Glucosyl hesperidin prevents influenza a virus replication in vitro by inhibition of viral sialidase.
Hesperidin, a flavonoid obtained from citrus fruits, is known to have multiple biological activities and antimicrobial activities for human viruses; however, hesperidin has very low solubility in water and the target molecule of hesperidin for influenza virus remains unknown. A water-soluble derivative of hesperidin, glucosyl hesperidin (GH), which was synthesized by regioselective transglycosylation with cyclodextrin glucanotransferase, has been reported to have biological activities that are as or stronger than those of hesperidin. To determine the inhibitory effect of GH on influenza A virus (IAV) infection, Madin-Darby canine kidney (MDCK) cells were treated with GH before, at the same time as, and after IAV inoculation. GH treatment before IAV inoculation had no effect on virus replication, whereas, treatment with GH at the same time as or after IAV inoculation induced distinct reduction in IAV replication. Inhibition analysis of GH against two surface glycoprotein spikes of IAV revealed that GH prevents IAV replication by inhibition of viral sialidase activity that is involved in the entry and release stages on IAV infection but not by receptor binding inhibition. GH had no cytotoxic effects on MDCK cells in a dose range of 0-25 mM. Our results provide useful information for the development of novel sialidase inhibitors for influenza prevention.
Saha
Repon Kumer
RK
Department of Biochemistry, School of Pharmaceutical Sciences, University of Shizuoka.
Takahashi
Tadanobu
T
Suzuki
Takashi
T
D000818:Animals; D000998:Antiviral Agents / Q000737:chemistry / Q000494:pharmacology*; D002460:Cell Line; D003588:Cytopathogenic Effect, Viral; D004285:Dogs; D004305:Dose-Response Relationship, Drug; D005960:Glucosides / Q000737:chemistry / Q000494:pharmacology*; D006385:Hemagglutination Inhibition Tests; D006569:Hesperidin / Q000031:analogs & derivatives* / Q000737:chemistry / Q000494:pharmacology; D006801:Humans; D009980:Influenza A virus / Q000187:drug effects* / Q000201:enzymology / Q000502:physiology; D015394:Molecular Structure; D009439:Neuraminidase / Q000037:antagonists & inhibitors*; D013997:Time Factors; D014779:Virus Replication / Q000187:drug effects*; D000509:alpha-Fetoproteins / Q000494:pharmacology
D000998:Antiviral Agents; D005960:Glucosides; D000509:alpha-Fetoproteins; C487382:glucosyl hesperidin; D006569:Hesperidin; D009439:Neuraminidase