The difference in oxidative stress of the blood between using 5% glucose water and distilled water as the irrigant for BPH patients undergoing transurethral resection of the prostate.
BACKGROUND
To evaluate the difference in oxidative stress in the blood between using 5% glucose water and using distilled water as the irrigant for BPH patients receiving transurethral resection of the prostate (TURP), we conducted this prospective study, on a total of 38 patients with symptomatic BPH.


METHODS
All the patients were randomly divided into 2 groups. Distilled water in group A and 5% glucose water in group B were used as the irrigant. The oxidative stress and antioxidant capacity were evaluated by measuring malondialdehyde (MDA), mitochondrial DNA (mtDNA) copy number and total capacity of antioxidants (TOA) in the blood. The data were correlated with serum creatinine and sodium, which were measured before and immediately after TURP in all patients.


RESULTS
Serum creatinine increased from 0.86 +/- 0.17 to 1.02 +/- 0.20 mg/dL while serum sodium decreased from 139.7 +/- 2.3 to 136.4 +/- 4.0 mEq/L immediately after surgery in patients of group A (p < 0.05). MDA was increased and TOA decreased in all the patients immediately after TURP. Patients in group A had significantly higher MDA, lower mtDNA copy number, higher degree of oxidative mtDNA damage and lower TOA than those in group B immediately after TURP.


CONCLUSIONS
In conclusion, we observed an increase of MDA and decrease of TOA in blood in all the patients immediately after TURP, which might induce renal damage and decrease serum sodium. Moreover, the oxidative stress levels in blood of patients in group A were significantly higher than those in group B immediately after TURP.
Chen
Shiou-Sheng
SS
Department of Biochemistry and Molecular Biology, Department of Urology, School of Medicine, National Yang-Ming University, No. 155, Li-Nong St., Sec. 2, Taipei 112, Taiwan.
Wu
Shi-Bei
SB
Wei
Yau-Huei
YH
D005947:Glucose / Q000008:administration & dosage*; D006801:Humans; D008297:Male; D018384:Oxidative Stress; D011446:Prospective Studies; D011470:Prostatic Hyperplasia / Q000097:blood* / Q000378:metabolism / Q000601:surgery*; D007507:Therapeutic Irrigation; D020728:Transurethral Resection of Prostate; D014867:Water / Q000008:administration & dosage*
D014867:Water; D005947:Glucose