Protease-activated receptor 2 mediates interleukin-8 and intercellular adhesion molecule-1 expression in response to Aggregatibacter actinomycetemcomitans.
BACKGROUND
We investigated the mechanisms by which extracts of Aggregatibacter actinomycetemcomitans affect the inflammatory response in gingival epithelial cells.


METHODS
Human gingival cells (Ca9-22) were cultured in bacterial extracts prepared from A. actinomycetemcomitans ATCC 29522. The cells were pretreated with protease inhibitors or transfected with small interfering RNA (siRNA) specific for protease-activated receptor 2 (PAR-2).


RESULTS
The pretreatment of cells with serine protease inhibitors significantly inhibited A. actinomycetemcomitans extract-induced expression of interleukin-8 (IL-8) and intercellular adhesion molecule-1 (ICAM-1) at both the messenger RNA and protein levels. In addition, A. actinomycetemcomitans extract-induced IL-8 and ICAM-1 expression was significantly decreased in PAR-2/siRNA-transfected cells.


CONCLUSIONS
A. actinomycetemcomitans extract-induced IL-8 and ICAM-1 expression in gingival epithelial cells is mediated by PAR-2.
Shimada
T
T
Nihon University Graduate School of Dentistry, Tokyo 101-8310, Japan.
Sugano
N
N
Ikeda
K
K
Shimada
K
K
Iizuka
T
T
Ito
K
K
D016976:Aggregatibacter actinomycetemcomitans / Q000187:drug effects / Q000201:enzymology*; D045744:Cell Line, Tumor; D004847:Epithelial Cells / Q000378:metabolism*; D005881:Gingiva / Q000166:cytology / Q000378:metabolism / Q000382:microbiology*; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000096:biosynthesis*; D016209:Interleukin-8 / Q000096:biosynthesis*; D034622:RNA Interference; D044464:Receptor, PAR-2 / Q000235:genetics / Q000502:physiology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012697:Serine Endopeptidases / Q000378:metabolism; D015842:Serine Proteinase Inhibitors / Q000494:pharmacology; D014162:Transfection; D015854:Up-Regulation
D016209:Interleukin-8; D044464:Receptor, PAR-2; D015842:Serine Proteinase Inhibitors; D018799:Intercellular Adhesion Molecule-1; D012697:Serine Endopeptidases