Clarithromycin susceptibility testing of Mycobacterium avium complex using 2,3-diphenyl-5-thienyl-(2)-tetrazolium chloride microplate assay with Middlebrook 7H9 broth.
A series of 119 Mycobacterium avium complex isolates were subjected to clarithromycin susceptibility testing using microplates containing 2,3-diphenyl-5-thienyl-(2)-tetrazolium chloride (STC). Among 119 isolates, 114 (95.8%) were susceptible to clarithromycin and 5 were resistant according to the new and the standard method. STC counts the low cost and reduces the number of procedures needed for susceptibility testing.
Park
Young Kil
YK
Korean Institute of Tuberculosis, Seoul, Korea.
Koh
Won-Jung
WJ
Kim
Shin Ok
SO
Shin
Sonya
S
Kim
Bum Joon
BJ
Cho
Sang-Nae
SN
Lee
Sun Min
SM
Chang
Chulhun L
CL
D017291:Clarithromycin / Q000494:pharmacology*; D003470:Culture Media; D006801:Humans; D008826:Microbial Sensitivity Tests / Q000379:methods*; D015269:Mycobacterium avium Complex / Q000187:drug effects* / Q000302:isolation & purification; D013778:Tetrazolium Salts / Q000737:chemistry*
C494145:2,3-diphenyl-5-thienyl-(2)-tetrazolium; D003470:Culture Media; D013778:Tetrazolium Salts; D017291:Clarithromycin