The influence of IgE-enhancing and IgE-suppressive gammadelta T cells changes with exposure to inhaled ovalbumin.
It has been reported that the IgE response to allergens is influenced by gammadelta T cells. Intrigued by a study showing that airway challenge of mice with OVA induces in the spleen the development of gammadelta T cells that suppress the primary IgE response to i.p.-injected OVA-alum, we investigated the gammadelta T cells involved. We found that the induced IgE suppressors are contained within the Vgamma4(+) subset of gammadelta T cells of the spleen, that they express Vdelta5 and CD8, and that they depend on IFN-gamma for their function. However, we also found that normal nonchallenged mice harbor IgE-enhancing gammadelta T cells, which are contained within the larger Vgamma1(+) subset of the spleen. In cell transfer experiments, airway challenge of the donors was required to induce the IgE suppressors among the Vgamma4(+) cells. Moreover, this challenge simultaneously turned off the IgE enhancers among the Vgamma1(+) cells. Thus, airway allergen challenge differentially affects two distinct subsets of gammadelta T cells with nonoverlapping functional potentials, and the outcome is IgE suppression.
Huang
Yafei
Y
Integrated Department of Immunology, National Jewish Health, Denver, CO 80206, USA.
Jin
Niyun
N
Roark
Christina L
CL
Aydintug
M Kemal
MK
Wands
J M
JM
Huang
Hua
H
O'Brien
Rebecca L
RL
Born
Willi K
WK
D000280:Administration, Inhalation; D000485:Allergens / Q000008:administration & dosage / Q000494:pharmacology; D000534:Alum Compounds / Q000008:administration & dosage / Q000494:pharmacology*; D000818:Animals; D015536:Down-Regulation / Q000276:immunology; D007073:Immunoglobulin E / Q000032:analysis*; D007371:Interferon-gamma / Q000172:deficiency / Q000276:immunology; D051379:Mice; D018345:Mice, Knockout; D010047:Ovalbumin / Q000008:administration & dosage / Q000494:pharmacology*; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000276:immunology*; D013154:Spleen / Q000166:cytology; D016176:T-Lymphocyte Subsets / Q000276:immunology
D000485:Allergens; D000534:Alum Compounds; D016692:Receptors, Antigen, T-Cell, gamma-delta; C446402:ovalbumin-alum; D007073:Immunoglobulin E; D007371:Interferon-gamma; D010047:Ovalbumin