title: Wood dusts induce the production of reactive oxygen species and caspase-3 activity in human bronchial epithelial cells.
abstract: Wood dusts are associated with several respiratory symptoms, e.g. impaired lung function and asthma, in exposed workers. However, despite the evidence from epidemiological studies, the underlying mechanisms are not well understood. In the present study, we investigated different wood dusts for their capacity to induce cytotoxicity and production of radical oxygen species (ROS) as well as activation of the apoptotic caspase-3 enzyme in human bronchial epithelial cells (BEAS-2B). Dusts from three different tree species widely used in wood industry were studied; birch and oak represented hardwood species, and pine a common softwood species. All the experiments were carried out in three different concentrations (10, 50, and 500 microg/ml) and the analysis was performed after 0.5, 2, 6, and 24h exposure. All wood dusts studied were cytotoxic to human bronchial epithelial cells in a dose-dependent manner after 2 and 6h treatment. Exposure to pine, birch, or oak dust had a significant stimulating effect on the production of ROS. Also an induction in caspase-3 protease activity, one of the central components of the apoptotic cascade, was seen in BEAS-2B cells after 2 and 6h exposure to each of the wood dusts studied. In summary, we demonstrate that dusts from pine, birch and oak are cytotoxic, able to increase the production of ROS and the apoptotic response in human broncho-epithelial cells in vitro. Thus, our current data suggest oxidative stress by ROS as an important mechanism likely to function in wood dust related pulmonary toxicity although details of the cellular targets and cell-particle interactions remain to be solved. It is though tempting to speculate that redox-regulated transcription factors such as NFkappaB or AP-1 may play a role in this wood dust-evoked process leading to apparently induced apoptosis of target cells.
authors: Finnish Institute of Occupational Health, Topeliuksenkatu 41 a, 00280 Helsinki, Finland. lea.pylkkanen@ttl.fi
authors: Lea
authors: L
authors: Pylkkänen
authors: Helene
authors: H
authors: Stockmann-Juvala
authors: Harri
authors: H
authors: Alenius
authors: Kirsti
authors: K
authors: Husgafvel-Pursiainen
authors: Kai
authors: K
authors: Savolainen
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D029662:Betula / Q000737:chemistry; D001980:Bronchi / Q000166:cytology; D053148:Caspase 3 / Q000378:metabolism*; D002460:Cell Line; D004305:Dose-Response Relationship, Drug; D004391:Dust; D004847:Epithelial Cells / Q000378:metabolism; D005387:Finland; D006801:Humans; D018384:Oxidative Stress; D028223:Pinus / Q000737:chemistry; D029963:Quercus / Q000737:chemistry; D017382:Reactive Oxygen Species / Q000378:metabolism*; D013997:Time Factors; D014934:Wood / Q000737:chemistry / Q000633:toxicity*
chemical_list: D004391:Dust; D017382:Reactive Oxygen Species; D053148:Caspase 3