Neuronal activity rapidly induces transcription of the CREB-regulated microRNA-132, in vivo.
Activity-dependent changes in gene-expression are believed to underlie the molecular representation of memory. In this study, we report that in vivo activation of neurons rapidly induces the CREB-regulated microRNA miR-132. To determine if production of miR-132 is regulated by neuronal activity its expression in mouse brain was monitored by quantitative RT-PCR (RT-qPCR). Pilocarpine-induced seizures led to a robust, rapid, and transient increase in the primary transcript of miR-132 (pri-miR-132) followed by a subsequent rise in mature microRNA (miR-132). Activation of neurons in the hippocampus, olfactory bulb, and striatum by contextual fear conditioning, odor-exposure, and cocaine-injection, respectively, also increased pri-miR-132. Induction kinetics of pri-miR-132 were monitored and found to parallel those of immediate early genes, peaking at 45 min and returning to basal levels within 2 h of stimulation. Expression levels of primary and mature-miR-132 increased significantly between postnatal Days 10 and 24. We conclude that miR-132 is an activity-dependent microRNA in vivo, and may contribute to the long-lasting proteomic changes required for experience-dependent neuronal plasticity.
Nudelman
Aaron S
AS
Department of Pharmacology, University of Washington, Seattle, Washington.
DiRocco
Derek P
DP
Lambert
Talley J
TJ
Garelick
Michael G
MG
Le
Josh
J
Nathanson
Neil M
NM
Storm
Daniel R
DR
D000818:Animals; D001245:Association Learning / Q000502:physiology; D001522:Behavior, Animal / Q000502:physiology; D017362:Cyclic AMP Response Element-Binding Protein / Q000235:genetics*; D015870:Gene Expression / Q000235:genetics; D006624:Hippocampus / Q000502:physiology; D008297:Male; D051379:Mice; D035683:MicroRNAs / Q000235:genetics*; D009473:Neuronal Plasticity / Q000235:genetics*; D009474:Neurons / Q000502:physiology*; D010862:Pilocarpine; D012333:RNA, Messenger / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012640:Seizures / Q000139:chemically induced / Q000235:genetics*; D014158:Transcription, Genetic / Q000235:genetics*
D017362:Cyclic AMP Response Element-Binding Protein; D035683:MicroRNAs; D012333:RNA, Messenger; D010862:Pilocarpine