title: Production of flavin mononucleotide by metabolically engineered yeast Candida famata.
abstract: Recombinant strains of the flavinogenic yeast Candida famata able to overproduce flavin mononucleotide (FMN) that contain FMN1 gene encoding riboflavin (RF) kinase driven by the strong constitutive promoter TEF1 (translation elongation factor 1alpha) were constructed. Transformation of these strains with the additional plasmid containing the FMN1 gene under the TEF1 promoter resulted in the 200-fold increase in the riboflavin kinase activity and 100-fold increase in FMN production as compared to the wild-type strain (last feature was found only in iron-deficient medium). Overexpression of the FMN1 gene in the mutant that has deregulated riboflavin biosynthesis pathway and high level of riboflavin production in iron-sufficient medium led to the 30-fold increase in the riboflavin kinase activity and 400-fold increase in FMN production of the resulted transformants. The obtained C. famata recombinant strains can be used for the further construction of improved FMN overproducers.
authors: Institute of Cell Biology, National Academy of Sciences of Ukraine, Drahomanov Street, 14/16, Lviv 79005, Ukraine.
authors: Valentyna Y
authors: VY
authors: Yatsyshyn
authors: Olena P
authors: OP
authors: Ishchuk
authors: Andriy Y
authors: AY
authors: Voronovsky
authors: Daria V
authors: DV
authors: Fedorovych
authors: Andriy A
authors: AA
authors: Sibirny
mesh_terms: D002175:Candida / Q000235:genetics / Q000378:metabolism*; D003001:Cloning, Molecular; D005486:Flavin Mononucleotide / Q000096:biosynthesis* / Q000235:genetics; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism*; D018628:Gene Dosage / Q000235:genetics; D007501:Iron / Q000378:metabolism; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000378:metabolism*; D011401:Promoter Regions, Genetic; D012256:Riboflavin / Q000378:metabolism
chemical_list: D005656:Fungal Proteins; D005486:Flavin Mononucleotide; D007501:Iron; D017853:Phosphotransferases (Alcohol Group Acceptor); C022532:riboflavin kinase; D012256:Riboflavin