title: Evaluation of total oxidative and antioxidative status in pediatric patients undergoing laparoscopic surgery.
abstract: OBJECTIVE
  Reactive oxygen species (ROS) induced by several diseases can trigger oxidative stress. During laparoscopy, increased intraabdominal pressure caused by pneumoperitoneum may lead to splanchnic ischemia followed by reperfusion because of deflation. Because ischemia reperfusion creates oxidative stress, in this study, we aimed to investigate the oxidative-antioxidative status of the pediatric patients with laparoscopic surgery.
  
  
  METHODS
  The children underwent laparoscopic procedures under general anesthesia, and they were mechanically ventilated. Blood samples were obtained after induction of anesthesia, at the end of the surgery, and were centrifuged at 3000 revolutions per minute for 10 minutes to separate plasma. The plasma total antioxidant status (TAS), total oxidant status (TOS), and oxidative stress index (OSI) were determined.
  
  
  RESULTS
  Plasma TOS and OSI levels were significantly higher at the end of the surgery than after induction of anesthesia (P < .05 and P < .01, respectively). On the other hand, plasma TAS levels were lower in the end of the surgery than after induction of anesthesia (P < .05).
  
  
  CONCLUSIONS
  These results suggest that ROS are generated during the laparoscopic procedure, possibly as a result of an ischemia-reperfusion phenomenon induced by the inflation and deflation of the pneumoperitoneum, which causes oxidative stress and consume plasma antioxidants.
authors: Department of Anesthesiology and Reanimation, Faculty of Medicine, Harran University, 63100 Sanliurfa, Turkey. zbaysal2003@yahoo.com
authors: Zeynep
authors: Z
authors: Baysal
authors: Turhan
authors: T
authors: Togrul
authors: Nurten
authors: N
authors: Aksoy
authors: Mustafa
authors: M
authors: Cengiz
authors: Hakim
authors: H
authors: Celik
authors: M Emin
authors: ME
authors: Boleken
authors: Mete
authors: M
authors: Kaya
authors: Galip
authors: G
authors: Yavuz
mesh_terms: D000975:Antioxidants / Q000378:metabolism*; D002675:Child, Preschool; D017558:Elective Surgical Procedures / Q000379:methods*; D006801:Humans; D010535:Laparoscopy / Q000379:methods*; D018384:Oxidative Stress / Q000502:physiology*; D011300:Preoperative Care / Q000379:methods*; D011446:Prospective Studies; D017382:Reactive Oxygen Species / Q000097:blood*; D012307:Risk Factors
chemical_list: D000975:Antioxidants; D017382:Reactive Oxygen Species