Development of an automatic solid phase extraction and liquid chromatography mass spectrometry method by using a monolithic column for the analysis of Cyclosporin A in human plasma.
A sensitive and specific and automated liquid chromatography-electrospray mass spectrometric (LC-ESI-MS) assay for the quantification of Cyclosporin A in human plasma was developed. Following a simple protein precipitation step, the supernatant was extracted on-line and directly injected into the system LC-ESI-MS. A relatively new type of monolithic column consisting of a silica rod with bimodal pore structure was used to achieve a retention time of 2.4 min with a very low backpressure at a flow rate of 1 ml/min. The assay was linear from 0.050 to 1.000 microg/ml. The mean recovery was 91%. The mean inter-day and intra-day precisions were 1.85% and 2.83%, respectively. The combination of the automated solid phase extraction and the low retention time achieved with this columns increase the throughput and decrease the time of analysis of each sample. This technology is useful in order to improve the efficiency of the bioanalytical studies.
Alvarez
Covadonga
C
Department of Pharmaceutical Technology, Faculty of Pharmacy, Complutense University of Madrid, 28040 Madrid, Spain.
Wainer
Irving W
IW
D001331:Automation; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D016572:Cyclosporine / Q000097:blood*; D006801:Humans; D008722:Methods; D009862:Online Systems; D015203:Reproducibility of Results; D012822:Silicon Dioxide; D052616:Solid Phase Extraction / Q000379:methods*; D053719:Tandem Mass Spectrometry / Q000379:methods* / Q000592:standards
D012822:Silicon Dioxide; D016572:Cyclosporine