title: Brevibacterium massiliense sp. nov., isolated from a human ankle discharge.
abstract: Gram-positive, non-spore-forming rods, strain 5401308T, were isolated from a human ankle discharge. Based on cellular morphology and the results of biochemical testing, this strain was tentatively identified as an undescribed member of the genus Brevibacterium. The major fatty acids were anteiso-C15:0 (45.3%), anteiso-C17:0 (19.2%) and iso-C15:0 (18.3%). Phylogenetic analysis based on 16S rRNA gene sequence comparison showed that the bacterium was closely related to the type strains of Brevibacterium mcbrellneri (96.3% similarity) and Brevibacterium paucivorans (95.8%). On the basis of phenotypic data and phylogenetic inference, it is proposed that this strain represents a novel species, designated Brevibacterium massiliense sp. nov.; the type strain is 5401308T (=CSUR P26T=CIP 109422T=CCUG 53855T).
authors: Laboratoire de Bactériologie-Virologie, Hôpital de la Timone, CNRS UMR 6236, IFR48, 264 rue Saint-Pierre, 13385 Marseille, Cedex 05, France. vroux91@hotmail.com
authors: Véronique
authors: V
authors: Roux
authors: Didier
authors: D
authors: Raoult
mesh_terms: D000842:Ankle / Q000382:microbiology*; D015373:Bacterial Typing Techniques; D001951:Brevibacterium / Q000737:chemistry / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005122:Exudates and Transudates / Q000382:microbiology*; D005227:Fatty Acids / Q000032:analysis; D016908:Gram-Positive Bacterial Infections / Q000382:microbiology*; D006801:Humans; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S