Development of predictive quantitative retention-activity relationship models of alkaloids by mixed micellar liquid chromatography.
The mixed micellar liquid chromatography is a mode that uses mixed micellar system of Brij35/SDS (85 : 15) as a mobile phase under adequate experimental conditions, can simulate the resting membrane potential and the conformation of the long hydrophilic polyoxyethylene chains remains unchanged. In this article, the applications of biopartitioning micellar chromatography, using mixed micellar system to describe and estimate bioactivities of alkaloids, has been focused. The BMC(Brij35/SDS)-QRAR models of half-life time, volume of distribution, plasma clearance and area under concentration-time curve were obtained using Brij35-SDS retention data. The aim is to take a look at the capability of the mixed micellar liquid chromatography model to describe and/or estimate the bioactivity of alkaloids.
Chen
Yu
Y
West China Hospital, Sichuan University, Chengdu, 610041, People's Republic of China.
Wu
Li-ping
LP
Chen
Cong
C
Ye
Li-ming
LM
D000465:Algorithms; D000470:Alkaloids / Q000737:chemistry* / Q000493:pharmacokinetics; D019540:Area Under Curve; D002851:Chromatography, High Pressure Liquid; D005544:Forecasting; D006207:Half-Life; D007202:Indicators and Reagents; D008823:Micelles; D011092:Polyethylene Glycols; D011237:Predictive Value of Tests; D012015:Reference Standards; D012967:Sodium Dodecyl Sulfate; D012984:Software; D012997:Solvents; D013056:Spectrophotometry, Ultraviolet; D013501:Surface-Active Agents
D000470:Alkaloids; D007202:Indicators and Reagents; D008823:Micelles; D012997:Solvents; D013501:Surface-Active Agents; D012967:Sodium Dodecyl Sulfate; D011092:Polyethylene Glycols; C515901:Brij 35