Sulfonation and anticoagulant activity of botryosphaeran from Botryosphaeria rhodina MAMB-05 grown on fructose.
Botryosphaeran (EPS(FRU)), an exopolysaccharide of the beta-(1-->3,1-->6)-d-glucan type with 31% branching at C-6, is produced by the fungus Botryosphaeria rhodina MAMB-05 when grown on fructose as carbon source. Botryosphaeran was derivatized by sulfonation to induce anticoagulant activity. The effectiveness of the sulfonation reaction by chlorosulfonic acid in pyridine was monitored by the degree of substitution and FT-IR analysis of the sulfonated EPS(FRU) (once sulfonated, EPS(FRUSULF); and re-sulfonated, EPS(FRURESULF)). Activated partial thromboplastin time (APTT) and thrombin time (TT) tests of EPS(FRURESULF) indicated significant in vitro anticoagulant activity that was dose-dependent. EPS(FRU) did not inhibit any of the coagulation tests.
Mendes
Simone Ferreira
SF
Dept(o) de Física, Química e Biologia, FCT, Universidade Estadual Paulista, CEP 19060-900, Presidente Prudente - São Paulo, Brazil.
dos Santos
Osvaldo
O
Barbosa
Aneli M
AM
Vasconcelos
Ana Flora D
AF
Aranda-Selverio
Gabriel
G
Monteiro
Nilson K
NK
Dekker
Robert F H
RF
Sá Pereira
Mariana
M
Tovar
Ana Maria F
AM
Mourão
Paulo A de Souza
PA
da Silva
Maria de Lourdes Corradi
Mde L
D000925:Anticoagulants / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D001203:Ascomycota / Q000254:growth & development* / Q000378:metabolism*; D001777:Blood Coagulation / Q000187:drug effects; D005632:Fructose / Q000378:metabolism*; D005936:Glucans / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology*; D006493:Heparin / Q000494:pharmacology; D006801:Humans; D017550:Spectroscopy, Fourier Transform Infrared; D013451:Sulfonic Acids / Q000378:metabolism*
D000925:Anticoagulants; D005936:Glucans; D013451:Sulfonic Acids; C483238:botryosphaeran; D005632:Fructose; D006493:Heparin