Activation tagging of ADR2 conveys a spreading lesion phenotype and resistance to biotrophic pathogens.
An Arabidopsis PR1::luciferase (LUC) transgenic line was transformed with activation T-DNA tags and the resulting population screened for dominant gain-of-function mutants exhibiting constitutive LUC activity. LUC imaging identified activated disease resistance 2 (adr2), which exhibited slowly spreading lesions in the absence of pathogen challenge. Molecular, genetic and histochemical analysis was employed to characterize this mutant in detail. adr2 plants constitutively expressed defence-related and antioxidant genes. Moreover, this line accrued increased quantities of salicylic acid (SA) and exhibited heightened mitogen-activated protein kinase activity. adr2 plants exhibited increased resistance against numerous biotrophic but not necrotrophic pathogens. The adr2 phenotype resulted from the overexpression of a Toll interleukin receptor (TIR) nucleotide binding site (NBS) leucine rich repeat (LRR) gene (At1g56510). Constitutive PR1 expression was completely abolished in adr2 nahG, adr2 npr1 and adr2 eds1 double mutants. Furthermore, heightened resistance against Hyaloperonospora arabidopsis Noco2 was compromised in adr2 nahG and adr2 eds1 double mutants but not in adr2 npr1, adr2 coi1 or adr2 etr1 plants. These data imply that adr2-mediated resistance operates through an Enhanced Disease Susceptibility (EDS) and SA-dependent defence signalling network which functions independently from COI1 or ETR1.
Aboul-Soud
Mourad A M
MAM
College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Kingdom of Saudi Arabia.
Chen
Xinwei
X
Institute of Molecular Plant Sciences, School of Biological Sciences, University of Edinburgh, King's Buildings, Edinburgh EH9 3JR, UK.
Kang
Jeong-Gu
JG
Institute of Molecular Plant Sciences, School of Biological Sciences, University of Edinburgh, King's Buildings, Edinburgh EH9 3JR, UK.
Yun
Byung-Wook
BW
Institute of Molecular Plant Sciences, School of Biological Sciences, University of Edinburgh, King's Buildings, Edinburgh EH9 3JR, UK.
Raja
M Usman
MU
Institute of Molecular Plant Sciences, School of Biological Sciences, University of Edinburgh, King's Buildings, Edinburgh EH9 3JR, UK.
Malik
Saad I
SI
Institute of Molecular Plant Sciences, School of Biological Sciences, University of Edinburgh, King's Buildings, Edinburgh EH9 3JR, UK.
Loake
Gary J
GJ
Institute of Molecular Plant Sciences, School of Biological Sciences, University of Edinburgh, King's Buildings, Edinburgh EH9 3JR, UK.
D017360:Arabidopsis / Q000235:genetics / Q000382:microbiology*; D029681:Arabidopsis Proteins / Q000235:genetics*; D002734:Chlorophyll / Q000378:metabolism; D002874:Chromosome Mapping; D004269:DNA, Bacterial; D005658:Fungi / Q000472:pathogenicity*; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D007113:Immunity, Innate / Q000235:genetics; D008156:Luciferases / Q000235:genetics; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism; D009154:Mutation; D009868:Oomycetes / Q000472:pathogenicity*; D010935:Plant Diseases / Q000382:microbiology*; D010940:Plant Proteins / Q000235:genetics*; D030821:Plants, Genetically Modified; D020156:Salicylic Acid / Q000378:metabolism; D015398:Signal Transduction
D029681:Arabidopsis Proteins; D004269:DNA, Bacterial; D010940:Plant Proteins; C036483:T-DNA; C532021:WRR4 protein, Arabidopsis; C053376:pathogenesis-related proteins, plant; D002734:Chlorophyll; D008156:Luciferases; D020928:Mitogen-Activated Protein Kinases; D020156:Salicylic Acid