title: Lipid nanocapsules: ready-to-use nanovectors for the aerosol delivery of paclitaxel.
abstract: Aerosol drug delivery permits the development of dose-intensification strategies in severe, malignant lung diseases. The aim of the study was to demonstrate that the encapsulation of paclitaxel in lipid nanocapsules (LNCs), a novel drug nanocarrier for lipophilic components, allows one to provide pulmonary drug delivery of paclitaxel by nebulisation, thereby allowing preclinical and clinical studies. LNC dispersions are made into aerosols with commercial nebulisers. The structure, drug payload and cytotoxicity of nebulised LNCs were compared to fresh LNCs. The results demonstrated that LNC dispersions could be made into aerosols by using mesh nebulisers without altering the LNC structure. Only eFlow rapid-produced aerosols are compatible with human use: the mean duration to nebulise 3 ml of LNC dispersion is less than 9 min, with an aerosol mass median aerodynamic diameter equal to 2.7+/-0.1 microm and a fine-particle fraction (between 1.0 and 5.0 microm) of 81.5+/-3.1%. No modifications of drug payload or cytotoxicity effects of paclitaxel-loaded LNC (PTX-LNC) were observed. In order to carry out preclinical studies, a scaled-up LNC formulation protocol was used. Chemical parameters, such as acidity and osmolarity, were optimised, and a storage procedure for PTX-LNC batches was set-up. Animal studies are now needed to determine the tolerance and therapeutic potential of LNC dispersion aerosols.
authors: Pulmonology Department, Academic Hospital, Angers, France. jose.hureaux@inserm.fr
authors: José
authors: J
authors: Hureaux
authors: Frédéric
authors: F
authors: Lagarce
authors: Frédéric
authors: F
authors: Gagnadoux
authors: Laurent
authors: L
authors: Vecellio
authors: Anne
authors: A
authors: Clavreul
authors: Emilie
authors: E
authors: Roger
authors: Marie
authors: M
authors: Kempf
authors: Jean-Louis
authors: JL
authors: Racineux
authors: Patrice
authors: P
authors: Diot
authors: Jean-Pierre
authors: JP
authors: Benoit
authors: Thierry
authors: T
authors: Urban
mesh_terms: D000280:Administration, Inhalation; D000336:Aerosols; D000972:Antineoplastic Agents, Phytogenic / Q000008:administration & dosage / Q000737:chemistry*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002626:Chemistry, Pharmaceutical; D004305:Dose-Response Relationship, Drug; D004337:Drug Carriers; D004339:Drug Compounding; D004355:Drug Stability; D004356:Drug Storage; D005240:Feasibility Studies; D006801:Humans; D006863:Hydrogen-Ion Concentration; D020128:Inhibitory Concentration 50; D008055:Lipids / Q000737:chemistry*; D008175:Lung Neoplasms / Q000473:pathology; D053769:Nanocapsules; D009330:Nebulizers and Vaporizers; D009994:Osmolar Concentration; D017239:Paclitaxel / Q000008:administration & dosage / Q000737:chemistry*; D010316:Particle Size; D012995:Solubility
chemical_list: D000336:Aerosols; D000972:Antineoplastic Agents, Phytogenic; D004337:Drug Carriers; D008055:Lipids; D053769:Nanocapsules; D017239:Paclitaxel