In vitro cytotoxicity evaluation of biomedical nanoparticles and their extracts.
The present study presents a new approach for evaluating in vitro cytotoxicity of nanoparticles. The approach is based on American National Standard ISO 10993-5. Hepatoma HepG2 and fibroblast NIH3T3 cell lines were incubated with nanoparticles, and their associated extracts were derived at 70 and 121 degrees C. Nanoparticles proposed as potential biomedical imaging probes were evaluated on the basis of the detection of metabolic activities and cell-morphology changes. In general, nanoparticles incubated directly with cells showed higher cytotoxicity than their associated extracts. CdSe and core-shell CdSe@ZnS quantum dots resulted in low cell viability for both cell lines. The cytotoxicity of the quantum dots was attributed to the Cd ion and the presence of the nanoparticle itself. A statistically significant (p < 0.05) decrease in cell viability was found in higher dosage concentrations. Rare earth nanoparticles and their extracts appear to affect NIH3T3 cells only, with cell viability as low as 71.4% +/- 4.8%. Magnetic nanoparticles have no observable effects on the cell viabilities for both cell lines. In summary, we found the following: (1) both direct incubation and extracts of nanoparticles are required for complete assessment of nanoparticle cytotoxicity, (2) the rare earth oxide nanoparticles are less cytotoxic than the Cd-based quantum dots, and (3) the extent of cytotoxicity is dependent upon the cell line.
Das
Gautom Kumar
GK
School of Chemical and Biomedical Engineering, Nanyang Technological University, Singapore 637459, Singapore.
Chan
Peggy P Y
PP
Teo
Ailing
A
Loo
Joachim Say Chye
JS
Anderson
James M
JM
Tan
Timothy Thatt Yang
TT
D000818:Animals; D019187:Cadmium Compounds / Q000633:toxicity; D016923:Cell Death / Q000187:drug effects; D002457:Cell Extracts; D048430:Cell Shape / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D056945:Hep G2 Cells; D006801:Humans; D051379:Mice; D041681:NIH 3T3 Cells; D053758:Nanoparticles / Q000633:toxicity*; D018036:Selenium Compounds / Q000633:toxicity; D013057:Spectrum Analysis; D013997:Time Factors
D019187:Cadmium Compounds; D002457:Cell Extracts; D018036:Selenium Compounds; C058667:cadmium selenide