title: Differences in expression and activity of 11beta-hydroxysteroid dehydrogenase type 1 and 2 in human placentas of term pregnancies according to birth weight and gender.
abstract: BACKGROUND
  Fetal exposure to maternal glucocorticoids may determine fetal growth and the programing of later disorders. Availability of the glucocorticoids in the placenta is regulated by the 11beta-hydroxysteroid dehydrogenase (11beta-HSDs) enzymes. To date, there are discrepancies with regard to cortisol (F) cord blood levels in fetuses with intrauterine growth retardation in different species. Objective To study the expression and activity of 11beta-HSDs in placentas from full term small for gestational age (SGA), appropriate for gestational age (AGA) and large for gestational age (LGA) newborns, and cortisol cord blood concentration.
  
  
  METHODS
  Twenty-five placentas from AGA, 24 SGA and 25 LGA were collected.
  
  
  RESULTS
  SGA newborns had significantly lower and LGA newborns had significantly higher birth weight, birth length, head circumference, and placental weight than AGA counterparts. We observed a direct correlation between placental weight and birth weight, birth length and head circumference, and higher cord F levels in SGA newborns. The 11beta-HSD1 expression was similar among the SGA, AGA, and LGA placentas. However, within the placentas of SGA newborns, the 11beta-HSD1 mRNA levels were significantly reduced in the chorionic plate compared with basal plate. An inverse correlation between cord F levels and activity of 11beta-HSD1 in the chorionic plate of the SGA placentas was detected. The 11beta-HSD2 activity was seven- to eightfold higher compared with 11beta-HSD1 in the placentas, and there was a lower 11beta-HSD2 activity in females' SGA placentas compared with the male SGA placentas.
  
  
  CONCLUSIONS
  We observed a lower expression and activity of 11beta-HSD1 in the chorionic plate of the SGA placentas, suggesting a possible compensatory mechanism to diminish the higher cortisol fetal concentrations observed in fetuses with intrauterine growth restriction.
authors: Institute of Maternal and Child Research, Faculty of Medicine, University of Chile, Santa Rosa 1234, 2 degrees piso, Casilla 226-3, Santiago, Chile.
authors: V
authors: V
authors: Mericq
authors: P
authors: P
authors: Medina
authors: E
authors: E
authors: Kakarieka
authors: L
authors: L
authors: Márquez
authors: M C
authors: MC
authors: Johnson
authors: G
authors: G
authors: Iñiguez
mesh_terms: D043205:11-beta-Hydroxysteroid Dehydrogenase Type 1 / Q000235:genetics* / Q000378:metabolism; D043209:11-beta-Hydroxysteroid Dehydrogenase Type 2 / Q000235:genetics* / Q000378:metabolism; D001724:Birth Weight / Q000235:genetics*; D002823:Chorion / Q000201:enzymology / Q000378:metabolism; D005260:Female; D005317:Fetal Growth Retardation / Q000201:enzymology / Q000235:genetics / Q000378:metabolism; D015971:Gene Expression Regulation, Enzymologic; D005865:Gestational Age; D006801:Humans; D007231:Infant, Newborn; D007236:Infant, Small for Gestational Age / Q000378:metabolism; D008297:Male; D010920:Placenta / Q000378:metabolism*; D011247:Pregnancy; D011263:Pregnancy Trimester, Third / Q000235:genetics* / Q000378:metabolism; D012737:Sex Factors; D047929:Term Birth / Q000235:genetics* / Q000378:metabolism
chemical_list: D043205:11-beta-Hydroxysteroid Dehydrogenase Type 1; D043209:11-beta-Hydroxysteroid Dehydrogenase Type 2