title: Intranasal administration of proteoliposome-derived cochleates from Vibrio cholerae O1 induce mucosal and systemic immune responses in mice.
abstract: Conservative estimates place the death toll from cholera at more than 100,000 persons each year. A particulate mucosal vaccine strategy combining antigens and immune stimulator molecules from Vibrio cholerae to overcome this problem is described. Proteoliposomes extracted from V. cholerae O1 were transformed into cochleates (AFCo2, Adjuvant Finlay cochleate 2) through a calcium inducible rotary dialysis method. Light microscopy was carried out and tubules of 16.25+/-4.57 microm in length were observed. Western blots were performed to verify the immunochemical properties of the main AFCo2 incorporated antigens, revealing full recognition of the outer membrane protein U (OmpU), lipopolysaccharide (LPS), and mannose-sensitive hemagglutinin (MSHA) antigens. AFCo2 were administered by the intranasal route using a two or three dose schedule and the immune response against V. cholerae antigens was assessed. Three AFCo2 doses were required to induce significant (p<0.05), antigen specific IgA in saliva (1.34+/-0.135) and feces (0.60+/-0.089). While, two or three doses of AFCo2 or proteoliposomes induce similar specific IgG and vibriocidal activity responses in sera. These results show for the first time that AFCo2 can be obtained from V. cholerae O1 proteoliposomes and have the potential to protect against the pathogen when administered intranasally.
authors: Immunology Department, Bacterial Vaccines Division of Finlay Institute, Havana, Cuba. racevedo@finlay.edu.cu
authors: Reinaldo
authors: R
authors: Acevedo
authors: Adriana
authors: A
authors: Callicó
authors: Judith
authors: J
authors: del Campo
authors: Elizabeth
authors: E
authors: González
authors: Bárbara
authors: B
authors: Cedré
authors: Lissette
authors: L
authors: González
authors: Belkis
authors: B
authors: Romeu
authors: Caridad
authors: C
authors: Zayas
authors: Miriam
authors: M
authors: Lastre
authors: Sonsire
authors: S
authors: Fernández
authors: Reynaldo
authors: R
authors: Oliva
authors: Luis
authors: L
authors: García
authors: José Luis
authors: JL
authors: Pérez
authors: Oliver
authors: O
authors: Pérez
mesh_terms: D000281:Administration, Intranasal; D000818:Animals; D003840:Deoxycholic Acid / Q000008:administration & dosage / Q000276:immunology; D004492:Edetic Acid / Q000008:administration & dosage / Q000276:immunology; D005260:Female; D018928:Immunity, Mucosal / Q000187:drug effects / Q000276:immunology; D007074:Immunoglobulin G / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D009092:Mucous Membrane / Q000187:drug effects / Q000276:immunology*; D011510:Proteolipids / Q000008:administration & dosage* / Q000276:immunology*; D041241:Vibrio cholerae O1 / Q000276:immunology*
chemical_list: D007074:Immunoglobulin G; D011510:Proteolipids; C020485:proteoliposomes; D003840:Deoxycholic Acid; D004492:Edetic Acid