High heterogeneity of plasma membrane microfluidity in multidrug-resistant cancer cells.
Diffusion-time distribution analysis (DDA) has been used to explore the plasma membrane fluidity of multidrug-resistant cancer cells (LR73 carcinoma cells) and also to characterize the influence of various membrane agents present in the extracellular medium. DDA is a recent single-molecule technique, based on fluorescence correlation spectroscopy (FCS), well suited to retrieve local organization of cell membrane. The method was conducted on a large number of living cells, which enabled us to get a detailed overview of plasma membrane microviscosity, and plasma membrane micro-organization, between the cells of the same line. Thus, we clearly reveal the higher heterogeneity of plasma membrane in multidrug-resistant cancer cells in comparison with the nonresistant ones (denoted sensitive cells). We also display distinct modifications related to a membrane fluidity modulator, benzyl alcohol, and two revertants of multidrug resistance, verapamil and cyclosporin-A. A relation between the distribution of the diffusion-time values and the modification of membrane lateral heterogeneities is proposed.
Boutin
Céline
C
Universite de Technologie de Troyes, Laboratoire de Nanotechnologie et d'Instrumentation Optique, LRC CEA, Institut Charles Delaunay, FRE CNRS 2848, 12 rue Marie Curie, BP 2060, Troyes Cedex 10010, France.
Roche
Yann
Y
Millot
Christine
C
Deturche
Régis
R
Royer
Pascal
P
Manfait
Michel
M
Plain
Jéro Me
JM
Jeannesson
Pierre
P
Millot
Jean-Marc
JM
Jaffiol
Rodolphe
R
D000465:Algorithms; D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D019905:Benzyl Alcohol / Q000494:pharmacology; D016466:CHO Cells; D045744:Cell Line, Tumor; D002462:Cell Membrane / Q000737:chemistry* / Q000187:drug effects; D006224:Cricetinae; D003412:Cricetulus; D016572:Cyclosporine / Q000494:pharmacology; D004058:Diffusion; D018432:Drug Resistance, Multiple; D019008:Drug Resistance, Neoplasm; D004867:Equipment Design; D005260:Female; D008563:Membrane Lipids / Q000737:chemistry; D051379:Mice; D044085:Microfluidics / Q000379:methods*; D008954:Models, Biological; D010051:Ovarian Neoplasms / Q000737:chemistry* / Q000188:drug therapy*; D013050:Spectrometry, Fluorescence / Q000379:methods*; D018709:Statistics, Nonparametric; D013997:Time Factors; D014700:Verapamil / Q000494:pharmacology; D014783:Viscosity
D000970:Antineoplastic Agents; D008563:Membrane Lipids; D016572:Cyclosporine; D014700:Verapamil; D019905:Benzyl Alcohol