title: The expression of the Goodpasture antigen-binding protein (ceramide transporter) in adult rat brain.
abstract: The Goodpasture antigen-binding protein (GPBP) plays a critical role in brain development. Knockdown of GPBP leads to loss of myelinated tracts in the central nervous system and to extensive apoptosis in the brain during early embryogenesis. GPBP was initially identified as a protein associated with the autoantigen in Goodpasture autoimmune syndrome, where it was shown to be a kinase that regulates type IV collagen organization. GPBP isoforms bind and transport ceramide from the endoplasmic reticulum to the Golgi apparatus and are therefore also known as ceramide transporters (CERT). Ceramide dysregulation is involved in autoimmunity and neurodegenerative disorders. In order to analyze the possible role of GPBP in neuroinflammation and neurodegeneration we studied the basal GPBP expression in normal rat brain. High levels of immunoreactivity were detected in neurons of the cerebral cortex, hippocampal formation, the basal ganglia, the olfactory bulb and nuclei of the thalamus, the hypothalamus and the septal area. Lower expression levels of GPBP were observed widely throughout the brain, suggesting that GPBP plays an important role in central nervous system neuron function.
authors: Department of Neuroscience, School of Mental Health and Neuroscience, Maastricht University, Maastricht, The Netherlands.
authors: Chiara
authors: C
authors: Mencarelli
authors: Caroline
authors: C
authors: Hammels
authors: Joost
authors: J
authors: Van Den Broeck
authors: Mario
authors: M
authors: Losen
authors: Hellen
authors: H
authors: Steinbusch
authors: Francisco
authors: F
authors: Revert
authors: Juan
authors: J
authors: Saus
authors: David A
authors: DA
authors: Hopkins
authors: Marc H
authors: MH
authors: De Baets
authors: Harry W
authors: HW
authors: Steinbusch
authors: Pilar
authors: P
authors: Martinez-Martinez
mesh_terms: D000818:Animals; D015153:Blotting, Western; D001921:Brain / Q000033:anatomy & histology / Q000201:enzymology*; D001931:Brain Mapping; D002518:Ceramides / Q000378:metabolism*; D004027:Diencephalon / Q000033:anatomy & histology / Q000201:enzymology; D004721:Endoplasmic Reticulum / Q000378:metabolism; D006056:Golgi Apparatus / Q000378:metabolism; D007150:Immunohistochemistry; D008297:Male; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D013687:Telencephalon / Q000033:anatomy & histology / Q000201:enzymology
chemical_list: D002518:Ceramides; C545703:Cert1 protein, rat; D017346:Protein Serine-Threonine Kinases