title: [Experimental investigation on protective effect of dexamethasone therapy to traumatic brain injury in rats].
abstract: OBJECTIVE
  To explore the effects of dexamethasone on nuclear factor-KappaB (NF-KappaB) expression in brain tissue after traumatic brain injury (TBI).
  
  
  METHODS
  Forty rats were randomly divided into two groups: dexamethasone treatment and no treatment, and severe brain injury was produced by gas percussion in both groups. At 0, 6, 24, 72 and 120 hours after injury, 5 rats of each group were executed and the histopathological changes in brain tissue in rats were observed by hematoxylin-eosin (HE) stain. The expression of NF-KappaB in brain tissue of rats was detected by immunohistochemical method.
  
  
  RESULTS
  NF-KappaB expression was significantly up-regulated at 6 hours in brain tissue of rats after TBI (P<0.05), reaching the highest level at 24 hours (P<0.01). It showed a tendency to lower, but was still high at 120 hours after TBI (P<0.05 or P<0.01). After treatment with dexamethasone, NF-KappaB level was lowered at 6, 24 and 72 hours (all P<0.01).
  
  
  CONCLUSIONS
  NF-KappaB expression is up-regulated in brain tissue in early period after TBI, and keeps on a high level, thus inducing inflammatory response to produce secondary injury to brain tissue. Dexamethasone shows protective effects by regulating the levels of NF-KappaB and prevents secondary injury which is caused by the inflammatory cytokines in rat brain tissue after TBI.
authors: Department of Emergency, West China Hospital, Sichuan University, Chengdu 610041, Sichuan, China.
authors: Xiao-dong
authors: XD
authors: Du
authors: Guo-ping
authors: GP
authors: Li
authors: Lei
authors: L
authors: Ye
authors: Peng
authors: P
authors: Liu
authors: Yu
authors: Y
authors: Cao
mesh_terms: D000818:Animals; D001921:Brain / Q000378:metabolism / Q000473:pathology; D001930:Brain Injuries / Q000378:metabolism* / Q000473:pathology; D003907:Dexamethasone / Q000494:pharmacology*; D004195:Disease Models, Animal; D005260:Female; D008297:Male; D016328:NF-kappa B / Q000378:metabolism*; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar
chemical_list: D016328:NF-kappa B; D003907:Dexamethasone