Flower and root oils of the tunisian Daucus carota L. ssp. maritimus (Apiaceae): integrated analyses by GC, GC/MS, and 13C-NMR spectroscopy, and in vitro antibacterial activity.
The essential oils from flowers and roots of Daucus carota L. ssp. maritimus were obtained by hydrodistillation and analyzed by combination of GC, GC/MS, and (13)C-NMR. The chemical composition of the flower and root oils of this subspecies is reported here for the first time. Thirty-two and six compounds were identified in flower and root oils, respectively. A remarkable difference was found between the constituent percentages of the two organs. The chemical composition of the essential oil from flowers was characterized by a high proportion of monoterpene hydrocarbons (68.4%) and belonged to the sabinene (4; 51.6%) chemotype. The oxygenated monoterpenes represented the second major fraction of the same oil among which terpinen-4-ol (11.0%) was the predominant compound. Root oil exhibited a surprisingly different composition. Phenolic derivatives (76.3%), myristicin (31; 29.7%), and dillapiole (32; 46.6%) constituted the main fraction. The antibacterial effect resulted in the inhibition of a series of common human pathogenic bacteria, and of some clinically and environmentally isolated strains with significant MIC and MBC values.
Jabrane
Aymen
A
Laboratoire de Chimie des Substances Naturelles et de Synthèse Organique (99/UR/12-26), Faculté des Sciences de Monastir, Université de Monastir, Avenue de l'Environnement, Monastir, 5000, Tunisie.
Jannet
Hichem Ben
HB
Harzallah-Skhiri
Féthia
F
Mastouri
Maha
M
Casanova
Joseph
J
Mighri
Zine
Z
D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000494:pharmacology; D002247:Carbon Isotopes / Q000737:chemistry; D002849:Chromatography, Gas; D018552:Daucus carota / Q000737:chemistry*; D035264:Flowers / Q000737:chemistry*; D008401:Gas Chromatography-Mass Spectrometry; D009682:Magnetic Resonance Spectroscopy; D008826:Microbial Sensitivity Tests; D009822:Oils, Volatile / Q000737:chemistry* / Q000494:pharmacology; D018517:Plant Roots / Q000737:chemistry*
D000900:Anti-Bacterial Agents; D002247:Carbon Isotopes; D009822:Oils, Volatile