[Study on the biofilm early formation of Streptococcus mutans luxS gene mutation].
OBJECTIVE
To investigate the effect of Streptococcus mutans luxS mutarotation on the early biofilm formation.


METHODS
Based on the immobilization of magnetic beads by adherent cells, an assay of biofilm quantitative analysis was developed for the kinetic quantification of biofilm formation in this study. Streptococcus mutans luxS mutant strain was constructed and subject to this biofilm luxS mutant strain were compared.


RESULTS
The delta luxS mutant started to form a biofilm from the 6th hour (delta BFI = 2.015), and the delta BFI of luxS mutant increased more quickly than that of the wild type strain, until reaching a complete immobilization of the beads after 10 hours (delta BFI = 7.025). The wild-type strain start to form a biofilm from the 10 th hour (delta BFI = 1.875) and the beads were completely immobilized between 12 and 14 hours.


CONCLUSIONS
The luxS mutation can accelerate biofilm on a polystyrene surface during the mid-exponential growth phase. And a luxS-dependent signal may play an important role in the early biofilm formation of Streptococcus mutans.
Huang
Zheng-wei
ZW
Dept. of Cariology and Endodontology, Ninth People's Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine & Shanghai Research Insitute of Stomatology, Shanghai, China huangzhengwei@shsmu.edu.cn
Liu
Zheng
Z
Tang
Zi-sheng
ZS
Ma
Rui
R
Zhu
Cai-lian
CL
D001426:Bacterial Proteins / Q000235:genetics*; D018441:Biofilms / Q000254:growth & development; D013437:Carbon-Sulfur Lyases / Q000235:genetics*; D017353:Gene Deletion; D015964:Gene Expression Regulation, Bacterial; D013295:Streptococcus mutans / Q000235:genetics* / Q000254:growth & development
D001426:Bacterial Proteins; D013437:Carbon-Sulfur Lyases; C117753:LuxS protein, Bacteria