Antifungal activity of CHE-23C, a dimeric sesquiterpene from Chloranthus henryi.
An antifungal compound was isolated from methanol extracts of stems and roots of Chloranthus henryi Hemsl. using ethyl acetate extraction and various chromatographic techniques. On the basis of spectroscopic analyses including mass and various NMR, the structure of the compound was identified as a dimeric sesquiterpene, CHE-23C. The compound showed potent antifungal activities (MICs = 1-32 microg/mL) in vitro against various phytopathogenic fungi such as Alternaria kikuchiana , Botrytis cinerea , Colletotrichum lagenarium , Magnaporthe grisea , Pythium ultimum , and Phytophthora infestans . In particular, it exhibited 91 and 100% disease-control activity in vivo against tomato late blight (P. infestans) and wheat leaf rust ( Puccinia recondita ) at concentrations of 33 and 100 microg/mL, respectively. The disease-control activity of this compound was stronger than that of the commercially available fungicide chlorothalonil, but weaker than that of dimethomorph. Therefore, the compound might serve as an interesting lead to develop effective antifungal agents.
Lee
Yun Mi
YM
Division of Biosystems Research, Korea Research Institute of Bioscience and Biotechnology, Daejeon 305-806, Korea.
Moon
Jae Sun
JS
Yun
Bong-Sik
BS
Park
Ki Duk
KD
Choi
Gyung Ja
GJ
Kim
Jin-Cheol
JC
Lee
Sang Han
SH
Kim
Sung Uk
SU
D001487:Basidiomycota / Q000187:drug effects; D005658:Fungi / Q000187:drug effects; D005659:Fungicides, Industrial / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D019684:Magnoliopsida / Q000737:chemistry*; D055750:Phytophthora infestans / Q000187:drug effects; D010935:Plant Diseases / Q000382:microbiology; D018517:Plant Roots / Q000737:chemistry; D018547:Plant Stems / Q000737:chemistry; D012717:Sesquiterpenes / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*
C543736:CHE-23C; D005659:Fungicides, Industrial; D012717:Sesquiterpenes