Folding DNA origami from a double-stranded source of scaffold.
Combined heat and chemical denaturation of double-stranded DNA scaffold strands in the presence of staple strands, followed by a sudden temperature drop and then stepwise dialysis to remove the chemical denaturant, leads to self-assembly of two distinct DNA-origami structures.
Högberg
Björn
B
Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115, USA. Bjorn_Hogberg@dfci.harvard.edu
Liedl
Tim
T
Shih
William M
WM
D017104:Bacteriophage M13 / Q000737:chemistry*; D004279:DNA, Viral / Q000032:analysis / Q000737:chemistry*; D005559:Formamides; D008958:Models, Molecular; D049329:Nanostructures / Q000737:chemistry*; D009690:Nucleic Acid Conformation; D009691:Nucleic Acid Denaturation; D013696:Temperature
D004279:DNA, Viral; D005559:Formamides; C031066:formamide