Cyclin A and Cyclin E expression in resected non-small cell lung cancer stage I-IIIA.
BACKGROUND
Lung cancer is the leading cause of cancer death in the majority of developed countries. Cyclin E regulates the the G(1)-S phase transition of the cell cycle. Cyclin A increases during the S- and G(2)-phases, and is a regulator of the transition to mitosis.The aim of this study was to evaluate the prognostic significance of cyclin A and cyclin E expression in primary, resected stage I-IIIA non-small cell lung cancer (NSCLC).


METHODS
The expression of cyclin A and E was investigated in the paraffin-embedded tumor tissue of 71 patients (53 men and 18 women; age 59.27+/-8.50 years), using a monoclonal antibodies to cyclin A and to cyclin E.


RESULTS
Forty-seven out of 71 (66%) tumor tissue specimens were positive for cyclin A and twenty-six (37%) were positive for cyclin E. In the majority of cases, nuclear staining was apparent. Cyclin A and cyclin E expression was significantly higher in squamous cell carcinoma than in adenocarcinoma (cyclin A: Chi(2) Yates'a 4.6; p=0.032; cyclin E: Chi(2) Yates'a 5.12: p=0.023). The prognostic value of cyclin A and E expression was examinated in all patients and in patients with squamous cell lung cancer and adenocarcinoma and separately for every stage, but no correlations were found.


CONCLUSIONS
No prognostic value of cyclin A and E expression was found in NSCLC, but significantly higher cyclin A and E expression was found in squamous cell carcinomas than in adenocarcinomas.
Kosacka
Monika
M
Department of Pulmonology and Lung Cancer, Silesian Piasts University of Medicine in Wroclaw, ul. Grabiszynska 105, 53-439 Wroclaw, Poland. mokka113@hotmail.com
Piesiak
Pawel
P
Porebska
Irena
I
Korzeniewska
Anna
A
Dyla
Tomasz
T
Jankowska
Renata
R
D000230:Adenocarcinoma / Q000378:metabolism / Q000473:pathology / Q000601:surgery; D000368:Aged; D000911:Antibodies, Monoclonal / Q000494:pharmacology; D014408:Biomarkers, Tumor / Q000378:metabolism; D002289:Carcinoma, Non-Small-Cell Lung / Q000378:metabolism* / Q000473:pathology* / Q000601:surgery; D002294:Carcinoma, Squamous Cell / Q000378:metabolism / Q000473:pathology / Q000601:surgery; D019925:Cyclin A / Q000276:immunology / Q000378:metabolism*; D019927:Cyclin E / Q000276:immunology / Q000378:metabolism*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008175:Lung Neoplasms / Q000378:metabolism* / Q000473:pathology* / Q000601:surgery; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D011379:Prognosis
D000911:Antibodies, Monoclonal; D014408:Biomarkers, Tumor; D019925:Cyclin A; D019927:Cyclin E