Evaluation of target specificity of antibacterial agents using Staphylococcus aureus ddlA mutants and D-cycloserine in a silkworm infection model.
The availability of a silkworm larva infection model to evaluate the therapeutic effectiveness of antibiotics was examined. The 50% effective doses (ED50) of D-cycloserine against the Staphylococcus aureus ddlA mutant-mediated killing of larvae were remarkably lower than those against the parental strain-mediated killing of larvae. Changes in MICs and ED50 of other antibiotics were negligible, suggesting that these alterations are d-cycloserine selective. Therefore, this model is useful for selecting desired compounds based on their therapeutic effectiveness during antibiotic development.
Kurokawa
Kenji
K
Laboratory of Microbiology, Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan. kurokawa@mol.f.u-tokyo.ac.jp
Hamamoto
Hiroshi
H
Matsuo
Miki
M
Nishida
Satoshi
S
Yamane
Noriko
N
Lee
Bok Luel
BL
Murakami
Kazuhisa
K
Maki
Hideki
H
Sekimizu
Kazuhisa
K
D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D012831:Bombyx / Q000382:microbiology*; D002511:Cephalosporins / Q000494:pharmacology; D002701:Chloramphenicol / Q000494:pharmacology; D003523:Cycloserine / Q000494:pharmacology*; D020128:Inhibitory Concentration 50; D007814:Larva / Q000276:immunology; D008826:Microbial Sensitivity Tests; D013211:Staphylococcus aureus / Q000187:drug effects*; D013752:Tetracycline / Q000494:pharmacology; D014640:Vancomycin / Q000494:pharmacology
D000900:Anti-Bacterial Agents; D002511:Cephalosporins; D002701:Chloramphenicol; D014640:Vancomycin; D003523:Cycloserine; D013752:Tetracycline; C045693:flomoxef