Construction of phage antibody repertoires from the blood of West Nile virus-infected donors.
A method for the construction of West Nile virus immune donor antibody repertoires is described. B cells are harvested from a suitable donor and the antibody variable genes are amplified using polymerase chain reaction (PCR). The PCR fragments are cloned in a phage display vector to construct a repertoire that can be used in panning procedures to identify many unique monoclonal antibodies.
Throsby
Mark
M
Crucell Holland BV, Archimedesweg 4, Leiden, The Netherlands. mark.throsby@crucell.com
de Kruif
John
J
D000914:Antibodies, Viral / Q000276:immunology*; D000956:Antigens, Viral / Q000276:immunology; D001435:Bacteriophages / Q000276:immunology*; D001782:Blood Donors; D006801:Humans; D007143:Immunoglobulin Heavy Chains / Q000235:genetics / Q000276:immunology; D007147:Immunoglobulin Light Chains / Q000235:genetics / Q000276:immunology; D007135:Immunoglobulin Variable Region / Q000235:genetics / Q000276:immunology; D019151:Peptide Library; D015202:Protein Engineering; D012367:RNA, Viral / Q000235:genetics / Q000378:metabolism; D014901:West Nile Fever / Q000097:blood*; D014902:West Nile virus / Q000276:immunology*
D000914:Antibodies, Viral; D000956:Antigens, Viral; D007143:Immunoglobulin Heavy Chains; D007147:Immunoglobulin Light Chains; D007135:Immunoglobulin Variable Region; D019151:Peptide Library; D012367:RNA, Viral