Potent triple helix stabilization by 5',3'-modified triplex-forming oligonucleotides.
Anthraquinone and pyrene analogues attached to the 3' and/or 5' termini of triplex-forming oligonucleotides (TFOs) by various linkers increased the stability of parallel triple helices. The modifications are simple to synthesize and can be introduced during standard solid-phase oligonucleotide synthesis. Potent triplex stability was achieved by using doubly modified TFOs, which in the most favourable cases gave an increase in melting temperature of 30 degrees C over the unmodified counterparts and maintained their selectivity for the correct target duplex. Such TFOs can produce triplexes with melting temperatures of 40 degrees C at pH 7 even though they do not contain any triplex-stabilizing base analogues. These studies have implications for the design of triplex-forming oligonucleotides for use in biology and nanotechnology.
Ben Gaied
Nouha
N
School of Chemistry, University of Southampton, Highfield, SO17 1BJ, Southampton, UK.
Zhao
Zhengyun
Z
Gerrard
Simon R
SR
Fox
Keith R
KR
Brown
Tom
T
D000588:Amines / Q000138:chemical synthesis / Q000737:chemistry; D000880:Anthraquinones / Q000138:chemical synthesis / Q000737:chemistry*; D001483:Base Sequence; D004247:DNA / Q000737:chemistry*; D009690:Nucleic Acid Conformation; D009841:Oligonucleotides / Q000737:chemistry*; D011721:Pyrenes / Q000138:chemical synthesis / Q000737:chemistry*; D044366:Transition Temperature; D014466:Ultraviolet Rays
D000588:Amines; D000880:Anthraquinones; D009841:Oligonucleotides; D011721:Pyrenes; C051802:triplex DNA; D004247:DNA