title: Blocking of Pseudomonas aeruginosa and Ralstonia solanacearum Lectins by plant and microbial branched polysaccharides used as food additives.
abstract: Pseudomonas aeruginosa antibiotic resistance prompted the search for glycodecoys that would block its lectin-dependent adhesion to human cells. We have used the lectins of this pathogen, PA-IL (galactophilic LecA) and PA-IIL (fucophilic LecB), and two additional pathogenic bacterial lectins, CV-IIL (fucophilic, of Chromobacterium violaceum) and RS-IIL (mannophilic, of Ralstonia solanacearum), for assaying the pathogenic lectin-blocking abilities of some plant and microbial polysaccharidic food additives, adding the mannophilic plant lectin Con A as a reference. Locust-bean and guar galactomannans and acacia gum very strongly inhibited PA-IL. The other lectins, excluding CV-IIL, were very strongly inhibited by yeast mannan. Xanthan and inulin were weak inhibitors. The differential blocking of these lectins by galactosylated branches of plant polysaccharides and by mannan matched their inhibition by avian egg whites, human milk, and royal jelly (protecting animal embryos and neonates from infections). The nondigestability and nontoxicity of the food additives are advantageous for curing gastrointestinal and external infections.
authors: The Mina & Everard Goodman Faculty of Life Sciences, Bar-Ilan University, Ramat-Gan 52900, Israel.
authors: Keren D
authors: KD
authors: Zinger-Yosovich
authors: Nechama
authors: N
authors: Gilboa-Garber
mesh_terms: D000818:Animals; D001422:Bacterial Adhesion / Q000187:drug effects; D001426:Bacterial Proteins / Q000037:antagonists & inhibitors* / Q000378:metabolism; D002417:Cattle; D010856:Columbidae; D004529:Egg White / Q000737:chemistry; D005227:Fatty Acids / Q000737:chemistry; D005260:Female; D005503:Food Additives / Q000737:chemistry / Q000494:pharmacology*; D006801:Humans; D037102:Lectins / Q000037:antagonists & inhibitors* / Q000378:metabolism; D008892:Milk / Q000737:chemistry; D053149:Plant Gums / Q000737:chemistry / Q000494:pharmacology*; D011135:Polysaccharides, Bacterial / Q000737:chemistry / Q000494:pharmacology*; D011485:Protein Binding; D011550:Pseudomonas aeruginosa / Q000187:drug effects* / Q000502:physiology; D011784:Quail; D043368:Ralstonia solanacearum / Q000187:drug effects* / Q000502:physiology; D000099219:Royal Jelly
chemical_list: D001426:Bacterial Proteins; D005227:Fatty Acids; D005503:Food Additives; D037102:Lectins; D053149:Plant Gums; D011135:Polysaccharides, Bacterial; D000099219:Royal Jelly