title: In vitro and in vivo evaluation of akermanite bioceramics for bone regeneration.
abstract: This study investigated the effects of a calcium magnesium silicate bioceramic (akermanite) for bone regeneration in vitro and in vivo, with beta-tricalcium phosphate (beta-TCP) as a control. In vitro, the human bone marrow-derived mesenchymal stromal cells (hBMSCs) were cultured in an osteogenic medium supplemented with a certain concentration of two bioceramics' extracts for 20 days. An MTT assay showed that akermanite extract promoted proliferation of hBMSC significantly more than did beta-TCP extract. The results of alkaline phosphatase (ALP) activity test and the expression of osteogenic marker genes such as ALP, osteopontin (OPN), osteocalcin (OCN) and bone sialoprotein (BSP) demonstrated that the osteogenic differentiation of hBMSC was enhanced more by akermanite extract than by beta-TCP extract. In vivo, a histomorphology analysis and histomorphometry of the two porous bioceramics implants in rabbit femur defect models indicated that both in early- and late-stage implantations, akermanite promoted more osteogenesis and biodegradation than did beta-TCP; and in late-stage implantations, the rate of new bone formation was faster in akermanite than in beta-TCP. These results suggest that akermanite might be a potential and attractive bioceramic for tissue engineering.
authors: The Key Laboratory of Stem Cell Biology, Institute of Health Sciences, Chinese Academy of Sciences & Shanghai JiaoTong University School of Medicine, 225 South Chongqing Road, Shanghai 200025, People's Republic of China.
authors: Yan
authors: Y
authors: Huang
authors: Xiaogang
authors: X
authors: Jin
authors: Xiaoling
authors: X
authors: Zhang
authors: Hongli
authors: H
authors: Sun
authors: Jinwen
authors: J
authors: Tu
authors: Tingting
authors: T
authors: Tang
authors: Jiang
authors: J
authors: Chang
authors: Kerong
authors: K
authors: Dai
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry; D001854:Bone Marrow Cells / Q000166:cytology; D001861:Bone Regeneration; D002130:Calcium Phosphates / Q000737:chemistry; D049109:Cell Proliferation; D002478:Cells, Cultured; D002516:Ceramics / Q000737:chemistry*; D006801:Humans; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism; D016062:Porosity; D019736:Prostheses and Implants; D011817:Rabbits; D017154:Stromal Cells / Q000166:cytology / Q000378:metabolism
chemical_list: D001672:Biocompatible Materials; D002130:Calcium Phosphates; C485817:beta-tricalcium phosphate; C023506:Glass ceramics; D000469:Alkaline Phosphatase