Synthesis and application of photoaffinity probe containing an intact isoprenoid chain.
Two novel chemical probes each carrying an intact isoprenoid chain, a biotin tag and a benzophenone moiety were synthesized. Photoaffinity labeling of the Saccharomyces cerevisiae cell lysate revealed that these probes could selectively trap some proteins, and proteins with molecular weight of approximately 70 KDa appeared as a major band upon Streptavidin blot analysis.
Li
Lingdong
L
Dalian Institute of Chemical Physics, CAS, Dalian 116023, PR China.
Tang
Wei
W
Zhao
Zongbao Kent
ZK
D019920:Photoaffinity Labels / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology; D011106:Polyisoprenyl Phosphates / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry / Q000378:metabolism; D013729:Terpenes / Q000138:chemical synthesis* / Q000737:chemistry; D014466:Ultraviolet Rays
D019920:Photoaffinity Labels; D011106:Polyisoprenyl Phosphates; D029701:Saccharomyces cerevisiae Proteins; D013729:Terpenes; C011082:polyprenyl monophosphate