Myosin II tailpiece determines its paracrystal structure, filament assembly properties, and cellular localization.
Non muscle myosin II (NMII) is a major motor protein present in all cell types. The three known vertebrate NMII isoforms share high sequence homology but play different cellular roles. The main difference in sequence resides in the C-terminal non-helical tailpiece (tailpiece). In this study we demonstrate that the tailpiece is crucial for proper filament size, overcoming the intrinsic properties of the coiled-coil rod. Furthermore, we show that the tailpiece by itself determines the NMII filament structure in an isoform-specific manner, thus providing a possible mechanism by which each NMII isoform carries out its unique cellular functions. We further show that the tailpiece determines the cellular localization of NMII-A and NMII-B and is important for NMII-C role in focal adhesion complexes. We mapped NMII-C sites phosphorylated by protein kinase C and casein kinase II and showed that these phosphorylations affect its solubility properties and cellular localization. Thus phosphorylation fine-tunes the tailpiece effects on the coiled-coil rod, enabling dynamic regulation of NMII-C assembly. We thus show that the small tailpiece of NMII is a distinct domain playing a role in isoform-specific filament assembly and cellular functions.
Ronen
Daniel
D
Department of Biochemistry and Molecular Biology, the Institute for Medical Research, Israel-Canada, the Hebrew University, Hadassah Medical School, Jerusalem 91220, Israel.
Ravid
Shoshana
S
D000818:Animals; D047390:Casein Kinase II / Q000378:metabolism; D045744:Cell Line, Tumor; D018360:Crystallography, X-Ray / Q000379:methods; D005347:Fibroblasts / Q000378:metabolism; D006801:Humans; D051379:Mice; D008854:Microscopy, Electron / Q000379:methods; D024462:Myosin Type II / Q000378:metabolism / Q000502:physiology*; D010766:Phosphorylation; D020033:Protein Isoforms; D011493:Protein Kinase C / Q000378:metabolism; D017434:Protein Structure, Tertiary; D034741:RNA, Small Interfering / Q000378:metabolism; D014945:Wound Healing
D020033:Protein Isoforms; D034741:RNA, Small Interfering; D047390:Casein Kinase II; D011493:Protein Kinase C; D024462:Myosin Type II