Risk of contamination of germplasm during cryopreservation and cryobanking in IVF units.
Cryopreservation of sperm, embryos and, more recently, oocytes plays an important and increasing role in assisted reproduction, due to improvements of old, and introduction of new technologies. In parallel, concerns are increasing about the technical and biological safety of these procedures. However, published data regarding the confirmed or theoretical hazards of these procedures are sparse and sometimes contradictory. The purpose of this review will summarize data and opinions about one of the most disputed risks, the potential hazard of contamination and disease transmission through cryopreservation. Special attention is concentrated on the weak points of the technology including open vitrification systems, sterilization of liquid nitrogen and safety of commonly used storage tanks including straws and cryovials. Suggestions are also made for practical measures to avoid these dangers while preserving the benefits and perspectives of new cryopreservation technologies.
Bielanski
A
A
Canadian Food Inspection Agency, Animal Diseases Research Institute, Germplasm Centre of Expertise, Ottawa/Nepean, ON, Canada K2H 8P9. bielanskia@inspection.gc.ca
Vajta
G
G
D018070:Biological Specimen Banks; D015925:Cryopreservation / Q000295:instrumentation / Q000379:methods*; D003451:Cryoprotective Agents; D046149:Embryo Culture Techniques; D004622:Embryo, Mammalian / Q000382:microbiology / Q000821:virology; D004866:Equipment Contamination; D005260:Female; D005307:Fertilization in Vitro; D006801:Humans; D008297:Male; D009584:Nitrogen / Q000737:chemistry; D009865:Oocytes / Q000382:microbiology / Q000821:virology; D018570:Risk Assessment; D012661:Semen / Q000382:microbiology / Q000821:virology; D013048:Specimen Handling; D013094:Spermatozoa / Q000382:microbiology / Q000821:virology
D003451:Cryoprotective Agents; D009584:Nitrogen