Porcine skin as a model system for studies of adverse effects of narrow-band UVB pulses on human skin.
Ultraviolet (UV) radiation has been widely used in medicine, and in recent years there has been a growing interest in narrow-band UVB therapies, especially those employing pulses of the 308-nm line of XeCl excimer lasers. Comparative studies in several skin pathologies showed that narrow-band UVB was more effective than classical broad-band UVB radiation. Simultaneously, UVB is carcinogenic and there is a need for data to establish the risk associated with phototherapies involving irradiations of human skin with different doses of narrow- and broad-band UVA and/or UVB radiation. Relevant data are sparse predominantly due to a lack of suitable model systems for study of this phenomenon. Our comparative study of human and porcine skin responses to pulses of narrow-band UVB radiation demonstrated that for doses ranging from 5 to 10,000 mJ/cm(2) both skin types have similar susceptibility to UVB-induced breaking of nuclear DNA, indicating that pig skin might serve as good model for studies of sensitivity of human skin to UVB radiation.
Brozyna
Anna
A
Department of Medical Biology, Institute of General and Molecular Biology, Nicolaus Copernicus University, Toruń, Poland.
Wasilewska
Katarzyna
K
Wesierska
Katarzyna
K
Chwirot
Barbara W
BW
D000818:Animals; D004307:Dose-Response Relationship, Radiation; D006801:Humans; D012867:Skin / Q000473:pathology / Q000528:radiation effects*; D013552:Swine / Q000502:physiology*; D014466:Ultraviolet Rays / Q000009:adverse effects*