Chitosan derivative nanocarrier: safety evaluation,antibacterial property and ascorbyl palmitate encapsulation.
A chitosan derivative, methyl ether-terminated poly(ethylene oxide)-4-methoxycinnamolyphthaloylchitosan (PCPLC) was prepared, characterized and self-assembled into nanoparticles. Encapsulation of ascorbyl palmitate (AP) into PCPLC gave 6890.98 nm particles with encapsulation efficiency of 84% at 56% drug loading. The encapsulated AP showed significant improved stability as examined by 1H NMR spectroscopy.The obtained particles displayed no short-term cytotoxicity against the human skin melanoma A-375 cell line using the MTT assay and no short-term skin irritation on human volunteers using a single topical application as patch and photopatch tests. In addition, aqueous suspension of PCPLC nanoparticles successfully inhibited the growth of Escherichia coli ATCC 25922 and Staphylococcus aureus ATCC 25923.
Wittayasuporn
Mayura
M
Program in Biotechnology, Faculty of Science, Chulalongkorn University, Bangkok, Thailand.
Rengpipat
Sirirat
S
Palaga
Tanapat
T
Asawanonda
Pravit
P
Anumansirikul
Nattaporn
N
Wanichwecharungruang
Supasorn P
SP
D000328:Adult; D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000494:pharmacology*; D000970:Antineoplastic Agents / Q000008:administration & dosage*; D001205:Ascorbic Acid / Q000008:administration & dosage / Q000031:analogs & derivatives*; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D048271:Chitosan / Q000031:analogs & derivatives* / Q000494:pharmacology*; D004337:Drug Carriers / Q000737:chemistry / Q000494:pharmacology; D004926:Escherichia coli / Q000187:drug effects; D005260:Female; D006801:Humans; D008297:Male; D008826:Microbial Sensitivity Tests; D008875:Middle Aged; D053758:Nanoparticles / Q000737:chemistry*; D023422:Skin Irritancy Tests; D013211:Staphylococcus aureus / Q000187:drug effects; D055815:Young Adult
D000900:Anti-Bacterial Agents; D000970:Antineoplastic Agents; D004337:Drug Carriers; D048271:Chitosan; D001205:Ascorbic Acid; C031226:6-O-palmitoylascorbic acid