Comparative proteome analysis of splenic lymphocytes in senescence-accelerated mice.
BACKGROUND
Senescence-accelerated mice (SAM) are commonly used as animal models for studying aging. To date, aging-related proteomes in the livers and brains of SAMP8 mice have been reported; however, spleen of SAM has not yet been used as material for studying the aging-related proteome despite its extremely important role in the progress of aging or aging-related diseases.


OBJECTIVE
To identify proteins associated with aging or aging-related diseases in SAM.


METHODS
Two-dimensional gel electrophoresis (2DE) was used to compare the proteomes of splenic lymphocytes from two strains of SAM (SAMP8 and SAMR1). Differentially expressed spots whose expression altered over twofold (p < 0.01) were identified by LC-MS/MS and database search.


RESULTS
Identification results showed that 18 differentially expressed protein spots represented 14 different proteins known to be involved in aging- or aging disease-related pathways, i.e., annexin I, calgranulin B, transaldolase, isocitrate dehydrogenase, and an unnamed protein product were involved in oxidative stress and cell defense, phosphoglycerate mutase I, aldose reductase, carbonic anhydrase I and carbonic anhydrase II were involved in glycolytic energy metabolism and homeostasis, and macrophage capping protein and high mobility group box-1 protein were involved in infection and DNA damage.


CONCLUSIONS
2DE combined with MS is effective for comparative proteome analysis of splenic lymphocytes of SAM to identify proteins closely involved in aging or aging-related diseases, especially immune diseases, and further functional studies of these proteins in SAM may throw some light on the study of biomarkers for aging or aging-related diseases.
Luo
Yuanming
Y
National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.
Li
Yaojun
Y
Lin
Chentao
C
Ma
Hong
H
Zhang
Jindan
J
Wu
Shuzhen
S
Wang
Xiaorong
X
Gao
Youhe
Y
Liu
Yanxin
Y
Zheng
Dexian
D
D000375:Aging / Q000235:genetics / Q000378:metabolism*; D000595:Amino Acid Sequence; D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D015153:Blotting, Western; D015180:Electrophoresis, Gel, Two-Dimensional; D008214:Lymphocytes / Q000378:metabolism*; D051379:Mice; D023421:Models, Animal; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000235:genetics / Q000302:isolation & purification; D020543:Proteome / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D013045:Species Specificity; D013154:Spleen / Q000166:cytology / Q000378:metabolism; D053719:Tandem Mass Spectrometry
D015415:Biomarkers; D010446:Peptide Fragments; D020543:Proteome