Proliferative defects in dyskeratosis congenita skin keratinocytes are corrected by expression of the telomerase reverse transcriptase, TERT, or by activation of endogenous telomerase through expression of papillomavirus E6/E7 or the telomerase RNA component, TERC.
Dyskeratosis congenita (DC) is characterized by the triad of reticulate skin pigmentation, nail dystrophy and leukoplakia. Epidermal atrophy, hair growth defects, bone marrow failure and increased risk of cancer are also common in DC patients. DC is caused by mutations in genes encoding for telomerase complex factors. Although there is an association of epidermal abnormalities with DC, epidermal cells from DC donors have not been previously characterized. We have isolated skin keratinocytes from affected members of a family with an autosomal dominant form of DC that is caused by a mutation in the RNA component of telomerase, TERC. Here, we demonstrate that, similar to DC fibroblasts from these donors, DC keratinocytes have short telomeres and a short lifespan. DC keratinocytes also exhibited impaired colony forming efficiency (CFE) and migration capacity. Exogenous expression of the reverse transcriptase (RT) component of telomerase, TERT, activated telomerase levels to half that of TERT expressing normal cells and maintained telomeres at a short length with concomitant extension of lifespan. Unlike fibroblasts, transduction of human papillomavirus type 16 E6/E7 genes into DC keratinocytes activated telomerase to half that of E6/E7 expressing normal cells, and robust proliferation was observed. While expression of TERC has no measurable effect on telomerase in fibroblasts, expression of TERC in keratinocytes upregulated telomerase activity and, rarely, allowed rescue of proliferative defects. Our results point to important differences between DC fibroblasts and keratinocytes and show, for the first time, that expression of TERC can increase the lifespan of primary human epithelial cells.
Gourronc
Francoise A
FA
Department of Microbiology, University of Iowa, Iowa City, IA, USA.
Robertson
mckaylee M
mM
Herrig
Annie K
AK
Lansdorp
Peter M
PM
Goldman
Frederick D
FD
Klingelhutz
Aloysius J
AJ
D000328:Adult; D001483:Base Sequence; D049109:Cell Proliferation; D003114:Colony-Forming Units Assay; D017931:DNA Primers / Q000235:genetics; D019871:Dyskeratosis Congenita / Q000201:enzymology* / Q000235:genetics / Q000473:pathology*; D004789:Enzyme Activation; D005260:Female; D005347:Fibroblasts / Q000201:enzymology / Q000473:pathology; D015870:Gene Expression; D006801:Humans; D066298:In Vitro Techniques; D015603:Keratinocytes / Q000201:enzymology* / Q000473:pathology*; D008297:Male; D009154:Mutation; D009856:Oncogene Proteins, Viral / Q000235:genetics; D050725:Papillomavirus E7 Proteins / Q000235:genetics; D012313:RNA / Q000235:genetics* / Q000378:metabolism; D012097:Repressor Proteins / Q000235:genetics; D019098:Telomerase / Q000235:genetics* / Q000378:metabolism*; D016615:Telomere / Q000235:genetics / Q000473:pathology; D014161:Transduction, Genetic; D015854:Up-Regulation; D055815:Young Adult
D017931:DNA Primers; C068651:E6 protein, Human papillomavirus type 16; D009856:Oncogene Proteins, Viral; D050725:Papillomavirus E7 Proteins; D012097:Repressor Proteins; C059731:oncogene protein E7, Human papillomavirus type 16; C422952:telomerase RNA; D012313:RNA; C509186:TERT protein, human; D019098:Telomerase