Rhythms of serum melatonin in rats with acute spinal cord injury at the cervical and thoracic regions.
OBJECTIVE
To evaluate the serum melatonin levels in acute period of the spinal cord injury (SCI) caused by trauma in the rats.


BACKGROUND
Traumatic SCI induces many types of physiological and pathological damage, including hormonal level variations.


METHODS
Forty male Sprague-Dawley rats were divided randomly into four groups. In the control group, neither laminectomy nor SCI were performed; only a large laminectomy was performed without SCI in the sham group. In the cervical and thoracic spinal trauma groups, laminectomies at C5-6 and a T6-7 were performed, respectively, followed by clip compression of the spinal cord. Blood samples were drawn 2, 6, 12 and 24 h after the procedures and assayed immediately.


RESULTS
The levels of melatonin in the neurotrauma groups were high in the first 2 h, but at the end of sixth hour, melatonin levels increased in sham-operated group, decreased in neurotrauma groups and did not change in control group. At the 12th hour, melatonin levels continued to decrease in thoracic group significantly. At the 24th hour, the melatonin levels decreased in the cervical, control and sham groups, whereas melatonin levels increased in the thoracic group.


CONCLUSIONS
The present study revealed that (1) activation of endogen melatonin secretion of the organism starts immediately after the SCI, but it shows a great lowering trend between 2 and 6 h post-SCI, (2) the tetraplegic rats, which had complete injuries at the lower cervical spinal cord, could not produce enough melatonin secretion; on the contrary, the paraplegic rats, which had complete injury at the upper thoracic spinal cord, showed normal melatonin secretion.
Gezici
A R
AR
Department of Neurosurgery, Faculty of Medicine, Abant Izzet Baysal University, Beşkavaklar Mahallesi. Oznur Apartment, No: 16/8, Bolu 14300, Turkey. geziciar@gmail.com
Karakaş
A
A
Ergün
R
R
Gündüz
B
B
D000704:Analysis of Variance; D000818:Animals; D001794:Blood Pressure / Q000502:physiology; D002574:Cervical Vertebrae / Q000473:pathology; D004195:Disease Models, Animal; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D006339:Heart Rate / Q000502:physiology; D007796:Laminectomy / Q000379:methods; D008297:Male; D008550:Melatonin / Q000097:blood*; D010507:Periodicity; D051381:Rats; D017207:Rats, Sprague-Dawley; D013119:Spinal Cord Injuries / Q000097:blood* / Q000473:pathology / Q000503:physiopathology; D013904:Thoracic Vertebrae / Q000473:pathology; D013997:Time Factors
D008550:Melatonin