Compounds with neuroprotective activity from the medicinal plant Machilus thunbergii.
The dichloromethane fraction of the bark of Machilus thunbergii Sieb. et Zucc. (Lauraceae) significantly protected primary cultures of rat cortical cells exposed to the excitotoxic amino acid, L-glutamate. Through the activity-guided isolation from the CH(2)Cl(2) fraction, (+)-9'-hydroxygalbelgin (1), isogalcatin B (2), (7S,8S,8'R)-3',4'-dimethoxy-3,4,-methylenedioxylignan-7-ol (3), 1-hydroxy-7-hydroxymethyl-6-methoxyxanthone (4), 5,7-dimethoxy-3',4'-methylenedioxyflavan-3-ol (5), (+)-(3S,4S,6R)-3,6-dihydroxypiperitone (6), protocatechuic acid methyl ester (7) and tyrosol (8) were obtained. All of them had significant neuroprotective activities against glutamate-induced neurotoxicity in primary cultures of rat cortical cells at concentrations ranging from 0.1 microM to 10.0 microM and were comparable to MK-801, a well-known inhibitor of glutamate receptor.
Ma
Choong Je
CJ
School of Bioscience and Biotechnology, Kangwon National University, Chuncheon 200-701, Korea.
Kim
Young Choong
YC
Sung
Sang Hyun
SH
D000818:Animals; D002478:Cells, Cultured; D002525:Cerebellar Cortex / Q000166:cytology; D002942:Circular Dichroism; D005260:Female; D005663:Furans / Q000737:chemistry / Q000145:classification / Q000494:pharmacology; D018698:Glutamic Acid / Q000633:toxicity; D019770:Lauraceae / Q000737:chemistry*; D017705:Lignans / Q000737:chemistry / Q000145:classification / Q000494:pharmacology; D015394:Molecular Structure; D009474:Neurons / Q000187:drug effects*; D018696:Neuroprotective Agents / Q000737:chemistry* / Q000494:pharmacology*; D024301:Plant Bark / Q000737:chemistry*; D010946:Plants, Medicinal / Q000737:chemistry*; D051381:Rats; D017207:Rats, Sprague-Dawley
D005663:Furans; D017705:Lignans; D018696:Neuroprotective Agents; D018698:Glutamic Acid; C084077:galgravin