title: Bio-electrospraying whole human blood: analysing cellular viability at a molecular level.
abstract: Bio-electrosprays, pioneered in 2005, have undergone several developmental studies which have seen this technique evolve as a novel direct in vivo tissue engineering and regenerative medicinal strategy. Those studies have been a hallmark for electrosprays; however, in this communication we report our on-going developmental investigations for exploring bio-electrosprays as a potential medical device and diagnostic protocol. The studies reported here demonstrate the ability to directly jet whole human blood without affecting the genetic make-up, which has been interrogated by way of reverse transcription-polymerase chain reaction (RT-PCR) in comparison to controls (p = 0.7337). These studies demonstrate bio-electrosprays as a possible diagnostic protocol.
authors: BioPhysics Group, Department of Mechanical Engineering, University College London, UK.
authors: Napachanok
authors: N
authors: Mongkoldhumrongkul
authors: Steve
authors: S
authors: Best
authors: Emma
authors: E
authors: Aarons
authors: Suwan N
authors: SN
authors: Jayasinghe
mesh_terms: D018929:Cell Culture Techniques / Q000295:instrumentation / Q000379:methods; D002462:Cell Membrane / Q000378:metabolism; D002470:Cell Survival; D004563:Electrochemistry / Q000379:methods; D004867:Equipment Design; D015870:Gene Expression; D006801:Humans; D044085:Microfluidics / Q000295:instrumentation; D020411:Oligonucleotide Array Sequence Analysis; D012038:Regeneration; D020133:Reverse Transcriptase Polymerase Chain Reaction; D023822:Tissue Engineering / Q000379:methods*