Preparation of core-shell biodegradable microfibers for long-term drug delivery.
A coaxial electrospun technique to fabricate core-shell microfibers (MFs) for drug delivery application is described. In one-step, Paclitaxel (PTX)-loaded poly(L-lactic acid-co-epsilon-caprolactone) (75:25) (P(LLA-CL)(core/shell)) was electrospun into MFs using 2,2,2-trifluoroethanol as the solvent. The physical and chemical properties of electrospun fibers were characterized by various techniques, such as scanning electron microscopy, transmission electron microscopy, X-ray diffractometry, and Fourier-transform infrared. The fiber diameter depended on both the polymer concentration and the flow ratio of PTX to P(LLA-CL). The encapsulation efficiency and in vitro release profile were measured using high performance liquid chromatography methods. PTX released from the MFs in a short burst over 24 h followed by very slow release over the following 60 days. In addition, the cytotoxicity of PTX-loaded P(LLA-CL) MFs was evaluated using 3-[4,5-dimehyl-2-thiazolyl]-2, 5-diphenyl-2H-tetrazolium bromide assay on HeLa cell lines. These results indicate that PTX could be released from P(LLA-CL) fibers in a steady manner and effectively inhibit the activity of HeLa cells.
Huang
Hui-Hua
HH
College of Material Science and Engineering, Donghua University, Shanghai 201620, China.
He
Chuang-Long
CL
Wang
Hong-Sheng
HS
Mo
Xiu-Mei
XM
D016503:Drug Delivery Systems / Q000379:methods*; D006801:Humans; D019344:Lactic Acid; D017239:Paclitaxel / Q000008:administration & dosage*; D011091:Polyesters; D011108:Polymers / Q000138:chemical synthesis*; D014270:Trifluoroethanol
D011091:Polyesters; D011108:Polymers; C016240:polycaprolactone; D019344:Lactic Acid; C033616:poly(lactide); D014270:Trifluoroethanol; D017239:Paclitaxel