Simultaneous determination of dimethylamine, trimethylamine and trimethylamine-n-oxide in aquatic products extracts by ion chromatography with non-suppressed conductivity detection.
An ion chromatography method with non-suppressed conductivity detection was developed for the simultaneous determination of dimethylamine (DMA), trimethylamine (TMA) and trimethylamine-n-oxide (TMAO) in aquatic products. They were separated by means of cation-exchange chromatography using a 3.0 mmol/L methanesulfonic acid solution as eluent and an IonPac CS17 column (250 mm x 4 mm i.d.) as the separation column. Detection limits of dimethylamine, trimethylamine and trimethylamine-n-oxide were 0.06, 0.08 and 0.10 mg/L, respectively. The relative standard deviations (RSDs) of peak area were less than 3.53%. The recoveries were between 93.7% and 104.1%. Unlike traditional methods, this validated method is inexpensive and stable.
Li
Feng
F
College of Food Science and Engineering, Ocean University of China, No. 5, Yu Shan Road, Qingdao, Shandong Province 266003, China.
Liu
Hong-ying
HY
Xue
Chang-hu
CH
Xin
Xue-qian
XQ
Xu
Jie
J
Chang
Yao-guang
YG
Xue
Yong
Y
Yin
Li-ang
LA
D002852:Chromatography, Ion Exchange / Q000379:methods*; D004123:Dimethylamines / Q000032:analysis*; D004553:Electric Conductivity; D016014:Linear Models; D008744:Methylamines / Q000032:analysis*; D011188:Potassium / Q000737:chemistry; D000644:Quaternary Ammonium Compounds / Q000737:chemistry; D015203:Reproducibility of Results; D017747:Seafood / Q000032:analysis*; D012680:Sensitivity and Specificity; D012964:Sodium / Q000737:chemistry; D014238:Trichloroacetic Acid / Q000737:chemistry
D004123:Dimethylamines; D008744:Methylamines; D000644:Quaternary Ammonium Compounds; D014238:Trichloroacetic Acid; D012964:Sodium; C034516:dimethylamine; C005855:trimethyloxamine; C023336:trimethylamine; D011188:Potassium