title: Cyclic alphavbeta6-targeting peptide selected from biopanning with clinical potential for head and neck squamous cell carcinoma.
abstract: BACKGROUND
  A cyclic peptide-displaying phage library was used for biopanning on oral squamous cell carcinoma (OSCC) cells to identify cancer-targeting peptides. This study was designed to characterize the receptor specificity of a candidate phage clone/peptide (phage/peptide-29) and to explore the clinical potential of this peptide.
  
  
  METHODS
  Immunofluorescent confocal microscopy, phage binding assay, and immunohistochemical studies were used to demonstrate the receptor specificity of phage/peptide-29. The effect of peptide-29 on the proliferation of OSCC cells was studied using 3-dimensional (3D) cell cultures.
  
  
  RESULTS
  Phage/peptide-29 preferentially binds integrin alphavbeta6 rather than other alphav-associated integrins. Peptide-29 significantly inhibits the proliferation of OSCC cells in 3D cell cultures. On human pathological sections, phage-29 targets oral cancer cells in a alphavbeta6-dependent manner. Besides, we showed that integrin alphavbeta6 is universally (94.7%, 36/38) expressed in all major kinds of head and neck squamous cell carcinomas (HNSCC).
  
  
  CONCLUSIONS
  Peptide-29 selected from biopanning may have clinical potential for HNSCC.
authors: Department of Otolaryngology, National Cheng Kung University Medical College and Hospital, Tainan, Taiwan.
authors: Jenn-Ren
authors: JR
authors: Hsiao
authors: Yao
authors: Y
authors: Chang
authors: Yuh-Ling
authors: YL
authors: Chen
authors: Su-Huei
authors: SH
authors: Hsieh
authors: Keng-Fu
authors: KF
authors: Hsu
authors: Chun-Fu
authors: CF
authors: Wang
authors: Sen-Tien
authors: ST
authors: Tsai
authors: Ying-Tai
authors: YT
authors: Jin
mesh_terms: D000951:Antigens, Neoplasm / Q000378:metabolism*; D002294:Carcinoma, Squamous Cell / Q000378:metabolism*; D002448:Cell Adhesion; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007150:Immunohistochemistry; D016023:Integrins / Q000378:metabolism*; D018613:Microscopy, Confocal; D009062:Mouth Neoplasms / Q000378:metabolism*; D019151:Peptide Library; D010455:Peptides / Q000378:metabolism*
chemical_list: D000951:Antigens, Neoplasm; D016023:Integrins; D019151:Peptide Library; D010455:Peptides; C073878:integrin alphavbeta6