Imaging the cell wall of living single yeast cells using surface-enhanced Raman spectroscopy.
The surface of a living yeast cell (Saccharomyces cerevisiae strain W303-1A) has been labeled with silver (Ag) nanoparticles that can form nanoaggregates which have been shown to have surface-enhanced Raman scattering (SERS) activity. The cell wall of a single living yeast cell has been imaged by use of a Raman microspectroscope. The SERS spectra measured from different Ag nanoaggregates were found to be different. This can be explained on the basis of detailed spectral interpretation. The SERS spectral response originates from mannoproteins which cover the outermost regions of the yeast cell wall. Analysis of SERS spectra from the cell wall and the extracted mannoproteins from the yeast has been performed for the clarification of variation in SERS spectra.
Sujith
Athiyanathil
A
Health Technology Research Center, National Institute of Advanced Industrial Science and Technology, Takamatsu, Kagawa 761-0395, Japan.
Itoh
Tamitake
T
Abe
Hiroko
H
Yoshida
Ken-ichi
K
Kiran
Manikantan S
MS
Biju
Vasudevanpillai
V
Ishikawa
Misturu
M
D000327:Adsorption; D002473:Cell Wall / Q000648:ultrastructure; D053768:Metal Nanoparticles / Q000737:chemistry; D012441:Saccharomyces cerevisiae / Q000166:cytology* / Q000648:ultrastructure; D012680:Sensitivity and Specificity; D012834:Silver / Q000737:chemistry; D013059:Spectrum Analysis, Raman / Q000295:instrumentation / Q000379:methods*; D013499:Surface Properties
D012834:Silver