Fluorescence two-dimensional difference gel electrophoresis for biomaterial applications.
Fluorescence two-dimensional difference gel electrophoresis (DiGE) is rapidly becoming established as a powerful technique for the characterization of differences in protein expression levels between two or more conditions. In this review, we consider the application of DiGE-both minimal and saturation labelling-to biomaterials research, considering the challenges and rewards of this approach.
McNamara
Laura E
LE
Centre for Cell Engineering, University of Glasgow, Glasgow G12 8QQ, UK. l.mcnamara.1@research.gla.ac.uk
Dalby
Matthew J
MJ
Riehle
Mathis O
MO
Burchmore
Richard
R
D001672:Biocompatible Materials / Q000737:chemistry*; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods*; D005453:Fluorescence; D011506:Proteins / Q000302:isolation & purification*; D040901:Proteomics / Q000379:methods*; D013194:Staining and Labeling / Q000379:methods
D001672:Biocompatible Materials; D011506:Proteins