title: [Effects of DSCAM on differentiation of rat marrow mesenchymal stem cells into neurons in vitro].
abstract: OBJECTIVE
  To study the effects of Down syndrome cellular adhesion molecule (DSCAM) on differentiation of rat marrow mesenchymal stem cells (MSCs) into neurons in vitro.
  
  
  METHODS
  MSCs from Sprague-Dawley rats were induced into neurons by baicalin. The expression of DSCAM before and after induction was evaluated by immunocytochemical staining and Western blot assay. After knockdown of DSCAM by siRNA transfection, the differentiation rate of neurons derived from MSCs was measured.
  
  
  RESULTS
  Before induction, the expression of DSCAM was not detectable in MSCs. After bFGF preinduction for 24 hrs, DSCAM was slightly expressed in MSCs (1.71+/- 0.67%). The DSCAM expression increased 6 hrs after baicalin induction (15.79+/- 4.24%), reached a peak at 3 days (53.16+/- 5.94%) and then decreased gradually. The DSCAM expression 6 days after baicalin induction (28.99+/- 6.72%) was significantly lower than that at 3 days (P<0.01). However, after DSCAM-siRNA transfection, the DSCAM expression in MSCs was significantly reduced. MSCs did not express neuron-specific beta-III-tubulin before induction. After baicalin induction for 6 hrs, 3 days and 6 days, the expression of beta-III-tubulin was 1.40+/- 0.79%, 41.59+/- 3.17% and 59.11+/- 4.76% respectively. But the beta-III-tubulin expression significantly decreased 3 and 6 days after DSCAM-siRNA transfection (28.57+/- 2.91% and 43.90+/- 12.31% respectively).
  
  
  CONCLUSIONS
  DSCAM may play an important role in MSCs differentiation into neural cells.
authors: First Affiliated Hospital, Zhengzhou University, Zhengzhou, China.
authors: Ming-Chuang
authors: MC
authors: Wang
authors: Yan-Jie
authors: YJ
authors: Jia
authors: Quan-Qing
authors: QQ
authors: Wen
authors: Wen-Juan
authors: WJ
authors: Guan
authors: Er-Yi
authors: EY
authors: Zhao
authors: Liu-Dong
authors: LD
authors: Wang
authors: Bo-Ai
authors: BA
authors: Zhang
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology*; D015815:Cell Adhesion Molecules / Q000502:physiology*; D002454:Cell Differentiation; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D009474:Neurons / Q000166:cytology*; D034741:RNA, Small Interfering / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D014162:Transfection
chemical_list: D015815:Cell Adhesion Molecules; D034741:RNA, Small Interfering