[The development and clinical application of papillomavirus genotyping by HPV DNA suspension array the development and clinical application of papillomavirus genotyping by HPV DNA suspension array].
OBJECTIVE
To develop a new platform for genotyping human papillomavirus(HPV) and to investigate its effect in clinical application.


METHODS
By combining L1 consensus PCR and multiplex hybridization using a Luminex xMAP system-based suspension array, we developed a rapid high-throughput assay,the HPV DNA suspension array (HPV-SA), capable of simultaneously typing 30 HPVs, including 18 high-risk HPV genotypes and 12 low-risk HPV genotypes. 810 clinical specimens were used to investigate the effect of HPV-SA. Veracity of the genotyping result was verified by E7 type-specific PCR-DNA sequencing.


RESULTS
Among the 810 clinical specimens, 243 were found to be HPV positive,including high-risk HPV subtypes 16, 18, 26, 31, 33, 39, 45, 51, 53, 56, 58, 59, 66, 68, 73 and 82,and low-risk HPV6, 11, 34, 54, 61, 67, 70 and 84. The sensitivity tested by standard samples was up to 10 copies of HPV DNA.


CONCLUSIONS
The HPV-SA developed here showed high sensitivity and specificity, suitable to be applied in clinical practice for HPV diagnosis and investigation on the prevalence of HPV sub-types.
Zhang
Su
S
Huzhou Maternity and Child Care Hospital, Huzhou 313000, China. zs@hzfby.com
Xing
Jian-ming
JM
Huang
Guo-ying
GY
Wu
Xiao-yun
XY
Wei
Xiao-fang
XF
Pan
Min
M
Min
Li-ping
LP
D000328:Adult; D000368:Aged; D019411:Clinical Laboratory Techniques; D005260:Female; D005838:Genotype; D006801:Humans; D008875:Middle Aged; D009693:Nucleic Acid Hybridization / Q000379:methods; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D027383:Papillomaviridae / Q000145:classification* / Q000235:genetics; D053918:Papillomavirus Vaccines / Q000145:classification / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods; D012680:Sensitivity and Specificity; D017422:Sequence Analysis, DNA; D013535:Suspensions; D002577:Uterine Cervical Diseases / Q000821:virology*; D055815:Young Adult
D053918:Papillomavirus Vaccines; D013535:Suspensions