title: A novel technique to reconstruct a boxlike bone defect in the mandible and support dental implants with In vivo tissue-engineered bone.
abstract: The purpose of this study was to investigate the feasibility of using in vivo tissue-engineered (TE) bone to repair boxlike mandibular defect and support dental implant, and then provide experimental evidence for the future application of the novel technique in the clinical setting. The TE bone graft was constructed in vivo by implanting osteoinductive calcium phosphate (Ca-P) ceramics in the femoral muscles of dog for 8 weeks, then was transplanted to repair the autogeneic boxlike bone defect site created in one side of the mandible and simultaneously support a dental implant, while in the opposite side of the mandibular defect, the same ceramic was used directly as control. 8 weeks after transplantation, samples were harvested for analysis. The results demonstrated that the technique of in vivo tissue engineering improved the mechanical and biologic properties of ceramics significantly. After transplantation, the in vivo TE ceramic-bone grafts were involved in bone metabolism of the host and fused well with the host bone. The dental implants were stable and had been integrated with both TE bone grafts and autologous bone. Therefore, it is feasible to construct a live bone graft with osteoinductive Ca-P ceramics in vivo, then repair a mandibular bone defect, and support a dental implant. In conclusion, in vivo TE bone is a promising technique for bone repair.
authors: State Key Laboratory of Oral Diseases, Sichuan University, Chengdu 610044, People's Republic of China.|West China Hospital of Stomatology, Sichuan University, Chengdu 610041, People's Republic of China.
authors: Jinfeng
authors: J
authors: Yao
authors: State Key Laboratory of Oral Diseases, Sichuan University, Chengdu 610044, People's Republic of China.
authors: Xiaoyu
authors: X
authors: Li
authors: State Key Laboratory of Oral Diseases, Sichuan University, Chengdu 610044, People's Republic of China.
authors: Chongyun
authors: C
authors: Bao
authors: National Engineering Research Center for Biomaterials, Sichuan University, Chengdu 610065, People's Republic of China.
authors: Hongsong
authors: H
authors: Fan
authors: National Engineering Research Center for Biomaterials, Sichuan University, Chengdu 610065, People's Republic of China.
authors: Xingdong
authors: X
authors: Zhang
authors: State Key Laboratory of Oral Diseases, Sichuan University, Chengdu 610044, People's Republic of China.
authors: Zhiqing
authors: Z
authors: Chen
mesh_terms: D000818:Animals; D018786:Bone Substitutes; D016025:Bone Transplantation; D001842:Bone and Bones / Q000002:abnormalities*; D002130:Calcium Phosphates; D002516:Ceramics; D015921:Dental Implants; D004285:Dogs; D008297:Male; D008334:Mandible / Q000002:abnormalities*; D008422:Materials Testing; D008855:Microscopy, Electron, Scanning; D009132:Muscles / Q000166:cytology / Q000648:ultrastructure; D019275:Radiopharmaceuticals; D013669:Technetium Tc 99m Medronate; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds; D015899:Tomography, Emission-Computed, Single-Photon
chemical_list: D018786:Bone Substitutes; D002130:Calcium Phosphates; D015921:Dental Implants; D019275:Radiopharmaceuticals; D013669:Technetium Tc 99m Medronate