Effects of mineral trioxide aggregate on the differentiation of rat dental pulp cells.
The effect of mineral trioxide aggregate (MTA) on the odontoblast-like differentiation of pulp cells was evaluated using heat-shock protein 25 (hsp25) as a marker for odontoblast differentiation. The cells were cultured with tooth-colored MTA or calcium hydroxide-containing cement (Dycal). The effects of the materials on the pulp cells were observed using a confocal laser scanning microscope. The cells were labelled immunocytochemically using polyclonal antibodies against hsp25 and actin. The mRNA expression of hsp25 and dspp in the pulp cells at 2 days were examined by quantitative reverse transcription-polymerase chain reaction (RT-PCR). Most of the cells cultured with MTA showed an intense immunolabelling for hsp25 and the mRNA expressions of hsp25 and dspp at 2 days were higher than those cultured with Dycal. These findings indicate that MTA is an effective pulp capping material and is able to induce the differentiation of odontoblast-like cells and the formation of reparative tertiary dentin with minimum apoptosis.
Masuda-Murakami
Yoshiko
Y
Department of Cariology and Endodontology, Showa University School of Dentistry, 2-1-1 Kitasenzoku, Ohta-ku, Tokyo 145-8515, Japan. yoshik@senzoku.showa-u.ac.jp
Kobayashi
Miho
M
Wang
Xiaogu
X
Yamada
Yoshishige
Y
Kimura
Yuichi
Y
Hossain
Mossamal
M
Matsumoto
Koukichi
K
D000199:Actins / Q000378:metabolism; D017607:Aluminum Compounds / Q000494:pharmacology*; D000818:Animals; D017610:Calcium Compounds / Q000494:pharmacology*; D002126:Calcium Hydroxide / Q000494:pharmacology; D002452:Cell Count; D002454:Cell Differentiation / Q000187:drug effects*; D048430:Cell Shape / Q000187:drug effects; D002478:Cells, Cultured; D003593:Cytoplasm / Q000378:metabolism; D003782:Dental Pulp / Q000166:cytology*; D004338:Drug Combinations; D016326:Extracellular Matrix Proteins / Q000235:genetics; D015870:Gene Expression / Q000187:drug effects / Q000235:genetics; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000235:genetics; D055551:HSP27 Heat-Shock Proteins / Q000378:metabolism; D008297:Male; D008903:Minerals / Q000494:pharmacology; D010087:Oxides / Q000494:pharmacology*; D010750:Phosphoproteins / Q000235:genetics; D057872:Pulp Capping and Pulpectomy Agents / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012795:Sialoglycoproteins / Q000235:genetics; D017640:Silicates / Q000494:pharmacology*; D000099311:Dentin Sialophosphoprotein
D000199:Actins; D017607:Aluminum Compounds; D017610:Calcium Compounds; D002126:Calcium Hydroxide; D004338:Drug Combinations; D016326:Extracellular Matrix Proteins; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases; D055551:HSP27 Heat-Shock Proteins; D008903:Minerals; D010087:Oxides; D010750:Phosphoproteins; D057872:Pulp Capping and Pulpectomy Agents; D012795:Sialoglycoproteins; D017640:Silicates; D000099311:Dentin Sialophosphoprotein; C486114:Hspb1 protein, rat; C086631:mineral trioxide aggregate; C025702:Dycal