title: IL-2, IFN-gamma, and IL-12 gene polymorphisms and susceptibility to multiple sclerosis.
abstract: BACKGROUND
  Multiple sclerosis (MS) is a multifactorial disease. Positive genetic background could predispose individuals to this chronic disabling disease. In order to investigate the role of some proinflammatory cytokines (interleukin (IL)-2, IL-12, and interferon-gamma (IFN-gamma)) as a risk factor for MS, this study was performed.
  
  
  METHODS
  Two hundred and eleven patients with relapsing-remitting form of MS were enrolled in this study and compared with 359 healthy individuals. Using polymerase chain reaction based on sequence-specific primer method, the cytokine genes were amplified, and alleles and genotypes were detected on gel electrophoresis.
  
  
  RESULTS
  Significant increases for IFN-gamma AT (+874) genotype (54.5% vs. 37.8%, p = 0.0002) and IL-12 AA (-1188) genotype (60.8% vs. 49.7%, p = 0.014) were found in MS patients in comparison with healthy controls. A significant decrease in IFN-gamma TT (+874) genotype (17.7% vs. 27.5%, p = 0.01) and IL-12 CA (-1188) genotype (30.9% vs. 45%, p = 0.001) in MS patients was also detected. No significant differences of IL-2 G/T (-330) and IL-2 G/T (+166) in alleles and genotypes were observed between MS patients and normal subjects.
  
  
  CONCLUSIONS
  It could be suggested that the genetic variation in IL-12 A/C (-1188) and IFN-gamma A/T (+874) cytokine genes could be risk factors for MS patients.
authors: National Cell Bank of Iran, Pasteur Institute of Iran, Tehran, Iran.
authors: Mohammad Ali
authors: MA
authors: Shokrgozar
authors: Sheila
authors: S
authors: Sarial
authors: Aliakbar
authors: A
authors: Amirzargar
authors: Fazel
authors: F
authors: Shokri
authors: Nima
authors: N
authors: Rezaei
authors: Zohreh
authors: Z
authors: Arjang
authors: Jalaledin
authors: J
authors: Radfar
authors: Manijeh
authors: M
authors: Yousefi-Behzadi
authors: Mohammad
authors: M
authors: Ali Sahraian
authors: Jamshid
authors: J
authors: Lotfi
mesh_terms: D000328:Adult; D016022:Case-Control Studies; D016207:Cytokines / Q000235:genetics*; D005260:Female; D005838:Genotype; D006801:Humans; D018836:Inflammation Mediators; D007371:Interferon-gamma / Q000235:genetics; D018664:Interleukin-12 / Q000235:genetics; D007376:Interleukin-2 / Q000235:genetics; D008297:Male; D009103:Multiple Sclerosis / Q000235:genetics*; D011110:Polymorphism, Genetic; D020641:Polymorphism, Single Nucleotide; D012307:Risk Factors; D055815:Young Adult
chemical_list: D016207:Cytokines; D018836:Inflammation Mediators; D007376:Interleukin-2; D018664:Interleukin-12; D007371:Interferon-gamma