Separation of triacylglycerols and free fatty acids in microalgal lipids by solid-phase extraction for separate fatty acid profiling analysis by gas chromatography.
Microalgal lipids were separated into two fractions, triacylglycerols (TAGs) and free fatty acids (FFAs), by solid-phase extraction employing sodium carbonate as the sorbent and dichloromethane (20% by volume) in n-hexane as the extracting solvent. The TAG fraction was then saponified, followed by acidification, extraction and tert-butyldimethylsilyl esterification. The FFA fraction was directly acidified, extracted and derivatized. From the lipid extracts of eight microalgal species examined, a total of 13 fatty acids were detected in the TAG fractions and nine were found in the FFA fractions, with at much higher total TAG content in all microalgae. Oleic acid was the most prominent fatty acid in three species, alpha-linolenic acid was more abundant in two others, and palmitic acid was present in highest concentration in the remaining three species.
Paik
Man-Jeong
MJ
Metabolomic Analysis Laboratory, Institute for Neuroregeneration and Stem Cell Research, and Department of Molecular Science and Technology, School of Medicine, Ajou University, Suwon 443-721, South Korea.
Kim
Hoon
H
Lee
Jinwoo
J
Brand
Jerry
J
Kim
Kyoung-Rae
KR
D002254:Carbonates / Q000737:chemistry; D002849:Chromatography, Gas / Q000379:methods*; D056890:Eukaryota / Q000737:chemistry*; D005230:Fatty Acids, Nonesterified / Q000032:analysis*; D013058:Mass Spectrometry / Q000379:methods; D017646:Organosilicon Compounds; D012044:Regression Analysis; D015203:Reproducibility of Results; D052616:Solid Phase Extraction / Q000379:methods*; D014280:Triglycerides / Q000032:analysis*
D002254:Carbonates; D005230:Fatty Acids, Nonesterified; D017646:Organosilicon Compounds; D014280:Triglycerides; C009660:t-butyldimethylsilyl compounds; C005686:sodium carbonate