title: Localization of D-β-aspartyl residue-containing proteins in various tissues.
abstract: Prior to the emergence of life, it is believed that only l-amino acids were selected for formation of protein and that d-amino acids were eliminated on the primitive Earth. Whilst homochirality is essential for life, the occurrence of proteins containing d-beta-aspartyl (Asp) residues in various tissues from elderly subjects has been reported recently. Here, we demonstrate the presence of a d-beta-Asp-containing protein in the cardiac muscle of heart, blood vessels of the lung, chief cells of the stomach, longitudinal and circular muscle of the stomach, small intestine and large intestine. Since the d-beta-Asp residue occurs through a succinimide intermediate, this isomer may potentially be generated in proteins more easily than initially thought. Formation of the d-beta-Asp residue in proteins may be related to stress.
authors: Research Reactor Institute, Kyoto University, Kumatori, Sennan, Osaka 590-0494, Japan.
authors: Ryota
authors: R
authors: Motoie
authors: Research Reactor Institute, Kyoto University, Kumatori, Sennan, Osaka 590-0494, Japan.
authors: Noriko
authors: N
authors: Fujii
authors: Graduate School of Science, Osaka Prefecture University, Sakai, Osaka 599-8531, Japan.
authors: Shigeru
authors: S
authors: Tsunoda
authors: Research Reactor Institute, Kyoto University, Kumatori, Sennan, Osaka 590-0494, Japan.
authors: Kenji
authors: K
authors: Nagata
authors: Life Science Center, Asahi Techno Glass Corp, Funabashi, Chiba 273-0044, Japan.
authors: Tadashi
authors: T
authors: Shimo-Oka
authors: Research Reactor Institute, Kyoto University, Kumatori, Sennan, Osaka 590-0494, Japan.
authors: Tadatoshi
authors: T
authors: Kinouchi
authors: Research Reactor Institute, Kyoto University, Kumatori, Sennan, Osaka 590-0494, Japan.
authors: Norihiko
authors: N
authors: Fujii
authors: Research Reactor Institute, Kyoto University, Kumatori, Sennan, Osaka 590-0494, Japan.
authors: Takeshi
authors: T
authors: Saito
authors: Research Reactor Institute, Kyoto University, Kumatori, Sennan, Osaka 590-0494, Japan.
authors: Koji
authors: K
authors: Ono
mesh_terms: D000818:Animals; D001224:Aspartic Acid / Q000737:chemistry*; D001808:Blood Vessels / Q000378:metabolism; D005260:Female; D005753:Gastric Mucosa / Q000378:metabolism; D007150:Immunohistochemistry; D007420:Intestine, Large / Q000378:metabolism; D007421:Intestine, Small / Q000378:metabolism; D007536:Isomerism; D051379:Mice; D008809:Mice, Inbred C3H; D009206:Myocardium / Q000378:metabolism; D018384:Oxidative Stress; D011506:Proteins / Q000032:analysis / Q000737:chemistry* / Q000378:metabolism
chemical_list: D011506:Proteins; D001224:Aspartic Acid