title: [Construction and identification of replication deficient recombinant adenovirus encoding F gene of subgroup A human respiratory syncytial virus].
abstract: OBJECTIVE
  A strain of replication deficient recombinant adenvirus encoding fusion glycoprotein (F) of subgroup A human respiratory syncytial virus (RSV) was constructed and the expression of F was identified.
  
  
  METHODS
  The F gene was obtained from pGEM3zf-F with Xho I and Hind III, cloned into adenoviruse shuttle vector pShuttle-CMV,and then the resulting pShuttle-CMV/F was transformed into E. coli BJ5183/p with pAdeasy-1 to produce pre-adenoviral plasmid encoding F by homologous recombination. This resultant plasmid was linearized by digestion with Pac I and transfected into 293 packaging cells to generate FGAd-F. Finally, the expression of F protein was identified by Western Blot analysis.
  
  
  RESULTS
  FGAd/F was successfully constructed, and the expression of RSV F protein was identified by Western Blot.
  
  
  CONCLUSIONS
  We have obtained a strain of replication-defective adenovirus FGAd/F encoding RSV F protein, which can be used further to investigate its protective efficacy against RSV infection in vivo.
authors: Department of Immunology, Anhui Medical University, Hefei 230032, China.
authors: Yuan
authors: Y
authors: Yuan
authors: Jin-sheng
authors: JS
authors: He
authors: Yuan-hui
authors: YH
authors: Fu
authors: Mei
authors: M
authors: Zhang
authors: Qian
authors: Q
authors: Tang
authors: Dong-liang
authors: DL
authors: Li
authors: Wei
authors: W
authors: Wei
authors: Jian-guo
authors: JG
authors: Qu
authors: Tao
authors: T
authors: Hong
mesh_terms: D000256:Adenoviridae / Q000235:genetics*; D000257:Adenoviridae Infections / Q000235:genetics; D003001:Cloning, Molecular; D015870:Gene Expression; D005822:Genetic Vectors / Q000235:genetics / Q000378:metabolism; D006801:Humans; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism*; D018113:Respiratory Syncytial Virus, Human / Q000235:genetics; D012136:Respiratory Syncytial Viruses / Q000235:genetics; D014760:Viral Fusion Proteins / Q000235:genetics / Q000378:metabolism*; D014779:Virus Replication
chemical_list: D011993:Recombinant Fusion Proteins; D014760:Viral Fusion Proteins