Simultaneous decoupled detection of dopamine and oxygen using pyrolyzed carbon microarrays and fast-scan cyclic voltammetry.
Microfabricated structures utilizing pyrolyzed photoresist have been shown to be useful for monitoring electrochemical processes. These previous studies, however, were limited to constant-potential measurements and slow-scan voltammetry. The work described in this paper utilizes microfabrication processes to produce devices that enable multiple fast-scan cyclic voltammetry (FSCV) waveforms to be applied to different electrodes on a single substrate. This enabled the simultaneous, decoupled detection of dopamine and oxygen. In this paper we describe the fabrication process of these arrays and show that pyrolyzed photoresist electrodes possess surface chemistry and electrochemical properties comparable to PAN-type, T-650, carbon fiber microelectrodes using background-subtracted FSCV. The functionality of the array is discussed in terms of the degree of cross talk in response to flow injections of physiologically relevant concentrations of dopamine and oxygen. Finally, other applications of pyrolyzed photoresist microelectrode arrays are shown, including spatially resolved detection of analytes and combining FSCV with amperometry for the detection of dopamine.
Zachek
Matthew K
MK
Department of Biomedical Engineering, University of North Carolina at Chapel Hill and North Carolina State University, Raleigh, North Carolina 27695, USA.
Takmakov
Pavel
P
Moody
Benjamin
B
Wightman
R Mark
RM
McCarty
Gregory S
GS
D000818:Animals; D015374:Biosensing Techniques; D001921:Brain / Q000378:metabolism; D002244:Carbon / Q000737:chemistry*; D004298:Dopamine / Q000032:analysis*; D004563:Electrochemistry / Q000295:instrumentation*; D046228:Microarray Analysis; D008839:Microelectrodes; D010100:Oxygen / Q000032:analysis*; D051381:Rats; D013059:Spectrum Analysis, Raman
C009139:pyrolytic carbon; D002244:Carbon; D010100:Oxygen; D004298:Dopamine