title: Accurate molecular weight analysis of histones using FFE and RP-HPLC on monolithic capillary columns.
abstract: Due to their large diversity with respect to post-translational modifications (PTMs), the family of histones provides a major analytical challenge in current proteomics research. Their function has a large impact on the transcription of DNA, and as a result, on the expression of proteins. The variation in PTMs regulates transcription, and, as a result, many methods are being employed for the in-depth analysis of histones. In this paper, we present a separation strategy for histones based on free-flow electrophoresis (FFE) followed by an RP separation on capillary monolithic PS-DVB columns. The capillary columns are directly interfaced with an FT-ICR MS providing an online system for the detection and accurate molecular weight analysis of intact histones.
authors: Dionex Corporation, Amsterdam, The Netherlands.
authors: Evert-Jan
authors: EJ
authors: Sneekes
authors: Jun
authors: J
authors: Han
authors: Monica
authors: M
authors: Elliot
authors: Juan
authors: J
authors: Ausio
authors: Remco
authors: R
authors: Swart
authors: Albert J R
authors: AJ
authors: Heck
authors: Christoph
authors: C
authors: Borchers
mesh_terms: D000818:Animals; D002417:Cattle; D002645:Chickens; D002853:Chromatography, Liquid / Q000295:instrumentation / Q000379:methods; D019075:Electrophoresis, Capillary / Q000295:instrumentation / Q000379:methods; D006657:Histones / Q000032:analysis / Q000302:isolation & purification; D013058:Mass Spectrometry / Q000295:instrumentation / Q000379:methods; D008970:Molecular Weight
chemical_list: D006657:Histones