A comparison of fluorescent DNA binding dyes for flow cytometric analysis of sporulating Saccharomyces cerevisiae.
Flow cytometry is important tool for investigating DNA replication in sporulating Saccharomyces cerevisiae. However, flow cytometry data from maturing spores is often difficult to interpret due to extensive broadening of the fluorescence peaks. This problem is markedly improved by treatment of the spores with potassium hydroxide prior to staining.
Raithatha
Sheetal A
SA
University of Alberta, Department of Biochemistry, 561 Medical Sciences Building, Edmonton, Alberta, Canada T6G 2H6.
Stuart
David T
DT
D004261:DNA Replication; D004271:DNA, Fungal / Q000378:metabolism*; D005434:Flow Cytometry / Q000379:methods*; D005456:Fluorescent Dyes / Q000494:pharmacology*; D012441:Saccharomyces cerevisiae / Q000502:physiology*; D013194:Staining and Labeling / Q000379:methods*
D004271:DNA, Fungal; D005456:Fluorescent Dyes