Soluble Human Leukocyte Antigen class I antigen and interleukin-12 in hepatectomized patients.
BACKGROUND
Interleukin-12 (IL-12) has been shown to enhance the cytotoxic activity of NK cells and CTL. IL-12 also acts as a growth factor for activated NK, T and NKT cells. The soluble HLA class I (sHLA-I) has been reported to bind a killer-cell inhibitory receptor, which is expressed on the NK cell, and its signals inhibit NK cell-mediated cytotoxicity. Effects of fresh frozen plasma (FFP) on post-operative immune status have not yet been completely examined.


METHODS
Thirty consecutive patients taking a hepatectomy were enrolled. The levels of IL-12 and sHLA-I were examined by enzyme-linked immunosorbent assay.


RESULTS
The rate of complication after hepatectomy in the FFP-administered patients was higher than that in patients without FFP administration (P = 0.0358). Decreased IL-12 levels after surgery in patients without FFP administration recovered to the preoperative state earlier than those in patients with FFP administration (P < 0.05). The levels of sHLA-I in the FFP-administered patients were higher than those in the patients without FFP administration (P < 0.05).


CONCLUSIONS
Administration of FFP, which contains sHLA-I, affected the levels of sHLA-I after hepatectomy. Both high levels of sHLA-I and low levels of IL-12 could attenuate NK activities after hepatectomy, especially when FFP would be administered.
Shimura
Tatsuo
T
Department of Surgery, Fujioka General Hospital, Fujioka, Gunma, Japan. tatsuoshimura@gmail.com
Morinaga
Nobuhiro
N
Suzuki
Hideki
H
Araki
Kenichiro
K
Kobayashi
Tsutomu
T
Ishizaki
Masatoshi
M
Kuwano
Hiroyuki
H
D000276:Adjuvants, Immunologic; D000368:Aged; D016022:Case-Control Studies; D004797:Enzyme-Linked Immunosorbent Assay; D015788:HLA-A1 Antigen / Q000097:blood* / Q000502:physiology; D006498:Hepatectomy; D006801:Humans; D018664:Interleukin-12 / Q000276:immunology* / Q000494:pharmacology; D008103:Liver Cirrhosis / Q000276:immunology* / Q000601:surgery; D008875:Middle Aged; D010949:Plasma / Q000276:immunology*; D011184:Postoperative Period; D012995:Solubility; D065227:Transfusion Reaction
D000276:Adjuvants, Immunologic; D015788:HLA-A1 Antigen; D018664:Interleukin-12