title: [Study on the biofilm early formation of Streptococcus mutans luxS gene mutation].
abstract: OBJECTIVE
  To investigate the effect of Streptococcus mutans luxS mutarotation on the early biofilm formation.
  
  
  METHODS
  Based on the immobilization of magnetic beads by adherent cells, an assay of biofilm quantitative analysis was developed for the kinetic quantification of biofilm formation in this study. Streptococcus mutans luxS mutant strain was constructed and subject to this biofilm luxS mutant strain were compared.
  
  
  RESULTS
  The delta luxS mutant started to form a biofilm from the 6th hour (delta BFI = 2.015), and the delta BFI of luxS mutant increased more quickly than that of the wild type strain, until reaching a complete immobilization of the beads after 10 hours (delta BFI = 7.025). The wild-type strain start to form a biofilm from the 10 th hour (delta BFI = 1.875) and the beads were completely immobilized between 12 and 14 hours.
  
  
  CONCLUSIONS
  The luxS mutation can accelerate biofilm on a polystyrene surface during the mid-exponential growth phase. And a luxS-dependent signal may play an important role in the early biofilm formation of Streptococcus mutans.
authors: Dept. of Cariology and Endodontology, Ninth People's Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine & Shanghai Research Insitute of Stomatology, Shanghai, China huangzhengwei@shsmu.edu.cn
authors: Zheng-wei
authors: ZW
authors: Huang
authors: Zheng
authors: Z
authors: Liu
authors: Zi-sheng
authors: ZS
authors: Tang
authors: Rui
authors: R
authors: Ma
authors: Cai-lian
authors: CL
authors: Zhu
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics*; D018441:Biofilms / Q000254:growth & development; D013437:Carbon-Sulfur Lyases / Q000235:genetics*; D017353:Gene Deletion; D015964:Gene Expression Regulation, Bacterial; D013295:Streptococcus mutans / Q000235:genetics* / Q000254:growth & development
chemical_list: D001426:Bacterial Proteins; D013437:Carbon-Sulfur Lyases; C117753:LuxS protein, Bacteria