[Molecular characterization and sequence comparison of the M and S segments of Hantavirus ZJ5 strain].
OBJECTIVE
In order to understand the molecular characters of Hantavirus ZJ5 strain, its complete M and S genome were sequenced and compared with that of other hantavirus strains.


METHODS
We prepared the total RNA from ZJ5. Infected cells and the raw or purified RT-PCR product was cloned and sequenced.


RESULTS
With sequence compation, we found ZJ5 strain complete M and S segment had higher homology with SEO-type strains than other type of HV, but differential genes were 11.7%-19.2% and 6.7%-14.5% from SEOV. The phylogenetic trees constructed by complete M ind S segment showed that ZJ5 strain was located in SEOV group, and structured alone embranchment.


CONCLUSIONS
The ZJ5 strain was believed to belong to SEO-type virus,and suggest that ZJ5 strain is a new subtype S SEOV group,and structured alone embranchment.


CONCLUSIONS
The ZJ5 strain was believed to belong to SEO-type virus, and suggest that ZJ5 strain is a new subtype from other SEO viruses.
Yao
Ping-ping
PP
Zhejiang Center for Disease Prevention and Control, Hangzhou 310051, China. pingpingyao@yahoo.com.cn
Zhu
Han-ping
HP
Xu
Fang
F
Xie
Rong-hui
RH
Mei
Ling-ling
LL
Lu
Qun-ying
QY
Zhu
Zhi-yong
ZY
Deng
Xiao-zhao
XZ
Zhang
Yun
Y
D000595:Amino Acid Sequence; D000914:Antibodies, Viral / Q000235:genetics; D001483:Base Sequence; D004279:DNA, Viral / Q000032:analysis*; D030541:Databases, Genetic; D006476:Orthohantavirus / Q000235:genetics*; D006480:Hemorrhagic Fever with Renal Syndrome / Q000821:virology*; D016744:Minor Lymphocyte Stimulatory Loci / Q000235:genetics*; D008969:Molecular Sequence Data; D010802:Phylogeny; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA
D000914:Antibodies, Viral; D004279:DNA, Viral