title: Time-related alterations of superoxide radical levels in diverse organs of bile duct-ligated rats.
abstract: The time-related alterations of superoxide radical measured in vivo by employing an ultrasensitive fluorescent assay in the liver, intestine, kidney and brain of rats with experimentally induced obstructive jaundice was investigated. Eighteen rats were randomly divided into Group A, rats subjected to sham operation, and Group B, rats subjected to bile duct ligation (BDL). Three rats from each group were subsequently killed at different time points post-operatively (1, 5 and 10 days). As compared to sham-operated, BDL rats showed a gradual increase with time of superoxide radical in the intestine, liver, kidney and brain: for animals sacrificed on the 1(st), 5(th) and 10(th) day the increase was 45%, 50% and 96% in the liver, 76%, 81% and 118% in the intestine, 64%, 71% and 110% in the kidney and 76%, 95% and 142% in the brain, respectively. This study provides direct evidence of an early appearance of oxidative stress in diverse organs, implying a uniform systemic response to biliary obstruction and emphasizing the need of early bile flow restoration.
authors: Department of Biology, Division of Genetics, Cell and Developmental Biology, School of Medicine, University of Patras, Greece.
authors: Konstantinos
authors: K
authors: Grintzalis
authors: Ioannis
authors: I
authors: Papapostolou
authors: Stelios F
authors: SF
authors: Assimakopoulos
authors: Adamantios
authors: A
authors: Mavrakis
authors: Konstantinos
authors: K
authors: Faropoulos
authors: Nikolaos
authors: N
authors: Karageorgos
authors: Christos
authors: C
authors: Georgiou
authors: Elisabeth
authors: E
authors: Chroni
authors: Dimitris
authors: D
authors: Konstantinou
mesh_terms: D000818:Animals; D001921:Brain / Q000378:metabolism; D003135:Common Bile Duct / Q000601:surgery*; D003997:Dicarbethoxydihydrocollidine / Q000031:analogs & derivatives; D004195:Disease Models, Animal; D005456:Fluorescent Dyes; D007413:Intestinal Mucosa / Q000378:metabolism; D041781:Jaundice, Obstructive / Q000378:metabolism*; D007668:Kidney / Q000378:metabolism; D007700:Kinetics; D008026:Ligation; D008099:Liver / Q000378:metabolism; D008297:Male; D018384:Oxidative Stress; D051381:Rats; D017208:Rats, Wistar; D013050:Spectrometry, Fluorescence; D013481:Superoxides / Q000378:metabolism*
chemical_list: D005456:Fluorescent Dyes; C514241:dihydroethidine; D013481:Superoxides; D003997:Dicarbethoxydihydrocollidine