Microbial chlorination of organic matter in forest soil: investigation using 36Cl-chloride and its methodology.
Chloride, which comes into the forest ecosystem largely from the sea as aerosol (and has been in the past assumed to be inert), causes chlorination of soil organic matter. Studies of the chlorination showed that the content of organically bound chlorine in temperate forest soils is higher than that of chloride, and various chlorinated compounds are produced. Our study of chlorination of organic matter in the fermentation horizon of forest soil using radioisotope 36Cl and tracer techniques shows that microbial chlorination clearly prevails over abiotic, chlorination of soil organic matter being enzymatically mediated and proportional to chloride content and time. Long-term (>100 days) chlorination leads to more stable chlorinated substances contained in the organic layer of forest soil (overtime; chlorine is bound progressively more firmly in humic acids) and volatile organochlorines are formed. Penetration of chloride into microorganisms can be documented by the freezing/thawing technique. Chloride absorption in microorganisms in soil and in litter residues in the fermentation horizon complicates the analysis of 36Cl-chlorinated soil. The results show that the analytical procedure used should be tested for every soil type under study.
Rohlenová
J
J
Institute of Experimental Botany, Academy of Sciences of the Czech Republic, CZ-14220 Prague, Czech Republic.
Gryndler
M
M
Forczek
S T
ST
Fuksová
K
K
Handova
V
V
Matucha
M
M
D001419:Bacteria / Q000378:metabolism*; D001673:Biodegradation, Environmental; D002712:Chlorides / Q000032:analysis / Q000737:chemistry*; D052918:Environmental Restoration and Remediation / Q000379:methods*; D005615:Freezing; D054879:Halogenation; D009930:Organic Chemicals / Q000378:metabolism*; D012987:Soil / Q000032:analysis*; D013242:Sterilization; D013997:Time Factors; D014197:Trees / Q000382:microbiology*
D002712:Chlorides; D009930:Organic Chemicals; D012987:Soil