Connexin43 interacts with Caveolin-3 in the heart.
Gap junctions (GJs), collections of multiple intercellular channels between neighboring cells, are specialized channels facilitating intercellular electrical and chemical communication. GJs are important for synchronizing coupling and coordinated contraction in the heart, and are crucial regulators of heart gene transcription, cardiac development, and protection of ischemic cardiomyocytes through second messenger communication. Identification of proteins that interact with Connexin43 (Cx43), the predominant protein in cardiac GJs, may contribute to the understanding of GJ functional regulation. Using a yeast two-hybrid system, we identified Caveolin-3 (Cav3) as a new Cx43-interacting protein. This interaction was confirmed by co-immunoprecipitation and co-localization experiments. CX43 interacts with Cav3, suggesting that Cav3 may participate in the functional regulation of GJs.
Liu
Limei
L
Department of Cardiology, The First Affiliated Hospital, Shantou University Medical College, 57 Changping Road, 515041, Shantou, People's Republic of China.
Li
Yuguang
Y
Lin
Jijin
J
Liang
Qing
Q
Sheng
Xiuzhang
X
Wu
Jun
J
Huang
Ruiyan
R
Liu
Shukai
S
Li
Yanyan
Y
D001483:Base Sequence; D051244:Caveolin 3 / Q000378:metabolism*; D018031:Connexin 43 / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D049452:Green Fluorescent Proteins / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D047468:Immunoprecipitation; D008969:Molecular Sequence Data; D009206:Myocardium / Q000378:metabolism*; D010957:Plasmids / Q000235:genetics; D011485:Protein Binding; D021381:Protein Transport; D020798:Two-Hybrid System Techniques
D051244:Caveolin 3; D018031:Connexin 43; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins