The expression of the Goodpasture antigen-binding protein (ceramide transporter) in adult rat brain.
The Goodpasture antigen-binding protein (GPBP) plays a critical role in brain development. Knockdown of GPBP leads to loss of myelinated tracts in the central nervous system and to extensive apoptosis in the brain during early embryogenesis. GPBP was initially identified as a protein associated with the autoantigen in Goodpasture autoimmune syndrome, where it was shown to be a kinase that regulates type IV collagen organization. GPBP isoforms bind and transport ceramide from the endoplasmic reticulum to the Golgi apparatus and are therefore also known as ceramide transporters (CERT). Ceramide dysregulation is involved in autoimmunity and neurodegenerative disorders. In order to analyze the possible role of GPBP in neuroinflammation and neurodegeneration we studied the basal GPBP expression in normal rat brain. High levels of immunoreactivity were detected in neurons of the cerebral cortex, hippocampal formation, the basal ganglia, the olfactory bulb and nuclei of the thalamus, the hypothalamus and the septal area. Lower expression levels of GPBP were observed widely throughout the brain, suggesting that GPBP plays an important role in central nervous system neuron function.
Mencarelli
Chiara
C
Department of Neuroscience, School of Mental Health and Neuroscience, Maastricht University, Maastricht, The Netherlands.
Hammels
Caroline
C
Van Den Broeck
Joost
J
Losen
Mario
M
Steinbusch
Hellen
H
Revert
Francisco
F
Saus
Juan
J
Hopkins
David A
DA
De Baets
Marc H
MH
Steinbusch
Harry W
HW
Martinez-Martinez
Pilar
P
D000818:Animals; D015153:Blotting, Western; D001921:Brain / Q000033:anatomy & histology / Q000201:enzymology*; D001931:Brain Mapping; D002518:Ceramides / Q000378:metabolism*; D004027:Diencephalon / Q000033:anatomy & histology / Q000201:enzymology; D004721:Endoplasmic Reticulum / Q000378:metabolism; D006056:Golgi Apparatus / Q000378:metabolism; D007150:Immunohistochemistry; D008297:Male; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D013687:Telencephalon / Q000033:anatomy & histology / Q000201:enzymology
D002518:Ceramides; C545703:Cert1 protein, rat; D017346:Protein Serine-Threonine Kinases