Levels of glutathione and some biogenic amines in the human brain putamen after traumatic death.
Mexico City is among the world's largest metropolitan city centers and one of the most difficult and challenging cities in which to drive a motor vehicle. During peak transit hours and maximum congestion, numerous accidents occur, many of them fatal. The aim of the study presented here was to analyze the levels of select indicators against oxidative stress and levels of biogenic amines as a consequence of accident or altercation and fear deaths. Eighteen cases were studied (sixteen males, two females). Subjects ranged from twelve to eighty-one years of age. Nine of the deaths studied were the result of motor vehicle or subway accidents. Eight of the eighteen deaths were the result of a violent altercation, while one of the deaths resulted from a drug overdose and cardiac arrest. Biopsies of cadaver putamen were homogenized and analyzed for Tryptophan (Trp), 5-hydroxyindole acetic acid (5-HIAA), Dopamine (DA), and Glutathione (GSH) levels by fluorometric methods. Trp, 5-HIAA, DA, and GSH levels showed an increase in the subjects who's death was caused by violent altercation combined with fear, while DA levels showed significant differences in all accident groups. This data suggest that biogenic amines in cadaver putamen tissue, such as DA, can be telling biochemical markers, indicative of altercation and fear deaths.
Calderón-Guzmán
David
D
Laboratorio de Neuroquímica, Instituto Nacional de Pediatría (INP), México.
Osnaya-Brizuela
Norma
N
García-Alvarez
Raquel
R
Hernández García
Ernestina
E
Guillé Pérez
Adrián
A
Juárez Olguín
Hugo
H
D000059:Accidents; D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001344:Autopsy; D001679:Biogenic Amines / Q000378:metabolism*; D015415:Biomarkers / Q000032:analysis; D002648:Child; D005239:Fear; D005260:Female; D005978:Glutathione / Q000378:metabolism*; D006801:Humans; D008297:Male; D008875:Middle Aged; D011699:Putamen / Q000378:metabolism*; D014754:Violence; D014947:Wounds and Injuries / Q000378:metabolism*
D001679:Biogenic Amines; D015415:Biomarkers; D005978:Glutathione