title: Estradiol-17beta and linseed meal interact to alter visceral organ mass and hormone concentrations from ovariectomized ewes.
abstract: To evaluate the estrogenic potential of secoisolariciresinol diglycoside (SDG) found in linseed meal (LSM) on visceral organ mass, IGF-I, and thyroid hormone (T(3) and T(4)) concentrations, 48 multiparous, ovariectomized ewes (54.6 +/- 1.1 kg) were used in a 3 x 4 factorial arrangement. Main effects were length of LSM feeding (0, 1, 7, or 14 d) and length of exposure to estradiol-17beta (E(2)) implant (0, 6, or 24 h prior to tissue collection). Implanting ewes with E(2) for 24h increased liver mass relative to empty body weight (EBW; g/kg EBW) compared with ewes implanted for 0 or 6 h (P <or= 0.03), whereas feeding LSM for 14 d decreased liver mass compared with ewes fed LSM for 1 or 7 d (P <or= 0.02). There was an LSM x E(2) interaction (P = 0.01) for duodenal mass (g/kg EBW), LSM, and E(2) tended (P = 0.07) to influence the stomach complex mass; however, ileal mass was not affected. Neither LSM nor E(2) affected (P >or= 0.12) CYP2C or CYP3A mRNA expression or cellularity of the liver. Exogenous E(2) influenced circulating concentrations of IGF-I, T(3), and T(4). The estrogenic or anti-estrogenic potential of LSM is dependent upon the tissue, exposure to E(2), and the duration of LSM feeding. Feeding LSM during gestation, lactation, or during the grow-finish phase warrants further investigation.
authors: Department of Animal Sciences, North Dakota State University, Fargo, ND 58105, USA.
authors: M R
authors: MR
authors: O'Neil
authors: G P
authors: GP
authors: Lardy
authors: M E
authors: ME
authors: Wilson
authors: C O
authors: CO
authors: Lemley
authors: L P
authors: LP
authors: Reynolds
authors: J S
authors: JS
authors: Caton
authors: K A
authors: KA
authors: Vonnahme
mesh_terms: D000818:Animals; D002072:Butylene Glycols / Q000378:metabolism*; D051544:Cytochrome P-450 CYP3A / Q000235:genetics / Q000378:metabolism; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics / Q000378:metabolism; D004958:Estradiol / Q000378:metabolism / Q000494:pharmacology*; D005260:Female; D019597:Flax / Q000378:metabolism*; D018565:Food-Drug Interactions; D005753:Gastric Mucosa / Q000378:metabolism; D007334:Insulin-Like Growth Factor I / Q000032:analysis / Q000378:metabolism; D007421:Intestine, Small / Q000201:enzymology / Q000378:metabolism; D017705:Lignans / Q000378:metabolism*; D008099:Liver / Q000201:enzymology / Q000378:metabolism; D009929:Organ Size; D010052:Ovariectomy; D048789:Phytoestrogens / Q000378:metabolism*; D012313:RNA / Q000737:chemistry / Q000235:genetics; D011897:Random Allocation; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D012756:Sheep / Q000378:metabolism*; D013270:Stomach / Q000201:enzymology; D013974:Thyroxine / Q000032:analysis / Q000378:metabolism; D014284:Triiodothyronine / Q000032:analysis / Q000378:metabolism
chemical_list: D002072:Butylene Glycols; D017705:Lignans; D048789:Phytoestrogens; C400322:cytochrome P-450 CYP2C subfamily; D014284:Triiodothyronine; D004958:Estradiol; D012313:RNA; D007334:Insulin-Like Growth Factor I; D003577:Cytochrome P-450 Enzyme System; D051544:Cytochrome P-450 CYP3A; C060283:secoisolariciresinol; D013974:Thyroxine