Automation in high-content flow cytometry screening.
High-content flow cytometric screening (FC-HCS) is a 21st Century technology that combines robotic fluid handling, flow cytometric instrumentation, and bioinformatics software, so that relatively large numbers of flow cytometric samples can be processed and analysed in a short period of time. We revisit a recent application of FC-HCS to the problem of cellular signature definition for acute graft-versus-host-disease. Our focus is on automation of the data processing steps using recent advances in statistical methodology. We demonstrate that effective results, on par with those obtained via manual processing, can be achieved using our automatic techniques. Such automation of FC-HCS has the potential to drastically improve diagnosis and biomarker identification.
Naumann
U
U
School of Mathematics and Statistics, The University of New South Wales, Sydney, Australia.
Wand
M P
MP
D000208:Acute Disease; D001331:Automation / Q000295:instrumentation* / Q000379:methods; D016913:Blood Component Transfusion; D016026:Bone Marrow Transplantation; D019295:Computational Biology; D005434:Flow Cytometry / Q000295:instrumentation* / Q000379:methods; D006086:Graft vs Host Disease / Q000097:blood / Q000473:pathology*; D006801:Humans; D015203:Reproducibility of Results; D012371:Robotics / Q000295:instrumentation* / Q000379:methods