title: Enhanced degradation of TNT by genome-shuffled Stenotrophomonas maltophilia OK-5.
abstract: In this study, the enhanced degradation of TNT using cultures of genome-shuffled Stenotrophomonas maltophilia OK-5 mt-3 has been examined and the proteome of shuffled strain was compared to the wild-type OK-5 strain. Genome shuffling of S. maltophilia OK-5 was used to achieve a rapid enhancement of TNT degradation. The initial mutant population was generated by NTG treatment and UV irradiation. The wild-type OK-5 strain was able to degrade 0.2 mM TNT within 6 days, yet barely tolerated 0.5 mM TNT while the shuffled OK-5 mt-3 was capable of completely degrading 0.5 mM TNT within 8 days, and 1.2 mM within 24 days. The proteomic analysis of the shuffled OK-5 mt-3 demonstrated the changes in the expression levels of certain proteins compared to wild-type OK-5. These results provide clues for understanding TNT tolerance and improved TNT degradation by shuffled S. maltophilia OK-5 mt-3 and have possible applications in the processing of industrial waste containing relatively high TNT concentrations.
authors: Division of Biological Sciences, Kosin University, Busan, 606-701, Republic of Korea.
authors: Bheong-Uk
authors: BU
authors: Lee
authors: Yun-Seok
authors: YS
authors: Cho
authors: Sung-Chul
authors: SC
authors: Park
authors: Kye-Heon
authors: KH
authors: Oh
mesh_terms: D001426:Bacterial Proteins / Q000032:analysis; D016000:Cluster Analysis; D032621:DNA Shuffling; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics*; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D015180:Electrophoresis, Gel, Two-Dimensional; D016680:Genome, Bacterial; D008969:Molecular Sequence Data; D009153:Mutagens / Q000494:pharmacology; D009604:Nitrosoguanidines / Q000494:pharmacology; D010802:Phylogeny; D020543:Proteome / Q000032:analysis; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D020615:Stenotrophomonas maltophilia / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism*; D014303:Trinitrotoluene / Q000378:metabolism*; D014466:Ultraviolet Rays
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D009153:Mutagens; D009604:Nitrosoguanidines; D020543:Proteome; D012336:RNA, Ribosomal, 16S; D014303:Trinitrotoluene