title: Highly cross-linked polymeric capillary monoliths for the separation of low, medium, and high molecular weight analytes.
abstract: Highly rigid capillary monoliths with low swelling propensity were prepared within the confines of 200 microm ID fused silica capillaries via thermally induced free radical polymerization of tetrakis(4-vinylbenzyl)silane (TVBS) in the presence of 1-dodecanol and toluene. alpha,alpha'-Azobisisobutyronitrile (AIBN) was used as initiator. The resulting monoliths were optimized for the separation of low, medium, and high molecular weight analytes. The microstructure and porosity of the monoliths prepared were studied by SEM and inverse size-exclusion chromatography (ISEC). The porosity of the monolithic supports was tuned by varying the amount of initiator (i. e. AIBN) between 0.5 and 2 wt%. All monoliths were tested for a series of low molecular weight compounds including alkylbenzenes, amines, carboxylic acids, phenols, carbonyl compounds, and beta-blockers, as well as for the separation of medium molecular weight analytes such as peptides and high-molecular weight analytes such as proteins. Due to the microporous structure, the novel monoliths displayed high efficiency and performance particularly in the separation of low molecular weight analytes. Relevant chromatographic parameters including permeability, swelling propensity, and height equivalents to theoretical plates were determined.
authors: Leibniz-Institut für Oberflächenmodifizierung, Leipzig, Germany.
authors: Said H
authors: SH
authors: Lubbad
authors: Michael R
authors: MR
authors: Buchmeiser
mesh_terms: D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation* / Q000379:methods; D003432:Cross-Linking Reagents / Q000737:chemistry; D008855:Microscopy, Electron, Scanning; D015394:Molecular Structure; D008970:Molecular Weight; D009696:Nucleic Acids / Q000032:analysis; D009930:Organic Chemicals / Q000032:analysis; D010455:Peptides / Q000032:analysis; D010539:Permeability; D004364:Pharmaceutical Preparations / Q000032:analysis; D011108:Polymers / Q000737:chemistry*; D016062:Porosity; D011506:Proteins / Q000032:analysis; D015203:Reproducibility of Results; D012821:Silanes / Q000737:chemistry; D012822:Silicon Dioxide / Q000737:chemistry
chemical_list: D003432:Cross-Linking Reagents; D009696:Nucleic Acids; D009930:Organic Chemicals; D010455:Peptides; D004364:Pharmaceutical Preparations; D011108:Polymers; D011506:Proteins; D012821:Silanes; D012822:Silicon Dioxide