title: [Effect of t10, c12-conjugated linoleic acid on fatty acid metabolism in C2C12 myotubes].
abstract: OBJECTIVE
  To study the effect of t10, c12-conjugated linoleic acid (t10, c12-CLA) on fatty acid metabolism in myotubes under the high free fatty acid condition.
  
  
  METHODS
  We used palmitate to simulate a high free fatty acid condition for C2C12 myotubes in vitro and administered t10, c12-CLA across the groups. The expression and translocation of FAT/CD36 were determined by fluorimetric immunifaction using laser scanning confocal microscope. A series of enzymes related to fatty acid metabolism, together with AMP-activated protein kinase (AMPK), were determined by western blot.
  
  
  RESULTS
  t10, c12-CLA dose-dependently increased the translocation of FAT/CD36 to cellular membranes, and increased the expression of p-ACC and CPT-1 in myotubes under the high free fatty acid condition. Moreover, t10, c12-CLA activated the AMPK in myotubes.
  
  
  CONCLUSIONS
  CLA can increased the fatty acid oxidation in myotubes via activating AMPK.
authors: Department of Nutrition and Food Hygiene, School of Public Health, Harbin Medical University, Harbin 150086, China. qinhong_1104@yahoo.com.cn
authors: Hong
authors: H
authors: Qin
authors: Ying
authors: Y
authors: Li
authors: Lin
authors: L
authors: Shi
authors: Changhao
authors: C
authors: Sun
mesh_terms: D055372:AMP-Activated Protein Kinases / Q000378:metabolism*; D000818:Animals; D018955:CD36 Antigens / Q000378:metabolism; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D005227:Fatty Acids / Q000378:metabolism*; D044243:Linoleic Acids, Conjugated / Q000494:pharmacology*; D051379:Mice; D018485:Muscle Fibers, Skeletal / Q000166:cytology / Q000378:metabolism*
chemical_list: D018955:CD36 Antigens; D005227:Fatty Acids; D044243:Linoleic Acids, Conjugated; C496197:trans-10,cis-12-conjugated linoleic acid; D055372:AMP-Activated Protein Kinases