Association between sexual precocity and alleles of KISS-1 and GPR54 genes in goats.
KISS-1 and GPR54 were regarded as key regulators for the puberty onset and fundamental gatekeepers of sexual maturation in mammals. To explore the possible association between variations in KISS-1 and GPR54 with sexual precocity, mutation screening of exon 1 of KISS-1 and exon 1, exon 3, and partial exon 5 of GPR54 was performed in a sexual precocious breed (Jining Grey goats) and sexual late-maturing breeds (Inner Mongolia Cashmere, Angora, and Boer goats) by PCR-SSCP. The results showed that five novel mutations were identified in exon 1 and partial exon 5 of GPR54 including C96 T, T173C, G176A, G825A, and C981 T. The Jining Grey goats with genotype BB or AB had 1.07 (P < 0.05) or 0.40 (P < 0.05) kids more than those with AA. The Jining Grey goats with genotype DD or CD had 1.80 (P < 0.05) or 0.55 (P < 0.05) kids more than CC, respectively. The present study preliminarily showed an association between alleles B and D of GPR54 with high litter size and sexual precocity in Jining Grey goats.
Feng
T
T
Key Laboratory of Farm Animal Genetic Resources and Utilization of Ministry of Agriculture, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, P.R. China.
Zhao
Y Z
YZ
Chu
M X
MX
Zhang
Y J
YJ
Fang
L
L
Di
R
R
Cao
G L
GL
Li
N
N
D000367:Age Factors; D000483:Alleles; D000818:Animals; D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics; D005091:Exons; D005260:Female; D005838:Genotype; D006041:Goats / Q000235:genetics* / Q000502:physiology*; D008098:Litter Size / Q000235:genetics; D011247:Pregnancy; D011506:Proteins / Q000235:genetics; D043562:Receptors, G-Protein-Coupled / Q000235:genetics*; D012741:Sexual Maturation / Q000235:genetics*; D013045:Species Specificity
D017931:DNA Primers; D011506:Proteins; D043562:Receptors, G-Protein-Coupled