[Therapeutic effects of traditional Chinese herbal formula with Smilax glabra on mice's lead poisoning].
OBJECTIVE
To investigate the influence of traditional Chinese herbal formula with Smilax glabra on lead expelling and oxidative damage in lead-poisoned mice.


METHODS
Mice were received intraperitoneal injections of lead acetate to establish lead-poisoned models. The concentration of lead, zinc and copper in blood, brain, liver, kidney and bone were determined. The lipid peroxidation of liver was systematically evaluated.


RESULTS
The traditional Chinese herbal formula had no influence on body weight and organ weight/body weight ratio of lead-poisoned mice. The traditional Chinese herbal formula might significantly reduce the concentration of lead in blood, rain, liver, kidney and bone, and the rate were 74%, 48%, 56%, 51% and 44%, but had no influence on zinc's and copper' s concentration. The decreasing rate of MDA levels in liver by the traditional Chinese herbal formula was 31%. In addition, the increase rate of GSH levels and the activities of GSH-Px and SOD in liver by traditional Chinese herbal formula were 63% ,76% and 66%, respectively.


CONCLUSIONS
The traditional Chinese herbal formula with Smilax glabra can significantly advance the excretion of lead and protect liver against oxidative damage induced by lead.
Xia
Dao-Zong
DZ
College of Pharmaceutical Sciences, Zhejiang Chinese Medical University, Hangzhou 310053, China. xiadaozong@zjtcm.net
Wang
Hui-Ming
HM
Yang
Qing
Q
Jiang
Li-Qin
LQ
Xia
Ming
M
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D001921:Brain / Q000187:drug effects / Q000378:metabolism; D004195:Disease Models, Animal; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D007668:Kidney / Q000187:drug effects / Q000378:metabolism; D007854:Lead / Q000097:blood* / Q000378:metabolism; D007855:Lead Poisoning / Q000188:drug therapy* / Q000378:metabolism; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D051379:Mice; D008813:Mice, Inbred ICR; D018384:Oxidative Stress / Q000187:drug effects*; D010946:Plants, Medicinal / Q000737:chemistry; D011897:Random Allocation; D032281:Smilax / Q000737:chemistry*
D000975:Antioxidants; D004365:Drugs, Chinese Herbal; D007854:Lead; D008315:Malondialdehyde