title: Simultaneous determination of eight major steroids from Polyporus umbellatus by high-performance liquid chromatography coupled with mass spectrometry detections.
abstract: Polyporus umbellatus is a widely used diuretic herbal medicine. In this study, a high-performance liquid chromatography coupled with atmospheric pressure chemical ionization-mass spectrometric detection (HPLC-APCI-MS) method was developed for qualitative and quantitative analysis of steroids, as well as for the quality control of Polyporus umbellatus. The selectivity, reproducibility and sensitivity were compared with HPLC with photodiode array detection and evaporative light scattering detection (ELSD). Selective ion monitoring in positive mode was used for qualitative and quantitative analysis of eight major components and beta-ecdysterone was used as the internal standard. Limits of detection and quantification fell in the ranges 7-21 and 18-63 ng/mL for the eight analytes with an injection of 10 microL samples, and all calibration curves showed good linear regression (r(2) > 0.9919) within the test range. The quantitative results demonstrated that samples from different localities showed different qualities. Advantages, in comparison with conventional HPLC-diode array detection and HPLC-ELSD, are that reliable identification of target compounds could be achieved by accurate mass measurements along with characteristic retention time, and the great enhancement in selectivity and sensitivity allows identification and quantification of low levels of constituents in complex Polyporus umbellatus matrixes.
authors: Biomedicine Key Laboratory of Shaanxi Province, Northwest University, 229 Taibai North Road, Xi'an, Shaanxi, 710069, China.
authors: Ying-yong
authors: YY
authors: Zhao
authors: Xian-long
authors: XL
authors: Cheng
authors: Yongmin
authors: Y
authors: Zhang
authors: Ye
authors: Y
authors: Zhao
authors: Rui-chao
authors: RC
authors: Lin
authors: Wen-ji
authors: WJ
authors: Sun
mesh_terms: D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D007202:Indicators and Reagents; D013058:Mass Spectrometry; D011124:Polyporaceae / Q000737:chemistry*; D012015:Reference Standards; D015203:Reproducibility of Results; D012996:Solutions; D013256:Steroids / Q000032:analysis*; D014465:Ultrasonics
chemical_list: D007202:Indicators and Reagents; D012996:Solutions; D013256:Steroids