[Metabolic features of continuous cell lines of mosquitoes].
The metabolism of a continuous Ae. albopictus cell line (clone C6/36) was studied relative to the consumption of an environmental component by mammalian cells of the continuous lines Vero(B) and BNK-21 under steady-state cultivation conditions. The Ae. albopictus cells were found to utilize the amino acid glycine and on glycolysis they elaborated not more than (10...20)% of acid products (lactic acid) neutralized by sodium biocarbonate, suggesting that there were also other ways of using glucose. These features lead to the conclusion that the mosquito cells form an extracellular matrix. The findings were used to certify the culture of cells, to substantiate the composition of a cultivation medium, and to optimize the latter.
Kibireva
E V
EV
Lymar'
V T
VT
Pashchenko
Iu I
IuI
Khamitov
R A
RA
D000330:Aedes / Q000166:cytology* / Q000378:metabolism*; D000818:Animals; D018929:Cell Culture Techniques; D002460:Cell Line / Q000378:metabolism*; D005998:Glycine / Q000378:metabolism; D006019:Glycolysis; D019344:Lactic Acid / Q000378:metabolism
D019344:Lactic Acid; D005998:Glycine