title: DNA wrapping is required for DNA damage recognition in the Escherichia coli DNA nucleotide excision repair pathway.
abstract: Localized DNA melting may provide a general strategy for recognition of the wide array of chemically and structurally diverse DNA lesions repaired by the nucleotide excision repair (NER) pathway. However, it is not clear what causes such DNA melting and how it is driven. Here, we show a DNA wrapping-melting model supported by results from dynamic monitoring of the key DNA-protein and protein-protein interactions involved in the early stages of the Escherichia coli NER process. Using an analytical technique involving capillary electrophoresis coupled with laser-induced fluorescence polarization, which combines a mobility shift assay with conformational analysis, we demonstrate that DNA wrapping around UvrB, mediated by UvrA, is an early event in the damage-recognition process during E. coli NER. DNA wrapping of UvrB was confirmed by Förster resonance energy transfer and fluorescence lifetime measurements. This wrapping did not occur with readily denaturable damaged DNA substrates ("bubble" DNA), suggesting that DNA wrapping of UvrB plays an important role in the induction of DNA melting around the damage site. Analysis of DNA wrapping of mutant UvrB Y96A further suggests that a cooperative interaction between DNA wrapping of UvrA(2)B and contact of the beta-hairpin of UvrB with the bulky damage moiety may be involved in the local DNA melting at the damage site.
authors: Department of Laboratory Medicine and Pathology, University of Alberta, Edmonton, AB, Canada T6G 2G3.
authors: Hailin
authors: H
authors: Wang
authors: Meiling
authors: M
authors: Lu
authors: Moon-shong
authors: MS
authors: Tang
authors: Bennett
authors: B
authors: Van Houten
authors: J B Alexander
authors: JB
authors: Ross
authors: Michael
authors: M
authors: Weinfeld
authors: X Chris
authors: XC
authors: Le
mesh_terms: D004247:DNA / Q000737:chemistry*; D004249:DNA Damage; D004260:DNA Repair; D019075:Electrophoresis, Capillary; D004926:Escherichia coli / Q000235:genetics*; D005454:Fluorescence Polarization; D031541:Fluorescence Resonance Energy Transfer; D008958:Models, Molecular; D009690:Nucleic Acid Conformation; D014466:Ultraviolet Rays
chemical_list: D004247:DNA