Developing compact multiphoton systems using femtosecond fiber lasers.
We implement a fiber-delivered compact femtosecond fiber laser at 1,030-nm wavelength in multiphoton imaging. The laser pulse duration is 150 fs, the average power is 200 mW, and the repetition rate is 40 MHz. The laser measures 200 x 160 x 45 mm in size and its output is delivered through a photonic bandgap fiber. Intrinsic second-harmonic generation signal is excited from rat tail tendon and human skin samples. Two-photon excited fluorescence signal is obtained from human skin tissues stained with exogenous fluorophore. Our results show that femtosecond fiber lasers at 1030-nm wavelength have significant potential in developing compact, all-fiber-based, portable multiphoton systems and endoscopes.
Tang
Shuo
S
Liu
Jian
J
Krasieva
Tatiana B
TB
Chen
Zhongping
Z
Tromberg
Bruce J
BJ
D000162:Acoustics; D000818:Animals; D005336:Fiber Optic Technology / Q000295:instrumentation / Q000379:methods*; D006801:Humans; D007834:Lasers; D036641:Microscopy, Fluorescence, Multiphoton / Q000295:instrumentation / Q000379:methods*; D051381:Rats; D012867:Skin / Q000033:anatomy & histology; D013623:Tail / Q000033:anatomy & histology; D013710:Tendons / Q000033:anatomy & histology