[Effect of low-dose 2-methoxyestradiol on the expressions of two isoforms of PRDM1 gene in myeloma].
OBJECTIVE
To investigate the effects of low-dose 2-methoxyestradiol (2ME2) on the expression of two isoforms of PRDM1 gene (PRDM1alpha with and PRDM1beta without PR-domain) during the myeloma cell differentiation.


METHODS
In myeloma cell line NCI-H929, RPMI8226, KM3 and LP-1, PRDMla and PRDM1beta transcripts were detected by real-time quantitative PCR after treatment with low-dose of 2ME2 (0.5 micromol/L) for 0 h, 48 h and 72 h.


RESULTS
Both PRDM1alpha and PRDMIbeta3 were time-dependently upregulated, and the PRDMlalpha/beta ratio was elevated from 1.461 +/- 0.033 to 2.663 +/- 0.381 (P < 0.01), 1.929 +/- 0.334 to 2.727 +/- 0.362 (P < 0.05), 1.471 +/- 0.012 to 4.367 +/- 0.243 (P < 0.01) and 1.660 +/- 0.042 to 3.059 +/- 0.167 (P < 0.01) in NCI-H929, RPMI8226, KM3 and LP-1 cells respectively.


CONCLUSIONS
Low dose of 2ME2 can induce differentiation of myeloma cells as well as up-regulate the expression of PRDMlalpha and PRDM1beta in these cells. Elevation of PRDM1alpha/beta ratio was in a time-dependent manner and positively associated with cell differentiation and in accordance with Ying-Yang mechanism of PR-domain-containing gene family.
Jiang
Hua
H
Department of Hematology, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China.
Hou
Jian
J
D000077584:2-Methoxyestradiol; D002454:Cell Differentiation / Q000187:drug effects; D045744:Cell Line, Tumor; D004958:Estradiol / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000494:pharmacology; D006801:Humans; D009101:Multiple Myeloma / Q000235:genetics / Q000378:metabolism*; D000074462:Positive Regulatory Domain I-Binding Factor 1; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism*
D012097:Repressor Proteins; C087042:PRDM1 protein, human; D004958:Estradiol; D000077584:2-Methoxyestradiol; D000074462:Positive Regulatory Domain I-Binding Factor 1