Dyella soli sp. nov. and Dyella terrae sp. nov., isolated from soil.
Two novel strains isolated from soils, JS12-10(T) and JS14-6(T), were characterized using a polyphasic approach to determine their taxonomic positions. These isolates were found to be aerobic, Gram-negative, motile with one polar flagellum, non-spore-forming and rod-shaped. Phenotypic and fatty acid data supported the affiliation of JS12-10(T) and JS14-6(T) to the genus Dyella. However, chemotaxonomic data and DNA-DNA relatedness values allowed differentiation of these strains from other Dyella species with validly published names. Strains JS12-10(T) and JS14-6(T) showed the highest 16S rRNA gene sequence similarities with Dyella ginsengisoli Gsoil 3046(T) (98.4 %) and Dyella japonica XD53(T) (97.9 %), respectively, and the 16S rRNA gene sequence similarity between them was 97.1 %. DNA-DNA hybridization values between the novel isolates and strains of other recognized Dyella species were 29-38 %. Therefore, strains JS12-10(T) and JS14-6(T) represent two novel species of the genus Dyella, for which the names Dyella soli sp. nov. (type strain JS12-10(T) =KACC 12747(T) =JCM 15423(T)) and Dyella terrae sp. nov. (type strain JS14-6(T) =KACC 12748(T) =JCM 15424(T)) are proposed.
Weon
Hang-Yeon
HY
Applied Microbiology Division, National Institute of Agricultural Science and Technology, Rural Development Administration (RDA), Suwon 441-707, Republic of Korea.
Anandham
Rangasamy
R
Kim
Byung-Yong
BY
Hong
Seung-Beom
SB
Jeon
Young-Ah
YA
Kwon
Soon-Wo
SW
D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000032:analysis; D005227:Fatty Acids / Q000032:analysis; D020459:Genes, rRNA; D005838:Genotype; D007723:Korea; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010641:Phenotype; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D013045:Species Specificity; D044166:Xanthomonadaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology
D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S