title: Recombinase-mediated cassette exchange provides a versatile platform for gene targeting: knockout of miR-31b.
abstract: A series of vectors has been designed to enhance the versatility of targeted homologous recombination. Recombinase-mediated cassette exchange permits sequential targeting at any locus and improves flexibility in making user-defined mutations. Application of RMCE to delete an intronic microRNA gene is described.
authors: Temasek Life Sciences Laboratory, National University of Singapore, 117604, Republic of Singapore.
authors: Ruifen
authors: R
authors: Weng
authors: Ya-Wen
authors: YW
authors: Chen
authors: Natascha
authors: N
authors: Bushati
authors: Adam
authors: A
authors: Cliffe
authors: Stephen M
authors: SM
authors: Cohen
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D004331:Drosophila melanogaster / Q000235:genetics; D018390:Gene Targeting / Q000379:methods*; D018014:Gene Transfer Techniques; D005822:Genetic Vectors / Q000138:chemical synthesis / Q000235:genetics; D035683:MicroRNAs / Q000235:genetics*; D008954:Models, Biological; D016254:Mutagenesis, Insertional / Q000379:methods*; D045522:Recombinases / Q000502:physiology*; D011995:Recombination, Genetic
chemical_list: D035683:MicroRNAs; D045522:Recombinases