title: Identification of a novel human gammapapillomavirus species.
abstract: By using random PCR amplification, shotgun sequencing and sequence similarity searches, we analysed nucleic acids present in cell cultures inoculated with samples from unexplained cases of encephalitis. We identified a divergent human papillomavirus (HPV) sequence originating from a rectal swab. The full genome was amplified by inverse PCR and sequenced. The prototype of the sixth gammapapillomavirus species, HPV116, was not found in the patient's cerebrospinal fluid or respiratory secretions, nor in culture supernatants from other unexplained cases of encephalitis, indicating that its identification in an encephalitis patient was accidental.
authors: Department of Laboratory Medicine, University of California, San Francisco, CA 94118, USA.|Blood Systems Research Institute, San Francisco, CA 94118, USA.
authors: Linlin
authors: L
authors: Li
authors: Department of Medicine, University of California, San Francisco, CA 94143, USA.
authors: Pennan
authors: P
authors: Barry
authors: Viral and Rickettsial Disease Laboratory, California Department of Public Health, Richmond, CA 94804, USA.
authors: Elaine
authors: E
authors: Yeh
authors: Viral and Rickettsial Disease Laboratory, California Department of Public Health, Richmond, CA 94804, USA.
authors: Carol
authors: C
authors: Glaser
authors: Viral and Rickettsial Disease Laboratory, California Department of Public Health, Richmond, CA 94804, USA.
authors: David
authors: D
authors: Schnurr
authors: Department of Laboratory Medicine, University of California, San Francisco, CA 94118, USA.|Blood Systems Research Institute, San Francisco, CA 94118, USA.
authors: Eric
authors: E
authors: Delwart
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D052689:Gammapapillomavirus / Q000145:classification* / Q000235:genetics*; D016679:Genome, Viral; D006801:Humans; D008253:Macaca mulatta; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D014777:Virus Diseases / Q000235:genetics