The effect of anticoagulants on the quality and biological efficacy of platelet-rich plasma.
OBJECTIVE
This study investigated the effect of anticoagulants on platelet-rich plasma (PRP) quality to determine the appropriate anticoagulants for PRP production.


METHODS
This study was carried out at the Plastic Surgery Hospital of Peking Union Medical College. The microstructure of platelets collected with heparin, citrate, acid citrate dextrose (ACD) and citrate-theophylline-adenosine-dipyridamole (CTAD) was observed. The extent of spontaneous activation of platelets was detected by measuring sP-selectin in plasma. The amount of TGF-beta1 released from PRP and the effect of PRP on cell proliferation were also studied.


RESULTS
ACD and CTAD were superior to heparin and citrate in maintaining the integrity of platelet structures and preventing the platelet spontaneous activation. ACD-PRP and CTAD-PRP released more TGF-beta1 and significantly enhanced the proliferation of human marrow stromal cells compared to heparin-PRP and citrate-PRP.


CONCLUSIONS
The PRP quality was closely related to the type of anticoagulants. ACD and CTAD are appropriate anticoagulants for PRP production.
Lei
Hua
H
Department Six of Plastic Surgery Hospital, Peking Union Medical College, China Academy of Medical Sciences, No.33 BaDaChu Road, Shijingshan District, Beijing, 100144, China.
Gui
Lai
L
Xiao
Ran
R
D000241:Adenosine / Q000494:pharmacology; D000293:Adolescent; D000328:Adult; D000925:Anticoagulants / Q000494:pharmacology*; D001792:Blood Platelets / Q000187:drug effects / Q000648:ultrastructure; D001854:Bone Marrow Cells / Q000166:cytology / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D019343:Citric Acid / Q000494:pharmacology; D004176:Dipyridamole / Q000494:pharmacology; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005947:Glucose / Q000031:analogs & derivatives / Q000494:pharmacology; D006493:Heparin / Q000494:pharmacology; D006801:Humans; D008297:Male; D046529:Microscopy, Electron, Transmission; D019007:P-Selectin / Q000097:blood; D053657:Platelet-Rich Plasma / Q000187:drug effects* / Q000378:metabolism; D017154:Stromal Cells / Q000166:cytology / Q000187:drug effects; D013806:Theophylline / Q000494:pharmacology; D053773:Transforming Growth Factor beta1 / Q000097:blood*; D055815:Young Adult
D000925:Anticoagulants; D019007:P-Selectin; D053773:Transforming Growth Factor beta1; C002113:acid citrate dextrose; D019343:Citric Acid; D004176:Dipyridamole; D006493:Heparin; D013806:Theophylline; D005947:Glucose; D000241:Adenosine