title: Resveratrol, a natural antioxidant, attenuates intestinal ischemia/reperfusion injury in rats.
abstract: The intestine is highly susceptible to ischemia/reperfusion (I/R) injury. Splanchnic ischemia is the initial event that releases injurious factors, leading to systemic disorders with high morbidity and mortality. Oxidative stress mediators are believed to contribute to the intestinal I/R injury. Resveratrol, a polyphenol found in grapes, is shown to be a strong antioxidant in various tissues, with a property of an estrogen-receptor agonist. Therefore, we investigated the effects of resveratrol on oxidative injury in the intestine. Female Wistar rats were randomly allocated into four groups (n = 8, each). The sham group was only subjected to surgical procedures, while other animals were subjected to intestinal ischemia (60 min) and subsequent reperfusion (60 min). One group received resveratrol (15 mg/kg, 0.3 ml/day intraperitoneally) for both 5 days before surgery and 15 min before ischemia, while the other was treated intraperitoneally with 0.5% ethyl alcohol as vehicle (0.3 ml/day). In the I/R rat intestines, we detected severe tissue injuries (p < 0.001), the significant increases in the tissue levels of malondialdehyde (MDA), nitric oxide (NO), and myeloperoxidase (MPO) (p < 0.001), and the decrease in superoxide dismutase (SOD) activity (p < 0.001), compared to the sham control. Resveratrol significantly ameliorated the intestinal injury, decreased MDA, NO and MPO levels to the sham control levels, and decreased bacterial translocation in mesentery lymph nodes, liver and spleen (p < 0.001). Resveratrol also restored the SOD activity. These results suggest that resveratrol could protect intestinal tissue against I/R injury with its potent antioxidant properties.
authors: Department of General Surgery, Faculty of Medicine, Mustafa Kemal University, Hatay, Turkey. veliorhan@hotmail.com
authors: Orhan Veli
authors: OV
authors: Ozkan
authors: Mehmet Fatih
authors: MF
authors: Yuzbasioglu
authors: Harun
authors: H
authors: Ciralik
authors: Ergul Belge
authors: EB
authors: Kurutas
authors: Zafer
authors: Z
authors: Yonden
authors: Mehmet
authors: M
authors: Aydin
authors: Ertan
authors: E
authors: Bulbuloglu
authors: Ersan
authors: E
authors: Semerci
authors: Mustafa
authors: M
authors: Goksu
authors: Yalcin
authors: Y
authors: Atli
authors: Vedat
authors: V
authors: Bakan
authors: Nizami
authors: N
authors: Duran
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism* / Q000494:pharmacology; D018988:Bacterial Translocation / Q000187:drug effects; D005260:Female; D016166:Free Radical Scavengers / Q000378:metabolism; D007413:Intestinal Mucosa / Q000378:metabolism / Q000473:pathology; D007422:Intestines / Q000187:drug effects / Q000473:pathology; D008315:Malondialdehyde / Q000378:metabolism; D009566:Nitrates / Q000032:analysis; D009569:Nitric Oxide / Q000378:metabolism; D009573:Nitrites / Q000032:analysis; D009195:Peroxidase / Q000378:metabolism; D011506:Proteins / Q000032:analysis; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000378:metabolism* / Q000473:pathology; D000077185:Resveratrol; D013267:Stilbenes / Q000378:metabolism* / Q000494:pharmacology; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D000975:Antioxidants; D016166:Free Radical Scavengers; D009566:Nitrates; D009573:Nitrites; D011506:Proteins; D013267:Stilbenes; D009569:Nitric Oxide; D008315:Malondialdehyde; D009195:Peroxidase; D013482:Superoxide Dismutase; D000077185:Resveratrol