Gene expression analysis of Paracoccidioides brasiliensis transition from conidium to yeast cell.
Paracoccidioides brasiliensis infectious process relies on the initial expression of virulence factors that are assumed to be controlled by molecular mechanisms through which the conidia and/or mycelial fragments convert to yeast cells. In order to analyze the profile of the thermally-induced dimorphic gene expression, 48 h C-L transition cultures which had been incubated at 36 degrees C were studied. By this time approximately 50% of the conidial population had already reverted to yeast form cells. At this transition time, an EST-Orestes library was constructed and characterized. As a result, 79 sequences were obtained, of which 39 (49.4%) had not been described previously in other libraries of this fungus and which could represent novel exclusive C-Y transition genes. Two of these sequences are, among others, cholestanol delta-isomerase, and electron transfer flavoprotein-ubiquinoneoxidoreductase (ETF-QO). The other 40/79 (50.6%) sequences were shared with Mycelia (M), Yeast (Y) or Mycelia to yest transition (M-Y) libraries. An important component of this group of sequences is a putative response regulator receiver SKN7, a protein of high importance in stress adaptation and a regulator of virulence in some bacteria and fungi. This is the first report identifying genes expressed during the C-Y transition process, the initial step required to understand the natural history of P. brasiliensis conidia induced infection.
García
Ana M
AM
Unidad de Biología Celular y Molecular Corporación para Investigaciones Biológicas, Medellín, Colombia. agarcia@cib.org.co
Hernández
Orville
O
Aristizabal
Beatriz H
BH
De Souza Bernardes
Luciano A
LA
Puccia
Rosana
R
Naranjo
Tony W
TW
Goldman
Gustavo H
GH
Goldman
Maria H
MH
Cano
Luz E
LE
Restrepo
Angela
A
McEwen
Juan G
JG
D005656:Fungal Proteins / Q000096:biosynthesis / Q000235:genetics; D020869:Gene Expression Profiling; D015966:Gene Expression Regulation, Fungal; D025282:Mycelium / Q000235:genetics / Q000254:growth & development / Q000502:physiology; D010228:Paracoccidioides / Q000235:genetics / Q000254:growth & development / Q000502:physiology*; D013172:Spores, Fungal / Q000235:genetics / Q000254:growth & development / Q000502:physiology; D015003:Yeasts / Q000235:genetics / Q000254:growth & development / Q000502:physiology
D005656:Fungal Proteins