The use of additives as the stimulator on mycelial biomass and exopolysaccharide productions in submerged culture of Grifola umbellata.
In this study, various additives including organic acids, alcohols, vegetable oils, surfactants and polymers were added in the cultural medium to investigate their stimulatory effects on Grifola umbellate mycelia growth and exopolysaccharide (EPS) production. It was found that the commonly used stimulatory additives, effective in other mushrooms' cultures, exhibited negative results in Grifola umbellata submerged culture. In contrast, the polymer additive, polyethylene glycol (PEG), displayed an effective stimulatory effect on both biomass and EPS productions. With the addition of PEG8 (molecular weight: 8,000 Da), the mycelial biomass production at day 12 was increased from 4.69 to 6.30 g/L, accounting for a 34% increase. Meanwhile, the EPS production was enhanced from 0.478 to 0.767 g/L, accounting for 60% increase.
Chen
Hua-Bing
HB
Department of Chemical Engineering, National Chung Hsing University, 250, Kuo Kuang Road, Taichung 40227, Taiwan, ROC.
Huang
Hung-Chang
HC
Chen
Chih-I
CI
I
Yet-Pole
YP
Liu
Yung-Chuan
YC
D000363:Agaricales; D000438:Alcohols / Q000737:chemistry; D000818:Animals; D018533:Biomass; D001709:Biotechnology / Q000379:methods*; D005285:Fermentation; D005947:Glucose / Q000737:chemistry; D048691:Grifola / Q000378:metabolism*; D008970:Molecular Weight; D025282:Mycelium / Q000378:metabolism*; D011092:Polyethylene Glycols / Q000737:chemistry; D011134:Polysaccharides / Q000737:chemistry*; D013501:Surface-Active Agents / Q000737:chemistry; D013997:Time Factors
D000438:Alcohols; D011134:Polysaccharides; D013501:Surface-Active Agents; D011092:Polyethylene Glycols; D005947:Glucose