Survey of feline leukemia virus and feline coronaviruses in captive neotropical wild felids from Southern Brazil.
A total of 57 captive neotropical felids (one Leopardus geoffroyi, 14 Leopardus pardalis, 17 Leopardus wiedii, 22 Leopardus tigrinus, and three Puma yagouaroundi) from the Itaipu Binacional Wildlife Research Center (Refúgio Bela Vista, Southern Brazil) were anesthetized for blood collection. Feces samples were available for 44 animals, including one L. geoffroyi, eight L. pardalis, 14 L. wiedii, 20 L. tigrinus, and one P. yagouaroundi. Total DNA and RNA were extracted from blood and feces, respectively, using commercial kits. Blood DNA samples were evaluated by polymerase chain reaction (PCR) for feline leukemia virus (FeLV) proviral DNA, whereas reverse transcriptase-PCR was run on fecal samples for detection of coronavirus RNA. None of the samples were positive for coronaviruses. A male L. pardalis and a female L. tigrinus were positive for FeLV proviral DNA, and identities of PCR products were confirmed by sequencing. This is the first evidence of FeLV proviral DNA in these species in Southern Brazil.
Guimaraes
Ana M S
AM
Instituto de Ciências Biomédicas, USP, Av. Prof. Lineu Prestes, 1374, 05508900, São Paulo, Brazil.
Brandão
Paulo E
PE
de Moraes
Wanderlei
W
Cubas
Zalmir S
ZS
Santos
Leonilda C
LC
Villarreal
Laura Y B
LY
Robes
Rogério R
RR
Coelho
Fabiana M
FM
Resende
Mauricio
M
Santos
Renata C F
RC
Oliveira
Rosangela C
RC
Yamaguti
Mauricio
M
Marques
Lucas M
LM
Neto
Renata L
RL
Buzinhani
Melissa
M
Marques
Regina
R
Messick
Joanne B
JB
Biondo
Alexander W
AW
Timenetsky
Jorge
J
D000818:Animals; D000835:Animals, Wild; D000836:Animals, Zoo; D001938:Brazil / Q000453:epidemiology; D018352:Coronavirus Infections / Q000175:diagnosis / Q000453:epidemiology / Q000662:veterinary*; D016765:Coronavirus, Feline / Q000302:isolation & purification; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics; D005243:Feces / Q000821:virology; D045989:Felidae / Q000821:virology*; D005260:Female; D005256:Leukemia Virus, Feline / Q000302:isolation & purification; D008297:Male; D016133:Polymerase Chain Reaction / Q000662:veterinary; D015995:Prevalence; D046011:Puma / Q000821:virology*; D012192:Retroviridae Infections / Q000175:diagnosis / Q000453:epidemiology / Q000662:veterinary*; D013045:Species Specificity; D014412:Tumor Virus Infections / Q000175:diagnosis / Q000453:epidemiology / Q000662:veterinary*
D004279:DNA, Viral