title: Rapid and simple determination of ciprofloxacin resistance in clinical strains of Escherichia coli.
abstract: We recently reported a simple new in situ diffusion assay, developed as a kit, to visualize DNA fragmentation in single bacterial cells. Use of this assay in a collection of 95 genetically unrelated Escherichia coli clinical strains resulted in correct identification of all of the isolates as resistant or susceptible to ciprofloxacin, consistent with the MIC results. This relevant information is obtained in 80 min.
authors: INIBIC-Complejo Hospitalario Universitario Juan Canalejo, A Coruña, Spain
authors: Rebeca
authors: R
authors: Santiso
authors: María
authors: M
authors: Tamayo
authors: José Luis
authors: JL
authors: Fernández
authors: María
authors: M
authors: del Carmen Fernández
authors: Francisca
authors: F
authors: Molina
authors: Rosa
authors: R
authors: Villanueva
authors: Jaime
authors: J
authors: Gosálvez
authors: Germán
authors: G
authors: Bou
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002939:Ciprofloxacin / Q000494:pharmacology*; D053938:DNA Fragmentation; D004269:DNA, Bacterial / Q000235:genetics; D024881:Drug Resistance, Bacterial; D004926:Escherichia coli / Q000187:drug effects* / Q000302:isolation & purification; D004927:Escherichia coli Infections / Q000382:microbiology*; D006801:Humans; D008826:Microbial Sensitivity Tests / Q000379:methods*; D012680:Sensitivity and Specificity
chemical_list: D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; D002939:Ciprofloxacin