title: Cellulase production from Aspergillus niger MS82: effect of temperature and pH.
abstract: Fungal cellulases are well-studied enzymes and are used in various industrial processes. Much of the knowledge of enzymatic depolymerization of cellulosic material has come from Trichoderma cellulase system. Species of Trichoderma can produce substantial amounts of endoglucanase and exoglucanase but very low levels of b-glucosidase. This deficiency necessitates screening of fungi for cellulytic potential. A number of indigenously isolated fungi were screened for cellulytic potential. In the present study, the kinetics of cellulase production from an indigenous strain of Aspergillus niger MS82 is reported. Product formation parameters of endoglucanase and beta-glucosidase (Qp + Y(p/s)) indicate that A.niger MS82 is capable of producing moderate to high levels of both endoglucanase and beta-glucosidase when grown on different carbon containing natural substrates, for example, grass, corncob, bagasse along side purified celluloses. Furthermore, it was observed that the production of endoglucanase reaches its maximum during exponential phase of growth, while b-glucosidase during the Stationary phase. Enzyme production by solid-state fermentation was also investigated and found to be promising.Highest production of cellulase was noted at pH 4.0 at 35 degrees C under submerged conditions. Growth and enzyme production was affected by variations in temperature and pH.
authors: Department of Microbiology, University of Karachi, Karachi 75270, Pakistan.
authors: Muhammad
authors: M
authors: Sohail
authors: Roquya
authors: R
authors: Siddiqi
authors: Aqeel
authors: A
authors: Ahmad
authors: Shakeel Ahmed
authors: SA
authors: Khan
mesh_terms: D001234:Aspergillus niger / Q000254:growth & development* / Q000378:metabolism*; D019149:Bioreactors / Q000382:microbiology*; D018929:Cell Culture Techniques / Q000379:methods*; D002480:Cellulase / Q000096:biosynthesis*; D006863:Hydrogen-Ion Concentration; D013696:Temperature
chemical_list: D002480:Cellulase