Wireless, remote-query, and high sensitivity Escherichia coli O157:H7 biosensor based on the recognition action of concanavalin A.
Escherichia coli O157:H7 is detected using a remote-query (wireless, passive) magnetoelastic sensor platform to which a 1 microm thick layer of Bayhydrol 110 and then a layer of functionalized mannose is applied. The multivalent binding of lectin concanavalin A (Con A) to the E. coli surface O-antigen and mannose favors the strong adhesion of E. coli to the mannose-modified magnetoelastic sensor; E. coli is rigidly and strongly attached on the mannose-modified sensor through Con A, which works as a bridge to bind E. coli to the mannose-modified sensor surface. As E. coli is bound to the sensor, its resonance frequency shifts, enabling quantification of E. coli concentration with a limit of detection of 60 cells/mL and a linear logarithmic response range of 6.0 x 10(1) to 6.1 x 10(9) cells/mL. The analysis can be directly conducted without incubation and completed in 3 h or less.
Lu
Qingzhu
Q
State Key Laboratory of Chemo/Biosensing and Chemometrics, Department of Chemistry, Hunan University, Changsha 410082, P.R. China.
Lin
Hailan
H
Ge
Shutian
S
Luo
Shenglian
S
Cai
Qingyun
Q
Grimes
Craig A
CA
D000327:Adsorption; D001207:Asepsis; D015374:Biosensing Techniques / Q000379:methods*; D002118:Calcium / Q000378:metabolism; D003208:Concanavalin A / Q000378:metabolism*; D019453:Escherichia coli O157 / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism*; D008280:Magnetics; D008345:Manganese / Q000378:metabolism; D008358:Mannose / Q000378:metabolism; D019081:O Antigens / Q000378:metabolism; D013379:Substrate Specificity; D013997:Time Factors
D019081:O Antigens; D003208:Concanavalin A; D008345:Manganese; D008358:Mannose; D002118:Calcium