Non-association between polymorphisms of the frizzled receptor genes and bone mineral density in postmenopausal Korean women.
We investigated the association between single nucleotide polymorphisms (SNPs) in the frizzled (FZD) genes in the Wnt signal pathway and circulating osteoprotegerin (OPG), soluble receptor activator of NF-kappaB ligand (sRANKL) levels, bone turnover markers, and bone mineral density (BMD) in postmenopausal women. The SNPs in the FZD1, FZD5, FZD6, FZD7, and FZD9 genes were analyzed by direct sequencing in 371 postmenopausal Korean women. Levels of serum OPG, sRANKL, osteocalcin, C-telopeptide of type I collagen, calcium, parathyroid hormone and calcitonin, and BMD at the lumbar spine and femoral neck were measured. The SNPs in the FZD1, FZD5, FZD7, and FZD9 genes, and in exon 2 of the FZD6 gene were not observed. No significant differences in the adjusted BMD of lumbar spine and femoral neck and serum levels of OPG, sRANKL, and bone markers were noted among the single or haplotype genotypes of the L345M and E664A SNPs in the FZD6 gene and the distributions of these single or haplotype genotypes were not different according to the bone mass status. In conclusion, the polymorphisms of the FZD genes are not associated with BMD of the lumbar spine and femoral neck, bone turnover markers, or circulating OPG-sRANKL in Korean women.
Kim
Jung Gu
JG
Department of Obstetrics and Gynecology, College of Medicine, Seoul National University, Seoul, Korea. kimjg@plaza.snu.ac.kr
Kim
Hoon
H
Jee
Byung Chul
BC
Suh
Chang Suk
CS
Choi
Young Min
YM
Moon
Shin Yong
SY
D000368:Aged; D000483:Alleles; D044466:Asian People / Q000235:genetics*; D015415:Biomarkers / Q000032:analysis; D015519:Bone Density / Q000235:genetics*; D005260:Female; D051157:Frizzled Receptors / Q000235:genetics*; D005787:Gene Frequency; D005838:Genotype; D006801:Humans; D008875:Middle Aged; D053244:Osteoprotegerin / Q000378:metabolism; D020641:Polymorphism, Single Nucleotide; D017698:Postmenopause; D053246:Receptor Activator of Nuclear Factor-kappa B / Q000378:metabolism
D015415:Biomarkers; D051157:Frizzled Receptors; D053244:Osteoprotegerin; D053246:Receptor Activator of Nuclear Factor-kappa B