[Isolation and identification of a 4-aminobenzenesulphonate biodegrading strain and its degradation characteristics].
A 4-aminobenzenesulphonate-degrading strain W1 was isolated from the activated sludge of Dalian municipal wastewater treatment plant. Strain W1 was able to utilize 4-ABS as sole carbon and energy source under aerobic condition. It was identified as Pannonibacter sp. according to its morphological, physiological and biochemical characteristics and the analysis of its 16S rDNA gene. The optimum conditions for 4-ABS biodegradation in the shaking flasks were 10% inoculum, 30 degrees C, pH 7.0 and were rotated at speed 150 r/min, respectively. Strain W1 could keep high degrading ability even in the presence of extra carbon source. Release of ammonia and sulfate were around 77.6% and 91.5% of theoretic concentration according to 4-ABS degradation, for 4-ABS could serve as the nitrogen and sulfur source. Strain W1 showed efficient biodegradability even at 2,500 mg/L, and 90% 4-ABS removal was achieved in 32 h. The 84.4% TOC removal was achieved after 94.7% consumption of 4-ABS, and no aromatic intermediates were detected, referring the complete mineralization of 4-ABS by strain W1.
Wang
Yan-Qing
YQ
Key Laboratory of Industrial Ecology and Environmental Engineering, Ministry of Education, School of Environmental and Biological Science and Technology, Dalian University of Technology, Dalian 116023, China. aben_001@126.com
Zhang
Jin-Song
JS
Zhou
Ji-Ti
JT
Wang
Jing
J
D001673:Biodegradation, Environmental; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D041902:Rhodobacteraceae / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*; D012722:Sewage / Q000382:microbiology*; D013425:Sulfanilic Acids / Q000378:metabolism*; D014865:Waste Disposal, Fluid / Q000379:methods*; D018508:Water Purification / Q000379:methods
D012336:RNA, Ribosomal, 16S; D012722:Sewage; D013425:Sulfanilic Acids; C100016:4-sulfanilic acid