Molecular cloning and characterization of cDNA encoding buffalo (Bubalus bubalis) cytochrome P450 aromatase in the ovary.
One of the predominant causes of poor reproduction in buffaloes is low levels of ovarian estrogens. A rate limiting enzyme in estrogen biosynthesis is cytochrome P450 aromatase (P450 AROM), the product of CYP19 gene. In the present study CYP19 cDNA was cloned and its 5'UTR was characterized by 5'RACE in granulosa cells of large follicles. CYP19 transcripts with four different 5'UTRs (206, 114, 90 and 3 bases) were found in buffalo granulosa cells of large ovarian follicles. Interestingly, a predominant aromatase transcript with short 5'UTR (3 nucleotides) was found. Further studies are required to understand the relevance of these transcripts and their translational efficiency in granulosa cells of large follicles during folliculogenesis of buffalo ovary.
Kumar
O Suneel
OS
Molecular Endocrinology Lab, Animal Biochemistry Division, National Dairy Research Institute, Karnal, India.
Rao
G Jaganmohana
GJ
Singh
Dheer
D
Sharma
M K
MK
D020121:5' Untranslated Regions; D000818:Animals; D001141:Aromatase / Q000235:genetics*; D001483:Base Sequence; D002020:Buffaloes / Q000235:genetics* / Q000378:metabolism* / Q000502:physiology; D003001:Cloning, Molecular; D017931:DNA Primers / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics; D005260:Female; D006107:Granulosa Cells / Q000201:enzymology; D008969:Molecular Sequence Data; D010053:Ovary / Q000201:enzymology*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012098:Reproduction / Q000235:genetics / Q000502:physiology; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity
D020121:5' Untranslated Regions; D017931:DNA Primers; D018076:DNA, Complementary; D012333:RNA, Messenger; D001141:Aromatase