Zwitterionic carboxybetaine polymer surfaces and their resistance to long-term biofilm formation.
In this work, we report a systematic study of zwitterionic poly(carboxybetaine methacrylate) (pCBMA) grafted from glass surfaces via atom transfer radical polymerization (ATRP) for their resistance to long-term bacterial biofilm formation. Results show that pCBMA-grafted surfaces are highly resistant to non-specific protein adsorption (fibrinogen and undiluted blood plasma) at 25, 30 and 37 degrees C. Long-term (over 24 h) colonization of two bacterial strains (Pseudomonas aeruginosa PAO1 and Pseudomonas putida strain 239) on pCBMA surface was studied using a parallel flow cell at 25, 30 and 37 degrees C. Uncoated glass cover slips were chosen as the positive reference. Results show that pCBMA coatings reduced long-term biofilm formation of P. aeruginosa up to 240 h by 95% at 25 degrees C and for 64 h by 93% at 37 degrees C, and suppressed P. putida biofilm accumulation up to 192 h by 95% at 30 degrees C, with respect to the glass reference. The ability of pCBMA coatings to resist non-specific protein adsorption and significantly retard bacterial biofilm formation makes it a very promising material for biomedical and industrial applications.
Cheng
Gang
G
Department of Chemical Engineering, University of Washington, Seattle, WA 98195, USA.
Li
Guozhu
G
Xue
Hong
H
Chen
Shengfu
S
Bryers
James D
JD
Jiang
Shaoyi
S
D000327:Adsorption; D001622:Betaine / Q000737:chemistry* / Q000494:pharmacology*; D018441:Biofilms / Q000187:drug effects* / Q000254:growth & development; D020099:Coated Materials, Biocompatible / Q000737:chemistry* / Q000494:pharmacology*; D005340:Fibrinogen / Q000737:chemistry; D005898:Glass / Q000737:chemistry; D006801:Humans; D010949:Plasma / Q000737:chemistry; D011109:Polymethacrylic Acids / Q000737:chemistry* / Q000494:pharmacology*; D011550:Pseudomonas aeruginosa / Q000187:drug effects / Q000254:growth & development; D016958:Pseudomonas putida / Q000187:drug effects / Q000254:growth & development; D013499:Surface Properties
D020099:Coated Materials, Biocompatible; D011109:Polymethacrylic Acids; C517178:polycarboxybetaine methacrylate; D001622:Betaine; D005340:Fibrinogen