Mechanical properties of the hindlimb bones of bullfrogs and cane toads in bending and torsion.
When compared with most vertebrates, frogs use a novel style of jumping locomotion powered by the hindlimbs. Hindlimb bones of frogs must withstand the potentially erratic loads associated with such saltatory locomotion. To evaluate the load bearing capacity of anuran limb bones, we used three-point bending, torsion, and hardness tests to measure the mechanical properties of the femur and tibiofibula from adults of two species that use different jumping styles: explosively jumping bullfrogs (Rana (Lithobates) catesbeiana) and cyclically hopping cane toads (Bufo (Chaunus) marinus). Yield stress and strain values for R. catesbeiana and B. marinus hindlimb bones are within the range of values previously reported for other vertebrates. However, anuran hindlimb bones generally stand out as having higher yield stresses in bending than those of closely related, nonsaltatory salamanders, highlighting the importance of considering phylogenetic context in comparisons of bone functional capacity and adaptation. Stiffness values for both frog species tested were also high, which may facilitate efficient transmission of muscular forces while jumping. Elevated stiffness may also contribute to some discrepancies between determinations of bone properties via hardness versus bending tests. In comparisons between species, B. marinus bones showed significantly higher bending yield stresses than R. catesbeiana, whereas R. catesbeiana bones showed significantly higher torsional yield stresses than B. marinus. These differences may correlate with differences in jumping style and limb anatomy between ranid and bufonid frogs, suggesting that evolutionary changes in bone mechanical properties may help to accommodate new functional demands that emerge in lineages.
Wilson
Megan P
MP
Department of Biological Sciences, Clemson University, Clemson, South Carolina, USA.
Espinoza
Nora R
NR
Shah
Sagar R
SR
Blob
Richard W
RW
D000222:Adaptation, Physiological / Q000502:physiology; D000818:Animals; D001696:Biomechanical Phenomena; D001842:Bone and Bones / Q000033:anatomy & histology / Q000502:physiology*; D002024:Bufo marinus / Q000033:anatomy & histology* / Q000502:physiology; D005269:Femur / Q000033:anatomy & histology / Q000502:physiology; D005360:Fibula / Q000033:anatomy & histology / Q000502:physiology; D005684:Gait / Q000502:physiology; D006614:Hindlimb / Q000033:anatomy & histology* / Q000502:physiology; D008124:Locomotion / Q000502:physiology*; D009068:Movement / Q000502:physiology*; D010802:Phylogeny; D011892:Rana catesbeiana / Q000033:anatomy & histology* / Q000502:physiology; D013045:Species Specificity; D013314:Stress, Mechanical; D013977:Tibia / Q000033:anatomy & histology / Q000502:physiology; D054159:Torsion, Mechanical; D014562:Caudata / Q000033:anatomy & histology / Q000502:physiology; D016474:Weight-Bearing / Q000502:physiology