Lanostanes from Phellinus igniarius and their iNOS inhibitory activities.
Four new lanostanol-type triterpenoids, igniarens A - D ( 1- 4), were isolated from the fruit body of Phellinus igniarius together with two known triterpenoids, and two known ergostanes. These four new compounds were identified by spectroscopic analysis as 22 R-hydroxy-24-methylene-29-norlanost-7, 9(11)-dien-3-one (1), 3alpha,22 R-dihydroxy-24-methylene-29-norlanost-7, 9(11)-diene (2), 3alpha,22 R-dihydroxy-24-methylene-29-norlanost-8-ene ( 3), and 3alpha,22 R-dihydroxy-24-methylenelanost-8-ene ( 4). Their effects on NO production in lipopolysaccharide (LPS)-activated macrophages were assessed. Compounds 1- 8 inhibited NO production in activated RAW 264.7 cells to various degrees. The most potent compound 5alpha,8alpha-epidioxy-22 E-ergosta-6,22-dien-3beta-ol ( 7) significantly inhibited LPS-induced NO production in a concentration-dependent manner without affecting the cellular viability, with an IC (50) of 37.57 +/- 1.38 microM.
Wang
Guei-Jane
GJ
National Research Institute of Chinese Medicine, Taipei 112, Taiwan.
Tsai
Tung-Hu
TH
Chang
Tun-Tschu
TT
Chou
Cheng-Jen
CJ
Lin
Lie-Chwen
LC
D000818:Animals; D000893:Anti-Inflammatory Agents / Q000302:isolation & purification* / Q000494:pharmacology; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D048690:Fruiting Bodies, Fungal; D005658:Fungi / Q000737:chemistry*; D020128:Inhibitory Concentration 50; D007810:Lanosterol / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D008070:Lipopolysaccharides; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism; D051379:Mice; D015394:Molecular Structure; D009569:Nitric Oxide / Q000037:antagonists & inhibitors / Q000096:biosynthesis; D052247:Nitric Oxide Synthase Type II / Q000037:antagonists & inhibitors*; D014315:Triterpenes / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology
C548765:22R-hydroxy-24-methylene-29-norlanost-7, 9(11)-dien-3-one; D000893:Anti-Inflammatory Agents; D008070:Lipopolysaccharides; D014315:Triterpenes; D007810:Lanosterol; D009569:Nitric Oxide; D052247:Nitric Oxide Synthase Type II