title: Chitosan derivative nanocarrier: safety evaluation,antibacterial property and ascorbyl palmitate encapsulation.
abstract: A chitosan derivative, methyl ether-terminated poly(ethylene oxide)-4-methoxycinnamolyphthaloylchitosan (PCPLC) was prepared, characterized and self-assembled into nanoparticles. Encapsulation of ascorbyl palmitate (AP) into PCPLC gave 6890.98 nm particles with encapsulation efficiency of 84% at 56% drug loading. The encapsulated AP showed significant improved stability as examined by 1H NMR spectroscopy.The obtained particles displayed no short-term cytotoxicity against the human skin melanoma A-375 cell line using the MTT assay and no short-term skin irritation on human volunteers using a single topical application as patch and photopatch tests. In addition, aqueous suspension of PCPLC nanoparticles successfully inhibited the growth of Escherichia coli ATCC 25922 and Staphylococcus aureus ATCC 25923.
authors: Program in Biotechnology, Faculty of Science, Chulalongkorn University, Bangkok, Thailand.
authors: Mayura
authors: M
authors: Wittayasuporn
authors: Sirirat
authors: S
authors: Rengpipat
authors: Tanapat
authors: T
authors: Palaga
authors: Pravit
authors: P
authors: Asawanonda
authors: Nattaporn
authors: N
authors: Anumansirikul
authors: Supasorn P
authors: SP
authors: Wanichwecharungruang
mesh_terms: D000328:Adult; D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000494:pharmacology*; D000970:Antineoplastic Agents / Q000008:administration & dosage*; D001205:Ascorbic Acid / Q000008:administration & dosage / Q000031:analogs & derivatives*; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D048271:Chitosan / Q000031:analogs & derivatives* / Q000494:pharmacology*; D004337:Drug Carriers / Q000737:chemistry / Q000494:pharmacology; D004926:Escherichia coli / Q000187:drug effects; D005260:Female; D006801:Humans; D008297:Male; D008826:Microbial Sensitivity Tests; D008875:Middle Aged; D053758:Nanoparticles / Q000737:chemistry*; D023422:Skin Irritancy Tests; D013211:Staphylococcus aureus / Q000187:drug effects; D055815:Young Adult
chemical_list: D000900:Anti-Bacterial Agents; D000970:Antineoplastic Agents; D004337:Drug Carriers; D048271:Chitosan; D001205:Ascorbic Acid; C031226:6-O-palmitoylascorbic acid