title: Solid-liquid interface method (SLIM): a new crystallization method for proteins.
abstract: Despite impressive advances in theories, methods and technologies, crystallization still remains a serious bottleneck in structural determination of macromolecules. Here we present a novel solid-liquid interface method (SLIM) for protein crystallization, based on the pre-adding and drying of a crystallization reagent, and thereafter the dispensing of a protein solution to the dried media to initiate crystallization from the solid-liquid interface. Not only quick and easy to perform, the method also allows for a less concentrated protein solution for setting up crystallization trials.
authors: National Laboratory of Protein Engineering and Plant Genetic Engineering, College of Life Sciences, Peking University, Beijing 100871, People's Republic of China.
authors: Erik
authors: E
authors: Brostromer
authors: Jie
authors: J
authors: Nan
authors: Lan-Fen
authors: LF
authors: Li
authors: Xiao-Dong
authors: XD
authors: Su
mesh_terms: D003460:Crystallization / Q000379:methods*; D011506:Proteins / Q000737:chemistry*
chemical_list: D011506:Proteins