title: Confocal laser endomicroscopy in Barrett's esophagus and endoscopically inapparent Barrett's neoplasia: a prospective, randomized, double-blind, controlled, crossover trial.
abstract: BACKGROUND
  The detection of high-grade dysplasia and cancer in Barrett's esophagus (BE) can be challenging. Confocal laser endomicroscopy (CLE) allows in vivo visualization of mucosal histology during endoscopy.
  
  
  OBJECTIVE
  To determine whether CLE with optical biopsy and targeted mucosal biopsy improves the diagnostic yield of endoscopically inapparent, BE-associated neoplasia compared to standard endoscopy with a 4-quadrant, random biopsy protocol.
  
  
  METHODS
  Prospective, double-blind, randomized, crossover study.
  
  
  METHODS
  Single, tertiary-care academic center.
  
  
  METHODS
  This study involved patients with BE undergoing routine surveillance or referred for treatment of nonlocalized, endoscopically inapparent, BE-associated neoplasia.
  
  
  METHODS
  All participants underwent both a confocal endomicroscopy with a targeted biopsy procedure and standard endoscopy with a 4-quadrant biopsy procedure in a randomized order.
  
  
  METHODS
  Increase in diagnostic yield for neoplasia, reduction in mucosal biopsy number, final pathologic diagnosis.
  
  
  RESULTS
  CLE with targeted biopsy almost doubled the diagnostic yield for neoplasia and was equivalent to the standard protocol for the final diagnosis of neoplasia. Two thirds of patients in the surveillance group did not need any mucosal biopsies at all.
  
  
  CONCLUSIONS
  Single-center study.
  
  
  CONCLUSIONS
  CLE with targeted biopsy significantly improves the diagnostic yield for endoscopically inapparent BE neoplasia compared to a standard endoscopy with a random-biopsy protocol. CLE with targeted biopsy also greatly reduces the number of biopsies needed per patient and allows some patients without neoplasia to completely forgo mucosal biopsy.
authors: Division of Gastroenterology and Hepatology, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA. kdunbar@jhmi.edu
authors: Kerry B
authors: KB
authors: Dunbar
authors: Patrick
authors: P
authors: Okolo
authors: Elizabeth
authors: E
authors: Montgomery
authors: Marcia Irene
authors: MI
authors: Canto
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001471:Barrett Esophagus / Q000473:pathology*; D001706:Biopsy; D018592:Cross-Over Studies; D004311:Double-Blind Method; D004938:Esophageal Neoplasms / Q000473:pathology*; D004945:Esophagoscopy; D005260:Female; D006801:Humans; D008297:Male; D018613:Microscopy, Confocal; D008875:Middle Aged; D011446:Prospective Studies