title: High-throughput sequence analysis of variants of human cytomegalovirus strains Towne and AD169.
abstract: The genomes of commonly used variants of human cytomegalovirus (HCMV) strains Towne and AD169 each contain a substantial mutation in which a region (U(L)/b') at the right end of the long unique region has been replaced by an inverted duplication of a region from the left end of the genome. Using high-throughput technology, we have sequenced HCMV strain Towne (ATCC VR-977) and confirmed the presence of two variants, one exhibiting the replacement in U(L)/b' and the other intact in this region. Both variants are mutated in genes RL13, UL1, UL40, UL130, US1 and US9. We have also sequenced a novel AD169 variant (varUC) that is intact in U(L)/b' except for a small deletion that affects genes UL144, UL142, UL141 and UL140. Like other AD169 variants, varUC is mutated in genes RL5A, RL13, UL36 and UL131A. A subpopulation of varUC contains an additional deletion affecting genes IRS1, US1 and US2.
authors: MRC Virology Unit, Institute of Virology, Church Street, Glasgow G11 5JR, UK.
authors: Amanda J
authors: AJ
authors: Bradley
authors: Department of Immunology and Microbiology, Rush University Medical Center, 1653 West Congress Parkway, Chicago, IL 60612, USA.
authors: Nell S
authors: NS
authors: Lurain
authors: Division of Pathway Medicine, University of Edinburgh Medical School, Chancellor's Building, 49 Little France Crescent, Edinburgh EH16 4SB, UK.
authors: Peter
authors: P
authors: Ghazal
authors: The Gene Pool, Ashworth Laboratories, Institute of Evolutionary Biology, King's Buildings, Edinburgh EH9 3JT, UK.
authors: Urmi
authors: U
authors: Trivedi
authors: MRC Virology Unit, Institute of Virology, Church Street, Glasgow G11 5JR, UK.
authors: Charles
authors: C
authors: Cunningham
authors: MRC Virology Unit, Institute of Virology, Church Street, Glasgow G11 5JR, UK.
authors: Katarina
authors: K
authors: Baluchova
authors: MRC Virology Unit, Institute of Virology, Church Street, Glasgow G11 5JR, UK.
authors: Derek
authors: D
authors: Gatherer
authors: Department of Medical Microbiology, Tenovus Building, School of Medicine, Cardiff University, Heath Park, Cardiff CF14 4XX, UK.
authors: Gavin W G
authors: GWG
authors: Wilkinson
authors: MRC Virology Unit, Institute of Virology, Church Street, Glasgow G11 5JR, UK.
authors: Derrick J
authors: DJ
authors: Dargan
authors: MRC Virology Unit, Institute of Virology, Church Street, Glasgow G11 5JR, UK.
authors: Andrew J
authors: AJ
authors: Davison
mesh_terms: D001483:Base Sequence; D003587:Cytomegalovirus / Q000145:classification* / Q000235:genetics*; D004279:DNA, Viral; D014644:Genetic Variation; D016679:Genome, Viral; D006801:Humans; D009154:Mutation
chemical_list: D004279:DNA, Viral