Dengue virus antibodies in blood donors from an endemic area.
We evaluated the incidence of anti-Dengue virus (DENV) antibodies and dengue viremia in a region of Mexico with a high prevalence of dengue. DENV is the most important arthropod-borne virus in terms of human morbidity and mortality in America We tested 800 blood donors from a tertiary care teaching hospital that provides care in Northeast Mexico, to identify anti-DENV IgM and IgG antibodies by enzyme-linked immunosorbent assay (ELISA) and DENV genome by reverse transcription polymerase chain reaction (RT-PCR). In addition, routine tests for donors including Brucella, Hepatitis C virus (HCV), Venereal Disease Research Laboratory (VDRL), HIV-1 and HBsAg identification were performed. We found that 59% of donors were reactive for anti-DENV IgG and none of them had reported recent DENV infection; however, 16 (2%) were reactive for anti-DENV IgM antibodies. None of them were viremic at the time of donation. Routine tests showed that the prevalence of anti-Brucella was 0.71%, anti-HCV 0.71%, anti-HIV-1-2 0.14%, HBsAg 0.14% and VDRL test 0.57%. Although DENV transmission by blood transfusion had not been confirmed in Mexico, the finding of a high prevalence of anti-DENV IgM-positive donors with asymptomatic manifestations and the recent viremia reported in blood donors suggests that this route of transmission might be possible.
Rodríguez Rodríguez
D
D
Laboratory of Molecular Infectology, Department of Biochemistry and Molecular Medicine, School of Medicine and Hospital Universitario Dr. Jose E. Gonzalez, Universidad Autónoma de Nuevo León Monterrey, NL, Mexico.
Garza Rodríguez
M
M
Chavarria
A M
AM
Ramos-Jiménez
J
J
Rivera
M A
MA
Taméz
R C
RC
Farfan-Ale
J
J
Rivas-Estilla
A M
AM
D000293:Adolescent; D000328:Adult; D000368:Aged; D000907:Antibodies, Bacterial / Q000097:blood; D000914:Antibodies, Viral / Q000097:blood* / Q000276:immunology; D001782:Blood Donors; D002002:Brucella / Q000276:immunology; D003715:Dengue / Q000097:blood* / Q000453:epidemiology / Q000635:transmission; D003716:Dengue Virus / Q000276:immunology; D019353:Endemic Diseases; D005260:Female; D015497:HIV-1 / Q000276:immunology; D016174:Hepacivirus / Q000276:immunology; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood* / Q000276:immunology; D007075:Immunoglobulin M / Q000097:blood* / Q000276:immunology; D008297:Male; D008800:Mexico / Q000453:epidemiology; D008875:Middle Aged; D012749:Sexually Transmitted Diseases / Q000097:blood / Q000453:epidemiology / Q000276:immunology; D014766:Viremia / Q000097:blood / Q000453:epidemiology / Q000276:immunology
D000907:Antibodies, Bacterial; D000914:Antibodies, Viral; D007074:Immunoglobulin G; D007075:Immunoglobulin M