Analysis of STAT5A/AvaI gene polymorphism in four Italian cattle breeds.
The STAT5A/AvaI polymorphism was investigated with PCR-RFLP in a sample of 339 cattle belonging to four breeds: Italian Friesian, Jersey, Italian Brown, and Podolica reared in south Italy. All three possible genotypes for the C/T polymorphism were identified. In these breeds, PCR-RFLP showed the predominance of the TT genotype in Italian Brown and Jersey cows; in Podolica and Italian Friesian CT is the most frequent genotype. The frequency of the T allele ranged from 0.55 to 0.81 in the analyzed populations. The distribution of genotypic and allelic frequencies at this locus was significantly different among the four populations based on a chi2 test (P < 0.001), suggesting that the molecular characteristics of the STAT5A gene could be significantly affected by the breed selection. Gene heterozygosity, gene homozygosity, effective allele number, fixation index, and polymorphism information content (PIC) were calculated. The observed heterozygosity, as well as the Ne and PIC values, indicates high genetic variability in the Podolica breed. Podolica could be considered an interesting reservoir of genetic diversity for a species under high selective pressure elsewhere.
Dario
C
C
Department of Health and Welfare of Animals, University of Studies of Bari, Prov le per Casamassima Km 3, 70010 Valenzano, Italy. c.dario@veterinaria.uniba.it
Dario
M
M
Ciotola
F
F
Peretti
V
V
Carnicella
D
D
Selvaggi
M
M
D000818:Animals; D001947:Breeding; D002417:Cattle / Q000145:classification* / Q000235:genetics*; D005787:Gene Frequency; D014644:Genetic Variation; D005838:Genotype; D007558:Italy; D011110:Polymorphism, Genetic; D050799:STAT5 Transcription Factor / Q000235:genetics*
D050799:STAT5 Transcription Factor