In vitro biosynthesis of bacterial peptidoglycan using D-Cys-containing precursors: fluorescent detection of transglycosylation and transpeptidation.
Peptidoglycan precursors containing D-Cys at position 4 were polymerised to form a synthetic peptidoglycan layer, which could be fluorescently labelled, providing a new method to monitor peptidoglycan transglycosylation and transpeptidation.
Vinatier
Virginie
V
Department of Chemistry, University of Warwick, Coventry, UKCV4 7AL. T.D.Bugg@warwick.ac.uk.
Blakey
Carl B
CB
Braddick
Darren
D
Johnson
Benjamin R G
BR
Evans
Stephen D
SD
Bugg
Timothy D H
TD
D001426:Bacterial Proteins / Q000737:chemistry; D002462:Cell Membrane / Q000201:enzymology; D003545:Cysteine / Q000737:chemistry*; D004926:Escherichia coli / Q000201:enzymology; D005456:Fluorescent Dyes / Q000737:chemistry*; D006031:Glycosylation; D008856:Microscopy, Fluorescence; D009113:Muramidase / Q000737:chemistry; D009842:Oligopeptides / Q000737:chemistry*; D010457:Peptidoglycan / Q000096:biosynthesis*; D011994:Recombinant Proteins / Q000737:chemistry; D020349:Surface Plasmon Resonance
D001426:Bacterial Proteins; D005456:Fluorescent Dyes; D009842:Oligopeptides; D010457:Peptidoglycan; D011994:Recombinant Proteins; D009113:Muramidase; D003545:Cysteine