Real-time monitoring of odorant-induced cellular reactions using surface plasmon resonance.
Surface plasmon resonance (SPR) is a powerful technique for measuring molecular interaction in real-time. SPR can be used to detect molecule to cell interactions as well as molecule to molecule interactions. In this study, the SPR-based biosensing technique was applied to real-time monitoring of odorant-induced cellular reactions. An olfactory receptor, OR I7, was fused with a rho-tag import sequence at the N-terminus of OR I7, and expressed on the surface of human embryonic kidney (HEK)-293 cells. These cells were then immobilized on a SPR sensor chip. The intensity of the SPR response was linearly dependent on the amount of injected odorant. Among all the aldehyde containing odorants tested, the SPR response was specifically high for octanal, which is the known cognate odorant for the OR I7. This SPR response is believed to have resulted from intracellular signaling triggered by the binding of odorant molecules to the olfactory receptors expressed on the cell surface. This SPR system combined with olfactory receptor-expressed cells provides a new olfactory biosensor system for selective and quantitative detection of volatile compounds.
Lee
Sang Hun
SH
School of Chemical and Biological Engineering, Institute of Bioengineering, Seoul National University, Gwanak-Gu, Sillim-Dong, San 56-1, Seoul, 151-744, Republic of Korea.
Ko
Hwi Jin
HJ
Park
Tai Hyun
TH
D000818:Animals; D002460:Cell Line; D006801:Humans; D009812:Odorants / Q000032:analysis*; D011485:Protein Binding; D051381:Rats; D018035:Receptors, Odorant / Q000235:genetics / Q000378:metabolism*; D012680:Sensitivity and Specificity; D020349:Surface Plasmon Resonance / Q000379:methods*; D014162:Transfection
D018035:Receptors, Odorant; C509506:olfactory receptor I7, rat