title: Repeatability and reproducibility of macular thickness measurements using fourier domain optical coherence tomography.
abstract: OBJECTIVE
  To evaluate repeatability and reproducibility of macular thickness measurements in visually normal eyes using the Topcon 3D OCT-1000.
  
  
  METHODS
  Phase 1 investigated scan repeatability, the effect of age and pupil dilation. Two groups (6 younger and 6 older participants) had one eye scanned 5 times pre and post- dilation by 1 operator. Phase 2 investigated between-operator, within and between-visit reproducibility. 10 participants had 1 un-dilated eye scanned 3 times on 2 separate visits by 2 operators.
  
  
  RESULTS
  PHASE 1: No significant difference existed between repeat scans (p=0.75) and no significant difference was found pre- and post-dilation (p=0.54). In the younger group variation was low (95% limits +/- 3.62microm) and comparable across all retinal regions. The older group demonstrated greater variation (95% limits +/- 7.6microm). PHASE 2: For a given retinal location, 95% confidence limits for within-operator, within-visit reproducibility was 5.16microm. This value increased to 5.56microm for the same operator over two visits and to 6.18microm for two operators over two visits.
  
  
  CONCLUSIONS
  A high level repeatability, close to 6microm, of macular thickness measurement is possible using the 3D OCT- 1000. Measured differences in macular thickness between successive visits that exceed 6microm in pre-presbyopic individuals are therefore likely to reflect actual structural change. OCT measures are more variable in older individuals and it is advisable to take a series of scans so that outliers can be more easily identified.
authors: Bradford School of Optometry and Vision Science, University of Bradford, Bradford, UK.
authors: Alison
authors: A
authors: Bruce
authors: Ian E
authors: IE
authors: Pacey
authors: Poonam
authors: P
authors: Dharni
authors: Andy J
authors: AJ
authors: Scally
authors: Brendan T
authors: BT
authors: Barrett