title: Plasmodium falciparum genotypes diversity in symptomatic malaria of children living in an urban and a rural setting in Burkina Faso.
abstract: BACKGROUND
  The clinical presentation of malaria, considered as the result of a complex interaction between parasite and human genetics, is described to be different between rural and urban areas. The analysis of the Plasmodium falciparum genetic diversity in children with uncomplicated malaria, living in these two different areas, may help to understand the effect of urbanization on the distribution of P. falciparum genotypes.
  
  
  METHODS
  Isolates collected from 75 and 89 children with uncomplicated malaria infection living in a rural and an urban area of Burkina Faso, respectively, were analysed by a nested PCR amplification of msp1 and msp2 genes to compare P. falciparum diversity.
  
  
  RESULTS
  The K1 allelic family was widespread in children living in the two sites, compared to other msp1 allelic families (frequency >90%). The MAD 20 allelic family of msp1 was more prevalent (p = 0.0001) in the urban (85.3%) than the rural area (63.2%). In the urban area, the 3D7 alleles of msp2 were more prevalent compared to FC27 alleles, with a high frequency for the 3D7 300bp allele (>30%). The multiplicity of infection was in the range of one to six in the urban area and of one to seven in the rural area. There was no difference in the frequency of multiple infections (p = 0.6): 96.0% (95% C.I: 91.6-100) in urban versus 93.1% (95%C.I: 87.6-98.6) in rural areas. The complexity of infection increased with age [p = 0.04 (rural area), p = 0.06 (urban area)].
  
  
  CONCLUSIONS
  Urban-rural area differences were observed in some allelic families (MAD20, FC27, 3D7), suggesting a probable impact of urbanization on genetic variability of P. falciparum. This should be taken into account in the implementation of malaria control measures.
authors: Centre National de Recherche et de Formation sur le Paludisme, 01 BP 2208 Ouagadougou 01, Burkina Faso. a.soulama.cnrfp@fasonet.bf
authors: Issiaka
authors: I
authors: Soulama
authors: Issa
authors: I
authors: Nébié
authors: Alphonse
authors: A
authors: Ouédraogo
authors: Adama
authors: A
authors: Gansane
authors: Amidou
authors: A
authors: Diarra
authors: Alfred B
authors: AB
authors: Tiono
authors: Edith C
authors: EC
authors: Bougouma
authors: Amadou T
authors: AT
authors: Konaté
authors: Gustave B
authors: GB
authors: Kabré
authors: Walter Rj
authors: WR
authors: Taylor
authors: Sodiomon B
authors: SB
authors: Sirima
mesh_terms: D000818:Animals; D000953:Antigens, Protozoan / Q000235:genetics; D002050:Burkina Faso / Q000453:epidemiology; D002675:Child, Preschool; D005260:Female; D014644:Genetic Variation; D005838:Genotype; D006801:Humans; D007223:Infant; D016778:Malaria, Falciparum / Q000453:epidemiology / Q000469:parasitology*; D008297:Male; D020066:Merozoite Surface Protein 1 / Q000235:genetics; D010963:Plasmodium falciparum / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D016133:Polymerase Chain Reaction / Q000379:methods; D015800:Protozoan Proteins / Q000235:genetics; D012424:Rural Population; D014505:Urban Population
chemical_list: D000953:Antigens, Protozoan; D020066:Merozoite Surface Protein 1; D015800:Protozoan Proteins; C065974:merozoite surface protein 2, Plasmodium