title: Applications of nucleic acid chaperone activity of CspA and its homologues.
abstract: In Escherichia coli, the cold shock response is exerted upon temperature change from 37 to 15 degrees C and is characterized by induction of several cold shock proteins including its major cold shock protein, CspA. E. coli CspA family consists of nine members, CspA to CspI. CspA and some of its homologues play a critical role in cold acclimation of cells as RNA chaperones by destabilizing secondary structures in RNAs. Here, we showed that the nucleic acid melting activity of Csp proteins can be used to facilitate reactions, such as RT-PCR or RNA cleavage reactions by endoribonucleases, which are hindered by presence of secondary structures in the DNA/RNA substrate used. The low substrate specificity of Csps together with their compatibility with various enzymes and their stability and activity over a broad temperature range makes them ideal candidates to be used for a variety of processes.
authors: Department of Biochemistry, Robert Wood Johnson Medical School, Piscataway, NJ 08854, USA. phadtasa@umdnj.edu
authors: Sangita
authors: S
authors: Phadtare
authors: Ling
authors: L
authors: Zhu
authors: Takashi
authors: T
authors: Uemori
authors: Hiroyuki
authors: H
authors: Mukai
authors: Ikunoshin
authors: I
authors: Kato
authors: Masayori
authors: M
authors: Inouye
mesh_terms: D057773:Cold Shock Proteins and Peptides; D003080:Cold Temperature; D004722:Endoribonucleases / Q000378:metabolism; D004926:Escherichia coli / Q000502:physiology*; D029968:Escherichia coli Proteins / Q000378:metabolism*; D006360:Heat-Shock Proteins / Q000378:metabolism*; D006801:Humans; D018832:Molecular Chaperones / Q000378:metabolism*; D009696:Nucleic Acids / Q000378:metabolism*; D012194:RNA-Directed DNA Polymerase / Q000378:metabolism; D013312:Stress, Physiological; D013379:Substrate Specificity
chemical_list: D057773:Cold Shock Proteins and Peptides; C498192:CspA protein, E coli; D029968:Escherichia coli Proteins; D006360:Heat-Shock Proteins; D018832:Molecular Chaperones; D009696:Nucleic Acids; D012194:RNA-Directed DNA Polymerase; D004722:Endoribonucleases