Olive oil-supplemented diet alleviates acute heat stress-induced mitochondrial ROS production in chicken skeletal muscle.
We have previously shown that avian uncoupling protein (avUCP) is downregulated on exposure to acute heat stress, stimulating mitochondrial reactive oxygen species (ROS) production and oxidative damage. In this study, we investigated whether upregulation of avUCP could attenuate oxidative damage caused by acute heat stress. Broiler chickens (Gallus gallus) were fed either a control diet or an olive oil-supplemented diet (6.7%), which has been shown to increase the expression of UCP3 in mammals, for 8 days and then exposed either to heat stress (34 degrees C, 12 h) or kept at a thermoneutral temperature (25 degrees C). Skeletal muscle mitochondrial ROS (measured as H(2)O(2)) production, avUCP expression, oxidative damage, mitochondrial membrane potential, and oxygen consumption were studied. We confirmed that heat stress increased mitochondrial ROS production and malondialdehyde levels and decreased the amount of avUCP. As expected, feeding birds an olive oil-supplemented diet increased the expression of avUCP in skeletal muscle mitochondria and decreased ROS production and oxidative damage. Studies on mitochondrial function showed that heat stress increased membrane potential in state 4, which was reversed by feeding birds an olive oil-supplemented diet, although no differences in basal proton leak were observed between control and heat-stressed groups. These results show that under heat stress, mitochondrial ROS production and olive oil-induced reduction of ROS production may occur due to changes in respiratory chain activity as well as avUCP expression in skeletal muscle mitochondria.
Mujahid
Ahmad
A
Nutritional Biochemistry of Animals, Life Sciences, Graduate School of Agricultural Science, Tohoku Univ., 1-1 Tsutsumidori-Amamiyamachi, Aoba-ku, Sendai 981-8555, Japan.
Akiba
Yukio
Y
Toyomizu
Masaaki
M
D033741:Adenine Nucleotide Translocator 1 / Q000378:metabolism; D000824:Animal Nutritional Physiological Phenomena; D000818:Animals; D030161:Avian Proteins / Q000378:metabolism; D002645:Chickens; D004042:Dietary Fats, Unsaturated / Q000008:administration & dosage / Q000378:metabolism*; D019587:Dietary Supplements; D004435:Eating; D018882:Heat Stress Disorders / Q000378:metabolism*; D006861:Hydrogen Peroxide / Q000378:metabolism; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D053078:Membrane Potential, Mitochondrial; D008931:Mitochondria, Muscle / Q000378:metabolism*; D024101:Mitochondrial Proteins / Q000378:metabolism; D000071245:Mitochondrial Uncoupling Proteins; D018482:Muscle, Skeletal / Q000378:metabolism*; D000069463:Olive Oil; D018384:Oxidative Stress; D010101:Oxygen Consumption; D010938:Plant Oils / Q000008:administration & dosage / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism*; D015854:Up-Regulation; D015430:Weight Gain
D033741:Adenine Nucleotide Translocator 1; D030161:Avian Proteins; D004042:Dietary Fats, Unsaturated; D024101:Mitochondrial Proteins; D000071245:Mitochondrial Uncoupling Proteins; D000069463:Olive Oil; D010938:Plant Oils; D012333:RNA, Messenger; D017382:Reactive Oxygen Species; C422907:mitochondrial uncoupling protein, chicken; D008315:Malondialdehyde; D006861:Hydrogen Peroxide