An operationally simple colorimetric assay of hyaluronidase activity using cationic gold nanoparticles.
An operationally simple colorimetric way for measuring hyaluronidase activity was developed using cysteamine-bound gold nanoparticles. The addition of gold nanoparticles into hyaluronidase-containing solutions resulted in color changes, which could easily be observed with the naked eye or a UV/Vis spectrophotometer.
Kim
Jung-Won
JW
BioNanotechnology Research Center, Korea Research Institute of Bioscience and Biotechnology, P.O. Box 115, Yuseong-gu, Daejeon 305-600, South Korea.
Kim
Joong Hyun
JH
Chung
Sang Jeon
SJ
Chung
Bong Hyun
BH
D000042:Absorption; D003124:Colorimetry / Q000379:methods*; D006046:Gold / Q000737:chemistry*; D006821:Hyaluronoglucosaminidase / Q000032:analysis / Q000378:metabolism*; D053768:Metal Nanoparticles / Q000737:chemistry*; D013997:Time Factors
D006046:Gold; D006821:Hyaluronoglucosaminidase