Solid-liquid interface method (SLIM): a new crystallization method for proteins.
Despite impressive advances in theories, methods and technologies, crystallization still remains a serious bottleneck in structural determination of macromolecules. Here we present a novel solid-liquid interface method (SLIM) for protein crystallization, based on the pre-adding and drying of a crystallization reagent, and thereafter the dispensing of a protein solution to the dried media to initiate crystallization from the solid-liquid interface. Not only quick and easy to perform, the method also allows for a less concentrated protein solution for setting up crystallization trials.
Brostromer
Erik
E
National Laboratory of Protein Engineering and Plant Genetic Engineering, College of Life Sciences, Peking University, Beijing 100871, People's Republic of China.
Nan
Jie
J
Li
Lan-Fen
LF
Su
Xiao-Dong
XD
D003460:Crystallization / Q000379:methods*; D011506:Proteins / Q000737:chemistry*
D011506:Proteins