title: Folate overproduction in Lactobacillus plantarum WCFS1 causes methotrexate resistance.
abstract: Folate overproduction can serve as a mode of resistance against the folate antagonist methotrexate in Lactobacillus plantarum WCFS1. When compared with a wild-type control strain, an engineered high folate-producing strain was found to be insensitive to methotrexate. The growth rate and the viable count of the folate-overproducing L. plantarum strain were not significantly affected by the presence of methotrexate in the growth medium.
authors: Arno
authors: A
authors: Wegkamp
authors: Willem M
authors: WM
authors: de Vos
authors: Eddy J
authors: EJ
authors: Smid
mesh_terms: D024881:Drug Resistance, Bacterial; D005492:Folic Acid / Q000378:metabolism*; D048191:Lactiplantibacillus plantarum / Q000187:drug effects* / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D008727:Methotrexate / Q000494:pharmacology*
chemical_list: D005492:Folic Acid; D008727:Methotrexate