Modular model-based design for heterologous bioproduction in bacteria.
We review the current status of expression of heterologous systems for bioenergy and bioproduction in bacteria using a model-based approach. As an aim for synthetic biology, it requires mathematical models of genetic modules that could be characterized independently of their context. This fastens the design of metabolic circuits using a combinatorial design approach, where given pathways could be optimized for maximal bioproduction, while being nontoxic for the chassis. We show how recent characterization of genetic parts, such as promoters, RBS or sRNAs could be used to fine-tune the expression of individual genes to achieve that goal. We also present lists of enzymes that are used for bioproduction, enlarging such set of biological parts.
Landrain
Thomas E
TE
Synth-Bio group. Epigenomics Project, Université d'Evry Val d'Essonne-Genopole-CNRS UPS3201, 91034 Evry, France.
Carrera
Javier
J
Kirov
Boris
B
Rodrigo
Guillermo
G
Jaramillo
Alfonso
A
D001419:Bacteria / Q000235:genetics / Q000378:metabolism*; D001709:Biotechnology / Q000379:methods*; D004798:Enzymes / Q000235:genetics / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial; D015202:Protein Engineering / Q000379:methods
D004798:Enzymes