[Effect of omega-3 long-chain polyunsaturated fatty acids on the cultured oligodendrocyte precursor cells].
OBJECTIVE
To explore the effect of long-chain polyunsaturated fatty acids (LPFA) on the survival and process growth of the brain hippocampal Oligodendrocyte precursor cells (OPC).


METHODS
Two kinds of cultured neural progenitor cells isolated from adult rat hippocampus were used. After arachidonic acid (AA), eicosapentaenoic acid (EPA) or docosahexaenoic acid (DHA) treatment respectively, the activity of cells were determined by Lactate dehydrogenase (LDH) assay, and the quantitative measurements of the cell processes were done after the fluorescent immune cells staining.


RESULTS
EPA, DHA and AA showed similar trends to increase the cell numbers when the concentrations were high (50 micromol/L, P<0.05), but only EPA and DHA elongated the process of OPC significantly (P<0.05).


CONCLUSIONS
omega-3 LPFA (EPA and DHA) could enhance the survival and process growth of OPC.
Yu
Xiu-jun
XJ
Department of Neurology, the Second Hospital, Hebei Medical University, Shijiazhuang 050000, China. yuxiujun720309@163.com
Yu
Bing
B
Li
Zhen-zhong
ZZ
Guo
Li
L
D000818:Animals; D002478:Cells, Cultured; D004281:Docosahexaenoic Acids / Q000494:pharmacology*; D015118:Eicosapentaenoic Acid / Q000494:pharmacology*; D005260:Female; D009836:Oligodendroglia / Q000166:cytology* / Q000187:drug effects*; D051381:Rats; D017207:Rats, Sprague-Dawley
D004281:Docosahexaenoic Acids; D015118:Eicosapentaenoic Acid