Fatty acid production from a highly CO2 tolerant alga, Chlorocuccum littorale, in the presence of inorganic carbon and nitrate.
Photoautotrophic fatty acid production of a highly CO(2)-tolerant green alga Chlorococcum littorale was investigated in the presence of inorganic carbon and nitrate at 295 K and a light intensity of 170 micromol-photon m(-2) s(-1). CO(2) concentration in the bubbling gas was adjusted by mixing pure gas components of CO(2) and N(2) to avoid photorespiration and beta-oxidation of fatty acids under O(2) atmosphere conditions. Fatty acid content was almost constant for the CO(2) concentrations ranging from 5% to 50% under nitrate-rich conditions corresponding to the logarithmic growth phase. After nitrate depletion, the content drastically increased with a decrease in CO(2) concentration. HCO(3)(-)/CO(2) ratio in the culture media was found to be a controlling factor for fatty acid production after the nitrate limitation phase. For a CO(2) concentration of 5%, the fatty acid content was ca. 34 wt.% at maximum, which is comparable with other land plant seed oils.
Ota
Masaki
M
Research Center of Supercritical Fluid Technology, Tohoku University, Aramaki Aza Aoba-6-6-11-403, Aoba-ku, Sendai 980-8579, Japan. masa@scf.che.tohoku.ac.jp
Kato
Yoshitaka
Y
Watanabe
Hiromoto
H
Watanabe
Masaru
M
Sato
Yoshiyuki
Y
Smith
Richard L
RL
Inomata
Hiroshi
H
D000222:Adaptation, Physiological / Q000187:drug effects*; D018533:Biomass; D002244:Carbon / Q000494:pharmacology*; D002245:Carbon Dioxide / Q000494:pharmacology*; D000460:Chlorophyta / Q000166:cytology / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism*; D005227:Fatty Acids / Q000032:analysis / Q000096:biosynthesis*; D009566:Nitrates / Q000494:pharmacology*; D013997:Time Factors
D005227:Fatty Acids; D009566:Nitrates; D002245:Carbon Dioxide; D002244:Carbon