Spheroid array of fetal mouse liver cells constructed on a PEG-gel micropatterned surface: upregulation of hepatic functions by co-culture with nonparenchymal liver cells.
A spheroid array of fetal mouse liver cells, which comprise various immature cells, was constructed on a PEG-gel micropatterned surface and its hepatic activity and degree of differentiation induction were significantly upregulated by co-culture with nonparenchymal liver cells as feeder-cells.
Kojima
Ryota
R
Graduate School of Pure and Applied Science, University of Tsukuba, Tsukuba, Ibaraki 305-8573, Japan.
Yoshimoto
Keitaro
K
Takahashi
Emiko
E
Ichino
Masahiro
M
Miyoshi
Hirotoshi
H
Nagasaki
Yukio
Y
D000818:Animals; D002417:Cattle; D002454:Cell Differentiation; D018920:Coculture Techniques; D019388:Cytochrome P-450 CYP1A2 / Q000378:metabolism; D005782:Gels; D022781:Hepatocytes / Q000166:cytology / Q000378:metabolism*; D008099:Liver / Q000166:cytology*; D051379:Mice; D046228:Microarray Analysis / Q000379:methods*; D011092:Polyethylene Glycols / Q000737:chemistry*; D018874:Spheroids, Cellular / Q000166:cytology* / Q000378:metabolism; D013499:Surface Properties
D005782:Gels; D011092:Polyethylene Glycols; D019388:Cytochrome P-450 CYP1A2