title: Suppression of dendritic cells by HMGB1 is associated with lymph node metastasis of human colon cancer.
abstract: High mobility groupbox-1 (HMGB1) is a multifunctional cytokine secreted by cancer cells, which accelerates cell growth, invasion and angiogenesis in cancer, and induces apoptosis in macrophages. Thioglycolate-stimulated mouse peritoneal macrophages were induced to differentiate into dendritic cells by co-treatment with IL-4 and GM-CSF. The number of mouse peritoneal macrophage-derived dendritic cells (PMDDCs) showed a dose-dependent decrease in hrHMGB1 treatment. HMGB1-treated PMDDCs showed obvious apoptosis and increased the level of phosphorylated JNK. Intraperitoneal administration of HMGB1 decreased CD205-positive splenic dendritic cells in C57BL mice. To confirm the HMGB1-induced inhibitory effect on dendritic cells, 16 cases of human colon cancer invaded into the subserosal layer were examined. The 8 nodal metastasis-positive cases showed higher nodal HMGB1 concentrations (74 +/- 23 vs. 41 +/- 15 microg/ml, p = 0.0116) in lymph node tissues and lower CD205-positive nodal dendritic cell numbers (86 +/- 22 vs. 137 +/- 43/mm(2), p = 0.0224) than those in the 8 metastasis-negative cases. Primary tumor tissues of metastasis-positive cases showed higher tumor HMGB1 levels (116 +/- 33 vs. 37 +/- 18 microg/ml, p = 0.0007) and lower CD205-positive intratumoral dendritic cell numbers (21 +/- 13 vs. 62 +/- 23 /mm(2), p = 0.0068) than those in metastasis-negative cases. These findings suggest that HMGB1 produced by colon cancer cells suppressed nodal dendritic cells to disturb host anti-cancer immunity.
authors: Department of Molecular Pathology, Nara Medical University, Kashihara, Japan.
authors: Akiko
authors: A
authors: Kusume
authors: Tomonori
authors: T
authors: Sasahira
authors: Yi
authors: Y
authors: Luo
authors: Mamoru
authors: M
authors: Isobe
authors: Naoya
authors: N
authors: Nakagawa
authors: Naokuni
authors: N
authors: Tatsumoto
authors: Kiyomu
authors: K
authors: Fujii
authors: Hitoshi
authors: H
authors: Ohmori
authors: Hiroki
authors: H
authors: Kuniyasu
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D002452:Cell Count; D002454:Cell Differentiation / Q000187:drug effects; D003110:Colonic Neoplasms / Q000276:immunology* / Q000378:metabolism / Q000473:pathology; D016207:Cytokines / Q000276:immunology / Q000378:metabolism / Q000494:pharmacology; D003713:Dendritic Cells / Q000187:drug effects* / Q000276:immunology* / Q000378:metabolism / Q000473:pathology; D024243:HMGB1 Protein / Q000276:immunology* / Q000378:metabolism / Q000494:pharmacology; D006801:Humans; D007108:Immune Tolerance / Q000187:drug effects; D066298:In Vitro Techniques; D008207:Lymphatic Metastasis / Q000276:immunology* / Q000473:pathology / Q000503:physiopathology; D017737:Macrophages, Peritoneal / Q000187:drug effects / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D011994:Recombinant Proteins / Q000494:pharmacology
chemical_list: D016207:Cytokines; D024243:HMGB1 Protein; D011994:Recombinant Proteins