title: [Establishment of a fluorescent quantitative PCR detection method for rabies virus and preparation of RNA positive controls].
abstract: OBJECTIVE
  Establish the fluorescent quantitative RT-PCR detection method for rabies virus (RV) and construct RNase-resistant virus-like particles as positive controls.
  
  
  METHODS
  Analyze the database in GenBank, the probe and the primers were designed in the conservative region of N gene of rabies virus and the method of real-time fluorescent quantitative PCR was obtained; On the basis of MS2 phage, with the technology of gene recombination, prepare the RNase-resistant virus-like particles for RV positive controls;
  
  
  RESULTS
  RNase-resistant virus-like particles were obtained after prokaryotic expression in E. coli. The designed primers and probe were confirmed to be very specific and conservative, and be sensitive to-concentration of 15 copies/microl.
  
  
  CONCLUSIONS
  Established the method of detecting rabies virus by reverse transcription real-time quantitative PCR,obtained the RNase-resistant and no infectivity virus-like particles as positive controls of rabies virus.
authors: College of Life Sciences, Henan Normal University, Xinxiang 453007, China. biowyl@126.com
authors: Yun-long
authors: YL
authors: Wang
authors: Rui-hong
authors: RH
authors: Zhao
authors: Zhi-tao
authors: ZT
authors: Li
authors: Yu-lin
authors: YL
authors: Li
authors: Guo-qiang
authors: GQ
authors: Wang
authors: Fei
authors: F
authors: Shen
mesh_terms: D000818:Animals; D015342:DNA Probes; D004279:DNA, Viral; D004285:Dogs; D006801:Humans; D012367:RNA, Viral / Q000032:analysis*; D011818:Rabies / Q000821:virology; D011820:Rabies virus / Q000737:chemistry*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D012259:Ribonuclease, Pancreatic / Q000378:metabolism; D019562:Viral Load
chemical_list: D015342:DNA Probes; D004279:DNA, Viral; D012367:RNA, Viral; D012259:Ribonuclease, Pancreatic