title: Stimulation of glucose uptake by triterpenoids from Weigela subsessilis.
abstract: Four ursane-type triterpenoids, corosolic acid (1), ilekudinol B (2), ursolic acid (3) and pomolic acid (4), were isolated from an EtOAc-soluble extract of the leaves of Weigela subsessilis. These bioactive compounds were evaluated for their glucose uptake activity and produced moderate to strong enhancement both in basal- and insulin-stimulated L6 muscle cells. In particular, corosolic acid exhibited the most potent activity, increasing uptake by basal- and insulin-stimulated myotubes by 2.63- and 3.33-fold, respectively; ilekudinol B produced 1.6- and 2.9-fold, ursolic acid produced 1.84- and 2.64-fold, and pomolic acid produced 1.6- and 2.8-fold increases. No cytotoxicities were observed for corosolic acid, ursolic acid and ilekudinol B in myoblasts, while pomolic acid at doses of 25 and 50 microm reduced cell viability by 19% and 21.8% upon 24 h treatment and by 48.6% and 54.1% upon 48 h treatment, respectively. These results suggest that ursane-type triterpenoids from W. subsessilis might enhance glucose uptake by acting as insulin mimics and as insulin sensitizers and that they could be useful as nontoxic diabetes treatment agents.
authors: Department of Food and Nutrition, Chungnam National University, 220 Gung-dong, Yuseong-gu, Daejeon 305-764, Republic of Korea. myunglee@cnu.ac.kr
authors: Myung Sun
authors: MS
authors: Lee
authors: Phuong Thien
authors: PT
authors: Thuong
mesh_terms: D000818:Animals; D027922:Caprifoliaceae / Q000737:chemistry*; D002460:Cell Line; D002470:Cell Survival; D005947:Glucose / Q000378:metabolism*; D007328:Insulin / Q000494:pharmacology; D015394:Molecular Structure; D018485:Muscle Fibers, Skeletal / Q000187:drug effects / Q000378:metabolism; D032446:Myoblasts / Q000187:drug effects* / Q000378:metabolism; D009828:Oleanolic Acid / Q000031:analogs & derivatives / Q000494:pharmacology; D010936:Plant Extracts / Q000494:pharmacology*; D051381:Rats; D014315:Triterpenes / Q000494:pharmacology*; D000097245:Ursolic Acid
chemical_list: D005947:Glucose; D007328:Insulin; D009828:Oleanolic Acid; D010936:Plant Extracts; D014315:Triterpenes; D000097245:Ursolic Acid; C547257:ilekudinol B; C115535:pomolic acid; C113861:corosolic acid