Viability of human fibroblasts in coconut water as a storage medium.
OBJECTIVE
To evaluate the effectiveness of a new storage medium for avulsed teeth, coconut water, in maintaining the viability of human fibroblasts.


METHODS
Cell viability after different time periods was evaluated in the following storage media: coconut water, coconut water with sodium bicarbonate, milk, saline and still mineral water. Human fibroblasts were seeded in Eagle's minimal essential medium (EMEM) supplemented with 7.5% foetal calf serum. After trypsinisation, 100 microL of culture medium containing approximately 10(4) cells mL(-1) were collected and pipetted into the wells of 96-well plates, which were incubated overnight in 5% CO(2) and 95% air mixture at 37 degrees C. EMEM was then replaced by the storage media and the plates were incubated at 37 degrees C for 1, 2 and 4 h. Cell viability was determined using the neutral red assay. The proportions of viable cells after exposure to the storage media were analysed statistically by anova and the least significant difference (LSD) test (alpha = 5%).


RESULTS
Milk had the greatest capacity to maintain cell viability (P < 0.05), followed by coconut water with sodium bicarbonate and saline. Coconut water was significantly worse at maintaining cell viability compared to milk, coconut water with sodium bicarbonate and saline. The smallest number of viable cells was observed for mineral water (P < 0.05).


CONCLUSIONS
Coconut water was worse than milk in maintaining human fibroblast cell viability.
Moreira-Neto
J J S
JJ
Department of Dental Clinics, School of Pharmacy, Dentistry and Nursing, Federal University of Ceará, CE, Brazil.
Gondim
J O
JO
Raddi
M S G
MS
Pansani
C A
CA
D000704:Analysis of Variance; D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D003059:Cocos; D005347:Fibroblasts / Q000166:cytology* / Q000187:drug effects; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007552:Isotonic Solutions; D008892:Milk; D019188:Organ Preservation Solutions; D009994:Osmolar Concentration; D053147:Plant Exudates / Q000627:therapeutic use; D028321:Plant Preparations / Q000627:therapeutic use*; D014021:Tissue Preservation / Q000379:methods*; D014084:Tooth Avulsion
D007552:Isotonic Solutions; D019188:Organ Preservation Solutions; D053147:Plant Exudates; D028321:Plant Preparations