Optically sectioned fluorescence endomicroscopy with hybrid-illumination imaging through a flexible fiber bundle.
We present an endomicroscope apparatus that exhibits out-of-focus background rejection based on wide-field illumination through a flexible imaging fiber bundle. Our technique, called HiLo microscopy, involves acquiring two images, one with grid-pattern illumination and another with standard uniform illumination. An evaluation of the image contrast with grid-pattern illumination provides an optically sectioned image with low resolution. This is complemented with high-resolution information from the uniform illumination image, leading to a full-resolution image that is optically sectioned. HiLo endomicroscope movies are presented of fluorescently labeled rat colonic mucosa.
Santos
Silvia
S
Chu
Kengyeh K
KK
Lim
Daryl
D
Bozinovic
Nenad
N
Ford
Tim N
TN
Hourtoule
Claire
C
Bartoo
Aaron C
AC
Singh
Satish K
SK
Mertz
Jerome
J
D000165:Acridine Orange; D000818:Animals; D003106:Colon / Q000033:anatomy & histology; D004724:Endoscopy / Q000379:methods*; D005336:Fiber Optic Technology / Q000295:instrumentation / Q000379:methods*; D007089:Image Enhancement / Q000379:methods; D007413:Intestinal Mucosa / Q000033:anatomy & histology; D008856:Microscopy, Fluorescence / Q000295:instrumentation / Q000379:methods*; D051381:Rats
D000165:Acridine Orange