Analysis of in vitro and in vivo sensitivity of oral cancer cells to methotrexate.
OBJECTIVE
The present study was directed on the assessment of the response of treatment-naive oral cancer cells to methotrexate (MTX) in vitro: and clinical response to MTX therapy.


METHODS
A pilot study of in vitro: evaluation of MTX response of oral cancer cells from 10 patients was conducted using a cell viability assay to determine the sensitivity/resistance to MTX. Quantitative in vitro: data were correlated to the clinical outcome to MTX therapy.


RESULTS
A positive correlation was observed between the effect of MTX on tumor cells in vitro: and clinical response for 7 out of 10 patients.


CONCLUSIONS
Observations from the proof-of-principle pilot study suggests that oral cancer cells have intrinsically variable response to MTX. Confirmation of these findings with a larger cohort of patients could aid in the development of individualized therapies for this class of malignancy.
Pai
R B
RB
Kidwai Memorial Institute of Oncology, Hosur Road, Bangalore 560029, India.
Lalitha
R M
RM
Pai
S B
SB
Kumaraswamy
S V
SV
Lalitha
N
N
Bhargava
M K
MK
D000328:Adult; D000368:Aged; D000964:Antimetabolites, Antineoplastic / Q000627:therapeutic use*; D002470:Cell Survival / Q000187:drug effects; D019008:Drug Resistance, Neoplasm; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D008297:Male; D008727:Methotrexate / Q000627:therapeutic use*; D008875:Middle Aged; D009062:Mouth Neoplasms / Q000188:drug therapy* / Q000473:pathology; D009367:Neoplasm Staging; D010865:Pilot Projects; D016896:Treatment Outcome
D000964:Antimetabolites, Antineoplastic; D008727:Methotrexate