[Effects of glucocorticoid on calcium-activated chloride channel expression in nasal mucosa in allergic rhinitis rats].
OBJECTIVE
To investigate the expression of CLCA3 and Muc5ac in nasal mucosa in allergic rhinitis rats and the effects of glucocorticoid on its expression.


METHODS
Thirty SD rats were randomly divided into allergic rhinitis group, dexamethasone group and control group. Expression of CLCA3 mRNA and Muc5ac protein in nasal mucosa were detected by RT-PCR and immunohistochemical assay, respectively.


RESULTS
CLCA3 mRNA and Muc5ac protein in allergic rhinitis group were significantly higher than those in control group (t = 8.565, 5.317, P < 0.01, respectively). The increased expression of CLCA3 mRNA in allergic rhinitis group was well correlated with the expression of Muc5ac protein and the correlation coefficient was 0.813 (P < 0.05). After treatment with dexamethasone, the expression of CLCA3 mRNA and Muc5ac protein was notably lower than that in allergic rhinitis group (t = 3.102, 2.226, P < 0.05, respectively).


CONCLUSIONS
The stronger gene expression of CLCA3 exists, complicated with mucus overproduction in the nasal mucosa of allergic rhinitis rats. CLCA3 expression may play a pivotal role in mucus overproduction in allergic rhinitis. Dexamethasone substantially downregulates the expression of CLCA3 mRNA and Muc5ac protein.
Wu
Wen-li
WL
Department Otorhinolaryngology Head and Neck Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
Zhen
Hong-tao
HT
Gao
Min
M
D000818:Animals; D002120:Calcium Channel Agonists / Q000378:metabolism*; D018118:Chloride Channels / Q000378:metabolism*; D003907:Dexamethasone / Q000494:pharmacology; D005260:Female; D005938:Glucocorticoids / Q000494:pharmacology*; D055271:Mucin 5AC / Q000378:metabolism*; D009297:Nasal Mucosa / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D012221:Rhinitis, Allergic, Perennial / Q000378:metabolism*
D002120:Calcium Channel Agonists; D018118:Chloride Channels; D005938:Glucocorticoids; C524680:Muc5ac protein, rat; D055271:Mucin 5AC; D003907:Dexamethasone