Phase contrast microradiography of mouse lung using synchrotron X-ray: correlation with optical microscopy.
OBJECTIVE
The purpose of this study is to evaluate the feasibility of phase contrast X-ray microtomography and microradiography, using a polychromatic synchrotron X-ray, for analysis of the mouse lung microstructure.


METHODS
Normal mice were used for experiments. Some of the mouse lungs were prepared by the lung fixation-inflation method. The resulting sponge-like inflated lung samples were used for microtomography. The remaining mouse lungs were cut into 10 microm sections and were used for microradiography and optical microscopic correlation. The experiments on mouse lung samples were performed at the 7B2 beamline of the Pohang Light Source in Korea.


RESULTS
Phase contrast X-ray microtomography of inflated lung samples showed individual alveolar structure on 3-D reconstruction. Phase contrast microradiographs of thin lung samples showed microstructure of lung, such as alveoli and bronchioles, and were well correlated with optical microscopic images.


CONCLUSIONS
The results indicate that the phase contrast X-ray microtomography and microradiography using polychromatic synchrotron X-ray is feasible for evaluation of microstructure of the lung.
Yong
Hwan Seok
HS
Department of Radiology, Korea University Guro Hospital, 97 Guro-dong, Guro-gu, Seoul, Korea.
Kang
Eun-Young
EY
Kim
Yoon Kyung
YK
Woo
Ok Hee
OH
Shin
Bong Kyoung
BK
Oh
Chil Hwan
CH
Je
Jung Ho
JH
Han
Heon
H
Seo
Jae Seung
JS
D000818:Animals; D066298:In Vitro Techniques; D008168:Lung / Q000166:cytology* / Q000000981:diagnostic imaging*; D051379:Mice; D008853:Microscopy / Q000379:methods*; D008858:Microscopy, Phase-Contrast; D055114:X-Ray Microtomography / Q000379:methods*