Social stress enhances IL-1beta and TNF-alpha production by Porphyromonas gingivalis lipopolysaccharide-stimulated CD11b+ cells.
Psychological stress is associated with an increased expression of markers of peripheral inflammation, and there is a growing literature describing a link between periodontal pathogens and systemic inflammation. The hypothesis of the present work is that exposing mice to the social stressor, called social disruption (SDR), would enhance the inflammatory response to lipopolysaccharide (LPS) derived from the oral pathogen, Porphyromonas gingivalis. Mice were exposed to SDR for 2h per day on 6 consecutive days. On the morning following the last cycle of SDR, mice were tested for anxiety-like behavior in the open field test and novel object test. The mice were sacrificed the following day and their spleens harvested. Spleen cells were stimulated with LPS derived from P. gingivalis in the absence or presence of increasing doses of corticosterone. Social disruption resulted in anxiety-like behavior, and the production of IL-1beta and TNF-alpha was significantly higher in spleen cells from mice exposed to SDR in comparison to levels from non-stressed control mice. In addition, the viability of spleen cells from mice exposed to SDR was significantly greater than the viability of cells from non-stressed control mice, even in the presence of high doses of corticosterone. The use of cultures enriched for CD11b+ cells indicated that the stressor was affecting the activity of splenic myeloid cells. This study demonstrates that social stress enhances the inflammatory response to an oral pathogen and could provide a critical clue in the reported associations between stress, inflammation, and oral pathogens.
Bailey
Michael T
MT
Section of Oral Biology, College of Dentistry, The Ohio State University, Columbus, OH 43210, United States. Bailey.494@osu.edu
Kinsey
Steven G
SG
Padgett
David A
DA
Sheridan
John F
JF
Leblebicioglu
Binnaz
B
D000818:Animals; D000850:Anomie; D039481:CD11b Antigen / Q000378:metabolism*; D002470:Cell Survival; D002478:Cells, Cultured; D005106:Exploratory Behavior / Q000502:physiology; D053583:Interleukin-1beta / Q000096:biosynthesis*; D008070:Lipopolysaccharides; D008264:Macrophages / Q000378:metabolism; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D016966:Porphyromonas gingivalis / Q000378:metabolism; D013154:Spleen / Q000276:immunology* / Q000378:metabolism*; D013315:Stress, Psychological / Q000276:immunology*; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis*
D039481:CD11b Antigen; D053583:Interleukin-1beta; D008070:Lipopolysaccharides; D014409:Tumor Necrosis Factor-alpha