Plasmid-based system for high-level gene expression and antisense gene knockdown in Bartonella henselae.
Six broad-host-range plasmid vectors were developed to study gene expression in Bartonella henselae. The vectors were used to express a beta-galactosidase reporter gene in B. henselae and to generate antisense RNA for gene knockdown. When applied to ompR, a putative transcription response regulator of B. henselae, this antisense RNA gene knockdown strategy reduced bacterial invasion of human endothelial cells by over 60%.
Gillaspie
Devin
D
Department of Molecular Medicine, College of Medicine, MDC7, University of South Florida, Tampa, FL 33612, USA.
Perkins
Izabella
I
Larsen
Kellie
K
McCord
Amy
A
Pangonis
Scott
S
Sweger
Debra
D
Seleem
Mohamed N
MN
Sriranganathan
Nammalwar
N
Anderson
Burt E
BE
D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D018416:Bartonella henselae / Q000235:genetics / Q000378:metabolism / Q000472:pathogenicity*; D042783:Endothelial Cells / Q000382:microbiology*; D015964:Gene Expression Regulation, Bacterial; D055785:Gene Knockdown Techniques; D017930:Genes, Reporter; D005822:Genetic Vectors; D006801:Humans; D010957:Plasmids / Q000235:genetics*; D016372:RNA, Antisense; D001616:beta-Galactosidase / Q000235:genetics / Q000378:metabolism*
D001426:Bacterial Proteins; D016372:RNA, Antisense; D001616:beta-Galactosidase