[Effective inactivatian test of inactivated hepatitis A vaccine using integrated cell culture/strand-specific reverse transcriptase-polymerase chain reaction].
OBJECTIVE
To establish an quick, sensitive and specific assay for effective inactivatian test of inactivated hepatitis A vaccine.


METHODS
effective inactivatian test of inactivated hepatitis A vaccine were carried out using integrated cell culture/strand-specific RT-PCR (ICC/strand-specific RT-PCR) assay compared with traditional ELISA and nest RT-PCR assay.


RESULTS
all the samples were infectious negative detecting by both ICC/ strand-specific RT-PCR and ELISA assay,while some samples appeared false positive detecting by nest RT-PCR.


CONCLUSIONS
ICC/strand-specific RT-PCR assay is a novel, rapid, sensitive and reliable method for effective inactivatian test of inactivated hepatitis A vaccine. Shorting detection period largely, this assay may be used as an alternative method for routine inactivated hepatitis A vaccines test.
Zhou
Jian
J
Chinese Academy of Medical Sciences and Peking Union Medical College, Kunming 650118, China.
Ji
Guang
G
Wen
Jia-na
JN
Li
Jia
J
Sheng
Wei
W
Guo
Zhi-quan
ZQ
Liao
Guo-yang
GY
Jiang
Shu-de
SD
Sun
Ming-bo
MB
D000818:Animals; D001709:Biotechnology / Q000379:methods*; D018929:Cell Culture Techniques; D002478:Cells, Cultured; D004797:Enzyme-Linked Immunosorbent Assay; D022362:Hepatitis A Vaccines / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D012680:Sensitivity and Specificity; D015164:Vaccines, Inactivated / Q000378:metabolism*; D014765:Viral Vaccines / Q000378:metabolism*; D014776:Virus Cultivation / Q000379:methods; D038301:Virus Inactivation
D022362:Hepatitis A Vaccines; D015164:Vaccines, Inactivated; D014765:Viral Vaccines