Fluorescent-protein-based biosensors: modulation of energy transfer as a design principle.
Genetically-encoded biosensors based on FRET between fluorescent proteins of different hues enable quantitative measurement of intracellular enzyme activities and small molecule concentrations. (To listen to a podcast about this feature, please go to the Analytical Chemistry website at pubs.acs.org/journal/ancham.).
Campbell
Robert E
RE
Department of Chemistry, University of Alberta, Edmonton, Alberta T6G 2G2, Canada. robert.e.campbell@ualberta.ca
D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D031541:Fluorescence Resonance Energy Transfer / Q000295:instrumentation* / Q000379:methods*; D049452:Green Fluorescent Proteins / Q000737:chemistry / Q000378:metabolism*; D046210:Microfluidic Analytical Techniques
D049452:Green Fluorescent Proteins