A method for acetonitrile-free microcystin analysis and purification by high-performance liquid chromatography, using methanol as mobile phase.
Cyanobacteria produce a wide range of potent toxins, including hepatotoxic microcystins. HPLC methods for microcystin analysis and purification almost invariably include acetonitrile in the elution gradient mobile phase. The recent, acute, global acetonitrile shortage requires that adequate methods are available for microcystin analysis and purification without the need for acetonitrile. Here we present a convenient methanol-based method for effective HPLC analysis and purification of the toxins, with full separation of a range of microcystin variants.
Purdie
Esme L
EL
Division of Molecular Microbiology, College of Life Sciences, University of Dundee, Dow Street, Dundee DD1 5EH, Scotland, United Kingdom. e.l.purdie@dundee.ac.uk
Young
Fiona M
FM
Menzel
Diana
D
Codd
Geoffrey A
GA
D000097:Acetonitriles / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D052998:Microcystins / Q000032:analysis* / Q000302:isolation & purification; D046931:Microcystis / Q000737:chemistry; D013056:Spectrophotometry, Ultraviolet
D000097:Acetonitriles; D052998:Microcystins; C032159:acetonitrile