title: Polyclonal IgE induces mast cell survival and cytokine production.
abstract: BACKGROUND
  Ag-dependent activation of IgE-bearing mast cells is a critical first step in immediate hypersensitivity and other allergic responses. Recent studies have revealed Ag-independent effects of monoclonal mouse IgE molecules on mast cell survival and activation. However, no studies have been performed on the effects of polyclonal IgE molecules. Here, we tested whether polyclonal mouse and human IgE molecules affect survival and cytokine production in mast cells.
  
  
  METHODS
  Mast cells were cultured in the presence of polyclonal mouse and human IgE molecules, and cell survival and cytokine production were analyzed.
  
  
  RESULTS
  Polyclonal mouse IgE molecules in sera from mice with atopic dermatitis-like allergic skin inflammation, enhanced survival and cytokine production in mast cell cultures. Similar to the effects of monoclonal IgE, the polyclonal IgE effects were mediated by the high-affinity IgE receptor, FcepsilonRI. Human polyclonal IgE molecules present in sera from atopic dermatitis patients were also capable of activating mast cells, and inducing IL-8 production in human cord blood-derived mast cells.
  
  
  CONCLUSIONS
  These results imply that polyclonal IgE in atopic dermatitis and other atopic conditions might modulate mast cell number and function, thus amplifying the allergic response.
authors: Division of Cell Biology, La Jolla Institute for Allergy and Immunology, CA, USA.
authors: Jun-ichi
authors: J
authors: Kashiwakura
authors: Yuko
authors: Y
authors: Kawakami
authors: Keisuke
authors: K
authors: Yuki
authors: Dirk M
authors: DM
authors: Zajonc
authors: Shunji
authors: S
authors: Hasegawa
authors: Yoshiaki
authors: Y
authors: Tomimori
authors: Benjamin
authors: B
authors: Caplan
authors: Hirohisa
authors: H
authors: Saito
authors: Masutaka
authors: M
authors: Furue
authors: Hans C
authors: HC
authors: Oettgen
authors: Yoshimichi
authors: Y
authors: Okayama
authors: Toshiaki
authors: T
authors: Kawakami
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells; D002470:Cell Survival / Q000276:immunology; D002478:Cells, Cultured; D016207:Cytokines / Q000096:biosynthesis*; D003876:Dermatitis, Atopic / Q000276:immunology*; D005312:Fetal Blood / Q000276:immunology; D006801:Humans; D007073:Immunoglobulin E / Q000276:immunology*; D008407:Mast Cells / Q000276:immunology*; D051379:Mice; D008810:Mice, Inbred C57BL; D017455:Receptors, IgE / Q000235:genetics / Q000276:immunology
chemical_list: D016207:Cytokines; D017455:Receptors, IgE; D007073:Immunoglobulin E