[Research on an improved Lowry method to determine content of protein in Sabin IPV].
OBJECTIVE
To establish a method for the content determination of protein in Sabin IPV.


METHODS
Using lowry method combined with being precipitated by trichloroacetic acid to determine the content of protein in Sabin IPV. Changing different conditions to optimize the experiment to establish a improved lowry method. And the sample recovery test was also conducted.


RESULTS
The method can exclude the interference of free aminoacid, phenols and some other additives. The calibration curve was in good linearity of protein within the range of 2.5 microg/ml-40 Microg/ml, r = 0.9998. Under the best conditions, the mean recovery was 95.32%, the CV in a batch and between batches were both < 10%.


CONCLUSIONS
The method can be used to determine the micro content of protein in vaccines.
Cai
Wei
W
Institute of Medical Biology, Chinese Academy of Medical Sciences, Pekin Union Medical College, Kunming 650118, China.
Sun
Ming-bo
MB
Chen
Hong-bo
HB
Hong
Chao
C
Song
Xiao-yan
XY
Yu
Lei
L
Jiang
Shu-de
SD
Liao
Guo-yang
GY
Yang
Jing-si
JS
D000596:Amino Acids / Q000378:metabolism; D002138:Calibration; D002623:Chemistry Techniques, Analytical / Q000379:methods*; D010636:Phenols / Q000737:chemistry; D011055:Poliovirus Vaccine, Oral / Q000737:chemistry*; D011506:Proteins / Q000032:analysis*; D014238:Trichloroacetic Acid / Q000737:chemistry
D000596:Amino Acids; D010636:Phenols; D011055:Poliovirus Vaccine, Oral; D011506:Proteins; D014238:Trichloroacetic Acid