title: Control of tight junctional sealing: roles of epidermal growth factor and prostaglandin E2.
abstract: Epithelia can adjust the permeability of the paracellular permeation route by regulating the degree of sealing of the tight junction. This is reflected by a transepithelial electrical resistance (TER) ranging from a few tenths to several thousand ohms times square centimeters, depending on the difference in composition between the fluid in the lumen and the interstitial fluid. Although teleologically sound, such correlation requires a physiological explanation. We have previously shown that urine extracts from different animal species increase the TER of Madin-Darby canine kidney (MDCK) monolayers and that these effects are mediated by epidermal growth factor (EGF) contained in the flowing intratubular fluid that eventually reaches the urine. This increase in TER is accompanied by an enhanced expression of claudin-4 (cln-4) and a decrement of cln-2. These changes are transient, peaking at approximately 16 h and returning to control values in approximately 24 h. In the present work we investigated how EGF provokes this transient response, and we found that the activation of extracellular-regulated kinases 1/2 (ERK1/2) by EGF is essential to increase TER and cln-4 content, but it does not appear to participate in cln-2 downregulation. On the other hand, prostaglandin synthesis, stimulated by EGF, functions as a negative feedback, turning off the signal initiated by EGF. Thus, PGE(2) blocks ERK1/2 by a mechanism that involves the G alpha(s) protein, adenylyl cyclase as well as protein kinase A in MDCK cells. In summary, the permeability of a given segment of the nephron depends on the expression of different claudin types, which may be modulated by EGF and prostaglandins.
authors: Department of Physiology, Biophysics and Neurosciences, Center for Research and Advanced Studies (CINVESTAV), Mexico City, Mexico.
authors: D
authors: D
authors: Flores-Benitez
authors: R
authors: R
authors: Rincon-Heredia
authors: L F
authors: LF
authors: Razgado
authors: I
authors: I
authors: Larre
authors: M
authors: M
authors: Cereijido
authors: R G
authors: RG
authors: Contreras
mesh_terms: D000818:Animals; D002460:Cell Line; D005576:Colforsin; D051545:Cyclooxygenase 1 / Q000235:genetics / Q000378:metabolism; D051546:Cyclooxygenase 2 / Q000235:genetics / Q000378:metabolism; D015232:Dinoprostone / Q000378:metabolism / Q000494:pharmacology*; D004285:Dogs; D017097:Electric Impedance; D004815:Epidermal Growth Factor / Q000378:metabolism / Q000494:pharmacology*; D004847:Epithelial Cells; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism; D005786:Gene Expression Regulation / Q000502:physiology; D006801:Humans; D018078:Receptors, Prostaglandin E / Q000235:genetics / Q000378:metabolism; D019108:Tight Junctions / Q000502:physiology*
chemical_list: D018078:Receptors, Prostaglandin E; D005576:Colforsin; D004815:Epidermal Growth Factor; D051545:Cyclooxygenase 1; D051546:Cyclooxygenase 2; D048049:Extracellular Signal-Regulated MAP Kinases; D015232:Dinoprostone