[Evaluating the quality of niuhuangjiedu tablets by the systematic quantified fingerprint method].
The HPLC fingerprints of Niuhuangjiedu tablets (NHJDT) were established and assessed by systematic quantified fingerprint method. The chromatographic fingerprints (CFPs) were determined by reversed-phase HPLC, in which the CFPs containing 53 co-possessing peaks were marked by choosing baicalin peak as the referential peak. Fifteen batches of NHJDT were identified with hierarchical clustering analysis by using macro qualitative similarities and macro quantitative similarities as variables. According to the results of classification, the referential fingerprint (RFP) was synthesized from 10 batches of NHJDTs. Taking the RFP for the qualified model, the whole 15 batches of NHJDTs were evaluated by the systematic quantified fingerprint method. Among the 15 batches of NHJDT, 9 batches were completely qualified, the relative variance of leveling coefficient of 1 batch was obviously higher while the contents of other 5 batches were lower. The systematic quantified fingerprint method closely integrates the systematic fingerprints with the macro qualitative similarities and macro quantitative similarities to perform the entire qualification and quantitation analyses, which can briefly and effectively assess the quality of Chinese traditional medicine.
Sun
Guo-xiang
GX
College of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China. gxswmwys@163.com
Hu
Yue-shan
YS
Bi
Kai-shun
KS
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004338:Drug Combinations; D004353:Drug Evaluation, Preclinical; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000737:chemistry*; D011786:Quality Control; D013607:Tablets
D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; D013607:Tablets