title: Characterization of monoclonal antibodies to Junin virus nucleocapsid protein and application to the diagnosis of hemorrhagic fever caused by South American arenaviruses.
abstract: Junin virus (JUNV), Machupo virus, Guanarito virus, Sabia virus, and Chapare virus are members of New World arenavirus clade B and are the etiological agents of viral hemorrhagic fevers that occur in South America. In this study, we produced three monoclonal antibodies (MAbs) to the recombinant nucleocapsid protein of JUNV, designated C6-9, C11-12, and E4-2. The specificity of these MAbs was examined by enzyme-linked immunosorbent assay (ELISA), indirect immunofluorescence assay, and an epitope-mapping method. Using these MAbs, we developed antigen (Ag) capture ELISA systems. We showed that by using MAb C6-9, JUNV Ag was specifically detected. On the other hand, by using MAb C11-12 or E-4-2, the Ags of all human pathogenic South American arenaviruses were detected. The combined use of these Ag capture ELISA systems in the present study may be useful for the diagnosis of acute-phase viral hemorrhagic fever due to infection by a South American arenavirus.
authors: Department of Virology 1, National Institute of Infectious Diseases, Tokyo, Japan.
authors: Mina
authors: M
authors: Nakauchi
authors: Shuetsu
authors: S
authors: Fukushi
authors: Masayuki
authors: M
authors: Saijo
authors: Tetsuya
authors: T
authors: Mizutani
authors: Agustín E
authors: AE
authors: Ure
authors: Victor
authors: V
authors: Romanowski
authors: Ichiro
authors: I
authors: Kurane
authors: Shigeru
authors: S
authors: Morikawa
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000302:isolation & purification; D000914:Antibodies, Viral / Q000302:isolation & purification; D000918:Antibody Specificity; D018050:Arenavirus / Q000276:immunology / Q000302:isolation & purification*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D018604:Epitope Mapping; D019084:Fluorescent Antibody Technique, Indirect; D006478:Hemorrhagic Fever, American / Q000175:diagnosis*; D006801:Humans; D018051:Junin virus / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D019590:Nucleocapsid Proteins / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D019590:Nucleocapsid Proteins