Expression of IL-1alpha, IL-6, TGF-beta, FasL and ZNF265 during sertoli cell infection by ureaplasma urealyticum.
To investigate immunoregulatory mechanisms of Sertoli cells in the testis in vitro and in vivo, we utilized our well-characterized Ureaplasma Urealyticum (UU)-induced model. We investigated the expressions of IL-1alpha, IL-6, TGF-beta, FasL and ZNF265 at the first, second and third weeks post-infection. During recovery from inflammation and with the help of negative regulators TGF-beta and FasL, the high levels of IL-1alpha and IL-6 expressions were observed in the early stages of the infection, and decreased gradually in the later weeks both in vitro and in vivo. The trend of varied expression of ZNF265 was similar to those of TGF-beta and FasL in vitro and in vivo for Sertoli cells infected with UU.
Li
Rongping
R
Shanghai Jiao Tong University School of Medicine, Department of Immunology, Shanghai Institute of Immunology, Shanghai 200025, China.
Xi
Yebin
Y
Liu
Xiuzhi
X
Chen
Guangjie
G
Wang
Baoguo
B
Jiang
Lihua
L
Li
Weiyi
W
D000818:Animals; D002478:Cells, Cultured; D004797:Enzyme-Linked Immunosorbent Assay; D053222:Fas Ligand Protein / Q000235:genetics* / Q000378:metabolism; D005434:Flow Cytometry; D015870:Gene Expression; D054884:Host-Pathogen Interactions; D007150:Immunohistochemistry; D053582:Interleukin-1alpha / Q000235:genetics* / Q000378:metabolism; D015850:Interleukin-6 / Q000235:genetics* / Q000378:metabolism; D008297:Male; D016601:RNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012708:Sertoli Cells / Q000166:cytology / Q000378:metabolism* / Q000382:microbiology; D013737:Testis / Q000378:metabolism / Q000382:microbiology; D013997:Time Factors; D016212:Transforming Growth Factor beta / Q000235:genetics* / Q000378:metabolism; D016869:Ureaplasma Infections / Q000235:genetics / Q000378:metabolism / Q000382:microbiology; D016990:Ureaplasma urealyticum / Q000502:physiology
D053222:Fas Ligand Protein; D053582:Interleukin-1alpha; D015850:Interleukin-6; D016601:RNA-Binding Proteins; D016212:Transforming Growth Factor beta; C109244:Zranb2 protein, rat