title: Precisely programmed and robust 2D streptavidin nanoarrays by using periodical nanometer-scale wells embedded in DNA origami assembly.
abstract: A new punched DNA origami assembly with periodic nanometer-scale wells has been successfully designed and constructed. Through the attachment of two biotins at the two edges of each well, just one streptavidin (SA) tetramer (d = 5 nm) was size-selectively captured in each 6.8 x 12 x 2.0 nm well; this allowed formation of a 28 nm-period SA nanoarray of individual molecules. The position of SA capture can be fully controlled by placement of biotins in the nanoarray well. Moreover, construction of a 2D nanoarray of individual SA tetramers through selective positioning of SA tetramers in any desired wells in a complex of such punched origami motifs is also possible. The stability of the SA captured by this fixation strategy (DNA wells and two biotin linkers) was directly compared on the same molecule with the stability of SA captured with other possible strategies that do not employ wells or two linkers. In this way, the robustness of this means of fixation was clearly established.
authors: Research Center for Advanced Science and Technology, The University of Tokyo, 4-6-1 Komaba, Meguro, Tokyo 153-8904, Japan. kuzu@mkomi.rcast.u-tokyo.ac.jp
authors: Akinori
authors: A
authors: Kuzuya
authors: Mayumi
authors: M
authors: Kimura
authors: Kentaro
authors: K
authors: Numajiri
authors: Naohiro
authors: N
authors: Koshi
authors: Toshiyuki
authors: T
authors: Ohnishi
authors: Fuminori
authors: F
authors: Okada
authors: Makoto
authors: M
authors: Komiyama
mesh_terms: D001710:Biotin / Q000737:chemistry; D004247:DNA / Q000737:chemistry*; D036103:Nanotechnology / Q000379:methods*; D040081:Protein Array Analysis / Q000295:instrumentation / Q000379:methods*; D019809:Streptavidin / Q000737:chemistry*
chemical_list: D001710:Biotin; D004247:DNA; D019809:Streptavidin