title: [MyoD mRNA expression in skeletal muscle of patients with myotonic dystrophy].
abstract: OBJECTIVE
  To explore the mRNA expression of MyoD gene in the skeletal muscles of myotonic dystrophy (MD) patients.
  
  
  METHODS
  Muscle biopsy specimens were obtained from the biceps muscles of arm of 4 MD patients and 4 healthy controls. Semi-quantitative reverse transcription polymerase chain reaction was performed to evaluate the mRNA expression of MyoD in the specimens.
  
  
  RESULTS
  The mRNA expression index of MyoD of the MD patients was (0.267 +/- 0.114), significantly lower than that of the healthy controls [(0.788 +/- 0.136), P = 0.001].
  
  
  CONCLUSIONS
  Down-regulated mRNA expression of MyoD gene is involved in the mechanism of DM.
authors: Department of Neurology, General Hospital of Chinese People's Liberation Army, Beijing 100853, China.
authors: Xiao-Ping
authors: XP
authors: Zhao
authors: Chuan-Qiang
authors: CQ
authors: Pu
authors: Feng
authors: F
authors: Duan
authors: Jie-Xiao
authors: JX
authors: Liu
authors: Yan-Ling
authors: YL
authors: Mao
authors: Ping
authors: P
authors: Luo
mesh_terms: D000293:Adolescent; D000328:Adult; D016022:Case-Control Studies; D002648:Child; D005260:Female; D015870:Gene Expression; D006801:Humans; D008297:Male; D008875:Middle Aged; D018482:Muscle, Skeletal / Q000378:metabolism*; D017570:MyoD Protein / Q000235:genetics*; D009223:Myotonic Dystrophy / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D055815:Young Adult
chemical_list: D017570:MyoD Protein; C057363:MyoD1 myogenic differentiation protein; D012333:RNA, Messenger