Bile acid derivatives from a sponge-associated bacterium Psychrobacter sp.
In our search for bioactive metabolites from a marine sponge-associated bacterium Psychrobacter sp., a new bile acid derivative (1), which was assumed to be an artifact, were isolated along with six known (2-7) compounds by bioactivity-guided fractionation. Elucidation of the structure of the new compound was done using a combination of NMR ((1)H, (13)C, HMBC, HSQC, and COSY) and MS spectroscopy. Compound 1 exhibited moderate suppressive effects on both NO and IL-6 production at a concentration of 200 microM (87.3 microg/mL) without significant cytotoxicity against cells. Compounds 2-5 and 7 showed selective inhibitory activity against several human pathogenic bacterial strains at the low concentration of 30 microg/well. In a cytotoxicity evaluation, only compound 7 showed mild cytotoxicity against five human solid tumor cell lines (A-549, SK-OV-3, SK-MEL-2, XF-498, and HCT-15) with ED(50) values in the range of 11-14 microg/mL.
Li
Huayue
H
College of Pharmacy, Pusan National University, Busan 609-735, Korea.
Shinde
Pramod B
PB
Lee
Hye Ja
HJ
Yoo
Eun Sook
ES
Lee
Chong-O
CO
Hong
Jongki
J
Choi
Sang Ho
SH
Jung
Jee H
JH
D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D000893:Anti-Inflammatory Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D000970:Antineoplastic Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D001647:Bile Acids and Salts / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D002460:Cell Line; D045744:Cell Line, Tumor; D002793:Cholic Acids / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D004354:Drug Screening Assays, Antitumor; D006801:Humans; D015850:Interleukin-6 / Q000378:metabolism; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism; D051379:Mice; D008956:Models, Chemical; D015394:Molecular Structure; D009569:Nitric Oxide / Q000378:metabolism; D011161:Porifera / Q000382:microbiology*; D044096:Psychrobacter / Q000737:chemistry*
C542605:3-dimethoxy-12alpha-hydroxycholanic acid; D000900:Anti-Bacterial Agents; D000893:Anti-Inflammatory Agents; D000970:Antineoplastic Agents; D001647:Bile Acids and Salts; D002793:Cholic Acids; D015850:Interleukin-6; D009569:Nitric Oxide