title: [Initial RAPD analysis of Isatis indigotica somatic clones' resistance to root rot disease].
abstract: OBJECTIVE
  To make initial identification on Isatis Indigotica Somatic Clones' resistant mutant.
  
  
  METHODS
  The resistant plants were selected under the treatment of root-rot disease crude toxin. The DNA of the same clones and their parents were analysed by RAPD technology.
  
  
  RESULTS
  We got initial resistance of the plants and selected 12 primers of which most were identical but the less were different. The genetic background of the somatic clones was identical but the individuals were mutated on the DNA level.
  
  
  CONCLUSIONS
  It provides experimental foundation for further study on separating resistant mutant and resistant gene.
authors: Department of Biology Science, Tangshan Teachers College, Tangshan 063000, China. chenguiping968@sohu.com
authors: Gui-Ping
authors: GP
authors: Chen
authors: Shao-Ying
authors: SY
authors: Ke
authors: Yu-Qin
authors: YQ
authors: Chen
mesh_terms: D002999:Clone Cells; D017931:DNA Primers; D018744:DNA, Plant / Q000235:genetics / Q000302:isolation & purification; D031219:Isatis / Q000235:genetics* / Q000254:growth & development; D010935:Plant Diseases / Q000235:genetics* / Q000382:microbiology; D010946:Plants, Medicinal / Q000235:genetics* / Q000254:growth & development; D019105:Random Amplified Polymorphic DNA Technique; D012639:Seeds / Q000254:growth & development; D046509:Tissue Culture Techniques
chemical_list: D017931:DNA Primers; D018744:DNA, Plant