Assessment of cytotoxic and cytogenetic effects of a 1,2,5-thiadiazole derivative on CHO-K1 cells. Its application as corrosion inhibitor.
This work focuses on the possible use of phenanthro[9,10-c]-1,2,5-thiadiazole 1,1-dioxide (TDZ) as a harmless corrosion inhibitor. TDZ range-dose providing minimum adverse effects to the environment and human health, with satisfactory corrosion-inhibiting properties was evaluated. Cytotoxicity and genotoxicity of TDZ at 0.57-12.50 microM concentration range were tested by neutral red, chromosomal aberrations, mitotic index, and colony formation assays. Results showed a significant increase of chromatid-type aberrations for the highest concentration of TDZ assayed (12.50 microM). Additionally, a reduction in the proliferative rate for lower concentrations was detected by the MI assay. We concluded that TDZ should be used at concentrations lower than 1.16 microM. Corrosion assays performed showed good inhibition effect (ca. 50%) at low (0.65 microM) TDZ concentration. Consequently, our results indicated that TDZ induced a time- and dose-dependent genotoxic and cytotoxic response on CHO-K1 cells. Short assays should be complemented with long exposure tests to simulate chronic contact with TDZ since lower threshold levels may be found for shorter exposures and a wrong safety range could be determined.
Grillo
C A
CA
Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas (INIFTA, CCT La Plata-CONICET), Facultad de Ciencias Exactas, Departamento de Química, Universidad Nacional de La Plata, Casilla de Correo 16, Sucursal 4, 1900 La Plata, Argentina.
Mirífico
M V
MV
Morales
M L
ML
Reigosa
M A
MA
de Mele
M Fernández Lorenzo
MF
D000818:Animals; D016466:CHO Cells; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002869:Chromosome Aberrations / Q000187:drug effects; D002875:Chromosomes / Q000187:drug effects / Q000648:ultrastructure; D003343:Corrosion; D006224:Cricetinae; D003412:Cricetulus; D003603:Cytotoxins / Q000633:toxicity*; D004121:Dimethyl Sulfoxide; D008940:Mitotic Index; D009153:Mutagens / Q000633:toxicity*; D009499:Neutral Red; D013830:Thiadiazoles / Q000633:toxicity*; D014410:Tumor Stem Cell Assay
D003603:Cytotoxins; D009153:Mutagens; D013830:Thiadiazoles; D009499:Neutral Red; D004121:Dimethyl Sulfoxide