Sequence dependent femtosecond-resolved hydration dynamics in the minor groove of DNA and histone-DNA complexes.
Understanding the sequence dependent molecular recognition of DNA is crucial for the rational design of many drugs. Femtosecond resolved studies on the hydration dynamics of the dodecamer duplexes having sequences (CGCGAATTCGCG)2 and (CGCAAATTTGCG)2, and their complexes with the nucleic protein histone 1 (H1) reveal significant correlation of the molecular recognition of the DNA and DNA-protein complexes with the dynamics of hydration. The different molecular recognition of DNA and DNA-protein complexes is also borne out by circular dichroism (CD) and fluorescence detected CD measurements.
Banerjee
Debapriya
D
Unit for Nano Science & Technology, Department of Chemical, Biological & Macromolecular Sciences, S. N. Bose National Centre for Basic Sciences, Block JD, Sector III, Salt Lake, Kolkata 700 098, India.
Makhal
Abhinandan
A
Pal
Samir Kumar
SK
D000818:Animals; D001483:Base Sequence; D006690:Bisbenzimidazole / Q000737:chemistry; D002417:Cattle; D002942:Circular Dichroism / Q000379:methods; D004247:DNA / Q000737:chemistry* / Q000378:metabolism; D005453:Fluorescence; D005454:Fluorescence Polarization; D006657:Histones / Q000737:chemistry* / Q000378:metabolism; D007700:Kinetics; D009690:Nucleic Acid Conformation; D012399:Rotation; D014867:Water / Q000737:chemistry*
D006657:Histones; D014867:Water; D004247:DNA; D006690:Bisbenzimidazole