title: Exercise-induced up-regulation of MMP-1 and IL-8 genes in endurance horses.
abstract: BACKGROUND
  The stress response is a critical factor in the training of equine athletes; it is important for performance and for protection of the animal against physio-pathological disorders.In this study, the molecular mechanisms involved in the response to acute and strenuous exercise were investigated using peripheral blood mononuclear cells (PBMCs).
  
  
  RESULTS
  Quantitative real-time PCR (qRT-PCR) was used to detect modifications in transcription levels of the genes for matrix metalloproteinase-1 (MMP-1) and interleukin 8 (IL-8), which were derived from previous genome-wide expression analysis. Significant up-regulation of these two genes was found in 10 horses that had completed a race of 90-120 km in a time-course experimental design.
  
  
  CONCLUSIONS
  These results suggest that MMP-1 and IL-8 are both involved in the exercise-induced stress response, and this represents a starting point from which to understand the adaptive responses to this phenomenon.
authors: Pathology, Diagnostic and Veterinary Clinic Department, University of Perugia, Via San Costanzo 4, 06126 Perugia, Italy. katia.cappelli@unipg.it
authors: Katia
authors: K
authors: Cappelli
authors: Michela
authors: M
authors: Felicetti
authors: Stefano
authors: S
authors: Capomaccio
authors: Camillo
authors: C
authors: Pieramati
authors: Maurizio
authors: M
authors: Silvestrelli
authors: Andrea
authors: A
authors: Verini-Supplizi
mesh_terms: D000818:Animals; D006736:Horses / Q000502:physiology*; D016209:Interleukin-8 / Q000502:physiology*; D020781:Matrix Metalloproteinase 1 / Q000502:physiology*; D010805:Physical Conditioning, Animal / Q000379:methods*; D010807:Physical Endurance / Q000502:physiology*; D013312:Stress, Physiological / Q000502:physiology*; D015854:Up-Regulation / Q000502:physiology
chemical_list: D016209:Interleukin-8; D020781:Matrix Metalloproteinase 1