Effect of 6-day intense Kendo training on lymphocyte counts and its expression of CD95.
This study examines the effects of 6-day intensive training on lymphocyte counts and their expression of CD95. Eight healthy Kendo athletes underwent 6-day Kendo training of about 310 min each day. Blood samples were collected at 2 weeks before (PRE), the first day (Day 1), third day (Day 3), fifth day (Day 5), and 1 week after the training period (POST) to determine lymphocyte counts and CD95 expression on CD95 lymphocytes (CD4(+), CD8(+)) using flow cytometry. The total lymphocyte counts were significantly lower at Day 3 than at PRE. The CD8(+) cell counts were significantly lower at Day 3 than at PRE. The percentage of CD95(+) lymphocytes was significantly higher at Day 1 and Day 3 than at PRE. The percentage of CD8(+)CD95(+) cells did not change significantly. The total lymphocyte counts decreased and a concomitant increase of CD95(+) lymphocyte was observed, whereas the decrease in CD8(+) cell counts was not associated with the increase in CD8(+)CD95(+) cells. Therefore, short-term high-intensity exercise induced a decrease in the T lymphocyte counts without increasing in CD95(+) expression.
Tanimura
Yuko
Y
Graduate School of Comprehensive Human Sciences, University of Tsukuba, Tennodai 1-1-1, Tsukuba, Ibaraki, 305-8574, Japan. tanimura@koto.kpu-m.ac.jp
Kon
Michihiro
M
Shimizu
Kazuhiro
K
Kimura
Fuminori
F
Kono
Ichiro
I
Ajisaka
Ryuichi
R
D000293:Adolescent; D006801:Humans; D006854:Hydrocortisone / Q000097:blood; D018655:Lymphocyte Count; D016139:Martial Arts / Q000502:physiology*; D010806:Physical Education and Training / Q000379:methods*; D005082:Physical Exertion / Q000502:physiology; D013601:T-Lymphocytes / Q000166:cytology* / Q000378:metabolism*; D013997:Time Factors; D055815:Young Adult; D019014:fas Receptor / Q000378:metabolism*
D019014:fas Receptor; D006854:Hydrocortisone