[Analysis of variant translocation der ins (17; 15) in patient with APL by G-banding technique and interphase fluorescence in situ hybridization].
To investigate the biological characteristics of the variant translocation der ins (17;15) in a patient with acute promyelocytic leukemia (APL), the conventional G-banding technique, interphase fluorescence in situ hybridization (int-FISH), RT-PCR, gene scanning, gene sequence and flow cytometry were performed. The results indicated that the variant translocation der ins (17, 15) observed by G banding technique was a rare type, the int-FISH assay by using dual-color pml/raralpha fusion probes confirmed the cytogenetic findings. The detection results of other molecular methods demonstrated the existence of the whole pml/raralpha fusion gene, while this case had insertion variant translocation. This patient got complete remission by using combined chemotherapy, and survives with continuous complete remission during following up for 1 year. In conclusion, the variant translocation der ins (17; 15) is rare type in APL, its incidence is lower, several signal types in detection of int-FISH were observed and the combination chemotherapy for this patient showed more obvious efficacy.
Wang
Tong
T
Laboratory of Cytogenetics, Specific Examination Center, Beijing Daopei Hospital, Beijing, China.
Qiu
Jing-Ying
JY
Yu
Chun-Fu
CF
Ma
Xiao-Lan
XL
Jia
Xiao-Peng
XP
Wang
Yan-Ping
YP
Liu
Hong-Xing
HX
Lin
Yue-Hui
YH
Tong
Chun-Rong
CR
Lu
Dao-Pei
DP
D002871:Chromosome Banding; D002884:Chromosomes, Human, Pair 15; D002886:Chromosomes, Human, Pair 17; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D007399:Interphase / Q000235:genetics; D015473:Leukemia, Promyelocytic, Acute / Q000235:genetics*; D008297:Male; D014178:Translocation, Genetic; D055815:Young Adult