Biodistribution of canine leucocytes labelled with technetium-99m stannous fluoride colloid in whole blood and their ability to localise to sites of induced inflammation.
This study assessed the biodistribution of autologous leucocytes radiolabelled with technetium-99m stannous fluoride colloid (99mTcSnC) for detection of foci of induced inflammation in dogs. Venous blood was collected from seven healthy dogs and incubated with 99mTcSnC for 1h at room temperature. Radiolabelled samples were injected intravenously (IV) and the dogs were scanned using a gamma camera. Another seven healthy dogs were injected intradermally with tumour necrosis factor alpha and then IV with 99mTcSnC radiolabelled autologous blood 3h later before being scanned. The radiolabelled leucocytes localised to sites of inflammation by 30 min post-injection. IV injection of autologous leucocytes radiolabelled with 99mTcSnC appears to be a sensitive method for localisation of induced foci of inflammation in dogs.
Abushhiwa
Mohamed H
MH
Faculty of Veterinary Science, The University of Melbourne, Werribee, Victoria 3030, Australia. m.abushhiwa@pgrad.unimelb.edu.au
Salehi
Nouria S
NS
Lichtenstein
Meir
M
Lording
Peter M
PM
Finnin
Peter J
PJ
Whitton
Robert C
RC
Charles
Jennifer A
JA
Parry
Bruce W
BW
D000818:Animals; D004283:Dog Diseases / Q000175:diagnosis / Q000000981:diagnostic imaging* / Q000378:metabolism*; D004285:Dogs; D005260:Female; D007249:Inflammation / Q000175:diagnosis / Q000000981:diagnostic imaging / Q000378:metabolism / Q000662:veterinary*; D007275:Injections, Intravenous / Q000662:veterinary; D007962:Leukocytes / Q000737:chemistry / Q000378:metabolism*; D008297:Male; D011877:Radionuclide Imaging; D017556:Technetium Compounds; D014018:Tissue Distribution; D014409:Tumor Necrosis Factor-alpha / Q000008:administration & dosage
D017556:Technetium Compounds; D014409:Tumor Necrosis Factor-alpha