[Complete blood count reference values of donated cord blood from Korean neonates].
BACKGROUND
In the public cord blood (CB) banks, only safe CB units with adequate cell doses are processed and stored. Complete blood count (CBC) of CB is crucial for estimating total nucleated cells (TNC) and screening suitable CB units without hematologic abnormalities. We analyzed CBC parameters of the donated CB from healthy Korean neonates to establish CBC reference values.


METHODS
A total of 2,129 Korean CB units, donated and processed during the period from August 2007 to December 2007, were enrolled. We measured hemoglobin (Hb), white blood cell (WBC) count, differential count of WBC, platelets and nucleated red blood cell (nRBC) count by XE-2100 automated hematology analyzer (Sysmex, Japan), and estimated reference value of each parameter by using parametric (Mean+/-2SD) and/or non-parametric methods (2.5-97.5 percentile). And also, we compared the result of each parameter in relation to sex of neonates and delivery method.


RESULTS
Because the differences of CBC values among different subgroups were not remarkable, we established the reference intervals as follows without subgroup division: Hb, 9.0-14.4 g/dL; WBC count, 5.6-18.5 x 10(3)/microL; differential count of WBC (neutrophils, 40.8-72.4%; lymphocytes, 17.2-46.7%; monocytes, 4.9-12.8%; eosinophils, 0.7-7.0%; basophils, 0.0-1.6%); platelet, 130-287 x 10(3)/microL; nRBCs, 0.0-13.1/100 WBC.


CONCLUSIONS
We established cord blood CBC reference values of healthy Korean neonates using a large-scale CB units. The established CBC reference values from our study will be useful as basic data for CBC interpretation and assessment of transplant suitability of donated CB.
Lee
Hye Ryun
HR
Department of Laboratory Medicine, Seoul National University College of Medicine, Dongjak-Gu, Seoul, Korea.
Shin
Sue
S
Yoon
Jong Hyun
JH
Kim
Byoung Jae
BJ
Hwang
Kyu Ri
KR
Kim
Jin Ju
JJ
Roh
Eun Youn
EY
D001772:Blood Cell Count / Q000592:standards*; D005260:Female; D005312:Fetal Blood / Q000166:cytology*; D006801:Humans; D007231:Infant, Newborn; D007723:Korea; D008297:Male; D012016:Reference Values