Investigation of blood typing method for seoul National University miniature pig.
Pig blood group antigens may be present on grafted tissue as 16 isoforms, including the major one, A substrance. Seoul National University (SNU) miniature pigs and domestic pigs were used in this study. Polymerase chain reaction (PCR) was performed for the erythrocyte antigen A (EAA) gene, and reverse transcriptase-PCR for pig A transferase fucosyl transferase (FUT) 1 and FUT-2. The hemagglutination test was performed with murine monoclonal anti-A and anti-B antibodies (mAb), and immunohistochemistry with anti-human blood antigen mAb. SNU miniature and domestic pigs showed blood groups A and O. Blood group A SNU miniature pigs expressed either EAA(AA) or EAA(AO) and either S(SS) or S(SO); blood group O miniature pigs expressed EAA(OO) and S(SS) or S(SO), and there was no A(weak). Additionally, blood group A could be divided into blood group A(clotting) and blood group A(not clotting) in hemagglutination tests. Pig A substance was expressed in the lung and kidney in blood group A pigs, but we could not detect pig A substance expression in the lung, kidney, and heart of blood group O pigs or the heart of blood group A pigs. In conclusion, we suggest that blood typing of SNU miniature pigs can be easily performed using immunohistochemistry, PCR, and/or RT-PCR. Molecular-based AO typing described in this study may be useful to select SNU miniature pigs bearing a specific blood group.
Yeom
S-C
SC
Center for Animal Resource and Development, Bio-max Institute, Seoul National University, Seoul, Republic of Korea.
Oh
B-C
BC
Cho
S-Y
SY
Park
C-G
CG
Lee
B-C
BC
Lee
W-J
WJ
D000818:Animals; D000911:Antibodies, Monoclonal; D001788:Blood Grouping and Crossmatching / Q000379:methods*; D001803:Blood Transfusion / Q000379:methods; D020077:Endogenous Retroviruses / Q000472:pathogenicity; D006801:Humans; D007723:Korea; D016133:Polymerase Chain Reaction; D012194:RNA-Directed DNA Polymerase; D013552:Swine / Q000097:blood; D013556:Swine, Miniature / Q000097:blood*; D014183:Transplantation, Heterologous; D015047:Zoonoses / Q000635:transmission
D000911:Antibodies, Monoclonal; D012194:RNA-Directed DNA Polymerase