Ex vivo transfer of nuclear factor-kappaB decoy ameliorates hepatic cold ischemia/reperfusion injury.
Cold ischemia/reperfusion injury of the hepatic graft has been attributed to the release of various inflammatory cytokines. Specific inhibition of these cytokines may improve viability of the hepatic graft upon reperfusion. Herein we have assessed the efficacy of cis element decoy against nuclear factor-kappaB binding site delivery to the hepatic tissue in a rodent liver transplantation model. At 8 hours after reperfusion of the liver, significant reduction was noted in the livers treated with decoy in the release of cytosolic enzymes from the hepatocytes and in serum tumor necrosis factor alpha (P < .05). The neutrophilic infiltration into the hepatic grafts was significantly suppressed in the livers treated with decoy oligodeoxynucleotides (ODNs). Decoy ODNs against nuclear factor-kappaB binding site delivery improved the viability of the hepatic graft against cold ischemia/reperfusion injury in the rodent liver transplantation model.
Yoshizumi
T
T
Department of Surgery and Science, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan. yosizumi@surg2.med.kyushu-u.ac.jp
Ikeda
Y
Y
Kaneda
Y
Y
Sueishi
K
K
D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D016207:Cytokines / Q000502:physiology; D007249:Inflammation / Q000517:prevention & control; D016209:Interleukin-8 / Q000097:blood; D007770:L-Lactate Dehydrogenase / Q000097:blood; D008081:Liposomes; D008102:Liver Circulation / Q000502:physiology*; D016031:Liver Transplantation / Q000379:methods* / Q000473:pathology; D008297:Male; D016328:NF-kappa B / Q000187:drug effects / Q000502:physiology*; D009504:Neutrophils / Q000502:physiology; D009838:Oligodeoxyribonucleotides / Q000494:pharmacology*; D051381:Rats; D011914:Rats, Inbred BN; D015427:Reperfusion Injury / Q000517:prevention & control*; D029082:Sendai virus / Q000502:physiology*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
D016207:Cytokines; D016209:Interleukin-8; D008081:Liposomes; D016328:NF-kappa B; D009838:Oligodeoxyribonucleotides; D014409:Tumor Necrosis Factor-alpha; D007770:L-Lactate Dehydrogenase; D000410:Alanine Transaminase