Morphological studies of living cells using gold nanoparticles and dark-field optical section microscopy.
The morphologic changes of living cells under drug interactions were studied by using 80-nm gold nanoparticles and dark-field optical section microscopy. The gold nanoparticles were coated with poly (L-lysine), which attached to the membranes of various cells by way of electrostatic attractive force. A three-dimensional (3-D) morphological image was obtained by measuring the peak scattering intensities of gold nanoparticles at different focal planes. An algorithm for the reconstruction of 3-D cell morphology was presented. With the measured nanoparticle images and calculations, we show morphologic changes of lung cancer cells under the interaction of cytochalasin D drug at different times.
Lee
Chia-Wei
CW
Academia Sinica, Research Center for Applied Sciences, No. 128 Academic Road Section 2, Taipei, 11529, Taiwan.
Chen
Miin Jang
MJ
Cheng
Ji-Yen
JY
Wei
Pei-Kuen
PK
D000465:Algorithms; D002289:Carcinoma, Non-Small-Cell Lung / Q000473:pathology; D048430:Cell Shape / Q000187:drug effects; D002477:Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D002478:Cells, Cultured; D015638:Cytochalasin D / Q000494:pharmacology; D006046:Gold / Q000737:chemistry* / Q000378:metabolism / Q000633:toxicity; D006367:HeLa Cells; D006801:Humans; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D008175:Lung Neoplasms / Q000473:pathology; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000633:toxicity; D008853:Microscopy / Q000379:methods*; D020349:Surface Plasmon Resonance; D014018:Tissue Distribution / Q000187:drug effects; D014407:Tumor Cells, Cultured; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
D014409:Tumor Necrosis Factor-alpha; D015638:Cytochalasin D; D006046:Gold