title: Sulfonation and anticoagulant activity of botryosphaeran from Botryosphaeria rhodina MAMB-05 grown on fructose.
abstract: Botryosphaeran (EPS(FRU)), an exopolysaccharide of the beta-(1-->3,1-->6)-d-glucan type with 31% branching at C-6, is produced by the fungus Botryosphaeria rhodina MAMB-05 when grown on fructose as carbon source. Botryosphaeran was derivatized by sulfonation to induce anticoagulant activity. The effectiveness of the sulfonation reaction by chlorosulfonic acid in pyridine was monitored by the degree of substitution and FT-IR analysis of the sulfonated EPS(FRU) (once sulfonated, EPS(FRUSULF); and re-sulfonated, EPS(FRURESULF)). Activated partial thromboplastin time (APTT) and thrombin time (TT) tests of EPS(FRURESULF) indicated significant in vitro anticoagulant activity that was dose-dependent. EPS(FRU) did not inhibit any of the coagulation tests.
authors: Dept(o) de Física, Química e Biologia, FCT, Universidade Estadual Paulista, CEP 19060-900, Presidente Prudente - São Paulo, Brazil.
authors: Simone Ferreira
authors: SF
authors: Mendes
authors: Osvaldo
authors: O
authors: dos Santos
authors: Aneli M
authors: AM
authors: Barbosa
authors: Ana Flora D
authors: AF
authors: Vasconcelos
authors: Gabriel
authors: G
authors: Aranda-Selverio
authors: Nilson K
authors: NK
authors: Monteiro
authors: Robert F H
authors: RF
authors: Dekker
authors: Mariana
authors: M
authors: Sá Pereira
authors: Ana Maria F
authors: AM
authors: Tovar
authors: Paulo A de Souza
authors: PA
authors: Mourão
authors: Maria de Lourdes Corradi
authors: Mde L
authors: da Silva
mesh_terms: D000925:Anticoagulants / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D001203:Ascomycota / Q000254:growth & development* / Q000378:metabolism*; D001777:Blood Coagulation / Q000187:drug effects; D005632:Fructose / Q000378:metabolism*; D005936:Glucans / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology*; D006493:Heparin / Q000494:pharmacology; D006801:Humans; D017550:Spectroscopy, Fourier Transform Infrared; D013451:Sulfonic Acids / Q000378:metabolism*
chemical_list: D000925:Anticoagulants; D005936:Glucans; D013451:Sulfonic Acids; C483238:botryosphaeran; D005632:Fructose; D006493:Heparin