title: [Effect of hydroquinone on the expression of ubiquitin-conjugating enzyme Rad6B in human L-02 hepatic cells].
abstract: OBJECTIVE
  To study the effects of hydroquinone (HQ) on expression of ubiquitin-conjugating enzyme Rad6B in human L-02 hepatic cells.
  
  
  METHODS
  After L-02 hepatic cells were at the concentrations of 0, 5, 10, 20, 40, 80, and 160 micromol/L HQ for 24h, and the levels of Rad6B mRNA and protein expressions in L-02 hepatic cells were detected by real-time fluorescent quantitative polymerase chain reaction (QPCR) technique and Western blot method respectively.
  
  
  RESULTS
  It was found that HQ in the range of 0-160 micromol/L could induce increases in the expressions of Rad6B mRNA and protein, which was in a dose-dependent manner. The levels of Rad6B mRNA and protein expressions in the treated group of 5, 10, 20, 40, 80, and 160 micromol/L were higher than those in the control (P < 0.01), levels of Rad6B mRNA and protein expressions reached the maximun when L-02 hepatic cells were treated by HQ at the concentrations of 160 micromol/L, and the relative quantity values were 4.35 and 0.85, respectively.
  
  
  CONCLUSIONS
  HQ could regulate up the expression of Rad6B in L-02 hepatic cells.
authors: Department of Preventive Medicine, Gannan Medical College, Ganzhou 341000, China. hgh0129@sohu.com
authors: Gonghua
authors: G
authors: Hu
authors: Zhixiong
authors: Z
authors: Zhuang
authors: Jianping
authors: J
authors: Yang
authors: Haiyan
authors: H
authors: Huang
mesh_terms: D002460:Cell Line; D022781:Hepatocytes / Q000166:cytology / Q000201:enzymology*; D006801:Humans; D006873:Hydroquinones / Q000633:toxicity*; D009153:Mutagens / Q000633:toxicity; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D044763:Ubiquitin-Conjugating Enzymes / Q000235:genetics / Q000378:metabolism*; D015854:Up-Regulation / Q000187:drug effects
chemical_list: D006873:Hydroquinones; D009153:Mutagens; D012333:RNA, Messenger; C471546:UBE2B protein, human; D044763:Ubiquitin-Conjugating Enzymes; C031927:hydroquinone