title: Analysis of intestinal injuries induced by ricin in vitro using SPR technology and MS identification.
abstract: The present study found that ricin toxicity did not only manifest itself as inhibition of protein synthesis, but also induced apoptosis of immune cells and played an extremely significant role in intestinal injury. In this report, we describe a novel method to estimate binding events occurring on intestinal brush border membranes (BBM) based on SPR technology in an attempt to mimic the real intestinal surface capable of interacting physically and/or actively with certain biological molecules. Combined with HPCE-ESI-MS indentification, we obtained 28 kinds of proteins in BBM that interacted with ricin.
authors: Institute of Military Veterinary, Academy of Military Medical Science, Changchun 130062, China.|Department of Theoretic Veterinary Medicine, Colledge of Animal Science and Veterinary, Jilin University, Changchun 130062, China.
authors: Linna
authors: L
authors: Liu
authors: Institute of Military Veterinary, Academy of Military Medical Science, Changchun 130062, China.
authors: Hongwei
authors: H
authors: Gao
authors: Institute of Military Veterinary, Academy of Military Medical Science, Changchun 130062, China.
authors: Jiping
authors: J
authors: Li
authors: Institute of Military Veterinary, Academy of Military Medical Science, Changchun 130062, China.
authors: Ying
authors: Y
authors: Dong
authors: Institute of Military Veterinary, Academy of Military Medical Science, Changchun 130062, China.
authors: Ning
authors: N
authors: Liu
authors: Institute of Military Veterinary, Academy of Military Medical Science, Changchun 130062, China.
authors: Jiayu
authors: J
authors: Wan
authors: Institute of Military Veterinary, Academy of Military Medical Science, Changchun 130062, China.
authors: Wensen
authors: W
authors: Liu
authors: Institute of Military Veterinary, Academy of Military Medical Science, Changchun 130062, China.
authors: Yucheng
authors: Y
authors: Sun
authors: Institute of Military Veterinary, Academy of Military Medical Science, Changchun 130062, China.
authors: Ming
authors: M
authors: Xu
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D015374:Biosensing Techniques; D007413:Intestinal Mucosa / Q000187:drug effects*; D007422:Intestines / Q000187:drug effects / Q000293:injuries*; D013058:Mass Spectrometry; D008567:Membranes, Artificial; D051379:Mice; D008871:Microvilli / Q000187:drug effects* / Q000378:metabolism; D014176:Protein Biosynthesis / Q000187:drug effects; D051381:Rats; D017207:Rats, Sprague-Dawley; D012276:Ricin / Q000378:metabolism / Q000633:toxicity*; D020349:Surface Plasmon Resonance
chemical_list: D008567:Membranes, Artificial; D012276:Ricin