Use of a new single multiplex PCR-based assay for direct simultaneous characterization of six Neisseria meningitidis serogroups.
We developed a new Neisseria meningitidis multiplex PCR to determine six serogroups, including X-specific primers, and to allow direct W135/Y discrimination. This assay offers a simple and low-cost method for serogrouping N. meningitidis from cerebrospinal fluid that could be useful in Africa.
Fraisier
Christophe
C
WHO Collaborating Centre, IRBA, Institut de Médecine Tropicale du Service de Santé des Armées, Marseille, France.
Stor
Richard
R
Tenebray
Bernard
B
Sanson
Yannick
Y
Nicolas
Pierre
P
D000349:Africa; D015373:Bacterial Typing Techniques / Q000379:methods; D002555:Cerebrospinal Fluid / Q000382:microbiology; D017931:DNA Primers / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D005798:Genes, Bacterial; D005838:Genotype; D006801:Humans; D008585:Meningitis, Meningococcal / Q000175:diagnosis* / Q000382:microbiology*; D009345:Neisseria meningitidis / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012703:Serotyping / Q000379:methods
D017931:DNA Primers; D004269:DNA, Bacterial