CHOP 5'UTR-c.279T>C and +nt30C>T variants are not associated with overweight condition or with tumors/cancer in Italians - a case-control study.
BACKGROUND
Type 2 diabetes (T2D) is associated with obesity and has been shown recently to be associated with tumors/cancer. HNF1-beta and JAZF1 genes are associated with T2D and prostate cancer. We have previously shown that CHOP 5'UTR-c.279T>C and +nt30C>T haplotype variants contribute to T2D. CHOP deficiency causes obesity in mice, thus CHOP gene variants may contribute to human obesity. Furthermore, CHOP mediates apoptosis and is implicated in cancer pathogenesis. Hence, we aimed at identifying any potential association of CHOP 5'UTR-c.279T>C and +nt30C>T genotypes and corresponding haplotypes with overweight condition/pre-obesity and tumors/cancer in an Italian dataset.


METHODS
We recruited from Italy 45 overweight subjects (body mass index (BMI) >or= 25) and 44 control subjects (BMI < 25) as well as 54 cases with at least one cancer or at least one tumor and 43 control subjects without tumors/cancer from the general population. We excluded allelic departure from Hardy-Weinberg equilibrium in cases and control subjects, separately.


RESULTS
We assessed the power to detect risk odds ratios by association tests in our datasets. We tested the hypothesis of association of CHOP 5'UTR-c.279T>C and +nt30C>T genotypes and haplotypes with tumors/cancer and, separately, with overweight condition. Both associations were not significant.


CONCLUSIONS
From our study, we may conclude that CHOP 5'UTR-c.279T>C and +nt30C>T genotypes and corresponding haplotypes are not associated with tumors/cancer and pre-obesity. However, more studies are warranted to establish the role of CHOP variants in tumor/cancer predisposition and in overweight condition.
Meenakshisundaram
Ramachandran
R
Department of Medicine and Cellular & Molecular Physiology and Biostatistics, Penn State Milton S, Hershey Medical Center, Hershey, Pennsylvania, USA. rmsundar_chandran@yahoo.co.in
Piumelli
Nunzia
N
Pierpaoli
Laura
L
Gragnoli
Claudia
C
D020121:5' Untranslated Regions / Q000235:genetics*; D016022:Case-Control Studies; D003924:Diabetes Mellitus, Type 2 / Q000453:epidemiology / Q000235:genetics; D014644:Genetic Variation; D006239:Haplotypes; D006801:Humans; D007558:Italy / Q000453:epidemiology; D009369:Neoplasms / Q000453:epidemiology / Q000235:genetics*; D009765:Obesity / Q000453:epidemiology / Q000235:genetics*; D050177:Overweight / Q000453:epidemiology / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide; D051742:Transcription Factor CHOP / Q000235:genetics*
D020121:5' Untranslated Regions; C497249:DDIT3 protein, human; D051742:Transcription Factor CHOP