Effect of motif-programmed artificial proteins on the calcium uptake in a synthetic hydrogel.
Motif-programmed artificial proteins with mineralization-related activity were covalently immobilized onto the surface of a hydrogel, poly(2-hydroxyethyl methacrylate) (PHEMA). We investigated the influence of assaying conditions upon the ability of three selected proteins (PS64, PS382 and PS458) to modulate calcification in vitro. A long-term assay measuring the real amount of calcium phosphate phase in the protein-modified PHEMA showed that all proteins enhanced the uptake of calcium by the hydrogel. For PS382 and PS458, this is a behaviour opposite to that displayed when the same proteins were tested in a free state by a rapid solution assay. Such difference may be attributed to a restricted mobility of the proteins due to immobilization.
Chirila
Traian V
TV
Queensland Eye Institute, South Brisbane, Queensland 4101, Australia. traian.chirila@qei.org.au
Minamisawa
Tamiko
T
Keen
Imelda
I
Shiba
Kiyotaka
K
D000595:Amino Acid Sequence; D002118:Calcium / Q000378:metabolism*; D020100:Hydrogels / Q000737:chemistry / Q000378:metabolism; D008969:Molecular Sequence Data; D056951:Photoelectron Spectroscopy; D011102:Polyhydroxyethyl Methacrylate / Q000737:chemistry; D011487:Protein Conformation; D011506:Proteins / Q000737:chemistry* / Q000235:genetics; D013499:Surface Properties; D014961:X-Ray Diffraction
D020100:Hydrogels; D011506:Proteins; D011102:Polyhydroxyethyl Methacrylate; D002118:Calcium