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine / Q000031:analogs & derivatives / Q000494:pharmacology; D015153:Blotting, Western; D001943:Breast Neoplasms / Q000378:metabolism / Q000473:pathology; D045744:Cell Line, Tumor; D003598:Cytoskeletal Proteins / Q000096:biosynthesis*; D004815:Epidermal Growth Factor / Q000494:pharmacology; D006801:Humans; D010766:Phosphorylation / Q000187:drug effects; D015398:Signal Transduction / Q000187:drug effects / Q000502:physiology*; D013997:Time Factors; D020742:rhoA GTP-Binding Protein / Q000037:antagonists & inhibitors / Q000378:metabolism*; D000099149:Ezrin
D019307:1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine; D003598:Cytoskeletal Proteins; D004815:Epidermal Growth Factor; D020742:rhoA GTP-Binding Protein; D000099149:Ezrin; C049347:fasudil