Pol bootscanning analysis of HIV type 1 can exhibit unexpected recombinations.
In France the recommendation is to sequence the RT gene of HIV-1 isolates prior to initiation of antiretroviral therapy. The data are routinely used for molecular characterization of the viruses yielding the subtype or CRF of the isolates investigated together with the absence or presence of drug resistance mutations. In this study, we performed bootscanning analysis on the whole pol gene, in which in vitro and in vivo intersubtype recombination has been reported to occur frequently. We showed that out of 15 HIV-1 isolates, two exhibited a recombination unexpected by this routine sequencing method.
Jeannot
Anne-Cécile
AC
Laboratoire de Virologie EA2968 and IFR66, Université de Bordeaux, Bordeaux, France. anne-cecile.jeannot@viro.u-bordeaux2.fr
Reigadas
Sandrine
S
Schrive
Marie-Helene
MH
Pinson
Patricia
P
Fleury
Herve J
HJ
D000328:Adult; D024882:Drug Resistance, Viral / Q000235:genetics; D005260:Female; D005602:France; D015658:HIV Infections / Q000097:blood / Q000821:virology*; D015497:HIV-1 / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D008297:Male; D009154:Mutation; D012367:RNA, Viral / Q000032:analysis / Q000097:blood / Q000235:genetics; D011995:Recombination, Genetic; D017422:Sequence Analysis, DNA; D054302:pol Gene Products, Human Immunodeficiency Virus / Q000032:analysis / Q000235:genetics*
D012367:RNA, Viral; D054302:pol Gene Products, Human Immunodeficiency Virus