Recovery of E,E-farnesol from cultures of yeast erg9 mutants: extraction with polymeric beads and purification by normal-phase chromatography.
Saccharomyces cerevisiae erg9 mutants blocked at squalene synthase require ergosterol for growth and produce E,E-farnesol. Typically, at least half the amount of farnesol remains cell associated. Practically insoluble in water, farnesol can be extracted from production cultures of the erg9 mutants using either methanol/hexane or poly(styrene-co-divinylbenzene) beads. The first method consumes more solvents and requires centrifugation to clear an interface emulsion. The second method uses 50% less solvent and the beads can be used repeatedly for extraction. The solvent-free crude extract from the beads extraction contained higher concentration of farnesol (76-77%) than that from the solvent extraction (61-65%). Farnesol was obtained after normal-phase chromatography in high overall recovery (94%) and purity (99%).
Song
Linsheng
L
Bio-Technical Resources, 1035 South 7th Street, Manitowoc, WI 54220, USA. song@biotechresources.com
D002845:Chromatography / Q000295:instrumentation / Q000379:methods*; D005204:Farnesol / Q000302:isolation & purification* / Q000378:metabolism; D013186:Farnesyl-Diphosphate Farnesyltransferase / Q000235:genetics* / Q000378:metabolism; D009154:Mutation; D012117:Resins, Synthetic / Q000737:chemistry; D012441:Saccharomyces cerevisiae / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics* / Q000378:metabolism
D012117:Resins, Synthetic; D029701:Saccharomyces cerevisiae Proteins; D005204:Farnesol; C496127:ERG9 protein, S cerevisiae; D013186:Farnesyl-Diphosphate Farnesyltransferase