Inactivation of a transgene due to transposition of insertion sequence (IS136) of Agrobacterium tumefaciens.
Agrobacterium strains harbour insertion sequences, which are known to transpose into genomes as well as into Ti plasmids. In this study we report the inactivation of a transgene due to transposition of the A. tumefaciens insertion sequence IS136. The transposition was discovered following transformation of plant tissues, although the fidelity of the binary vector was confirmed following transformation into Agrobacterium. Such transpositions are rare but can occur and it is thus important to check the fidelity of the binary vector at different times of Agrobacterium growth in order to avoid failure in achieving transgene expression.
Rawat
Preeti
P
Department of Genetics, University of Delhi South Campus, Benito Juarez Road, New Delhi 110021, India.
Kumar
Sanjeev
S
Pental
Deepak
D
Burma
Pradeep Kumar
PK
D016960:Agrobacterium tumefaciens / Q000235:genetics*; D017931:DNA Primers; D004251:DNA Transposable Elements; D018076:DNA, Complementary / Q000378:metabolism; D005798:Genes, Bacterial; D005822:Genetic Vectors; D008957:Models, Genetic; D010944:Plants / Q000382:microbiology*; D012313:RNA / Q000378:metabolism; D014169:Transformation, Bacterial; D019076:Transgenes
D017931:DNA Primers; D004251:DNA Transposable Elements; D018076:DNA, Complementary; D012313:RNA