A novel method for the preparation of retinoic acid-loaded nanoparticles.
The goal of present work was to investigate the use of bioerodible polymeric nanoparticles as carriers of retinoic acid (RA), which is known to induce differentiation of several cell lines into neurons. A novel method, named "Colloidal-Coating", has been developed for the preparation of nanoparticles based on a copolymer of maleic anhydride and butyl vinyl ether (VAM41) loaded with RA. Nanoparticles with an average diameter size of 70 nm and good morphology were prepared. The activity of the encapsulated RA was evaluated on SK-N-SH human neuroblastoma cells, which are known to undergo inhibition of proliferation and neuronal differentiation upon treatment with RA. The activity of RA was not affected by the encapsulation and purification processes.
Errico
Cesare
C
Laboratory of Bioactive Polymeric Materials for Biomedical & Environmental Applications (BIOlab), UdR-INSTM, Department of Chemistry and Industrial Chemistry, University of Pisa, Via Vecchia Livornese 1291, 56122 S. Piero a Grado, Pisa, Italy.
Gazzarri
Matteo
M
Laboratory of Bioactive Polymeric Materials for Biomedical & Environmental Applications (BIOlab), UdR-INSTM, Department of Chemistry and Industrial Chemistry, University of Pisa, Via Vecchia Livornese 1291, 56122 S. Piero a Grado, Pisa, Italy.
Chiellini
Federica
F
Laboratory of Bioactive Polymeric Materials for Biomedical & Environmental Applications (BIOlab), UdR-INSTM, Department of Chemistry and Industrial Chemistry, University of Pisa, Via Vecchia Livornese 1291, 56122 S. Piero a Grado, Pisa, Italy.
D002454:Cell Differentiation / Q000187:drug effects*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D003102:Colloids / Q000737:chemistry; D004987:Ethers / Q000737:chemistry; D006801:Humans; D008299:Maleic Anhydrides / Q000737:chemistry; D053758:Nanoparticles / Q000737:chemistry*; D009447:Neuroblastoma; D023822:Tissue Engineering / Q000379:methods; D014212:Tretinoin / Q000737:chemistry*; D014753:Vinyl Compounds / Q000737:chemistry
D003102:Colloids; D004987:Ethers; D008299:Maleic Anhydrides; D014753:Vinyl Compounds; C521980:butyl vinyl ether; D014212:Tretinoin