Sensitive determination of 1- and 2-naphthol in human plasma by HPLC-fluorescence detection with 4-(4,5-diphenyl-1H-imidazol-2-yl)benzoyl chloride as a labeling reagent.
A sensitive HPLC-fluorescence (FL) method with FL-labeling reagent for simultaneous determination of 1- and 2-naphthol was developed. The FL-labeling reaction of naphthols with 4-(4,5-diphenyl-1H-imidazol-2-yl)benzoyl chloride (DIB-Cl) was completed in 20 min at 40 degrees C. The separation of DIB derivatives was achieved with an ODS column and isocratic elution. The LODs of 1- and 2-naphthol were 16 fmol and 14 fmol/injection, respectively, which were 5-fold and 14-fold lower than those obtained by HPLC-FL method using the native FL of naphthols. The DIB-naphthol derivatives were stable for at least 24 h even when they were placed at room temperature. The proposed method was applied to the measurement of 1- and 2-naphthol in human plasma with sufficient accuracy (90.8-109%) and precision (< 6.3%).
Ohyama
Kaname
K
Department of Environmental and Pharmaceutical Sciences, Course of Pharmaceutical Sciences, Graduate School of Biomedical Sciences, Nagasaki University, Japan.
Kishikawa
Naoya
N
Matayoshi
Kenji
K
Adutwum
Lawrence Asamoah
LA
Wada
Mitsuhiro
M
Nakashima
Kenichiro
K
Kuroda
Naotaka
N
D000818:Animals; D001565:Benzoates / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods; D006801:Humans; D007093:Imidazoles / Q000737:chemistry*; D015394:Molecular Structure; D009281:Naphthalenes / Q000737:chemistry / Q000378:metabolism; D009284:Naphthols / Q000032:analysis*; D010949:Plasma / Q000737:chemistry*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence / Q000295:instrumentation / Q000379:methods
C114684:4-(4,5-diphenyl-1H-imidazol-2-yl)benzoyl chloride; D001565:Benzoates; D007093:Imidazoles; D009281:Naphthalenes; D009284:Naphthols; C031721:naphthalene