Ultrastructural and molecular-biological features of inflammatory-destructive processes in pathology of parodontal complex in children.
In children aged 11-15. under mild and moderate stage parodontitis the ultrastracture and Citokeratines 10/13 and 14 expression in epithelial lining of oral mucosa were analyzed: 1. in gingival epithelia 2. in alveolar processes epithelia. 14 cases without sings of inflammation serve as control tissue. Total number of cases - 33. After informed consent had been obtained, simples of histological tissue specimens were collected on surgical extraction of the tooth. In the control group decision on the tooth extraction was taken for the orthodontic causes. Our data indicate that: 1. Heterogenity is typical to the oral cavity epithelium: a) Ultrastructural signs of keratinization and dissociation, with typical high activity of the terminal differentiation marker cytokeratin 10/13, predominate in the keratinocytes of gingival mucosa. b) Cells with signs of germination activity predominate in the ultrastructure of mucosa alveolar processes. Such cells express cytokeratin 14, typical to nonkeratinized epithelium. 2. Tissue architectonics as well as protein contents of cytoskeleton (judging by cytokeratine expression) are speared in the parodontal pathology in children, however in contrast to alveolar mucosa, damage to the microcirculatory vessels is more pronounced in gingival mucosa. 3. Expression of cytokeratines 10/13 and 14 may indicate the process of lysis and reparation of periodontal ligament.
Khimshiashvili
N
N
Tbilisi State Medical University, Tbilisi, Georgia.
Tsagareli
Z
Z
Shishniashvili
T
T
Gogiashvili
L
L
D000293:Adolescent; D002648:Child; D005260:Female; D005881:Gingiva / Q000378:metabolism / Q000648:ultrastructure; D006801:Humans; D053550:Keratin-10 / Q000096:biosynthesis; D053535:Keratin-13 / Q000096:biosynthesis; D053547:Keratin-14 / Q000096:biosynthesis; D008297:Male; D009061:Mouth Mucosa / Q000378:metabolism* / Q000648:ultrastructure*; D010510:Periodontal Diseases / Q000378:metabolism* / Q000473:pathology*
D053535:Keratin-13; D053547:Keratin-14; D053550:Keratin-10