[The role of PTEN-FAK signaling pathway in metastasis and invasive ability of leukemia cells].
OBJECTIVE
To investigate the effect of the wild type phosphatase and tensin homolog deleted on chromosome 10 (PTEN), a tumor-suppressor gene on the proliferation and apoptosis of human chronic myeloid leukemia (CML) cells line (K562) in vitro and explore the influence of PTEN-FAK signaling pathway on invasion and metastasis of leukemia cells.


METHODS
The recombinant Ad-PTEN gene containing green fluorescent protein gene (Ad-PTEN-GFP) or the empty vector (Ad-GFP) was transfected into K562 cells and fresh leukemia cells from CML patients in blast crisis. The growth of K562 cells was assayed by MTT assay; the apoptosis rate was assessed by flow cytometry (FCM). PTEN and FAK mRNA levels were detected by real-time fluorescent relative- quantification reverse transcriptional PCR (FQ-PCR) and its protein levels by Western blot. The metastasis and invasive ability was examined by transwell chamber assay.


RESULTS
The growth of K562 cells was suppressed markedly when Ad-PTEN-GFP was transfected into K562 cells at the 200 multiplicity of infection (MOI). The maximum growth inhibition rate was 35.2%. Transwell results showed the number of cells entered the lower chamber in Ad-GFP group was 9.1 fold more than that in Ad-PTEN-GFP group;The ability of metastasis and invasion of fresh leukemia cells was also suppressed after transfection with Ad-PTEN-GFP. FAK and p-FAK proteins were down-regulated by 0.72 and 0.16 fold lower after transfected with Ad-PTEN-GFP compared with Ad-GFP group.


CONCLUSIONS
PTEN gene might inhibit the proliferation, metastasis and invasive ability of leukemia cells via down-regulating FAK expression.
Cheng
Zhi-yong
ZY
Department of Hematology, the 2nd Hospital of Hebei Medical University, Shijiazhuang 050000, China.
Guo
Xiao-ling
XL
Li
Shi-hui
SH
Wang
Su-yun
SY
Yang
Xiao-yang
XY
Xue
Fang
F
Wen
Shu-peng
SP
Pan
Ling
L
D017209:Apoptosis; D002465:Cell Movement; D049109:Cell Proliferation; D051417:Focal Adhesion Kinase 1 / Q000235:genetics / Q000378:metabolism*; D005822:Genetic Vectors; D006801:Humans; D020014:K562 Cells; D017254:Leukemic Infiltration; D051059:PTEN Phosphohydrolase / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction; D014162:Transfection
D051417:Focal Adhesion Kinase 1; D051059:PTEN Phosphohydrolase; C494929:PTEN protein, human