Metallo-beta-lactamase-producing Pseudomonas spp. in Korea: high prevalence of isolates with VIM-2 type and emergence of isolates with IMP-1 type.
OBJECTIVE
Two Korean nationwide studies showed that metallo-beta-lactamases (MBLs)-producing-Pseudomonas spp. are not rare. The aim of this study was to assess the trends of MBL-producing isolates among imipenem-resistant isolates of Pseudomonas spp.


METHODS
Imipenem-resistant clinical isolates were collected from 23 hospitals and one commercial laboratory participating in the KONSAR program in 2005. Polymerase chain reaction (PCR) was used to detect MBL genes.


RESULTS
Alleles of MBL genes were detected in 10.8% of 415 Pseudomonas aeruginosa and 66.7% of 12 P. putida isolates from 18 of 24 hospitals/laboratory. Among the 14 IMP-1-like and 39 VIM-2-like MBLs, emergence of IMP-6 was detected for the first time.


CONCLUSIONS
Prevalence of MBL-producing P. aeruginosa has not significantly increased, but IMP-6 emerged in P. aeruginosa.
Lee
Kyungwon
K
Department of Laboratory Medicine, Yonsei University College of Medicine, 250 Seongsan-ro, Seodaemun-gu,Seoul, Korea.
Park
Ae Ja
AJ
Kim
Moon Yeun
MY
Lee
Hee Joo
HJ
Cho
Ji-Hyun
JH
Kang
Jung Oak
JO
Yong
Dongeun
D
Chong
Yunsop
Y
D000900:Anti-Bacterial Agents / Q000494:pharmacology; D016521:Electrophoresis, Gel, Pulsed-Field; D006801:Humans; D015378:Imipenem / Q000494:pharmacology; D007723:Korea; D016133:Polymerase Chain Reaction; D011552:Pseudomonas Infections / Q000382:microbiology*; D011550:Pseudomonas aeruginosa / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D001618:beta-Lactamases / Q000235:genetics / Q000378:metabolism*
D000900:Anti-Bacterial Agents; D015378:Imipenem; C409201:beta-lactamase IMP-1; C425108:beta-lactamase bla(vim-2); D001618:beta-Lactamases