Rapid Raman imaging of stable, functionalized nanoshells in mammalian cell cultures.
Two Raman-active poly(ethylene glycol) (PEG) molecules, one linear (MW 5000) and the other branched (MW 2420), are synthesized to stabilize gold-silica nanoshells in cell culture media and track nanoparticles in mammalian cell cultures. The linear PEG provides greater nanoshell stability in saline solution compared to commercially available PEG-thiol or the branched PEG. Surface enhanced Raman scattering rapidly tracks the probes and provides semiquantitative information regarding particle localization within mouse macrophage (RAW 264.7) and human breast cancer (MCF 7) cell cultures.
Huang
Yiming
Y
BCPS Department, Illinois Institute of Technology, 3101 South Dearborn, Chicago, IL, USA.
Swarup
Vimal P
VP
Bishnoi
Sandra Whaley
SW
D000818:Animals; D045744:Cell Line, Tumor; D006046:Gold / Q000737:chemistry*; D006801:Humans; D053768:Metal Nanoparticles / Q000737:chemistry*; D051379:Mice; D015394:Molecular Structure; D011092:Polyethylene Glycols / Q000737:chemistry; D013059:Spectrum Analysis, Raman
D011092:Polyethylene Glycols; D006046:Gold