title: AtCYP20-2 is an auxiliary protein of the chloroplast NAD(P)H dehydrogenase complex.
abstract: AtCYP20-2 is one of 16 immunophilins in thylakoid lumen. The presence of the isomerase domain in AtCYP20-2, an enrichment of AtCYP20-2 in the stroma membranes and it's co-migration with NAD(P)H dehydrogenase (NDH) in native gels provide evidence that AtCYP20-2 is an auxiliary protein of NDH. When different NDH mutants were studied, AtCYP20-2 was found to be strongly reduced especially in mutants deficient in the membrane domain of NDH, thus suggesting a role in the assembly of NDH hydrophobic domain. Lack of AtCYP20-2, however, did not lead to severe malfunction of NDH, indicating redundancy in the function of lumenal immunophilins.
authors: Department of Biology, Plant Physiology and Molecular Biology, University of Turku, FI-20014 Turku, Finland.
authors: Sari
authors: S
authors: Sirpiö
authors: Maija
authors: M
authors: Holmström
authors: Natalia
authors: N
authors: Battchikova
authors: Eva-Mari
authors: EM
authors: Aro
mesh_terms: D017360:Arabidopsis / Q000166:cytology / Q000201:enzymology*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D002736:Chloroplasts / Q000201:enzymology*; D021983:Cyclophilins / Q000235:genetics / Q000378:metabolism*; D009252:NADPH Dehydrogenase / Q000235:genetics / Q000378:metabolism*; D010788:Photosynthesis / Q000502:physiology; D020524:Thylakoids / Q000201:enzymology / Q000648:ultrastructure
chemical_list: D029681:Arabidopsis Proteins; D009252:NADPH Dehydrogenase; C523353:CYP20-2 protein, Arabidopsis; D021983:Cyclophilins