Overexpression of a FAD3 desaturase increases synthesis of a polymethylene-interrupted dienoic fatty acid in seeds of Arabidopsis thaliana L.
A cDNA encoding the Arabidopsis extraplastidic linoleate desaturase (FAD3) was overexpressed in the seeds of wild-type Arabidopsis and in a mutant line that accumulates high levels of oleic acid. In the transformed wild-type plants, linolenic acid (18:3Delta9,12,15) increased from 19% to nearly 40% of total seed fatty acids, with a corresponding decrease in linoleate content (18:2Delta9,12). In the high oleate mutant, a large increase in the level of a fatty acid identified by gas-chromatography/mass-spectrometry as mangiferic acid (18:2Delta9,15) was observed. The results demonstrate that the polymethylene-interrupted dienoic fatty acid, mangiferic acid, can be produced in seed oil through the overexpression of a fatty acid n-3 desaturase.
Puttick
Debbie
D
Plant Biotechnology Institute, National Research Council Canada, 110 Gymnasium Place, Saskatoon, SK, Canada.
Dauk
Melanie
M
Lozinsky
Sharla
S
Smith
Mark A
MA
D017360:Arabidopsis / Q000235:genetics* / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000235:genetics* / Q000502:physiology; D044943:Fatty Acid Desaturases / Q000235:genetics* / Q000502:physiology; D005231:Fatty Acids, Unsaturated / Q000096:biosynthesis* / Q000737:chemistry; D015971:Gene Expression Regulation, Enzymologic; D018506:Gene Expression Regulation, Plant; D008041:Linoleic Acids / Q000096:biosynthesis; D050356:Lipid Metabolism / Q000235:genetics; D008697:Methane / Q000031:analogs & derivatives / Q000737:chemistry; D030821:Plants, Genetically Modified; D012639:Seeds / Q000235:genetics / Q000378:metabolism*; D014162:Transfection; D015854:Up-Regulation / Q000502:physiology
D029681:Arabidopsis Proteins; D005231:Fatty Acids, Unsaturated; D008041:Linoleic Acids; C504223:methylene radical; C084125:9,15-octadecadienoic acid; D044943:Fatty Acid Desaturases; C474651:fad3 protein, Arabidopsis; D008697:Methane