[Quantitative determination of 1-deoxynojirimycin in mulberry leaves by high-performance liquid chromatographic-tandem mass/mass spectrometry].
OBJECTIVE
To develop a high-performance liquid chromatographic-tandem mass/mass spectrometric method to determine the concentration of 1-deoxynojirimycin (1-DNJ) in mulberry leaves.


METHODS
1-deoxynojirimycin was separated on an SHIMADZU HRC-NH2 column with the mobile phase consisting of acetonitrile and 0.1% formic acid. The mass spectrometric system equipped with a atmospheric pressure chemical ionization (APCI) interface was operated in Multiple Reaction Monitoring (MRM) mode.


RESULTS
The retention time of 1-deoxynojirimycin was 2.87 min, and the calibration curve was linear over a concentration range from 482 microg/L to 2410 microg/L, the average recovery was 95.8%. The detection limit was 53.6 microg/L.


CONCLUSIONS
The method is selective and sensitive for determining 1-deoxynojirimycin in mulberry leaves.
Dai
Kai-Jin
KJ
Industrial Affairs Office, Guangzhou 510515, China.
Hou
Lian-Bing
LB
Luo
Qi-Zhi
QZ
D017485:1-Deoxynojirimycin / Q000032:analysis* / Q000302:isolation & purification; D004365:Drugs, Chinese Herbal / Q000737:chemistry; D031625:Morus / Q000737:chemistry*; D018515:Plant Leaves / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D011786:Quality Control; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012996:Solutions; D053719:Tandem Mass Spectrometry
D004365:Drugs, Chinese Herbal; D012996:Solutions; D017485:1-Deoxynojirimycin