Increase in serum haptoglobin and apolipoprotein M in patients with obstructive sleep apnoea.
Obstructive sleep apnoea (OSA) is a common syndrome, which is associated with a number of medical problems that impact morbidity and mortality. Although the precise mechanisms that underlie these associations are not fully understood, previous studies have suggested that patients with OSA show elevations of several biomarkers that are associated with cardiovascular risk. This study was undertaken to identify serum proteins associated with OSA by using a proteomics technique and to examine changes in identified protein levels after continuous positive airway pressure treatment. The study participants consisted of 40 male patients (aged 40-49 years) with severe OSA and 34 male control subjects matched for age and body mass index. All subjects underwent polysomnography. Using a proteomics approach, we identified nine proteins that were differentially expressed in patients with severe OSA and controls. Three of these nine proteins, haptoglobin, paraoxonase-1 and apolipoprotein M, were quantified by using enzyme-linked immunosorbent assays, kinetic assays and by using Western blotting. Multiple regression analysis showed that haptoglobin and apolipoprotein M levels are independently related to apnoea-hypopnoea index (P < 0.01). A further study is required to determine the nature of associations between these identified proteins and OSA in a large population.
Kim
Jinkwan
J
Pulmonary Sleep Disorder Center, Ansan Hospital, Korea University Medical Center, Ansan, Gyeonggi-do 425-707, South Korea.
Lee
Seunggwan
S
In
Kwangho
K
Kim
Jehyeong
J
You
Sehwa
S
Kang
Kyungho
K
Sim
Jaejeong
J
Lee
Sangyeub
S
Yoon
Daewui
D
Lee
Jungbok
J
Shin
Chol
C
D000328:Adult; D001053:Apolipoproteins / Q000097:blood*; D000075243:Apolipoproteins M; D043303:Aryldialkylphosphatase; D015415:Biomarkers / Q000097:blood; D002318:Cardiovascular Diseases / Q000097:blood / Q000175:diagnosis; D045422:Continuous Positive Airway Pressure; D015180:Electrophoresis, Gel, Two-Dimensional; D006242:Haptoglobins / Q000378:metabolism*; D006801:Humans; D054834:Lipocalins; D008297:Male; D008875:Middle Aged; D017286:Polysomnography; D040901:Proteomics; D012307:Risk Factors; D020181:Sleep Apnea, Obstructive / Q000097:blood* / Q000175:diagnosis / Q000628:therapy; D016896:Treatment Outcome
C400523:APOM protein, human; D001053:Apolipoproteins; D000075243:Apolipoproteins M; D015415:Biomarkers; C485359:HP protein, human; D006242:Haptoglobins; D054834:Lipocalins; D043303:Aryldialkylphosphatase; C468406:PON1 protein, human