title: Iron-limited condition modulates biofilm formation and interaction with human epithelial cells of enteroaggregative Escherichia coli (EAEC).
abstract: OBJECTIVE
  The aim of this study was to investigate the influence of low iron availability on biofilm formation and adherence to HEp-2 cells of enteroaggregative Escherichia coli (EAEC) strains isolated from diarrhoea cases.
  
  
  RESULTS
  The ability of EAEC to form biofilm on a plastic surface was evaluated quantitatively and qualitatively after 3 and 18 h of incubation of strains with or without the iron chelator 2,2-dipyridyl. When submitted to low iron conditions, prototype EAEC 042 strain showed a decrease in biofilm formation. Conversely, an increase in biofilm formation was observed for the clinical EAEC strains cultured in restricted iron condition. Moreover, the reduction of iron concentration inhibited the aggregative adherence to HEp-2 cells of all EAEC strains tested. However, all effects promoted by iron chelation were suppressed by thiourea.
  
  
  CONCLUSIONS
  Low iron availability may modulate biofilm formation and adhesive properties of EAEC strains to HEp-2 cells.
  
  
  CONCLUSIONS
  The data obtained in this study provide useful insights on the influence of low iron conditions possibly associated with redox stress on the pathogenesis of EAEC strains.
authors: Departamento de Microbiologia, Imunologia e Parasitologia, Faculdade de Ciências Médicas, Universidade do Estado do Rio de Janeiro, Rio de Janeiro, RJ, Brazil.
authors: J R
authors: JR
authors: Alves
authors: A C M
authors: AC
authors: Pereira
authors: M C
authors: MC
authors: Souza
authors: S B
authors: SB
authors: Costa
authors: A S
authors: AS
authors: Pinto
authors: A L
authors: AL
authors: Mattos-Guaraldi
authors: R
authors: R
authors: Hirata-Júnior
authors: A C P
authors: AC
authors: Rosa
authors: L M B O
authors: LM
authors: Asad
mesh_terms: D015082:2,2'-Dipyridyl / Q000494:pharmacology; D001422:Bacterial Adhesion / Q000187:drug effects / Q000502:physiology*; D018441:Biofilms / Q000187:drug effects / Q000254:growth & development*; D002460:Cell Line; D002614:Chelating Agents / Q000494:pharmacology; D004847:Epithelial Cells / Q000382:microbiology*; D004926:Escherichia coli / Q000502:physiology*; D006801:Humans; D007501:Iron / Q000037:antagonists & inhibitors / Q000378:metabolism*; D018384:Oxidative Stress / Q000187:drug effects
chemical_list: D002614:Chelating Agents; D015082:2,2'-Dipyridyl; D007501:Iron