title: Exercise effects on tumorigenesis in a p53-deficient mouse model of breast cancer.
abstract: OBJECTIVE
  Physically active women have a reduced risk of breast cancer, but the dose of activity necessary and the role of energy balance and other potential mechanisms have not been fully explored in animal models. We examined treadmill and wheel running effects on mammary tumorigenesis and biomarkers in p53-deficient (p53(+/-)):MMTV-Wnt-1 transgenic mice.
  
  
  METHODS
  Female mice (9 wk old) were randomly assigned to the following groups in experiment 1: treadmill exercise 5 d x wk(-1), 45 min x d(-1), 5% grade at 20 m x min(-1), approximately 0.90 km x d(-1) (TREX1, n = 20) or at 24 m x min(-1), approximately 1.08 km x d(-1) (TREX2, n = 21); or a nonexercise control (CON-TREX, n = 22). In experiment 2, mice were randomly assigned to voluntary wheel running (WHL, n = 21, 2.46 +/- 1.11 km x d(-1) (mean +/- SD)) or to a nonexercise control (CON-WHL, n = 22). Body composition was measured at approximately 9 wk and serum insulin-like growth factor 1 (IGF-1) at two to three monthly time points beginning at approximately 9 wk on study. Mice were sacrificed when tumors reached 1.5 cm, mice became moribund, or there was only one mouse per treatment group remaining.
  
  
  RESULTS
  TREX1 (24 wk) and TREX2 (21 wk) had shorter median survival times than CON-TREX (34 wk; P < 0.01), whereas those of WHL and CON-WHL were similar (23 vs 24 wk; P = 0.32). TREX2 had increased multiplicity of mammary gland carcinomas compared with CON-TREX; WHL had a higher tumor incidence than CON-WHL. All exercising animals were lighter than their respective controls, and WHL had lower body fat than CON-WHL (P < 0.01). There was no difference in IGF-1 between groups (P > 0.05).
  
  
  CONCLUSIONS
  Despite beneficial or no effects on body weight, body fat, or IGF-1, exercise had detrimental effects on tumorigenesis in this p53-deficient mouse model of spontaneous mammary cancer.
authors: University of Wisconsin, Madison, WI, USA. lhcolbert@education.wisc.edu
authors: Lisa H
authors: LH
authors: Colbert
authors: Kim C
authors: KC
authors: Westerlind
authors: Susan N
authors: SN
authors: Perkins
authors: Diana C
authors: DC
authors: Haines
authors: David
authors: D
authors: Berrigan
authors: Lawrence A
authors: LA
authors: Donehower
authors: Robin
authors: R
authors: Fuchs-Young
authors: Stephen D
authors: SD
authors: Hursting
mesh_terms: D000818:Animals; D001823:Body Composition; D001943:Breast Neoplasms / Q000235:genetics; D015197:Carcinogenicity Tests; D002868:Chromosomal Proteins, Non-Histone; D004268:DNA-Binding Proteins; D005260:Female; D007334:Insulin-Like Growth Factor I / Q000032:analysis; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics*; D008325:Mammary Neoplasms, Experimental / Q000235:genetics*; D051379:Mice; D023421:Models, Animal; D008969:Molecular Sequence Data; D010805:Physical Conditioning, Animal / Q000502:physiology*; D011897:Random Allocation; D016019:Survival Analysis; D000071857:Tumor Suppressor p53-Binding Protein 1
chemical_list: D002868:Chromosomal Proteins, Non-Histone; D004268:DNA-Binding Proteins; D047908:Intracellular Signaling Peptides and Proteins; C498302:Trp53bp1 protein, mouse; D000071857:Tumor Suppressor p53-Binding Protein 1; D007334:Insulin-Like Growth Factor I