Impact of immunization against SpyCEP during invasive disease with two streptococcal species: Streptococcus pyogenes and Streptococcus equi.
Currently there is no licensed vaccine against the human pathogen Streptococcus pyogenes. The highly conserved IL-8 cleaving S. pyogenes cell envelope proteinase SpyCEP is surface expressed and is a potential vaccine candidate. A recombinant N-terminal part of SpyCEP (CEP) was expressed and purified. AntiCEP antibodies were found to neutralize the IL-8 cleaving activity of SpyCEP. CEP-immunized mice had reduced bacterial dissemination from focal S. pyogenes intramuscular infection and intranasal infection. We also identified a functional SpyCEP-homolog protease SeCEP, expressed by the equine pathogen Streptococcus equi, which was able to cleave both human and equine IL-8. CEP-immunized mice also demonstrated reduced bacterial dissemination from S. equi intramuscular infection. Therefore immunization against SpyCEP may provide protection against other streptococci species with homologous proteases.
Turner
Claire E
CE
Department of Infectious Diseases & Immunity, Hammersmith Hospital, Du Cane Road, London, W12 0NN, United Kingdom.
Kurupati
Prathiba
P
Wiles
Siouxsie
S
Edwards
Robert J
RJ
Sriskandan
Shiranee
S
D000595:Amino Acid Sequence; D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology; D000942:Antigens, Bacterial / Q000276:immunology*; D015169:Colony Count, Microbial; D008099:Liver / Q000382:microbiology; D051379:Mice; D008969:Molecular Sequence Data; D009500:Neutralization Tests; D010447:Peptide Hydrolases / Q000276:immunology*; D016415:Sequence Alignment; D013154:Spleen / Q000382:microbiology; D013290:Streptococcal Infections / Q000517:prevention & control* / Q000628:therapy*; D022541:Streptococcal Vaccines / Q000276:immunology*; D018502:Streptococcus equi / Q000276:immunology*; D013297:Streptococcus pyogenes / Q000276:immunology*; D014614:Vaccines, Synthetic / Q000276:immunology
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D022541:Streptococcal Vaccines; D014614:Vaccines, Synthetic; D010447:Peptide Hydrolases; C527349:SpyCEP protein, Streptococcus pyogenes