Metabolism of acenaphthylene via 1,2-dihydroxynaphthalene and catechol by Stenotrophomonas sp. RMSK.
Stenotrophomonas sp. RMSK capable of degrading acenaphthylene as a sole source of carbon and energy was isolated from coal sample. Metabolites produced were analyzed and characterized by TLC, HPLC and mass spectrometry. Identification of naphthalene-1,8-dicarboxylic acid, 1-naphthoic acid, 1,2-dihydroxynaphthalene, salicylate and detection of key enzymes namely 1,2-dihydroxynaphthalene dioxygenase, salicylaldehyde dehydrogenase and catechol-1,2-dioxygenase in the cell free extract suggest that acenaphthylene metabolized via 1,2-dihydroxynaphthalene, salicylate and catechol. The terminal metabolite, catechol was then metabolized by catechol-1,2-dioxygenase to cis,cis-muconic acid, ultimately forming TCA cycle intermediates. Based on these studies, the proposed metabolic pathway in strain RMSK is, acenaphthylene --> naphthalene-1,8-dicarboxylic acid --> 1-naphthoic acid --> 1,2-dihydroxynaphthalene --> salicylic acid --> catechol --> cis,cis-muconic acid.
Nayak
Anand S
AS
Department of Biochemistry, Gulbarga University, Gulbarga, Karnataka 585106, India.
Veeranagouda
Yaligara
Y
Lee
Kyoung
K
Karegoudar
T B
TB
D000073:Acenaphthenes / Q000737:chemistry / Q000378:metabolism*; D001673:Biodegradation, Environmental / Q000187:drug effects; D002396:Catechols / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D003470:Culture Media; D006841:Hydrocarbons, Aromatic / Q000494:pharmacology; D009284:Naphthols / Q000378:metabolism*; D010802:Phylogeny; D021241:Spectrometry, Mass, Electrospray Ionization; D020588:Stenotrophomonas / Q000201:enzymology / Q000254:growth & development / Q000302:isolation & purification / Q000378:metabolism*
D000073:Acenaphthenes; D002396:Catechols; D003470:Culture Media; D006841:Hydrocarbons, Aromatic; D009284:Naphthols; C042553:acenaphthylene; C050642:1,2-dihydroxynaphthalene; C034221:catechol