Improved phase sensitivity in spectral domain phase microscopy using line-field illumination and self phase-referencing.
We report a quantitative phase microscope based on spectral domain optical coherence tomography and line-field illumination. The line illumination allows self phase-referencing method to reject common-mode phase noise. The quantitative phase microscope also features a separate reference arm, permitting the use of high numerical aperture (NA > 1) microscope objectives for high resolution phase measurement at multiple points along the line of illumination. We demonstrate that the path-length sensitivity of the instrument can be as good as 41 pm/square root of Hz, which makes it suitable for nanometer scale study of cell motility. We present the detection of natural motions of cell surface and two-dimensional surface profiling of a HeLa cell.
Yaqoob
Zahid
Z
G. R. Harrison Spectroscopy Laboratory, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA.
Choi
Wonshik
W
Oh
Seungeun
S
Lue
Niyom
N
Park
Yongkeun
Y
Fang-Yen
Christopher
C
Dasari
Ramachandra R
RR
Badizadegan
Kamran
K
Feld
Michael S
MS
D002462:Cell Membrane / Q000378:metabolism; D004867:Equipment Design; D006367:HeLa Cells; D006801:Humans; D007834:Lasers; D008853:Microscopy / Q000295:instrumentation / Q000379:methods*; D009474:Neurons / Q000473:pathology; D055095:Optics and Photonics; D013053:Spectrophotometry / Q000379:methods; D013499:Surface Properties; D041623:Tomography, Optical Coherence / Q000379:methods*