Introduction to proteomics.
In this chapter, the evolvement of proteomics from classical protein chemistry is depicted. The challenges of complexity and dynamics led to several new approaches and to the firm belief that a valuable proteomics technique has to be quantitative. Protein-based vs. peptide-based techniques, gel-based vs. non-gel-based proteomics, targeted vs. general proteomics, isotopic labeling vs. label-free techniques, and the importance of informatics are summarized and compared. A short outlook into the near future is given at the end of the chapter.
Lottspeich
Friedrich
F
Protein Analytics, Max-Planck-Institute of Biochemistry, Martinsried, Germany.
D019295:Computational Biology; D010446:Peptide Fragments / Q000032:analysis*; D011506:Proteins / Q000378:metabolism*; D040901:Proteomics / Q000379:methods*
D010446:Peptide Fragments; D011506:Proteins