title: Development and validation of a sensitive and selective method using GC/MS-MS for quantification of 5-fluorouracil in hospital wastewater.
abstract: Pollution of the environment by pharmaceuticals is a subject of growing scientific and societal concern. However, few quantitative data have been reported concerning hospital wastewater contamination. Among the different molecules used at hospital, antineoplastic drugs appear to be of special interest, and 5-fluorouracil (5-FU) can be considered as a key compound of this therapeutic class. To monitor this pharmaceutical in hospital wastewater, a highly specific and selective method was developed using gas chromatography tandem mass spectrometry after solid-phase extraction. This sensitive method (limit of quantification = 40 ng L(-1)) was then applied to assess sewage contamination of a middle-size hospital with oncology service located in Paris, France. Native 5-FU was detectable in 12 of the 14 analysed samples. In positive samples, concentration range was measured from 0.09 to 4.0 microg L(-1). Finally, a predicting model for the hospital wastewater concentrations is presented, and results of this model are discussed.
authors: Laboratoire Santé Publique-Environnement, Université Paris Sud 11-Faculté de Pharmacie, 5 rue J.B. Clément, 92296 Chatenay-Malabry, France. jumullot@aol.com
authors: Jean-Ulrich
authors: JU
authors: Mullot
authors: Sara
authors: S
authors: Karolak
authors: Anne
authors: A
authors: Fontova
authors: Bruno
authors: B
authors: Huart
authors: Yves
authors: Y
authors: Levi
mesh_terms: D000964:Antimetabolites, Antineoplastic / Q000032:analysis*; D005472:Fluorouracil / Q000032:analysis*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006761:Hospitals; D008956:Models, Chemical; D012680:Sensitivity and Specificity; D052616:Solid Phase Extraction / Q000379:methods; D014865:Waste Disposal, Fluid; D014867:Water / Q000032:analysis*; D014874:Water Pollutants, Chemical / Q000032:analysis*
chemical_list: D000964:Antimetabolites, Antineoplastic; D014874:Water Pollutants, Chemical; D014867:Water; D005472:Fluorouracil