A strip-shield improves the efficiency of a solenoid coil in probes for high-field solid-state NMR of lossy biological samples.
A strip-shield inserted between a high inductance double-tuned solenoid coil and the glass tube containing the sample improves the efficiency of probes used for high-field solid-state NMR experiments on lossy aqueous samples of proteins and other biopolymers. A strip-shield is a coil liner consisting of thin copper strips layered on a PTFE (polytetrafluoroethylene) insulator. With lossy samples, the shift in tuning frequency is smaller, the reduction in Q, and RF-induced heating are all significantly reduced when the strip-shield is present. The performance of 800MHz (1)H/(15)N and (1)H/(13)C double-resonance probes is demonstrated on aqueous samples of membrane proteins in phospholipid bilayers.
Wu
Chin H
CH
Department of Chemistry and Biochemistry, 9500 Gilman Drive, University of California, San Diego, La Jolla, CA 92093-0307, USA.
Grant
Christopher V
CV
Cook
Gabriel A
GA
Park
Sang Ho
SH
Opella
Stanley J
SJ
D001704:Biopolymers / Q000032:analysis*; D002247:Carbon Isotopes / Q000737:chemistry; D003198:Computer Simulation; D004574:Electromagnetic Fields; D004867:Equipment Design; D006859:Hydrogen / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy / Q000295:instrumentation*; D009587:Nitrogen Isotopes / Q000737:chemistry; D011138:Polytetrafluoroethylene; D025941:Protein Interaction Mapping / Q000379:methods; D011506:Proteins / Q000032:analysis*; D014764:Viral Proteins / Q000737:chemistry; D000086082:Viroporin Proteins
D001704:Biopolymers; D002247:Carbon Isotopes; D006859:Hydrogen; D009587:Nitrogen Isotopes; D011138:Polytetrafluoroethylene; D011506:Proteins; D014764:Viral Proteins; C088369:p7 protein, Hepatitis C virus; D000086082:Viroporin Proteins