Continuous hydrogen and butyric acid fermentation by immobilized Clostridium tyrobutyricum ATCC 25755: effects of the glucose concentration and hydraulic retention time.
The effects of the hydraulic retention time (HRT=8, 10, 12 or 16.7 h) and glucose concentration (30, 40 or 50 g/L) on the production of hydrogen and butyrate by an immobilized Clostridium tyrobutyricum culture, grown under continuous culturing conditions, were evaluated. With 30 g/L glucose, the higher HRTs tested led to greater butyrate concentrations in the culture, i.e., 9.3 g/L versus 12.9 g/L with HRTs of 8 h and 16.7 h, respectively. In contrast, higher biogas and hydrogen production rates were generally seen when the HRT was lower. Experiments with different glucose concentrations saw a significant amount of glucose washed out when 50 g/L was used, the highest being 22.7 g/L when the HRT was 16.7 h. This study found the best conditions for the continuous production of hydrogen and butyric acid by C. tyrobutyricum to be with an HRT of 12 h and a glucose concentration of 50 g/L, respectively.
Mitchell
Robert J
RJ
Division of Nano-Bio and Chemical Engineering, Ulsan National Institute of Science and Technology, San 194 Banyeon-Ri, Eonyang-Eup, Ulju-Gun, Ulsan 689-805, Republic of Korea.
Kim
Ji-Seong
JS
Jeon
Byung-Seung
BS
Sang
Byoung-In
BI
D019149:Bioreactors; D020148:Butyric Acid / Q000378:metabolism*; D018914:Cells, Immobilized / Q000166:cytology / Q000187:drug effects; D048014:Clostridium tyrobutyricum / Q000166:cytology* / Q000187:drug effects*; D005285:Fermentation / Q000187:drug effects*; D005947:Glucose / Q000494:pharmacology*; D006859:Hydrogen / Q000378:metabolism*; D013997:Time Factors
D020148:Butyric Acid; D006859:Hydrogen; D005947:Glucose