title: [Protective effect of polysaccharides from sea cucumber on glu-induced neurotoxicity in PC12 cells].
abstract: OBJECTIVE
  To investigate the protective effect of polysaccharides from sea cucumber (HS) on Glu-induced neurotoxicity in PC12 cells.
  
  
  METHODS
  The toxicity model was established by treating PC12 cells with Glu in vitro. Cell viability was assayed with MTT method. The vitality of antioxidant enzymes (SOD) and the level of malondialdehyde (MDA) and lactic dehydrogenase (LDH) were evaluated by chromatometry.
  
  
  RESULTS
  The vitality of SOD increased and the levels of MDA and LDH significantly decreased in PC12 cells of HS group compared with the model group. HS in different concentration showed protective effects as it increased the vitality of SOD and decreased the content of MDA and LDH in a dose-dependent manner.
  
  
  CONCLUSIONS
  Polysaccharide from sea cucumber can protect PC12 cells from the injury induced by Glu.
authors: Weihai International Biotechnology R & D Center of Shandong University, Weihai 264209, China.
authors: Hong-Xia
authors: HX
authors: Diao
authors: Shu-Liang
authors: SL
authors: Song
authors: Hao
authors: H
authors: Liang
authors: Yun-Shan
authors: YS
authors: Wang
authors: Wei-Li
authors: WL
authors: Wang
authors: Ai-Guo
authors: AG
authors: Ji
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D002470:Cell Survival / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D005971:Glutamates / Q000633:toxicity; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008315:Malondialdehyde / Q000378:metabolism; D018696:Neuroprotective Agents / Q000494:pharmacology*; D018384:Oxidative Stress / Q000187:drug effects; D016716:PC12 Cells; D011134:Polysaccharides / Q000494:pharmacology*; D051381:Rats; D012616:Sea Cucumbers / Q000737:chemistry*; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D000975:Antioxidants; D005971:Glutamates; D018696:Neuroprotective Agents; D011134:Polysaccharides; D008315:Malondialdehyde; D007770:L-Lactate Dehydrogenase; D013482:Superoxide Dismutase