Targeted heavy-ion microbeam irradiation of the embryo but not yolk in the diapause-terminated egg of the silkworm, bombyx mori, induces the somatic mutation.
Using heavy-ion microbeam, we report target irradiation of selected compartments within the diapause-terminated egg and its mutational consequences in the silkworm, Bombyx mori. On one hand, carbon-ion exposure of embryo to 0.5-6 Gy increased the somatic mutation frequency, suggesting targeted radiation effects. On the other, such increases were not observed when yolk was targeted, suggesting a lack of nontargeted bystander effect.
Furusawa
Toshiharu
T
Department of Applied Biology, Faculty of Textile Science, Kyoto Institute of Technology, Matsugasaki, Sakyo-ku, Kyoto, Japan.
Fukamoto
Kana
K
Sakashita
Tetsuya
T
Suzuki
Eiko
E
Kakizaki
Takehiko
T
Hamada
Nobuyuki
N
Funayama
Tomoo
T
Suzuki
Hiromi
H
Ishioka
Noriaki
N
Wada
Seiichi
S
Kobayashi
Yasuhiko
Y
Nagaoka
Shunji
S
D000818:Animals; D012831:Bombyx / Q000196:embryology* / Q000528:radiation effects*; D004307:Dose-Response Relationship, Radiation; D004625:Embryo, Nonmammalian / Q000502:physiology* / Q000528:radiation effects*; D020450:Heavy Ions; D009154:Mutation / Q000528:radiation effects*; D010063:Ovum / Q000502:physiology* / Q000528:radiation effects*; D011829:Radiation Dosage