Investigating the immunologic effects of CoCr nanoparticles.
The increase in metal-on-metal hip arthroplasties has led to concern regarding the effect of raised serum and tissue metal ion levels. Our aim was to determine changes in the integrity and function of cells of the immune system after exposure to CoCr nanoparticles in specific cell culture experiments. Nanometer-sized particles of CoCr were made from a manufacturer's forged CoCr used for metal-on-metal articulations. Primary, murine dendritic cells and T and B lymphocytes then were exposed to these CoCr particles under cell culture conditions and then assayed for viability and proliferation/activation. CoCr nanoparticles did not directly activate dendritic cells or regulate B cells. Although nanoparticles were not directly toxic to resting T cells, Signals 1- and 2-dependent T cell proliferation were reduced. This may explain the observed reduction in CD8+ T cells observed in patients with metal-on-metal implants.
Ogunwale
Bamikole
B
Department of Clinical Radiology, Guys and St Thomas' Hospital, London, SE1 7EH, UK. kolekole@doctors.org.uk
Schmidt-Ott
Andreas
A
Meek
R M Dominic
RM
Brewer
James M
JM
D000818:Animals; D001402:B-Lymphocytes / Q000166:cytology / Q000276:immunology*; D015496:CD4-Positive T-Lymphocytes / Q000166:cytology / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000166:cytology / Q000276:immunology; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D002858:Chromium Alloys / Q000032:analysis*; D004195:Disease Models, Animal; D005260:Female; D006622:Hip Prosthesis / Q000009:adverse effects; D008213:Lymphocyte Activation / Q000187:drug effects / Q000276:immunology*; D008670:Metals; D051379:Mice; D008807:Mice, Inbred BALB C; D046529:Microscopy, Electron, Transmission; D053758:Nanoparticles / Q000008:administration & dosage*; D011474:Prosthesis Design; D011475:Prosthesis Failure; D011897:Random Allocation; D012680:Sensitivity and Specificity
D002858:Chromium Alloys; D008670:Metals