Folate overproduction in Lactobacillus plantarum WCFS1 causes methotrexate resistance.
Folate overproduction can serve as a mode of resistance against the folate antagonist methotrexate in Lactobacillus plantarum WCFS1. When compared with a wild-type control strain, an engineered high folate-producing strain was found to be insensitive to methotrexate. The growth rate and the viable count of the folate-overproducing L. plantarum strain were not significantly affected by the presence of methotrexate in the growth medium.
Wegkamp
Arno
A
de Vos
Willem M
WM
Smid
Eddy J
EJ
D024881:Drug Resistance, Bacterial; D005492:Folic Acid / Q000378:metabolism*; D048191:Lactiplantibacillus plantarum / Q000187:drug effects* / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D008727:Methotrexate / Q000494:pharmacology*
D005492:Folic Acid; D008727:Methotrexate