Neutralizing monoclonal antibody sandwich liquid-phase blocking enzyme-linked immunosorbent assay for detection of Foot-and-mouth disease virus type O antibodies.
Liquid-phase blocking enzyme-linked immunosorbent assay (LPBE) using the neutralizing monoclonal antibody (mAb) sandwich method (M-LPBE) for detection of Foot-and-mouth disease virus (FMDV) type O antibodies was developed. Two neutralizing mAbs, 72C1 and 65H6, were raised against the FMDV O/JPN/2000 strain, and used as trapping and peroxidase-labeled detecting antibodies, respectively. Sera from animals experimentally infected with FMDV showed specific positive results by M-LPBE, which were correlated with the results of the virus neutralization test (VNT). When 303 negative bovine and 302 negative swine sera were tested, the specificity of M-LPBE was 100% and 99.7%, respectively. In addition, nine samples that had been collected in 2000 in Japan and regarded as evidently false positives by LPBE (supplied by the World Reference Laboratory for Foot-and-Mouth Disease) were uniformly negative by M-LPBE, just like VNT. Therefore, M-LPBE seems to have sufficient specificity for FMDV type O antibody screening and diagnosis.
Morioka
Kazuki
K
Exotic Diseases Research Station, National Institute of Animal Health, Josuihoncho 6-20-1 Kodaira Tokyo 187-0022, Japan. morioka@affrc.go.jp
Fukai
Katsuhiko
K
Yoshida
Kazuo
K
Yamazoe
Reiko
R
Onozato
Hiroyuki
H
Ohashi
Seiichi
S
Tsuda
Tomoyuki
T
Sakamoto
Kenichi
K
D000818:Animals; D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral / Q000097:blood*; D002417:Cattle; D002418:Cattle Diseases / Q000097:blood; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000662:veterinary*; D005536:Foot-and-Mouth Disease / Q000097:blood* / Q000453:epidemiology / Q000821:virology; D029544:Foot-and-Mouth Disease Virus / Q000145:classification / Q000276:immunology*; D007564:Japan / Q000453:epidemiology; D013552:Swine; D013553:Swine Diseases / Q000097:blood
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral