Evaluation of chromium(VI) removal behaviour by two isolates of Synechocystis sp. in terms of exopolysaccharide (EPS) production and monomer composition.
Chromium(VI) removal and its association with exopolysaccharide (EPS) production in cyanobacteria were investigated. Synechocystis sp. BASO670 produced higher EPS (548 mg L(-1)) than Synechocystis sp. BASO672 (356 mg L(-1)). While the EC(50) of the Cr(VI) for Synechocystis sp. BASO670 and Synechocystis sp. BASO672 were determined as 11.5 mg L(-1), and 2.0 mg L(-1), respectively, there was no relation between Cr(VI) removal and EPS production. Synechocystis sp. BASO672, which has higher EPS value, removed (33%) more Cr(VI) than Synechocystis sp. BASO670. Monomer compositions of EPS of each of the isolates were determined differently. Synechocystis sp. BASO672 which removed higher Cr(VI), had higher values of uronic acid and glucuronic acid (192 microg/mg and 89%, respectively). Our results showed that EPS might play a role in Cr(VI) tolerance. Monomer composition, especially uronic acid and glucuronic acid content of EPS may have enhanced Cr(VI) removal.
Ozturk
Sahlan
S
Nevsehir University, Faculty of Science and Arts, Department of Biology, 50300 Nevsehir, Turkey. sahlan.ozturk@nevsehir.edu.tr
Aslim
Belma
B
Suludere
Zekiye
Z
D001709:Biotechnology / Q000379:methods*; D002851:Chromatography, High Pressure Liquid; D002857:Chromium / Q000032:analysis* / Q000737:chemistry; D000458:Cyanobacteria / Q000378:metabolism; D020723:Glucuronic Acid / Q000737:chemistry; D019216:Metals, Heavy; D008829:Microbiology; D008855:Microscopy, Electron, Scanning / Q000379:methods; D011134:Polysaccharides / Q000737:chemistry*; D012336:RNA, Ribosomal, 16S / Q000737:chemistry; D013052:Spectrometry, X-Ray Emission / Q000379:methods; D046939:Synechocystis / Q000378:metabolism*; D014574:Uronic Acids / Q000737:chemistry
D019216:Metals, Heavy; D011134:Polysaccharides; D012336:RNA, Ribosomal, 16S; D014574:Uronic Acids; D002857:Chromium; D020723:Glucuronic Acid