Cell-based in vivo dual imaging probes using genetically expressed tags and chemical contrast agents.
We report a method for specifically labelling the surface of cells with two kinds of chemical probes (near-infrared (NIR) fluorescent probes and magnetic resonance (MR) imaging probes) via two genetically expressed tags, and demonstrate the application for in vitro and in vivo dual imaging.
Hasegawa
Yuki
Y
Department of Applied Chemistry, Faculty of Science and Technology, Keio University, Kohoku-ku, Yokohama, 223-8522, Japan.
Honda
Aki
A
Umezawa
Keitaro
K
Niino
Yusuke
Y
Oka
Kotaro
K
Chiba
Tomohiro
T
Aiso
Sadakazu
S
Tanimoto
Akihiro
A
Citterio
Daniel
D
Suzuki
Koji
K
D000818:Animals; D001710:Biotin / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics*; D003287:Contrast Media / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D006867:Hydrolases / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics*; D008163:Luminescent Measurements; D008164:Luminescent Proteins / Q000737:chemistry / Q000235:genetics*; D008279:Magnetic Resonance Imaging; D051379:Mice; D019265:Spectroscopy, Near-Infrared
D003287:Contrast Media; D005456:Fluorescent Dyes; D008164:Luminescent Proteins; D001710:Biotin; D006867:Hydrolases