title: Polyamines affect histamine synthesis during early stages of IL-3-induced bone marrow cell differentiation.
abstract: Mast cells synthesize and store histamine, a key immunomodulatory mediator. Polyamines are essential for every living cell. Previously, we detected an antagonistic relationship between the metabolisms of these amines in established mast cell and basophilic cell lines. Here, we used the IL-3-driven mouse bone marrow-derived mast cell (BMMC) culture system to further investigate this antagonism in a mast cell model of deeper physiological significance. Polyamines and histamine levels followed opposite profiles along the bone marrow cell cultures leading to BMMCs. alpha-Difluoromethylornithine (DFMO)-induced polyamine depletion resulted in an upregulation of histidine decarboxylase (HDC, the histamine-synthesizing enzyme) expression and activity, accompanied by increased histamine levels, specifically during early stages of these cell cultures, where an active histamine synthesis process occurs. In contrast, DFMO did not induce any effect in either HDC activity or histamine levels of differentiated BMMCs or C57.1 mast cells, that exhibit a nearly inactive histamine synthesis rate. Sequence-specific DNA methylation analysis revealed that the DFMO-induced HDC mRNA upregulation observed in early bone marrow cell cultures is not attributable to a demethylation of the gene promoter caused by the pharmacological polyamine depletion. Taken together, the results support an inverse relationship between histamine and polyamine metabolisms during the bone marrow cell cultures leading to BMMCs and, moreover, suggest that the regulation of the histamine synthesis occurring during the early stages of these cultures depends on the concentrations of polyamines.
authors: Faculty of Sciences, Department of Molecular Biology, University of Málaga, CIBER de Enfermedades Raras (CIBER-ER), Campus de Teatinos s/n, 29071 Málaga, Spain.
authors: Gianni
authors: G
authors: García-Faroldi
authors: Florencia
authors: F
authors: Correa-Fiz
authors: Hicham
authors: H
authors: Abrighach
authors: María
authors: M
authors: Berdasco
authors: Mario F
authors: MF
authors: Fraga
authors: Manel
authors: M
authors: Esteller
authors: José L
authors: JL
authors: Urdiales
authors: Francisca
authors: F
authors: Sánchez-Jiménez
authors: Ignacio
authors: I
authors: Fajardo
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology* / Q000378:metabolism; D002454:Cell Differentiation; D002478:Cells, Cultured; D019175:DNA Methylation; D006632:Histamine / Q000096:biosynthesis*; D006640:Histidine Decarboxylase / Q000235:genetics / Q000378:metabolism; D007377:Interleukin-3 / Q000378:metabolism*; D008407:Mast Cells / Q000166:cytology / Q000378:metabolism; D051379:Mice; D011073:Polyamines / Q000494:pharmacology*
chemical_list: D007377:Interleukin-3; D011073:Polyamines; D006632:Histamine; D006640:Histidine Decarboxylase