The in vitro anti-denaturation effects induced by natural products and non-steroidal compounds in heat treated (immunogenic) bovine serum albumin is proposed as a screening assay for the detection of anti-inflammatory compounds, without the use of animals, in the early stages of the drug discovery process.
There are emerging ethical issues with regards to the use of animals in the early stages of drug discovery for anti-inflammatory and degenerative diseases from natural products using the activity-directed isolation pathways when many compounds (eg > 100) are present in the crude extract or fraction and are to be tested The above-mentioned is the main reason for proposing the use of the in vitro anti-denaturation (stabilization) effects of heat treated (immunogenic) bovine serum albumin (BSA) as an assay. Current methods used for detecting and isolating a wide range of anti-inflammatory compounds in the early stages of the drug discovery process utilize a large number of animals. When BSA is heated and is undergoing denaturation, it expresses antigens associated to Type III hypersensitive reaction and which are related to diseases such as serum sickness, glomerulonephritis, rheumatoid arthritis and systemic lupus erythematosus. Thus, the assay that is being proposed should be applicable to the discovery of drugs for treating the above mentioned diseases and others, once the compounds stabilize the denaturation process.
Williams
L A D
LA
The Natural Products Unit, Scientific Research Council, PO Box 350, Hope Gardens, Kingston 6, Jamaica, West Indies. lawrencew@src-jamaica.org
O'Connar
A
A
Latore
L
L
Dennis
O
O
Ringer
S
S
Whittaker
J A
JA
Conrad
J
J
Vogler
B
B
Rosner
H
H
Kraus
W
W
D000818:Animals; D000893:Anti-Inflammatory Agents / Q000097:blood*; D001681:Biological Assay; D002417:Cattle; D055808:Drug Discovery; D006358:Hot Temperature / Q000009:adverse effects; D007154:Immune System Diseases / Q000188:drug therapy; D066298:In Vitro Techniques; D008403:Mass Screening; D028321:Plant Preparations / Q000494:pharmacology*; D011489:Protein Denaturation / Q000187:drug effects*; D012710:Serum Albumin, Bovine / Q000032:analysis*
D000893:Anti-Inflammatory Agents; D028321:Plant Preparations; D012710:Serum Albumin, Bovine