[Vasoactive intestinal peptide expression and its clinical significance in gastric adenocarcinoma].
OBJECTIVE
To investigate the expression of vasoactive intestinal peptide (VIP) in gastric adenocarcinoma, and to evaluate the correlation of VIP level with clinical pathologic parameters.


METHODS
The level of VIP in sera from gastric adenocarcinoma patients and healthy people was investigated by ELISA. Moreover, the differential gene expression between gastric adenocarcinoma, gastric dysplasia, and the corresponding normal gastric mucosa were determined by RT-PCR. Western Blot was also used to measure the expression of VIP in the gastric adenocarcinoma and the normal gastric mucosa.


RESULTS
The serum level of VIP was (5.794 +/- 0.014) ng/ ml in normal control and was (14.437 +/- 0.825) ng/ml in gastric adenocarcinoma patients, showing significant difference (P < 0.05). Meanwhile,the V/B of gastric adenocarcinoma tissues was greater than that of gastric dysplasia and the corresponding normal gastric mucosa (P <0.01), the values of V/B were 1.5261 +/- 0.3028, 0.9334 +/- 0.2872,and 0.9051 +/- 0.2794, respectively. The values of V/B between normal gastric mucosa and gastric dysplasia were not different significantly (P > 0.05). There were significantly negative correlation between the VIP mRNA expression of the differentiation degree of tumor (P < 0.05). The VIP mRNA expression was higher in gastric adenocarcinoma with lymph node metastasis than that without lymph node matastsis (P < 0.05). The VIP protein expression of the gastric adenocarcinoma tissues was greater than that of normal control.


CONCLUSIONS
This findings provide a direct evidence to support the possibility that VIP play a cofactor role in the pathogenesis of gastric adenocarcinoma.
Chen
Hui
H
Department of Digestion Medicine, The First Affiliated Hospital of Guangxi Medical University, Nanning 530021, China. chenhui680804@163.com
Cen
Ping
P
Li
Jia-quan
JQ
Lin
Yao-guang
YG
Jiang
Hai-xin
HX
Tang
Guo-du
GD
Zang
Ning
N
Feng
Zhen-bo
ZB
Su
Qi-jian
QJ
Xiao
Xin
X
D000230:Adenocarcinoma / Q000097:blood* / Q000235:genetics; D005753:Gastric Mucosa / Q000378:metabolism*; D015870:Gene Expression; D006801:Humans; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013274:Stomach Neoplasms / Q000097:blood* / Q000235:genetics; D014660:Vasoactive Intestinal Peptide / Q000097:blood* / Q000235:genetics
D012333:RNA, Messenger; D014660:Vasoactive Intestinal Peptide