Oocyte recovery post human follicular fluid centrifugation in modified natural cycle and achieving embryo.
This case reports a successful live birth by intracytoplasmic sperm injection (ICSI) following human follicular fluid (HFF) centrifugation for oocyte retrieval in the modified natural cycle of a poor responder patient. A 37-year-old patient presenting with a severe ovarian defect underwent a modified natural cycle with HFF centrifugation prior to ICSI. As there was only one oocyte under direct binocular observation, HFF was centrifuged and a second oocyte was collected. ICSI was performed on both oocytes. Embryo quality and outcome were not compromised by HFF centrifugation. A live birth was achieved in April 2008. In a modified natural cycle, HFF centrifugation avoided loss of oocytes, optimized the IVF treatment, and achieved the development of two embryos.
Ferrières
Alice
A
Département de Médecine et Biologie de la Reproduction, Hôpital Arnaud de Villeneuve, Université Montpellier I, UFR de Médecine, INSERM U, France.
Reyftmann
Lionel
L
Pellestor
Franck
F
Hédon
Bernard
B
Decaud
Hervé
H
Hamamah
Samir
S
D000328:Adult; D002498:Centrifugation / Q000379:methods*; D005260:Female; D015571:Follicular Fluid / Q000166:cytology*; D006801:Humans; D054315:Oocyte Retrieval / Q000379:methods*; D011247:Pregnancy; D011256:Pregnancy Outcome; D020554:Sperm Injections, Intracytoplasmic