[Effect of demethylation treatment on the expression of inhibitory receptor KIR gene in NK-92MI cell line].
The aim of this study was to analyze the promoter methylation patterns of inhibitory killer cell immunoglobulin-like receptor (KIR) which gene expression and the effect of demethylation treatment were studied, and to explore the possible regulation mechanism of inhibitory kir gene expression. The promoter methylation levels of kir2DL1 and kir2DL2/kir2DL3 in NK-92MI cell line were detected by bisulfite sequencing technique. Then NK-92MI cells were treated with 5-azacytidine to induce the demethylation of CpG islands. The levels of gene expression of kir were determined by RT-PCR. The results demonstrated that the methylation frequencies of CpG dinucleotides surrounding the promoter regions of kir2DL1 and kir2DL2/kir2DL3 genes were 25% to 88% and 5% to 80% respectively. DNA-demethylating treatment with 5-azacytidine resulted in re-expression of kir2DL1 gene and increased expressions of kir2DL1, kir2DL2 and kir2DL3 genes in NK-92MI cells. In conclusion, the promoter DNA methylation participates in the regulation of kir gene expression in NK-92MI cells.
Gao
Xiao-Ning
XN
Department of Hematology, PLA General Hospital, Beijing, China.
Lin
Ji
J
Wang
Li-Li
LL
Gao
Li
L
Jin
Hai-Jie
HJ
Sun
Jing-Fen
JF
Yu
Li
L
D002460:Cell Line; D019175:DNA Methylation; D015870:Gene Expression; D006801:Humans; D007694:Killer Cells, Natural / Q000378:metabolism*; D054342:Receptors, KIR2DL1 / Q000235:genetics* / Q000378:metabolism; D054343:Receptors, KIR2DL2 / Q000235:genetics* / Q000378:metabolism; D054344:Receptors, KIR2DL3 / Q000235:genetics* / Q000378:metabolism
C093100:KIR2DL1 protein, human; C515077:KIR2DL2 protein, human; C515078:KIR2DL3 protein, human; D054342:Receptors, KIR2DL1; D054343:Receptors, KIR2DL2; D054344:Receptors, KIR2DL3