title: Population alterations of L-arginase- and inducible nitric oxide synthase-expressed CD11b+/CD14⁻/CD15+/CD33+ myeloid-derived suppressor cells and CD8+ T lymphocytes in patients with advanced-stage non-small cell lung cancer.
abstract: BACKGROUND
  Immune aberrations have been demonstrated in tumorogenesis, and myeloid-derived suppressor cells (MDSC) have shown to play a pivotal role in mediating immune suppression in animal models of human tumors. In the present study, we explored the clinical relevance of CD11b+/CD14⁻/CD15+/CD33+ MDSCs and the association of MDSCs with CD8+ cytotoxic T lymphocytes in patients with non-small-cell lung cancer (NSCLC).
  
  
  METHODS
  The population of CD11b+/CD14⁻ cells in peripheral blood mononuclear cells (PBMNC) was determined in 173 patients with NSCLC and 42 control subjects. The expression of CD15, CD33, IL-4R, INF-γR, iNOS and L-arginase were analyzed. Cocultures with CD8+ T lymphocytes and Jurkat cells were developed to determine the impact of MDSCs on the expression of CD3ζ of CD8+ T lymphocytes.
  
  
  RESULTS
  Patients with treatment-naïve, advanced-stage NSCLC (n = 87) had an increased subpopulation of CD11b+/CD14⁻/CD15+/CD33+ cells in the PBMNCs with characteristics of MDSCs (P < 0.0001). The CD11b+/CD14⁻ cells in PBMNC also express IL-4R and INF-γR and can suppress CD3ζ expression in CD8+ T lymphocytes. The subpopulation of CD11b+/CD14⁻ cells in PBMNC was decreased in the advanced-stage NSCLC patients who had responsiveness to chemotherapy (n = 41, P < 0.0001) and in the early-stage NSCLC patients after removal of tumor (n = 8, P = 0.0391). Notably, a negative association existed between the population of CD11b+/CD14⁻ cells in PBMNC and the frequency of CD8+ T lymphocytes (n = 48, r = -0.3141, P = 0.0297).
  
  
  CONCLUSIONS
  Our study provided evidence of an increased pool of CD11b+/CD14⁻/CD15+/CD33+ MDSCs in the peripheral blood of NSCLC patients. For the suppressive effect of the cells on CD8+ T lymphocytes, these findings suggest the important role of the CD11b+/CD14⁻/CD15+/CD33+ MDSCs in mediating immunosuppression in NSCLC.
authors: Department of Thoracic Medicine, Chang Gung Memorial Hospital, and School of Medicine, Chang Gung University, No. 199, Tun-Hwa North Road, Taipei 105, Taiwan. cyliu01@adm.cgmh.org.tw
authors: Chien-Ying
authors: CY
authors: Liu
authors: Yu-Min
authors: YM
authors: Wang
authors: Chih-Liang
authors: CL
authors: Wang
authors: Po-Hao
authors: PH
authors: Feng
authors: How-Wen
authors: HW
authors: Ko
authors: Yun-Hen
authors: YH
authors: Liu
authors: Yi-Cheng
authors: YC
authors: Wu
authors: Yen
authors: Y
authors: Chu
authors: Fu-Tsai
authors: FT
authors: Chung
authors: Chih-Hsi
authors: CH
authors: Kuo
authors: Kang-Yun
authors: KY
authors: Lee
authors: Shu-Min
authors: SM
authors: Lin
authors: Horng-Chyuan
authors: HC
authors: Lin
authors: Chun-Hua
authors: CH
authors: Wang
authors: Chih-Teng
authors: CT
authors: Yu
authors: Han-Pin
authors: HP
authors: Kuo
mesh_terms: D000368:Aged; D015703:Antigens, CD / Q000276:immunology / Q000378:metabolism; D015214:Antigens, Differentiation, Myelomonocytic / Q000276:immunology / Q000378:metabolism; D001119:Arginase / Q000235:genetics / Q000378:metabolism*; D001772:Blood Cell Count; D015153:Blotting, Western; D039481:CD11b Antigen / Q000276:immunology / Q000378:metabolism; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D002289:Carcinoma, Non-Small-Cell Lung / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D002478:Cells, Cultured; D018920:Coculture Techniques; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D019169:Jurkat Cells; D007963:Leukocytes, Mononuclear / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D016256:Lewis X Antigen / Q000276:immunology / Q000378:metabolism; D018950:Lipopolysaccharide Receptors / Q000276:immunology / Q000378:metabolism; D008175:Lung Neoplasms / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D022423:Myeloid Cells / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D009367:Neoplasm Staging; D052247:Nitric Oxide Synthase Type II / Q000235:genetics / Q000378:metabolism*; D063268:Sialic Acid Binding Ig-like Lectin 3; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology / Q000378:metabolism / Q000473:pathology
chemical_list: D015703:Antigens, CD; D015214:Antigens, Differentiation, Myelomonocytic; D039481:CD11b Antigen; C571163:CD33 protein, human; D016256:Lewis X Antigen; D018950:Lipopolysaccharide Receptors; D063268:Sialic Acid Binding Ig-like Lectin 3; D052247:Nitric Oxide Synthase Type II; D001119:Arginase