title: A Bombyx mandarina mutant exhibiting translucent larval skin is controlled by the molybdenum cofactor sulfurase gene.
abstract: During the maintenance of the wild silkworm, Bombyx mandarina, a mutant phenotype exhibiting translucent skin was identified. Based on the crossing experiments with the domesticated silkworm, Bombyx mori, we found that the mutant was controlled by molybdenum cofactor sulfurase (MoCoS) gene. We designated the mutant ''Ozaki's translucent'' (og(Z)). We found a 2.1-kb deletion containing the transcription initiation site, exons 1 and 2, and the 5' end of exon 3 of the MoCoS gene. The transcript of the MoCoS gene was not detected in the og(Z) homozygote. We concluded that og(Z) is a complete loss-of-function allele generated by a disruption of the MoCoS gene.
authors: Department of Agricultural and Environmental Biology, Graduate School of Agricultual and Life Sciences, The University of Tokyo, Bunkyo-ku, Tokyo, Japan.
authors: Tsuguru
authors: T
authors: Fujii
authors: Masataka
authors: M
authors: Ozaki
authors: Takaaki
authors: T
authors: Masamoto
authors: Susumu
authors: S
authors: Katsuma
authors: Hiroaki
authors: H
authors: Abe
authors: Toru
authors: T
authors: Shimada
mesh_terms: D000818:Animals; D012831:Bombyx / Q000201:enzymology / Q000235:genetics* / Q000378:metabolism; D003001:Cloning, Molecular; D003067:Coenzymes / Q000378:metabolism; D003433:Crosses, Genetic; D005260:Female; D015971:Gene Expression Regulation, Enzymologic; D019476:Insect Proteins / Q000235:genetics* / Q000378:metabolism; D007814:Larva / Q000201:enzymology / Q000235:genetics / Q000378:metabolism; D008297:Male; D008667:Metalloproteins / Q000378:metabolism; D008969:Molecular Sequence Data; D000090006:Molybdenum Cofactors; D009154:Mutation; D011621:Pteridines / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D013466:Sulfurtransferases / Q000235:genetics* / Q000378:metabolism
chemical_list: D003067:Coenzymes; D019476:Insect Proteins; D008667:Metalloproteins; D000090006:Molybdenum Cofactors; D011621:Pteridines; D013466:Sulfurtransferases; C022261:molybdopterin