Metabolomic profiling of rat serum associated with isoproterenol-induced myocardial infarction using ultra-performance liquid chromatography/time-of-flight mass spectrometry and multivariate analysis.
An ultra-performance liquid chromatography/time-of-flight mass spectrometry (UPLC/TOF-MS)-based metabolomic approach was developed to characterize the metabolic profile associated with isoproterenol (ISO)-induced myocardial infarction (MI). Analysis of the serum samples revealed distinct changes in the biochemical patterns of ISO-induced rats. A multivariate statistical method, supervised partial least squares-discriminant analysis (PLS-DA), was then used for screening of potential biomarkers. As a result, 13 lipid biomarkers, including lysophosphatidylcholines (Lyso-PCs) and fatty acids were identified by the accurate mass measurement of TOF-MS. The relationship between abnormal lipid metabolism and the formation of MI were also studied. This work demonstrates the utility of UPLC/TOF-MS-based metabolic profiling combined with multivariate analysis as a powerful tool to further investigate the pathogenesis of cardiovascular diseases.
Zhang
Hong-yang
HY
School of Chemistry and Molecular Engineering, East-China University of Science and Technology, Shanghai 200237, China.
Chen
Xi
X
Hu
Ping
P
Liang
Qiong-lin
QL
Liang
Xiao-ping
XP
Wang
Yi-ming
YM
Luo
Guo-an
GA
D000818:Animals; D015415:Biomarkers; D002851:Chromatography, High Pressure Liquid; D007545:Isoproterenol / Q000633:toxicity; D050356:Lipid Metabolism; D008055:Lipids / Q000097:blood; D055442:Metabolome; D055432:Metabolomics; D015999:Multivariate Analysis; D009203:Myocardial Infarction / Q000097:blood* / Q000139:chemically induced / Q000378:metabolism; D051381:Rats; D044967:Serum / Q000378:metabolism*; D053719:Tandem Mass Spectrometry
D015415:Biomarkers; D008055:Lipids; D007545:Isoproterenol