Influence of ovariectomy on bone turnover and trabecular bone mass in mature cynomolgus monkeys.
OBJECTIVE
To examine the influence of ovariectomy (OVX) on bone turnover and trabecular bone mass at the 3 clinically important skeletal sites in mature cynomolgus monkeys.


METHODS
Six female cynomolgus monkeys, aged 17-21 years, were randomized into 2 groups by the stratified weight: the OVX and sham-operation groups (n = 3 in each group). The experimental period was 16 months. Lumbar bone mineral density (BMD) in vivo and serum and urinary bone turnover markers were longitudinally measured, and peripheral quantitative computed tomographic and bone histomorphometric analyses were performed on trabecular bone of the lumbar vertebra, femoral neck, and distal radius at the end of the experiment.


RESULTS
OVX induced in a reduction in lumbar BMD compared with the sham controls and the baseline, as a result of increased serum levels of bone-specific alkaline phosphatase and urinary levels of cross-lined N- and C-terminal telopeptides of type I collagen. Furthermore, OVX induced reductions in trabecular volumetric BMD and trabecular bone mass compared with the sham controls, with increased bone formation rate at the lumbar vertebra, femoral neck, and distal radius.


CONCLUSIONS
The results indicated that OVX in mature cynomolgus monkeys (17-21 years of age) increased bone turnover and induced trabecular bone loss at the three skeletal sites compared with the sham controls. Thus, mature cynomolgus monkeys could be utilized for preclinical studies to examine the effects of interventions on bone turnover and trabecular bone mass at the 3 clinically important skeletal sites.
Iwamoto
Jun
J
Institute for Integrated Sports Medicine, Keio University School of Medicine, 35 Shinanomachi, Shinjuku-ku, Tokyo, Japan. jiwamoto@sc.itc.keio.ac.jp
Seki
Azusa
A
Matsuura
Masao
M
Sato
Yoshihiro
Y
Takeda
Tsuyoshi
T
Matsumoto
Hideo
H
Yeh
James K
JK
D000469:Alkaline Phosphatase / Q000097:blood; D000818:Animals; D015519:Bone Density; D024042:Collagen Type I / Q000652:urine; D005260:Female; D005272:Femur Neck / Q000378:metabolism; D008159:Lumbar Vertebrae / Q000378:metabolism; D008252:Macaca fascicularis / Q000502:physiology*; D010052:Ovariectomy / Q000009:adverse effects*; D011884:Radius / Q000378:metabolism; D011897:Random Allocation
D024042:Collagen Type I; D000469:Alkaline Phosphatase