Mitochondrial superoxide radicals differentially affect muscle activity and neural function.
Cellular superoxide radicals (O(2)(-)) are mostly generated during mitochondrial oxygen metabolism. O(2)(-) serves as the raw material for many reactive oxygen species (ROS) members like H(2)O(2) and OH(.-) radicals following its catalysis by superoxide dismutase (SOD) enzymes and also by autocatalysis (autodismutation) reactions. Mitochondrial ROS generation could have serious implications on degenerative diseases. In model systems overproduction of mitochondrial O(2)(-) resulting from the loss of SOD2 function leads to movement disorders and drastic reduction in life span in vertebrates and invertebrates alike. With the help of a mitochondrial SOD2 loss-of-function mutant, Sod2(n283), we measured the sensitivity of muscles and neurons to ROS attack. Neural outputs from flight motor neurons and sensory neurons were unchanged in Sod2(n283) and the entire neural circuitry between the giant fiber (GF) and the dorsal longitudinal muscles (DLM) showed no overt defect due to elevated ROS. Such insensitivity of neurons to mitochondrial superoxides was further established through neuronal expression of SOD2, which failed to improve survival or locomotive ability of Sod2(n283). On the other hand, ultrastructural analysis of Sod2(n283) muscles revealed fewer mitochondria and reduced muscle ATP production. By targeting the SOD2 expression to the muscle we demonstrate that the early mortality phenotype of Sod2(n283) can be ameliorated along with signs of improved mobility. In summary, muscles appear to be more sensitive to superoxide attack relative to the neurons and such overt phenotypes observed in SOD2-deficient animals can be directly attributed to the muscle.
Godenschwege
Tanja
T
Department of Biological Sciences, Florida Atlantic University, Boca Raton, Florida 33431, USA.
Forde
Renée
R
Davis
Claudette P
CP
Paul
Anirban
A
Beckwith
Kristopher
K
Duttaroy
Atanu
A
D000818:Animals; D030801:Animals, Genetically Modified; D002470:Cell Survival / Q000235:genetics; D004331:Drosophila melanogaster / Q000235:genetics / Q000378:metabolism / Q000502:physiology; D008928:Mitochondria / Q000378:metabolism*; D008954:Models, Biological; D009046:Motor Neurons / Q000378:metabolism / Q000502:physiology; D018482:Muscle, Skeletal / Q000187:drug effects* / Q000378:metabolism; D009469:Neuromuscular Junction / Q000235:genetics / Q000502:physiology; D009474:Neurons / Q000187:drug effects* / Q000378:metabolism / Q000502:physiology; D010641:Phenotype; D020419:Photoreceptor Cells, Vertebrate / Q000378:metabolism / Q000502:physiology; D013482:Superoxide Dismutase / Q000235:genetics / Q000378:metabolism; D013481:Superoxides / Q000378:metabolism / Q000494:pharmacology*; D009435:Synaptic Transmission / Q000235:genetics; D013997:Time Factors; D000099156:Superoxide Dismutase 2
D013482:Superoxide Dismutase; D013481:Superoxides; D000099156:Superoxide Dismutase 2