In vitro culture and differentiation of osteoblasts from human umbilical cord blood.
It is well accepted that human umbilical cord blood (UCB) is a source of mesenchymal stem cells (MSCs) which are able to differentiate into different cell phenotypes such as osteoblasts, chondrocytes, adipocytes, myocytes, cardiomyocytes and neurons. The aim of this study was to isolate MSCs from human UCB to determine their osteogenic potential by using different kinds of osteogenic medium. Eventually, only those MSCs cultured in osteogenic media enriched with vitamin D(2) and FGF9, were positive for osteocalcin by RT-PCR. All these cells were positive for alizarin red, alkaline phosphatase and Von Kossa. The results obtained from RT-PCR have confirmed that osteogenesis is complete by expression of the osteocalcin marker. In conclusion, vitamin D(2), at least in vitro, may replace vitamin D(3) as an osteogenic stimulator factor for MSC differentiation.
Toai
Tran Cong
TC
Department of Histo-pathology, Embryology, Genetics and Biotechnology for Tissue Transplants, Pham Ngoc Thach Medical University, Ho Chi Minh City, Vietnam.
Thao
Huynh Duy
HD
Thao
Nguyen Phuong
NP
Gargiulo
Ciro
C
Ngoc
Phan Kim
PK
Van
Pham Hung
PH
Strong
D Michael
DM
D015415:Biomarkers / Q000378:metabolism; D018929:Cell Culture Techniques / Q000379:methods*; D002454:Cell Differentiation / Q000187:drug effects; D048430:Cell Shape / Q000187:drug effects; D002478:Cells, Cultured; D003470:Culture Media / Q000494:pharmacology; D004872:Ergocalciferols / Q000494:pharmacology; D005312:Fetal Blood / Q000166:cytology*; D051525:Fibroblast Growth Factor 9 / Q000494:pharmacology; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000166:cytology / Q000187:drug effects; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D010006:Osteoblasts / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D015675:Osteocalcin / Q000235:genetics / Q000378:metabolism; D010012:Osteogenesis / Q000187:drug effects; D020133:Reverse Transcriptase Polymerase Chain Reaction
D015415:Biomarkers; D003470:Culture Media; D004872:Ergocalciferols; D051525:Fibroblast Growth Factor 9; D015675:Osteocalcin