Isolation of plasma membranes from the nervous system by countercurrent distribution in aqueous polymer two-phase systems.
The plasma membrane separates the cell-interior from the cell's environment. To maintain homeostatic conditions and to enable transfer of information, the plasma membrane is equipped with a variety of different proteins such as transporters, channels, and receptors. The kind and number of plasma membrane proteins are a characteristic of each cell type. Owing to their location, plasma membrane proteins also represent a plethora of drug targets. Their importance has entailed many studies aiming at their proteomic identification and characterization. Therefore, protocols are required that enable their purification in high purity and quantity. Here, we report a protocol, based on aqueous polymer two-phase systems, which fulfils these demands. Furthermore, the protocol is time-saving and protects protein structure and function.
Schindler
Jens
J
Abteilung Neurogenetik, Institut für Biologie und Umweltwissenschaften, Carl von Ossietzky Universität, 21111 Oldenburg, Germany.
Nothwang
Hans Gerd
HG
D001923:Brain Chemistry; D002462:Cell Membrane / Q000737:chemistry*; D006801:Humans; D008565:Membrane Proteins / Q000032:analysis*; D044367:Phase Transition; D011108:Polymers / Q000737:chemistry; D040901:Proteomics / Q000379:methods*; D014867:Water / Q000737:chemistry
D008565:Membrane Proteins; D011108:Polymers; D014867:Water