Microscopic investigation of reversible nanoscale surface size dependent protein conjugation.
Aβ(1-40) coated 20 nm gold colloidal nanoparticles exhibit a reversible color change as pH is externally altered between pH 4 and 10. This reversible process may contain important information on the initial reversible step reported for the fibrillogenesis of Aβ (a hallmark of Alzheimer's disease). We examined this reversible color change by microscopic investigations. AFM images on graphite surfaces revealed the morphology of Aβ aggregates with gold colloids. TEM images clearly demonstrate the correspondence between spectroscopic features and conformational changes of the gold colloid.
Yokoyama
Kazushige
K
Department of Chemistry, The State University of New York at Geneseo, 1 College Circle, Geneseo, NY 14454, USA.
Cho
Hyunah
H
Department of Chemistry, The State University of New York at Geneseo, 1 College Circle, Geneseo, NY 14454, USA.
Cullen
Sean P
SP
Department of Chemistry, The State University of New York at Geneseo, 1 College Circle, Geneseo, NY 14454, USA.
Kowalik
Matthew
M
Department of Chemistry, The State University of New York at Geneseo, 1 College Circle, Geneseo, NY 14454, USA.
Briglio
Nicole M
NM
Department of Chemistry, The State University of New York at Geneseo, 1 College Circle, Geneseo, NY 14454, USA.
Hoops
Harold J
HJ
Department of Biology, The State University of New York at Geneseo, 1 College Circle, Geneseo, NY 14454, USA.
Zhao
Zhouying
Z
College of Nanoscale Science and Engineering, University of Albany-SUNY, 255 Fuller Road, Albany, NY 12203, USA.
Carpenter
Michael A
MA
College of Nanoscale Science and Engineering, University of Albany-SUNY, 255 Fuller Road, Albany, NY 12203, USA.
D000544:Alzheimer Disease / Q000378:metabolism; D016229:Amyloid beta-Peptides / Q000096:biosynthesis / Q000737:chemistry*; D017979:Gold Colloid / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry*; D018625:Microscopy, Atomic Force; D046529:Microscopy, Electron, Transmission; D010047:Ovalbumin / Q000737:chemistry; D010446:Peptide Fragments / Q000096:biosynthesis / Q000737:chemistry*; D011487:Protein Conformation; D011506:Proteins / Q000378:metabolism; D014867:Water / Q000737:chemistry
D016229:Amyloid beta-Peptides; D017979:Gold Colloid; D010446:Peptide Fragments; D011506:Proteins; C078592:amyloid beta-protein (1-40); D014867:Water; D010047:Ovalbumin