title: MCC, a new interacting protein for Scrib, is required for cell migration in epithelial cells.
abstract: To further characterize the molecular events supporting the tumor suppressor activity of Scrib in mammals, we aim to identify new binding partners. We isolated MCC, a recently identified binding partner for beta-catenin, as a new interacting protein for Scrib. MCC interacts with both Scrib and the NHERF1/NHERF2/Ezrin complex in a PDZ-dependent manner. In T47D cells, MCC and Scrib proteins colocalize at the cell membrane and reduced expression of MCC results in impaired cell migration. By contrast to Scrib, MCC inhibits cell directed migration independently of Rac1, Cdc42 and PAK activation. Altogether, these results identify MCC as a potential scaffold protein regulating cell movement and able to bind Scrib, beta-catenin and NHERF1/2.
authors: INSERM UMR891, Centre de Recherche en Cancérologie de Marseille, Pharmacologie Moléculaire, Marseille F-13009, France.
authors: Camille
authors: C
authors: Arnaud
authors: Michaël
authors: M
authors: Sebbagh
authors: Sébastien
authors: S
authors: Nola
authors: Stéphane
authors: S
authors: Audebert
authors: Ghislain
authors: G
authors: Bidaut
authors: Aurélie
authors: A
authors: Hermant
authors: Odile
authors: O
authors: Gayet
authors: Nelson J
authors: NJ
authors: Dusetti
authors: Vincent
authors: V
authors: Ollendorff
authors: Marie-Josée
authors: MJ
authors: Santoni
authors: Jean-Paul
authors: JP
authors: Borg
authors: Patrick
authors: P
authors: Lécine
mesh_terms: D000818:Animals; D002460:Cell Line; D002465:Cell Movement / Q000502:physiology*; D004789:Enzyme Activation; D004847:Epithelial Cells / Q000166:cytology / Q000502:physiology*; D006801:Humans; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D010750:Phosphoproteins / Q000235:genetics / Q000378:metabolism; D011485:Protein Binding; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D017923:Sodium-Hydrogen Exchangers / Q000235:genetics / Q000378:metabolism; D025521:Tumor Suppressor Proteins / Q000235:genetics / Q000378:metabolism*; D051176:beta Catenin / Q000378:metabolism; D020764:cdc42 GTP-Binding Protein / Q000378:metabolism; D020830:rac1 GTP-Binding Protein / Q000378:metabolism
chemical_list: D008565:Membrane Proteins; D010750:Phosphoproteins; D011993:Recombinant Fusion Proteins; C480591:SCRIB protein, human; D017923:Sodium-Hydrogen Exchangers; D025521:Tumor Suppressor Proteins; D051176:beta Catenin; C093376:sodium-hydrogen exchanger regulatory factor; C067857:MCC protein, human; D020764:cdc42 GTP-Binding Protein; D020830:rac1 GTP-Binding Protein