title: MURC/Cavin-4 and cavin family members form tissue-specific caveolar complexes.
abstract: Polymerase I and transcript release factor (PTRF)/Cavin is a cytoplasmic protein whose expression is obligatory for caveola formation. Using biochemistry and fluorescence resonance energy transfer-based approaches, we now show that a family of related proteins, PTRF/Cavin-1, serum deprivation response (SDR)/Cavin-2, SDR-related gene product that binds to C kinase (SRBC)/Cavin-3, and muscle-restricted coiled-coil protein (MURC)/Cavin-4, forms a multiprotein complex that associates with caveolae. This complex can constitutively assemble in the cytosol and associate with caveolin at plasma membrane caveolae. Cavin-1, but not other cavins, can induce caveola formation in a heterologous system and is required for the recruitment of the cavin complex to caveolae. The tissue-restricted expression of cavins suggests that caveolae may perform tissue-specific functions regulated by the composition of the cavin complex. Cavin-4 is expressed predominantly in muscle, and its distribution is perturbed in human muscle disease associated with Caveolin-3 dysfunction, identifying Cavin-4 as a novel muscle disease candidate caveolar protein.
authors: Institute for Molecular Bioscience, The University of Queensland, Brisbane, Queensland 4072, Australia.
authors: Michele
authors: M
authors: Bastiani
authors: Libin
authors: L
authors: Liu
authors: Michelle M
authors: MM
authors: Hill
authors: Mark P
authors: MP
authors: Jedrychowski
authors: Susan J
authors: SJ
authors: Nixon
authors: Harriet P
authors: HP
authors: Lo
authors: Daniel
authors: D
authors: Abankwa
authors: Robert
authors: R
authors: Luetterforst
authors: Manuel
authors: M
authors: Fernandez-Rojo
authors: Michael R
authors: MR
authors: Breen
authors: Steven P
authors: SP
authors: Gygi
authors: Jorgen
authors: J
authors: Vinten
authors: Piers J
authors: PJ
authors: Walser
authors: Kathryn N
authors: KN
authors: North
authors: John F
authors: JF
authors: Hancock
authors: Paul F
authors: PF
authors: Pilch
authors: Robert G
authors: RG
authors: Parton
mesh_terms: D041721:3T3-L1 Cells / Q000378:metabolism / Q000648:ultrastructure; D000595:Amino Acid Sequence; D000818:Animals; D021941:Caveolae / Q000378:metabolism / Q000648:ultrastructure; D022461:Caveolins / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D008565:Membrane Proteins / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D018345:Mice, Knockout; D008969:Molecular Sequence Data; D046912:Multiprotein Complexes / Q000378:metabolism*; D009124:Muscle Proteins / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D009135:Muscular Diseases / Q000378:metabolism / Q000473:pathology; D010802:Phylogeny; D020033:Protein Isoforms / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D016601:RNA-Binding Proteins; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D012508:Sarcolemma / Q000378:metabolism / Q000648:ultrastructure; D016415:Sequence Alignment
chemical_list: D022461:Caveolins; C112745:Cavin1 protein, mouse; D008565:Membrane Proteins; D046912:Multiprotein Complexes; D009124:Muscle Proteins; D020033:Protein Isoforms; D016601:RNA-Binding Proteins; D011993:Recombinant Fusion Proteins