title: Clathrin adaptor AP1B controls adenovirus infectivity of epithelial cells.
abstract: Adenoviruses invading the organism via normal digestive or respiratory routes require the Coxsackie-adenovirus receptor (CAR) to infect the epithelial barrier cells. Because CAR is a component of tight junctions and the basolateral membrane and is normally excluded from the apical membrane, most epithelia are resistant to adenoviruses. However, we discovered that a specialized epithelium, the retinal pigment epithelium (RPE), anomalously expressed CAR at the apical surface and was highly susceptible to adenovirus infection. These properties of RPE cells correlated with the absence of the epithelial-specific clathrin adaptor AP1B. Furthermore, knockdown of this basolateral sorting adaptor in adenovirus-resistant MDCK cells promoted apical localization of CAR and increased dramatically Adenovirus infectivity. Targeting assays showed that AP1B is required for accurate basolateral recycling of CAR after internalization. AP1B knock down MDCK cells missorted CAR from recycling endosomes to the apical surface. In summary, we have characterized the cellular machinery responsible for normal sorting of an adenovirus receptor and illustrated how tissue-specific variations in such machinery result in drastic changes in tissue-susceptibility to adenoviruses.
authors: Dyson Vision Research Institute, Department of Ophthalmology, Weill Medical College of Cornell University, 1300 York Avenue, New York, NY 10065, USA.
authors: Fernando
authors: F
authors: Diaz
authors: Diego
authors: D
authors: Gravotta
authors: Ami
authors: A
authors: Deora
authors: Ryan
authors: R
authors: Schreiner
authors: John
authors: J
authors: Schoggins
authors: Erik
authors: E
authors: Falck-Pedersen
authors: Enrique
authors: E
authors: Rodriguez-Boulan
mesh_terms: D033966:Adaptor Protein Complex beta Subunits / Q000172:deficiency / Q000378:metabolism*; D000256:Adenoviridae / Q000472:pathogenicity*; D000818:Animals; D002460:Cell Line; D016764:Cell Polarity; D002966:Clathrin / Q000378:metabolism*; D062727:Coxsackie and Adenovirus Receptor-Like Membrane Protein; D004285:Dogs; D004705:Endocytosis; D004847:Epithelial Cells / Q000166:cytology / Q000378:metabolism / Q000821:virology*; D055785:Gene Knockdown Techniques; D006801:Humans; D021381:Protein Transport; D011991:Receptors, Virus / Q000378:metabolism; D055213:Retinal Pigment Epithelium / Q000166:cytology / Q000378:metabolism / Q000821:virology; D019108:Tight Junctions
chemical_list: D033966:Adaptor Protein Complex beta Subunits; C568771:CLMP protein, human; D002966:Clathrin; D062727:Coxsackie and Adenovirus Receptor-Like Membrane Protein; D011991:Receptors, Virus