The relationship between cell membrane damage and lipid peroxidation under the condition of hypoxia-reoxygenation: analysis of the mechanism using antioxidants and electron transport inhibitors.
We consecutively observed lipid peroxidation and cell membrane damage under the condition of hypoxia-reoxygenation (H/R) in cells and analyzed their mechanisms by using electron transport inhibitors and an antioxidant. In H/R experiments, lipid peroxidation and cell membrane damage were observed during the hypoxia phase. In the reoxygenation phase, lipid peroxidation stopped, while cell membrane damage did not. An antioxidant, n-acetylcystein (NAC), and potassium cyanide (KCN) inhibited lipid peroxidation and cell membrane damage, while rotenone did not inhibit either of them. Although antimycin A did not inhibit lipid peroxidation, it inhibited cell membrane damage during the hypoxia phase but not during the reoxygenation phase. These results suggested that lipid peroxidation can affect cell membrane damage as a trigger during the hypoxia phase and the generation of oxidative stress can vary depending on the inhibition locations in the electron transport system.
Yajima
Daisuke
D
Department of Legal Medicine, Graduate School of Medicine, Chiba University, Japan. yajima.d@faculty.chiba-u.jp
Motani
Hisako
H
Hayakawa
Mutsumi
M
Sato
Yayoi
Y
Sato
Kaoru
K
Iwase
Hirotaro
H
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D016923:Cell Death; D015687:Cell Hypoxia; D002462:Cell Membrane / Q000378:metabolism*; D002463:Cell Membrane Permeability / Q000187:drug effects / Q000502:physiology*; D002478:Cells, Cultured; D004579:Electron Transport / Q000187:drug effects / Q000502:physiology*; D005456:Fluorescent Dyes; D015227:Lipid Peroxidation / Q000187:drug effects / Q000502:physiology*; D051379:Mice; D010100:Oxygen / Q000502:physiology*; D017382:Reactive Oxygen Species / Q000378:metabolism; D015427:Reperfusion Injury / Q000378:metabolism; D013997:Time Factors
D000975:Antioxidants; D005456:Fluorescent Dyes; D017382:Reactive Oxygen Species; D010100:Oxygen