[Experimental models in oncology: contribution of cell culture on understanding the biology of cancer].
In the beginning of the 20th century, tissue culture was started with the aim of studying the behaviour of animal cells in normal and stress conditions. The cell study at molecular level depends on their capacity of growing and how they can be manipulated in laboratory. In vitro cell culture allows us the possibility of studying biological key processes, such as growth, differentiation and cell death, and also to do genetic manipulations essential to the knowledge of structure and genes function. Human stem cells culture provides strategies to circumvent other models' deficiencies. It seems that cancer stem cells remain quiescent until activation by appropriated micro-environmental stimulation. Several studies reveal that different cancer types could be due to stem cell malignant transformations. Removal of these cells is essential to the development of more effective cancer therapies for advanced disease. On the other hand, dendritic cells modified in culture may be used as a therapeutic vaccine in order to induce tumour withdraw.
Cruz
Mariana
M
Faculdade de Medicina da Universidade de Coimbra, Coimbra, Portugal.
Enes
Margarida
M
Pereira
Marta
M
Dourado
Marília
M
Sarmento Ribeiro
Ana Bela
AB
D018929:Cell Culture Techniques / Q000379:methods; D003713:Dendritic Cells; D006801:Humans; D008175:Lung Neoplasms / Q000473:pathology; D009369:Neoplasms / Q000473:pathology*; D014407:Tumor Cells, Cultured