Promicromonospora flava sp. nov., isolated from sediment of the Baltic Sea.
A Gram-positive, non-spore-forming actinomycete, designated strain CC 0387(T), was isolated from a sediment sample from the Baltic Sea, Germany. Classification using a polyphasic approach and comparative 16S rRNA gene sequencing showed that strain CC 0387(T) belonged to the genus Promicromonospora and displayed more than 3 % 16S rRNA gene sequence divergence from all Promicromonospora species with validly published names. Strain CC 0387(T) did not produce aerial mycelium. Substrate mycelia were yellowish white to pale orange-yellow and fragmented into bacillary or coccoid elements. The cell wall contained lysine and alanine. Whole-cell hydrolysates contained galactose, glucose, rhamnose and ribose. The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylglycerol, an unknown phospholipid and an unknown glycolipid. The predominant menaquinone was MK-9(H(4)) (86 %). The G+C content of the DNA was 71.87 mol%. Based on morphological, chemotaxonomic, phenotypic and genetic characteristics, strain CC 0387(T) (=CCTCC AA208024(T)=DSM 21481(T)) represents a novel species, for which the name Promicromonospora flava sp. nov. is proposed.
Jiang
Yi
Y
Leibniz-Institut für Meereswissenschaften, IFM-GEOMAR, Düsternbrooker Weg 20, D-24105 Kiel, Germany.
Wiese
Jutta
J
Cao
Yan-Ru
YR
Xu
Li-Hua
LH
Imhoff
Johannes F
JF
Jiang
Cheng-Lin
CL
D000192:Actinomycetales / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology; D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000032:analysis; D005227:Fatty Acids / Q000032:analysis; D020459:Genes, rRNA; D005838:Genotype; D019015:Geologic Sediments / Q000382:microbiology*; D005858:Germany; D008969:Molecular Sequence Data; D010641:Phenotype; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012623:Seawater / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D013045:Species Specificity
D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S