Change of peripheral blood mononuclear cells IFN-gamma, IL-10, and TGF-beta1 mRNA expression levels with active human cytomegalovirus infection in orthotopic liver transplantation.
OBJECTIVE
To analyze the expression levels of interferon gamma (IFN-gamma), interleukin 10 (IL-10), and transforming growth factor beta 1 (TGF-beta1) mRNA in peripheral blood mononuclear cells (PBMCs) in liver transplanted recipients with active HCMV infection.


METHODS
PBMCs were isolated from 20 liver transplanted recipients with active HCMV infection and 20 recipients without HCMV infection. The expression levels of IFN-gamma, IL-10 and TGF-beta1 mRNA in PBMCs were measured by TaqMan real-time reverse transcriptase-polymerase chain reaction (RT-PCR). The results were compared with that from 20 healthy individuals.


RESULTS
The expression level of TGF-beta1 mRNA was significantly increased in the active HCMV infection group compared with that in stable group or healthy group (P < .001). The expression level of IL-10 mRNA was significantly increased in the active HCMV infection group compared with the healthy group (P = .001). However, the IFN-gamma mRNA expression level was significantly decreased in the active HCMV infection group compared with that in the stable group or the healthy group (P < .05).


CONCLUSIONS
Cytokine production plays a role in the HCMV infection. This may provide an important clue to a better understanding of the pathogenesis in liver transplanted recipients with active HCMV infection.
Zhang
Y
Y
Department of Anaesthesia, Tianjin Medical University Cancer Institute and Hospital, Tianjin, China.
Wang
Y-L
YL
Liu
Y-W
YW
Li
Q
Q
Yuan
Y-H
YH
Niu
W-Y
WY
Sun
L-Y
LY
Zhu
Z-J
ZJ
Shen
Z-Y
ZY
Han
R-F
RF
D000199:Actins / Q000235:genetics; D000328:Adult; D003586:Cytomegalovirus Infections / Q000235:genetics* / Q000276:immunology*; D017931:DNA Primers; D018076:DNA, Complementary / Q000235:genetics; D005260:Female; D005786:Gene Expression Regulation / Q000276:immunology; D006801:Humans; D007371:Interferon-gamma / Q000235:genetics*; D016753:Interleukin-10 / Q000235:genetics*; D016031:Liver Transplantation / Q000009:adverse effects* / Q000276:immunology; D008297:Male; D008875:Middle Aged; D012333:RNA, Messenger / Q000235:genetics*; D053773:Transforming Growth Factor beta1 / Q000235:genetics*
D000199:Actins; D017931:DNA Primers; D018076:DNA, Complementary; D012333:RNA, Messenger; D053773:Transforming Growth Factor beta1; D016753:Interleukin-10; D007371:Interferon-gamma