No association of CTLA-4 polymorphisms with susceptibility to Behçet disease.
BACKGROUND
Cytotoxic T lymphocyte-associated antigen 4 (CTLA-4) is a key negative regulator of T lymphocytes and has been shown to be associated with a number of autoimmune diseases. The present study was performed to assess the association between CTLA-4 polymorphisms and Behçet disease (BD) in Chinese patients.


METHODS
Two hundred and twenty-eight BD patients and 207 controls were analysed for four single nucleotide polymorphisms (SNPs) (-1661A/G, -318C/T, +49G/A and CT60G/A) in the CTLA-4 gene by PCR-restriction fragment length polymorphism (RFLP) analysis. The association between SNP +49A/G and BD in Chinese population as well as other ethnic groups was analysed by meta-analysis.


RESULTS
No association could be detected between CTLA-4 SNPs or haplotypes and BD. Also, no association was observed between CTLA-4 polymorphisms and BD subgroups, stratified by clinical features. A meta-analysis showed that there was no heterogeneity between studies (p = 0.60, I(2) = 0%) and that CTLA-4 SNP +49 was not associated with BD (overall effect: Z = 0.26, p = 0.79).


CONCLUSIONS
This study and a meta-analysis failed to demonstrate any association between the tested CTLA-4 polymorphisms and BD.
Du
L
L
The First Affiliated Hospital, Chongqing Medical University, Chongqing Key Laboratory of Ophthalmology, Chongqing Eye Institute, Chongqing, PR China.
Yang
P
P
Hou
S
S
Zhou
H
H
Kijlstra
A
A
D000328:Adult; D015703:Antigens, CD / Q000235:genetics*; D001528:Behcet Syndrome / Q000235:genetics* / Q000276:immunology; D060908:CTLA-4 Antigen; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006239:Haplotypes; D006801:Humans; D008297:Male; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide; D055815:Young Adult
D015703:Antigens, CD; D060908:CTLA-4 Antigen; C556706:CTLA4 protein, human