title: Evolutionary characterization of a highly repetitive sequence identified from the false killer whale (Pseudorca crassidens).
abstract: We report here that a novel 1,869 bp repetitive sequence identified from the false killer whale (Pseudorca crassidens) could be a new molecular phylogenetic marker in cetaceans. Results of PCR amplification and southern blot hybridization using 16 species' genomic DNAs from five different families revealed that the repetitive sequence is highly conserved within all Delphinidae species. Notably, specific primers designed for this repetitive sequence effectively amplified the targeted repetitive units, which were critically dependent upon the genetic phylogenies in the members of the Delphinidae cetaceans. Therefore, the novel sequences can be used as a useful phylogenetic marker for understanding the molecular evolutional studies in members of the Delphinidae family of cetaceans.
authors: Genome Research Center, Korea Research Institute of Bioscience and Biotechnology, Baejeon, Korea.
authors: Dae-Won
authors: DW
authors: Kim
authors: Aram
authors: A
authors: Kang
authors: Sang-Haeng
authors: SH
authors: Choi
authors: Zang Geun
authors: ZG
authors: Kim
authors: Woo-Jin
authors: WJ
authors: Kim
authors: Hyung-Cheol
authors: HC
authors: Kim
authors: Hong-Seog
authors: HS
authors: Park
mesh_terms: D000818:Animals; D001483:Base Sequence; D002591:Cetacea / Q000145:classification / Q000235:genetics; D004272:DNA, Mitochondrial / Q000737:chemistry / Q000235:genetics; D004289:Dolphins / Q000145:classification / Q000235:genetics*; D019143:Evolution, Molecular; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics*; D017422:Sequence Analysis, DNA
chemical_list: D004272:DNA, Mitochondrial; D012336:RNA, Ribosomal, 16S