Simultaneous detection of many T-cell specificities using combinatorial tetramer staining.
The direct detection of antigen-specific T cells using tetramers of soluble peptide-major histocompatibilty complex (pMHC) molecules is widely used in both basic and clinical immunology. However, the number of specificities that can be assessed simultaneously has been a major limitation. Here we describe and validate a method using combinations of fluorescent pMHC tetramers to simultaneously detect and enrich for many (>or=15) T-cell specificities in a single human blood sample.
Newell
Evan W
EW
Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, California, USA.
Klein
Lawrence O
LO
Yu
Wong
W
Davis
Mark M
MM
D018414:CD8-Positive T-Lymphocytes / Q000145:classification / Q000276:immunology; D002469:Cell Separation; D000939:Epitopes; D005434:Flow Cytometry; D005456:Fluorescent Dyes; D015234:HLA-A Antigens / Q000737:chemistry; D015789:HLA-A2 Antigen; D006801:Humans; D007158:Immunologic Techniques / Q000706:statistics & numerical data; D020836:Protein Structure, Quaternary; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000378:metabolism; D012680:Sensitivity and Specificity; D013194:Staining and Labeling / Q000379:methods; D016176:T-Lymphocyte Subsets / Q000145:classification / Q000276:immunology*
D000939:Epitopes; D005456:Fluorescent Dyes; D015234:HLA-A Antigens; C435939:HLA-A*02:01 antigen; D015789:HLA-A2 Antigen; D016693:Receptors, Antigen, T-Cell, alpha-beta