title: [Quantitative determination of 1-deoxynojirimycin in mulberry leaves by high-performance liquid chromatographic-tandem mass/mass spectrometry].
abstract: OBJECTIVE
  To develop a high-performance liquid chromatographic-tandem mass/mass spectrometric method to determine the concentration of 1-deoxynojirimycin (1-DNJ) in mulberry leaves.
  
  
  METHODS
  1-deoxynojirimycin was separated on an SHIMADZU HRC-NH2 column with the mobile phase consisting of acetonitrile and 0.1% formic acid. The mass spectrometric system equipped with a atmospheric pressure chemical ionization (APCI) interface was operated in Multiple Reaction Monitoring (MRM) mode.
  
  
  RESULTS
  The retention time of 1-deoxynojirimycin was 2.87 min, and the calibration curve was linear over a concentration range from 482 microg/L to 2410 microg/L, the average recovery was 95.8%. The detection limit was 53.6 microg/L.
  
  
  CONCLUSIONS
  The method is selective and sensitive for determining 1-deoxynojirimycin in mulberry leaves.
authors: Industrial Affairs Office, Guangzhou 510515, China.
authors: Kai-Jin
authors: KJ
authors: Dai
authors: Lian-Bing
authors: LB
authors: Hou
authors: Qi-Zhi
authors: QZ
authors: Luo
mesh_terms: D017485:1-Deoxynojirimycin / Q000032:analysis* / Q000302:isolation & purification; D004365:Drugs, Chinese Herbal / Q000737:chemistry; D031625:Morus / Q000737:chemistry*; D018515:Plant Leaves / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D011786:Quality Control; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012996:Solutions; D053719:Tandem Mass Spectrometry
chemical_list: D004365:Drugs, Chinese Herbal; D012996:Solutions; D017485:1-Deoxynojirimycin