[Establishment of coronary heart disease model of phlegm-stasis cementation syndrome type in mini-swines].
OBJECTIVE
To establish a disease-syndrome conjugated animal model, the mini-swine coronary heart disease (CHD) model of phlegm-stasis cementation syndrome type, by high fat diet feeding and coronary artery balloon injury.


METHODS
Mini-swine were randomly divided into the control group and the model group, 6 in each group. They were fed with common forage and high fat forage respectively for 10 weeks and the coronary left anterior descending branch in the model group was injured by balloon intervention technique after 2-week feeding to establish CHD model. The model establishment and its physiopathological indices were evaluated by examinations on body mass index (BMI), blood levels of lipid, high-sensitivity C-reactive protein (hs-CRP), body surface electrocardiograph (BS-ECG), coronary angiography and pathological indices.


RESULTS
BMI, total cholesterol (TC), triglyceride (TG), low-density lipoprotein (LDL-C), hs-CRP, sigma-ST and N-ST indicated by BS-ECG in the model group were all higher than those in the control group respectively (P < 0.05 or P < 0.01). Coronary angiography showed coronary lumen narrowed with apparent lumen loss, showing a significant difference as compared with the control group (P < 0.01). In EVG staining, the diameters of lumen in the model group was obviously narrow with intima proliferation, also significantly different to those in the control group (P < 0.01).


CONCLUSIONS
Disease-syndrome combined model for coronary heart disease of phlegm-stasis cementation syndrome type in mini-swine could be established by high fat diet feeding with coronary arterial injury.
Li
Xin-zhi
XZ
Experimental Research Center, Xiyuan Hospital, China Academy of Chinese Medical Sciences, Beijing.
Liu
Jian-xun
JX
Ren
Jian-xun
JX
D000818:Animals; D003327:Coronary Disease / Q000209:etiology; D003937:Diagnosis, Differential; D004041:Dietary Fats / Q000008:administration & dosage; D004195:Disease Models, Animal; D005260:Female; D008297:Male; D008516:Medicine, Chinese Traditional / Q000379:methods*; D011897:Random Allocation; D013552:Swine; D013556:Swine, Miniature; D013577:Syndrome
D004041:Dietary Fats