title: Tobacco xenobiotics release nitric oxide.
abstract: Many xenobiotic compounds exert their actions through the release of free radicals and related oxidants 12, bringing about unwanted biological effects 3. Indeed, oxidative events may play a significant role in tobacco toxicity from cigarette smoke. Here, we demonstrate the direct in vitro release of the free radical nitric oxide (*NO) from extracts and components of smokeless tobacco, including nicotine, nitrosonornicotine (NNN) and 4-(methyl-N-nitrosamino)-1-(3-pyridyl)-1-butanone (NNK) in phosphate buffered saline and human saliva using electron spin resonance and chemiluminescence detection. Our findings suggest that tobacco xenobiotics represent as yet unrecognized sources of *NO in the body.
authors: Department of Dentistry, Faculty of Medicine and Dentistry, University of Alberta, Edmonton, Canada. ernest.lam@ualberta.ca.
authors: Ewn
authors: E
authors: Lam
authors: Ee
authors: E
authors: Kelley
authors: Sm
authors: S
authors: Martin
authors: Gr
authors: G
authors: Buettner