Antibacterial activity of recombinant hCAP18/LL37 protein secreted from Pichia pastoris.
Human antimicrobial peptide CAP18/LL37 (hCAP18/LL37) was expressed in Pichia pastoris and its antibacterial activity was tested against pathogenic bacteria. The full length ORF of hCAP18/LL37 was cloned into the pPICZaA vector followed by integration into the genomic AOX1 gene of P. pastoris. Agar diffusion assay demonstrated that the different hCAP18/LL37 transformants showed various antibacterial activities against Staphylococcus aureus, Micrococcus luteus, and Salmonella gastroenteritis. The secreted form of hCAP18/LL37 exhibited its maximum activity after 72 h incubation with 2% methanol in MM media, not in BMM. This result suggests that the yeast secreted expression system can be used as a production tool of antimicrobial peptides for industrial or pharmaceutical application.
Kim
Soon-ja
SJ
Department of Biochemistry, College of Medicine, Chungbuk National University, Cheongju 361-763, Republic of Korea. hankyol2@hanmail.net
Quan
Renshu
R
Lee
Sung-Jin
SJ
Lee
Hak-Kyo
HK
Choi
Joong-Kook
JK
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D023181:Antimicrobial Cationic Peptides / Q000235:genetics / Q000494:pharmacology*; D054804:Cathelicidins; D003470:Culture Media / Q000737:chemistry; D006801:Humans; D008826:Microbial Sensitivity Tests / Q000379:methods; D016982:Micrococcus luteus / Q000187:drug effects*; D010843:Pichia / Q000235:genetics; D011994:Recombinant Proteins / Q000235:genetics / Q000494:pharmacology; D012475:Salmonella / Q000187:drug effects*; D013211:Staphylococcus aureus / Q000187:drug effects*
D000900:Anti-Bacterial Agents; D023181:Antimicrobial Cationic Peptides; D054804:Cathelicidins; D003470:Culture Media; D011994:Recombinant Proteins