APRIL knockdown suppresses migration and invasion of human colon carcinoma cells.
OBJECTIVE
The purpose of this study was to investigate the function of a proliferation-inducing ligand (APRIL) gene among the metastatic colorectal cancer (CRC) disease.


METHODS
Cell adhesion, cell migration and invasion behavior were detected after knockdown of APRIL expression in the colon carcinoma cell line, SW480 by RNAi and the rescue experiments were performed with recombinant human APRIL (rhAPRIL) in vitro.


RESULTS
This original study indicated that knockdown of APRIL expression strongly inhibited colon carcinoma cell adhesion, cell migration and invasion in vitro. By contrast, reconstitution of APRIL expression with rhAPRIL in these APRILi cells, prominently restores CRC cell migration and invasion.


CONCLUSIONS
These results strongly suggest that APRIL play an important role in the tumor development of the invasive or migratory behavior of CRC cells and may be a useful therapeutic target in the malignant colon carcinomas.
Ding
Weifeng
W
Medical Laboratory Center, Affiliated Hospital of Nantong University, No. 20 Road Xisi, Nantong, Jiangsu, PR China.
Wang
Jinchun
J
Sun
Baolan
B
Ju
Shaoqing
S
Yuan
Hongxiang
H
Wang
Xing
X
Wang
Yueguo
Y
Wang
Huimin
H
D015153:Blotting, Western; D045744:Cell Line, Tumor; D015179:Colorectal Neoplasms / Q000473:pathology*; D055785:Gene Knockdown Techniques; D006801:Humans; D009361:Neoplasm Invasiveness / Q000235:genetics*; D009362:Neoplasm Metastasis / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014162:Transfection; D053300:Tumor Necrosis Factor Ligand Superfamily Member 13 / Q000235:genetics*
D012333:RNA, Messenger; D053300:Tumor Necrosis Factor Ligand Superfamily Member 13