Dendritic chain reaction.
Signal amplification techniques are broadly used to improve the detection sensitivity of various analytes for diagnostic purposes. We have developed a novel, non-PCR-based modular technique for exponential amplification of diagnostic signals that is conveniently performed in an aqueous environment. The technique is based on a distinctive dendritic chain reaction (DCR); the diagnostic signal is generated upon disassembly of a self-immolative dendrimer that releases chromogenic molecules. Under ideal conditions, a single analyte molecule initiates a DCR that generates a strong diagnostic signal. When coupled with a protease diagnostic probe, the DCR technique detected the activity of penicillin-G-amidase with high sensitivity. This is the first example of exponential signal amplification performed under aqueous conditions that is not based on PCR.
Sella
Eran
E
School of Chemistry, Raymond and Beverly Sackler Faculty of Exact Sciences, Tel-Aviv University, Tel Aviv 69978, Israel.
Shabat
Doron
D
D000581:Amidohydrolases / Q000032:analysis / Q000378:metabolism; D002623:Chemistry Techniques, Analytical / Q000379:methods*; D050091:Dendrimers / Q000032:analysis* / Q000138:chemical synthesis* / Q000737:chemistry; D003955:Diagnostic Tests, Routine / Q000379:methods; D006861:Hydrogen Peroxide / Q000737:chemistry; D015335:Molecular Probes / Q000032:analysis / Q000737:chemistry; D010400:Penicillin G / Q000032:analysis / Q000378:metabolism; D012680:Sensitivity and Specificity
D050091:Dendrimers; D015335:Molecular Probes; D006861:Hydrogen Peroxide; D000581:Amidohydrolases; C019665:amidase; D010400:Penicillin G