title: The performance of dental pulp stem cells on nanofibrous PCL/gelatin/nHA scaffolds.
abstract: The aim of current study is to investigate the in vitro and in vivo behavior of dental pulp stem cells (DPSCs) seeded on electrospun poly(epsilon-caprolactone) (PCL)/gelatin scaffolds with or without the addition of nano-hydroxyapatite (nHA). For the in vitro evaluation, DNA content, alkaline phosphatase (ALP) activity and osteocalcin (OC) measurement showed that the scaffolds supported DPSC adhesion, proliferation, and odontoblastic differentiation. Moreover, the presence of nHA upregulated ALP activity and promoted OC expression. Real-time PCR data confirmed these results. SEM micrographs qualitatively confirmed the proliferation and mineralization characteristics of DPSCs on both scaffolds. Subsequently, both scaffolds seeded with DPSCs were subcutaneously implanted into immunocompromised nude mice. Scaffolds with nHA but without cells were implanted as control. Histological evaluation revealed that all implants were surrounded by a thin fibrous tissue capsule without any adverse effects. The cell/scaffold composites showed obvious in vivo hard tissue formation, but there was no sign of tissue ingrowth. Further, the combination of nHA in scaffolds did upregulate the expression of specific odontogenic genes. In conclusion, the incorporation of nHA in nanofibers indeed enhanced DPSCs differentiation towards an odontoblast-like phenotype in vitro and in vivo.
authors: School and Hospital of Stomatology, Wuhan University, Wuhan 430079, Hubei Province, People's Republic of China.
authors: Xuechao
authors: X
authors: Yang
authors: Fang
authors: F
authors: Yang
authors: X Frank
authors: XF
authors: Walboomers
authors: Zhuan
authors: Z
authors: Bian
authors: Mingwen
authors: M
authors: Fan
authors: John A
authors: JA
authors: Jansen
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects; D048430:Cell Shape / Q000187:drug effects; D002478:Cells, Cultured; D003782:Dental Pulp / Q000166:cytology*; D017886:Durapatite / Q000494:pharmacology*; D005780:Gelatin / Q000494:pharmacology*; D005786:Gene Expression Regulation / Q000187:drug effects; D020007:Implants, Experimental; D051379:Mice; D008819:Mice, Nude; D057139:Nanofibers / Q000737:chemistry* / Q000648:ultrastructure; D015675:Osteocalcin / Q000235:genetics / Q000378:metabolism; D011091:Polyesters / Q000494:pharmacology*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013234:Stem Cells / Q000166:cytology* / Q000187:drug effects* / Q000201:enzymology / Q000648:ultrastructure; D054457:Tissue Scaffolds / Q000737:chemistry; D014961:X-Ray Diffraction
chemical_list: D011091:Polyesters; D012333:RNA, Messenger; D015675:Osteocalcin; C016240:polycaprolactone; D005780:Gelatin; D017886:Durapatite; D000469:Alkaline Phosphatase