Guided selection methods through chain shuffling.
We provide procedures for the panning of fully humanized Fab antibodies using guided selection. Human heavy and light chain genes are amplified. A parental light chain is cloned into a phage display vector and combined with the heavy chain library. After several rounds of panning, positive clones are identified and the heavy chain sequences that are recovered are combined with light chains for further selection by phage display. Human Fab antibodies are obtained that bind the same epitope as the parental antibody.
Guo-Qiang
Bao
B
Department of General Surgery, Tangdu Hospital, Xi'an 710038, Shaanxi Province, China. guoqiang_bao@hotmail.com
Xian-Li
He
H
D006801:Humans; D007140:Immunoglobulin Fab Fragments / Q000235:genetics* / Q000276:immunology / Q000302:isolation & purification*; D007143:Immunoglobulin Heavy Chains / Q000235:genetics* / Q000276:immunology; D007147:Immunoglobulin Light Chains / Q000235:genetics* / Q000276:immunology; D019151:Peptide Library; D011993:Recombinant Fusion Proteins / Q000235:genetics* / Q000276:immunology
D007140:Immunoglobulin Fab Fragments; D007143:Immunoglobulin Heavy Chains; D007147:Immunoglobulin Light Chains; D019151:Peptide Library; D011993:Recombinant Fusion Proteins