Vibrio breoganii sp. nov., a non-motile, alginolytic, marine bacterium within the Vibrio halioticoli clade.
Seven non-motile, facultatively anaerobic, alginolytic marine bacteria were isolated from the cultured clams Ruditapes philippinarum and Ruditapes decussatus. Phylogenetic analysis based on 16S rRNA gene sequences showed that these marine bacteria were closely related to the recently described species Vibrio comitans, Vibrio rarus and Vibrio inusitatus (>or=99.0 % sequence similarity). Phylogenetic analysis based on the housekeeping genes rpoA, recA and atpA grouped the isolates together and allocated them to the Vibrio halioticoli species group. Amplified fragment length polymorphism DNA fingerprinting also grouped them together and enabled them to be differentiated from recognized species of the V. halioticoli clade. DNA-DNA hybridizations showed that the isolates belonged to a novel species; phenotypic features such as the ability to grow at 4 degrees C and in the presence of 6 % NaCl also enabled them to be separated from other species. The DNA G+C content of RD 15.11(T) is 44.4 mol%. The genotypic and phenotypic data showed that the isolates represent a novel species in the V. halioticoli clade. The name Vibrio breoganii sp. nov. is proposed, with RD 15.11(T) (=CECT 7222(T) =LMG 23858(T)) as the type strain.
Beaz Hidalgo
Roxana
R
Departamento de Microbiología y Parasitología, Centro de Investigaciones Biológicas (CIBUS)-Facultad de Biología, Universidad de Santiago de Compostela, 15782, Santiago de Compostela, Spain.
Cleenwerck
Ilse
I
Balboa
Sabela
S
Prado
Susana
S
De Vos
Paul
P
Romalde
Jesús L
JL
D000464:Alginates / Q000378:metabolism*; D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics; D015373:Bacterial Typing Techniques; D001482:Base Composition; D049872:Bivalvia / Q000382:microbiology*; D016172:DNA Fingerprinting / Q000379:methods; D004269:DNA, Bacterial / Q000032:analysis; D020459:Genes, rRNA; D005838:Genotype; D020723:Glucuronic Acid / Q000378:metabolism; D006603:Hexuronic Acids / Q000378:metabolism; D008386:Marine Biology; D009693:Nucleic Acid Hybridization; D010641:Phenotype; D010802:Phylogeny; D012150:Polymorphism, Restriction Fragment Length; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013030:Spain; D013045:Species Specificity; D014733:Vibrio / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology
D000464:Alginates; D001426:Bacterial Proteins; D004269:DNA, Bacterial; D006603:Hexuronic Acids; D012336:RNA, Ribosomal, 16S; D020723:Glucuronic Acid