Trichinella spiralis: Effect of thymus factor X on apoptosis and necrosis in mice.
The aim of the study was to determine the effect of thymus factor X (TFX-Jelfa) on the percentage of apoptotic and necrotic lymphocytes in the spleen, mesenteric lymph nodes, and muscle tissue of mice infected with 200 larvae of Trichinella spiralis. TFX was administered subcutaneously at a dose of 15mg/kg. On days 7, 14, 21, 28, 35, 42, and 60 after infection, apoptotic and necrotic cells were detected by flow cytometry after staining with the Annexin V-Fluos Staining Kit. TFX increased the percentage of apoptotic lymphocytes in the spleen, mesenteric lymph nodes, and muscle tissue of mice infected with T. spiralis. The effect of TFX on the percentage of necrotic lymphocytes was weaker and less clear. Parasite load was lower in infected mice treated with TFX than in the untreated control mice. The effect of TFX on the host immune response and the survival of parasite larvae was therefore probably affected by the extent of inflammatory infiltrates, and not by the percentage of lymphocytes undergoing apoptosis.
Piekarska
J
J
Department of Internal and Parasitic Diseases, Wroclaw University of Environmental and Life Sciences, Poland. piekarsk@wp.pl
Michalski
A
A
Szczypka
M
M
Obmińska-Mrukowicz
B
B
D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D005434:Flow Cytometry; D008198:Lymph Nodes / Q000187:drug effects / Q000473:pathology; D008214:Lymphocytes / Q000187:drug effects* / Q000473:pathology; D008297:Male; D051379:Mice; D009132:Muscles / Q000187:drug effects / Q000469:parasitology / Q000473:pathology; D009336:Necrosis; D013154:Spleen / Q000187:drug effects / Q000473:pathology; D013949:Thymus Extracts / Q000494:pharmacology* / Q000627:therapeutic use; D017160:Trichinella spiralis / Q000187:drug effects / Q000276:immunology / Q000472:pathogenicity; D014235:Trichinellosis / Q000188:drug therapy* / Q000276:immunology / Q000473:pathology
C019108:TFX; D013949:Thymus Extracts