Detection and differentiation of normal, cancerous, and metastatic cells using nanoparticle-polymer sensor arrays.
Rapid and effective differentiation between normal and cancer cells is an important challenge for the diagnosis and treatment of tumors. Here, we describe an array-based system for identification of normal and cancer cells based on a "chemical nose/tongue" approach that exploits subtle changes in the physicochemical nature of different cell surfaces. Their differential interactions with functionalized nanoparticles are transduced through displacement of a multivalent polymer fluorophore that is quenched when bound to the particle and fluorescent after release. Using this sensing strategy we can rapidly (minutes/seconds) and effectively distinguish (i) different cell types; (ii) normal, cancerous and metastatic human breast cells; and (iii) isogenic normal, cancerous and metastatic murine epithelial cell lines.
Bajaj
Avinash
A
Department of Chemistry, University of Massachusetts, Amherst, MA 01003, USA.
Miranda
Oscar R
OR
Kim
Ik-Bum
IB
Phillips
Ronnie L
RL
Jerry
D Joseph
DJ
Bunz
Uwe H F
UH
Rotello
Vincent M
VM
D000818:Animals; D015374:Biosensing Techniques / Q000295:instrumentation*; D045744:Cell Line, Tumor; D006801:Humans; D051379:Mice; D053758:Nanoparticles / Q000737:chemistry*; D009362:Neoplasm Metastasis / Q000175:diagnosis* / Q000473:pathology*; D009369:Neoplasms / Q000175:diagnosis* / Q000473:pathology*; D011108:Polymers / Q000737:chemistry*
D011108:Polymers