Real-time fluorescence monitoring of GSK3beta-catalyzed phosphoryation by use of a BODIPY-based Zn(II)-Dpa chemosensor.
We developed a new fluorescence assay system for GSK3beta-catalyzed kinase reaction using the BODIPY-based fluorescent chemosensor. This system exploits the selective sensing property of the chemosensor for a (i, i+4) bis-phosphorylated peptide, which allows us to conveniently detect the phosphorylation reaction with a fluorescence increase in a real-time fashion.
Sakamoto
Takashi
T
Department of Synthetic Chemistry and Biological Chemistry, Graduate School of Engineering, Kyoto University, Katsura Campus, Kyoto 615-8510, Japan.
Inoue
Masa-aki
MA
Ojida
Akio
A
Hamachi
Itaru
I
D000818:Animals; D001896:Boron Compounds / Q000494:pharmacology*; D002384:Catalysis; D002626:Chemistry, Pharmaceutical / Q000379:methods; D015195:Drug Design; D005456:Fluorescent Dyes / Q000494:pharmacology*; D038362:Glycogen Synthase Kinase 3 / Q000737:chemistry*; D006801:Humans; D007700:Kinetics; D008956:Models, Chemical; D010455:Peptides / Q000737:chemistry; D010766:Phosphorylation; D011817:Rabbits; D013997:Time Factors; D015032:Zinc / Q000737:chemistry*
C095489:4,4-difluoro-4-bora-3a,4a-diaza-s-indacene; D001896:Boron Compounds; D005456:Fluorescent Dyes; D010455:Peptides; D038362:Glycogen Synthase Kinase 3; D015032:Zinc