title: Effects of acute exercise and long-term exercise on total Na+,K+ -ATPase content and Na+,K+ -ATPase isoform expression profile in equine muscle.
abstract: OBJECTIVE
  To investigate the effects of acute exercise and long-term training on Na(+),K(+)-ATPase content, mRNA isoforms, and protein concentration in equine muscle.
  
  
  METHODS
  6 Standardbreds.
  
  
  METHODS
  Horses performed a bout of exercise on a treadmill before and after 18 weeks of combined interval and endurance training. Muscle biopsy specimens were obtained from vastus lateralis muscle (VLM) and pectoralis descendens muscle (PDM) before and after exercise. The Na(+),K(+)-ATPase content, mRNA isoforms, and protein concentrations were determined by use of [(3)H]ouabain binding, real-time PCR assay, and western blotting, respectively.
  
  
  RESULTS
  6 Na(+),K(+)-ATPase mRNA isoforms were present in equine muscle, but only A2 and B1 proteins were detected. Exercise before training resulted in increases of mRNA isoforms A1, A2, A3, and B2 in VLM and A1 and B3 in PDM. Training increased resting values for mRNA isoforms A3 and B1 in VLM and B3 in PDM. The Na(+),K(+)-ATPase, [(3)H]ouabain binding, and proteins of mRNA A2 and B1 increased in VLM, whereas in PDM, only A2 protein increased as a result of training. After training, effects of strenuous exercise on mRNA expression were no longer detectable.
  
  
  CONCLUSIONS
  Equine muscle contained all Na(+),K(+)-ATPase mRNA isoforms, but only A2 and B1 proteins could be detected. Expression of these isoforms changed as a result of strenuous exercise and long-term training, representing an adaptive response. Determination of Na(+),K(+)-ATPase gene expression may be relevant for understanding alterations in excitability during neuromuscular diseases.
authors: Department of Pathobiology, Faculty of Veterinary Medicine, Utrecht University, Yalelaan 1, NL-3584 CL Utrecht, The Netherlands.
authors: Maarten M M
authors: MM
authors: van den Burg
authors: Karin
authors: K
authors: Eizema
authors: Ellen
authors: E
authors: de Graaf-Roelfsema
authors: Eric
authors: E
authors: van Breda
authors: Inge D
authors: ID
authors: Wijnberg
authors: Johannes H
authors: JH
authors: van der Kolk
authors: Maria E
authors: ME
authors: Everts
mesh_terms: D000818:Animals; D020869:Gene Expression Profiling / Q000662:veterinary*; D015971:Gene Expression Regulation, Enzymologic / Q000502:physiology*; D006736:Horses / Q000378:metabolism*; D007527:Isoenzymes; D008297:Male; D018482:Muscle, Skeletal / Q000201:enzymology / Q000378:metabolism; D010805:Physical Conditioning, Animal; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D000254:Sodium-Potassium-Exchanging ATPase / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D013997:Time Factors
chemical_list: D007527:Isoenzymes; D012333:RNA, Messenger; D000254:Sodium-Potassium-Exchanging ATPase