Unequally redundant RCD1 and SRO1 mediate stress and developmental responses and interact with transcription factors.
RADICAL-INDUCED CELL DEATH1 (RCD1) is an important regulator of stress and hormonal and developmental responses in Arabidopsis thaliana. Together with its closest homolog, SIMILAR TO RCD-ONE1 (SRO1), it is the only Arabidopsis protein containing the WWE domain, which is known to mediate protein-protein interactions in other organisms. Additionally, these two proteins contain the core catalytic region of poly-ADP-ribose transferases and a conserved C-terminal domain. Tissue and subcellular localization data indicate that RCD1 and SRO1 have partially overlapping functions in plant development. In contrast mutant data indicate that rcd1 has defects in plant development, whereas sro1 displays normal development. However, the rcd1 sro1 double mutant has severe growth defects, indicating that RCD1 and SRO1 exemplify an important genetic principle - unequal genetic redundancy. A large pair-wise interaction test against the REGIA transcription factor collection revealed that RCD1 interacts with a large number of transcription factors belonging to several protein families, such as AP2/ERF, NAC and basic helix-loop-helix (bHLH), and that SRO1 interacts with a smaller subset of these. Full genome array analysis indicated that in many cases targets of these transcription factors have altered expression in the rcd1 but not the sro1 mutant. Taken together RCD1 and SRO1 are required for proper plant development.
Jaspers
Pinja
P
Plant Biology, Department of Biological and Environmental Sciences, Viikki Biocenter, University of Helsinki, Helsinki, Finland.
Blomster
Tiina
T
Brosché
Mikael
M
Salojärvi
Jarkko
J
Ahlfors
Reetta
R
Vainonen
Julia P
JP
Reddy
Ramesha A
RA
Immink
Richard
R
Angenent
Gerco
G
Turck
Franziska
F
Overmyer
Kirk
K
Kangasjärvi
Jaakko
J
D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D018507:Gene Expression Regulation, Developmental; D018506:Gene Expression Regulation, Plant; D018745:Genome, Plant; D008969:Molecular Sequence Data; D009154:Mutation; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism*; D020411:Oligonucleotide Array Sequence Analysis; D030821:Plants, Genetically Modified / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D011401:Promoter Regions, Genetic; D018749:RNA, Plant / Q000235:genetics; D016415:Sequence Alignment; D013312:Stress, Physiological; D014157:Transcription Factors / Q000378:metabolism*
D029681:Arabidopsis Proteins; D009687:Nuclear Proteins; C492758:RCD1 protein, Arabidopsis; D018749:RNA, Plant; C557703:SRO1 protein, Arabidopsis; D014157:Transcription Factors