Flow cytometric quantification of chlamydial infection in cell culture.
A flow cytometric method was developed, which allows fast and efficient analysis of cell cultures infected with chlamydiae. The proportion of positive cells increased with the infectious dose and correlated with chlamydia copy numbers calculated from real-time PCR. While retaining the advantages of single-cell analysis, flow cytometry allows handling of large sample numbers and counterstaining for additional marker proteins.
Grün
Michael
M
Institute of Nutrition, Friedrich-Schiller-University of Jena, Germany. michael.gruen@uni-jena.de
Liebisch
Marita
M
Sauerwein
Helga
H
Jahreis
Gerhard
G
Sachse
Konrad
K
D018929:Cell Culture Techniques; D002689:Chlamydia / Q000254:growth & development* / Q000302:isolation & purification*; D015169:Colony Count, Microbial / Q000379:methods*; D005434:Flow Cytometry / Q000379:methods*