MCC, a new interacting protein for Scrib, is required for cell migration in epithelial cells.
To further characterize the molecular events supporting the tumor suppressor activity of Scrib in mammals, we aim to identify new binding partners. We isolated MCC, a recently identified binding partner for beta-catenin, as a new interacting protein for Scrib. MCC interacts with both Scrib and the NHERF1/NHERF2/Ezrin complex in a PDZ-dependent manner. In T47D cells, MCC and Scrib proteins colocalize at the cell membrane and reduced expression of MCC results in impaired cell migration. By contrast to Scrib, MCC inhibits cell directed migration independently of Rac1, Cdc42 and PAK activation. Altogether, these results identify MCC as a potential scaffold protein regulating cell movement and able to bind Scrib, beta-catenin and NHERF1/2.
Arnaud
Camille
C
INSERM UMR891, Centre de Recherche en Cancérologie de Marseille, Pharmacologie Moléculaire, Marseille F-13009, France.
Sebbagh
Michaël
M
Nola
Sébastien
S
Audebert
Stéphane
S
Bidaut
Ghislain
G
Hermant
Aurélie
A
Gayet
Odile
O
Dusetti
Nelson J
NJ
Ollendorff
Vincent
V
Santoni
Marie-Josée
MJ
Borg
Jean-Paul
JP
Lécine
Patrick
P
D000818:Animals; D002460:Cell Line; D002465:Cell Movement / Q000502:physiology*; D004789:Enzyme Activation; D004847:Epithelial Cells / Q000166:cytology / Q000502:physiology*; D006801:Humans; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D010750:Phosphoproteins / Q000235:genetics / Q000378:metabolism; D011485:Protein Binding; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D017923:Sodium-Hydrogen Exchangers / Q000235:genetics / Q000378:metabolism; D025521:Tumor Suppressor Proteins / Q000235:genetics / Q000378:metabolism*; D051176:beta Catenin / Q000378:metabolism; D020764:cdc42 GTP-Binding Protein / Q000378:metabolism; D020830:rac1 GTP-Binding Protein / Q000378:metabolism
D008565:Membrane Proteins; D010750:Phosphoproteins; D011993:Recombinant Fusion Proteins; C480591:SCRIB protein, human; D017923:Sodium-Hydrogen Exchangers; D025521:Tumor Suppressor Proteins; D051176:beta Catenin; C093376:sodium-hydrogen exchanger regulatory factor; C067857:MCC protein, human; D020764:cdc42 GTP-Binding Protein; D020830:rac1 GTP-Binding Protein