title: Characterization of start-up performance and archaeal community shifts during anaerobic self-degradation of waste-activated sludge.
abstract: Successful start-up strategy for anaerobic digestion of waste-activated sludge using internal inoculum and relationship between the shift of methanogenic community and the digester performance during start-up was investigated. Combination of TS control of inoculum and batch operation during early days enabled the successful start-up operation without serious volatile fatty acid accumulation, followed by the stable continuous operation. However, the propionate degradation was rate-limiting step during the batch operation. The results of real-time quantitative polymerase chain reaction analysis suggested that there was a correlation between the population of the genus Methanosarcina and the methane production rate coupled with acetate consumption during batch operation, and the results of terminal-restriction fragment length polymorphism (T-RFLP) revealed that the increasing intensity of T-RF peaks of hydrogenotrophic methanogens was associated with a decrease in the level of C3-acids.
authors: Department of Civil and Environmental Engineering, Tohoku University, 6-6-06 Aza-Aoba, Aramaki, Aoba-ku, Sendai, Miyagi 980-8579, Japan.
authors: Takuro
authors: T
authors: Kobayashi
authors: Daisuke
authors: D
authors: Yasuda
authors: Yu-You
authors: YY
authors: Li
authors: Kengo
authors: K
authors: Kubota
authors: Hideki
authors: H
authors: Harada
authors: Han-Qing
authors: HQ
authors: Yu
mesh_terms: D000693:Anaerobiosis; D001105:Archaea / Q000235:genetics / Q000378:metabolism*; D001673:Biodegradation, Environmental; D019149:Bioreactors / Q000382:microbiology; D002999:Clone Cells; D005232:Fatty Acids, Volatile / Q000032:analysis; D008697:Methane / Q000378:metabolism; D010802:Phylogeny; D012150:Polymorphism, Restriction Fragment Length; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012722:Sewage / Q000382:microbiology*; D013997:Time Factors; D014866:Waste Products / Q000032:analysis*
chemical_list: D005232:Fatty Acids, Volatile; D012336:RNA, Ribosomal, 16S; D012722:Sewage; D014866:Waste Products; D008697:Methane