title: Dried-reswollen immobilized biocatalysts for detoxification of organophosphorous compounds in the flow systems.
abstract: New immobilized biocatalysts based on polypeptides containing N- or C-terminal polyhistidine sequences and possessing organophosphorus hydrolase activity were investigated for detoxification of organophosphorous neurotoxic compounds in the flow systems. The biocatalysts were revealed to have a high catalytic activity within wide pH and temperature ranges 7.5-12.5 degrees C and 15-65 degrees C, respectively. The immobilized biocatalysts can be dried and reswollen before use with 92-93% catalytic activity remaining after drying and rehydration procedures. The half-lives of the biocatalysts under wet and dry storage conditions were 420 and 540 days, respectively.
authors: Chemical Enzymology Department, Chemistry Faculty, The M.V. Lomonosov Moscow State University, Moscow, Russia. efremenko@enzyme.chem.msu.ru
authors: Elena N
authors: EN
authors: Efremenko
authors: Ilya V
authors: IV
authors: Lyagin
authors: Fatima M
authors: FM
authors: Plieva
authors: Igor Yu
authors: IY
authors: Galaev
authors: Bo
authors: B
authors: Mattiasson
mesh_terms: D000327:Adsorption; D043303:Aryldialkylphosphatase / Q000737:chemistry*; D002384:Catalysis; D003666:Decontamination / Q000379:methods*; D003890:Desiccation; D004800:Enzymes, Immobilized / Q000737:chemistry*; D009943:Organophosphorus Compounds / Q000737:chemistry* / Q000302:isolation & purification*; D012212:Rheology / Q000379:methods*
chemical_list: D004800:Enzymes, Immobilized; D009943:Organophosphorus Compounds; D043303:Aryldialkylphosphatase