The 3'-untranslated region of rice glutelin GluB-1 affects accumulation of heterologous protein in transgenic rice.
We compared the effect of the rice storage protein glutelin B-1 (GluB-1) terminator with the nopaline synthase (Nos) terminator on the accumulation of the modified house dust mite allergen mDer f 2 driven by the maize ubiquitin promoter in transgenic rice. Accumulation of mDer f 2 in transgenic seed and leaf using the GluB-1 terminator was greater than when using the Nos terminator construct. The mDer f 2 mRNA containing the GluB-1 3'UTR was processed and polyadenylated at the same sites as the native GluB-1 mRNA in the seeds but diverged in leaves of the transgenic plants. In contrast, the poly(A) sites of mDer f 2 containing Nos 3'UTR were more divergent in both seed and leaf. These results suggest that GluB-1 3'UTR functions as a faithful terminator and that termination at the specific sites may play an important role in mRNA stability and/or translatability, resulting in higher levels of protein accumulation.
Yang
Lijun
L
Transgenic Crop Research and Development Center, National Institute of Agrobiological Sciences, Kannondai 2-1-2, Tsukuba, Ibaraki, 305-8602, Japan.
Wakasa
Yuhya
Y
Kawakatsu
Taiji
T
Takaiwa
Fumio
F
D020413:3' Untranslated Regions; D000485:Allergens / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D001483:Base Sequence; D005983:Glutens / Q000235:genetics*; D008969:Molecular Sequence Data; D012275:Oryza / Q000235:genetics / Q000378:metabolism*; D018515:Plant Leaves / Q000737:chemistry; D030821:Plants, Genetically Modified / Q000235:genetics / Q000378:metabolism*; D026723:Polyadenylation; D039981:Pyroglyphidae / Q000235:genetics*; D012333:RNA, Messenger / Q000378:metabolism; D012639:Seeds / Q000737:chemistry; D014158:Transcription, Genetic; D003313:Zea mays / Q000235:genetics*
D020413:3' Untranslated Regions; D000485:Allergens; C120054:GluB-1 protein, Oryza; D012333:RNA, Messenger; D005983:Glutens