title: Characterization of full-length VP4 genes of OP354-like P[8] human rotavirus strains detected in Bangladesh representing a novel P[8] subtype.
abstract: The G1 and G9 rotavirus strains MMC71 and MMC38 (subgroup II, NSP4 genogroup B), respectively, isolated from children in Bangladesh, were analyzed genetically. Full-length VP4 genes of these strains had 98.9% identity to each other and showed 83.9-89.4% identity to those of the P[4] and P[8] rotaviruses. Phylogenetic analysis of VP4 nucleotide sequences revealed that strains MMC38 and MMC71 were located in a lineage of P[8] strains. However, the cluster was highly divergent from the previously established P[8] strains. The VP8* portions of strains MMC38 and MMC71 showed more than 93.9% nucleotide sequence identity to OP354-like P[8] strains, and these strains were clustered into the same lineage. These findings indicate that the VP4 of these strains should be classified into a subtype of the P[8] genotype (P[8]b) that is distinct from that of common P[8] rotaviruses (P[8]a).
authors: Department of Hygiene, Sapporo Medical University School of Medicine, S-1 W-17 Chuo-ku, Sapporo, Hokkaido 060-8556, Japan. shigeon@sapmed.ac.jp
authors: Shigeo
authors: S
authors: Nagashima
authors: Nobumichi
authors: N
authors: Kobayashi
authors: Shyamal Kumar
authors: SK
authors: Paul
authors: Mohammed Mahbub
authors: MM
authors: Alam
authors: Mamta
authors: M
authors: Chawla-Sarkar
authors: Triveni
authors: T
authors: Krishnan
mesh_terms: D001459:Bangladesh / Q000453:epidemiology; D036022:Capsid Proteins / Q000235:genetics*; D002648:Child; D005814:Genes, Viral; D006801:Humans; D008969:Molecular Sequence Data; D012401:Rotavirus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D012400:Rotavirus Infections / Q000453:epidemiology* / Q000821:virology; D017386:Sequence Homology, Amino Acid
chemical_list: D036022:Capsid Proteins; C060339:VP4 protein, Rotavirus