Genetic variants of C1orf10 and risk of esophageal squamous cell carcinoma in a Chinese population.
Chromosome 1 open reading frame 10 (C1orf10) is either down-regulated or absent in esophageal squamous cell carcinoma (ESCC) tissues compared to its corresponding normal counterparts, and it is involved in heat shock and ethanol response and is expected to protect esophageal epithelium from damage. In the present study, we sequenced DNA samples from 32 individuals to search for genetic variants in the promoter region, coding region, and the untranslated region of C1orf10. Genotypes were analyzed in 991 patients and 984 controls. Odds ratios (ORs) and 95% confidence intervals (CIs) were estimated by logistic regression. Luciferase assays were carried out to find the functional SNPs. Six strongly linked single nucleotide polymorphisms (SNPs) spanning a region of 7 kb, -1747G/T, -1139G/C, -1079G/A, -900G/T, Gly480Ser, and 4666G/A were identified (D'= 1, r(2 )= 1). Only one SNP -1139G/C was selected to analyze the association between C1orf10 genotypes and risk of ESCC. Subjects with the -1139CC genotype had a greater risk of developing ESCC compared with those with the -1139GG genotype (adjusted OR = 1.34; 95% CI, 1.02-1.76). There appears to be an interaction between the -1139G/C polymorphism and tobacco smoking that contributes to the risk for ESCC. However, we did not detect any obvious difference in reporter gene assay driven by each allele of C1orf10 promoter or 3' UTR. These data showed that C1orf10 haplotypes containing -1747G/T, -1139G/C, -1079G/A, -900G/T, Gly480Ser, and 4666G/A are significantly associated with susceptibility to ESCC.
Zhang
Wei
W
State Key Laboratory of Molecular Oncology, Cancer Institute and Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.
Chen
Xiabin
X
Luo
Aiping
A
Lin
Dongxin
D
Tan
Wen
W
Liu
Zhihua
Z
D000368:Aged; D044466:Asian People / Q000235:genetics; D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000556:secondary; D016022:Case-Control Studies; D004938:Esophageal Neoplasms / Q000235:genetics* / Q000473:pathology; D005260:Female; D005838:Genotype; D006239:Haplotypes / Q000235:genetics; D006801:Humans; D008297:Male; D008565:Membrane Proteins / Q000235:genetics*; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000235:genetics*; D009367:Neoplasm Staging; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D011379:Prognosis; D011401:Promoter Regions, Genetic; D012307:Risk Factors; D012907:Smoking; D020506:Untranslated Regions
C418764:CRNN protein, human; D008565:Membrane Proteins; D009363:Neoplasm Proteins; D020506:Untranslated Regions