Chromosome 3p microsatellite allelotyping in neuroblastoma: a report on the technical hurdles.
Pinpointing critical regions of recurrent loss may help localize tumor suppressor genes. To determine the regions of loss on chromosome 3p in neuroblastoma, we performed loss of heterozygosity analysis using 16 microsatellite markers in a series of 65 primary tumors and 29 neuroblastoma cell lines. In this study, we report the results and discuss the technical hurdles that we encountered during data generation and interpretation that are of relevance for current studies or tests employing microsatellites. To provide functional support for the implication of 3p tumor suppressor genes in this childhood malignancy, we performed a microcell-mediated chromosome 3 transfer in neuroblastoma cells.
Hoebeeck
Jasmien
J
Center for Medical Genetics, Ghent University Hospital, Belgium.
De Wilde
Bram
B
Michels
Evi
E
Combaret
Valérie
V
Yigit
Nurten
N
De Smet
Els
E
Van Roy
Nadine
N
Stanbridge
Eric
E
Ru
Ning
N
Laureys
Geneviève
G
De Paepe
Anne
A
Speleman
Frank
F
Vandesompele
Jo
J
D000483:Alleles; D002460:Cell Line; D002874:Chromosome Mapping / Q000379:methods; D002893:Chromosomes, Human, Pair 3; D015972:Gene Expression Regulation, Neoplastic; D018014:Gene Transfer Techniques; D016147:Genes, Tumor Suppressor; D005838:Genotype; D006801:Humans; D019656:Loss of Heterozygosity; D018895:Microsatellite Repeats; D009447:Neuroblastoma / Q000235:genetics* / Q000473:pathology; D015203:Reproducibility of Results