title: The associations of LPIN1 gene expression in adipose tissue with metabolic phenotypes in the Chinese population.
abstract: The LPIN1 gene, encoding lipin-1 protein, plays critical roles in adipocyte differentiation and lipid metabolism. This study aimed to analyze the association of LPIN1 mRNA levels in human adipose tissue with metabolic phenotypes. We also examined the association of LPIN1 genetic variation with type 2 diabetes and related metabolic phenotypes in the Chinese population. The relative LPIN1 mRNA levels were measured in abdominal visceral (VAT) and subcutaneous adipose tissue (SAT) obtained from 102 nondiabetic Chinese females. Seven single-nucleotide polymorphisms (SNPs) spanning from the 5'-upstream region to the 3'-end of the LPIN1 gene were genotyped in 1,520 Chinese (760 type 2 diabetic cases and 760 controls). LPIN1 mRNA levels in VAT were negatively correlated with BMI (r = -0.21, P = 0.03), body fat percentage (r = -0.22, P = 0.02), plasma triglycerides levels (r = -0.21, P = 0.03), and plasma leptin levels (r = -0.63, P = 0.0002). LPIN1 mRNA levels were positively correlated with PPARG and ADIPOQ mRNA levels in both VAT and SAT. No single SNP of the LPIN1 gene was associated with type 2 diabetes in our population. One rare haplotype showed a significant association with type 2 diabetes (odds ratio (OR), 4.35; 95% confidence interval, 1.86-11.75; P = 4 x 10(-4)). No SNP or haplotype of the LPIN1 gene was associated with quantitative metabolic traits in the nondiabetic subjects. The results confirmed the association of LPIN1 gene expression in adipose tissue with lower adiposity and favorable metabolic profiles in the Chinese population. However, the LPIN1 gene seemed not to be a major susceptibility gene for type 2 diabetes or related metabolic phenotypes in the Chinese population.
authors: Department of Internal Medicine, National Taiwan University Hospital, Yun-Lin Branch, Yun-Lin, Taiwan.
authors: Yi-Cheng
authors: YC
authors: Chang
authors: Ling-Yin
authors: LY
authors: Chang
authors: Tien-Jyun
authors: TJ
authors: Chang
authors: Yi-Der
authors: YD
authors: Jiang
authors: Kuan-Ching
authors: KC
authors: Lee
authors: Shan-Shan
authors: SS
authors: Kuo
authors: Wei-Jei
authors: WJ
authors: Lee
authors: Lee-Ming
authors: LM
authors: Chuang
mesh_terms: D052242:Adiponectin / Q000235:genetics / Q000378:metabolism; D000273:Adipose Tissue / Q000378:metabolism*; D000328:Adult; D044466:Asian People / Q000235:genetics; D001786:Blood Glucose / Q000378:metabolism; D015992:Body Mass Index; D002681:China; D003924:Diabetes Mellitus, Type 2 / Q000235:genetics* / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D020022:Genetic Predisposition to Disease; D014644:Genetic Variation; D006239:Haplotypes; D006801:Humans; D007328:Insulin / Q000097:blood; D008055:Lipids / Q000097:blood; D008297:Male; D008875:Middle Aged; D009687:Nuclear Proteins / Q000235:genetics* / Q000378:metabolism; D009765:Obesity / Q000235:genetics* / Q000378:metabolism; D016017:Odds Ratio; D047495:PPAR gamma / Q000235:genetics / Q000378:metabolism; D010641:Phenotype; D010711:Phosphatidate Phosphatase; D020641:Polymorphism, Single Nucleotide / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D055105:Waist Circumference / Q000235:genetics
chemical_list: D052242:Adiponectin; D001786:Blood Glucose; D007328:Insulin; D008055:Lipids; D009687:Nuclear Proteins; D047495:PPAR gamma; D012333:RNA, Messenger; C485999:LPIN1 protein, human; D010711:Phosphatidate Phosphatase