title: Preparation and properties of dextran sulfate-lysozyme conjugate.
abstract: The purpose of this research was to conjugate lysozyme with dextran sulfate (DS) under mild Maillard reaction conditions and to investigate the functional properties of the resulting conjugate. The covalent attachment of DS to lysozyme was confirmed by SDS-PAGE and cation-exchange chromatography. Lytic activity of the conjugated and uncongugated lysozyme toward Micrococcus lysodeikticus was 40 and 71% that of native lysozyme, respectively. The lysozyme-DS conjugate was highly soluble at alkaline pH values and different temperatures (25, 40, and 60 degrees C). Moreover, the modified lysozyme exhibited increased heat stability, with better emulsion activity and emulsion stability than those of unmodified proteins. Lysozyme-DS conjugate showed antibacterial activity and significantly inhibited growth of Staphylococcus aureus and Escherichia coli in a dose-dependent manner. Taken together, the result of this study demonstrated that conjugation might increase the applicability of lysozyme in different food products for improving functional properties.
authors: Department of Food Science and Technology, School of Agriculture.
authors: Zahra
authors: Z
authors: Alahdad
authors: Roqhayeh
authors: R
authors: Ramezani
authors: Mahmoud
authors: M
authors: Aminlari
authors: Mahsa
authors: M
authors: Majzoobi
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D002852:Chromatography, Ion Exchange; D016264:Dextran Sulfate / Q000737:chemistry*; D004591:Electrophoresis, Polyacrylamide Gel; D045703:Emulsifying Agents; D004795:Enzyme Stability; D004926:Escherichia coli / Q000187:drug effects; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D015416:Maillard Reaction; D008837:Micrococcus / Q000378:metabolism; D009113:Muramidase / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology*; D012995:Solubility; D013211:Staphylococcus aureus / Q000187:drug effects; D013329:Structure-Activity Relationship
chemical_list: D000900:Anti-Bacterial Agents; D045703:Emulsifying Agents; D016264:Dextran Sulfate; D009113:Muramidase