Activin A induces ovine follicle stimulating hormone beta using -169/-58 bp of its promoter and a simple TATA box.
BACKGROUND
Activin A increases production of follicle stimulating hormone (FSH) by inducing transcription of its beta subunit (FSHB). This induction has been studied here in LbetaT2 gonadotropes using transient expression of ovine FSHBLuc (-4741 bp of ovine FSHB promoter plus exon/intron 1 linked to Luc). Several sequences between -169/-58 bp of the ovine FSHB proximal promoter are necessary for induction by activin A in LbetaT2 cells, but deletions between -4741/-752 bp decrease induction > 70% suggesting the existence of other important 5' sequences. Induction disappears if a minimal T81 thymidine kinase promoter replaces the ovine FSHB TATA box and 3' exon/intron. The study reported here was designed to determine if sequences outside -169/-58 bp are important for induction of ovine FSHB by activin A.


METHODS
Progressively longer deletions of ovine FSHBLuc were created between -4741/-195 bp. Deletions internal to this region were created also, but replaced with substitute DNA. The ovine FSHB TATA box region (-40/+3 bp) was replaced by thymidine kinase and rat prolactin minimal promoters, and substitutions were made in 3' intron/exon sequences. All constructs were tested for basal and activin A-induced expression in LbetaT2 cells.


RESULTS
Successive 5' deletions progressively lowered fold-induction by activin A from 9.5 to zero, but progressively increased basal expression. Replacing deletions with substitute DNA showed no changes in basal expression or fold-induction. Induction by activin A was supported by the minimal rat prolactin promoter (TATA box) but not the thymidine kinase promoter (no TATA box). Replacement mutations in the 3' region did not decrease induction by activin A.


CONCLUSIONS
The data show that specific ovine FSHB sequences 5' to -175 bp or 3' of the transcription start site are not required for induction by activin A. A minimal TATA box promoter supports induction by activin A, but the sequence between the TATA box and transcription start site seems unimportant.
Han
Sang-oh
SO
Department of Molecular and Structural Biochemistry, North Carolina State University, Raleigh, 27695-7622, USA. shan@ncsu.edu
Miller
William L
WL
D028341:Activins / Q000494:pharmacology*; D000818:Animals; D001483:Base Sequence; D037201:Follicle Stimulating Hormone, beta Subunit / Q000096:biosynthesis / Q000235:genetics*; D052681:Gonadotrophs / Q000378:metabolism; D051379:Mice; D011401:Promoter Regions, Genetic / Q000235:genetics*; D051381:Rats; D012756:Sheep; D016385:TATA Box
D037201:Follicle Stimulating Hormone, beta Subunit; C422405:activin A; D028341:Activins