[Isolation and analysis of syringin in cell suspension cultures of Saussurea involucrata].
Syringin is one of the main bioactive ingredients in Saussurea involucrata. In this study, various chromatographic techniques were employed to isolate and purify syringin in the polar extraction of cell suspension cultures of S. involucrata. The structure of syringin was characterized by the analysis of spectroscopic data. A quantitative analytical method for the content of syringin in cultures of S. involucrata was established with RP-HPLC. The method is convenient, accurate and reliable. All this results provided a basis for further studies on application of cell suspension cultures of S. involucrata for large-scale production of bioactive compound syringin.
Chen
Ri-dao
RD
Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China.
Zou
Jian-hua
JH
Jia
Jing-ming
JM
Dai
Jun-gui
JG
D018929:Cell Culture Techniques; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005960:Glucosides / Q000032:analysis* / Q000302:isolation & purification*; D010666:Phenylpropionates / Q000032:analysis* / Q000302:isolation & purification*; D010946:Plants, Medicinal / Q000737:chemistry / Q000166:cytology; D015203:Reproducibility of Results; D030441:Saussurea / Q000737:chemistry* / Q000166:cytology
D005960:Glucosides; D010666:Phenylpropionates; C028305:syringin