title: Classification of FABP isoforms and tissues based on quantitative evaluation of transcript levels of these isoforms in various rat tissues.
abstract: In mammals, 10 isoforms of fatty acid-binding protein (FABP) are expressed in various tissues. To understand the role of multiple FABP isoforms, we have quantitatively examined the transcript levels of individual FABP isoforms in each of various tissues by Northern blotting using synthesized RNAs corresponding to the mRNA of each isoform as external standards. As a result, absolute transcript levels of individual FABP isoforms expressed in each tissue were successfully determined. The 10 FABP isoforms were classified into three categories: (1) isoforms FABP7 and 12 were not markedly expressed in any tissue examined; (2) isoforms showing certain transcript levels in multiple tissues; and (3) isoforms FABP6, 8, and 9, expressed at certain levels in one particular tissue. Based on the expression profiles of the isoforms, individual tissues were also classified into three groups: (1) tissues in which high-level expression of FABP isoforms was not observed, (2) tissues in which multiple FABP isoforms were expressed at certain levels, and (3) tissues in which a single FABP isoform was dominantly expressed at a certain level. These results give a better understanding of the meaning of the presence of multiple FABP isoforms in mammals.
authors: Institute for Genome Research, University of Tokushima, Kuramotocho-3, Tokushima, 770-8503, Japan.
authors: Takenori
authors: T
authors: Yamamoto
authors: Atsushi
authors: A
authors: Yamamoto
authors: Masahiro
authors: M
authors: Watanabe
authors: Taisuke
authors: T
authors: Matsuo
authors: Naoshi
authors: N
authors: Yamazaki
authors: Masatoshi
authors: M
authors: Kataoka
authors: Hiroshi
authors: H
authors: Terada
authors: Yasuo
authors: Y
authors: Shinohara
mesh_terms: D000818:Animals; D015342:DNA Probes / Q000378:metabolism; D018076:DNA, Complementary / Q000235:genetics; D050556:Fatty Acid-Binding Proteins / Q000145:classification* / Q000235:genetics* / Q000378:metabolism; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D009928:Organ Specificity / Q000235:genetics*; D020033:Protein Isoforms / Q000145:classification / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats
chemical_list: D015342:DNA Probes; D018076:DNA, Complementary; D050556:Fatty Acid-Binding Proteins; D020033:Protein Isoforms; D012333:RNA, Messenger