Diffuse optical monitoring of hemodynamic changes in piglet brain with closed head injury.
We used a nonimpact inertial rotational model of a closed head injury in neonatal piglets to simulate the conditions following traumatic brain injury in infants. Diffuse optical techniques, including diffuse reflectance spectroscopy and diffuse correlation spectroscopy (DCS), were used to measure cerebral blood oxygenation and blood flow continuously and noninvasively before injury and up to 6 h after the injury. The DCS measurements of relative cerebral blood flow were validated against the fluorescent microsphere method. A strong linear correlation was observed between the two techniques (R=0.89, p<0.00001). Injury-induced cerebral hemodynamic changes were quantified, and significant changes were found in oxy- and deoxy-hemoglobin concentrations, total hemoglobin concentration, blood oxygen saturation, and cerebral blood flow after the injury. The diffuse optical measurements were robust and also correlated well with recordings of vital physiological parameters over the 6-h monitoring period, such as mean arterial blood pressure, arterial oxygen saturation, and heart rate. Finally, the diffuse optical techniques demonstrated sensitivity to dynamic physiological events, such as apnea, cardiac arrest, and hypertonic saline infusion. In total, the investigation corraborates potential of the optical methods for bedside monitoring of pediatric and adult human patients in the neurointensive care unit.
Zhou
Chao
C
University of Pennsylvania, Department of Physics, 209 South 33rd Street, Philadelphia, Pennsylvania 19104, USA.
Eucker
Stephanie A
SA
Durduran
Turgut
T
Yu
Guoqiang
G
Ralston
Jill
J
Friess
Stuart H
SH
Ichord
Rebecca N
RN
Margulies
Susan S
SS
Yodh
Arjun G
AG
D000704:Analysis of Variance; D000818:Animals; D000831:Animals, Newborn; D001049:Apnea / Q000503:physiopathology; D001921:Brain / Q000098:blood supply* / Q000378:metabolism / Q000503:physiopathology; D001930:Brain Injuries / Q000378:metabolism / Q000503:physiopathology*; D004195:Disease Models, Animal; D005260:Female; D005453:Fluorescence; D016489:Head Injuries, Closed / Q000378:metabolism / Q000503:physiopathology*; D006323:Heart Arrest / Q000503:physiopathology; D006454:Hemoglobins / Q000032:analysis; D016014:Linear Models; D055095:Optics and Photonics / Q000379:methods*; D010092:Oximetry; D010100:Oxygen / Q000032:analysis / Q000097:blood; D010108:Oxyhemoglobins / Q000032:analysis; D015203:Reproducibility of Results; D019265:Spectroscopy, Near-Infrared / Q000379:methods; D013057:Spectrum Analysis / Q000379:methods*; D013552:Swine
D006454:Hemoglobins; D010108:Oxyhemoglobins; C100003:deoxyhemoglobin; D010100:Oxygen