Detecting cells on the surface of a silver electrode quartz crystal microbalance using plasma treatment and graft polymerization.
This paper utilizes a silver electrode quartz crystal microbalance (QCM) mass sensor to detect the physiology of cells. This study also investigates the plasma surface modification of silver electrode QCMs through deposition of hexamethyldisilazane (HMDSZ) films as a protection film. To improve the cell growth, this paper also performs post-treatments by surface-grafting acrylic acid (AAc), acrylamide (AAm), and oxygen plasma treatment onto the QCM electrodes. Experimental results indicate that plasma deposition is a useful technique to protect the surface of silver electrodes. This technique extends the unpeeling time of silver electrodes from 1 to 7 days. The hydrophilic silver electrode QCM surface modified by AAm exhibited a better storage time effect than other post-treatments.
Chou
Hung-Che
HC
Institute of Bioengineering, Tatung University, Taipei 104, Tawain, ROC.
Yan
Tsong-Rong
TR
Chen
Ko-Shao
KS
D000818:Animals; D015374:Biosensing Techniques / Q000295:instrumentation*; D018938:Caco-2 Cells; D002470:Cell Survival / Q000528:radiation effects; D003460:Crystallization; D004566:Electrodes; D006046:Gold / Q000737:chemistry; D006801:Humans; D008027:Light; D008264:Macrophages / Q000166:cytology* / Q000528:radiation effects; D051379:Mice; D017646:Organosilicon Compounds / Q000737:chemistry; D011108:Polymers / Q000737:chemistry*; D011791:Quartz / Q000737:chemistry*; D012834:Silver / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared; D013499:Surface Properties / Q000528:radiation effects; D013997:Time Factors
D017646:Organosilicon Compounds; D011108:Polymers; D011791:Quartz; D012834:Silver; D006046:Gold; C024548:hexamethylsilazane