title: A genome-wide screen for genes affecting eisosomes reveals Nce102 function in sphingolipid signaling.
abstract: The protein and lipid composition of eukaryotic plasma membranes is highly dynamic and regulated according to need. The sphingolipid-responsive Pkh kinases are candidates for mediating parts of this regulation, as they affect a diverse set of plasma membrane functions, such as cortical actin patch organization, efficient endocytosis, and eisosome assembly. Eisosomes are large protein complexes underlying the plasma membrane and help to sort a group of membrane proteins into distinct domains. In this study, we identify Nce102 in a genome-wide screen for genes involved in eisosome organization and Pkh kinase signaling. Nce102 accumulates in membrane domains at eisosomes where Pkh kinases also localize. The relative abundance of Nce102 in these domains compared with the rest of the plasma membrane is dynamically regulated by sphingolipids. Furthermore, Nce102 inhibits Pkh kinase signaling and is required for plasma membrane organization. Therefore, Nce102 might act as a sensor of sphingolipids that regulates plasma membrane function.
authors: Organelle Architecture and Dynamics, Max Planck Institute of Biochemistry, 82152 Martinsried, Germany.
authors: Florian
authors: F
authors: Fröhlich
authors: Karen
authors: K
authors: Moreira
authors: Pablo S
authors: PS
authors: Aguilar
authors: Nina C
authors: NC
authors: Hubner
authors: Matthias
authors: M
authors: Mann
authors: Peter
authors: P
authors: Walter
authors: Tobias C
authors: TC
authors: Walther
mesh_terms: D002462:Cell Membrane / Q000737:chemistry / Q000378:metabolism; D004843:Epistasis, Genetic; D020869:Gene Expression Profiling; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism; D046912:Multiprotein Complexes / Q000737:chemistry / Q000378:metabolism; D010750:Phosphoproteins / Q000235:genetics / Q000378:metabolism; D006180:Proton-Translocating ATPases / Q000235:genetics / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000235:genetics / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction / Q000502:physiology*; D013107:Sphingolipids / Q000378:metabolism*
chemical_list: D008565:Membrane Proteins; D046912:Multiprotein Complexes; C525535:NCE102 protein, S cerevisiae; C487327:PIL1 protein, S cerevisiae; D010750:Phosphoproteins; D011993:Recombinant Fusion Proteins; C502213:SUR7 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D013107:Sphingolipids; C092477:PMA1 protein, S cerevisiae; D006180:Proton-Translocating ATPases