Synergistic production of interleukin-23  by dendritic cells derived from cord  blood in response to costimulation  with LPS and IL-12.
This study was performed to provide insight for the optimization and regulation of immune homeostasis, which should be taken into account in the development of cell therapy using DCs and/or cytokine. Human CBDCs costimulated with LPS and IL-12 were examined for cytokine expression compared with ABDCs. Our results showed that costimulation with IL-12 and LPS in CBDCs resulted in increased expression of IL-23. Concomitantly, the phosphorylation of ERKs and p38 MAPK was increased, suggesting that these kinases are important signaling components for IL-23 induction in CBDC costimulated with LPS and IL-12. Furthermore, production of IL-23 in CBDC costimulated with LPS and IL-12 caused CD4(+)CD45RO(+) memory cells to increase IFN-gamma production. Taken together, CBDCs, costimulated with LPS and IL-12, show a synergistic increase in IL-23 production via enhanced phosphorylation of ERK1/2 and p38 MAPK and consequently, an induction of IFN-gamma production in the memory cells.
Jang
Mi Seon
MS
Protein Engineering and Comparative Immunology, Department of Agricultural Biotechnology and Research Institute for Agriculture and Life Sciences, Seoul National University, Seoul 151-921, Republic of Korea.
Son
Young Min
YM
Kim
Gi Rak
GR
Lee
Yeo Jin
YJ
Lee
Woon Kyu
WK
Cha
Seok Ho
SH
Han
Seung Hyun
SH
Yun
Cheol-Heui
CH
D003713:Dendritic Cells / Q000378:metabolism*; D004357:Drug Synergism; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism; D005312:Fetal Blood / Q000378:metabolism*; D006801:Humans; D007156:Immunologic Memory; D007371:Interferon-gamma / Q000096:biosynthesis; D018664:Interleukin-12 / Q000378:metabolism*; D053759:Interleukin-23 / Q000096:biosynthesis*; D008070:Lipopolysaccharides / Q000494:pharmacology*; D010766:Phosphorylation; D013601:T-Lymphocytes / Q000378:metabolism; D048051:p38 Mitogen-Activated Protein Kinases / Q000378:metabolism
D053759:Interleukin-23; D008070:Lipopolysaccharides; D018664:Interleukin-12; D007371:Interferon-gamma; D048049:Extracellular Signal-Regulated MAP Kinases; D048051:p38 Mitogen-Activated Protein Kinases