title: Omental milky spots--highly efficient "natural filter" for screening gastric cancer stem cells.
abstract: Over the last decades, cancer stem cells (CSCs) have been identified in different solid tumors and three strategies have been employed to identify them as follows: (1) expression of cell surface markers, (2) ability to exclude fluorescent dye Hoescht 33342 in a cell population termed "side population" (SP), and (3) formation of tumorspheres in suspension culture. But these methods focus on a minor subset of tumor cells, initial CSCs are still hidden among them and genuine CSCs have not yet been isolated and purified. Here we propose a new strategy to identify and isolate gastric cancer stem cells--using omental milky spots to screen gastric cancer stem cells in peritoneal metastasis mouse models of gastric cancer. This strategy directly target cancer stem cells--which can cause a new tumor by itself. Omental milky spots and their macrophages become a highly efficient "natural filter" for screening gastric cancer stem cells.
authors: Department of General Surgery, The First Affiliated Hospital, Dalian Medical University, Dalian 116011, China.
authors: Liang
authors: L
authors: Cao
authors: Xiang
authors: X
authors: Hu
authors: Yi
authors: Y
authors: Zhang
mesh_terms: D005240:Feasibility Studies; D006801:Humans; D014411:Neoplastic Stem Cells / Q000473:pathology*; D009852:Omentum / Q000473:pathology*; D013274:Stomach Neoplasms / Q000473:pathology*