AFLP genotyping of Candida metapsilosis clinical isolates: evidence for recombination.
In a collection of 395 independent clinical isolates classified as Candida parapsilosis on a biochemical profile basis, 20 Candida metapsilosis strains were identified by molecular tests with an isolation frequency of 5%. Isolates were screened for their susceptibility to conventionally used antifungals and for virulence determinants, such as biofilm formation and protease production. Molecular characterization of C. metapsilosis independent isolates by amplified fragment length polymorphism (AFLP) revealed a high percentage of polymorphic bands. Statistical analysis of the pairwise genetic distances and bootstrapping revealed that recombination occurs and significantly contributes to C. metapsilosis genetic population variability. No association between specific AFLP markers and drug resistance or other phenotypes was observed.
Hensgens
Lambert A M
LA
Dipartimento di Biologia, Unità di Genetica, Sezione Microbiologia, Italy.
Tavanti
Arianna
A
Mogavero
Selene
S
Ghelardi
Emilia
E
Senesi
Sonia
S
D054458:Amplified Fragment Length Polymorphism Analysis; D000935:Antifungal Agents / Q000494:pharmacology; D002175:Candida / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D002177:Candidiasis / Q000382:microbiology*; D016000:Cluster Analysis; D016172:DNA Fingerprinting / Q000379:methods*; D004271:DNA, Fungal / Q000235:genetics*; D019143:Evolution, Molecular; D005656:Fungal Proteins / Q000235:genetics; D005838:Genotype; D008826:Microbial Sensitivity Tests; D017720:Molecular Epidemiology; D016533:Mycological Typing Techniques; D011110:Polymorphism, Genetic; D011995:Recombination, Genetic; D037521:Virulence Factors / Q000235:genetics
D000935:Antifungal Agents; D004271:DNA, Fungal; D005656:Fungal Proteins; D037521:Virulence Factors