The interplay of lipopolysaccharide-binding protein and cytokines in periodontal health and disease.
OBJECTIVE
Periodontal pathogenesis is characterized by Gram-negative bacteria activation of series of pro- and anti-inflammatory cytokines from host cells through the pathway of lipopolysaccharide (LPS), LPS-binding protein (LBP) and CD14. The present study investigated the expression profiles of interleukin (IL)-1beta and IL-10 in periodontal health and disease, and examined the effects of Escherichia coli LPS and LBP interaction on the expression of IL-1beta and IL-10 by human gingival fibroblasts (HGF).


METHODS
Gingival biopsies were collected from 44 subjects with chronic periodontitis and 15 periodontally healthy subjects. The expression of IL-1beta and IL-10 was detected by immunohistochemistry. The mRNA expression of IL-1beta and IL-10 in HGF was detected by RT-PCR with or without recombinant human LBP (rhLBP), while the peptides were analysed by an enzyme-linked immunosorbent assay.


RESULTS
IL-1beta was detected in both oral sulcular epithelia of healthy controls and periodontal pocket epithelia of patients. IL-10 was mainly expressed in the intercellular spaces of connective tissues. IL-1beta displayed a reverse pattern of expression levels with reference to IL-10, and a negative correlation existed between LBP and the ratio of IL-1beta/IL-10. rhLBP suppressed E. coli LPS-induced IL-1beta expression by HGF.


CONCLUSIONS
An appropriate interplay of LBP and cytokines may have a beneficial effect on innate host defence, thereby contributing to periodontal homeostasis.
Ren
Lei
L
Department of Periodontology, Institute of Stomatological Research, Guanghua School of Stomatology, Sun Yat-Sen University, Guangzhou, China.
Jiang
Zi Qiang
ZQ
Fu
Yun
Y
Leung
Wai Keung
WK
Jin
Lijian
L
D000209:Acute-Phase Proteins / Q000032:analysis / Q000276:immunology*; D000293:Adolescent; D000328:Adult; D000368:Aged; D016301:Alveolar Bone Loss / Q000276:immunology / Q000473:pathology; D002352:Carrier Proteins / Q000032:analysis / Q000276:immunology*; D002478:Cells, Cultured; D055113:Chronic Periodontitis / Q000276:immunology / Q000473:pathology; D003238:Connective Tissue / Q000276:immunology / Q000473:pathology; D004846:Epithelial Attachment / Q000276:immunology / Q000473:pathology; D004848:Epithelium / Q000276:immunology / Q000473:pathology; D004926:Escherichia coli / Q000276:immunology; D005110:Extracellular Space / Q000276:immunology; D005347:Fibroblasts / Q000276:immunology; D005881:Gingiva / Q000166:cytology / Q000276:immunology; D006801:Humans; D007113:Immunity, Innate / Q000276:immunology; D016753:Interleukin-10 / Q000032:analysis / Q000276:immunology*; D053583:Interleukin-1beta / Q000032:analysis / Q000276:immunology*; D008070:Lipopolysaccharides / Q000276:immunology*; D008264:Macrophages / Q000276:immunology / Q000473:pathology; D008562:Membrane Glycoproteins / Q000032:analysis / Q000276:immunology*; D008875:Middle Aged; D017622:Periodontal Attachment Loss / Q000276:immunology / Q000473:pathology; D010510:Periodontal Diseases / Q000276:immunology* / Q000473:pathology; D010514:Periodontal Pocket / Q000276:immunology / Q000473:pathology; D010519:Periodontium / Q000276:immunology*; D011994:Recombinant Proteins; D055815:Young Adult; D000099231:Lipopolysaccharide-Binding Protein
D000209:Acute-Phase Proteins; D002352:Carrier Proteins; D016753:Interleukin-10; D053583:Interleukin-1beta; D008070:Lipopolysaccharides; D008562:Membrane Glycoproteins; D011994:Recombinant Proteins; D000099231:Lipopolysaccharide-Binding Protein