Investigation of biomass and lipids production with Neochloris oleoabundans in photobioreactor.
The fresh water microalga Neochloris oleoabundans was investigated for its ability to accumulate lipids and especially triacylglycerols (TAG). A systematic study was conducted, from the determination of the growth medium to its characterization in an airlift photobioreactor. Without nutrient limitation, a maximal biomass areal productivity of 16.5 g m(-2) day(-1) was found. Effects of nitrogen starvation to induce lipids accumulation was next investigated. Due to initial N. oleoabundans total lipids high content (23% of dry weight), highest productivity was obtained without mineral limitation with a maximal total lipids productivity of 3.8 g m(-2) day(-1). Regarding TAG, an almost similar productivity was found whatever the protocol was: continuous production without mineral limitation (0.5 g m(-2) day(-1)) or batch production with either sudden or progressive nitrogen deprivation (0.7 g m(-2) day(-1)). The decrease in growth rate reduces the benefit of the important lipids and TAG accumulation as obtained in nitrogen starvation (37% and 18% of dry weight, respectively).
Pruvost
J
J
GEPEA, Université de Nantes, CNRS, UMR6144, Bd de l'Université, CRTT-BP 406, 44602 Saint-Nazaire Cedex, France. jeremy.pruvost@univ-nantes.fr
Van Vooren
G
G
Cogne
G
G
Legrand
J
J
D001674:Bioelectric Energy Sources; D018533:Biomass; D019149:Bioreactors; D001709:Biotechnology / Q000379:methods; D056890:Eukaryota / Q000378:metabolism*; D007218:Industrial Microbiology / Q000379:methods; D008055:Lipids / Q000737:chemistry*; D009584:Nitrogen / Q000737:chemistry; D010858:Pigmentation; D014280:Triglycerides / Q000737:chemistry
D008055:Lipids; D014280:Triglycerides; D009584:Nitrogen