title: Contact allergy to nickel: patch test score correlates with IL-5, but not with IFN-gamma nickel-specific secretion by peripheral blood lymphocytes.
abstract: Traditionally, allergic contact dermatitis (ACD) has been associated with the activity of Th1 lymphocytes that secrete interferon gamma. Recent evidence indicates that other cells, e.g. interleukin 5 (IL-5)-secreting Th2 or Tc2 cells may be among the key effectors of ACD. The aim of the present study was to assess the influence of nickel-specific IFN-gamma secretion (marker of Th1 and Tc1 activity) and IL-5 secretion (Th2 and Tc2) on the clinical outcome (patch test score) in nickel-allergic patients. 40 women with suspicion of ACD were involved, aged from 14-54 (median 31.5) years. They were patch tested with NiSO(4). Peripheral blood mononuclear cells (PBMC) from the patients were cultured and analysed for IFN-gamma and IL-5 secretion in response to NiSO(4). A series of statistical models (classical logit or cloglog link function) were used. We demonstrate that nickel-specific IL-5 secretion by PBMC is correlated with the intensity of patch test reaction (p=0.05), with no significant effect of IFN-gamma. An increase in the nickel-specific IL-5 secretion from PBMC by 10 pg/ml is associated with a 10-20% increase (depending on statistical model) in the odds ratio of the patient to have a higher patch test score. These findings support the assumption that cells secreting IL-5 (e.g. Th2, Tc2) play a more important role in the pathogenesis of ACD than previously thought.
authors: Institute of Clinical and Environmental Toxicology, Faculty of Medicine, Jagiellonian University Medical College, Kraków, Poland.
authors: Ewa
authors: E
authors: Czarnobilska
authors: Bartosz
authors: B
authors: Jenner
authors: Jolanta
authors: J
authors: Kaszuba-Zwoinska
authors: Maria
authors: M
authors: Kapusta
authors: Krystyna
authors: K
authors: Obtułowicz
authors: Piotr
authors: P
authors: Thor
authors: Radosław
authors: R
authors: Spiewak
mesh_terms: D000293:Adolescent; D000328:Adult; D017449:Dermatitis, Allergic Contact / Q000276:immunology*; D005260:Female; D006801:Humans; D007371:Interferon-gamma / Q000096:biosynthesis* / Q000097:blood; D007376:Interleukin-2 / Q000096:biosynthesis; D015848:Interleukin-5 / Q000096:biosynthesis* / Q000097:blood; D008214:Lymphocytes / Q000187:drug effects* / Q000378:metabolism; D008875:Middle Aged; D009532:Nickel / Q000009:adverse effects*; D010328:Patch Tests
chemical_list: D007376:Interleukin-2; D015848:Interleukin-5; D009532:Nickel; D007371:Interferon-gamma