Generation of asparagine-linked glycan structure databases and their use.
Asparagine linked glycans (N-glycans) are important in biological processes. Yet, their structural complexity and lack of databases hinder progress in glycomics and glycobiology. We present a way for in silico generation of very large N-glycan structure databases and their use in high throughput composition and primary structure determination of N-glycans attached to peptides, based on CID (collision induced dissociation) MS/MS (tandem mass spectrometric data). The database and the integrated search engine is called Glyquest and is available to the glycomics community.
Gao
Hui Ying
HY
BioPharmaSoft, Boston, Massachusetts 02478, USA. h.gao@biopharmasoft.com
D001216:Asparagine / Q000737:chemistry*; D002240:Carbohydrate Sequence; D003628:Database Management Systems; D016208:Databases, Factual; D054794:Glycomics / Q000379:methods*; D016247:Information Storage and Retrieval / Q000379:methods*; D013058:Mass Spectrometry / Q000379:methods*; D011134:Polysaccharides / Q000737:chemistry*
D011134:Polysaccharides; D001216:Asparagine