title: Increasing extended-spectrum beta-lactamase production and quinolone resistance among Gram-negative bacilli causing intra-abdominal infections in the Asia/Pacific region: data from the Smart Study 2002-2006.
abstract: OBJECTIVE
  The Study for Monitoring Antimicrobial Resistance Trends (SMART) was intended to reveal the evolving profiles of antimicrobial resistance among Gram-negative pathogens causing intra-abdominal infections (IAIs).
  
  
  METHODS
  Aerobic and facultatively anaerobic Gram-negative isolates were prospectively collected from patients with IAIs in the Asia/Pacific region between 2002 and 2006. Only clinically significant isolates were included for susceptibility tests by the microdilution method. Phenotypic identification of extended-spectrum beta-lactamase (ESBL) production was performed on Escherichia coli, Klebsiella spp. and Enterobacter spp., with the measurements of MICs of cefepime alone and in the presence of clavulanic acid.
  
  
  RESULTS
  Of 7239 Gram-negative isolates, Enterobacteriaceae accounted for the majority (81.5%), and the three major species were E. coli (42.5%), Klebsiella pneumoniae (15.7%) and Pseudomonas aeruginosa (9.6%). The susceptibility rates of Enterobacteriaceae isolates to cephalosporins decreased, in parallel with the fact that the prevalence of ESBLs increased from 13% in 2002 to 28% in 2006. Among E. coli isolates, the proportion of isolates producing an ESBL varied greatly between countries, ranging from 38.6% in China to 1.2% in New Zealand, and ciprofloxacin susceptibility rate from 41.6% in China to 95.8% in New Zealand. Carbapenems and amikacin were in vitro active against more than 95% of Enterobacteriaceae isolates.
  
  
  CONCLUSIONS
  Antimicrobial resistance was frequently present among Gram-negative isolates from IAIs in the Asia/Pacific region. During the study period, there were increasing resistant rates of E. coli isolates to cephalosporins, but wide variations existed among countries. Regular updates of treatment guidelines for this region are necessary for empiric antimicrobial therapy.
authors: Department of Medicine, National Cheng Kung University Medical College and Hospital, Tainan, Taiwan.
authors: Wen-Chien
authors: WC
authors: Ko
authors: Po-Ren
authors: PR
authors: Hsueh
mesh_terms: D018784:Abdominal Abscess / Q000382:microbiology*; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001208:Asia; D044362:Australasia; D024881:Drug Resistance, Bacterial; D006090:Gram-Negative Bacteria / Q000187:drug effects* / Q000201:enzymology / Q000302:isolation & purification; D016905:Gram-Negative Bacterial Infections / Q000382:microbiology*; D006801:Humans; D008826:Microbial Sensitivity Tests / Q000379:methods; D011446:Prospective Studies; D015363:Quinolones / Q000494:pharmacology*; D001618:beta-Lactamases / Q000096:biosynthesis* / Q000145:classification; D047090:beta-Lactams / Q000494:pharmacology*
chemical_list: D000900:Anti-Bacterial Agents; D015363:Quinolones; D047090:beta-Lactams; D001618:beta-Lactamases