Short- and long-term effects of maternal nicotine exposure during lactation on body adiposity, lipid profile, and thyroid function of rat offspring.
Epidemiological studies show a higher prevalence of obesity in children from smoking mothers and smoking may affect human thyroid function. To evaluate the mechanism of smoking as an imprinting factor for these dysfunctions, we evaluated the programming effects of maternal nicotine (NIC) exposure during lactation. Two days after birth, osmotic minipumps were implanted in lactating rats, divided into: NIC (6 mg/kg per day s.c.) for 14 days; Control - saline. All the significant data were P<0.05 or less. Body weight was increased from 165 days old onwards in NIC offspring. Both during exposure (at 15 days old) and in adulthood (180 days old), NIC group showed higher total fat (27 and 33%). In addition, NIC offspring presented increased visceral fat and total body protein. Lipid profile was not changed in adulthood. Leptinemia was higher at 15 and 180 days old (36 and 113%), with no changes in food intake. Concerning the thyroid status, the 15-days-old NIC offspring showed lower serum-free tri-iodothyronine (FT(3)) and thyroxine (FT(4)) with higher TSH. The 180-days-old NIC offspring exhibited lower TSH, FT(3), and FT(4)). In both periods, liver type 1 deiodinase was lower (26 and 55%). We evidenced that NIC imprints a neonatal thyroid dysfunction and programs for a higher adiposity, hyperleptinemia, and secondary hypothyroidism in adulthood. Our study identifies lactation as a critical period to NIC programming for obesity, with hypothyroidism being a possible contributing factor.
Oliveira
E
E
Department of Physiological Sciences, Roberto Alcantara Gomes Biology Institute, State University of Rio de Janeiro, Rio de Janeiro 20551-030, Brazil.
Moura
E G
EG
Santos-Silva
A P
AP
Fagundes
A T S
AT
Rios
A S
AS
Abreu-Villaça
Y
Y
Nogueira Neto
J F
JF
Passos
M C F
MC
Lisboa
P C
PC
D000273:Adipose Tissue / Q000187:drug effects* / Q000254:growth & development; D000818:Animals; D000833:Animals, Suckling; D001835:Body Weight / Q000187:drug effects / Q000502:physiology; D050171:Dyslipidemias / Q000378:metabolism*; D005260:Female; D015918:Infusion Pumps, Implantable; D007774:Lactation; D020738:Leptin / Q000097:blood; D008055:Lipids / Q000097:blood; D008297:Male; D009538:Nicotine / Q000494:pharmacology*; D018722:Nicotinic Agonists / Q000494:pharmacology*; D011247:Pregnancy; D051381:Rats; D017208:Rats, Wistar; D013961:Thyroid Gland / Q000254:growth & development / Q000502:physiology*; D013972:Thyrotropin / Q000097:blood; D013974:Thyroxine / Q000097:blood; D014284:Triiodothyronine / Q000097:blood
D020738:Leptin; D008055:Lipids; D018722:Nicotinic Agonists; D014284:Triiodothyronine; D009538:Nicotine; D013972:Thyrotropin; D013974:Thyroxine