title: Down-regulated expression of SATB2 is associated with metastasis and poor prognosis in colorectal cancer.
abstract: To identify novel biomarkers of metastasis of colorectal cancer (CRC), we developed an orthotopic implantation model of murine CRC and selected in vivo M5, a subclone of the SW480 CRC cell line with enhanced potential for metastasis to the liver. We compared the differences in the gene expression profiles between M5 and SW480 cells using gene expression profiling. We found that expression of special AT-rich sequence-binding protein 2 (SATB2) was down-regulated in M5 cells. Immunohistochemical analysis of 146 colorectal tumour samples showed that underexpression of SATB2 was strongly correlated with poor prognosis, tumour invasion, lymph node metastasis, distant metastasis, and Dukes' classification for CRC. Univariate and multivariate survival analyses further showed that SATB2 expression was a potential favourable prognostic factor for CRC. These results demonstrated not only that SATB2 is a potential novel prognostic factor for CRC, but also that selection of a highly metastatic clone of SW480 in vivo coupled with gene expression profiling is a powerful approach to identifying prognostic markers for CRC.
authors: Department of Pathology, School of Basic Medical Sciences, Southern Medical University, Guangzhou, People's Republic of China.
authors: Shuang
authors: S
authors: Wang
authors: Jun
authors: J
authors: Zhou
authors: Xiao-Yan
authors: XY
authors: Wang
authors: Jun-Mei
authors: JM
authors: Hao
authors: Juan-Zhi
authors: JZ
authors: Chen
authors: Xiang-Mei
authors: XM
authors: Zhang
authors: He
authors: H
authors: Jin
authors: Li
authors: L
authors: Liu
authors: Yan-Fei
authors: YF
authors: Zhang
authors: Jinsong
authors: J
authors: Liu
authors: Yan-Qing
authors: YQ
authors: Ding
authors: Jian-Ming
authors: JM
authors: Li
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics* / Q000378:metabolism*; D000818:Animals; D014408:Biomarkers, Tumor / Q000235:genetics; D015153:Blotting, Western / Q000379:methods; D045744:Cell Line, Tumor; D015179:Colorectal Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000556:secondary*; D015536:Down-Regulation; D005260:Female; D015870:Gene Expression; D020869:Gene Expression Profiling; D006801:Humans; D007150:Immunohistochemistry; D008113:Liver Neoplasms / Q000378:metabolism / Q000473:pathology / Q000556:secondary*; D008207:Lymphatic Metastasis; D008297:Male; D036961:Matrix Attachment Region Binding Proteins / Q000032:analysis / Q000235:genetics* / Q000378:metabolism; D051379:Mice; D008819:Mice, Nude; D008875:Middle Aged; D023421:Models, Animal; D009367:Neoplasm Staging; D009368:Neoplasm Transplantation; D020411:Oligonucleotide Array Sequence Analysis; D011379:Prognosis; D016016:Proportional Hazards Models; D034741:RNA, Small Interfering / Q000494:pharmacology; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D016019:Survival Analysis; D014157:Transcription Factors / Q000032:analysis / Q000235:genetics* / Q000378:metabolism; D014162:Transfection / Q000379:methods
chemical_list: D014408:Biomarkers, Tumor; D036961:Matrix Attachment Region Binding Proteins; D034741:RNA, Small Interfering; C521297:SATB2 protein, human; D014157:Transcription Factors