Bifidobacterium bombi sp. nov., from the bumblebee digestive tract.
Gram-positive-staining, anaerobic, non-spore-forming, lactate- and acetate-producing bacterial strains were isolated from the digestive tracts of different bumblebee species (Bombus lucorum, Bombus pascuorum and Bombus lapidarius). All of the isolates produced fructose-6-phosphate phosphoketolase activity. A representative strain, BluCI/TPT, was characterized further. Cells of strain BluCI/TPT showed occasional bifurcation and irregular constrictions. The bacterium utilized a wide range of carbohydrates. Glucose was fermented to acetate and lactate. The DNA base composition was 47.2 mol% G+C. Complete 16S rRNA and partial hsp60 gene sequences were obtained and phylogenetic relationships were determined. Strain BluCI/TPT and related isolates were located in the actinobacterial cluster and were closely related to the genera Bifidobacterium, Scardovia, Aeriscardovia and Parascardovia. The results presented support the proposal of a novel species to accommodate strain BluCI/TPT, with the name Bifidobacterium bombi sp. nov.; the type strain is BluCI/TPT (=DSM 19703T=ATCC BAA-1567T).
Killer
J
J
Czech University of Life Sciences, Department of Microbiology, Nutrition and Dietetics, Kamýcká 129, Prague 6-Suchdol, 165 21, Czech Republic.
Kopecný
J
J
Mrázek
J
J
Rada
V
V
Benada
O
O
Koppová
I
I
Havlík
J
J
Straka
J
J
D000085:Acetates / Q000378:metabolism; D000446:Aldehyde-Lyases / Q000378:metabolism; D000693:Anaerobiosis; D000818:Animals; D015373:Bacterial Typing Techniques; D001482:Base Composition; D001516:Bees / Q000382:microbiology*; D001644:Bifidobacterium / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D050260:Carbohydrate Metabolism; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D041981:Gastrointestinal Tract / Q000382:microbiology*; D019344:Lactic Acid / Q000378:metabolism; D008855:Microscopy, Electron, Scanning; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013171:Spores, Bacterial / Q000166:cytology
D000085:Acetates; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D019344:Lactic Acid; D000446:Aldehyde-Lyases; C065556:fructose-6-phosphate phosphoketolase