Evaluation of total oxidative and antioxidative status in pediatric patients undergoing laparoscopic surgery.
OBJECTIVE
Reactive oxygen species (ROS) induced by several diseases can trigger oxidative stress. During laparoscopy, increased intraabdominal pressure caused by pneumoperitoneum may lead to splanchnic ischemia followed by reperfusion because of deflation. Because ischemia reperfusion creates oxidative stress, in this study, we aimed to investigate the oxidative-antioxidative status of the pediatric patients with laparoscopic surgery.


METHODS
The children underwent laparoscopic procedures under general anesthesia, and they were mechanically ventilated. Blood samples were obtained after induction of anesthesia, at the end of the surgery, and were centrifuged at 3000 revolutions per minute for 10 minutes to separate plasma. The plasma total antioxidant status (TAS), total oxidant status (TOS), and oxidative stress index (OSI) were determined.


RESULTS
Plasma TOS and OSI levels were significantly higher at the end of the surgery than after induction of anesthesia (P < .05 and P < .01, respectively). On the other hand, plasma TAS levels were lower in the end of the surgery than after induction of anesthesia (P < .05).


CONCLUSIONS
These results suggest that ROS are generated during the laparoscopic procedure, possibly as a result of an ischemia-reperfusion phenomenon induced by the inflation and deflation of the pneumoperitoneum, which causes oxidative stress and consume plasma antioxidants.
Baysal
Zeynep
Z
Department of Anesthesiology and Reanimation, Faculty of Medicine, Harran University, 63100 Sanliurfa, Turkey. zbaysal2003@yahoo.com
Togrul
Turhan
T
Aksoy
Nurten
N
Cengiz
Mustafa
M
Celik
Hakim
H
Boleken
M Emin
ME
Kaya
Mete
M
Yavuz
Galip
G
D000975:Antioxidants / Q000378:metabolism*; D002675:Child, Preschool; D017558:Elective Surgical Procedures / Q000379:methods*; D006801:Humans; D010535:Laparoscopy / Q000379:methods*; D018384:Oxidative Stress / Q000502:physiology*; D011300:Preoperative Care / Q000379:methods*; D011446:Prospective Studies; D017382:Reactive Oxygen Species / Q000097:blood*; D012307:Risk Factors
D000975:Antioxidants; D017382:Reactive Oxygen Species