title: Polyphenol metabolites from colonic microbiota exert anti-inflammatory activity on different inflammation models.
abstract: The polyphenols in fruits and vegetables may be partly responsible for the health-promoting effects attributed to fruit and vegetable intake. Although their properties have been relatively well studied, the activity of their metabolites, produced after ingestion, has been poorly investigated. Thus, the aim of this work was to study the potential anti-inflammatory effect of 18 polyphenol metabolites, derived from colon microbiota. They were screened by measuring prostaglandin E(2) (PGE(2)) production by CCD-18 colon fibroblast cells stimulated with IL-1beta. Metabolites that inhibited more than 50% PGE(2) production were hydrocaffeic (HCAF), dihydroxyphenyl acetic (dOHPA), and hydroferulic acid (HFER), that subsequently were tested with the writhing and paw pressure test in rodents where all three compounds showed an anti-inflammatory effect. The effect of HCAF administered orally (50 mg/kg) was also tested in the dextran sodium sulfate (DSS)-induced colitis model. Weight loss and fecal water content were more pronounced in DSS rats than in DSS-HCAF treated rats. HCAF treatment diminished the expression of the cytokines IL-1beta, IL-8, and TNF-alpha, reduced malonyldialdehyde (MDA) levels and oxidative DNA damage (measured as 8-oxo-2'-deoxyguanosine levels) in distal colon mucosa. These results indicate that HCAF, dOHPA, and HFER have anti-inflammatory activity in vitro and in vivo.
authors: Department of Pharmacology, University of Florence, 50139 Florence, Italy. mar.larrosa@unifi.it
authors: Mar
authors: M
authors: Larrosa
authors: Cristina
authors: C
authors: Luceri
authors: Elisa
authors: E
authors: Vivoli
authors: Chiara
authors: C
authors: Pagliuca
authors: Maura
authors: M
authors: Lodovici
authors: Gloriano
authors: G
authors: Moneti
authors: Piero
authors: P
authors: Dolara
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D001419:Bacteria / Q000378:metabolism*; D003092:Colitis / Q000188:drug therapy*; D003106:Colon / Q000382:microbiology*; D015232:Dinoprostone / Q000096:biosynthesis; D005243:Feces / Q000737:chemistry; D005419:Flavonoids / Q000032:analysis / Q000494:pharmacology*; D008297:Male; D051379:Mice; D010636:Phenols / Q000032:analysis / Q000494:pharmacology*; D059808:Polyphenols; D051381:Rats; D011916:Rats, Inbred F344; D017208:Rats, Wistar; D017382:Reactive Oxygen Species
chemical_list: D000893:Anti-Inflammatory Agents; D005419:Flavonoids; D010636:Phenols; D059808:Polyphenols; D017382:Reactive Oxygen Species; D015232:Dinoprostone