title: Proteome analysis of tobacco leaves under salt stress.
abstract: The mechanisms responsible for the effects of salt stress on tobacco plants were examined by means of proteomic analysis. Tobacco plants were exposed to 0, 150, 250, 300, or 400 mM NaCl. At 150 mM NaCl or above, the plants showed a reduction in fresh weight and an increase in proline levels. Proteins extracted from the leaves of tobacco plants exposed to 150 mM NaCl were separated by 2-DE. Of 205 protein spots that were detected reproducibly in each gel, 18 were differentially expressed under NaCl treatment. Up-regulated proteins belonged to the photosynthesis category, whereas down-regulated proteins correspond to defense-related functions. Dose- and time-dependent studies showed that a stromal 70-kDa heat shock-related protein was markedly down-regulated by NaCl. Thus, down-regulation of the stromal 70-kDa heat shock protein in response to salt stress is likely the cause of failure to protect cells against salt stress of tobacco plants.
authors: National Institute of Crop Science, 2-1-18 Kannondai, Tsukuba, Japan.
authors: Roya
authors: R
authors: Razavizadeh
authors: Ali Akbar
authors: AA
authors: Ehsanpour
authors: Nagib
authors: N
authors: Ahsan
authors: Setsuko
authors: S
authors: Komatsu
mesh_terms: D004305:Dose-Response Relationship, Drug; D015536:Down-Regulation / Q000187:drug effects; D015180:Electrophoresis, Gel, Two-Dimensional; D015870:Gene Expression / Q000187:drug effects / Q000235:genetics; D018840:HSP70 Heat-Shock Proteins / Q000378:metabolism; D009953:Ornithine-Oxo-Acid Transaminase / Q000235:genetics; D047492:Peroxisome Proliferator-Activated Receptors / Q000378:metabolism; D018515:Plant Leaves / Q000033:anatomy & histology / Q000187:drug effects / Q000378:metabolism*; D010940:Plant Proteins / Q000378:metabolism; D018517:Plant Roots / Q000033:anatomy & histology / Q000187:drug effects / Q000378:metabolism; D011392:Proline / Q000378:metabolism; D020543:Proteome / Q000032:analysis / Q000378:metabolism*; D012273:Ribulose-Bisphosphate Carboxylase / Q000378:metabolism; D054712:Salinity; D020539:Sequence Analysis, Protein; D017386:Sequence Homology, Amino Acid; D012965:Sodium Chloride / Q000494:pharmacology; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013312:Stress, Physiological / Q000502:physiology*; D013482:Superoxide Dismutase / Q000378:metabolism; D013997:Time Factors; D014026:Nicotiana / Q000033:anatomy & histology / Q000187:drug effects / Q000378:metabolism*; D015854:Up-Regulation / Q000187:drug effects
chemical_list: D018840:HSP70 Heat-Shock Proteins; D047492:Peroxisome Proliferator-Activated Receptors; D010940:Plant Proteins; D020543:Proteome; D012965:Sodium Chloride; D011392:Proline; D013482:Superoxide Dismutase; D009953:Ornithine-Oxo-Acid Transaminase; D012273:Ribulose-Bisphosphate Carboxylase