Time-dependent differential gene expression in lysophosphatidic acid-treated young and senescent human diploid fibroblasts.
The gene expression profiles of lysophosphatidic acid (LPA)-treated young and senescent human diploid fibroblasts (HDFs) were examined using cDNA microarray analysis. The expression of some genes, including EGR 1/3 and MRRF, was controlled by LPA similarly in young and senescent cells, showing a typical time-dependent up-and-down expression profile. In contrast, some other genes, including DUSP6, CYR61, and F3, showed sustained upregulation in senescent HDFs later after LPA treatment. These genes might be involved in altered LPA responsiveness during the aging process.
Rhim
Ji-Heon
JH
Department of Biochemistry and Molecular Biology, Ageing and Apoptosis Research Center, Seoul National University College of Medicine, Chongno-Gu, South Korea.
Jang
Ik-Soon
IS
Choi
Jong-Soon
JS
Kwon
Hyun Jin
HJ
Yeo
Eui-Ju
EJ
Park
Sang Chul
SC
D002478:Cells, Cultured; D016922:Cellular Senescence / Q000502:physiology*; D018076:DNA, Complementary / Q000235:genetics; D004171:Diploidy; D005347:Fibroblasts / Q000378:metabolism*; D015870:Gene Expression / Q000187:drug effects*; D020869:Gene Expression Profiling; D006801:Humans; D008246:Lysophospholipids / Q000494:pharmacology*; D046228:Microarray Analysis; D013997:Time Factors
D018076:DNA, Complementary; D008246:Lysophospholipids; C032881:lysophosphatidic acid