Protein composition of oil bodies from mature Brassica napus seeds.
Seed oil bodies (OBs) are intracellular particles storing lipids as food or biofuel reserves in oleaginous plants. Since Brassica napus OBs could be easily contaminated with protein bodies and/or myrosin cells, they must be purified step by step using floatation technique in order to remove non-specifically trapped proteins. An exhaustive description of the protein composition of rapeseed OBs from two double-zero varieties was achieved by a combination of proteomic and genomic tools. Genomic analysis led to the identification of sequences coding for major seed oil body proteins, including 19 oleosins, 5 steroleosins and 9 caleosins. Most of these proteins were also identified through proteomic analysis and displayed a high level of sequence conservation with their Arabidopsis thaliana counterparts. Two rapeseed oleosin orthologs appeared acetylated on their N-terminal alanine residue and both caleosins and steroleosins displayed a low level of phosphorylation.
Jolivet
Pascale
P
NRA, UMR 206, Chimie Biologique, Thiverval-Grignon, France. jolivet@grignon.inra.fr
Boulard
Céline
C
Bellamy
Annick
A
Larré
Colette
C
Barre
Marion
M
Rogniaux
Hélène
H
d'Andréa
Sabine
S
Chardot
Thierry
T
Nesi
Nathalie
N
D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000235:genetics; D029688:Brassica napus / Q000737:chemistry* / Q000235:genetics; D029741:Brassica rapa / Q000737:chemistry / Q000235:genetics; D002135:Calcium-Binding Proteins / Q000032:analysis; D015151:Immunoblotting; D002479:Inclusion Bodies / Q000737:chemistry*; D008969:Molecular Sequence Data; D010802:Phylogeny; D010940:Plant Proteins / Q000032:analysis; D011499:Protein Processing, Post-Translational; D055314:Seed Storage Proteins / Q000032:analysis* / Q000737:chemistry / Q000235:genetics; D012639:Seeds / Q000737:chemistry*; D016415:Sequence Alignment
D002135:Calcium-Binding Proteins; D010940:Plant Proteins; D055314:Seed Storage Proteins; C402836:caleosin protein, Sesamum indicum; C079356:oleosin protein, Brassica napus