title: In idiopathic calcium oxalate stone-formers, unattached stones show evidence of having originated as attached stones on Randall's plaque.
abstract: OBJECTIVE
  To analyse the structure and composition of unattached stones in idiopathic calcium oxalate (CaOx) stone-formers (ICSF) and compare them to attached stones from the same cohort, to investigate whether there is more than one pathogenic mechanism for stone formation in ICSF.
  
  
  METHODS
  ICSF undergoing percutaneous nephrolithotomy or ureteroscopy for the treatment of nephrolithiasis gave consent to participate in this study. All accessible renal papillae were endoscopically imaged using a digital endoscope. All stones were removed and determined by the operating surgeon to be attached or unattached to the underlying papilla. Micro-computed tomography (micro-CT), which provides three-dimensional analysis of entire stones, was used to compare the structure and composition of attached and unattached stones.
  
  
  RESULTS
  Of 115 stones collected from nine patients (12 renal units), only 25 stones were found not to be attached to renal papillae. Of these 25 stones, four were lost and 12 showed definite morphological evidence of having been attached to tissue, probably having been displaced from papillae during access. For the remaining nine stones, micro-CT analysis showed at least one internal region of calcium phosphate within each of these unattached CaOx stones, i.e. the internal structure of the unattached stones is consistent with their having originated attached to Randall's plaque, and then having become detached but retained in the kidney, with new layers of CaOx eventually covering the original attachment site. CONCLUSIONS; Micro-CT analysis supports the hypothesis that in ICSF, both attached and unattached stones occur as a result of a common pathogenic mechanism, i.e. in this type of stone former, CaOx stones, even those not showing morphology that betrays attachment, all originate attached to interstitial plaque on the renal papilla.
authors: Department of Urologic Surgery, Vanderbilt University School of Medicine, Nashville, TN 37232,, USA. nicole.miller@vanderbilt.edu
authors: Nicole L
authors: NL
authors: Miller
authors: James C
authors: JC
authors: Williams
authors: Andrew P
authors: AP
authors: Evan
authors: Sharon B
authors: SB
authors: Bledsoe
authors: Fredric L
authors: FL
authors: Coe
authors: Elaine M
authors: EM
authors: Worcester
authors: Larry C
authors: LC
authors: Munch
authors: Shelly E
authors: SE
authors: Handa
authors: James E
authors: JE
authors: Lingeman
mesh_terms: D000328:Adult; D000368:Aged; D002129:Calcium Oxalate / Q000032:analysis*; D015331:Cohort Studies; D005260:Female; D006801:Humans; D007669:Kidney Calculi / Q000737:chemistry / Q000209:etiology* / Q000473:pathology / Q000601:surgery; D007679:Kidney Medulla; D008297:Male; D008875:Middle Aged; D009403:Nephrostomy, Percutaneous / Q000379:methods; D012008:Recurrence; D014057:Tomography, X-Ray Computed
chemical_list: D002129:Calcium Oxalate