A detailed microscopic study of the changes in the aorta of experimental model of postmenopausal rats fed with repeatedly heated palm oil.
Hypercholesterolaemia, increase in lipid peroxidation and hyperhomocysteinaemia may contribute to the pathogenesis of atherosclerosis. This study was performed to examine the effects of repeatedly heated palm oil mixed with 2% cholesterol diet on atherosclerosis in oestrogen-deficient postmenopausal rats. Ovariectomy causes disruption of tunica intima layer of the rat aorta simulating a postmenopausal condition in females. Twenty-four ovariectomized female Sprague-Dawley rats were divided into four groups. The control group received 2% cholesterol diet without palm oil. A diet with 2% cholesterol content fortified with fresh, once-heated and five-times-heated palm oil was given to the other treatment groups. The rats were sacrificed at the end of 4 months of study and the aortic arch tissue was processed for histomorphometry and electron microscopy. On observation, there was disruption of the intimal layer of the ovariectomized rat aorta. There was no obvious ultrastructural change in the aorta of the rats fed with fresh palm oil. The ultrastructural changes were minimal with once-heated palm oil, in which there was a focal disruption of the endothelial layer. The focal disruption was more pronounced with five-times-heated palm oil. The results of this study show that the ingestion of fresh palm oil may have a protective effect on the aorta but such a protective action may be lost when the palm oil is repeatedly heated. The study may be clinically important for all postmenopausal women who are susceptible to atherosclerosis.
Adam
Siti Khadijah
SK
Department of Pharmacology, Faculty of Medicine, Universiti Kebangsaan Malaysia, Kuala Lumpur, Malaysia.
Das
Srijit
S
Jaarin
Kamsiah
K
D000818:Animals; D001013:Aorta, Thoracic / Q000187:drug effects* / Q000648:ultrastructure; D050197:Atherosclerosis / Q000209:etiology* / Q000473:pathology; D004195:Disease Models, Animal; D004435:Eating / Q000187:drug effects; D004967:Estrogens / Q000172:deficiency; D005260:Female; D006358:Hot Temperature; D010052:Ovariectomy; D000073878:Palm Oil; D010938:Plant Oils / Q000633:toxicity*; D017698:Postmenopause; D051381:Rats; D017207:Rats, Sprague-Dawley; D017539:Tunica Intima / Q000648:ultrastructure; D017540:Tunica Media / Q000648:ultrastructure; D015430:Weight Gain / Q000187:drug effects
D004967:Estrogens; D010938:Plant Oils; D000073878:Palm Oil