Static magnetic field attenuates mortality rate of mice by increasing the production of IL-1 receptor antagonist.
OBJECTIVE
Disseminated intravascular coagulation (DIC) is a complex systemic thrombohemorrhagic disorder involving intravascular coagulation and hemorrhage. The aim of this study is to test whether static magnetic field (SMF) is effective in attenuating lipopolysaccharide (LPS)-induced DIC.


METHODS
In vivo experiments were performed in this study using male BALB/cByJ mice. An intraperitoneal injection of 50 mg/kg LPS was shown to lead to approximately 50% mortality and this dose was used in subsequent experiments. To test the effects of SMF on the survival rate of LPS-induced animals, the mice were exposed to 0.25-T SMF for 2 h before LPS injection. In addition, the effect of a 2-h SMF treatment on the production of anti-inflammatory cytokines was evaluated.


RESULTS
In the first set of experiments, we found that the survival rate was higher in the SMF-exposed group than in the sham-exposed group. The circulating platelet (PLT) counts in the SMF-exposed mice were significantly higher than in the unexposed animals. However, no significant changes in inflammatory cytokine, including tumour necrosis factor-alpha (TNF-alpha), interleukin-1alpha (IL-1alpha), interleukin-6 (IL-6) and monocyte chemotactic protein 1 (MCP-1), in plasma were found after SMF treatment. The results from the second experiment showed that the plasma levels of interleukin-1 receptor antagonist (IL-1ra) were higher in the SMF-exposed group than in the sham group.


CONCLUSIONS
Exposure to an SMF increases the plasma levels of IL-1ra. This effect may inhibit the reduction in PLT in plasma, resulting in prevention in LPS induced DIC.
Lin
Shu-Li
SL
Dental Department, Cathay General Hospital, Taipei, Taiwan.
Chang
Wei-Jen
WJ
Lin
Yung-Sheng
YS
Ou
Keng-Liang
KL
Lin
Che-Tong
CT
Lin
Chih-Ping
CP
Huang
Haw-Ming
HM
D000818:Animals; D004211:Disseminated Intravascular Coagulation / Q000401:mortality*; D053590:Interleukin 1 Receptor Antagonist Protein / Q000096:biosynthesis*; D008070:Lipopolysaccharides / Q000633:toxicity; D008280:Magnetics; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D010976:Platelet Count
D053590:Interleukin 1 Receptor Antagonist Protein; D008070:Lipopolysaccharides