Resonance Raman spectroscopy.
Resonance Raman spectroscopy may yield precise information on the conformation of, and on the interactions assumed by, the chromophores involved in the first steps of the photosynthetic process, whether isolated in solvents, embedded in soluble or membrane proteins, or, as shown recently, in vivo. By making use of this technique, it is possible, for instance, to relate the electronic properties of these molecules to their structure and/or the physical properties of their environment, or to determine subtle changes of their conformation associated with regulatory processes. After a short introduction to the physical principles that govern resonance Raman spectroscopy, the information content of resonance Raman spectra of chlorophyll and carotenoid molecules is described in this review, together with the experiments which helped in determining which structural parameter each Raman band is sensitive to. A selection of applications of this technique is then presented, in order to give a fair and precise idea of which type of information can be obtained from its use in the field of photosynthesis.
Robert
Bruno
B
Institute of Biology and Technology of Saclay, Commissariat à l'Energie Atomique, URA 2096 Centre National de la Recherche Scientifique, Gif sur Yvette, France. bruno.robert@cea.fr
D010788:Photosynthesis; D010860:Pigments, Biological / Q000378:metabolism; D013059:Spectrum Analysis, Raman / Q000295:instrumentation / Q000379:methods*
D010860:Pigments, Biological