Spleen cell proliferation during and after skin myiasis by human bot fly Dermatobia hominis.
Spleen cells from mice were examined at 5, 10, 15, 20 and 25 days post-infection (dpi) with Dermatobia hominis larva and at 5, 10, 15, 30 and 60 days post-larval emergence (dple). Cell proliferation in vitro assays were carried out with RPMI-1640 medium and larval secretory product (LSP) of D. hominis at 5, 10, 15, 20 and 25 days. When each group of mice was tested against each medium, significance was only seen for 25 dpi, with increasing order: LSP-10 d, -25 d, -5 d, -20 d, -15 d and RPMI. Significant results were also observed when each medium was tested against mice at each dpi or dple. Each dple group vs. each medium produced significant results only for 10 dple, with increasing order: LSP-5 d, -20 d, -25 d, -10 d, -15 d and RPMI. Comparative tests were also carried out between groups to refine certain observations. The LSPs were also analyzed using SDS-PAGE. The results prove that myiasis caused depletion of spleen cells, particularly under the effect of the LSP-10 and -15, but the cells tended to increase up to 60 dple. This in vitro assay may represent the real systemic immune response in the relationship LSP-D. hominis-host.
Gonçalves
Jomara Mendes
JM
Departamento de Parasitologia, Instituto de Ciências Biológicas, UFMG, Belo Horizonte, MG, Brazil.
Nascimento
Maria Fernanda Alves do
MF
Breyner
Natália Martins
NM
Fernandes
Viviane Cristina
VC
Góes
Alfredo Miranda de
AM
Leite
Antonio César Rios
AC
D000818:Animals; D049109:Cell Proliferation; D004175:Diptera; D007814:Larva; D008297:Male; D051379:Mice; D009198:Myiasis / Q000276:immunology / Q000473:pathology*; D012867:Skin / Q000469:parasitology* / Q000473:pathology; D013154:Spleen / Q000276:immunology / Q000473:pathology*; D013997:Time Factors