title: Synergistic production of interleukin-23  by dendritic cells derived from cord  blood in response to costimulation  with LPS and IL-12.
abstract: This study was performed to provide insight for the optimization and regulation of immune homeostasis, which should be taken into account in the development of cell therapy using DCs and/or cytokine. Human CBDCs costimulated with LPS and IL-12 were examined for cytokine expression compared with ABDCs. Our results showed that costimulation with IL-12 and LPS in CBDCs resulted in increased expression of IL-23. Concomitantly, the phosphorylation of ERKs and p38 MAPK was increased, suggesting that these kinases are important signaling components for IL-23 induction in CBDC costimulated with LPS and IL-12. Furthermore, production of IL-23 in CBDC costimulated with LPS and IL-12 caused CD4(+)CD45RO(+) memory cells to increase IFN-gamma production. Taken together, CBDCs, costimulated with LPS and IL-12, show a synergistic increase in IL-23 production via enhanced phosphorylation of ERK1/2 and p38 MAPK and consequently, an induction of IFN-gamma production in the memory cells.
authors: Protein Engineering and Comparative Immunology, Department of Agricultural Biotechnology and Research Institute for Agriculture and Life Sciences, Seoul National University, Seoul 151-921, Republic of Korea.
authors: Mi Seon
authors: MS
authors: Jang
authors: Young Min
authors: YM
authors: Son
authors: Gi Rak
authors: GR
authors: Kim
authors: Yeo Jin
authors: YJ
authors: Lee
authors: Woon Kyu
authors: WK
authors: Lee
authors: Seok Ho
authors: SH
authors: Cha
authors: Seung Hyun
authors: SH
authors: Han
authors: Cheol-Heui
authors: CH
authors: Yun
mesh_terms: D003713:Dendritic Cells / Q000378:metabolism*; D004357:Drug Synergism; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism; D005312:Fetal Blood / Q000378:metabolism*; D006801:Humans; D007156:Immunologic Memory; D007371:Interferon-gamma / Q000096:biosynthesis; D018664:Interleukin-12 / Q000378:metabolism*; D053759:Interleukin-23 / Q000096:biosynthesis*; D008070:Lipopolysaccharides / Q000494:pharmacology*; D010766:Phosphorylation; D013601:T-Lymphocytes / Q000378:metabolism; D048051:p38 Mitogen-Activated Protein Kinases / Q000378:metabolism
chemical_list: D053759:Interleukin-23; D008070:Lipopolysaccharides; D018664:Interleukin-12; D007371:Interferon-gamma; D048049:Extracellular Signal-Regulated MAP Kinases; D048051:p38 Mitogen-Activated Protein Kinases