title: Microscopic investigation of reversible nanoscale surface size dependent protein conjugation.
abstract: Aβ(1-40) coated 20 nm gold colloidal nanoparticles exhibit a reversible color change as pH is externally altered between pH 4 and 10. This reversible process may contain important information on the initial reversible step reported for the fibrillogenesis of Aβ (a hallmark of Alzheimer's disease). We examined this reversible color change by microscopic investigations. AFM images on graphite surfaces revealed the morphology of Aβ aggregates with gold colloids. TEM images clearly demonstrate the correspondence between spectroscopic features and conformational changes of the gold colloid.
authors: Department of Chemistry, The State University of New York at Geneseo, 1 College Circle, Geneseo, NY 14454, USA.
authors: Kazushige
authors: K
authors: Yokoyama
authors: Department of Chemistry, The State University of New York at Geneseo, 1 College Circle, Geneseo, NY 14454, USA.
authors: Hyunah
authors: H
authors: Cho
authors: Department of Chemistry, The State University of New York at Geneseo, 1 College Circle, Geneseo, NY 14454, USA.
authors: Sean P
authors: SP
authors: Cullen
authors: Department of Chemistry, The State University of New York at Geneseo, 1 College Circle, Geneseo, NY 14454, USA.
authors: Matthew
authors: M
authors: Kowalik
authors: Department of Chemistry, The State University of New York at Geneseo, 1 College Circle, Geneseo, NY 14454, USA.
authors: Nicole M
authors: NM
authors: Briglio
authors: Department of Biology, The State University of New York at Geneseo, 1 College Circle, Geneseo, NY 14454, USA.
authors: Harold J
authors: HJ
authors: Hoops
authors: College of Nanoscale Science and Engineering, University of Albany-SUNY, 255 Fuller Road, Albany, NY 12203, USA.
authors: Zhouying
authors: Z
authors: Zhao
authors: College of Nanoscale Science and Engineering, University of Albany-SUNY, 255 Fuller Road, Albany, NY 12203, USA.
authors: Michael A
authors: MA
authors: Carpenter
mesh_terms: D000544:Alzheimer Disease / Q000378:metabolism; D016229:Amyloid beta-Peptides / Q000096:biosynthesis / Q000737:chemistry*; D017979:Gold Colloid / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry*; D018625:Microscopy, Atomic Force; D046529:Microscopy, Electron, Transmission; D010047:Ovalbumin / Q000737:chemistry; D010446:Peptide Fragments / Q000096:biosynthesis / Q000737:chemistry*; D011487:Protein Conformation; D011506:Proteins / Q000378:metabolism; D014867:Water / Q000737:chemistry
chemical_list: D016229:Amyloid beta-Peptides; D017979:Gold Colloid; D010446:Peptide Fragments; D011506:Proteins; C078592:amyloid beta-protein (1-40); D014867:Water; D010047:Ovalbumin