title: Simultaneous in vivo truncation of pectic side chains.
abstract: Despite the wide occurrence of pectin in nature only a few source materials have been used to produce commercial pectins. One of the reasons for this is that many plant species contain pectins with high levels of neutral sugar side chains or that are highly substituted with acetyl or other groups. These modifications often prevent gelation, which has been a major functional requirement of commercial pectins until recently. We have previously shown that modification of pectin is possible through heterologous expression of pectin degrading enzymes in planta. To test the effect of simultaneous modification of the two main neutral pectic side chains in pectic rhamnogalacturonan I (RGI), we constitutively expressed two different enzymes in Arabidopsis thaliana that would either modify the galactan or the arabinan side chains, or both side chains simultaneously. Our analysis showed that the simultaneous truncation of arabinan and galactan side chains is achievable and does not severely affect the growth of Arabidopsis thaliana.
authors: Department of Genetics and Biotechnology, University of Aarhus, 1871 Frederiksberg C, Denmark.
authors: Jens
authors: J
authors: Obro
authors: Bernhard
authors: B
authors: Borkhardt
authors: Jesper
authors: J
authors: Harholt
authors: Michael
authors: M
authors: Skjøt
authors: William G T
authors: WG
authors: Willats
authors: Peter
authors: P
authors: Ulvskov
mesh_terms: D017360:Arabidopsis / Q000201:enzymology / Q000235:genetics*; D005685:Galactans / Q000378:metabolism; D010368:Pectins / Q000737:chemistry / Q000378:metabolism*; D030821:Plants, Genetically Modified / Q000201:enzymology / Q000235:genetics; D011133:Polysaccharide-Lyases / Q000235:genetics / Q000378:metabolism*; D011134:Polysaccharides / Q000378:metabolism
chemical_list: D005685:Galactans; D011134:Polysaccharides; C042491:rhamnogalacturonan I; C030080:araban; D010368:Pectins; D011133:Polysaccharide-Lyases