title: Allele-specific regulation of MTTP expression influences the risk of ischemic heart disease.
abstract: Promoter polymorphisms in microsomal triglyceride transfer protein (MTTP) have been associated with decreased plasma lipids but an increased risk for ischemic heart disease (IHD), indicating that MTTP influences the susceptibility for IHD independent of plasma lipids. The objective of this study was to characterize the functional promoter polymorphism in MTTP predisposing to IHD and its underlying mechanism. Use of pyrosequencing technology revealed that presence of the minor alleles of the promoter polymorphisms -493G>T and -164T>C result in lower transcription of MTTP in vivo in the heart, liver, and macrophages. In vitro experiments indicated that the minor -164C allele mediates the lower gene expression and that C/EBP binds to the polymorphic region in an allele-specific manner. Furthermore, homozygous carriers of the -164C were found to have increased risk for IHD as shown in a case-control study including a total of 544 IHD patients and 544 healthy control subjects. We concluded that carriers of the minor -164C allele have lower expression of MTTP in the heart, mediated at least partly by the transcription factor CCAAT/enhancer binding protein, and that reduced concentration of MTTP in the myocardium may contribute to IHD upon ischemic damage.
authors: Atherosclerosis Research Unit, Department of Medicine, Center for Molecular Medicine, Karolinska Institutet, Stockholm, Sweden.
authors: Anna
authors: A
authors: Aminoff
authors: Helena
authors: H
authors: Ledmyr
authors: Petra
authors: P
authors: Thulin
authors: Kerstin
authors: K
authors: Lundell
authors: Leyla
authors: L
authors: Nunez
authors: Elisabeth
authors: E
authors: Strandhagen
authors: Charlotte
authors: C
authors: Murphy
authors: Ulf
authors: U
authors: Lidberg
authors: Jukka
authors: J
authors: Westerbacka
authors: Anders
authors: A
authors: Franco-Cereceda
authors: Jan
authors: J
authors: Liska
authors: Lars Bo
authors: LB
authors: Nielsen
authors: Mats
authors: M
authors: Gåfvels
authors: Maria Nastase
authors: MN
authors: Mannila
authors: Anders
authors: A
authors: Hamsten
authors: Hannele
authors: H
authors: Yki-Järvinen
authors: Dag
authors: D
authors: Thelle
authors: Per
authors: P
authors: Eriksson
authors: Jan
authors: J
authors: Borén
authors: Ewa
authors: E
authors: Ehrenborg
mesh_terms: D000368:Aged; D000483:Alleles; D001483:Base Sequence; D022762:CCAAT-Enhancer-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D002352:Carrier Proteins / Q000235:genetics*; D016022:Case-Control Studies; D005234:Fatty Liver / Q000235:genetics / Q000378:metabolism*; D005260:Female; D005786:Gene Expression Regulation; D006367:HeLa Cells; D006321:Heart / Q000502:physiology; D006801:Humans; D008099:Liver / Q000378:metabolism; D008264:Macrophages / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D009000:Monocytes / Q000378:metabolism; D017202:Myocardial Ischemia / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide; D011401:Promoter Regions, Genetic; D020218:Response Elements / Q000235:genetics
chemical_list: D022762:CCAAT-Enhancer-Binding Proteins; D002352:Carrier Proteins; C106092:microsomal triglyceride transfer protein