title: Altered gene profile of placenta from women with intrahepatic cholestasis of pregnancy.
abstract: OBJECTIVE
  To investigate the alterations in gene profile of placenta from pregnant women with intrahepatic cholestasis of pregnancy (ICP) and to enhance the insight of etiology and pathogenesis of ICP.
  
  
  METHODS
  Ten pregnant women diagnosed ICP were recruited and 10 healthy pregnant women served as control. Four samples were taken from each placenta and RNA was isolated. Gene expression was analyzed with microarray and real time PCR was used to validate the differentially expressed genes.
  
  
  RESULTS
  392 genes were found differentially expressed. Among these differentially expressed genes, 280 were up-regulated and 112 were down-regulated. These differentially expressed genes involved 20 categories including genes involved in transportation, cell growth, apoptosis and immune response that were putatively participated the pathogenesis of ICP.
  
  
  CONCLUSIONS
  293 differentially expressed genes of 20 categories were found in ICP placenta, suggesting the diversity of gene expression alteration and the complexity of etiology and pathogenesis of ICP.
authors: Women's Hospital, School of Medicine, Zhejiang University, Add: 2 Xueshi Road, 310006, Hangzhou, China.
authors: Jing
authors: J
authors: Wei
authors: Huimin
authors: H
authors: Wang
authors: Xiaofu
authors: X
authors: Yang
authors: Minyue
authors: M
authors: Dong
authors: Zhengping
authors: Z
authors: Wang
mesh_terms: D000328:Adult; D016022:Case-Control Studies; D002780:Cholestasis, Intrahepatic / Q000378:metabolism*; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis; D010920:Placenta / Q000378:metabolism*; D011247:Pregnancy; D011248:Pregnancy Complications / Q000378:metabolism*; D055815:Young Adult