The effect of moesin overexpression on ageing of human dermal microvascular endothelial cells.
Senescence of microvascular endothelial cells is known to play an important role in the pathophysiology of vascular diseases related to ageing, but the accurate mechanism or related genes are not known. Moesin, a cytoskeletal protein and the most potent candidate as an ageing-related protein, showed obvious changes in expression when compared before and after ageing. In this study, a lentivirus was used to overexpress moesin in endothelial cells. The expression of cell cycle mediators such as p16, cyclin D1 and cdk4, which can be the markers of ageing, was compared by RNA and was shown to be suppressed in moesin overexpressed endothelial cells. In conclusion, it can be said that the expression of moesin delays senescence of human dermal microvascular endothelial cells and this fundamental discovery can be used as a basis for understanding the mechanism of ageing and age-related diseases.
Lee
Ju Hee
JH
Hong
In Ae
IA
Oh
Sang Ho
SH
Kwon
Yeon Sook
YS
Cho
Soo Hyun
SH
Lee
Kwang Hoon
KH
D000375:Aging; D002453:Cell Cycle; D002478:Cells, Cultured; D016922:Cellular Senescence; D003599:Cytoskeleton / Q000378:metabolism; D004730:Endothelium, Vascular / Q000166:cytology / Q000378:metabolism*; D005786:Gene Expression Regulation; D006801:Humans; D008833:Microcirculation; D008840:Microfilament Proteins / Q000096:biosynthesis* / Q000378:metabolism; D008856:Microscopy, Fluorescence / Q000379:methods; D008858:Microscopy, Phase-Contrast / Q000379:methods; D008954:Models, Biological; D012333:RNA, Messenger / Q000378:metabolism; D012867:Skin / Q000378:metabolism*
D008840:Microfilament Proteins; D012333:RNA, Messenger; C071058:moesin