The isolation of scFvs against small target molecules.
Phage display has the capacity to rapidly isolate recombinant antibodies against protein targets and other molecules of significant size. However, there is no obvious lower limit to the power of the selection methods: this chapter describes how the techniques of phage display can be adapted to allow the isolation of antibodies against very small compounds. Antibodies generated in this way have many uses including the detection and quantitative analysis of the target chemical moiety in samples such as foods, water, and body fluids.
Shaw
Iain
I
National Centre for Biomedical Engineering Science, National University of Ireland - Galway, Galway, Ireland.
Kane
Marian
M
D000911:Antibodies, Monoclonal / Q000276:immunology*; D001435:Bacteriophages / Q000276:immunology; D015374:Biosensing Techniques / Q000379:methods*; D006241:Haptens / Q000276:immunology*; D006801:Humans; D007135:Immunoglobulin Variable Region / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D019151:Peptide Library
D000911:Antibodies, Monoclonal; D006241:Haptens; D007135:Immunoglobulin Variable Region; D019151:Peptide Library