[Expression of PI3K/Akt signal pathway in acute myocardial ischemia].
OBJECTIVE
To explore the influence of the PI3K/Akt-mediated signal pathway in acute myocardial ischemia (AMI) rats, the expression of pAkt, Akt, Caspase3 and p38 in myocardium and somatic muscles of AMI rats were detected.


METHODS
The rats model of AMI were established by peritoneal injecting isoprinosine (ISO), and were detected by electrocardiogram and haemodynamics. The expressions of pAkt, Caspase3 and p38 in somatic muscles and cardiac muscles of AMI and normal rats were detected by Dot blot hybridization and Western blot.


RESULTS
In contrast with normal rats, electrocardiogram of AMI rats showed a lower displacement of ST segment (> or = 0.1 mv). The expression of pAkt, Caspase3 and p38 were higher than those in normal rats (P < 0.05). No apparent changes were observed in expression of Akt (P = 0.477).


CONCLUSIONS
Expressions of pAkt, Akt and correlated apoptosis molecule Caspase3 and p38 in cardiac and somatic muscles of AMI rats were higher than those in normal rats. No apparent changes were observed in expression of Akt.
Song
Ruixia
R
Key Laboratory of Environment and Gene Related to Diseases, Medical College of Xi'an Jiaotong University, Ministry of Education, Xi'an 710061, China. song-ruixia@126.com
Xiong
Yongmin
Y
Chen
Qun
Q
Zou
Xiuzhen
X
D000818:Animals; D053148:Caspase 3 / Q000378:metabolism; D005260:Female; D008297:Male; D017202:Myocardial Ischemia / Q000378:metabolism*; D051058:Oncogene Protein v-akt / Q000378:metabolism*; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D015398:Signal Transduction; D048051:p38 Mitogen-Activated Protein Kinases / Q000378:metabolism*
D019869:Phosphatidylinositol 3-Kinases; D051058:Oncogene Protein v-akt; D048051:p38 Mitogen-Activated Protein Kinases; C505410:Casp3 protein, rat; D053148:Caspase 3