title: Display and selection of scFv antibodies on HEK-293T cells.
abstract: We describe a human cell display strategy to isolate high-affinity single-chain antibody fragments (scFvs) specific for CD22 for the treatment of B-cell malignancies. Our strategy uses flow cytometry and human embryonic kidney 293T (HEK-293T) cells that are widely used for transient protein expression. Flow cytometry enhances the screen's sensitivity thereby allowing us to isolate high-affinity scFvs. Using human cell display, one could isolate and engineer scFvs, single domains, Fabs, or whole IgGs for increased affinity and other biological functions.
authors: Laboratory of Molecular Biology, National Cancer Institute, Bethesda, MD 20892-4264, USA.
authors: Mitchell
authors: M
authors: Ho
authors: Ira
authors: I
authors: Pastan
mesh_terms: D000911:Antibodies, Monoclonal / Q000276:immunology*; D002478:Cells, Cultured; D005434:Flow Cytometry / Q000379:methods*; D006801:Humans; D007135:Immunoglobulin Variable Region / Q000276:immunology* / Q000302:isolation & purification*; D007668:Kidney / Q000196:embryology; D019151:Peptide Library; D051918:Sialic Acid Binding Ig-like Lectin 2 / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; C497867:CD22 protein, human; D007135:Immunoglobulin Variable Region; D019151:Peptide Library; D051918:Sialic Acid Binding Ig-like Lectin 2