title: Synthesis and application of photoaffinity probe containing an intact isoprenoid chain.
abstract: Two novel chemical probes each carrying an intact isoprenoid chain, a biotin tag and a benzophenone moiety were synthesized. Photoaffinity labeling of the Saccharomyces cerevisiae cell lysate revealed that these probes could selectively trap some proteins, and proteins with molecular weight of approximately 70 KDa appeared as a major band upon Streptavidin blot analysis.
authors: Dalian Institute of Chemical Physics, CAS, Dalian 116023, PR China.
authors: Lingdong
authors: L
authors: Li
authors: Wei
authors: W
authors: Tang
authors: Zongbao Kent
authors: ZK
authors: Zhao
mesh_terms: D019920:Photoaffinity Labels / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology; D011106:Polyisoprenyl Phosphates / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry / Q000378:metabolism; D013729:Terpenes / Q000138:chemical synthesis* / Q000737:chemistry; D014466:Ultraviolet Rays
chemical_list: D019920:Photoaffinity Labels; D011106:Polyisoprenyl Phosphates; D029701:Saccharomyces cerevisiae Proteins; D013729:Terpenes; C011082:polyprenyl monophosphate