[Effect of t10, c12-conjugated linoleic acid on fatty acid metabolism in C2C12 myotubes].
OBJECTIVE
To study the effect of t10, c12-conjugated linoleic acid (t10, c12-CLA) on fatty acid metabolism in myotubes under the high free fatty acid condition.


METHODS
We used palmitate to simulate a high free fatty acid condition for C2C12 myotubes in vitro and administered t10, c12-CLA across the groups. The expression and translocation of FAT/CD36 were determined by fluorimetric immunifaction using laser scanning confocal microscope. A series of enzymes related to fatty acid metabolism, together with AMP-activated protein kinase (AMPK), were determined by western blot.


RESULTS
t10, c12-CLA dose-dependently increased the translocation of FAT/CD36 to cellular membranes, and increased the expression of p-ACC and CPT-1 in myotubes under the high free fatty acid condition. Moreover, t10, c12-CLA activated the AMPK in myotubes.


CONCLUSIONS
CLA can increased the fatty acid oxidation in myotubes via activating AMPK.
Qin
Hong
H
Department of Nutrition and Food Hygiene, School of Public Health, Harbin Medical University, Harbin 150086, China. qinhong_1104@yahoo.com.cn
Li
Ying
Y
Shi
Lin
L
Sun
Changhao
C
D055372:AMP-Activated Protein Kinases / Q000378:metabolism*; D000818:Animals; D018955:CD36 Antigens / Q000378:metabolism; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D005227:Fatty Acids / Q000378:metabolism*; D044243:Linoleic Acids, Conjugated / Q000494:pharmacology*; D051379:Mice; D018485:Muscle Fibers, Skeletal / Q000166:cytology / Q000378:metabolism*
D018955:CD36 Antigens; D005227:Fatty Acids; D044243:Linoleic Acids, Conjugated; C496197:trans-10,cis-12-conjugated linoleic acid; D055372:AMP-Activated Protein Kinases