title: Adducin forms a bridge between the erythrocyte membrane and its cytoskeleton and regulates membrane cohesion.
abstract: The erythrocyte membrane skeleton is the best understood cytoskeleton. Because its protein components have homologs in virtually all other cells, the membrane serves as a fundamental model of biologic membranes. Modern textbooks portray the membrane as a 2-dimensional spectrin-based membrane skeleton attached to a lipid bilayer through 2 linkages: band 3-ankyrin-beta-spectrin and glycophorin C-protein 4.1-beta-spectrin.(1-7) Although evidence supports an essential role for the first bridge in regulating membrane cohesion, rupture of the glycophorin C-protein 4.1 interaction has little effect on membrane stability.(8) We demonstrate the existence of a novel band 3-adducin-spectrin bridge that connects the spectrin/actin/protein 4.1 junctional complex to the bilayer. As rupture of this bridge leads to spontaneous membrane fragmentation, we conclude that the band 3-adducin-spectrin bridge is important to membrane stability. The required relocation of part of the band 3 population to the spectrin/actin junctional complex and its formation of a new bridge with adducin necessitates a significant revision of accepted models of the erythrocyte membrane.
authors: Department of Chemistry, Purdue University, West Lafayette, IN 47907-2084, USA.
authors: William A
authors: WA
authors: Anong
authors: Taina
authors: T
authors: Franco
authors: Haiyan
authors: H
authors: Chu
authors: Tahlia L
authors: TL
authors: Weis
authors: Emily E
authors: EE
authors: Devlin
authors: David M
authors: DM
authors: Bodine
authors: Xiuli
authors: X
authors: An
authors: Narla
authors: N
authors: Mohandas
authors: Philip S
authors: PS
authors: Low
mesh_terms: D000199:Actins / Q000378:metabolism; D019921:Biotinylation; D002148:Calmodulin-Binding Proteins / Q000378:metabolism / Q000502:physiology*; D002462:Cell Membrane / Q000378:metabolism*; D003593:Cytoplasm / Q000378:metabolism; D003599:Cytoskeleton / Q000378:metabolism*; D004910:Erythrocyte Membrane / Q000378:metabolism*; D004912:Erythrocytes / Q000378:metabolism; D005982:Glutathione Transferase / Q000378:metabolism; D006801:Humans; D008051:Lipid Bilayers / Q000378:metabolism; D008954:Models, Biological; D017434:Protein Structure, Tertiary; D011994:Recombinant Proteins / Q000737:chemistry; D020349:Surface Plasmon Resonance; D000099291:Adducins
chemical_list: D000199:Actins; D002148:Calmodulin-Binding Proteins; D005982:Glutathione Transferase; D008051:Lipid Bilayers; D011994:Recombinant Proteins; D000099291:Adducins